CHOROIDAL THICKNESS IN DIABETIC RETINOPATHY ASSESSED WITH SWEPT-SOURCE OPTICAL COHERENCE TOMOGRAPHY.

PubMed

Laíns, Inês; Talcott, Katherine E; Santos, Ana R; Marques, João H; Gil, Pedro; Gil, João; Figueira, João; Husain, Deeba; Kim, Ivana K; Miller, Joan W; Silva, Rufino; Miller, John B

2018-01-01

To compare the choroidal thickness (CT) of diabetic eyes (different stages of disease) with controls, using swept-source optical coherence tomography. A multicenter, prospective, cross-sectional study of diabetic and nondiabetic subjects using swept-source optical coherence tomography imaging. Choroidal thickness maps, according to the nine Early Treatment Diabetic Retinopathy Study (ETDRS) subfields, were obtained using automated software. Mean CT was calculated as the mean value within the ETDRS grid, and central CT as the mean in the central 1 mm. Diabetic eyes were divided into four groups: no diabetic retinopathy (No DR), nonproliferative DR (NPDR), NPDR with diabetic macular edema (NPDR + DME), and proliferative DR (PDR). Multilevel mixed linear models were performed for analyses. The authors included 50 control and 160 diabetic eyes (n = 27 No DR, n = 51 NPDR, n = 61 NPDR + DME, and n = 21 PDR). Mean CT (ß = -42.9, P = 0.022) and central CT (ß = -50.2, P = 0.013) were statistically significantly thinner in PDR eyes compared with controls, even after adjusting for confounding factors. Controlling for age, DR eyes presented a significantly decreased central CT than diabetic eyes without retinopathy (β = -36.2, P = 0.009). Swept-source optical coherence tomography demonstrates a significant reduction of CT in PDR compared with controls. In the foveal region, the choroid appears to be thinner in DR eyes than in diabetic eyes without retinopathy.

Noninvasive Visualization and Analysis of the Human Parafoveal Capillary Network Using Swept Source OCT Optical Microangiography.

PubMed

Kuehlewein, Laura; Tepelus, Tudor C; An, Lin; Durbin, Mary K; Srinivas, Sowmya; Sadda, Srinivas R

2015-06-01

We characterized the foveal avascular zone (FAZ) and the parafoveal capillary network in healthy subjects using swept source OCT optical microangiography (OMAG). We acquired OMAG images of the macula of 19 eyes (13 healthy individuals) using a prototype swept source laser OCT. En face images of the retinal vasculature were generated for superficial and deep inner retinal layers (SRL/DRL) in regions of interest 250 (ROI-250) and 500 (ROI-500) μm from the FAZ border. The mean area (mm2) of the FAZ was 0.304 ± 0.132 for the SRL and 0.486 ± 0.162 for the DRL (P < 0.001). Mean vessel density (%) was 67.3 ± 6.4 for the SRL and 34.5 ± 8.6 for the DRL in the ROI-250 (P < 0.001), and 74.2 ± 3.9 for the SRL and 72.3 ± 4.9 for the DRL in the ROI-500 (P = 0.160). Swept source OMAG images of healthy subjects allowed analysis of the FAZ and the density of the parafoveal capillary network at different retinal layers.

Subgingival calculus imaging based on swept-source optical coherence tomography

NASA Astrophysics Data System (ADS)

Hsieh, Yao-Sheng; Ho, Yi-Ching; Lee, Shyh-Yuan; Lu, Chih-Wei; Jiang, Cho-Pei; Chuang, Ching-Cheng; Wang, Chun-Yang; Sun, Chia-Wei

2011-07-01

We characterized and imaged dental calculus using swept-source optical coherence tomography (SS-OCT). The refractive indices of enamel, dentin, cementum, and calculus were measured as 1.625 +/- 0.024, 1.534 +/- 0.029, 1.570 +/- 0.021, and 2.097 +/- 0.094, respectively. Dental calculus leads strong scattering properties, and thus, the region can be identified from enamel with SS-OCT imaging. An extracted human tooth with calculus is covered with gingiva tissue as an in vitro sample for tomographic imaging.

Long-range and wide field of view optical coherence tomography for in vivo 3D imaging of large volume object based on akinetic programmable swept source.

PubMed

Song, Shaozhen; Xu, Jingjiang; Wang, Ruikang K

2016-11-01

Current optical coherence tomography (OCT) imaging suffers from short ranging distance and narrow imaging field of view (FOV). There is growing interest in searching for solutions to these limitations in order to expand further in vivo OCT applications. This paper describes a solution where we utilize an akinetic swept source for OCT implementation to enable ~10 cm ranging distance, associated with the use of a wide-angle camera lens in the sample arm to provide a FOV of ~20 x 20 cm 2 . The akinetic swept source operates at 1300 nm central wavelength with a bandwidth of 100 nm. We propose an adaptive calibration procedure to the programmable akinetic light source so that the sensitivity of the OCT system over ~10 cm ranging distance is substantially improved for imaging of large volume samples. We demonstrate the proposed swept source OCT system for in vivo imaging of entire human hands and faces with an unprecedented FOV (up to 400 cm 2 ). The capability of large-volume OCT imaging with ultra-long ranging and ultra-wide FOV is expected to bring new opportunities for in vivo biomedical applications.

Long-range and wide field of view optical coherence tomography for in vivo 3D imaging of large volume object based on akinetic programmable swept source

PubMed Central

Song, Shaozhen; Xu, Jingjiang; Wang, Ruikang K.

2016-01-01

Current optical coherence tomography (OCT) imaging suffers from short ranging distance and narrow imaging field of view (FOV). There is growing interest in searching for solutions to these limitations in order to expand further in vivo OCT applications. This paper describes a solution where we utilize an akinetic swept source for OCT implementation to enable ~10 cm ranging distance, associated with the use of a wide-angle camera lens in the sample arm to provide a FOV of ~20 x 20 cm2. The akinetic swept source operates at 1300 nm central wavelength with a bandwidth of 100 nm. We propose an adaptive calibration procedure to the programmable akinetic light source so that the sensitivity of the OCT system over ~10 cm ranging distance is substantially improved for imaging of large volume samples. We demonstrate the proposed swept source OCT system for in vivo imaging of entire human hands and faces with an unprecedented FOV (up to 400 cm2). The capability of large-volume OCT imaging with ultra-long ranging and ultra-wide FOV is expected to bring new opportunities for in vivo biomedical applications. PMID:27896012

Longitudinal Evaluation of Cornea With Swept-Source Optical Coherence Tomography and Scheimpflug Imaging Before and After Lasik

PubMed Central

Chan, Tommy C.Y.; Biswas, Sayantan; Yu, Marco; Jhanji, Vishal

2015-01-01

Abstract Swept-source optical coherence tomography (OCT) is the latest advancement in anterior segment imaging. There are limited data regarding its performance after laser in situ keratomileusis (LASIK). We compared the reliability of swept-source OCT and Scheimpflug imaging for evaluation of corneal parameters in refractive surgery candidates with myopia or myopic astigmatism. Three consecutive measurements were obtained preoperatively and 1 year postoperatively using swept-source OCT and Scheimpflug imaging. The study parameters included central corneal thickness (CCT), thinnest corneal thickness (TCT), keratometry at steep (Ks) and flat (Kf) axes, mean keratometry (Km), and, anterior and posterior best fit spheres (Ant and Post BFS). The main outcome measures included reliability of measurements before and after LASIK was evaluated using intraclass correlation coefficient (ICC) and reproducibility coefficients (RC). Association between the mean value of corneal parameters with age, spherical equivalent (SEQ), and residual bed thickness (RBT) and association of variance heterogeneity of corneal parameters and these covariates were analyzed. Twenty-six right eyes of 26 participants (mean age, 32.7 ± 6.9 yrs; mean SEQ, −6.27 ± 1.67 D) were included. Preoperatively, swept-source OCT demonstrated significantly higher ICC for Ks, CCT, TCT, and Post BFS (P ≤ 0.016), compared with Scheimpflug imaging. Swept-source OCT demonstrated significantly smaller RC values for CCT, TCT, and Post BFS (P ≤ 0.001). After LASIK, both devices had significant differences in measurements for all corneal parameters (P ≤ 0.015). Swept-source OCT demonstrated a significantly higher ICC and smaller RC for all measurements, compared with Scheimpflug imaging (P ≤ 0.001). Association of variance heterogeneity was only found in pre-LASIK Ant BFS and post-LASIK Post BFS for swept-source OCT, whereas significant association of variance heterogeneity was noted for all measurements except Ks and Km for Scheimpflug imaging. This study reported higher reliability of swept-source OCT for post-LASIK corneal measurements, as compared with Scheimpflug imaging. The reliability of corneal parameters measured with Scheimpflug imaging after LASIK was not consistent across different age, SEQ, and RBT measurements. These factors need to be considered during follow-up and evaluation of post-LASIK patients for further surgical procedures. PMID:26222852

Subgingival calculus imaging based on swept-source optical coherence tomography.

PubMed

Hsieh, Yao-Sheng; Ho, Yi-Ching; Lee, Shyh-Yuan; Lu, Chih-Wei; Jiang, Cho-Pei; Chuang, Ching-Cheng; Wang, Chun-Yang; Sun, Chia-Wei

2011-07-01

We characterized and imaged dental calculus using swept-source optical coherence tomography (SS-OCT). The refractive indices of enamel, dentin, cementum, and calculus were measured as 1.625 ± 0.024, 1.534 ± 0.029, 1.570 ± 0.021, and 2.097 ± 0.094, respectively. Dental calculus leads strong scattering properties, and thus, the region can be identified from enamel with SS-OCT imaging. An extracted human tooth with calculus is covered with gingiva tissue as an in vitro sample for tomographic imaging.

Quantitative phase imaging of living cells with a swept laser source

NASA Astrophysics Data System (ADS)

Chen, Shichao; Zhu, Yizheng

2016-03-01

Digital holographic phase microscopy is a well-established quantitative phase imaging technique. However, interference artifacts from inside the system, typically induced by elements whose optical thickness are within the source coherence length, limit the imaging quality as well as sensitivity. In this paper, a swept laser source based technique is presented. Spectra acquired at a number of wavelengths, after Fourier Transform, can be used to identify the sources of the interference artifacts. With proper tuning of the optical pathlength difference between sample and reference arms, it is possible to avoid these artifacts and achieve sensitivity below 0.3nm. Performance of the proposed technique is examined in live cell imaging.

VASCULAR ABNORMALITIES IN DIABETIC RETINOPATHY ASSESSED WITH SWEPT-SOURCE OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY WIDEFIELD IMAGING.

PubMed

Schaal, Karen B; Munk, Marion R; Wyssmueller, Iris; Berger, Lieselotte E; Zinkernagel, Martin S; Wolf, Sebastian

2017-11-10

To detect vascular abnormalities in diabetic retinopathy using swept-source optical coherence tomography angiography (SS-OCTA) widefield images, and to compare the findings with color fundus photographs (CFPs) using Early Treatment Diabetic Retinopathy Study severity grading. 3 mm × 3 mm and 12 mm × 12 mm scans were acquired to cover 70° to 80° of the posterior pole using a 100-kHz SS-OCTA instrument. Two masked graders assessed the presence of vascular abnormalities on SS-OCTA and the Early Treatment Diabetic Retinopathy Study level on CFP. The grading results were then compared. A total of 120 diabetic eyes (60 patients) were imaged with the SS-OCTA instrument. Cohort 1 (91 eyes; SS-OCTA grading only) showed microaneurysms in 91% (n = 83), intraretinal microvascular abnormalities in 79% (n = 72), and neovascularization in 21% (n = 19) of cases. Cohort 2 (52 eyes; CFP grading compared with SS-OCTA) showed microaneurysms on CFP in 90% (n = 47) and on SS-OCTA in 96% (n = 50) of cases. Agreement in intraretinal microvascular abnormality detection was fair (k = 0.2). Swept-source optical coherence tomography angiography detected 50% of intraretinal microvascular abnormality cases (n = 26), which were missed on CFP. Agreement in detecting neovascularization was moderate (k = 0.5). Agreement in detection of diabetic retinopathy features on CFP and SS-OCTA varies depending on the vascular changes examined. Swept-source optical coherence tomography angiography shows a higher detection rate of intraretinal microvascular abnormalities (P = 0.039), compared with Early Treatment Diabetic Retinopathy Study grading.

Compact akinetic swept source optical coherence tomography angiography at 1060 nm supporting a wide field of view and adaptive optics imaging modes of the posterior eye.

PubMed

Salas, Matthias; Augustin, Marco; Felberer, Franz; Wartak, Andreas; Laslandes, Marie; Ginner, Laurin; Niederleithner, Michael; Ensher, Jason; Minneman, Michael P; Leitgeb, Rainer A; Drexler, Wolfgang; Levecq, Xavier; Schmidt-Erfurth, Ursula; Pircher, Michael

2018-04-01

Imaging of the human retina with high resolution is an essential step towards improved diagnosis and treatment control. In this paper, we introduce a compact, clinically user-friendly instrument based on swept source optical coherence tomography (SS-OCT). A key feature of the system is the realization of two different operation modes. The first operation mode is similar to conventional OCT imaging and provides large field of view (FoV) images (up to 45° × 30°) of the human retina and choroid with standard resolution. The second operation mode enables it to optically zoom into regions of interest with high transverse resolution using adaptive optics (AO). The FoV of this second operation mode (AO-OCT mode) is 3.0° × 2.8° and enables the visualization of individual retinal cells such as cone photoreceptors or choriocapillaris. The OCT engine is based on an akinetic swept source at 1060 nm and provides an A-scan rate of 200 kHz. Structural as well as angiographic information can be retrieved from the retina and choroid in both operational modes. The capabilities of the prototype are demonstrated in healthy and diseased eyes.

Swept-source optical coherence tomography of lower limb wound healing with histopathological correlation

NASA Astrophysics Data System (ADS)

Barui, Ananya; Banerjee, Provas; Patra, Rusha; Das, Raunak Kumar; Dhara, Santanu; Dutta, Pranab K.; Chatterjee, Jyotirmoy

2011-02-01

Direct noninvasive visualization of wound bed with depth information is important to understand the tissue repair. We correlate skin swept-source-optical coherence tomography (OCT) with histopathological and immunohistochemical evaluation on traumatic lower limb wounds under honey dressing to compare and assess the tissue repair features acquired noninvasively and invasively. Analysis of optical biopsy identifies an uppermost brighter band for stratum corneum with region specific thickness (p < 0.0001) and gray-level intensity (p < 0.0001) variation. Below the stratum corneum, variation in optical intensities is remarkable in different regions of the wound bed. Correlation between OCT and microscopic observations are explored especially in respect to progressive growth and maturation of the epithelial and subepithelial components. Characteristic transition of uniform hypolucid band in OCT image for depigmented zone to wavy highly lucid band in the pigmented zone could be directly correlated with the microscopic findings. The transformation of prematured epithelium of depigmented area, with low expression of E-cadherin, to matured epithelium with higher E-cadherin expression in pigmented zone, implicated plausible change in their optical properties as depicted in OCT. This correlated evaluation of multimodal images demonstrates applicability of swept-source-OCT in wound research and importance of integrated approach in validation of new technology.

Compact akinetic swept source optical coherence tomography angiography at 1060 nm supporting a wide field of view and adaptive optics imaging modes of the posterior eye

PubMed Central

Salas, Matthias; Augustin, Marco; Felberer, Franz; Wartak, Andreas; Laslandes, Marie; Ginner, Laurin; Niederleithner, Michael; Ensher, Jason; Minneman, Michael P.; Leitgeb, Rainer A.; Drexler, Wolfgang; Levecq, Xavier; Schmidt-Erfurth, Ursula; Pircher, Michael

2018-01-01

Imaging of the human retina with high resolution is an essential step towards improved diagnosis and treatment control. In this paper, we introduce a compact, clinically user-friendly instrument based on swept source optical coherence tomography (SS-OCT). A key feature of the system is the realization of two different operation modes. The first operation mode is similar to conventional OCT imaging and provides large field of view (FoV) images (up to 45° × 30°) of the human retina and choroid with standard resolution. The second operation mode enables it to optically zoom into regions of interest with high transverse resolution using adaptive optics (AO). The FoV of this second operation mode (AO-OCT mode) is 3.0° × 2.8° and enables the visualization of individual retinal cells such as cone photoreceptors or choriocapillaris. The OCT engine is based on an akinetic swept source at 1060 nm and provides an A-scan rate of 200 kHz. Structural as well as angiographic information can be retrieved from the retina and choroid in both operational modes. The capabilities of the prototype are demonstrated in healthy and diseased eyes. PMID:29675326

Method to calibrate phase fluctuation in polarization-sensitive swept-source optical coherence tomography

NASA Astrophysics Data System (ADS)

Lu, Zenghai; Kasaragod, Deepa K.; Matcher, Stephen J.

2011-07-01

We present a phase fluctuation calibration method for polarization-sensitive swept-source optical coherence tomography (PS-SS-OCT) using continuous polarization modulation. The method uses a low-voltage broadband polarization modulator driven by a synchronized sinusoidal burst waveform rather than an asynchronous waveform, together with the removal of the global phases of the measured Jones matrices by the use of matrix normalization. This makes it possible to average the measured Jones matrices to remove the artifact due to the speckle noise of the signal in the sample without introducing auxiliary optical components into the sample arm. This method was validated on measurements of an equine tendon sample by the PS-SS-OCT system.

Polarization sensitive corneal and anterior segment swept-source optical coherence tomography

NASA Astrophysics Data System (ADS)

Lim, Yiheng; Yamanari, Masahiro; Yasuno, Yoshiaki

2010-02-01

We develop a compact polarization sensitive corneal and anterior segment swept-source optical coherence tomography (PS-CAS- OCT) for evaluating the usefulness of PS-OCT, and enabling large scale studies in the tissue properties of normal and diseased eyes using the benefits of the PS-OCT, which provides better tissue discrimination compared to the conventional OCT by visualizing the fibrous tissues in the anterior eye segment. Our polarization-sensitive interferometer is size reduced into a 19 inch box for the portability and the probe is integrated into a position adjustable scanning head for the usability of our system.

External amplification of OCT swept-sources for challenging applications: from 10 mW to more than 120 mW

NASA Astrophysics Data System (ADS)

Rivard, Maxime; Villeneuve, Alain; Lamouche, Guy

2017-02-01

For bioimaging applications, commercial swept-sources currently provide enough power (tens of milliwatts) insuring good imaging condition without damaging the tissues. For industrial applications, more power is needed since the amount of light collected can be very low due to challenging measurement conditions or due to poor sample reflectivity. To address this challenge, we explore three different setups to externally amplify the output of a commercial swept-source: a booster semiconductor optical amplifier (BOA), an erbium-doped fiber amplifier (EDFA) and a combination of both. These external amplification setups allow the exploration of emerging OCT applications without the need to develop new hardware.

Acousto-Optic–Based Wavelength-Comb-Swept Laser for Extended Displacement Measurements

PubMed Central

Park, Nam Su; Chun, Soo Kyung; Han, Ga-Hee; Kim, Chang-Seok

2017-01-01

We demonstrate a novel wavelength-comb-swept laser based on two intra-cavity filters: an acousto-optic tunable filter (AOTF) and a Fabry-Pérot etalon filter. The AOTF is used for the tunable selection of the output wavelength with time and the etalon filter for the narrowing of the spectral linewidth to extend the coherence length. Compared to the conventional wavelength-swept laser, the acousto-optic–based wavelength-comb-swept laser (WCSL) can extend the measureable range of displacement measurements by decreasing the sensitivity roll-off of the point spread function. Because the AOTF contains no mechanical moving parts to select the output wavelength acousto-optically, the WCSL source has a high wavenumber (k) linearity of R2 = 0.9999 to ensure equally spaced wavelength combs in the wavenumber domain. PMID:28362318

The advantages of a swept source optical coherence tomography system in the evaluation of occlusal disorders

NASA Astrophysics Data System (ADS)

Marcauteanu, Corina; Bradu, Adrian; Sinescu, Cosmin; Topala, Florin Ionel; Negrutiu, Meda Lavinia; Duma, Virgil Florin; Podoleanu, Adrian Gh.

2014-01-01

Occlusal disorders are characterized by multiple dental and periodontal signs. Some of these are reversible (such as excessive tooth mobility, fremitus, tooth pain, migration of teeth in the absence of periodontitis), some are not (pathological occlusal/incisal wear, abfractions, enamel cracks, tooth fractures, gingival recessions). In this paper we prove the advantages of a fast swept source OCT system in the diagnosis of pathological incisal wear, a key sign of the occlusal disorders. On 15 extracted frontal teeth four levels of pathological incisal wear facets were artificially created. After every level of induced defect, OCT scanning was performed. B scans were acquired and 3D reconstructions were generated. A swept source OCT instrument is used in this study. The swept source is has a central wavelength of 1050 nm and a sweeping rate of 100 kHz. A depth resolution determined by the swept source of 12 μm in air was experimentally measured. The pathological incisal wear is qualitatively observed on the B-scans as 2D images and 3D reconstructions (volumes). For quantitative evaluations of volumes, we used the Image J software. Our swept source OCT system has several advantages, including the ability to measure (in air) a minimal volume of 2352 μm3 and to collect high resolution volumetric images in 2.5 s. By calculating the areas of the amount of lost tissue corresponding to each difference of B-scans, the final volumes of incisal wear were obtained. This swept source OCT method is very useful for the dynamic evaluation of pathological incisal wear.

Quantitative evaluation of dental abfraction and attrition using a swept-source optical coherence tomography system.

PubMed

Marcauteanu, Corina; Bradu, Adrian; Sinescu, Cosmin; Topala, Florin Ionel; Negrutiu, Meda Lavinia; Podoleanu, Adrian Gh

2014-02-01

A fast swept-source optical coherence tomography (SS-OCT) system is employed to acquire volumes of dental tissue, in order to monitor the temporal evolution of dental wear. An imaging method is developed to evaluate the volume of tissue lost in ex vivo artificially induced abfractions and attritions. The minimal volume (measured in air) that our system could measure is 2352 μm3. A volume of 25,000 A-scans is collected in 2.5 s. All these recommend the SS-OCT method as a valuable tool for dynamic evaluation of the abfraction and attrition with remarkable potential for clinical use.

Off-axis full-field swept-source optical coherence tomography using holographic refocusing

NASA Astrophysics Data System (ADS)

Hillmann, Dierck; Franke, Gesa; Hinkel, Laura; Bonin, Tim; Koch, Peter; Hüttmann, Gereon

2013-03-01

We demonstrate a full-field swept-source OCT using an off-axis geometry of the reference illumination. By using holographic refocusing techniques, a uniform lateral resolution is achieved over the measurement depth of approximately 80 Rayleigh lengths. Compared to a standard on-axis setup, artifacts and autocorrelation signals are suppressed and the measurement depth is doubled by resolving the complex conjugate ambiguity. Holographic refocusing was done efficiently by Fourier-domain resampling as demonstrated before in inverse scattering and holoscopy. It allowed to reconstruct a complete volume with about 10μm resolution over the complete measurement depth of more than 10mm. Off-axis full-field swept-source OCT enables high measurement depths, spanning many Rayleigh lengths with reduced artifacts.

Increasing signal-to-noise ratio of swept-source optical coherence tomography by oversampling in k-space

NASA Astrophysics Data System (ADS)

Nagib, Karim; Mezgebo, Biniyam; Thakur, Rahul; Fernando, Namal; Kordi, Behzad; Sherif, Sherif

2018-03-01

Optical coherence tomography systems suffer from noise that could reduce ability to interpret reconstructed images correctly. We describe a method to increase the signal-to-noise ratio of swept-source optical coherence tomography (SSOCT) using oversampling in k-space. Due to this oversampling, information redundancy would be introduced in the measured interferogram that could be used to reduce white noise in the reconstructed A-scan. We applied our novel scaled nonuniform discrete Fourier transform to oversampled SS-OCT interferograms to reconstruct images of a salamander egg. The peak-signal-to-noise (PSNR) between the reconstructed images using interferograms sampled at 250MS/s andz50MS/s demonstrate that this oversampling increased the signal-to-noise ratio by 25.22 dB.

A method to calibrate phase fluctuation in polarization-sensitive swept-source optical coherence tomography

NASA Astrophysics Data System (ADS)

Lu, Zenghai; Kasaragod, Deepa K.; Matcher, Stephen J.

2011-06-01

A phase fluctuation calibration method is presented for polarization-sensitive swept-source optical coherence tomography (PS-SS-OCT) using continuous polarization modulation. The method consists of the generation of a continuous triggered tone-burst waveform rather than an asynchronous waveform by use of a function generator and the removal of the global phases of the measured Jones matrices by use of matrix normalization. This could remove the use of auxiliary optical components for the phase fluctuation compensation in the system, which reduces the system complexity. Phase fluctuation calibration is necessary to obtain the reference Jones matrix by averaging the measured Jones matrices at sample surfaces. Measurements on an equine tendon sample were made by the PS-SS-OCT system to validate the proposed method.

Multimodal ophthalmic imaging using swept source spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography

NASA Astrophysics Data System (ADS)

Malone, Joseph D.; El-Haddad, Mohamed T.; Tye, Logan A.; Majeau, Lucas; Godbout, Nicolas; Rollins, Andrew M.; Boudoux, Caroline; Tao, Yuankai K.

2016-03-01

Scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT) benefit clinical diagnostic imaging in ophthalmology by enabling in vivo noninvasive en face and volumetric visualization of retinal structures, respectively. Spectrally encoding methods enable confocal imaging through fiber optics and reduces system complexity. Previous applications in ophthalmic imaging include spectrally encoded confocal scanning laser ophthalmoscopy (SECSLO) and a combined SECSLO-OCT system for image guidance, tracking, and registration. However, spectrally encoded imaging suffers from speckle noise because each spectrally encoded channel is effectively monochromatic. Here, we demonstrate in vivo human retinal imaging using a swept source spectrally encoded scanning laser ophthalmoscope and OCT (SSSESLO- OCT) at 1060 nm. SS-SESLO-OCT uses a shared 100 kHz Axsun swept source, shared scanner and imaging optics, and are detected simultaneously on a shared, dual channel high-speed digitizer. SESLO illumination and detection was performed using the single mode core and multimode inner cladding of a double clad fiber coupler, respectively, to preserve lateral resolution while improving collection efficiency and reducing speckle contrast at the expense of confocality. Concurrent en face SESLO and cross-sectional OCT images were acquired with 1376 x 500 pixels at 200 frames-per-second. Our system design is compact and uses a shared light source, imaging optics, and digitizer, which reduces overall system complexity and ensures inherent co-registration between SESLO and OCT FOVs. En face SESLO images acquired concurrent with OCT cross-sections enables lateral motion tracking and three-dimensional volume registration with broad applications in multivolume OCT averaging, image mosaicking, and intraoperative instrument tracking.

Electro-optic KTN Devices

NASA Astrophysics Data System (ADS)

Yagi, Shogo; Fujiura, Kazuo

We have grown KTN crystals with optical quality, and developed high-speed beam deflectors and variable focal length lenses based on KTN's large electro-optic effect.　Furthermore, by using the KTN beam deflectors, we have developed a swept light source for OCT operable at 200 kHz.

Imaging of noncarious cervical lesions by means of a fast swept source optical coherence tomography system

NASA Astrophysics Data System (ADS)

Stoica, Eniko T.; Marcauteanu, Corina; Bradu, Adrian; Sinescu, Cosmin; Topala, Florin Ionel; Negrutiu, Meda Lavinia; Duma, Virgil Florin; Podoleanu, Adrian Gh.

2014-01-01

Non-carious cervical lesions (NCCL) are defined as the loss of tooth substance at the cemento-enamel junction and are caused by abrasion, erosion and/or occlusal overload. In this paper we proved that our fast swept source OCT system is a valuable tool to track the evolution of NCCL lesions in time. On several extracted bicuspids, four levels of NCCL were artificially created. After every level of induced lesion, OCT scanning was performed. B scans were acquired and 3D reconstructions were generated. The swept source OCT instrument used in this study has a central wavelength of 1050 nm, a sweeping range of 106 nm (measured at 10 dB), an average output power of 16 mW and a sweeping rate of 100 kHz. A depth resolution determined by the swept source of 12 μm in air was experimentally obtained. NCCL were measured on the B-scans as 2D images and 3D reconstructions (volumes). For quantitative evaluations of volumes, the Image J software was used. By calculating the areas of the amount of lost tissue corresponding to each difference of Bscans, the final volumes of NCCL were obtained. This swept source OCT method allows the dynamic diagnosis of NCCL in time.

Fast and wide tuning wavelength-swept source based on dispersion-tuned fiber optical parametric oscillator.

PubMed

Zhou, Yue; Cheung, Kim K Y; Li, Qin; Yang, Sigang; Chui, P C; Wong, Kenneth K Y

2010-07-15

We demonstrate a dispersion-tuned fiber optical parametric oscillator (FOPO)-based swept source with a sweep rate of 40 kHz and a wavelength tuning range of 109 nm around 1550 nm. The cumulative speed exceeds 4,000,000 nm/s. The FOPO is pumped by a sinusoidally modulated pump, which is driven by a clock sweeping linearly from 1 to 1.0006 GHz. A spool of dispersion-compensating fiber is added inside the cavity to perform dispersion tuning. The instantaneous linewidth is 0.8 nm without the use of any wavelength selective element inside the cavity. 1 GHz pulses with pulse width of 150 ps are generated.

Real-time speckle variance swept-source optical coherence tomography using a graphics processing unit.

PubMed

Lee, Kenneth K C; Mariampillai, Adrian; Yu, Joe X Z; Cadotte, David W; Wilson, Brian C; Standish, Beau A; Yang, Victor X D

2012-07-01

Advances in swept source laser technology continues to increase the imaging speed of swept-source optical coherence tomography (SS-OCT) systems. These fast imaging speeds are ideal for microvascular detection schemes, such as speckle variance (SV), where interframe motion can cause severe imaging artifacts and loss of vascular contrast. However, full utilization of the laser scan speed has been hindered by the computationally intensive signal processing required by SS-OCT and SV calculations. Using a commercial graphics processing unit that has been optimized for parallel data processing, we report a complete high-speed SS-OCT platform capable of real-time data acquisition, processing, display, and saving at 108,000 lines per second. Subpixel image registration of structural images was performed in real-time prior to SV calculations in order to reduce decorrelation from stationary structures induced by the bulk tissue motion. The viability of the system was successfully demonstrated in a high bulk tissue motion scenario of human fingernail root imaging where SV images (512 × 512 pixels, n = 4) were displayed at 54 frames per second.

Swept source optical coherence microscopy using a 1310 nm VCSEL light source

PubMed Central

Ahsen, Osman O.; Tao, Yuankai K.; Potsaid, Benjamin M.; Sheikine, Yuri; Jiang, James; Grulkowski, Ireneusz; Tsai, Tsung-Han; Jayaraman, Vijaysekhar; Kraus, Martin F.; Connolly, James L.; Hornegger, Joachim; Cable, Alex; Fujimoto, James G.

2013-01-01

We demonstrate high speed, swept source optical coherence microscopy (OCM) using a MEMS tunable vertical cavity surface-emitting laser (VCSEL) light source. The light source had a sweep rate of 280 kHz, providing a bidirectional axial scan rate of 560 kHz. The sweep bandwidth was 117 nm centered at 1310 nm, corresponding to an axial resolution of 13.1 µm in air, corresponding to 8.1 µm (9.6 µm spectrally shaped) in tissue. Dispersion mismatch from different objectives was compensated numerically, enabling magnification and field of view to be easily changed. OCM images were acquired with transverse resolutions between 0.86 µm - 3.42 µm using interchangeable 40X, 20X and 10X objectives with ~600 µm x 600 µm, ~1 mm x 1 mm and ~2 mm x 2 mm field-of-view (FOV), respectively. Parasitic variations in path length with beam scanning were corrected numerically. These features enable swept source OCM to be integrated with a wide range of existing scanning microscopes. Large FOV mosaics were generated by serially acquiring adjacent overlapping microscopic fields and combining them in post-processing. Fresh human colon, thyroid and kidney specimens were imaged ex vivo and compared to matching histology sections, demonstrating the ability of OCM to image tissue specimens. PMID:23938673

Ultrahigh phase-stable swept-source optical coherence tomography as a cardiac imaging platform (Conference Presentation)

NASA Astrophysics Data System (ADS)

Ling, Yuye; Hendon, Christine P.

2016-02-01

Functional extensions to optical coherence tomography (OCT) provide useful imaging contrasts that are complementary to conventional OCT. Our goal is to characterize tissue types within the myocardial due to remodeling and therapy. High-speed imaging is necessary to extract mechanical properties and dynamics of fiber orientation changes in a beating heart. Functional extensions of OCT such as polarization sensitive and optical coherence elastography (OCE) require high phase stability of the system, which is a drawback of current mechanically tuned swept source OCT systems. Here we present a high-speed functional imaging platform, which includes an ultrahigh-phase-stable swept source equipped with KTN deflector from NTT-AT. The swept source does not require mechanical movements during the wavelength sweeping; it is electrically tuned. The inter-sweep phase variance of the system was measured to be less than 300 ps at a path length difference of ~2 mm. The axial resolution of the system is 20 µm and the -10 dB fall-off depth is about 3.2 mm. The sample arm has an 8 mmx8 mm field of view with a lateral resolution of approximately 18 µm. The sample arm uses a two-axis MEMS mirror, which is programmable and capable of scanning arbitrary patterns at a sampling rate of 50 kHz. Preliminary imaging results showed differences in polarization properties and image penetration in ablated and normal myocardium. In the future, we will conduct dynamic stretching experiments with strips of human myocardial tissue to characterize mechanical properties using OCE. With high speed imaging of 200 kHz and an all-fiber design, we will work towards catheter-based functional imaging.

ULTRAHIGH SPEED SWEPT SOURCE OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY OF RETINAL AND CHORIOCAPILLARIS ALTERATIONS IN DIABETIC PATIENTS WITH AND WITHOUT RETINOPATHY.

PubMed

Choi, WooJhon; Waheed, Nadia K; Moult, Eric M; Adhi, Mehreen; Lee, ByungKun; De Carlo, Talisa; Jayaraman, Vijaysekhar; Baumal, Caroline R; Duker, Jay S; Fujimoto, James G

2017-01-01

To investigate the utility of ultrahigh speed, swept source optical coherence tomography angiography in visualizing retinal microvascular and choriocapillaris (CC) changes in diabetic patients. The study was prospective and cross-sectional. A 1,050 nm wavelength, 400 kHz A-scan rate swept source optical coherence tomography prototype was used to perform volumetric optical coherence tomography angiography of the retinal and CC vasculatures in diabetic patients and normal subjects. Sixty-three eyes from 32 normal subjects, 9 eyes from 7 patients with proliferative diabetic retinopathy, 29 eyes from 16 patients with nonproliferative diabetic retinopathy, and 51 eyes from 28 diabetic patients without retinopathy were imaged. Retinal and CC microvascular abnormalities were observed in all stages of diabetic retinopathy. In nonproliferative diabetic retinopathy and proliferative diabetic retinopathy, optical coherence tomography angiography visualized a variety of vascular abnormalities, including clustered capillaries, dilated capillary segments, tortuous capillaries, regions of capillary dropout, reduced capillary density, abnormal capillary loops, and foveal avascular zone enlargement. In proliferative diabetic retinopathy, retinal neovascularization above the inner limiting membrane was visualized. Regions of CC flow impairment in patients with proliferative diabetic retinopathy and nonproliferative diabetic retinopathy were also observed. In 18 of the 51 of eyes from diabetic patients without retinopathy, retinal mircrovascular abnormalities were observed and CC flow impairment was found in 24 of the 51 diabetic eyes without retinopathy. The ability of optical coherence tomography angiography to visualize retinal and CC microvascular abnormalities suggests it may be a useful tool for understanding pathogenesis, evaluating treatment response, and earlier detection of vascular abnormalities in patients with diabetes.

SWEPT-SOURCE OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY REVEALS INTERNAL LIMITING MEMBRANE PEELING ALTERS DEEP RETINAL VASCULATURE.

PubMed

Michalewska, Zofia; Nawrocki, Jerzy

2018-04-30

To describe morphology of retinal and choroidal vessels in swept-source optical coherence tomography angiography before and after vitrectomy with the temporal inverted internal limiting membrane (ILM) flap technique for full-thickness macular holes. Prospective, observational study of 36 eyes of 33 patients with full-thickness macular holes swept-source optical coherence tomography angiography was performed in patients before and 1 month after vitrectomy. Vitrectomy with the temporal inverted ILM flap technique was performed. In this method, ILM is peeled only at one side of the fovea. An ILM flap is created to cover the macular hole. Comparison of retina vasculature in the areas of ILM peeling vs. no ILM peeling at 1 and 3 months after successful vitrectomy was performed. The study demonstrated lower density of vessels in the deep retinal plexus in the area where ILM was peeled as compared to the rest of the fovea. Visual acuity and central retinal thickness 1 month after surgery correlates with fovea avascular zone diameter in deep retinal layers at the same time point (P = 0.001). This study confirmed that ILM peeling might alter blood flow in deep retinal vessels below the peeling area in the early postoperative period. The area of the fovea avascular zone corresponds to functional results at the same time point.

Combined in-depth, 3D, en face imaging of the optic disc, optic disc pits and optic disc pit maculopathy using swept-source megahertz OCT at 1050 nm.

PubMed

Maertz, Josef; Kolb, Jan Philip; Klein, Thomas; Mohler, Kathrin J; Eibl, Matthias; Wieser, Wolfgang; Huber, Robert; Priglinger, Siegfried; Wolf, Armin

2018-02-01

To demonstrate papillary imaging of eyes with optic disc pits (ODP) or optic disc pit associated maculopathy (ODP-M) with ultrahigh-speed swept-source optical coherence tomography (SS-OCT) at 1.68 million A-scans/s. To generate 3D-renderings of the papillary area with 3D volume-reconstructions of the ODP and highly resolved en face images from a single densely-sampled megahertz-OCT (MHz-OCT) dataset for investigation of ODP-characteristics. A 1.68 MHz-prototype SS-MHz-OCT system at 1050 nm based on a Fourier-domain mode-locked laser was employed to acquire high-definition, 3D datasets with a dense sampling of 1600 × 1600 A-scans over a 45° field of view. Six eyes with ODPs, and two further eyes with glaucomatous alteration or without ocular pathology are presented. 3D-rendering of the deep papillary structures, virtual 3D-reconstructions of the ODPs and depth resolved isotropic en face images were generated using semiautomatic segmentation. 3D-rendering and en face imaging of the optic disc, ODPs and ODP associated pathologies showed a broad spectrum regarding ODP characteristics. Between individuals the shape of the ODP and the appending pathologies varied considerably. MHz-OCT en face imaging generates distinct top-view images of ODPs and ODP-M. MHz-OCT generates high resolution images of retinal pathologies associated with ODP-M and allows visualizing ODPs with depths of up to 2.7 mm. Different patterns of ODPs can be visualized in patients for the first time using 3D-reconstructions and co-registered high-definition en face images extracted from a single densely sampled 1050 nm megahertz-OCT (MHz-OCT) dataset. As the immediate vicinity to the SAS and the site of intrapapillary proliferation is located at the bottom of the ODP it is crucial to image the complete structure and the whole depth of ODPs. Especially in very deep pits, where non-swept-source OCT fails to reach the bottom, conventional swept-source devices and the MHz-OCT alike are feasible and beneficial methods to examine deep details of optic disc pathologies, while the MHz-OCT bears the advantage of an essentially swifter imaging process.

Multimodal swept-source spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography at 400 kHz

NASA Astrophysics Data System (ADS)

El-Haddad, Mohamed T.; Joos, Karen M.; Patel, Shriji N.; Tao, Yuankai K.

2017-02-01

Multimodal imaging systems that combine scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT) have demonstrated the utility of concurrent en face and volumetric imaging for aiming, eye tracking, bulk motion compensation, mosaicking, and contrast enhancement. However, this additional functionality trades off with increased system complexity and cost because both SLO and OCT generally require dedicated light sources, galvanometer scanners, relay and imaging optics, detectors, and control and digitization electronics. We previously demonstrated multimodal ophthalmic imaging using swept-source spectrally encoded SLO and OCT (SS-SESLO-OCT). Here, we present system enhancements and a new optical design that increase our SS-SESLO-OCT data throughput by >7x and field-of-view (FOV) by >4x. A 200 kHz 1060 nm Axsun swept-source was optically buffered to 400 kHz sweep-rate, and SESLO and OCT were simultaneously digitized on dual input channels of a 4 GS/s digitizer at 1.2 GS/s per channel using a custom k-clock. We show in vivo human imaging of the anterior segment out to the limbus and retinal fundus over a >40° FOV. In addition, nine overlapping volumetric SS-SESLO-OCT volumes were acquired under video-rate SESLO preview and guidance. In post-processing, all nine SESLO images and en face projections of the corresponding OCT volumes were mosaicked to show widefield multimodal fundus imaging with a >80° FOV. Concurrent multimodal SS-SESLO-OCT may have applications in clinical diagnostic imaging by enabling aiming, image registration, and multi-field mosaicking and benefit intraoperative imaging by allowing for real-time surgical feedback, instrument tracking, and overlays of computationally extracted image-based surrogate biomarkers of disease.

Defect inspection of actuator lenses using swept-source optical coherence tomography

NASA Astrophysics Data System (ADS)

Lee, Jaeyul; Shirazi, Muhammad Faizan; Park, Kibeom; Jeon, Mansik; Kim, Jeehyun

2017-12-01

Actuator lens industries have gained an enormous interest with the enhancement of various latest communication devices, such as mobile phone and notebooks. The quality of the aforementioned devices can be degraded due to the internal defects of actuator lenses. Therefore, in this study, we implemented swept-source optical coherence tomography (SS-OCT) system to inspect defects of actuator lenses. Owing to the high-resolution of the SS-OCT system, defected foreign substances between the actuator lenses, defective regions of lenses and surface stains were more clearly distinguished through three-dimensional (3D) and two-dimensional (2D) cross-sectional OCT images. Therefore, the implemented SS-OCT system can be considered as a potential application to defect inspection of actuator lens.

Efficient sweep buffering in swept source optical coherence tomography using a fast optical switch

PubMed Central

Dhalla, Al-Hafeez; Shia, Kevin; Izatt, Joseph A.

2012-01-01

We describe a novel buffering technique for increasing the A-scan rate of swept source optical coherence tomography (SSOCT) systems employing low duty cycle swept source lasers. This technique differs from previously reported buffering techniques in that it employs a fast optical switch, capable of switching in 60 ns, instead of a fused fiber coupler at the end of the buffering stage, and is therefore appreciably more power efficient. The use of the switch also eliminates patient exposure to light that is not used for imaging that occurs at the end of the laser sweep, thereby increasing the system sensitivity. We also describe how careful management of polarization can remove undesirable artifacts due to polarization mode dispersion. In addition, we demonstrate how numerical compensation techniques can be used to modify the signal from a Mach-Zehnder interferometer (MZI) clock obtained from the original sweep to recalibrate the buffered sweep, thereby reducing the complexity of systems employing lasers with integrated MZI clocks. Combining these methods, we constructed an SSOCT system employing an Axsun technologies laser with a sweep rate of 100kHz and 6dB imaging range of 5.5mm. The sweep rate was doubled with sweep buffering to 200 kHz, and the imaging depth was extended to 9 mm using coherence revival. We demonstrated the feasibility of this system by acquiring images of the anterior segments and retinas of healthy human volunteers. PMID:23243559

Efficient sweep buffering in swept source optical coherence tomography using a fast optical switch.

PubMed

Dhalla, Al-Hafeez; Shia, Kevin; Izatt, Joseph A

2012-12-01

We describe a novel buffering technique for increasing the A-scan rate of swept source optical coherence tomography (SSOCT) systems employing low duty cycle swept source lasers. This technique differs from previously reported buffering techniques in that it employs a fast optical switch, capable of switching in 60 ns, instead of a fused fiber coupler at the end of the buffering stage, and is therefore appreciably more power efficient. The use of the switch also eliminates patient exposure to light that is not used for imaging that occurs at the end of the laser sweep, thereby increasing the system sensitivity. We also describe how careful management of polarization can remove undesirable artifacts due to polarization mode dispersion. In addition, we demonstrate how numerical compensation techniques can be used to modify the signal from a Mach-Zehnder interferometer (MZI) clock obtained from the original sweep to recalibrate the buffered sweep, thereby reducing the complexity of systems employing lasers with integrated MZI clocks. Combining these methods, we constructed an SSOCT system employing an Axsun technologies laser with a sweep rate of 100kHz and 6dB imaging range of 5.5mm. The sweep rate was doubled with sweep buffering to 200 kHz, and the imaging depth was extended to 9 mm using coherence revival. We demonstrated the feasibility of this system by acquiring images of the anterior segments and retinas of healthy human volunteers.

Strategies to improve phase-stability of ultrafast swept source optical coherence tomography for single shot imaging of transient mechanical waves at 16 kHz frame rate

DOE Office of Scientific and Technical Information (OSTI.GOV)

Song, Shaozhen; Wei, Wei; Hsieh, Bao-Yu

We present single-shot phase-sensitive imaging of propagating mechanical waves within tissue, enabled by an ultrafast optical coherence tomography (OCT) system powered by a 1.628 MHz Fourier domain mode-locked (FDML) swept laser source. We propose a practical strategy for phase-sensitive measurement by comparing the phases between adjacent OCT B-scans, where the B-scan contains a number of A-scans equaling an integer number of FDML buffers. With this approach, we show that micro-strain fields can be mapped with ∼3.0 nm sensitivity at ∼16 000 fps. The system's capabilities are demonstrated on porcine cornea by imaging mechanical wave propagation launched by a pulsed UV laser beam, promisingmore » non-contact, real-time, and high-resolution optical coherence elastography.« less

Swept-source optical coherence tomography powered by a 1.3-μm vertical cavity surface emitting laser enables 2.3-mm-deep brain imaging in mice in vivo

NASA Astrophysics Data System (ADS)

Choi, Woo June; Wang, Ruikang K.

2015-10-01

We report noninvasive, in vivo optical imaging deep within a mouse brain by swept-source optical coherence tomography (SS-OCT), enabled by a 1.3-μm vertical cavity surface emitting laser (VCSEL). VCSEL SS-OCT offers a constant signal sensitivity of 105 dB throughout an entire depth of 4.25 mm in air, ensuring an extended usable imaging depth range of more than 2 mm in turbid biological tissue. Using this approach, we show deep brain imaging in mice with an open-skull cranial window preparation, revealing intact mouse brain anatomy from the superficial cerebral cortex to the deep hippocampus. VCSEL SS-OCT would be applicable to small animal studies for the investigation of deep tissue compartments in living brains where diseases such as dementia and tumor can take their toll.

TiO2 nanoparticles as exogenous contrast agent for 1 µm swept source optical coherence tomography: an in vitro study

NASA Astrophysics Data System (ADS)

Kumar, Atul; Mondal, Indranil; Roy, Poulomi; Poddar, Raju

2018-03-01

Optical coherence tomography (OCT) is a rapidly evolving, robust technology that has profoundly changed the practice of medical imaging. Swept source OCT (SSOCT) combines the standard time domain and the spatially encoded frequency domain OCT. We have employed a high-speed SSOCT system that utilizes a swept source laser with an A-scan rate of 100 kHz and a central wavelength of 1060 nm for the imaging of the tissue. SSOCT at 1060 nm allows for high penetration in the tissue. TiO2 nanoparticles (NPs) are mostly used for various experimental purposes as an exogenous imaging contrast agent. The in vitro imaging of chicken breast tissue is performed with and without the application of TiO2 NPs for exogenous contrast. Characterization of the chemically synthesized TiO2 NPs was done with dynamic light scattering and a scanning electron microscope method. The effect of TiO2 is studied at different exposure times. A significant improvement in the contrast to noise ratio has been observed through the in vitro imaging of a TiO2 treated tissue.

DETECTION OF TREATMENT-NAIVE CHOROIDAL NEOVASCULARIZATION IN AGE-RELATED MACULAR DEGENERATION BY SWEPT SOURCE OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY.

PubMed

Ahmed, Daniel; Stattin, Martin; Graf, Alexandra; Forster, Julia; Glittenberg, Carl; Krebs, Ilse; Ansari-Shahrezaei, Siamak

2017-09-04

To compare the detection rate of choroidal neovascularization (CNV) in treatment-naive neovascular age-related macular degeneration by swept source optical coherence tomography angiography (SS-OCTA, Topcon's DRI Triton) working at 1,050 nm wavelength versus fluorescence angiography. Cross-sectional analysis of 156 eyes (107 neovascular age-related macular degeneration and 49 dry AMD) in 98 patients, previously diagnosed by multimodal imaging using fluorescein (FA) and indocyanine green angiography (Heidelberg's Spectralis) in a tertiary retina center, evaluated by SS-OCTA 4.5 mm × 4.5 mm and 6 mm × 6 mm macular cubes. Main outcome measures were sensitivity and specificity of SS-OCTA in AMD. Potential factors influencing CNV detection rate were analyzed. Swept source optical coherence tomography angiography detected CNV in 81 of 107 eyes, resulting in a sensitivity of 75.7%. In 49 eyes with dry AMD, no CNV could be identified (specificity 100%). A statistical significance was calculated for nondetection of treatment-naive CNV by SS-OCTA in pigment epithelial detachment over 400 μm (P = 0.0238). Topcon's SS-OCTA was not able to detect all CNV lesions. Large pigment epithelial detachments were associated with signal loss. Fluorescence angiography still remains the gold standard, but the tested SS-OCTA device can be considered as a feasible additional diagnostic tool in AMD.

Robot-assisted three-dimensional registration for cochlear implant surgery using a common-path swept-source optical coherence tomography probe

NASA Astrophysics Data System (ADS)

Gurbani, Saumya S.; Wilkening, Paul; Zhao, Mingtao; Gonenc, Berk; Cheon, Gyeong Woo; Iordachita, Iulian I.; Chien, Wade; Taylor, Russell H.; Niparko, John K.; Kang, Jin U.

2014-05-01

Cochlear implantation offers the potential to restore sensitive hearing in patients with severe to profound deafness. However, surgical placement of the electrode array within the cochlea can produce trauma to sensorineural components, particularly if the initial turn of the cochlea is not successfully navigated as the array is advanced. In this work, we present a robot-mounted common-path swept-source optical coherence tomography endoscopic platform for three-dimensional (3-D) optical coherence tomography (OCT) registration and preoperative surgical planning for cochlear implant surgery. The platform is composed of a common-path 600-μm diameter fiber optic rotary probe attached to a five degrees of freedom robot capable of 1 μm precision movement. The system is tested on a dry fixed ex vivo human temporal bone, and we demonstrate the feasibility of a 3-D OCT registration of the cochlea to accurately describe the spatial and angular profiles of the canal formed by the scala tympani into the first cochlear turn.

Dependence on fiber Fabry-Pérot tunable filter characteristics in an all-fiber swept-wavelength laser for use in an optical coherence tomography system

NASA Astrophysics Data System (ADS)

Stay, Justin L.; Carr, Dustin; Ferguson, Steve; Haber, Todd; Jenkins, Robert; Mock, Joel

2017-02-01

Optical coherence tomography (OCT) has become a useful and common diagnostic tool within the field of ophthalmology. Although presently a commercial technology, research continues in improving image quality and applying the imaging method to other tissue types. Swept-wavelength lasers based upon fiber ring cavities containing fiber Fabry-Ṕerot tunable filters (FFP-TF), as an intracavity element, provide swept-source optical coherence tomography (SS-OCT) systems with a robust and scalable platform. The FFP-TF can be fabricated within a large range of operating wavelengths, free spectral ranges (FSR), and finesses. To date, FFP-TFs have been fabricated at operating wavelengths from 400 nm to 2.2 µm, FSRs as large as 45 THz, and finesses as high as 30 000. The results in this paper focus on presenting the capability of the FFP-TF as an intracavity element in producing swept-wavelength lasers sources and quantifying the trade off between coherence length and sweep range. We present results within a range of feasible operating conditions. Particular focus is given to the discovery of laser configurations that result in maximization of sweep range and/or power. A novel approach to the electronic drive of the PZT-based FFP-TF is also presented, which eliminates the need for the existence of a mechanical resonance of the optical device. This approach substantially increases the range of drive frequencies with which the filter can be driven and has a positive impact for both the short all-fiber laser cavity (presented in this paper) and long cavity FDML designs as well.

Assessing embryo development using swept source optical coherence tomography

NASA Astrophysics Data System (ADS)

Caujolle, S.; Cernat, R.; Silvestri, G.; Marques, M. J.; Bradu, A.; Feuchter, T.; Robinson, G.; Griffin, D.; Podoleanu, A.

2018-03-01

A detailed assessment of embryo development would assist biologists with selecting the most suitable embryos for transfer leading to higher pregnancy rates. Currently, only low resolution microscopy is employed to perform this assessment. Although this method delivers some information on the embryo surface morphology, no specific details are shown related to its inner structure. Using a Master-Slave Swept-Source Optical Coherence Tomography (SS-OCT), images of bovine embryos from day 7 after fertilization were collected from different depths. The dynamic changes inside the embryos were examined, in detail and in real-time from several depths. To prove our ability to characterize the morphology, a single embryo was imaged over 26 hours. The embryo was deprived of its life support environment, leading to its death. Over this period, clear morphological changes were observed.

Wide field and highly sensitive angiography based on optical coherence tomography with akinetic swept source.

PubMed

Xu, Jingjiang; Song, Shaozhen; Wei, Wei; Wang, Ruikang K

2017-01-01

Wide-field vascular visualization in bulk tissue that is of uneven surface is challenging due to the relatively short ranging distance and significant sensitivity fall-off for most current optical coherence tomography angiography (OCTA) systems. We report a long ranging and ultra-wide-field OCTA (UW-OCTA) system based on an akinetic swept laser. The narrow instantaneous linewidth of the swept source with its high phase stability, combined with high-speed detection in the system enable us to achieve long ranging (up to 46 mm) and almost negligible system sensitivity fall-off. To illustrate these advantages, we compare the basic system performances between conventional spectral domain OCTA and UW-OCTA systems and their functional imaging of microvascular networks in living tissues. In addition, we show that the UW-OCTA is capable of different depth-ranging of cerebral blood flow within entire brain in mice, and providing unprecedented blood perfusion map of human finger in vivo . We believe that the UW-OCTA system has promises to augment the existing clinical practice and explore new biomedical applications for OCT imaging.

Wide field and highly sensitive angiography based on optical coherence tomography with akinetic swept source

PubMed Central

Xu, Jingjiang; Song, Shaozhen; Wei, Wei; Wang, Ruikang K.

2016-01-01

Wide-field vascular visualization in bulk tissue that is of uneven surface is challenging due to the relatively short ranging distance and significant sensitivity fall-off for most current optical coherence tomography angiography (OCTA) systems. We report a long ranging and ultra-wide-field OCTA (UW-OCTA) system based on an akinetic swept laser. The narrow instantaneous linewidth of the swept source with its high phase stability, combined with high-speed detection in the system enable us to achieve long ranging (up to 46 mm) and almost negligible system sensitivity fall-off. To illustrate these advantages, we compare the basic system performances between conventional spectral domain OCTA and UW-OCTA systems and their functional imaging of microvascular networks in living tissues. In addition, we show that the UW-OCTA is capable of different depth-ranging of cerebral blood flow within entire brain in mice, and providing unprecedented blood perfusion map of human finger in vivo. We believe that the UW-OCTA system has promises to augment the existing clinical practice and explore new biomedical applications for OCT imaging. PMID:28101428

Tunable Laser Development for In-Flight Fiber Optic Based Structural Health Monitoring Systems

NASA Technical Reports Server (NTRS)

Richards, Lance; Parker, Allen; Chan, Patrick

2013-01-01

Briefing based on tunable laser development for in flight fiber optic based structural health monitoring systems. The objective of this task is to investigate, develop, and demonstrate a low-cost swept lasing light source for NASA DFRC's fiber optics sensing system (FOSS) to perform structural health monitoring on current and future aerospace vehicles.

Suprachoroidal layer and suprachoroidal space delineating the outer margin of the choroid in swept-source optical coherence tomography.

PubMed

Michalewska, Zofia; Michalewski, Janusz; Nawrocka, Zofia; Dulczewska-Cichecka, Karolina; Nawrocki, Jerzy

2015-02-01

To define the morphology of outer choroidal margins in swept-source optical coherence tomography. This is a prospective observational study of 180 eyes: 20 eyes of healthy volunteers, 20 eyes of myopic patients, and 20 eyes from each of the following groups: macular hole, lamellar macular hole, epiretinal membranes, drusen, dry age-related macular degeneration (AMD), neovascular AMD, and vitreomacular traction. A single 12-mm wide swept-source optical coherence tomography image for each of the examined eyes consisting of 1,024 A-scans has been created. The main outcome measure selected was to estimate the presence of suprachoroidal layer, as well as to estimate the ability to delineate the outer choroidoscleral boundary using the software available (DRI-OCT) and to determine its shape. Suprachoroidal layer was observed in 5% of healthy emmetropic eyes, in 50% of eyes with full-thickness macular holes, and in 60% of eyes with vitreomacular traction syndrome. It was also present in 50% of eyes with dry AMD and in 20% of eyes with neovascular AMD. The outer margin of the choroid in all eyes of the healthy volunteers and in eyes with macular diseases has been delineated correctly. In all healthy and myopic eyes, we recognized the outer choroidoscleral boundary as having a regular shape following the natural oval contour of the globe. In eyes with epiretinal membranes, macular hole, vitreomacular traction, and AMD, the outer choroidoscleral boundary was irregular; the choroid varied in thickness from point to point. Swept-source optical coherence tomography enables exact visualization of the outer choroidoscleral boundary. Suprachoroidal layer consisting of two bands has been recognized, the upper of which is hyperreflective and the lower of which is hyporeflective. It may be supposed that the lower hyporeflective band corresponds to suprachoroidal space, which was not earlier visualized in vivo in eyes without choroidal effusion. Suprachoroidal layer in myopic and emmetropic healthy subjects has been rarely observed. We observed it more frequently in different macular diseases.

Corneal topography with high-speed swept source OCT in clinical examination

PubMed Central

Karnowski, Karol; Kaluzny, Bartlomiej J.; Szkulmowski, Maciej; Gora, Michalina; Wojtkowski, Maciej

2011-01-01

We present the applicability of high-speed swept source (SS) optical coherence tomography (OCT) for quantitative evaluation of the corneal topography. A high-speed OCT device of 108,000 lines/s permits dense 3D imaging of the anterior segment within a time period of less than one fourth of second, minimizing the influence of motion artifacts on final images and topographic analysis. The swept laser performance was specially adapted to meet imaging depth requirements. For the first time to our knowledge the results of a quantitative corneal analysis based on SS OCT for clinical pathologies such as keratoconus, a cornea with superficial postinfectious scar, and a cornea 5 months after penetrating keratoplasty are presented. Additionally, a comparison with widely used commercial systems, a Placido-based topographer and a Scheimpflug imaging-based topographer, is demonstrated. PMID:21991558

Tunable Laser Development for In-flight Fiber Optic Based Structural Health Monitoring Systems

NASA Technical Reports Server (NTRS)

Richards, Lance; Parker, Allen; Chan, Patrick

2014-01-01

The objective of this task is to investigate, develop, and demonstrate a low-cost swept lasing light source for NASA DFRC's fiber optics sensing system (FOSS) to perform structural health monitoring on current and future aerospace vehicles. This is the regular update of the Tunable Laser Development for In-flight Fiber Optic Based Structural Health Monitoring Systems website.

In vivo office-based dynamic imaging of vocal cords in awake patients with swept-source optical coherence tomography

NASA Astrophysics Data System (ADS)

Yu, Lingfeng; Liu, Gangjun; Rubinstein, Marc; Saidi, Arya; Guo, Shuguang; Wong, Brian J. F.; Chen, Zhongping

2009-02-01

Optical coherence tomography (OCT) is an evolving noninvasive imaging modality and has been used to image the human larynx during surgical endoscopy. The design of a long GRIN lens based probe capable of capturing images of the human larynx by use of swept-source OCT during a typical office-based laryngoscopy examination is presented. In vivo OCT imaging of the human larynx is demonstrated with 40 fame/second. Dynamic vibration of the vocal folds is recorded to provide not only high-resolution cross-sectional tissue structures but also vibration parameters, such as the vibration frequency and magnitude of the vocal cord, which provide important information for clinical diagnosis and treatment, as well as in fundamental research of the voice. Office-based OCT is a promising imaging modality to study the larynx.

Phase sensitive optical coherence microscopy for photothermal imaging of gold nanorods

NASA Astrophysics Data System (ADS)

Hu, Yong; Podoleanu, Adrian G.; Dobre, George

2018-03-01

We describe a swept source based phase sensitive optical coherence microscopy (OCM) system for photothermal imaging of gold nanorods (GNR). The phase sensitive OCM system employed in the study has a displacement sensitivity of 0.17 nm to vibrations at single frequencies below 250 Hz. We demonstrate the generation of phase maps and confocal phase images. By displaying the difference between successive confocal phase images, we perform the confocal photothermal imaging of accumulated GNRs behind a glass coverslip and behind the scattering media separately. Compared with two-photon luminescence (TPL) detection techniques reported in literature, the technique in this study has the advantage of a simplified experimental setup and provides a more efficient method for imaging the aggregation of GNR. However, the repeatability performance of this technique suffers due to jitter noise from the swept laser source.

Quantitative assessment of rat corneal thickness and morphology during stem cell therapy by high-speed optical coherence tomography

NASA Astrophysics Data System (ADS)

Lal, Cerine; McGrath, James; Subhash, Hrebesh; Rani, Sweta; Ritter, Thomas; Leahy, Martin

2016-03-01

Optical Coherence Tomography (OCT) is a non-invasive 3 dimensional optical imaging modality that enables high resolution cross sectional imaging in biological tissues and materials. Its high axial and lateral resolution combined with high sensitivity, imaging depth and wide field of view makes it suitable for wide variety of high resolution medical imaging applications at clinically relevant speed. With the advent of swept source lasers, the imaging speed of OCT has increased considerably in recent years. OCT has been used in ophthalmology to study dynamic changes occurring in the cornea and iris, thereby providing physiological and pathological changes that occur within the anterior segment structures such as in glaucoma, during refractive surgery, lamellar keratoplasty and corneal diseases. In this study, we assess the changes in corneal thickness in the anterior segment of the eye during wound healing process in a rat corneal burn model following stem cell therapy using high speed swept source OCT.

Volumetric cutaneous microangiography of human skin in vivo by VCSEL swept-source optical coherence tomography

DOE Office of Scientific and Technical Information (OSTI.GOV)

Woo June Choi; Wang, R K

2014-08-31

We demonstrate volumetric cutaneous microangiography of the human skin in vivo that utilises 1.3-μm high-speed sweptsource optical coherence tomography (SS-OCT). The swept source is based on a micro-electro-mechanical (MEMS)-tunable vertical cavity surface emission laser (VCSEL) that is advantageous in terms of long coherence length over 50 mm and 100 nm spectral bandwidth, which enables the visualisation of microstructures within a few mm from the skin surface. We show that the skin microvasculature can be delineated in 3D SS-OCT images using ultrahigh-sensitive optical microangiography (UHS-OMAG) with a correlation mapping mask, providing a contrast enhanced blood perfusion map with capillary flow sensitivity.more » 3D microangiograms of a healthy human finger are shown with distinct cutaneous vessel architectures from different dermal layers and even within hypodermis. These findings suggest that the OCT microangiography could be a beneficial biomedical assay to assess cutaneous vascular functions in clinic. (laser biophotonics)« less

Measuring of the pitch variation of cholesteric liquid crystals under electric field using wavelength-swept laser

NASA Astrophysics Data System (ADS)

Ko, Myeong Ock; Kim, Sung-Jo; Kim, Jong-Hyun; Jeon, Min Yong

2017-04-01

We measure the pitch variation of cholesteric liquid crystals (CLCs) according to the applied electric field using a wavelength-swept laser. While the electric field is applied to the CLC, the pitch of the CLC is elongated normal to the direction of electric field. Therefore, the reflection band is shifted to the longer wavelength. When the applied electric field to the CLC cell was over 1.52 V/μm, the reflection band was changed to the longer wavelength of about 75.1 nm. We believe that the dynamic behavior of the CLC can be analyzed if a high-speed wavelength-swept laser is used as an optical source.

Wide field of view swept-source optical coherence tomography for peripheral retinal disease

PubMed Central

McNabb, Ryan P.; Grewal, Dilraj S.; Mehta, Rajvi; Schuman, Stefanie G.; Izatt, Joseph A.; Mahmoud, Tamer H.; Jaffe, Glenn J.; Mruthyunjaya, Prithvi; Kuo, Anthony N.

2016-01-01

Background/Aims To assess peripheral retinal lesions and the posterior pole in single, widefield optical coherence tomography (OCT) volumes. Methods A wide field of view swept source OCT (WFOV SSOCT) system was developed using a commercial swept source laser and a custom sample arm consisting of two indirect ophthalmic lenses. Twenty-seven subjects with peripheral lesions (choroidal melanomas, choroidal nevii, sclerochoroidal calcification, retinitis pigmentosa, diabetic retinopathy, retinoschisis, and uveitis) were imaged with the WFOV SSOCT. Volumes were taken in primary gaze. Using the optic nerve to fovea distance as a reference measurement, comparisons were made between the lateral field of view (FOV) of the WFOV SSOCT, current generation spectral domain OCT (SDOCT), and widefield scanning laser ophthalmoscopy (SLO) of the same eyes. Results Peripheral pathologies were captured with WFOV SSOCT in 26 of the 27 subjects. The one not captured was in the far nasal periphery and was not seen in the primary gaze volume. Posterior pole associated pathologies were captured in all subjects. Current generation SDOCT had a mean lateral FOV of 2.08 ± 0.21 optic nerve-to-fovea distance units, WFOV SSOCT had a FOV of 4.62 ± 0.62 units, and SLO had a FOV of 9.35 ± 1.02 units. Conclusion WFOV OCT can be used to examine both peripheral retinal pathology and the posterior pole within a single volume acquisition. SLO had the greatest FOV, but does not provide depth information. Future studies using widefield OCT systems will help further delineate the role of WFOV OCT to quantitatively assess and monitor peripheral retinal disease in three dimensions. PMID:26755643

Removing the depth-degeneracy in optical frequency domain imaging with frequency shifting

PubMed Central

Yun, S. H.; Tearney, G. J.; de Boer, J. F.; Bouma, B. E.

2009-01-01

A novel technique using an acousto-optic frequency shifter in optical frequency domain imaging (OFDI) is presented. The frequency shift eliminates the ambiguity between positive and negative differential delays, effectively doubling the interferometric ranging depth while avoiding image cross-talk. A signal processing algorithm is demonstrated to accommodate nonlinearity in the tuning slope of the wavelength-swept OFDI laser source. PMID:19484034

All fiber optics circular-state swept source polarization-sensitive optical coherence tomography.

PubMed

Lin, Hermann; Kao, Meng-Chun; Lai, Chih-Ming; Huang, Jyun-Cin; Kuo, Wen-Chuan

2014-02-01

A swept source (SS)-based circular-state (CS) polarization-sensitive optical coherence tomography (PS-OCT) constructed entirely with polarization-maintaining fiber optics components is proposed with the experimental verification. By means of the proposed calibration scheme, bulk quarter-wave plates can be replaced by fiber optics polarization controllers to, therefore, realize an all-fiber optics CS SSPS-OCT. We also present a numerical dispersion compensation method, which can not only enhance the axial resolution, but also improve the signal-to-noise ratio of the images. We demonstrate that this compact and portable CS SSPS-OCT system with an accuracy comparable to bulk optics systems requires less stringent lens alignment and can possibly serve as a technology to realize PS-OCT instrument for clinical applications (e.g., endoscopy). The largest deviations in the phase retardation (PR) and fast-axis (FA) angle due to sample probe in the linear scanning and a rotation angle smaller than 65 deg were of the same order as those in stationary probe setups. The influence of fiber bending on the measured PR and FA is also investigated. The largest deviations of the PR were 3.5 deg and the measured FA change by ~12 to 21 deg. Finally, in vivo imaging of the human fingertip and nail was successfully demonstrated with a linear scanning probe.

Office-based dynamic imaging of vocal cords in awake patients with swept-source optical coherence tomography

NASA Astrophysics Data System (ADS)

Yu, Lingfeng; Liu, Gangjun; Rubinstein, Marc; Saidi, Arya; Wong, Brian J. F.; Chen, Zhongping

2009-11-01

Optical coherence tomography (OCT) is an evolving noninvasive imaging modality that has been used to image the human larynx during surgical endoscopy. The design of a long gradient index (GRIN) lens-based probe capable of capturing images of the human larynx by use of swept-source OCT during a typical office-based laryngoscopy examination is presented. In vivo OCT imaging of the human larynx is demonstrated with a rate of 40 frames per second. Dynamic vibration of the vocal folds is recorded to provide not only high-resolution cross-sectional tissue structures but also vibration parameters, such as the vibration frequency and magnitude of the vocal cords, which provides important information for clinical diagnosis and treatment, as well as fundamental research of the voice itself. Office-based OCT is a promising imaging modality to study the larynx for physicians in otolaryngology.

Swept source optical coherence tomography of objects with arbitrary reflectivity profiles

NASA Astrophysics Data System (ADS)

Mezgebo, Biniyam; Nagib, Karim; Fernando, Namal; Kordi, Behzad; Sherif, Sherif

2018-03-01

Swept Source optical coherence tomography (SS-OCT) has become a well established imaging modality for both medical and industrial diagnostic applications. A cross-sectional SS-OCT image is obtained by applying an inverse discrete Fourier transform (DFT) to axial interferogram measured in the frequency domain (k-space). Fourier inversion of the obtained interferogram typically produces a potentially overlapping conjugate mirror image, whose overlap could be avoided by restricting the object to have its highest reflectivity at its surface. However, this restriction may not be fulfilled when imaging a very thin object that is placed on a highly reflective surface, or imaging an object containing a contrast agent with high reflectivity. In this paper, we show that oversampling of the SS-OCT signal in k-space would overcome the need for such restriction on the object. Our result is demonstrated using SS-OCT images of Axolotl salamander eggs.

Endoscopic Full-Field Swept-Source Optical Coherence Tomography Neuroimaging System

NASA Astrophysics Data System (ADS)

Felts Almog, Ilan

Optical Coherence Tomography (OCT) has the capability to differentiate brain elements with intrinsic contrast and at a resolution an order-of-magnitude higher than other imaging modalities. This thesis investigates the feasibility of OCT for neuroimaging applied to neurosurgical guidance. We present, to our knowledge, the first Full-Field Swept-Source OCT system operating near a wavelength of 1310 nm, achieving a transverse imaging resolution of 6.5 mum, an axial resolution of 14 mum in tissue and a field of view of 270 mum x 180 mum x 400 mum. Imaging experiments were performed on rat brain tissues ex vivo, human cortical tissue ex vivo, and rats in vivo. A multi-level threshold metric applied on the intensity of the images led to a plausible correlation between the observed density and location in the brain. The proof-of-concept OCT system can be improved and miniaturized for clinical use.

Morphometric measurement of Schlemm's canal in normal human eye using anterior segment swept source optical coherence tomography

NASA Astrophysics Data System (ADS)

Shi, Guohua; Wang, Fei; Li, Xiqi; Lu, Jing; Ding, Zhihua; Sun, Xinghuai; Jiang, Chunhui; Zhang, Yudong

2012-01-01

We have used anterior segment swept source optical coherence tomography to measure Schlemm's canal (SC) morphometric values in the living human eye. Fifty healthy volunteers with 100 normal eyes were measured in the nasal and temporal side. Comparison with the published SC morphometric values of histologic sections proves the reliability of our results. The statistical results show that there are no significant differences between nasal and temporal SC with respect to their diameter, perimeter, and area in our study (diameter: t=0.122, p=0.903; perimeter: t=-0.003, p=0.998; area: t=-1.169, p=0.244); further, no significant differences in SC morphometric values are found between oculus sinister and oculus dexter (diameter: t=0.943, p=0.35; perimeter: t=1.346, p=0.18; area: t=1.501, p=0.135).

Office-based dynamic imaging of vocal cords in awake patients with swept-source optical coherence tomography.

PubMed

Yu, Lingfeng; Liu, Gangjun; Rubinstein, Marc; Saidi, Arya; Wong, Brian J F; Chen, Zhongping

2009-01-01

Optical coherence tomography (OCT) is an evolving noninvasive imaging modality that has been used to image the human larynx during surgical endoscopy. The design of a long gradient index (GRIN) lens-based probe capable of capturing images of the human larynx by use of swept-source OCT during a typical office-based laryngoscopy examination is presented. In vivo OCT imaging of the human larynx is demonstrated with a rate of 40 frames per second. Dynamic vibration of the vocal folds is recorded to provide not only high-resolution cross-sectional tissue structures but also vibration parameters, such as the vibration frequency and magnitude of the vocal cords, which provides important information for clinical diagnosis and treatment, as well as fundamental research of the voice itself. Office-based OCT is a promising imaging modality to study the larynx for physicians in otolaryngology.

Swept-Source Optical Coherence Tomography Angiography in West Nile Virus Chorioretinitis and Associated Occlusive Retinal Vasculitis.

PubMed

Khairallah, Moncef; Kahloun, Rim; Gargouri, Salma; Jelliti, Bechir; Sellami, Dorra; Ben Yahia, Salim; Feki, Jamel

2017-08-01

A 65-year-old man with diabetes and a history of fever of unknown origin 2 weeks earlier complained of sudden decreased vision in the left eye. The patient was diagnosed with bilateral West Nile virus (WNV) chorioretinitis associated with occlusive retinal vasculitis in the left eye. Swept-source optical coherence tomography angiography (SS-OCTA) of the left eye showed extensive, well-delineated, hypointense non-perfusion areas and perifoveal capillary arcade disruption in the superficial capillary plexus, as well as larger non-perfusion areas, capillary rarefaction, and diffuse capillary network attenuation and disorganization in the deep capillary plexus. OCTA may be a valuable tool for noninvasively assessing occlusive retinal vasculitis associated with WNV infection. It allows an accurate detection and precise delineation of areas of retinal capillary nonperfusion in both the superficial and deep capillary plexuses. [Ophthalmic Surg Lasers Imaging Retina. 2017;48:672-675.]. Copyright 2017, SLACK Incorporated.

Office-based dynamic imaging of vocal cords in awake patients with swept-source optical coherence tomography

PubMed Central

Yu, Lingfeng; Liu, Gangjun; Rubinstein, Marc; Saidi, Arya; Wong, Brian J.F.; Chen, Zhongping

2009-01-01

Optical coherence tomography (OCT) is an evolving noninvasive imaging modality that has been used to image the human larynx during surgical endoscopy. The design of a long gradient index (GRIN) lens–based probe capable of capturing images of the human larynx by use of swept-source OCT during a typical office-based laryngoscopy examination is presented. In vivo OCT imaging of the human larynx is demonstrated with a rate of 40 frames per second. Dynamic vibration of the vocal folds is recorded to provide not only high-resolution cross-sectional tissue structures but also vibration parameters, such as the vibration frequency and magnitude of the vocal cords, which provides important information for clinical diagnosis and treatment, as well as fundamental research of the voice itself. Office-based OCT is a promising imaging modality to study the larynx for physicians in otolaryngology. PMID:20059258

LOCATING AND CHARACTERIZING ANGIOID STREAKS WITH EN FACE OPTICAL COHERENCE TOMOGRAPHY.

PubMed

Hanhart, Joel; Greifner, Hillel; Rozenman, Yaakov

2017-01-01

To characterize angioid streaks (AS) with en face optical coherence tomography (OCT). Case report of a patient with myopia presenting with choroidal neovascularization secondary to AS. Swept-source en face OCT ability to image the streaks was compared with spectral-domain and swept-source B-scans as well as color and red-free pictures. A 48-year-old man with myopia presented with sudden central visual loss. Choroidal neovascularization secondary to AS was diagnosed and intraocular anti-vascular endothelial growth factor given with clinical and OCT features improvement. Angioid streaks were visualized as less dark than the overlying retinal and the underlying choroidal vasculature. En face OCT located the changes at the level of Bruch membrane. An AS was found to be interrupted by the choroidal neovascularization, what was not captured by other modalities. En face OCT allows to assess the extent of changes in Bruch membrane and their spatial relationship to choroidal neovascularization.

En face swept-source optical coherence tomographic analysis of X-linked juvenile retinoschisis.

PubMed

Ono, Shinji; Takahashi, Atsushi; Mase, Tomoko; Nagaoka, Taiji; Yoshida, Akitoshi

2016-07-01

To clarify the area of retinoschisis by X-linked juvenile retinoschisis (XLRS) using swept-source optical coherence tomography (SS-OCT) en face images. We report two cases of XLRS in the same family. The patients presented with bilateral blurred vision. The posterior segment examination showed a spoked-wheel pattern in the macula. SS-OCT cross-sectional images revealed widespread retinal splitting at the level of the inner nuclear layer bilaterally. We diagnosed XLRS. To evaluate the area of retinoschisis, we obtained en face SS-OCT images, which clearly visualized the area of retinoschisis seen as a sunflower-like structure in the macula. We report the findings on en face SS-OCT images from patients with XLRS. The en face images using SS-OCT showed the precise area of retinoschisis compared with the SS-OCT thickness map and are useful for managing patients with XLRS.

Development of Extended-Depth Swept Source Optical Coherence Tomography for Applications in Ophthalmic Imaging of the Anterior and Posterior Eye

NASA Astrophysics Data System (ADS)

Dhalla, Al-Hafeez Zahir

Optical coherence tomography (OCT) is a non-invasive optical imaging modality that provides micron-scale resolution of tissue micro-structure over depth ranges of several millimeters. This imaging technique has had a profound effect on the field of ophthalmology, wherein it has become the standard of care for the diagnosis of many retinal pathologies. Applications of OCT in the anterior eye, as well as for imaging of coronary arteries and the gastro-intestinal tract, have also shown promise, but have not yet achieved widespread clinical use. The usable imaging depth of OCT systems is most often limited by one of three factors: optical attenuation, inherent imaging range, or depth-of-focus. The first of these, optical attenuation, stems from the limitation that OCT only detects singly-scattered light. Thus, beyond a certain penetration depth into turbid media, essentially all of the incident light will have been multiply scattered, and can no longer be used for OCT imaging. For many applications (especially retinal imaging), optical attenuation is the most restrictive of the three imaging depth limitations. However, for some applications, especially anterior segment, cardiovascular (catheter-based) and GI (endoscopic) imaging, the usable imaging depth is often not limited by optical attenuation, but rather by the inherent imaging depth of the OCT systems. This inherent imaging depth, which is specific to only Fourier Domain OCT, arises due to two factors: sensitivity fall-off and the complex conjugate ambiguity. Finally, due to the trade-off between lateral resolution and axial depth-of-focus inherent in diffractive optical systems, additional depth limitations sometimes arises in either high lateral resolution or extended depth OCT imaging systems. The depth-of-focus limitation is most apparent in applications such as adaptive optics (AO-) OCT imaging of the retina, and extended depth imaging of the ocular anterior segment. In this dissertation, techniques for extending the imaging range of OCT systems are developed. These techniques include the use of a high spectral purity swept source laser in a full-field OCT system, as well as the use of a peculiar phenomenon known as coherence revival to resolve the complex conjugate ambiguity in swept source OCT. In addition, a technique for extending the depth of focus of OCT systems by using a polarization-encoded, dual-focus sample arm is demonstrated. Along the way, other related advances are also presented, including the development of techniques to reduce crosstalk and speckle artifacts in full-field OCT, and the use of fast optical switches to increase the imaging speed of certain low-duty cycle swept source OCT systems. Finally, the clinical utility of these techniques is demonstrated by combining them to demonstrate high-speed, high resolution, extended-depth imaging of both the anterior and posterior eye simultaneously and in vivo.

Optical frequency switching scheme for a high-speed broadband THz measurement system based on the photomixing technique.

PubMed

Song, Hajun; Hwang, Sejin; Song, Jong-In

2017-05-15

This study presents an optical frequency switching scheme for a high-speed broadband terahertz (THz) measurement system based on the photomixing technique. The proposed system can achieve high-speed broadband THz measurements using narrow optical frequency scanning of a tunable laser source combined with a wavelength-switchable laser source. In addition, this scheme can provide a larger output power of an individual THz signal compared with that of a multi-mode THz signal generated by multiple CW laser sources. A swept-source THz tomography system implemented with a two-channel wavelength-switchable laser source achieves a reduced time for acquisition of a point spread function and a higher depth resolution in the same amount of measurement time compared with a system with a single optical source.

Full-field swept-source optical coherence tomography with phase-shifting techniques for skin cancer detection

NASA Astrophysics Data System (ADS)

Krauter, J.; Boettcher, T.; Körner, K.; Gronle, M.; Osten, W.; Passilly, N.; Froehly, L.; Perrin, S.; Gorecki, C.

2015-05-01

The EU-funded project VIAMOS1 proposes an optical coherence tomography system (OCT) for skin cancer detection, which combines full-field and full-range swept-source OCT in a multi-channel sensor for parallel detection. One of the project objectives is the development of new fabrication technologies for micro-optics, which makes it compatible to Micro-Opto-Electromechanical System technology (MOEMS). The basic system concept is a wafer-based Mirau interferometer array with an actuated reference mirror, which enables phase shifted interferogram detection and therefore reconstruction of the complex phase information, resulting in a higher measurement range with reduced image artifacts. This paper presents an experimental one-channel on-bench OCT system with bulk optics, which serves as a proof-of-concept setup for the final VIAMOS micro-system. It is based on a Linnik interferometer with a wavelength tuning light source and a camera for parallel A-Scan detection. Phase shifting interferometry techniques (PSI) are used for the suppression of the complex conjugate artifact, whose suppression reaches 36 dB. The sensitivity of the system is constant over the full-field with a mean value of 97 dB. OCT images are presented of a thin membrane microlens and a biological tissue (onion) as a preliminary demonstration.

Geometric & radiometric vignetting associated with a 72-facet, off-axis, polygon mirror for swept source optical coherence tomography (SS-OCT)

NASA Astrophysics Data System (ADS)

Everson, Michael; Duma, Virgil-Florin; Dobre, George

2017-01-01

Optical Coherence Tomography (OCT) has a broad range of applications in 2D and volumetric imaging of micron scale structures typically used on inaccessible objects such as the retina of the eye. This report focuses on Swept Source OCT (SS-OCT), favoured for its faster scanning speeds and therefore faster data acquisition (highly favourable when imaging live patients). SS-OCT relies on the scanning of a narrow laser line at speeds typically in excess of 100 kHz. We have employed ZemaxTM ray tracing software to simulate one method of splitting the spectrum of a broadband, near-infrared source, into its component wavelengths by reflecting the spectrum from an off-axis, 72-facet polygon mirror at a frequency of 48 kHz. We specifically addressed the geometric and radiometric vignetting associated with the reflected spectrum off an individual mirrored facet and how this may impose limitations to the incident beam size and hence lead to a loss in the power available from the source. It was found that for certain configurations up to 44% of the light was lost at the edges of the spectrum due to both radiometric and geometric vignetting, which may result in an effective swept range of <50 nm from an initial bandwidth of 100 nm. Our simulations account for real refractive errors and losses in the beam caused by lens aberrations, and produce a model of the sampling function of wavelength against time.

Long ranging swept-source optical coherence tomography-based angiography outperforms its spectral-domain counterpart in imaging human skin microcirculations

NASA Astrophysics Data System (ADS)

Xu, Jingjiang; Song, Shaozhen; Men, Shaojie; Wang, Ruikang K.

2017-11-01

There is an increasing demand for imaging tools in clinical dermatology that can perform in vivo wide-field morphological and functional examination from surface to deep tissue regions at various skin sites of the human body. The conventional spectral-domain optical coherence tomography-based angiography (SD-OCTA) system is difficult to meet these requirements due to its fundamental limitations of the sensitivity roll-off, imaging range as well as imaging speed. To mitigate these issues, we demonstrate a swept-source OCTA (SS-OCTA) system by employing a swept source based on a vertical cavity surface-emitting laser. A series of comparisons between SS-OCTA and SD-OCTA are conducted. Benefiting from the high system sensitivity, long imaging range, and superior roll-off performance, the SS-OCTA system is demonstrated with better performance in imaging human skin than the SD-OCTA system. We show that the SS-OCTA permits remarkable deep visualization of both structure and vasculature (up to ˜2 mm penetration) with wide field of view capability (up to 18×18 mm2), enabling a more comprehensive assessment of the morphological features as well as functional blood vessel networks from the superficial epidermal to deep dermal layers. It is expected that the advantages of the SS-OCTA system will provide a ground for clinical translation, benefiting the existing dermatological practice.

In-line optical fiber metallic mirror reflector for monolithic common path optical coherence tomography probes.

PubMed

Singh, Kanwarpal; Reddy, Rohith; Sharma, Gargi; Verma, Yogesh; Gardecki, Joseph A; Tearney, Guillermo

2018-03-01

Endoscopic optical coherence tomography probes suffer from various artifacts due to dispersion imbalance and polarization mismatch between reference and sample arm light. Such artifacts can be minimized using a common path approach. In this work, we demonstrate a miniaturized common path probe for optical coherence tomography using an inline fiber mirror. A common path optical fiber probe suitable for performing high-resolution endoscopic optical coherence tomography imaging was developed. To achieve common path functionality, an inline fiber mirror was fabricated using a thin gold layer. A commercially available swept source engine was used to test the designed probe in a cadaver human coronary artery ex vivo. We achieved a sensitivity of 104 dB for this probe using a swept source optical coherence tomography system. To test the probe, images of a cadaver human coronary artery were obtained, demonstrating the quality that is comparable to those obtained by OCT systems with separate reference arms. Additionally, we demonstrate recovery of ranging depth by use of a Michelson interferometer in the detection path. We developed a miniaturized monolithic inline fiber mirror-based common path probe for optical coherence tomography. Owing to its simplicity, our design will be helpful in endoscopic applications that require high-resolution probes in a compact form factor while reducing system complexity. Lasers Surg. Med. 50:230-235, 2018. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

In-vivo digital wavefront sensing using swept source OCT

PubMed Central

Kumar, Abhishek; Wurster, Lara M.; Salas, Matthias; Ginner, Laurin; Drexler, Wolfgang; Leitgeb, Rainer A.

2017-01-01

Sub-aperture based digital adaptive optics is demonstrated in a fiber based point scanning optical coherence tomography system using a 1060 nm swept source laser. To detect optical aberrations in-vivo, a small lateral field of view of ~150×150 μm2 is scanned on the sample at a high volume rate of 17 Hz (~1.3 kHz B-scan rate) to avoid any significant lateral and axial motion of the sample, and is used as a “guide star” for the sub-aperture based DAO. The proof of principle is demonstrated using a micro-beads phantom sample, wherein a significant root mean square wavefront error (RMS WFE) of 1.48 waves (> 1μm) is detected. In-vivo aberration measurement with a RMS WFE of 0.33 waves, which is ~5 times higher than the Marechal’s criterion of 1/14 waves for the diffraction limited performance, is shown for a human retinal OCT. Attempt has been made to validate the experimental results with the conventional Shack-Hartmann wavefront sensor within reasonable limitations. PMID:28717573

Silicon photonic integrated circuit swept-source optical coherence tomography receiver with dual polarization, dual balanced, in-phase and quadrature detection.

PubMed

Wang, Zhao; Lee, Hsiang-Chieh; Vermeulen, Diedrik; Chen, Long; Nielsen, Torben; Park, Seo Yeon; Ghaemi, Allan; Swanson, Eric; Doerr, Chris; Fujimoto, James

2015-07-01

Optical coherence tomography (OCT) is a widely used three-dimensional (3D) optical imaging method with many biomedical and non-medical applications. Miniaturization, cost reduction, and increased functionality of OCT systems will be critical for future emerging clinical applications. We present a silicon photonic integrated circuit swept-source OCT (SS-OCT) coherent receiver with dual polarization, dual balanced, in-phase and quadrature (IQ) detection. We demonstrate multiple functional capabilities of IQ polarization resolved detection including: complex-conjugate suppressed full-range OCT, polarization diversity detection, and polarization-sensitive OCT. To our knowledge, this is the first demonstration of a silicon photonic integrated receiver for OCT. The integrated coherent receiver provides a miniaturized, low-cost solution for SS-OCT, and is also a key step towards a fully integrated high speed SS-OCT system with good performance and multi-functional capabilities. With further performance improvement and cost reduction, photonic integrated technology promises to greatly increase penetration of OCT systems in existing applications and enable new applications.

Silicon photonic integrated circuit swept-source optical coherence tomography receiver with dual polarization, dual balanced, in-phase and quadrature detection

PubMed Central

Wang, Zhao; Lee, Hsiang-Chieh; Vermeulen, Diedrik; Chen, Long; Nielsen, Torben; Park, Seo Yeon; Ghaemi, Allan; Swanson, Eric; Doerr, Chris; Fujimoto, James

2015-01-01

Optical coherence tomography (OCT) is a widely used three-dimensional (3D) optical imaging method with many biomedical and non-medical applications. Miniaturization, cost reduction, and increased functionality of OCT systems will be critical for future emerging clinical applications. We present a silicon photonic integrated circuit swept-source OCT (SS-OCT) coherent receiver with dual polarization, dual balanced, in-phase and quadrature (IQ) detection. We demonstrate multiple functional capabilities of IQ polarization resolved detection including: complex-conjugate suppressed full-range OCT, polarization diversity detection, and polarization-sensitive OCT. To our knowledge, this is the first demonstration of a silicon photonic integrated receiver for OCT. The integrated coherent receiver provides a miniaturized, low-cost solution for SS-OCT, and is also a key step towards a fully integrated high speed SS-OCT system with good performance and multi-functional capabilities. With further performance improvement and cost reduction, photonic integrated technology promises to greatly increase penetration of OCT systems in existing applications and enable new applications. PMID:26203382

Sensitivity enhancement in swept-source optical coherence tomography by parametric balanced detector and amplifier

PubMed Central

Kang, Jiqiang; Wei, Xiaoming; Li, Bowen; Wang, Xie; Yu, Luoqin; Tan, Sisi; Jinata, Chandra; Wong, Kenneth K. Y.

2016-01-01

We proposed a sensitivity enhancement method of the interference-based signal detection approach and applied it on a swept-source optical coherence tomography (SS-OCT) system through all-fiber optical parametric amplifier (FOPA) and parametric balanced detector (BD). The parametric BD was realized by combining the signal and phase conjugated idler band that was newly-generated through FOPA, and specifically by superimposing these two bands at a photodetector. The sensitivity enhancement by FOPA and parametric BD in SS-OCT were demonstrated experimentally. The results show that SS-OCT with FOPA and SS-OCT with parametric BD can provide more than 9 dB and 12 dB sensitivity improvement, respectively, when compared with the conventional SS-OCT in a spectral bandwidth spanning over 76 nm. To further verify and elaborate their sensitivity enhancement, a bio-sample imaging experiment was conducted on loach eyes by conventional SS-OCT setup, SS-OCT with FOPA and parametric BD at different illumination power levels. All these results proved that using FOPA and parametric BD could improve the sensitivity significantly in SS-OCT systems. PMID:27446655

Imaging of human finger nail-fold with MHz A-scan rate swept source optical coherence tomography

NASA Astrophysics Data System (ADS)

Poddar, Raju; Mondal, Indranil

2018-07-01

We present a non-invasive three-dimensional depth-resolved micro-structure and micro-vasculature imaging of a human fingernail-fold with a swept-source optical coherence tomography (ssOCT) system at a 1064 nm center wavelength. A phase variance OCT angiography (OCTA) method was implemented for motion contrast OCT imaging. A Fourier-domain mode-locked light source with an A-scan rate of 1.7 MHz (1 700 000 A-scans s‑1) was utilized for imaging. The experimental setup demonstrates OCT and OCTA imaging with an area of ~5 mm  ×  5 mm (within the Nyquist limit). Details of the ssOCTA system such as system parameters, scanning protocols, acquisition time, challenges, and scanning density are discussed. The selected features of the nail-fold structure and vascular networks are also deliberated. The system has potential for real-time monitoring of transdermal drug delivery, and the management and diagnosis of various diseases such as connective tissue diseases and Raynaud’s phenomenon.

Detection of Apical Root Cracks Using Spectral Domain and Swept-source Optical Coherence Tomography.

PubMed

de Oliveira, Bruna Paloma; Câmara, Andréa Cruz; Duarte, Daniel Amancio; Gomes, Anderson Stevens Leonidas; Heck, Richard John; Antonino, Antonio Celso Dantas; Aguiar, Carlos Menezes

2017-07-01

This study aimed to evaluate the ability of 2 optical coherence tomographic (OCT) systems to detect apical dentinal microcracks. Twenty extracted human single-rooted mandibular incisors were selected. After root canal preparation with an R40 Reciproc file (VDW, Munich, Germany), the specimens presenting apical microcracks were identified using micro-computed tomographic (micro-CT) scanning as the gold standard. Then, the apical portions of the roots were imaged with spectral domain OCT (SD-OCT) and swept-source OCT systems, and the resulting images were blindly evaluated by 3 independent examiners to detect microcracks. The diagnostic performance of each OCT device was calculated, and statistical analysis was performed. Based on the micro-CT images, 12 (60%) roots presented dentinal microcracks in the apical region. The images generated by the OCT systems were able to show microcrack lines at the same location as the corresponding micro-CT cross sections. Although the diagnostic performance of the SD-OCT device was superior, there were no statistically significant differences between the 2 OCT devices (P > .05). Interexaminer agreement was substantial to almost perfect for the SD-OCT system and moderate to almost perfect for the swept-source OCT system, whereas intraexaminer agreement was substantial to almost perfect for both OCT devices. The detection ability verified for both OCT systems renders them promising tools for the diagnosis of apical microcracks. Copyright © 2017 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Tuning of successively scanned two monolithic Vernier-tuned lasers and selective data sampling in optical comb swept source optical coherence tomography

PubMed Central

Choi, Dong-hak; Yoshimura, Reiko; Ohbayashi, Kohji

2013-01-01

Monolithic Vernier tuned super-structure grating distributed Bragg reflector (SSG-DBR) lasers are expected to become one of the most promising sources for swept source optical coherence tomography (SS-OCT) with a long coherence length, reduced sensitivity roll-off, and potential capability for a very fast A-scan rate. However, previous implementations of the lasers suffer from four main problems: 1) frequencies deviate from the targeted values when scanned, 2) large amounts of noise appear associated with abrupt changes in injection currents, 3) optically aliased noise appears due to a long coherence length, and 4) the narrow wavelength coverage of a single chip limits resolution. We have developed a method of dynamical frequency tuning, a method of selective data sampling to eliminate current switching noise, an interferometer to reduce aliased noise, and an excess-noise-free connection of two serially scanned lasers to enhance resolution to solve these problems. An optical frequency comb SS-OCT system was achieved with a sensitivity of 124 dB and a dynamic range of 55-72 dB that depended on the depth at an A-scan rate of 3.1 kHz with a resolution of 15 μm by discretely scanning two SSG-DBR lasers, i.e., L-band (1.560-1.599 μm) and UL-band (1.598-1.640 μm). A few OCT images with excellent image penetration depth were obtained. PMID:24409394

Scaled nonuniform Fourier transform for image reconstruction in swept source optical coherence tomography

NASA Astrophysics Data System (ADS)

Mezgebo, Biniyam; Nagib, Karim; Fernando, Namal; Kordi, Behzad; Sherif, Sherif

2018-02-01

Swept Source optical coherence tomography (SS-OCT) is an important imaging modality for both medical and industrial diagnostic applications. A cross-sectional SS-OCT image is obtained by applying an inverse discrete Fourier transform (DFT) to axial interferograms measured in the frequency domain (k-space). This inverse DFT is typically implemented as a fast Fourier transform (FFT) that requires the data samples to be equidistant in k-space. As the frequency of light produced by a typical wavelength-swept laser is nonlinear in time, the recorded interferogram samples will not be uniformly spaced in k-space. Many image reconstruction methods have been proposed to overcome this problem. Most such methods rely on oversampling the measured interferogram then use either hardware, e.g., Mach-Zhender interferometer as a frequency clock module, or software, e.g., interpolation in k-space, to obtain equally spaced samples that are suitable for the FFT. To overcome the problem of nonuniform sampling in k-space without any need for interferogram oversampling, an earlier method demonstrated the use of the nonuniform discrete Fourier transform (NDFT) for image reconstruction in SS-OCT. In this paper, we present a more accurate method for SS-OCT image reconstruction from nonuniform samples in k-space using a scaled nonuniform Fourier transform. The result is demonstrated using SS-OCT images of Axolotl salamander eggs.

Live imaging of rat embryos with Doppler swept-source optical coherence tomography

NASA Astrophysics Data System (ADS)

Larina, Irina V.; Furushima, Kenryo; Dickinson, Mary E.; Behringer, Richard R.; Larin, Kirill V.

2009-09-01

The rat has long been considered an excellent system to study mammalian embryonic cardiovascular physiology, but has lacked the extensive genetic tools available in the mouse to be able to create single gene mutations. However, the recent establishment of rat embryonic stem cell lines facilitates the generation of new models in the rat embryo to link changes in physiology with altered gene function to define the underlying mechanisms behind congenital cardiovascular birth defects. Along with the ability to create new rat genotypes there is a strong need for tools to analyze phenotypes with high spatial and temporal resolution. Doppler OCT has been previously used for 3-D structural analysis and blood flow imaging in other model species. We use Doppler swept-source OCT for live imaging of early postimplantation rat embryos. Structural imaging is used for 3-D reconstruction of embryo morphology and dynamic imaging of the beating heart and vessels, while Doppler-mode imaging is used to visualize blood flow. We demonstrate that Doppler swept-source OCT can provide essential information about the dynamics of early rat embryos and serve as a basis for a wide range of studies on functional evaluation of rat embryo physiology.

Live imaging of rat embryos with Doppler swept-source optical coherence tomography

PubMed Central

Larina, Irina V.; Furushima, Kenryo; Dickinson, Mary E.; Behringer, Richard R.; Larin, Kirill V.

2009-01-01

The rat has long been considered an excellent system to study mammalian embryonic cardiovascular physiology, but has lacked the extensive genetic tools available in the mouse to be able to create single gene mutations. However, the recent establishment of rat embryonic stem cell lines facilitates the generation of new models in the rat embryo to link changes in physiology with altered gene function to define the underlying mechanisms behind congenital cardiovascular birth defects. Along with the ability to create new rat genotypes there is a strong need for tools to analyze phenotypes with high spatial and temporal resolution. Doppler OCT has been previously used for 3-D structural analysis and blood flow imaging in other model species. We use Doppler swept-source OCT for live imaging of early postimplantation rat embryos. Structural imaging is used for 3-D reconstruction of embryo morphology and dynamic imaging of the beating heart and vessels, while Doppler-mode imaging is used to visualize blood flow. We demonstrate that Doppler swept-source OCT can provide essential information about the dynamics of early rat embryos and serve as a basis for a wide range of studies on functional evaluation of rat embryo physiology. PMID:19895102

Ultrahigh speed 1050nm swept source / Fourier domain OCT retinal and anterior segment imaging at 100,000 to 400,000 axial scans per second

PubMed Central

Potsaid, Benjamin; Baumann, Bernhard; Huang, David; Barry, Scott; Cable, Alex E.; Schuman, Joel S.; Duker, Jay S.; Fujimoto, James G.

2011-01-01

We demonstrate ultrahigh speed swept source/Fourier domain ophthalmic OCT imaging using a short cavity swept laser at 100,000–400,000 axial scan rates. Several design configurations illustrate tradeoffs in imaging speed, sensitivity, axial resolution, and imaging depth. Variable rate A/D optical clocking is used to acquire linear-in-k OCT fringe data at 100kHz axial scan rate with 5.3um axial resolution in tissue. Fixed rate sampling at 1 GSPS achieves a 7.5mm imaging range in tissue with 6.0um axial resolution at 100kHz axial scan rate. A 200kHz axial scan rate with 5.3um axial resolution over 4mm imaging range is achieved by buffering the laser sweep. Dual spot OCT using two parallel interferometers achieves 400kHz axial scan rate, almost 2X faster than previous 1050nm ophthalmic results and 20X faster than current commercial instruments. Superior sensitivity roll-off performance is shown. Imaging is demonstrated in the human retina and anterior segment. Wide field 12×12mm data sets include the macula and optic nerve head. Small area, high density imaging shows individual cone photoreceptors. The 7.5mm imaging range configuration can show the cornea, iris, and anterior lens in a single image. These improvements in imaging speed and depth range provide important advantages for ophthalmic imaging. The ability to rapidly acquire 3D-OCT data over a wide field of view promises to simplify examination protocols. The ability to image fine structures can provide detailed information on focal pathologies. The large imaging range and improved image penetration at 1050nm wavelengths promises to improve performance for instrumentation which images both the retina and anterior eye. These advantages suggest that swept source OCT at 1050nm wavelengths will play an important role in future ophthalmic instrumentation. PMID:20940894

Quantitative assessment of oral mucosa and labial minor salivary glands in patients with Sjögren’s syndrome using swept source OCT

PubMed Central

Grulkowski, Ireneusz; Nowak, Jan K.; Karnowski, Karol; Zebryk, Paweł; Puszczewicz, Mariusz; Walkowiak, Jaroslaw; Wojtkowski, Maciej

2013-01-01

Three-dimensional imaging of the mucosa of the lower lip and labial minor salivary glands is demonstrated in vivo using swept source optical coherence tomography (OCT) system at 1310 nm with modified interface. Volumetric data sets of the inner surface of the lower lip covering ~230 mm2 field are obtained from patients with Sjögren’s syndrome and a control group. OCT enables high-resolution visualization of mucosal architecture using cross-sectional images as well as en-face projection images. Comprehensive morphometry of the labial minor salivary glands is performed, and statistical significance is assessed. Statistically significant differences in morphometric parameters are found when subgroups of patients with Sjögren’s syndrome are analyzed. PMID:24466492

Quantitative assessment of oral mucosa and labial minor salivary glands in patients with Sjögren's syndrome using swept source OCT.

PubMed

Grulkowski, Ireneusz; Nowak, Jan K; Karnowski, Karol; Zebryk, Paweł; Puszczewicz, Mariusz; Walkowiak, Jaroslaw; Wojtkowski, Maciej

2013-12-16

Three-dimensional imaging of the mucosa of the lower lip and labial minor salivary glands is demonstrated in vivo using swept source optical coherence tomography (OCT) system at 1310 nm with modified interface. Volumetric data sets of the inner surface of the lower lip covering ~230 mm(2) field are obtained from patients with Sjögren's syndrome and a control group. OCT enables high-resolution visualization of mucosal architecture using cross-sectional images as well as en-face projection images. Comprehensive morphometry of the labial minor salivary glands is performed, and statistical significance is assessed. Statistically significant differences in morphometric parameters are found when subgroups of patients with Sjögren's syndrome are analyzed.

En face projection imaging of the human choroidal layers with tracking SLO and swept source OCT angiography methods

NASA Astrophysics Data System (ADS)

Gorczynska, Iwona; Migacz, Justin; Zawadzki, Robert J.; Sudheendran, Narendran; Jian, Yifan; Tiruveedhula, Pavan K.; Roorda, Austin; Werner, John S.

2015-07-01

We tested and compared the capability of multiple optical coherence tomography (OCT) angiography methods: phase variance, amplitude decorrelation and speckle variance, with application of the split spectrum technique, to image the choroiretinal complex of the human eye. To test the possibility of OCT imaging stability improvement we utilized a real-time tracking scanning laser ophthalmoscopy (TSLO) system combined with a swept source OCT setup. In addition, we implemented a post- processing volume averaging method for improved angiographic image quality and reduction of motion artifacts. The OCT system operated at the central wavelength of 1040nm to enable sufficient depth penetration into the choroid. Imaging was performed in the eyes of healthy volunteers and patients diagnosed with age-related macular degeneration.

Optimisation of a polygon mirror-based spectral filter for swept source optical coherence tomography (SS-OCT)

NASA Astrophysics Data System (ADS)

Everson, Michael; Duma, Virgil-Florin; Dobre, George

2018-03-01

Medical imaging using Optical Coherence Tomography (OCT) provides clinicians with 3D, high resolution reconstructions of microscopic structures, in depth. It has been initially developed for ophthalmology, in order to scan the retinas of patients to diagnose illness. The quality of the images depends upon their axial and lateral resolutions and the properties of the light being used. Research using a polygon mirror (PM) as a spectral filter in Swept Source OCT (SS-OCT) has resulted in a variety of different experimental arrangements. Although the application of PM-based SS-OCT sources has been successfully demonstrated, the combination of their components' fundamental properties and the overall impact they have on imaging performance is rarely reported. A more detailed examination of these properties would lead to a full description of their operation and to the best methods to employ if system performance is to be maximised. This work presents our current findings of on-going research into the optimisation of PM-based SS-OCT systems. A swept source spectral filter, consisting of a collimator, a transmission grating, a two-lens telescope and an off-axis PM with an end reflector mirror has been evaluated experimentally and compared with theoretical predictions. The system's performance has been compared for two different fibre collimators. Although the beam width on the grating is different for each of the two collimators, the spot size at the PM facet is made the same by selecting appropriate focal lengths. An improvement in the signal roll-off at the interferometer output of 1.0 dB/mm was obtained when using a 3.4 mm collimator compared to a 1.5 mm collimator.

Three-dimensional anterior segment imaging in patients with type 1 Boston Keratoprosthesis with switchable full depth range swept source optical coherence tomography

PubMed Central

Poddar, Raju; Cortés, Dennis E.; Werner, John S.; Mannis, Mark J.

2013-01-01

Abstract. A high-speed (100 kHz A-scans/s) complex conjugate resolved 1 μm swept source optical coherence tomography (SS-OCT) system using coherence revival of the light source is suitable for dense three-dimensional (3-D) imaging of the anterior segment. The short acquisition time helps to minimize the influence of motion artifacts. The extended depth range of the SS-OCT system allows topographic analysis of clinically relevant images of the entire depth of the anterior segment of the eye. Patients with the type 1 Boston Keratoprosthesis (KPro) require evaluation of the full anterior segment depth. Current commercially available OCT systems are not suitable for this application due to limited acquisition speed, resolution, and axial imaging range. Moreover, most commonly used research grade and some clinical OCT systems implement a commercially available SS (Axsun) that offers only 3.7 mm imaging range (in air) in its standard configuration. We describe implementation of a common swept laser with built-in k-clock to allow phase stable imaging in both low range and high range, 3.7 and 11.5 mm in air, respectively, without the need to build an external MZI k-clock. As a result, 3-D morphology of the KPro position with respect to the surrounding tissue could be investigated in vivo both at high resolution and with large depth range to achieve noninvasive and precise evaluation of success of the surgical procedure. PMID:23912759

Optic axis determination by fibre-based polarization-sensitive swept-source optical coherence tomography

NASA Astrophysics Data System (ADS)

Lu, Zenghai; Kasaragod, Deepa K.; Matcher, Stephen J.

2011-02-01

We describe a fibre-based variable-incidence angle (VIA) polarization-sensitive swept-source optical coherence tomography (PS-SS-OCT) system to determine the 3D optical axis of birefringent biological tissues. Single-plane VIA-PS-OCT is also explored which requires measurement of the absolute fast-axis orientation. A state-of-the-art PS-SS-OCT system with some improvements both in hardware and software was used to determine the apparent optical birefringence of equine tendon for a number of different illumination directions. Polar and azimuthal angles of cut equine tendon were produced by the VIA method and compared with the nominal values. A quarter waveplate (QWP) and equine tendon were used as test targets to validate the fast-axis measurements using the system. Polar and azimuthal angles of cut equine tendon broadly agreed with the expected values within about 8% of the nominal values. A theoretical and experimental analysis of the effect of the sample arm fibre on determination of optical axis orientation using a proposed definition based on the orientation of the eigenpolarization ellipse experimentally confirms that this algorithm only works correctly for special settings of the sample arm fibre. A proposed algorithm based on the angle between Stokes vectors on the Poincaré sphere is confirmed to work for all settings of the sample arm fibre. A calibration procedure is proposed to remove the sign ambiguity of the measured orientation and was confirmed experimentally by using the QWP.

Optic axis determination by fiber-based polarization-sensitive swept-source optical coherence tomography

NASA Astrophysics Data System (ADS)

Lu, Zenghai; Kasaragod, Deepa K.; Matcher, Stephen J.

2011-03-01

We describe a fiber-based variable-incidence-angle (VIA) polarization-sensitive swept-source optical coherence tomography (PS-SS-OCT) system to determine the 3-D optical axis of birefringent biological tissues. Single-plane VIAPS- OCT is also explored which requires measurement of the absolute fast-axis orientation. A state-of-the-art PS-SS-OCT system with some improvements both in hardware and software was used to determine the apparent optical birefringence of equine tendon for a number of different illumination directions. Polar and azimuthal angles of cut equine tendon were produced by VIA method and compared with the nominal values. A quarter waveplate (QWP) and equine tendon were used as test targets to validate the fast-axis measurements using the system. Polar and azimuthal angles of cut equine tendon broadly agreed with the expected values within about 8% of the nominal values. A theoretical and experimental analysis of the effect of the sample arm fiber on determination of optical axis orientation using a proposed definition based on the orientation of the eigenpolarization ellipse experimentally confirms that this algorithm only works correctly for special settings of the sample arm fiber. A proposed algorithm based on the angle between Stokes vectors on the Poincaré sphere is confirmed to work for all settings of the sample arm fiber. A calibration procedure is proposed to remove the sign ambiguity of the measured orientation and was confirmed experimentally by using the QWP.

Comparison of ocular biometric measurements between a new swept-source optical coherence tomography and a common optical low coherence reflectometry.

PubMed

Gao, Rongrong; Chen, Hao; Savini, Giacomo; Miao, Yaxin; Wang, Xiaorui; Yang, Jing; Zhao, Weiqi; Wang, Qinmei; Huang, Jinhai

2017-05-30

The purpose of the current study was to compare the measurements between a new optical biometer based on swept-source optical coherence tomography (SS-OCT), the OA-2000 (Tomey, Japan), and an optical biometer based on optical low coherence reflectometry (OLCR), the Lenstar (Haag-Streit, Switzerland). Ninety-nine eyes of 99 healthy subjects were included. The axial length (AL), central corneal thickness (CCT), anterior chamber depth (ACD), aqueous depth (AD), lens thickness (LT), keratometry (K) readings, including flat K (Kf), steep K (Ks), mean K (Km), astigmatism vectors J 0 , J 45 at diameters of 2.5 and 3.0 mm, and white-to-white diameter (WTW) were measured three times each using both biometer in normal eyes by random sequence. Bland-Altman analysis showed good agreement between the SS-OCT and OLCR devices for AL, AD, ACD, LT, with narrow 95% LoA (-0.05 to 0.07 mm, -0.09 to 0.10 mm, -0.10 to 0.09 mm, and -0.06 to 0.22 mm, respectively), and the P values of ACD were both >0.05. The CCT, Kf, Ks, Km, J 0 , J 45 and WTW values provided by the OA-2000 were in good agreement with the Lenstar, and statistically significant differences were detected for some of them but not clinical differences. The agreement was excellent especially for AL.

Compensation of spectral artifacts in dual-modality intravascular optical coherence tomography and near-infrared spectroscopy (Conference Presentation)

NASA Astrophysics Data System (ADS)

Fard, Ali M.; Gardecki, Joseph A.; Ughi, Giovanni J.; Hyun, Chulho; Tearney, Guillermo J.

2016-02-01

Intravascular optical coherence tomography (OCT) is a high-resolution catheter-based imaging method that provides three-dimensional microscopic images of coronary artery in vivo, facilitating coronary artery disease treatment decisions based on detailed morphology. Near-infrared spectroscopy (NIRS) has proven to be a powerful tool for identification of lipid-rich plaques inside the coronary walls. We have recently demonstrated a dual-modality intravascular imaging technology that integrates OCT and NIRS into one imaging catheter using a two-fiber arrangement and a custom-made dual-channel fiber rotary junction. It therefore enables simultaneous acquisition of microstructural and composition information at 100 frames/second for improved diagnosis of coronary lesions. The dual-modality OCT-NIRS system employs a single wavelength-swept light source for both OCT and NIRS modalities. It subsequently uses a high-speed photoreceiver to detect the NIRS spectrum in the time domain. Although use of one light source greatly simplifies the system configuration, such light source exhibits pulse-to-pulse wavelength and intensity variation due to mechanical scanning of the wavelength. This can be in particular problematic for NIRS modality and sacrifices the reliability of the acquired spectra. In order to address this challenge, here we developed a robust data acquisition and processing method that compensates for the spectral variations of the wavelength-swept light source. The proposed method extracts the properties of the light source, i.e., variation period and amplitude from a reference spectrum and subsequently calibrates the NIRS datasets. We have applied this method on datasets obtained from cadaver human coronary arteries using a polygon-scanning (1230-1350nm) OCT system, operating at 100,000 sweeps per second. The results suggest that our algorithm accurately and robustly compensates the spectral variations and visualizes the dual-modality OCT-NIRS images. These findings are therefore crucial for the practical application and clinical translation of dual-modality intravascular OCT-NIRS imaging when the same swept sources are used for both OCT and spectroscopy.

Imaging of cardiovascular dynamics in early mouse embryos with swept source optical coherence tomography

NASA Astrophysics Data System (ADS)

Larina, Irina V.; Liebling, Michael; Dickinson, Mary E.; Larin, Kirill V.

2009-02-01

Congenital cardiovascular defects are very common, occurring in 1% of live births, and cardiovascular failures are the leading cause of birth defect-related deaths in infants. To improve diagnostics, prevention and treatment of cardiovascular abnormalities, we need to understand not only how cells form the heart and vessels but also how physical factors such as heart contraction and blood flow influence heart development and changes in the circulatory network. Mouse models are an excellent resource for studying cardiovascular development and disease because of the resemblance to humans, rapid generation time, and availability of mutants with cardiovascular defects linked to human diseases. In this work, we present results on development and application of Doppler Swept Source Optical Coherence Tomography (DSS-OCT) for imaging of cardiovascular dynamics and blood flow in the mouse embryonic heart and vessels. Our studies demonstrated that the spatial and temporal resolution of the DSS-OCT makes it possible to perform sensitive measurements of heart and vessel wall movements and to investigate how contractile waves facilitate the movement of blood through the circulatory system.

Short cavity active mode locking fiber laser for optical sensing and imaging

NASA Astrophysics Data System (ADS)

Lee, Hwi Don; Han, Ga Hee; Jeong, Syung Won; Jeong, Myung Yung; Kim, Chang-Seok; Shin, Jun Geun; Lee, Byeong Ha; Eom, Tae Joong

2014-05-01

We demonstrate a highly linear wavenumber- swept active mode locking (AML) fiber laser for optical sensing and imaging without any wavenumber-space resampling process. In this all-electric AML wavenumber-swept mechanism, a conventional wavelength selection filter is eliminated and, instead, the suitable programmed electric modulation signal is directly applied to the gain medium. Various types of wavenumber (or wavelength) tunings can be implemented because of the filter-less cavity configuration. Therefore, we successfully demonstrate a linearly wavenumber-swept AML fiber laser with 26.5 mW of output power to obtain an in-vivo OCT image at the 100 kHz swept rate.

Frequency comb swept lasers.

PubMed

Tsai, Tsung-Han; Zhou, Chao; Adler, Desmond C; Fujimoto, James G

2009-11-09

We demonstrate a frequency comb (FC) swept laser and a frequency comb Fourier domain mode locked (FC-FDML) laser for applications in optical coherence tomography (OCT). The fiber-based FC swept lasers operate at a sweep rate of 1kHz and 120kHz, respectively over a 135nm tuning range centered at 1310nm with average output powers of 50mW. A 25GHz free spectral range frequency comb filter in the swept lasers causes the lasers to generate a series of well defined frequency steps. The narrow bandwidth (0.015nm) of the frequency comb filter enables a approximately -1.2dB sensitivity roll off over approximately 3mm range, compared to conventional swept source and FDML lasers which have -10dB and -5dB roll offs, respectively. Measurements at very long ranges are possible with minimal sensitivity loss, however reflections from outside the principal measurement range of 0-3mm appear aliased back into the principal range. In addition, the frequency comb output from the lasers are equally spaced in frequency (linear in k-space). The filtered laser output can be used to self-clock the OCT interference signal sampling, enabling direct fast Fourier transformation of the fringe signals, without the need for fringe recalibration procedures. The design and operation principles of FC swept lasers are discussed and designs for short cavity lasers for OCT and interferometric measurement applications are proposed.

Frequency comb swept lasers

PubMed Central

Tsai, Tsung-Han; Zhou, Chao; Adler, Desmond C.; Fujimoto, James G.

2010-01-01

We demonstrate a frequency comb (FC) swept laser and a frequency comb Fourier domain mode locked (FC-FDML) laser for applications in optical coherence tomography (OCT). The fiber-based FC swept lasers operate at a sweep rate of 1kHz and 120kHz, respectively over a 135nm tuning range centered at 1310nm with average output powers of 50mW. A 25GHz free spectral range frequency comb filter in the swept lasers causes the lasers to generate a series of well defined frequency steps. The narrow bandwidth (0.015nm) of the frequency comb filter enables a ~−1.2dB sensitivity roll off over ~3mm range, compared to conventional swept source and FDML lasers which have −10dB and −5dB roll offs, respectively. Measurements at very long ranges are possible with minimal sensitivity loss, however reflections from outside the principal measurement range of 0–3mm appear aliased back into the principal range. In addition, the frequency comb output from the lasers are equally spaced in frequency (linear in k-space). The filtered laser output can be used to self-clock the OCT interference signal sampling, enabling direct fast Fourier transformation of the fringe signals, without the need for fringe recalibration procedures. The design and operation principles of FC swept lasers are discussed and designs for short cavity lasers for OCT and interferometric measurement applications are proposed. PMID:19997365

Optical-domain subsampling for data efficient depth ranging in Fourier-domain optical coherence tomography

PubMed Central

Siddiqui, Meena; Vakoc, Benjamin J.

2012-01-01

Recent advances in optical coherence tomography (OCT) have led to higher-speed sources that support imaging over longer depth ranges. Limitations in the bandwidth of state-of-the-art acquisition electronics, however, prevent adoption of these advances into the clinical applications. Here, we introduce optical-domain subsampling as a method for imaging at high-speeds and over extended depth ranges but with a lower acquisition bandwidth than that required using conventional approaches. Optically subsampled laser sources utilize a discrete set of wavelengths to alias fringe signals along an extended depth range into a bandwidth limited frequency window. By detecting the complex fringe signals and under the assumption of a depth-constrained signal, optical-domain subsampling enables recovery of the depth-resolved scattering signal without overlapping artifacts from this bandwidth-limited window. We highlight key principles behind optical-domain subsampled imaging, and demonstrate this principle experimentally using a polygon-filter based swept-source laser that includes an intra-cavity Fabry-Perot (FP) etalon. PMID:23038343

Four-dimensional Microscope-Integrated Optical Coherence Tomography to Visualize Suture Depth in Strabismus Surgery.

PubMed

Pasricha, Neel D; Bhullar, Paramjit K; Shieh, Christine; Carrasco-Zevallos, Oscar M; Keller, Brenton; Izatt, Joseph A; Toth, Cynthia A; Freedman, Sharon F; Kuo, Anthony N

2017-02-14

The authors report the use of swept-source microscope-integrated optical coherence tomography (SS-MIOCT), capable of live four-dimensional (three-dimensional across time) intraoperative imaging, to directly visualize suture depth during lateral rectus resection. Key surgical steps visualized in this report included needle depth during partial and full-thickness muscle passes along with scleral passes. [J Pediatr Ophthalmol Strabismus. 2017;54:e1-e5.]. Copyright 2017, SLACK Incorporated.

Ultra-compact swept-source optical coherence tomography handheld probe with motorized focus adjustment (Conference Presentation)

NASA Astrophysics Data System (ADS)

LaRocca, Francesco; Nankivil, Derek; Keller, Brenton; Farsiu, Sina; Izatt, Joseph A.

2017-02-01

Handheld optical coherence tomography (OCT) systems facilitate imaging of young children, bedridden subjects, and those with less stable fixation. Smaller and lighter OCT probes allow for more efficient imaging and reduced operator fatigue, which is critical for prolonged use in either the operating room or neonatal intensive care unit. In addition to size and weight, the imaging speed, image quality, field of view, resolution, and focus correction capability are critical parameters that determine the clinical utility of a handheld probe. Here, we describe an ultra-compact swept source (SS) OCT handheld probe weighing only 211 g (half the weight of the next lightest handheld SSOCT probe in the literature) with 20.1 µm lateral resolution, 7 µm axial resolution, 102 dB peak sensitivity, a 27° x 23° field of view, and motorized focus adjustment for refraction correction between -10 to +16 D. A 2D microelectromechanical systems (MEMS) scanner, a converging beam-at-scanner telescope configuration, and an optical design employing 6 different custom optics were used to minimize device size and weight while achieving diffraction limited performance throughout the system's field of view. Custom graphics processing unit (GPU)-accelerated software was used to provide real-time display of OCT B-scans and volumes. Retinal images were acquired from adult volunteers to demonstrate imaging performance.

Optical coherence tomography detection of shear wave propagation in inhomogeneous tissue equivalent phantoms and ex-vivo carotid artery samples

PubMed Central

Razani, Marjan; Luk, Timothy W.H.; Mariampillai, Adrian; Siegler, Peter; Kiehl, Tim-Rasmus; Kolios, Michael C.; Yang, Victor X.D.

2014-01-01

In this work, we explored the potential of measuring shear wave propagation using optical coherence elastography (OCE) in an inhomogeneous phantom and carotid artery samples based on a swept-source optical coherence tomography (OCT) system. Shear waves were generated using a piezoelectric transducer transmitting sine-wave bursts of 400 μs duration, applying acoustic radiation force (ARF) to inhomogeneous phantoms and carotid artery samples, synchronized with a swept-source OCT (SS-OCT) imaging system. The phantoms were composed of gelatin and titanium dioxide whereas the carotid artery samples were embedded in gel. Differential OCT phase maps, measured with and without the ARF, detected the microscopic displacement generated by shear wave propagation in these phantoms and samples of different stiffness. We present the technique for calculating tissue mechanical properties by propagating shear waves in inhomogeneous tissue equivalent phantoms and carotid artery samples using the ARF of an ultrasound transducer, and measuring the shear wave speed and its associated properties in the different layers with OCT phase maps. This method lays the foundation for future in-vitro and in-vivo studies of mechanical property measurements of biological tissues such as vascular tissues, where normal and pathological structures may exhibit significant contrast in the shear modulus. PMID:24688822

Two configurations of miniature Mirau interferometry for swept-source OCT imaging: applications in dermatology and gastroendoscopy

NASA Astrophysics Data System (ADS)

Gorecki, Christophe

2015-08-01

The early diagnosis of cancer is essential since it can be treated more effectively when detected earlier. Visual inspection followed by histological examination is, still today, the gold standard for clinicians. However, a large number of unnecessary surgical procedures are still performed. New diagnostics aids are emerging including the recent techniques of optical coherence tomography (OCT) which permits non-invasive 3D optical biopsies of biological tissues, improving patient's quality of life. Nevertheless, the existing bulk or fiber optics systems are expensive, only affordable at the hospital and thus, not sufficiently used by physicians or cancer's specialists as an early diagnosis tool. We developed two different microsystems based on Mirau interferometry and applied for swept source OCT imaging: one for dermatology and second for gastroenterology. In both cases the architecture is based tem based on spectrally tuned Mirau interferometry. The first configuration, developed in the frame of the European project VIAMOS, includes an active array of 4x4 Mirau interferometers. The matrix of Mirau reference mirrors is integrated on top of an electrostatic vertical comb-drive actuator. In second configuration, developed in the frame of Labex ACTION, we adapted VIAMOS technology to develop an OCT endomicroscope with a single-channel passive Mirau interferometer.

Performance analysis of a full-field and full-range swept-source OCT system

NASA Astrophysics Data System (ADS)

Krauter, J.; Boettcher, T.; Körner, K.; Gronle, M.; Osten, W.; Passilly, N.; Froehly, L.; Perrin, S.; Gorecki, C.

2015-09-01

In recent years, optical coherence tomography (OCT) became gained importance in medical disciplines like ophthalmology, due to its noninvasive optical imaging technique with micrometer resolution and short measurement time. It enables e. g. the measurement and visualization of the depth structure of the retina. In other medical disciplines like dermatology, histopathological analysis is still the gold standard for skin cancer diagnosis. The EU-funded project VIAMOS (Vertically Integrated Array-type Mirau-based OCT System) proposes a new type of OCT system combined with micro-technologies to provide a hand-held, low-cost and miniaturized OCT system. The concept is a combination of full-field and full-range swept-source OCT (SS-OCT) detection in a multi-channel sensor based on a micro-optical Mirau-interferometer array, which is fabricated by means of wafer fabrication. This paper presents the study of an experimental proof-of-concept OCT system as a one-channel sensor with bulk optics. This sensor is a Linnik-interferometer type with similar optical parameters as the Mirau-interferometer array. A commercial wavelength tunable light source with a center wavelength at 845nm and 50nm spectral bandwidth is used with a camera for parallel OCT A-Scan detection. In addition, the reference microscope objective lens of the Linnik-interferometer is mounted on a piezo-actuated phase-shifter. Phase-shifting interferometry (PSI) techniques are applied for resolving the conjugate complex artifact and consequently contribute to an increase of image quality and depth range. A suppression ratio of the complex conjugate term of 36 dB is shown and a system sensitivity greater than 96 dB could be measured.

Real-time calibration-free C-scan images of the eye fundus using Master Slave swept source optical coherence tomography

NASA Astrophysics Data System (ADS)

Bradu, Adrian; Kapinchev, Konstantin; Barnes, Fred; Garway-Heath, David F.; Rajendram, Ranjan; Keane, Pearce; Podoleanu, Adrian G.

2015-03-01

Recently, we introduced a novel Optical Coherence Tomography (OCT) method, termed as Master Slave OCT (MS-OCT), specialized for delivering en-face images. This method uses principles of spectral domain interfereometry in two stages. MS-OCT operates like a time domain OCT, selecting only signals from a chosen depth only while scanning the laser beam across the eye. Time domain OCT allows real time production of an en-face image, although relatively slowly. As a major advance, the Master Slave method allows collection of signals from any number of depths, as required by the user. The tremendous advantage in terms of parallel provision of data from numerous depths could not be fully employed by using multi core processors only. The data processing required to generate images at multiple depths simultaneously is not achievable with commodity multicore processors only. We compare here the major improvement in processing and display, brought about by using graphic cards. We demonstrate images obtained with a swept source at 100 kHz (which determines an acquisition time [Ta] for a frame of 200×200 pixels2 of Ta =1.6 s). By the end of the acquired frame being scanned, using our computing capacity, 4 simultaneous en-face images could be created in T = 0.8 s. We demonstrate that by using graphic cards, 32 en-face images can be displayed in Td 0.3 s. Other faster swept source engines can be used with no difference in terms of Td. With 32 images (or more), volumes can be created for 3D display, using en-face images, as opposed to the current technology where volumes are created using cross section OCT images.

Image-guided feedback for ophthalmic microsurgery using multimodal intraoperative swept-source spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography

NASA Astrophysics Data System (ADS)

Li, Jianwei D.; Malone, Joseph D.; El-Haddad, Mohamed T.; Arquitola, Amber M.; Joos, Karen M.; Patel, Shriji N.; Tao, Yuankai K.

2017-02-01

Surgical interventions for ocular diseases involve manipulations of semi-transparent structures in the eye, but limited visualization of these tissue layers remains a critical barrier to developing novel surgical techniques and improving clinical outcomes. We addressed limitations in image-guided ophthalmic microsurgery by using microscope-integrated multimodal intraoperative swept-source spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography (iSS-SESLO-OCT). We previously demonstrated in vivo human ophthalmic imaging using SS-SESLO-OCT, which enabled simultaneous acquisition of en face SESLO images with every OCT cross-section. Here, we integrated our new 400 kHz iSS-SESLO-OCT, which used a buffered Axsun 1060 nm swept-source, with a surgical microscope and TrueVision stereoscopic viewing system to provide image-based feedback. In vivo human imaging performance was demonstrated on a healthy volunteer, and simulated surgical maneuvers were performed in ex vivo porcine eyes. Denselysampled static volumes and volumes subsampled at 10 volumes-per-second were used to visualize tissue deformations and surgical dynamics during corneal sweeps, compressions, and dissections, and retinal sweeps, compressions, and elevations. En face SESLO images enabled orientation and co-registration with the widefield surgical microscope view while OCT imaging enabled depth-resolved visualization of surgical instrument positions relative to anatomic structures-of-interest. TrueVision heads-up display allowed for side-by-side viewing of the surgical field with SESLO and OCT previews for real-time feedback, and we demonstrated novel integrated segmentation overlays for augmented-reality surgical guidance. Integration of these complementary imaging modalities may benefit surgical outcomes by enabling real-time intraoperative visualization of surgical plans, instrument positions, tissue deformations, and image-based surrogate biomarkers correlated with completion of surgical goals.

Characterization of Long Working Distance Optical Coherence Tomography for Imaging of Pediatric Retinal Pathology.

PubMed

Qian, Ruobing; Carrasco-Zevallos, Oscar M; Mangalesh, Shwetha; Sarin, Neeru; Vajzovic, Lejla; Farsiu, Sina; Izatt, Joseph A; Toth, Cynthia A

2017-10-01

We determined the feasibility of fovea and optic nerve head imaging with a long working distance (LWD) swept source optical coherence tomography (OCT) prototype in adults, teenagers, and young children. A prototype swept source OCT system with a LWD (defined as distance from the last optical element of the imaging system to the eye) of 350 mm with custom fixation targets was developed to facilitate imaging of children. Imaging was performed in 49 participants from three age groups: 26 adults, 16 children 13 to 18 years old (teenagers), and seven children under 6 years old (young children) under an approved institutional review board protocol. The imaging goal was to acquire high quality scans of the fovea and optic nerve in each eye in the shortest time possible. OCT B-scans and volumes of the fovea and optic nerve head of each eligible eye were captured and graded based on four categories (lateral and axial centration, contrast, and resolution) and on ability to determine presence or absence of pathology. LWD-OCT imaging was successful in 88 of 94 eligible eyes, including seven of 10 eyes of young children. Of the successfully acquired OCT images, 83% of B-scan and volumetric images, including 86% from young children, were graded as high-quality scans. Pathology was observed in high-quality OCT images. The prototype LWD-OCT system achieved high quality retinal imaging of adults, teenagers, and some young children with and without pathology with reasonable alignment time. The LWD-OCT system can facilitate imaging in children.

Characterization of Long Working Distance Optical Coherence Tomography for Imaging of Pediatric Retinal Pathology

PubMed Central

Qian, Ruobing; Carrasco-Zevallos, Oscar M.; Mangalesh, Shwetha; Sarin, Neeru; Vajzovic, Lejla; Farsiu, Sina; Izatt, Joseph A.; Toth, Cynthia A.

2017-01-01

Purpose We determined the feasibility of fovea and optic nerve head imaging with a long working distance (LWD) swept source optical coherence tomography (OCT) prototype in adults, teenagers, and young children. Methods A prototype swept source OCT system with a LWD (defined as distance from the last optical element of the imaging system to the eye) of 350 mm with custom fixation targets was developed to facilitate imaging of children. Imaging was performed in 49 participants from three age groups: 26 adults, 16 children 13 to 18 years old (teenagers), and seven children under 6 years old (young children) under an approved institutional review board protocol. The imaging goal was to acquire high quality scans of the fovea and optic nerve in each eye in the shortest time possible. OCT B-scans and volumes of the fovea and optic nerve head of each eligible eye were captured and graded based on four categories (lateral and axial centration, contrast, and resolution) and on ability to determine presence or absence of pathology. Results LWD-OCT imaging was successful in 88 of 94 eligible eyes, including seven of 10 eyes of young children. Of the successfully acquired OCT images, 83% of B-scan and volumetric images, including 86% from young children, were graded as high-quality scans. Pathology was observed in high-quality OCT images. Conclusions The prototype LWD-OCT system achieved high quality retinal imaging of adults, teenagers, and some young children with and without pathology with reasonable alignment time. Translational Relevance The LWD-OCT system can facilitate imaging in children. PMID:29057163

Dental calculus image based on optical coherence tomography

NASA Astrophysics Data System (ADS)

Hsieh, Yao-Sheng; Ho, Yi-Ching; Lee, Shyh-Yuan; Chuang, Ching-Cheng; Wang, Chun-Yang; Sun, Chia-Wei

2011-03-01

In this study, the dental calculus was characterized and imaged by means of swept-source optical coherence tomography (SSOCT). The refractive indices of enamel, dentin, cementum and calculus were measured as 1.625+/-0.024, 1.534+/-0.029, 1.570+/-0.021 and 1.896+/-0.085, respectively. The dental calculus lead strong scattering property and thus the region can be identified under enamel with SSOCT imaging. An extracted human tooth with calculus was covered by gingiva tissue as in vitro sample for SSOCT imaging.

Depth encoded three-beam swept source Doppler optical coherence tomography

NASA Astrophysics Data System (ADS)

Wartak, Andreas; Haindl, Richard; Trasischker, Wolfgang; Baumann, Bernhard; Pircher, Michael; Hitzenberger, Christoph K.

2016-03-01

A novel approach for investigation of human retinal and choroidal blood flow by the means of multi-channel swept source Doppler optical coherence tomography (SS-D-OCT) system is being developed. We present preliminary in vitro measurement results for quantification of the 3D velocity vector of scatterers in a flow phantom. The absolute flow velocity of moving scatterers can be obtained without prior knowledge of flow orientation. In contrast to previous spectral domain (SD-) D-OCT investigations, that already proved the three-channel D-OCT approach to be suitable for in vivo retinal blood flow evaluation, this current work aims for a similar functional approach by means of a differing technique. To the best of our knowledge, this is the first three-channel D-OCT setup featuring a wavelength tunable laser source. Furthermore, we present a modification of our setup allowing a reduction of the former three active illumination channels to one active illumination channel and two passive channels, which only probe the illuminated sample. This joint aperture (JA) approach provides the advantage of not having to divide beam power among three beams to meet corresponding laser safety limits. The in vitro measurement results regarding the flow phantom show good agreement between theoretically calculated and experimentally obtained flow velocity values.

A source-synchronous filter for uncorrelated receiver traces from a swept-frequency seismic source

DOE PAGES

Lord, Neal; Wang, Herbert; Fratta, Dante

2016-09-01

We have developed a novel algorithm to reduce noise in signals obtained from swept-frequency sources by removing out-of-band external noise sources and distortion caused from unwanted harmonics. The algorithm is designed to condition nonstationary signals for which traditional frequency-domain methods for removing noise have been less effective. The source synchronous filter (SSF) is a time-varying narrow band filter, which is synchronized with the frequency of the source signal at all times. Because the bandwidth of the filter needs to account for the source-to-receiver propagation delay and the sweep rate, SSF works best with slow sweep rates and moveout-adjusted waveforms tomore » compensate for source-receiver delays. The SSF algorithm was applied to data collected during a field test at the University of California Santa Barbara’s Garner Valley downhole array site in Southern California. At the site, a 45 kN shaker was mounted on top of a one-story structure and swept from 0 to 10 Hz and back over 60 s (producing useful seismic waves greater than 1.6 Hz). The seismic data were captured with small accelerometer and geophone arrays and with a distributed acoustic sensing array, which is a fiber-optic-based technique for the monitoring of elastic waves. The result of the application of SSF on the field data is a set of undistorted and uncorrelated traces that can be used in different applications, such as measuring phase velocities of surface waves or applying convolution operations with the encoder source function to obtain traveltimes. Lastly, the results from the SSF were used with a visual phase alignment tool to facilitate developing dispersion curves and as a prefilter to improve the interpretation of the data.« less

A source-synchronous filter for uncorrelated receiver traces from a swept-frequency seismic source

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lord, Neal; Wang, Herbert; Fratta, Dante

We have developed a novel algorithm to reduce noise in signals obtained from swept-frequency sources by removing out-of-band external noise sources and distortion caused from unwanted harmonics. The algorithm is designed to condition nonstationary signals for which traditional frequency-domain methods for removing noise have been less effective. The source synchronous filter (SSF) is a time-varying narrow band filter, which is synchronized with the frequency of the source signal at all times. Because the bandwidth of the filter needs to account for the source-to-receiver propagation delay and the sweep rate, SSF works best with slow sweep rates and moveout-adjusted waveforms tomore » compensate for source-receiver delays. The SSF algorithm was applied to data collected during a field test at the University of California Santa Barbara’s Garner Valley downhole array site in Southern California. At the site, a 45 kN shaker was mounted on top of a one-story structure and swept from 0 to 10 Hz and back over 60 s (producing useful seismic waves greater than 1.6 Hz). The seismic data were captured with small accelerometer and geophone arrays and with a distributed acoustic sensing array, which is a fiber-optic-based technique for the monitoring of elastic waves. The result of the application of SSF on the field data is a set of undistorted and uncorrelated traces that can be used in different applications, such as measuring phase velocities of surface waves or applying convolution operations with the encoder source function to obtain traveltimes. Lastly, the results from the SSF were used with a visual phase alignment tool to facilitate developing dispersion curves and as a prefilter to improve the interpretation of the data.« less

Swept optical SSB-SC modulation technique for high-resolution large-dynamic-range static strain measurement using FBG-FP sensors.

PubMed

Huang, Wenzhu; Zhang, Wentao; Li, Fang

2015-04-01

This Letter presents a static strain demodulation technique for FBG-FP sensors using a suppressed carrier LiNbO(3) (LN) optical single sideband (SSB-SC) modulator. A narrow-linewidth tunable laser source is generated by driving the modulator using a linear chirp signal. Then this tunable single-frequency laser is used to interrogate the FBG-FP sensors with the Pound-Drever-Hall (PDH) technique, which is beneficial to eliminate the influence of light intensity fluctuation of the modulator at different tuning frequencies. The static strain is demodulated by calculating the wavelength difference of the PDH signals between the sensing FBG-FP sensor and the reference FBG-FP sensor. As an experimental result using the modulator, the linearity (R²) of the time-frequency response increases from 0.989 to 0.997, and the frequency-swept range (dynamic range) increases from hundreds of MHz to several GHz compared with commercial PZT-tunable lasers. The high-linearity time-wavelength relationship of the modulator is beneficial for improving the strain measurement resolution, as it can solve the problem of the frequency-swept nonlinearity effectively. In the laboratory test, a 0.67 nanostrain static strain resolution, with a 6 GHz dynamic range, is demonstrated.

All-semiconductor high-speed akinetic swept-source for OCT

NASA Astrophysics Data System (ADS)

Minneman, Michael P.; Ensher, Jason; Crawford, Michael; Derickson, Dennis

2011-12-01

A novel swept-wavelength laser for optical coherence tomography (OCT) using a monolithic semiconductor device with no moving parts is presented. The laser is a Vernier-Tuned Distributed Bragg Reflector (VT-DBR) structure exhibiting a single longitudinal mode. All-electronic wavelength tuning is achieved at a 200 kHz sweep repetition rate, 20 mW output power, over 100 nm sweep width and coherence length longer than 40 mm. OCT point-spread functions with 45- 55 dB dynamic range are demonstrated; lasers at 1550 nm, and now 1310 nm, have been developed. Because the laser's long-term tuning stability allows for electronic sample trigger generation at equal k-space intervals (electronic k-clock), the laser does not need an external optical k-clock for measurement interferometer sampling. The non-resonant, allelectronic tuning allows for continuously adjustable sweep repetition rates from mHz to 100s of kHz. Repetition rate duty cycles are continuously adjustable from single-trigger sweeps to over 99% duty cycle. The source includes a monolithically integrated power leveling feature allowing flat or Gaussian power vs. wavelength profiles. Laser fabrication is based on reliable semiconductor wafer-scale processes, leading to low and rapidly decreasing cost of manufacture.

Assessment of cervical demineralization induced by Streptococcus mutans using swept-source optical coherence tomography

PubMed Central

Tezuka, Hiroki; Shimada, Yasushi; Matin, Khairul; Ikeda, Masaomi; Sadr, Alireza; Sumi, Yasunori; Tagami, Junji

2016-01-01

Abstract. Exposed root surfaces due to gingival recession are subject to biofilm stagnation that can result in caries formation. Cervical enamel and dentin demineralization induced by a cariogenic biofilm was evaluated using swept-source optical coherence tomography (SS-OCT). The cementoenamel junction (CEJ) sections of extracted human teeth were subjected to demineralization for 1, 2, or 3 weeks. A suspension of Streptococcus mutans was applied to form a cariogenic biofilm using an oral biofilm reactor. After incubation, demineralization was observed by SS-OCT. For the analysis of SS-OCT signal, the value of the area under the curve (AUC) of the signal profile was measured. Statistical analyses were performed with 95% level of confidence. Cervical demineralization was displayed as a bright zone in SS-OCT. The demineralization depth of dentin was significantly deeper than that of enamel (p<0.05). Enamel near the CEJ demonstrated a significant increase of AUC over the other enamel region after the demineralization. The gaps along the dentinoenamel junction were additionally observed in SS-OCT. SS-OCT was capable of monitoring the cervical demineralization induced by a cariogenic biofilm and is considered to be a promising modality for the diagnosis of cervical demineralization. PMID:27014718

Optical coherence tomography imaging based on non-harmonic analysis

NASA Astrophysics Data System (ADS)

Cao, Xu; Hirobayashi, Shigeki; Chong, Changho; Morosawa, Atsushi; Totsuka, Koki; Suzuki, Takuya

2009-11-01

A new processing technique called Non-Harmonic Analysis (NHA) is proposed for OCT imaging. Conventional Fourier-Domain OCT relies on the FFT calculation which depends on the window function and length. Axial resolution is counter proportional to the frame length of FFT that is limited by the swept range of the swept source in SS-OCT, or the pixel counts of CCD in SD-OCT degraded in FD-OCT. However, NHA process is intrinsically free from this trade-offs; NHA can resolve high frequency without being influenced by window function or frame length of sampled data. In this study, NHA process is explained and applied to OCT imaging and compared with OCT images based on FFT. In order to validate the benefit of NHA in OCT, we carried out OCT imaging based on NHA with the three different sample of onion-skin,human-skin and pig-eye. The results show that NHA process can realize practical image resolution that is equivalent to 100nm swept range only with less than half-reduced wavelength range.

Noninvasive characterisation of foot reflexology areas by swept source-optical coherence tomography in patients with low back pain.

PubMed

Dalal, Krishna; Elanchezhiyan, D; Das, Raunak; Dalal, Devjyoti; Pandey, Ravindra Mohan; Chatterjee, Subhamoy; Upadhyay, Ashish Datt; Maran, V Bharathi; Chatterjee, Jyotirmoy

2013-01-01

Objective. When exploring the scientific basis of reflexology techniques, elucidation of the surface and subsurface features of reflexology areas (RAs) is crucial. In this study, the subcutaneous features of RAs related to the lumbar vertebrae were evaluated by swept source-optical coherence tomography (SS-OCT) in subjects with and without low back pain (LBP). Methods. Volunteers without LBP (n = 6 (male : female = 1 : 1)) and subjects with LBP (n = 15 (male : female = 2 : 3)) were clinically examined in terms of skin colour (visual perception), localised tenderness (visual analogue scale) and structural as well as optical attributes as per SS-OCT. From each subject, 6 optical tomograms were recorded from equidistant transverse planes along the longitudinal axis of the RAs, and from each tomogram, 25 different spatial locations were considered for recording SS-OCT image attributes. The images were analysed with respect to the optical intensity distributions and thicknesses of different skin layers by using AxioVision Rel. 4.8.2 software. The SS-OCT images could be categorised into 4 pathological grades (i.e., 0, 1, 2, and 3) according to distinctness in the visible skin layers. Results. Three specific grades for abnormalities in SS-OCT images were identified considering gradual loss of distinctness and increase in luminosity of skin layers. Almost 90.05% subjects were of mixed type having predominance in certain grades. Conclusion. The skin SS-OCT system demonstrated a definite association of the surface features of healthy/unhealthy RAs with cutaneous features and the clinical status of the lumbar vertebrae.

Noninvasive Characterisation of Foot Reflexology Areas by Swept Source-Optical Coherence Tomography in Patients with Low Back Pain

PubMed Central

Dalal, Krishna; Elanchezhiyan, D.; Das, Raunak; Dalal, Devjyoti; Pandey, Ravindra Mohan; Chatterjee, Subhamoy; Upadhyay, Ashish Datt; Maran, V. Bharathi; Chatterjee, Jyotirmoy

2013-01-01

Objective. When exploring the scientific basis of reflexology techniques, elucidation of the surface and subsurface features of reflexology areas (RAs) is crucial. In this study, the subcutaneous features of RAs related to the lumbar vertebrae were evaluated by swept source-optical coherence tomography (SS-OCT) in subjects with and without low back pain (LBP). Methods. Volunteers without LBP (n = 6 (male : female = 1 : 1)) and subjects with LBP (n = 15 (male : female = 2 : 3)) were clinically examined in terms of skin colour (visual perception), localised tenderness (visual analogue scale) and structural as well as optical attributes as per SS-OCT. From each subject, 6 optical tomograms were recorded from equidistant transverse planes along the longitudinal axis of the RAs, and from each tomogram, 25 different spatial locations were considered for recording SS-OCT image attributes. The images were analysed with respect to the optical intensity distributions and thicknesses of different skin layers by using AxioVision Rel. 4.8.2 software. The SS-OCT images could be categorised into 4 pathological grades (i.e., 0, 1, 2, and 3) according to distinctness in the visible skin layers. Results. Three specific grades for abnormalities in SS-OCT images were identified considering gradual loss of distinctness and increase in luminosity of skin layers. Almost 90.05% subjects were of mixed type having predominance in certain grades. Conclusion. The skin SS-OCT system demonstrated a definite association of the surface features of healthy/unhealthy RAs with cutaneous features and the clinical status of the lumbar vertebrae. PMID:23662156

Anterior chamber angle imaging with swept-source optical coherence tomography: measuring peripheral anterior synechia in glaucoma.

PubMed

Lai, Isabel; Mak, Heather; Lai, Gilda; Yu, Marco; Lam, Dennis S C; Leung, Christopher K S

2013-06-01

To investigate the use of swept-source optical coherence tomography (OCT) for measuring the area and degree of peripheral anterior synechia (PAS) involvement in patients with angle-closure glaucoma. Cross-sectional study. Twenty-three eyes with PAS (detected by indentation gonioscopy) from 20 patients with angle-closure glaucoma (20 eyes had primary angle-closure glaucoma and 3 eyes had angle-closure glaucoma secondary to chronic anterior uveitis [n = 2] and Axenfeld-Rieger syndrome [n = 1]). The anterior chamber angles were evaluated with indentation gonioscopy and imaged by swept-source OCT (Casia OCT, Tomey, Nagoya, Japan) in room light and in the dark using the "angle analysis" protocol, which was composed of 128 radial B-scans each with 512 A-scans (16-mm scan length). The area and degree of PAS involvement were measured in each eye after manual detection of the scleral spur and the anterior irido-angle adhesion by 2 masked observers. The interobserver variability of the PAS measurements was calculated. The agreement of PAS assessment by gonioscopy and OCT, the area and the degree of PAS involvement, and the intraclass correlation coefficient (ICC) of interobserver PAS measurements. The area of PAS (mean ± standard deviation) was 20.8 ± 16.9 mm(2) (range, 3.9-74.9 mm(2)), and the degree of PAS involvement was 186.5 ± 79.9 degrees (range, 42-314 degrees). There was no difference in the area of PAS (P = 0.90) and the degree of PAS involvement (P = 0.95) between images obtained in room light and in the dark. The interobserver ICCs were 0.99 (95% confidence interval [CI], 0.98-1.00) for the area of PAS and 0.99 (95% CI, 0.97-1.00) for the degree of PAS involvement. There was good agreement of PAS assessment between gonioscopy and OCT images (kappa = 0.79; 95% CI, 0.67-0.91). Swept-source OCT allows visualization and reproducible measurements of the area and degree of PAS involvement, providing a new paradigm for evaluation of PAS progression and risk assessment for development of angle-closure glaucoma. The author(s) have no proprietary or commercial interest in any materials discussed in this article. Copyright © 2013 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved.

Study on image feature extraction and classification for human colorectal cancer using optical coherence tomography

NASA Astrophysics Data System (ADS)

Huang, Shu-Wei; Yang, Shan-Yi; Huang, Wei-Cheng; Chiu, Han-Mo; Lu, Chih-Wei

2011-06-01

Most of the colorectal cancer has grown from the adenomatous polyp. Adenomatous lesions have a well-documented relationship to colorectal cancer in previous studies. Thus, to detect the morphological changes between polyp and tumor can allow early diagnosis of colorectal cancer and simultaneous removal of lesions. OCT (Optical coherence tomography) has been several advantages including high resolution and non-invasive cross-sectional image in vivo. In this study, we investigated the relationship between the B-scan OCT image features and histology of malignant human colorectal tissues, also en-face OCT image and the endoscopic image pattern. The in-vitro experiments were performed by a swept-source optical coherence tomography (SS-OCT) system; the swept source has a center wavelength at 1310 nm and 160nm in wavelength scanning range which produced 6 um axial resolution. In the study, the en-face images were reconstructed by integrating the axial values in 3D OCT images. The reconstructed en-face images show the same roundish or gyrus-like pattern with endoscopy images. The pattern of en-face images relate to the stages of colon cancer. Endoscopic OCT technique would provide three-dimensional imaging and rapidly reconstruct en-face images which can increase the speed of colon cancer diagnosis. Our results indicate a great potential for early detection of colorectal adenomas by using the OCT imaging.

Optical analysis of enamel and dentin caries in relation to mineral density using swept-source optical coherence tomography

PubMed Central

Ueno, Tomoka; Shimada, Yasushi; Matin, Khairul; Zhou, Yuan; Wada, Ikumi; Sadr, Alireza; Sumi, Yasunori; Tagami, Junji

2016-01-01

Abstract. The aim of this study was to evaluate the signal intensity and signal attenuation of swept source optical coherence tomography (SS-OCT) for dental caries in relation to the variation of mineral density. SS-OCT observation was performed on the enamel and dentin artificial demineralization and on natural caries. The artificial caries model on enamel and dentin surfaces was created using Streptococcus mutans biofilms incubated in an oral biofilm reactor. The lesions were centrally cross sectioned and SS-OCT scans were obtained in two directions to construct a three-dimensional data set, from the lesion surface (sagittal scan) and parallel to the lesion surface (horizontal scan). The integrated signal up to 200  μm in depth (IS200) and the attenuation coefficient (μ) of the enamel and dentin lesions were calculated from the SS-OCT signal in horizontal scans at five locations of lesion depth. The values were compared with the mineral density obtained from transverse microradiography. Both enamel and dentin demineralization showed significantly higher IS200 and μ than the sound tooth substrate from the sagittal scan. Enamel demineralization showed significantly higher IS200 than sound enamel, even with low levels of demineralization. In demineralized dentin, the μ from the horizontal scan consistently trended downward compared to the sound dentin. PMID:27704033

Evaluation of Retinal and Choroidal Thickness by Swept-Source Optical Coherence Tomography: Repeatability and Assessment of Artifacts

PubMed Central

Mansouri, Kaweh; Medeiros, Felipe A.; Tatham, Andrew J.; Marchase, Nicholas; Weinreb, Robert N.

2017-01-01

PURPOSE To determine the repeatability of automated retinal and choroidal thickness measurements with swept-source optical coherence tomography (SS OCT) and the frequency and type of scan artifacts. DESIGN Prospective evaluation of new diagnostic technology. METHODS Thirty healthy subjects were recruited prospectively and underwent imaging with a prototype SS OCT instrument. Undilated scans of 54 eyes of 27 subjects (mean age, 35.1 ± 9.3 years) were obtained. Each subject had 4 SS OCT protocols repeated 3 times: 3-dimensional (3D) 6 × 6-mm raster scan of the optic disc and macula, radial, and line scan. Automated measurements were obtained through segmentation software. Interscan repeatability was assessed by intraclass correlation coefficients (ICCs). RESULTS ICCs for choroidal measurements were 0.92, 0.98, 0.80, and 0.91, respectively, for 3D macula, 3D optic disc, radial, and line scans. ICCs for retinal measurements were 0.39, 0.49, 0.71, and 0.69, respectively. Artifacts were present in up to 9% scans. Signal loss because of blinking was the most common artifact on 3D scans (optic disc scan, 7%; macula scan, 9%), whereas segmentation failure occurred in 4% of radial and 3% of line scans. When scans with image artifacts were excluded, ICCs for choroidal thickness increased to 0.95, 0.99, 0.87, and 0.93 for 3D macula, 3D optic disc, radial, and line scans, respectively. ICCs for retinal thickness increased to 0.88, 0.83, 0.89, and 0.76, respectively. CONCLUSIONS Improved repeatability of automated choroidal and retinal thickness measurements was found with the SS OCT after correction of scan artifacts. Recognition of scan artifacts is important for correct interpretation of SS OCT measurements. PMID:24531020

Agreement and clinical comparison between a new swept-source optical coherence tomography-based optical biometer and an optical low-coherence reflectometry biometer

PubMed Central

Arriola-Villalobos, P; Almendral-Gómez, J; Garzón, N; Ruiz-Medrano, J; Fernández-Pérez, C; Martínez-de-la-Casa, J M; Díaz-Valle, D

2017-01-01

Purpose To compare measurements taken using a swept-source optical coherence tomography-based optical biometer (IOLmaster 700) and an optical low-coherence reflectometry biometer (Lenstar 900), and to determine the clinical impacts of differences in their measurements on intraocular lens (IOL) power predictions. Methods Eighty eyes of 80 patients scheduled to undergo cataract surgery were examined with both biometers. The measurements made using each device were axial length (AL), central corneal thickness (CCT), aqueous depth (AQD), lens thickness (LT), mean keratometry (MK), white-to-white distance (WTW), and pupil diameter (PD). Holladay 2 and SRK/T formulas were used to calculate IOL power. Differences in measurement between the two biometers were determined using the paired t-test. Agreement was assessed through intraclass correlation coefficients (ICC) and Bland–Altman plots. Results Mean patient age was 76.3±6.8 years (range 59–89). Using the Lenstar, AL and PD could not be measured in 12.5 and 5.25% of eyes, respectively, while IOLMaster 700 took all measurements in all eyes. The variables CCT, AQD, LT, and MK varied significantly between the two biometers. According to ICCs, correlation between measurements made with both devices was excellent except for WTW and PD. Using the SRK/T formula, IOL power prediction based on the data from the two devices were statistically different, but differences were not clinically significant. Conclusions No clinically relevant differences were detected between the biometers in terms of their measurements and IOL power predictions. Using the IOLMaster 700, it was easier to obtain biometric measurements in eyes with less transparent ocular media or longer AL. PMID:27834962

Precise measurement of volume of eccrine sweat gland in mental sweating by optical coherence tomography

NASA Astrophysics Data System (ADS)

Sugawa, Yoshihiko; Fukuda, Akihiro; Ohmi, Masato

2015-04-01

We have demonstrated dynamic analysis of the physiological function of eccrine sweat glands underneath skin surface by optical coherence tomography (OCT). In this paper, we propose a method for extraction of the specific eccrine sweat gland by means of the connected component extraction process and the adaptive threshold method, where the en face OCT images are constructed by the swept-source OCT. In the experiment, we demonstrate precise measurement of the volume of the sweat gland in response to the external stimulus.

Development of novel high-speed en face optical coherence tomography system using KTN optical beam deflector

NASA Astrophysics Data System (ADS)

Ohmi, Masato; Fukuda, Akihiro; Miyazu, Jun; Ueno, Masahiro; Toyoda, Seiji; Kobayashi, Junya

2015-02-01

We developed a novel high-speed en face optical coherence tomography (OCT) system using a KTa1-xNbxO3 (KTN) optical beam deflector. Using the imaging system, fast scanning was performed at 200 kHz by the KTN beam deflector, while slow scanning was performed at 400 Hz by the galvanometer mirror. In a preliminary experiment, we obtained en face OCT images of a human fingerprint at 400 fps. This is the highest speed reported in time-domain en face OCT imaging and is comparable to the speed of swept-source OCT. A 3D-OCT image of a sweat gland was also obtained by our imaging system.

Improved optical axis determination accuracy for fiber-based polarization-sensitive optical coherence tomography

NASA Astrophysics Data System (ADS)

Lu, Zenghai; Matcher, Stephen J.

2013-03-01

We report on a new calibration technique that permits the accurate extraction of sample Jones matrix and hence fast-axis orientation by using fiber-based polarization-sensitive optical coherence tomography (PS-OCT) that is completely based on non polarization maintaining fiber such as SMF-28. In this technique, two quarter waveplates are used to completely specify the parameters of the system fibers in the sample arm so that the Jones matrix of the sample can be determined directly. The device was validated on measurements of a quarter waveplate and an equine tendon sample by a single-mode fiber-based swept-source PS-OCT system.

Depth-encoded all-fiber swept source polarization sensitive OCT

PubMed Central

Wang, Zhao; Lee, Hsiang-Chieh; Ahsen, Osman Oguz; Lee, ByungKun; Choi, WooJhon; Potsaid, Benjamin; Liu, Jonathan; Jayaraman, Vijaysekhar; Cable, Alex; Kraus, Martin F.; Liang, Kaicheng; Hornegger, Joachim; Fujimoto, James G.

2014-01-01

Polarization sensitive optical coherence tomography (PS-OCT) is a functional extension of conventional OCT and can assess depth-resolved tissue birefringence in addition to intensity. Most existing PS-OCT systems are relatively complex and their clinical translation remains difficult. We present a simple and robust all-fiber PS-OCT system based on swept source technology and polarization depth-encoding. Polarization multiplexing was achieved using a polarization maintaining fiber. Polarization sensitive signals were detected using fiber based polarization beam splitters and polarization controllers were used to remove the polarization ambiguity. A simplified post-processing algorithm was proposed for speckle noise reduction relaxing the demand for phase stability. We demonstrated systems design for both ophthalmic and catheter-based PS-OCT. For ophthalmic imaging, we used an optical clock frequency doubling method to extend the imaging range of a commercially available short cavity light source to improve polarization depth-encoding. For catheter based imaging, we demonstrated 200 kHz PS-OCT imaging using a MEMS-tunable vertical cavity surface emitting laser (VCSEL) and a high speed micromotor imaging catheter. The system was demonstrated in human retina, finger and lip imaging, as well as ex vivo swine esophagus and cardiovascular imaging. The all-fiber PS-OCT is easier to implement and maintain compared to previous PS-OCT systems and can be more easily translated to clinical applications due to its robust design. PMID:25401008

Frequency characterization of a swept- and fixed-wavelength external-cavity quantum cascade laser by use of a frequency comb.

PubMed

Knabe, Kevin; Williams, Paul A; Giorgetta, Fabrizio R; Armacost, Chris M; Crivello, Sam; Radunsky, Michael B; Newbury, Nathan R

2012-05-21

The instantaneous optical frequency of an external-cavity quantum cascade laser (QCL) is characterized by comparison to a near-infrared frequency comb. Fluctuations in the instantaneous optical frequency are analyzed to determine the frequency-noise power spectral density for the external-cavity QCL both during fixed-wavelength and swept-wavelength operation. The noise performance of a near-infrared external-cavity diode laser is measured for comparison. In addition to providing basic frequency metrology of external-cavity QCLs, this comb-calibrated swept QCL system can be applied to rapid, precise broadband spectroscopy in the mid-infrared spectral region.

Optical coherence tomography angiography of optic nerve head and parafovea in multiple sclerosis

PubMed Central

Wang, Xiaogang; Jia, Yali; Spain, Rebecca; Potsaid, Benjamin; Liu, Jonathan J; Baumann, Bernhard; Hornegger, Joachim; Fujimoto, James G; Wu, Qiang; Huang, David

2015-01-01

Aims To investigate swept-source optical coherence tomography (OCT) angiography in the optic nerve head (ONH) and parafoveal regions in patients with multiple sclerosis (MS). Methods Fifty-two MS eyes and 21 healthy control (HC) eyes were included. There were two MS subgroups: 38 MS eyes without an optic neuritis (ON) history (MS −ON), and 14 MS eyes with an ON history (MS +ON). The OCT images were captured by high-speed 1050 nm swept-source OCT. The ONH flow index (FI) and parafoveal FI were quantified from OCT angiograms. Results The mean ONH FI was 0.160±0.010 for the HC group, 0.156±0.017 for the MS−ON group, and 0.140±0.020 for the MS+ON group. The ONH FI of the MS+ON group was reduced by 12.5% compared to HC eyes (p=0.004). A higher percentage of MS+ON eyes had abnormal ONH FI compared to HC patients (43% vs 5%, p=0.01). Mean parafoveal FIs were 0.126±0.007, 0.127±0.010, and 0.129±0.005 for the HC, MS−ON, and MS +ON groups, respectively, and did not differ significantly among them. The coefficient of variation (CV) of intravisit repeatability and intervisit reproducibility were 1.03% and 4.53% for ONH FI, and 1.65% and 3.55% for parafoveal FI. Conclusions Based on OCT angiography, the FI measurement is feasible, highly repeatable and reproducible, and it is suitable for clinical measurement of ONH and parafoveal perfusion. The ONH FI may be useful in detecting damage from ON and quantifying its severity. PMID:24831719

GRAFT-VERSUS-HOST DISEASE PANUVEITIS AND BILATERAL SEROUS DETACHMENTS: MULTIMODAL IMAGING ANALYSIS.

PubMed

Jung, Jesse J; Chen, Michael H; Rofagha, Soraya; Lee, Scott S

2017-01-01

To report the multimodal imaging findings and follow-up of a case of graft-versus-host disease-induced bilateral panuveitis and serous retinal detachments after allogenic bone marrow transplant for acute myeloid leukemia. A 75-year-old black man presented with acute decreased vision in both eyes for 1 week. Clinical examination and multimodal imaging, including spectral domain optical coherence tomography, fundus autofluorescence, fluorescein angiography, and swept-source optical coherence tomography angiography (Investigational Device; Carl Zeiss Meditec Inc) were performed. Clinical examination of the patient revealed anterior and posterior inflammation and bilateral serous retinal detachments. Ultra-widefield fundus autofluorescence demonstrated hyperautofluorescence secondary to subretinal fluid; and fluorescein angiography revealed multiple areas of punctate hyperfluorescence, leakage, and staining of the optic discs. Spectral domain and enhanced depth imaging optical coherence tomography demonstrated subretinal fluid, a thickened, undulating retinal pigment epithelium layer, and a thickened choroid in both eyes. En-face swept-source optical coherence tomography angiography did not show any retinal vascular abnormalities but did demonstrate patchy areas of decreased choriocapillaris flow. An extensive systemic infectious and malignancy workup was negative and the patient was treated with high-dose oral prednisone immunosuppression. Subsequent 6-month follow-up demonstrated complete resolution of the inflammation and bilateral serous detachments after completion of the prednisone taper over a 3-month period. Graft-versus-host disease panuveitis and bilateral serous retinal detachments are rare complications of allogenic bone marrow transplant for acute myeloid leukemia and can be diagnosed with clinical and multimodal imaging analysis. This form of autoimmune inflammation may occur after the recovery of T-cell activity within the donor graft targeting the host. Infectious and recurrent malignancy must be ruled out before initiation of immunosuppression, which can affectively treat this form of graft-versus-host disease.

Swept-source anatomic optical coherence elastography of porcine trachea

NASA Astrophysics Data System (ADS)

Bu, Ruofei; Price, Hillel; Mitran, Sorin; Zdanski, Carlton; Oldenburg, Amy L.

2016-02-01

Quantitative endoscopic imaging is at the vanguard of novel techniques in the assessment upper airway obstruction. Anatomic optical coherence tomography (aOCT) has the potential to provide the geometry of the airway lumen with high-resolution and in 4 dimensions. By coupling aOCT with measurements of pressure, optical coherence elastography (OCE) can be performed to characterize airway wall stiffness. This can aid in identifying regions of dynamic collapse as well as informing computational fluid dynamics modeling to aid in surgical decision-making. Toward this end, here we report on an anatomic optical coherence tomography (aOCT) system powered by a wavelength-swept laser source. The system employs a fiber-optic catheter with outer diameter of 0.82 mm deployed via the bore of a commercial, flexible bronchoscope. Helical scans are performed to measure the airway geometry and to quantify the cross-sectional-area (CSA) of the airway. We report on a preliminary validation of aOCT for elastography, in which aOCT-derived CSA was obtained as a function of pressure to estimate airway wall compliance. Experiments performed on a Latex rubber tube resulted in a compliance measurement of 0.68+/-0.02 mm2/cmH2O, with R2=0.98 over the pressure range from 10 to 40 cmH2O. Next, ex vivo porcine trachea was studied, resulting in a measured compliance from 1.06+/-0.12 to 3.34+/-0.44 mm2/cmH2O, (R2>0.81). The linearity of the data confirms the elastic nature of the airway. The compliance values are within the same order-of-magnitude as previous measurements of human upper airways, suggesting that this system is capable of assessing airway wall compliance in future human studies.

Agreement of Anterior Segment Parameters Obtained From Swept-Source Fourier-Domain and Time-Domain Anterior Segment Optical Coherence Tomography.

PubMed

Chansangpetch, Sunee; Nguyen, Anwell; Mora, Marta; Badr, Mai; He, Mingguang; Porco, Travis C; Lin, Shan C

2018-03-01

To assess the interdevice agreement between swept-source Fourier-domain and time-domain anterior segment optical coherence tomography (AS-OCT). Fifty-three eyes from 41 subjects underwent CASIA2 and Visante OCT imaging. One hundred eighty-degree axis images were measured with the built-in two-dimensional analysis software for the swept-source Fourier-domain AS-OCT (CASIA2) and a customized program for the time-domain AS-OCT (Visante OCT). In both devices, we examined the angle opening distance (AOD), trabecular iris space area (TISA), angle recess area (ARA), anterior chamber depth (ACD), anterior chamber width (ACW), and lens vault (LV). Bland-Altman plots and intraclass correlation (ICC) were performed. Orthogonal linear regression assessed any proportional bias. ICC showed strong correlation for LV (0.925) and ACD (0.992) and moderate agreement for ACW (0.801). ICC suggested good agreement for all angle parameters (0.771-0.878) except temporal AOD500 (0.743) and ARA750 (nasal 0.481; temporal 0.481). There was a proportional bias in nasal ARA750 (slope 2.44, 95% confidence interval [CI]: 1.95-3.18), temporal ARA750 (slope 2.57, 95% CI: 2.04-3.40), and nasal TISA500 (slope 1.30, 95% CI: 1.12-1.54). Bland-Altman plots demonstrated in all measured parameters a minimal mean difference between the two devices (-0.089 to 0.063); however, evidence of constant bias was found in nasal AOD250, nasal AOD500, nasal AOD750, nasal ARA750, temporal AOD500, temporal AOD750, temporal ARA750, and ACD. Among the parameters with constant biases, CASIA2 tends to give the larger numbers. Both devices had generally good agreement. However, there were proportional and constant biases in most angle parameters. Thus, it is not recommended that values be used interchangeably.

Field-programmable gate array-controlled sweep velocity-locked laser pulse generator

NASA Astrophysics Data System (ADS)

Chen, Zhen; Hefferman, Gerald; Wei, Tao

2017-05-01

A field-programmable gate array (FPGA)-controlled sweep velocity-locked laser pulse generator (SV-LLPG) design based on an all-digital phase-locked loop (ADPLL) is proposed. A distributed feedback laser with modulated injection current was used as a swept-frequency laser source. An open-loop predistortion modulation waveform was calibrated using a feedback iteration method to initially improve frequency sweep linearity. An ADPLL control system was then implemented using an FPGA to lock the output of a Mach-Zehnder interferometer that was directly proportional to laser sweep velocity to an on-board system clock. Using this system, linearly chirped laser pulses with a sweep bandwidth of 111.16 GHz were demonstrated. Further testing evaluating the sensing utility of the system was conducted. In this test, the SV-LLPG served as the swept laser source of an optical frequency-domain reflectometry system used to interrogate a subterahertz range fiber structure (sub-THz-FS) array. A static strain test was then conducted and linear sensor results were observed.

FPGA-based real-time swept-source OCT systems for B-scan live-streaming or volumetric imaging

NASA Astrophysics Data System (ADS)

Bandi, Vinzenz; Goette, Josef; Jacomet, Marcel; von Niederhäusern, Tim; Bachmann, Adrian H.; Duelk, Marcus

2013-03-01

We have developed a Swept-Source Optical Coherence Tomography (Ss-OCT) system with high-speed, real-time signal processing on a commercially available Data-Acquisition (DAQ) board with a Field-Programmable Gate Array (FPGA). The Ss-OCT system simultaneously acquires OCT and k-clock reference signals at 500MS/s. From the k-clock signal of each A-scan we extract a remap vector for the k-space linearization of the OCT signal. The linear but oversampled interpolation is followed by a 2048-point FFT, additional auxiliary computations, and a data transfer to a host computer for real-time, live-streaming of B-scan or volumetric C-scan OCT visualization. We achieve a 100 kHz A-scan rate by parallelization of our hardware algorithms, which run on standard and affordable, commercially available DAQ boards. Our main development tool for signal analysis as well as for hardware synthesis is MATLAB® with add-on toolboxes and 3rd-party tools.

Comparison of Repeatability and Agreement between Swept-Source Optical Biometry and Dual-Scheimpflug Topography.

PubMed

Jung, Soyeon; Chin, Hee Seung; Kim, Na Rae; Lee, Kang Won; Jung, Ji Won

2017-01-01

To assess the repeatability and agreement of parameters obtained with two biometers and to compare the predictability. Biometry was performed on 101 eyes with cataract using the IOLMaster 700 and the Galilei G6. Three measurements were obtained per eye with each device, and repeatability was evaluated. The axial length (AL), anterior chamber depth (ACD), keratometry (K), white-to-white (WTW) corneal diameter, central corneal thickness (CCT), and lens thickness (LT) were measured and postoperative predictability was compared. Measurements could not be obtained with the IOLMaster 700 in one eye and in seven eyes with the Galilei G6 due to dense cataract. Both the IOLMaster 700 and Galilei G6 showed good repeatability, although the IOLMaster 700 showed better repeatability than the Galilei G6. There were no statistically significant differences in AL, ACD, steepest K, WTW, and LT ( P > 0.050), although flattest K, mean K, and CCT differed ( P < 0.050). The proportion of eyes with an absolute prediction error within 0.5 D was 85.0% for the IOLMaster 700 and was 80.0% for the Galilei G6 based on the SRK/T formula. Two biometers showed high repeatability and relatively good agreements. The swept-source optical biometer demonstrated better repeatability, penetration, and an overall lower prediction error.

Comparison of choroidal thickness using swept-source and spectral-domain optical coherence tomography in normal Indian eyes.

PubMed

Narendran, Siddharth; Manayath, George; Venkatapathy, Narendran

2018-01-01

Choroidal thickness measurements are reported to differ between spectral-domain optical coherence tomography (SD-OCT) and swept-source OCT (SS-OCT). The aim of this study was to assess the comparability of choroidal thickness measurements using SS-OCT and SD-OCT devices among normal participants. This was a prospective study of 31 (62 eyes) normal participants. Choroidal imaging was performed sequentially with the Spectralis OCT (SD-OCT) and the deep range imaging OCT (DRI OCT-1) (SS-OCT) using standardized imaging protocols. The subfoveal choroidal thickness (SFChT) was measured manually by two masked retinal specialists. Paired t -tests and intraclass correlation coefficients (ICCs) were used to compare the measurements. The mean SFChT was 319.5 μm and 325.3 μm for DRI OCT-1 and Spectralis OCT, respectively ( P = 0.001), with a mean difference of 5.9 with ICC of 0.97. The mean difference in choroidal thickness between the OCT devices was larger among eyes with choroidal thickness > 350 μm compared with eyes with thinner choroids (8.0 μm vs. 4.7 μm). SFChT measurements are comparable between DRI OCT-1 and Spectralis OCT. The variability between the devices increases in thicker choroids.

Detecting apoptosis using dynamic light scattering with optical coherence tomography

NASA Astrophysics Data System (ADS)

Farhat, Golnaz; Mariampillai, Adrian; Yang, Victor X. D.; Czarnota, Gregory J.; Kolios, Michael C.

2011-07-01

A dynamic light scattering technique is implemented using optical coherence tomography (OCT) to measure the change in intracellular motion as cells undergo apoptosis. Acute myeloid leukemia cells were treated with cisplatin and imaged at a frame rate of 166 Hz using a 1300 nm swept-source OCT system at various times over a period of 48 h. Time correlation analysis of the speckle intensities indicated a significant increase in intracellular motion 24 h after treatment. This rise in intracellular motion correlated with histological findings of irregularly shaped and fragmented cells indicative of cell membrane blebbing and fragmentation.

Monitoring the gingival regeneration after aesthetic surgery with optical coherence tomography

NASA Astrophysics Data System (ADS)

Fernandes, Luana O.; Graça, Natalia D. R. L.; Melo, Luciana S. A.; Silva, Claudio H. V.; Gomes, Anderson S. L.

2016-02-01

The aim of this study was to use the Optical Coherence Tomography (OCT) technique working in spectral domain (Swept Source OCT at 1325 nm, Thorlabs, New Jersey, USA) to monitor the tissue repair in patients undergoing periodontal plastic surgery. The evaluations were done over a period of 60 days. It was observed that 15 days after periodontal surgery the gum was still in different healing process as compared to the observation after 60 days. Thus it is clear that, despite some technical limitations, the OCT is an efficient method in the evaluation of regeneration gingival.

Quantitative optical coherence microscopy for the in situ investigation of the biofilm

NASA Astrophysics Data System (ADS)

Meleppat, Ratheesh Kumar; Shearwood, Christopher; Keey, Seah Leong; Matham, Murukeshan Vadakke

2016-12-01

This paper explores the potential of optical coherence microscopy (OCM) for the in situ monitoring of biofilm growth. The quantitative imaging of the early developmental biology of a representative biofilm, Klebsiella pneumonia (KP-1), was performed using a swept source-based Fourier domain OCM system. The growth dynamics of the KP-1 biofilms and their transient response under perturbation was investigated using the enface visualization of microcolonies and their spatial localization. Furthermore, the optical density (OD) and planar density of the biofilms are calculated using an OCM technique and compared with OD and colony forming units measured using standard procedures via the sampling of the flow-cell effluent.

Subaperture correlation based digital adaptive optics for full field optical coherence tomography.

PubMed

Kumar, Abhishek; Drexler, Wolfgang; Leitgeb, Rainer A

2013-05-06

This paper proposes a sub-aperture correlation based numerical phase correction method for interferometric full field imaging systems provided the complex object field information can be extracted. This method corrects for the wavefront aberration at the pupil/ Fourier transform plane without the need of any adaptive optics, spatial light modulators (SLM) and additional cameras. We show that this method does not require the knowledge of any system parameters. In the simulation study, we consider a full field swept source OCT (FF SSOCT) system to show the working principle of the algorithm. Experimental results are presented for a technical and biological sample to demonstrate the proof of the principle.

Microvascular changes during acne lesion initiation and scarring is revealed in vivo using optical microangiography

NASA Astrophysics Data System (ADS)

Baran, Utku; Li, Yuandong; Choi, Woo J.; Wang, Ruikang K.

2015-02-01

Acne is a common skin disease in society and often leads to scarring. In this paper, we demonstrate the capabilities of swept-source optical coherence tomography (SS-OCT) in detecting specific features of acne lesion initiation and scarring on human facial skin in vivo over 30 days. Optical microangiography (OMAG) technique made it possible to image 3D tissue microvasculature changes up to 1 mm depth in vivo without the need of exogenous contrast agents in ~10 seconds. The presented results show promise to facilitate clinical trials of treatment and prognosis of acne vulgaris by detecting cutaneous microvasculature and structural changes within human skin in vivo.

Wavelength shifting of intra-cavity photons: Adiabatic wavelength tuning in rapidly wavelength-swept lasers

PubMed Central

Jirauschek, Christian; Huber, Robert

2015-01-01

We analyze the physics behind the newest generation of rapidly wavelength tunable sources for optical coherence tomography (OCT), retaining a single longitudinal cavity mode during operation without repeated build up of lasing. In this context, we theoretically investigate the currently existing concepts of rapidly wavelength-swept lasers based on tuning of the cavity length or refractive index, leading to an altered optical path length inside the resonator. Specifically, we consider vertical-cavity surface-emitting lasers (VCSELs) with microelectromechanical system (MEMS) mirrors as well as Fourier domain mode-locked (FDML) and Vernier-tuned distributed Bragg reflector (VT-DBR) lasers. Based on heuristic arguments and exact analytical solutions of Maxwell’s equations for a fundamental laser resonator model, we show that adiabatic wavelength tuning is achieved, i.e., hopping between cavity modes associated with a repeated build up of lasing is avoided, and the photon number is conserved. As a consequence, no fundamental limit exists for the wavelength tuning speed, in principle enabling wide-range wavelength sweeps at arbitrary tuning speeds with narrow instantaneous linewidth. PMID:26203373

Ultrahigh speed endoscopic swept source optical coherence tomography using a VCSEL light source and micromotor catheter

NASA Astrophysics Data System (ADS)

Tsai, Tsung-Han; Ahsen, Osman O.; Lee, Hsiang-Chieh; Liang, Kaicheng; Giacomelli, Michael G.; Potsaid, Benjamin M.; Tao, Yuankai K.; Jayaraman, Vijaysekhar; Kraus, Martin F.; Hornegger, Joachim; Figueiredo, Marisa; Huang, Qin; Mashimo, Hiroshi; Cable, Alex E.; Fujimoto, James G.

2014-03-01

We developed an ultrahigh speed endoscopic swept source optical coherence tomography (OCT) system for clinical gastroenterology using a vertical-cavity surface-emitting laser (VCSEL) and micromotor based imaging catheter, which provided an imaging speed of 600 kHz axial scan rate and 8 μm axial resolution in tissue. The micromotor catheter was 3.2 mm in diameter and could be introduced through the 3.7 mm accessory port of an endoscope. Imaging was performed at 400 frames per second with an 8 μm spot size using a pullback to generate volumetric data over 16 mm with a pixel spacing of 5 μm in the longitudinal direction. Three-dimensional OCT (3D-OCT) imaging was performed in patients with a cross section of pathologies undergoing standard upper and lower endoscopy at the Veterans Affairs Boston Healthcare System (VABHS). Patients with Barrett's esophagus, dysplasia, and inflammatory bowel disease were imaged. The use of distally actuated imaging catheters allowed OCT imaging with more flexibility such as volumetric imaging in the terminal ileum and the assessment of the hiatal hernia using retroflex imaging. The high rotational stability of the micromotor enabled 3D volumetric imaging with micron scale volumetric accuracy for both en face and cross-sectional imaging. The ability to perform 3D OCT imaging in the GI tract with microscopic accuracy should enable a wide range of studies to investigate the ability of OCT to detect pathology as well as assess treatment response.

Handheld, rapidly switchable, anterior/posterior segment swept source optical coherence tomography probe

PubMed Central

Nankivil, Derek; Waterman, Gar; LaRocca, Francesco; Keller, Brenton; Kuo, Anthony N.; Izatt, Joseph A.

2015-01-01

We describe the first handheld, swept source optical coherence tomography (SSOCT) system capable of imaging both the anterior and posterior segments of the eye in rapid succession. A single 2D microelectromechanical systems (MEMS) scanner was utilized for both imaging modes, and the optical paths for each imaging mode were optimized for their respective application using a combination of commercial and custom optics. The system has a working distance of 26.1 mm and a measured axial resolution of 8 μm (in air). In posterior segment mode, the design has a lateral resolution of 9 μm, 7.4 mm imaging depth range (in air), 4.9 mm 6dB fall-off range (in air), and peak sensitivity of 103 dB over a 22° field of view (FOV). In anterior segment mode, the design has a lateral resolution of 24 μm, imaging depth range of 7.4 mm (in air), 6dB fall-off range of 4.5 mm (in air), depth-of-focus of 3.6 mm, and a peak sensitivity of 99 dB over a 17.5 mm FOV. In addition, the probe includes a wide-field iris imaging system to simplify alignment. A fold mirror assembly actuated by a bi-stable rotary solenoid was used to switch between anterior and posterior segment imaging modes, and a miniature motorized translation stage was used to adjust the objective lens position to correct for patient refraction between −12.6 and + 9.9 D. The entire probe weighs less than 630 g with a form factor of 20.3 x 9.5 x 8.8 cm. Healthy volunteers were imaged to illustrate imaging performance. PMID:26601014

Imaging collector channel entrance with a new intraocular micro-probe swept-source optical coherence tomography.

PubMed

Xin, Chen; Chen, Xiaoya; Li, Meng; Shi, Yan; Wang, Huaizhou; Wang, Ruikang; Wang, Ningli

2017-09-01

To describe the use of a newly developed side-viewing catheter probe to provide the cross-sectional images of collector channel entrance (CCE), achieved by swept-source optical coherence tomography (SS-OCT). A side-viewing SS-OCT catheter probe was developed that has a core probe diameter of 0.15 mm and an outer diameter of 0.25 mm, for the purpose of imaging CCEs within eye globe. Cadaver eyes harvested from swine and human were used to demonstrate its feasibility. For porcine eyes, the probe imaged the CCE by accessing the region of the aqueous plexus (AP) as well as along the inner wall (IW) of the trabecular meshwork (TM). For human eyes, the CCE images were captured by placing the probe within the lumen of the Schlemm's canal (SC) and along its IW. With the optical coherence tomography (OCT) catheter probe, the CCE is well delineated as optically empty areas within the highly scattering sclera. In porcine eyes, images captured in the region of the AP demonstrate a large cavity with delicate tissue strands around the probe. The CCE can be identified at the outer margin of the AP. When imaged along the IW, the TM is discernable but difficult to be distinguished from the AP. In the human limbal regions, when placed within the lumen of the SC, the catheter probe fully occupies the potential space. TM is highly compact. The CCE can be identified at the outer wall of the SC. When imaged along the IW of TM, the SC and CCE can be identified. The intraocular SS-OCT catheter probe is feasible to provide the CCE images, indicating useful clinical applications to assist glaucoma surgery. © 2017 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.

Decalcification using ethylenediaminetetraacetic acid for clear microstructure imaging of cochlea through optical coherence tomography

NASA Astrophysics Data System (ADS)

Lee, Jaeyul; Kim, Kanghae; Wijesinghe, Ruchire Eranga; Jeon, Doekmin; Lee, Sang Heun; Jeon, Mansik; Jang, Jeong Hun

2016-08-01

The aim of this study was to analyze the effectiveness of decalcification using ethylenediaminetetraacetic acid (EDTA) as an optical clearing method to enhance the depth visibility of internal soft tissues of cochlea. Ex vivo mouse and guinea pig cochlea samples were soaked in EDTA solutions for decalcification, and swept source optical coherence tomography (OCT) was used as imaging modality to monitor the decalcified samples consecutively. The monitored noninvasive cross-sectional images showed that the mouse and guinea pig cochlea samples had to be decalcified for subsequent 7 and 14 days, respectively, to obtain the optimal optical clearing results. Using this method, difficulties in imaging of internal cochlea microstructures of mice could be evaded. The obtained results verified that the depth visibility of the decalcified ex vivo samples was enhanced.

Microscope-integrated optical coherence tomography for image-aided positioning of glaucoma surgery

NASA Astrophysics Data System (ADS)

Li, Xiqi; Wei, Ling; Dong, Xuechuan; Huang, Ping; Zhang, Chun; He, Yi; Shi, Guohua; Zhang, Yudong

2015-07-01

Most glaucoma surgeries involve creating new aqueous outflow pathways with the use of a small surgical instrument. This article reported a microscope-integrated, real-time, high-speed, swept-source optical coherence tomography system (SS-OCT) with a 1310-nm light source for glaucoma surgery. A special mechanism was designed to produce an adjustable system suitable for use in surgery. A two-graphic processing unit architecture was used to speed up the data processing and real-time volumetric rendering. The position of the surgical instrument can be monitored and measured using the microscope and a grid-inserted image of the SS-OCT. Finally, experiments were simulated to assess the effectiveness of this integrated system. Experimental results show that this system is a suitable positioning tool for glaucoma surgery.

In vivo microvascular imaging of human oral and nasal cavities using swept-source optical coherence tomography with a single forward/side viewing probe

NASA Astrophysics Data System (ADS)

Choi, Woo June; Wang, Ruikang K.

2015-03-01

We report three-dimensional (3D) imaging of microcirculation within human cavity tissues in vivo using a high-speed swept-source optical coherence tomography (SS-OCT) at 1.3 μm with a modified probe interface. Volumetric structural OCT images of the inner tissues of oral and nasal cavities are acquired with a field of view of 2 mm x 2 mm. Two types of disposable and detachable probe attachments are devised and applied to the port of the imaging probe of OCT system, enabling forward and side imaging scans for selective and easy access to specific cavity tissue sites. Blood perfusion is mapped with OCT-based microangiography from 3D structural OCT images, in which a novel vessel extraction algorithm is used to decouple dynamic light scattering signals, due to moving blood cells, from the background scattering signals due to static tissue elements. Characteristic tissue anatomy and microvessel architectures of various cavity tissue regions of a healthy human volunteer are identified with the 3D OCT images and the corresponding 3D vascular perfusion maps at a level approaching capillary resolution. The initial finding suggests that the proposed method may be engineered into a promising tool for evaluating and monitoring tissue microcirculation and its alteration within a wide-range of cavity tissues in the patients with various pathological conditions.

All ceramic table tops analyzed using swept source optical coherence tomography

NASA Astrophysics Data System (ADS)

Stoica, Eniko Tunde; Marcauteanu, Corina; Sinescu, Cosmin; Negrutiu, Meda Lavinia; Topala, Florin; Duma, Virgil Florin; Bradu, Adrian; Podoleanu, Adrian Gh.

2016-03-01

Erosion is the progressive loss of tooth substance by chemical processes that do not involve bacterial action. The affected teeth can be restored by using IPS e.max Press "table tops", which replace the occlusal surfaces. In this study we applied a fast in-house Swept Source Optical Coherence Tomography (SS OCT) system to analyze IPS e.max Press "table tops". 12 maxillary first premolars have been extracted and prepared for "table tops". These restorations were subjected to 3000 alternating cycles of thermo-cycling in a range from -10°C to +50°C mechanical occlusal loads of 200 N were also applied. Using SS OCT we analyze the marginal seal of these restorations, before and after applying the mechanical and thermal strain. The characteristics of the SS OCT system utilized are presented. Its depth resolution, measured in air is 10 μm. The system is able to acquire entire volumetric reconstructions in 2.5 s. From the dataset acquired high resolution en-face projections were also produced. Thus, the interfaces between all ceramic "table tops" and natural teeth were analyzed on the cross-sections (i.e., the B-scans) produced and also on the volumetric (tri-dimensional (3D)) reconstructions, several open interfaces being detected. The study therefore demonstrates the utility of SS OCT for the analysis of lithium disilicate glass ceramic "table tops".

Pupil Tracking for Real-Time Motion Corrected Anterior Segment Optical Coherence Tomography

PubMed Central

Carrasco-Zevallos, Oscar M.; Nankivil, Derek; Viehland, Christian; Keller, Brenton; Izatt, Joseph A.

2016-01-01

Volumetric acquisition with anterior segment optical coherence tomography (ASOCT) is necessary to obtain accurate representations of the tissue structure and to account for asymmetries of the anterior eye anatomy. Additionally, recent interest in imaging of anterior segment vasculature and aqueous humor flow resulted in application of OCT angiography techniques to generate en face and 3D micro-vasculature maps of the anterior segment. Unfortunately, ASOCT structural and vasculature imaging systems do not capture volumes instantaneously and are subject to motion artifacts due to involuntary eye motion that may hinder their accuracy and repeatability. Several groups have demonstrated real-time tracking for motion-compensated in vivo OCT retinal imaging, but these techniques are not applicable in the anterior segment. In this work, we demonstrate a simple and low-cost pupil tracking system integrated into a custom swept-source OCT system for real-time motion-compensated anterior segment volumetric imaging. Pupil oculography hardware coaxial with the swept-source OCT system enabled fast detection and tracking of the pupil centroid. The pupil tracking ASOCT system with a field of view of 15 x 15 mm achieved diffraction-limited imaging over a lateral tracking range of +/- 2.5 mm and was able to correct eye motion at up to 22 Hz. Pupil tracking ASOCT offers a novel real-time motion compensation approach that may facilitate accurate and reproducible anterior segment imaging. PMID:27574800

In vivo layer visualization of rat olfactory bulb by a swept source optical coherence tomography and its confirmation through electrocoagulation and anatomy

PubMed Central

Watanabe, Hideyuki; Rajagopalan, Uma Maheswari; Nakamichi, Yu; Igarashi, Kei M.; Madjarova, Violeta Dimitrova; Kadono, Hirofumi; Tanifuji, Manabu

2011-01-01

Here, we report in vivo 3-D visualization of the layered organization of a rat olfactory bulb (OB) by a swept source optical coherence tomography (SS-OCT). The SS-OCT operates at a wavelength of 1334 nm with respective theoretical depth and lateral resolutions of 6.7 μm and 15.4 μm in air and hence it is possible to get a 3D structural map of OB in vivo at the micron level resolution with millimeter-scale imaging depth. Up until now, with methods such as MRI, confocal microscopy, OB depth structure in vivo had not been clearly visualized as these do not satisfy the criterion of simultaneously providing micron-scale spatial resolution and imaging up to a few millimeter in depth. In order to confirm the OB’s layered organization revealed by SS-OCT, we introduced the technique of electrocoagulation to make landmarks across the layered structure. To our knowledge this is such a first study that combines electrocoagulation and OCT in vivo of rat OB. Our results confirmed the layered organization of OB, and moreover the layers were clearly identified by electrocoagulation landmarks both in the OCT structural and anatomical slice images. We expect such a combined study is beneficial for both OCT and neuroscience fields. PMID:21833364

Ultra-high-speed optical coherence tomography with a stretched pulse supercontinuum source.

PubMed

Moon, Sucbei; Kim, Dug Young

2006-11-27

We introduce a new high-speed Fourier-domain optical coherence tomography (FD-OCT) scheme based on a stretched pulse supercontinuum source. A wide-band short pulse of a supercontinuum source of which output spectrum spanned a wavelength range from 1,200 nm to 1,550 nm was stretched to a long pulse of 70-ns duration by using a dispersive fiber due to the group-velocity dispersion, and it was used directly as frequency-swept light for FD-OCT. The OCT spectral interferogram was acquired in the time domain and converted into the spectral domain by the pre-calibrated time-to-wavelength relation. Using this stretched-pulse OCT (SP-OCT) scheme, we have demonstrated an ultrahigh-speed axial-line scanning rate of 5 MHz. The axial resolution of 8 microm was achieved without re-calibration of the sweep characteristic owing to the passive nature of the frequency-sweeping mechanism.

Evaluation of focal choroidal excavation in the macula using swept-source optical coherence tomography.

PubMed

Lim, F P M; Loh, B K; Cheung, C M G; Lim, L S; Chan, C M; Wong, D W K

2014-09-01

To evaluate imaging findings of patients with focal choroidal excavation (FCE) in the macula using swept-source optical coherence tomography (SS-OCT) and correlate it clinically. Prospective observational case series. Eleven consecutive patients (12 eyes) with FCE were described. Data on demographics and clinical presentation were collected and imaging findings (including color photography, fundus autofluorescence imaging, fluorescein angiography, indocyanine green angiography, spectral-domain optical coherence tomography, and SS-OCT) were analyzed. The primary diagnosis was epiretinal membrane (two eyes), choroidal neovascularization (one eye), polypoidal choroidal vasculopathy (three eyes), central serous chorioretinopathy (one eye), and dry age-related macular degeneration (two eyes). Eleven out of 12 of the lesions were conforming. One presented with a non-conforming lesion that progressed to a conforming lesion. One eye had multiFCE and two had two overlapping choroidal excavations. Using the SS-OCT, we found the choroid to be thinned out at the area of FCE but sclera remained normal. The choroidal tissue beneath the FCE was abnormal, with high internal reflectivity and poor visualization of choroidal vessels. There was loss of contour of the outer choroidal boundary that appeared to be pulled inward by this abnormal choroidal tissue. A suprachoroidal space was noted beneath this choroidal tissue and the choroidal-scleral interface was smooth. Repeat SS-OCT 6 months after presentation showed the area of excavation to be stable in size. FCE can be associated with epiretinal membrane, central serous chorioretinopathy, and age-related macular degeneration. The choroid was thinned out in the area of FCE.

Evaluation of focal choroidal excavation in the macula using swept-source optical coherence tomography

PubMed Central

Lim, F P M; Loh, B K; Cheung, C M G; Lim, L S; Chan, C M; Wong, D W K

2014-01-01

Purpose To evaluate imaging findings of patients with focal choroidal excavation (FCE) in the macula using swept-source optical coherence tomography (SS-OCT) and correlate it clinically. Methods Prospective observational case series. Eleven consecutive patients (12 eyes) with FCE were described. Data on demographics and clinical presentation were collected and imaging findings (including color photography, fundus autofluorescence imaging, fluorescein angiography, indocyanine green angiography, spectral-domain optical coherence tomography, and SS-OCT) were analyzed. Results The primary diagnosis was epiretinal membrane (two eyes), choroidal neovascularization (one eye), polypoidal choroidal vasculopathy (three eyes), central serous chorioretinopathy (one eye), and dry age-related macular degeneration (two eyes). Eleven out of 12 of the lesions were conforming. One presented with a non-conforming lesion that progressed to a conforming lesion. One eye had multiFCE and two had two overlapping choroidal excavations. Using the SS-OCT, we found the choroid to be thinned out at the area of FCE but sclera remained normal. The choroidal tissue beneath the FCE was abnormal, with high internal reflectivity and poor visualization of choroidal vessels. There was loss of contour of the outer choroidal boundary that appeared to be pulled inward by this abnormal choroidal tissue. A suprachoroidal space was noted beneath this choroidal tissue and the choroidal–scleral interface was smooth. Repeat SS-OCT 6 months after presentation showed the area of excavation to be stable in size. Conclusion FCE can be associated with epiretinal membrane, central serous chorioretinopathy, and age-related macular degeneration. The choroid was thinned out in the area of FCE. PMID:24946847

High throughput optical scanner

DOEpatents

Basiji, David A.; van den Engh, Gerrit J.

2001-01-01

A scanning apparatus is provided to obtain automated, rapid and sensitive scanning of substrate fluorescence, optical density or phosphorescence. The scanner uses a constant path length optical train, which enables the combination of a moving beam for high speed scanning with phase-sensitive detection for noise reduction, comprising a light source, a scanning mirror to receive light from the light source and sweep it across a steering mirror, a steering mirror to receive light from the scanning mirror and reflect it to the substrate, whereby it is swept across the substrate along a scan arc, and a photodetector to receive emitted or scattered light from the substrate, wherein the optical path length from the light source to the photodetector is substantially constant throughout the sweep across the substrate. The optical train can further include a waveguide or mirror to collect emitted or scattered light from the substrate and direct it to the photodetector. For phase-sensitive detection the light source is intensity modulated and the detector is connected to phase-sensitive detection electronics. A scanner using a substrate translator is also provided. For two dimensional imaging the substrate is translated in one dimension while the scanning mirror scans the beam in a second dimension. For a high throughput scanner, stacks of substrates are loaded onto a conveyor belt from a tray feeder.

Expanding Functionality of Commercial Optical Coherence Tomography Systems by Integrating a Custom Endoscope

PubMed Central

Welge, Weston A.; Barton, Jennifer K.

2015-01-01

Optical coherence tomography (OCT) is a useful imaging modality for detecting and monitoring diseases of the gastrointestinal tract and other tubular structures. The non-destructiveness of OCT enables time-serial studies in animal models. While turnkey commercial research OCT systems are plenty, researchers often require custom imaging probes. We describe the integration of a custom endoscope with a commercial swept-source OCT system and generalize this description to any imaging probe and OCT system. A numerical dispersion compensation method is also described. Example images demonstrate that OCT can visualize the mouse colon crypt structure and detect adenoma in vivo. PMID:26418811

Swept source optical coherence tomography for in vivo growth monitoring of capsicum annuum seeds treated with different NaCl concentrations

NASA Astrophysics Data System (ADS)

Ravichandran, Naresh Kumar; Wijesinghe, Ruchire Eranga; Lee, Seung-Yeol; Shirazi, Muhammad Faizan; Park, Kibeom; Jung, Hee-Young; Jeon, Mansik; Kim, Jeehyun

2017-04-01

In this study, Optical coherence tomography (OCT) is demonstrated as a plausible optical tool for in vivo detection of plant seeds and its morphological changes during growth. The experiment was carried out on Capsicum annuum seeds that were treated with different molar concentrations of NaCl to investigate the most optimal concentration for the seed growth. The monitoring process was carried out for 9 consecutive days. The in vivo 2D OCT images of the treated seeds were obtained and compared with seeds that were grown with sterile distilled water. The obtained results confirm the feasibility of using OCT for the proposed application. Normalized A-scan analysis method is utilized for supporting the concluded results.

Intravascular atherosclerotic imaging with combined fluorescence and optical coherence tomography probe based on a double-clad fiber combiner

NASA Astrophysics Data System (ADS)

Liang, Shanshan; Saidi, Arya; Jing, Joe; Liu, Gangjun; Li, Jiawen; Zhang, Jun; Sun, Changsen; Narula, Jagat; Chen, Zhongping

2012-07-01

We developed a multimodality fluorescence and optical coherence tomography probe based on a double-clad fiber (DCF) combiner. The probe is composed of a DCF combiner, grin lens, and micromotor in the distal end. An integrated swept-source optical coherence tomography and fluorescence intensity imaging system was developed based on the combined probe for the early diagnoses of atherosclerosis. This system is capable of real-time data acquisition and processing as well as image display. For fluorescence imaging, the inflammation of atherosclerosis and necrotic core formed with the annexin V-conjugated Cy5.5 were imaged. Ex vivo imaging of New Zealand white rabbit arteries demonstrated the capability of the combined system.

A Fiber Bragg Grating Sensor Interrogation System Based on a Linearly Wavelength-Swept Thermo-Optic Laser Chip

PubMed Central

Lee, Hyung-Seok; Lee, Hwi Don; Kim, Hyo Jin; Cho, Jae Du; Jeong, Myung Yung; Kim, Chang-Seok

2014-01-01

A linearized wavelength-swept thermo-optic laser chip was applied to demonstrate a fiber Bragg grating (FBG) sensor interrogation system. A broad tuning range of 11.8 nm was periodically obtained from the laser chip for a sweep rate of 16 Hz. To measure the linear time response of the reflection signal from the FBG sensor, a programmed driving signal was directly applied to the wavelength-swept laser chip. The linear wavelength response of the applied strain was clearly extracted with an R-squared value of 0.99994. To test the feasibility of the system for dynamic measurements, the dynamic strain was successfully interrogated with a repetition rate of 0.2 Hz by using this FBG sensor interrogation system. PMID:25177803

Picosecond pulses from wavelength-swept continuous-wave Fourier domain mode-locked lasers.

PubMed

Eigenwillig, Christoph M; Wieser, Wolfgang; Todor, Sebastian; Biedermann, Benjamin R; Klein, Thomas; Jirauschek, Christian; Huber, Robert

2013-01-01

Ultrafast lasers have a crucial function in many fields of science; however, up to now, high-energy pulses directly from compact, efficient and low-power semiconductor lasers are not available. Therefore, we introduce a new approach based on temporal compression of the continuous-wave, wavelength-swept output of Fourier domain mode-locked lasers, where a narrowband optical filter is tuned synchronously to the round-trip time of light in a kilometre-long laser cavity. So far, these rapidly swept lasers enabled orders-of-magnitude speed increase in optical coherence tomography. Here we report on the generation of ~60-70 ps pulses at 390 kHz repetition rate. As energy is stored optically in the long-fibre delay line and not as population inversion in the laser-gain medium, high-energy pulses can now be generated directly from a low-power, compact semiconductor-based oscillator. Our theory predicts subpicosecond pulses with this new technique in the future.

Picosecond pulses from wavelength-swept continuous-wave Fourier domain mode-locked lasers

NASA Astrophysics Data System (ADS)

Eigenwillig, Christoph M.; Wieser, Wolfgang; Todor, Sebastian; Biedermann, Benjamin R.; Klein, Thomas; Jirauschek, Christian; Huber, Robert

2013-05-01

Ultrafast lasers have a crucial function in many fields of science; however, up to now, high-energy pulses directly from compact, efficient and low-power semiconductor lasers are not available. Therefore, we introduce a new approach based on temporal compression of the continuous-wave, wavelength-swept output of Fourier domain mode-locked lasers, where a narrowband optical filter is tuned synchronously to the round-trip time of light in a kilometre-long laser cavity. So far, these rapidly swept lasers enabled orders-of-magnitude speed increase in optical coherence tomography. Here we report on the generation of ~60-70 ps pulses at 390 kHz repetition rate. As energy is stored optically in the long-fibre delay line and not as population inversion in the laser-gain medium, high-energy pulses can now be generated directly from a low-power, compact semiconductor-based oscillator. Our theory predicts subpicosecond pulses with this new technique in the future.

Asymmetry Analysis of Macular Inner Retinal Layers for Glaucoma Diagnosis: Swept-Source Optical Coherence Tomography Study.

PubMed

Lee, Sang-Yoon; Lee, Eun Kyoung; Park, Ki Ho; Kim, Dong Myung; Jeoung, Jin Wook

2016-01-01

To report an asymmetry analysis of macular inner retinal layers using swept-source optical coherence tomography (OCT) and to evaluate the utility for glaucoma diagnosis. Observational, cross-sectional study. Seventy normal healthy subjects and 62 glaucoma patients. Three-dimensional scans were acquired from 70 normal subjects and 62 open angle glaucoma patients by swept-source OCT. The thickness of the retinal nerve fiber layer, ganglion cell-inner plexiform layer (GCIPL), ganglion cell complex, and total retina were calculated within a 6.2×6.2 mm macular area divided into a 31×31 grid of 200×200 μm superpixels. For each of the corresponding superpixels, the thickness differences between the subject eyes and contra-lateral eyes and between the upper and lower macula halves of the subject eyes were determined. The negative differences were displayed on a gray-scale asymmetry map. Black superpixels were defined as thickness decreases over the cut-off values. The negative inter-ocular and inter-hemisphere differences in GCIPL thickness (mean ± standard deviation) were -2.78 ± 0.97 μm and -3.43 ± 0.71 μm in the normal group and -4.26 ± 2.23 μm and -4.88 ± 1.46 μm in the glaucoma group. The overall extent of the four layers' thickness decrease was larger in the glaucoma group than in the normal group (all Ps<0.05). The numbers of black superpixels on all of the asymmetry maps were larger in the glaucoma group than in the normal group (all Ps<0.05). The area under receiver operating characteristic curves of average negative thickness differences in macular inner layers for glaucoma diagnosis ranged from 0.748 to 0.894. The asymmetry analysis of macular inner retinal layers showed significant differences between the normal and glaucoma groups. The diagnostic performance of the asymmetry analysis was comparable to that of previous methods. These findings suggest that the asymmetry analysis can be a potential ancillary diagnostic tool.

Asymmetry Analysis of Macular Inner Retinal Layers for Glaucoma Diagnosis: Swept-Source Optical Coherence Tomography Study

PubMed Central

Lee, Sang-Yoon; Lee, Eun Kyoung; Park, Ki Ho; Kim, Dong Myung

2016-01-01

Purpose To report an asymmetry analysis of macular inner retinal layers using swept-source optical coherence tomography (OCT) and to evaluate the utility for glaucoma diagnosis. Design Observational, cross-sectional study. Participants Seventy normal healthy subjects and 62 glaucoma patients. Methods Three-dimensional scans were acquired from 70 normal subjects and 62 open angle glaucoma patients by swept-source OCT. The thickness of the retinal nerve fiber layer, ganglion cell-inner plexiform layer (GCIPL), ganglion cell complex, and total retina were calculated within a 6.2×6.2 mm macular area divided into a 31×31 grid of 200×200 μm superpixels. For each of the corresponding superpixels, the thickness differences between the subject eyes and contra-lateral eyes and between the upper and lower macula halves of the subject eyes were determined. The negative differences were displayed on a gray-scale asymmetry map. Black superpixels were defined as thickness decreases over the cut-off values. Results The negative inter-ocular and inter-hemisphere differences in GCIPL thickness (mean ± standard deviation) were -2.78 ± 0.97 μm and -3.43 ± 0.71 μm in the normal group and -4.26 ± 2.23 μm and -4.88 ± 1.46 μm in the glaucoma group. The overall extent of the four layers’ thickness decrease was larger in the glaucoma group than in the normal group (all Ps<0.05). The numbers of black superpixels on all of the asymmetry maps were larger in the glaucoma group than in the normal group (all Ps<0.05). The area under receiver operating characteristic curves of average negative thickness differences in macular inner layers for glaucoma diagnosis ranged from 0.748 to 0.894. Conclusions The asymmetry analysis of macular inner retinal layers showed significant differences between the normal and glaucoma groups. The diagnostic performance of the asymmetry analysis was comparable to that of previous methods. These findings suggest that the asymmetry analysis can be a potential ancillary diagnostic tool. PMID:27764166

Swept Source OCT Angiography of Neovascular Macular Telangiectasia Type 2

PubMed Central

Zhang, Qinqin; Wang, Ruikang K.; Chen, Chieh-Li; Legarreta, Andrew D.; Durbin, Mary K.; An, Lin; Sharma, Utkarsh; Stetson, Paul F.; Legarreta, John E.; Roisman, Luiz; Gregori, Giovanni; Rosenfeld, Philip J.

2015-01-01

Objective To image subretinal neovascularization in proliferative macular telangiectasia type 2 (MacTel2) using swept source optical coherence tomography based microangiography (OMAG). Study Design Patients with MacTel2 were enrolled in a prospective, observational study known as the MacTel Project and evaluated using a high-speed 1050nm swept-source OCT (SS-OCT) prototype system. The OMAG algorithm generated en face flow images from three retinal layers, as well as the region bounded by the outer retina and Bruch’s membrane, the choriocapillaris, and the remaining choroidal vasculature. The en face OMAG images were compared to images from fluorescein angiography (FA) and indocyanine green angiography (ICGA). Results Three eyes with neovascular MacTel2 were imaged. The neovascularization was best identified from the en face OMAG images that included a layer between the outer retinal boundary and Bruch’s membrane. OMAG images identified these abnormal vessels better than FA and were comparable to the images obtained using ICGA. In all three cases, OMAG identified choroidal vessels communicating with the neovascularization, and these choroidal vessels were evident in the two cases with ICGA imaging. In one case, monthly injections of bevacizumab reduced the microvascular complexity of the neovascularization, as well as the telangiectatic changes within the retinal microvasculature. In another case, less frequent bevacizumab therapy was associated with growth of the subretinal neovascular complex. Conclusions OMAG imaging provided detailed, depth-resolved information about subretinal neovascularization in MacTel2 eyes demonstrating superiority to FA imaging and similarities to ICGA imaging for documenting the retinal microvascular changes, the size and extent of the neovascular complex, the communications between the neovascular complex and the choroidal circulation, and the response to monthly bevacizumab therapy. PMID:26457402

Characterization of FBG sensor interrogation based on a FDML wavelength swept laser

PubMed Central

Jung, Eun Joo; Kim, Chang-Seok; Jeong, Myung Yung; Kim, Moon Ki; Jeon, Min Yong; Jung, Woonggyu; Chen, Zhongping

2012-01-01

In this study, we develop an ultra-fast fiber Bragg grating sensor system that is based on the Fourier domain mode-locked (FDML) swept laser. A FDML wavelength swept laser has many advantages compared to the conventional wavelength swept laser source, such as high-speed interrogation, narrow spectral sensitivity, and high phase stability. The newly developed FDML wavelength swept laser shows a superior performance of a high scan rate of 31.3 kHz and a broad scan range of over 70 nm simultaneously. The performance of the grating sensor interrogating system using a FDML wavelength swept laser is characterized in both static and dynamic strain responses. PMID:18852764

Polarization-sensitive optical coherence tomography using continuous polarization modulation with arbitrary phase modulation amplitude

NASA Astrophysics Data System (ADS)

Lu, Zenghai; Kasaragod, Deepa K.; Matcher, Stephen J.

2012-03-01

We demonstrate theoretically and experimentally that the phase retardance and relative optic-axis orientation of a sample can be calculated without prior knowledge of the actual value of the phase modulation amplitude when using a polarization-sensitive optical coherence tomography system based on continuous polarization modulation (CPM-PS-OCT). We also demonstrate that the sample Jones matrix can be calculated at any values of the phase modulation amplitude in a reasonable range depending on the system effective signal-to-noise ratio. This has fundamental importance for the development of clinical systems by simplifying the polarization modulator drive instrumentation and eliminating its calibration procedure. This was validated on measurements of a three-quarter waveplate and an equine tendon sample by a fiber-based swept-source CPM-PS-OCT system.

Local birefringence of the anterior segment of the human eye in a single capture with a full range polarisation-sensitive optical coherence tomography

NASA Astrophysics Data System (ADS)

Li, Qingyun; Karnowski, Karol; Villiger, Martin; Sampson, David D.

2017-04-01

A fibre-based full-range polarisation-sensitive optical coherence tomography system is developed to enable complete capture of the structural and birefringence properties of the anterior segment of the human eye in a single acquisition. The system uses a wavelength swept source centered at 1.3 μm, passively depth-encoded, orthogonal polarisation states in the illumination path and polarisation-diversity detection. Off-pivot galvanometer scanning is used to extend the imaging range and compensate for sensitivity drop-off. A Mueller matrix-based method is used to analyse data. We demonstrate the performance of the system and discuss issues relating to its optimisation.

Feasibility of speckle variance OCT for imaging cutaneous microvasculature regeneration during healing of wounds in diabetic mice

NASA Astrophysics Data System (ADS)

Sharma, P.; Kumawat, J.; Kumar, S.; Sahu, K.; Verma, Y.; Gupta, P. K.; Rao, K. D.

2018-02-01

We report on a study to assess the feasibility of a swept source-based speckle variance optical coherence tomography setup for monitoring cutaneous microvasculature. Punch wounds created in the ear pinnae of diabetic mice were monitored at different times post wounding to assess the structural and vascular changes. It was observed that the epithelium thickness increases post wounding and continues to be thick even after healing. Also, the wound size assessed by vascular images is larger than the physical wound size. The results show that the developed speckle variance optical coherence tomography system can be used to monitor vascular regeneration during wound healing in diabetic mice.

Elasticity measurement of nasal cartilage as a function of temperature using optical coherence elastography

NASA Astrophysics Data System (ADS)

Liu, Chih Hao; Skryabina, M. N.; Singh, Manmohan; Li, Jiasong; Wu, Chen; Sobol, E.; Larin, Kirill V.

2015-03-01

Current clinical methods of reconstruction surgery involve laser reshaping of nasal cartilage. The process of stress relaxation caused by laser heating is the primary method to achieve nasal cartilage reshaping. Based on this, a rapid, non-destructive and accurate elasticity measurement would allow for a more robust reshaping procedure. In this work, we have utilized a phase-stabilized swept source optical coherence elastography (PhSSSOCE) to quantify the Young's modulus of porcine nasal septal cartilage during the relaxation process induced by heating. The results show that PhS-SSOCE was able to monitor changes in elasticity of hyaline cartilage, and this method could potentially be applied in vivo during laser reshaping therapies.

Interferometric system based on swept source-optical coherence tomography scheme applied to the measurement of distances of industrial interest

NASA Astrophysics Data System (ADS)

Morel, Eneas N.; Russo, Nélida A.; Torga, Jorge R.; Duchowicz, Ricardo

2016-01-01

We used an interferometric technique based on typical optical coherence tomography (OCT) schemes for measuring distances of industrial interest. The system employed as a light source a tunable erbium-doped fiber laser of ˜20-pm bandwidth with a tuning range between 1520 and 1570 nm. It has a sufficiently long coherence length to enable long depth range imaging. A set of fiber Bragg gratings was used as a self-calibration method, which has the advantage of being a passive system that requires no additional electronic devices. The proposed configuration and the coherence length of the laser enlarge the range of maximum distances that can be measured with the common OCT configuration, maintaining a good axial resolution. A measuring range slightly >17 cm was determined. The system performance was evaluated by studying the repeatability and axial resolution of the results when the same optical path difference was measured. Additionally, the thickness of a semitransparent medium was also measured.

A pilot study to image the vascular network of small melanocytic choroidal tumors with speckle noise-free 1050-nm swept source optical coherence tomography (OCT choroidal angiography).

PubMed

Maloca, Peter; Gyger, Cyrill; Hasler, Pascal W

2016-06-01

To visualize and measure the vascular network of melanocytic choroidal tumors with speckle noise-free swept source optical coherence tomography (SS-OCT choroidal angiography). Melanocytic choroidal tumors from 24 eyes were imaged with 1050-nm optical coherence tomography (Topcon DRI OCT-1 Atlantis). A semi-automated algorithm was developed to remove speckle noise and to extract and measure the volume of the choroidal vessels from the obtained OCT data. In all cases, analysis of the choroidal vessels could be performed with SS-OCT without the need for pupillary dilation. The proposed method allows speckle noise-free, structure-guided visualization and measurement of the larger choroidal vessels in three dimensions. The obtained data suggest that speckle noise-free OCT may be more effective at identifying choroidal structures than traditional OCT methods. The measured volume of the extracted choroidal vessels of Haller's layer and Sattler's layer in the examined tumorous eyes was on average 0.982463955 mm(3) /982463956 μm(3) (range of 0.209764406 mm(3) /209764405.9 μm(3)to 1.78105544 mm(3) /1781055440 μm(3)). Full thickness obstruction of the choroidal vasculature by the tumor was found in 18 cases (72 %). In seven cases (18 %), choroidal vessel architecture did not show pronounced morphological abnormalities (18 %). Speckle noise-free OCT may serve as a new illustrative imaging technology and enhance visualization of the choroidal vessels without the need for dye injection. OCT can be used to identify and evaluate the choroidal vessels of melanocytic choroidal tumors, and may represent a potentially useful tool for imaging and monitoring of choroidal nevi and melanoma.

Efficiency and measurements agreement between swept-source OCT and low-coherence interferometry biometry systems.

PubMed

Calvo-Sanz, Jorge A; Portero-Benito, Alejandro; Arias-Puente, Alfonso

2018-03-01

To compare and evaluate the agreement between the measurements obtained with a swept-source optical coherence tomography (OCT)-based biometer, the IOLMaster® 700 (IOLM), and those obtained by an optical biometer based on optical low-coherence interferometry (OLCI), the Aladdin (ALD); To evaluate the ability to perform biometric measurements in those eyes with transparency alterations. Fifty-five eyes of 55 subjects were included in this study. Axial length (AL), corneal power (K, in diopters) and its astigmatism, anterior chamber depth (ACD), central corneal thickness (CCT), and lens thickness (LT) measures were obtained within both biometers, Zeiss IOLMaster 700 and Topcon Aladdin. Results were analyzed and compared using the Student's paired samples t-test, Bland-Altman analysis and intraclass correlation coefficient (ICC). Mean age was 73.12 ± 2.63 (62-89 years). The IOLM mean AL, K, and LT values did not show a statistically significant difference from ALD values and showed excellent agreement and correlation (ICC = 1.000, 0.970, 0.952). IOLM measured a lower mean ACD (-0.036 mm) and higher CCT measurements (9.296 μm). Those results were statistically different (p < 0.001 in both cases) but showed an excellent correlation coefficients (ICC = 0.994, 0.938). IOLM was able to obtain measures from all the eyes examined, while ALD did not measure in two cases with dense nuclear cataract. ALD showed spherical K measures in 7.27% of cases. Overall a quite good agreement between IOLM and ALD was found. ALD showed spherical keratometry measures in 7.27% of cases. IOLMaster 700 was more effective in obtaining AL measurements in eyes with dense cataracts.

Validation of Optical Coherence Tomography against Micro-computed Tomography for Evaluation of Remaining Coronal Dentin Thickness.

PubMed

Majkut, Patrycja; Sadr, Alireza; Shimada, Yasushi; Sumi, Yasunori; Tagami, Junji

2015-08-01

Optical coherence tomography (OCT) is a noninvasive modality to obtain in-depth images of biological structures. A dental OCT system has become available for chairside application. This in vitro study hypothesized that swept-source OCT can be used to measure the remaining dentin thickness (RDT) at the roof of the dental pulp chamber during excavation of deep caries. Human molar teeth with deep occlusal caries were investigated. After obtaining 2-dimensional and 3-dimensional OCT scans using a swept-source OCT system at a 1330-nm center wavelength, RDT was evaluated by image analysis software. Microfocus x-ray computed tomographic (micro-CT) images were obtained from the same cross sections to confirm OCT findings. The smallest RDT values at the visible pulp horn were measured on OCT and micro-CT imaging and compared using the Pearson correlation. Pulpal horns and pulp chamber roof observation under OCT and micro-CT imaging resulted in comparable images that allowed the measurement of coronal dentin thickness. RDT measured by OCT showed optical values range between 140 and 2300 μm, which corresponded to the range of 92-1524 μm on micro-CT imaging. A strong correlation was found between the 2 techniques (r = 0.96, P < .001). Further analysis indicated linear regression with a slope of 1.54 and no intercept, closely matching the bulk refractive index of dentin. OCT enables visualization of anatomic structures during deep caries excavation. Exposure of the vital dental pulp because of the removal of very thin remaining coronal dentin can be avoided with this novel noninvasive technique. Copyright © 2015 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Segmented swept source optical coherence tomography angiography assessment of the perifoveal vasculature in patients with X-linked juvenile retinoschisis: a serial case report.

PubMed

Stringa, Francesco; Tsamis, Emmanouli; Papayannis, Alessandro; Chwiejczak, Katarzyna; Jalil, Assad; Biswas, Susmito; Ahmad, Hassan; Stanga, Paulo Eduardo

2017-01-01

To describe perifoveal microvascular changes occurring in X-linked juvenile retinoschisis (XLRS) using swept source optical coherence tomography angiography (SS OCTA). This is a serial case report of three patients. Retrospective data of patients affected by XLRS were collected. Structural optical coherence tomography (OCT) and color fundus photography (CFPh) were carried out with Topcon ® OCT 2000 3D OCT as part of the standard care. Two patients were imaged on Topcon Atlantis ® SS OCTA and one on Topcon Triton ® SS OCTA. SS OCTA images were acquired using the 3 × 3 mm fovea-centered cubes scanning protocol. Analysis of both perifoveal superficial vascular plexus (pSVP) and perifoveal deep vascular plexus (pDVP) was performed by two observers after automated segmentation. Four eyes of three males (mean age 14 ± 3.8 years) were analyzed. All eyes showed foveoschisis on CFPh images. OCT B-scans of three eyes showed schistic cysts in the ganglion cell layer, inner nuclear layer (INL) and outer nuclear layer (ONL); in one eye, cysts were depicted in INL and ONL only. In two eyes, SS OCTA showed abnormal foveal avascular zone (FAZ) shape in the pSVP, and in the other two, FAZ shape was abnormal in both plexuses. In all eyes, retinal vascular abnormalities (ie, microvascular protrusions) were present in pDVP. SS OCTA can depict perifoveal microvascular changes in young patients affected by XLRS. In this study, the structural and vascular changes seem to be more evident in the pDVP and may represent a useful biomarker of prognosis.

Accuracy enhanced distance measurement system using double-sideband modulated frequency scanning interferometry

NASA Astrophysics Data System (ADS)

Chen, Xilun; Wang, Xiangchuan; Pan, Shilong

2017-03-01

An implementation of a distance measurement system using double-sideband with suppressed carrier modulation (DSB-SC) frequency scanning interferometry is proposed to reduce the variations in the optical path and improve the measurement accuracy. In this proposed system, the electro-optic DSB-SC is used to create dual-swept signals with opposite scanning directions. For each swept signal, the relative distance between the reference arm and the measuring arm is determined by the beat frequency of signals from two arms. By multiplying both beat signals, measurement errors caused by variations in the optical path can be greatly reduced. As an experimental demonstration, a vibration was introduced in the optical path length. The experimental results show that the variations can be suppressed for over 19.9 dB.

Longitudinal study of arteriogenesis with swept source optical coherence tomography and hyperspectral imaging

NASA Astrophysics Data System (ADS)

Poole, Kristin M.; Patil, Chetan A.; Nelson, Christopher E.; McCormack, Devin R.; Madonna, Megan C.; Duvall, Craig L.; Skala, Melissa C.

2014-03-01

Peripheral arterial disease (PAD) is an atherosclerotic disease of the extremities that leads to high rates of myocardial infarction and stroke, increased mortality, and reduced quality of life. PAD is especially prevalent in diabetic patients, and is commonly modeled by hind limb ischemia in mice to study collateral vessel development and test novel therapies. Current techniques used to assess recovery cannot obtain quantitative, physiological data non-invasively. Here, we have applied hyperspectral imaging and swept source optical coherence tomography (OCT) to study longitudinal changes in blood oxygenation and vascular morphology, respectively, intravitally in the diabetic mouse hind limb ischemia model. Additionally, recommended ranges for controlling physiological variability in blood oxygenation with respect to respiration rate and body core temperature were determined from a control animal experiment. In the longitudinal study with diabetic mice, hyperspectral imaging data revealed the dynamics of blood oxygenation recovery distally in the ischemic footpad. In diabetic mice, there is an early increase in oxygenation that is not sustained in the long term. Quantitative analysis of vascular morphology obtained from Hessian-filtered speckle variance OCT volumes revealed temporal dynamics in vascular density, total vessel length, and vessel diameter distribution in the adductor muscle of the ischemic limb. The combination of hyperspectral imaging and speckle variance OCT enabled acquisition of novel functional and morphological endpoints from individual animals, and provides a more robust platform for future preclinical evaluations of novel therapies for PAD.

High-speed swept source optical coherence Doppler tomography for deep brain microvascular imaging

NASA Astrophysics Data System (ADS)

Chen, Wei; You, Jiang; Gu, Xiaochun; Du, Congwu; Pan, Yingtian

2016-12-01

Noninvasive microvascular imaging using optical coherence Doppler tomography (ODT) has shown great promise in brain studies; however, high-speed microcirculatory imaging in deep brain remains an open quest. A high-speed 1.3 μm swept-source ODT (SS-ODT) system is reported which was based on a 200 kHz vertical-cavity-surface-emitting laser. Phase errors induced by sweep-trigger desynchronization were effectively reduced by spectral phase encoding and instantaneous correlation among the A-scans. Phantom studies have revealed a significant reduction in phase noise, thus an enhancement of minimally detectable flow down to 268.2 μm/s. Further in vivo validation was performed, in which 3D cerebral-blood-flow (CBF) networks in mouse brain over a large field-of-view (FOV: 8.5 × 5 × 3.2 mm3) was scanned through thinned skull. Results showed that fast flows up to 3 cm/s in pial vessels and minute flows down to 0.3 mm/s in arterioles or venules were readily detectable at depths down to 3.2 mm. Moreover, the dynamic changes of the CBF networks elicited by acute cocaine such as heterogeneous responses in various vessel compartments and at different cortical layers as well as transient ischemic events were tracked, suggesting the potential of SS-ODT for brain functional imaging that requires high flow sensitivity and dynamic range, fast frame rate and a large FOV to cover different brain regions.

Forward sweep, low noise rotor blade

NASA Technical Reports Server (NTRS)

Brooks, Thomas F. (Inventor)

1994-01-01

A forward-swept, low-noise rotor blade includes an inboard section, an aft-swept section, and a forward-swept outboard section. The rotor blade reduces the noise of rotorcraft, including both standard helicopters and advanced systems such as tiltrotors. The primary noise reduction feature is the forward sweep of the planform over a large portion of the outer blade radius. The rotor blade also includes an aft-swept section. The purpose of the aft-swept region is to provide a partial balance to pitching moments produced by the outboard forward-swept portion of the blade. The noise source showing maximum noise reduction is blade-vortex interaction (BVI) noise. Also reduced are thickness, noise, high speed impulsive noise, cabin vibration, and loading noise.

Axial Length Measurement Failure Rates With Biometers Using Swept-Source Optical Coherence Tomography Compared to Partial-Coherence Interferometry and Optical Low-Coherence Interferometry.

PubMed

McAlinden, Colm; Wang, Qinmei; Gao, Rongrong; Zhao, Weiqi; Yu, Ayong; Li, Yu; Guo, Yan; Huang, Jinhai

2017-01-01

To compare a new swept-source optical coherence tomography (SSOCT)-based biometer (OA-2000) with the IOLMaster v5.4 (partial-coherence interferometry) and Aladdin (optical low-coherence interferometry) biometers in terms of axial length measurement and failure rate in eyes with cataract. Reliability study. A total of 377 eyes of 210 patients were scanned with the 3 biometers in a random order. For each biometer, the number of unobtainable axial length measurements was recorded and grouped as per the type and severity of cataract based on the Lens Opacities Classification System III by the same experienced ophthalmologist. The Bland-Altman limits-of-agreement (LoA) method was used to assess the agreement in axial length measurements between the 3 biometers. The failure rate was 0 eyes (0%) with the OA-2000, 136 eyes (36.07%) with the IOLMaster, and 51 eyes (13.53%) with the Aladdin. χ 2 analyses indicated a significant difference in failure rate between all 3 devices (P < .001). Logistic regression analysis highlighted a statistically significant trend of higher failure rates with increasing severity of nuclear, cortical, and posterior subcapsular cataracts. Bland-Altman statistics indicated small mean differences and narrow LoA (OA-2000 vs IOLMaster -0.09 to 0.08 mm; OA-2000 vs Aladdin -0.10 to 0.07 mm; IOLMaster vs Aladdin -0.05 to 0.04 mm). The OA-2000, a new SSOCT-based biometer, outperformed both the IOLMaster and Aladdin biometers in very advanced cataracts of various morphologies. The use of SSOCT technology may be the reason for the improved performance of the OA-2000 and may lead to this technology becoming the gold standard for the measurement of axial length. Copyright © 2016 Elsevier Inc. All rights reserved.

Extended axial imaging range, widefield swept source optical coherence tomography angiography.

PubMed

Liu, Gangjun; Yang, Jianlong; Wang, Jie; Li, Yan; Zang, Pengxiao; Jia, Yali; Huang, David

2017-11-01

We developed a high-speed, swept source OCT system for widefield OCT angiography (OCTA) imaging. The system has an extended axial imaging range of 6.6 mm. An electrical lens is used for fast, automatic focusing. The recently developed split-spectrum amplitude and phase-gradient angiography allow high-resolution OCTA imaging with only two B-scan repetitions. An improved post-processing algorithm effectively removed trigger jitter artifacts and reduced noise in the flow signal. We demonstrated high contrast 3 mm×3 mm OCTA image with 400×400 pixels acquired in 3 seconds and high-definition 8 mm×6 mm and 12 mm×6 mm OCTA images with 850×400 pixels obtained in 4 seconds. A widefield 8 mm×11 mm OCTA image is produced by montaging two 8 mm×6 mm scans. An ultra-widefield (with a maximum of 22 mm along both vertical and horizontal directions) capillary-resolution OCTA image is obtained by montaging six 12 mm×6 mm scans. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Lens-based wavefront sensorless adaptive optics swept source OCT

NASA Astrophysics Data System (ADS)

Jian, Yifan; Lee, Sujin; Ju, Myeong Jin; Heisler, Morgan; Ding, Weiguang; Zawadzki, Robert J.; Bonora, Stefano; Sarunic, Marinko V.

2016-06-01

Optical coherence tomography (OCT) has revolutionized modern ophthalmology, providing depth resolved images of the retinal layers in a system that is suited to a clinical environment. Although the axial resolution of OCT system, which is a function of the light source bandwidth, is sufficient to resolve retinal features at a micrometer scale, the lateral resolution is dependent on the delivery optics and is limited by ocular aberrations. Through the combination of wavefront sensorless adaptive optics and the use of dual deformable transmissive optical elements, we present a compact lens-based OCT system at an imaging wavelength of 1060 nm for high resolution retinal imaging. We utilized a commercially available variable focal length lens to correct for a wide range of defocus commonly found in patient’s eyes, and a novel multi-actuator adaptive lens for aberration correction to achieve near diffraction limited imaging performance at the retina. With a parallel processing computational platform, high resolution cross-sectional and en face retinal image acquisition and display was performed in real time. In order to demonstrate the system functionality and clinical utility, we present images of the photoreceptor cone mosaic and other retinal layers acquired in vivo from research subjects.

Tunable, multiwavelength-swept fiber laser based on nematic liquid crystal device for fiber-optic electric-field sensor

NASA Astrophysics Data System (ADS)

Lee, Hyun Ji; Kim, Sung-Jo; Ko, Myeong Ock; Kim, Jong-Hyun; Jeon, Min Yong

2018-03-01

We propose a tunable multiwavelength-swept laser based on a nematic liquid crystal (NLC) Fabry-Perot (FP) etalon, which is embedded in the resonator of a wavelength-swept laser. We achieve the continuous wavelength tuning of the multiwavelength-swept laser by applying the electric field to the NLC FP etalon. The free spectral range of the fabricated NLC FP etalon is approximately 7.9 nm. When the electric field applied to the NLC FP etalon exceeds the threshold value (Fréedericksz threshold voltage), the output of the multiwavelength-swept laser can be tuned continuously. The tuning range of the multiwavelength-swept laser can be achieved at a value greater than 75 nm, which has a considerably wider tunable range than a conventional multiwavelength laser based on an NLC FP etalon. The slope efficiencies in the spectral and temporal domains for the tunable multiwavelength-swept laser are 22.2 nm/(mVrms / μm) and 0.17 ms/(mVrms / μm), respectively in the linear region. Therefore, the developed multiwavelength-swept laser based on the NLC FP etalon can be applied to an electric-field sensor. Because the wavelength measurement and time measurement have a linear relationship, the electric-field sensor can detect a rapid change in the electric-field intensity by measuring the peak change of the pulse in the temporal domain using the NLC FP etalon-based multiwavelength-swept laser.

Polarization-sensitive optical coherence tomography measurements with different phase modulation amplitude when using continuous polarization modulation

NASA Astrophysics Data System (ADS)

Lu, Zenghai; Kasaragod, Deepa K.; Matcher, Stephen J.

2012-01-01

We demonstrate theoretically and experimentally that the phase retardance and relative optic-axis orientation of a sample can be calculated without prior knowledge of the actual value of the phase modulation amplitude when using a polarization-sensitive optical coherence tomography system based on continuous polarization modulation (CPM-PS-OCT). We also demonstrate that the sample Jones matrix can be calculated at any values of the phase modulation amplitude in a reasonable range depending on the system effective signal-to-noise ratio. This has fundamental importance for the development of clinical systems by simplifying the polarization modulator drive instrumentation and eliminating its calibration procedure. This was validated on measurements of a three-quarter waveplate and an equine tendon sample by a fiber-based swept-source CPM-PS-OCT system.

Precision and broadband frequency swept laser source based on high-order modulation-sideband injection-locking.

PubMed

Wei, Fang; Lu, Bin; Wang, Jian; Xu, Dan; Pan, Zhengqing; Chen, Dijun; Cai, Haiwen; Qu, Ronghui

2015-02-23

A precision and broadband laser frequency swept technique is experimentally demonstrated. Using synchronous current compensation, a slave diode laser is dynamically injection-locked to a specific high-order modulation-sideband of a narrow-linewidth master laser modulated by an electro-optic modulator (EOM), whose driven radio frequency (RF) signal can be agilely, precisely controlled by a frequency synthesizer, and the high-order modulation-sideband enables multiplied sweep range and tuning rate. By using 5th order sideband injection-locking, the original tuning range of 3 GHz and tuning rate of 0.5 THz/s is multiplied by 5 times to 15 GHz and 2.5 THz/s respectively. The slave laser has a 3 dB-linewidth of 2.5 kHz which is the same to the master laser. The settling time response of a 10 MHz frequency switching is 2.5 µs. By using higher-order modulation-sideband and optimized experiment parameters, an extended sweep range and rate could be expected.

Real-time polarization-sensitive optical coherence tomography data processing with parallel computing

PubMed Central

Liu, Gangjun; Zhang, Jun; Yu, Lingfeng; Xie, Tuqiang; Chen, Zhongping

2010-01-01

With the increase of the A-line speed of optical coherence tomography (OCT) systems, real-time processing of acquired data has become a bottleneck. The shared-memory parallel computing technique is used to process OCT data in real time. The real-time processing power of a quad-core personal computer (PC) is analyzed. It is shown that the quad-core PC could provide real-time OCT data processing ability of more than 80K A-lines per second. A real-time, fiber-based, swept source polarization-sensitive OCT system with 20K A-line speed is demonstrated with this technique. The real-time 2D and 3D polarization-sensitive imaging of chicken muscle and pig tendon is also demonstrated. PMID:19904337

Ophthalmic laser system integrated with speckle variance optical coherence tomography for real-time temperature monitoring

NASA Astrophysics Data System (ADS)

Lee, Soohyun; Lee, Changho; Cheon, Gyeongwoo; Kim, Jongmin; Jo, Dongki; Lee, Jihoon; Kang, Jin U.

2018-02-01

A commercial ophthalmic laser system (R;GEN, Lutronic Corp) was integrated with a swept-source optical coherence tomography (OCT) imaging system for real-time tissue temperature monitoring. M-scan OCT images were acquired during laser-pulse radiation, and speckle variance OCT (svOCT) images were analyzed to deduce temporal signal variations related to tissue temperature change from laser-pulse radiation. A phantom study shows that svOCT magnitude increases abruptly after laser pulse radiation and recovered exponentially, and the peak intensity of svOCT image was linearly dependent on pulse laser energy until it saturates. A study using bovine iris also showed signal variation dependence on the laser pulse radiation, and the variation was more distinctive with higher energy level.

On-chip Mach-Zehnder interferometer for OCT systems

NASA Astrophysics Data System (ADS)

van Leeuwen, Ton G.; Akca, Imran B.; Angelou, Nikolaos; Weiss, Nicolas; Hoekman, Marcel; Leinse, Arne; Heideman, Rene G.

2018-04-01

By using integrated optics, it is possible to reduce the size and cost of a bulky optical coherence tomography (OCT) system. One of the OCT components that can be implemented on-chip is the interferometer. In this work, we present the design and characterization of a Mach-Zehnder interferometer consisting of the wavelength-independent splitters and an on-chip reference arm. The Si3N4 was chosen as the material platform as it can provide low losses while keeping the device size small. The device was characterized by using a home-built swept source OCT system. A sensitivity value of 83 dB, an axial resolution of 15.2 μm (in air) and a depth range of 2.5 mm (in air) were all obtained.

Speckle size in optical Fourier domain imaging

NASA Astrophysics Data System (ADS)

Lamouche, G.; Vergnole, S.; Bisaillon, C.-E.; Dufour, M.; Maciejko, R.; Monchalin, J.-P.

2007-06-01

As in conventional time-domain optical coherence tomography (OCT), speckle is inherent to any Optical Fourier Domain Imaging (OFDI) of biological tissue. OFDI is also known as swept-source OCT (SS-OCT). The axial speckle size is mainly determined by the OCT resolution length and the transverse speckle size by the focusing optics illuminating the sample. There is also a contribution from the sample related to the number of scatterers contained within the probed volume. In the OFDI data processing, there is some liberty in selecting the range of wavelengths used and this allows variation in the OCT resolution length. Consequently the probed volume can be varied. By performing measurements on an optical phantom with a controlled density of discrete scatterers and by changing the probed volume with different range of wavelengths in the OFDI data processing, there is an obvious change in the axial speckle size, but we show that there is also a less obvious variation in the transverse speckle size. This work contributes to a better understanding of speckle in OCT.

Megahertz ultra-wide-field swept-source retina optical coherence tomography compared to current existing imaging devices.

PubMed

Reznicek, Lukas; Klein, Thomas; Wieser, Wolfgang; Kernt, Marcus; Wolf, Armin; Haritoglou, Christos; Kampik, Anselm; Huber, Robert; Neubauer, Aljoscha S

2014-06-01

To investigate the image quality of wide-angle cross-sectional and reconstructed fundus images based on ultra-megahertz swept-source Fourier domain mode locking (FDML) OCT compared to current generation diagnostic devices. A 1,050 nm swept-source FDML OCT system was constructed running at 1.68 MHz A-scan rate covering approximately 70° field of view. Twelve normal eyes were imaged with the device applying an isotropically dense sampling protocol (1,900 × 1,900 A-scans) with a fill factor of 100 %. Obtained OCT scan image quality was compared with two commercial OCT systems (Heidelberg Spectralis and Stratus OCT) of the same 12 eyes. Reconstructed en-face fundus images from the same FDML-OCT data set were compared to color fundus, infrared and ultra-wide-field scanning laser images (SLO). Comparison of cross-sectional scans showed a high overall image quality of the 15× averaged FDML images at 1.68 MHz [overall quality grading score: 8.42 ± 0.52, range 0 (bad)-10 (excellent)] comparable to current spectral-domain OCTs (overall quality grading score: 8.83 ± 0.39, p = 0.731). On FDML OCT, a dense 3D data set was obtained covering also the central and mid-peripheral retina. The reconstructed FDML OCT en-face fundus images had high image quality comparable to scanning laser ophthalmoscope (SLO) as judged from retinal structures such as vessels and optic disc. Overall grading score was 8.36 ± 0.51 for FDML OCT vs 8.27 ± 0.65 for SLO (p = 0.717). Ultra-wide-field megahertz 3D FDML OCT at 1.68 MHz is feasible, and provides cross-sectional image quality comparable to current spectral-domain OCT devices. In addition, reconstructed en-face visualization of fundus images result in a wide-field view with high image quality as compared to currently available fundus imaging devices. The improvement of >30× in imaging speed over commercial spectral-domain OCT technology enables high-density scan protocols leading to a data set for high quality cross-sectional and en-face images of the posterior segment.

Multi-volumetric registration and mosaicking using swept-source spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography

NASA Astrophysics Data System (ADS)

Bozic, Ivan; El-Haddad, Mohamed T.; Malone, Joseph D.; Joos, Karen M.; Patel, Shriji N.; Tao, Yuankai K.

2017-02-01

Ophthalmic diagnostic imaging using optical coherence tomography (OCT) is limited by bulk eye motions and a fundamental trade-off between field-of-view (FOV) and sampling density. Here, we introduced a novel multi-volumetric registration and mosaicking method using our previously described multimodal swept-source spectrally encoded scanning laser ophthalmoscopy and OCT (SS-SESLO-OCT) system. Our SS-SESLO-OCT acquires an entire en face fundus SESLO image simultaneously with every OCT cross-section at 200 frames-per-second. In vivo human retinal imaging was performed in a healthy volunteer, and three volumetric datasets were acquired with the volunteer moving freely and refixating between each acquisition. In post-processing, SESLO frames were used to estimate en face rotational and translational motions by registering every frame in all three volumetric datasets to the first frame in the first volume. OCT cross-sections were contrast-normalized and registered axially and rotationally across all volumes. Rotational and translational motions calculated from SESLO frames were applied to corresponding OCT B-scans to compensate for interand intra-B-scan bulk motions, and the three registered volumes were combined into a single interpolated multi-volumetric mosaic. Using complementary information from SESLO and OCT over serially acquired volumes, we demonstrated multivolumetric registration and mosaicking to recover regions of missing data resulting from blinks, saccades, and ocular drifts. We believe our registration method can be directly applied for multi-volumetric motion compensation, averaging, widefield mosaicking, and vascular mapping with potential applications in ophthalmic clinical diagnostics, handheld imaging, and intraoperative guidance.

Can Probability Maps of Swept-Source Optical Coherence Tomography Predict Visual Field Changes in Preperimetric Glaucoma?

PubMed

Lee, Won June; Kim, Young Kook; Jeoung, Jin Wook; Park, Ki Ho

2017-12-01

To determine the usefulness of swept-source optical coherence tomography (SS-OCT) probability maps in detecting locations with significant reduction in visual field (VF) sensitivity or predicting future VF changes, in patients with classically defined preperimetric glaucoma (PPG). Of 43 PPG patients, 43 eyes were followed-up on every 6 months for at least 2 years were analyzed in this longitudinal study. The patients underwent wide-field SS-OCT scanning and standard automated perimetry (SAP) at the time of enrollment. With this wide-scan protocol, probability maps originating from the corresponding thickness map and overlapped with SAP VF test points could be generated. We evaluated the vulnerable VF points with SS-OCT probability maps as well as the prevalence of locations with significant VF reduction or subsequent VF changes observed in the corresponding damaged areas of the probability maps. The vulnerable VF points were shown in superior and inferior arcuate patterns near the central fixation. In 19 of 43 PPG eyes (44.2%), significant reduction in baseline VF was detected within the areas of structural change on the SS-OCT probability maps. In 16 of 43 PPG eyes (37.2%), subsequent VF changes within the areas of SS-OCT probability map change were observed over the course of the follow-up. Structural changes on SS-OCT probability maps could detect or predict VF changes using SAP, in a considerable number of PPG eyes. Careful comparison of probability maps with SAP results could be useful in diagnosing and monitoring PPG patients in the clinical setting.

Apices of maxillary premolars observed by swept source optical coherence tomography

NASA Astrophysics Data System (ADS)

Ebihara, Arata; Iino, Yoshiko; Yoshioka, Toshihiko; Hanada, Takahiro; Sunakawa, Mitsuhiro; Sumi, Yasunori; Suda, Hideaki

2015-02-01

Apicoectomy is performed for the management of apical periodontitis when orthograde root canal treatment is not possible or is ineffective. Prior to the surgery, cone beam computed tomography (CBCT) examination is often performed to evaluate the lesion and the adjacent tissues. During the surgical procedure, the root apex is resected and the resected surface is usually observed under dental operating microscope (DOM). However, it is difficult to evaluate the details and the subsurface structure of the root using CBCT and DOM. A new diagnostic system, swept source optical coherence tomography (SS-OCT), has been developed to observe the subsurface anatomical structure. The aim of this study was to observe resected apical root canals of human maxillary premolars using SS-OCT and compare the findings with those observed using CBCT and DOM. Six extracted human maxillary premolars were used. After microfocus computed tomography (Micro CT; for gold standard) and CBCT scanning of the root, 1 mm of the apex was cut perpendicular to the long axis of the tooth. Each resected surface was treated with EDTA, irrigated with saline solution, and stained with methylene blue dye. The resected surface was observed with DOM and SS-OCT. This sequence was repeated three times. The number of root canals was counted and statistically evaluated. There was no significant difference in the accuracy of detecting root canals among CBCT, DOM and SS-OCT (p > 0.05, Wilcoxon test). Because SS-OCT can be used in real time during surgery, it would be a useful tool for observing resected apical root canals.

Investigation of firing temperature variation in ovens for ceramic-fused-to-metal dental prostheses using swept source optical coherence tomography

NASA Astrophysics Data System (ADS)

Todor, Raluca; Negrutiu, Meda-Lavinia; Sinescu, Cosmin; Topala, Florin Ionel; Bradu, Adrian; Duma, Virgil-Florin; Romînu, Mihai; Podoleanu, Adrian G.

2018-03-01

One of the most common fabrication techniques for dental ceramics is sintering, a process of heating of the ceramic to ensure densification. This occurs by viscous flow when the firing temperature is reached. Acceptable restorations require the alloy and ceramic to be chemically, thermally, mechanically, and aesthetically compatible. Thermal and mechanical compatibility include a fusing temperature of ceramic that does not cause distortion of the metal substructure. Decalibration of ovens used for firing of the ceramic layers for metal ceramic dental prostheses leads to stress and cracks in the veneering material, and ultimately to the failure of the restoration. 25 metal ceramic prostheses were made for this study. They were divided in five groups, each sintered at a different temperature: a group at the temperature prescribed by the producer, two groups at lower and two groups at higher temperatures set in the ceramic oven. An established noninvasive biomedical imaging method, swept source (SS) optical coherence tomography (OCT) was employed, in order to evaluate the modifications induced when using temperatures different from those prescribed for firing the samples. A quantitative assessment of the probes is performed by en-face OCT images, taken at constant depths inside the samples. The differences in granulation, thus in reflectivity allow for extracting rules-of-thumb to evaluate fast, by using only the prostheses currently produced the current calibration of the ceramic oven. OCT imaging can allow quick identification of the oven decalibration, to avoid producing dental prostheses with defects.

Three-Dimensional Cataract Crystalline Lens Imaging With Swept-Source Optical Coherence Tomography.

PubMed

de Castro, Alberto; Benito, Antonio; Manzanera, Silvestre; Mompeán, Juan; Cañizares, Belén; Martínez, David; Marín, Jose María; Grulkowski, Ireneusz; Artal, Pablo

2018-02-01

To image, describe, and characterize different features visible in the crystalline lens of older adults with and without cataract when imaged three-dimensionally with a swept-source optical coherence tomography (SS-OCT) system. We used a new SS-OCT laboratory prototype designed to enhance the visualization of the crystalline lens and imaged the entire anterior segment of both eyes in two groups of participants: patients scheduled to undergo cataract surgery, n = 17, age range 36 to 91 years old, and volunteers without visual complains, n = 14, age range 20 to 81 years old. Pre-cataract surgery patients were also clinically graded according to the Lens Opacification Classification System III. The three-dimensional location and shape of the visible opacities were compared with the clinical grading. Hypo- and hyperreflective features were visible in the lens of all pre-cataract surgery patients and in some of the older adults in the volunteer group. When the clinical examination revealed cortical or subcapsular cataracts, hyperreflective features were visible either in the cortex parallel to the surfaces of the lens or in the posterior pole. Other type of opacities that appeared as hyporeflective localized features were identified in the cortex of the lens. The OCT signal in the nucleus of the crystalline lens correlated with the nuclear cataract clinical grade. A dedicated OCT is a useful tool to study in vivo the subtle opacities in the cataractous crystalline lens, revealing its position and size three-dimensionally. The use of these images allows obtaining more detailed information on the age-related changes leading to cataract.

Classification of Exudative Age-Related Macular Degeneration With Pachyvessels on En Face Swept-Source Optical Coherence Tomography.

PubMed

Ng, Danny Siu-Chun; Bakthavatsalam, Malini; Lai, Frank Hiu-Ping; Cheung, Carol Yim-Lui; Cheung, Gemmy Chu-Ming; Tang, Fang Yao; Tsang, Chi Wai; Lai, Timothy Yuk-Yau; Wong, Tien Yin; Brelén, Mårten Erik

2017-02-01

The purpose of this study was to classify exudative maculopathy by the presence of pachyvessels on en face swept-source optical coherence tomography (SSOCT). Consecutive patients with signs of exudative maculopathy underwent SSOCT, fluorescein and indocyanine green angiography (ICGA), ultra-widefield fundus color photography, and autofluorescence examinations. Images were analyzed in a masked fashion by two sets of four examiners in different sessions: (1) the presence of pachyvessels in en face OCT and (2) features of exudative maculopathy in conventional imaging modalities. Quantitative data obtained were subfoveal choroidal thickness (SFCT) and choroidal vascularity index (CVI), which was the ratio of choroidal vessels lumen area to a specified choroidal area from binarized cross-sectional OCT scans. Pachyvessels was observed in 38 (52.1%) of 73 eyes. The pachyvessels group was associated with younger age (69.1 ± 9.4 years, odds ratio [OR] = 0.95, 95% confidence interval [95% CI] = 0.90-0.97, P = 0.04), presence of polypoidal lesions (OR = 3.27, 95% CI = 1.24-8.62, P = 0.01), increased SFCT (OR = 1.08, 95% CI = 1.02-1.14, P < 0.01), and increased CVI (65.4 ± 5.3, OR = 1.12, 95% CI = 1.02-1.23, P = 0.01). In multivariate regression, CVI significantly correlated with pachyvessels (OR = 1.24, 95% CI = 1.03-1.55, P = 0.04). Exudative maculopathy could be classified based on differences in choroidal vasculature morphology. Current results implied that choroidal hemodynamics may be relevant to variable natural history and treatment response in neovascular AMD and polypoidal choroidal vasculopathy.

Observation of the pulp horn by swept source optical coherence tomography and cone beam computed tomography

NASA Astrophysics Data System (ADS)

Iino, Yoshiko; Yoshioka, Toshihiko; Hanada, Takahiro; Ebihara, Arata; Sunakawa, Mitsuhiro; Sumi, Yasunori; Suda, Hideaki

2015-02-01

Cone-beam computed tomography (CBCT) is one of the most useful diagnostic techniques in dentistry but it involves ionizing radiation, while swept source optical coherence tomography (SS-OCT) has been introduced recently as a nondestructive, real-time, high resolution imaging technique using low-coherence interferometry, which involves no ionizing radiation. The purpose of this study was to evaluate the ability of SS-OCT to detect the pulp horn (PH) in comparison with that of CBCT. Ten extracted human mandibular molars were used. After horizontally removing a half of the tooth crown, the distance from the cut dentin surface to PH was measured using microfocus computed tomography (Micro CT) (SL) as the gold standard, by CBCT (CL) and by SS-OCT (OL). In the SS-OCT images, only when PH was observed beneath the overlying dentin, the distance from the cut dentin surface to PH was recorded. If the pulp was exposed, it was defined as pulp exposure (PE). The results obtained by the above three methods were statistically analyzed by Spearman's rank correlation coefficient at a significance level of p < 0.01. SS-OCT detected the presence of PH when the distance from the cut dentin surface to PH determined by SL was 2.33 mm or less. Strong correlations of the measured values were found between SL and CL (r=0.87), SL and OL (r=0.96), and CL and OL (r=0.86). The results showed that SS-OCT images correlated closely with CBCT images, suggesting that SS-OCT can be a useful tool for the detection of PH.

Imaging whole mouse brains with a dual resolution serial swept-source optical coherence tomography scanner

NASA Astrophysics Data System (ADS)

Lefebvre, Joël.; Castonguay, Alexandre; Lesage, Frédéric

2018-02-01

High resolution imaging of whole rodent brains using serial OCT scanners is a promising method to investigate microstructural changes in tissue related to the evolution of neuropathologies. Although micron to sub-micron sampling resolution can be obtained by using high numerical aperture objectives and dynamic focusing, such an imaging system is not adapted to whole brain imaging. This is due to the large amount of data it generates and the significant computational resources required for reconstructing such volumes. To address this limitation, a dual resolution serial OCT scanner was developed. The optical setup consists in a swept-source OCT made of two sample and reference arms, each arm being coupled with different microscope objectives (3X / 40X). Motorized flip mirrors were used to switch between each OCT arm, thus allowing low and high resolution acquisitions within the same sample. The low resolution OCT volumes acquired with the 3X arm were stitched together, providing a 3D map of the whole mouse brain. This brain can be registered to an OCT brain template to enable neurological structures localization. The high resolution volumes acquired with the 40X arm were also stitched together to create local high resolution 3D maps of the tissue microstructure. The 40X data can be acquired at any arbitrary location in the sample, thus limiting storage-heavy high resolution data to application restricted to specific regions of interest. By providing dual-resolution OCT data, this setup can be used to validate diffusion MRI with tissue microstructure derived metrics measured at any location in ex vivo brains.

Factors Affecting Choroidal Vascular Density in Normal Eyes: Quantification Using En Face Swept-Source Optical Coherence Tomography.

PubMed

Fujiwara, Atsushi; Morizane, Yuki; Hosokawa, Mio; Kimura, Shuhei; Kumase, Fumiaki; Shiode, Yusuke; Doi, Shinichiro; Hirano, Masayuki; Toshima, Shinji; Hosogi, Mika; Shiraga, Fumio

2016-10-01

To quantify the vascular density of the choroid of normal eyes and to identify the influencing factors using en face images obtained with swept-source optical coherence tomography (SS OCT). Prospective cross-sectional study. One hundred and sixty-three eyes of 163 healthy volunteers (83 female; mean age 42.2 ± 22.6 years) with a corrected visual acuity of ≥1.0 were investigated. En face SS OCT images of the choroid were used for quantitative assessment of the vascular density in the large choroid vessel layer. Relationships between vascular density of the choroid and age, sex, refractive error (RE), axial length (AL), and subfoveal choroidal thickness (SCT) were also investigated. There was a significant negative relationship between vascular density of the choroid and subject age (P < .001). Analysis according to age showed a significant correlation in the group aged >30 years (P < .001), but not in the group aged ≤30 years (P = .225). SCT had a significant positive relationship with vascular density of the choroid (P < .001). However, a significant correlation was not observed between sex, RE, or AL and vascular density of the choroid (P = .981, P = .292, and P = .216, respectively). Multivariable regression analysis with vascular density of the choroid as the dependent variable and age, sex, RE, AL, and SCT as independent variables showed that age and SCT are important determinants of vascular density of the choroid (P < .001). Age and SCT affect vascular density of the choroid. Copyright © 2016 Elsevier Inc. All rights reserved.

Laser a balayage spectral double-bande pour l'imagerie biomedicale multimodale

NASA Astrophysics Data System (ADS)

Goulamhoussen, Nadir

A novel swept laser providing simultaneous dual-band (780nm and 1 300 nm) wavelength scanning has been designed for use in multimodal imaging systems. The swept laser is based on two gain media : a fibered semiconductor optical amplifier (SOA) centered at 1 300nm and a free-space laser diode centered at 780 nm. Simultaneous wavelength tuning for both bands is obtained by separate wavelength filters set up around the same rotating polygonal mirror. For each band, a telescope in an infinite conjugate setup converges the wavelengths dispersed by a grating on the polygon. The polygon reflects back a narrow band of wavelengths for amplification in the gain medium. Rotating the polygon enables wavelength tuning and imaging at a rate of 6 000 to 30 000 spectral lines/s, or A-lines/s in Optical Coherence Tomography (OCT). The 780nm source has a bandwidth of 37 nm, a fibered output power of 54 mW and a coherence length of 11 mm. The 1 300nm source has a bandwidth of 75 nm, a fibered output power of 17mW and a coherence length of 7.2 mm. Three multimodal systems were designed to test the potential of the swept laser in biomedical imaging. A two color OCT which allows three-dimensional in depth imaging of biological tissues with good morphological contrast was first designed, including a novel arrangement for balanced detection in both bands. A simultaneous OCT and SECM instrument was also built in which spectrally encoded confocal microscopy (SECM) provides en face images of subcellular features with high resolution on top of the 3D high penetration image obtained by OCT. Finally, a system combining OCT with fluorescence was designed, thus adding functional imaging to structural OCT images. There are many prospective paths for these three modalities, first among them the adaptation of the systems such that they may be used with imaging probes. One potential solution would be the development of novel fiber components to combine the illumination of theses modalities while demultiplexing their detection, and as would be the development of new optomechanics to enable 3D real-time in vivo imaging.

In vivo imaging of the rodent eye with swept source/Fourier domain OCT

PubMed Central

Liu, Jonathan J.; Grulkowski, Ireneusz; Kraus, Martin F.; Potsaid, Benjamin; Lu, Chen D.; Baumann, Bernhard; Duker, Jay S.; Hornegger, Joachim; Fujimoto, James G.

2013-01-01

Swept source/Fourier domain OCT is demonstrated for in vivo imaging of the rodent eye. Using commercial swept laser technology, we developed a prototype OCT imaging system for small animal ocular imaging operating in the 1050 nm wavelength range at an axial scan rate of 100 kHz with ~6 µm axial resolution. The high imaging speed enables volumetric imaging with high axial scan densities, measuring high flow velocities in vessels, and repeated volumetric imaging over time. The 1050 nm wavelength light provides increased penetration into tissue compared to standard commercial OCT systems at 850 nm. The long imaging range enables multiple operating modes for imaging the retina, posterior eye, as well as anterior eye and full eye length. A registration algorithm using orthogonally scanned OCT volumetric data sets which can correct motion on a per A-scan basis is applied to compensate motion and merge motion corrected volumetric data for enhanced OCT image quality. Ultrahigh speed swept source OCT is a promising technique for imaging the rodent eye, proving comprehensive information on the cornea, anterior segment, lens, vitreous, posterior segment, retina and choroid. PMID:23412778

A Comparison of Potential IM-CW Lidar Modulation Techniques for ASCENDS CO2 Column Measurements From Space

NASA Technical Reports Server (NTRS)

Campbell, Joel F.; Lin, Bing; Nehrir, Amin R.; Harrison, F. Wallace; Obland, Michael D.; Ismail, Syed

2014-01-01

Global atmospheric carbon dioxide (CO2) measurements through the Active Sensing of CO2 Emissions over Nights, Days, and Seasons (ASCENDS) Decadal Survey recommended space mission are critical for improving our understanding of CO2 sources and sinks. IM-CW (Intensity Modulated Continuous Wave) lidar techniques are investigated as a means of facilitating CO2 measurements from space to meet the ASCENDS science requirements. In previous laboratory and flight experiments we have successfully used linear swept frequency modulation to discriminate surface lidar returns from intermediate aerosol and cloud contamination. Furthermore, high accuracy and precision ranging to the surface as well as to the top of intermediate clouds, which is a requirement for the inversion of the CO2 column-mixing ratio from the instrument optical depth measurements, has been demonstrated with the linear swept frequency modulation technique. We are concurrently investigating advanced techniques to help improve the auto-correlation properties of the transmitted waveform implemented through physical hardware to make cloud rejection more robust in special restricted scenarios. Several different carrier based modulation techniques are compared including orthogonal linear swept, orthogonal non-linear swept, and Binary Phase Shift Keying (BPSK). Techniques are investigated that reduce or eliminate sidelobes. These techniques have excellent auto-correlation properties while possessing a finite bandwidth (by way of a new cyclic digital filter), which will reduce bias error in the presence of multiple scatterers. Our analyses show that the studied modulation techniques can increase the accuracy of CO2 column measurements from space. A comparison of various properties such as signal to noise ratio (SNR) and time-bandwidth product are discussed.

Detection of oral early cancerous lesion by using polarization-sensitive optical coherence tomography: mice model

NASA Astrophysics Data System (ADS)

Lee, Hong-Yi; Chen, Ping-Hsien; Lee, Tzu-Han; Chang, Kuo-Wei; Kuo, Wen-Chuan

2018-02-01

Oral cancer is the 11th most common cancer worldwide, especially in a male adult. The median age of death in oral cancer was 55 years, 10-20 years earlier than other cancers. Presently, oral cancer is often found in late stage, because the lesion is often flat in early stage and is difficult to diagnose under traditional white light imaging. The only definitive method for determining cancer is an invasive biopsy and then using histology examination. How to detect precancerous lesions or early malignant lesions is an important issue for improving prognosis of oral cancer. Optical coherence tomography (OCT) is a new optical tool for diagnosing early malignant lesions in the skin or gastrointestinal tract recently. Here we report a new method for detecting precancerous or early malignant oral lesions by using swept source polarization-sensitive optical coherence tomography (PS-OCT) with center-wavelength 1310 nm, bandwidth 110 nm and 100 kHz swept rate. We used all single-mode fiber design to detect the change of birefringence information in the epithelium structure. This system has an advantage that enables measurement of backscattered intensity and birefringence simultaneously with only one A-scan per transverse location. In preliminary result, we computed the slope of the every A-scan signal in tissue part using a linear-curve fitting in backscattered intensity and birefringence on the enface. In this research, we used an oral cancer mice model for observing the change of structure and birefringence properties in different stages of oral cancer mice. We presented the parametric enface imaging that can detect the early oral malignant lesions.

Macular hole-associated retinal detachment in Best vitelliform dystrophy: Series of two cases and literature review

PubMed Central

Tewari, Ruchir; Kumar, Vinod; Ravani, Raghav; Dubey, Devashish; Chandra, Parijat; Kumar, Atul

2018-01-01

Two eyes of 2 patients with macular hole-associated retinal detachment in clinically diagnosed vitelliruptive stage of Best vitelliform dystrophy were surgically managed by 25-gauge sutureless pars plana vitrectomy, internal limiting membrane (ILM) peeling with inverted ILM flap, and short-acting (SF6) gas tamponade. The patients were assessed with respect to best-corrected visual acuity, color fundus photographs, shortwave fundus autofluorescence, and swept source optical coherence tomography. Surgical intervention led to Type 1 closure of macular hole, resolution of retinal detachment, and improvement in vision in both patients. PMID:29676326

Laser optomechanics

NASA Astrophysics Data System (ADS)

Yang, Weijian; Adair Gerke, Stephen; Wei Ng, Kar; Rao, Yi; Chase, Christopher; Chang-Hasnain, Connie J.

2015-09-01

Cavity optomechanics explores the interaction between optical field and mechanical motion. So far, this interaction has relied on the detuning between a passive optical resonator and an external pump laser. Here, we report a new scheme with mutual coupling between a mechanical oscillator supporting the mirror of a laser and the optical field generated by the laser itself. The optically active cavity greatly enhances the light-matter energy transfer. In this work, we use an electrically-pumped vertical-cavity surface-emitting laser (VCSEL) with an ultra-light-weight (130 pg) high-contrast-grating (HCG) mirror, whose reflectivity spectrum is designed to facilitate strong optomechanical coupling, to demonstrate optomechanically-induced regenerative oscillation of the laser optomechanical cavity. We observe >550 nm self-oscillation amplitude of the micromechanical oscillator, two to three orders of magnitude larger than typical, and correspondingly a 23 nm laser wavelength sweep. In addition to its immediate applications as a high-speed wavelength-swept source, this scheme also offers a new approach for integrated on-chip sensors.

Noninvasive imaging of oral mucosae with optical coherence tomography

NASA Astrophysics Data System (ADS)

Lee, Cheng-Yu; Chen, Wei-Chuan; Tsai, Meng-Tsan

2017-04-01

In this study, a swept-source optical coherence tomography (OCT) system is developed for in vivo visualization of structural and vascular morphology oral mucosa. For simplification of optical probe fabrication, probe weight, and system setup, the body of the scanning probe is fabricated by a 3D printer to fix the optical components and the mechanical scanning device, and a partially reflective slide is attached at the output end of probe to achieve a common-path configuration. Aside from providing the ability of 3D structural imaging with the developed system, 3D vascular images of oral mucosa can be simultaneously obtained. Then, different locations of oral mucosa are scanned with common-path OCT. The results show that epithelium and lamina propria layers as well as fungiform papilla can be identified and microvascular images can be acquired. With the proposed probe, the system cost and volume can be greatly reduced. Experimental results indicate that such common-path OCT system could be further implemented for oral cancer diagnosis.

Visualization of hair follicles using high-speed optical coherence tomography based on a Fourier domain mode locking laser

NASA Astrophysics Data System (ADS)

Tsai, M.-T.; Chang, F.-Y.

2012-04-01

In this study, a swept-source optical coherence tomography (SS-OCT) system with a Fourier domain mode locking (FDML) laser is proposed for a dermatology study. The homemade FDML laser is one kind of frequency-sweeping light source, which can provide output power of >20 mW and an output spectrum of 65 nm in bandwidth centered at 1300 nm, enabling imaging with an axial resolution of 12 μm in the OCT system. To eliminate the forward scans from the laser output and insert the delayed backward scans, a Mach-Zehnder configuration is implemented. Compared with conventional frequency-sweeping light sources, the FDML laser can achieve much higher scan rates, as high as ˜240 kHz, which can provide a three-dimensional imaging rate of 4 volumes/s. Furthermore, the proposed high-speed SS-OCT system can provide three-dimensional (3D) images with reduced motion artifacts. Finally, a high-speed SS-OCT system is used to visualize hair follicles, demonstrating the potential of this technology as a tool for noninvasive diagnosis of alopecia.

Handheld ultrahigh speed swept source optical coherence tomography instrument using a MEMS scanning mirror.

PubMed

Lu, Chen D; Kraus, Martin F; Potsaid, Benjamin; Liu, Jonathan J; Choi, Woojhon; Jayaraman, Vijaysekhar; Cable, Alex E; Hornegger, Joachim; Duker, Jay S; Fujimoto, James G

2013-12-20

We developed an ultrahigh speed, handheld swept source optical coherence tomography (SS-OCT) ophthalmic instrument using a 2D MEMS mirror. A vertical cavity surface-emitting laser (VCSEL) operating at 1060 nm center wavelength yielded a 350 kHz axial scan rate and 10 µm axial resolution in tissue. The long coherence length of the VCSEL enabled a 3.08 mm imaging range with minimal sensitivity roll-off in tissue. Two different designs with identical optical components were tested to evaluate handheld OCT ergonomics. An iris camera aided in alignment of the OCT beam through the pupil and a manual fixation light selected the imaging region on the retina. Volumetric and high definition scans were obtained from 5 undilated normal subjects. Volumetric OCT data was acquired by scanning the 2.4 mm diameter 2D MEMS mirror sinusoidally in the fast direction and linearly in the orthogonal slow direction. A second volumetric sinusoidal scan was obtained in the orthogonal direction and the two volumes were processed with a software algorithm to generate a merged motion-corrected volume. Motion-corrected standard 6 x 6 mm(2) and wide field 10 x 10 mm(2) volumetric OCT data were generated using two volumetric scans, each obtained in 1.4 seconds. High definition 10 mm and 6 mm B-scans were obtained by averaging and registering 25 B-scans obtained over the same position in 0.57 seconds. One of the advantages of volumetric OCT data is the generation of en face OCT images with arbitrary cross sectional B-scans registered to fundus features. This technology should enable screening applications to identify early retinal disease, before irreversible vision impairment or loss occurs. Handheld OCT technology also promises to enable applications in a wide range of settings outside of the traditional ophthalmology or optometry clinics including pediatrics, intraoperative, primary care, developing countries, and military medicine.

Cross-sectional imaging of extracted jawbone of a pig by optical coherence tomography

NASA Astrophysics Data System (ADS)

Tachikawa, Noriko; Yoshimura, Reiko; Ohbayashi, Kohji

2011-03-01

Dental implantation has become popular in dental treatments. Although careful planning is made to identify vital structures such as the inferior alveolar nerve or the sinus, as well as dimensions of the bone, prior to commencement of surgery, dental implantation is not fully free from risks. If a diagnostic tool is available to objectively measure bone feature before surgery and dimensions during surgery, considerable fraction of the risks may be avoided. Optical coherence tomography (OCT) is a candidate for the purpose, which enables cross-sectional imaging of bone. In this work, we performed in vitro cross-sectional imaging of extracted pig's jawbone with swept source OCT using superstructure-grating distributed Bragg reflector (SSG-DBR) laser as the source. The relatively long wavelength range of 1600nm of the laser is suitable for deeper bone imaging. We confirmed an image penetration depth of about 3 mm in physical length, which satisfies one of the criterions to apply OCT for in vivo diagnosis of bone during surgery.

Single-mode 140 nm swept light source realized by using SSG-DBR lasers

NASA Astrophysics Data System (ADS)

Fujiwara, N.; Yoshimura, R.; Kato, K.; Ishii, H.; Kano, F.; Kawaguchi, Y.; Kondo, Y.; Ohbayashi, K.; Oohashi, H.

2008-02-01

We demonstrate a single-mode and fast wavelength swept light source by using Superestrucuture grating distributed Bragg reflector (SSG-DBR) lasers for use in optical frequency-domain reflectometry optical coherence tomography. The SSG-DBR lasers provide single-mode operation resulting in high coherency. Response of the wavelength tuning is very fast; several nanoseconds, but there was an unintentional wavelength drift resulting from a thermal drift due to injecting tuning current. The dri1ft unfortunately requires long time to converge; more than a few milliseconds. For suppressing the wavelength drift, we introduced Thermal Drift Compensation mesa (TDC) parallel to the laser mesa with the spacing of 20 μm. By controlling TDC current to satisfy the total electric power injected into both the laser mesa and the TDC mesa, the thermal drift can be suppressed. In the present work, we fabricated 4 wavelength's kinds of SSG-DBR laser, which covers respective wavelength band; S-band (1496-1529 nm), C-band (1529-1564 nm), L --band (1564-1601 nm), and L +-band (1601-1639). We set the frequency channel of each laser with the spacing 6.25 GHz and 700 channels. The total frequency channel number is 2800 channels (700 ch × 4 lasers). We simultaneously operated the 4 lasers with a time interval of 500 ns/channel. A wavelength tuning range of more than 140 nm was achieved within 350 μs. The output power was controlled to be 10 mW for all channels. A single-mode, accurate, wide, and fast wavelength sweep was demonstrated with the SSG-DBR lasers having TDC mesa structure for the first time.

Diagnostic Ability of Wide-field Retinal Nerve Fiber Layer Maps Using Swept-Source Optical Coherence Tomography for Detection of Preperimetric and Early Perimetric Glaucoma.

PubMed

Lee, Won June; Na, Kyeong Ik; Kim, Young Kook; Jeoung, Jin Wook; Park, Ki Ho

2017-06-01

To evaluate the diagnostic ability of wide-field retinal nerve fiber layer (RNFL) maps with swept-source optical coherence tomography (SS-OCT) for detection of preperimetric (PPG) and early perimetric glaucoma (EG). One hundred eighty-four eyes, including 67 healthy eyes, 43 eyes with PPG, and 74 eyes with EG, were analyzed. Patients underwent a comprehensive ocular examination including red-free RNFL photography, visual field testing and wide-field SS-OCT scanning (DRI-OCT-1 Atlantis; Topcon, Tokyo, Japan). SS-OCT provides a wide-field RNFL thickness map and a SuperPixel map, which are composed of the RNFL deviation map of the peripapillary area and the deviation map of the composition of the ganglion cell layer with the inner plexiform layer and RNFL (GC-IPL+RNFL) in the macular area. The ability to discriminate PPG and EG from healthy eyes was assessed using sensitivity, specificity, and area under the receiver operating characteristic curve (AUC) for all parameters and criteria provided by the wide-field SS-OCT scan. The wide-field RNFL thickness map using SS-OCT showed the highest sensitivity of PPG-diagnostic and EG-diagnostic performance compared with the other SS-OCT criteria based on the internal normative base (93.0 and 97.3%, respectively). Among the SS-OCT continuous parameters, the RFNL thickness of the 7 clock-hour, inferior and inferotemporal macular ganglion cell analyses showed the largest AUC of PPG-diagnostic and EG-diagnostic performance (AUC=0.809 to 0.865). The wide-field RNFL thickness map using SS-OCT performed well in distinguishing eyes with PPG and EG from healthy eyes. In the clinical setting, wide-field RNFL maps of SS-OCT can be useful tools for detection of early-stage glaucoma.

In vivo crystalline lens measurements with novel swept-source optical coherent tomography: an investigation on variability of measurement.

PubMed

Shoji, Takuhei; Kato, Naoko; Ishikawa, Sho; Ibuki, Hisashi; Yamada, Norihiro; Kimura, Itaru; Shinoda, Kei

2017-01-01

To evaluate the reproducibility of in vivo crystalline lens measurements obtained by novel commercially available swept-source (SS) optical coherence tomography (OCT) specifically designed for anterior segment imaging. One eye from each of 30 healthy subjects was randomly selected using the CASIA2 (Tomey, Nagoya, Japan) in two separate visits within a week. Each eye was imaged twice. After image scanning, the anterior and posterior lens curvatures and lens thickness were calculated automatically by the CASIA2 built-in program at 0 dioptre (D) (static), -1 D, -3 D and -5 D accommodative stress. The intraobserver and intervisit reproducibility coefficient (RC) and intraclass correlation coefficient (ICC) were calculated. The intraobserver and intervisit RCs ranged from 0.824 to 1.254 mm and 0.789 to 0.911 mm for anterior lens curvature, from 0.276 to 0.299 mm and 0.221 to 0.270 mm for posterior lens curvature and from 0.065 to 0.094 mm and 0.054 to 0.132 mm for lens thickness, respectively. The intraobserver and intervisit ICCs ranged from 0.831 to 0.865 and 0.828 to 0.914 for anterior lens curvature, from 0.832 to 0.898 and 0.840 to 0.933 for posterior lens curvature and from 0.980 to 0.992 and 0.942 to 0.995 for lens thickness. High ICC values were observed for each measurement regardless of accommodative stress. RCs in younger subjects tended to be larger than those in older subjects. This novel anterior segment SS-OCT instrument produced reliable in vivo crystalline lens measurement with good repeatability and reproducibility regardless of accommodation stress.

Comparison of microleakage of Scotchbond™ Universal Adhesive with methacrylate resin in Class V restorations by two methods: Swept source optical coherence tomography and dye penetration

PubMed Central

Kermanshah, Hamid; Khorsandian, Hossein

2017-01-01

Background: One of the most important factors in restoration failure is microleakage at the restoration interface. Furthermore, a new generation of bonding, Scotchbond Universal (multi-mode adhesive), has been introduced to facilitate the bonding steps. The aim of this study was to compare the microleakage of Class V cavities restored using Scotchbond™ Universal with Scotchbond Multi-Purpose in two procedures. Materials and Methods: Eighteen freshly extracted human molars were used in this study. Thirty-six standardized Class V cavities were prepared on the buccal and lingual surfaces. The teeth were divided into three groups: (1) Group A: Scotchbond Universal with “self-etching” procedure and nanohybrid composite Filtek Z350. (2) Group B: Scotchbond Universal with “total etching” procedure and Filtek Z350. (3) Group C: Scotchbond Multi-Purpose and Filtek Z350. Microleakage at enamel and dentinal margins was evaluated after thermocycling under 5000 cycles by two methods of microleakage assay: swept source optical coherence tomography (OCT) and dye penetration. Wilcoxon's signed-rank test and Kruskal–Wallis test were used to analyze microleakage. Results: In silver nitrate dye penetration method, group A exhibited the minimum microleakage at dentin margins and group C exhibited the minimum microleakage at enamel margins (P < 0.05). Furthermore, in OCT method, group C demonstrated the minimum microleakage at enamel margins (P = 0.047), with no difference in the microleakage rate at dentin margins. Conclusion: Scotchbond Universal with “self-etching” procedure at dentin margin exhibited more acceptable performance compared to the Scotchbond Multi-Purpose with the two methods. PMID:28928782

Usability and reproducibility of tear meniscus values generated via swept-source optical coherence tomography and the slit lamp with a graticule method.

PubMed

Imamura, Hitoshi; Tabuchi, Hitoshi; Nakakura, Shunsuke; Nagasato, Daisuke; Baba, Hiroaki; Kiuchi, Yoshiaki

2018-04-01

To investigate the usability and the reproducibility of the tear meniscus values via swept-source optical coherence tomography (SS-OCT) and the conventional slit lamp microscope method with a graticule. The right eye was examined in 90 healthy adult subjects who were grouped according to age (group 1: 20-39 years; group 2: 40-59 years; group 3: ≥60 years). The tear meniscus height (TMH) and tear meniscus area were measured using SS-OCT and TMH by the slit lamp microscope method. The reproducibility of each method was calculated using intraclass correlation coefficients (ICCs) in additionally enrolled 30 healthy young subjects. We also evaluated TMH at 3 mm from the corneal center in both temporal and nasal directions using SS-OCT. The mean of the TMH values measured by SS-OCT was significantly higher than those measured by the slit lamp method (328 vs. 212 μm, P < 0.001, respectively). High reproducibility was observed for each method (ICC > 0.75 for both). No statistically significant differences were found in TMH among the age groups using both SS-OCT and slit lamp methods (P = 0.985, 0.380, respectively). TMH values at both sides of the corneal center were significantly smaller than those at the corneal center (P < 0.0001). TMH values obtained by the slit lamp method were lower than those obtained by SS-OCT. However, both methods yielded highly reproducible TMH measurements, suggesting that they are clinically useful. Tear meniscus values did not vary by age but by measurement points in our cohort.

Three-Dimensional Enhanced Imaging of Vitreoretinal Interface in Diabetic Retinopathy Using Swept-Source Optical Coherence Tomography.

PubMed

Adhi, Mehreen; Badaro, Emmerson; Liu, Jonathan J; Kraus, Martin F; Baumal, Caroline R; Witkin, Andre J; Hornegger, Joachim; Fujimoto, James G; Duker, Jay S; Waheed, Nadia K

2016-02-01

To analyze the vitreoretinal interface in diabetic eyes using 3-dimensional wide-field volumes acquired using high-speed, long-wavelength swept-source optical coherence tomography (SSOCT). Prospective cross-sectional study. Fifty-six diabetic patients (88 eyes) and 11 healthy nondiabetic controls (22 eyes) were recruited. Up to 8 SSOCT volumes were acquired for each eye. A registration algorithm removed motion artifacts and merged multiple SSOCT volumes to improve signal. Vitreous visualization was enhanced using vitreous windowing method. Of 88 diabetic eyes, 20 eyes had no retinopathy, 21 eyes had nonproliferative diabetic retinopathy (NPDR) without macular edema, 20 eyes had proliferative diabetic retinopathy (PDR) without macular edema, and 27 eyes had diabetic macular edema (DME) with either NPDR or PDR. Thick posterior hyaloid relative to healthy nondiabetic controls was observed in 0 of 20 (0%) diabetic eyes without retinopathy, 4 of 21 (19%) eyes with NPDR, 11 of 20 (55%) eyes with PDR, and 11 of 27 (41%) eyes with DME (P = .0001). Vitreoschisis was observed in 6 of 22 (27%) healthy nondiabetic eyes, 9 of 20 (45%) diabetic eyes without retinopathy, 10 of 21 (48%) eyes with NPDR, 13 of 20 (65%) eyes with PDR, and 17 of 27 (63%) eyes with DME (P = .007). While no healthy nondiabetic controls and diabetic eyes without retinopathy had adhesions/pegs between detached posterior hyaloid and retina, 1 of 21 (4%), 11 of 20 (55%), and 11 of 27 (41%) eyes with NPDR, PDR, and DME, respectively, demonstrated this feature (P = .0001). SSOCT with motion-correction and vitreous windowing provides wide-field 3-dimensional information of vitreoretinal interface in diabetic eyes. This may be useful in assessing progression of retinopathy, planning diabetic vitreous surgery, and predicting treatment outcomes. Copyright © 2016 Elsevier Inc. All rights reserved.

Fractography of interface after microtensile bond strength test using swept-source optical coherence tomography.

PubMed

Dao Luong, Minh Nguyet; Shimada, Yasushi; Turkistani, Alaa; Tagami, Junji; Sumi, Yasunori; Sadr, Alireza

2016-07-01

To determine the effect of crosshead speed and placement technique on interfacial crack formation in microtensile bond strength (MTBS) test using swept-source optical coherence tomography (SS-OCT). MTBS test beams (0.9×0.9mm(2)) were prepared from flat human dentin disks bonded with self-etch adhesive (Clearfil SE Bond, Kuraray) and universal composite (Clearfil AP-X, Kuraray) with or without flowable composite lining (Estelite Flow Quick, Tokuyama). Each beam was scanned under SS-OCT (Santec, Japan) at 1319nm center wavelength before MTBS test was performed at crosshead speed of either 1 or 10mm/min (n=10). The beams were scanned by SS-OCT again to detect and measure cracks at the debonded interface using digital image analysis software. Representative beams were observed under confocal laser scanning microscope to confirm the fractography findings. Two-way ANOVA showed that for MTBS the crosshead speed was not a significant factor (p>0.05), while there was a difference between placement techniques (p<0.001), with flowable lining yielding higher mean values. On the other hand, for crack formation, there was a significant difference between crosshead speeds (p<0.01), while the placement technique did not show up as a statistically significant factor (p>0.05). The interaction of factors were not significant (p>0.05). Testing MTBS samples at higher crosshead speeds induced more cracks in dentin. Lining with a flowable composite improved the bonding quality and increased the bond strength. SS-OCT can visualize interfacial cracks after restoration debonding. Copyright © 2016 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

Three-Dimensional Morphometric Analysis of the Iris by Swept-Source Anterior Segment Optical Coherence Tomography in a Caucasian Population.

PubMed

Invernizzi, Alessandro; Giardini, Piero; Cigada, Mario; Viola, Francesco; Staurenghi, Giovanni

2015-07-01

We analyzed by swept-source anterior segment optical coherence tomography (SS-ASOCT) the three-dimensional iris morphology in a Caucasian population, and correlated the findings with iris color, iris sectors, subject age, and sex. One eye each from consecutive healthy emmetropic (refractive spherical equivalent ± 3 diopters) volunteers were selected for the study. The enrolled eye underwent standardized anterior segment photography to assess iris color. Iris images were assessed by SS-ASOCT for volume, thickness, width, and pupil size. Sectoral variations of morphometric data among the superior, nasal, inferior, and temporal sectors were recorded. A total of 135 eyes from 57 males and 78 females, age 49 ± 17 years, fulfilled the inclusion criteria. All iris morphometric parameters varied significantly among the different sectors (all P < 0.0001). Iris total volume and thickness were significantly correlated with increasingly darker pigmentation (P < 0.0001, P = 0.0384, respectively). Neither width nor pupil diameter was influenced by iris color. Age did not affect iris volume or thickness; iris width increased and pupil diameter decreased with age (rs = 0.52, rs = -0.58, respectively). There was no effect of sex on iris volume, thickness, or pupil diameter; iris width was significantly greater in males (P = 0.007). Morphology of the iris varied by iris sector, and iris color was associated with differences in iris volume and thickness. Morphological parameter variations associated with iris color, sector, age, and sex can be used to identify pathological changes in suspect eyes. To be effective in clinical settings, construction of iris morphological databases for different ethnic and racial populations is essential.

Association of iris surface features with iris parameters assessed by swept-source optical coherence tomography in Asian eyes.

PubMed

Tun, Tin A; Chua, Jacqueline; Shi, Yuan; Sidhartha, Elizabeth; Thakku, Sri Gowtham; Shei, William; Tan, Marcus Chiang Lee; Quah, Joanne Hui Min; Aung, Tin; Cheng, Ching-Yu

2016-12-01

To characterise the association of iris surface features (crypts, furrows and colour) with iris volume and curvature assessed by swept-source optical coherence tomography (SSOCT) in Asian eyes. Iris crypts (by number and size) and furrows (by number and circumferential extent) were graded from iris photographs. Iris colour was measured by a customised algorithm written on MATLAB (MathWorks, Natick, Massachusetts, USA). The iris was imaged by SSOCT (SS-1000, CASIA, Tomey, Nagoya, Japan). The associations of surface features with iris parameters were analysed using a generalised estimating equation. A total of 1704 subjects (3297 eyes) were included in the analysis. The majority was Chinese (86.4%), and 63.2% were females, and their mean age (±SD) was 61.4±6.6 years. After adjusting for age, sex, ethnicity, pupil size and corneal arcus, higher iris crypt grade was independently associated with smaller iris volume (β=-0.54, p<0.001), whereas darker irides and higher iris furrow grade were associated with larger iris volume (β=-0.041, p<0.001) and (β=0.233, p<0.001), respectively. Lighter coloured irides with more crypts and/or more furrows were also associated with less convexity (crypts: β=-0.003, p=0.03; furrows: β=-0.004, p=0.007; and colour: β=-0.001, p=0.005). Iris surface features were highly correlated with iris volume and curvature. Irides with more crypts have a smaller volume; and darker irides with more furrows have a larger volume. Lighter irides with more crypts and/or furrows have less convexity. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

In vivo crystalline lens measurements with novel swept-source optical coherent tomography: an investigation on variability of measurement

PubMed Central

Shoji, Takuhei; Kato, Naoko; Ishikawa, Sho; Ibuki, Hisashi; Yamada, Norihiro; Kimura, Itaru; Shinoda, Kei

2017-01-01

Objective To evaluate the reproducibility of in vivo crystalline lens measurements obtained by novel commercially available swept-source (SS) optical coherence tomography (OCT) specifically designed for anterior segment imaging. Methods and analysis One eye from each of 30 healthy subjects was randomly selected using the CASIA2 (Tomey, Nagoya, Japan) in two separate visits within a week. Each eye was imaged twice. After image scanning, the anterior and posterior lens curvatures and lens thickness were calculated automatically by the CASIA2 built-in program at 0 dioptre (D) (static), −1 D, −3 D and −5 D accommodative stress. The intraobserver and intervisit reproducibility coefficient (RC) and intraclass correlation coefficient (ICC) were calculated. Results The intraobserver and intervisit RCs ranged from 0.824 to 1.254 mm and 0.789 to 0.911 mm for anterior lens curvature, from 0.276 to 0.299 mm and 0.221 to 0.270 mm for posterior lens curvature and from 0.065 to 0.094 mm and 0.054 to 0.132 mm for lens thickness, respectively. The intraobserver and intervisit ICCs ranged from 0.831 to 0.865 and 0.828 to 0.914 for anterior lens curvature, from 0.832 to 0.898 and 0.840 to 0.933 for posterior lens curvature and from 0.980 to 0.992 and 0.942 to 0.995 for lens thickness. High ICC values were observed for each measurement regardless of accommodative stress. RCs in younger subjects tended to be larger than those in older subjects. Conclusions This novel anterior segment SS-OCT instrument produced reliable in vivo crystalline lens measurement with good repeatability and reproducibility regardless of accommodation stress. PMID:29354706

Reliability and repeatability of swept-source Fourier-domain optical coherence tomography and Scheimpflug imaging in keratoconus.

PubMed

Szalai, Eszter; Berta, András; Hassan, Ziad; Módis, László

2012-03-01

To evaluate the repeatability and reliability of a recently introduced swept-source Fourier-domain anterior segment optical coherence tomography (AS-OCT) system and a high-resolution Scheimpflug camera and to assess the agreement between the 2 instruments when measuring healthy eyes and eyes with keratoconus. Department of Ophthalmology, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary. Evaluation of diagnostic test or technology. Three consecutive series of anterior segment images were taken with AS-OCT (Casia SS-1000) followed by rotating Scheimpflug imaging (Pentacam high resolution). Axial keratometry in the steep and flat meridians and astigmatism values were recorded. Pachymetry in the apex, center, and the thinnest position and anterior chamber depth (ACD) measurements were also taken. This study enrolled 57 healthy volunteers (57 eyes) and 56 patients (84 eyes) with keratoconus. Significant difference was found in all measured anterior segment parameters between normal eyes and keratoconic eyes (P<.05). In keratoconic eyes, the difference between repeated measurements was less with AS-OCT than with Scheimpflug imaging in every keratometry and astigmatism value, in apical thickness, and in ACD. For keratometry, the thinnest and central pachymetry measurement repeatability was better in healthy eyes than in keratoconic eyes with both instruments. In general, the mean difference between AS-OCT and Scheimpflug imaging was higher in cases of keratoconus. Significant differences in keratometry, pachymetry, and ACD results were found between AS-OCT and Scheimpflug imaging. However, the repeatability of the measurements was comparable. No author has a financial or proprietary interest in any material or method mentioned. Copyright © 2012 ASCRS and ESCRS. Published by Elsevier Inc. All rights reserved.

In vivo imaging of human labial glands using advanced optical coherence tomography.

PubMed

Ozawa, Nobuyoshi; Sumi, Yasunori; Shimozato, Kazuo; Chong, Changho; Kurabayashi, Tohru

2009-09-01

Optical coherence tomography (OCT) has emerged as a high-resolution noninvasive clinical imaging application. The purpose of this study was to show OCT images of human labial glands obtained using a swept-source (SS) OCT system. Labial gland OCT imaging was carried out using our new SS-OCT system for 5 healthy volunteers using a hand-held in vivo OCT scanning probe. The labial tissue was scanned in a superior to inferior direction in 2 and 3 dimensions. The resulting 2- and 3-dimensional ultrahigh-resolution images of in vivo OCT human labial minor salivary glands revealed the epithelium, connective tissue, lobes, and duct. OCT was capable of providing simultaneous and noninvasive structural information with high resolution. This clinical imaging modality promises to have clinical impact in the diagnosis of such conditions as Sjögren syndrome and xerostomia.

Large depth high-precision FMCW tomography using a distributed feedback laser array

NASA Astrophysics Data System (ADS)

DiLazaro, Thomas; Nehmetallah, George

2018-02-01

Swept-source optical coherence tomography (SS-OCT) has been widely employed in the medical industry for the high resolution imaging of subsurface biological structures. SS-OCT typically exhibits axial resolutions on the order of tens of microns at speeds of hundreds of kilohertz. Using the same coherent heterodyne detection technique, frequency modulated continuous wave (FMCW) ladar has been used for highly precise ranging for distances up to kilometers. Distributed feedback lasers (DFBs) have been used as a simple and inexpensive source for FMCW ranging. Here, we use a bandwidth-combined DFB array for sub-surface volume imaging at a 27 μm axial resolution over meters of distance. 2D and 3D tomographic images of several semi-transparent and diffuse objects at distances up to 10 m will be presented.

In utero mouse embryonic imaging with OCT for ophthalmologic research

NASA Astrophysics Data System (ADS)

Syed, Saba H.; Larina, Irina V.; Dickinson, Mary E.; Larin, Kirill V.

2011-03-01

Live imaging of an eye during embryonic development in mammalian model is important for understanding dynamic aspects of normal and abnormal eye morphogenesis. In this study, we used Swept Source Optical Coherence Tomography (SS-OCT) for live structural imaging of mouse embryonic eye through the uterine wall. The eye structure was reconstructed in mouse embryos at 13.5 to 17.5 days post coitus (dpc). Despite the limited imaging depth of OCT in turbid tissues, we were able to visualize the whole eye globe at these stages. These results suggest that live in utero OCT imaging is a useful tool to study embryonic eye development in the mouse model.

Active depth-guiding handheld micro-forceps for membranectomy based on CP-SSOCT

NASA Astrophysics Data System (ADS)

Cheon, Gyeong Woo; Lee, Phillip; Gonenc, Berk; Gehlbach, Peter L.; Kang, Jin U.

2016-03-01

In this study, we demonstrate a handheld motion-compensated micro-forceps system using common-path swept source optical coherence tomography with highly accurate depth-targeting and depth-locking for Epiretinal Membrane Peeling. Two motors and a touch sensor were used to separate the two independent motions: motion compensation and tool-tip manipulation. A smart motion monitoring and guiding algorithm was devised for precise and intuitive freehand control. Ex-vivo bovine eye experiments were performed to evaluate accuracy in a bovine retina retinal membrane peeling model. The evaluation demonstrates system capabilities of 40 um accuracy when peeling the epithelial layer of bovine retina.

True logarithmic amplification of frequency clock in SS-OCT for calibration

PubMed Central

Liu, Bin; Azimi, Ehsan; Brezinski, Mark E.

2011-01-01

With swept source optical coherence tomography (SS-OCT), imprecise signal calibration prevents optimal imaging of biological tissues such as coronary artery. This work demonstrates an approach using a true logarithmic amplifier to precondition the clock signal, with the effort to minimize the noises and phase errors for optimal calibration. This method was validated and tested with a high-speed SS-OCT. The experimental results manifest its superior ability on optimization of the calibration and improvement of the imaging performance. Particularly, this hardware-based approach is suitable for real-time calibration in a high-speed system where computation time is constrained. PMID:21698036

Detection of Silent Type I Choroidal Neovascular Membrane in Chronic Central Serous Chorioretinopathy Using En Face Swept-Source Optical Coherence Tomography Angiography.

PubMed

Moussa, Magdy; Leila, Mahmoud; Khalid, Hagar; Lolah, Mohamed

2017-01-01

To evaluate the efficacy of SS-OCTA in the detection of silent CNV secondary to chronic CSCR compared to that of FFA and SS-OCT. A retrospective observational case series reviewing the clinical data, FFA, SS-OCT, and SS-OCTA images of patients with chronic CSCR, and comparing the findings. SS-OCTA detects the CNV complex and delineates it from the surrounding pathological features of chronic CSCR by utilizing the blood flow detection algorithm, OCTARA, and the ultrahigh-definition B-scan images of the retinal microstructure generated by swept-source technology. The bivariate correlation procedure was used for the calculation of the correlation matrix of the variables tested. The study included 60 eyes of 40 patients. Mean age was 47.6 years. Mean disease duration was 14.5 months. SS-OCTA detected type 1 CNV in 5 eyes (8.3%). In all 5 eyes, FFA and SS-OCT were inconclusive for CNV. The presence of foveal thinning, opaque material beneath irregular flat PED, and increased choroidal thickness in chronic CSCR constitutes a high-risk profile for progression to CNV development. Silent type 1 CNV is an established complication of chronic CSCR. SS-OCTA is indispensable in excluding CNV especially in high-risk patients and whenever FFA and SS-OCT are inconclusive.

Doppler optical coherence microscopy and tomography applied to inner ear mechanics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Page, Scott; Freeman, Dennis M.; Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts

While it is clear that cochlear traveling waves underlie the extraordinary sensitivity, frequency selectivity, and dynamic range of mammalian hearing, the underlying micromechanical mechanisms remain unresolved. Recent advances in low coherence measurement techniques show promise over traditional laser Doppler vibrometry and video microscopy, which are limited by low reflectivities of cochlear structures and restricted optical access. Doppler optical coherence tomography (DOCT) and Doppler optical coherence microscopy (DOCM) both utilize a broadband source to limit constructive interference of scattered light to a small axial depth called a coherence gate. The coherence gate can be swept axially to image and measure sub-nanometermore » motions of cochlear structures throughout the cochlear partition. The coherence gate of DOCT is generally narrower than the confocal gate of the focusing optics, enabling increased axial resolution (typically 15 μm) within optical sections of the cochlear partition. DOCM, frequently implemented in the time domain, centers the coherence gate on the focal plane, achieving enhanced lateral and axial resolution when the confocal gate is narrower than the coherence gate. We compare these two complementary systems and demonstrate their utility in studying cellular and micromechanical mechanisms involved in mammalian hearing.« less

Fully distributed absolute blood flow velocity measurement for middle cerebral arteries using Doppler optical coherence tomography

PubMed Central

Qi, Li; Zhu, Jiang; Hancock, Aneeka M.; Dai, Cuixia; Zhang, Xuping; Frostig, Ron D.; Chen, Zhongping

2016-01-01

Doppler optical coherence tomography (DOCT) is considered one of the most promising functional imaging modalities for neuro biology research and has demonstrated the ability to quantify cerebral blood flow velocity at a high accuracy. However, the measurement of total absolute blood flow velocity (BFV) of major cerebral arteries is still a difficult problem since it is related to vessel geometry. In this paper, we present a volumetric vessel reconstruction approach that is capable of measuring the absolute BFV distributed along the entire middle cerebral artery (MCA) within a large field-of-view. The Doppler angle at each point of the MCA, representing the vessel geometry, is derived analytically by localizing the artery from pure DOCT images through vessel segmentation and skeletonization. Our approach could achieve automatic quantification of the fully distributed absolute BFV across different vessel branches. Experiments on rodents using swept-source optical coherence tomography showed that our approach was able to reveal the consequences of permanent MCA occlusion with absolute BFV measurement. PMID:26977365

Fully distributed absolute blood flow velocity measurement for middle cerebral arteries using Doppler optical coherence tomography.

PubMed

Qi, Li; Zhu, Jiang; Hancock, Aneeka M; Dai, Cuixia; Zhang, Xuping; Frostig, Ron D; Chen, Zhongping

2016-02-01

Doppler optical coherence tomography (DOCT) is considered one of the most promising functional imaging modalities for neuro biology research and has demonstrated the ability to quantify cerebral blood flow velocity at a high accuracy. However, the measurement of total absolute blood flow velocity (BFV) of major cerebral arteries is still a difficult problem since it is related to vessel geometry. In this paper, we present a volumetric vessel reconstruction approach that is capable of measuring the absolute BFV distributed along the entire middle cerebral artery (MCA) within a large field-of-view. The Doppler angle at each point of the MCA, representing the vessel geometry, is derived analytically by localizing the artery from pure DOCT images through vessel segmentation and skeletonization. Our approach could achieve automatic quantification of the fully distributed absolute BFV across different vessel branches. Experiments on rodents using swept-source optical coherence tomography showed that our approach was able to reveal the consequences of permanent MCA occlusion with absolute BFV measurement.

Biomedical sensing and imaging for the anterior segment of the eye

NASA Astrophysics Data System (ADS)

Eom, Tae Joong; Yoo, Young-Sik; Lee, Yong-Eun; Kim, Beop-Min; Joo, Choun-Ki

2015-07-01

Eye is an optical system composed briefly of cornea, lens, and retina. Ophthalmologists can diagnose status of patient's eye from information provided by optical sensors or images as well as from history taking or physical examinations. Recently, we developed a prototype of optical coherence tomography (OCT) image guided femtosecond laser cataract surgery system. The system combined a swept-source OCT and a femtosecond (fs) laser and afford the 2D and 3D structure information to increase the efficiency and safety of the cataract procedure. The OCT imaging range was extended to achieve the 3D image from the cornea to lens posterior. A prototype of OCT image guided fs laser cataract surgery system. The surgeons can plan the laser illumination range for the nuclear division and segmentation, and monitor the whole cataract surgery procedure using the real time OCT. The surgery system was demonstrated with an extracted pig eye and in vivo rabbit eye to verify the system performance and stability.

Apparatus and Method for Elimination of Polarization-Induced Fading in Fiber-optic Sensor System

NASA Technical Reports Server (NTRS)

Chan, Hon Man (Inventor); Parker, Jr., Allen R. (Inventor)

2015-01-01

The invention is an apparatus and method of eliminating polarization-induced fading in interferometric fiber-optic sensor system having a wavelength-swept laser optical signal. The interferometric return signal from the sensor arms are combined and provided to a multi-optical path detector assembly and ultimately to a data acquisition and processing unit by way of a switch that is time synchronized with the laser scan sweep cycle.

Electrostatic forward-viewing scanning probe for Doppler optical coherence tomography using a dissipative polymer catheter.

PubMed

Munce, Nigel R; Mariampillai, Adrian; Standish, Beau A; Pop, Mihaela; Anderson, Kevan J; Liu, George Y; Luk, Tim; Courtney, Brian K; Wright, Graham A; Vitkin, I Alex; Yang, Victor X D

2008-04-01

A novel flexible scanning optical probe is constructed with a finely etched optical fiber strung through a platinum coil in the lumen of a dissipative polymer. The packaged probe is 2.2 mm in diameter with a rigid length of 6mm when using a ball lens or 12 mm when scanning the fiber proximal to a gradient-index (GRIN) lens. Driven by constant high voltage (1-3 kV) at low current (< 5 microA), the probe oscillates to provide wide forward-viewing angle (13 degrees and 33 degrees with ball and GRIN lens designs, respectively) and high-frame-rate (10-140 fps) operation. Motion of the probe tip is observed with a high-speed camera and compared with theory. Optical coherence tomography (OCT) imaging with the probe is demonstrated with a wavelength-swept source laser. Images of an IR card as well as in vivo Doppler OCT images of a tadpole heart are presented. This optomechanical design offers a simple, inexpensive method to obtain a high-frame-rate forward-viewing scanning probe.

Study on the weighing system based on optical fiber Bragg grating

NASA Astrophysics Data System (ADS)

Wang, Xiaona; Yu, Qingxu; Li, Yefang

2010-10-01

The optical fiber sensor based on wavelength demodulation such as fiber Bragg grating(FBG), with merits of immunity to electromagnetic interference, low drift and high precision, has been widely used in many areas, such as structural health monitoring and smart materials, and the wavelength demodulation system was also studied widely. In the paper, a weighing system based on FBG was studied. The optical source is broadband Erbium-doped fiber ring laser with a spectral range of 1500~1600nm and optical power of 2mW; A Fabry-Perot Etalon with orientation precision of 1pm was adopted as real-time wavelength calibration for the swept laser; and multichannel high resolution simultaneous sampling card was used in the system to acquire sensing signals simultaneously, thus high-resolution and real-time calibration of sweep-wavelength can be achieved. The FBG was adhered to a cantilever beam and the Bragg wavelength was demodulated with the system. The weighing system was done after calibrated with standard weight. Experimental results show that the resolution of the weighing system is 0.5 g with a full scale of 2Kg.

The Location of the Deepest Point of the Eyeball Determines the Optic Disc Configuration.

PubMed

Kim, Yong Chan; Jung, Younhea; Park, Hae-Young Lopilly; Park, Chan Kee

2017-07-19

Tilted and rotated appearances are hallmarks of the myopic optic disc. As the eyeball grows axially, the posterior pole elongates not only globally but in a localized manner as well. In this process, the optic disc is pulled towards the deepest point of the elongated eyeball, which might result in a change in optic disc configuration. Thus, we hypothesized that analyzing the variation of posterior pole contour can play a major role in understanding optic disc configuration in myopic subjects. By analyzing consecutive images of swept source OCT coronal sections at the posterior pole, the deepest interface between Bruch's membrane and the choroid could be identified as the deepest point of the eyeball (DPE). The location and the properties of the DPE differed significantly between the 125 eyes of non-glaucomatous myopic group and the 40 eyes of non-glaucomatous emmetropic group classified based on 24 mm axial length. The results suggested that the larger disc to DPE angle and the larger disc to DPE depth strongly predicts the optic disc torsion degree and the optic disc tilt. Our findings suggest that identifying the posterior pole profile plays a major role in understanding the optic disc alterations found in myopic subjects.

Intensity Modulation Techniques for Continuous-Wave Lidar for Column CO2 Measurements

NASA Astrophysics Data System (ADS)

Campbell, J. F.; Lin, B.; Obland, M. D.; Kooi, S. A.; Fan, T. F.; Meadows, B.; Browell, E. V.; Erxleben, W. H.; McGregor, D.; Dobler, J. T.; Pal, S.; O'Dell, C.

2017-12-01

Global and regional atmospheric carbon dioxide (CO2) measurements for the NASA Active Sensing of CO2 Emissions over Nights, Days, and Seasons (ASCENDS) space mission and the Atmospheric Carbon and Transport (ACT) - America project are critical for improving our understanding of global CO2 sources and sinks. Advanced Intensity-Modulated Continuous-Wave (IM-CW) lidar techniques are investigated as a means of facilitating CO2 measurements from space and airborne platforms to meet the ASCENDS and ACT-America science measurement requirements. In recent numerical, laboratory and flight experiments we have successfully used the Binary Phase Shift Keying (BPSK) and Linear Swept Frequency modulations to uniquely discriminate surface lidar returns from intermediate aerosol and cloud returns. We demonstrate the utility of BPSK to eliminate sidelobes in the range profile as a means of making Integrated Path Differential Absorption (IPDA) column CO2 measurements in the presence of optically thin clouds, thereby eliminating bias errors caused by the clouds. Furthermore, high accuracy and precision ranging to the surface as well as to the top of intermediate cloud layers, which is a requirement for the inversion of column CO2 number density measurements to column CO2 mixing ratios, has been demonstrated using new hyperfine interpolation techniques that take advantage of the periodicity of the modulation waveforms. This approach works well for both BPSK and linear swept-frequency modulation techniques and provides very high (at sub-meter level) range resolution. We compare BPSK to linear swept frequency and introduce a new technique to eliminate sidelobes in situations from linear swept frequency where the SNR is high with results that rival BPSK. We also investigate the effects of non-linear modulators, which can in some circumstances degrade the orthogonality of the waveforms, and show how to avoid this. These techniques are used in a new data processing architecture written in the C language to support the ASCENDS CarbonHawk Experiment Simulator (ACES) and ACT-America programs.

A MHz speed wavelength sweeping for ultra-high speed FBG interrogation

NASA Astrophysics Data System (ADS)

Kim, Gyeong Hun; Lee, Hwi Don; Eom, Tae Joong; Jeong, Myung Yung; Kim, Chang-Seok

2015-09-01

We demonstrated a MHz speed wavelength-swept fiber laser based on the active mode locking (AML) technique and applied to interrogation system of an array of fiber Bragg grating (FBG) sensors. MHz speed wavelength sweeping of wavelength-swept fiber laser can be obtained by programmable frequency modulation of the semiconductor optical amplifier (SOA) without any wavelength tunable filter. Both static and dynamic strain measurement of FBG sensors were successfully characterized with high linearity of an R-square value of 0.9999 at sweeping speed of 50 kHz.

Towards combined optical coherence tomography and hyper-spectral imaging for gastrointestinal endoscopy

NASA Astrophysics Data System (ADS)

Attendu, Xavier; Crunelle, Camille; de Sivry-Houle, Martin Poinsinet; Maubois, Billie; Urbain, Joanie; Turrell, Chloe; Strupler, Mathias; Godbout, Nicolas; Boudoux, Caroline

2018-04-01

Previous works have demonstrated feasibility of combining optical coherence tomography (OCT) and hyper-spectral imaging (HSI) through a single double-clad fiber (DCF). In this proceeding we present the continued development of a system combining both modalities and capable of rapid imaging. We discuss the development of a rapidly scanning, dual-band, polygonal swept-source system which combines NIR (1260-1340 nm) and visible (450-800 nm) wavelengths. The NIR band is used for OCT imaging while visible light allows HSI. Scanning rates up to 24 kHz are reported. Furthermore, we present and discuss the fiber system used for light transport, delivery and collection, and the custom signal acquisition software. Key points include the use of a double-clad fiber coupler as well as important alignments and back-reflection management. Simultaneous and co-registered imaging with both modalities is presented in a bench-top system

Numerical study of wavelength-swept semiconductor ring lasers: the role of refractive-index nonlinearities in semiconductor optical amplifiers and implications for biomedical imaging applications.

PubMed

Bilenca, A; Yun, S H; Tearney, G J; Bouma, B E

2006-03-15

Recent results have demonstrated unprecedented wavelength-tuning speed and repetition rate performance of semiconductor ring lasers incorporating scanning filters. However, several unique operational characteristics of these lasers have not been adequately explained, and the lack of an accurate model has hindered optimization. We numerically investigated the characteristics of these sources, using a semiconductor optical amplifier (SOA) traveling-wave Langevin model, and found good agreement with experimental measurements. In particular, we explored the role of the SOA refractive-index nonlinearities in determining the intracavity frequency-shift-broadening and the emitted power dependence on scan speed and direction. Our model predicts both continuous-wave and pulse operation and shows a universal relationship between the output power of lasers that have different cavity lengths and the filter peak frequency shift per round trip, therefore revealing the advantage of short cavities for high-speed biomedical imaging.

Utilizing optical coherence tomography for CAD/CAM of indirect dental restorations

NASA Astrophysics Data System (ADS)

Chityala, Ravishankar; Vidal, Carola; Jones, Robert

Optical Coherence Tomography (OCT) has seen broad application in dentistry including early carious lesion detection and imaging defects in resin composite restorations. This study investigates expanding the clinical usefulness by investigating methods to use OCT for obtaining three-dimensional (3D) digital impressions, which can be integrated to CAD/CAM manufacturing of indirect restorations. 3D surface topography `before' and `after' a cavity preparation was acquired by an intraoral cross polarization swept source OCT (CP-OCT) system with a Micro-Electro-Mechanical System (MEMS) scanning mirror. Image registration and segmentation methods were used to digitally construct a replacement restoration that modeled the original surface morphology of a hydroxyapatite sample. After high resolution additive manufacturing (e.g. polymer 3D printing) of the replacement restoration, micro-CT imaging was performed to examine the marginal adaptation. This study establishes the protocol for further investigation of integrating OCT with CAD/CAM of indirect dental restorations.

High-speed upper-airway imaging using full-range optical coherence tomography

NASA Astrophysics Data System (ADS)

Jing, Joseph; Zhang, Jun; Loy, Anthony Chin; Wong, Brian J. F.; Chen, Zhongping

2012-11-01

Obstruction in the upper airway can often cause reductions in breathing or gas exchange efficiency and lead to rest disorders such as sleep apnea. Imaging diagnosis of the obstruction region has been accomplished using computed tomography (CT) and magnetic resonance imaging (MRI). However CT requires the use of ionizing radiation, and MRI typically requires sedation of the patient to prevent motion artifacts. Long-range optical coherence tomography (OCT) has the potential to provide high-speed three-dimensional tomographic images with high resolution and without the use of ionizing radiation. In this paper, we present work on the development of a long-range OCT endoscopic probe with 1.2 mm OD and 20 mm working distance used in conjunction with a modified Fourier domain swept source OCT system to acquire structural and anatomical datasets of the human airway. Imaging from the bottom of the larynx to the end of the nasal cavity is completed within 40 s.

Optical Inspection and Morphological Analysis of Diospyros kaki Plant Leaves for the Detection of Circular Leaf Spot Disease.

PubMed

Wijesinghe, Ruchire Eranga; Lee, Seung-Yeol; Kim, Pilun; Jung, Hee-Young; Jeon, Mansik; Kim, Jeehyun

2016-08-12

The feasibility of using the bio-photonic imaging technique to assess symptoms of circular leaf spot (CLS) disease in Diospyros kaki (persimmon) leaf samples was investigated. Leaf samples were selected from persimmon plantations and were categorized into three groups: healthy leaf samples, infected leaf samples, and healthy-looking leaf samples from infected trees. Visually non-identifiable reduction of the palisade parenchyma cell layer thickness is the main initial symptom, which occurs at the initial stage of the disease. Therefore, we established a non-destructive bio-photonic inspection method using a 1310 nm swept source optical coherence tomography (SS-OCT) system. These results confirm that this method is able to identify morphological differences between healthy leaves from infected trees and leaves from healthy and infected trees. In addition, this method has the potential to generate significant cost savings and good control of CLS disease in persimmon fields.

Optical Inspection and Morphological Analysis of Diospyros kaki Plant Leaves for the Detection of Circular Leaf Spot Disease

PubMed Central

Wijesinghe, Ruchire Eranga; Lee, Seung-Yeol; Kim, Pilun; Jung, Hee-Young; Jeon, Mansik; Kim, Jeehyun

2016-01-01

The feasibility of using the bio-photonic imaging technique to assess symptoms of circular leaf spot (CLS) disease in Diospyros kaki (persimmon) leaf samples was investigated. Leaf samples were selected from persimmon plantations and were categorized into three groups: healthy leaf samples, infected leaf samples, and healthy-looking leaf samples from infected trees. Visually non-identifiable reduction of the palisade parenchyma cell layer thickness is the main initial symptom, which occurs at the initial stage of the disease. Therefore, we established a non-destructive bio-photonic inspection method using a 1310 nm swept source optical coherence tomography (SS-OCT) system. These results confirm that this method is able to identify morphological differences between healthy leaves from infected trees and leaves from healthy and infected trees. In addition, this method has the potential to generate significant cost savings and good control of CLS disease in persimmon fields. PMID:27529250

An Optoelectronic Equivalent Narrowband Filter for High Resolution Optical Spectrum Analysis

PubMed Central

Feng, Kunpeng; Cui, Jiwen; Dang, Hong; Wu, Weidong; Sun, Xun; Jiang, Xuelin; Tan, Jiubin

2017-01-01

To achieve a narrow bandwidth optical filter with a wide swept range for new generation optical spectrum analysis (OSA) of high performance optical sensors, an optoelectronic equivalent narrowband filter (OENF) was investigated and a swept optical filter with bandwidth of several MHz and sweep range of several tens of nanometers was built using electric filters and a sweep laser as local oscillator (LO). The principle of OENF is introduced and analysis of the OENF system is presented. Two electric filters are optimized to be RBW filters for high and medium spectral resolution applications. Both simulations and experiments are conducted to verify the OENF principle and the results show that the power uncertainty is less than 1.2% and the spectral resolution can reach 6 MHz. Then, a real-time wavelength calibration system consisting of a HCN gas cell and Fabry–Pérot etalon is proposed to guarantee a wavelength accuracy of ±0.4 pm in the C-band and to reduce the influence of phase noise and nonlinear velocity of the LO sweep. Finally, OSA experiments on actual spectra of various optical sensors are conducted using the OENF system. These experimental results indicate that OENF system has an excellent capacity for the analysis of fine spectrum structures. PMID:28208624

An Optoelectronic Equivalent Narrowband Filter for High Resolution Optical Spectrum Analysis.

PubMed

Feng, Kunpeng; Cui, Jiwen; Dang, Hong; Wu, Weidong; Sun, Xun; Jiang, Xuelin; Tan, Jiubin

2017-02-10

To achieve a narrow bandwidth optical filter with a wide swept range for new generation optical spectrum analysis (OSA) of high performance optical sensors, an optoelectronic equivalent narrowband filter (OENF) was investigated and a swept optical filter with bandwidth of several MHz and sweep range of several tens of nanometers was built using electric filters and a sweep laser as local oscillator (LO). The principle of OENF is introduced and analysis of the OENF system is presented. Two electric filters are optimized to be RBW filters for high and medium spectral resolution applications. Both simulations and experiments are conducted to verify the OENF principle and the results show that the power uncertainty is less than 1.2% and the spectral resolution can reach 6 MHz. Then, a real-time wavelength calibration system consisting of a HCN gas cell and Fabry-Pérot etalon is proposed to guarantee a wavelength accuracy of ±0.4 pm in the C-band and to reduce the influence of phase noise and nonlinear velocity of the LO sweep. Finally, OSA experiments on actual spectra of various optical sensors are conducted using the OENF system. These experimental results indicate that OENF system has an excellent capacity for the analysis of fine spectrum structures.

An All-Fiber-Optic Combined System of Noncontact Photoacoustic Tomography and Optical Coherence Tomography

PubMed Central

Eom, Jonghyun; Shin, Jun Geun; Park, Soongho; Rim, Sunghwan; Lee, Byeong Ha

2016-01-01

We propose an all-fiber-based dual-modal imaging system that combines noncontact photoacoustic tomography (PAT) and optical coherence tomography (OCT). The PAT remotely measures photoacoustic (PA) signals with a 1550-nm laser on the surface of a sample by utilizing a fiber interferometer as an ultrasound detector. The fiber-based OCT, employing a swept-source laser centered at 1310 nm, shares the sample arm of the PAT system. The fiber-optic probe for the combined system was homemade with a lensed single-mode fiber (SMF) and a large-core multimode fiber (MMF). The compact and robust common probe is capable of obtaining both the PA and the OCT signals at the same position without any physical contact. Additionally, the MMF of the probe delivers the short pulses of a Nd:YAG laser to efficiently excite the PA signals. We experimentally demonstrate the feasibility of the proposed dual-modal system with a phantom made of a fishing line and a black polyethylene terephthalate fiber in a tissue mimicking solution. The all-fiber-optic system, capable of providing complementary information about absorption and scattering, has a promising potential in minimally invasive and endoscopic imaging. PMID:27213392

An All-Fiber-Optic Combined System of Noncontact Photoacoustic Tomography and Optical Coherence Tomography.

PubMed

Eom, Jonghyun; Shin, Jun Geun; Park, Soongho; Rim, Sunghwan; Lee, Byeong Ha

2016-05-20

We propose an all-fiber-based dual-modal imaging system that combines noncontact photoacoustic tomography (PAT) and optical coherence tomography (OCT). The PAT remotely measures photoacoustic (PA) signals with a 1550-nm laser on the surface of a sample by utilizing a fiber interferometer as an ultrasound detector. The fiber-based OCT, employing a swept-source laser centered at 1310 nm, shares the sample arm of the PAT system. The fiber-optic probe for the combined system was homemade with a lensed single-mode fiber (SMF) and a large-core multimode fiber (MMF). The compact and robust common probe is capable of obtaining both the PA and the OCT signals at the same position without any physical contact. Additionally, the MMF of the probe delivers the short pulses of a Nd:YAG laser to efficiently excite the PA signals. We experimentally demonstrate the feasibility of the proposed dual-modal system with a phantom made of a fishing line and a black polyethylene terephthalate fiber in a tissue mimicking solution. The all-fiber-optic system, capable of providing complementary information about absorption and scattering, has a promising potential in minimally invasive and endoscopic imaging.

Dove prism based rotating dual beam bidirectional Doppler OCT

PubMed Central

Blatter, Cedric; Coquoz, Séverine; Grajciar, Branislav; Singh, Amardeep S. G.; Bonesi, Marco; Werkmeister, René M.; Schmetterer, Leopold; Leitgeb, Rainer A.

2013-01-01

Traditional Doppler OCT is highly sensitive to motion artifacts due to the dependence on the Doppler angle. This limits its accuracy in clinical practice. To overcome this limitation, we use a bidirectional dual beam technique equipped with a novel rotating scanning scheme employing a Dove prism. The volume is probed from two distinct illumination directions with variable controlled incidence plane, allowing for reconstruction of the true flow velocity at arbitrary vessel orientations. The principle is implemented with Swept Source OCT at 1060nm with 100,000 A-Scans/s. We apply the system to resolve pulsatile retinal absolute blood velocity by performing segment scans around the optic nerve head and circumpapillary scan time series. PMID:23847742

MEMS-Based Handheld Fourier Domain Doppler Optical Coherence Tomography for Intraoperative Microvascular Anastomosis Imaging

PubMed Central

Huang, Yong; Furtmüller, Georg J.; Tong, Dedi; Zhu, Shan; Lee, W. P. Andrew; Brandacher, Gerald; Kang, Jin U.

2014-01-01

Purpose To demonstrate the feasibility of a miniature handheld optical coherence tomography (OCT) imager for real time intraoperative vascular patency evaluation in the setting of super-microsurgical vessel anastomosis. Methods A novel handheld imager Fourier domain Doppler optical coherence tomography based on a 1.3-µm central wavelength swept source for extravascular imaging was developed. The imager was minimized through the adoption of a 2.4-mm diameter microelectromechanical systems (MEMS) scanning mirror, additionally a 12.7-mm diameter lens system was designed and combined with the MEMS mirror to achieve a small form factor that optimize functionality as a handheld extravascular OCT imager. To evaluate in-vivo applicability, super-microsurgical vessel anastomosis was performed in a mouse femoral vessel cut and repair model employing conventional interrupted suture technique as well as a novel non-suture cuff technique. Vascular anastomosis patency after clinically successful repair was evaluated using the novel handheld OCT imager. Results With an adjustable lateral image field of view up to 1.5 mm by 1.5 mm, high-resolution simultaneous structural and flow imaging of the blood vessels were successfully acquired for BALB/C mouse after orthotopic hind limb transplantation using a non-suture cuff technique and BALB/C mouse after femoral artery anastomosis using a suture technique. We experimentally quantify the axial and lateral resolution of the OCT to be 12.6 µm in air and 17.5 µm respectively. The OCT has a sensitivity of 84 dB and sensitivity roll-off of 5.7 dB/mm over an imaging range of 5 mm. Imaging with a frame rate of 36 Hz for an image size of 1000(lateral)×512(axial) pixels using a 50,000 A-lines per second swept source was achieved. Quantitative vessel lumen patency, lumen narrowing and thrombosis analysis were performed based on acquired structure and Doppler images. Conclusions A miniature handheld OCT imager that can be used for intraoperative evaluation of microvascular anastomosis was successfully demonstrated. PMID:25474742

Imaging pulse wave velocity in mouse retina using swept-source OCT (Conference Presentation)

NASA Astrophysics Data System (ADS)

Song, Shaozhen; Wei, Wei; Wang, Ruikang K.

2016-03-01

Blood vessel dynamics has been a significant subject in cardiology and internal medicine, and pulse wave velocity (PWV) on artery vessels is a classic evaluation of arterial distensibility, and has never been ascertained as a cardiovascular risk marker. The aim of this study is to develop a high speed imaging technique to capture the pulsatile motion on mouse retina arteries with the ability to quantify PWV on any arterial vessels. We demonstrate a new non-invasive method to assess the vessel dynamics on mouse retina. A Swept-source optical coherence tomography (SS-OCT) system is used for imaging micro-scale blood vessel motion. The phase-stabilized SS-OCT provides a typical displacement sensitivity of 20 nm. The frame rate of imaging is ~16 kHz, at A-line rate of ~1.62 MHz, which allows the detection of transient pulse waves with adequate temporal resolution. Imaging volumes with repeated B-scans are obtained on mouse retina capillary bed, and the mouse oxymeter signal is recorded simultaneously. The pulse wave on artery and vein are resolved, and with the synchronized heart beat signal, the temporal delay on different vessel locations is determined. The vessel specific measurement of PWV is achieved for the first time with SS-OCT, for pulse waves propagating more than 100 cm/s. Using the novel methodology of retinal PWV assessment, it is hoped that the clinical OCT scans can provide extended diagnostic information of cardiology functionalities.

Choroidal Thickness in 3001 Chinese Children Aged 6 to 19 Years Using Swept-Source OCT

PubMed Central

Xiong, Shuyu; He, Xiangui; Deng, Junjie; Lv, Minzhi; Jin, Jiali; Sun, Sifei; Yao, Chunxia; Zhu, Jianfeng; Zou, Haidong; Xu, Xun

2017-01-01

The purpose of the cross-sectional study is to describe the values and distribution of choroidal thickness and to explore its related factors, especially age, in Chinese children. A total of 3001 Chinese school children aged 6 to 19 years underwent comprehensive ophthalmic examinations, including axial length and cycloplegic refraction. Choroidal thickness was measured by swept-source optical coherence tomography (SS-OCT). There was a greater difference in the more central regions between the myopes and emmetropes. Multiple regression analysis was performed to determine the associated factors of choroidal thickness. The results demonstrated that age was independently positively related to choroidal thickness for emmetropes (β = 3.859, p < 0.001), and mild myopes with spherical equivalent greater than −2.00 D (−1.25 D < spherical equivalent ≤ −0.50 D: β = 3.476, p = 0.006; −2.00 D < spherical equivalent ≤ −1.25 D: β = 3.232, p = 0.020). However, no significant relationship between age and choroidal thickness was found in children with spherical equivalent ≤ −2.00 D, suggesting that the protective effect of physiologic choroidal growth with age against rapid axial elongation disappeared while axial elongation becomes the dominant determinant of choroidal thickness among children with myopia worse than −2.00 D. PMID:28327553

Diagnostic ability of macular ganglion cell inner plexiform layer measurements in glaucoma using swept source and spectral domain optical coherence tomography.

PubMed

Yang, Zhiyong; Tatham, Andrew J; Weinreb, Robert N; Medeiros, Felipe A; Liu, Ting; Zangwill, Linda M

2015-01-01

To evaluate the diagnostic ability of macular ganglion cell and inner plexiform layer measurements in glaucoma, obtained using swept source (SS) and spectral domain (SD) optical coherence tomography (OCT) and to compare to circumpapillary retinal nerve fiber layer (cpRNFL) thickness measurements. The study included 106 glaucomatous eyes of 80 subjects and 41 eyes of 22 healthy subjects from the Diagnostic Innovations in Glaucoma Study. Macular ganglion cell and inner plexiform layer (mGCIPL), macular ganglion cell complex (mGCC) and cpRNFL thickness were assessed using SS-OCT and SD-OCT, and area under the receiver operating characteristic curves (AUCs) were calculated to determine ability to differentiate glaucomatous and healthy eyes and between early glaucomatous and healthy eyes. Mean (± standard deviation) mGCIPL and mGCC thickness were thinner in both healthy and glaucomatous eyes using SS-OCT compared to using SD-OCT. Fixed and proportional biases were detected between SS-OCT and SD-OCT measures. Diagnostic accuracy (AUCs) for differentiating between healthy and glaucomatous eyes for average and sectoral mGCIPL was similar in SS-OCT (0.65 to 0.81) and SD-OCT (0.63 to 0.83). AUCs for average cpRNFL acquired using SS-OCT and SD-OCT tended to be higher (0.83 and 0.85, respectively) than for average mGCC (0.82 and 0.78, respectively), and mGCIPL (0.73 and 0.75, respectively) but these differences did not consistently reach statistical significance. Minimum SD-OCT mGCIPL and mGCC thickness (unavailable in SS-OCT) had the highest AUC (0.86) among macular measurements. Assessment of mGCIPL thickness using SS-OCT or SD-OCT is useful for detecting glaucomatous damage, but measurements are not interchangeable for patient management decisions. Diagnostic accuracies of mGCIPL and mGCC from both SS-OCT and SD-OCT were similar to that of cpRNFL for glaucoma detection.

Diagnostic Ability of Macular Ganglion Cell Inner Plexiform Layer Measurements in Glaucoma Using Swept Source and Spectral Domain Optical Coherence Tomography

PubMed Central

Yang, Zhiyong; Tatham, Andrew J.; Weinreb, Robert N.; Medeiros, Felipe A.; Liu, Ting; Zangwill, Linda M.

2015-01-01

Purpose To evaluate the diagnostic ability of macular ganglion cell and inner plexiform layer measurements in glaucoma, obtained using swept source (SS) and spectral domain (SD) optical coherence tomography (OCT) and to compare to circumpapillary retinal nerve fiber layer (cpRNFL) thickness measurements. Methods The study included 106 glaucomatous eyes of 80 subjects and 41 eyes of 22 healthy subjects from the Diagnostic Innovations in Glaucoma Study. Macular ganglion cell and inner plexiform layer (mGCIPL), macular ganglion cell complex (mGCC) and cpRNFL thickness were assessed using SS-OCT and SD-OCT, and area under the receiver operating characteristic curves (AUCs) were calculated to determine ability to differentiate glaucomatous and healthy eyes and between early glaucomatous and healthy eyes. Results Mean (± standard deviation) mGCIPL and mGCC thickness were thinner in both healthy and glaucomatous eyes using SS-OCT compared to using SD-OCT. Fixed and proportional biases were detected between SS-OCT and SD-OCT measures. Diagnostic accuracy (AUCs) for differentiating between healthy and glaucomatous eyes for average and sectoral mGCIPL was similar in SS-OCT (0.65 to 0.81) and SD-OCT (0.63 to 0.83). AUCs for average cpRNFL acquired using SS-OCT and SD-OCT tended to be higher (0.83 and 0.85, respectively) than for average mGCC (0.82 and 0.78, respectively), and mGCIPL (0.73 and 0.75, respectively) but these differences did not consistently reach statistical significance. Minimum SD-OCT mGCIPL and mGCC thickness (unavailable in SS-OCT) had the highest AUC (0.86) among macular measurements. Conclusion Assessment of mGCIPL thickness using SS-OCT or SD-OCT is useful for detecting glaucomatous damage, but measurements are not interchangeable for patient management decisions. Diagnostic accuracies of mGCIPL and mGCC from both SS-OCT and SD-OCT were similar to that of cpRNFL for glaucoma detection. PMID:25978420

Accuracy of Corneal Thickness by Swept-Source Optical Coherence Tomography and Scheimpflug Camera in Virgin and Treated Fuchs Endothelial Dystrophy.

PubMed

Arnalich-Montiel, Francisco; Ortiz-Toquero, Sara; Auladell, Clara; Couceiro, Ana

2018-06-01

To assess intraobserver repeatability, intersession reproducibility, and agreement of swept-source Fourier-domain optical coherence tomography (SS-OCT) and the Scheimpflug camera in measuring corneal thickness in virgin and grafted eyes with Fuchs endothelial corneal dystrophy (FECD). Thirty-six control eyes, 35 FECD eyes, 30 FECD with corneal edema eyes, 25 Descemet stripping automated endothelial keratoplasty (DSAEK) eyes, and 29 Descemet membrane endothelial keratoplasty (DMEK) eyes were included. The apical center, pupillary center, and thinnest corneal thickness were determined in 3 consecutive images and repeated 2 weeks later. Repeatability and reproducibility coefficients, intraclass correlation coefficients, and 95% limits of agreement (LOA) between measurements were calculated. Agreement between devices was assessed using Bland-Altman analysis. Corneal thickness measurements were highly reproducible and repeatable with both systems. SS-OCT showed better repeatability in all corneal locations in the normal, FECD, FECD with edema, DSAEK, and DMEK groups (coefficient of variation ≤0.60%, ≤0.36%, ≤0.43%, ≤1.09%, and ≤0.48%, respectively) than the Scheimpflug (coefficient of variation ≤1.15%, ≤0.92%, ≤1.10%, ≤1.25%, and ≤1.14%, respectively). Between-session 95% LOA for SS-OCT was less than 3% for all groups except for the FECD with edema group, being almost double using the Scheimpflug camera. Differences between instruments were statistically significant in all groups and locations (P < 0.01) except in the DSAEK group (P ≤ 0.51); however, SS-OCT underestimated all measurements. SS-OCT provides better reproducible and repeatable measurements of corneal thickness than those obtained with the Scheimpflug camera in patients with FECD or an endothelial transplant. Variations between examinations higher than the 95% LOA observed in our study should raise awareness of changes in the endothelial function.

Repeatability, interocular correlation and agreement of quantitative swept-source optical coherence tomography angiography macular metrics in healthy subjects.

PubMed

Fang, Danqi; Tang, Fang Yao; Huang, Haifan; Cheung, Carol Y; Chen, Haoyu

2018-05-29

To investigate the repeatability, interocular correlation and agreement of quantitative swept-source optical coherence tomography angiography (SS-OCTA) metrics in healthy subjects. Thirty-three healthy normal subjects were enrolled. The macula was scanned four times by an SS-OCTA system using the 3 mm×3 mm mode. The superficial capillary map images were analysed using a MATLAB program. A series of parameters were measured: foveal avascular zone (FAZ) area, FAZ perimeter, FAZ circularity, parafoveal vessel density, fractal dimension and vessel diameter index (VDI). The repeatability of four scans was determined by intraclass correlation coefficient (ICC). Then the averaged results were analysed for intereye difference, correlation and agreement using paired t-test, Pearson's correlation coefficient (r), ICC and Bland-Altman plot. The repeatability assessment of the macular metrics exported high ICC values (ranged from 0.853 to 0.996). There is no statistically significant difference in the OCTA metrics between the two eyes. FAZ area (ICC=0.961, r=0.929) and FAZ perimeter (ICC=0.884, r=0.802) showed excellent binocular correlation. Fractal dimension (ICC=0.732, r=0.578) and VDI (ICC=0.707, r=0.547) showed moderate binocular correlation, while parafoveal vessel density had poor binocular correlation. Bland-Altman plots showed the range of agreement was from -0.0763 to 0.0954 mm 2 for FAZ area and from -0.0491 to 0.1136 for parafoveal vessel density. The macular metrics obtained using SS-OCTA showed excellent repeatability in healthy subjects. We showed high intereye correlation in FAZ area and perimeter, moderate correlation in fractal dimension and VDI, while vessel density had poor correlation in normal healthy subjects. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

Gabor fusion master slave optical coherence tomography

PubMed Central

Cernat, Ramona; Bradu, Adrian; Israelsen, Niels Møller; Bang, Ole; Rivet, Sylvain; Keane, Pearse A.; Heath, David-Garway; Rajendram, Ranjan; Podoleanu, Adrian

2017-01-01

This paper describes the application of the Gabor filtering protocol to a Master/Slave (MS) swept source optical coherence tomography (SS)-OCT system at 1300 nm. The MS-OCT system delivers information from selected depths, a property that allows operation similar to that of a time domain OCT system, where dynamic focusing is possible. The Gabor filtering processing following collection of multiple data from different focus positions is different from that utilized by a conventional swept source OCT system using a Fast Fourier transform (FFT) to produce an A-scan. Instead of selecting the bright parts of A-scans for each focus position, to be placed in a final B-scan image (or in a final volume), and discarding the rest, the MS principle can be employed to advantageously deliver signal from the depths within each focus range only. The MS procedure is illustrated on creating volumes of data of constant transversal resolution from a cucumber and from an insect by repeating data acquisition for 4 different focus positions. In addition, advantage is taken from the tolerance to dispersion of the MS principle that allows automatic compensation for dispersion created by layers above the object of interest. By combining the two techniques, Gabor filtering and Master/Slave, a powerful imaging instrument is demonstrated. The master/slave technique allows simultaneous display of three categories of images in one frame: multiple depth en-face OCT images, two cross-sectional OCT images and a confocal like image obtained by averaging the en-face ones. We also demonstrate the superiority of MS-OCT over its FFT based counterpart when used with a Gabor filtering OCT instrument in terms of the speed of assembling the fused volume. For our case, we show that when more than 4 focus positions are required to produce the final volume, MS is faster than the conventional FFT based procedure. PMID:28270987

Macular retinal and choroidal thickness in unilateral amblyopia using swept-source optical coherence tomography.

PubMed

Araki, Syunsuke; Miki, Atsushi; Goto, Katsutoshi; Yamashita, Tsutomu; Takizawa, Go; Haruishi, Kazuko; Ieki, Yoshiaki; Kiryu, Junichi; Yaoeda, Kiyoshi

2017-09-15

To investigate macular retinal and choroidal thickness in amblyopic eyes compared to that in fellow and normal eyes using swept-source optical coherence tomography (SS-OCT). This study examined 31 patients with hyperopic anisometropic amblyopia (6.9 ± 3.8 years, mean ± standard deviation), 15 patients with strabismic amblyopia without anisometropia (7.9 ± 4.2 years), and 24 age-matched controls (7.8 ± 3.3 years). Retinal and choroidal thickness was measured by 3D scans using SS-OCT. A 6-mm area around the fovea was automatically analyzed using the Early Treatment Diabetic Retinopathy Study map. The thickness from SS-OCT was corrected for magnification error using individual axial length, spherical refraction, cylinder refraction, and corneal radius. Retinal thickness was divided into the macular retinal nerve fiber layer (mRNFL), ganglion cell layer + inner plexiform layer (GCL+IPL), ganglion cell complex (GCC), and the inner limiting membrane to the retinal pigment epithelium (ILM-RPE) thickness. Retinal and choroidal thickness was compared among amblyopic, fellow, and normal eyes. In both amblyopia groups, there was no significant difference in the mRNFL, GCL+IPL, and GCC thicknesses among the amblyopic, fellow, and control eyes. In the anisometropic amblyopia group, choroidal thickness (subfovea, center 1 mm, nasal and inferior of the inner ring, nasal of the outer ring, and center 6 mm) of amblyopic eyes were significantly greater than that of fellow and normal eyes. In contrast, none of the choroidal thicknesses were significantly different among the investigated eyes in the strabismic amblyopia group. We found no significant difference in inner retinal thickness in patients with unilateral amblyopia. Although there were significant differences in choroidal thickness with hyperopic anisometropic amblyopia, there was no significant difference for the strabismic amblyopia. The discrepancy in choroidal thickness between the two types of amblyopia may be due to both differences in ocular size and underlying mechanism.

En face choroidal vascular feature imaging in acute and chronic central serous chorioretinopathy using swept source optical coherence tomography.

PubMed

Lee, Won June; Lee, Jung Wook; Park, Seung Hun; Lee, Byung Ro

2017-05-01

To evaluate the variable depth tomographic features of choroidal vasculature in acute and chronic central serous chorioretinopathy (CSC) using swept source optical coherence tomography (SS-OCT) en face imaging. We retrospectively reviewed the en face SS-OCT images of 29 patients that presented with acute (12 eyes) or chronic (17 eyes) CSC. All of the patient eyes underwent 6×6 macular scans with SS-OCT (DRI OCT-1, Topcon, Tokyo, Japan), fluorescein angiography and indocyanine green angiography. The en face image was used to investigate the choroidal vasculature of each layer. Moreover, we determined that some parts corresponded to choriocapillaris and Sattler's layer attenuation, whereas choroidal vessel dilatation was associated with Haller's layer. At Haller's layer level, choroidal vessel dilatation was observed in 11 of 12 acute CSC (91.7%) and 15 of 17 chronic CSC (88.2%). In acute CSC, choroidal vessel dilatation was divided into focal (9/11; 81.8%) and diffuse (2/11; 18.2%) patterns. The chronic CSC cases demonstrated different patterns of choroidal vessel dilatation: focal (5/15; 33.3%) and diffuse (10/15; 66.6%). Ten of the acute CSC eyes (83.3%) and 14 of the chronic CSC eyes (82.4%) were found to have obscured choriocapillaris and Sattler's layers on en face imaging. En face imaging of SS-OCT is useful when combined with angiography in CSC for evaluating choroidal vessel dilatation at Haller's layer and to identify obscured upper layers. We identified different choroidal vessel dilatation patterns between acute and chronic CSC. These findings might be useful for pathophysiological understanding of CSC. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

The morphological changes of optically cleared cochlea using optical coherence tomography (Conference Presentation)

NASA Astrophysics Data System (ADS)

Lee, Jaeyul; Song, Jaewon; Jeon, Mansik; Kim, Jeehyun

2017-02-01

In this study, we monitored the optical clearing effects by immersing ex vivo guinea pig cochlea samples in ethylenediaminetetraacetic acid (EDTA) to study the internal microstructures in the morphology of guinea pig cochlea. The imaging limitations due to the guinea pig cochlea structures were overcome by optical clearing technique. Subsequently, the study was carried out to confirm the required approximate immersing duration of cochlea in EDTA-based optical clearing to obtain the best optimal depth visibility for guinea pig cochlea samples. Thus, we implemented a decalcification-based optical clearing effect to guinea pig cochlea samples to enhance the depth visualization of internal microstructures using swept source optical coherence tomography (OCT). The obtained nondestructive two-dimensional OCT images successfully illustrated the feasibility of the proposed method by providing clearly visible microstructures in the depth direction as a result of decalcification. The most optimal clearing outcomes for the guinea pig cochlea were obtained after 14 consecutive days. The quantitative assessment results verified the increase of the intensity as well as the thickness measurements of the internal microstructures. Following this method, difficulties in imaging of internal cochlea microstructures of guinea pigs could be avoided. The obtained results verified that the depth visibility of the decalcified ex vivo guinea pig cochlea samples was enhanced. Therefore, the proposed EDTA-based optical clearing method for guinea pig can be considered as a potential application for depth-enhanced OCT visualization.

SNR of swept SLEDs and swept lasers for OCT (Conference Presentation)

NASA Astrophysics Data System (ADS)

Johnson, Bart C.; Atia, Walid; Flanders, Dale; Kuznetsov, Mark; Goldberg, Brian; Kemp, Nate; Whitney, Peter

2016-03-01

A back-to-back comparison of a tunable narrow-band SLED (TSLED) and a swept laser are made for OCT applications. Both are 1310 nm sources sweeping at 50 kHz over a 100 nm tuning range and have similar coherence lengths. The TSLED consists of a seed SOA and two amplification SOAs. The ASE is filtered twice by a tunable MEMS Fabry Perot in a polarization multiplexed double-pass arrangement on either side of the middle SOA. This allows very long coherence lengths to be achieved. A fundamental issue with a SLED is that the RIN is proportional to 1/Linewidth, meaning that the longer the coherence length, the higher the RIN. High RIN also leads to increased clock jitter. Most swept source SNR calculations assume that the noise is independent of the amplitude of the signal light: The higher the signal, the higher the SNR. We show that in the case of the TSLED, that the high signal RIN and clock jitter give rise to additional noises that scale with signal power. This leads to an SNR limit in the case of the TSLED: The higher the signal, the higher the noise, so the SNR reaches a limit. While the TSLED has respectable sensitivity, the SNR limit causes noise streaks in an image where the A-line has a high reflectivity point. The laser, which is shot noise limited, does not exhibit this effect. This is illustrated with SNR data and side-by-side images taken with the two sources.

High peak-power laser system tuneable from 8 to 10 μm

NASA Astrophysics Data System (ADS)

Gutty, François; Grisard, Arnaud; Larat, Christian; Papillon, Dominique; Schwarz, Muriel; Gérard, Bruno; Ostendorf, Ralf; Wagner, Joachim; Lallier, Eric

2017-04-01

A high peak-power rapidly tuneable laser system in the long-wave infrared is obtained using an external cavity quantum-cascade laser (EC-QCL) broadly tuneable from 8 to 10 μm and an optical parametric amplifier (OPA) based on quasi phase-matching in orientation-patterned gallium arsenide (OP-GaAs). To provide an efficient amplification, the nonlinear crystal is pumped by a pulsed fiber laser source. With a pump laser source tuneable around 2 μm, quasi phase-matching remains satisfied with a fixed grating period in the OP-GaAs crystal when the EC-QCL wavelength is swept from 8 to 10 μm. The OPA demonstrates parametric amplification from 8 to 10 μm and achieves output peak powers up to 140 W, with spectral linewidths below 3.5 cm-1 and a beam profile quality (M2) below 3.4 in both horizontal and vertical directions.

In vivo imaging of human oral hard and soft tissues by polarization-sensitive optical coherence tomography

NASA Astrophysics Data System (ADS)

Walther, Julia; Golde, Jonas; Kirsten, Lars; Tetschke, Florian; Hempel, Franz; Rosenauer, Tobias; Hannig, Christian; Koch, Edmund

2017-12-01

Since optical coherence tomography (OCT) provides three-dimensional high-resolution images of biological tissue, the benefit of polarization contrast in the field of dentistry is highlighted in this study. Polarization-sensitive OCT (PS OCT) with phase-sensitive recording is used for imaging dental and mucosal tissues in the human oral cavity in vivo. An enhanced polarization contrast of oral structures is reached by analyzing the signals of the co- and crosspolarized channels of the swept source PS OCT system quantitatively with respect to reflectivity, retardation, optic axis orientation, and depolarization. The calculation of these polarization parameters enables a high tissue-specific contrast imaging for the detailed physical interpretation of human oral hard and soft tissues. For the proof-of-principle, imaging of composite restorations and mineralization defects at premolars as well as gingival, lingual, and labial oral mucosa was performed in vivo within the anterior oral cavity. The achieved contrast-enhanced results of the investigated human oral tissues by means of polarization-sensitive imaging are evaluated by the comparison with conventional intensity-based OCT.

Real time en face Fourier-domain optical coherence tomography with direct hardware frequency demodulation

PubMed Central

Biedermann, Benjamin R.; Wieser, Wolfgang; Eigenwillig, Christoph M.; Palte, Gesa; Adler, Desmond C.; Srinivasan, Vivek J.; Fujimoto, James G.; Huber, Robert

2009-01-01

We demonstrate en face swept source optical coherence tomography (ss-OCT) without requiring a Fourier transformation step. The electronic optical coherence tomography (OCT) interference signal from a k-space linear Fourier domain mode-locked laser is mixed with an adjustable local oscillator, yielding the analytic reflectance signal from one image depth for each frequency sweep of the laser. Furthermore, a method for arbitrarily shaping the spectral intensity profile of the laser is presented, without requiring the step of numerical apodization. In combination, these two techniques enable sampling of the in-phase and quadrature signal with a slow analog-to-digital converter and allow for real-time display of en face projections even for highest axial scan rates. Image data generated with this technique is compared to en face images extracted from a three-dimensional OCT data set. This technique can allow for real-time visualization of arbitrarily oriented en face planes for the purpose of alignment, registration, or operator-guided survey scans while simultaneously maintaining the full capability of high-speed volumetric ss-OCT functionality. PMID:18978919

Direct composite fillings: an optical coherence tomography and microCT investigation

NASA Astrophysics Data System (ADS)

Negrutiu, Meda L.; Sinescu, Cosmin; Borlea, Mugurel V.; Manescu, Adrian; Duma, Virgil F.; Rominu, Mihai; Podoleanu, Adrian G.

2015-03-01

The treatment of carious lesions requires removal of affected dental tissue thus creating cavities that are to be filled with dedicated materials. There are several methods known which are used to assess the quality of direct dental restorations, but most of them are invasive. Optical tomographic techniques are of particular importance in the medical imaging field, because these techniques can provide non-invasive diagnostic images. Using an en-face version of OCT, we have recently demonstrated real time thorough evaluation of quality of dental fillings. The major aim of this study was to analyses the optical performance of adhesives modified with zirconia particles in different concentrations in order to improve the contrast of OCT imaging of the interface between the tooth structure, adhesive and composite resin. The OCT investigations were validated by micro CT using synchrotron radiation. The OCT Swept Source is a valuable investigation tool for the clinical evaluation of class II direct composite restorations. The unmodified adhesive layer shows poor contrast on regular OCT investigations. Adding zirconia particles to the adhesive layer provides a better scattering which allows a better characterization and quantification of direct restorations.

Tortuous Pore Path Through the Glaucomatous Lamina Cribrosa.

PubMed

Wang, Bo; Lucy, Katie A; Schuman, Joel S; Sigal, Ian A; Bilonick, Richard A; Lu, Chen; Liu, Jonathan; Grulkowski, Ireneusz; Nadler, Zachary; Ishikawa, Hiroshi; Kagemann, Larry; Fujimoto, James G; Wollstein, Gadi

2018-05-08

The lamina cribrosa is a primary site of damage in glaucoma. While mechanical distortion is hypothesized to cause reduction of axoplasmic flow, little is known about how the pores, which contains the retinal ganglion cell axons, traverse the lamina cribrosa. We investigated lamina cribrosa pore paths in vivo to quantify differences in tortuosity of pore paths between healthy and glaucomatous eyes. We imaged 16 healthy, 23 glaucoma suspect and 48 glaucomatous eyes from 70 subjects using a swept source optical coherence tomography system. The lamina cribrosa pores were automatically segmented using a previously described segmentation algorithm. Individual pore paths were automatically tracked through the depth of the lamina cribrosa using custom software. Pore path convergence to the optic nerve center and tortuosity was quantified for each eye. We found that lamina cribrosa pore pathways traverse the lamina cribrosa closer to the optic nerve center along the depth of the lamina cribrosa regardless of disease severity or diagnostic category. In addition, pores of glaucoma eyes take a more tortuous path through the lamina cribrosa compared to those of healthy eyes, suggesting a potential mechanism for reduction of axoplasmic flow in glaucoma.

Robust and fast characterization of OCT-based optical attenuation using a novel frequency-domain algorithm for brain cancer detection

NASA Astrophysics Data System (ADS)

Yuan, Wu; Kut, Carmen; Liang, Wenxuan; Li, Xingde

2017-03-01

Cancer is known to alter the local optical properties of tissues. The detection of OCT-based optical attenuation provides a quantitative method to efficiently differentiate cancer from non-cancer tissues. In particular, the intraoperative use of quantitative OCT is able to provide a direct visual guidance in real time for accurate identification of cancer tissues, especially these without any obvious structural layers, such as brain cancer. However, current methods are suboptimal in providing high-speed and accurate OCT attenuation mapping for intraoperative brain cancer detection. In this paper, we report a novel frequency-domain (FD) algorithm to enable robust and fast characterization of optical attenuation as derived from OCT intensity images. The performance of this FD algorithm was compared with traditional fitting methods by analyzing datasets containing images from freshly resected human brain cancer and from a silica phantom acquired by a 1310 nm swept-source OCT (SS-OCT) system. With graphics processing unit (GPU)-based CUDA C/C++ implementation, this new attenuation mapping algorithm can offer robust and accurate quantitative interpretation of OCT images in real time during brain surgery.

Swept Line Electron Beam Annealing of Ion Implanted Semiconductors.

DTIC Science & Technology

1982-07-01

of my research to the mainstream of technology. The techniques used for beam processing are distinguished by their * ~.* beam source and method by...raster scanned CW lasers (CWL), pulsed ion beams (PI), area pulsed electron beams (PEE), raster scanned (RSEB) or multi - scanned electron beams (MSEB...where high quality or tailored profiles are required. Continuous wave lasers and multi -scanned or swept-line electron beams are the most likely candidates

Combined two-photon microscopy and optical coherence tomography using individually optimized sources

NASA Astrophysics Data System (ADS)

Jeong, Bosu; Lee, Byunghak; Jang, Min Seong; Nam, Hyoseok; Kim, Hae Koo; Yoon, Sang June; Doh, Junsang; Lee, Sang-Joon; Yang, Bo-Gie; Jang, Myoung Ho; Kim, Ki Hean

2011-03-01

Two-photon microscopy (TPM) and optical coherence tomography (OCT) are 3D tissue imaging techniques based on different contrast mechanisms. We developed a combined system of TPM and OCT to provide information of both imaging modalities for in-vivo tissue study. TPM and OCT were implemented by using separate light sources, a Ti-Sapphire laser and a wavelength-swept source centered at 1300 nm respectively, and scanners. Light from the two sources was combined for the simultaneous imaging of tissue samples. TPM provided molecular, cellular information of tissues in the region of a few hundred microns on one side at a sub-cellular resolution, and ran at approximately 40 frames per second. OCT provided structural information in the tissue region larger than TPM images at a sub-tenth micron resolution by using 0.1 numerical aperture. OCT had the field of view of 800 um × 800 um based on a 20x objective, the sensitivity of 97dB, and the imaging speed of 0.8 volumes per second. This combined system was tested with simple microsphere specimens, and then was applied to image the explanted intestine of a mouse model and the plant leaves. Morphology and micro-structures of the intestine villi and immune cells within the villi were shown in the intestine image, and chloroplasts and various microstructures of the maize leaves were visualized in 3D by the combined system.

Optical clearing of melanoma in vivo: characterization by diffuse reflectance spectroscopy and optical coherence tomography

NASA Astrophysics Data System (ADS)

Pires, Layla; Demidov, Valentin; Vitkin, I. Alex; Bagnato, Vanderlei; Kurachi, Cristina; Wilson, Brian C.

2016-08-01

Melanoma is the most aggressive type of skin cancer, with significant risk of fatality. Due to its pigmentation, light-based imaging and treatment techniques are limited to near the tumor surface, which is inadequate, for example, to evaluate the microvascular density that is associated with prognosis. White-light diffuse reflectance spectroscopy (DRS) and near-infrared optical coherence tomography (OCT) were used to evaluate the effect of a topically applied optical clearing agent (OCA) in melanoma in vivo and to image the microvascular network. DRS was performed using a contact fiber optic probe in the range from 450 to 650 nm. OCT imaging was performed using a swept-source system at 1310 nm. The OCT image data were processed using speckle variance and depth-encoded algorithms. Diffuse reflectance signals decreased with clearing, dropping by ˜90% after 45 min. OCT was able to image the microvasculature in the pigmented melanoma tissue with good spatial resolution up to a depth of ˜300 μm without the use of OCA; improved contrast resolution was achieved with optical clearing to a depth of ˜750 μm in tumor. These findings are relevant to potential clinical applications in melanoma, such as assessing prognosis and treatment responses. Optical clearing may also facilitate the use of light-based treatments such as photodynamic therapy.

Injection-depth-locking axial motion guided handheld micro-injector using CP-SSOCT.

PubMed

Cheon, Gyeong Woo; Huang, Yong; Kwag, Hye Rin; Kim, Ki-Young; Taylor, Russell H; Gehlbach, Peter L; Kang, Jin U

2014-01-01

This paper presents a handheld micro-injector system using common-path swept source optical coherence tomography (CP-SSOCT) as a distal sensor with highly accurate injection-depth-locking. To achieve real-time, highly precise, and intuitive freehand control, the system used graphics processing unit (GPU) to process the oversampled OCT signal with high throughput and a smart customized motion monitoring control algorithm. A performance evaluation was conducted with 60-insertions and fluorescein dye injection tests to show how accurately the system can guide the needle and lock to the target depth. The evaluation tests show our system can guide the injection needle into the desired depth with 4.12 um average deviation error while injecting 50 nl of fluorescein dye.

Comparative study of shear wave-based elastography techniques in optical coherence tomography

NASA Astrophysics Data System (ADS)

Zvietcovich, Fernando; Rolland, Jannick P.; Yao, Jianing; Meemon, Panomsak; Parker, Kevin J.

2017-03-01

We compare five optical coherence elastography techniques able to estimate the shear speed of waves generated by one and two sources of excitation. The first two techniques make use of one piezoelectric actuator in order to produce a continuous shear wave propagation or a tone-burst propagation (TBP) of 400 Hz over a gelatin tissue-mimicking phantom. The remaining techniques utilize a second actuator located on the opposite side of the region of interest in order to create three types of interference patterns: crawling waves, swept crawling waves, and standing waves, depending on the selection of the frequency difference between the two actuators. We evaluated accuracy, contrast to noise ratio, resolution, and acquisition time for each technique during experiments. Numerical simulations were also performed in order to support the experimental findings. Results suggest that in the presence of strong internal reflections, single source methods are more accurate and less variable when compared to the two-actuator methods. In particular, TBP reports the best performance with an accuracy error <4.1%. Finally, the TBP was tested in a fresh chicken tibialis anterior muscle with a localized thermally ablated lesion in order to evaluate its performance in biological tissue.

Simultaneous multi-laser, multi-species trace-level sensing of gas mixtures by rapidly swept continuous-wave cavity-ringdown spectroscopy.

PubMed

He, Yabai; Kan, Ruifeng; Englich, Florian V; Liu, Wenqing; Orr, Brian J

2010-09-13

The greenhouse-gas molecules CO(2), CH(4), and H(2)O are detected in air within a few ms by a novel cavity-ringdown laser-absorption spectroscopy technique using a rapidly swept optical cavity and multi-wavelength coherent radiation from a set of pre-tuned near-infrared diode lasers. The performance of various types of tunable diode laser, on which this technique depends, is evaluated. Our instrument is both sensitive and compact, as needed for reliable environmental monitoring with high absolute accuracy to detect trace concentrations of greenhouse gases in outdoor air.

Wide-field in vivo oral OCT imaging

PubMed Central

Lee, Anthony M. D.; Cahill, Lucas; Liu, Kelly; MacAulay, Calum; Poh, Catherine; Lane, Pierre

2015-01-01

We have built a polarization-sensitive swept source Optical Coherence Tomography (OCT) instrument capable of wide-field in vivo imaging in the oral cavity. This instrument uses a hand-held side-looking fiber-optic rotary pullback catheter that can cover two dimensional tissue imaging fields approximately 2.5 mm wide by up to 90 mm length in a single image acquisition. The catheter spins at 100 Hz with pullback speeds up to 15 mm/s allowing imaging of areas up to 225 mm2 field-of-view in seconds. A catheter sheath and two optional catheter sheath holders have been designed to allow imaging at all locations within the oral cavity. Image quality of 2-dimensional image slices through the data can be greatly enhanced by averaging over the orthogonal dimension to reduce speckle. Initial in vivo imaging results reveal a wide-field view of features such as epithelial thickness and continuity of the basement membrane that may be useful in clinic for chair-side management of oral lesions. PMID:26203389

Monitoring tooth demineralization using a cross polarization optical coherence tomographic system with an integrated MEMS scanner

NASA Astrophysics Data System (ADS)

Fried, Daniel; Staninec, Michal; Darling, Cynthia; Kang, Hobin; Chan, Kenneth

2012-01-01

New methods are needed for the nondestructive measurement of tooth demineralization and remineralization to monitor the progression of incipient caries lesions (tooth decay) for effective nonsurgical intervention and to evaluate the performance of anti-caries treatments such as chemical treatments or laser irradiation. Studies have shown that optical coherence tomography (OCT) has great potential to fulfill this role since it can be used to measure the depth and severity of early lesions with an axial resolution exceeding 10-μm, it is easy to apply in vivo and it can be used to image the convoluted topography of tooth occlusal surfaces. In this paper we present early results using a new cross-polarization OCT system introduced by Santec. This system utilizes a swept laser source and a MEMS scanner for rapid acquisition of cross polarization images. Preliminary studies show that this system is useful for measurement of the severity of demineralization on tooth surfaces and for showing the spread of occlusal lesions under the dentinal-enamel junction.

Morphological analysis of the growth stages of in-vivo mouse hair follicles by using optical coherence tomography

NASA Astrophysics Data System (ADS)

Jha, Rakesh Kumar; Kim, Kanghae; Jeon, Mansik; Kim, Jeehyun; Kang, Minyoung; Han, Insook; Kim, Moonkyu

2016-09-01

Swept-source optical coherence tomography (SS-OCT), a bio-photonic imaging modality, was used to demonstrate an initial feasibility experiment for detecting morphological variations of in-vivo mouse hair follicles for the anagen and the telogen growth stages. Two C57BL/6 adult male mice, one undergoing the anagen stage and the other undergoing the telogen stage of the hair follicle growth cycle, were selected for the experiment. The OCT cross-sectional images of mice skin were acquired in-vivo within an interval of 15 days, and the observed morphological changes were analyzed. The micro-structural features of mice skin on the 15th experimental day were further compared with corresponding histological observations. The preliminary result of this study provides clear insights into the structural details of mouse skin, confirming the resemblance of the OCT images with the corresponding histological measurements, and ensures the suitability of SS-OCT for non-invasive analysis of hair follicle conditions.

Multimodal imaging of bilateral diffuse uveal melanocytic proliferation associated with an iris mass lesion.

PubMed

Naysan, Jonathan; Pang, Claudine E; Klein, Robert W; Freund, K Bailey

2016-01-01

Bilateral diffuse uveal melanocytic proliferation (BDUMP) is a rare, paraneoplastic syndrome characterized by bilateral painless visual loss and proliferation of choroidal melanocytes in association with an underlying systemic malignancy. We report a case of bilateral diffuse uveal melanocytic proliferation associated with an underlying gynecological malignancy that also features the infrequent finding of an iris mass lesion, using multimodal imaging including ultra-widefield imaging, spectral domain and swept-source optical coherence tomography. A 59-year-old white female with a prior history of gynecological malignancy in remission presented with progressive bilateral visual loss over several weeks. The patient was noted to have a focal iris mass lesion in her right eye. Ultra-widefield color fundus photography showed a characteristic bilateral 'giraffe pattern' of pigmentary changes extending into the periphery as well as multiple discrete deeply pigmented lesions. Ultra-widefield autofluorescence was useful for visualizing the full extent of involvement. Indocyanine green angiography helped to demarcate the discrete pigmented choroidal lesions. Swept-source OCT clearly delineated the alternating zones of retinal pigment epithelium (RPE) thickening and RPE loss, as well as the prominent choroidal infiltration and thickening. BDUMP is an important diagnosis to consider in the presence of multiple discrete melanocytic choroidal lesions, diffuse choroidal thickening, characteristic RPE changes, iris mass lesions and exudative retinal detachment. Ultra-widefield imaging may demonstrate more extensive lesions than that detected on clinical examination or standard field imaging. Imaging with SS-OCT shows choroidal and RPE characteristics that correlate well with known histopathology of this entity.

TOWARD QUANTITATIVE OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY: Visualizing Blood Flow Speeds in Ocular Pathology Using Variable Interscan Time Analysis.

PubMed

Ploner, Stefan B; Moult, Eric M; Choi, WooJhon; Waheed, Nadia K; Lee, ByungKun; Novais, Eduardo A; Cole, Emily D; Potsaid, Benjamin; Husvogt, Lennart; Schottenhamml, Julia; Maier, Andreas; Rosenfeld, Philip J; Duker, Jay S; Hornegger, Joachim; Fujimoto, James G

2016-12-01

Currently available optical coherence tomography angiography systems provide information about blood flux but only limited information about blood flow speed. The authors develop a method for mapping the previously proposed variable interscan time analysis (VISTA) algorithm into a color display that encodes relative blood flow speed. Optical coherence tomography angiography was performed with a 1,050 nm, 400 kHz A-scan rate, swept source optical coherence tomography system using a 5 repeated B-scan protocol. Variable interscan time analysis was used to compute the optical coherence tomography angiography signal from B-scan pairs having 1.5 millisecond and 3.0 milliseconds interscan times. The resulting VISTA data were then mapped to a color space for display. The authors evaluated the VISTA visualization algorithm in normal eyes (n = 2), nonproliferative diabetic retinopathy eyes (n = 6), proliferative diabetic retinopathy eyes (n = 3), geographic atrophy eyes (n = 4), and exudative age-related macular degeneration eyes (n = 2). All eyes showed blood flow speed variations, and all eyes with pathology showed abnormal blood flow speeds compared with controls. The authors developed a novel method for mapping VISTA into a color display, allowing visualization of relative blood flow speeds. The method was found useful, in a small case series, for visualizing blood flow speeds in a variety of ocular diseases and serves as a step toward quantitative optical coherence tomography angiography.

Evaluation of circumferential angle closure using iridotrabecular contact index after laser iridotomy by swept-source optical coherence tomography.

PubMed

Cho, Hyun-Kyung; Ahn, Dongsub; Kee, Changwon

2017-05-01

To investigate the quantitative changes of circumferential angle closure after laser iridotomy (LI) using the iridotrabecular contact (ITC) index by Swept-Source optical coherence tomography (OCT). In this prospective observational study conducted in a hospital setting, 42 eyes of 36 patients (five males, 31 females) who underwent LI were included. The mean age was 65.00 ± 8.13 years old and the diagnosis included primary angle closure (PAC, 21 eyes), PAC suspect (16 eyes) and PAC glaucoma (five eyes). Optical coherence tomography (OCT) images were obtained pre-LI and at 1 week post-LI. In each image frame, the scleral spur (SS) and the ITC end-point were marked, from which the ITC index was calculated as a percentage of the angle closure across 360°. Measurements inspected before and after LI included: central anterior chamber depth (ACD), anterior chamber volume (ACV), lens vault (LV), nasal and temporal angle opening distance (AOD), angle recess area (ARA), trabecular-iris space area (TISA), trabecular-iris angle (TIA) at 500 μm and 750 μm from the SS and intraocular pressure (IOP). The ITC index and IOP decreased significantly after LI from 71.52 ± 26.29 to 35.31 ± 27.19 and from 20.64 ± 12.72 mmHg to 14.02 ± 3.49 mmHg, respectively (p < 0.001 and p < 0.001). Central ACD (1.94 ± 0.31 mm pre-LI) and LV (1.13 ± 0.32 mm pre-LI) did not show a significant change after LI (all p > 0.05), but ACV increased significantly after LI (p < 0.001). Most of the angle parameters except for nasal TIAs increased significantly after LI (all p < 0.05). The ITC index from patients with shallow anterior chamber angle showed a significant decrease after LI, but part of the angle closure was not relieved after LI. Other mechanisms besides pupillary block may play a role together in causing angle closure. © 2016 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.

Femtosecond Optics: Advanced Devices and Ultrafast Phenomena

DTIC Science & Technology

2007-05-31

repetition rate from a soliton fiber laser [6]. Because the mode- locking mechanism is passive, no external oscillator is required, leading to a more...nonlinearity, 1.8 m of LNL-SMF is included in the laser. Mode- locked operation of the laser was obtained through nonlinear polarization evolution [6]. For pump...Generation in Photonic Crystal Fibers for Optical Coherence Tomography H. Frequency Swept Lasers and Fourier Domain Mode Locking (FDML) I. Physics of

Clinical optical coherence tomography combined with multiphoton tomography for evaluation of several skin disorders

NASA Astrophysics Data System (ADS)

König, Karsten; Speicher, Marco; Bückle, Rainer; Reckfort, Julia; McKenzie, Gordon; Welzel, Julia; Koehler, Martin J.; Elsner, Peter; Kaatz, Martin

2010-02-01

The first clinical trial of optical coherence tomography (OCT) combined with multiphoton tomography (MPT) and dermoscopy is reported. State-of-the-art (i) OCT systems for dermatology (e.g. multibeam swept source OCT), (ii) the femtosecond laser multiphoton tomograph DermaInspectTM, and (iii) digital dermoscopes were applied to 47 patients with a diversity of skin diseases and disorders such as skin cancer, psoriasis, hemangioma, connective tissue diseases, pigmented lesions, and autoimmune bullous skin diseases. Dermoscopy, also called 'epiluminescent microscopy', provides two-dimensional color images of the skin surface. OCT imaging is based on the detection of optical reflections within the tissue measured interferometrically whereas nonlinear excitation of endogenous fluorophores and the second harmonic generation are the bases of MPT images. OCT cross sectional "wide field" image provides a typical field of view of 5 x 2 mm2 and offers fast information on the depth and the volume of the investigated lesion. In comparison, multiphoton tomography presents 0.36 x 0.36 mm2 horizontal or diagonal sections of the region of interest within seconds with submicron resolution and down to a tissue depth of 200 μm. The combination of OCT and MPT provides a synergistic optical imaging modality for early detection of skin cancer and other skin diseases.

Miniature endoscopic optical coherence tomography for calculus detection.

PubMed

Kao, Meng-Chun; Lin, Chun-Li; Kung, Che-Yen; Huang, Yi-Fung; Kuo, Wen-Chuan

2015-08-20

The effective treatment of periodontitis involves the detection and removal of subgingival dental calculus. However, subgingival calculus is more difficult to detect than supragingival calculus because it is firmly attached to root surfaces within periodontal pockets. To achieve a smooth root surface, clinicians often remove excessive amounts of root structure because of decreased visibility. In addition, enamel pearl, a rare type of ectopic enamel formation on the root surface, can easily be confused with dental calculus in the subgingival environment. In this study, we developed a fiber-probe swept-source optical coherence tomography (SSOCT) technique and combined it with the quantitative measurement of an optical parameter [standard deviation (SD) of the optical coherence tomography (OCT) intensity] to differentiate subgingival calculus from sound enamel, including enamel pearl. Two-dimensional circumferential images were constructed by rotating the miniprobe (0.9 mm diameter) while acquiring image lines, and the adjacent lines in each rotation were stacked to generate a three-dimensional volume. In OCT images, compared to sound enamel and enamel pearls, dental calculus showed significant differences (P<0.001) in SD values. Finally, the receiver operating characteristic curve had a high capacity (area under the curve=0.934) for discriminating between healthy regions (including enamel pearl) and dental calculus.

Optical sensing method to analyze germination rate of Capsicum annum seeds treated with growth-promoting chemical compounds using optical coherence tomography

NASA Astrophysics Data System (ADS)

Wijesinghe, Ruchire Eranga; Lee, Seung-Yeol; Kim, Pilun; Jung, Hee-Young; Jeon, Mansik; Kim, Jeehyun

2017-09-01

Seed germination rate differs based on chemical treatments, and nondestructive measurements of germination rate have become an essential requirement in the field of agriculture. Seed scientists and other biologists are interested in optical sensing technologies-based biological discoveries due to nondestructive detection capability. Optical coherence tomography (OCT) has recently emerged as a powerful method for biological and plant material discoveries. We report an extended application of OCT by monitoring the germination rate acceleration of chemically primed seeds. To validate the versatility of the method, Capsicum annum seeds were primed using three chemical compounds: sterile distilled water (SDW), butandiol, and 1-hexadecene. Monitoring was performed using a 1310-nm swept source OCT system. The results confirmed more rapid morphological variations in the seeds treated with 1-hexadecene medium than the seeds treated with SDW and butandiol within 8 consecutive days. In addition, fresh weight measurements (gold standard) of seeds were monitored for 15 days, and the obtained results were correlated with the OCT results. Thus, such a method can be used in various agricultural fields, and OCT shows potential as a rigorous sensing method for selecting the optimal plant growth-promoting chemical compounds rapidly, when compared with the gold standard methods.

Clinical optical coherence tomography combined with multiphoton tomography of patients with skin diseases.

PubMed

König, Karsten; Speicher, Marco; Bückle, Rainer; Reckfort, Julia; McKenzie, Gordon; Welzel, Julia; Koehler, Martin J; Elsner, Peter; Kaatz, Martin

2009-07-01

We report on the first clinical study based on optical coherence tomography (OCT) in combination with multiphoton tomography (MPT) and dermoscopy. 47 patients with a variety of skin diseases and disorders such as skin cancer, psoriasis, hemangioma, connective tissue diseases, pigmented lesions, and autoimmune bullous skin diseases have been investigated with (i) state-of-the-art OCT systems for dermatology including multibeam swept source OCT, (ii) the femtosecond laser multiphoton tomograph, and (iii) dermoscopes. Dermoscopy provides two-dimensional color images of the skin surface. OCT images reflect modifications of the intratissue refractive index whereas MPT is based on nonlinear excitation of endogenous fluorophores and second harmonic generation. A stack of cross-sectional OCT "wide field" images with a typical field of view of 5 x 2 mm(2) gave fast information on the depth and the volume of the lesion. Multiphoton tomography provided 0.36 x 0.36 mm(2) horizontal/diagonal optical sections within seconds of a particular region of interest with superior submicron resolution down to a tissue depth of 200 mum. The combination of OCT and MPT provides a unique powerful optical imaging modality for early detection of skin cancer and other skin diseases as well as for the evaluation of the efficiency of treatments.

Fiber-based polarization-sensitive OCT of the human retina with correction of system polarization distortions

PubMed Central

Braaf, Boy; Vermeer, Koenraad A.; de Groot, Mattijs; Vienola, Kari V.; de Boer, Johannes F.

2014-01-01

In polarization-sensitive optical coherence tomography (PS-OCT) the use of single-mode fibers causes unpredictable polarization distortions which can result in increased noise levels and erroneous changes in calculated polarization parameters. In the current paper this problem is addressed by a new Jones matrix analysis method that measures and corrects system polarization distortions as a function of wavenumber by spectral analysis of the sample surface polarization state and deeper located birefringent tissue structures. This method was implemented on a passive-component depth-multiplexed swept-source PS-OCT system at 1040 nm which was theoretically modeled using Jones matrix calculus. High-resolution B-scan images are presented of the double-pass phase retardation, diattenuation, and relative optic axis orientation to show the benefits of the new analysis method for in vivo imaging of the human retina. The correction of system polarization distortions yielded reduced phase retardation noise, and better estimates of the diattenuation and the relative optic axis orientation in weakly birefringent tissues. The clinical potential of the system is shown by en face visualization of the phase retardation and optic axis orientation of the retinal nerve fiber layer in a healthy volunteer and a glaucoma patient with nerve fiber loss. PMID:25136498

In vivo imaging of microvascular changes in inflammatory human skin induced by tape stripping and mosquito saliva using optical microangiography

NASA Astrophysics Data System (ADS)

Baran, Utku; Choi, Woo J.; Wang, Ruikang K.

2015-03-01

Tape stripping on human skin induces mechanical disruptions of the epidermal barrier that lead to minor skin inflammation which leads to temporary changes in microvasculature. On the other hand, when mosquitoes probe the skin for blood feeding, they inject saliva in dermal tissue. Mosquito saliva is known to exert various biological activities, such as dermal mast cell degranulation, leading to fluid extravasation and neutrophil influx. This inflammatory response remain longer than the tape stripping caused inflammation. In this study, we demonstrate the capabilities of swept-source optical coherence tomography (OCT) in detecting in vivo microvascular response of inflammatory human skin. Optical microangiography (OMAG), noninvasive volumetric microvasculature in vivo imaging method, has been used to track the vascular responses after tape stripping and mosquito bite. Vessel density has been quantified and used to correlate with the degree of skin irritation. The proved capability of OMAG technique in visualizing the microvasculature network under inflamed skin condition can play an important role in clinical trials of treatment and diagnosis of inflammatory skin disorders as well as studying mosquito bite's perception by the immune system and its role in parasite transmission.

Quasi-supercontinuum source in the extreme ultraviolet using multiple frequency combs from high-harmonic generation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wünsche, Martin; Fuchs, Silvio; Aull, Stefan

A quasi-supercontinuum source in the extreme ultraviolet (XUV) is demonstrated using a table-top femtosecond laser and a tunable optical parametric amplifier (OPA) as a driver for high-harmonic generation (HHG). The harmonic radiation, which is usually a comb of odd multiples of the fundamental frequency, is generated by near-infrared (NIR) laser pulses from the OPA. A quasi-continuous XUV spectrum in the range of 30 to 100 eV is realized by averaging over multiple harmonic comb spectra with slightly different fundamental frequencies and thus different spectral spacing between the individual harmonics. The driving laser wavelength is swept automatically during an averaging timemore » period. With a total photon flux of 4×10 9 photons/s in the range of 30 eV to 100 eV and 1×10 7 photons/s in the range of 100 eV to 200 eV, the resulting quasi-supercontinuum XUV source is suited for applications such as XUV coherence tomography (XCT) or near-edge absorption fine structure spectroscopy (NEXAFS).« less

Quasi-supercontinuum source in the extreme ultraviolet using multiple frequency combs from high-harmonic generation

DOE PAGES

Wünsche, Martin; Fuchs, Silvio; Aull, Stefan; ...

2017-03-16

A quasi-supercontinuum source in the extreme ultraviolet (XUV) is demonstrated using a table-top femtosecond laser and a tunable optical parametric amplifier (OPA) as a driver for high-harmonic generation (HHG). The harmonic radiation, which is usually a comb of odd multiples of the fundamental frequency, is generated by near-infrared (NIR) laser pulses from the OPA. A quasi-continuous XUV spectrum in the range of 30 to 100 eV is realized by averaging over multiple harmonic comb spectra with slightly different fundamental frequencies and thus different spectral spacing between the individual harmonics. The driving laser wavelength is swept automatically during an averaging timemore » period. With a total photon flux of 4×10 9 photons/s in the range of 30 eV to 100 eV and 1×10 7 photons/s in the range of 100 eV to 200 eV, the resulting quasi-supercontinuum XUV source is suited for applications such as XUV coherence tomography (XCT) or near-edge absorption fine structure spectroscopy (NEXAFS).« less

Multimodal full-field optical coherence tomography on biological tissue: toward all optical digital pathology

NASA Astrophysics Data System (ADS)

Harms, F.; Dalimier, E.; Vermeulen, P.; Fragola, A.; Boccara, A. C.

2012-03-01

Optical Coherence Tomography (OCT) is an efficient technique for in-depth optical biopsy of biological tissues, relying on interferometric selection of ballistic photons. Full-Field Optical Coherence Tomography (FF-OCT) is an alternative approach to Fourier-domain OCT (spectral or swept-source), allowing parallel acquisition of en-face optical sections. Using medium numerical aperture objective, it is possible to reach an isotropic resolution of about 1x1x1 ìm. After stitching a grid of acquired images, FF-OCT gives access to the architecture of the tissue, for both macroscopic and microscopic structures, in a non-invasive process, which makes the technique particularly suitable for applications in pathology. Here we report a multimodal approach to FF-OCT, combining two Full-Field techniques for collecting a backscattered endogeneous OCT image and a fluorescence exogeneous image in parallel. Considering pathological diagnosis of cancer, visualization of cell nuclei is of paramount importance. OCT images, even for the highest resolution, usually fail to identify individual nuclei due to the nature of the optical contrast used. We have built a multimodal optical microscope based on the combination of FF-OCT and Structured Illumination Microscopy (SIM). We used x30 immersion objectives, with a numerical aperture of 1.05, allowing for sub-micron transverse resolution. Fluorescent staining of nuclei was obtained using specific fluorescent dyes such as acridine orange. We present multimodal images of healthy and pathological skin tissue at various scales. This instrumental development paves the way for improvements of standard pathology procedures, as a faster, non sacrificial, operator independent digital optical method compared to frozen sections.

Neurosurgical hand-held optical coherence tomography (OCT) forward-viewing probe

NASA Astrophysics Data System (ADS)

Sun, Cuiru; Lee, Kenneth K. C.; Vuong, Barry; Cusimano, Michael; Brukson, Alexander; Mariampillai, Adrian; Standish, Beau A.; Yang, Victor X. D.

2012-02-01

A prototype neurosurgical hand-held optical coherence tomography (OCT) imaging probe has been developed to provide micron resolution cross-sectional images of subsurface tissue during open surgery. This new ergonomic hand-held probe has been designed based on our group's previous work on electrostatically driven optical fibers. It has been packaged into a catheter probe in the familiar form factor of the clinically accepted Bayonet shaped neurosurgical non-imaging Doppler ultrasound probes. The optical design was optimized using ZEMAX simulation. Optical properties of the probe were tested to yield an ~20 um spot size, 5 mm working distance and a 3.5 mm field of view. The scan frequency can be increased or decreased by changing the applied voltage. Typically a scan frequency of less than 60Hz is chosen to keep the applied voltage to less than 2000V. The axial resolution of the probe was ~15 um (in air) as determined by the OCT system. A custom-triggering methodology has been developed to provide continuous stable imaging, which is crucial for clinical utility. Feasibility of this probe, in combination with a 1310 nm swept source OCT system was tested and images are presented to highlight the usefulness of such a forward viewing handheld OCT imaging probe. Knowledge gained from this research will lay the foundation for developing new OCT technologies for endovascular management of cerebral aneurysms and transsphenoidal neuroendoscopic treatment of pituitary tumors.

Assessment of circumferential angle-closure by the iris-trabecular contact index with swept-source optical coherence tomography.

PubMed

Baskaran, Mani; Ho, Sue-Wei; Tun, Tin A; How, Alicia C; Perera, Shamira A; Friedman, David S; Aung, Tin

2013-11-01

To evaluate the diagnostic performance of the iris-trabecular contact (ITC) index, a measure of the degree of angle-closure, using swept-source optical coherence tomography (SSOCT, CASIA SS-1000, Tomey Corporation, Nagoya, Japan) in comparison with gonioscopy. Prospective observational study. A total of 108 normal subjects and 32 subjects with angle-closure. The SSOCT 3-dimensional angle scans, which obtain radial scans for the entire circumference of the angle, were performed under dark conditions and analyzed using customized software by a single examiner masked to the subjects' clinical details. The ITC index was calculated as a percentage of the angle that was closed on SSOCT images. First-order agreement coefficient (AC1) statistics and area under the receiver operating characteristic curve (AUC) analyses were performed for angle-closure on the basis of the ITC index in comparison with gonioscopy. Angle-closure on gonioscopy was defined as nonvisibility of posterior trabecular meshwork for at least 2 quadrants. Agreement of the ITC index with gonioscopically defined angle-closure was assessed using the AC1 statistic. Study subjects were predominantly Chinese (95.7%) and female (70.7%), with a mean age of 59.2 (standard deviation, 8.9) years. The median ITC index was 15.24% for gonioscopically open-angle eyes (n = 108) and 48.5% for closed-angle eyes (n = 32) (P = 0.0001). The agreement for angle-closure based on ITC index cutoffs (>35% and ≥50%) and gonioscopic angle-closure was 0.699 and 0.718, respectively. The AUC for angle-closure detection using the ITC index was 0.83 (95% confidence interval, 0.76-0.89), with an ITC index >35% having a sensitivity of 71.9% and specificity of 84.3%. The ITC index is a summary measure of the circumferential extent of angle-closure as imaged with SSOCT. The index had moderate agreement and good diagnostic performance for angle-closure with gonioscopy as the reference standard. Copyright © 2013 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved.

Comparison of Keratometry Obtained by a Swept Source OCT-Based Biometer with a Standard Optical Biometer and Scheimpflug Imaging.

PubMed

Asena, Leyla; Akman, Ahmet; Güngör, Sirel Gür; Dursun Altınörs, Dilek

2018-04-09

To assess agreement of a swept source-optical coherence tomography (SS-OCT) based Biometer with a standard IOLMaster device and Scheimpflug Imaging (SI) to acquire keratometric measurements in cataract patients. In this prospective comparative study, 101 eyes of 101 cataract surgery candidates, aged 24-81 years, were sequentially examined using three devices. Keratometry values at the flat (K1) and steep (K2) axis, mean corneal power (Km) and magnitude of corneal astigmatism as well as J0 and J45 vectoral components of astigmatism obtained with the SS-OCT based biometer (IOLMaster 700) were compared with those obtained with the IOLMaster 500 and SI. The agreement between measurements was evaluated by the Bland-Altman method, intraclass correlation coefficients (ICCs) and repeated-measures analysis of variance. Mean K1 values from the three devices were similar (p = 0.09). Mean K2 and Km values of IOLMaster 700 were higher than SI and lower than IOLMaster 500 (p = 0.04 for K2 and p = 0.02 for Km). There was a strong correlation between K1, K2, Km and magnitude of astigmatism obtained with all devices (r ≥ 0.80 and p < 0.01). The 95% limits of agreement (LoA) width for each keratometric value were highest for the comparison between IOLMaster 500 and SI and lowest for the comparison between IOLMaster 700 and 500. The mean differences (width of 95% LoA) for J0 and J45 vectoral components were 0.005 (2.19) and 0.12 (2.92) for the measurements obtained by IOLMaster 700 vs IOLMaster 500 and 0.06 (1.79) and 0.02 (1.58) for the measurements obtained by IOLMaster 700 vs SI, respectively. With ICCs close to 1, the agreement between all devices was excellent for keratometric measurements. Mean K2, Km and astigmatism measurements from IOLMaster 700 were lower than IOLMaster 500 and higher than SI. However, the differences were quite small and are not expected to affect the final IOL power.

Optical coherence tomography angiography in the management of age-related macular degeneration.

PubMed

Schneider, Eric W; Fowler, Samuel C

2018-05-01

Optical coherence tomography angiography (OCT-A) provides rapid, flow-based imaging of the retinal and choroidal vasculature in a noninvasive manner. This review contrasts this novel technique with conventional angiography and discusses its current uses and limitations in the management of age-related macular degeneration (AMD). Initial work with OCT-A has focused on its ability to identify choriocapillaris flow alterations in dry AMD and to sensitively detect choroidal neovascular membranes (CNVs) in neovascular AMD. Reduced choriocapillaris flow beyond the borders of geographic atrophy seen on OCT-A suggests a primary vascular cause in geographic atrophy. Longitudinal OCT-A analysis of CNV morphology has demonstrated the transition from an immature to mature CNV phenotype following treatment. Current clinical applications of OCT-A include identification of asymptomatic CNV and monitoring for CNV development in the setting of an acquired vitelliform lesion. OCT-A remains a promising diagnostic tool but one still very much in evolution. Larger studies will be needed to more accurately describe its sensitivity and specificity for CNV detection and to better characterize longitudinal CNV morphologic changes. Anticipated hardware and software updates including swept-source light sources, automated montaging, and manual adjustment of interscan timing should enhance the capabilities of OCT-A in the management of AMD.

High-speed high-sensitivity infrared spectroscopy using mid-infrared swept lasers (Conference Presentation)

NASA Astrophysics Data System (ADS)

Childs, David T. D.; Groom, Kristian M.; Hogg, Richard A.; Revin, Dmitry G.; Cockburn, John W.; Rehman, Ihtesham U.; Matcher, Stephen J.

2016-03-01

Infrared spectroscopy is a highly attractive read-out technology for compositional analysis of biomedical specimens because of its unique combination of high molecular sensitivity without the need for exogenous labels. Traditional techniques such as FTIR and Raman have suffered from comparatively low speed and sensitivity however recent innovations are challenging this situation. Direct mid-IR spectroscopy is being speeded up by innovations such as MEMS-based FTIR instruments with very high mirror speeds and supercontinuum sources producing very high sample irradiation levels. Here we explore another possible method - external cavity quantum cascade lasers (EC-QCL's) with high cavity tuning speeds (mid-IR swept lasers). Swept lasers have been heavily developed in the near-infrared where they are used for non-destructive low-coherence imaging (OCT). We adapt these concepts in two ways. Firstly by combining mid-IR quantum cascade gain chips with external cavity designs adapted from OCT we achieve spectral acquisition rates approaching 1 kHz and demonstrate potential to reach 100 kHz. Secondly we show that mid-IR swept lasers share a fundamental sensitivity advantage with near-IR OCT swept lasers. This makes them potentially able to achieve the same spectral SNR as an FTIR instrument in a time x N shorter (N being the number of spectral points) under otherwise matched conditions. This effect is demonstrated using measurements of a PDMS sample. The combination of potentially very high spectral acquisition rates, fundamental SNR advantage and the use of low-cost detector systems could make mid-IR swept lasers a powerful technology for high-throughput biomedical spectroscopy.

Evaluation of Tizian overlays by means of a swept source optical coherence tomography system

NASA Astrophysics Data System (ADS)

Marcauteanu, Corina; Sinescu, Cosmin; Negrutiu, Meda Lavinia; Stoica, Eniko Tunde; Topala, Florin; Duma, Virgil Florin; Bradu, Adrian; Podoleanu, Adrian Gh.

2016-03-01

The teeth affected by pathologic attrition can be restored by a minimally invasive approach, using Tizian overlays. In this study we prove the advantages of a fast swept source (SS) OCT system in the evaluation of Tizian overlays placed in an environment characterized by high occlusal forces. 12 maxillary first premolars were extracted and prepared for overlays. The Tizian overlays were subjected to 3000 alternating cycles of thermo-cycling (from -10°C to +50°C) and to mechanical occlusal overloads (at 800 N). A fast SS OCT system was used to evaluate the Tizian overlays before and after the mechanical and thermal straining. The SS (Axsun Technologies, Billerica, MA) has a central wavelength of 1060 nm, sweeping range of 106 nm (quoted at 10 dB) and a 100 kHz line rate. The depth resolution of the system, measured experimentally in air was 10 μm. The imaging system used for this study offers high spatial resolutions in both directions, transversal and longitudinal of around 10 μm, a high sensitivity, and it is also able to acquire entire tridimensional (3D)/volume reconstructions as fast as 2.5 s. Once the full dataset was acquired, rendered high resolutions en-face projections could be produced. Using them, the overlay (i.e., cement) abutment tooth interfaces were remarked both on B-scans/two-dimensional (2D) sections and in the 3D reconstructions. Using the system several open interfaces were possible to detect. The fast SS OCT system thus proves useful in the evaluation of zirconia reinforced composite overlays, placed in an environment characterized by high occlusal forces.

In vivo microcirculation imaging of the sub surface fingertip using correlation mapping optical coherence tomography (cmOCT)

NASA Astrophysics Data System (ADS)

Dsouza, Roshan I.; Zam, Azhar; Subhash, Hrebesh M.; Larin, Kirill V.; Leahy, Martin

2013-02-01

We describe a novel application of correlation mapping optical coherence tomography (cmOCT) for sub-surface fingerprint biometric identification. Fingerprint biometrics including automated fingerprint identification systems, are commonly used to recognise the fingerprint, since they constitute simple, effective and valuable physical evidence. Spoofing of biometric fingerprint devices can be easily done because of the limited information obtained from the surface topography. In order to overcome this limitation a potentially more secure source of information is required for biometric identification applications. In this study, we retrieve the microcirculation map of the subsurface fingertip by use of the cmOCT technique. To increase probing depth of the sub surface microcirculation, an optical clearing agent composed of 75% glycerol in aqueous solution was applied topically and kept in contact for 15 min. OCT intensity images were acquired from commercial research grade swept source OCT system (model OCT1300SS, Thorlabs Inc. USA). A 3D OCT scan of the fingertip was acquired over an area of 5x5 mm using 1024x1024 A-scans in approximately 70 s. The resulting volume was then processed using the cmOCT technique with a 7x7 kernel to provide a microcirculation map. We believe these results will demonstrate an enhanced security level over artificial fingertips. To the best of our knowledge, this is the first demonstration of imaging microcirculation map of the subsurface fingertip.

Investigation of multiple optical and biometric properties of optic nerve head (Conference Presentation)

NASA Astrophysics Data System (ADS)

Hong, Young-Joo; Chan, Aaron C.; Kasaragod, Deepa K.; Makita, Shuichi; Miura, Masahiro; Yasuno, Yoshiaki

2017-02-01

Glaucoma is a group of eye diseases which results in optic nerve damage and vision loss. Optical coherence tomography (OCT) has been widely used to investigate geometric risk factor of glaucoma. However, material properties of ONH are also important to understand intra-ocular pressure related stress. We developed Jones-matrix based multifunctional posterior eye OCT (JM-OCT), which uses 1-μm band swept-source with a 100-kHz A-line rate. It provides three different optical properties, attenuation coefficient (AC), local birefringence (LB), and optical coherence angiography (OCA). We investigated the utility those properties for the investigation of normal ONH cases. 3 mm x 3 mm area around ONH was scanned for each eye, and biometric parameters were measured in hospital. Statistical analyses were performed with the mean values of above parameters at the regions of prelamina, lamina cribrosa, peripapillary sclera, and peripapillary nerve fiber layer, and biometric parameters of age, axial eye length, refractive error, and intraocular pressure. In qualitative observation, the lamina cribrosa generally shows more hyper signals in AC, LB, and OCA than prelamina. In t-test, AC, LB, and OCA showed significant difference (p < 0.05) between prelamina and lamina cribrosa, while conventional OCT did not. In correlation test, axial eye length is positively correlated with LB and AC in lamina cribrosa. And these LB and AC are also negatively correlated with the refractive error. Age was found to be negatively correlated with OCA in lamina cribrosa.

Transient response of nonlinear magneto-optic rotation in a paraffin-coated Rb vapor cell

DOE Office of Scientific and Technical Information (OSTI.GOV)

Momeen, M. Ummal; Rangarajan, G.; Natarajan, Vasant

2010-01-15

We study resonant nonlinear magneto-optic rotation (NMOR) in a paraffin-coated Rb vapor cell as the magnetic field is swept. At low sweep rates, the nonlinear rotation appears as a narrow resonance signal with a linewidth of about '300 muG' (2pix420 Hz). At high sweep rates, the signal shows transient response with an oscillatory decay. The decay time constant is of order 100 ms. The behavior is different for transitions starting from the lower or the upper hyperfine level of the ground state because of optical pumping effects.

Characterization of wavelength-swept active mode locking fiber laser based on reflective semiconductor optical amplifier

NASA Astrophysics Data System (ADS)

Lee, Hwi Don; Lee, Ju Han; Yung Jeong, Myung; Kim, Chang-Seok

2011-07-01

The static and dynamic characteristics of a wavelength-swept active mode locking (AML) fiber laser are presented in both the time-region and wavelength-region. This paper shows experimentally that the linewidth of a laser spectrum and the bandwidth of the sweeping wavelength are dependent directly on the length and dispersion of the fiber cavity as well as the modulation frequency and sweeping rate under the mode-locking condition. To achieve a narrower linewidth, a longer length and higher dispersion of the fiber cavity as well as a higher order mode locking condition are required simultaneously. For a broader bandwidth, a lower order of the mode locking condition is required using a lower modulation frequency. The dynamic sweeping performance is also analyzed experimentally to determine its applicability to optical coherence tomography imaging. It is shown that the maximum sweeping rate can be improved by the increased free spectral range from the shorter length of the fiber cavity. A reflective semiconductor optical amplifier (RSOA) was used to enhance the modulation and dispersion efficiency. Overall a triangular electrical signal can be used instead of the sinusoidal signal to sweep the lasing wavelength at a high sweeping rate due to the lack of mechanical restrictions in the wavelength sweeping mechanism.

Wideband Electrically-Pumped 1050 nm MEMS-Tunable VCSEL for Ophthalmic Imaging.

PubMed

John, Demis D; Burgner, Christopher B; Potsaid, Benjamin; Robertson, Martin E; Lee, Byung Kun; Choi, Woo Jhon; Cable, Alex E; Fujimoto, James G; Jayaraman, Vijaysekhar

2015-08-15

In this paper, we present a 1050 nm electrically-pumped micro-electro-mechanically-tunable vertical-cavity-surface-emitting-laser (MEMS-VCSEL) with a record dynamic tuning bandwidth of 63.8 nm, suitable for swept source optical coherence tomography (SS-OCT) imaging. These devices provide reduced cost & complexity relative to previously demonstrated optically pumped devices by obviating the need for a pump laser and associated hardware. We demonstrate ophthalmic SS-OCT imaging with the electrically-pumped MEMS-VCSEL at a 400 kHz axial scan rate for wide field imaging of the in vivo human retina over a 12 mm × 12 mm field and for OCT angiography of the macula over 6 mm × 6 mm & 3 mm × 3 mm fields to show retinal vasculature and capillary structure near the fovea. These results demonstrate the feasibility of electrically pumped MEMS-VCSELs in ophthalmic instrumentation, the largest clinical application of OCT. In addition, we estimate that the 3 dB coherence length in air is 225 meters ± 51 meters, far greater than required for ophthalmic SS-OCT and suggestive of other distance ranging applications.

Automatic characterization and segmentation of human skin using three-dimensional optical coherence tomography

NASA Astrophysics Data System (ADS)

Hori, Yasuaki; Yasuno, Yoshiaki; Sakai, Shingo; Matsumoto, Masayuki; Sugawara, Tomoko; Madjarova, Violeta; Yamanari, Masahiro; Makita, Shuichi; Yasui, Takeshi; Araki, Tsutomu; Itoh, Masahide; Yatagai, Toyohiko

2006-03-01

A set of fully automated algorithms that is specialized for analyzing a three-dimensional optical coherence tomography (OCT) volume of human skin is reported. The algorithm set first determines the skin surface of the OCT volume, and a depth-oriented algorithm provides the mean epidermal thickness, distribution map of the epidermis, and a segmented volume of the epidermis. Subsequently, an en face shadowgram is produced by an algorithm to visualize the infundibula in the skin with high contrast. The population and occupation ratio of the infundibula are provided by a histogram-based thresholding algorithm and a distance mapping algorithm. En face OCT slices at constant depths from the sample surface are extracted, and the histogram-based thresholding algorithm is again applied to these slices, yielding a three-dimensional segmented volume of the infundibula. The dermal attenuation coefficient is also calculated from the OCT volume in order to evaluate the skin texture. The algorithm set examines swept-source OCT volumes of the skins of several volunteers, and the results show the high stability, portability and reproducibility of the algorithm.

Ultrahigh speed endoscopic optical coherence tomography using micromotor imaging catheter and VCSEL technology.

PubMed

Tsai, Tsung-Han; Potsaid, Benjamin; Tao, Yuankai K; Jayaraman, Vijaysekhar; Jiang, James; Heim, Peter J S; Kraus, Martin F; Zhou, Chao; Hornegger, Joachim; Mashimo, Hiroshi; Cable, Alex E; Fujimoto, James G

2013-07-01

We developed a micromotor based miniature catheter with an outer diameter of 3.2 mm for ultrahigh speed endoscopic swept source optical coherence tomography (OCT) using a vertical cavity surface-emitting laser (VCSEL) at a 1 MHz axial scan rate. The micromotor can rotate a micro-prism at several hundred frames per second with less than 5 V drive voltage to provide fast and stable scanning, which is not sensitive to the bending of the catheter. The side-viewing probe can be pulled back to acquire a three-dimensional (3D) data set covering a large area on the specimen. The VCSEL provides a high axial scan rate to support dense sampling under high frame rate operation. Using a high speed data acquisition system, in vivo 3D-OCT imaging in the rabbit GI tract and ex vivo imaging of a human colon specimen with 8 μm axial resolution, 8 μm lateral resolution and 1.2 mm depth range in tissue at a frame rate of 400 fps was demonstrated.

Ultrahigh speed endoscopic optical coherence tomography using micromotor imaging catheter and VCSEL technology

PubMed Central

Tsai, Tsung-Han; Potsaid, Benjamin; Tao, Yuankai K.; Jayaraman, Vijaysekhar; Jiang, James; Heim, Peter J. S.; Kraus, Martin F.; Zhou, Chao; Hornegger, Joachim; Mashimo, Hiroshi; Cable, Alex E.; Fujimoto, James G.

2013-01-01

We developed a micromotor based miniature catheter with an outer diameter of 3.2 mm for ultrahigh speed endoscopic swept source optical coherence tomography (OCT) using a vertical cavity surface-emitting laser (VCSEL) at a 1 MHz axial scan rate. The micromotor can rotate a micro-prism at several hundred frames per second with less than 5 V drive voltage to provide fast and stable scanning, which is not sensitive to the bending of the catheter. The side-viewing probe can be pulled back to acquire a three-dimensional (3D) data set covering a large area on the specimen. The VCSEL provides a high axial scan rate to support dense sampling under high frame rate operation. Using a high speed data acquisition system, in vivo 3D-OCT imaging in the rabbit GI tract and ex vivo imaging of a human colon specimen with 8 μm axial resolution, 8 μm lateral resolution and 1.2 mm depth range in tissue at a frame rate of 400 fps was demonstrated. PMID:23847737

Ultrahigh-speed non-invasive widefield angiography

NASA Astrophysics Data System (ADS)

Blatter, Cedric; Klein, Thomas; Grajciar, Branislav; Schmoll, Tilman; Wieser, Wolfgang; Andre, Raphael; Huber, Robert; Leitgeb, Rainer A.

2012-07-01

Retinal and choroidal vascular imaging is an important diagnostic benefit for ocular diseases such as age-related macular degeneration. The current gold standard for vessel visualization is fluorescence angiography. We present a potential non-invasive alternative to image blood vessels based on functional Fourier domain optical coherence tomography (OCT). For OCT to compete with the field of view and resolution of angiography while maintaining motion artifacts to a minimum, ultrahigh-speed imaging has to be introduced. We employ Fourier domain mode locking swept source technology that offers high quality imaging at an A-scan rate of up to 1.68 MHz. We present retinal angiogram over ˜48 deg acquired in a few seconds in a single recording without the need of image stitching. OCT at 1060 nm allows for high penetration in the choroid and efficient separate characterization of the retinal and choroidal vascularization.

All-fiber wavelength swept ring laser based on Fabry-Perot filter for optical frequency domain imaging

PubMed Central

Jun, Changsu; Villiger, Martin; Oh, Wang-Yuhl; Bouma, Brett E.

2014-01-01

Innovations in laser engineering have yielded several novel configurations for high repetition rate, broad sweep range, and long coherence length wavelength swept lasers. Although these lasers have enabled high performance frequency-domain optical coherence tomography, they are typically complicated and costly and many require access to proprietary materials or devices. Here, we demonstrate a simplified ring resonator configuration that is straightforward to construct from readily available materials at a low total cost. It was enabled by an insight regarding the significance of isolation against bidirectional operation and by configuring the sweep range of the intracavity filter to exceed its free spectral range. The design can easily be optimized to meet a range of operating specifications while yielding robust and stable performance. As an example, we demonstrate 240 kHz operation with 125 nm sweep range and >70 mW of average output power and demonstrate high quality frequency domain OCT imaging. The complete component list and directions for assembly of the laser are posted on-line at www.octresearch.org. PMID:25401614

Measuring the thickness of the peritoneal membrane in mice with optical coherence tomography

NASA Astrophysics Data System (ADS)

Alwafi, Reem; Dickinson, Mark; Brenchley, Paul; Walkin, Louise

2011-06-01

The detection and diagnosis of diseases have improved in recent years. Developments in diagnostic techniques have helped to improve treatment in the early stages and to avoid many risks to patients. One such technique is optical coherence tomography (OCT), which is used in many medical applications to perform internal microstructural imaging of the human body at high resolution (typically 10 μm), at high speed and in real time. OCT is non-invasive and can be used as a contact or non-contact technique to obtain an image. In medicine, there are many applications that involve OCT, such as in ophthalmology, gastroenterology, cardiology and oncology. This work demonstrates the use of an OCT system incorporating a swept laser source with a high sweep rate of 16 kHz over a wide range of wavelengths (1260 nm to 1390 nm) to measure the thickness of the peritoneal membrane in mice of different sizes and weights. The real axial line speed is limited by the source that is used in the OCT system. The optical source has a bandwidth of ▵λ =110 nm, centred at λ0 =1325 nm. The aim of this study is to investigate the thickening of the peritoneal membrane which can occur during prolonged peritoneal dialysis in mice. As part of this preliminary study, healthy mice of different weights were euthanized and the thickness of the peritoneal membrane was measured using OCT. The aim was to gather data on the expected range of thicknesses present in healthy animals for future studies. For this work, two locations on the peritoneal membrane of each of 20 mice were imaged.

Comparison of Tone Mode Measurements for a Forward Swept and Baseline Rotor Fan

NASA Technical Reports Server (NTRS)

Heidelberg, Laurence J.

2003-01-01

A forward swept fan, designated the Quite High Speed Fan (QHSF), was tested in the NASA Glenn 9- by 15-foot Low Speed Wind Tunnel to investigate its noise reduction relative to a baseline fan of the same aerodynamic performance. The design objective of the QHSF was a 6 dB reduction in Effective Perceived Noise Level relative to the baseline fan at the takeoff condition. The design noise reduction was to be a result of lower levels of multiple pure tone noise due to the forward swept rotor, and lower rotor/stator interaction tone noise from a leaned stator. Although the design 6 dB reduction was observed in far-field measurements, the induct mode measurements revealed the reasons for goals. All of the noise reduction was from the blade passing tone and its harmonics and most of this was unexpectedly from rotor/strut interaction modes. The reason for large differences in rotor/strut noise sources could not be determined with certainty. The reductions in the multiple pure tone noise for the forward swept rotor were not observed. this reduction were not the ones related to the design

Advances of optical coherence tomography in myopia and pathologic myopia

PubMed Central

Ng, D S C; Cheung, C Y L; Luk, F O; Mohamed, S; Brelen, M E; Yam, J C S; Tsang, C W; Lai, T Y Y

2016-01-01

The natural course of high-axial myopia is variable and the development of pathologic myopia is not fully understood. Advancements in optical coherence tomography (OCT) technology have revealed peculiar intraocular structures in highly myopic eyes and unprecedented pathologies that cause visual impairment. New OCT findings include posterior precortical vitreous pocket and precursor stages of posterior vitreous detachment; peripapillary intrachoroidal cavitation; morphological patterns of scleral inner curvature and dome-shaped macula. Swept source OCT is capable of imaging deeper layers in the posterior pole for investigation of optic nerve pits, stretched and thinned lamina cribrosa, elongated dural attachment at posterior scleral canal, and enlargement of retrobulbar subarachnoid spaces. This has therefore enabled further evaluation of various visual field defects in high myopia and the pathogenesis of glaucomatous optic neuropathy. OCT has many potential clinical uses in managing visual impairing conditions in pathologic myopia. Understanding how retinal nerve fibers are redistributed in axial elongation will allow the development of auto-segmentation software for diagnosis and monitoring progression of glaucoma. OCT is indispensable in the diagnosis of various conditions associated with myopic traction maculopathy and monitoring of post-surgical outcomes. In addition, OCT is commonly used in the multimodal imaging assessment of myopic choroidal neovascularization. Biometry and topography of the retinal layers and choroid will soon be validated for the classification of myopic maculopathy for utilization in epidemiological studies as well as clinical trials. PMID:27055674

Observation of neovascularization of the disc associated with proliferative diabetic retinopathy using OCT angiography.

PubMed

Akiyama, Hideo; Li, Danjie; Shimoda, Yukitoshi; Matsumoto, Hidetaka; Kishi, Shoji

2018-05-01

To describe the relationship between the vitreous and the neovascularization of the disc (NVD) using swept source optical coherence tomography (SS-OCT) and OCT angiography (OCTA). Retrospective. We examined 17 eyes of 11 consecutive patients diagnosed as NVD associated with proliferative diabetic retinopathy (PDR). The location of the NVD feeder or collector vessels were examined by using RTVue XR Avanti. To determine the condition of the posterior vitreous detachment (PVD) and the proliferative tissue of the NVD, we performed 12 mm horizontal and vertical scans through the disc using SS-OCT. OCT images of all 17 cases indicated there was no PVD on the optic disc. OCTA showed that the locations of the newly formed vessels from the optic disc were overwhelmingly outside the physiological cupping (95%). No cases exhibited formation of neovascularization inside the physiological cupping. OCT images revealed all 17 eyes had proliferative tissues located under the posterior wall of the vitreous, with 12 out of 17 eyes exhibiting additional invasion of the proliferative tissue into the vitreous through the posterior wall. Epiretinal membrane or a thickened posterior wall of the vitreous was present in 10 out of the 17 eyes. NVD associated with PDR arises from outside the physiological cupping and grows along the posterior wall of the vitreous. The absence of PVD on the optic disc is essential to the growth of NVD.

A large-scan-angle piezoelectric MEMS optical scanner actuated by a Nb-doped PZT thin film

NASA Astrophysics Data System (ADS)

Naono, Takayuki; Fujii, Takamichi; Esashi, Masayoshi; Tanaka, Shuji

2014-01-01

Resonant 1D microelectromechanical systems (MEMS) optical scanners actuated by piezoelectric unimorph actuators with a Nb-doped lead zirconate titanate (PNZT) thin film were developed for endoscopic optical coherence tomography (OCT) application. The MEMS scanners were designed as the resonance frequency was less than 125 Hz to obtain enough pixels per frame in OCT images. The device size was within 3.4 mm × 2.5 mm, which is compact enough to be installed in a side-imaging probe with 4 mm inner diameter. The fabrication process started with a silicon-on-insulator wafer, followed by PNZT deposition by the Rf sputtering and Si bulk micromachining process. The fabricated MEMS scanners showed maximum optical scan angles of 146° at 90 Hz, 148° at 124 Hz, 162° at 180 Hz, and 152° at 394 Hz at resonance in atmospheric pressure. Such wide scan angles were obtained by a drive voltage below 1.3 Vpp, ensuring intrinsic safety in in vivo uses. The scanner with the unpoled PNZT film showed three times as large a scan angle as that with a poled PZT films. A swept-source OCT system was constructed using the fabricated MEMS scanner, and cross-sectional images of a fingertip with image widths of 4.6 and 2.3 mm were acquired. In addition, a PNZT-based angle sensor was studied for feedback operation.

Laser skin friction measurements and CFD comparison of weak-to-strong swept shock/boundary-layer interactions

NASA Technical Reports Server (NTRS)

Kim, K.-S.; Lee, Y.; Alvi, F. S.; Settles, G. S.; Horstman, C. C.

1990-01-01

A joint experimental and computational study of skin friction in weak-to-strong swept shock wave/turbulent boundary-layer interactions has been carried out. A planar shock wave is generated by a sharp fin at angles of attack alpha = 10 deg and 16 deg at M(infinity) = 3 and 16 and 20 deg at M(infinity) = 4. Measurements are made using the Laser Interferometer Skin Friction meter, which optically detects the rate of thinning of an oil film applied to the test surface. The results show a systematic rise in the peak c(f) at the rear part of the interaction, where the separated flow atttaches. For the stronget case studied, this peak is an order of magnitude higher than the incoming freestream c(f)level.

Experimental investigation of a supersonic swept ramp injector using laser-induced iodine fluorescence

NASA Technical Reports Server (NTRS)

Hartfield, Roy J.; Hollo, Steven D.; Mcdaniel, James C.

1990-01-01

Planar measurements of injectant mole fraction and temperature have been conducted in a nonreacting supersonic combustor configured with underexpanded injection in the base of a swept ramp. The temperature measurements were conducted with a Mach 2 test section inlet in streamwise planes perpendicular to the test section wall on which the ramp was mounted. Injection concentration measurements, conducted in cross flow planes with both Mach 2 and Mach 2.9 free stream conditions, dramatically illustrate the domination of the mixing process by streamwise vorticity generated by the ramp. These measurements, conducted using a nonintrusive optical technique (laser-induced iodine fluorescence), provide an accurate and extensive experimental data base for the validation of computation fluid dynamic codes for the calculation of highly three-dimensional supersonic combustor flow fields.

Dual-wavelength photothermal optical coherence tomography for blood oxygen saturation measurement

NASA Astrophysics Data System (ADS)

Yin, Biwei; Kuranov, Roman V.; McElroy, Austin B.; Milner, Thomas E.

2013-03-01

We report design and demonstration of a dual wavelength photothermal (DWP) optical coherence tomography (OCT) system for imaging of a phantom microvessel and measurement of hemoglobin oxygen saturation (SO2) level. The DWP-OCT system contains a swept-source (SS) two-beam phase-sensitive (PhS) OCT system (1060 nm) and two intensity modulated photothermal excitation lasers (770 nm and 800 nm). The PhS-OCT probe beam (1060 nm) and photothermal excitation beams are combined into one single-mode optical fiber. A galvanometer based two-dimensional achromatic scanning system is designed to provide 14 μm lateral resolution for the PhS-OCT probe beam (1060 nm) and 13 μm lateral resolution for photothermal excitation beams. DWP-OCT system's sensitivity is 102 dB, axial resolution is 13 μm in tissue and uses a real-time digital dispersion compensation algorithm. Noise floor for optical pathlength measurements is 300 pm in the signal frequency range (380-400 Hz) of photothermal modulation frequencies. Blood SO2 level is calculated from measured optical pathlength (op) signal in a 300 μm diameter microvessel phantom introduced by the two photothermal excitation beams. En-face and B-scan images of a phantom microvessel are recorded, and six blood samples' SO2 levels are measured using DWP-OCT and compared with values provided by a commercial blood oximeter. A mathematical model indicates thermal diffusion introduces a systematic artifact that over-estimates SO2 values and is consistent with measured data.

Adaptive Optics Optical Coherence Tomography in Glaucoma

PubMed Central

Dong, Zachary M.; Wollstein, Gadi; Wang, Bo; Schuman, Joel S.

2016-01-01

Since the introduction of commercial optical coherence tomography (OCT) systems, the ophthalmic imaging modality has rapidly expanded and it has since changed the paradigm of visualization of the retina and revolutionized the management and diagnosis of neuro-retinal diseases, including glaucoma. OCT remains a dynamic and evolving imaging modality, growing from time-domain OCT to the improved spectral-domain OCT, adapting novel image analysis and processing methods, and onto the newer swept-source OCT and the implementation of adaptive optics (AO) into OCT. The incorporation of AO into ophthalmic imaging modalities has enhanced OCT by improving image resolution and quality, particularly in the posterior segment of the eye. Although OCT previously captured in-vivo cross-sectional images with unparalleled high resolution in the axial direction, monochromatic aberrations of the eye limit transverse or lateral resolution to about 15-20 μm and reduce overall image quality. In pairing AO technology with OCT, it is now possible to obtain diffraction-limited resolution images of the optic nerve head and retina in three-dimensions, increasing resolution down to a theoretical 3 μm3. It is now possible to visualize discrete structures within the posterior eye, such as photoreceptors, retinal nerve fiber layer bundles, the lamina cribrosa, and other structures relevant to glaucoma. Despite its limitations and barriers to widespread commercialization, the expanding role of AO in OCT is propelling this technology into clinical trials and onto becoming an invaluable modality in the clinician's arsenal. PMID:27916682

Design and testing of prototype handheld scanning probes for optical coherence tomography

PubMed Central

Demian, Dorin; Sinescu, Cosmin; Negrutiu, Meda Lavinia; Cernat, Ramona; Topala, Florin Ionel; Hutiu, Gheorghe; Bradu, Adrian; Podoleanu, Adrian Gh

2014-01-01

Three simple and low-cost configurations of handheld scanning probes for optical coherence tomography have been developed. Their design and testing for dentistry applications are presented. The first two configurations were built exclusively from available off-the-shelf optomechanical components, which, to the best of our knowledge, are the first designs of this type. The third configuration includes these components in an optimized and ergonomic probe. All the designs are presented in detail to allow for their duplication in any laboratory with a minimum effort, for applications that range from educational to high-end clinical investigations. Requirements that have to be fulfilled to achieve configurations which are reliable, ergonomic—for clinical environments, and easy to build are presented. While a range of applications is possible for the prototypes developed, in this study the handheld probes are tested ex vivo with a spectral domain optical coherence tomography system built in-house, for dental constructs. A previous testing with a swept source optical coherence tomography system has also been performed both in vivo and ex vivo for ear, nose, and throat—in a medical environment. The applications use the capability of optical coherence tomography to achieve real-time, high-resolution, non-contact, and non-destructive interferometric investigations with micrometer resolutions and millimeter penetration depth inside the sample. In this study, testing the quality of the material of one of the most used types of dental prosthesis, metalo-ceramic is thus demonstrated. PMID:25107512

Design and testing of prototype handheld scanning probes for optical coherence tomography.

PubMed

Demian, Dorin; Duma, Virgil-Florin; Sinescu, Cosmin; Negrutiu, Meda Lavinia; Cernat, Ramona; Topala, Florin Ionel; Hutiu, Gheorghe; Bradu, Adrian; Podoleanu, Adrian Gh

2014-08-01

Three simple and low-cost configurations of handheld scanning probes for optical coherence tomography have been developed. Their design and testing for dentistry applications are presented. The first two configurations were built exclusively from available off-the-shelf optomechanical components, which, to the best of our knowledge, are the first designs of this type. The third configuration includes these components in an optimized and ergonomic probe. All the designs are presented in detail to allow for their duplication in any laboratory with a minimum effort, for applications that range from educational to high-end clinical investigations. Requirements that have to be fulfilled to achieve configurations which are reliable, ergonomic-for clinical environments, and easy to build are presented. While a range of applications is possible for the prototypes developed, in this study the handheld probes are tested ex vivo with a spectral domain optical coherence tomography system built in-house, for dental constructs. A previous testing with a swept source optical coherence tomography system has also been performed both in vivo and ex vivo for ear, nose, and throat-in a medical environment. The applications use the capability of optical coherence tomography to achieve real-time, high-resolution, non-contact, and non-destructive interferometric investigations with micrometer resolutions and millimeter penetration depth inside the sample. In this study, testing the quality of the material of one of the most used types of dental prosthesis, metalo-ceramic is thus demonstrated. © IMechE 2014.

Scattering angle resolved optical coherence tomography for in vivo murine retinal imaging

NASA Astrophysics Data System (ADS)

Gardner, Michael R.; Katta, Nitesh; McElroy, Austin; Baruah, Vikram; Rylander, H. G.; Milner, Thomas E.

2017-02-01

Optical coherence tomography (OCT) retinal imaging contributes to understanding central nervous system (CNS) diseases because the eye is an anatomical "window to the brain" with direct optical access to nonmylenated retinal ganglion cells. However, many CNS diseases are associated with neuronal changes beyond the resolution of standard OCT retinal imaging systems. Though studies have shown the utility of scattering angle resolved (SAR) OCT for particle sizing and detecting disease states ex vivo, a compact SAR-OCT system for in vivo rodent retinal imaging has not previously been reported. We report a fiber-based SAR-OCT system (swept source at 1310 nm +/- 65 nm, 100 kHz scan rate) for mouse retinal imaging with a partial glass window (center aperture) for angular discrimination of backscattered light. This design incorporates a dual-axis MEMS mirror conjugate to the ocular pupil plane and a high collection efficiency objective. A muring retina is imaged during euthanasia, and the proposed SAR-index is examined versus time. Results show a positive correlation between the SAR-index and the sub-cellular hypoxic response of neurons to isoflurane overdose during euthanasia. The proposed SAR-OCT design and image process technique offer a contrast mechanism able to detect sub-resolution neuronal changes for murine retinal imaging.

In vitro and clinical evaluation of optical coherence tomography for the detection of subgingival calculus and root cementum.

PubMed

Tsubokawa, Masaki; Aoki, Akira; Kakizaki, Sho; Taniguchi, Yoichi; Ejiri, Kenichiro; Mizutani, Koji; Koshy, Geena; Akizuki, Tatsuya; Oda, Shigeru; Sumi, Yasunori; Izumi, Yuichi

2018-05-24

This study evaluated the effectiveness of swept-source optical coherence tomography (ss-OCT) for detecting calculus and root cementum during periodontal therapy. Optical coherence tomography (OCT) images were taken before and after removal of subgingival calculus from extracted teeth and compared with non-decalcified histological sections. Porcine gingival sheets of various thicknesses were applied to the root surfaces of extracted teeth with calculus and OCT images were taken. OCT images were also taken before and after scaling and root planing (SRP) in human patients. In vitro, calculus was clearly detected as a white-gray amorphous structure on the root surface, which disappeared after removal. Cementum was identified as a thin, dark-gray layer. The calculus could not be clearly observed when soft tissues were present on the root surface. Clinically, supragingival calculus and cementum could be detected clearly with OCT, and subgingival calculus in the buccal cervical area of the anterior and premolar teeth was identified, which disappeared after SRP. Digital processing of the original OCT images was useful for clarifying the calculus. In conclusion, ss-OCT showed potential as a periodontal diagnostic tool for detecting cementum and subgingival calculus, although the practical applications of subgingival imaging remain limited.

Optical coherence tomography – current and future applications

PubMed Central

Adhi, Mehreen; Duker, Jay S.

2013-01-01

Purpose of review Optical coherence tomography (OCT) has revolutionized the clinical practice of ophthalmology. It is a noninvasive imaging technique that provides high-resolution, cross-sectional images of the retina, retinal nerve fiber layer and the optic nerve head. This review discusses the present applications of the commercially available spectral-domain OCT (SD-OCT) systems in the diagnosis and management of retinal diseases, with particular emphasis on choroidal imaging. Future directions of OCT technology and their potential clinical uses are discussed. Recent findings Analysis of the choroidal thickness in healthy eyes and disease states such as age-related macular degeneration, central serous chorioretinopathy, diabetic retinopathy and inherited retinal dystrophies has been successfully achieved using SD-OCT devices with software improvements. Future OCT innovations such as longer-wavelength OCT systems including the swept-source technology, along with Doppler OCT and en-face imaging, may improve the detection of subtle microstructural changes in chorioretinal diseases by improving imaging of the choroid. Summary Advances in OCT technology provide for better understanding of pathogenesis, improved monitoring of progression and assistance in quantifying response to treatment modalities in diseases of the posterior segment of the eye. Further improvements in both hardware and software technologies should further advance the clinician’s ability to assess and manage chorioretinal diseases. PMID:23429598

Corneal elastic anisotropy and hysteresis as a function of IOP assessed by optical coherence elastography

NASA Astrophysics Data System (ADS)

Li, Jiasong; Singh, Manmohan; Han, Zhaolong; Wu, Chen; Raghunathan, Raksha; Liu, Chih-Hao; Nair, Achuth; Noorani, Shezaan; Aglyamov, Salavat R.; Twa, Michael D.; Larin, Kirill V.

2016-03-01

The mechanical anisotropic properties of the cornea can be an important indicator for determining the onset and severity of different diseases and can be used to assess the efficacy of various therapeutic interventions, such as cross-linking and LASIK surgery. In this work, we introduce a noncontact method of assessing corneal mechanical anisotropy as a function of intraocular pressure (IOP) using optical coherence elastography (OCE). A focused air-pulse induced low amplitude (<10 μm) elastic waves in fresh porcine corneas in the whole eye-globe configuration in situ. A phase-stabilized swept source optical coherence elastography (PhS-SSOCE) system imaged the elastic wave propagation at stepped radial angles, and the OCE measurements were repeated as the IOP was cycled. The elastic wave velocity was then quantified to determine the mechanical anisotropy and hysteresis of the cornea. The results show that the elastic anisotropy at the corneal of the apex of the cornea becomes more pronounced at higher IOPs, and that there are distinct radial angles of higher and lower stiffness. Due to the noncontact nature and small amplitude of the elastic wave, this method may be useful for characterizing the elastic anisotropy of ocular and other tissues in vivo completely noninvasively.

Investigation of stress-induced birefringence of tissue determined with polarisation sensitive optical coherence tomography (Conference Presentation)

NASA Astrophysics Data System (ADS)

Karnowski, Karol; Li, Qingyun; Villiger, Martin; Sampson, David D.

2017-02-01

Polarisation sensitive optical coherence tomography (PS-OCT) offers additional intrinsic contrast to probe differences between healthy tissue and cancer that are often barely visible due to limited scattering contrast in an OCT image. PS-OCT reconstructs tissue birefringence from phase-sensitive measurements of orthogonal polarisation components of backscattering. In material science, polarisation has been used to study stress distribution, including the birefringence induced by stress in an otherwise isotropic material. Similar effects in biological tissues have not been well studied yet; however, may have application to tissues subjected to stress, e.g., tendons, muscles, lens, cornea or airway smooth muscle (ASM). The objective of this work is to explore stress-induced birefringence in tissue. We employ an advanced swept source-based PS-OCT system capable of measurement of tissue local polarisation properties. The sample in both cases is illuminated with orthogonal, passively depth-encoded polarisation states. Light returning from the tissue is detected via a polarisation-diversity detection module and a Mueller formalism is used to reconstruct polarisation properties (including retardation, diattenuation, and depolarisation) of the tissue. In this study, we demonstrate the measurement of stress-induced birefringence in phantoms and in soft tissues with polarisation sensitive optical coherence tomography.

Ultrahigh-Speed Optical Coherence Tomography for Three-Dimensional and En Face Imaging of the Retina and Optic Nerve Head

PubMed Central

Srinivasan, Vivek J.; Adler, Desmond C.; Chen, Yueli; Gorczynska, Iwona; Huber, Robert; Duker, Jay S.; Schuman, Joel S.; Fujimoto, James G.

2009-01-01

Purpose To demonstrate ultrahigh-speed optical coherence tomography (OCT) imaging of the retina and optic nerve head at 249,000 axial scans per second and a wavelength of 1060 nm. To investigate methods for visualization of the retina, choroid, and optic nerve using high-density sampling enabled by improved imaging speed. Methods A swept-source OCT retinal imaging system operating at a speed of 249,000 axial scans per second was developed. Imaging of the retina, choroid, and optic nerve were performed. Display methods such as speckle reduction, slicing along arbitrary planes, en face visualization of reflectance from specific retinal layers, and image compounding were investigated. Results High-definition and three-dimensional (3D) imaging of the normal retina and optic nerve head were performed. Increased light penetration at 1060 nm enabled improved visualization of the choroid, lamina cribrosa, and sclera. OCT fundus images and 3D visualizations were generated with higher pixel density and less motion artifacts than standard spectral/Fourier domain OCT. En face images enabled visualization of the porous structure of the lamina cribrosa, nerve fiber layer, choroid, photoreceptors, RPE, and capillaries of the inner retina. Conclusions Ultrahigh-speed OCT imaging of the retina and optic nerve head at 249,000 axial scans per second is possible. The improvement of ∼5 to 10× in imaging speed over commercial spectral/Fourier domain OCT technology enables higher density raster scan protocols and improved performance of en face visualization methods. The combination of the longer wavelength and ultrahigh imaging speed enables excellent visualization of the choroid, sclera, and lamina cribrosa. PMID:18658089

Detection and characterization of early plaque formations by Raman probe spectroscopy and optical coherence tomography: an in vivo study on a rabbit model

NASA Astrophysics Data System (ADS)

Matthäus, Christian; Dochow, Sebastian; Egodage, Kokila D.; Romeike, Bernd F.; Brehm, Bernhard R.; Popp, Jürgen

2018-01-01

Intravascular imaging techniques provide detailed specification about plaque appearance and morphology, but cannot deliver information about the biochemical composition of atherosclerotic plaques. As the biochemical composition is related to the plaque type, important aspects such as the risk of a plaque rupture and treatment are still difficult to assess. Currently, various spectroscopic techniques are tested for potential applications for the chemical analysis of plaque depositions. Here, we employ Raman spectroscopy in combination with optical coherence tomography (OCT) for the characterization of plaques on rabbits in vivo. Experiments were carried out on New Zealand white rabbits treated with a fat- and cholesterol-enriched diet, using a Raman probe setup with a 785-nm multimode laser as an excitation source. Subsequently, OCT images were acquired with a swept source at 1305±55 nm at 22.6 mW. Raman spectra were recorded from normal regions and regions with early plaque formations. The probe positioning was monitored by x-ray angiography. The spectral information identified plaque depositions consisting of lipids, with triglycerides as the major component. Afterward, OCT images of the spectroscopically investigated areas were obtained. The spectral information correlates well with the observed intravascular morphology and is in good agreement with histology. Raman spectroscopy can provide detailed biochemical specification of atherosclerotic plaques.

Challenges and advantages in wide-field optical coherence tomography angiography imaging of the human retinal and choroidal vasculature at 1.7-MHz A-scan rate

NASA Astrophysics Data System (ADS)

Poddar, Raju; Migacz, Justin V.; Schwartz, Daniel M.; Werner, John S.; Gorczynska, Iwona

2017-10-01

We present noninvasive, three-dimensional, depth-resolved imaging of human retinal and choroidal blood circulation with a swept-source optical coherence tomography (OCT) system at 1065-nm center wavelength. Motion contrast OCT imaging was performed with the phase-variance OCT angiography method. A Fourier-domain mode-locked light source was used to enable an imaging rate of 1.7 MHz. We experimentally demonstrate the challenges and advantages of wide-field OCT angiography (OCTA). In the discussion, we consider acquisition time, scanning area, scanning density, and their influence on visualization of selected features of the retinal and choroidal vascular networks. The OCTA imaging was performed with a field of view of 16 deg (5 mm×5 mm) and 30 deg (9 mm×9 mm). Data were presented in en face projections generated from single volumes and in en face projection mosaics generated from up to 4 datasets. OCTA imaging at 1.7 MHz A-scan rate was compared with results obtained from a commercial OCTA instrument and with conventional ophthalmic diagnostic methods: fundus photography, fluorescein, and indocyanine green angiography. Comparison of images obtained from all methods is demonstrated using the same eye of a healthy volunteer. For example, imaging of retinal pathology is presented in three cases of advanced age-related macular degeneration.

Fan Noise Source Diagnostic Test: Vane Unsteady Pressure Results

NASA Technical Reports Server (NTRS)

Envia, Edmane

2002-01-01

To investigate the nature of fan outlet guide vane pressure fluctuations and their link to rotor-stator interaction noise, time histories of vane fluctuating pressures were digitally acquired as part of the Fan Noise Source Diagnostic Test. Vane unsteady pressures were measured at seven fan tip speeds for both a radial and a swept vane configuration. Using time-domain averaging and spectral analysis, the blade passing frequency (BPF) harmonic and broadband contents of the vane pressures were individually analyzed. Significant Sound Pressure Level (SPL) reductions were observed for the swept vane relative to the radial vane for the BPF harmonics of vane pressure, but vane broadband reductions due to sweep turned out to be much smaller especially on an average basis. Cross-correlation analysis was used to establish the level of spatial coherence of broadband pressures between different locations on the vane and integral length scales of pressure fluctuations were estimated from these correlations. Two main results of this work are: (1) the average broadband level on the vane (in dB) increases linearly with the fan tip speed for both the radial and swept vanes, and (2) the broadband pressure distribution on the vane is nearly homogeneous and its integral length scale is a monotonically decreasing function of fan tip speed.

Pulsed electromagnetic gas acceleration. [magnetohydrodynamics, plasma power sources and plasma propulsion

NASA Technical Reports Server (NTRS)

Jahn, R. G.; Vonjaskowsky, W. F.; Clark, K. E.

1975-01-01

Terminal voltage measurements with various cathodes and anodes in a high power, quasi-steady magnetoplasmadynamic (MPD) are discussed. The magnitude of the current at the onset of voltage fluctuations is shown to be an increasing function of cathode area and a weaker decreasing function of anode area. Tests with a fluted cathode indicated that the fluctuations originate in the plasma adjacent to the cathode rather than at the cathode surface. Measurements of radiative output from an optical cavity aligned to examine the current-carrying portion of a two-dimensional, 56 kA magnetoplasmadynamic discharge reveal no lasing in that region, consistent with calculations of electron excitation and resonance radiation trapping. A voltage-swept double probe technique allows single-shot determination of electron temperature and electron number density in the recombining MPD exhaust flow. Current distributions within the cavity of MPD hollow cathodes for various static prefills with no injected mass flow are examined.

Towards the use of OCT angiography in clinical dermatology

NASA Astrophysics Data System (ADS)

Baran, Utku; Choi, Woo June; Wang, Ruikang K.

2016-02-01

Optical coherence tomography (OCT) is a popular imaging technique used in ophthalmology, and on the way to become clinically viable alternative in dermatology due to its capability of acquiring histopathology level images of in vivo tissue, noninvasively. In this study, we demonstrate the capabilities of OCT-based angiography (OMAG) in detecting high-resolution, volumetric structural and microvascular features of in vivo human skin with various conditions using a swept source OCT system that operates on a central wavelength of 1310 nm with an A-line rate of 100 kHz. OMAG images provide detailed in vivo visualization of microvasculature of abnormal human skin conditions from face, chest and belly. Moreover, the progress of wound healing on human skin from arm is monitored during longitudinal wound healing process. The presented results promise the clinical use of OCT angiography in treatment of prevalent cutaneous diseases within human skin, in vivo.

Closed loop tracked Doppler optical coherence tomography based heart monitor for the Drosophila melanogaster larvae.

PubMed

Zurauskas, Mantas; Bradu, Adrian; Ferguson, Daniel R; Hammer, Daniel X; Podoleanu, Adrian

2016-03-01

This paper presents a novel instrument for biosciences, useful for studies of moving embryos. A dual sequential imaging/measurement channel is assembled via a closed-loop tracking architecture. The dual channel system can operate in two regimes: (i) single-point Doppler signal monitoring or (ii) fast 3-D swept source OCT imaging. The system is demonstrated for characterizing cardiac dynamics in Drosophila melanogaster larva. Closed loop tracking enables long term in vivo monitoring of the larvae heart without anesthetic or physical restraint. Such an instrument can be used to measure subtle variations in the cardiac behavior otherwise obscured by the larvae movements. A fruit fly larva (top) was continuously tracked for continuous remote monitoring. A heartbeat trace of freely moving larva (bottom) was obtained by a low coherence interferometry based doppler sensing technique. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Human cervical carcinoma detection and glucose monitoring in blood micro vasculatures with swept source OCT

NASA Astrophysics Data System (ADS)

Ullah, H.; Ahmed, E.; Ikram, M.

2013-08-01

We report a pilot method, i.e., speckle variance (SV) and structured optical coherence tomography to visualize normal and malignant blood microvasculature in three and two dimensions and to monitor the glucose levels in blood by analyzing the Brownian motion of the red blood cells. The technique was applied on nude live mouse's skin and the obtained images depict the enhanced intravasculature network forum up to the depth of ˜2 mm with axial resolution of ˜8 μm. Microscopic images have also been obtained for both types of blood vessels to observe the tumor spatially. Our SV-OCT methodologies and results give satisfactory techniques in real time imaging and can potentially be applied during therapeutic techniques such as photodynamic therapy as well as to quantify the higher glucose levels injected intravenously to animal by determining the translation diffusion coefficient.

A Case of Bilateral Pigment Dispersion Syndrome Following Many Years of Uninterrupted Treatment With Atropine 1% for Bilateral Congenital Cataracts.

PubMed

Gizzi, Corrado; Mohamed-Noriega, Jibran; Murdoch, Ian

2017-10-01

Describe an unusual case of bilateral pigment dispersion syndrome (PDS) following years of uninterrupted treatment with atropine 1% for bilateral congenital cataracts, speculate on potential mechanisms leading to this condition. This is a case report. A 45-year-old white patient on long-term treatment with atropine 1% ointment since his infancy for bilateral congenital cataracts developed PDS with secondary ocular hypertension. The patient showed all the hallmarks of PDS with secondary ocular hypertension. An anterior segment Swept-Source optical coherence tomography was obtained to review the iris profile. The patient showed good pressure response to topical prostaglandin therapy. This is the second case report of PDS in a patient with chronic use of topical atropine. The proposed mechanisms for pigment dispersion are discussed and the possibility raised of dispersion being a potential side effect of the drug.

Large dynamic range optical vector analyzer based on optical single-sideband modulation and Hilbert transform

NASA Astrophysics Data System (ADS)

Xue, Min; Pan, Shilong; Zhao, Yongjiu

2016-07-01

A large dynamic range optical vector analyzer (OVA) based on optical single-sideband modulation is proposed and demonstrated. By dividing the optical signal after optical device under test into two paths, reversing the phase of one swept sideband using a Hilbert transformer in one path, and detecting the two signals from the two paths with a balanced photodetector, the measurement errors induced by the residual -1st-order sideband and the high-order sidebands can be eliminated and the dynamic range of the measurement is increased. In a proof-of-concept experiment, the stimulated Brillouin scattering and a fiber Bragg grating are measured by OVAs with and without the Hilbert transform and balanced photodetection. Results show that about 40-dB improvement in the measurement dynamic range is realized by the proposed OVA.

An experimental study of fluctuating pressure loads beneath swept shock/boundary-layer interactions

NASA Technical Reports Server (NTRS)

Settles, Gary S.

1991-01-01

A database is established on the fluctuating pressure loads produced on aerodynamic surfaces beneath 3-D shock wave/boundary layer interactions. Such loads constitute a fundamental problem of critical concern to future supersonic and hypersonic flight vehicles. A turbulent boundary layer on a flat plate is subjected to interactions with swept planar shock waves generated by sharp fins. Fin angles from 5 to 25 deg at freestream Mach numbers between 2.5 and 4 produce a variety of interaction strengths from weak to very strong. Miniature Kulite pressure transducers mounted in the flat plate were used to measure interaction-induced wall pressure fluctuations. These data will be correlated with proposed new optical data on the fluctuations of the interaction structure, especially that of the lambda-shock system and its associated high-speed jet impingement.

A versatile and modular quasi optics-based 200 GHz dual dynamic nuclear polarization and electron paramagnetic resonance instrument

PubMed Central

Siaw, Ting Ann; Leavesley, Alisa; Lund, Alicia; Kaminker, Ilia; Han, Songi

2016-01-01

Solid-state dynamic nuclear polarization (DNP) at higher magnetic fields (>3 T) and cryogenic temperatures (~2–90 K) has gained enormous interest and seen major technological advances as an NMR signal enhancing technique. Still, the current state of the art DNP operation is not at a state at which sample and freezing conditions can be rationally chosen and the DNP performance predicted a priori, but relies on purely empirical approaches. An important step towards rational optimization of DNP conditions is to have access to DNP instrumental capabilities to diagnose DNP performance and elucidate DNP mechanisms. The desired diagnoses include the measurement of the “DNP power curve”, i.e. the microwave (MW) power dependence of DNP enhancement, the “DNP spectrum”, i.e. the MW frequency dependence of DNP enhancement, the electron paramagnetic resonance (EPR) spectrum and the saturation and spectral diffusion properties of the EPR spectrum upon prolonged MW irradiation typical of continuous wave (CW) DNP, as well as various electron and nuclear spin relaxation parameters. Even basic measurements of these DNP parameters require versatile instrumentation at high magnetic fields not commercially available to date. In this article, we describe the detailed design of such a DNP instrument, powered by a solid-state MW source that is tunable between 193 – 201 GHz and outputs up to 140 mW of MW power. The quality and pathway of the transmitted and reflected MWs is controlled by a quasi-optics (QO) bridge and a corrugated waveguide, where the latter couples the MW from an open-space QO bridge to the sample located inside the superconducting magnet and vice versa. Crucially, the versatility of the solid-state MW source enables the automated acquisition of frequency swept DNP spectra, DNP power curves, the diagnosis of MW power and transmission, and frequency swept continuous wave (CW) and pulsed EPR experiments. The flexibility of the DNP instrument centered around the QO MW bridge will provide an efficient means to collect DNP data that is crucial for understanding the relationship between experimental and sample conditions, and the DNP performance. The modularity of this instrumental platform is suitable for future upgrades and extensions to include new experimental capabilities to meet contemporary DNP needs, including the simultaneous operation of two or more MW sources, time domain DNP, electron double resonance measurements, pulsed EPR operation, or simply the implementation of higher power MW amplifiers. PMID:26920839

A versatile and modular quasi optics-based 200 GHz dual dynamic nuclear polarization and electron paramagnetic resonance instrument

NASA Astrophysics Data System (ADS)

Siaw, Ting Ann; Leavesley, Alisa; Lund, Alicia; Kaminker, Ilia; Han, Songi

2016-03-01

Solid-state dynamic nuclear polarization (DNP) at higher magnetic fields (>3 T) and cryogenic temperatures (∼2-90 K) has gained enormous interest and seen major technological advances as an NMR signal enhancing technique. Still, the current state of the art DNP operation is not at a state at which sample and freezing conditions can be rationally chosen and the DNP performance predicted a priori, but relies on purely empirical approaches. An important step towards rational optimization of DNP conditions is to have access to DNP instrumental capabilities to diagnose DNP performance and elucidate DNP mechanisms. The desired diagnoses include the measurement of the "DNP power curve", i.e. the microwave (MW) power dependence of DNP enhancement, the "DNP spectrum", i.e. the MW frequency dependence of DNP enhancement, the electron paramagnetic resonance (EPR) spectrum, and the saturation and spectral diffusion properties of the EPR spectrum upon prolonged MW irradiation typical of continuous wave (CW) DNP, as well as various electron and nuclear spin relaxation parameters. Even basic measurements of these DNP parameters require versatile instrumentation at high magnetic fields not commercially available to date. In this article, we describe the detailed design of such a DNP instrument, powered by a solid-state MW source that is tunable between 193 and 201 GHz and outputs up to 140 mW of MW power. The quality and pathway of the transmitted and reflected MWs is controlled by a quasi-optics (QO) bridge and a corrugated waveguide, where the latter couples the MW from an open-space QO bridge to the sample located inside the superconducting magnet and vice versa. Crucially, the versatility of the solid-state MW source enables the automated acquisition of frequency swept DNP spectra, DNP power curves, the diagnosis of MW power and transmission, and frequency swept continuous wave (CW) and pulsed EPR experiments. The flexibility of the DNP instrument centered around the QO MW bridge will provide an efficient means to collect DNP data that is crucial for understanding the relationship between experimental and sample conditions, and the DNP performance. The modularity of this instrumental platform is suitable for future upgrades and extensions to include new experimental capabilities to meet contemporary DNP needs, including the simultaneous operation of two or more MW sources, time domain DNP, electron double resonance measurements, pulsed EPR operation, or simply the implementation of higher power MW amplifiers.

A versatile and modular quasi optics-based 200GHz dual dynamic nuclear polarization and electron paramagnetic resonance instrument.

PubMed

Siaw, Ting Ann; Leavesley, Alisa; Lund, Alicia; Kaminker, Ilia; Han, Songi

2016-03-01

Solid-state dynamic nuclear polarization (DNP) at higher magnetic fields (>3T) and cryogenic temperatures (∼ 2-90K) has gained enormous interest and seen major technological advances as an NMR signal enhancing technique. Still, the current state of the art DNP operation is not at a state at which sample and freezing conditions can be rationally chosen and the DNP performance predicted a priori, but relies on purely empirical approaches. An important step towards rational optimization of DNP conditions is to have access to DNP instrumental capabilities to diagnose DNP performance and elucidate DNP mechanisms. The desired diagnoses include the measurement of the "DNP power curve", i.e. the microwave (MW) power dependence of DNP enhancement, the "DNP spectrum", i.e. the MW frequency dependence of DNP enhancement, the electron paramagnetic resonance (EPR) spectrum, and the saturation and spectral diffusion properties of the EPR spectrum upon prolonged MW irradiation typical of continuous wave (CW) DNP, as well as various electron and nuclear spin relaxation parameters. Even basic measurements of these DNP parameters require versatile instrumentation at high magnetic fields not commercially available to date. In this article, we describe the detailed design of such a DNP instrument, powered by a solid-state MW source that is tunable between 193 and 201 GHz and outputs up to 140 mW of MW power. The quality and pathway of the transmitted and reflected MWs is controlled by a quasi-optics (QO) bridge and a corrugated waveguide, where the latter couples the MW from an open-space QO bridge to the sample located inside the superconducting magnet and vice versa. Crucially, the versatility of the solid-state MW source enables the automated acquisition of frequency swept DNP spectra, DNP power curves, the diagnosis of MW power and transmission, and frequency swept continuous wave (CW) and pulsed EPR experiments. The flexibility of the DNP instrument centered around the QO MW bridge will provide an efficient means to collect DNP data that is crucial for understanding the relationship between experimental and sample conditions, and the DNP performance. The modularity of this instrumental platform is suitable for future upgrades and extensions to include new experimental capabilities to meet contemporary DNP needs, including the simultaneous operation of two or more MW sources, time domain DNP, electron double resonance measurements, pulsed EPR operation, or simply the implementation of higher power MW amplifiers. Copyright © 2015 Elsevier Inc. All rights reserved.

Sequential, progressive, equal-power, reflective beam-splitter arrays

NASA Astrophysics Data System (ADS)

Manhart, Paul K.

2017-11-01

The equations to calculate equal-power reflectivity of a sequential series of beam splitters is presented. Non-sequential optical design examples are offered for uniform illumination using diode lasers. Objects created using Boolean operators and Swept Surfaces can create objects capable of reflecting light into predefined elevation and azimuth angles. Analysis of the illumination patterns for the array are also presented.

Combined multimodal photoacoustic tomography, optical coherence tomography (OCT) and OCT based angiography system for in vivo imaging of multiple skin disorders in human(Conference Presentation)

NASA Astrophysics Data System (ADS)

Liu, Mengyang; Chen, Zhe; Sinz, Christoph; Rank, Elisabet; Zabihian, Behrooz; Zhang, Edward Z.; Beard, Paul C.; Kittler, Harald; Drexler, Wolfgang

2017-02-01

All optical photoacoustic tomography (PAT) using a planar Fabry-Perot interferometer polymer film sensor has been demonstrated for in vivo human palm imaging with an imaging penetration depth of 5 mm. The relatively larger vessels in the superficial plexus and the vessels in the dermal plexus are visible in PAT. However, due to both resolution and sensitivity limits, all optical PAT cannot reveal the smaller vessels such as capillary loops and venules. Melanin absorption also sometimes causes difficulties in PAT to resolve vessels. Optical coherence tomography (OCT) based angiography, on the other hand, has been proven suitable for microvasculature visualization in the first couple millimeters in human. In our work, we combine an all optical PAT system with an OCT system featuring a phase stable akinetic swept source. This multimodal PAT/OCT/OCT-angiography system provides us co-registered human skin vasculature information as well as the structural information of cutaneous. The scanning units of the sub-systems are assembled into one probe, which is then mounted onto a portable rack. The probe and rack design gives six degrees of freedom, allowing the multimodal optical imaging probe to access nearly all regions of human body. Utilizing this probe, we perform imaging on patients with various skin disorders as well as on healthy controls. Fused PAT/OCT-angiography volume shows the complete blood vessel network in human skin, which is further embedded in the morphology provided by OCT. A comparison between the results from the disordered regions and the normal regions demonstrates the clinical translational value of this multimodal optical imaging system in dermatology.

Swept-frequency feedback interferometry using terahertz frequency QCLs: a method for imaging and materials analysis.

PubMed

Rakić, Aleksandar D; Taimre, Thomas; Bertling, Karl; Lim, Yah Leng; Dean, Paul; Indjin, Dragan; Ikonić, Zoran; Harrison, Paul; Valavanis, Alexander; Khanna, Suraj P; Lachab, Mohammad; Wilson, Stephen J; Linfield, Edmund H; Davies, A Giles

2013-09-23

The terahertz (THz) frequency quantum cascade laser (QCL) is a compact source of high-power radiation with a narrow intrinsic linewidth. As such, THz QCLs are extremely promising sources for applications including high-resolution spectroscopy, heterodyne detection, and coherent imaging. We exploit the remarkable phase-stability of THz QCLs to create a coherent swept-frequency delayed self-homodyning method for both imaging and materials analysis, using laser feedback interferometry. Using our scheme we obtain amplitude-like and phase-like images with minimal signal processing. We determine the physical relationship between the operating parameters of the laser under feedback and the complex refractive index of the target and demonstrate that this coherent detection method enables extraction of complex refractive indices with high accuracy. This establishes an ultimately compact and easy-to-implement THz imaging and materials analysis system, in which the local oscillator, mixer, and detector are all combined into a single laser.

Visual perception enhancement for detection of cancerous oral tissue by multi-spectral imaging

NASA Astrophysics Data System (ADS)

Wang, Hsiang-Chen; Tsai, Meng-Tsan; Chiang, Chun-Ping

2013-05-01

Color reproduction systems based on the multi-spectral imaging technique (MSI) for both directly estimating reflection spectra and direct visualization of oral tissues using various light sources are proposed. Images from three oral cancer patients were taken as the experimental samples, and spectral differences between pre-cancerous and normal oral mucosal tissues were calculated at three time points during 5-aminolevulinic acid photodynamic therapy (ALA-PDT) to analyze whether they were consistent with disease processes. To check the successful treatment of oral cancer with ALA-PDT, oral cavity images by swept source optical coherence tomography (SS-OCT) are demonstrated. This system can also reproduce images under different light sources. For pre-cancerous detection, the oral images after the second ALA-PDT are assigned as the target samples. By using RGB LEDs with various correlated color temperatures (CCTs) for color difference comparison, the light source with a CCT of about 4500 K was found to have the best ability to enhance the color difference between pre-cancerous and normal oral mucosal tissues in the oral cavity. Compared with the fluorescent lighting commonly used today, the color difference can be improved by 39.2% from 16.5270 to 23.0023. Hence, this light source and spectral analysis increase the efficiency of the medical diagnosis of oral cancer and aid patients in receiving early treatment.

Necessity of angiotensin-converting enzyme-related gene for cardiac functions and longevity of Drosophila melanogaster assessed by optical coherence tomography

NASA Astrophysics Data System (ADS)

Liao, Fang-Tsu; Chang, Cheng-Yi; Su, Ming-Tsan; Kuo, Wen-Chuan

2014-01-01

Prior studies have established the necessity of an angiotensin-converting enzyme-related (ACER) gene for heart morphogenesis of Drosophila. Nevertheless, the physiology of ACER has yet to be comprehensively understood. Herein, we employed RNA interference to down-regulate the expression of ACER in Drosophila's heart and swept source optical coherence tomography to assess whether ACER is required for cardiac functions in living adult flies. Several contractile parameters of Drosophila heart, including the heart rate (HR), end-diastolic diameter (EDD), end-systolic diameter (ESD), percent fractional shortening (%FS), and stress-induced cardiac performance, are shown, which are age dependent. These age-dependent cardiac functions declined significantly when ACER was down-regulated. Moreover, the lifespans of ACER knock-down flies were significantly shorter than those of wild-type control flies. Thus, we posit that ACER, the Drosophila ortholog of mammalian angiotensin-converting enzyme 2 (ACE2), is essential for both heart physiology and longevity of animals. Since mammalian ACE2 controls many cardiovascular physiological features and is implicated in cardiomyopathies, our findings that ACER plays conserved roles in genetically tractable animals will pave the way for uncovering the genetic pathway that controls the renin-angiotensin system.

Ultrahigh speed endoscopic optical coherence tomography for gastroenterology.

PubMed

Tsai, Tsung-Han; Lee, Hsiang-Chieh; Ahsen, Osman O; Liang, Kaicheng; Giacomelli, Michael G; Potsaid, Benjamin M; Tao, Yuankai K; Jayaraman, Vijaysekhar; Figueiredo, Marisa; Huang, Qin; Cable, Alex E; Fujimoto, James; Mashimo, Hiroshi

2014-12-01

We describe an ultrahigh speed endoscopic swept source optical coherence tomography (OCT) system for clinical gastroenterology using a vertical-cavity surface-emitting laser (VCSEL) and micromotor imaging catheter. The system had a 600 kHz axial scan rate and 8 µm axial resolution in tissue. Imaging was performed with a 3.2 mm diameter imaging catheter at 400 frames per second with a 12 µm spot size. Three-dimensional OCT (3D-OCT) imaging was performed in patients with a cross section of pathologies undergoing upper and lower endoscopy. The use of distally actuated imaging catheters enabled OCT imaging with more flexibility, such as volumetric imaging in the small intestine and the assessment of hiatal hernia using retroflex imaging. The high rotational scanning stability of the micromotor enabled 3D volumetric imaging with micron scale volumetric accuracy for both en face OCT and cross-sectional imaging, as well as OCT angiography (OCTA) for 3D visualization of subsurface microvasculature. The ability to perform both structural and functional 3D OCT imaging in the GI tract with microscopic accuracy should enable a wide range of studies and enhance the sensitivity and specificity of OCT for detecting pathology.

Ultrahigh speed endoscopic optical coherence tomography for gastroenterology

PubMed Central

Tsai, Tsung-Han; Lee, Hsiang-Chieh; Ahsen, Osman O.; Liang, Kaicheng; Giacomelli, Michael G.; Potsaid, Benjamin M.; Tao, Yuankai K.; Jayaraman, Vijaysekhar; Figueiredo, Marisa; Huang, Qin; Cable, Alex E.; Fujimoto, James; Mashimo, Hiroshi

2014-01-01

We describe an ultrahigh speed endoscopic swept source optical coherence tomography (OCT) system for clinical gastroenterology using a vertical-cavity surface-emitting laser (VCSEL) and micromotor imaging catheter. The system had a 600 kHz axial scan rate and 8 µm axial resolution in tissue. Imaging was performed with a 3.2 mm diameter imaging catheter at 400 frames per second with a 12 µm spot size. Three-dimensional OCT (3D-OCT) imaging was performed in patients with a cross section of pathologies undergoing upper and lower endoscopy. The use of distally actuated imaging catheters enabled OCT imaging with more flexibility, such as volumetric imaging in the small intestine and the assessment of hiatal hernia using retroflex imaging. The high rotational scanning stability of the micromotor enabled 3D volumetric imaging with micron scale volumetric accuracy for both en face OCT and cross-sectional imaging, as well as OCT angiography (OCTA) for 3D visualization of subsurface microvasculature. The ability to perform both structural and functional 3D OCT imaging in the GI tract with microscopic accuracy should enable a wide range of studies and enhance the sensitivity and specificity of OCT for detecting pathology. PMID:25574446

Circumferential optical coherence tomography angiography imaging of the swine esophagus using a micromotor balloon catheter.

PubMed

Lee, Hsiang-Chieh; Ahsen, Osman Oguz; Liang, Kaicheng; Wang, Zhao; Cleveland, Cody; Booth, Lucas; Potsaid, Benjamin; Jayaraman, Vijaysekhar; Cable, Alex E; Mashimo, Hiroshi; Langer, Robert; Traverso, Giovanni; Fujimoto, James G

2016-08-01

We demonstrate a micromotor balloon imaging catheter for ultrahigh speed endoscopic optical coherence tomography (OCT) which provides wide area, circumferential structural and angiographic imaging of the esophagus without contrast agents. Using a 1310 nm MEMS tunable wavelength swept VCSEL light source, the system has a 1.2 MHz A-scan rate and ~8.5 µm axial resolution in tissue. The micromotor balloon catheter enables circumferential imaging of the esophagus at 240 frames per second (fps) with a ~30 µm (FWHM) spot size. Volumetric imaging is achieved by proximal pullback of the micromotor assembly within the balloon at 1.5 mm/sec. Volumetric data consisting of 4200 circumferential images of 5,000 A-scans each over a 2.6 cm length, covering a ~13 cm(2) area is acquired in <18 seconds. A non-rigid image registration algorithm is used to suppress motion artifacts from non-uniform rotational distortion (NURD), cardiac motion or respiration. En face OCT images at various depths can be generated. OCT angiography (OCTA) is computed using intensity decorrelation between sequential pairs of circumferential scans and enables three-dimensional visualization of vasculature. Wide area volumetric OCT and OCTA imaging of the swine esophagus in vivo is demonstrated.

3D optical coherence tomography image registration for guiding cochlear implant insertion

NASA Astrophysics Data System (ADS)

Cheon, Gyeong-Woo; Jeong, Hyun-Woo; Chalasani, Preetham; Chien, Wade W.; Iordachita, Iulian; Taylor, Russell; Niparko, John; Kang, Jin U.

2014-03-01

In cochlear implant surgery, an electrode array is inserted into the cochlear canal to restore hearing to a person who is profoundly deaf or significantly hearing impaired. One critical part of the procedure is the insertion of the electrode array, which looks like a thin wire, into the cochlear canal. Although X-ray or computed tomography (CT) could be used as a reference to evaluate the pathway of the whole electrode array, there is no way to depict the intra-cochlear canal and basal turn intra-operatively to help guide insertion of the electrode array. Optical coherent tomography (OCT) is a highly effective way of visualizing internal structures of cochlea. Swept source OCT (SSOCT) having center wavelength of 1.3 micron and 2D Galvonometer mirrors was used to achieve 7-mm depth 3-D imaging. Graphics processing unit (GPU), OpenGL, C++ and C# were integrated for real-time volumetric rendering simultaneously. The 3D volume images taken by the OCT system were assembled and registered which could be used to guide a cochlear implant. We performed a feasibility study using both dry and wet temporal bones and the result is presented.

Multispectral analog-mean-delay fluorescence lifetime imaging combined with optical coherence tomography

PubMed Central

Nam, Hyeong Soo; Kang, Woo Jae; Lee, Min Woo; Song, Joon Woo; Kim, Jin Won; Oh, Wang-Yuhl; Yoo, Hongki

2018-01-01

The pathophysiological progression of chronic diseases, including atherosclerosis and cancer, is closely related to compositional changes in biological tissues containing endogenous fluorophores such as collagen, elastin, and NADH, which exhibit strong autofluorescence under ultraviolet excitation. Fluorescence lifetime imaging (FLIm) provides robust detection of the compositional changes by measuring fluorescence lifetime, which is an inherent property of a fluorophore. In this paper, we present a dual-modality system combining a multispectral analog-mean-delay (AMD) FLIm and a high-speed swept-source optical coherence tomography (OCT) to simultaneously visualize the cross-sectional morphology and biochemical compositional information of a biological tissue. Experiments using standard fluorescent solutions showed that the fluorescence lifetime could be measured with a precision of less than 40 psec using the multispectral AMD-FLIm without averaging. In addition, we performed ex vivo imaging on rabbit iliac normal-looking and atherosclerotic specimens to demonstrate the feasibility of the combined FLIm-OCT system for atherosclerosis imaging. We expect that the combined FLIm-OCT will be a promising next-generation imaging technique for diagnosing atherosclerosis and cancer due to the advantages of the proposed label-free high-precision multispectral lifetime measurement. PMID:29675330

Cycloid scanning for wide field optical coherence tomography endomicroscopy and angiography in vivo

PubMed Central

Liang, Kaicheng; Wang, Zhao; Ahsen, Osman O.; Lee, Hsiang-Chieh; Potsaid, Benjamin M.; Jayaraman, Vijaysekhar; Cable, Alex; Mashimo, Hiroshi; Li, Xingde; Fujimoto, James G.

2018-01-01

Devices that perform wide field-of-view (FOV) precision optical scanning are important for endoscopic assessment and diagnosis of luminal organ disease such as in gastroenterology. Optical scanning for in vivo endoscopic imaging has traditionally relied on one or more proximal mechanical actuators, limiting scan accuracy and imaging speed. There is a need for rapid and precise two-dimensional (2D) microscanning technologies to enable the translation of benchtop scanning microscopies to in vivo endoscopic imaging. We demonstrate a new cycloid scanner in a tethered capsule for ultrahigh speed, side-viewing optical coherence tomography (OCT) endomicroscopy in vivo. The cycloid capsule incorporates two scanners: a piezoelectrically actuated resonant fiber scanner to perform a precision, small FOV, fast scan and a micromotor scanner to perform a wide FOV, slow scan. Together these scanners distally scan the beam circumferentially in a 2D cycloid pattern, generating an unwrapped 1 mm × 38 mm strip FOV. Sequential strip volumes can be acquired with proximal pullback to image centimeter-long regions. Using ultrahigh speed 1.3 μm wavelength swept-source OCT at a 1.17 MHz axial scan rate, we imaged the human rectum at 3 volumes/s. Each OCT strip volume had 166 × 2322 axial scans with 8.5 μm axial and 30 μm transverse resolution. We further demonstrate OCT angiography at 0.5 volumes/s, producing volumetric images of vasculature. In addition to OCT applications, cycloid scanning promises to enable precision 2D optical scanning for other imaging modalities, including fluorescence confocal and nonlinear microscopy. PMID:29682598

Resolution and throughput optimized intraoperative spectrally encoded coherence tomography and reflectometry (iSECTR) for multimodal imaging during ophthalmic microsurgery

NASA Astrophysics Data System (ADS)

Malone, Joseph D.; El-Haddad, Mohamed T.; Leeburg, Kelsey C.; Terrones, Benjamin D.; Tao, Yuankai K.

2018-02-01

Limited visualization of semi-transparent structures in the eye remains a critical barrier to improving clinical outcomes and developing novel surgical techniques. While increases in imaging speed has enabled intraoperative optical coherence tomography (iOCT) imaging of surgical dynamics, several critical barriers to clinical adoption remain. Specifically, these include (1) static field-of-views (FOVs) requiring manual instrument-tracking; (2) high frame-rates require sparse sampling, which limits FOV; and (3) small iOCT FOV also limits the ability to co-register data with surgical microscopy. We previously addressed these limitations in image-guided ophthalmic microsurgery by developing microscope-integrated multimodal intraoperative swept-source spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography. Complementary en face images enabled orientation and coregistration with the widefield surgical microscope view while OCT imaging enabled depth-resolved visualization of surgical instrument positions relative to anatomic structures-of-interest. In addition, we demonstrated novel integrated segmentation overlays for augmented-reality surgical guidance. Unfortunately, our previous system lacked the resolution and optical throughput for in vivo retinal imaging and necessitated removal of cornea and lens. These limitations were predominately a result of optical aberrations from imaging through a shared surgical microscope objective lens, which was modeled as a paraxial surface. Here, we present an optimized intraoperative spectrally encoded coherence tomography and reflectometry (iSECTR) system. We use a novel lens characterization method to develop an accurate model of surgical microscope objective performance and balance out inherent aberrations using iSECTR relay optics. Using this system, we demonstrate in vivo multimodal ophthalmic imaging through a surgical microscope

Swept shock/boundary-layer interactions: Scaling laws, flowfield structure, and experimental methods

NASA Technical Reports Server (NTRS)

Settles, Gary S.

1993-01-01

A general review is given of several decades of research on the scaling laws and flowfield structures of swept shock wave/turbulent boundary layer interactions. Attention is further restricted to the experimental study and physical understanding of the steady-state aspects of these flows. The interaction produced by a sharp, upright fin mounted on a flat plate is taken as an archetype. An overall framework of quasiconical symmetry describing such interactions is first developed. Boundary-layer separation, the interaction footprint, Mach number scaling, and Reynolds number scaling are then considered, followed by a discussion of the quasiconical similarity of interactions produced by geometrically-dissimilar shock generators. The detailed structure of these interaction flowfields is next reviewed, and is illustrated by both qualitative visualizations and quantitative flow images in the quasiconical framework. Finally, the experimental techniques used to investigate such flows are reviewed, with emphasis on modern non-intrusive optical flow diagnostics.

Combined 60° Wide-Field Choroidal Thickness Maps and High-Definition En Face Vasculature Visualization Using Swept-Source Megahertz OCT at 1050 nm

PubMed Central

Mohler, Kathrin J.; Draxinger, Wolfgang; Klein, Thomas; Kolb, Jan Philip; Wieser, Wolfgang; Haritoglou, Christos; Kampik, Anselm; Fujimoto, James G.; Neubauer, Aljoscha S.; Huber, Robert; Wolf, Armin

2015-01-01

Purpose To demonstrate ultrahigh-speed swept-source optical coherence tomography (SS-OCT) at 1.68 million A-scans/s for choroidal imaging in normal and diseased eyes over a ∼60° field of view. To investigate and correlate wide-field three-dimensional (3D) choroidal thickness (ChT) and vascular patterns using ChT maps and coregistered high-definition en face images extracted from a single densely sampled Megahertz-OCT (MHz-OCT) dataset. Methods High-definition, ∼60° wide-field 3D datasets consisting of 2088 × 1024 A-scans were acquired using a 1.68 MHz prototype SS-OCT system at 1050 nm based on a Fourier-domain mode-locked laser. Nine subjects (nine eyes) with various chorioretinal diseases or without ocular pathology are presented. Coregistered ChT maps, choroidal summation maps, and depth-resolved en face images referenced to either the retinal pigment epithelium or the choroidal–scleral interface were generated using manual segmentation. Results Wide-field ChT maps showed a large inter- and intraindividual variance in peripheral and central ChT. In only four of the nine eyes, the location with the largest ChT was coincident with the fovea. The anatomy of the large lumen vessels of the outer choroid seems to play a major role in determining the global ChT pattern. Focal ChT changes with large thickness gradients were observed in some eyes. Conclusions Different ChT and vascular patterns could be visualized over ∼60° in patients for the first time using OCT. Due to focal ChT changes, a high density of thickness measurements may be favorable. High-definition depth-resolved en face images are complementary to cross sections and thickness maps and enhance the interpretation of different ChT patterns. PMID:26431482

Combined 60° Wide-Field Choroidal Thickness Maps and High-Definition En Face Vasculature Visualization Using Swept-Source Megahertz OCT at 1050 nm.

PubMed

Mohler, Kathrin J; Draxinger, Wolfgang; Klein, Thomas; Kolb, Jan Philip; Wieser, Wolfgang; Haritoglou, Christos; Kampik, Anselm; Fujimoto, James G; Neubauer, Aljoscha S; Huber, Robert; Wolf, Armin

2015-10-01

To demonstrate ultrahigh-speed swept-source optical coherence tomography (SS-OCT) at 1.68 million A-scans/s for choroidal imaging in normal and diseased eyes over a ∼60° field of view. To investigate and correlate wide-field three-dimensional (3D) choroidal thickness (ChT) and vascular patterns using ChT maps and coregistered high-definition en face images extracted from a single densely sampled Megahertz-OCT (MHz-OCT) dataset. High-definition, ∼60° wide-field 3D datasets consisting of 2088 × 1024 A-scans were acquired using a 1.68 MHz prototype SS-OCT system at 1050 nm based on a Fourier-domain mode-locked laser. Nine subjects (nine eyes) with various chorioretinal diseases or without ocular pathology are presented. Coregistered ChT maps, choroidal summation maps, and depth-resolved en face images referenced to either the retinal pigment epithelium or the choroidal-scleral interface were generated using manual segmentation. Wide-field ChT maps showed a large inter- and intraindividual variance in peripheral and central ChT. In only four of the nine eyes, the location with the largest ChT was coincident with the fovea. The anatomy of the large lumen vessels of the outer choroid seems to play a major role in determining the global ChT pattern. Focal ChT changes with large thickness gradients were observed in some eyes. Different ChT and vascular patterns could be visualized over ∼60° in patients for the first time using OCT. Due to focal ChT changes, a high density of thickness measurements may be favorable. High-definition depth-resolved en face images are complementary to cross sections and thickness maps and enhance the interpretation of different ChT patterns.

High-speed polarization sensitive optical coherence tomography for retinal diagnostics

NASA Astrophysics Data System (ADS)

Yin, Biwei; Wang, Bingqing; Vemishetty, Kalyanramu; Nagle, Jim; Liu, Shuang; Wang, Tianyi; Rylander, Henry G., III; Milner, Thomas E.

2012-01-01

We report design and construction of an FPGA-based high-speed swept-source polarization-sensitive optical coherence tomography (SS-PS-OCT) system for clinical retinal imaging. Clinical application of the SS-PS-OCT system is accurate measurement and display of thickness, phase retardation and birefringence maps of the retinal nerve fiber layer (RNFL) in human subjects for early detection of glaucoma. The FPGA-based SS-PS-OCT system provides three incident polarization states on the eye and uses a bulk-optic polarization sensitive balanced detection module to record two orthogonal interference fringe signals. Interference fringe signals and relative phase retardation between two orthogonal polarization states are used to obtain Stokes vectors of light returning from each RNFL depth. We implement a Levenberg-Marquardt algorithm on a Field Programmable Gate Array (FPGA) to compute accurate phase retardation and birefringence maps. For each retinal scan, a three-state Levenberg-Marquardt nonlinear algorithm is applied to 360 clusters each consisting of 100 A-scans to determine accurate maps of phase retardation and birefringence in less than 1 second after patient measurement allowing real-time clinical imaging-a speedup of more than 300 times over previous implementations. We report application of the FPGA-based SS-PS-OCT system for real-time clinical imaging of patients enrolled in a clinical study at the Eye Institute of Austin and Duke Eye Center.

Resolution characteristics of optical coherence tomography for dental use.

PubMed

Watanabe, Hiroshi; Kuribayashi, Ami; Sumi, Yasunori; Kurabayashi, Tohru

2017-03-01

The purpose of this study was to clarify the resolution characteristics of optical coherence tomography (OCT) for dental use. Two types of swept-source optical coherence tomography machines were employed in this study. To clarify their resolution characteristics, we newly developed a glass chart device with a ladder pattern of wavelengths, which ranged from 4 × 2 μm to 1024 × 2 μm, as well as a star-target pattern, a grid pattern and a spatial frequency response pattern. The resolving powers and characteristics of the OCTs were subjectively evaluated. The Santec OCT-2000 ™ (Santec Co., Komaki, Japan) had a resolving power of 64 μm in both the horizontal X and vertical Y directions, while the OCT from Yoshida had a resolving power of 64 μm in the horizontal X direction and 128 µm in the vertical Y direction. The resolving power of the depth Z direction could not be obtained from this study. With the Yoshida OCT, the star-target pattern seemed to be non-symmetrical, owing to an edge enhancement effect, which was revealed when the ladder patterns were placed in a horizontal direction. This study successfully clarified the resolution characteristics of two types of OCTs. The obtained data may be useful for diagnostic purposes, and the glass chart device used in this study may be useful for OCT quality assurance programmes.

Evaluation of Optical Coherence Tomography for the Measurement of the Effects of Activators and Anticoagulants on the Blood Coagulation In Vitro

PubMed Central

Geng, Jinhai; Liu, Gangjun; Chen, Zhongping

2013-01-01

Optical properties of human blood during coagulation were studied using optical coherence tomography (OCT) and the parameter of clotting time derived from the 1/e light penetration depth (d1/e) versus time was developed in our previous work. In this study, in order to know if a new OCT test can characterize the blood-coagulation process under different treatments in vitro, the effects of two different activators (calcium ions and thrombin) and anticoagulants, i.e., acetylsalicylic acid (ASA, a well-known drug aspirin) and melagatran (a direct thrombin inhibitor), at various concentrations are evaluated. A swept-source OCT system with a 1300 nm center wavelength is used for detecting the blood-coagulation process in vitro under a static condition. A dynamic study of d1/e reveals a typical behavior due to coagulation induced by both calcium ions and thrombin, and the clotting time is concentration-dependent. Dose-dependent ASA and melagatran prolong the clotting times. ASA and melagatran have different effects on blood coagulation. As expected, melagatran is much more effective than ASA in anticoagulation by the OCT measurements. The OCT assay appears to be a simple method for the measurement of blood coagulation to assess the effects of activators and anticoagulants, which can be used for activator and anticoagulant screening. PMID:23392340

Evaluation of optical coherence tomography for the measurement of the effects of activators and anticoagulants on the blood coagulation in vitro.

PubMed

Xu, Xiangqun; Geng, Jinhai; Liu, Gangjun; Chen, Zhongping

2013-08-01

Optical properties of human blood during coagulation were studied using optical coherence tomography (OCT) and the parameter of clotting time derived from the 1/e light penetration depth (d(1/e)) versus time was developed in our previous work. In this study, in order to know if a new OCT test can characterize the blood-coagulation process under different treatments in vitro, the effects of two different activators (calcium ions and thrombin) and anticoagulants, i.e., acetylsalicylic acid (ASA, a well-known drug aspirin) and melagatran (a direct thrombin inhibitor), at various concentrations are evaluated. A swept-source OCT system with a 1300 nm center wavelength is used for detecting the blood-coagulation process in vitro under a static condition. A dynamic study of d1/e reveals a typical behavior due to coagulation induced by both calcium ions and thrombin, and the clotting time is concentration-dependent. Dose-dependent ASA and melagatran prolong the clotting times. ASA and melagatran have different effects on blood coagulation. As expected, melagatran is much more effective than ASA in anticoagulation by the OCT measurements. The OCT assay appears to be a simple method for the measurement of blood coagulation to assess the effects of activators and anticoagulants, which can be used for activator and anticoagulant screening.

Long working distance optical coherence tomography for pediatric imaging (Conference Presentation)

NASA Astrophysics Data System (ADS)

Qian, Ruobing; Carrasco-Zevallos, Oscar; Vajzovic, Lejla; Gramatikov, Boris I.; Guyton, David L.; Toth, Cynthia A.; Izatt, Joseph A.

2016-03-01

Conventional optical coherence tomography (OCT) systems have working distances of about 25 mm, and require cooperative subjects to immobilize and fixate on a target. Handheld OCT probes have also been demonstrated for successful imaging of pre-term infants and neonates up to ~1 year old. However, no technology yet exists for OCT in young children due to their lack of attention and inherent fear of large objects close to their face. In this work, we demonstrate a prototype retinal swept-source OCT system with a long working distance (from the last optical element to the subject's eye) to facilitate pediatric imaging. To reduce the footprint and weight of the system compared to the conventional 4f scheme, a novel 2f scanning configuration was implemented to achieve a working distance of 348mm with a +/- 8° scanning angle prior to cornea. Employing two custom-designed lenses, the system design resolution was nearly diffraction limited throughout a -8D to +5D refractive corrections. A fixation target displayed on a LCD monitor and an iris camera were used to facilitate alignment and imaging. Our prototype was tested in consented adult subjects and has the potential to facilitate imaging of young children. With this apparatus, young children could potentially sit comfortably in caretaker's lap while viewing entertainment on the fixation screen designed to direct their gaze into the imaging apparatus.

Noncontact phase-sensitive dynamic optical coherence elastography at megahertz rate

NASA Astrophysics Data System (ADS)

Singh, Manmohan; Wu, Chen; Liu, Chih-Hao; Li, Jiasong; Schill, Alexander; Nair, Achuth; Kistenev, Yury V.; Larin, Kirill V.

2016-03-01

Dynamic optical coherence elastography (OCE) techniques have shown great promise at quantitatively obtaining the biomechanical properties of tissue. However, the majority of these techniques have required multiple temporal OCT acquisitions (M-B mode) and corresponding excitations, which lead to clinically unfeasible acquisition times and potential tissue damage. Furthermore, the large data sets and extended laser exposures hinder their translation to the clinic, where patient discomfort and safety are critical criteria. In this work we demonstrate noncontact true kilohertz frame-rate dynamic optical coherence elastography by directly imaging a focused air-pulse induced elastic wave with a home-built phase-sensitive OCE system based on a 4X buffered Fourier Domain Mode Locked swept source laser with an A-scan rate of ~1.5 MHz. The elastic wave was imaged at a frame rate of ~7.3 kHz using only a single excitation. In contrast to previous techniques, successive B-scans were acquired over the measurement region (B-M mode) in this work. The feasibility of this method was validated by quantifying the elasticity of tissue-mimicking agar phantoms as well as porcine corneas ex vivo at different intraocular pressures. The results demonstrate that this method can acquire a depth-resolved elastogram in milliseconds. The reduced data set enabled a rapid elasticity assessment, and the ultra-fast acquisition speed allowed for a clinically safe laser exposure to the cornea.

Wavelength-encoded tomography based on optical temporal Fourier transform

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Chi; Wong, Kenneth K. Y., E-mail: kywong@eee.hku.hk

We propose and demonstrate a technique called wavelength-encoded tomography (WET) for non-invasive optical cross-sectional imaging, particularly beneficial in biological system. The WET utilizes time-lens to perform the optical Fourier transform, and the time-to-wavelength conversion generates a wavelength-encoded image of optical scattering from internal microstructures, analogous to the interferometery-based imaging such as optical coherence tomography. Optical Fourier transform, in principle, comes with twice as good axial resolution over the electrical Fourier transform, and will greatly simplify the digital signal processing after the data acquisition. As a proof-of-principle demonstration, a 150 -μm (ideally 36 μm) resolution is achieved based on a 7.5-nm bandwidth swept-pump,more » using a conventional optical spectrum analyzer. This approach can potentially achieve up to 100-MHz or even higher frame rate with some proven ultrafast spectrum analyzer. We believe that this technique is innovative towards the next-generation ultrafast optical tomographic imaging application.« less

Brady's Geothermal Field Nodal Seismometer Active Source Data Sample

DOE Data Explorer

Kurt Feigl

2016-03-25

This data is in sac format and includes recordings of two active source events from 238 three-component nodal seismometers deployed at Bradys Hot Springs geothermal field as part of the PoroTomo project. The source was a viberoseis truck operating in P-wave vibrational mode and generating a swept-frequency signal. The files are 33 seconds long starting 4 seconds before each sweep was initiated. There is some overlap in the file times.

Common path endoscopic probes for optical coherence tomography (Conference Presentation)

NASA Astrophysics Data System (ADS)

Singh, Kanwarpal; Gardecki, Joseph A.; Tearney, Guillermo J.

2017-02-01

Background: Dispersion imbalance and polarization mismatch between the reference and sample arm signals can lead to image quality degradation in optical coherence tomography (OCT). One approach to reduce these image artifacts is to employ a common-path geometry in fiber-based probes. In this work, we report an 800 um diameter all-fiber common-path monolithic probe for coronary artery imaging where the reference signal is generated using an inline fiber partial reflector. Methods: Our common-path probe was designed for swept-source based Fourier domain OCT at 1310 nm wavelength. A face of a coreless fiber was coated with gold and spliced to a standard SMF-28 single mode fiber creating an inline partial reflector, which acted as a reference surface. The other face of the coreless fiber was shaped into a ball lens for focusing. The optical elements were assembled within a 560 µm diameter drive shaft, which was attached to a rotary junction. The drive shaft was placed inside a transparent sheath having an outer diameter of 800 µm. Results: With a source input power of 30mW, the inline common-path probe achieved a sensitivity of 104 dB. Images of human finger skin showed the characteristic layers of skin as well as features such as sweat ducts. Images of coronary arteries ex vivo obtained with this probe enabled visualization of the characteristic architectural morphology of the normal artery wall and known features of atherosclerotic plaque. Conclusion: In this work, we have demonstrated a common path OCT probe for cardiovascular imaging. The probe is easy to fabricate, will reduce system complexity and overall cost. We believe that this design will be helpful in endoscopic applications that require high resolution and a compact form factor.

Real time display Fourier-domain OCT using multi-thread parallel computing with data vectorization

NASA Astrophysics Data System (ADS)

Eom, Tae Joong; Kim, Hoon Seop; Kim, Chul Min; Lee, Yeung Lak; Choi, Eun-Seo

2011-03-01

We demonstrate a real-time display of processed OCT images using multi-thread parallel computing with a quad-core CPU of a personal computer. The data of each A-line are treated as one vector to maximize the data translation rate between the cores of the CPU and RAM stored image data. A display rate of 29.9 frames/sec for processed OCT data (4096 FFT-size x 500 A-scans) is achieved in our system using a wavelength swept source with 52-kHz swept frequency. The data processing times of the OCT image and a Doppler OCT image with a 4-time average are 23.8 msec and 91.4 msec.

Vela X: A plerion or part of a shell?

NASA Astrophysics Data System (ADS)

Gvaramadze, V. V.

1998-03-01

An analysis of the radio, optical, and X-ray observations of the supernova remnant (SNR) in Vela has led us to conclude that the radio source Vela X is part of the SNR shell. The high brightness of this radio source is assumed to be a result of the interaction of dome-shaped deformations (bubbles) on the SNR shell, which gives rise to bright radio filaments. The deformations could be produced by Richtmaier-Meshkov's instability, which develops during the impulsive acceleration of a shell of gas (swept up from the interstellar medium by the wind from a presupernova) by a shock wave (generated by a supernova explosion). The brightest radio filament and the X-ray jet extending along it are shown to be located in the region of interaction of two prominent bubbles on the SNR shell. We conclude that the X-ray jet, like Vela X, is part of the shell, and that it has its origin in the Mach reflection of two semispherical shock waves. Our estimate of the plasma temperature behind the front of the Mach wave matches the jet temperature. We also show that the large spread in the estimates of the spectral index for Vela X could be caused by the instrumental effect which arises during observations of extended radio sources with a nonuniform surface-brightness distribution.

X-ray lasers and methods utilizing two component driving illumination provided by optical laser means of relatively low energy and small physical size

DOEpatents

Rosen, Mordecai D.; Matthews, Dennis L.

1991-01-01

An X-ray laser (10), and related methodology, are disclosed wherein an X-ray laser target (12) is illuminated with a first pulse of optical laser radiation (14) of relatively long duration having scarcely enough energy to produce a narrow and linear cool plasma of uniform composition (38). A second, relatively short pulse of optical laser radiation (18) is uniformly swept across the length, from end to end, of the plasma (38), at about the speed of light, to consecutively illuminate continuously succeeding portions of the plasma (38) with optical laser radiation having scarcely enough energy to heat, ionize, and invert them into the continuously succeeding portions of an X-ray gain medium. This inventive double pulse technique results in a saving of more than two orders of magnitude in driving optical laser energy, when compared to the conventional single pulse approach.

A beam optics study of a modular multi-source X-ray tube for novel computed tomography applications

NASA Astrophysics Data System (ADS)

Walker, Brandon J.; Radtke, Jeff; Chen, Guang-Hong; Eliceiri, Kevin W.; Mackie, Thomas R.

2017-10-01

A modular implementation of a scanning multi-source X-ray tube is designed for the increasing number of multi-source imaging applications in computed tomography (CT). An electron beam array coupled with an oscillating magnetic deflector is proposed as a means for producing an X-ray focal spot at any position along a line. The preliminary multi-source model includes three thermionic electron guns that are deflected in tandem by a slowly varying magnetic field and pulsed according to a scanning sequence that is dependent on the intended imaging application. Particle tracking simulations with particle dynamics analysis software demonstrate that three 100 keV electron beams are laterally swept a combined distance of 15 cm over a stationary target with an oscillating magnetic field of 102 G perpendicular to the beam axis. Beam modulation is accomplished using 25 μs pulse widths to a grid electrode with a reverse gate bias of -500 V and an extraction voltage of +1000 V. Projected focal spot diameters are approximately 1 mm for 138 mA electron beams and the stationary target stays within thermal limits for the 14 kW module. This concept could be used as a research platform for investigating high-speed stationary CT scanners, for lowering dose with virtual fan beam formation, for reducing scatter radiation in cone-beam CT, or for other industrial applications.

Optical Coherence Tomography Angiography of Optic Disc Perfusion in Glaucoma

PubMed Central

Jia, Yali; Wei, Eric; Wang, Xiaogang; Zhang, Xinbo; Morrison, John C.; Parikh, Mansi; Lombardi, Lori H.; Gattey, Devin M.; Armour, Rebecca L.; Edmunds, Beth; Kraus, Martin F.; Fujimoto, James G.; Huang, David

2014-01-01

Purpose To compare optic disc perfusion between normal and glaucoma subjects using optical coherence tomography (OCT) angiography and detect optic disc perfusion changes in glaucoma. Design Observational, cross-sectional study. Participants Twenty-four normal subjects and 11 glaucoma patients were included. Methods One eye of each subject was scanned by a high-speed 1050 nm wavelength swept-source OCT instrument. The split-spectrum amplitude-decorrelation angiography algorithm (SSADA) was used to compute three-dimensional optic disc angiography. A disc flow index was computed from four registered scans. Confocal scanning laser ophthalmoscopy (cSLO) was used to measure disc rim area, and stereo photography was used to evaluate cup/disc ratios. Wide field OCT scans over the discs were used to measure retinal nerve fiber layer (NFL) thickness. Main Outcome Measurements Variability was assessed by coefficient of variation (CV). Diagnostic accuracy was assessed by sensitivity and specificity. Comparisons between glaucoma and normal groups were analyzed by Wilcoxon rank-sum test. Correlations between disc flow index, structural assessments, and visual field (VF) parameters were assessed by linear regression. Results In normal discs, a dense microvascular network was visible on OCT angiography. This network was visibly attenuated in glaucoma subjects. The intra-visit repeatability, inter-visit reproducibility, and normal population variability of the optic disc flow index were 1.2%, 4.2%, and 5.0% CV respectively. The disc flow index was reduced by 25% in the glaucoma group (p = 0.003). Sensitivity and specificity were both 100% using an optimized cutoff. The flow index was highly correlated with VF pattern standard deviation (R2 = 0.752, p = 0.001). These correlations were significant even after accounting for age, cup/disc area ratio, NFL, and rim area. Conclusions OCT angiography, generated by the new SSADA algorithm, repeatably measures optic disc perfusion. OCT angiography could be useful in the evaluation of glaucoma and glaucoma progression. PMID:24629312

Spectral amplitude code label switching system for IM, DQPSK and PDM-DQPSK with frequency swept coherent detection

NASA Astrophysics Data System (ADS)

Isaac, Aboagye Adjaye; Yongsheng, Cao; Fushen, Chen

2018-05-01

We present and compare the outcome of implicit and explicit labels using intensity modulation (IM), differential quadrature phase shift keying (DQPSK), and polarization division multiplexed (PDM-DQPSK). A payload bit rate of 1, 2, and 5 Gb/s is considered for IM implicit labels, while payloads of 40, 80, and 112 Gb/s are considered in DQPSK and PDM-DQPSK explicit labels by stimulating a 4-code 156-Mb/s SAC label. The generated label and payloads are observed by assessing the eye diagram, received optical power (ROP), and optical signal to noise ratio (OSNR).

Implementations of three OCT angiography (OCTA) methods with 1.7 MHz A-scan rate OCT system on imaging of human retinal and choroidal vasculature

NASA Astrophysics Data System (ADS)

Poddar, Raju; Werner, John S.

2018-06-01

We present noninvasive depth-resolved imaging of human retinal and choroidal microcirculation with an ultrahigh-speed (1.7 MHz A-scans/s), Fourier-domain mode locked (FDML) swept-source optical coherence tomography (SS-OCT) system having a central wavelength of 1065 nm. Three OCT angiography (OCTA) motion based contrast methods, namely phase variance (PV), amplitude decorrelation (AD) and Joint Spectral and Time domain OCT (STdOCT) were implemented. The OCTA imaging was performed with a field of view of 16° (5 mm × 5 mm) and 30° (9 mm × 9 mm), on the retina. A qualitative comparison of images obtained with all three OCTA methods is demonstrated using the same eye of a healthy volunteer. Different parameters, namely acquisition time, scanning area, and scanning density, are discussed. The phase-variance OCTA (PV-OCTA) method produced relatively better results than the other two. Different features regarding the retinal and choroidal vessels are described in different subjects.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lee, Hee Yoon; Department of Otolaryngology-Head and Neck Surgery, Stanford University, Stanford, California; Raphael, Patrick D.

Cochlear amplification has been most commonly investigated by measuring the vibrations of the basilar membrane in animal models. Several different techniques have been used for measuring these vibrations such as laser Doppler vibrometry, miniature pressure sensors, low coherence interferometry, and spectral-domain optical coherence tomography (SD-OCT). We have built a swept-source OCT (SS-OCT) system, which is similar to SD-OCT in that it is capable of performing both imaging and vibration measurements within the mouse cochlea in vivo without having to open the bone. In vivo 3D images of a mouse cochlea were obtained, and the basilar membrane, tectorial membrane, Reissner’s membrane,more » tunnel of Corti, and reticular lamina could all be resolved. We measured vibrations of multiple structures within the mouse cochlea to sound stimuli. As well, we measured the radial deflections of the reticular lamina and tectorial membrane to estimate the displacement of the outer hair cell stereocilia. These measurements have the potential to more clearly define the mechanisms underlying the linear and non-linear processes within the mammalian cochlea.« less

High-resolution retinal swept source optical coherence tomography with an ultra-wideband Fourier-domain mode-locked laser at MHz A-scan rates

PubMed Central

Kolb, Jan Philip; Pfeiffer, Tom; Eibl, Matthias; Hakert, Hubertus; Huber, Robert

2017-01-01

We present a new 1060 nm Fourier domain mode locked laser (FDML laser) with a record 143 nm sweep bandwidth at 2∙ 417 kHz  =  834 kHz and 120 nm at 1.67 MHz, respectively. We show that not only the bandwidth alone, but also the shape of the spectrum is critical for the resulting axial resolution, because of the specific wavelength-dependent absorption of the vitreous. The theoretical limit of our setup lies at 5.9 µm axial resolution. In vivo MHz-OCT imaging of human retina is performed and the image quality is compared to the previous results acquired with 70 nm sweep range, as well as to existing spectral domain OCT data with 2.1 µm axial resolution from literature. We identify benefits of the higher resolution, for example the improved visualization of small blood vessels in the retina besides several others. PMID:29359091

High-resolution retinal swept source optical coherence tomography with an ultra-wideband Fourier-domain mode-locked laser at MHz A-scan rates.

PubMed

Kolb, Jan Philip; Pfeiffer, Tom; Eibl, Matthias; Hakert, Hubertus; Huber, Robert

2018-01-01

We present a new 1060 nm Fourier domain mode locked laser (FDML laser) with a record 143 nm sweep bandwidth at 2∙ 417 kHz  =  834 kHz and 120 nm at 1.67 MHz, respectively. We show that not only the bandwidth alone, but also the shape of the spectrum is critical for the resulting axial resolution, because of the specific wavelength-dependent absorption of the vitreous. The theoretical limit of our setup lies at 5.9 µm axial resolution. In vivo MHz-OCT imaging of human retina is performed and the image quality is compared to the previous results acquired with 70 nm sweep range, as well as to existing spectral domain OCT data with 2.1 µm axial resolution from literature. We identify benefits of the higher resolution, for example the improved visualization of small blood vessels in the retina besides several others.

Speckle variance OCT for depth resolved assessment of the viability of bovine embryos

PubMed Central

Caujolle, S.; Cernat, R.; Silvestri, G.; Marques, M. J.; Bradu, A.; Feuchter, T.; Robinson, G.; Griffin, D. K.; Podoleanu, A.

2017-01-01

The morphology of embryos produced by in vitro fertilization (IVF) is commonly used to estimate their viability. However, imaging by standard microscopy is subjective and unable to assess the embryo on a cellular scale after compaction. Optical coherence tomography is an imaging technique that can produce a depth-resolved profile of a sample and can be coupled with speckle variance (SV) to detect motion on a micron scale. In this study, day 7 post-IVF bovine embryos were observed either short-term (10 minutes) or long-term (over 18 hours) and analyzed by swept source OCT and SV to resolve their depth profile and characterize micron-scale movements potentially associated with viability. The percentage of en face images showing movement at any given time was calculated as a method to detect the vital status of the embryo. This method could be used to measure the levels of damage sustained by an embryo, for example after cryopreservation, in a rapid and non-invasive way. PMID:29188109

Binge consumption of ethanol during pregnancy leads to significant developmental delay of mouse embryonic brain

NASA Astrophysics Data System (ADS)

Sudheendran, Narendran; Bake, Shameena; Miranda, Rajesh C.; Larin, Kirill V.

2014-03-01

Consumption of alcohol during pregnancy can be severely detrimental to the development of the brain in fetuses. This study explores the usage of optical coherence tomography (OCT) to the study the effects of maternal consumption of ethanol on brain development in mouse fetuses. On gestational day 14.5, fetuses were collected and fixed in 4% paraformaldehyde. A swept-source OCT (SSOCT) system was used to acquire 3D images of the brain of ethanol-exposed and control fetuses. The volume of right and left brain ventricles were measured and used to compare between ethanol-exposed and control fetuses. A total of 5 fetuses were used for each of the two groups. The average volumes of the right and left ventricles were measured to be 0.35 and 0.15 mm3 for ethanol-exposed and control fetuses, respectively. The results demonstrated that there is an alcohol-induced developmental delay in mouse fetal brains.

Effect of alcohol exposure on fetal brain development

NASA Astrophysics Data System (ADS)

Sudheendran, Narendran; Bake, Shameena; Miranda, Rajesh C.; Larin, Kirill V.

2013-02-01

Alcohol consumption during pregnancy can be severely damage to the brain development in fetuses. This study investigates the effects of maternal ethanol consumption on brain development in mice embryos. Pregnant mice at gestational day 12.5 were intragastrically gavaged with ethanol (3g/Kg bwt) twice daily for three consecutive days. On gestational day 14.5, fetuses were collected and fixed in 4% paraformaldehyde and imaged using a swept-source optical coherence tomography (SSOCT) system. 3D images of the mice embryo brain were obtained and the volumes of the left and right ventricles of the brain were measured. The average volumes of the left and the right volumes of 5 embryos each alcohol-exposed and control embryos were measured to be 0.35 and 0.15 mm3, respectively. The results suggest that the left and right ventricle volumes of brain are much larger in the alcohol-exposed embryos as compared to control embryos indicating alcohol-induced developmental delay.

140 W peak power laser system tunable in the LWIR.

PubMed

Gutty, François; Grisard, Arnaud; Larat, Christian; Papillon, Dominique; Schwarz, Muriel; Gerard, Bruno; Ostendorf, Ralf; Rattunde, Marcel; Wagner, Joachim; Lallier, Eric

2017-08-07

We present a high peak power rapidly tunable laser system in the long-wave infrared comprising an external-cavity quantum cascade laser (EC-QCL) broadly tunable from 8 to 10 µm and an optical parametric amplifier (OPA) based on quasi phase-matching in orientation-patterned gallium arsenide (OP-GaAs) of fixed grating period. The nonlinear crystal is pumped by a pulsed fiber laser system to achieve efficient amplification in the OPA. Quasi phase-matching remains satisfied when the EC-QCL wavelength is swept from 8 to 10 µm with a crystal of fixed grating period through tuning the pump laser source around 2 µm. The OPA demonstrates parametric amplification from 8 µm to 10 µm and achieves output peak powers up to 140 W with spectral linewidths below 3.5 cm -1 . The beam profile quality (M 2 ) remains below 3.4 in both horizontal and vertical directions. Compared to the EC-QCL, the linewidth broadening is attributed to a coupling with the OPA.

High speed all optical shear wave imaging optical coherence elastography (Conference Presentation)

NASA Astrophysics Data System (ADS)

Song, Shaozhen; Hsieh, Bao-Yu; Wei, Wei; Shen, Tueng; O'Donnell, Matthew; Wang, Ruikang K.

2016-03-01

Optical Coherence Elastography (OCE) is a non-invasive testing modality that maps the mechanical property of soft tissues with high sensitivity and spatial resolution using phase-sensitive optical coherence tomography (PhS-OCT). Shear wave OCE (SW-OCE) is a leading technique that relies on the speed of propagating shear waves to provide a quantitative elastography. Previous shear wave imaging OCT techniques are based on repeated M-B scans, which have several drawbacks such as long acquisition time and repeated wave stimulations. Recent developments of Fourier domain mode-locked high-speed swept-source OCT system has enabled enough speed to perform KHz B-scan rate OCT imaging. Here we propose ultra-high speed, single shot shear wave imaging to capture single-shot transient shear wave propagation to perform SW-OCE. The frame rate of shear wave imaging is 16 kHz, at A-line rate of ~1.62 MHz, which allows the detection of high-frequency shear wave of up to 8 kHz. The shear wave is generated photothermal-acoustically, by ultra-violet pulsed laser, which requires no contact to OCE subjects, while launching high frequency shear waves that carries rich localized elasticity information. The image acquisition and processing can be performed at video-rate, which enables real-time 3D elastography. SW-OCE measurements are demonstrated on tissue-mimicking phantoms and porcine ocular tissue. This approach opens up the feasibility to perform real-time 3D SW-OCE in clinical applications, to obtain high-resolution localized quantitative measurement of tissue biomechanical property.

A Method for Determining Cloud-Droplet Impingement on Swept Wings

NASA Technical Reports Server (NTRS)

Dorsch, Robert G.; Brun, Rinaldo J.

1953-01-01

The general effect of wing sweep on cloud-droplet trajectories about swept wings of high aspect ratio moving at subsonic speeds is discussed. A method of computing droplet trajectories about yawed cylinders and swept wings is presented, and illustrative droplet trajectories are computed. A method of extending two-dimensional calculations of droplet impingement on nonswept wings to swept wings is presented. It is shown that the extent of impingement of cloud droplets on an airfoil surface, the total rate of collection of water, and the local rate of impingement per unit area of airfoil surface can be found for a swept wing from two-dimensional data for a nonswept wing. The impingement on a swept wing is obtained from impingement data for a nonswept airfoil section which is the same as the section in the normal plane of the swept wing by calculating all dimensionless parameters with respect to flow conditions in the normal plane of the swept wing.

STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF BENIGN FLECK RETINA USING MULTIMODAL IMAGING.

PubMed

Neriyanuri, Srividya; Rao, Chetan; Raman, Rajiv

2017-01-01

To report structural and functional features in a case series of benign fleck retina using multimodal imaging. Four cases with benign fleck retina underwent complete ophthalmic examination that included detailed history, visual acuity, and refractive error testing, FM-100 hue test, dilated fundus evaluation, full field electroretinogram, fundus photography with autofluorescence, fundus fluorescein angiography, and swept-source optical coherence tomography. Age group of the cases ranged from 19 years to 35 years (3 males and 1 female). Parental consanguinity was reported in two cases. All of them were visually asymptomatic with best-corrected visual acuity of 20/20 (moderate astigmatism) in both the eyes. Low color discrimination was seen in two cases. Fundus photography showed pisciform flecks which were compactly placed on posterior pole and were discrete, diverging towards periphery. Lesions were seen as smaller dots within 1500 microns from fovea and were hyperfluorescent on autofluorescence. Palisading retinal pigment epithelium defects were seen in posterior pole on fundus fluorescein angiography imaging; irregular hyper fluorescence was also noted. One case had reduced cone responses on full field electroretinogram; the other three cases had normal electroretinogram. On optical coherence tomography, level of lesions varied from retinal pigment epithelium, inner segment to outer segment extending till external limiting membrane. Functional and structural deficits in benign fleck retina were picked up using multimodal imaging.

Lamellar keratoplasty using position-guided surgical needle and M-mode optical coherence tomography

NASA Astrophysics Data System (ADS)

Shin, Sungwon; Bae, Jung Kweon; Ahn, Yujin; Kim, Hyeongeun; Choi, Geonho; Yoo, Young-Sik; Joo, Choun-Ki; Moon, Sucbei; Jung, Woonggyu

2017-12-01

Deep anterior lamellar keratoplasty (DALK) is an emerging surgical technique for the restoration of corneal clarity and vision acuity. The big-bubble technique in DALK surgery is the most essential procedure that includes the air injection through a thin syringe needle to separate the dysfunctional region of the cornea. Even though DALK is a well-known transplant method, it is still challenged to manipulate the needle inside the cornea under the surgical microscope, which varies its surgical yield. Here, we introduce the DALK protocol based on the position-guided needle and M-mode optical coherence tomography (OCT). Depth-resolved 26-gage needle was specially designed, fabricated by the stepwise transitional core fiber, and integrated with the swept source OCT system. Since our device is feasible to provide both the position information inside the cornea as well as air injection, it enables the accurate management of bubble formation during DALK. Our results show that real-time feedback of needle end position was intuitionally visualized and fast enough to adjust the location of the needle. Through our research, we realized that position-guided needle combined with M-mode OCT is a very efficient and promising surgical tool, which also to enhance the accuracy and stability of DALK.

Circumferential optical coherence tomography angiography imaging of the swine esophagus using a micromotor balloon catheter

PubMed Central

Lee, Hsiang-Chieh; Ahsen, Osman Oguz; Liang, Kaicheng; Wang, Zhao; Cleveland, Cody; Booth, Lucas; Potsaid, Benjamin; Jayaraman, Vijaysekhar; Cable, Alex E.; Mashimo, Hiroshi; Langer, Robert; Traverso, Giovanni; Fujimoto, James G.

2016-01-01

We demonstrate a micromotor balloon imaging catheter for ultrahigh speed endoscopic optical coherence tomography (OCT) which provides wide area, circumferential structural and angiographic imaging of the esophagus without contrast agents. Using a 1310 nm MEMS tunable wavelength swept VCSEL light source, the system has a 1.2 MHz A-scan rate and ~8.5 µm axial resolution in tissue. The micromotor balloon catheter enables circumferential imaging of the esophagus at 240 frames per second (fps) with a ~30 µm (FWHM) spot size. Volumetric imaging is achieved by proximal pullback of the micromotor assembly within the balloon at 1.5 mm/sec. Volumetric data consisting of 4200 circumferential images of 5,000 A-scans each over a 2.6 cm length, covering a ~13 cm2 area is acquired in <18 seconds. A non-rigid image registration algorithm is used to suppress motion artifacts from non-uniform rotational distortion (NURD), cardiac motion or respiration. En face OCT images at various depths can be generated. OCT angiography (OCTA) is computed using intensity decorrelation between sequential pairs of circumferential scans and enables three-dimensional visualization of vasculature. Wide area volumetric OCT and OCTA imaging of the swine esophagus in vivo is demonstrated. PMID:27570688

Anterior Chamber Angle Shape Analysis and Classification of Glaucoma in SS-OCT Images.

PubMed

Ni Ni, Soe; Tian, J; Marziliano, Pina; Wong, Hong-Tym

2014-01-01

Optical coherence tomography is a high resolution, rapid, and noninvasive diagnostic tool for angle closure glaucoma. In this paper, we present a new strategy for the classification of the angle closure glaucoma using morphological shape analysis of the iridocorneal angle. The angle structure configuration is quantified by the following six features: (1) mean of the continuous measurement of the angle opening distance; (2) area of the trapezoidal profile of the iridocorneal angle centered at Schwalbe's line; (3) mean of the iris curvature from the extracted iris image; (4) complex shape descriptor, fractal dimension, to quantify the complexity, or changes of iridocorneal angle; (5) ellipticity moment shape descriptor; and (6) triangularity moment shape descriptor. Then, the fuzzy k nearest neighbor (fkNN) classifier is utilized for classification of angle closure glaucoma. Two hundred and sixty-four swept source optical coherence tomography (SS-OCT) images from 148 patients were analyzed in this study. From the experimental results, the fkNN reveals the best classification accuracy (99.11 ± 0.76%) and AUC (0.98 ± 0.012) with the combination of fractal dimension and biometric parameters. It showed that the proposed approach has promising potential to become a computer aided diagnostic tool for angle closure glaucoma (ACG) disease.

Motion-compensated optical coherence tomography using envelope-based surface detection and Kalman-based prediction

NASA Astrophysics Data System (ADS)

Irsch, Kristina; Lee, Soohyun; Bose, Sanjukta N.; Kang, Jin U.

2018-02-01

We present an optical coherence tomography (OCT) imaging system that effectively compensates unwanted axial motion with micron-scale accuracy. The OCT system is based on a swept-source (SS) engine (1060-nm center wavelength, 100-nm full-width sweeping bandwidth, and 100-kHz repetition rate), with axial and lateral resolutions of about 4.5 and 8.5 microns respectively. The SS-OCT system incorporates a distance sensing method utilizing an envelope-based surface detection algorithm. The algorithm locates the target surface from the B-scans, taking into account not just the first or highest peak but the entire signature of sequential A-scans. Subsequently, a Kalman filter is applied as predictor to make up for system latencies, before sending the calculated position information to control a linear motor, adjusting and maintaining a fixed system-target distance. To test system performance, the motioncorrection algorithm was compared to earlier, more basic peak-based surface detection methods and to performing no motion compensation. Results demonstrate increased robustness and reproducibility, particularly noticeable in multilayered tissues, while utilizing the novel technique. Implementing such motion compensation into clinical OCT systems may thus improve the reliability of objective and quantitative information that can be extracted from OCT measurements.

Optical coherence tomography for blood glucose monitoring in vitro through spatial and temporal approaches

NASA Astrophysics Data System (ADS)

De Pretto, Lucas Ramos; Yoshimura, Tania Mateus; Ribeiro, Martha Simões; Zanardi de Freitas, Anderson

2016-08-01

As diabetes causes millions of deaths worldwide every year, new methods for blood glucose monitoring are in demand. Noninvasive approaches may increase patient adherence to treatment while reducing costs, and optical coherence tomography (OCT) may be a feasible alternative to current invasive diagnostics. This study presents two methods for blood sugar monitoring with OCT in vitro. The first, based on spatial statistics, exploits changes in the light total attenuation coefficient caused by different concentrations of glucose in the sample using a 930-nm commercial OCT system. The second, based on temporal analysis, calculates differences in the decorrelation time of the speckle pattern in the OCT signal due to blood viscosity variations with the addition of glucose with data acquired by a custom built Swept Source 1325-nm OCT system. Samples consisted of heparinized mouse blood, phosphate buffer saline, and glucose. Additionally, further samples were prepared by diluting mouse blood with isotonic saline solution to verify the effect of higher multiple scattering components on the ability of the methods to differentiate glucose levels. Our results suggest a direct relationship between glucose concentration and both decorrelation rate and attenuation coefficient, with our systems being able to detect changes of 65 mg/dL in glucose concentration.

Monitoring remineralization of enamel subsurface lesions by optical coherence tomography

NASA Astrophysics Data System (ADS)

Mandurah, Mona M.; Sadr, Alireza; Shimada, Yasushi; Kitasako, Yuichi; Nakashima, Syozi; Bakhsh, Turki A.; Tagami, Junji; Sumi, Yasunori

2013-04-01

Optical coherence tomography (OCT) is a potential clinical tool for enamel lesion monitoring. Swept-source OCT findings were compared with cross-sectional nanohardness findings of enamel. Subsurface bovine enamel lesions in three groups were subjected to (1) deionized water (control), (2) phosphoryl oligosaccharide of calcium (POs-Ca) or (3) POs-Ca with 1 ppm fluoride for 14 days. B-scans images were obtained at 1310-nm center wavelength on sound, demineralized and remineralized areas after 4, 7, and 14 days. The specimens were processed for cross-sectional nanoindentation. Reflectivity from enamel that had increased with demineralization decreased with remineralization. An OCT attenuation coefficient parameter (μt), derived based on the Beer-Lambert law as a function of backscatter signal slope, showed a strong linear regression with integrated nanohardness of all regions (p<0.001, r=-0.97). Sound enamel showed the smallest, while demineralized enamel showed the highest μt. In group three, μt was significantly lower at four days than baseline, but remained constant afterwards. In group two, the changes were rather gradual. There was no significant difference between groups two and three at 14 days in nanohardness or μt POs-Ca with fluoride-enhanced nanohardness of the superficial zone. OCT signal attenuation demonstrated a capability for monitoring changes of enamel lesions during remineralization.

Shock Characteristics Measured Upstream of Both a Forward-Swept and an Aft-Swept Fan

NASA Technical Reports Server (NTRS)

Podboy, Gary G.; Krupar, Martin J.; Sutliff, Daniel L.; Horvath, Csaba

2007-01-01

Three different types of diagnostic data-blade surface flow visualization, shroud unsteady pressure, and laser Doppler velocimeter (LDV)--were obtained on two fans, one forward-swept and one aft-swept, in order to learn more about the shocks which propagate upstream of these rotors when they are operated at transonic tip speeds. Flow visualization data are presented for the forward-swept fan operating at 13831 rpm(sub c), and for the aft-swept fan operating at 12500 and 13831 rpm(sub c) (corresponding to tip rotational Mach numbers of 1.07 and 1.19, respectively). The flow visualization data identify where the shocks occur on the suction side of the rotor blades. These data show that at the takeoff speed, 13831 rpm(sub c), the shocks occurring in the tip region of the forward-swept fan are further downstream in the blade passage than with the aft-swept fan. Shroud unsteady pressure measurements were acquired using a linear array of 15 equally-spaced pressure transducers extending from two tip axial chords upstream to 0.8 tip axial chords downstream of the static position of the tip leading edge of each rotor. Such data are presented for each fan operating at one subsonic and five transonic tip speeds. The unsteady pressure data show relatively strong detached shocks propagating upstream of the aft-swept rotor at the three lowest transonic tip speeds, and weak, oblique pressure disturbances attached to the tip of the aft-swept fan at the two highest transonic tip speeds. The unsteady pressure measurements made with the forward-swept fan do not show strong shocks propagating upstream of that rotor at any of the tested speeds. A comparison of the forward-swept and aft-swept shroud unsteady pressure measurements indicates that at any given transonic speed the pressure disturbance just upstream of the tip of the forward-swept fan is much weaker than that of the aft-swept fan. The LDV data suggest that at 12500 and 13831 rpm(sub c), the forward-swept fan swallowed the passage shocks occurring in the tip region of the blades, whereas the aft-swept fan did not. Due to this difference, the flows just upstream of the two fans were found to be quite different at both of these transonic speeds. Nevertheless, despite distinct differences just upstream of the two rotors, the two fan flows were much more alike about one axial blade chord further upstream. As a result, the LDV data suggest that it is unwise to attempt to determine the effect that the shocks have on far field noise by focusing only on measurements (or CFD predictions) made very near the rotor. Instead, these data suggest that it is important to track the shocks throughout the inlet.

Fatigue Damage Monitoring of a Composite Step Lap Joint Using Distributed Optical Fibre Sensors

PubMed Central

Wong, Leslie; Chowdhury, Nabil; Wang, John; Chiu, Wing Kong; Kodikara, Jayantha

2016-01-01

Over the past few decades, there has been a considerable interest in the use of distributed optical fibre sensors (DOFS) for structural health monitoring of composite structures. In aerospace-related work, health monitoring of the adhesive joints of composites has become more significant, as they can suffer from cracking and delamination, which can have a significant impact on the integrity of the joint. In this paper, a swept-wavelength interferometry (SWI) based DOFS technique is used to monitor the fatigue in a flush step lap joint composite structure. The presented results will show the potential application of distributed optical fibre sensor for damage detection, as well as monitoring the fatigue crack growth along the bondline of a step lap joint composite structure. The results confirmed that a distributed optical fibre sensor is able to enhance the detection of localised damage in a structure. PMID:28773496

Advanced intensity-modulation continuous-wave lidar techniques for ASCENDS CO2 column measurements

NASA Astrophysics Data System (ADS)

Campbell, Joel F.; Lin, Bing; Nehrir, Amin R.; Harrison, F. W.; Obland, Michael D.; Meadows, Byron

2015-10-01

Global atmospheric carbon dioxide (CO2) measurements for the NASA Active Sensing of CO2 Emissions over Nights, Days, and Seasons (ASCENDS) space mission are critical for improving our understanding of global CO2 sources and sinks. Advanced Intensity- Modulated Continuous-Wave (IM-CW) lidar techniques are investigated as a means of facilitating CO2 measurements from space to meet the ASCENDS measurement requirements. In recent numerical, laboratory and flight experiments we have successfully used the Binary Phase Shift Keying (BPSK) modulation technique to uniquely discriminate surface lidar returns from intermediate aerosol and cloud contamination. We demonstrate the utility of BPSK to eliminate sidelobes in the range profile as a means of making Integrated Path Differential Absorption (IPDA) column CO2 measurements in the presence of optically thin clouds, thereby eliminating the need to correct for sidelobe bias errors caused by the clouds. Furthermore, high accuracy and precision ranging to the surface as well as to the top of intermediate cloud layers, which is a requirement for the inversion of column CO2 number density measurements to column CO2 mixing ratios, has been demonstrated using new hyperfine interpolation techniques that takes advantage of the periodicity of the modulation waveforms. This approach works well for both BPSK and linear swept-frequency modulation techniques. The BPSK technique under investigation has excellent auto-correlation properties while possessing a finite bandwidth. A comparison of BPSK and linear swept-frequency is also discussed in this paper. These results are extended to include Richardson-Lucy deconvolution techniques to extend the resolution of the lidar beyond that implied by limit of the bandwidth of the modulation, where it is shown useful for making tree canopy measurements.

Advanced IMCW Lidar Techniques for ASCENDS CO2 Column Measurements

NASA Astrophysics Data System (ADS)

Campbell, Joel; lin, bing; nehrir, amin; harrison, fenton; obland, michael

2015-04-01

Global atmospheric carbon dioxide (CO2) measurements for the NASA Active Sensing of CO2 Emissions over Nights, Days, and Seasons (ASCENDS) space mission are critical for improving our understanding of global CO2 sources and sinks. Advanced Intensity-Modulated Continuous-Wave (IM-CW) lidar techniques are investigated as a means of facilitating CO2 measurements from space to meet the ASCENDS measurement requirements. In recent numerical, laboratory and flight experiments we have successfully used the Binary Phase Shift Keying (BPSK) modulation technique to uniquely discriminate surface lidar returns from intermediate aerosol and cloud contamination. We demonstrate the utility of BPSK to eliminate sidelobes in the range profile as a means of making Integrated Path Differential Absorption (IPDA) column CO2 measurements in the presence of optically thin clouds, thereby eliminating the need to correct for sidelobe bias errors caused by the clouds. Furthermore, high accuracy and precision ranging to the surface as well as to the top of intermediate cloud layers, which is a requirement for the inversion of column CO2 number density measurements to column CO2 mixing ratios, has been demonstrated using new hyperfine interpolation techniques that takes advantage of the periodicity of the modulation waveforms. This approach works well for both BPSK and linear swept-frequency modulation techniques. The BPSK technique under investigation has excellent auto-correlation properties while possessing a finite bandwidth. A comparison of BPSK and linear swept-frequency is also discussed in this paper. These results are extended to include Richardson-Lucy deconvolution techniques to extend the resolution of the lidar beyond that implied by limit of the bandwidth of the modulation.

Advanced Intensity-Modulation Continuous-Wave Lidar Techniques for ASCENDS O2 Column Measurements

NASA Technical Reports Server (NTRS)

Campbell, Joel F.; Lin, Bing; Nehrir, Amin R.; Harrison, F. Wallace; Obland, Michael D.; Meadows, Byron

2015-01-01

Global atmospheric carbon dioxide (CO2) measurements for the NASA Active Sensing of CO2 Emissions over Nights, Days, and Seasons (ASCENDS) space mission are critical for improving our understanding of global CO2 sources and sinks. Advanced Intensity- Modulated Continuous-Wave (IM-CW) lidar techniques are investigated as a means of facilitating CO2 measurements from space to meet the ASCENDS measurement requirements. In recent numerical, laboratory and flight experiments we have successfully used the Binary Phase Shift Keying (BPSK) modulation technique to uniquely discriminate surface lidar returns from intermediate aerosol and cloud contamination. We demonstrate the utility of BPSK to eliminate sidelobes in the range profile as a means of making Integrated Path Differential Absorption (IPDA) column CO2 measurements in the presence of optically thin clouds, thereby eliminating the need to correct for sidelobe bias errors caused by the clouds. Furthermore, high accuracy and precision ranging to the surface as well as to the top of intermediate cloud layers, which is a requirement for the inversion of column CO2 number density measurements to column CO2 mixing ratios, has been demonstrated using new hyperfine interpolation techniques that takes advantage of the periodicity of the modulation waveforms. This approach works well for both BPSK and linear swept-frequency modulation techniques. The BPSK technique under investigation has excellent auto-correlation properties while possessing a finite bandwidth. A comparison of BPSK and linear swept-frequency is also discussed in this paper. These results are extended to include Richardson-Lucy deconvolution techniques to extend the resolution of the lidar beyond that implied by limit of the bandwidth of the modulation, where it is shown useful for making tree canopy measurements.

Advanced Intensity-Modulation Continuous-Wave Lidar Techniques for Column CO2 Measurements

NASA Astrophysics Data System (ADS)

Campbell, J. F.; Lin, B.; Nehrir, A. R.; Obland, M. D.; Liu, Z.; Browell, E. V.; Chen, S.; Kooi, S. A.; Fan, T. F.

2015-12-01

Global and regional atmospheric carbon dioxide (CO2) measurements for the NASA Active Sensing of CO2 Emissions over Nights, Days, and Seasons (ASCENDS) space mission and Atmospheric Carbon and Transport (ACT) - America airborne investigation are critical for improving our understanding of global CO2 sources and sinks. Advanced Intensity-Modulated Continuous-Wave (IM-CW) lidar techniques are being investigated as a means of facilitating CO2 measurements from space and airborne platforms to meet the mission science measurement requirements. In recent numerical, laboratory and flight experiments we have successfully used the Binary Phase Shift Keying (BPSK) modulation technique to uniquely discriminate surface lidar returns from intermediate aerosol and cloud returns. We demonstrate the utility of BPSK to eliminate sidelobes in the range profile as a means of making Integrated Path Differential Absorption (IPDA) column CO2 measurements in the presence of intervening optically thin clouds, thereby minimizing bias errors caused by the clouds. Furthermore, high accuracy and precision ranging to the Earth's surface as well as to the top of intermediate cloud layers, which is a requirement for the inversion of column CO2 number density measurements to column CO2 mixing ratios, has been demonstrated using new hyperfine interpolation techniques that takes advantage of the periodicity of the modulation waveforms. This approach works well for both BPSK and linear swept-frequency modulation techniques and provides very high (at sub-meter level) range resolution. The BPSK technique under investigation has excellent auto-correlation properties while possessing a finite bandwidth. A comparison of BPSK and linear swept-frequency is also discussed in this paper. These techniques are used in a new data processing architecture to support the ASCENDS CarbonHawk Experiment Simulator (ACES) and ACT-America programs.

Viscoelastic characterization of dispersive media by inversion of a general wave propagation model in optical coherence elastography

NASA Astrophysics Data System (ADS)

Zvietcovich, Fernando; Rolland, Jannick P.; Grygotis, Emma; Wayson, Sarah; Helguera, Maria; Dalecki, Diane; Parker, Kevin J.

2018-02-01

Determining the mechanical properties of tissue such as elasticity and viscosity is fundamental for better understanding and assessment of pathological and physiological processes. Dynamic optical coherence elastography uses shear/surface wave propagation to estimate frequency-dependent wave speed and Young's modulus. However, for dispersive tissues, the displacement pulse is highly damped and distorted during propagation, diminishing the effectiveness of peak tracking approaches. The majority of methods used to determine mechanical properties assume a rheological model of tissue for the calculation of viscoelastic parameters. Further, plane wave propagation is sometimes assumed which contributes to estimation errors. To overcome these limitations, we invert a general wave propagation model which incorporates (1) the initial force shape of the excitation pulse in the space-time field, (2) wave speed dispersion, (3) wave attenuation caused by the material properties of the sample, (4) wave spreading caused by the outward cylindrical propagation of the wavefronts, and (5) the rheological-independent estimation of the dispersive medium. Experiments were conducted in elastic and viscous tissue-mimicking phantoms by producing a Gaussian push using acoustic radiation force excitation, and measuring the wave propagation using a swept-source frequency domain optical coherence tomography system. Results confirm the effectiveness of the inversion method in estimating viscoelasticity in both the viscous and elastic phantoms when compared to mechanical measurements. Finally, the viscoelastic characterization of collagen hydrogels was conducted. Preliminary results indicate a relationship between collagen concentration and viscoelastic parameters which is important for tissue engineering applications.

Retinal angiography with real-time speckle variance optical coherence tomography.

PubMed

Xu, Jing; Han, Sherry; Balaratnasingam, Chandrakumar; Mammo, Zaid; Wong, Kevin S K; Lee, Sieun; Cua, Michelle; Young, Mei; Kirker, Andrew; Albiani, David; Forooghian, Farzin; Mackenzie, Paul; Merkur, Andrew; Yu, Dao-Yi; Sarunic, Marinko V

2015-10-01

This report describes a novel, non-invasive and label-free optical imaging technique, speckle variance optical coherence tomography (svOCT), for visualising blood flow within human retinal capillary networks. This imaging system uses a custom-built swept source OCT system operating at a line rate of 100 kHz. Real-time processing and visualisation is implemented on a consumer grade graphics processing unit. To investigate the quality of microvascular detail acquired with this device we compared images of human capillary networks acquired with svOCT and fluorescein angiography. We found that the density of capillary microvasculature acquired with this svOCT device was visibly greater than fluorescein angiography. We also found that this svOCT device had the capacity to generate en face images of distinct capillary networks that are morphologically comparable with previously published histological studies. Finally, we found that this svOCT device has the ability to non-invasively illustrate the common manifestations of diabetic retinopathy and retinal vascular occlusion. The results of this study suggest that graphics processing unit accelerated svOCT has the potential to non-invasively provide useful quantitative information about human retinal capillary networks. Therefore svOCT may have clinical and research applications for the management of retinal microvascular diseases, which are a major cause of visual morbidity worldwide. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Experimental Water Droplet Impingement Data on Airfoils, Simulated Ice Shapes, an Engine Inlet and a Finite Wing

NASA Technical Reports Server (NTRS)

Papadakis, M.; Breer, M.; Craig, N.; Liu, X.

1994-01-01

An experimental method has been developed to determine the water droplet impingement characteristics on two- and three-dimensional aircraft surfaces. The experimental water droplet impingement data are used to validate particle trajectory analysis codes that are used in aircraft icing analyses and engine inlet particle separator analyses. The aircraft surface is covered with thin strips of blotter paper in areas of interest. The surface is then exposed to an airstream that contains a dyed-water spray cloud. The water droplet impingement data are extracted from the dyed blotter paper strips by measuring the optical reflectance of each strip with an automated reflectometer. Experimental impingement efficiency data represented for a NLF (1)-0414 airfoil, a swept MS (1)-0317 airfoil, a Boeing 737-300 engine inlet model, two simulated ice shapes and a swept NACA 0012 wingtip. Analytical impingement efficiency data are also presented for the NLF (1)-0414 airfoil and the Boeing 737-300 engine inlet model.

Aeroelastically coupled blades for vertical axis wind turbines

DOEpatents

Paquette, Joshua; Barone, Matthew F.

2016-02-23

Various technologies described herein pertain to a vertical axis wind turbine blade configured to rotate about a rotation axis. The vertical axis wind turbine blade includes at least an attachment segment, a rear swept segment, and optionally, a forward swept segment. The attachment segment is contiguous with the forward swept segment, and the forward swept segment is contiguous with the rear swept segment. The attachment segment includes a first portion of a centroid axis, the forward swept segment includes a second portion of the centroid axis, and the rear swept segment includes a third portion of the centroid axis. The second portion of the centroid axis is angularly displaced ahead of the first portion of the centroid axis and the third portion of the centroid axis is angularly displaced behind the first portion of the centroid axis in the direction of rotation about the rotation axis.

A swept wing panel in a low speed flexible walled test section

NASA Technical Reports Server (NTRS)

Goodyer, M. J.

1987-01-01

The testing of two-dimensional airfoil sections in adaptive wall tunnels is relatively widespread and has become routine at all speeds up to transonic. In contrast, the experience with the three-dimensional testing of swept panels in adaptive wall test sections is very limited, except for some activity in the 1940's at NPL, London. The current interest in testing swept wing panels led to the work covered by this report, which describes the design of an adaptive-wall swept-wing test section for a low speed wind tunnel and gives test results for a wing panel swept at 40 deg. The test section has rigid flat sidewalls supporting the panel, and features flexible top and bottom wall with ribs swept at the same angle as the wing. When streamlined, the walls form waves swept at the same angle as the wing. The C sub L (-) curve for the swept wing, determined from its pressure distributions taken with the walls streamlined, compare well with reference data which was taken on the same model, unswept, in a test section deep enough to avoid wall interference.

Frequency domain photothermoacoustic signal amplitude dependence on the optical properties of water: turbid polyvinyl chloride-plastisol system.

PubMed

Spirou, Gloria M; Mandelis, Andreas; Vitkin, I Alex; Whelan, William M

2008-05-10

Photoacoustic (more precisely, photothermoacoustic) signals generated by the absorption of photons can be related to the incident laser fluence rate. The dependence of frequency domain photoacoustic (FD-PA) signals on the optical absorption coefficient (micro(a)) and the effective attenuation coefficient (micro(eff)) of a turbid medium [polyvinyl chloride-plastisol (PVCP)] with tissuelike optical properties was measured, and empirical relationships between these optical properties and the photoacoustic (PA) signal amplitude and the laser fluence rate were derived for the water (PVCP system with and without optical scatterers). The measured relationships between these sample optical properties and the PA signal amplitude were found to be linear, consistent with FD-PA theory: micro(a)=a(A/Phi)-b and micro(eff)=c(A/Phi)+d, where Phi is the laser fluence, A is the FD-PA amplitude, and a, ...,d are empirical coefficients determined from the experiment using linear frequency-swept modulation and a lock-in heterodyne detection technique. This quantitative technique can easily be used to measure the optical properties of general turbid media using FD-PAs.

Wavelength-stepped, actively mode-locked fiber laser based on wavelength-division-multiplexed optical delay lines

NASA Astrophysics Data System (ADS)

Lee, Eunjoo; Kim, Byoung Yoon

2017-12-01

We propose a new scheme for an actively mode-locked wavelength-swept fiber laser that produces a train of discretely wavelength-stepped pulses from a short fiber cavity. Pulses with different wavelengths are split and combined by standard wavelength division multiplexers with fiber delay lines. As a proof of concept, we demonstrate a laser using an erbium doped fiber amplifier and commercially available wavelength-division multiplexers with wavelength spacing of 0.8 nm. The results show simultaneous mode-locking at three different wavelengths. Laser output parameters in time domain, optical and radio frequency spectral domain, and the noise characteristics are presented. Suggestions for the improved design are discussed.

Comparison of Far-Field Noise for Three Significantly Different Model Turbofans

NASA Technical Reports Server (NTRS)

Woodward, Richard P.

2008-01-01

Far-field noise sound power level (PWL) spectra and overall sound pressure level (OASPL) directivities were compared for three significantly different model fan stages which were tested in the NASA Glenn 9x15 Low Speed Wind Tunnel. The test fans included the Advanced Ducted Propulsor (ADP) Fan1, the baseline Source Diagnostic Test (SDT) fan, and the Quiet High Speed Fan2 (QHSF2) These fans had design rotor tangential tip speeds from 840 to 1474 ft/s and stage pressure ratios from 1.29 to 1.82. Additional parameters included rotor-stator spacing, stator sweep, and downstream support struts. Acoustic comparison points were selected on the basis of stage thrust. Acoustic results for the low tip speed/low pressure ratio fan (ADP Fan1) were thrust-adjusted to show how a geometrically-scaled version of this fan might compare at the higher design thrust levels of the other two fans. Lowest noise levels were typically observed for ADP Fan1 (which had a radial stator) and for the intermediate tip speed fan (Source Diagnostics Test, SDT, R4 rotor) with a swept stator. Projected noise levels for the ADP fan to the SDT swept stator configuration at design point conditions showed the fans to have similar noise levels. However, it is possible that the ADP fan could be 2 to 3 dB quieter with incorporation of a swept stator. Benefits of a scaled ADP fan include avoidance of multiple pure tones associated with transonic and higher blade tip speeds. Penalties of a larger size ADP fan would include increased nacelle size and drag.

Comparison of Far-field Noise for Three Significantly Different Model Turbofans

NASA Technical Reports Server (NTRS)

Woodward, Richard P.

2008-01-01

Far-field noise sound power level (PWL) spectra and overall sound pressure level (OASPL) directivities were compared for three significantly different model fan stages which were tested in the NASA Glenn 9 15 Low Speed Wind Tunnel. The test fans included the Advanced Ducted Propulsor (ADP) Fan1, the baseline Source Diagnostic Test (SDT) fan, and the Quiet High Speed Fan2 (QHSF2). These fans had design rotor tangential tip speeds from 840 to 1474 ft/s and stage pressure ratios from 1.29 to 1.82. Additional parameters included rotor-stator spacing, stator sweep, and downstream support struts. Acoustic comparison points were selected on the basis of stage thrust. Acoustic results for the low tip speed/low pressure ratio fan (ADP Fan1) were thrust-adjusted to show how a geometrically-scaled version of this fan might compare at the higher design thrust levels of the other two fans. Lowest noise levels were typically observed for ADP Fan1 (which had a radial stator) and for the intermediate tip speed fan (Source Diagnostics Test, SDT, R4 rotor) with a swept stator. Projected noise levels for the ADP fan to the SDT swept stator configuration at design point conditions showed the fans to have similar noise levels. However, it is possible that the ADP fan could be 2 to 3 dB quieter with incorporation of a swept stator. Benefits of a scaled ADP fan include avoidance of multiple pure tones associated with transonic and higher blade tip speeds. Penalties of a larger size ADP fan would include increased nacelle size and drag.

Measurement of Choroidal Perfusion and Thickness Following Systemic Sildenafil (Viagra®)

PubMed Central

Kim, David Y.; Silverman, Ronald H.; Chan, R.V. Paul; Khanifar, Aziz A.; Rondeau, Mark; Lloyd, Harriet; Schlegel, Peter; Coleman, D. Jackson

2011-01-01

Objective To demonstrate anatomic and physiologic changes in the human choroid following systemic sildenafil citrate (ViagraR) using enhanced depth imaging spectral domain-optical coherence tomography (EDI-OCT) and swept-scan high frequency digital ultrasound. Methods Seven healthy male subjects (mean age 32.7 years) were evaluated at baseline and two hours after ingesting 50 mg of sildenafil. Swept-scan high frequency digital ultrasound and EDI-OCT were utilized to measure choroidal perfusion and thickness, respectively. Results were read by masked observers. The Wilcoxon signed-rank test and t-test were used to analyze differences in choroidal flow and thickness at baseline and two hours after ingestion of sildenafil. Results Two hours following sildenafil, increased choroidal perfusion was observed in 11 of 12 eyes measured by swept-scan high frequency digital ultrasound. The mean increase was 3.46 (±2.00) times baseline with a range of 0.47 to 7.80 times baseline (p=0.004). Increased choroidal thickness was observed in 12 of 12 eyes measured with EDI-OCT. The average choroidal thickness increased by 11.6% temporal to the fovea, 9.3% nasal to the fovea, and 10.7% underneath the fovea (p<0.001 for all values). Conclusions Choroidal perfusion and thickness both increase in response to systemic sildenafil. These changes could secondarily affect retinal function, explain previously reported clinical symptoms, and potentially be a useful adjunct for treatment of ocular diseases that would benefit from increased choroidal blood flow. PMID:22974308

Formation of the lunar crust - An electrical source of heating

NASA Technical Reports Server (NTRS)

Sonett, C. P.; Colburn, D. S.; Schwartz, K.

1975-01-01

A model for formation of the lunar crust based on heating by electrical induction is explored, while adherence is maintained to certain constraints associated with existing models of the solar system. The heating mechanism is based on eddy current induction from disordered magnetic fields swept outwards by an intense (T Tauri-like) plasma flow from the sun. The electrical theory is an alternative to intense short-period accretion as a source of heat for the evolution of lunar maria and highlands, provided that long-lived radioactives are not swept to the surface from too large a melt volume during the initial thermal episode. This formation of the lunar highlands does not intrinsically require rapid accretion, nor on this basis is the time of formation of the planets generally restricted to a very short time. The threshold temperature for eddy current heating is attained by either a solar nebula at 300-400 C during formation of the moon or a very low energy long-period accumulation of the moon, both leading to melting in ten to the fifth to ten to the seventh power years.

Challenges of Zinc-Specific Transrectal Fluorescence Tomography to Detect Prostate Cancer

DTIC Science & Technology

2013-12-01

swept-source and a 20mm-diameter transverse-imaging intra-lumenal applicator with 7 source and 8 detector channels placed in a liquid phantom. Higher...3. RESULTS ON PHANTOM IMAGING The performance of this system configuration is evaluated by using liquid and solid phantoms. 3.1 Experiments setup... direc - tions. For each possible future location of the detector, the photon fluence rate at that position is compared with the case in the semi

Multimodal adaptive optics for depth-enhanced high-resolution ophthalmic imaging

NASA Astrophysics Data System (ADS)

Hammer, Daniel X.; Mujat, Mircea; Iftimia, Nicusor V.; Lue, Niyom; Ferguson, R. Daniel

2010-02-01

We developed a multimodal adaptive optics (AO) retinal imager for diagnosis of retinal diseases, including glaucoma, diabetic retinopathy (DR), age-related macular degeneration (AMD), and retinitis pigmentosa (RP). The development represents the first ever high performance AO system constructed that combines AO-corrected scanning laser ophthalmoscopy (SLO) and swept source Fourier domain optical coherence tomography (SSOCT) imaging modes in a single compact clinical prototype platform. The SSOCT channel operates at a wavelength of 1 μm for increased penetration and visualization of the choriocapillaris and choroid, sites of major disease activity for DR and wet AMD. The system is designed to operate on a broad clinical population with a dual deformable mirror (DM) configuration that allows simultaneous low- and high-order aberration correction. The system also includes a wide field line scanning ophthalmoscope (LSO) for initial screening, target identification, and global orientation; an integrated retinal tracker (RT) to stabilize the SLO, OCT, and LSO imaging fields in the presence of rotational eye motion; and a high-resolution LCD-based fixation target for presentation to the subject of stimuli and other visual cues. The system was tested in a limited number of human subjects without retinal disease for performance optimization and validation. The system was able to resolve and quantify cone photoreceptors across the macula to within ~0.5 deg (~100-150 μm) of the fovea, image and delineate ten retinal layers, and penetrate to resolve targets deep into the choroid. In addition to instrument hardware development, analysis algorithms were developed for efficient information extraction from clinical imaging sessions, with functionality including automated image registration, photoreceptor counting, strip and montage stitching, and segmentation. The system provides clinicians and researchers with high-resolution, high performance adaptive optics imaging to help guide therapies, develop new drugs, and improve patient outcomes.

Multimodal ophthalmic imaging using handheld spectrally encoded coherence tomography and reflectometry (SECTR)

NASA Astrophysics Data System (ADS)

Leeburg, Kelsey C.; El-Haddad, Mohamed T.; Malone, Joseph D.; Terrones, Benjamin D.; Tao, Yuankai K.

2018-02-01

Scanning laser ophthalmoscopy (SLO) provides high-speed, noninvasive en face imaging of the retinal fundus. Optical coherence tomography (OCT) is the current "gold-standard" for ophthalmic diagnostic imaging and enables depth-resolved visualization of ophthalmic structures and image-based surrogate biomarkers of disease. We present a compact optical and mechanical design for handheld spectrally encoded coherence tomography and reflectometry (SECTR) for multimodality en face spectrally encoded reflectometry (SER) and cross-sectional OCT imaging. We custom-designed a double-pass telecentric scan lens, which halves the size of 4-f optical relays and allowed us to reduce the footprint of our SECTR scan-head by a factor of >2.7x (volume) over our previous design. The double-pass scan lens was optimized for diffraction-limited performance over a +/-10° scan field. SECTR optics and optomechanics were combined in a compact rapid-prototyped enclosure with dimensions 87 x 141.8 x 137 mm (w x h x d). SECTR was implemented using a custom-built 400 kHz 1050 nm swept-source. OCT and SER were simultaneously digitized on dual input channels of a 4 GS/s digitizer at 1.4 GS/s per channel. In vivo human en face SER and cross-sectional OCT images were acquired at 350 fps. OCT volumes of 1000 B-scans were acquired in 2.86 s. We believe clinical translation of our compact handheld design will benefit point-of-care ophthalmic diagnostics in patients who are unable to be imaged on conventional slit-lamp based systems, such as infants and the bedridden. When combined with multi-volumetric registration methods, handheld SECTR will have advantages in motion-artifact free imaging over existing handheld technologies.

Scraping and stapling of end-grafted DNA chains by a bioadhesive spreading vesicle to reveal chain internal friction and topological complexity.

PubMed

Nam, Gimoon; Hisette, Marie Laure; Sun, Yuting Liang; Gisler, Thomas; Johner, Albert; Thalmann, Fabrice; Schröder, André Pierre; Marques, Carlos Manuel; Lee, Nam-Kyung

2010-08-20

Stained end-grafted DNA molecules about 20 μm long are scraped away and stretched out by the spreading front of a bioadhesive vesicle. Tethered biotin ligands bind the vesicle bilayer to a streptavidin substrate, stapling the DNAs into frozen confinement paths. Image analysis of the stapled DNA gives access, within optical resolution, to the local stretching values of individual DNA molecules swept by the spreading front, and provides evidence of self-entanglements.

Swept Impact Seismic Technique (SIST)

USGS Publications Warehouse

Park, C.B.; Miller, R.D.; Steeples, D.W.; Black, R.A.

1996-01-01

A coded seismic technique is developed that can result in a higher signal-to-noise ratio than a conventional single-pulse method does. The technique is cost-effective and time-efficient and therefore well suited for shallow-reflection surveys where high resolution and cost-effectiveness are critical. A low-power impact source transmits a few to several hundred high-frequency broad-band seismic pulses during several seconds of recording time according to a deterministic coding scheme. The coding scheme consists of a time-encoded impact sequence in which the rate of impact (cycles/s) changes linearly with time providing a broad range of impact rates. Impact times used during the decoding process are recorded on one channel of the seismograph. The coding concept combines the vibroseis swept-frequency and the Mini-Sosie random impact concepts. The swept-frequency concept greatly improves the suppression of correlation noise with much fewer impacts than normally used in the Mini-Sosie technique. The impact concept makes the technique simple and efficient in generating high-resolution seismic data especially in the presence of noise. The transfer function of the impact sequence simulates a low-cut filter with the cutoff frequency the same as the lowest impact rate. This property can be used to attenuate low-frequency ground-roll noise without using an analog low-cut filter or a spatial source (or receiver) array as is necessary with a conventional single-pulse method. Because of the discontinuous coding scheme, the decoding process is accomplished by a "shift-and-stacking" method that is much simpler and quicker than cross-correlation. The simplicity of the coding allows the mechanical design of the source to remain simple. Several different types of mechanical systems could be adapted to generate a linear impact sweep. In addition, the simplicity of the coding also allows the technique to be used with conventional acquisition systems, with only minor modifications.

Investigation at Low Speeds of Deflectors and Spoilers as Gust Alleviators on a Model of the Bell X-5 Airplane with 35 Degree Swept Wings and on a High-aspect-ratio 35 Degree Swept-wing-fuselage Model

NASA Technical Reports Server (NTRS)

Croom, Delwin R; Huffman, Jarrett K

1957-01-01

Results of an investigation at low speeds to determine the gust-alleviation capabilities (reduction in lift-curve slope) of spoilers and deflectors on a 35 degree swept-wing model of high aspect ratio and on a 1/4-scale model of the X-5 airplane with 35 degree swept wings indicate that deflector and spoiler-deflector types of controls can be designed to provide considerable gust alleviation for a swept-wing airplane while still maintaining stability and control.

Method and apparatus for modeling interactions

DOEpatents

Xavier, Patrick G.

2000-08-08

A method and apparatus for modeling interactions between bodies. The method comprises representing two bodies undergoing translations and rotations by two hierarchical swept volume representations. Interactions such as nearest approach and collision can be modeled based on the swept body representations. The present invention can serve as a practical tool in motion planning, CAD systems, simulation systems, safety analysis, and applications that require modeling time-based interactions. A body can be represented in the present invention by a union of convex polygons and convex polyhedra. As used generally herein, polyhedron includes polygon, and polyhedra includes polygons. The body undergoing translation can be represented by a swept body representation, where the swept body representation comprises a hierarchical bounding volume representation whose leaves each contain a representation of the region swept by a section of the body during the translation, and where the union of the regions is a superset of the region swept by the surface of the body during translation. Interactions between two bodies thus represented can be modeled by modeling interactions between the convex hulls of the finite sets of discrete points in the swept body representations.

Study of the feasibility aspects of flight testing an aeroelastically tailored forward swept research wing on a BQM-34F drone vehicle

NASA Technical Reports Server (NTRS)

Mourey, D. J.

1979-01-01

The aspects of flight testing an aeroelastically tailored forward swept research wing on a BQM-34F drone vehicle are examined. The geometry of a forward swept wing, which is incorporated into the BQM-34F to maintain satisfactory flight performance, stability, and control is defined. A preliminary design of the aeroelastically tailored forward swept wing is presented.

Non-contact rapid optical coherence elastography by high-speed 4D imaging of elastic waves

NASA Astrophysics Data System (ADS)

Song, Shaozhen; Yoon, Soon Joon; Ambroziński, Łukasz; Pelivanov, Ivan; Li, David; Gao, Liang; Shen, Tueng T.; O'Donnell, Matthew; Wang, Ruikang K.

2017-02-01

Shear wave OCE (SW-OCE) uses an OCT system to track propagating mechanical waves, providing the information needed to map the elasticity of the target sample. In this study we demonstrate high speed, 4D imaging to capture transient mechanical wave propagation. Using a high-speed Fourier domain mode-locked (FDML) swept-source OCT (SS-OCT) system operating at 1.62 MHz A-line rate, the equivalent volume rate of mechanical wave imaging is 16 kvps (kilo-volumes per second), and total imaging time for a 6 x 6 x 3 mm volume is only 0.32 s. With a displacement sensitivity of 10 nanometers, the proposed 4D imaging technique provides sufficient temporal and spatial resolution for real-time optical coherence elastography (OCE). Combined with a new air-coupled, high-frequency focused ultrasound stimulator requiring no contact or coupling media, this near real-time system can provide quantitative information on localized viscoelastic properties. SW-OCE measurements are demonstrated on tissue-mimicking phantoms and porcine cornea under various intra-ocular pressures. In addition, elasticity anisotropy in the cornea is observed. Images of the mechanical wave group velocity, which correlates with tissue elasticity, show velocities ranging from 4-20 m/s depending on pressure and propagation direction. These initial results strong suggest that 4D imaging for real-time OCE may enable high-resolution quantitative mapping of tissue biomechanical properties in clinical applications.

Influence of dental resin material composition on cross-polarization-optical coherence tomography imaging

PubMed Central

Lammeier, Carmen; Li, YuPing; Lunos, Scott; Fok, Alex; Rudney, Joel

2012-01-01

Abstract. The purpose of this study was to investigate cross-polarization-optical coherence tomography (CP-OCT) signal attenuation through different resin material compositions. Four distinct composite systems were used: Filtek supreme ultra (FSU) (3M ESPE), IPS empress direct (EMD) (Ivoclar Vivadent), estelite sigma quick (SQK) (Tokuyama Dental), and Z100 (3M ESPE). Cross-sectional images of different composite-demineralized phantoms (n=108) were collected using a 1310-nm intraoral cross-polarization swept source OCT (CP-OCT) imaging system. %T quantified the CP-OCT signal attenuation. Scanning electron microscopy, transmission electron microscopy, and energy-dispersive x-ray spectrometer chemical analysis was utilized to determine how different matrix/filler compositions affected attenuation of the near infrared (NIR) signal. CP-OCT imaging of dental resin composites showed enormous variation in signal attenuation. For each of our composite systems, there was not a consistent attenuation difference in the NIR signal for A to D shades. The four composites had similar measured backscattering values but attenuated the overall signal to different degrees. When comparing the A2 shades between the four different composite systems, the order of highest to lowest of %T was EMD>Z100, FSU>SQK (ANOVA, Tukey, p<0.0001). As a result, we demonstrate the importance of understanding how the constituents of composite materials affect CP-OCT signal attenuation. PMID:23224001

Volumetric vessel reconstruction method for absolute blood flow velocity measurement in Doppler OCT images

NASA Astrophysics Data System (ADS)

Qi, Li; Zhu, Jiang; Hancock, Aneeka M.; Dai, Cuixia; Zhang, Xuping; Frostig, Ron D.; Chen, Zhongping

2017-02-01

Doppler optical coherence tomography (DOCT) is considered one of the most promising functional imaging modalities for neuro biology research and has demonstrated the ability to quantify cerebral blood flow velocity at a high accuracy. However, the measurement of total absolute blood flow velocity (BFV) of major cerebral arteries is still a difficult problem since it not only relates to the properties of the laser and the scattering particles, but also relates to the geometry of both directions of the laser beam and the flow. In this paper, focusing on the analysis of cerebral hemodynamics, we presents a method to quantify the total absolute blood flow velocity in middle cerebral artery (MCA) based on volumetric vessel reconstruction from pure DOCT images. A modified region growing segmentation method is first used to localize the MCA on successive DOCT B-scan images. Vessel skeletonization, followed by an averaging gradient angle calculation method, is then carried out to obtain Doppler angles along the entire MCA. Once the Doppler angles are determined, the absolute blood flow velocity of each position on the MCA is easily found. Given a seed point position on the MCA, our approach could achieve automatic quantification of the fully distributed absolute BFV. Based on experiments conducted using a swept-source optical coherence tomography system, our approach could achieve automatic quantification of the fully distributed absolute BFV across different vessel branches in the rodent brain.

Quantifying the effects of hydration on corneal stiffness with optical coherence elastography

NASA Astrophysics Data System (ADS)

Singh, Manmohan; Li, Jiasong; Han, Zhaolong; Vantipalli, Srilatha; Aglyamov, Salavat R.; Twa, Michael D.; Larin, Kirill V.

2018-02-01

Several methods have been proposed to assess changes in corneal biomechanical properties due to various factors, such as degenerative diseases, intraocular pressure, and therapeutic interventions (e.g. corneal collagen crosslinking). However, the effect of the corneal tissue hydration state on corneal stiffness is not well understood. In this work, we induce low amplitude (< 10 μm) elastic waves with a focused micro air-pulse in fresh in situ rabbit corneas (n = 10) in the whole eye-globe configuration at an artificially controlled intraocular pressure. The waves were then detected with a phase-stabilized swept source optical coherence elastography system. Baseline measurements were taken every 20 minutes for an hour while the corneas were hydrated with 1X PBS. After the measurement at 60 minutes, a 20% dextran solution was topically instilled to dehydrate the corneas. The measurements were repeated every 20 minutes again for an hour. The results showed that the elastic wave velocity decreased as the corneal thickness decreased. Finite element modeling (FEM) was performed using the corneal geometry and elastic wave propagation speed to assess the stiffness of the samples. The results show that the stiffness increased from 430 kPa during hydration with PBS to 500 kPa after dehydration with dextran, demonstrating that corneal hydration state, apart from geometry and intraocular pressure, can change the stiffness of the cornea.

Optical coherence tomography imaging of colonic crypts in a mouse model of colorectal cancer

NASA Astrophysics Data System (ADS)

Welge, Weston A.; Barton, Jennifer K.

2016-03-01

Aberrant crypt foci (ACF) are abnormal epithelial lesions that precede development of colonic polyps. As the earliest morphological change in the development of colorectal cancer, ACF is a highly studied phenomenon. The most common method of imaging ACF is chromoendoscopy using methylene blue as a contrast agent. Narrow- band imaging is a contrast-agent-free modality for imaging the colonic crypts. Optical coherence tomography (OCT) is an attractive alternative to chromoendoscopy and narrow-band imaging because it can resolve the crypt structure at sufficiently high sampling while simultaneously providing depth-resolved data. We imaged in vivo the distal 15 mm of colon in the azoxymethane (AOM) mouse model of colorectal cancer using a commercial swept-source OCT system and a miniature endoscope designed and built in-house. We present en face images of the colonic crypts and demonstrate that different patterns in healthy and adenoma tissue can be seen. These patterns correspond to those reported in the literature. We have previously demonstrated early detection of colon adenoma using OCT by detecting minute thickening of the mucosa. By combining mucosal thickness measurement with imaging of the crypt structure, OCT can be used to correlate ACF and adenoma development in space and time. These results suggest that OCT may be a superior imaging modality for studying the connection between ACF and colorectal cancer.

Microscope-Integrated Optical Coherence Tomography Angiography in the Operating Room in Young Children With Retinal Vascular Disease.

PubMed

Chen, Xi; Viehland, Christian; Carrasco-Zevallos, Oscar M; Keller, Brenton; Vajzovic, Lejla; Izatt, Joseph A; Toth, Cynthia A

2017-05-01

Intraoperative optical coherence tomography (OCT) has gained traction as an important adjunct for clinical decision making during vitreoretinal surgery, and OCT angiography (OCTA) has provided novel insights in clinical evaluation of retinal diseases. To date, these two technologies have not been applied in combination to evaluate retinal vascular disease in the operating suite. To conduct microscope-integrated, swept-source OCTA (MIOCTA) in children with retinal vascular disease. In this case report analysis, OCT imaging in pediatric patients, MIOCTA images were obtained during examination under anesthesia from a young boy with a history of idiopathic vitreous hemorrhage and a female infant with familial exudative vitreoretinopathy. Side-by-side comparison of research MIOCT angiograms and clinically indicated fluorescein angiograms. In 2 young children with retinal vascular disease, the MIOCTA images showed more detailed vascular patterns than were visible on the fluorescein angiograms although within a more posterior field of view. The MIOCTA system allowed visualization of small pathological retinal vessels in the retinal periphery that were obscured in the fluorescein angiograms by fluorescein staining from underlying, preexisting laser scars. This is the first report to date of the use of MIOCTA in the operating room for young children with retinal vascular disease. Further optimization of this system may allow noninvasive detailed evaluation of retinal vasculature during surgical procedures and in patients who could not cooperate with in-office examinations.

Agreement between Gonioscopic Examination and Swept Source Fourier Domain Anterior Segment Optical Coherence Tomography Imaging

PubMed Central

Nguyen, Donna; Minnal, Vandana R.

2016-01-01

Purpose. To evaluate interobserver, intervisit, and interinstrument agreements for gonioscopy and Fourier domain anterior segment optical coherence tomography (FD ASOCT) for classifying open and narrow angle eyes. Methods. Eighty-six eyes with open or narrow anterior chamber angles were included. The superior angle was classified open or narrow by 2 of 5 glaucoma specialists using gonioscopy and imaged by FD ASOCT in the dark. The superior angle of each FD ASOCT image was graded as open or narrow by 2 masked readers. The same procedures were repeated within 6 months. Kappas for interobserver and intervisit agreements for each instrument and interinstrument agreements were calculated. Results. The mean age was 50.9 (±18.4) years. Interobserver agreements were moderate to good for both gonioscopy (0.57 and 0.69) and FD ASOCT (0.58 and 0.75). Intervisit agreements were moderate to excellent for both gonioscopy (0.53 to 0.86) and FD ASOCT (0.57 and 0.85). Interinstrument agreements were fair to good (0.34 to 0.63), with FD ASOCT classifying more angles as narrow than gonioscopy. Conclusions. Both gonioscopy and FD ASOCT examiners were internally consistent with similar interobserver and intervisit agreements for angle classification. Agreement between instruments was fair to good, with FD ASOCT classifying more angles as narrow than gonioscopy. PMID:27990300

Automated 3-D cell counting method for grading uveitis of rodent eye in vivo with optical coherence tomograph.

PubMed

Choi, Woo June; Pepple, Kathryn L; Wang, Ruikang K

2018-05-24

In preclinical vision research, cell grading in small animal models is essential for the quantitative evaluation of intraocular inflammation. Here, we present a new and practical optical coherence tomography (OCT) image analysis method for the automated detection and counting of aqueous cells in the anterior chamber (AC) of a rodent model of uveitis. Anterior segment OCT (AS-OCT) images are acquired with a 100kHz swept-source OCT (SS-OCT) system. The proposed method consists of two steps. In the first step, we first despeckle and binarize each OCT image. After removing AS structures in the binary image, we then apply area thresholding to isolate cell-like objects. Potential cell candidates are selected based on their best fit to roundness. The second step performs the cell counting within the whole AC, in which additional cell tracking analysis is conducted on the successive OCT images to eliminate redundancy in cell counting. Finally, 3-D cell grading using the proposed method is demonstrated in longitudinal OCT imaging of a mouse model of anterior uveitis in vivo. Rendering of anterior segment (orange) of mouse eye and automatically counted anterior chamber cells (green). Inset is a top view of the rendering, showing the cell distribution across the anterior chamber. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Influence of dental resin material composition on cross-polarization-optical coherence tomography imaging

NASA Astrophysics Data System (ADS)

Lammeier, Carmen; Li, YuPing; Lunos, Scott; Fok, Alex; Rudney, Joel; Jones, Robert S.

2012-10-01

The purpose of this study was to investigate cross-polarization-optical coherence tomography (CP-OCT) signal attenuation through different resin material compositions. Four distinct composite systems were used: Filtek supreme ultra (FSU) (3M ESPE), IPS empress direct (EMD) (Ivoclar Vivadent), estelite sigma quick (SQK) (Tokuyama Dental), and Z100 (3M ESPE). Cross-sectional images of different composite-demineralized phantoms (n=108) were collected using a 1310-nm intraoral cross-polarization swept source OCT (CP-OCT) imaging system. %T quantified the CP-OCT signal attenuation. Scanning electron microscopy, transmission electron microscopy, and energy-dispersive x-ray spectrometer chemical analysis was utilized to determine how different matrix/filler compositions affected attenuation of the near infrared (NIR) signal. CP-OCT imaging of dental resin composites showed enormous variation in signal attenuation. For each of our composite systems, there was not a consistent attenuation difference in the NIR signal for A to D shades. The four composites had similar measured backscattering values but attenuated the overall signal to different degrees. When comparing the A2 shades between the four different composite systems, the order of highest to lowest of %T was EMD>Z100, FSU>SQK (ANOVA, Tukey, p<0.0001). As a result, we demonstrate the importance of understanding how the constituents of composite materials affect CP-OCT signal attenuation.

In vivo endoscopic Doppler optical coherence tomography imaging of mouse colon

NASA Astrophysics Data System (ADS)

Welge, Weston A.; Barton, Jennifer K.

2016-03-01

Colorectal cancer remains the second deadliest cancer in the United States, despite the high sensitivity and specificity of colonoscopy and sigmoidoscopy. While these standard imaging procedures can accurately detect medium and large polyps, some studies have shown miss rates up to 25% for polyps less than 5 mm in diameter. An imaging modality capable of detecting small lesions could potentially improve patient outcomes. Optical coherence tomography (OCT) has been shown to be a powerful imaging modality for adenoma detection in a mouse model of colorectal cancer. While previous work has focused on analyzing the structural OCT images based on thickening of the mucosa and changes in light attenuation in depth, imaging the microvasculature of the colon may enable earlier detection of polyps. The structure and function of vessels grown to support tumor growth are markedly different from healthy vessels. Doppler OCT is capable of imaging microvessels in vivo. We developed a method of processing raw fringe data from a commercial swept-source OCT system using a lab-built miniature endoscope to extract microvessels. This method can be used to measure vessel count and density and to measure flow velocities. This may improve early detection and aid in the development of new chemopreventive and chemotherapeutic drugs. We present, to the best of our knowledge, the first endoscopic Doppler OCT images of in vivo mouse colon.

Non-Invasive Periodontal Probing Through Fourier-Domain Optical Coherence Tomography.

PubMed

Mota, Cláudia C B O; Fernandes, Luana O; Cimões, Renata; Gomes, Anderson S L

2015-09-01

Periodontitis is a multifactorial and infectious disease that may result in significant debilitation. The aim of this study is to exploit two optical coherence tomography (OCT) systems operating in the Fourier domain at different wavelengths, 930 and 1,325 nm, for structural analysis of periodontal tissue in porcine jaws. Five fresh porcine jaws were sectioned and stored in formalin before OCT analysis. Two- and three-dimensional OCT images of the tooth/gingiva interface were performed, and measurements of the gingival structures were obtained. The 930-nm OCT system operates in the spectral domain, whereas the 1,325-nm system is a swept-source model. Stereomicroscope images, the gold standard, were used for direct comparison. Through image analysis, it is possible to identify the free gingiva and the attached gingiva, the calculus deposition over tooth surfaces, and the subgingival calculus that enables the enlargement of the gingival sulcus. In addition, the gingival thickness and the gingival sulcus depth can be non-invasively measured, varying from 0.8 to 4 mm. Regarding the ability of the two OCT systems to visualize periodontal structures, the system operating at 1,325 nm shows a better performance, owing to a longer central wavelength that allows deeper tissue penetration. The results with the system at 930 nm can also be used, but some features could not be observed due to its lower penetration depth in the tissue.

Assessment of natural enamel lesions with optical coherence tomography in comparison with microfocus x-ray computed tomography.

PubMed

Espigares, Jorge; Sadr, Alireza; Hamba, Hidenori; Shimada, Yasushi; Otsuki, Masayuki; Tagami, Junji; Sumi, Yasunori

2015-01-01

A technology to characterize early enamel lesions is needed in dentistry. Optical coherence tomography (OCT) is a noninvasive method that provides high-resolution cross-sectional images. The aim of this study is to compare OCT with microfocus x-ray computed tomography ([Formula: see text]) for assessment of natural enamel lesions in vitro. Ten human teeth with visible white spot-like changes on the enamel smooth surface and no cavitation (ICDAS code 2) were subjected to imaging by μCT (SMX-100CT, Shimadzu) and 1300-nm swept-source OCT (Dental SS-OCT, Panasonic Health Care). In [Formula: see text], the lesions appeared as radiolucent dark areas, while in SS-OCT, they appeared as areas of increased signal intensity beneath the surface. An SS-OCT attenuation coefficient based on Beer-Lambert law could discriminate lesions from sound enamel. Lesion depth ranged from 175 to [Formula: see text] in SS-OCT. A correlation between [Formula: see text] and SS-OCT was found regarding lesion depth ([Formula: see text], [Formula: see text]) and also surface layer thickness ([Formula: see text], [Formula: see text]). The images obtained clinically in real time using the dental SS-OCT system are suitable for the assessment of natural subsurface lesions and their surface layer, providing comparable images to a laboratory high-resolution [Formula: see text] without the use of x-ray.

Assessment of natural enamel lesions with optical coherence tomography in comparison with microfocus x-ray computed tomography

PubMed Central

Espigares, Jorge; Sadr, Alireza; Hamba, Hidenori; Shimada, Yasushi; Otsuki, Masayuki; Tagami, Junji; Sumi, Yasunori

2015-01-01

Abstract. A technology to characterize early enamel lesions is needed in dentistry. Optical coherence tomography (OCT) is a noninvasive method that provides high-resolution cross-sectional images. The aim of this study is to compare OCT with microfocus x-ray computed tomography (μCT) for assessment of natural enamel lesions in vitro. Ten human teeth with visible white spot-like changes on the enamel smooth surface and no cavitation (ICDAS code 2) were subjected to imaging by μCT (SMX-100CT, Shimadzu) and 1300-nm swept-source OCT (Dental SS-OCT, Panasonic Health Care). In μCT, the lesions appeared as radiolucent dark areas, while in SS-OCT, they appeared as areas of increased signal intensity beneath the surface. An SS-OCT attenuation coefficient based on Beer–Lambert law could discriminate lesions from sound enamel. Lesion depth ranged from 175 to 606  μm in SS-OCT. A correlation between μCT and SS-OCT was found regarding lesion depth (R=0.81, p<0.001) and also surface layer thickness (R=0.76, p<0.005). The images obtained clinically in real time using the dental SS-OCT system are suitable for the assessment of natural subsurface lesions and their surface layer, providing comparable images to a laboratory high-resolution μCT without the use of x-ray. PMID:26158079

Comparative Study of Anterior Eye Segment Measurements with Spectral Swept-Source and Time-Domain Optical Coherence Tomography in Eyes with Corneal Dystrophies

PubMed Central

Teper, Sławomir J.; Janiszewska, Dominika A.; Lyssek-Boron, Anita; Dobrowolski, Dariusz; Koprowski, Robert; Wylegala, Edward

2015-01-01

Purpose. To compare anterior eye segment measurements and morphology obtained with two optical coherence tomography systems (TD OCT, SS OCT) in eyes with corneal dystrophies (CDs). Methods. Fifty healthy volunteers (50 eyes) and 54 patients (96 eyes) diagnosed with CD (epithelial basement membrane dystrophy, EBMD = 12 eyes; Thiel-Behnke CD = 6 eyes; lattice CD TGFBI type = 15 eyes; granular CD type 1 = 7 eyes, granular CD type 2 = 2 eyes; macular CD = 23 eyes; and Fuchs endothelial CD = 31 eyes) were recruited for the study. Automated and manual central corneal thickness (aCCT, mCCT), anterior chamber depth (ACD), and nasal and temporal trabecular iris angle (nTIA, tTIA) were measured and compared with Bland-Altman plots. Results. Good agreement between the TD and SS OCT measurements was demonstrated for mCCT and aCCT in normal individuals and for mCCT in the CDs group. The ACD, nTIA, and tTIA measurements differed significantly in both groups. TBCD, LCD, and FECD caused increased CCT. MCD caused significant corneal thinning. FECD affected all analyzed parameters. Conclusions. Better agreement between SS OCT and TD OCT measurements was demonstrated in normal individuals compared to the CDs group. OCT provides comprehensive corneal deposits analysis and demonstrates the association of CD with CCT, ACD, and TIA measurements. PMID:26457303

Comparative Study of Anterior Eye Segment Measurements with Spectral Swept-Source and Time-Domain Optical Coherence Tomography in Eyes with Corneal Dystrophies.

PubMed

Nowinska, Anna K; Teper, Sławomir J; Janiszewska, Dominika A; Lyssek-Boron, Anita; Dobrowolski, Dariusz; Koprowski, Robert; Wylegala, Edward

2015-01-01

To compare anterior eye segment measurements and morphology obtained with two optical coherence tomography systems (TD OCT, SS OCT) in eyes with corneal dystrophies (CDs). Fifty healthy volunteers (50 eyes) and 54 patients (96 eyes) diagnosed with CD (epithelial basement membrane dystrophy, EBMD = 12 eyes; Thiel-Behnke CD = 6 eyes; lattice CD TGFBI type = 15 eyes; granular CD type 1 = 7 eyes, granular CD type 2 = 2 eyes; macular CD = 23 eyes; and Fuchs endothelial CD = 31 eyes) were recruited for the study. Automated and manual central corneal thickness (aCCT, mCCT), anterior chamber depth (ACD), and nasal and temporal trabecular iris angle (nTIA, tTIA) were measured and compared with Bland-Altman plots. Good agreement between the TD and SS OCT measurements was demonstrated for mCCT and aCCT in normal individuals and for mCCT in the CDs group. The ACD, nTIA, and tTIA measurements differed significantly in both groups. TBCD, LCD, and FECD caused increased CCT. MCD caused significant corneal thinning. FECD affected all analyzed parameters. Better agreement between SS OCT and TD OCT measurements was demonstrated in normal individuals compared to the CDs group. OCT provides comprehensive corneal deposits analysis and demonstrates the association of CD with CCT, ACD, and TIA measurements.

Different foveal schisis patterns in each retinal layer in eyes with hereditary juvenile retinoschisis evaluated by en-face optical coherence tomography.

PubMed

Yoshida-Uemura, Tomoyo; Katagiri, Satoshi; Yokoi, Tadashi; Nishina, Sachiko; Azuma, Noriyuki

2017-04-01

To analyze the structures of schisis in eyes with hereditary juvenile retinoschisis using en-face optical coherence tomography (OCT) imaging. In this retrospective observational study, we reviewed the medical records of patients with hereditary juvenile retinoschisis who underwent comprehensive ophthalmic examinations including swept-source OCT. OCT images were obtained from 16 eyes of nine boys (mean age ± standard deviation, 10.6 ± 4.0 years). The horizontal OCT images at the fovea showed inner nuclear layer (INL) schisis in one eye (6.3 %), ganglion cell layer (GCL) and INL schisis in 12 eyes (75.0 %), INL and outer plexiform layer (OPL) schisis in two eyes (12.5 %), and GCL, INL, and OPL schisis in one eye (6.3 %). En-face OCT images showed characteristic schisis patterns in each retinal layer, which were represented by multiple hyporeflective holes in the parafoveal region in the GCL, a spoke-like pattern in the foveal region, a reticular pattern in the parafoveal region in the INL, and multiple hyporeflective polygonal cavities with partitions in the OPL. Our results using en-face OCT imaging clarified different patterns of schisis formation among the GCL, INL, and OPL, which lead to further recognition of structure in hereditary juvenile retinoschisis.

Wavelength-Modulated Differential Photoacoustic Spectroscopy (WM-DPAS): Theory of a High-Sensitivity Methodology for the Detection of Early-Stage Tumors in Tissues

NASA Astrophysics Data System (ADS)

Choi, S.; Mandelis, A.; Guo, X.; Lashkari, B.; Kellnberger, S.; Ntziachristos, V.

2015-06-01

In the field of medical diagnostics, biomedical photoacoustics (PA) is a non-invasive hybrid optical-ultrasonic imaging modality. Due to the unique hybrid capability of optical and acoustic imaging, PA imaging has risen to the frontiers of medical diagnostic procedures such as human breast cancer detection. While conventional PA imaging has been mainly carried out by a high-power pulsed laser, an alternative technology, the frequency domain biophotoacoustic radar (FD-PAR) is under intensive development. It utilizes a continuous wave optical source with the laser intensity modulated by a frequency-swept waveform for acoustic wave generation. The small amplitude of the generated acoustic wave is significantly compensated by increased signal-to-noise ratio (several orders of magnitude) using matched-filter and pulse compression correlation processing in a manner similar to radar systems. The current study introduces the theory of a novel FD-PAR modality for ultra-sensitive characterization of functional information for breast cancer imaging. The newly developed theory of wavelength-modulated differential PA spectroscopy (WM-DPAS) detection has been introduced to address angiogenesis and hypoxia monitoring, two well-known benchmarks of breast tumor formation. Based on the WM-DPAS theory, this modality efficiently suppresses background absorptions and is expected to detect very small changes in total hemoglobin concentration and oxygenation levels, thereby identifying pre-malignant tumors before they are anatomically apparent. An experimental system design for the WM-DPAS is presented and preliminary single-ended laser experimental results were obtained and compared to a limiting case of the developed theoretical formalism.

The effect of internal fixation lamp on anterior chamber angle width measured by anterior segment optical coherence tomography.

PubMed

Nakamine, Sakari; Sakai, Hiroshi; Arakaki, Yoshikuni; Yonahara, Michiko; Kaiya, Tadayoshi

2018-01-01

To study the effect of the internal fixation lamp on anterior chamber width measured by anterior segment optical coherence tomography. In a prospective cross sectional observational study, consecutive 22 right eyes of 22 patients (4 men and 18 women) with suspected primary angle closure underwent swept source domain anterior segment optical coherence tomography (AS-OCT), (CASIA SS-1000, Tomey, Nagoya, Japan). Anterior chamber parameters of angle opening distance (AOD), trabecular-iris angle (TIA), angle recess area (ARA) at 500 or 750 µm from scleral spur and pupil diameter were measured by AS-OCT in a three-dimensional mode in 4 quadrants (superior, inferior, temporal and nasal) in dark room setting both with and without internal fixation lamp. Anterior segment parameters of AOD 500 in superior, inferior and temporal quadrants, AOD 750 at superior and nasal, TIA 500 at superior, and inferior and TIA 750 at superior and nasal, and ARA 500 or 750 at superior and inferior with internal fixation lamp were greater and the pupil diameter was significantly (all P < 0.05, paired t test) smaller than when measured without fixation lamp. Internal fixation lamp of the anterior segment OCT makes the pupil constrict and angle wider. When using AS-OCT with usual setting with internal fixation lamp on with eyes in which the anterior chamber angle is narrow but open, it is recommended that the internal fixation lamp be turned off to ensure a clear indication as to whether the angle is open or closed in the dark.

Parametric spectro-temporal analyzer (PASTA) for ultrafast optical performance monitoring

NASA Astrophysics Data System (ADS)

Zhang, Chi; Wong, Kenneth K. Y.

2013-12-01

Ultrafast optical spectrum monitoring is one of the most challenging tasks in observing ultrafast phenomena, such as the spectroscopy, dynamic observation of the laser cavity, and spectral encoded imaging systems. However, conventional method such as optical spectrum analyzer (OSA) spatially disperses the spectrum, but the space-to-time mapping is realized by mechanical rotation of a grating, so are incapable of operating at high speed. Besides the spatial dispersion, temporal dispersion provided by dispersive fiber can also stretches the spectrum in time domain in an ultrafast manner, but is primarily confined in measuring short pulses. In view of these constraints, here we present a real-time spectrum analyzer called parametric spectro-temporal analyzer (PASTA), which is based on the time-lens focusing mechanism. It achieves a 100-MHz frame rate and can measure arbitrary waveforms. For the first time, we observe the dynamic spectrum of an ultrafast swept-source: Fourier domain mode-locked (FDML) laser, and the spectrum evolution of a laser cavity during its stabilizing process. In addition to the basic single-lens structure, the multi-lens configurations (e.g. telescope or wide-angle scope) will provide a versatile operating condition, which can zoom in to achieve 0.05-nm resolution and zoom out to achieve 10-nm observation range, namely 17 times zoom in/out ratio. In view of the goal of achieving spectrum analysis with fine accuracy, PASTA provides a promising path to study the real-time spectrum of some dynamic phenomena and non-repetitive events, with orders of magnitude enhancement in the frame rate over conventional OSAs.

Dynamic phase-sensitive optical coherence elastography at a true kilohertz frame-rate

NASA Astrophysics Data System (ADS)

Singh, Manmohan; Wu, Chen; Liu, Chih-Hao; Li, Jiasong; Schill, Alexander; Nair, Achuth; Larin, Kirill V.

2016-03-01

Dynamic optical coherence elastography (OCE) techniques have rapidly emerged as a noninvasive way to characterize the biomechanical properties of tissue. However, clinical applications of the majority of these techniques have been unfeasible due to the extended acquisition time because of multiple temporal OCT acquisitions (M-B mode). Moreover, multiple excitations, large datasets, and prolonged laser exposure prohibit their translation to the clinic, where patient discomfort and safety are critical criteria. Here, we demonstrate the feasibility of noncontact true kilohertz frame-rate dynamic optical coherence elastography by directly imaging a focused air-pulse induced elastic wave with a home-built phase-sensitive OCE system. The OCE system was based on a 4X buffered Fourier Domain Mode Locked swept source laser with an A-scan rate of ~1.5 MHz, and imaged the elastic wave propagation at a frame rate of ~7.3 kHz. Because the elastic wave directly imaged, only a single excitation was utilized for one line scan measurement. Rather than acquiring multiple temporal scans at successive spatial locations as with previous techniques, here, successive B-scans were acquired over the measurement region (B-M mode). Preliminary measurements were taken on tissue-mimicking agar phantoms of various concentrations, and the results showed good agreement with uniaxial mechanical compression testing. Then, the elasticity of an in situ porcine cornea in the whole eye-globe configuration at various intraocular pressures was measured. The results showed that this technique can acquire a depth-resolved elastogram in milliseconds. Furthermore, the ultra-fast acquisition ensured that the laser safety exposure limit for the cornea was not exceeded.

Nerve Fiber Flux Analysis Using Wide-Field Swept-Source Optical Coherence Tomography.

PubMed

Tan, Ou; Liu, Liang; Liu, Li; Huang, David

2018-02-01

To devise a method to quantify nerve fibers over their arcuate courses over an extended peripapillary area using optical coherence tomography (OCT). Participants were imaged with 8 × 8-mm volumetric OCT scans centered at the optic disc. A new quantity, nerve fiber flux (NFF), represents the cross-sectional area transected perpendicular to the nerve fibers. The peripapillary area was divided into 64 tracks with equal flux. An iterative algorithm traced the trajectory of the tracks assuming that the relative distribution of the NFF was conserved with compensation for fiber connections to ganglion cells on the macular side. Average trajectory was averaged from normal eyes and use to calculate the NFF maps for glaucomatous eyes. The NFF maps were divided into eight sectors that correspond to visual field regions. There were 24 healthy and 10 glaucomatous eyes enrolled. The algorithm converged on similar patterns of NFL tracks for all healthy eyes. In glaucomatous eyes, NFF correlated with visual field sensitivity in the arcuate sectors (Spearman ρ = 0.53-0.62). Focal nerve fiber loss in glaucomatous eyes appeared as uniform tracks of NFF defects that followed the expected arcuate fiber trajectory. Using an algorithm based on the conservation of flux, we derived nerve fiber trajectories in the peripapillary area. The NFF map is useful for the visualization of focal defects and quantification of sector nerve fiber loss from wide-area volumetric OCT scans. NFF provides a cumulative measure of volumetric loss along nerve fiber tracks and could improve the detection of focal glaucoma damage.

Loading an Optical Trap with Diamond Nanocrystals Containing Nitrogen-Vacancy Centers from a Surface

NASA Astrophysics Data System (ADS)

Hsu, Jen-Feng; Ji, Peng; Dutt, M. V. Gurudev; D'Urso, Brian R.

2015-03-01

We present a simple and effective method of loading particles into an optical trap. Our primary application of this method is loading photoluminescent material, such as diamond nanocrystals containing nitrogen-vacancy (NV) centers, for coupling the mechanical motion of the trapped crystal with the spin of the NV centers. Highly absorptive material at the trapping laser frequency, such as tartrazine dye, is used as media to attach nanodiamonds and burn into a cloud of air-borne particles as the material is swept near the trapping laser focus on a glass slide. Particles are then trapped with the laser used for burning or transferred to a second laser trap at a different wavelength. Evidence of successful loading diamond nanocrystals into the trap presented includes high sensitivity of the photoluminecscence (PL) to the excitation laser and the PL spectra of the optically trapped particles

Ultra-widefield retinal MHz-OCT imaging with up to 100 degrees viewing angle.

PubMed

Kolb, Jan Philip; Klein, Thomas; Kufner, Corinna L; Wieser, Wolfgang; Neubauer, Aljoscha S; Huber, Robert

2015-05-01

We evaluate strategies to maximize the field of view (FOV) of in vivo retinal OCT imaging of human eyes. Three imaging modes are tested: Single volume imaging with 85° FOV as well as with 100° and stitching of five 60° images to a 100° mosaic (measured from the nodal point). We employ a MHz-OCT system based on a 1060nm Fourier domain mode locked (FDML) laser with a depth scan rate of 1.68MHz. The high speed is essential for dense isotropic sampling of the large areas. Challenges caused by the wide FOV are discussed and solutions to most issues are presented. Detailed information on the design and characterization of our sample arm optics is given. We investigate the origin of an angle dependent signal fall-off which we observe towards larger imaging angles. It is present in our 85° and 100° single volume images, but not in the mosaic. Our results suggest that 100° FOV OCT is possible with current swept source OCT technology.

Ultra-widefield retinal MHz-OCT imaging with up to 100 degrees viewing angle

PubMed Central

Kolb, Jan Philip; Klein, Thomas; Kufner, Corinna L.; Wieser, Wolfgang; Neubauer, Aljoscha S.; Huber, Robert

2015-01-01

We evaluate strategies to maximize the field of view (FOV) of in vivo retinal OCT imaging of human eyes. Three imaging modes are tested: Single volume imaging with 85° FOV as well as with 100° and stitching of five 60° images to a 100° mosaic (measured from the nodal point). We employ a MHz-OCT system based on a 1060nm Fourier domain mode locked (FDML) laser with a depth scan rate of 1.68MHz. The high speed is essential for dense isotropic sampling of the large areas. Challenges caused by the wide FOV are discussed and solutions to most issues are presented. Detailed information on the design and characterization of our sample arm optics is given. We investigate the origin of an angle dependent signal fall-off which we observe towards larger imaging angles. It is present in our 85° and 100° single volume images, but not in the mosaic. Our results suggest that 100° FOV OCT is possible with current swept source OCT technology. PMID:26137363

Update on visual function and choroidal-retinal thickness alterations in Parkinson's disease.

PubMed

Obis, J; Satue, M; Alarcia, R; Pablo, L E; Garcia-Martin, E

2018-05-01

Parkinson's disease (PD) is a neurodegenerative process that affects 7.5 million people around the world. Since 2004, several studies have demonstrated changes in various retinal layers in PD using optical coherence tomography (OCT). However, there are some discrepancies in the results of those studies. Some of them have correlated retinal thickness with the severity or duration of the disease, demonstrating that OCT measurements may be an innocuous and easy biomarker for PD progression. Other studies have demonstrated visual dysfunctions since early phases of the disease. Lastly, the most recent studies that use Swept Source OCT technology, have found choroidal thickness increase in PD patients and provide new information related to the retinal degenerative process in this disease. The aim of this paper is to review the literature on OCT and PD, in order to determine the altered retinal and choroidal parameters in PD and their possible clinical usefulness, and also the visual dysfunctions with higher impact in these patients. Copyright © 2018 Sociedad Española de Oftalmología. Publicado por Elsevier España, S.L.U. All rights reserved.

Blade-to-Blade Variations in Shocks Upstream of Both a Forward-Swept and an Aft-Swept Fan

NASA Technical Reports Server (NTRS)

Podboy, Gary G.; Krupar, Martin J.

2006-01-01

Detailed laser Doppler velocimeter (LDV) flow field measurements were made upstream of two fans, one forward-swept and one aft-swept, in order to learn more about the shocks which propagate upstream of these rotors when they are operated at supersonic tip speeds. The blade-to-blade variations in the flows associated with these shocks are thought to be responsible for generating Multiple Pure Tone (MPT) noise. The measured blade-to-blade variations are documented in this report through a series of slideshows which show relative Mach number contours computed from the velocity measurements. Data are presented for the forward-swept fan operating at three speeds (corresponding to tip relative Mach numbers of 0.817, 1.074, and 1.189), and for the aft-swept fan operating at two (tip relative Mach numbers of 1.074 and 1.189). These LDV data illustrate how the perturbations in the upstream flow field created by the rotating blades vary with axial position, radial position and rotor speed. As expected, at the highest tested speed the forward-swept fan swallowed the shocks which occur in the tip region, whereas the aftswept fan did not. This resulted in a much smaller flow disturbance just upstream of the tip of the forward-swept fan. Nevertheless, further upstream the two fan flows were much more similar.

Widely tunable quantum cascade laser-based terahertz source.

PubMed

Danylov, Andriy A; Light, Alexander R; Waldman, Jerry; Erickson, Neal; Qian, Xifeng

2014-07-10

A compact, tunable, ultranarrowband terahertz source, Δν∼1  MHz, is demonstrated by upconversion of a 2.324 THz, free-running quantum cascade laser with a THz Schottky-diode-balanced mixer using a swept, synthesized microwave source to drive the nonlinearity. Continuously tunable radiation of 1 μW power is demonstrated in two frequency regions: ν(Laser) ± 0 to 50 GHz and ν(Laser) ± 70 to 115 GHz. The sideband spectra were characterized with a Fourier-transform spectrometer, and the radiation was tuned through CO, HDO, and D2O rotational transitions.

Integrated Optical Interferometers with Micromachined Diaphragms for Pressure Sensing

NASA Technical Reports Server (NTRS)

DeBrabander, Gregory N.; Boyd, Joseph T.

1996-01-01

Optical pressure sensors have been fabricated which use an integrated optical channel waveguide that is part of an interferometer to measure the pressure-induced strain in a micromachined silicon diaphragm. A silicon substrate is etched from the back of the wafer leaving a rectangular diaphragm. On the opposite side of the wafer, ring resonator and Mach-Zehnder interferometers are formed with optical channel waveguides made from a low pressure chemical vapor deposited film of silicon oxynitride. The interferometer's phase is altered by pressure-induced stress in a channel segment positioned over the long edge of the diaphragm. The phase change in the ring resonator is monitored using a link-insensitive swept frequency laser diode, while in the Mach-Zehnder it is determined using a broad band super luminescent diode with subsequent wavelength separation. The ring resonator was found to be highly temperature sensitive, while the Mach-Zehnder, which had a smaller optical path length difference, was proportionally less so. The quasi-TM mode was more sensitive to pressure, in accord with calculations. Waveguide and sensor theory, sensitivity calculations, a fabrication sequence, and experimental results are presented.

Phase-locking and coherent power combining of broadband linearly chirped optical waves.

PubMed

Satyan, Naresh; Vasilyev, Arseny; Rakuljic, George; White, Jeffrey O; Yariv, Amnon

2012-11-05

We propose, analyze and demonstrate the optoelectronic phase-locking of optical waves whose frequencies are chirped continuously and rapidly with time. The optical waves are derived from a common optoelectronic swept-frequency laser based on a semiconductor laser in a negative feedback loop, with a precisely linear frequency chirp of 400 GHz in 2 ms. In contrast to monochromatic waves, a differential delay between two linearly chirped optical waves results in a mutual frequency difference, and an acoustooptic frequency shifter is therefore used to phase-lock the two waves. We demonstrate and characterize homodyne and heterodyne optical phase-locked loops with rapidly chirped waves, and show the ability to precisely control the phase of the chirped optical waveform using a digital electronic oscillator. A loop bandwidth of ~ 60 kHz, and a residual phase error variance of < 0.01 rad(2) between the chirped waves is obtained. Further, we demonstrate the simultaneous phase-locking of two optical paths to a common master waveform, and the ability to electronically control the resultant two-element optical phased array. The results of this work enable coherent power combining of high-power fiber amplifiers-where a rapidly chirping seed laser reduces stimulated Brillouin scattering-and electronic beam steering of chirped optical waves.

Tri-band optical coherence tomography for lipid and vessel spectroscopic imaging

NASA Astrophysics Data System (ADS)

Yu, Luoqin; Kang, Jiqiang; Wang, Xie; Wei, Xiaoming; Chan, Kin-Tak; Lee, Nikki P.; Wong, Kenneth K. Y.

2016-03-01

Optical coherence tomography (OCT) has been utilized for various functional imaging applications. One of its highlights comes from spectroscopic imaging, which can simultaneously obtain both morphologic and spectroscopic information. Assisting diagnosis and therapeutic intervention of coronary artery disease is one of the major directions in spectroscopic OCT applications. Previously Tanaka et al. have developed a spectral domain OCT (SDOCT) to image lipid distribution within blood vessel [1]. In the meantime, Fleming et al. have demonstrated optical frequency domain imaging (OFDI) by a 1.3-μm swept source and quadratic discriminant analysis model [2]. However, these systems suffered from burdensome computation as the optical properties' variation was calculated from a single-band illumination that provided limited contrast. On the other hand, multi-band OCT facilitates contrast enhancement with separated wavelength bands, which further offers an easier way to distinguish different materials. Federici and Dubois [3] and Tsai and Chan [4] have demonstrated tri-band OCT systems to further enhance the image contrast. However, these previous work provided under-explored functional properties. Our group has reported a dual-band OCT system based on parametrically amplified Fourier domain mode-locked (FDML) laser with time multiplexing scheme [5] and a dual-band FDML laser OCT system with wavelength-division multiplexing [6]. Fiber optical parametric amplifier (OPA) can be ideally incorporated in multi-band spectroscopic OCT system as it has a broad amplification window and offers an additional output range at idler band, which is phase matched with the signal band. The sweeping ranges can thus overcome traditional wavelength bands that are limited by intra-cavity amplifiers in FDML lasers. Here, we combines the dual-band FDML laser together with fiber OPA, which consequently renders a simultaneous tri-band output at 1.3, 1.5, and 1.6 μm, for intravascular applications. Lipid and blood vessel distribution can be subsequently visualized with the tri-band OCT system by ex vivo experiments using porcine artery model with artificial lipid plaques.

Development of Experimental Icing Simulation Capability for Full-Scale Swept Wings: Hybrid Design Process, Years 1 and 2

NASA Technical Reports Server (NTRS)

Fujiwara, Gustavo; Bragg, Mike; Triphahn, Chris; Wiberg, Brock; Woodard, Brian; Loth, Eric; Malone, Adam; Paul, Bernard; Pitera, David; Wilcox, Pete;

Laser cooling by adiabatic transfer

NASA Astrophysics Data System (ADS)

Norcia, Matthew; Cline, Julia; Bartolotta, John; Holland, Murray; Thompson, James

2017-04-01

We have demonstrated a new method of laser cooling applicable to particles with narrow linewidth optical transitions. This simple and robust cooling mechanism uses a frequency-swept laser to adiabatically transfer atoms between internal and motional states. The role of spontaneous emission is reduced (though is still critical) compared to Doppler cooling. This allows us to achieve greater slowing forces than would be possible with Doppler cooling, and may make this an appealing technique for cooling molecules. In this talk, I will present a demonstration of this technique in a cold strontium system. DARPA QUASAR, NIST, NSF PFC.

Optical air data systems and methods

NASA Technical Reports Server (NTRS)

Caldwell, Loren M. (Inventor); Tang, Shoou-Yu (Inventor); O'Brien, Martin J. (Inventor)

2009-01-01

A method for remotely sensing air outside a moving aircraft includes generating laser radiation within a swept frequency range. A portion of the laser radiation is projected from the aircraft into the air to induce scattered laser radiation. Filtered scattered laser radiation, filtered laser radiation, and unfiltered laser radiation are detected. At least one actual ratio is determined from data corresponding to the filtered scattered laser radiation and the unfiltered laser radiation. One or more air parameters are determined by correlating the actual ratio to at least one reference ratio.

Optical air data systems and methods

NASA Technical Reports Server (NTRS)

Caldwell, Loren M. (Inventor); O'Brien, Martin J. (Inventor); Tang, Shoou-Yu (Inventor)

2011-01-01

A method for remotely sensing air outside a moving aircraft includes generating laser radiation within a swept frequency range. A portion of the laser radiation is projected from the aircraft into the air to induce scattered laser radiation. Filtered scattered laser radiation, filtered laser radiation, and unfiltered laser radiation are detected. At least one actual ratio is determined from data corresponding to the filtered scattered laser radiation and the unfiltered laser radiation. One or more air parameters are determined by correlating the actual ratio to at least one reference ratio.

Experimental study of supersonic viscous leeside flow over a slender delta wing

NASA Technical Reports Server (NTRS)

Szodruch, J.

1980-01-01

An investigation was conducted to study in detail the vortical flow over the leeward side of a 70 deg swept delta wing having subsonic and supersonic leading edges. Two types of flow were encountered and studied, namely leading edge separation and separation with a shock. Especially for the latter type, Reynolds number plays an important role and unexpected strong streamwise vortices were observed. An optical method is described to obtain a first aproximation of shear stress values in the streamwise direction across the wing span.

Takeoff/approach noise for a model counterrotation propeller with a forward-swept upstream rotor

NASA Technical Reports Server (NTRS)

Woodward, Richard P.; Hall, David G.; Podboy, Gary G.; Jeracki, Robert J.

1993-01-01

A scale model of a counterrotating propeller with forward-swept blades in the forward rotor and aft-swept blades in the aft rotor (designated F39/A31) has been tested in the NASA Lewis 9- by 15-Foot Anechoic Wind Tunnel. This paper presents aeroacoustic results at a takeoff/approach condition of Mach 0.20. Laser Doppler velocimeter results taken in a plane between the two rotors are also included to quantify the interaction flow field. The intention of the forward-swept design is to reduce the magnitude of the forward rotor tip vortex and/or wakes which impinge on the aft rotor, thus lowering the interaction tone levels. A reference model propeller (designated F31/A31), having aft-swept blades in both rotors, was also tested. Aeroelastic performance of the F39/A31 propeller was disappointing. The forward rotor tip region tended to untwist toward higher effective blade angles under load. The forward rotor also exhibited steady state blade flutter at speeds and loadings well below the design condition. The noise results, based on sideline acoustic data, show that the interaction tone levels were up to 8 dB higher with the forward-swept design compared to those for the reference propeller at similar operating conditions, with these tone level differences extending down to lower propeller speeds where flutter did not occur. These acoustic results are for a poorly-performing forward-swept propeller. It is quite possible that a properly-designed forward-swept propeller would exhibit substantial interaction tone level reductions.

Frequency-agile gyrotron for electron decoupling and pulsed dynamic nuclear polarization

NASA Astrophysics Data System (ADS)

Scott, Faith J.; Saliba, Edward P.; Albert, Brice J.; Alaniva, Nicholas; Sesti, Erika L.; Gao, Chukun; Golota, Natalie C.; Choi, Eric J.; Jagtap, Anil P.; Wittmann, Johannes J.; Eckardt, Michael; Harneit, Wolfgang; Corzilius, Björn; Th. Sigurdsson, Snorri; Barnes, Alexander B.

2018-04-01

We describe a frequency-agile gyrotron which can generate frequency-chirped microwave pulses. An arbitrary waveform generator (AWG) within the NMR spectrometer controls the microwave frequency, enabling synchronized pulsed control of both electron and nuclear spins. We demonstrate that the acceleration of emitted electrons, and thus the microwave frequency, can be quickly changed by varying the anode voltage. This strategy results in much faster frequency response than can be achieved by changing the potential of the electron emitter, and does not require a custom triode electron gun. The gyrotron frequency can be swept with a rate of 20 MHz/μs over a 670 MHz bandwidth in a static magnetic field. We have already implemented time-domain electron decoupling with dynamic nuclear polarization (DNP) magic angle spinning (MAS) with this device. In this contribution, we show frequency-swept DNP enhancement profiles recorded without changing the NMR magnet or probe. The profile of endofullerenes exhibits a DNP profile with a <10 MHz linewidth, indicating that the device also has sufficient frequency stability, and therefore phase stability, to implement pulsed DNP mechanisms such as the frequency-swept solid effect. We describe schematics of the mechanical and vacuum construction of the device which includes a novel flanged sapphire window assembly. Finally, we discuss how commercially available continuous-wave gyrotrons can potentially be converted into similar frequency-agile high-power microwave sources.

Low-Reynolds Number Aerodynamics of an 8.9 Percent Scale Semispan Swept Wing for Assessment of Icing Effects

NASA Technical Reports Server (NTRS)

Broeren, Andy; Woodard, Brian; Diebold, Jeff; Moens, Frederic

2017-01-01

This paper presents the results of an experimental and computational study of low-Reynolds number swept wing aerodynamics. This work has been conducted in preparation for icing effects on a swept wing. A complete abstract will be written for the final paper.

Forward-swept wing configuration designed for high maneuverability by use of a transonic computational method

NASA Technical Reports Server (NTRS)

Mann, M. J.; Mercer, C. E.

1986-01-01

A transonic computational analysis method and a transonic design procedure have been used to design the wing and the canard of a forward-swept-wing fighter configuration for good transonic maneuver performance. A model of this configuration was tested in the Langley 16-Foot Transonic Tunnel. Oil-flow photographs were obtained to examine the wind flow patterns at Mach numbers from 0.60 to 0.90. The transonic theory gave a reasonably good estimate of the wing pressure distributions at transonic maneuver conditions. Comparison of the forward-swept-wing configuration with an equivalent aft-swept-wing-configuration showed that, at a Mach number of 0.90 and a lift coefficient of 0.9, the two configurations have the same trimmed drag. The forward-swept wing configuration was also found to have trimmed drag levels at transonic maneuver conditions which are comparable to those of the HiMAT (highly maneuverable aircraft technology) configuration and the X-29 forward-swept-wing research configuration. The configuration of this study was also tested with a forebody strake.

Analysis of swept-sine runs during modal identification

NASA Astrophysics Data System (ADS)

Gloth, G.; Sinapius, M.

2004-11-01

Experimental modal analysis of large aerospace structures in Europe combine nowadays the benefits of the very reliable but time-consuming phase resonance method and the application of phase separation techniques evaluating frequency response functions (FRF). FRFs of a test structure can be determined by a variety of means. Applied excitation signal waveforms include harmonic signals like stepped-sine excitation, periodic signals like multi-sine excitation, transient signals like impulse and swept-sine excitation, and stochastic signals like random. The current article focuses on slow swept-sine excitation which is a good trade-off between magnitude of excitation level needed for large aircraft and testing time. However, recent ground vibration tests (GVTs) brought up that reliable modal data from swept-sine test runs depend on a proper data processing. The article elucidates the strategy of modal analysis based on swept-sine excitation. The standards for the application of slowly swept sinusoids defined by the international organisation for standardisation in ISO 7626 part 2 are critically reviewed. The theoretical background of swept-sine testing is expounded with particular emphasis to the transition through structural resonances. The effect of different standard procedures of data processing like tracking filter, fast Fourier transform (FFT), and data reduction via averaging are investigated with respect to their influence on the FRFs and modal parameters. Particular emphasis is given to FRF distortions evoked by unsuitable data processing. All data processing methods are investigated on a numerical example. Their practical usefulness is demonstrated on test data taken from a recent GVT on a large aircraft. The revision of ISO 7626 part 2 is suggested regarding the application of slow swept-sine excitation. Recommendations about the proper FRF estimation from slow swept-sine excitation are given in order to enable the optimisation on these applications for future modal survey tests of large aerospace structures.

Extended-bandwidth frequency sweeps of a distributed feedback laser using combined injection current and temperature modulation

NASA Astrophysics Data System (ADS)

Hefferman, Gerald; Chen, Zhen; Wei, Tao

2017-07-01

This article details the generation of an extended-bandwidth frequency sweep using a single, communication grade distributed feedback (DFB) laser. The frequency sweep is generated using a two-step technique. In the first step, injection current modulation is employed as a means of varying the output frequency of a DFB laser over a bandwidth of 99.26 GHz. A digital optical phase lock loop is used to lock the frequency sweep speed during current modulation, resulting in a linear frequency chirp. In the second step, the temperature of the DFB laser is modulated, resulting in a shifted starting laser output frequency. A laser frequency chirp is again generated beginning at this shifted starting frequency, resulting in a frequency-shifted spectrum relative to the first recorded data. This process is then repeated across a range of starting temperatures, resulting in a series of partially overlapping, frequency-shifted spectra. These spectra are then aligned using cross-correlation and combined using averaging to form a single, broadband spectrum with a total bandwidth of 510.9 GHz. In order to investigate the utility of this technique, experimental testing was performed in which the approach was used as the swept-frequency source of a coherent optical frequency domain reflectometry system. This system was used to interrogate an optical fiber containing a 20 point, 1-mm pitch length fiber Bragg grating, corresponding to a period of 100 GHz. Using this technique, both the periodicity of the grating in the frequency domain and the individual reflector elements of the structure in the time domain were resolved, demonstrating the technique's potential as a method of extending the sweeping bandwidth of semiconductor lasers for frequency-based sensing applications.

Imaging in vivo secondary caries and ex vivo dental biofilms using cross-polarization optical coherence tomography

PubMed Central

Lenton, Pat; Rudney, Joel; Chen, Ruoqiong; Fok, Alex; Aparicio, Conrado; Jones, Robert S.

2012-01-01

Objectives Conventional diagnostic methods frequently detect only late stage enamel demineralization under composite resin restorations. The objective of this study is to examine the subsurface tooth-composite interface and to assess for the presence of secondary caries in pediatric patients using a novel Optical Coherence Tomography System with an intraoral probe. Methods A newly designed intraoral cross polarization swept source optical coherence tomography (CP-OCT) imaging system was used to examine the integrity of the enamel-composite interfaces in vivo. Twenty two pediatric subjects were recruited with either recently placed or long standing composite restorations in their primary teeth. To better understand how bacterial biofilms cause demineralization at the interface, we also used the intraoral CP-OCT system to assess ex vivo bacterial biofilm growth on dental composites. Results As a positive control, cavitated secondary carious interfaces showed a 18.2 dB increase (p<0.001), or over 1-2 orders of magnitude higher, scattering than interfaces associated with recently placed composite restorations. Several long standing composite restorations, which appeared clinically sound, had a marked increase in scattering than recently placed restorations. This suggests the ability of CP-OCT to assess interfacial degradation such as early secondary caries prior to cavitation. CP-OCT was also able to image ex vivo biofilms on dental composites and assess their thickness. Significance This paper shows that CP-OCT imaging using a beam splitter based design can examine the subsurface interface of dental composites in human subjects. Furthermore, the probe dimensions and acquisition speed of the CP-OCT system allowed for analysis of caries development in children. PMID:22578989

Full-range k-domain linearization in spectral-domain optical coherence tomography.

PubMed

Jeon, Mansik; Kim, Jeehyun; Jung, Unsang; Lee, Changho; Jung, Woonggyu; Boppart, Stephen A

2011-03-10

A full-bandwidth k-domain linearization method for spectral-domain optical coherence tomography (SD-OCT) is demonstrated. The method uses information of the wavenumber-pixel-position provided by a translating-slit-based wavelength filter. For calibration purposes, the filter is placed either after a broadband source or at the end of the sample path, and the filtered spectrum with a narrowed line width (∼0.5 nm) is incident on a line-scan camera in the detection path. The wavelength-swept spectra are co-registered with the pixel positions according to their central wavelengths, which can be automatically measured with an optical spectrum analyzer. For imaging, the method does not require a filter or a software recalibration algorithm; it simply resamples the OCT signal from the detector array without employing rescaling or interpolation methods. The accuracy of k-linearization is maximized by increasing the k-linearization order, which is known to be a crucial parameter for maintaining a narrow point-spread function (PSF) width at increasing depths. The broadening effect is studied by changing the k-linearization order by undersampling to search for the optimal value. The system provides more position information, surpassing the optimum without compromising the imaging speed. The proposed full-range k-domain linearization method can be applied to SD-OCT systems to simplify their hardware/software, increase their speed, and improve the axial image resolution. The experimentally measured width of PSF in air has an FWHM of 8 μm at the edge of the axial measurement range. At an imaging depth of 2.5 mm, the sensitivity of the full-range calibration case drops less than 10 dB compared with the uncompensated case.

Multimodal ophthalmic imaging using spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography

NASA Astrophysics Data System (ADS)

El-Haddad, Mohamed T.; Malone, Joseph D.; Li, Jianwei D.; Bozic, Ivan; Arquitola, Amber M.; Joos, Karen M.; Patel, Shriji N.; Tao, Yuankai K.

2017-08-01

Ophthalmic surgery involves manipulation of delicate, layered tissue structures on milli- to micrometer scales. Traditional surgical microscopes provide an inherently two-dimensional view of the surgical field with limited depth perception which precludes accurate depth-resolved visualization of these tissue layers, and limits the development of novel surgical techniques. We demonstrate multimodal swept-source spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography (SS-SESLO-OCT) to address current limitations of image-guided ophthalmic microsurgery. SS-SESLO-OCT provides inherently co-registered en face and cross-sectional field-of-views (FOVs) at a line rate of 400 kHz and >2 GPix/s throughput. We show in vivo imaging of the anterior segment and retinal fundus of a healthy volunteer, and preliminary results of multi-volumetric mosaicking for ultrawide-field retinal imaging with 90° FOV. Additionally, a scan-head was rapid-prototyped with a modular architecture which enabled integration of SS-SESLO-OCT with traditional surgical microscope and slit-lamp imaging optics. Ex vivo surgical maneuvers were simulated in cadaveric porcine eyes. The system throughput enabled volumetric acquisition at 10 volumes-per-second (vps) and allowed visualization of surgical dynamics in corneal sweeps, compressions, and dissections, and retinal sweeps, compressions, and elevations. SESLO en face images enabled simple real-time co-registration with the surgical microscope FOV, and OCT cross-sections provided depth-resolved visualization of instrument-tissue interactions. Finally, we demonstrate novel augmented-reality integration with the surgical view using segmentation overlays to aid surgical guidance. SS-SESLO-OCT may benefit clinical diagnostics by enabling aiming, registration, and mosaicking; and intraoperative imaging by allowing for real-time surgical feedback, instrument tracking, and overlays of computationally extracted biomarkers of disease.

Truly simultaneous SS-OCT of the anterior and posterior human eye with full anterior chamber and 50° retinal field of views (Conference Presentation)

NASA Astrophysics Data System (ADS)

McNabb, Ryan P.; Viehland, Christian; Keller, Brenton; Vann, Robin R.; Izatt, Joseph A.; Kuo, Anthony N.

2017-02-01

Optical coherence tomography (OCT) has revolutionized clinical observation of the eye and is an indispensable part of the modern ophthalmic practice. Unlike many other ophthalmic imaging techniques, OCT provides three-dimensional information about the imaged eye. However, conventional clinical OCT systems image only the anterior or the posterior eye during a single acquisition. Newer OCT systems have begun to image both during the same acquisition but with compromises such as limited field of view in the posterior eye or requiring rapid switching between the anterior and posterior eye during the scan. We describe here the development and demonstration of an OCT system with truly simultaneous imaging of both the anterior and posterior eye capable of imaging the full anterior chamber width and 50° on the retina (macula, optic nerve, and arcades). The whole eye OCT system was developed using custom optics and optomechanics. Polarization was utilized to separate the imaging channels. We utilized a 200kHz swept-source laser (Axsun Technologies) centered at 1040±50nm of bandwidth. The clock signal generated by the laser was interpolated 4x to generate 5504 samples per laser sweep. With the whole eye OCT system, we simultaneously acquired anterior and posterior segments with repeated B-scans as well as three-dimensional volumes from seven healthy volunteers (other than refractive error). On three of these volunteers, whole eye OCT and partial coherence interferometry (LenStar PCI, Haag-Streit) were used to measure axial eye length. We measured a mean repeatability of ±47µm with whole eye OCT and a mean difference from PCI of -68µm.

Extended-bandwidth frequency sweeps of a distributed feedback laser using combined injection current and temperature modulation.

PubMed

Hefferman, Gerald; Chen, Zhen; Wei, Tao

2017-07-01

This article details the generation of an extended-bandwidth frequency sweep using a single, communication grade distributed feedback (DFB) laser. The frequency sweep is generated using a two-step technique. In the first step, injection current modulation is employed as a means of varying the output frequency of a DFB laser over a bandwidth of 99.26 GHz. A digital optical phase lock loop is used to lock the frequency sweep speed during current modulation, resulting in a linear frequency chirp. In the second step, the temperature of the DFB laser is modulated, resulting in a shifted starting laser output frequency. A laser frequency chirp is again generated beginning at this shifted starting frequency, resulting in a frequency-shifted spectrum relative to the first recorded data. This process is then repeated across a range of starting temperatures, resulting in a series of partially overlapping, frequency-shifted spectra. These spectra are then aligned using cross-correlation and combined using averaging to form a single, broadband spectrum with a total bandwidth of 510.9 GHz. In order to investigate the utility of this technique, experimental testing was performed in which the approach was used as the swept-frequency source of a coherent optical frequency domain reflectometry system. This system was used to interrogate an optical fiber containing a 20 point, 1-mm pitch length fiber Bragg grating, corresponding to a period of 100 GHz. Using this technique, both the periodicity of the grating in the frequency domain and the individual reflector elements of the structure in the time domain were resolved, demonstrating the technique's potential as a method of extending the sweeping bandwidth of semiconductor lasers for frequency-based sensing applications.

High resolution multimodal clinical ophthalmic imaging system

PubMed Central

Mujat, Mircea; Ferguson, R. Daniel; Patel, Ankit H.; Iftimia, Nicusor; Lue, Niyom; Hammer, Daniel X.

2010-01-01

We developed a multimodal adaptive optics (AO) retinal imager which is the first to combine high performance AO-corrected scanning laser ophthalmoscopy (SLO) and swept source Fourier domain optical coherence tomography (SSOCT) imaging modes in a single compact clinical prototype platform. Such systems are becoming ever more essential to vision research and are expected to prove their clinical value for diagnosis of retinal diseases, including glaucoma, diabetic retinopathy (DR), age-related macular degeneration (AMD), and retinitis pigmentosa. The SSOCT channel operates at a wavelength of 1 µm for increased penetration and visualization of the choriocapillaris and choroid, sites of major disease activity for DR and wet AMD. This AO system is designed for use in clinical populations; a dual deformable mirror (DM) configuration allows simultaneous low- and high-order aberration correction over a large range of refractions and ocular media quality. The system also includes a wide field (33 deg.) line scanning ophthalmoscope (LSO) for initial screening, target identification, and global orientation, an integrated retinal tracker (RT) to stabilize the SLO, OCT, and LSO imaging fields in the presence of lateral eye motion, and a high-resolution LCD-based fixation target for presentation of visual cues. The system was tested in human subjects without retinal disease for performance optimization and validation. We were able to resolve and quantify cone photoreceptors across the macula to within ~0.5 deg (~100-150 µm) of the fovea, image and delineate ten retinal layers, and penetrate to resolve features deep into the choroid. The prototype presented here is the first of a new class of powerful flexible imaging platforms that will provide clinicians and researchers with high-resolution, high performance adaptive optics imaging to help guide therapies, develop new drugs, and improve patient outcomes. PMID:20589021

Approximate relations and charts for low-speed stability derivatives of swept wings

NASA Technical Reports Server (NTRS)

Toll, Thomas A; Queijo, M J

1948-01-01

Contains derivations, based on a simplified theory, of approximate relations for low-speed stability derivatives of swept wings. Method accounts for the effects and, in most cases, taper ratio. Charts, based on the derived relations, are presented for the stability derivatives of untapered swept wings. Calculated values of the derivatives are compared with experimental results.

Normal injection of helium from swept struts into ducted supersonic flow

NASA Technical Reports Server (NTRS)

Mcclinton, C. R.; Torrence, M. G.

1975-01-01

Recent design studies have shown that airframe-integrated scramjets should include instream mounted, swept-back strut fuel injectors to obtain short combustors. Because there was no data in the literature on mixing characteristics of swept strut fuel injectors, the present investigation was undertaken to provide such data. This investigation was made with two swept struts in a closed duct at Mach number of 4.4 and nominal jet-to-air mass flow ratio of 0.029 with helium used to simulate hydrogen fuel. The data is compared with flat plate mounted normal injector data to obtain the effect of swept struts on mixing. Three injector patterns were evaluated representing the range of hole spacing and jet-to-freestream dynamic pressure ratio of interest. Measured helium concentration, pitot pressure, and static pressure in the downstream mixing region are used to generate contour plots necessary to define the mixing region flow field and the mixing parameters.

Vertical comb-drive microscanner with 4x4 array of micromirrors for phase-shifting Mirau microinterferometry

NASA Astrophysics Data System (ADS)

Bargiel, Sylwester; Lullin, Justine; Lemoal, Patrice; Perrin, Stéphane; Passilly, Nicolas; Albero, Jorge; Froehly, Luc; Lardet-Vieudrin, Franck; Gorecki, Christophe

2016-04-01

In this paper, we present construction, fabrication and characterization of an electrostatic MOEMS vertical microscanner for generation of an optical phase shift in array-type interferometric microsystems. The microscanner employs asymmetric comb-drives for a vertical displacement of a large 4x4 array of reference micromirrors and for in-situ position sensing. The device is designed to be fully compatible with Mirau configuration and with vertical integration strategy. This enables further integration of the device within an "active" multi-channel Mirau micro-interferometer and implementation of the phase shifting interferometry (PSI) technique for imaging applications. The combination of micro-interferometer and PSI is particularly interesting in the swept-source optical coherence tomography, since it allows not only strong size reduction of a system but also improvement of its performance (sensitivity, removal of the image artefacts). The technology of device is based on double-side DRIE of SOI wafer and vapor HF releasing of the suspended platform. In the static mode, the device provides vertical displacement of micromirrors up to 2.8μm (0 - 40V), whereas at resonance (fo=500 Hz), it reaches 0.7 μm for only 1VDC+1VAC. In both operation modes, the measured displacement is much more than required for PSI implementation (352nm peak-to-peak). The presented device is a key component of array-type Mirau micro-interferometer that enables the construction of portable, low-cost interferometric systems, e.g. for in vivo medical diagnostics.

Variations in optical coherence tomography resolution and uniformity: a multi-system performance comparison

PubMed Central

Fouad, Anthony; Pfefer, T. Joshua; Chen, Chao-Wei; Gong, Wei; Agrawal, Anant; Tomlins, Peter H.; Woolliams, Peter D.; Drezek, Rebekah A.; Chen, Yu

2014-01-01

Point spread function (PSF) phantoms based on unstructured distributions of sub-resolution particles in a transparent matrix have been demonstrated as a useful tool for evaluating resolution and its spatial variation across image volumes in optical coherence tomography (OCT) systems. Measurements based on PSF phantoms have the potential to become a standard test method for consistent, objective and quantitative inter-comparison of OCT system performance. Towards this end, we have evaluated three PSF phantoms and investigated their ability to compare the performance of four OCT systems. The phantoms are based on 260-nm-diameter gold nanoshells, 400-nm-diameter iron oxide particles and 1.5-micron-diameter silica particles. The OCT systems included spectral-domain and swept source systems in free-beam geometries as well as a time-domain system in both free-beam and fiberoptic probe geometries. Results indicated that iron oxide particles and gold nanoshells were most effective for measuring spatial variations in the magnitude and shape of PSFs across the image volume. The intensity of individual particles was also used to evaluate spatial variations in signal intensity uniformity. Significant system-to-system differences in resolution and signal intensity and their spatial variation were readily quantified. The phantoms proved useful for identification and characterization of irregularities such as astigmatism. Our multi-system results provide evidence of the practical utility of PSF-phantom-based test methods for quantitative inter-comparison of OCT system resolution and signal uniformity. PMID:25071949

Wavelength tunable MEMS VCSELs for OCT imaging

NASA Astrophysics Data System (ADS)

Sahoo, Hitesh Kumar; Ansbæk, Thor; Ottaviano, Luisa; Semenova, Elizaveta; Hansen, Ole; Yvind, Kresten

2018-02-01

MEMS VCSELs are one of the most promising swept source (SS) lasers for optical coherence tomography (OCT) and one of the best candidates for future integration with endoscopes, surgical probes and achieving an integrated OCT system. However, the current MEMS-based SS are processed on the III-V wafers, which are small, expensive and challenging to work with. Furthermore, the actuating part, i.e., the MEMS, is on the top of the structure which causes a strong dependence on packaging to decrease its sensitivity to the operating environment. This work addresses these design drawbacks and proposes a novel design framework. The proposed device uses a high contrast grating mirror on a Si MEMS stage as the bottom mirror, all of which is defined in an SOI wafer. The SOI wafer is then bonded to an InP III-V wafer with the desired active layers, thereby sealing the MEMS. Finally, the top mirror, a dielectric DBR (7 pairs of TiO2 - SiO2), is deposited on top. The new device is based on a silicon substrate with MEMS defined on a silicon membrane in an enclosed cavity. Thus the device is much more robust than the existing MEMS VCSELs. This design also enables either a two-way actuation on the MEMS or a smaller optical cavity (pull-away design), i.e., wider FSR (Free Spectral Range) to increase the wavelength sweep. Fabrication of the proposed device is outlined and the results of device characterization are reported.

Development of a high-speed VCSEL OCT system for real-time imaging of conscious patients larynx using a hand-held probe (Conference Presentation)

NASA Astrophysics Data System (ADS)

Rangarajan, Swathi; Chou, Li-Dek; Coughlan, Carolyn; Sharma, Giriraj; Wong, Brian J. F.; Ramalingam, Tirunelveli S.

2016-02-01

Fourier domain optical coherence tomography (FD-OCT) is a noninvasive imaging modality that has previously been used to image the human larynx. However, differences in anatomical geometry and short imaging range of conventional OCT limits its application in a clinical setting. In order to address this issue, we have developed a gradient-index (GRIN) lens rod-based hand-held probe in conjunction with a long imaging range 200 kHz Vertical-Cavity Surface Emitting Lasers (VCSEL) swept-source optical coherence tomography (SS-OCT) system for high speed real-time imaging of the human larynx in an office setting. This hand-held probe is designed to have a long and dynamically tunable working distance to accommodate the differences in anatomical geometry of human test subjects. A nominal working distance (~6 cm) of the probe is selected to have a lateral resolution <100 um within a depth of focus of 6.4 mm, which covers more than half of the 12 mm imaging range of the VCSEL laser. The maximum lateral scanning range of the probe at 6 cm working distance is approximately 8.4 mm, and imaging an area of 8.5 mm by 8.5 mm is accomplished within a second. Using the above system, we will demonstrate real-time cross-sectional OCT imaging of larynx during phonation in vivo in human and ex-vivo in pig vocal folds.

Airborne Laser CO2 Column Measurements: Evaluation of Precision and Accuracy Under a Wide Range of Surface and Atmospheric Conditions

NASA Astrophysics Data System (ADS)

Browell, E. V.; Dobler, J. T.; Kooi, S. A.; Fenn, M. A.; Choi, Y.; Vay, S. A.; Harrison, F. W.; Moore, B.

2011-12-01

This paper discusses the latest flight test results of a multi-frequency intensity-modulated (IM) continuous-wave (CW) laser absorption spectrometer (LAS) that operates near 1.57 μm for remote CO2 column measurements. This IM-LAS system is under development for a future space-based mission to determine the global distribution of regional-scale CO2 sources and sinks, which is the objective of the NASA Active Sensing of CO2 Emissions during Nights, Days, and Seasons (ASCENDS) mission. A prototype of the ASCENDS system, called the Multi-frequency Fiber Laser Lidar (MFLL), has been flight tested in eleven airborne campaigns since May 2005. This paper compares the most recent results obtained during the 2010 and 2011 UC-12 and DC-8 flight tests, where MFLL remote CO2 column measurements were evaluated against airborne in situ CO2 profile measurements traceable to World Meteorological Organization standards. The major change to the MFLL system in 2011 was the implementation of several different IM modes, which could be quickly changed in flight, to directly compare the precision and accuracy of MFLL CO2 measurements in each mode. The different IM modes that were evaluated included "fixed" IM frequencies near 50, 200, and 500 kHz; frequencies changed in short time steps (Stepped); continuously swept frequencies (Swept); and a pseudo noise (PN) code. The Stepped, Swept, and PN modes were generated to evaluate the ability of these IM modes to desensitize MFLL CO2 column measurements to intervening optically thin aerosols/clouds. MFLL was flown on the NASA Langley UC-12 aircraft in May 2011 to evaluate the newly implemented IM modes and their impact on CO2 measurement precision and accuracy, and to determine which IM mode provided the greatest thin cloud rejection (TCR) for the CO2 column measurements. Within the current hardware limitations of the MFLL system, the "fixed" 50 kHz results produced similar SNR values to those found previously. The SNR decreased as expected with increasing IM frequency with the SNR(500 kHz) equal to 31% of SNR(50 kHz). The absolute accuracy of the 50 kHz CO2 measurement showed a previously observed altitude-dependent trend that was greatly reduced at 200 kHz. Laboratory experiments have duplicated this effect which results mainly from IM frequency cross talk between LAS wavelengths in the erbium-doped fiber amplifier (EDFA) and which is reduced when operating at higher IM frequencies. Performance of the Stepped, Swept, and PN modes were evaluated in close time proximity to each other, and these results will be discussed in this paper. A second series of ASCENDS flight tests were conducted on the NASA DC-8 from 25 July to 12 August 2011 over similar local land and ocean targets as in 2010 and with additional long-range flights planned over the corn fields of Iowa, forests in northern Wisconsin, and ice fields of southeastern Alaska. MFLL CO2 measurement results from this field campaign will also be presented.

Transparent Conveyor of Dielectric Liquids or Particles

NASA Technical Reports Server (NTRS)

Calle, Carlos I.; Mantovani, James G.

2009-01-01

The concept of a transparent conveyor of small loose dielectric parti cles or small amounts of dielectric liquids has emerged as an outgro wth of an effort to develop efficient, reliable means of automated re moval of dust from solar cells and from windows of optical instrumen ts. This concept is based on the previously reported concept of an e lectrodynamic screen, according to which a grid-like electric field is established on and near a surface and is moved along the surface p erpendicularly to the grid lines. The resulting electrodynamic force s on loose dielectric particles or dielectric liquid drops in the vic inity would move the particles or drops along the surface. In the or iginal dust-removal application, dust particles would thus be swept out of the affected window area. Other potential applications may occ ur in nanotechnology -- for example, involving mixing of two or more fluids and/or nanoscale particles under optical illumination and/or optical observation.

Digitally synthesized beat frequency-multiplexed fluorescence lifetime spectroscopy

PubMed Central

Chan, Jacky C. K.; Diebold, Eric D.; Buckley, Brandon W.; Mao, Sien; Akbari, Najva; Jalali, Bahram

2014-01-01

Frequency domain fluorescence lifetime imaging is a powerful technique that enables the observation of subtle changes in the molecular environment of a fluorescent probe. This technique works by measuring the phase delay between the optical emission and excitation of fluorophores as a function of modulation frequency. However, high-resolution measurements are time consuming, as the excitation modulation frequency must be swept, and faster low-resolution measurements at a single frequency are prone to large errors. Here, we present a low cost optical system for applications in real-time confocal lifetime imaging, which measures the phase vs. frequency spectrum without sweeping. Deemed Lifetime Imaging using Frequency-multiplexed Excitation (LIFE), this technique uses a digitally-synthesized radio frequency comb to drive an acousto-optic deflector, operated in a cat’s-eye configuration, to produce a single laser excitation beam modulated at multiple beat frequencies. We demonstrate simultaneous fluorescence lifetime measurements at 10 frequencies over a bandwidth of 48 MHz, enabling high speed frequency domain lifetime analysis of single- and multi-component sample mixtures. PMID:25574449

On-line structural health and fire monitoring of a composite personal aircraft using an FBG sensing system

NASA Astrophysics Data System (ADS)

Chandler, K.; Ferguson, S.; Graver, T.; Csipkes, A.; Mendez, A.

2008-03-01

We report in this paper on the design and development of a novel on-line structural health monitoring and fire detection system based on an array of optical fiber Bragg grating (FBG) sensors and interrogation system installed on a new, precommercial compact aircraft. A combined total of 17 FBG sensors - strain, temperature and high-temperature - were installed at critical locations in an around the wings, fuselage and engine compartment of a prototype, Comp Air CA 12 all-composite, ten-passenger personal airplane powered by a 1,650 hp turbine engine. The sensors are interrogated online and in real time by a swept laser FBG interrogator (Micron Optics sm125-700) mounted on board the plane. Sensors readings are then combined with the plane's avionics system and displayed on the pilot's aviation control panel. This system represents the first of its kind in commercial, small frame, airplanes and a first for optical fiber sensors.

Dual wing, swept forward swept rearward wing, and single wing design optimization for high performance business airplanes

NASA Technical Reports Server (NTRS)

Rhodes, M. D.; Selberg, B. P.

1982-01-01

An investigation was performed to compare closely coupled dual wing and swept forward swept rearward wing aircraft to corresponding single wing 'baseline' designs to judge the advantages offered by aircraft designed with multiple wing systems. The optimum multiple wing geometry used on the multiple wing designs was determined in an analytic study which investigated the two- and three-dimensional aerodynamic behavior of a wide range of multiple wing configurations in order to find the wing geometry that created the minimum cruise drag. This analysis used a multi-element inviscid vortex panel program coupled to a momentum integral boundary layer analysis program to account for the aerodynamic coupling between the wings and to provide the two-dimensional aerodynamic data, which was then used as input for a three-dimensional vortex lattice program, which calculated the three-dimensional aerodynamic data. The low drag of the multiple wing configurations is due to a combination of two dimensional drag reductions, tailoring the three dimensional drag for the swept forward swept rearward design, and the structural advantages of the two wings that because of the structural connections permitted higher aspect ratios.

Optical magnetic resonances induced by the interference of reactive components in the near radiation-field zone of atoms in a glow discharge of a mixture of even neon isotopes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Saprykin, E. G., E-mail: Saprykin@gorodok.net

2016-02-15

Four types of anomalous optical magnetic resonances shifted with respect to the zero magnetic field and with different shapes are found in radiation of a glow discharge in a mixture of even neon isotopes placed in a swept longitudinal magnetic field. This testifies to the manifestation of collective processes of synchronous light emission by oscillators belonging to isotopically different spatially separated atoms in discharge plasma. The origin of resonances is associated with nonstationary interference of reactive fields in the near radiation-field zones of emission of atoms, averaged over the lifetime of the fields (interference), while different types of resonances aremore » associated with different methods of synchronization of the phases of the fields.« less

Swept-Wing Ice Accretion Characterization and Aerodynamics

NASA Technical Reports Server (NTRS)

Broeren, Andy P.; Potapczuk, Mark G.; Riley, James T.; Villedieu, Philippe; Moens, Frederic; Bragg, Michael B.

2013-01-01

NASA, FAA, ONERA, the University of Illinois and Boeing have embarked on a significant, collaborative research effort to address the technical challenges associated with icing on large-scale, three-dimensional swept wings. The overall goal is to improve the fidelity of experimental and computational simulation methods for swept-wing ice accretion formation and resulting aerodynamic effect. A seven-phase research effort has been designed that incorporates ice-accretion and aerodynamic experiments and computational simulations. As the baseline, full-scale, swept-wing-reference geometry, this research will utilize the 65% scale Common Research Model configuration. Ice-accretion testing will be conducted in the NASA Icing Research Tunnel for three hybrid swept-wing models representing the 20%, 64% and 83% semispan stations of the baseline-reference wing. Three-dimensional measurement techniques are being developed and validated to document the experimental ice-accretion geometries. Artificial ice shapes of varying geometric fidelity will be developed for aerodynamic testing over a large Reynolds number range in the ONERA F1 pressurized wind tunnel and in a smaller-scale atmospheric wind tunnel. Concurrent research will be conducted to explore and further develop the use of computational simulation tools for ice accretion and aerodynamics on swept wings. The combined results of this research effort will result in an improved understanding of the ice formation and aerodynamic effects on swept wings. The purpose of this paper is to describe this research effort in more detail and report on the current results and status to date. 1

Swept-Wing Ice Accretion Characterization and Aerodynamics

NASA Technical Reports Server (NTRS)

Broeren, Andy P.; Potapczuk, Mark G.; Riley, James T.; Villedieu, Philippe; Moens, Frederic; Bragg, Michael B.

2013-01-01

NASA, FAA, ONERA, the University of Illinois and Boeing have embarked on a significant, collaborative research effort to address the technical challenges associated with icing on large-scale, three-dimensional swept wings. The overall goal is to improve the fidelity of experimental and computational simulation methods for swept-wing ice accretion formation and resulting aerodynamic effect. A seven-phase research effort has been designed that incorporates ice-accretion and aerodynamic experiments and computational simulations. As the baseline, full-scale, swept-wing-reference geometry, this research will utilize the 65 percent scale Common Research Model configuration. Ice-accretion testing will be conducted in the NASA Icing Research Tunnel for three hybrid swept-wing models representing the 20, 64 and 83 percent semispan stations of the baseline-reference wing. Threedimensional measurement techniques are being developed and validated to document the experimental ice-accretion geometries. Artificial ice shapes of varying geometric fidelity will be developed for aerodynamic testing over a large Reynolds number range in the ONERA F1 pressurized wind tunnel and in a smaller-scale atmospheric wind tunnel. Concurrent research will be conducted to explore and further develop the use of computational simulation tools for ice accretion and aerodynamics on swept wings. The combined results of this research effort will result in an improved understanding of the ice formation and aerodynamic effects on swept wings. The purpose of this paper is to describe this research effort in more detail and report on the current results and status to date.

Effects of a Forward-swept Front Rotor on the Flowfield of a Counterrotation Propeller

NASA Technical Reports Server (NTRS)

Nallasamy, M.; Podboy, Gary G.

1994-01-01

The effects of a forward-swept front rotor on the flowfield of a counterrotation model propeller at takeoff conditions at zero degree angle of attack are studied by solving the unsteady three-dimensional Euler equations. The configuration considered is an uneven blade count counterrotation model with twelve forward-swept blades on the fore rotor and ten aft-swept blades on the aft rotor. The flowfield is compared with that of a reference aft-swept counterrotation geometry and Laser Doppler Velocimeter (LDV) measurements. At the operating conditions considered, the forward-swept blade experiences a higher tip loading and produces a stronger tip vortex compared to the aft-swept blade, consistent with the LDV and acoustic measurements. Neither the solution nor the LDV data indicated the formation of a leading edge vortex. The predicted radial distribution of the circumferentially averaged axial velocity at the measurement station agreed very closely with LDV data, while crossflow velocities showed poor agreement. The discrepancy between prediction and LDV data of tangential and radial velocities is due in part to the insufficient mesh resolution in the region between the rotors and in the tip region to track the tip vortex. The vortex is diffused by the time it arrives at the measurement station. The uneven blade count configuration requires the solution to be carried out for six blade passages of the fore rotor and five passages of the aft rotor, thus making grid refinement prohibitive.

Probing the pre-PN Mass Loss Histories in the PPN Dust Shells

NASA Astrophysics Data System (ADS)

Ueta, T.

2001-12-01

Proto-planetary nebulae (PPNs) are immediate progenitors of planetary nebulae (PNs) rapidly evolving over a relatively short time scale. Unlike the full-fledged PNs, the circumstellar dust shells of PPNs have neither been photo-ionized nor been swept up by fast winds. Since the PPN shells retain pristine fossil records of mass loss histories of these stars during the pre-PN phases, these dust shells provide ideal astronomical laboratories in which to investigate the origin of complex PN structures that we observe. We have conducted imaging surveys of the PPN shells in mid-infrared and optical wavelengths, probing the dust distribution directly via mid-infrared thermal dust emission arising from the shells and indirectly via dust-scattered stellar optical emission passing through the shells. From these surveys, we have found that (1) the PPN shells are intrinsically axisymmetric due to equatorially-enhanced superwind mass loss that occurred immediately before the beginning of the PPN phase, and (2) the variable degree of equatorial enhancement in the shells, which is probably related to the progenitor mass, has resulted in different optical depths and morphologies. To characterize the PPN shell geometries, we have developed and employed a 2.5 dimensional radiative transfer code that treats dust absorption, reemission, and an/isotropic scattering in any axisymmetric system illuminated by a central energy source. In the code, the dust optical properties are derived from the laboratory-measured refractive index using Mie theory allowing a distribution of sizes for each species in each composition layer in the shell. Our numerical analysis would be able to de-project and recover 3-D geometrical quantities, such as the pole-to-equator density ratio, from the observational data. These model calculations would provide constraining parameters for hydrodynamical models intended to generate equatorial enhancements during dust mass loss as well as initial parameters for magneto-hydrodynamical models aimed to reproduce highly complex PN morphologies.

Apparatus and method for non-contact, acoustic resonance determination of intraocular pressure

DOEpatents

Sinha, Dipen N.; Wray, William O.

1994-01-01

Apparatus and method for measuring intraocular pressure changes in an eye under investigation by detection of vibrational resonances therein. An ultrasonic transducer operating at its resonant frequency is amplitude modulated and swept over a range of audio frequencies in which human eyes will resonate. The output therefrom is focused onto the eye under investigation, and the resonant vibrations of the eye observed using a fiber-optic reflection vibration sensor. Since the resonant frequency of the eye is dependent on the pressure therein, changes in intraocular pressure may readily be determined after a baseline pressure is established.

Apparatus and method for non-contact, acoustic resonance determination of intraocular pressure

DOEpatents

Sinha, D.N.; Wray, W.O.

1994-12-27

The apparatus and method for measuring intraocular pressure changes in an eye under investigation by detection of vibrational resonances therein. An ultrasonic transducer operating at its resonant frequency is amplitude modulated and swept over a range of audio frequencies in which human eyes will resonate. The output therefrom is focused onto the eye under investigation, and the resonant vibrations of the eye observed using a fiber-optic reflection vibration sensor. Since the resonant frequency of the eye is dependent on the pressure therein, changes in intraocular pressure may readily be determined after a baseline pressure is established. 3 figures.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zohar, S.; Sterbinsky, G. E.

Here, we propose an experimental technique for extending feedback compensation of dissipative radiation used in nuclear magnetic resonance (NMR) to encompass ferromagnetic resonance (FMR). This method uses a balanced microwave power detector whose output is phase shifted π/2, amplified, and fed back to drive precession. Using classical control theory, we predict an electronically controllable narrowing of field swept FMR line-widths. This technique is predicted to compensate other sources of spin dissipation in addition to radiative loss.

Demonstration of a rapidly-swept external cavity quantum cascade laser for atmospheric sensing applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brumfield, Brian E.; Taubman, Matthew S.; Phillips, Mark C.

2016-07-01

The application of quantum cascade lasers (QCLs) in atmospheric science for trace detection of gases has been demonstrated using sensors in point or remote sensing configurations. Many of these systems utilize single narrowly-tunable (~10 cm-1) distributed feedback (DFB-) QCLs that limit simultaneous detection to a restricted number of small chemical species like H2O or N2O. The narrow wavelength range of DFB-QCLs precludes accurate quantification of large chemical species with broad rotationally-unresolved vibrational spectra, such as volatile organic compounds, that play an important role in the chemistry of the atmosphere. External-cavity (EC-) QCL systems are available that offer tuning ranges >100more » cm-1, making them excellent IR sources for measuring multiple small and large chemical species in the atmosphere. While the broad wavelength coverage afforded by an EC system enables measurements of large chemical species, most commercial systems can only be swept over their entire wavelength range at less than 10 Hz. This prohibits broadband simultaneous measurements of multiple chemicals in plumes from natural or industrial sources where turbulence and/or chemical reactivity are resulting in rapid changes in chemical composition on sub-1s timescales. At Pacific Northwest National Laboratory we have developed rapidly-swept EC-QCL technology that acquires broadband absorption spectra (~100 cm-1) on ms timescales. The spectral resolution of this system has enabled simultaneous measurement of narrow rotationally-resolved atmospherically-broadened lines from small chemical species, while offering the broad tuning range needed to measure broadband spectral features from multiple large chemical species. In this talk the application of this technology for open-path atmospheric measurements will be discussed based on results from laboratory measurements with simulated plumes of chemicals. The performance offered by the system for simultaneous detection of multiple chemical species will be presented.« less

FFT swept filtering: a bias-free method for processing fringe signals in absolute gravimeters

NASA Astrophysics Data System (ADS)

Křen, Petr; Pálinkáš, Vojtech; Mašika, Pavel; Val'ko, Miloš

2018-05-01

Absolute gravimeters, based on laser interferometry, are widely used for many applications in geoscience and metrology. Although currently the most accurate FG5 and FG5X gravimeters declare standard uncertainties at the level of 2-3 μGal, their inherent systematic errors affect the gravity reference determined by international key comparisons based predominately on the use of FG5-type instruments. The measurement results for FG5-215 and FG5X-251 clearly showed that the measured g-values depend on the size of the fringe signal and that this effect might be approximated by a linear regression with a slope of up to 0.030 μGal/mV . However, these empirical results do not enable one to identify the source of the effect or to determine a reasonable reference fringe level for correcting g-values in an absolute sense. Therefore, both gravimeters were equipped with new measuring systems (according to Křen et al. in Metrologia 53:27-40, 2016. https://doi.org/10.1088/0026-1394/53/1/27 applied for FG5), running in parallel with the original systems. The new systems use an analogue-to-digital converter HS5 to digitize the fringe signal and a new method of fringe signal analysis based on FFT swept bandpass filtering. We demonstrate that the source of the fringe size effect is connected to a distortion of the fringe signal due to the electronic components used in the FG5(X) gravimeters. To obtain a bias-free g-value, the FFT swept method should be applied for the determination of zero-crossings. A comparison of g-values obtained from the new and the original systems clearly shows that the original system might be biased by approximately 3-5 μGal due to improperly distorted fringe signal processing.

Demonstration of a Rapidly-Swept External Cavity Quantum Cascade Laser for Atmospheric Sensing Applications

NASA Astrophysics Data System (ADS)

Brumfield, Brian E.; Taubman, Matthew S.; Phillips, Mark C.; Suter, Jonathan D.

2016-06-01

The application of quantum cascade lasers (QCLs) in atmospheric science for trace detection of gases has been demonstrated using sensors in point or remote sensing configurations. Many of these systems utilize single narrowly-tunable (˜10 wn) distributed feedback (DFB-) QCLs that limit simultaneous detection to a restricted number of small chemical species like H2O or N2O. The narrow wavelength range of DFB-QCLs precludes accurate quantification of large chemical species with broad rotationally-unresolved vibrational spectra, such as volatile organic compounds, that play an important role in the chemistry of the atmosphere. External-cavity (EC-) QCL systems are available that offer tuning ranges greater than 100 wn, making them excellent IR sources for measuring multiple small and large chemical species in the atmosphere. While the broad wavelength coverage afforded by an EC system enables measurements of large chemical species, most commercial systems can only be swept over their entire wavelength range at less than 10 Hz. This prohibits broadband simultaneous measurements of multiple chemicals in plumes from natural or industrial sources where turbulence and/or chemical reactivity are resulting in rapid changes in chemical composition on sub-1s timescales. At Pacific Northwest National Laboratory we have developed rapidly-swept EC-QCL technology that acquires broadband absorption spectra (˜100 wn) on ms timescales. The spectral resolution of this system has enabled simultaneous measurement of narrow rotationally-resolved atmospherically-broadened lines from small chemical species, while offering the broad tuning range needed to measure broadband spectral features from multiple large chemical species. In this talk the application of this technology for open-path atmospheric measurements will be discussed based on results from laboratory measurements with simulated plumes of chemicals. The performance offered by the system for simultaneous detection of multiple chemical species will be presented. The Pacific Northwest National Laboratory is operated for the U.S. Department of Energy (DOE) by the Battelle Memorial Institute under Contract No. DE-AC05-76RL01830.

Quantitative light-induced fluorescence technology for quantitative evaluation of tooth wear

NASA Astrophysics Data System (ADS)

Kim, Sang-Kyeom; Lee, Hyung-Suk; Park, Seok-Woo; Lee, Eun-Song; de Josselin de Jong, Elbert; Jung, Hoi-In; Kim, Baek-Il

2017-12-01

Various technologies used to objectively determine enamel thickness or dentin exposure have been suggested. However, most methods have clinical limitations. This study was conducted to confirm the potential of quantitative light-induced fluorescence (QLF) using autofluorescence intensity of occlusal surfaces of worn teeth according to enamel grinding depth in vitro. Sixteen permanent premolars were used. Each tooth was gradationally ground down at the occlusal surface in the apical direction. QLF-digital and swept-source optical coherence tomography images were acquired at each grinding depth (in steps of 100 μm). All QLF images were converted to 8-bit grayscale images to calculate the fluorescence intensity. The maximum brightness (MB) values of the same sound regions in grayscale images before (MB) and phased values after (MB) the grinding process were calculated. Finally, 13 samples were evaluated. MB increased over the grinding depth range with a strong correlation (r=0.994, P<0.001). In conclusion, the fluorescence intensity of the teeth and grinding depth was strongly correlated in the QLF images. Therefore, QLF technology may be a useful noninvasive tool used to monitor the progression of tooth wear and to conveniently estimate enamel thickness.

Depth-encoded dual beam phase-resolved Doppler OCT for Doppler-angle-independent flow velocity measurement

NASA Astrophysics Data System (ADS)

Qian, Jie; Cheng, Wei; Cao, Zhaoyuan; Chen, Xinjian; Mo, Jianhua

2017-02-01

Phase-resolved Doppler optical coherence tomography (PR-D-OCT) is a functional OCT imaging technique that can provide high-speed and high-resolution depth-resolved measurement on flow in biological materials. However, a common problem with conventional PR-D-OCT is that this technique often measures the flow motion projected onto the OCT beam path. In other words, it needs the projection angle to extract the absolute velocity from PR-D-OCT measurement. In this paper, we proposed a novel dual-beam PR-D-OCT method to measure absolute flow velocity without separate measurement on the projection angle. Two parallel light beams are created in sample arm and focused into the sample at two different incident angles. The images produced by these two beams are encoded to different depths in single B-scan. Then the Doppler signals picked up by the two beams together with the incident angle difference can be used to calculate the absolute velocity. We validated our approach in vitro on an artificial flow phantom with our home-built 1060 nm swept source OCT. Experimental results demonstrated that our method can provide an accurate measurement of absolute flow velocity with independency on the projection angle.

Development of advanced stability theory suction prediction techniques for laminar flow control. [on swept wings

NASA Technical Reports Server (NTRS)

Srokowski, A. J.

1978-01-01

The problem of obtaining accurate estimates of suction requirements on swept laminar flow control wings was discussed. A fast accurate computer code developed to predict suction requirements by integrating disturbance amplification rates was described. Assumptions and approximations used in the present computer code are examined in light of flow conditions on the swept wing which may limit their validity.

Recent Progress in Engine Noise Reduction Technologies

NASA Technical Reports Server (NTRS)

Huff, Dennis; Gliebe, Philip

2003-01-01

Highlights from NASA-funded research over the past ten years for aircraft engine noise reduction are presented showing overall technical plans, accomplishments, and selected applications to turbofan engines. The work was sponsored by NASA's Advanced Subsonic Technology (AST) Noise Reduction Program. Emphasis is given to only the engine noise reduction research and significant accomplishments that were investigated at Technology Readiness Levels ranging from 4 to 6. The Engine Noise Reduction sub-element was divided into four work areas: source noise prediction, model scale tests, engine validation, and active noise control. Highlights from each area include technologies for higher bypass ratio turbofans, scarf inlets, forward-swept fans, swept and leaned stators, chevron/tabbed nozzles, advanced noise prediction analyses, and active noise control for fans. Finally, an industry perspective is given from General Electric Aircraft Engines showing how these technologies are being applied to commercial products. This publication contains only presentation vu-graphs from an invited lecture given at the 41st AIAA Aerospace Sciences Meeting, January 6-9, 2003.

Interferometric Near-Infrared Spectroscopy (iNIRS) for determination of optical and dynamical properties of turbid media

PubMed Central

Borycki, Dawid; Kholiqov, Oybek; Chong, Shau Poh; Srinivasan, Vivek J.

2016-01-01

We introduce and implement interferometric near-infrared spectroscopy (iNIRS), which simultaneously extracts optical and dynamical properties of turbid media through analysis of a spectral interference fringe pattern. The spectral interference fringe pattern is measured using a Mach-Zehnder interferometer with a frequency-swept narrow linewidth laser. Fourier analysis of the detected signal is used to determine time-of-flight (TOF)-resolved intensity, which is then analyzed over time to yield TOF-resolved intensity autocorrelations. This approach enables quantification of optical properties, which is not possible in conventional, continuous-wave near-infrared spectroscopy (NIRS). Furthermore, iNIRS quantifies scatterer motion based on TOF-resolved autocorrelations, which is a feature inaccessible by well-established diffuse correlation spectroscopy (DCS) techniques. We prove this by determining TOF-resolved intensity and temporal autocorrelations for light transmitted through diffusive fluid phantoms with optical thicknesses of up to 55 reduced mean free paths (approximately 120 scattering events). The TOF-resolved intensity is used to determine optical properties with time-resolved diffusion theory, while the TOF-resolved intensity autocorrelations are used to determine dynamics with diffusing wave spectroscopy. iNIRS advances the capabilities of diffuse optical methods and is suitable for in vivo tissue characterization. Moreover, iNIRS combines NIRS and DCS capabilities into a single modality. PMID:26832264

A study of the noise radiation from four helicopter rotor blades. [tests in Ames 40 by 20 foot wind tunnel

NASA Technical Reports Server (NTRS)

Lee, A.; Mosher, M.

1978-01-01

Acoustic measurements were taken of a modern helicopter rotor with four blade tip shapes in the NASA Ames 40-by-80-Foot Wind Tunnel. The four tip shapes are: rectangular, swept, trapezoidal, and swept tapered in platform. Acoustic effects due to tip shape changes were studied based on the dBA level, peak noise pressure, and subjective rating. The swept tapered blade was found to be the quietest above an advancing tip Mach number of about 0.9, and the swept blade was the quietest at low speed. The measured high speed impulsive noise was compared with theoretical predictions based on thickness effects; good agreement was found.

Takeoff/approach noise for a model counterrotation propeller with a forward-swept upstream rotor

NASA Technical Reports Server (NTRS)

Woodward, Richard P.; Hall, David G.; Podboy, Gary G.; Jeracki, Robert J.

1993-01-01

A scale model of a counterrotating propeller with forward-swept blades in the forward rotor and aft-swept blades in the aft rotor (designated F39/A31) has been tested in the NASA Lewis 9- by 15-Foot Anechoic Wind Tunnel. This paper presents aeroacoustic results at a takeoff/approach condition of Mach 0.20. Laser Doppler Velocimeter results taken in a plane between the two rotors are also included to quantify the interaction flow field. The intention of the forward-swept design is to reduce the magnitude of the forward rotor tip vortex and/or wakes which impinge on the aft rotor, thus lowering the interaction tone levels.

Frequency-agile gyrotron for electron decoupling and pulsed dynamic nuclear polarization.

PubMed

Scott, Faith J; Saliba, Edward P; Albert, Brice J; Alaniva, Nicholas; Sesti, Erika L; Gao, Chukun; Golota, Natalie C; Choi, Eric J; Jagtap, Anil P; Wittmann, Johannes J; Eckardt, Michael; Harneit, Wolfgang; Corzilius, Björn; Th Sigurdsson, Snorri; Barnes, Alexander B

2018-04-01

We describe a frequency-agile gyrotron which can generate frequency-chirped microwave pulses. An arbitrary waveform generator (AWG) within the NMR spectrometer controls the microwave frequency, enabling synchronized pulsed control of both electron and nuclear spins. We demonstrate that the acceleration of emitted electrons, and thus the microwave frequency, can be quickly changed by varying the anode voltage. This strategy results in much faster frequency response than can be achieved by changing the potential of the electron emitter, and does not require a custom triode electron gun. The gyrotron frequency can be swept with a rate of 20 MHz/μs over a 670 MHz bandwidth in a static magnetic field. We have already implemented time-domain electron decoupling with dynamic nuclear polarization (DNP) magic angle spinning (MAS) with this device. In this contribution, we show frequency-swept DNP enhancement profiles recorded without changing the NMR magnet or probe. The profile of endofullerenes exhibits a DNP profile with a <10 MHz linewidth, indicating that the device also has sufficient frequency stability, and therefore phase stability, to implement pulsed DNP mechanisms such as the frequency-swept solid effect. We describe schematics of the mechanical and vacuum construction of the device which includes a novel flanged sapphire window assembly. Finally, we discuss how commercially available continuous-wave gyrotrons can potentially be converted into similar frequency-agile high-power microwave sources. Copyright © 2018. Published by Elsevier Inc.

Downstream influence of swept slot injection in hypersonic turbulent flow

NASA Technical Reports Server (NTRS)

Hefner, J. N.; Cary, A. M., Jr.; Bushnell, D. B.

1977-01-01

Results of an experimental and numerical investigation of tangential swept slot injection into a thick turbulent boundary layer at Mach 6 are presented. Film cooling effectiveness, skin friction, and flow structure downstream of the swept slot injection were investigated. The data were compared with that for unswept slots, and it was found that cooling effectiveness and skin friction reductions are not significantly affected by sweeping the slot.

Aerodynamic Classification of Swept-Wing Ice Accretion

NASA Technical Reports Server (NTRS)

Broeren, Andy; Diebold, Jeff; Bragg, Mike

2013-01-01

The continued design, certification and safe operation of swept-wing airplanes in icing conditions rely on the advancement of computational and experimental simulation methods for higher fidelity results over an increasing range of aircraft configurations and performance, and icing conditions. The current state-of-the-art in icing aerodynamics is mainly built upon a comprehensive understanding of two-dimensional geometries that does not currently exist for fundamentally three-dimensional geometries such as swept wings. The purpose of this report is to describe what is known of iced-swept-wing aerodynamics and to identify the type of research that is required to improve the current understanding. Following the method used in a previous review of iced-airfoil aerodynamics, this report proposes a classification of swept-wing ice accretion into four groups based upon unique flowfield attributes. These four groups are: ice roughness, horn ice, streamwise ice, and spanwise-ridge ice. For all of the proposed ice-shape classifications, relatively little is known about the three-dimensional flowfield and even less about the effect of Reynolds number and Mach number on these flowfields. The classifications and supporting data presented in this report can serve as a starting point as new research explores swept-wing aerodynamics with ice shapes. As further results are available, it is expected that these classifications will need to be updated and revised.