Sample records for swine effluent application

  1. Forms of phosphorus transfer in runoff under no-tillage in a soil treated with successive swine effluents applications.

    PubMed

    Lourenzi, Cledimar Rogério; Ceretta, Carlos Alberto; Tiecher, Tadeu Luis; Lorensini, Felipe; Cancian, Adriana; Stefanello, Lincon; Girotto, Eduardo; Vieira, Renan Costa Beber; Ferreira, Paulo Ademar Avelar; Brunetto, Gustavo

    2015-04-01

    Successive swine effluent applications can substantially increase the transfer of phosphorus (P) forms in runoff. The aim of this study was to evaluate P accumulation in the soil and transfer of P forms in surface runoff from a Hapludalf soil under no-tillage subjected to successive swine effluent applications. This research was carried out in the Agricultural Engineering Department of the Federal University of Santa Maria, Brazil, from 2004 to 2007, on a Typic Hapludalf soil. Swine effluent rates of 0, 20, 40, and 80 m3 ha(-1) were broadcast over the soil surface prior to sowing of different species in a crop rotation. Soil samples were collected in stratified layers, and the levels of available P were determined. Samples of water runoff from the soil surface were collected throughout the period, and the available, soluble, particulate, and total P were measured. Successive swine effluent applications led to increases in P availability, especially in the soil surface, and P migration through the soil profile. Transfer of P forms was closely associated with runoff, which is directly related to rainfall volume. Swine effluent applications also reduced surface runoff. These results show that in areas with successive swine effluent applications, practices that promote higher water infiltration into the soil are required, e.g., crop rotation and no-tillage system.

  2. Corn response to long-term applications of cattle manure, swine effluent, and inorganic nitrogen fertilizer

    USDA-ARS?s Scientific Manuscript database

    Cattle (Bos taurus) manure and swine (Sus scrofa) effluent are applied to cropland to recycle nutrients, build soil quality, and increase crop productivity. The objective of this study was to determine the long-term effects of land application of cattle manure and swine effluent using the Kansas Nut...

  3. Tetracycline resistance in semi-arid agricultural soils under long-term swine effluent application.

    PubMed

    Popova, Inna E; Josue, Rosemarie D R; Deng, Shiping; Hattey, Jeffory A

    2017-05-04

    Annually, millions pounds of antibiotics are released unmetabolized into environment along with animal wastes. Accumulation of antibiotics in soils could potentially induce the persistence of antibiotic resistant bacteria. Antibiotics such as tetracyclines and tetracycline-resistant bacteria have been previously detected in fields fertilized with animal manure. However, little is known about the accumulation of tetracyclines and the development of tetracycline resistance in semi-arid soils. Here we demonstrate that continuous land application with swine effluent, containing trace amounts of chlortetracycline, does not necessarily induce tetracycline resistance in soil bacteria. Based on the testing of more than 3,000 bacteria isolated from the amended soils, we found no significant increase in the occurrence and level of chlortetracycline resistant bacteria in soils after 15 years of continuous swine effluent fertilization. To account for a possible transfer of tetracycline-resistant bacteria originated from the swine effluent to soils, we analyzed two commonly found tetracycline resistant genes, tet(O) and tet(M), in the swine effluent and fertilized soils. Both genes were present in the swine effluent, however, they were not detectable in soils applied with swine effluent. Our data demonstrate that agronomic application of manure from antibiotic treated swine effluent does not necessarily result in the development of antibiotic bacterial resistance in soils. Apparently, concentrations of chlortetracycline present in manure are not significant enough to induce the development of antibiotic bacterial resistance.

  4. Changes in soil nutrients after 10 years of cattle manure and swine effluent application

    USDA-ARS?s Scientific Manuscript database

    Application of cattle manure and swine effluent to cropland builds nutrient pools, affects soil quality, and increases crop productivity. The objective of the present study was to evaluate the rate of change in soil nutrient concentration and soil chemical properties due to cattle manure and swine e...

  5. Nutrient removal from swine lagoon effluent by duckweed

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bergmann, B.A.; Cheng, J.; Classen, J.

    2000-04-01

    Three duckweed geographic isolates were grown on varying concentrations of swine lagoon effluent in a greenhouse to determine their ability to remove nutrients from the effluent. Duckweed biomass was harvested every other day over a 12-day period. Duckweed biomass production, nutrient loss from the swine lagoon effluent, and nutrient content of duckweed biomass were used to identify effluent concentrations/geographic isolate combinations that are effective in terms of nutrient utilization from swine lagoon effluent and production of healthy duckweed biomass. When Lemna minor geographic isolate 8627 was grown on 50% swine lagoon effluent, respective losses of TKN, NH{sub 3}-N, TP, OPO{submore » 4}-P, TOC, K, Cu, and Zn were 83, 100, 49, 31, 68, 21, 28 and 67%.« less

  6. Effect of manure application rate and timing on the leaching potential of antibiotic resistant bacteria.

    USDA-ARS?s Scientific Manuscript database

    Antibiotics are used in swine production for therapeutic and growth promotion purposes. Because land application is the most common method of disposing of swine lagoon effluent, there exists the potential threat of contaminating the underlying groundwater with antimicrobial-resistant bacteria (ARB) ...

  7. Corn grain and nutrient uptake response to different swine manure application methods

    USDA-ARS?s Scientific Manuscript database

    Farmers are looking for better management practices to enhance production and reduce negative environmental impact from nitrogen (N) fertilizer application since N is one of the most important and costly nutrient inputs for crop production. In this field experiment pre-plant swine effluent applicati...

  8. Reducing phosphorus in swine effluent with aluminum chloride treatment during lagoon cleanout

    USDA-ARS?s Scientific Manuscript database

    Phosphorus (P) runoff from agricultural lands fertilized with swine manure can be a significant environmental issue. The objective of this study was to evaluate the effect of aluminum chloride (AlCl3) applications to a swine lagoon during total clean out on P concentrations in manure and runoff wat...

  9. 40 CFR 412.40 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CONCENTRATED ANIMAL FEEDING OPERATIONS (CAFO) POINT SOURCE CATEGORY Swine, Poultry, and Veal Calves § 412.40...) under 40 CFR 122.23 and includes the following animals: swine; chickens; turkeys; and veal calves. This...

  10. 40 CFR 412.40 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CONCENTRATED ANIMAL FEEDING OPERATIONS (CAFO) POINT SOURCE CATEGORY Swine, Poultry, and Veal Calves § 412.40...) under 40 CFR 122.23 and includes the following animals: swine; chickens; turkeys; and veal calves. This...

  11. Settling and survival profile of enteric pathogens in the swine effluent for water reuse purpose.

    PubMed

    Fongaro, G; Kunz, A; Magri, M E; Schissi, C D; Viancelli, A; Philippi, L S; Barardi, C R M

    2016-11-01

    The present study evaluated the pathogens persistence and settling profile in swine effluent. We determined the enteric pathogens settling characteristics, their survival and inactivation profile in swine effluent (for water reuse purpose) and in sludge (generated after aerobic treatment - during secondary settling process). The study was performed in laboratorial-scale and in full-scale (manure treatment plant). Enteric viruses and enteric bacteria were used as biomarkers. Results showed that these enteric pathogens were significantly reduced from swine effluent during secondary settling process, and enteric viruses removal was correlated with the suspended solids decantation. The design of secondary settlers can be adapted to improve pathogens removal, by diminishing the solids loading rate per area and time, ending in higher hydraulic retention times. Copyright © 2016 Elsevier GmbH. All rights reserved.

  12. Utility of specific biomarkers to assess safety of swine manure for biofertilizing purposes.

    PubMed

    Fongaro, G; Viancelli, A; Magri, M E; Elmahdy, E M; Biesus, L L; Kich, J D; Kunz, A; Barardi, C R M

    2014-05-01

    Swine production is an important economic activity in Brazil, and there is interest in the development of clean production mechanisms to support sustainable agro-industrial activities. The biomass derived from swine manure has good potential to be used as a biofertilizer due to its high nutrient concentration. However, the land application of manure should be based on safety parameters such as the presence of pathogens that can potentially infect animals and people. This study was designed to assess the presence of porcine circovirus-2 (PCV2), porcine adenovirus (PAdV), rotavirus-A (RV-A) and Salmonella spp. in liquid manure, as well the infectivity of two genotypes of circovirus-2 (PCV2a and PCV2b) present in liquid manure. Three swine farms were evaluated: 1) a nursery production farm (manure analyzed before and after anaerobic biodigestion), 2) a grow-finish production farm (analyzed before and after anaerobic biodigestion), and 3) a second grow-finish production farm (raw manure-affluent). PCV2, PAdV and RV-A were present before and after anaerobic biodigestion (either affluent or effluent) at all farms. Salmonella spp. were detected at farm 1 (affluent and effluent) and farm 3 (raw manure-affluent) but not farm 2 (affluent and effluent). When the ability of the anaerobic biodigestion process to reduce viral concentration was evaluated, no significant reduction was observed (P>0.05). Both the PCV2a and PCV2b genotypes were detected, suggesting viral co-infection in swine production. The results revealed infectious PCV2 even after anaerobic biodigestion treatment. The presence of Salmonella spp. and enteric viruses, especially infectious PCV2, in the final effluent from the anaerobic biodigester system suggests that the process is inefficient for pathogen inactivation. Due to the prevalence and infectivity of PCV2 and considering the successful use of molecular methods coupled to cell culture for detecting infectious PCV2, we suggest that this virus can be used as a bioindicator in swine manure treatment systems to check the efficiency of pathogen inactivation and ensure the production of safe biofertilizers from swine manure. Copyright © 2014 Elsevier B.V. All rights reserved.

  13. Nitrogen source and application method impact on corn yield and nutrient uptake

    USDA-ARS?s Scientific Manuscript database

    Farmers are looking for better management practices to enhance production and reduce negative environmental impact from nitrogen (N) fertilizer application since N is one of the most important and costly nutrient inputs for crop production. In this field experiment pre-plant swine effluent applicati...

  14. Source analysis of organic matter in swine wastewater after anaerobic digestion with EEM-PARAFAC.

    PubMed

    Zeng, Zhuo; Zheng, Ping; Ding, Aqiang; Zhang, Meng; Abbas, Ghulam; Li, Wei

    2017-03-01

    Swine wastewater is one of the most serious pollution sources, and it has attracted a great public concern in China. Anaerobic digestion technology is extensively used in swine wastewater treatment. However, the anaerobic digestion effluents are difficult to meet the discharge standard. The results from batch experiments showed that plenty of refractory organic matter remained in the effluents after mesophilic anaerobic digestion for 30 days. The effluent total COD (tCOD) and soluble COD (sCOD) were 483 and 324 mg/L, respectively, with the sCOD/tCOD ratio of 0.671. Fluorescence excitation-emission matrix (EEM) coupled with parallel factor analysis (PARAFAC) revealed that the dissolved organic matter in the effluents was tryptophan-like substance, humic acid substance, and fulvic acid substance. Based on the appearance time during anaerobic digestion, tryptophan-like substance and humic acid substance were inferred to originate from the raw swine wastewater, and the fulvic acid substance was inferred to be formed in the anaerobic digestion. This work has revealed the source of residual organic matter in anaerobic digestion of swine wastewater and has provided some valuable information for the post-treatment.

  15. Die-off rates of Cryptosporidium parvum oocysts in a swine lagoon and in a spray field

    USDA-ARS?s Scientific Manuscript database

    Background: Because of several large-scale outbreaks of cryptosporidiosis in humans, Cryptosporidium has become a public health concern. Commercial swine operations apply large volumes of effluent from lagoons to spray fields as a waste management practice. This effluent is a source of Cryptosporidi...

  16. New concepts of ammonia removal from digested swine effluents using anammox based deammonification process

    USDA-ARS?s Scientific Manuscript database

    Production of biogas from swine manure using anaerobic digesters (AD) is projected to be important in the future. However, surplus nitrogen (N) in AD effluents is difficult to remove using current technology (nitrification/denitrification) because low carbon availability after biogas production. W...

  17. Performance of two swine manure treatment systems on chemical composition and on the reduction of pathogens.

    PubMed

    Viancelli, A; Kunz, A; Steinmetz, R L R; Kich, J D; Souza, C K; Canal, C W; Coldebella, A; Esteves, P A; Barardi, C R M

    2013-01-01

    Swine effluents must be correctly handled to avoid negative environmental impacts. In this study, the profiles of two swine manure treatment systems were evaluated: a solid-liquid separation step, followed by an anaerobic reactor, and an aerobic step (System 1); and a biodigester followed by serial lagoons (System 2). Both systems were described by the assessment of chemical, bacterial and viral parameters. The results showed that in System 1, there was reduction of chemicals (COD, phosphorus, total Kjeldhal nitrogen - TKN - and NH(3)), total coliforms and Escherichia coli; however, the same reduction was not observed for Salmonella sp. Viral particles were significantly reduced but not totally eliminated from the effluent. In System 2, there was a reduction of chemicals, bacteria and viruses with no detection of Salmonella sp., circovirus, parvovirus, and torque teno virus in the effluent. The chemical results indicate that the treated effluent can be reused for cleaning swine facilities. However, the microbiological results show a need of additional treatment to achieve a complete inactivation for cases when direct contact with animals is required. Copyright © 2012 Elsevier Ltd. All rights reserved.

  18. Molecular Characterization of Swine Manure Lagoon Microbial and Antibiotic Resistant Populations

    USDA-ARS?s Scientific Manuscript database

    Background: The differences in swine manure lagoon effluent based on differing management styles or approaches such as different stages of swine rearing determines the presence of variable antibiotic resistance determinants and functional microbial populations. These concerns determine the suitabil...

  19. Using broiler and swine manure lagoon effluent in sawdust-based swine mortality composts: effects of nutrients, bacteria, and gaseous emissions

    USDA-ARS?s Scientific Manuscript database

    Disposition of mortalities challenges confined animal feeding operations (CAFOs) especially sow (farrowing) farms, which experience mortalities daily. Environmental regulations and high transportation costs may preclude incineration, landfill burial, and rendering; therefore, swine CAFOs in Mississ...

  20. Assessment of nutrients, bacteria, and gaseous emissions in swine mortality composts of sawdust, broiler litter, and swine lagoon effluent

    USDA-ARS?s Scientific Manuscript database

    Disposition of mortalities challenges confined animal feeding operations (CAFOs), especially large sow (farrowing) farms, which experience mortalities daily. Regulations preclude incineration and high costs make rendering impractical. Swine CAFOs in the Mid-South US practice mortality composting w...

  1. Effects of duckweed (Spriodela polyrrhiza) remediation on the composition of dissolved organic matter in effluent of scale pig farms.

    PubMed

    Li, Lei; Liu, Ming; Wu, Meng; Jiang, Chunyu; Chen, Xiaofen; Ma, Xiaoyan; Liu, Jia; Li, Weitao; Tang, Xiaoxue; Li, Zhongpei

    2017-05-01

    The swine effluent studied was collected from scale pig farms, located in Yujiang County of Jiangxi Province, China, and duckweed (Spriodela polyrrhiza) was selected to dispose the effluent. The purpose of this study was to elucidate the effects of duckweed growth on the dissolved organic matter composition in swine effluent. Throughout the experiment period, the concentrations of organic matter were determined regularly, and the excitation-emission matrix (3DEEM) spectroscopy was used to characterize the fluorescence component. Compared with no-duckweed treatments (controls), the specific ultra-violet absorbance at 254nm (SUVA 254 ) was increased by a final average of 34.4% as the phytoremediation using duckweed, and the removal rate of DOC was increased by a final average of 28.0%. In swine effluent, four fluorescence components were identified, including two protein-like (tryptophan, tyrosine) and two humic-like (fulvic acids, humic acids) components. For all treatments, the concentrations of protein-like components decreased by a final average of 69.0%. As the growth of duckweed, the concentrations of humic-like components were increased by a final average of 123.5% than controls. Significant and positive correlations were observed between SUVA 254 and humic-like components. Compared with the controls, the humification index (HIX) increased by a final average of 9.0% for duckweed treatments. Meanwhile, the duckweed growth leaded to a lower biological index (BIX) and a higher proportion of microbial-derived fulvic acids than controls. In conclusion, the duckweed remediation not only enhanced the removal rate of organic matter in swine effluent, but also increased the percent of humic substances. Copyright © 2016. Published by Elsevier B.V.

  2. Ammonia and greenhouse gas emissions in swine mortality composts of sawdust, broiler litter, and swine lagoon effluent

    USDA-ARS?s Scientific Manuscript database

    Animal agriculture is looking for innovative means to dispose of mortalities. Composting is an environmentally friendly option that retains the nutrients of the animal and organic materials. Southern U.S. swine farrowing operations often use sawdust as a C source for mortality composting. The obje...

  3. Using full-scale duckweed ponds as the finish stage for swine waste treatment with a focus on organic matter degradation.

    PubMed

    Mohedano, R A; Costa, R H R; Hofmann, S M; Belli Filho, P

    2014-01-01

    The rapid increase in the number of swine has caused pronounced environmental impacts worldwide, especially on water resources. As an aggregate, smallholdings have an important role in South American pork production, contributing to the net diffusion of pollution. Thus, duckweed ponds have been successfully used for swine waste polishing, mainly for nutrient removal. Few studies have been carried out to assess organic matter degradation in duckweed ponds. Hence, the present study evaluated the efficiency of two full-scale duckweed ponds for organic matter reduction of swine waste on small pig farms. Duckweed ponds, in series, received the effluent after an anaerobic biodigester and storage pond, with a flow rate of 1 m(3) day(-1). After 1 year of monitoring, an improvement in effluent quality was observed, with a reduction in biochemical oxygen demand (BOD) and total chemical oxygen demand (tCOD), respectively, of 94.8 and 96.7%, operating at a loading rate of approximately 27 kgBOD ha(-1) day(-1) and 131 kgCOD ha(-1) day(-1). Algae inhibition due to duckweed coverage was strongly observed in the pond effluent, where chlorophyll a and turbidity remained below 25 μg L(-1) and 10 NTU. Using the study conditions described herein, duckweed ponds were shown to be a suitable technology for swine waste treatment, contributing to the environmental sustainability of rural areas.

  4. Occurrence and sources of antibiotics and their metabolites in river water, WWTPs, and swine wastewater in Jiulongjiang River basin, south China.

    PubMed

    Jiang, Hongyou; Zhang, Dandan; Xiao, Shichang; Geng, Chunnv; Zhang, Xian

    2013-12-01

    In this study, the occurrence and sources of five cataloged antibiotics and metabolites were studied in Jiulongjiang River basin, south China. Nineteen antibiotics and 13 metabolites were detected in water samples from 16 river sampling sites, wastewater from 5 swine-raising facilities, and effluent from 5 wastewater treatment plants (WWTPs). The results showed that 12 antibiotics and 6 metabolites were detected in river water samples. Sulfonamides (SAs) and their metabolites were detected at high concentrations (8.59-158.94 ng/L). Tetracyclines (TCs) and their metabolites were frequently detected in swine wastewater, and the maximum concentration was up to the level in milligram per liter. Macrolides (MLs) and β-lactams (β-Ls) were found in all WWTP effluent samples and some river samples, while they were never found in any of the swine wastewater samples. SAs and quinolones (QNs) were detected in all samples. Hierarchical cluster analysis of 16 surface water samples was applied to achieve the spatial distribution characteristics of antibiotics in the Jiulongjiang River. As a result, two categories were obviously obtained. Principal component analysis and redundancy analysis showed that TCs and SAs as well as their metabolites were the major antibiotics in Jiulongjiang River, and they mainly originated from swine wastewater, while the QNs, MLs, and β-Ls in the Jiulongjiang River came from WWTP effluent.

  5. Influence of solid-liquid separation strategy on biogas yield from a stratified swine production system.

    PubMed

    Cestonaro do Amaral, André; Kunz, Airton; Radis Steinmetz, Ricardo Luis; Scussiato, Lucas Antunes; Tápparo, Deisi Cristina; Gaspareto, Taís Carla

    2016-03-01

    As the fourth largest swine producer and exporter, Brazil has increased its participation in the global swine production market. Generally, these units concentrate a large number of animals and generate effluents that must be correctly managed to prevent environmental impacts, being anaerobic digestion is an interesting alternative for treating these effluents. The low-volatile solid concentration in the manure suggests the need for solid-liquid separation as a tool to improve the biogas generation capacity. This study aimed to determine the influence of simplified and inexpensive solid-liquid separation strategies (screening and settling) and the different manures produced during each swine production phase (gestating and farrowing sow houses, nursery houses and finishing houses) on biogas and methane yield. We collected samples in two gestating sow houses (GSH-a and GSH-b), two farrowing sow houses (FSH-a and FSH-b), a nursery house (NH) and a finishing house (FH). Biochemical methane potential (BMP) tests were performed according to international standard procedures. The settled sludge fraction comprised 20-30% of the raw manure volume, which comprises 40-60% of the total methane yield. The methane potential of the settled sludge fraction was approximately two times higher than the methane potential of the supernatant fraction. The biogas yield differed among the raw manures from different swine production phases (GSH-a 326.4 and GSH-b 577.1; FSH-a 860.1 and FSH-b 479.2; NH -970.2; FH 474.5 NmLbiogas.gVS(-1)). The differences were relative to the production phase (feed type and feeding techniques) and the management of the effluent inside the facilities (water management). Brazilian swine production has increased his participation in the global market, been the fourth producer and the fourth exporter. The segregation of swine production in multiple sites has increased its importance, due to the possibilities to have more specialized units. Generally, these units concentrate a large number of animals and generate effluents that must be correctly managed to avoid environmental impact. Due to the biodegradability of manure, anaerobic digestion is an interesting alternative to treat these effluents. The low volatile solid concentration in the swine manure suggests the need for solid-liquid separation as a tool to improve biogas generation capacity. The present study aimed to determine the influence of simplified and cheap solid-liquid separation strategies (based on screening and settling) and different manure of each swine production phases (gestating and farrowing sows houses, nursery houses and finishing houses) on biogas and methane yield. We collected samples in two gestating sows house (GSH-a and GSH-b), two farrowing sows house (FSH-a and FSH-b), a nursery house (NH) and a finishing house (FH). The Biochemical Methane Production (BMP) tests were performed according to international standard procedure (VDI 4630). The settled sludge fraction responds for 20-30% of raw manure volume, producing 40-60% of the total methane yield. The methane potential of settled sludge fraction was about 2 times higher than the supernatant fraction. There are differences on biogas yield between the raw manure of different swine production phases (GSH-a 326.4 and GSH-b 577.1; FSH-a 860.1 and FSH-b 479.2; NH 970.2; FH 474.5 NmLbiogas.gVS(-1)). The differences are relative to production phase (feed type, feeding techniques, etc.), but also the management of the effluent inside the facilities (water management). Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Using broiler litter and swine manure lagoon effluent in sawdust-based swine mortality composts: Effects on nutrients, bacteria, and gaseous emissions.

    PubMed

    McLaughlin, M R; Brooks, J P; Adeli, A; Miles, D M

    2015-11-01

    Disposition of mortalities challenges confined animal feeding operations (CAFOs), especially sow (farrowing) farms, which experience mortalities daily. Regulations and transportation costs may preclude incineration, landfill burial, and rendering; therefore, swine CAFOs in Mississippi in the Mid-South U.S. often compost mortalities. In this study, a farm-standard composting mix of sawdust (S) and water (W) was compared with mixes where N was supplied by broiler litter (L) and water was replaced with swine lagoon effluent (E). The objective was to assess the effects of these manure byproducts: 1) on nutrients and bacteria in composts destined for land application; and 2) on emissions of ammonia and greenhouse gases. Three replications of four mixes (SW, SLW, SE, SLE) were compared in microcosms comprising modified plastic recycling bins. The experiment was repeated three times in different seasons in one year. Mixes were compared for differences in temperature, water content, nutrients (C, N, P, K, Ca, Mg, Na, Mn, Fe, Cu, Zn), bacteria (Gram-, Gram+, Clostridium perfringens, Salmonella, Listeria, Escherichia coli), and emissions (NH3, CO2, CH4, N2O). Litter addition increased composting temperatures initially and after aerations; increased nutrient concentrations, except C, in start mixes and all except C and N, in finish mixes; increased Gram+ bacteria, Salmonella, and E. coli in start mixes, but only Gram+s in finish mixes; and increased emissions. Effluent addition increased early composting temperatures; had no effect on nutrients or bacteria, except increased C. perfringens in start, but not finish mixes; and had no effect on emissions. Nutrients in finish composts did not differ among mixes for N (average 3.3%), but litter composts had more P and K, and lower N:P than composts without litter. Improving mortality composting is of global importance as increasing livestock populations and intensive animal production systems require practical, safe, environmentally sound disposal of carcasses. Published by Elsevier B.V.

  7. ANALYSIS OF SWINE LAGOONS AND GROUND WATER FOR ENVIRONMENTAL ESTROGENS

    EPA Science Inventory

    A method was developed for analysis of low levels of natural (estradiol, estrone, estriol) and synthetic (ethynylestradiol) estrogens in ground water and swine waste lagoon effluent. The method includes solid phase extraction of the estrogens, preparation of pentafluorobenzyl der...

  8. ANALYSIS OF SWINE LAGOONS AND GROUND WATER FOR ENVIRONMENTAL ESTROGENS

    EPA Science Inventory

    A method was developed for analysis of low levels of natural (estradiol, estrone, estriol) and synthetic (ethinyl estradiol) estrogens in ground water and swine waste lagoon effluent. The method includes solid phase extraction of the estrogens, preparation of pentafluorobenzyl de...

  9. Recovery of ammonia and phosphate minerals from swine wastewater using gas-permeable membranes

    USDA-ARS?s Scientific Manuscript database

    Gas-permeable membrane technology is useful to recover ammonia from liquid manures. In this study, phosphorus (P) recovery via magnesium chloride precipitation was enhanced by combining it with ammonia recovery through gas-permeable membranes. Anaerobically digested swine effluent containing approx...

  10. Solids and nutrient removal from flushed swine manure using polyacrylamides

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Vanotti, M.B.; Hunt, P.G.

    1999-12-01

    Most of the organic nutrients and reduced carbon (C) materials in liquid swine manure are contained in fine suspended particles that are not separated by available mechanical separators. Treatment with polyacrylamide (PAM) polymers prior to mechanical removal or gravity settling has the potential for enhancing solids-liquid separation, thus concentrating nitrogen (N), phosphorus (P), and organic C. In this work, the authors determined PAM charge and density characteristics most desirable for swine wastewater applications and established the optimum chemical requirement. Treatments were applied to flushed manure from two swine operations in North Carolina. Cationic PAMs significantly increased solids separation while performancemore » of neutral and anionic types was not different from a control. Cationic PAMs with moderate-charge density (20%) were more effective than polymers with higher charge density. Flocs were large and effectively retained with a 1-mm screen. Optimum PAM rate varied with the amount of total suspended solids (TSS) in the liquid manure; 26 and 79 mg PAM/L for samples containing 1.5 and 4.1 g TSS/L, respectively. Corresponding TSS removal efficiencies were 90 to 94%. In contrast, screening without PAM treatment captured only 5 to 14% of the suspended solids. Polymer usage rate was consistent and averaged 2.0{degree} based on weight of dry solids produced. Volatile suspended solids (VSS) were highly correlated with TSS and comprised 79.5% of TSS. Chemical oxygen demand (COD) and organic nutrient concentrations in the effluent were also significantly decreased by PAM treatment. The decrease of COD concentration, an important consideration for odor control, was linearly related with removal of suspended solids, at a rate of 2.0 g COD/g TSS and 2.6 g COD/g VSS. Removal efficiency of organic N and P followed approximately a 1:1 relationship with removal efficiency of TSS. Chemical cost to capture 90% of the suspended solids was estimated to be $0.026 per hog per day ($2.79 per finished hog). Results obtained indicate that PAM treatment is very effective for removal of manure solids, COD, and organic nutrients from flushed swine effluents. The technology provides an attractive alternative to existing liquid manure handling methods for conserving nutrients and avoiding excessive nutrient application in areas where swine production is concentrated.« less

  11. Effective swine wastewater treatment by combining microbial fuel cells with flocculation.

    PubMed

    Ding, Weijun; Cheng, Shaoan; Yu, Liliang; Huang, Haobin

    2017-09-01

    Microbial fuel cells (MFCs) provide a cost-effective method for treating swine wastewater treatment and simultaneously producing electricity, yet they need to be combined with other wastewater treatment processes to improve the effluent water quality. In this paper, we constructed single-chamber air-cathode MFCs with a compact configuration for nitrogen and COD removal and high electricity production and combined them with a low-cost flocculation process to discharge higher quality wastewater. We show that MFCs could remove ammonia at a rate of 269.2 ± 0.5 g m -3 d -1 (99.1± 0.1% ammonia removal efficiency) with a maximum power density of 37.5 W m -3 and 21.6% of coulombic efficiency at a 40:60 ratio of raw swine wastewater to denitrification effluent of swine wastewater. Up to 82.5 ± 0.5% COD could be removed with MFCs, from 2735 ± 15 mg L -1 to 480 ± 15 mg L -1 , and flocculation further reduced levels to 90 ± 1 mg L -1 for a 96.6 ± 0.2% overall COD removal efficiency of the combination technology. Cost analysis of the combined MFC and flocculation process showed a net economic benefit of $ 0.026 m -3 . In summary, this novel combination wastewater treatment method provides an effective way to treat swine wastewater to low pollutant levels in the effluent at low cost (a net gain). Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. The fate of crop nutrients during digestion of swine manure in psychrophilic anaerobic sequencing batch reactors.

    PubMed

    Massé, D I; Croteau, F; Masse, L

    2007-11-01

    The objectives of the study were to measure the levels of manure nutrients retained in psychrophilic anaerobic sequencing batch reactors (PASBRs) digesting swine manure, and to determine the distribution of nutrients in the sludge and supernatant zones of settled bioreactor effluent. Anaerobic digestion reduced the total solids (TS) concentration and the soluble chemical oxygen demand (SCOD) of manure by 71.4% and 79.9%, respectively. The nitrogen, potassium, and sodium fed with the manure to the PASBRs were recovered in the effluent. The bioreactors retained on average 25.5% of the P, 8.7% of the Ca, 41.5% of the Cu, 18.4% of the Zn, and 67.7% of the S fed to the PASBRs. The natural settling of bioreactor effluent allowed further nutrient separation. The supernatant fraction, which represented 71.4% of effluent volume, contained 61.8% of the total N, 67.1% of the NH4-N, and 73.3% of the Na. The settled sludge fraction, which represented 28.6% of the volume, contained 57.6% of the solids, 62.3% of the P, 71.6% of the Ca, 89.6% of the Mg, 76.1% of the Al, 90.0% of the Cu, 74.2% of the Zn, and 52.2% of the S. The N/P ratio was increased from 3.9 in the raw manure to 5.2 in the bioreactor effluent and 9.2 in the supernatant fraction of the settled effluent. The PASBR technology will then substantially decrease the manure management costs of swine operations producing excess phosphorus, by reducing the volume of manure to export outside the farm. The separation of nutrients will also allow land spreading strategies that increase the agronomic value of manure by matching more closely the crop nutrient requirements.

  13. Short-term and seasonal soil nitrogen dynamics and recovery by bermudagrass irrigated with 15N labeled swine lagoon effluent

    USDA-ARS?s Scientific Manuscript database

    The experiment was conducted at a commercial swine operation located in Lowndes County, Mississippi in an acid Vaiden silty clay (very fine, montmorillonitic, thermic Vertic Hapludalf) used in this study is representative of the Alabama and Mississippi Blackland Prairie major land resource area. A ...

  14. Recovery of ammonia and production of high-grade phosphates from digester effluents

    USDA-ARS?s Scientific Manuscript database

    Conservation and recovery of nitrogen and phosphorus from animal wastes and municipal effluents is important because of economic and environmental reasons. In this paper we present a novel technology for separation and recovery of ammonia and phosphorus from liquid swine manure. Phosphorus recovery ...

  15. Pre-digestion to enhance volatile fatty acids (VFAs) concentration as a carbon source for denitrification in treatment of liquid swine manure.

    PubMed

    Wu, Sarah Xiao; Chen, Lide; Zhu, Jun; Walquist, McKenzie; Christian, David

    2018-04-30

    Insufficient denitrification in biological treatment is often a result of the lack of a carbon source. In this study, use of the volatile fatty acids (VFAs) generated via pre-digestion as a carbon source to improve denitrification in sequencing batch reactor (SBR) treatment of liquid swine manure was investigated. The pre-digestion of swine manure was realized by storing the manure in a sealed container in room temperature and samples were taken periodically from the container to determine the VFA levels. The results showed that after 14 days of pre-digestion, the VFA level in the digested liquid was increased by 200%. A polynomial relationship for the VFA level in the digested manure with the digestion time was observed with a correlation coefficient being 0.9748. Two identical SBRs were built and operated on 8-h cycles in parallel, with one fed with pre-digested and the other raw swine manure. There were five phases included in each cycle, i.e., anaerobic (90 min), anoxic (150 min), anoxic/anaerobic (90 min), anoxic/aerobic (120 min), and settle/decant (30 min), and the feeding was split to 600 mL/200 mL and performed at the beginning of and 240 min into the cycle. The SBR fed on pre-digested swine manure achieved successful denitrification with only 0.35 mg/L nitrate left in the effluent, compared to 15.9 mg/L found in the effluent of the other SBR. Nitrite was not detected in the effluent from both SBRs. The results also indicated that there was no negative impact of feeding SBRs with the pre-digested liquid swine manure for treatment on the removal of other constituents such as total solids (TS), volatile solids (VS), suspended solids (SS), volatile suspended solids (VSS), and soluble chemical oxygen demand (COD). Therefore, anaerobic digestion as a pretreatment can be an effective way to condition liquid swine manure for SBR treatment to achieve sufficient nitrate removal.

  16. [Isolation, Identification and Characteristic Analysis of an Oil-producing Chlorella sp. Tolerant to High-strength Anaerobic Digestion Effluent].

    PubMed

    Yang, Chuang; Wang, Wen-guo; Ma, Dan-wei; Tang, Xiao-yu; Hu, Qi-chun

    2015-07-01

    A Chlorella strain tolerant to high-strength anaerobic digestion effluent was isolated from the anaerobic digestion effluent with a long-term exposure to air. The strain was identified as a Chlorella by morphological and molecular biological methods, and named Chlorella sp. BWY-1, The anaerobic digestion effluent used in this study was from a biogas plant with the raw materials of swine wastewater after solid-liquid separation. The Chlorella regularis (FACHB-729) was used as the control strain. The comparative study showed that Chlorella sp, BWY-Ihad relatively higher growth rate, biomass accumulation capacity and pollutants removal rate in BG11. and different concentrations of anaerobic digestion effluent. Chlorella sp. BWY-1 had the highest growth rate and biomass productivity (324.40 mg.L-1) in BG11, but its lipid productivity and lipid content increased with the increase of anaerobic digestion effluent concentration, In undiluted anaerobic digestion effluent, the lipid productivity and lipid content of Chlorella sp. BWY-1 were up to 44. 43% and 108. 70 mg.L-1, respectively. Those results showed that the isolated algal strain bad some potential applications in livestock wastewater treatment and bioenergy production, it could be combined with a solid-liquid separation, anaerobic fermentation and other techniques for processing livestock wastewater and producing biodiesel.

  17. Integrated synthesis of zeolites 4A and Na-P1 using coal fly ash for application in the formulation of detergents and swine wastewater treatment.

    PubMed

    Cardoso, Ariela M; Horn, Martha B; Ferret, Lizete S; Azevedo, Carla M N; Pires, Marçal

    2015-04-28

    Several researchers have reported zeolite synthesis using coal ash for a wide range of applications. However, little attention has been given to green processes, including moderate synthesis conditions, using waste as raw material and effluent reuse or reduction. In this study, Brazilian coal fly ashes were used for integrated synthesis of zeolites 4A and Na-P1 by two different routes and under moderate operating conditions (temperature and pressure). Both procedures produced zeolites with similar conversions (zeolite 4A at 82% purity and zeolite Na-P1 at 57-61%) and high CEC values (zeolites 4A: 4.5meqCa(2+)g(-1) and zeolites Na-P1: 2.6-2.8meqNH4(+)g(-1)). However, process 1 generated less effluent for the zeolite mass produced (7mLg(-1)), with low residual Si and Al levels and 74% of the Si available in the coal fly ash incorporated into the zeolite, while only 55% is used in process 2. For use as a builder in detergents, synthetic zeolite 4A exhibited conformity parameters equal to or greater than those of the commercial zeolite adopted as reference. Treatment of swine wastewater with zeolite Na-P1 resulted in a high removal capacity for total ammoniacal nitrogen (31mgg(-1)). Copyright © 2015 Elsevier B.V. All rights reserved.

  18. Effect of solids retention time on the bioavailability of organic carbon in anaerobically digested swine waste.

    PubMed

    Kinyua, Maureen N; Cunningham, Jeffrey; Ergas, Sarina J

    2014-06-01

    Anaerobic digestion (AD) can be used to stabilize and produce energy from livestock waste; however, digester effluents may require further treatment to remove nitrogen. This paper quantifies the effects of varying solids retention time (SRT) methane yield, volatile solids (VS) reduction and organic carbon bioavailability for denitrification during swine waste AD. Four bench-scale anaerobic digesters, with SRTs of 14, 21, 28 and 42 days, operated with swine waste feed. Effluent organic carbon bioavailability was measured using anoxic microcosms and respirometry. Excellent performance was observed for all four digesters, with >60% VS removal and CH4 yields between 0.1 and 0.3(m(3)CH4)/(kg VS added). Organic carbon in the centrate as an internal organic carbon source for denitrification supported maximum specific denitrification rates between 47 and 56(mg NO3(-)-N)/(g VSS h). The digester with the 21-day SRT had the highest CH4 yield and maximum specific denitrification rates. Copyright © 2014 Elsevier Ltd. All rights reserved.

  19. Transfer of oxytetracycline from swine manure to three different aquatic plants: implications for human exposure.

    PubMed

    Boonsaner, Maliwan; Hawker, Darryl W

    2015-03-01

    Little is known regarding the potential for pharmaceuticals including antibiotics to be accumulated in edible aquatic plants and enter the human food chain. This work investigates the transfer of a widely used veterinary antibiotic, oxytetracycline (OTC), from swine manure to aquatic plants by firstly characterizing desorption from swine manure to water and fitting data to both nonlinear and linear isotherms. Bioconcentration of OTC from water was then quantified with aquatic plants of contrasting morphology and growth habit viz. watermeal (Wolffia globosa Hartog and Plas), cabomba (Cabomba caroliniana A. Gray) and water spinach (Ipomoea aquatica Forsk.). Watermeal and water spinach are widely consumed in Southeast Asia. The OTC desorption and bioconcentration data were used to provide the first quantitative estimates of human exposure to OTC from a manure-water-aquatic plant route. Results show that under certain conditions (plants growing for 15d in undiluted swine manure effluent (2% w/v solids) and an initial OTC swine manure concentration of 43mgkg(-1) (dry weight)), this pathway could provide a significant fraction (>48%) of the acceptable daily intake (ADI) for OTC. While effluent dilution, lower OTC manure concentrations and not all plant material consumed being contaminated would be expected to diminish the proportion of the ADI accumulated, uptake from aquatic plants should not be ignored when determining human exposure to antibiotics such as OTC. Copyright © 2014 Elsevier Ltd. All rights reserved.

  20. Assessment of bacterial and archaeal community structure in Swine wastewater treatment processes.

    PubMed

    Da Silva, Marcio Luis Busi; Cantão, Mauricio Egídio; Mezzari, Melissa Paola; Ma, Jie; Nossa, Carlos Wolfgang

    2015-07-01

    Microbial communities from two field-scale swine wastewater treatment plants (WWTPs) were assessed by pyrosequencing analyses of bacterial and archaeal 16S ribosomal DNA (rDNA) fragments. Effluent samples from secondary (anaerobic covered lagoons and upflow anaerobic sludge blanket [UASB]) and tertiary treatment systems (open-pond natural attenuation lagoon and air-sparged nitrification-denitrification tank followed by alkaline phosphorus precipitation process) were analyzed. A total of 56,807 and 48,859 high-quality reads were obtained from bacterial and archaeal libraries, respectively. Dominant bacterial communities were associated with the phylum Firmicutes, Bacteroidetes, Proteobacteria, or Actinobacteria. Bacteria and archaea diversity were highest in UASB effluent sample. Escherichia, Lactobacillus, Bacteroides, and/or Prevotella were used as indicators of putative pathogen reduction throughout the WWTPs. Satisfactory pathogen reduction was observed after the open-pond natural attenuation lagoon but not after the air-sparged nitrification/denitrification followed by alkaline phosphorus precipitation treatment processes. Among the archaeal communities, 80% of the reads was related to hydrogeno-trophic methanogens Methanospirillum. Enrichment of hydrogenotrophic methanogens detected in effluent samples from the anaerobic covered lagoons and UASB suggested that CO2 reduction with H2 was the dominant methanogenic pathway in these systems. Overall, the results served to improve our current understanding of major microbial communities' changes downgradient from the pen and throughout swine WWTP as a result of different treatment processes.

  1. Comparison of constructed wetland and stabilization pond for the treatment of digested effluent of swine wastewater.

    PubMed

    Liu, Gang-Jin; Zheng, Dan; Deng, Liang-Wei; Wen, Quan; Liu, Yi

    2014-01-01

    A laboratory-scale horizontal subsurface flow constructed wetland (HSFCW) and a stabilization pond (SP) were constructed to compare their performances on the treatment of digested effluent of swine wastewater. After 457 days of operation, the removal efficiencies of the HSFCW were as follows: chemical oxygen demand (COD), 17-54%; total phosphorus (TP), 32-45% and ammonia nitrogen [Formula: see text], 27-88%, while they were 25-55%, 31-56% and 56-98%, respectively, for the SP, with a hydraulic retention time of 54 days and hydraulic loading of 0.01 m³ m⁻² d⁻¹. The average removed loads for the HSFCW were as follows: COD, 0.25-4.33; TP, 0.01-0.11 and [Formula: see text], 0.34-2.54 g m⁻² d⁻¹, while they were 0.25-4.45, 0.02-0.13 and 0.72-2.87 g m⁻² d⁻¹, respectively, for the SP. The SP performed better than the HSFCW because the SP showed a 20% of higher removal efficiency for [Formula: see text] than the HSFCW. Especially, the COD removal rate of SP was 10% higher than the HSFCW when the influent concentration was at the lowest and highest stages. Meanwhile, given the lower costs, the SP is more suitable for the treatment of digested effluent of swine wastewater than the HSFCW.

  2. Anaerobic digestion of olive oil mill effluents together with swine manure in UASB reactors.

    PubMed

    Aangelidaki, I; Ahrin, B K; Deng, H; Schmidt, J E

    2002-01-01

    Combined anaerobic digestion of olive oil mill effluent (OME) with swine manure, was investigated. In batch experiments was shown that for anaerobic degradation of OME alone nitrogen addition was needed. A COD:N ratio in the range of 65:1 to 126:1 was necessary for the optimal degradation process. Furthermore, it was found that methane productions rates during digestion of either swine manure alone or OME alone were much lower than the rates achieved when OME and manure were digested together. Admixing OME with manure at a concentration of 5 to 10% OME resulted in the highest methane production rates. Using upflow anaerobic sludge blanket (UASB) reactors, it was shown that codigestion of OME with swine manure (up to 50% OME) was successful with a COD reduction up to 75%. The process was adapted for degradation of OME with stepwise increase of the OME load to the UASB reactor. The results showed that the high content of ammonia in swine manure, together with content of other nutrients, make it possible to degrade OME without addition of external alkalinity and without addition of external nitrogen source. Anaerobic treatment of OME in UASB reactors resulted in reduction of simple phenolic compounds such as mequinol, phenyl ethyl alcohol and ethyl methyl phenol. After anaerobic treatment the concentration of these compounds was reduced between 75 and 100%. However, the concentration of some degradation products such as methyl phenol and ethyl phenol were detected in significantly higher concentrations after treatment, indicating that the process has to be further optimised to achieve satisfactory removal of all xenobiotic compounds.

  3. The effect of residual cationic polymers in swine wastewater on the fouling of reverse osmosis membranes.

    PubMed

    Pedersen, C O; Masse, L; Hjorth, M

    2014-01-01

    Solid-liquid separation with flocculation can be used as pre-treatment for reverse osmosis (RO) filtration as it produces a liquid fraction (LF) low in suspended solids (SS). However, residual polymers in the LF may foul the membrane. Membrane fouling during RO filtration of swine wastewater containing polymers was investigated with respect to polymer charge density (CD), effluent SS concentration and membrane surface charge. Effluents with 765 mg/L SS and without SS were spiked with low and medium CD polymers (0-40 mg/L effluent) then processed with RO membranes having low and high negative surface charges. Fouling intensity was evaluated by comparing permeate flux and water flux recovery of fouled and cleaned membranes. For effluents containing SS, the presence of polymer reduced permeate flux by 4-16% and water flux recovery of the fouled membrane by 0-18%, relative to effluents without polymer. The extent of the fouling was higher with the low than the medium CD polymer. The fouling was mostly reversible as cleaning allowed for over 95% flux recovery, but the membrane with high negative surface charge was more susceptible to irreversible fouling. Adding the low CD polymer to feed without SS had no effect on permeate flux or flux recovery. Membrane fouling thus appeared to be caused by the polymer changing SS-membrane interaction. If flocculation is applied to pre-treat manure, a medium CD polymer should be used to optimize SS removal and a membrane with low surface charge should be selected to minimize fouling.

  4. 40 CFR 412.47 - Additional measures.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 29 2014-07-01 2012-07-01 true Additional measures. 412.47 Section 412.47 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CONCENTRATED ANIMAL FEEDING OPERATIONS (CAFO) POINT SOURCE CATEGORY Swine, Poultry, and Veal...

  5. 40 CFR 412.47 - Additional measures.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 30 2012-07-01 2012-07-01 false Additional measures. 412.47 Section 412.47 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CONCENTRATED ANIMAL FEEDING OPERATIONS (CAFO) POINT SOURCE CATEGORY Swine, Poultry, and Veal...

  6. 40 CFR 412.47 - Additional measures.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 30 2013-07-01 2012-07-01 true Additional measures. 412.47 Section 412.47 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CONCENTRATED ANIMAL FEEDING OPERATIONS (CAFO) POINT SOURCE CATEGORY Swine, Poultry, and Veal...

  7. 40 CFR 412.47 - Additional measures.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Additional measures. 412.47 Section 412.47 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CONCENTRATED ANIMAL FEEDING OPERATIONS (CAFO) POINT SOURCE CATEGORY Swine, Poultry, and Veal...

  8. 40 CFR 412.41-412.42 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 30 2012-07-01 2012-07-01 false [Reserved] 412.41-412.42 Section 412.41-412.42 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CONCENTRATED ANIMAL FEEDING OPERATIONS (CAFO) POINT SOURCE CATEGORY Swine, Poultry, and...

  9. Isolation of an indigenous Chlorella vulgaris from swine wastewater and characterization of its nutrient removal ability in undiluted sewage.

    PubMed

    Wen, Yangmin; He, Yongjin; Ji, Xiaowei; Li, Shaofeng; Chen, Ling; Zhou, Youcai; Wang, Mingzi; Chen, Bilian

    2017-11-01

    Bio-treatment of wastewater mediated by microalgae is considered as a promising solution. This work aimed to isolate an indigenous microalgal strain (named MBFJNU-1) from swine wastewater effluent and identify as Chlorella vulgaris. After 12days, the removal efficiencies of total nitrogen (TN) and total phosphorus (TP) in undiluted swine slurry were 90.51% and 91.54%, respectively. Stress tolerance in response to wastewater was verified by cultivating in artificial wastewater containing different levels of chemical oxygen demand (COD), TN and TP. MBFJNU-1 could grow well in undiluted swine slurry and artificial wastewater containing 30,000mg/L COD or 2000mg/L TN. Furthermore, global nuclear DNA methylation (5-mC) of MBFJNU-1 was employed to explore the possible mechanism in response to wastewater stress. The results showed that the level of 5-mC was inversely proportional to the growth of MBFJNU-1 in different diluted swine slurry, helping to understand 5-mC variation in response to stress environment. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. 40 CFR 412.41-412.42 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 30 2013-07-01 2012-07-01 true [Reserved] 412.41-412.42 Section 412.41-412.42 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CONCENTRATED ANIMAL FEEDING OPERATIONS (CAFO) POINT SOURCE CATEGORY Swine, Poultry, and Veal...

  11. 40 CFR 412.41-412.42 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 29 2014-07-01 2012-07-01 true [Reserved] 412.41-412.42 Section 412.41-412.42 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CONCENTRATED ANIMAL FEEDING OPERATIONS (CAFO) POINT SOURCE CATEGORY Swine, Poultry, and Veal...

  12. 40 CFR 412.41-412.42 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 28 2010-07-01 2010-07-01 true [Reserved] 412.41-412.42 Section 412.41-412.42 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CONCENTRATED ANIMAL FEEDING OPERATIONS (CAFO) POINT SOURCE CATEGORY Swine, Poultry, and Veal...

  13. Separation of ammonia and phosphate minerals from wastewater using gas-permeable membranes

    USDA-ARS?s Scientific Manuscript database

    Conservation and recovery of nitrogen and phosphorus from animal wastes and municipal effluents is important because of economic and environmental reasons. In this paper we present a novel technology for separation and recovery of ammonia and phosphorus from liquid swine manure. Phosphorus recovery ...

  14. Nitrogen and Winter Cover Crop Effects on Spring and Summer Nutrient Uptake

    USDA-ARS?s Scientific Manuscript database

    Fertilization of bermudagrass [Cynodon dactylon (L.) Pers.] with swine-lagoon effluent in summer, April to September, does not match the period of productivity of the winter annual cover crops, annual ryegrass (Lolium multiflorum L.), cereal rye (Secale cereale), and berseem clover (Trifolium alexan...

  15. [Effect of pilot UASB-SFSBR-MAP process for the large scale swine wastewater treatment].

    PubMed

    Wang, Liang; Chen, Chong-Jun; Chen, Ying-Xu; Wu, Wei-Xiang

    2013-03-01

    In this paper, a treatment process consisted of UASB, step-fed sequencing batch reactor (SFSBR) and magnesium ammonium phosphate precipitation reactor (MAP) was built to treat the large scale swine wastewater, which aimed at overcoming drawbacks of conventional anaerobic-aerobic treatment process and SBR treatment process, such as the low denitrification efficiency, high operating costs and high nutrient losses and so on. Based on the treatment process, a pilot engineering was constructed. It was concluded from the experiment results that the removal efficiency of COD, NH4(+) -N and TP reached 95.1%, 92.7% and 88.8%, the recovery rate of NH4(+) -N and TP by MAP process reached 23.9% and 83.8%, the effluent quality was superior to the discharge standard of pollutants for livestock and poultry breeding (GB 18596-2001), mass concentration of COD, TN, NH4(+) -N, TP and SS were not higher than 135, 116, 43, 7.3 and 50 mg x L(-1) respectively. The process developed was reliable, kept self-balance of carbon source and alkalinity, reached high nutrient recovery efficiency. And the operating cost was equal to that of the traditional anaerobic-aerobic treatment process. So the treatment process could provide a high value of application and dissemination and be fit for the treatment pf the large scale swine wastewater in China.

  16. Recovery of ammonia and production of high-grade phosphates from digester effluents (municipal and livestock)

    USDA-ARS?s Scientific Manuscript database

    Phosphorus (P) recovery of anaerobically digested swine wastewater and side-stream municipal wastewater via magnesium precipitation was enhanced by combining it with the recovery of ammonia (NH3) through gas-permeable membranes and low-rate aeration. The low-rate aeration stripped the natural carbon...

  17. Antimicrobial-resistant bacterial populations and antimicrobial resistance genes obtained from environments impacted by livestock and municipal waste

    USDA-ARS?s Scientific Manuscript database

    This study compared the populations of antimicrobial-resistant bacteria and the repertoire of antimicrobial resistance genes in four environments: effluent of three municipal waste water treatment facilities, three cattle feedlot runoff catchment ponds, three swine waste lagoons, and two "low impact...

  18. Separation of swine wastewater into different concentration fractions and its contribution to combined anaerobic-aerobic process.

    PubMed

    Yang, Di; Deng, Liangwei; Zheng, Dan; Wang, Lan; Liu, Yi

    2016-03-01

    There are two problems associated with treatment of swine wastewater, low efficiency of anaerobic digestion during winter and poor performance for aerobic treatment of digested effluent. A strategy employing unbalanced distributions of the pollutant mass and wastewater volumes in anaerobic and aerobic units was proposed. To accomplish this, swine wastewater was separated into high content liquid (HCL) and low content liquid (LCL). Three separation ratios of HCL to LCL (v/v), 1:9 (S1), 2:8 (S2), and 3:7 (S3), were evaluated. Anaerobically digestion of the HCL accounted for only 10%, 20% and 30% of the total volume of raw wastewater, but produced 63.38%, 73.79% and 76.61% of the total methane output for S1, S2 and S3, respectively. The mixed liquid of digested effluents of HCL and LCL were treated aerobically using sequencing batch reactors. S2 generated the best performance, with removal efficiencies of 96.98% for COD, 98.95% for NH3-N, 91.69% for TN and 74.71% for TP. The results obtained for S1 were not as good as those for S2, but were better than those for S3. Based on methane output from the anaerobic unit and pollutants removal in the aerobic unit, S2 was the most suitable system for the treatment of swine wastewater. Additionally, the anaerobic digestion efficiency of S2 was 282% higher than that of previous techniques employing balanced distribution. Taken together, these findings indicate that unbalanced distribution could improve the efficiency of the anaerobic unit remarkably, while ensuring good performance of the aerobic unit. Copyright © 2015. Published by Elsevier Ltd.

  19. Digester effluent’s agronomic and odor emission potential: A swine case study

    USDA-ARS?s Scientific Manuscript database

    This on-farm study looked at the full-scale treatment effects of anaerobic digestion on the composition of manure effluent from an agronomic and air quality perspective. The goal was to improve our understanding of the role that anaerobic digestion may play in managing manure as a fertilizer and in...

  20. Enhancing recovery of ammonia from swine manure anaerobic digester effluent using gas-permeable membrane technology.

    PubMed

    Dube, P J; Vanotti, M B; Szogi, A A; García-González, M C

    2016-03-01

    Gas-permeable membrane technology is useful to recover ammonia from manure. In this study, the technology was enhanced using aeration instead of alkali chemicals to increase pH and the ammonium (NH4(+)) recovery rate. Digested effluents from covered anaerobic swine lagoons containing 1465-2097 mg NH4(+)-N L(-1) were treated using submerged membranes (0.13 cm(2) cm(-3)), low-rate aeration (120 mL air L-manure(-1) min(-1)) and nitrification inhibitor (22 mg L(-1)) to prevent nitrification. The experiment included a control without aeration. The pH of the manure with aeration rose from 8.6 to 9.2 while the manure without aeration decreased from 8.6 to 8.1. With aeration, 97-99% of the NH4(+) was removed in about 5 days of operation with 96-98% recovery efficiency. In contrast, without aeration it took 25 days to treat the NH4(+). Therefore, the recovery of NH4(+) was five times faster with the low-rate aeration treatment. This enhancement could reduce costs by 70%. Published by Elsevier Ltd.

  1. Growth optimisation of microalga mutant at high CO₂ concentration to purify undiluted anaerobic digestion effluent of swine manure.

    PubMed

    Cheng, Jun; Xu, Jiao; Huang, Yun; Li, Yuyou; Zhou, Junhu; Cen, Kefa

    2015-02-01

    Growth rate of the microalga Chlorella PY-ZU1 mutated by nuclear irradiation was optimised for use in the purification of undiluted anaerobic digestion effluent of swine manure (UADESM) with 3745 mg L(-1) chemical oxygen demand (COD) and 1135 mg L(-1) total nitrogen content. The problem of accessible carbon in UADESM was solved by continuous introduction of 15% (v/v) CO2. Adding phosphorus to UADESM and aeration of UADESM before inoculation both markedly reduced the lag phase of microalgal growth. In addition, the biomass yield and average growth rate of Chlorella PY-ZU1 increased significantly to 4.81 g L(-1) and 601.2 mg L(-1) d(-1), respectively, while the removal efficiencies of total phosphorus, COD and ammonia nitrogen increased to 95%, 79% and 73%, respectively. Thus, the findings indicate that Chlorella PY-ZU1 can be used for effective purification of UADESM, while the biomass can be safely used as animal feed supplement. Copyright © 2014 Elsevier Ltd. All rights reserved.

  2. Assessment of N2O emission from a photobioreactor treating ammonia-rich swine wastewater digestate.

    PubMed

    Mezzari, Melissa P; da Silva, Márcio L B; Nicoloso, Rodrigo S; Ibelli, Adriana M G; Bortoli, Marcelo; Viancelli, Aline; Soares, Hugo M

    2013-12-01

    This study investigated the interactions between naturally occurring bacteria and the microalgae Chlorella vulgaris within a lab scale photobioreactor treating ammonia-rich swine wastewater digestate effluent. Nitrification and denitrification were assessed by targeting ammonia monoxygenases (amoA), nitrate (narG), nitrite (nirS), nitric oxide (norB) and nitrous oxide (nosZ) reductases genes. Oxygen produced from microalgae photosynthesis stimulated nitrification. Under limiting carbon availability (i.e., <1.44 for mg TOC/mg NO2-N and 1.72 for mg TOC/mg NO3-N), incomplete denitrification led to accumulation of NO2 and NO3. Significant N2O emission (up to 118 μg N2O-N) was linked to NO2 metabolism in Chlorella. The addition of acetate as external carbon source recovered heterotrophic denitrification activity suppressing N2O emission. Effluent methane concentrations trapped within photobioreactor was removed concomitantly with ammonia. Overall, closed photobioreactors can be built to effectively remove nitrogen and mitigate simultaneously greenhouse gases emissions that would occur otherwise in open microalgae-based wastewater treatment systems. Copyright © 2013 Elsevier Ltd. All rights reserved.

  3. Nutrient recovery from swine waste and protein biomass production using duckweed ponds (Landoltia punctata): southern Brazil.

    PubMed

    Mohedano, R A; Velho, V F; Costa, R H R; Hofmann, S M; Belli Filho, P

    2012-01-01

    Brazil is one of the most important countries in pork production worldwide, ranking third. This activity has an important role in the national economic scenario. However, the fast growth of this activity has caused major environmental impacts, especially in developing countries. The large amount of nitrogen and phosphorus compounds found in pig manure has caused ecological imbalances, with eutrophication of major river basins in the producing regions. Moreover, much of the pig production in developing countries occurs on small farms, and therefore causes diffuse pollution. Therefore, duckweed pond have been successfully used in the swine waste polishing, generating further a biomass with high protein content. The present study evaluated the efficiency of two full scale duckweed ponds for the polishing of a small pig farm effluent, biomass yield and crude protein (CP) content. Duckweed pond series received the effluent from a biodigester-storage pond, with a flow rate of 1 m(3)/day (chemical oxygen demand rate = 186 kg/ha day) produced by 300 animals. After 1 year a great improvement of effluent quality was observed, with removal of 96% of total Kjeldahl nitrogen (TKN) and 89% of total phosphorus (TP), on average. Nitrogen removal rate is one of the highest ever found (4.4 g TKN/m(2) day). Also, the dissolved oxygen rose from 0.0 to 3.0 mg/L. The two ponds produced together over 13 tons of fresh biomass (90.5% moisture), with 35% of CP content, which represents a productivity of 24 tonsCP/ha year. Due to the high rate of nutrient removal, and also the high protein biomass production, duckweed ponds revealed, under the presented conditions, a great potential for the polishing and valorization of swine waste. Nevertheless, this technology should be better exploited to improve the sustainability of small pig farms in order to minimize the impacts of this activity on the environment.

  4. Bioprocessing for elimination antibiotics and hormones from swine wastewater.

    PubMed

    Cheng, D L; Ngo, H H; Guo, W S; Liu, Y W; Zhou, J L; Chang, S W; Nguyen, D D; Bui, X T; Zhang, X B

    2018-04-15

    Antibiotics and hormones in swine wastewater have become a critical concern worldwide due to the severe threats to human health and the eco-environment. Removal of most detectable antibiotics and hormones, such as sulfonamides (SAs), SMs, tetracyclines (TCs), macrolides, and estrogenic hormones from swine wastewater utilizing various biological processes were summarized and compared. In biological processes, biosorption and biodegradation are the two major removal mechanisms for antibiotics and hormones. The residuals in treated effluents and sludge of conventional activated sludge and anaerobic digestion processes can still pose risks to the surrounding environment, and the anaerobic processes' removal efficiencies were inferior to those of aerobic processes. In contrast, membrane bioreactors (MBRs), constructed wetlands (CWs) and modified processes performed better because of their higher biodegradation of toxicants. Process modification on activated sludge, anaerobic digestion and conventional MBRs could also enhance the performance (e.g. removing up to 98% SMs, 88.9% TCs, and 99.6% hormones from wastewater). The hybrid process combining MBRs with biological or physical technology also led to better removal efficiency. As such, modified conventional biological processes, advanced biological technologies and MBR hybrid systems are considered as a promising technology for removing toxicants from swine wastewater. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Nitrogen recovery from wastewater using gas-permeable membranes: Impact of inorganic carbon content and natural organic matter.

    PubMed

    Daguerre-Martini, S; Vanotti, M B; Rodriguez-Pastor, M; Rosal, A; Moral, R

    2018-06-15

    Gas-permeable membranes coupled with low-rate aeration is useful to recover ammonia (NH 4 + ) from livestock effluents. In this study, the role of inorganic carbon (bicarbonate, HCO 3 - ) to enhance the N recovery process was evaluated using synthetic effluents with various NH 4 + to HCO 3 - molar ratios of 0.5, 1.0, 1.5 and 2.0. The study also evaluated the effect of increased organic matter on the NH 4 + recovery using humic acids (3000-6000 mg L -1 ), and the N recovery from high-strength swine manure. The release of hydroxide from the HCO 3 - with aeration increased the wastewater pH and promoted gaseous ammonia formation and membrane uptake. At the same time, the recovery of gaseous ammonia (NH 3 ) through the membrane acidified the wastewater. Therefore, an abundant inorganic carbon supply in balance with the NH 4 + is needed for a successful operation of the technology. NH 4 + removal efficiencies >96% were obtained with NH 4 + to HCO 3 - ratios ≤1. However, higher molar ratios inhibited the N recovery process resulting in lower efficiencies (<65%). Fortunately, most swine manures contain ample supply of endogenous inorganic carbon and the process can be used to more economically recover the ammonia using the natural inorganic carbon instead of expensive alkali chemicals. In 4 days, the recovered NH 4 + from swine manure contained 48,000 mg L -1 . Finally, it was found the process was not inhibited by the increasing levels of organic matter in the wastewater evaluated. Published by Elsevier Ltd.

  6. Runoff nutrient transport as affected by land application method, swine growth stage, and runoff rate

    USDA-ARS?s Scientific Manuscript database

    This study was conducted to measure the effects of slurry application method, swine growth stage, and flow rate on runoff nutrient transport. Swine slurry was obtained from production units containing grower pigs, finisher pigs, or sows and gilts. The swine slurry was applied using broadcast, disk, ...

  7. 9 CFR 93.504 - Import permits for swine and for swine specimens for diagnostic purposes; and reservation fees...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... maintained by APHIS. (a) Application for permit; reservation required. (1) For swine and swine test specimens..., number or quantity of swine or swine test specimens to be imported; the purpose of the importation... port of entry in the United States; the proposed date of arrival of the swine or swine test specimens...

  8. 9 CFR 93.504 - Import permits for swine and for swine specimens for diagnostic purposes; and reservation fees...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... maintained by APHIS. (a) Application for permit; reservation required. (1) For swine and swine test specimens..., number or quantity of swine or swine test specimens to be imported; the purpose of the importation... port of entry in the United States; the proposed date of arrival of the swine or swine test specimens...

  9. 9 CFR 93.504 - Import permits for swine and for swine specimens for diagnostic purposes; and reservation fees...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... maintained by APHIS. (a) Application for permit; reservation required. (1) For swine and swine test specimens..., number or quantity of swine or swine test specimens to be imported; the purpose of the importation... port of entry in the United States; the proposed date of arrival of the swine or swine test specimens...

  10. Dual permeability modeling of tile drain management influences on hydrologic and nutrient transport characteristics in macroporous soil

    NASA Astrophysics Data System (ADS)

    Frey, Steven K.; Hwang, Hyoun-Tae; Park, Young-Jin; Hussain, Syed I.; Gottschall, Natalie; Edwards, Mark; Lapen, David R.

    2016-04-01

    Tile drainage management is considered a beneficial management practice (BMP) for reducing nutrient loads in surface water. In this study, 2-dimensional dual permeability models were developed to simulate flow and transport following liquid swine manure and rhodamine WT (strongly sorbing) tracer application on macroporous clay loam soils under controlled (CD) and free drainage (FD) tile management. Dominant flow and transport characteristics were successfully replicated, including higher and more continuous tile discharge and lower peak rhodamine WT concentrations in FD tile effluent; in relation to CD, where discharge was intermittent, peak rhodamine concentrations higher, and mass exchange from macropores into the soil matrix greater. Explicit representation of preferential flow was essential, as macropores transmitted >98% of surface infiltration, tile flow, and tile solute loads for both FD and CD. Incorporating an active 3rd type lower boundary condition that facilitated groundwater interaction was imperative for simulating CD, as the higher (relative to FD) water table enhanced water and soluble nutrient movement from the soil profile into deeper groundwater. Scenario analysis revealed that in conditions where slight upwards hydraulic gradients exist beneath tiles, groundwater upwelling can influence the concentration of surface derived solutes in tile effluent under FD conditions; whereas the higher and flatter CD water table can restrict groundwater upwelling. Results show that while CD can reduce tile discharge, it can also lead to an increase in surface-application derived nutrient concentrations in tile effluent and hence surface water receptors, and it can promote NO3 loading into groundwater. This study demonstrates dual permeability modeling as a tool for increasing the conceptual understanding of tile drainage BMPs.

  11. Comprehensive evaluation of nitrogen removal rate and biomass, ethanol, and methane production yields by combination of four major duckweeds and three types of wastewater effluent.

    PubMed

    Toyama, Tadashi; Hanaoka, Tsubasa; Tanaka, Yasuhiro; Morikawa, Masaaki; Mori, Kazuhiro

    2018-02-01

    To assess the potential of duckweeds as agents for nitrogen removal and biofuel feedstocks, Spirodela polyrhiza, Lemna minor, Lemna gibba, and Landoltia punctata were cultured in effluents of municipal wastewater, swine wastewater, or anaerobic digestion for 4 days. Total dissolved inorganic nitrogen (T-DIN) of 20-50 mg/L in effluents was effectively removed by inoculating with 0.3-1.0 g/L duckweeds. S. polyrhiza showed the highest nitrogen removal (2.0-10.8 mg T-DIN/L/day) and biomass production (52.6-70.3 mg d.w./L/day) rates in all the three effluents. Ethanol and methane were produced from duckweed biomass grown in each effluent. S. polyrhiza and L. punctata biomass showed higher ethanol (0.168-0.191, 0.166-0.172 and 0.174-0.191 g-ethanol/g-biomass, respectively) and methane (340-413 and 343-408 NL CH 4 /kg VS, respectively) production potentials than the others, which is related to their higher carbon and starch contents and calorific values. Copyright © 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.

  12. The stability of accumulating nitrite from Swine wastewater in a sequencing batch reactor.

    PubMed

    Wang, Liang; Zhu, Jun; Miller, Curtis

    2011-02-01

    Shortcut nitrification is the first step of shortcut nitrogen removal from swine wastewater. Stably obtaining an effluent with a significant amount of nitrite is the premise for the subsequent shortcut denitrification. In this paper, the stability of nitrite accumulation was investigated using a 1.5-day hydraulic retention time in a 10-L (working volume) activated sludge sequencing batch reactor (SBR) with an 8-h cycle consisted of 4 h 38 min aerobic feeding, 1 h 22 min aerobic reaction, 30 min settling, 24 min withdrawal, and 1 h 6 min idle. The nitrite production stability was tested using four different ammonium loading rates, 0.075, 0.062, 0.053, and 0.039 g NH(4)-N/g (mixed liquid suspended solid, MLSS) day in a 2-month running period. The total inorganic nitrogen composition in the effluent was not affected when the ammonium load was between 0.053 and 0.075 g NH(4)-N/g MLSS · day (64% NO(2)-N, 16% NO(3)-N, and 20% NH(4)-N). Under 0.039 g NH(4)-N/g MLSS · day, more NO(2)-N was transformed to NO(3)-N with an effluent of 60% NO(2)-N, 20% NO(3)-N, and 20% NH(4)-N. The reducing load test was able to show the relationship between a declining free nitrous acid (FNA) concentration and the decreasing nitrite production, indicating that the inhibition of FNA on nitrite oxidizing bacteria depends on its levels and an ammonium loading rate around 0.035 g NH(4)-N/g MLSS · day is the lower threshold for producing a nitrite dominance effluent in the activated sludge SBR under the current settings.

  13. In Vitro Continuous Fermentation Model (PolyFermS) of the Swine Proximal Colon for Simultaneous Testing on the Same Gut Microbiota

    PubMed Central

    Tanner, Sabine A.; Zihler Berner, Annina; Rigozzi, Eugenia; Grattepanche, Franck; Chassard, Christophe; Lacroix, Christophe

    2014-01-01

    In vitro gut modeling provides a useful platform for a fast and reproducible assessment of treatment-related changes. Currently, pig intestinal fermentation models are mainly batch models with important inherent limitations. In this study we developed a novel in vitro continuous fermentation model, mimicking the porcine proximal colon, which we validated during 54 days of fermentation. This model, based on our recent PolyFermS design, allows comparing different treatment effects on the same microbiota. It is composed of a first-stage inoculum reactor seeded with immobilized fecal swine microbiota and used to constantly inoculate (10% v/v) five second-stage reactors, with all reactors fed with fresh nutritive chyme medium and set to mimic the swine proximal colon. Reactor effluents were analyzed for metabolite concentrations and bacterial composition by HPLC and quantitative PCR, and microbial diversity was assessed by 454 pyrosequencing. The novel PolyFermS featured stable microbial composition, diversity and metabolite production, consistent with bacterial activity reported for swine proximal colon in vivo. The constant inoculation provided by the inoculum reactor generated reproducible microbial ecosystems in all second-stage reactors, allowing the simultaneous investigation and direct comparison of different treatments on the same porcine gut microbiota. Our data demonstrate the unique features of this novel PolyFermS design for the swine proximal colon. The model provides a tool for efficient, reproducible and cost-effective screening of environmental factors, such as dietary additives, on pig colonic fermentation. PMID:24709947

  14. In vitro continuous fermentation model (PolyFermS) of the swine proximal colon for simultaneous testing on the same gut microbiota.

    PubMed

    Tanner, Sabine A; Zihler Berner, Annina; Rigozzi, Eugenia; Grattepanche, Franck; Chassard, Christophe; Lacroix, Christophe

    2014-01-01

    In vitro gut modeling provides a useful platform for a fast and reproducible assessment of treatment-related changes. Currently, pig intestinal fermentation models are mainly batch models with important inherent limitations. In this study we developed a novel in vitro continuous fermentation model, mimicking the porcine proximal colon, which we validated during 54 days of fermentation. This model, based on our recent PolyFermS design, allows comparing different treatment effects on the same microbiota. It is composed of a first-stage inoculum reactor seeded with immobilized fecal swine microbiota and used to constantly inoculate (10% v/v) five second-stage reactors, with all reactors fed with fresh nutritive chyme medium and set to mimic the swine proximal colon. Reactor effluents were analyzed for metabolite concentrations and bacterial composition by HPLC and quantitative PCR, and microbial diversity was assessed by 454 pyrosequencing. The novel PolyFermS featured stable microbial composition, diversity and metabolite production, consistent with bacterial activity reported for swine proximal colon in vivo. The constant inoculation provided by the inoculum reactor generated reproducible microbial ecosystems in all second-stage reactors, allowing the simultaneous investigation and direct comparison of different treatments on the same porcine gut microbiota. Our data demonstrate the unique features of this novel PolyFermS design for the swine proximal colon. The model provides a tool for efficient, reproducible and cost-effective screening of environmental factors, such as dietary additives, on pig colonic fermentation.

  15. Soil microbial properties after long-term swine slurry application to conventional and no-tillage systems in Brazil.

    PubMed

    Balota, Elcio L; Machineski, Oswaldo; Hamid, Karima I A; Yada, Ines F U; Barbosa, Graziela M C; Nakatani, Andre S; Coyne, Mark S

    2014-08-15

    Swine waste can be used as an agricultural fertilizer, but large amounts may accumulate excess nutrients in soil or contaminate the surrounding environment. This study evaluated long-term soil amendment (15 years) with different levels of swine slurry to conventional (plow) tillage (CT) and no tillage (NT) soils. Long-term swine slurry application did not affect soil organic carbon. Some chemical properties, such as calcium, base saturation, and aluminum saturation were significantly different within and between tillages for various application rates. Available P and microbial parameters were significantly affected by slurry addition. Depending on tillage, soil microbial biomass and enzyme activity increased up to 120 m(3) ha(-1) year(-1) in all application rates. The NT system had higher microbial biomass and activity than CT at all application levels. There was an inverse relationship between the metabolic quotient (qCO2) and MBC, and the qCO2 was 53% lower in NT than CT. Swine slurry increased overall acid phosphatase activity, but the phosphatase produced per unit of microbial biomass decreased. A comparison of data obtained in the 3rd and 15th years of swine slurry application indicated that despite slurry application the CT system degraded with time while the NT system had improved values of soil quality indicators. For these Brazilian oxisols, swine slurry amendment was insufficient to maintain soil quality parameters in annual crop production without additional changes in tillage management. Copyright © 2014 Elsevier B.V. All rights reserved.

  16. Fate and transport of antibiotic resistant bacteria and resistance genes in artificially drained agricultural fields receiving swine manure application

    USDA-ARS?s Scientific Manuscript database

    While previous studies have examined the occurrence of antibiotic resistant bacteria and antibiotic resistant genes around confined swine feeding operations, little information is known about their release and transport from artificially drained fields receiving swine manure application. Much of the...

  17. Effect of Solids Retention Time on the Denitrification Potential of Anaerobically Digested Swine Waste

    NASA Astrophysics Data System (ADS)

    Kinyua, Maureen Njoki

    Three continuously stirred tank reactors (CSTR) were operated in semi continuous mode treating swine waste using anaerobic digestion. The reactors were used to test the effect of solid retention time (SRT) on CH4 yield, total ammonia nitrogen (TAN) concentrations, % volatile solids (VS), chemical oxygen demand (COD) and volatile fatty acids (VFA) removal, readily biodegradable COD concentration and the denitrification potential for the effluent in a biological nutrient removal (BNR) system. During Phase I of the study, the three reactors were operated at the same 28 day SRT for 16 weeks. SRTs were then changed during the 12 week Phase II period. The SRTs studied were 14, 21 and 28 days, with the same organic loading rate (OLR) of 1.88 ± 0.2 kg VS/ m3-day. The reactor with the lowest SRT (14 days) had the highest VS and VFA removal at 73.6 and 67.6% and lowest TAN concentration at 0.78 g NH4+-N/L, followed by the 21 day and 28 day reactors. This was likely due to the fast microbial growth rates and substrate utilization rates in this reactor compared with the other two. The 14 day reactor had the highest CH4 yield at 0.33 m3CH 4/kg VS added and readily biodegradable COD concentration at 0.93 COD/L. The variations in CH4 yield and readily biodegradable COD concentrations between the three reactors were not statistically significant. Denitrification potential for the reactors was 1.20, 0.73 and 0.56 g COD/g N for 14, 21 and 28 day reactors, respectively, and the differences were statistically significant. None of the reactors achieved a denitrification potential of 5 g COD/g N, the amount required to use effluent of anaerobically digested swine waste as an internal carbon source in a BNR. This was attributed to operating conditions such as freezing and thawing of the raw swine waste that maximized CH4 yield and lowered the readily biodegradable COD concentration. In addition the 14 day reactor had low TAN concentrations thus increasing the denitrification potential of the centrate from that reactor.

  18. Potential Sources and Transmission of Salmonella and Antimicrobial Resistance in Kampala, Uganda

    PubMed Central

    Afema, Josephine A.; Byarugaba, Denis K.; Shah, Devendra H.; Atukwase, Esther; Nambi, Maria; Sischo, William M.

    2016-01-01

    In sub‒Saharan Africa, non‒typhoidal Salmonellae (NTS) cause invasive disease particularly in children and HIV infected adults, but the disease epidemiology is poorly understood. Between 2012 and 2013, we investigated NTS sources and transmission in Kampala. We detected Salmonella in 60% of the influent and 60% of the effluent samples from a wastewater treatment plant and 53.3% of the influent and 10% of the effluent samples from waste stabilization ponds that serve the human population; 40.9% of flush‒water samples from ruminant slaughterhouses, 6.6% of the poultry fecal samples from live bird markets and 4% of the fecal samples from swine at slaughter; and in 54.2% of the water samples from a channel that drains storm–water and effluents from the city. We obtained 775 Salmonella isolates, identified 32 serovars, and determined resistance to 15 antimicrobials. We genotyped common serovars using multiple‒locus variable number tandem repeats analysis or pulsed‒field gel electrophoresis. In addition, we analyzed 49 archived NTS isolates from asymptomatic livestock and human clinical cases. Salmonella from ruminant and swine sources were mostly pan‒susceptible (95%) while poultry isolates were generally more resistant. Salmonella Kentucky isolated from poultry exhibited extensive drug resistance characterized by resistance to 10 antimicrobials. Interestingly, similar genotypes of S. Kentucky but with less antimicrobial resistance (AMR) were found in poultry, human and environmental sources. The observed AMR patterns could be attributed to host or management factors associated with production. Alternatively, S. Kentucky may be prone to acquiring AMR. The factors driving AMR remain poorly understood and should be elucidated. Overall, shared genotypes and AMR phenotypes were found in NTS from human, livestock and environmental sources, suggesting zoonotic and environmental transmissions most likely occur. Information from this study could be used to control NTS transmission. PMID:26999788

  19. Upflow anaerobic sludge blanket and aerated constructed wetlands for swine wastewater treatment: a pilot study.

    PubMed

    Masi, F; Rizzo, A; Martinuzzi, N; Wallace, S D; Van Oirschot, D; Salazzari, P; Meers, E; Bresciani, R

    2017-07-01

    Swine wastewater management is often affected by two main issues: a too high volume for optimal reuse as a fertilizer and a too high strength for an economically sustainable treatment by classical solutions. Hence, an innovative scheme has been tested to treat swine wastewater, combining a low cost anaerobic reactor, upflow anaerobic sludge blanket (UASB), with intensified constructed wetlands (aerated CWs) in a pilot scale experimental study. The swine wastewater described in this paper is produced by a swine production facility situated in North Italy. The scheme of the pilot plant consisted of: (i) canvas-based thickener; (ii) UASB; (iii) two intensified aerated vertical subsurface flow CWs in series; (iv) a horizontal flow subsurface CW. The influent wastewater quality has been defined for total suspended solids (TSS 25,025 ± 9,323 mg/l), organic carbon (chemical oxygen demand (COD) 29,350 ± 16,983 mg/l), total reduced nitrogen and ammonium (total Kjeldahl nitrogen (TKN) 1,783 ± 498 mg/l and N-NH 4 + 735 ± 251 mg/l) and total phosphorus (1,285 ± 270 mg/l), with nitrates almost absent. The overall system has shown excellent performances in terms of TSS, COD, N-NH 4 + and TKN removal efficiencies (99.9%, 99.6%, 99.5%, and 99.0%, respectively). Denitrification (N-NO 3 - effluent concentration equal to 614 ± 268 mg/l) did not meet the Italian quality standards for discharging in water bodies, mainly because the organic carbon was almost completely removed in the intensified CW beds.

  20. DEVELOPMENT OF AN EMISSION FACTOR FOR AMMONIA EMISSIONS FROM U.S. SWINE FARMS BASED ON FIELD TESTS AND APPLICATION OF A MASS BALANCE METHOD

    EPA Science Inventory

    This paper summarizes and discusses recent available U.S. and European information on
    ammonia (NH3) emissions from swine farms and assesses the applicability for general use
    in the United States. The emission rates for the swine barns calculated by various methods show
    g...

  1. Assessment of swine-specific bacteriophages of Bacteroides fragilis in swine farms with different antibiotic practices.

    PubMed

    Leknoi, Yuranan; Mongkolsuk, Skorn; Sirikanchana, Kwanrawee

    2017-04-01

    We assessed the occurrence and specificity of bacteriophages of Bacteroides fragilis in swine farms for their potential application in microbial source tracking. A local B. fragilis host strain, SP25 (DSM29413), was isolated from a pooled swine feces sample taken from a non-antibiotic farm. This strain was highly specific to swine fecal materials because it did not detect bacteriophages in any samples from human sewage, sheep, goats, cattle, dogs, and cats. The reference B. fragilis strain, RYC2056, could detect phages in swine samples but also detected phages in most human sewage and polluted urban canal samples. Phages of SP25 exist in the proximity of certain swine farms, regardless of their antibiotic use (p > 0.05). B. fragilis strain SP25 exhibited relatively high resistance to most of the veterinary antimicrobial agents tested. Interestingly, most farms that were positive for SP25 phages were also positive for RYC2056 phages. In conclusion, the swine-specific SP25 strain has the potential to indicate swine fecal contamination in certain bodies of water. Bacterial isolates with larger distributions are being studied and validated. This study highlights the importance of assessing the abundance of phages in local swine populations before determining their potential applicability for source tracking in local surface waters.

  2. Advanced treatment technique for swine wastewater using two agents: Thermally polymerized amorphous silica and hydrated lime for color and phosphorus removal and sulfur for nitrogen removal.

    PubMed

    Hasegawa, Teruaki; Kurose, Yohei; Tanaka, Yasuo

    2017-10-01

    The efficacy of advanced treatment of swine wastewater using thermally polymerized, modified amorphous silica and hydrated lime (M-CSH-lime) for color and phosphorus removal and sulfur for nitrogen removal was examined with a demonstration-scale treatment plant. The color removal rate was approximately 78% at M-CSH-lime addition rates of > 0.055 wt/v%. The PO43--P removal rate exceeded 99.9% with > 0.023 wt/v%. pH of the effluent from the M-CSH-lime reactor increased with the addition rate till a maximum value of 12.7, which was effective in disinfection. The recovered M-CSH-lime would be suitable as a phosphorus fertilizer because the total P 2 O 5 content was approximately 10%. The nitrogen oxide (NOx-N) removal rate by sulfur denitrification increased to approximately 80% when the NOx-N loading rate was around 0.1 kg-N/ton-S/day. It was suggested that the combination of the two processes would be effective in the advanced treatment of swine wastewater. © 2017 Japanese Society of Animal Science.

  3. 9 CFR 167.1 - Scope and applicability of rules of practice.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., DEPARTMENT OF AGRICULTURE SWINE HEALTH PROTECTION RULES OF PRACTICE GOVERNING PROCEEDINGS UNDER THE SWINE... under sections 5 and 6 of the Swine Health Protection Act (7 U.S.C. 3804, 3805). In addition, the...

  4. 9 CFR 167.1 - Scope and applicability of rules of practice.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., DEPARTMENT OF AGRICULTURE SWINE HEALTH PROTECTION RULES OF PRACTICE GOVERNING PROCEEDINGS UNDER THE SWINE... under sections 5 and 6 of the Swine Health Protection Act (7 U.S.C. 3804, 3805). In addition, the...

  5. 9 CFR 167.1 - Scope and applicability of rules of practice.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., DEPARTMENT OF AGRICULTURE SWINE HEALTH PROTECTION RULES OF PRACTICE GOVERNING PROCEEDINGS UNDER THE SWINE... under sections 5 and 6 of the Swine Health Protection Act (7 U.S.C. 3804, 3805). In addition, the...

  6. 9 CFR 167.1 - Scope and applicability of rules of practice.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., DEPARTMENT OF AGRICULTURE SWINE HEALTH PROTECTION RULES OF PRACTICE GOVERNING PROCEEDINGS UNDER THE SWINE... under sections 5 and 6 of the Swine Health Protection Act (7 U.S.C. 3804, 3805). In addition, the...

  7. 9 CFR 167.1 - Scope and applicability of rules of practice.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., DEPARTMENT OF AGRICULTURE SWINE HEALTH PROTECTION RULES OF PRACTICE GOVERNING PROCEEDINGS UNDER THE SWINE... under sections 5 and 6 of the Swine Health Protection Act (7 U.S.C. 3804, 3805). In addition, the...

  8. Hepatitis E Virus (Genotype 3) in Slurry Samples from Swine Farming Activities in Italy.

    PubMed

    La Rosa, G; Della Libera, S; Brambilla, M; Bisaglia, C; Pisani, G; Ciccaglione, A R; Bruni, R; Taffon, S; Equestre, M; Iaconelli, M

    2017-06-01

    Hepatitis E virus (HEV) is an emergent causative agent of acute hepatitis, transmitted by fecal-oral route. Infection with HEV is a global cause for morbidity and mortality throughout the world: it mainly causes large outbreaks in endemic areas and sporadic autochthonous cases in industrialized countries where HEV infections seem to be an emergent zoonotic disease. Infection of porcine livestock and its relationship with the human cases have been demonstrated. The present study describes an investigation on the prevalence and diversity of HEV in pig slurry in Italy. Slurry samples (24) were collected from ten farms located in North Italy during 2015 and analyzed for HEV, using four broad-range nested PCR assays targeting ORF1 (MTase), ORF2 (capsid) genes, and ORF2/3 regions. Overall, 18 samples (75%) were positive for HEV RNA, and characterized as genotype 3. Nine samples could be subtyped by ORF2 sequencing: Eight belonged to subtype 3f, while one sequence could not be characterized by blast analysis and phylogenetic analysis and may actually represent a new subtype. Furthermore, similarity of 99% was found between 3f Italian HEV sequences of human and swine origins. Real-Time PCR assay was also performed, in order to obtain quantitative data on positive samples. Two swine slurry samples were positive, containing 600 and 1000 UI per mL of sewage. The results of this study show that HEV strains belonging to zoonotic genotype 3 are widely present in swine excreta, and have high degree of identity with strains detected in autochthonous HEV cases. Improving swine farming operations safety and increasing operators' awareness of the zoonotic potential connected with the handling of swine effluents turn out to be key points in order to reduce the environmental and sanitary problem represented by the possible dissemination of HEV to water bodies.

  9. Antimicrobial-Resistant Bacterial Populations and Antimicrobial Resistance Genes Obtained from Environments Impacted by Livestock and Municipal Waste

    PubMed Central

    Durso, Lisa M.; Harhay, Dayna M.; Schmidt, John W.

    2015-01-01

    This study compared the populations of antimicrobial-resistant bacteria and the repertoire of antimicrobial resistance genes in four environments: effluent of three municipal wastewater treatment facilities, three cattle feedlot runoff catchment ponds, three swine waste lagoons, and two “low impact” environments (an urban lake and a relict prairie). Multiple liquid and solid samples were collected from each environment. The prevalences and concentrations of antimicrobial-resistant (AMR) Gram-negative (Escherichia coli and Salmonella enterica) and Gram-positive (enterococci) bacteria were determined from individual samples (n = 174). The prevalences of 84 antimicrobial resistance genes in metagenomic DNA isolated from samples pooled (n = 44) by collection date, location, and sample type were determined. The prevalences and concentrations of AMR E. coli and Salmonella were similar among the livestock and municipal sample sources. The levels of erythromycin-resistant enterococci were significantly higher in liquid samples from cattle catchment ponds and swine waste lagoons than in liquid samples from municipal wastewater treatment facilities, but solid samples from these environments did not differ significantly. Similarly, trimethoprim/sulfamethoxazole-resistant E. coli concentrations were significantly higher in swine liquid than in municipal liquid samples, but there was no difference in solid samples. Multivariate analysis of the distribution of antimicrobial resistance genes using principal coordinate analysis showed distinct clustering of samples with livestock (cattle and swine), low impact environment and municipal samples forming three separate clusters. The numbers of class A beta-lactamase, class C beta-lactamase, and fluoroquinolone resistance genes detected were significantly higher (P < 0.05) in municipal samples than in cattle runoff or swine lagoon samples. In conclusion, we report that AMR is a very widespread phenomenon and that similar prevalences and concentrations of antimicrobial-resistant bacteria and antimicrobial resistance genes exist in cattle, human, and swine waste streams, but a higher diversity of antimicrobial resistance genes are present in treated human waste discharged from municipal wastewater treatment plants than in livestock environments. PMID:26197056

  10. Antimicrobial-Resistant Bacterial Populations and Antimicrobial Resistance Genes Obtained from Environments Impacted by Livestock and Municipal Waste.

    PubMed

    Agga, Getahun E; Arthur, Terrance M; Durso, Lisa M; Harhay, Dayna M; Schmidt, John W

    2015-01-01

    This study compared the populations of antimicrobial-resistant bacteria and the repertoire of antimicrobial resistance genes in four environments: effluent of three municipal wastewater treatment facilities, three cattle feedlot runoff catchment ponds, three swine waste lagoons, and two "low impact" environments (an urban lake and a relict prairie). Multiple liquid and solid samples were collected from each environment. The prevalences and concentrations of antimicrobial-resistant (AMR) Gram-negative (Escherichia coli and Salmonella enterica) and Gram-positive (enterococci) bacteria were determined from individual samples (n = 174). The prevalences of 84 antimicrobial resistance genes in metagenomic DNA isolated from samples pooled (n = 44) by collection date, location, and sample type were determined. The prevalences and concentrations of AMR E. coli and Salmonella were similar among the livestock and municipal sample sources. The levels of erythromycin-resistant enterococci were significantly higher in liquid samples from cattle catchment ponds and swine waste lagoons than in liquid samples from municipal wastewater treatment facilities, but solid samples from these environments did not differ significantly. Similarly, trimethoprim/sulfamethoxazole-resistant E. coli concentrations were significantly higher in swine liquid than in municipal liquid samples, but there was no difference in solid samples. Multivariate analysis of the distribution of antimicrobial resistance genes using principal coordinate analysis showed distinct clustering of samples with livestock (cattle and swine), low impact environment and municipal samples forming three separate clusters. The numbers of class A beta-lactamase, class C beta-lactamase, and fluoroquinolone resistance genes detected were significantly higher (P < 0.05) in municipal samples than in cattle runoff or swine lagoon samples. In conclusion, we report that AMR is a very widespread phenomenon and that similar prevalences and concentrations of antimicrobial-resistant bacteria and antimicrobial resistance genes exist in cattle, human, and swine waste streams, but a higher diversity of antimicrobial resistance genes are present in treated human waste discharged from municipal wastewater treatment plants than in livestock environments.

  11. 9 CFR 98.38 - Restrictions on the importation of swine semen from the APHIS-defined European CSF region.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... swine semen from the APHIS-defined European CSF region. 98.38 Section 98.38 Animals and Animal Products... Certain Animal Semen § 98.38 Restrictions on the importation of swine semen from the APHIS-defined European CSF region. In addition to meeting all other applicable provisions of this part, swine semen...

  12. 9 CFR 98.38 - Restrictions on the importation of swine semen from the APHIS-defined European CSF region.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... swine semen from the APHIS-defined European CSF region. 98.38 Section 98.38 Animals and Animal Products... Certain Animal Semen § 98.38 Restrictions on the importation of swine semen from the APHIS-defined European CSF region. In addition to meeting all other applicable provisions of this part, swine semen...

  13. 9 CFR 98.38 - Restrictions on the importation of swine semen from the APHIS-defined European CSF region.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... swine semen from the APHIS-defined European CSF region. 98.38 Section 98.38 Animals and Animal Products... Certain Animal Semen § 98.38 Restrictions on the importation of swine semen from the APHIS-defined European CSF region. In addition to meeting all other applicable provisions of this part, swine semen...

  14. 40 CFR 430.114 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... effluent reduction attainable by the application of the best available technology economically achievable... representing the degree of effluent reduction attainable by the application of the best available technology...

  15. 40 CFR 440.102 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... degree of effluent reduction attainable by the application of the best practicable control technology... degree of effluent reduction attainable by the application of the best practicable control technology...

  16. 40 CFR 440.82 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... effluent reduction attainable by the application of the best practicable control technology currently... degree of effluent reduction attainable by the application of the best practicable control technology...

  17. 40 CFR 440.82 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... effluent reduction attainable by the application of the best practicable control technology currently... degree of effluent reduction attainable by the application of the best practicable control technology...

  18. 40 CFR 430.114 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... effluent reduction attainable by the application of the best available technology economically achievable... representing the degree of effluent reduction attainable by the application of the best available technology...

  19. EPA worst case water microcosms for testing phage biocontrol of Salmonella.

    PubMed

    McLaughlin, Michael R; Brooks, John P

    2008-01-01

    A microplate method was developed as a tool to test phages for their ability to control Salmonella in aqueous environments. The method used EPA (U.S. Environmental Protection Agency) worst case water (WCW) in 96-well plates. The WCW provided a consistent and relatively simple defined turbid aqueous matrix, high in total organic carbon (TOC) and total dissolved salts (TDS), to simulate swine lagoon effluent, without the inconvenience of malodor and confounding effects from other biological factors. The WCW was originally defined to simulate high turbidity and organic matter in water for testing point-of-use filtration devices. Use of WCW to simulate lagoon effluent for phage testing is a new and innovative application of this matrix. Control of physical and chemical parameters (TOC, TDS, turbidity, temperature, and pH) allowed precise evaluation of microbiological parameters (Salmonella and phages). In a typical application, wells containing WCW were loaded with Salmonella enterica susp. enterica serovar Typhimurium (ATCC14028) and treated with phages alone and in cocktail combinations. Mean Salmonella inactivation rates (k, where the lower the value, the greater the inactivation) of phage treatments ranged from -0.32 to -1.60 versus -0.004 for Salmonella controls. Mean log(10) reductions (the lower the value, the greater the reduction) of Salmonella phage treatments were -1.60 for phage PR04-1, -2.14 for phage PR37-96, and -2.14 for both phages in a sequential cocktail, versus -0.08 for Salmonella controls. The WCW microcosm system was an effective tool for evaluating the biocontrol potential of Salmonella phages.

  20. 9 CFR 166.10 - Licensing.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE SWINE HEALTH PROTECTION SWINE HEALTH PROTECTION General Provisions § 166.10 Licensing. (a) Application. Any... swine shall apply for a license on a form which will be furnished, upon request, by the Area...

  1. 9 CFR 166.10 - Licensing.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE SWINE HEALTH PROTECTION SWINE HEALTH PROTECTION General Provisions § 166.10 Licensing. (a) Application. Any... swine shall apply for a license on a form which will be furnished, upon request, by the Area...

  2. 9 CFR 166.10 - Licensing.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE SWINE HEALTH PROTECTION SWINE HEALTH PROTECTION General Provisions § 166.10 Licensing. (a) Application. Any... swine shall apply for a license on a form which will be furnished, upon request, by the Area...

  3. 9 CFR 166.10 - Licensing.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE SWINE HEALTH PROTECTION SWINE HEALTH PROTECTION General Provisions § 166.10 Licensing. (a) Application. Any... swine shall apply for a license on a form which will be furnished, upon request, by the Area...

  4. 9 CFR 166.10 - Licensing.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE SWINE HEALTH PROTECTION SWINE HEALTH PROTECTION General Provisions § 166.10 Licensing. (a) Application. Any... swine shall apply for a license on a form which will be furnished, upon request, by the Area...

  5. NITRATE CONTAMINATION OF GROUND WATER FROM LAND APPLICATION OF SWINE WASTE: CASE STUDY AND GENERAL CONSIDERATIONS

    EPA Science Inventory

    Guidelines for land application of CAFO waste may not be sufficient to prevent ground water contamination by nitrate. A case study is presented illustrating the problem for one field site disposing of swine waste. Data are discussed in context with documented land application ...

  6. Water quality improvements of wastewater from confined animal feeding operations after advanced treatment.

    PubMed

    Vanotti, Matias B; Szogi, Ariel A

    2008-01-01

    Current trends of animal production concentration and new regulations promote the need for environmentally safe alternatives to land application of liquid manure. These technologies must be able to substantially remove nutrients, heavy metals, and emissions of ammonia and odors and disinfect the effluent. A new treatment system was tested full-scale in a 4360-swine farm in North Carolina to demonstrate environmentally superior technology (EST) that could replace traditional anaerobic lagoon treatment. The system combined liquid-solids separation with nitrogen and phosphorus removal processes. Water quality was monitored at three sites: (i) the treatment plant as the raw manure liquid was depurated in the various processes, (ii) the converted lagoon as it was being cleaned up with the treated effluent, and (iii) an adjacent traditional anaerobic lagoon. The treatment plant removed 98% of total suspended solids (TSS), 76% of total solids (TS), 100% of 5-d biochemical oxygen demand (BOD(5)), 98% of total Kjeldahl nitrogen (TKN) and NH(4)-N, 95% of total phosphorus (TP), 99% of Zn, and 99% of Cu. The quality of the liquid in the converted lagoon improved rapidly as cleaner effluent from the plant replaced anaerobic lagoon liquid. The converted lagoon liquid became aerobic (dissolved oxygen, 6.95 mg L(-1); Eh, 342 mv) with the following mean reductions in the second year of the conversion: 73% of TSS, 40% of TS, 77% of BOD(5), 85% of TKN, 92% of NH(4)-N, 38% of TP, 37% of Zn, and 39% of Cu. These findings overall showed that EST can have significant positive impacts on the environment and on the livestock industries.

  7. 9 CFR 206.1 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... STOCKYARDS PROGRAMS), DEPARTMENT OF AGRICULTURE SWINE CONTRACT LIBRARY § 206.1 Definitions. The definitions... and packer rights and obligations under the contract. Base price. The price paid for swine before the application of any premiums or discounts, expressed in dollars per unit. Boar. A sexually-intact male swine...

  8. 9 CFR 206.1 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... STOCKYARDS PROGRAMS), DEPARTMENT OF AGRICULTURE SWINE CONTRACT LIBRARY § 206.1 Definitions. The definitions... and packer rights and obligations under the contract. Base price. The price paid for swine before the application of any premiums or discounts, expressed in dollars per unit. Boar. A sexually-intact male swine...

  9. 9 CFR 206.1 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... STOCKYARDS PROGRAMS), DEPARTMENT OF AGRICULTURE SWINE CONTRACT LIBRARY § 206.1 Definitions. The definitions... and packer rights and obligations under the contract. Base price. The price paid for swine before the application of any premiums or discounts, expressed in dollars per unit. Boar. A sexually-intact male swine...

  10. 9 CFR 206.1 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... STOCKYARDS PROGRAMS), DEPARTMENT OF AGRICULTURE SWINE CONTRACT LIBRARY § 206.1 Definitions. The definitions... and packer rights and obligations under the contract. Base price. The price paid for swine before the application of any premiums or discounts, expressed in dollars per unit. Boar. A sexually-intact male swine...

  11. Monitoring tylosin and sulfamethazine in a tile-drained agricultural watershed using polar organic chemical integrative sampler (POCIS).

    PubMed

    Washington, Maurice T; Moorman, Thomas B; Soupir, Michelle L; Shelley, Mack; Morrow, Amy J

    2018-01-15

    This study evaluated the influence of temporal variation on the occurrence, fate, and transport of tylosin (TYL) and sulfamethazine (SMZ); antibiotics commonly used in swine production. Atrazine (ATZ) was used as a reference analyte to indicate the agricultural origin of the antibiotics. We also assessed the impact of season and hydrology on antibiotic concentrations. A reconnaissance study of the South Fork watershed of the Iowa River (SFIR), was conducted from 2013 to 2015. Tile drain effluent and surface water were monitored using polar organic integrative sampler (POCIS) technology. Approximately 169 animal feeding operations (AFOs) exist in SFIR, with 153 of them being swine facilities. All analytes were detected, and detection frequencies ranged from 69 to 100% showing the persistence in the watershed. Antibiotics were detected at a higher frequency using POCIS compared to grab samples. We observed statistically significant seasonal trends for SMZ and ATZ concentrations during growing and harvest seasons. Time weighted average (TWA) concentrations quantified from the POCIS were 1.87ngL -1 (SMZ), 0.30ngL -1 (TYL), and 754.2ngL -1 (ATZ) in the watershed. SMZ and TYL concentrations were lower than the minimum inhibitory concentrations (MIC) for E. coli. All analytes were detected in tile drain effluent, confirming tile drainage as a pathway for antibiotic transport. Our results identify the episodic occurrence of antibiotics, and highlights the importance identifying seasonal fate and occurrence of these analytes. Published by Elsevier B.V.

  12. Ionic composition and nitrate in drainage water from fields fertilized with different nitrogen sources, middle swamp watershed, North Carolina, August 2000-August 2001

    USGS Publications Warehouse

    Harden, Stephen L.; Spruill, Timothy B.

    2004-01-01

    A study was conducted from August 2000 to August 2001 to characterize the influence of fertilizer use from different nitrogen sources on the quality of drainage water from 11 subsurface tile drains and 7 surface field ditches in a North Carolina Coastal Plain watershed. Agricultural fields receiving commercial fertilizer (conventional sites), swine lagoon effluent (spray sites), and wastewater-treatment plant sludge (sludge site) in the Middle Swamp watershed were investigated. The ionic composition of drainage water in tile drains and ditches varied depending on fertilizer source type. The dominant ions identified in water samples from tile drains and ditches include calcium, magnesium, sodium, chloride, nitrate, and sulfate, with tile drains generally having lower pH, low or no bicarbonates, and higher nitrate and chloride concentrations. Based on fertilizer source type, median nitrate-nitrogen concentrations were significantly higher at spray sites (32.0 milligrams per liter for tiles and 8.2 milligrams per liter for ditches) relative to conventional sites (6.8 milligrams per liter for tiles and 2.7 milligrams per liter for ditches). The median instantaneous nitrate-nitrogen yields also were significantly higher at spray sites (420 grams of nitrogen per hectare per day for tile drains and 15.6 grams of nitrogen per hectare per day for ditches) relative to conventional sites (25 grams of nitrogen per hectare per day for tile drains and 8.1 grams of nitrogen per hectare per day for ditches). The tile drain site where sludge is applied had a median nitrate-nitrogen concentration of 10.5 milligrams per liter and a median instantaneous nitrate-nitrogen yield of 93 grams of nitrogen per hectare per day, which were intermediate to those of the conventional and spray tile drain sites. Results from this study indicate that nitrogen loadings and subsequent edge-of-field nitrate-nitrogen yields through tile drains and ditches were significantly higher at sites receiving applications of swine lagoon effluent compared to sites receiving commercial fertilizer.

  13. Tylosin-resistant Enterococci, erm genes, and tylosin in drained fields receiving swine manure

    USDA-ARS?s Scientific Manuscript database

    The use of tylosin at subtherapeutic levels by the swine industry provides selective pressure for the development of antibiotic resistance in gastrointestinal bacteria. The land application of swine manure to drained agricultural fields might introduce elevated levels of total and tylosin-resistant ...

  14. 40 CFR 434.53 - Effluent limitations guidelines representing the degree of effluent reduction attainable by...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... representing the degree of effluent reduction attainable by application of the best available technology... representing the degree of effluent reduction attainable by application of the best available technology... application of the best available technology economically achievable: BAT Effluent Limitations Pollutant or...

  15. 40 CFR 434.53 - Effluent limitations guidelines representing the degree of effluent reduction attainable by...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... representing the degree of effluent reduction attainable by application of the best available technology... representing the degree of effluent reduction attainable by application of the best available technology... application of the best available technology economically achievable: BAT Effluent Limitations Pollutant or...

  16. 40 CFR 440.142 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... Placer Mine Subcategory § 440.142 Effluent limitations representing the degree of effluent reduction attainable by the application of the best practicable control technology currently available (BPT). Except as...

  17. 40 CFR 440.142 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... Placer Mine Subcategory § 440.142 Effluent limitations representing the degree of effluent reduction attainable by the application of the best practicable control technology currently available (BPT). Except as...

  18. 40 CFR 434.53 - Effluent limitations guidelines representing the degree of effluent reduction attainable by...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... representing the degree of effluent reduction attainable by application of the best available technology... representing the degree of effluent reduction attainable by application of the best available technology... application of the best available technology economically achievable: BAT Effluent Limitations Pollutant or...

  19. 40 CFR 434.53 - Effluent limitations guidelines representing the degree of effluent reduction attainable by...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by application of the best available technology... representing the degree of effluent reduction attainable by application of the best available technology... application of the best available technology economically achievable: BAT Effluent Limitations Pollutant or...

  20. 40 CFR 440.142 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... Placer Mine Subcategory § 440.142 Effluent limitations representing the degree of effluent reduction attainable by the application of the best practicable control technology currently available (BPT). Except as...

  1. 40 CFR 408.202 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... the degree of effluent reduction attainable by the application of the best practicable control... effluent reduction attainable by the application of the best practicable control technology currently...

  2. 40 CFR 408.172 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... representing the degree of effluent reduction attainable by the application of the best practicable control... effluent reduction attainable by the application of the best practicable control technology currently...

  3. 40 CFR 408.172 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... representing the degree of effluent reduction attainable by the application of the best practicable control... effluent reduction attainable by the application of the best practicable control technology currently...

  4. 40 CFR 408.162 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... representing the degree of effluent reduction attainable by the application of the best practicable control... effluent reduction attainable by the application of the best practicable control technology currently...

  5. 40 CFR 408.202 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... the degree of effluent reduction attainable by the application of the best practicable control... effluent reduction attainable by the application of the best practicable control technology currently...

  6. 40 CFR 408.312 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... representing the degree of effluent reduction attainable by the application of the best practicable control... effluent reduction attainable by the application of the best practicable control technology currently...

  7. 40 CFR 408.162 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... representing the degree of effluent reduction attainable by the application of the best practicable control... effluent reduction attainable by the application of the best practicable control technology currently...

  8. 40 CFR 408.312 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... representing the degree of effluent reduction attainable by the application of the best practicable control... effluent reduction attainable by the application of the best practicable control technology currently...

  9. 40 CFR 408.162 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... representing the degree of effluent reduction attainable by the application of the best practicable control... effluent reduction attainable by the application of the best practicable control technology currently...

  10. 40 CFR 408.202 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... the degree of effluent reduction attainable by the application of the best practicable control... effluent reduction attainable by the application of the best practicable control technology currently...

  11. 40 CFR 408.312 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... representing the degree of effluent reduction attainable by the application of the best practicable control... effluent reduction attainable by the application of the best practicable control technology currently...

  12. 40 CFR 408.312 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... representing the degree of effluent reduction attainable by the application of the best practicable control... effluent reduction attainable by the application of the best practicable control technology currently...

  13. 40 CFR 408.172 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... representing the degree of effluent reduction attainable by the application of the best practicable control... effluent reduction attainable by the application of the best practicable control technology currently...

  14. 40 CFR 408.202 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... the degree of effluent reduction attainable by the application of the best practicable control... effluent reduction attainable by the application of the best practicable control technology currently...

  15. 40 CFR 408.202 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... the degree of effluent reduction attainable by the application of the best practicable control... effluent reduction attainable by the application of the best practicable control technology currently...

  16. 40 CFR 408.312 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... representing the degree of effluent reduction attainable by the application of the best practicable control... effluent reduction attainable by the application of the best practicable control technology currently...

  17. Occurrence and transport of tetracycline, sulfonamide, quinolone, and macrolide antibiotics in the Haihe River Basin, China.

    PubMed

    Luo, Yi; Xu, Lin; Rysz, Michal; Wang, Yuqiu; Zhang, Hao; Alvarez, Pedro J J

    2011-03-01

    The occurrence and transport of 12 antibiotics (from the tetracycline, sulfonamide, quinolone, and macrolide families) was studied in a 72-km stretch of the Haihe River, China, and in six of its tributaries. Aqueous and sediment samples were analyzed by HPLC-MS/MS. Sulfonamides were detected at the highest concentrations (24-385 ng/L) and highest frequencies (76-100%). Eight of the 12 antibiotics likely originated from veterinary applications in swine farms and fishponds, and concentrations at these sources (0.12-47 μg/L) were 1-2 orders of magnitude higher than in the effluent of local wastewater treatment plants. Sulfachloropyridazine (SCP) was detected in all swine farm and fishpond samples (maximum concentration 47 μg/L), which suggests its potential usefulness to indicate livestock source pollution in the Haihe River basin. Hydrological and chemical factors that may influence antibiotic distribution in the Haihe River were considered by multiple regression analysis. River flow rate exerted the most significant effect on the first-order attenuation coefficient (K) for sulfonamides, quinolones, and macrolides, with higher flow rates resulting in higher K, probably due to dilution. For tetracyclines, sediment total organic matter and cation exchange capacity exerted a greater impact on K than flow rate, indicating that adsorption to sediments plays an important role in attenuating tetracycline migration. Overall, the predominance of sulfonamides in the Haihe River underscores the need to consider regulating their veterinary use and improving the management and treatment of associated releases.

  18. Source estimation of pharmaceuticals based on catchment population and in-stream attenuation in Yodo River watershed, Japan.

    PubMed

    Hanamoto, Seiya; Nakada, Norihide; Yamashita, Naoyuki; Tanaka, Hiroaki

    2018-02-15

    Fifty-five pharmaceuticals were monitored at four rivers and inlets and/or outlets of three sewage treatment plants (STPs) in Yodo River watershed, Japan over 17 sampling events. Twenty-six quantified pharmaceuticals were classified by source and fate. The load per person (LPP) of nine pharmaceuticals, including six with observed mass balance in studied river stretch of <80%, was appreciably lower in river water (RW) than in the effluent (EF) of STPs (RW/EF <0.5), indicating that they were susceptible to in-stream attenuation in the study area, while the others were relatively conservative. The LPP of 12 pharmaceuticals in RW were within ±50% of that in EF. Because their mass loadings in rivers were correlated with human population in the catchment and most people use the sewer system, the major source of the 12 pharmaceuticals was considered to be STPs. The LPP of the three most labile pharmaceuticals in STPs (caffeine, theophylline, and acetaminophen) was >1.5 in RW/EF and <1.0 in RW/influent (IF) of STPs. Poorly treated sewage discharged from households without using the sewer system was considered to be influential source of the three pharmaceuticals. The LPP (RW/EF) of caffeine, a pharmaceutical contained in food and beverage, was considerably higher than that of the other two, and this is attributable to untreated gray water discharged at households using the night-soil treatment system. The LPP of two veterinary drugs (sulfamonomethoxine and lincomycin) were >1.5 (RW/EF) and >1.0 (RW/IF). Their mass loadings in rivers showed a positive correlation with swine population in the catchment, although sulfamonomethoxine is equally used in both cattle and swine farming. This was attributable to application of cattle excrement as manure, and lability of sulfamonomethoxine during composting processes. The major source of the two veterinary drugs was considered to be on-site treatment facilities of swine urine. Copyright © 2017 Elsevier B.V. All rights reserved.

  19. 40 CFR 412.40 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... CONCENTRATED ANIMAL FEEDING OPERATIONS (CAFO) POINT SOURCE CATEGORY Swine, Poultry, and Veal Calves § 412.40...) under 40 CFR 122.23 and includes the following animals: swine; chickens; turkeys; and veal calves. This subpart does not apply to such CAFOs with less than the following capacities: 2,500 swine each weighing 55...

  20. 40 CFR 412.40 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... CONCENTRATED ANIMAL FEEDING OPERATIONS (CAFO) POINT SOURCE CATEGORY Swine, Poultry, and Veal Calves § 412.40...) under 40 CFR 122.23 and includes the following animals: swine; chickens; turkeys; and veal calves. This subpart does not apply to such CAFOs with less than the following capacities: 2,500 swine each weighing 55...

  1. 40 CFR 412.40 - Applicability.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... CONCENTRATED ANIMAL FEEDING OPERATIONS (CAFO) POINT SOURCE CATEGORY Swine, Poultry, and Veal Calves § 412.40...) under 40 CFR 122.23 and includes the following animals: swine; chickens; turkeys; and veal calves. This subpart does not apply to such CAFOs with less than the following capacities: 2,500 swine each weighing 55...

  2. Technique Development for a Polytrauma Model to Study Partial Resuscitative Endovascular Balloon Occlusion of the Aorta (P-REBOA) in Swine (Sus scrofa)

    DTIC Science & Technology

    2016-06-01

    widely in literature, limiting comparisons. Methods: Yorkshire-cross swine were anesthetized, instrumented, and splenectomized. A simple liver...applicable injury in swine . Use of the tourniquet allowed for consistent liver injury and precise control over hemorrhage.

  3. Occurrence and movement of antibiotic resistant bacxteria, in tile-drained agricultural fields receiving swine manure

    USDA-ARS?s Scientific Manuscript database

    The use of tylosin at subtherapeutic levels by the swine industry provides selective pressure for the development of antibiotic resistance in gastrointestinal bacteria. The land application of swine manure to drained agricultural fields might accelerate the transport of pathogen indicators such as e...

  4. Soil application of various biochars produced from both dry and wet pyrolysis

    USDA-ARS?s Scientific Manuscript database

    The objectives of this study were to 1) compare physico-chemical and thermal characteristics of swine manure-based hyrdochar and pyrochar, and 2) investigate greenhouse gas emission and groundwater pollution potentials of the swine hydrochar when used as a soil amendment. Dewatered swine solids wer...

  5. 40 CFR 430.112 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... effluent reduction attainable by the application of the best practicable control technology currently... reduction attainable by the application of the best practicable control technology currently available (BPT...

  6. 40 CFR 430.112 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... effluent reduction attainable by the application of the best practicable control technology currently... reduction attainable by the application of the best practicable control technology currently available (BPT...

  7. Detection of hepatitis E virus and other livestock-related pathogens in Iowa streams

    USGS Publications Warehouse

    Givens, Carrie E.; Kolpin, Dana W.; Borchardt, Mark A.; Duris, Joseph W.; Moorman, Thomas B.; Spencer, Susan K.

    2016-01-01

    Manure application is a source of pathogens to the environment. Through overland runoff and tile drainage, zoonotic pathogens can contaminate surface water and streambed sediment and could affect both wildlife and human health. This study examined the environmental occurrence of gene markers for livestock-related bacterial, protozoan, and viral pathogens and antibiotic resistance in surface waters within the South Fork Iowa River basin before and after periods of swine manure application on agricultural land. Increased concentrations of indicator bacteria after manure application exceeding Iowa's state bacteria water quality standards suggest that swine manure contributes to diminished water quality and may pose a risk to human health. Additionally, the occurrence of HEV and numerous bacterial pathogen genes for Escherichia coli, Enterococcus spp., Salmonella sp., and Staphylococcus aureus in both manure samples and in corresponding surface water following periods of manure application suggests a potential role for swine in the spreading of zoonotic pathogens to the surrounding environment. During this study, several zoonotic pathogens were detected including Shiga-toxin producing E. coli, Campylobacter jejuni, pathogenic enterococci, and S. aureus; all of which can pose mild to serious health risks to swine, humans, and other wildlife. This research provides the foundational understanding required for future assessment of the risk to environmental health from livestock-related zoonotic pathogen exposures in this region. This information could also be important for maintaining swine herd biosecurity and protecting the health of wildlife near swine facilities.

  8. Zinc and copper distribution in swine wastewater treated by anaerobic digestion.

    PubMed

    Cestonaro do Amaral, André; Kunz, Airton; Radis Steinmetz, Ricardo Luís; Justi, Karin Cristiane

    2014-08-01

    Swine wastewater contain high levels of metals, such as copper and zinc, which can cause a negative impact on the environment. Anaerobic digestion is a process commonly used to remove carbon, and can act on metal availability (e.g., solubility or oxidation state). The present study aimed to evaluate the influence of anaerobic digestion on total Zn and Cu contents, and their chemical fractioning due to the biodegradation of the effluent over different hydraulic retention times (HRTs). The sequential extraction protocol proposed by the Community Bureau of Reference (BCR), plus two additional fractions, was the method chosen for this study of Cu and Zn distribution evaluation in swine wastewater. The Zn and Cu concentrations in raw swine manure were 63.58 ± 27.72 mg L(-1) and 8.98 ± 3.99 mg L(-1), respectively. The metal retention capacity of the bioreactor decreased when the HRT was reduced from 17.86 d to 5.32 d. Anaerobic digestion had a direct influence on zinc and copper distribution when raw manure (RM) and digested manure (DM) were compared. The reducible fraction showed a reduction of between 3.17% and 7.84% for Zn and between 2.52% and 11.92% for Cu when DM was compared with RM. However, the metal concentration increased in the oxidizable fraction of DM, viz. from 3.01% to 10.64% for Zn and from 4.49% to 16.71% for Cu, thus demonstrating the effect of anaerobic conditions on metal availability. Copyright © 2014 Elsevier Ltd. All rights reserved.

  9. Forage and tree seedling growth in a soil with an encased swine sludge layer.

    PubMed

    Penn, Chad J; Will, Rodney; Fultz, Lisa; Hamilton, Doug

    2013-10-15

    The closure of swine farms requires decommissioning of lagoons that contain large amounts of swine solids (sludge). Sludge is typically transported and land applied to soils. However, in some cases this process could be economically prohibitive and/or unpractical. An alternative idea is to encase sludge with lagoon soil berms after removing overlying effluent, followed by establishment of forages or short-rotation woody crops on the encased sludge. The objective of this study was to investigate growth potential for several forages and tree species into a pure layer of swine sludge. Alfalfa (Meticago sativa), bermudagrass (Cynodon dactylon), switchgrass (Panicum virgatum), green ash (Fraxinus pennsylvanica), black locust (Robinia pseudoacacia), and sycamore (Platanus occidentalis) were established in 40 cm deep pots consisting of a lagoon berm soil overlaying a sludge layer for 12 w followed by analysis of aboveground and belowground biomass production. "New" and "old" sludge was collected from an active 10 year old lagoon and decommissioned 50 year old lagoon, respectively. A control (soil only) was used. Encased sludge treatments increased forage biomass production. Sycamore and green ash were sensitive to new sludge but not old sludge as these species had less biomass production in new sludge than control and showed tissue trace nutrient deficiencies. While both sludge materials contained adequate nutrients, the new sludge had a salt concentration 1.8 times higher than old sludge as indicated by electrical conductivity (12.4 mS). Thus, the forage crops and black locust were able to thrive in new sludge due to their salt tolerance. Copyright © 2013 Elsevier Ltd. All rights reserved.

  10. 9 CFR 149.3 - Site audit.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    .... as follows: (1) The movement of all non-breeding swine 5 weeks of age or older into or from the pork... previous site (if applicable). (2) All non-breeding swine entering a site must have originated from another certified production site, except that non-breeding swine less than 5 weeks of age may have originated from...

  11. 9 CFR 149.3 - Site audit.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    .... as follows: (1) The movement of all non-breeding swine 5 weeks of age or older into or from the pork... previous site (if applicable). (2) All non-breeding swine entering a site must have originated from another certified production site, except that non-breeding swine less than 5 weeks of age may have originated from...

  12. 9 CFR 149.3 - Site audit.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    .... as follows: (1) The movement of all non-breeding swine 5 weeks of age or older into or from the pork... previous site (if applicable). (2) All non-breeding swine entering a site must have originated from another certified production site, except that non-breeding swine less than 5 weeks of age may have originated from...

  13. 77 FR 22789 - Withdrawal of Approval of Part of a New Animal Drug Application; Tiamulin

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-17

    ... in swine. This action is being taken at the sponsor's request because this product is no longer... feed efficiency in swine. Novartis requested voluntary withdrawal of approval of these indications for... indications for use of increased rate of weight gain and improved feed efficiency in swine are hereby...

  14. Reconnaissance of Water Quality at Four Swine Farms in Jackson County, Florida, 1993

    DTIC Science & Technology

    1996-01-01

    applications on agricultural land. (Krider, 1987). Since the estimated annual wet manure product in pounds per animal is: 3,407 for breeding swine , and...Reconnaissance of Water Quality at Four Swine Farms in Jackson County, Florida, 1993 By Jerilyn J. Collins U.S. Geological Survey Open File Report...COVERED - 4. TITLE AND SUBTITLE Reconnaissance of Water Quality at Four Swine Farms in Jackson County, Florida, 1993 5a. CONTRACT NUMBER 5b. GRANT

  15. Fate and transport of tylosin and macrolide-resistance genes following manure applications in tile-drained landscapes

    USDA-ARS?s Scientific Manuscript database

    The use of antibiotics in swine production leads to antibiotic-resistance in gastrointestinal bacteria. Application of swine manure to drained agricultural fields introduces elevated levels of antibiotic-resistant bacteria and residual antibiotics. The persistence and transport of these agents are g...

  16. Solids, organic load and nutrient concentration reductions in swine waste slurry using a polyacrylamide (PAM)-aided solids flocculation treatment.

    PubMed

    Walker, Paul; Kelley, Tim

    2003-11-01

    Increased swine production results in concentration of wastes generated within a limited geographical area, which may lead to land application rates exceeding the local or regional assimilatory capacity. This may result in pollutant transfer through surface water or soil-groundwater systems, environmental degradation, and/or odor concerns. Existing swine waste pit storage and lagoon treatment technologies may be inadequate to store or treat waste prior to land application without these concerns resulting. Efficient swine waste solids separation may reduce environmental health concerns and generate a value-added bioresource (solids). This study evaluated the efficiency of a polyacrylamide (PAM) flocculant-aided solids separation treatment to reduce pollution indicator concentrations in raw (untreated) swine waste slurry. Swine waste slurry solids separation efficiency through gravity settling (sedimentation) was evaluated before and after the addition of a proprietary polymeric (PAM) flocculant. Results indicated that polymer amendments at concentrations of 62.5-750 mg/l improved slurry solids separation efficiency and significantly reduced concentrations of other associated aquatic pollution indicators in a majority of analyses conducted (33 of 50 total analyses conducted). Results also suggested that PAM-aided solids separation from swine waste slurry might facilitate further treatment and/or disposal and therefore reduce associated environmental degradation potential.

  17. Impacts of swine manure application and land management on microbial communities and resistance genes in soils and drainage waters

    USDA-ARS?s Scientific Manuscript database

    Administration of in-feed antibiotics have been observed to lead to increased concentrations of antibiotics and antibiotic resistant bacteria in swine manure. Since swine manure is often used as a fertilizer in agriculture, there is concern that antibiotics and resistance genes introduced into the a...

  18. 40 CFR 429.63 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TIMBER PRODUCTS PROCESSING POINT SOURCE CATEGORY Wet Process... by the application of the best available technology economically achievable (BAT). [Reserved] ...

  19. 40 CFR 430.24 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... new technology components or process modifications and identifying critical path relationships within... degree of effluent reduction attainable by the application of best available technology economically... effluent reduction attainable by the application of best available technology economically achievable (BAT...

  20. Struvite recovery from swine waste biogas digester effluent through a stainless steel device under constant pH conditions.

    PubMed

    Perera, P W Anton; Wu, Wei-Xiang; Chen, Ying-Xu; Han, Zhi-Ying

    2009-06-01

    To investigate the struvite precipitation under constant and non-constant pH conditions and to test a stainless steel device under different operating regimes to maximize the recovery of struvite. The molar ratio of NH4+: Mg2+: PO4(3-) was adjusted to 1: 1.2: 1.2 and pH was elevated to 9.0. The absorbance measurement was used to trace the process of struvite crystallization. Wastewater and precipitate analysis was done by standard analytical methods. The pH constant experiment reported a significantly higher struvite precipitation (24.6 +/- 0.86 g) than the non-constant pH experiment (19.8 +/- 1.86 g). The SAR ranged from 5.6 to 8.2 g m(-2) h(-1) to 3.6-4.8 g m(-2) h(-1) in pH constant and non-constant experiments, respectively. The highest struvite deposit on the device was found in regime 3 followed by in regimes 2 and 4. The highest PO4(3-) (97.2%) and NH4+ (71%) removal was reported in the R1 regime. None of the influent Cu2+ or Zn2+ was precipitated on the device. A higher struvite yield is evident in pH constant experiments. Moreover, the stainless steel device facilitates the isolation of heavy metal free pure (around 96%) struvite from swine waste biogas digester effluent contaminated with cu2+ and Zn2+ and the highest yield is attainable with the device operating at 50 rpm with agitation by a magnetic stirrer.

  1. 40 CFR 469.15 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically...) EFFLUENT GUIDELINES AND STANDARDS ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE CATEGORY Semiconductor... application of the best available technology economically achievable (BAT). Except as provided in 40 CFR 125...

  2. 40 CFR 469.24 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE CATEGORY... attainable by the application of the best practicable control technology currently available (BPT). Except as...

  3. 40 CFR 469.14 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE CATEGORY... by the application of the best practicable control technology currently available (BPT). Except as...

  4. 40 CFR 469.25 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically...) EFFLUENT GUIDELINES AND STANDARDS ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE CATEGORY Electronic... by the application of the best available technology economically achievable (BAT). Except as provided...

  5. 40 CFR 418.73 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... effluent reduction attainable by the application of the best available technology economically achievable... subpart after application of the best available technology economically achievable: There shall be no...

  6. 40 CFR 429.51 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... application of the best practicable control technology (BPT): There shall be no discharge of process... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TIMBER PRODUCTS PROCESSING POINT SOURCE CATEGORY Dry Process...

  7. 40 CFR 408.292 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... degree of effluent reduction attainable by the application of the best practicable control technology... reduction attainable by the application of the best practicable control technology currently available (BPT...

  8. 40 CFR 408.292 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... degree of effluent reduction attainable by the application of the best practicable control technology... reduction attainable by the application of the best practicable control technology currently available (BPT...

  9. 40 CFR 408.292 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... degree of effluent reduction attainable by the application of the best practicable control technology... reduction attainable by the application of the best practicable control technology currently available (BPT...

  10. 40 CFR 408.292 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... degree of effluent reduction attainable by the application of the best practicable control technology... reduction attainable by the application of the best practicable control technology currently available (BPT...

  11. 40 CFR 408.292 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... degree of effluent reduction attainable by the application of the best practicable control technology... reduction attainable by the application of the best practicable control technology currently available (BPT...

  12. 40 CFR 429.163 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TIMBER PRODUCTS PROCESSING POINT SOURCE CATEGORY Wood Furniture... application of the best available technology economically achievable (BAT). Except as provided in 40 CFR 125...

  13. 40 CFR 429.161 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TIMBER PRODUCTS PROCESSING POINT SOURCE CATEGORY Wood Furniture... application of the best practicable control technology currently available (BPT). Except as provided in 40 CFR...

  14. 40 CFR 410.42 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TEXTILE MILLS POINT SOURCE CATEGORY Woven Fabric Finishing... application of the best practicable control technology currently available (BPT). (a) Except as provided in 40...

  15. 40 CFR 410.82 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TEXTILE MILLS POINT SOURCE CATEGORY Nonwoven Manufacturing... application of the best practicable control technology currently available (BPT). Except as provided in 40 CFR...

  16. 40 CFR 410.92 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TEXTILE MILLS POINT SOURCE CATEGORY Felted Fabric Processing... application of the best practicable control technology currently available (BPT). Except as provided in 40 CFR...

  17. 40 CFR 410.62 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TEXTILE MILLS POINT SOURCE CATEGORY Carpet Finishing... application of the best practicable control technology currently available (BPT). (a) Except as provided in 40...

  18. 40 CFR 410.12 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TEXTILE MILLS POINT SOURCE CATEGORY Wool Scouring Subcategory... application of the best practicable control technology currently available (BPT). (a) Except as provided in 40...

  19. 40 CFR 410.22 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TEXTILE MILLS POINT SOURCE CATEGORY Wool Finishing Subcategory... application of the best practicable control technology currently available (BPT). (a) Except as provided in 40...

  20. 40 CFR 468.16 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 30 2011-07-01 2011-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best conventional pollution control... application of the best conventional pollution control technology (BCT). [Reserved] ...

  1. 40 CFR 468.16 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best conventional pollution control... application of the best conventional pollution control technology (BCT). [Reserved] ...

  2. 40 CFR 469.15 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically...) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE CATEGORY... by the application of the best available technology economically achievable (BAT). Except as provided...

  3. 40 CFR 469.25 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically...) EFFLUENT GUIDELINES AND STANDARDS ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE CATEGORY Electronic... by the application of the best available technology economically achievable (BAT). Except as provided...

  4. 40 CFR 430.114 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... representing the degree of effluent reduction attainable by the application of the best available technology... best available technology economically achievable (BAT). Non-continuous dischargers shall not be...

  5. 40 CFR 469.15 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically...) EFFLUENT GUIDELINES AND STANDARDS ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE CATEGORY Semiconductor... application of the best available technology economically achievable (BAT). Except as provided in 40 CFR 125...

  6. 40 CFR 469.14 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE... reduction attainable by the application of the best practicable control technology currently available (BPT...

  7. 40 CFR 469.24 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE... reduction attainable by the application of the best practicable control technology currently available (BPT...

  8. 40 CFR 469.24 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE... reduction attainable by the application of the best practicable control technology currently available (BPT...

  9. 40 CFR 469.25 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically...) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE CATEGORY... attainable by the application of the best available technology economically achievable (BAT). Except as...

  10. 40 CFR 469.24 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE... reduction attainable by the application of the best practicable control technology currently available (BPT...

  11. 40 CFR 469.14 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE... reduction attainable by the application of the best practicable control technology currently available (BPT...

  12. 40 CFR 469.14 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE... reduction attainable by the application of the best practicable control technology currently available (BPT...

  13. 40 CFR 469.25 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically...) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE CATEGORY... attainable by the application of the best available technology economically achievable (BAT). Except as...

  14. 40 CFR 469.24 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE CATEGORY... attainable by the application of the best practicable control technology currently available (BPT). Except as...

  15. 40 CFR 469.15 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically...) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE CATEGORY... by the application of the best available technology economically achievable (BAT). Except as provided...

  16. 40 CFR 469.14 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE CATEGORY... by the application of the best practicable control technology currently available (BPT). Except as...

  17. 40 CFR 468.16 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 31 2013-07-01 2013-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best conventional pollution control... application of the best conventional pollution control technology (BCT). [Reserved] ...

  18. 40 CFR 468.16 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 31 2012-07-01 2012-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best conventional pollution control... application of the best conventional pollution control technology (BCT). [Reserved] ...

  19. 40 CFR 468.16 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 30 2014-07-01 2014-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best conventional pollution control... application of the best conventional pollution control technology (BCT). [Reserved] ...

  20. 40 CFR 455.64 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS PESTICIDE CHEMICALS Repackaging of Agricultural Pesticides... effluent reduction attainable by the application of the best available technology economically achievable...

  1. In-situ pyrogenic production of biodiesel from swine fat.

    PubMed

    Lee, Jechan; Tsang, Yiu Fai; Jung, Jong-Min; Oh, Jeong-Ik; Kim, Hyung-Wook; Kwon, Eilhann E

    2016-11-01

    In-situ production of fatty acid methyl esters from swine fat via thermally induced pseudo-catalytic transesterification on silica was investigated in this study. Instead of methanol, dimethyl carbonate (DMC) was used as acyl acceptor to achieve environmental benefits and economic viability. Thermo-gravimetric analysis of swine fat reveals that swine fat contains 19.57wt.% of water and impurities. Moreover, the fatty acid profiles obtained under various conditions (extracted swine oil+methanol+NaOH, extracted swine oil+DMC+pseudo-catalytic, and swine fat+DMC+pseudo-catalytic) were compared. These profiles were identical, showing that the introduced in-situ transesterification is technically feasible. This also suggests that in-situ pseudo-catalytic transesterification has a high tolerance against impurities. This study also shows that FAME yield via in-situ pseudo-catalytic transesterification of swine fat reached up to 97.2% at 380°C. Therefore, in-situ pseudo-catalytic transesterification can be applicable to biodiesel production of other oil-bearing biomass feedstocks. Copyright © 2016 Elsevier Ltd. All rights reserved.

  2. 40 CFR 424.13 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS FERROALLOY MANUFACTURING POINT SOURCE CATEGORY Open Electric... representing the degree of effluent reduction attainable by the application of the best available technology...

  3. 40 CFR 458.23 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... Thermal Process Subcategory § 458.23 Effluent limitations guidelines representing the degree of effluent reduction attainable by the application of the best available technology economically achievable. The...

  4. 40 CFR 458.33 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... Channel Process Subcategory § 458.33 Effluent limitations guidelines representing the degree of effluent reduction attainable by the application of the best available technology economically achievable. The...

  5. 40 CFR 458.13 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... Furnace Process Subcategory § 458.13 Effluent limitations guidelines representing the degree of effluent reduction attainable by the application of the best available technology economically achievable. The...

  6. 40 CFR 420.137 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best control technology for... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS IRON AND STEEL MANUFACTURING POINT SOURCE CATEGORY... effluent reduction attainable by the application of the best control technology for conventional pollutants...

  7. 40 CFR 434.43 - Effluent limitations guidelines representing the degree of effluent reduction attainable by...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by application of the best available technology... NEW SOURCE PERFORMANCE STANDARDS Alkaline Mine Drainage § 434.43 Effluent limitations guidelines representing the degree of effluent reduction attainable by application of the best available technology...

  8. 40 CFR 434.43 - Effluent limitations guidelines representing the degree of effluent reduction attainable by...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... representing the degree of effluent reduction attainable by application of the best available technology... NEW SOURCE PERFORMANCE STANDARDS Alkaline Mine Drainage § 434.43 Effluent limitations guidelines representing the degree of effluent reduction attainable by application of the best available technology...

  9. 40 CFR 424.13 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS FERROALLOY MANUFACTURING POINT SOURCE CATEGORY Open Electric... representing the degree of effluent reduction attainable by the application of the best available technology...

  10. 40 CFR 424.13 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS FERROALLOY MANUFACTURING POINT SOURCE CATEGORY Open Electric... representing the degree of effluent reduction attainable by the application of the best available technology...

  11. 40 CFR 424.13 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS FERROALLOY MANUFACTURING POINT SOURCE CATEGORY Open Electric... representing the degree of effluent reduction attainable by the application of the best available technology...

  12. 40 CFR 424.13 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS FERROALLOY MANUFACTURING POINT SOURCE CATEGORY Open Electric... representing the degree of effluent reduction attainable by the application of the best available technology...

  13. 40 CFR 463.12 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS PLASTICS MOLDING AND FORMING POINT SOURCE CATEGORY... degree of effluent reduction attainable by the application of the best practicable control technology...

  14. 40 CFR 463.17 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best conventional pollutant... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS PLASTICS MOLDING AND FORMING POINT SOURCE CATEGORY Contact... effluent reduction attainable by the application of the best conventional pollutant control technology...

  15. 40 CFR 463.13 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS PLASTICS MOLDING AND FORMING POINT SOURCE CATEGORY Contact... effluent reduction attainable by the application of the best available technology economically achievable...

  16. Narrow grass hedge effects on nutrient transport following swine slurry application

    USDA-ARS?s Scientific Manuscript database

    The effectiveness of a narrow grass hedge in reducing runoff nutrient loads following swine slurry application was examined in this study. Slurry was applied to 0.75-m wide by 4.0-m long plots established on an Aksarben silty clay loam soil located in southeast Nebraska. Manure treatments consisted ...

  17. 40 CFR 440.142 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORE MINING AND DRESSING POINT SOURCE CATEGORY Gold Placer Mine... application of the best practicable control technology currently available (BPT). Except as provided in 40 CFR...

  18. 40 CFR 410.72 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TEXTILE MILLS POINT SOURCE CATEGORY Stock and Yarn Finishing... application of the best practicable control technology currently available (BPT). (a) Except as provided in 40...

  19. 40 CFR 469.19 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best conventional pollution control... application of the best conventional pollution control technology (BCT). Except as provided in 40 CFR 125.30... conventional pollution control technology (BCT): Subpart A—Semiconductor BCT Effluent Limitations Pollutant or...

  20. 40 CFR 430.112 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... technology currently available (BPT). Except as provided in 40 CFR 125.30 through 125.32, any existing point... effluent reduction attainable by the application of the best practicable control technology currently...

  1. 40 CFR 430.112 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... technology currently available (BPT). Except as provided in 40 CFR 125.30 through 125.32, any existing point... effluent reduction attainable by the application of the best practicable control technology currently...

  2. 40 CFR 430.112 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... technology currently available (BPT). Except as provided in 40 CFR 125.30 through 125.32, any existing point... effluent reduction attainable by the application of the best practicable control technology currently...

  3. Bacterial community dynamics in surface flow constructed wetlands for the treatment of swine waste.

    PubMed

    Ibekwe, A M; Ma, J; Murinda, Shelton; Reddy, G B

    2016-02-15

    Constructed wetlands are generally used for the removal of waste from contaminated water. In the swine production system, wastes are traditionally flushed into an anaerobic lagoon which is then sprayed on agricultural fields. However, continuous spraying of lagoon wastewater on fields can lead to high N and P accumulations in soil or lead to runoff which may contaminate surface or ground water with pathogens and nutrients. In this study, continuous marsh constructed wetland was used for the removal of contaminants from swine waste. Using pyrosequencing, we assessed bacterial composition within the wetland using principal coordinate analysis (PCoA) which showed that bacterial composition from manure influent and lagoon water were significantly different (P=0.001) from the storage pond to the final effluent. Canonical correspondence analysis (CCA) showed that different bacterial populations were significantly impacted by ammonium--NH4 (P=0.035), phosphate--PO4(3-) (P=0.010), chemical oxygen demand--COD (P=0.0165), total solids--TS (P=0.030), and dissolved solids--DS (P=0.030) removal, with 54% of the removal rate explained by NH4+PO4(3-) according to a partial CCA. Our results showed that different bacterial groups were responsible for the composition of different wetland nutrients and decomposition process. This may be the major reason why most wetlands are very efficient in waste decomposition. Published by Elsevier B.V.

  4. 40 CFR 440.142 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORE MINING AND DRESSING POINT SOURCE CATEGORY Gold Placer Mine... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best practicable control technology...

  5. 40 CFR 440.105 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best conventional pollutant control... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORE MINING AND DRESSING POINT SOURCE CATEGORY Copper...

  6. 40 CFR 429.153 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). [Reserved] 429.153 Section 429.153 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES...

  7. 40 CFR 430.84 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of best available technology economically achievable (BAT). [Reserved] 430.84 Section 430.84 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND...

  8. 40 CFR 415.332 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... SOURCE CATEGORY Carbon Monoxide and By-Product Hydrogen Production Subcategory § 415.332 Effluent limitations guidelines representing the degree of effluent reduction attainable by the application of the best... representing the degree of effluent reduction attainable by the application of the best practicable control...

  9. Dual application of duckweed and azolla plants for wastewater treatment and renewable fuels and petrochemicals production

    PubMed Central

    2014-01-01

    Background Shortages in fresh water supplies today affects more than 1 billion people worldwide. Phytoremediation strategies, based on the abilities of aquatic plants to recycle nutrients offer an attractive solution for the bioremediation of water pollution and represents one of the most globally researched issues. The subsequent application of the biomass from the remediation for the production of fuels and petrochemicals offers an ecologically friendly and cost-effective solution for water pollution problems and production of value-added products. Results In this paper, the feasibility of the dual application of duckweed and azolla aquatic plants for wastewater treatment and production of renewable fuels and petrochemicals is explored. The differences in absorption rates of the key wastewater nutrients, ammonium and phosphorus by these aquatic macrophytes were used as the basis for optimization of the composition of wastewater effluents. Analysis of pyrolysis products showed that azolla and algae produce a similar range of bio-oils that contain a large spectrum of petrochemicals including straight-chain C10-C21 alkanes, which can be directly used as diesel fuel supplement, or a glycerin-free component of biodiesel. Pyrolysis of duckweed produces a different range of bio-oil components that can potentially be used for the production of “green” gasoline and diesel fuel using existing techniques, such as catalytic hydrodeoxygenation. Conclusions Differences in absorption rates of the key wastewater nutrients, ammonium and phosphorus by different aquatic macrophytes can be used for optimization of composition of wastewater effluents. The generated data suggest that the composition of the petrochemicals can be modified in a targeted fashion, not only by using different species, but also by changing the source plants’ metabolic profile, by exposing them to different abiotic or biotic stresses. This study presents an attractive, ecologically friendly and cost-effective solution for efficient bio-filtration of swine wastewater and petrochemicals production from generated biomass. PMID:24576349

  10. Dual application of duckweed and azolla plants for wastewater treatment and renewable fuels and petrochemicals production.

    PubMed

    Muradov, Nazim; Taha, Mohamed; Miranda, Ana F; Kadali, Krishna; Gujar, Amit; Rochfort, Simone; Stevenson, Trevor; Ball, Andrew S; Mouradov, Aidyn

    2014-02-28

    Shortages in fresh water supplies today affects more than 1 billion people worldwide. Phytoremediation strategies, based on the abilities of aquatic plants to recycle nutrients offer an attractive solution for the bioremediation of water pollution and represents one of the most globally researched issues. The subsequent application of the biomass from the remediation for the production of fuels and petrochemicals offers an ecologically friendly and cost-effective solution for water pollution problems and production of value-added products. In this paper, the feasibility of the dual application of duckweed and azolla aquatic plants for wastewater treatment and production of renewable fuels and petrochemicals is explored. The differences in absorption rates of the key wastewater nutrients, ammonium and phosphorus by these aquatic macrophytes were used as the basis for optimization of the composition of wastewater effluents. Analysis of pyrolysis products showed that azolla and algae produce a similar range of bio-oils that contain a large spectrum of petrochemicals including straight-chain C10-C21 alkanes, which can be directly used as diesel fuel supplement, or a glycerin-free component of biodiesel. Pyrolysis of duckweed produces a different range of bio-oil components that can potentially be used for the production of "green" gasoline and diesel fuel using existing techniques, such as catalytic hydrodeoxygenation. Differences in absorption rates of the key wastewater nutrients, ammonium and phosphorus by different aquatic macrophytes can be used for optimization of composition of wastewater effluents. The generated data suggest that the composition of the petrochemicals can be modified in a targeted fashion, not only by using different species, but also by changing the source plants' metabolic profile, by exposing them to different abiotic or biotic stresses. This study presents an attractive, ecologically friendly and cost-effective solution for efficient bio-filtration of swine wastewater and petrochemicals production from generated biomass.

  11. Functional Genomic Approaches for the Study of Fetal/Placental Development in Swine with Special Emphasis on Imprinted Genes

    USDA-ARS?s Scientific Manuscript database

    The overall focus of this chapter will be the application of functional genomic approaches for the study of the imprinted gene family in swine. While there are varied definitions of “functional genomics” in general they focus on the application of genomic approaches such as DNA microarrays, single n...

  12. Coupling Cover Crops with Alternative Swine Manure Application Strategies: Manure-15N Tracer Studies

    USDA-ARS?s Scientific Manuscript database

    Integration of rye cover crops with alternative liquid swine (Sus scrofa L.) manure application strategies may enhance retention of manure N in corn (Zea mays L.) - soybean [Glycine max (L.) Merr] cropping systems. The objective of this study was to quantify uptake of manure derived-N by a rye (Seca...

  13. 40 CFR 122.42 - Additional conditions applicable to specified categories of NPDES permits (applicable to State...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...; (vii) Identify protocols for appropriate testing of manure, litter, process wastewater, and soil; (viii... under roof (beef cattle, broilers, layers, swine weighing 55 pounds or more, swine weighing less than 55... paragraph (e)(5)(ii) of this section, the results of any soil testing for nitrogen and phosphorus taken...

  14. 40 CFR 122.42 - Additional conditions applicable to specified categories of NPDES permits (applicable to State...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...; (vii) Identify protocols for appropriate testing of manure, litter, process wastewater, and soil; (viii... under roof (beef cattle, broilers, layers, swine weighing 55 pounds or more, swine weighing less than 55... paragraph (e)(5)(ii) of this section, the results of any soil testing for nitrogen and phosphorus taken...

  15. 40 CFR 122.42 - Additional conditions applicable to specified categories of NPDES permits (applicable to State...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...; (vii) Identify protocols for appropriate testing of manure, litter, process wastewater, and soil; (viii... under roof (beef cattle, broilers, layers, swine weighing 55 pounds or more, swine weighing less than 55... paragraph (e)(5)(ii) of this section, the results of any soil testing for nitrogen and phosphorus taken...

  16. 40 CFR 425.33 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). 425.33 Section 425.33 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  17. 40 CFR 429.23 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). [Reserved] 429.23 Section 429.23 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND...

  18. 40 CFR 429.113 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). 429.113 Section 429.113 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARD...

  19. 40 CFR 429.93 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). 429.93 Section 429.93 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  20. 40 CFR 425.53 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). 425.53 Section 425.53 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  1. 40 CFR 429.33 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). 429.33 Section 429.33 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  2. 40 CFR 429.83 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). [Reserved] 429.83 Section 429.83 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND...

  3. 40 CFR 429.103 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). 429.103 Section 429.103 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARD...

  4. 40 CFR 429.123 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). 429.123 Section 429.123 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARD...

  5. 40 CFR 425.23 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). 425.23 Section 425.23 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  6. 40 CFR 425.83 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). 425.83 Section 425.83 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  7. 40 CFR 425.93 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). 425.93 Section 425.93 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  8. 40 CFR 425.43 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). 425.43 Section 425.43 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  9. 40 CFR 425.13 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). 425.13 Section 425.13 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  10. 40 CFR 425.63 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). 425.63 Section 425.63 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  11. 40 CFR 429.133 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). 429.133 Section 429.133 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARD...

  12. 40 CFR 425.73 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). 425.73 Section 425.73 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  13. 40 CFR 429.43 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations representing the degree of effluent reduction attainable by the application of the best available technology economically achievable (BAT). 429.43 Section 429.43 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  14. 40 CFR 432.13 - Effluent limitations attainable by the application of the best available technology economically...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... application of the best available technology economically achievable (BAT). 432.13 Section 432.13 Protection... limitations attainable by the application of the best available technology economically achievable (BAT... effluent limitations representing the application of BAT: Effluent Limitations [BAT] Regulatedparameter...

  15. 40 CFR 432.13 - Effluent limitations attainable by the application of the best available technology economically...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... application of the best available technology economically achievable (BAT). 432.13 Section 432.13 Protection... limitations attainable by the application of the best available technology economically achievable (BAT... effluent limitations representing the application of BAT: Effluent Limitations [BAT] Regulatedparameter...

  16. 40 CFR 432.113 - Effluent limitations attainable by the application of the best available technology economically...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... application of the best available technology economically achievable (BAT). 432.113 Section 432.113 Protection... limitations attainable by the application of the best available technology economically achievable (BAT... effluent limitations representing the application of BAT: Effluent Limitations [BAT] Regulatedparameter...

  17. 40 CFR 432.113 - Effluent limitations attainable by the application of the best available technology economically...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... application of the best available technology economically achievable (BAT). 432.113 Section 432.113 Protection... limitations attainable by the application of the best available technology economically achievable (BAT... effluent limitations representing the application of BAT: Effluent Limitations [BAT] Regulatedparameter...

  18. 40 CFR 432.13 - Effluent limitations attainable by the application of the best available technology economically...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... application of the best available technology economically achievable (BAT). 432.13 Section 432.13 Protection... limitations attainable by the application of the best available technology economically achievable (BAT... effluent limitations representing the application of BAT: Effluent Limitations [BAT] Regulatedparameter...

  19. 40 CFR 432.113 - Effluent limitations attainable by the application of the best available technology economically...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... application of the best available technology economically achievable (BAT). 432.113 Section 432.113 Protection... limitations attainable by the application of the best available technology economically achievable (BAT... effluent limitations representing the application of BAT: Effluent Limitations [BAT] Regulatedparameter...

  20. Temporal succession of soil antibiotic resistance genes following application of swine, cattle and poultry manures spiked with or without antibiotics.

    PubMed

    Zhang, Yu-Jing; Hu, Hang-Wei; Gou, Min; Wang, Jun-Tao; Chen, Deli; He, Ji-Zheng

    2017-12-01

    Land application of animal manure is a common agricultural practice potentially leading to dispersal and propagation of antibiotic resistance genes (ARGs) in environmental settings. However, the fate of resistome in agro-ecosystems over time following application of different manure sources has never been compared systematically. Here, soil microcosm incubation was conducted to compare effects of poultry, cattle and swine manures spiked with or without the antibiotic tylosin on the temporal changes of soil ARGs. The high-throughput quantitative PCR detected a total of 185 unique ARGs, with Macrolide-Lincosamide-Streptogramin B resistance as the most frequently encountered ARG type. The diversity and abundance of ARGs significantly increased following application of manure and manure spiked with tylosin, with more pronounced effects observed in the swine and poultry manure treatments than in the cattle manure treatment. The level of antibiotic resistance gradually decreased over time in all manured soils but was still significantly higher in the soils treated with swine and poultry manures than in the untreated soils after 130 days' incubation. Tylosin-amended soils consistently showed higher abundances of ARGs than soils treated with manure only, suggesting a strong selection pressure of antibiotic-spiked manure on soil ARGs. The relative abundance of ARGs had significantly positive correlations with integrase and transposase genes, indicative of horizontal transfer potential of ARGs in manure and tylosin treated soils. Our findings provide evidence that application of swine and poultry manures might enrich more soil ARGs than cattle manure, which necessitates the appropriate treatment of raw animal manures prior to land application to minimise the spread of environmental ARGs. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. 40 CFR 445.11 - Effluent limitations attainable by the application of the best practicable control technology...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... application of the best practicable control technology currently available (BPT). 445.11 Section 445.11... attainable by the application of the best practicable control technology currently available (BPT). Except as... the following effluent limitations which represent the application of BPT: Effluent Limitations...

  2. 40 CFR 439.14 - Effluent limitations attainable by the application of best available technology economically...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... application of best available technology economically achievable (BAT). 439.14 Section 439.14 Protection of... by the application of best available technology economically achievable (BAT). (a) Except as provided... following effluent limitations representing the application of BAT: Effluent Limitations (BAT) Regulated...

  3. 40 CFR 439.14 - Effluent limitations attainable by the application of best available technology economically...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... application of best available technology economically achievable (BAT). 439.14 Section 439.14 Protection of... by the application of best available technology economically achievable (BAT). (a) Except as provided... following effluent limitations representing the application of BAT: Effluent Limitations (BAT) Regulated...

  4. 40 CFR 439.14 - Effluent limitations attainable by the application of best available technology economically...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... application of best available technology economically achievable (BAT). 439.14 Section 439.14 Protection of... application of best available technology economically achievable (BAT). (a) Except as provided in 40 CFR 125... effluent limitations representing the application of BAT: Effluent Limitations (BAT) Regulated parameter...

  5. 40 CFR 432.123 - Effluent limitations attainable by the application of the best available technology economically...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... application of the best available technology economically achievable (BAT). 432.123 Section 432.123 Protection... limitations attainable by the application of the best available technology economically achievable (BAT... the following effluent limitations representing the application of BAT: Effluent Limitations [BAT...

  6. 40 CFR 432.103 - Effluent limitations attainable by the application of the best available technology economically...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... application of the best available technology economically achievable (BAT). 432.103 Section 432.103 Protection... attainable by the application of the best available technology economically achievable (BAT). Except as... the following effluent limitations representing the application of BAT: Effluent Limitations [BAT...

  7. 40 CFR 432.123 - Effluent limitations attainable by the application of the best available technology economically...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... application of the best available technology economically achievable (BAT). 432.123 Section 432.123 Protection... limitations attainable by the application of the best available technology economically achievable (BAT... the following effluent limitations representing the application of BAT: Effluent Limitations [BAT...

  8. 40 CFR 432.123 - Effluent limitations attainable by the application of the best available technology economically...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... application of the best available technology economically achievable (BAT). 432.123 Section 432.123 Protection... attainable by the application of the best available technology economically achievable (BAT). Except as... effluent limitations representing the application of BAT: Effluent Limitations [BAT] Regulatedparameter...

  9. 40 CFR 439.14 - Effluent limitations attainable by the application of best available technology economically...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... application of best available technology economically achievable (BAT). 439.14 Section 439.14 Protection of... application of best available technology economically achievable (BAT). (a) Except as provided in 40 CFR 125... effluent limitations representing the application of BAT: Effluent Limitations (BAT) Regulated parameter...

  10. 40 CFR 439.14 - Effluent limitations attainable by the application of best available technology economically...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... application of best available technology economically achievable (BAT). 439.14 Section 439.14 Protection of... by the application of best available technology economically achievable (BAT). (a) Except as provided... following effluent limitations representing the application of BAT: Effluent Limitations (BAT) Regulated...

  11. 40 CFR 432.103 - Effluent limitations attainable by the application of the best available technology economically...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... application of the best available technology economically achievable (BAT). 432.103 Section 432.103 Protection... attainable by the application of the best available technology economically achievable (BAT). Except as... the following effluent limitations representing the application of BAT: Effluent Limitations [BAT...

  12. 40 CFR 432.103 - Effluent limitations attainable by the application of the best available technology economically...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... application of the best available technology economically achievable (BAT). 432.103 Section 432.103 Protection... attainable by the application of the best available technology economically achievable (BAT). Except as... the following effluent limitations representing the application of BAT: Effluent Limitations [BAT...

  13. 40 CFR 432.103 - Effluent limitations attainable by the application of the best available technology economically...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... application of the best available technology economically achievable (BAT). 432.103 Section 432.103 Protection... application of the best available technology economically achievable (BAT). Except as provided by 40 CFR 125... effluent limitations representing the application of BAT: Effluent Limitations [BAT] Regulatedparameter...

  14. 40 CFR 432.123 - Effluent limitations attainable by the application of the best available technology economically...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... application of the best available technology economically achievable (BAT). 432.123 Section 432.123 Protection... attainable by the application of the best available technology economically achievable (BAT). Except as... effluent limitations representing the application of BAT: Effluent Limitations [BAT] Regulatedparameter...

  15. 40 CFR 432.123 - Effluent limitations attainable by the application of the best available technology economically...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... application of the best available technology economically achievable (BAT). 432.123 Section 432.123 Protection... limitations attainable by the application of the best available technology economically achievable (BAT... the following effluent limitations representing the application of BAT: Effluent Limitations [BAT...

  16. 40 CFR 432.103 - Effluent limitations attainable by the application of the best available technology economically...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... application of the best available technology economically achievable (BAT). 432.103 Section 432.103 Protection... application of the best available technology economically achievable (BAT). Except as provided by 40 CFR 125... effluent limitations representing the application of BAT: Effluent Limitations [BAT] Regulatedparameter...

  17. 40 CFR 440.72 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... attainable by the application of the best practicable control technology currently available (BPT). Except as... reduction attainable by the application of the best practicable control technology currently available (BPT...

  18. 40 CFR 440.63 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT): (a) The concentration of...

  19. 40 CFR 440.12 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... by the application of the best practicable control technology currently available (BPT). Except as... reduction attainable after application of the best practicable control technology currently available (BPT...

  20. 40 CFR 440.22 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... application of the best practicable control technology currently available (BPT). Except as provided in... application of the best practicable control technology currently available (BPT). The concentration of...

  1. 40 CFR 440.62 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... attainable by the application of the best practicable control technology currently available (BPT). Except as... reduction attainable by the application of the best practicable control technology currently available (BPT...

  2. 40 CFR 440.62 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... application of the best practicable control technology currently available (BPT). Except as provided in... attainable by the application of the best practicable control technology currently available (BPT): (a) The...

  3. 40 CFR 440.72 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... application of the best practicable control technology currently available (BPT). Except as provided in... attainable by the application of the best practicable control technology currently available (BPT): (a) The...

  4. 40 CFR 440.12 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... by the application of the best practicable control technology currently available (BPT). Except as... reduction attainable after application of the best practicable control technology currently available (BPT...

  5. 40 CFR 440.42 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... attainable by the application of the best practicable control technology currently available (BPT). Except as... reduction attainable after application of the best practicable control technology currently available (BPT...

  6. 40 CFR 440.22 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... attainable by the application of the best practicable control technology currently available (BPT). Except as... attainable by the application of the best practicable control technology currently available (BPT). The...

  7. 40 CFR 440.23 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT). The concentration of pollutants...

  8. 40 CFR 440.72 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... attainable by the application of the best practicable control technology currently available (BPT). Except as... reduction attainable by the application of the best practicable control technology currently available (BPT...

  9. 40 CFR 440.42 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... attainable by the application of the best practicable control technology currently available (BPT). Except as... reduction attainable after application of the best practicable control technology currently available (BPT...

  10. 40 CFR 440.53 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT): (a) The concentration of...

  11. 40 CFR 440.22 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... application of the best practicable control technology currently available (BPT). Except as provided in... application of the best practicable control technology currently available (BPT). The concentration of...

  12. 40 CFR 440.23 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT). The concentration of pollutants...

  13. 40 CFR 440.43 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT). (a) The concentration of...

  14. 40 CFR 440.53 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT): (a) The concentration of...

  15. 40 CFR 440.53 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT): (a) The concentration of...

  16. 40 CFR 440.63 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT): (a) The concentration of...

  17. 40 CFR 440.62 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... application of the best practicable control technology currently available (BPT). Except as provided in... attainable by the application of the best practicable control technology currently available (BPT): (a) The...

  18. 40 CFR 440.43 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT). (a) The concentration of...

  19. 40 CFR 440.42 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... application of the best practicable control technology currently available (BPT). Except as provided in... attainable after application of the best practicable control technology currently available (BPT): (a) The...

  20. 40 CFR 440.63 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT): (a) The concentration of...

  1. 40 CFR 440.62 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... attainable by the application of the best practicable control technology currently available (BPT). Except as... reduction attainable by the application of the best practicable control technology currently available (BPT...

  2. 40 CFR 440.12 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... by the application of the best practicable control technology currently available (BPT). Except as... reduction attainable after application of the best practicable control technology currently available (BPT...

  3. 40 CFR 440.22 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... attainable by the application of the best practicable control technology currently available (BPT). Except as... attainable by the application of the best practicable control technology currently available (BPT). The...

  4. 40 CFR 440.113 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... attainable by the application of the best available technology economically achievable (BAT). Except as... reduction attainable by the application of the best available technology economically achievable (BAT): (a...

  5. 40 CFR 440.72 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... application of the best practicable control technology currently available (BPT). Except as provided in... attainable by the application of the best practicable control technology currently available (BPT): (a) The...

  6. 40 CFR 440.13 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT): (a) The concentration of...

  7. 40 CFR 440.72 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... attainable by the application of the best practicable control technology currently available (BPT). Except as... reduction attainable by the application of the best practicable control technology currently available (BPT...

  8. 40 CFR 440.42 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... attainable by the application of the best practicable control technology currently available (BPT). Except as... reduction attainable after application of the best practicable control technology currently available (BPT...

  9. 40 CFR 440.23 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT). The concentration of pollutants...

  10. 40 CFR 440.113 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT): (a) The concentration of...

  11. 40 CFR 440.113 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT): (a) The concentration of...

  12. 40 CFR 440.23 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT). The concentration of pollutants...

  13. 40 CFR 440.43 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT). (a) The concentration of...

  14. 40 CFR 440.62 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... attainable by the application of the best practicable control technology currently available (BPT). Except as... reduction attainable by the application of the best practicable control technology currently available (BPT...

  15. 40 CFR 440.113 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... attainable by the application of the best available technology economically achievable (BAT). Except as... reduction attainable by the application of the best available technology economically achievable (BAT): (a...

  16. 40 CFR 440.12 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... application of the best practicable control technology currently available (BPT). Except as provided in... attainable after application of the best practicable control technology currently available (BPT): (a) The...

  17. 40 CFR 440.13 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT): (a) The concentration of...

  18. 40 CFR 440.22 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... attainable by the application of the best practicable control technology currently available (BPT). Except as... attainable by the application of the best practicable control technology currently available (BPT). The...

  19. 40 CFR 440.63 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT): (a) The concentration of...

  20. 40 CFR 440.53 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT): (a) The concentration of...

  1. Effect of manure application rate and rainfall timing on the leaching of antibiotic-resistant bacteria and their associated genes

    USDA-ARS?s Scientific Manuscript database

    In this study we investigate the effect of application rate and timing of liquid swine slurry on leaching of antibiotic-resistant bacteria (ARB) and their antibiotic-resistance genes (ARG) through soil columns. Swine slurry was added to laboratory soil columns at rates of 5,000 or 30,000 gallons acr...

  2. 40 CFR 427.53 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ASBESTOS MANUFACTURING POINT SOURCE CATEGORY Asbestos Millboard... by the application of the best available technology economically achievable. The following...

  3. 40 CFR 411.13 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CEMENT MANUFACTURING POINT SOURCE CATEGORY Nonleaching... by the application of the best available technology economically achievable. The following...

  4. 40 CFR 427.103 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ASBESTOS MANUFACTURING POINT SOURCE CATEGORY Vapor Absorption... by the application of the best available technology economically achievable. The following...

  5. 40 CFR 427.63 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ASBESTOS MANUFACTURING POINT SOURCE CATEGORY Asbestos Roofing... by the application of the best available technology economically achievable. The following...

  6. 40 CFR 415.432 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... technology currently available (BPT). 415.432 Section 415.432 Protection of Environment ENVIRONMENTAL... degree of effluent reduction attainable by the application of the best practicable control technology... effluent reduction attainable by the application of the best practicable control technology currently...

  7. 40 CFR 415.272 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... technology currently available (BPT). 415.272 Section 415.272 Protection of Environment ENVIRONMENTAL... degree of effluent reduction attainable by the application of the best practicable control technology... effluent reduction attainable by the application of the best practicable control technology currently...

  8. 40 CFR 415.292 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... technology currently available (BPT). 415.292 Section 415.292 Protection of Environment ENVIRONMENTAL... degree of effluent reduction attainable by the application of the best practicable control technology... effluent reduction attainable by the application of the best practicable control technology currently...

  9. 40 CFR 429.53 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... best available technology economically achievable (BAT): There shall be no discharge of process... degree of effluent reduction attainable by the application of the best available technology economically...) EFFLUENT GUIDELINES AND STANDARDS TIMBER PRODUCTS PROCESSING POINT SOURCE CATEGORY Dry Process Hardboard...

  10. 40 CFR 430.34 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... biocides are used must achieve the following effluent limitations representing the degree of effluent... shall be subject to concentration limitations. Concentration limitations are only applicable to non-continuous dischargers. Permittees not using chlorophenolic-containing biocides must certify to the permit...

  11. 40 CFR 430.14 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... biocides are used must achieve the following effluent limitations representing the degree of effluent... shall be subject to concentration limitations. Concentration limitations are only applicable to non-continuous dischargers. Permittees not using chlorophenolic-containing biocides must certify to the permit...

  12. 40 CFR 427.73 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ASBESTOS MANUFACTURING POINT SOURCE CATEGORY Asbestos Floor... attainable by the application of the best available technology economically achievable. The following...

  13. 40 CFR 411.23 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CEMENT MANUFACTURING POINT SOURCE CATEGORY Leaching Subcategory... application of the best available technology economically achievable. The following limitations establish the...

  14. 40 CFR 430.104 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... chlorophenolic-containing biocides are used must achieve the following effluent limitations representing the... applicable to non-continuous dischargers. Permittees not using chlorophenolic-containing biocides must certify to the permit-issuing authority that they are not using these biocides: Subpart J [BAT effluent...

  15. 40 CFR 429.171 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TIMBER PRODUCTS PROCESSING POINT SOURCE CATEGORY Wood Furniture... best practicable control technology currently available (BPT). Except as provided in 40 CFR 125.30...

  16. 40 CFR 429.173 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TIMBER PRODUCTS PROCESSING POINT SOURCE CATEGORY Wood Furniture... best available technology economically achievable (BAT). Except as provided in 40 CFR 125.30 through...

  17. 40 CFR 463.32 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS PLASTICS MOLDING AND FORMING POINT SOURCE CATEGORY... reduction attainable by the application of the best practicable control technology currently available...

  18. 40 CFR 463.22 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best practicable control... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS PLASTICS MOLDING AND FORMING POINT SOURCE CATEGORY... reduction attainable by the application of the best practicable control technology currently available...

  19. 40 CFR 445.13 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... limitations which represent the application of BAT: Limitations for ammonia (as N), a-terpineol, aniline... degree of effluent reduction attainable by the application of best available technology economically... application of best available technology economically achievable (BAT). Except as provided in 40 CFR 125.30...

  20. 40 CFR 467.43 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically achievable. [Reserved] 467.43 Section 467.43 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... application of the best available technology economically achievable. [Reserved] ...

  1. 40 CFR 467.42 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... degree of effluent reduction attainable by the application of the best practicable control technology currently available. [Reserved] 467.42 Section 467.42 Protection of Environment ENVIRONMENTAL PROTECTION... application of the best practicable control technology currently available. [Reserved] ...

  2. 40 CFR 430.72 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... through the application of the thermo-mechanical process] Pollutant or pollutant property Kg/kkg (or... where pulp and paper at groundwood chemi-mechanical mills are produced] Pollutant or pollutant property... Mechanical Pulp Subcategory § 430.72 Effluent limitations representing the degree of effluent reduction...

  3. 40 CFR 426.112 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... technology currently available (BPT). (The fluoride and lead limitations are applicable to the abrasive polishing and acid polishing waste water streams while the TSS, oil, and pH limitations are applicable to the entire process waste water stream): Effluent characteristic Effluent limitations Maximum for any 1...

  4. 40 CFR 426.112 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... technology currently available (BPT). (The fluoride and lead limitations are applicable to the abrasive polishing and acid polishing waste water streams while the TSS, oil, and pH limitations are applicable to the entire process waste water stream): Effluent characteristic Effluent limitations Maximum for any 1...

  5. 40 CFR 426.112 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... technology currently available (BPT). (The fluoride and lead limitations are applicable to the abrasive polishing and acid polishing waste water streams while the TSS, oil, and pH limitations are applicable to the entire process waste water stream): Effluent characteristic Effluent limitations Maximum for any 1...

  6. Field test of methane fermentation system for treating swine wastes.

    PubMed

    Kataoka, N; Suzuki, T; Ishida, K; Yamada, N; Kurata, N; Katayose, M; Honda, K

    2002-01-01

    A methane fermentation system for treating swine wastes was developed and successfully demonstrated in a field test plant (0.5 m3/d). The system was composed of a screw-press dehydrator, a methanogenic digester, a sludge separator, an oxidation ditch (OD) and composting equipment. A performance evaluation was carried out regarding physical pre-treatment using the screw-press dehydrator, methane fermentation for pre-treated slurry, and post-treatment for digested effluent by OD. Total solids (TS) and chemical oxygen demand (CODCr) removal by the screw-press pre-treatment were 38% and 22%, respectively. Properties of the screenings were as follows: water content 57%, ignition loss 93%, specific gravity 0.33. The pretreated strong slurry was digested under mesophilic conditions. Digestion gas (biogas) production rate was 25 m3/m3-slurry (NTP) and methane content of the biogas was 67%. CODCr removal of 65% with methane fermentation treatment of the slurry operating at 35 degrees C was observed. No inhibition of methane fermentation reaction occurred at the NH4(+)-N concentration of 3,000 mg/l or less during methane fermentation by the system. Mass balance from the present pilot-scale study showed that 1 m3 of mixture of excrement and urine of swine waste (TS 90 kg/m3) was biologically converted to 25 m3/m3-slurry (NTP) of biogas (methane content 67%), 100 kg of compost (water content 40%, ignition loss 75%), and 0.80 m3 of treated water (SS 30-70 mg/l).

  7. Effects of spray-irrigated treated effluent on water quantity and quality, and the fate and transport of nitrogen in a small watershed, New Garden Township, Chester County, Pennsylvania

    USGS Publications Warehouse

    Schreffler, Curtis L.; Galeone, Daniel G.; Veneziale, John M.; Olson, Leif E.; O'Brien, David L.

    2005-01-01

    An increasing number of communities in Pennsylvania are implementing land-treatment systems to dispose of treated sewage effluent. Disposal of treated effluent by spraying onto the land surface, instead of discharging to streams, may recharge the ground-water system and reduce degradation of stream-water quality. The U.S. Geological Survey (USGS), in cooperation with the Pennsylvania Department of Environmental Protection (PaDEP) and the Chester County Water Resources Authority (CCWRA) and with assistance from the New Garden Township Sewer Authority, conducted a study from October 1997 through December 2001 to assess the effects of spray irrigation of secondary treated sewage effluent on the water quantity and quality and the fate and transport of nitrogen in a 38-acre watershed in New Garden Township, Chester County, Pa. On an annual basis, the spray irrigation increased the recharge to the watershed. Compared to the annual recharge determined for the Red Clay Creek watershed above the USGS streamflow-gaging station (01479820) near Kennett Square, Pa., the spray irrigation increased annual recharge in the study watershed by approximately 8.8 in. (inches) in 2000 and 4.3 in. in 2001. For 2000 and 2001, the spray irrigation increased recharge 65-70 percent more than the recharge estimates determined for the Red Clay Creek watershed. The increased recharge was equal to 30-39 percent of the applied effluent. The spray-irrigated effluent increased base flow in the watershed. The magnitude of the increase appeared to be related to the time of year when the application rates increased. During the late fall through winter and into the early spring period, when application rates were low, base flow increased by approximately 50 percent over the period prior to effluent application. During the early spring through summer to the late fall period, when application rates were high, base flow increased by approximately 200 percent over the period prior to effluent application. The spray-irrigated effluent affected the ground-water quality of the shallow aquifer differently on the hilltop and hillside topographic settings of the watershed where spray irrigation was being applied (application area). Concentrations of nitrate-nitrogen (nitrate N) and chloride (Cl) in the effluent were higher than concentrations of these constituents in shallow ground water from wells on the hilltop and hillside prior to start of spray irrigation. In water from wells on the hilltop, concentrations of nitrate N and Cl increased in samples collected during effluent application compared to samples collected prior to effluent application. Also, increasing trends in concentration of these two constituents were evident through the study period. In water from wells on the hillside, which were on the eastern part of the application area, nitrate N and Cl concentrations increased in samples collected during effluent application compared to samples collected prior to effluent application. Also, increasing trends in concentration of these two constituents were evident through the study period. However, on the hillside of the western application area, the ground-water quality was not affected by the spray-irrigated effluent because of the greater thickness of unconsolidated material and higher amounts of clay present in those unconsolidated sands. Although nitrate N concentrations increased in water from hilltop and hillside wells in the application area, the nitrate N concentrations were below the effluent concentration. A combination of plant uptake, biological activity, and denitrification may be the processes accounting for the lower nitrate N concentrations in shallow ground water compared to the spray-irrigated effluent. Cl concentrations in water from hilltop western application area well Ch-5173 increased during the study period but were an order of magnitude less than the input effluent concentration. Cl concentrations in shallow ground water in the e

  8. 40 CFR 420.43 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... best available technology economically achievable. (a) Electric arc furnace steelmaking—semi-wet. No... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in 40 CFR 125...

  9. 40 CFR 420.42 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... best practicable control technology currently available. (a) Electric arc furnace steelmaking—semi-wet... degree of effluent reduction attainable by the application of the best practicable control technology... application of the best practicable control technology currently available (BPT). Except as provided in 40 CFR...

  10. 40 CFR 445.23 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... achievable (BAT). 445.23 Section 445.23 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... the application of the best available technology economically achievable (BAT). Except as provided in... effluent limitations which represent the application of BAT: Limitations for ammonia (as N), a-terpineol...

  11. 40 CFR 425.23 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... achievable (BAT). 425.23 Section 425.23 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... attainable by the application of the best available technology economically achievable (BAT). Except as... application of the best available technology economically achievable (BAT): The effluent limitations are those...

  12. 40 CFR 430.24 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... achievable (BAT). 430.24 Section 430.24 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... effluent reduction attainable by the application of best available technology economically achievable (BAT... attainable by the application of the best available technology economically achievable (BAT). (a) Except as...

  13. 40 CFR 425.43 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... achievable (BAT). 425.43 Section 425.43 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... attainable by the application of the best available technology economically achievable (BAT). Except as... application of the best available technology economically achievable (BAT): The effluent limitations are those...

  14. 40 CFR 425.93 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... achievable (BAT). 425.93 Section 425.93 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... attainable by the application of the best available technology economically achievable (BAT). Except as... application of the best available technology economically achievable (BAT): The effluent limitations are those...

  15. 40 CFR 425.43 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... achievable (BAT). 425.43 Section 425.43 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... attainable by the application of the best available technology economically achievable (BAT). Except as... application of the best available technology economically achievable (BAT): The effluent limitations are those...

  16. 40 CFR 445.13 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... achievable (BAT). 445.13 Section 445.13 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... the application of best available technology economically achievable (BAT). Except as provided in 40... following effluent limitations which represent the application of BAT: Limitations for ammonia (as N), a...

  17. 40 CFR 425.13 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... achievable (BAT). 425.13 Section 425.13 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... attainable by the application of the best available technology economically achievable (BAT). Except as... application of the best available technology economically achievable (BAT): The effluent limitations are those...

  18. 40 CFR 425.63 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... achievable (BAT). 425.63 Section 425.63 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... attainable by the application of the best available technology economically achievable (BAT). Except as... application of the best available technology economically achievable (BAT): The effluent limitations are those...

  19. 40 CFR 425.63 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... achievable (BAT). 425.63 Section 425.63 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... attainable by the application of the best available technology economically achievable (BAT). Except as... application of the best available technology economically achievable (BAT): The effluent limitations are those...

  20. 40 CFR 425.93 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... achievable (BAT). 425.93 Section 425.93 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... attainable by the application of the best available technology economically achievable (BAT). Except as... application of the best available technology economically achievable (BAT): The effluent limitations are those...

  1. 40 CFR 430.24 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... achievable (BAT). 430.24 Section 430.24 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... effluent reduction attainable by the application of best available technology economically achievable (BAT... attainable by the application of the best available technology economically achievable (BAT). (a) Except as...

  2. 40 CFR 418.63 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... application of the best available technology economically achievable: There shall be no discharge of process... representing the degree of effluent reduction attainable by the application of the best available technology... attainable by the application of the best available technology economically achievable. The following...

  3. 40 CFR 406.73 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... application of the best available technology economically achievable. The following limitations establish the... subject to the provisions of this subpart after application of the best available technology economically...

  4. 40 CFR 406.83 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... application of the best available technology economically achievable. The following limitations establish the... subject to the provisions of this subpart after application of the best available technology economically...

  5. 40 CFR 434.74 - Effluent limitations attainable by application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... application of the best conventional pollutant control technology (BCT). 434.74 Section 434.74 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS COAL MINING POINT... Effluent limitations attainable by application of the best conventional pollutant control technology (BCT...

  6. 40 CFR 440.13 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best available technology economically... of the best available technology economically achievable (BAT). Except as provided in subpart L of... application of the best available technology economically achievable (BAT): (a) The concentration of...

  7. 40 CFR 469.29 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... degree of effluent reduction attainable by the application of the best conventional pollution control... by the application of the best conventional pollution control technology (BCT). Except as provided in... of the best conventional pollution control technology (BCT): Subpart B—Electronic Crystals BCT...

  8. 40 CFR 420.42 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... best practicable control technology currently available. (a) Electric arc furnace steelmaking—semi-wet... degree of effluent reduction attainable by the application of the best practicable control technology... application of the best practicable control technology currently available (BPT). Except as provided in 40 CFR...

  9. 40 CFR 420.42 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... best practicable control technology currently available. (a) Electric arc furnace steelmaking—semi-wet... degree of effluent reduction attainable by the application of the best practicable control technology... application of the best practicable control technology currently available (BPT). Except as provided in 40 CFR...

  10. 40 CFR 420.42 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... best practicable control technology currently available. (a) Electric arc furnace steelmaking—semi-wet... degree of effluent reduction attainable by the application of the best practicable control technology... application of the best practicable control technology currently available (BPT). Except as provided in 40 CFR...

  11. 40 CFR 420.42 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... best practicable control technology currently available. (a) Electric arc furnace steelmaking—semi-wet... degree of effluent reduction attainable by the application of the best practicable control technology... application of the best practicable control technology currently available (BPT). Except as provided in 40 CFR...

  12. 40 CFR 420.43 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... best available technology economically achievable. (a) Electric arc furnace steelmaking—semi-wet. No... degree of effluent reduction attainable by the application of the best available technology economically... application of the best available technology economically achievable (BAT). Except as provided in 40 CFR 125...

  13. 40 CFR 429.143 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TIMBER PRODUCTS PROCESSING POINT SOURCE CATEGORY Particleboard Manufacturing Subcategory § 429.143 Effluent limitations representing the degree of effluent reduction...

  14. 40 CFR 429.142 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TIMBER PRODUCTS PROCESSING POINT SOURCE CATEGORY Particleboard Manufacturing Subcategory § 429.142 Effluent limitations representing the degree of effluent...

  15. 40 CFR 429.141 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TIMBER PRODUCTS PROCESSING POINT SOURCE CATEGORY Particleboard Manufacturing Subcategory § 429.141 Effluent limitations representing the degree of effluent reduction...

  16. Serologic test development. Progress report, July 1, 1974--June 30, 1975. [Radioimmunoassay method for diagnosis of swine and bovine diseases

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Saunders, G.C.; Clinard, E.H.; Sanders, W.M.

    1975-01-01

    The Enzyme-Labeled Antibody (ELA) test system has been adapted to microtiter trays for both cell bound and soluble antigens. Problems involving both readout instrumentation and reaction product stability have been solved. Progress involving application of the ELA system for detection of hog cholera, trichinosis, swine brucellosis, and swine and bovine tuberculosis is reported. Prototype instrumentation for automating ELA processing is being developed. (auth)

  17. 40 CFR 430.84 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... achievable (BAT). [Reserved] 430.84 Section 430.84 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... effluent reduction attainable by the application of best available technology economically achievable (BAT...

  18. 40 CFR 430.84 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... achievable (BAT). [Reserved] 430.84 Section 430.84 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... effluent reduction attainable by the application of best available technology economically achievable (BAT...

  19. Molecular Diversity of Bacteroidales in Fecal and Environmental Samples and Swine-Associated Subpopulations

    PubMed Central

    Lamendella, Regina; Li, Kent C.; Oerther, Daniel

    2013-01-01

    Several swine-specific microbial source tracking methods are based on PCR assays targeting Bacteroidales 16S rRNA gene sequences. The limited application of these assays can be explained by the poor understanding of their molecular diversity in fecal sources and environmental waters. In order to address this, we studied the diversity of 9,340 partial (>600 bp in length) Bacteroidales 16S rRNA gene sequences from 13 fecal sources and nine feces-contaminated watersheds. The compositions of major Bacteroidales populations were analyzed to determine which host and environmental sequences were contributing to each group. This information allowed us to identify populations which were both exclusive to swine fecal sources and detected in swine-contaminated waters. Phylogenetic and diversity analyses revealed that some markers previously believed to be highly specific to swine populations are shared by multiple hosts, potentially explaining the cross-amplification signals obtained with nontargeted hosts. These data suggest that while many Bacteroidales populations are cosmopolitan, others exhibit a preferential host distribution and may be able to survive different environmental conditions. This study further demonstrates the importance of elucidating the diversity patterns of targeted bacterial groups to develop more inclusive fecal source tracking applications. PMID:23160126

  20. Spread of multidrug-resistant Escherichia coli harboring integron via swine farm waste water treatment plant.

    PubMed

    Park, Jin-Hyeong; Kim, Young-Ji; Binn-Kim; Seo, Kun-Ho

    2018-03-01

    Wastewater treatment plants (WWTPs) that release treated wastewater into the environment have emerged as a major threat to public health. In this study, we investigated Escherichia coli load and antibiotic-resistance profiles across different treatment processes at a swine farm WWTP. The frequency of the detection of class 1 and 2 integrons, and their association with antibiotic resistance, were also analyzed. Samples were obtained at each of five sampling sites that represented each processing step within the WWTP. The largest decrease in E. coli load was observed during the anaerobic digestion step (from 4.86 to 2.89log CFU/mL). Isolates resistant to β-lactam antibiotics were efficiently removed after a series of treatment steps, whereas the proportions of isolates resistant to non-β-lactam antibiotics and multidrug-resistant strains were maintained across treatments. The occurrence of integron-positive strains was not significantly different at the various sampling sites (43.4-70%; p>0.05). Of the class 1 integron-positive isolates, 17.9% harbored the integron-associated gene cassettes aadA2, aadA12, aadA22, and dfrA15. To the best of our knowledge, this is the first description of a class 1 integron containing the aadA12 gene cassette from a swine farm and the presence of a class 1 integron containing dfrA15 in E. coli. This suggests that novel antibiotic-resistance gene cassette arrays could be generated in swine farm WWTPs. Moreover, 75% of integron-positive strains were categorized as multidrug resistant, whereas only 15.4% of integron-negative strains were multidrug resistant (p<0.05), indicating that integrons may be responsible for mediating resistance in WWTPs. With regard to the occurrence of multidrug-resistant, integron-positive E. coli recovered from the final effluent, our results highlighted the potential risks associated with wastewater discharge from swine farm WWTPs in terms of the spread of antibiotic-resistant bacteria to the aquatic environment. Copyright © 2017 Elsevier Inc. All rights reserved.

  1. Greenhouse gas emission from the total process of swine manure composting and land application of compost

    NASA Astrophysics Data System (ADS)

    Zhong, Jia; Wei, Yuansong; Wan, Hefeng; Wu, Yulong; Zheng, Jiaxi; Han, Shenghui; Zheng, Bofu

    2013-12-01

    Greenhouse gas (GHG) emissions from animal manure management are of great concern in China. However, there are still great uncertainties about China's GHG inventory due to the GHG emission factors partly used default values from the Intergovernmental Panel of Climate Change (IPCC) guidelines. The purpose of this study was to use a case study in Beijing to determine the regional GHG emission factors based on the combination of swine manure composting and land application of the compost with both on-site examination and a life cycle assessment (LCA). The results showed that the total GHG emission factor was 240 kgCO2eq tDS-1 (dry solids), including the direct GHG emission factor of 115 kgCO2eq tDS-1 for swine manure composting and 48 kgCO2eq tDS-1 for land application of the compost. Among the total GHG emissions of 5.06 kgCH4 tDS-1 and 0.13 kgN2O tDS-1, the swine manure composting contributed approximately 89% to CH4 emissions while land application accounted for 92% of N2O emission. Meanwhile, the GHG emission profile from the full process in Beijing in 2015 and 2020 was predicted by the scenario analysis. The composting and land application is a cost-effective way for animal manure management in China considering GHG emissions.

  2. 40 CFR 429.73 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...) EFFLUENT GUIDELINES AND STANDARDS TIMBER PRODUCTS PROCESSING POINT SOURCE CATEGORY Wood Preserving-Water Borne or Nonpressure Subcategory § 429.73 Effluent limitations representing the degree of effluent...

  3. 40 CFR 429.73 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) EFFLUENT GUIDELINES AND STANDARDS TIMBER PRODUCTS PROCESSING POINT SOURCE CATEGORY Wood Preserving-Water Borne or Nonpressure Subcategory § 429.73 Effluent limitations representing the degree of effluent...

  4. 40 CFR 471.66 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS NONFERROUS METALS FORMING AND METAL POWDERS POINT SOURCE CATEGORY Titanium Forming Subcategory § 471.66 Effluent limitations representing the degree of effluent...

  5. 40 CFR 471.66 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS NONFERROUS METALS FORMING AND METAL POWDERS POINT SOURCE CATEGORY Titanium Forming Subcategory § 471.66 Effluent limitations representing the degree of effluent...

  6. 40 CFR 414.83 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Specialty Organic Chemicals § 414.83 Effluent limitations representing the degree of effluent reduction attainable by the...

  7. 40 CFR 414.82 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Specialty Organic Chemicals § 414.82 Effluent limitations representing the degree of effluent reduction attainable...

  8. 40 CFR 414.63 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Commodity Organic Chemicals § 414.63 Effluent limitations representing the degree of effluent reduction attainable by the...

  9. 40 CFR 414.62 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Commodity Organic Chemicals § 414.62 Effluent limitations representing the degree of effluent reduction attainable...

  10. 40 CFR 414.81 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Specialty Organic Chemicals § 414.81 Effluent limitations representing the degree of effluent reduction attainable...

  11. 40 CFR 414.81 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Specialty Organic Chemicals § 414.81 Effluent limitations representing the degree of effluent reduction attainable...

  12. 40 CFR 414.81 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Specialty Organic Chemicals § 414.81 Effluent limitations representing the degree of effluent reduction attainable...

  13. 40 CFR 414.62 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Commodity Organic Chemicals § 414.62 Effluent limitations representing the degree of effluent reduction attainable...

  14. 40 CFR 414.62 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Commodity Organic Chemicals § 414.62 Effluent limitations representing the degree of effluent reduction attainable...

  15. 40 CFR 414.61 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Commodity Organic Chemicals § 414.61 Effluent limitations representing the degree of effluent reduction attainable...

  16. 40 CFR 414.62 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Commodity Organic Chemicals § 414.62 Effluent limitations representing the degree of effluent reduction attainable...

  17. 40 CFR 414.82 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Specialty Organic Chemicals § 414.82 Effluent limitations representing the degree of effluent reduction attainable...

  18. 40 CFR 414.82 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Specialty Organic Chemicals § 414.82 Effluent limitations representing the degree of effluent reduction attainable...

  19. 40 CFR 414.82 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Specialty Organic Chemicals § 414.82 Effluent limitations representing the degree of effluent reduction attainable...

  20. 40 CFR 414.61 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Commodity Organic Chemicals § 414.61 Effluent limitations representing the degree of effluent reduction attainable...

  1. 40 CFR 414.82 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Specialty Organic Chemicals § 414.82 Effluent limitations representing the degree of effluent reduction attainable...

  2. 40 CFR 414.61 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Commodity Organic Chemicals § 414.61 Effluent limitations representing the degree of effluent reduction attainable...

  3. 40 CFR 414.62 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Commodity Organic Chemicals § 414.62 Effluent limitations representing the degree of effluent reduction attainable...

  4. 40 CFR 414.61 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Commodity Organic Chemicals § 414.61 Effluent limitations representing the degree of effluent reduction attainable...

  5. 40 CFR 414.81 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Specialty Organic Chemicals § 414.81 Effluent limitations representing the degree of effluent reduction attainable...

  6. 40 CFR 414.61 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Commodity Organic Chemicals § 414.61 Effluent limitations representing the degree of effluent reduction attainable...

  7. 40 CFR 414.81 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Specialty Organic Chemicals § 414.81 Effluent limitations representing the degree of effluent reduction attainable...

  8. 40 CFR 440.93 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Antimony Ore Subcategory § 440.93 Effluent limitations representing the degree of effluent reduction...

  9. 40 CFR 440.73 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Nickel Ore Subcategory § 440.73 Effluent limitations representing the degree of effluent reduction...

  10. 40 CFR 440.25 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Aluminum Ore Subcategory § 440.25 Effluent limitations representing the degree of effluent reduction...

  11. 40 CFR 440.45 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Mercury Ore Subcategory § 440.45 Effluent limitations representing the degree of effluent reduction...

  12. 40 CFR 440.55 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Titanium Ore Subcategory § 440.55 Effluent limitations representing the degree of effluent reduction...

  13. 40 CFR 440.95 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Antimony Ore Subcategory § 440.95 Effluent limitations representing the degree of effluent reduction...

  14. 40 CFR 440.65 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Tungsten Ore Subcategory § 440.65 Effluent limitations representing the degree of effluent reduction...

  15. 40 CFR 440.45 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Mercury Ore Subcategory § 440.45 Effluent limitations representing the degree of effluent reduction...

  16. 40 CFR 440.45 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Mercury Ore Subcategory § 440.45 Effluent limitations representing the degree of effluent reduction...

  17. 40 CFR 440.93 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Antimony Ore Subcategory § 440.93 Effluent limitations representing the degree of effluent reduction...

  18. 40 CFR 440.73 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Nickel Ore Subcategory § 440.73 Effluent limitations representing the degree of effluent reduction...

  19. 40 CFR 440.65 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Tungsten Ore Subcategory § 440.65 Effluent limitations representing the degree of effluent reduction...

  20. 40 CFR 440.55 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Titanium Ore Subcategory § 440.55 Effluent limitations representing the degree of effluent reduction...

  1. 40 CFR 440.65 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Tungsten Ore Subcategory § 440.65 Effluent limitations representing the degree of effluent reduction...

  2. 40 CFR 440.25 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Aluminum Ore Subcategory § 440.25 Effluent limitations representing the degree of effluent reduction...

  3. 40 CFR 440.75 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Nickel Ore Subcategory § 440.75 Effluent limitations representing the degree of effluent reduction...

  4. 40 CFR 440.55 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Titanium Ore Subcategory § 440.55 Effluent limitations representing the degree of effluent reduction...

  5. 40 CFR 440.73 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Nickel Ore Subcategory § 440.73 Effluent limitations representing the degree of effluent reduction...

  6. 40 CFR 440.93 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Antimony Ore Subcategory § 440.93 Effluent limitations representing the degree of effluent reduction...

  7. 40 CFR 440.25 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Aluminum Ore Subcategory § 440.25 Effluent limitations representing the degree of effluent reduction...

  8. 40 CFR 440.95 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Antimony Ore Subcategory § 440.95 Effluent limitations representing the degree of effluent reduction...

  9. 40 CFR 440.95 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Antimony Ore Subcategory § 440.95 Effluent limitations representing the degree of effluent reduction...

  10. 40 CFR 440.75 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Nickel Ore Subcategory § 440.75 Effluent limitations representing the degree of effluent reduction...

  11. 40 CFR 440.75 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Nickel Ore Subcategory § 440.75 Effluent limitations representing the degree of effluent reduction...

  12. 40 CFR 414.41 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Thermoplastic... concentration listed in the following table. Effluent characteristics BPT Effluent Limitations 1 Maximum for any...

  13. 40 CFR 414.51 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Thermosetting... concentration listed in the following table. Effluent characteristics BPT effluent limitations 1 Maximum for any...

  14. 40 CFR 445.23 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) EFFLUENT GUIDELINES AND STANDARDS LANDFILLS POINT SOURCE CATEGORY RCRA Subtitle D Non-Hazardous Waste Landfill § 445.23 Effluent limitations representing the degree of effluent reduction attainable by the...

  15. 40 CFR 414.71 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Bulk Organic Chemicals § 414.71 Effluent limitations representing the degree of effluent reduction attainable by the...

  16. 40 CFR 414.72 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Bulk Organic Chemicals § 414.72 Effluent limitations representing the degree of effluent reduction attainable by the...

  17. 40 CFR 414.71 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Bulk Organic Chemicals § 414.71 Effluent limitations representing the degree of effluent reduction attainable by the...

  18. 40 CFR 414.72 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Bulk Organic Chemicals § 414.72 Effluent limitations representing the degree of effluent reduction attainable by the...

  19. 40 CFR 414.72 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Bulk Organic Chemicals § 414.72 Effluent limitations representing the degree of effluent reduction attainable by the...

  20. 40 CFR 414.71 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Bulk Organic Chemicals § 414.71 Effluent limitations representing the degree of effluent reduction attainable by the...

  1. 40 CFR 414.71 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Bulk Organic Chemicals § 414.71 Effluent limitations representing the degree of effluent reduction attainable by the...

  2. 40 CFR 414.71 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Bulk Organic Chemicals § 414.71 Effluent limitations representing the degree of effluent reduction attainable by the...

  3. 40 CFR 414.72 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Bulk Organic Chemicals § 414.72 Effluent limitations representing the degree of effluent reduction attainable by the...

  4. 40 CFR 414.72 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Bulk Organic Chemicals § 414.72 Effluent limitations representing the degree of effluent reduction attainable by the...

  5. 40 CFR 440.112 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORE MINING AND DRESSING POINT SOURCE CATEGORY Platinum Ores Subcategory § 440.112 Effluent limitations representing the degree of effluent reduction...

  6. 40 CFR 440.112 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORE MINING AND DRESSING POINT SOURCE CATEGORY Platinum Ores Subcategory § 440.112 Effluent limitations representing the degree of effluent reduction...

  7. 40 CFR 440.15 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Iron Ore Subcategory § 440.15 Effluent limitations representing the degree of effluent reduction attainable...

  8. 40 CFR 440.92 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORE MINING AND DRESSING POINT SOURCE CATEGORY Antimony Ore Subcategory § 440.92 Effluent limitations representing the degree of effluent reduction...

  9. 40 CFR 440.15 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Iron Ore Subcategory § 440.15 Effluent limitations representing the degree of effluent reduction attainable...

  10. 40 CFR 440.15 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Iron Ore Subcategory § 440.15 Effluent limitations representing the degree of effluent reduction attainable...

  11. 40 CFR 440.92 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORE MINING AND DRESSING POINT SOURCE CATEGORY Antimony Ore Subcategory § 440.92 Effluent limitations representing the degree of effluent reduction...

  12. 40 CFR 440.35 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORE MINING AND DRESSING POINT SOURCE CATEGORY Uranium, Radium and Vanadium Ores Subcategory § 440.35 Effluent limitations representing the degree of effluent...

  13. 40 CFR 440.35 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORE MINING AND DRESSING POINT SOURCE CATEGORY Uranium, Radium and Vanadium Ores Subcategory § 440.35 Effluent limitations representing the degree of effluent...

  14. 40 CFR 414.31 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Other Fibers... concentration listed in the following table. Effluent characteristics BPT effluent limitations 1 Maximum for any...

  15. 40 CFR 414.21 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORGANIC CHEMICALS, PLASTICS, AND SYNTHETIC FIBERS Rayon Fibers... concentration listed in the following table. Effluent characteristics BPT effluent limitations 1 Maximum for any...

  16. 7 CFR 59.200 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... these same swine. Average lean percentage. The term “average lean percentage” means the value equal to the average percentage of the carcass weight comprised of lean meat for the swine slaughtered during the applicable reporting period. Whenever the packer changes the manner in which the average lean...

  17. 7 CFR 59.200 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... these same swine. Average lean percentage. The term “average lean percentage” means the value equal to the average percentage of the carcass weight comprised of lean meat for the swine slaughtered during the applicable reporting period. Whenever the packer changes the manner in which the average lean...

  18. Hanford Facility dangerous waste permit application, liquid effluent retention facility and 200 area effluent treatment facility

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Coenenberg, J.G.

    1997-08-15

    The Hanford Facility Dangerous Waste Permit Application is considered to 10 be a single application organized into a General Information Portion (document 11 number DOE/RL-91-28) and a Unit-Specific Portion. The scope of the 12 Unit-Specific Portion is limited to Part B permit application documentation 13 submitted for individual, `operating` treatment, storage, and/or disposal 14 units, such as the Liquid Effluent Retention Facility and 200 Area Effluent 15 Treatment Facility (this document, DOE/RL-97-03). 16 17 Both the General Information and Unit-Specific portions of the Hanford 18 Facility Dangerous Waste Permit Application address the content of the Part B 19 permit applicationmore » guidance prepared by the Washington State Department of 20 Ecology (Ecology 1987 and 1996) and the U.S. Environmental Protection Agency 21 (40 Code of Federal Regulations 270), with additional information needs 22 defined by the Hazardous and Solid Waste Amendments and revisions of 23 Washington Administrative Code 173-303. For ease of reference, the Washington 24 State Department of Ecology alpha-numeric section identifiers from the permit 25 application guidance documentation (Ecology 1996) follow, in brackets, the 26 chapter headings and subheadings. A checklist indicating where information is 27 contained in the Liquid Effluent Retention Facility and 200 Area Effluent 28 Treatment Facility permit application documentation, in relation to the 29 Washington State Department of Ecology guidance, is located in the Contents 30 Section. 31 32 Documentation contained in the General Information Portion is broader in 33 nature and could be used by multiple treatment, storage, and/or disposal units 34 (e.g., the glossary provided in the General Information Portion). Wherever 35 appropriate, the Liquid Effluent Retention Facility and 200 Area Effluent 36 Treatment Facility permit application documentation makes cross-reference to 37 the General Information Portion, rather than duplicating text. 38 39 Information provided in this Liquid Effluent Retention Facility and 40 200 Area Effluent Treatment Facility permit application documentation is 41 current as of June 1, 1997.« less

  19. 40 CFR 463.33 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS PLASTICS MOLDING AND FORMING POINT SOURCE CATEGORY Finishing Water Subcategory § 463.33 Effluent limitations guidelines representing the degree of effluent reduction...

  20. 40 CFR 463.23 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... representing the degree of effluent reduction attainable by the application of the best available technology... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS PLASTICS MOLDING AND FORMING POINT SOURCE CATEGORY Cleaning Water Subcategory § 463.23 Effluent limitations guidelines representing the degree of effluent reduction...

  1. Application of epigenetic markers in molecular breeding of the swine.

    PubMed

    Zhang, Ke; Feng, Guang-de; Zhang, Bao-yun; Xiang, Wei; Chen, Long; Yang, Fang; Chu, Ming-xing; Wang, Ping-qing

    2016-07-20

    Livestock phenotypes are determined by the interaction of a variety of factors, including the genome, the epigenome and the environment. Epigenetics refers to gene expression changes without DNA sequence alterations. Epigenetic markers mainly include DNA methylation, histone modifications, non-coding RNAs, and imprinting genes. More and more researches show that epigenetic markers play an important role in the traits of pigs by modulating phenotype changes via gene expression. However, the role of epigenetic markers has caught little attention in swine breeding. The mechanism that influences important traits of swine has not been analyzed in detail, and it still lacks adequate scientific basis for practical applications. From the aspects of nutrition, diseases, important economic traits and trans-generational inheritance, we summarize the research, application prospects and challenges in the field of utilizing epigenetic markers in molecular breeding of pigs, thus providing a more comprehensive theoretical basis to promote more rapid research development in this field.

  2. Application and evaluation of enzyme-linked immunosorbent assay and immunoblotting for detection of antibodies to Treponema hyodysenteriae in swine.

    PubMed Central

    Smith, S. C.; Barrett, L. M.; Muir, T.; Christopher, W. L.; Coloe, P. J.

    1991-01-01

    An enzyme-linked immunoassay (ELISA) has been developed to detect serum Immunoglobulin antibodies G and M to Treponema hyodysenteriae in vaccinated, experimentally infected and naturally infected swine. Naturally infected swine gave ELISA titres that were similar to experimentally infected swine, but were significantly less than the titres of vaccinated swine. When serum from naturally infected swine was used to probe nitrocellulose blots of sodium dodecyl sulphate-polyacrylamide gel electrophoresed whole cell proteins of T. hyodysenteriae, the immunoblotting patterns showed IgG antibodies were produced against many T. hyodysenteriae protein antigens and against lipopolysaccharide (LPS). The IgG antibodies directed against LPS were serotype-specific for that LPS and could be used to identify the serotype involved in the T. hyodysenteriae infection in that herd. IgM immunoblots also reacted with the many protein antigens but were less specific for LPS antigen, with a substantial degree of cross-reaction between the LPS of all serotypes. The data demonstrate that a microplate enzyme-linked immunosorbent assay, coupled with immunoblotting, is a very specific and sensitive test for detection of antibody to Treponema hyodysenteriae in swine. Images Fig. 3 Fig. 4 PMID:1936151

  3. Simultaneous removal of colour, phosphorus and disinfection from treated wastewater using an agent synthesized from amorphous silica and hydrated lime.

    PubMed

    Yamashita, Takahiro; Aketo, Tsuyoshi; Minowa, Nobutaka; Sugimoto, Kiyomi; Yokoyama, Hiroshi; Ogino, Akifumi; Tanaka, Yasuo

    2013-01-01

    An agent synthesized from amorphous silica and hydrated lime (CSH-lime) was investigated for its ability to simultaneously remove the colour, phosphorus and disinfection from the effluents from wastewater treatment plants on swine farms. CSH-lime removed the colour and phosphate from the effluents, with the colour-removal effects especially high at pH 12, and phosphorous removal was more effective in strongly alkaline conditions (pH > 10). Colour decreased from 432 +/-111 (mean +/- SD) to 107 +/- 41 colour units and PO4(3-)P was reduced from 45 +/- 39 mg/L to undetectable levels at the CSH-lime dose of 2.0% w/v. Moreover, CSH-lime reduced the total organic carbon from 99.0 to 37.9 mg/L at the dose of 2.0% w/v and was effective at inactivating total heterotrophic and coliform bacteria. However, CSH-lime did not remove nitrogen compounds such as nitrite, nitrate and ammonium. Colour was also removed from dye solutions by CSH-lime, at the same dose.

  4. Control of partial nitrification using pulse aeration for treating digested effluent of swine wastewater.

    PubMed

    Wang, Shuang; Deng, Liangwei; Zheng, Dan; Wang, Lan; Zhang, Yunhong; Yang, Hongnan; Jiang, Yiqi; Huang, Fangyu

    2018-04-22

    Three sequencing batch reactors (SBRs) were used to investigate the influence of pulse frequencies on the partial nitrification (PN) process in this study. At a total aeration time of 6 min each hour, the aerated frequencies of R1, R2 and R3 were 6, 3 and 2 time h -1 . During the steady period (117-143d), the nitrite accumulation rates (NARs) were 90.80%, 90.71% and 90.23% in R1, R2 and R3, respectively, indicating a steady nitritation was acquired. Activity measurements of the sludge samples taken at day 138 showed the activity of nitrite oxidating bacteria (NOB) was 0, indicating NOBs were successfully suppressed. The ratio of NO 2 - -N to NH 4 + -N in the effluent of R3 was 1.35, which most closely matched the influent of Anammox process. However, the energy efficiency evaluation showed that R1 had the highest actual oxygen transfer efficiency (AOTE) and dynamical efficiency (DE). Copyright © 2018 Elsevier Ltd. All rights reserved.

  5. Seasonal variation of macrolide resistance gene abundances in the South Fork Iowa River Watershed

    USDA-ARS?s Scientific Manuscript database

    The Midwestern United States is dominated by agricultural production with high concentrations of swine, leading to application of swine manure onto lands with artificial subsurface drainage. Previous reports have indicated elevated levels of antibiotic resistance genes (ARGs) in surface water and gr...

  6. Molecular Diversity of Bacteroidales in Fecal and Environmental Samples and Swine-Associated Subpopulations

    EPA Science Inventory

    Several swine-specific microbial source tracking methods are based on PCR assays targeting Bacteroidales 16S rRNA gene sequences. The limited application of these assays can be explained by the poor understanding of their molecular diversity in fecal sources and environmental wat...

  7. INNOVATIVE TECHNOLOGY FOR RECYCLING OF MANURE PHOSPHORUS WITH RAPID AMORPHOUS PHOSPHATE PRECIPITATION

    EPA Science Inventory

    Phosphorus (P) recovery from liquid swine manure is an attractive technology when soils in the farm are saturated with P and on-farm land application is not an option. A technology was developed for recovery of soluble P from liquid swine manure as amorphous calcium phosphate (AC...

  8. Myocardial Drug Distribution Generated from Local Epicardial Application: Potential Impact of Cardiac Capillary Perfusion in a Swine Model Using Epinephrine

    PubMed Central

    Maslov, Mikhail Y.; Edelman, Elazer R.; Pezone, Matthew J.; Wei, Abraham E.; Wakim, Matthew G.; Murray, Michael R.; Tsukada, Hisashi; Gerogiannis, Iraklis S.; Groothuis, Adam; Lovich, Mark A.

    2014-01-01

    Prior studies in small mammals have shown that local epicardial application of inotropic compounds drives myocardial contractility without systemic side effects. Myocardial capillary blood flow, however, may be more significant in larger species than in small animals. We hypothesized that bulk perfusion in capillary beds of the large mammalian heart enhances drug distribution after local release, but also clears more drug from the tissue target than in small animals. Epicardial (EC) drug releasing systems were used to apply epinephrine to the anterior surface of the left heart of swine in either point-sourced or distributed configurations. Following local application or intravenous (IV) infusion at the same dose rates, hemodynamic responses, epinephrine levels in the coronary sinus and systemic circulation, and drug deposition across the ventricular wall, around the circumference and down the axis, were measured. EC delivery via point-source release generated transmural epinephrine gradients directly beneath the site of application extending into the middle third of the myocardial thickness. Gradients in drug deposition were also observed down the length of the heart and around the circumference toward the lateral wall, but not the interventricular septum. These gradients extended further than might be predicted from simple diffusion. The circumferential distribution following local epinephrine delivery from a distributed source to the entire anterior wall drove drug toward the inferior wall, further than with point-source release, but again, not to the septum. This augmented drug distribution away from the release source, down the axis of the left ventricle, and selectively towards the left heart follows the direction of capillary perfusion away from the anterior descending and circumflex arteries, suggesting a role for the coronary circulation in determining local drug deposition and clearance. The dominant role of the coronary vasculature is further suggested by the elevated drug levels in the coronary sinus effluent. Indeed, plasma levels, hemodynamic responses, and myocardial deposition remote from the point of release were similar following local EC or IV delivery. Therefore, the coronary vasculature shapes the pharmacokinetics of local myocardial delivery of small catecholamine drugs in large animal models. Optimal design of epicardial drug delivery systems must consider the underlying bulk capillary perfusion currents within the tissue to deliver drug to tissue targets and may favor therapeutic molecules with better potential retention in myocardial tissue. PMID:25234821

  9. 40 CFR 429.52 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... technology (BCT). [Reserved] 429.52 Section 429.52 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS TIMBER PRODUCTS PROCESSING POINT SOURCE CATEGORY Dry Process... by the application of the best conventional pollutant control technology (BCT). [Reserved] ...

  10. 40 CFR 440.52 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... technology currently available (BPT). 440.52 Section 440.52 Protection of Environment ENVIRONMENTAL... reduction attainable by the application of the best practicable control technology currently available (BPT... effluent reduction attainable after application of the best practicable control technology currently...

  11. 40 CFR 440.52 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... technology currently available (BPT). 440.52 Section 440.52 Protection of Environment ENVIRONMENTAL... reduction attainable by the application of the best practicable control technology currently available (BPT... effluent reduction attainable after application of the best practicable control technology currently...

  12. Surface-water quality in agricultural watersheds of the North Carolina Coastal Plain associated with concentrated animal feeding operations

    USGS Publications Warehouse

    Harden, Stephen L.

    2015-01-01

    A classification tree model was developed to examine relations of watershed environmental attributes among the study sites with and without CAFO manure effects. Model results indicated that variations in swine barn density, percentage of wetlands, and total acres available for applying swine-waste manures had an important influence on those watersheds where CAFO effects on water quality were either evident or mitigated. Measurable effects of CAFO waste manures on stream water quality were most evident in those SW and SP watersheds having lower percentages of wetlands combined with higher swine barn densities and (or) higher total acres available for applying waste manure at the swine CAFOs. Stream water quality was similar to background agricultural conditions in SW and SP watersheds with lower swine barn densities coupled with higher percentages of wetlands or lower acres available for swine manure applications. The model provides a useful tool for exploring and identifying similar, unmonitored watersheds in the North Carolina Coastal Plain with potential CAFO manure influences on water quality that might warrant further examination.

  13. 40 CFR 471.66 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) NONFERROUS METALS FORMING AND METAL POWDERS POINT SOURCE CATEGORY Titanium Forming Subcategory § 471.66 Effluent limitations representing the degree of...

  14. 40 CFR 471.66 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) NONFERROUS METALS FORMING AND METAL POWDERS POINT SOURCE CATEGORY Titanium Forming Subcategory § 471.66 Effluent limitations representing the degree of...

  15. 40 CFR 471.66 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) NONFERROUS METALS FORMING AND METAL POWDERS POINT SOURCE CATEGORY Titanium Forming Subcategory § 471.66 Effluent limitations representing the degree of...

  16. 40 CFR 440.92 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Antimony Ore Subcategory § 440.92 Effluent limitations representing the degree of...

  17. 40 CFR 440.112 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Platinum Ores Subcategory § 440.112 Effluent limitations representing the degree of...

  18. 40 CFR 440.112 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Platinum Ores Subcategory § 440.112 Effluent limitations representing the degree of...

  19. 40 CFR 440.92 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Antimony Ore Subcategory § 440.92 Effluent limitations representing the degree of...

  20. 40 CFR 440.112 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Platinum Ores Subcategory § 440.112 Effluent limitations representing the degree of...

  1. 40 CFR 440.92 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Antimony Ore Subcategory § 440.92 Effluent limitations representing the degree of...

  2. Evaluation of five antibiotic resistance genes in wastewater treatment systems of swine farms by real-time PCR.

    PubMed

    Tao, Chi-Wei; Hsu, Bing-Mu; Ji, Wen-Tsai; Hsu, Tsui-Kang; Kao, Po-Min; Hsu, Chun-Po; Shen, Shu-Min; Shen, Tzung-Yu; Wan, Terng-Jou; Huang, Yu-Li

    2014-10-15

    Antibiotics are widely used in livestock for infection treatment and growth promotion. Wastes from animal husbandry are a potential environmental source of antibiotic-insensitive pathogens, and the removal efficiency of the resistance genotypes in current wastewater treatment plants (WWTPs) is unknown. In this study, quantitative PCR was used for evaluating antibiotic resistance genes in wastewater treatment processes. Six wastewater treatment plants in different swine farms were included in this study, and five antibiotic resistance genes (ARGs) were tested for each treatment procedure. All of the tested ARGs including tetA, tetW, sulI, sulII, and blaTEM genes were detected in six swine farms with considerable amounts. The results showed that antibiotic resistance is prevalent in livestock farming. The ARG levels were varied by wastewater treatment procedure, frequently with the highest level at anaerobic treatment tank and lowest in the activated sludge unit and the effluents. After normalizing the ARG levels to 16S rRNA gene copies, the results showed that ARGs in WWTP units fluctuated partly with the quantity of bacteria. Regardless of its importance in biodegradation, the anaerobic procedure may facilitate bacterial growth thus increasing the sustainability of the antibiotic resistance genotypes. After comparing the copy numbers in influx and efflux samples, the mean removal efficiency of ARGs ranged between 33.30 and 97.56%. The results suggested that treatments in the WWTP could partially reduce the spread of antibiotic-resistant bacteria, and additional procedures such as sedimentation may not critically affect the removal efficiency. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Bioenergy from Coastal bermudagrass receiving subsurface drip irrigation with advance-treated swine wastewater.

    PubMed

    Cantrell, Keri B; Stone, Kenneth C; Hunt, Patrick G; Ro, Kyoung S; Vanotti, Matias B; Burns, Joseph C

    2009-07-01

    Coastal bermudagrass (Cynodon dactylon L.) may be a potentially important source of bio-based energy in the southern US due to its vast acreage. It is often produced as part of a waste management plan with varying nutrient composition and energy characteristics on fields irrigated with livestock wastewater. The objective of this study was to determine the effect of subsurface drip irrigation with treated swine wastewater on both the quantity and quality of bermudagrass bioenergy. The treated wastewater was recycled from an advanced treatment system and used for irrigation of bermudagrass in two crop seasons. The experiment had nine water and drip line spacing treatments arrayed in a randomized complete block-design with four replicates. The bermudagrass was analyzed for calorific and mineral contents. Bermudagrass energy yields for 2004 and 2005 ranged from 127.4 to 251.4MJ ha(-1). Compared to irrigation with commercial nitrogen fertilizer, the least biomass energy density was associated with bermudagrass receiving treated swine wastewater. Yet, in 2004 the wastewater irrigated bermudagrass had greater hay yields leading to greater energy yield per ha. This decrease in energy density of wastewater irrigated bermudagrass was associated with increased concentrations of K, Ca, and Na. After thermal conversion, these compounds are known to remain in the ash portion thereby decreasing the energy density. Nonetheless, the loss of energy density using treated effluent via SDI may be offset by the positive influence of these three elements for their catalytic properties in downstream thermal conversion processes such as promoting a lesser char yield and greater combustible gas formation.

  4. Cost-effective treatment of swine wastes through recovery of energy and nutrients.

    PubMed

    Amini, Adib; Aponte-Morales, Veronica; Wang, Meng; Dilbeck, Merrill; Lahav, Ori; Zhang, Qiong; Cunningham, Jeffrey A; Ergas, Sarina J

    2017-11-01

    Wastes from concentrated animal feeding operations (CAFOs) are challenging to treat because they are high in organic matter and nutrients. Conventional swine waste treatment options in the U.S., such as uncovered anaerobic lagoons, result in poor effluent quality and greenhouse gas emissions, and implementation of advanced treatment introduces high costs. Therefore, the purpose of this paper is to evaluate the performance and life cycle costs of an alternative system for treating swine CAFO waste, which recovers valuable energy (as biogas) and nutrients (N, P, K + ) as saleable fertilizers. The system uses in-vessel anaerobic digestion (AD) for methane production and solids stabilization, followed by struvite precipitation and ion exchange (IX) onto natural zeolites (chabazite or clinoptilolite) for nutrient recovery. An alternative approach that integrated struvite recovery and IX into a single reactor, termed STRIEX, was also investigated. Pilot- and bench-scale reactor experiments were used to evaluate the performance of each stage in the treatment train. Data from these studies were integrated into a life cycle cost analysis (LCCA) to assess the cost-effectiveness of various process alternatives. Significant improvement in water quality, high methane production, and high nutrient recovery (generally over 90%) were observed with both the AD-struvite-IX process and the AD-STRIEX process. The LCCA showed that the STRIEX system can provide considerable financial savings compared to conventional systems. AD, however, incurs high capital costs compared to conventional anaerobic lagoons and may require larger scales to become financially attractive. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Horizontal Dissemination of Antimicrobial Resistance Determinants in Multiple Salmonella Serotypes following Isolation from the Commercial Swine Operation Environment after Manure Application.

    PubMed

    Pornsukarom, Suchawan; Thakur, Siddhartha

    2017-10-15

    The aim of this study was to characterize the plasmids carrying antimicrobial resistance (AMR) determinants in multiple Salmonella serotypes recovered from the commercial swine farm environment after manure application on land. Manure and soil samples were collected on day 0 before and after manure application on six farms in North Carolina, and sequential soil samples were recollected on days 7, 14, and 21 from the same plots. All environmental samples were processed for Salmonella , and their plasmid contents were further characterized. A total of 14 isolates including Salmonella enterica serotypes Johannesburg ( n = 2), Ohio ( n = 2), Rissen ( n = 1), Typhimurium var5- ( n = 5), Worthington ( n = 3), and 4,12:i:- ( n = 1), representing different farms, were selected for plasmid analysis. Antimicrobial susceptibility testing was done by broth microdilution against a panel of 14 antimicrobials on the 14 confirmed transconjugants after conjugation assays. The plasmids were isolated by modified alkaline lysis, and PCRs were performed on purified plasmid DNA to identify the AMR determinants and the plasmid replicon types. The plasmids were sequenced for further analysis and to compare profiles and create phylogenetic trees. A class 1 integron with an ANT(2″)-Ia- aadA2 cassette was detected in the 50-kb IncN plasmids identified in S Worthington isolates. We identified 100-kb and 90-kb IncI1 plasmids in S Johannesburg and S Rissen isolates carrying the bla CMY-2 and tet (A) genes, respectively. An identical 95-kb IncF plasmid was widely disseminated among the different serotypes and across different farms. Our study provides evidence on the importance of horizontal dissemination of resistance determinants through plasmids of multiple Salmonella serotypes distributed across commercial swine farms after manure application. IMPORTANCE The horizontal gene transfer of antimicrobial resistance (AMR) determinants located on plasmids is considered to be the main reason for the rapid proliferation and spread of drug resistance. The deposition of manure generated in swine production systems into the environment is identified as a potential source of AMR dissemination. In this study, AMR gene-carrying plasmids were detected in multiple Salmonella serotypes across different commercial swine farms in North Carolina. The plasmid profiles were characterized based on Salmonella serotype donors and incompatibility (Inc) groups. We found that different Inc plasmids showed evidence of AMR gene transfer in multiple Salmonella serotypes. We detected an identical 95-kb plasmid that was widely distributed across swine farms in North Carolina. These conjugable resistance plasmids were able to persist on land after swine manure application. Our study provides strong evidence of AMR determinant dissemination present in plasmids of multiple Salmonella serotypes in the environment after manure application. Copyright © 2017 American Society for Microbiology.

  6. Source tracking swine fecal waste in surface water proximal to swine concentrated animal feeding operations

    PubMed Central

    Heaney, Christopher D.; Myers, Kevin; Wing, Steve; Hall, Devon; Baron, Dothula; Stewart, Jill R.

    2015-01-01

    Swine farming has gone through many changes in the last few decades, resulting in operations with a high animal density known as confined animal feeding operations (CAFOs). These operations produce a large quantity of fecal waste whose environmental impacts are not well understood. The purpose of this study was to investigate microbial water quality in surface waters proximal to swine CAFOs including microbial source tracking of fecal microbes specific to swine. For one year, surface water samples at up- and downstream sites proximal to swine CAFO lagoon waste land application sites were tested for fecal indicator bacteria (fecal coliforms, Escherichia coli and Enterococcus) and candidate swine-specific microbial source-tracking (MST) markers (Bacteroidales Pig-1-Bac, Pig-2-Bac, and Pig-Bac-2, and methanogen P23-2). Testing of 187 samples showed high fecal indicator bacteria concentrations at both up- and downstream sites. Overall, 40%, 23%, and 61% of samples exceeded state and federal recreational water quality guidelines for fecal coliforms, E. coli, and Enterococcus, respectively. Pig-1-Bac and Pig-2-Bac showed the highest specificity to swine fecal wastes and were 2.47 (95% confidence interval [CI] = 1.03, 5.94) and 2.30 times (95% CI = 0.90, 5.88) as prevalent proximal down- than proximal upstream of swine CAFOs, respectively. Pig-1-Bac and Pig-2-Bac were also 2.87 (95% CI = 1.21, 6.80) and 3.36 (95% CI = 1.34, 8.41) times as prevalent when 48 hour antecedent rainfall was greater than versus less than the mean, respectively. Results suggest diffuse and overall poor sanitary quality of surface waters where swine CAFO density is high. Pig-1-Bac and Pig-2-Bac are useful for tracking off-site conveyance of swine fecal wastes into surface waters proximal to and downstream of swine CAFOs and during rain events. PMID:25600418

  7. Source tracking swine fecal waste in surface water proximal to swine concentrated animal feeding operations.

    PubMed

    Heaney, Christopher D; Myers, Kevin; Wing, Steve; Hall, Devon; Baron, Dothula; Stewart, Jill R

    2015-04-01

    Swine farming has gone through many changes in the last few decades, resulting in operations with a high animal density known as confined animal feeding operations (CAFOs). These operations produce a large quantity of fecal waste whose environmental impacts are not well understood. The purpose of this study was to investigate microbial water quality in surface waters proximal to swine CAFOs including microbial source tracking of fecal microbes specific to swine. For one year, surface water samples at up- and downstream sites proximal to swine CAFO lagoon waste land application sites were tested for fecal indicator bacteria (fecal coliforms, Escherichia coli and Enterococcus) and candidate swine-specific microbial source-tracking (MST) markers (Bacteroidales Pig-1-Bac, Pig-2-Bac, and Pig-Bac-2, and methanogen P23-2). Testing of 187 samples showed high fecal indicator bacteria concentrations at both up- and downstream sites. Overall, 40%, 23%, and 61% of samples exceeded state and federal recreational water quality guidelines for fecal coliforms, E. coli, and Enterococcus, respectively. Pig-1-Bac and Pig-2-Bac showed the highest specificity to swine fecal wastes and were 2.47 (95% confidence interval [CI]=1.03, 5.94) and 2.30 times (95% CI=0.90, 5.88) as prevalent proximal down- than proximal upstream of swine CAFOs, respectively. Pig-1-Bac and Pig-2-Bac were also 2.87 (95% CI=1.21, 6.80) and 3.36 (95% CI=1.34, 8.41) times as prevalent when 48 hour antecedent rainfall was greater than versus less than the mean, respectively. Results suggest diffuse and overall poor sanitary quality of surface waters where swine CAFO density is high. Pig-1-Bac and Pig-2-Bac are useful for tracking off-site conveyance of swine fecal wastes into surface waters proximal to and downstream of swine CAFOs and during rain events. Copyright © 2014 Elsevier B.V. All rights reserved.

  8. 40 CFR 469.19 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... technology (BCT). 469.19 Section 469.19 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE CATEGORY Semiconductor... application of the best conventional pollution control technology (BCT). Except as provided in 40 CFR 125.30...

  9. 40 CFR 469.29 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... technology (BCT). 469.29 Section 469.29 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ELECTRICAL AND ELECTRONIC COMPONENTS POINT SOURCE CATEGORY Electronic... by the application of the best conventional pollution control technology (BCT). Except as provided in...

  10. 40 CFR 425.53 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... achievable (BAT). 425.53 Section 425.53 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... by the application of the best available technology economically achievable (BAT). Except as provided... of the best available technology economically achievable (BAT): The effluent limitations are those...

  11. 40 CFR 430.54 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... achievable (BAT). 430.54 Section 430.54 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... attainable by the application of best available technology economically achievable (BAT). Except as provided... of the best available technology economically achievable (BAT). (a)(1) The following effluent...

  12. 40 CFR 425.53 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... achievable (BAT). 425.53 Section 425.53 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... by the application of the best available technology economically achievable (BAT). Except as provided... of the best available technology economically achievable (BAT): The effluent limitations are those...

  13. 40 CFR 425.73 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... achievable (BAT). 425.73 Section 425.73 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... application of the best available technology economically achievable (BAT). Except as provided in 40 CFR 125... best available technology economically achievable (BAT): The effluent limitations are those for Total...

  14. 40 CFR 469.15 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... achievable (BAT). 469.15 Section 469.15 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... by the application of the best available technology economically achievable (BAT). Except as provided... of the best available technology economically achievable (BAT): Subpart A—Semiconductor BAT Effluent...

  15. 40 CFR 425.83 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... achievable (BAT). 425.83 Section 425.83 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... application of the best available technology economically achievable (BAT). Except as provided in 40 CFR 125... best available technology economically achievable (BAT): The effluent limitations are those for Total...

  16. 40 CFR 425.83 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... achievable (BAT). 425.83 Section 425.83 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... application of the best available technology economically achievable (BAT). Except as provided in 40 CFR 125... best available technology economically achievable (BAT): The effluent limitations are those for Total...

  17. 40 CFR 425.73 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... achievable (BAT). 425.73 Section 425.73 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... application of the best available technology economically achievable (BAT). Except as provided in 40 CFR 125... best available technology economically achievable (BAT): The effluent limitations are those for Total...

  18. 40 CFR 430.54 - Effluent limitations representing the degree of effluent reduction attainable by the application...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... achievable (BAT). 430.54 Section 430.54 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... attainable by the application of best available technology economically achievable (BAT). Except as provided... of the best available technology economically achievable (BAT). (a)(1) The following effluent...

  19. 40 CFR 406.53 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... technology economically achievable: there shall be no discharge of process waste water pollutants to... representing the degree of effluent reduction attainable by the application of the best available technology... by the application of the best available technology economically achievable. The following...

  20. 40 CFR 426.33 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... technology economically achievable: There shall be no discharge of process waste water pollutants to... representing the degree of effluent reduction attainable by the application of the best available technology... attainable by the application of the best available technology economically achievable. The following...

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