Sample records for tagged site sts

  1. STS-26 crewmembers eat on middeck as TAGS printout drifts among them

    NASA Technical Reports Server (NTRS)

    1988-01-01

    STS-26 Commander Frederick H. Hauck (center) reviews lengthy text and graphics system (TAGS) printout as it drifts across the middeck while his fellow crewmembers (left to right) Mission Specialist (MS) David C. Hilmers, MS George D. Nelson, and Pilot Richard O. Covey eat various snack items. The open airlock hatch and the sleep restraints on the starboard wall are visible in the background.

  2. High-resolution melt analysis to identify and map sequence-tagged site anchor points onto linkage maps: a white lupin (Lupinus albus) map as an exemplar.

    PubMed

    Croxford, Adam E; Rogers, Tom; Caligari, Peter D S; Wilkinson, Michael J

    2008-01-01

    * The provision of sequence-tagged site (STS) anchor points allows meaningful comparisons between mapping studies but can be a time-consuming process for nonmodel species or orphan crops. * Here, the first use of high-resolution melt analysis (HRM) to generate STS markers for use in linkage mapping is described. This strategy is rapid and low-cost, and circumvents the need for labelled primers or amplicon fractionation. * Using white lupin (Lupinus albus, x = 25) as a case study, HRM analysis was applied to identify 91 polymorphic markers from expressed sequence tag (EST)-derived and genomic libraries. Of these, 77 generated STS anchor points in the first fully resolved linkage map of the species. The map also included 230 amplified fragment length polymorphisms (AFLP) loci, spanned 1916 cM (84.2% coverage) and divided into the expected 25 linkage groups. * Quantitative trait loci (QTL) analyses performed on the population revealed genomic regions associated with several traits, including the agronomically important time to flowering (tf), alkaloid synthesis and stem height (Ph). Use of HRM-STS markers also allowed us to make direct comparisons between our map and that of the related crop, Lupinus angustifolius, based on the conversion of RFLP, microsatellite and single nucleotide polymorphism (SNP) markers into HRM markers.

  3. Genomic integrity of the Y chromosome sequence-tagged-sites in infertile and Down syndrome Jordanian males.

    PubMed

    Yasin, S R; Tahtamouni, L H; Najeeb, N S; Issa, N M; Al-Mazaydeh, Z A; Alfaouri, A A

    2014-09-01

    The long arm of the Y chromosome contains nonoverlapping regions termed azoospermia factor (AZF) with great influence on male fertility. Microdeletions at these regions minimise the males' ability to father offsprings. In this preliminary study, we attempted to screen the presence or absence of twenty Y chromosome's sequence-tagged sites (STS) associated with fertility in infertile and Down syndrome (DS) males. Genomic DNA from 35 fertile, 74 infertile and 22 karyotyped DS males was extracted and amplified in multiplex polymerase chain reaction (PCR) containing 20 primer pairs that amplify Y-specific STS that cover functional regions associated with AZF and spermatogenesis-related genes. Our results indicated the integrity of the Y chromosome at the 20 fertility markers for both the fertile and Down syndrome males. However, the results of the infertile males showed the presence of microdeletions at these Y-specific STS. Three samples showed Y chromosome microdeletion when blood and seminal fluid genomic DNA were assayed, while two samples showed microdeletion only when seminal fluid genomic DNA was assayed. The current study demonstrated that the molecular genetic aspect of infertility should be given proper attention when dealing with infertility cases. Furthermore, our results indicate the importance of genetic counselling in managing infertility cases. © 2013 Blackwell Verlag GmbH.

  4. Application of the High Resolution Melting analysis for genetic mapping of Sequence Tagged Site markers in narrow-leafed lupin (Lupinus angustifolius L.).

    PubMed

    Kamel, Katarzyna A; Kroc, Magdalena; Święcicki, Wojciech

    2015-01-01

    Sequence tagged site (STS) markers are valuable tools for genetic and physical mapping that can be successfully used in comparative analyses among related species. Current challenges for molecular markers genotyping in plants include the lack of fast, sensitive and inexpensive methods suitable for sequence variant detection. In contrast, high resolution melting (HRM) is a simple and high-throughput assay, which has been widely applied in sequence polymorphism identification as well as in the studies of genetic variability and genotyping. The present study is the first attempt to use the HRM analysis to genotype STS markers in narrow-leafed lupin (Lupinus angustifolius L.). The sensitivity and utility of this method was confirmed by the sequence polymorphism detection based on melting curve profiles in the parental genotypes and progeny of the narrow-leafed lupin mapping population. Application of different approaches, including amplicon size and a simulated heterozygote analysis, has allowed for successful genetic mapping of 16 new STS markers in the narrow-leafed lupin genome.

  5. 40 CFR 35.4040 - How many groups can receive a TAG at one Superfund site?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false How many groups can receive a TAG at one Superfund site? 35.4040 Section 35.4040 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Eligible? § 35.4040 How many groups can receive a TAG at one Superfund site? (a) Only one TAG may be...

  6. 40 CFR 35.4040 - How many groups can receive a TAG at one Superfund site?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 1 2013-07-01 2013-07-01 false How many groups can receive a TAG at one Superfund site? 35.4040 Section 35.4040 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Eligible? § 35.4040 How many groups can receive a TAG at one Superfund site? (a) Only one TAG may be...

  7. 40 CFR 35.4040 - How many groups can receive a TAG at one Superfund site?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 1 2014-07-01 2014-07-01 false How many groups can receive a TAG at one Superfund site? 35.4040 Section 35.4040 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Eligible? § 35.4040 How many groups can receive a TAG at one Superfund site? (a) Only one TAG may be...

  8. 40 CFR 35.4040 - How many groups can receive a TAG at one Superfund site?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 1 2012-07-01 2012-07-01 false How many groups can receive a TAG at one Superfund site? 35.4040 Section 35.4040 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Eligible? § 35.4040 How many groups can receive a TAG at one Superfund site? (a) Only one TAG may be...

  9. 40 CFR 35.4040 - How many groups can receive a TAG at one Superfund site?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 1 2011-07-01 2011-07-01 false How many groups can receive a TAG at one Superfund site? 35.4040 Section 35.4040 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Eligible? § 35.4040 How many groups can receive a TAG at one Superfund site? (a) Only one TAG may be...

  10. Quantum tagging for tags containing secret classical data

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kent, Adrian

    Various authors have considered schemes for quantum tagging, that is, authenticating the classical location of a classical tagging device by sending and receiving quantum signals from suitably located distant sites, in an environment controlled by an adversary whose quantum information processing and transmitting power is potentially unbounded. All of the schemes proposed elsewhere in the literature assume that the adversary is able to inspect the interior of the tagging device. All of these schemes have been shown to be breakable if the adversary has unbounded predistributed entanglement. We consider here the case in which the tagging device contains a finitemore » key string shared with distant sites but kept secret from the adversary, and show this allows the location of the tagging device to be authenticated securely and indefinitely. Our protocol relies on quantum key distribution between the tagging device and at least one distant site, and demonstrates a new practical application of quantum key distribution. It also illustrates that the attainable security in position-based cryptography can depend crucially on apparently subtle details in the security scenario considered.« less

  11. Tagging studies of mule deer fawns on the Hanford Site, 1969 to 1977

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Eberhardt, L.E.; Hedlund, J.D.; Rickard, W.H.

    1979-10-01

    From 1969 to 1977, 346 mule deer (Odocoileus hemionus) fawns were tagged and released on islands and shoreline habitat associated with the Columbia River on the Hanford Site in south-central Washington. The purpose was to determine the movement of mule deer along the Columbia River shoreline from the Hanford Site through tag recovery. Twenty-one tagged deer have been killed primarily by hunters near the Hanford Site or on areas of the Hanford Site open to public access. Movements of up to 113 km from Hanford have been documented. Although the Columbia River at Hanford is one of the largest andmore » most swift-flowing rivers in North America it is not an impassable barrier to mule deer. River islands are important and perhaps critical fawining habitat for the local deer herd. The selection of these islands by pregnant female deer is apparently influenced by predation, human access, and recreational use of islands. The number of fawns captured decreased during the latter years of the study (1974 to 1977). This is probably a reflection of an actual decrease in deer productivity, particularly along the upper stretch of the Columbia flowing through the Hanford Site. The reasons for this apparent decrease are unkown.« less

  12. Movement patterns of juvenile whale sharks tagged at an aggregation site in the Red Sea.

    PubMed

    Berumen, Michael L; Braun, Camrin D; Cochran, Jesse E M; Skomal, Gregory B; Thorrold, Simon R

    2014-01-01

    Conservation efforts aimed at the whale shark, Rhincodon typus, remain limited by a lack of basic information on most aspects of its ecology, including global population structure, population sizes and movement patterns. Here we report on the movements of 47 Red Sea whale sharks fitted with three types of satellite transmitting tags from 2009-2011. Most of these sharks were tagged at a single aggregation site near Al-Lith, on the central coast of the Saudi Arabian Red Sea. Individuals encountered at this site were all juveniles based on size estimates ranging from 2.5-7 m total length with a sex ratio of approximately 1∶1. All other known aggregation sites for juvenile whale sharks are dominated by males. Results from tagging efforts showed that most individuals remained in the southern Red Sea and that some sharks returned to the same location in subsequent years. Diving data were recorded by 37 tags, revealing frequent deep dives to at least 500 m and as deep as 1360 m. The unique temperature-depth profiles of the Red Sea confirmed that several whale sharks moved out of the Red Sea while tagged. The wide-ranging horizontal movements of these individuals highlight the need for multinational, cooperative efforts to conserve R. typus populations in the Red Sea and Indian Ocean.

  13. Movement Patterns of Juvenile Whale Sharks Tagged at an Aggregation Site in the Red Sea

    PubMed Central

    Berumen, Michael L.; Braun, Camrin D.; Cochran, Jesse E. M.; Skomal, Gregory B.; Thorrold, Simon R.

    2014-01-01

    Conservation efforts aimed at the whale shark, Rhincodon typus, remain limited by a lack of basic information on most aspects of its ecology, including global population structure, population sizes and movement patterns. Here we report on the movements of 47 Red Sea whale sharks fitted with three types of satellite transmitting tags from 2009–2011. Most of these sharks were tagged at a single aggregation site near Al-Lith, on the central coast of the Saudi Arabian Red Sea. Individuals encountered at this site were all juveniles based on size estimates ranging from 2.5–7 m total length with a sex ratio of approximately 1∶1. All other known aggregation sites for juvenile whale sharks are dominated by males. Results from tagging efforts showed that most individuals remained in the southern Red Sea and that some sharks returned to the same location in subsequent years. Diving data were recorded by 37 tags, revealing frequent deep dives to at least 500 m and as deep as 1360 m. The unique temperature-depth profiles of the Red Sea confirmed that several whale sharks moved out of the Red Sea while tagged. The wide-ranging horizontal movements of these individuals highlight the need for multinational, cooperative efforts to conserve R. typus populations in the Red Sea and Indian Ocean. PMID:25076407

  14. STS-69 Crew members display 'Dog Crew' patches

    NASA Technical Reports Server (NTRS)

    1995-01-01

    Following their arrival at KSC's Shuttle Landing Facility, the five astronauts assigned to Space Shuttle Mission STS-69 display the unofficial crew patch for their upcoming spaceflight: the Dog Crew II patch. Mission Commander David M. Walker (center) and Payload Commander James S. Voss (second from right) previously flew together on Mission STS-53, the final dedicated Department of Defense flight on the Space Shuttle. A close comradery formed among Walker, Voss and the rest of the crew, and they dubbed themselves the 'dogs of war', with each of the STS-53 'Dog Crew' members assigned a 'dog tag' or nickname. When the STS-69 astronauts also became good buddies, they decided it was time for the Dog Crew II to be named. Walker's dog tag is Red Dog, Voss's is Dogface, Pilot Kenneth D. Cockrell (second from left) is Cujo, space rookie and Mission Specialist Michael L. Gernhardt (left) is Under Dog, and Mission Specialist James H. Newman (right) is Pluato. The Dog Crew II patch features a bulldog peering out from a doghouse shaped like the Space Shuttle and lists the five crew member's dog names. The five astronauts are scheduled to lift off on the fifth Shuttle flight of the year at 11:04 a.m. EDT, August 31, aboard the Space Shuttle Endeavour.

  15. Pilot Fullerton reviews FDF and TAGS printout on forward flight deck

    NASA Image and Video Library

    1982-03-30

    STS003-23-180 (22-30 March 1982) --- Astronaut Gordon Fullerton, STS-3 pilot, wearing communications kit assembly (ASSY) mini-headset (HDST), reviews flight data file (FDF) checklist and text and graphics system (TAGS) printout (ticker tape) while in pilots ejection seat (S2). Pilot station control panels F4, F7, F8, O3, window shade, and portable oxygen system (POS) assy appear in view. Photo credit: NASA

  16. The STS-93 external tank and booster stack sits at the Mobile Launcher Platform park site

    NASA Technical Reports Server (NTRS)

    1999-01-01

    The STS-93 stack of solid rocket boosters and external tank sits at the Mobile Launcher Platform park site waiting for lightning shield wires to be installed on the Vehicle Assembly Building (VAB) in the background. The stack is being temporarily stored outside the VAB while Space Shuttle Discovery undergoes repair to hail damage in High Bay 1. Discovery was rolled back from Pad 39B to the VAB for repairs because access to all of the damaged areas was not possible at the pad. The STS-93 stack will be moved under the wires at the VAB for protection until Discovery returns to the pad, later this week. The scheduled date for launch of mission STS-96 is no earlier than May 27. STS-93 is targeted for launch on July 22, carrying the Chandra X-ray Observatory.

  17. Identification of a rice gene (Bph 1) conferring resistance to brown planthopper (Nilaparvata lugens Stal) using STS markers.

    PubMed

    Kim, Suk-Man; Sohn, Jae-Keun

    2005-08-31

    This study was carried out to identify a high-resolution marker for a gene conferring resistance to brown planthopper (BPH) biotype 1, using japonica type resistant lines. Bulked segregant analyses were conducted using 520 RAPD primers to identify RAPD fragments linked to the BPH resistance gene. Eleven RAPDs were shown to be polymorphic amplicons between resistant and susceptible progeny. One of these primers, OPE 18, which amplified a 923 bp band tightly linked to resistance, was converted into a sequence-tagged-site (STS) marker. The STS marker, BpE18-3, was easily detectable as a dominant band with tight linkage (3.9cM) to Bph1. It promises to be useful as a marker for assisted selection of resistant progeny in backcross breeding programs to introgress the resistance gene into elite japonica cultivars.

  18. An active site-tail interaction in the structure of hexahistidine-tagged Thermoplasma acidophilum citrate synthase

    DOE PAGES

    Murphy, Jesse R.; Donini, Stefano; Kappock, T. Joseph

    2015-10-01

    Citrate synthase (CS) plays a central metabolic role in aerobes and many other organisms. The CS reaction comprises two half-reactions: a Claisen aldol condensation of acetyl-CoA (AcCoA) and oxaloacetate (OAA) that forms citryl-CoA (CitCoA), and CitCoA hydrolysis. Protein conformational changes that `close' the active site play an important role in the assembly of a catalytically competent condensation active site. CS from the thermoacidophile Thermoplasma acidophilum (TpCS) possesses an endogenous Trp fluorophore that can be used to monitor the condensation reaction. The 2.2 Å resolution crystal structure of TpCS fused to a C-terminal hexahistidine tag (TpCSH6) reported here is an `open'more » structure that, when compared with several liganded TpCS structures, helps to define a complete path for active-site closure. One active site in each dimer binds a neighboring His tag, the first nonsubstrate ligand known to occupy both the AcCoA and OAA binding sites. Solution data collectively suggest that this fortuitous interaction is stabilized by the crystalline lattice. In conclusion, as a polar but almost neutral ligand, the active site-tail interaction provides a new starting point for the design of bisubstrate-analog inhibitors of CS.« less

  19. An active site-tail interaction in the structure of hexahistidine-tagged Thermoplasma acidophilum citrate synthase.

    PubMed

    Murphy, Jesse R; Donini, Stefano; Kappock, T Joseph

    2015-10-01

    Citrate synthase (CS) plays a central metabolic role in aerobes and many other organisms. The CS reaction comprises two half-reactions: a Claisen aldol condensation of acetyl-CoA (AcCoA) and oxaloacetate (OAA) that forms citryl-CoA (CitCoA), and CitCoA hydrolysis. Protein conformational changes that `close' the active site play an important role in the assembly of a catalytically competent condensation active site. CS from the thermoacidophile Thermoplasma acidophilum (TpCS) possesses an endogenous Trp fluorophore that can be used to monitor the condensation reaction. The 2.2 Å resolution crystal structure of TpCS fused to a C-terminal hexahistidine tag (TpCSH6) reported here is an `open' structure that, when compared with several liganded TpCS structures, helps to define a complete path for active-site closure. One active site in each dimer binds a neighboring His tag, the first nonsubstrate ligand known to occupy both the AcCoA and OAA binding sites. Solution data collectively suggest that this fortuitous interaction is stabilized by the crystalline lattice. As a polar but almost neutral ligand, the active site-tail interaction provides a new starting point for the design of bisubstrate-analog inhibitors of CS.

  20. STS upper stage operations

    NASA Technical Reports Server (NTRS)

    Kitchens, M. D.; Schnyer, A. D.

    1977-01-01

    Several design/development and operational approaches for STS upper stages are being pursued to realize maximum operational and economic benefits upon the introduction of the STS in the 1980s. The paper focuses special attention on safety operations, launch site operations and on-orbit operations.

  1. Movement of tagged dredged sand at thalweg disposal sites in the Upper Mississippi River. Volume 2. Savanna Bay and Duck Creek sites

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Paddock, R.A.; McCown, D.L.

    Experiments were conducted on the Upper Mississippi River at two sites, Savanna Bay and Duck Creek, to investigate the movement of dredged sand after disposal in the thalweg. These experiments are part of a larger study, which includes similar experiments conducted earlier at two other sites on the Upper Mississippi River. At the Savanna Bay site, hydraulically dredged sand was tagged with sand coated with fluorescent dye before being deposited as a pile in the thalweg. Bathymetric surveys and surficial bottom sampling were conducted on five occasions over a nine-month period following disposal to identify changes in the topography ofmore » the pile and to detect the presence of dyed sand. At the Duck Creek site, only bathymetric surveys were conducted. The general behavior of the disposal piles at these two new sites was similar to that of the piles at the first two sites. Topographic evidence of the piles disappeared, and bottom conditions similar to the predisposal conditions were reestablished following the first period of high river flows after disposal. At Savanna Bay, as had happened at the first two sites, the tagged sand remained in the main river channel as it moved downstream in response to flood currents and did not migrate into nearby border areas, backwaters, or sloughs. 6 references, 89 figures, 2 tables.« less

  2. The effect of drag and attachment site of external tags on swimming eels: experimental quantification and evaluation tool.

    PubMed

    Tudorache, Christian; Burgerhout, Erik; Brittijn, Sebastiaan; van den Thillart, Guido

    2014-01-01

    Telemetry studies on aquatic animals often use external tags to monitor migration patterns and help to inform conservation effort. However, external tags are known to impair swimming energetics dramatically in a variety of species, including the endangered European eel. Due to their high swimming efficiency, anguilliform swimmers are very susceptibility for added drag. Using an integration of swimming physiology, behaviour and kinematics, we investigated the effect of additional drag and site of externally attached tags on swimming mode and costs. The results show a significant effect of a) attachment site and b) drag on multiple energetic parameters, such as Cost Of Transport (COT), critical swimming speed (Ucrit) and optimal swimming speed (Uopt), possibly due to changes in swimming kinematics. Attachment at 0.125 bl from the tip of the snout is a better choice than at the Centre Of Mass (0.35 bl), as it is the case in current telemetry studies. Quantification of added drag effect on COT and Ucrit show a (limited) correlation, suggesting that the Ucrit test can be used for evaluating external tags for telemetry studies until a certain threshold value. Uopt is not affected by added drag, validating previous findings of telemetry studies. The integrative methodology and the evaluation tool presented here can be used for the design of new studies using external telemetry tags, and the (re-) evaluation of relevant studies on anguilliform swimmers.

  3. Media blitz of mission STS-95 fills grounds around Press Site

    NASA Technical Reports Server (NTRS)

    1998-01-01

    The day before the launch of mission STS-95, the Press Site was inundated with 40 trailers, 75 trucks and RVs, 8 stages and 8 risers to accommodate the 3,750 media requests to cover the launch and return to space of John H. Glenn Jr., a senator from Ohio. Glenn flew aboard Friendship 7 in February 1962, and was the first American to orbit the Earth. Glenn is one of a crew of seven on board Space Shuttle Discovery for the nine-day mission.

  4. The Effect of Drag and Attachment Site of External Tags on Swimming Eels: Experimental Quantification and Evaluation Tool

    PubMed Central

    Tudorache, Christian; Burgerhout, Erik; Brittijn, Sebastiaan; van den Thillart, Guido

    2014-01-01

    Telemetry studies on aquatic animals often use external tags to monitor migration patterns and help to inform conservation effort. However, external tags are known to impair swimming energetics dramatically in a variety of species, including the endangered European eel. Due to their high swimming efficiency, anguilliform swimmers are very susceptibility for added drag. Using an integration of swimming physiology, behaviour and kinematics, we investigated the effect of additional drag and site of externally attached tags on swimming mode and costs. The results show a significant effect of a) attachment site and b) drag on multiple energetic parameters, such as Cost Of Transport (COT), critical swimming speed (Ucrit) and optimal swimming speed (Uopt), possibly due to changes in swimming kinematics. Attachment at 0.125 bl from the tip of the snout is a better choice than at the Centre Of Mass (0.35 bl), as it is the case in current telemetry studies. Quantification of added drag effect on COT and Ucrit show a (limited) correlation, suggesting that the Ucrit test can be used for evaluating external tags for telemetry studies until a certain threshold value. Uopt is not affected by added drag, validating previous findings of telemetry studies. The integrative methodology and the evaluation tool presented here can be used for the design of new studies using external telemetry tags, and the (re-) evaluation of relevant studies on anguilliform swimmers. PMID:25409179

  5. PIT tags increase effectiveness of freshwater mussel recaptures

    USGS Publications Warehouse

    Kurth, J.; Loftin, C.; Zydlewski, Joseph D.; Rhymer, Judith

    2007-01-01

    Translocations are used increasingly to conserve populations of rare freshwater mussels. Recovery of translocated mussels is essential to accurate assessment of translocation success. We designed an experiment to evaluate the use of passive integrated transponder (PIT) tags to mark and track individual freshwater mussels. We used eastern lampmussels (Lampsilis radiata radiata) as a surrogate for 2 rare mussel species. We assessed internal and external PIT-tag retention in the laboratory and field. Internal tag retention was high (75-100%), and tag rejection occurred primarily during the first 3 wk after tagging. A thin layer of nacre coated internal tags 3 to 4 mo after insertion, suggesting that long-term retention is likely. We released mussels with external PIT tags at 3 field study sites and recaptured them with a PIT pack (mobile interrogation unit) 8 to 10 mo and 21 to 23 mo after release. Numbers of recaptured mussels differed among study sites; however, we found more tagged mussels with the PIT-pack searches with visual confirmation (72-80%) than with visual searches alone (30-47%) at all sites. PIT tags offer improved recapture of translocated mussels and increased accuracy of posttranslocation monitoring. ?? 2007 by The North American Benthological Society.

  6. sts003-010-613

    NASA Image and Video Library

    2009-06-24

    STS003-010-613 (22-30 March 1982) --- A truly remarkable view of White Sands and the nearby Carrizozo Lava Beds in southeast NM (33.5N, 106.5W). White Sands, site of the WW II atomic bomb development and testing facility and later post war nuclear weapons testing that can still be seen in the cleared circular patterns on the ground. Space shuttle Columbia (STS-3), this mission, landed at the White Sands alternate landing site because of bad weather at Edwards AFB, CA. Photo credit: NASA

  7. A Methodological Framework to Estimate the Site Fidelity of Tagged Animals Using Passive Acoustic Telemetry

    PubMed Central

    Capello, Manuela; Robert, Marianne; Soria, Marc; Potin, Gael; Itano, David; Holland, Kim; Deneubourg, Jean-Louis; Dagorn, Laurent

    2015-01-01

    The rapid expansion of the use of passive acoustic telemetry technologies has facilitated unprecedented opportunities for studying the behavior of marine organisms in their natural environment. This technological advance would greatly benefit from the parallel development of dedicated methodologies accounting for the variety of timescales involved in the remote detection of tagged animals related to instrumental, environmental and behavioral events. In this paper we propose a methodological framework for estimating the site fidelity (“residence times”) of acoustic tagged animals at different timescales, based on the survival analysis of continuous residence times recorded at multiple receivers. Our approach is validated through modeling and applied on two distinct datasets obtained from a small coastal pelagic species (bigeye scad, Selar crumenophthalmus) and a large, offshore pelagic species (yellowfin tuna, Thunnus albacares), which show very distinct spatial scales of behavior. The methodological framework proposed herein allows estimating the most appropriate temporal scale for processing passive acoustic telemetry data depending on the scientific question of interest. Our method provides residence times free of the bias inherent to environmental and instrumental noise that can be used to study the small scale behavior of acoustic tagged animals. At larger timescales, it can effectively identify residence times that encompass the diel behavioral excursions of fish out of the acoustic detection range. This study provides a systematic framework for the analysis of passive acoustic telemetry data that can be employed for the comparative study of different species and study sites. The same methodology can be used each time discrete records of animal detections of any nature are employed for estimating the site fidelity of an animal at different timescales. PMID:26261985

  8. A Methodological Framework to Estimate the Site Fidelity of Tagged Animals Using Passive Acoustic Telemetry.

    PubMed

    Capello, Manuela; Robert, Marianne; Soria, Marc; Potin, Gael; Itano, David; Holland, Kim; Deneubourg, Jean-Louis; Dagorn, Laurent

    2015-01-01

    The rapid expansion of the use of passive acoustic telemetry technologies has facilitated unprecedented opportunities for studying the behavior of marine organisms in their natural environment. This technological advance would greatly benefit from the parallel development of dedicated methodologies accounting for the variety of timescales involved in the remote detection of tagged animals related to instrumental, environmental and behavioral events. In this paper we propose a methodological framework for estimating the site fidelity ("residence times") of acoustic tagged animals at different timescales, based on the survival analysis of continuous residence times recorded at multiple receivers. Our approach is validated through modeling and applied on two distinct datasets obtained from a small coastal pelagic species (bigeye scad, Selar crumenophthalmus) and a large, offshore pelagic species (yellowfin tuna, Thunnus albacares), which show very distinct spatial scales of behavior. The methodological framework proposed herein allows estimating the most appropriate temporal scale for processing passive acoustic telemetry data depending on the scientific question of interest. Our method provides residence times free of the bias inherent to environmental and instrumental noise that can be used to study the small scale behavior of acoustic tagged animals. At larger timescales, it can effectively identify residence times that encompass the diel behavioral excursions of fish out of the acoustic detection range. This study provides a systematic framework for the analysis of passive acoustic telemetry data that can be employed for the comparative study of different species and study sites. The same methodology can be used each time discrete records of animal detections of any nature are employed for estimating the site fidelity of an animal at different timescales.

  9. Verification of STS markers for leaf rust resistance genes of wheat by seven European laboratories.

    PubMed

    Błaszczyk, Lidia; Chełkowski, Jerzy; Korzun, Victor; Kraic, Jan; Ordon, Frank; Ovesná, Jaroslava; Purnhauser, Laszlo; Tar, Melinda; Vida, Gyula

    2004-01-01

    A set of Thatcher near-isogenic lines and two breeding lines were used to examine sequence tagged site (STS) markers linked to leaf rust resistance genes Lr9, Lr10, Lr19, Lr24, Lr28, Lr29, Lr35, and a simple sequenced repeat (SSR) marker for Lr39. The selected STS markers for resistance genes Lr9, Lr10, Lr19, Lr24 and Lr28 were identified in seven accessions by seven European laboratories. Near-isogenic lines of the spring wheat Thatcher were used as positive controls. Markers for resistance genes Lr9, Lr10, Lr19, Lr24 were identified in all seven laboratories as amplification products of 1100 bp, 310 bp, 130 bp and 310 bp, respectively. The STS markers linked to resistance genes Lr9, Lr10, Lr19, Lr24, Lr29, Lr35 and the SSR marker for Lr39 were robust and highly specific for these genes and will be useful in marker-assisted selection in wheat. However, the amplification product of 378 bp that corresponded with resistance gene Lr28 was detected in all accessions including genotypes lacking this gene in all seven laboratories. This marker needs to be improved.

  10. Transferability of STS markers in studying genetic relationships of marvel grass (Dichanthium annulatum).

    PubMed

    Saxena, Raghvendra; Chandra, Amaresh

    2011-11-01

    Transferability of sequence-tagged-sites (STS) markers was assessed for genetic relationships study among accessions of marvel grass (Dichanthium annulatum Forsk.). In total, 17 STS primers of Stylosanthes origin were tested for their reactivity with thirty accessions of Dichanthium annulatum. Of these, 14 (82.4%) reacted and a total 106 (84 polymorphic) bands were scored. The number of bands generated by individual primer pairs ranged from 4 to 11 with an average of 7.57 bands, whereas polymorphic bands ranged from 4 to 9 with an average of 6.0 bands accounts to an average polymorphism of 80.1%. Polymorphic information content (PIC) ranged from 0.222 to 0.499 and marker index (MI) from 1.33 to 4.49. Utilizing Dice coefficient of genetic similarity dendrogram was generated through un-weighted pairgroup method with arithmetic mean (UPGMA) algorithm. Further, clustering through sequential agglomerative hierarchical and nested (SAHN) method resulted three main clusters constituted all accessions except IGBANG-D-2. Though there was intermixing of few accessions of one agro-climatic region to another, largely groupings of accessions were with their regions of collections. Bootstrap analysis at 1000 scale also showed large number of nodes (11 to 17) having strong clustering (> 50). Thus, results demonstrate the utility of STS markers of Stylosanthes in studying the genetic relationships among accessions of Dichanthium.

  11. Tag-to-Tag Interference Suppression Technique Based on Time Division for RFID.

    PubMed

    Khadka, Grishma; Hwang, Suk-Seung

    2017-01-01

    Radio-frequency identification (RFID) is a tracking technology that enables immediate automatic object identification and rapid data sharing for a wide variety of modern applications using radio waves for data transmission from a tag to a reader. RFID is already well established in technical areas, and many companies have developed corresponding standards and measurement techniques. In the construction industry, effective monitoring of materials and equipment is an important task, and RFID helps to improve monitoring and controlling capabilities, in addition to enabling automation for construction projects. However, on construction sites, there are many tagged objects and multiple RFID tags that may interfere with each other's communications. This reduces the reliability and efficiency of the RFID system. In this paper, we propose an anti-collision algorithm for communication between multiple tags and a reader. In order to suppress interference signals from multiple neighboring tags, the proposed algorithm employs the time-division (TD) technique, where tags in the interrogation zone are assigned a specific time slot so that at every instance in time, a reader communicates with tags using the specific time slot. We present representative computer simulation examples to illustrate the performance of the proposed anti-collision technique for multiple RFID tags.

  12. Substantial prevalence of microdeletions of the Y-chromosome in infertile men with idiopathic azoospermia and oligozoospermia detected using a sequence-tagged site-based mapping strategy

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Najmabadi, H.; Huang, V.; Bhasin, D.

    1996-04-01

    Genes on the long arm of Y (Yq), particularly within interval 6, are believed to play a critical role in human spermatogenesis. Cytogenetically detectable deletions of this region are associated with azoospermia in men, but are relatively uncommon. The objective of this study was to validate a sequence-tagged site (STS)-mapping strategy for the detection of Yq microdeletions and to use this method to determine the proportion of men with idiopathic azoospermia or severe oligozoospermia who carry microdeletions in Yq. STS mapping of a sufficiently large sample of infertile men should also help further localize the putative gene(s) involved in themore » pathogenesis of male infertility. Genomic DNA was extracted from peripheral leukocytes of 16 normal fertile men, 7 normal fertile women, 60 infertile men, and 15 patients with the X-linked disorder, ichthyosis. PCR primers were synthesized for 26 STSs that span Yq interval 6. None of the 16 normal men of known fertility had microdeletions. Seven normal fertile women failed to amplify any of the 26 STSs, providing evidence of their Y specificity. No microdeletions were detected in any of the 15 patients with ichthyosis. Of the 60 infertile men typed with 26 STSs, 11 (18%; 10 azoospermic and 1 oligozoospermic) failed to amplify 1 or more STS. Interestingly, 4 of the 11 patients had microdeletions in a region that is outside the Yq region from which the DAZ (deleted in azoospermia gene region) gene was cloned. In an additional 3 patients, microdeletions were present both inside and outside the DAZ region. The physical locations of these microdeletions provide further support for the concept that a gene(s) on Yq deletion interval 6 plays an important role in spermatogenesis. The presence of deletions that do not overlap with the DAZ region suggests that genes other than the DAZ gene may also be implicated in the pathogenesis of some subsets of male infertility. 48 refs., 2 figs., 2 tabs.« less

  13. The use of tags in monitoring limits on mobile missiles

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Fetter, S.

    1987-03-01

    Three tagging systems were considered in this paper: as a supplement to on-site inspection (OSI), as a supplement to national technical means (NTM), and as a supplement to site surveillance systems. Each system would require a different type of tag, perhaps ranging from microchip tags with infrared transponders to navigation receivers. Use of tags as a supplement to OSIs may be the simplest system to implement because it places the least demands on technology. Tags may make OSI more acceptable by replacing humans with remote sensors, thereby decreasing the perceived potential for espionage. Using tags as a supplement to NTMmore » decreases the necessity for human OSI even further, but places higher demands on technology and may affect the normal operation of deployment areas. Site surveillance systems using tags have the potential for excellent missile verification, but they may be excessively intrusive and expensive, and could have a large effect on the normal operation of declared facilities.« less

  14. All quiet at Press Site before media blitz of mission STS-95

    NASA Technical Reports Server (NTRS)

    1998-01-01

    On a normal day of activity prior to the launch of mission STS- 95, the parking lot (foreground), plus the grandstand and buildings at the Press Site (beyond and to the right), are easily located from the air. The view gives no indication of the media frenzy the launch would generate with the return to space of John H. Glenn Jr., a senator from Ohio, whose first flight was aboard Friendship 7 in February 1962, and the first American to orbit the Earth. Glenn is one of a crew of seven on board Space Shuttle Discovery for the nine-day mission.

  15. Poly(A)-tag deep sequencing data processing to extract poly(A) sites.

    PubMed

    Wu, Xiaohui; Ji, Guoli; Li, Qingshun Quinn

    2015-01-01

    Polyadenylation [poly(A)] is an essential posttranscriptional processing step in the maturation of eukaryotic mRNA. The advent of next-generation sequencing (NGS) technology has offered feasible means to generate large-scale data and new opportunities for intensive study of polyadenylation, particularly deep sequencing of the transcriptome targeting the junction of 3'-UTR and the poly(A) tail of the transcript. To take advantage of this unprecedented amount of data, we present an automated workflow to identify polyadenylation sites by integrating NGS data cleaning, processing, mapping, normalizing, and clustering. In this pipeline, a series of Perl scripts are seamlessly integrated to iteratively map the single- or paired-end sequences to the reference genome. After mapping, the poly(A) tags (PATs) at the same genome coordinate are grouped into one cleavage site, and the internal priming artifacts removed. Then the ambiguous region is introduced to parse the genome annotation for cleavage site clustering. Finally, cleavage sites within a close range of 24 nucleotides and from different samples can be clustered into poly(A) clusters. This procedure could be used to identify thousands of reliable poly(A) clusters from millions of NGS sequences in different tissues or treatments.

  16. A suite of standard post-tagging evaluation metrics can help assess tag retention for field-based fish telemetry research

    USGS Publications Warehouse

    Gerber, Kayla M.; Mather, Martha E.; Smith, Joseph M.

    2017-01-01

    Telemetry can inform many scientific and research questions if a context exists for integrating individual studies into the larger body of literature. Creating cumulative distributions of post-tagging evaluation metrics would allow individual researchers to relate their telemetry data to other studies. Widespread reporting of standard metrics is a precursor to the calculation of benchmarks for these distributions (e.g., mean, SD, 95% CI). Here we illustrate five types of standard post-tagging evaluation metrics using acoustically tagged Blue Catfish (Ictalurus furcatus) released into a Kansas reservoir. These metrics included: (1) percent of tagged fish detected overall, (2) percent of tagged fish detected daily using abacus plot data, (3) average number of (and percent of available) receiver sites visited, (4) date of last movement between receiver sites (and percent of tagged fish moving during that time period), and (5) number (and percent) of fish that egressed through exit gates. These metrics were calculated for one to three time periods: early (<10 d), during (weekly), and at the end of the study (5 months). Over three-quarters of our tagged fish were detected early (85%) and at the end (85%) of the study. Using abacus plot data, all tagged fish (100%) were detected at least one day and 96% were detected for > 5 days early in the study. On average, tagged Blue Catfish visited 9 (50%) and 13 (72%) of 18 within-reservoir receivers early and at the end of the study, respectively. At the end of the study, 73% of all tagged fish were detected moving between receivers. Creating statistical benchmarks for individual metrics can provide useful reference points. In addition, combining multiple metrics can inform ecology and research design. Consequently, individual researchers and the field of telemetry research can benefit from widespread, detailed, and standard reporting of post-tagging detection metrics.

  17. The use of tags and tag clouds to discern credible content in online health message forums.

    PubMed

    O'Grady, Laura; Wathen, C Nadine; Charnaw-Burger, Jill; Betel, Lisa; Shachak, Aviv; Luke, Robert; Hockema, Stephen; Jadad, Alejandro R

    2012-01-01

    Web sites with health-oriented content are potentially harmful if inaccurate or inappropriate medical information is used to make health-related decisions. Checklists, rating systems and guidelines have been developed to help people determine what is credible, but recent Internet technologies emphasize applications that are collaborative in nature, including tags and tag clouds, where site users 'tag' or label online content, each using their own labelling system. Concepts such as the date, reference, author, testimonial and quotations are considered predictors of credible content. An understanding of these descriptive tools, how they relate to the depiction of credibility and how this relates to overall efforts to label data in relation to the semantic web has yet to emerge. This study investigates how structured (pre-determined) and unstructured (user-generated) tags and tag clouds with a multiple word search feature are used by participants to assess credibility of messages posted in online message forums. The targeted respondents were those using web sites message forums for disease self-management. We also explored the relevancy of our findings to the labelling or indexing of data in the context of the semantic web. Diabetes was chosen as the content area in this study, since (a) this is a condition with increasing prevalence and (b) diabetics have been shown to actively use the Internet to manage their condition. From January to March 2010 participants were recruited using purposive sampling techniques. A screening instrument was used to determine eligibility. The study consisted of a demographic and computer usage survey, a series of usability tests and an interview. We tested participants (N=22) on two scenarios, each involving tasks that assessed their ability to tag content and search using a tag cloud that included six structured credibility terms (statistics, date, reference, author, testimonial and quotations). MORAE Usability software (version 3

  18. Dual-mode fluorophore-doped nickel nitrilotriacetic acid-modified silica nanoparticles combine histidine-tagged protein purification with site-specific fluorophore labeling.

    PubMed

    Kim, Sung Hoon; Jeyakumar, M; Katzenellenbogen, John A

    2007-10-31

    We present the first example of a fluorophore-doped nickel chelate surface-modified silica nanoparticle that functions in a dual mode, combining histidine-tagged protein purification with site-specific fluorophore labeling. Tetramethylrhodamine (TMR)-doped silica nanoparticles, estimated to contain 700-900 TMRs per ca. 23 nm particle, were surface modified with nitrilotriacetic acid (NTA), producing TMR-SiO2-NTA-Ni2+. Silica-embedded TMR retains very high quantum yield, is resistant to quenching by buffer components, and is modestly quenched and only to a certain depth (ca. 2 nm) by surface-attached Ni2+. When exposed to a bacterial lysate containing estrogen receptor alpha ligand binding domain (ERalpha) as a minor component, these beads showed very high specificity binding, enabling protein purification in one step. The capacity and specificity of these beads for binding a his-tagged protein were characterized by electrophoresis, radiometric counting, and MALDI-TOF MS. ERalpha, bound to TMR-SiO2-NTA-Ni++ beads in a site-specific manner, exhibited good activity for ligand binding and for ligand-induced binding to coactivators in solution FRET experiments and protein microarray fluorometric and FRET assays. This dual-mode type TMR-SiO2-NTA-Ni2+ system represents a powerful combination of one-step histidine-tagged protein purification and site-specific labeling with multiple fluorophore species.

  19. OSIRIS-REx Touch-And-Go (TAG) Navigation Performance

    NASA Technical Reports Server (NTRS)

    Berry, Kevin; Antreasian, Peter; Moreau, Michael C.; May, Alex; Sutter, Brian

    2015-01-01

    The Origins Spectral Interpretation Resource identification Security Regolith Explorer (OSIRIS-REx) mission is a NASA New Frontiers mission launching in 2016 to rendezvous with the near-Earth asteroid (101955) Bennu in late 2018. Following an extensive campaign of proximity operations activities to characterize the properties of Bennu and select a suitable sample site, OSIRIES-REx will fly a Touch-And-Go (TAG) trajectory to the asteroid's surface to obtain a regolith sample. The paper summarizes the mission design of the TAG sequence, the propulsive required to achieve the trajectory, and the sequence of events leading up to the TAG event. The paper will summarize the Monte-Carlo simulation of the TAG sequence and present analysis results that demonstrate the ability to conduct the TAG within 25 meters of the selected sample site and +-2 cms of the targeted contact velocity. The paper will describe some of the challenges associated with conducting precision navigation operations and ultimately contacting a very small asteroid.

  20. OSIRI-REx Touch and Go (TAG) Navigation Performance

    NASA Technical Reports Server (NTRS)

    Berry, Kevin; Antreasian, Peter; Moreau, Michael C.; May, Alex; Sutter, Brian

    2015-01-01

    The Origins Spectral Interpretation Resource Identification Security Regolith Explorer (OSIRIS-REx) mission is a NASA New Frontiers mission launching in 2016 to rendezvous with the near-Earth asteroid (101955) Bennu in late 2018. Following an extensive campaign of proximity operations activities to characterize the properties of Bennu and select a suitable sample site, OSIRIS-REx will fly a Touch-And-Go (TAG) trajectory to the asteroid's surface to obtain a regolith sample. The paper summarizes the mission design of the TAG sequence, the propulsive maneuvers required to achieve the trajectory, and the sequence of events leading up to the TAG event. The paper also summarizes the Monte-Carlo simulation of the TAG sequence and presents analysis results that demonstrate the ability to conduct the TAG within 25 meters of the selected sample site and 2 cm/s of the targeted contact velocity. The paper describes some of the challenges associated with conducting precision navigation operations and ultimately contacting a very small asteroid.

  1. Measurement of tag confidence in user generated contents retrieval

    NASA Astrophysics Data System (ADS)

    Lee, Sihyoung; Min, Hyun-Seok; Lee, Young Bok; Ro, Yong Man

    2009-01-01

    As online image sharing services are becoming popular, the importance of correctly annotated tags is being emphasized for precise search and retrieval. Tags created by user along with user-generated contents (UGC) are often ambiguous due to the fact that some tags are highly subjective and visually unrelated to the image. They cause unwanted results to users when image search engines rely on tags. In this paper, we propose a method of measuring tag confidence so that one can differentiate confidence tags from noisy tags. The proposed tag confidence is measured from visual semantics of the image. To verify the usefulness of the proposed method, experiments were performed with UGC database from social network sites. Experimental results showed that the image retrieval performance with confidence tags was increased.

  2. Cetacean Tag Design Workshop Held in Arlington, Virginia on 16-17 March 2009

    DTIC Science & Technology

    2009-03-01

    tagging to assess tag performance and animal health . • Examine performance of current tag attachment designs by assessing holding power and anchoring...tag attachment performance, and potentially adversely affect animal health . The second recommended step was to test and collect baseline data on...the tag attachment site to assess animal health and tag performance. Best to test tags on populations that are not listed as threatened or

  3. 40 CFR 35.4165 - When does EPA award a TAG?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 1 2012-07-01 2012-07-01 false When does EPA award a TAG? 35.4165... does EPA award a TAG? (a) EPA may award TAGs throughout the Superfund process, including during... the site is proposed for listing on the NPL. (b) Based on the availability of funds, EPA may delay...

  4. Views of STS-9 crewmembers with families upon return to Ellington

    NASA Technical Reports Server (NTRS)

    1983-01-01

    Astronaut John W. Young, STS-9 commander, is joined by his wife Susy at Ellington Air Force Base following arrival from the STS-9 landing site in southern California (45650); Astronaut Brewster H. Shaw Jr., STS-9 pilot, hugs his wife Kathleen and children Brewster III, right, Jessica and Brandon at ELlington AFB (45651).

  5. The Evaluation of the Impact of Age, Skin Tags, Metabolic Syndrome, Body Mass Index, and Smoking on Homocysteine, Endothelin-1, High-sensitive C-reactive Protein, and on the Heart.

    PubMed

    El Safoury, Omar Soliman; Ezzat, Marwa; Abdelhamid, Mahmoud F; Shoukry, Nadia; Badawy, Ehssan

    2013-07-01

    Skin tags (STs) are small, pedunculated skin-colored or brown papules that occur around any site where skin folds occur. The literature is short of comprehensive and controlled clinical studies aimed to evaluate the atherogenic risk factors in patients with STs. The aim of this study is to evaluate the impact of age, STs, metabolic syndrome (METs), body mass index (BMI), and smoking on homocysteine (Hcy), endothelin-1 (ET-1), high-sensitive C-reactive protein (Hs-CRP), and on cardiovascular diseases. This study included 30 cardiac patients with STs, 30 non-cardiac patients with STs, and 30 healthy controls with neither heart disease nor STs. History of smoking, measurement of height, weight, BMI, waist circumference (WC), blood pressure, STs number, color, acanthosis nigricans, estimation of serum level of fasting glucose, triglycerides (TGs), cholesterol, high-dense lipoproteins (HDL), Hcy, ET-1, Hs-CRP, and the presence of the METs were elicited in the three groups. Regarding the Hcy, ET-1, and Hs-CRP, the cardiac-STs group showed the highest levels and the control group showed the least (P < 0.001). The percents of patients with METs were 56.7% in the cardiac-STs, 40% in the non-cardiac-STs, and 0% in the control group (P < 0.001). Mean BMI exceeded the limit of obesity in the cardiac-STs group (30.9 ± 3.9) and the non-cardiac-STs group (32.6 ± 6) and was normal in the control group (24.7 ± 2.8). Hyperpigmented STs were present in 66.7% of the cardiac-STs group. Multivariate regression analysis for the independent effectors on Hcy level were the presence of STs (P < 0.001), METs (P = 0.001), and BMI (P = 0.024). Regarding ET-1, the effectors were the presence of STs and METs (P = 0.032). For Hs-CRP, effectors were the presence of STs (P < 0.001) and smoking (P = 0.040). Multivariate logistic regression of the predictors of cardiac disease showed that the independent predictors of the occurrence of cardiac disease were BMI (P < 0.001), STs (P = 0.002), and

  6. A Study of HTML Title Tag Creation Behavior of Academic Web Sites

    ERIC Educational Resources Information Center

    Noruzi, Alireza

    2007-01-01

    The HTML title tag information should identify and describe exactly what a Web page contains. This paper analyzes the "Title element" and raises a significant question: "Why is the title tag important?" Search engines base search results and page rankings on certain criteria. Among the most important criteria is the presence of the search keywords…

  7. sts132-s-005

    NASA Image and Video Library

    2010-05-14

    STS132-S-005 (14 May 2010) --- Witnessed by news media representatives and STS-132 Tweet-up participants on hand by the countdown clock at the Press Site, Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

  8. sts132-s-011

    NASA Image and Video Library

    2010-05-14

    STS132-S-011 (14 May 2010) --- Witnessed by news media representatives and STS-132 Tweet-up participants on hand by the countdown clock at the Press Site, Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

  9. sts132-s-010

    NASA Image and Video Library

    2010-05-14

    STS132-S-010 (14 May 2010) --- Witnessed by news media representatives and STS-132 Tweet-up participants on hand by the countdown clock at the Press Site, Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

  10. In situ tagging technique for fishes provides insight into growth and movement of invasive lionfish

    PubMed Central

    Akins, John L; Morris, James A; Green, Stephanie J

    2014-01-01

    Information on fish movement and growth is primarily obtained through the marking and tracking of individuals with external tags, which are usually affixed to anesthetized individuals at the surface. However, the quantity and quality of data obtained by this method is often limited by small sample sizes owing to the time associated with the tagging process, high rates of tagging-related mortality, and displacement of tagged individuals from the initial capture location. To address these issues, we describe a technique for applying external streamer and dart tags in situ, which uses SCUBA divers to capture and tag individual fish on the sea floor without the use of anesthetic. We demonstrate this method for Indo-Pacific lionfish (Pterois volitans/P. miles), species which are particularly vulnerable to barotrauma when transported to and handled at the surface. To test our method, we tagged 161 individuals inhabiting 26 coral reef locations in the Bahamas over a period of 3 years. Our method resulted in no instances of barotrauma, reduced handling and recovery time, and minimal post-tagging release displacement compared with conventional ex situ tag application. Opportunistic resighting and recapture of tagged individuals reveals that lionfish exhibit highly variable site fidelity, movement patterns, and growth rates on invaded coral reef habitats. In total, 24% of lionfish were resighted between 29 and 188 days after tagging. Of these, 90% were located at the site of capture, while the remaining individuals were resighted between 200 m and 1.1 km from initial site of capture over 29 days later. In situ growth rates ranged between 0.1 and 0.6 mm/day. While individuals tagged with streamer tags posted slower growth rates with increasing size, as expected, there was no relationship between growth rate and fish size for individuals marked with dart tags, potentially because of large effects of tag presence on the activities of small bodied lionfish (i.e., <150

  11. In situ tagging technique for fishes provides insight into growth and movement of invasive lionfish.

    PubMed

    Akins, John L; Morris, James A; Green, Stephanie J

    2014-10-01

    Information on fish movement and growth is primarily obtained through the marking and tracking of individuals with external tags, which are usually affixed to anesthetized individuals at the surface. However, the quantity and quality of data obtained by this method is often limited by small sample sizes owing to the time associated with the tagging process, high rates of tagging-related mortality, and displacement of tagged individuals from the initial capture location. To address these issues, we describe a technique for applying external streamer and dart tags in situ, which uses SCUBA divers to capture and tag individual fish on the sea floor without the use of anesthetic. We demonstrate this method for Indo-Pacific lionfish (Pterois volitans/P. miles), species which are particularly vulnerable to barotrauma when transported to and handled at the surface. To test our method, we tagged 161 individuals inhabiting 26 coral reef locations in the Bahamas over a period of 3 years. Our method resulted in no instances of barotrauma, reduced handling and recovery time, and minimal post-tagging release displacement compared with conventional ex situ tag application. Opportunistic resighting and recapture of tagged individuals reveals that lionfish exhibit highly variable site fidelity, movement patterns, and growth rates on invaded coral reef habitats. In total, 24% of lionfish were resighted between 29 and 188 days after tagging. Of these, 90% were located at the site of capture, while the remaining individuals were resighted between 200 m and 1.1 km from initial site of capture over 29 days later. In situ growth rates ranged between 0.1 and 0.6 mm/day. While individuals tagged with streamer tags posted slower growth rates with increasing size, as expected, there was no relationship between growth rate and fish size for individuals marked with dart tags, potentially because of large effects of tag presence on the activities of small bodied lionfish (i.e., <150

  12. Synaptic Tagging During Memory Allocation

    PubMed Central

    Rogerson, Thomas; Cai, Denise; Frank, Adam; Sano, Yoshitake; Shobe, Justin; Aranda, Manuel L.; Silva, Alcino J.

    2014-01-01

    There is now compelling evidence that the allocation of memory to specific neurons (neuronal allocation) and synapses (synaptic allocation) in a neurocircuit is not random and that instead specific mechanisms, such as increases in neuronal excitability and synaptic tagging and capture, determine the exact sites where memories are stored. We propose an integrated view of these processes, such that neuronal allocation, synaptic tagging and capture, spine clustering and metaplasticity reflect related aspects of memory allocation mechanisms. Importantly, the properties of these mechanisms suggest a set of rules that profoundly affect how memories are stored and recalled. PMID:24496410

  13. Tags Help Make Libraries Del.icio.us: Social Bookmarking and Tagging Boost Participation

    ERIC Educational Resources Information Center

    Rethlefsen, Melissa L.

    2007-01-01

    Traditional library web products, whether online public access catalogs, library databases, or even library web sites, have long been rigidly controlled and difficult to use. Patrons regularly prefer Google's simple interface. Now social bookmarking and tagging tools help librarians bridge the gap between the library's need to offer authoritative,…

  14. The Use of Social Tags in Text and Image Searching on the Web

    ERIC Educational Resources Information Center

    Kim, Yong-Mi

    2011-01-01

    In recent years, tags have become a standard feature on a diverse range of sites on the Web, accompanying blog posts, photos, videos, and online news stories. Tags are descriptive terms attached to Internet resources. Despite the rapid adoption of tagging, how people use tags during the search process is not well understood. There is little…

  15. The Evaluation of the Impact of Age, Skin Tags, Metabolic Syndrome, Body Mass Index, and Smoking on Homocysteine, Endothelin-1, High-sensitive C-reactive Protein, and on the Heart

    PubMed Central

    El Safoury, Omar Soliman; Ezzat, Marwa; Abdelhamid, Mahmoud F; Shoukry, Nadia; Badawy, Ehssan

    2013-01-01

    Background: Skin tags (STs) are small, pedunculated skin-colored or brown papules that occur around any site where skin folds occur. The literature is short of comprehensive and controlled clinical studies aimed to evaluate the atherogenic risk factors in patients with STs. Aim of Work: The aim of this study is to evaluate the impact of age, STs, metabolic syndrome (METs), body mass index (BMI), and smoking on homocysteine (Hcy), endothelin-1 (ET-1), high-sensitive C-reactive protein (Hs-CRP), and on cardiovascular diseases. Materials and Methods: This study included 30 cardiac patients with STs, 30 non-cardiac patients with STs, and 30 healthy controls with neither heart disease nor STs. History of smoking, measurement of height, weight, BMI, waist circumference (WC), blood pressure, STs number, color, acanthosis nigricans, estimation of serum level of fasting glucose, triglycerides (TGs), cholesterol, high-dense lipoproteins (HDL), Hcy, ET-1, Hs-CRP, and the presence of the METs were elicited in the three groups. Results: Regarding the Hcy, ET-1, and Hs-CRP, the cardiac-STs group showed the highest levels and the control group showed the least (P < 0.001). The percents of patients with METs were 56.7% in the cardiac-STs, 40% in the non-cardiac-STs, and 0% in the control group (P < 0.001). Mean BMI exceeded the limit of obesity in the cardiac-STs group (30.9 ± 3.9) and the non-cardiac-STs group (32.6 ± 6) and was normal in the control group (24.7 ± 2.8). Hyperpigmented STs were present in 66.7% of the cardiac-STs group. Multivariate regression analysis for the independent effectors on Hcy level were the presence of STs (P < 0.001), METs (P = 0.001), and BMI (P = 0.024). Regarding ET-1, the effectors were the presence of STs and METs (P = 0.032). For Hs-CRP, effectors were the presence of STs (P < 0.001) and smoking (P = 0.040). Multivariate logistic regression of the predictors of cardiac disease showed that the independent predictors of the occurrence of

  16. Fabrication of Nanometer- and Micrometer-Scale Protein Structures by Site-Specific Immobilization of Histidine-Tagged Proteins to Aminosiloxane Films with Photoremovable Protein-Resistant Protecting Groups

    PubMed Central

    2016-01-01

    The site-specific immobilization of histidine-tagged proteins to patterns formed by far-field and near-field exposure of films of aminosilanes with protein-resistant photolabile protecting groups is demonstrated. After deprotection of the aminosilane, either through a mask or using a scanning near-field optical microscope, the amine terminal groups are derivatized first with glutaraldehyde and then with N-(5-amino-1-carboxypentyl)iminodiacetic acid to yield a nitrilo-triacetic-acid-terminated surface. After complexation with Ni2+, this surface binds histidine-tagged GFP and CpcA-PEB in a site-specific fashion. The chemistry is simple and reliable and leads to extensive surface functionalization. Bright fluorescence is observed in fluorescence microscopy images of micrometer- and nanometer-scale patterns. X-ray photoelectron spectroscopy is used to study quantitatively the efficiency of photodeprotection and the reactivity of the modified surfaces. The efficiency of the protein binding process is investigated quantitatively by ellipsometry and by fluorescence microscopy. We find that regions of the surface not exposed to UV light bind negligible amounts of His-tagged proteins, indicating that the oligo(ethylene glycol) adduct on the nitrophenyl protecting group confers excellent protein resistance; in contrast, exposed regions bind His-GFP very effectively, yielding strong fluorescence that is almost completely removed on treatment of the surface with imidazole, confirming a degree of site-specific binding in excess of 90%. This simple strategy offers a versatile generic route to the spatially selective site-specific immobilization of proteins at surfaces. PMID:26820378

  17. King Ata Tag Mountain Range, western China as seen by STS-66 Atlantis

    NASA Image and Video Library

    1994-11-14

    The northwest-southeast trending, snow-covered, linear mountain range is the King Ata Tag Mountain, located in extreme western most China. Highest peaks are just over 20,100 feet above sea level. The town or village of Muji is located in the largest river valley that is south of this range of mountains. This area includes part of the country of Tajikistan. The center point of the photo is approximately 39.30 degrees north and 74.30 degrees easth.

  18. L-Area STS MTR/NRU/NRX Grapple Assembly Closure Mechanics Review

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Huizenga, D. J.

    2016-06-08

    A review of the closure mechanics associated with the Shielded Transfer System (STS) MTR/NRU/NRX grapple assembly utilized at the Savannah River Site (SRS) was performed. This review was prompted by an operational event which occurred at the Canadian Nuclear Laboratories (CNL) utilizing a DTS-XL grapple assembly which is essentially identical to the STS MTR/NRU/NRX grapple assembly used at the SRS. The CNL operational event occurred when a NRU/NRX fuel basket containing spent nuclear fuel assemblies was inadvertently released by the DTS-XL grapple assembly during a transfer. The SM review of the STS MTR/NRU/NRX grapple assembly will examine the operational aspectsmore » of the STS and the engineered features of the STS which prevent such an event at the SRS. The design requirements for the STS NRU/NRX modifications and the overall layout of the STS are provided in other documents.« less

  19. Fabrication of nanometer- and micrometer-scale protein structures by site-specific immobilization of histidine-tagged proteins to aminosiloxane films with photoremovable protein-resistant protecting groups

    DOE PAGES

    Xia, Sijing; Cartron, Michael; Morby, James; ...

    2016-01-28

    The site-specific immobilization of histidine-tagged proteins to patterns formed by far-field and near-field exposure of films of aminosilanes with protein-resistant photolabile protecting groups is demonstrated. After deprotection of the aminosilane, either through a mask or using a scanning near-field optical microscope, the amine terminal groups are derivatized first with glutaraldehyde and then with N-(5-amino-1-carboxypentyl)iminodiacetic acid to yield a nitrilo-triacetic-acid-terminated surface. After complexation with Ni 2+, this surface binds histidine-tagged GFP and CpcA-PEB in a site-specific fashion. The chemistry is simple and reliable and leads to extensive surface functionalization. Bright fluorescence is observed in fluorescence microscopy images of micrometer- and nanometer-scalemore » patterns. X-ray photoelectron spectroscopy is used to study quantitatively the efficiency of photodeprotection and the reactivity of the modified surfaces. The efficiency of the protein binding process is investigated quantitatively by ellipsometry and by fluorescence microscopy. We find that regions of the surface not exposed to UV light bind negligible amounts of His-tagged proteins, indicating that the oligo(ethylene glycol) adduct on the nitrophenyl protecting group confers excellent protein resistance; in contrast, exposed regions bind His-GFP very effectively, yielding strong fluorescence that is almost completely removed on treatment of the surface with imidazole, confirming a degree of site-specific binding in excess of 90%. As a result, this simple strategy offers a versatile generic route to the spatially selective site-specific immobilization of proteins at surfaces.« less

  20. Stable isotope, site-specific mass tagging for protein identification

    DOEpatents

    Chen, Xian

    2006-10-24

    Proteolytic peptide mass mapping as measured by mass spectrometry provides an important method for the identification of proteins, which are usually identified by matching the measured and calculated m/z values of the proteolytic peptides. A unique identification is, however, heavily dependent upon the mass accuracy and sequence coverage of the fragment ions generated by peptide ionization. The present invention describes a method for increasing the specificity, accuracy and efficiency of the assignments of particular proteolytic peptides and consequent protein identification, by the incorporation of selected amino acid residue(s) enriched with stable isotope(s) into the protein sequence without the need for ultrahigh instrumental accuracy. Selected amino acid(s) are labeled with .sup.13C/.sup.15N/.sup.2H and incorporated into proteins in a sequence-specific manner during cell culturing. Each of these labeled amino acids carries a defined mass change encoded in its monoisotopic distribution pattern. Through their characteristic patterns, the peptides with mass tag(s) can then be readily distinguished from other peptides in mass spectra. The present method of identifying unique proteins can also be extended to protein complexes and will significantly increase data search specificity, efficiency and accuracy for protein identifications.

  1. NASA Administrator, U.S. Secretary of State watch STS-88 launch

    NASA Technical Reports Server (NTRS)

    1998-01-01

    At the Banana Creek Viewing Site, NASA Administrator Daniel Goldin (left), U.S. Secretary of State Madeleine Albright (center) and astronaut Michael Lopez-Alegria watch the launch of STS-88 from Launch Pad 39A at 3:35:34 a.m. EST. STS-88 is the first U.S. mission dedicated to the assembly of the International Space Station (ISS). Lopez-Alegria is part of the STS-92 crew that is assigned to the fourth ISS assembly flight scheduled for launch on Oct. 28, 1999, aboard Discovery.

  2. Radio tag retention and tag-related mortality among adult sockeye salmon

    USGS Publications Warehouse

    Ramstad, Kristina M.; Woody, Carol Ann

    2003-01-01

    Tag retention and tag-related mortality are concerns for any tagging study but are rarely estimated. We assessed retention and mortality rates for esophageal radio tag implants in adult sockeye salmon Oncorhynchus nerka. Migrating sockeye salmon captured at the outlet of Lake Clark, Alaska, were implanted with one of four different radio tags (14.5 × 43 mm (diameter × length), 14.5 × 49 mm, 16 × 46 mm, and 19 × 51 mm). Fish were observed for 15 to 35 d after tagging to determine retention and mortality rates. The overall tag retention rate was high (0.98; 95% confidence interval (CI), 0.92-1.00; minimum, 33 d), with one loss of a 19-mm × 51- mm tag. Mortality of tagged sockeye salmon (0.02; 95% CI, 0-0.08) was similar to that of untagged controls (0.03 (0-0.15)). Sockeye salmon with body lengths (mid-eye to tail fork) of 585-649 mm retained tags as large as 19 × 51 mm and those with body lengths of 499-628 mm retained tags as small as 14.5 × 43 mm for a minimum of 33 d with no increase in mortality. The tags used in this study represent a suite of radio tags that vary in size, operational life, and cost but that are effective in tracking adult anadromous salmon with little tag loss or increase in fish mortality.

  3. Shuttle flight data and in-flight anomaly list. STS-1 through STS-50, and STS-52 through STS-56. Revision T

    NASA Technical Reports Server (NTRS)

    1993-01-01

    This report contains mission data for space shuttle flights and consists of three sections. The first section is a listing of shuttle flight data for flights STS-1 through STS-55 gathered during the mission evaluation process. The second section is a listing of all orbiter in-flight anomalies arranged in order by affected Work Unit Codes of the failed items from shuttle flights STS-1 through STS-50 and STS-52 through STS-56. The third section consists of data derived from the as-flown orbiter attitude timelines and crew activity plans for each mission. The data are presented in chart form and show the progression of the mission from launch to entry interface with the varying orbiter attitudes (roll, pitch, and yaw) and the time duration in each attitude. The chart also shows the orbiter's velocity vector, i.e., which of the orbiter's body axes is pointing forward along the orbital path. The Beta angle, the angle between the sun vector and the orbital plane, is also shown for each 12-hour period of the mission.

  4. Extracting tag hierarchies.

    PubMed

    Tibély, Gergely; Pollner, Péter; Vicsek, Tamás; Palla, Gergely

    2013-01-01

    Tagging items with descriptive annotations or keywords is a very natural way to compress and highlight information about the properties of the given entity. Over the years several methods have been proposed for extracting a hierarchy between the tags for systems with a "flat", egalitarian organization of the tags, which is very common when the tags correspond to free words given by numerous independent people. Here we present a complete framework for automated tag hierarchy extraction based on tag occurrence statistics. Along with proposing new algorithms, we are also introducing different quality measures enabling the detailed comparison of competing approaches from different aspects. Furthermore, we set up a synthetic, computer generated benchmark providing a versatile tool for testing, with a couple of tunable parameters capable of generating a wide range of test beds. Beside the computer generated input we also use real data in our studies, including a biological example with a pre-defined hierarchy between the tags. The encouraging similarity between the pre-defined and reconstructed hierarchy, as well as the seemingly meaningful hierarchies obtained for other real systems indicate that tag hierarchy extraction is a very promising direction for further research with a great potential for practical applications. Tags have become very prevalent nowadays in various online platforms ranging from blogs through scientific publications to protein databases. Furthermore, tagging systems dedicated for voluntary tagging of photos, films, books, etc. with free words are also becoming popular. The emerging large collections of tags associated with different objects are often referred to as folksonomies, highlighting their collaborative origin and the "flat" organization of the tags opposed to traditional hierarchical categorization. Adding a tag hierarchy corresponding to a given folksonomy can very effectively help narrowing or broadening the scope of search. Moreover

  5. Extracting Tag Hierarchies

    PubMed Central

    Tibély, Gergely; Pollner, Péter; Vicsek, Tamás; Palla, Gergely

    2013-01-01

    Tagging items with descriptive annotations or keywords is a very natural way to compress and highlight information about the properties of the given entity. Over the years several methods have been proposed for extracting a hierarchy between the tags for systems with a "flat", egalitarian organization of the tags, which is very common when the tags correspond to free words given by numerous independent people. Here we present a complete framework for automated tag hierarchy extraction based on tag occurrence statistics. Along with proposing new algorithms, we are also introducing different quality measures enabling the detailed comparison of competing approaches from different aspects. Furthermore, we set up a synthetic, computer generated benchmark providing a versatile tool for testing, with a couple of tunable parameters capable of generating a wide range of test beds. Beside the computer generated input we also use real data in our studies, including a biological example with a pre-defined hierarchy between the tags. The encouraging similarity between the pre-defined and reconstructed hierarchy, as well as the seemingly meaningful hierarchies obtained for other real systems indicate that tag hierarchy extraction is a very promising direction for further research with a great potential for practical applications. Tags have become very prevalent nowadays in various online platforms ranging from blogs through scientific publications to protein databases. Furthermore, tagging systems dedicated for voluntary tagging of photos, films, books, etc. with free words are also becoming popular. The emerging large collections of tags associated with different objects are often referred to as folksonomies, highlighting their collaborative origin and the “flat” organization of the tags opposed to traditional hierarchical categorization. Adding a tag hierarchy corresponding to a given folksonomy can very effectively help narrowing or broadening the scope of search

  6. A tag-based approach for high-throughput analysis of CCWGG methylation.

    PubMed

    Denisova, Oksana V; Chernov, Andrei V; Koledachkina, Tatyana Y; Matvienko, Nicholas I

    2007-10-15

    Non-CpG methylation occurring in the context of CNG sequences is found in plants at a large number of genomic loci. However, there is still little information available about non-CpG methylation in mammals. Efficient methods that would allow detection of scarcely localized methylated sites in small quantities of DNA are required to elucidate the biological role of non-CpG methylation in both plants and animals. In this study, we tested a new whole genome approach to identify sites of CCWGG methylation (W is A or T), a particular case of CNG methylation, in genomic DNA. This technique is based on digestion of DNAs with methylation-sensitive restriction endonucleases EcoRII-C and AjnI. Short DNAs flanking methylated CCWGG sites (tags) are selectively purified and assembled in tandem arrays of up to nine tags. This allows high-throughput sequencing of tags, identification of flanking regions, and their exact positions in the genome. In this study, we tested specificity and efficiency of the approach.

  7. Launch of Space Shuttle Atlantis / STS-129 Mission

    NASA Image and Video Library

    2009-11-16

    CAPE CANAVERAL, Fla. - Twitter followers and media representatives at the NASA Press Site witness space shuttle Atlantis cut its way through the blue skies over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Gianni Woods

  8. Launch of Space Shuttle Atlantis / STS-129 Mission

    NASA Image and Video Library

    2009-11-16

    CAPE CANAVERAL, Fla. - Twitter followers and media representatives at the NASA Press Site watch as space shuttle Atlantis springs into action from Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Gianni Woods

  9. Launch of Space Shuttle Atlantis / STS-129 Mission

    NASA Image and Video Library

    2009-11-16

    CAPE CANAVERAL, Fla. - Twitter followers and media representatives at the NASA Press Site have front-row seats as space shuttle Atlantis launches through the clouds from Launch Pad 39A on a balmy Florida afternoon at NASA's Kennedy Space Center. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Gianni Woods

  10. Joint JSC/GSFC two-TDRS navigation certification results for STS-29, STS-30, and STS-32

    NASA Technical Reports Server (NTRS)

    Schmidt, Thomas G.; Brown, Edward T.; Murdock, Valerie E.; Cappellari, James O., Jr.; Smith, Evan A.; Schmitt, Mark W.; Omalley, James W.; Lowes, Flora B.; Joyce, James B.

    1990-01-01

    The procedures used and the results obtained in the joint Johnson Space Center (JSC)/Goddard Space Flight Center (GSFC) navigation certification of the two-Tracking and Data Relay Satellite (TDRS) S-band tracking configuration for support of low- to medium-inclination (28.5 to 62 degrees) Shuttle missions (STS-29 and STS-30) and Shuttle rendezvous missions (STS-32) are described. The objective of this certification effort was to certify the two-TDRS configuration for nominal Space Transportation System (STS) on-orbit navigation support, thereby making it possible to significantly reduce the ground tracking support requirements for routine STS on-orbit navigation. JSC had the primary responsibility for certification of the two-TDRS configuration for STS support, and GSFC supported the effort by performing Ground Network (GN) and Space Network (SN) tracking data evaluation, parallel orbit solutions, and solution comparisons. In the certification process, two types of orbit determination solutions were generated by JSC and by GSFC for each tracking arc evaluated, one type using TDRS-East and TDRS-West tracking data combined with ground tracking data (the reference solutions) and one type using only TDRS-East and TDRS-West tracking data. The two types of solutions were then compared to determine the maximum position differences over the solution arcs and whether these differences satisfied the navigation certification criteria. The certification criteria were a function of the type of Shuttle activity in the tracking arc, i.e., quiet, moderate, or active. Quiet periods included no attitude maneuvers or ventings; moderate periods included one or two maneuvers or ventings; and active periods included more than two maneuvers or ventings. The results of the individual JSC and GSFC certification analyses for the STS-29, STS-30, and STS-32 missions and the joint JSC/GSFC conclusions regarding certification of the two-TDRS S-band configuration for STS support are presented.

  11. Shark Tagging Activities.

    ERIC Educational Resources Information Center

    Current: The Journal of Marine Education, 1998

    1998-01-01

    In this group activity, children learn about the purpose of tagging and how scientists tag a shark. Using a cut-out of a shark, students identify, measure, record data, read coordinates, and tag a shark. Includes introductory information about the purpose of tagging and the procedure, a data sheet showing original tagging data from Tampa Bay, and…

  12. STS 62 Final Report

    NASA Technical Reports Server (NTRS)

    Rice, James E.

    1996-01-01

    The report is organized into sections representing the phases of work performed in analyzing the STS 62 results and preparing the instrument for STS 65. Section 2 contains a brief analysis of the STS 62 data which verifies the instrument changes to resolve the STS 58 problems. Section 3 describes the results from STS 62, including the original mission plan and several minor calibration period anomalies first discovered on STS 58 and reappearing to a lesser extent on STS 62.

  13. TSSi--an R package for transcription start site identification from 5' mRNA tag data.

    PubMed

    Kreutz, C; Gehring, J S; Lang, D; Reski, R; Timmer, J; Rensing, S A

    2012-06-15

    High-throughput sequencing has become an essential experimental approach for the investigation of transcriptional mechanisms. For some applications like ChIP-seq, several approaches for the prediction of peak locations exist. However, these methods are not designed for the identification of transcription start sites (TSSs) because such datasets contain qualitatively different noise. In this application note, the R package TSSi is presented which provides a heuristic framework for the identification of TSSs based on 5' mRNA tag data. Probabilistic assumptions for the distribution of the data, i.e. for the observed positions of the mapped reads, as well as for systematic errors, i.e. for reads which map closely but not exactly to a real TSS, are made and can be adapted by the user. The framework also comprises a regularization procedure which can be applied as a preprocessing step to decrease the noise and thereby reduce the number of false predictions. The R package TSSi is available from the Bioconductor web site: www.bioconductor.org/packages/release/bioc/html/TSSi.html.

  14. STS-132/ULF4 WFCR Flight Controllers on Console

    NASA Image and Video Library

    2010-05-14

    JSC2010-E-080432 (14 May 2010) --- Astronaut Charles Hobaugh, spacecraft communicator (CAPCOM) for the STS-132 mission, is pictured in the space shuttle flight control room in the Johnson Space Center's Mission Control Center during launch countdown activities a few hundred miles away in Florida, site of space shuttle Atlantis? STS-132 launch. Liftoff was on time at 2:20 p.m. (EDT) on May 14, 2010 from launch pad 39A at NASA's Kennedy Space Center.

  15. Comparative Performance of Acoustic-tagged and PIT-tagged Juvenile Salmonids

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hockersmith, Eric E.; Brown, Richard S.; Liedtke, Theresa L.

    2008-02-01

    Numerous research tools and technologies are currently being used to evaluate fish passage and survival to determine the impacts of the Federal Columbia River Power System (FCRPS) on endangered and threatened juvenile salmonids, including PIT tags, balloon tags, hydroacoustic evaluations, radio telemetry, and acoustic telemetry. Each has advantages and disadvantages, but options are restricted in some situations because of limited capabilities of a specific technology, lack of detection capability downstream, or availability of adequate numbers of fish. However, there remains concern about the comparative effects of the tag or the tagging procedure on fish performance. The recently developed Juvenile Salmonidmore » Acoustic Telemetry System (JSATS) acoustic transmitter is the smallest active acoustic tag currently available. The goal of this study was to determine whether fish tagged with the JSATS acoustic-telemetry tag can provide unbiased estimates of passage behavior and survival within the performance life of the tag. We conducted both field and laboratory studies to assess tag effects. For the field evaluation we released a total of 996 acoustic-tagged fish in conjunction with 21,026 PIT-tagged fish into the tailrace of Lower Granite Dam on 6 and 13 May. Travel times between release and downstream dams were not significantly different for the majority of the reaches between acoustic-tagged and PIT-tagged fish. In addition to the field evaluation, a series of laboratory experiments were conducted to determine if growth and survival of juvenile Chinook salmon surgically implanted with acoustic transmitters is different than untagged or PIT tagged juvenile Chinook salmon. Only yearling fish with integrated and non-integrated transmitters experienced mortalities, and these were low (<4.5%). Mortality among sub-yearling control and PIT-tag treatments ranged up to 7.7% while integrated and non-integrated treatments had slightly higher rates (up to 8.3% and 7

  16. 40 CFR 35.4045 - What requirements must my group meet as a TAG recipient?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... for the purpose of participating in decision making at the Superfund site for which we provide a TAG... 40 Protection of Environment 1 2010-07-01 2010-07-01 false What requirements must my group meet as... Responsibilities As A Tag Recipient § 35.4045 What requirements must my group meet as a TAG recipient? Your group...

  17. Ontologies and tag-statistics

    NASA Astrophysics Data System (ADS)

    Tibély, Gergely; Pollner, Péter; Vicsek, Tamás; Palla, Gergely

    2012-05-01

    Due to the increasing popularity of collaborative tagging systems, the research on tagged networks, hypergraphs, ontologies, folksonomies and other related concepts is becoming an important interdisciplinary area with great potential and relevance for practical applications. In most collaborative tagging systems the tagging by the users is completely ‘flat’, while in some cases they are allowed to define a shallow hierarchy for their own tags. However, usually no overall hierarchical organization of the tags is given, and one of the interesting challenges of this area is to provide an algorithm generating the ontology of the tags from the available data. In contrast, there are also other types of tagged networks available for research, where the tags are already organized into a directed acyclic graph (DAG), encapsulating the ‘is a sub-category of’ type of hierarchy between each other. In this paper, we study how this DAG affects the statistical distribution of tags on the nodes marked by the tags in various real networks. The motivation for this research was the fact that understanding the tagging based on a known hierarchy can help in revealing the hidden hierarchy of tags in collaborative tagging systems. We analyse the relation between the tag-frequency and the position of the tag in the DAG in two large sub-networks of the English Wikipedia and a protein-protein interaction network. We also study the tag co-occurrence statistics by introducing a two-dimensional (2D) tag-distance distribution preserving both the difference in the levels and the absolute distance in the DAG for the co-occurring pairs of tags. Our most interesting finding is that the local relevance of tags in the DAG (i.e. their rank or significance as characterized by, e.g., the length of the branches starting from them) is much more important than their global distance from the root. Furthermore, we also introduce a simple tagging model based on random walks on the DAG, capable of

  18. STS 63: Post flight presentation

    NASA Astrophysics Data System (ADS)

    1995-02-01

    At a post flight conference, Captain Jim Wetherbee, of STS Flight 63, introduces each of the other members of the STS 63 crew (Eileen Collins, Pilot; Dr. Bernard Harris, Payload Commander; Dr. Michael Foale, Mission Specialist from England; Dr. Janice Voss, Mission Specialist; and Colonel Vladimir Titor, Mission Specialist from Russia), gave a short autobiography of each member and a brief description of their assignment during this mission. A film was shown that included the preflight suit-up, a view of the launch site, the actual night launch, a tour of the Space Shuttle and several of the experiment areas, several views of earth and the MIR Space Station and cosmonauts, the MlR-Space Shuttle rendezvous, the deployment of the Spartan Ultraviolet Telescope, Foale and Harris's EVA and space walk, the retrieval of Spartan, and the night entry home, including the landing. Several spaceborne experiments were introduced: the radiation monitoring experiment, environment monitoring experiment, solid surface combustion experiment, and protein crystal growth and plant growth experiments. This conference ended with still, color pictures, taken by the astronauts during the entire STS 63 flight, being shown.

  19. STS 63: Post Flight Presentation

    NASA Technical Reports Server (NTRS)

    1995-01-01

    At a post flight conference, Captain Jim Wetherbee, of STS Flight 63, introduces each of the other members of the STS 63 crew (Eileen Collins, Pilot; Dr. Bernard Harris, Payload Commander; Dr. Michael Foale, Mission Specialist from England; Dr. Janice Voss, Misssion Specialist; and Colonel Vladimir Titor, Misssion Specialist from Russia. A short biography of each member and a brief description of their assignment during this mission is given. A film was shown that included the preflight suit-up, a view of the launch site, the actual night launch, a tour of the Space Shuttle and several of the experiment areas, several views of earth and the MIR Space Station and cosmonauts, the MIR-Space Shuttle rendezvous, the deployment of the Spartan Ultraviolet Telescope, Foale and Harris's EVA and space walk, the retrieval of Spartan, and the night entry home, including the landing. Several spaceborne experiments were introduced: the radiation monitoring experiment, environment monitoring experiment, solid surface combustion experiment, and protein crystal growth and plant growth experiments. This conference ended with still, color pictures, taken by the astronauts during the entire STS 63 flight, being shown.

  20. STS-132/ULF4 WFCR Flight Controllers on Console

    NASA Image and Video Library

    2010-05-14

    JSC2010-E-080410 (14 May 2010) --- Astronauts Steve Frick (standing) and Charles Hobaugh, both spacecraft communicators (CAPCOM) for the STS-132 mission, are pictured in the space shuttle flight control room in the Johnson Space Center's Mission Control Center during launch countdown activities a few hundred miles away in Florida, site of space shuttle Atlantis? STS-132 launch. Liftoff was on time at 2:20 p.m. (EDT) on May 14, 2010 from launch pad 39A at NASA's Kennedy Space Center.

  1. STS-120 and Expedition 16 Commanders in Node 1/Unity module

    NASA Image and Video Library

    2007-11-04

    S120-E-008350 (4 Nov. 2007) --- Astronauts Pam Melroy (foreground), STS-120 commander; and Peggy Whitson, Expedition 16 commander, add the STS-120 crew patch in the Unity node to the growing collection of those representing shuttle crews who have worked on the International Space Station. A location in the Unity node serves as one of the traditional posting sites for the patches.

  2. Cross-species amplification of mitochondrial DNA sequence-tagged-site markers in conifers: the nature of polymorphism and variation within and among species in Picea.

    PubMed

    Jaramillo-Correa, J P; Bousquet, J; Beaulieu, J; Isabel, N; Perron, M; Bouillé, M

    2003-05-01

    Primers previously developed to amplify specific non-coding regions of the mitochondrial genome in Angiosperms, and new primers for additional non-coding mtDNA regions, were tested for their ability to direct DNA amplification in 12 conifer taxa and to detect sequence-tagged-site (STS) polymorphisms within and among eight species in Picea. Out of 12 primer pairs, nine were successful at amplifying mtDNA in most of the taxa surveyed. In conifers, indels and substitutions were observed for several loci, allowing them to distinguish between families, genera and, in some cases, between species within genera. In Picea, interspecific polymorphism was detected for four loci, while intraspecific variation was observed for three of the mtDNA regions studied. One of these (SSU rRNA V1 region) exhibited indel polymorphisms, and the two others ( nad1 intron b/c and nad5 intron1) revealed restriction differences after digestion with Sau3AI (PCR-RFLP). A fourth locus, the nad4L- orf25 intergenic region, showed a multibanding pattern for most of the spruce species, suggesting a possible gene duplication. Maternal inheritance, expected for mtDNA in conifers, was observed for all polymorphic markers except the intergenic region nad4L- orf25. Pooling of the variation observed with the remaining three markers resulted in two to six different mtDNA haplotypes within the different species of Picea. Evidence for intra-genomic recombination was observed in at least two taxa. Thus, these mitotypes are likely to be more informative than single-locus haplotypes. They should be particularly useful for the study of biogeography and the dynamics of hybrid zones.

  3. Dynamic optical tags

    NASA Astrophysics Data System (ADS)

    Griggs, Steven P.; Mark, Martin B.; Feldman, Barry J.

    2004-07-01

    The goal of the DARPA Dynamic Optical Tags (DOTs) program is to develop a small, robust, persistent, 2-way tagging, tracking and locating device that also supports communications at data rates greater than 100 kbps and can be interrogated at significant range. These tags will allow for two-way data exchange and tagging operations in friendly and denied areas. The DOTs will be passive and non-RF. To accomplish this, the DOTs program will develop small, thin, retro-reflecting modulators. The tags will operate for long periods of time (greater than two months) in real-world environmental conditions (-40° to +70° C) and allow for a wide interrogation angle (+/-60°). The tags will be passive (in the sleep mode) for most of the time and only become active when interrogated by a laser with the correct code. Once correctly interrogated, the tags will begin to modulate and retro-reflect the incoming beam. The program will also develop two tag specific transceiver systems that are eye-safe, employ automated scanning algorithms, and are capable of short search and interrogate times.

  4. Separation efficiency of free-solution conjugated electrophoresis with drag-tags incorporating a synthetic amino acid.

    PubMed

    Seo, Kyung-Ho; Chu, Hun-Su; Yoo, Tae Hyeon; Lee, Sun-Gu; Won, Jong-In

    2016-03-01

    DNA sequencing or separation by conventional capillary electrophoresis with a polymer matrix has some inherent drawbacks, such as the expense of polymer matrix and limitations in sequencing read length. As DNA fragments have a linear charge-to-friction ratio in free solution, DNA fragments cannot be separated by size. However, size-based separation of DNA is possible in free-solution conjugate electrophoresis (FSCE) if a "drag-tag" is attached to DNA fragments because the tag breaks the linear charge-to-friction scaling. Although several previous studies have demonstrated the feasibility of DNA separation by free-solution conjugated electrophoresis, generation of a monodisperse drag-tag and identification of a strong, site-specific conjugation method between a DNA fragment and a drag-tag are challenges that still remain. In this study, we demonstrate an efficient FSCE method by conjugating a biologically synthesized elastin-like polypeptide (ELP) and green fluorescent protein (GFP) to DNA fragments. In addition, to produce strong and site-specific conjugation, a methionine residue in drag-tags is replaced with homopropargylglycine (Hpg), which can be conjugated specifically to a DNA fragment with an azide site. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. A laboratory evaluation of tagging-related mortality and tag loss in juvenile humpback chub

    USGS Publications Warehouse

    Ward, David L.; Persons, William R.; Young, Kirk; Stone, Dennis M.; Van Haverbeke, Randy; Knight, William R.

    2015-01-01

    We quantified tag retention, survival, and growth in juvenile, captive-reared Humpback Chub Gila cypha marked with three different tag types: (1) Biomark 12.5-mm, 134.2-kHz, full duplex PIT tags injected into the body cavity with a 12-gauge needle; (2) Biomark 8.4-mm, 134.2-kHz, full duplex PIT tags injected with a 16-gauge needle; and (3) Northwest Marine Technology visible implant elastomer (VIE) tags injected under the skin with a 29-gauge needle. Estimates of tag loss, tagging-induced mortality, and growth were evaluated for 60 d with each tag type for four different size-groups of fish: 40–49 mm, 50–59 mm, 60–69 mm, and 70–79 mm TL. Total length was a significant predictor of the probability of PIT tag retention and mortality for both 8-mm and 12-mm PIT tags, and the smallest fish had the highest rates of tag loss (12.5–30.0%) and mortality (7.5–20.0%). Humpback Chub of sizes 40–49 mm TL and tagged with VIE tags had no mortality but did have a 17.5% tag loss. Growth rates of all tagged fish were similar to controls. Our data indicate Humpback Chub can be effectively tagged using either 8-mm or 12-mm PIT tags with little tag loss or mortality at sizes as low as 65 mm TL.

  6. Tagging insulin in microgravity

    NASA Technical Reports Server (NTRS)

    Dobeck, Michael; Nelson, Ronald S.

    1992-01-01

    Knowing the exact subcellular sites of action of insulin in the body has the potential to give basic science investigators a basis from which a cause and cure for this disease can be approached. The goal of this project is to create a test reagent that can be used to visualize these subcellular sites. The unique microgravity environment of the Shuttle will allow the creation of a reagent that has the possibility of elucidating the subcellular sites of action of insulin. Several techniques have been used in an attempt to isolate the sites of action of items such as insulin. One of these is autoradiography in which the test item is obtained from animals fed radioactive materials. What is clearly needed is to visualize individual insulin molecules at their sites of action. The insulin tagging process to be used on G-399 involves the conjugation of insulin molecules with ferritin molecules to create a reagent that will be used back on Earth in an attempt to elucidate the sites of action of insulin.

  7. Site-Specifically Labeled Immunoconjugates for Molecular Imaging--Part 2: Peptide Tags and Unnatural Amino Acids.

    PubMed

    Adumeau, Pierre; Sharma, Sai Kiran; Brent, Colleen; Zeglis, Brian M

    2016-04-01

    Molecular imaging using radioisotope- or fluorophore-labeled antibodies is increasingly becoming a critical component of modern precision medicine. Yet despite this promise, the vast majority of these immunoconjugates are synthesized via the random coupling of amine-reactive bifunctional probes to lysines within the antibody, a process that can result in heterogeneous and poorly defined constructs with suboptimal pharmacological properties. In an effort to circumvent these issues, the last 5 years have played witness to a great deal of research focused on the creation of effective strategies for the site-specific attachment of payloads to antibodies. These chemoselective modification methods yield immunoconjugates that are more homogenous and better defined than constructs created using traditional synthetic approaches. Moreover, site-specifically labeled immunoconjugates have also been shown to exhibit superior in vivo behavior compared to their randomly modified cousins. The over-arching goal of this two-part review is to provide a broad yet detailed account of the various site-specific bioconjugation approaches that have been used to create immunoconjugates for positron emission tomography (PET), single photon emission computed tomography (SPECT), and fluorescence imaging. In Part 1, we covered site-specific bioconjugation techniques based on the modification of cysteine residues and the chemoenzymatic manipulation of glycans. In Part 2, we will detail two families of bioconjugation approaches that leverage biochemical tools to achieve site-specificity. First, we will discuss modification methods that employ peptide tags either as sites for enzyme-catalyzed ligations or as radiometal coordination architectures. And second, we will examine bioconjugation strategies predicated on the incorporation of unnatural or non-canonical amino acids into antibodies via genetic engineering. Finally, we will compare the advantages and disadvantages of the modification

  8. STS-135 Atlantis Launch

    NASA Image and Video Library

    2011-07-07

    NASA Administrator Charles Bolden speaks to visitors at the NASA Kennedy Space Center Banana Creek viewing site prior to going to the Launch Control Center (LCC) for the planned launch of the space shuttle Atlantis from pad 39A on Friday, July 8, 2011, in Cape Canaveral, Fla. The launch of Atlantis, STS-135, is the final flight of the shuttle program, a 12-day mission to the International Space Station. Photo Credit: (NASA/Bill Ingalls)

  9. Hypergraph topological quantities for tagged social networks.

    PubMed

    Zlatić, Vinko; Ghoshal, Gourab; Caldarelli, Guido

    2009-09-01

    Recent years have witnessed the emergence of a new class of social networks, which require us to move beyond previously employed representations of complex graph structures. A notable example is that of the folksonomy, an online process where users collaboratively employ tags to resources to impart structure to an otherwise undifferentiated database. In a recent paper, we proposed a mathematical model that represents these structures as tripartite hypergraphs and defined basic topological quantities of interest. In this paper, we extend our model by defining additional quantities such as edge distributions, vertex similarity and correlations as well as clustering. We then empirically measure these quantities on two real life folksonomies, the popular online photo sharing site Flickr and the bookmarking site CiteULike. We find that these systems share similar qualitative features with the majority of complex networks that have been previously studied. We propose that the quantities and methodology described here can be used as a standard tool in measuring the structure of tagged networks.

  10. Hypergraph topological quantities for tagged social networks

    NASA Astrophysics Data System (ADS)

    Zlatić, Vinko; Ghoshal, Gourab; Caldarelli, Guido

    2009-09-01

    Recent years have witnessed the emergence of a new class of social networks, which require us to move beyond previously employed representations of complex graph structures. A notable example is that of the folksonomy, an online process where users collaboratively employ tags to resources to impart structure to an otherwise undifferentiated database. In a recent paper, we proposed a mathematical model that represents these structures as tripartite hypergraphs and defined basic topological quantities of interest. In this paper, we extend our model by defining additional quantities such as edge distributions, vertex similarity and correlations as well as clustering. We then empirically measure these quantities on two real life folksonomies, the popular online photo sharing site Flickr and the bookmarking site CiteULike. We find that these systems share similar qualitative features with the majority of complex networks that have been previously studied. We propose that the quantities and methodology described here can be used as a standard tool in measuring the structure of tagged networks.

  11. Assessment of PIT tag retention and post-tagging survival in metamorphosing juvenile Sea Lamprey

    USGS Publications Warehouse

    Simard, Lee G.; Sotola, V. Alex; Marsden, J. Ellen; Miehls, Scott M.

    2017-01-01

    Background: Passive integrated transponder (PIT) tags have been used to document and monitor the movement or behavior of numerous species of fishes. Data on short-term and long-term survival and tag retention are needed before initiating studies using PIT tags on a new species or life stage. We evaluated the survival and tag retention of 153 metamorphosing juvenile Sea Lamprey Petromyzon marinus tagged with 12 mm PIT tags on three occasions using a simple surgical procedure. Results: Tag retention was 100% and 98.6% at 24 h and 28-105 d post-tagging. Of the lamprey that retained their tags, 87.3% had incisions sufficiently healed to prevent further loss. Survival was 100% and 92.7% at 24 h and 41-118 d post-tagging with no significant difference in survival between tagged and untagged control lamprey. Of the 11 lamprey that died, four had symptoms that indicated their death was directly related to tagging. Survival was positively correlated with Sea Lamprey length. Conclusions: Given the overall high level of survival and tag retention in this study, future studies can utilize 12 mm PIT tags to monitor metamorphosing juvenile Sea Lamprey movement and migration patterns.

  12. Biotin-tagged proteins: Reagents for efficient ELISA-based serodiagnosis and phage display-based affinity selection

    PubMed Central

    Verma, Vaishali; Kaur, Charanpreet; Grover, Payal; Gupta, Amita

    2018-01-01

    The high-affinity interaction between biotin and streptavidin has opened avenues for using recombinant proteins with site-specific biotinylation to achieve efficient and directional immobilization. The site-specific biotinylation of proteins carrying a 15 amino acid long Biotin Acceptor Peptide tag (BAP; also known as AviTag) is effected on a specific lysine either by co-expressing the E. coli BirA enzyme in vivo or by using purified recombinant E. coli BirA enzyme in the presence of ATP and biotin in vitro. In this paper, we have designed a T7 promoter-lac operator-based expression vector for rapid and efficient cloning, and high-level cytosolic expression of proteins carrying a C-terminal BAP tag in E. coli with TEV protease cleavable N-terminal deca-histidine tag, useful for initial purification. Furthermore, a robust three-step purification pipeline integrated with well-optimized protocols for TEV protease-based H10 tag removal, and recombinant BirA enzyme-based site-specific in vitro biotinylation is described to obtain highly pure biotinylated proteins. Most importantly, the paper demonstrates superior sensitivities in indirect ELISA with directional and efficient immobilization of biotin-tagged proteins on streptavidin-coated surfaces in comparison to passive immobilization. The use of biotin-tagged proteins through specific immobilization also allows more efficient selection of binders from a phage-displayed naïve antibody library. In addition, for both these applications, specific immobilization requires much less amount of protein as compared to passive immobilization and can be easily multiplexed. The simplified strategy described here for the production of highly pure biotin-tagged proteins will find use in numerous applications, including those, which may require immobilization of multiple proteins simultaneously on a solid surface. PMID:29360877

  13. Biotin-tagged proteins: Reagents for efficient ELISA-based serodiagnosis and phage display-based affinity selection.

    PubMed

    Verma, Vaishali; Kaur, Charanpreet; Grover, Payal; Gupta, Amita; Chaudhary, Vijay K

    2018-01-01

    The high-affinity interaction between biotin and streptavidin has opened avenues for using recombinant proteins with site-specific biotinylation to achieve efficient and directional immobilization. The site-specific biotinylation of proteins carrying a 15 amino acid long Biotin Acceptor Peptide tag (BAP; also known as AviTag) is effected on a specific lysine either by co-expressing the E. coli BirA enzyme in vivo or by using purified recombinant E. coli BirA enzyme in the presence of ATP and biotin in vitro. In this paper, we have designed a T7 promoter-lac operator-based expression vector for rapid and efficient cloning, and high-level cytosolic expression of proteins carrying a C-terminal BAP tag in E. coli with TEV protease cleavable N-terminal deca-histidine tag, useful for initial purification. Furthermore, a robust three-step purification pipeline integrated with well-optimized protocols for TEV protease-based H10 tag removal, and recombinant BirA enzyme-based site-specific in vitro biotinylation is described to obtain highly pure biotinylated proteins. Most importantly, the paper demonstrates superior sensitivities in indirect ELISA with directional and efficient immobilization of biotin-tagged proteins on streptavidin-coated surfaces in comparison to passive immobilization. The use of biotin-tagged proteins through specific immobilization also allows more efficient selection of binders from a phage-displayed naïve antibody library. In addition, for both these applications, specific immobilization requires much less amount of protein as compared to passive immobilization and can be easily multiplexed. The simplified strategy described here for the production of highly pure biotin-tagged proteins will find use in numerous applications, including those, which may require immobilization of multiple proteins simultaneously on a solid surface.

  14. PIT Tagging Anurans

    USGS Publications Warehouse

    McCreary, Brome

    2008-01-01

    The following video demonstrates a procedure to insert a passive integrated transponder (PIT) tag under the skin of an anuran (frog or toad) for research and monitoring purposes. Typically, a 12.5 mm tag (0.5 in.) is used to uniquely identify individual anurans as smal as 40 mm (1.6 in.) in length from snout to vent. Smaller tags are also available and allow smaller anurans to be tagged. The procedure does not differ for other sizes of tages or other sizes of anurans. Anyone using this procedure should ensure that the tag is small enough to fit easily behind the sacral hump of the anuran, as shown in this video.

  15. Development of RAP Tag, a Novel Tagging System for Protein Detection and Purification.

    PubMed

    Fujii, Yuki; Kaneko, Mika K; Ogasawara, Satoshi; Yamada, Shinji; Yanaka, Miyuki; Nakamura, Takuro; Saidoh, Noriko; Yoshida, Kanae; Honma, Ryusuke; Kato, Yukinari

    2017-04-01

    Affinity tag systems, possessing high affinity and specificity, are useful for protein detection and purification. The most suitable tag for a particular purpose should be selected from many available affinity tag systems. In this study, we developed a novel affinity tag called the "RAP tag" system, which comprises a mouse antirat podoplanin monoclonal antibody (clone PMab-2) and the RAP tag (DMVNPGLEDRIE). This system is useful not only for protein detection in Western blotting, flow cytometry, and sandwich enzyme-linked immunosorbent assay, but also for protein purification.

  16. ezTag: tagging biomedical concepts via interactive learning.

    PubMed

    Kwon, Dongseop; Kim, Sun; Wei, Chih-Hsuan; Leaman, Robert; Lu, Zhiyong

    2018-05-18

    Recently, advanced text-mining techniques have been shown to speed up manual data curation by providing human annotators with automated pre-annotations generated by rules or machine learning models. Due to the limited training data available, however, current annotation systems primarily focus only on common concept types such as genes or diseases. To support annotating a wide variety of biological concepts with or without pre-existing training data, we developed ezTag, a web-based annotation tool that allows curators to perform annotation and provide training data with humans in the loop. ezTag supports both abstracts in PubMed and full-text articles in PubMed Central. It also provides lexicon-based concept tagging as well as the state-of-the-art pre-trained taggers such as TaggerOne, GNormPlus and tmVar. ezTag is freely available at http://eztag.bioqrator.org.

  17. Connectivity clues from short-term variability in settlement and geochemical tags of mytilid mussels

    NASA Astrophysics Data System (ADS)

    Fodrie, F. Joel; Becker, Bonnie J.; Levin, Lisa A.; Gruenthal, Kristen; McMillan, Pat A.

    2011-01-01

    The use of geochemical tags in calcified structures of fish and invertebrates is an exciting tool for investigating larval population connectivity. Tag evaluation over relatively short intervals (weeks) may detect environmental and ecological variability at a temporal scale highly relevant to larval transport and settlement. We collected newly settled mussels ( Mytilus californianus and M. galloprovincialis) weekly during winter/spring of 2002 along the coast of San Diego, CA, USA, at sites on the exposed coast (SIO) and in a protected coastal bay (HI), to investigate temporal patterns of geochemical tags in mussel shells. Analyses of post-settlement shell via LA-ICP-MS revealed statistically significant temporal variability for all elements we examined (Mg, Mn, Cu, Sr, Cd, Ba, Pb and U). Despite this, our ability to distinguish multielemental signatures between sites was largely conserved. Throughout our 13-week study, SIO and HI mussels could be chemically distinguished from one another in 78-87% of all cases. Settlement varied between 2 and 27 settlers gram-byssus -1 week -1 at SIO and HI, and both sites were characterized by 2-3 weeks with "high" settlement. Geochemical tags recorded in early larval shell of newly settled mussels differed between "high" and "low" settlement weeks at both sites (MANOVA), driven by Mg and Sr at SIO (p = 0.013) and Sr, Cd, Ba and Pb at HI (p < 0.001). These data imply that shifts in larval sources or transport corridors were responsible for observed settlement variation, rather than increased larval production. In particular, increased settlement at HI was observed concurrent with the appearance of geochemical tags (e.g., elevated Cd), suggesting that those larvae were retained in upwelled water near the mouth of the bay. Such shifts may reflect short-term changes in connectivity among sites due to altered transport corridors, and influence the demography of local populations.

  18. Cutaneous skin tag

    MedlinePlus

    Skin tag; Acrochordon; Fibroepithelial polyp ... have diabetes. They are thought to occur from skin rubbing against skin. ... The tag sticks out of the skin and may have a short, narrow stalk connecting it to the surface of the skin. Some skin tags are as long as ...

  19. STS-34: Mission Overview Briefing

    NASA Technical Reports Server (NTRS)

    1989-01-01

    Live footage shows Milt Heflin, the Lead Flight Director participating in the STS-34 Mission Briefing. He addresses the primary objective, and answered questions from the audience and other NASA Centers. Heflin also mentions the Shuttle Solar Backscatter Ultraviolet secondary payload, and several experiments. These experiments include Growth Hormone Crystal Distribution (Plants), Polymer Morphology, Sensor Technology Experiment, Mesoscale Lightning Experiment, Shuttle Student Involvement Program "Ice Crystals", and the Air Force Maui Optical Site.

  20. Launch of STS-66 Space Shuttle Atlantis

    NASA Technical Reports Server (NTRS)

    1994-01-01

    The Space Shuttle Atlantis returns to work after a refurbishing and a two-year layoff, as liftoff for NASA's STS-66 occurs at noon (EDT), November 3, 1994. A 35mm camera was used to record the image, which includes much of the base of the launch site as well as the launch itself.

  1. A Modular Vaccine Development Platform Based on Sortase-Mediated Site-Specific Tagging of Antigens onto Virus-Like Particles

    PubMed Central

    Tang, Shubing; Xuan, Baoqin; Ye, Xiaohua; Huang, Zhong; Qian, Zhikang

    2016-01-01

    Virus-like particles (VLPs) can be used as powerful nanoscale weapons to fight against virus infection. In addition to direct use as vaccines, VLPs have been extensively exploited as platforms on which to display foreign antigens for prophylactic vaccination and immunotherapeutic treatment. Unfortunately, fabrication of new chimeric VLP vaccines in a versatile, site-specific and highly efficient manner is beyond the capability of traditional VLP vaccine design approaches, genetic insertion and chemical conjugation. In this study, we described a greatly improved VLP display strategy by chemoenzymatic site-specific tailoring antigens on VLPs surface with high efficiency. Through the transpeptidation mediated by sortase A, one protein and two epitopes containing N-terminal oligoglycine were conjugated to the LPET motif on the surface of hepatitis B virus core protein (HBc) VLPs with high density. All of the new chimeric VLPs induced strong specific IgG responses. Furthermore, the chimeric VLPs with sortase A tagged enterovirus 71 (EV71) SP70 epitope could elicit effective antibodies against EV71 lethal challenging as well as the genetic insertion chimeric VLPs. The sortase A mediated chemoenzymatic site-specific tailoring of the HBc VLP approach shows great potential in new VLP vaccine design for its simplicity, site specificity, high efficiency, and versatility. PMID:27170066

  2. Lamprey Tagging

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Colotelo, Alison; Deters, Kate

    2017-05-26

    Pacific Northwest National Laboratory has developed a super-small acoustic tracking tag designed just for juvenile lamprey. In this video, PNNL researcher Alison Colotelo describes how she and her colleague Kate Deters inject young lamprey with the PNNL tag.

  3. Photon-tagged and B-meson-tagged b-jet production at the LHC

    DOE PAGES

    Huang, Jinrui; Kang, Zhong -Bo; Vitev, Ivan; ...

    2015-09-18

    Tagged jet measurements in high energy hadronic and nuclear reactions provide constraints on the energy and parton flavor origin of the parton shower that recoils against the tagging particle. Such additional insight can be especially beneficial in illuminating the mechanisms of heavy flavor production in proton–proton collisions at the LHC and their modification in the heavy ion environment, which are not fully understood. With this motivation, we present theoretical results for isolated-photon-tagged and B-meson-tagged b-jet production at √s NN = 5.1 TeV for comparison to the upcoming lead–lead data. We find that photon-tagged b-jets exhibit smaller momentum imbalance shift inmore » nuclear matter, and correspondingly smaller energy loss, than photon-tagged light flavor jets. Our results show that B-meson tagging is most effective in ensuring that the dominant fraction of recoiling jets originate from prompt b-quarks. Furthermore, in this channel the large suppression of the cross section is not accompanied by a significant momentum imbalance shift.« less

  4. STS-133 crew visit

    NASA Image and Video Library

    2011-04-20

    Stennis Space Center Deputy Director Rick Gilbrech (far right) welcomes members of the STS-133 shuttle mission crew during an April 20 visit. The mission was the final flight for the space shuttle Discovery, which now becomes the first of the three-orbiter fleet to be retired. During the visit to Stennis, Mission Commander Steven Lindsey ( l to r), Pilot Eric Boe and mission specialists Alvin Drew, Steven Bowen, Michael Barratt and Nicole Stott recapped their historic flight and thanked site employees for providing main engines that performed 'as advertised.'

  5. IIaO ultraviolet and nuclear emulsion films responses to orbital flights on STS-3, STS-7, STS-8, and STS-40

    NASA Technical Reports Server (NTRS)

    Hammond, E. C., Jr.; Peters, K. A.; Blake, S. M.; Bailey, Y.; Johnson, D.; Robancho, S.; Stober, A.

    1992-01-01

    Two types of film were flown on STS-40 space shuttle mission in June 1991. The IIaO special purpose ultraviolet film showed continued desensitization because of various thermal and cosmic ray interactions. The films were exposed to the space orbital environment for 9 days. There were several built-in launch pad delays of the shuttle mission. However, there was adequate monitoring of the temperature variations on board the shuttle that allowed for adequate knowledge of the thermal film history. This IIaO film was flown on the ASTRO I mission and is currently slated for use with the ASTRO II mission. A 50 micron thick IIIford Nuclear emulsion film was also placed on a 175 micron polyester base. The exposure to space produced several cosmic ray interactions that were analyzed and measured using Digital Image Processing techniques. This same nuclear emulsion film was flown on STS-8 and produced a similar number of cosmic ray and thermal interactions. From previous experiments of film using various laboratory electromagnetic radiation sources (e.g., alpha, beta, and neutron particles), we have been able to infer the possible oribtal interactions of both IIaO and nuclear emulsion films. The characteristic responses of IIaO on STS-40 compared favorably to the results obtained from previous STS-7 and STS-8 gas can experiments. The results indicate sufficient evidence correlating increased density on the film with possible cosmic ray, thermal and shuttle out gassing interactions.

  6. The impact of a dedicated Science-Technology-Society (STS) course on student knowledge of STS content

    NASA Astrophysics Data System (ADS)

    Barron, Paul E.

    In the last half century, public awareness of issues such as population growth, environmental pollution and the threat of nuclear war has pressured science education to reform to increase student social responsibility. The emerging Science-Technology-Society (STS) movement addressed these concerns by developing numerous strategies and curricula. Considerable diagnostic research has been conducted on student knowledge of the nature of science, but not on the wider scope of STS content (e.g., the nature of science and technology and their interactions with society). However, researchers have not widely studied the impact of comprehensive STS curricula on students' knowledge of STS content nor the nature of STS teaching practice that influenced this knowledge gain. This study examined student success and teacher performance in a special STS course in Ontario, Canada. Research questions focused on the STS content knowledge gain by students completing this course and the impact of the STS teachers' teaching practices on this knowledge gain. Student data were collected using pre-course and post-course assessments of students' STS content knowledge. Teacher data were obtained using semi-structured interviews, classroom observations and videotapes. Statistical analysis indicated that, after completing the STS course, students significantly increased their STS content knowledge as measured by the Views on Science Technology Society instrument. Gender and academic achievement had no significant impact on this knowledge gain, implying that this course, as taught by these teachers, could appeal to a wide range of students as a general education course. The second part of the study indicated that detailed research is needed on the relationship between STS teaching practice and student STS content knowledge gain. The small sample size prevents generalizations but initial indications show that factors such constructivist teaching practices and strong teacher STS content knowledge

  7. STS-63 Space Shuttle report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W., Jr.

    1995-01-01

    The STS-63 Space Shuttle Program Mission Report summarizes the Payload activities and provides detailed data on the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Reusable Solid Rocket Motor (RSRM), and the Space Shuttle Main Engine (SSME) systems performance during this sixty-seventh flight of the Space Shuttle Program, the forty-second since the return to flight, and twentieth flight of the Orbiter vehicle Discovery (OV-103). In addition to the OV-103 Orbiter vehicle, the flight vehicle consisted of an ET that was designated ET-68; three SSME's that were designated 2035, 2109, and 2029 in positions 1, 2, and 3, respectively; and two SRB's that were designated BI-070. The RSRM's that were an integral part of the SRB's were designated 360Q042A for the left SRB and 360L042B for the right SRB. The STS-63 mission was planned as an 8-day duration mission with two contingency days available for weather avoidance or Orbiter contingency operations. The primary objectives of the STS-63 mission were to perform the Mir rendezvous operations, accomplish the Spacehab-3 experiments, and deploy and retrieve the Shuttle Pointed Autonomous Research Tool for Astronomy-204 (SPARTAN-204) payload. The secondary objectives were to perform the Cryogenic Systems Experiment (CSE)/Shuttle Glo-2 Experiment (GLO-2) Payload (CGP)/Orbital Debris Radar Calibration Spheres (ODERACS-2) (CGP/ODERACS-2) payload objectives, the Solid Surface Combustion Experiment (SSCE), and the Air Force Maui Optical Site Calibration Tests (AMOS). The objectives of the Mir rendezvous/flyby were to verify flight techniques, communication and navigation-aid sensor interfaces, and engineering analyses associated with Shuttle/Mir proximity operations in preparation for the STS-71 docking mission.

  8. STS-135 New York City Visit

    NASA Image and Video Library

    2011-08-17

    New York Mayor Michael Bloomberg, center, talks about the construction around the site where the World Trade Center once stood to NASA Deputy Administrator Lori Garver, right, and members of the STS-135 crew including commander Chris Ferguson, left, mission specialist Rex Walheim, second right, and pilot Doug Hurley, rear, as they tour the area, Wednesday evening, Aug. 18, 2011, in New York. Photo Credit: (NASA/Paul E. Alers)

  9. Understanding why users tag: A survey of tagging motivation literature and results from an empirical study.

    PubMed

    Strohmaier, Markus; Körner, Christian; Kern, Roman

    2012-12-01

    While recent progress has been achieved in understanding the structure and dynamics of social tagging systems, we know little about the underlying user motivations for tagging, and how they influence resulting folksonomies and tags. This paper addresses three issues related to this question. (1) What distinctions of user motivations are identified by previous research, and in what ways are the motivations of users amenable to quantitative analysis? (2) To what extent does tagging motivation vary across different social tagging systems? (3) How does variability in user motivation influence resulting tags and folksonomies? In this paper, we present measures to detect whether a tagger is primarily motivated by categorizing or describing resources, and apply these measures to datasets from seven different tagging systems. Our results show that (a) users' motivation for tagging varies not only across, but also within tagging systems, and that (b) tag agreement among users who are motivated by categorizing resources is significantly lower than among users who are motivated by describing resources . Our findings are relevant for (1) the development of tag-based user interfaces, (2) the analysis of tag semantics and (3) the design of search algorithms for social tagging systems.

  10. Understanding why users tag: A survey of tagging motivation literature and results from an empirical study

    PubMed Central

    Strohmaier, Markus; Körner, Christian; Kern, Roman

    2012-01-01

    While recent progress has been achieved in understanding the structure and dynamics of social tagging systems, we know little about the underlying user motivations for tagging, and how they influence resulting folksonomies and tags. This paper addresses three issues related to this question. (1) What distinctions of user motivations are identified by previous research, and in what ways are the motivations of users amenable to quantitative analysis? (2) To what extent does tagging motivation vary across different social tagging systems? (3) How does variability in user motivation influence resulting tags and folksonomies? In this paper, we present measures to detect whether a tagger is primarily motivated by categorizing or describing resources, and apply these measures to datasets from seven different tagging systems. Our results show that (a) users’ motivation for tagging varies not only across, but also within tagging systems, and that (b) tag agreement among users who are motivated by categorizing resources is significantly lower than among users who are motivated by describing resources. Our findings are relevant for (1) the development of tag-based user interfaces, (2) the analysis of tag semantics and (3) the design of search algorithms for social tagging systems. PMID:23471473

  11. Tag retention, growth, and survival of red swamp crayfish marked with a visible implant tag

    USGS Publications Warehouse

    Isely, J.J.; Stockett, P.E.

    2001-01-01

    Eighty juvenile (means: 42.4 mm total length, 1.6 g) red swamp crayfish Procambarus clarkii were implanted with sequentially numbered visible implant tags and held in the laboratory. Tags were injected transversely into the musculature just beneath the exoskeleton of the third abdominal segment from the cephalothorax; tags were visible upon inspection. An additional 20 crayfish were left untagged and served as controls. After 150 d, tag retention was 80% and all tags were readable. No tagged crayfish died during the study, and no differences in total length or weight were detected between tagged and control crayfish. All individuals molted at least three times during the 150-d study, and some individuals molted up to six times, suggesting that most tags would be permanently retained. The readability in the field without specialized equipment makes the visible implant tag ideal for studies of crayfish ecology, management, and culture.

  12. Transferable green fluorescence-tagged pEI2 in Edwardsiella ictaluri

    USDA-ARS?s Scientific Manuscript database

    The pEI2 plasmid of Edwardsiella ictaluri isolate, I49, was tagged using a Tn10-GFP-kan cassette to create the green fluorescence-expressing derivative I49-gfp. The Tn10-GFP-kan insertion site was mapped by plasmid sequencing to 663 bp upstream of orf2 and appeared to be at a neutral site in the pla...

  13. STS-113 crew breakfast before second launch attempt

    NASA Technical Reports Server (NTRS)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. - On the second launch attempt, the STS-113 crew enjoys a snack before suiting up for launch. The launch was scrubbed on Nov. 22 because of poor weather in the Transoceanic Abort Landing sites. Seated left to right are Mission Specialists Michael Lopez-Alegria and John Herrington, Pilot Paul Lockhart and Commander James Wetherbee; Expedition 6 flight engineer Nikolai Budarin, Commander Ken Bowersox and flight engineer Donald Pettit. STS-113 is the 16th American assembly flight to the International Space Station. The launch will carry the Expedition 6 crew to the Station and return the Expedition 5 crew to Earth. The major objective of the mission is delivery of the Port 1 (P1) Integrated Truss Assembly, which will be attached to the port side of the S0 truss. Three spacewalks are planned to install and activate the truss and its associated equipment. Launch of Space Shuttle Endeavour on mission STS-113 is now scheduled for Nov. 23 at 7:50 p.m. EST.

  14. Multi-Threaded DNA Tag/Anti-Tag Library Generator for Multi-Core Platforms

    DTIC Science & Technology

    2009-05-01

    base pair)  Watson ‐ Crick  strand pairs that bind perfectly within pairs, but poorly across pairs. A variety  of  DNA  strand hybridization metrics...AFRL-RI-RS-TR-2009-131 Final Technical Report May 2009 MULTI-THREADED DNA TAG/ANTI-TAG LIBRARY GENERATOR FOR MULTI-CORE PLATFORMS...TYPE Final 3. DATES COVERED (From - To) Jun 08 – Feb 09 4. TITLE AND SUBTITLE MULTI-THREADED DNA TAG/ANTI-TAG LIBRARY GENERATOR FOR MULTI-CORE

  15. Fish tag recovery from Anaho Island nesting colony, Pyramid Lake, Nevada

    USGS Publications Warehouse

    Scoppettone, G. Gary; Fabes, Mark C.; Rissler, Peter H.; Withers, Donna

    2016-01-06

    In 2001, tags applied to the federally endangered species cui-ui (Chasmistes cujus) to study their population dynamics were discovered strewn throughout the American White Pelican (Pelecanus erythrorhynchos) nesting colony on Anaho Island, Pyramid Lake, Nevada. Cui-ui are endemic to Pyramid Lake, and Anaho Island harbors one of North America’s largest nesting colonies of American White Pelican. Cui-ui are consumed by pelicans during the fish’s spring migration into the Truckee River to reproduce. The predatory success of pelican has been validated by determining the odds of finding a tag from a predated cui-ui within the Anaho Island nesting colony. It is unknown how many cui-ui tags are eliminated by birds before arrival to the colony versus how many are brought to the colony but never recovered. The focus of this study was to improve the estimate of the chances of collecting a tag from a predated adult cui-ui in the pelican nesting colony by feeding dead tagged Lahontan cutthroat trout (Oncorhynchus clarkii henshawi) and common carp (Cyprinus carpio) to pelican and subsequently searching for these tags within the colony. We also randomly deployed 1,000 dispersal tags throughout the nesting colony, searching for these after one and two breeding seasons. After adding 1,027 fed fish to 547 previously fed fish, we estimated 5.3 percent of the tagged cui-ui taken by pelican were recovered during tag searches. A study of dispersal tags randomly deployed within the pelican nesting colony showed that 51.5 percent would be expected to be recovered after at least one breeding season after being deployed. Results of our studies indicate that more than 90 percent of tags from adult cui-ui are eliminated by birds outside the pelican nesting colony. Tags recovered from other species and the site at which they were tagged are also reported. Most notable were recovered Lahontan cutthroat trout tags, which were the highest in number, but their proximity to double

  16. Antenna for passive RFID tags

    NASA Astrophysics Data System (ADS)

    Schiopu, Paul; Manea, Adrian; Cristea, Ionica; Grosu, Neculai; Vladescu, Marian; Craciun, Anca-Ileana; Craciun, Alexandru

    2015-02-01

    Minuscule devices, called RFID tags are attached to objects and persons and emit information which positioned readers may capture wirelessly. Many methods of identification have been used, but that of most common is to use a unique serial number for identification of person or object. RFID tags can be characterized as either active or passive [1,2]. Traditional passive tags are typically in "sleep" state until awakened by the reader's emitted field. In passive tags, the reader's field acts to charge the capacitor that powers the badge and this can be a combination of antenna and barcodes obtained with SAW( Surface Acoustic Wave) devices [1,2,3] . The antenna in an RFID tag is a conductive element that permits the tag to exchange data with the reader. The paper contribution are targeted to antenna for passive RFID tags. The electromagnetic field generated by the reader is somehow oriented by the reader antenna and power is induced in the tag only if the orientation of the tag antenna is appropriate. A tag placed orthogonal to the reader yield field will not be read. This is the reason that guided manufacturers to build circular polarized antenna capable of propagating a field that is alternatively polarized on all planes passing on the diffusion axis. Passive RFID tags are operated at the UHF frequencies of 868MHz (Europe) and 915MHz (USA) and at the microwave frequencies of 2,45 GHz and 5,8 GHz . Because the tags are small dimensions, in paper, we present the possibility to use circular polarization microstrip antenna with fractal edge [2].

  17. Strep-Tagged Protein Purification.

    PubMed

    Maertens, Barbara; Spriestersbach, Anne; Kubicek, Jan; Schäfer, Frank

    2015-01-01

    The Strep-tag system can be used to purify recombinant proteins from any expression system. Here, protocols for lysis and affinity purification of Strep-tagged proteins from E. coli, baculovirus-infected insect cells, and transfected mammalian cells are given. Depending on the amount of Strep-tagged protein in the lysate, a protocol for batch binding and subsequent washing and eluting by gravity flow can be used. Agarose-based matrices with the coupled Strep-Tactin ligand are the resins of choice, with a binding capacity of up to 9 mg ml(-1). For purification of lower amounts of Strep-tagged proteins, the use of Strep-Tactin magnetic beads is suitable. In addition, Strep-tagged protein purification can also be automated using prepacked columns for FPLC or other liquid-handling chromatography instrumentation, but automated purification is not discussed in this protocol. The protocols described here can be regarded as an update of the Strep-Tag Protein Handbook (Qiagen, 2009). © 2015 Elsevier Inc. All rights reserved.

  18. Method for designing gas tag compositions

    DOEpatents

    Gross, Kenny C.

    1995-01-01

    For use in the manufacture of gas tags such as employed in a nuclear reactor gas tagging failure detection system, a method for designing gas tagging compositions utilizes an analytical approach wherein the final composition of a first canister of tag gas as measured by a mass spectrometer is designated as node #1. Lattice locations of tag nodes in multi-dimensional space are then used in calculating the compositions of a node #2 and each subsequent node so as to maximize the distance of each node from any combination of tag components which might be indistinguishable from another tag composition in a reactor fuel assembly. Alternatively, the measured compositions of tag gas numbers 1 and 2 may be used to fix the locations of nodes 1 and 2, with the locations of nodes 3-N then calculated for optimum tag gas composition. A single sphere defining the lattice locations of the tag nodes may be used to define approximately 20 tag nodes, while concentric spheres can extend the number of tag nodes to several hundred.

  19. Method for designing gas tag compositions

    DOEpatents

    Gross, K.C.

    1995-04-11

    For use in the manufacture of gas tags such as employed in a nuclear reactor gas tagging failure detection system, a method for designing gas tagging compositions utilizes an analytical approach wherein the final composition of a first canister of tag gas as measured by a mass spectrometer is designated as node No. 1. Lattice locations of tag nodes in multi-dimensional space are then used in calculating the compositions of a node No. 2 and each subsequent node so as to maximize the distance of each node from any combination of tag components which might be indistinguishable from another tag composition in a reactor fuel assembly. Alternatively, the measured compositions of tag gas numbers 1 and 2 may be used to fix the locations of nodes 1 and 2, with the locations of nodes 3-N then calculated for optimum tag gas composition. A single sphere defining the lattice locations of the tag nodes may be used to define approximately 20 tag nodes, while concentric spheres can extend the number of tag nodes to several hundred. 5 figures.

  20. WebTag: Web browsing into sensor tags over NFC.

    PubMed

    Echevarria, Juan Jose; Ruiz-de-Garibay, Jonathan; Legarda, Jon; Alvarez, Maite; Ayerbe, Ana; Vazquez, Juan Ignacio

    2012-01-01

    Information and Communication Technologies (ICTs) continue to overcome many of the challenges related to wireless sensor monitoring, such as for example the design of smarter embedded processors, the improvement of the network architectures, the development of efficient communication protocols or the maximization of the life cycle autonomy. This work tries to improve the communication link of the data transmission in wireless sensor monitoring. The upstream communication link is usually based on standard IP technologies, but the downstream side is always masked with the proprietary protocols used for the wireless link (like ZigBee, Bluetooth, RFID, etc.). This work presents a novel solution (WebTag) for a direct IP based access to a sensor tag over the Near Field Communication (NFC) technology for secure applications. WebTag allows a direct web access to the sensor tag by means of a standard web browser, it reads the sensor data, configures the sampling rate and implements IP based security policies. It is, definitely, a new step towards the evolution of the Internet of Things paradigm.

  1. WebTag: Web Browsing into Sensor Tags over NFC

    PubMed Central

    Echevarria, Juan Jose; Ruiz-de-Garibay, Jonathan; Legarda, Jon; Álvarez, Maite; Ayerbe, Ana; Vazquez, Juan Ignacio

    2012-01-01

    Information and Communication Technologies (ICTs) continue to overcome many of the challenges related to wireless sensor monitoring, such as for example the design of smarter embedded processors, the improvement of the network architectures, the development of efficient communication protocols or the maximization of the life cycle autonomy. This work tries to improve the communication link of the data transmission in wireless sensor monitoring. The upstream communication link is usually based on standard IP technologies, but the downstream side is always masked with the proprietary protocols used for the wireless link (like ZigBee, Bluetooth, RFID, etc.). This work presents a novel solution (WebTag) for a direct IP based access to a sensor tag over the Near Field Communication (NFC) technology for secure applications. WebTag allows a direct web access to the sensor tag by means of a standard web browser, it reads the sensor data, configures the sampling rate and implements IP based security policies. It is, definitely, a new step towards the evolution of the Internet of Things paradigm. PMID:23012511

  2. Auditory Magnetoencephalographic Frequency-Tagged Responses Mirror the Ongoing Segmentation Processes Underlying Statistical Learning.

    PubMed

    Farthouat, Juliane; Franco, Ana; Mary, Alison; Delpouve, Julie; Wens, Vincent; Op de Beeck, Marc; De Tiège, Xavier; Peigneux, Philippe

    2017-03-01

    Humans are highly sensitive to statistical regularities in their environment. This phenomenon, usually referred as statistical learning, is most often assessed using post-learning behavioural measures that are limited by a lack of sensibility and do not monitor the temporal dynamics of learning. In the present study, we used magnetoencephalographic frequency-tagged responses to investigate the neural sources and temporal development of the ongoing brain activity that supports the detection of regularities embedded in auditory streams. Participants passively listened to statistical streams in which tones were grouped as triplets, and to random streams in which tones were randomly presented. Results show that during exposure to statistical (vs. random) streams, tritone frequency-related responses reflecting the learning of regularities embedded in the stream increased in the left supplementary motor area and left posterior superior temporal sulcus (pSTS), whereas tone frequency-related responses decreased in the right angular gyrus and right pSTS. Tritone frequency-related responses rapidly developed to reach significance after 3 min of exposure. These results suggest that the incidental extraction of novel regularities is subtended by a gradual shift from rhythmic activity reflecting individual tone succession toward rhythmic activity synchronised with triplet presentation, and that these rhythmic processes are subtended by distinct neural sources.

  3. Tag loss and short-term mortality associated with passive integrated transponder tagging of juvenile Lost River suckers

    USGS Publications Warehouse

    Burdick, Summer M.

    2011-01-01

    Passive integrated transponder (PIT) tags are commonly used to mark small catostomids, but tag loss and the effect of tagging on mortality have not been assessed for juveniles of the endangered Lost River sucker Deltistes luxatus. I evaluated tag loss and short-term (34-d) mortality associated with the PIT tagging of juvenile Lost River suckers in the laboratory by using a completely randomized design and three treatment groups (PIT tagged, positive control, and control). An empty needle was inserted into each positive control fish, whereas control fish were handled but not tagged. Only one fish expelled its PIT tag. Mortality rate averaged 9.8 ± 3.4% (mean ± SD) for tagged fish; mortality was 0% for control and positive control fish. All tagging mortalities occurred in fish with standard lengths of 71 mm or less, and most of the mortalities occurred within 48 h of tagging. My results indicate that 12.45- × 2.02-mm PIT tags provide a viable method of marking juvenile Lost River suckers that are 72 mm or larger.

  4. High-affinity gold nanoparticle pin to label and localize histidine-tagged protein in macromolecular assemblies

    PubMed Central

    Anthony, Kelsey C.; You, Changjiang; Piehler, Jacob; Pomeranz Krummel, Daniel A.

    2014-01-01

    SUMMARY There is significant demand for experimental approaches to aid protein localization in electron microscopy micrographs and ultimately in three-dimensional reconstructions of macromolecular assemblies. We report preparation and use of a reagent consisting of tris-nitrilotriacetic acid (tris-NTA) conjugated with a monofunctional gold nanoparticle (AuNPtris-NTA) for site-specific, non-covalent labeling of protein termini fused to a histidine-tag (His-tag). Multivalent binding of tris-NTA to a His-tag via complexed Ni(II) ions results in subnanomolar affinity and a defined 1:1 stoichiometry. Precise localization of AuNPtris-NTA labeled proteins by electron microscopy is further ensured by the reagent’s short conformationally restricted linker. We have employed AuNPtris-NTA to localize His-tagged proteins in an oligomeric ATPase and in the bacterial 50S ribosomal subunit. AuNPtris-NTA can specifically bind to the target proteins in these assemblies and is clearly discernible. Our new labeling reagent should find broad application in non-covalent site-specific labeling of protein termini to pinpoint their location in macromolecular assemblies. PMID:24560806

  5. NASA Administrator Dan Goldin watches the STS-99 launch

    NASA Technical Reports Server (NTRS)

    2000-01-01

    KENNEDY SPACE CENTER, FLA. -- NASA Administrator Dan Goldin (right) joins other spectators at the Banana Creek viewing site in cheering the successful launch of Space Shuttle Endeavour on mission STS-99. The perfect liftoff occurred at 12:43:40 p.m. EST. Known as the Shuttle Radar Topography Mission (SRTM), STS-99 will chart a new course to produce unrivaled 3-D images of the Earth's surface. The result of the SRTM could be close to 1 trillion measurements of the Earth's topography. The mission is expected to last 11days, with Endeavour landing at KSC Tuesday, Feb. 22, at 4:36 p.m. EST. This is the 97th Shuttle flight and 14th for Shuttle Endeavour.

  6. Survival and tag loss in Moapa White River springfish implanted with passive integrated transponder tags

    USGS Publications Warehouse

    Dixon, Christopher J.; Mesa, Matthew G.

    2011-01-01

    We monitored survival and tag loss among Moapa White River springfish Crenichthys baileyi moapae that were surgically implanted with passive integrated transponder (PIT; 9 × 2 mm) tags. The fish used in the study ranged from 40 to 67 mm in total length and from 1.0 to 6.5 g in mass; the PIT tag: body weight ratios were 1.0–6.1%. Fish were held for 41 d in live cages within a small, warm desert stream. Survival did not differ between untagged control fish (94.5%) and tagged fish (95.6%). Survival did not appear to be influenced by fish size or PIT tag: body weight ratio, but the small number of fish that died precluded a detailed analysis. Tag retention was 100% among the 86 fish that survived over the 41 d. Our results suggest that surgically implanting 9-mm PIT tags into Moapa White River springfish as small as 40 mm is an effective method for marking them because it has minimal impacts on survival and tag retention is high. More work is needed on the effects of PIT tagging on growth and other performance metrics of springfish and other small desert fishes.

  7. Vehicle Tracking System using Nanotechnology Satellites and Tags

    NASA Technical Reports Server (NTRS)

    Lorenzini, Dino A.; Tubis, Chris

    1995-01-01

    This paper describes a joint project to design, develop, and deploy a satellite based tracking system incorporating micro-nanotechnology components. The system consists of a constellation of 'nanosats', a satellite command station and data collection sites, and a large number of low-cost electronic 'tags'. Both government and commercial applications are envisioned for the satellite based tracking system. The projected low price for the tracking service is made possible by the lightweight nanosats and inexpensive electronic tags which use high production volume single chip transceivers and microprocessor devices. The nanosat consists of a five inch aluminum cube with body mounted solar panels (GaAs solar cells) on all six faces. A UHF turnstile antenna and a simple, spring release mechanism complete the external configuration of the spacecraft.

  8. Social Tagging of Mission Data

    NASA Technical Reports Server (NTRS)

    Norris, Jeffrey S.; Wallick, Michael N.; Joswig, Joseph C.; Powell, Mark W.; Torres, Recaredo J.; Mittman, David S.; Abramyan, Lucy; Crockett, Thomas M.; Shams, Khawaja S.; Fox, Jason M.; hide

    2010-01-01

    Mars missions will generate a large amount of data in various forms, such as daily plans, images, and scientific information. Often, there is a semantic linkage between images that cannot be captured automatically. Software is needed that will provide a method for creating arbitrary tags for this mission data so that items with a similar tag can be related to each other. The tags should be visible and searchable for all users. A new routine was written to offer a new and more flexible search option over previous applications. This software allows users of the MSLICE program to apply any number of arbitrary tags to a piece of mission data through a MSLICE search interface. The application of tags creates relationships between data that did not previously exist. These tags can be easily removed and changed, and contain enough flexibility to be specifically configured for any mission. This gives users the ability to quickly recall or draw attention to particular pieces of mission data, for example: Give a semantic and meaningful description to mission data; for example, tag all images with a rock in them with the tag "rock." Rapidly recall specific and useful pieces of data; for example, tag a plan as"driving template." Call specific data to a user s attention; for example, tag a plan as "for:User." This software is part of the MSLICE release, which was written in Java. It will run on any current Windows, Macintosh, or Linux system.

  9. STS-113 Mission Specialist John Herrington suits up for second launch attempt

    NASA Technical Reports Server (NTRS)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. -- - STS-113 Mission Specialist John Herrington smiles as he finishes suiting up for a second launch attempt on mission STS-113. The launch on Nov. 22 was scrubbed due to poor weather conditions at the Transoceanic Abort Landing sites. Herrington will be making his first Shuttle flight. The launch will carry the Expedition 6 crew to the Station and return the Expedition 5 crew to Earth. The major objective of the mission is delivery of the Port 1 (P1) Integrated Truss Assembly, which will be attached to the port side of the S0 truss. Three spacewalks are planned to install and activate the truss and its associated equipment. Launch of Space Shuttle Endeavour on mission STS-113 is now scheduled for Nov. 23 at 7:50 p.m. EST.

  10. Mission support plan STS-2

    NASA Technical Reports Server (NTRS)

    Ibanez, F.

    1981-01-01

    The plan defines the anticipated GSTDN/DOD station support and configuration requirements for a nominal flight with an orbital inclination of 38.4 degrees and a circular orbit of 120 nautical miles for the first 5 orbits and 137 nautical miles thereafter. A complete set of preliminary site configuration messages (SCM) define nominal station AOS/LOS times and configurations for S-Band and UHF support. This document is intended for use as a planning tool, providing the necessary guidelines and data base for SCM generation in support of STS-2.

  11. Genetically encoded fluorescent tags

    PubMed Central

    Thorn, Kurt

    2017-01-01

    Genetically encoded fluorescent tags are protein sequences that can be fused to a protein of interest to render it fluorescent. These tags have revolutionized cell biology by allowing nearly any protein to be imaged by light microscopy at submicrometer spatial resolution and subsecond time resolution in a live cell or organism. They can also be used to measure protein abundance in thousands to millions of cells using flow cytometry. Here I provide an introduction to the different genetic tags available, including both intrinsically fluorescent proteins and proteins that derive their fluorescence from binding of either endogenous or exogenous fluorophores. I discuss their optical and biological properties and guidelines for choosing appropriate tags for an experiment. Tools for tagging nucleic acid sequences and reporter molecules that detect the presence of different biomolecules are also briefly discussed. PMID:28360214

  12. Landing of Space Shuttle Atlantis / STS-125 Mission

    NASA Image and Video Library

    2009-05-24

    STS125-S-066 (24 May 2009) --- The STS-125 crew pose for a photo near Space Shuttle Atlantis on Runway 22 at Edwards Air Force Base in California following their landing which ended the STS-125 mission to repair and upgrade NASA?s Hubble Space Telescope. From the left are astronauts Mike Massimino, mission specialist; Gregory C. Johnson, pilot; Scott Altman, commander; Megan McArthur, John Grunsfeld, Andrew Feustel and Michael Good, all mission specialists. The main landing gear touched down at 8:39:05 a.m. (PDT) on May 24, 2009. Nose gear touchdown was at 8:39:15 a.m. Wheel-stop was at 8:40:15 a.m., bringing the mission?s elapsed time to 12 days, 21 hours, 37 minutes, 9 seconds. Landing opportunities on May 22, May 23 and May 24 were waved off due to weather concerns at NASA?s Kennedy Space Center in Florida, the shuttle?s primary landing site. Through five spacewalks, Hubble was refurbished and upgraded with state-of-the-art science instruments that will expand Hubble's capabilities and extend its operational lifespan through at least 2014.

  13. Rhabdomyomatous mesenchymal hamartoma presenting as a skin tag in the sternoclavicular area.

    PubMed

    Solis-Coria, Araceli; Vargas-González, Roberto; Sotelo-Avila, Cirilo

    2007-01-01

    Rhabdomyomatous mesenchymal hamartoma (RMH) is a rare congenital lesion of the dermis and subdermis. It has been described predominantly in newborns, with 30 cases reported in the English literature. Typically, it appears as a skin tag, papule, nodule or a mass involving the face or sternal notch. A 28-day-old girl presented with a 1.4 x 0.8 cm soft skin tag in the right sternoclavicular area. Physical examination revealed no congenital anomalies. A shaved biopsy showed that the core of the lesion contained striated muscle fibers mixed with hair follicles and sebaceous and eccrine glands. Thin epidermis lined the outside of the tag. We report a patient with a RMH in a site not previously reported and discuss the differential diagnosis.

  14. STS-128 patch Pathd

    NASA Image and Video Library

    2009-04-14

    STS128-S-001 (April 2009) --- The STS-128 patch symbolizes the 17A mission and represents the hardware, people and partner nations that contribute to the flight. The Space shuttle Discovery is shown in the orbit configuration with the Multi-Purpose Logistics Module (MPLM) Leonardo in the payload bay. Earth and the International Space Station wrap around the Astronaut Office symbol reminding us of the continuous human presence in space. The names of the STS-128 crew members border the patch in an unfurled manner. Included in the names is the expedition crew member who will launch on STS-128 and remain on board ISS, replacing another Expedition crew member who will return home with STS-128. The banner also completes the Astronaut Office symbol and contains the U.S. and Swedish flags representing the countries of the STS-128 crew. The NASA insignia design for space shuttle flights is reserved for use by the astronauts and for other official use as the NASA Administrator may authorize. Public availability has been approved only in the forms of illustrations by the various news media. When and if there is any change in this policy, which is not anticipated, the change will be publicly announced. Photo credit: NASA

  15. Review on SAW RFID tags.

    PubMed

    Plessky, Victor P; Reindl, Leonhard M

    2010-03-01

    SAW tags were invented more than 30 years ago, but only today are the conditions united for mass application of this technology. The devices in the 2.4-GHz ISM band can be routinely produced with optical lithography, high-resolution radar systems can be built up using highly sophisticated, but low-cost RF-chips, and the Internet is available for global access to the tag databases. The "Internet of Things," or I-o-T, will demand trillions of cheap tags and sensors. The SAW tags can overcome semiconductor-based analogs in many aspects: they can be read at a distance of a few meters with readers radiating power levels 2 to 3 orders lower, they are cheap, and they can operate in robust environments. Passive SAW tags are easily combined with sensors. Even the "anti-collision" problem (i.e., the simultaneous reading of many nearby tags) has adequate solutions for many practical applications. In this paper, we discuss the state-of-the-art in the development of SAW tags. The design approaches will be reviewed and optimal tag designs, as well as encoding methods, will be demonstrated. We discuss ways to reduce the size and cost of these devices. A few practical examples of tags using a time-position coding with 10(6) different codes will be demonstrated. Phase-coded devices can additionally increase the number of codes at the expense of a reduction of reading distance. We also discuss new and exciting perspectives of using ultra wide band (UWB) technology for SAW-tag systems. The wide frequency band available for this standard provides a great opportunity for SAW tags to be radically reduced in size to about 1 x 1 mm(2) while keeping a practically infinite number of possible different codes. Finally, the reader technology will be discussed, as well as detailed comparison made between SAW tags and IC-based semiconductor device.

  16. Tag retention, growth, and survival of red swamp crayfish Procambarus clarkii marked with coded wire tags

    USGS Publications Warehouse

    Isely, J.J.; Eversole, A.G.

    1998-01-01

    Juvenile red swamp crayfish (or crawfish), Procambarus clarkii (20-41 mm in total length) were collected from a crayfish culture pond by dipnetting and tagged with sequentially numbered, standard length, binary-coded wire tags. Four replicates of 50 crayfish were impaled perpendicular to the long axis of the abdomen with a fixed needle. Tags were injected transversely into the ventral surface of the first or second abdominal segment and were imbedded in the musculature just beneath the abdominal sternum. Tags were visible upon inspection. Additionally, two replicates of 50 crayfish were not tagged and were used as controls. Growth, survival, and tag retention were evaluated after 7 d in individual containers, after 100 d in aquaria, and after 200 d in field cages. Tag retention during each sample period was 100%, and average mortality of tagged crayfish within 7 d of tagging was 1%. Mortality during the remainder of the study was high (75-91%) but was similar between treatment and control samples. Most of the deaths were probably due to cannibalism. Average total length increased threefold during the course of the study, and crayfish reached maturity. Because crayfish were mature by the end of the study, we concluded that the coded wire tag was retained through the life history of the crayfish.

  17. OARE STS-94 (MSL-1R)

    NASA Technical Reports Server (NTRS)

    Rice, James E.

    1998-01-01

    The report is organized into sections representing the phases of work performed in analyzing the STS-94 (MSL-IR) results. STS-94 (MSL1R) is a reflight of the STS-83 (MSL-1) mission which was terminated early because of a fuel cell problem. Section I briefly outlines the OARE system features, coordinates, and measurement parameters. Section 2 describes the results from STS-94. The mission description, data calibration, and representative data obtained on STS-94 are presented. Also, the anomalous performance of OARE on STS-94 is discussed. Finally, Section 3 presents a discussion of accuracy achieved and achievable with OARE. Appendix A discuss the calibration and data processing methodology in detail.

  18. STS_135_Landing

    NASA Image and Video Library

    2011-07-21

    NASA astronaut Chris Ferguson, STS-135 commander, examines the thermal tiles of the orbiter after the space shuttle Atlantis landed at the Kennedy Space Center in Florida completing STS-135, the final mission of the NASA shuttle program, on Thursday, July 21, 2011. ( NASA Photo / Houston Chronicle, Smiley N. Pool )

  19. Improving temperature monitoring in the vaccine cold chain at the periphery: an intervention study using a 30-day electronic refrigerator temperature logger (Fridge-tag).

    PubMed

    Kartoğlu, Umit; Nelaj, Erida; Maire, Denis

    2010-05-28

    This intervention study was conducted in Albania to establish the superiority of the Fridge-tag (30-day electronic refrigerator temperature logger) against thermometers. Intervention sites used Fridge-tag and a modified temperature control record sheet, while control sites continued with their routine operation with thermometers. All refrigerators in both groups were equipped with downloadable electronic data loggers to record temperatures for reference. Focus group sessions were conducted with involved staff to discuss temperature monitoring, Fridge-tag use and its user-friendliness. Significant discrepancies were observed between thermometer readings and the electronic data loggers in control sites, while all alarms from Fridge-tag were confirmed in the intervention group. Thermometers are not sufficient to monitor temperatures in refrigerators since they miss the great majority of low and high alarms. Fridge-tag has proven to be an effective tool in providing health workers with the information they need to take the necessary actions when there are refrigerator temperature variations. (c) 2010 Elsevier Ltd. All rights reserved.

  20. Directional Radio-Frequency Identification Tag Reader

    NASA Technical Reports Server (NTRS)

    Medelius, Pedro J.; Taylor, John D.; Henderson, John J.

    2004-01-01

    A directional radio-frequency identification (RFID) tag reader has been designed to facilitate finding a specific object among many objects in a crowded room. The device could be an adjunct to an electronic inventory system that tracks RFID-tagged objects as they move through reader-equipped doorways. Whereas commercial RFID-tag readers do not measure directions to tagged objects, the device is equipped with a phased-array antenna and a received signal-strength indicator (RSSI) circuit for measuring direction. At the beginning of operation, it is set to address only the RFID tag of interest. It then continuously transmits a signal to interrogate that tag while varying the radiation pattern of the antenna. It identifies the direction to the tag as the radiation pattern direction of peak strength of the signal returned by the tag. An approximate distance to the tag is calculated from the peak signal strength. The direction and distance can be displayed on a screen. A prototype containing a Yagi antenna was found to be capable of detecting a 915.5-MHz tag at a distance of approximately equal to 15 ft (approximately equal to 4.6 m).

  1. The ATLAS TAGS database distribution and management - Operational challenges of a multi-terabyte distributed database

    NASA Astrophysics Data System (ADS)

    Viegas, F.; Malon, D.; Cranshaw, J.; Dimitrov, G.; Nowak, M.; Nairz, A.; Goossens, L.; Gallas, E.; Gamboa, C.; Wong, A.; Vinek, E.

    2010-04-01

    The TAG files store summary event quantities that allow a quick selection of interesting events. This data will be produced at a nominal rate of 200 Hz, and is uploaded into a relational database for access from websites and other tools. The estimated database volume is 6TB per year, making it the largest application running on the ATLAS relational databases, at CERN and at other voluntary sites. The sheer volume and high rate of production makes this application a challenge to data and resource management, in many aspects. This paper will focus on the operational challenges of this system. These include: uploading the data from files to the CERN's and remote sites' databases; distributing the TAG metadata that is essential to guide the user through event selection; controlling resource usage of the database, from the user query load to the strategy of cleaning and archiving of old TAG data.

  2. Evaluation of Intercontinental Transport of Ozone Using Full-tagged, Tagged-N and Sensitivity Methods

    NASA Astrophysics Data System (ADS)

    Guo, Y.; Liu, J.; Mauzerall, D. L.; Emmons, L. K.; Horowitz, L. W.; Fan, S.; Li, X.; Tao, S.

    2014-12-01

    Long-range transport of ozone is of great concern, yet the source-receptor relationships derived previously depend strongly on the source attribution techniques used. Here we describe a new tagged ozone mechanism (full-tagged), the design of which seeks to take into account the combined effects of emissions of ozone precursors, CO, NOx and VOCs, from a particular source, while keeping the current state of chemical equilibrium unchanged. We label emissions from the target source (A) and background (B). When two species from A and B sources react with each other, half of the resulting products are labeled A, and half B. Thus the impact of a given source on downwind regions is recorded through tagged chemistry. We then incorporate this mechanism into the Model for Ozone and Related chemical Tracers (MOZART-4) to examine the impact of anthropogenic emissions within North America, Europe, East Asia and South Asia on ground-level ozone downwind of source regions during 1999-2000. We compare our results with two previously used methods -- the sensitivity and tagged-N approaches. The ozone attributed to a given source by the full-tagged method is more widely distributed spatially, but has weaker seasonal variability than that estimated by the other methods. On a seasonal basis, for most source/receptor pairs, the full-tagged method estimates the largest amount of tagged ozone, followed by the sensitivity and tagged-N methods. In terms of trans-Pacific influence of ozone pollution, the full-tagged method estimates the strongest impact of East Asian (EA) emissions on the western U.S. (WUS) in MAM and JJA (~3 ppbv), which is substantially different in magnitude and seasonality from tagged-N and sensitivity studies. This difference results from the full-tagged method accounting for the maintenance of peroxy radicals (e.g., CH3O2, CH3CO3, and HO2), in addition to NOy, as effective reservoirs of EA source impact across the Pacific, allowing for a significant contribution to

  3. A new approach to tag design in dolphin telemetry: Computer simulations to minimise deleterious effects

    NASA Astrophysics Data System (ADS)

    Pavlov, V. V.; Wilson, R. P.; Lucke, K.

    2007-02-01

    Remote-sensors and transmitters are powerful devices for studying cetaceans at sea. However, despite substantial progress in microelectronics and miniaturisation of systems, dolphin tags are imperfectly designed; additional drag from tags increases swim costs, compromises swimming capacity and manoeuvrability, and leads to extra loads on the animal's tissue. We propose a new approach to tag design, elaborating basic principles and incorporating design stages to minimise device effects by using computer-aided design. Initially, the operational conditions of the device are defined by quantifying the shape, hydrodynamics and range of the natural deformation of the dolphin body at the tag attachment site (such as close to the dorsal fin). Then, parametric models of both of the dorsal fin and a tag are created using the derived data. The link between parameters of the fin and a tag model allows redesign of tag models according to expected changes of fin geometry (difference in fin shape related with species, sex, and age peculiarities, simulation of the bend of the fin during manoeuvres). A final virtual modelling stage uses iterative improvement of a tag model in a computer fluid dynamics (CFD) environment to enhance tag performance. This new method is considered as a suitable tool of tag design before creation of the physical model of a tag and testing with conventional wind/water tunnel technique. Ultimately, tag materials are selected to conform to the conditions identified by the modelling process and thus help create a physical model of a tag, which should minimise its impact on the animal carrier and thus increase the reliability and quality of the data obtained.

  4. An active site–tail interaction in the structure of hexahistidine-tagged Thermoplasma acidophilum citrate synthase

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Murphy, Jesse R.; Donini, Stefano; Kappock, T. Joseph, E-mail: kappock@purdue.edu

    2015-09-23

    Citrate synthase from the thermophilic euryarchaeon T. acidophilum fused to a hexahistidine tag was purified and biochemically characterized. The structure of the unliganded enzyme at 2.2 Å resolution contains tail–active site contacts in half of the active sites. Citrate synthase (CS) plays a central metabolic role in aerobes and many other organisms. The CS reaction comprises two half-reactions: a Claisen aldol condensation of acetyl-CoA (AcCoA) and oxaloacetate (OAA) that forms citryl-CoA (CitCoA), and CitCoA hydrolysis. Protein conformational changes that ‘close’ the active site play an important role in the assembly of a catalytically competent condensation active site. CS from themore » thermoacidophile Thermoplasma acidophilum (TpCS) possesses an endogenous Trp fluorophore that can be used to monitor the condensation reaction. The 2.2 Å resolution crystal structure of TpCS fused to a C-terminal hexahistidine tag (TpCSH6) reported here is an ‘open’ structure that, when compared with several liganded TpCS structures, helps to define a complete path for active-site closure. One active site in each dimer binds a neighboring His tag, the first nonsubstrate ligand known to occupy both the AcCoA and OAA binding sites. Solution data collectively suggest that this fortuitous interaction is stabilized by the crystalline lattice. As a polar but almost neutral ligand, the active site–tail interaction provides a new starting point for the design of bisubstrate-analog inhibitors of CS.« less

  5. Artist concept drawings of STS-47 and STS-50 Spacelab (SL) experiments

    NASA Technical Reports Server (NTRS)

    1992-01-01

    Artist concept drawings of STS-47 Endeavour, Orbiter Vehicle 105, Spacelab Japan (SL-J) and STS-50 Columbia, Orbiter Vehicle (OV) 102, United States Microgravity Laboratory 1 (USML-1) experiments include glovebox (41097) and lower body negative pressure (LBNP) device (41098).

  6. STS-99 launch is cheered by NASA Administrator Dan Goldin

    NASA Technical Reports Server (NTRS)

    2000-01-01

    NASA Administrator Dan Goldin (right) joins other spectators at the Banana Creek viewing site in cheering the successful launch of Space Shuttle Endeavour on mission STS-99. The perfect liftoff occurred at 12:43:40 p.m. EST. Known as the Shuttle Radar Topography Mission (SRTM), STS-99 will chart a new course to produce unrivaled 3-D images of the Earth's surface. The result of the SRTM could be close to 1 trillion measurements of the Earth's topography. The mission is expected to last 11days, with Endeavour landing at KSC Tuesday, Feb. 22, at 4:36 p.m. EST. This is the 97th Shuttle flight and 14th for Shuttle Endeavour.

  7. Remote Calibration Procedure and Results for the Ctbto AS109 STS-2HG at Ybh

    NASA Astrophysics Data System (ADS)

    Uhrhammer, R. A.; Taira, T.; Hellweg, M.

    2013-12-01

    Berkeley Digital Seismic Station (BDSN) YBH, located in Yreka, CA, USA, is certified as Auxiliary Seismic Station 109 (AS109) by the Preparatory Commission for the Comprehensive Nuclear-Test-Ban Treaty organization (CTBTO). YBH, sited in an abandoned hard rock mining drift, houses a Streckeisen STS-2HG triaxial broadband seismometer (the AS109 sensor) and a co-sited three-component set of Streckeisen STS-1 broadband seismometers and a Kinemetrics Episensor strong motion accelerometer (the BDSN sensors). CTBTO requested that we preform a remote calibration test of the STS-2HG (20,000 V/(m/s) nominal sensitivity) to verify its response and sensitivity. The remote calibration test was done successfully on June 17, 2013 and we report here on the procedure and results of the calibration. The calibration of the STS-2HG (s/n 30235) was accomplished using two Random Telegraph (RT) stimuli which were applied to the triaxial U,V,W component calibration coils through an appropriate series resistance to limit the drive current. The first was a four hour RT at 1.25 Hz (to determine the low-frequency response) and the second was a one hour RT at 25 Hz (to determine the high-frequency response). The RT stimulus signals were generated by the Kinemetrics Q330 data logger and both the stimuli and the response were recorded simultaneously with synchronous sampling at 100 sps. The RT calibrations were invoked remotely from Berkeley. The response to the 1.25 Hz RT stimulus was used to determine the seismometer natural period, fraction of critical damping and sensitivity of the STS-2HG sensors and the response to the 25 Hz RT stimulus was used to determine their corresponding high-frequency response. The accuracy of the sensitivity as determined by the response to the RT stimuli is limited by the accuracy of the calibration coil motor constant (2 g/A) provided on the factory calibration sheet. As a check on the accuracy of the sensitivity determined from the response to the RT stimuli

  8. Spectators in the stands watch launch of STS-95 and Space Shuttle Discovery.

    NASA Technical Reports Server (NTRS)

    1998-01-01

    These stands are filled with spectators watching and photographing the launch of STS-95. The viewing sites and roadways at Kennedy Space Center bulge with people and vehicles wanting to see Space Shuttle Discovery lift off. Extra attention has been drawn to the mission due to the addition to the crew of John H. Glenn Jr., a senator from Ohio. STS-95 is Glenn's second flight into space after 36 years; he was one of the original Project Mercury astronauts and flew his first mission in February 1962. The STS-95 mission includes research payloads such as the Spartan solar-observing deployable spacecraft, the Hubble Space Telescope Orbital Systems Test Platform, the International Extreme Ultraviolet Hitchhiker, as well as a SPACEHAB single module with experiments on space flight and the aging process.

  9. Earth Observation from space taken during Mission STS-111 UF-2

    NASA Image and Video Library

    2002-06-05

    STS111-719-061 (5-19 June 2002) --- This photo showing the Manicouagan Reservoir in Quebec, Canada, was photographed by the STS-111 crewmembers aboard the Space Shuttle Endeavour. Manicouagan Reservoir marks the site of an impact crater, 60 miles (100 km) wide, which, according to scientists, was formed 212 million years ago when a meteorite crashed into this area. Scientists say that over millions of years the many advancing and retreating glaciers and other erosional processes have worn down the crater. The Gateway to Astronaut Photography of Earth (link to http://eol.jsc.nasa.gov/sseop/) provides searchable access to other photographs of Earth taken by astronauts.

  10. STS_135_CEIT

    NASA Image and Video Library

    2011-04-07

    JSC2011-E-040358 (7 April 2011) --- NASA astronaut Doug Hurley, STS-135 pilot, exits the hatch of the space shuttle Atlantis during the STS-135 Crew Equipment Interface Test (CEIT) in the Orbiter Processing Facility at NASA?s Kennedy Space Center, Florida on April 7, 2011. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

  11. STS-34 earth observations

    NASA Image and Video Library

    1989-10-20

    STS034-44-023 (20 Oct. 1989) --- The Southern Lights or Aurora Australis were photographed by the STS-34 crewmembers aboard the Earth-orbiting Space Shuttle Atlantis. From the Shuttle astronauts can photograph expanses of auroras, an advantage over scientists on Earth who can only get small sections at a time in a frame of photography. The space position allows for large-scale changes. This scene was one of 26 shown to the press by the five STS-34 crewmembers at their post-flight press conference.

  12. Buddy Tag CONOPS and Requirements.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Brotz, Jay Kristoffer; Deland, Sharon M.

    2015-12-01

    This document defines the concept of operations (CONOPS) and the requirements for the Buddy Tag, which is conceived and designed in collaboration between Sandia National Laboratories and Princeton University under the Department of State Key VerificationAssets Fund. The CONOPS describe how the tags are used to support verification of treaty limitations and is only defined to the extent necessary to support a tag design. The requirements define the necessary functions and desired non-functional features of the Buddy Tag at a high level

  13. Osmium Tag for Posttranscriptionally Modified RNA.

    PubMed

    Debnath, Turja Kanti; Okamoto, Akimitsu

    2018-05-25

    Nucleotide modifications of cellular RNA are highly abundant and diverse, but their origin and functions have not yet been investigated. 5-Methylcytidine (m5C) and 5-methyluridine (m5U) are highly abundant posttranscriptionally modified nucleotides observed in various natural RNAs. Such nucleotides have been labeled through a chemical approach as both undergo oxidation at the C5-C6 double bond, leading to the formation of osmium-bipyridine complexes, which are identified by mass spectrometry. This osmium tag made it possible to distinguished m5C and m5U from their isomers 2'-O-methylcytidine and 2'-O-methyluridine, respectively. Queuosine and 2-methylthio-N6-isopentenyladenosine in tRNA were also tagged through this complex formation--this is the first time that this has ever been achieved. Osmylation has emerged as a structure-selective reaction and largely governed by the environment of the target site (the steric and higher order structure), therefore it could be helpful for studying the structure and dynamics of RNA-protein interactions. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. Optimal use of tandem biotin and V5 tags in ChIP assays

    PubMed Central

    Kolodziej, Katarzyna E; Pourfarzad, Farzin; de Boer, Ernie; Krpic, Sanja; Grosveld, Frank; Strouboulis, John

    2009-01-01

    Background Chromatin immunoprecipitation (ChIP) assays coupled to genome arrays (Chip-on-chip) or massive parallel sequencing (ChIP-seq) lead to the genome wide identification of binding sites of chromatin associated proteins. However, the highly variable quality of antibodies and the availability of epitopes in crosslinked chromatin can compromise genomic ChIP outcomes. Epitope tags have often been used as more reliable alternatives. In addition, we have employed protein in vivo biotinylation tagging as a very high affinity alternative to antibodies. In this paper we describe the optimization of biotinylation tagging for ChIP and its coupling to a known epitope tag in providing a reliable and efficient alternative to antibodies. Results Using the biotin tagged erythroid transcription factor GATA-1 as example, we describe several optimization steps for the application of the high affinity biotin streptavidin system in ChIP. We find that the omission of SDS during sonication, the use of fish skin gelatin as blocking agent and choice of streptavidin beads can lead to significantly improved ChIP enrichments and lower background compared to antibodies. We also show that the V5 epitope tag performs equally well under the conditions worked out for streptavidin ChIP and that it may suffer less from the effects of formaldehyde crosslinking. Conclusion The combined use of the very high affinity biotin tag with the less sensitive to crosslinking V5 tag provides for a flexible ChIP platform with potential implications in ChIP sequencing outcomes. PMID:19196479

  15. Novel Concrete Temperature Monitoring Method Based on an Embedded Passive RFID Sensor Tag.

    PubMed

    Liu, Yongsheng; Deng, Fangming; He, Yigang; Li, Bing; Liang, Zhen; Zhou, Shuangxi

    2017-06-22

    This paper firstly introduces the importance of temperature control in concrete measurement, then a passive radio frequency identification (RFID) sensor tag embedded for concrete temperature monitoring is presented. In order to reduce the influences of concrete electromagnetic parameters during the drying process, a T-type antenna is proposed to measure the concrete temperature at the required depth. The proposed RFID sensor tag is based on the EPC generation-2 ultra-high frequency (UHF) communication protocol and operates in passive mode. The temperature sensor can convert the sensor signals to corresponding digital signals without an external reference clock due to the adoption of phase-locked loop (PLL)-based architecture. Laboratory experimentation and on-site testing demonstrate that our sensor tag embedded in concrete can provide reliable communication performance in passive mode. The maximum communicating distance between reader and tag is 7 m at the operating frequency of 915 MHz and the tested results show high consistency with the results tested by a thermocouple.

  16. Novel Concrete Temperature Monitoring Method Based on an Embedded Passive RFID Sensor Tag

    PubMed Central

    Liu, Yongsheng; Deng, Fangming; He, Yigang; Li, Bing; Liang, Zhen; Zhou, Shuangxi

    2017-01-01

    This paper firstly introduces the importance of temperature control in concrete measurement, then a passive radio frequency identification (RFID) sensor tag embedded for concrete temperature monitoring is presented. In order to reduce the influences of concrete electromagnetic parameters during the drying process, a T-type antenna is proposed to measure the concrete temperature at the required depth. The proposed RFID sensor tag is based on the EPC generation-2 ultra-high frequency (UHF) communication protocol and operates in passive mode. The temperature sensor can convert the sensor signals to corresponding digital signals without an external reference clock due to the adoption of phase-locked loop (PLL)-based architecture. Laboratory experimentation and on-site testing demonstrate that our sensor tag embedded in concrete can provide reliable communication performance in passive mode. The maximum communicating distance between reader and tag is 7 m at the operating frequency of 915 MHz and the tested results show high consistency with the results tested by a thermocouple. PMID:28640188

  17. STS-134 Flight Controllers on Console - Launch.

    NASA Image and Video Library

    2011-05-16

    JSC2011-E-044228 (16 May 2011) --- Flight director Tony Ceccacci is pictured at his console in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center during launch countdown activities a few hundred miles away in Florida, site of space shuttle Endeavour's STS-134 launch. Liftoff was at 8:56 a.m. (EDT) on May 16, 2011, from Launch Pad 39A at NASA's Kennedy Space Center. Photo credit: NASA

  18. STS-29 crew activities

    NASA Image and Video Library

    2000-04-19

    STS029-04-029 (13-18 March 1989) --- Astronaut Michael L. Coats appears to like the status of the STS-29 flight as he offers a big smile from the commander's station on the flight deck. He takes a momentary break from updating the crew activity plan (CAP) to pose for the photo. This photographic frame was among NASA's third STS-29 photo release. Monday, March 20, 1989. Crew members were astronauts Michael L. Coats, John E. Blaha, James F. Buchli, Robert C. Springer and James P. Bagian. Photo credit: NASA

  19. SparkClouds: visualizing trends in tag clouds.

    PubMed

    Lee, Bongshin; Riche, Nathalie Henry; Karlson, Amy K; Carpendale, Sheelash

    2010-01-01

    Tag clouds have proliferated over the web over the last decade. They provide a visual summary of a collection of texts by visually depicting the tag frequency by font size. In use, tag clouds can evolve as the associated data source changes over time. Interesting discussions around tag clouds often include a series of tag clouds and consider how they evolve over time. However, since tag clouds do not explicitly represent trends or support comparisons, the cognitive demands placed on the person for perceiving trends in multiple tag clouds are high. In this paper, we introduce SparkClouds, which integrate sparklines into a tag cloud to convey trends between multiple tag clouds. We present results from a controlled study that compares SparkClouds with two traditional trend visualizations—multiple line graphs and stacked bar charts—as well as Parallel Tag Clouds. Results show that SparkClouds ability to show trends compares favourably to the alternative visualizations.

  20. STS-135_VMS

    NASA Image and Video Library

    2011-03-02

    JSC2011-E-040204 (2 March 2011) --- NASA astronaut Chris Ferguson, STS-135 commander, prepares for departure from Moffett Field in a T-38 trainer home to Houston after the crew of STS-135 trained in the Vertical Motion Simulator (VMS) at NASA's Ames Research Center in Mountain View, Calif. on March 2, 2011, Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

  1. STS_135_CEIT

    NASA Image and Video Library

    2011-04-08

    JSC2011-E-040366 (8 April 2011) --- Close-up photo of tools taken during the STS-135 crew members' inspection of the equipment they will use in space. The inspection was part of the STS-135 Crew Equipment Interface Test (CEIT) conducted April 8, 2011 at NASA?s Kennedy Space Center in Florida. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

  2. A model for estimating passive integrated transponder (PIT) tag antenna efficiencies for interval-specific emigration rates

    USGS Publications Warehouse

    Horton, G.E.; Dubreuil, T.L.; Letcher, B.H.

    2007-01-01

    Our goal was to understand movement and its interaction with survival for populations of stream salmonids at long-term study sites in the northeastern United States by employing passive integrated transponder (PIT) tags and associated technology. Although our PIT tag antenna arrays spanned the stream channel (at most flows) and were continuously operated, we are aware that aspects of fish behavior, environmental characteristics, and electronic limitations influenced our ability to detect 100% of the emigration from our stream site. Therefore, we required antenna efficiency estimates to adjust observed emigration rates. We obtained such estimates by testing a full-scale physical model of our PIT tag antenna array in a laboratory setting. From the physical model, we developed a statistical model that we used to predict efficiency in the field. The factors most important for predicting efficiency were external radio frequency signal and tag type. For most sampling intervals, there was concordance between the predicted and observed efficiencies, which allowed us to estimate the true emigration rate for our field populations of tagged salmonids. One caveat is that the model's utility may depend on its ability to characterize external radio frequency signals accurately. Another important consideration is the trade-off between the volume of data necessary to model efficiency accurately and the difficulty of storing and manipulating large amounts of data.

  3. STS-124 and Expedition 17 crew portrait

    NASA Image and Video Library

    2008-06-09

    S124-E-007905 (9 June 2008) --- The STS-124 and Expedition 17 crewmembers pose for a group portrait following a joint news conference from the newly installed Kibo Japanese Pressurized Module of the International Space Station while Space Shuttle Discovery is docked with the station. From the left (front row) are NASA astronauts Karen Nyberg, Garrett Reisman, both STS-124 mission specialists; Mark Kelly, STS-124 commander; Russian Federal Space Agency cosmonaut Sergei Volkov, Expedition 17 commander; and NASA astronaut Mike Fossum, STS-124 mission specialist. From the left (back row) are NASA astronaut Ron Garan, STS-124 mission specialist; Russian Federal Space Agency cosmonaut Oleg Kononenko, Expedition 17 flight engineer; NASA astronauts Ken Ham, STS-124 pilot; Greg Chamitoff, Expedition 17 flight engineer; and Japan Aerospace Exploration Agency astronaut Akihiko Hoshide, STS-124 mission specialist. Reisman, who joined the station's crew in March, is being replaced by Chamitoff, who arrived at the station with the STS-124 crew.

  4. The novel use of pop-off satellite tags (PSATs) to investigate the migratory behaviour of European sea bass Dicentrarchus labrax.

    PubMed

    O'Neill, R; Ó Maoiléidigh, N; McGinnity, P; Bond, N; Culloty, S

    2018-05-01

    A total of 12 adult European sea bass Dicentrarchus labrax were tagged with pop-off satellite archival tags (PSAT) in Irish coastal waters and in offshore waters in the north-east Celtic Sea between 2015 and 2016. Archived data were successfully recovered from five of the 12 tags deployed, three from fish released in inshore Irish waters and two from fish released offshore in the eastern Celtic Sea. All three fish tagged in inshore waters were found to undertake migrations into the open ocean coinciding with the spawning period. These fish also exhibited fidelity to inshore sites post-migration, returning to the same general location (within c. 73 km, which is roughly the predicted mean accuracy of the method) of their original release site. Although the number of tracks obtained here was limited, some degree of aggregation between inshore and offshore tagged fish in the eastern Celtic Sea was noted during the expected spawning period suggesting PSATs can provide new information on specific spawning locations of European sea bass. © 2018 The Fisheries Society of the British Isles.

  5. Establishing nursery estuary otolith geochemical tags for Sea Bass (Dicentrarchus labrax): Is temporal stability estuary dependent?

    NASA Astrophysics Data System (ADS)

    Ryan, Diarmuid; Wögerbauer, Ciara; Roche, William

    2016-12-01

    The ability to determine connectivity between juveniles in nursery estuaries and adult populations is an important tool for fisheries management. Otoliths of juvenile fish contain geochemical tags, which reflect the variation in estuarine elemental chemistry, and allow discrimination of their natal and/or nursery estuaries. These tags can be used to investigate connectivity patterns between juveniles and adults. However, inter-annual variability of geochemical tags may limit the accuracy of nursery origin determinations. Otolith elemental composition was used to assign a single cohort of 0-group sea bass Dicentrarchus labrax to their nursery estuary thus establishing an initial baseline for stocks in waters around Ireland. Using a standard LDFA model, high classification accuracies to nursery sites (80-88%) were obtained. Temporal stability of otolith geochemical tags was also investigated to assess if annual sampling is required for connectivity studies. Geochemical tag stability was found to be strongly estuary dependent.

  6. Survival, growth, and tag retention in age-0 Chinook Salmon implanted with 8-, 9-, and 12-mm PIT tags

    USGS Publications Warehouse

    Tiffan, Kenneth F.; Perry, Russell W.; Connor, William P.; Mullins, Frank L.; Rabe, Craig; Nelson, Doug D

    2015-01-01

    The ability to represent a population of migratory juvenile fish with PIT tags becomes difficult when the minimum tagging size is larger than the average size at which fish begin to move downstream. Tags that are smaller (e.g., 8 and 9 mm) than the commonly used 12-mm PIT tags are currently available, but their effects on survival, growth, and tag retention in small salmonid juveniles have received little study. We evaluated growth, survival, and tag retention in age-0 Chinook Salmon Oncorhynchus tshawytscha of three size-groups: 40–49-mm fish were implanted with 8- and 9-mm tags, and 50– 59-mm and 60–69-mm fish were implanted with 8-, 9-, and 12-mm tags. Survival 28 d after tagging ranged from 97.8% to 100% across all trials, providing no strong evidence for a fish-size-related tagging effect or a tag size effect. No biologically significant effects of tagging on growth in FL (mm/d) or weight (g/d) were observed. Although FL growth in tagged fish was significantly reduced for the 40–49-mm and 50–59-mm groups over the first 7 d, growth rates were not different thereafter, and all fish were similar in size by the end of the trials (day 28). Tag retention across all tests ranged from 93% to 99%. We acknowledge that actual implantation of 8- or 9-mm tags into small fish in the field will pose additional challenges (e.g., capture and handling stress) beyond those observed in our laboratory. However, we conclude that experimental use of the smaller tags for small fish in the field is supported by our findings.

  7. Comparing the hierarchy of author given tags and repository given tags in a large document archive

    NASA Astrophysics Data System (ADS)

    Tibély, Gergely; Pollner, Péter; Palla, Gergely

    2016-10-01

    Folksonomies - large databases arising from collaborative tagging of items by independent users - are becoming an increasingly important way of categorizing information. In these systems users can tag items with free words, resulting in a tripartite item-tag-user network. Although there are no prescribed relations between tags, the way users think about the different categories presumably has some built in hierarchy, in which more special concepts are descendants of some more general categories. Several applications would benefit from the knowledge of this hierarchy. Here we apply a recent method to check the differences and similarities of hierarchies resulting from tags given by independent individuals and from tags given by a centrally managed repository system. The results from our method showed substantial differences between the lower part of the hierarchies, and in contrast, a relatively high similarity at the top of the hierarchies.

  8. STS_135_STA

    NASA Image and Video Library

    2011-05-31

    JSC2011-E-059480 (31 May 2011) --- NASA astronaut Sandy Magnus, STS-135 mission specialist, is seen on May 31 in the rear station of a T-38 which had been piloted by astronaut Doug Hurley, STS-135 pilot, and is now sitting just off the runway following arrival at NASA?s Kennedy Space Center in Florida. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

  9. Improved Tandem Affinity Purification Tag and Methods for Isolation of Proteins and Protein Complexes from Schizosaccharomyces pombe.

    PubMed

    Zilio, Nicola; Boddy, Michael N

    2017-03-01

    The tandem affinity purification (TAP) method uses an epitope that contains two different affinity purification tags separated by a site-specific protease site to isolate a protein rapidly and easily. Proteins purified via the TAP tag are eluted under mild conditions, allowing them to be used for structural and biochemical analyses. The original TAP tag contains a calmodulin-binding peptide and the IgG-binding domain from protein A separated by a tobacco etch virus (TEV) protease cleavage site. After capturing the Protein A epitope on an IgG resin, bound proteins are released by incubation with the TEV protease and then isolated on a calmodulin matrix in the presence of calcium; elution from this resin is achieved by chelating calcium with EGTA. However, because the robustness of the calmodulin-binding step in this procedure is highly variable, we replaced the calmodulin-binding peptide with three copies of the FLAG epitope, (3× FLAG)-TEV-Protein A, which can be isolated using an anti-FLAG resin. Elution from this matrix is achieved in the presence of an excess of a 3× FLAG peptide. In addition to allowing proteins to be released under mild conditions, elution by the 3× FLAG peptide adds an extra layer of specificity to the TAP procedure, because it liberates only FLAG-tagged proteins. © 2017 Cold Spring Harbor Laboratory Press.

  10. STS-119 Launch Skyline

    NASA Image and Video Library

    2009-03-15

    STS119-S-025 (15 March 2009) --- The setting sun paints the clouds over NASA's Kennedy Space Center in Florida before the launch of Space Shuttle Discovery on the STS-119 mission. Liftoff is scheduled for 7:43 p.m. (EDT) on March 15, 2009.

  11. STS-133 and Expedition 26 Crewmembers

    NASA Image and Video Library

    2011-02-26

    ISS026-E-030338 (26 Feb. 2011) --- STS-133 and Expedition 26 crew members are pictured shortly after space shuttle Discovery and the International Space Station docked in space and the hatches were opened. Pictured are NASA astronauts Scott Kelly (left foreground), Expedition 26 commander; Steve Bowen (left) and Michael Barratt (right foreground), both STS-133 mission specialists. Visible in the background are NASA astronauts Eric Boe (left), STS-133 pilot; and Steve Lindsey (mostly obscured at right), STS-133 commander.

  12. STS-73 (USML-2) Final Report

    NASA Technical Reports Server (NTRS)

    Rice, James E.

    1996-01-01

    The report is organized into sections representing the phases of work performed in analyzing the STS-73 (USML-2) results. Section 1 briefly outlines the Orbital Acceleration Research Experiment (OARE), system features, coordinates, and measurement parameters. Section 2 describes the results from STS-73. The mission description, data calibration, and representative data obtained on STS-73 are presented. Also, the anomalous performance of OARE on STS-73 is discussed. Finally, Section 3 presents a discussion of accuracy achieved and achievable with OARE.

  13. STS-32 Earth observation of the western Coral Sea and the Great Barrier Reef

    NASA Image and Video Library

    1990-01-20

    STS032-520-014 (9-20 Jan. 1990) --- STS-32 astronauts took this 70mm scene showing phytoplankton oralgal bloom in the northwest Coral Sea. The Western Coral Sea and the Great Barrier Reef waters offshore Queensland, Australia are the sites of some of the larger concentrations or "blooms" of phytoplankton and algae in the open ocean. In the instance illustrated here, the leading edge of a probable concentration of algae or phytoplankton is seen as a light irregular line and sheen between the offshore Great Barrier Reef and the Queensland coast. Previous phytoplankton concentrations in this area have been reported by ships at sea as having formed floating mats as thick as two meters. This picture was used by the STS-32 astronauts at their Jan. 30, 1990 post-flight press conference.

  14. Notes on SAW Tag Interrogation Techniques

    NASA Technical Reports Server (NTRS)

    Barton, Richard J.

    2010-01-01

    We consider the problem of interrogating a single SAW RFID tag with a known ID and known range in the presence of multiple interfering tags under the following assumptions: (1) The RF propagation environment is well approximated as a simple delay channel with geometric power-decay constant alpha >/= 2. (2) The interfering tag IDs are unknown but well approximated as independent, identically distributed random samples from a probability distribution of tag ID waveforms with known second-order properties, and the tag of interest is drawn independently from the same distribution. (3) The ranges of the interfering tags are unknown but well approximated as independent, identically distributed realizations of a random variable rho with a known probability distribution f(sub rho) , and the tag ranges are independent of the tag ID waveforms. In particular, we model the tag waveforms as random impulse responses from a wide-sense-stationary, uncorrelated-scattering (WSSUS) fading channel with known bandwidth and scattering function. A brief discussion of the properties of such channels and the notation used to describe them in this document is given in the Appendix. Under these assumptions, we derive the expression for the output signal-to-noise ratio (SNR) for an arbitrary combination of transmitted interrogation signal and linear receiver filter. Based on this expression, we derive the optimal interrogator configuration (i.e., transmitted signal/receiver filter combination) in the two extreme noise/interference regimes, i.e., noise-limited and interference-limited, under the additional assumption that the coherence bandwidth of the tags is much smaller than the total tag bandwidth. Finally, we evaluate the performance of both optimal interrogators over a broad range of operating scenarios using both numerical simulation based on the assumed model and Monte Carlo simulation based on a small sample of measured tag waveforms. The performance evaluation results not only

  15. High Level Expression and Purification of Recombinant Proteins from Escherichia coli with AK-TAG

    PubMed Central

    Luo, Dan; Wen, Caixia; Zhao, Rongchuan; Liu, Xinyu; Liu, Xinxin; Cui, Jingjing; Liang, Joshua G.; Liang, Peng

    2016-01-01

    Adenylate kinase (AK) from Escherichia coli was used as both solubility and affinity tag for recombinant protein production. When fused to the N-terminus of a target protein, an AK fusion protein could be expressed in soluble form and purified to near homogeneity in a single step from Blue-Sepherose via affinity elution with micromolar concentration of P1, P5- di (adenosine—5’) pentaphosphate (Ap5A), a transition-state substrate analog of AK. Unlike any other affinity tags, the level of a recombinant protein expression in soluble form and its yield of recovery during each purification step could be readily assessed by AK enzyme activity in near real time. Coupled to a His-Tag installed at the N-terminus and a thrombin cleavage site at the C terminus of AK, the streamlined method, here we dubbed AK-TAG, could also allow convenient expression and retrieval of a cleaved recombinant protein in high yield and purity via dual affinity purification steps. Thus AK-TAG is a new addition to the arsenal of existing affinity tags for recombinant protein expression and purification, and is particularly useful where soluble expression and high degree of purification are at stake. PMID:27214237

  16. STS-130 Launch-on-Need (LON) Assessment

    NASA Technical Reports Server (NTRS)

    Jezierski, Eduardo; Margasahayam, Ravi; McCarter, Dallas; Stampfel, Andrew

    2011-01-01

    A viewgraph presentation covering an STS-130 Launch on Need assessment is shown. The contents include: 1) LON Status GREEN II STS-132 is processing as the LON for STS-131; 2) TSM Bonnet Closure Timing; 3) LC-39A High Pressure Gas Storage Facility (HPGF) Net Damage; and 4) STS-130 Ice Detection Camera FOD concern.

  17. sts111-s-008

    NASA Image and Video Library

    2002-06-05

    STS111-S-008 (5 June 2002) --- The Space Shuttle Endeavour leaves the launch pad, headed into space for mission STS-111 to the International Space Station (ISS). Liftoff occurred at 5:22:49 p.m. (EDT), June 5, 2002. The STS-111 crew includes astronauts Kenneth D. Cockrell, commander; Paul S. Lockhart, pilot, and Franklin R. Chang-Diaz and Philippe Perrin, mission specialists. Also onboard were the Expedition Five crew members including cosmonaut Valery G. Korzun, commander, along with astronaut Peggy A. Whitson and cosmonaut Sergei Y. Treschev, flight engineers. Perrin represents CNES, the French space agency, and Korzun and Treschev are with the Russian Aviation and Space Agency (Rosaviakosmos). This mission marks the 14th Shuttle flight to the International Space Station and the third Shuttle mission this year. Mission STS-111 is the 18th flight of Endeavour and the 110th flight overall in NASA's Space Shuttle program.

  18. STS_135_NBL

    NASA Image and Video Library

    2011-03-10

    JSC2011-E-040220 (10 March 2011) --- NASA astronaut Rex Walheim (left), STS-135 mission specialist, and astronaut Mike Fossum are aided by divers as they work in a mock-up of the space shuttle's payload bay as the crew of STS-135 trains for a spacewalk in the Neutral Buoyancy Laboratory near NASA?s Johnson Space Center on March 10, 2011. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

  19. STS_135_NBL

    NASA Image and Video Library

    2011-03-10

    JSC2011-E-040218 (10 March 2011) --- NASA astronaut Rex Walheim, STS-135 mission specialist, is aided by divers as he works with astronaut Mike Fossum in a mock-up of the space shuttle's payload bay as the crew of STS-135 trains for a spacewalk in the Neutral Buoyancy Laboratory near NASA?s Johnson Space Center on March 10, 2011. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

  20. 49 CFR 234.239 - Tagging of wires and interference of wires or tags with signal apparatus.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... with signal apparatus. 234.239 Section 234.239 Transportation Other Regulations Relating to... Tagging of wires and interference of wires or tags with signal apparatus. Each wire shall be tagged or... of the apparatus. This requirement applies to each wire at each terminal in all housings including...

  1. 49 CFR 234.239 - Tagging of wires and interference of wires or tags with signal apparatus.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... with signal apparatus. 234.239 Section 234.239 Transportation Other Regulations Relating to... Tagging of wires and interference of wires or tags with signal apparatus. Each wire shall be tagged or... of the apparatus. This requirement applies to each wire at each terminal in all housings including...

  2. STS123-S-001

    NASA Image and Video Library

    2007-09-30

    STS123-S-001 (Oct. 2007) --- STS-123 continues assembly of the International Space Station (ISS). The primary mission objectives include rotating an expedition crew member and installing both the first component of the Japanese Experimental Module (the Experimental Logistics Module - Pressurized Section (ELM-PS)) and the Canadian Special Purpose Dexterous Manipulator (SPDM). In addition, STS-123 will deliver various spare ISS components and leave behind the sensor boom used for inspecting the shuttle's thermal protection system. A follow-on mission to ISS will utilize and then return home with this sensor boom. A total of four spacewalks are planned to accomplish these tasks. The mission will also require the use of both the shuttle and ISS robotic arms. STS-123 will utilize the Station-Shuttle Power Transfer System to extend the docked portion of the mission to eleven days, with a total planned duration of 15 days. The crew patch depicts the space shuttle in orbit with the crew names trailing behind. STS-123's major additions to ISS (the ELM-PS installation with the shuttle robotic arm and the fully constructed SPDM) are both illustrated. The ISS is shown in the configuration that the STS-123 crew will encounter when they arrive. The NASA insignia design for space shuttle flights is reserved for use by the astronauts and for other official use as the NASA Administrator may authorize. Public availability has been approved only in the forms of illustrations by the various news media. When and if there is any change in this policy, which is not anticipated, the change will be publicly announced. Photo credit: NASA

  3. Soldier Data Tag Study Effort.

    DTIC Science & Technology

    1985-06-10

    interested in protecting it. The tag itself is difficult--though not impossible--to counterfeit . Also, it (’• iii 71 -, potentially improves the data...attacks during the design, manufacture, and distribution processes, counterfeiting , unauthorized access/alteration of tag data, and use of the tag to...45 3.3.2 Hijacking of SOT System Shipments, or Large- Scale Counterfeit of SOT Systems ....................... 46 3.3.3 Unauthorized Alteration

  4. STS payload data collection and accommodations analysis study. Volume 3: Accommodations analysis

    NASA Technical Reports Server (NTRS)

    1978-01-01

    Payload requirements were compared to launch site accommodations and flight accommodations for a number of Spacelab payloads. Experiment computer operating system accommodations were also considered. A summary of accommodations in terms of resources available for payload discretionary use and recommendations for Spacelab/STS accommodation improvements are presented.

  5. OARE STS-87 (USMP-4)

    NASA Technical Reports Server (NTRS)

    Rice, James E.

    1998-01-01

    The report is organized into sections representing the phases of work performed in analyzing the STS-87 (USMP-4) results. Section 1 briefly outlines the OARE system features, coordinates, and measurement parameters. Section 2 describes the results from STS-87. The mission description, data calibration, and representative data obtained on STS-87 are presented. Finally, Section 3 presents a discussion of accuracy achieved and achievable with OARE. Appendix A discusses the calibration and data processing methodology in detail.

  6. STS126-S-002

    NASA Image and Video Library

    2008-03-01

    STS126-S-002 (5 March 2008) --- Attired in training versions of their shuttle launch and entry suits, these seven astronauts take a break from training to pose for the STS-126 crew portrait. Astronaut Christopher J. Ferguson, commander, is at center; and astronaut Eric A. Boe, pilot, is third from the right. Remaining crewmembers, pictured from left to right, are astronauts Sandra H. Magnus, Stephen G. Bowen, Donald R. Pettit, Robert S. (Shane) Kimbrough and Heidemarie M. Stefanyshyn-Piper, all mission specialists. Magnus is scheduled to join Expedition 18 as flight engineer after launching to the International Space Station on mission STS-126.

  7. Uncertainty of exploitation estimates made from tag returns

    USGS Publications Warehouse

    Miranda, L.E.; Brock, R.E.; Dorr, B.S.

    2002-01-01

    Over 6,000 crappies Pomoxis spp. were tagged in five water bodies to estimate exploitation rates by anglers. Exploitation rates were computed as the percentage of tags returned after adjustment for three sources of uncertainty: postrelease mortality due to the tagging process, tag loss, and the reporting rate of tagged fish. Confidence intervals around exploitation rates were estimated by resampling from the probability distributions of tagging mortality, tag loss, and reporting rate. Estimates of exploitation rates ranged from 17% to 54% among the five study systems. Uncertainty around estimates of tagging mortality, tag loss, and reporting resulted in 90% confidence intervals around the median exploitation rate as narrow as 15 percentage points and as broad as 46 percentage points. The greatest source of estimation error was uncertainty about tag reporting. Because the large investments required by tagging and reward operations produce imprecise estimates of the exploitation rate, it may be worth considering other approaches to estimating it or simply circumventing the exploitation question altogether.

  8. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses

    PubMed Central

    Baker, Oliver; Gupta, Ashish; Obst, Mandy; Zhang, Youming; Anastassiadis, Konstantinos; Fu, Jun; Stewart, A. Francis

    2016-01-01

    A fluent method for gene targeting to establish protein tagged and ligand inducible conditional loss-of-function alleles is described. We couple new recombineering applications for one-step cloning of gRNA oligonucleotides and rapid generation of short-arm (~1 kb) targeting constructs with the power of Cas9-assisted targeting to establish protein tagged alleles in embryonic stem cells at high efficiency. RAC (Recombineering And Cas9)-tagging with Venus, BirM, APEX2 and the auxin degron is facilitated by a recombineering-ready plasmid series that permits the reuse of gene-specific reagents to insert different tags. Here we focus on protein tagging with the auxin degron because it is a ligand-regulated loss-of-function strategy that is rapid and reversible. Furthermore it includes the additional challenge of biallelic targeting. Despite high frequencies of monoallelic RAC-targeting, we found that simultaneous biallelic targeting benefits from long-arm (>4 kb) targeting constructs. Consequently an updated recombineering pipeline for fluent generation of long arm targeting constructs is also presented. PMID:27216209

  9. STS-74 clears tower (with view of RSS)

    NASA Technical Reports Server (NTRS)

    1995-01-01

    The STS-74 astronauts depart the Operations and Checkout Building, headed for the launch pad and a rendezvous in space. Leading the way are Commander Kenneth D. Cameron (front right) and Pilot James D. Halsell Jr. (front left). Behind them are the three mission specialists assigned to STS-74 (front to back): Chris A. Hadfield, representing the Canadian Space Agency; Jerry L. Ross, and William S. 'Bill' McArthur Jr. Awaiting them at Launch Pad 39A is the Space Shuttle Atlantis, scheduled for a second liftoff attempt lift off during a seven-minute launch window opening at about 7:30 a.m. EST, Nov. 12. During its approximately eight-day flight, Atlantis will dock with the Russian Space Station Mir and a permanent docking extension will be attached to the station, and transfer of materials to and from the mated spacecraft will be completed. A first launch attempt Nov. 11 was scrubbed due to unfavorable weather conditions at the contingency Transoceanic Abort Landing (TAL) sites.

  10. STS118-S-073

    NASA Image and Video Library

    2007-08-21

    STS118-S-073 (21 Aug. 2007) --- NASA Administrator Michael Griffin (center in white shirt) and other NASA managers give thumbs up signals to the crew of the Space Shuttle Endeavour shortly after its touchdown August 21, 2007, at the Kennedy Space Center, Florida, concluding STS-118. Photo Credit: NASA/Bill Ingalls

  11. Earth observations taken during STS-102 flight

    NASA Image and Video Library

    2001-03-18

    STS102-303-017 (8-21 March 2001)--- The STS-102 crew members used a 35mm camera on the flight deck of the Space Shuttle Discovery to record this image of the Aswan High Dam. The structure was completed in 1970 and is one of the largest earthen embankment dams in the world. It is 364 feet (111 meters) tall, 12,565 feet (3,830 meters) long and nearly 3,281 feet (1,000 meters) wide. When it was built the new reservoir required relocation of nearly 100,000 residents and some archaeological sites. Although the reservoir has benefited Egypt by providing power and controlling floods, according to NASA scientists, it has also had detrimental effects on the Nile system. Before the dam, an estimated 110 million tons of silt was deposited by the annual flood of the Nile, enriching agricultural lands and maintaining the land of the Nile delta. Now this sediment is trapped behind the dam, requiring artificial fertilization of agricultural lands and leading to erosion and saltwater intrusion where the Nile river meets the Mediterranean Sea.

  12. Use of fluorescent tagging for assessment of environmental cleaning and disinfection in a veterinary hospital.

    PubMed

    Weese, J S; Lowe, T; Walker, M

    2012-09-01

    Environmental cleaning was assessed at a small animal veterinary referral hospital and associated primary healthcare facility. A convenience sample of surfaces was contaminated with fluorescent dye, and then cleaning was assessed 24 hours later by UV light visualisation. Five hundred sixty-three sites were assessed; however, 70 sites were unable to be evaluated 24 hours later because equipment had been removed or because rooms were occupied at the time of re-evaluation. Overall, dye was removed from 212/493 (43%) of sites. Site-specific rates ranged from 14% (computer keyboards and mice, 9/66 site cleaned) to 81% (examination tables, 44/54 sites cleaned). There was a significant difference in the prevalence of successful cleaning by general location (P < 0.0001) and surface type (P < 0.0001). Environmental tagging was an easy and low-cost tool to assess cleaning practices. Results prompted further infection control investigations to explain selected deficiencies, leading to identification of inadequacies in protocols and practices. Environmental tagging may be a useful infection control tool for establishing baseline cleaning rates, identifying deficiencies in protocols, evaluating the effects of interventions and education of personnel.

  13. Z-path SAW RFID tag.

    PubMed

    Härmä, Sanna; Plessky, Victor P; Hartmann, Clinton S; Steichen, William

    2008-01-01

    Surface acoustic wave (SAW) radio-frequency identification (RFID) tags are soon expected to be produced in very high volumes. The size and cost of a SAW RFID tag will be key parameters for many applications. Therefore, it is of primary importance to reduce the chip size. In this work, we describe the design principles of a 2.4-GHz SAW RFID tag that is significantly smaller than earlier reported tags. We also present simulated and experimental results. The coded signal should arrive at the reader with a certain delay (typically about 1 micros), i.e., after the reception of environmental echoes. If the tag uses a bidirectional interdigital transducer (IDT), space for the initial delay is needed on both sides of the IDT. In this work, we replace the bidirectional IDT by a unidirectional one. This halves the space required by the initial delay because all the code reflectors must now be placed on the same side of the IDT. We reduce tag size even further by using a Z-path geometry in which the same space in x-direction is used for both the initial delay and the code reflectors. Chip length is thus determined only by the space required by the code reflectors.

  14. STS-122 flight controllers in WFCR during launch

    NASA Image and Video Library

    2008-02-07

    JSC2008-E-010344 (7 Feb. 2008) --- Flight directors Norm Knight (left), Bryan Lunney and Richard Jones monitor data at their consoles in the space shuttle flight control room of Johnson Space Center's Mission Control Center (MCC) during launch countdown activities a few hundred miles away in Florida, site of Space Shuttle Atlantis' scheduled STS-122 launch. Liftoff occurred at 2:45 p.m. (EST) on Feb. 7, 2008 from launch pad 39A at Kennedy Space Center.

  15. Earth observations taken by the STS-59 crew

    NASA Image and Video Library

    1994-04-14

    STS059-306-034 (9-20 April 1994) --- The radiating transportation pattern of this 20th-Century urban plan is nicely displayed in this high-resolution photograph. Light-toned spots in the desert mark oil well sites; along the south edge of the scene, some dark residue remains from the fires set during the recent Gulf War. Blown sand has nearly hidden the soot and thick oil that were deposited on the surface. Nikon photograph, 300mm lens.

  16. [Radiobiological effects on plants and animals within Semipalatinsk Test Site (Kazakhstan)].

    PubMed

    Mozolin, E M; Geras'kin, S A; Minkenova, K S

    2008-01-01

    The Semipalatinsk Test Site (STS) was the main place of nuclear devices tests in the former Soviet Union. From 1949 to 1989 about 460 nuclear explosions have been carried out at STS. Radioactive contamination of STS territory has the extremely non-uniform character. The main dose-forming radionuclides are 137Cs, 90Sr, 152Eu, as well as 154Eu, 60CO, 239,240Pu and 241Am. The greatest specific activity of 137Cs and 239,240Pu in ground are n x 10(3) kBk/kg, 152Eu - 96 kBk/kg, 154Eu - 10.4 kBk/kg, 60Co - 20.5 kBk/kg, 241Am - 15 kBk/kg. However, up to now, within STS sites exists where gamma-dose rate comes to 60 microGy/h, that is enough for induction reliable biological effects in animals and plants. Inhabiting territory of STS plants and animals are characterized by increased level of mutagenesis, changes of morpho-anatomic indices and parameters of peripheral blood, by the increase of asymmetry bilateral indices, change of composition and structure of communities.

  17. STS_135_Return

    NASA Image and Video Library

    2011-07-22

    JSC2011-E-068761 (22 July 2011) --- A small portion of a large Ellington Field crowd is seen on July 22, 2011 through a door bearing a STS-135 sticker on its window. A short while later the crew of the space shuttle Atlantis' mission used this door for its entrance during a welcome home ceremony. STS-135 is the final mission of the NASA Space Shuttle Program. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

  18. STS_135_Landing

    NASA Image and Video Library

    2011-07-21

    JSC2011-E-067995 (21 July 2011) --- NASA astronaut Chris Ferguson, STS-135 commander, makes a public statement as, from left, NASA Administrator Charles Bolden, along with NASA astronauts Rex Walheim, Sandy Magnus and Doug Hurley look on after the space shuttle Atlantis landed on July 21 at the Kennedy Space Center in Florida. The landing completed STS-135, the final mission of the NASA Space Shuttle Program. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

  19. Scalable Faceted Ranking in Tagging Systems

    NASA Astrophysics Data System (ADS)

    Orlicki, José I.; Alvarez-Hamelin, J. Ignacio; Fierens, Pablo I.

    Nowadays, web collaborative tagging systems which allow users to upload, comment on and recommend contents, are growing. Such systems can be represented as graphs where nodes correspond to users and tagged-links to recommendations. In this paper we analyze the problem of computing a ranking of users with respect to a facet described as a set of tags. A straightforward solution is to compute a PageRank-like algorithm on a facet-related graph, but it is not feasible for online computation. We propose an alternative: (i) a ranking for each tag is computed offline on the basis of tag-related subgraphs; (ii) a faceted order is generated online by merging rankings corresponding to all the tags in the facet. Based on the graph analysis of YouTube and Flickr, we show that step (i) is scalable. We also present efficient algorithms for step (ii), which are evaluated by comparing their results with two gold standards.

  20. 40 CFR 35.4070 - How can my group spend TAG money?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... grant; and/or (3) Provide one-time health and safety training for your technical advisor to gain site... 40 Protection of Environment 1 2012-07-01 2012-07-01 false How can my group spend TAG money? 35.4070 Section 35.4070 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL...

  1. Earth observations of the Bahamas and Florida taken during STS-99

    NASA Image and Video Library

    2000-03-09

    STS099-729-086 (11-22 February 2000) ---In this 70mm frame photographed by one of the STS-99 crew members, the city of Miami encroaches the eastern edge of the Everglades in Florida. The Everglades is an International Biosphere Reserve World Heritage Site. This subtropical wilderness encompasses a relatively flat (does not exceed 2.4 meters above sea level) saw-grass marsh region of 10,000 square kilometers (4,000 square miles). The only source of water in the Everglades is from rainfall. The flow of water is detectable in this image, slowly moving from Lake Okeechobee to Florida Bay; the light blue, shallow area (less than 3 meters) between the mainland and the Keys; and the southwestern Florida coast.

  2. Tags, wireless communication systems, tag communication methods, and wireless communications methods

    DOEpatents

    Scott,; Jeff W. , Pratt; Richard, M [Richland, WA

    2006-09-12

    Tags, wireless communication systems, tag communication methods, and wireless communications methods are described. In one aspect, a tag includes a plurality of antennas configured to receive a plurality of first wireless communication signals comprising data from a reader, a plurality of rectifying circuits coupled with. respective individual ones of the antennas and configured to provide rectified signals corresponding to the first wireless communication signals, wherein the rectified signals are combined to produce a composite signal, an adaptive reference circuit configured to vary a reference signal responsive to the composite signal, a comparator coupled with the adaptive reference circuit and the rectifying circuits and configured to compare the composite signal with respect to the reference signal and to output the data responsive to the comparison, and processing circuitry configured to receive the data from the comparator and to process the data.

  3. Retinal Photography during STS-34

    NASA Image and Video Library

    2009-07-01

    STS034-10-015 (18-23 Oct. 1989) --- Astronaut Franklin R. Chang-Diaz performs an eye examination on astronaut Ellen S. Baker, both STS-34 mission specialists, on the middeck of the Earth-orbiting space shuttle Atlantis.

  4. Mission Safety Evaluation Report for STS-32, Postflight Edition

    NASA Technical Reports Server (NTRS)

    Hill, William C.; Finkel, Seymour I.

    1990-01-01

    The topics covered include: (1) an STS-32 mission summary; (2) safety risk factors/issues; (3) resolved STS-32 safety risk factors; (4) STS-32 inflight anomalies; (5) STS-28 inflight anomalies; and (6) STS-32 inflight anomalies. Background information and a list of acronyms are also presented.

  5. STS-71 preflight crew portrait

    NASA Image and Video Library

    1995-03-05

    STS071-S-002 (5 March 1995) --- Crew members for the STS-71 mission and the related Mir missions assemble for a crew portrait at the Johnson Space Center (JSC). In front are, left to right, Vladimir N. Dezhurov, Robert L. Gibson and Anatoliy Y. Solovyev, mission commanders for Mir-18, STS-71 and Mir-19, respectively. On the back row are, left to right, Norman E. Thagard, Gennadiy M. Strekalov, Gregory J. Harbaugh, Ellen S. Baker, Charles J. Precourt, Bonnie J. Dunbar and Nikolai M. Budarin. In a precedent-setting flight, Thagard later this month will be launched as a guest researcher along with Dezhurov, commander, and Strekalov, flight engineer, to Russia's Mir Space Station for a three month mission, designated as Mir 18. Then in late spring, as the assignment of STS-71, the Space Shuttle Atlantis will rendezvous with the Russian Mir Space Station to pick up the Mir 18 crew and transfer cosmonauts Solovyev and Budarin to the station for the Mir 19 mission. The STS-71 crew members are Gibson, commander; Precourt, pilot; and Harbaugh, Baker and Dunbar mission specialists.

  6. Global Warming and Ozone Layer Depletion: STS Issues for Social Studies Classrooms.

    ERIC Educational Resources Information Center

    Rye, James A.; Strong, Donna D.; Rubba, Peter A.

    2001-01-01

    Explores the inclusion of science-technology-society (STS) education in social studies. Provides background information on global warming and the depletion of the ozone layer. Focuses on reasons for teaching global climate change in the social studies classroom and includes teaching suggestions. Offers a list of Web sites about global climate…

  7. STS_135_Launch

    NASA Image and Video Library

    2011-07-08

    JSC2011-E-067589 (8 July 2011) --- The space shuttle Atlantis launches for the STS-135 mission to the International Space Station in the final mission of the Space Shuttle Program at NASA?s Kennedy Space Center in Florida. Liftoff was at 11:29 a.m. (EDT) on July 8, 2011. Onboard are NASA astronauts Chris Ferguson, STS-135 commander; Doug Hurley, pilot; Sandy Magnus and Rex Walheim, both mission specialists. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

  8. STS_135_Launch

    NASA Image and Video Library

    2011-07-09

    JSC2011-E-067644 (8 July 2011) --- The space shuttle Atlantis launches for the STS-135 mission to the International Space Station in the final mission of the Space Shuttle Program at NASA?s Kennedy Space Center in Florida. Liftoff was at 11:29 a.m. (EDT) on July 8, 2011. Onboard are NASA astronauts Chris Ferguson, STS-135 commander; Doug Hurley, pilot; Sandy Magnus and Rex Walheim, both mission specialists. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

  9. STS_135_Launch

    NASA Image and Video Library

    2011-07-08

    JSC2011-E-067612 (8 July 2011) --- The space shuttle Atlantis launches for the STS-135 mission to the International Space Station in the final mission of the Space Shuttle Program at NASA?s Kennedy Space Center in Florida. Liftoff was at 11:29 a.m. (EDT) on July 8, 2011. Onboard are NASA astronauts Chris Ferguson, STS-135 commander; Doug Hurley, pilot; Sandy Magnus and Rex Walheim, both mission specialists. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

  10. STS_135_Launch

    NASA Image and Video Library

    2011-07-08

    JSC2011-E-067590 (8 July 2011) --- The space shuttle Atlantis launches for the STS-135 mission to the International Space Station in the final mission of the Space Shuttle Program at NASA?s Kennedy Space Center in Florida. Liftoff was at 11:29 a.m. (EDT) on July 8, 2011. Onboard are NASA astronauts Chris Ferguson, STS-135 commander; Doug Hurley, pilot; Sandy Magnus and Rex Walheim, both mission specialists. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

  11. STS_135_Launch

    NASA Image and Video Library

    2011-07-09

    JSC2011-E-067640 (8 July 2011) --- The space shuttle Atlantis launches for the STS-135 mission to the International Space Station in the final mission of the Space Shuttle Program at NASA?s Kennedy Space Center in Florida. Liftoff was at 11:29 a.m. (EDT) on July 8, 2011. Onboard are NASA astronauts Chris Ferguson, STS-135 commander; Doug Hurley, pilot; Sandy Magnus and Rex Walheim, both mission specialists. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

  12. Comparison of migration rate and survival between radio-tagged and PIT-tagged migrant yearling chinook salmon in the Snake and Columbia rivers

    USGS Publications Warehouse

    Hockersmith, E.E.; Muir, W.D.; Smith, S.G.; Sandford, B.P.; Perry, R.W.; Adams, N.S.; Rondorf, D.W.

    2003-01-01

    A study was conducted to compare the travel times, detection probabilities, and survival of migrant hatchery-reared yearling chinook salmon Oncorhynchus tshawytscha tagged with either gastrically or surgically implanted sham radio tags (with an imbedded passive integrated transponder [PIT] tag) with those of their cohorts tagged only with PIT tags in the Snake and Columbia rivers. Juvenile chinook salmon with gastrically implanted radio tags migrated significantly faster than either surgically radio-tagged or PIT-tagged fish, while migration rates were similar among surgically radio-tagged and PIT-tagged fish. The probabilities of PIT tag detection at downstream dams varied by less than 5% and were not significantly different among the three groups. Survival was similar among treatments for median travel times of less than approximately 6 d (migration distance of 106 km). However, for both gastrically and surgically radio-tagged fish, survival was significantly less than for PIT-tagged fish, for which median travel times exceeded approximately 10 d (migration distance of 225 km). The results of this study support the use of radio tags to estimate the survival of juvenile chinook salmon having a median fork length of approximately 150 mm (range, 127-285 mm) and a median travel time of migration of less than approximately 6 d.

  13. Mission Safety Evaluation Report for STS-43, Postflight Edition

    NASA Technical Reports Server (NTRS)

    Hill, William C.; Finkel, Seymour I.

    1991-01-01

    Some of the topics covered include: (1) an STS-43 mission summary; (2) safety risks factors/issues; (3) resolved STS-43 safety risk factors; (4) STS-40 inflight anomalies; (5) STS-37 inflight anomalies; and (6) STS-43 inflight anomalies. Background information and a list of acronyms are also presented.

  14. STS-113 Crew Training Clip

    NASA Technical Reports Server (NTRS)

    2002-01-01

    The STS-113 crew consists of Commander Jim Weatherbee, Pilot Paul Lockhart, and Mission Specialists Michael Lopez-Alegria and John Herrington. The goal of the STS-113 mission is to deliver the Expedition Six crew to the International Space Station and return the Expedition Five crew to Earth. Also, the P1 Truss will be installed on the International Space Station. The STS-113 crew is shown getting suited for Pre-Launch Ingress and Egress. The Neutral Buoyancy Lab Extravehicular Activity training (NBL) (EVA), CETA Bolt Familiarization, and Photography TV instruction are also presented.

  15. Mission safety evaluation report for STS-38, postflight edition

    NASA Technical Reports Server (NTRS)

    Hill, William C.; Finkel, Seymour I.

    1990-01-01

    After several months delay due to Liquid Hydrogen (LH2) leaks in the External Tank (ET) 17 in LH2 umbilical disconnect, Atlantis was launched at 6:48 p.m. Eastern Standard Time on 15 Nov. 1990. Atlantis was cleared for launch after the replacement of the ET 17 in umbilical disconnect and a successful tanking test on 24 Oct. 1990. No technical concerns were encountered during the countdown. The end of the STS-38 mission was extended by one day because of high crosswinds at the primary landing site, Edwards Air Force Base (EAFB), Calif. On the next day, 20 Nov. 1990, the winds were too high for a safe landing at EAFB, and a decision was made to select the Shuttle Landing Facility (SLF) at the Kennedy Space Center (KSC) at the primary landing site. The deorbit burn was performed on orbit 79 of the STS-38 mission. Reentry and descent were effected with no problems. Postlanding inspection of Atlantis' exterior found little evidence of damage. There has been a concern about returning an Orbiter to KSC, because the last mission to do so experienced tire failure. Atlantis' tires and brakes were in excellent condition with no indication of excessive wear.

  16. A resource of large-scale molecular markers for monitoring Agropyron cristatum chromatin introgression in wheat background based on transcriptome sequences.

    PubMed

    Zhang, Jinpeng; Liu, Weihua; Lu, Yuqing; Liu, Qunxing; Yang, Xinming; Li, Xiuquan; Li, Lihui

    2017-09-20

    Agropyron cristatum is a wild grass of the tribe Triticeae and serves as a gene donor for wheat improvement. However, very few markers can be used to monitor A. cristatum chromatin introgressions in wheat. Here, we reported a resource of large-scale molecular markers for tracking alien introgressions in wheat based on transcriptome sequences. By aligning A. cristatum unigenes with the Chinese Spring reference genome sequences, we designed 9602 A. cristatum expressed sequence tag-sequence-tagged site (EST-STS) markers for PCR amplification and experimental screening. As a result, 6063 polymorphic EST-STS markers were specific for the A. cristatum P genome in the single-receipt wheat background. A total of 4956 randomly selected polymorphic EST-STS markers were further tested in eight wheat variety backgrounds, and 3070 markers displaying stable and polymorphic amplification were validated. These markers covered more than 98% of the A. cristatum genome, and the marker distribution density was approximately 1.28 cM. An application case of all EST-STS markers was validated on the A. cristatum 6 P chromosome. These markers were successfully applied in the tracking of alien A. cristatum chromatin. Altogether, this study provided a universal method of large-scale molecular marker development to monitor wild relative chromatin in wheat.

  17. Reviews of STS Instructional Units.

    ERIC Educational Resources Information Center

    S-STS Reporter, 1987

    1987-01-01

    Provides reviews of modular materials that contain a Science, Technology, Society (STS) theme. Specifies the criteria and distinguishing features of STS materials. Includes reviews of programs which address the topics of energy, genetics, human reproduction, and recombinant DNA research. (ML)

  18. STS-122 Crew Return Ceremony

    NASA Image and Video Library

    2008-02-21

    JSC2008-E-014907 (21 Feb. 2008) --- Johnson Space Center's (JSC) director Michael L. Coats (right) greets astronauts Rex Walheim (left) and Leland Melvin, STS-122 mission specialists, at Ellington Field near JSC prior to the STS-122 crew return ceremonies.

  19. Structural characterization of acylimine-containing blue and red chromophores in mTagBFP and TagRFP fluorescent proteins.

    PubMed

    Subach, Oksana M; Malashkevich, Vladimir N; Zencheck, Wendy D; Morozova, Kateryna S; Piatkevich, Kiryl D; Almo, Steven C; Verkhusha, Vladislav V

    2010-04-23

    We determined the 2.2 A crystal structures of the red fluorescent protein TagRFP and its derivative, the blue fluorescent protein mTagBFP. The crystallographic analysis is consistent with a model in which TagRFP has the trans coplanar anionic chromophore with the conjugated pi-electron system, similar to that of DsRed-like chromophores. Refined conformation of mTagBFP suggests the presence of an N-acylimine functionality in its chromophore and single C(alpha)-C(beta) bond in the Tyr64 side chain. Mass spectrum of mTagBFP chromophore-bearing peptide indicates a loss of 20 Da upon maturation, whereas tandem mass spectrometry reveals that the C(alpha)-N bond in Leu63 is oxidized. These data indicate that mTagBFP has a new type of the chromophore, N-[(5-hydroxy-1H-imidazole-2-yl)methylidene]acetamide. We propose a chemical mechanism in which the DsRed-like chromophore is formed via the mTagBFP-like blue intermediate. (c) 2010 Elsevier Ltd. All rights reserved.

  20. Tag-mediated cooperation with non-deterministic genotype-phenotype mapping

    NASA Astrophysics Data System (ADS)

    Zhang, Hong; Chen, Shu

    2016-01-01

    Tag-mediated cooperation provides a helpful framework for resolving evolutionary social dilemmas. However, most of the previous studies have not taken into account genotype-phenotype distinction in tags, which may play an important role in the process of evolution. To take this into consideration, we introduce non-deterministic genotype-phenotype mapping into a tag-based model with spatial prisoner's dilemma. By our definition, the similarity between genotypic tags does not directly imply the similarity between phenotypic tags. We find that the non-deterministic mapping from genotypic tag to phenotypic tag has non-trivial effects on tag-mediated cooperation. Although we observe that high levels of cooperation can be established under a wide variety of conditions especially when the decisiveness is moderate, the uncertainty in the determination of phenotypic tags may have a detrimental effect on the tag mechanism by disturbing the homophilic interaction structure which can explain the promotion of cooperation in tag systems. Furthermore, the non-deterministic mapping may undermine the robustness of the tag mechanism with respect to various factors such as the structure of the tag space and the tag flexibility. This observation warns us about the danger of applying the classical tag-based models to the analysis of empirical phenomena if genotype-phenotype distinction is significant in real world. Non-deterministic genotype-phenotype mapping thus provides a new perspective to the understanding of tag-mediated cooperation.

  1. STS-104 Crew Photo

    NASA Image and Video Library

    2001-03-09

    STS104-S-002 (May 2001) --- These five astronauts are currently in training for the STS-104 mission, scheduled for a June 2001 liftoff. Seated with the crew insignia are astronauts Steven W. Lindsey (right), mission commander; and Charles O. Hobaugh, pilot. Standing, from left, are astronauts Michael L. Gernhardt, Janet L. Kavandi and James F. Reilly, all mission specialists.

  2. Columbia (STS-50) Landing

    NASA Technical Reports Server (NTRS)

    1992-01-01

    As the orbiter Columbia (STS-50) rolled down Runway 33 of Kennedy Space Center's (KSC) Shuttle Landing Facility, its distinctively colored drag chute deployed to slow down the spaceship. This landing marked OV-102's first end-of-mission landing at KSC and the tenth in the program, and the second shuttle landing with the drag chute. Edwards Air Force Base, CA, was the designated prime for the landing of Mission STS-50, but poor weather necessitated the switch to KSC after a one-day extension of the historic flight. STS-50 was the longest in Shuttle program historyo date, lasting 13 days, 19 hours, 30 minutes and 4 seconds. A crew of seven and the USML-1 were aboard.

  3. STS-119 Crew Walkout

    NASA Image and Video Library

    2009-03-15

    STS119-S-008 (15 March 2009) --- After suiting up, the STS-119 crewmembers pause alongside the Astrovan to wave farewell to onlookers before heading for launch pad 39A for the launch of Space Shuttle Discovery on the STS-119 mission. From the right are astronauts Lee Archambault, commander; Tony Antonelli, pilot; Joseph Acaba, Steve Swanson, Richard Arnold, John Phillips and Japan Aerospace Exploration Agency’s Koichi Wakata, all mission specialists. Wakata will join Expedition 18 in progress to serve as a flight engineer aboard the International Space Station. Discovery will deliver the final pair of power-generating solar array wings and the S6 truss segment. Installation of S6 will signal the station's readiness to house a six-member crew for conducting increased science.

  4. STS-125 Flight Controllers on Console - (Orbit Shift)

    NASA Image and Video Library

    2009-05-11

    JSC2009-E-118888 (11 May 2009) --- Flight director Bryan Lunney monitors data at his console in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center during launch countdown activities a few hundred miles away in Florida, site of Space Shuttle Atlantis? scheduled STS-125 launch to service the Hubble Space Telescope. Liftoff was on time at 2:01 p.m. (EDT) on May 11, 2009 from launch pad 39A at NASA's Kennedy Space Center.

  5. STS-125 Flight Controllers on Console - (Orbit Shift)

    NASA Image and Video Library

    2009-05-11

    JSC2009-E-118822 (11 May 2009) --- Flight director Norm Knight is pictured in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center during launch countdown activities a few hundred miles away in Florida, site of Space Shuttle Atlantis? scheduled STS-125 launch to service the Hubble Space Telescope. Liftoff was on time at 2:01 p.m. (EDT) on May 11, 2009 from launch pad 39A at NASA's Kennedy Space Center.

  6. STS-125 Flight Controllers on Console - (Orbit Shift)

    NASA Image and Video Library

    2009-05-11

    JSC2009-E-118883 (11 May 2009) --- Flight director Tony Ceccacci is pictured in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center during launch countdown activities a few hundred miles away in Florida, site of Space Shuttle Atlantis? scheduled STS-125 launch to service the Hubble Space Telescope. Liftoff was on time at 2:01 p.m. (EDT) on May 11, 2009 from launch pad 39A at NASA's Kennedy Space Center.

  7. STS-125 Flight Controllers on Console - (Orbit Shift)

    NASA Image and Video Library

    2009-05-11

    JSC2009-E-118882 (11 May 2009) --- Flight director Norm Knight is pictured in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center during launch countdown activities a few hundred miles away in Florida, site of Space Shuttle Atlantis? scheduled STS-125 launch to service the Hubble Space Telescope. Liftoff was on time at 2:01 p.m. (EDT) on May 11, 2009 from launch pad 39A at NASA's Kennedy Space Center.

  8. Construction of a chimeric thermostable pyrophosphatase to facilitate its purification and immobilization by using the choline-binding tag.

    PubMed

    Moldes, Cristina; García, José L; García, Pedro

    2004-08-01

    The thermophilic inorganic pyrophosphatase (Pyr) from Thermus thermophilus has been produced in Escherichia coli fused to the C terminus of the choline-binding tag (ChB tag) derived from the choline-binding domain (ChBD) of pneumococcal LytA autolysin. The chimeric ChBD-Pyr protein retains its thermostable activity and can be purified in a single step by DEAE-cellulose affinity chromatography. Pyr can be further released from the ChBD by thrombin, using the specific protease recognition site incorporated in the C terminus of this tag. Remarkably, the ChB tag provides a selective and very strong thermostable noncovalent immobilization of ChBD-Pyr in the DEAE-cellulose matrix. The binding of choline or choline analogues, such as DEAE, confers a high thermal stability to this tag; therefore, the immobilized chimeric enzyme can be assayed at high temperature without protein leakage, demonstrating the usefulness of the ChB tag for noncovalent immobilization of thermophilic proteins. Moreover, ChBD-Pyr can be purified and immobilized in a single step on commercial DEAE-cellulose paper. The affinity of the ChB tag for this versatile solid support can be very helpful in developing many biotechnological applications.

  9. OARE STS-75 (USMP-3) Final Report

    NASA Technical Reports Server (NTRS)

    Rice, James E.

    1996-01-01

    The report is organized into sections representing the phases of work performed in analyzing the STS-75 (USMP-3) results. Section 1 briefly outlines the Orbital Acceleration Research Experiment (OARE) system features, coordinates, and measurement parameters. Section 2 describes the results from STS-75. The mission description, data calibration, and representative data obtained on STS-75 are presented. Also, the anomalous performance of OARE on STS-75 is discussed. Finally, Section 3 presents a discussion of accuracy achieved and achievable with OARE.

  10. Remote Determination of the in situ Sensitivity of a Streckeisen STS-2 Broadband Seismometer

    NASA Astrophysics Data System (ADS)

    Uhrhammer, R. A.; Taira, T.; Hellweg, M.

    2015-12-01

    The sensitivity of a STS-2 broadband seismometer can be determined remotely by two basic methods: 1) via comparison of the inferred ground motions with a reference seismometer, and: 2) via excitation of the calibration coil with a simultaneously recorded stimulus signal. The first method is limited by the accuracy of the reference seismometer and the second method is limited by the accuracy of the motor constant (Gc) of the calibration coil. The accuracy of both methods is also influenced by the signal-to-noise ratio (SNR) in the presence of background seismic noise and the degree of orthogonality of the tri-axial suspension in the STS-2 seismometer. The Streckeisen STS-2 manual states that the signal coil sensitivity (Gs) is 1500 V/(m/s) (+/-1.5%) and it gives Gc to only one decimal place (ie, Gc = 2 g/A). Unfortunately the factory Gc value is not given with sufficient accuracy to be useful for determining the sensitivity of Gs to within 1.5%. Thus we need to determine Gc to enable accurate calibration of the STS-2 via remote excitation of the Gc with a known stimulus. The Berkeley Digital Seismic Network (BDSN) has 12 STS-2 seismometers with co-sited reference sensors (strong motion accelerometers) and they are all recorded by Q330HR data loggers with factory cabling. The procedure is to first verify the sensitivity of the STS-2 signal coils (Gs) via comparison of the ground motions recorded by the STS-2 with the ground motions recorded by the co-sited strong motion accelerometer for an earthquake with has sufficiently high SNR in a passband common to both sensors. The second step in the procedure is to remotely (from Berkeley) excite to calibration coil with a 1 Hz sinusoid which is simultaneously recorded and, using the above measured Gs values, solve for Gc of the calibration coils. The resulting Gc values are typically 2.20-2.50 g/A (accurate to 3+ decimal places) and once the Gc values are found, the STS-2 absolute sensitivity can be determined remotely to

  11. STS-132/ULF4 WFCR Flight Controllers on Console

    NASA Image and Video Library

    2010-05-14

    JSC2010-E-080444 (14 May 2010) --- Flight director Richard Jones is pictured in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center during launch countdown activities a few hundred miles away in Florida, site of space shuttle Atlantis? STS-132 launch. Liftoff was on time at 2:20 p.m. (EDT) on May 14, 2010 from launch pad 39A at NASA's Kennedy Space Center.

  12. Method and apparatus for manufacturing gas tags

    DOEpatents

    Gross, K.C.; Laug, M.T.

    1996-12-17

    For use in the manufacture of gas tags employed in a gas tagging failure detection system for a nuclear reactor, a plurality of commercial feed gases each having a respective noble gas isotopic composition are blended under computer control to provide various tag gas mixtures having selected isotopic ratios which are optimized for specified defined conditions such as cost. Using a new approach employing a discrete variable structure rather than the known continuous-variable optimization problem, the computer controlled gas tag manufacturing process employs an analytical formalism from condensed matter physics known as stochastic relaxation, which is a special case of simulated annealing, for input feed gas selection. For a tag blending process involving M tag isotopes with N distinct feed gas mixtures commercially available from an enriched gas supplier, the manufacturing process calculates the cost difference between multiple combinations and specifies gas mixtures which approach the optimum defined conditions. The manufacturing process is then used to control tag blending apparatus incorporating tag gas canisters connected by stainless-steel tubing with computer controlled valves, with the canisters automatically filled with metered quantities of the required feed gases. 4 figs.

  13. Method and apparatus for manufacturing gas tags

    DOEpatents

    Gross, Kenny C.; Laug, Matthew T.

    1996-01-01

    For use in the manufacture of gas tags employed in a gas tagging failure detection system for a nuclear reactor, a plurality of commercial feed gases each having a respective noble gas isotopic composition are blended under computer control to provide various tag gas mixtures having selected isotopic ratios which are optimized for specified defined conditions such as cost. Using a new approach employing a discrete variable structure rather than the known continuous-variable optimization problem, the computer controlled gas tag manufacturing process employs an analytical formalism from condensed matter physics known as stochastic relaxation, which is a special case of simulated annealing, for input feed gas selection. For a tag blending process involving M tag isotopes with N distinct feed gas mixtures commercially available from an enriched gas supplier, the manufacturing process calculates the cost difference between multiple combinations and specifies gas mixtures which approach the optimum defined conditions. The manufacturing process is then used to control tag blending apparatus incorporating tag gas canisters connected by stainless-steel tubing with computer controlled valves, with the canisters automatically filled with metered quantities of the required feed gases.

  14. Insight into the coordination and the binding sites of Cu(2+) by the histidyl-6-tag using experimental and computational tools.

    PubMed

    Watly, Joanna; Simonovsky, Eyal; Wieczorek, Robert; Barbosa, Nuno; Miller, Yifat; Kozlowski, Henryk

    2014-07-07

    His-tags are specific sequences containing six to nine subsequent histydyl residues, and they are used for purification of recombinant proteins by use of IMAC chromatography. Such polyhistydyl tags, often used in molecular biology, can be also found in nature. Proteins containing histidine-rich domains play a critical role in many life functions in both prokaryote and eukaryote organisms. Binding mode and the thermodynamic properties of the system depend on the specific metal ion and the histidine sequence. Despite the wide application of the His-tag for purification of proteins, little is known about the properties of metal-binding to such tag domains. This inspired us to undertake detailed studies on the coordination of Cu(2+) ion to hexa-His-tag. Experiments were performed using the potentiometric, UV-visible, CD, and EPR techniques. In addition, molecular dynamics (MD) simulations and density functional theory (DFT) calculations were applied. The experimental studies have shown that the Cu(2+) ion binds most likely to two imidazoles and one, two, or three amide nitrogens, depending on the pH. The structures and stabilities of the complexes for the Cu(2+)-Ac-(His)6-NH2 system using experimental and computational tools were established. Polymorphic binding states are suggested, with a possibility of the formation of α-helix structure induced by metal ion coordination. Metal ion is bound to various pairs of imidazole moieties derived from the tag with different efficiencies. The coordination sphere around the metal ion is completed by molecules of water. Finally, the Cu(2+) binding by Ac-(His)6-NH2 is much more efficient compared to other multihistidine protein domains.

  15. STS-79 Mission Insignia

    NASA Technical Reports Server (NTRS)

    1996-01-01

    STS-79 was the fourth in a series of NASA docking missions to the Russian Mir Space Station, leading up to the construction and operation of the International Space Station (ISS). As the first flight of the Spacehab Double Module, STS-79 encompassed research, test and evaluation of ISS, as well as logistics resupply for the Mir Space Station. STS-79 was also the first NASA-Mir American crew member exchange mission, with John E. Blaha (NASA-Mir-3) replacing Shannon W. Lucid (NASA-Mir-2) aboard the Mir Space Station. The lettering of their names either up or down denotes transport up to the Mir Space Station or return to Earth on STS-79. The patch is in the shape of the Space Shuttle's airlock hatch, symbolizing the gateway to international cooperation in space. The patch illustrates the historic cooperation between the United States and Russia in space. With the flags of Russia and the United States as a backdrop, the handshake of Extravehicular Mobility Unit (EMU) which are suited crew members symbolizes mission teamwork, not only of the crew members but also the teamwork between both countries space personnel in science, engineering, medicine and logistics.

  16. Accounting for tagging-to-harvest mortality in a Brownie tag-recovery model by incorporating radio-telemetry data.

    PubMed

    Buderman, Frances E; Diefenbach, Duane R; Casalena, Mary Jo; Rosenberry, Christopher S; Wallingford, Bret D

    2014-04-01

    The Brownie tag-recovery model is useful for estimating harvest rates but assumes all tagged individuals survive to the first hunting season; otherwise, mortality between time of tagging and the hunting season will cause the Brownie estimator to be negatively biased. Alternatively, fitting animals with radio transmitters can be used to accurately estimate harvest rate but may be more costly. We developed a joint model to estimate harvest and annual survival rates that combines known-fate data from animals fitted with transmitters to estimate the probability of surviving the period from capture to the first hunting season, and data from reward-tagged animals in a Brownie tag-recovery model. We evaluated bias and precision of the joint estimator, and how to optimally allocate effort between animals fitted with radio transmitters and inexpensive ear tags or leg bands. Tagging-to-harvest survival rates from >20 individuals with radio transmitters combined with 50-100 reward tags resulted in an unbiased and precise estimator of harvest rates. In addition, the joint model can test whether transmitters affect an individual's probability of being harvested. We illustrate application of the model using data from wild turkey, Meleagris gallapavo, to estimate harvest rates, and data from white-tailed deer, Odocoileus virginianus, to evaluate whether the presence of a visible radio transmitter is related to the probability of a deer being harvested. The joint known-fate tag-recovery model eliminates the requirement to capture and mark animals immediately prior to the hunting season to obtain accurate and precise estimates of harvest rate. In addition, the joint model can assess whether marking animals with radio transmitters affects the individual's probability of being harvested, caused by hunter selectivity or changes in a marked animal's behavior.

  17. Accounting for tagging-to-harvest mortality in a Brownie tag-recovery model by incorporating radio-telemetry data

    USGS Publications Warehouse

    Buderman, Frances E.; Diefenbach, Duane R.; Casalena, Mary Jo; Rosenberry, Christopher S.; Wallingford, Bret D.

    2014-01-01

    The Brownie tag-recovery model is useful for estimating harvest rates but assumes all tagged individuals survive to the first hunting season; otherwise, mortality between time of tagging and the hunting season will cause the Brownie estimator to be negatively biased. Alternatively, fitting animals with radio transmitters can be used to accurately estimate harvest rate but may be more costly. We developed a joint model to estimate harvest and annual survival rates that combines known-fate data from animals fitted with transmitters to estimate the probability of surviving the period from capture to the first hunting season, and data from reward-tagged animals in a Brownie tag-recovery model. We evaluated bias and precision of the joint estimator, and how to optimally allocate effort between animals fitted with radio transmitters and inexpensive ear tags or leg bands. Tagging-to-harvest survival rates from >20 individuals with radio transmitters combined with 50–100 reward tags resulted in an unbiased and precise estimator of harvest rates. In addition, the joint model can test whether transmitters affect an individual's probability of being harvested. We illustrate application of the model using data from wild turkey, Meleagris gallapavo,to estimate harvest rates, and data from white-tailed deer, Odocoileus virginianus, to evaluate whether the presence of a visible radio transmitter is related to the probability of a deer being harvested. The joint known-fate tag-recovery model eliminates the requirement to capture and mark animals immediately prior to the hunting season to obtain accurate and precise estimates of harvest rate. In addition, the joint model can assess whether marking animals with radio transmitters affects the individual's probability of being harvested, caused by hunter selectivity or changes in a marked animal's behavior.

  18. Proximity-Induced Covalent Labeling of Proteins with a Reactive Fluorophore-Binding Peptide Tag.

    PubMed

    Sunbul, Murat; Nacheva, Lora; Jäschke, Andres

    2015-08-19

    Labeling of proteins with fluorescent dyes in live cells enables the investigation of their roles in biological systems by fluorescence microscopy. Because the labeling procedure should not disturb the native function of the protein of interest, it is of high importance to find the optimum labeling method for the problem to be studied. Here, we developed a rapid one-step method to covalently and site-specifically label proteins with a TexasRed fluorophore in vitro and in live bacteria. To this end, a genetically encodable TexasRed fluorophore-binding peptide (TR512) was converted into a reactive tag (ReacTR) by adjoining a cysteine residue which rapidly reacts with N-α-chloroacetamide-conjugated TexasRed fluorophore owing to the proximity effect; ReacTR tag first binds to the TexasRed fluorophore and this interaction brings the nucleophilic cysteine and the electrophilic N-α-chloroacetamide groups in close proximity. Our method has several advantages over existing methods: (i) it utilizes a peptide tag much smaller than fluorescent proteins, the SNAP, CLIP, or HaLo tags; (ii) it allows for labeling of proteins with a small, photostable, red-emitting TexasRed fluorophore; (iii) the probe used is very easy to synthesize; (iv) no enzyme is required to transfer the fluorophore to the peptide tag; and (v) labeling yields a stable covalent product in a very fast reaction.

  19. Space Shuttle Pad Exposure Period Meteorological Parameters STS-1 Through STS-107

    NASA Technical Reports Server (NTRS)

    Overbey, B. G.; Roberts, B. C.

    2005-01-01

    During the 113 missions of the Space Transportation System (STS) to date, the Space Shuttle fleet has been exposed to the elements on the launch pad for approx. 4,195 days. The Natural Environments Branch at Marshall Space Flight Center archives atmospheric environments to which the Space Shuttle vehicles are exposed. This Technical Memorandum (TM) provides a summary of the historical record of the meteorological conditions encountered by the Space Shuttle fleet during the pad exposure period. Parameters included in this TM are temperature, relative humidity, wind speed, wind direction, sea level pressure, and precipitation. Extremes for each of these parameters for each mission are also summarized. Sources for the data include meteorological towers and hourly surface observations. Data are provided from the first launch of the STS in 1981 through the launch of STS-107 in 2003.

  20. The rescue and evaluation of FLAG and HIS epitope-tagged Asia 1 type foot-and-mouth disease viruses.

    PubMed

    Yang, Bo; Yang, Fan; Zhang, Yan; Liu, Huanan; Jin, Ye; Cao, Weijun; Zhu, Zixiang; Zheng, Haixue; Yin, Hong

    2016-02-02

    The VP1 G-H loop of the foot-and-mouth disease virus (FMDV) contains the primary antigenic site, as well as an Arg-Gly-Asp (RGD) binding motif for the αv-integrin family of cell surface receptors. We anticipated that introducing a foreign epitope tag sequence downstream of the RGD motif would be tolerated by the viral capsid and would not destroy the antigenic site of FMDV. In this study, we have designed, generated, and characterized two recombinant FMDVs with a FLAG tag or histidine (HIS) inserted in the VP1 G-H loop downstream of the RGD motif +9 position. The tagged viruses were genetically stable and exhibited similar growth properties with their parental virus. What is more, the recombinant viruses rFMDV-FLAG and rFMDV-HIS showed neutralization sensitivity to FMDV type Asia1-specific mAbs, as well as to polyclonal antibodies. Additionally, the r1 values of the recombinant viruses were similar to that of the parental virus, indicating that the insertion of FLAG or HIS tag sequences downstream of the RGD motif +9 position do not eradicate the antigenic site of FMDV and do not affect its antigenicity. These results indicated that the G-H loop of Asia1 FMDV is able to effectively display the foreign epitopes, making this a potential approach for novel FMDV vaccines development. Copyright © 2015 Elsevier B.V. All rights reserved.

  1. STS-131 Hatch Opening

    NASA Image and Video Library

    2010-04-07

    ISS023-E-020629 (7 April 2010) --- Russian cosmonaut Oleg Kotov (far left), Expedition 23 commander, prepares to greet the STS-131 crew as it comes aboard the International Space Station to spend several days in a number of busy joint activities for the two crews. Seen clearly in the hatch are astronaut Alan Poindexter, STS-131 commander, and Japan Aerospace Agency astronaut Naoko Yamazaki, mission specialist.

  2. STS-64 SAFER Assembly

    NASA Image and Video Library

    1993-12-10

    S93-50137 (December 1993) --- This small mobility-aiding back harness, complemented in extravehicular activity (EVA) with a hand controller unit and called the Simplified Aid for EVA Rescue (SAFER) system, will get extensive in-space evaluation and testing during the STS-64 mission. In this view the SAFER is open to reveal the gas supply and thrusters. SAFER is to fly on STS-76 as well.

  3. Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis.

    PubMed

    Chen, Jia-Liang; Zhao, Yu; Gong, Yan-Jun; Pan, Bin-Bin; Wang, Xiao; Su, Xun-Cheng

    2018-02-01

    Organic synthesis of a ligand with high binding affinities for paramagnetic lanthanide ions is an effective way of generating paramagnetic effects on proteins. These paramagnetic effects manifested in high-resolution NMR spectroscopy are valuable dynamic and structural restraints of proteins and protein-ligand complexes. A paramagnetic tag generally contains a metal chelating moiety and a reactive group for protein modification. Herein we report two new DTPA-like tags, 4PS-PyDTTA and 4PS-6M-PyDTTA that can be site-specifically attached to a protein with a stable thioether bond. Both protein-tag adducts form stable lanthanide complexes, of which the binding affinities and paramagnetic tensors are tunable with respect to the 6-methyl group in pyridine. Paramagnetic relaxation enhancement (PRE) effects of Gd(III) complex on protein-tag adducts were evaluated in comparison with pseudocontact shift (PCS), and the results indicated that both 4PS-PyDTTA and 4PS-6M-PyDTTA tags are rigid and present high-quality PREs that are crucially important in elucidation of the dynamics and interactions of proteins and protein-ligand complexes. We also show that these two tags are suitable for in-situ protein NMR analysis.

  4. "Astronaut STS-123 EVA 1, NBL Training"

    NASA Image and Video Library

    2007-04-18

    • Event (Mission for flight / Class for training): STS-118 (13A.1) • Title: STS-118 EVA 1 NBL • Date: 4-18-07 • Location: NBL • Key words: 118, 13A.1, S5, NBL • Description: NBL underwater photos of STS-118 EVA 1 S5 install.

  5. Tagging as a Social Literacy Practice

    ERIC Educational Resources Information Center

    MacGillivray, Laurie; Curwen, Margaret Sauceda

    2007-01-01

    Tagging is not simply an act of vandalism or violence; it is a social practice with its own rules and codes--a literacy practice imbued with intent and meaning. Three aspects of tagging reflect its nature as a literate practice: (1) The purpose of tagging to achieve particular social goals and group affiliations; (2) The role of talent to be…

  6. STS_135_ MEDIA

    NASA Image and Video Library

    2011-06-30

    JSC2011-E-060794 (30 June 2011) --- NASA astronaut Chris Ferguson, STS-135 commander, points to acknowledge a reporter while greeting the media along with NASA astronauts Doug Hurley, pilot, and Sandy Magnus and Rex Walheim, both mission specialists, during the STS-135 crew media briefing at NASA?s Johnson Space Center June 30, 2011. The press conference provided the last scheduled opportunity for a large group of press to speak with the crew before the final launch on July 8. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

  7. Quantitative chemical tagging, stellar ages and the chemo-dynamical evolution of the Galactic disc

    NASA Astrophysics Data System (ADS)

    Mitschang, A. W.; De Silva, G.; Zucker, D. B.; Anguiano, B.; Bensby, T.; Feltzing, S.

    2014-03-01

    The early science results from the new generation of high-resolution stellar spectroscopic surveys, such as Galactic Archaeology with HERMES (GALAH) and the Gaia European Southern Observatory survey (Gaia-ESO), will represent major milestones in the quest to chemically tag the Galaxy. Yet this technique to reconstruct dispersed coeval stellar groups has remained largely untested until recently. We build on previous work that developed an empirical chemical tagging probability function, which describes the likelihood that two field stars are conatal, that is, they were formed in the same cluster environment. In this work, we perform the first ever blind chemical tagging experiment, i.e. tagging stars with no known or otherwise discernible associations, on a sample of 714 disc field stars with a number of high-quality high-resolution homogeneous metal abundance measurements. We present evidence that chemical tagging of field stars does identify coeval groups of stars, yet these groups may not represent distinct formation sites, e.g. as in dissolved open clusters, as previously thought. Our results point to several important conclusions, among them that group finding will be limited strictly to chemical abundance space, e.g. stellar ages, kinematics, colours, temperature and surface gravity do not enhance the detectability of groups. We also demonstrate that in addition to its role in probing the chemical enrichment and kinematic history of the Galactic disc, chemical tagging represents a powerful new stellar age determination technique.

  8. STS-120 Crew Portrait

    NASA Technical Reports Server (NTRS)

    2007-01-01

    These seven astronauts took a break from training to pose for the STS-120 crew portrait. Pictured from the left are astronauts Scott E. Parazynski, Douglas H. Wheelock, Stephanie D. Wilson, all mission specialists; George D. Zamka, pilot; Pamela A. Melroy, commander; Daniel M. Tani, Expedition 16 flight engineer; and Paolo A. Nespoli, mission specialist representing the European Space Agency (ESA). The crew members were attired in training versions of their shuttle launch and entry suits. Tani joined Expedition 16 as flight engineer after launching to the International Space Station (ISS) and is scheduled to return home on mission STS-122. STS-120 launched October 23, 2007 with the main objectives of installing the U.S. Node 2, Harmony, and the relocation and deployment of the P6 truss to its permanent location.

  9. STS-119 Crew Walkout

    NASA Image and Video Library

    2009-03-15

    STS119-S-007 (15 March 2009) --- After suiting up, the STS-119 crewmembers exit the Operations and Checkout Building to board the Astrovan, which will take them to launch pad 39A for the launch of Space Shuttle Discovery on the STS-119 mission. From the left are astronauts John Phillips, Japan Aerospace Exploration Agency’s Koichi Wakata, Steve Swanson, Richard Arnold, all mission specialists; Tony Antonelli, pilot; Joseph Acaba (behind Antonelli), mission specialist; and Lee Archambault, commander. Wakata will join Expedition 18 in progress to serve as a flight engineer aboard the International Space Station. Discovery will deliver the final pair of power-generating solar array wings and the S6 truss segment. Installation of S6 will signal the station's readiness to house a six-member crew for conducting increased science.

  10. OARE STS-78 (LMS-1) Final Report

    NASA Technical Reports Server (NTRS)

    Rice, James E.

    1996-01-01

    The report is organized into sections representing the phases of work performed in analyzing the STS-78 (LMS-1) results. Section 1 briefly outlines the Orbital Acceleration Research Experiment (OARE) system features, coordinates, and measurement parameters. Section 2 describes the results from STS-78. The mission description, data calibration, and representative data obtained on STS-78 are presented. Also, the anomalous performance of OARE on STS-78 is discussed. Finally, Section 3 presents a discussion of accuracy achieved and achievable with OARE. Appendix A discusses the data processing methodology in detail.

  11. Teacher in Space - STS-33/51L

    NASA Image and Video Library

    1985-09-12

    Views of STS-33/51L crew personnel Prime McAuliffe and Backup Morgan at the ILC Facility during clothing selection and Building #37 during food sampling, and with the rest of the STS-33/51L crew. 40074: "Teacher-in-Space" Participant Barbara Morgan (right) is briefed on her suit and on personal hygiene equipment to be used on the STS-51L Mission. 1. JSC - Education Program (Teacher in Space) 2. Barbara Morgan 3. Christa McAuliffe 4. STS-33/51L - Crew Training (Uniforms/Food/Crew)

  12. STS-71 preflight crew portrait

    NASA Technical Reports Server (NTRS)

    1995-01-01

    Crew members for the STS-71 mission and the related Mir missions assembled for a crew portrait at JSC. In front are, left to right, Vladimir N. Dezhurov, Robert L. Gibson and Anatoliy Y. Solovyev, mission commanders for Mir-18, STS-71 and Mir-19, respecti

  13. Properties of GluR3 receptors tagged with GFP at the amino or carboxyl terminus

    PubMed Central

    Limon, Agenor; Reyes-Ruiz, Jorge Mauricio; Eusebi, Fabrizio; Miledi, Ricardo

    2007-01-01

    Anatomical visualization of neurotransmitter receptor localization is facilitated by tagging receptors, but this process can alter their functional properties. We have evaluated the distribution and properties of WT glutamate receptor 3 (GluR3) α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors (WT GluR3) and two receptors in which GFP was tagged to the amino terminus (GFP-GluR3) or to the carboxyl terminus (GluR3-GFP). Although the fluorescence in Xenopus oocytes was stronger in the vegetal hemisphere because of localization of internal structures (probable sites of production, storage or recycling of receptors), the insertion of receptors into the plasma membrane was polarized to the animal hemisphere. The fluorescence intensity of oocytes injected with GluR3-GFP RNA was approximately double that of oocytes injected with GFP-GluR3 RNA. Accordingly, GluR3-GFP oocytes generated larger kainate-induced currents than GFP-GluR3 oocytes, with similar EC50 values. Currents elicited by glutamate, or AMPA coapplied with cyclothiazide, were also larger in GluR3-GFP oocytes. The glutamate- to kainate-current amplitude ratios differed, with GluR3-GFP being activated more efficiently by glutamate than the WT or GFP-GluR3 receptors. This pattern correlates with the slower decay of glutamate-induced currents generated by GluR3-GFP receptors. These changes were not observed when GFP was tagged to the amino terminus, and these receptors behaved like the WT. The antagonistic effects of 6-nitro-7-sulfamoylbenzo[f]quinoxaline-2,3-dione (NBQX) and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) were not altered in any of the tagged receptors. We conclude that GFP is a useful and convenient tag for visualizing these proteins. However, the effects of different sites of tag insertion on receptor characteristics must be taken into account in assessing the roles played by these receptor proteins. PMID:17881566

  14. Properties of GluR3 receptors tagged with GFP at the amino or carboxyl terminus.

    PubMed

    Limon, Agenor; Reyes-Ruiz, Jorge Mauricio; Eusebi, Fabrizio; Miledi, Ricardo

    2007-09-25

    Anatomical visualization of neurotransmitter receptor localization is facilitated by tagging receptors, but this process can alter their functional properties. We have evaluated the distribution and properties of WT glutamate receptor 3 (GluR3) alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors (WT GluR3) and two receptors in which GFP was tagged to the amino terminus (GFP-GluR3) or to the carboxyl terminus (GluR3-GFP). Although the fluorescence in Xenopus oocytes was stronger in the vegetal hemisphere because of localization of internal structures (probable sites of production, storage or recycling of receptors), the insertion of receptors into the plasma membrane was polarized to the animal hemisphere. The fluorescence intensity of oocytes injected with GluR3-GFP RNA was approximately double that of oocytes injected with GFP-GluR3 RNA. Accordingly, GluR3-GFP oocytes generated larger kainate-induced currents than GFP-GluR3 oocytes, with similar EC(50) values. Currents elicited by glutamate, or AMPA coapplied with cyclothiazide, were also larger in GluR3-GFP oocytes. The glutamate- to kainate-current amplitude ratios differed, with GluR3-GFP being activated more efficiently by glutamate than the WT or GFP-GluR3 receptors. This pattern correlates with the slower decay of glutamate-induced currents generated by GluR3-GFP receptors. These changes were not observed when GFP was tagged to the amino terminus, and these receptors behaved like the WT. The antagonistic effects of 6-nitro-7-sulfamoylbenzo[f]quinoxaline-2,3-dione (NBQX) and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) were not altered in any of the tagged receptors. We conclude that GFP is a useful and convenient tag for visualizing these proteins. However, the effects of different sites of tag insertion on receptor characteristics must be taken into account in assessing the roles played by these receptor proteins.

  15. STS-61 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W., Jr.

    1994-01-01

    The STS-61 Space Shuttle Program Mission Report summarizes the Hubble Space Telescope (HST) servicing mission as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Redesigned Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSME) systems performance during the fifty-ninth flight of the Space Shuttle Program and fifth flight of the Orbiter vehicle Endeavour (OV-105). In addition to the Orbiter, the flight vehicle consisted of an ET designated as ET-60; three SSME's which were designated as serial numbers 2019, 2033, and 2017 in positions 1, 2, and 3, respectively; and two SRB's which were designated BI-063. The RSRM's that were installed in each SRB were designated as 360L023A (lightweight) for the left SRB, and 360L023B (lightweight) for the right SRB. This STS-61 Space Shuttle Program Mission Report fulfills the Space Shuttle Program requirement as documented in NSTS 07700, Volume 8, Appendix E. That document requires that each major organizational element supporting the Program report the results of its hardware evaluation and mission performance plus identify all related in-flight anomalies. The primary objective of the STS-61 mission was to perform the first on-orbit servicing of the Hubble Space Telescope. The servicing tasks included the installation of new solar arrays, replacement of the Wide Field/Planetary Camera I (WF/PC I) with WF/PC II, replacement of the High Speed Photometer (HSP) with the Corrective Optics Space Telescope Axial Replacement (COSTAR), replacement of rate sensing units (RSU's) and electronic control units (ECU's), installation of new magnetic sensing systems and fuse plugs, and the repair of the Goddard High Resolution Spectrometer (GHRS). Secondary objectives were to perform the requirements of the IMAX Cargo Bay Camera (ICBC), the IMAX Camera, and the Air Force Maui Optical Site (AMOS) Calibration Test.

  16. STS-62 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W., Jr.

    1994-01-01

    The STS-62 Space Shuttle Program Mission Report summarizes the Payload activities as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Redesigned Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSHE) systems performance during the sixty-first flight of the Space Shuttle Program and sixteenth flight of the Orbiter vehicle Columbia (OV-102). In addition to the Orbiter, the flight vehicle consisted of an ET designated as ET-62; three SSME's which were designated as serial numbers 2031, 2109, and 2029 in positions 1, 2, and 3, respectively; and two SRB's which were designated BI-064. The RSRM's that were installed in each SRB were designated as 360L036A (lightweight) for the left SRB, and 36OWO36B (welterweight) for the right SRB. This STS-62 Space Shuttle Program Mission Report fulfills the Space Shuttle Program requirement as documented in NSTS 07700, Volume 8, Appendix E. That document requires that each major organizational element supporting the Program report the results of its hardware evaluation and mission performance plus identify all related in-flight anomalies. The primary objectives of the STS-62 mission were to perform the operations of the United States Microgravity Payload-2 (USMP-2) and the Office of Aeronautics and Space Technology-2 (OAST-2) payload. The secondary objectives of this flight were to perform the operations of the Dexterous End Effector (DEE), the Shuttle Solar Backscatter Ultraviolet/A (SSBUV/A), the Limited Duration Space Environment Candidate Material Exposure (LDCE), the Advanced Protein Crystal Growth (APCG), the Physiological Systems Experiments (PSE), the Commercial Protein Crystal Growth (CPCG), the Commercial Generic Bioprocessing Apparatus (CGBA), the Middeck Zero-Gravity Dynamics Experiment (MODE), the Bioreactor Demonstration System (BDS), the Air Force Maui Optical Site Calibration Test (AMOS), and the Auroral Photography Experiment (APE-B).

  17. Surface Acoustic Wave Tag-Based Coherence Multiplexing

    NASA Technical Reports Server (NTRS)

    Youngquist, Robert C. (Inventor); Malocha, Donald (Inventor); Saldanha, Nancy (Inventor)

    2016-01-01

    A surface acoustic wave (SAW)-based coherence multiplexing system includes SAW tags each including a SAW transducer, a first SAW reflector positioned a first distance from the SAW transducer and a second SAW reflector positioned a second distance from the SAW transducer. A transceiver including a wireless transmitter has a signal source providing a source signal and circuitry for transmitting interrogation pulses including a first and a second interrogation pulse toward the SAW tags, and a wireless receiver for receiving and processing response signals from the SAW tags. The receiver receives scrambled signals including a convolution of the wideband interrogation pulses with response signals from the SAW tags and includes a computing device which implements an algorithm that correlates the interrogation pulses or the source signal before transmitting against the scrambled signals to generate tag responses for each of the SAW tags.

  18. 48 CFR 908.7101-7 - Government license tags.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Government license tags... Government license tags. (a) Government license tags shall be procured and assignments recorded by DOE... the District of Columbia, official Government tags shall be obtained from the Department of...

  19. STS-125 Flight Controllers on Console - (Orbit Shift)

    NASA Image and Video Library

    2009-05-11

    JSC2009-E-118817 (11 May 2009) --- Flight controller Mark McDonald monitors data at his console in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center during launch countdown activities a few hundred miles away in Florida, site of Space Shuttle Atlantis? scheduled STS-125 launch to service the Hubble Space Telescope. Liftoff was on time at 2:01 p.m. (EDT) on May 11, 2009 from launch pad 39A at NASA's Kennedy Space Center.

  20. Kuwait Oil Fields as seen from STS-58

    NASA Image and Video Library

    1993-10-20

    STS058-73-054 (18 Oct-1 Nov 1993) --- A clear view of the northern Kuwaiti coast shows the southern part of Kuwait City, and the major oil fields to the south. Oil-laden sands, where wells were set ablaze during the Gulf War in 1991, are visible south of Kuwait City as a dark, elongated patch surrounded by light-colored sand. Oil-stained sand between well sites (dots) and criss-crossing roads is gradually being covered by clean sand carried by strong, seasonal northwest winds.

  1. Earth Observation during STS-113

    NASA Image and Video Library

    2002-12-07

    STS113-332-030 (14 December 2002) --- The STS-113 crewmembers used a 35mm still camera to record this image of Mt. Etna Volcano erupting on the island of Sicily. The south-looking oblique sunset view shows snowy, winter-time Mt. Etna with a brown smutty ash plume.

  2. STS-109 Bailout Training

    NASA Image and Video Library

    2001-08-09

    JSC2001-02185 (9 August 2001) --- Astronaut Duane G. Carey, STS-109 pilot, occupies the pilot’s station during a mission training session in the Space Vehicle Mockup Facility at the Johnson Space Center (JSC). Carey is attired in a training version of the shuttle launch and entry garment. STS-109 will be the 108th shuttle flight and the fourth Hubble Space Telescope (HST) servicing mission.

  3. 48 CFR 908.7101-7 - Government license tags.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Government license tags... Government license tags. (a) Government license tags shall be procured and assignments recorded by DOE... local laws, regulations, and procedures. (d) In the District of Columbia, official Government tags shall...

  4. Landing of Space Shuttle Atlantis / STS-125 Mission

    NASA Image and Video Library

    2009-05-24

    STS125-S-062 (24 May 2009) --- Space Shuttle Atlantis touches down on Runway 22 at Edwards Air Force Base in California, ending the STS-125 mission to repair and upgrade NASA?s Hubble Space Telescope. Onboard are astronauts Scott Altman, commander; Gregory C. Johnson, pilot; Michael Good, Megan McArthur, John Grunsfeld, Mike Massimino and Andrew Feustel, all mission specialists. The main landing gear touched down at 8:39:05 a.m. (PDT) on May 24, 2009. Nose gear touchdown was at 8:39:15 a.m. Wheel-stop was at 8:40:15 a.m., bringing the mission?s elapsed time to 12 days, 21 hours, 37 minutes, 9 seconds. Landing opportunities on May 22, May 23 and May 24 were waved off due to weather concerns at NASA?s Kennedy Space Center in Florida, the shuttle?s primary landing site. Through five spacewalks, the Hubble Space Telescope was refurbished and upgraded with state-of-the-art science instruments that will expand Hubble's capabilities and extend its operational lifespan through at least 2014.

  5. Landing of Space Shuttle Atlantis / STS-125 Mission

    NASA Image and Video Library

    2009-05-24

    STS125-S-064 (24 May 2009) --- Space Shuttle Atlantis approaches landing on Runway 22 at Edwards Air Force Base in California, ending the STS-125 mission to repair and upgrade NASA?s Hubble Space Telescope. Onboard are astronauts Scott Altman, commander; Gregory C. Johnson, pilot; Michael Good, Megan McArthur, John Grunsfeld, Mike Massimino and Andrew Feustel, all mission specialists. The main landing gear touched down at 8:39:05 a.m. (PDT) on May 24, 2009. Nose gear touchdown was at 8:39:15 a.m. Wheel-stop was at 8:40:15 a.m., bringing the mission?s elapsed time to 12 days, 21 hours, 37 minutes, 9 seconds. Landing opportunities on May 22, May 23 and May 24 were waved off due to weather concerns at NASA?s Kennedy Space Center in Florida, the shuttle?s primary landing site. Through five spacewalks, the Hubble Space Telescope was refurbished and upgraded with state-of-the-art science instruments that will expand Hubble's capabilities and extend its operational lifespan through at least 2014.

  6. Landing of Space Shuttle Atlantis / STS-125 Mission

    NASA Image and Video Library

    2009-05-24

    STS125-S-063 (24 May 2009) --- Space Shuttle Atlantis approaches landing on Runway 22 at Edwards Air Force Base in California, ending the STS-125 mission to repair and upgrade NASA?s Hubble Space Telescope. Onboard are astronauts Scott Altman, commander; Gregory C. Johnson, pilot; Michael Good, Megan McArthur, John Grunsfeld, Mike Massimino and Andrew Feustel, all mission specialists. The main landing gear touched down at 8:39:05 a.m. (PDT) on May 24, 2009. Nose gear touchdown was at 8:39:15 a.m. Wheel-stop was at 8:40:15 a.m., bringing the mission?s elapsed time to 12 days, 21 hours, 37 minutes, 9 seconds. Landing opportunities on May 22, May 23 and May 24 were waved off due to weather concerns at NASA?s Kennedy Space Center in Florida, the shuttle?s primary landing site. Through five spacewalks, the Hubble Space Telescope was refurbished and upgraded with state-of-the-art science instruments that will expand Hubble's capabilities and extend its operational lifespan through at least 2014.

  7. STS-132/ULF4 WFCR Flight Controllers on Console

    NASA Image and Video Library

    2010-05-14

    JSC2010-E-080463 (14 May 2010) --- Brent Jett, director, flight crew operations, is pictured in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center during launch countdown activities a few hundred miles away in Florida, site of space shuttle Atlantis' scheduled STS-132 launch. Liftoff was on time at 2:20 p.m. (EDT) on May 14, 2010 from launch pad 39A at NASA's Kennedy Space Center.

  8. STS-132/ULF4 WFCR Flight Controllers on Console

    NASA Image and Video Library

    2010-05-14

    JSC2010-E-080441 (14 May 2010) --- Flight director Richard Jones is pictured at his console in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center during launch countdown activities a few hundred miles away in Florida, site of space shuttle Atlantis? STS-132 launch. Liftoff was on time at 2:20 p.m. (EDT) on May 14, 2010 from launch pad 39A at NASA's Kennedy Space Center.

  9. STS-132/ULF4 WFCR Flight Controllers on Console

    NASA Image and Video Library

    2010-05-14

    JSC2010-E-080454 (14 May 2010) --- Flight director Tony Ceccacci is pictured at his console in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center during launch countdown activities a few hundred miles away in Florida, site of space shuttle Atlantis? STS-132 launch. Liftoff was on time at 2:20 p.m. (EDT) on May 14, 2010 from launch pad 39A at NASA's Kennedy Space Center.

  10. Boundary Layer Transition Results From STS-114

    NASA Technical Reports Server (NTRS)

    Berry, Scott A.; Horvath, Thomas J.; Cassady, Amy M.; Kirk, Benjamin S.; Wang, K. C.; Hyatt, Andrew J.

    2006-01-01

    The tool for predicting the onset of boundary layer transition from damage to and/or repair of the thermal protection system developed in support of Shuttle Return to Flight is compared to the STS-114 flight results. The Boundary Layer Transition (BLT) Tool is part of a suite of tools that analyze the aerothermodynamic environment of the local thermal protection system to allow informed disposition of damage for making recommendations to fly as is or to repair. Using mission specific trajectory information and details of each damage site or repair, the expected time of transition onset is predicted to help determine the proper aerothermodynamic environment to use in the subsequent thermal and stress analysis of the local structure. The boundary layer transition criteria utilized for the tool was developed from ground-based measurements to account for the effect of both protuberances and cavities and has been calibrated against flight data. Computed local boundary layer edge conditions provided the means to correlate the experimental results and then to extrapolate to flight. During STS-114, the BLT Tool was utilized and was part of the decision making process to perform an extravehicular activity to remove the large gap fillers. The role of the BLT Tool during this mission, along with the supporting information that was acquired for the on-orbit analysis, is reviewed. Once the large gap fillers were removed, all remaining damage sites were cleared for reentry as is. Post-flight analysis of the transition onset time revealed excellent agreement with BLT Tool predictions.

  11. Behavioral tagging of extinction learning.

    PubMed

    de Carvalho Myskiw, Jociane; Benetti, Fernando; Izquierdo, Iván

    2013-01-15

    Extinction of contextual fear in rats is enhanced by exposure to a novel environment at 1-2 h before or 1 h after extinction training. This effect is antagonized by administration of protein synthesis inhibitors anisomycin and rapamycin into the hippocampus, but not into the amygdala, immediately after either novelty or extinction training, as well as by the gene expression blocker 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole administered after novelty training, but not after extinction training. Thus, this effect can be attributed to a mechanism similar to synaptic tagging, through which long-term potentiation can be enhanced by other long-term potentiations or by exposure to a novel environment in a protein synthesis-dependent fashion. Extinction learning produces a tag at the appropriate synapses, whereas novelty learning causes the synthesis of plasticity-related proteins that are captured by the tag, strengthening the synapses that generated this tag.

  12. STS-5 crew on middeck

    NASA Image and Video Library

    1982-11-16

    STS005-06-206 (11-16 Nov. 1982) --- Astronaut Joseph P. Allen, STS-5 mission specialist, watches a can of food and a notebook drift on the middeck as Vance C. Brand, commander, (left) and William B. Lenoir, mission specialist, (far left) work at forward middeck lockers. Photo credit: NASA

  13. STS-79 crew insignia

    NASA Image and Video Library

    1998-09-09

    STS79-S-001 (April 1996) --- STS-79 is the fourth in a series of NASA docking missions to the Russian Mir Space Station, leading up to the construction and operation of the International Space Station (ISS). As the first flight of the Spacehab Double Module, STS-79 encompasses research, test and evaluation of ISS, as well as logistics resupply for the Mir Space Station. STS-79 is also the first NASA-Mir American crew member exchange mission, with John E. Blaha (NASA-Mir-3) replacing Shannon W. Lucid (NASA-Mir-2) aboard the Mir Space Station. The lettering of their names either up or down denotes transport up to the Mir Space Station or return to Earth on STS-79. The patch is in the shape of the space shuttle?s airlock hatch, symbolizing the gateway to international cooperation in space. The patch illustrates the historic cooperation between the United States and Russia in space. With the flags of Russia and the United States as a backdrop, the handshake of Extravehicular Mobility Unit (EMU) - suited crew members symbolizes mission teamwork, not only of the crew members but also the teamwork between both countries? space personnel in science, engineering, medicine and logistics. The NASA insignia design for space shuttle flights is reserved for use by the astronauts and for other official use as the NASA Administrator may authorize. Public availability has been approved only in the forms of illustrations by the various news media. When and if there is any change in this policy, which is not anticipated, the change will be publicly announced. Photo credit: NASA

  14. Improved Satellite-Monitored Radio Tags for Large Whales: Dependable ARGOS Location-Only Tags and a GPS-Linked Tag to Reveal 3-Dimensional Body-Orientation and Surface Movements

    DTIC Science & Technology

    2012-09-30

    migration routes and on sperm whales in 2010 and 2011 (funded by BP and NOAA-NRDA) to follow-up on the consequences of the Deepwater Horizon (DWH...dive behavior to especially examine sperm whale foraging behavior. The data will be downloaded from recovered tags to evaluate complex foraging...with the WC Location-only tags off Sakhalin Island, Russia to determine migration routes and tag a small number of sperm whales in the Gulf of Mexico

  15. STS-112 insignia

    NASA Image and Video Library

    2002-03-01

    STS112-S-001 (March 2002) --- The STS-112 emblem symbolizes the ninth assembly mission (9A) to the International Space Station (ISS), a flight which is designed to deliver the Starboard 1 (S1) truss segment. The 30,000 pound truss segment will be lifted to orbit in the payload bay of the space shuttle Atlantis and installed using the ISS robotic arm. Three spacewalks will then be carried out to complete connections between the truss and ISS. Future missions will extend the truss structure to a span of over 350 feet so that it can support the solar arrays and radiators which provide the electrical power and cooling for ISS. The STS-112 emblem depicts ISS from the viewpoint of a departing shuttle, with the installed S1 truss segment outlined in red. A gold trail represents a portion of the shuttle rendezvous trajectory. Where the trajectory meets ISS, a nine-pointed star represents the combined on-orbit team of six shuttle and three ISS crew members who together will complete the S1 truss installation. The trajectory continues beyond the ISS, ending in a six-pointed star representing the Atlantis and the STS-112 crew. The NASA insignia design for space shuttle flights is reserved for use by the astronauts and for other official use as the NASA Administrator may authorize. Public availability has been approved only in the forms of illustrations by the various news media. When and if there is any change in this policy, which is not anticipated, the change will be publicly announced. Photo credit: NASA

  16. sts133-s-133

    NASA Image and Video Library

    2011-03-10

    STS133-S-133 (9 March 2011) --- Space shuttle Discovery rolls down Runway 15 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. Landing was at 11:57 a.m. (EST) on March 9, 2011, completing a more than 12-day STS-133 mission to the International Space Station. Onboard are NASA astronauts Steve Lindsey, commander; Eric Boe, pilot; Steve Bowen, Alvin Drew, Michael Barratt and Nicole Stott, all mission specialists. Discovery and its six-member crew delivered the Permanent Multipurpose Module, packed with supplies and critical spare parts, as well as Robonaut 2, the dexterous humanoid astronaut helper, to the orbiting outpost. STS-133 was Discovery's 39th and final mission. This was the 133rd Space Shuttle Program mission and the 35th shuttle voyage to the space station. Photo credit: NASA or National Aeronautics and Space Administration

  17. Overview of Fusion Tags for Recombinant Proteins.

    PubMed

    Kosobokova, E N; Skrypnik, K A; Kosorukov, V S

    2016-03-01

    Virtually all recombinant proteins are now prepared using fusion domains also known as "tags". The use of tags helps to solve some serious problems: to simplify procedures of protein isolation, to increase expression and solubility of the desired protein, to simplify protein refolding and increase its efficiency, and to prevent proteolysis. In this review, advantages and disadvantages of such fusion tags are analyzed and data on both well-known and new tags are generalized. The authors own data are also presented.

  18. STS-115 crew portrait

    NASA Image and Video Library

    2002-11-08

    STS115-S-002 (8 November 2002) --- These six astronauts take a break from training to pose for the STS-115 crew portrait. Astronauts Brent W. Jett, Jr. (right) and Christopher J. Ferguson, commander and pilot, respectively, flank the mission insignia. The mission specialists are, from left to right, astronauts Heidemarie M. Stefanyshyn-Piper, Joseph R. (Joe) Tanner, Daniel C. Burbank, and Steven G. MacLean, who represents the Canadian Space Agency.

  19. STS_135_FDF

    NASA Image and Video Library

    2011-06-24

    JSC2011-E-059611 (24 June 2011) --- NASA astronaut Sandy Magnus, STS-135 mission specialist, leans out around pilot Doug Hurley, left, to confer with commander Chris Ferguson (out of frame) as the crew of the final space shuttle mission participates in the STS-135 Flight Data File (FDF) review at NASA?s Johnson Space Center June 24, 2011. The review involves examining and annotating more than 200 procedure books and cue cards that comprise all of the detailed technical steps that will be performed during the mission. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

  20. Ear tag

    MedlinePlus

    ... an ear tag or pit are: An inherited tendency to have this facial feature A genetic syndrome ... Elsevier Churchill Livingstone; 2016:chap 19. Review Date 4/24/2017 Updated by: Liora C Adler, MD, ...

  1. Transportation Observations, Considerations, and Recommendations for White Mountain National Forest Provided by the Interagency Transportation Assistance Group (TAG) Plymouth, New Hampshire June 27-29, 2007

    DOT National Transportation Integrated Search

    2007-06-27

    Mountain National Forest by the interagency Transportation Assistance Group (TAG) was conducted June 27-29, 2007, on behalf of the U.S. Forest Service (USFS). This TAG report was prepared subsequent to the site visit and documents the conditions obse...

  2. Evaluation of Tag Attachments on Small Cetaceans

    DTIC Science & Technology

    2013-09-30

    silicon-based antifouling coating, “Propspeed,” as a means to further reduce drag and improve tag performance. Examples of the experimental tags are...the TDR tags, prepared by Wildlife Computers (Figure 1). Half of these were treated with Propspeed antifouling coating, and the other half were left

  3. STS-114: Discovery Day 6 Post MMT Meeting

    NASA Technical Reports Server (NTRS)

    2005-01-01

    Wane Hill, Deputy Manager of the Space Shuttle Program, and Steve Poulos, Manager, Orbiter Project Office discussed damage assessments caused during ascent. Steve further detailed the damage analysis and assessments with chart presentation of composite tile damage and gap filler sites evaluation, protruding ceramic shim, left wing tile damage, comparisons of ground test results versus FD5 focused inspection, and the window 1 blanket. Gap fillers, the STS-73, thermal protection, temperature range, heating and heating range, aerodynamics, aerothermodynamics, risk, foam damage, tile adhesions, and extravehicular activities are topics covered with the News media.

  4. An active site–tail interaction in the structure of hexahistidine-tagged Thermoplasma acidophilum citrate synthase

    PubMed Central

    Murphy, Jesse R.; Donini, Stefano; Kappock, T. Joseph

    2015-01-01

    Citrate synthase (CS) plays a central metabolic role in aerobes and many other organisms. The CS reaction comprises two half-reactions: a Claisen aldol condensation of acetyl-CoA (AcCoA) and oxaloacetate (OAA) that forms citryl-CoA (CitCoA), and CitCoA hydrolysis. Protein conformational changes that ‘close’ the active site play an important role in the assembly of a catalytically competent condensation active site. CS from the thermoacidophile Thermoplasma acidophilum (TpCS) possesses an endogenous Trp fluorophore that can be used to monitor the condensation reaction. The 2.2 Å resolution crystal structure of TpCS fused to a C-terminal hexahistidine tag (TpCSH6) reported here is an ‘open’ structure that, when compared with several liganded TpCS structures, helps to define a complete path for active-site closure. One active site in each dimer binds a neighboring His tag, the first nonsubstrate ligand known to occupy both the AcCoA and OAA binding sites. Solution data collectively suggest that this fortuitous interaction is stabilized by the crystalline lattice. As a polar but almost neutral ligand, the active site–tail interaction provides a new starting point for the design of bisubstrate-analog inhibitors of CS. PMID:26457521

  5. STS pilot user development program

    NASA Technical Reports Server (NTRS)

    Mcdowell, J. R.

    1977-01-01

    Full exploitation of the STS capabilities will be not only dependent on the extensive use of the STS for known space applications and research, but also on new, innovative ideas of use originating with both current and new users. In recognition of this, NASA has been engaged in a User Development Program for the STS. The program began with four small studies. Each study addressed a separate sector of potential new users to identify techniques and methodologies for user development. The collective results established that a user development function was not only feasible, but necessary for NASA to realize the full potential of the STS. This final report begins with a description of the overall pilot program plan, which involved five specific tasks defined in the contract Statement of Work. Each task is then discussed separately; but two subjects, the development of principal investigators and space processing users, are discussed separately for improved continuity of thought. These discussions are followed by a summary of the primary results and conclusions of the Pilot User Development Program. Specific recommendations of the study are given.

  6. Earth observations taken from Space Shuttle Columbia during STS-78 mission

    NASA Image and Video Library

    1996-06-26

    STS078-726-014 (20 June - 7 July 1996) --- Washington D.C., located in the center of the photo, was photographed in both regular color (here) and infrared (STS078-760-010) by the Life and Microgravity Spacelab (LMS-1) crew members. The city of Washington is coextensive with the District of Columbia, whose site was agreed upon by Congress in 1790 as the permanent seat of government for the new nation. It is located at the head of the navigable portion of the Potomac River, which separates it from Virginia to the southwest. It covers an area of 68-square-mile (177-square-kilometers). Andrews Air Force Base is seen east southeast of Washington D.C. Baltimore, Maryland, surrounding the Patapsco River mouth into Chesapeake Bay is located northeast of Washington D. C. or in upper right.

  7. The STS-95 crew participates in a media briefing before returning to JSC

    NASA Technical Reports Server (NTRS)

    1998-01-01

    Media representatives from all over the world fill the Kennedy Space Center Press Site Auditorium for a press conference held by the STS-95 crew before their return to the Johnson Space Center in Houston, Texas. The STS-95 crew members are Mission Commander Curtis L. Brown Jr.; Pilot Steven W. Lindsey; Mission Specialist and Payload Commander Stephen K. Robinson; Mission Specialist Scott E. Parazynski; Mission Specialist Pedro Duque, with the European Space Agency (ESA); Payload Specialist Chiaki Mukai, with the National Space Development Agency of Japan (NASDA); and Payload Specialist John H. Glenn Jr., a senator from Ohio and one of the original seven Project Mercury astronauts. The STS-95 mission ended with landing at Kennedy Space Center's Shuttle Landing Facility at 12:04 p.m. EST on Nov. 7. The mission included research payloads such as the Spartan-201 solar- observing deployable spacecraft, the Hubble Space Telescope Orbital Systems Test Platform, the International Extreme Ultraviolet Hitchhiker, as well as a SPACEHAB single module with experiments on space flight and the aging process.

  8. STS-109 Crew Interview: Grunsfeld

    NASA Technical Reports Server (NTRS)

    2002-01-01

    STS-109 Payload Commander John Grunsfeld is seen during a prelaunch interview answering questions about his inspiration to become an astronaut and his career path. He gives details on the mission's goal (which is to service the Hubble Space Telescope (HST)), his role during the mission, the five scheduled spacewalks, the Columbia Orbiter's recent upgrades, and what he sees as the challenges of the mission. Grunsfeld describes how his experience on the STS-103 mission, a previous HST servicing mission, has helped prepare him for the STS-109 mission. The interview ends with Grunsfeld explaining why the servicing of the Reaction Wheel Assembly, a task added late in his training, is so important.

  9. Discovery STS-131 Mission Landing

    NASA Image and Video Library

    2010-04-20

    STS131-S-092 (20 April 2010) --- Japanese astronaut Naoko Yamazaki, left, and Dr. Kuniaki Shiraki, Executive Director, Japan Aerospace Exploration Agency (JAXA), talk near the space shuttle Discovery shortly after Discovery and the STS-131 crew landed at the Kennedy Space Center in Cape Canaveral, Fla., on April 20, 2010. NASA astronauts Alan Poindexter, commander; James P. Dutton Jr., pilot; Dorothy Metcalf-Lindenburger, Rick Mastracchio, Stephanie Wilson, Clayton Anderson and Japanese astronaut Naoko Yamazaki, all mission specialists, returned from their 15-day journey of more than 6.2 million miles. The STS-131 mission to the International Space Station delivered science racks, new crew sleeping quarters, equipment and supplies. Photo credit: NASA/Bill Ingalls

  10. Discovery STS-131 Mission Landing

    NASA Image and Video Library

    2010-04-20

    STS131-S-091 (20 April 2010) --- NASA Deputy Administrator Lori Garver and NASA astronaut Alan Poindexter, STS-131 commander, walk around under the space shuttle Discovery shortly after Discovery and its seven-member crew landed at the Kennedy Space Center in Cape Canaveral, Fla., on April 20, 2010. Poindexter and NASA astronaut James P. Dutton Jr., pilot; along with NASA astronauts Dorothy Metcalf-Lindenburger, Rick Mastracchio, Stephanie Wilson, Clayton Anderson and Japanese astronaut Naoko Yamazaki, all mission specialists, returned from their 15-day journey of more than 6.2 million miles. The STS-131 mission to the International Space Station delivered science racks, new crew sleeping quarters, equipment and supplies. Photo credit: NASA/Bill Ingalls

  11. PIT-tagged particle study of bed mobility in a Maine salmon river impacted by logging activities

    NASA Astrophysics Data System (ADS)

    Thompson, D. M.; Fixler, S. A.; Roberts, K. E.; McKenna, M.; Marshall, A. E.; Koenig, S.

    2017-12-01

    Presenting an interim report on a study on the Narraguagus River in Maine, which utilizes laser total stations cross-sectional surveys and tracking of passive integrated transponder (PIT) tags embedded in glass spheres to document changes in channel-bed characteristics associated with large wood (LW) additions and natural spawning activities. In 2016, work was initiated to monitor changes in bed elevation and sediment mobility with the addition of LW to the Narraguagus River as part of a restoration effort. Ten cross-sections, spaced 5-m apart, were established and surveyed with a laser total station in each of three different study reaches. The study sites include a control reach, a section with anticipated spawning activities and a site with ongoing LW placement. A grid of 200 glass spheres embedded with PIT tags, with twenty alternating 25-mm and 40-mm size particles equally spaced along each of the ten transects, were placed to serve as point sensors to detect sediment mobilization within each reach. In 2017, the site was revisited to determine if differences in PIT-tagged tracer particle mobilization reflect locations were LW was added and places where Atlantic salmon (Salmo salar) and sea lamprey (Petromyzon marinus) construct spawning redds. The positions of PIT-tagged tracer particles was recorded, but particles were not disturbed or uncovered to permit study of potential reworking of buried tracer particles the following year. Full tracer particle recovery will be determined in 2018 to determine if depths of tracer burial and changes in bed elevation vary among places near redds, LW and main channel locations. The data will be used to determine if salmon redds are preferentially located in either places with greater evidence of sediment reworking or alternatively in stable areas? The study will help determine the degree of bed disruption associated with spawning activities and whether LW placement encourages similar sediment mobilization processes.

  12. STS-114 and Expedition 11 Onboard Group Photo

    NASA Technical Reports Server (NTRS)

    2005-01-01

    The seven crew members of the STS-114 mission and two Expedition 11 crew members gather for a group shot in the Destiny Laboratory of the International Space Station (ISS). From the left (front row) are astronauts Andrew S. W. Thomas, mission specialist (MS); Eileen M. Collins, STS-114 commander; Cosmonaut Sergei K. Kriklev, Expedition 11 commander representing Russia's Federal Space Agency; and John L. Phillips, Expedition 11 NASA Space Station officer and flight engineer. From the left (back row) are astronauts Soichi Noguchi, STS-114 MS, representing the Japan Aerospace Exploration Agency (JAXA); James M. Kelly, STS-114 pilot; and Charles J. Camarda, Wendy B. Lawrence, and Stephen K. Robinson, all STS-114 mission specialists.

  13. STS-132/ULF4 WFCR Flight Controllers on Console

    NASA Image and Video Library

    2010-05-14

    JSC2010-E-080409 (14 May 2010) --- Brent Jett (left), director, flight crew operations; and flight director Norm Knight are pictured in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center during launch countdown activities a few hundred miles away in Florida, site of space shuttle Atlantis' scheduled STS-132 launch. Liftoff was on time at 2:20 p.m. (EDT) on May 14, 2010 from launch pad 39A at NASA's Kennedy Space Center.

  14. STS-132/ULF4 WFCR Flight Controllers on Console

    NASA Image and Video Library

    2010-05-14

    JSC2010-E-080439 (14 May 2010) --- Flight directors Richard Jones and Tony Ceccacci (foreground) monitor data at their console in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center during launch countdown activities a few hundred miles away in Florida, site of space shuttle Atlantis? STS-132 launch. Liftoff was on time at 2:20 p.m. (EDT) on May 14, 2010 from launch pad 39A at NASA's Kennedy Space Center.

  15. STS-132/ULF4 WFCR Flight Controllers on Console

    NASA Image and Video Library

    2010-05-14

    JSC2010-E-080438 (14 May 2010) --- Flight directors Richard Jones and Tony Ceccacci (foreground) monitor data at their console in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center during launch countdown activities a few hundred miles away in Florida, site of space shuttle Atlantis? STS-132 launch. Liftoff was on time at 2:20 p.m. (EDT) on May 14, 2010 from launch pad 39A at NASA's Kennedy Space Center.

  16. The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal

    PubMed Central

    Vergis, James M.; Wiener, Michael C.

    2011-01-01

    Recombinant proteins typically include one or more affinity tags to facilitate purification and/or detection. Expression constructs with affinity tags often include an engineered protease site for tag removal. Like other enzymes, the activities of proteases can be affected by buffer conditions. The buffers used for integral membrane proteins contain detergents, which are required to maintain protein solubility. We examined the detergent sensitivity of six commonly-used proteases (Enterokinase, Factor Xa, Human Rhinovirus 3C Protease, SUMOstar, Tobacco Etch Virus Protease, and Thrombin) by use of a panel of ninety-four individual detergents. Thrombin activity was insensitive to the entire panel of detergents, thus suggesting it as the optimal choice for use with membrane proteins. Enterokinase and Factor Xa were only affected by a small number of detergents, making them good choices as well. PMID:21539919

  17. STS-43 official crew portrait

    NASA Image and Video Library

    1999-11-09

    STS043-S-002 (May 1991) --- These five astronauts have been assigned to fly aboard the Space Shuttle Atlantis for NASA's STS-43 mission. Astronaut John E. Blaha (center) is mission commander. Other crew members are, left to right, astronauts Shannon W. Lucid, James C. Adamson and G. David Low, all mission specialists; and Michael A. Baker, pilot.

  18. STS-130 Nationals Park Visit

    NASA Image and Video Library

    2010-04-20

    NASA STS-130 crew pose with Winter Olympics medalist Apolo Ohno, center, at Nationals Park, Tuesday, April 20, 2010, in Washington. Pictured from left are STS-130 Mission Specialist Robert Benhken, Mission Specialist Nicholas Patrick, Commander George Zamka, Apolo Ohno, Mission Specialist Stephen Robinson, Mission Specialist Kathryn Hire and Pilot Terry Virts. Photo Credit: (NASA/Carla Cioffi)

  19. EVA view taken during STS-102

    NASA Image and Video Library

    2001-03-11

    STS102-312-004 (11 March 2001) --- Astronaut James S. Voss works while anchored to the remote manipulator system (RMS) robot arm on the Space Shuttle Discovery. This extravehicular activity (EVA), on which Voss was joined by astronaut Susan J. Helms (out of frame), was the first of two scheduled STS-102 space walks. The pair, destined to become members of the Expedition Two crew aboard the station later in the mission, rode aboard Discovery into orbit and at the time of this EVA were still regarded as STS-102 mission specialists.

  20. 9 CFR 2.53 - Use of tags.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Use of tags. 2.53 Section 2.53 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.53 Use of tags. Official tags obtained by a dealer...

  1. 9 CFR 2.53 - Use of tags.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Use of tags. 2.53 Section 2.53 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.53 Use of tags. Official tags obtained by a dealer...

  2. 9 CFR 2.53 - Use of tags.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Use of tags. 2.53 Section 2.53 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.53 Use of tags. Official tags obtained by a dealer...

  3. 9 CFR 2.53 - Use of tags.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Use of tags. 2.53 Section 2.53 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.53 Use of tags. Official tags obtained by a dealer...

  4. 9 CFR 2.53 - Use of tags.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Use of tags. 2.53 Section 2.53 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.53 Use of tags. Official tags obtained by a dealer...

  5. An Evaluation of Installation Methods for STS-1 Seismometers

    USGS Publications Warehouse

    Holcomb, L. Gary; Hutt, Charles R.

    1992-01-01

    INTRODUCTION This report documents the results of a series of experiments conducted by the authors at the Albuquerque Seismological Laboratory (ASl) during the spring and summer of 1991; the object of these experiments was to obtain and document quantitative performance comparisons of three methods of installing STS-1 seismometers. Historically, ASL has installed STS-1 sensors by cementing their thick glass base plates to the concrete floor of the vault (see Peterson and Tilgner, 1985, p 44 and Figure 31, p 51 for the details of this installation technique). This installation technique proved to be fairly satisfactory for the China Digital Seismic Network and for several sets of STS-1 sensors installed in other locations since that time. However, the cementing operation is rather labor intensive and the concrete requires a lengthy (about 1 week) curing time during which the sensor installed on it is noisy. In addition it is difficult to assure that all air bubbles have been removed from the interface between the cement and the glass base plate. If air bubbles are present beneath the plate, horizontal sensors can be unacceptably noisy. Moving a sensor installed in this manner requires the purchase of a new glass base plate because the old plate normally can not be removed without breakage. Therefore, this study was undertaken with the aim of developing an improved method of installing STS-1's. The goals were to develop a method which requires less field site labor during the installation and assures a higher quality installation when finished. In addition, the improved installation technique should promote portability. Two alternate installation techniques were evaluated in this study. One method replaces the cement between the base plate and the vault floor with sand. This method has been used in the French Geoscope program and in several IRIS/IDA installations made by the University of California at San Diego (UCSD) and possibly others. It is easily implemented in

  6. Sentiment topic mining based on comment tags

    NASA Astrophysics Data System (ADS)

    Zhang, Daohai; Liu, Xue; Li, Juan; Fan, Mingyue

    2018-03-01

    With the development of e-commerce, various comments based on tags are generated, how to extract valuable information from these comment tags has become an important content of business management decisions. This study takes HUAWEI mobile phone tags as an example using the sentiment analysis and topic LDA mining method. The first step is data preprocessing and classification of comment tag topic mining. And then make the sentiment classification for comment tags. Finally, mine the comments again and analyze the emotional theme distribution under different sentiment classification. The results show that HUAWEI mobile phone has a good user experience in terms of fluency, cost performance, appearance, etc. Meanwhile, it should pay more attention to independent research and development, product design and development. In addition, battery and speed performance should be enhanced.

  7. Harvesting Intelligence in Multimedia Social Tagging Systems

    NASA Astrophysics Data System (ADS)

    Giannakidou, Eirini; Kaklidou, Foteini; Chatzilari, Elisavet; Kompatsiaris, Ioannis; Vakali, Athena

    As more people adopt tagging practices, social tagging systems tend to form rich knowledge repositories that enable the extraction of patterns reflecting the way content semantics is perceived by the web users. This is of particular importance, especially in the case of multimedia content, since the availability of such content in the web is very high and its efficient retrieval using textual annotations or content-based automatically extracted metadata still remains a challenge. It is argued that complementing multimedia analysis techniques with knowledge drawn from web social annotations may facilitate multimedia content management. This chapter focuses on analyzing tagging patterns and combining them with content feature extraction methods, generating, thus, intelligence from multimedia social tagging systems. Emphasis is placed on using all available "tracks" of knowledge, that is tag co-occurrence together with semantic relations among tags and low-level features of the content. Towards this direction, a survey on the theoretical background and the adopted practices for analysis of multimedia social content are presented. A case study from Flickr illustrates the efficiency of the proposed approach.

  8. U.S. Congressmen from Florida Tom Feeney and Dave Weldon at the STS-113 launch

    NASA Technical Reports Server (NTRS)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. - U.S. Congressmen from Florida Tom Feeney (left) and Dave Weldon wait in the VIP viewing site for the STS-113 launch. The launch will carry the Expedition 6 crew to the Station and return the Expedition 5 crew to Earth. The major objective of the mission is delivery of the Port 1 (P1) Integrated Truss Assembly, which will be attached to the port side of the S0 truss. Three spacewalks are planned to install and activate the truss and its associated equipment. Launch of Space Shuttle Endeavour on mission STS-113 is now scheduled for Nov. 23 at 7:50 p.m. EST.

  9. Associated Particle Tagging (APT) in Magnetic Spectrometers

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jordan, David V.; Baciak, James E.; Stave, Sean C.

    2012-10-16

    Summary In Brief The Associated Particle Tagging (APT) project, a collaboration of Pacific Northwest National Laboratory (PNNL), Idaho National Laboratory (INL) and the Idaho State University (ISU)/Idaho Accelerator Center (IAC), has completed an exploratory study to assess the role of magnetic spectrometers as the linchpin technology in next-generation tagged-neutron and tagged-photon active interrogation (AI). The computational study considered two principle concepts: (1) the application of a solenoidal alpha-particle spectrometer to a next-generation, large-emittance neutron generator for use in the associated particle imaging technique, and (2) the application of tagged photon beams to the detection of fissile material via active interrogation.more » In both cases, a magnetic spectrometer momentum-analyzes charged particles (in the neutron case, alpha particles accompanying neutron generation in the D-T reaction; in the tagged photon case, post-bremsstrahlung electrons) to define kinematic properties of the relevant neutral interrogation probe particle (i.e. neutron or photon). The main conclusions of the study can be briefly summarized as follows: Neutron generator: • For the solenoidal spectrometer concept, magnetic field strengths of order 1 Tesla or greater are required to keep the transverse size of the spectrometer smaller than 1 meter. The notional magnetic spectrometer design evaluated in this feasibility study uses a 5-T magnetic field and a borehole radius of 18 cm. • The design shows a potential for 4.5 Sr tagged neutron solid angle, a factor of 4.5 larger than achievable with current API neutron-generator designs. • The potential angular resolution for such a tagged neutron beam can be less than 0.5o for modest Si-detector position resolution (3 mm). Further improvement in angular resolution can be made by using Si-detectors with better position resolution. • The report documents several features of a notional generator design

  10. STS-132 ascent flight control team photo with Flight Director Richard Jones and the STS-132 crew

    NASA Image and Video Library

    2010-06-08

    JSC2010-E-090665 (8 June 2010) --- The members of the STS-132 Ascent flight control team and crew members pose for a group portrait in the space shuttle flight control room in the Mission Control Center at NASA's Johnson Space Center. Flight director Richard Jones (right) and NASA astronaut Ken Ham, STS-132 commander, hold the STS-132 mission logo. Additional crew members pictured are NASA astronauts Tony Antonelli, pilot; along with Garrett Reisman, Piers Sellers, Michael Good and Steve Bowen, all mission specialists. Photo credit: NASA or National Aeronautics and Space Administration

  11. STS-87 Mission Specialist Scott in white room

    NASA Technical Reports Server (NTRS)

    1997-01-01

    STS-87 Mission Specialist Winston Scott is assisted with his ascent and re-entry flight suit in the white room at Launch Pad 39B by Danny Wyatt, NASA quality assurance specialist. STS-87 is the fourth flight of the United States Microgravity Payload and Spartan-201. Scott is scheduled to perform an extravehicular activity spacewalk with Mission Specialist Takao Doi, Ph.D., of the National Space Development Agency of Japan, during STS-87. Scott also performed a spacewalk on the STS-72 mission.

  12. CREW TRAINING - STS-33/51L (ZERO-G)

    NASA Image and Video Library

    1985-11-20

    S85-44835 (20 Nov. 1985) --- This flying human chain represents prime and backup payload specialists for two upcoming STS missions. The group, representing trainees for STS-61C later this year and STS-51L early next year, shared some 40 parabolas in NASA?s KSC-135, ?Zero-G? aircraft on Nov. 20. Left to right are Gerard Magilton, RCA backup payload specialist for STS-61C; Sharon Christa McAuliffe, payload specialist/teacher citizen observer for STS-51L; U.S. Rep. Bill Nelson (D., Florida), scheduled for 61-C; Barbara R. Morgan, backup to McAuliffe; and Robert J. Cenker, RCA payload specialist for 61-C. The photo was taken by Keith Meyers, New York Times. Photo credit: NASA

  13. CREW TRAINING - STS-33/51-L (Zero-G)

    NASA Image and Video Library

    1985-11-20

    S85-44834 (20 Nov. 1985) --- This flying human chain represents prime and backup payload specialists for two upcoming STS missions. The group, representing trainees for STS-61C later this year and STS-51L early next year, shared some 40 parabolas in NASA?s KC-135, ?Zero-G? aircraft on Nov. 20, 1985. Left to right are Gerard Magilton, RCA backup payload specialist for STS-61C; Sharon Christa McAuliffe, payload specialist/teacher citizen observer for STS-51L; U.S. Representative Bill Nelson (D., Florida), scheduled for 61C; Barbara R. Morgan, backup to McAuliffe; and Robert J. Cenker, RCA payload specialist for 61C. The photo was taken by Otis Imboden. Photo credit: NASA

  14. STS-121 Crew Portait

    NASA Image and Video Library

    2006-04-05

    STS121-S-002 (5 April 2006) --- These seven astronauts take a break from training to pose for the STS-121 crew portrait. From the left are astronauts Stephanie D. Wilson, Michael E. Fossum, both mission specialists; Steven W. Lindsey, commander; Piers J. Sellers, mission specialist; Mark E. Kelly, pilot; European Space Agency (ESA) astronaut Thomas Reiter of Germany; and Lisa M. Nowak, both mission specialists. The crewmembers are attired in training versions of their shuttle launch and entry suit.

  15. INFLIGHT (EARTH VIEWS) - STS-2

    NASA Image and Video Library

    1981-11-16

    Onboard views by the STS-2 Crew of the Payload Bay with Office of Space and Terrestrial Applications (OSTA)-1 Payload, the Remote Manipulator System (RMS) and Earth views of: Korea, China and Columbia. Also available in 4x5 BW 1. REAGAN, RONALD PRESIDENT - MOCR (STS-2) JSC, HOUSTON, TX Also available in 4x5 BW, 35 CN, 35 BW

  16. STS, symmetry and post-truth.

    PubMed

    Lynch, Michael

    2017-08-01

    This essay takes up a series of questions about the connection between 'symmetry' in Science and Technology Studies (STS) and 'post-truth' in contemporary politics. A recent editorial in this journal by Sergio Sismondo argues that current discussions of 'post-truth' have little to do with conceptions of 'symmetry' or with concerns about 'epistemic democracy' in STS, while others, such as Steve Fuller and Harry Collins, insist that there are such connections. The present essay discusses a series of questions about the meaning of 'post-truth' and 'symmetry', and the connections of those concepts to each other and to 'epistemic democracy'. The essay ends with a series of other questions about STS and contemporary politics, and an invitation to further discussions.

  17. Communication methods, systems, apparatus, and devices involving RF tag registration

    DOEpatents

    Burghard, Brion J [W. Richland, WA; Skorpik, James R [Kennewick, WA

    2008-04-22

    One technique of the present invention includes a number of Radio Frequency (RF) tags that each have a different identifier. Information is broadcast to the tags from an RF tag interrogator. This information corresponds to a maximum quantity of tag response time slots that are available. This maximum quantity may be less than the total number of tags. The tags each select one of the time slots as a function of the information and a random number provided by each respective tag. The different identifiers are transmitted to the interrogator from at least a subset of the RF tags.

  18. 49 CFR 236.76 - Tagging of wires and interference of wires or tags with signal apparatus.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... with signal apparatus. 236.76 Section 236.76 Transportation Other Regulations Relating to... wires and interference of wires or tags with signal apparatus. Each wire shall be tagged or otherwise so... apparatus. [49 FR 3384, Jan. 26, 1984] Inspections and Tests; All Systems ...

  19. 49 CFR 236.76 - Tagging of wires and interference of wires or tags with signal apparatus.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... with signal apparatus. 236.76 Section 236.76 Transportation Other Regulations Relating to... wires and interference of wires or tags with signal apparatus. Each wire shall be tagged or otherwise so... apparatus. [49 FR 3384, Jan. 26, 1984] Inspections and Tests; All Systems ...

  20. Perfluoro(Methylcyclohexane) Tracer Tagging Test and Demonstration

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sigman, M.E.

    On February 14 and 15, 2000, a demonstration of current perfluorocarbon tagging technology and the future potential of these methods was held at Oak Ridge National Laboratory (ORNL). The demonstration consisted of a brief technical discussion followed by a laboratory demonstration. The laboratory demonstrations included the detection of letters, parcels, briefcases and lockers containing perfluorocarbon-tagged papers. Discrimination between tagged and non-tagged items and between three perfluorocarbon tags was demonstrated along with the detection of perfluorocarbon in a background of non-fluorinated volatile organic solvent. All demonstrations involved real-time detection using a direct sampling ion trap mass spectrometer. The technical results obtainedmore » at ORNL during and in preparation for the demonstration are presented in Appendix 1 to assist Tracer Detection Technology Corp. in further evaluating their position on development and marketing of perfluorocarbon tracer technology.« less

  1. Effects of FlAsH/Tetracysteine (TC) tag on PrP proteolysis and PrPres formation by TC-scanning

    PubMed Central

    Taguchi, Yuzuru; Hohsfield, Lindsay A.; Hollister, Jason R.

    2014-01-01

    The FlAsH/tetracysteine (FlAsH/TC) tag is a powerful tool for fluorescent labeling of proteins. However, even small tags such as FlAsH/TC could alter the behavior of the tagged proteins, especially if the insertion occurs at internal sites. Defining the influence of FlAsH/TC on nearby protein-protein interactions might aid in selecting appropriate positions for internal TC insertions and allow the exploitation of serial FlAsH/TC insertions (TC-scanning) as a probe to characterize sites of protein-protein interaction. To explore this application in the context of substrate-protease interactions, we analyzed the effect of FlAsH/TC insertions on proteolysis of cellular prion protein (PrPsen) in in vitro reactions and generation of the C1 metabolic fragment of PrPsen in live neuroblastoma cells. The influence of FlAsH/TC insertion was evaluated by TC-scanning across the cleavage sites of each protease. The results showed that FlAsH/TC inhibited protease cleavage only within limited ranges of the cleavage sites that varied from about 1 to 6 residues-wide depending on the protease, providing an estimate of the PrP residues interacting with each protease. TC-scanning was also used to probe a different type of protein-protein interaction, the conformational conversion of FlAsH-PrPsen to the prion disease-associated isoform, PrPres. PrP constructs with FlAsH/TC insertions at residues 90–96 but not 97–101 were converted to FlAsH-PrPres, identifying a boundary separating loosely versus compactly folded regions of PrPres. Our observations demonstrate that TC-scanning with the FlAsH/TC tag can be a versatile method for probing protein-protein interactions and folding processes. PMID:23943295

  2. Enhanced UHF RFID tags for drug tracing.

    PubMed

    Catarinucci, Luca; Colella, Riccardo; De Blasi, Mario; Patrono, Luigi; Tarricone, Luciano

    2012-12-01

    Radio Frequency Identification (RFID) technology is playing a crucial role for item-level tracing systems in healthcare scenarios. The pharmaceutical supply chain is a fascinating application context, where RFID can guarantee transparency in the drug flow, supporting both suppliers and consumers against the growing counterfeiting problem. In such a context, the choice of the most adequate RFID tag, in terms of shape, frequency, size and reading range, is crucial. The potential presence of items containing materials hostile to the electromagnetic propagation exasperates the problem. In addition, the peculiarities of the different RFID-based checkpoints make even more stringent the requirements for the tag. In this work, the performance of several commercial UHF RFID tags in each step of the pharmaceutical supply chain has been evaluated, confirming the expected criticality. On such basis, a guideline for the electromagnetic design of new high-performance tags capable to overcome such criticalities has been defined. Finally, driven by such guidelines, a new enhanced tag has been designed, realized and tested. Due to patent pending issues, the antenna shape is not shown. Nevertheless, the optimal obtained results do not lose their validity. Indeed, on the one hand they demonstrate that high performance item level tracing systems can actually be implemented also in critical operating conditions. On the other hand, they encourage the tag designer to follow the identified guidelines so to realize enhanced UHF tags.

  3. Illuminating the Sites of Enterovirus Replication in Living Cells by Using a Split-GFP-Tagged Viral Protein

    PubMed Central

    van der Schaar, H. M.; Melia, C. E.; van Bruggen, J. A. C.; Strating, J. R. P. M.; van Geenen, M. E. D.; Koster, A. J.; Bárcena, M.

    2016-01-01

    ABSTRACT Like all other positive-strand RNA viruses, enteroviruses generate new organelles (replication organelles [ROs]) with a unique protein and lipid composition on which they multiply their viral genome. Suitable tools for live-cell imaging of enterovirus ROs are currently unavailable, as recombinant enteroviruses that carry genes that encode RO-anchored viral proteins tagged with fluorescent reporters have not been reported thus far. To overcome this limitation, we used a split green fluorescent protein (split-GFP) system, comprising a large fragment [strands 1 to 10; GFP(S1-10)] and a small fragment [strand 11; GFP(S11)] of only 16 residues. The GFP(S11) (GFP with S11 fragment) fragment was inserted into the 3A protein of the enterovirus coxsackievirus B3 (CVB3), while the large fragment was supplied by transient or stable expression in cells. The introduction of GFP(S11) did not affect the known functions of 3A when expressed in isolation. Using correlative light electron microscopy (CLEM), we showed that GFP fluorescence was detected at ROs, whose morphologies are essentially identical to those previously observed for wild-type CVB3, indicating that GFP(S11)-tagged 3A proteins assemble with GFP(S1-10) to form GFP for illumination of bona fide ROs. It is well established that enterovirus infection leads to Golgi disintegration. Through live-cell imaging of infected cells expressing an mCherry-tagged Golgi marker, we monitored RO development and revealed the dynamics of Golgi disassembly in real time. Having demonstrated the suitability of this virus for imaging ROs, we constructed a CVB3 encoding GFP(S1-10) and GFP(S11)-tagged 3A to bypass the need to express GFP(S1-10) prior to infection. These tools will have multiple applications in future studies on the origin, location, and function of enterovirus ROs. IMPORTANCE Enteroviruses induce the formation of membranous structures (replication organelles [ROs]) with a unique protein and lipid composition

  4. Illuminating the Sites of Enterovirus Replication in Living Cells by Using a Split-GFP-Tagged Viral Protein.

    PubMed

    van der Schaar, H M; Melia, C E; van Bruggen, J A C; Strating, J R P M; van Geenen, M E D; Koster, A J; Bárcena, M; van Kuppeveld, F J M

    2016-01-01

    Like all other positive-strand RNA viruses, enteroviruses generate new organelles (replication organelles [ROs]) with a unique protein and lipid composition on which they multiply their viral genome. Suitable tools for live-cell imaging of enterovirus ROs are currently unavailable, as recombinant enteroviruses that carry genes that encode RO-anchored viral proteins tagged with fluorescent reporters have not been reported thus far. To overcome this limitation, we used a split green fluorescent protein (split-GFP) system, comprising a large fragment [strands 1 to 10; GFP(S1-10)] and a small fragment [strand 11; GFP(S11)] of only 16 residues. The GFP(S11) (GFP with S11 fragment) fragment was inserted into the 3A protein of the enterovirus coxsackievirus B3 (CVB3), while the large fragment was supplied by transient or stable expression in cells. The introduction of GFP(S11) did not affect the known functions of 3A when expressed in isolation. Using correlative light electron microscopy (CLEM), we showed that GFP fluorescence was detected at ROs, whose morphologies are essentially identical to those previously observed for wild-type CVB3, indicating that GFP(S11)-tagged 3A proteins assemble with GFP(S1-10) to form GFP for illumination of bona fide ROs. It is well established that enterovirus infection leads to Golgi disintegration. Through live-cell imaging of infected cells expressing an mCherry-tagged Golgi marker, we monitored RO development and revealed the dynamics of Golgi disassembly in real time. Having demonstrated the suitability of this virus for imaging ROs, we constructed a CVB3 encoding GFP(S1-10) and GFP(S11)-tagged 3A to bypass the need to express GFP(S1-10) prior to infection. These tools will have multiple applications in future studies on the origin, location, and function of enterovirus ROs. IMPORTANCE Enteroviruses induce the formation of membranous structures (replication organelles [ROs]) with a unique protein and lipid composition specialized for

  5. STS-105 Flight Control Team Photo

    NASA Image and Video Library

    2001-07-31

    JSC2001-02115 (31 July 2001) --- The flight controllers for the Ascent/Entry shift for the upcoming STS-105 mission pose with the assigned astronaut crew for a team portrait in the Shuttle Flight Control Room (WFCR) of Houston's Mission Control Center (MCC). Flight director John Shannon (left center) and STS-105 commander Scott J. Horowitz hold the mission logo. Also pictured on the front row are spacecraft communicator Kenneth D. Cockrell and STS-105 crew members Daniel T. Barry, Frederick W. (Rick) Sturckow and Patrick G. Forrester. The team had been participating in an integrated simulation for the scheduled August mission.

  6. STS-132vesrsion8NASA

    NASA Image and Video Library

    2010-02-03

    STS132-S-001 (February 2010) --- The STS-132 mission will be the 32nd flight of the space shuttle Atlantis. The primary STS-132 mission objective is to deliver the Russian-made MRM-1 (Mini Research Module) to the International Space Station (ISS). Atlantis will also deliver a new communications antenna and a new set of batteries for one of the ISS solar arrays. The STS-132 mission patch features Atlantis flying off into the sunset as the end of the Space Shuttle Program approaches. However the sun is also heralding the promise of a new day as it rises for the first time on a new ISS module, the MRM-1, which is also named ?Rassvet,? the Russian word for dawn. The NASA insignia design for space shuttle flights is reserved for use by the astronauts and for other official use as the NASA Administrator may authorize. Public availability has been approved only in the forms of illustrations by the various news media. When and if there is any change in this policy, which is not anticipated, the change will be publicly announced. Photo credit: NASA

  7. Ordering and interactions between Cl adatoms on Cu(111) and their influence on the local electronic properties as measured by STM and STS

    NASA Astrophysics Data System (ADS)

    Torsney, Samuel; Naydenov, Borislav; Boland, John J.

    2017-12-01

    We present a scanning tunneling microscopy/spectroscopy study of compressed Cl adlayers on Cu(111) under ultrahigh-vacuum conditions. We describe a rational scheme to assign Cl adatoms to different surface sites. The dominant electronic state visible in scanning tunneling spectroscopy (STS) corresponds to an antibonding interaction between the Cl adlayer and the copper surface. This state was observed to be 200 meV higher in energy at hcp sites compared to fcc sites, and it is attributed to the greater charge transfer to Cl adatoms at hcp sites. Although there was no STS signature associated with bridging sites, the presence of bridging Cl adatoms along the periphery of fcc domains caused a shift in the energy of the interface state in the latter. These results shed important light on the ordering and interaction between Cl adatoms on Cu(111) and their influence of the local electronic structure of the surface.

  8. Measuring and Predicting Tag Importance for Image Retrieval.

    PubMed

    Li, Shangwen; Purushotham, Sanjay; Chen, Chen; Ren, Yuzhuo; Kuo, C-C Jay

    2017-12-01

    Textual data such as tags, sentence descriptions are combined with visual cues to reduce the semantic gap for image retrieval applications in today's Multimodal Image Retrieval (MIR) systems. However, all tags are treated as equally important in these systems, which may result in misalignment between visual and textual modalities during MIR training. This will further lead to degenerated retrieval performance at query time. To address this issue, we investigate the problem of tag importance prediction, where the goal is to automatically predict the tag importance and use it in image retrieval. To achieve this, we first propose a method to measure the relative importance of object and scene tags from image sentence descriptions. Using this as the ground truth, we present a tag importance prediction model to jointly exploit visual, semantic and context cues. The Structural Support Vector Machine (SSVM) formulation is adopted to ensure efficient training of the prediction model. Then, the Canonical Correlation Analysis (CCA) is employed to learn the relation between the image visual feature and tag importance to obtain robust retrieval performance. Experimental results on three real-world datasets show a significant performance improvement of the proposed MIR with Tag Importance Prediction (MIR/TIP) system over other MIR systems.

  9. LAUNCH (IGOR) - STS-1

    NASA Image and Video Library

    1981-04-12

    S81-33179 (12 April 1981) --- Though their STS-1 task has been performed, the two solid rocket boosters (SRB) still glow following their jettisoning from the space shuttle Columbia on its way to many firsts. Among the history recorded by the spacecraft is the marking of a mission in a reusable spacecraft. STS-1 is NASA's first manned mission since the Apollo-Soyuz Test Project in 1975. Inside the cabin of the climbing spacecraft are astronauts John W. Young and Robert L. Crippen. Photo credit: NASA

  10. sts093-s-016

    NASA Image and Video Library

    1999-07-27

    STS093-(S)-016 (27 July 1999) --- Members of the STS-93 crew pose in front of the Space Shuttle Columbia following the night landing on runway 33 at Kennedy Space Center's Shuttle Landing Facility. From the left are astronauts Catherine G. (Cady) Coleman and Steven A. Hawley, both mission specialists; Jeffrey S. Ashby, pilot; Eileen M. Collins, mission commander; and Michel Tognini, mission specialist representing France's Centre National d'Etudes Spatiales (CNES). Main gear touchdown occurred at 11:20:35 p.m.(EDT), July 27, 1999.

  11. Oceans apart? Short-term movements and behaviour of adult bull sharks Carcharhinus leucas in Atlantic and Pacific Oceans determined from pop-off satellite archival tagging.

    PubMed

    Brunnschweiler, J M; Queiroz, N; Sims, D W

    2010-10-01

    Adult bull sharks Carcharhinus leucas were monitored with electronic tags to investigate horizontal and vertical movements in the Atlantic and Pacific Oceans. In both locations, C. leucas showed some fidelity to specific coastal areas with only limited horizontal movements away from the tagging sites after tag attachment. Fish tagged in the Bahamas were detected mostly in the upper 20 m of the water column in water 25-26° C, whereas C. leucas tagged in Fiji spent most of their time below 20 m in water usually >26° C. The results highlight the importance of coastal inshore habitats for this species. © 2010 The Authors. Journal compilation © 2010 The Fisheries Society of the British Isles.

  12. Indexing Aids at Corporate Websites: The Use of Robots.txt and META Tags.

    ERIC Educational Resources Information Center

    Drott, M. Carl

    2002-01-01

    This study examine 60 corporate Web sites to see if they provided support for automatic indexing, particularly use of the robots.txt and Meta tags for keywords and description. Discusses the use of Java and cookies and suggests that an increase in indexing aids would improve overall index coverage of the Web. (Author/LRW)

  13. Reversible chemoselective tagging and functionalization of methionine containing peptides.

    PubMed

    Kramer, Jessica R; Deming, Timothy J

    2013-06-07

    Reagents were developed to allow chemoselective tagging of methionine residues in peptides and polypeptides, subsequent bioorthogonal functionalization of the tags, and cleavage of the tags when desired. This methodology can be used for triggered release of therapeutic peptides, or release of tagged protein digests from affinity columns.

  14. STS-62 onboard crew portrait

    NASA Image and Video Library

    1994-03-05

    STS062-17-025 (4-18 March 1994) --- The five veteran astronaut crewmembers for NASA's STS-62 mission pose for the traditional inflight portrait on Columbia's aft flight deck. In front are astronauts John H. Casper (left), mission commander; and Pierre J. Thuot, mission specialist. In the rear are astronauts (left to right) Andrew M. Allen, pilot; Marsha S. Ivins and Charles D. (Sam) Gemar, mission specialists.

  15. Earth Observation during STS-113

    NASA Image and Video Library

    2002-12-03

    STS113-348-012 (3 December 2002) --- The STS-113 crewmembers used a 35mm still camera to record this image of a mid latitude storm system. The counter-clockwise swirl shows that this is a northern hemisphere storm. The storm was northeast of the Mediterranean Sea, covering the Balkans and western Turkey. The view was taken looking northwest in the early afternoon of Dec 3, 2002.

  16. Magnetic vector field tag and seal

    DOEpatents

    Johnston, Roger G.; Garcia, Anthony R.

    2004-08-31

    One or more magnets are placed in a container (preferably on objects inside the container) and the magnetic field strength and vector direction are measured with a magnetometer from at least one location near the container to provide the container with a magnetic vector field tag and seal. The location(s) of the magnetometer relative to the container are also noted. If the position of any magnet inside the container changes, then the measured vector fields at the these locations also change, indicating that the tag has been removed, the seal has broken, and therefore that the container and objects inside may have been tampered with. A hollow wheel with magnets inside may also provide a similar magnetic vector field tag and seal. As the wheel turns, the magnets tumble randomly inside, removing the tag and breaking the seal.

  17. SHUTTLE - PAYLOADS (STS-41G) - KSC

    NASA Image and Video Library

    1984-10-05

    Payload canister transporter in Vertical Processing Facility Clean Room loaded with Earth Radiation Budget Experiment (ERBS), Large Format Camera (LFC), and Orbital Reservicing System (ORS) for STS-41G Mission. 1. STS-41G - EXPERIMENTS 2. CAMERAS - LFC KSC, FL Also available in 4x5 CN

  18. STS-131 Launch from Firing Room 4

    NASA Image and Video Library

    2010-04-05

    STS131-S-050 (5 April 2010) --- NASA commentator Mike Curie and astronaut Kathryn (Kay) Hire discuss the launch of space shuttle Discovery on the STS-131 mission in the Launch Control Center's Firing Room 4 at NASA's Kennedy Space Center in Florida. The seven-member STS-131 crew will deliver the multi-purpose logistics module Leonardo, filled with supplies, a new crew sleeping quarters and science racks that will be transferred to the International Space Station's laboratories. The crew also will switch out a gyroscope on the station’s truss structure, install a spare ammonia storage tank and retrieve a Japanese experiment from the station’s exterior. STS-131 is the 33rd shuttle mission to the station and the 131st shuttle mission overall.

  19. Landing of Space Shuttle Atlantis / STS-125 Mission

    NASA Image and Video Library

    2009-05-24

    STS125-S-065 (24 May 2009) --- Space Shuttle Atlantis? drag chute is deployed as the spacecraft rolls toward wheels stop on Runway 22 at Edwards Air Force Base in California, ending the STS-125 mission to repair and upgrade NASA?s Hubble Space Telescope. Onboard are astronauts Scott Altman, commander; Gregory C. Johnson, pilot; Michael Good, Megan McArthur, John Grunsfeld, Mike Massimino and Andrew Feustel, all mission specialists. The main landing gear touched down at 8:39:05 a.m. (PDT) on May 24, 2009. Nose gear touchdown was at 8:39:15 a.m. Wheel-stop was at 8:40:15 a.m., bringing the mission?s elapsed time to 12 days, 21 hours, 37 minutes, 9 seconds. Landing opportunities on May 22, May 23 and May 24 were waved off due to weather concerns at NASA?s Kennedy Space Center in Florida, the shuttle?s primary landing site. Through five spacewalks, the Hubble Space Telescope was refurbished and upgraded with state-of-the-art science instruments that will expand Hubble's capabilities and extend its operational lifespan through at least 2014.

  20. Clone tag detection in distributed RFID systems

    PubMed Central

    Kamaludin, Hazalila; Mahdin, Hairulnizam

    2018-01-01

    Although Radio Frequency Identification (RFID) is poised to displace barcodes, security vulnerabilities pose serious challenges for global adoption of the RFID technology. Specifically, RFID tags are prone to basic cloning and counterfeiting security attacks. A successful cloning of the RFID tags in many commercial applications can lead to many serious problems such as financial losses, brand damage, safety and health of the public. With many industries such as pharmaceutical and businesses deploying RFID technology with a variety of products, it is important to tackle RFID tag cloning problem and improve the resistance of the RFID systems. To this end, we propose an approach for detecting cloned RFID tags in RFID systems with high detection accuracy and minimal overhead thus overcoming practical challenges in existing approaches. The proposed approach is based on consistency of dual hash collisions and modified count-min sketch vector. We evaluated the proposed approach through extensive experiments and compared it with existing baseline approaches in terms of execution time and detection accuracy under varying RFID tag cloning ratio. The results of the experiments show that the proposed approach outperforms the baseline approaches in cloned RFID tag detection accuracy. PMID:29565982

  1. Clone tag detection in distributed RFID systems.

    PubMed

    Kamaludin, Hazalila; Mahdin, Hairulnizam; Abawajy, Jemal H

    2018-01-01

    Although Radio Frequency Identification (RFID) is poised to displace barcodes, security vulnerabilities pose serious challenges for global adoption of the RFID technology. Specifically, RFID tags are prone to basic cloning and counterfeiting security attacks. A successful cloning of the RFID tags in many commercial applications can lead to many serious problems such as financial losses, brand damage, safety and health of the public. With many industries such as pharmaceutical and businesses deploying RFID technology with a variety of products, it is important to tackle RFID tag cloning problem and improve the resistance of the RFID systems. To this end, we propose an approach for detecting cloned RFID tags in RFID systems with high detection accuracy and minimal overhead thus overcoming practical challenges in existing approaches. The proposed approach is based on consistency of dual hash collisions and modified count-min sketch vector. We evaluated the proposed approach through extensive experiments and compared it with existing baseline approaches in terms of execution time and detection accuracy under varying RFID tag cloning ratio. The results of the experiments show that the proposed approach outperforms the baseline approaches in cloned RFID tag detection accuracy.

  2. STS-121 Mission Patch

    NASA Image and Video Library

    2005-06-01

    STS121-S-001 (June 2005) --- The STS-121 patch depicts the space shuttle docked with the International Space Station (ISS) in the foreground, overlaying the astronaut symbol with three gold columns and a gold star. The ISS is shown in the configuration that it will be in during the STS-121 mission. The background shows the nighttime Earth with a dawn breaking over the horizon. STS-121, ISS mission ULF1.1, is the final Shuttle Return to Flight test mission. This utilization and logistics flight will bring a multipurpose logistics module (MPLM) to the ISS with several thousand pounds of new supplies and experiments. In addition, some new orbital replacement units (ORUs) will be delivered and stowed externally on ISS on a special pallet. These ORUs are spares for critical machinery located on the outside of the ISS. During this mission the crew will also carry out testing of shuttle inspection and repair hardware, as well as evaluate operational techniques and concepts for conducting on-orbit inspection and repair. The NASA insignia design for space shuttle flights is reserved for use by the astronauts and for other official use as the NASA Administrator may authorize. Public availability has been approved only in the forms of illustrations by the various news media. When and if there is any change in this policy, which is not anticipated, the change will be publicly announced. Photo credit: NASA

  3. Movements of Satellite-Tagged Blainville’s Beaked Whales Off the Island of Hawaii

    DTIC Science & Technology

    2009-01-01

    Collective, 218 1⁄2 W. 4th Avenue, Olympia, Washington 98501, USA 2NOAA, Northwest Fisheries Science Center, 2725 Montlake Blvd E., Seattle, Washington...site of frequent sonar activity and was to be the site of choke point exer- cises during the 2006 Rim of the Pacific exercise. To date, no mass...Orcinus orca (Andrews et al. 2008). In 2006, satellite tags were deployed on 3 Blainville’s beaked whales, and, in 2008, we were able to deploy

  4. ODS/Mir hatch opening during STS-89 mission

    NASA Image and Video Library

    2009-09-22

    STS089-349-021 (22-31 Jan 1998) --- Cosmonaut Pavel Vinogradov, flight engineer for the Mir-24 crew, peers through the hatch, from the inside of the Russia?s Mir Space Station, at arriving Space Shuttle Endeavour members of the STS-89 crew prior to hatch opening. STS-89/Mir-24 marked the eighth of nine Shuttle/Mir dockings.

  5. STS-95 Payload Specialist Glenn participates in a media briefing before returning to JSC

    NASA Technical Reports Server (NTRS)

    1998-01-01

    STS-95 Payload Specialist John H. Glenn Jr., a senator from Ohio and one of the original seven Project Mercury astronauts, participates in a media briefing at the Kennedy Space Center Press Site Auditorium before returning to the Johnson Space Center in Houston, Texas. The STS-95 mission ended with landing at Kennedy Space Center's Shuttle Landing Facility at 12:04 p.m. EST on Nov. 7. Also participating in the briefing were the other STS-95 crew members: Mission Commander Curtis L. Brown Jr.; Pilot Steven W. Lindsey; Mission Specialist and Payload Commander Stephen K. Robinson; Mission Specialist Scott E. Parazynski; Mission Specialist Pedro Duque, with the European Space Agency (ESA); and Payload Specialist Chiaki Mukai, with the National Space Development Agency of Japan (NASDA). The mission included research payloads such as the Spartan-201 solar-observing deployable spacecraft, the Hubble Space Telescope Orbital Systems Test Platform, the International Extreme Ultraviolet Hitchhiker, as well as a SPACEHAB single module with experiments on space flight and the aging process.

  6. STS-128 crew visits Stennis

    NASA Technical Reports Server (NTRS)

    2009-01-01

    Astronauts C.J. Sturckow (seated, left) and Pat Forrester (seated, right) sign autographs during their Oct. 7 visit to Stennis Space Center. The astronauts visited the rocket engine testing facility to thank Stennis employees for contributions to their recent STS-128 space shuttle mission. All three of the main engines used on the mission were tested at Stennis. Sturckow served as commander for the STS-128 flight; Forrester was a mission specialist. During a 14-day mission aboard space shuttle discovery, the STS-128 crew delivered equipment and supplies to the International Space Station, including science and storage racks, a freezer to store research samples, a new sleeping compartment and an exercise treadmill. The mission featured three spacewalks to replace experiments and install new equipment at the space station.

  7. STS-116 and Expedition 14 crew portrait

    NASA Image and Video Library

    2006-12-19

    ISS014-E-10265 (19 Dec. 2006) --- The Expedition 14 and STS-116 crewmembers gather for a group portrait in the Destiny laboratory of the International Space Station while Space Shuttle Discovery was docked with the station. From the left (front row) are astronauts William A. (Bill) Oefelein, STS-116 pilot; Joan E. Higginbotham and the European Space Agency's (ESA) Thomas Reiter, both STS-116 mission specialists. From the left (center row) are astronauts Mark L. Polansky, STS-116 commander; Robert L. Curbeam Jr., Nicholas J. M. Patrick and European Space Agency's (ESA) Christer Fuglesang, all STS-116 mission specialists. From the left (back row) are cosmonaut Mikhail Tyurin, Expedition 14 flight engineer representing Russia's Federal Space Agency; astronauts Michael E. Lopez-Alegria, Expedition 14 commander and NASA space station science officer; and Sunita L. Williams, Expedition flight engineer. Shortly after the two spacecraft docked on Dec. 11, Williams became a member of the station crew. At the same time, Reiter became a Discovery crewmember for his ride home, completing about six months in space.

  8. A Radio Tag for Big Whales

    ERIC Educational Resources Information Center

    Watkins, William A.

    1978-01-01

    Radio tags to track wildlife have been used for years. However, such tagging of whales has been more complicated and less successful. This article explores the latest technology that is designed to give information over a long period of time. (MA)

  9. POS-Tagging for informal language (study in Indonesian tweets)

    NASA Astrophysics Data System (ADS)

    Suryawati, Endang; Munandar, Devi; Riswantini, Dianadewi; Fatchuttamam Abka, Achmad; Arisal, Andria

    2018-03-01

    This paper evaluates Part-of-Speech Tagging for the formal Indonesian language can be used for the tagging process of Indonesian tweets. In this study, we add five additional tags which reflect to social media attributes to the existing original tagset. Automatic POS tagging process is done by stratified training process with 1000, 1600, and 1800 of annotated tweets. It shows that the process can achieve up to 66.36% accuracy. The experiment with original tagset gives slightly better accuracy (67.39%) than the experiment with five additional tags, but will lose important informations which given by the five additional tagset.POS-Tagging for Informal Language (Study in Indonesian Tweets).

  10. Dispersal and dam passage of sonic-tagged juvenile lake sturgeon in the upper Tennessee River

    USGS Publications Warehouse

    Collier, William R.; Bettoli, Phillip William; Scholten, George D.

    2011-01-01

    More than 90,000 state-endangered lake sturgeon (Acipenser fulvescens) have been stocked into the French Broad River, Holston River, and Fort Loudoun Lake in the upper Tennessee River system. Although incidental reports of anglers catching these fish have increased, little is known about their fate after stocking. Therefore, this study was conducted to evaluate lake sturgeon dispersal throughout the system. Seven submersible ultrasonic receivers were deployed in the upper Tennessee River system and 37 juvenile fish (mean fork length = 660 mm) were surgically implanted with ultrasonic transmitters in the fall of 2007. These fish were stocked at two sites in the headwaters of Fort Loudoun Lake. The receivers logged 1,345 detections of tagged fish and manual tracking located 32 of the 37 tagged lake sturgeon over 21 months. Ten (31%) tagged fish passed through Fort Loudoun Dam and were located downstream in the headwaters of Watts Bar Reservoir; the other tagged fish were still at large above Fort Loudoun Dam when tracking ceased in 2009. Of all fish stocked, three (9%) were last located in the French Broad River and none were located in the Holston River.

  11. STS-86 Mission Specialist Wolf at SLF for TCDT

    NASA Technical Reports Server (NTRS)

    1997-01-01

    STS-86 Mission Specialist David A. Wolf arrives in a T-38 jet at KSCs Shuttle Landing Facility for the Terminal Countdown Demonstration Test (TCDT), a dress rehearsal for launch. STS-86 will be the seventh docking of the Space Shuttle with the Russian Space Station Mir. During the mission, Wolf will transfer to the Mir 24 crew, replacing astronaut C. Michael Foale, who will return to Earth with the rest of the STS-86 crew. Wolf is scheduled to remain on the Mir until his replacement arrives on the STS-89 mission in January. STS-86 is targeted for a Sept. 25 launch aboard the Space Shuttle Atlantis.

  12. sts106-s-005

    NASA Image and Video Library

    2000-09-08

    STS106-S-005 (8 September 2000) --- This distant view of the STS-106 liftoff was photographed across marsh waters around Mosquito Lagoon and Banana Creek, looking toward the Atlantic Ocean. The perfect on-time liftoff of the Space Shuttle Atlantis occurred at 8:45:47 a.m. (EDT), September 8, 2000. Onboard the shuttle were astronauts Terrence W. Wilcutt, Scott D. Altman, Edward T. Lu, Richard A. Mastracchio and Daniel C. Burbank, along with cosmonauts Yuri I. Malenchenko and Boris V. Morukov who represent the Russian Aviation and Space Agency.

  13. sts093-s-013

    NASA Image and Video Library

    1999-06-27

    STS093-S-013 (27 June 1999) --- In this fish-eye view, the Chandra X-ray Observatory rests inside the payload bay of the Space Shuttle Columbia at the Kennedy Space Center (KSC). Chandra is the primary payload on the STS-93 mission, scheduled to launch next month. The world's most powerful X-ray telescope, Chandra, will allow scientists from around the world to see previously invisible black holes and high-temperature gas clouds, giving the observatory the potential to rewrite the books on the structure and evolution of the universe.

  14. STS098-S-002

    NASA Image and Video Library

    2000-12-01

    STS098-S-002 (December 2000) --- These five astronauts have been in training for the STS-98 mission, scheduled for launch aboard the Space Shuttle Atlantis in January of 2001. The crew is composed of astronauts Kenneth D. Cockrell (right front), mission commander; and Mark L. Polansky (left front), pilot; along with astronauts Marsha S. Ivins, Robert L. Curbeam, Jr., (left rear) and Thomas D. Jones (right rear), all mission specialists. Curbeam and Jones are the scheduled extravehicular activity (EVA) participants for the International Space Station's 5a mission.

  15. Learning to rank image tags with limited training examples.

    PubMed

    Songhe Feng; Zheyun Feng; Rong Jin

    2015-04-01

    With an increasing number of images that are available in social media, image annotation has emerged as an important research topic due to its application in image matching and retrieval. Most studies cast image annotation into a multilabel classification problem. The main shortcoming of this approach is that it requires a large number of training images with clean and complete annotations in order to learn a reliable model for tag prediction. We address this limitation by developing a novel approach that combines the strength of tag ranking with the power of matrix recovery. Instead of having to make a binary decision for each tag, our approach ranks tags in the descending order of their relevance to the given image, significantly simplifying the problem. In addition, the proposed method aggregates the prediction models for different tags into a matrix, and casts tag ranking into a matrix recovery problem. It introduces the matrix trace norm to explicitly control the model complexity, so that a reliable prediction model can be learned for tag ranking even when the tag space is large and the number of training images is limited. Experiments on multiple well-known image data sets demonstrate the effectiveness of the proposed framework for tag ranking compared with the state-of-the-art approaches for image annotation and tag ranking.

  16. Molecular characterization of human ABHD2 as TAG lipase and ester hydrolase

    PubMed Central

    M., Naresh Kumar; V.B.S.C., Thunuguntla; G.K., Veeramachaneni; B., Chandra Sekhar; Guntupalli, Swapna; J.S., Bondili

    2016-01-01

    Alterations in lipid metabolism have been progressively documented as a characteristic property of cancer cells. Though, human ABHD2 gene was found to be highly expressed in breast and lung cancers, its biochemical functionality is yet uncharacterized. In the present study we report, human ABHD2 as triacylglycerol (TAG) lipase along with ester hydrolysing capacity. Sequence analysis of ABHD2 revealed the presence of conserved motifs G205XS207XG209 and H120XXXXD125. Phylogenetic analysis showed homology to known lipases, Drosophila melanogaster CG3488. To evaluate the biochemical role, recombinant ABHD2 was expressed in Saccharomyces cerevisiae using pYES2/CT vector and His-tag purified protein showed TAG lipase activity. Ester hydrolase activity was confirmed with pNP acetate, butyrate and palmitate substrates respectively. Further, the ABHD2 homology model was built and the modelled protein was analysed based on the RMSD and root mean square fluctuation (RMSF) of the 100 ns simulation trajectory. Docking the acetate, butyrate and palmitate ligands with the model confirmed covalent binding of ligands with the Ser207 of the GXSXG motif. The model was validated with a mutant ABHD2 developed with alanine in place of Ser207 and the docking studies revealed loss of interaction between selected ligands and the mutant protein active site. Based on the above results, human ABHD2 was identified as a novel TAG lipase and ester hydrolase. PMID:27247428

  17. Molecular characterization of human ABHD2 as TAG lipase and ester hydrolase.

    PubMed

    M, Naresh Kumar; V B S C, Thunuguntla; G K, Veeramachaneni; B, Chandra Sekhar; Guntupalli, Swapna; J S, Bondili

    2016-08-01

    Alterations in lipid metabolism have been progressively documented as a characteristic property of cancer cells. Though, human ABHD2 gene was found to be highly expressed in breast and lung cancers, its biochemical functionality is yet uncharacterized. In the present study we report, human ABHD2 as triacylglycerol (TAG) lipase along with ester hydrolysing capacity. Sequence analysis of ABHD2 revealed the presence of conserved motifs G(205)XS(207)XG(209) and H(120)XXXXD(125) Phylogenetic analysis showed homology to known lipases, Drosophila melanogaster CG3488. To evaluate the biochemical role, recombinant ABHD2 was expressed in Saccharomyces cerevisiae using pYES2/CT vector and His-tag purified protein showed TAG lipase activity. Ester hydrolase activity was confirmed with pNP acetate, butyrate and palmitate substrates respectively. Further, the ABHD2 homology model was built and the modelled protein was analysed based on the RMSD and root mean square fluctuation (RMSF) of the 100 ns simulation trajectory. Docking the acetate, butyrate and palmitate ligands with the model confirmed covalent binding of ligands with the Ser(207) of the GXSXG motif. The model was validated with a mutant ABHD2 developed with alanine in place of Ser(207) and the docking studies revealed loss of interaction between selected ligands and the mutant protein active site. Based on the above results, human ABHD2 was identified as a novel TAG lipase and ester hydrolase. © 2016 The Author(s).

  18. Neural net controlled tag gas sampling system for nuclear reactors

    DOEpatents

    Gross, Kenneth C.; Laug, Matthew T.; Lambert, John D. B.; Herzog, James P.

    1997-01-01

    A method and system for providing a tag gas identifier to a nuclear fuel rod and analyze escaped tag gas to identify a particular failed nuclear fuel rod. The method and system include disposing a unique tag gas composition into a plenum of a nuclear fuel rod, monitoring gamma ray activity, analyzing gamma ray signals to assess whether a nuclear fuel rod has failed and is emitting tag gas, activating a tag gas sampling and analysis system upon sensing tag gas emission from a failed nuclear rod and evaluating the escaped tag gas to identify the particular failed nuclear fuel rod.

  19. STS-31: APU Controller Removal

    NASA Technical Reports Server (NTRS)

    1990-01-01

    The launch April 10 of the STS-31 was scrubbed at T-4 minutes due to a faulty valve in auxiliary power unit (APU) number one. The auxiliary power unit is a hydrazine-fueled, turbine-driven power unit that generates mechanical shaft power to drive a hydraulic pump that produces pressure for the orbiter's hydraulic system. This video shows the removal of the STS-31's auxiliary power unit (APU).

  20. STS-32 Columbia, OV-102, liftoff from KSC LC Pad 39A is reflected in waterway

    NASA Image and Video Library

    1990-01-09

    STS032-S-069 (9 Jan. 1990) --- The space shuttle Columbia, with a five member crew aboard, lifts off for the ninth time as STS-32 begins a 10-day mission in Earth orbit. Leaving from Launch Pad 39A at 7:34:59:98 a.m. EST, in this horizontal (cropped 70mm) frame, Columbia is seen reflected in nearby marsh waters some 24 hours after dubious weather at the return-to-launch site (RTLS) had cancelled a scheduled launch. Onboard the spacecraft were astronauts Daniel C. Brandenstein, James D. Wetherbee, Bonnie J. Dunbar, G. David Low and Marsha S. Ivins. Photo credit: NASA

  1. STS-113 crew group photo during TCDT

    NASA Technical Reports Server (NTRS)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. -- The crews of Mission STS-113 gather for a group photograph on the 195-foot level of the Fixed Service Structure on Launch Pad 39A. From left are Expedition 6 cosmonaut Nikolai Budarin and astronaut Donald Pettit; STS-113 Pilot Paul Lockhart and Commander James Wetherbee; Expedition 6 Commander Ken Bowersox; STS-113 Mission Specialists Michael Lopez-Alegria and John Herrington. They have been participating in emergency egress training, part of Terminal Countdown Demonstration Test activities in preparation for their launch. The 16th assembly flight to the International Space Station, STS-113 will carry the Port 1 (P1) truss aboard Space Shuttle Endeavour, as well as Expedition 6, who will replace Expedition 5 on the Station. The mission is scheduled to launch Nov. 10, 2002.

  2. STS-113 crew group photo during TCDT

    NASA Technical Reports Server (NTRS)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. -- The crews of Mission STS-113 gather for a group photograph on the 195-foot level of the Fixed Service Structure on Launch Pad 39A. From left are STS-113 Pilot Paul Lockhart; Expedition 6 Commander Ken Bowersox; STS-113 Mission Specialists Michael Lopez-Alegria and John Herrington, and Commander James Wetherbee; Expedition 6 astronaut Donald Pettit and cosmonaut Nikolai Budarin. They have been participating in emergency egress training, part of Terminal Countdown Demonstration Test activities in preparation for their launch. The 16th assembly flight to the International Space Station, STS-113 will carry the Port 1 (P1) truss aboard Space Shuttle Endeavour, as well as Expedition 6, who will replace Expedition 5 on the Station. The mission is scheduled to launch Nov. 10, 2002.

  3. STS-131 Launch from Firing Room 4

    NASA Image and Video Library

    2010-04-05

    STS131-S-055 (5 April 2010) --- Assistant Launch Director Mike Leinbach (right) speaks with NASA commentator Mike Curie in Firing Room 4 in the Launch Control Center at NASA's Kennedy Space Center in Florida prior to the launch of space shuttle Discovery's STS-131 mission. The seven-member STS-131 crew will deliver the multi-purpose logistics module Leonardo, filled with supplies, a new crew sleeping quarters and science racks that will be transferred to the International Space Station's laboratories. The crew also will switch out a gyroscope on the station’s truss structure, install a spare ammonia storage tank and retrieve a Japanese experiment from the station’s exterior. STS-131 is the 33rd shuttle mission to the station and the 131st shuttle mission overall.

  4. 48 CFR 908.7101-7 - Government license tags.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Government license tags... ACQUISITION PLANNING REQUIRED SOURCES OF SUPPLIES AND SERVICES Acquisition of Special Items 908.7101-7 Government license tags. (a) Government license tags shall be procured and assignments recorded by DOE...

  5. STS-132 Space Shuttle Atlantis Launch

    NASA Image and Video Library

    2010-05-14

    STS132-S-015 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo Credit: NASA/Jack Pfaller

  6. STS-132 Space Shuttle Atlantis Launch

    NASA Image and Video Library

    2010-05-14

    STS132-S-016 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo Credit: NASA/Jack Pfaller

  7. STS-132 Space Shuttle Atlantis Launch

    NASA Image and Video Library

    2010-05-14

    STS132-S-017 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis. For more information on the STS-132 mission objectives, payload and crew, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts132/index.html. Photo Credit: NASA/Jack Pfaller

  8. STS-57 Endeavour, OV-105, framed by Florida vegetation, lifts off from KSC LC

    NASA Image and Video Library

    1993-06-21

    STS057-S-055 (21 June 1993) --- Framed by a variety of flora types, the Space Shuttle Endeavour lifts off Launch Pad 39B to begin the STS-57 mission. Launch occurred at 9:07:22 a.m. (EDT), June 21, 1993. The mission represents the first flight of the commercially developed SPACEHAB laboratory module and also will feature a retrieval of the European Retrievable Carrier (EURECA). Onboard for Endeavour's fourth flight are a crew of six - Ronald J. Grabe, mission commander; Brian Duffy, pilot; G. David Low, payload commander; and Nancy J. Sherlock, Peter J.K. (Jeff) Wisoff and Janice E. Voss, all mission specialists. An earlier launch attempt was scrubbed due to unacceptable weather conditions both at KSC and the overseas contingency landing sites.

  9. STS-32 crewmembers wave as they leave KSC O&C Bldg for launch pad

    NASA Image and Video Library

    1990-01-09

    STS032-S-056 (20 Jan 1990) --- STS-32 Columbia, Orbiter Vehicle (OV) 102, crewmembers depart the Kennedy Space Center (KSC) Operations and Checkout (O and C) Building enroute to KSC Launch Complex (LC) Pad 39A. Dubious weather at the return-to-launch site (RTLS) caused postponement of yesterday's planned launch. From left to right are Mission Specialist (MS) G. David Low, MS Marsha S. Ivins, MS Bonnie J. Dunbar, Pilot James D. Wetherbee, and Commander Daniel C. Brandenstein. All crewmembers are wearing launch and entry suits (LESs) and Low, Ivins, and Wetherbee wave to spectators as they head to the transportation van. Following the crew are astronaut Michael L. Coats (left) and NASA/JSC manager Donald R. Puddy.

  10. The STS-95 crew participates in a media briefing before returning to JSC

    NASA Technical Reports Server (NTRS)

    1998-01-01

    Members of the STS-95 crew file past photographers and reporters from all over the world as they enter the Kennedy Space Center Press Site Auditorium to participate in a media briefing before returning to the Johnson Space Center in Houston, Texas. From left to right, they are Mission Commander Curtis L. Brown Jr.; Pilot Steven W. Lindsey; Mission Specialist and Payload Commander Stephen K. Robinson; Mission Specialist Scott E. Parazynski; and Payload Specialist John H. Glenn Jr., a senator from Ohio and one of the original seven Project Mercury astronauts. The other STS- 95 crew members participating in the briefing (but hidden behind Parazynski) are Mission Specialist Pedro Duque, with the European Space Agency (ESA), and Payload Specialist Chiaki Mukai, with the National Space Development Agency of Japan (NASDA). The STS-95 mission ended with landing at Kennedy Space Center's Shuttle Landing Facility at 12:04 p.m. EST on Nov. 7. The mission included research payloads such as the Spartan-201 solar- observing deployable spacecraft, the Hubble Space Telescope Orbital Systems Test Platform, the International Extreme Ultraviolet Hitchhiker, as well as a SPACEHAB single module with experiments on space flight and the aging process.

  11. The STS-95 crew participates in a media briefing before returning to JSC

    NASA Technical Reports Server (NTRS)

    1998-01-01

    Members of the STS-95 crew file past photographers and reporters from all over the world as they enter the Kennedy Space Center Press Site Auditorium to participate in a media briefing before returning to the Johnson Space Center in Houston, Texas. From left to right, they are Mission Specialist Pedro Duque, with the European Space Agency (ESA); Payload Specialist Chiaki Mukai, with the National Space Development Agency of Japan (NASDA); and Payload Specialist John H. Glenn Jr., a senator from Ohio and one of the original seven Project Mercury astronauts. The other STS- 95 crew members participating in the briefing are Mission Commander Curtis L. Brown Jr., Pilot Steven W. Lindsey, Mission Specialist and Payload Commander Stephen K. Robinson, and Mission Specialist Scott E. Parazynski. The STS-95 mission ended with landing at Kennedy Space Center's Shuttle Landing Facility at 12:04 p.m. EST on Nov. 7. The mission included research payloads such as the Spartan-201 solar-observing deployable spacecraft, the Hubble Space Telescope Orbital Systems Test Platform, the International Extreme Ultraviolet Hitchhiker, as well as a SPACEHAB single module with experiments on space flight and the aging process.

  12. The STS-95 crew participates in a media briefing before returning to JSC

    NASA Technical Reports Server (NTRS)

    1998-01-01

    STS-95 Payload Specialist John H. Glenn Jr. (right), a senator from Ohio and one of the original seven Project Mercury astronauts, gestures during a media briefing at the Kennedy Space Center Press Site Auditorium. Glenn and the other members of the STS-95 crew held the briefing before returning to the Johnson Space Center in Houston, Texas. Others shown are (left to right) Mission Specialist Scott E. Parazynski; Mission Specialist Pedro Duque, with the European Space Agency (ESA); and Payload Specialist Chiaki Mukai, with the National Space Development Agency of Japan (NASDA). The other crew members participating in the briefing were Mission Commander Curtis L. Brown Jr., Pilot Steven W. Lindsey, and Mission Specialist and Payload Commander Stephen K. Robinson. The STS-95 mission ended with landing at Kennedy Space Center's Shuttle Landing Facility at 12:04 p.m. EST on Nov. 7. The mission included research payloads such as the Spartan-201 solar-observing deployable spacecraft, the Hubble Space Telescope Orbital Systems Test Platform, the International Extreme Ultraviolet Hitchhiker, as well as a SPACEHAB single module with experiments on space flight and the aging process.

  13. STS-84 Liftoff (across water)

    NASA Technical Reports Server (NTRS)

    1997-01-01

    The Space Shuttle Atlantis turns night into day for a few moments as it lifts off on May 15 at 4:07:48 a.m. EDT from Launch Pad 39A on the STS-84 mission. The fourth Shuttle mission of 1997 will be the sixth docking of the Space Shuttle with the Russian Space Station Mir. The commander is Charles J. Precourt. The pilot is Eileen Marie Collins. The five mission specialists are C. Michael Foale, Carlos I. Noriega, Edward Tsang Lu, Jean-Francois Clervoy of the European Space Agency and Elena V. Kondakova of the Russian Space Agency. The planned nine-day mission will include the exchange of Foale for U.S. astronaut and Mir 23 crew member Jerry M. Linenger, who has been on Mir since Jan. 15. Linenger transferred to Mir during the last docking mission, STS-81; he will return to Earth on Atlantis. Foale is slated to remain on Mir for about four months until he is replaced in September by STS-86 Mission Specialist Wendy B. Lawrence. During the five days Atlantis is scheduled to be docked with the Mir, the STS-84 crew and the Mir 23 crew, including two Russian cosmonauts, Commander Vasily Tsibliev and Flight Engineer Alexander Lazutkin, will participate in joint experiments. The STS-84 mission also will involve the transfer of more than 7,300 pounds of water, logistics and science equipment to and from the Mir. Atlantis is carrying a nearly 300-pound oxygen generator to replace one of two Mir units which have experienced malfunctions. The oxygen it generates is used for breathing by the Mir crew.

  14. STS-84 Shuttle Atlantis Liftoff

    NASA Technical Reports Server (NTRS)

    1997-01-01

    The Space Shuttle Atlantis turns night into day for a few moments as it lifts off on May 15 at 4:07:48 a.m. EDT from Launch Pad 39A on the STS-84 mission. The fourth Shuttle mission of 1997 will be the sixth docking of the Space Shuttle with the Russian Space Station Mir. The commander is Charles J. Precourt. The pilot is Eileen Marie Collins. The five mission specialists are C. Michael Foale, Carlos I. Noriega, Edward Tsang Lu, Jean-Francois Clervoy of the European Space Agency and Elena V. Kondakova of the Russian Space Agency. The planned nine-day mission will include the exchange of Foale for U.S. astronaut and Mir 23 crew member Jerry M. Linenger, who has been on Mir since Jan. 15. Linenger transferred to Mir during the last docking mission, STS-81; he will return to Earth on Atlantis. Foale is slated to remain on Mir for about four months until he is replaced in September by STS-86 Mission Specialist Wendy B. Lawrence. During the five days Atlantis is scheduled to be docked with the Mir, the STS-84 crew and the Mir 23 crew, including two Russian cosmonauts, Commander Vasily Tsibliev and Flight Engineer Alexander Lazutkin, will participate in joint experiments. The STS-84 mission also will involve the transfer of more than 7,300 pounds of water, logistics and science equipment to and from the Mir. Atlantis is carrying a nearly 300-pound oxygen generator to replace one of two Mir units which have experienced malfunctions. The oxygen it generates is used for breathing by the Mir crew.

  15. STS-87 crew participates in Crew Equipment Interface Test

    NASA Technical Reports Server (NTRS)

    1997-01-01

    Participating in the Crew Equipment Integration Test (CEIT) at Kennedy Space Center are STS-87 crew members Winston Scott, at left, and Takao Doi, Ph.D., of the National Space Development Agency of Japan, both mission specialists on STS-87. The CEIT gives astronauts an opportunity to get a hands-on look at the payloads with which they will be working on-orbit. STS-87 will be the fourth United States Microgravity Payload and flight of the Spartan-201 deployable satellite. During the STS-87 mission, scheduled for a Nov. 19 liftoff from KSC, Dr. Doi and Scott will both perform spacewalks. STS-87 is scheduled for a Nov. 19 liftoff from KSC.

  16. Individually Identifiable Surface Acoustic Wave Sensors, Tags and Systems

    NASA Technical Reports Server (NTRS)

    Hines, Jacqueline H. (Inventor); Solie, Leland P. (Inventor); Tucker, Dana Y. G. (Inventor); Hines, Andrew T. (Inventor)

    2017-01-01

    A surface-launched acoustic wave sensor tag system for remotely sensing and/or providing identification information using sets of surface acoustic wave (SAW) sensor tag devices is characterized by acoustic wave device embodiments that include coding and other diversity techniques to produce groups of sensors that interact minimally, reducing or alleviating code collision problems typical of prior art coded SAW sensors and tags, and specific device embodiments of said coded SAW sensor tags and systems. These sensor/tag devices operate in a system which consists of one or more uniquely identifiable sensor/tag devices and a wireless interrogator. The sensor device incorporates an antenna for receiving incident RF energy and re-radiating the tag identification information and the sensor measured parameter(s). Since there is no power source in or connected to the sensor, it is a passive sensor. The device is wirelessly interrogated by the interrogator.

  17. Ambitious STS-7 mission to feature first landing at Kennedy

    NASA Technical Reports Server (NTRS)

    Garrett, D.; Hess, M.; White, T.; Taylor, J.

    1982-01-01

    The STS-7 press briefing schedule, NASA select television schedule; launch preparations, countdown and liftoff; major countdown milestones; launch window; STS-7 flight sequence of events, landing timeline; STS-7 flight timeline; landing and post landing operations; flight objectives; Telesat's ANIK-C 2; PALAPA-B; STS-7 experiments; and spacecraft tracking and data network are presented.

  18. Testing archival tag technology in coho salmon

    USGS Publications Warehouse

    Nielsen, Jennifer L.; Richards, Philip; Tingey, Thor; Wilson, Derek; Zimmerman, Chris

    2004-01-01

    Archive tags with temperature and light-geolocation sensors will be monitored for post-smolt coho salmon in Cook Inlet. Light/location relationships specific to the Gulf of Alaska developed under Project 00478 will be applied in this study of movement and migration paths for coho salmon during maturation in ocean environments in Cook Inlet. Salmon for this study will be reared in captivity (at the Alaska Department of Fish and Game hatchery at Fort Richardson) to 1+ year of age (200-250mm) and released in Cook Inlet as part of the department's Ship Creek sport-fishing hatchery release. FY 01 includes pilot studies of tag retention, behavior, and growth for coho in captivity. Ship Creek coho will be tagged mid-May. A spring release experiment in the first year will be contingent on the successful implementation and retention of these tags. Surveys for early jack recoveries will be done at the Ship Creek weir and among sport fishers. Monitoring for adult tag recoveries will be done in the coho commercial fishery in Cook Inlet and the derby sport fishery on Ship Creek. Archive tagged fish will be used to document coho salmon use of marine habitats, migration routes, contribution to the sport fishery, and hatchery/wild interactions for salmon in Cook Inlet.

  19. STS-75 crew insignia

    NASA Image and Video Library

    1997-10-01

    STS075-S-001 (September 1995) --- The STS-75 crew patch depicts the space shuttle Columbia and the Tethered Satellite connected by a 21-kilometer electronically conducting tether. The orbiter/satellite system is passing through Earth?s magnetic field which, like an electronic generator, will produce thousands of volts of electricity. Columbia is carrying the United States Microgravity pallet to conduct microgravity research in material science and thermodynamics. The tether is crossing Earth?s terminator signifying the dawn of a new era for space tether applications and in mankind?s knowledge of Earth?s ionosphere, material science, and thermodynamics. The patch was designed for the STS-75 crew members by Mike Sanni. The NASA insignia design for space shuttle flights is reserved for use by the astronauts and for other official use as the NASA Administrator may authorize. Public availability has been approved only in the forms of illustrations by the various news media. When and if there is any change in this policy, which is not anticipated, the change will be publicly announced. Photo credit: NASA

  20. Neural net controlled tag gas sampling system for nuclear reactors

    DOEpatents

    Gross, K.C.; Laug, M.T.; Lambert, J.B.; Herzog, J.P.

    1997-02-11

    A method and system are disclosed for providing a tag gas identifier to a nuclear fuel rod and analyze escaped tag gas to identify a particular failed nuclear fuel rod. The method and system include disposing a unique tag gas composition into a plenum of a nuclear fuel rod, monitoring gamma ray activity, analyzing gamma ray signals to assess whether a nuclear fuel rod has failed and is emitting tag gas, activating a tag gas sampling and analysis system upon sensing tag gas emission from a failed nuclear rod and evaluating the escaped tag gas to identify the particular failed nuclear fuel rod. 12 figs.

  1. Polyvalent Display of Heme on Hepatitis B Virus Capsid Protein through Coordination to Hexahistidine Tags

    PubMed Central

    Prasuhn, Duane E.; Kuzelka, Jane; Strable, Erica; Udit, Andrew K.; Cho, So-Hye; Lander, Gabriel C.; Quispe, Joel D.; Diers, James R.; Bocian, David F.; Potter, Clint; Carragher, Bridget; Finn, M.G.

    2009-01-01

    SUMMARY The addition of a hexahistidine tag to the N terminus of the hepatitis B capsid protein gives rise to a self-assembled particle with 80 sites of high local density of histidine side chains. Iron protoporphyrin IX has been found to bind tightly at each of these sites, making a polyvalent system of well-defined spacing between metalloporphyrin complexes. The spectroscopic and redox properties of the resulting particle are consistent with the presence of 80 site-isolated bis(histidine)-bound heme centers, comprising a polyvalent b-type cytochrome mimic. PMID:18482703

  2. STS-41G earth observations

    NASA Image and Video Library

    1984-10-08

    41G-38-060 (5-13 Oct 1984) --- A large ship wake in the central Mediterranean Sea is highlighted by sunglint in this handheld camera's view from STS-41G. According to NASA and U.S. Navy scientists studying the STS-41G film, the depicted reflectance is similar to oil slicks seen in the various oceans of the world. They note that it is apparent that a ship is pumping a mixture of bilge water and oil overboard. The crew consisted of astronauts Robert L. Crippen, commander; Jon A. McBride, pilot; mission specialist's Kathryn D. Sullivan, Sally K. Ride, and David D. Leestma; Canadian astronaut Marc Garneau; and Paul D. Scully-Power, payload specialist. EDITOR'S NOTE: The STS-41G mission had the first American female EVA (Sullivan); first seven-person crew; first orbital fuel transfer; and the first Canadian (Garneau).

  3. Myocardial motion estimation of tagged cardiac magnetic resonance images using tag motion constraints and multi-level b-splines interpolation.

    PubMed

    Liu, Hong; Yan, Meng; Song, Enmin; Wang, Jie; Wang, Qian; Jin, Renchao; Jin, Lianghai; Hung, Chih-Cheng

    2016-05-01

    Myocardial motion estimation of tagged cardiac magnetic resonance (TCMR) images is of great significance in clinical diagnosis and the treatment of heart disease. Currently, the harmonic phase analysis method (HARP) and the local sine-wave modeling method (SinMod) have been proven as two state-of-the-art motion estimation methods for TCMR images, since they can directly obtain the inter-frame motion displacement vector field (MDVF) with high accuracy and fast speed. By comparison, SinMod has better performance over HARP in terms of displacement detection, noise and artifacts reduction. However, the SinMod method has some drawbacks: 1) it is unable to estimate local displacements larger than half of the tag spacing; 2) it has observable errors in tracking of tag motion; and 3) the estimated MDVF usually has large local errors. To overcome these problems, we present a novel motion estimation method in this study. The proposed method tracks the motion of tags and then estimates the dense MDVF by using the interpolation. In this new method, a parameter estimation procedure for global motion is applied to match tag intersections between different frames, ensuring specific kinds of large displacements being correctly estimated. In addition, a strategy of tag motion constraints is applied to eliminate most of errors produced by inter-frame tracking of tags and the multi-level b-splines approximation algorithm is utilized, so as to enhance the local continuity and accuracy of the final MDVF. In the estimation of the motion displacement, our proposed method can obtain a more accurate MDVF compared with the SinMod method and our method can overcome the drawbacks of the SinMod method. However, the motion estimation accuracy of our method depends on the accuracy of tag lines detection and our method has a higher time complexity. Copyright © 2015 Elsevier Inc. All rights reserved.

  4. 29 CFR 1915.89 - Control of hazardous energy (lockout/tags-plus).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... employee: (A) Sign a group tag (or a group tag equivalent), attach a personal identification device to a... group tag (or the group tag equivalent), remove the personal identification device, or perform a... safe exposure status of each authorized employee, and (b) signs a group tag (or a group tag equivalent...

  5. 29 CFR 1915.89 - Control of hazardous energy (lockout/tags-plus).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... employee: (A) Sign a group tag (or a group tag equivalent), attach a personal identification device to a... group tag (or the group tag equivalent), remove the personal identification device, or perform a... safe exposure status of each authorized employee, and (b) signs a group tag (or a group tag equivalent...

  6. 29 CFR 1915.89 - Control of hazardous energy (lockout/tags-plus).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... employee: (A) Sign a group tag (or a group tag equivalent), attach a personal identification device to a... group tag (or the group tag equivalent), remove the personal identification device, or perform a... safe exposure status of each authorized employee, and (b) signs a group tag (or a group tag equivalent...

  7. Spawning site fidelity and apparent annual survival of walleye (Sander vitreus) differ between a Lake Huron and Lake Erie tributary

    USGS Publications Warehouse

    Hayden, Todd A.; Binder, Thomas; Holbrook, Christopher; Vandergoot, Christopher; Fielder, David G.; Cooke, Steven J.; Dettmers, John M.; Krueger, Charles C.

    2018-01-01

    Fidelity to spawning habitats can maximise reproductive success of fish by synchronising movements to sites of previous recruitment. To determine the role of reproductive fidelity in structuring walleye Sander vitreus populations in the Laurentian Great Lakes, we used acoustic telemetry combined with Cormack–Jolly–Seber capture–recapture models to estimate spawning site fidelity and apparent annual survival for the Tittabawassee River in Lake Huron and Maumee River in Lake Erie. Walleye in spawning condition were tagged from the Tittabawassee River in Lake Huron and Maumee River in Lake Erie in 2011–2012. Site fidelity and apparent annual survival were estimated from return of individuals to the stream where tagged. Site fidelity estimates were higher in the Tittabawassee River (95%) than the Maumee River (70%) and were not related to sex or fish length at tagging. Apparent annual survival of walleye tagged in the Tittabawassee did not differ among spawning seasons but was higher for female than male walleye and decreased linearly as fish length increased. Apparent annual survival of walleye tagged in the Maumee River did not differ among spawning seasons but was higher for female walleye than male walleye and increased linearly as fish length increased. Greater fidelity of walleye tagged in the Tittabawassee River than walleye tagged in the Maumee River may be related to the close proximity to the Maumee River of other spawning aggregations and multiple spawning sites in Lake Erie. As spawning site fidelity increases, management actions to conserve population structure require an increasing focus on individual stocks.

  8. Comparison of Luminex NxTAG Respiratory Pathogen Panel and xTAG Respiratory Viral Panel FAST Version 2 for the Detection of Respiratory Viruses

    PubMed Central

    Lee, Chun Kiat; Lee, Hong Kai; Ng, Christopher Wei Siong; Chiu, Lily; Tang, Julian Wei-Tze; Loh, Tze Ping

    2017-01-01

    Owing to advancements in molecular diagnostics, recent years have seen an increasing number of laboratories adopting respiratory viral panels to detect respiratory pathogens. In December 2015, the NxTAG respiratory pathogen panel (NxTAG RPP) was approved by the United States Food and Drug Administration. We compared the clinical performance of this new assay with that of the xTAG respiratory viral panel (xTAG RVP) FAST v2 using 142 clinical samples and 12 external quality assessment samples. Discordant results were resolved by using a laboratory-developed respiratory viral panel. The NxTAG RPP achieved 100% concordant negative results and 86.6% concordant positive results. It detected one coronavirus 229E and eight influenza A/H3N2 viruses that were missed by the xTAG RVP FAST v2. On the other hand, the NxTAG RPP missed one enterovirus/rhinovirus and one metapneumovirus that were detected by FAST v2. Both panels correctly identified all the pathogens in the 12 external quality assessment samples. Overall, the NxTAG RPP demonstrated good diagnostic performance. Of note, it was better able to subtype the influenza A/H3N2 viruses compared with the xTAG RVP FAST v2. PMID:28224774

  9. STS-69 preflight crew portrait

    NASA Image and Video Library

    1995-06-01

    STS069-S-002 (June 1995) --- These five NASA astronauts have been named as crewmembers for the STS-69 mission, scheduled onboard the Space Shuttle Endeavour in late July of 1995. David M. Walker (right front) is mission commander; with Kenneth D. Cockrell (left front) scheduled to serve as pilot. On the back row are (left to right) Michael L. Gernhardt and James H. Newman, both mission specialists; and James S. Voss, payload commander.

  10. 50 CFR 635.33 - Archival tags.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 12 2013-10-01 2013-10-01 false Archival tags. 635.33 Section 635.33 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE ATLANTIC HIGHLY MIGRATORY SPECIES Management Measures § 635.33 Archival tags. (a...

  11. 50 CFR 20.81 - Tagging requirement.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... PLANTS (CONTINUED) MIGRATORY BIRD HUNTING Migratory Bird Preservation Facilities § 20.81 Tagging requirement. No migratory bird preservation facility shall receive or have in custody any migratory game birds unless such birds are tagged as required by § 20.36. [41 FR 31537, July 29, 1976] ...

  12. 50 CFR 20.81 - Tagging requirement.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... PLANTS (CONTINUED) MIGRATORY BIRD HUNTING Migratory Bird Preservation Facilities § 20.81 Tagging requirement. No migratory bird preservation facility shall receive or have in custody any migratory game birds unless such birds are tagged as required by § 20.36. [41 FR 31537, July 29, 1976] ...

  13. Serotype determination of Salmonella by xTAG assay.

    PubMed

    Zheng, Zhibei; Zheng, Wei; Wang, Haoqiu; Pan, Jincao; Pu, Xiaoying

    2017-10-01

    Currently, no protocols or commercial kits are available to determine the serotypes of Salmonella by using Luminex MAGPIX®. In this study, an xTAG assay for serotype determination of Salmonella suitable for Luminex MAGPIX® is described and 228 Salmonella isolates were serotype determined by this xTAG assay. The xTAG assay consists of two steps: 1) Multiplex PCR to amplify simultaneously O, H and Vi antigen genes of Salmonella, and 2) Magplex-TAG™ microsphere hybridization to identify accurately the specific PCR products of different antigens. Compared with the serotyping results of traditional serum agglutination test, the sensitivity and specificity of the xTAG assay were 95.1% and 100%, respectively. The agreement rate of these two assays was 95.2%. Compared with Luminex xMAP® Salmonella Serotyping Assay (SSA) kit, the advantages of this xTAG assay are: First, the magnetic beads make it applicable to both the Luminex®100/200™ and MAGPIX® systems. Second, only primers rather than both primers and probes are needed in the xTAG assay, and the process of coupling antigen-specific oligonucleotide probes to beads is circumvented, which make the xTAG assay convenient to be utilized by other laboratories. The xTAG assay may serve as a rapid alternative or complementary method for traditional Salmonella serotyping tests, especially for laboratories that utilize the MAGPIX® systems. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. STS-112 Launch

    NASA Technical Reports Server (NTRS)

    2002-01-01

    Space Shuttle Orbiter Atlantis hurdles toward space from Launch Pad 39B at Kennedy Space Center in Florida for the STS-112 mission. Liftoff occurred at 3:46pm EDT, October 7, 2002. Atlantis carried the Starboard-1 (S1) Integrated Truss Structure and the Crew and Equipment Translation Aid (CETA) Cart A. The S1 was the second truss structure installed on the International Space Station (ISS). It was attached to the S0 truss which was previously installed by the STS-110 mission. The CETA is the first of two human-powered carts that ride along the ISS railway, providing mobile work platforms for future space walking astronauts. The 11 day mission performed three space walks to attach the S1 truss.

  15. STS116-S-001

    NASA Image and Video Library

    2006-07-01

    STS116-S-001 (July 2006) --- The STS-116 patch design signifies the continuing assembly of the International Space Station (ISS). The primary mission objective is to deliver and install the P5 truss element. The P5 installation will be conducted during the first of three planned spacewalks, and will involve use of both the shuttle and station robotic arms. The remainder of the mission will include a major reconfiguration and activation of the ISS electrical and thermal control systems, as well as delivery of Zvezda Service Module debris panels, which will increase ISS protection from potential impacts of micro-meteorites and orbital debris. In addition, a single expedition crewmember will launch on STS-116 to remain onboard the station, replacing an expedition crew member that will fly home with the shuttle crew. The crew patch depicts the space shuttle rising above the Earth and ISS. The United States and Swedish flags trail the orbiter, depicting the international composition of the STS-116 crew. The seven stars of the constellation Ursa Major are used to provide direction to the North Star, which is superimposed over the installation location of the P5 truss on ISS. The NASA insignia design for space shuttle flights is reserved for use by the astronauts and for other official use as the NASA Administrator may authorize. Public availability has been approved only in the forms of illustrations by the various news media. When and if there is any change in this policy, which is not anticipated, the change will be publicly announced. Photo credit: NASA

  16. Unity and Diversity in STS Curricula.

    ERIC Educational Resources Information Center

    de la Mothe, John R.

    The study of science, technology, and society (STS) has, over the past decade, become established in Great Britain, Canada, and the United States as the fastest growing area of teaching and research in institutes of higher education. While it is reasonable to describe STS as an important socio-educational movement, it is thematically erroneous to…

  17. Post flight press conference for the STS-7 mission

    NASA Technical Reports Server (NTRS)

    1983-01-01

    Two of the three mission specialists for STS-7 field questions from the press during the post-flight press conference in JSC's main auditorium on July 1, 1983. Left to right are John M. Fabian and Dr. Norman E. Thagard (35419); Portrait view of Fabian during the STS-7 post-flight press conference (35420); Portrait view of mission specialist Dr. Sally K. Ride during the STS-7 post-flight press conference (35421); Portrait view of STS-7 pilot Frederick H. Hauck during the post-flight press conference (35422); Portrait view of STS-7 crew commander Robert L. Crippen during the post-flight press conference (35423); Three STS-7 crew members listen to questions from news reporters. They are, left to right, Crippen, Hauck, and Ride (35424); The first five person shuttle crew and first woman crew member greet the news media. Members are, left to right, Crippen, Hauck, Ride, Fabian and Thagard (35425).

  18. Space Shuttle Atlantis Landing / STS-129 Mission

    NASA Image and Video Library

    2009-11-27

    PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - Space shuttle Atlantis touches down on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

  19. Effects of coded-wire-tagging on stream-dwelling Sea Lamprey larvae

    USGS Publications Warehouse

    Johnson, Nicholas; Swink, William D.; Dawson, Heather A.; Jones, Michael L.

    2016-01-01

    The effects of coded wire tagging Sea Lamprey Petromyzon marinus larvae from a known-aged stream-dwelling population were assessed. Tagged larvae were significantly shorter on average than untagged larvae from 3 to 18 months after tagging. However, 30 months after tagging, the length distribution of tagged and untagged larvae did not differ and tagged Sea Lampreys were in better condition (i.e., higher condition factor) and more likely to have undergone metamorphosis than the untagged population. The reason why tagged larvae were more likely to metamorphose is not clear, but the increased likelihood of metamorphosis could have been a compensatory response to the period of slower growth after tagging. Slower growth after tagging was consistent across larval size-classes, so handling and displacement from quality habitat during the early part of the growing season was likely the cause rather than the tag burden. The tag effects observed in this study, if caused by displacement and handling, may be minimized in future studies if tagging is conducted during autumn after growth has concluded for the year.

  20. STS-93 CEIT tests in OPF 3

    NASA Technical Reports Server (NTRS)

    1998-01-01

    In the Orbiter Processing Facility Bay 3, during the Crew Equipment Interface Test (CEIT), Mission Specialist Catherine G. Coleman (left) and Mission Commander Eileen M. Collins (right) check equipment that will fly on mission STS-93. The STS-93 mission will deploy the Advanced X-ray Astrophysics Facility (AXAF) which comprises three major elements: the spacecraft, the telescope, and the science instrument module (SIM). AXAF will allow scientists from around the world to obtain unprecedented X-ray images of a variety of high-energy objects to help understand the structure and evolution of the universe. Collins is the first woman to serve as a shuttle mission commander. The other STS-93 crew members are Pilot Jeffrey S. Ashby, Mission Specialist Steven A. Hawley and Mission Specialist Michel Tognini of France. Targeted date for the launch of STS-93 is March 18, 1999

  1. TagDigger: user-friendly extraction of read counts from GBS and RAD-seq data.

    PubMed

    Clark, Lindsay V; Sacks, Erik J

    2016-01-01

    In genotyping-by-sequencing (GBS) and restriction site-associated DNA sequencing (RAD-seq), read depth is important for assessing the quality of genotype calls and estimating allele dosage in polyploids. However, existing pipelines for GBS and RAD-seq do not provide read counts in formats that are both accurate and easy to access. Additionally, although existing pipelines allow previously-mined SNPs to be genotyped on new samples, they do not allow the user to manually specify a subset of loci to examine. Pipelines that do not use a reference genome assign arbitrary names to SNPs, making meta-analysis across projects difficult. We created the software TagDigger, which includes three programs for analyzing GBS and RAD-seq data. The first script, tagdigger_interactive.py, rapidly extracts read counts and genotypes from FASTQ files using user-supplied sets of barcodes and tags. Input and output is in CSV format so that it can be opened by spreadsheet software. Tag sequences can also be imported from the Stacks, TASSEL-GBSv2, TASSEL-UNEAK, or pyRAD pipelines, and a separate file can be imported listing the names of markers to retain. A second script, tag_manager.py, consolidates marker names and sequences across multiple projects. A third script, barcode_splitter.py, assists with preparing FASTQ data for deposit in a public archive by splitting FASTQ files by barcode and generating MD5 checksums for the resulting files. TagDigger is open-source and freely available software written in Python 3. It uses a scalable, rapid search algorithm that can process over 100 million FASTQ reads per hour. TagDigger will run on a laptop with any operating system, does not consume hard drive space with intermediate files, and does not require programming skill to use.

  2. 9 CFR 2.51 - Form of official tag.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Form of official tag. 2.51 Section 2.51 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.51 Form of official tag. (a) The official tag...

  3. The Effects of Target Audience on Social Tagging

    ERIC Educational Resources Information Center

    Alsarhan, Hesham

    2013-01-01

    Online social bookmarking systems allow users to assign tags (i.e., keywords) to represent the content of resources. Research on the effects of target audience on social tagging suggests that taggers select different tags for themselves, their community (e.g., family, friends, colleagues), and the general public (Panke & Gaiser, 2009; Pu &…

  4. 9 CFR 2.51 - Form of official tag.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Form of official tag. 2.51 Section 2.51 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.51 Form of official tag. (a) The official tag...

  5. 9 CFR 2.51 - Form of official tag.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Form of official tag. 2.51 Section 2.51 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.51 Form of official tag. (a) The official tag...

  6. 9 CFR 2.51 - Form of official tag.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Form of official tag. 2.51 Section 2.51 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.51 Form of official tag. (a) The official tag...

  7. 9 CFR 2.51 - Form of official tag.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Form of official tag. 2.51 Section 2.51 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.51 Form of official tag. (a) The official tag...

  8. STS-84 Crew Portrait

    NASA Technical Reports Server (NTRS)

    1997-01-01

    The crew assigned to the STS-84 mission included (seated front left to right) Jerry M Linenger, mission specialist; Charles J. Precourt, commander; and C. Michael Foale, mission specialist. On the back row (left to right) are Jean-Francois Clervoy (ESA), mission specialist; Eileen M. Collins, pilot; Edward T. Lu, mission specialist; Elena V. Kondakova (RSA), mission specialist; and Carlos I. Noriega, mission specialist. Launched aboard the Space Shuttle Atlantis on May 15, 1997 at 4:07:48 am (EDT), the STS-84 mission served as the sixth U.S. Space Shuttle-Russian Space Station Mir docking.

  9. STS-86 Crew Portrait

    NASA Technical Reports Server (NTRS)

    1997-01-01

    The crew assigned to the STS-86 mission included five U.S. astronauts, one Russian cosmonaut, and one Canadian astronaut. Kneeling is mission specialist Scott E. Parazynski. Others, pictured from left to right, are Michael J. Bloomfield, pilot; David A. Wolf, mission specialist; James D. Wetherbee, commander; and mission specialists Wendy B. Lawrence, Vlamimir G. Titov (RSA), and Jean-Loup J.M. Chretien (CNES). Launched aboard the Space Shuttle Atlantis on September 25, 1997 at 10:34:19 pm (EDT), the STS-86 mission served as the 7th U.S. Space Shuttle-Russian Space Station Mir docking.

  10. STS-88 Crew Portrait

    NASA Technical Reports Server (NTRS)

    1998-01-01

    Five NASA astronauts and a Russian cosmonaut assigned to the STS-88 mission pose for a crew portrait. Seated in front (left to right) are mission specialists Sergei K. Krikalev, representing the Russian Space Agency (RSA), and astronaut Nancy J. Currie. In the rear from the left, are astronauts Jerry L. Ross, mission specialist; Robert D. Cabana, mission commander; Frederick W. 'Rick' Sturckow, pilot; and James H. Newman, mission specialist. The STS-88 mission launched aboard the Space Shuttle Endeavor on December 4, 1998 at 2:35 a.m. (CST) to deliver the Unity Node to the International Space Station (ISS).

  11. STS-101 Crew Portrait

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Six astronauts and a Russian cosmonaut comprised the STS-101 mission that launched aboard the Space Shuttle Atlantis on May 19, 2000 at 5:11 am (CDT). Seated in front are astronauts James D. Halsell (right), mission commander; and Scott J. Horowitz, pilot. Others, from the left, are Mary Ellen Weber, Jeffrey N. Williams, Yury V. Usachev, James S. Voss and Susan J. Helms, all mission specialists. Usachev represents the Russian Space Agency (RSA). The crew of the STS- 101 mission refurbished and replaced components in both the Zarya and Unity modules, with top priority being the Zarya module.

  12. STS-63 Crew Portrait

    NASA Technical Reports Server (NTRS)

    1994-01-01

    Crew members assigned to the STS-63 mission included (front left to right) Janice E. Voss, mission specialist; Eileen M. Collins, pilot; (the first woman to pilot a Space Shuttle), James D. Wetherbee, commander; and Vladmir G. Titov (Cosmonaut). Standing in the rear are mission specialists Bernard A. Harris, and C. Michael Foale. Launched aboard the Space Shuttle Discovery on February 3, 1995 at 12:22:04 am (EST), the primary payload for the mission was the SPACEHAB-3. STS-63 marked the first approach and fly around by the Shuttle with the Russian space station Mir.

  13. STS-122 crew portrait

    NASA Image and Video Library

    2006-07-21

    STS122-S-002 (24 April 2007) --- These seven astronauts take a break from training to pose for the STS-122 crew portrait. From the left (front row) are astronauts Stephen N. Frick, commander; European Space Agency's (ESA) Leopold Eyharts; and Alan G. Poindexter, pilot. From the left (back row) are astronauts Leland D. Melvin, Rex J. Walheim, Stanley G. Love and European Space Agency's (ESA) Hans Schlegel, all mission specialists. Eyharts will join Expedition 16 in progress to serve as a flight engineer aboard the International Space Station. The crewmembers are attired in training versions of their shuttle launch and entry suits.

  14. sts093-s-009

    NASA Image and Video Library

    2009-09-23

    STS93-S-009 (23 July 1999) --- The Space Shuttle Columbia lifts off from Launch Pad 39B to begin the five-day STS-93 mission. After two unsuccessful attempts earlier in the week, liftoff occurred at 12:31 a.m. (EDT), July 23, 1999. Only hours after this picture was taken, the five-member crew released the Chandra X-Ray Observatory into orbit. Onboard were astronauts Eileen M. Collins, first woman shuttle mission commander; Jeffrey S. Ashby, pilot; and Steven A. Hawley, Catherine G. Coleman and Michel Tognini, all mission specialists. Tognini represents the Centre National d'Etudes Spatiales (CNES) of France.

  15. Anti-collision radio-frequency identification system using passive SAW tags

    NASA Astrophysics Data System (ADS)

    Sorokin, A. V.; Shepeta, A. P.

    2017-06-01

    Modern multi sensor systems should have high operating speed and resistance to climate impacts. Radiofrequency systems use passive SAW tags for identification items and vehicles. These tags find application in industry, traffic remote control systems, and railway remote traffic control systems for identification and speed measuring. However, collision of the passive SAW RFID tags hinders development passive RFID SAW technology in Industry. The collision problem for passive SAW tags leads for incorrect identification and encoding each tag. In our researching, we suggest approach for identification of several passive SAW tags in collision case.

  16. Current test results for the Athena radar responsive tag

    NASA Astrophysics Data System (ADS)

    Ormesher, Richard C.; Martinez, Ana; Plummer, Kenneth W.; Erlandson, David; Delaware, Sheri; Clark, David R.

    2006-05-01

    Sandia National Laboratories has teamed with General Atomics and Sierra Monolithics to develop the Athena tag for the Army's Radar Tag Engagement (RaTE) program. The radar-responsive Athena tag can be used for Blue Force tracking and Combat Identification (CID) as well as data collection, identification, and geolocation applications. The Athena tag is small (~4.5" x 2.4" x 4.2"), battery-powered, and has an integral antenna. Once remotely activated by a Synthetic Aperture Radar (SAR) or Moving Target Indicator (MTI) radar, the tag transponds modulated pulses to the radar at a low transmit power. The Athena tag can operate Ku-band and X-band airborne SAR and MTI radars. This paper presents results from current tag development testing activities. Topics covered include recent field tests results from the AN/APY-8 Lynx, F16/APG-66, and F15E/APG-63 V(1) radars and other Fire Control radars. Results show that the Athena tag successfully works with multiple radar platforms, in multiple radar modes, and for multiple applications. Radar-responsive tags such as Athena have numerous applications in military and government arenas. Military applications include battlefield situational awareness, combat identification, targeting, personnel recovery, and unattended ground sensors. Government applications exist in nonproliferation, counter-drug, search-and-rescue, and land-mapping activities.

  17. Retention of internal anchor tags by juvenile striped bass

    USGS Publications Warehouse

    Van Den Avyle, M.J.; Wallin, J.E.

    2001-01-01

    We marked hatchery-reared striped bass Morone saxatilis (145-265 mm total length) with internal anchor tags and monitored retention for 28 months after stocking in the Savannah River, Georgia and South Carolina. Anchor tags (with an 18-mm, T-shaped anchor and 42-mm streamer) were surgically implanted ventrally, and coded wire tags (1 mm long and 0.25 mm in diameter) were placed into the cheek muscle to help identify subsequent recaptures. The estimated probability of retention (SD) of anchor tags was 0.94 (0.05) at 4 months, 0.64 (0.13) at 16 months, and 0.33 (0.19) at 28 months. Of 10 fish recaptured with only coded wire tags, 5 showed an externally visible wound or scar near the point of anchor tag insertion. The incidence of wounds or scars, which we interpreted as evidence of tag shedding, increased to 50% in recaptures taken at 28 months (three of six fish). Our estimates for retention of anchor tags were generally lower than those in other studies of striped bass, possibly because of differences in the style of anchor or sizes of fish used. Because of its low rate of retention, the type of anchor tag we used may not be suitable for long-term assessments of stock enhancement programs that use striped bass of the sizes we evaluated.

  18. A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli

    PubMed Central

    2012-01-01

    Background The discovery of the autotransporter family has provided a mechanism for surface expression of proteins in laboratory strains of Escherichia coli. We have previously reported the use of the AIDA-I autotransport system to express the Salmonella enterica serovar Enteritidis proteins SefA and H:gm. The SefA protein was successfully exposed to the medium, but the orientation of H:gm in the outer membrane could not be determined due to proteolytic cleavage of the N-terminal detection-tag. The goal of the present work was therefore to construct a vector containing elements that facilitates analysis of surface expression, especially for proteins that are sensitive to proteolysis or otherwise difficult to express. Results The surface expression system pAIDA1 was created with two detection tags flanking the passenger protein. Successful expression of SefA and H:gm on the surface of E. coli was confirmed with fluorescently labeled antibodies specific for the N-terminal His6-tag and the C-terminal Myc-tag. While both tags were detected during SefA expression, only the Myc-tag could be detected for H:gm. The negative signal indicates a proteolytic cleavage of this protein that removes the His6-tag facing the medium. Conclusions Expression levels from pAIDA1 were comparable to or higher than those achieved with the formerly used vector. The presence of the Myc- but not of the His6-tag on the cell surface during H:gm expression allowed us to confirm the hypothesis that this fusion protein was present on the surface and oriented towards the cell exterior. Western blot analysis revealed degradation products of the same molecular weight for SefA and H:gm. The size of these fragments suggests that both fusion proteins have been cleaved at a specific site close to the C-terminal end of the passenger. This proteolysis was concluded to take place either in the outer membrane or in the periplasm. Since H:gm was cleaved to a much greater extent then the three times smaller Sef

  19. STS-125 Crew Visit Italian Embassy

    NASA Image and Video Library

    2009-07-22

    Members of the crew of STS-125, from left, Mission Specialists Andrew Feustel, Michael Good, Commander Scott Altman and Mission Specialist John Grunsfeld share a laugh with about the STS-125 mission to the Hubble Space Telescope during a visit by the crew to the Embassy of Italy in Washington, Thursday, July 23, 2009. Photo Credit: (NASA/Bill Ingalls)

  20. STS-98 on-orbit crew portrait

    NASA Image and Video Library

    2001-02-07

    STS098-365-0034 (7-20 February 2001) --- The crew of the STS-98 mission poses for the traditional inflight portrait on the flight deck of the Space Shuttle Atlantis. From left are astronauts Kenneth D. Cockrell, mission commander; Marsha S. Ivins, mission specialist; Thomas D. Jones, mission specialist; Mark L. Polansky, pilot; and Robert L. Curbeam, mission specialist.

  1. Radio-tracking manatees from land and space: tag design, implementation, and lessons learned from long-term study

    USGS Publications Warehouse

    Deutsch, C.J.; Bonde, R.K.; Reid, J.P.

    1998-01-01

    West Indian manatees (Trichechus manatus) were tracked along the Atlantic coast of Florida and Georgia (N = 83 manatees, n = 439 tag deployments, 1986-1996) and in eastern Puerto Rico (N = 8, n = 43, 1992-1996) using conventional and satellite-based radio-telemetry systems. A floating radio-tag, attached by a flexible tether to a padded belt around the base of the tail, enabled us to track manatees in saltwater environments. The tag incorporated VHF (very high frequency) and ultrasonic transmitters for field tracking and tag recovery, and an Argos satellite-monitored transmitter for remote tracking. We located each animal in the field about twice per week, received more than 60 000 good-quality Argos locations, and recovered tags in over 90% of deployments. The tag was designed to detach from the belt when entangled to prevent injury or drowning, and this often led to premature termination of tracking bouts. We had considerable success, however, in retagging belted manatees without recapture (97% of 392 retagging events). Most individuals were radio-tagged more than once (median = 3.0, maximum = 43) for a median total duration of 7.5 months (maximum = 6.8 yr). Data obtained through Argos have been valuable in addressing questions relating to long-distance movements, site fidelity, and identification of high-use areas. Fine-scale analyses of manatee habitat use and movements may require restricting the data set to the highest location quality or developing new analytical techniques to incorporate locational error. Field tracking provided useful ancillary data on life-history parameters, but sample sizes were small and survival estimates imprecise. Modification of the existing tag design to include Global Positioning System (GPS) functionality, with its finer spatial and temporal resolution, will offer new opportunities to address critical research and management problems facing this endangered species.

  2. Glycoproteomics enabled by tagging sialic acid- or galactose-terminated glycans

    PubMed Central

    Ramya, T N C; Weerapana, Eranthie; Cravatt, Benjamin F; Paulson, James C

    2013-01-01

    In this paper, we present two complementary strategies for enrichment of glycoproteins on living cells that combine the desirable attributes of “robust enrichment” afforded by covalent-labeling techniques and “specificity for glycoproteins” typically provided by lectin or antibody affinity reagents. Our strategy involves the selective introduction of aldehydes either into sialic acids by periodate oxidation (periodate oxidation and aniline-catalyzed oxime ligation (PAL)) or into terminal galactose and N-acetylgalactosamine residues by galactose oxidase (galactose oxidase and aniline-catalyzed oxime ligation (GAL)), followed by aniline-catalyzed oxime ligation with aminooxy-biotin to biotinylate the glycans of glycoprotein subpopulations with high efficiency and cell viability. As expected, the two methods exhibit reciprocal tagging efficiencies when applied to fully sialylated cells compared with sialic acid-deficient cells. To assess the utility of these labeling methods for glycoproteomics, we enriched the PAL- and GAL-labeled (biotinylated) glycoproteome by adsorption onto immobilized streptavidin. Glycoprotein identities (IDs) and N-glycosylation site information were then obtained by liquid chromatography-tandem mass spectrometry on total tryptic peptides and on peptides subsequently released from N-glycans still bound to the beads using peptide N-glycosidase F. A total of 175 unique N-glycosylation sites were identified, belonging to 108 nonredundant glycoproteins. Of the 108 glycoproteins, 48 were identified by both methods of labeling and the remainder was identified using PAL on sialylated cells (40) or GAL on sialic acid-deficient cells (20). Our results demonstrate that PAL and GAL can be employed as complementary methods of chemical tagging for targeted proteomics of glycoprotein subpopulations and identification of glycosylation sites of proteins on cells with an altered sialylation status. PMID:23070960

  3. Tagging Water Sources in Atmospheric Models

    NASA Technical Reports Server (NTRS)

    Bosilovich, M.

    2003-01-01

    Tagging of water sources in atmospheric models allows for quantitative diagnostics of how water is transported from its source region to its sink region. In this presentation, we review how this methodology is applied to global atmospheric models. We will present several applications of the methodology. In one example, the regional sources of water for the North American Monsoon system are evaluated by tagging the surface evaporation. In another example, the tagged water is used to quantify the global water cycling rate and residence time. We will also discuss the need for more research and the importance of these diagnostics in water cycle studies.

  4. Barium Tagging for nEXO

    NASA Astrophysics Data System (ADS)

    Fudenberg, Daniel; Brunner, Thomas; Varentsov, Victor; Devoe, Ralph; Dilling, Jens; Gratta, Giorgio; nEXO Collaboration

    2015-10-01

    nEXO is a next-generation experiment designed to search for 0 νββ -decay of Xe-136 in a liquid xenon time projection chamber. Positive observation of this decay would determine the neutrino to be a Majorana particle In order to greatly reduce background contributions to this search, the collaboration is developing several ``barium tagging'' techniques to recover and identify the decay daughter, Ba-136. ``Tagging'' may be available for a 2nd phase of nEXO and will push the sensitivity beyond the inverted neutrino-mass hierarchy. Tagging methods in testing for this phase include Ba-ion capture on a probe with identification by resonance ionization laser spectroscopy, and Ba capture in solid xenon on a cold probe with identification by fluorescence. In addition, Ba tagging for a gas-phase detector, appropriate for a later stage, is being tested. Here efficient ion extraction from heavy carrier gases is key. Detailed gas-dynamic and ion transport calculations have been performed to optimize for ion extraction. An apparatus to extract Ba ions from up to 10 bar xenon gas into vacuum using an RF-only funnel has been constructed and demonstrates extraction of ions from noble gases. We will present this system's status along with results of this R&D program.

  5. Discovery STS-131 Mission Landing

    NASA Image and Video Library

    2010-04-20

    STS131-S-086 (20 April 2010) --- The space shuttle Discovery is seen as it lands at the Kennedy Space Center in Cape Canaveral, Florida, on April 20, 2010. Discovery and the STS-131 mission crew, NASA astronauts Alan Poindexter, commander; James P. Dutton Jr., pilot; Dorothy Metcalf-Lindenburger, Rick Mastracchio, Stephanie Wilson, Clayton Anderson and Japanese astronaut Naoko Yamazaki, all mission specialists, returned from their mission to the International Space Station. Photo credit: NASA/Bill Ingalls

  6. Discovery STS-131 Mission Landing

    NASA Image and Video Library

    2010-04-20

    STS131-S-088 (20 April 2010) --- The space shuttle Discovery is seen as it lands at the Kennedy Space Center in Cape Canaveral, Florida, on April 20, 2010. Discovery and the STS-131 mission crew, NASA astronauts Alan Poindexter, commander; James P. Dutton Jr., pilot; Dorothy Metcalf-Lindenburger, Rick Mastracchio, Stephanie Wilson, Clayton Anderson and Japanese astronaut Naoko Yamazaki, all mission specialists, returned from their mission to the International Space Station. Photo credit: NASA/Bill Ingalls

  7. STS-87 Day 08 Highlights

    NASA Technical Reports Server (NTRS)

    1997-01-01

    On this eighth day of the STS-87 mission, the flight crew, Cmdr. Kevin R. Kregel, Pilot Steven W. Lindsey, Mission Specialists Winston E. Scott, Kalpana Chawla, and Takao Doi, and Payload Specialist Leonid K. Kadenyuk take time out from their duties to be interviewed by CNN. As they reach the one week mark in their 16-day flight, the STS-87 crew shift the focus of their efforts towards the variety of science experiments flying on this mission.

  8. 9 CFR 2.54 - Lost tags.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Lost tags. 2.54 Section 2.54 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.54 Lost tags. Each dealer or exhibitor shall be held...

  9. 9 CFR 2.54 - Lost tags.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Lost tags. 2.54 Section 2.54 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.54 Lost tags. Each dealer or exhibitor shall be held...

  10. 9 CFR 2.54 - Lost tags.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Lost tags. 2.54 Section 2.54 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.54 Lost tags. Each dealer or exhibitor shall be held...

  11. 9 CFR 2.54 - Lost tags.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Lost tags. 2.54 Section 2.54 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.54 Lost tags. Each dealer or exhibitor shall be held...

  12. 9 CFR 2.54 - Lost tags.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Lost tags. 2.54 Section 2.54 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.54 Lost tags. Each dealer or exhibitor shall be held...

  13. Hood River PIT-tag interrogation system efficiency study. Annual report of U.S. Geological Survey activities: November 2010-October 2011

    USGS Publications Warehouse

    Jezorek, Ian G.; Connolly, Patrick J.

    2012-01-01

    An additional USGS-CRRL task, under contract number 50150, was to build three antennas for use with Destron-Fearing 2001F-ISO PIT tag readers. These antennas would be 5 used at the East Fork Hood River Acclimation site. They would be placed in the outflow channel to inform managers about the number of PIT tagged steelhead smolts released to the Hood River after a period of acclimation when some mortality and predation might occur. 

  14. Identification on Membrane and Characterization of Phosphoproteins Using an Alkoxide-Bridged Dinuclear Metal Complex as a Phosphate-Binding Tag Molecule

    PubMed Central

    Nakanishi, Tsuyoshi; Ando, Eiji; Furuta, Masaru; Kinoshita, Eiji; Kinoshita-Kikuta, Emiko; Koike, Tohru; Tsunasawa, Susumu; Nishimura, Osamu

    2007-01-01

    We have developed a method for on-membrane direct identification of phosphoproteins, which are detected by a phosphate-binding tag (Phos-tag) that has an affinity to phosphate groups with a chelated Zn2+ ion. This rapid profiling approach for phosphoproteins combines chemical inkjet technology for microdispensing of reagents onto a tiny region of target proteins with mass spectrometry for on-membrane digested peptides. Using this method, we analyzed human epidermoid carcinoma cell lysates of A-431 cells stimulated with epidermal growth factor, and identified six proteins with intense signals upon affinity staining with the phosphate-binding tag. It was already known that these proteins are phosphorylated, and our new approach proved to be effective at rapid profiling of phosphoproteins. Furthermore, we tried to determine their phosphorylation sites by MS/MS analysis after in-gel digestion of the corresponding spots on the 2DE gel to the rapid on-membrane identifications. As one example of use of information gained from the rapid-profiling approach, we successfully characterized a phosphorylation site at Ser-113 on prostaglandin E synthase 3. PMID:18166671

  15. Electronic tagging and integrated product intelligence

    NASA Astrophysics Data System (ADS)

    Swerdlow, Martin; Weeks, Brian

    1996-03-01

    The advent of 'intelligent,' electronic data bearing tags is set to revolutionize the way industrial and retail products are identified and tracked throughout their life cycles. The dominant system for unique identification today is the bar code, which is based on printed symbology and regulated by the International Article Numbering Association. Bar codes provide users with significant operational advantages and generate considerable added value to packaging companies, product manufacturers, distributors and retailers, across supply chains in many different sectors, from retailing, to baggage handling and industrial components, e.g., for vehicles or aircraft. Electronic tags offer the potential to: (1) record and store more complex data about the product or any modifications which occur during its life cycle; (2) access (and up-date) stored data in real time in a way which does not involve contact with the product or article; (3) overcome the limitations imposed by systems which rely on line-of-sight access to stored data. Companies are now beginning to consider how electronic data tags can be used, not only to improve the efficiency of their supply chain processes, but also to revolutionize the way they do business. This paper reviews the applications and business opportunities for electronic tags and outlines CEST's strategy for achieving an 'open' standard which will ensure that tags from different vendors can co-exist on an international basis.

  16. Radiostrontium contamination of soil and vegetation within the Semipalatinsk test site.

    PubMed

    Howard, B J; Semioschkina, N; Voigt, G; Mukusheva, M; Clifford, J

    2004-12-01

    The Semipalatinsk nuclear test site (STS) in the Republic of Kazakhstan was an important site for testing atomic bombs and other civil and military nuclear devices of the former Soviet Union. Results are presented from investigations on the extent of radiostrontium contamination in soils and vegetation at the technical areas of the STS, where the tests were conducted and in pastures used by farmers for grazing animals or for hay production. Our data are compared with those reported largely in the recent Russian language literature that has been reviewed. The extent of (90)Sr contamination of soil is highly variable over the STS with the highest values associated with the technical areas, particularly the Degelen mountains. Recently measured values in both the present data and the Russian language literature confirm the relatively high current contamination of soil and vegetation in the vicinity of tunnels and associated watercourses in the Degelen area. The proportion of (90)Sr in soil which could not be extracted with 6 M HCl was only an average of 20%, which is low compared to other test site areas and possibly indicates a relatively high mobility in this area, because the (90)Sr is derived from leakage from explosion tunnels along watercourses rather than being associated with fused silicates. A comparison of relative activity concentrations in soil and vegetation suggests that the transfer of (90)Sr to vegetation on the STS is high compared to that of (137)Cs and plutonium.

  17. Preparation of dart tags for use in the field

    USGS Publications Warehouse

    Higham, Joseph R.

    1966-01-01

    Tagging in the field requires an efficient method of preparing the tags for dispensation under a wide range of conditions. The method described here was very efficient in an extensive tagging program on Oahe Reservoir, South Dakota.

  18. Passive UHF RFID Tag with Multiple Sensing Capabilities

    PubMed Central

    Fernández-Salmerón, José; Rivadeneyra, Almudena; Martínez-Martí, Fernando; Capitán-Vallvey, Luis Fermín; Palma, Alberto J.; Carvajal, Miguel A.

    2015-01-01

    This work presents the design, fabrication, and characterization of a printed radio frequency identification tag in the ultra-high frequency band with multiple sensing capabilities. This passive tag is directly screen printed on a cardboard box with the aim of monitoring the packaging conditions during the different stages of the supply chain. This tag includes a commercial force sensor and a printed opening detector. Hence, the force applied to the package can be measured as well as the opening of the box can be detected. The architecture presented is a passive single-chip RFID tag. An electronic switch has been implemented to be able to measure both sensor magnitudes in the same access without including a microcontroller or battery. Moreover, the chip used here integrates a temperature sensor and, therefore, this tag provides three different parameters in every reading. PMID:26506353

  19. A SNAP-Tagged Derivative of HIV-1—A Versatile Tool to Study Virus-Cell Interactions

    PubMed Central

    Eckhardt, Manon; Anders, Maria; Muranyi, Walter; Heilemann, Mike; Krijnse-Locker, Jacomine; Müller, Barbara

    2011-01-01

    Fluorescently labeled human immunodeficiency virus (HIV) derivatives, combined with the use of advanced fluorescence microscopy techniques, allow the direct visualization of dynamic events and individual steps in the viral life cycle. HIV proteins tagged with fluorescent proteins (FPs) have been successfully used for live-cell imaging analyses of HIV-cell interactions. However, FPs display limitations with respect to their physicochemical properties, and their maturation kinetics. Furthermore, several independent FP-tagged constructs have to be cloned and characterized in order to obtain spectral variations suitable for multi-color imaging setups. In contrast, the so-called SNAP-tag represents a genetically encoded non-fluorescent tag which mediates specific covalent coupling to fluorescent substrate molecules in a self-labeling reaction. Fusion of the SNAP-tag to the protein of interest allows specific labeling of the fusion protein with a variety of synthetic dyes, thereby offering enhanced flexibility for fluorescence imaging approaches. Here we describe the construction and characterization of the HIV derivative HIVSNAP, which carries the SNAP-tag as an additional domain within the viral structural polyprotein Gag. Introduction of the tag close to the C-terminus of the matrix domain of Gag did not interfere with particle assembly, release or proteolytic virus maturation. The modified virions were infectious and could be propagated in tissue culture, albeit with reduced replication capacity. Insertion of the SNAP domain within Gag allowed specific staining of the viral polyprotein in the context of virus producing cells using a SNAP reactive dye as well as the visualization of individual virions and viral budding sites by stochastic optical reconstruction microscopy. Thus, HIVSNAP represents a versatile tool which expands the possibilities for the analysis of HIV-cell interactions using live cell imaging and sub-diffraction fluorescence microscopy. PMID:21799764

  20. Perception without self-matching in conditional tag based cooperation.

    PubMed

    McAvity, David M; Bristow, Tristen; Bunker, Eric; Dreyer, Alex

    2013-09-21

    We consider a model for the evolution of cooperation in a population where individuals may have one of a number of different heritable and distinguishable markers or tags. Individuals interact with each of their neighbors on a square lattice by either cooperating by donating some benefit at a cost to themselves or defecting by doing nothing. The decision to cooperate or defect is contingent on each individual's perception of its interacting partner's tag. Unlike in other tag-based models individuals do not compare their own tag to that of their interaction partner. That is, there is no self-matching. When perception is perfect the cooperation rate is substantially higher than in the usual spatial prisoner's dilemma game when the cost of cooperation is high. The enhancement in cooperation is positively correlated with the number of different tags. The more diverse a population is the more cooperative it becomes. When individuals start with an inability to perceive tags the population evolves to a state where individuals gain at least partial perception. With some reproduction mechanisms perfect perception evolves, but with others the ability to perceive tags is imperfect. We find that perception of tags evolves to lower levels when the cost of cooperation is higher. Copyright © 2013 Elsevier Ltd. All rights reserved.

  1. Fully printed flexible and disposable wireless cyclic voltammetry tag.

    PubMed

    Jung, Younsu; Park, Hyejin; Park, Jin-Ah; Noh, Jinsoo; Choi, Yunchang; Jung, Minhoon; Jung, Kyunghwan; Pyo, Myungho; Chen, Kevin; Javey, Ali; Cho, Gyoujin

    2015-01-29

    A disposable cyclic voltammetry (CV) tag is printed on a plastic film by integrating wireless power transmitter, polarized triangle wave generator, electrochemical cell and signage through a scalable gravure printing method. By proximity of 13.56 MHz RF reader, the printed CV tag generates 320 mHz of triangular sweep wave from +500 mV to -500 mV which enable to scan a printed electrochemical cell in the CV tag. By simply dropping any specimen solution on the electrochemical cell in the CV tag, the presence of solutes in the solution can be detected and shown on the signage of the CV tag in five sec. 10 mM of N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) was used as a standard solute to prove the working concept of fully printed disposable wireless CV tag. Within five seconds, we can wirelessly diagnose the presence of TMPD in the solution using the CV tag in the proximity of the 13.56 MHz RF reader. This fully printed and wirelessly operated flexible CV tag is the first of its kind and marks the path for the utilization of inexpensive and disposable wireless electrochemical sensor systems for initial diagnose hazardous chemicals and biological molecules to improve public hygiene and health.

  2. Fully printed flexible and disposable wireless cyclic voltammetry tag

    NASA Astrophysics Data System (ADS)

    Jung, Younsu; Park, Hyejin; Park, Jin-Ah; Noh, Jinsoo; Choi, Yunchang; Jung, Minhoon; Jung, Kyunghwan; Pyo, Myungho; Chen, Kevin; Javey, Ali; Cho, Gyoujin

    2015-01-01

    A disposable cyclic voltammetry (CV) tag is printed on a plastic film by integrating wireless power transmitter, polarized triangle wave generator, electrochemical cell and signage through a scalable gravure printing method. By proximity of 13.56 MHz RF reader, the printed CV tag generates 320 mHz of triangular sweep wave from +500 mV to -500 mV which enable to scan a printed electrochemical cell in the CV tag. By simply dropping any specimen solution on the electrochemical cell in the CV tag, the presence of solutes in the solution can be detected and shown on the signage of the CV tag in five sec. 10 mM of N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) was used as a standard solute to prove the working concept of fully printed disposable wireless CV tag. Within five seconds, we can wirelessly diagnose the presence of TMPD in the solution using the CV tag in the proximity of the 13.56 MHz RF reader. This fully printed and wirelessly operated flexible CV tag is the first of its kind and marks the path for the utilization of inexpensive and disposable wireless electrochemical sensor systems for initial diagnose hazardous chemicals and biological molecules to improve public hygiene and health.

  3. 77 FR 51761 - Proposed Information Collection; Comment Request; Groundfish Tagging Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-27

    ... required by the Paperwork Reduction Act of 1995. DATES: Written comments must be submitted on or before... are two general categories of tags. Simple plastic tags (spaghetti tags) are external tags... fish. Archival tags are microchips with sensors encased in plastic cylinders that record the depth...

  4. Tagging Efficiency for Nuclear Physics Measurements at MAX-lab

    NASA Astrophysics Data System (ADS)

    Miller, Nevin; Elofson, David; Lewis, Codie; O'Brien, Erin; Buggelli, Kelsey; O'Connor, Kyle; O'Rielly, Grant; Maxtagg Team

    2014-09-01

    A careful study of the tagging efficiency during measurements of near threshold pion photoproduction and high energy Compton scattering has been performed. These experiments are being done at the MAX-lab tagged photon Facility during the June 2014 run period. The determination of the final results from these experiments depends on knowledge of the incident photon flux. The tagging efficiency is a critical part of the photon flux calculation. In addition to daily measurements of the tagging efficiency, a beam monitor was used during the production data runs to monitor the relative tagging efficiency. Two trigger types were used in the daily measurements; one was a logical OR from the tagger array and the other was from the Pb-glass photon detector. Investigations were made to explore the effect of the different trigger conditions and the differences between single and multi hit TDCs on the tagging efficiency. In addition the time evolution and overall uncertainty in the tagging efficiency for each tagger channel was determined. The results will be discussed.

  5. Evaluation of Fish Movements, Migration Patterns, and Population Abundance with Streamwidth PIT Tag Interrogation Systems, Final Report 2002.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Zydlewski, Gayle; Winter, Christiane; McClanahan, Dee

    2003-02-01

    Two remote Streamwidth PIT tag Interrogation systems (SPIs) were operated continuously for over one year to test the feasibility of these systems for generating movement, migration, survival and smolt production estimates for salmonids. A total of 1,588 juvenile (< 100 mm FL) naturally produced salmonids (7 coho salmon, 482 cutthroat trout, and 1,099 steelhead) were PIT tagged above the upstream-most SPI (9 sites approximately 1 linear km each) in Fall 2001. Age at tagging for wild caught cutthroat and steelhead was 1 year. SPIs were operating before any PIT tagged fish were released in the creek. Over 390,000 detections weremore » recorded from October 2001 to 31 July 2002. Efficiencies were site dependent, but overall detection efficiency for the creek was 97% with 95% confidence intervals of 91-100%. PIT tag detection efficiency ranged from 55-100% depending on the SPI and varied throughout the year with average efficiencies of 73% and 89%. SPI efficiency of PIT tag detection was not completely dependent on electronics noise levels or environmental conditions. Fish from all tagging locations were detected at the SPIs. Steelhead and cutthroat trout were primarily detected moving in the Spring (April-June) coincident with the anticipated smolt migration. Steelhead were also detected moving past SPIs at lower numbers in the Fall and Winter. Travel time between SPIs (downstream movement) was highly dependent on time of year. Travel time in the Spring was significantly faster (34.4 {+-} 7.0 hours) for all species than during any other time of year (763.1 {+-} 267.0 hours). Steelhead and cutthroat migrating in the Spring were the same age as those that did not migrate in the Spring. Peak of steelhead migration recorded at the two SPIs was 5/11 and 5/12 and the peak in the screw trap was recorded on 5/17. Steelhead smolt production estimates using SPIs (3,802 with 95% confidence intervals of 3,440 - 4,245) was similar to those using more standard screw trap methods

  6. Evaluation of Fish Movements, Migration Patterns and Populations Abundance with Streamwidth PIT Tag Interrogation Systems, Final Report 2002.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Zydlewski, Gayle B.; Casey, Sean

    2003-02-01

    Two remote Streamwidth PIT tag Interrogation systems (SPIs) were operated continuously for over one year to test the feasibility of these systems for generating movement, migration, survival and smolt production estimates for salmonids. A total of 1,588 juvenile (< 100 mm FL) naturally produced salmonids (7 coho salmon, 482 cutthroat trout, and 1,099 steelhead) were PIT tagged above the upstream-most SPI (9 sites approximately 1 linear km each) in Fall 2001. Age at tagging for wild caught cutthroat and steelhead was 1 year. SPIs were operating before any PIT tagged fish were released in the creek. Over 390,000 detections weremore » recorded from October 2001 to 31 July 2002. Efficiencies were site dependent, but overall detection efficiency for the creek was 97% with 95% confidence intervals of 91-100%. PIT tag detection efficiency ranged from 55-100% depending on the SPI and varied throughout the year with average efficiencies of 73% and 89%. SPI efficiency of PIT tag detection was not completely dependent on electronics noise levels or environmental conditions. Fish from all tagging locations were detected at the SPIs. Steelhead and cutthroat trout were primarily detected moving in the Spring (April-June) coincident with the anticipated smolt migration. Steelhead were also detected moving past SPIs at lower numbers in the Fall and Winter. Travel time between SPIs (downstream movement) was highly dependent on time of year. Travel time in the Spring was significantly faster (34.4 {+-} 7.0 hours) for all species than during any other time of year (763.1 {+-} 267.0 hours). Steelhead and cutthroat migrating in the Spring were the same age as those that did not migrate in the Spring. Peak of steelhead migration recorded at the two SPIs was 5/11 and 5/12 and the peak in the screw trap was recorded on 5/17. Steelhead smolt production estimates using SPIs (3,802 with 95% confidence intervals of 3,440-4,245) was similar to those using more standard screw trap methods

  7. Improving Attachments of Remotely-Deployed Dorsal Fin-Mounted Tags: Tissue Structure, Hydrodynamics, in situ Performance, and Tagged-Animal Follow-up

    DTIC Science & Technology

    2014-09-30

    TERM GOALS We recently developed small satellite-linked telemetry tags that are anchored with small attachment darts to the dorsal fins of small ...monitor the movements of numerous species not previously accessible because they were too large or difficult to capture safely, but too small for tags...cetaceans that provides the data needed to answer critical conservation and management questions without an adverse effect on the tagged animal. Therefore

  8. STS-111 Crew in white room during TCDT

    NASA Technical Reports Server (NTRS)

    2002-01-01

    KENNEDY SPACE CENTER, FLA. -- In the White Room, Launch Pad 39A, the STS-111 and Expedition 5 crews pose in front of the entry into Space Shuttle Endeavour. From left are Expedition 5 crew member Sergei Treschev and Commander Valeri Korzun, with the Russian Space Agency; STS-111 Mission Specialist Philippe Perrin, with the French Space Agency; Commander Kenneth Cockrell and Pilot Paul Lockhart; Expedition 5 crew member Peggy Whitson; and Mission Specialist Franklin Chang-Diaz. The crews are taking part in Terminal Countdown Demonstration Test activities at the pad, which include emergency egress training and a simulated launch countdown. The mission is Utilization Flight 2, carrying supplies and equipment to the International Space Station, the Mobile Base System, which will be installed on the Mobile Transporter to complete the Canadian Mobile Servicing System, or MSS, and a replacement wrist/roll joint for Canadarm 2. The mechanical arm will then have the capability to 'inchworm' from the U.S. Lab Destiny to the MSS and travel along the truss to work sites. Expedition 5 will travel to the Station on Endeavour as the replacement crew for Expedition 4, who will return to Earth aboard the orbiter. Launch is scheduled for May 30, 2002.

  9. Space Shuttle Atlantis Landing / STS-129 Mission

    NASA Image and Video Library

    2009-11-27

    PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - With landing gear down, space shuttle Atlantis approaches landing on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kim Shiflett

  10. Space Shuttle Atlantis Landing / STS-129 Mission

    NASA Image and Video Library

    2009-11-27

    PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - With drag chute unfurled, space shuttle Atlantis lands on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kim Shiflett

  11. Space Shuttle Atlantis Landing / STS-129 Mission

    NASA Image and Video Library

    2009-11-27

    PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - The drag chute unfurls to slow space shuttle Atlantis for landing on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Sandra Joseph

  12. Space Shuttle Atlantis Landing / STS-129 Mission

    NASA Image and Video Library

    2009-11-27

    PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - The drag chute unfurls as space shuttle Atlantis lands on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kim Shiflett

  13. Space Shuttle Atlantis Landing / STS-129 Mission

    NASA Image and Video Library

    2009-11-27

    PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - The drag chute unfurls to slow space shuttle Atlantis for landing on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

  14. Space Shuttle Atlantis Landing / STS-129 Mission

    NASA Image and Video Library

    2009-11-27

    PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - Space shuttle Atlantis kicks up dust as it touches down on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kim Shiflett

  15. Space Shuttle Atlantis Landing / STS-129 Mission

    NASA Image and Video Library

    2009-11-27

    PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - Streams of smoke trail from the main landing gear tires as space shuttle Atlantis touches down on Runway 33 at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida after 11 days in space, completing the 4.5-million-mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

  16. Space Shuttle Atlantis Landing / STS-129 Mission

    NASA Image and Video Library

    2009-11-27

    PHOTO CREDIT: NASA or National Aeronautics and Space Administration CAPE CANAVERAL, Fla. - A fire and rescue truck is in place beside Runway 33 if needed to support the landing of space shuttle Atlantis at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. After 11 days in space, Atlantis completed the 4.5-million mile STS-129 mission on orbit 171. Main gear touchdown was at 9:44:23 a.m. EDT. Nose gear touchdown was at 9:44:36 a.m., and wheels stop was at 9:45:05 a.m. Aboard Atlantis are Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr.; and Expedition 20 and 21 Flight Engineer Nicole Stott who spent 87 days aboard the International Space Station. STS-129 is the final space shuttle Expedition crew rotation flight on the manifest. On STS-129, the crew delivered 14 tons of cargo to the orbiting laboratory, including two ExPRESS Logistics Carriers containing spare parts to sustain station operations after the shuttles are retired next year. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jack Pfaller

  17. sts132-s-006

    NASA Image and Video Library

    2010-05-14

    STS132-S-006 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

  18. STS095-s-001

    NASA Image and Video Library

    1998-06-01

    STS095-S-001 (June 1998) --- The STS-95 patch, designed by the crew, is intended to reflect the scientific, engineering, and historic elements of the mission. The space shuttle Discovery is shown rising over the sunlit Earth limb, representing the global benefits of the mission science and the solar science objectives of the Spartan Satellite. The bold number "7" signifies the seven members of Discovery's crew and also represents a historical link to the original seven Mercury astronauts. The STS-95 crew member John Glenn's first orbital flight is represnted by the Friendship 7 capsule. The rocket plumes symbolize the three major fields of science represented by the mission payloads: microgravity material science, medical research for humans on Earth and in space, and astronomy. The NASA insignia design for space shuttle flights is reserved for use by the astronauts and for other official use as the NASA Administrator may authorize. Public availability has been approved only in the forms of illustrations by the various news media. When and if there is any change in this policy, which is not anticipated, the change will be publicly announced. Photo credit: NASA

  19. sts132-s-009

    NASA Image and Video Library

    2010-05-14

    STS132-S-009 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

  20. sts132-s-008

    NASA Image and Video Library

    2010-05-14

    STS132-S-008 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

  1. sts132-s-007

    NASA Image and Video Library

    2010-05-14

    STS132-S-007 (14 May 2010) --- Space shuttle Atlantis and its six-member STS-132 crew head toward Earth orbit and rendezvous with the International Space Station. Liftoff was at 2:20 p.m. (EDT) on May 14, 2010, from launch pad 39A at NASA's Kennedy Space Center. Onboard are NASA astronauts Ken Ham, commander; Tony Antonelli, pilot; Garrett Reisman, Michael Good, Steve Bowen and Piers Sellers, all mission specialists. The crew will deliver the Russian-built Mini-Research Module 1 (MRM-1) to the International Space Station. Named Rassvet, Russian for "dawn," the module is the second in a series of new pressurized components for Russia and will be permanently attached to the Earth-facing port of the Zarya Functional Cargo Block (FGB). Rassvet will be used for cargo storage and will provide an additional docking port to the station. Also aboard Atlantis is an Integrated Cargo Carrier, or ICC, an unpressurized flat bed pallet and keel yoke assembly used to support the transfer of exterior cargo from the shuttle to the station. STS-132 is the 34th mission to the station and the last scheduled flight for Atlantis.

  2. Annual Outcomes With Transcatheter Valve Therapy: From the STS/ACC TVT Registry.

    PubMed

    Holmes, David R; Nishimura, Rick A; Grover, Frederick L; Brindis, Ralph G; Carroll, John D; Edwards, Fred H; Peterson, Eric D; Rumsfeld, John S; Shahian, David M; Thourani, Vinod H; Tuzcu, E Murat; Vemulapalli, Sreekanth; Hewitt, Kathleen; Michaels, Joan; Fitzgerald, Susan; Mack, Michael J

    2016-02-01

    The Society of Thoracic Surgeons (STS)/American College of Cardiology (ACC) Transcatheter Valve Therapy (TVT) Registry has been a joint initiative of the STS and the ACC in concert with multiple stakeholders. The TVT Registry has important information regarding patient selection, delivery of care, science, education, and research in the field of structural valvular heart disease. This report provides an overview on current U.S. TVT practice and trends. The emphasis is on demographics, in-hospital procedural characteristics, and outcomes of patients having transcatheter aortic valve replacement (TAVR) performed at 348 U.S. centers. The TVT Registry captured 26,414 TAVR procedures as of December 31, 2014. Temporal trends between 2012 and 2013 versus 2014 were compared. Comparison of the 2 time periods reveals that TAVR patients remain elderly (mean age 82 years), with multiple comorbidities, reflected by a high mean STS predicted risk of mortality (STS PROM) for surgical valve replacement (8.34%), were highly symptomatic (New York Heart Association functional class III/IV in 82.5%), frail (slow 5-m walk test in 81.6%), and have poor self-reported health status (median baseline Kansas City Cardiomyopathy Questionnaire score of 39.1). Procedure performance is changing, with an increased use of moderate sedation (from 1.6% to 5.1%) and increase in femoral access using percutaneous techniques (66.8% in 2014). Vascular complication rates are decreasing (from 5.6% to 4.2%), whereas site-reported stroke rates remain stable at 2.2%. The TVT Registry provides important information on characteristics and outcomes of TAVR in contemporary U.S. clinical practice. It can be used to identify trends in practice and opportunities for quality improvement.

  3. sts093-s-005

    NASA Image and Video Library

    2009-09-23

    STS93-S-005 (23 July 1999) --- The Space Shuttle Columbia lifts off from Launch Pad 39B to begin the five-day STS-93 mission in this 70mm frame. After two unsuccessful attempts earlier in the week, liftoff occurred at 12:31 a.m. (EDT), July 23, 1999. Only hours after this picture was taken, the five-member crew released the Chandra X-Ray Observatory into orbit. Onboard were astronauts Eileen M. Collins, first woman shuttle mission commander; Jeffrey S. Ashby, pilot; and Steven A. Hawley, Catherine G. Coleman and Michel Tognini, all mission specialists. Tognini represents the Centre National d'Etudes Spatiales (CNES) of France.

  4. sts093-s-007

    NASA Image and Video Library

    2009-09-23

    STS93-S-007 (23 July 1999) --- Framed by Florida foliage in this night time scene, the Space Shuttle Columbia lifts off from Launch Pad 39B to begin the five-day STS-93 mission. After two unsuccessful attempts earlier in the week, liftoff occurred at 12:31 a.m. (EDT), July 23, 1999. Only hours after this picture was taken, the five-member crew released the Chandra X-Ray Observatory into orbit. Onboard were astronauts Eileen M. Collins, first woman shuttle commander; Jeffrey S. Ashby, pilot; and Steven A. Hawley, Catherine G. Coleman and Michel Tognini, all mission specialists. Tognini represents the Centre National d'Etudes Spatiales (CNES) of France.

  5. STS-6 MISSION OPERATIONS CONTROL ROOM (MOCR) ACTIVITIES - DAY 5 - JSC

    NASA Image and Video Library

    1983-04-09

    Various views of STS-6 MOCR activities during Day-5 with Vice-Pres. George Bush, Cap Communicator Bridges, JSC Director Gerald Griffin, Eugene F. Kranz, NASA Admin. James M. Beggs, Cap Com Astronaut O'Connor, Flight Directors Jay H. Greene, Gary E. Coen, and Harold Draughon. 1. BUSH, GEORGE, VICE-PRES. - STS-6 MOCR 2. DIR. GRIFFIN, GERALD D. - STS-6 MOCR 3. ADMIN. BEGGS, JAMES M. - STS-6 MOCR 4. FLT. DIRECTORS - STS-6 JSC, HOUSTON, TX Also available in 35 CN

  6. AFFTC commander Brig. Gen. Curtis Bedke experienced a Shuttle approach and landing in NASA's Shuttle Training Aircraft from STS-114 commander Eileen Collins

    NASA Image and Video Library

    2005-03-29

    Brig. Gen. Curtis Bedke, commander of the Air Force Flight Test Center at Edwards Air Force Base, received some first-hand insight on how to fly a Space Shuttle approach and landing, courtesy of NASA astronaut and STS-114 mission commander Eileen Collins. The series of proficiency flights in NASA's modified Grumman Gulfstream-II Shuttle Training Aircraft were in preparation for the STS-114 mission with the shuttle Discovery. Although NASA's Kennedy Space Center in Florida is the primary landing site for Space Shuttle missions, flight crews also practice the shuttle's steep approach and landing at Edwards in case weather or other situations preclude a landing at the Florida site and force a diversion to Edwards AFB.

  7. INFLIGHT - STS-7

    NASA Image and Video Library

    1983-06-27

    Full views of "Challenger" in Space, taken by the Shuttle Pallet Satellite (SPAS), also views of Cargo Bay and Remote Manipulator System (RMS) extended. 1. SHUTTLE - RMS (STS-7) Also available in 4x5 CN

  8. Electronic archival tags provide first glimpse of bathythermal habitat use by free-ranging adult lake sturgeon Acipenser fulvescens

    USGS Publications Warehouse

    Briggs, Andrew S.; Hondorp, Darryl W.; Quinlan, Henry R.; Boase, James C.; Mohr, Lloyd C.

    2016-01-01

    Information on lake sturgeon (Acipenser fulvescens) depth and thermal habitat use during non-spawning periods is unavailable due to the difficulty of observing lake sturgeon away from shallow water spawning sites. In 2002 and 2003, lake sturgeon captured in commercial trap nets near Sarnia, Ontario were implanted with archival tags and released back into southern Lake Huron. Five of the 40 tagged individuals were recaptured and were at large for 32, 57, 286, 301, and 880 days. Temperatures and depths recorded by archival tags ranged from 0 to 23.5 ºC and 0.1 to 42.4 m, respectively. For the three lake sturgeon that were at large for over 200 days, temperatures occupied emulated seasonal fluctuations. Two of these fish occupied deeper waters during winter than summer while the other occupied similar depths during non-spawning periods. This study provides important insight into depth and thermal habitat use of lake sturgeon throughout the calendar year along with exploring the feasibility of using archival tags to obtain important physical habitat attributes during non-spawning periods.

  9. Launch of Space Shuttle Atlantis / STS-129 Mission

    NASA Image and Video Library

    2009-11-16

    CAPE CANAVERAL, Fla. - Space shuttle Atlantis launches through the clouds from Launch Pad 39A on a balmy Florida afternoon at NASA's Kennedy Space Center. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

  10. Launch of Space Shuttle Atlantis / STS-129 Mission

    NASA Image and Video Library

    2009-11-16

    CAPE CANAVERAL, Fla. - Space shuttle Atlantis cuts its way through the blue skies over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Jim Grossmann

  11. STA Flying Weather Reconnaissance / STS-134 Mission

    NASA Image and Video Library

    2011-06-01

    STS134-S-067 (1 June 2011) --- Astronaut Rick Sturckow flies weather reconnaissance in a Shuttle Training Aircraft over NASA's Kennedy Space Center in Florida to assess conditions before space shuttle Endeavour returns to Earth for the final time. Weather was observed "go" and Endeavour glided to a stop on the Shuttle Landing Facility's Runway 15 at 2:35 a.m. EDT, bringing an end to the STS-134 mission. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA

  12. Sequence tagging reveals unexpected modifications in toxicoproteomics

    PubMed Central

    Dasari, Surendra; Chambers, Matthew C.; Codreanu, Simona G.; Liebler, Daniel C.; Collins, Ben C.; Pennington, Stephen R.; Gallagher, William M.; Tabb, David L.

    2010-01-01

    Toxicoproteomic samples are rich in posttranslational modifications (PTMs) of proteins. Identifying these modifications via standard database searching can incur significant performance penalties. Here we describe the latest developments in TagRecon, an algorithm that leverages inferred sequence tags to identify modified peptides in toxicoproteomic data sets. TagRecon identifies known modifications more effectively than the MyriMatch database search engine. TagRecon outperformed state of the art software in recognizing unanticipated modifications from LTQ, Orbitrap, and QTOF data sets. We developed user-friendly software for detecting persistent mass shifts from samples. We follow a three-step strategy for detecting unanticipated PTMs in samples. First, we identify the proteins present in the sample with a standard database search. Next, identified proteins are interrogated for unexpected PTMs with a sequence tag-based search. Finally, additional evidence is gathered for the detected mass shifts with a refinement search. Application of this technology on toxicoproteomic data sets revealed unintended cross-reactions between proteins and sample processing reagents. Twenty five proteins in rat liver showed signs of oxidative stress when exposed to potentially toxic drugs. These results demonstrate the value of mining toxicoproteomic data sets for modifications. PMID:21214251

  13. STS-32 view of the moon setting over the Earth's limb

    NASA Image and Video Library

    1990-01-20

    STS-32 crew took this view of the moon setting over the Earth's limb. Near the center is a semi-vortex in the clouds - a storm system in the early stages of formation. The moon's image is distorted due to refraction through the Earth's atmosphere. The near side of the moon is visible showing the vast area of the moon's western seas (Mare Occidental), Apollo landing sites: Apollo 14 at Fra Mauro and Apollo 16 at Central Highlands near Descartes.

  14. STS-32 view of the moon setting over the Earth's limb

    NASA Technical Reports Server (NTRS)

    1990-01-01

    STS-32 crew took this view of the moon setting over the Earth's limb. Near the center is a semi-vortex in the clouds - a storm system in the early stages of formation. The moon's image is distorted due to refraction through the Earth's atmosphere. The near side of the moon is visible showing the vast area of the moon's western seas (Mare Occidental), Apollo landing sites: Apollo 14 at Fra Mauro and Apollo 16 at Central Highlands near Descartes.

  15. High-level expression of soluble recombinant proteins in Escherichia coli using an HE-maltotriose-binding protein fusion tag.

    PubMed

    Han, Yingqian; Guo, Wanying; Su, Bingqian; Guo, Yujie; Wang, Jiang; Chu, Beibei; Yang, Guoyu

    2018-02-01

    Recombinant proteins are commonly expressed in prokaryotic expression systems for large-scale production. The use of genetically engineered affinity and solubility enhancing fusion proteins has increased greatly in recent years, and there now exists a considerable repertoire of these that can be used to enhance the expression, stability, solubility, folding, and purification of their fusion partner. Here, a modified histidine tag (HE) used as an affinity tag was employed together with a truncated maltotriose-binding protein (MBP; consisting of residues 59-433) from Pyrococcus furiosus as a solubility enhancing tag accompanying a tobacco etch virus protease-recognition site for protein expression and purification in Escherichia coli. Various proteins tagged at the N-terminus with HE-MBP(Pyr) were expressed in E. coli BL21(DE3) cells to determine expression and solubility relative to those tagged with His6-MBP or His6-MBP(Pyr). Furthermore, four HE-MBP(Pyr)-fused proteins were purified by immobilized metal affinity chromatography to assess the affinity of HE with immobilized Ni 2+ . Our results showed that HE-MBP(Pyr) represents an attractive fusion protein allowing high levels of soluble expression and purification of recombinant protein in E. coli. Copyright © 2017 Elsevier Inc. All rights reserved.

  16. STS-105 Flight Control Team Photo

    NASA Image and Video Library

    2001-08-16

    JSC2001-02228 (16 August 2001) --- The members of the STS-105/ISS 7A.1 Planning team pose for a group portrait in the shuttle flight control room (WFCR) in Houston’s Mission Control Center (MCC). Astronaut Robert L. Curbeam, Jr., spacecraft communicator (CAPCOM), stands behind the STS-105 mission logo. Flight director Bryan Austin is visible in the front row on the far right.

  17. Earth observation taken during STS-102

    NASA Image and Video Library

    2001-06-14

    STS102-342-024 (8-21 March 2001)--- The largest percentage of astronaut out-the-window photography is scientific in nature. However, occasionally scenes such as this one showing the moon over Earth's airglow are irresistable for crew members with cameras. The 35mm scene was recorded by one of the STS-102 astronauts from the aft flight deck of the Space Shuttle Discovery.

  18. STS-121 crew visits SSC

    NASA Technical Reports Server (NTRS)

    2006-01-01

    Astronauts Steve Lindsey (left), Stephanie Wilson, Lisa Nowak and Piers Sellers meet with employees at NASA Stennis Space Center. The crewmembers on NASA's space shuttle mission STS-121, which launched July 4, 2006, thanked SSC's workers for their dedication and safe work history. `We feel blessed that you are a part of the NASA family,' Wilson said. All four expressed gratitude for the reliability of the space shuttle's main engines, which helped propel the STS-121 crew into orbit on their 13-day mission.

  19. STS-121 crew visits SSC

    NASA Image and Video Library

    2006-09-25

    Astronauts Steve Lindsey (left), Stephanie Wilson, Lisa Nowak and Piers Sellers meet with employees at NASA Stennis Space Center. The crewmembers on NASA's space shuttle mission STS-121, which launched July 4, 2006, thanked SSC's workers for their dedication and safe work history. `We feel blessed that you are a part of the NASA family,' Wilson said. All four expressed gratitude for the reliability of the space shuttle's main engines, which helped propel the STS-121 crew into orbit on their 13-day mission.

  20. STS-109 inflight crew portrait

    NASA Image and Video Library

    2002-03-11

    STS109-E-6032 (11 March 2002) --- On the Space Shuttle Columbia’s mid deck, the crewmembers for the STS-109 mission pose for the traditional in-flight portrait. From the left (front row), are astronauts Nancy J. Currie, mission specialist, Scott D. Altman, mission commander, and Duane G. Carey, pilot. From the left (back row), are astronauts John M. Grunsfeld, payload commander, and Richard M. Linnehan, James H. Newman, and Michael J. Massimino, all mission specialists. The image was recorded with a digital still camera.

  1. Fully printed flexible and disposable wireless cyclic voltammetry tag

    PubMed Central

    Jung, Younsu; Park, Hyejin; Park, Jin-Ah; Noh, Jinsoo; Choi, Yunchang; Jung, Minhoon; Jung, Kyunghwan; Pyo, Myungho; Chen, Kevin; Javey, Ali; Cho, Gyoujin

    2015-01-01

    A disposable cyclic voltammetry (CV) tag is printed on a plastic film by integrating wireless power transmitter, polarized triangle wave generator, electrochemical cell and signage through a scalable gravure printing method. By proximity of 13.56 MHz RF reader, the printed CV tag generates 320 mHz of triangular sweep wave from +500 mV to −500 mV which enable to scan a printed electrochemical cell in the CV tag. By simply dropping any specimen solution on the electrochemical cell in the CV tag, the presence of solutes in the solution can be detected and shown on the signage of the CV tag in five sec. 10 mM of N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD) was used as a standard solute to prove the working concept of fully printed disposable wireless CV tag. Within five seconds, we can wirelessly diagnose the presence of TMPD in the solution using the CV tag in the proximity of the 13.56 MHz RF reader. This fully printed and wirelessly operated flexible CV tag is the first of its kind and marks the path for the utilization of inexpensive and disposable wireless electrochemical sensor systems for initial diagnose hazardous chemicals and biological molecules to improve public hygiene and health. PMID:25630250

  2. Method for nonlinear optimization for gas tagging and other systems

    DOEpatents

    Chen, Ting; Gross, Kenny C.; Wegerich, Stephan

    1998-01-01

    A method and system for providing nuclear fuel rods with a configuration of isotopic gas tags. The method includes selecting a true location of a first gas tag node, selecting initial locations for the remaining n-1 nodes using target gas tag compositions, generating a set of random gene pools with L nodes, applying a Hopfield network for computing on energy, or cost, for each of the L gene pools and using selected constraints to establish minimum energy states to identify optimal gas tag nodes with each energy compared to a convergence threshold and then upon identifying the gas tag node continuing this procedure until establishing the next gas tag node until all remaining n nodes have been established.

  3. HaloTag Technology: A Versatile Platform for Biomedical Applications

    PubMed Central

    2015-01-01

    Exploration of protein function and interaction is critical for discovering links among genomics, proteomics, and disease state; yet, the immense complexity of proteomics found in biological systems currently limits our investigational capacity. Although affinity and autofluorescent tags are widely employed for protein analysis, these methods have been met with limited success because they lack specificity and require multiple fusion tags and genetic constructs. As an alternative approach, the innovative HaloTag protein fusion platform allows protein function and interaction to be comprehensively analyzed using a single genetic construct with multiple capabilities. This is accomplished using a simplified process, in which a variable HaloTag ligand binds rapidly to the HaloTag protein (usually linked to the protein of interest) with high affinity and specificity. In this review, we examine all current applications of the HaloTag technology platform for biomedical applications, such as the study of protein isolation and purification, protein function, protein–protein and protein–DNA interactions, biological assays, in vitro cellular imaging, and in vivo molecular imaging. In addition, novel uses of the HaloTag platform are briefly discussed along with potential future applications. PMID:25974629

  4. Evolving effective behaviours to interact with tag-based populations

    NASA Astrophysics Data System (ADS)

    Yucel, Osman; Crawford, Chad; Sen, Sandip

    2015-07-01

    Tags and other characteristics, externally perceptible features that are consistent among groups of animals or humans, can be used by others to determine appropriate response strategies in societies. This usage of tags can be extended to artificial environments, where agents can significantly reduce cognitive effort spent on appropriate strategy choice and behaviour selection by reusing strategies for interacting with new partners based on their tags. Strategy selection mechanisms developed based on this idea have successfully evolved stable cooperation in games such as the Prisoner's Dilemma game but relies upon payoff sharing and matching methods that limit the applicability of the tag framework. Our goal is to develop a general classification and behaviour selection approach based on the tag framework. We propose and evaluate alternative tag matching and adaptation schemes for a new, incoming individual to select appropriate behaviour against any population member of an existing, stable society. Our proposed approach allows agents to evolve both the optimal tag for the environment as well as appropriate strategies for existing agent groups. We show that these mechanisms will allow for robust selection of optimal strategies by agents entering a stable society and analyse the various environments where this approach is effective.

  5. Earth observation taken during STS-102

    NASA Image and Video Library

    2001-04-03

    STS102-331-012 (8-21 March 2001) --- The STS-102 crew members used a 35mm camera on the flight deck of the Space Shuttle Discovery to record this image of several meandering distributary channels of the Orinoco River draining northward into the south side of the Gulf of Paria in eastern Venezuela. According to NASA scientists studying the STS-102 collection, these sediment-laden channels carry a tremendous quantity of fluvial material that constantly changes the size and shapes of the shoreline and adjacent islands. An assortment of mud flats and sand bars, seen here as lighter colored features in the water, are affected both by stream flow and tidal forces. The extensive dark landscape identifies the flat, swampy coastal plains of northeast Venezuela that is covered by dense stands of vegetation.

  6. Evidence that coded-wire-tagging procedures can enhance transmission of Renibacterium salmoninarum in chinook salmon

    USGS Publications Warehouse

    Elliott, D.G.; Pascho, R.J.

    2001-01-01

    Binary coded wire tags (CWTs) are used extensively for identification and management of anadromous salmonid populations. A study of bacterial kidney disease (BKD) in two brood year groups of hatchery-reared spring chinook salmon Oncorhynchus tshawytscha provided strong evidence that horizontal transmission of Renibacterium salmoninarum, the causative agent of BKD, might be enhanced by CWT-marking procedures. About 4 months after CWTs were implanted in the snouts of juvenile fish, 14-16 different tissues were sampled from each of 60 fish per brood year group for histological analysis. Of the fish that were positive for R. salmoninarum by histological examination, 41% (7 of 17) of the 1988 brood year fish and 24% (10 of 42) of the 1989 brood year fish had BKD lesions confined to the head near the site of tag implantation. These lesions often resulted in the destruction of tissues of one or both olfactory organs. No focal snout infections were observed in fish that had not been marked with CWTs. Further data obtained from tissue analyses by use of an enzyme-linked immunosorbent assay and a fluorescent antibody test for detection of R. salmoninarum supported the hypothesis that infections of R. salmoninarum can be initiated in the snout tissues of CWT-marked fish and then spread to other organs. The tagging procedures might promote transmission of the pathogen among fish via contaminated tagging needles, by facilitating the entry of pathogens through the injection wound, or both. Limited evidence from this study suggested that implantation of passive integrated transponder tags in the peritoneal cavities of fish might also promote the transmission of R. salmoninarum or exacerbate existing infections. The results indicated a need for strict sanitary procedures during the tagging of fish in populations positive for R. salmoninarum to reduce the probability of enhanced horizontal transmission of the pathogen.

  7. A Hybrid Probabilistic Model for Unified Collaborative and Content-Based Image Tagging.

    PubMed

    Zhou, Ning; Cheung, William K; Qiu, Guoping; Xue, Xiangyang

    2011-07-01

    The increasing availability of large quantities of user contributed images with labels has provided opportunities to develop automatic tools to tag images to facilitate image search and retrieval. In this paper, we present a novel hybrid probabilistic model (HPM) which integrates low-level image features and high-level user provided tags to automatically tag images. For images without any tags, HPM predicts new tags based solely on the low-level image features. For images with user provided tags, HPM jointly exploits both the image features and the tags in a unified probabilistic framework to recommend additional tags to label the images. The HPM framework makes use of the tag-image association matrix (TIAM). However, since the number of images is usually very large and user-provided tags are diverse, TIAM is very sparse, thus making it difficult to reliably estimate tag-to-tag co-occurrence probabilities. We developed a collaborative filtering method based on nonnegative matrix factorization (NMF) for tackling this data sparsity issue. Also, an L1 norm kernel method is used to estimate the correlations between image features and semantic concepts. The effectiveness of the proposed approach has been evaluated using three databases containing 5,000 images with 371 tags, 31,695 images with 5,587 tags, and 269,648 images with 5,018 tags, respectively.

  8. Tri-Clustered Tensor Completion for Social-Aware Image Tag Refinement.

    PubMed

    Tang, Jinhui; Shu, Xiangbo; Qi, Guo-Jun; Li, Zechao; Wang, Meng; Yan, Shuicheng; Jain, Ramesh

    2017-08-01

    Social image tag refinement, which aims to improve tag quality by automatically completing the missing tags and rectifying the noise-corrupted ones, is an essential component for social image search. Conventional approaches mainly focus on exploring the visual and tag information, without considering the user information, which often reveals important hints on the (in)correct tags of social images. Towards this end, we propose a novel tri-clustered tensor completion framework to collaboratively explore these three kinds of information to improve the performance of social image tag refinement. Specifically, the inter-relations among users, images and tags are modeled by a tensor, and the intra-relations between users, images and tags are explored by three regularizations respectively. To address the challenges of the super-sparse and large-scale tensor factorization that demands expensive computing and memory cost, we propose a novel tri-clustering method to divide the tensor into a certain number of sub-tensors by simultaneously clustering users, images and tags into a bunch of tri-clusters. And then we investigate two strategies to complete these sub-tensors by considering (in)dependence between the sub-tensors. Experimental results on a real-world social image database demonstrate the superiority of the proposed method compared with the state-of-the-art methods.

  9. Rapid large-scale purification of myofilament proteins using a cleavable His6-tag

    PubMed Central

    Zhang, Mengjie; Martin, Jody L.; Kumar, Mohit; de Tombe, Pieter P.

    2015-01-01

    With the advent of high-throughput DNA sequencing, the number of identified cardiomyopathy-causing mutations has increased tremendously. As the majority of these mutations affect myofilament proteins, there is a need to understand their functional consequence on contraction. Permeabilized myofilament preparations coupled with protein exchange protocols are a common method for examining into contractile mechanics. However, producing large quantities of myofilament proteins can be time consuming and requires different approaches for each protein of interest. In the present study, we describe a unified automated method to produce troponin C, troponin T, and troponin I as well as myosin light chain 2 fused to a His6-tag followed by a tobacco etch virus (TEV) protease site. TEV protease has the advantage of a relaxed P1′ cleavage site specificity, allowing for no residues left after proteolysis and preservation of the native sequence of the protein of interest. After expression in Esherichia coli, cells were lysed by sonication in imidazole-containing buffer. The His6-tagged protein was then purified using a HisTrap nickel metal affinity column, and the His6-tag was removed by His6-TEV protease digestion for 4 h at 30°C. The protease was then removed using a HisTrap column, and complex assembly was performed via column-assisted sequential desalting. This mostly automated method allows for the purification of protein in 1 day and can be adapted to most soluble proteins. It has the advantage of greatly increasing yield while reducing the time and cost of purification. Therefore, production and purification of mutant proteins can be accelerated and functional data collected in a faster, less expensive manner. PMID:26386113

  10. STS-119 Launch

    NASA Image and Video Library

    2009-03-15

    STS119-S-023 (15 March 2009) --- The Space Shuttle Discovery and its seven-member STS-119 crew head toward Earth orbit and a scheduled link-up with the International Space Station. Liftoff was on time at 7:43 p.m. (EDT) on March 15, 2009 from launch pad 39A at NASA’s Kennedy Space Center. Onboard are astronauts Lee Archambault, commander; Tony Antonelli, pilot; Joseph Acaba, Steve Swanson, Richard Arnold, John Phillips and Japan Aerospace Exploration Agency’s Koichi Wakata, all mission specialists. Wakata will join Expedition 18 in progress to serve as a flight engineer aboard the space station. Discovery will deliver the final pair of power-generating solar array wings and the S6 truss segment. Installation of S6 will signal the station's readiness to house a six-member crew for conducting increased science.

  11. STS-119 Launch

    NASA Image and Video Library

    2009-03-15

    STS119-S-020 (15 March 2009) --- The Space Shuttle Discovery and its seven-member STS-119 crew head toward Earth orbit and a scheduled link-up with the International Space Station. Liftoff was on time at 7:43 p.m. (EDT) on March 15, 2009 from launch pad 39A at NASA’s Kennedy Space Center. Onboard are astronauts Lee Archambault, commander; Tony Antonelli, pilot; Joseph Acaba, Steve Swanson, Richard Arnold, John Phillips and Japan Aerospace Exploration Agency’s Koichi Wakata, all mission specialists. Wakata will join Expedition 18 in progress to serve as a flight engineer aboard the space station. Discovery will deliver the final pair of power-generating solar array wings and the S6 truss segment. Installation of S6 will signal the station's readiness to house a six-member crew for conducting increased science.

  12. STS-119 Launch

    NASA Image and Video Library

    2009-03-15

    STS119-S-028 (15 March 2009) --- The Space Shuttle Discovery and its seven-member STS-119 crew head toward Earth orbit and a scheduled link-up with the International Space Station. Liftoff was on time at 7:43 p.m. (EDT) on March 15, 2009 from launch pad 39A at NASA’s Kennedy Space Center. Onboard are astronauts Lee Archambault, commander; Tony Antonelli, pilot; Joseph Acaba, Steve Swanson, Richard Arnold, John Phillips and Japan Aerospace Exploration Agency’s Koichi Wakata, all mission specialists. Wakata will join Expedition 18 in progress to serve as a flight engineer aboard the space station. Discovery will deliver the final pair of power-generating solar array wings and the S6 truss segment. Installation of S6 will signal the station's readiness to house a six-member crew for conducting increased science.

  13. STS-119 Launch

    NASA Image and Video Library

    2009-03-15

    STS119-S-038 (15 March 2009) --- The Space Shuttle Discovery and its seven-member STS-119 crew head toward Earth orbit and a scheduled link-up with the International Space Station. Liftoff was on time at 7:43 p.m. (EDT) on March 15, 2009 from launch pad 39A at NASA’s Kennedy Space Center. Onboard are astronauts Lee Archambault, commander; Tony Antonelli, pilot; Joseph Acaba, Steve Swanson, Richard Arnold, John Phillips and Japan Aerospace Exploration Agency’s Koichi Wakata, all mission specialists. Wakata will join Expedition 18 in progress to serve as a flight engineer aboard the space station. Discovery will deliver the final pair of power-generating solar array wings and the S6 truss segment. Installation of S6 will signal the station's readiness to house a six-member crew for conducting increased science.

  14. STS-119 Launch

    NASA Image and Video Library

    2009-03-15

    STS119-S-033 (15 March 2009) --- The Space Shuttle Discovery and its seven-member STS-119 crew head toward Earth orbit and a scheduled link-up with the International Space Station. Liftoff was on time at 7:43 p.m. (EDT) on March 15, 2009 from launch pad 39A at NASA’s Kennedy Space Center. Onboard are astronauts Lee Archambault, commander; Tony Antonelli, pilot; Joseph Acaba, Steve Swanson, Richard Arnold, John Phillips and Japan Aerospace Exploration Agency’s Koichi Wakata, all mission specialists. Wakata will join Expedition 18 in progress to serve as a flight engineer aboard the space station. Discovery will deliver the final pair of power-generating solar array wings and the S6 truss segment. Installation of S6 will signal the station's readiness to house a six-member crew for conducting increased science.

  15. STS-119 Launch

    NASA Image and Video Library

    2009-03-15

    STS119-S-035 (15 March 2009) --- The Space Shuttle Discovery and its seven-member STS-119 crew head toward Earth orbit and a scheduled link-up with the International Space Station. Liftoff was on time at 7:43 p.m. (EDT) on March 15, 2009 from launch pad 39A at NASA’s Kennedy Space Center. Onboard are astronauts Lee Archambault, commander; Tony Antonelli, pilot; Joseph Acaba, Steve Swanson, Richard Arnold, John Phillips and Japan Aerospace Exploration Agency’s Koichi Wakata, all mission specialists. Wakata will join Expedition 18 in progress to serve as a flight engineer aboard the space station. Discovery will deliver the final pair of power-generating solar array wings and the S6 truss segment. Installation of S6 will signal the station's readiness to house a six-member crew for conducting increased science.

  16. STS-119 Launch

    NASA Image and Video Library

    2009-03-15

    STS119-S-018 (15 March 2009) --- The Space Shuttle Discovery and its seven-member STS-119 crew head toward Earth orbit and a scheduled link-up with the International Space Station. Liftoff was on time at 7:43 p.m. (EDT) on March 15, 2009 from launch pad 39A at NASA’s Kennedy Space Center. Onboard are astronauts Lee Archambault, commander; Tony Antonelli, pilot; Joseph Acaba, Steve Swanson, Richard Arnold, John Phillips and Japan Aerospace Exploration Agency’s Koichi Wakata, all mission specialists. Wakata will join Expedition 18 in progress to serve as a flight engineer aboard the space station. Discovery will deliver the final pair of power-generating solar array wings and the S6 truss segment. Installation of S6 will signal the station's readiness to house a six-member crew for conducting increased science.

  17. STS-119 Launch

    NASA Image and Video Library

    2009-03-15

    STS119-S-032 (15 March 2009) --- The Space Shuttle Discovery and its seven-member STS-119 crew head toward Earth orbit and a scheduled link-up with the International Space Station. Liftoff was on time at 7:43 p.m. (EDT) on March 15, 2009 from launch pad 39A at NASA’s Kennedy Space Center. Onboard are astronauts Lee Archambault, commander; Tony Antonelli, pilot; Joseph Acaba, Steve Swanson, Richard Arnold, John Phillips and Japan Aerospace Exploration Agency’s Koichi Wakata, all mission specialists. Wakata will join Expedition 18 in progress to serve as a flight engineer aboard the space station. Discovery will deliver the final pair of power-generating solar array wings and the S6 truss segment. Installation of S6 will signal the station's readiness to house a six-member crew for conducting increased science.

  18. STS-119 Launch

    NASA Image and Video Library

    2009-03-15

    STS119-S-037 (15 March 2009) --- The Space Shuttle Discovery and its seven-member STS-119 crew head toward Earth orbit and a scheduled link-up with the International Space Station. Liftoff was on time at 7:43 p.m. (EDT) on March 15, 2009 from launch pad 39A at NASA’s Kennedy Space Center. Onboard are astronauts Lee Archambault, commander; Tony Antonelli, pilot; Joseph Acaba, Steve Swanson, Richard Arnold, John Phillips and Japan Aerospace Exploration Agency’s Koichi Wakata, all mission specialists. Wakata will join Expedition 18 in progress to serve as a flight engineer aboard the space station. Discovery will deliver the final pair of power-generating solar array wings and the S6 truss segment. Installation of S6 will signal the station's readiness to house a six-member crew for conducting increased science.

  19. STS-119 Launch

    NASA Image and Video Library

    2009-03-15

    STS119-S-019 (15 March 2009) --- The Space Shuttle Discovery and its seven-member STS-119 crew head toward Earth orbit and a scheduled link-up with the International Space Station. Liftoff was on time at 7:43 p.m. (EDT) on March 15, 2009 from launch pad 39A at NASA’s Kennedy Space Center. Onboard are astronauts Lee Archambault, commander; Tony Antonelli, pilot; Joseph Acaba, Steve Swanson, Richard Arnold, John Phillips and Japan Aerospace Exploration Agency’s Koichi Wakata, all mission specialists. Wakata will join Expedition 18 in progress to serve as a flight engineer aboard the space station. Discovery will deliver the final pair of power-generating solar array wings and the S6 truss segment. Installation of S6 will signal the station's readiness to house a six-member crew for conducting increased science.

  20. STS-119 Launch

    NASA Image and Video Library

    2009-03-15

    STS119-S-034 (15 March 2009) --- The Space Shuttle Discovery and its seven-member STS-119 crew head toward Earth orbit and a scheduled link-up with the International Space Station. Liftoff was on time at 7:43 p.m. (EDT) on March 15, 2009 from launch pad 39A at NASA’s Kennedy Space Center. Onboard are astronauts Lee Archambault, commander; Tony Antonelli, pilot; Joseph Acaba, Steve Swanson, Richard Arnold, John Phillips and Japan Aerospace Exploration Agency’s Koichi Wakata, all mission specialists. Wakata will join Expedition 18 in progress to serve as a flight engineer aboard the space station. Discovery will deliver the final pair of power-generating solar array wings and the S6 truss segment. Installation of S6 will signal the station's readiness to house a six-member crew for conducting increased science.