Giant larvaceans: biologically equivalent flapping flexible foils exhibit bending modes that enhance fluid transport

NASA Astrophysics Data System (ADS)

Katija, Kakani; Sherman, Alana; Robison, Bruce

2016-11-01

The midwater region of the ocean (below the euphotic zone and above the benthos) is one of the largest ecosystems on our planet, yet remains one of the least explored. Little-known marine organisms that inhabit midwater have developed life strategies that contribute to their evolutionary success, and may inspire engineering solutions for societally relevant challenges. A group of midwater organisms, known as giant larvaceans (genus Bathochordaeus), beat their tails to drive food and particle-laden water through complex, mucus filtering structures to feed. Giant larvaceans, whose motion and kinematics resemble flapping flexible foils, range in size from 1 to 10 cm in length, and can be found between the surface and 400 m. Using remotely-operated vehicles and DeepPIV, an instrument that enables in situ particle image velocimetry (PIV) measurements, the filtration rates and kinematics of giant larvaceans were investigated. These measurements yielded filtration rates for giant larvaceans as high as 80 L/hr, which exceeds expected filtration rates by a factor of 2 when compared with other larvacean groups. Comparing tail kinematics between Bathochordeaus and smaller larvaceans reveals differences in tail bending modes, where a hinge is present throughout the tail beat in giant larvaceans. Using laboratory PIV measurements with swimming animals and soft-bodied mechanical mimics, we reveal how these differences in tail kinematics can lead to enhanced fluid transport. This work has been supported by the Packard Foundation.

BIOMECHANICS. Why the seahorse tail is square.

PubMed

Porter, Michael M; Adriaens, Dominique; Hatton, Ross L; Meyers, Marc A; McKittrick, Joanna

2015-07-03

Whereas the predominant shapes of most animal tails are cylindrical, seahorse tails are square prisms. Seahorses use their tails as flexible grasping appendages, in spite of a rigid bony armor that fully encases their bodies. We explore the mechanics of two three-dimensional-printed models that mimic either the natural (square prism) or hypothetical (cylindrical) architecture of a seahorse tail to uncover whether or not the square geometry provides any functional advantages. Our results show that the square prism is more resilient when crushed and provides a mechanism for preserving articulatory organization upon extensive bending and twisting, as compared with its cylindrical counterpart. Thus, the square architecture is better than the circular one in the context of two integrated functions: grasping ability and crushing resistance. Copyright © 2015, American Association for the Advancement of Science.

Calculations of critical micelle concentration by dissipative particle dynamics simulations: the role of chain rigidity.

PubMed

Lee, Ming-Tsung; Vishnyakov, Aleksey; Neimark, Alexander V

2013-09-05

Micelle formation in surfactant solutions is a self-assembly process governed by complex interplay of solvent-mediated interactions between hydrophilic and hydrophobic groups, which are commonly called heads and tails. However, the head-tail repulsion is not the only factor affecting the micelle formation. For the first time, we present a systematic study of the effect of chain rigidity on critical micelle concentration and micelle size, which is performed with the dissipative particle dynamics simulation method. Rigidity of the coarse-grained surfactant molecule was controlled by the harmonic bonds set between the second-neighbor beads. Compared to flexible molecules with the nearest-neighbor bonds being the only type of bonded interactions, rigid molecules exhibited a lower critical micelle concentration and formed larger and better-defined micelles. By varying the strength of head-tail repulsion and the chain rigidity, we constructed two-dimensional diagrams presenting how the critical micelle concentration and aggregation number depend on these parameters. We found that the solutions of flexible and rigid molecules that exhibited approximately the same critical micelle concentration could differ substantially in the micelle size and shape depending on the chain rigidity. With the increase of surfactant concentration, primary micelles of more rigid molecules were found less keen to agglomeration and formation of nonspherical aggregates characteristic of flexible molecules.

Flapping foil power generator performance enhanced with a spring-connected tail

NASA Astrophysics Data System (ADS)

Liu, Zhengliang; Tian, Fang-Bao; Young, John; Lai, Joseph C. S.

2017-12-01

The flexibility effects on the performance of a flapping foil power generator are numerically studied by using the immersed boundary-lattice Boltzmann method at a Reynolds number of 1100. The flapping foil system consists of a rigid NACA0015 foil undergoing harmonic pitch and plunge motions and a passively actuated flat plate pinned to the trailing edge of the rigid foil. The flexibility is modeled by a torsional spring model at the conjuncture of the rigid foil and the tail. Here, a parametric study on mass density and natural frequency is conducted under the optimum kinematic condition of the rigid system identified from the literature and numerical simulations made for reduced frequency f* = 0.04-0.24 and pitch amplitude θ0 = 40°-90°. Four typical cases are discussed in detail by considering time histories of hydrodynamic loads and tail deformations under the optimal and non-optimal kinematic conditions. Results show that under the rigid-system optimal kinematic condition, a tail with appropriate mass density (μ = 0.60) and resonant frequency ( fr*=1.18 ) can improve the maximum efficiency by 7.24% accompanied by an increase of 6.63% in power compared to those of a rigid foil with a rigid tail. This is because the deflection of the tail reduces the low pressure region on the pressure surface (i.e., the lower surface during the upstroke or the upper surface during the downstroke) caused by the leading edge vortex after the stroke reversal, resulting in a higher efficiency. At high flapping frequencies, a spring-connected tail ( fr*=0.13 ) eliminates the large spike in the moment observed in high stiffness cases, reducing the power required for the pitch motion, resulting in 117% improvement in efficiency over that with a rigid tail at a reduced frequency of 0.24.

H3 Histone Tail Conformation within the Nucleosome and the Impact of K14 Acetylation Studied Using Enhanced Sampling Simulation

PubMed Central

Ikebe, Jinzen; Sakuraba, Shun; Kono, Hidetoshi

2016-01-01

Acetylation of lysine residues in histone tails is associated with gene transcription. Because histone tails are structurally flexible and intrinsically disordered, it is difficult to experimentally determine the tail conformations and the impact of acetylation. In this work, we performed simulations to sample H3 tail conformations with and without acetylation. The results show that irrespective of the presence or absence of the acetylation, the H3 tail remains in contact with the DNA and assumes an α-helix structure in some regions. Acetylation slightly weakened the interaction between the tail and DNA and enhanced α-helix formation, resulting in a more compact tail conformation. We inferred that this compaction induces unwrapping and exposure of the linker DNA, enabling DNA-binding proteins (e.g., transcription factors) to bind to their target sequences. In addition, our simulation also showed that acetylated lysine was more often exposed to the solvent, which is consistent with the fact that acetylation functions as a post-translational modification recognition site marker. PMID:26967163

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ye, Qiaozhen; Krug, Robert M.; Tao, Yizhi Jane

Influenza A viruses pose a serious threat to world public health, particularly the currently circulating avian H5N1 viruses. The influenza viral nucleoprotein forms the protein scaffold of the helical genomic ribonucleoprotein complexes, and has a critical role in viral RNA replication. Here we report a 3.2 Angstrom crystal structure of this nucleoprotein, the overall shape of which resembles a crescent with a head and a body domain, with a protein fold different compared with that of the rhabdovirus nucleoprotein. Oligomerization of the influenza virus nucleoprotein is mediated by a flexible tail loop that is inserted inside a neighboring molecule. Thismore » flexibility in the tail loop enables the nucleoprotein to form loose polymers as well as rigid helices, both of which are important for nucleoprotein functions. Single residue mutations in the tail loop result in the complete loss of nucleoprotein oligomerization. An RNA-binding groove, which is found between the head and body domains at the exterior of the nucleoprotein oligomer, is lined with highly conserved basic residues widely distributed in the primary sequence. The nucleoprotein structure shows that only one of two proposed nuclear localization signals are accessible, and suggests that the body domain of nucleoprotein contains the binding site for the viral polymerase. Our results identify the tail loop binding pocket as a potential target for antiviral development.« less

Unscrambling the effect of C-terminal tail deletion on the stability of a cold-adapted, organic solvent stable lipase from Staphylococcus epidermidis AT2.

PubMed

Kamarudin, Nor Hafizah Ahmad; Rahman, Raja Noor Zaliha Raja Abd; Ali, Mohd Shukuri Mohamad; Leow, Thean Chor; Basri, Mahiran; Salleh, Abu Bakar

2014-08-01

Terminal moieties of most proteins are long known to be disordered and flexible. To unravel the functional role of these regions on the structural stability and biochemical properties of AT2 lipase, four C-terminal end residues, (Ile-Thr-Arg-Lys) which formed a flexible, short tail-like random-coil segment were targeted for mutation. Swapping of the tail-like region had resulted in an improved crystallizability and anti-aggregation property along with a slight shift of the thermostability profile. The lipolytic activity of mutant (M386) retained by 43 % compared to its wild-type with 18 % of the remaining activity at 45 °C. In silico analysis conducted at 25 and 45 °C was found to be in accordance to the experimental findings in which the RMSD values of M386 were more stable throughout the total trajectory in comparison to its wild-type. Terminal moieties were also observed to exhibit large movement and flexibility as denoted by high RMSF values at both dynamics. Variation in organic solvent stability property was detected in M386 where the lipolytic activity was stimulated in the presence of 25 % (v/v) of DMSO, isopropanol, and diethyl ether. This may be worth due to changes in the surface charge residues at the mutation point which probably involve in protein-solvent interaction.

Aerodynamics of a Flapping Airfoil with a Flexible Tail

NASA Astrophysics Data System (ADS)

Lai, Alan Kai San

This dissertation presents computational solutions to an airfoil in a oscillatory heaving motion with a aeroelastically flexible tail attachment. An unsteady potential flow solver is coupled to a structural solver to obtain the aeroelastic flow solution over an inviscid fluid to investigate the propulsive performance of such a configuration. The simulation is then extended to a two-dimensional viscous solver by coupling NASA's CFL3D solver to the structural solver to study how the flow is altered by the presence of viscosity. Finally, additional simulations are done in three dimensions over wings with varying aspect ratio to study the three-dimensional effects on the propulsive performance of an airfoil with an aeroelastic tail. The computation reveals that the addition of the aeroelastic trailing edge improved the thrust generated by a heaving airfoil significantly. As the frequency of the heaving motion increases, the thrust generated by the airfoil with the tail increases exponentially. In an inviscid fluid, the increase in thrust is insufficient to overcome the increase in power required to maintain the motion and as a result the overall propulsive efficiency is reduced. When the airfoil is heaving in a viscous fluid, the presence of a suction boundary layer and the appearance of leading edge vortex increase the thrust generated to such an extent that the propulsive efficiency is increased by about 3% when compared to the same airfoil with a rigid tail. The three-dimensional computations shows that the presence of the tip vorticies suppress some of the increase in thrust observed in the two-dimensional viscous computations for short span wings. For large span wings, the overall thrust enhancing capabilities of the aeroelastic tail is preserved.

Visualizing viral transport and host infection

NASA Astrophysics Data System (ADS)

Son, Kwangmin; Guasto, Jeffrey; Cubillos-Ruiz, Andres; Sullivan, Matthew; Stocker, Roman; MIT Team

2013-11-01

A virus is a non-motile infectious agent that can only replicate inside a living host. They consist of a <100 nm diameter capsid which houses their DNA, and a <20 nm diameter tail used to inject DNA to the host, which are classified into three different morphologies by the tail type: short tail (~ 10 nm, podovirus), rigid contractile tail (~ 100 nm, myovirus), or flexible noncontractile tail (~ 300 nm, siphovirus). Combining microfluidics with epifluorescent microscopy, we studied the simultaneous diffusive transport governing the initial encounter and ultimately the infection of a non-motile cyanobacteria host (~ 1 μm prochlorococcus) and their viral (phage) counterparts in real time. This methodology allows us to quantify the virus-host encounter/adsorption dynamics and subsequently the effectiveness of various tail morphologies for viral infection. Viral transport and the role of viral morphology in host-virus interactions are critical to our understanding of both ecosystem dynamics and human health, as well as to the evolution of virus morphology.

Tadpole-like artificial micromotor

NASA Astrophysics Data System (ADS)

Liu, Limei; Liu, Mei; Su, Yajun; Dong, Yonggang; Zhou, Wei; Zhang, Lina; Zhang, Hui; Dong, Bin; Chi, Lifeng

2015-01-01

We describe a polymer-based artificial tadpole-like micromotor, which is fabricated through the electrospinning technique. By incorporating functional materials onto its surface or within its body, the resulting tadpole-like micromotor can not only move autonomously in an aqueous solution with a flexible tail, but also exhibit thermo- and magnetic responsive properties.We describe a polymer-based artificial tadpole-like micromotor, which is fabricated through the electrospinning technique. By incorporating functional materials onto its surface or within its body, the resulting tadpole-like micromotor can not only move autonomously in an aqueous solution with a flexible tail, but also exhibit thermo- and magnetic responsive properties. Electronic supplementary information (ESI) available: Experimental section, Fig. S1-S3 and Video S1-S4. See DOI: 10.1039/c4nr06621a

The measles virus N(TAIL)-XD complex: an illustrative example of fuzziness.

PubMed

Longhi, Sonia

2012-01-01

In this chapter, I focus on the biochemical and structural characterization of the complex between the intrinsically disordered C-terminal domain of the measles virus nucleoprotein (N(TAIL)) and the C-terminal X domain (XD) of the viral phosphoprotein (P). I summarize the main experimental data available so far pointing out the prevalently disordered nature of N(TAIL) even after complex formation and the role of the flexible C-terminal appendage in the binding reaction. I finally discuss the possible functional role of these residual disordered regions within the complex in terms of their ability to capture other regulatory, binding partners.

Postsacral vertebral morphology in relation to tail length among primates and other mammals.

PubMed

Russo, Gabrielle A

2015-02-01

Tail reduction/loss independently evolved in a number of mammalian lineages, including hominoid primates. One prerequisite to appropriately contextualizing its occurrence and understanding its significance is the ability to track evolutionary changes in tail length throughout the fossil record. However, to date, the bony correlates of tail length variation among living taxa have not been comprehensively examined. This study quantifies postsacral vertebral morphology among living primates and other mammals known to differ in relative tail length (RTL). Linear and angular measurements with known biomechanical significance were collected on the first, mid-, and transition proximal postsacral vertebrae, and their relationship with RTL was assessed using phylogenetic generalized least-squares regression methods. Compared to shorter-tailed primates, longer-tailed primates possess a greater number of postsacral vertebral features associated with increased proximal tail flexibility (e.g., craniocaudally longer vertebral bodies), increased intervertebral body joint range of motion (e.g., more circularly shaped cranial articular surfaces), and increased leverage of tail musculature (e.g., longer spinous processes). These observations are corroborated by the comparative mammalian sample, which shows that distantly related short-tailed (e.g., Phascolarctos, Lynx) and long-tailed (e.g., Dendrolagus, Acinonyx) nonprimate mammals morphologically converge with short-tailed (e.g., Macaca tonkeana) and long-tailed (e.g., Macaca fascicularis) primates, respectively. Multivariate models demonstrate that the variables examined account for 70% (all mammals) to 94% (only primates) of the variance in RTL. Results of this study may be used to infer the tail lengths of extinct primates and other mammals, thereby improving our understanding about the evolution of tail reduction/loss. © 2014 Wiley Periodicals, Inc.

Adaptive Suction and Blowing for Twin-Tail Buffet Control

NASA Technical Reports Server (NTRS)

Kandil, Osama A.; Yang, Zhi

1999-01-01

Adaptive active flow control for twin-tail buffet alleviation is investigated. The concept behind this technique is to place control ports on the tail outer and inner surfaces with flow suction or blowing applied through these ports in order to minimize the pressure difference across the tail. The suction or blowing volume flow rate from each port is proportional to the pressure difference across the tail at this location. A parametric study of the effects of the number and location of these ports on the buffet response is carried out. The computational model consists of a sharp-edged delta wing of aspect ratio one and swept-back flexible twin tail with taper ratio of 0.23. This complex multidisciplinary problem is solved sequentially using three sets of equations for the fluid flow, aeroelastic response and grid deformation, using a dynamic multi-block grid structure. The computational model is pitched at 30 deg angle of attack. The freestream Mach number and Reynolds number are 0.3 and 1.25 million, respectively. The model is investigated for the inboard position of the twin tails, which corresponds to a separation distance between the twin tails of 33% of the wing span. Comparison of the time history and power spectral density responses of the tails for various distributions of the control ports are presented and discussed.

Highly deformable bones: unusual deformation mechanisms of seahorse armor.

PubMed

Porter, Michael M; Novitskaya, Ekaterina; Castro-Ceseña, Ana Bertha; Meyers, Marc A; McKittrick, Joanna

2013-06-01

Multifunctional materials and devices found in nature serve as inspiration for advanced synthetic materials, structures and robotics. Here, we elucidate the architecture and unusual deformation mechanisms of seahorse tails that provide prehension as well as protection against predators. The seahorse tail is composed of subdermal bony plates arranged in articulating ring-like segments that overlap for controlled ventral bending and twisting. The bony plates are highly deformable materials designed to slide past one another and buckle when compressed. This complex plate and segment motion, along with the unique hardness distribution and structural hierarchy of each plate, provide seahorses with joint flexibility while shielding them against impact and crushing. Mimicking seahorse armor may lead to novel bio-inspired technologies, such as flexible armor, fracture-resistant structures or prehensile robotics. Copyright © 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Flow Behavior Around a Fast-Starting Robotic Fish

NASA Astrophysics Data System (ADS)

Ma, Ganzhong; Currier, Todd; Modarres-Sadeghi, Yahya

2017-11-01

A robotic fish is used to study the flow behavior around the body of a fast-starting fish as it experiences a fast-start. The robotic fish is designed and built emulating a Northern Pike, Esox Lucius, which can accelerate at up to 245 m/s2. In previous studies, we had focused on the flow around the tail during the fast-start, by using a tail which acted flexibly in the preparatory stage and rigidly in the propulsive stage. We have extended that study by including the fish body in the experimental setup, where the body can bend into a C-shape, so that the influence of the body motion on the resulting flow around the structure can be understood as well. In the tests, the fish can rotate about a vertical axis, where a multi-axis force sensor measures flow forces acting on the body. Synchronized with the force measurement, flow visualizations using bubble image velocimetry are conducted, and the observed shed vortices are related to the peak forces observed during the maneuver.

Molecular dynamics simulation on HP1 protein binding by histone H3 tail methylation and phosphorylation

NASA Astrophysics Data System (ADS)

Jiang, Yan-Ke; Zou, Jian-Wei; Wu, Yu-Qian; Zhang, Na; Yu, Qing-Sen; Jiang, Yong-Jun

Trimethylation of histone H3 lysine 9 is important for recruiting heterochromatin protein 1 (HP1) to discrete regions of the genome, thereby regulating gene expression, chromatin packaging, and heterochromatin formation. Phosphorylation of histone H3 has been linked with mitotic chromatin condensation. During mitosis in vivo, H3 lysine 9 methylation and serine 10 phosphorylation can occur concomitantly on the same histone tail, whereas the influence of phosphorylation to trimethylation H3 tail recruiting HP1 remains controversial. In this work, molecular dynamics simulation of HP1 complexed with both trimethylated and phosphorylated H3 tail were performed and compared with the results from the previous methylated H3-HP1 trajectory. It is clear from the 10-ns dynamics simulation that two adjacent posttranslational modifications directly increase the flexibility of the H3 tail and weaken HP1 binding to chromatin. A combinatorial readout of two adjacent posttranslational modifications-a stable methylation and a dynamic phosphorylation mark-establish a regulatory mechanism of protein-protein interactions.

Effectiveness of Flow Control for Alleviation of Twin-Tail Buffet

NASA Technical Reports Server (NTRS)

Sheta, Essam F.; Kandil, Osama A.; Yang, Zhi

1998-01-01

Effectiveness of active flow control for twin- tail buffet alleviation is investigated. Tangen- tial leading-edge blowing (TLEB) and flow suction along the vortex cores (FSVC) of the lead- ing edges of the delta wing are used to delay the vortex breakdown flow upstream of the twin tail. The combined effect of the TLEB and FSVC is also investigated. A parametric study of the effects of the spanwise position of the suction tubes and volumetric suction flow rate on the twin-tail buffet response are also investigated. The TLEB moves the path of leading-edge vortices laterally towards the twin tail, which increases the aero- dynamic damping on the tails. The FSVC effectively delays the breakdown location at high angles of attack. The computational model consists of a sharp-edged delta wing of aspect ratio one and swept-back flexible twin tail with taper ratio of 0.23. This complex multidisciplinary problem is solved sequentially using three sets of equations for the fluid flow, aeroelastic response and grid deformation, on a dynamic multi-block grid structure. The computational model is pitched at 30 deg. angle of attack. The freestream Mach number and Reynolds number are 0.3 and 1.25 million, respectively. The model is investigated for the inboard position of the twin tails, which corresponds to a separation distance between the twin tails of 33% of the wing span.

The asymptotic structure of a slender coiling fluid thread

NASA Astrophysics Data System (ADS)

Blount, Maurice; Lister, John

2010-11-01

The buckling of a viscous fluid thread as it falls through air onto a stationary surface is a well-known breakfast-time phenomenon which exhibits a rich variety of dynamical regimes [1]. Since the bending resistance of a slender thread is small, bending motion is largely confined to a short region of coiling near the surface. If the height of fall is large enough, then the thread above the coiling region forms a `tail' that falls nearly vertically under gravity but is deflected slightly due to forces exerted on it by the coil. Although it is possible to use force balances in the coil to estimate scalings for the coiling frequency, we analyse the solution structure of the entire thread in the asymptotic limit of a very slender thread and thereby include the dynamic interaction between the coil and the tail. Quantitative predictions of the coiling frequency are obtained which demonstrate the existence of leading-order corrections to scalings previously derived. In particular, we show that in the regime where the deflection of the tail is governed by a balance between centrifugal acceleration, hoop stress and gravity, the tail behaves as a flexible circular pendulum that is forced by bending stress exerted by the coil. The amplitude of the response is calculated and the previously observed resonance when the coiling frequency coincides with one of the eigenfrequencies of a free flexible pendulum is thereby explained. [1] N.M. Ribe et al., J. Fluid Mech. 555, 275-297.

Parallel basal ganglia circuits for voluntary and automatic behaviour to reach rewards

PubMed Central

Hikosaka, Okihide

2015-01-01

The basal ganglia control body movements, value processing and decision-making. Many studies have shown that the inputs and outputs of each basal ganglia structure are topographically organized, which suggests that the basal ganglia consist of separate circuits that serve distinct functions. A notable example is the circuits that originate from the rostral (head) and caudal (tail) regions of the caudate nucleus, both of which target the superior colliculus. These two caudate regions encode the reward values of visual objects differently: flexible (short-term) values by the caudate head and stable (long-term) values by the caudate tail. These value signals in the caudate guide the orienting of gaze differently: voluntary saccades by the caudate head circuit and automatic saccades by the caudate tail circuit. Moreover, separate groups of dopamine neurons innervate the caudate head and tail and may selectively guide the flexible and stable learning/memory in the caudate regions. Studies focusing on manual handling of objects also suggest that rostrocaudally separated circuits in the basal ganglia control the action differently. These results suggest that the basal ganglia contain parallel circuits for two steps of goal-directed behaviour: finding valuable objects and manipulating the valuable objects. These parallel circuits may underlie voluntary behaviour and automatic skills, enabling animals (including humans) to adapt to both volatile and stable environments. This understanding of the functions and mechanisms of the basal ganglia parallel circuits may inform the differential diagnosis and treatment of basal ganglia disorders. PMID:25981958

The X-ray Crystal Structure of the Phage Tail Terminator Protein Reveals the Biologically Relevant Hexameric Rang Structure and Demonstrates a Conserved mechanism of Tail Termination among Divrse Long Tailed Phages

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pell, L.; Liu, A; Edmonds, L

The tail terminator protein (TrP) plays an essential role in phage tail assembly by capping the rapidly polymerizing tail once it has reached its requisite length and serving as the interaction surface for phage heads. Here, we present the 2.7-A crystal structure of a hexameric ring of gpU, the TrP of phage ?. Using sequence alignment analysis and site-directed mutagenesis, we have shown that this multimeric structure is biologically relevant and we have delineated its functional surfaces. Comparison of the hexameric crystal structure with the solution structure of gpU that we previously solved using NMR spectroscopy shows large structural changesmore » occurring upon multimerization and suggests a mechanism that allows gpU to remain monomeric at high concentrations on its own, yet polymerize readily upon contact with an assembled tail tube. The gpU hexamer displays several flexible loops that play key roles in head and tail binding, implying a role for disorder-to-order transitions in controlling assembly as has been observed with other ? morphogenetic proteins. Finally, we have found that the hexameric structure of gpU is very similar to the structure of a putative TrP from a contractile phage tail even though it displays no detectable sequence similarity. This finding coupled with further bioinformatic investigations has led us to conclude that the TrPs of non-contractile-tailed phages, such as ?, are evolutionarily related to those of contractile-tailed phages, such as P2 and Mu, and that all long-tailed phages may utilize a conserved mechanism for tail termination.« less

The Tail Suspension Test

PubMed Central

Terrillion, Chantelle E.; Piantadosi, Sean C.; Bhat, Shambhu; Gould, Todd D.

2012-01-01

The tail-suspension test is a mouse behavioral test useful in the screening of potential antidepressant drugs, and assessing of other manipulations that are expected to affect depression related behaviors. Mice are suspended by their tails with tape, in such a position that it cannot escape or hold on to nearby surfaces. During this test, typically six minutes in duration, the resulting escape oriented behaviors are quantified. The tail-suspension test is a valuable tool in drug discovery for high-throughput screening of prospective antidepressant compounds. Here, we describe the details required for implementation of this test with additional emphasis on potential problems that may occur and how to avoid them. We also offer a solution to the tail climbing behavior, a common problem that renders this test useless in some mouse strains, such as the widely used C57BL/6. Specifically, we prevent tail climbing behaviors by passing mouse tails through a small plastic cylinder prior to suspension. Finally, we detail how to manually score the behaviors that are manifested in this test. PMID:22315011

A new trajectory concept for exploring the earth's geomagnetic tail

NASA Technical Reports Server (NTRS)

Farquhar, R. W.; Dunham, D. W.

1981-01-01

An innovative trajectory technique for a magnetotail mapping mission is described which can control the apsidal rotation of an elliptical earth orbit and keep its apogee segment inside the tail region. The required apsidal rotation rate of approximately 1 deg/day is achieved by using the moon to carry out a prescribed sequence of gravity-assist maneuvers. Apogee distances are alternately raised and lowered by the lunar-swingby maneuvers; several categories of the 'sun-synchronous' swingby trajectories are identified. The strength and flexibility of the new trajectory concept is demonstrated by using real-world simulations showing that a large variety of trajectory shapes can be used to explore the earth's geomagnetic tail between 60 and 250 R sub E.

A feasibility study regarding the addition of a fifth control to a rotorcraft in-flight simulator

NASA Technical Reports Server (NTRS)

Turner, Simon; Andrisani, Dominick, II

1992-01-01

The addition of a large movable horizontal tail surface to the control system of a rotorcraft in-flight simulator being developed from a Sikorsky UH-60A Black Hawk Helicopter is evaluated. The capabilities of the control surface as a trim control and as an active control are explored. The helicopter dynamics are modeled using the Generic Helicopter simulation program developed by Sikorsky Aircraft. The effect of the horizontal tail on the helicopter trim envelope is examined by plotting trim maps of the aircraft attitude and controls as a function of the flight speed and horizontal tail incidence. The control power of the tail surface relative to that of the other controls is examined by comparing control derivatives extracted from the simulation program over the flight speed envelope. The horizontal tail's contribution as an active control is evaluated using an explicit model following control synthesis involving a linear model of the helicopter in steady, level flight at a flight speed of eighty knots. The horizontal tail is found to provide additional control flexibility in the longitudinal axis. As a trim control, it provides effective control of the trim pitch attitude at mid to high forward speeds. As an active control, the horizontal tail provides useful pitching moment generating capabilities at mid to high forward speeds.

The N–Terminal Tail of hERG Contains an Amphipathic α–Helix That Regulates Channel Deactivation

PubMed Central

Mobli, Mehdi; Ke, Ying; Kuchel, Philip W.; King, Glenn F.; Stock, Daniela; Vandenberg, Jamie I.

2011-01-01

The cytoplasmic N–terminal domain of the human ether–a–go–go related gene (hERG) K+ channel is critical for the slow deactivation kinetics of the channel. However, the mechanism(s) by which the N–terminal domain regulates deactivation remains to be determined. Here we show that the solution NMR structure of the N–terminal 135 residues of hERG contains a previously described Per–Arnt–Sim (PAS) domain (residues 26–135) as well as an amphipathic α–helix (residues 13–23) and an initial unstructured segment (residues 2–9). Deletion of residues 2–25, only the unstructured segment (residues 2–9) or replacement of the α–helix with a flexible linker all result in enhanced rates of deactivation. Thus, both the initial flexible segment and the α–helix are required but neither is sufficient to confer slow deactivation kinetics. Alanine scanning mutagenesis identified R5 and G6 in the initial flexible segment as critical for slow deactivation. Alanine mutants in the helical region had less dramatic phenotypes. We propose that the PAS domain is bound close to the central core of the channel and that the N–terminal α–helix ensures that the flexible tail is correctly orientated for interaction with the activation gating machinery to stabilize the open state of the channel. PMID:21249148

Varieties of charge distributions in coat proteins of ssRNA+  viruses

NASA Astrophysics Data System (ADS)

Lošdorfer Božič, Anže; Podgornik, Rudolf

2018-01-01

A major part of the interactions involved in the assembly and stability of icosahedral, positive-sense single-stranded RNA (ssRNA+) viruses is electrostatic in nature, as can be inferred from the strong pH- and salt-dependence of their assembly phase diagrams. Electrostatic interactions do not act only between the capsid coat proteins (CPs), but just as often provide a significant contribution to the interactions of the CPs with the genomic RNA, mediated to a large extent by positively charged, flexible N-terminal tails of the CPs. In this work, we provide two clear and complementary definitions of an N-terminal tail of a protein, and use them to extract the tail sequences of a large number of CPs of ssRNA+  viruses. We examine the pH-dependent interplay of charge on both tails and CPs alike, and show that—in contrast to the charge on the CPs—the net positive charge on the N-tails persists even to very basic pH values. In addition, we note a limit to the length of the wild-type genomes of those viruses which utilize positively charged tails, when compared to viruses without charged tails and similar capsid size. At the same time, we observe no clear connection between the charge on the N-tails and the genome lengths of the viruses included in our study.

Low-speed wind tunnel test results of the Canard Rotor/Wing concept

NASA Technical Reports Server (NTRS)

Bass, Steven M.; Thompson, Thomas L.; Rutherford, John W.; Swanson, Stephen

1993-01-01

The Canard Rotor/Wing (CRW), a high-speed rotorcraft concept, was tested at the National Aeronautics and Space Administration (NASA) Ames Research Center's 40- by 80-Foot Wind Tunnel in Mountain View, California. The 1/5-scale model was tested to identify certain low-speed, fixed-wing, aerodynamic characteristics of the configuration and investigate the effectiveness of two empennages, an H-Tail and a T-Tail. The paper addresses the principal test objectives and the results achieved in the wind tunnel test. These are summarized as: i) drag build-up and differences between the H-Tail and T-Tail configuration, ii) longitudinal stability of the H-Tail and T-Tail configurations in the conversion and cruise modes, iii) control derivatives for the canard and elevator in the conversion and cruise modes, iv) aerodynamic characteristics of varying the rotor/wing azimuth position, and v) canard and tail lift/trim capability for conversion conditions.

Flexible architecture of inducible morphological plasticity.

PubMed

Kishida, Osamu; Nishimura, Kinya

2006-05-01

1. Predator-induced morphological defences are produced in response to an emergent predator regime. In natural systems, prey organisms usually experience temporal shifting of the composition of the predator assemblage and of the intensity of predation risk from each predator species. Although, a repetitive morphological change in response to a sequential shift of the predator regime such as alteration of the predator species or diminution of the predation risk may be adaptive, such flexible inducible morphological defences are not ubiquitous. 2. We experimentally addressed whether a flexible inducible morphological defence is accomplished in response to serial changes in the predation regime, using a model prey species which adopt different defensive morphological phenotypes in response to different predator species. Rana pirica (Matsui) tadpoles increased body depth and tail depth against the predatory larval salamander Hynobius retardatus (Dunn); on the other hand, they only increased tail depth against the predatory larval dragonfly Aeshna nigroflava (Martin). 3. Rana pirica tadpoles with the predator-specific phenotypes were subjected to removal or exchange of the predator species. After removal of the predator species, tadpoles with each predator-specific phenotype changed their phenotype to the nondefensive basic one, suggesting that both predator-specific phenotypes are costly to maintain. After an exchange of the predator species, tadpoles with each predator-specific phenotype reciprocally, flexibly shifted their phenotype to the now more suitable predator-specific one only by modifying their body part. The partial modification can effectively reduce time and energy expenditures involved in repetitive morphological changes, and therefore suggest that the costs of the flexible morphological changes are reduced.

A magnetospheric magnetic field model with flexible current systems driven by independent physical parameters

NASA Technical Reports Server (NTRS)

Hilmer, Robert V.; Voigt, Gerd-Hannes

1995-01-01

A tilt-dependent magnetic field model of the Earth's magnetosphere with variable magnetopause standoff distance is presented. Flexible analytic representations for the ring and cross-tail currents, each composed of the elements derived from the Tsyganenko and Usmanov (1982) model, are combined with the fully shielded vacuum dipole configurations of Voigt (1981). Although the current sheet does not warp in the y-z plane, changes in the shape and position of the neutral sheet with dipole tilt are consistent with both MHD equilibrium theory and observations. In addition, there is good agreement with observed Delta B profiles and the average equatorial contours of magnetic field magnitude. While the dipole field is rigorously shielded within the defined magnetopause, the ring and cross-tails currents are not similarly confined, consequently, the model's region of validity is limited to the inner magnetosphere. The model depends on four independent external parameters. We present a simple but limited method of simulating several substorm related magnetic field changes associated with the disrupion of the near-Earth cross-tail current sheet and collapse of the midnight magnetotail field region. This feature further facilitates the generation of magnetic field configuration time sequences useful in plasma convection simulations of real magnetospheric events.

Mapping flexible protein domains at subnanometer resolution with the atomic force microscope.

PubMed

Müller, D J; Fotiadis, D; Engel, A

1998-06-23

The mapping of flexible protein domains with the atomic force microscope is reviewed. Examples discussed are the bacteriorhodopsin from Halobacterium salinarum, the head-tail-connector from phage phi29, and the hexagonally packed intermediate layer from Deinococcus radiodurans which all were recorded in physiological buffer solution. All three proteins undergo reversible structural changes that are reflected in standard deviation maps calculated from aligned topographs of individual protein complexes. Depending on the lateral resolution (up to 0.8 nm) flexible surface regions can ultimately be correlated with individual polypeptide loops. In addition, multivariate statistical classification revealed the major conformations of the protein surface.

Molecular dynamics simulations demonstrate the regulation of DNA-DNA attraction by H4 histone tail acetylations and mutations.

PubMed

Korolev, Nikolay; Yu, Hang; Lyubartsev, Alexander P; Nordenskiöld, Lars

2014-10-01

The positively charged N-terminal histone tails play a crucial role in chromatin compaction and are important modulators of DNA transcription, recombination, and repair. The detailed mechanism of the interaction of histone tails with DNA remains elusive. To model the unspecific interaction of histone tails with DNA, all-atom molecular dynamics (MD) simulations were carried out for systems of four DNA 22-mers in the presence of 20 or 16 short fragments of the H4 histone tail (variations of the 16-23 a. a. KRHRKVLR sequence, as well as the unmodified fragment a. a.13-20, GGAKRHRK). This setup with high DNA concentration, explicit presence of DNA-DNA contacts, presence of unstructured cationic peptides (histone tails) and K(+) mimics the conditions of eukaryotic chromatin. A detailed account of the DNA interactions with the histone tail fragments, K(+) and water is presented. Furthermore, DNA structure and dynamics and its interplay with the histone tail fragments binding are analysed. The charged side chains of the lysines and arginines play major roles in the tail-mediated DNA-DNA attraction by forming bridges and by coordinating to the phosphate groups and to the electronegative sites in the minor groove. Binding of all species to DNA is dynamic. The structure of the unmodified fully-charged H4 16-23 a.a. fragment KRHRKVLR is dominated by a stretched conformation. The H4 tail a. a. fragment GGAKRHRK as well as the H4 Lys16 acetylated fragment are highly flexible. The present work allows capturing typical features of the histone tail-counterion-DNA structure, interaction and dynamics. © 2014 Wiley Periodicals, Inc.

Unraveling the structural complexity in a single-stranded RNA tail: implications for efficient ligand binding in the prequeuosine riboswitch

PubMed Central

Eichhorn, Catherine D.; Feng, Jun; Suddala, Krishna C.; Walter, Nils G.; Brooks, Charles L.; Al-Hashimi, Hashim M.

2012-01-01

Single-stranded RNAs (ssRNAs) are ubiquitous RNA elements that serve diverse functional roles. Much of our understanding of ssRNA conformational behavior is limited to structures in which ssRNA directly engages in tertiary interactions or is recognized by proteins. Little is known about the structural and dynamic behavior of free ssRNAs at atomic resolution. Here, we report the collaborative application of nuclear magnetic resonance (NMR) and replica exchange molecular dynamics (REMD) simulations to characterize the 12 nt ssRNA tail derived from the prequeuosine riboswitch. NMR carbon spin relaxation data and residual dipolar coupling measurements reveal a flexible yet stacked core adopting an A-form-like conformation, with the level of order decreasing toward the terminal ends. An A-to-C mutation within the polyadenine tract alters the observed dynamics consistent with the introduction of a dynamic kink. Pre-ordering of the tail may increase the efficacy of ligand binding above that achieved by a random-coil ssRNA. The REMD simulations recapitulate important trends in the NMR data, but suggest more internal motions than inferred from the NMR analysis. Our study unmasks a previously unappreciated level of complexity in ssRNA, which we believe will also serve as an excellent model system for testing and developing computational force fields. PMID:22009676

At-sea behavior varies with lunar phase in a nocturnal pelagic seabird, the swallow-tailed gull

USGS Publications Warehouse

Cruz, Sebastian M.; Hooten, Mevin; Huyvaert, Kathryn P.; Proaño, Carolina B.; Anderson, David J.; Afanasyev, Vsevolod; Wikelski, Martin

2013-01-01

Strong and predictable environmental variability can reward flexible behaviors among animals. We used long-term records of activity data that cover several lunar cycles to investigate whether behavior at-sea of swallow-tailed gulls Creagrus furcatus, a nocturnal pelagic seabird, varied with lunar phase in the Galápagos Islands. A Bayesian hierarchical model showed that nighttime at-sea activity of 37 breeding swallow-tailed gulls was clearly associated with changes in moon phase. Proportion of nighttime spent on water was highest during darker periods of the lunar cycle, coinciding with the cycle of the diel vertical migration (DVM) that brings prey to the sea surface at night. Our data show that at-sea behavior of a tropical seabird can vary with environmental changes, including lunar phase.

At-sea behavior varies with lunar phase in a nocturnal pelagic seabird, the swallow-tailed gull.

PubMed

Cruz, Sebastian M; Hooten, Mevin; Huyvaert, Kathryn P; Proaño, Carolina B; Anderson, David J; Afanasyev, Vsevolod; Wikelski, Martin

2013-01-01

Strong and predictable environmental variability can reward flexible behaviors among animals. We used long-term records of activity data that cover several lunar cycles to investigate whether behavior at-sea of swallow-tailed gulls Creagrus furcatus, a nocturnal pelagic seabird, varied with lunar phase in the Galápagos Islands. A Bayesian hierarchical model showed that nighttime at-sea activity of 37 breeding swallow-tailed gulls was clearly associated with changes in moon phase. Proportion of nighttime spent on water was highest during darker periods of the lunar cycle, coinciding with the cycle of the diel vertical migration (DVM) that brings prey to the sea surface at night. Our data show that at-sea behavior of a tropical seabird can vary with environmental changes, including lunar phase.

IGBT Switching Characteristic Curve Embedded Half-Bridge MMC Modelling and Real Time Simulation Realization

NASA Astrophysics Data System (ADS)

Zhengang, Lu; Hongyang, Yu; Xi, Yang

2017-05-01

The Modular Multilevel Converter (MMC) is one of the most attractive topologies in recent years for medium or high voltage industrial applications, such as high voltage dc transmission (HVDC) and medium voltage varying speed motor drive. The wide adoption of MMCs in industry is mainly due to its flexible expandability, transformer-less configuration, common dc bus, high reliability from redundancy, and so on. But, when the sub module number of MMC is more, the test of MMC controller will cost more time and effort. Hardware in the loop test based on real time simulator will save a lot of time and money caused by the MMC test. And due to the flexible of HIL, it becomes more and more popular in the industry area. The MMC modelling method remains an important issue for the MMC HIL test. Specifically, the VSC model should realistically reflect the nonlinear device switching characteristics, switching and conduction losses, tailing current, and diode reverse recovery behaviour of a realistic converter. In this paper, an IGBT switching characteristic curve embedded half-bridge MMC modelling method is proposed. This method is based on the switching curve referring and sample circuit calculation, and it is sample for implementation. Based on the proposed method, a FPGA real time simulation is carried out with 200ns sample time. The real time simulation results show the proposed method is correct.

Continuous analysis of nitrogen dioxide in gas streams of plants

NASA Technical Reports Server (NTRS)

Durkin, W. T.; Kispert, R. C.

1969-01-01

Analyzer and sampling system continuously monitors nitrogen dioxide concentrations in the feed and tail gas streams of a facility recovering nitric acid. The system, using a direct calorimetric approach, makes use of readily available equipment and is flexible and reliable in operation.

Selective observation of the disordered import signal of a globular protein by in-cell NMR: The example of frataxins

PubMed Central

Popovic, Matija; Sanfelice, Domenico; Pastore, Chiara; Prischi, Filippo; Temussi, Piero Andrea; Pastore, Annalisa

2015-01-01

We have exploited the capability of in-cell NMR to selectively observe flexible regions within folded proteins to carry out a comparative study of two members of the highly conserved frataxin family which are found both in prokaryotes and in eukaryotes. They all contain a globular domain which shares more than 50% identity, which in eukaryotes is preceded by an N-terminal tail containing the mitochondrial import signal. We demonstrate that the NMR spectrum of the bacterial ortholog CyaY cannot be observed in the homologous E. coli system, although it becomes fully observable as soon as the cells are lysed. This behavior has been observed for several other compact globular proteins as seems to be the rule rather than the exception. The NMR spectrum of the yeast ortholog Yfh1 contains instead visible signals from the protein. We demonstrate that they correspond to the flexible N-terminal tail indicating that this is flexible and unfolded. This flexibility of the N-terminus agrees with previous studies of human frataxin, despite the extensive sequence diversity of this region in the two proteins. Interestingly, the residues that we observe in in-cell experiments are not visible in the crystal structure of a Yfh1 mutant designed to destabilize the first helix. More importantly, our results show that, in cell, the protein is predominantly present not as an aggregate but as a monomeric species. PMID:25772583

The use of one- versus two-tailed tests to evaluate prevention programs.

PubMed

Ringwalt, Chris; Paschall, M J; Gorman, Dennis; Derzon, James; Kinlaw, Alan

2011-06-01

Investigators have used both one- and two-tailed tests to determine the significance of findings yielded by program evaluations. While the literature that addresses the appropriate use of each type of significance test should be used is historically inconsistent, almost all authorities now agree that one-tailed tests are rarely (if ever) appropriate. A review of 85 published evaluations of school-based drug prevention curricula specified on the National Registry of Effective Programs and Practices revealed that 20% employed one-tailed tests and, within this subgroup, an additional 4% also employed two-tailed tests. The majority of publications either did not specify the type of statistical test employed or used some other criterion such as effect sizes or confidence intervals. Evaluators reported that they used one-tailed tests either because they stipulated the direction of expected findings in advance, or because prior evaluations of similar programs had yielded no negative results. The authors conclude that one-tailed tests should never be used because they introduce greater potential for Type I errors and create an uneven playing field when outcomes are compared across programs. The authors also conclude that the traditional threshold of significance that places α at .05 is arbitrary and obsolete, and that evaluators should consistently report the exact p values they find.

Torsion and Bending in the Neck and Tail of Sauropod Dinosaurs and the Function of Cervical Ribs: Insights from Functional Morphology and Biomechanics

PubMed Central

Preuschoft, Holger; Klein, Nicole

2013-01-01

The long necks of sauropods have been subject to many studies regarding their posture and flexibility. Length of the neck varies among groups. Here, we investigate neck posture and morphology in several clades from a mechanical viewpoint. Emphasis is put on comparing sauropod necks and tails with structures in living archosaurs and mammals. Differences in the use made of necks and tails lead to clear-cut differences in the mechanical loads occurring in the same models. Ways of sustaining loads are identified by theoretical considerations. If the observed skeletal structures are suited to resist the estimated loading in a particular posture, this concordance is taken as an argument that this posture or movement was of importance during the life of the individual. Apart from the often-discussed bending in side view, we analyze the often overlooked torsion. Because torsional stresses in a homogenous element concentrate near the periphery, a cylindrical cross section gives greatest strength, and the direction of forces is oblique. In a vertebrate neck, during e.g. shaking the head and twisting the neck, oblique muscles, like the mm. scaleni, if activated unilaterally initiate movement, counterbalance the torsional moments and keep the joints between neck vertebrae in equilibrium. If activated bilaterally, these muscles keep the neck balanced in an energy-saving upright posture. The tendons of the mm. scaleni may have ossified as cervical ribs The long cervical ribs in brachiosaurids and mamenchisaurids seem to have limited flexibility, whereas the shorter cervical ribs in Diplodocidae allowed free movement. The tails of sauropods do not show pronounced adaptation to torsion, and seem to have been carried more or less in a horizontal, extended posture. In this respect, sauropod tails resemble the necks of herbivorous cursorial mammals. These analyses provide an improved understanding of neck use that will be extended to other sauropods in subsequent studies. PMID:24205268

Acute and repeated activation of male sexual behavior by tail pinch: opioid and dopaminergic mechanisms.

PubMed

Leyton, M; Stewart, J

1996-07-01

We studied the effect of tail pinch on male sexual behavior and its neurochemical basis. Male rats were gonadectomized and maintained on low doses of testosterone propionate (20.0 micrograms/day). Tail pinch significantly increased the percentage of males that mounted, intromitted, and ejaculated within a 30-min test, and these increases were attenuated by both pimozide (1.0 mg/kg, i.p.) and by naloxone (0.5, 1.0, and 2.0 mg/kg, s.c.). Moreover, tail pinch in the presence of an estrous female led to significantly increased female-directed behavior 48 h later during a test without tail pinch. Repeated tail pinch tests led to progressively more sexual activity, and the development of this behavioral sensitization was prevented by naloxone. These findings suggest that tail pinch increases the salience of the incentive characteristics of the female. Furthermore, during subsequent tests, with or without tail pinch, the increased salience of the female remains, as measured by the continued increases in sexual activity. These acute and sensitized behavioral increases might result from tail pinch-induced activation of the midbrain dopamine system via an opioid mechanism; either preventing tail pinch-induced dopamine activation (by an opioid antagonist) or blocking the effects of dopamine activation (by a dopamine antagonist) attenuated the long-term facilitation of sexual behavior seen after pairing the female with tail pinch.

An Overview of Modifications Applied to a Turbulence Response Analysis Method for Flexible Aircraft Configurations

NASA Technical Reports Server (NTRS)

Funk, Christie J.

2013-01-01

A software program and associated methodology to study gust loading on aircraft exists for a classification of geometrically simplified flexible configurations. This program consists of a simple aircraft response model with two rigid and three flexible symmetric degrees of freedom and allows for the calculation of various airplane responses due to a discrete one-minus-cosine gust as well as continuous turbulence. Simplifications, assumptions, and opportunities for potential improvements pertaining to the existing software program are first identified, then a revised version of the original software tool is developed with improved methodology to include more complex geometries, additional excitation cases, and output data so as to provide a more useful and accurate tool for gust load analysis. Revisions are made in the categories of aircraft geometry, computation of aerodynamic forces and moments, and implementation of horizontal tail mode shapes. In order to improve the original software program to enhance usefulness, a wing control surface and horizontal tail control surface is added, an extended application of the discrete one-minus-cosine gust input is employed, a supplemental continuous turbulence spectrum is implemented, and a capability to animate the total vehicle deformation response to gust inputs in included. These revisions and enhancements are implemented and an analysis of the results is used to validate the modifications.

Schistosoma mansoni cercariae swim efficiently by exploiting an elastohydrodynamic coupling

NASA Astrophysics Data System (ADS)

Krishnamurthy, Deepak; Katsikis, Georgios; Bhargava, Arjun; Prakash, Manu

2017-03-01

The motility of many parasites is critical for infecting their host, as exemplified in the transmission cycle of the parasite Schistosoma mansoni. In its human infectious stage, submillimetre-scale forms of the parasite known as cercariae swim in freshwater and infect humans by penetrating the skin. This infection causes schistosomiasis, a disease comparable to malaria in global socio-economic impact. Given that cercariae do not feed and hence have a lifetime of around 12 hours, efficient motility is crucial for schistosomiasis transmission. Despite this, a first-principles understanding of how cercariae swim is lacking. Combining biological experiments, a novel theoretical model and its robotic realization, we show that cercariae use their forked tail to swim against gravity using a novel swimming gait, described here as a `T-swimmer gait'. During this gait, cercariae beat their tail periodically while maintaining an increased flexibility near their posterior and anterior ends. This flexibility allows an interaction between fluid drag and bending resistance--an elastohydrodynamic coupling, to naturally break time-reversal symmetry and enable locomotion at small length scales. Finally, we find that cercariae maintain this flexibility at an optimal regime for efficient swimming. We anticipate that our work sets the ground for linking the swimming of cercariae to disease transmission, and could potentially enable explorations of novel strategies for schistosomiasis control and prevention.

At–Sea Behavior Varies with Lunar Phase in a Nocturnal Pelagic Seabird, the Swallow-Tailed Gull

PubMed Central

Cruz, Sebastian M.; Hooten, Mevin; Huyvaert, Kathryn P.; Proaño, Carolina B.; Anderson, David J.; Afanasyev, Vsevolod; Wikelski, Martin

2013-01-01

Strong and predictable environmental variability can reward flexible behaviors among animals. We used long-term records of activity data that cover several lunar cycles to investigate whether behavior at-sea of swallow-tailed gulls Creagrus furcatus, a nocturnal pelagic seabird, varied with lunar phase in the Galápagos Islands. A Bayesian hierarchical model showed that nighttime at-sea activity of 37 breeding swallow-tailed gulls was clearly associated with changes in moon phase. Proportion of nighttime spent on water was highest during darker periods of the lunar cycle, coinciding with the cycle of the diel vertical migration (DVM) that brings prey to the sea surface at night. Our data show that at-sea behavior of a tropical seabird can vary with environmental changes, including lunar phase. PMID:23468889

Wing-Fuselage Interference, Tail Buffeting, and Air Flow About the Tail of a Low-Wing Monoplane

NASA Technical Reports Server (NTRS)

White, James A; Hood, Manley J

1935-01-01

This report presents the results of wind tunnel tests on a Mcdonnell Douglas airplane to determine the wing-fuselage interference of a low-wing monoplane. The tests included a study of tail buffeting and the air flow in the region of the tail. The airplane was tested with and without the propeller slipstream, both in the original condition and with several devices designed to reduce or eliminate tail buffeting. The devices used were wing-fuselage fillets, a NACA cowling, reflexed trailing edge of the wing, and stub auxiliary airfoils.

Tadpole-like artificial micromotor.

PubMed

Liu, Limei; Liu, Mei; Su, Yajun; Dong, Yonggang; Zhou, Wei; Zhang, Lina; Zhang, Hui; Dong, Bin; Chi, Lifeng

2015-02-14

We describe a polymer-based artificial tadpole-like micromotor, which is fabricated through the electrospinning technique. By incorporating functional materials onto its surface or within its body, the resulting tadpole-like micromotor can not only move autonomously in an aqueous solution with a flexible tail, but also exhibit thermo- and magnetic responsive properties.

Development in helicopter tail boom strake applications in the US

NASA Technical Reports Server (NTRS)

Wilson, John C.; Kelley, Henry L.; Donahue, Cynthia C.; Yenni, Kenneth R.

1988-01-01

The use of a strake or spoiler on a helicopter tail boom to beneficially change helicopter tail boom air loads was suggested in the United States in 1975. The anticipated benefits were a change of tail boom loads to reduce required tail rotor thrust and power and improve directional control. High tail boom air loads experienced by the YAH-64 and described in 1978 led to a wind tunnel investigation of the usefullness of strakes in altering such loads on the AH-64, UH-60, and UH-1 helicopters. The wind tunnel tests of 2-D cross sections of the tail boom of each demonstrated that a strake or strakes would be effective. Several limited test programs with the U.S. Army's OH-58A, AH-64, and UH-60A were conducted which showed the effects of strakes were modest for those helicopters. The most recent flight test program, with a Bell 204B, disclosed that for the 204B the tail boom strake or strakes would provide more than a modest improvement in directional control and reduction in tail rotor power.

Hydrodynamic Characteristics of a Low-drag, Planing-tail Flying-boat Hull

NASA Technical Reports Server (NTRS)

Suydam, Henry B

1948-01-01

The hydrodynamic characteristics of a flying-boat incorporating a low-drag, planing-tail hull were determined from model tests made in Langley tank number 2 and compared with tests of the same flying boat incorporating a conventional-type hull. The planing-tail model, with which stable take-offs were possible for a large range of elevator positions at all center-of-gravity locations tested, had more take-off stability than the conventional model. No upper-limit porpoising was encountered by the planing-tail model. The maximum changes in rise during landings were lower for the planing-tail model than for the conventional model at most contact trims, an indication of improved landing stability for the planing-tail model. The hydrodynamic resistance of the planing-tail hull was lower than the conventional hull at all speeds, and the load-resistance ratio was higher for the planing-tail hull, being especially high at the hump. The static trim of the planing-tail hull was much higher than the conventional hull, but the variation of trim with speed during take-off was smaller.

Effect of Dynamic Rolling Oscillations on Twin Tail Buffet Response

NASA Technical Reports Server (NTRS)

Sheta, Essam F.; Kandil, Osama A.

1999-01-01

The effect of dynamic rolling oscillations of delta-wing/twin-tail configuration on twin-tail buffet response is investigated. The computational model consists of a sharp-edged delta wing of aspect ratio one and swept-back flexible twin tail with taper ratio of 0.23. The configuration model is statically pitched at 30 deg. angle of attack and then forced to oscillate in roll around the symmetry axis at a constant amplitude of 4 deg. and reduced frequency of pi and 2(pi). The freestream Mach number and Reynolds number are 0.3 and 1.25 million, respectively. This multidisciplinary problem is solved using three sets of equations on a dynamic multi-block grid structure. The first set is the unsteady, full Navier-Stokes equations, the second set is the aeroelastic equations for coupled bending and torsion vibrations of the tails, and the third set is the grid-displacement equations. The configuration is investigated for inboard position of the twin tails which corresponds to a separation distance between the twin tails of 33% wing span. The computed results are compared with the results of stationary configuration, which previously have been validated using experimental data. The results conclusively showed that the rolling oscillations of the configuration have led to higher loads, higher deflections, and higher excitation peaks than those of the stationary configuration. Moreover, increasing the reduced frequency has led to higher loads and excitation peaks and lower bending and torsion deflections and acceleration.

Effects of aerodynamic interaction between main and tail rotors on helicopter hover performance and noise

NASA Technical Reports Server (NTRS)

Menger, R. P.; Wood, T. L.; Brieger, J. T.

1983-01-01

A model test was conducted to determine the effects of aerodynamic interaction between main rotor, tail rotor, and vertical fin on helicopter performance and noise in hover out of ground effect. The experimental data were obtained from hover tests performed with a .151 scale Model 222 main rotor, tail rotor and vertical fin. Of primary interest was the effect of location of the tail rotor with respect to the main rotor. Penalties on main rotor power due to interaction with the tail rotor ranged up to 3% depending upon tail rotor location and orientation. Penalties on tail rotor power due to fin blockage alone ranged up to 10% for pusher tail rotors and up to 50% for tractor tail rotors. The main rotor wake had only a second order effect on these tail rotor/fin interactions. Design charts are presented showing the penalties on main rotor power as a function of the relative location of the tail rotor.

Structured Post-IQ Domain Governs Selectivity of Myosin X for Fascin-Actin Bundles*

PubMed Central

Nagy, Stanislav; Rock, Ronald S.

2010-01-01

Without guidance cues, cytoskeletal motors would traffic components to the wrong destination with disastrous consequences for the cell. Recently, we identified a motor protein, myosin X, that identifies bundled actin filaments for transport. These bundles direct myosin X to a unique destination, the tips of cellular filopodia. Because the structural and kinetic features that drive bundle selection are unknown, we employed a domain-swapping approach with the nonselective myosin V to identify the selectivity module of myosin X. We found a surprising role of the myosin X tail region (post-IQ) in supporting long runs on bundles. Moreover, the myosin X head is adapted for initiating processive runs on bundles. We found that the tail is structured and biases the orientation of the two myosin X heads because a targeted insertion that introduces flexibility in the tail abolishes selectivity. Together, these results suggest how myosin motors may manage to read cellular addresses. PMID:20538587

Predicting arsenic concentrations in porewaters of buried uranium mill tailings

DOE Office of Scientific and Technical Information (OSTI.GOV)

Langmuir, D.; Mahoney, J.; MacDonald, A.

The proposed JEB Tailings Management Facility (TMF) to be emplaced below the groundwater table in northern Saskatchewan, Canada, will contain uranium mill tailings from McClean Lake, Midwest and Cigar Lake ore bodies, which are high in arsenic (up to 10%) and nickel (up to 5%). A serious concern is the possibility that high arsenic and nickel concentrations may be released from the buried tailings, contaminating adjacent groundwaters and a nearby lake. Laboratory tests and geochemical modeling were performed to examine ways to reduce the arsenic and nickel concentrations in TMF porewaters so as to minimize such contamination from tailings buriedmore » for 50 years and longer. The tests were designed to mimic conditions in the mill neutralization circuit (3 hr tests at 25 C), and in the TMF after burial (5--49 day aging tests). The aging tests were run at 50, 25 and 4 C (the temperature in the TMF). In order to optimize the removal of arsenic by adsorption and precipitation, ferric sulfate was added to tailings raffinates having Fe/As ratios of less than 3--5. The acid raffinates were then neutralized by addition of slaked lime to nominal pH values of 7, 8, or 9. Analysis and modeling of the test results showed that with slaked lime addition to acid tailings raffinates, relatively amorphous scorodite (ferric arsenate) precipitates near pH 1, and is the dominant form of arsenate in slake limed tailings solids except those high in Ni and As and low in Fe, in which cabrerite-annabergite (Ni, Mg, Fe(II) arsenate) may also precipitate near pH 5--6. In addition to the arsenate precipitates, smaller amounts of arsenate are also adsorbed onto tailings solids. The aging tests showed that after burial of the tailings, arsenic concentrations may increase with time from the breakdown of the arsenate phases (chiefly scorodite). However, the tests indicate that the rate of change decreases and approaches zero after 72 hrs at 25 C, and may equal zero at all times in the TMF at 4 C. Consistent with a kinetic model that describes the rate of breakdown of scorodite to form hydrous ferric oxide, the rate of release of dissolved arsenate to tailings porewaters from slake limed tailings: (1) is proportional to pH above pH 6--7; (2) decreases exponentially as the total molar Fe/As ratio of tailings raffinates is increased from 1/1 to greater than 5/1; and (3) is proportional to temperature with an average Arrhenius activation energy of 13.4 {+-} 4.2 kcal/mol. Study results suggest that if ferric sulfate and slaked lime are added in the tailings neutralization circuit to give a raffinate Fe/As molar ratio of at least 3--5 and a nominal (initial) pH of 8 (final pH of 7--8), arsenic and nickel concentrations of 2 mg/L or less, are probable in porewaters of individual tailings in the TMF for 50 to 10,000 yrs after tailings disposal. However, the tailings will be mixed in the TMF, which will contain about 35% tailings with Fe/As = 3.0, and 65% tailings with Fe/As = 5.0--7.7. Thus, it seems likely that average arsenic pore water concentrations in the TMF may not exceed 1 mg/L.« less

Trimodal Control of Ion-Transport Activity on Cyclo-oligo-(1→6)-β-D-glucosamine-Based Artificial Ion-Transport Systems.

PubMed

Roy, Arundhati; Saha, Tanmoy; Gening, Marina L; Titov, Denis V; Gerbst, Alexey G; Tsvetkov, Yury E; Nifantiev, Nikolay E; Talukdar, Pinaki

2015-11-23

Cyclo-oligo-(1→6)-β-D-glucosamines functionalized with hydrophobic tails are reported as a new class of transmembrane ion-transport system. These macrocycles with hydrophilic cavities were introduced as an alternative to cyclodextrins, which are supramolecular systems with hydrophobic cavities. The transport activities of these glycoconjugates were manipulated by altering the oligomericity of the macrocycles, as well as the length and number of attached tails. Hydrophobic tails of 3 different sizes were synthesized and coupled with each glucosamine scaffold through the amide linkage to obtain 18 derivatives. The ion-transport activity increased from di- to tetrameric glucosamine macrocycles, but decreased further when flexible pentameric glucosamine was introduced. The ion-transport activity also increased with increasing length of attached linkers. For a fixed length of linkers, the transport activity decreased when the number of such tails was reduced. All glycoconjugates displayed a uniform anion-selectivity sequence: Cl(-) >Br(-) >I(-) . From theoretical studies, hydrogen bonding between the macrocycle backbone and the anion bridged through water molecules was observed. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

A multiscale approach to simulating the conformational properties of unbound multi-C₂H₂ zinc finger proteins.

PubMed

Liu, Lei; Wade, Rebecca C; Heermann, Dieter W

2015-09-01

The conformational properties of unbound multi-Cys2 His2 (mC2H2) zinc finger proteins, in which zinc finger domains are connected by flexible linkers, are studied by a multiscale approach. Three methods on different length scales are utilized. First, atomic detail molecular dynamics simulations of one zinc finger and its adjacent flexible linker confirmed that the zinc finger is more rigid than the flexible linker. Second, the end-to-end distance distributions of mC2H2 zinc finger proteins are computed using an efficient atomistic pivoting algorithm, which only takes excluded volume interactions into consideration. The end-to-end distance distribution gradually changes its profile, from left-tailed to right-tailed, as the number of zinc fingers increases. This is explained by using a worm-like chain model. For proteins of a few zinc fingers, an effective bending constraint favors an extended conformation. Only for proteins containing more than nine zinc fingers, is a somewhat compacted conformation preferred. Third, a mesoscale model is modified to study both the local and the global conformational properties of multi-C2H2 zinc finger proteins. Simulations of the CCCTC-binding factor (CTCF), an important mC2H2 zinc finger protein for genome spatial organization, are presented. © 2015 Wiley Periodicals, Inc.

Flexible ultrasonic pipe inspection apparatus

DOEpatents

Jenkins, C.F.; Howard, B.D.

1998-06-23

A flexible, modular ultrasonic pipe inspection apparatus, comprises a flexible, hollow shaft that carries a plurality of modules, including at least one rotatable ultrasonic transducer, a motor/gear unit, and a position/signal encoder. The modules are connected by flexible knuckle joints that allow each module of the apparatus to change its relative orientation with respect to a neighboring module, while the shaft protects electrical wiring from kinking or buckling while the apparatus moves around a tight corner. The apparatus is moved through a pipe by any suitable means, including a tether or drawstring attached to the nose or tail, differential hydraulic pressure, or a pipe pig. The rotational speed of the ultrasonic transducer and the forward velocity of the apparatus are coordinated so that the beam sweeps out the entire interior surface of the pipe, enabling the operator to accurately assess the condition of the pipe wall and determine whether or not leak-prone corrosion damage is present. 7 figs.

Flexible ultrasonic pipe inspection apparatus

DOEpatents

Jenkins, Charles F.; Howard, Boyd D.

1998-01-01

A flexible, modular ultrasonic pipe inspection apparatus, comprising a flexible, hollow shaft that carries a plurality of modules, including at least one rotatable ultrasonic transducer, a motor/gear unit, and a position/signal encoder. The modules are connected by flexible knuckle joints that allow each module of the apparatus to change its relative orientation with respect to a neighboring module, while the shaft protects electrical wiring from kinking or buckling while the apparatus moves around a tight corner. The apparatus is moved through a pipe by any suitable means, including a tether or drawstring attached to the nose or tail, differential hydraulic pressure, or a pipe pig. The rotational speed of the ultrasonic transducer and the forward velocity of the apparatus are coordinated so that the beam sweeps out the entire interior surface of the pipe, enabling the operator to accurately assess the condition of the pipe wall and determine whether or not leak-prone corrosion damage is present.

Limit cycles of a flexible shaft with hydrodynamic journal bearings in unstable regimes

NASA Technical Reports Server (NTRS)

Brown, R. D.; Black, H. F.

1980-01-01

A symmetric 3 mass rotor supported on hydrodynamic bearings is described. An approximate method of representing finite bearings is used to calculate bearing forces. As the method sums forces from a number of independent circular lobes lemon 3 and 4 lobe bearings are taken into account. The calculations are based on an axial groove bearing. Linear analysis precedes nonlinear simulation of some unstable conditions. The demonstration of small limit cycles suggests that necessarily flexible rotors e.g., helicopter tail rotors, may be practical without either tilt pad bearings or external dampers.

Supplementary Free-Spinning-Tunnel Tests of a 1/16-Scale Model of the McDonnell XB-85 Airplane Equipped with a Conventional-Tail Arrangement

NASA Technical Reports Server (NTRS)

Klinar, Walter J.

1947-01-01

Spin tests have been conducted in the Langley free-spinning tunnel on a 1/16-scale model of the McDonnell XP-85 airplane with the normal X-tail replaced with a short-coupled conventional tail arrangement. The effect of the conventional tail arrangement and the effects of various modifications upon the spin and recovery characteristics of the model were determined. The results of the tests indicated that installation of the conventional tail arrangement wil not provide satisfactory recoveries from spins of the airplane. Satisfactory recoveries will be obtainable, however, either by installing in addition a very large ventral fin (17.94 sq ft, full-scale) below the tail or by decreasing the width of the fuselage and making it flat sided rearward of the wing trailing edge.

Recycling of asbestos tailings used as reinforcing fillers in polypropylene based composites.

PubMed

Zhai, Wensi; Wang, Yao; Deng, Yuan; Gao, Hongli; Lin, Zhen; Li, Mao

2014-04-15

In this work, asbestos tailings were recycled and used as reinforcing fillers to enhance the mechanical properties of polypropylene (PP). A silane coupling agent was used to chemically modify the asbestos tailings to increase the compatibility between asbestos tailings and polypropylene matrix. Both raw and chemically treated asbestos tailings with different loading levels (from 3 to 30 wt%) were utilized to fabricate composites. Mechanical properties of these composites have been investigated by dynamic mechanical analysis, tensile test and notched impact test. Results showed that hybridization of asbestos tailings in the composites enhanced the mechanical properties of neat PP evidently, and treated asbestos tailings/PP composites yielded even better mechanical properties compared with those of raw asbestos tailings/PP composites. This recycling method of asbestos tailings not only reduces disposal costs and avoids secondary pollution but also produces a new PP-based composite material with enhanced mechanical properties. Copyright © 2014 Elsevier B.V. All rights reserved.

Experimental study of main rotor tip geometry and tail rotor interactions in hover. Volume 1. Text and figures

NASA Technical Reports Server (NTRS)

Balch, D. T.; Lombardi, J.

1985-01-01

A model scale hover test was conducted in the Sikorsky Aircraft Model rotor hover Facility to identify and quantify the impact of the tail rotor on the demonstrated advantages of advanced geometry tip configurations. The test was conducted using the Basic Model Test Rig and two scaled main rotor systems, one representing a 1/5.727 scale UH-60A BLACK HAWK and the others a 1/4.71 scale S-76. Eight alternate rotor tip configurations were tested, 3 on the BLACK HAWK rotor and 6 on the S-76 rotor. Four of these tips were then selected for testing in close proximity to an operating tail rotor (operating in both tractor and pusher modes) to determine if the performance advantages that could be obtained from the use of advanced geometry tips in a main rotor only environment would still exist in the more complex flow field involving a tail rotor. The test showed that overall the tail rotor effects on the advanced tip configurations tested are not substantially different from the effects on conventional tips.

Ice Accretion Calculations for a Commercial Transport Using the LEWICE3D, ICEGRID3D and CMARC Programs

NASA Technical Reports Server (NTRS)

Bidwell, Colin S.; Pinella, David; Garrison, Peter

1999-01-01

Collection efficiency and ice accretion calculations were made for a commercial transport using the NASA Lewis LEWICE3D ice accretion code, the ICEGRID3D grid code and the CMARC panel code. All of the calculations were made on a Windows 95 based personal computer. The ice accretion calculations were made for the nose, wing, horizontal tail and vertical tail surfaces. Ice shapes typifying those of a 30 minute hold were generated. Collection efficiencies were also generated for the entire aircraft using the newly developed unstructured collection efficiency method. The calculations highlight the flexibility and cost effectiveness of the LEWICE3D, ICEGRID3D, CMARC combination.

When Siberia came to the Netherlands: The response of continental black-tailed godwits to a rare spring weather event

USGS Publications Warehouse

Senner, Nathan R.; Verhoeven, Mo A.; Abad-Gómez, José M.; Gutiérrez, Jorge S.; Hooijmeijer, Jos C. E. W.; Kentie, Rosemarie; Masero, José A.; Tibbitts, T. Lee; Piersma, Theunis

2015-01-01

This suggests that populations with continued access to food, behavioural flexibility and time to dissipate the costs of the event can likely withstand the consequences of an extreme weather event. For populations constrained in one of these respects, though, extreme events may entail extreme ecological consequences.

Ground/Flight Correlation of Aerodynamic Loads with Structural Response

NASA Technical Reports Server (NTRS)

Mangalam, Arun S.; Davis, Mark C.

2009-01-01

United States Air Force Research Laboratory (AFRL) ground tests at the NASA Transonic Dynamics Tunnel (TDT) and NASA flight tests provide a basis and methodology for in-flight characterization of the aeroelastic performance through the monitoring of the fluid-structure interaction using surface flow sensors. NASA NF-15B flight tests provided a unique opportunity to test the correlation of aerodynamic loads with sectional flow attachment/detachment points, also known as flow bifurcation points (FBPs), as observed in previous wind tunnel tests. The NF-15B tail was instrumented with hot-film sensors and strain gages for measuring root-bending strains. These data were gathered via selected sideslip maneuvers performed at level flight and subsonic speeds. The aerodynamic loads generated by the sideslip maneuver resulted in root-bending strains and hot-film sensor signals near the stagnation region that were highly correlated. For the TDT tests, a flexible wing section developed under the AFRL SensorCraft program was instrumented with strain gages, accelerometers, and hot-film sensors at multiple span stations. The TDT tests provided data showing a gradual phase change between the FBP and the structural mode occurred during a resonant condition as the wings structural modes were excited by the tunnel-generated gusts.

Wind-Tunnel Investigation of Effects of Unsymmetrical Horizontal-Tail Arrangements on Power-on Static Longitudinal Stability of a Single-Engine Airplane Model

NASA Technical Reports Server (NTRS)

Purser, Paul E.; Spear, Margaret F.

1947-01-01

A wind-tunnel investigation has been made to determine the effects of unsymmetrical horizontal-tail arrangements on the power-on static longitudinal stability of a single-engine single-rotation airplane model. Although the tests and analyses showed that extreme asymmetry in the horizontal tail indicated a reduction in power effects on longitudinal stability for single-engine single-rotation airplanes, the particular "practical" arrangement tested did not show marked improvement. Differences in average downwash between the normal tail arrangement and various other tail arrangements estimated from computed values of propeller-slipstream rotation agreed with values estimated from pitching-moment test data for the flaps-up condition (low thrust and torque) and disagreed for the flaps-down condition (high thrust and torque). This disagreement indicated the necessity for continued research to determine the characteristics of the slip-stream behind various propeller-fuselage-wing combinations. Out-of-trim lateral forces and moments of the unsymmetrical tail arrangements that were best from consideration of longitudinal stability were no greater than those of the normal tail arrangement.

A study of helicopter stability and control including blade dynamics

NASA Technical Reports Server (NTRS)

Zhao, Xin; Curtiss, H. C., Jr.

1988-01-01

A linearized model of rotorcraft dynamics has been developed through the use of symbolic automatic equation generating techniques. The dynamic model has been formulated in a unique way such that it can be used to analyze a variety of rotor/body coupling problems including a rotor mounted on a flexible shaft with a number of modes as well as free-flight stability and control characteristics. Direct comparison of the time response to longitudinal, lateral and directional control inputs at various trim conditions shows that the linear model yields good to very good correlation with flight test. In particular it is shown that a dynamic inflow model is essential to obtain good time response correlation, especially for the hover trim condition. It also is shown that the main rotor wake interaction with the tail rotor and fixed tail surfaces is a significant contributor to the response at translational flight trim conditions. A relatively simple model for the downwash and sidewash at the tail surfaces based on flat vortex wake theory is shown to produce good agreement. Then, the influence of rotor flap and lag dynamics on automatic control systems feedback gain limitations is investigated with the model. It is shown that the blade dynamics, especially lagging dynamics, can severly limit the useable values of the feedback gain for simple feedback control and that multivariable optimal control theory is a powerful tool to design high gain augmentation control system. The frequency-shaped optimal control design can offer much better flight dynamic characteristics and a stable margin for the feedback system without need to model the lagging dynamics.

The Effects of Horizontal-Tail Location and Wing Modifications on the High-Speed Stability and Control Characteristics of a 01.17-Scale Model of the McDonnell XF2H-1 Airplane (TED No, NACA DE336)

NASA Technical Reports Server (NTRS)

Emerson, Horace F.; Axelson, John A.

1949-01-01

An additional series of high-speed wind-tunnel tests of a modified 0.17-scale model of the McDonnell XF2H-1 airplane was conducted to evaluate the effects of a reduction in the thickness-to-chord ratios of the tail planes, the displacement of the horizontal tail relative to the vertical tail, and the extension of the trailing edge of the wing. Two tail-intersection fairings designed to improve the flow at the tail were also tested. The pitching-moment characteristics of the model were improved slightly by the use of the thinner tail sections. Rearward or rearward and downward displacements of the horizontal tail increased the critical Mach number at the tail intersection from 0.725 to a maximum of 0.80, but caused an excessive change in pitching-moment coefficient at the higher Mach numbers. Extending the trailing edge of the wing did not improve the static longitudinal-stability characteristics, but increased the pitching-down tendency between 0.725 and 0.825 Mach numbers prior to the pitching-up tendency. The extended wing did, however, increase the Mach numbers at which these tendencies occurred. The increase in the Mach numbers of divergence and the tuft studies indicate a probable increase in the buffet limit of the prototype airplane. No perceptible improvement of flow at the tail intersection was observed with the two fairings tested on the forward tail configuration.

L-moments and TL-moments of the generalized lambda distribution

USGS Publications Warehouse

Asquith, W.H.

2007-01-01

The 4-parameter generalized lambda distribution (GLD) is a flexible distribution capable of mimicking the shapes of many distributions and data samples including those with heavy tails. The method of L-moments and the recently developed method of trimmed L-moments (TL-moments) are attractive techniques for parameter estimation for heavy-tailed distributions for which the L- and TL-moments have been defined. Analytical solutions for the first five L- and TL-moments in terms of GLD parameters are derived. Unfortunately, numerical methods are needed to compute the parameters from the L- or TL-moments. Algorithms are suggested for parameter estimation. Application of the GLD using both L- and TL-moment parameter estimates from example data is demonstrated, and comparison of the L-moment fit of the 4-parameter kappa distribution is made. A small simulation study of the 98th percentile (far-right tail) is conducted for a heavy-tail GLD with high-outlier contamination. The simulations show, with respect to estimation of the 98th-percent quantile, that TL-moments are less biased (more robost) in the presence of high-outlier contamination. However, the robustness comes at the expense of considerably more sampling variability. ?? 2006 Elsevier B.V. All rights reserved.

Structural model of dodecameric heat-shock protein Hsp21: Flexible N-terminal arms interact with client proteins while C-terminal tails maintain the dodecamer and chaperone activity.

PubMed

Rutsdottir, Gudrun; Härmark, Johan; Weide, Yoran; Hebert, Hans; Rasmussen, Morten I; Wernersson, Sven; Respondek, Michal; Akke, Mikael; Højrup, Peter; Koeck, Philip J B; Söderberg, Christopher A G; Emanuelsson, Cecilia

2017-05-12

Small heat-shock proteins (sHsps) prevent aggregation of thermosensitive client proteins in a first line of defense against cellular stress. The mechanisms by which they perform this function have been hard to define due to limited structural information; currently, there is only one high-resolution structure of a plant sHsp published, that of the cytosolic Hsp16.9. We took interest in Hsp21, a chloroplast-localized sHsp crucial for plant stress resistance, which has even longer N-terminal arms than Hsp16.9, with a functionally important and conserved methionine-rich motif. To provide a framework for investigating structure-function relationships of Hsp21 and understanding these sequence variations, we developed a structural model of Hsp21 based on homology modeling, cryo-EM, cross-linking mass spectrometry, NMR, and small-angle X-ray scattering. Our data suggest a dodecameric arrangement of two trimer-of-dimer discs stabilized by the C-terminal tails, possibly through tail-to-tail interactions between the discs, mediated through extended I X V X I motifs. Our model further suggests that six N-terminal arms are located on the outside of the dodecamer, accessible for interaction with client proteins, and distinct from previous undefined or inwardly facing arms. To test the importance of the I X V X I motif, we created the point mutant V181A, which, as expected, disrupts the Hsp21 dodecamer and decreases chaperone activity. Finally, our data emphasize that sHsp chaperone efficiency depends on oligomerization and that client interactions can occur both with and without oligomer dissociation. These results provide a generalizable workflow to explore sHsps, expand our understanding of sHsp structural motifs, and provide a testable Hsp21 structure model to inform future investigations. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Grasping convergent evolution in syngnathids: a unique tale of tails.

PubMed

Neutens, C; Adriaens, D; Christiaens, J; De Kegel, B; Dierick, M; Boistel, R; Van Hoorebeke, L

2014-06-01

Seahorses and pipehorses both possess a prehensile tail, a unique characteristic among teleost fishes, allowing them to grasp and hold onto substrates such as sea grasses. Although studies have focused on tail grasping, the pattern of evolutionary transformations that made this possible is poorly understood. Recent phylogenetic studies show that the prehensile tail evolved independently in different syngnathid lineages, including seahorses, Haliichthys taeniophorus and several types of so-called pipehorses. This study explores the pattern that characterizes this convergent evolution towards a prehensile tail, by comparing the caudal musculoskeletal organization, as well as passive bending capacities in pipefish (representing the ancestral state), pipehorse, seahorse and H. taeniophorus. To study the complex musculoskeletal morphology, histological sectioning, μCT-scanning and phase contrast synchrotron scanning were combined with virtual 3D-reconstructions. Results suggest that the independent evolution towards tail grasping in syngnathids reflects at least two quite different strategies in which the ancestral condition of a heavy plated and rigid system became modified into a highly flexible one. Intermediate skeletal morphologies (between the ancestral condition and seahorses) could be found in the pygmy pipehorses and H. taeniophorus, which are phylogenetically closely affiliated with seahorses. This study suggests that the characteristic parallel myoseptal organization as already described in seahorse (compared with a conical organization in pipefish and pipehorse) may not be a necessity for grasping, but represents an apomorphy for seahorses, as this pattern is not found in other syngnathid species possessing a prehensile tail. One could suggest that the functionality of grasping evolved before the specialized, parallel myoseptal organization seen in seahorses. However, as the grasping system in pipehorses is a totally different one, this cannot be concluded from this study. © 2014 Anatomical Society.

Grasping convergent evolution in syngnathids: a unique tale of tails

PubMed Central

Neutens, C; Adriaens, D; Christiaens, J; De Kegel, B; Dierick, M; Boistel, R; Van Hoorebeke, L

2014-01-01

Seahorses and pipehorses both possess a prehensile tail, a unique characteristic among teleost fishes, allowing them to grasp and hold onto substrates such as sea grasses. Although studies have focused on tail grasping, the pattern of evolutionary transformations that made this possible is poorly understood. Recent phylogenetic studies show that the prehensile tail evolved independently in different syngnathid lineages, including seahorses, Haliichthys taeniophorus and several types of so-called pipehorses. This study explores the pattern that characterizes this convergent evolution towards a prehensile tail, by comparing the caudal musculoskeletal organization, as well as passive bending capacities in pipefish (representing the ancestral state), pipehorse, seahorse and H. taeniophorus. To study the complex musculoskeletal morphology, histological sectioning, μCT-scanning and phase contrast synchrotron scanning were combined with virtual 3D-reconstructions. Results suggest that the independent evolution towards tail grasping in syngnathids reflects at least two quite different strategies in which the ancestral condition of a heavy plated and rigid system became modified into a highly flexible one. Intermediate skeletal morphologies (between the ancestral condition and seahorses) could be found in the pygmy pipehorses and H. taeniophorus, which are phylogenetically closely affiliated with seahorses. This study suggests that the characteristic parallel myoseptal organization as already described in seahorse (compared with a conical organization in pipefish and pipehorse) may not be a necessity for grasping, but represents an apomorphy for seahorses, as this pattern is not found in other syngnathid species possessing a prehensile tail. One could suggest that the functionality of grasping evolved before the specialized, parallel myoseptal organization seen in seahorses. However, as the grasping system in pipehorses is a totally different one, this cannot be concluded from this study. PMID:24697519

Wind-tunnel investigation at supersonic speeds of a remote-controlled canard missile with a free-rolling-tail brake torque system

NASA Technical Reports Server (NTRS)

Blair, A. B., Jr.

1985-01-01

Wind tunnel tests were conducted at Mach numbers 1.70, 2.16, and 2.86 to determine the static aerodynamic characteristics of a cruciform canard-controlled missile with fixed or free rolling tailfin afterbodies. Mechanical coupling effects of the free-rolling-tail afterbody were investigated by using an electronic electromagnetic brake system providing arbitrary tail-fin brake torques with continuous measurements of tail-to-mainframe torque and tail roll rate. Remote-controlled canards were deflected to provide pitch, yaw, and roll control. Results indicate that the induced rolling moment coefficients due to canard yaw control are reduced and linearized for the free-rolling-tail (free-tail) configuration. The canards of the latter provide conventional roll control for the entire angle-of-attack test range. For the free-tail configuration, the induced rolling moment coefficient due to canard yaw control increased and the canard roll control decreased with increases in brake torque, which simulated bearing friction torque. It appears that a compromise in regard to bearing friction, for example, low-cost bearings with some friction, may allow satisfactory free-tail aerodynamic characteristics that include reductions in adverse rolling-moment coefficients and lower tail roll rates.

Structural analysis of the complex between influenza B nucleoprotein and human importin-α.

PubMed

Labaronne, Alice; Milles, Sigrid; Donchet, Amélie; Jensen, Malene Ringkjøbing; Blackledge, Martin; Bourhis, Jean-Marie; Ruigrok, Rob W H; Crépin, Thibaut

2017-12-07

Influenza viruses are negative strand RNA viruses that replicate in the nucleus of the cell. The viral nucleoprotein (NP) is the major component of the viral ribonucleoprotein. In this paper we show that the NP of influenza B has a long N-terminal tail of 70 residues with intrinsic flexibility. This tail contains the Nuclear Location Signal (NLS). The nuclear trafficking of the viral components mobilizes cellular import factors at different stages, making these host-pathogen interactions promising targets for new therapeutics. NP is imported into the nucleus by the importin-α/β pathway, through a direct interaction with importin-α isoforms. Here we provide a combined nuclear magnetic resonance and small-angle X-ray scattering (NMR/SAXS) analysis to describe the dynamics of the interaction between influenza B NP and the human importin-α. The NP of influenza B does not have a single NLS nor a bipartite NLS but our results suggest that the tail harbors several adjacent NLS sequences, located between residues 30 and 71.

On the validity of the dispersion model of hepatic drug elimination when intravascular transit time densities are long-tailed.

PubMed

Weiss, M; Stedtler, C; Roberts, M S

1997-09-01

The dispersion model with mixed boundary conditions uses a single parameter, the dispersion number, to describe the hepatic elimination of xenobiotics and endogenous substances. An implicit a priori assumption of the model is that the transit time density of intravascular indicators is approximately by an inverse Gaussian distribution. This approximation is limited in that the model poorly describes the tail part of the hepatic outflow curves of vascular indicators. A sum of two inverse Gaussian functions is proposed as an alternative, more flexible empirical model for transit time densities of vascular references. This model suggests that a more accurate description of the tail portion of vascular reference curves yields an elimination rate constant (or intrinsic clearance) which is 40% less than predicted by the dispersion model with mixed boundary conditions. The results emphasize the need to accurately describe outflow curves in using them as a basis for determining pharmacokinetic parameters using hepatic elimination models.

Experimental study of main rotor tip geometry and tail rotor interactions in hover. Volume 2: Run log and tabulated data

NASA Technical Reports Server (NTRS)

Balch, D. T.; Lombardi, J.

1985-01-01

A model scale hover test was conducted in the Sikorsky Aircraft Model Rotor hover Facility to identify and quantify the impact of the tail rotor on the demonstrated advantages of advanced geometry tip configurations. The existence of mutual interference between hovering main rotor and a tail rotor was acknowledged in the test. The test was conducted using the Basic Model Test Rig and two scaled main rotor systems, one representing a 1/5.727 scale UH-60A BLACK HAWK and the others a 1/4.71 scale S-76. Eight alternate rotor tip configurations were tested, 3 on the BLACK HAWK rotor and 6 on the S-76 rotor. Four of these tips were then selected for testing in close proximity to an operating tail rotor (operating in both tractor and pusher modes) to determine if the performance advantages that could be obtained from the use of advanced geometry tips in a main rotor only environment would still exist in the more complex flow field involving a tail rotor. This volume contains the test run log and tabulated data.

An analysis of the effects of aeroelasticity on static longitudinal stability and control of a swept-back-wing airplane

NASA Technical Reports Server (NTRS)

Skoog, Richard B

1951-01-01

A theoretical analysis of the effects of aeroelasticity on the stick-fixed static longitudinal stability and elevator angle required for balance of an airplane is presented together with calculated effects for a swept-wing bomber of relatively high flexibility. Although large changes in stability due to certain parameters are indicated for the example airplane, the over-all stability change after considering all parameters was quite small, compared to the individual effects, due to the counterbalancing of wing and tail contributions. The effect of flexibility on longitudinal control for the example airplane was found to be of little real importance.

Wind-tunnel Investigation of End-plate Effects of Horizontal Tails on a Vertical Tail Compared with Available Theory

NASA Technical Reports Server (NTRS)

Murray, Harry E

1946-01-01

A vertical-tail model with stub fuselage was tested in combination with various simulated horizontal tails to determine the effect of horizontal-tail span and location on the aerodynamic characteristics of the vertical tail. Available theoretical data on end-plate effects were collected and presented in the form most suitable for design purposes. Reasonable agreement was obtained between the measured and theoretical end-plate effects of horizontal tails on vertical tails, and the data indicated that the end-plate effect was determined more by the location of the horizontal tail than by the span of the horizontal tail. The horizontal tail gave most end-plate effect when located near either tip of the vertical tail and, when located near the base of the vertical tail, the end-plate effect was increased by moving the horizontal tail rearward.

Epigenetics Europe conference. Munich, Germany, 8-9 September 2011.

PubMed

Jeltsch, Albert

2011-12-01

At the Epigenetics Europe conference in Munich, Germany, held on 8-9 September 2011, 19 speakers from different European countries were presenting novel data and concepts on molecular epigenetics. The talks were mainly focused on questions of the generation, maintenance, flexibility and erasure of DNA methylation patterns in context of other epigenetic signals like histone tail modifications and ncRNAs.

Multivariable Techniques for High-Speed Research Flight Control Systems

NASA Technical Reports Server (NTRS)

Newman, Brett A.

1999-01-01

This report describes the activities and findings conducted under contract with NASA Langley Research Center. Subject matter is the investigation of suitable multivariable flight control design methodologies and solutions for large, flexible high-speed vehicles. Specifically, methodologies are to address the inner control loops used for stabilization and augmentation of a highly coupled airframe system possibly involving rigid-body motion, structural vibrations, unsteady aerodynamics, and actuator dynamics. Design and analysis techniques considered in this body of work are both conventional-based and contemporary-based, and the vehicle of interest is the High-Speed Civil Transport (HSCT). Major findings include: (1) control architectures based on aft tail only are not well suited for highly flexible, high-speed vehicles, (2) theoretical underpinnings of the Wykes structural mode control logic is based on several assumptions concerning vehicle dynamic characteristics, and if not satisfied, the control logic can break down leading to mode destabilization, (3) two-loop control architectures that utilize small forward vanes with the aft tail provide highly attractive and feasible solutions to the longitudinal axis control challenges, and (4) closed-loop simulation sizing analyses indicate the baseline vane model utilized in this report is most likely oversized for normal loading conditions.

Weighted Kolmogorov-Smirnov test: accounting for the tails.

PubMed

Chicheportiche, Rémy; Bouchaud, Jean-Philippe

2012-10-01

Accurate goodness-of-fit tests for the extreme tails of empirical distributions is a very important issue, relevant in many contexts, including geophysics, insurance, and finance. We have derived exact asymptotic results for a generalization of the large-sample Kolmogorov-Smirnov test, well suited to testing these extreme tails. In passing, we have rederived and made more precise the approximate limit solutions found originally in unrelated fields, first in [L. Turban, J. Phys. A 25, 127 (1992)] and later in [P. L. Krapivsky and S. Redner, Am. J. Phys. 64, 546 (1996)].

The results of a low speed wind tunnel test to investigate the effects of installing refan JT8D engines on the McDonnell Douglas DC-9-30

NASA Technical Reports Server (NTRS)

Chrisenberry, H. E.; Doss, P. G.; Kressly, A. E.; Prichard, R. D.; Thorndike, C. S.

1973-01-01

A low speed wind tunnel test was conducted to assess the effects of the larger JT8D refan nacelles on the stability and control characteristics of the DC-9-30, with emphasis on the deep stall regime. Deep stall pitching moment and elevator hinge moment data, and low angle of attack tail-on and tail-off pitching moment data are presented. The refan nacelle was tested in conjunction with various pylons of reduced span relative to the production DC-9-30 pylon. Also, a horizontal tail that was larger than the production tail was tested. The data show that the refan installation has a small detrimental effect on the DC-9-30 deep stall recovery capability, that recovery characteristics are essentially independent of pylon span, and that the larger horizontal tail significantly increases recovery margins. The deep stall characteristics with the refan installation, within the range of pylon spans tested, are acceptable with no additional design changes anticipated.

Effect of Tail Dihedral on Lateral Control Effectiveness at High Subsonic Speeds of Differentially Deflected Horizontal-Tail Surfaces on a Configuration having a Thin Highly Tapered Wing

NASA Technical Reports Server (NTRS)

Fournier, Paul G.

1959-01-01

Tests have been conducted in the Langley high-speed 7- by 10-foot tunnel to determine the effect of tail dihedral on lateral control effectiveness of a complete-model configuration having differentially deflected horizontal-tail surfaces. Limited tests were made to determine the lateral characteristics as well as the longitudinal characteristics in sideslip. The wing had an aspect ratio of 3, a taper ratio of 0.14, 28.80 deg sweep of the quarter-chord line with zero sweep at the 80-percent-chord line, and NACA 65A004 airfoil sections. The test Mach number range extended from 0.60 to 0.92. There are only small variations in the roll effectiveness parameter C(sub iota delta) with negative tail dihedral angle. The tail size used on the test model, however, is perhaps inadequate for providing the roll rates specified by current military requirements at subsonic speeds. The lateral aerodynamic characteristics were essentially constant throughout the range of sideslip angle from 12 deg to -12 deg. A general increase in yawing moment was noted with increased negative dihedral throughout the Mach number range.

Application of boron/epoxy reinforced aluminum stringers and boron/epoxy skid gear for the CH54B helicopter tail cone. Phase 2: Fabrication, inspection and flight test

NASA Technical Reports Server (NTRS)

Welge, R. T.

1972-01-01

A CH-54B Skycrane helicopter was fabricated with boron/epoxy reinforced stringers in the tail cone and boron/epoxy tubes in the tail skid. The fabrication of the tail cone was made with conventional tooling, production shop personnel, and no major problems. The flight test program includes a stress and vibration survey using strain gages and vibration transducers located in critical areas. The program to inspect and monitor the reliability of the components is discussed.

Strongly Coupled Fluid-Body Dynamics in the Immersed Boundary Projection Method

NASA Astrophysics Data System (ADS)

Wang, Chengjie; Eldredge, Jeff D.

2014-11-01

A computational algorithm is developed to simulate dynamically coupled interaction between fluid and rigid bodies. The basic computational framework is built upon a multi-domain immersed boundary method library, whirl, developed in previous work. In this library, the Navier-Stokes equations for incompressible flow are solved on a uniform Cartesian grid by the vorticity-based immersed boundary projection method of Colonius and Taira. A solver for the dynamics of rigid-body systems is also included. The fluid and rigid-body solvers are strongly coupled with an iterative approach based on the block Gauss-Seidel method. Interfacial force, with its intimate connection with the Lagrange multipliers used in the fluid solver, is used as the primary iteration variable. Relaxation, developed from a stability analysis of the iterative scheme, is used to achieve convergence in only 2-4 iterations per time step. Several two- and three-dimensional numerical tests are conducted to validate and demonstrate the method, including flapping of flexible wings, self-excited oscillations of a system of linked plates and three-dimensional propulsion of flexible fluked tail. This work has been supported by AFOSR, under Award FA9550-11-1-0098.

Rodent models of depression: forced swim and tail suspension behavioral despair tests in rats and mice.

PubMed

Castagné, Vincent; Moser, Paul; Roux, Sylvain; Porsolt, Roger D

2011-04-01

The development of antidepressants requires simple rodent behavioral tests for initial screening before undertaking more complex preclinical tests and clinical evaluation. Presented in the unit are two widely used screening tests used for antidepressants, the forced swim (also termed behavioral despair) test in the rat and mouse, and the tail suspension test in the mouse. These tests have good predictive validity and allow rapid and economical detection of substances with potential antidepressant-like activity. The behavioral despair and the tail suspension tests are based on the same principle: measurement of the duration of immobility when rodents are exposed to an inescapable situation. The majority of clinically used antidepressants decrease the duration of immobility. Antidepressants also increase the latency to immobility, and this additional measure can increase the sensitivity of the behavioral despair test in the mouse for certain classes of antidepressant. Testing of new substances in the behavioral despair and tail suspension tests allows a simple assessment of their potential antidepressant activity by the measurement of their effect on immobility. © 2011 by John Wiley & Sons, Inc.

Experimental parametric studies of transonic T-tail flutter. [wind tunnel tests

NASA Technical Reports Server (NTRS)

Ruhlin, C. L.; Sandford, M. C.

1975-01-01

Wind-tunnel tests of the T-tail of a wide-body jet airplane were made at Mach numbers up to 1.02. The model consisted of a 1/13-size scaled version of the T-tail, fuselage, and inboard wing of the airplane. Two interchangeable T-tails were tested, one with design stiffness for flutter-clearance studies and one with reduced stiffness for flutter-trend studies. Transonic antisymmetric-flutter boundaries were determined for the models with variations in: (1) fin-spar stiffness, (2) stabilizer dihedral angle (-5 deg and 0 deg), (3) wing and forward-fuselage shape, and (4) nose shape of the fin-stabilizer juncture. A transonic symmetric-flutter boundary and flutter trends were established for variations in stabilizer pitch stiffness. Photographs of the test configurations are shown.

Stability and control characteristics of an airplane model having a 45.1 degree swept-back wing with aspect ratio 2.50 and taper ratio 0.42 and a 42.8 degree swept-back horizontal tail with aspect ratio 3.87 and taper ratio 0.49

NASA Technical Reports Server (NTRS)

Schuldenfrei, Marvin; Comisarow, Paul; Goodson, Kenneth W

1947-01-01

Tests were made of an airplane model having a 45.1 degree swept-back wing with aspect ratio 2.50 and taper ratio 0.42 and a 42.8 degree swept-back horizontal tail with aspect ratio 3.87 and taper ratio 0.49 to determine its low-speed stability and control characteristics. The test Reynolds number was 2.87 x 10(6) based on a mean aerodynamic chord of 2.47 feet except for some of the aileron tests which were made at a Reynolds number of 2.05 x 10(6). With the horizontal tail located near the fuselage juncture on the vertical tail, model results indicated static longitudinal instability above a lift coefficient that was 0.15 below the lift coefficient at which stall occurred. Static longitudinal stability, however, was manifested throughout the life range with the horizontal tail located near the top of the vertical tail. The use of 10 degrees negative dihedral on the wing had little effect on the static longitudinal stability characteristics. Preliminary tests of the complete model revealed an undesirable flat spot in the yawing-moment curves at low angles of attack, the directional stability being neutral for yaw angles of plus-or-minus 2 degrees. This undesirable characteristic was improved by replacing the thick original vertical tail with a thin vertical tail and by flattening the top of the dorsal fairing.

[Single cell gel electrophoresis of a magnesium alloy coated with beta-tricalcium phosphate].

PubMed

Hao, Yu-quan; Tan, Li-li; Yan, Ting-ting; Yan, Xiu-lin; Yang, Ke; Ai, Hong-jun

2009-10-01

To evaluate the genotoxicity of a magnesium alloy coated with beta-tricalcium phosphate (beta-TCP). Four groups were designed. In the first group, AZ31B magnesium alloy surface was coated with beta-TCP using chemical bath deposition, and in the second group magnesium alloy was tested. The other two groups were negative control (pure titanium) and positive control groups (0.5 mg/L bleomycin). Single cell gel electrophoresis was adopted to investigate genotoxicity of the alloy samples in different groups, and 60 cells from each group were analysed. Tail moment and tail DNA percentage were used as reliable indicators to show DNA damage in lymphocytes induced by every testing sample. Student-Newman-Keuls (SNK) test was used to compare results from 4 groups. There were no significant differences in tail moment and tail DNA percentage between magnesium alloy group [(0.52 +/- 0.12), (6.82 +/- 1.81)%] and magnesium alloy coated with beta-TCP group [(0.51 +/- 0.12), (6.89 +/- 1.93)%, P > 0.05]. Tail moment and tail DNA percentage in negative group were (0.47 +/- 0.14) and (6.29 +/- 1.64)%, and tail moment and tail DNA percentage in positive group were (5.17 +/- 1.23) and (22.09 +/- 4.51)%. No significant increase was found in DNA damage in lymphocytes induced by magnesium alloy coated with beta-TCP.

Comparison of Vee-Type and Conventional Tail Surfaces in Combination with Fuselage and Wing in the Variable-Density Tunnel

NASA Technical Reports Server (NTRS)

Greenberg, Harry

1941-01-01

The pitching and the yawing moments of a vee-type and a conventional type of tail surface were measured. The tests were made in the presence of a fuselage and a wing-fuselage combination in such a way as to determine the moments contributed by the tail surfaces. The results showed that the vee-type tail tested, with a dihedral angle of 35.3 deg, was about 71 percent as effective in pitch as the conventional tail and had a yawing-moment to pitching-moment ratio of 0.3. The conventional tail, the panels of which were all congruent to those of the vee-type tail, had a yawing-moment to pitching-moment ratio of 0.48. These ratios are in fair agreement with values calculated by methods shown in this and previous reports. The values of the measured moments were reduced from 15 to 25 percent of the calculated value by fuselage interference.

Transiently disordered tails accelerate folding of globular proteins.

PubMed

Mallik, Saurav; Ray, Tanaya; Kundu, Sudip

2017-07-01

Numerous biological proteins exhibit intrinsic disorder at their termini, which are associated with multifarious functional roles. Here, we show the surprising result that an increased percentage of terminal short transiently disordered regions with enhanced flexibility (TstDREF) is associated with accelerated folding rates of globular proteins. Evolutionary conservation of predicted disorder at TstDREFs and drastic alteration of folding rates upon point-mutations suggest critical regulatory role(s) of TstDREFs in shaping the folding kinetics. TstDREFs are associated with long-range intramolecular interactions and the percentage of native secondary structural elements physically contacted by TstDREFs exhibit another surprising positive correlation with folding kinetics. These results allow us to infer probable molecular mechanisms behind the TstDREF-mediated regulation of folding kinetics that challenge protein biochemists to assess by direct experimental testing. © 2017 Federation of European Biochemical Societies.

Mechanical and electrical performance characterization of partial mock-up of the ITER PF6 coil tail

NASA Astrophysics Data System (ADS)

Zhang, Z.; Song, Y.; Wu, H.; Zhang, M.; Xie, Y.; Hu, B.; Liu, F.; Shen, G.; Wu, W.; Lu, K.; Wei, J.; Bilbao, M.; Peñate, J.; Readman, P.; Sborchia, C.; Valente, P.; Smith, K.

2017-12-01

International Thermonuclear Experimental Reactor (ITER) is a full superconducting coil tokamak. The tail is an important component of Poloidal Field (PF) coil, of which the main functions are to provide the electrical isolation and transfer the longitudinal load from the last turn to the last-but-one turn. The paper focuses on an optimized mechanical structure of PF6 coil tail, which is made up of two main parts. One was welded to the last turn and the other was welded to the last-but-one turn. Both of them were connected by the mechanical coupling. The electrical isolation between the two parts was maintained by a strap made of insulating composite. In addition, as the PF6 coil is operated under the cyclic electromagnetic load during the tokamak operation, the fatigue property of the tail should be assessed and qualified at low temperature. Moreover, taking into consideration the complexity of the insulation winding process which is performed in a confined space, the wrapping process of the insulation needs to be established. Meanwhile, the high voltage (HV) tests of the tail insulation, including the direct current (DC) and alternating current (AC) tests, need to be assessed before and after the fatigue test. In this paper, a fully bonded PF6 coil tail partial mock-up (not including the weld of the tail to the last conductor turn) was designed and manufactured by simulating the actual manufacturing processes. In addition, the fatigue tests on the sample were carried out at 77 K, and the results showed the sample had good and stable fatigue properties at cryogenic temperature. The HV tests before and after the fatigue test, also including the final 30 kV breakdown DC test after the fatigue test, were carried out. The test results satisfied the requirements of ITER and were discussed in depth. Finally, the sample was destructively inspected to validate the integrity of the insulation by mechanical cross sectioning, and no voids and cracks were observed. Therefore it can be verified from the test results that the designed PF6 coil tail has good comprehensive properties, which can be applied to the formal production of the PF6 coil.

Determination of the reaction rate coefficient of sulphide mine tailings deposited under water.

PubMed

Awoh, Akué Sylvette; Mbonimpa, Mamert; Bussière, Bruno

2013-10-15

The efficiency of a water cover to limit dissolved oxygen (DO) availability to underlying acid-generating mine tailings can be assessed by calculating the DO flux at the tailings-water interface. Fick's equations, which are generally used to calculate this flux, require knowing the effective DO diffusion coefficient (Dw) and the reaction (consumption) rate coefficient (Kr) of the tailings, or the DO concentration profile. Whereas Dw can be accurately estimated, few studies have measured the parameter Kr for submerged sulphide tailings. The objective of this study was to determine Kr for underwater sulphide tailings in a laboratory experiment. Samples of sulphide mine tailings (an approximately 6 cm layer) were placed in a cell under a water cover (approximately 2 cm) maintained at constant DO concentration. Two tailings were studied: TA1 with high sulphide content (83% pyrite) and TA2 with low sulphide content (2.8% pyrite). DO concentration was measured with a microelectrode at various depths above and below the tailings-water interface at 1 mm intervals. Results indicate that steady-state condition was rapidly attained. As expected, a diffusive boundary layer (DBL) was observed in all cases. An iterative back-calculation process using the numerical code POLLUTEv6 and taking the DBL into account provided the Kr values used to match calculated and experimental concentration profiles. Kr obtained for tailings TA1 and TA2 was about 80 d(-1) and 6.5 d(-1), respectively. For comparison purposes, Kr obtained from cell tests on tailings TA1 was lower than Kr calculated from the sulphate production rate obtained from shake-flask tests. Steady-state DO flux at the water-tailings interface was then calculated with POLLUTEv6 using tailings characteristics Dw and Kr. For the tested conditions, DO flux ranged from 608 to 758 mg O2/m(2)/d for tailings TA1 and from 177 to 221 mg O2/m(2)/d for tailings TA2. The impact of placing a protective layer of inert material over the tailings was also investigated for tailings TA1 (with high sulphide content). A protective layer of only 5 cm reduced the DO flux into the tailings at about 5 mg/m(2)/d, compared to 608 mg O2/m(2)/d without a protective layer, or an approximately 99% reduction in flux. Copyright © 2013 Elsevier Ltd. All rights reserved.

Flight Investigation of Effect of Various Vertical-Tail Modifications on the Directional Stability and Control Characteristics of the P-63A-1 Airplane (AAF No. 42-68889)

NASA Technical Reports Server (NTRS)

Johnson, Harold I.

1946-01-01

Because the results of preliminary flight tests had indicated. the P-63A-1 airplane possessed insufficient directional stability, the NACA and the manufacturer (Bell Aircraft Corporation) suggested three vertical-tail modifications to remedy the deficiencies in the directional characteristics. These modifications included an enlarged vertical tail formed by adding a tip extension to the original vertical tail, a large sharp-edge ventral fin, and a small dorsal fin. The enlarged vertical tail involved only a slight increase in total vertical-tail area from 23.73 to 26.58 square feet but a relatively much larger increase in geometric aspect ratio from 1.24 to 1.73 based on height and area above the horizontal tail. At the request of the Air Material Command, Army Air Forces, flight tests were made to determine the effect of these modifications and of some combinations of these modifications on the directional stability and control characteristics of the airplane, In all, six different vertical-tail. configurations were investigated to determine the lateral and directional oscillation characteristics of the airplane, the sideslip characteristics, the yaw due to ailerons in rudder-fixed rolls from turns and pull-outs, the trim changes due to speed changes; and the trim changes due to power changes. Results of the tests showed that the enlarged vertical tail approximately doubled the directional stability of the airplane and that the pilots considered the directional stability provided by the enlarged vertical tail to be satisfactory. Calculations based on sideslip data obtained at an indicated airspeed of 300 miles per hour showed that the directional stability of the airplane with the original vertical tail corresponded to a value of 0(sub n beta) of -0.00056 whereas for the enlarged vertical tail the estimated va1ue of C(sub n beta) was -0.00130, The ventral fin was found to increase by a moderate amount the directional stability of the airplane with the original vertical tail for smal1 sides1ip angles at low speeds but little consistent change in directional stability was effected by the ventral fin at higher speeds, The effectiveness of the ventral fin was generally much less when used with the enlarged vertical tail than when used with the original vertical tail. The ventral and dorsal fins were found to be very effective in eliminating rudder-force reversals which occurred in low-speed, high-engine-power, sideslipped conditions of flight . Sideslip tests at two altitudes for approximately the sane engine power and indicated airspeed showed that a small decrease in static directional stability occurred with increasing altitude and this decrease in stability was attributed to the increased propeller blade angles required at high altitudes. The variations of rudder pedal force with indicated airspeed using normal rated power and a constant rudder tab setting through the speed range were desirably small for all the configurations tested. The rudder pedal force changed by about 50 pounds for a power change from engine idling power, to normal rated power and this pedal force change was largely independent of airspeed or of vertical-tail configuration for the various configurations tested.

Effect of vertical-tail location on the aerodynamic characteristics at subsonic speeds of a close-coupled canard configuration

NASA Technical Reports Server (NTRS)

Huffman, J. K.

1975-01-01

The effects were studied of various vertical-tail configurations on the longitudinal and lateral directional-stability characteristics of a general research fighter model utilizing wing-body-canard. The study indicates that the addition of the high canard resulted in an increase in total lift at angles of attack above 4 deg with a maximum lift coefficient about twice as large as that for the wing-body configuration. For the wing-body (canard off) configuration, the center-line vertical tail indicates positive vertical-tail effectiveness throughout the test angle-of-attack range; however, for this configuration none of the wing-mounted vertical-tail locations tested resulted in a positive directional-stability increment at the higher angles of attack. For the wing-body-canard configuration several outboard locations of the wing-mounted vertical tails were found.

Evaluation of the grass mixture (Faestuca Rubra, Cynodon Dactylon, Lolium Multiflorum and Pennisetum sp.) as Sb phyto-stabilizer in tailings and Sb-rich soils.

NASA Astrophysics Data System (ADS)

Aurora Armienta, M.; Beltrán-Villavicencio, Margarita; Ruiz-Villalobos, Carlos E.; Labastida, Israel; Ceniceros, Nora; Cruz, Olivia; Aguayo, Alejandra

2017-04-01

Green house experiments were carried out to evaluate the growth and Sb assimilation of a grass assemblage: Faestuca Rubra, Cynodon Dactylon, Lolium Multiflorum and Pennisetum sp, in tailings and Sb-rich soils. Tailings and soil samples were obtained at the Mexican historical mining zone of Zimapán, Central México. More than 6 tailings impoundments are located at the town outskirts and constitute a contamination source from windblown and waterborne deposit on soils, besides acid mine drainage. Four substrates were used in the experiments: 100% tailings, 20% tailings + 80% soil, 50% tailings + 50% soil , and a soil sample far from tailings as a background. Concentrations of Sb ranged from 310 mg/kg to 413 mg/kg in tailings. A pH of 7.43, 1.27% organic matter, and high concentrations of N, K and P indicated adequate conditions for plant growth. The grass assemblage was raised during 21 days as indicated by OECD (Organisation for Economic Co-operation and Development) Guideline 208 Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test. The highest Sb concentrations were measured in plants grown on tailings with 139 mg/kg in the aerial part and 883 mg/kg in roots. Concentrations of Sb decreased as the proportion of tailings diminished with 22.1 mg/kg in the aerial part and 10 mg/kg in roots corresponding to the plants grown in the 20 % tailings + 80% soil . Bioaccumulation (BAC) and bioconcentration factors (BF) of plants grown on tailings (BAC= 0.42, BCF=3.93) indicated their suitability as a phyto-stabilization option. The grass mixture may be thus applied to control windblown particulate tailings taking advantage to their tolerance to high Sb levels.

How far in-silico computing meets real experiments. A study on the structure and dynamics of spin labeled vinculin tail protein by molecular dynamics simulations and EPR spectroscopy

PubMed Central

2013-01-01

Background Investigation of conformational changes in a protein is a prerequisite to understand its biological function. To explore these conformational changes in proteins we developed a strategy with the combination of molecular dynamics (MD) simulations and electron paramagnetic resonance (EPR) spectroscopy. The major goal of this work is to investigate how far computer simulations can meet the experiments. Methods Vinculin tail protein is chosen as a model system as conformational changes within the vinculin protein are believed to be important for its biological function at the sites of cell adhesion. MD simulations were performed on vinculin tail protein both in water and in vacuo environments. EPR experimental data is compared with those of the simulated data for corresponding spin label positions. Results The calculated EPR spectra from MD simulations trajectories of selected spin labelled positions are comparable to experimental EPR spectra. The results show that the information contained in the spin label mobility provides a powerful means of mapping protein folds and their conformational changes. Conclusions The results suggest the localization of dynamic and flexible regions of the vinculin tail protein. This study shows MD simulations can be used as a complementary tool to interpret experimental EPR data. PMID:23445506

A mechanism of thrust enhancement on a heaving plate due to flexibility at moderately low Reynolds numbers

NASA Astrophysics Data System (ADS)

Lin, Yung-Sheng; Tzeng, Yau-Ting; Chang, Chien-Cheng; Chu, Chin-Chou

2017-11-01

A numerical study is conducted to investigate the force mechanisms for a 3D heaving flexible plate from the perspective of a diagnostic force element analysis (Chang 1992). The problem is relevant to a simplified flapping fish-tail with the front edge held fixed in space. The flow is assumed to be laminar with the Reynolds numbers fixed at Re =200 or 500, and the Strouhal number St ranging from 0.1 to 0.6, and the flexure amplitude of the plate a0 for 0.1 to 0.25 (dimensionless). It is shown that heaving, whilst increasing thrust generation, also reduces the frictional drag, yet the flexibility promotes thrust generation at the expense of accruing more frictional drag. In the literature, the thrust exerted on the tail-mimicking plate is largely credited to the vortices in the wake. However, this study performs a regional force analysis to show that the vorticity in the wake region supplies approximately 20-30% of the total thrust, especially in the cases of strong thrust generation. Comparable contributions come also from the regions direct above and below the heaving plate (mainly including the attached vortices) as well as from the two side regions (mainly including the tip vortices) next to the flapping plate. In addition, the potential motion associated with the unsteady flapping and the contribution from the surface vorticity are non-negligible constituent force components. MOST, TAIWAN under Contacts NO. 105-2221-E-002-097-MY3 and NO. 105-2221-E-002 -105 -MY3.

The impact of tail tip amputation and ink tattoo on C57BL/6JBomTac mice.

PubMed

Sørensen, Dorte Bratbo; Stub, Charlotte; Jensen, Henrik Elvang; Ritskes-Hoitinga, Merel; Hjorth, Peter; Ottesen, Jan Lund; Hansen, Axel Kornerup

2007-01-01

Genetic material for polymerase chain reaction (PCR) and Southern blot analysis on transgenic mice is normally obtained by tail biopsy. Additionally, it may be necessary to tattoo the mice, as it is essential to have a good and permanent identification. The aim of this study was to evaluate the effects of amputating the tip of the tail to obtain a biopsy for genetic analysis and of ink tattooing on welfare in C57BL/6J mice, a strain often used as genetic background for transgenes. The behaviour of the animals, fluctuating asymmetry (FA, a measure of developmental instability) and the level of restitution in the remaining part of the tail were evaluated and used for an assessment of the impact of these procedures on the welfare of the animals. One group of mice was marked by tail tattooing at various ages. Another group of mice were tail amputated at 12 or 20 days of age. Body weight and FA were followed, and at the end of the experiment, the level of fear/anxiety was assessed using a light-dark box. In the group of tail-amputated animals observation of climbing behaviour and a beam walking test for balance was performed. Seven weeks after tail amputation, the animals were euthanized. The remaining part of the tail was evaluated histopathologically. Body weight, behaviour in the light-dark box and balance test results were not influenced by tail amputation or tattooing. FA was only transiently increased by tattooing. Climbing behaviour was reduced just after tail amputation at 20 days of age. No signs of neuromas were found in the amputated tails, but seven weeks after amputation a significant number of mice did not have fully regenerated glandular tissue and hair follicles in the tail. It is concluded that both tail amputation and tail tattooing seem to have minor short-term negative effects on welfare and that the tissues on the tail probably do not regenerate fully after amputation.

Tail function during arboreal quadrupedalism in squirrel monkeys (Saimiri boliviensis) and tamarins (Saguinus oedipus).

PubMed

Young, Jesse W; Russo, Gabrielle A; Fellmann, Connie D; Thatikunta, Meena A; Chadwell, Brad A

2015-10-01

The need to maintain stability on narrow branches is often presented as a major selective force shaping primate morphology, with adaptations to facilitate grasping receiving particular attention. The functional importance of a long and mobile tail for maintaining arboreal stability has been comparatively understudied. Tails can facilitate arboreal balance by acting as either static counterbalances or dynamic inertial appendages able to modulate whole-body angular momentum. We investigate associations between tail use and inferred grasping ability in two closely related cebid platyrrhines-cotton-top tamarins (Saguinus oedipus) and black-capped squirrel monkeys (Saimiri boliviensis). Using high-speed videography of captive monkeys moving on 3.2 cm diameter poles, we specifically test the hypothesis that squirrel monkeys (characterized by grasping extremities with long digits) will be less dependent on the tail for balance than tamarins (characterized by claw-like nails, short digits, and a reduced hallux). Tamarins have relatively longer tails than squirrel monkeys, move their tails through greater angular amplitudes, at higher angular velocities, and with greater angular accelerations, suggesting dynamic use of tail to regulate whole-body angular momentum. By contrast, squirrel monkeys generally hold their tails in a comparatively stationary posture and at more depressed angles, suggesting a static counterbalancing mechanism. This study, the first empirical test of functional tradeoffs between grasping ability and tail use in arboreal primates, suggests a critical role for the tail in maintaining stability during arboreal quadrupedalism. Our findings have the potential to inform our functional understanding of tail loss during primate evolution. © 2015 Wiley Periodicals, Inc.

Factors affecting the transformation of a pyritic tailing: scaled-up column tests.

PubMed

García, C; Ballester, A; González, F; Blázquez, M L

2005-02-14

Two different methods for predicting the quality of the water draining from a pyritic tailing are compared; for this, a static test (ABA test) and a kinetic test in large columns were chosen. The different results obtained in the two experimental set-ups show the necessity of being careful in selecting both the adequate predictive method and the conclusions and extrapolations derived from them. The tailing chosen for the weathering tests (previously tested in shake flasks and in small weathering columns) was a pyritic residue produced in a flotation plant of complex polymetallic sulphides (Huelva, Spain). The ABA test was a modification of the conventional ABA test reported in bibliography. The modification consisted in the soft conditions employed in the digestion phase. For column tests, two identical methacrylate columns (150 cm high and 15 cm diameter) were used to study the chemical and microbiological processes controlling the leaching of pyrite. The results obtained in the two tests were very different. The static test predicted a strong potential acidity for the tailing. On the contrary, pH value in the effluents draining from the columns reached values of only 5 units, being the concentration of metals (<600 mg/L) and sulphate ions (<17,000 mg/L) very small and far from the values of a typical acid mine drainage. In consequence, the static test may oversize the potential acidity of the tailing; whereas large columns may be saturated in water, displacing the oxygen and inhibiting the microbial activity necessary to catalyse mineral oxidation.

Design and Development of the Space Shuttle Tail Service Masts

NASA Technical Reports Server (NTRS)

Dandage, S. R.; Herman, N. A.; Godfrey, S. E.; Uda, R. T.

1977-01-01

The results of the tail service masts (TSM) concept verification test are presented along with the resulting impact on prototype design. The design criteria are outlined, and the proposed prototype TSM tests are described.

Hemispheric-scale wind selection facilitates bar-tailed godwit circum-migration of the Pacific

USGS Publications Warehouse

Gill, Robert E.; Douglas, David C.; Handel, Colleen M.; Tibbitts, T. Lee; Hufford, Gary; Piersma, Theunis

2014-01-01

The annual 29 000 km long migration of the bar-tailed godwit, Limosa lapponica baueri, around the Pacific Ocean traverses what is arguably the most complex and seasonally structured atmospheric setting on Earth. Faced with marked variation in wind regimes and storm conditions across oceanic migration corridors, individuals must make critical decisions about when and where to fly during nonstop flights of a week's duration or longer. At a minimum, their decisions will affect wind profitability and thus reduce energetic costs of migration; in the extreme, poor decisions or unpredictable weather events will risk survival. We used satellite telemetry to track the annual migration of 24 bar-tailed godwits and analysed their flight performance relative to wind conditions during three major migration legs between nonbreeding grounds in New Zealand and breeding grounds in Alaska. Because flight altitudes of birds en route were unknown, we modelled flight efficiency at six geopotential heights across each migratory segment. Birds selected departure dates when atmospheric conditions conferred the greatest wind assistance both at departure and throughout their flights. This behaviour suggests that there exists a cognitive mechanism, heretofore unknown among migratory birds, that allows godwits to assess changes in weather conditions that are linked (i.e. teleconnected) across widely separated atmospheric regions. Godwits also showed adaptive flexibility in their response not only to cues related to seasonal changes in macrometeorology, such as spatial shifting of storm tracks and temporal periods of cyclogenesis, but also to cues associated with stochastic events, especially at departure sites. Godwits showed limits to their response behaviours, however, especially relative to rapidly developing stochastic events while en route. We found that flight efficiency depended significantly upon altitude and hypothesize that godwits exhibit further adaptive flexibility by varying flight altitude en route to optimize flight efficiency.

Aerodynamics of an Axisymmetric Missile Concept Having Cruciform Strakes and In-Line Tail Fins From Mach 0.60 to 4.63

NASA Technical Reports Server (NTRS)

Allen, Jerry M.

2005-01-01

An experimental study has been performed to develop a large force and moment aerodynamic data set on a slender axisymmetric missile configuration having cruciform strakes and in-line control tail fins. The data include six-component balance measurements of the configuration aerodynamics and three-component measurements on all four tail fins. The test variables include angle of attack, roll angle, Mach number, model buildup, strake length, nose size, and tail fin deflection angles to provide pitch, yaw, and roll control. Test Mach numbers ranged from 0.60 to 4.63. The entire data set is presented on a CD-ROM that is attached to this paper. The CD-ROM also includes extensive plots of both the six-component configuration data and the three-component tail fin data. Selected samples of these plots are presented in this paper to illustrate the features of the data and to investigate the effects of the test variables.

Aerodynamics of an Axisymmetric Missile Concept Having Cruciform Strakes and In-Line Tail Fins From Mach 0.60 to 4.63, Supplement

NASA Technical Reports Server (NTRS)

Allen, Jerry M.

2005-01-01

An experimental study has been performed to develop a large force and moment aerodynamic data set on a slender axisymmetric missile configuration having cruciform strakes and in-line control tail fins. The data include six-component balance measurements of the configuration aerodynamics and three-component measurements on all four tail fins. The test variables include angle of attack, roll angle, Mach number, model buildup, strake length, nose size, and tail fin deflection angles to provide pitch, yaw, and roll control. Test Mach numbers ranged from 0.60 to 4.63. The entire data set is presented on a CD-ROM that is attached to this paper. The CD-ROM also includes extensive plots of both the six-component configuration data and the three-component tail fin data. Selected samples of these plots are presented in this paper to illustrate the features of the data and to investigate the effects of the test variables.

The analysis of axisymmetric viscoelasticity, time-dependent recovery, and hydration in rat tail intervertebral discs by radial compression test.

PubMed

Lin, Leou-Chyr; Hedman, Thomas P; Wang, Shyu-Jye; Huoh, Michael; Chang, Shih-Youeng

2009-05-01

The goal of this study was to develop a nondestructive radial compression technique and to investigate the viscoelastic behavior of the rat tail disc under repeated radial compression. Rat tail intervertebral disc underwent radial compression relaxation testing and creep testing using a custom-made gravitational creep machine. The axisymmetric viscoelasticity and time-dependent recovery were determined. Different levels of hydration (with or without normal saline spray) were supplied to evaluate the effect of changes in viscoelastic properties. Viscoelasticity was found to be axisymmetric in rat-tail intervertebral discs at four equidistant locations. Complete relaxation recovery was found to take 20 min, whereas creep recovery required 25 min. Hydration was required for obtaining viscoelastic axisymmetry and complete viscoelastic recovery.

Qualification of Bonding Process of Temperature Sensors to Extreme Temperature Deep Space Missions

NASA Technical Reports Server (NTRS)

Ramesham, Rajeshuni; Kitiyakara, Amarit; Redick, Richard; Sunada, Eric T.

2011-01-01

A process has been explored based on the state-of-the-art technology to bond the platinum resistance thermometer (PRT) on to potential aerospace material such as a flat aluminum surface and a flexible copper tube to simulate coaxial cable for the flight applications. Primarily, PRTs were inserted into a metal plated copper braid to avoid stresses on the sensor while attaching the sensor with braid to the base material for long duration deep space missions. Appropriate pretreatment has been implemented in this study to enhance the adhesion of the PRTs to the base material. NuSil product has been chosen in this research to attach PRT to the base materials. The resistance (approx.1.1 k(Omega)) of PRTs has been electrically monitored continuously during the qualification thermal cycling testing from -150 C to +120 C and -100 C to -35 C. The test hardware has been thermal cycled three times the mission life per JPL design principles for JUNO project. No PRT failures were observed during and after the PRT thermal cycling qualification test for extreme temperature environments. However, there were some failures associated with staking of the PRT pig tails as a result of thermal cycling qualification test.

Static strain and vibration characteristics of a metal semimonocoque helicopter tail cone of moderate size

NASA Technical Reports Server (NTRS)

Bielawa, Richard L.; Hefner, Rachel E.; Castagna, Andre

1991-01-01

The results are presented of an analytic and experimental research program involving a Sikorsky S-55 helicopter tail cone directed ultimately to the improved structural analysis of airframe substructures typical of moderate sized helicopters of metal semimonocoque construction. Experimental static strain and dynamic shake-testing measurements are presented. Correlation studies of each of these tests with a PC-based finite element analysis (COSMOS/M) are described. The tests included static loadings at the end of the tail cone supported in the cantilever configuration as well as vibrational shake-testing in both the cantilever and free-free configurations.

Weighted Kolmogorov-Smirnov test: Accounting for the tails

NASA Astrophysics Data System (ADS)

Chicheportiche, Rémy; Bouchaud, Jean-Philippe

2012-10-01

Accurate goodness-of-fit tests for the extreme tails of empirical distributions is a very important issue, relevant in many contexts, including geophysics, insurance, and finance. We have derived exact asymptotic results for a generalization of the large-sample Kolmogorov-Smirnov test, well suited to testing these extreme tails. In passing, we have rederived and made more precise the approximate limit solutions found originally in unrelated fields, first in [L. Turban, J. Phys. A1361-644710.1088/0305-4470/25/3/008 25, 127 (1992)] and later in [P. L. Krapivsky and S. Redner, Am. J. Phys.AJPIAS0002-950510.1119/1.18152 64, 546 (1996)].

Derivation of charts for determining the horizontal tail load variation with any elevator motion

NASA Technical Reports Server (NTRS)

Pearson, Henry A

1943-01-01

The equations relating the wing and tail loads are derived for a unit elevator displacement. These equations are then converted into a nondimensional form and charts are given by which the wing- and tail-load-increment variation may be determined under dynamic conditions for any type of elevator motion and for various degrees of airplane stability. In order to illustrate the use of the charts, several examples are included in which the wing and tail loads are evaluated for a number of types of elevator motion. Methods are given for determining the necessary derivatives from results of wind-tunnel tests when such tests are available.

Location tests for biomarker studies: a comparison using simulations for the two-sample case.

PubMed

Scheinhardt, M O; Ziegler, A

2013-01-01

Gene, protein, or metabolite expression levels are often non-normally distributed, heavy tailed and contain outliers. Standard statistical approaches may fail as location tests in this situation. In three Monte-Carlo simulation studies, we aimed at comparing the type I error levels and empirical power of standard location tests and three adaptive tests [O'Gorman, Can J Stat 1997; 25: 269 -279; Keselman et al., Brit J Math Stat Psychol 2007; 60: 267- 293; Szymczak et al., Stat Med 2013; 32: 524 - 537] for a wide range of distributions. We simulated two-sample scenarios using the g-and-k-distribution family to systematically vary tail length and skewness with identical and varying variability between groups. All tests kept the type I error level when groups did not vary in their variability. The standard non-parametric U-test performed well in all simulated scenarios. It was outperformed by the two non-parametric adaptive methods in case of heavy tails or large skewness. Most tests did not keep the type I error level for skewed data in the case of heterogeneous variances. The standard U-test was a powerful and robust location test for most of the simulated scenarios except for very heavy tailed or heavy skewed data, and it is thus to be recommended except for these cases. The non-parametric adaptive tests were powerful for both normal and non-normal distributions under sample variance homogeneity. But when sample variances differed, they did not keep the type I error level. The parametric adaptive test lacks power for skewed and heavy tailed distributions.

Speciation and characterization of arsenic in Ketza River mine tailings using X-ray absorption spectroscopy.

PubMed

Paktunc, Dogan; Foster, Andrea; Laflamme, Gilles

2003-05-15

Ketza River mine tailings deposited underwater and those exposed near the tailings impoundment contain approximately 4 wt % As. Column-leaching tests indicated the potential for high As releases from the tailings. The tailings are composed dominantly of iron oxyhydroxides, quartz, calcite, dolomite, muscovite, ferric arsenates, and calcium-iron arsenates. Arsenopyrite and pyrite are trace constituents. Chemical compositions of iron oxyhydroxide and arsenate minerals are highly variable. The XANES spectra indicate that arsenic occurs as As(V) in tailings, but air-drying prior to analysis may have oxidized lower-valent As. The EXAFS spectra indicate As-Fe distances of 3.35-3.36 A for the exposed tailings and 3.33-3.35 A for the saturated tailings with coordination numbers of 0.96-1.11 and 0.46-0.64, respectively. The As-Ca interatomic distances ranging from 4.15 to 4.18 A and the coordination numbers of 4.12-4.58 confirm the presence of calcium-iron arsenates in the tailings. These results suggest that ferric arsenates and inner-sphere corner sharing or bidentate-binuclear attachment of arsenate tetrahedra onto iron hydroxide octahedra are the dominant form of As in the tailings. EXAFS spectra indicate that the exposed tailings are richer in arsenate minerals whereas the saturated tailings are dominated by the iron oxyhydroxides, which could help explain the greater release of As from the exposed tailings during leaching tests. It is postulated that the dissolution of ferric arsenates during flow-through experiments caused the high As releases from both types of tailings. Arsenic tied to iron oxyhydroxides as adsorbed species are considered stable; however, iron oxyhydroxides having low Fe/As molar ratios may not be as stable. Continued As releases from the tailings are likely due to dissolution of both ferric and calcium-iron arsenates and desorption of As from high-As bearing iron oxyhydroxides during aging.

Speciation and characterization of arsenic in Ketza River mine tailings using x-ray absorption spectroscopy

USGS Publications Warehouse

Paktunc, D.; Foster, A.; Laflamme, G.

2003-01-01

Ketza River mine tailings deposited underwater and those exposed near the tailings impoundment contain approximately 4 wt % As. Column-leaching tests indicated the potential for high As releases from the tailings. The tailings are composed dominantly of iron oxyhydroxides, quartz, calcite, dolomite, muscovite, ferric arsenates, and calcium-iron arsenates. Arsenopyrite and pyrite are trace constituents. Chemical compositions of iron oxyhydroxide and arsenate minerals are highly variable. The XANES spectra indicate that arsenic occurs as As(V) in tailings, but air-drying prior to analysis may have oxidized lower-valent As. The EXAFS spectra indicate As-Fe distances of 3.35-3.36 A?? for the exposed tailings and 3.33-3.35 A?? for the saturated tailings with coordination numbers of 0.96-1.11 and 0.46-0.64, respectively. The As-Ca interatomic distances ranging from 4.15 to 4.18 A?? and the coordination numbers of 4.12-4.58 confirm the presence of calcium-iron arsenates in the tailings. These results suggest that ferric arsenates and inner-sphere corner sharing or bidentatebinuclear attachment of arsenate tetrahedra onto iron hydroxide octahedra are the dominant form of As in the tailings. EXAFS spectra indicate that the exposed tailings are richer in arsenate minerals whereas the saturated tailings are dominated by the iron oxyhydroxides, which could help explain the greater release of As from the exposed tailings during leaching tests. It is postulated that the dissolution of ferric arsenates during flow-through experiments caused the high As releases from both types of tailings. Arsenic tied to iron oxyhydroxides as adsorbed species are considered stable; however, iron oxyhydroxides having low Fe/As molar ratios may not be as stable. Continued As releases from the tailings are likely due to dissolution of both ferric and calcium-iron arsenates and desorption of As from high-As bearing iron oxyhydroxides during aging.

The antinociceptive effect and mechanisms of action of pregabalin in mice.

PubMed

Kaygisiz, Bilgin; Kilic, Fatma Sultan; Senguleroglu, Nuri; Baydemir, Canan; Erol, Kevser

2015-02-01

Pregabalin, a potent anticonvulsant agent, is used in treatment-resistant epileptic patients. It is reported that pregabalin also has analgesic effect in different pain syndromes. However, there is limited data on its antinociceptive mechanisms of action. We aimed to investigate the central and peripheral antinociceptive effects of pregabalin and the contribution of nitrergic, serotonergic, and opioidergic pathways in mice. We used tail flick, tail clip and hot plate tests to investigate the central antinociceptive effects and acetic acid-induced writhing test to assess peripheral antinociceptive effects of pregabalin (10, 30, 100mg/kg). We also combined pregabalin (100mg/kg) with, a nonspecific nitric oxide synthase inhibitor l-NAME (100mg/kg), a serotonin receptor antagonist cyproheptadine (50 μg/kg), and an opioid receptor antagonist naloxone (1mg/kg). Pregabalin 30 mg/kg enhanced the percentage of maximal possible effect (% MPE) in tail flick test. Pregabalin 100mg/kg significantly increased % MPE in tail clip and tail flick tests and decreased the number of writhings. Pregabalin made no significant alteration in hot plate test at all doses. The combined use of pregabalin 100mg/kg with l-NAME, cyproheptadine, and naloxone showed that % MPE was reduced only in the combination of pregabalin with naloxone and solely in tail clip test while no significant difference was observed in writhing test. We suggest that pregabalin (30 and 100mg/kg) presents central spinal but not central supraspinal antinociceptive effect and pregabalin 100mg/kg shows peripheral antinociceptive effect. The opioidergic pathway seems to mediate the central spinal antinociceptive effect of pregabalin while nitrergic and serotonergic pathways are not involved. Copyright © 2014 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

Anomalous transport in fracture networks: field scale experiments and modelling

NASA Astrophysics Data System (ADS)

Kang, P. K.; Le Borgne, T.; Bour, O.; Dentz, M.; Juanes, R.

2012-12-01

Anomalous transport is widely observed in different settings and scales of transport through porous and fractured geologic media. A common signature of anomalous transport is the late-time power law tailing in breakthrough curves (BTCs) during tracer tests. Various conceptual models of anomalous transport have been proposed, including multirate mass transfer, continuous time random walk, and stream tube models. Since different conceptual models can produce equally good fits to a single BTC, tracer test interpretation has been plagued with ambiguity. Here, we propose to resolve such ambiguity by analyzing BTCs obtained from both convergent and push-pull flow configurations at two different fracture planes. We conducted field tracer tests in a fractured granite formation close to Ploemeur, France. We observe that BTC tailing depends on the flow configuration and the injection fracture. Specifically the tailing disappears under push-pull geometry, and when we injected at a fracture with high flux (Figure 1). This indicates that for this fractured granite, BTC tailing is controlled by heterogeneous advection and not by matrix diffusion. To explain the change in tailing behavior for different flow configurations, we employ a simple lattice network model with heterogeneous conductivity distribution. The model assigns random conductivities to the fractures and solves the Darcy equation for an incompressible fluid, enforcing mass conservation at fracture intersections. The mass conservation constraint yields a correlated random flow through the fracture system. We investigate whether BTC tailing can be explained by the spatial distribution of preferential flow paths and stagnation zones, which is controlled by the conductivity variance and correlation length. By combining the results from the field tests and numerical modeling, we show that the reversibility of spreading is a key mechanism that needs to be captured. We also demonstrate the dominant role of the injection fracture on the tailing behavior: where we inject makes the difference in the tailing. Blue line is a BTC with injection into a slow velocity zone under convergent flow configuration. The late-time tailing observed for the convergent test diminished for push-pull experiment performed in the same zone(red line). Black line is a BTC with injection into a high velocity zone under convergent flow configuration. Insets: illustration of convergent and push-pull tracer tests using a double packer system.

Antidepressant-like effect of rutin isolated from the ethanolic extract from Schinus molle L. in mice: evidence for the involvement of the serotonergic and noradrenergic systems.

PubMed

Machado, Daniele G; Bettio, Luis E B; Cunha, Mauricio P; Santos, Adair R S; Pizzolatti, Moacir G; Brighente, Inês M C; Rodrigues, Ana Lúcia S

2008-06-10

We have recently shown that the hexanic extract from leaves of Schinus molle produces antidepressant-like effects in the tail suspension test in mice. This study investigated the antidepressant-like effect of the ethanolic extract from aerial part of S. molle in the forced swimming test and tail suspension test in mice, two predictive models of depression. Moreover, we investigated the antidepressant potential of rutin, a flavonoid isolated from the ethanolic extract of this plant and the influence of the pretreatment with the inhibitors of serotonin or noradrenaline synthesis, p-chlorophenylalanine methyl ester (PCPA) and alpha-methyl-p-tyrosine (AMPT), respectively in the antidepressant-like effect of this flavonoid. The administration of the ethanolic extract produced a reduction in the immobility time in the tail suspension test (dose range 600-1000 mg/kg, p.o.), but not in the forced swimming test. It also produced a reduction in the ambulation in the open-field test in mice not previously habituated to the arena, but no effect in the locomotor activity in mice previously habituated to the open-field. The administration of rutin reduced the immobility time in the tail suspension test (0.3-3 mg/kg, p.o.), but not in the forced swimming test, without producing alteration in the locomotor activity. In addition, pretreatment of mice with PCPA (100 mg/kg, i.p., for 4 consecutive days) or AMPT (100 mg/kg, i.p.) prevented the anti-immobility effect of rutin (0.3 mg/kg, p.o.) in the tail suspension test. The results firstly indicated the antidepressant-like effect of the ethanolic extract of S. molle in the tail suspension test may be dependent on the presence of rutin that likely exerts its antidepressant-like effect by increasing the availability of serotonin and noradrenaline in the synaptic cleft.

Review and Evaluation of Reservoir Management Strategies for Harmful Algal Blooms

DTIC Science & Technology

2017-07-28

report is to review and evaluate available information regarding reservoir operation strategies for management of harmful algal ERDC/EL TR-17-11 2...health. Resource managers are challenged to consider de- tailed information such as algal growth patterns, environmental conditions, dominant...need to be specifically tailored to the situa- tion at hand and managers must be flexible in their approach, taking into consideration new information

An Investigation of the Elements which Contribute to Statical and Dynamical Stability, and of the Effects of Variation in Those Elements

NASA Technical Reports Server (NTRS)

Klemin, Alexander; Warner, Edward P; Denkinger, George M

1918-01-01

Part 1 gives details of models tested and methods of testing of the Eiffel 36 wing alone and the JN2 aircraft. Characteristics and performance curves for standard JN are included. Part 2 presents a statistical analysis of the following: lift and drag contributed by body and chassis tested without wings; lift and drag contributed by tail, tested without wings; the effect on lift and drift of interference between the wings of a biplane combination; lift and drag contributed by the addition of body, chassis, and tail to a biplane combination; total parasite resistance; effect of varying size of tail, keeping angle of setting constant; effect of varying length of body and size of tail at the same time, keeping constant moment of tail surface about the center of gravity; forces on the tail and the effects of downwash; effect of size and setting of tail on statical longitudinal stability effects of length of body on stability; the effects of the various elements of an airplane on longitudinal stability and the placing of the force vectors. Part 3 presents the fundamental principals of dynamical stability; computations of resistance derivatives; solution of the stability equation; dynamical stability of the Curtiss JN2; tabulation of resistance derivatives; discussion of the resistance derivatives; formation and solution of stability equations; physical conceptions of the resistance derivatives; elements contributing to damping and an investigation of low speed conditions. Part 4 includes a summary of the results of the statistical investigation and a summary of the results for dynamic stability.

Some Effects of Horizontal-Tail Position on the Vertical-Tail Pressure Distributions of a Complete Model in Sideslip at High Subsonic Speeds

NASA Technical Reports Server (NTRS)

Alford, William J., Jr.

1958-01-01

An investigation has been made in the Langley high-speed 7- by 10-foot tunnel of some effects of horizontal-tail position on the vertical-tail pressure distributions of a complete model in sideslip at high subsonic speeds. The wing of the model was swept back 28.82 deg at the quarter-chord line and had an aspect ratio of 3.50, a taper ratio of 0.067, and NACA 65A004 airfoil sections parallel to the model plane of symmetry. Tests were made with the horizontal tail off, on the wing-chord plane extended, and in T-tail arrangements in forward and rearward locations. The test Mach numbers ranged from 0.60 to 0.92, which corresponds to a Reynolds number range from approximately 2.93 x 10(exp 6) to 3.69 x 10(exp 6), based on the wing mean aerodynamic chord. The sideslip angles varied from -3.9 deg to 12.7 deg at several selected angles of attack. The results indicated that, for a given angle of sideslip, increases in angle of attack caused reductions in the vertical-tail loads in the vicinity of the root chord and increases at the midspan and tip locations, with rearward movements in the local chordwise centers of pressure for the midspan locations and forward movements near the tip of the vertical tail. At the higher angles of attack all configurations investigated experienced outboard and rearward shifts in the center of pressure of the total vertical-tail load. Location of the horizontal tail on the wing- chord plane extended produced only small effects on the vertical-tail loads and centers of pressure. Locating the horizontal tail at the tip of the vertical tail in the forward position caused increases in the vertical-tail loads; this configuration, however, experienced considerable reduction in loads with increasing Mach number. Location of the horizontal tail at the tip of the vertical tail in the rearward position produced the largest increases in vertical-tail loads per degree sideslip angle; this configuration experienced the smallest variations of loads with Mach number of any of the configurations investigated.

A study of static stability of airships

NASA Technical Reports Server (NTRS)

Rizzo, Frank

1924-01-01

The first section deals with the theoretical side of statical stability of airships in general. The second section deals with preliminary tests of the model and experiments for the determination of effects due to change of tail area, aspect ratio, tail form, and tail thickness.

A Nonparametric K-Sample Test for Equality of Slopes.

ERIC Educational Resources Information Center

Penfield, Douglas A.; Koffler, Stephen L.

1986-01-01

The development of a nonparametric K-sample test for equality of slopes using Puri's generalized L statistic is presented. The test is recommended when the assumptions underlying the parametric model are violated. This procedure replaces original data with either ranks (for data with heavy tails) or normal scores (for data with light tails).…

Flexible growing rods: a pilot study to determine if polymer rod constructs may provide stability to skeletally immature spines

PubMed Central

2015-01-01

Background Surgical treatments for early onset scoliosis (EOS), including growing rod constructs, involve many complications. Some are due to biomechanical factors. A construct that is more flexible than current instrumentation systems may reduce complications. The purpose of this preliminary study was to determine spine range of motion (ROM) after implantation of simulated growing rod constructs with a range of clinically relevant structural properties. The hypothesis was that ROM of spines instrumented with polyetheretherketone (PEEK) rods would be greater than metal rods and lower than noninstrumented controls. Further, adjacent segment motion was expected to be lower with polymer rods compared to conventional systems. Methods Biomechanical tests were conducted on 6 skeletally immature porcine thoracic spines (domestic swine, 35-40 kg). Spines were harvested after death from swine that had been utilized for other studies (IACUC approved) which had not involved the spine. Paired pedicle screws were used as anchors at proximal and distal levels. Specimens were tested under the following conditions: control, then dual rods of PEEK (6.25 mm), titanium (4 mm), and CoCr (5 mm) alloy. Lateral bending (LB) and flexion-extension (FE) moments of ±5 Nm were applied. Vertebral rotations were measured using video. Differences were determined by two-tailed t-tests and Bonferroni correction with four primary comparisons: PEEK vs control and PEEK vs CoCr, in LB and FE (α=0.05/4). Results In LB, ROM of specimens with PEEK rods was lower than control at each instrumented level. ROM was greater for PEEK rods than both Ti and CoCr at every instrumented level. Mean ROM at proximal and distal noninstrumented levels was lower for PEEK than for Ti and CoCr. In FE, mean ROM at proximal and distal noninstrumented levels was lower for PEEK than for metal. Combining treated levels, in LB, ROM for PEEK rods was 35% of control (p<0.0001) and 270% of CoCr rods (p<0.01). In FE, ROM with PEEK was 27% of control (p<0.001) and 180% of CoCr (p<0.01). Conclusions PEEK rods decreased flexibility versus noninstumented controls, and increased flexibility versus metal rods. Smaller increases in ROM at proximal and distal adjacent motion segments occurred with PEEK compared to metal rods, which may help decrease junctional kyphosis. Flexible growing rods may eventually help improve treatment options for young patients with severe deformity. PMID:25810752

Mobility of as, Cu, Cr, and Zn from tailings covered with sealing materials using alkaline industrial residues: a comparison between two leaching methods.

PubMed

Jia, Yu; Maurice, Christian; Öhlander, Björn

2016-01-01

Different alkaline residue materials (fly ash, green liquor dregs, and lime mud) generated from the pulp and paper industry as sealing materials were evaluated to cover aged mine waste tailings (<1% sulfur content, primarily pyrite). The mobility of four selected trace elements (Cr, Cu, Zn, and As) was compared based on batch and column leaching studies to assess the effectiveness of these alkaline materials as sealing agents. Based on the leaching results, Cr, Cu, and Zn were immobilized by the alkaline amendments. In the amended tailings in the batch system only As dramatically exceeded the limit values at L/S 10 L/kg. The leaching results showed similar patterns to the batch results, though leached Cr, Cu, and Zn showed higher levels in the column tests than in the batch tests. However, when the columns were compared with the batches, the trend for Cu was opposite for the unamended tailings. By contrast, both batch and column results showed that the amendment caused mobilization of As compared with the unamended tailings in the ash-amended tailings. The amount of As released was greatest in the ash column and decreased from the dregs to the lime columns. The leaching of As at high levels can be a potential problem whenever alkaline materials (especially for fly ash) are used as sealing materials over tailings. The column test was considered by the authors to be a more informative method in remediation of the aged tailings with low sulfur content, since it mimics better actual situation in a field.

Near surface IP investigations: Four case studies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hearst, R.B.; Morris, W.A.; Clark, M.A.

1995-12-31

The use of the Induced Polarisation (IP) method of geophysical surveying for near surface site investigations is gaining acceptance within the geophysical community. In this study the IP method is evaluated as a tool for the delineation of ground water resources; contamination plume detection in a lateritic horizon; and acid mine drainage leak detection from decommissioned mine tailings. A time domain IP system was selected for this study primarily for the flexibility in the selection and setting of receiver time windows and diagnostic characteristics attributed to submitting the data to Cole-Cole analysis. Analysis of the acquired data in conjunction withmore » available borehole and geological information illustrates the effectiveness and usefulness of the survey method for solving near surface problems. In all of the locations tested, it was found that with a properly designed IP survey it was possible to resolve the target and/or related structures.« less

Wind-Tunnel Investigation at Subsonic and Supersonic Speeds of a Fighter Model Employing a Low-Aspect-Ratio Unswept Wing and a Horizontal Tail Mounted Well Above the Wing Plane - Longitudinal Stability and Control

NASA Technical Reports Server (NTRS)

Smith, Williard G.

1954-01-01

Experimental results showing the static longitudinal-stability and control characteristics of a model of a fighter airplane employing a low-aspect-ratio unswept wing and an all-movable horizontal tail are presented. The investigation was made over a Mach number range from 0.60 to 0.90 and from 1.35 to 1.90 at a constant Reynolds number of 2.40 million, based on the wing mean aerodynamic chord. Because of the location of the horizontal tail at the tip of the vertical tail, interference was noted between the vertical tail and the horizontal tail and between the wing and the horizontal tail. This interference produced a positive pitching-moment coefficient at zero lift throughout the Mach number range of the tests, reduced the change in stability with increasing lift coefficient of the wing at moderate lift coefficients in the subsonic speed range, and reduced the stability at low lift coefficients at high supersonic speeds. The lift and pitching-moment effectiveness of the all movable tail was unaffected by the interference effects and was constant throughout the lift-coefficient range of the tests at each Mach number except 1.90.

Effect of empennage location on twin-engine afterbody-nozzle aerodynamic characteristics at Mach Numbers from 0.6 to 1.2. [wind tunnel tests

NASA Technical Reports Server (NTRS)

Leavitt, L. D.

1983-01-01

The Langley 16-foot transonic tunnel was used to determine the effects of several empennage and afterbody parameters on the aft-end aerodynamic characteristics of a twin-engine fighter-type configuration. Model variables were as follows: horizontal tail axial location and incidence, vertical tail axial location and configuration (twin- versus single-tail arrangements), tail booms, and nozzle power setting. Tests were conducted over a Mach number range from 0.6 to 1.2 and over an angle-of-attack from -2 deg to 10 deg. Jet total-pressure ratio was varied from jet off to approximately 10.0.

Subsonic Aerodynamic Characteristics of an Airplane Configuration with a 63 deg Sweptback Wing and Twin-Boom Tails

NASA Technical Reports Server (NTRS)

Savage, Howard F.; Edwards, George G.

1959-01-01

A wind-tunnel investigation has been conducted to determine the effects of an unconventional tail arrangement on the subsonic static longitudinal and lateral stability characteristics of a model having a 63 deg sweptback wing of aspect ratio 3.5 and a fuselage. Tail booms, extending rearward from approximately the midsemispan of each wing panel, supported independent tail assemblies well outboard of the usual position at the rear of the fuselage. The horizontal-tail surfaces had the leading edge swept back 45 deg and an aspect ratio of 2.4. The vertical tail surfaces were geometrically similar to one panel of the horizontal tail. For comparative purposes, the wing-body combination was also tested with conventional fuselage-mounted tail surfaces. The wind-tunnel tests were conducted at Mach numbers from 0.25 to 0.95 with a Reynolds number of 2,000,000, at a Mach number of 0.46 with a Reynolds number of 3,500,000, and at a Mach number of 0.20 with a Reynolds number of 7,000,000. The results of the investigation indicate that longitudinal stability existed to considerably higher lift coefficients for the outboard tail configuration than for the configuration with conventional tail. Wing fences were necessary with both configurations for the elimination of sudden changes in longitudinal stability at lift coefficients between 0.3 and 0.5. Sideslip angles up to 15 deg had only small effects upon the pitching-moment characteristics of the outboard tail configuration. There was an increase in the directional stability for the outboard tail configuration at the higher angles of attack as opposed to a decrease for the conventional tail configuration at most of the Mach numbers and Reynolds numbers of this investigation. The dihedral effect increased rapidly with increasing angle of attack for both the outboard and the conventional tail configurations but the increase was greater for the outboard tail configuration. The data indicate that the outboard tail is an effective roll control.

Damped flexible seal

DOEpatents

DuBois, Neil J.; Amaral, Antonio M.

1992-10-27

A damped flexible seal assembly for a torpedo isolates the tailcone thereof rom vibrational energy present in the drive shaft assembly. A pair of outside flanges, each of which include an inwardly facing groove and an O-ring constrained therein, provide a watertight seal against the outer non-rotating surface of the drive shaft assembly. An inside flange includes an outwardly-facing groove and an O-ring constrained therein, and provides a watertight seal against the inner surface of the tail cone. Two cast-in-place elastomeric seals provide a watertight seal between the flanges and further provide a damping barrier between the outside flanges and the inside flanges for damping vibrational energy present in the drive shaft assembly before the energy can reach the tailcone through the seal assembly.

SMART Rotor Development and Wind Tunnel Test

DTIC Science & Technology

2009-09-01

amplifier and control system , and data acquisition, processing, and display systems . Boeing�s LRTS (Fig. 2), consists of a sled structure that...Support Test Stand Sled Tail Sting Outrigger Arm Figure 2: System integration test at whirl tower Port Rotor Balance Main Strut Flap Tail...demonstrated. Finally, the reliability of the flap actuation system was successfully proven in more than 60 hours of wind tunnel testing

AN/ALQ-135 tail warning system follow-on operational test and evaluation

NASA Astrophysics Data System (ADS)

Jenkins, V. M.

1981-11-01

This is a study of the reliability and maintainability of the tail warning system (TWS) AN/ALQ-153. This TWS is a solid state pulsed Doppler radar designed to provide warning and initiate countermeasures against threats attacking from the tail of B-52G/H aircraft.

Experiments on a Tail-wheel Shimmy

NASA Technical Reports Server (NTRS)

Harling, R; Dietz, O

1954-01-01

Model tests on the "running belt" and tests with a full-scale tail wheel were made on a rotating drum as well as on a runway in order to investigate the causes of the undesirable shimmy phenomena frequently occurring on airplane tail wheels, and the means of avoiding them. The small model (scale 1:10) permitted simulation of the mass, moments of inertia, and fuselage stiffness of the airplane and determination of their influence on the shimmy, whereas by means of the larger model with pneumatic tires (scale 1:2) more accurate investigations were made on the tail wheel itself. The results of drum and road tests show good agreement with one another and with model values. Detailed investigations were made regarding the dependence of the shimmy tendency on trail, rolling speed, load, size of tires, ground friction,and inclination of the swivel axis; furthermore, regarding the influence of devices with restoring effect on the tail wheel, and the friction damping required for prevention of shimmy. Finally observations from slow-motion pictures are reported and conclusions drawn concerning the influence of tire deformation.

Vertical Tail Buffeting Alleviation Using Piezoelectric Actuators: Some Results of the Actively Controlled Response of Buffet-Affected Tails (ACROBAT) Program

NASA Technical Reports Server (NTRS)

Moses, Robert W.

1997-01-01

A 1/6-scale F-18 wind-tunnel model was tested in the Transonic Dynamics Tunnel at the NASA Langley Research Center as part of the Actively Controlled Response Of Buffet Affected Tails (ACROBAT) program to assess the use of active controls in reducing vertical tail buffeting. The starboard vertical tail was equipped with an active rudder and the port vertical tail was equipped with piezoelectric actuators. The tunnel conditions were atmospheric air at a dynamic pressure of 14 psf. By using single-input-single-output control laws at gains well below the physical limits of the actuators, the power spectral density of the root strains at the frequency of the first bending mode of the vertical tail was reduced by as much as 60 percent up to angles of attack of 37 degrees. Root mean square (RMS) values of root strain were reduced by as much as 19 percent. Buffeting alleviation results when using the rudder are presented for comparison. Stability margins indicate that a constant gain setting in the control law may be used throughout the range of angle of attack tested.

3D-printing a 'family' of biomimetic models to explain armored grasping in syngnathid fishes.

PubMed

Porter, Michael M; Ravikumar, Nakul

2017-11-06

Seahorses and pipehorses evolved at least two independent strategies for tail grasping, despite being armored with a heavy body plating. To help explain mechanical trade-offs associated with the different designs, we created a 'family' of 3D-printed models that mimic variations in the presence and size of their armored plates. We measured the performance of the biomimetic proxies across several mechanical metrics, representative of their protective and prehensile capacities. Our results show that the models mimicking the tails of seahorses are the best all-around performers, while those of the distal-most, prehensile region of pipehorses are more flexible, but less protected. The comparison also reveals that different adaptive strategies provide different task-specific performance advantages, which could be leveraged for the design of armored manipulators or other bio-inspired technologies.

An experimental study of the effect of tail configuration on the spinning characteristics of general aviation aircraft. M.S. Thesis; [static wind tunnel force measurements

NASA Technical Reports Server (NTRS)

Ballin, M. G.

1982-01-01

The feasibility of using static wind tunnel tests to obtain information about spin damping characteristics of an isolated general aviation aircraft tail was investigated. A representative tail section was oriented to the tunnel free streamline at angles simulating an equilibrium spin. A full range of normally encountered spin conditions was employed. Results of parametric studies performed to determine the effect of spin damping on several tail design parameters show satisfactory agreement with NASA rotary balance tests. Wing and body interference effects are present in the NASA studies at steep spin attitudes, but agreement improves with increasing pitch angle and spin rate, suggesting that rotational flow effects are minimal. Vertical position of the horizontal stabilizer is found to be a primary parameter affecting yaw damping, and horizontal tail chordwise position induces a substantial effect on pitching moment.

Vertical Tail Buffeting Alleviation Using Piezoelectric Actuators-Some Results of the Actively Controlled Response of Buffet-Affected Tails (ACROBAT) Program

NASA Technical Reports Server (NTRS)

Moses, Robert W.

1997-01-01

Buffet is an aeroelastic phenomenon associated with high performance aircraft especially those with twin vertical tails. In particular, for the F/A-18 aircraft at high angles of attack, vortices emanating from wing/fuselage leading edge extensions burst, immersing the vertical tails in their wake. The resulting buffet loads on the vertical tails are a concern from fatigue and inspection points of view. Recently, a 1/6-scale F-18 wind-tunnel model was tested in the Transonic Dynamics Tunnel at the NASA Langley Research Center as part of the Actively Controlled Response Of Buffet Affected Tails (ACROBAT) Program to assess the use of active controls in reducing vertical tail buffeting. The starboard vertical tail was equipped with an active rudder and the port vertical tail was equipped with piezoelectric actuators. The tunnel conditions were atmospheric air at Mach 0.10. By using single-input-single-output control laws at gains well below the physical limits of the actuators, the power spectral density of the root strains at the frequency of the first bending mode of the vertical tail was reduced by as much as 60 percent up to angles of attack of 37 degrees. Root mean square (RMS) values of root strain were reduced by as much as 19 percent. The results herein illustrate that buffet alleviation of vertical tails can be accomplished using simple active control of the rudder or piezoelectric actuators. In fact, as demonstrated herein, a fixed gain single input single output control law that commands piezoelectric actuators may be active throughout the high angle-of-attack maneuver without requiring any changes during the maneuver. Future tests are mentioned for accentuating the international interest in this area of research.

An Investigation at Low Speed of the Spin Instability of Mortar-Shell Tails

NASA Technical Reports Server (NTRS)

Bird, John D.; Lichtenstein, Jacob H.

1957-01-01

An investigation was made in the Langley stability tunnel to study the influence of number of fins, fin shrouding, and fin aspect ratio on the spin instability of mortar-shell tail surfaces. It was found that the 12-fin tails tested spun less rapidly throughout the angle-of-yaw range than did the 6-fin tails and that fin shrouding reduced the spin encountered by a large amount.

Supersonic aerodynamic characteristics of conformal carriage monoplanar circular missile configurations with low-profile quadriform tail fins

NASA Technical Reports Server (NTRS)

Blair, A. B., Jr.

1990-01-01

Wind tunnel tests were conducted on monoplanar circular missile configurations with low-profile quadriform tail fins to provide an aerodynamic data base to study and evaluate air-launched missile candidates for efficient conformal carriage on supersonic-cruise-type aircraft. The tests were conducted at Mach numbers from 1.70 to 2.86 for a constant Reynolds number per foot of 2,000,000. Selected test results are presented to show the effects of tail-fin dihedral angle, wing longitudinal and vertical location, and nose-body strakes on the static longitudinal and lateral-directional aerodynamic stability and control characteristics.

Effect of nose shape and tail length on supersonic stability characteristics of a projectile

NASA Technical Reports Server (NTRS)

Sawyer, W. C.; Collins, I. K.

1973-01-01

The effect of nose shape and tail length on the static stability of a fin-stabilized projectile has been investigated in the Langley Unitary Plan with tunnel at angles of attack to about 12 deg for a Mach number range from 1.5 to 2.5. The tests were made at a constant Reynolds number of 6.56 x 1,000,000 per meter. The results of the investigation showed that nose shape had no effect on the static stability. Increasing the tail length resulted in a progressively stabilizing tendency. However, only the 1.5-caliber-tail-length configuration was stable over the test angle-of-attack range at Mach number 1.5. This configuration was marginally stable or unstable at the higher Mach numbers, and the shorter configurations were unstable at all Mach numbers for either part of or the entire test angle-of-attack range.

Performance Enhancement of a Full-Scale Vertical Tail Model Equipped with Active Flow Control

NASA Technical Reports Server (NTRS)

Whalen, Edward A.; Lacy, Douglas; Lin, John C.; Andino, Marlyn Y.; Washburn, Anthony E.; Graff, Emilio; Wygnanski, Israel J.

2015-01-01

This paper describes wind tunnel test results from a joint NASA/Boeing research effort to advance active flow control (AFC) technology to enhance aerodynamic efficiency. A full-scale Boeing 757 vertical tail model equipped with sweeping jet actuators was tested at the National Full-Scale Aerodynamics Complex (NFAC) 40- by 80-Foot Wind Tunnel (40x80) at NASA Ames Research Center. The model was tested at a nominal airspeed of 100 knots and across rudder deflections and sideslip angles that covered the vertical tail flight envelope. A successful demonstration of AFC-enhanced vertical tail technology was achieved. A 31- actuator configuration significantly increased side force (by greater than 20%) at a maximum rudder deflection of 30deg. The successful demonstration of this application has cleared the way for a flight demonstration on the Boeing 757 ecoDemonstrator in 2015.

Tests Of Avrocar Annular Jet VTOL Airplane in Ames 40x80 foot Wind Tunnel.

NASA Image and Video Library

1997-12-30

Rear view of the Avrocar without the tail, with ground board and variable height struts. The air force wanted to test the design of a flying saucer with vertical takeoff and landing capability. The design proved unstable without the tail.

Hypothesis Testing Using Spatially Dependent Heavy Tailed Multisensor Data

DTIC Science & Technology

2014-12-01

Office of Research 113 Bowne Hall Syracuse, NY 13244 -1200 ABSTRACT HYPOTHESIS TESTING USING SPATIALLY DEPENDENT HEAVY-TAILED MULTISENSOR DATA Report...consistent with the null hypothesis of linearity and can be used to estimate the distribution of a test statistic that can discrimi- nate between the null... Test for nonlinearity. Histogram is generated using the surrogate data. The statistic of the original time series is represented by the solid line

Statistical Tests Black swans or dragon-kings? A simple test for deviations from the power law★

NASA Astrophysics Data System (ADS)

Janczura, J.; Weron, R.

2012-05-01

We develop a simple test for deviations from power law tails. Actually, from the tails of any distribution. We use this test - which is based on the asymptotic properties of the empirical distribution function - to answer the question whether great natural disasters, financial crashes or electricity price spikes should be classified as dragon-kings or `only' as black swans.

The Effects of Tail Docking Method on Piglets' Behavioral Responses to a Formalin Pain Test

USDA-ARS?s Scientific Manuscript database

Routine piglet production procedures, for example teeth clipping, tail docking and castration, most likely cause pain and are under increasing scrutiny from the animal rights lobby. The objectives of this study were to assess the impact of 2 alternative methods of tail-docking on subsequent response...

Research on simulation technology of full-path infrared tail flame tracking of photoelectric theodolite in complicated environment

NASA Astrophysics Data System (ADS)

Wu, Hai-ying; Zhang, San-xi; Liu, Biao; Yue, Peng; Weng, Ying-hui

2018-02-01

The photoelectric theodolite is an important scheme to realize the tracking, detection, quantitative measurement and performance evaluation of weapon systems in ordnance test range. With the improvement of stability requirements for target tracking in complex environment, infrared scene simulation with high sense of reality and complex interference has become an indispensable technical way to evaluate the track performance of photoelectric theodolite. And the tail flame is the most important infrared radiation source of the weapon system. The dynamic tail flame with high reality is a key element for the photoelectric theodolite infrared scene simulation and imaging tracking test. In this paper, an infrared simulation method for the full-path tracking of tail flame by photoelectric theodolite is proposed aiming at the faint boundary, irregular, multi-regulated points. In this work, real tail images are employed. Simultaneously, infrared texture conversion technology is used to generate DDS texture for a particle system map. Thus, dynamic real-time tail flame simulation results with high fidelity from the theodolite perspective can be gained in the tracking process.

Active Vertical Tail Buffeting Alleviation on an F/A-18 Model in a Wind Tunnel

NASA Technical Reports Server (NTRS)

Moses, Robert W.

1999-01-01

A 1/6-scale F-18 wind-tunnel model was tested in the Transonic Dynamics Tunnel at the NASA Langley Research Center as part of the Actively Controlled Response Of Buffet-Affected Tails (ACROBAT) program to assess the use of active controls in reducing vertical tail buffeting. The starboard vertical tail was equipped with an active rudder and other aerodynamic devices, and the port vertical tail was equipped with piezoelectric actuators. The tunnel conditions were atmospheric air at a dynamic pressure of 14 psf. By using single-input-single-output control laws at gains well below the physical limits of the control effectors, the power spectral density of the root strains at the frequency of the first bending mode of the vertical tail was reduced by as much as 60 percent up to angles of attack of 37 degrees. Root mean square (RMS) values of root strain were reduced by as much as 19 percent. Stability margins indicate that a constant gain setting in the control law may be used throughout the range of angle of attack tested.

STUDIES IN RESEARCH METHODOLOGY. IV. A SAMPLING STUDY OF THE CENTRAL LIMIT THEOREM AND THE ROBUSTNESS OF ONE-SAMPLE PARAMETRIC TESTS,

DTIC Science & Technology

iconoclastic . Even at N=1024 these departures were quite appreciable at the testing tails, being greatest for chi-square and least for Z, and becoming worse in all cases at increasingly extreme tail areas. (Author)

Supersonic aerodynamic characteristics of a low-aspect-ratio missile model with wing and tail controls and with tails in line and interdigitated

NASA Technical Reports Server (NTRS)

Graves, E. B.

1972-01-01

A study has been made to determine the aerodynamic characteristics of a low-aspect ratio cruciform missile model with all-movable wings and tails. The configuration was tested at Mach numbers from 1.50 to 4.63 with the wings in the vertical and horizontal planes and with the wings in a 45 deg roll plane with tails in line and interdigitated.

Effects of Topical Anesthetics on Behavior, Plasma Corticosterone, and Blood Glucose Levels after Tail Biopsy of C57BL/6NHSD Mice (Mus musculus)

PubMed Central

Dudley, Emily S; Johnson, Robert A; French, DeAnne C; Boivin, Gregory P

2016-01-01

Tail biopsy is a common procedure that is performed to obtain genetic material for determining genotype of transgenic mice. The use of anesthetics or analgesics is recommended, although identifying safe and effective drugs for this purpose has been challenging. We evaluated the effects of topical 2.5% lidocaine–2.5% prilocaine cream applied to the distal tail tip at 5 or 60 min before biopsy, immersion of the tail tip for 10 seconds in ice-cold 70% ethanol just prior to biopsy, and immersion of the tail tip in 0.5% bupivacaine for 30 s after biopsy. Mice were 7, 11, or 15 d old at the time of tail biopsy. Acute behavioral responses, plasma corticosterone, and blood glucose were measured after biopsy, and body weight and performance in elevated plus maze and open-field tests after weaning. Ice-cold ethanol prior to biopsy prevented acute behavioral responses to biopsy, and both ice-cold ethanol and bupivacaine prevented elevations in corticosterone and blood glucose after biopsy. Tail biopsy with or without anesthesia did not affect body weight or performance on elevated plus maze or open-field tests. We recommend the use of ice-cold ethanol for topical anesthesia prior to tail biopsy in mice 7 to 15 d old. PMID:27423152

Effects of Topical Anesthetics on Behavior, Plasma Corticosterone, and Blood Glucose Levels after Tail Biopsy of C57BL/6NHSD Mice (Mus musculus).

PubMed

Dudley, Emily S; Johnson, Robert A; French, DeAnne C; Boivin, Gregory P

2016-01-01

Tail biopsy is a common procedure that is performed to obtain genetic material for determining genotype of transgenic mice. The use of anesthetics or analgesics is recommended, although identifying safe and effective drugs for this purpose has been challenging. We evaluated the effects of topical 2.5% lidocaine-2.5% prilocaine cream applied to the distal tail tip at 5 or 60 min before biopsy, immersion of the tail tip for 10 seconds in ice-cold 70% ethanol just prior to biopsy, and immersion of the tail tip in 0.5% bupivacaine for 30 s after biopsy. Mice were 7, 11, or 15 d old at the time of tail biopsy. Acute behavioral responses, plasma corticosterone, and blood glucose were measured after biopsy, and body weight and performance in elevated plus maze and open-field tests after weaning. Ice-cold ethanol prior to biopsy prevented acute behavioral responses to biopsy, and both ice-cold ethanol and bupivacaine prevented elevations in corticosterone and blood glucose after biopsy. Tail biopsy with or without anesthesia did not affect body weight or performance on elevated plus maze or open-field tests. We recommend the use of ice-cold ethanol for topical anesthesia prior to tail biopsy in mice 7 to 15 d old.

Structural Insights into the Role of the Cyclic Backbone in a Squash Trypsin Inhibitor*

PubMed Central

Daly, Norelle L.; Thorstholm, Louise; Greenwood, Kathryn P.; King, Gordon J.; Rosengren, K. Johan; Heras, Begoña; Martin, Jennifer L.; Craik, David J.

2013-01-01

MCoTI-II is a head-to-tail cyclic peptide with potent trypsin inhibitory activity and, on the basis of its exceptional proteolytic stability, is a valuable template for the design of novel drug leads. Insights into inhibitor dynamics and interactions with biological targets are critical for drug design studies, particularly for protease targets. Here, we show that the cyclization and active site loops of MCoTI-II are flexible in solution, but when bound to trypsin, the active site loop converges to a single well defined conformation. This finding of reduced flexibility on binding is in contrast to a recent study on the homologous peptide MCoTI-I, which suggested that regions of the peptide are more flexible upon binding to trypsin. We provide a possible explanation for this discrepancy based on degradation of the complex over time. Our study also unexpectedly shows that the cyclization loop, not present in acyclic homologues, facilitates potent trypsin inhibitory activity by engaging in direct binding interactions with trypsin. PMID:24169696

MagnetoSperm: A microrobot that navigates using weak magnetic fields

NASA Astrophysics Data System (ADS)

Khalil, Islam S. M.; Dijkslag, Herman C.; Abelmann, Leon; Misra, Sarthak

2014-06-01

In this work, a propulsion system similar in motion to a sperm-cell is investigated. This system consists of a structure resembling a sperm-cell with a magnetic head and a flexible tail of 42 μm and 280 μm in length, respectively. The thickness, length, and width of this structure are 5.2 μm, 322 μm, and 42 μm, respectively. The magnetic head includes a 200 nm-thick cobalt-nickel layer. The cobalt-nickel layer provides a dipole moment and allows the flexible structure to align along oscillating weak (less than 5 mT) magnetic field lines, and hence generates a propulsion thrust force that overcomes the drag force. The frequency response of this system shows that the propulsion mechanism allows for swimming at an average speed of 158 ± 32 μm/s at alternating weak magnetic field of 45 Hz. In addition, we experimentally demonstrate controlled steering of the flexible structure towards reference positions.

Charge transfer and charge localization in extended radical cations: Investigation of model molecules for peptides

NASA Astrophysics Data System (ADS)

Weinkauf, Rainer; Lehrer, Florian

1998-12-01

Molecules consisting of a flexible tail and an aromatic chromophore are used as model systems to understand the situation of a single chromophore in a small peptide. Their S0-S1 resonant multiphoton ionization (REMPI) spectra show, that in neutral molecules the tail-chromophore interaction is weak and electronic excitation is localized at the chromophore. For molecules, where the ionization energy of the tail is considerable higher than that of the chromophore, by high resolution REMPI photoelectron spectroscopy we find the charge to be localized on the aromatic chromophore. This scheme also in suitable peptides allows local ionization at the aromatic chromophore. An estimate for various charge positions in peptide chains, however, shows, that for most of the amino acids electron hole positions in the nitrogen and oxygen "lone pair" orbitals of the peptide bond are nearly degenerate. REMPI photoelectron spectra of phenylethylamine, which as a model system contains such two degenerate charge positions, show small energetic shift of the ionization energy but strong geometry changes upon electron removal. This result is interpreted as direct ionization into a mixed charge delocalized state. Consequences for the charge transfer mechanism in peptides are discussed.

Untangleing the effects of chain rigidity on the structure and dynamics of strongly adsorbed polymer melts

DOE PAGES

Carrillo, Jan-Michael Y.; Cheng, Shiwang; Kumar, Rajeev; ...

2015-06-11

Here, we present a detailed analysis of coarse-grained molecular dynamics simulations of semiflexible polymer melts in contact with a strongly adsorbing substrate. We have characterized the segments in the interfacial layer by counting the number of trains, loops, tails and unadsorbed segments. For more rigid chains, a tail and an adsorbed segment (a train) dominate while loops are more prevalent in more flexible chains. The tails exhibit a non-uniformly stretched conformation akin to the polydispersed pseudobrush envisioned by Guiselin. To probe the dynamics of the segments we computed the layer z-resolved intermediate coherent collective dynamics structure factor, S(q, t, z),more » mean-square displacement of segments, and the 2nd Legendre polynomial of the time-autocorrelation of unit bond vectors, 2[n i(t,z)•n i(0,z)]>. Our results show that segmental dynamics is slower for stiffer chains and there is a strong correlation between the structure and dynamics in the interfacial layer. There is no glassy layer, and the slowing down in dynamics of stiffer chains in the adsorbed region can be attributed to the densification and the more persistent layering of segments.« less

Spiral swimming of an artificial micro-swimmer

NASA Astrophysics Data System (ADS)

Keaveny, Eric E.; Maxey, Martin R.

A device constructed from a filament of paramagnetic beads connected to a human red blood cell will swim when subject to an oscillating magnetic field. Bending waves propagate from the tip of the tail toward the red blood cell in a fashion analogous to flagellum beating, making the artificial swimmer a candidate for studying what has been referred to as micro-swimming. In this study, we demonstrate that under the influence of a rotating field the artificial swimmer will perform -type swimming. We conduct numerical simulations of the swimmer where the paramagnetic tail is represented as a series of rigid spheres connected by flexible but inextensible links. An optimal range of parameters governing the relative strength of viscous, elastic and magnetic forces is identified for swimming speed. A parameterization of the motion is extracted and examined as a function of the driving frequency. With a continuous elastica/resistive force model, we obtain an expression for the swimming speed in the low-frequency limit. Using this expression we explore further the effects of the applied field, the ratio of the transverse field to the constant field, and the ratio of the radius of the sphere to the length of the filament tail on the resulting dynamics.

Heavy-tailed distributions in the intermittent motion behaviour of the intertidal gastropod Littorina littorea

NASA Astrophysics Data System (ADS)

Seuront, Laurent; Duponchel, Anne-Charlotte; Chapperon, Coraline

2007-11-01

The two-dimensional motion behaviour of the common intertidal gastropod Littorina littorea is investigated as a function of the immersion time from three sampling sites on an exposed rocky shore. A total of 90 individuals have been individually marked and tracked over 14 consecutive daylight low tide. Successive displacements show very intermittent behaviour, with a few localised large displacements over a wide range of small displacements. We show that successive displacements are described by flight length l d heavy-tailed distributions with P(ld)∼ld-μ. The very low values of the exponent μ ( μ≈2.22, 2.43 and 2.67) indicate that L. littorea flights fall into the category of super-diffusive processes. These exponents were significantly higher than the special value μ≈2 analytically and theoretically predicted to be the most advantageous in optimising long-term encounter statistics, especially for low-prey-density scenario. As natural selection should favour flexible behaviour, leading to different optimum searching statistics, under different conditions, our results support the idea that the differences in food concentration and distribution encountered at the different sites by L. littorea led to different heavy-tailed distributions observed for the most extreme displacements.

Nonlinear dynamics of C-terminal tails in cellular microtubules

NASA Astrophysics Data System (ADS)

Sekulic, Dalibor L.; Sataric, Bogdan M.; Zdravkovic, Slobodan; Bugay, Aleksandr N.; Sataric, Miljko V.

2016-07-01

The mechanical and electrical properties, and information processing capabilities of microtubules are the permanent subject of interest for carrying out experiments in vitro and in silico, as well as for theoretical attempts to elucidate the underlying processes. In this paper, we developed a new model of the mechano-electrical waves elicited in the rows of very flexible C-terminal tails which decorate the outer surface of each microtubule. The fact that C-terminal tails play very diverse roles in many cellular functions, such as recruitment of motor proteins and microtubule-associated proteins, motivated us to consider their collective dynamics as the source of localized waves aimed for communication between microtubule and associated proteins. Our approach is based on the ferroelectric liquid crystal model and it leads to the effective asymmetric double-well potential which brings about the conditions for the appearance of kink-waves conducted by intrinsic electric fields embedded in microtubules. These kinks can serve as the signals for control and regulation of intracellular traffic along microtubules performed by processive motions of motor proteins, primarly from kinesin and dynein families. On the other hand, they can be precursors for initiation of dynamical instability of microtubules by recruiting the proper proteins responsible for the depolymerization process.

Optimizing an undulating magnetic microswimmer for cargo towing

NASA Astrophysics Data System (ADS)

Or, Yizhar; Gutman, Emiliya

2015-11-01

One of the promising applications of robotic microswimmers is towing a cargo for controlled drug delivery, micro-surgery or tumor detection. This capability has been demonstrated by the magnetically-actuated microswimmer of Dreyfus et al. [Nature 2005] in which a red blood cell was attached to a chain of magnetic beads connected by flexible DNA links. A key question is what should be the optimal size of the magnetic tail for towing a given cargo. This question is addressed here for the simplest theoretical model of a magnetic microswimmer under planar undulations - a spherical load connected by a torsion spring to a magnetized rigid slender link. The swimmer's dynamics is formulated assuming negligible hydrodynamic interaction and leading-order expressions for the resulting motion are obtained explicitly under small amplitude approximation. Optimal combinations of magnetic actuation frequency, torsion stiffness, and tail length for maximizing displacement or average speed are obtained. The theoretical results are compared with several reported magnetic microswimmers, and also agree qualitatively with recent results on cargo towing by screw rotation of magnetic helical tails [Walker et al., ACS Nano Letters 2015]. This work is supported by the Israeli Science Foundation (ISF) under Grant No. 567/14.

Design and Development of the Space Shuttle Tail Service Masts

NASA Technical Reports Server (NTRS)

Dandage, S. R.; Herman, N. A.; Godfrey, S. E.; Uda, R. T.

1977-01-01

The successful launch of a space shuttle vehicle depends on the proper operation of two tail service masts (TSMs). Reliable TSM operation is assured through a comprehensive design, development, and testing program. The results of the concept verification test (CVT) and the resulting impact on prototype TSM design are presented. The design criteria are outlined, and the proposed prototype TSM tests are described.

A Numerical Study of Vortex Dynamics of Flexible Wing Propulsors

DTIC Science & Technology

2009-11-23

of validation. Figure 2 shows the streamline plot of the cylinder for Reynolds number of 40. The coefficient of drag and blob length is calculated to...Large amplitude lunate tail theory of fish locomotion. Journal of Fluid Mechanics 74, 161–182. Clough, R. W. & Penzein, J. 1993 Dynamics of...the swimming of slender fish . Journal of Fluid Mechanics 9, 305–317. Lighthill, M. J. 1970 Aquatic animal propulsion of high hydrodynamical efficiency

Calculation of the aerodynamic loading of swept and unswept flexible wings of arbitrary stiffness

NASA Technical Reports Server (NTRS)

Diederich, Franklin W

1950-01-01

A method is presented for calculating the aerodynamic loading, the divergence speed, and certain stability derivatives of swept and unswept wings and tail surfaces of arbitrary stiffness. Provision is made for using either stiffness curves and root rotation constants or structural influence coefficients in the analysis. Computing forms, tables of numerical constants required in the analysis, and an illustrative example are included to facilitate calculations by means of the method.

Thermotropic liquid crystals from biomacromolecules

PubMed Central

Liu, Kai; Chen, Dong; Marcozzi, Alessio; Zheng, Lifei; Su, Juanjuan; Pesce, Diego; Zajaczkowski, Wojciech; Kolbe, Anke; Pisula, Wojciech; Müllen, Klaus; Clark, Noel A.; Herrmann, Andreas

2014-01-01

Complexation of biomacromolecules (e.g., nucleic acids, proteins, or viruses) with surfactants containing flexible alkyl tails, followed by dehydration, is shown to be a simple generic method for the production of thermotropic liquid crystals. The anhydrous smectic phases that result exhibit biomacromolecular sublayers intercalated between aliphatic hydrocarbon sublayers at or near room temperature. Both this and low transition temperatures to other phases enable the study and application of thermotropic liquid crystal phase behavior without thermal degradation of the biomolecular components. PMID:25512508

Exploring the atomic structure and conformational flexibility of a 320 Å long engineered viral fiber using X-ray crystallography.

PubMed

Bhardwaj, Anshul; Casjens, Sherwood R; Cingolani, Gino

2014-02-01

Protein fibers are widespread in nature, but only a limited number of high-resolution structures have been determined experimentally. Unlike globular proteins, fibers are usually recalcitrant to form three-dimensional crystals, preventing single-crystal X-ray diffraction analysis. In the absence of three-dimensional crystals, X-ray fiber diffraction is a powerful tool to determine the internal symmetry of a fiber, but it rarely yields atomic resolution structural information on complex protein fibers. An 85-residue-long minimal coiled-coil repeat unit (MiCRU) was previously identified in the trimeric helical core of tail needle gp26, a fibrous protein emanating from the tail apparatus of the bacteriophage P22 virion. Here, evidence is provided that an MiCRU can be inserted in frame inside the gp26 helical core to generate a rationally extended fiber (gp26-2M) which, like gp26, retains a trimeric quaternary structure in solution. The 2.7 Å resolution crystal structure of this engineered fiber, which measures ∼320 Å in length and is only 20-35 Å wide, was determined. This structure, the longest for a trimeric protein fiber to be determined to such a high resolution, reveals the architecture of 22 consecutive trimerization heptads and provides a framework to decipher the structural determinants for protein fiber assembly, stability and flexibility.

ArrayVigil: a methodology for statistical comparison of gene signatures using segregated-one-tailed (SOT) Wilcoxon's signed-rank test.

PubMed

Khan, Haseeb Ahmad

2005-01-28

Due to versatile diagnostic and prognostic fidelity molecular signatures or fingerprints are anticipated as the most powerful tools for cancer management in the near future. Notwithstanding the experimental advancements in microarray technology, methods for analyzing either whole arrays or gene signatures have not been firmly established. Recently, an algorithm, ArraySolver has been reported by Khan for two-group comparison of microarray gene expression data using two-tailed Wilcoxon signed-rank test. Most of the molecular signatures are composed of two sets of genes (hybrid signatures) wherein up-regulation of one set and down-regulation of the other set collectively define the purpose of a gene signature. Since the direction of a selected gene's expression (positive or negative) with respect to a particular disease condition is known, application of one-tailed statistics could be a more relevant choice. A novel method, ArrayVigil, is described for comparing hybrid signatures using segregated-one-tailed (SOT) Wilcoxon signed-rank test and the results compared with integrated-two-tailed (ITT) procedures (SPSS and ArraySolver). ArrayVigil resulted in lower P values than those obtained from ITT statistics while comparing real data from four signatures.

Experimental trim drag values for conventional and supercritical wings. M.S. Thesis

NASA Technical Reports Server (NTRS)

Jacobs, P. F.

1981-01-01

Supercritical wings were studied to determine whether they incur higher trim drag values at cruise conditions than wide body technology wings. Relative trim drag increments were measured in an experimental wind tunnel investigation. The tests utilized high aspect ratio supercritical wing and a wide body wing in conjunction with five different horizontal tail configurations, mounted on a representative wide body fuselage. The three low tail configurations and two T tail configurations were chosen to measure the effects on horizontal tail size, location, and camber on the trim drag increments for the two wings. The increase in performance (lift to drag ratio) for supercritical wing over the wide body wing was 11 percent for both the optimum low tail and T tail configurations.

A Critique of One-Tailed Hypothesis Test Procedures in Business and Economics Statistics Textbooks.

ERIC Educational Resources Information Center

Liu, Tung; Stone, Courtenay C.

1999-01-01

Surveys introductory business and economics statistics textbooks and finds that they differ over the best way to explain one-tailed hypothesis tests: the simple null-hypothesis approach or the composite null-hypothesis approach. Argues that the composite null-hypothesis approach contains methodological shortcomings that make it more difficult for…

Single Molecule Stepping and Structural Dynamics of Myosin X

PubMed Central

Sun, Yujie; Sato, Osamu; Ruhnow, Felix; Arsenault, Mark E.; Ikebe, Mitsuo; Goldman, Yale E.

2010-01-01

Myosin X is an unconventional myosin with puzzling motility properties. We studied the motility of dimerized myosin X using single molecule fluorescence techniques – polTIRF, FIONA, and Parallax to measure rotation angles and 3-dimensional position of the molecule during its walk. It was found that Myosin X steps processively in a hand-over-hand manner following a left-handed helical path along both single actin filaments and bundles. Its step size and velocity are smaller on actin bundles than individual filaments, suggesting myosin X often steps onto neighboring filaments in a bundle. The data suggest that a previously postulated single α-helical domain mechanically extends the 3-IQ motif lever arm and either the neck-tail hinge or the tail is flexible. These structural features, in conjunction with the membrane and microtubule binding domains, enable myosin X to perform multiple functions on varied actin structures in cells. PMID:20364131

Data Storage and sharing for the long tail of science

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, B.; Pouchard, L.; Smith, P. M.

Research data infrastructure such as storage must now accommodate new requirements resulting from trends in research data management that require researchers to store their data for the long term and make it available to other researchers. We propose Data Depot, a system and service that provides capabilities for shared space within a group, shared applications, flexible access patterns and ease of transfer at Purdue University. We evaluate Depot as a solution for storing and sharing multiterabytes of data produced in the long tail of science with a use case in soundscape ecology studies from the Human- Environment Modeling and Analysismore » Laboratory. We observe that with the capabilities enabled by Data Depot, researchers can easily deploy fine-grained data access control, manage data transfer and sharing, as well as integrate their workflows into a High Performance Computing environment.« less

An analytical model and scaling of chordwise flexible flapping wings in forward flight.

PubMed

Kodali, Deepa; Kang, Chang-Kwon

2016-12-13

Aerodynamic performance of biological flight characterized by the fluid structure interaction of a flapping wing and the surrounding fluid is affected by the wing flexibility. One of the main challenges to predict aerodynamic forces is that the wing shape and motion are a priori unknown. In this study, we derive an analytical fluid-structure interaction model for a chordwise flexible flapping two-dimensional airfoil in forward flight. A plunge motion is imposed on the rigid leading-edge (LE) of teardrop shape and the flexible tail dynamically deforms. The resulting unsteady aeroelasticity is modeled with the Euler-Bernoulli-Theodorsen equation under a small deformation assumption. The two-way coupling is realized by considering the trailing-edge deformation relative to the LE as passive pitch, affecting the unsteady aerodynamics. The resulting wing deformation and the aerodynamic performance including lift and thrust agree well with high-fidelity numerical results. Under the dynamic balance, the aeroelastic stiffness decreases, whereas the aeroelastic stiffness increases with the reduced frequency. A novel aeroelastic frequency ratio is derived, which scales with the wing deformation, lift, and thrust. Finally, the dynamic similarity between flapping in water and air is established.

Parasites, diseases, and health status of sympatric populations of sika deer and white-tailed deer in Maryland and Virginia.

PubMed

Davidson, W R; Crow, C B

1983-10-01

In July 1981, investigations on parasites, diseases, and herd health status were conducted on sympatric populations of sika deer (Cervus nippon) and white-tailed deer (Odocoileus virginianus) from Blackwater National Wildlife Refuge (Maryland) and Chincoteague National Wildlife Refuge (Virginia) on the Delmarva Peninsula. Five adult deer of each species were collected from each location and subjected to thorough necropsy examinations and laboratory tests. White-tailed deer at both locations harbored protozoan, helminth, and arthropod parasites typically associated with this species throughout the southeastern United States. In contrast, sika deer at both locations harbored only light burdens of ticks, chiggers, and sarcocysts. Serologic tests for antibodies to seven infectious disease agents revealed evidence of exposure to bovine virus diarrhea (BVD) virus, infectious bovine rhinotracheitis virus, and parainfluenza3 virus in white-tailed deer, but only BVD virus in sika deer. At both locations the general health status of sika deer was superior to that of white-tailed deer.

Pulse-train Stimulation of Primary Somatosensory Cortex Blocks Pain Perception in Tail Clip Test

PubMed Central

Lee, Soohyun; Hwang, Eunjin; Lee, Dongmyeong

2017-01-01

Human studies of brain stimulation have demonstrated modulatory effects on the perception of pain. However, whether the primary somatosensory cortical activity is associated with antinociceptive responses remains unknown. Therefore, we examined the antinociceptive effects of neuronal activity evoked by optogenetic stimulation of primary somatosensory cortex. Optogenetic transgenic mice were subjected to continuous or pulse-train optogenetic stimulation of the primary somatosensory cortex at frequencies of 15, 30, and 40 Hz, during a tail clip test. Reaction time was measured using a digital high-speed video camera. Pulse-train optogenetic stimulation of primary somatosensory cortex showed a delayed pain response with respect to a tail clip, whereas no significant change in reaction time was observed with continuous stimulation. In response to the pulse-train stimulation, video monitoring and local field potential recording revealed associated paw movement and sensorimotor rhythms, respectively. Our results show that optogenetic stimulation of primary somatosensory cortex at beta and gamma frequencies blocks transmission of pain signals in tail clip test. PMID:28442945

Flight investigation of the effect of tail configuration on stall, spin, and recovery characteristics of a low-wing general aviation research airplane

NASA Technical Reports Server (NTRS)

Stough, H. Paul, III; Patton, James M., Jr.; Sliwa, Steven M.

1987-01-01

Flight tests were performed to investigate the stall, spin, and recovery characteristics of a low-wing, single-engine, light airplane with four interchangeable tail configurations. The four tail configurations were evaluated for effects of varying mass distribution, center-of-gravity position, and control inputs. The airplane tended to roll-off at the stall. Variations in tail configuration produced spins ranging from 40 deg to 60 deg angle of attack and turn rates of about 145 to 208 deg/sec. Some unrecoverable flat spins were encountered which required use of the airplane spin chute for recovery. For recoverable spins, antispin rudder followed by forward wheel with ailerons centered provided the quickest spin recovery. The moderate spin modes agreed very well with those predicted from spin-tunnel model tests, however, the flat spin was at a lower angle of attack and a slower rotation rate than indicated by the model tests.

A wind-tunnel investigation of parameters affecting helicopter directional control at low speeds in ground effect

NASA Technical Reports Server (NTRS)

Yeager, W. T., Jr.; Young, W. H., Jr.; Mantay, W. R.

1974-01-01

An investigation was conducted in the Langley full-scale tunnel to measure the performance of several helicopter tail-rotor/fin configurations with regard to directional control problems encountered at low speeds in ground effect. Tests were conducted at wind azimuths of 0 deg to 360 deg in increments of 30 deg and 60 deg and at wind speeds from 0 to 35 knots. The results indicate that at certain combinations of wind speed and wind azimuth, large increases in adverse fin force require correspondingly large increases in the tail-rotor thrust, collective pitch, and power required to maintain yaw trim. Changing the tail-rotor direction of rotation to top blade aft for either a pusher tail rotor (tail-rotor wake blowing away from fin) or a tractor tail rotor (tail-rotor wake blowing against fin) will alleviate this problem. For a pusher tail rotor at 180 deg wind azimuth, increases in the fin/tail-rotor gap were not found to have any significant influence on the overall vehicle directional control capability. Changing the tail rotor to a higher position was found to improve tail-rotor performance for a fin-off configuration at a wind azimuth of 180 deg. A V-tail configuration with a pusher tail rotor with top blade aft direction of rotation was found to be the best configuration with regard to overall directional control capability.

Quasi-steady state aerodynamics of the cheetah tail.

PubMed

Patel, Amir; Boje, Edward; Fisher, Callen; Louis, Leeann; Lane, Emily

2016-08-15

During high-speed pursuit of prey, the cheetah (Acinonyx jubatus) has been observed to swing its tail while manoeuvring (e.g. turning or braking) but the effect of these complex motions is not well understood. This study demonstrates the potential of the cheetah's long, furry tail to impart torques and forces on the body as a result of aerodynamic effects, in addition to the well-known inertial effects. The first-order aerodynamic forces on the tail are quantified through wind tunnel testing and it is observed that the fur nearly doubles the effective frontal area of the tail without much mass penalty. Simple dynamic models provide insight into manoeuvrability via simulation of pitch, roll and yaw tail motion primitives. The inertial and quasi-steady state aerodynamic effects of tail actuation are quantified and compared by calculating the angular impulse imparted onto the cheetah's body and its shown aerodynamic effects contribute to the tail's angular impulse, especially at the highest forward velocities. © 2016. Published by The Company of Biologists Ltd.

Quasi-steady state aerodynamics of the cheetah tail

PubMed Central

Boje, Edward; Fisher, Callen; Louis, Leeann; Lane, Emily

2016-01-01

ABSTRACT During high-speed pursuit of prey, the cheetah (Acinonyx jubatus) has been observed to swing its tail while manoeuvring (e.g. turning or braking) but the effect of these complex motions is not well understood. This study demonstrates the potential of the cheetah's long, furry tail to impart torques and forces on the body as a result of aerodynamic effects, in addition to the well-known inertial effects. The first-order aerodynamic forces on the tail are quantified through wind tunnel testing and it is observed that the fur nearly doubles the effective frontal area of the tail without much mass penalty. Simple dynamic models provide insight into manoeuvrability via simulation of pitch, roll and yaw tail motion primitives. The inertial and quasi-steady state aerodynamic effects of tail actuation are quantified and compared by calculating the angular impulse imparted onto the cheetah's body and its shown aerodynamic effects contribute to the tail's angular impulse, especially at the highest forward velocities. PMID:27412267

5-Methoxy-N,N-dimethyltryptamine-induced analgesia is blocked by alpha-adrenoceptor antagonists in rats.

PubMed Central

Archer, T.; Danysz, W.; Jonsson, G.; Minor, B. G.; Post, C.

1986-01-01

The effects of the alpha-adrenoceptor antagonists prazosin, phentolamine and yohimbine upon 5-methoxy-N,N-dimethyltryptamine (5-MeODMT)-induced analgesia were tested in the hot-plate, tail-flick and shock-titration tests of nociception with rats. Intrathecally injected yohimbine and phentolamine blocked or attenuated the analgesia produced by systemic administration of 5-MeODMT in all three nociceptive tests. Intrathecally administered prazosin attenuated the analgesic effects of 5-MeODMT in the hot-plate and tail-flick tests, but not in the shock titration test. Intrathecal yohimbine showed a dose-related lowering of pain thresholds in saline and 5-MeODMT-treated animals. Phentolamine and prazosin produced normal dose-related curves in the hot-plate test and biphasic effects in the shock titration and tail-flick tests. These results demonstrate a functional interaction between alpha 2-adrenoceptors and 5-HT agonist-induced analgesia at a spinal level in rats. PMID:2877697

Breeding short-tailed shearwaters buffer local environmental variability in south-eastern Australia by foraging in Antarctic waters.

PubMed

Berlincourt, Maud; Arnould, John P Y

2015-01-01

Establishing patterns of movements of free-ranging animals in marine ecosystems is crucial for a better understanding of their feeding ecology, life history traits and conservation. As central place foragers, the habitat use of nesting seabirds is heavily influenced by the resources available within their foraging range. We tested the prediction that during years with lower resource availability, short-tailed shearwaters (Puffinus tenuirostris) provisioning chicks should increase their foraging effort, by extending their foraging range and/or duration, both when foraging in neritic (short trips) and distant oceanic waters (long trips). Using both GPS and geolocation data-loggers, at-sea movements and habitat use were investigated over three breeding seasons (2012-14) at two colonies in southeastern Australia. Most individuals performed daily short foraging trips over the study period and inter-annual variations observed in foraging parameters where mainly due to few individuals from Griffith Island, performing 2-day trips in 2014. When performing long foraging trips, this study showed that individuals from both colonies exploited similar zones in the Southern Ocean. The results of this study suggest that individuals could increase their foraging range while exploiting distant feeding zones, which could indicate that short-tailed shearwaters forage in Antarctic waters not only to maintain their body condition but may also do so to buffer against local environmental stochasticity. Lower breeding performances were associated with longer foraging trips to distant oceanic waters in 2013 and 2014 indicating they could mediate reductions in food availability around the breeding colonies by extending their foraging range in the Southern Ocean. This study highlights the importance of foraging flexibility as a fundamental aspect of life history in coastal/pelagic marine central place foragers living in highly variable environments and how these foraging strategies are use to buffer this variability.

Varying Conditions for Hexanoic Acid Degradation with BioTiger™

DOE Office of Scientific and Technical Information (OSTI.GOV)

Foreman, Koji; Milliken, Charles; Brigmon, Robin

BioTiger™ (BT) is a consortium of 12 bacteria designed for petroleum waste biodegradation. BT is currently being studied and could be considered for bioremediation of the Athabasca oil sands refineries in Canada and elsewhere. The run-off ponds from the petroleum extraction processes, called tailings ponds, are a mixture of polycyclic aromatic hydrocarbons, naphthenic acids, hydrocarbons, toxic chemicals like heavy metals, water, and sand. Due to environmental regulations the oil industry would like to separate and degrade the hazardous chemical species from the tailings ponds while recycling the water. It has been shown that BT at 30 C° is able tomore » completely degrade 10 mM hexanoic acid (HA) co-metabolically with 0.2% yeast extract (w/v) in 48 hours when starting at 0.4 OD 600nm. After establishing this stable degradation capability, variations were tested to explore the wider parameters of BT activity in temperature, pH, intermediate degradation, co-metabolic dependence, and transfer stability. Due to the vast differences in temperature at various points in the refineries, a wide range of temperatures were assessed. The results indicate that BT retains the ability to degrade HA, a model surrogate for tailings pond contaminants, at temperatures ranging from 15°C to 35°C. Hexanamide (HAM) was shown to be an intermediate generated during the degradation of HA in an earlier work and HAM is completely degraded after 48 hours, indicating that HAM is not the final product of HA degradation. Various replacements for yeast extract were attempted. Glucose, a carbon source; casein amino acids, a protein source; additional ammonia, mimicking known media; and additional phosphate with Wolffe’s vitamins and minerals all showed no significant degradation of HA compared to control. Decreasing the yeast extract concentration (0.05%) demonstrated limited but significant degradation. Finally, serial inoculations of BT were performed to determine the stability of degradation over several generations. Overall, BT has shown to be moderately flexible for HA co-metabolic biodegradation.« less

Tail loss and thermoregulation in the common lizard Zootoca vivipara

NASA Astrophysics Data System (ADS)

Herczeg, Gábor; Kovács, Tibor; Tóth, Tamás; Török, János; Korsós, Zoltán; Merilä, Juha

2004-10-01

Tail autotomy in lizards is an adaptive strategy that has evolved to reduce the risk of predation. Since tail loss reduces body mass and moving ability—which in turn are expected to influence thermal balance—there is potential for a trade-off between tail autotomy and thermoregulation. To test this hypothesis, we studied a common lizard (Zootoca vivipara) population at high latitude, inhabiting a high-cost thermal environment. Z. vivipara is a small, non-territorial lizard known as a very accurate thermoregulator. We made two predictions: (1) the reduced body weight due to tail loss results in faster heating rate (a benefit), and (2) the reduction in locomotor ability after tail loss induces a shift to the use of thermally poorer microhabitats (a cost), thus decreasing the field body temperatures of active lizards. We did not find any effect of tail loss on heating rate in laboratory experiments conducted under different thermal conditions. Likewise, no significant relationship between tail condition and field body temperatures, or between tail condition and thermal microhabitat use, were detected. Thus, our results suggest that tail autotomy does not influence the accuracy of thermoregulation in small-bodied lizards.

Tail loss and thermoregulation in the common lizard Zootoca vivipara.

PubMed

Herczeg, Gábor; Kovács, Tibor; Tóth, Tamás; Török, János; Korsós, Zoltán; Merilä, Juha

2004-10-01

Tail autotomy in lizards is an adaptive strategy that has evolved to reduce the risk of predation. Since tail loss reduces body mass and moving ability-which in turn are expected to influence thermal balance-there is potential for a trade-off between tail autotomy and thermoregulation. To test this hypothesis, we studied a common lizard (Zootoca vivipara) population at high latitude, inhabiting a high-cost thermal environment. Z. vivipara is a small, non-territorial lizard known as a very accurate thermoregulator. We made two predictions: (1) the reduced body weight due to tail loss results in faster heating rate (a benefit), and (2) the reduction in locomotor ability after tail loss induces a shift to the use of thermally poorer microhabitats (a cost), thus decreasing the field body temperatures of active lizards. We did not find any effect of tail loss on heating rate in laboratory experiments conducted under different thermal conditions. Likewise, no significant relationship between tail condition and field body temperatures, or between tail condition and thermal microhabitat use, were detected. Thus, our results suggest that tail autotomy does not influence the accuracy of thermoregulation in small-bodied lizards.

Solidification of arsenic and heavy metal containing tailings using cement and blast furnace slag.

PubMed

Kim, Jung-Wook; Jung, Myung Chae

2011-01-01

The objective of this study is to examine the solidification of toxic elements in tailings by the use of cement and blast furnace slag. Tailings samples were taken at an Au-Ag mine in Korea. To examine the best mixing ratio of tailings and the mixture of ordinary Portland cement (OPC) and blast furnace slag (SG) of 5:5, 6:6, 7:3, and 8:2, the 7:3 ratio of tailings and OPC+SG was adapted. In addition, the mixing ratios of water and OPC + SG were applied to 10, 20, and 30 wt%. After 7, 14, and 28 days' curing, the UCS test was undertaken. A relatively high strength of solidified material (137.2 kg cm⁻² in average of 3 samples) at 28 days' curing was found in 20 wt% of water content (WC). This study also examined the leachability of arsenic and heavy metals (Cd, Cu, Pb, and Zn) under the Korean Standard Leaching Test, and it showed that the reductions in leachabilities of As and heavy metals of solidified samples were ranged from 76 to 99%. Thus, all the solidified samples were within the guidelines for special and hazardous waste materials by the Waste Management Act in Korea. In addition, the result of freeze-thaw cycle test of the materials indicated that the durability of the materials was sufficient. In conclusion, solidification using a 7:3 mixing ratio of tailings and a 1:1 mixture of OPC + SG with 20% of WC is one of the best methods for the remediation of arsenic and heavy metals in tailings and other contaminated materials.

Flight Tests of Various Tail Modifications on the Brewster XSBA-1 Airplane II : Measurements of Flying Qualities with Tail Configuration Number Two

NASA Technical Reports Server (NTRS)

Phillips, W.H.; Crane, H.L.

1943-01-01

Several tail modifications of the Brewster XSBA-1 scout-bomber were investigated and results compared. Modifications consisted of variation of the chord of the elevator and rudder while the total area of the surfaces is kept constant and variations of the total area of the vertical tail surface. Configuration number 2 reduced trim changes by 50 percent and reduced average elevator control force gradient from 30 to 27 pounds/g. Stick travel required to stall in maneuver was 4.6 inches.

A Structural Weight Estimation Program (SWEEP) for Aircraft. Volume 11 - Flexible Airloads Stand-Alone Program

DTIC Science & Technology

1974-06-01

stiffness, lb-in. I Integer used to designate wing strip number 2 I Airplanw pitching moment of inertia, slug ft 2 I Airplane yawing moment of inertia...slug ft J Integer used to designated wing-loading distribution, i.e., J-l, loading due to angle of attack J=2> loading due to flap deflection J-3...moment at intersection of load reference line and body interface station (for vertical tail), in.-lb Integer used to designate type of wing airload

Manx: Close air support aircraft preliminary design

NASA Technical Reports Server (NTRS)

Amy, Annie; Crone, David; Hendrickson, Heidi; Willis, Randy; Silva, Vince

1991-01-01

The Manx is a twin engine, twin tailed, single seat close air support design proposal for the 1991 Team Student Design Competition. It blends advanced technologies into a lightweight, high performance design with the following features: High sensitivity (rugged, easily maintained, with night/adverse weather capability); Highly maneuverable (negative static margin, forward swept wing, canard, and advanced avionics result in enhanced aircraft agility); and Highly versatile (design flexibility allows the Manx to contribute to a truly integrated ground team capable of rapid deployment from forward sites).

Field testing of fugitive dust control techniques at a uranium mill tailings pile - 1982 Field Test, Gas Hills, Wyoming.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Elmore, M.R.; Hartley, J.N.

A field test was conducted on a uranium tailings pile to evaluate the effectiveness of 15 chemical stabilizers for control of fugitive dust from uranium mill tailings. A tailings pile at the Federal American Partners (FAP) Uranium Mill, Gas Hills, Wyoming, was used for the field test. Preliminary laboratory tests using a wing tunnel were conducted to select the more promising stabilizers for field testing. Fourteen of the chemical stabilizers were applied with a field spray system pulled behind a tractor; one--Hydro Mulch--was applied with a hydroseeder. A portable weather station and data logger were installed to record the weathermore » conditions at the test site. After 1 year of monitoring (including three site visits), all of the stabilizers have degraded to some degree; but those applied at the manufacturers' recommended rate are still somewhat effective in reducing fugitive emissions. The following synthetic polymer emulsions appear to be the more effective stabilizers: Wallpol 40-133 from Reichold Chemicals, SP-400 from Johnson and March Corporation, and CPB-12 from Wen Don Corporation. Installed costs for the test plots ranged from $8400 to $11,300/ha; this range results from differences in stabilizer costs. Large-scale stabilization costs of the test materials are expected to range from $680 to $3600/ha based on FAP experience. Evaluation of the chemical stabilizers will continue for approximately 1 year. 2 references, 33 figures, 22 tables.« less

Long-range-corrected Rung 3.5 density functional approximations

NASA Astrophysics Data System (ADS)

Janesko, Benjamin G.; Proynov, Emil; Scalmani, Giovanni; Frisch, Michael J.

2018-03-01

Rung 3.5 functionals are a new class of approximations for density functional theory. They provide a flexible intermediate between exact (Hartree-Fock, HF) exchange and semilocal approximations for exchange. Existing Rung 3.5 functionals inherit semilocal functionals' limitations in atomic cores and density tails. Here we address those limitations using range-separated admixture of HF exchange. We present three new functionals. LRC-ωΠLDA combines long-range HF exchange with short-range Rung 3.5 ΠLDA exchange. SLC-ΠLDA combines short- and long-range HF exchange with middle-range ΠLDA exchange. LRC-ωΠLDA-AC incorporates a combination of HF, semilocal, and Rung 3.5 exchange in the short range, based on an adiabatic connection. We test these in a new Rung 3.5 implementation including up to analytic fourth derivatives. LRC-ωΠLDA and SLC-ΠLDA improve atomization energies and reaction barriers by a factor of 8 compared to the full-range ΠLDA. LRC-ωΠLDA-AC brings further improvement approaching the accuracy of standard long-range corrected schemes LC-ωPBE and SLC-PBE. The new functionals yield highest occupied orbital energies closer to experimental ionization potentials and describe correctly the weak charge-transfer complex of ethylene and dichlorine and the hole-spin distribution created by an Al defect in quartz. This study provides a framework for more flexible range-separated Rung 3.5 approximations.

75 FR 70101 - Airworthiness Directives; Sikorsky Aircraft Corporation (Sikorsky) Model S-70A and S-70C Helicopters

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-17

... test (UT) inspection of the tail gearbox output bevel gear (gear) for a crack. If you find a crack... gear cracking incidents, one of which resulted in the tail rotor separating from the helicopter. The actions specified by this AD are intended to detect a crack in the gear to prevent a tail rotor separating...

Effect of suspension systems on the physiological and psychological responses to sub-maximal biking on simulated smoothand bumpy tracks.

PubMed

Titlestad, John; Fairlie-Clarke, Tony; Whittaker, Arthur; Davie, Mark; Watt, Ian; Grant, Stanley

2006-02-01

The aim of this study was to compare the physiological and psychological responses of cyclists riding on a hard tail bicycle and on a full suspension bicycle. Twenty males participated in two series of tests. A test rig held the front axle of the bicycle steady while the rear wheel rotated against a heavy roller with bumps (or no bumps) on its surface. In the first series of tests, eight participants (age 19-27 years, body mass 65-82 kg) were tested on both the full suspension and hard tail bicycles with and without bumps fitted to the roller. The second series of test repeated the bump tests with a further six participants (age 22-31 years, body mass 74-94 kg) and also involved an investigation of familiarization effects with the final six participants (age 21-30 years, body mass 64-80 kg). Heart rate, oxygen consumption (VO(2)), rating of perceived exertion (RPE) and comfort were recorded during 10 min sub-maximal tests. Combined data for the bumps tests show that the full suspension bicycle was significantly different (P < 0.001) from the hard tail bicycle on all four measures. Oxygen consumption, heart rate and RPE were lower on average by 8.7 (s = 3.6) ml . kg(-1) . min(-1), 32.1 (s = 12.1) beats . min(-1) and 2.6 (s = 2.0) units, respectively. Comfort scores were higher (better) on average by 1.9 (s = 0.8) units. For the no bumps tests, the only statistically significant difference (P = 0.008) was in VO(2), which was lower for the hard tail bicycle by 2.2 (s = 1.7) ml . kg(-1) . min(-1). The results indicate that the full suspension bicycle provides a physiological and psychological advantage over the hard tail bicycle during simulated sub-maximal exercise on bumps.

Graphite tail powder and liquid biofertilizer as trace elements source for ground nut

NASA Astrophysics Data System (ADS)

Hindersah, Reginawanti; Setiawati, M. Rochimi; Fitriatin, B. Natalie; Suryatama, Pujawati; Asmiran, Priyanka; Panatarani, Camellia; Joni, I. Made

2018-02-01

Utilization of graphite tail waste from the mineral beneficiation processing is very important since it contain significant amount of essential minerals which are necessary for plant growth. These mineral are required in biochemical processes and mainly play an important role as cofactor in enzymatic reaction. The objective of this research is to investigate the performance of graphite tail on supporting plant growth and yield of ground nut (Arachishypogeae L.). A field experiment has been performed to test the performance of mixed graphite tail and reduced organic matter dose. The graphite tail size were reduced to various sieved size, -80 mesh, -100 mesh and -200 mesh. The experiment was setup in randomized block design with 4 treatments and 6 replications for each treatment, while the control plot is received without graphite tail. The results demonstrated that reduced organic matter along with -200 mesh tail has potentially decreased plant height at the end of vegetative growth stage, in contrast for to -80 mesh tail amendment increased individual fresh plant biomass. Statistically, there was no change of plant nodule, individual shoot fresh and dry weight, root nodule, number of pod following any mesh of graphite tail amendment. Reducing organic matter while adding graphite tail of 5% did not change bean weight in all plot. In contrast, reduced organic matter along with 80-mesh graphite tail amendment improved the nut yield per plot. This experiment suggests that graphite tail, mainly -80 mesh graphite tail can be possibly used in legume production.

Acceptance by black-tailed deer of foliage treated with herbicides.

Treesearch

Dan L. Campbell; James Evans; Gerald D. Lindsey; William E. Dusenberry

1981-01-01

To test their acceptance of foliage treated with herbicides, captive black-tailed deer were exposed to Douglas-fir seedlings and salal treated with standard formulations of 2,4,5-T, 2,4-D, atrazine, dalapon, fosamine, and glyphosate herbicides. Carriers were diesel oil and water. Tests were made from November 1977 through February 1978. Deer readily browsed 2,4,5-T...

Methylation-sensitive amplification polymorphism analysis of fat and muscle tissues in pigs.

PubMed

Ma, J D; Li, M Z; Zhou, S L; Zhou, C W; Li, X W

2012-09-26

DNA methylation may be involved in regulating the expression of protein-coding genes, resulting in different fat and muscle phenotypes. Using a methylation-sensitive amplified polymorphism approach, we obtained 7423 bands by selective amplification of genomic DNA from six different fat depots and two heterogeneous muscle types from Duroc/Landrace/Yorkshire cross-bred pigs. The degrees of DNA methylation, determined by the percentages of hemi- and fully methylated sites relative to the total number of CCGG sites, were similar in male and female pigs for each specific tissue [χ(2) test; P (two-tailed) > 0.05]. Gender bias was therefore ignored. There were significant differences in the degree of DNA methylation among the eight tissue types [χ(2) test; P(total) (two-tailed) = 0.009]. However, similar degrees of methylation were observed among the six fat depots [χ(2) test; P(fat) (two-tailed) = 0.24 > 0.05]and between the two muscle types [χ(2) test; P(muscle) (two-tailed) = 0.76 > 0.05]. We conclude that the degree of DNA methylation differs between porcine fat and muscle tissue, but that the methylation status of a particular tissue type is similar, despite being deposited at different body sites.

Cognitive flexibility in young children: General or task-specific capacity?

PubMed

Deák, Gedeon O; Wiseheart, Melody

2015-10-01

Cognitive flexibility is the ability to adapt to changing tasks or problems. To test whether cognitive flexibility is a coherent cognitive capacity in young children, we tested 3- to 5-year-olds' performance on two forms of task switching, rule-based (Three Dimension Changes Card Sorting, 3DCCS) and inductive (Flexible Induction of Meaning-Animates and Objects, FIM-Ob and FIM-An), as well as tests of response speed, verbal working memory, inhibition, and reasoning. Results suggest that cognitive flexibility is not a globally coherent trait; only the two inductive word-meaning (FIM) tests showed high inter-test coherence. Task- and knowledge-specific factors also determine children's flexibility in a given test. Response speed, vocabulary size, and causal reasoning skills further predicted individual and age differences in flexibility, although they did not have the same predictive relation with all three flexibility tests. Copyright © 2015 Elsevier Inc. All rights reserved.

Two-Dimensional Fourier Transform Analysis of Helicopter Flyover Noise

NASA Technical Reports Server (NTRS)

SantaMaria, Odilyn L.; Farassat, F.; Morris, Philip J.

1999-01-01

A method to separate main rotor and tail rotor noise from a helicopter in flight is explored. Being the sum of two periodic signals of disproportionate, or incommensurate frequencies, helicopter noise is neither periodic nor stationary. The single Fourier transform divides signal energy into frequency bins of equal size. Incommensurate frequencies are therefore not adequately represented by any one chosen data block size. A two-dimensional Fourier analysis method is used to separate main rotor and tail rotor noise. The two-dimensional spectral analysis method is first applied to simulated signals. This initial analysis gives an idea of the characteristics of the two-dimensional autocorrelations and spectra. Data from a helicopter flight test is analyzed in two dimensions. The test aircraft are a Boeing MD902 Explorer (no tail rotor) and a Sikorsky S-76 (4-bladed tail rotor). The results show that the main rotor and tail rotor signals can indeed be separated in the two-dimensional Fourier transform spectrum. The separation occurs along the diagonals associated with the frequencies of interest. These diagonals are individual spectra containing only information related to one particular frequency.

Chemical and ecotoxicity evaluation of tailings rehabilitated using Technosol

NASA Astrophysics Data System (ADS)

Arán, Diego; Santos, Erika S.; Abreu, Maria Manuela; Macías, Felipe

2017-04-01

The Fé mining area was the most important uranium deposit in Spain. In this deposit, the uranium mineralization contains sulfides. Consequently, tailings are a source of polymetallic contamination requiring their rehabilitation in order to decrease the dispersion of potentially hazardous elements (PHEs). The main objective of this work is to evaluate the efficiency of a Technosol application on the rehabilitation of these tailings at chemical and ecotoxicological level. In the field, a layer of 20 cm Technosol with andic and eutrophic characteristic was applied over the tailing (total area: 625 m2). After 20 months, composite samples of Technosol (TEC), recovered tailing (bottom of the Technosol, RT) and tailings without recuperation (T) were collected. These samples were characterized for pH, electric conductivity (EC), PHEs concentration in total fraction and available fraction extracted with rhizosphere-based method. Ecotoxicity bioassays were carried out with two species, Lollium perenne and Trifolium pratense following OECD Guidelines. Three bioassays were carried out: filter paper test and hydroponic test with leachates, and soil test. In leachates (extracted with DIN method) were determined pH, EC and same PHEs than in Technosol/Tailings. Visual aspects, germination, root and shoot elongation and dry biomass were evaluated. The substrate effect on growth of both species was evaluated in pot experiment (500 g Technosol/Tailings per pot, 70% of water-holding capacity) under greenhouse conditions after 69 days by dry shoot biomass. Materials from T had pH 4, EC: 1.2 mS/cm and high total concentrations of several PHEs (g/kg; Al: 46.2; As, Co and Pb: 0.02-0.03; Cu: 0.04; Fe: 63.2 Mn: 1.3; Ni and Zn: 0.1-0.2). However, PHEs concentrations in leachates and available fraction corresponded to <2.5% of total concentrations, except for Co, Mn and Ni where 7-18% of their total concentrations can be available to organisms. Leachates from RT showed a significant improvement of pH values ( 6.5) and PHEs concentrations (>75% of reduction). For both species, the germination percentage and dry biomass weight in filter paper test (Lollium: 62-70%, 38-41 mg; Trifolium: 73-80%, 24-31 mg) and soil test (Lollium: 43-51%, 1.1-2.3 mg; Trifolium: 66-70%, 18-23 mg) did not presented significant differences. In hydroponic test, both species presented significant differences in elongation of shoots and roots but not for dry biomass. Tailing leachates inhibited shoots and roots elongation of Trifolium, however none negative effect was obtained for Lollium. In general, plants growing in TEC leachates had the greatest development. By the three bioassays, no clear evidences were obtained on ecotoxicological effect of the studied materials. In pot experiment, a total inhibition of Trifolium germination and a significant diminution of Lollium growth in T (<0.08 g) was obtained. Both species growing in TEC and RT produced quite similar shoot biomass (g; Lollium: 1.0-1.9, Trifollium: 1.9-2.7). Differences between OCDE tests and pot experiment are related with time of contact/assay. Results from pot experiments evidenced the efficiency of Technosol in the tailing rehabilitation and its potential revegetation by pastures. The authors thank ENUSA for technical cooperation and providing the study area and soil samples.

An Overview of Active Flow Control Enhanced Vertical Tail Technology Development

NASA Technical Reports Server (NTRS)

Lin, John C.; Andino, Marlyn Y.; Alexander, Michael G.; Whalen, Edward A.; Spoor, Marc A.; Tran, John T.; Wygnanski, Israel J.

2016-01-01

This paper summarizes a joint NASA/Boeing research effort to advance Active Flow Control (AFC) technology to enhance aerodynamic efficiency of a vertical tail. Sweeping jet AFC technology was successfully tested on subscale and full-scale models as well as in flight. The subscale test was performed at Caltech on a 14% scale model. More than 50% side force enhancement was achieved by the sweeping jet actuation when the momentum coefficient was 1.7%. AFC caused significant increases in suction pressure on the actuator side and associated side force enhancement. Subsequently, a full-scale Boeing 757 vertical tail model equipped with sweeping jets was tested at the National Full-Scale Aerodynamics Complex 40- by 80-Foot Wind Tunnel at NASA Ames Research Center. There, flow separation control optimization was performed at near flight conditions. Greater than 20% increase in side force were achieved for the maximum rudder deflection of 30deg at the key sideslip angles (0deg and -7.5deg) with a 31-actuator AFC configuration. Based on these tests, the momentum coefficient is shown to be a necessary, but not sufficient parameter to use for design and scaling of sweeping jet AFC from subscale tests to full-scale applications. Leveraging the knowledge gained from the wind tunnel tests, the AFC-enhanced vertical tail technology was successfully flown on the Boeing 757 ecoDemonstrator in the spring of 2015.

Experimental study of main rotor/tail rotor/airframe interactions in hover. Volume 1: Text and figures

NASA Technical Reports Server (NTRS)

Balch, D. T.; Saccullo, A.; Sheehy, T. W.

1983-01-01

To assist in identifying and quantifying the relevant parameters associated with the complex topic of main rotor/fuselage/tail rotor interference, a model scale hover test was conducted in the Model Rotor Hover Facility. The test was conducted using the basic model test rig, fuselage skins to represent a UH-60A BLACK HAWK helicopter, 4 sets of rotor blades of varying geometry (i.e., twist, airfoils and solidity) and a model tail rotor that could be relocated to give changes in rotor clearance (axially, laterally, and vertically), can't angle and operating model (pusher or tractor). The description of the models and the tests, data analysis and summary (including plots) are included. The customary system of units gas used for principal measurements and calculations. Expressions in both SI units and customary units are used with the SI units stated first and the customary units afterwords, in parenthesis.

Investigation of Reynolds Number Effects on a Generic Fighter Configuration in the National Transonic Facility

NASA Technical Reports Server (NTRS)

Tomek, W. G.; Hall, R. M.; Wahls, R. A.; Luckring, J. M.; Owens, L. R.

2002-01-01

A wind tunnel test of a generic fighter configuration was tested in the National Transonic Facility through a cooperative agreement between NASA Langley Research Center and McDonnell Douglas. The primary purpose of the test was to assess Reynolds number scale effects on a thin-wing, fighter-type configuration up to full-scale flight conditions (that is, Reynolds numbers of the order of 60 million). The test included longitudinal and lateral/directional studies at subsonic and transonic conditions across a range of Reynolds numbers from that available in conventional wind tunnels to flight conditions. Results are presented for three Mach numbers (0.6, 0.8, and 0.9) and three configurations: (1) Fuselage/Wing; (2) Fuselage/Wing/Centerline Vertical Tail/Horizontal Tail; and (3) Fuselage/Wing/Trailing-Edge Extension/Twin Vertical Tails. Reynolds number effects on the longitudinal aerodynamic characteristics are presented herein.

[Effect of simulated microgravity on peripheral oxygen saturation in rats].

PubMed

Chen, Guangfei; Zhang, Yahui; Yuan, Ming; He, Shilin; Ying, Jun; Li, Chen

2018-02-01

To study the effect of microgravity on peripheral oxygen saturation (SpO 2 ) in rats, tail-suspended rats were applied to simulate microgravity environment. SpO 2 and arterial oxygen saturation (SaO 2 ) were measured by pulse oximeter and arterial blood gas analyzer (ABGA) respectively on the 14th day, 21st day and 28th day in tail-suspended group and control group. Paired t -test shows that SpO 2 was significantly lower than SaO 2 in tail-suspended group on the 14th day ( P < 0.05), the 21st day ( P < 0.05) and the 28th day ( P < 0.01). The ANOVA results shows that modeling time had significant effect on SpO 2 value but no effect on SaO 2 value in tail-suspended group. These results indicate that pulse oximeter may be not suitable for oxygen saturation test in microgravity environment.

Tail Buffeting

NASA Technical Reports Server (NTRS)

Abdrashitov, G.

1943-01-01

An approximate theory of buffeting is here presented, based on the assumption of harmonic disturbing forces. Two cases of buffeting are considered: namely, for a tail angle of attack greater and less than the stalling angle, respectively. On the basis of the tests conducted and the results of foreign investigators, a general analysis is given of the nature of the forced vibrations the possible load limits on the tail, and the methods of elimination of buffeting.

Testing tail-mounted transmitters with Myocastor coypus (nutria)

USGS Publications Warehouse

Merino, S.; Carter, J.; Thibodeaux, G.

2007-01-01

We developed a tail-mounted radio-transmitter for Myocastor coypus (nutria) that offers a practical and efficient alternative to collar or implant methods. The mean retention time was 96 d (range 57-147 d, n = 7), making this a practical method for short-term studies. The tail-mounts were less injurious to animals than collars and easier for field researchers to implement than either collars or surgically implanted transmitters.

Magnetic fish-robot based on multi-motion control of a flexible magnetic actuator.

PubMed

Kim, Sung Hoon; Shin, Kyoosik; Hashi, Shuichiro; Ishiyama, Kazushi

2012-09-01

This paper presents a biologically inspired fish-robot driven by a single flexible magnetic actuator with a rotating magnetic field in a three-axis Helmholtz coil. Generally, magnetic fish-robots are powered by alternating and gradient magnetic fields, which provide a single motion such as bending the fish-robot's fins. On the other hand, a flexible magnetic actuator driven by an external rotating magnetic field can create several gaits such as the bending vibration, the twisting vibration, and their combination. Most magnetic fish-like micro-robots do not have pectoral fins on the side and are simply propelled by the tail fin. The proposed robot can swim and perform a variety of maneuvers with the addition of pectoral fins and control of the magnetic torque direction. In this paper, we find that the robot's dynamic actuation correlates with the magnetic actuator and the rotating magnetic field. The proposed robot is also equipped with new features, such as a total of six degrees of freedom, a new control method that stabilizes posture, three-dimensional swimming, a new velocity control, and new turning abilities.

Nonlinear dynamics of C–terminal tails in cellular microtubules

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sekulic, Dalibor L., E-mail: dalsek@uns.ac.rs; Sataric, Bogdan M.; Sataric, Miljko V.

2016-07-15

The mechanical and electrical properties, and information processing capabilities of microtubules are the permanent subject of interest for carrying out experiments in vitro and in silico, as well as for theoretical attempts to elucidate the underlying processes. In this paper, we developed a new model of the mechano–electrical waves elicited in the rows of very flexible C–terminal tails which decorate the outer surface of each microtubule. The fact that C–terminal tails play very diverse roles in many cellular functions, such as recruitment of motor proteins and microtubule–associated proteins, motivated us to consider their collective dynamics as the source of localizedmore » waves aimed for communication between microtubule and associated proteins. Our approach is based on the ferroelectric liquid crystal model and it leads to the effective asymmetric double-well potential which brings about the conditions for the appearance of kink–waves conducted by intrinsic electric fields embedded in microtubules. These kinks can serve as the signals for control and regulation of intracellular traffic along microtubules performed by processive motions of motor proteins, primarly from kinesin and dynein families. On the other hand, they can be precursors for initiation of dynamical instability of microtubules by recruiting the proper proteins responsible for the depolymerization process.« less

Structure of a bacterial virus DNA-injection protein complex reveals a decameric assembly with a constricted molecular channel

DOE PAGES

Zhao, Haiyan; Speir, Jeffrey A.; Matsui, Tsutomu; ...

2016-02-16

The multi-layered cell envelope structure of Gram-negative bacteria represents significant physical and chemical barriers for short-tailed phages to inject phage DNA into the host cytoplasm. Here we show that a DNA-injection protein of bacteriophage Sf6, gp12, forms a 465-kDa, decameric assembly in vitro. The electron microscopic structure of the gp12 assembly shows a ~150-Å, mushroom-like architecture consisting of a crown domain and a tube-like domain, which embraces a 25-Å-wide channel that could precisely accommodate dsDNA. The constricted channel suggests that gp12 mediates rapid, uni-directional injection of phage DNA into host cells by providing a molecular conduit for DNA translocation. Themore » assembly exhibits a 10-fold symmetry, which may be a common feature among DNA-injection proteins of P22-like phages and may suggest a symmetry mismatch with respect to the 6-fold symmetric phage tail. As a result, the gp12 monomer is highly flexible in solution, supporting a mechanism for translocation of the protein through the conduit of the phage tail toward the host cell envelope, where it assembles into a DNA-injection device.« less

Structure of a bacterial virus DNA-injection protein complex reveals a decameric assembly with a constricted molecular channel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhao, Haiyan; Speir, Jeffrey A.; Matsui, Tsutomu

The multi-layered cell envelope structure of Gram-negative bacteria represents significant physical and chemical barriers for short-tailed phages to inject phage DNA into the host cytoplasm. Here we show that a DNA-injection protein of bacteriophage Sf6, gp12, forms a 465-kDa, decameric assembly in vitro. The electron microscopic structure of the gp12 assembly shows a ~150-Å, mushroom-like architecture consisting of a crown domain and a tube-like domain, which embraces a 25-Å-wide channel that could precisely accommodate dsDNA. The constricted channel suggests that gp12 mediates rapid, uni-directional injection of phage DNA into host cells by providing a molecular conduit for DNA translocation. Themore » assembly exhibits a 10-fold symmetry, which may be a common feature among DNA-injection proteins of P22-like phages and may suggest a symmetry mismatch with respect to the 6-fold symmetric phage tail. As a result, the gp12 monomer is highly flexible in solution, supporting a mechanism for translocation of the protein through the conduit of the phage tail toward the host cell envelope, where it assembles into a DNA-injection device.« less

HARP model rotor test at the DNW. [Hughes Advanced Rotor Program

NASA Technical Reports Server (NTRS)

Dawson, Seth; Jordan, David; Smith, Charles; Ekins, James; Silverthorn, Lou

1989-01-01

Data from a test of a dynamically scaled model of the Hughes Advanced Rotor Program (HARP) bearingless model main rotor and 369K tail rotor are reported. The history of the HARP program and its goals are reviewed, and the main and tail rotor models are described. The test facilities and instrumentation are described, and wind tunnel test data are presented on hover, forward flight performance, and blade-vortex interaction. Performance data, acoustic data, and dynamic data from near field/far field and shear layer studies are presented.

Acoustic test of a model rotor and tail rotor: Results for the isolated rotors and combined configuration

NASA Technical Reports Server (NTRS)

Martin, R. M.; Burley, C. L.; Elliott, J. W.

1989-01-01

Acoustic data from a model scale main rotor and tail rotor experiment in the NASA Langley 14 by 22 Foot Subsonic Tunnel are presented for the main rotor and trail rotor in isolation and for the two rotors operating together. Results for the isolated main rotor show the importance of the rotor flapping conditions on mid-frequency noise content. High levels of main rotor retreating side blade-vortex interaction noise are shown to radiate downstream of the model. The isolated tail rotor noise results show the dominance of harmonic noise in the thrusting direction. The occurrence of tail rotor broadband noise is seen by the broadening of the tail rotor harmonics and is attributed to fuselage wake turbulence. The combined main and tail rotor data are presented to show the dominance of each rotor's different noise sources at different directivity locations.

Role of mycorrhizal colonization in plant establishment on an alkaline gold mine tailing.

PubMed

Orłowska, Elzbieta; Orłowski, Dariusz; Mesjasz-Przybyłowicz, Jolanta; Turnau, Katarzyna

2011-02-01

The potential role of arbuscular mycorrhizal fungi (AMF) in the revegetation of an alkaline gold mine tailing was studied in Barberton, South Africa. The tailing, characterized by a slow spontaneous plant succession, is colonized by the shrub Dodonaea viscosa and the grasses, Andropogon eucomus and Imperata cylindrica, all colonized by AMF. The effectiveness of mycorrhizal colonization in grasses was tested under laboratory conditions using fungal isolates of various origins. Both grasses were highly mycorrhiza dependent, and the presence of mycorrhizal colonization significantly increased their biomass and survival rates. The fungi originating from the gold tailing were better adapted to the special conditions of the tailing than the control isolate. Although the total colonization rate found for native fungi was lower than for fungi from non-polluted sites, they were more vital and more effective in promoting plant growth. The results obtained might serve as a practical approach to the phytostabilization of alkaline gold tailings.

In Vivo PET Imaging of Myelin Damage and Repair in the Spinal Cord

DTIC Science & Technology

2013-12-01

100, 110, 120 min(Pɘ.0001, two-tailed t- test , CI 99%). (B) the average radiance of wild-type mice after injection of DBT (blue) and vehicle ( red ...radiance between the Plp-Akt-DD mice ( red ) and wild-type mice (blue) after deducting the vehicle signals (P=0.0012, two-tailed t- test , CI 99...demyelination and remyelination in the intact brain and spinal cord. We have also begun to test the ability of the imaging probes to assay remyelination in

The Universal Statistical Distributions of the Affinity, Equilibrium Constants, Kinetics and Specificity in Biomolecular Recognition

PubMed Central

Zheng, Xiliang; Wang, Jin

2015-01-01

We uncovered the universal statistical laws for the biomolecular recognition/binding process. We quantified the statistical energy landscapes for binding, from which we can characterize the distributions of the binding free energy (affinity), the equilibrium constants, the kinetics and the specificity by exploring the different ligands binding with a particular receptor. The results of the analytical studies are confirmed by the microscopic flexible docking simulations. The distribution of binding affinity is Gaussian around the mean and becomes exponential near the tail. The equilibrium constants of the binding follow a log-normal distribution around the mean and a power law distribution in the tail. The intrinsic specificity for biomolecular recognition measures the degree of discrimination of native versus non-native binding and the optimization of which becomes the maximization of the ratio of the free energy gap between the native state and the average of non-native states versus the roughness measured by the variance of the free energy landscape around its mean. The intrinsic specificity obeys a Gaussian distribution near the mean and an exponential distribution near the tail. Furthermore, the kinetics of binding follows a log-normal distribution near the mean and a power law distribution at the tail. Our study provides new insights into the statistical nature of thermodynamics, kinetics and function from different ligands binding with a specific receptor or equivalently specific ligand binding with different receptors. The elucidation of distributions of the kinetics and free energy has guiding roles in studying biomolecular recognition and function through small-molecule evolution and chemical genetics. PMID:25885453

The Potential for Transmission of BCG from Orally Vaccinated White-Tailed Deer (Odocoileus virginianus) to Cattle (Bos taurus) through a Contaminated Environment: Experimental Findings

PubMed Central

Nol, Pauline; Rhyan, Jack C.; Robbe-Austerman, Suelee; McCollum, Matt P.; Rigg, Tara D.; Saklou, Nadia T.; Salman, Mo D.

2013-01-01

White-tailed deer (Odocoileus virginianus) experimentally infected with a virulent strain of Mycobacterium bovis have been shown to transmit the bacterium to other deer and cattle (Bos taurus) by sharing of pen waste and feed. The risk of transmission of M. bovis bacille Calmette-Guerin (BCG) vaccine from orally vaccinated white-tailed deer to other deer and cattle, however, is not well understood. In order to evaluate this risk, we orally vaccinated 14 white-tailed deer with 1×109 colony forming units BCG in lipid-formulated baits and housed them with nine non-vaccinated deer. Each day we exposed the same seven naïve cattle to pen space utilized by the deer to look for transmission between the two species. Before vaccination and every 60 days until the end of the study, we performed tuberculin skin testing on deer and cattle, as well as interferon-gamma testing in cattle, to detect cellular immune response to BCG exposure. At approximately 27 weeks all cattle and deer were euthanized and necropsied. None of the cattle converted on either caudal fold, comparative cervical tests, or interferon-gamma assay. None of the cattle were culture positive for BCG. Although there was immunological evidence that BCG transmission occurred from deer to deer, we were unable to detect immunological or microbiological evidence of transmission to cattle. This study suggests that the risk is likely to be low that BCG-vaccinated white-tailed deer would cause domestic cattle to react to the tuberculin skin test or interferon-gamma test through exposure to a BCG-contaminated environment. PMID:23565211

Laser acupuncture and analgesia: preliminary evidence for a transient and opioid-mediated effect

NASA Astrophysics Data System (ADS)

Whittaker, Peter

2006-02-01

Acupuncture is frequently used to treat pain. Although human pain quantification is difficult and often subjective, in rodent models the tail-flick test provides a well-established and objective assessment of analgesia. This test measures the time taken before a rat withdraws its tail from a heat source. Needle and electroacupuncture at the acupuncture point Spleen-6, located at the tibia's posterior margin above the medial malleolus, has been found to increase tail-flick time in rats. The aim of the current study was to determine if laser acupuncture had a similar effect. A 550 μm diameter optic fiber was used to irradiate Spleen-6 for 2 minutes (690 nm, 130 mW) in female Sprague-Dawley rats. In addition, control experiments were performed in which rats were subjected to sham treatment (restraint but no irradiation) or irradiation of an non-acupuncture point (the tail's dorsal surface, 1cm from the base) using the same laser parameters. The baseline tail-flick time was measured and 15 minutes later the laser acupuncture or the control protocols were performed and tail-flick time re-measured 10 minutes after treatment. Additional experiments were done in which the opioid-blocker naloxone (20 mg/kg, intraperitoneal injection) was administered one hour before laser acupuncture. Tailflick time increased after laser acupuncture (P = 0.0002), but returned to baseline values one hour later. In contrast, no increase was found after either sham treatment or tail irradiation. Pretreatment with naloxone attenuated the increase in tail-flick time. In summary, laser acupuncture exerts a transient analgesic effect which may act via an opioid-mediated mechanism.

A practice of ultra-fine tailings disposal as filling material in a gold mine.

PubMed

Deng, D Q; Liu, L; Yao, Z L; Song, K I-I L; Lao, D Z

2017-07-01

A practice of cemented backfill technology with ultra-fine tailings in a gold mine was comprehensively presented, and a series of tests were conducted in accordance with the peculiar properties of ultra-fine tailings and the mining technology conditions. The test results show that, the tailings from Shuiyindong Gold Mine have a great grinding fineness, with the average particle diameter 22.03 μm, in which the ultra-fine particles with the diameter below 20 μm occupying 66.13%. The analysis results of chemical components of tailings indicate that the content of SiO 2 is relatively low, i.e., 33.08%, but the total content of CaO, MgO and Al 2 O 3 is relatively high i.e., 36.5%. After the settlement of 4-6 h, the tailing slurry with the initial concentration of 40% has the maximum settling concentration of 54.692%, and the corresponding maximum settling unit weight is 1.497 g/cm 3 . During the field application, the ultra-fine tailings and PC32.5 cement were mixed with the cement-tailings ratios of 1:3-1:8, and the slurry concentration of 50 wt% was prepared. Using the slurry pump, the prepared cemented backfill slurries flowed into the goaf, and then the strength of the cemented backfill body met the mining technique requirements in Shuiyindong Gold Mine, where the ore body has a smooth occurrence, with the average thickness of approximately 2 m and the inclination angle ranging from 5 to 10°. Copyright © 2017 Elsevier Ltd. All rights reserved.

Differential analgesic effects of a mu-opioid peptide, [Dmt(1)]DALDA, and morphine.

PubMed

Shimoyama, Megumi; Szeto, Hazel H; Schiller, Peter W; Tagaito, Yugo; Tokairin, Hideyuki; Eun, Chong moon; Shimoyama, Naohito

2009-01-01

H-Dmt-D-Arg-Phe-Lys-NH(2) ([Dmt(1)]DALDA), a highly selective micro-opioid peptide, is potently analgesic after systemic and intrathecal administration but is less potent given intracerebroventricularly. This study was performed to further characterize the analgesic effects of [Dmt(1)]DALDA. We compared the effects of [Dmt(1)]DALDA and morphine after systemic administration in two different acute pain tests, the tail flick test and the paw withdrawal test, and examined how antagonizing the spinal opioid actions would affect their analgesic effects. [Dmt(1)]DALDA was markedly more potent in the tail flick test than in the hot plate test, while the potencies of morphine were similar in the two tests. Intrathecal naloxone completely blocked the effect of systemic [Dmt(1)]DALDA in the tail flick test, while it only partially blocked the effect of morphine. At higher doses that produced analgesia in the hot plate test, the effect of [Dmt(1)]DALDA in this test was only partially blocked by naloxone. Systemic [Dmt(1)]DALDA has a unique analgesic property clearly different from that of morphine and it has a propensity to produce spinal analgesia.

The Relationship of the Sit and Reach Test to Criterion Measures of Hamstring and Back Flexibility in Young Females.

ERIC Educational Resources Information Center

Jackson, Allen W.; Baker, Alice A.

1986-01-01

This study tested 100 female adolescents to determine the relationships of the sit and reach test, a component of the Health Related Fitness Test, with back and hamstring flexibility. Findings indicate the sit and reach test is moderately related to hamstring flexibility but not to back and low back flexibility. (Author/MT)

Acid production potentials of massive sulfide minerals and lead-zinc mine tailings: a medium-term study.

PubMed

Çelebi, Emin Ender; Öncel, Mehmet Salim; Kobya, Mehmet

2018-01-01

Weathering of sulfide minerals is a principal source of acid generation. To determine acid-forming potentials of sulfide-bearing materials, two basic approaches named static and kinetic tests are available. Static tests are short-term, and easily undertaken within a few days and in a laboratory. In contrast, kinetic tests are long-term procedures and mostly carried out on site. In this study, experiments were conducted over a medium-term period of 2 months, not as short as static tests and also not as long as kinetic tests. As a result, pH and electrical conductivity oscillations as a function of time, acid-forming potentials and elemental contents of synthetically prepared rainwater leachates of massive sulfides and sulfide-bearing lead-zinc tailings from abandoned and currently used deposition areas have been determined. Although the lowest final pH of 2.70 was obtained in massive pyrite leachate, massive chalcopyrite leachate showed the highest titrable acidity of 1.764 g H 2 SO 4 /L. On the other hand, a composite of currently deposited mine tailings showed no acidic characteristic with a final pH of 7.77. The composite abandoned mine tailing leachate had a final pH of 6.70, close to the final pH of massive galena and sphalerite leachates, and produced a slight titrable acidity of 0.130 g H 2 SO 4 /L.

Tank tests to determine the effect of varying design parameters of planing-tail hulls II : effect of varying depth of step, angle of after- body keel, length of afterbody chine, and gross load

NASA Technical Reports Server (NTRS)

Dawson, John R; Mckann, Robert; Hay, Elizabeth S

1946-01-01

The second part of a series of tests made in Langley tank no. 2 to determine the effect of varying design parameters of planing-tail hulls is presented. Results are given to show the effects on resistance characteristics of varying angle of afterbody keel, depth of step, and length of afterbody chine. The effect of varying the gross load is shown for one configuration. The resistance characteristics of planing-tail hulls are compared with those of a conventional flying-boat hull. The forces on the forebody and afterbody of one configuration are compared with the forces on a conventional hull. Increasing the angle of afterbody keel had small effect on hump resistance and no effect on high-speed resistance but increased free-to-trim resistance at intermediate speeds. Increasing the depth of step increased hump resistance, had little effect on high-speed resistance, and increased free-to-trim resistance at intermediate speeds. Omitting the chines on the forward 25 percent of the afterbody had no appreciable effect on resistance. Omitting 70 percent of the chine length had almost no effect on maximum resistance but broadened the hump and increased spray around the afterbody. Load-resistance ratio at the hump decreased more rapidly with increasing load coefficient for the planing-tail hull than for the representative conventional hull, although the load-resistance ratio at the hump was greater for the planing-tail hull than for the conventional hull throughout the range of loads tested. At speeds higher than hump speed, load-resistance ratio for the planing-tail hull was a maximum at a particular gross load and was slightly less at heavier and lighter gross loads. The planing-tail hull was found to have lower resistance than the conventional hull at both the hump and at high speeds, but at intermediate speeds there was little difference. The lower hump resistance of the planing-tail hull was attributed to the ability of the afterbody to carry a greater percentage of the total load while maintaining a higher value of load-resistance ratio.

Determination of acid forming potential of massive sulfide minerals and the tailings situated in lead/zinc mining district of Balya (NW Turkey)

NASA Astrophysics Data System (ADS)

Çelebi, E. Ender; Öncel, M. Salim

2016-12-01

Weathering of sulfide minerals is a major source of acid production in nature and especially in mining territories. Pyrite is not the only principal mineral that generates acid drainage: other sulfide minerals (sphalerite, galena, chalcopyrite, etc.) may also be responsible for acid production. In addition to massive sulfide minerals, sulfide-bearing mine tailings may also produce acid drainage due to oxidation and hydrolysis reactions in waste dumps. The lead/zinc (Pb/Zn) mining region in Balya and Balıkesir, in Turkey, has operated mines intensively since the 1860s; so that characterization of the sulfide minerals and tailings situated and formed around the mining site is of great importance to secure a sustainable environment. For this purpose, acid production and neutralization potentials of massive sulfide ores of the region, and in the Pb/Zn process facility mine tailings from ten different points of tailings dam, have been determined by applied conventional Acid-Base Accounting (ABA) and Net Acid Generation (NAG) static tests after chemical and mineralogical analysis. The NAG pH and net acid production potential (NAPP) values were compared on a chart in order to classify the samples as either acid generating or non-acid generating. According to the comparisons, the sulfide minerals were classified as potentially acid forming (PAF). Massive pyrite had the highest NAPP and NAG pH value of 1966.6 kg H2SO4/ton and 1.91, respectively and the galena had the lowest NAPP value of 558.9 kg H2SO4/ton. However, the sphalerite NAG leachate pH value of 4.30 was the highest in sulfide minerals so that the sphalerite plotted near the uncertainty reference border in the PAF zone. In the mine tailings, NAPP values of 105.9 kg H2SO4/ton on average and the NAG pH values of over 7.5 were determined. In addition to these tests, water leaching (agitation test) was carried out on tailings in order to generate more information. The tailings did not generate acidic leachates as they lie on limestone bed rock which neutralized the acidity.

A potential role for bat tail membranes in flight control.

PubMed

Gardiner, James D; Dimitriadis, Grigorios; Codd, Jonathan R; Nudds, Robert L

2011-03-30

Wind tunnel tests conducted on a model based on the long-eared bat Plecotus auritus indicated that the positioning of the tail membrane (uropatagium) can significantly influence flight control. Adjusting tail position by increasing the angle of the legs ventrally relative to the body has a two-fold effect; increasing leg-induced wing camber (i.e., locally increased camber of the inner wing surface) and increasing the angle of attack of the tail membrane. We also used our model to examine the effects of flying with and without a tail membrane. For the bat model with a tail membrane increasing leg angle increased the lift, drag and pitching moment (nose-down) produced. However, removing the tail membrane significantly reduced the change in pitching moment with increasing leg angle, but it had no significant effect on the level of lift produced. The drag on the model also significantly increased with the removal of the tail membrane. The tail membrane, therefore, is potentially important for controlling the level of pitching moment produced by bats and an aid to flight control, specifically improving agility and manoeuvrability. Although the tail of bats is different from that of birds, in that it is only divided from the wings by the legs, it nonetheless, may, in addition to its prey capturing function, fulfil a similar role in aiding flight control.

A Potential Role for Bat Tail Membranes in Flight Control

PubMed Central

Gardiner, James D.; Dimitriadis, Grigorios; Codd, Jonathan R.; Nudds, Robert L.

2011-01-01

Wind tunnel tests conducted on a model based on the long-eared bat Plecotus auritus indicated that the positioning of the tail membrane (uropatagium) can significantly influence flight control. Adjusting tail position by increasing the angle of the legs ventrally relative to the body has a two-fold effect; increasing leg-induced wing camber (i.e., locally increased camber of the inner wing surface) and increasing the angle of attack of the tail membrane. We also used our model to examine the effects of flying with and without a tail membrane. For the bat model with a tail membrane increasing leg angle increased the lift, drag and pitching moment (nose-down) produced. However, removing the tail membrane significantly reduced the change in pitching moment with increasing leg angle, but it had no significant effect on the level of lift produced. The drag on the model also significantly increased with the removal of the tail membrane. The tail membrane, therefore, is potentially important for controlling the level of pitching moment produced by bats and an aid to flight control, specifically improving agility and manoeuvrability. Although the tail of bats is different from that of birds, in that it is only divided from the wings by the legs, it nonetheless, may, in addition to its prey capturing function, fulfil a similar role in aiding flight control. PMID:21479137

Magnetically Driven Swimming of Nanoscale Colloidal Assemblies

NASA Astrophysics Data System (ADS)

Breidenich, Jennifer; Benkoski, Jason; Baird, Lance; Deacon, Ryan; Land, H. Bruce; Hayes, Allen; Keng, Pei; Pyun, Jeffrey

2009-03-01

At microscopic length scales, locomotion can only be generated through asymmetric conformation changes, such as the undulating flagellum employed by protozoa. This simple yet elegant design is optimized according to the dueling needs of miniaturization and the fluid dynamics of the low Reynolds number environment. In this study, we fabricate nanoscale colloidal assemblies that mimic the head + tail structure of flagellates. The assemblies consist of two types of magnetic colloids: 25 nm polystyrene-coated Co nanoparticles, and 250 nm polyethylene glycol coated magnetite nanoparticles. When mixed together in N-dimethylformamide, the Co nanoparticles assemble into flexible, segmented chains ranging in length from 1 - 5 μm. These chains then attach at one end to the larger magnetic beads due to magnetic attraction. This head + tail structure aligns with an external uniform magnetic field and is actuated by an oscillating transverse field. We examine the effects of Co nanoparticle concentration, magnetite bead concentration, magnetic field strength, and oscillation frequency on the formation of swimmers and the speed of locomotion.

Linking Neurons to Network Function and Behavior by Two-Photon Holographic Optogenetics and Volumetric Imaging.

PubMed

Dal Maschio, Marco; Donovan, Joseph C; Helmbrecht, Thomas O; Baier, Herwig

2017-05-17

We introduce a flexible method for high-resolution interrogation of circuit function, which combines simultaneous 3D two-photon stimulation of multiple targeted neurons, volumetric functional imaging, and quantitative behavioral tracking. This integrated approach was applied to dissect how an ensemble of premotor neurons in the larval zebrafish brain drives a basic motor program, the bending of the tail. We developed an iterative photostimulation strategy to identify minimal subsets of channelrhodopsin (ChR2)-expressing neurons that are sufficient to initiate tail movements. At the same time, the induced network activity was recorded by multiplane GCaMP6 imaging across the brain. From this dataset, we computationally identified activity patterns associated with distinct components of the elicited behavior and characterized the contributions of individual neurons. Using photoactivatable GFP (paGFP), we extended our protocol to visualize single functionally identified neurons and reconstruct their morphologies. Together, this toolkit enables linking behavior to circuit activity with unprecedented resolution. Copyright © 2017 Elsevier Inc. All rights reserved.

Experimental trim drag values and flow-field measurements for a wide-body transport model with conventional and supercritical wings

NASA Technical Reports Server (NTRS)

Jacobs, P. F.

1982-01-01

The purpose of this study was to determine if advanced supercritical wings incur higher trim drag values at cruise conditions than current wide body technology wings. Relative trim drag increments were measured in an experimental wind tunnel investigation conducted in the Langley 8 Foot Transonic Pressure Tunnel. The tests utilized a high aspect ratio supercritical wing and a wide body aircraft wing, in conjunction with five different horizontal tail configurations, mounted on a representative wide body fuselage. The three low tail and two T-tail configurations were designed to measure the effects of horizontal tail size, location, and camber on the trim drag increments for the two wings. Longitudinal force and moment data were taken at a Mach number of 0.82 and design cruise lift coefficients for the wide body and supercritical wings of 0.45 and 0.55, respectively. The data indicate that the supercritical wing does not have significantly higher trim drag than the wide body wing. A reduction in tail size, combined with relaxed static stability, produced trim drag reductions for both wings. The cambered tails had higher trim drag increments than the symmetrical tails for both wings, and the T-tail configurations had lower trim drag increments than the low tail configurations.

Field investigation on structural performance of the buried UPVC pipes with and without geogrid reinforcement

NASA Astrophysics Data System (ADS)

Teja, Akkineni Surya; Rajkumar, R.; Gokula Krishnan, B.; Aravindh, R.

2018-02-01

Buried pipes are used mainly for water supply and drainage besides many other applications such as oil, liquefied natural gas, coal slurries and mine tailings. The pipes used may be rigid (reinforced concrete, vitrified clay and ductile iron) or flexible (Steel, UPVC, aluminium, Fiber glass and High-density polyethylene) although the distinction between them is blurring. Flexible pipe design is governed by deflection or buckling. UPVC pipes are preferred due to light weight, long term chemical stability and cost efficiency. This project aims to study the load deformation behaviour of the buried pipe and stress variation across the cross section of the pipe under static loading along with the influence of depth of embedment, density of backfill on the deformation and stresses in pipe and the deformation behaviour of buried pipe when soil is reinforced with geogrid reinforcement and evaluate the structural performance of the pipe.

The utilization of forward osmosis for coal tailings dewatering

EPA Science Inventory

The feasibility of dewatering coal tailings slurry by forward osmosis (FO) membrane process was investigated in this research. A prototype cell was designed and used for the dewatering tests. A cellulosic FO membrane (Hydration Technology Innovations, LLC, Albany, OR) was used fo...

Mobilization and transport of metal-rich colloidal particles from mine tailings into soil under transient chemical and physical conditions.

PubMed

Lu, Cong; Wu, Yaoguo; Hu, Sihai; Raza, Muhammad Ali; Fu, Yilin

2016-04-01

Exposed mine tailing wastes with considerable heavy metals can release hazardous colloidal particles into soil under transient chemical and physical conditions. Two-layered packed columns with tailings above and soils below were established to investigate mobilization and transport of colloidal particles from metal-rich mine tailings into soil under transient infiltration ionic strength (IS: 100, 20, 2 mM) and flow rate (FR: 20.7, 41, and 62.3 mm h(-1)), with Cu and Pb as representatives of the heavy metals. Results show that the tailing particles within the colloidal size (below 2 μm) were released from the columns. A step-decrease in infiltration IS and FR enhanced, whereas a step-increase in the IS and FR restrained the release of tailing particles from the column. The effects of step-changing FR were unexpected due to the small size of the released tailing particles (220-342 nm, being not sensitive to hydrodynamic shear force), the diffusion-controlled particle release process and the relatively compact pore structure. The tailing particles present in the solution with tested IS were found negatively charged and more stable than soil particles, which provides favorable conditions for tailing particles to be transported over a long distance in the soil. The mobilization and transport of Cu and Pb from the tailings into soil were mediated by the tailing particles. Therefore, the inherent toxic tailing particles could be considerably introduced into soil under certain conditions (IS reduction or FR decrease), which may result in serious environmental pollution.

Robust functional regression model for marginal mean and subject-specific inferences.

PubMed

Cao, Chunzheng; Shi, Jian Qing; Lee, Youngjo

2017-01-01

We introduce flexible robust functional regression models, using various heavy-tailed processes, including a Student t-process. We propose efficient algorithms in estimating parameters for the marginal mean inferences and in predicting conditional means as well as interpolation and extrapolation for the subject-specific inferences. We develop bootstrap prediction intervals (PIs) for conditional mean curves. Numerical studies show that the proposed model provides a robust approach against data contamination or distribution misspecification, and the proposed PIs maintain the nominal confidence levels. A real data application is presented as an illustrative example.

Linking bovine tuberculosis on cattle farms to white-tailed deer and environmental variables using Bayesian hierarchical analysis

USGS Publications Warehouse

Walter, W. David; Smith, Rick; Vanderklok, Mike; VerCauterren, Kurt C.

2014-01-01

Bovine tuberculosis is a bacterial disease caused by Mycobacterium bovis in livestock and wildlife with hosts that include Eurasian badgers (Meles meles), brushtail possum (Trichosurus vulpecula), and white-tailed deer (Odocoileus virginianus). Risk-assessment efforts in Michigan have been initiated on farms to minimize interactions of cattle with wildlife hosts but research onM. bovis on cattle farms has not investigated the spatial context of disease epidemiology. To incorporate spatially explicit data, initial likelihood of infection probabilities for cattle farms tested for M. bovis, prevalence of M. bovis in white-tailed deer, deer density, and environmental variables for each farm were modeled in a Bayesian hierarchical framework. We used geo-referenced locations of 762 cattle farms that have been tested for M. bovis, white-tailed deer prevalence, and several environmental variables that may lead to long-term survival and viability of M. bovis on farms and surrounding habitats (i.e., soil type, habitat type). Bayesian hierarchical analyses identified deer prevalence and proportion of sandy soil within our sampling grid as the most supported model. Analysis of cattle farms tested for M. bovisidentified that for every 1% increase in sandy soil resulted in an increase in odds of infection by 4%. Our analysis revealed that the influence of prevalence of M. bovis in white-tailed deer was still a concern even after considerable efforts to prevent cattle interactions with white-tailed deer through on-farm mitigation and reduction in the deer population. Cattle farms test positive for M. bovis annually in our study area suggesting that the potential for an environmental source either on farms or in the surrounding landscape may contributing to new or re-infections with M. bovis. Our research provides an initial assessment of potential environmental factors that could be incorporated into additional modeling efforts as more knowledge of deer herd factors and cattle farm prevalence is documented.

Mycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of Buruli ulcer.

PubMed

Wallace, John R; Mangas, Kirstie M; Porter, Jessica L; Marcsisin, Renee; Pidot, Sacha J; Howden, Brian; Omansen, Till F; Zeng, Weiguang; Axford, Jason K; Johnson, Paul D R; Stinear, Timothy P

2017-04-01

Addressing the transmission enigma of the neglected disease Buruli ulcer (BU) is a World Health Organization priority. In Australia, we have observed an association between mosquitoes harboring the causative agent, Mycobacterium ulcerans, and BU. Here we tested a contaminated skin model of BU transmission by dipping the tails from healthy mice in cultures of the causative agent, Mycobacterium ulcerans. Tails were exposed to mosquito (Aedes notoscriptus and Aedes aegypti) blood feeding or punctured with sterile needles. Two of 12 of mice with M. ulcerans contaminated tails exposed to feeding A. notoscriptus mosquitoes developed BU. There were no mice exposed to A. aegypti that developed BU. Eighty-eight percent of mice (21/24) subjected to contaminated tail needle puncture developed BU. Mouse tails coated only in bacteria did not develop disease. A median incubation time of 12 weeks, consistent with data from human infections, was noted. We then specifically tested the M. ulcerans infectious dose-50 (ID50) in this contaminated skin surface infection model with needle puncture and observed an ID50 of 2.6 colony-forming units. We have uncovered a biologically plausible mechanical transmission mode of BU via natural or anthropogenic skin punctures.

Effects of afterbody boattail design and empennage arrangement on aeropropulsive characteristics of a twin-engine fighter model at transonic speeds

NASA Technical Reports Server (NTRS)

Bangert, Linda S.; Leavitt, Laurence D.; Reubush, David E.

1987-01-01

The effects of empennage arrangement and afterbody boattail design of nonaxisymmetric nozzles on the aeropropulsive characteristics of a twin-engine fighter-type model have been determined in an investigation conducted in the Langley 16-Foot Transonic Tunnel. Three nonaxisymmetric and one twin axisymmetric convergent-divergent nozzle configurations were tested with three different tail arrangements: a two-tail V-shaped arrangement; a staggered, conventional three-tail arrangement; and a four-tail arrangement similar to that on the F-18. Two of the nonaxisymmetric nozzles were also vectorable. Tests were conducted at Mach numbers from 0.60 to 1.20 over an angle-of-attack range from -3 deg to 9 deg. Nozzle pressure ratio was varied from 1 (jet off) to approximately 12, depending on Mach number. Results indicate that at design nozzle pressure ratio, the medium aspect ratio nozzle (with equal boattail angles on the nozzle sidewalls and upper and lower flaps) had the lowest zero angle of attack drag of the nonaxisymmetric nozzles for all tail configurations at subsonic Mach numbers. The drag levels of the twin axisymmetric nozzles were competitive with those of the medium-aspect-ratio nozzle at subsonic Mach number.

Effect of Tail Surfaces on the Base Drag of a Body of Revolution at Mach Numbers of 1.5 and 2.0

NASA Technical Reports Server (NTRS)

Spahr, J Richard; Dickey, Robert R

1951-01-01

Wind-tunnel tests were performed at Mach numbers of 1.5 and 2.0 to investigate the influence of tail surfaces on the base drag of a body of revolution without boattailing and having a turbulent boundary layer. The tail surfaces were of rectangular plan form of aspect ratio 2.33 and has symmetrical, circular-arc airfoil section. The results of the investigation showed that the addition of these tail surfaces with the trailing edges at or near the body base incurred a large increase in the base-drag coefficient. For a cruciform tail having a 10-percent-thick airfoil section, this increase was about 70 percent at a Mach number of 1.5 and 35 percent at a Mach number of 2.0. As the trailing edge of the tail was moved forward or rearward of the base by about one tail-chord length, the base-drag increment was reduced to nearly zero. The increments in base-drag coefficient due to the presence of 10-percent-thick tail surfaces were generally twice those for 5-percent-thick surfaces. The base-drag increments due to the presence of a cruciform tail were less than twice those for a plane tail. An estimate of the change in base pressure due to the tail surfaces was made, based on a simple superposition of the airfoil-pressure field onto the base-pressure field behind the body. A comparison of the results with the experimental values indicated that in most cases the trend in the variation of the base-drag increment with changes in tail position could be predicted by this approximate method but that the quantitative agreement at most tail locations was poor.

Mouse handling limits the impact of stress on metabolic endpoints.

PubMed

Ghosal, Sriparna; Nunley, Amanda; Mahbod, Parinaz; Lewis, Alfor G; Smith, Eric P; Tong, Jenny; D'Alessio, David A; Herman, James P

2015-10-15

Studies focused on end-points that are confounded by stress are best performed under minimally stressful conditions. The objective of this study was to demonstrate the impact of handling designed to reduce animal stress on measurements of glucose tolerance. A cohort of mice (CD1.C57BL/6) naïve to any specific handling was subjected to either a previously described "cup" handling method, or a "tail-picked" method in which the animals were picked up by the tail (as is common for metabolic studies). Following training, an elevated plus maze (EPM) test was performed followed by measurement of blood glucose and plasma corticosterone. A second cohort (CD1.C57BL/6) was rendered obese by exposure to a high fat diet, handled with either the tail-picked or cup method and subjected to an intraperitoneal glucose tolerance test. A third cohort of C57BL/6 mice was exposed to a cup regimen that included a component of massage and was subjected to tests of anxiety-like behavior, glucose homeostasis, and corticosterone secretion. We found that the cup mice showed reduced anxiety-like behaviors in the EPM coupled with a reduction in blood glucose levels compared to mice handled by the tail-picked method. Additionally, cup mice on the high fat diet exhibited improved glucose tolerance compared to tail-picked controls. Finally, we found that the cup/massage group showed lower glucose levels following an overnight fast, and decreased anxiety-like behaviors associated with lower stress-induced plasma corticosterone concentration compared to tail-picked controls. These data demonstrate that application of handling methods that reduce anxiety-like behaviors in mice mitigates the confounding contribution of stress to interpretation of metabolic endpoints (such as glucose tolerance). Copyright © 2015 Elsevier Inc. All rights reserved.

Mouse Handling Limits the Impact of Stress on Metabolic Endpoints

PubMed Central

Ghosal, Sriparna; Nunley, Amanda; Mahbod, Parinaz; Lewis, Alfor G.; Smith, Eric P.; Tong, Jenny; D’Alessio, David A.; Herman, James P.

2015-01-01

Studies focused on end-points that are confounded by stress are best performed under minimally stressful conditions. The objective of this study was to demonstrate the impact of handling designed to reduce animal stress on measurements of glucose tolerance. A cohort of mice (CD1.C57BL/6) naïve to any specific handling were subjected to either a previously described “cup” handling method, or a “tail-picked” method in which the animals were picked up by the tail (as is common for metabolic studies). Following training, an elevated plus maze (EPM) test was performed followed by measurement of blood glucose and plasma corticosterone. A second cohort (CD1.C57BL/6) was rendered obese by exposure to a high fat diet, handled with either the tail-picked or cup method and subjected to an intraperitoneal glucose tolerance test. A third cohort of C57BL/6 mice was exposed to a cup regimen that included a component of massage and was subjected to tests of anxiety-like behavior, glucose homeostasis, and corticosterone secretion. We found that the cup mice showed reduced anxiety-like behaviors in the EPM coupled with a reduction in blood glucose levels compared to mice handled by the tail-picked method. Additionally, cup mice on the high fat diet exhibited improved glucose tolerance compared to tail-picked controls. Finally, we found that the cup/massage group showed lower glucose levels following an overnight fast, and decreased anxiety-like behaviors associated with lower stress-induced plasma corticosterone concentration compared to tail-picked controls. These data demonstrate that application of handling methods that reduce anxiety-like behaviors in mice mitigates the confounding contribution of stress to interpretation of metabolic endpoints (such as glucose tolerance). PMID:26079207

Experimental aerodynamic characteristics for slender bodies with thin wings and tail at angles of attack from 0 deg to 58 deg and Mach numbers from 0.6 to 2.0

NASA Technical Reports Server (NTRS)

Jorgensen, L. H.; Nelson, E. R.

1976-01-01

An experimental investigation was conducted by wind tunnel to measure the static aerodynamic characteristics for bodies of circular and elliptic cross section with various thin flat plate wings and a thin tail consisting of horizontal and vertical parts. The wings had aspect ratios of 4 and taper ratios of about 0, 0.25, and 0.5. Two additional wings, which had taper ratios near 0.25 and aspect ratios of about 3 and 5, were also tested in combination with the bodies and tail. All wings had about the same planform area. The exposed area of the horizontal portion of the tail was about 33 to 36 percent of the exposed area of the wings. The exposed area of the vertical tail fin was about 22 to 24 percent of the exposed area of the wings. The elliptic body, with an a/b = 2 cross section, had the same length and axial distribution of cross sectional area as the circular body. The circular body had a cylindrical aftersection of fineness ratio 7, and it was tested with the wings and tail in combination with tangent ogive noses that had fineness ratios of 2.5, 3.0, 3.5, and 5.0. In addition, an ogive nose with a rounded tip and an ogive nose with two different nose strake arrangements were used. Nineteen configuration combinations were tested at Mach numbers of 0.6, 0.9, 1.5, and 2.0 at angles of attack from 0 to 58 deg. The Reynolds numbers, based on body base diameter, were about 4.3 X 100,000.

Active acoustic interference elicits echolocation changes in heterospecific bats.

PubMed

Jones, Te K; Wohlgemuth, Melville J; Conner, William E

2018-06-27

Echolocating bats often forage in the presence of both conspecific and heterospecific individuals who have the potential to produce acoustic interference. Recent studies have shown that at least one bat species, the Brazilian free-tailed bat ( Tadarida brasiliensis ), produces specialized social signals that disrupt the sonar of conspecific competitors. We herein discuss the differences between passive and active jamming signals and test whether heterospecific jamming occurs in species overlapping spatiotemporally as well as whether such interference elicits a jamming avoidance response (JAR). We compare the capture rates of tethered moths and the echolocation parameters of big brown bats ( Eptesicus fuscus ) challenged with the playback of the jamming signal normally produced by Brazilian free-tailed bats and playback of deconstructed versions of this signal. There were no differences in the capture rates of targets with and without the jamming signal although significant changes in both spectral and temporal features of the bats' echolocation were observed. These changes are consistent with improvements of the signal-to-noise ratio in the presence of acoustic interference. Accordingly, we propose to expand the traditional definition of the JAR, stating that echolocation changes in response to interference should decrease similarity between the two signals, to include any change that increases the ability to separate returning echoes from active jamming stimuli originating from conspecific and heterospecific organisms. Flexibility in echolocation is an important characteristic for overcoming various forms of acoustic interference and may serve a purpose in interspecific interactions as well as intraspecific ones. © 2018. Published by The Company of Biologists Ltd.

Vibration and aeroelastic analysis of highly flexible HALE aircraft

NASA Astrophysics Data System (ADS)

Chang, Chong-Seok

The highly flexible HALE (High Altitude Long Endurance) aircraft analysis methodology is of interest because early studies indicated that HALE aircraft might have different vibration and aeroelastic characteristics from those of conventional aircraft. Recently the computer code Nonlinear Aeroelastic Trim And Stability of HALE Aircraft (NATASHA) was developed under NASA sponsorship. NATASHA can predict the flight dynamics and aeroelastic behavior for HALE aircraft with a flying wing configuration. Further analysis improvements for NATASHA were required to extend its capability to the ground vibration test (GVT) environment and to both GVT and aeroelastic behavior of HALE aircraft with other configurations. First, the analysis methodology, based on geometrically exact fully intrinsic beam theory, was extended to treat other aircraft cofigurations. Conventional aircraft with flexible fuselage and tail can now be modeled by treating the aircraft as an assembly of beam elements. NATASHA is now applicable to any aircraft cofiguration that can be modeled this way. The intrinsic beam formulation, which is a fundamental structural modeling approach, is now capable of being applying to a structure consisting of multiple beams by relating the virtual displacements and rotations at points where two or more beam elements are connected to each other. Additional aspects are also considered in the analysis such as auxiliary elevator input in the horizontal tail and fuselage aerodynamics. Second, the modeling approach was extended to treat the GVT environment for HALE aircraft, which have highly flexible wings. GVT has its main purpose to provide modal characteristics for model validation. A bungee formulation was developed by the augmented Lagrangian method and coupled to the intrinsic beam formulation for the GVT modeling. After the coupling procedure, the whole formulation cannot be fully intrinsic because the geometric constraint by bungee cords makes the system statically indeterminate. Displacement and rotation variables need to be introduced, but only at points to which bungee cords are attached. Third, because many HALE aircraft are propeller driven, the structural modeling was extended to include an engine/nacelle/propeller system using a two-degree-of-freedom model with pitch and yaw angles. This step was undertaken to predict a dynamic instability called "whirl flutter," which can be exhibited in such HALE aircraft. It can investigate how the nacelle whirling and wing motions affect each other. For simplicity, two fundamental assumptions are made regarding the propeller aerodynamics and inertia matrix of two-bladed propeller system. The propeller airloads are evaluated by the constant approximation which uses the averaged values for one revolution per blade. Periodic side forces and hub moments are evaluated based on how they affect the trim condition determined by the constant approximation. The next assumption is for certain HALE aircraft which can use a two-bladed propeller system. The inertia matrix appears as periodic in time in the governing equations. If the periodic inertia effect is negligible, then the inertia matrix can be replaced by that of equivalent three-bladed propeller system so that the stability analysis can obviate the need for Floquet theory. These new development have been fully integrated into the current version of NATASHA. Finally, a parametric study for representative HALE aircraft is presented to show how the current methodology can be utilized as a unified preliminary analysis tool for the vibration and aeroelastic analysis of highly flexible HALE aircraft.

Reliability and factorial validity of flexibility tests for team sports.

PubMed

Sporis, Goran; Vucetic, Vlatko; Jovanovic, Mario; Jukic, Igor; Omrcen, Darija

2011-04-01

The main goal of this method paper was to evaluate the reliability and factorial validity of flexibility tests used in soccer, and to do crossvalidation study on 2 other team sports using handball and basketball players. The second aim was to compare the validity of the different tests and evaluate the flexibility of soccer players; the third was to determine the positional differences between attackers, defenders, and midfielders in all flexibility tests. One hundred and fifty (n = 150) elite male junior soccer players, members of the First Croatian Junior League Teams, and 60 (n = 60) handball and 60 (n = 60) basketball players also members of the First Croatian Junior League Teams volunteered to participate in the study, tested for the purpose of crossvalidation. The SAR and V-SAR had the greatest AVR and ICC. The within-subjects variation ranged from between 0.3 and 3.8%. The lowest value of CV was found between the LSPL and LSPR. Low to moderate statistically significant correlation coefficients were found among all the measured flexibility tests. It was observed that the greatest correlations existed between the SAR and V-SAR (r = 0.65) and between the LLSR and LLSL (r = 0.56). Statistically significant correlations were also observed between the BLPL and BLPR (r = 0.62). The principal components factor analysis of 9 flexibility tests resulted in the extraction of 3 significant components. The results of this study have the following implications for the assessment of flexibility in soccer: (a) all flexibility tests used in this study have the acceptable between and within-subjects reliability and they can be used to estimate the flexibility of soccer players; (b) the LSPL and LSPR tests are the most reliable and valid flexibility tests for the estimation of flexibility of professional soccer players.

The tail of the ParG DNA segregation protein remodels ParF polymers and enhances ATP hydrolysis via an arginine finger-like motif

PubMed Central

Barillà, Daniela; Carmelo, Emma; Hayes, Finbarr

2007-01-01

The ParF protein of plasmid TP228 belongs to the ubiquitous superfamily of ParA ATPases that drive DNA segregation in bacteria. ATP-bound ParF polymerizes into multistranded filaments. The partner protein ParG is dimeric, consisting of C-termini that interweave into a ribbon–helix–helix domain contacting the centromeric DNA and unstructured N-termini. ParG stimulates ATP hydrolysis by ParF ≈30-fold. Here, we establish that the mobile tails of ParG are crucial for this enhancement and that arginine R19 within the tail is absolutely required for activation of ParF nucleotide hydrolysis. R19 is part of an arginine finger-like loop in ParG that is predicted to intercalate into the ParF nucleotide-binding pocket thereby promoting ATP hydrolysis. Significantly, mutations of R19 abrogated DNA segregation in vivo, proving that intracellular stimulation of ATP hydrolysis by ParG is a key regulatory process for partitioning. Furthermore, ParG bundles ParF-ATP filaments as well as promoting nucleotide-independent polymerization. The N-terminal flexible tail is required for both activities, because N-terminal ΔParG polypeptides are defective in both functions. Strikingly, the critical arginine finger-like residue R19 is dispensable for ParG-mediated remodeling of ParF polymers, revealing that the ParG N-terminal tail possesses two separable activities in the interplay with ParF: a catalytic function during ATP hydrolysis and a mechanical role in modulation of polymerization. We speculate that activation of nucleotide hydrolysis via an arginine finger loop may be a conserved, regulatory mechanism of ParA family members and their partner proteins, including ParA-ParB and Soj-Spo0J that mediate DNA segregation and MinD-MinE that determine septum localization. PMID:17261809

The tail of the ParG DNA segregation protein remodels ParF polymers and enhances ATP hydrolysis via an arginine finger-like motif.

PubMed

Barillà, Daniela; Carmelo, Emma; Hayes, Finbarr

2007-02-06

The ParF protein of plasmid TP228 belongs to the ubiquitous superfamily of ParA ATPases that drive DNA segregation in bacteria. ATP-bound ParF polymerizes into multistranded filaments. The partner protein ParG is dimeric, consisting of C-termini that interweave into a ribbon-helix-helix domain contacting the centromeric DNA and unstructured N-termini. ParG stimulates ATP hydrolysis by ParF approximately 30-fold. Here, we establish that the mobile tails of ParG are crucial for this enhancement and that arginine R19 within the tail is absolutely required for activation of ParF nucleotide hydrolysis. R19 is part of an arginine finger-like loop in ParG that is predicted to intercalate into the ParF nucleotide-binding pocket thereby promoting ATP hydrolysis. Significantly, mutations of R19 abrogated DNA segregation in vivo, proving that intracellular stimulation of ATP hydrolysis by ParG is a key regulatory process for partitioning. Furthermore, ParG bundles ParF-ATP filaments as well as promoting nucleotide-independent polymerization. The N-terminal flexible tail is required for both activities, because N-terminal DeltaParG polypeptides are defective in both functions. Strikingly, the critical arginine finger-like residue R19 is dispensable for ParG-mediated remodeling of ParF polymers, revealing that the ParG N-terminal tail possesses two separable activities in the interplay with ParF: a catalytic function during ATP hydrolysis and a mechanical role in modulation of polymerization. We speculate that activation of nucleotide hydrolysis via an arginine finger loop may be a conserved, regulatory mechanism of ParA family members and their partner proteins, including ParA-ParB and Soj-Spo0J that mediate DNA segregation and MinD-MinE that determine septum localization.

A Langevin approach to multi-scale modeling

DOE PAGES

Hirvijoki, Eero

2018-04-13

In plasmas, distribution functions often demonstrate long anisotropic tails or otherwise significant deviations from local Maxwellians. The tails, especially if they are pulled out from the bulk, pose a serious challenge for numerical simulations as resolving both the bulk and the tail on the same mesh is often challenging. A multi-scale approach, providing evolution equations for the bulk and the tail individually, could offer a resolution in the sense that both populations could be treated on separate meshes or different reduction techniques applied to the bulk and the tail population. In this paper, we propose a multi-scale method which allowsmore » us to split a distribution function into a bulk and a tail so that both populations remain genuine, non-negative distribution functions and may carry density, momentum, and energy. The proposed method is based on the observation that the motion of an individual test particle in a plasma obeys a stochastic differential equation, also referred to as a Langevin equation. Finally, this allows us to define transition probabilities between the bulk and the tail and to provide evolution equations for both populations separately.« less

Research investigation of helicopter main rotor/tail rotor interaction noise

NASA Technical Reports Server (NTRS)

Fitzgerald, J.; Kohlhepp, F.

1988-01-01

Acoustic measurements were obtained in a Langley 14 x 22 foot Subsonic Wind Tunnel to study the aeroacoustic interaction of 1/5th scale main rotor, tail rotor, and fuselage models. An extensive aeroacoustic data base was acquired for main rotor, tail rotor, fuselage aerodynamic interaction for moderate forward speed flight conditions. The details of the rotor models, experimental design and procedure, aerodynamic and acoustic data acquisition and reduction are presented. The model was initially operated in trim for selected fuselage angle of attack, main rotor tip-path-plane angle, and main rotor thrust combinations. The effects of repositioning the tail rotor in the main rotor wake and the corresponding tail rotor countertorque requirements were determined. Each rotor was subsequently tested in isolation at the thrust and angle of attack combinations for trim. The acoustic data indicated that the noise was primarily dominated by the main rotor, especially for moderate speed main rotor blade-vortex interaction conditions. The tail rotor noise increased when the main rotor was removed indicating that tail rotor inflow was improved with the main rotor present.

A Langevin approach to multi-scale modeling

NASA Astrophysics Data System (ADS)

Hirvijoki, Eero

2018-04-01

In plasmas, distribution functions often demonstrate long anisotropic tails or otherwise significant deviations from local Maxwellians. The tails, especially if they are pulled out from the bulk, pose a serious challenge for numerical simulations as resolving both the bulk and the tail on the same mesh is often challenging. A multi-scale approach, providing evolution equations for the bulk and the tail individually, could offer a resolution in the sense that both populations could be treated on separate meshes or different reduction techniques applied to the bulk and the tail population. In this letter, we propose a multi-scale method which allows us to split a distribution function into a bulk and a tail so that both populations remain genuine, non-negative distribution functions and may carry density, momentum, and energy. The proposed method is based on the observation that the motion of an individual test particle in a plasma obeys a stochastic differential equation, also referred to as a Langevin equation. This allows us to define transition probabilities between the bulk and the tail and to provide evolution equations for both populations separately.

A Langevin approach to multi-scale modeling

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hirvijoki, Eero

In plasmas, distribution functions often demonstrate long anisotropic tails or otherwise significant deviations from local Maxwellians. The tails, especially if they are pulled out from the bulk, pose a serious challenge for numerical simulations as resolving both the bulk and the tail on the same mesh is often challenging. A multi-scale approach, providing evolution equations for the bulk and the tail individually, could offer a resolution in the sense that both populations could be treated on separate meshes or different reduction techniques applied to the bulk and the tail population. In this paper, we propose a multi-scale method which allowsmore » us to split a distribution function into a bulk and a tail so that both populations remain genuine, non-negative distribution functions and may carry density, momentum, and energy. The proposed method is based on the observation that the motion of an individual test particle in a plasma obeys a stochastic differential equation, also referred to as a Langevin equation. Finally, this allows us to define transition probabilities between the bulk and the tail and to provide evolution equations for both populations separately.« less

Utility of capillary microsampling for rat pharmacokinetic studies: Comparison of tail-vein bleed to jugular vein cannula sampling.

PubMed

Korfmacher, Walter; Luo, Yongyi; Ho, Stacy; Sun, Wei; Shen, Liduo; Wang, Jie; Wu, Zhongtao; Guo, Yang; Snow, Gregory; O'Shea, Thomas

2015-01-01

Serial sampling methods have been used for rat pharmacokinetic (PK) studies for over 20 years. Currently, it is still common to take 200-250 μL of blood at each timepoint when performing a PK study in rats and using serial sampling. While several techniques have been employed for collecting blood samples from rats, there is only limited published data to compare these methods. Recently, microsampling (≤ 50 μL) techniques have been reported as an alternative process for collecting blood samples from rats. In this report, five compounds were dosed orally into rats. For three proprietary compounds, jugular vein cannula (JVC) sampling was used to collect whole blood and plasma samples and capillary microsampling (CMS) was used to collect blood samples from the tail vein of the same animal. For the two other compounds, marketed drugs fluoxetine and glipizide, JVC sampling was used to collect both whole blood and blood CMS samples while tail-vein sampling from the same rats was also used to collect both whole blood and blood CMS samples. For the three proprietary compounds, the blood AUC as well as the blood concentration-time profile that were obtained from the tail vein were different from those obtained via JVC sampling. For fluoxetine, the blood total exposure (AUC) was not statistically different when comparing tail-vein sampling to JVC sampling, however the blood concentration-time profile that was obtained from the tail vein was different than the one obtained from JVC sampling. For glipizide, the blood AUC and concentration-time profile were not statistically different when comparing the tail-vein sampling to the JVC sampling. For both fluoxetine and glipizide, the blood concentration profiles obtained from CMS were equivalent to the blood concentration profiles obtained from the standard whole blood sampling, collected at the same sampling site. The data in this report provide strong evidence that blood CMS is a valuable small volume blood sampling approach for rats and that it provides results for test compound concentrations that are equivalent to those obtained from traditional whole blood sampling. The data also suggest that for some compounds, the concentration-time profile that is obtained for a test compound based on sampling from a rat tail vein may be different from that obtained from rat JVC sampling. In some cases, this shift in the concentration-time profile will result in different PK parameters for the test compound. Based on these observations, it is recommended that a consistent blood sampling method should be used for serial microsampling in discovery rat PK studies when testing multiple new chemical entities. If the rat tail vein sampling method is selected for PK screening, then conducting a bridging study on the lead compound is recommended to confirm that the rat PK obtained from JVC sampling is comparable to the tail-vein sampling. Copyright © 2015 Elsevier Inc. All rights reserved.

Spatial Characteristics of F/A-18 Vertical Tail Buffet Pressures Measured in Flight

NASA Technical Reports Server (NTRS)

Moses, Robert W.; Shah, Gautam H.

1998-01-01

Buffeting is an aeroelastic phenomenon which plagues high performance aircraft, especially those with twin vertical tails, at high angles of attack. Previous wind-tunnel and flight tests were conducted to characterize the buffet loads on the vertical tails by measuring surface pressures, bending moments, and accelerations. Following these tests, buffeting estimates were computed using the measured buffet pressures and compared to the measured responses. The estimates did not match the measured data because the assumed spatial correlation of the buffet pressures was not correct. A better understanding of the partial (spatial) correlation of the differential buffet pressures on the tail was necessary to improve the buffeting estimates. Several wind-tunnel investigations were conducted for this purpose. When combined and compared, the results of these tests show that the partial correlation depends on and scales with flight conditions. One of the remaining questions is whether the windtunnel data is consistent with flight data. Presented herein, cross-spectra and coherence functions calculated from pressures that were measured on the high alpha research vehicle (HARV) indicate that the partial correlation of the buffet pressures in flight agrees with the partial correlation observed in the wind tunnel.

Amitriptyline converts non-responders into responders to low-frequency electroacupuncture-induced analgesia in rats.

PubMed

Fais, Rafael S; Reis, G M; Rossaneis, A C; Silveira, J W S; Dias, Q M; Prado, W A

2012-07-26

The purpose of this study was to examine whether the use of intraperitoneal or intrathecal amitriptyline combined with electroacupuncture modifies the tail-flick reflex and incision pain in rats that normally do not have analgesia to electroacupuncture in the tail-flick test (non-responder rats). Changes in the nociceptive threshold of intraperitoneal or intrathecal saline- or amitriptyline-treated non-responder rats were evaluated using the tail-flick or incision pain tests before, during and after a 20-min period of electroacupuncture, applied at 2 Hz to the Zusanli and Sanynjiao acupoints. Amitriptyline was used at doses of 0.8 mg/kg or 30 μg/kg by intraperitoneal or intrathecal route, respectively. At these doses, amitriptyline has no effect against thermal or incision pain in rats. Rats selected as non-responders to the analgesic effect of electroacupuncture 2 Hz in tail-flick and incision pain tests become responders after an intraperitoneal or intrathecal injection of amitriptyline. Amitriptyline converts non-responder rats to rats that respond to electroacupuncture with analgesia in a model of thermal phasic pain and anti-hyperalgesia in a model of incision pain. Copyright © 2012 Elsevier Inc. All rights reserved.

Review: Observations of recent comets, ion tails

NASA Technical Reports Server (NTRS)

Brandt, J. C.

1976-01-01

Photographic plates of the moving structures in the cometary tail are examined. Several divergent explanations for the case of comet Kohoutek are presented. It is suggested that these hypotheses be tested by observing the motion of the material spectroscopically by means of the Doppler effect.

Hypersonic shock tunnel heat transfer tests of the Space Shuttle SILTS pod configuration

NASA Technical Reports Server (NTRS)

Wittliff, C. E.

1983-01-01

Heat transfer measurements have been made on a 0.0175-scale NASA Space Shuttle orbiter model having a simulated SILTS (Shuttle Infrared Leeside Temperature Sensor) pod on top of the vertical tail. Heat transfer distributions were measured both on the pod and on the vertical tail. The test program covered Mach numbers of 8, 11 and 16 in air, at Reynolds numbers from 100,000 to 18 million, based on model length. The angle of attack ranged from 30 deg to 40 deg at sideslip angles from -2 to +2 deg. Data were obtained with 92 thin film assistance thermometers located on the SILTS pod and on the upper 30 percent of the vertical tail. Heat transfer rates measured on the vertical tail show good agreement with flight data obtained from missions STS-1, -2 and -3. The variation of heat transfer to the pod with Reynolds number, Mach number and angle of attack is discussed.

Force Tests of a 1/5-Scale Model of the McDonnell XP-85 Airplane with Conventional Tail Assembly in the Langley Free-Flight Tunnel

NASA Technical Reports Server (NTRS)

Paulson, John W.; Johnson, Joseph L.

1947-01-01

At the request of the Air Materiel Command, Army Air Forces an investigation of the low-speed, power-off stability and control characteristics of the McDonnell XP-85 airplane is being conducted in the Langley free-flight tunnel. The XP-85 airplane is a parasite fighter carried in a bomb bay of the B-36 airplane. As a part of the investigation a few force tests were made of a 1/5 scale model of the XP-85 with a conventional tail assembly installed in place of the original design five-unit tail assembly. The total area of the conventional assembly was approximately 80 percent of the area of the five-unit assembly. The results of this investigation showed that the conventional tail assembly gave about the same longitudinal stability characteristics as the original configuration and improved the directional and lateral stability.

Development and flight evaluation of an augmented stability active controls concept with a small tail

NASA Technical Reports Server (NTRS)

1980-01-01

Parasite drag reduction evaluation is composed of wind tunnel tests with a standard L-1011 tail and two reduced area tail configurations. Trim drag reduction is evaluated by rebalancing the airplane for relaxed static stability. This is accomplished by pumping water to tanks in the forward and aft of the airplane to acheive desired center of gravity location. Also, the L-1011 is modified to incorporate term and advanced augmented systems. By using advanced wings and aircraft relaxed static stability significant fuel savings can be realized. An airplane's dynamic stability becomes more sensitive for decreased tail size, relaxed static stability, and advanced wing configurations. Active control pitch augmentation will be used to acheive the required handling qualities. Flight tests will be performed to evaluate the pitch augmentation systems. The effect of elevator downrig on stabilizer/elevator hinge moments will be measured. For control system analysis, the normal acceleration feedback and pitch rate feedback are analyzed.

The X-31A quasi-tailless flight test results

NASA Technical Reports Server (NTRS)

Bosworth, John T.; Stoliker, P. C.

1996-01-01

A quasi-tailless flight investigation was launched using the X-31A enhanced fighter maneuverability airplane. In-flight simulations were used to assess the effect of partial to total vertical tail removal. The rudder control surface was used to cancel the stabilizing effects of the vertical tail, and yaw thrust vector commands were used to restabilize and control the airplane. The quasi-tailless mode was flown supersonically with gentle maneuvering and subsonically in precision approaches and ground attack profiles. Pilot ratings and a full set of flight test measurements were recorded. This report describes the results obtained and emphasizes the lessons learned from the X-31A flight test experiment. Sensor-related issues and their importance to a quasi-tailless simulation and to ultimately controlling a directionally unstable vehicle are assessed. The X-31A quasi-tailless flight test experiment showed that tailless and reduced tail fighter aircraft are definitely feasible. When the capability is designed into the airplane from the beginning, the benefits have the potential to outweigh the added complexity required.

Diagnostic accuracy of rectal mucosa biopsy testing for chronic wasting disease within white-tailed deer (Odocoileus virginianus) herds in North America:Effects of age,sex,polymorphism at PRNP codon 96,and disease progression

USDA-ARS?s Scientific Manuscript database

An effective live animal diagnostic test is needed to assist in the control of chronic wasting disease (CWD), which has spread through captive and wild herds of white-tailed deer (Odocoileus virginianus) in Canada and the United States. In the present study, the diagnostic accuracy of rectal mucosa ...

Supersonic aerodynamic characteristics of a maneuvering canard-controlled missile with fixed and free-rolling tail fins

NASA Technical Reports Server (NTRS)

Blair, A. B., Jr.

1990-01-01

Wind tunnel investigations were conducted on a generic cruciform canard-controlled missile configuration. The model featured fixed or free-rolling tail-fin afterbodies to provide an expanded aerodynamic data base with particular emphasis on alleviating large induced rolling moments and/or for providing canard roll control throughout the entire test angle-of-attack range. The tests were conducted in the NASA Langley Unitary Plan Wind Tunnel at Mach numbers from 2.50 to 3.50 at a constant Reynolds number per foot of 2.00 x 10 to the 6th. Selected test results are presented to show the effects of a fixed or free-rolling tail-fin afterbody on the static longitudinal and lateral-directional aerodynamic characteristics of a canard-controlled missile with pitch, yaw, and roll control at model roll angles of 0 deg and 45 deg.

In Vivo Pet Imaging of Myelin Damage and Repair in the Spinal Cord

DTIC Science & Technology

2011-10-01

test , CI 99%). (C) Comparison of the average radiance between the Plp- Akt-DD mice ( red ) and wild-type mice (blue) after deducting the vehicle signals... red ) at 0, 2, 4, 6, 8, 10, 15, 20, 25, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120 minutes (Pɘ.0001, two-tailed t- test , CI 99%). (B) The average...120 minutes (Pɘ.0001, two-tailed t- test , CI 99%). (C) The comparison of the average radiance between the shiverer mice (blue) and wild-type mice ( red

Performance and loads data from an outdoor hover test of a Lynx tail rotor

NASA Technical Reports Server (NTRS)

Signor, David B.; Yamauchi, Gloria K.; Smith, Charles A.; Hagen, Martin J.

1989-01-01

A Lynx tail rotor was tested in hover at the Outdoor Aerodynamic Research Facility at NASA Ames Research Center. The test objectives were to measure the isolated rotor performance to provide a baseline for subsequent testing, and to operate the rotor throughout the speed and collective envelope before testing in the NFAC 40- by 80-Foot Wind Tunnel. Rotor forces and blade bending moments were measured at ambient wind conditions from zero to 6.23 m/sec. The test envelope was limited to rotor speeds of 1550 to 1850 rpm and minus 13 deg to plus 20 deg of blade collective pitch. The isolated rotor performance and blade loads data are presented.

Wind-Tunnel Tests of the 1/9-Scale Model of the Curtiss XP-62 Airplane with Various Vertical Tail Arrangements

NASA Technical Reports Server (NTRS)

Wallace, Arthur R.; Recant, I.G.

1943-01-01

The effect of various vertical tail arrangements upon the stability and control characteristics of an XP-62 fighter model was investigated. Rudder-free yaw characteristics with take-off power and flaps deflected were satisfactory after dorsal fin modifications. Directional stability was obtained with all modified vertical tails. Satisfactory rudder effectiveness resulted partly because the dual-rotation propellers produced no asymmetric yawing moments. Pedal forces in sideslips were undesirably large but may be easily reduced.

Design, Simulation, Fabrication and Testing of a Bio-Inspired Amphibious Robot with Multiple Modes of Mobility

DTIC Science & Technology

2012-01-01

performance. Ob- stacle climbing using the tail is compared to results from a previous robot with a posterior body segment and body flexion joint. Actual...3. Mechanisms of Locomotion for Multi-Modal Mobility 3.1. Gate and Tail Design Demands of multi-modal locomotion motivated a quadruped design for...tail instead of a rear body segment simplifies waterproofing design requirements and adds stability both on land and in water. This new morphology is

Trunk-rotation flexibility in collegiate softball players with or without a history of shoulder or elbow injury.

PubMed

Aragon, Veronica J; Oyama, Sakiko; Oliaro, Scott M; Padua, Darin A; Myers, Joseph B

2012-01-01

Throwing is a whole-body motion that requires the transfer of momentum from the lower extremity to the upper extremity via the trunk. No research to date examines the association between a history of shoulder or elbow injury and trunk flexibility in overhead athletes. To determine if injury history and trunk-rotation flexibility are associated and to compare trunk-rotation flexibility measured using 3 clinical tests: half-kneeling rotation test with the bar in the back, half-kneeling rotation test with the bar in the front, and seated rotation test in softball position players with or without a history of shoulder or elbow injury. Cross-sectional design. University softball facilities. Sixty-five female National Collegiate Athletic Association Division I softball position players. Trunk-rotation flexibility was measured with 3 clinical tests. Recent injury history was obtained using a questionnaire and verified by the certified athletic trainer. Binomial regression models were used to determine if injury history was associated with flexibility categories (high, normal, or limited tertiles) for each of the 6 (3 tests × 2 directions) trunk-rotation flexibility measures. Trunk-rotation flexibility measures from 3 clinical tests were compared between participants with and without a history of shoulder or elbow injury using analysis-of-variance models. When measured using the half-kneeling rotation test with the bar in the back and the seated rotation test, injury history and forward trunk-rotation flexibility were associated. However, no mean group differences were seen in trunk-rotation flexibility between participants with and without a history of shoulder or elbow injury. Limited forward trunk-rotation flexibility may be a risk factor for shoulder or elbow injuries. However, further study is needed to confirm the study finding.

Sulfide tails management within the framework of sustainable development in mineral sand mines--the case study of Sierra Rutile Ltd.

PubMed

Kallon, Senesie B; Jabati, Ansu M; Samura, Alusine

2011-01-01

The study discussed here assessed Sierra Rutile Ltd.'s (SRLs) water-cover sulfide tails management method. Monthly and quarterly water samples from SRLs Sulfide Tails Pond (STP), Total Tails Pond (TTP), and the Titan Domestic Pond (TDP) were analyzed for 15 months. Results indicated acceptable quality for the STP. From Student's t-test analysis, it was found that the mean pH of the TTP was significantly lower than that of the TDP (p < .05). Results did not indicate pollution of the TDP by SRLs tailings management. The water-cover method significantly suppressed sulfide oxidation in the STP. Concerns to be addressed, however, include potential overtopping of the pond, water level fluctuations, and the need for periodic reinforcement of the tailings embankments. A dedicated environmental monitoring campaign that includes other proximate water bodies is suggested; this should inform timely mitigation of any environmental contamination and promote sound environmental and public health outcomes.

Use of centrifugal-gravity concentration for rejection of talc and recovery improvement in base-metal flotation

NASA Astrophysics Data System (ADS)

Klein, Bern; Altun, Naci Emre; Ghaffari, Hassan

2016-08-01

The possibility of using a centrifugal-gravity concentrator to reject Mg-bearing minerals and minimize metal losses in the flotation of base metals was evaluated. Sample characterization, batch scoping tests, pilot-scale tests, and regrind-flotation tests were conducted on a Ni flotation tailings stream. Batch tests revealed that the Mg grade decreased dramatically in the concentrate products. Pilot-scale testing of a continuous centrifugal concentrator (Knelson CVD6) on the flotation tailings revealed that a concentrate with a low mass yield, low Mg content, and high Ni upgrade ratio could be achieved. Under optimum conditions, a concentrate at 6.7% mass yield was obtained with 0.85% Ni grade at 12.9% Ni recovery and with a low Mg distribution (1.7%). Size partition curves demonstrated that the CVD also operated as a size classifier, enhancing the rejection of talc fines. Overall, the CVD was capable of rejecting Mg-bearing minerals. Moreover, an opportunity exists for the novel use of centrifugal-gravity concentration for scavenging flotation tailings and/or after comminution to minimize amount of Mg-bearing minerals reporting to flotation.

Lateral Stability Characteristics of a 1/8.33-Scale Powered Model of the Republic XF-12 Airplane

NASA Technical Reports Server (NTRS)

Pepper, Edward; Foster, Gerald V.

1947-01-01

The XF-12 airplane is a high-performance photo-reconnaissance aircraft designed for the Army Air Forces by the Republic Aviation Corporation. An investigation of a 1/8.33 - scale powered model was made in the Langley l9-foot pressure tunnel to obtain information relative to the aerodynamic design of the airplane. The model was tested with and without the original vertical tail. and with two revised tails. For the revised tail no. 1, the span of the original vertical .tail was increased about 15 percent and the portion of the vertical tail between the stabilizer and fuselage behind the rudder hinge line was allowed to deflect simultaneously with the main rudder. Revision no. 2 incorporated the increased span, but the lower rudder was locked in the neutral position. For all the tail arrangements investigated it was indicated that the airplane will possess positive effective dihedral and will be directionally stable regardless of flap or power condition. The rudder effectiveness is greater for the revised tails than for the original tail, but this is offset by the increase in directional stability caused by the revised tail. All the rudder arrangements appear inadequate in trimming out the resultant yawing moments at zero yaw in a take - off condition with the left-hand outboard propeller windmilling and the remaining engines developing take-off power.

Exploring the atomic structure and conformational flexibility of a 320 Å long engineered viral fiber using X-ray crystallography

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bhardwaj, Anshul; Casjens, Sherwood R.; Cingolani, Gino, E-mail: gino.cingolani@jefferson.edu

2014-02-01

This study presents the crystal structure of a ∼320 Å long protein fiber generated by in-frame extension of its repeated helical coiled-coil core. Protein fibers are widespread in nature, but only a limited number of high-resolution structures have been determined experimentally. Unlike globular proteins, fibers are usually recalcitrant to form three-dimensional crystals, preventing single-crystal X-ray diffraction analysis. In the absence of three-dimensional crystals, X-ray fiber diffraction is a powerful tool to determine the internal symmetry of a fiber, but it rarely yields atomic resolution structural information on complex protein fibers. An 85-residue-long minimal coiled-coil repeat unit (MiCRU) was previously identifiedmore » in the trimeric helical core of tail needle gp26, a fibrous protein emanating from the tail apparatus of the bacteriophage P22 virion. Here, evidence is provided that an MiCRU can be inserted in frame inside the gp26 helical core to generate a rationally extended fiber (gp26-2M) which, like gp26, retains a trimeric quaternary structure in solution. The 2.7 Å resolution crystal structure of this engineered fiber, which measures ∼320 Å in length and is only 20–35 Å wide, was determined. This structure, the longest for a trimeric protein fiber to be determined to such a high resolution, reveals the architecture of 22 consecutive trimerization heptads and provides a framework to decipher the structural determinants for protein fiber assembly, stability and flexibility.« less

A contribution to improve the calculation of the acid generating potential of mining wastes.

PubMed

Chopard, Aurélie; Benzaazoua, Mostafa; Bouzahzah, Hassan; Plante, Benoît; Marion, Philippe

2017-05-01

Mine wastes from sulfide-bearing ore extraction and processing are often stored at the surface of mine sites and could generate mine drainage. Prediction tests are completed to predict the water quality associated with the deposition of mining wastes. Static tests can quickly assess the acid-generating potential (AP) and the neutralization potential (NP). Whereas some studies recommend to take into account a mineral reactivity factor for the NP determination, the reactivity rates of acidifying minerals are not considered in the AP calculation. The aim of this study is to bring contribution to the improvement of the static test determination by adding kinetic factors in the AP determination. Eight sulfides (pyrite, Ni-pyrite, pyrrhotite, Ni-pyrrhotite, chalcopyrite, galena, sphalerite, arsenopyrite) and a sulfosalt (gersdorffite) were separately submitted to kinetic tests in modified weathering cells. This test was selected for its rapidity of results and for the low amount of material used, as it is somewhat difficult to obtain pure minerals samples. Five synthetic tailings were composed by mixing pure sulfides in various proportions and submitted to the same kinetic tests. The oxidation rates of synthetic tailings were compared with the weighted combined oxidation rates of individual pure sulfides. The oxidation rates of the synthetic tailings calculated from those of pure sulfides are within the same order of magnitude than those obtained through the kinetic experiments. The AP of synthetic tailings were calculated according to standard equations of the literature and compared with the new method. Copyright © 2017 Elsevier Ltd. All rights reserved.

Pongamia pinnata inoculated with Bradyrhizobium liaoningense PZHK1 shows potential for phytoremediation of mine tailings.

PubMed

Yu, Xiumei; Li, Yangxin; Li, Yanmei; Xu, Chaohua; Cui, Yongliang; Xiang, Quanju; Gu, Yunfu; Zhao, Ke; Zhang, Xiaoping; Penttinen, Petri; Chen, Qiang

2017-02-01

Mine tailings contain high concentrations of metal contaminants and only little nutrients, making the tailings barren for decades after the mining has been terminated. Effective phytoremediation of mine tailings calls for deep-rooted, metal accumulating, and soil fertility increasing plants with tolerance against harsh environmental conditions. We assessed the potential of the biofuel leguminous tree Pongamia pinnata inoculated with plant growth promoting rhizobia to remediate iron-vanadium-titanium oxide (V-Ti magnetite) mine tailing soil by pot experiment and in situ remediation test. A metal tolerant rhizobia strain PZHK1 was isolated from the tailing soil and identified as Bradyrhizobium liaoningense by phylogenetic analysis. Inoculation with PZHK1 increased the growth of P. pinnata both in V-Ti magnetite mine tailings and in Ni-contaminated soil. Furthermore, inoculation increased the metal accumulation capacity and superoxide dismutase activity of P. pinnata. The concentrations of Ni accumulated by inoculated plants were higher than the hyperaccumulator threshold. Inoculated P. pinnata accumulated high concentration of Fe, far exceeding the upper limit (1000 mg kg -1 ) of Fe in plant tissue. In summary, P. pinnata-B. liaoningense PZHK1 symbiosis showed potential to be applied as an effective phytoremediation technology for mine tailings and to produce biofuel feedstock on the marginal land.

Functional territories in primate substantia nigra pars reticulata separately signaling stable and flexible values

PubMed Central

Hikosaka, Okihide

2014-01-01

Gaze is strongly attracted to visual objects that have been associated with rewards. Key to this function is a basal ganglia circuit originating from the caudate nucleus (CD), mediated by the substantia nigra pars reticulata (SNr), and aiming at the superior colliculus (SC). Notably, subregions of CD encode values of visual objects differently: stably by CD tail [CD(T)] vs. flexibly by CD head [CD(H)]. Are the stable and flexible value signals processed separately throughout the CD-SNr-SC circuit? To answer this question, we identified SNr neurons by their inputs from CD and outputs to SC and examined their sensitivity to object values. The direct input from CD was identified by SNr neuron's inhibitory response to electrical stimulation of CD. We found that SNr neurons were separated into two groups: 1) neurons inhibited by CD(T) stimulation, located in the caudal-dorsal-lateral SNr (cdlSNr), and 2) neurons inhibited by CD(H) stimulation, located in the rostral-ventral-medial SNr (rvmSNr). Most of CD(T)-recipient SNr neurons encoded stable values, whereas CD(H)-recipient SNr neurons tended to encode flexible values. The output to SC was identified by SNr neuron's antidromic response to SC stimulation. Among the antidromically activated neurons, many encoded only stable values, while some encoded only flexible values. These results suggest that CD(T)-cdlSNr-SC circuit and CD(H)-rvmSNr-SC circuit transmit stable and flexible value signals, largely separately, to SC. The speed of signal transmission was faster through CD(T)-cdlSNr-SC circuit than through CD(H)-rvmSNr-SC circuit, which may reflect automatic and controlled gaze orienting guided by these circuits. PMID:25540224

Testing a molasses-based bait for oral vaccination of white-tailed deer (Odocoileus virginianus) against Mycobacterium bovis

USDA-ARS?s Scientific Manuscript database

White-tailed deer (Odocoileus virginianus) in Michigan, USA are wildlife reservoirs of bovine tuberculosis (bTB) with documented spread to cattle. In vaccine efficacy trials, Mycobacterium bovis bacillus Calmette Guerin (BCG) administered orally reduces colonization and bTB-associated lesions in whi...

Mapping eastern equine encephalitis virus risk for white-tailed deer in Michigan

PubMed Central

Downs, Joni A.; Hyzer, Garrett; Marion, Eric; Smith, Zachary J.; Kelen, Patrick Vander; Unnasch, Thomas R.

2015-01-01

Eastern equine encephalitis (EEE) is a mosquito-borne viral disease that is often fatal to humans and horses. Some species including white-tailed deer and passerine birds can survive infection with the EEE virus (EEEV) and develop antibodies that can be detected using laboratory techniques. In this way, collected serum samples from free ranging white-tailed deer can be used to monitor the presence of the virus in ecosystems. This study developed and tested a risk index model designed to predict EEEV activity in white-tailed deer in a three-county area of Michigan. The model evaluates EEEV risk on a continuous scale from 0.0 (no measurable risk) to 1.0 (highest possible risk). High risk habitats are identified as those preferred by white-tailed deer that are also located in close proximity to an abundance of wetlands and lowland forests, which support disease vectors and hosts. The model was developed based on relevant literature and was tested with known locations of infected deer that showed neurological symptoms. The risk index model accurately predicted the known locations, with the mean value for those sites equal to the 94th percentile of values in the study area. The risk map produced by the model could be used refine future EEEV monitoring efforts that use serum samples from free-ranging white-tailed deer to monitor viral activity. Alternatively, it could be used focus educational efforts targeted toward deer hunters that may have elevated risks of infection. PMID:26494931

A Summary of Revisions Applied to a Turbulence Response Analysis Method for Flexible Aircraft Configurations

NASA Technical Reports Server (NTRS)

Funk, Christie J.; Perry, Boyd, III; Silva, Walter A.; Newman, Brett

2014-01-01

A software program and associated methodology to study gust loading on aircraft exists for a classification of geometrically simplified flexible configurations. This program consists of a simple aircraft response model with two rigid and three flexible symmetric degrees-of - freedom and allows for the calculation of various airplane responses due to a discrete one-minus- cosine gust as well as continuous turbulence. Simplifications, assumptions, and opportunities for potential improvements pertaining to the existing software program are first identified, then a revised version of the original software tool is developed with improved methodology to include more complex geometries, additional excitation cases, and additional output data so as to provide a more useful and precise tool for gust load analysis. In order to improve the original software program to enhance usefulness, a wing control surface and horizontal tail control surface is added, an extended application of the discrete one-minus-cosine gust input is employed, a supplemental continuous turbulence spectrum is implemented, and a capability to animate the total vehicle deformation response to gust inputs is included. These revisions and enhancements are implemented and an analysis of the results is used to validate the modifications.

Methods for In-Flight Wing Shape Predictions of Highly Flexible Unmanned Aerial Vehicles: Formulation of Ko Displacement Theory

NASA Technical Reports Server (NTRS)

Ko, William L.; Fleischer, Van Tran

2010-01-01

The Ko displacement theory is formulated for a cantilever tubular wing spar under bending, torsion, and combined bending and torsion loading. The Ko displacement equations are expressed in terms of strains measured at multiple sensing stations equally spaced on the surface of the wing spar. The bending and distortion strain data can then be input to the displacement equations to calculate slopes, deflections, and cross-sectional twist angles of the wing spar at the strain-sensing stations for generating the deformed shapes of flexible aircraft wing spars. The displacement equations have been successfully validated for accuracy by finite-element analysis. The Ko displacement theory that has been formulated could also be applied to calculate the deformed shape of simple and tapered beams, plates, and tapered cantilever wing boxes. The Ko displacement theory and associated strain-sensing system (such as fiber optic sensors) form a powerful tool for in-flight deformation monitoring of flexible wings and tails, such as those often employed on unmanned aerial vehicles. Ultimately, the calculated displacement data can be visually displayed in real time to the ground-based pilot for monitoring the deformed shape of unmanned aerial vehicles during flight.

States of phage T3/T7 capsids: buoyant density centrifugation and cryo-EM.

PubMed

Serwer, Philip; Wright, Elena T; Demeler, Borries; Jiang, Wen

2018-04-01

Mature double-stranded DNA bacteriophages have capsids with symmetrical shells that typically resist disruption, as they must to survive in the wild. However, flexibility and associated dynamism assist function. We describe biochemistry-oriented procedures used to find previously obscure flexibility for capsids of the related phages, T3 and T7. The primary procedures are hydration-based buoyant density ultracentrifugation and purified particle-based cryo-electron microscopy (cryo-EM). We review the buoyant density centrifugation in detail. The mature, stable T3/T7 capsid is a shell flexibility-derived conversion product of an initially assembled procapsid (capsid I). During DNA packaging, capsid I expands and loses a scaffolding protein to form capsid II. The following are observations made with capsid II. (1) The in vivo DNA packaging of wild type T3 generates capsid II that has a slight (1.4%), cryo-EM-detected hyper-expansion relative to the mature phage capsid. (2) DNA packaging in some altered conditions generates more extensive hyper-expansion of capsid II, initially detected by hydration-based preparative buoyant density centrifugation in Nycodenz density gradients. (3) Capsid contraction sometimes occurs, e.g., during quantized leakage of DNA from mature T3 capsids without a tail.

Tail Service Mast Umbilical Arrival

NASA Image and Video Library

2016-08-02

A crane lowers the first Tail Service Mast Umbilical (TSMU) onto a test stand at the Launch Equipment Test Facility at NASA’s Kennedy Space Center in Florida. Two TSMUs will provide liquid propellants and power to the Space Launch System (SLS) rocket’s core stage engine. Both TSMUs will connect to the zero-level deck on the mobile launcher, providing fuel and electricity to the SLS rocket before it launches on Exploration Mission 1. The TSMU will undergo testing and validation at the LETF to verify it is functioning properly. The center’s Engineering Directorate and the Ground Systems Development and Operations Program are overseeing processing and testing of the umbilicals.

Use of photogrammetry as a means to assess hybrids of rhesus (Macaca mulatta) and long-tailed (M. fascicularis) macaques.

PubMed

Jadejaroen, Janya; Hamada, Yuzuru; Kawamoto, Yoshi; Malaivijitnond, Suchinda

2015-01-01

Rhesus (Macaca mulatta) and long-tailed (M. fascicularis) macaques are the most commonly used non-human primate models for biomedical research, but it is difficult to identify these two species in the hybrid zone (15-20°N). In this work, we used morphological values obtained via photogrammetry to assess hybrids of rhesus and long-tailed macaques at Khao Khieow Open Zoo (KKZ; 13°21'N, 101°06'E), eastern Thailand. Long-tailed and rhesus macaques have species-specific tail lengths and contrasts of their yellowish pelages. The accuracy and precision of the relative tail length (%RTL) and the contrast of the yellow hue (Cb*) of the pelage, as obtained from photographs, were compared with the corresponding direct measurements (morphometrics). The photogrammetric and morphometric measurements of %RTL and Cb* were highly significantly correlated (r = 0.989 and 0.980, p < 0.001), and there were no significant differences between the two datasets (t test, p = 0.13 and 0.41; n = 42 and 17 for %RTL and Cb*, respectively). The reproducibilities of the %RTL and Cb* measurements (calculated in the photogrammetric case by taking photographs of the same macaques in two different environments) were significantly correlated between the datasets (r = 0.983 and 0.914, p < 0.001 and 0.005), and there were no significant differences between the datasets (t test, p = 0.539 and 0.344; n = 30 each for %RTL and Cb*, respectively). The %RTL and Cb* data were combined with data on the crown and cheek hair patterns and sex skin reddening of the macaques, and this combined data set was then analyzed by multiple correspondence analysis and agglomerative hierarchical cluster analysis, leading to the categorization of the rhesus macaques, long-tailed macaques, and hybrids at KKZ into five groups. Thus, photogrammetry can be utilized to identify macaque species or hybrids when species identification relies mainly on tail length and pelage color.

Aerodynamic characteristics of a canard-controlled missile at Mach numbers of 0.8, 1.3, and 1.75. [in the Ames 6 by 6 foot wind tunnel

NASA Technical Reports Server (NTRS)

Kassner, D. L.; Wettlaufer, B.

1977-01-01

A typical missile model with nose-mounted canards and cruciform tail surfaces was tested in the Ames 6- by 6-Foot Wind Tunnel to determine the contributions of the component aerodynamic surfaces to the static aerodynamic characteristics at Mach numbers of 0.8, 1.3, and 1.75 and Reynolds number of 625,000 based on body diameter. Data were obtained at angles of attack ranging from 0 deg to 24 deg for various stages of model build-up (i.e., with and without canard and/or tail surfaces). In addition, two different sets of canards and tail surfaces were investigated. For the canard and tail arrangements investigated, the model was trimmable at angles of attack up to about 7 deg with canard deflections of about 10 deg. Also, the tail arrangements studied provided ample pitch stability.

Feasibility Studies on Pipeline Disposal of Concentrated Copper Tailings Slurry for Waste Minimization

NASA Astrophysics Data System (ADS)

Senapati, Pradipta Kumar; Mishra, Barada Kanta

2017-06-01

The conventional lean phase copper tailings slurry disposal systems create pollution all around the disposal area through seepage and flooding of waste slurry water. In order to reduce water consumption and minimize pollution, the pipeline disposal of these waste slurries at high solids concentrations may be considered as a viable option. The paper presents the rheological and pipeline flow characteristics of copper tailings samples in the solids concentration range of 65-72 % by weight. The tailings slurry indicated non-Newtonian behaviour at these solids concentrations and the rheological data were best fitted by Bingham plastic model. The influence of solids concentration on yield stress and plastic viscosity for the copper tailings samples were discussed. Using a high concentration test loop, pipeline experiments were conducted in a 50 mm nominal bore (NB) pipe by varying the pipe flow velocity from 1.5 to 3.5 m/s. A non-Newtonian Bingham plastic pressure drop model predicted the experimental data reasonably well for the concentrated tailings slurry. The pressure drop model was used for higher size pipes and the operating conditions for pipeline disposal of concentrated copper tailings slurry in a 200 mm NB pipe with respect to specific power consumption were discussed.

Acute effect of essential oil of Eugenia caryophyllata on cognition and pain in mice.

PubMed

Halder, Sumita; Mehta, Ashish K; Mediratta, Pramod K; Sharma, Krishna K

2012-06-01

The essential oil of Eugenia caryophyllata (clove oil; Family: Myrtaceae) is used in dental care as an antiseptic and analgesic. The study aims to evaluate the effect of clove oil on experimental models of pain and cognition in mice. To observe the acute effects of clove oil at different doses, the elevated plus maze was used for the assessment of cognition, and the tail flick and formalin tests were used for the study of pain. The formalin test showed that clove oil (0.1 ml/kg, i.p.) demonstrated significantly reduced pain response in both the phases. The lower doses (0.025 and 0.05 ml/kg, i.p.) reduced the formalin-induced pain response significantly in the second phase only. The tail-flick test showed variable response. The dose 0.1 ml/kg, clove oil, significantly decreased the tail-flick latency at 30 min and this effect was reversed by naloxone (1 mg/kg). On the contrary, the dose 0.025 ml/kg of clove oil, at 30 and 60 min increased the mean tail-flick latency compared to control group, but this effect was not statistically significant. Yet naloxone significantly (p < 0.05) reversed the effect of clove oil 0.025 ml/kg at 30 min. Clove oil (0.025 and 0.05 ml/kg, i.p.) significantly reversed the scopolamine-induced retention memory deficit induced by scopolamine, but clove oil (0.1 ml/kg, i.p.) significantly reversed both acquisition as well as retention deficits in elevated plus maze induced by the scopolamine. Clove oil exhibits reduced pain response by a predominantly peripheral action as evidenced by formalin test and the tail flick test showed the involvement of opioid receptors. Clove oil also significantly improved scopolamine-induced retention memory deficit at all doses.

Assessing Risks of Mine Tailing Dam Failures

NASA Astrophysics Data System (ADS)

Concha Larrauri, P.; Lall, U.

2017-12-01

The consequences of tailings dam failures can be catastrophic for communities and ecosystems in the vicinity of the dams. The failure of the Fundão tailings dam at the Samarco mine in 2015 killed 19 people with severe consequences for the environment. The financial and legal consequences of a tailings dam failure can also be significant for the mining companies. For the Fundão tailings dam, the company had to pay 6 billion dollars in fines and twenty-one executives were charged with qualified murder. There are tenths of thousands of active, inactive, and abandoned tailings dams in the world and there is a need to better understand the hazards posed by these structures to downstream populations and ecosystems. A challenge to assess the risks of tailings dams in a large scale is that many of them are not registered in publicly available databases and there is little information about their current physical state. Additionally, hazard classifications of tailings dams - common in many countries- tend to be subjective, include vague parameter definitions, and are not always updated over time. Here we present a simple methodology to assess and rank the exposure to tailings dams using ArcGIS that removes subjective interpretations. The method uses basic information such as current dam height, storage volume, topography, population, land use, and hydrological data. A hazard rating risk was developed to compare the potential extent of the damage across dams. This assessment provides a general overview of what in the vicinity of the tailings dams could be affected in case of a failure and a way to rank tailings dams that is directly linked to the exposure at any given time. One hundred tailings dams in Minas Gerais, Brazil were used for the test case. This ranking approach could inform the risk management strategy of the tailings dams within a company, and when disclosed, it could enable shareholders and the communities to make decisions on the risks they are taking.

Balancing Flexible Constraints and Measurement Precision in Computerized Adaptive Testing

ERIC Educational Resources Information Center

Moyer, Eric L.; Galindo, Jennifer L.; Dodd, Barbara G.

2012-01-01

Managing test specifications--both multiple nonstatistical constraints and flexibly defined constraints--has become an important part of designing item selection procedures for computerized adaptive tests (CATs) in achievement testing. This study compared the effectiveness of three procedures: constrained CAT, flexible modified constrained CAT,…

Heavy Tail Behavior of Rainfall Extremes across Germany

NASA Astrophysics Data System (ADS)

Castellarin, A.; Kreibich, H.; Vorogushyn, S.; Merz, B.

2017-12-01

Distributions are termed heavy-tailed if extreme values are more likely than would be predicted by probability distributions that have exponential asymptotic behavior. Heavy-tail behavior often leads to surprise, because historical observations can be a poor guide for the future. Heavy-tail behavior seems to be widespread for hydro-meteorological extremes, such as extreme rainfall and flood events. To date there have been only vague hints to explain under which conditions these extremes show heavy-tail behavior. We use an observational data set consisting of 11 climate variables at 1440 stations across Germany. This homogenized, gap-free data set covers 110 years (1901-2010) at daily resolution. We estimate the upper tail behavior, including its uncertainty interval, of daily precipitation extremes for the 1,440 stations at the annual and seasonal time scales. Different tail indicators are tested, including the shape parameter of the Generalized Extreme Value distribution, the upper tail ratio and the obesity index. In a further step, we explore to which extent the tail behavior can be explained by geographical and climate factors. A large number of characteristics is derived, such as station elevation, degree of continentality, aridity, measures for quantifying the variability of humidity and wind velocity, or event-triggering large-scale atmospheric situation. The link between the upper tail behavior and these characteristics is investigated via data mining methods capable of detecting non-linear relationships in large data sets. This exceptionally rich observational data set, in terms of number of stations, length of time series and number of explaining variables, allows insights into the upper tail behavior which is rarely possible given the typical observational data sets available.

Telling tails: selective pressures acting on investment in lizard tails.

PubMed

Fleming, Patricia A; Valentine, Leonie E; Bateman, Philip W

2013-01-01

Caudal autotomy is a common defense mechanism in lizards, where the animal may lose part or all of its tail to escape entrapment. Lizards show an immense variety in the degree of investment in a tail (i.e., length) across species, with tails of some species up to three or four times body length (snout-vent length [SVL]). Additionally, body size and form also vary dramatically, including variation in leg development and robustness and length of the body and tail. Autotomy is therefore likely to have fundamentally different effects on the overall body form and function in different species, which may be reflected directly in the incidence of lost/regenerating tails within populations or, over a longer period, in terms of relative tail length for different species. We recorded data (literature, museum specimens, field data) for relative tail length (n=350 species) and the incidence of lost/regenerating tails (n=246 species). We compared these (taking phylogeny into account) with intrinsic factors that have been proposed to influence selective pressures acting on caudal autotomy, including body form (robustness, body length, leg development, and tail specialization) and ecology (foraging behavior, physical and temporal niches), in an attempt to identify patterns that might reflect adaptive responses to these different factors. More gracile species have relatively longer tails (all 350 spp., P < 0.001; also significant for five of the six families tested separately), as do longer (all species, P < 0.001; Iguanidae, P < 0.05; Lacertidae, P < 0.001; Scindidae, P < 0.001), climbing (all species, P < 0.05), and diurnal (all species, P < 0.01; Pygopodidae, P < 0.01) species; geckos without specialized tails (P < 0.05); or active-foraging skinks (P < 0.05). We also found some relationships with the data for caudal autotomy, with more lost/regenerating tails for nocturnal lizards (all 246 spp., P < 0.01; Scindidae, P < 0.05), larger skinks (P < 0.05), climbing geckos (P < 0.05), or active-foraging iguanids (P < 0.05). The selective advantage of investing in a relatively longer tail may be due to locomotor mechanics, although the patterns observed are also largely consistent with predictions based on predation pressure.

Pressure-Distribution Measurements on the Tail Surfaces of a Rotating Model of the Design BFW - M31

NASA Technical Reports Server (NTRS)

Kohler, M.; Mautz, W.

1949-01-01

In order to obtain insight into the flow conditions on tail surfaces on airplanes during spins, pressure-distribution measurements were performed on a rotating model of the design BFW-M31. For the time being, the tests were made for only one angle of attack (alpha = 60 degrees) and various angles of yaw and rudder angles. The results of these measurements are given; the construction of the model, and the test arrangement used are described. Measurements to be performed later and alterations planned in the test arrangement are pointed out.

Space Shuttle Tail Service Mast Concept Verification

NASA Technical Reports Server (NTRS)

Uda, R. T.

1976-01-01

Design studies and analyses were performed to describe the loads and dynamics of the space shuttle tail service masts (TSMs). Of particular interest are the motion and interaction of the umbilical carrier plate, lanyard system, vacuum jacketed hoses, latches, links, and masthead. A development test rig was designed and fabricated to obtain experimental data. The test program is designed to (1) verify the theoretical dynamics calculations, (2) prove the soundness of design concepts, and (3) elucidate problem areas (if any) in the design of mechanisms and structural components. Design, fabrication, and initiation of TSM development testing at Kennedy Space Center are described.

Results of the AFRSI detailed-environment test of the 0.035-scale SSV pressure-loads model 84-0 in the Ames 11x11 ft. TWT and the Lewis 8x6 ft. and 10x10 ft. SWT (OA-310A, B, C), volume 1

NASA Technical Reports Server (NTRS)

Marshall, B. A.; Marroquin, J.

1984-01-01

Detailed orbiter aerodynamic and aeroacoustic pressure data were obtained in a three-part experimental investigation (OA-310A, B and C). The tests were conducted in three NASA facilities: OA-310A in the Ames 11x11-foot Transonic Wind Tunnel; OA-310B in the Lewis 8x6-foot Supersonic Wind Tunnel; and OA-310C in the Lewis 10x10-foot Supersonic Wind Tunnel. Test data were obtained to support analysis of the Space Transportation System (STS)-6 advanced flexible reusable surface insulation (AFRSI) anomaly using the 0.035-scale space shuttle vehicle pressure-loads Model 84-0. Data were obtained in the areas of the orbiter where AFRSI is to be applied to OV-099 and OV-103. Emphasis was placed on acquiring detailed aeroacoustic data and time-averaged pressure distributions on five affected areas: (1) canopy; (2) side of fuselage; (3) upper surface of wing; (4) OMS pods; and (5) vertical tail. Data were obtained at nominal ascent and entry atmospheric flight trajectory conditions between M=0.6 through M-3.5. Sample plotted data are given. aba M.G.

Tests of a Full-Scale Model of the Republic XF-91 Airplane in the Ames 40- by 80-Foot Wind Tunnel. Force and Moment Data

NASA Technical Reports Server (NTRS)

Hunten, Lynn W.; Dew, Joseph K.

1949-01-01

Wind-tunnel tests of a full-scale model of the Republic XF-91 airplane having swept-back wings and a vee tail were conducted to determine both the stability and control characteristics of the model longitudinally, laterally, and directionally. Configurations of the model were investigated involving such variables as external fuel tanks, a landing gear, trailing-edge flaps, leading-edge slats, and a range of wing incidences and tail incidences.

These two NASA F/A-18 aircraft are flying a test point for the Autonomous Formation Flight project o

NASA Technical Reports Server (NTRS)

2001-01-01

Two NASA F/A-18 aircraft are flying a test point for the Autonomous Formation Flight project over California's Mojave Desert. This second flight phase is mapping the wingtip vortex of the lead aircraft, the Systems Research Aircraft (tail number 847), on the trailing F/A-18 tail number 847. Wingtip vortex is a spiraling wind flowing from the wing during flight. The project is studying the drag and fuel reduction of precision formation flying.

Tree-Substrate Water Relations and Root Development in Tree Plantations Used for Mine Tailings Reclamation.

PubMed

Guittonny-Larchevêque, Marie; Bussière, Bruno; Pednault, Carl

2016-05-01

Tree water uptake relies on well-developed root systems. However, mine wastes can restrict root growth, in particular metalliferous mill tailings, which consist of the finely crushed ore that remains after valuable metals are removed. Thus, water stress could limit plantation success in reclaimed mine lands. This study evaluates the effect of substrates varying in quality (topsoil, overburden, compost and tailings mixture, and tailings alone) and quantity (50- or 20-cm-thick topsoil layer vs. 1-m plantation holes) on root development and water stress exposure of trees planted in low-sulfide mine tailings under boreal conditions. A field experiment was conducted over 2 yr with two tree species: basket willow ( L.) and hybrid poplar ( Moench × A. Henry). Trees developed roots in the tailings underlying the soil treatments despite tailings' low macroporosity. However, almost no root development occurred in tailings underlying a compost and tailings mixture. Because root development and associated water uptake was not limited to the soil, soil volume influenced neither short-term (water potential and instantaneous transpiration) nor long-term (δC) water stress exposure in trees. However, trees were larger and had greater total leaf area when grown in thicker topsoil. Despite a volumetric water content that always remained above permanent wilting point in the tailings colonized by tree roots, measured foliar water potentials at midday were lower than drought thresholds reported for both tested tree species. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

Trunk-Rotation Flexibility in Collegiate Softball Players With or Without a History of Shoulder or Elbow Injury

PubMed Central

Aragon, Veronica J.; Oyama, Sakiko; Oliaro, Scott M.; Padua, Darin A.; Myers, Joseph B.

2012-01-01

Context: Throwing is a whole-body motion that requires the transfer of momentum from the lower extremity to the upper extremity via the trunk. No research to date examines the association between a history of shoulder or elbow injury and trunk flexibility in overhead athletes. Objective: To determine if injury history and trunk-rotation flexibility are associated and to compare trunk-rotation flexibility measured using 3 clinical tests: half-kneeling rotation test with the bar in the back, half-kneeling rotation test with the bar in the front, and seated rotation test in softball position players with or without a history of shoulder or elbow injury. Design: Cross-sectional design. Setting: University softball facilities. Patients or Other Participants: Sixty-five female National Collegiate Athletic Association Division I softball position players. Intervention(s): Trunk-rotation flexibility was measured with 3 clinical tests. Recent injury history was obtained using a questionnaire and verified by the certified athletic trainer. Main Outcome Measure(s): Binomial regression models were used to determine if injury history was associated with flexibility categories (high, normal, or limited tertiles) for each of the 6 (3 tests × 2 directions) trunk-rotation flexibility measures. Trunk-rotation flexibility measures from 3 clinical tests were compared between participants with and without a history of shoulder or elbow injury using analysis-of-variance models. Results: When measured using the half-kneeling rotation test with the bar in the back and the seated rotation test, injury history and forward trunk-rotation flexibility were associated. However, no mean group differences were seen in trunk-rotation flexibility between participants with and without a history of shoulder or elbow injury. Conclusions: Limited forward trunk-rotation flexibility may be a risk factor for shoulder or elbow injuries. However, further study is needed to confirm the study finding. PMID:23068587

Systemic synergism between codeine and morphine in three pain models in mice.

PubMed

Miranda, Hugo F; Noriega, Viviana; Zepeda, Ramiro J; Sierralta, Fernando; Prieto, Juan C

2013-01-01

The combination of two analgesic agents offers advantages in pain treatment. Codeine and morphine analgesia is due to activation of opioid receptor subtypes. This study, performed in mice using isobolographic analysis, evaluated the type of interaction in intraperitoneal (ip) or intrathecal (it) coadministration of codeine and morphine, in three nociceptive behavioral models. Intrathecal morphine resulted to be 7.5 times more potent than ip morphine in the writhing test, 55.6 times in the tail flick test and 1.7 times in phase II of the orofacial formalin test; however, in phase I of the same test ip was 1.2 times more potent than it morphine. Intrathecal codeine resulted being 3.4 times more potent than ip codeine in the writhing test, 1.6 times in the tail flick test, 2.5 times in phase I and 6.7 times in phase II of the orofacial formalin test. Opioid coadministration had a synergistic effect in the acute tonic pain (acetic acid writhing test), acute phasic pain (tail flick test) and inflammatory pain (orofacial formalin test). The interaction index ranged between 0.284 (writhing ip) and 0.440 (orofacial formalin phase II ip). This synergy may relate to the different pathways of pain transmission and to the different intracellular signal transduction. The present findings also raise the possibility of potential clinical advantages in combining opioids in pain management.

Charts for Estimating Tail-rotor Contribution to Helicopter Directional Stability and Control in Low-Speed Flight

NASA Technical Reports Server (NTRS)

Amer, Kenneth B; Gessow, Alfred

1955-01-01

Theoretically derived charts and equations are presented by which tail-rotor design studies of directional trim and control response at low forward speed can be conveniently made. The charts can also be used to obtain the main-rotor stability derivatives of thrust with respect to collective pitch and angle of attack at low forward speeds. The use of the charts and equations for tail-rotor design studies is illustrated. Comparisons between theoretical and experimental results are presented. The charts indicate, and flight tests confirm, that the region of vortex roughness which is familiar for the main rotor is also encountered by the tail rotor and that prolonged operation at the corresponding flight conditions would be difficult.

Measurements of atmospheric turbulence effects on tail rotor acoustics

NASA Technical Reports Server (NTRS)

Hagen, Martin J.; Yamauchi, Gloria K.; Signor, David B.; Mosher, Marianne

1994-01-01

Results from an outdoor hover test of a full-scale Lynx tail rotor are presented. The investigation was designed to further the understanding of the acoustics of an isolated tail rotor hovering out-of-ground effect in atmospheric turbulence, without the effects of the main rotor wake or other helicopter components. Measurements include simultaneous rotor performance, noise, inflow, and far-field atmospheric turbulence. Results with grid-generated inflow turbulence are also presented. The effects of atmospheric turbulence ingestion on rotor noise are quantified. In contradiction to current theories, increasing rotor inflow and rotor thrust were found to increase turbulence ingestion noise. This is the final report of Task 13A--Helicopter Tail Rotor Noise, of the NASA/United Kingdom Defense Research Agency cooperative Aeronautics Research Program.

Use of cemented paste backfill in arsenic-rich tailings

NASA Astrophysics Data System (ADS)

Hamberg, Roger; Maurice, Christian; Alakangas, Lena

2015-04-01

Gold is extracted by cyanide leaching from inclusions in arsenopyrite from a mine in the north of Sweden. The major ore mineral assemblage consists of pyrrhotite and arsenopyrite-loellingite. Effluents from the gold extraction were treated with Fe2(SO4)3, with the aim to form stable As-bearing Fe-precipitates (FEP). The use of the method called cemented paste backfill (CPB) is sometimes suggested for the management of tailings. In CPB, tailings are commonly mixed with low proportions (3 - 7 %) of cement and backfilled into underground excavated area. To reduce costs, amendments such as granulated blast furnace slag (GBFS), biofuel fly ash (BFA) and cement kiln dust (CKD) are used for partial replacement of cement in CPB due to their pozzolanic and alkaline properties. The objective for this study was to evaluate the leaching behaviour of As in CPB-mixtures with low proportions (1 - 3 %) of BFA and ordinary cement and unmodified tailings. The selection of CPB-recipies was made based on technical and economical criterias to adress the demands deriving from the mining operations. Speciation of the As in ore and tailings samples revealed that mining processes have dissolved the majority of the arsenopyrite in the ore, causing secondary As phases to co-precipitate with newly formed FEP:s. Tank leaching tests (TLT) and weathering cells (WCT) were used to compare leaching behaviour in a monolithic mass contra a crushed material. Quantification of the presumed benefit of CPB was made by calculation of the cumulative leaching of As. Results from the leaching tests (TLT and WCT) showed that the inclusion of As-rich tailings into a cementitious matrix increased leaching of As. This behaviour could partially be explained by an increase of pH. The addition of alkaline binder materials to tailings increased As leaching due to the relocation of desorbed As from FEPs into less acid-tolerant species such as Ca-arsenates and cementitious As-phases. Unmodified tailings generated an acidic environment in which As-bearing FEPs were stable. The addition of binders increased the tailings' acid-neutralizing capacity and introduced more Ca-ions and Fe-precipitates into the tailings matrix, both of which may facilitate As adsorption and reduce the potential for sulphide oxidation on a long-term basis.

Molecular modelling of protein-protein/protein-solvent interactions

NASA Astrophysics Data System (ADS)

Luchko, Tyler

The inner workings of individual cells are based on intricate networks of protein-protein interactions. However, each of these individual protein interactions requires a complex physical interaction between proteins and their aqueous environment at the atomic scale. In this thesis, molecular dynamics simulations are used in three theoretical studies to gain insight at the atomic scale about protein hydration, protein structure and tubulin-tubulin (protein-protein) interactions, as found in microtubules. Also presented, in a fourth project, is a molecular model of solvation coupled with the Amber molecular modelling package, to facilitate further studies without the need of explicitly modelled water. Basic properties of a minimally solvated protein were calculated through an extended study of myoglobin hydration with explicit solvent, directly investigating water and protein polarization. Results indicate a close correlation between polarization of both water and protein and the onset of protein function. The methodology of explicit solvent molecular dynamics was further used to study tubulin and microtubules. Extensive conformational sampling of the carboxy-terminal tails of 8-tubulin was performed via replica exchange molecular dynamics, allowing the characterisation of the flexibility, secondary structure and binding domains of the C-terminal tails through statistical analysis methods. Mechanical properties of tubulin and microtubules were calculated with adaptive biasing force molecular dynamics. The function of the M-loop in microtubule stability was demonstrated in these simulations. The flexibility of this loop allowed constant contacts between the protofilaments to be maintained during simulations while the smooth deformation provided a spring-like restoring force. Additionally, calculating the free energy profile between the straight and bent tubulin configurations was used to test the proposed conformational change in tubulin, thought to cause microtubule destabilization. No conformational change was observed but a nucleotide dependent 'softening' of the interaction was found instead, suggesting that an entropic force in a microtubule configuration could be the mechanism of microtubule collapse. Finally, to overcome much of the computational costs associated with explicit soIvent calculations, a new combination of molecular dynamics with the 3D-reference interaction site model (3D-RISM) of solvation was integrated into the Amber molecular dynamics package. Our implementation of 3D-RISM shows excellent agreement with explicit solvent free energy calculations. Several optimisation techniques, including a new multiple time step method, provide a nearly 100 fold performance increase, giving similar computational performance to explicit solvent.

Chronic wasting disease in free-ranging Wisconsin white-tailed deer

USGS Publications Warehouse

Joly, D.O.; Ribic, C.A.; Langenberg, J.A.; Beheler, K.; Batha, C.A.; Dhuey, B.J.; Rolley, R.E.; Bartelt, G.; VanDeelen, T.R.; Samuel, M.D.

2003-01-01

Three White-tailed Deer shot within 5 km during the 2001 hunting season in Wisconsin tested positive for chronic wasting disease, a prion disease of cervids. Subsequent sampling within 18 km showed a 3% prevalence (n=476). This discovery represents an important range extension for chronic wasting disease into the eastern United States.

Removal of heavy metals from Missouri lead mill tailings by froth flotation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Benn, F.W.; Cornell, W.L.

Froth flotation techniques to remove heavy metals (Pb, Cu, and Zn) from southeast Missouri lead mill tailings were investigated. It has been estimated that southeast Missouri contains between 200 and 300 million st of Pb tailings stored above ground. The tailings were classified as two distinct types: (1) pre-1968 tailings from the Old Lead Belt (some more than 100 years old) and (2) post-1968 tailings from the New Lead Belt. The objectives of the investigation were to reduce the Pb remaining in the tailings to < 500 ppm (< 0.05 pct Pb) and to attempt to recover a marketable concentratemore » to offset a portion of the remediation costs. The remaining dolomite-limestone would then be used as mining backfill or agricultural limestone. Bench-scale froth flotation removed, in percent, 95 Pb, 84 Cu, and 54 Zn, leaving 94 pct of the original weight containing, in parts per million, 400 Pb, 40 Cu, and 300 Zn from the Old Lead Belt tailings. Separate flotation tests also removed, in percent, 85 Pb, 84 Cu, and 80 Zn, leaving 75 pct of the original weight containing, in parts per million, 400 Pb, 200 Cu, and 500 Zn from the New Lead Belt tailings. Concentrates recovered from the Old Lead Belt were retreated to produce a final Pb concentrate containing 72 pct Pb with a cleaner flotation recovery of 79 pct. Froth flotation proved to be a viable method to remove the heavy metals.« less

Two-Dimensional Fourier Transform Applied to Helicopter Flyover Noise

NASA Technical Reports Server (NTRS)

Santa Maria, Odilyn L.

1999-01-01

A method to separate main rotor and tail rotor noise from a helicopter in flight is explored. Being the sum of two periodic signals of disproportionate, or incommensurate frequencies, helicopter noise is neither periodic nor stationary, but possibly harmonizable. The single Fourier transform divides signal energy into frequency bins of equal size. Incommensurate frequencies are therefore not adequately represented by any one chosen data block size. A two-dimensional Fourier analysis method is used to show helicopter noise as harmonizable. The two-dimensional spectral analysis method is first applied to simulated signals. This initial analysis gives an idea of the characteristics of the two-dimensional autocorrelations and spectra. Data from a helicopter flight test is analyzed in two dimensions. The test aircraft are a Boeing MD902 Explorer (no tail rotor) and a Sikorsky S-76 (4-bladed tail rotor). The results show that the main rotor and tail rotor signals can indeed be separated in the two-dimensional Fourier transform spectrum. The separation occurs along the diagonals associated with the frequencies of interest. These diagonals are individual spectra containing only information related to one particular frequency.

Domestic cat

USGS Publications Warehouse

Diffendorfer, James E.

2017-01-01

The familiar domestic cat is not native to southern California and is considered an invasive spe-cies by biologists and conservation organizations. When owners abandon their cats, wild or feral populations may arise, as they have in San Diego County. Cats’ pelage color, tail length, and hair thickness vary widely, given human fascination with breeding diverse phenotypes, but all have a typical felid body with upright ears, forward-looking eyes adapted for nocturnal foraging, protractible claws, and a sinuous, flexible body. Cats allowed outdoors and feral cats kill and eat a wide variety of vertebrates such as small mammals, birds, and reptiles

Ground/Flight Correlation of Aerodynamic Loads with Structural Response

NASA Technical Reports Server (NTRS)

Mangalam, Arun S.; Davis, Mark C.

2009-01-01

Ground and flight tests provide a basis and methodology for in-flight characterization of the aerodynamic and structural performance through the monitoring of the fluid-structure interaction. The NF-15B flight tests of the Intelligent Flight Control System program provided a unique opportunity to test the correlation of aerodynamic loads with points of flow attaching and detaching from the surface, which are also known as flow bifurcation points, as observed in a previous wind tunnel test performed at the U.S. Air Force Academy (Colorado Springs, Colorado). Moreover, flight tests, along with the subsequent unsteady aerodynamic tests in the NASA Transonic Dynamics Tunnel (TDT), provide a basis using surface flow sensors as means of assessing the aeroelastic performance of flight vehicles. For the flight tests, the NF-15B tail was instrumented with hot-film sensors and strain gages for measuring root-bending strains. This data were gathered via selected sideslip maneuvers performed at level flight and subsonic speeds. The aerodynamic loads generated by the sideslip maneuver resulted in a structural response, which were then compared with the hot-film sensor signals. The hot-film sensor signals near the stagnation region were found to be highly correlated with the root-bending strains. For the TDT tests, a flexible wing section developed under the U.S. Air Force Research Lab SensorCraft program was instrumented with strain gages, accelerometers, and hot-film sensors at two span stations. The TDT tests confirmed the correlation between flow bifurcation points and the wing structural response to tunnel-generated gusts. Furthermore, as the wings structural modes were excited by the gusts, a gradual phase change between the flow bifurcation point and the structural mode occurred during a resonant condition.

Effects of Caudal Elevation on Testicular Function in Rats: Separation of Effects on Spermatogenesis and Steroidogenesis

NASA Technical Reports Server (NTRS)

Deaver, D. R.; Amann, R. P.; Hammerstedt, R. H.; Ball, R.; Veeramachaneni, D. N. R.; Musacchia, X. J.

1992-01-01

A variety of biologic processes are perturbed when exposed to microgravity (space flight) for more than 7 days, including testicular function. Suspension of rats in a special harness (caudal elevation) to induce thoracic pooling of blood fluids and remove the support function of the hind limbs is used to mimic, on earth, the effects of microgravity encountered during space flight. Typically, this induces cryptorchidism in male rats. Three experiments were conducted to differentiate the effects of caudal elevation (30 deg angle) and anatomic location of testes on spermatogenesis and steroidogenesis. Rats were subjected to caudal elevation for 7 days using either a tail harness or a whole-body harness. Testes of rats fell into the abdominal cavity when a tail harness was used, but ligation of the iguinal canal prevented this repositioning. For rats with abdominal testes, testicular weight was reduced (P less than 0.05) and histology of testes was abnormal; the number of spermatids per gram parenchyma was lower (P less than 0.05) in tail-suspended rats compared with control rats.

Coupled nonlinear aeroelasticity and flight dynamics of fully flexible aircraft

NASA Astrophysics Data System (ADS)

Su, Weihua

This dissertation introduces an approach to effectively model and analyze the coupled nonlinear aeroelasticity and flight dynamics of highly flexible aircraft. A reduced-order, nonlinear, strain-based finite element framework is used, which is capable of assessing the fundamental impact of structural nonlinear effects in preliminary vehicle design and control synthesis. The cross-sectional stiffness and inertia properties of the wings are calculated along the wing span, and then incorporated into the one-dimensional nonlinear beam formulation. Finite-state unsteady subsonic aerodynamics is used to compute airloads along lifting surfaces. Flight dynamic equations are then introduced to complete the aeroelastic/flight dynamic system equations of motion. Instead of merely considering the flexibility of the wings, the current work allows all members of the vehicle to be flexible. Due to their characteristics of being slender structures, the wings, tail, and fuselage of highly flexible aircraft can be modeled as beams undergoing three dimensional displacements and rotations. New kinematic relationships are developed to handle the split beam systems, such that fully flexible vehicles can be effectively modeled within the existing framework. Different aircraft configurations are modeled and studied, including Single-Wing, Joined-Wing, Blended-Wing-Body, and Flying-Wing configurations. The Lagrange Multiplier Method is applied to model the nodal displacement constraints at the joint locations. Based on the proposed models, roll response and stability studies are conducted on fully flexible and rigidized models. The impacts of the flexibility of different vehicle members on flutter with rigid body motion constraints, flutter in free flight condition, and roll maneuver performance are presented. Also, the static stability of the compressive member of the Joined-Wing configuration is studied. A spatially-distributed discrete gust model is incorporated into the time simulation of the framework. Gust responses of the Flying-Wing configuration subject to stall effects are investigated. A bilinear torsional stiffness model is introduced to study the skin wrinkling due to large bending curvature of the Flying-Wing. The numerical studies illustrate the improvements of the existing reduced-order formulation with new capabilities of both structural modeling and coupled aeroelastic and flight dynamic analysis of fully flexible aircraft.

Fabrication of topical metered dose film forming sprays for pain management.

PubMed

Ranade, Sneha; Bajaj, Amrita; Londhe, Vaishali; Babul, Najib; Kao, Danny

2017-03-30

Topical film-forming metered dose spray formulations were designed for management of pain. Ropivacaine, a local anesthetic is explored for its topical efficacy in alleviating pain. Metered dose spray containers, organic solvents, film forming polymers and permeation enhancers were utilized to fabricate the Metered Dose topical spray. Factors like viscosity, spray pattern, spray angle, volume of actuation, droplet size distribution of the metered dose spray formulation and drying time, flexibility and wash-ability of the film formed after spraying were assessed. Permeation of the drug into the porcine skin was observed based on ex-vivo diffusion studies and confocal microscopy. The results indicated a high level of drug concentration in the skin layers. Anti-nociceptive efficacy of the formulations was assessed on Wistar rats by hot plate and tail flick tests, based on the response to pain perception. The results were comparable to the conventional lidocaine gel. Topical film forming sprays have the ability to provide an accurate, long lasting and patient compliant delivery of drugs on the skin as compared to conventional gels. Copyright © 2017 Elsevier B.V. All rights reserved.

Sonic Boom Propagation Codes Validated by Flight Test

NASA Technical Reports Server (NTRS)

Poling, Hugh W.

1996-01-01

The sonic boom propagation codes reviewed in this study, SHOCKN and ZEPHYRUS, implement current theory on air absorption using different computational concepts. Review of the codes with a realistic atmosphere model confirm the agreement of propagation results reported by others for idealized propagation conditions. ZEPHYRUS offers greater flexibility in propagation conditions and is thus preferred for practical aircraft analysis. The ZEPHYRUS code was used to propagate sonic boom waveforms measured approximately 1000 feet away from an SR-71 aircraft flying at Mach 1.25 to 5000 feet away. These extrapolated signatures were compared to measurements at 5000 feet. Pressure values of the significant shocks (bow, canopy, inlet and tail) in the waveforms are consistent between extrapolation and measurement. Of particular interest is that four (independent) measurements taken under the aircraft centerline converge to the same extrapolated result despite differences in measurement conditions. Agreement between extrapolated and measured signature duration is prevented by measured duration of the 5000 foot signatures either much longer or shorter than would be expected. The duration anomalies may be due to signature probing not sufficiently parallel to the aircraft flight direction.

Torsional and axial compressive properties of tibiotarsal bones of red-tailed hawks (Buteo jamaicensis).

PubMed

Kerrigan, Shannon M; Kapatkin, Amy S; Garcia, Tanya C; Robinson, Duane A; Guzman, David Sanchez-Migallon; Stover, Susan M

2018-04-01

OBJECTIVE To describe the torsional and axial compressive properties of tibiotarsal bones of red-tailed hawks (Buteo jamaicensis). SAMPLE 16 cadaveric tibiotarsal bones from 8 red-tailed hawks. PROCEDURES 1 tibiotarsal bone from each bird was randomly assigned to be tested in torsion, and the contralateral bone was tested in axial compression. Intact bones were monotonically loaded in either torsion (n = 8) or axial compression (8) to failure. Mechanical variables were derived from load-deformation curves. Fracture configurations were described. Effects of sex, limb side, and bone dimensions on mechanical properties were assessed with a mixed-model ANOVA. Correlations between equivalent torsional and compressive properties were determined. RESULTS Limb side and bone dimensions were not associated with any mechanical property. During compression tests, mean ultimate cumulative energy and postyield energy for female bones were significantly greater than those for male bones. All 8 bones developed a spiral diaphyseal fracture and a metaphyseal fissure or fracture during torsional tests. During compression tests, all bones developed a crushed metaphysis and a fissure or comminuted fracture of the diaphysis. Positive correlations were apparent between most yield and ultimate torsional and compressive properties. CONCLUSIONS AND CLINICAL RELEVANCE The torsional and axial compressive properties of tibiotarsal bones described in this study can be used as a reference for investigations into fixation methods for tibiotarsal fractures in red-tailed hawks. Although the comminuted and spiral diaphyseal fractures induced in this study were consistent with those observed in clinical practice, the metaphyseal disruption observed was not and warrants further research.

Flight Tests of a 0.13-Scale Model of the Convair XFY-1 Vertically Rising Airplane with the Lower Vertical Tail Removed, TED No.DE 368

NASA Technical Reports Server (NTRS)

Lovell, Powell M., Jr.

1954-01-01

An experimental investigation has been conducted to determine the dynamic stability and control characteristics in hovering and transition flight of a 0.13-scale flying model of the Convair XFY-1 vertically rising airplane with the lower vertical tail removed. The purpose of the tests was to obtain a general indication of the behavior of a vertically rising airplane of the same general type as the XFY-1 but without a lower vertical tail in order to simplify power-off belly landings in an emergency. The model was flown satisfactorily in hovering flight and in the transition from hovering to normal unstalled forward flight (angle of attack approximately 30deg). From an angle of attack of about 30 down to the lowest angle of attack covered in the flight tests (approximately 15deg) the model became progressively more difficult to control. These control difficulties were attributed partly to a lightly damped Dutch roll oscillation and partly to the fact that the control deflections required for hovering and transition flight were too great for smooth flight at high speeds. In the low-angle-of-attack range not covered in the flight tests, force tests have indicated very low static directional stability which would probably result in poor flight characteristics. It appears, therefore, that the attainment of satisfactory directional stability, at angles of attack less than 10deg, rather than in the hovering and transition ranges of flight is the critical factor in the design of the vertical tail for such a configuration.

Investigation of Aerodynamics Scale Effects for a Generic Fighter Configuration in the National Transonic Facility (Invited)

NASA Technical Reports Server (NTRS)

Tomek, W. G.; Wahls, R. A.; Owens, L. R.; Burner, A. B.; Graves, S. S.; Luckring, J. M.

2003-01-01

Two wind tunnel tests of a generic fighter configuration have been completed in the National Transonic Facility. The primary purpose of the tests was to assess Reynolds number scale effects on a thin-wing, fighter-type configuration up to full-scale flight conditions (that is, Reynolds numbers of the order of 60 million). The tests included longitudinal and lateral/directional studies at subsonic and transonic conditions across a range of Reynolds numbers from that available in conventional wind tunnels to flight conditions. Results are presented for three Mach numbers (0.6, 0.8, and 0.9) and three configurations: 1) Fuselage / Wing, 2) Fuselage / Wing / Centerline Vertical Tail / Horizontal Tail, and 3) Fuselage / Wing / Trailing-Edge Extension / Twin Vertical Tails. Reynolds number effects on the lateral-directional aerodynamic characteristics are presented herein, along with longitudinal data demonstrating the effects of fixing the boundary layer transition location for low Reynolds number conditions. In addition, an improved model videogrammetry system and results are discussed.

Comparative lubrication studies of OH-58A tail rotor drive shaft bearings

NASA Technical Reports Server (NTRS)

Dietrich, M. W.; Parker, R. J.; Zaretsky, E. V.

1972-01-01

Comparative lubrication tests were run with OH-58A helicopter tail rotor drive shaft bearings. The tests were run in an outdoor environment with ambient temperatures ranging from 10 to 75 F. Dust was periodically applied to the bearings to simulate field conditions. The cause of bearing failure was associated with dust penetration. Rotor shaft failure was found to be caused by the shaft rotating in the standard rubber collar due to seizure of the bearings. Bearings with a positive rubbing seal having a MIL-G-81322 grease produced lives greater than with bearings having labyrinth seals and a mineral oil paste lubricant. An elongated collar prevented failure of the rotor shaft during bearing seizure. In a limited test, installation of tail boom shrouds over the bearings which excluded dust and water resulted in bearing lives in excess of 1800 hours or 1200 hours greater than the current 600 hours TBO, regardless of the lubricant-bearing combination used.

A semi-automated vascular access system for preclinical models

NASA Astrophysics Data System (ADS)

Berry-Pusey, B. N.; Chang, Y. C.; Prince, S. W.; Chu, K.; David, J.; Taschereau, R.; Silverman, R. W.; Williams, D.; Ladno, W.; Stout, D.; Tsao, T. C.; Chatziioannou, A.

2013-08-01

Murine models are used extensively in biological and translational research. For many of these studies it is necessary to access the vasculature for the injection of biologically active agents. Among the possible methods for accessing the mouse vasculature, tail vein injections are a routine but critical step for many experimental protocols. To perform successful tail vein injections, a high skill set and experience is required, leaving most scientists ill-suited to perform this task. This can lead to a high variability between injections, which can impact experimental results. To allow more scientists to perform tail vein injections and to decrease the variability between injections, a vascular access system (VAS) that semi-automatically inserts a needle into the tail vein of a mouse was developed. The VAS uses near infrared light, image processing techniques, computer controlled motors, and a pressure feedback system to insert the needle and to validate its proper placement within the vein. The VAS was tested by injecting a commonly used radiolabeled probe (FDG) into the tail veins of five mice. These mice were then imaged using micro-positron emission tomography to measure the percentage of the injected probe remaining in the tail. These studies showed that, on average, the VAS leaves 3.4% of the injected probe in the tail. With these preliminary results, the VAS system demonstrates the potential for improving the accuracy of tail vein injections in mice.

Wind Tunnel Tests Conducted to Develop an Icing Flight Simulator

NASA Technical Reports Server (NTRS)

Ratvasky, Thomas P.

2001-01-01

As part of NASA's Aviation Safety Program goals to reduce aviation accidents due to icing, NASA Glenn Research Center is leading a flight simulator development activity to improve pilot training for the adverse flying characteristics due to icing. Developing flight simulators that incorporate the aerodynamic effects of icing will provide a critical element in pilot training programs by giving pilots a pre-exposure of icing-related hazards, such as ice-contaminated roll upset or tailplane stall. Integrating these effects into training flight simulators will provide an accurate representation of scenarios to develop pilot skills in unusual attitudes and loss-of-control events that may result from airframe icing. In order to achieve a high level of fidelity in the flight simulation, a series of wind tunnel tests have been conducted on a 6.5-percent-scale Twin Otter aircraft model. These wind tunnel tests were conducted at the Wichita State University 7- by 10-ft wind tunnel and Bihrle Applied Research's Large Amplitude Multiple Purpose Facility in Neuburg, Germany. The Twin Otter model was tested without ice (baseline), and with two ice configurations: 1) Ice on the horizontal tail only; 2) Ice on the wing, horizontal tail, and vertical tail. These wind tunnel tests resulted in data bases of aerodynamic forces and moments as functions of angle of attack; sideslip; control surface deflections; forced oscillations in the pitch, roll, and yaw axes; and various rotational speeds. A limited amount of wing and tail surface pressure data were also measured for comparison with data taken at Wichita State and with flight data. The data bases from these tests will be the foundation for a PC-based Icing Flight Simulator to be delivered to Glenn in fiscal year 2001.

Antidepressant-like activity of liposomal formulation containing nimodipine treatment in the tail suspension test, forced swim test and MAOB activity in mice.

PubMed

Moreno, Lina Clara Gayoso E Almendra Ibiapina; Rolim, Hercília Maria Lins; Freitas, Rivelilson Mendes; Santos-Magalhães, Nereide Stela

2016-09-01

Previous studies have shown that intracellular calcium ion dysfunction may be an etiological factor in affective illness. Nimodipine (NMD) is a Ca(2+) channel blocker that has been extensively investigated for therapy of central nervous system (CNS) disorders. In this work, we have evaluated the antidepressant-like activity of nimodipine encapsulated into liposomes (NMD-Lipo) in mice through tail suspension and forced swim assays, as well as MAOB activity. During the tail suspension test, the administration of NMD-Lipo at 0.1, 1 and 10mg/kg was able to promote a reduction in the immobility time of animals greater than the positive control (imipramine). In the forced swim test, the immobility time of mice treated with NMD-Lipo was reduced. This reduction was significantly greater than that found in the animals treated with imipramine and paroxetine. This may suggest that NMD-Lipo provides more antidepressant-like activity than in positive controls. The groups that received a combination of liposomal NMD and antidepressant drugs showed lower immobility time than the groups, which were treated only with imipramine or paroxetine. The mice treated with the combination of NMD-Lipo and reserpine presented an increase in the time of immobility compared with animals treated only with NMD-Lipo. There was a significant decrease in MAOB activity in animals treated with NMD-Lipo compared with untreated animals. The results of the tail suspension test, forced swim test and MAOB activity suggested that the antidepressant activity of NMD-Lipo may be related to an increase in the cerebral monoamine concentrations. Copyright © 2016 Elsevier B.V. All rights reserved.

Tail Service Mast Umbilical Arrival

NASA Image and Video Library

2016-08-02

A crane lifts the first Tail Service Mast Umbilical (TSMU) up for placement on a test stand at the Launch Equipment Test Facility at NASA’s Kennedy Space Center in Florida. Two TSMUs will provide liquid propellants and power to the Space Launch System (SLS) rocket’s core stage engine. Both TSMUs will connect to the zero-level deck on the mobile launcher, providing fuel and electricity to the SLS rocket before it launches on Exploration Mission 1. The TSMU will undergo testing and validation at the LETF to verify it is functioning properly. The center’s Engineering Directorate and the Ground Systems Development and Operations Program are overseeing processing and testing of the umbilicals.

Tail Service Mast Umbilical Arrival

NASA Image and Video Library

2016-08-02

Technician monitors the progress as a crane lowers the first Tail Service Mast Umbilical (TSMU) onto a test stand at the Launch Equipment Test Facility at NASA’s Kennedy Space Center in Florida. Two TSMUs will provide liquid propellants and power to the Space Launch System (SLS) rocket’s core stage engine. Both TSMUs will connect to the zero-level deck on the mobile launcher, providing fuel and electricity to the SLS rocket before it launches on Exploration Mission 1. The TSMU will undergo testing and validation at the LETF to verify it is functioning properly. The center’s Engineering Directorate and the Ground Systems Development and Operations Program are overseeing processing and testing of the umbilicals.

Tail Service Mast Umbilical Arrival

NASA Image and Video Library

2016-08-02

A technician monitors the progress as a crane lifts the first Tail Service Mast Umbilical (TSMU) for transfer to a test stand at the Launch Equipment Test Facility at NASA’s Kennedy Space Center in Florida. Two TSMUs will provide liquid propellants and power to the Space Launch System (SLS) rocket’s core stage engine. Both TSMUs will connect to the zero-level deck on the mobile launcher, providing fuel and electricity to the SLS rocket before it launches on Exploration Mission 1. The TSMU will undergo testing and validation at the LETF to verify it is functioning properly. The center’s Engineering Directorate and the Ground Systems Development and Operations Program are overseeing processing and testing of the umbilicals.

Pleistocene land bridges act as semipermeable agents of avian gene flow in Wallacea.

PubMed

Garg, Kritika M; Chattopadhyay, Balaji; Wilton, Peter R; Malia Prawiradilaga, Dewi; Rheindt, Frank E

2018-08-01

Cyclical periods of global cooling have been important drivers of biotic differentiation throughout the Quaternary. Ice age-induced sea level fluctuations can lead to changing patterns of land connections, both facilitating and disrupting gene flow. In this study, we test if species with differing life histories are differentially affected by Quaternary land connections. We used genome-wide SNPs in combination with mitochondrial gene sequences to analyse levels of divergence and gene flow between two songbird complexes across two Wallacean islands that have been repeatedly connected during glaciations. Although the two bird complexes are similar in ecological attributes, the forest and edge-inhabiting golden whistler Pachycephala pectoralis is comparatively flexible in its diet and niche requirements as compared to the henna-tailed jungle-flycatcher Cyornis colonus, which is largely restricted to the forest interior. Using population-genomic and coalescent approaches, we estimated levels of gene flow, population differentiation and divergence time between the two island populations. We observed higher levels of differentiation, an approximately two to four times deeper divergence time and near-zero levels of gene flow between the two island populations of the more forest-dependent henna-tailed jungle-flycatcher as compared to the more generalist golden whistler. Our results suggest that Quaternary land bridges act as semipermeable agents of gene flow in Wallacea, allowing only certain taxa to connect between islands while others remain isolated. Quaternary land bridges do not accommodate all terrestrial species equally, differing in suitability according to life history and species biology. More generalist species are likely to use Quaternary land connections as a conduit for gene flow between islands whereas island populations of more specialist species may continue to be reproductively isolated even during periods of Quaternary land bridges. Copyright © 2018 Elsevier Inc. All rights reserved.

Structure and mechanical properties of naturally occurring lightweight foam-filled cylinder--the peacock's tail coverts shaft and its components.

PubMed

Liu, Z Q; Jiao, D; Meyers, M A; Zhang, Z F

2015-04-01

Feather shaft, which is primarily featured by a cylinder filled with foam, possesses a unique combination of mechanical robustness and flexibility with a low density through natural evolution and selection. Here the hierarchical structures of peacock's tail coverts shaft and its components are systematically characterized from millimeter to nanometer length scales. The variations in constituent and geometry along the length are examined. The mechanical properties under both dry and wet conditions are investigated. The deformation and failure behaviors and involved strengthening, stiffening and toughening mechanisms are analyzed qualitatively and quantitatively and correlated to the structures. It is revealed that the properties of feather shaft and its components have been optimized through various structural adaptations. Synergetic strengthening and stiffening effects can be achieved in overall rachis owing to increased failure resistance. This study is expected to aid in deeper understandings on the ingenious structure-property design strategies developed by nature, and accordingly, provide useful inspiration for the development of high-performance synthetic foams and foam-filled materials. Copyright © 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Controllable activation of nanoscale dynamics in a disordered protein alters binding kinetics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Callaway, David J. E.; Matsui, Tsutomu; Weiss, Thomas

The phosphorylation of specific residues in a flexible disordered activation loop yields precise control of signal transduction. One paradigm is the phosphorylation of S339/S340 in the intrinsically disordered tail of the multi-domain scaffolding protein NHERF1, which affects the intracellular localization and trafficking of NHERF1 assembled signaling complexes. Using neutron spin echo spectroscopy (NSE), we show salt-concentration-dependent excitation of nanoscale motion at the tip of the C-terminal tail in the phosphomimic S339D/S340D mutant. The “tip of the whip” that is unleashed is near the S339/S340 phosphorylation site and flanks the hydrophobic Ezrin-binding motif. The kinetic association rate constant of the bindingmore » of the S339D/S340D mutant to the FERM domain of Ezrin is sensitive to buffer salt concentration, correlating with the excited nanoscale dynamics. The results suggest that electrostatics modulates the activation of nanoscale dynamics of an intrinsically disordered protein, controlling the binding kinetics of signaling partners. Furthermore NSE can pinpoint the nanoscale dynamics changes in a highly specific manner.« less

Structure of a headful DNA-packaging bacterial virus at 2.9 Å resolution by electron cryo-microscopy

PubMed Central

Zhao, Haiyan; Li, Kunpeng; Lynn, Anna Y.; Aron, Keith E.; Yu, Guimei; Jiang, Wen; Tang, Liang

2017-01-01

The enormous prevalence of tailed DNA bacteriophages on this planet is enabled by highly efficient self-assembly of hundreds of protein subunits into highly stable capsids. These capsids can stand with an internal pressure as high as ∼50 atmospheres as a result of the phage DNA-packaging process. Here we report the complete atomic model of the headful DNA-packaging bacteriophage Sf6 at 2.9 Å resolution determined by electron cryo-microscopy. The structure reveals the DNA-inflated, tensed state of a robust protein shell assembled via noncovalent interactions. Remarkable global conformational polymorphism of capsid proteins, a network formed by extended N arms, mortise-and-tenon–like intercapsomer joints, and abundant β-sheet–like mainchain:mainchain intermolecular interactions, confers significant strength yet also flexibility required for capsid assembly and DNA packaging. Differential formations of the hexon and penton are mediated by a drastic α–helix-to-β–strand structural transition. The assembly scheme revealed here may be common among tailed DNA phages and herpesviruses. PMID:28320961

Controllable activation of nanoscale dynamics in a disordered protein alters binding kinetics

DOE PAGES

Callaway, David J. E.; Matsui, Tsutomu; Weiss, Thomas; ...

2017-03-08

The phosphorylation of specific residues in a flexible disordered activation loop yields precise control of signal transduction. One paradigm is the phosphorylation of S339/S340 in the intrinsically disordered tail of the multi-domain scaffolding protein NHERF1, which affects the intracellular localization and trafficking of NHERF1 assembled signaling complexes. Using neutron spin echo spectroscopy (NSE), we show salt-concentration-dependent excitation of nanoscale motion at the tip of the C-terminal tail in the phosphomimic S339D/S340D mutant. The “tip of the whip” that is unleashed is near the S339/S340 phosphorylation site and flanks the hydrophobic Ezrin-binding motif. The kinetic association rate constant of the bindingmore » of the S339D/S340D mutant to the FERM domain of Ezrin is sensitive to buffer salt concentration, correlating with the excited nanoscale dynamics. The results suggest that electrostatics modulates the activation of nanoscale dynamics of an intrinsically disordered protein, controlling the binding kinetics of signaling partners. Furthermore NSE can pinpoint the nanoscale dynamics changes in a highly specific manner.« less

Elemental mobility in sulfidic mine tailings reclaimed with paper mill by-products as sealing materials.

PubMed

Jia, Yu; Stahre, Nanna; Mäkitalo, Maria; Maurice, Christian; Öhlander, Björn

2017-09-01

Sealing layers made of two alkaline paper mill by-products, fly ash and green liquor dregs, were placed on top of 50-year-old sulfide-containing tailings as a full-scale remediation approach. The performance and effectiveness of the sealing layers with high water content for an oxygen barrier and low hydraulic conductivity for a sealing layer in preventing the formation of acid rock drainage were evaluated 5 years after the remediation. The leaching behavior of the covered tailings was studied using batch leaching tests (L/S ratio 10 L/kg). The leaching results revealed that, in general, the dregs- and ash-covered tailings released relatively lower concentrations of many elements contained in acid rock drainage compared to those from the uncovered tailings. A change in the chemical composition and mineralogical state of the tailings was observed for the tailings beneath the covers. The increase in pH caused by the alkaline materials promoted metal precipitation. Geochemical modeling using PHREEQC confirmed most of the geochemical changes of the covered tailings. Both the ash and dregs showed potential to function as sealing materials in terms of their geochemical properties. However, mobilization of Zn and Ni from the lower part of the dregs-covered tailings was observed. The same phenomenon was observed for the lower part of the ash-covered tailings. Ash showed advantages over dregs as a cover material; based on geochemical studies, the ash immobilized more elements than the dregs did. Lysimeters were installed below the sealing layers, and infiltrating water chemistry and hydrology were studied to monitor the amount and quality of the leachate percolating through.

Response of key soil parameters during compost-assisted phytostabilization in extremely acidic tailings: effect of plant species.

PubMed

Solís-Dominguez, Fernando A; White, Scott A; Hutter, Travis Borrillo; Amistadi, Mary Kay; Root, Robert A; Chorover, Jon; Maier, Raina M

2012-01-17

Phytostabilization of mine tailings acts to mitigate both eolian dispersion and water erosion events which can disseminate barren tailings over large distances. This technology uses plants to establish a vegetative cover to permanently immobilize contaminants in the rooting zone, often requiring addition of an amendment to assist plant growth. Here we report the results of a greenhouse study that evaluated the ability of six native plant species to grow in extremely acidic (pH ∼ 2.5) metalliferous (As, Pb, Zn: 2000-3000 mg kg(-1)) mine tailings from Iron King Mine Humboldt Smelter Superfund site when amended with a range of compost concentrations. Results revealed that three of the six plant species tested (buffalo grass, mesquite, and catclaw acacia) are good candidates for phytostabilization at an optimum level of 15% compost (w/w) amendment showing good growth and minimal shoot accumulation of metal(loid)s. A fourth candidate, quailbush, also met all criteria except for exceeding the domestic animal toxicity limit for shoot accumulation of zinc. A key finding of this study was that the plant species that grew most successfully on these tailings significantly influenced key tailings parameters; direct correlations between plant biomass and both increased tailings pH and neutrophilic heterotrophic bacterial counts were observed. We also observed decreased iron oxidizer counts and decreased bioavailability of metal(loid)s mainly as a result of compost amendment. Taken together, these results suggest that the phytostabilization process reduced tailings toxicity as well as the potential for metal(loid) mobilization. This study provides practical information on plant and tailings characteristics that is critically needed for successful implementation of assisted phytostabilization on acidic, metalliferous mine tailings sites.

Response of Key Soil Parameters During Compost-Assisted Phytostabilization in Extremely Acidic Tailings: Effect of Plant Species

PubMed Central

Solís-Dominguez, Fernando A.; White, Scott A.; Hutter, Travis Borrillo; Amistadi, Mary Kay; Root, Robert A.; Chorover, Jon; Maier, Raina M.

2012-01-01

Phytostabilization of mine tailings acts to mitigate both eolian dispersion and water erosion events which can disseminate barren tailings over large distances. This technology uses plants to establish a vegetative cover to permanently immobilize contaminants in the rooting zone, often requiring addition of an amendment to assist plant growth. Here we report the results of a greenhouse study that evaluated the ability of six native plant species to grow in extremely acidic (pH ~ 2.5) metalliferous (As, Pb, Zn: 2000–3000 mg kg−1) mine tailings from Iron King Mine Humboldt Smelter Superfund site when amended with a range of compost concentrations. Results revealed that three of the six plant species tested (buffalo grass, mesquite, and catclaw acacia) are good candidates for phytostabilization at an optimum level of 15% compost (w/w) amendment showing good growth and minimal shoot accumulation of metal(loid)s. A fourth candidate, quailbush, also met all criteria except for exceeding the domestic animal toxicity limit for shoot accumulation of zinc. A key finding of this study was that the plant species that grew most successfully on these tailings significantly influenced key tailings parameters; direct correlations between plant biomass and both increased tailings pH and neutrophilic heterotrophic bacterial counts were observed. We also observed decreased iron oxidizer counts and decreased bioavailability of metal(loid)s mainly as a result of compost amendment. Taken together, these results suggest that the phytostabilization process reduced tailings toxicity as well as the potential for metal(loid) mobilization. This study provides practical information on plant and tailings characteristics that is critically needed for successful implementation of assisted phytostabilization on acidic, metalliferous mine tailings sites. PMID:22191663

10 CFR Appendix A to Part 40 - Criteria Relating to the Operation of Uranium Mills and the Disposition of Tailings or Wastes...

Code of Federal Regulations, 2011 CFR

2011-01-01

... to be relied upon for seepage control, tests must be conducted with representative tailings solutions... licensee shall control, minimize, or eliminate post-closure escape of nonradiological hazardous... beyond the control of the licensee. The phrase permits consideration of the cost of compliance only to...

10 CFR Appendix A to Part 40 - Criteria Relating to the Operation of Uranium Mills and the Disposition of Tailings or Wastes...

Code of Federal Regulations, 2010 CFR

2010-01-01

... to be relied upon for seepage control, tests must be conducted with representative tailings solutions... itself. (6) The design requirements in this criterion for longevity and control of radon releases apply... licensee shall control, minimize, or eliminate post-closure escape of nonradiological hazardous...

Geochemical and mineralogical characterization of a neutral, low-sulfide/high-carbonate tailings impoundment, Markušovce, eastern Slovakia.

PubMed

Hiller, Edgar; Petrák, Marián; Tóth, Roman; Lalinská-Voleková, Bronislava; Jurkovič, L'ubomír; Kučerová, Gabriela; Radková, Anežka; Sottník, Peter; Vozár, Jaroslav

2013-11-01

Tailings deposits generated from mining activities represent a potential risk for the aquatic environment through the release of potentially toxic metals and metalloids occurring in a variety of minerals present in the tailings. Physicochemical and mineralogical characteristics of tailings such as total concentrations of chemical elements, pH, ratio of acid-producing to acid-neutralizing minerals, and primary and secondary mineral phases are very important factors that control the actual release of potentially toxic metals and metalloids from the tailings to the environment. The aims of this study are the determination of geochemical and mineralogical characteristics of tailings deposited in voluminous impoundment situated near the village of Markušovce (eastern Slovakia) and identification of the processes controlling the mobility of selected toxic metals (Cu, Hg) and metalloids (As, Sb). The studied tailings have unique features in comparison with the other tailings investigated previously because of the specific mineral assemblage primarily consisting of barite, siderite, quartz, and minor sulfides. To meet the aims, samples of the tailings were collected from 3 boreholes and 15 excavated pits and subjected to bulk geochemical analyses (i.e., determination of chemical composition, pH, Eh, acid generation, and neutralization potentials) combined with detailed mineralogical characterization using optical microscopy, X-ray diffraction (XRD), electron microprobe analysis (EMPA), and micro-X-ray diffraction (μ-XRD). Additionally, the geochemical and mineralogical factors controlling the transfer of potentially toxic elements from tailings to waters were also determined using short-term batch test (European norm EN 12457), sampling of drainage waters and speciation-equilibrium calculations performed with PHREEQC. The tailings mineral assemblage consists of siderite, barite, quartz, and dolomite. Sulfide minerals constitute only a minor proportion of the tailings mineral assemblage and their occurrence follows the order: chalcopyrite > pyrite > tetrahedrite>arsenopyrite. The mineralogical composition of the tailings corresponds well to the primary mineralization mined. The neutralization capacity of the tailings is high, as confirmed by the values of neutralization potential to acid generation potential ratio, ranging from 6.7 to 63.9, and neutral to slightly alkaline pH of the tailings (paste pH 7.16-8.12) and the waters (pH 7.00-8.52). This is explained by abundant occurrence of carbonate minerals in the tailings, which readily neutralize the acidity generated by sulfide oxidation. The total solid-phase concentrations of metal(loid)s decrease as Cu>Sb>Hg>As and reflect the proportions of sulfides present in the tailings. Sulfide oxidation generally extends to a depth of 2 m. μ-XRD and EMPA were used to study secondary products developed on the surface of sulfide minerals and within the tailings. The main secondary minerals identified are goethite and X-ray amorphous Fe oxyhydroxides and their occurrence decreases with increasing tailings depth. Secondary Fe phases are found as mineral coatings or individual grains and retain relatively high amounts of metal(loid)s (up to 57.6 wt% Cu, 1.60 wt% Hg, 23.8 wt% As, and 2.37 wt% Sb). Based on batch leaching tests and lysimeter results, the mobility of potentially toxic elements in the tailings is low. The limited mobility of metals and metalloids is due to their retention by Fe oxyhydroxides and low solubilities of metal(loid)-bearing sulfides. The observations are consistent with PHREEQC calculations, which predict the precipitation of Fe oxyhydroxides as the main solubility-controlling mineral phases for As, Cu, Hg, and Sb. Waters discharging from tailings impoundment are characterized by a neutral to slightly alkaline pH (7.52-7.96) and low concentrations of dissolved metal(loid)s (<5-7.0 μg/L Cu, <0.1-0.3 μg/L Hg, 5.0-16 μg/L As, and 5.0-43 μg/L Sb). Primary factors influencing aqueous chemistry at the site are mutual processes of sulfide oxidation and carbonate dissolution as well as precipitation reactions and sorption onto hydrous ferric oxides abundantly present at the discharge of the impoundment waters. The results of the study show that, presently, there are no threats of acid mine drainage formation at the site and significant contamination of natural aquatic ecosystem in the close vicinity of the tailings impoundment.

Effect of nozzle and vertical-tail variables on the performance of a 3-surface F-15 model at transonic Mach numbers. [Langley 16 foot transonic tunnel

NASA Technical Reports Server (NTRS)

Pendergraft, O. C., Jr.; Bare, E. A.

1982-01-01

An investigation was conducted in the Langley 16 foot transonic tunnel to determine the longitudinal aerodynamic characteristics of twin two dimensional nozzles and twin baseline axisymmetric nozzles installed on a fully metric 0.047 scale model of the F-15 three surface configuration (canards, wing, horizontal tails). The effects on performance of two dimensional nozzle in flight thrust reversing, locations and orientation of the vertical tails, and deflections of the horizontal tails were also determined. Test data were obtained at static conditions and at Mach numbers from 0.60 to 1.20 over an angle of attack range from -2 deg to 15 deg. Nozzle pressure ratio was varied from jet off to about 6.5.

Impacts of Space Shuttle thermal protection system tile on F-15 aircraft vertical tile

NASA Technical Reports Server (NTRS)

Ko, W. L.

1985-01-01

Impacts of the space shuttle thermal protection system (TPS) tile on the leading edge and the side of the vertical tail of the F-15 aircraft were analyzed under different TPS tile orientations. The TPS tile-breaking tests were conducted to simulate the TPS tile impacts. It was found that the predicted tile impact forces compare fairly well with the tile-breaking forces, and the impact forces exerted on the F-15 aircraft vertical tail were relatively low because a very small fraction of the tile kinetic energy was dissipated in the impact, penetration, and fracture of the tile. It was also found that the oblique impact of the tile on the side of the F-15 aircraft vertical tail was unlikely to dent the tail surface.

On the propulsive efficiency of rotating elastica.

NASA Astrophysics Data System (ADS)

Fermigier, Marc; Champagne, Nicolas; Laik, Eric; Marthelot, Joel; Du Roure, Olivia

2007-11-01

A majority of microorganisms propel themselves with long flexible cilia or flagella. Understanding in detail the hydrodynamics of such propulsion mechanisms is important both from biological and engineering point of views, in particular to design artificial microswimmers. We report an experimental investigation of the propulsive force delivered by a rotating elastic filament. Macroscopic filaments made of an elastomer (Young's modulus E) loaded with solid particles to match the density of the suspending liquid are rotated at constant velocity φ in a bath of glycerin. Their three dimensional shape is time independent but varies with φ and aspect ratio L/a. The force on the filament is computed from the experimental shape using a slender body approximation (ratio of perpendicular and parallel friction coefficients : ζ= 2 ζ). The evolution of axial force is captured by a single dimensionless parameter comparing viscous and elastic stresses: Sp = (ηφ/E) (L/a)^4. As for a planar oscillating flexible tail a maximum force is found at Sp 1.

Flight-Test Evaluation of the Longitudinal Stability and Control Characteristics of 0.5-Scale Models of the Fairchild Lark Pilotless-Aircraft Configuration: Standard Configuration with Wing Flaps Deflected 60 Degrees and Model having Tail in Line with Wings, TED No. NACA 2387

NASA Technical Reports Server (NTRS)

Stone, David G.

1947-01-01

Flight tests were conducted at the Flight Test Station of the Pilotless Aircraft Research Division at Wallop Island, Va., to determine the longitudinal control and stability characteristics of 0.5-scale models of the Fairchild Lark pilotless aircraft with the tail in line with the wings a d with the horizontal wing flaps deflected 60 deg. The data were obtained by the use of a telemeter and by radar tracking.

Bell-Curve Genetic Algorithm for Mixed Continuous and Discrete Optimization Problems

NASA Technical Reports Server (NTRS)

Kincaid, Rex K.; Griffith, Michelle; Sykes, Ruth; Sobieszczanski-Sobieski, Jaroslaw

2002-01-01

In this manuscript we have examined an extension of BCB that encompasses a mix of continuous and quasi-discrete, as well as truly-discrete applications. FVe began by testing two refinements to the discrete version of BCB. The testing of midpoint versus fitness (Tables 1 and 2) proved inconclusive. The testing of discrete normal tails versus standard mutation showed was conclusive and demonstrated that the discrete normal tails are better. Next, we implemented these refinements in a combined continuous and discrete BCB and compared the performance of two discrete distance on the hub problem. Here we found when "order does matter" it pays to take it into account.

Free-Suspension Residual Flexibility Testing of Space Station Pathfinder: Comparison to Fixed-Base Results

NASA Technical Reports Server (NTRS)

Tinker, Michael L.

1998-01-01

Application of the free-suspension residual flexibility modal test method to the International Space Station Pathfinder structure is described. The Pathfinder, a large structure of the general size and weight of Space Station module elements, was also tested in a large fixed-base fixture to simulate Shuttle Orbiter payload constraints. After correlation of the Pathfinder finite element model to residual flexibility test data, the model was coupled to a fixture model, and constrained modes and frequencies were compared to fixed-base test. modes. The residual flexibility model compared very favorably to results of the fixed-base test. This is the first known direct comparison of free-suspension residual flexibility and fixed-base test results for a large structure. The model correlation approach used by the author for residual flexibility data is presented. Frequency response functions (FRF) for the regions of the structure that interface with the environment (a test fixture or another structure) are shown to be the primary tools for model correlation that distinguish or characterize the residual flexibility approach. A number of critical issues related to use of the structure interface FRF for correlating the model are then identified and discussed, including (1) the requirement of prominent stiffness lines, (2) overcoming problems with measurement noise which makes the antiresonances or minima in the functions difficult to identify, and (3) the use of interface stiffness and lumped mass perturbations to bring the analytical responses into agreement with test data. It is shown that good comparison of analytical-to-experimental FRF is the key to obtaining good agreement of the residual flexibility values.

Bioprocessing-Based Approach for Bitumen/Water/Fines Separation and Hydrocarbon Recovery from Oil Sands Tailings

DOE PAGES

Brigmon, Robin L.; Berry, Christopher J.; Wade, Arielle; ...

2016-05-04

Oil sands are a major source of oil, but their industrial processing generates tailings ponds that are an environmental hazard. The main concerns are mature fine tailings (MFT) composed of residual hydrocarbons, water, and fine clay. Tailings ponds include toxic contaminants such as heavy metals, and toxic organics including naphthenics. Naphthenic acids and polyaromatic hydrocarbons (PAHs) degrade very slowly and pose a long-term threat to surface and groundwater, as they can be transported in the MFT. Research into improved technologies that would enable densification and settling of the suspended particles is ongoing. In batch tests, BioTiger™, a microbial consortium thatmore » can metabolize PAHs, demonstrated improved oil sands tailings settling from a Canadian tailings pond. Results also showed, depending on the timing of the measurements, lower suspended solids and turbidity. Elevated total organic carbon was observed in the first 48 hours in the BioTiger™-treated columns and then decreased in overlying water. Oil sands tailings mixed with BioTiger™ showed a two-fold reduction in suspended solids within 24 hours as compared to abiotic controls. The tailings treated with BioTiger™ increased in microbial densities three orders of magnitude from 8.5 × 105 CFU/mL to 1.2 × 108 CFU/mL without any other carbon or energy source added, indicating metabolism of hydrocarbons and other available nutrients. Results demonstrated that bioaugmentation of BioTiger™ increased separation of organic carbon from particles in oil sands and enhanced settling with tailings with improved water quality.« less

Crystal Structure of an Activated Variant of Small Heat Shock Protein Hsp16.5

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mchaourab, Hassane S.; Lin, Yi-Lun; Spiller, Benjamin W.

How does the sequence of a single small heat shock protein (sHSP) assemble into oligomers of different sizes? To gain insight into the underlying structural mechanism, we determined the crystal structure of an engineered variant of Methanocaldococcus jannaschii Hsp16.5 wherein a 14 amino acid peptide from human heat shock protein 27 (Hsp27) was inserted at the junction of the N-terminal region and the {alpha}-crystallin domain. In response to this insertion, the oligomer shell expands from 24 to 48 subunits while maintaining octahedral symmetry. Oligomer rearrangement does not alter the fold of the conserved {alpha}-crystallin domain nor does it disturb themore » interface holding the dimeric building block together. Rather, the flexible C-terminal tail of Hsp16.5 changes its orientation relative to the {alpha}-crystallin domain which enables alternative packing of dimers. This change in orientation preserves a peptide-in-groove interaction of the C-terminal tail with an adjacent {beta}-sandwich, thereby holding the assembly together. The interior of the expanded oligomer, where substrates presumably bind, retains its predominantly nonpolar character relative to the outside surface. New large windows in the outer shell provide increased access to these substrate-binding regions, thus accounting for the higher affinity of this variant to substrates. Oligomer polydispersity regulates sHSPs chaperone activity in vitro and has been implicated in their physiological roles. The structural mechanism of Hsp16.5 oligomer flexibility revealed here, which is likely to be highly conserved across the sHSP superfamily, explains the relationship between oligomer expansion observed in disease-linked mutants and changes in chaperone activity.« less

Crystal structure of an activated variant of small heat shock protein Hsp16.5.

PubMed

McHaourab, Hassane S; Lin, Yi-Lun; Spiller, Benjamin W

2012-06-26

How does the sequence of a single small heat shock protein (sHSP) assemble into oligomers of different sizes? To gain insight into the underlying structural mechanism, we determined the crystal structure of an engineered variant of Methanocaldococcus jannaschii Hsp16.5 wherein a 14 amino acid peptide from human heat shock protein 27 (Hsp27) was inserted at the junction of the N-terminal region and the α-crystallin domain. In response to this insertion, the oligomer shell expands from 24 to 48 subunits while maintaining octahedral symmetry. Oligomer rearrangement does not alter the fold of the conserved α-crystallin domain nor does it disturb the interface holding the dimeric building block together. Rather, the flexible C-terminal tail of Hsp16.5 changes its orientation relative to the α-crystallin domain which enables alternative packing of dimers. This change in orientation preserves a peptide-in-groove interaction of the C-terminal tail with an adjacent β-sandwich, thereby holding the assembly together. The interior of the expanded oligomer, where substrates presumably bind, retains its predominantly nonpolar character relative to the outside surface. New large windows in the outer shell provide increased access to these substrate-binding regions, thus accounting for the higher affinity of this variant to substrates. Oligomer polydispersity regulates sHSPs chaperone activity in vitro and has been implicated in their physiological roles. The structural mechanism of Hsp16.5 oligomer flexibility revealed here, which is likely to be highly conserved across the sHSP superfamily, explains the relationship between oligomer expansion observed in disease-linked mutants and changes in chaperone activity.

Seroprevalence of Toxoplasma gondii, Sarcocystis neurona, and Encephalitozoon cuniculi in three species of lemurs from St. Catherines Island, GA, USA.

PubMed

Yabsley, Michael J; Jordan, Carly N; Mitchell, Sheila M; Norton, Terry M; Lindsay, David S

2007-03-15

In the current study, we determined the seroprevalence of Toxoplasma gondii, Sarcocystis neurona, and Encephalitozoon cuniculi in three species of lemurs from St. Catherines Island, Georgia. Serum samples were tested from 52 ring-tailed lemurs (Lemur catta), six blue-eyed black lemurs (Eulemur macaco flavifrons), and four black and white ruffed lemurs (Varecia variegata variegata) using an agglutination assay. Three ring-tailed lemurs (5.8%) were positive for T. gondii (titer of 1:50); one ring-tailed lemur (1.9%) and one black and white ruffed lemur (25%) were positive for S. neurona (titers of 1:1000); and one ring-tailed lemur (1.9%) was positive for E. cuniculi (titer of 1:400). All blue-eyed black lemurs were negative for antibodies to T. gondii, S. neurona, and E. cuniculi. This is the first detection of antibodies to T. gondii in ring-tailed lemurs and antibodies to S. neurona and E. cuniculi in any species of prosimian.

Tail loss compromises immunity in the many-lined skink, Eutropis multifasciata

NASA Astrophysics Data System (ADS)

Kuo, Chi-Chien; Yao, Chiou-Ju; Lin, Te-En; Liu, Hsu-Che; Hsu, Yu-Cheng; Hsieh, Ming-Kun; Huang, Wen-San

2013-04-01

Tail autotomy incurs energetic costs, and thus, a trade-off in resource allocation may lead to compromised immunity in lizards. We tested the hypothesis that tailless lizards will favor constitutive innate immunity responses over an energetically costly inflammatory response. The influence of fasting and colorful ornamentation was also investigated. We experimentally induced tail autotomy in the lizard Eutropis multifasciata and found that inflammation was suppressed by tail loss, but not further affected by fasting; the suppressive effect of colorful ornamentation was manifested only in males, but not in females. Constitutive innate immunity was not affected by any of these factors. As expected, only costly inflammation was compromised, and a less expensive constitutive innate immunity might be favored as a competent first-line defense during energetically demanding periods. After considering conventional trade-offs among tail regeneration and reproduction, further extending these studies to incorporate disease risk and how this influences escape responses to predators and future reproduction would make worthwhile studies.

Column Testing and 1D Reactive Transport Modeling to Evaluate Uranium Plume Persistence Processes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Johnson, Raymond H.; Morrison, Stan; Morris, Sarah

Motivation for Study: Natural flushing of contaminants at various U.S. Department of Energy Office of Legacy Management sites is not proceeding as quickly as predicted (plume persistence) Objectives: Help determine natural flushing rates using column tests. Use 1D reactive transport modeling to better understand the major processes that are creating plume persistence Approach: Core samples from under a former mill tailings area Tailings have been removed. Column leaching using lab-prepared water similar to nearby Gunnison River water. 1D reactive transport modeling to evaluate processes

Determination of the Stability and Control Characteristics of a Tailless All-Wing Airplane Model with Sweepback in the Langley Free-Flight Tunnel

NASA Technical Reports Server (NTRS)

Seacord, Charles L.; Campbell, John P.

1945-01-01

Force and flight tests were performance on an all-wing model with windmilling propellers. Tests were conducted with deflected and retracted flaps, with and without auxiliary vertical tail surfaces, and with different centers of gravity and trim coefficients. Results indicate serious reduction of stick-fixed longitudinal stability because of wing-tip stalling at high lift coefficient. Directional stability without vertical tail is undesirably low. Low effective dihedral should be maintained. Elevator and rudder control system is satisfactory.

PAPP-A affects tendon structure and mechanical properties.

PubMed

Yang, Tai-Hua; Thoreson, Andrew R; An, Kai-Nan; Zhao, Chunfeng; Conover, Cheryl A; Amadio, Peter C

2015-10-01

Pregnancy-associated plasma protein-A (PAPP-A) serves to increase local insulin-like growth factor (IGF) stimulation of proliferation and differentiation in many tissues through proteolysis of inhibitory IGF-binding proteins. The purpose of this study was to investigate the effects of PAPP-A on tendon structure and mechanical properties. A total of 30 tails from 6-month-old mice were tested with 10 tails in each of following groups: PAPP-A knockout (KO), skeletal-specific PAPP-A overexpressing transgenic (Tg) and wild type (WT). Morphologically, the total tail cross-sectional area (CSA), individual tissue CSAs of bone, muscle and tendon, and fascicle diameter were measured. A fascicle pullout test was performed to assess stiffness and strength of interfascicular structures. Fascicles were mechanically characterized through low and high displacement rate uniaxial tension tests providing modulus at each rate, hysteresis area and stress relaxation ratio. The KO mice had a smaller total tail CSA (p<0.05), fascicle diameter (p<0.05), absolute tendon CSA (p<0.05), fast and slow stiffness (p<0.05 for both) and larger hysteresis area (p<0.05) compared to WT and Tg mice. On the other hand, the Tg mice had a larger fascicle diameter (p<0.05), absolute tendon CSA (p<0.05), higher interfascicular strength and stiffness (p<0.05) and lower fascicular modulus at low displacement rates (p<0.05) compared to WT and KO mice. Tg mice also had larger total tail CSA area (p<0.05) and smaller hysteresis area (p<0.05) than KO mice, and larger normalized tendon CSA (p<0.05) than WT mice. Based on these data, we conclude that PAPP-A affects fascicle structure, thereby affecting tendon phenotype. Copyright © 2015 Elsevier Inc. All rights reserved.

Flurbiprofen in rapid eye movement sleep deprivation induced hyperalgesia.

PubMed

Gürel, Elif Ezgi; Ural, Keremcan; Öztürk, Gülnur; Öztürk, Levent

2014-04-10

Rapid eye movement (REM) sleep deprivation induces hyperalgesia in healthy rats. Here, we evaluated the effects of flurbiprofen, an anti-inflammatory and neuroprotective agent, on the increased thermal responses observed in REM sleep deprived rats. Forty female rats were divided into four groups following 96-hour REM sleep deprivation: intraperitoneal injections of placebo, and flurbiprofen 5 mg/kg, 15 mg/kg and 40 mg/kg were made in CONT (n=10), FBP5, FBP15 and FBP40 groups respectively. Pain threshold measurements were performed three times at baseline (0.hour), at the end of REM sleep deprivation (96.hour) and at 1 h after injections (97.hour) by hot plate and tail-flick tests. REM sleep deprivation induced a significant decrease in pain thresholds of all rats (hotplate: 0.hour vs 96.hour, 9.75±2.85 vs 5.10±2.02, p<0.001; tail flick: 0.hour vs 96.hour, 11.92±4.62 vs 7.92±5.15, p<0.001). Flurbiprofen in 15 mg/kg and 40 mg/kg doses significantly improved pain tolerance measured by tail flick test (tail flick in FBP15 and FBP40 groups: 96.hour vs 97.hour, 7.01±4.97 vs 8.34±3.61 and 5.06±1.57 vs 7.04±2.49, p<0.05 for both). 96 h of REM sleep deprivation resulted in reduced pain thresholds in both hot plate and tail flick tests. Flurbiprofen was used for the first time in a rat model of REM sleep deprivation, and it provided anti-nociceptive effects in 15 mg/kg and 40 mg/kg doses. Flurbiprofen may have the potential for treatment of painful syndromes accompanying insomnia or sleep loss. Copyright © 2014 Elsevier Inc. All rights reserved.

A New Test of Attention in Listening (TAIL) Predicts Auditory Performance

PubMed Central

Zhang, Yu-Xuan; Barry, Johanna G.; Moore, David R.; Amitay, Sygal

2012-01-01

Attention modulates auditory perception, but there are currently no simple tests that specifically quantify this modulation. To fill the gap, we developed a new, easy-to-use test of attention in listening (TAIL) based on reaction time. On each trial, two clearly audible tones were presented sequentially, either at the same or different ears. The frequency of the tones was also either the same or different (by at least two critical bands). When the task required same/different frequency judgments, presentation at the same ear significantly speeded responses and reduced errors. A same/different ear (location) judgment was likewise facilitated by keeping tone frequency constant. Perception was thus influenced by involuntary orienting of attention along the task-irrelevant dimension. When information in the two stimulus dimensions were congruent (same-frequency same-ear, or different-frequency different-ear), response was faster and more accurate than when they were incongruent (same-frequency different-ear, or different-frequency same-ear), suggesting the involvement of executive control to resolve conflicts. In total, the TAIL yielded five independent outcome measures: (1) baseline reaction time, indicating information processing efficiency, (2) involuntary orienting of attention to frequency and (3) location, and (4) conflict resolution for frequency and (5) location. Processing efficiency and conflict resolution accounted for up to 45% of individual variances in the low- and high-threshold variants of three psychoacoustic tasks assessing temporal and spectral processing. Involuntary orientation of attention to the irrelevant dimension did not correlate with perceptual performance on these tasks. Given that TAIL measures are unlikely to be limited by perceptual sensitivity, we suggest that the correlations reflect modulation of perceptual performance by attention. The TAIL thus has the power to identify and separate contributions of different components of attention to auditory perception. PMID:23300934

Airplane numerical simulation for the rapid prototyping process

NASA Astrophysics Data System (ADS)

Roysdon, Paul F.

Airplane Numerical Simulation for the Rapid Prototyping Process is a comprehensive research investigation into the most up-to-date methods for airplane development and design. Uses of modern engineering software tools, like MatLab and Excel, are presented with examples of batch and optimization algorithms which combine the computing power of MatLab with robust aerodynamic tools like XFOIL and AVL. The resulting data is demonstrated in the development and use of a full non-linear six-degrees-of-freedom simulator. The applications for this numerical tool-box vary from un-manned aerial vehicles to first-order analysis of manned aircraft. A Blended-Wing-Body airplane is used for the analysis to demonstrate the flexibility of the code from classic wing-and-tail configurations to less common configurations like the blended-wing-body. This configuration has been shown to have superior aerodynamic performance -- in contrast to their classic wing-and-tube fuselage counterparts -- and have reduced sensitivity to aerodynamic flutter as well as potential for increased engine noise abatement. Of course without a classic tail elevator to damp the nose up pitching moment, and the vertical tail rudder to damp the yaw and possible rolling aerodynamics, the challenges in lateral roll and yaw stability, as well as pitching moment are not insignificant. This thesis work applies the tools necessary to perform the airplane development and optimization on a rapid basis, demonstrating the strength of this tool through examples and comparison of the results to similar airplane performance characteristics published in literature.

Statical longitudinal stability of airplanes

NASA Technical Reports Server (NTRS)

Warner, Edward P

1921-01-01

This report, which is a continuation of the "Preliminary report on free flight testing" (report no. NACA-TR-70), presents a detailed theoretical analysis of statical stability with free and locked controls and also the results of many free flight test on several types of airplanes. In developing the theory of stability with locked controls an expression for pitching moment is derived in simple terms by considering the total moment as the sum of the moments due to wings and tail surface. This expression, when differentiated with respect to angle of incidence, enables an analysis to be made of the factors contributing to the pitching moment. The effects of slipstream and down wash are also considered and it is concluded that the C. G. Location has but slight effect or stability, and that stability is much improved by increasing the efficiency of the tail surfaces, which may be done by using an "inverted" tail plane. The results of free flight tests with locked controls are discussed at length and it is shown that the agreement between the experimental results and theory is very satisfactory. The theory of stability with free controls is not amendable to the simple mathematical treatment used in the case of locked controls, but a clear statement of the conditions enables several conclusions to be drawn, one of which is that the fixed tail surfaces should be much larger than the movable surfaces.

The flotation tailings of the former Pb-Zn mine of Touiref (NW Tunisia): mineralogy, mine drainage prediction, base-metal speciation assessment and geochemical modeling.

PubMed

Othmani, Mohamed Ali; Souissi, Fouad; Bouzahzah, Hassan; Bussière, Bruno; da Silva, Eduardo Ferreira; Benzaazoua, Mostafa

2015-02-01

The underground extraction of Pb-Zn mineralization in the Touiref area stopped in 1958. A large volume of flotation tailings (more than 500 Mt) containing sulfides were deposited in a tailings impoundment. The goals of this study are to evaluate the neutralization capacity of the unoxidized and oxidized tailings, to assess the speciation of metals between the different components of the tailings material, and to assess the mobility of metals and the secondary minerals' precipitation in pore waters using geochemical modeling. To accomplish these objectives, representative samples from both fresh and oxidized zones were collected along a vertical profile through the tailings pile. Physical, chemical (ICP-MS), and mineralogical characterization (X-ray diffraction (XRD), reflected light microscopy, scanning electron microscope (SEM)) of these samples was performed. Grain size analysis shows that the tailings are dominated by silt- to sand-sized fractions. The microscopic observation highlights the presence of pyrite, marcasite, galena, and sphalerite as primary minerals in a carbonated matrix. The study reveals also the presence of secondary minerals represented by cerussite, smithsonite, anglesite, and Fe oxi-hydroxides as important scavengers for trace elements. The static tests show that the presence of calcite in the tailing samples ensures acid-neutralizing capacity (ANC), which is significantly greater than the acidity potential (PA). The geochemical characterization of the unoxidized samples shows higher Cd, Pb, and Zn concentrations than the oxidized samples containing the highest values for Fe and SO4. Sequential extraction tests show that significant percentages of metals are distributed between the acid-soluble fractions (Cd, Pb, and Zn) and the reducible one (Zn). Pore water analysis indicates that Ca is the dominant cation (8,170 and 6,200 mg L(-1), respectively), whereas sulfate is the principal anion (6,900 and 5,100 mg L(-1), respectively). Saturation index (SI) calculations of minerals in pore water extracted from both the oxidized and unoxidized samples are indicative of gypsum (SI >0) and Fe(III) oxides (SI ≫0) precipitation. The latter controls the Fe concentration in solution.

Theory of Mind and Selective Attention, Response Inhibition, Cognitive Flexibility in Patients with Schizophrenia.

PubMed

Eşsizoğlu, Altan; Köşger, Ferdi; Akarsu, Ferdane Özlem; Özaydin, Özer; Güleç, Gülcan

2017-06-01

The aims of the current study are to investigate the relationship between selective attention, response inhibition, and cognitive flexibility that are among executive functions and sociocognitive and socioperceptual theory of mind (ToM) functions and also to investigate whether selective attention, response inhibition, and cognitive flexibility are predictive factors for ToM functions in patients with schizophrenia. Forty-seven patients diagnosed with schizophrenia and a control group consisting of 42 individuals were administered demographic information form, Wisconsin card sorting test (WCST), Stroop test, Eye test, Hinting test. Positive and negative syndrome scale was applied to the schizophrenia group. In comparison to the control group, the schizophrenia group performed significantly worse on Eyes test and Hinting test. Eyes Test score and age, WCST perseverative error scores were significantly negatively correlated; education and WCST categories achieved scores were significantly positively correlated in patients with schizophrenia. Age and cognitive flexibility were found to predict the Eyes test score in patients with schizophrenia. ToM functions that are important in maintaining socioperceptual functioning are closely related with cognitive flexibility, and impairment in cognitive flexibility may predict the ToM functions in patients with schizophrenia.

Antagonists to TRPV1, ASICs and P2X have a potential role to prevent the triggering of regional bone metabolic disorder and pain-like behavior in tail-suspended mice.

PubMed

Hanaka, Megumi; Iba, Kousuke; Dohke, Takayuki; Kanaya, Kumiko; Okazaki, Shunichiro; Yamashita, Toshihiko

2018-05-01

Our recent studies demonstrated that regional bone loss in the unloaded hind limbs of tail-suspended mice triggered pain-like behaviors due to the acidic environment in the bone induced by osteoclast activation. The aims of the present study were to examine whether TRPV1, ASIC and P2X (known as nociceptors) are expressed in bone, and whether the antagonists to those receptors affect the expression of osteoblast and osteoclast regulators, and prevent the triggering of not only pain-like behaviors but also high bone turnover conditions in tail-suspension model mice. The hind limb-unloaded mice were subjected to tail suspension with the hind limbs elevated for 14days. The effects of the TRPV1, ASIC3, P2X2/3 antagonists on pain-like behaviors as assessed by the von Frey test, paw flick test and spontaneous pain scale; the expressions of TRPV1, ASICs, and P2X2 in the bone; and the effects of those antagonists on osteoblast and osteoclast regulators were examined. In addition, we evaluated the preventive effect of continuous treatment with a TRPV1 antagonist on the trigger for pain-like behavior and bone loss in tail-suspended mice. Pain-like behaviors were significantly improved by the treatment with TRPV1, ASIC, P2X antagonists; TRPV1, ASICs and P2X were expressed in the bone tissues; and the antagonists to these receptors down-regulated the expression of osteoblast and osteoclast regulators in tail-suspended mice. In addition, continuous treatment with a TRPV1 antagonist during tail-suspension prevented the induction of pain-like behaviors and regional bone loss in the unloaded hind limbs. We, therefore, believe that those receptor antagonists have a potential role in preventing the triggering of skeletal pain with associated regional bone metabolic disorder. Copyright © 2018 Elsevier Inc. All rights reserved.

Analgesic effect of piracetam on peripheral neuropathic pain induced by chronic constriction injury of sciatic nerve in rats.

PubMed

Mehta, Ashish K; Bhati, Yogendra; Tripathi, Chakra D; Sharma, Krishna K

2014-08-01

Despite immense advances in the treatment strategies, management of neuropathic pain remains unsatisfactory. Piracetam is a prototype of nootropic drugs, used to improve cognitive impairment. The present study was designed to investigate the effect of piracetam on peripheral neuropathic pain in rats. Neuropathic pain was induced by the chronic constriction injury of the sciatic nerve. Following this, piracetam was intraperitoneally administered for 2 weeks in doses of 50, 100 and 200 mg/kg, and pain was assessed by employing the behavioural tests for thermal hyperalgesia (hot plate and tail flick tests) and cold allodynia (acetone test). After the induction of neuropathic pain, significant development of thermal hyperalgesia and cold allodynia was observed. The administration of piracetam (50 mg/kg) did not have any significant effect on all the behavioural tests. Further, piracetam (100 mg/kg) also had no effect on the hot plate and tail flick tests; however it significantly decreased the paw withdrawal duration in the acetone test. Piracetam in a dose of 200 mg/kg significantly modulated neuropathic pain as observed from the increased hot plate and tail flick latencies, and decreased paw withdrawal duration (in acetone test). Therefore, the present study suggests the potential use of piracetam in the treatment of neuropathic pain, which merits further clinical investigation.

Does climate have heavy tails?

NASA Astrophysics Data System (ADS)

Bermejo, Miguel; Mudelsee, Manfred

2013-04-01

When we speak about a distribution with heavy tails, we are referring to the probability of the existence of extreme values will be relatively large. Several heavy-tail models are constructed from Poisson processes, which are the most tractable models. Among such processes, one of the most important are the Lévy processes, which are those process with independent, stationary increments and stochastic continuity. If the random component of a climate process that generates the data exhibits a heavy-tail distribution, and if that fact is ignored by assuming a finite-variance distribution, then there would be serious consequences (in the form, e.g., of bias) for the analysis of extreme values. Yet, it appears that it is an open question to what extent and degree climate data exhibit heavy-tail phenomena. We present a study about the statistical inference in the presence of heavy-tail distribution. In particular, we explore (1) the estimation of tail index of the marginal distribution using several estimation techniques (e.g., Hill estimator, Pickands estimator) and (2) the power of hypothesis tests. The performance of the different methods are compared using artificial time-series by means of Monte Carlo experiments. We systematically apply the heavy tail inference to observed climate data, in particular we focus on time series data. We study several proxy and directly observed climate variables from the instrumental period, the Holocene and the Pleistocene. This work receives financial support from the European Commission (Marie Curie Initial Training Network LINC, No. 289447, within the 7th Framework Programme).

Analysis of the wind tunnel test of a tilt rotor power force model

NASA Technical Reports Server (NTRS)

Marr, R. L.; Ford, D. G.; Ferguson, S. W.

1974-01-01

Two series of wind tunnel tests were made to determine performance, stability and control, and rotor wake interaction on the airframe, using a one-tenth scale powered force model of a tilt rotor aircraft. Testing covered hover (IGE/OCE), helicopter, conversion, and airplane flight configurations. Forces and moments were recorded for the model from predetermined trim attitudes. Control positions were adjusted to trim flight (one-g lift, pitching moment and drag zero) within the uncorrected test data balance accuracy. Pitch and yaw sweeps were made about the trim attitudes with the control held at the trimmed settings to determine the static stability characteristics. Tail on, tail off, rotors on, and rotors off configurations were testes to determine the rotor wake effects on the empennage. Results are presented and discussed.

Subsonic and supersonic longitudinal stability and control characteristics of an aft tail fighter configuration with cambered and uncambered wings and uncambered fuselage

NASA Technical Reports Server (NTRS)

Dollyhigh, S. M.

1974-01-01

An investigation has been made in the Mach number range from 0.20 to 2.16 to determine the longitudinal aerodynamic characteristics of a fighter airplane concept. The configuration concept employs a single fixed geometry inlet, a 50 deg leading-edge-angle clipped-arrow wing, a single large vertical tail, and low horizontal tails. The wing camber surface was optimized in drag due to lift and was designed to be self-trimming at Mach 1.40 and at a lift coefficient of 0.20. An uncambered or flat wing of the same planform and thickness ratio was also tested. However, for the present investigation, the fuselage was not cambered. Further tests should be made on a cambered fuselage version, which attempts to preserve the optimum wing loading on that part of the theoretical wing enclosed by the fuselage.

Differential Canard deflection for generation of yawing moment on the X-31 with and without the vertical tail. M.S. Thesis - George Washington Univ.

NASA Technical Reports Server (NTRS)

Whiting, Matthew Robert

1996-01-01

The feasibility of augmenting the available yaw control power on the X-31 through differential deflection of the canard surfaces was studied as well as the possibility of using differential canard control to stabilize the X-31 with its vertical tail removed. Wind-tunnel tests and the results of departure criteria and linear analysis showed the destabilizing effect of the reduction of the vertical tail on the X-31. Wind-tunnel testing also showed that differential canard deflection was capable of generating yawing moments of roughly the same magnitude as the thrust vectoring vanes currently in place on the X-31 in the post-stall regime. Analysis showed that the X-31 has sufficient aileron roll control power that with the addition of differential canard as a yaw controller, the wind-axis roll accelerations will remain limited by yaw control authority. It was demonstrated, however, that pitch authority may actually limit the maximum roll rate which can be sustained. A drop model flight test demonstrated that coordinated, wind axis rolls could be performed with roll rates as high as 50 deg/sec (full scale equivalent) at 50 deg angle of attack. Another drop model test was conducted to assess the effect of vertical tail reduction, and an analysis of using differential canard deflection to stabilize the tailless X-31 was performed. The results of six-degree-of-freedom, non-linear simulation tests were correlated with the drop model flights. Simulation studies then showed that the tailless X-31 could be controlled at angles of attack at or above 20 deg using differential canard as the only yaw controller.

Evaluation of liners for a uranium-mill tailings disposal site: a status report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Buelt, J.L.; Hale, V.Q.; Barnes, S.M.

1981-05-01

The United States Department of Energy is conducting a program designed to reclaim or stabilize inactive uranium-mill tailings sites. This report presents the status of the Liner Evaluation Program. The purpose of the study was to identify eight prospective lining materials or composites for laboratory testing. The evaluation was performed by 1) reviewing proposed regulatory requirements to define the material performance criteria; 2) reviewing published literature and communicating with industrial and government experts experienced with lining materials and techniques; and 3) characterizing the tailings at three of the sites for calcium concentration, a selection of anions, radionuclides, organic solvents, andmore » acidity levels. The eight materials selected for laboratory testing are: natural soil amended with sodium-saturated montmorillonite (Volclay); locally available clay in conjunction with an asphalt emulsion radon suppression cover; locally available clay in conjunction with a multibarrier radon suppression cover; rubberized asphalt membrane; hydraulic asphalt concrete; chlorosulfonated polyethylene (hypalon) or high-density polyethylene; bentonite, sand and gravel mixture; and catalytic airblown asphalt membrane. The materials will be exposed in test units now being constructed to conditions such as wet/dry cycles, temperature cycles, oxidative environments, ion-exchange elements, etc. The results of the tests will identify the best material for field study. The status report also presents the information gathered during the field studies at Grand Junction, Colorado. Two liners, a bentonite, sand and gravel mixture, and a catalytic airblown asphalt membrane, were installed in a prepared trench and covered with tailings. The liners were instrumented and are being monitored for migration of moisture, radionuclides, and hazardous chemicals. The two liner materials will also be subjected to accelerated laboratory tests for a comparative assessment.« less

Aerofoil testing in a self-streamlining flexible walled wind tunnel. Ph.D. Thesis - Jul. 1987

NASA Technical Reports Server (NTRS)

Lewis, Mark Charles

1988-01-01

Two-dimensional self-streamlining flexible walled test sections eliminate, as far as experimentally possible, the top and bottom wall interference effects in transonic airfoil testing. The test section sidewalls are rigid, while the impervious top and bottom walls are flexible and contoured to streamline shapes by a system of jacks, without reference to the airfoil model. The concept of wall contouring to eliminate or minimize test section boundary interference in 2-D testing was first demonstrated by NPL in England during the early 40's. The transonic streamlining strategy proposed, developed and used by NPL has been compared with several modern strategies. The NPL strategy has proved to be surprisingly good at providing a wall interference-free test environment, giving model performance indistinguishable from that obtained using the modern strategies over a wide range of test conditions. In all previous investigations the achievement of wall streamlining in flexible walled test sections has been limited to test sections up to those resulting in the model's shock just extending to a streamlined wall. This work however, has also successfully demonstrated the feasibility of 2-D wall streamlining at test conditions where both model shocks have reached and penetrated through their respective flexible walls. Appropriate streamlining procedures have been established and are uncomplicated, enabling flexible walled test sections to cope easily with these high transonic flows.

Serological activity of white-tail deer against several species of Brucella.

PubMed

Salinas-Meléndez, J A; Martínez-Muñoz, A; Avalos-Ramírez, R; Cerutti-Pereyra, N; Riojas-Valdés, V M

1998-01-01

In Mexico, brucellosis is a widely distributed disease of domesticated ruminants, but its frequency in wild ruminants has not been documented. Since northeast Mexico is the main distribution area of white-tailed deer and has been reported as an area positive for brucellosis in domesticated species, the present study was conducted in order to determine serological activity against several species of the genus Brucella in white-tailed deer. A total of 208 sera of white-tailed deer were collected during the springs of 1994 and 1995 in the north part of the states of Nuevo León and Coahuila. Each serum was analyzed for the detection of antibodies against two smooth (B. abortus and B. melitensis) and one rough (B. ovis) species of the genus Brucella. The serological tests used for the determination of the presence of antibodies against Brucella were card and plate agglutination for B. abortus, plate agglutination and rivanol precipitation for B. melitensis, and agar gel immunodiffusion for B. ovis. Each assay had positive and negative controls. None of the analyzed samples was found to be positive, and only two sera showed partial plate agglutination against B. melitensis at a dilution of 1:25; however, at higher dilutions and to the rivanol precipitation test the same samples were negative. Therefore, the percentage of positive sera was estimated at 0% (0/208). This result makes evident the absence of positive white-tailed deer against Brucella in the sampled area, despite that this disease is considered present in domesticated species. Therefore, white-tailed deer does not have, at the present time, an important role for the dispersion of the disease. The same result has been reported in other countries.

Individual identification of Sitka black-tailed deer (Odocoileus hemionus sitkensis) using DNA from fecal pellets

Treesearch

Todd J. Brinkman; David K. Person; Michael K. Schwartz; Kristine L. Pilgrim; Kevin E. Colson; Kris J. Hundertmark

2010-01-01

We tested a protocol for extracting DNA from fecal pellets from Sitka black-tailed deer (Odocoileus hemionus sitkensis) and evaluated genotyping performance of previously developed microsatellite markers as well as a suite of new markers designed specifically for this study. We screened 30 microsatellites, and identified 7 (23%) loci including 4 new markers, that fit...

Design and evaluation of flexible membrane vesicles (FMVs) for enhanced topical delivery of capsaicin.

PubMed

Raza, Kaisar; Singh, Bhupinder; Mahajan, Anadi; Negi, Poonam; Bhatia, Amit; Katare, O P

2011-05-01

Capsaicin, extracted from the fruits of Capsicum, is a powerful local stimulant with strong rubifacient action, devoid of vesication. Topical use of capsaicin is quite common in the treatment of various pain-associated musculo-skeletal disorders, itching and neuropathy. Despite its high pharmacodynamic potential, the patient compliance to the drug is reported to be poor owing to multiple skin problems like irritation, burning sensation, and erythma. The present study targets the encasement of drug in the interiors of flexible membrane vesicles (FMVs), as these are reported to have better penetration in the deeper layers of skin, thus leading to enhanced localization of drug and consequently, decreased skin irritation. Multilamellar drug-loaded FMVs, prepared by thin-film hydration were evaluated for their efficacy in vitro and in vivo. When compared with conventional liposomes, the formulated FMVs showed higher skin retention during ex vivo permeation studies employing LACA mice skin, higher analgesic potential using radiant tail-flick method in mice, and better flexibility in regaining their size. Being less of an irritant, these vesicular carriers were also found to be more comfortable on human skin. Thus, the capsaicin-loaded FMVs offer high potential as topical drug delivery technologies with improved patient acceptance and effectiveness.

Dogs' Body Language Relevant to Learning Achievement.

PubMed

Hasegawa, Masashi; Ohtani, Nobuyo; Ohta, Mitsuaki

2014-02-27

The facial expressions and body postures of dogs can give helpful information about their moods and emotional states. People can more effectively obedience train their dogs if we can identify the mannerisms associated with learning in dogs. The aim of this study was to clarify the dog's body language during operant conditioning to predict achievement in the test that followed by measuring the duration of behaviors. Forty-six untrained dogs (17 males and 26 females) of various breeds were used. Each session consisted of 5 minutes of training with a treat reward followed by 3 minutes of rest and finally an operant conditioning test that consisted of 20 "hand motion" cues. The operant tests were conducted a total of nine times over three consecutive days, and the success numbers were counted. The duration of the dog's behavior, focusing on the dog's eyes, mouth, ears, tail and tail-wagging, was recorded during the operant conditioning sessions before the test. Particular behaviors, including wide-eyes, closed mouth, erect ears, and forward and high tail carriage, without wagging or with short and quick wagging, related to high achievement results. It is concluded that dogs' body language during operant conditioning was related to their success rate.

Flight Services and Aircraft Access: Active Flow Control Vertical Tail and Insect Accretion and Mitigation Flight Test

NASA Technical Reports Server (NTRS)

Whalen, Edward A.

2016-01-01

This document serves as the final report for the Flight Services and Aircraft Access task order NNL14AA57T as part of NASA Environmentally Responsible Aviation (ERA) Project ITD12A+. It includes descriptions of flight test preparations and execution for the Active Flow Control (AFC) Vertical Tail and Insect Accretion and Mitigation (IAM) experiments conducted on the 757 ecoDemonstrator. For the AFC Vertical Tail, this is the culmination of efforts under two task orders. The task order was managed by Boeing Research & Technology and executed by an enterprise-wide Boeing team that included Boeing Research & Technology, Boeing Commercial Airplanes, Boeing Defense and Space and Boeing Test and Evaluation. Boeing BR&T in St. Louis was responsible for overall Boeing project management and coordination with NASA. The 757 flight test asset was provided and managed by the BCA ecoDemonstrator Program, in partnership with Stifel Aircraft Leasing and the TUI Group. With this report, all of the required deliverables related to management of this task order have been met and delivered to NASA as summarized in Table 1. In addition, this task order is part of a broader collaboration between NASA and Boeing.

Positive human contact on the first day of life alters the piglet's behavioural response to humans and husbandry practices.

PubMed

Muns, Ramon; Rault, Jean-Loup; Hemsworth, Paul

2015-11-01

This experiment examined the effects of positive human contact at suckling on the first day of life on the behavioural and physiological responses of piglets to both humans and routine husbandry procedures. Forty litters from multiparous sows were randomly allocated to one of two treatments: Control (CC, minimal human interaction with day-old piglets) or Positive Contact (PC, human talking and caressing piglets during 6 suckling bouts on their first day of life, day 1). In each litter, 2 males and 2 females were randomly selected and their behavioural responses to tail docking (day 2), and to an experimenter (day 35) were studied. Escape behaviour at tail docking was assessed according to intensity (on a scale from 0 to 4 representing no movement to high intensity movement) and duration (on a scale from 0 to 3 representing no movement to continuous movement). At day 15 of age, a human approach and avoidance test was performed on focal piglets and at day 15, escape behaviour to capture before and after testing was recorded again. Blood samples for cortisol analysis were obtained from the focal piglets 30 min after tail docking and 1 h after weaning. Escape behaviour to tail docking of the PC piglets was of shorter duration than that of the CC piglets (P = 0.05). There was a tendency for the escape behaviour both before and after testing at day 15 to be of a lower intensity (P = 0.11 and P = 0.06, respectively) and a shorter duration (P = 0.06 and P = 0.08, respectively) in the PC piglets. There was a tendency for PC piglets to have higher cortisol concentrations after tail docking than the CC piglets (P = 0.07). Male piglets had higher cortisol concentrations after tail docking and after weaning than female piglets (P = 0.02 and P = 0.03). The results indicate that Positive Contact treatment reduced the duration of escape behaviour of piglets to tail docking. The role of classical conditioning, habituation and developmental changes in the observed effects of the Positive Contact treatment is unclear. Nonetheless, this experiment demonstrated that brief positive human contacts early in life can alter the behavioural responses of piglets to subsequent stressful events.

Correlate Life Predictions and Condition Indicators in Helicopter Tail Gearbox Bearings

NASA Technical Reports Server (NTRS)

Dempsey, Paula J.; Bolander, Nathan; Haynes, Chris; Branning, Jeremy; Wade, Daniel R.

2010-01-01

Research to correlate bearing remaining useful life (RUL) predictions with Helicopter Health Usage Monitoring Systems (HUMS) condition indicators (CI) to indicate the damage state of a transmission component has been developed. Condition indicators were monitored and recorded on UH-60M (Black Hawk) tail gearbox output shaft thrust bearings, which had been removed from helicopters and installed in a bearing spall propagation test rig. Condition indicators monitoring the tail gearbox output shaft thrust bearings in UH-60M helicopters were also recorded from an on-board HUMS. The spal-lpropagation data collected in the test rig was used to generate condition indicators for bearing fault detection. A damage progression model was also developed from this data. Determining the RUL of this component in a helicopter requires the CI response to be mapped to the damage state. The data from helicopters and a test rig were analyzed to determine if bearing remaining useful life predictions could be correlated with HUMS condition indicators (CI). Results indicate data fusion analysis techniques can be used to map the CI response to the damage levels.

Development of weight and cost estimates for lifting surfaces with active controls

NASA Technical Reports Server (NTRS)

Anderson, R. D.; Flora, C. C.; Nelson, R. M.; Raymond, E. T.; Vincent, J. H.

1976-01-01

Equations and methodology were developed for estimating the weight and cost incrementals due to active controls added to the wing and horizontal tail of a subsonic transport airplane. The methods are sufficiently generalized to be suitable for preliminary design. Supporting methodology and input specifications for the weight and cost equations are provided. The weight and cost equations are structured to be flexible in terms of the active control technology (ACT) flight control system specification. In order to present a self-contained package, methodology is also presented for generating ACT flight control system characteristics for the weight and cost equations. Use of the methodology is illustrated.

Notoraja martinezi sp. nov., a new species of deepwater skate and the first record of the genus Notoraja Ishiyama, 1958 (Rajiformes: Arhynchobatidae) from the eastern Pacific Ocean.

PubMed

Concha, Francisco J; Ebert, David A; Long, Douglas J

2016-04-05

A new arhynchobatid skate, Notoraja martinezi, sp. nov., is described from four specimens collected from the eastern Central Pacific from Costa Rica to Ecuador and between depths of 1256-1472 m. The new species is placed in the genus Notoraja based on the long and flexible rostrum and its proportionally long tail with respect to total length. This species is distinct from its congeners in the Western Pacific by the straight margins of its rostrum, long anterior lobes of pelvic fins, and its abundant and very well developed caudal thorns.

Tail Service Mast Umbilical Arrival

NASA Image and Video Library

2016-08-02

A heavy-lift transport truck arrives at the Launch Equipment Test Facility (LETF) at NASA’s Kennedy Space Center in Florida, with the first of two Tail Service Mast Umbilicals (TSMU) for NASA’s Space Launch System (SLS). Two TSMUs will provide liquid propellants and power to the Space Launch System (SLS) rocket’s core stage engine. Both TSMUs will connect to the zero-level deck on the mobile launcher, providing fuel and electricity to the SLS rocket before it launches on Exploration Mission 1. The TSMU will undergo testing and validation at the LETF to verify it is functioning properly. The center’s Engineering Directorate and the Ground Systems Development and Operations Program are overseeing processing and testing of the umbilicals.

Tail Service Mast Umbilical Arrival

NASA Image and Video Library

2016-08-02

Technicians assist as a crane is used to lift the first Tail Service Mast Umbilical (TSMU) into the vertical position at the Launch Equipment Test Facility (LETF) at NASA’s Kennedy Space Center in Florida. Two TSMUs will provide liquid propellants and power to the Space Launch System (SLS) rocket’s core stage engine. Both TSMUs will connect to the zero-level deck on the mobile launcher, providing fuel and electricity to the SLS rocket before it launches on Exploration Mission 1. The TSMU will undergo testing and validation at the LETF to verify it is functioning properly. The center’s Engineering Directorate and the Ground Systems Development and Operations Program are overseeing processing and testing of the umbilicals.

Tail Service Mast Umbilical Arrival

NASA Image and Video Library

2016-08-02

A crane is prepared to help lift the first Tail Service Mast Umbilical (TSMU) for NASA’s Space Launch System (SLS) at the Launch Equipment Test Facility (LETF) at NASA’s Kennedy Space Center in Florida. Two TSMUs will provide liquid propellants and power to the Space Launch System (SLS) rocket’s core stage engine. Both TSMUs will connect to the zero-level deck on the mobile launcher, providing fuel and electricity to the SLS rocket before it launches on Exploration Mission 1. The TSMU will undergo testing and validation at the LETF to verify it is functioning properly. The center’s Engineering Directorate and the Ground Systems Development and Operations Program are overseeing processing and testing of the umbilicals.

Tail Service Mast Umbilical Arrival

NASA Image and Video Library

2016-08-02

A crane is attached to the first Tail Service Mast Umbilical (TSMU) for NASA’s Space Launch System (SLS) at the Launch Equipment Test Facility at NASA’s Kennedy Space Center in Florida. Two TSMUs will provide liquid propellants and power to the Space Launch System (SLS) rocket’s core stage engine. Both TSMUs will connect to the zero-level deck on the mobile launcher, providing fuel and electricity to the SLS rocket before it launches on Exploration Mission 1. The TSMU will undergo testing and validation at the LETF to verify it is functioning properly. The center’s Engineering Directorate and the Ground Systems Development and Operations Program are overseeing processing and testing of the umbilicals.

On the identification of Dragon Kings among extreme-valued outliers

NASA Astrophysics Data System (ADS)

Riva, M.; Neuman, S. P.; Guadagnini, A.

2013-07-01

Extreme values of earth, environmental, ecological, physical, biological, financial and other variables often form outliers to heavy tails of empirical frequency distributions. Quite commonly such tails are approximated by stretched exponential, log-normal or power functions. Recently there has been an interest in distinguishing between extreme-valued outliers that belong to the parent population of most data in a sample and those that do not. The first type, called Gray Swans by Nassim Nicholas Taleb (often confused in the literature with Taleb's totally unknowable Black Swans), is drawn from a known distribution of the tails which can thus be extrapolated beyond the range of sampled values. However, the magnitudes and/or space-time locations of unsampled Gray Swans cannot be foretold. The second type of extreme-valued outliers, termed Dragon Kings by Didier Sornette, may in his view be sometimes predicted based on how other data in the sample behave. This intriguing prospect has recently motivated some authors to propose statistical tests capable of identifying Dragon Kings in a given random sample. Here we apply three such tests to log air permeability data measured on the faces of a Berea sandstone block and to synthetic data generated in a manner statistically consistent with these measurements. We interpret the measurements to be, and generate synthetic data that are, samples from α-stable sub-Gaussian random fields subordinated to truncated fractional Gaussian noise (tfGn). All these data have frequency distributions characterized by power-law tails with extreme-valued outliers about the tail edges.

Naive, captive long-tailed macaques (Macaca fascicularis fascicularis) fail to individually and socially learn pound-hammering, a tool-use behaviour

PubMed Central

Tennie, Claudio

2018-01-01

A subspecies of long-tailed macaques (Macaca fascicularis aurea; Mfa) has been reported to use stone tools and a specific technique to process nuts in Southeast Asia, a behaviour known as ‘pound-hammering’. The aim of this study was to examine the development of pound-hammering in long-tailed macaques: whether this behavioural form can be individually learnt or whether it has to rely on some forms of social learning. Given the absence of Mfa from captivity, long-tailed macaques of a highly related subspecies (Macaca fascicularis fascicularis; Mff) were experimentally tested by providing them with the ecological materials necessary to show pound-hammering. A baseline was first carried out to observe whether pound-hammering would emerge spontaneously without social information. As this was not the case, different degrees of social information, culminating in a full demonstration of the behaviour, were provided. None of the subjects (n = 31) showed pound-hammering in any of the individual or social learning conditions. Although these data do not support the hypothesis that individual learning underlies this behaviour, no evidence was found that (at least) Mff learn pound-hammering socially either. We propose that other—potentially interacting—factors may determine whether this behaviour emerges in the various subspecies of long-tailed macaques, and provide a novel methodology to test the role of social and individual learning in the development of animal tool-use. PMID:29892375

Rotary balance data for a typical single-engine general aviation design for an angle-of-attack range of 8 deg to 90 deg. 2: Influence of horizontal tail location for Model D

NASA Technical Reports Server (NTRS)

Barnhart, B.

1982-01-01

The influence of horizontal tail location on the rotational flow aerodynamics is discussed for a 1/6-scale general aviation airplane model. The model was tested using various horizontal tail positions, with both a high and a low-wing location and for each of two body lengths. Data were measured, using a rotary balance, over an angle-of-attack range of 8 to 90 deg, and for clockwise and counter-clockwise rotations covering an Omega b/2V range of 0 to 0.9.

Fatal West Nile virus infection in a white-tailed deer (Odocoileus virginianus).

PubMed

Miller, Debra L; Radi, Zaher A; Baldwin, Charles; Ingram, Dallas

2005-01-01

A 3-yr-old male white-tailed deer (Odocoileus virginianus) with a history of ataxia and tremors was submitted to the Tifton Veterinary Diagnostic and Investigational Laboratory (The University of Georgia, Tifton, Georgia, USA) for necropsy. Gross findings were unremarkable. Histologically, the brain had multifocal lymphoplasmacytic perivascular inflammation, scattered gliosis, and rare satellitosis. Mild hemorrhage and congestion in the retropharyngeal lymph nodes and mild lymphoid depletion in the tonsil were present. A reverse transcription-polymerase chain reaction test performed on brain yielded a positive result for West Nile virus. This represents the first report of fatal West Nile virus infection in a white-tailed deer.

The Validity of the Modified Sit-and-Reach Test in College-Age Students.

ERIC Educational Resources Information Center

Minkler, Sharin; Patterson, Patricia

1994-01-01

Reports a study that examined the criterion-related validity of the modified sit-and-reach test against criterion measures of hamstring and low back flexibility in college students. Results indicated the modified sit-and-reach test moderately related to hamstring flexibility, but its relation to low back flexibility was low. (SM)

Effect of Bearing Defects

DTIC Science & Technology

1974-12-01

defect types were tested at various levels: Comet- Tail, Dig-Nick, Dirt Brinell, Grind-Skip Lines, Impingement, Orange Peel , Pit, Scratch and "Liney...Shallow irregular indentation of surface. <.0015 max. dim. -(<.0008)*’ Otange Peel Pebbly appearance of raceway surface. Small ** Comet Tail Pit...scratch; dig-nick; impingement; grind-skip lines; and orange peel . The data obtained indicated that these defects in most cases, affected bearing

Monolayers of the lipid derivatives of isoniazid at the air/water interface and the formation of self-assembled nanostructures in water.

PubMed

Jin, Yiguang; Chen, Shufeng; Xin, Rui; Zhou, Yisheng

2008-07-15

Isoniazid (INH, isonicotinic acid hydrazide) is one of the most commonly used anti-tubercular drugs. However, resistance of Mycobacterium tuberculosis strains to anti-mycobacterial agents including INH is an increasing problem worldwide. Development of new anti-mycobacterial agents thus has attracted attention. Five lipid derivatives of INH were prepared in this study. They formed monolayers at the air/water interface, and some nanostructures with different morphologies were obtained through molecular self-assembly in water. The derivatives included one fatty acyl derivative containing a 12-C hydrocarbon-long chain (1), three fatty alcohol derivatives with a succinyl as spacer and an 8, 12 or 16-C hydrocarbon-long chain (2, 3 and 4), and one tetrahydro-2H-1,3,5-thiadiazine-2-thione (THTT) derivative containing a 12-C hydrocarbon-long chain (5). The surface pressure-area isotherms depended on the volume and configuration of heads and the length of tails of derivatives. Compound 2 had a relatively large head and a short tail, easily standing uprightly at the interface. Under a certain surface pressure, the linear polar head groups of 3 could be partly squeezed out and insert into subphase because the length of heads were comparable to the one of tails. The very long tails of 4 always maintained above the interface and led to a high collapse pressure. Compound 5 possessed an extended and large head consisting of the THTT and INH groups so that the relatively short tails tilted at the interface and difficultly contact with each other. The THTT rings might be partly squeezed out and enter into air under a certain surface pressure. The self-assembly behaviours of derivatives in water depended on the molecular configuration and agreed with the corresponding monolayer behaviours. The flexible and medium-long tails (1 and 3) led to the derivatives to form nanoscale vesicles, though the short or very long tails did not (2 and 4). Interestingly, intermolecular hydrogen bonding could occur between the molecules of 5, and improve the derivative forming helical nanofibres other than vesicles. The molecular self-assembly of INH's lipid derivatives was explored in details in this study. The formation mechanisms of self-assembled nanostructures were analyzed. Various types of self-assembled nanostructures were obtained and the formation mechanisms were analyzed. The relationship between the self-assembly and the molecular configuration of amphiphilic derivatives was also revealed. The lipid derivatives of INH show promising anti-Mycobacterium action because the amphiphilic prodrugs allow for better penetration of the bacterial cells. The self-assembled nanostructures may likely be the potential self-assembled drug delivery systems for tuberculosis therapy.

30 x 60 foot wind tunnel test highlights for an over-the-tail advanced turboprop configuration

NASA Technical Reports Server (NTRS)

Coe, Paul L., Jr.; Perkins, John N.; Rhodes, Graham S.

1991-01-01

This paper presents results from a recent investigation of the static aerodynamic and stability characteristics of a two-surface advanced turboprop aircraft. The conceptual design places Hamilton Standard SR-7 turboprop blades close to the horizontal and vertical tail for potential acoustic shielding. Evaluation of the data shows generally favorable effects of power on aircraft stability and control, and that lateral directional trim can be achieved with one engine inoperative. The tests did show a marked effect of the direction of propeller rotation on thrust minus drag performance.

Kinetics of veratridine action on Na channels of skeletal muscle

PubMed Central

Sutro, JB

1986-01-01

Veratridine bath-applied to frog muscle makes inactivation of INa incomplete during a depolarizing voltage-clamp pulse and leads to a persistent veratridine-induced Na tail current. During repetitive depolarizations, the size of successive tail currents grows to a plateau and then gradually decreases. When pulsing is stopped, the tail current declines to zero with a time constant of approximately 3 s. Higher rates of stimulation result in a faster build-up of the tail current and a larger maximum value. I propose that veratridine binds only to open channels and, when bound, prevents normal fast inactivation and rapid shutting of the channel on return to rest. Veratridine-modified channels are also subject to a "slow" inactivation during long depolarizations or extended pulse trains. At rest, veratridine unbinds with a time constant of approximately 3 s. Three tests confirm these hypotheses: (a) the time course of the development of veratridine-induced tail currents parallels a running time integral of gNa during the pulse; (b) inactivating prepulses reduce the ability to evoke tails, and the voltage dependence of this reduction parallels the voltage dependence of h infinity; (c) chloramine-T, N-bromoacetamide, and scorpion toxin, agents that decrease inactivation in Na channels, each greatly enhance the tail currents and alter the time course of the appearance of the tails as predicted by the hypothesis. Veratridine-modified channels shut during hyperpolarizations from -90 mV and reopen on repolarization to -90 mV, a process that resembles normal activation gating. Veratridine appears to bind more rapidly during larger depolarizations. PMID:2419478

The effect of oil sands tailings pond sediments on embryo-larval walleye (Sander vitreus).

PubMed

Raine, J C; Turcotte, D; Tumber, V; Peru, K M; Wang, Z; Yang, C; Headley, J V; Parrott, J L

2017-10-01

Walleye (Sander vitreus) are a commercially important North American fish species that inhabit the Athabasca River. This river flows through the Athabasca oil sands where natural sources of bitumen erode from the McMurray formation. Little information is available on responses of walleye embryos to oil sands tailings pond sediments in a laboratory setting. The current study describes the design and implementation of a daily-renewal bioassay to assess the potential effects of tailings pond sediments from the Athabasca oil sands area on walleye development. Developing walleye embryos were exposed to increasing concentrations of two tailings pond sediments (collected in the Athabasca oil sands area) until the completion of yolk absorption in control fish. Sediments from the tailings pond represent a mixture of polycyclic aromatic hydrocarbons (PAHs) and alkylated PAHs. During the 31 day exposure, the walleye were examined for mortalities, weight, length and developmental abnormalities to provide an initial evaluation of the effects of the oil sands tailings pond sediments. Walleye embryo survival differed between the tailings pond sediments, and survival decreased with increasing sediment concentration. Alkylated PAH content differed between the two tailings pond sediments and lower embryo survival corresponded to higher total and alkylated PAH content. Tailings pond sediment-exposed walleye exhibited a delay in development, as well as increased percentages of larvae with heart and yolk sac edema, and cranial and spinal malformations. These abnormalities in development are often associated with PAH and alkylated PAH exposure. This study provides an exposure design that can be used to assess sediment toxicity to early developmental stages of a fish species not commonly tested in the lab, and lays the groundwork for future studies with this and other difficult-to-culture species. These results offer information on the potential effects of tailings pond sediments containing PAH/alkylated PAH mixtures on walleye development and survival. Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

Time-varying motor control of autotomized leopard gecko tails: multiple inputs and behavioral modulation.

PubMed

Higham, Timothy E; Russell, Anthony P

2012-02-01

Autotomy (voluntary loss of an appendage) is common among diverse groups of vertebrates and invertebrates, and much attention has been given to ecological and developmental aspects of tail autotomy in lizards. Although most studies have focused on the ramifications for the lizard (behavior, biomechanics, energetics, etc.), the tail itself can exhibit interesting behaviors once segregated from the body. For example, recent work highlighted the ability of leopard gecko tails to jump and flip, in addition to being able to swing back and forth. Little is known, however, about the control mechanisms underlying these movements. Using electromyography, we examined the time-varying in vivo motor patterns at four sites (two proximal and two distal) in the tail of the leopard gecko, Eublepharis macularius, following autotomy. Using these data we tested the hypothesis that the disparity in movements results simply from overlapping pattern generators within the tail. We found that burst duration, but not cycle duration, of the rhythmic swings reached a plateau at approximately 150 s following autotomy. This is likely because of physiological changes related to muscle fatigue and ischemia. For flips and jumps, burst and cycle duration exhibited no regular pattern. The coefficient of variation in motor patterns was significantly greater for jumps and flips than for rhythmic swings. This supports the conclusion that the different tail behaviors do not stem from overlapping pattern generators, but that they rely upon independent neural circuits. The signal controlling jumps and flips may be modified by sensory information from the environment. Finally, we found that jumps and flips are initiated using relatively synchronous activity between the two sides of the tail. In contrast, alternating activation of the right and left sides of the tail result in rhythmic swings. The mechanism underlying this change in tail behavior is comparable to locomotor gait changes in vertebrates.

Microgravity-induced modifications of the vestibuloocular reflex in Xenopus laevis tadpoles are related to development and the occurrence of tail lordosis.

PubMed

Horn, Eberhard R

2006-08-01

During space flights, tadpoles of the clawed toad Xenopus laevis occasionally develop upward bended tails (tail lordosis). The tail lordosis disappears after re-entry to 1g within a couple of days. The mechanisms responsible for the induction of the tail lordosis are unknown; physical conditions such as weight de-loading or physiological factors such as decreased vestibular activity in microgravity might contribute. Microgravity (microg) also exerts significant effects on the roll-induced vestibuloocular reflex (rVOR). The rVOR was used to clarify whether tail lordosis is caused by physiological factors, by correlating the occurrence of microg-induced tail lordosis with the extent of microg-induced rVOR modifications. Post-flight recordings from three space flights (D-2 Spacelab mission, STS-55 in 1993; Shuttle-to-Mir mission SMM-06, STS-84 in 1997; French Soyuz taxi flight Andromède to ISS in 2001) were analyzed in these experiments. At onset of microgravity, tadpoles were at stages 25-28, 33-36 or 45. Parameters tested were rVOR gain (ratio between the angular eye movement and the lateral 30 degrees roll) and rVOR amplitude (maximal angular postural change of the eyes during a 360 degrees lateral roll). A ratio of 22-84% of tadpoles developed lordotic tails, depending on the space flight. The overall observation was that the rVOR of tadpoles with normal tails was either not affected by microgravity, or it was enhanced. In contrast, the rVOR of lordotic animals always revealed a depression. In particular, during post-flight days 1-11, tadpoles with lordotic tails from all three groups (25-28, 33-36 and 45) showed a lower rVOR gain and amplitude than the 1g-controls. The rVOR gain and amplitude of tadpoles from the groups 25-28 and 33-36 that developed normal tails was not affected by microgravity while the rVOR of microg-tadpoles from the stage-45 group with normal tails revealed a significant rVOR augmentation. (1) the vestibular system of tadpoles with lordotic tails is developmentally retarded by microgravity; (2) after a critical status of vestibular maturation obtained during the appearance of first swimming, microgravity activates an adaptation mechanism that causes a sensitization of the vestibular system.

Flexible Transcription Testing

ERIC Educational Resources Information Center

Carr-Smith, Norma

1976-01-01

Flexible structure in a San Francisco State University shorthand course is described as a way to provide motivation for students. Topics discussed are transcription testing plan, method of evaluation, practice tests, increasing difficulty of tests, and classroom results. (TA)

RLV-TD Flight Measured Aeroacoustic Levels and its Comparison with Predictions

NASA Astrophysics Data System (ADS)

Manokaran, K.; Prasath, M.; Venkata Subrahmanyam, B.; Ganesan, V. R.; Ravindran, Archana; Babu, C.

2017-12-01

The Reusable Launch Vehicle-Technology Demonstrator (RLV-TD) is a wing body configuration successfully flight tested. One of the important flight measurements is the acoustic levels. There were five external microphones, mounted on the fuselage-forebody, wing, vertical tail, inter-stage (ITS) and core base shroud to measure the acoustic levels from lift-off to splash down. In the ascent phase, core base shroud recorded the overall maximum at both lift-off and transonic conditions. In-flight noise levels measured on the wing is second highest, followed by fuselage and vertical tail. Predictions for flight trajectory compare well at all locations except for vertical tail (4.5 dB). In the descent phase, maximum measured OASPL occurs at transonic condition for the wing, followed by vertical tail and fuselage. Predictions for flight trajectory compare well at all locations except for wing (- 6.0 dB). Spectrum comparison is good in the ascent phase compared to descent phase. Roll Reaction control system (RCS) thruster firing signature is seen in the acoustic measurements on the wing and vertical tail during lift-off.

Leaching behavior of U, Mn, Sr, and Pb from different particle-size fractions of uranium mill tailings.

PubMed

Liu, Bo; Peng, Tongjiang; Sun, Hongjuan

2017-06-01

Pollution by the release of heavy metals from tailings constitutes a potential threat to the environment. To characterize the processes governing the release of Mn, Sr, Pb, and U from the uranium mill tailings, a dynamic leaching test was applied for different size of uranium mill tailings samples. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) and inductively coupled plasma mass spectrometry (ICP-MS) were performed to determine the content of Mn, Sr, Pb, and U in the leachates. The release of mobile Mn, Sr, Pb, and U fraction was slow, being faster in the initial stage and then attained a near steady-state condition. The experimental results demonstrate that the release of Mn, Sr, Pb, and U from uranium mill tailings with different size fractions is controlled by a variety of mechanisms. Surface wash-off is the release mechanism for Mn. The main release mechanism of Sr and Pb is the dissolution in the initial leaching stage. For U, a mixed process of wash-off and diffusion is the controlling mechanism.

The functional link between tail-pinch-induced food intake and emotionality and its possible role in stress coping in rats.

PubMed

Aso-Someya, Nami; Narikiyo, Kimiya; Masuda, Akira; Aou, Shuji

2018-02-08

Tail pinch facilitates eating in rats. We investigated an unidentified link between tail-pinch-induced eating behavior and individual emotionality in male Sprague-Dawley rats. Anxiety-like behavior was assessed on the elevated plus maze (EPM) and in the open field test (OFT). Tail-pinch-induced eating was observed as follows: After a 30-min habituation period, the tail pinch was applied for 5 min, followed by a 30-min recovery period. During the habituation and recovery periods, rats were allowed to access food ad libitum. During the recovery period, 14 of 24 rats ate more food than during the habituation period. Thus, we named them "high responders" and the others as "low responders". The food intake was significantly greater, while the times spent in the open arms in the EPM and in the center area in the OFT were significantly shorter in high responders than in low responders. This result suggests that the rats consuming more food after mild stress have higher anxiety.

An Aspergillus aculateus strain was capable of producing agriculturally useful nanoparticles via bioremediation of iron ore tailings.

PubMed

Bedi, Ankita; Singh, Braj Raj; Deshmukh, Sunil K; Adholeya, Alok; Barrow, Colin J

2018-06-01

Mining waste such as iron ore tailing is environmentally hazardous, encouraging researchers to develop effective bioremediation technologies. Among the microbial isolates collected from iron ore tailings, Aspergillus aculeatus (strain T6) showed good leaching efficiency and produced iron-containing nanoparticles under ambient conditions. This strain can convert iron ore tailing waste into agriculturally useful nanoparticles. Fourier-transform Infrared Spectroscopy (FT-IR analysis) established the at the particles are protein coated, with energy dispersive X-ray Spectroscopy (EDX analysis) showing strong signals for iron. Transmission Electron Microscopy (TEM analysis) showed semi-quasi spherical particles having average size of 15 ± 5 nm. These biosynthesized nanoparticles when tested for their efficacy on seed emergence activity of mungbean (Vigna radiata) seeds, and enhanced plant growth at 10 and 20 ppm. Copyright © 2018 Elsevier Ltd. All rights reserved.

LDV Surveys Over a Fighter Model at Moderate to High Angles of Attack

NASA Technical Reports Server (NTRS)

Sellers, William L., III; Meyers, James F.; Hepner, Timothy E.

2004-01-01

The vortex flowfield over an advanced twin-tailed fighter configuration was measured in a low-speed wind tunnel at two angles of attack. The primary test data consisted of 3-component velocity surveys obtained using a Laser Doppler Velocimeter. Laser light sheet and surface flow visualization were also obtained to provide insight into the flowfield structure. Time-averaged velocities and the root mean square of the velocity fluctuations were obtained at two cross-sections above the model. At 15 degrees angle of attack, the vortices generated by the wing leading edge extension (LEX) were unburst over the model and passed outboard of the vertical tail. At 25 degrees angle of attack, the vortices burst in the vicinity of the wing-LEX intersection and impact directly on the vertical tails. The RMS levels of the velocity fluctuations reach values of approximately 30% in the region of the vertical tails.

Aerodynamic characteristics of a canard-controlled missile at Mach numbers of 1.5 and 2.0.

NASA Technical Reports Server (NTRS)

Kassner, D. L.; Wettlaufer, B.

1977-01-01

A typical missile model with nose mounted canards and cruciform tail surfaces was tested in the Ames 6- by 6-Foot Wind Tunnel to determine the contributions of the component aerodynamic surfaces to the static aerodynamic characteristics at Mach numbers of 1.5 and 2.0 and Reynolds number of 1 million based on body diameter. Data were obtained at angles of attack ranging from -3 deg to 12 deg for various stages of model build-up (i.e., with and without canard and/or tail surfaces). Results were obtained both with the model unrolled and rolled 45 deg. For the canard and tail arrangements investigated, the model was trimmable at angles of attack up to about 10 deg with canard deflections of 9 deg. Also, the tail arrangements studied provided ample pitch stability. there were no appreciable effects of model roll orientation.

Cruise and turning performance of an improved fish robot actuated by piezoceramic actuators

NASA Astrophysics Data System (ADS)

Nguyen, Quang Sang; Heo, Seok; Park, Hoon Cheol; Goo, Nam Seo; Byun, Doyoung

2009-03-01

The purpose of this study is improvement of a fish robot actuated by four light-weight piezocomposite actuators (LIPCAs). In the fish robot, we developed a new actuation mechanism working without any gear and thus the actuation mechanism was simple in fabrication. By using the new actuation mechanism, cross section of the fish robot became 30% smaller than that of the previous model. Performance tests of the fish robot in water were carried out to measure tail-beat angle, thrust force, swimming speed and turning radius for tail-beat frequencies from 1Hz to 5Hz. The maximum swimming speed of the fish robot was 7.7 cm/s at 3.9Hz tail-beat frequency. Turning experiment showed that swimming direction of the fish robot could be controlled with 0.41 m turning radius by controlling tail-beat angle.

Congenital transmission of Neospora caninum in white-tailed deer (Odocoileus virginianus).

PubMed

Dubey, J P; Jenkins, M C; Kwok, O C H; Ferreira, L R; Choudhary, S; Verma, S K; Villena, I; Butler, E; Carstensen, M

2013-09-23

Neosporosis is an important cause of bovine abortion worldwide. Many aspects of transmission of Neospora caninum in nature are unknown. The white-tailed deer (Odocoileus virginianus) is considered one of the most important wildlife reservoirs of N. caninum in the USA. During the hunting seasons of 2008, 2009, and 2010, brains of 155 white-tailed deer fetuses were bioassayed in mice for protozoal isolation. Viable N. caninum (NcWTDMn1, NcWTDMn2) was isolated from the brains of two fetuses by bioassays in mice, and subsequent propagation in cell culture. Dams of these two infected fetuses had antibodies to N. caninum by Neospora agglutination test at 1:100 serum dilution. DNA obtained from culture-derived N. caninum tachyzoites of the two isolates with Nc5 PCR confirmed diagnosis. Results prove congenital transmission of N. caninum in the white tailed deer for the first time. Published by Elsevier B.V.

Effect of anxiolytics on cognitive flexibility in problem solving.

PubMed

Silver, Jennifer A; Hughes, John D; Bornstein, Robert A; Beversdorf, David Q

2004-06-01

Our purpose is to examine the effect of different classes of anxiolytics on cognitive flexibility. Situational stressors and anxiety impede performance on "creativity" tests requiring cognitive flexibility. Noradrenergic agents have been shown to modulate cognitive flexibility as assessed by performance on anagrams. To determine whether these findings on noradrenergic modulation of cognitive flexibility are specific to the noradrenergic system or are a nonspecific anxiety effect, we compared the effects of propranolol, lorazepam, and placebo on the anagram task. Subjects attended 3 test sessions. Prior to each session, subjects were given 1 of the 3 drugs. As in previous research, the natural log of the solution latency of each test item was summed for each test session and compared across drug conditions. For subjects able to solve the anagrams, solution times after propranolol, but not lorazepam, were significantly lower than after placebo. Therefore, this suggests that the phenomenon of noradrenergic modulation of cognitive flexibility does not result from a nonspecific anxiolytic effect, but rather is specific to the noradrenergic system.

Phytoremediation potential of transplanted bare-root seedlings of trees for lead/zinc and copper mine tailings.

PubMed

Shi, Xiang; Chen, Yi-Tai; Wang, Shu-Feng; Pan, Hong-Wei; Sun, Hai-Jing; Liu, Cai-Xia; Liu, Jian-Feng; Jiang, Ze-Ping

2016-11-01

Selecting plant species that can overcome unfavorable conditions and increase the recovery of degraded mined lands remains a challenge. A pot experiment was conducted to evaluate the feasibility of using transplanted tree seedlings for the phytoremediation of lead/zinc and copper mine tailings. One-year-old bare-root of woody species (Rhus chinensis Mill, Quercus acutissima Carruth, Liquidambar formosana Hance, Vitex trifolia Linn. var. simplicifolia Cham, Lespedeza cuneata and Amorpha fruticosa Linn) were transplanted into pots with mine tailings and tested as potential metal-tolerant plants. Seedling survival, plant growth, root trait, nutrient uptake, and metal accumulation and translocation were assessed. The six species grew in both tailings and showed different tolerance level. A. fruticosa was highly tolerant of Zn, Pb and Cu, and grew normally in both tailings. Metal concentrations were higher in the roots than in the shoots of the six species. All of the species had low bioconcentration and translocation factor values. However, R. chinensis and L. formosana had significantly higher translocation factor values for Pb (0.88) and Zn (1.78) than the other species. The nitrogen-fixing species, A. fruticosa, had the highest tolerance and biomass production, implying that it has great potential in the phytoremediation of tailing areas in southern China.

Flexible Blades for Wind Turbines

NASA Astrophysics Data System (ADS)

Collins, Madeline Carlisle; Macphee, David; Harris, Caleb

2016-11-01

Previous research has shown that windmills with flexible blades are more efficient than those with rigid blades. Flexibility offers passive pitch control, preferable to active pitch control which is costly and requires maintenance. Flexible blades morph such that the blade more closely resembles its design point at part load and over load. The lift-to-drag ratios on individual blades was investigated. A mold was designed and machined from an acrylic slab for the casting of blades with a NACA 0012 cross section. A flexible blade was cast from silicone and a rigid blade was cast from polyurethane. Each of these blades was tested in a wind tunnel, cantilever mounted, spanning the whole test section. The angle of attack was varied by rotating the mount. All tests were performed at the same wind speed. A load cell within the mount measured forces on the blade, from which the lift and drag forces were calculated. The stall point for the flexible blade occurred later than for the rigid blade, which agrees with previous research. Lift-to-drag ratios were larger for the flexible blade at all angles of attack tested. Flexible blades seem to be a viable option for passive pitch control. Future research will include different airfoil cross sections, wind speeds, and blade materials. Funding from NSF REU site Grant EEC 1358991 is greatly appreciated.

The deep-tow marine controlled-source electromagnetic transmitter system for gas hydrate exploration

NASA Astrophysics Data System (ADS)

Wang, Meng; Deng, Ming; Wu, Zhongliang; Luo, Xianhu; Jing, Jianen; Chen, Kai

2017-02-01

The Marine Controlled-Source Electromagnetic (MCSEM) method has been recognized as an important and effective tool to detect electrically resistive structures, such as oil, gas, and gas hydrate. The MCSEM performance is strongly influenced by the transmitter system design. We have developed a deep-tow MCSEM transmitter system. In this paper, some new technical details will be present. A 10,000 m optical-electrical composite cable is used to support high power transmission and fast data transfer; a new clock unit is designed to keep the synchronization between transmitter and receivers, and mark the time stamp into the transmission current full waveform; a data link is established to monitor the real-time altitude of the tail unit; an online insulation measuring instrument is adopted to monitor current leakage from high voltage transformer; a neutrally buoyant dipole antenna of copper cable and flexible electrodes are created to transmit the large power current into seawater; a new design method for the transmitter, which is called "real-time control technology of hardware parallelism", is described to achieve inverting and recording high-power current waveform, controlling functions, and collecting auxiliary information. We use a gas hydrate exploration test to verify the performance of the transmitter system, focusing on more technical details, rather than applications. The test shows that the transmitter can be used for gas hydrate exploration as an effective source.

Bed-Load Dispersion: A Literature Review

DTIC Science & Technology

2016-12-01

buried. The observed mean and variance of particle dis- placements from experimental measurements at specific time snap-shots can be used to determine...dispersion coefficient equation: . ( 8 ) For the range of experimental conditions tested within the Chang and Yen (2002) study, their equation... surveys , 51 exhibited thin-tail distri- butions and 8 more could have been considered thin-tail based on the definition of the ‘tail’. Liebault et al

Cognitive flexibility and religious disbelief.

PubMed

Zmigrod, Leor; Rentfrow, P Jason; Zmigrod, Sharon; Robbins, Trevor W

2018-06-11

Cognitive flexibility is operationalized in the neuropsychological literature as the ability to shift between modes of thinking and adapt to novel or changing environments. Religious belief systems consist of strict rules and rituals that offer adherents certainty, consistency, and stability. Consequently, we hypothesized that religious adherence and practice of repetitive religious rituals may be related to the persistence versus flexibility of one's cognition. The present study investigated the extent to which tendencies towards cognitive flexibility versus persistence are related to three facets of religious life: religious affiliation, religious practice, and religious upbringing. In a large sample (N = 744), we found that religious disbelief was related to cognitive flexibility across three independent behavioural measures: the Wisconsin Card Sorting Test, Remote Associates Test, and Alternative Uses Test. Furthermore, lower frequency of religious service attendance was related to cognitive flexibility. When analysing participants' religious upbringing in relation to their current religious affiliation, it was manifest that current affiliation was more influential than religious upbringing in all the measured facets of cognitive flexibility. The findings indicate that religious affiliation and engagement may shape and be shaped by cognitive control styles towards flexibility versus persistence, highlighting the tight links between flexibility of thought and religious ideologies.

Modulation by alpha-difluoromethyl-ornithine and aminoguanidine of pain threshold, morphine analgesia and tolerance.

PubMed

Lu, Gang; Su, Rui-Bin; Li, Jin; Qin, Bo-Yi

2003-10-08

The effects of alpha-difluoromethyl-ornithine (DFMO) and aminoguanidine, which might influence the metabolism of endogenous agmatine, on pain threshold, morphine analgesia and tolerance were investigated in mice. In the mouse acetic acid writhing test, intracerebroventricular (i.c.v.) injection of DFMO or aminoguanidine significantly elevated the pain threshold as indicated by a decrease in the number of writhings. DFMO or aminoguanidine obviously increased the analgesic effect of morphine in the mouse acetic acid writhing test and the mouse heat radiation tail-flick assay. These effects of DFMO and aminoguanidine were antagonized by idazoxan (3 mg/kg, i.p.), which is a selective antagonist of the imidazoline receptor. In the mouse heat radiation tail-flick assay, aminoguanidine significantly prolonged the tail-flick latency of animals, suggesting that the pain threshold was elevated. Furthermore, both DFMO and aminoguanidine enhanced morphine analgesia and inhibited acute morphine tolerance in the mouse heat radiation tail-flick assay. Neither DFMO nor aminoguanidine inhibited the activity of nitric oxide synthase in different brain areas in mice in vivo. These results indicate that the substances involved in the metabolism of endogenous agmatine could modulate the pain threshold, morphine analgesia and tolerance, indicating the possible role of endogenous agmatine in the pharmacological effects of morphine.

Molecular Design of Branched and Binary Molecules at Ordered Interfaces

DOE Office of Scientific and Technical Information (OSTI.GOV)

Genson, Kirsten Larson

2005-01-01

This study examined five different branched molecular architectures to discern the effect of design on the ability of molecules to form ordered structures at interfaces. Photochromic monodendrons formed kinked packing structures at the air-water interface due to the cross-sectional area mismatch created by varying number of alkyl tails and the hydrophilic polar head group. The lower generations formed orthorhombic unit cell with long range ordering despite the alkyl tails tilted to a large degree. Favorable interactions between liquid crystalline terminal groups and the underlying substrate were observed to compel a flexible carbosilane dendrimer core to form a compressed elliptical conformationmore » which packed stagger within lamellae domains with limited short range ordering. A twelve arm binary star polymer was observed to form two dimensional micelles at the air-water interface attributed to the higher polystyrene block composition. Linear rod-coil molecules formed a multitude of packing structures at the air-water interface due to the varying composition. Tree-like rod-coil molecules demonstrated the ability to form one-dimensional structures at the air-water interface and at the air-solvent interface caused by the preferential ordering of the rigid rod cores. The role of molecular architecture and composition was examined and the influence chemically competing fragments was shown to exert on the packing structure. The amphiphilic balance of the different molecular series exhibited control on the ordering behavior at the air-water interface and within bulk structures. The shell nature and tail type was determined to dictate the preferential ordering structure and molecular reorganization at interfaces with the core nature effect secondary.« less

Hydrophobic hydration and the anomalous partial molar volumes in ethanol-water mixtures

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tan, Ming-Liang; Te, Jerez; Cendagorta, Joseph R.

2015-02-14

The anomalous behavior in the partial molar volumes of ethanol-water mixtures at low concentrations of ethanol is studied using molecular dynamics simulations. Previous work indicates that the striking minimum in the partial molar volume of ethanol V{sub E} as a function of ethanol mole fraction X{sub E} is determined mainly by water-water interactions. These results were based on simulations that used one water model for the solute-water interactions but two different water models for the water-water interactions. This is confirmed here by using two more water models for the water-water interactions. Furthermore, the previous work indicates that the initial decreasemore » is caused by association of the hydration shells of the hydrocarbon tails, and the minimum occurs at the concentration where all of the hydration shells are touching each other. Thus, the characteristics of the hydration of the tail that cause the decrease and the features of the water models that reproduce this type of hydration are also examined here. The results show that a single-site multipole water model with a charge distribution that mimics the large quadrupole and the p-orbital type electron density out of the molecular plane has “brittle” hydration with hydrogen bonds that break as the tails touch, which reproduces the deep minimum. However, water models with more typical site representations with partial charges lead to flexible hydration that tends to stay intact, which produces a shallow minimum. Thus, brittle hydration may play an essential role in hydrophobic association in water.« less

Geochemical behavior and environmental risks related to the use of abandoned base-metal tailings as construction material in the upper-Moulouya district, Morocco.

PubMed

Argane, R; El Adnani, M; Benzaazoua, M; Bouzahzah, H; Khalil, A; Hakkou, R; Taha, Y

2016-01-01

In some developing countries, base-metal residues that were abandoned in tailing ponds or impoundments are increasingly used as construction material without any control, engineering basis, or environmental concern. This uncontrolled reuse of mine tailings may constitute a new form of pollution risks for humans and ecosystems through metal leaching. Therefore, the aim of the current study is to assess mine drainage, metal mobility, and geochemical behavior of two abandoned mine tailings commonly used in the upper-Moulouya region (eastern Morocco) as fine aggregates for mortar preparation. Their detailed physical, chemical, and mineralogical properties were subsequently evaluated in the context of developing appropriate alternative reuses to replace their conventional disposal and limit their weathering exposure. The obtained results showed that both tailings contain relatively high quantities of residual metals and metalloids with lead (ranging between 3610 and 5940 mg/kg) being the major pollutant. However, the mineralogical investigations revealed the presence of abundant neutralizing minerals and low sulfide content which influence mine drainage geochemistry and subsequently lower metals mobility. In fact, leachate analyses from weathering cell kinetic tests showed neutral conditions and low sulfide oxidation rates. According to these results, the tailings used as construction material in the upper-Moulouya region have very low generating potential of contaminated effluents and their reuse as aggregates may constitute a sustainable alternative method for efficient tailing management.

Giant surfactants of poly(ethylene oxide)- b-polystyrene-(molecular nanoparticle): nanoparticle-driven self-assembly with sub-10-nm nanostructures in thin films

NASA Astrophysics Data System (ADS)

Hsu, Chih-Hao; Lin, Zhiwei; Dong, Xue-Hui; Hsieh, I.-Fan; Cheng, Stephen Z. D.

2014-03-01

Giant surfactants are built upon precisely attaching shape- and volume-persistent molecular nanoparticles (MNP) to polymeric flexible tails. The unique class of self-assembling materials, giant surfactants, has been demonstrated to form self-assembled ordered nanostructures, and their self-assembly behaviors are remarkably sensitive to primary chemical structures. In this work, two sets of giant surfactants with functionalized MNP attached to diblock copolymer tails were studied in thin films. Carboxylic acid-functionalized [60]fullerene (AC60) tethered with PEO- b-PS (PEO-PS-AC60) represents an ABA' (hydrophilic-hydrophobic-hydrophilic) giant surfactant, and fluoro-functionalized polyhedral oligomeric silsesquioxane (FPOSS) tethered with PEO- b-PS (PEO-PS-FPOSS) represents an ABC (hydrophilic-hydrophobic-omniphobic) one. The dissimilar chemical natures of the MNPs result in different arrangement of MNPs in self-assembled structures, the dispersion of AC60 in PEO domain and the single domain of FPOSS. Moreover, the chemically bonded MNPs could induce the originally disordered small molecular PEO- b-PS to form ordered cylindrical and lamellar structure, as evidenced by TEM and GISAXS, leading to sub-10-nm nanostructures of copolymer in the thin film state.

Chromatin ionic atmosphere analyzed by a mesoscale electrostatic approach.

PubMed

Gan, Hin Hark; Schlick, Tamar

2010-10-20

Characterizing the ionic distribution around chromatin is important for understanding the electrostatic forces governing chromatin structure and function. Here we develop an electrostatic model to handle multivalent ions and compute the ionic distribution around a mesoscale chromatin model as a function of conformation, number of nucleosome cores, and ionic strength and species using Poisson-Boltzmann theory. This approach enables us to visualize and measure the complex patterns of counterion condensation around chromatin by examining ionic densities, free energies, shielding charges, and correlations of shielding charges around the nucleosome core and various oligonucleosome conformations. We show that: counterions, especially divalent cations, predominantly condense around the nucleosomal and linker DNA, unburied regions of histone tails, and exposed chromatin surfaces; ionic screening is sensitively influenced by local and global conformations, with a wide ranging net nucleosome core screening charge (56-100e); and screening charge correlations reveal conformational flexibility and interactions among chromatin subunits, especially between the histone tails and parental nucleosome cores. These results provide complementary and detailed views of ionic effects on chromatin structure for modest computational resources. The electrostatic model developed here is applicable to other coarse-grained macromolecular complexes. Copyright © 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.

Structures and physiological roles of 13 integral lipids of bovine heart cytochrome c oxidase

PubMed Central

Shinzawa-Itoh, Kyoko; Aoyama, Hiroshi; Muramoto, Kazumasa; Terada, Hirohito; Kurauchi, Tsuyoshi; Tadehara, Yoshiki; Yamasaki, Akiko; Sugimura, Takashi; Kurono, Sadamu; Tsujimoto, Kazuo; Mizushima, Tsunehiro; Yamashita, Eiki; Tsukihara, Tomitake; Yoshikawa, Shinya

2007-01-01

All 13 lipids, including two cardiolipins, one phosphatidylcholine, three phosphatidylethanolamines, four phosphatidylglycerols and three triglycerides, were identified in a crystalline bovine heart cytochrome c oxidase (CcO) preparation. The chain lengths and unsaturated bond positions of the fatty acid moieties determined by mass spectrometry suggest that each lipid head group identifies its specific binding site within CcOs. The X-ray structure demonstrates that the flexibility of the fatty acid tails facilitates their effective space-filling functions and that the four phospholipids stabilize the CcO dimer. Binding of dicyclohexylcarbodiimide to the O2 transfer pathway of CcO causes two palmitate tails of phosphatidylglycerols to block the pathway, suggesting that the palmitates control the O2 transfer process.The phosphatidylglycerol with vaccenate (cis-Δ11-octadecenoate) was found in CcOs of bovine and Paracoccus denitrificans, the ancestor of mitochondrion, indicating that the vaccenate is conserved in bovine CcO in spite of the abundance of oleate (cis-Δ9-octadecenoate). The X-ray structure indicates that the protein moiety selects cis-vaccenate near the O2 transfer pathway against trans-vaccenate. These results suggest that vaccenate plays a critical role in the O2 transfer mechanism. PMID:17332748

Postnatal development under conditions of simulated weightlessness and space flight

NASA Technical Reports Server (NTRS)

Walton, K.

1998-01-01

The adaptability of the developing nervous system to environmental influences and the mechanisms underlying this plasticity has recently become a subject of interest in space neuroscience. Ground studies on neonatal rats using the tail suspension model of weightlessness have shown that the force of gravity clearly influences the events underlying the postnatal development of motor function. These effects depend on the age of the animal, duration of the perturbation and the motor function studied. A nine-day flight study has shown that a dam and neonates can develop under conditions of space flight. The motor function of the flight animals after landing was consistent with that seen in the tail suspension studies, being marked by limb joint extension. However, there were expected differences due to: (1) the unloading of the vestibular system in flight, which did not occur in the ground-based experiments; (2) differences between flight and suspension durations; and (3) the inability to evaluate motor function during the flight. The next step is to conduct experiments in space with the flexibility and rigor that is now limited to ground studies: an opportunity offered by the International Space Station. Copyright 1998 Published by Elsevier Science B.V.

Herd-level risk factors for bovine viral diarrhea infection in cattle of Tamil Nadu.

PubMed

Kumar, Subbiah Krishna; Palanivel, K M; Sukumar, K; Ronald, B Samuel Masilamoni; Selvaraju, G; Ponnudurai, G

2018-04-01

A cross-sectional study was carried out to identify risk factors for bovine viral diarrhea virus (BVDV) infection in 62 randomly selected dairy herds which were tested for BVD serum antibodies by using an indirect ELISA kit (IDEXX). Results from the chi-square test analysis were interpreted by analyzing by chi-square test. A sum of 500 sera samples were screened and 66 animals (13.20%) showed positive for BVDV antibody. Within herd, BVD seroprevalence was 12-65%. This study concluded that epidemiological risk factors like location, herd size, housing patterns like, tail to tail system, roofing pattern, distance between the manure pit and farm, and distance between farms were significantly associated with BVDV serological status (P < 0.05).

Tail Service Mast Umbilical Arrival

NASA Image and Video Library

2016-08-02

Technicians assist as a crane is used to lift the first Tail Service Mast Umbilical (TSMU) up from the flatbed of the transport truck at the Launch Equipment Test Facility (LETF) at NASA’s Kennedy Space Center in Florida. Two TSMUs will provide liquid propellants and power to the Space Launch System (SLS) rocket’s core stage engine. Both TSMUs will connect to the zero-level deck on the mobile launcher, providing fuel and electricity to the SLS rocket before it launches on Exploration Mission 1. The TSMU will undergo testing and validation at the LETF to verify it is functioning properly. The center’s Engineering Directorate and the Ground Systems Development and Operations Program are overseeing processing and testing of the umbilicals.

Tail Service Mast Umbilical Arrival

NASA Image and Video Library

2016-08-02

Technicians assist as a crane is used to lift the first Tail Service Mast Umbilical (TSMU) away from the flatbed of the transport truck at the Launch Equipment Test Facility (LETF) at NASA’s Kennedy Space Center in Florida. Two TSMUs will provide liquid propellants and power to the Space Launch System (SLS) rocket’s core stage engine. Both TSMUs will connect to the zero-level deck on the mobile launcher, providing fuel and electricity to the SLS rocket before it launches on Exploration Mission 1. The TSMU will undergo testing and validation at the LETF to verify it is functioning properly. The center’s Engineering Directorate and the Ground Systems Development and Operations Program are overseeing processing and testing of the umbilicals.

Mechanics of biomimetic systems propelled by actin comet tails

NASA Astrophysics Data System (ADS)

Kang, Hyeran; Tambe, Dhananjay; Shenoy, Vivek; Tang, Jay

2009-03-01

The motility of intracellular bacterial pathogens such as Listeria monocytogenes is driven by filamentous actin comet tails in a variety of trajectories. Here, we present the in vitro study on the actin-based movements using spherical beads of different sizes coated with VCA protein, a partial domain of N-Wasp, in platelet extracts. Long term two-dimensional trajectories of the spherical beads motility show characteristic difference than those observed for bacteria, which have both elongated shape and asymmetric expression of the polymerization inducing enzyme. The trajectories also vary sensitively with the bead size and shape. These results provide a useful test to our new analytical model including the rotation of the bead relative to the tail.

Tabulated Pressure Coefficient Data from a Tail Loads Investigation on a 1/15-Scale Model of the Goodyear XZP5K Airship

NASA Technical Reports Server (NTRS)

Cannon, Michael D.

1956-01-01

This paper contains tail and hull loads data obtained in an investigation of a l/15-scale model of the Goodyear XZP5K airship. Data are presented in the form of tabulated pressure coefficients over a pitch and yaw range of +/-20 deg and 0 deg to 30 deg respectively, with various rudder and elevator deflections. Two tail configurations of different plan forms were tested on the model. The investigation was conducted in the Langley full-scale tunnel at a Reynolds number of approximately 16.5 x 10(exp 6) based on hull length, which corresponds to a Mach number of about 0.12.

Powassan virus in Ixodes cookei and Mustelidae in New England.

PubMed

Main, A J; Carey, A B; Downs, W G

1979-10-01

Powassan virus was recovered from a pool of 3 nymphal and 1 adult female Ixodes cookei removed from a striped skunk (Mephitis mephitis) trapped in Massachusetts during 1967 and from a pool of 9 nymphal I. cookei from a long-tailed weasel (Mustela frenata) captured in Connecticut during 1978. Virus was detected in the blood of both mammals. Hemagglutination-inhibiting (HI) antibody to Powassan virus was demonstrated in 16.0% of the skunks sampled in Connecticut, and neutralizing antibody was detected in 83.3% of the skunks tested from Massachusetts. HI antibody was found in 1 of 6 long-tailed weasels from Connecticut and 1 of 6 short-tailed weasels (Mustela erminea) from Maine.

Interference of Tail Surfaces and Wing and Fuselage from Tests of 17 Combinations in the N.A.C.A. Variable-Density Tunnel

NASA Technical Reports Server (NTRS)

Sherman, Albert

1939-01-01

An investigation of the interference associated with tail surfaces added to wing-fuselage combinations was included in the interference program in progress in the NACA variable-density tunnel. The results indicate that, in aerodynamically clean combinations, the increment to the high-speed drag can be estimated from section characteristics within useful limits of accuracy. The interference appears mainly as effects on the downwash angel and as losses in the tail. An interference burble, which markedly increases the glide-path angle and the stability in pitch before the actual stall, may be considered a means of obtaining satisfactory stalling characteristics for a complete combination.

Descriptive Epidemiology and Whole Genome Sequencing Analysis for an Outbreak of Bovine Tuberculosis in Beef Cattle and White-Tailed Deer in Northwestern Minnesota.

PubMed

Glaser, Linda; Carstensen, Michelle; Shaw, Sheryl; Robbe-Austerman, Suelee; Wunschmann, Arno; Grear, Dan; Stuber, Tod; Thomsen, Bruce

2016-01-01

Bovine tuberculosis (bTB) was discovered in a Minnesota cow through routine slaughter surveillance in 2005 and the resulting epidemiological investigation led to the discovery of infection in both cattle and white-tailed deer in the state. From 2005 through 2009, a total of 12 beef cattle herds and 27 free-ranging white-tailed deer (Odocoileus virginianus) were found infected in a small geographic region of northwestern Minnesota. Genotyping of isolates determined both cattle and deer shared the same strain of bTB, and it was similar to types found in cattle in the southwestern United States and Mexico. Whole genomic sequencing confirmed the introduction of this infection into Minnesota was recent, with little genetic divergence. Aggressive surveillance and management efforts in both cattle and deer continued from 2010-2012; no additional infections were discovered. Over 10,000 deer were tested and 705 whole herd cattle tests performed in the investigation of this outbreak.

Bioadhesive films containing benzocaine: correlation between in vitro permeation and in vivo local anesthetic effect.

PubMed

de Araujo, Daniele Ribeiro; Padula, Cristina; Cereda, Cíntia Maria Saia; Tófoli, Giovana Radomille; Brito, Rui Barbosa; de Paula, Eneida; Nicoli, Sara; Santi, Patrizia

2010-08-01

The aim of this work was to develop anesthetic bioadhesive films containing benzocaine and study their in vitro skin permeation and in vivo performance, in comparison with commercial formulations. Films containing 3% and 5% w/w of benzocaine were prepared and characterized by weight, drug content, thickness and morphology. In vitro permeation assays were performed in vertical diffusion cells using full-thickness pig ear skin as barrier. Intensity and duration of analgesia were evaluated in rats by tail-flick test, and skin histological analysis was carried out. Tail-flick test showed that the duration of benzocaine-induced analgesia was significantly prolonged with the films compared to commercial creams, in agreement with the higher in vitro permeation. Histological analysis of the rat tail skin did not reveal morphological tissue changes nor cell infiltration signs after application of the commercial creams or films. Results from our study indicate that the films developed in this work can be considered as innovative dermal/transdermal therapeutic systems for benzocaine local delivery.

Descriptive Epidemiology and Whole Genome Sequencing Analysis for an Outbreak of Bovine Tuberculosis in Beef Cattle and White-Tailed Deer in Northwestern Minnesota

PubMed Central

Glaser, Linda; Carstensen, Michelle; Shaw, Sheryl; Robbe-Austerman, Suelee; Wunschmann, Arno; Grear, Dan; Stuber, Tod; Thomsen, Bruce

2016-01-01

Bovine tuberculosis (bTB) was discovered in a Minnesota cow through routine slaughter surveillance in 2005 and the resulting epidemiological investigation led to the discovery of infection in both cattle and white-tailed deer in the state. From 2005 through 2009, a total of 12 beef cattle herds and 27 free-ranging white-tailed deer (Odocoileus virginianus) were found infected in a small geographic region of northwestern Minnesota. Genotyping of isolates determined both cattle and deer shared the same strain of bTB, and it was similar to types found in cattle in the southwestern United States and Mexico. Whole genomic sequencing confirmed the introduction of this infection into Minnesota was recent, with little genetic divergence. Aggressive surveillance and management efforts in both cattle and deer continued from 2010–2012; no additional infections were discovered. Over 10,000 deer were tested and 705 whole herd cattle tests performed in the investigation of this outbreak. PMID:26785113

Wind-Tunnel Investigation at Low Speed of the Effects of Chordwise Wing Fences and Horizontal-Tail Position on the Static Longitudinal Stability Characteristics of an Airplane Model with a 35 Degree Sweptback Wing

NASA Technical Reports Server (NTRS)

Queijo, M J; Jaquet, Byron M; Wolhart, Walter D

1954-01-01

Low-speed tests of a model with a wing swept back 35 degrees at the 0.33-chord line and a horizontal tail located well above the extended wing-chord plane indicated static longitudinal instability at moderate angles of attack for all configurations tested. An investigation therefore was made to determine whether the longitudinal stability could be improved by the use of chordwise wing fences, by lowering the horizontal tail, or by a combination of both. The results of the investigation showed that the longitudinal stability characteristics of the model with slats retracted could be improved at moderate angles of attack by placing chordwise wing fences at a spanwise station of about 73 percent of the wing semispan from the plane of symmetry provided the nose of the fence extended slightly beyond or around the wing leading edge.

Antimicrobial Susceptibility Testing of Mycobacterium bovis Isolates from Michigan White-Tailed Deer during the 2009 Hunting Season.

PubMed

Fitzgerald, Scott D; Schooley, Angie M; Berry, Dale E; Kaneene, John B

2010-12-02

Michigan has had an ongoing outbreak of endemic Mycobacterium bovis which has been recognized within and sustained by its free-ranging white-tailed deer population since 1994. Worldwide, organisms within the Mycobacterium tuberculosis complex have exhibited the ability to develop resistance to antimicrobial agents, resulting in both the multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains of human tuberculosis. Michigan's Bovine Tuberculosis Working Group has conducted active antimicrobial susceptibility testing on wildlife isolates of the endemic M. bovis organism at five-year intervals to detect any emerging drug resistance patterns. The results of 33 white-tailed deer origin isolates collected from the 2009 hunting season are reported here. There continues to be no evidence of any drug resistance except for pyrazinamide resistance. These results are likely due to the lack of antibacterial treatment applied to either wildlife or domestic animals which would provide selection pressure for the development of drug resistance.

49 CFR 178.1050 - Top lift test.

Code of Federal Regulations, 2014 CFR

2014-10-01

... designed to be lifted from the top, there may be no damage to the Flexible Bulk Container or its lifting devices that renders the Flexible Bulk Container unsafe for transport, and no loss of contents. ... permissible gross mass, the load being evenly distributed. (c) Test method. (1) A Flexible Bulk Container must...

49 CFR 178.1050 - Top lift test.

Code of Federal Regulations, 2013 CFR

2013-10-01

... designed to be lifted from the top, there may be no damage to the Flexible Bulk Container or its lifting devices that renders the Flexible Bulk Container unsafe for transport, and no loss of contents. ... permissible gross mass, the load being evenly distributed. (c) Test method. (1) A Flexible Bulk Container must...

Experimental and analytical transonic flutter characteristics of a geared-elevator configuration

NASA Technical Reports Server (NTRS)

Ruhlin, C. L.; Doggett, R. V., Jr.; Gregory, R. A.

1980-01-01

The flutter model represented the aft fuselage and empennage of a proposed supersonic transport airplane and had an all movable horizontal tail with a geared elevator. It was tested mounted from a sting in the transonic dynamics tunnel. Symmetric flutter boundaries were determined experimentally at Mach numbers from 0.7 to 1.14 for a geared elevator configuration (gear ratio of 2.8 to 1.0) and an ungeared elevator configuration (gear ratio of 1.0 to 1.0). Gearing the elevator increased the experimental flutter dynamic pressures about 20 percent. Flutter calculations were made for the geared elevator configuration by using two analytical methods based on subsonic lifting surface theory. Both methods analyzed the stabilizer and elevator as a single, deforming surface, but one method also allowed the elevator to be analyzed as hinged from the stabilizer. All analyses predicted lower flutter dynamic pressures than experiment with best agreement (within 12 percent) for the hinged elevator method. Considering the model as mounted from a flexible rather than rigid sting in the analyses, had only a slight effect on the flutter results but was significant in that a sting related vibration mode was identified as a potentially flutter critical mode.

Synthesis and structure-activity relationship of novel cinnamamide derivatives as antidepressant agents.

PubMed

Han, Min; Ma, Xiaohui; Jin, Yuanpeng; Zhou, Wangyi; Cao, Jing; Wang, Yahu; Zhou, Shuiping; Wang, Guocheng; Zhu, Yonghong

2014-11-15

Cinnamamide 3a, a leading compound with antidepressant-like activity, and its derivatives were synthesized and their antidepressant activity and structure-activity relationship were investigated. Most of the compounds with trifluoromethyl group in methylenedioxyphenyl moiety (3f, 4b-c and 6a-b) exhibited significant antidepressant activity, measured in terms of percentage decrease in immobility duration by tail suspension test. In addition, the dose-dependent antidepressant effect of the most potent compound 3f was subsequently confirmed in tail suspension test and forced swim test. The test results showed that 3f was equal to or more effective than the standard drug fluoxetine at a concentration of 10mg/kg. Furthermore, compound 3f did not show any central nervous system stimulant properties in the open-field test and the preliminary results were promising enough to warrant further detailed antidepressant research around this scaffold. Copyright © 2014 Elsevier Ltd. All rights reserved.

Assessment of spinal flexibility in adolescent idiopathic scoliosis: suspension versus side-bending radiography.

PubMed

Lamarre, Marie-Eve; Parent, Stefan; Labelle, Hubert; Aubin, Carl-Eric; Joncas, Julie; Cabral, Anne; Petit, Yvan

2009-03-15

Prospective evaluation of a new suspension test to determine curve flexibility in adolescent idiopathic scoliosis (AIS) in comparison with erect side-bending. To verify whether the suspension is a better method than side-bending to estimate curve reducibility and to assess spine flexibility. Spinal flexibility is a decisive biomechanical parameter for the planning of AIS surgery. Side-bending is often referred as the gold standard, but it has a low reproducibility and there is no agreement amongst surgeons about the most advantageous method to use. Even more, every technique evaluates reducibility instead of flexibility since the forces involved in the change in shape of the spine are not considered. Eighteen patients scheduled for AIS surgery were studied. Preoperative radiological evaluation consisted of 4 radiographs: standing posteroanterior, left and right erect side-bending, and suspension. The side-bending and the suspension tests were compared on the basis of the apical vertebrae derotation and the scoliosis curve reduction. Frontal and axial flexibility indices, expressed as the ratio between the moment induced by the body weight and the reduction, were calculated from the suspension data. The average scoliosis curve reduction and apical vertebra derotation were 21 degrees (37%) and 3 degrees (12%), respectively for erect side-bending and 26 degrees (39%) and 7 degrees (28%), respectively for suspension. The erect side-bending test generated a larger curve reduction (P = 0.05) when considering the moderate curves only and the suspension test (P = 0.02) when considering the severe curves. The suspension test produced a larger axial derotation (P = 0.007) when considering all the curves. The average traction force during suspension was 306 N (187 N-377 N). The average estimation for the frontal flexibility index was 1.64 degrees/Nm (0.84-2.82) and 0.51 degrees/Nm (0.01-1.39) for the axial flexibility index. Results of this study demonstrate the feasibility to really evaluate the spine flexibility with the suspension test. The estimated flexibility values are realistic and similar to those reported in vitro. Suspension should be used in the future for spine flexibility assessment.

Determination of the criterion-related validity of hip joint angle test for estimating hamstring flexibility using a contemporary statistical approach.

PubMed

Sainz de Baranda, Pilar; Rodríguez-Iniesta, María; Ayala, Francisco; Santonja, Fernando; Cejudo, Antonio

2014-07-01

To examine the criterion-related validity of the horizontal hip joint angle (H-HJA) test and vertical hip joint angle (V-HJA) test for estimating hamstring flexibility measured through the passive straight-leg raise (PSLR) test using contemporary statistical measures. Validity study. Controlled laboratory environment. One hundred thirty-eight professional trampoline gymnasts (61 women and 77 men). Hamstring flexibility. Each participant performed 2 trials of H-HJA, V-HJA, and PSLR tests in a randomized order. The criterion-related validity of H-HJA and V-HJA tests was measured through the estimation equation, typical error of the estimate (TEEST), validity correlation (β), and their respective confidence limits. The findings from this study suggest that although H-HJA and V-HJA tests showed moderate to high validity scores for estimating hamstring flexibility (standardized TEEST = 0.63; β = 0.80), the TEEST statistic reported for both tests was not narrow enough for clinical purposes (H-HJA = 10.3 degrees; V-HJA = 9.5 degrees). Subsequently, the predicted likely thresholds for the true values that were generated were too wide (H-HJA = predicted value ± 13.2 degrees; V-HJA = predicted value ± 12.2 degrees). The results suggest that although the HJA test showed moderate to high validity scores for estimating hamstring flexibility, the prediction intervals between the HJA and PSLR tests are not strong enough to suggest that clinicians and sport medicine practitioners should use the HJA and PSLR tests interchangeably as gold standard measurement tools to evaluate and detect short hamstring muscle flexibility.

Predictable hotspots and foraging habitat of the endangered short-tailed albatross (Phoebastria albatrus) in the North Pacific: Implications for conservation

USGS Publications Warehouse

Piatt, John F.; Wetzel, J.; Bell, K.; DeGange, A.R.; Balogh, G.R.; Drew, G.S.; Geernaert, T.; Ladd, C.; Byrd, G.V.

2006-01-01

The short-tailed albatross (Phoebastria albatrus) is a rare and endangered seabird that ranges widely over the northern North Pacific. Populations are slowly recovering but birds face several threats at sea, in particular the incidental capture of birds in long-line fisheries. Conservation efforts are hampered by a lack of information about the at-sea distribution of this species, especially knowledge of where it may predictably co-occur with long-line fishing effort. During 18 years of transiting the Aleutian Islands Unit of the Alaska Maritime National Wildlife Refuge on a research vessel, we observed short-tailed albatross on 65 occasions. They were consistently observed near Ingenstrem Rocks (Buldir Pass) in the western Aleutians and near Seguam Pass in the central Aleutians. Based on the oceanographic characteristics of the locations where we saw most of the birds, we hypothesized that short-tailed albatross “hotspots” were located where tidal currents and steep bottom topography generate strong vertical mixing along the Aleutian Archipelago. As a test of this hypothesis, we analyzed a database containing 1432 opportunistic observations of 2463 short-tailed albatross at sea in the North Pacific. These data showed that short-tailed albatross were closely associated with shelf-edge habitats throughout the northern Gulf of Alaska and Bering Sea. In addition to Ingenstrem Rocks and Seguam Pass, important hotspots for short-tailed albatross in the Aleutians included Near Strait, Samalga Pass, and the shelf-edge south of Umnak/Unalaska islands. In the Bering Sea, hotspots were located along margins of Zhemchug, St. Matthews and Pervenets canyons. Because these short-tailed albatross hotspots are predictable, they are also protectable by regulation of threatening activities at local spatial scales.

Simulated predator stimuli reduce brain cell proliferation in two electric fish species, Brachyhypopomus gauderio and Apteronotus leptorhynchus.

PubMed

Dunlap, Kent D; Keane, Geoffrey; Ragazzi, Michael; Lasky, Elise; Salazar, Vielka L

2017-07-01

The brain structure of many animals is influenced by their predators, but the cellular processes underlying this brain plasticity are not well understood. Previous studies showed that electric fish ( Brachyhypopomus occidentalis ) naturally exposed to high predator ( Rhamdia quelen ) density and tail injury had reduced brain cell proliferation compared with individuals facing few predators and those with intact tails. However, these field studies described only correlations between predator exposure and cell proliferation. Here, we used a congener Brachyhypopomus gauderio and another electric fish Apteronotus leptorhynchus to experimentally test the hypothesis that exposure to a predator stimulus and tail injury causes alterations in brain cell proliferation. To simulate predator exposure, we either amputated the tail followed by short-term (1 day) or long-term (17-18 days) recovery or repeatedly chased intact fish with a plastic rod over a 7 day period. We measured cell proliferation (PCNA+ cell density) in the telencephalon and diencephalon, and plasma cortisol, which commonly mediates stress-induced changes in brain cell proliferation. In both species, either tail amputation or simulated predator chase decreased cell proliferation in the telencephalon in a manner resembling the effect of predators in the field. In A. leptorhynchus , cell proliferation decreased drastically in the short term after tail amputation and partially rebounded after long-term recovery. In B. gauderio , tail amputation elevated cortisol levels, but repeated chasing had no effect. In A. leptorhynchus , tail amputation elevated cortisol levels in the short term but not in the long term. Thus, predator stimuli can cause reductions in brain cell proliferation, but the role of cortisol is not clear. © 2017. Published by The Company of Biologists Ltd.

Endogenous DNA damage and testicular germ cell tumors

PubMed Central

Cook, Michael B.; Sigurdson, Alice J.; Jones, Irene M.; Thomas, Cynthia B.; Graubard, Barry I.; Korde, Larissa; Greene, Mark H.; McGlynn, Katherine A.

2008-01-01

Testicular germ cell tumors (TGCT) are comprised of two histologic groups, seminomas and nonseminomas. We postulated that the possible divergent pathogeneses of these histologies may be partially explained by variable levels of net endogenous DNA damage. To test our hypothesis, we conducted a case-case analysis of 51 seminoma and 61 nonseminoma patients using data and specimens from the Familial Testicular Cancer study and the U.S. Radiologic Technologists cohort. A lymphoblastoid cell line was cultured for each patient and the alkaline comet assay was used to determine four parameters: tail DNA, tail length, comet distributed moment (CDM) and Olive tail moment (OTM). Odds ratios (OR) and 95% confidence intervals (95%CI) were estimated using logistic regression. Values for tail length, tail DNA, CDM and OTM were modeled as categorical variables using the 50th and 75th percentiles of the seminoma group. Tail DNA was significantly associated with nonseminoma compared to seminoma (OR50th percentile=3.31, 95%CI: 1.00, 10.98; OR75th percentile=3.71, 95%CI: 1.04, 13.20; p for trend=0.039). OTM exhibited similar, albeit statistically non-significant, risk estimates (OR50th percentile=2.27, 95%CI: 0.75, 6.87; OR75th percentile=2.40, 95%CI: 0.75, 7.71; p for trend=0.12) whereas tail length and CDM showed no association. In conclusion, the results for tail DNA and OTM indicate that net endogenous levels are higher in patients who develop nonseminoma compared with seminoma. This may partly explain the more aggressive biology and younger age-of-onset of this histologic subgroup compared with the relatively less aggressive, later-onset seminoma. PMID:18657195

The minimum alveolar concentration of sevoflurane in ring-tailed lemurs (Lemur catta) and aye-ayes (Daubentonia madagascariensis).

PubMed

Chinnadurai, Sathya K; Williams, Cathy

2016-01-01

To determine the minimum alveolar concentration (MAC) of sevoflurane for ring-tailed lemurs (Lemur catta) and aye-ayes (Daubentonia madagascariensis). Prospective experimental trial. Six adult ring-tailed lemurs, aged 1.3-11.2 years (median age: 8.26) and weighing a mean ± standard deviation (SD) of 2283 ± 254 g. Five adult aye-ayes, aged 4.4-19.3 years (median age: 8.0) and weighing 2712 ± 191 g. Minimum alveolar concentration of sevoflurane was determined using a tail-clamp stimulus. The end-tidal sevoflurane (Fe'Sevo) concentration was increased or decreased by approximately 10% after a positive or negative response to tail clamping, respectively. This procedure was repeated until a positive and negative result were seen on two consecutive trials (i.e. a negative result was achieved and a single 10% decrease in Fe'Sevo concentration resulted in a positive test). The MAC for that animal was determined to be the mean of the concentrations at the two consecutive trials. The mean ± SD MAC of sevoflurane for ring-tailed lemurs was 3.48 ± 0.55% and 1.84 ± 0.17 for aye-ayes. This represents a 47.1% higher MAC in ring-tailed lemurs compared to aye-ayes. The sevoflurane MAC was significantly higher in ring-tailed lemurs, compared to aye-ayes. The MAC of sevoflurane in aye-ayes is consistent with reported MAC values in other species. Extrapolation of sevoflurane anesthetic dose between different species of lemurs could lead to significant errors in anesthetic dosing. © 2015 Association of Veterinary Anaesthetists and the American College of Veterinary Anesthesia and Analgesia.

Underwater thrust and power generation using flexible piezoelectric composites: an experimental investigation toward self-powered swimmer-sensor platforms

NASA Astrophysics Data System (ADS)

Erturk, Alper; Delporte, Ghislain

2011-12-01

Fiber-based flexible piezoelectric composites offer several advantages to use in energy harvesting and biomimetic locomotion. These advantages include ease of application, high power density, effective bending actuation, silent operation over a range of frequencies, and light weight. Piezoelectric materials exhibit the well-known direct and converse piezoelectric effects. The direct piezoelectric effect has received growing attention for low-power generation to use in wireless electronic applications while the converse piezoelectric effect constitutes an alternative to replace the conventional actuators used in biomimetic locomotion. In this paper, underwater thrust and electricity generation are investigated experimentally by focusing on biomimetic structures with macro-fiber composite piezoelectrics. Fish-like bimorph configurations with and without a passive caudal fin (tail) are fabricated and compared. The favorable effect of having a passive caudal fin on the frequency bandwidth is reported. The presence of a passive caudal fin is observed to bring the second bending mode close to the first one, yielding a wideband behavior in thrust generation. The same smart fish configuration is tested for underwater piezoelectric power generation in response to harmonic excitation from its head. Resonant piezohydroelastic actuation is reported to generate milli-newton level hydrodynamic thrust using milli-watt level actuation power input. The average actuation power requirement for generating a mean thrust of 19 mN at 6 Hz using a 10 g piezoelastic fish with a caudal fin is measured as 120 mW. This work also discusses the feasibility of thrust generation using the harvested energy toward enabling self-powered swimmer-sensor platforms with comparisons based on the capacity levels of structural thin-film battery layers as well as harvested solar and vibrational energy.

Experimental Test Rig for Optimal Control of Flexible Space Robotic Arms

DTIC Science & Technology

2016-12-01

was used to refine the test bed design and the experimental workflow. Three concepts incorporated various strategies to design a robust flexible link...used to refine the test bed design and the experimental workflow. Three concepts incorporated various strategies to design a robust flexible link... designed to perform the experimentation . The first and second concepts use traditional elastic springs in varying configurations while a third uses a

Intrathecal clonidine and bupivacaine have synergistic analgesia for acute thermally or inflammatory-induced pain in rats.

PubMed

Nishiyama, Tomoki; Hanaoka, Kazuo

2004-04-01

We investigated the interaction between spinally administered bupivacaine and clonidine using an animal model of acute and inflammatory pain. Rats implanted with lumbar intrathecal catheters were injected intrathecally with saline (control), bupivacaine (1 to 100 microg), or clonidine (0.1 to 3 microg) and tested for their responses to thermal stimulation to the tail (tail flick test) and subcutaneous formalin injection into the hindpaw (formalin test). The effects of the combination of bupivacaine and clonidine on both stimuli were tested by isobolographic analysis. General behavior and motor function were examined as side effects. The 50% effective doses of bupivacaine and clonidine were significantly smaller when combined compared with each single drug in both the tail flick test (2.82 and 0.11 microg versus 7.1 and 0.29 microg, respectively) and phase 1 (0.24 and 0.009 microg versus 5.7 and 0.15 microg) and phase 2 (0.31 and 0.012 microg versus 3.2 and 0.16 microg) of the formalin test. Side effects were decreased by the combination. These results suggest a favorable combination of intrathecal bupivacaine and clonidine in the management of acute and inflammatory pain. The analgesic interaction between intrathecally administered bupivacaine and clonidine was examined during acute thermal and inflammatory-induced pain in rats. The analgesia produced by the combination of these two drugs was synergistic in both acute thermal and inflammatory induced pain, with a decrease in behavioral side effects.

Testing the Wildlink activity-detection system on wolves and white-tailed deer

USGS Publications Warehouse

Kunkel, K.E.; Chapman, R.C.; Mech, L.D.; Gese, E.M.

1991-01-01

We tested the reliability and predictive capabilities of the activity meter in the new Wildlink Data Acquisition and Recapture System by comparing activity counts with concurrent observations of captive wolf (Canis lupus) and free-ranging white-tailed deer (Odocoileus virginianus) activity. The Wildlink system stores activity data in a computer within a radio collar with which a biologist can communicate. Three levels of activity could be detected. The Wildlink system provided greater activity discrimination and was more reliable, adaptable, and efficient and was easier to use than conventional telemetry activity systems. The Wildlink system could be highly useful for determining wildlife energy budgets.

Exploring bird aerodynamics using radio-controlled models.

PubMed

Hoey, Robert G

2010-12-01

A series of radio-controlled glider models was constructed by duplicating the aerodynamic shape of soaring birds (raven, turkey vulture, seagull and pelican). Controlled tests were conducted to determine the level of longitudinal and lateral-directional static stability, and to identify the characteristics that allowed flight without a vertical tail. The use of tail-tilt for controlling small bank-angle changes, as observed in soaring birds, was verified. Subsequent tests, using wing-tip ailerons, inferred that birds use a three-dimensional flow pattern around the wing tip (wing tip vortices) to control adverse yaw and to create a small amount of forward thrust in gliding flight.

Correlation of Fin Buffet Pressures on an F/A-18 with Scaled Wind-Tunnel Measurements

NASA Technical Reports Server (NTRS)

Moses, Robert W.; Shah, Gautam H.

1999-01-01

Buffeting is an aeroelastic phenomenon occurring at high angles of attack that plagues high performance aircraft, especially those with twin vertical tails. Previous wind-tunnel and flight tests were conducted to characterize the buffet loads on the vertical tails by measuring surface pressures, bending moments, and accelerations. Following these tests, buffeting responses were computed using the measured buffet pressures and compared to the measured buffeting responses. The calculated results did not match the measured data because the assumed spatial correlation of the buffet pressures was not correct. A better understanding of the partial (spatial) correlation of the differential buffet pressures on the tail was necessary to improve the buffeting predictions. Several wind-tunnel investigations were conducted for this purpose. When compared, the results of these tests show that the partial correlation scales with flight conditions. One of the remaining questions is whether the wind-tunnel data is consistent with flight data. Presented herein, cross-spectra and coherence functions calculated from pressures that were measured on the High Alpha Research Vehicle indicate that the partial correlation of the buffet pressures in flight agrees with the partial correlation observed in the wind tunnel.

Some Tests and Calculations Pertaining to the Dive Path and to Wing and Tail Loads in the Accident to Eastern Airlines C54B Airplane, NC-88814, Near Bainbridge, Maryland, May 30, 1947

NASA Technical Reports Server (NTRS)

Rhode, Richard V.; Stokke, Allen R.; Rogin, Leo

1947-01-01

Several dive paths were calculated for a C54 airplane starting from level flight at an altitude of 4000 feet and from an initial indicated airspeed of 200 miles per hour. The results show that, within the limits of the possible paths permitted by the evidence of the crash at Bainbridge, the speed of impact would be about 370 miles per hour and the time to crash would be between 12 1/2 and 15 1/2 seconds. Tail load calculations indicate that, with moderate negative acceleration of the airplane, the tail would fail near the end of the dive in a manner consistent in several important respects with the evidence. A number of tests were made of the elevator tab control system to determine whether the tab would move by an amount sufficient to have caused the observed dive if the stored energy in the tab control cable were suddenly released. The results of these tests indicated that the probable tab movement is such as to be capable of causing a dive similar to the one observed at Bainbridge.

Flexibility is associated with motor competence in schoolchildren.

PubMed

Lopes, L; Póvoas, S; Mota, J; Okely, A D; Coelho-E-Silva, M J; Cliff, D P; Lopes, V P; Santos, R

2017-12-01

Available data on the associations between motor competence (MC) and flexibility are limited and result inconclusive. This study aims to examine the relationship between flexibility and MC in children. The sample comprised 596 Portuguese children (47.1% girls) aged 9.7 ± 0.6 years. Motor competence was evaluated with the body coordination test, Körperkoordination Test für Kinder. Cardiorespiratory fitness (20-m shuttle run), muscular strength (curl-up and push-up tests), and flexibility (back-saver sit and reach and trunk-lift tests) were evaluated using the Fitnessgram Test Battery. Z-scores by age and gender for the physical fitness tests were constructed. Analysis of variance and regression analysis were performed. Participants in the healthy zone groups of both flexibility tests exhibited significantly better scores of MC than the participants under the healthy zone (P < 0.001). Back-saver sit and reach and trunk-lift Z-scores, either individually or as a sum, were significant predictors of MC (P < 0.05 for all) after adjustments for the other physical fitness components, age, body mass index, and socioeconomic status, in both genders. Our findings highlight the importance of promoting and developing flexibility, as well as the other health-related physical fitness components in schoolchildren to reach adequate levels of MC. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Genotoxicity of 2-[2-(acetylamino)-4-[bis(2-hydroxyethyl)amino]-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-6) and 4-amino-3,3'-dichloro-5,4'-dinitro-biphenyl (ADDB) in goldfish (Carassius auratus) using the micronucleus test and the comet assay.

PubMed

Masuda, Shuichi; Deguchi, Yuya; Masuda, Yumi; Watanabe, Tetsushi; Nukaya, Haruo; Terao, Yoshiyasu; Takamura, Takeji; Wakabayashi, Keiji; Kinae, Naohide

2004-05-09

2-[2-(Acetylamino)-4-[bis(2-hydroxyethyl)amino]-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-6) and 4-amino-3,3'-dichloro-5,4'-dinitrobiphenyl (ADDB) are two compounds, which show strong mutagenicity toward bacteria, that have been identified as major mutagens in river water in Japan. In the present study, we examined the genotoxicity of PBTA-6 and ADDB in goldfish (Carassius auratus) by the micronucleus test and single-cell gel electrophoresis (comet assay). The frequencies of micronuclei in gill cells gradually increased until 96h after i.p. injection of PBTA-6 and ADDB at doses of 50mg/kg body weight, and then decreased 144h after injection. PBTA-6 induced micronuclei in gill cells dose-dependently at a dose range of 1-100mg/kg body weight, giving significantly high frequencies at doses of 50 and 100mg/kg body weight. On the other hand, no significant increase was observed in the peripheral erythrocytes of goldfish exposed to PBTA-6 or ADDB. In the comet assay, values of DNA tail moment and tail length in peripheral erythrocytes increased significantly until 6h after the i.p. injection of PBTA-6 (50mg/kg body weight), only to decrease by 9h after injection. Both the DNA tail moment and tail length were dose-dependently increased by injections of PBTA-6 at doses ranging from 1 to 50mg/kg. Significantly high values for tail moment and tail length were found in peripheral erythrocytes 3h after an i.p. injection of ADDB and persisted for up to 6h. These results show that both PBTA-6 and ADDB have genotoxic effects in goldfish.

Space Shuttle Orbiter SILTS Pod Flow Angularity and Aerodynamic Heating Tests (OH-102A and OH-400).

DTIC Science & Technology

1979-11-01

fabricated from 17 - 4PH stainless steel and instrumented with tnermocouples. A photograph or the 9L-p model with the U.UJZJ scale vertical tail installed is...DISTRIBUTION STATE=MENT (of this ’Report) Approved for public release; distribution unlimited. 17 . DISTRIBUTION STATEMENT (of the abstract entered In...Model Installation ....... .................. . 17 3. Vertical Tail for Flow Angularity ..... .............. ... 18 4. Photograph of 56-) Model

49 CFR 178.817 - Righting test.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 3 2013-10-01 2013-10-01 false Righting test. 178.817 Section 178.817... Righting test. (a) General. The righting test must be conducted for the qualification of all flexible IBCs designed to be lifted from the top or side. (b) Special preparation for the righting test. The flexible IBC...

49 CFR 178.1070 - Tear test.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 3 2013-10-01 2013-10-01 false Tear test. 178.1070 Section 178.1070... Containers § 178.1070 Tear test. (a) General. The tear test must be conducted for the qualification of all of Flexible Bulk Containers design types. (b) Special preparation for the tear test. Flexible Bulk Container...

Tachykinin NK-1 and NK-3 selective agonists induce analgesia in the formalin test for tonic pain following intra-VTA or intra-accumbens microinfusions.

PubMed

Altier, N; Stewart, J

1997-12-01

Experiments were designed to examine the analgesic effects induced by selective tachykinin receptor agonists microinfused into either the ventral tegmental area (VTA) or nucleus accumbens septi (NAS). Rats were tested in the formalin test for tonic pain following an injection of 0.05 ml of 2.5% formalin into one hind paw immediately after bilateral intra-VTA infusions of either the NK-1 agonist, GR-73632 (0.005, 0.05 or 0.5 nmol/side), the NK-3 agonist, senktide (0.005, 0.5 or 1.5 nmol/side), or saline. Two weeks later, the saline-treated rats were assessed in the tail-flick test for phasic pain after infusions of the tachykinin agonists. Tail-flick latencies were recorded following immersion of the tail in 55 degrees C hot water at 10 min intervals for 1 h immediately after intra-VTA infusions of either GR-73632 (0.5 nmol/side), senktide (1.5 nmol/side) or saline. In a second group of rats, the same effects were studied after infusions into the nucleus accumbens (NAS) of GR-73632 (0.005, 0.5 or 1.5 nmol/side), senktide (0.005, 0.5 or 1.5 nmol/side), or saline. In both the VTA and NAS, the NK-1 and the NK-3 agonists caused significant analgesia in the formalin test, although the NK-1 agonist appeared to be more effective. Naltrexone (2.0 mg/kg) pretreatment failed to reverse the analgesic effects in the formalin test induced by intra-VTA infusions of the substance P (SP) analog, DiMe-C7 (3.0 microg/side), GR-73632 (0.5 nmol/side), or senktide (1.5 nmol/side). Neither compound given at either site was effective in the tail-flick test. These findings suggest that SP-dopamine (DA) interactions within the mesolimbic DA system play an important role in the inhibition of tonic pain. Furthermore, they support our earlier ideas that activation of midbrain DA systems by SP might play a role in stress- and/or pain-induced analgesia.

Microbial communities in low permeability, high pH uranium mine tailings: characterization and potential effects.

PubMed

Bondici, V F; Lawrence, J R; Khan, N H; Hill, J E; Yergeau, E; Wolfaardt, G M; Warner, J; Korber, D R

2013-06-01

To describe the diversity and metabolic potential of microbial communities in uranium mine tailings characterized by high pH, high metal concentration and low permeability. To assess microbial diversity and their potential to influence the geochemistry of uranium mine tailings using aerobic and anaerobic culture-based methods, in conjunction with next generation sequencing and clone library sequencing targeting two universal bacterial markers (the 16S rRNA and cpn60 genes). Growth assays revealed that 69% of the 59 distinct culturable isolates evaluated were multiple-metal resistant, with 15% exhibiting dual-metal hypertolerance. There was a moderately positive correlation coefficient (R = 0·43, P < 0·05) between multiple-metal resistance of the isolates and their enzyme expression profile. Of the isolates tested, 17 reduced amorphous iron, 22 reduced molybdate and seven oxidized arsenite. Based on next generation sequencing, tailings depth was shown to influence bacterial community composition, with the difference in the microbial diversity of the upper (0-20 m) and middle (20-40 m) tailings zones being highly significant (P < 0·01) from the lower zone (40-60 m) and the difference in diversity of the upper and middle tailings zone being significant (P < 0·05). Phylotypes closely related to well-known sulfate-reducing and iron-reducing bacteria were identified with low abundance, yet relatively high diversity. The presence of a population of metabolically-diverse, metal-resistant micro-organisms within the tailings environment, along with their demonstrated capacity for transforming metal elements, suggests that these organisms have the potential to influence the long-term geochemistry of the tailings. This study is the first investigation of the diversity and functional potential of micro-organisms present in low permeability, high pH uranium mine tailings. © 2013 The Society for Applied Microbiology.

Environmental hazard assessment of a marine mine tailings deposit site and potential implications for deep-sea mining.

PubMed

Mestre, Nélia C; Rocha, Thiago L; Canals, Miquel; Cardoso, Cátia; Danovaro, Roberto; Dell'Anno, Antonio; Gambi, Cristina; Regoli, Francesco; Sanchez-Vidal, Anna; Bebianno, Maria João

2017-09-01

Portmán Bay is a heavily contaminated area resulting from decades of metal mine tailings disposal, and is considered a suitable shallow-water analogue to investigate the potential ecotoxicological impact of deep-sea mining. Resuspension plumes were artificially created by removing the top layer of the mine tailings deposit by bottom trawling. Mussels were deployed at three sites: i) off the mine tailings deposit area; ii) on the mine tailings deposit beyond the influence from the resuspension plumes; iii) under the influence of the artificially generated resuspension plumes. Surface sediment samples were collected at the same sites for metal analysis and ecotoxicity assessment. Metal concentrations and a battery of biomarkers (oxidative stress, metal exposure, biotransformation and oxidative damage) were measured in different mussel tissues. The environmental hazard posed by the resuspension plumes was investigated by a quantitative weight of evidence (WOE) model that integrated all the data. The resuspension of sediments loaded with metal mine tails demonstrated that chemical contaminants were released by trawling subsequently inducing ecotoxicological impact in mussels' health. Considering as sediment quality guidelines (SQGs) those indicated in Spanish action level B for the disposal of dredged material at sea, the WOE model indicates that the hazard is slight off the mine tailings deposit, moderate on the mine tailings deposit without the influence from the resuspension plumes, and major under the influence of the resuspension plumes. Portmán Bay mine tailings deposit is a by-product of sulphide mining, and despite differences in environmental setting, it can reflect the potential ecotoxic effects to marine fauna from the impact of resuspension of plumes created by deep-sea mining of polymetallic sulphides. A similar approach as in this study could be applied in other areas affected by sediment resuspension and for testing future deep-sea mining sites in order to assess the associated environmental hazards. Copyright © 2017 Elsevier Ltd. All rights reserved.

Examination of Body Composition, Flexibility, Balance, and Concentration Related to Dance Exercise

ERIC Educational Resources Information Center

Bastug, Gulsum

2018-01-01

In this study was to examine the body composition, flexibility, balance and concentration characteristics of dance exercise. Total of 268 university students whose average age was 20.59 ± 1.59 years were included. Height measurements, body weight measurements, flexibility measurements, balance test, concentration test of the students who had dance…

A Study of Airline Passenger Susceptibility to Atmospheric Turbulence Hazard

NASA Technical Reports Server (NTRS)

Stewart, Eric C.

2000-01-01

A simple, generic, simulation math model of a commercial airliner has been developed to study the susceptibility of unrestrained passengers to large, discrete gust encounters. The math model simulates the longitudinal motion to vertical gusts and includes (1) motion of an unrestrained passenger in the rear cabin, (2) fuselage flexibility, (3) the lag in the downwash from the wing to the tail, and (4) unsteady lift effects. Airplane and passenger response contours are calculated for a matrix of gust amplitudes and gust lengths of a simulated mountain rotor. A comparison of the model-predicted responses to data from three accidents indicates that the accelerations in actual accidents are sometimes much larger than the simulated gust encounters.

Migration and speciation of heavy metal in salinized mine tailings affected by iron mining.

PubMed

Zhang, Xu; Yang, Huanhuan; Cui, Zhaojie

2017-10-01

The negative effects of heavy metals have aroused much attention due to their high toxicity to human beings. Migration and transformation trend of heavy metals have a close relationship with soil safety. Researching on migration and transformation of heavy metals in tailings can provide a reliable basis for pollution management and ecosystem restoration. Heavy metal speciation plays an important role in risk assessment. We chose Anshan tailings for our study, including field investigations and laboratory research. Four typical heavy metal elements of mine tailings {Fe (373.89 g/kg), Mn (2,303.80 mg/kg), Pb (40.99 mg/kg) and Cr (199.92 mg/kg)} were studied via Tessier test in vertical and horizontal direction. The main speciation of heavy metals in Anshan tailings was the residual. However, heavy metals have a strong ability for migration and transformation in vertical and horizontal directions. Its tendency to change from stable to unstable speciation results in increasing bioavailability and potential bioavailability. Fe, Mn, Pb and Cr showed different ability in the migration and transformation process (Mn > Pb > Fe > Cr) depending on the characteristics of heavy metals and physicochemical properties of the environment.

An analysis on 45° sweep tail angle for blended wing body aircraft to the aerodynamics coefficients by wind tunnel experiment

NASA Astrophysics Data System (ADS)

Latif, M. Z. A. Abd; Ahmad, M. A.; Nasir, R. E. Mohd; Wisnoe, W.; Saad, M. R.

2017-12-01

This paper presents the analysis of a model from UiTM Blended Wing Body (BWB) UAV, Baseline V that has been tested at UPNM high speed wind tunnel. Baseline V has a unique design due to different NACA sections used for its fuselage, body, wing root, midwing, wingtip, tail root, tail tip and the tail is swept 45° backward. The purpose of this experiment is to study the aerodynamic characteristics when the tail sweeps 45° backward. The experiments are conducted several times using 71.5% scaled down model at about 49.58 m/s airspeed or 25 Hz. The tail angle deflection is fixed and set at zero angle. All the data obtained is analyzed and presented in terms of coefficient of lift, coefficient of drag and also lift-to-drag ratio, and is plotted against various angles of attack. The angles of attack used for this experiments are between -10° to +30°. The blockage correction such as solid blockage, wake blockage and streamline curvature blockage are calculated in order to obtain true performance of the aircraft. From the observation, Baseline V shows that the aircraft tends to stall at around +15°. The maximum L/D ratio achieved for Baseline V is 20.8, however it decreases slightly to 20.7 after blockage corrections.

Pb uptake and toxicity to Iris halophila tested on Pb mine tailing materials.

PubMed

Han, Yulin; Zhang, Lili; Yang, Yongheng; Yuan, Haiyan; Zhao, Jiuzhou; Gu, Jiguang; Huang, Suzhen

2016-07-01

Pb tolerant mechanisms, plant physiological response and Pb sub-cellular localization in the root cells of Iris halophila were studied in sand culture and the Pb mine tailings. Results showed that the activities of superoxide dismutase (SOD) and peroxidase (POD) in the underground parts and the activity of catalase (CAT) in the aboveground and underground parts increased as Pb level was enhanced. Glutathione (GSH) and ascorbic acid (AsA) contents increased by Pb treatments. Pb deposits were found in the middle cell walls or along the inner side of epibiotic protoplasm of some cells which accumulated a large quantity of Pb and died. The dry weights (DWs) of aboveground parts under all Pb tailings treatments decreased insignificantly, while the DW of the underground parts growing in the pure Pb tailings decreased significantly. Pb, Cu, Cd, and Zn contents increased significantly as the levels of Pb tailings were enhanced and Pb contents in the aboveground and underground parts reached 64.75 and 751.75 μg/g DW, respectively, at pure Pb tailings treatment. The results indicated that I. halophila is a promising plant in the phytoremediation of Pb contaminated environment. Some antioxidant enzymes, antioxidants and compartmentalization of Pb were played major roles in Pb tolerance of I. halophila. Copyright © 2016 Elsevier Ltd. All rights reserved.

Wind Tunnel Investigation of the Effects of Surface Porosity and Vertical Tail Placement on Slender Wing Vortex Flow Aerodynamics at Supersonic Speeds

NASA Technical Reports Server (NTRS)

Erickson, Gary E.

2007-01-01

A wind tunnel experiment was conducted in the NASA Langley Research Center (LaRC) Unitary Plan Wind Tunnel (UPWT) to determine the effects of passive surface porosity and vertical tail placement on vortex flow development and interactions about a general research fighter configuration at supersonic speeds. Optical flow measurement and flow visualization techniques were used that featured pressure sensitive paint (PSP), laser vapor screen (LVS), and schlieren, These techniques were combined with conventional electronically-scanned pressure (ESP) and six-component force and moment measurements to quantify and to visualize the effects of flow-through porosity applied to a wing leading edge extension (LEX) and the placement of centerline and twin vertical tails on the vortex-dominated flow field of a 65 cropped delta wing model. Test results were obtained at free-stream Mach numbers of 1.6, 1.8, and 2.1 and a Reynolds number per foot of 2.0 million. LEX porosity promoted a wing vortex-dominated flow field as a result of a diffusion and weakening of the LEX vortex. The redistribution of the vortex-induced suction pressures contributed to large nose-down pitching moment increments but did not significantly affect the vortex-induced lift. The trends associated with LEX porosity were unaffected by vertical tail placement. The centerline tail configuration generally provided more stable rolling moments and yawing moments compared to the twin wing-mounted vertical tails. The strength of a complex system of shock waves between the twin tails was reduced by LEX porosity.

Evaluation of in situ layers for treatment of acid mine drainage: a field comparison.

PubMed

Hulshof, Andrea H M; Blowes, David W; Gould, W Douglas

2006-05-01

Reactive treatment layers, containing labile organic carbon, were evaluated to determine their ability to promote sulfate reduction and metal sulfide precipitation within a tailings impoundment, thereby treating tailings effluent prior to discharge. Organic carbon materials, including woodchips and pulp waste, were mixed with the upper meter of tailings in two separate test cells, a third control cell contained only tailings. In the woodchip cell sulfate reduction rates were 500 mg L-1a-1, (5.2 mmol L-1a-1) this was coupled with the gradual removal of 350 mg L-1 Zn (5.4 mmol L-1). Decreased delta13CDIC values from -3 per thousand to as low as -12 per thousand indicated that sulfate reduction was coupled with organic carbon oxidation. In the pulp waste cell the most dramatic change was observed near the interface between the pulp waste amended tailings and the underlying undisturbed tailings. Sulfate reduction rates were 5000 mg L-1a-1 (52 mmol L-1a-1), Fe concentrations decreased by 80-99.5% (148 mmol L-1) and Zn was consistently <5 mg L-1. Rates of sulfate reduction and metal removal decreased as the pore water migrated upward into the shallower tailings. Increased rates of sulfate reduction in the pulp waste cell were consistent with decreased delta13CDIC values, to as low as -22 per thousand, and increased populations of sulfate reducing bacteria. Lower concentrations of the nutrients, phosphorus, organic carbon and nitrogen in the woodchip material contribute to the lower sulfate reduction rates observed in the woodchip cell.

KSC-07pd3629

NASA Image and Video Library

2007-12-14

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A, a technician prepares a cable from an electrical harness in space shuttle Atlantis' aft main engine compartment connected with the engine cut-off, or ECO, system leading into the tail mast. The test wiring leads from the tail mast to the interior of the mobile launcher platform where the Time Domain Reflectometry, or TDR, test equipment will be located to test the sensor system. The shuttle's planned launches on Dec. 6 and Dec. 9 were postponed because of false readings from the part of the ECO system that monitors the liquid hydrogen section of the tank. The liftoff date from NASA's Kennedy Space Center, Florida, is now targeted for Jan. 10, depending on the resolution of the problem in the fuel sensor system. Photo credit: NASA/Kim Shiflett

KSC-07pd3628

NASA Image and Video Library

2007-12-14

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A, cables lead from an electrical harness in space shuttle Atlantis' aft main engine compartment connected with the engine cut-off, or ECO, system into the tail mast. The test wiring leads from the tail mast to the interior of the mobile launcher platform where the Time Domain Reflectometry, or TDR, test equipment will be located to test the sensor system. The shuttle's planned launches on Dec. 6 and Dec. 9 were postponed because of false readings from the part of the ECO system that monitors the liquid hydrogen section of the tank. The liftoff date from NASA's Kennedy Space Center, Florida, is now targeted for Jan. 10, depending on the resolution of the problem in the fuel sensor system. Photo credit: NASA/Kim Shiflett

KSC-07pd3627

NASA Image and Video Library

2007-12-14

KENNEDY SPACE CENTER, FLA. -- On Launch Pad 39A, cables lead from an electrical harness in space shuttle Atlantis' aft main engine compartment connected with the engine cut-off, or ECO, system into the tail mast. The test wiring leads from the tail mast to the interior of the mobile launcher platform where the Time Domain Reflectometry, or TDR, test equipment will be located to test the sensor system. The shuttle's planned launches on Dec. 6 and Dec. 9 were postponed because of false readings from the part of the ECO system that monitors the liquid hydrogen section of the tank. The liftoff date from NASA's Kennedy Space Center, Florida, is now targeted for Jan. 10, depending on the resolution of the problem in the fuel sensor system. Photo credit: NASA/Kim Shiflett

Undulatory Swimming Performance and Body Stiffness Modulation in a Soft Robotic Fish-Inspired Physical Model.

PubMed

Jusufi, Ardian; Vogt, Daniel M; Wood, Robert J; Lauder, George V

2017-09-01

Undulatory motion of the body is the dominant mode of locomotion in fishes, and numerous studies of body kinematics and muscle activity patterns have provided insights into the mechanics of swimming. However, it has not been possible to investigate how key parameters such as the extent of bilateral muscle activation affect propulsive performance due to the inability to manipulate muscle activation in live, freely swimming fishes. In this article we extend previous work on passive flexible mechanical models of undulatory propulsion by using actively controlled pneumatic actuators attached to a flexible foil to gain insight into undulatory locomotion and mechanisms for body stiffness control. Two soft actuators were attached on each side of a flexible panel with stiffness comparable to that of a fish body. To study how bilateral contraction can be used to modify axial body stiffness during swimming, we ran a parameter sweep of actuator contraction phasing and frequency. Thrust production by the soft pneumatic actuators was tested at cyclic undulation frequencies ranging from 0.3 to 1.2 Hz in a recirculating flow tank at flow speeds up to 28 cm/s. Overall, this system generated more thrust at higher tail beat frequencies, with a plateau in thrust above 0.8 Hz. Self-propelled speed was found to be 0.8 foil lengths per second or ∼13 cm/s when actuated at 0.55 Hz. This active pneumatic model is capable of producing substantial trailing edge amplitudes with a maximum excursion equivalent to 1.4 foil lengths, and of generating considerable thrust. Altering the extent of bilateral co-contraction in a range from -22% to 17% of the cycle period showed that thrust was maximized with some amount of simultaneous left-right actuation of ∼3% to 6% of the cycle period. When the system is exposed to water flow, thrust was substantially reduced for conditions of greatest antagonistic overlap in left-right actuation, and also for the largest latencies introduced. This experimental platform provides a soft robotic testbed for studying aquatic propulsion with active control of undulatory kinematics.

Leachability of Arsenic and Heavy Metals from Mine Tailings of Abandoned Metal Mines

PubMed Central

Lim, Mihee; Han, Gi-Chun; Ahn, Ji-Whan; You, Kwang-Suk; Kim, Hyung-Seok

2009-01-01

Mine tailings from an abandoned metal mine in Korea contained high concentrations of arsenic (As) and heavy metals [e.g., As: 67,336, Fe: 137,180, Cu: 764, Pb: 3,572, and Zn: 12,420 (mg/kg)]. US EPA method 6010 was an effective method for analyzing total arsenic and heavy metals concentrations. Arsenic in the mine tailings showed a high residual fraction of 89% by a sequential extraction. In Toxicity Characteristic Leaching Procedure (TCLP) and Korean Standard Leaching Test (KSLT), leaching concentrations of arsenic and heavy metals were very low [e.g., As (mg/L): 0.4 for TCLP and 0.2 for KSLT; cf. As criteria (mg/L): 5.0 for TCLP and 1.5 for KSLT]. PMID:20049231

Full-scale Wind-tunnel Research on Tail Buffeting and Wing-fuselage Interference of a Low-wing Monoplane

NASA Technical Reports Server (NTRS)

Hood, Manley J; White, James A

1933-01-01

Some preliminary results of full scale wind tunnel testing to determine the best means of reducing the tail buffeting and wing-fuselage interference of a low-wing monoplane are given. Data indicating the effects of an engine cowling, fillets, auxiliary airfoils of short span, reflexes trailing edge, propeller slipstream, and various combinations of these features are included. The best all-round results were obtained by the use of fillets together with the National Advisory Committee for Aeronautics (NACA) cowling. This combination reduced the tail buffeting oscillations to one-fourth of their original amplitudes, increased the maximum lift 11 percent, decreased the minimum drag 9 percent, and increased the maximum ratio of lift to drag 19 percent.

Interference of Tail Surfaces and Wing and Fuselage from Tests of 17 Combinations in the N.A.C.A. Variable-Density Tunnel

NASA Technical Reports Server (NTRS)

Sherman, Albert

1939-01-01

An investigation of the interference associated with tail surfaces added to wing-fuselage combinations was included in the interference program in progress in the NACA variable-density tunnel. The results indicate that, in aerodynamically clean combinations, the increment of the high-speed drag can be estimated from section characteristics within useful limits of accuracy. The interference appears mainly as effects on the downwash angle and as losses in the tail effectiveness and varies with the geometry of the combination. An interference burble, which markedly increases the glide-path angle and the stability in pitch before the actual stall, may be considered a means of obtaining satisfactory stalling characteristics for complete combination.

Pavement testing facility : effects of tire pressure on flexible pavement response performance

DOT National Transportation Integrated Search

1989-08-01

The effects of tire pressure on flexible pavement response and performance were evaluated using data from the first phase of research at the Federal Highway Administration's Pavement Testing Facility. The Accelerated Loading Facility testing machine ...

Static Wind-Tunnel and Radio-Controlled Flight Test Investigation of a Remotely Piloted Vehicle Having a Delta Wing Planform

NASA Technical Reports Server (NTRS)

Yip, Long P.; Fratello, David J.; Robelen, David B.; Makowiec, George M.

1990-01-01

At the request of the United States Marine Corps, an exploratory wind-tunnel and flight test investigation was conducted by the Flight Dynamics Branch at the NASA Langley Research Center to improve the stability, controllability, and general flight characteristics of the Marine Corps Exdrone RPV (Remotely Piloted Vehicle) configuration. Static wind tunnel tests were conducted in the Langley 12 foot Low Speed Wind Tunnel to identify and improve the stability and control characteristics of the vehicle. The wind tunnel test resulted in several configuration modifications which included increased elevator size, increased vertical tail size and tail moment arm, increased rudder size and aileron size, the addition of vertical wing tip fins, and the addition of leading-edge droops on the outboard wing panel to improve stall departure resistance. Flight tests of the modified configuration were conducted at the NASA Plum Tree Test Site to provide a qualitative evaluation of the flight characteristics of the modified configuration.

A low speed wind tunnel test of a 0.050 scale model of shuttle orbiter (model 089B) to investigate the longitudinal and lateral directional effects of canard and tail configurational modifications in the LTV LSWT (MA14)

NASA Technical Reports Server (NTRS)

Chambliss, E. B.

1976-01-01

A low speed wind tunnel test was conducted to determine the effects of 6 canard configurations on the 0.050 scale model of shuttle orbiter 089B. In addition, two horizontal tail configurations were tested at two positions on the model as were two wing configurations. Since this test was restricted to 103 runs, only a limited number of permutations of the configurational changes could be tested. The testing was done in the 15 by 20 foot section of the LSWT and consisted of pitch polars, one yawed polar and several yaw runs. The pitch polars encompassed an alpha range from 0 to 28 deg; the yawed polar was run at beta = +2 degrees and the yaw runs covered a beta range from -6 to +6 deg at angles-of-attack of 0, 4, 10, 16, and 20 deg.

49 CFR 178.818 - Tear test.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 3 2013-10-01 2013-10-01 false Tear test. 178.818 Section 178.818 Transportation... Tear test. (a) General. The tear test must be conducted for the qualification of all flexible IBC design types. (b) Special preparation for the tear test. The flexible IBC must be filled to not less than...

49 CFR 178.818 - Tear test.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Tear test. 178.818 Section 178.818 Transportation... § 178.818 Tear test. (a) General. The tear test must be conducted for the qualification of all flexible IBC design types. (b) Special preparation for the tear test. The flexible IBC must be filled to not...

Formation and evolution of substructures in tidal tails: spherical dark matter haloes

NASA Astrophysics Data System (ADS)

Reinoso, B.; Fellhauer, M.; Véjar, R.

2018-05-01

Recently a theory about the formation of overdensities of stars along tidal tails of globular clusters has been presented. This theory predicts the position and the time of the formation of such overdensities and was successfully tested with N-body simulations of globular clusters in a point-mass galactic potential. In this work, we present a comparison between this theory and our simulations using a dwarf galaxy orbiting two differently shaped dark matter haloes to study the effects of a cored and a cuspy halo on the formation and the evolution of tidal tails. We find no difference using a cuspy or a cored halo, however, we find an intriguing asymmetry between the leading arm and the trailing arm of the tidal tails. The trailing arm grows faster than the leading arm. This asymmetry is seen in the distance to the first overdensity and its size as well. We establish a relation between the distance to the first overdensity and the size of this overdensity.

[Effects of bio-crust on soil microbial biomass and enzyme activities in copper mine tailings].

PubMed

Chen, Zheng; Yang, Gui-de; Sun, Qing-ye

2009-09-01

Bio-crust is the initial stage of natural primary succession in copper mine tailings. With the Yangshanchong and Tongguanshan copper mine tailings in Tongling City of Anhui Province as test objects, this paper studied the soil microbial biomass C and N and the activities of dehydrogenase, catalase, alkaline phosphatase, and urease under different types of bio-crust. The bio-crusts improved the soil microbial biomass and enzyme activities in the upper layer of the tailings markedly. Algal crust had the best effect in improving soil microbial biomass C and N, followed by moss-algal crust, and moss crust. Soil microflora also varied with the type of bio-crust. No'significant difference was observed in the soil enzyme activities under the three types of bio-crust. Soil alkaline phosphatase activity was significantly positively correlated with soil microbial biomass and dehydrogenase and urease activities, but negatively correlated with soil pH. In addition, moss rhizoid could markedly enhance the soil microbial biomass and enzyme activities in moss crust rhizoid.

Experimental measurement of dolphin thrust generated during a tail stand using DPIV

NASA Astrophysics Data System (ADS)

Wei, Timothy; Fish, Frank; Williams, Terrie; Wu, Vicki; Sherman, Erica; Misfeldt, Mitchel; Ringenberg, Hunter; Rogers, Dylan

2016-11-01

The thrust generated by dolphins doing tail stands was measured using DPIV. The technique entailed measuring vortex strength associated with the tail motion and correlating it to above water video sequences showing the amount of the dolphin's body that was being lifted out of the water. The underlying drivers for this research included: i) understanding the physiology, hydrodynamics and efficiency of dolphin locomotion, ii) developing non-invasive measurement techniques for studying marine swimming and iii) quantifying the actual propulsive capabilities of these animals. Two different bottlenose dolphins at the Long Marine Lab at UC-Santa Cruz were used as test subjects. Application of the Kutta-Joukowski Theorem on measured vortex circulations yielded thrust values that were well correlated with estimates of dolphin body weight being supported above water. This demonstrates that the tail motion can be interpreted as a flapping hydrofoil that can generate a sustained thrust roughly equal to the dolphin's weight. Videos of DPIV measurements overlaid with the dolphins will be presented along with thrust/weight data.

Quality Test of Flexible Flat Cable (FFC) With Short Open Test Using Law Ohm Approach through Embedded Fuzzy Logic Based On Open Source Arduino Data Logger

NASA Astrophysics Data System (ADS)

Rohmanu, Ajar; Everhard, Yan

2017-04-01

A technological development, especially in the field of electronics is very fast. One of the developments in the electronics hardware device is Flexible Flat Cable (FFC), which serves as a media liaison between the main boards with other hardware parts. The production of Flexible Flat Cable (FFC) will go through the process of testing and measuring of the quality Flexible Flat Cable (FFC). Currently, the testing and measurement is still done manually by observing the Light Emitting Diode (LED) by the operator, so there were many problems. This study will be made of test quality Flexible Flat Cable (FFC) computationally utilize Open Source Embedded System. The method used is the measurement with Short Open Test method using Ohm’s Law approach to 4-wire (Kelvin) and fuzzy logic as a decision maker measurement results based on Open Source Arduino Data Logger. This system uses a sensor current INA219 as a sensor to read the voltage value thus obtained resistance value Flexible Flat Cable (FFC). To get a good system we will do the Black-box testing as well as testing the accuracy and precision with the standard deviation method. In testing the system using three models samples were obtained the test results in the form of standard deviation for the first model of 1.921 second model of 4.567 and 6.300 for the third model. While the value of the Standard Error of Mean (SEM) for the first model of the model 0.304 second at 0.736 and 0.996 of the third model. In testing this system, we will also obtain the average value of the measurement tolerance resistance values for the first model of - 3.50% 4.45% second model and the third model of 5.18% with the standard measurement of prisoners and improve productivity becomes 118.33%. From the results of the testing system is expected to improve the quality and productivity in the process of testing Flexible Flat Cable (FFC).

Flexible ferroelectric element based on van der Waals heteroepitaxy.

PubMed

Jiang, Jie; Bitla, Yugandhar; Huang, Chun-Wei; Do, Thi Hien; Liu, Heng-Jui; Hsieh, Ying-Hui; Ma, Chun-Hao; Jang, Chi-Yuan; Lai, Yu-Hong; Chiu, Po-Wen; Wu, Wen-Wei; Chen, Yi-Chun; Zhou, Yi-Chun; Chu, Ying-Hao

2017-06-01

We present a promising technology for nonvolatile flexible electronic devices: A direct fabrication of epitaxial lead zirconium titanate (PZT) on flexible mica substrate via van der Waals epitaxy. These single-crystalline flexible ferroelectric PZT films not only retain their performance, reliability, and thermal stability comparable to those on rigid counterparts in tests of nonvolatile memory elements but also exhibit remarkable mechanical properties with robust operation in bent states (bending radii down to 2.5 mm) and cycling tests (1000 times). This study marks the technological advancement toward realizing much-awaited flexible yet single-crystalline nonvolatile electronic devices for the design and development of flexible, lightweight, and next-generation smart devices with potential applications in electronics, robotics, automotive, health care, industrial, and military systems.

Flexible ferroelectric element based on van der Waals heteroepitaxy

PubMed Central

Jiang, Jie; Bitla, Yugandhar; Huang, Chun-Wei; Do, Thi Hien; Liu, Heng-Jui; Hsieh, Ying-Hui; Ma, Chun-Hao; Jang, Chi-Yuan; Lai, Yu-Hong; Chiu, Po-Wen; Wu, Wen-Wei; Chen, Yi-Chun; Zhou, Yi-Chun; Chu, Ying-Hao

2017-01-01

We present a promising technology for nonvolatile flexible electronic devices: A direct fabrication of epitaxial lead zirconium titanate (PZT) on flexible mica substrate via van der Waals epitaxy. These single-crystalline flexible ferroelectric PZT films not only retain their performance, reliability, and thermal stability comparable to those on rigid counterparts in tests of nonvolatile memory elements but also exhibit remarkable mechanical properties with robust operation in bent states (bending radii down to 2.5 mm) and cycling tests (1000 times). This study marks the technological advancement toward realizing much-awaited flexible yet single-crystalline nonvolatile electronic devices for the design and development of flexible, lightweight, and next-generation smart devices with potential applications in electronics, robotics, automotive, health care, industrial, and military systems. PMID:28630922

Effects of William training on lumbosacral muscles function, lumbar curve and pain.

PubMed

Fatemi, Rouholah; Javid, Marziyeh; Najafabadi, Ebrahim Moslehi

2015-01-01

There are many types of treatments and recommendations for restoring back deformities depending on doctors' knowledge and opinions. The purpose of the exercises is to reduce pain and to ensure stability of the lower trunk by toning the abdominal muscles, buttocks and hamstrings. Given the duration of flares and relapses rate, it is important to apply an efficient and lasting treatment. To evaluate the effects of 8 weeks of William's training on flexibility of lumbosacral muscles and lumbar angle in females with Hyperlordosis. Forty female students with lumbar lordosis more than normal degrees (Hyperlordotic) that were randomly divided into exercise and control groups were selected as the study sample. The lumbar lordosis was measured using a flexible ruler, flexibility of hamstring muscles was measured with the active knee extension test, the hip flexor muscles strength was measured using Thomas test, the lumbar muscles flexibility measures by Schober test, abdominal muscles strength measured by Sit-Up test and back pain was measured using McGill's Visual Analogue Scales (VAS) questionnaire. Data were compared before and post-test using independent and paired t-testes. Results showed that 8 weeks of William's exercise led to significant decreases in lumbar angle and back pain, increases in flexibility of hamstring muscles, hip flexor muscles flexibility, lumbar extensor muscles flexibility and abdominal muscles strength. The findings show that William's corrective training can be considered as a useful and valid method for restoring and refining back deformities like as accentuated back-arc and became wreaked muscles' performance in lumbar areas.

Verification of component mode techniques for flexible multibody systems

NASA Technical Reports Server (NTRS)

Wiens, Gloria J.

1990-01-01

Investigations were conducted in the modeling aspects of flexible multibodies undergoing large angular displacements. Models were to be generated and analyzed through application of computer simulation packages employing the 'component mode synthesis' techniques. Multibody Modeling, Verification and Control Laboratory (MMVC) plan was implemented, which includes running experimental tests on flexible multibody test articles. From these tests, data was to be collected for later correlation and verification of the theoretical results predicted by the modeling and simulation process.

Low-speed static and dynamic force tests of a generic supersonic cruise fighter configuration

NASA Technical Reports Server (NTRS)

Hahne, David E.

1989-01-01

Static and dynamic force tests of a generic fighter configuration designed for sustained supersonic flight were conducted in the Langley 30- by 60-foot tunnel. The baseline configuration had a 65 deg arrow wing, twin wing mounted vertical tails and a canard. Results showed that control was available up to C sub L,max (maximum lift coefficient) from aerodynamic controls about all axes but control in the pitch and yaw axes decreased rapidly in the post-stall angle-of-attack region. The baseline configuration showed stable lateral-directional characteristics at low angles of attack but directional stability occurred near alpha = 25 deg as the wing shielded the vertical tails. The configuration showed positive effective dihedral throughout the test angle-of-attack range. Forced oscillation tests indicated that the baseline configuration had stable damping characteristics about the lateral-directional axes.

Survival and reproductive rate of mites in relation to resistance of their barn swallow hosts.

PubMed

Møller, A P

2000-08-01

Parasite resistance may act via a number of different mechanisms that regulate or control the survival and the reproductive rate of parasites. Observations and experiments were used to test for effects of host resistance on parasite survival and rate of reproduction. Natural levels of infestation of barn swallow Hirundo rustica nests by the tropical fowl mite Ornithonyssus bursa were positively related to brood size, inversely related to the length of the outermost tail feathers of male nest owners (a secondary sexual character) and affected by time of reproduction by the host. A mite inoculation experiment, in which 50 adult mites were introduced into nests during the laying period of the host, was used to test for differential survival and reproduction of mites as a function of host resistance. The relationship between survival and reproduction of parasites, male tail length and host resistance was investigated. There was a negative relationship between mite numbers per nest after fledging of nestlings and male tail length. This relationship was mainly caused by a reduction in the number of mites in the first and second nymph stage with increasing tail length of male hosts, implying a reduction in rate of reproduction of mites. The proportion of mites that had recently fed was inversely related to tail length of male hosts. The proportion of nymph stages was positively related to the proportion of mites that had recently had a blood meal. Parasite resistance of barn swallows to the tropical fowl mite thus appeared to act through increased mortality rate of adult and nymph stages of mites, and through reduced reproductive rates of mites on resistant hosts. This is the first study demonstating a direct relationship between fitness components of a parasite and the expression of a secondary sexual character of a host.

An Exploration Based Cognitive Bias Test for Mice: Effects of Handling Method and Stereotypic Behaviour.

PubMed

Novak, Janja; Bailoo, Jeremy D; Melotti, Luca; Rommen, Jonas; Würbel, Hanno

2015-01-01

Behavioural tests to assess affective states are widely used in human research and have recently been extended to animals. These tests assume that affective state influences cognitive processing, and that animals in a negative affective state interpret ambiguous information as expecting a negative outcome (displaying a negative cognitive bias). Most of these tests however, require long discrimination training. The aim of the study was to validate an exploration based cognitive bias test, using two different handling methods, as previous studies have shown that standard tail handling of mice increases physiological and behavioural measures of anxiety compared to cupped handling. Therefore, we hypothesised that tail handled mice would display a negative cognitive bias. We handled 28 female CD-1 mice for 16 weeks using either tail handling or cupped handling. The mice were then trained in an eight arm radial maze, where two adjacent arms predicted a positive outcome (darkness and food), while the two opposite arms predicted a negative outcome (no food, white noise and light). After six days of training, the mice were also given access to the four previously unavailable intermediate ambiguous arms of the radial maze and tested for cognitive bias. We were unable to validate this test, as mice from both handling groups displayed a similar pattern of exploration. Furthermore, we examined whether maze exploration is affected by the expression of stereotypic behaviour in the home cage. Mice with higher levels of stereotypic behaviour spent more time in positive arms and avoided ambiguous arms, displaying a negative cognitive bias. While this test needs further validation, our results indicate that it may allow the assessment of affective state in mice with minimal training-a major confound in current cognitive bias paradigms.

An Exploration Based Cognitive Bias Test for Mice: Effects of Handling Method and Stereotypic Behaviour

PubMed Central

Novak, Janja; Bailoo, Jeremy D.; Melotti, Luca; Rommen, Jonas; Würbel, Hanno

2015-01-01

Behavioural tests to assess affective states are widely used in human research and have recently been extended to animals. These tests assume that affective state influences cognitive processing, and that animals in a negative affective state interpret ambiguous information as expecting a negative outcome (displaying a negative cognitive bias). Most of these tests however, require long discrimination training. The aim of the study was to validate an exploration based cognitive bias test, using two different handling methods, as previous studies have shown that standard tail handling of mice increases physiological and behavioural measures of anxiety compared to cupped handling. Therefore, we hypothesised that tail handled mice would display a negative cognitive bias. We handled 28 female CD-1 mice for 16 weeks using either tail handling or cupped handling. The mice were then trained in an eight arm radial maze, where two adjacent arms predicted a positive outcome (darkness and food), while the two opposite arms predicted a negative outcome (no food, white noise and light). After six days of training, the mice were also given access to the four previously unavailable intermediate ambiguous arms of the radial maze and tested for cognitive bias. We were unable to validate this test, as mice from both handling groups displayed a similar pattern of exploration. Furthermore, we examined whether maze exploration is affected by the expression of stereotypic behaviour in the home cage. Mice with higher levels of stereotypic behaviour spent more time in positive arms and avoided ambiguous arms, displaying a negative cognitive bias. While this test needs further validation, our results indicate that it may allow the assessment of affective state in mice with minimal training—a major confound in current cognitive bias paradigms. PMID:26154309

Antidepressant-like Responses to Lithium in Genetically Diverse Mouse Strains

PubMed Central

Can, Adem; Blackwell, Robert A.; Piantadosi, Sean C.; Dao, David T.; O’Donnell, Kelley C.; Gould, Todd D.

2011-01-01

A mood stabilizing and antidepressant response to lithium is only found in a subgroup of bipolar disorder and depression patients. Identifying strains of mice that are responsive and non-responsive to lithium may elucidate genomic and other biological factors that play a role in lithium responsiveness. Mouse strains were tested in the forced swim, tail suspension, and open field tests after acute and chronic systemic, and intracerebroventricular and chronic lithium treatments. Serum and brain lithium levels were measured. Three (129S6/SvEvTac, C3H/HeNHsd, C57BL/6J) of the eight inbred strains tested, and one (CD-1) of the three outbred strains, showed an antidepressant-like response in the forced swim test following acute systemic administration of lithium. The three responsive inbred strains, as well as the DBA/2J strain, were also responsive in the forced swim test after chronic administration of lithium. However, in the tail suspension test, acute lithium resulted in an antidepressant-like effect only in C3H/HeNHsd mice. Only C57BL/6J and DBA/2J were responsive in the tail suspension test after chronic administration of lithium. Intracerebroventricular lithium administration resulted in a similar response profile in BALB/cJ (non-responsive) and C57BL/6J (responsive) strains. Serum and brain lithium concentrations demonstrated that behavioral results were not due to differential pharmacokinetics of lithium in individual strains, suggesting that genetic factors likely regulate responsiveness to lithium. Our results indicate that responsiveness to lithium in tests of antidepressant efficacy varies among genetically diverse mouse strains. These results will assist in identifying genomic factors associated with lithium responsiveness and the mechanisms of lithium action. PMID:21306560

Phytohormone abscisic acid elicits antinociceptive effects in rats through the activation of opioid and peroxisome proliferator-activated receptors β/δ.

PubMed

Mollashahi, Mahtab; Abbasnejad, Mehdi; Esmaeili-Mahani, Saeed

2018-08-05

The phytohormone abscisic acid exists in animal tissues particularly in the brain. However, its neurophysiological effects have not yet been fully clarified. This study was designed to evaluate the possible antinociceptive effects of abscisic acid on animal models of pain and determine its possible signaling mechanism. Tail-flick, hot-plate and formalin tests were used to assess the nociceptive threshold. All experiments were carried out on male Wistar rats. To determine the role of Peroxisome proliferator-activated receptor β/δ (PPARβ/δ) and opioid receptors on the induction of abscisic acid antinociception, specific antagonists were injected 15 min before abscisic acid. The data showed that abscisic acid (5, 10 and 15 µg/rat, i.c.v.) significantly decreased pain responses in formalin test. In addition, it could also produce dose-dependent antinociceptive effect in tail-flick and hot-plate tests. Administration of PPARβ/δ antagonist (GSK0660, 80 nM, i.c.v.) significantly attenuated the antinociceptive effect of abscisic acid in all tests. The antinociceptive effects of abscisic acid were completely inhibited by naloxone (6 µg, i.c.v.) during the time course of tail-flick and hot-plate tests. The results indicated that the central injection of abscisic acid has potent pain-relieving property which is mediated partly via the PPAR β/δ and opioid signaling. Copyright © 2018 Elsevier B.V. All rights reserved.

Quantitative traits for the tail suspension test: automation, optimization, and BXD RI mapping.

PubMed

Lad, Heena V; Liu, Lin; Payá-Cano, José L; Fernandes, Cathy; Schalkwyk, Leonard C

2007-07-01

Immobility in the tail suspension test (TST) is considered a model of despair in a stressful situation, and acute treatment with antidepressants reduces immobility. Inbred strains of mouse exhibit widely differing baseline levels of immobility in the TST and several quantitative trait loci (QTLs) have been nominated. The labor of manual scoring and various scoring criteria make obtaining robust data and comparisons across different laboratories problematic. Several studies have validated strain gauge and video analysis methods by comparison with manual scoring. We set out to find objective criteria for automated scoring parameters that maximize the biological information obtained, using a video tracking system on tapes of tail suspension tests of 24 lines of the BXD recombinant inbred panel and the progenitor strains C57BL/6J and DBA/2J. The maximum genetic effect size is captured using the highest time resolution and a low mobility threshold. Dissecting the trait further by comparing genetic association of multiple measures reveals good evidence for loci involved in immobility on chromosomes 4 and 15. These are best seen when using a high threshold for immobility, despite the overall better heritability at the lower threshold. A second trial of the test has greater duration of immobility and a completely different genetic profile. Frequency of mobility is also an independent phenotype, with a distal chromosome 1 locus.

Effect of agmatine on acute and mononeuropathic pain.

PubMed

Aricioglu, Feyza; Korcegez, Eylem; Bozkurt, Ayhan; Ozyalcin, Suleyman

2003-12-01

Agmatine is a polycationic amine synthesized from L-arginine by arginine decarboxylase in brain and several tissues. It binds to N-methyl-D-aspartate (NMDA) subtype of glutamatergic, alpha(2)-adrenergic and imidazoline (I) receptors. The present study was designed to investigate effect of agmatine on acute and mononeuropathic pain after chronic constriction injury (CCI). CCI was created by four loose ligations around the right sciatic nerve. The analgesic threshold in rats was evaluated by using thermal hyperalgesia/allodynia (THA) at 4 degrees C. The evaluations were made preoperatively, on postoperative day 15, and after drug administration. Agmatine (10, 20, 40, 80, and 100 mg/kg) was administered intraperitoneally for 5 days beginning on postoperative day 15. Agmatine significantly reduced the hyperalgesia in all doses applied. When agmatine was injected intraperitoneally (10, 20, 40, 80, and 100 mg/kg), it increased the nociceptive threshold in the tail-immersion test in a dose-dependent manner, but it had no effect in the hot-plate test. This effect of agmatine in the tail-immersion test was blocked by both yohimbine (1 mg/kg) and idazoxan (0.5 mg/kg). When agmatine was administered intracerebroventricularly (25-200 microg/10 microL), it increased the nociceptive threshold in the hot-plate but not in the tail-immersion test. We conclude that agmatine, an endogenous substance derived from arginine, can modulate both acute and chronic pain.

48. HISTORIC CLOSEUP VIEW OF THE REDSTONE ROCKET IN THE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

48. HISTORIC CLOSE-UP VIEW OF THE REDSTONE ROCKET IN THE TEST STAND, WITH THE TAIL SECTION REMOVED, REVEALING THE ROCKET ENGINE WITH SOME OF THE TESTING SENSORS ATTACHED. - Marshall Space Flight Center, Redstone Rocket (Missile) Test Stand, Dodd Road, Huntsville, Madison County, AL

A new data-based model of the global magnetospheric B-field: Modular structure, parameterization, first results.

NASA Astrophysics Data System (ADS)

Tsyganenko, Nikolai

2013-04-01

A new advanced model of the dynamical geomagnetosphere is presented, based on a large set of data from Geotail, Cluster, Polar, and Themis missions, taken during 138 storm events with SYM-H from -40 to -487nT over the period from 1996 through 2012 in the range of geocentric distances from ~3Re to ~60Re. The model magnetic field is confined within a realistic magnetopause, based on Lin et al. [JGRA, v.115, A04207, 2010] empirical boundary, driven by the dipole tilt angle, solar wind pressure, and IMF Bz. The magnetic field is modeled as a flexible combination of several modules, representing contributions from principal magnetospheric current systems such as the symmetric and partial ring currents (SRC/PRC), Region 1 and 2 field-aligned currents (FAC), and the equatorial tail current sheet (TCS). In the inner magnetosphere the model field is dominated by contributions from the SRC and PRC, derived from realistic particle pressure models and represented by four modules, providing variable degree of dawn-dusk and noon-midnight asymmetry. The TCS field is comprised of several independent modules, ensuring sufficient flexibility of the model field and correct asymptotic values in the distant tail. The Region 2 FAC is an inherent part of the PRC, derived from the continuity of the azimuthal current. The Region 1 FAC is modulated by the diurnal and seasonal variations of the dipole tilt angle, in agreement with earlier statistical studies [Ohtani et al., JGRA, v.110, A09230, 2005]. Following the approach introduced in our earlier TS05 model [Tsyganenko and Sitnov, JGRA, v.110, A03208, 2005], contributions from all individual field sources are parameterized by the external driving functions, derived from the solar wind/IMF OMNI database as solutions of dynamic equations with source and loss terms in the right-hand side. Global magnetic configurations and their evolution during magnetospheric storms are analyzed and discussed in context of the model results.

Initial Teacher Certification Testing in Massachusetts: A Case of the Tail Wagging the Dog.

ERIC Educational Resources Information Center

Flippo, Rona F.; Riccards, Michael P.

2000-01-01

An evaluation of the Massachusetts Educator Certification Test has revealed unforeseen, counterproductive consequences. Teacher preparation colleges are adjusting curricular emphases to teach to a test of dubious validity and are inadvertently excluding substantial portions of enrollees to boost test scores. Minorities are failing at higher rates.…

40 CFR 91.506 - Engine sample selection.

Code of Federal Regulations, 2010 CFR

2010-07-01

... paragraph (b)(2) of this section. It defines one-tail, 95 percent confidence intervals. σ=actual test sample... individual engine x=mean of emission test results of the actual sample FEL=Family Emission Limit n=The actual... carry-over engine families: After one engine is tested, the manufacturer will combine the test with the...

Dietary partitioning of Australia's two marsupial hypercarnivores, the Tasmanian devil and the spotted-tailed quoll, across their shared distributional range

PubMed Central

Johnson, Christopher N.; Barmuta, Leon A.; Jones, Menna E.

2017-01-01

Australia’s native marsupial fauna has just two primarily flesh-eating ‘hypercarnivores’, the Tasmanian devil (Sarcophilus harrisii) and the spotted-tailed quoll (Dasyurus maculatus) which coexist only on the island of Tasmania. Devil populations are currently declining due to a fatal transmissible cancer. Our aim was to analyse the diet of both species across their range in Tasmania, as a basis for understanding how devil decline might affect the abundance and distribution of quolls through release from competition. We used faecal analysis to describe diets of one or both species at 13 sites across Tasmania. We compared diet composition and breadth between the two species, and tested for geographic patterns in diets related to rainfall and devil population decline. Dietary items were classified into 6 broad categories: large mammals (≥ 7.0kg), medium-sized mammals (0.5–6.9kg), small mammals (< 0.5kg), birds, reptiles and invertebrates. Diet overlap based on prey-size category was high. Quoll diets were broader than devils at all but one site. Devils consumed more large and medium-sized mammals and quolls more small mammals, reptiles and invertebrates. Medium-sized mammals (mainly Tasmanian pademelon Thylogale billardierii), followed by large mammals (mainly Bennett’s wallaby Macropus rufogriseus) and birds, were the most important prey groups for both species. Diet composition varied across sites, suggesting that both species are flexible and opportunistic foragers, but was not related to rainfall for devils. Quolls included more large mammals but fewer small mammals and invertebrates in their diet in the eastern drier parts of Tasmania where devils have declined. This suggests that a competitive release of quolls may have occurred and the substantial decline of devils has provided more food in the large-mammal category for quolls, perhaps as increased scavenging opportunities. The high diet overlap suggests that if resources become limited in areas of high devil density, interspecific competition could occur. PMID:29176811

Effects of early human handling on the pain sensitivity of young lambs.

PubMed

Guesgen, Mirjam J; Beausoleil, Ngaio J; Stewart, Mairi

2013-01-01

Pain sensitivity of lambs changes over the first weeks of life. However, the effects of early treatments such as human handling on pain sensitivity are unknown for this species. This study investigated the effects of regular early gentle human handling on the pain sensitivity of lambs, indicated by their behavioural responses to tail docking. Prospective part-blinded experimental study. Twenty-nine singleton Coopworth lambs (females n=14, males n=15). Starting at one day of age, lambs were either handled twice daily for 2 weeks (Handled), were kept in the presence of lambs who were being handled but were not handled themselves (Presence), or were exposed to a human only during routine feeding and care (Control). At 3 weeks of age, all lambs were tail docked using rubber rings. Changes in behaviour due to docking were calculated and change data were analyzed using two-way anova with treatment and test pen as main factors. All lambs showed significant increases in the frequency and duration of behaviours indicative of pain, including 'abnormal' behaviours, and decreases in the frequency and duration of 'normal' behaviours after docking. Handled lambs showed a smaller increase in the time spent lying abnormally after docking than did Control lambs (mean transformed change in proportion of 30 minutes spent±SE: Control 0.55±0.04; Handled 0.38±0.03; Presence 0.48±0.03; C versus H t=3.45, p=0.007). These results provide some evidence that handling early in life may reduce subsequent pain sensitivity in lambs. While the behavioural effects of handling on pain behaviour were subtle, the results suggest, at the very least, that early handling does not increase pain sensitivity in lambs and suggests there is still flexibility postnatally in the pain processing system of a precocial species. © 2012 The Authors. Veterinary Anaesthesia and Analgesia. © 2012 Association of Veterinary Anaesthetists and the American College of Veterinary Anesthesiologists.

Comparative social grooming networks in captive chimpanzees and bonobos.

PubMed

Rodrigues, Michelle A; Boeving, Emily R

2018-06-21

Despite similar dispersal patterns, models of Pan sociality emphasize sex differences in social bonding between the two species. Such disparities are attributed to hypothesized differences in environmental selective pressures that structure association patterns. However, recent research documents greater within-species variation in social bonds in both species. Here, we examine grooming networks in captive chimpanzees at the North Carolina Zoo, and captive bonobos at the Columbus Zoo. We hypothesized that male-female grooming relationships would be the strongest in both species, but that males and females of both species would not significantly differ between centrality, strength, or clustering. Via Mantel tests, we found that neither bonobos (t = - 0.070, r = - 0.009, two-tailed p = 0.942) nor chimpanzees (t = - 0.495, r = - 0.0939, two-tailed p = 0.6205) had significant differences in grooming between or within sexes. Neither species had significant sex differences in centrality, strength, or clustering. To account for idiosyncratic factors affecting grooming distribution, we examined the effect of origin, kinship, and group tenure on social network position. We found that wild-born bonobos exhibited greater eigenvector centrality (t = - 2.592, df = 9, p = 0.29) and strength (t = - 2.401; df = 9, p = 0.040), and group tenure was significantly correlated with strength (r = 0.608; N = 11, p - 0 = 0.47). None of these factors varied with social network position in chimpanzees. Our findings suggest that in captive settings, idiosyncratic factors related to individual history play a greater role in structuring social networks. Such variation may point to the behavioral flexibility inherent in fission-fusion networks, and mirror between-site variation found in wild chimpanzees. However, some idiosyncratic factors shaping captive networks may be an artifact of captivity.

On the Generality of the "Sit and Reach" Test: An Analysis of Flexibility Data for an Aging Population.

ERIC Educational Resources Information Center

Shephard, Roy J.; And Others

1990-01-01

This study examined head rotation, shoulder extension and rotation, ankle plantar and dorsiflexion, hip flexion, and sit and reach (SR) in 80 adults, aged 45-75, to identify flexibility factors. No single measurement indicates loss of flexibility at all joints, but SR tests are found to be more reliable than others. (SM)

Biomechanical Analysis of Military Boots: Phase 2. Volume 1. Human User Testing of Military and Commercial Footwear

DTIC Science & Technology

1996-02-01

jungle boots, were subjected to tests of forefoot flexibility, rearfoot stability, outsole wear, water penetration, outsole friction, and impact...Testing of forefoot flexibility with the uppers in place revealed that the combat and the jungle boots were less flexible than all commercial items...began at the time of foot strike , or initial contact of the foot with the ground, and continued through toe-off, or termination of contact of the foot

Intervertebral disc adaptation to wedging deformation.

PubMed

Stokes, Ian A F; Aronsson, David D; Clark, Katherine C; Roemhildt, Maria L

2006-01-01

Although scoliosis includes wedge deformities of both vertebrae and discs, little is known about the causes of the discal changes, and whether they result from mechanical influences on growth and/or remodelling. An external apparatus attached to transvertebral pins applied compression and 15 degrees of angulation to each of two adjacent young rat caudal intervertebral discs for 5 weeks (four animals), or for 10 weeks (four animals). Each week, micro-CT scanning documented the in vivo discal wedging. After euthanasia, tail segments (three vertebrae and the 2 angulated discs) were excised and their flexibility was measured over a range of lateral bending. The angle of maximum flexibility was recorded. Then discs were fixed in situ (with the external apparatus in place) and sectioned for polarized light microscopy. The disc-wedging deformity averaged 15 degrees initially, it averaged 20 degrees after 5 weeks, and then reduced to 10 degrees (in 10 week animals). The lateral bending flexibility showed a distinct maximum at an average of 1.1 degrees from the in vivo position in the 5-week animals, indicating structural remodeling of the discs almost to the deformed geometry. The 10-week animals had maximum flexibility at 1.4 degrees from the in vivo position (no significant difference between 5 and 10-week animals.) Collagen crimp angles [Cassidy et al., Conn Tiss Res 1989, 23:75-88] were not significantly different between convex and concave sides, again suggesting that remodeling had occurred. In a mechanically induced scoliosis deformity in skeletally immature rats, the intervertebral discs underwent remodeling within 5 weeks. This indicates that this animal model is suitable for studying adaptive wedging changes in human scoliosis.

46 CFR 111.60-13 - Flexible electric cord and cables.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Flexible electric cord and cables. 111.60-13 Section 111... cables. (a) Construction and testing. Each flexible cord and cable must meet the requirements in section.... Each flexible cord must be No. 18 AWG (0.82 mm2) or larger. (e) Splices. Each flexible cord and cable...

Community-Supported Data Repositories in Paleobiology: A 'Middle Tail' Between the Geoscientific and Informatics Communities

NASA Astrophysics Data System (ADS)

Williams, J. W.; Ashworth, A. C.; Betancourt, J. L.; Bills, B.; Blois, J.; Booth, R.; Buckland, P.; Charles, D.; Curry, B. B.; Goring, S. J.; Davis, E.; Grimm, E. C.; Graham, R. W.; Smith, A. J.

2015-12-01

Community-supported data repositories (CSDRs) in paleoecology and paleoclimatology have a decades-long tradition and serve multiple critical scientific needs. CSDRs facilitate synthetic large-scale scientific research by providing open-access and curated data that employ community-supported metadata and data standards. CSDRs serve as a 'middle tail' or boundary organization between information scientists and the long-tail community of individual geoscientists collecting and analyzing paleoecological data. Over the past decades, a distributed network of CSDRs has emerged, each serving a particular suite of data and research communities, e.g. Neotoma Paleoecology Database, Paleobiology Database, International Tree Ring Database, NOAA NCEI for Paleoclimatology, Morphobank, iDigPaleo, and Integrated Earth Data Alliance. Recently, these groups have organized into a common Paleobiology Data Consortium dedicated to improving interoperability and sharing best practices and protocols. The Neotoma Paleoecology Database offers one example of an active and growing CSDR, designed to facilitate research into ecological and evolutionary dynamics during recent past global change. Neotoma combines a centralized database structure with distributed scientific governance via multiple virtual constituent data working groups. The Neotoma data model is flexible and can accommodate a variety of paleoecological proxies from many depositional contests. Data input into Neotoma is done by trained Data Stewards, drawn from their communities. Neotoma data can be searched, viewed, and returned to users through multiple interfaces, including the interactive Neotoma Explorer map interface, REST-ful Application Programming Interfaces (APIs), the neotoma R package, and the Tilia stratigraphic software. Neotoma is governed by geoscientists and provides community engagement through training workshops for data contributors, stewards, and users. Neotoma is engaged in the Paleobiological Data Consortium and other efforts to improve interoperability among cyberinfrastructure in the paleogeosciences.

Performance variation due to stiffness in a tuna-inspired flexible foil model.

PubMed

Rosic, Mariel-Luisa N; Thornycroft, Patrick J M; Feilich, Kara L; Lucas, Kelsey N; Lauder, George V

2017-01-17

Tuna are fast, economical swimmers in part due to their stiff, high aspect ratio caudal fins and streamlined bodies. Previous studies using passive caudal fin models have suggested that while high aspect ratio tail shapes such as a tuna's generally perform well, tail performance cannot be determined from shape alone. In this study, we analyzed the swimming performance of tuna-tail-shaped hydrofoils of a wide range of stiffnesses, heave amplitudes, and frequencies to determine how stiffness and kinematics affect multiple swimming performance parameters for a single foil shape. We then compared the foil models' kinematics with published data from a live swimming tuna to determine how well the hydrofoil models could mimic fish kinematics. Foil kinematics over a wide range of motion programs generally showed a minimum lateral displacement at the narrowest part of the foil, and, immediately anterior to that, a local area of large lateral body displacement. These two kinematic patterns may enhance thrust in foils of intermediate stiffness. Stiffness and kinematics exhibited subtle interacting effects on hydrodynamic efficiency, with no one stiffness maximizing both thrust and efficiency. Foils of intermediate stiffnesses typically had the greatest coefficients of thrust at the highest heave amplitudes and frequencies. The comparison of foil kinematics with tuna kinematics showed that tuna motion is better approximated by a zero angle of attack foil motion program than by programs that do not incorporate pitch. These results indicate that open questions in biomechanics may be well served by foil models, given appropriate choice of model characteristics and control programs. Accurate replication of biological movements will require refinement of motion control programs and physical models, including the creation of models of variable stiffness.

Improving value assessment of high-risk, high-reward biotechnology research: the role of 'thick tails'.

PubMed

Casault, Sébastien; Groen, Aard J; Linton, Jonathan D

2014-03-25

This paper presents work toward improving the efficacy of financial models that describe the unique nature of biotechnology firms. We show that using a 'thick tailed' power law distribution to describe the behavior of the value of biotechnology R&D used in a Real Options Pricing model is significantly more accurate than the traditionally used Gaussian approach. A study of 287 North-American biotechnology firms gives insights into common problems faced by investors, managers and other stakeholders when using traditional techniques to calculate the commercial value of R&D. This is important because specific quantitative tools to assess the value of high-risk, high-reward R&D do not currently exist. This often leads to an undervaluation of biotechnology R&D and R&D intensive biotechnology firms. For example, the widely used Net Present Value (NPV) method assumes a fixed risk ignoring management flexibility and the changing environment. However, Real Options Pricing models assume that commercial returns from R&D investments are described by a normal random walk. A normal random walk model eliminates the possibility of drastic changes to the marketplace resulting from the introduction of revolutionary products and/or services. It is possible to better understand and manage biotechnology research projects and portfolios using a model that more accurately considers large non-Gaussian price fluctuations with thick tails, which recognize the unusually large risks and opportunities associated with Biotechnology R&D. Our empirical data show that opportunity overcompensates for the downside risk making biotechnology R&D statistically more valuable than other Gaussian options investments, which may otherwise appear to offer a similar combination of risk and return. Copyright © 2013 Elsevier B.V. All rights reserved.

Nanoscopic dynamics of phospholipid in unilamellar vesicles: Effect of gel to fluid phase transition

DOE PAGES

Sharma, V. K.; Mamontov, E.; Anunciado, D. B.; ...

2015-03-04

Dynamics of phospholipids in unilamellar vesicles (ULV) is of interest in biology, medical, and food sciences since these molecules are widely used as biocompatible agents and a mimic of cell membrane systems. We have investigated the nanoscopic dynamics of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) phospholipid in ULV as a function of temperature using elastic and quasielastic neutron scattering (QENS). The dependence of the signal on the scattering momentum transfer, which is a critical advantage of neutron scattering techniques, allows the detailed analysis of the lipid motions that cannot be carried out by other means. In agreement with a differential scanning calorimetry measurement, amore » sharp rise in the elastic scattering intensity below ca. 296 K indicates a phase transition from the high-temperature fluid phase to the low-temperature solid gel phase. The microscopic lipid dynamics exhibits qualitative differences between the solid gel phase (in a measurement at 280 K) and the fluid phase (in a measurement at a physiological temperature of 310 K). The data analysis invariably shows the presence of two distinct motions: the whole lipid molecule motion within a monolayer, or lateral diffusion, and the relatively faster internal motion of the DMPC molecule. The lateral diffusion of the whole lipid molecule is found to be Fickian in character, whereas the internal lipid motions are of localized character, consistent with the structure of the vesicles. The lateral motion slows down by an order of magnitude in the solid gel phase, whereas for the internal motion not only the time scale, but also the character of the motion changes upon the phase transition. In the solid gel phase, the lipids are more ordered and undergo uniaxial rotational motion. However, in the fluid phase, the hydrogen atoms of the lipid tails undergo confined translation diffusion rather than uniaxial rotational diffusion. The localized translational diffusion of the hydrogen atoms of the lipid tails is a manifestation of the flexibility of the chains acquired in the fluid phase. Because of this flexibility, both the local diffusivity and the confinement volume for the hydrogen atoms increase linearly from near the lipid s polar head group to the end of its hydrophobic tail. Our results present a quantitative and detailed picture of the effect of the gel-fluid phase transition on the nanoscopic lipid dynamics in ULV. Lastly, the data analysis approach developed here has a potential for probing the dynamic response of lipids to the presence of additional cell membrane components.« less

Synergism between COX-3 inhibitors in two animal models of pain.

PubMed

Muñoz, J; Navarro, C; Noriega, V; Pinardi, G; Sierralta, F; Prieto, J C; Miranda, H F

2010-04-01

The antinociception induced by the intraperitoneal coadministration in mice of combinations of metamizol and paracetamol was evaluated in the tail flick test and orofacial formalin test. The antinociception of each drugs alone and the interaction of the combinations was evaluated by isobolographic analysis in the tail-flick and in the formalin orofacial assay of mice. Mice pretreated with the drugs demonstrated that the antinociception of metamizol and paracetamol is dose-dependent. The potency range on the antinocifensive responses for metamizol or paracetamol was as follows: orofacial (Phase II) > orofacial (Phase I) > tail flick. In addition, the coadministration of metamizol with paracetamol induced a strong synergistic antinociception in the algesiometer assays. Both drugs showed effectiveness in inflammatory pain. These actions can be related to the differential selectivity of the drugs for inhibition of COX isoforms and also to the several additional antinociception mechanisms and pathways initiated by the analgesic drugs on pain transmission. Since the efficacy of the combination of metamizol with paracetamol has been demonstrated in the present study, this association could have a potential beneficial effect on the pharmacological treatment of clinical pain.

MPLM On-Orbit Interface Dynamic Flexibility Modal Test

NASA Technical Reports Server (NTRS)

Bookout, Paul S.; Rodriguez, Pedro I.; Tinson, Ian; Fleming, Paolo

2001-01-01

Now that the International Space Station (ISS) is being constructed, payload developers have to not only verify the Shuttle-to-payload interface, but also the interfaces their payload will have with the ISS. The Multi Purpose Logistic Module (MPLM) being designed and built by Alenia Spazio in Torino, Italy is one such payload. The MPLM is the primary carrier for the ISS Payload Racks, Re-supply Stowage Racks, and the Resupply Stowage Platforms to re-supply the ISS with food, water, experiments, maintenance equipment and etc. During the development of the MPLM there was no requirement for verification of the on-orbit interfaces with the ISS. When this oversight was discovered, all the dynamic test stands had already been disassembled. A method was needed that would not require an extensive testing stand and could be completed in a short amount of time. The residual flexibility testing technique was chosen. The residual flexibility modal testing method consists of measuring the free-free natural frequencies and mode shapes along with the interface frequency response functions (FRF's). Analytically, the residual flexibility method has been investigated in detail by, MacNeal, Martinez, Carne, and Miller, and Rubin, but has not been implemented extensively for model correlation due to difficulties in data acquisition. In recent years improvement of data acquisition equipment has made possible the implementation of the residual flexibility method as in Admire, Tinker, and Ivey, and Klosterman and Lemon. The residual flexibility modal testing technique is applicable to a structure with distinct points (DOF) of contact with its environment, such as the MPLM-to-Station interface through the Common Berthing Mechanism (CBM). The CBM is bolted to a flange on the forward cone of the MPLM. During the fixed base test (to verify Shuttle interfaces) some data was gathered on the forward cone panels. Even though there was some data on the forward cones, an additional modal test was performed to better characterize its behavior. The CBM mounting flange is the only remaining structure of the MPLM that no test data was available. This paper discusses the implementation of the residual flexibility modal testing technique on the CBM flange and the modal test of the forward cone panels.