Sample records for tamarind wood activated

  1. Optimization of production conditions for activated carbons from Tamarind wood by zinc chloride using response surface methodology.

    PubMed

    Sahu, J N; Acharya, Jyotikusum; Meikap, B C

    2010-03-01

    The low-cost activated carbon was prepared from Tamarind wood an agricultural waste material, by chemical activation with zinc chloride. Activated carbon adsorption is an effective means for reducing organic chemicals, chlorine, heavy metals and unpleasant tastes and odours in effluent or colored substances from gas or liquid streams. Central composite design (CCD) was applied to study the influence of activation temperature, chemical ratio of zinc chloride to Tamarind wood and activation time on the chemical activation process of Tamarind wood. Two quadratic models were developed for yield of activated carbon and adsorption of malachite green oxalate using Design-Expert software. The models were used to calculate the optimum operating conditions for production of activated carbon providing a compromise between yield and adsorption of the process. The yield (45.26 wt.%) and adsorption (99.9%) of the activated carbon produced at these operating conditions showed an excellent agreement with the amounts predicted by the models. Copyright (c) 2009 Elsevier Ltd. All rights reserved.

  2. Characterization and Properties of Activated Carbon Prepared from Tamarind Seeds by KOH Activation for Fe(III) Adsorption from Aqueous Solution

    PubMed Central

    Mopoung, Sumrit; Moonsri, Phansiri; Palas, Wanwimon; Khumpai, Sataporn

    2015-01-01

    This research studies the characterization of activated carbon from tamarind seed with KOH activation. The effects of 0.5 : 1–1.5 : 1 KOH : tamarind seed charcoal ratios and 500–700°C activation temperatures were studied. FTIR, SEM-EDS, XRD, and BET were used to characterize tamarind seed and the activated carbon prepared from them. Proximate analysis, percent yield, iodine number, methylene blue number, and preliminary test of Fe(III) adsorption were also studied. Fe(III) adsorption was carried out by 30 mL column with 5–20 ppm Fe(III) initial concentrations. The percent yield of activated carbon prepared from tamarind seed with KOH activation decreased with increasing activation temperature and impregnation ratios, which were in the range from 54.09 to 82.03 wt%. The surface functional groups of activated carbon are O–H, C=O, C–O, –CO3, C–H, and Si–H. The XRD result showed high crystallinity coming from a potassium compound in the activated carbon. The main elements found in the activated carbon by EDS are C, O, Si, and K. The results of iodine and methylene blue adsorption indicate that the pore size of the activated carbon is mostly in the range of mesopore and macropore. The average BET pore size and BET surface area of activated carbon are 67.9764 Å and 2.7167 m2/g, respectively. Finally, the tamarind seed based activated carbon produced with 500°C activation temperature and 1.0 : 1 KOH : tamarind seed charcoal ratio was used for Fe(III) adsorption test. It was shown that Fe(III) was adsorbed in alkaline conditions and adsorption increased with increasing Fe(III) initial concentration from 5 to 20 ppm with capacity adsorption of 0.0069–0.019 mg/g. PMID:26689357

  3. Key volatile aroma compounds of three black velvet tamarind (Dialium) fruit species.

    PubMed

    Lasekan, Ola; See, Ng Siew

    2015-02-01

    Nineteen odour-active compounds were quantified in three black velvet tamarind fruit species. Calculation of the odour activity values (OAVs) of the odorants showed that differences in odour profiles of the tamarinds were mainly caused by linalool, limonene, 4-hydroxy-2,5-dimethyl-3(2H)-furanone, nonanal, and (Z)-3-hexenal. On the basis of their high OAVs, cis-linalool oxide (furanoid), geranyl acetone, and cinnamyl acetate were identified as other potent odorants in the three tamarinds. Sensory studies revealed very distinct aroma profiles, which are characteristic of these types of fruits. While the Dialiumguineense elicited floral, flowery, caramel-like notes, the other two species were dominated by leaf-like, caramel, and green notes. Copyright © 2014 Elsevier Ltd. All rights reserved.

  4. Synthesis, characterization and evaluation of thiolated tamarind seed polysaccharide as a mucoadhesive polymer.

    PubMed

    Kaur, Harmanmeet; Yadav, Shikha; Ahuja, Munish; Dilbaghi, Neeraj

    2012-11-06

    In the present study, thiol-functionalization of tamarind seed polysaccharide was carried out by esterification with thioglycolic acid. Thiol-functionalization was confirmed by SH stretch in Fourier-transformed infra-red spectra at 2586 cm(-1). It was found to possess 104.5 mM of thiol groups per gram. The results of differential scanning calorimetry and X-ray diffraction study indicate increase in crystallinity. Polymer compacts of thiolated tamarind seed polysaccharide required 6.85-fold greater force to detach from the mucin coated membrane than that of tamarind seed polysaccharide. Comparative evaluation of Carbopol-based metronidazole gels containing thiolated tamarind seed polysaccharide with gels containing tamarind seed polysaccharide for mucoadhesive strength using chicken ileum by modified balance method revealed higher mucoadhesion of gels containing thiolated tamarind seed polysaccharide. Further, the gels containing tamarind seed polysaccharide and thiolated tamarind seed polysaccharide released the drug by Fickian-diffusion following the first-order and Higuchi's-square root release kinetics, respectively. Copyright © 2012 Elsevier Ltd. All rights reserved.

  5. 21 CFR 176.350 - Tamarind seed kernel powder.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 3 2014-04-01 2014-04-01 false Tamarind seed kernel powder. 176.350 Section 176... Paperboard § 176.350 Tamarind seed kernel powder. Tamarind seed kernel powder may be safely used as a component of articles intended for use in producing, manufacturing, packing, processing, preparing, treating...

  6. 21 CFR 176.350 - Tamarind seed kernel powder.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 3 2011-04-01 2011-04-01 false Tamarind seed kernel powder. 176.350 Section 176... Substances for Use Only as Components of Paper and Paperboard § 176.350 Tamarind seed kernel powder. Tamarind seed kernel powder may be safely used as a component of articles intended for use in producing...

  7. 21 CFR 176.350 - Tamarind seed kernel powder.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 3 2012-04-01 2012-04-01 false Tamarind seed kernel powder. 176.350 Section 176... Substances for Use Only as Components of Paper and Paperboard § 176.350 Tamarind seed kernel powder. Tamarind seed kernel powder may be safely used as a component of articles intended for use in producing...

  8. 21 CFR 176.350 - Tamarind seed kernel powder.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Tamarind seed kernel powder. 176.350 Section 176... Substances for Use Only as Components of Paper and Paperboard § 176.350 Tamarind seed kernel powder. Tamarind seed kernel powder may be safely used as a component of articles intended for use in producing...

  9. 21 CFR 176.350 - Tamarind seed kernel powder.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Tamarind seed kernel powder. 176.350 Section 176... Substances for Use Only as Components of Paper and Paperboard § 176.350 Tamarind seed kernel powder. Tamarind seed kernel powder may be safely used as a component of articles intended for use in producing...

  10. Production and characterization of cellulose reinforced starch (CRT) films.

    PubMed

    Sudharsan, K; Chandra Mohan, C; Azhagu Saravana Babu, P; Archana, G; Sabina, K; Sivarajan, M; Sukumar, M

    2016-02-01

    Starch from Tamarind seed is considered to be a nonedible and inexpensive component, with many industrial applications. Extraction and characterization of tamarind seed starch was carried out for the synthesis of biopolymer. Tamarind seeds were collected, cleaned and further roasted, decorticated, and pulverized to get starch powder. Total starch content present in each tamarind seed is estimated to be around 65-70%. About 84.68% purified starch can be recovered from the tamarind seed. Defatted Tamarind seed starch has an amylose content of 27.55 wt.% and 72.45 wt.% of amylopectin. Morphological (SEM) and X-ray diffraction were used to evaluate crystallinity. Likewise, TGA and DSC of starch have also been analyzed. Thermal properties of starch obtained from tamarind seeds showed good thermal stability when compared to other starch sources such as Mesquite seed and Mango kernel. This study proved that the tamarind seed starch can be used as a potential biopolymer material. Thermo-stable biofilms were produced through initial optimization studies. Predictive response surface quadratic models were constructed for prediction and optimization of biofilm mechanical properties. Correlation coefficient values were calculated to me more than 0.90 for mechanical responses which implies the fitness of constructed model with experimental data. Copyright © 2015 Elsevier B.V. All rights reserved.

  11. Effects of sodium benzoate on storage stability of previously improved beverage from tamarind (Tamarindus indica L.).

    PubMed

    Adeola, Abiodun A; Aworh, Ogugua C

    2014-01-01

    The effect of sodium benzoate on the quality attributes of improved tamarind beverage during storage was investigated. Tamarind beverages were produced according to a previously reported improved method, with or without chemical preservatives (100 mg/100 mL sodium benzoate). Tamarind beverage produced according to traditional processing method served as the control. The tamarind beverages were stored for 4 months at room (29 ± 2°C) and refrigerated (4-10°C) temperatures. Samples were analyzed, at regular intervals, for chemical, sensory, and microbiological qualities. Appearance of coliforms or overall acceptability score of 5.9 was used as deterioration index. The control beverages deteriorated by 2nd and 10th days at room and refrigerated temperatures, respectively. Improved tamarind beverage produced without the inclusion of sodium benzoate was stable for 3 and 5 weeks at room and refrigerated temperatures, respectively. Sodium benzoate extended the shelf life of the improved tamarind beverage to 6 and 13 weeks, respectively, at room and refrigerated temperatures.

  12. Bioactive characteristics and optimization of tamarind seed protein hydrolysate for antioxidant-rich food formulations.

    PubMed

    Bagul, Mayuri B; Sonawane, Sachin K; Arya, Shalini S

    2018-04-01

    Tamarind seed has been a source of valuable nutrients such as protein (contains high amount of many essential amino acids), essential fatty acids, and minerals which are recognized as additive to develop perfect balanced functional foods. The objective of present work was to optimize the process parameters for extraction and hydrolysis of protein from tamarind seeds. Papain-derived hydrolysates showed a maximum degree of hydrolysis (39.49%) and radical scavenging activity (42.92 ± 2.83%) at optimized conditions such as enzyme-to-substrate ratio (1:5), hydrolysis time (3 h), hydrolysis temperature (65 °C), and pH 6. From this study, papain hydrolysate can be considered as good source of natural antioxidants in developing food formulations.

  13. Sensory properties and consumer acceptance of sweet tamarind varieties grown in Thailand.

    PubMed

    Oupadissakoon, Chintana; Chambers, Edgar; Kongpensook, Varapha; Suwonsichon, Suntaree; Yenket, Renoo; Retiveau, Annlyse

    2010-04-30

    Sweet tamarind is a major edible fruit and flavoring ingredient particularly in south-east and southern Asia. Little research has focused on the fruit and almost nothing is known of its particular sensory properties. The aims of this research were to develop a lexicon for describing sweet tamarind, to compare varieties grown in Thailand, determine if orchard impacts sensory properties, and determine consumer acceptance of the varieties. A descriptive sensory lexicon of 25 terms was developed and six varieties were grouped into three clusters based on their sensory properties. The clusters appear to represent varieties that differ in their dark fruity notes and firm, fibrous texture. Generally, the orchard in which the plants were grown had little effect on sensory properties. In general, Sithong was liked by consumers along with Kunthee and Pragaithong. Intapalum was liked less but one small segment of consumers disliked Sithong and liked the Intapalum variety more. This research provides a foundation for further sensory and consumer research on sweet tamarind varieties by providing the initial data on the sensory properties of sweet tamarind, a lexicon that can be used for future research, and information on the consumer acceptance of tamarind varieties.

  14. Six-Month Chronic Toxicity Study of Tamarind Pulp (Tamarindus indica L.) Water Extract.

    PubMed

    Iskandar, Irene; Setiawan, Finna; Sasongko, Lucy D N; Adnyana, I Ketut

    2017-03-08

    Tamarind water extract has been shown to demonstrate an anti-obesity effect. In this research, long-term use of tamarind pulp water extract safety was evaluated. Tamarind pulp was extracted by reflux method, followed by freeze-drying to obtain dry extract. Wistar rats were divided into six groups, with 20 animals of each sex per group. The control group and satellite control group received carboxymethylcellulose sodium (CMC-Na) 0.5% 1 mL/100 g bw (body weight) per day. Treatment groups received tamarind pulp extract at doses of 75, 200, 1000, satellite 1000 mg/kg bw per day for six months. After six months, control groups and the treatment group were sacrificed. Satellite groups were sacrificed one month later. Relative organ weights, hematology and clinical biochemistry profiles were determined. After six months, there were no significant change in body weight, hematologic, and clinical biochemistry profiles of the tested group. Body weight of male rats in the satellite 1000 mg/kg bw group was significantly increased in week 30 compared to the satellite control group ( p < 0.05). The relative spleen weight of female rats of the 200 mg/kg bw group was reduced ( p < 0.05). The relative kidney weight of male rats in the 1000 mg/kg bw group was increased ( p < 0.05). This study showed that tamarind pulp extract was generally safe and well tolerated at the tested dose.

  15. Brazilian fruit pulps as functional foods and additives: evaluation of bioactive compounds.

    PubMed

    Paz, Mário; Gúllon, Patricia; Barroso, M Fátima; Carvalho, Ana P; Domingues, Valentina F; Gomes, Ana M; Becker, Helena; Longhinotti, Elisane; Delerue-Matos, Cristina

    2015-04-01

    Eight tropical fruit pulps from Brazil were simultaneously characterised in terms of their antioxidant and antimicrobial properties. Antioxidant activity was screened by DPPH radical scavenging activity (126-3987 mg TE/100g DW) and ferric reduction activity power (368-20819 mg AAE/100g DW), and complemented with total phenolic content (329-12466 mg GAE/100g DW) and total flavonoid content measurements (46-672 mg EE /100g DW), whereas antimicrobial activity was tested against the most frequently found food pathogens. Acerola and açaí presented the highest values for the antioxidant-related measurements. Direct correlations between these measurements could be observed for some of the fruits. Tamarind exhibited the broadest antimicrobial potential, having revealed growth inhibition of Pseudomonas aeruginosa. Escherichia coli, Listeria monocytogenes, Salmonella sp. and Staphylococcus aureus. Açaí and tamarind extracts presented an inverse relationship between antibacterial and antioxidant activities, and therefore, the antibacterial activity cannot be attributed (only) to phenolic compounds. Copyright © 2014 Elsevier Ltd. All rights reserved.

  16. Development of Wax-Incorporated Emulsion Gel Beads for the Encapsulation and Intragastric Floating Delivery of the Active Antioxidant from Tamarindus indica L.

    PubMed

    Soradech, Sitthiphong; Petchtubtim, Intira; Thongdon-A, Jeerayu; Muangman, Thanchanok

    2016-03-22

    In this study, tamarind (Tamarindus indica L.) seed extracts with potential antioxidant activity and toxicity to cancer cells were developed as functional foods and nutraceutical ingredients in the form of emulsion gel beads. Three extracts were obtained from ethanol and water: TSCH50, TSCH95 and TSCH. All extracts exhibited high potential for superoxide anion scavenging activity over the IC50 range < 5-11 µg/mL and had no toxic effects on normal cells, however, the water extract (TSCH) was the most effective due to its free radical scavenging activity and toxicity in mitochondrial membranes of cancer cells. Next a study was designed to develop a new formulation for encapsulation and intragastric floating delivery of tamarind seed extract (TSCH) using wax-incorporated emulsion gel beads, which were prepared using a modified ionotropic gelation technique. Tamarind seed extract at 1% (w/w) was used as the active ingredient in all formulations. The effect of the types and amounts of wax on the encapsulation efficiency and percentage of the active release of alginate gel beads was also investigated. The results demonstrated that the incorporation of both waxes into the gel beads had an effect on the percentage of encapsulation efficiency (%) and the percentage of the active ingredient release. Furthermore, the addition of water insoluble waxes (carnauba and bee wax) significantly retarded the release of the active ingredient. The addition of both waxes had a slight effect on drug release behavior. Nevertheless, the increase in incorporated waxes in all formulations could sustain the percentage of active ingredient release. In conclusion, wax-incorporated emulsion gel beads using a modified ionotropic gelation technique could be applied for the intragastric floating delivery and controlled release of functional food and nutraceutical products for their antioxidant and anticancer capacity.

  17. Microbial quality of yellow seasoned “pindang” fish treated with turmeric and tamarind

    NASA Astrophysics Data System (ADS)

    Handayani, B. R.; Dipokusumo, B.; Werdiningsih, W.; Rahayu, T. I.; Sugita, D. L.

    2018-01-01

    The objective of this study was to determine the microbial quality of yellow seasoned pindang fish. The fish was treated using combination of turmeric and tamarind at different ratio. This research used Randomized Block Design with 2 (two) factors ie concentration of turmeric (0%, 2%, and 6%) and concentration of tamarind (0%, 3%, and 6%). Each treatment was replicated 3 times to obtain 27 experimental units. The parameters observed were total microbe, total fungi and some pathogenic bacteria. Some microbial data were analyzed using descriptive method, however, the number of S. aureus was analyzed at 5% significance level by using software co-Stat and if there was a real difference then tested further by test Honestly Significant Difference (HSD). The results showed that increasing the use of curcumin and tamarind tended to decrease the total number of microbial from treatment control 5.1 x 105 CFU/gram to <1.0 x 103 CFU/gram. All the treatment produced yellow seasoned pindang fish with fungi <1.0 x 102 CFU/gram. The products contain pathogenic bacteria E. coli < 3 MPN/gram; S. aureus <1.0 x 103 CFU/gram; Salmonella and V. cholerae were negative in 25 gram of sample. Based on microbial quality, it is recommended that the use of 3-6% of turmeric and 2-4% of tamarind are the best spices combination to produce safe consumption of yellow seasoned pindang fish.

  18. Lead-contaminated imported tamarind candy and children's blood lead levels.

    PubMed

    Lynch, R A; Boatright, D T; Moss, S K

    2000-01-01

    In 1999, an investigation implicated tamarind candy as the potential source of lead exposure for a child with a significantly elevated blood lead level (BLL). The Oklahoma City-County Health Department tested two types of tamarind suckers and their packaging for lead content. More than 50% of the tested suckers exceeded the US Food and Drug Administration (FDA) Level of Concern for lead in this type of product. The authors calculated that a child consuming one-quarter to one-half of either of the two types of suckers in a day would exceed the maximum FDA Provis onal Tolerable Intake for lead. High lead concentrations in the two types of wrappers suggested leaching as a potential source of contamination. The authors used the Environmental Protection Agency's Integrated Exposure Uptake Biokinetic (IEUBK) model to predict the effects of consumption of contaminated tamarind suckers on populat on BLLs. The IEUBK model predicted that consumption of either type of sucker at a rate of one per day would result in dramatic increases in mean BLLs for children ages 6-84 months in Oklahoma and in the percentage of children wth elevated BLLs (> or =10 micrograms per deciliter [microg/dL]). The authors conclude that consumption of these products represents a potential public health threat. In addition, a history of lead contamination in imported tamarind products suggests that import control measures may not be completely effective in preventing additional lead exposure.

  19. Development of Suppositories Containing Flutamide-Loaded Alginate-Tamarind Microparticles for Rectal Administration: In Vitro and in Vivo Studies.

    PubMed

    Patil, Bharati Shivajirao; Mahajan, Hitendra Shaligram; Surana, Sanjay Javerilal

    2015-01-01

    In the present work the absorption of flutamide from suppositories containing hydrophilic tamarind alginate microparticles after rectal administration in rats was investigated with the purpose of enhancing bioavailability and to avoid hepatic toxicity. Microparticles were developed by ionic gelation method and optimized using one factorial design of response surface methodology. The optimized batch of microparticles had tamarind gum-sodium alginate (1 : 3) ratio and showed entrapment efficiency 94.969% and mucoadhesion strength 94.646% with desirability of 0.961. Suppositories loaded with microparticles were developed by fusion method using poloxamer 407 and poloxamer 188 in combination as suppository base. Kinetic analysis of the release data of microparticle-loaded suppositories showed time-independent release of drug. Higher values of 'n' (>0.89) represent Super Case II-type drug release. The pharmacokinetics of flutamide from flutamide tamarind alginate microparticle-loaded suppository were compared with oral suspension. Cmax of microparticle-loaded suppository was significantly larger than that of oral suspension (1.711 and 0.859 µg/mL, respectively).

  20. Lead-contaminated imported tamarind candy and children's blood lead levels.

    PubMed Central

    Lynch, R A; Boatright, D T; Moss, S K

    2000-01-01

    In 1999, an investigation implicated tamarind candy as the potential source of lead exposure for a child with a significantly elevated blood lead level (BLL). The Oklahoma City-County Health Department tested two types of tamarind suckers and their packaging for lead content. More than 50% of the tested suckers exceeded the US Food and Drug Administration (FDA) Level of Concern for lead in this type of product. The authors calculated that a child consuming one-quarter to one-half of either of the two types of suckers in a day would exceed the maximum FDA Provis onal Tolerable Intake for lead. High lead concentrations in the two types of wrappers suggested leaching as a potential source of contamination. The authors used the Environmental Protection Agency's Integrated Exposure Uptake Biokinetic (IEUBK) model to predict the effects of consumption of contaminated tamarind suckers on populat on BLLs. The IEUBK model predicted that consumption of either type of sucker at a rate of one per day would result in dramatic increases in mean BLLs for children ages 6-84 months in Oklahoma and in the percentage of children wth elevated BLLs (> or =10 micrograms per deciliter [microg/dL]). The authors conclude that consumption of these products represents a potential public health threat. In addition, a history of lead contamination in imported tamarind products suggests that import control measures may not be completely effective in preventing additional lead exposure. PMID:11354337

  1. Effects of bilimbi (Averrhoa bilimbi L.) and tamarind (Tamarindus indica L.) juice on Listeria monocytogenes Scott A and Salmonella Typhimurium ATCC 14028 and the sensory properties of raw shrimps.

    PubMed

    Norhana, M N Wan; Azman, Mohd Nor A; Poole, Susan E; Deeth, Hilton C; Dykes, Gary A

    2009-11-30

    The potential of using juice of bilimbi (Averrhoa bilimbi L.) and tamarind (Tamarindus indica L.) to reduce Listeria monocytogenes Scott A and Salmonella Typhimurium ATCC 14028 populations on raw shrimps after washing and during storage (4 degrees C) was investigated. The uninoculated raw shrimps and those inoculated with approximately 9 log cfu/ml of L. monocytogenes Scott A and S. Typhimurium ATCC 14028 were washed (dipped or rubbed) in distilled water (SDW) (control), bilimbi or tamarind juice at 1:4 (w/v) concentrations for 10 and 5 min. Naturally occurring aerobic bacteria (APC), L. monocytogenes Scott A and S. Typhimurium ATCC 14028 counts, pH values and sensory analysis of washed shrimps were determined immediately after washing (day 0), and on days 3 and 7 of storage. Compared to SDW, bilimbi and tamarind juice significantly (p<0.05) reduced APC (0.40-0.70 log cfu/g), L. monocytogenes Scott A (0.84-1.58 log cfu/g) and S. Typhimurium ATCC 14028 (1.03-2.00 log cfu/g) populations immediately after washing (0 day). There was a significant difference (p<0.05) in bacterial reduction between the dipping (0.40-0.41 log for APC; 0.84 for L. monocytogenes Scott A and 1.03-1.09 log for S. Typhimurium ATCC 14028) and rubbing (0.68-0.70 log for APC; 1.34-1.58 for L. monocytogenes Scott A and 1.67-2.00 log for S. Typhimurium ATCC 14028) methods. Regardless of washing treatments or methods, populations of S. Typhimurium ATCC 14028 decreased slightly (5.10-6.29 log cfu/g on day 7 of storage) while populations of L. monocytogenes Scott A (8.74-9.20 log cfu/g) and APC (8.68-8.92 log cfu/g) increased significantly during refrigerated storage. The pH of experimental shrimps were significantly (p<0.05) decreased by 0.15-0.22 pH units after washing with bilimbi and tamarind juice. The control, bilimbi or tamarind-washed shrimps did not differ in sensory panellist acceptability (p>0.05) throughout the storage except for odour (p<0.05) attributes at 0 day when acidic or lemony smell was noticed in bilimbi- and tamarind-washed shrimps and not in control shrimps.

  2. Preparation of tamarind fruit seed activated carbon by microwave heating for the adsorptive treatment of landfill leachate: A laboratory column evaluation.

    PubMed

    Foo, K Y; Lee, L K; Hameed, B H

    2013-04-01

    The preparation of tamarind fruit seed granular activated carbon (TSAC) by microwave induced chemical activation for the adsorptive treatment of semi-aerobic landfill leachate has been attempted. The chemical and physical properties of TSAC were examined. A series of column tests were performed to determine the breakthrough characteristics, by varying the operational parameters, hydraulic loading rate (5-20 mL/min) and adsorbent bed height (15-21 cm). Ammonical nitrogen and chemical oxygen demand (COD), which provide a prerequisite insight into the prediction of leachate quality was quantified. Results illustrated an encouraging performance for the adsorptive removal of ammonical nitrogen and COD, with the highest bed capacity of 84.69 and 55.09 mg/g respectively, at the hydraulic loading rate of 5 mL/min and adsorbent bed height of 21 cm. The dynamic adsorption behavior was satisfactory described by the Thomas and Yoon-Nelson models. The findings demonstrated the applicability of TSAC for the adsorptive treatment of landfill leachate. Copyright © 2013 Elsevier Ltd. All rights reserved.

  3. High performance solid-state electric double layer capacitor from redox mediated gel polymer electrolyte and renewable tamarind fruit shell derived porous carbon.

    PubMed

    Senthilkumar, S T; Selvan, R Kalai; Melo, J S; Sanjeeviraja, C

    2013-11-13

    The activated carbon was derived from tamarind fruit shell and utilized as electrodes in a solid state electrochemical double layer capacitor (SSEDLC). The fabricated SSEDLC with PVA (polyvinyl alcohol)/H2SO4 gel electrolyte delivered high specific capacitance and energy density of 412 F g(-1) and 9.166 W h kg(-1), respectively, at 1.56 A g(-1). Subsequently, Na2MoO4 (sodium molybdate) added PVA/H2SO4 gel electrolyte was also prepared and applied for SSEDLC, to improve the performance. Surprisingly, 57.2% of specific capacitance (648 F g(-1)) and of energy density (14.4 Wh kg(-1)) was increased while introducing Na2MoO4 as the redox mediator in PVA/H2SO4 gel electrolyte. This improved performance is owed to the redox reaction between Mo(VI)/Mo(V) and Mo(VI)/Mo(IV) redox couples in Na2MoO4/PVA/H2SO4 gel electrolyte. Similarly, the fabricated device shows the excellent capacitance retention of 93% for over 3000 cycles. The present work suggests that the Na2MoO4 added PVA/H2SO4 gel is a potential electrolyte to improve the performance instead of pristine PVA/H2SO4 gel electrolyte. Based on the overall performance, it is strongly believed that the combination of tamarind fruit shell derived activated carbon and Na2MoO4/PVA/H2SO4 gel electrolyte is more attractive in the near future for high performance SSEDLCs.

  4. Feasibility study and sensory test of turmeric tamarind traditional drink with various processing

    NASA Astrophysics Data System (ADS)

    Rudyatmi, E.; Bintari, S. H.; Iswari, R. S.

    2018-03-01

    Turmeric tamarind traditional drink as anti-oxidant, good for health, and has a potency to developed. The objectives of this study are to determine the feasibility and the favorite level of consumer towards two turmerics tamarind traditional drink which processed differently. Feasibility depended on sensory test and microbe's contamination according to a quality standard of BPOM and complemented with an anti-bacterial test. The anti-bacterial test is determined by a diameter of inhibition zone towards E coli and B subtilist. Consumer’s favorites level is determined by organoleptic test to 40 testers. Sensory test results to form, odor, taste, and color are normal. TPC first traditional drink 6,9 x 102 col/gr and TKK ≤1,0x10 col/gr, TPC second traditional drink 2,0 x 101 col/gr and TKK ≤1,0x10 col/gr; all tests toward E coli, Salmonella SP, Staphylococcus aureus, Pseudomonas aeruginosa, shigella sp negative/gr; inhibiting capabilities towards B.subtilis and E coli of first traditional drink > second traditional drink. Inhibiting capabilities towards B.subtilis > E coli. All parameters comply with BPOM standard. Most of the tester love the first method turmeric tamarind traditional drink.

  5. Screening of polysaccharides from tamarind, fenugreek and jackfruit seeds as pharmaceutical excipients.

    PubMed

    Nayak, Amit Kumar; Pal, Dilipkumar; Santra, Kousik

    2015-08-01

    The paper describes the isolation and screening of plant polysaccharides namely tamarind seed polysaccharide (TSP), fenugreek seed mucilage (FSM) and jackfruit seed starch (JFSS) from tamarind (Tamarindus indica L.) seeds, fenugreek (Trigonella foenum-graecum L.) seeds and jackfruit (Artocarpus heterophyllus L.) seeds, respectively. The yields of isolated dried TSP, FSM and JFSS were 47.00%, 17.36% and 18.86%, respectively. Various physicochemical properties like colour, odour, taste, solubility in water, pH and viscosity of these isolated plant polysaccharides were assessed. Isolated polysaccharide samples were subjected to some phytochemical identification tests. FTIR and (1)H NMR analyses of isolated polysaccharides were performed, which suggest the presence of sugar residues. Isolated TSP, FSM and JFSS can be used as pharmaceutical excipients in various pharmaceutical formulations. Copyright © 2015 Elsevier B.V. All rights reserved.

  6. In vitro and cellular activities of the selected fruits residues for skin aging treatment.

    PubMed

    Lourith, Nattaya; Kanlayavattanakul, Mayuree; Chaikul, Puxvadee; Chansriniyom, Chaisak; Bunwatcharaphansakun, Pichaporn

    2017-05-01

    Peel extracts of litchi and rambutan, and that of tamarind seed coat were investigated in relation to their utility in skin-aging treatments. Standardized extracts of tamarind were significantly (p < 0.05) more efficient at O2 •- scavenging (IC50 = 27.44 ± 0.09) than those of litchi and rambutan (IC50 = 29.57 ± 0.30 and 39.49 ± 0.52 μg/ml, respectively) and the quercetin standard (IC50 = 31.88 ± 0.15 μg/ml). Litchi extract proved significantly (p < 0.05) more effective for elastase and collagenase inhibition (88.29 ± 0.25% and 79.46 ± 0.92%, respectively) than tamarind (35.43 ± 0.68% and 57.69 ± 5.97%) or rambutan (31.08 ± 0.38% and 53.99 ± 6.18%). All extracts were safe to human skin fibroblasts and inhibit MMP-2, with litchi extract showing significantly (p < 0.01) enhanced inhibition over the standard, vitamin C (23.75 ± 2.74% and 10.42 ± 5.91% at 0.05 mg/ml, respectively). Extracts suppress melanin production in B16F10 melanoma cells through inhibition of tyrosinase and TRP-2, with litchi extract being the most potent, even more so than kojic acid (standard). These results highlight the potential for adding value to agro-industrial waste, as the basis for the sustainable production of innovative, safe, anti-aging cosmetic products.

  7. Combustion of peanut and tamarind shells in a conical fluidized-bed combustor: a comparative study.

    PubMed

    Kuprianov, Vladimir I; Arromdee, Porametr

    2013-07-01

    Combustion of peanut and tamarind shells was studied in the conical fluidized-bed combustor using alumina sand as the bed material to prevent bed agglomeration. Morphological, thermogravimetric and kinetic characteristics were investigated to compare thermal and combustion reactivity between the biomass fuels. The thermogravimetric kinetics of the biomasses was fitted using the Coats-Redfern method. Experimental tests on the combustor were performed at 60 and 45 kg/h fuel feed rates, with excess air within 20-80%. Temperature and gas concentrations were measured along radial and axial directions in the reactor and at stack. The axial temperature and gas concentration profiles inside the combustor exhibited sensible effects of fuel properties and operating conditions on combustion and emission performance. High (≈ 99%) combustion efficiency and acceptable levels of CO, CxHy, and NO emissions are achievable when firing peanut shells at excess air of about 40%, whereas 60% is more preferable for burning tamarind shells. Copyright © 2013 Elsevier Ltd. All rights reserved.

  8. Cleansing lotion containing tamarind fruit pulp extract. II. Study of cumulative irritation effects in human.

    PubMed

    Maenthaisong, Ratree; Viyoch, Jarupa; Chaiyakunapruk, Nathorn; Warnnissorn, Prateep

    2007-09-01

    Cleansing lotion containing extract of tamarind fruit pulp was developed to provide skin a lighter effect. Skin irritation may occur due to keratolytic effect of alpha-hydroxyl acids (AHA) in the tamarind fruit pulp extract. To assess the cumulative irritation effect of cleansing lotion containing tamarind fruit extract with 2% (w/w) tartaric acid on human skin compared with placebo product and de-ionized water. The study design was a single-blinded, randomized side of arm, and controlled study. Three samples, including test product, placebo product, and de-ionized water, were repeatedly applied on the inner forearm of 15 healthy females (aged 28.3 +/- 3.1 years) for 30 min daily for 5 days under semi-occlusive patch. Skin irritation was measured by using visual scoring and instruments such as Tewameter and Mexameter. All measurements were done before application of samples every day from day 1 until day 5. Final measurements were done after the last application for 3 days (day 8). The results obtained from the visual scoring scale indicated no irritation signs and symptoms of test product. Mean differences of transepidermal water loss and erythema values between test product and de-ionized water and between test and placebo products were not statistically significant (P > 0.05). These findings indicate a preliminary safety evidence of our developed cleansing lotion containing the natural AHAs and can be used as cumulative evidence for supporting the future home use study of this product in human.

  9. Tannins of tamarind seed husk: preparation, structural characterization, and antioxidant activities.

    PubMed

    Sinchaiyakit, Puksiri; Ezure, Yohji; Sriprang, Sarin; Pongbangpho, Supakorn; Povichit, Nasapon; Suttajit, Maitree

    2011-06-01

    The high content (about 39%) of polymeric tannins in tamarind (Tamarindus indica L.) seed husk (TSH) was demonstrated, and an extract (crude TSE) with a high content (about 94%) of polymeric tannins was prepared from TSH with a one pot extraction using ethanol/water (3:2, v/v). The crude TSE was further purified with Sephadex LH20 to give one fraction (metTSE) eluted with methanol/water (3:2, v/v) and another (acTSE) eluted with acetone/water (3:2, v/v). The tannins of acTSE were established as polymeric proanthocyanidins (PA) by 13C NMR spectroscopy; this was further confirmed by IR and UV spectroscopy, n-BuOH/HCl and vanillin assays, and from HPLC pattern. The ratio of procyanidins to prodelphinidins was 2:3, and the average degree of polymerization of acTSE was 7. Galloylated flavan-3-ols were not detected in acTSE. The main ingredients of metTSE were confirmed to be polymeric PA by 13C NMR spectroscopy. The antioxidant activities using DPPH and ABTS assays were investigated. The IC50 values of acTSE were 4.2 +/- 0.2 (DPPH assay) and 6.2 +/- 0.3 microg/mL (ABTS assay).

  10. Gastroretentive behavior of orally administered radiolabeled tamarind seed formulations in rabbits validated by gamma scintigraphy.

    PubMed

    Razavi, Mahboubeh; Karimian, Hamed; Yeong, Chai Hong; Fadaeinasab, Mehran; Khaing, Si Lay; Chung, Lip Yong; Mohamad Haron, Didi Erwandi B; Noordin, Mohamed Ibrahim

    2017-01-01

    This study aimed to formulate floating gastroretentive tablets containing metformin hydrochloric acid (HCl), using various grades of hydrogel such as tamarind powders and xanthan to overcome short gastric residence time of the conventional dosage forms. Different concentrations of the hydrogels were tested to determine the formulation that could provide a sustained release of 12 h. Eleven formulations with different ratios of tamarind seed powder/tamarind kernel powder (TKP):xanthan were prepared. The physical parameters were observed, and in vitro drug-release studies of the prepared formulations were carried out. Optimal formulation was assessed for physicochemical properties, thermal stability, and chemical interaction followed by in vivo gamma scintigraphy study. MKP3 formulation with a TKP:xanthan ratio of 3:2 was found to have 99.87% release over 12 h. Furthermore, in vivo gamma scintigraphy study was carried out for the optimized formulation in healthy New Zealand White rabbits, and the pharmacokinetic parameters of developed formulations were obtained. 153 Sm 2 O 3 was used to trace the profile of release in the gastrointestinal tract of the rabbits, and the drug release was analyzed. The time ( T max ) at which the maximum concentration of metformin HCl in the blood ( C max ) was observed, and it was extended four times for the gastroretentive formulation in comparison with the formulation without polymers. C max and the half-life were found to be within an acceptable range. It is therefore concluded that MKP3 is the optimal formulation for sustained release of metformin HCl over a period of 12 h as a result of its floating properties in the gastric region.

  11. Gastroretentive behavior of orally administered radiolabeled tamarind seed formulations in rabbits validated by gamma scintigraphy

    PubMed Central

    Razavi, Mahboubeh; Karimian, Hamed; Yeong, Chai Hong; Fadaeinasab, Mehran; Khaing, Si Lay; Chung, Lip Yong; Mohamad Haron, Didi Erwandi B; Noordin, Mohamed Ibrahim

    2017-01-01

    This study aimed to formulate floating gastroretentive tablets containing metformin hydrochloric acid (HCl), using various grades of hydrogel such as tamarind powders and xanthan to overcome short gastric residence time of the conventional dosage forms. Different concentrations of the hydrogels were tested to determine the formulation that could provide a sustained release of 12 h. Eleven formulations with different ratios of tamarind seed powder/tamarind kernel powder (TKP):xanthan were prepared. The physical parameters were observed, and in vitro drug-release studies of the prepared formulations were carried out. Optimal formulation was assessed for physicochemical properties, thermal stability, and chemical interaction followed by in vivo gamma scintigraphy study. MKP3 formulation with a TKP:xanthan ratio of 3:2 was found to have 99.87% release over 12 h. Furthermore, in vivo gamma scintigraphy study was carried out for the optimized formulation in healthy New Zealand White rabbits, and the pharmacokinetic parameters of developed formulations were obtained. 153Sm2O3 was used to trace the profile of release in the gastrointestinal tract of the rabbits, and the drug release was analyzed. The time (Tmax) at which the maximum concentration of metformin HCl in the blood (Cmax) was observed, and it was extended four times for the gastroretentive formulation in comparison with the formulation without polymers. Cmax and the half-life were found to be within an acceptable range. It is therefore concluded that MKP3 is the optimal formulation for sustained release of metformin HCl over a period of 12 h as a result of its floating properties in the gastric region. PMID:28031701

  12. Effect of Additives on Green Sand Molding Properties using Design of Experiments and Taguchi's Quality Loss Function - An Experimental Study

    NASA Astrophysics Data System (ADS)

    Desai, Bhagyashree; Mokashi, Pavani; Anand, R. L.; Burli, S. B.; Khandal, S. V.

    2016-09-01

    The experimental study aims to underseek the effect of various additives on the green sand molding properties as a particular combination of additives could yield desired sand properties. The input parameters (factors) selected were water and powder (Fly ash, Coconut shell and Tamarind) in three levels. Experiments were planned using design of experiments (DOE). On the basis of plans, experiments were conducted to understand the behavior of sand mould properties such as compression strength, shear strength, permeability number with various additives. From the experimental results it could be concluded that the factors have significant effect on the sand properties as P-value found to be less than 0.05 for all the cases studied. The optimization based on quality loss function was also performed. The study revealed that the quality loss associated with the tamarind powder was lesser compared to other additives selected for the study. The optimization based on quality loss function and the parametric analysis using ANOVA suggested that the tamarind powder of 8 gm per Kg of molding sand and moisture content of 7% yield better properties to obtain sound castings.

  13. Screening of various botanical extracts for antioxidant activity using DPPH free radical method.

    PubMed

    Waqas, Muhammad Khurram; Saqib, Najam-Us; Rashid, Saeed-Ur; Shah, Pervaiz Akhtar; Akhtar, Naveed; Murtaza, Ghulam

    2013-01-01

    Aiming at the exploration of herbal use by society, crude extracts of the seeds of some commonly used medicinal plants (Vitis vinifera, Tamarindus indica and Glycin max) were screened for their free radical scavenging properties using ascorbic acid as standard antioxidant. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical. The overall antioxidant activity of grape seeds (Vitis vinifera) was the strongest, followed in descending order by soybean (Glycin max) and tamarind (Tamarindus indica). The seeds extract of Vitis vinifera, Glycin max and Tamarindus indica showed 85.61%, 83.45% and 79.26%, DPPH scavenging activity respectively.

  14. Structural and electrical characterization of tamarind seed polysaccharide (TSP) doped with NH4HCO2

    NASA Astrophysics Data System (ADS)

    Premalatha, M.; Mathavan, T.; Selvasekarapandian, S.; Selvalakshmi, S.

    2018-04-01

    In the modern era, development of electrochemical energy devices such as batteries, fuel cells and supercapacitors gain attention due to the deficiency of renewable energy resources. More specifically, proton conducting materials create prime interest in the development of electrochemical devices. In this regards, a novel proton conducting biopolymer electrolyte based on Tamarind Seed Polysaccharide (TSP) was synthesized with different concentration of ammonium formate (NH4HCO2). The amorphous nature of the polymer electrolytes has been identified by XRD technique. The observed ionic conductivity values reveal that the biopolymer containing 1 g TSP: 0.4 g NH4HCO2 has highest ionic conductivity 1.23×10-3 S cm-1.

  15. Formulation and development of a patch containing tamarind fruit extract by using the blended chitosan-starch as a rate-controlling matrix.

    PubMed

    Viyoch, J; Patcharaworakulchai, P; Songmek, R; Pimsan, V; Wittaya-Areekul, S

    2003-06-01

    A cosmetic patch containing tamarind fruit extract was formulated and developed by blending two types of natural polymers: chitosan with molecular weight of 100 000 and starch such as corn, potato or tapioca starch. The physicochemical characteristics, i.e. flexibility, colour, transparency, integrity, gloss, water sorption and bioadhesion property and the stability of the patch without tamarind content were investigated. Stability test was performed by keeping the prepared patches at 4 degrees C, at room temperature or at 45 degrees C for 2 weeks. The results showed that the formulations composed of chitosan:corn starch ratio of 4.5 : 0.5 (CC(4.5 : 0.5)) and chitosan:tapioca starch ratios of 4.5 : 0.5 (CT(4.5 : 0.5)) and 4.0 : 1.0 (CT(4 : 1)) provide patches with favourable physical characteristics, high water sorption, good bioadhesion ability and good stability. After the lyophilized tamarind extract in an amount corresponding to 5% of tartaric acid was incorporated into the formulations of CC(4.5 : 0.5), CT(4.5 : 0.5) and CT(4 : 1), the ability of the patches to adhere to skin was improved. However, after keeping the test patches at room temperature or at 45 degrees C for 6 weeks, their colours were intensified while their flexibilities and skin adhesion properties decreased. A 12-h in vitro permeation was investigated by studying the cumulative amount of tartaric acid permeated through the Silastic membrane (Dow-Coming, Midland, MI, USA). The CC(4.5 : 0.5) patch tended to give the highest amount of tartaric acid released. The release pattern of all the blended polymeric matrices was exhibited in two distinct phases: the rapid phase, where the flux averaged 3.61 microg min(-1) mm(-2); and the slow phase, where the flux averaged 1.89 microg min(-1) mm(-2).

  16. Effect of polyphenols extracted from tamarind ( Tamarindus indica L.) seed coat on pathophysiological changes and red blood cell glutathione peroxidase activity in heat-stressed broilers

    NASA Astrophysics Data System (ADS)

    Aengwanich, Worapol; Suttajit, Maitree

    2013-01-01

    The purpose of this study was to determine the effect of polyphenols extracted from the tamarind seed coat (PETSC) on glutathione peroxidase (GPx) activity, red blood cell parameters and bilirubin in heat-stressed broilers. One hundred forty-seven broilers, 18-days old were divided into two groups. In group 1, broilers were maintained at an environmental temperature of 26 ± 2 °C throughout the experimental period. In group 2, the broilers were maintained at 38 ± 2 °C (cyclic temperature: 26 ± 2 °C; -38 ± 2 °C; and -26 ± 2 °C, and broilers were maintained at 38 ± 2 °C for 6 h/ day) and received PETSC at a concentration of 0, 100, 200, 300, 400 or 500 mg/kg in their diet ad libitum. Parameters were investigated on days 1, 7, 14 and 21 of the experimental period. Results showed that GPx activity of heat-stressed broilers that received 100 mg/kg of PETSC in their diet was lower ( P < 0.05) than that in broilers fed the other concentrations. The mean total red blood cell count and hemoglobin concentration of heat-stressed broilers that received 100 mg/kg PETSC was higher ( P < 0.05) than those in broilers in group 1 and those fed the other concentrations. The mean bilirubin level in the excreta of heat-stressed broilers that received 100 mg/kg of PETSC was lower ( P < 0.05) than that in broilers that received 0, 300, 400 and 500 mg/kg of PETSC. This showed that PETSC could reduce GPx activity and bilirubin in feces, and increase red blood cell parameters in heat-stressed broilers.

  17. Mutagenic activity of south Indian food items.

    PubMed

    Sivaswamy, S N; Balachandran, B; Balanehru, S; Sivaramakrishnan, V M

    1991-08-01

    Dietary components and food dishes commonly consumed in South India were screened for their mutagenic activity. Kesari powder, calamus oil, palm drink, toddy and Kewra essence were found to be strongly mutagenic; garlic, palm oil, arrack, onion and pyrolysed portions of bread toast, chicory powder were weakly mutagenic, while tamarind and turmeric were not. Certain salted, sundried and oil fried food items were also mutagenic. Cissus quadrangularis was mutagenic, while 'decoctions' of cumin seeds, aniseeds and ginger were not. Several perfumes, essential oils and colouring agents, which are commonly used were also screened and many of them exhibited their mutagenic potential by inducing the 'reverse mutation' in Salmonella typhimurium tester strains.

  18. Gamma scintigraphic evaluation of floating gastroretentive tablets of metformin HCl using a combination of three natural polymers in rabbits

    PubMed Central

    Razavi, Mahboubeh; Karimian, Hamed; Yeong, Chai Hong; Chung, Lip Yong; Nyamathulla, Shaik; Noordin, Mohamed Ibrahim

    2015-01-01

    The present research was aimed at formulating a metformin HCl sustained-release formulation from a combination of polymers, using the wet granulation technique. A total of 16 formulations (F1–F16) were produced using different combinations of the gel-forming polymers: tamarind kernel powder, salep (palmate tubers of Orchis morio), and xanthan. Post-compression studies showed that there were no interactions between the active drug and the polymers. Results of in vitro drug-release studies indicated that the F10 formulation which contained 5 mg of tamarind kernel powder, 33.33 mg of xanthan, and 61.67 mg of salep could sustain a 95% release in 12 hours. The results also showed that F2 had a 55% similarity factor with the commercial formulation (C-ER), and the release kinetics were explained with zero order and Higuchi models. The in vivo study was performed in New Zealand White rabbits by gamma scintigraphy; the F10 formulation was radiolabeled using samarium (III) oxide (153Sm2O3) to trace transit of the tablets in the gastrointestinal tract. The in vivo data supported the retention of F10 formulation in the gastric region for 12 hours. In conclusion, the use of a combination of polymers in this study helped to develop an optimal gastroretentive drug-delivery system with improved bioavailability, swelling, and floating characteristics. PMID:26273196

  19. Formulation of mucoadhesive gastric retentive drug delivery using thiolated xyloglucan.

    PubMed

    Bhalekar, Mangesh R; Bargaje, Rajesh V; Upadhaya, Prashant G; Madgulkar, Ashwini R; Kshirsagar, Sanjay J

    2016-01-20

    Tamarind seed xyloglucan is a polymer reported to possess mucoadhesive property. In the present work, role of cysteine derivative of tamarind seed polysaccharide (thiomer) to enhance the mucoadhesion and its influence on drug permeation has been studied. The xyloglucan was first chemically modified to carboxymethyl derivative which was further converted to thiomer by conjugation with cysteine in presence of a coupling agent, EDAC. The matrix tablets of simvastatin prepared using thiomer demonstrated drug release retardation, increased mucoadhesion force and increased ex vivo permeation, the same were proportional to the increase in the amount of thiomer. The in vivo residence of thiomer placebo was more than 7h in rabbit. Pharmacokinetic evaluation in rabbits indicated higher AUC for the formulation with highest content of thiomer and level 'A' correlation could be established from the generated dissolution and bioavailability data. Copyright © 2015 Elsevier Ltd. All rights reserved.

  20. 7 CFR 1540.41 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... AGRICULTURE INTERNATIONAL AGRICULTURAL TRADE Emergency Relief From Duty-Free Imports of Perishable Products From Certain Andean Countries § 1540.41 Definitions. (a) Perishable product means: (1) Live plants and... subheadings 0805.90.00, tamarinds and kiwi fruit of subheading 0810.90.20, and cashew apples, mameyes...

  1. 7 CFR 1540.41 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AGRICULTURE INTERNATIONAL AGRICULTURAL TRADE Emergency Relief From Duty-Free Imports of Perishable Products From Certain Andean Countries § 1540.41 Definitions. (a) Perishable product means: (1) Live plants and... subheadings 0805.90.00, tamarinds and kiwi fruit of subheading 0810.90.20, and cashew apples, mameyes...

  2. 7 CFR 1540.41 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AGRICULTURE INTERNATIONAL AGRICULTURAL TRADE Emergency Relief From Duty-Free Imports of Perishable Products From Certain Andean Countries § 1540.41 Definitions. (a) Perishable product means: (1) Live plants and... subheadings 0805.90.00, tamarinds and kiwi fruit of subheading 0810.90.20, and cashew apples, mameyes...

  3. 7 CFR 1540.41 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... AGRICULTURE INTERNATIONAL AGRICULTURAL TRADE Emergency Relief From Duty-Free Imports of Perishable Products From Certain Andean Countries § 1540.41 Definitions. (a) Perishable product means: (1) Live plants and... subheadings 0805.90.00, tamarinds and kiwi fruit of subheading 0810.90.20, and cashew apples, mameyes...

  4. 7 CFR 1540.41 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... AGRICULTURE INTERNATIONAL AGRICULTURAL TRADE Emergency Relief From Duty-Free Imports of Perishable Products From Certain Andean Countries § 1540.41 Definitions. (a) Perishable product means: (1) Live plants and... subheadings 0805.90.00, tamarinds and kiwi fruit of subheading 0810.90.20, and cashew apples, mameyes...

  5. 40 CFR 180.507 - Azoxystrobin; tolerances for residues.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Sapote, mamey 2.0 Sapote, white 2.0 Sorghum, grain, forage 25 Sorghum, grain, grain 11 Sorghum, grain... Sugarcane, cane 0.2 Sunflower subgroup 20B 0.5 Tamarind 2.0 Tomato, paste 0.6 Tomato subgroup 8-10A 0.2...

  6. 40 CFR 180.507 - Azoxystrobin; tolerances for residues.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Sapote, mamey 2.0 Sapote, white 2.0 Sorghum, grain, forage 25 Sorghum, grain, grain 11 Sorghum, grain... Sugarcane, cane 0.2 Sunflower subgroup 20B 0.5 Tamarind 2.0 Tomato, paste 0.6 Tomato subgroup 8-10A 0.2...

  7. Effect of feeding tamarind kernel powder extract residue on digestibility, nitrogen availability and ruminal fermentation in wethers.

    PubMed

    Wang, Lin; Nakanishi, Takashi; Sato, Yoshiaki; Oishi, Kazato; Hirooka, Hiroyuki; Takahashi, Kei; Kumagai, Hajime

    2017-03-01

    This study was to examine in vivo digestibility, nitrogen balance and ruminal fermentation of tamarind ( Tamarind indica ) kernel powder extract residue (TKPER) compared to soybean products and by-products in wethers. Four wethers with initial body weight (BW) of 51.6±5.5 kg were assigned in a 4×4 Latin square design to investigate nutritional characteristics of TKPER, dry heat soybean (SB), dry soybean curd residue (SBCR) and soybean meal (SBM) feeding with ryegrass straw (R) at a ratio of 1:1 at 2% of BW in dry matter (DM) on a daily basis. The digestibility of DM, crude protein, and ether extract (EE) of TKPER-R diet were 57.0%, 87.0%, and 86.0%, respectively. Higher non-fiber carbohydrates digestibility was observed in TKPER-R diet (83.2%) than in SB-R diet (73.9%, p<0.05). Wethers fed the TKPER-R diet had lower retention of nitrogen (N) and ruminal ammonia nitrogen (NH 3 -N) contents at 4 h after feeding than those fed the SBM-R diet (p<0.05), which had values similar to the SB-R or SBCR-R diet. The TKPER feeding had higher propionate (C3) and lower butyrate content, as well as lower acetate to propionate ratio (C2:C3) in rumen fluid than SBM feeding at 4 h after feeding (p<0.05). TKPER did not bring any side effect to the wethers although it was lack of fiber, and could be used as a high protein and energy ingredient in concentrate with appropriate roughage to meet the fiber requirement for ruminants.

  8. Bioactivities in the tamarind seed extracts: A preliminary study

    NASA Astrophysics Data System (ADS)

    Garg, Sukant; Muangman, Thanchanok; Huifu, He; Ling, Li; Kaul, Sunil C.; Wadhwa, Renu

    2018-01-01

    Stress is a state that triggers change in normal physiology and recognized by human body and brain as an unfavorable event causing concern, worry or anxiety. It may vary from physical, metabolic, physiological or emotional often culminating into wide range of ailments that may range from common cold, decline in functional efficacy of body systems or even cancer. Skin is the largest tissue of the body and makes the first interface with the environment. Skin color and characteristics are highly influenced by environment stress. A variety of natural compounds have been used for anti-stress and disease preventive potentials in worldwide traditional home medicine systems. They have recently attracted attention in research laboratories to dissect their mode of action to promote safe and economic drug development. We have earlier identified anti-stress and anti-aging activities in Withania somnifera, Helicteres angustifolia and honeybee propolis using human cultured normal and cancer cells. In the present study, we explored the effect of tamarind seed extracts prepared in water or 95% ethanol. In cell-based assays, we found that the extracts were safe to use in viable cells (in the range of 0.01-1.0%, for at least 4 weeks). Consistently, molecular studies revealed no effect on the expression/activity of cancer promoting proteins. We recruited oxidative stress models, such as, hydrogen peroxide (H2O2), ultraviolet radiation (UV) and diacylglycerol 1-oleoyl-2-acetyl-sn-glycerol (OAG). Investigation on anti-stress potential of the extracts revealed that they do not offer remarkable protection against stress caused by either H2O2 or UV, however, significantly compromised OAG-induced melanogenesis. The preliminary data warrant further investigations on the active components and mechanism of action to develop useful natural compounds/extracts for manipulation of melanogenesis that plays important role in response of cells to UV and its consequences including DNA damage, oxidative stress and related diseases.

  9. Property evaluations of dry-cast reconstituted bacterial cellulose/tamarind xyloglucan biocomposites.

    PubMed

    de Souza, Clayton F; Lucyszyn, Neoli; Woehl, Marco A; Riegel-Vidotti, Izabel C; Borsali, Redouane; Sierakowski, Maria Rita

    2013-03-01

    We describe the mechanical defibrillation of bacterial cellulose (BC) followed by the dry-cast generation of reconstituted BC films (RBC). Xyloglucan (XGT), extracted from tamarind seeds, was incorporated into the defibrillated cellulose at various compositions, and new films were created using the same process. Microscopy and contact angle analyses of films revealed an increase in the microfibre adhesion, a reduced polydispersity in the diameters of the microfibrils and increased hydrophobic behaviour as a function of %XGT. X-ray diffraction analysis revealed changes to the crystallographic planes of the RBC and the biocomposite films with preferential orientation along the (110) plane. Compared with BC, RBC/XGT biocomposite with 10% XGT exhibited improvement in its thermal properties and in Young's modulus. These results indicated a reorganisation of the microfibres with mechanical treatment, which when combined with hydrocolloids, can create cellulose-based materials that could be applied as scaffolding for tissue engineering and drug release. Copyright © 2012 Elsevier Ltd. All rights reserved.

  10. Control of oenological products: discrimination between different botanical sources of L-tartaric acid by isotope ratio mass spectrometry.

    PubMed

    Moreno Rojas, Jose Manuel; Cosofret, Sorin; Reniero, Fabiano; Guillou, Claude; Serra, Francesca

    2007-01-01

    Following previous studies on counterfeit of wines with synthetic ingredients, the possibility of frauds by natural external L-tartaric acid has also been investigated. The aim of this research was to map the stable isotope ratios of L-tartaric acid coming from botanical species containing large amounts of this compound: grape and tamarind. Samples of L-tartaric acid were extracted from the pulp of tamarind fruits originating from several countries and from grape must. delta(13)C and delta(18)O were measured for all samples. Additional delta(2)H measurements were performed as a complementary analysis to help discrimination of the botanical origin. Different isotopic patterns were observed for the different botanical origins. The multivariate statistical analysis of the data shows clear discrimination among the different botanical and synthetic sources. This approach could be a complementary tool for the control of L-tartaric acid used in oenology. Copyright (c) 2007 John Wiley & Sons, Ltd.

  11. Antimicrobial activity of extracts from Tamarindus indica L. leaves

    PubMed Central

    Escalona-Arranz, Julio César; Péres-Roses, Renato; Urdaneta-Laffita, Imilci; Camacho-Pozo, Miladis Isabel; Rodríguez-Amado, Jesús; Licea-Jiménez, Irina

    2010-01-01

    Tamarindus indica L. leaves are reported worldwide as antibacterial and antifungal agents; however, this observation is not completely accurate in the case of Cuba. In this article, decoctions from fresh and sun dried leaves, as well as fluid extracts prepared with 30 and 70% ethanol-water and the pure essential oil from tamarind leaves were microbiologically tested against Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, Pseudomona aeruginosa and Candida albicans. Aqueous and fluid extracts were previously characterized by spectrophotometric determination of their total phenols and flavonoids, while the essential oil was chemically evaluated by gas chromatography/mass spectroscopy (GC/MS). Experimental data suggest phenols as active compounds against B. subtilis cultures, but not against other microorganisms. On the other hand, the essential oil exhibited a good antimicrobial spectrum when pure, but its relative low concentrations in common folk preparations do not allow for any good activity in these extracts. PMID:20931087

  12. Tannase production by Penicillium purpurogenum PAF6 in solid state fermentation of tannin-rich plant residues following OVAT and RSM.

    PubMed

    Jana, Arijit; Maity, Chiranjit; Halder, Suman Kumar; Mondal, Keshab Chandra; Pati, Bikash Ranjan; Mohapatra, Pradeep Kumar Das

    2012-07-01

    Tannase production by newly isolated Penicillium purpurogenum PAF6 was investigated by 'one variable at a time' (OVAT) approach followed by response surface methodology (RSM). Tannin-rich plant residues were used as supporting solid substrate and sole carbon source and, among them, tamarind seed was found to be the most favorable substrate than haritaki, pomegranate, tea leaf waste and arjun fruit. Physicochemical parameters were initially optimized using OVAT methodology and some important factors like incubation time, incubation temperature, substrate:moisture ratio as well as carbon, nitrogen and phosphate concentrations were verified with Box-Behken design of response surface methodology. Phosphate source, nitrogen source and temperature were found as the most favorable variables in the maximization of production. Tannase production was enhanced from 1.536 U/g to 5.784 U/g using tamarind seed OVAT optimization and further enhancement up to 6.15 U/g following RSM. An overall 3.76- and 4.0-fold increases in tannase production were achieved in OVAT and RSM, respectively.

  13. Acceptability and solubility of iron and zinc contents of modified Moringa oleifera sauces consumed in the Far-north region of Cameroon.

    PubMed

    Mawouma, Saliou; Ponka, Roger; Mbofung, Carl Moses

    2017-03-01

    Consumption of Moringa oleifera leaves is a local and inexpensive solution to iron and zinc deficiencies in the Far-north region of Cameroon. However, traditional household's cooking techniques result in sauces with high pH levels and low leaves incorporation rates that compromise the bioavailability of iron and zinc. The aim of our study was to investigate the effect of modifying a standard Moringa sauce on consumer acceptability and the solubility of iron and zinc, which is an indicator of their bioavailability. Lime juice or tamarind pulp was added to a standard recipe in order to reduce the pH by about one unit, and Moringa leaf powder was incorporated in each acidulated sauce at three levels (1, 2, and 4 g/100 g of sauce). All the formulations were evaluated for their acceptability by 30 housewives using a five-point hedonic scale. The pH was measured by a digital electronic pH-meter. Moisture and ash were determined by AOAC methods. Total iron and zinc contents were determined by atomic absorption spectrophotometry, and soluble iron and zinc by HCl-extractability. The lime juice-acidulated sauce and the tamarind pulp-acidulated sauce enriched with 1 g of Moringa leaf powder were the most acceptable formulations with scores of 3.4 and 3.6, respectively. Their chemical analysis showed a reduced pH (6.4 and 6.1, respectively), compared to the Control (7.2). Lime juice-acidulated sauce improved iron and zinc solubility from 42.19 to 66.38% and 54.03 to 82.03%, respectively. Tamarind pulp-acidulated sauce enriched with 1 g of Moringa leaf powder showed a decrease in iron solubility from 42.19 to 38.26% and an increase in zinc solubility from 54.03 to 72.86%. These results confirm the beneficial effect of lime juice in improving iron and zinc bioavailability.

  14. Pectic homogalacturonan masks abundant sets of xyloglucan epitopes in plant cell walls.

    PubMed

    Marcus, Susan E; Verhertbruggen, Yves; Hervé, Cécile; Ordaz-Ortiz, José J; Farkas, Vladimir; Pedersen, Henriette L; Willats, William G T; Knox, J Paul

    2008-05-22

    Molecular probes are required to detect cell wall polymers in-situ to aid understanding of their cell biology and several studies have shown that cell wall epitopes have restricted occurrences across sections of plant organs indicating that cell wall structure is highly developmentally regulated. Xyloglucan is the major hemicellulose or cross-linking glycan of the primary cell walls of dicotyledons although little is known of its occurrence or functions in relation to cell development and cell wall microstructure. Using a neoglycoprotein approach, in which a XXXG heptasaccharide of tamarind seed xyloglucan was coupled to BSA to produce an immunogen, we have generated a rat monoclonal antibody (designated LM15) to the XXXG structural motif of xyloglucans. The specificity of LM15 has been confirmed by the analysis of LM15 binding using glycan microarrays and oligosaccharide hapten inhibition of binding studies. The use of LM15 for the analysis of xyloglucan in the cell walls of tamarind and nasturtium seeds, in which xyloglucan occurs as a storage polysaccharide, indicated that the LM15 xyloglucan epitope occurs throughout the thickened cell walls of the tamarind seed and in the outer regions, adjacent to middle lamellae, of the thickened cell walls of the nasturtium seed. Immunofluorescence analysis of LM15 binding to sections of tobacco and pea stem internodes indicated that the xyloglucan epitope was restricted to a few cell types in these organs. Enzymatic removal of pectic homogalacturonan from equivalent sections resulted in the abundant detection of distinct patterns of the LM15 xyloglucan epitope across these organs and a diversity of occurrences in relation to the cell wall microstructure of a range of cell types. These observations support ideas that xyloglucan is associated with pectin in plant cell walls. They also indicate that documented patterns of cell wall epitopes in relation to cell development and cell differentiation may need to be re-considered in relation to the potential masking of cell wall epitopes by other cell wall components.

  15. Corticosterone level and central dopaminergic activity involved in agile and exploratory behaviours in formosan wood mice (Apodemus semotus).

    PubMed

    Shieh, Kun-Ruey; Yang, Shu-Chuan

    2018-03-27

    The native Formosan wood mouse (Apodemus semotus) is the dominant rodent in Taiwan. In their natural environment, Formosan wood mice exhibit high locomotor activity, including searching and exploratory behaviours, which is observed similarly in the laboratory environment. How the behavioural responses of Formosan wood mice exhibit in elevated plus maze and marble burying tests remains unclear. How corticosterone levels and central dopaminergic activities are related to the behaviours in these tests is also unclear. This study compared the behaviours of Formosan wood mice with that of C57BL/6J mice using the elevated plus maze and marble burying tests, and measured the corticosterone levels and central dopaminergic activities. Formosan wood mice showed greater locomotor and exploratory activity than the C57BL/6J mice. Similarly, the marble burying and rearing numbers were higher for Formosan wood mice. High locomotor and exploratory behaviours were strongly correlated with corticosterone levels after acute mild restraint stress in Formosan wood mice. The anxiolytic, diazepam, reduced the high exploratory activity, corticosterone levels and central dopaminergic activities. The high locomotor and exploratory behaviours of Formosan wood mice are related to the corticosterone levels and central dopaminergic activities. These data may explain Formosan wood mice dominance in the intermediate altitude of Taiwan.

  16. Southern Woods-Burners: A Descriptive Analysis

    Treesearch

    M.L. Doolittle; M.L. Lightsey

    1979-01-01

    About 40 percent of the South's nearly 60,000 wildfires yearly are set by woods-burners. A survey of 14 problem areas in four southern States found three distinct sets of woods-burners. Most active woods-burners are young, white males whose activities are supported by their peers. An older but less active group have probably retired from active participation but...

  17. Classroom Demonstrations of Wood Properties.

    ERIC Educational Resources Information Center

    Foulger, A. N.

    Presented in this manual are 20 activities selected to show some of the properties of wood and how these properties relate to the cellular structure of wood. Each activity includes stated objectives, indicates materials needed, and explains procedures. Illustrations related to the activities, glossary of terms, and photographs of wood structure…

  18. Tamarind seed gum-hydrolyzed polymethacrylamide-g-gellan beads for extended release of diclofenac sodium using 32 full factorial design.

    PubMed

    Nandi, Gouranga; Nandi, Amit Kumar; Khan, Najim Sarif; Pal, Souvik; Dey, Sibasish

    2018-07-15

    Development of tamarind seed gum (TSG)-hydrolyzed polymethacrylamide-g-gellan (h-Pmaa-g-GG) composite beads for extended release of diclofenac sodium using 3 2 full factorial design is the main purpose of this study. The ratio of h-Pmaa-g-GG and TSG and concentration of cross-linker CaCl 2 were taken as independent factors with three different levels of each. Effects of polymer ratio and CaCl 2 on drug entrapment efficiency (DEE), drug release, bead size and swelling were investigated. Responses such as DEE and different drug release parameters were statistically analyzed by 3 2 full factorial design using Design-Expert software and finally the formulation factors were optimized to obtain USP-reference release profile. Drug release rate was found to decrease with decrease in the ratio of h-Pmaa-g-GG:TSG and increase in the concentration of Ca 2+ ions in cross-linking medium. The optimized formulation showed DEE of 93.25% and an extended drug release profile over a period of 10h with f 2 =80.13. Kinetic modeling unveiled case-I-Fickian diffusion based drug release mechanism. Copyright © 2018 Elsevier B.V. All rights reserved.

  19. Synthesis, characterization and applications of a new cation exchanger tamarind sulphonic acid (TSA) resin.

    PubMed

    Singh, A V; Sharma, Naresh Kumar; Rathore, Abhay S

    2012-01-01

    A new composite cation exchanger, tamarind sulphonic acid (TSA) resin has been synthesized. The chemically modified TSA ion exchange resin has been used for the removal and preconcentration of Zn2+, Cd2+, Fe2+, Co2+ and Cu2+ ions in aqueous solution and effluent from the Laxmi steel plant in Jodhpur, India. This type of composite represents a new class of hybrid ion exchangers with good ion exchange capacity, stability, reproducibility and selectivity for toxic metal ions found in effluent from the steel industry. The characterization of the resin was carried out by determining the ion-exchange capacity, elemental analysis, pH titration, Fourier transform infrared spectra and thermal analysis. The distribution coefficients (K(d)) of toxic metal ions were determined in a reference aqueous solution and the steel plant effluent at different pH values; the absorbency of different metal ions on the TSA resin was studied for up to 10 cycles. The adsorption of different metal ions on TSA resin follows the order: Co2+ > Cu2+ > Zn2+ > Fe2+ > Cd2+. The ion exchange capacity of TSA resin is 2.87%.

  20. Activities of some enzymes of lignin formation in reaction wood of Thuja orientalis, Metasequoia glyptostroboides and Robinia pseudoacacia.

    PubMed

    Kutsuki, H; Higuchi, T

    1981-07-01

    The activities of the following five enzymes which are involved in the formation of lignin have been compared in reaction wood and in opposite wood: phenylalanine ammonia lyase (EC 4.3.1.5), caffeate 3-O-methyltransferase (EC 2.1.1.-), p-hydroxycinnamate: CoA ligase (EC 6.2.1.12), cinnamyl alcohol dehydrogenase (EC 1.1.1.-) and peroxidase (EC 1.11.1.7). The activities of the four first-named enzymes in the compression wood of Thuja orientalis L. and Metasequoia glyptostroboides Hu et Cheng were 2.8±1.4-fold and 2.6±1.5-fold higher than those in opposite wood, respectively, whereas peroxidase had the same level of activity in either type of wood. On the other hand, no differences were observed in the activities of the five enzymes between tension and opposite woods of Robinia pseudoacacia L. These findings are well in accord with the chemical structure of lignin in the compression and tension woods of the three species studied: high content of lignin rich in condensed units in compression wood, and little difference in lignin between tension and opposite woods.

  1. Root strength of tropical plants - An investigation in the Western Ghats of Kerala, India

    NASA Astrophysics Data System (ADS)

    Lukose Kuriakose, S.; van Beek, L. P. H.; van Westen, C. J.

    2009-04-01

    Earlier research on debris flows in the Tikovil River basin of the Western Ghats concluded that root cohesion is significant in maintaining the overall stability of the region. In this paper we present the most recent results (December 2008) of root tensile strength tests conducted on nine species of plants that are commonly found in the region. They are 1) Rubber (Hevea Brasiliensis), 2) Coconut Palm (Cocos nucifera), 3) Jackfruit trees (Artocarpus heterophyllus), 4) Teak (Tectona grandis), 5) Mango trees (Mangifera indica), 6) Lemon grass (Cymbopogon citratus), 7) A variety of Tamarind (Garcinia gummigutta), 8) Coffee (Coffea Arabica) and Tea (Camellia sinensis). About 1500 samples were collected of which only 380 could be tested (in the laboratory) due to breakage of roots during the tests. In the successful tests roots failed in tension. Roots having diameters between 2 mm and 12 mm were tested. Each sample tested has a length of 15 cm. Results indicate that the roots of Coffee, Tamarind, Lemon grass and Jackfruit are the strongest of the nine plant types tested whereas Tea and Teak plants had the most fragile roots. Coconut roots behaved atypical to the others, as the bark of the roots was crushed and slipped from the clamp when tested whereas its internal fiber was the strongest of all tested. Root tensile strength decreases with increasing diameters, Rubber showing more ductile behaviour than Coffee and Tamarind that behaved more brittle, root tensile strength increasing exponentially for finer roots. Teak and Tea showed almost a constant root tensile strength over the range of diameters tested and little variability. Jack fruit and mango trees showed the largest variability, which may be explained by the presence of root nodules, preventing the derivation of an unequivocal relationship between root diameters and tensile strength. This results in uncertainty of root strength estimates that are applicable. These results provide important information to quantify the upper limit of the root cohesion at the stand level in combination with land use maps. This is an indispensable component in the evaluation of slope stability in the region.

  2. Ecology of coarse wood decomposition by the saprotrophic fungus Fomes fomentarius.

    PubMed

    Větrovský, Tomáš; Voříšková, Jana; Snajdr, Jaroslav; Gabriel, Jiří; Baldrian, Petr

    2011-07-01

    Saprotrophic wood-inhabiting basidiomycetes are the most important decomposers of lignin and cellulose in dead wood and as such they attracted considerable attention. The aims of this work were to quantify the activity and spatial distribution of extracellular enzymes in coarse wood colonised by the white-rot basidiomycete Fomes fomentarius and in adjacent fruitbodies of the fungus and to analyse the diversity of the fungal and bacterial community in a fungus-colonised wood and its potential effect on enzyme production by F. fomentarius. Fungus-colonised wood and fruitbodies were collected in low management intensity forests in the Czech Republic. There were significant differences in enzyme production by F. fomentarius between Betula pendula and Fagus sylvatica wood, the activity of cellulose and xylan-degrading enzymes was significantly higher in beech wood than in birch wood. Spatial analysis of a sample B. pendula log segment proved that F. fomentarius was the single fungal representative found in the log. There was a high level of spatial variability in the amount of fungal biomass detected, but no effects on enzyme activities were observed. Samples from the fruiting body showed high β-glucosidase and chitinase activities compared to wood samples. Significantly higher levels of xylanase and cellobiohydrolase were found in samples located near the fruitbody (proximal), and higher laccase and Mn-peroxidase activities were found in the distal ones. The microbial community in wood was dominated by the fungus (fungal to bacterial DNA ratio of 62-111). Bacterial abundance composition was lower in proximal than distal parts of wood by a factor of 24. These results show a significant level of spatial heterogeneity in coarse wood. One of the explanations may be the successive colonization of wood by the fungus: due to differential enzyme production, the rates of biodegradation of coarse wood are also spatially inhomogeneous.

  3. Lectins in fruits having gastrointestinal activity: their participation in the hemagglutinating property of Escherichia coli O157:H7.

    PubMed

    Coutiño-Rodríguez, R; Hernández-Cruz, P; Giles-Ríos, H

    2001-01-01

    In fruits with therapeutic properties for antidiarrheal and laxative uses, the presence of lectins may be the bioactive properties that interfere with bacterial adhesion, thought to be competition for glycoside signal sites in the attachment. This study identifies lectins in crude extracts from fruits such as Tamarindus indica (tamarind), Spontia vulgaris (plum), Psidium guava (guava), Mangifera indica (mango), Cydonia vulgaris (quince), and Crataegus mexicanus (tejocote). To verify the procedures, extracts from Ricinus communis (castor bean), Glycine max (soybean), Phaseolus vulgaris (beans), Vicia fava (fava bean), and Solanum tuberosum (potato) were used as controls for lectin activity. Both sources of lectins were analyzed to determine their participation in the host-parasite interaction, using as a model the hemagglutinating properties of Escherichia coli O157:H7 (EHA). All extracts showed hemagglutination to group O erythrocytes test (HA) with the exception of mango. Two new galactose-specific lectins were identified from tamarind and guava. When analyzed for participation in EHA, only guava lectins inhibited this, while soybean lectin induced hemolysis; as both lectins bind to galactose, it is probable that their recognition occurs in different domains. Sugars involved in the attachment between Escherichia coli O157:H7 and red cells were identified and again, galactose in addition to mannose was found to be related in EHA. On the other hand, guava lectins also agglutinated E. coli O157:H7, perhaps due to the same galactose-specific lectin or to another type of lectin. In summary, guava has a galactose-specific lectin that prevents adhesion of E. coli O157:H7 to red cells; this lectin is mediated by galactose. Prevention could also be due to their capacity of agglutinating E. coli by guava lectins. Soybean lectin induced hemolysis only when bacteria was present, but not with floating secretions. This finding showed that guava is a source of lectin that can be explored to prevent adhesion of E. coli to epithelial intestinal cells; contrariwise, soya must be studied to see its participation in the uremia caused during E. coli O157:H7 pathogenesis.

  4. Antibacterial and antifungal activities of Euroschinus papuanus.

    PubMed

    Khan, M R; Omoloso, A D; Kihara, M

    2004-06-01

    The crude methanolic extracts of the leaves, stem bark, stem heart wood, root bark and root heart wood of Euroschinus papuanus and the fractions obtained on partitioning with petrol, dichloromethane (D), ethyl acetate (E) and butanol (B), exhibited a broad spectrum antibacterial activity. Fractionation drastically enhanced the activity. Excellent activity was demonstrated by the E fractions of stem heart wood, D of root bark, and E of root heart wood. Antifungal activity was exhibited by the B fractions of leaves, stem heartwood and root bark. Copyright 2004 Elsevier B.V.

  5. Past Cultural Restrictions in Anita Rau Badami's "Can You Hear the Night Bird Call?" and "'Tamarind Mem"

    ERIC Educational Resources Information Center

    Johny, S.

    2014-01-01

    The objective of the thesis is to bring out the trauma of the immigrants who are stuck up by the nostalgic and glorious past in their alien world. The cultural and social restrictions faced by the characters who live in their separate but intertwined worlds are brought in a detailed manner. Anita Rau Badami, one of the newest writers in the field…

  6. Synthesis and characterization of hydrogel films of carboxymethyl tamarind gum using citric acid.

    PubMed

    Mali, Kailas K; Dhawale, Shashikant C; Dias, Remeth J

    2017-12-01

    The objective of this study was to synthesize and characterize citric acid crosslinked carboxymethyl tamarind gum (CMTG) hydrogels films. The hydrogel films were characterized by Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, solid state 13 C-nuclear magnetic resonance ( 13 C NMR) spectroscopy and differential scanning calorimeter (DSC). The prepared hydrogel films were evaluated for the carboxyl content and swelling ratio. The model drug moxifloxacin hydrochloride was loaded into hydrogels films and drug release was studied at pH 7.4. The hemolysis assay was used to study the biocompatibility of hydrogel films. The results of ATR-FTIR, solid state 13 C NMR and DSC confirmed the formation of ester crosslinks between citric acid and CMTG. The total carboxyl content of hydrogel film was found to be decreased when amount of CMTG was increased. The swelling of hydrogel film was found to be decreased with increase in curing temperature and time. CMTG hydrogel films showed high drug loading with non-Fickian release mechanism suggesting controlled release of drug. The hydrogel films were found to be biocompatible. It can be concluded that the citric acid can be used for the preparation of CMTG hydrogel films. Further, CMTG hydrogel film can be used potentially for controlled release of drug. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. Reinforcing the inner phase of the filled hydrogels with CNTs alters drug release properties and human keratinocyte morphology: A study on the gelatin- tamarind gum filled hydrogels.

    PubMed

    Maharana, Vivek; Gaur, Deepanjali; Nayak, Suraj K; Singh, Vinay K; Chakraborty, Subhabrata; Banerjee, Indranil; Ray, Sirsendu S; Anis, Arfat; Pal, Kunal

    2017-11-01

    The study reports the synthesis and characterization of gelatin-tamarind gum (TG) based filled hydrogels for drug delivery applications. In this study, three different types of carbon nanotubes (CNTs) were incorporated within the dispersed TG phase of the filled hydrogels. The prepared hydrogels were thoroughly characterised using bright field microscope, FESEM, FTIR spectroscopy, differential scanning calorimeter, and mechanical tester. The swelling and the drug (salicylic acid) release properties of the filled hydrogels were also evaluated. The micrographs revealed the formation of biphasic systems. The internal phase appeared as agglomerates, and the CNTs were confined within the dispersed TG phase. FTIR and XRD studies revealed that CNTs promoted associative interactions among the components of the hydrogel, which promoted the formation of large crystallite size. The mechanical study indicated better resistance to the breakdown of the architecture of the CNT-containing filled hydrogels. Drug release studies, both passive and iontophoretic, suggested that the non-Fickian diffusion of the drug was prevalent during its release from hydrogel matrices. The prepared hydrogels were cytocompatible with human keratinocytes. The results suggested the probable use of such hydrogels in wound healing, tissue engineering and drug delivery applications. Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. Synthesis and characterization of PEPO grafted carboxymethyl guar and carboxymethyl tamarind as new thermo-associating polymers.

    PubMed

    Gupta, Nivika R; Torris A T, Arun; Wadgaonkar, Prakash P; Rajamohanan, P R; Ducouret, Guylaine; Hourdet, Dominique; Creton, Costantino; Badiger, Manohar V

    2015-03-06

    New thermo associating polymers were designed and synthesized by grafting amino terminated poly(ethylene oxide-co-propylene oxide) (PEPO) onto carboxymethyl guar (CMG) and carboxymethyl tamarind (CMT). The grafting was performed by coupling reaction between NH2 groups of PEPO and COOH groups of CMG and CMT using water-soluble EDC/NHS as coupling agents. The grafting efficiency and the temperature of thermo-association, T(assoc) in the copolymer were studied by NMR spectroscopy. The graft copolymers, CMG-g-PEPO and CMT-g-PEPO exhibited interesting thermo-associating behavior which was evidenced by the detailed rheological and fluorescence measurements. The visco-elastic properties (storage modulus, G'; loss modulus, G") of the copolymer solutions were investigated using oscillatory shear experiments. The influence of salt and surfactant on the T(assoc) was also studied by rheology, where the phenomenon of "Salting out" and "Salting in" was observed for salt and surfactant, respectively, which can give an easy access to tunable properties of these copolymers. These thermo-associating polymers with biodegradable nature of CMG and CMT can have potential applications as smart injectables in controlled release technology and as thickeners in cosmetics and pharmaceutical formulations. Copyright © 2014 Elsevier Ltd. All rights reserved.

  9. New views on antidiarrheal effect of wood creosote: is wood creosote really a gastrointestinal antiseptic?

    PubMed

    Ataka, Koji; Ito, Masafumi; Shibata, Takashi

    2005-12-01

    Wood creosote, the principal ingredient in Seirogan, has a long history as a known gastrointestinal microbicidal agent. When administered orally, the intraluminal concentration of wood creosote is not sufficiently high to achieve this microbicidal effect. Through further animal tests, we have shown that antimotility and antisecretory actions are the principal antidiarrheal effects of wood creosote. Wood creosote inhibits intestinal secretion induced by enterotoxins by blocking the Cl(-) channel on the intestinal epithelium. Wood creosote also decreases intestinal motility accelerated by mechanical, chemical, or electrical stimulus by the inhibition of the Ca(2+) influx into the smooth muscle cells. In this overview, the antimotility and antisecretory effects of wood creosote are compared with those of loperamide. Wood creosote was observed to inhibit stimulated colonic motility, but not normal jejunal motility. Loperamide inhibits normal jejunal motility, but not stimulated colonic motility. Both wood creosote and loperamide inhibit intestinal secretion accelerated by acetylcholine. Wood creosote was found to have greater antisecretory effects in the colon than loperamide. Based upon these findings, we conclude that the antidiarrheal effects of wood creosote are due to both antisecretory activity in the intestine and antimotility in the colon, but not due to the microbicidal activity as previously thought. Wood creosote was found to have no effects on normal intestinal activity. These conclusions are supported by the results of a recent clinical study comparing wood creosote and loperamide, which concluded that wood creosote was more efficacious in relieving abdominal pain and comparable to loperamide in relieving diarrhea.

  10. Antioxidant and toxicological evaluation of a Tamarindus indica L. leaf fluid extract.

    PubMed

    Escalona-Arranz, J C; Perez-Rosés, R; Rodríguez-Amado, J; Morris-Quevedo, H J; Mwasi, L B; Cabrera-Sotomayor, O; Machado-García, R; Fong-Lórez, O; Alfonso-Castillo, A; Puente-Zapata, E

    2016-01-01

    In the scientific community, there is a growing interest in Tamarindus indica L. leaves, both as a valuable nutrient and as a functional food. This paper focuses on exploring its safety and antioxidant properties. A tamarind leaf fluid extract (TFE) wholly characterised was evaluated for its anti-DPPH activity (IC50 = 44.36 μg/mL) and its reducing power activity (IC50 = 60.87 μg/mL). TFE also exhibited a high ferrous ion-chelating capacity, with an estimated binding constant of 1.085 mol L(-1) while its influence over nitric oxide production in human leucocytes was irregular. At low concentrations, TFE stimulated NO output, but it significantly inhibited it when there was an increase in concentration. TFE was also classified as a non-toxic substance in two toxicity tests: the acute oral toxicity test and the oral mucous irritability test. Further toxicological assays are needed, although results so far suggest that TFE might become a functional dietary supplement.

  11. Chemical Analysis and Study of Phenolics, Antioxidant Activity, and Antibacterial Effect of the Wood and Bark of Maclura tinctoria (L.) D. Don ex Steud.

    PubMed Central

    Lamounier, K. C.; Cunha, L. C. S.; de Morais, S. A. L.; de Aquino, F. J. T.; Chang, R.; do Nascimento, E. A.; de Souza, M. G. M.; Martins, C. H. G.; Cunha, W. R.

    2012-01-01

    Maclura tinctoria (L.) D. Don ex Steud. has one of the highest qualities among the coefficients for Brazilian woods (up to 9.6) and resistance rates equivalent to Indian teak (Tectona grandis). In this study, the macromolecular constituents and total phenols compounds as well as the antioxidant and antibacterial activities of this wood were evaluated. Total phenols and proanthocyanidin levels were higher in wood when compared with bark levels. The antioxidant activity of wood extracts (IC50 = 18.7 μg/mL) was more effective than that of bark extracts (IC50 = 20.9 μg/mL). Wood and bark extracts revealed a high potential for inhibition of aerobic and anaerobic bacteria. The bark extracts were the most active (MIC from 20 to 60 μg/mL). Both antioxidant activity and high potential for bacteria inhibition turn these extracts promising for drug formulations, especially as antibacterial agent. PMID:22454666

  12. Establishing the tolerability and performance of tamarind seed polysaccharide (TSP) in treating dry eye syndrome: results of a clinical study.

    PubMed

    Rolando, Maurizio; Valente, Cristiana

    2007-03-29

    One of the problems arising from available preparations for dry eye syndrome is the limited residence time of products on the ocular surface. In this paper, we look at an innovative new treatment for dry eye, tamarind seed polysaccharide (TSP). TSP possesses mucomimetic, mucoadhesive and pseudoplastic properties. The 'mucin-like' molecular structure of TSP is similar to corneal and conjunctival mucin 1 (MUC1), a transmembrane glycoprotein thought to play an essential role in protecting and wetting the corneal surface and may explain its increased retention on the eye surface. The activity of TSP and hyaluronic acid (HA) in the treatment of dry eye syndrome was compared in an open-label, randomised, single-centre clinical study. Thirty patients were randomised to receive three or more applications per day of either TSP 0.5%, TSP 1% or HA 0.2% (Hyalistiltrade mark) over a period of 90 days. The primary objective of tolerability was assessed by visual analogue scale (VAS), scoring of specific symptoms and the incidence of adverse events. Secondary objectives included improvement in stability of the precorneal tear film, subjective symptoms and corneal and conjunctival staining. TSP 0.5% and 1% were comparable to HA 0.2% with regard to both primary and secondary objective parameters.TSP 1% showed benefits over HA 0.2% for the subjective symptoms; trouble blinking, ocular burning and foreign body sensation. This study suggests that TSP 0.5% and 1% offer at least equivalent relief to HA 0.2% for dry eye syndrome. All treatments demonstrated optimal tolerability and are suitable for frequent use in the therapy of dry eye.TSP 1% produced promising results in terms of improvements in certain patient symptoms and suggests benefits of the TSP formulation. This study paves the way for a larger study to further establish the performance and safety of TSP compared with HA and highlights the need to expand this therapeutic agent to a wider dry eye population.

  13. Physiological Effects of Touching Coated Wood.

    PubMed

    Ikei, Harumi; Song, Chorong; Miyazaki, Yoshifumi

    2017-07-13

    This study examined the physiological effects of touching wood with various coating with the palm of the hand on brain activity and autonomic nervous activity. Participants were 18 female university students (mean age, 21.7 ± 1.6 years). As an indicator of brain activity, oxyhemoglobin concentrations were measured in the left and right prefrontal cortices using near-infrared time-resolved spectroscopy. Heart rate variability (HRV) and heart rate were used as indicators of autonomic nervous activity. The high-frequency (HF) component of HRV, which reflects parasympathetic nervous activity, and the low-frequency (LF)/HF ratio, which reflects sympathetic nervous activity, were measured. Plates of uncoated, oil-finished, vitreous-finished, urethane-finished, and mirror-finished white oak wood were used as tactile stimuli. After sitting at rest with their eyes closed for 60 s, participants touched the stimuli with their palm for 90 s each. The results indicated that tactile stimulation with uncoated wood calmed prefrontal cortex activity (vs. urethane finish and mirror finish), increased parasympathetic nervous activity (vs. vitreous finish, urethane finish, and mirror finish), and decreased heart rate (vs. mirror finish), demonstrating a physiological relaxation effect. Further, tactile stimulation with oil- and vitreous-finished wood calmed left prefrontal cortex activity and decreased heart rate relative to mirror-finished wood.

  14. Physiological Effects of Touching Coated Wood

    PubMed Central

    2017-01-01

    This study examined the physiological effects of touching wood with various coating with the palm of the hand on brain activity and autonomic nervous activity. Participants were 18 female university students (mean age, 21.7 ± 1.6 years). As an indicator of brain activity, oxyhemoglobin concentrations were measured in the left and right prefrontal cortices using near-infrared time-resolved spectroscopy. Heart rate variability (HRV) and heart rate were used as indicators of autonomic nervous activity. The high-frequency (HF) component of HRV, which reflects parasympathetic nervous activity, and the low-frequency (LF)/HF ratio, which reflects sympathetic nervous activity, were measured. Plates of uncoated, oil-finished, vitreous-finished, urethane-finished, and mirror-finished white oak wood were used as tactile stimuli. After sitting at rest with their eyes closed for 60 s, participants touched the stimuli with their palm for 90 s each. The results indicated that tactile stimulation with uncoated wood calmed prefrontal cortex activity (vs. urethane finish and mirror finish), increased parasympathetic nervous activity (vs. vitreous finish, urethane finish, and mirror finish), and decreased heart rate (vs. mirror finish), demonstrating a physiological relaxation effect. Further, tactile stimulation with oil- and vitreous-finished wood calmed left prefrontal cortex activity and decreased heart rate relative to mirror-finished wood. PMID:28703777

  15. Knowledge, attitudes and practices in tamarind (Tamarindus indica L) use and conservation in Eastern Uganda.

    PubMed

    Ebifa-Othieno, Esther; Mugisha, Antony; Nyeko, Philip; Kabasa, John David

    2017-01-21

    Tamarindus indica L is one of the indigenous fruit tree species that traditionally contributes to food security and ecosystem stability in sub-Saharan Africa. We hypothesized that the indigenous people of Eastern Uganda have used T. indica for generations and developed practices that promote its conservation and therefore we expected that they possess an elaborate indigenous knowledge (IK) system and that most of them have planted the species. The aim of this study was to assess the influence of IK, attitudes and practices on the use and conservation of T. indica. A cross sectional survey was conducted in two districts purposively selected from the T. indica natural range. Focus group discussions, key informant interviews, semi-structured interviews and observation were used to collect data. Data was processed using qualitative analytical methods. Tamarindus indica was highly valued by the majority of the population. Tamarindus indica was used for food, medicinal, cultural, social, environmental amelioration and income generation purposes. The population possessed a high level of IK about T. indica evidenced by 18 categories of uses and multiple modes of use. Fruit pulp was the most commonly used tamarind product. Relative frequency of citation of the different uses provides insight into usage levels and IK possessed. The communities' food and medicinal uses concurred with scientific reports of health benefits of consuming T. indica. Approximately half of respondents had T. indica on their compounds or homegardens (53%). Fifty two percent of the tamarind population was self-propagated, 45% were planted while the propagation history for the remaining 3% was not known. Constraints towards planting T. indica included limited land, long maturation period and low monetary value. Fifty three percent of those who were growing T. indica did not carry out any silvicultural practices. The majority of T. indica encountered (87%) was intercropped with other crops or trees. Several beliefs and taboos regarding T. indica persist. Tamarindus indica has a high use value in the study area evidenced by multiple uses. Indigenous knowledge and uses concurred with scientifically proven nutritional and medicinal attributes of T. indica in literature which is significant given current trends towards affordable functional foods. The high level of IK has not translated into high rates of planting T. indica. There is need to encourage value-addition so as to maximize T. indica benefits and enhance conservation.

  16. The genotoxic contribution of wood smoke to indoor respirable suspended particles

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Boone, P.M.; Rossman, T.G.; Daisey, J.M.

    1989-01-01

    The effect of wood burning stoves on the genotoxicity of indoor respirable organic matter was investigated for four homes during the winter and spring of 1986. Paired samples, one collected when the stove was not used and one when wood was burned, were extracted with dichloromethane and acetone. Aliquots of the dichloromethane extracts were analyzed with and without metabolic activation using the Microscreen bioassay. The Microscreen is a rapid, sensitive bioassay which measures a broad genotoxic endpoint, {lambda}-prophage induction. Per nanogram of organic material, wood smoke proved to be a major source of indirect (observed with metabolic activation) but notmore » direct genotoxins in homes. The increase in indirect genotoxicity for extracts from aerosol containing wood smoke is probably due to higher concentrations of polycyclic aromatic hydrocarbons in the wood smoke aerosol as well as other unidentified classes. The direct genotoxicity observed for extracts of aerosol not containing wood smoke decreased with metabolic activation. This direct genotoxicity may be related to cooking activities in the homes. The trends in genotoxicity observed per nanogram of organic material are more pronounced when expressed per m{sup 3} of air due to the higher percentage of extractable material in aerosol containing wood smoke.« less

  17. Formulation of olfactory-targeted microparticles with tamarind seed polysaccharide to improve nose-to-brain transport of drugs.

    PubMed

    Yarragudi, Sasi B; Richter, Robert; Lee, Helen; Walker, Greg F; Clarkson, Andrew N; Kumar, Haribalan; Rizwan, Shakila B

    2017-05-01

    Targeted delivery and retention of drug formulations in the olfactory mucosa, the target site for nose-to-brain drug absorption is a major challenge due to the geometrical complexity of the nose and nasal clearance. Recent modelling data indicates that 10μm-sized microparticles show maximum deposition in the olfactory mucosa. In the present study we tested the hypothesis that 10μm-sized mucoadhesive microparticles would preferentially deposit on, and increase retention of drug on, the olfactory mucosa in a novel 3D-printed human nasal-replica cast under simulated breathing. The naturally occurring mucoadhesive polymer, tamarind seed polysaccharide (TSP) was used to formulate the microparticles using a spray drying technique. Physicochemical properties of microparticles such as size, morphology and mucoadhesiveness was investigated using a combination of laser diffraction, electron microscopy and texture-analysis. Furthermore, FITC-dextrans (5-40kDa) were incorporated in TSP-microparticles as model drugs. Size-dependent permeability of the FITC-dextrans was observed ex vivo using porcine nasal mucosa. Using the human nasal-replica cast, greater deposition of 10μm TSP-microparticles in the olfactory region was observed compared to TSP-microparticles 2μm in size. Collectively, these findings support our hypothesis that 10μm-sized mucoadhesive microparticles can achieve selective deposition and retention of drug in the olfactory mucosa. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. A Trypsin Inhibitor from Tamarind Reduces Food Intake and Improves Inflammatory Status in Rats with Metabolic Syndrome Regardless of Weight Loss.

    PubMed

    Carvalho, Fabiana M C; Lima, Vanessa C O; Costa, Izael S; Medeiros, Amanda F; Serquiz, Alexandre C; Lima, Maíra C J S; Serquiz, Raphael P; Maciel, Bruna L L; Uchôa, Adriana F; Santos, Elizeu A; Morais, Ana H A

    2016-09-27

    Trypsin inhibitors are studied in a variety of models for their anti-obesity and anti-inflammatory bioactive properties. Our group has previously demonstrated the satietogenic effect of tamarind seed trypsin inhibitors (TTI) in eutrophic mouse models and anti-inflammatory effects of other trypsin inhibitors. In this study, we evaluated TTI effect upon satiety, biochemical and inflammatory parameters in an experimental model of metabolic syndrome (MetS). Three groups of n = 5 male Wistar rats with obesity-based MetS received for 10 days one of the following: (1) Cafeteria diet; (2) Cafeteria diet + TTI (25 mg/kg); and (3) Standard diet. TTI reduced food intake in animals with MetS. Nevertheless, weight gain was not different between studied groups. Dyslipidemia parameters were not different with the use of TTI, only the group receiving standard diet showed lower very low density lipoprotein (VLDL) and triglycerides (TG) (Kruskal-Wallis, p < 0.05). Interleukin-6 (IL-6) production did not differ between groups. Interestingly, tumor necrosis factor-alpha (TNF-α) was lower in animals receiving TTI. Our results corroborate the satietogenic effect of TTI in a MetS model. Furthermore, we showed that TTI added to a cafeteria diet may decrease inflammation regardless of weight loss. This puts TTI as a candidate for studies to test its effectiveness as an adjuvant in MetS treatment.

  19. A Trypsin Inhibitor from Tamarind Reduces Food Intake and Improves Inflammatory Status in Rats with Metabolic Syndrome Regardless of Weight Loss

    PubMed Central

    Carvalho, Fabiana M. C.; Lima, Vanessa C. O.; Costa, Izael S.; Medeiros, Amanda F.; Serquiz, Alexandre C.; Lima, Maíra C. J. S.; Serquiz, Raphael P.; Maciel, Bruna L. L.; Uchôa, Adriana F.; Santos, Elizeu A.; Morais, Ana H. A.

    2016-01-01

    Trypsin inhibitors are studied in a variety of models for their anti-obesity and anti-inflammatory bioactive properties. Our group has previously demonstrated the satietogenic effect of tamarind seed trypsin inhibitors (TTI) in eutrophic mouse models and anti-inflammatory effects of other trypsin inhibitors. In this study, we evaluated TTI effect upon satiety, biochemical and inflammatory parameters in an experimental model of metabolic syndrome (MetS). Three groups of n = 5 male Wistar rats with obesity-based MetS received for 10 days one of the following: (1) Cafeteria diet; (2) Cafeteria diet + TTI (25 mg/kg); and (3) Standard diet. TTI reduced food intake in animals with MetS. Nevertheless, weight gain was not different between studied groups. Dyslipidemia parameters were not different with the use of TTI, only the group receiving standard diet showed lower very low density lipoprotein (VLDL) and triglycerides (TG) (Kruskal–Wallis, p < 0.05). Interleukin-6 (IL-6) production did not differ between groups. Interestingly, tumor necrosis factor-alpha (TNF-α) was lower in animals receiving TTI. Our results corroborate the satietogenic effect of TTI in a MetS model. Furthermore, we showed that TTI added to a cafeteria diet may decrease inflammation regardless of weight loss. This puts TTI as a candidate for studies to test its effectiveness as an adjuvant in MetS treatment. PMID:27690087

  20. Effect of Extraction Conditions on the Antioxidant Activity of Olive Wood Extracts

    PubMed Central

    Pérez-Bonilla, Mercedes; Salido, Sofía; Sánchez, Adolfo; van Beek, Teris A.; Altarejos, Joaquín

    2013-01-01

    An investigation to optimize the extraction yield and the radical scavenging activity from the agricultural by-product olive tree wood (Olea europaea L., cultivar Picual) using six different extraction protocols was carried out. Four olive wood samples from different geographical origin, and harvesting time have been used for comparison purposes. Among the fifty olive wood extracts obtained in this study, the most active ones were those prepared with ethyl acetate, either through direct extraction or by successive liquid-liquid partitioning procedures, the main components being the secoiridoids oleuropein and ligustroside. An acid hydrolysis pretreatment of olive wood samples before extractions did not improve the results. In the course of this study, two compounds were isolated from the ethanolic extracts of olive wood collected during the olives' harvesting season and identified as (7′′R)-7′′-ethoxyoleuropein (1) and (7′′S)-7′′-ethoxyoleuropein (2). PMID:26904608

  1. 75 FR 20838 - Agency Information Collection Activities; Submission to OMB for Review and Approval; Comment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-21

    ... Collection Activities; Submission to OMB for Review and Approval; Comment Request; NESHAP for Wood Furniture... electronic docket, go to http://www.regulations.gov . Title: NESHAP for Wood Furniture Manufacturing.... Respondents/Affected Entities: Wood furniture manufacturing operations. Estimated Number of Respondents: 750...

  2. 75 FR 62384 - Agency Information Collection Activities; Submission to OMB for Review and Approval; Comment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-08

    ... Activities; Submission to OMB for Review and Approval; Comment Request; NSPS for New Residential Wood Heaters...://www.regulations.gov . Title: NSPS for New Residential Wood Heaters (Renewal). ICR Numbers: EPA ICR.... Abstract: The New Source Performance Standard for New Residential Wood Heaters was proposed on February 18...

  3. 77 FR 58538 - Agency Information Collection Activities; Submission to OMB for Review and Approval; Comment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-21

    ... Activities; Submission to OMB for Review and Approval; Comment Request; NESHAP for the Wood Building Products...: NESHAP for the Wood Building Products Surface Coating Industry (Renewal). ICR Numbers: EPA ICR Number... transmit or otherwise disclose the information. Respondents/Affected Entities: Owners or operators of wood...

  4. Recycling of wood for particle board production: accounting of greenhouse gases and global warming contributions.

    PubMed

    Merrild, Hanna; Christensen, Thomas H

    2009-11-01

    The greenhouse gas (GHG) emissions related to the recycling of wood waste have been assessed with the purpose to provide useful data that can be used in accounting of greenhouse gas emissions. Here we present data related to the activities in a material recovery facility (MRF) where wood waste is shredded and foreign objects are removed in order to produce wood chips for use in the production of particleboard. The data are presented in accordance with the UOD (upstream, operational, downstream) framework presented in Gentil et al. (Waste Management & Research, 27, 2009). The GHG accounting shows that the emissions related to upstream activities (5 to 41 kg CO(2)-equivalents tonne( -1) wood waste) and to activities at the MRF (approximately 5 kg CO(2)-equivalents tonne(-1) wood waste) are negligible compared to the downstream processing (-560 to -120 kg CO(2)equivalents tonne(-1) wood waste). The magnitude of the savings in GHG emissions downstream are mainly related to savings in energy consumption for drying of fresh wood for particleboard production. However, the GHG account highly depends on the choices made in the modelling of the downstream system. The inclusion of saved electricity from avoided chipping of virgin wood does not change the results radically (-665 to -125 kg CO(2)-equivalents tonne(- 1) wood waste). However, if in addition it is assumed that the GHG emissions from combustion of wood has no global warming potential (GWP) and that the energy produced from excess wood due to recycling substitutes energy from fossil fuels, here assumed to be coal, potentially large downstream GHG emissions savings can be achieved by recycling of waste wood (-1.9 to -1.3 tonnes CO(2)-equivalents tonne(- 1) wood waste). As the data ranges are broad, it is necessary to carefully evaluate the feasibility of the data in the specific system which the GHG accounting is to be applied to.

  5. Patterns of lignin degradation and oxidative enzyme secretion by different wood- and litter-colonizing basidiomycetes and ascomycetes grown on beech-wood.

    PubMed

    Liers, Christiane; Arnstadt, Tobias; Ullrich, René; Hofrichter, Martin

    2011-10-01

    The degradation of lignocellulose and the secretion of extracellular oxidoreductases were investigated in beech-wood (Fagus sylvatica) microcosms using 11 representative fungi of four different ecophysiological and taxonomic groups causing: (1) classic white rot of wood (e.g. Phlebia radiata), (2) 'nonspecific' wood rot (e.g. Agrocybe aegerita), (3) white rot of leaf litter (Stropharia rugosoannulata) or (4) soft rot of wood (e.g. Xylaria polymorpha). All strong white rotters produced manganese-oxidizing peroxidases as the key enzymes of ligninolysis (75-2200 mU g(-1)), whereas lignin peroxidase activity was not detectable in the wood extracts. Interestingly, activities of two recently discovered peroxidases - aromatic peroxygenase and a manganese-independent peroxidase of the DyP-type - were detected in the culture extracts of A. aegerita (up to 125 mU g(-1)) and Auricularia auricula-judae (up to 400 mU g(-1)), respectively. The activity of classic peroxidases correlated to some extent with the removal of wood components (e.g. Klason lignin) and the release of small water-soluble fragments (0.5-1.0 kDa) characterized by aromatic constituents. In contrast, laccase activity correlated with the formation of high-molecular mass fragments (30-200 kDa). The differences observed in the degradation patterns allow to distinguish the rot types caused by basidiomycetes and ascomycetes and may be suitable for following the effects of oxidative key enzymes (ligninolytic peroxidases vs. laccases, role of novel peroxidases) during wood decay. © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  6. The central role of wood biology in understanding the durability of wood-coating interactions

    Treesearch

    Alex C. Wiedenhoeft

    2007-01-01

    To design effectively for durability, one must actively and honestly assess the material properties and limitations of each of the components in the design system; wood or wood composite, and the coating. Inasmuch as wood coatings are manufactured to specified tolerances from known materials, we have control of that component of the system. Compared to manmade...

  7. Domestic market activity in solid wood products in the United States, 1950-1998.

    Treesearch

    David B. McKeever

    2002-01-01

    Solid wood is important to the construction, manufacturing, and shipping segments of the U.S. economy. Nearly all new houses are built with wood, and wood building products are used in the construction of nonresidential buildings, and in the upkeep and improvement of existing structures. Solid wood is used extensively to produce and transport manufactured products. It...

  8. Climate Change Mitigation Challenge for Wood Utilization-The Case of Finland.

    PubMed

    Soimakallio, Sampo; Saikku, Laura; Valsta, Lauri; Pingoud, Kim

    2016-05-17

    The urgent need to mitigate climate change invokes both opportunities and challenges for forest biomass utilization. Fossil fuels can be substituted by using wood products in place of alternative materials and energy, but wood harvesting reduces forest carbon sink and processing of wood products requires material and energy inputs. We assessed the extended life cycle carbon emissions considering substitution impacts for various wood utilization scenarios over 100 years from 2010 onward for Finland. The scenarios were based on various but constant wood utilization structures reflecting current and anticipated mix of wood utilization activities. We applied stochastic simulation to deal with the uncertainty in a number of input variables required. According to our analysis, the wood utilization decrease net carbon emissions with a probability lower than 40% for each of the studied scenarios. Furthermore, large emission reductions were exceptionally unlikely. The uncertainty of the results were influenced clearly the most by the reduction in the forest carbon sink. There is a significant trade-off between avoiding emissions through fossil fuel substitution and reduction in forest carbon sink due to wood harvesting. This creates a major challenge for forest management practices and wood utilization activities in responding to ambitious climate change mitigation targets.

  9. Downregulation of RWA genes in hybrid aspen affects xylan acetylation and wood saccharification.

    PubMed

    Pawar, Prashant Mohan-Anupama; Ratke, Christine; Balasubramanian, Vimal K; Chong, Sun-Li; Gandla, Madhavi Latha; Adriasola, Mathilda; Sparrman, Tobias; Hedenström, Mattias; Szwaj, Klaudia; Derba-Maceluch, Marta; Gaertner, Cyril; Mouille, Gregory; Ezcurra, Ines; Tenkanen, Maija; Jönsson, Leif J; Mellerowicz, Ewa J

    2017-06-01

    High acetylation of angiosperm wood hinders its conversion to sugars by glycoside hydrolases, subsequent ethanol fermentation and (hence) its use for biofuel production. We studied the REDUCED WALL ACETYLATION (RWA) gene family of the hardwood model Populus to evaluate its potential for improving saccharification. The family has two clades, AB and CD, containing two genes each. All four genes are expressed in developing wood but only RWA-A and -B are activated by master switches of the secondary cell wall PtNST1 and PtMYB21. Histochemical analysis of promoter::GUS lines in hybrid aspen (Populus tremula × tremuloides) showed activation of RWA-A and -B promoters in the secondary wall formation zone, while RWA-C and -D promoter activity was diffuse. Ectopic downregulation of either clade reduced wood xylan and xyloglucan acetylation. Suppressing both clades simultaneously using the wood-specific promoter reduced wood acetylation by 25% and decreased acetylation at position 2 of Xylp in the dimethyl sulfoxide-extracted xylan. This did not affect plant growth but decreased xylose and increased glucose contents in the noncellulosic monosaccharide fraction, and increased glucose and xylose yields of wood enzymatic hydrolysis without pretreatment. Both RWA clades regulate wood xylan acetylation in aspen and are promising targets to improve wood saccharification. © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

  10. Cytosolic invertase contributes to the supply of substrate for cellulose biosynthesis in developing wood.

    PubMed

    Rende, Umut; Wang, Wei; Gandla, Madhavi Latha; Jönsson, Leif J; Niittylä, Totte

    2017-04-01

    Carbon for cellulose biosynthesis is derived from sucrose. Cellulose is synthesized from uridine 5'-diphosphoglucose (UDP-glucose), but the enzyme(s) responsible for the initial sucrose cleavage and the source of UDP-glucose for cellulose biosynthesis in developing wood have not been defined. We investigated the role of CYTOSOLIC INVERTASEs (CINs) during wood formation in hybrid aspen (Populus tremula × tremuloides) and characterized transgenic lines with reduced CIN activity during secondary cell wall biosynthesis. Suppression of CIN activity by 38-55% led to a 9-13% reduction in crystalline cellulose. The changes in cellulose were reflected in reduced diameter of acid-insoluble cellulose microfibrils and increased glucose release from wood upon enzymatic digestion of cellulose. Reduced CIN activity decreased the amount of the cellulose biosynthesis precursor UDP-glucose in developing wood, pointing to the likely cause of the cellulose phenotype. The findings suggest that CIN activity has an important role in the cellulose biosynthesis of trees, and indicate that cellulose biosynthesis in wood relies on a quantifiable UDP-glucose pool. The results also introduce a concept of altering cellulose microfibril properties by modifying substrate supply to cellulose biosynthesis. © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

  11. Metabolite secretion, Fe(3+)-reducing activity and wood degradation by the white-rot fungus Trametes versicolor ATCC 20869.

    PubMed

    Aguiar, André; Gavioli, Daniela; Ferraz, André

    2014-11-01

    Trametes versicolor is a promising white-rot fungus for the biological pretreatment of lignocellulosic biomass. In the present work, T. versicolor ATCC 20869 was grown on Pinus taeda wood chips under solid-state fermentation conditions to examine the wood-degrading mechanisms employed by this fungus. Samples that were subjected to fungal pretreatment for one-, two- and four-week periods were investigated. The average mass loss ranged from 5 % to 8 % (m m(-)(1)). The polysaccharides were preferentially degraded: hemicellulose and glucan losses reached 13.4 % and 6.9 % (m m(-)(1)) after four weeks of cultivation, respectively. Crude enzyme extracts were obtained and assayed using specific substrates and their enzymatic activities were measured. Xylanases were the predominant enzymes, while cellobiohydrolase activities were marginally detected. Endoglucanase activity, β-glucosidase activity, and wood glucan losses increased up to the second week of biodegradation and remained constant after that time. Although no lignin-degrading enzyme activity was detected, the lignin loss reached 7.5 % (m m(-)(1)). Soluble oxalic acid was detected in trace quantities. After the first week of biodegradation, the Fe(3+)-reducing activity steadily increased with time, but the activity levels were always lower than those observed in the undecayed wood. The progressive wood polymer degradation appeared related to the secretion of hydrolytic enzymes, as well as to Fe(3+)-reducing activity, which was restored in the cultures after the first week of biodegradation. Copyright © 2014 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

  12. Hydroxyethyl methacrylate grafted carboxy methyl tamarind (CMT-g-HEMA) polysaccharide based matrix as a suitable scaffold for skin tissue engineering.

    PubMed

    Choudhury, Priyanka; Kumar, Satish; Singh, Abhishek; Kumar, Ashutosh; Kaur, Navneet; Sanyasi, Sridhar; Chawla, Saurabh; Goswami, Chandan; Goswami, Luna

    2018-06-01

    Patho-physiologies related to skin are diverse in nature such as burns, skin ulcers, atopic dermatitis, psoriasis etc. which impose severe bio-medical problems and thus enforce requirement of new and healthy skin prepared through tissues engineering methodologies. However, fully functional and biodegradable matrix for attachment, growth, proliferation and differentiation of the relevant cells is not available. In the present study, we introduce a set of hydrogels synthesized by incorporation of a synthetic monomer (Hydroxyethlmethacryate) with a semi-synthetic polymer backbone (carboxy methyl tamarind, CMT) in different mole ratios. We termed these materials as CMT:HEMA based hydrogels and these were characterized by different physico-chemical techniques, namely by X-Ray Diffraction, SEM and Dynamic Light Scattering. Biocompatibility studies with HaCaT, NIH-3T3 and mouse dermal fibroblasts confirm that this material is biocompatible. MTT assay further confirmed that this material does not have any cytotoxic effects. Assays for mitochondrial functionality such as ATP assay and mitochondrial reactive oxygen (ROS) generation also suggest that this material is safe and does not have any cytotoxicity. Hemolytic assay with red blood cells and acute skin irritation test on SD Rats confirmed that this material is suitable for ex-vivo application in future. We suggest that this hydrogel is suitable for in-vivo applications and may have clinical and commercial importance against skin disorders. Copyright © 2018. Published by Elsevier Ltd.

  13. Development of aprepitant loaded orally disintegrating films for enhanced pharmacokinetic performance.

    PubMed

    Sharma, Radhika; Kamboj, Sunil; Singh, Gursharan; Rana, Vikas

    2016-03-10

    The present investigation was aimed to prepare orally disintegrating films (ODFs) containing aprepitant (APT), an antiemetic drug employing pullulan as film forming agent, tamarind pectin as wetting agent and liquid glucose as plasticizer and solubiliser. The ODFs were prepared using solvent casting method. The method was optimized employing 3(2) full factorial design considering proportion of pullulan: tamarind pectin and concentration of liquid glucose as independent variables and disintegration time, wetting time, folding endurance, tensile strength and extensibility as dependent variables. The optimized ODF was evaluated for various physicochemical, mechanical, drug release kinetics and bioavailability studies. The results suggested prepared film has uniform film surface, non-sticky and disintegrated within 18s. The in-vitro release kinetics revealed more than 87% aprepitant was released from optimized ODF as compared to 85%, 49%, and 12% aprepitant release from marketed formulation Aprecap, micronized aprepitant and non micronized aprepitant, respectively. The results of animal preference study indicated that developed aprepitant loaded ODFs are accepted by rabbits as food material. Animal pharmacokinetic (PK) study showed 1.80, 1.56 and 1.36 fold enhancement in relative bioavailability for aprepitant loaded ODF, Aprecap and micronized aprepitant respectively, in comparison with non-micronized aprepitant. Overall, the solubilised aprepitant when incorporated in the form of aprepitant loaded ODF showed enhanced bioavailability as compared to micronized/non-micronized aprepitant based oral formulations. These findings suggested that aprepitant loaded ODF could be effective for antiemesis during cancer chemotherapy. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. Fabrication and characterization of nanoengineered biocompatible n-HA/chitosan-tamarind seed polysaccharide: Bio-inspired nanocomposites for bone tissue engineering.

    PubMed

    Shakir, Mohammad; Zia, Iram; Rehman, Abdur; Ullah, Rizwan

    2018-05-01

    In this communication we describe the fabrication of nano-hydroxyapatite/chitosan-tamarind seed polysaccharide (n-HA/CS-TSP) nanocomposite with a weight ratio of 70/20/10, 70/15/15 and 70/10/20, respectively through a co-precipitation method. A comparative assessment of the properties of n-HA/CS-TSP and n-HA/CS nanocomposites was done by FT-IR, SEM-EDX, TEM, TGA/DTA, XRD and mechanical testing. The results suggested strong chemical interactions between the three components, decreased particle size and homogeneous dispersion of n-HA particles in n-HA/CS-TSP as compared to n-HA/CS. The n-HA/CS-TSP (70/10/20) showed the most porous and rough surface, enhanced thermal stability and highest compressive strength (4.0 MPa) and modulus (81 MPa). In addition, n-HA/CS-TSP (70/10/20) exhibited greater swelling character, acceptable degradation and increased biomineralization in simulated body fluid (SBF) as compared to n-HA/CS-TSP (70/20/10, 70/15/15) and n-HA/CS nanocomposites. The superior non-toxic response with MG-63 cells and better haemocompatibility was observed with n-HA/CS-TSP (70/15/15). Thereby, n-HA/CS-TSP nanocomposites could be promising alternative biomaterials in the field of bone tissue engineering compared to the n-HA/CS nanocomposite. Copyright © 2018 Elsevier B.V. All rights reserved.

  15. Effect of Tamarindus indica leaf powder on plasma concentrations of copper, zinc, and iron in fluorotic cows.

    PubMed

    Samal, Pinaki; Patra, R C; Gupta, A R; Mishra, S K; Jena, D; Satapathy, D

    2016-10-01

    The main objective of the study was to determine the deleterious effect of fluoride on plasma trace minerals of fluorotic cattle and to evaluate the effect of Tamarindus indica leaf powder toward correction of the same. A total of 30 cattle exhibiting chronic sign of fluorosis and 10 healthy cattle from nonfluorotic area were incorporated in this study. Fluorotic cattle were divided into three equal groups consisting of 10 cattle each. Group I from fluoride free area served as healthy control. The Group II received no treatment and served as disease control. Groups III and IV were supplemented with tamarind leaf powder at 15 g and 30 g/day with feed for 60 days. Plasma mineral status was evaluated after 60 days of treatment with double beam atomic absorption spectrophotometer. Statistical analysis of data revealed a significant (p<0.05) decrease in mean plasma copper (Cu) (0.344±0.007 ppm), zinc (Zn) (0.692±0.06 ppm), and iron (Fe) concentration (1.100±0.01 ppm) in fluorotic cattle in comparison to healthy cattle (0.58±0.010, 2.342±0.04, 1.406±0.04 ppm, respectively). Significant (p<0.05) increase in Cu, Zn, and Fe was recorded after supplementation of tamarind leaf powder to the fluorotic cattle. It was concluded that fluorotic cattle might be supplemented with T. indica leaf powder with feed for the correction of the decreased level of certain plasma minerals.

  16. Aspen SUCROSE TRANSPORTER3 Allocates Carbon into Wood Fibers1[C][W

    PubMed Central

    Mahboubi, Amir; Ratke, Christine; Gorzsás, András; Kumar, Manoj; Mellerowicz, Ewa J.; Niittylä, Totte

    2013-01-01

    Wood formation in trees requires carbon import from the photosynthetic tissues. In several tree species, including Populus species, the majority of this carbon is derived from sucrose (Suc) transported in the phloem. The mechanism of radial Suc transport from phloem to developing wood is not well understood. We investigated the role of active Suc transport during secondary cell wall formation in hybrid aspen (Populus tremula × Populus tremuloides). We show that RNA interference-mediated reduction of PttSUT3 (for Suc/H+ symporter) during secondary cell wall formation in developing wood caused thinner wood fiber walls accompanied by a reduction in cellulose and an increase in lignin. Suc content in the phloem and developing wood was not significantly changed. However, after 13CO2 assimilation, the SUT3RNAi lines contained more 13C than the wild type in the Suc-containing extract of developing wood. Hence, Suc was transported into developing wood, but the Suc-derived carbon was not efficiently incorporated to wood fiber walls. A yellow fluorescent protein:PttSUT3 fusion localized to plasma membrane, suggesting that reduced Suc import into developing wood fibers was the cause of the observed cell wall phenotype. The results show the importance of active Suc transport for wood formation in a symplasmically phloem-loading tree species and identify PttSUT3 as a principal transporter for carbon delivery into secondary cell wall-forming wood fibers. PMID:24170204

  17. Activation of Transient Receptor Potential Ankyrin-1 (TRPA1) in Lung Cells by Wood Smoke Particulate Material

    PubMed Central

    Shapiro, Darien; Deering-Rice, Cassandra E.; Romero, Erin G.; Hughen, Ronald W.; Light, Alan R.; Veranth, John M.; Reilly, Christopher A.

    2013-01-01

    Cigarette smoke, diesel exhaust, and other combustion-derived particles activate the calcium channel transient receptor potential ankyrin-1 (TRPA1), causing irritation and inflammation in the respiratory tract. It was hypothesized that wood smoke particulate and select chemical constituents thereof would also activate TRPA1 in lung cells, potentially explaining the adverse effects of wood and other forms of biomass smoke on the respiratory system. TRPA1 activation was assessed using calcium imaging assays in TRPA1-overexpressing HEK-293 cells, mouse primary trigeminal neurons, and human adenocarcinoma (A549) lung cells. Particles from pine and mesquite smoke were less potent agonists of TRPA1 than an equivalent mass concentration of an ethanol extract of diesel exhaust particles; pine particles were comparable in potency to cigarette smoke condensate, and mesquite particles were the least potent. The fine particulate (PM<2.5 μm) of wood smoke were the most potent TRPA1 agonists and several chemical constituents of wood smoke particulate: 3,5-ditert-butylphenol, coniferaldehyde, formaldehyde, perinaphthenone, agathic acid, and isocupressic acid were TRPA1 agonists. Pine particulate activated TRPA1 in mouse trigeminal neurons and A549 cells in a concentration-dependent manner, which was inhibited by the TRPA1 antagonist HC-030031. TRPA1 activation by wood smoke particles occurred through the electrophile/oxidant-sensing domain (i.e., C621/C641/C665/K710), based on the inhibition of cellular responses when the particles were pre-treated with glutathione; a role for the menthol-binding site of TRPA1 (S873/T874) was demonstrated for 3,5-ditert-butylphenol. This study demonstrated that TRPA1 is a molecular sensor for wood smoke particulate and several chemical constituents thereof, in sensory neurons and A549 cells, suggesting that TRPA1 may mediate some of the adverse effects of wood smoke in humans. PMID:23541125

  18. Nematicidal activity of plant essential oils and components from coriander (Coriandrum sativum), Oriental sweetgum (Liquidambar orientalis), and valerian (Valeriana wallichii) essential oils against pine wood nematode (Bursaphelenchus xylophilus).

    PubMed

    Kim, Junheon; Seo, Sun-Mi; Lee, Sang-Gil; Shin, Sang-Chul; Park, Il-Kwon

    2008-08-27

    Commercial essential oils from 28 plant species were tested for their nematicidal activities against the pine wood nematode, Bursaphelenchus xylophilus. Good nematicidal activity against B. xylophilus was achieved with essential oils of coriander (Coriandrum sativum), Oriental sweetgum (Liquidambar orientalis), and valerian (Valeriana wallichii). Analysis by gas chromatography-mass spectrometry led to the identification of 26, 11, and 4 major compounds from coriander (Coriandrum sativum), Oriental sweetgum (Liquidambar orientalis), and valerian (Valeriana wallichii) oils, respectively. Compounds from each plant essential oil were tested individually for their nematicidal activities against the pine wood nematode. Among the compounds, benzaldehyde, trans-cinnamyl alcohol, cis-asarone, octanal, nonanal, decanal, trans-2-decenal, undecanal, dodecanal, decanol, and trans-2-decen-1-ol showed strong nematicidal activity. The essential oils described herein merit further study as potential nematicides against the pine wood nematode.

  19. Preparation and characterizations of activated carbon monolith from rubber wood and its effect on supercapacitor performances

    NASA Astrophysics Data System (ADS)

    Taer, E.; Taslim, R.; Deraman, M.

    2016-02-01

    Preparation of activated carbon monolith (ACM) from rubber wood was investigated. Two kind of preparation method were carried out by pre-carbonized of rubber wood saw dust and rubber wood material as it is naturally. The samples were prepared with pelletizing method and small cutting of rubber wood in cross sectional method. Both of samples were characterized by physical and electrochemical technique. The physical properties such as morphology and porosity were investigated. The electrochemical properties of both samples such as equivalent series resistances (ESR) and specific capacitances were also compared. In conclusion, this study showed that both of different preparation method would propose a simple method of ACM electrode preparation technique for supercapacitor applications.

  20. Wood and society

    Treesearch

    Christopher D. Risbrudt

    2005-01-01

    Forests, and the wood they produce, have played an important role in human activity since before recorded history. Indeed, one of the first major innovations of humankind was utilizing fire, fueled by wood, for cooking and heating. It is very likely that early hominids used wood fires for cooking as long as 1.5 million years ago (Clark and Harris 1985). Clear evidence...

  1. Time-scale dynamics of proteome and transcriptome of the white-rot fungus Phlebia radiata: growth on spruce wood and decay effect on lignocellulose.

    PubMed

    Kuuskeri, Jaana; Häkkinen, Mari; Laine, Pia; Smolander, Olli-Pekka; Tamene, Fitsum; Miettinen, Sini; Nousiainen, Paula; Kemell, Marianna; Auvinen, Petri; Lundell, Taina

    2016-01-01

    The white-rot Agaricomycetes species Phlebia radiata is an efficient wood-decaying fungus degrading all wood components, including cellulose, hemicellulose, and lignin. We cultivated P. radiata in solid state cultures on spruce wood, and extended the experiment to 6 weeks to gain more knowledge on the time-scale dynamics of protein expression upon growth and wood decay. Total proteome and transcriptome of P. radiata were analyzed by peptide LC-MS/MS and RNA sequencing at specific time points to study the enzymatic machinery on the fungus' natural growth substrate. According to proteomics analyses, several CAZy oxidoreductase class-II peroxidases with glyoxal and alcohol oxidases were the most abundant proteins produced on wood together with enzymes important for cellulose utilization, such as GH7 and GH6 cellobiohydrolases. Transcriptome additionally displayed expression of multiple AA9 lytic polysaccharide monooxygenases indicative of oxidative cleavage of wood carbohydrate polymers. Large differences were observed for individual protein quantities at specific time points, with a tendency of enhanced production of specific peroxidases on the first 2 weeks of growth on wood. Among the 10 class-II peroxidases, new MnP1-long, characterized MnP2-long and LiP3 were produced in high protein abundances, while LiP2 and LiP1 were upregulated at highest level as transcripts on wood together with the oxidases and one acetyl xylan esterase, implying their necessity as primary enzymes to function against coniferous wood lignin to gain carbohydrate accessibility and fungal growth. Majority of the CAZy encoding transcripts upregulated on spruce wood represented activities against plant cell wall and were identified in the proteome, comprising main activities of white-rot decay. Our data indicate significant changes in carbohydrate-active enzyme expression during the six-week surveillance of P. radiata growing on wood. Response to wood substrate is seen already during the first weeks. The immediate oxidative enzyme action on lignin and wood cell walls is supported by detected lignin substructure sidechain cleavages, release of phenolic units, and visual changes in xylem cell wall ultrastructure. This study contributes to increasing knowledge on fungal genetics and lignocellulose bioconversion pathways, allowing us to head for systems biology, development of biofuel production, and industrial applications on plant biomass utilizing wood-decay fungi.

  2. Chapter 1: Wood and Society

    Treesearch

    Chrisopher D. Risbrudt

    2013-01-01

    Forests, and the wood they produce, have played an important role in human activity since before recorded history. Indeed, one of the first major innovations was utilizing fire, fueled by wood, for cooking and heating. It is very likely that early hominids used wood fires for cooking, as long as 1.5 million years ago (Clark and Harris 1985). Clear evidence of this use...

  3. Disturbance legacies of historic tie-drives persistently alter geomorphology and large wood characteristics in headwater streams, southeast Wyoming

    Treesearch

    Claire M. Ruffing; Melinda D. Daniels; Kathleen A. Dwire

    2015-01-01

    Instream wood is recognized as an integral component of stream morphology in forested areas. However, few studies have evaluated the legacy effects of historic wood removal activities and associated impacts on channel morphology, contemporary wood loading, and recruitment. This study investigates the role of historic tie-driving, a widespread channel disturbance legacy...

  4. U.S. wood pallet material use trends

    Treesearch

    Philip A. Araman; Robert J. Bush; E.Bradley Hager

    2010-01-01

    A series of five studies conducted by the Virginia Tech Department of Wood Science and Forest Products, in collaboration with the USDA – Forest Service (Blacksburg, Virginia), have tracked activity in the U.S. wood pallet and container industry between 1992 and 2006. The studies determined trends in wood use and pallet production within the industry, both new and...

  5. Wood Pallets and Landfills - Status and Opportunities for Economic Recovery and Recycling

    Treesearch

    Philip A. Araman; Robert J. Bush; A.L. Hammett; E. Brad Hager

    1998-01-01

    Wood pallet recovery, repair, and recycling are sound environmental activities that can reduce both forest resource demands and waste in landfills. Our studies found that 6.16 million tons of wood pallets (or 223.6 million pallets) entered municipal solid waste (MSW) and construction and demolition (C&D) landfill facilities in 1995. At the same time, wood pallet...

  6. Material use and production changes in the U.S. wood pallet and container industry: 1992 to 2006

    Treesearch

    R. J. Bush; P. A. Araman

    2009-01-01

    A series of five studies conducted by the Virginia Tech Department of Wood Science and Forest Products, in collaboration with the USDA - Forest Service (Blacksburg, Virginia), have tracked activity in the U.S. wood pallet and container industry between 1992 and 2006. The studies documented trends in wood use and pallet production with in the industry, both new...

  7. Aspen Tension Wood Fibers Contain β-(1→4)-Galactans and Acidic Arabinogalactans Retained by Cellulose Microfibrils in Gelatinous Walls1[OPEN

    PubMed Central

    Gorshkova, Tatyana; Mokshina, Natalia; Chernova, Tatyana; Ibragimova, Nadezhda; Salnikov, Vadim; Mikshina, Polina; Tryfona, Theodora; Banasiak, Alicja; Immerzeel, Peter; Dupree, Paul; Mellerowicz, Ewa J.

    2015-01-01

    Contractile cell walls are found in various plant organs and tissues such as tendrils, contractile roots, and tension wood. The tension-generating mechanism is not known but is thought to involve special cell wall architecture. We previously postulated that tension could result from the entrapment of certain matrix polymers within cellulose microfibrils. As reported here, this hypothesis was corroborated by sequential extraction and analysis of cell wall polymers that are retained by cellulose microfibrils in tension wood and normal wood of hybrid aspen (Populus tremula × Populus tremuloides). β-(1→4)-Galactan and type II arabinogalactan were the main large matrix polymers retained by cellulose microfibrils that were specifically found in tension wood. Xyloglucan was detected mostly in oligomeric form in the alkali-labile fraction and was enriched in tension wood. β-(1→4)-Galactan and rhamnogalacturonan I backbone epitopes were localized in the gelatinous cell wall layer. Type II arabinogalactans retained by cellulose microfibrils had a higher content of (methyl)glucuronic acid and galactose in tension wood than in normal wood. Thus, β-(1→4)-galactan and a specialized form of type II arabinogalactan are trapped by cellulose microfibrils specifically in tension wood and, thus, are the main candidate polymers for the generation of tensional stresses by the entrapment mechanism. We also found high β-galactosidase activity accompanying tension wood differentiation and propose a testable hypothesis that such activity might regulate galactan entrapment and, thus, mechanical properties of cell walls in tension wood. PMID:26378099

  8. Aspen Tension Wood Fibers Contain β-(1---> 4)-Galactans and Acidic Arabinogalactans Retained by Cellulose Microfibrils in Gelatinous Walls.

    PubMed

    Gorshkova, Tatyana; Mokshina, Natalia; Chernova, Tatyana; Ibragimova, Nadezhda; Salnikov, Vadim; Mikshina, Polina; Tryfona, Theodora; Banasiak, Alicja; Immerzeel, Peter; Dupree, Paul; Mellerowicz, Ewa J

    2015-11-01

    Contractile cell walls are found in various plant organs and tissues such as tendrils, contractile roots, and tension wood. The tension-generating mechanism is not known but is thought to involve special cell wall architecture. We previously postulated that tension could result from the entrapment of certain matrix polymers within cellulose microfibrils. As reported here, this hypothesis was corroborated by sequential extraction and analysis of cell wall polymers that are retained by cellulose microfibrils in tension wood and normal wood of hybrid aspen (Populus tremula × Populus tremuloides). β-(1→4)-Galactan and type II arabinogalactan were the main large matrix polymers retained by cellulose microfibrils that were specifically found in tension wood. Xyloglucan was detected mostly in oligomeric form in the alkali-labile fraction and was enriched in tension wood. β-(1→4)-Galactan and rhamnogalacturonan I backbone epitopes were localized in the gelatinous cell wall layer. Type II arabinogalactans retained by cellulose microfibrils had a higher content of (methyl)glucuronic acid and galactose in tension wood than in normal wood. Thus, β-(1→4)-galactan and a specialized form of type II arabinogalactan are trapped by cellulose microfibrils specifically in tension wood and, thus, are the main candidate polymers for the generation of tensional stresses by the entrapment mechanism. We also found high β-galactosidase activity accompanying tension wood differentiation and propose a testable hypothesis that such activity might regulate galactan entrapment and, thus, mechanical properties of cell walls in tension wood. © 2015 American Society of Plant Biologists. All Rights Reserved.

  9. Not Just Lumber—Using Wood in the Sustainable Future of Materials, Chemicals, and Fuels

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jakes, Joseph E.; Arzola, Xavier; Bergman, Rick

    Forest-derived biomaterials can play an integral role in a sustainable and renewable future. Research across a range of disciplines is required to develop the knowledge necessary to overcome the challenges of incorporating more renewable forest resources in materials, chemicals, and fuels. We focus on wood specifically because in our view, better characterization of wood as a raw material and as a feedstock will lead to its increased utilization. We first give an overview of wood structure and chemical composition and then highlight current topics in forest products research, including (1) industrial chemicals, biofuels, and energy from woody materials; (2) wood-basedmore » activated carbon and carbon nanostructures; (3) development of improved wood protection treatments; (4) massive timber construction; (5) wood as a bioinspiring material; and (6) atomic simulations of wood polymers. We conclude with a discussion of the sustainability of wood as a renewable forest resource.« less

  10. Not Just Lumber—Using Wood in the Sustainable Future of Materials, Chemicals, and Fuels

    NASA Astrophysics Data System (ADS)

    Jakes, Joseph E.; Arzola, Xavier; Bergman, Rick; Ciesielski, Peter; Hunt, Christopher G.; Rahbar, Nima; Tshabalala, Mandla; Wiedenhoeft, Alex C.; Zelinka, Samuel L.

    2016-09-01

    Forest-derived biomaterials can play an integral role in a sustainable and renewable future. Research across a range of disciplines is required to develop the knowledge necessary to overcome the challenges of incorporating more renewable forest resources in materials, chemicals, and fuels. We focus on wood specifically because in our view, better characterization of wood as a raw material and as a feedstock will lead to its increased utilization. We first give an overview of wood structure and chemical composition and then highlight current topics in forest products research, including (1) industrial chemicals, biofuels, and energy from woody materials; (2) wood-based activated carbon and carbon nanostructures; (3) development of improved wood protection treatments; (4) massive timber construction; (5) wood as a bioinspiring material; and (6) atomic simulations of wood polymers. We conclude with a discussion of the sustainability of wood as a renewable forest resource.

  11. Wood phenology: from organ-scale processes to terrestrial ecosystem models

    NASA Astrophysics Data System (ADS)

    Delpierre, Nicolas; Guillemot, Joannès

    2016-04-01

    In temperate and boreal trees, a dormancy period prevents organ development during adverse climatic conditions. Whereas the phenology of leaves and flowers has received considerable attention, to date, little is known regarding the phenology of other tree organs such as wood, fine roots, fruits and reserve compounds. In this presentation, we review both the role of environmental drivers in determining the phenology of wood and the models used to predict its phenology in temperate and boreal forest trees. Temperature is a key driver of the resumption of wood activity in spring. There is no such clear dominant environmental cue involved in the cessation of wood formation in autumn, but temperature and water stress appear as prominent factors. We show that wood phenology is a key driver of the interannual variability of wood growth in temperate tree species. Incorporating representations of wood phenology in a terrestrial ecosystem model substantially improved the simulation of wood growth under current climate.

  12. Insights on wood combustion generated proinflammatory ultrafine particles (UFP).

    PubMed

    Corsini, Emanuela; Ozgen, Senem; Papale, Angela; Galbiati, Valentina; Lonati, Giovanni; Fermo, Paola; Corbella, Lorenza; Valli, Gianluigi; Bernardoni, Vera; Dell'Acqua, Manuela; Becagli, Silvia; Caruso, Donatella; Vecchi, Roberta; Galli, Corrado L; Marinovich, Marina

    2017-01-15

    This study aimed to collect, characterize ultrafine particles (UFP) generated from the combustion of wood pellets and logs (softwood and hardwood) and to evaluate their pro-inflammatory effects in THP-1 and A549 cells. Both cell lines responded to UFP producing interleukin-8 (IL-8), with wood log UFP being more active compared to pellet UFP. With the exception of higher effect observed with beech wood log UFP in THP-1, the ability of soft or hard woods to induce IL-8 release was similar. In addition, on weight mass, IL-8 release was similar or lower compared to diesel exhaust particles (DEP), arguing against higher biological activity of smaller size particles. UFP-induced IL-8 could be reduced by SB203580, indicating a role of p38MAPK activation in IL-8 production. The higher activity of beech wood log UFP in THP-1 was not due to higher uptake or endotoxin contamination. Qualitatively different protein adsorption profiles were observed, with less proteins bound to beech UFP compared to conifer UFP or DEP, which may provide higher intracellular availability of bioactive components, i.e. levoglucosan and galactosan, toward which THP-1 were more responsive compared to A549 cells. These results contribute to our understanding of particles emitted by domestic appliances and their biological effects. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  13. Spatially resolved metabolic analysis reveals a central role for transcriptional control in carbon allocation to wood.

    PubMed

    Roach, Melissa; Arrivault, Stéphanie; Mahboubi, Amir; Krohn, Nicole; Sulpice, Ronan; Stitt, Mark; Niittylä, Totte

    2017-06-15

    The contribution of transcriptional and post-transcriptional regulation to modifying carbon allocation to developing wood of trees is not well defined. To clarify the role of transcriptional regulation, the enzyme activity patterns of eight central primary metabolism enzymes across phloem, cambium, and developing wood of aspen (Populus tremula L.) were compared with transcript levels obtained by RNA sequencing of sequential stem sections from the same trees. Enzymes were selected on the basis of their importance in sugar metabolism and in linking primary metabolism to lignin biosynthesis. Existing enzyme assays were adapted to allow measurements from ~1 mm3 sections of dissected stem tissue. These experiments provided high spatial resolution of enzyme activity changes across different stages of wood development, and identified the gene transcripts probably responsible for these changes. In most cases, there was a clear positive relationship between transcripts and enzyme activity. During secondary cell wall formation, the increases in transcript levels and enzyme activities also matched with increased levels of glucose, fructose, hexose phosphates, and UDP-glucose, emphasizing an important role for transcriptional regulation in carbon allocation to developing aspen wood. These observations corroborate the efforts to increase carbon allocation to wood by engineering gene regulatory networks. © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  14. Gamma scintigraphic study of the hydrodynamically balanced matrix tablets of Metformin HCl in rabbits

    PubMed Central

    Razavi, Mahboubeh; Karimian, Hamed; Yeong, Chai Hong; Sarji, Sazilah Ahmad; Chung, Lip Yong; Nyamathulla, Shaik; Noordin, Mohamed Ibrahim

    2015-01-01

    The purpose of this study is to evaluate the in vitro and in vivo performance of gastro-retentive matrix tablets having Metformin HCl as model drug and combination of natural polymers. A total of 16 formulations were prepared by a wet granulation method using xanthan, tamarind seed powder, tamarind kernel powder and salep as the gel-forming agents and sodium bicarbonate as a gas-forming agent. All the formulations were evaluated for compendial and non-compendial tests and in vitro study was carried out on a USP-II dissolution apparatus at a paddle speed of 50 rpm. MOX2 formulation, composed of salep and xanthan in the ratio of 4:1 with 96.9% release, was considered as the optimum formulation with more than 90% release in 12 hours and short floating lag time. In vivo study was carried out using gamma scintigraphy in New Zealand White rabbits, optimized formulation was incorporated with 10 mg of 153Sm for labeling MOX2 formulation. The radioactive samarium oxide was used as the marker to trace transit of the tablets in the gastrointestinal tract. The in vivo data also supported retention of MOX2 formulation in the gastric region for 12 hours and were different from the control formulation without a gas and gel forming agent. It was concluded that the prepared floating gastro-retentive matrix tablets had a sustained-release effect in vitro and in vivo, gamma scintigraphy played an important role in locating the oral transit and the drug-release pattern. PMID:26124637

  15. Effect of Tamarindus indica leaf powder on plasma concentrations of copper, zinc, and iron in fluorotic cows

    PubMed Central

    Samal, Pinaki; Patra, R. C.; Gupta, A. R.; Mishra, S. K.; Jena, D.; Satapathy, D.

    2016-01-01

    Aim: The main objective of the study was to determine the deleterious effect of fluoride on plasma trace minerals of fluorotic cattle and to evaluate the effect of Tamarindus indica leaf powder toward correction of the same. Materials and Methods: A total of 30 cattle exhibiting chronic sign of fluorosis and 10 healthy cattle from nonfluorotic area were incorporated in this study. Fluorotic cattle were divided into three equal groups consisting of 10 cattle each. Group I from fluoride free area served as healthy control. The Group II received no treatment and served as disease control. Groups III and IV were supplemented with tamarind leaf powder at 15 g and 30 g/day with feed for 60 days. Plasma mineral status was evaluated after 60 days of treatment with double beam atomic absorption spectrophotometer. Results: Statistical analysis of data revealed a significant (p<0.05) decrease in mean plasma copper (Cu) (0.344±0.007 ppm), zinc (Zn) (0.692±0.06 ppm), and iron (Fe) concentration (1.100±0.01 ppm) in fluorotic cattle in comparison to healthy cattle (0.58±0.010, 2.342±0.04, 1.406±0.04 ppm, respectively). Significant (p<0.05) increase in Cu, Zn, and Fe was recorded after supplementation of tamarind leaf powder to the fluorotic cattle. Conclusion: It was concluded that fluorotic cattle might be supplemented with T. indica leaf powder with feed for the correction of the decreased level of certain plasma minerals. PMID:27847422

  16. Efficacy of chicken litter and wood biochars and their activated counterparts in heavy metal clean up from wastewater

    USDA-ARS?s Scientific Manuscript database

    It is known that properties of activated biochars are tightly associated with those of the original feedstock as well as pyrolysis and activation conditions. This study examined two feedstock types, pine wood shavings and chicken litter, to produce biochars at two different pyrolysis temperatures a...

  17. Interactions between nesting pileated woodpeckers and wood ducks

    Treesearch

    Richard N. Conner; Clifford E. Shackelford; Daniel Saenz; Richard R. Schaefer

    2001-01-01

    We observed interactions between a nesting pair of pileated woodpeckers (Dryocopus pileatus) and what appeared to be four pairs of wood ducks (Aix sponsa). Wood ducks regularly approached and attempted to enter an active pileated woodpecker nest cavity that contained three fully feathered young pileated woodpeckers. The male...

  18. Dynamics of active layer in wooded palsas of northern Quebec

    NASA Astrophysics Data System (ADS)

    Jean, Mélanie; Payette, Serge

    2014-02-01

    Palsas are organic or mineral soil mounds having a permafrost core. Palsas are widespread in the circumpolar discontinuous permafrost zone. The annual dynamics and evolution of the active layer, which is the uppermost layer over the permafrost table and subjected to the annual freeze-thaw cycle, are influenced by organic layer thickness, snow depth, vegetation type, topography and exposure. This study examines the influence of vegetation types, with an emphasis on forest cover, on active layer dynamics of palsas in the Boniface River watershed (57°45‧ N, 76°00‧ W). In this area, palsas are often colonized by black spruce trees (Picea mariana (Mill.) B.S.P.). Thaw depth and active layer thickness were monitored on 11 wooded or non-wooded mineral and organic palsas in 2009, 2010 and 2011. Snow depth, organic layer thickness, and vegetation types were assessed. The mapping of a palsa covered by various vegetation types and a large range of organic layer thickness were used to identify the factors influencing the spatial patterns of thaw depth and active layer. The active layer was thinner and the thaw rate slower in wooded palsas, whereas it was the opposite in more exposed sites such as forest openings, shrubs and bare ground. Thicker organic layers were associated with thinner active layers and slower thaw rates. Snow depth was not an important factor influencing active layer dynamics. The topography of the mapped palsa was uneven, and the environmental factors such as organic layer, snow depth, and vegetation types were heterogeneously distributed. These factors explain a part of the spatial variation of the active layer. Over the 3-year long study, the area of one studied palsa decreased by 70%. In a context of widespread permafrost decay, increasing our understanding of factors that influence the dynamics of wooded and non-wooded palsas and understanding of the role of vegetation cover will help to define the response of discontinuous permafrost landforms to changing climatic conditions.

  19. Visible-light activate Ag/WO3 films based on wood with enhanced negative oxygen ions production properties

    NASA Astrophysics Data System (ADS)

    Gao, Likun; Gan, Wentao; Cao, Guoliang; Zhan, Xianxu; Qiang, Tiangang; Li, Jian

    2017-12-01

    The Ag/WO3-wood was fabricated through a hydrothermal method and a silver mirror reaction. The system of visible-light activate Ag/WO3-wood was used to produce negative oxygen ions, and the effect of Ag nanoparticles on negative oxygen ions production was investigated. From the results of negative oxygen ions production tests, it can be observed that the sample doped with Ag nanoparticles, the concentration of negative oxygen ions is up to 1660 ions/cm3 after 60 min visible light irradiation. Moreover, for the Ag/WO3-wood, even after 60 min without irradiation, the concentration of negative oxygen ions could keep more than 1000 ions/cm3, which is up to the standard of the fresh air. Moreover, due to the porous structure of wood, the wood acted as substrate could promote the nucleation of nanoparticles, prevent the agglomeration of the particles, and thus lead the improvement of photocatalytic properties. And such wood-based functional materials with the property of negative oxygen ions production could be one of the most promising materials in the application of indoor decoration materials, which would meet people's pursuit of healthy, environment-friendly life.

  20. 2006 : Wood Used in Residential Repair and Remodeling U.S. and Canada, with Comparison to 1997 and 2003 : Executive Summary

    Treesearch

    Craig Adair; David B. McKeever

    2009-01-01

    The repair and remodeling of residential units in both the United States and Canada is an important market for wood products. Many and varied repair and remodeling activities and projects are undertaken annually. Some require substantial amounts of wood products, some none at all. In 2006, about 28 percent of all solid wood products consumed in the United States and 31...

  1. Do black ducks and wood ducks habituate to aircraft disturbance?

    USGS Publications Warehouse

    Conomy, J.T.; Dubovsky, J.A.; Collazo, J.A.; Fleming, W.J.

    1998-01-01

    Requests to increase military aircraft activity in some training facilities in the United States have raised the need to determine if waterfowl and other wildlife are adversely affected by aircraft disturbance. We hypothesized that habituation was a possible proximate factor influencing the low proportion of free-ranging ducks reacting to military aircraft activities in a training range in coastal North Carolina during winters 1991 and 1992. To test this hypothesis, we subjected captive, wild-strain American black ducks (Anas rubripes) and wood ducks (Aix sponsa) to actual and simulated activities of jet aircraft. In the first experiment, we placed black ducks in an enclosure near the center of aircraft activities on Piney Island, a military aircraft target range in coastal North Carolina. The proportion of times black ducks reacted (e.g., alert posture, fleeing response) to visual and auditory aircraft activity decreased from 38 to 6% during the first 17 days of confinement. Response rates remained stable at 5.8% thereafter. In the second experiment, black ducks and wood ducks were exposed to 6 different recordings of jet noise. The proportion of times black ducks reacted to noise decreased (P 0.05) in time-activity budgets of black ducks between pre-exposure to noise and 24 hr after first exposure. Unlike black ducks, wood duck responses to jet noise did not decrease uniformly among experimental groups following initial exposure to noise (P = 0.01). We conclude that initial exposure to aircraft noise elicits behavioral responses from black ducks and wood ducks. With continued exposure of aircraft noise, black ducks may become habituated. However, wood ducks did not exhibit the same pattern of response, suggesting that the ability of waterfowl to habituate to aircraft noise may be species specific.

  2. Fast enhancement on hydrophobicity of poplar wood surface using low-pressure dielectric barrier discharges (DBD) plasma

    NASA Astrophysics Data System (ADS)

    Chen, Weimin; Zhou, Xiaoyan; Zhang, Xiaotao; Bian, Jie; Shi, Shukai; Nguyen, Thiphuong; Chen, Minzhi; Wan, Jinglin

    2017-06-01

    The hydrophilicity of woody products leads to deformation and cracks, which greatly limits its applications. Low-pressure dielectric barrier discharge (DBD) plasma using hexamethyldisiloxane was applied in poplar wood surface to enhance the hydrophobicity. The chemical properties, micro-morphology, and contact angles of poplar wood surface before and after plasma treatment were investigated by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), x-ray photoelectron spectroscopy (XPS), scanning electron microscope and energy dispersive analysis of X-ray (SEM-EDX), atomic force microscopy (AFM), and optical contact angle measurement (OCA). Moreover, tinfoil film was used as the base to reveal the enhancement mechanism. The results showed that hexamethyldisiloxane monomer is first broken into several fragments with active sites and hydrophobic chemical groups. Meanwhile, plasma treatment results in the formation of free radicals and active sites in the poplar wood surface. Then, the fragments are reacted with free radicals and incorporated into the active sites to form a network structure based on the linkages of Si-O-Si and Sisbnd Osbnd C. Plasma treatment also leads to the formation of acicular nano-structure in poplar wood surface. These facts synergistically enhance the hydrophobicity of poplar wood surface, demonstrating the dramatically increase in the equilibrium contact angle by 330%.

  3. [137Cs and 90Sr accumulation in birch wood (Betula pendula Roth.) growing in regions with different soil conditions].

    PubMed

    Perevolotskiĭ, A N; Bulavik, I M; Perevolotskaia, T V; Paskrobko, L A; Andrush, S N

    2005-01-01

    The studies of 137Cs and 90Sr accumulation characteristics by birch wood (Betula pendula Roth.) growing in different edaphic conditions. Were conducted was stated that the increase in soil fertility from trophotops A to D results in 5-fold 137Cs transfer factor decrease and 2-fold decrease of 90Sr. Soil humidity increase for each grade of trophic net results happens the increase of 137Cs transfer factors to wood and decrease for 90Sr. Total activity of 37Cs and 90Sr in birch wood plantation depends on plantation productivity and on radionuclide transfer factors depending on different plantation conditions. In the most prevailing edaphotops (B2, B3, C2 and C3) birch wood accumulates 0.6-1.2% of 137Cs and 13-19% of 90Sr from total activity of radionuclides in biogeocenosis.

  4. Uptake of ¹³⁷Cs by berries, mushrooms and needles of Scots pine in peatland forests after wood ash application.

    PubMed

    Vetikko, Virve; Rantavaara, Aino; Moilanen, Mikko

    2010-12-01

    Increasing use of wood fuels for energy production in Finland since the 1990s implies that large quantities of the generated ashes will be available for forest fertilization. The aim of this study was to analyse the effect of wood ash application on ¹³⁷Cs activity concentrations in Scots pine (Pinus sylvestris L.) needles and certain berries and mushrooms on drained peatlands. The study was based on field experiments carried out on two mires in Finland in 1997-1998. Two different types of wood ash were applied at dosages of 3500, 3700, 10 500 and 11 100 kg ha⁻¹. Wood ash did not increase ¹³⁷Cs activity concentration in plants in the second growing season following application. On the contrary, a decrease in ¹³⁷Cs activity concentration was seen in the plants of the ecosystem on drained peatlands. This result is of importance, for instance, when recycling of ash is being planned. Copyright © 2010 Elsevier Ltd. All rights reserved.

  5. The use of wood for wind turbine blade construction

    NASA Technical Reports Server (NTRS)

    Gougeon, M.; Zuteck, M.

    1979-01-01

    The interrelationships between moisture and wood, conditions for dry rot spore activity, the protection of wood fibers from moisture, wood resin composites, wood laminating, quality control, and the mechanical properties of wood are discussed. The laminated veneer and the bonded sawn stock fabrication techniques, used in the construction of a turbine blade with a monocoque 'D' section forming the leading edge and a built up trailing edge section, are described. A 20 foot root end sample complete with 24 bonded-in studs was successfully subjected to large onetime loads in both the flatwise and edgewise directions, and to fatigue tests. Results indicate that wood is both a viable and advantageous material for use in wind turbine blades. The basic material is reasonably priced, domestically available, ecologically sound, and easily fabricated with low energy consumption.

  6. Phytochemical screening and study of antioxidant, antimicrobial, antidiabetic, anti-inflammatory and analgesic activities of extracts from stem wood of Pterocarpus marsupium Roxburgh.

    PubMed

    Pant, Dipak Raj; Pant, Narayan Dutt; Saru, Dil Bahadur; Yadav, Uday Narayan; Khanal, Dharma Prasad

    2017-01-01

    The main aims of the study were to evaluate the phytochemical constituents and to study the antioxidant, antimicrobial, antidiabetic, anti-inflammatory, and analgesic activities of extracts from stem wood of Pterocarpus marsupium . Ethanol, acetone and isopropyl alcohol (IPA) (1:1) extracts of stem wood of P. marsupium were subjected to phytochemical screening and analysis of biological activities from August 2015 to January 2016. The antioxidant assay was carried out using 2, 2-diphenyl-1-picrylhydrazyl scavenging method, antimicrobial activity testing by cup diffusion method, antidiabetic test evaluation by oral glucose tolerance test in mice, anti-inflammatory effect was evaluated by hind paw edema method in mice and analgesic test evaluation by a chemical writhing method in mice. The results of the study revealed that P. marsupium is a source of various phytoconstituents such as alkaloids, glycosides, saponins, tannins, proteins, carbohydrates, cardiac glycosides, flavonoids, and terpenoids. Both the acetone and IPA extract as well as the ethanol extract of stem wood of P. marsupium exhibited a dose-dependent antioxidant activity. Acetone and IPA extract showed antibacterial activity against Gram-positive bacteria, while the ethanolic extract was found to possess antidiabetic activity. The antidiabetic activity of the extract was found to be time and dose-dependent. Similarly, the acetone and IPA extract was found to have anti-inflammatory activity, which was also time and dose-dependent. Furthermore, the ethanolic extract showed analgesic activity, which was dose-dependent. The ethanolic extract was found to be nontoxic. Thus, this study laid sufficient background for the further research on extracts from stem wood of P. marsupium for identification, subsequent purification and isolation of compounds having antibacterial, antidiabetic, anti-inflammatory, and analgesic activities.

  7. Saprophytic Activity and Sporulation of Cryphonectria parasitica on Dead Chestnut Wood in Forests with Naturally Established Hypovirulence.

    PubMed

    Prospero, S; Conedera, M; Heiniger, U; Rigling, D

    2006-12-01

    ABSTRACT Sustainable biological control of the chestnut blight fungus Crypho-nectria parasitica with hypovirulence depends on the production and dissemination of hypovirus-infected propagules of the pathogen. We investigated the ability of C. parasitica to sporulate and produce hypo-virus-infected spores on recently dead chestnut wood in coppice stands in southern Switzerland where hypovirulence has been naturally established. The number and type (active, inactive, or none) of cankers was assessed on experimentally cut and stacked stems, firewood stacks, and natural dead wood. Hypovirus-free and hypovirus-infected strains readily survived for more than 1 year in the chestnut blight cankers of the stacked stems. Sporulation of C. parasitica was observed on the surface of preexisting inactive and active cankers, as well as on newly colonized bark areas and was significantly more abundant than on comparable cankers on living stems. On all types of dead wood, we observed more stromata with perithecia than with pycnidia; however, a large proportion of the stromata was not differentiated. All perithecia examined yielded only hypovirus-free ascospores. The incidence of pycnidia that produced hypovirus-infected conidia ranged from 5% on natural dead wood to 41% on the experimental stacks. The mean virus transmission rate into conidia was 69%. Our study demonstrates a considerable saprophytic activity of C. parasitica on recently dead chestnut wood and supports the hypothesis of a role of this saprophytic phase in the epidemiology of hypovirulence.

  8. Pyranose Oxidase, a Major Source of H(2)O(2) during Wood Degradation by Phanerochaete chrysosporium, Trametes versicolor, and Oudemansiella mucida.

    PubMed

    Daniel, G; Volc, J; Kubatova, E

    1994-07-01

    The production of the H(2)O(2)-generating enzyme pyranose oxidase (POD) (EC 1.1.3.10) (synonym, glucose 2-oxidase), two ligninolytic peroxidases, and laccase in wood decayed by three white rot fungi was investigated by correlated biochemical, immunological, and transmission electron microscopic techniques. Enzyme activities were assayed in extracts from decayed birch wood blocks obtained by a novel extraction procedure. With the coupled peroxidase-chromogen (3-dimethylaminobenzoic acid plus 3-methyl-2-benzothiazolinone hydrazone hydrochloride) spectrophotometric assay, the highest POD activities were detected in wood blocks degraded for 4 months and were for Phanerochaete chrysosporium (149 mU g [dry weight] of decayed wood), Trametes versicolor (45 mU g), and Oudemansiella mucida (1.2 mU g), corresponding to wood dry weight losses of 74, 58, and 13%, respectively. Mn-dependent peroxidase activities in the same extracts were comparable to those of POD, while lignin peroxidase activity was below the detection limit for all fungi with the veratryl alcohol assay. Laccase activity was high with T. versicolor (422 mU g after 4 months), in trace levels with O. mucida, and undetectable in P. chrysosporium extracts. Evidence for C-2 specificity of POD was shown by thin-layer chromatography detection of 2-keto-d-glucose as the reaction product. By transmission electron microscopy-immunocytochemistry, POD was found to be preferentially localized in the hyphal periplasmic space of P. chrysosporium and O. mucida and associated with membranous materials in hyphae growing within the cell lumina or cell walls of partially and highly degraded birch fibers. An extracellular distribution of POD associated with slime coating wood cell walls was also noted. The periplasmic distribution in hyphae and extracellular location of POD are consistent with the reported ultrastructural distribution of H(2)O(2)-dependent Mn-dependent peroxidases. This fact and the dominant presence of POD and Mn-dependent peroxidase in extracts from degraded wood suggest a cooperative role of the two enzymes during white rot decay by the test fungi.

  9. Molecular control of wood formation in trees.

    PubMed

    Ye, Zheng-Hua; Zhong, Ruiqin

    2015-07-01

    Wood (also termed secondary xylem) is the most abundant biomass produced by plants, and is one of the most important sinks for atmospheric carbon dioxide. The development of wood begins with the differentiation of the lateral meristem, vascular cambium, into secondary xylem mother cells followed by cell expansion, secondary wall deposition, programmed cell death, and finally heartwood formation. Significant progress has been made in the past decade in uncovering the molecular players involved in various developmental stages of wood formation in tree species. Hormonal signalling has been shown to play critical roles in vascular cambium cell proliferation and a peptide-receptor-transcription factor regulatory mechanism similar to that controlling the activity of apical meristems is proposed to be involved in the maintenance of vascular cambium activity. It has been demonstrated that the differentiation of vascular cambium into xylem mother cells is regulated by plant hormones and HD-ZIP III transcription factors, and the coordinated activation of secondary wall biosynthesis genes during wood formation is mediated by a transcription network encompassing secondary wall NAC and MYB master switches and their downstream transcription factors. Most genes encoding the biosynthesis enzymes for wood components (cellulose, xylan, glucomannan, and lignin) have been identified in poplar and a number of them have been functionally characterized. With the availability of genome sequences of tree species from both gymnosperms and angiosperms, and the identification of a suite of wood-associated genes, it is expected that our understanding of the molecular control of wood formation in trees will be greatly accelerated. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  10. Cytochemical localization of cellulases in decayed and nondecayed wood

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Murmanis, L.; Highley, T.L.; Palmer, J.G.

    1987-01-01

    Sawdust from undecayed western hemlock wood and from wood previously decayed by the brown-rot fungus Poria placenta or by the white-rot fungus Ganoderma applanatum was incubated with commercial cellulase from Trichoderma viride. Samples were treated cytochemically to locate cellulase activity and examined by TEM. Results showed that cellulase degraded undecayed wood extensively, with the attack starting on the outer border of a cell wall and progressing inside. Wood decayed by P. placenta, with or without cellulase incubation, and treated by the cytochemical test showed uniform distribution of electron dense particles throughout the cell walls. In wood decayed by G. applanatum,more » cellulase degradation was similar to that in undecayed wood. From measurements of particle diameter it is suggested that electron dense particles are cellulase. It is concluded that brown-rot and white-rot fungi have different effects on the microstructure of wood. The brown-rot fungus appears to open the wood microstructure so that cellulase can diffuse throughout the degraded tracheid wall.« less

  11. Antioxidant effects of four heartwood extractives on midgut enzyme activity in Heterotermes indicola (Blattodea: Rhinotermitidae)

    Treesearch

    Babar Hassan; Sohail Ahmed; Grant Kirker; Mark E Mankowski; Muhammad Misbah-ul-Haq

    2018-01-01

    Heterotermes indicola (Wasmann) (Blattodea: Rhinotermitidae) is a species of subterranean termite that is a destructive pest of wood and wood products in Pakistan. This study evaluated the antioxidant and antienzyme potential of heartwood extractives against H. indicola. Heartwood extractives of four durable wood species, Tectona grandis...

  12. Scoring a Goal for Learning

    ERIC Educational Resources Information Center

    Graham, Renee Williams; Dennis, Emily; Cornell, Marilyn

    2013-01-01

    Students in Mrs. Cornell's kindergarten class shared their observations about what happened when drops of water were placed on different types of wood. The students were engaging in a science lesson focusing on the observable properties of wood, an activity from the FOSS Wood and Paper science module. This lesson is one of many that Mrs. Cornell…

  13. Effects of nutrient enrichment on the decomposition of wood and associated microbial activity in streams

    USGS Publications Warehouse

    Gulis, V.; Rosemond, A.D.; Suberkropp, K.; Weyers, H.S.; Benstead, J.P.

    2004-01-01

    1. We determined the effects of nutrient enrichment on wood decomposition rates and microbial activity during a 3-year study in two headwater streams at Coweeta Hydrologic Laboratory, NC, U.S.A. After a 1-year pretreatment period, one of the streams was continuously enriched with inorganic nutrients (nitrogen and phosphorus) for 2 years while the other stream served as a reference. We determined the effects of enrichment on both wood veneers and sticks, which have similar carbon quality but differ in physical characteristics (e.g. surface area to volume ratios, presence of bark) that potentially affect microbial colonisation and activity. 2. Oak wood veneers (0.5 mm thick) were placed in streams monthly and allowed to decompose for approximately 90 days. Nutrient addition stimulated ash-free dry mass loss and increased mean nitrogen content, fungal biomass and microbial respiration on veneers in the treatment stream compared with the reference. The magnitude of the response to enrichment was great, with mass loss 6.1 times, and per cent N, fungal biomass and microbial respiration approximately four times greater in the treatment versus reference stream. 3. Decomposition rate and nitrogen content of maple sticks (ca. 1-2 cm diameter) also increased; however, the effect was less pronounced than for veneers. Wood response overall was greater than that determined for leaves in a comparable study, supporting the hypothesis that response to enrichment may be greater for lower quality organic matter (high C:N) than for higher quality (low C:N) substrates. 4. Our results show that moderate nutrient enrichment can profoundly affect decomposition rate and microbial activity on wood in streams. Thus, the timing and availability of wood that provides retention, structure, attachment sites and food in stream ecosystems may be affected by nutrient concentrations raised by human activities.

  14. Dioxin effects on wood duck (Aix sponsa) embryos from sites near paper mills

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Beeman, D.K.; Melancon, M.J.; Fleming, W.J.

    Biological and biochemical variables were studied in wood duck embryos from four dioxin-contaminated sites near paper mills in the Southeastern United States and three reference sites. Sites were selected based on a history of dioxin contamination in both sediments and fish. In addition, wood duck embryos collected downstream from an Arkansas Superfund site with demonstrated dioxin-induced reproductive impairment served as positive controls. Whole clutches of eggs were collected from the wild after fifteen days of incubation and mechanically incubated. Two embryos per clutch were sacrificed at pipping and liver monooxygenase activities (BROD, EROD and MROD) were quantified. Hatching success wasmore » determined for the remainder of the nest. Preliminary results indicate no difference in monooxygenase activities across sites even though the authors have previously demonstrated induction of monooxygenase activity in wood duck embryos in laboratory studies. In addition, there were no differences in weight at pipping, liver weight and liver weight to body weight ratios. No differences were seen in hatching success or weight at hatch nor were there any gross morphological abnormalities. This may indicate that exposure of wood ducks nesting near these pulp paper mills is below those which cause elevated monooxygenase activities and reproductive impairment.« less

  15. 3-Prenyl luteolin, a new prenylated flavone with melanin biosynthesis inhibitory activity from wood of Artocarpus heterophyllus.

    PubMed

    Arung, Enos Tangke; Shimizu, Kuniyoshi; Tanaka, Hiroyuki; Kondo, Ryuichiro

    2010-09-01

    In our efforts to find new whitening agent from natural resources, we focused on wood of Artocarpus heterophyllus which shows anti-melanogenesis activity. By activity-guided fractionation of A. heterophyllus wood extract, a new prenylated flavonoid, 3-prenyl luteolin (1) was isolated. The IC(50) of mushroom tyrosinase inhibitory activity of 1 was 76.3 microM. The results of the comparison with that of luteolin showed the prenyl substituent at C-3 position of 1 play an important role for revealing tyrosinase inhibition. In melanin formation inhibition on B16 melanoma cells, IC(50) of 1 was 56.7 microM with less cytotoxicity. Copyright (c) 2010 Elsevier B.V. All rights reserved.

  16. Interactions between soil- and dead wood-inhabiting fungal communities during the decay of Norway spruce logs

    PubMed Central

    Mäkipää, Raisa; Rajala, Tiina; Schigel, Dmitry; Rinne, Katja T; Pennanen, Taina; Abrego, Nerea; Ovaskainen, Otso

    2017-01-01

    We investigated the interaction between fungal communities of soil and dead wood substrates. For this, we applied molecular species identification and stable isotope tracking to both soil and decaying wood in an unmanaged boreal Norway spruce-dominated stand. Altogether, we recorded 1990 operational taxonomic units, out of which more than 600 were shared by both substrates and 589 were found to exclusively inhabit wood. On average the soil was more species-rich than the decaying wood, but the species richness in dead wood increased monotonically along the decay gradient, reaching the same species richness and community composition as soil in the late stages. Decaying logs at all decay stages locally influenced the fungal communities from soil, some fungal species occurring in soil only under decaying wood. Stable isotope analyses suggest that mycorrhizal species colonising dead wood in the late decay stages actively transfer nitrogen and carbon between soil and host plants. Most importantly, Piloderma sphaerosporum and Tylospora sp. mycorrhizal species were highly abundant in decayed wood. Soil- and wood-inhabiting fungal communities interact at all decay phases of wood that has important implications in fungal community dynamics and thus nutrient transportation. PMID:28430188

  17. Interactions between soil- and dead wood-inhabiting fungal communities during the decay of Norway spruce logs.

    PubMed

    Mäkipää, Raisa; Rajala, Tiina; Schigel, Dmitry; Rinne, Katja T; Pennanen, Taina; Abrego, Nerea; Ovaskainen, Otso

    2017-09-01

    We investigated the interaction between fungal communities of soil and dead wood substrates. For this, we applied molecular species identification and stable isotope tracking to both soil and decaying wood in an unmanaged boreal Norway spruce-dominated stand. Altogether, we recorded 1990 operational taxonomic units, out of which more than 600 were shared by both substrates and 589 were found to exclusively inhabit wood. On average the soil was more species-rich than the decaying wood, but the species richness in dead wood increased monotonically along the decay gradient, reaching the same species richness and community composition as soil in the late stages. Decaying logs at all decay stages locally influenced the fungal communities from soil, some fungal species occurring in soil only under decaying wood. Stable isotope analyses suggest that mycorrhizal species colonising dead wood in the late decay stages actively transfer nitrogen and carbon between soil and host plants. Most importantly, Piloderma sphaerosporum and Tylospora sp. mycorrhizal species were highly abundant in decayed wood. Soil- and wood-inhabiting fungal communities interact at all decay phases of wood that has important implications in fungal community dynamics and thus nutrient transportation.

  18. Activity of two strobilurin fungicides against three species of decay fungi in agar plate tests

    Treesearch

    Juliet D. Tang; Tina Ciaramitaro; Maria Tomaso-Peterson; Susan V. Diehl

    2017-01-01

    The objective of this study was to examine the toxicity of strobilurin fungicides against wood decay fungi in order to assess their potential to act as a co-biocide for copper-based wood protection. Two strobilurin fungicides, Heritage (50% azoxystrobin active ingredient) and Insignia (20% pyraclostrobin active ingredients), and copper sulfate pentahydrate were tested...

  19. Protection of Historical Wood against Microbial Degradation-Selection and Application of Microbiocides.

    PubMed

    Koziróg, Anna; Rajkowska, Katarzyna; Otlewska, Anna; Piotrowska, Małgorzata; Kunicka-Styczyńska, Alina; Brycki, Bogumił; Nowicka-Krawczyk, Paulina; Kościelniak, Marta; Gutarowska, Beata

    2016-08-22

    The aim of this study was to select effective and safe microbiocides for the disinfection and protection of historical wooden surfaces at the former Auschwitz II-Birkenau concentration and extermination camp. We tested seven active compounds against bacteria and moulds, of which didecyldimethylammonium chloride and N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine were effective even at 0.02%-2%. Subsequently, eight microbiocides containing the selected active ingredients were chosen and applied three times on the surface of wood samples colonized by bacteria and moulds. ABM-1 and ABM-2-6% solution; Rocima 101-8%; Preventol R 80-12%; Acticide 706 LV-15% and Boramon-30% were the most effective disinfectants. Under laboratory conditions, ABM-1, Boramon and Rocima 101 ensured antimicrobial protection of new wood samples for six months. In situ, 30% Boramon and 8% Rocima 101 applied by spraying effectively protected the historical wood from bacterial and mould growth for 12 and 3 months, respectively. Colour and luminance of the new wood were not altered after exposure to the biocides. Boramon and Rocima 101, applied by the spraying method, caused no significant change in the colour of the historical wood. Results from this study were used to develop a procedure for the protection of wood in historical buildings against biodeterioration.

  20. Protection of Historical Wood against Microbial Degradation—Selection and Application of Microbiocides

    PubMed Central

    Koziróg, Anna; Rajkowska, Katarzyna; Otlewska, Anna; Piotrowska, Małgorzata; Kunicka-Styczyńska, Alina; Brycki, Bogumił; Nowicka-Krawczyk, Paulina; Kościelniak, Marta; Gutarowska, Beata

    2016-01-01

    The aim of this study was to select effective and safe microbiocides for the disinfection and protection of historical wooden surfaces at the former Auschwitz II-Birkenau concentration and extermination camp. We tested seven active compounds against bacteria and moulds, of which didecyldimethylammonium chloride and N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine were effective even at 0.02%–2%. Subsequently, eight microbiocides containing the selected active ingredients were chosen and applied three times on the surface of wood samples colonized by bacteria and moulds. ABM-1 and ABM-2—6% solution; Rocima 101—8%; Preventol R 80—12%; Acticide 706 LV—15% and Boramon—30% were the most effective disinfectants. Under laboratory conditions, ABM-1, Boramon and Rocima 101 ensured antimicrobial protection of new wood samples for six months. In situ, 30% Boramon and 8% Rocima 101 applied by spraying effectively protected the historical wood from bacterial and mould growth for 12 and 3 months, respectively. Colour and luminance of the new wood were not altered after exposure to the biocides. Boramon and Rocima 101, applied by the spraying method, caused no significant change in the colour of the historical wood. Results from this study were used to develop a procedure for the protection of wood in historical buildings against biodeterioration. PMID:27556450

  1. Shell-armored wood cobbles as a potential criterion for detrital coal deposits

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    DiMarco, M.J.; Nummedal, D.

    1986-01-01

    Shell-armored wood cobbles occur on detrital-peat beaches along the seaward edge of the Mississippi Delta. Shell material consists exclusively of Mulinia lateralis, a dwarf surf clam. Soft, heavy, waterlogged wood fragments are abraded and become armored by hard shells in response to wave activity on the beach. Although their preservation potential is suspect, fossilized shell-armored wood clasts would probably be recognized as a type of coal ball and might indicate an allochthonous origin for the host coal.

  2. Enrichment with Wood Blocks Does Not Affect Toxicity Assessment in an Exploratory Toxicology Model Using Sprague–Dawley Rats

    PubMed Central

    Ditewig, Amy C; Bratcher, Natalie A; Davila, Donna R; Dayton, Brian D; Ebert, Paige; Lesuisse, Philippe; Liguori, Michael J; Wetter, Jill M; Yang, Hyuna; Buck, Wayne R

    2014-01-01

    Environmental enrichment in rodents may improve animal well-being but can affect neurologic development, immune system function, and aging. We tested the hypothesis that wood block enrichment affects the interpretation of traditional and transcriptomic endpoints in an exploratory toxicology testing model using a well-characterized reference compound, cyclophosphamide. ANOVA was performed to distinguish effects of wood block enrichment separate from effects of 40 mg/kg cyclophosphamide treatment. Biologically relevant and statistically significant effects of wood block enrichment occurred only for body weight gain. ANOVA demonstrated the expected effects of cyclophosphamide on food consumption, spleen weight, and hematology. According to transcriptomic endpoints, cyclophosphamide induced fewer changes in gene expression in liver than in spleen. Splenic transcriptomic pathways affected by cyclophosphamide included: iron hemostasis; vascular tissue angiotensin system; hepatic stellate cell activation and fibrosis; complement activation; TGFβ-induced hypertrophy and fibrosis; monocytes, macrophages, and atherosclerosis; and platelet activation. Changes in these pathways due to cyclophosphamide treatment were consistent with bone marrow toxicity regardless of enrichment. In a second study, neither enrichment nor type of cage flooring altered body weight or food consumption over a 28-d period after the first week. In conclusion, wood block enrichment did not interfere with a typical exploratory toxicology study; the effects of ingested wood on drug level kinetics may require further consideration. PMID:24827566

  3. Activation of Aspen Wood with Carbon Dioxide and Phosphoric Acid for Removal of Total Organic Carbon from Oil Sands Produced Water: Increasing the Yield with Bio-Oil Recycling

    PubMed Central

    Veksha, Andrei; Bhuiyan, Tazul I.; Hill, Josephine M.

    2016-01-01

    Several samples of activated carbon were prepared by physical (CO2) and chemical (H3PO4) activation of aspen wood and tested for the adsorption of organic compounds from water generated during the recovery of bitumen using steam assisted gravity drainage. Total organic carbon removal by the carbon samples increased proportionally with total pore volume as determined from N2 adsorption isotherms at −196 °C. The activated carbon produced by CO2 activation had similar removal levels for total organic carbon from the water (up to 70%) to those samples activated with H3PO4, but lower yields, due to losses during pyrolysis and activation. A method to increase the yield when using CO2 activation was proposed and consisted of recycling bio-oil produced from previous runs to the aspen wood feed, followed by either KOH addition (0.48%) or air pretreatment (220 °C for 3 h) before pyrolysis and activation. By recycling the bio-oil, the yield of CO2 activated carbon (after air pretreatment of the mixture) was increased by a factor of 1.3. Due to the higher carbon yield, the corresponding total organic carbon removal, per mass of wood feed, increased by a factor of 1.2 thus improving the overall process efficiency. PMID:28787817

  4. Living on the Edge: Contrasted Wood-Formation Dynamics in Fagus sylvatica and Pinus sylvestris under Mediterranean Conditions

    PubMed Central

    Martinez del Castillo, Edurne; Longares, Luis A.; Gričar, Jožica; Prislan, Peter; Gil-Pelegrín, Eustaquio; Čufar, Katarina; de Luis, Martin

    2016-01-01

    Wood formation in European beech (Fagus sylvatica L.) and Scots pine (Pinus sylvestris L.) was intra-annually monitored to examine plastic responses of the xylem phenology according to altitude in one of the southernmost areas of their distribution range, i.e., in the Moncayo Natural Park, Spain. The monitoring was done from 2011 to 2013 at 1180 and 1580 m a.s.l., corresponding to the lower and upper limits of European beech forest in this region. Microcores containing phloem, cambium and xylem were collected biweekly from twenty-four trees from the beginning of March to the end of November to assess the different phases of wood formation. The samples were prepared for light microscopy to observe the following phenological phases: onset and end of cell production, onset and end of secondary wall formation in xylem cells and onset of cell maturation. The temporal dynamics of wood formation widely differed among years, altitudes and tree species. For Fagus sylvatica, the onset of cambial activity varied between the first week of May and the third week of June. Cambial activity then slowed down and stopped in summer, resulting in a length of growing season of 48–75 days. In contrast, the growing season for P. sylvestris started earlier and cambium remained active in autumn, leading to a period of activity varying from 139-170 days. The intra-annual wood-formation pattern is site and species-specific. Comparison with other studies shows a clear latitudinal trend in the duration of wood formation, positive for Fagus sylvatica and negative for P. sylvestris. PMID:27047534

  5. Living on the Edge: Contrasted Wood-Formation Dynamics in Fagus sylvatica and Pinus sylvestris under Mediterranean Conditions.

    PubMed

    Martinez Del Castillo, Edurne; Longares, Luis A; Gričar, Jožica; Prislan, Peter; Gil-Pelegrín, Eustaquio; Čufar, Katarina; de Luis, Martin

    2016-01-01

    Wood formation in European beech (Fagus sylvatica L.) and Scots pine (Pinus sylvestris L.) was intra-annually monitored to examine plastic responses of the xylem phenology according to altitude in one of the southernmost areas of their distribution range, i.e., in the Moncayo Natural Park, Spain. The monitoring was done from 2011 to 2013 at 1180 and 1580 m a.s.l., corresponding to the lower and upper limits of European beech forest in this region. Microcores containing phloem, cambium and xylem were collected biweekly from twenty-four trees from the beginning of March to the end of November to assess the different phases of wood formation. The samples were prepared for light microscopy to observe the following phenological phases: onset and end of cell production, onset and end of secondary wall formation in xylem cells and onset of cell maturation. The temporal dynamics of wood formation widely differed among years, altitudes and tree species. For Fagus sylvatica, the onset of cambial activity varied between the first week of May and the third week of June. Cambial activity then slowed down and stopped in summer, resulting in a length of growing season of 48-75 days. In contrast, the growing season for P. sylvestris started earlier and cambium remained active in autumn, leading to a period of activity varying from 139-170 days. The intra-annual wood-formation pattern is site and species-specific. Comparison with other studies shows a clear latitudinal trend in the duration of wood formation, positive for Fagus sylvatica and negative for P. sylvestris.

  6. Vertical feed stick wood fuel burning furnace system

    DOEpatents

    Hill, Richard C.

    1984-01-01

    A new and improved stove or furnace for efficient combustion of wood fuel including a vertical feed combustion chamber for receiving and supporting wood fuel in a vertical attitude or stack, a major upper portion of the combustion chamber column comprising a water jacket for coupling to a source of water or heat transfer fluid and for convection circulation of the fluid for confining the locus of wood fuel combustion to the bottom of the vertical gravity feed combustion chamber. A flue gas propagation delay channel extending from the laterally directed draft outlet affords delayed travel time in a high temperature environment to assure substantially complete combustion of the gaseous products of wood burning with forced air as an actively induced draft draws the fuel gas and air mixture laterally through the combustion and high temperature zone. Active sources of forced air and induced draft are included, multiple use and circuit couplings for the recovered heat, and construction features in the refractory material substructure and metal component superstructure.

  7. [Bacterium Arthrobacter agilis UMCV2 and diverse amines inhibit in vitro growth of wood-decay fungi].

    PubMed

    Orozco-Mosqueda, M Del Carmen; Valencia-Cantero, Eduardo; López-Albarrán, Pablo; Martínez-Pacheco, Mauro; Velázquez-Becerra, Crisanto

    2015-01-01

    The kingdom Fungi is represented by a large number of organisms, including pathogens that deteriorate the main structural components of wood, such as cellulose, hemicellulose and lignin. The aim of our work was to characterize the antifungal activity in Arthrobacter agilis UMCV2 and diverse amines against wood-decaying fungi. Four fungal organisms (designated as UMTM) were isolated from decaying wood samples obtained from a forest in Cuanajo-Michoacán, México. Two of them showed a clear enzymatic activity of cellulases, xylanases and oxido-reducing enzymes and were identified as Hypocrea (UMTM3 isolate) and Fusarium (UMTM13 isolate). In vitro, the amines showed inhibitory effect against UMTM growth and one of the amines, dimethylhexadecylamine (DMA16), exhibited strong potential as wood preventive treatment, against the attack of decaying fungi. Copyright © 2015 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

  8. Estimated Hardwood Volume Available for Wood Chipmills or Other Low Grade Uses

    EPA Pesticide Factsheets

    The potential of wood chip mills to influence the distribution of harvests and the dynamics of wood fiber utilization has become an issue of concern. Where wood chip mills are active in a timber market, they may increase the utilization of wood fiber derived from a given harvest; change the pattern and distribution of harvests on the landscape; and reduce production costs of fiber supply, thus increasing overall quantity supplied in a price-taking market. Wood chip mills have proliferated in the South in recent decades. This data layer was created to address the following question: If the Mid-Atlantic region behaves as the South, which areas are relatively more attractive as sources of low grade hardwood fiber, and therefore relatively more vulnerable to any negative stresses associated with wood chip harvests? The data layer simulates annual softwood wood chip volume (in tons) available to supply chip mills or other low grade uses, if timber supply in the Mid-Atlantic mirrors trends for the South.

  9. Wood mimetic hydrogel beads for enzyme immobilization.

    PubMed

    Park, Saerom; Kim, Sung Hee; Won, Keehoon; Choi, Joon Weon; Kim, Yong Hwan; Kim, Hyung Joo; Yang, Yung-Hun; Lee, Sang Hyun

    2015-01-22

    Wood component-based composite hydrogels have potential applications in biomedical fields owing to their low cost, biodegradability, and biocompatibility. The controllable properties of wood mimetic composites containing three major wood components are useful for enzyme immobilization. Here, lipase from Candida rugosa was entrapped in wood mimetic beads containing cellulose, xylan, and lignin by dissolving wood components with lipase in [Emim][Ac], followed by reconstitution. Lipase entrapped in cellulose/xylan/lignin beads in a 5:3:2 ratio showed the highest activity; this ratio is very similar to that in natural wood. The lipase entrapped in various wood mimetic beads showed increased thermal and pH stability. The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel were 31- and 82-times higher than those of free lipase during incubation under denaturing conditions of high temperature and low pH, respectively. Owing to their biocompatibility, biodegradability, and controllable properties, wood mimetic hydrogel beads can be used to immobilize various enzymes for applications in the biomedical, bioelectronic, and biocatalytic fields. Copyright © 2014 Elsevier Ltd. All rights reserved.

  10. Corrosion of Embedded Metals in Wood: An Overview of Recent Research with Implications for building moisture design

    Treesearch

    Samuel L. Zelinka

    2013-01-01

    ASHRAE Standard 160, Criteria for Moisture-Control Design Analysis in Buildings, specifies moisture design criteria in buildings to prevent moisture damage such as fungal activity and corrosion. While there has been much research on mold and decay fungi in wood buildings, it is often overlooked that wet wood is corrosive to the metal screws...

  11. Exploring the hypothesis that limiting diffusion of fungal oxidants underlies decay resistance in acetylated wood

    Treesearch

    Christopher G. Hunt; Steven Lacher; Kolby Hirth; Linda Lorenz; Kenneth E. Hammel

    2017-01-01

    The mechanisms by which chemical modifications, specifically acetylation, improve the decay resistance of wood are a topic of active research. In the early stages of decay, fungi secrete lowmolecular- weight oxidants or oxidant precursors. These oxidants diffuse through the wet wood cell wall and oxidize cell wall polymers, which enable the decay process to proceed....

  12. National Trade can Drive Range Expansion of Bark- and Wood-Boring Beetles

    Treesearch

    Davide Rassati; Robert A Haack; Miloš Knížek; Massimo Faccoli

    2017-01-01

    Several native species of bark- and wood-boring beetles (Coleoptera) have expanded their range within their native biogeographic regions in the last years, but the role of human activity in driving this phenomenon has been underinvestigated. Here we analyze 3 yr of trapping records of native bark- and wood-boring beetles (Cerambycidae and Scolytinae) collected at 12...

  13. FORDAT : an information retrieval system for forest economic data

    Treesearch

    Henry M. Spelter

    1981-01-01

    Time series data frequently used in Forest Service studies of wood products consumption have been stored in a data retrieval system on the computer of the University of Wisconsin. The data cover activity in wood processing from forest to end use. Prices and costs at succeeding stages, historical usage, production rates, and other relevant data to wood use analysis were...

  14. Integrated control of wood destroying basidiomycetes combining Cu-based wood preservatives and Trichoderma spp.

    PubMed Central

    2017-01-01

    The production of new generation of wood preservatives (without addition of a co-biocide) in combination with an exchange of wood poles on identical sites with high fungal inoculum, has resulted in an increase of premature failures of wood utility poles in the last decades. Wood destroying basidiomycetes inhabiting sites where poles have been installed, have developed resistance against wood preservatives. The objective of the in vitro studies was to identify a Trichoderma spp. with a highly antagonistic potential against wood destroying basidiomycetes that is capable of colonizing Cu-rich environments. For this purpose, the activity of five Trichoderma spp. on Cu-rich medium was evaluated according to its growth and sporulation rates. The influence of the selected Trichoderma spp. on wood colonization and degradation by five wood destroying basidiomycetes was quantitatively analyzed by means of dry weight loss of wood specimens. Furthermore, the preventative effect of the selected Trichoderma spp. in combination with four Cu-based preservatives was also examined by mass loss and histological changes in the wood specimens. Trichoderma harzianum (T-720) was considered the biocontrol agent with higher antagonistic potential to colonize Cu-rich environments (up to 0.1% CuSO4 amended medium). T. harzianum demonstrated significant preventative effect on wood specimens against four wood destroying basidiomycetes. The combined effect of T. harzianum and Cu-based wood preservatives demonstrated that after 9 months incubation with two wood destroying basidiomycetes, wood specimens treated with 3.8 kg m-3 copper-chromium had weight losses between 55–65%, whereas containers previously treated with T. harzianum had significantly lower weight losses (0–25%). Histological studies on one of the wood destroying basidiomycetes revealed typical decomposition of wood cells by brown-rot fungi in Cu-impregnated samples, that were notably absent in wood specimens previously exposed to T. harzianum. It is concluded that carefully selected Trichoderma isolates can be used for integrated wood protection against a range of wood destroying basidiomycetes and may have potential for integrated wood protection in the field. PMID:28379978

  15. Integrated control of wood destroying basidiomycetes combining Cu-based wood preservatives and Trichoderma spp.

    PubMed

    Ribera, Javier; Fink, Siegfried; Bas, Maria Del Carmen; Schwarze, Francis W M R

    2017-01-01

    The production of new generation of wood preservatives (without addition of a co-biocide) in combination with an exchange of wood poles on identical sites with high fungal inoculum, has resulted in an increase of premature failures of wood utility poles in the last decades. Wood destroying basidiomycetes inhabiting sites where poles have been installed, have developed resistance against wood preservatives. The objective of the in vitro studies was to identify a Trichoderma spp. with a highly antagonistic potential against wood destroying basidiomycetes that is capable of colonizing Cu-rich environments. For this purpose, the activity of five Trichoderma spp. on Cu-rich medium was evaluated according to its growth and sporulation rates. The influence of the selected Trichoderma spp. on wood colonization and degradation by five wood destroying basidiomycetes was quantitatively analyzed by means of dry weight loss of wood specimens. Furthermore, the preventative effect of the selected Trichoderma spp. in combination with four Cu-based preservatives was also examined by mass loss and histological changes in the wood specimens. Trichoderma harzianum (T-720) was considered the biocontrol agent with higher antagonistic potential to colonize Cu-rich environments (up to 0.1% CuSO4 amended medium). T. harzianum demonstrated significant preventative effect on wood specimens against four wood destroying basidiomycetes. The combined effect of T. harzianum and Cu-based wood preservatives demonstrated that after 9 months incubation with two wood destroying basidiomycetes, wood specimens treated with 3.8 kg m-3 copper-chromium had weight losses between 55-65%, whereas containers previously treated with T. harzianum had significantly lower weight losses (0-25%). Histological studies on one of the wood destroying basidiomycetes revealed typical decomposition of wood cells by brown-rot fungi in Cu-impregnated samples, that were notably absent in wood specimens previously exposed to T. harzianum. It is concluded that carefully selected Trichoderma isolates can be used for integrated wood protection against a range of wood destroying basidiomycetes and may have potential for integrated wood protection in the field.

  16. Evidence for biological shaping of hair ice

    NASA Astrophysics Data System (ADS)

    Hofmann, D.; Preuss, G.; Mätzler, C.

    2015-07-01

    An unusual ice type, called hair ice, grows on the surface of dead wood of broad-leaf trees at temperatures slightly below 0 °C. We describe this phenomenon and present physical, chemical, and biological investigations to gain insight in the properties and processes related to hair ice. Tests revealed that the biological activity of a winter-active fungus is required in the wood for enabling the growth of hair ice. We confirmed the fungus hypothesis originally suggested by Wegener (1918) by reproducing hair ice on wood samples. Treatment by heat and fungicide suppresses the formation of hair ice. Fruiting bodies of Asco- and Basidiomycota are identified on hair-ice-carrying wood. One species, Exidiopsis effusa (Ee), was present on all investigated samples. Both hair-ice-producing wood samples and those with killed fungus show essentially the same temperature variation, indicating that the heat produced by fungal metabolism is very small, that the freezing rate is not influenced by the fungus activity, and that ice segregation is the common mechanism of ice growth on the wood surface. The fungus plays the role of shaping the ice hairs and preventing them from recrystallisation. Melted hair ice indicates the presence of organic matter. Chemical analyses show a complex mixture of several thousand CHO(N,S) compounds similar to fulvic acids in dissolved organic matter (DOM). The evaluation reveals decomposed lignin as being the main constituent. Further work is needed to clarify its role in hair-ice growth and to identify the recrystallisation inhibitor.

  17. Evidence for biological shaping of hair ice

    NASA Astrophysics Data System (ADS)

    Hofmann, D.; Preuss, G.; Mätzler, C.

    2015-04-01

    An unusual ice type, called hair ice, grows on the surface of dead wood of broad-leaf trees at temperatures slightly below 0 °C. We describe this phenomenon and present physical, chemical, and biological investigations to gain insight in the properties and processes related to hair ice. Tests revealed that the biological activity of a winter-active fungus is required in the wood for enabling the growth of hair ice. We confirmed the fungus hypothesis originally suggested by Wegener (1918) by reproducing hair ice on wood samples. Treatment by heat and fungicide, respectively, suppresses the formation of hair ice. Fruiting bodies of Asco- and Basidiomycota are identified on hair-ice carrying wood. One species, Exidiopsis effusa (Ee), has been present on all investigated samples. Both hair-ice producing wood samples and those with killed fungus show essentially the same temperature variation, indicating that the heat produced by fungal metabolism is very small, that the freezing rate is not influenced by the fungus activity and that ice segregation is the common mechanism of ice growth at the wood surface. The fungus plays the role of shaping the ice hairs and to prevent them from recrystallisation. Melted hair ice indicates the presence of organic matter. Chemical analyses show a complex mixture of several thousand CHO(N,S)-compounds similar to fulvic acids in dissolved organic matter (DOM). The evaluation reveals decomposed lignin as the main constituent. Further work is needed to clarify its role in hair-ice growth and to identify the recrystallisation inhibitor.

  18. Rooted in Time. Weymouth Woods-Sandhills Nature Preserve: An Environmental Education Designed for Grades 5-6.

    ERIC Educational Resources Information Center

    North Carolina State Dept. of Environment, Health, and Natural Resources, Raleigh. Div. of Parks and Recreation.

    This curriculum guide was developed to provide environmental education through a series of hands-on activities for the classroom and the outdoor setting of Weymouth Woods-Sandhills Nature Preserve, North Carolina. This activity packet, designed for grades 5 and 6, meets established curriculum objectives of the North Carolina Department of Public…

  19. Effect of Seasonal Changes in Soil Temperature and Moisture on Wood Consumption and Foraging Activity of the Formosan Subterranean Termite (Isoptera: Rhinotermitidae)

    USDA-ARS?s Scientific Manuscript database

    The objective of this study was to determine whether seasonal changes cause significant effects on the foraging activity and wood consumption of C. formosanus in New Orleans and how changes in specific climatic variables affect termite foraging behavior. There was a significant correlation between w...

  20. Energy harvesting from wood floor vibration using a piezoelectric generator

    Treesearch

    Jiangming Kan; Robert J. Ross; Xiping Wang; Wenbin Li

    2017-01-01

    Vibration can occur in wood floor systems as a consequence of a variety of human activities, ranging from common daily movements associated with individuals living in homes to high-intensity activities associated with sporting events that are held in large sports arenas. For example, the potential for harvesting energy from a wooden floor system in public buildings...

  1. Wood Products Other Building Materials Used in New Residential Construction in the United States

    Treesearch

    David B. McKeever; Joe Elling

    2015-01-01

    On average, new residential construction accounts for about one-third of all wood products consumed in the United States annually. During periods of robust housing activity, 45% or more of all wood products consumed are for new single-family and multifamily housing. This can fall to as low as 20% or less during times of economic recession. Unfortunately, 2012 was not...

  2. Bonding of wood fiber composites using a synthetic chelator-lignin activation system

    Treesearch

    D. Yelle; B. Goodell; D.J. Gardner; A. Amirbahman; P. Winistofer; S. Shaler

    2004-01-01

    Wood fibers, after thermo-mechanical pulping, have a high concentration of lignin on the outer surface of the fiber; the residual middle lamella of the woody cell wall. When wood fibers are oxidatively treated with a chelator produced by Gloeophyllum trabeum (a brown-rot fungus), in the presence of hydrogen peroxide (H2O2) and ferric iron (FeIII), free radicals are...

  3. Use of wood-based materials in beef bedded manure packs: 2. Effect on odorous volatile organic compounds, odor activity value, Escherichia coli, and nutrient concentrations

    USDA-ARS?s Scientific Manuscript database

    The objectives of this study were to determine the effects of three types of wood-based bedding materials (kiln-dried pine wood chips, dry cedar chips, and green cedar chips) and corn stover on concentration of odorous volatile organic compounds (VOC) and total Escherichia coli in bedded pack materi...

  4. MANAGING SOURCES TO REDUCE RISK IN AND AROUND THE INDOOR ENVIRONMENT: IMPACT OF COATINGS ON DISLODGEABLE ARSENIC ON THE SURFACES OF CCA-TREATED WOOD

    EPA Science Inventory

    Due to the potential for ingestion of chromated copper arsenate (CCA) by the hand-to-mouth activities of young children who contact CCA treated wood, EPA has used wipe samples to study the potential benefits of paint-like coatings on CCA treated wood. Citizens who may be concern...

  5. Recent activities in flame retardancy of wood-plastic composites at the Forest Products Laboratory

    Treesearch

    Robert H. White; Nicole M. Stark; Nadir Ayrilmis

    2011-01-01

    For a variety of reasons, wood-plastic composite (WPC) products are widely available for some building applications. In applications such as outdoor decking, WPCs have gained a significant share of the market. As an option to improve the efficient use of wood fiber, the USDA Forest Service, Forest Products Laboratory (FPL), has an extensive research program on WPCs....

  6. Antioxidant Properties of the Methanol Extract of the Wood and Pericarp of Caesalpinia decapetala

    PubMed Central

    Pawar, CR; Surana, SJ

    2010-01-01

    The antioxidant activities of the methanol extracts from the wood and pericarp of Caesalpinia decapetala (Roth) Alston (Caesalpiniaceae) were assessed in efforts to validate the herb. The antioxidant activity of the plant has been studied using its ability to scavenger DPPH, superoxide radicals, and nitric oxide radical along with its ability to inhibit lipid peroxidation. The antioxidant activity and phenolic content of the pericarp as determined by the DPPH, superoxide radical, nitric oxide radical, total phenols, the flavonoids, and total flavonols were higher than that of the wood. Analysis of plant extracts revealed a high amount of polyphenols and flavonoids suggesting a possible role of these phytoconstituents in the antioxidant property. Moreover, the results were observed in a concentration and dose dependent manner. Studies clearly indicate that the C. decapetala has significant antioxidant activity. PMID:21331190

  7. Resolving the smell of wood - identification of odour-active compounds in Scots pine (Pinus sylvestris L.).

    PubMed

    Schreiner, Linda; Bauer, Patrick; Buettner, Andrea

    2018-05-29

    Being one of the most common trees in forests, Pinus sylvestris L. is a frequently used raw material for wood products. Its specific odour is, however, mostly unresolved to date. Accordingly, we investigated Scots pine wood samples grown in Germany for their main odorant composition. We employed dedicated odorant analysis techniques such as gas chromatography-olfactometry (GC-O) and aroma extract dilution analysis (AEDA) and successfully detected 44 odour-active compounds; of these, 39 substances were successfully identified by gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and two-dimensional gas chromatography-mass spectrometry/olfactometry (2D-GC-MS/O). Among the main odorants found were (E,E)-nona-2,4-dienal, vanillin, phenylacetic acid, 3-phenylpropanoic acid, δ-octalactone and α-pinene, all of them having been detected with high flavour dilution factors during GC-O analyses. The majority of the identified odorants were fatty acid degradation products, plus some terpenoic substances and odorous substances resulting from the degradation of lignin. Although some of the detected substances have previously been reported as constituents of wood, 11 substances are reported here for the first time as odour-active compounds in wood, amongst them heptanoic acid, γ-octalactone, δ-nonalactone and (E,Z,Z)-trideca-2,4,7-trienal.

  8. Wood machining highlights, 1972 and 1973

    Treesearch

    Charles W. Mcmillin

    1975-01-01

    Important wood machining research published during 1972 and 1973 is highlighted to provide the reader with a concise summary of activity in 17 fields of endeavor. The review is based on 427 references and contains 154 citations.

  9. Wood Machining Highlights, 1972 and 1973

    Treesearch

    C.W. McMillin

    1975-01-01

    Important wood machining research published during 1972 and 1973 is highlighted to provide the reader with a concise summary of activity in 17 fields of endeavor. The review is based on 427 references and contains 154 citations.

  10. Chem-Is-Tree.

    ERIC Educational Resources Information Center

    Barry, Dana M.

    1997-01-01

    Provides details on the chemical composition of trees including a definition of wood. Also includes an activity on anthocyanins as well as a discussion of the resistance of wood to solvents and chemicals. Lists interesting products from trees. (DDR)

  11. Investigating the molecular underpinnings underlying morphology and changes in carbon partitioning during tension wood formation in Eucalyptus.

    PubMed

    Mizrachi, Eshchar; Maloney, Victoria J; Silberbauer, Janine; Hefer, Charles A; Berger, Dave K; Mansfield, Shawn D; Myburg, Alexander A

    2015-06-01

    Tension wood has distinct physical and chemical properties, including altered fibre properties, cell wall composition and ultrastructure. It serves as a good system for investigating the genetic regulation of secondary cell wall biosynthesis and wood formation. The reference genome sequence for Eucalyptus grandis allows investigation of the global transcriptional reprogramming that accompanies tension wood formation in this global wood fibre crop. We report the first comprehensive analysis of physicochemical wood property changes in tension wood of Eucalyptus measured in a hybrid (E. grandis × Eucalyptus urophylla) clone, as well as genome-wide gene expression changes in xylem tissues 3 wk post-induction using RNA sequencing. We found that Eucalyptus tension wood in field-grown trees is characterized by an increase in cellulose, a reduction in lignin, xylose and mannose, and a marked increase in galactose. Gene expression profiling in tension wood-forming tissue showed corresponding down-regulation of monolignol biosynthetic genes, and differential expression of several carbohydrate active enzymes. We conclude that alterations of cell wall traits induced by tension wood formation in Eucalyptus are a consequence of a combination of down-regulation of lignin biosynthesis and hemicellulose remodelling, rather than the often proposed up-regulation of the cellulose biosynthetic pathway. © 2014 University of Pretoria New Phytologist © 2014 New Phytologist Trust.

  12. Recent advances quantifying the large wood dynamics in river basins: New methods and remaining challenges

    NASA Astrophysics Data System (ADS)

    Ruiz-Villanueva, Virginia; Piégay, Hervé; Gurnell, Angela A.; Marston, Richard A.; Stoffel, Markus

    2016-09-01

    Large wood is an important physical component of woodland rivers and significantly influences river morphology. It is also a key component of stream ecosystems. However, large wood is also a source of risk for human activities as it may damage infrastructure, block river channels, and induce flooding. Therefore, the analysis and quantification of large wood and its mobility are crucial for understanding and managing wood in rivers. As the amount of large-wood-related studies by researchers, river managers, and stakeholders increases, documentation of commonly used and newly available techniques and their effectiveness has also become increasingly relevant as well. Important data and knowledge have been obtained from the application of very different approaches and have generated a significant body of valuable information representative of different environments. This review brings a comprehensive qualitative and quantitative summary of recent advances regarding the different processes involved in large wood dynamics in fluvial systems including wood budgeting and wood mechanics. First, some key definitions and concepts are introduced. Second, advances in quantifying large wood dynamics are reviewed; in particular, how measurements and modeling can be combined to integrate our understanding of how large wood moves through and is retained within river systems. Throughout, we present a quantitative and integrated meta-analysis compiled from different studies and geographical regions. Finally, we conclude by highlighting areas of particular research importance and their likely future trajectories, and we consider a particularly underresearched area so as to stress the future challenges for large wood research.

  13. Particles from wood smoke and road traffic differently affect the innate immune system of the lung.

    PubMed

    Samuelsen, Mari; Cecilie Nygaard, Unni; Løvik, Martinus

    2009-09-01

    The effect of particles from road traffic and wood smoke on the innate immune response in the lung was studied in a lung challenge model with the intracellular bacterium Listeria monocytogenes. Female Balb/cA mice were instilled intratracheally with wood smoke particles, particles from road traffic collected during winter (studded tires used; St+), and during autumn (no studded tires; St-), or diesel exhaust particles (DEP). Simultaneously with, and 1 or 7 days after particle instillation, 10(5) bacteria were inoculated intratracheally. Bacterial numbers in the lungs and spleen 1 day after Listeria challenge were determined, as an indicator of cellular activation. In separate experiments, bronchoalveolar lavage (BAL) fluid was collected 4 h and 24 h after particle instillation. All particles tested reduced the numbers of bacteria in the lung 24 h after bacterial inoculation. When particles were given simultaneously with Listeria, the reduction was greatest for DEP, followed by St+ and St-, and least for wood smoke particles. Particle effects were no longer apparent after 7 days. Neutrophil numbers in BAL fluid were increased for all particle exposed groups. St+ and St- induced the highest levels of IL-1beta, MIP-2, MCP-1, and TNF-alpha, followed by DEP, which induced no TNF-alpha. In contrast, wood smoke particles only increased lactate dehydrogenase (LDH) activity, indicating a cytotoxic effect of these particles. In conclusion, all particles tested activated the innate immune system as determined with Listeria. However, differences in kinetics of anti-Listeria activity and levels of proinflammatory mediators point to cellular activation by different mechanisms.

  14. Species associations overwhelm abiotic conditions to dictate the structure and function of wood-decay fungal communities.

    PubMed

    Maynard, Daniel S; Covey, Kristofer R; Crowther, Thomas W; Sokol, Noah W; Morrison, Eric W; Frey, Serita D; van Diepen, Linda T A; Bradford, Mark A

    2018-04-01

    Environmental conditions exert strong controls on the activity of saprotrophic microbes, yet abiotic factors often fail to adequately predict wood decomposition rates across broad spatial scales. Given that species interactions can have significant positive and negative effects on wood-decay fungal activity, one possibility is that biotic processes serve as the primary controls on community function, with abiotic controls emerging only after species associations are accounted for. Here we explore this hypothesis in a factorial field warming- and nitrogen-addition experiment by examining relationships among wood decomposition rates, fungal activity, and fungal community structure. We show that functional outcomes and community structure are largely unrelated to abiotic conditions, with microsite and plot-level abiotic variables explaining at most 19% of the total variability in decomposition and fungal activity, and 2% of the variability in richness and evenness. In contrast, taxonomic richness, evenness, and species associations (i.e., co-occurrence patterns) exhibited strong relationships with community function, accounting for 52% of the variation in decomposition rates and 73% in fungal activity. A greater proportion of positive vs. negative species associations in a community was linked to strong declines in decomposition rates and richness. Evenness emerged as a key mediator between richness and function, with highly even communities exhibiting a positive richness-function relationship and uneven communities exhibiting a negative or null response. These results suggest that community-assembly processes and species interactions are important controls on the function of wood-decay fungal communities, ultimately overwhelming substantial differences in abiotic conditions. © 2018 by the Ecological Society of America.

  15. Wood charcoal and activated carbon dust pneumoconiosis in three workers.

    PubMed

    De Capitani, Eduardo Mello; Algranti, Eduardo; Handar, Aantonieta M Z; Altemani, Albina M A; Ferreira, R G; Balthazar, Alipio Barbosa; Cerqueira, Elza Maria F P; Sanae Ota, Jaquelina

    2007-03-01

    Data on prevalence of lung diseases due to inhalation of carbonaceous materials other than mineral coal is very limited. We present three cases of wood charcoal pneumoconiosis, two due to activated carbon, and one from wood charcoal artisan handling. To our knowledge, no clinical cases of wood charcoal pneumoconiosis, from artisan handling has been published so far. The three cases had their X rays classified by two B-readers as p/q round opacities with profusion ranging from 2/2 to 3/3. HRCT of two of them showed a diffuse centrilobular ground glass nodular pattern with subpleural small areas of consolidations. Transbronchial biopsies showed deposition of black pigment in the bronchiolar interstice similar to the histological appearance of simple coal workers pneumoconiosis, with no signs of fibrosis. Spirometry showed no abnormalities in the three cases. The authors point out to a probably underestimated respiratory occupational risk related to wood charcoal manipulation, which must be addressed mostly in developing countries, where deficient workplace conditions can lead to exposure above limit levels. (c) 2007 Wiley-Liss, Inc.

  16. Temporal dynamics of instream wood in headwater streams draining mixed Carpathian forests

    NASA Astrophysics Data System (ADS)

    Galia, Tomáš; Šilhán, Karel; Ruiz-Villanueva, Virginia; Tichavský, Radek; Stoffel, Markus

    2017-09-01

    Instream wood can reside in fluvial systems over varying periods depending on its geographical context, instream position, tree species, piece size, and fluvial environment. In this paper, we investigate the residence time of two typical species representing a majority of instream wood in steep headwaters of the Carpathians and located under mixed forest canopy. Residence times of individual logs were then confronted with other wood parameters (i.e., wood dimensions, mean annual increment rate, tree age, class of wood stabilisation and decay, geomorphic function of wood pieces, and the proportion of the log length within the active channel). Norway spruce (Picea abies (L.) Karst.) samples indicated more than two times longer mean and maximal residence times as compared to European beech (Fagus sylvatica L.) based on the successful cross-dating of 127 logs. Maximum residence time in the headwaters was 128 years for P. abies and 59 years for F. sylvatica. We demonstrate that log age and log diameter played an important role in the preservation of wood in the fluvial system, especially in the case of F. sylvatica instream wood. By contrast, we did not observe any significant trends between wood residence time and total wood length. Instream wood with geomorphic functions (i.e., formation of steps and jams) did not show any differences in residence time as compared to nonfunctional wood. Nevertheless, we found shorter residence times for hillslope-stabilised pieces when compared to pieces located entirely in the channel (either unattached or stabilised by other wood or bed sediments). We also observed changes of instream wood orientation with respect to wood residence time. This suggests some movement of instream wood (i.e., its turning or short-distance transport), including pieces longer than channel width in the steep headwaters studied here (1.5 ≤ W ≤ 3.5 m), over the past few decades.

  17. Assessing the role of natural disturbance and forest management on dead wood dynamics in mixed-species stands of central Maine, USA

    Treesearch

    Joshua J. Puhlick; Aaron R. Weiskittel; Shawn Fraver; Matthew B. Russell; Laura S. Kenefic

    2016-01-01

    Dead wood pools are strongly influenced by natural disturbance events, stand development processes, and forest management activities. However, the relative importance of these influences can vary over time. In this study, we evaluate the role of these factors on dead wood biomass pools across several forest management alternatives after 60 years of treatment on the...

  18. The Pacific Basin market for wood products for military support activities

    Treesearch

    John D. Zinnikas

    1966-01-01

    Military support activities in Hawaii use between 50 and 150 thousand board feet of lumber annually for which locally grown and produced hardwood lumber might be used. In addition, the "other Pacific" market uses about 2 million board feet of hard-wood lumber annually. The Pacific Basin can be an important market for the Hawaii timber products industry...

  19. Authenticated DNA from Ancient Wood Remains

    PubMed Central

    LIEPELT, SASCHA; SPERISEN, CHRISTOPH; DEGUILLOUX, MARIE-FRANCE; PETIT, REMY J.; KISSLING, ROY; SPENCER, MATTHEW; DE BEAULIEU, JACQUES-LOUIS; TABERLET, PIERRE; GIELLY, LUDOVIC; ZIEGENHAGEN, BIRGIT

    2006-01-01

    • Background The reconstruction of biological processes and human activities during the last glacial cycle relies mainly on data from biological remains. Highly abundant tissues, such as wood, are candidates for a genetic analysis of past populations. While well-authenticated DNA has now been recovered from various fossil remains, the final ‘proof’ is still missing for wood, despite some promising studies. • Scope The goal of this study was to determine if ancient wood can be analysed routinely in studies of archaeology and palaeogenetics. An experiment was designed which included blind testing, independent replicates, extensive contamination controls and rigorous statistical tests. Ten samples of ancient wood from major European forest tree genera were analysed with plastid DNA markers. • Conclusions Authentic DNA was retrieved from wood samples up to 1000 years of age. A new tool for real-time vegetation history and archaeology is ready to use. PMID:16987920

  20. Interactions affect hyphal growth and enzyme profiles in combinations of coniferous wood-decaying fungi of Agaricomycetes

    PubMed Central

    Mali, Tuulia; Kuuskeri, Jaana; Shah, Firoz

    2017-01-01

    Fomitopsis pinicola is a species of Polyporales frequently encountered in Nordic temperate and boreal forests. In nature, the fungus causes destructive brown rot in wood, colonizing tree trunks often occupied by other Basidiomycota species. We mimicked these species-species interactions by introducing F. pinicola to five white rot species, all common saprotrophs of Norway spruce. Hyphal interactions and mycelial growth in various combinations were recorded, while activities of lignocellulose-acting CAZymes and oxidoreductases were followed in co-cultures on two different carbon-source media. Of the species, Phlebia radiata and Trichaptum abietinum were the strongest producers of lignin-modifying oxidoreductases (laccase, manganese peroxidase) when evaluated alone, as well as in co-cultures, on the two different growth media (low-nitrogen liquid medium containing ground coniferous wood, and malt extract broth). F. pinicola was an outstanding producer of oxalic acid (up to 61 mM), whereas presence of P. radiata prevented acidification of the growth environment in the liquid malt-extract cultures. When enzyme profiles of the species combinations were clustered, time-dependent changes were observed on wood-supplemented medium during the eight weeks of growth. End-point acidity and production of mycelium, oxalic acid and oxidoreductase activities, in turn clustered the fungal combinations into three distinct functional groups, determined by the presence of F. pinicola and P. radiata, by principal component analysis. Our findings indicate that combinations of wood-decay fungi have dramatic dynamic effects on the production of lignocellulose-active enzymes, which may lead to divergent degradative processes of dead wood and forest litter. PMID:28953947

  1. Deception Island, Antarctica, harbors a diverse assemblage of wood decay fungi.

    PubMed

    Held, Benjamin W; Blanchette, Robert A

    2017-02-01

    Very little is known about fungal diversity in Antarctica as compared to other biomes and how these important organisms function in this unusual ecosystem. Perhaps one of the most unusual ecosystems is that of Deception Island; an active volcanic island part of the South Shetland Islands of the Antarctic Peninsula. Here we describe the fungal diversity associated with historic wood from structures on the island, which reveals a diverse fungal assemblage of known wood decay fungi as well as the discovery of undescribed species. The major group of wood decay fungi identified were species of Cadophora and as shown in previous studies in other geographic regions of Antarctica, they caused a soft-rot type of decay in the introduced woods. Additionally, unlike other areas of Antarctica that have been studied, filamentous basidiomycetes (Hypochniciellum spp. and Pholiota spp.) were also identified that have different modes of degradation including brown and white rot. Matches of fungal sequences to known species in temperate regions likely introduced on building materials indicates human influences and volcanic activity have greatly impacted fungal diversity. Lahars (mudslides from volcanic activity) have partially buried many of the structures and the buried environment as well as the moist, warm soils provided conditions conducive for fungal growth that are not found in other regions of Antarctica. The diverse assemblage of decay fungi and different forms of wood decomposition add to the difficulty of conserving wooden structures at these important polar heritage sites. Copyright © 2016 British Mycological Society. Published by Elsevier Ltd. All rights reserved.

  2. Interactions affect hyphal growth and enzyme profiles in combinations of coniferous wood-decaying fungi of Agaricomycetes.

    PubMed

    Mali, Tuulia; Kuuskeri, Jaana; Shah, Firoz; Lundell, Taina Kristina

    2017-01-01

    Fomitopsis pinicola is a species of Polyporales frequently encountered in Nordic temperate and boreal forests. In nature, the fungus causes destructive brown rot in wood, colonizing tree trunks often occupied by other Basidiomycota species. We mimicked these species-species interactions by introducing F. pinicola to five white rot species, all common saprotrophs of Norway spruce. Hyphal interactions and mycelial growth in various combinations were recorded, while activities of lignocellulose-acting CAZymes and oxidoreductases were followed in co-cultures on two different carbon-source media. Of the species, Phlebia radiata and Trichaptum abietinum were the strongest producers of lignin-modifying oxidoreductases (laccase, manganese peroxidase) when evaluated alone, as well as in co-cultures, on the two different growth media (low-nitrogen liquid medium containing ground coniferous wood, and malt extract broth). F. pinicola was an outstanding producer of oxalic acid (up to 61 mM), whereas presence of P. radiata prevented acidification of the growth environment in the liquid malt-extract cultures. When enzyme profiles of the species combinations were clustered, time-dependent changes were observed on wood-supplemented medium during the eight weeks of growth. End-point acidity and production of mycelium, oxalic acid and oxidoreductase activities, in turn clustered the fungal combinations into three distinct functional groups, determined by the presence of F. pinicola and P. radiata, by principal component analysis. Our findings indicate that combinations of wood-decay fungi have dramatic dynamic effects on the production of lignocellulose-active enzymes, which may lead to divergent degradative processes of dead wood and forest litter.

  3. Habitat creation and biodiversity maintenance in mangrove forests: teredinid bivalves as ecosystem engineers

    PubMed Central

    Michie, Laura; Taylor, Ben W.

    2014-01-01

    Substantial amounts of dead wood in the intertidal zone of mature mangrove forests are tunnelled by teredinid bivalves. When the tunnels are exposed, animals are able to use tunnels as refuges. In this study, the effect of teredinid tunnelling upon mangrove forest faunal diversity was investigated. Mangrove forests exposed to long emersion times had fewer teredinid tunnels in wood and wood not containing teredinid tunnels had very few species and abundance of animals. However, with a greater cross-sectional percentage surface area of teredinid tunnels, the numbers of species and abundance of animals was significantly higher. Temperatures within teredinid-attacked wood were significantly cooler compared with air temperatures, and animal abundance was greater in wood with cooler temperatures. Animals inside the tunnels within the wood may avoid desiccation by escaping the higher temperatures. Animals co-existing in teredinid tunnelled wood ranged from animals found in terrestrial ecosystems including centipedes, crickets and spiders, and animals found in subtidal marine ecosystems such as fish, octopods and polychaetes. There was also evidence of breeding within teredinid-attacked wood, as many juvenile individuals were found, and they may also benefit from the cooler wood temperatures. Teredinid tunnelled wood is a key low-tide refuge for cryptic animals, which would otherwise be exposed to fishes and birds, and higher external temperatures. This study provides evidence that teredinids are ecosystem engineers and also provides an example of a mechanism whereby mangrove forests support intertidal biodiversity and nurseries through the wood-boring activity of teredinids. PMID:25276505

  4. Simulated long-term effects of varying tree retention on wood production, dead wood and carbon stock changes.

    PubMed

    Santaniello, Francesca; Djupström, Line B; Ranius, Thomas; Weslien, Jan; Rudolphi, Jörgen; Sonesson, Johan

    2017-10-01

    Boreal forests are an important source of timber and pulp wood, but provide also other products and services. Utilizing a simulation program and field data from a tree retention experiment in a Scots pine forest in central Sweden, we simulated the consequences during the following 100 years of various levels of retention on production of merchantable wood, dead wood input (as a proxy for biodiversity), and carbon stock changes. At the stand level, wood production decreased with increased retention levels, while dead wood input and carbon stock increased. We also compared 12 scenarios representing a land sharing/land sparing gradient. In each scenario, a constant volume of wood was harvested with a specific level of retention in a 100-ha landscape. The area not needed to reach the defined volume was set-aside during a 100-year rotation period, leading to decreasing area of set-asides with increasing level of retention across the 12 scenarios. Dead wood input was positively affected by the level of tree retention whereas the average carbon stock decreased slightly with increasing level of tree retention. The scenarios will probably vary in how they favor species preferring different substrates. Therefore, we conclude that a larger variation of landscape-level conservation strategies, also including active creation of dead wood, may be an attractive complement to the existing management. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Effects of nutrient enrichment on the decomposition of wood and associated microbial activity in streams

    Treesearch

    Vladislav Gulis; Amy D. Rosemond; Keller Suberkropp; Holly S. Weyers; Jonathan P. Benstead

    2004-01-01

    We determined the effects of nutrient enrichment on wood decomposition rates and microbial activity during a 3-year study in two headwater streams at Coweeta Hydrologic Laboratory, NC, U.S.A. After a 1-year pretreatment period, one of the streams was continuously enriched with inorganic nutrients (nitrogen and phosphorus) for 2 years while the other stream served as a...

  6. Estimated Softwood Volume Available for Wood Chipmills or other Low Grade Uses.

    EPA Pesticide Factsheets

    The potential of wood chip mills to influence the distribution of harvests and the dynamics of wood fiber utilization has become an issue of concern. Where wood chip mills are active in a timber market, they may increase the utilization of wood fiber derived from a given harvest; change the pattern and distribution of harvests on the landscape; and reduce production costs of fiber supply, thus increasing overall quantity supplied in a price-taking market. Wood chip mills have proliferated in the South in recent decades. This data layer was created to address the following question: If the Mid-Atlantic region behaves as the South, which areas are relatively more attractive as sources of low grade softwood fiber, and therefore relatively more vulnerable to any negative stresses associated with wood chip harvests? The data layer simulates annual softwood wood chip volume (in tons) available to supply chip mills or other low grade uses, if timber supply in the Mid-Atlantic mirrors trends for the South. More information about these resources, including the variables used in this study, may be found here: https://edg.epa.gov/data/Public/ORD/NERL/ReVA/ReVA_Data.zip.

  7. Co-culturing Effects of Coexisting Bacteria on Wood Degradation by Trametes versicolor.

    PubMed

    Kamei, Ichiro

    2017-01-01

    White-rot fungi are the main decomposers of wood cell-wall polymer in forest ecosystems. Little is known, however, about the interactions between white-rot fungi and other coexisting microorganisms in decayed wood. A white-rot fungus, Trametes versicolor strain TN6F, was isolated from a fruit body, and 44 strains of coexisting cultivable bacteria were isolated from its substrate, natural white rot-decayed wood. The effects of these bacteria on fungal growth were examined by an in vitro confrontation growth assay. Among the isolates, nine bacterial strains inhibited the growth of strain TN6F, while 35 strains did not affect the growth of TN6F. However, when co-cultured with strain TN6F on wood powder, many bacterial strains promoted the weight loss of the substrate. A subsequent chemical composition analysis showed that co-culturing accelerated delignification. Higher laccase activity was detected when strain TN6F was co-cultured on wood powder medium with bacterial strains TN6W-26 or TN6W-27. These results indicate that some bacterial strains might promote wood degradation.

  8. Changes in the distribution of radiocesium in the wood of Japanese cedar trees from 2011 to 2013.

    PubMed

    Ogawa, Hideki; Hirano, Yurika; Igei, Shigemitsu; Yokota, Kahori; Arai, Shio; Ito, Hirohisa; Kumata, Atsushi; Yoshida, Hirohisa

    2016-09-01

    The changes in the distribution of (137)Cs in the wood of Japanese cedar (Cryptomeria japonica) trunks within three years after the Fukushima Dai-ichi Nuclear Power Plant (FDNP) accident in 2011 were investigated. Thirteen trees were felled to collect samples at 6 forests in 2 regions of the Fukushima prefecture. The radial distribution of (137)Cs in the wood was measured at different heights. Profiles of (137)Cs distribution in the wood changed considerably from 2011 to 2013, and the process of (137)Cs distribution change in the wood was clarified. From 2011 to 2012, the active transportation from sapwood to heartwood and the radial diffusion in heartwood proceeded quickly, and the radial (137)Cs distribution differed according to the vertical positon of trees. From 2012 to 2013, the vertical diffusion of (137)Cs from the treetop to the ground, probably caused by the gradient of (137)Cs concentration in the trunk, was observed. Eventually, the radial (137)Cs distributions were nearly identical at any vertical positions in 2013. Our results suggested that the active transportation from sapwood to heartwood and the vertical and radial diffusion in heartwood proceeded according to the vertical position of the tree and (137)Cs distribution in the wood approached the equilibrium state within three years after the accident. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. POLLUTION PREVENTION CASE STUDIES: LOW-VOC/HAP WOOD FURNITURE COATINGS

    EPA Science Inventory

    This article provides a brief profile of the wood furniture industry, discusses pollution prevention activities typically implemented, describes the four low-VOC/HAP coating technologies studied. and summarizes one case study for each of the low-VOC/HAP coating yechnologies inves...

  10. Effect of Steam Activation on Development of Light Weight Biomorphic Porous SiC from Pine Wood Precursor

    NASA Astrophysics Data System (ADS)

    Manocha, Satish M.; Patel, Hemang; Manocha, L. M.

    2013-02-01

    Biomorphic SiC materials with tailor-made microstructure and properties similar to ceramic materials manufactured by conventional method are a new class of materials derived from natural biopolymeric cellulose templates (wood). Porous silicon carbide (SiC) ceramics with wood-like microstructure have been prepared by carbothermal reduction of charcoal/silica composites at 1300-1600 °C in inert Ar atmosphere. The C/SiO2 composites were fabricated by infiltrating silica sol into porous activated biocarbon template. Silica in the charcoal/silica composite, preferentially in the cellular pores, was found to get transformed in forms of fibers and rods due to shrinkage during drying. The changes in the morphology of resulting porous SiC ceramics after heat treatment to 1600 °C, as well as the conversion mechanism of wood to activated carbon and then to porous SiC ceramic have been investigated using scanning electron microscope, x-ray diffraction, thermogravimetric analysis, and differential scanning calorimetry. Activation of carbon prior to silica infiltration has been found to enhance conversion of charcoal to SiC. The pore structure is found to be uniform in these materials than in those made from as-such charcoal/silica composites. This provides a low-cost and eco-friendly route to advanced ceramic materials, with near-net shape potential.

  11. Household wood heater usage and indoor leakage of BTEX in Launceston, Australia: A null result

    NASA Astrophysics Data System (ADS)

    Galbally, Ian E.; Gillett, Robert W.; Powell, Jennifer C.; Lawson, Sarah J.; Bentley, Simon T.; Weeks, Ian A.

    A study has been conducted in Launceston, Australia, to determine within households with wood heaters the effect of leakage from the heater and flue on the indoor air concentrations of the pollutants: benzene, toluene, ethylbenzene and xylene (BTEX). The study involved three classes: 28 households without wood heaters, 19 households with wood heaters compliant with the relevant Australian Standard and 30 households with non-compliant wood heaters. Outdoor and indoor BTEX concentrations were measured in each household for 7 days during summer when there was little or no wood heater usage, and for 7 days during winter when there was widespread wood heater usage. Each participant kept a household activity diary throughout their sampling periods. For wintertime, there were no significant differences of the indoor BTEX concentrations between the three classes of households. Also there were no significant relationships between BTEX indoor concentrations within houses and several measures of the amount of wood heater use within these houses. For the households sampled in this study, the use of a wood heater within a house did not lead to BTEX release within that house and had no direct detectable influence on the concentrations of BTEX within the house. We propose that the pressure differences associated with the both the leakiness or permeability of the building envelope and the draught of the wood heater have key roles in determining whether there will be backflow of smoke from the wood heater into the house. For a leaky house with a well maintained wood heater there should be no backflow of smoke from the wood heater into the house. However backflow of smoke may occur in well sealed houses. The study also found that wood heater emissions raise the outdoor concentrations of BTEX in winter in Launceston and through the mixing of outdoor air through the building envelopes into the houses, these emissions contribute to increases in the indoor concentrations of BTEX in winter in all houses in Launceston.

  12. Overview of IEA biomass combustion activities

    NASA Astrophysics Data System (ADS)

    Hustad, J. E.

    1994-07-01

    The objectives of the International Energy Agency (IEA) bioenergy program are: (1) to encourage cooperative research, development and use of energy and the increased utilization of alternatives to oil; and (2) to establish increased program and project cooperation between participants in the whole field of bioenergy. There are four Task Annexes to the Implementing Agreement during the period 1992-1994: Efficient and Environmentally Sound Biomass Production Systems; Harvesting and Supply of Woody Biomass for Energy; Biomass Utilization; and Conversion of Municipal Solid Waste Feedstock to Energy. The report describes the following biomass combustion activities during the period 1992-1994: Round robin test of a wood stove; Emissions from biomass combustion; A pilot project cofiring biomass with oil to reduce SO2 emissions; Small scale biomass chip handling; Energy from contaminated wood waste combustion; Modeling of biomass combustion; Wood chip cogeneration; Combustion of wet biomass feedstocks, ash reinjection and carbon burnout; Oxidation of wet biomass; Catalytic combustion in small wood burning appliances; Characterization of biomass fuels and ashes; Measurement techniques (FTIR).

  13. Effect of artificial ageing using different wood chips on the antioxidant activity, resveratrol and catechin concentration, sensory properties and colour of two Greek red wines.

    PubMed

    Gortzi, Olga; Metaxa, Xenia; Mantanis, George; Lalas, Stavros

    2013-12-01

    Two Greek red wines (Syrah and Cabernet) were artificially aged with different wood chips (white oak, red oak, Turkey oak, chestnut, Bosnian pine, cherry, common juniper, common walnut, white mulberry, black locust and apricot). The influence of each wood species was tested for up to 20 days. The optimum duration for the extraction of total polyphenols was 20 days (Syrah) or 10 days (Cabernet) when chips of white oak, chestnut, cherry, white mulberry, black locust and apricot where used. Resveratrol and catechin concentrations ranged within the limits previously reported in literature. A high antioxidant activity was established after 10 days of artificial ageing. The sensory evaluation showed that the best results were produced by the apricot chips after 5 days (Syrah) or black locust and apricot after 5 days (Cabernet). Colour was seen to increase with both time of ageing and number of wood chips added. Copyright © 2013 Elsevier Ltd. All rights reserved.

  14. BOREAS TE-2 Wood Respiration Data

    NASA Technical Reports Server (NTRS)

    Ryan, Michael G.; Lavigne, Michael; Hall, Forrest G. (Editor); Papagno, Andrea (Editor)

    2000-01-01

    The BOREAS TE-2 team collected several data sets in support of its efforts to characterize and interpret information on the respiration of the foliage, roots, and wood of boreal vegetation. This data set contains measurements of wood respiration conducted in the NSA during the growing season of 1994. The data are stored in tabular ASCII files. The data files are available on a CD-ROM (see document number 20010000884), or from the Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC).

  15. BOREAS TE-2 Continuous Wood Respiration Data

    NASA Technical Reports Server (NTRS)

    Hall, Forrest G. (Editor); Papagno, Andrea (Editor); Ryan, Michael G.; Lavigne, Michael

    2000-01-01

    The BOREAS TE-2 team collected several data sets in support of its efforts to characterize and interpret information on the respiration of the foliage, roots, and wood of boreal vegetation. This data set contains measurements of wood respiration measured continuously (about once per hour) in the NSA during the growing season of 1994. The data are stored in tabular ASCII files. The data files are available on a CD-ROM (see document number 20010000884), or from the Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC).

  16. Production of a COX-2 inhibitor, 2,4,5-trimethoxybenzaldehyde, with submerged cultured Antrodia camphorata.

    PubMed

    Chen, C-C; Chyau, C-C; Hseu, T-H

    2007-04-01

    To investigate the active ingredient in fruiting bodies and to produce it with cultured mycelium in Antrodia camphorata (BCRC 35398). The volatile components from the fruiting bodies, the liquid cultured broth of A. camphorata and Cinnamomum kanehirae wood were separately isolated by steam distillation-solvent extraction and identified by gas chromatography-mass spectrometry. In the fruiting bodies, a COX-2 inhibitor 2,4,5-trimethoxybenzaldehyde (TMBA) was found to be the most abundant constituent, but was totally absent in its cultured broth and its natural host, C. kanehirae wood. On feeding with the acid-digested sawdust of C. kanehirae wood or vanillin to the broth for culture, TMBA was produced in both cultured broths. The TMBA identified in fruiting bodies was an active ingredient whose functions consisted with the reported experiences of this mushroom. Feeding vanillin to culture broth could produce TMBA containing mycelium product like its fruiting bodies did. This study found an active ingredient in fruiting bodies of A. camphorata and elucidated this compound derived from digested sawdust of C. kanehirae wood. A feasible method was also developed to produce TMBA containing mycelium by feeding vanillin.

  17. Wood phenology, not carbon input, controls the interannual variability of wood growth in a temperate oak forest.

    PubMed

    Delpierre, Nicolas; Berveiller, Daniel; Granda, Elena; Dufrêne, Eric

    2016-04-01

    Although the analysis of flux data has increased our understanding of the interannual variability of carbon inputs into forest ecosystems, we still know little about the determinants of wood growth. Here, we aimed to identify which drivers control the interannual variability of wood growth in a mesic temperate deciduous forest. We analysed a 9-yr time series of carbon fluxes and aboveground wood growth (AWG), reconstructed at a weekly time-scale through the combination of dendrometer and wood density data. Carbon inputs and AWG anomalies appeared to be uncorrelated from the seasonal to interannual scales. More than 90% of the interannual variability of AWG was explained by a combination of the growth intensity during a first 'critical period' of the wood growing season, occurring close to the seasonal maximum, and the timing of the first summer growth halt. Both atmospheric and soil water stress exerted a strong control on the interannual variability of AWG at the study site, despite its mesic conditions, whilst not affecting carbon inputs. Carbon sink activity, not carbon inputs, determined the interannual variations in wood growth at the study site. Our results provide a functional understanding of the dependence of radial growth on precipitation observed in dendrological studies. © 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

  18. Phenolic Components and Antioxidant Activity of Wood Extracts from 10 Main Spanish Olive Cultivars.

    PubMed

    Salido, Sofía; Pérez-Bonilla, Mercedes; Adams, Robert P; Altarejos, Joaquín

    2015-07-29

    The chemical composition and radical-scavenging activity of wood samples from 10 main Spanish olive cultivars were studied. The wood samples were collected during the pruning works from trees growing under the same agronomical and environmental conditions. The 10 ethyl acetate extracts were submitted to HPLC-DAD/ESI-MS analysis to determine the phenolic constituents. Seventeen compounds were identified (10 secoiridoids, 3 lignans, 2 phenol alcohols, 1 iridoid, and 1 flavonoid) by comparison with authentic samples. Significant quantitative and qualitative differences were found among olive cultivars. The lignan (+)-1-hydroxypinoresinol 1-O-β-d-glucopyranoside was the major compound in all olive cultivars, except in cultivars 'Farga' and 'Picual'. The multivariate analysis of all data revealed three sets of cultivars with similar compositions. Cultivars 'Gordal sevillana' and 'Picual' had the most distinct chemical profiles. With regard to the radical-scavenging activity, cultivar 'Picual', with oleuropein as the major phenolic, showed the highest activity (91.4 versus 18.6-32.7%).

  19. Mixture toxicity of wood preservative products in the fish embryo toxicity test.

    PubMed

    Coors, Anja; Dobrick, Jan; Möder, Monika; Kehrer, Anja

    2012-06-01

    Wood preservative products are used globally to protect wood from fungal decay and insects. We investigated the aquatic toxicity of five commercial wood preservative products, the biocidal active substances and some formulation additives contained therein, as well as six generic binary mixtures of the active substances in the fish embryo toxicity test (FET). Median lethal concentrations (LC50) of the single substances, the mixtures, and the products were estimated from concentration-response curves and corrected for concentrations measured in the test medium. The comparison of the experimentally observed mixture toxicity with the toxicity predicted by the concept of concentration addition (CA) showed less than twofold deviation for all binary mixtures of the active substances and for three of the biocidal products. A more than 60-fold underestimation of the toxicity of the fourth product by the CA prediction was detected and could be explained fully by the toxicity of one formulation additive, which had been labeled as a hazardous substance. The reason for the 4.6-fold underestimation of toxicity of the fifth product could not be explained unambiguously. Overall, the FET was found to be a suitable screening tool to verify whether the toxicity of formulated wood preservatives can reliably be predicted by CA. Applied as a quick and simple nonanimal screening test, the FET may support approaches of applying component-based mixture toxicity predictions within the environmental risk assessment of biocidal products, which is required according to European regulations. Copyright © 2012 SETAC.

  20. Assessment of flow forces on large wood in rivers

    USDA-ARS?s Scientific Manuscript database

    Large wood (LW) exerts an important influence on the geomorphology and ecology of streams and rivers. LW management activities are diverse, including placement in streams for restoring habitats or controlling bank erosion and mitigation of LW-related hazards to bridges and other structures. Flow f...

  1. Hazardous Waste Cleanup: Boricua Wood Processing Incorporated in Toja Baja, Puerto Rico

    EPA Pesticide Factsheets

    Boricua Wood Processing, Inc. is located on State Road 865 at kilometer 5.5, in Toja Baja. The facility is a manufacturing plant for the pressure injection of liquid preservative solutions into untreated cut lumber. The facility began its activity in 1957.

  2. Tyrosinase inhibitors from the wood of Artocarpus heterophyllus.

    PubMed

    Nguyen, Nhan Trung; Nguyen, Mai Ha Khoa; Nguyen, Hai Xuan; Bui, Ngan Kim Nguyen; Nguyen, Mai Thanh Thi

    2012-11-26

    From the methanolic-soluble extract of the wood of Artocarpus heterophyllus, four new flavones, artocarmins A-D (1-4), and three new chalcones, artocarmitins A-C (5-7), have been isolated together with 13 known compounds. Their structures were determined on the basis of the spectroscopic data. Compounds 1-4, 6, 7, 9-16, and 20 displayed significant tyrosinase inhibitory activity. The most active compound, morachalcone A (12) (IC50, 0.013 μM), was 3000 times more active as a tyrosinase inhibitor than a positive control, kojic acid (IC50, 44.6 μM).

  3. The Long-Term Effects of Large Wood Placement on Salmonid Habitat in East Fork Mill Creek, Redwood National and State Park, California

    NASA Astrophysics Data System (ADS)

    Rodriguez, D. L.; Stubblefield, A. P.

    2017-12-01

    The conservation and recovery of anadromous salmonids (Oncorhynchus sp.) depend on stream restoration and protection of freshwater habitats. Instream large wood dictates channel morphology, increase retention of terrestrial inputs such as organic matter, nutrients and sediment, and enhances the quality of fish habitat. Historic land use/land cover changes have resulted in aquatic systems devoid of this component. Restoration by placement of large wood jams is intended to restore physical and biological processes. An important question for scientists and managers, in addition to the initial effectiveness of restoration, is the persistence and fate of this type of project. In this study we compare channel change and large wood attributes on the East Fork of Mill Creek, a tributary of the Smith River in northern California, eight years after a major instream wood placement effort took place. Our results are compared with previously published data from before and one year after the restoration. Preliminary results suggest the dramatic increase in spawning gravel abundance and large wood accumulation observed in the earlier study have persisted. From 2008 to 2016 a reduction in median sediment size, ranging from 103-136 percent, has been observed in a majority of the sites. The sites have continued to grow in size and influence by racking floating wood from upstream and destabilizing proximate banks of riparian alder, increasing both instream large wood volume (5-196 %) and floodplain connectivity. Preliminary results also show a decrease in residual pool depth and an increase in pool length which may be attributed to floodplain connectivity. Changes to the following attributes are evaluated: 1) wood loading (total site wood volume, total wood volume in active channel, and wood piece count); 2) percent pool cover by large wood; 3) residual pool depth; 4) upstream sediment aggradation; 5) floodplain connectivity; and 6) mean sediment size directly above and below large wood. We present on these results and statistical comparisons of total site wood volume with response factors.

  4. A survey of size-fractionated dust levels in the U.S. wood processing industry.

    PubMed

    Kalliny, Medhat I; Brisolara, Joseph A; Glindmeyer, Henry; Rando, Roy

    2008-08-01

    A survey of size-fractionated dust exposure was carried out in 10 wood processing plants across the United States as part of a 5-year longitudinal respiratory health study. The facilities included a sawmill, plywood assembly plants, secondary wood milling operations, and factories producing finished wood products such as wood furniture and cabinets. Size-fractionated dust exposures were determined using the RespiCon Personal Particle Sampler. There were 2430 valid sets of respirable, thoracic, and inhalable dust samples collected. Overall, geometric mean (geometric standard deviation) exposure levels were found to be 1.44 (2.67), 0.35 (2.65), and 0.18 (2.54) mg/m, for the inhalable, thoracic, and respirable fractions, respectively. Averaged across all samples, the respirable fraction accounted for 16.7% of the inhalable dust mass, whereas the corresponding figure for thoracic fraction as a percentage of the inhalable fraction was 28.7%. Exposures in the furniture manufacturing plants were significantly higher than those in sawmill and plywood assembly plants, wood milling plants, and cabinet manufacturing plants, whereas the sawmill and plywood assembly plants exhibited significantly lower dust levels than the other industry segments. Among work activities, cleaning with compressed air and sanding processes produced the highest size-fractionated dust exposures, whereas forklift drivers demonstrated the lowest respirable and inhalable dust fractions and shipping processes produced the lowest thoracic dust fraction. Other common work activities such as sawing, milling, and clamping exhibited intermediate exposure levels, but there were significant differences in relative ranking of these across the various industry segments. Processing of hardwood and mixed woods generally were associated with higher exposures than were softwood and plywood, although these results were confounded with industry segment also.

  5. Movement of Xylosandrus germanus (Coleoptera: Curculionidae) in Ornamental Nurseries and Surrounding Habitats.

    PubMed

    Reding, Michael E; Ranger, Christopher M; Sampson, Blair J; Werle, Christopher T; Oliver, Jason B; Schultz, Peter B

    2015-08-01

    Some exotic ambrosia beetles are damaging pests in ornamental nurseries. Xylosandrus germanus (Blandford) is the most problematic ambrosia beetle in Ohio nurseries. Movement of X. germanus in nurseries has not been characterized, and knowledge is lacking on whether infestations originate from within nurseries or surrounding habitats. Flight activity of X. germanus was monitored in nurseries and adjacent wooded areas to determine the source of beetles infesting nurseries, and characterize their movement within nurseries. Ethanol-baited bottle traps were positioned within wooded areas adjacent to commercial nurseries and within nurseries at various distances from the nursery woodlot interface. Flight activity of overwintered X. germanus occurred in wooded areas adjacent to nurseries before occurrence within nurseries. There was a direct relationship between degree-days and the distance from woodlots when X. germanus were first found in traps in spring, with earlier captures closest to wooded areas and latest ones furthest away into the nursery. X. germanus appeared to move into nurseries from adjacent wooded areas, with numbers trapped within nurseries decreasing with distance away from wooded areas. Trees in the interior of nurseries would appear to be subjected to less attack pressure than trees near the nursery border. Intercepting beetles as they move into nurseries might be an effective strategy to reduce attack pressure on valuable trees. Published by Oxford University Press on behalf of Entomological Society of America 2015. This work is written by US Government employees and is in the public domain in the US.

  6. Natural decomposition of hornbeam wood decayed by the white rot fungus Trametes versicolor.

    PubMed

    Karim, Maryam; Daryaei, Mehrdad Ghodskhah; Torkaman, Javad; Oladi, Reza; Ghanbary, Mohammad Ali Tajick; Bari, Ehsan; Yilgor, Nural

    2017-01-01

    The impacts of white-rot fungi on altering wood chemistry have been studied mostly in vitro. However, in vivo approaches may enable better assessment of the nature of interactions between saprotrophic fungi and host tree in nature. Hence, decayed and sound wood samples were collected from a naturally infected tree (Carpinus betulus L.). Fruiting bodies of the white rot fungus Trametes versicolor grown on the same tree were identified using rDNA ITS sequencing. Chemical compositions (cellulose and lignin) of both sound and infected wood were studied. FT-IR spectroscopy was used to collect spectra of decayed and un-decayed wood samples. The results of chemical compositions indicated that T. versicolor reduced cellulose and lignin in similar quantities. Fungal activities in decayed wood causes serious decline in pH content. The amount of alcohol-benzene soluble extractives was severely decreased, while a remarkable increase was found in 1% sodium hydroxide soluble and hot water extractive contents in the decayed wood samples, respectively. FT-IR analyses demonstrated that T. versicolor causes simultaneous white rot in the hornbeam tree in vivo which is in line with in vitro experiments.

  7. Combination of sawdust from teak wood and rice husk activated carbon as adsorbent of Pb(II) ion and its analysis using solid-phase spectrophotometry (sps)

    NASA Astrophysics Data System (ADS)

    Saputro, S.; Mahardiani, L.; Wulandari, D. A.

    2018-03-01

    This research aimed to know the usage of sawdust of teak wood and rice husk waste as Pb (II) ion adsorbents in simulated liquid waste, the combined optimum mass required adsorbent to adsorb Pb(II) ion, the sensitivity of the solid-phase spectrophotometry (sps) method in determining the decrease of Pb (II) metal ion levels in the μg/L level. This research was conducted by experimental method in laboratory. Adsorbents used in this study were charcoal of sawdust sawdust activated using 15% ZnCl2 solution and activated rice husk using 2 N NaOH solution. The adsorption processes of sawdust and rice husk with Pb(II) solution was done by variation of mass combination with a ratio of 1: 0; 0: 1; 1: 1; 1: 2; and 2: 1. Analysis of Pb(II) ion concentration using SPS and characterization of sawdust and rice husk adsorbent ads using FTIR. The results showed that activated charcoal from sawdust of teak wood and rice husks can be used as Pb (II) metal ion adsorbents with adsorption capacity of 0.86 μg/L, charcoal from sawdust of teak wood and rice husk adsorbent with a combination of optimum mass contact of sawdust and rice husk is 2:1 as much as 3 grams can adsorb 42.80 μg/L. Solid-phase spectophotometry is a sensitive method for analysis of concentration decreasing levels of Pb(II) ion, after it was absorbed by sawdust of teak wood and rice husk with high sensitivity and has the limit of detection (LOD) of 0.06 μg/L.

  8. Antimicrobial activity, cytotoxicity and DNA binding studies of carbon dots

    NASA Astrophysics Data System (ADS)

    Jhonsi, Mariadoss Asha; Ananth, Devanesan Arul; Nambirajan, Gayathri; Sivasudha, Thilagar; Yamini, Rekha; Bera, Soumen; Kathiravan, Arunkumar

    2018-05-01

    In recent years, quantum dots (QDs) are one of the most promising nanomaterials in life sciences community due to their unexploited potential in biomedical applications; particularly in bio-labeling and sensing. In the advanced nanomaterials, carbon dots (CDs) have shown promise in next generation bioimaging and drug delivery studies. Therefore the knowledge of the exact nature of interaction with biomolecules is of great interest to designing better biosensors. In this study, the interaction between CDs derived from tamarind and calf thymus DNA (ct-DNA) has been studied by vital spectroscopic techniques, which revealed that the CDs could interact with DNA via intercalation. The apparent association constant has been deduced from the absorption spectral changes of ct-DNA-CDs using the Benesi-Hildebrand equation. From the DNA induced emission quenching experiments the apparent DNA binding constant of the CDs (Kapp) have also been evaluated. Furthermore, we have analyzed the antibacterial and antifungal activity of CDs using disc diffusion assay method which exhibited excellent activity against E. coli and C. albicans with inhibition zone in the range of 7-12 mm. The biocompatible nature of CDs was confirmed by an in vitro cytotoxicity test on L6 normal rat myoblast cells by using MTT assay. The cell viability is not affected till the high dosage of CDs (200 μg/mL) for >48 h. As a consequence of the work, future development of CDs for microbial control and DNA sensing among the various biomolecules is possible in view of emerging biofields.

  9. The association between chromaticity, phenolics, carotenoids, and in vitro antioxidant activity of frozen fruit pulp in Brazil: an application of chemometrics.

    PubMed

    Zielinski, Acácio Antonio Ferreira; Ávila, Suelen; Ito, Vivian; Nogueira, Alessandro; Wosiacki, Gilvan; Haminiuk, Charles Windson Isidoro

    2014-04-01

    A total of 19 Brazilian frozen pulps from the following fruits: açai (Euterpe oleracea), blackberry (Rubus sp.), cajá (Spondias mombin), cashew (Anacardium occidentale), cocoa (Theobroma cacao), coconut (Cocos nucifera), grape (Vitis sp.), graviola (Annona muricata), guava (Psidium guajava), papaya (Carica papaya), peach (Prunus persica), pineapple (Ananas comosus), pineapple and mint (A. comosus and Mentha spicata), red fruits (Rubus sp. and Fragaria sp.), seriguela (Spondias purpurea), strawberry (Fragaria sp.), tamarind (Tamarindus indica), umbu (Spondias tuberosa), and yellow passion fruit (Passiflora edulis) were analyzed in terms of chromaticity, phenolic compounds, carotenoids, and in vitro antioxidant activity using ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. Data were processed using principal component analysis (PCA) and hierarchical cluster analysis (HCA). Antioxidant capacity was measured by DPPH and FRAP assays, which showed significant (P < 0.01) correlation with total phenolic compounds (r = 0.88 and 0.70, respectively), total flavonoids (r = 0.63 and 0.81, respectively), and total monomeric anthocyanins (r = 0.59 and 0.73, respectively). PCA explained 74.82% of total variance of data, and the separation into 3 groups in a scatter plot was verified. Three clusters also suggested by HCA, corroborated with PCA, in which cluster 3 was formed by strawberry, red fruits, blackberry, açaí, and grape pulps. This cluster showed the highest contents of total phenolic compounds, total flavonoids, and antioxidant activity. © 2014 Institute of Food Technologists®

  10. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Whitford, W.G.; Elkins, N.Z.; Parker, L.W.

    In laboratory microcosms of coal mine spoil amended with bark and wood chips, the activity of termites increased organic matter and increased total nitrogen. Termite survival was reduced in microcosms with spoil and paper or straw amendments. Field studies evaluating the efficacy of organic amendments in developing a soil biota showed that decomposition rates of wood chip-bark amended spoil were the same as unmined soil and that decomposition rates were lower than all other mulch-spoil combinations. Wood and bark amended-spoil had the highest density and diversity of soil fauna. Top dressing spoils with borrow soil did not improve any ofmore » the soil biological parameters measured. Based on these data it was recommended that reclamation procedures be changed to eliminate borrow soil top-dressing and that wood removed from mined areas be returned to the contoured spoil as wood chip amendment in addition to straw mulch.« less

  11. Engineered and Other Wood Products - An Opportunity to "Grow the Pie"

    Treesearch

    Albert Schuler; Craig Adair

    2003-01-01

    The market for engineered wood products (EWP) is being driven by a number of factors including: technology; reduced availability of old-growth timber; construction activity, and globalization. Specifically, technological developments have allowed the industry to "engineer" or "design" improved performance properties and to utilize former "weed...

  12. FOREST HARVESTS AND WOOD PRODUCTS: SOURCES AND SINKS OF ATMOSPHERIC CARBON DIOXIDE

    EPA Science Inventory

    Changes in the net carbon(c)sink-source balance related to a country's forest harvesting and use of wood products is an important component in making country-level inventories of greenhouse gas emissions,a current activity within many signatory nations to the UN Framework Convent...

  13. Commercial activities and subsistence utilization of mangrove forests around the Wouri estuary and the Douala-Edea reserve (Cameroon).

    PubMed

    Atheull, Adolphe Nfotabong; Din, Ndongo; Longonje, Simon N; Koedam, Nico; Dahdouh-Guebas, Farid

    2009-11-17

    Worldwide there is growing research interest in the ethnobiology of mangrove forests. Notwithstanding that, little information has been published about ethnobiology of mangrove forests in Cameroon. The aims of this study were a) to analyze the harvesting methods and the local selling of mangrove wood products by loggers in the vicinity of Wouri estuary and b) to investigate the patterns of subsistence uses of mangrove wood products around the Douala-Edea reserve. Semi-structured interviews were conducted with 120 active mangrove loggers in 23 Douala wood markets and 103 households located in three villages (Mbiako, Yoyo I and Yoyo II) close to Douala-Edea reserve. In each of the three densely populated villages, every second household was chosen for sampling while in all markets, mangrove loggers were chosen randomly. In addition, log diameters were measured in each market using a wooden foldable tape measure. A post hoc analysis (Newman-Keuls test) was performed in order to detect the common wood class diameter sold in the Douala wood markets. The analysis of the loggers' survey data has shown that large logs of Rhizophora with diameter greater than 40 cm were common in the Douala wood markets and were more closely associated with loggers who used chainsaws. In addition to the general mangroves wood products selling, the analysis on a subsistence level (households' survey) suggests the local population's dependence on mangroves, with multiple uses of Rhizophora racemosa Meyer, R. harrisonii Leechman, Avicennia germinans L. Stearn., Laguncularia racemosa Gaertn. f. and Conocarpus erectus L. timbers for furniture, fences, smoking fish, and fuelwood. Finally, Nypa fruticans (Thunb.) Wurmb. leaves were used as thatching material for house walls and roofs. Our findings revealed that big logs of Rhizophora were commonly sold by the loggers. A majority of loggers (60%) reported that mangrove marketed wood constitute a principal source of income. Most of the villagers (85.83%) often depend on mangroves for subsistence needs and for them there is no substitute for mangrove wood. Therefore, more efforts should be undertaken at the national level to implement conservation, management and sustainable use of these coastal forests.

  14. Commercial activities and subsistence utilization of mangrove forests around the Wouri estuary and the Douala-Edea reserve (Cameroon)

    PubMed Central

    2009-01-01

    Background Worldwide there is growing research interest in the ethnobiology of mangrove forests. Notwithstanding that, little information has been published about ethnobiology of mangrove forests in Cameroon. The aims of this study were a) to analyze the harvesting methods and the local selling of mangrove wood products by loggers in the vicinity of Wouri estuary and b) to investigate the patterns of subsistence uses of mangrove wood products around the Douala-Edea reserve. Methods Semi-structured interviews were conducted with 120 active mangrove loggers in 23 Douala wood markets and 103 households located in three villages (Mbiako, Yoyo I and Yoyo II) close to Douala-Edea reserve. In each of the three densely populated villages, every second household was chosen for sampling while in all markets, mangrove loggers were chosen randomly. In addition, log diameters were measured in each market using a wooden foldable tape measure. A post hoc analysis (Newman-Keuls test) was performed in order to detect the common wood class diameter sold in the Douala wood markets. Results The analysis of the loggers' survey data has shown that large logs of Rhizophora with diameter greater than 40 cm were common in the Douala wood markets and were more closely associated with loggers who used chainsaws. In addition to the general mangroves wood products selling, the analysis on a subsistence level (households' survey) suggests the local population's dependence on mangroves, with multiple uses of Rhizophora racemosa Meyer, R. harrisonii Leechman, Avicennia germinans L. Stearn., Laguncularia racemosa Gaertn. f. and Conocarpus erectus L. timbers for furniture, fences, smoking fish, and fuelwood. Finally, Nypa fruticans (Thunb.) Wurmb. leaves were used as thatching material for house walls and roofs. Conclusion Our findings revealed that big logs of Rhizophora were commonly sold by the loggers. A majority of loggers (60%) reported that mangrove marketed wood constitute a principal source of income. Most of the villagers (85.83%) often depend on mangroves for subsistence needs and for them there is no substitute for mangrove wood. Therefore, more efforts should be undertaken at the national level to implement conservation, management and sustainable use of these coastal forests. PMID:19919680

  15. Instream wood in a steep headwater channel: geomorphic significance of large and small wood

    NASA Astrophysics Data System (ADS)

    Galia, Tomáš; Šilhán, Karel; Ruiz-Villanueva, Virginia; Tichavský, Radek

    2016-04-01

    Besides the well-known significance of large wood (LW), also small woody pieces (SW; here defined as pieces with dimensions at least 0.5 m length and 0.05 m diameter), can play an important role in steep narrow headwaters. We inventoried instream wood in the 0.4 km long Mazák headwater channel, Moravskoslezské Beskydy Mts, Czech Republic (2

  16. Oxidative stress and lung injury induced by short-term exposure to wood smoke in guinea pigs.

    PubMed

    Ramos, Carlos; Pedraza-Chaverri, José; Becerril, C; Cisneros, J; González-Ávila, G; Rivera-Rosales, R; Sommer, B; Medina-Campos, O N; Montaño, M

    2013-11-01

    Oxidative stress and lung injury induced by short-term exposure to wood smoke were evaluated in guinea pigs through cell profile, bronchoalveolar lavage (BAL), conventional histology and immunohistochemistry (4-hydroxynonenal, 3-nitrotyrosine, Mn-superoxide dismutase, heme oxygenase-1); malondialdehyde and 4-hydroxynonenal concentration, Mn-superoxide dismutase, glutathione reductase, glutathione peroxidase, and catalase activities in plasma, lung and BAL. Total cells increased in BAL, and the percentage of macrophages, neutrophils and lymphocytes augmented (72-96 h). Histopathological examination of lung tissues showed mild thickening of membranous bronchiole walls, infiltration of foamy macrophages and polymorphonuclear leukocytes in bronchial, bronchiolar and intraalveolar spaces. Goblet cell hyperplasia was also observed in bronchial and bronchiolar epithelia. Plasma malondialdehyde concentration was increased at all times, while 4-hydroxynonenal was increased only in plasma and BAL after 24 h. Plasma glutathione reductase activity increased at 24 and 72 h, BAL glutathione peroxidase activity decreased at 72 and 96 h, whereas catalase activity increased in plasma at 72 h, and decreased in BAL at 24 h. Immunostaining intensity to 4-hydroxynonenal, 3-nitrotyrosine, Mn-superoxide dismutase and heme oxygenase-1 was enhanced mainly in macrophages, bronchial/bronchiolar epithelial cells and type II pneumocytes after 72-96 h of wood smoke exposure. Overall, short-term exposure to wood smoke induces alterations in oxidative/antioxidant state in lung and airway injury, similar to those observed in humans with domestic exposure.

  17. Aspidosperma (Apocynaceae) plant cytotoxicity and activity towards malaria parasites. Part I: Aspidosperma nitidum (Benth) used as a remedy to treat fever and malaria in the Amazon.

    PubMed

    Coutinho, Julia Penna; Aguiar, Anna Caroline Campos; dos Santos, Pierre Alexandre; Lima, Joaquim Corsino; Rocha, Maria Gabrielle Lima; Zani, Carlos Leomar; Alves, Tânia Maria Almeida; Santana, Antônio Euzébio Goulart; Pereira, Maria de Meneses; Krettli, Antoniana Ursine

    2013-12-01

    Infusions of Aspidosperma nitidum (Apocynaceae) wood bark are used to treat fever and malaria in the Amazon Region. Several species of this family are known to possess indole alkaloids and other classes of secondary metabolites, whereas terpenoids, an inositol and the indole alkaloids harmane-3 acid and braznitidumine have been described in A. nitidum . In the present study, extracts from the wood bark, leaves and branches of this species were prepared for assays against malaria parasites and cytotoxicity testing using human hepatoma and normal monkey kidney cells. The wood bark extracts were active against Plasmodium falciparum and showed a low cytotoxicity in vitro, whereas the leaf and branch extracts and the pure alkaloid braznitidumine were inactive. A crude methanol extract was subjected to acid-base fractionation aimed at obtaining alkaloid-rich fractions, which were active at low concentrations against P. falciparum and in mice infected with and sensitive Plasmodium berghei parasites. Our data validate the antimalarial usefulness of A. nitidum wood bark, a remedy that can most likely help to control malaria. However, the molecules responsible for this antimalarial activity have not yet been identified. Considering their high selectivity index, the alkaloid-rich fractions from the plant bark might be useful in the development of new antimalarials.

  18. In vivo biochemical and gene expression analyses of the antioxidant activities and hypocholesterolaemic properties of Tamarindus indica fruit pulp extract.

    PubMed

    Lim, Chor Yin; Mat Junit, Sarni; Abdulla, Mahmood Ameen; Abdul Aziz, Azlina

    2013-01-01

    Tamarindus indica (T. indica) is a medicinal plant with many biological activities including anti-diabetic, hypolipidaemic and anti-bacterial activities. A recent study demonstrated the hypolipidaemic effect of T. indica fruit pulp in hamsters. However, the biochemical and molecular mechanisms responsible for these effects have not been fully elucidated. Hence, the aims of this study were to evaluate the antioxidant activities and potential hypocholesterolaemic properties of T. indica, using in vitro and in vivo approaches. The in vitro study demonstrated that T. indica fruit pulp had significant amount of phenolic (244.9 ± 10.1 mg GAE/extract) and flavonoid (93.9 ± 2.6 mg RE/g extract) content and possessed antioxidant activities. In the in vivo study, hamsters fed with high-cholesterol diet for ten weeks showed elevated serum triglyceride, total cholesterol, HDL-C and LDL-C levels. Administration of T. indica fruit pulp to hypercholesterolaemic hamsters significantly lowered serum triglyceride, total cholesterol and LDL-C levels but had no effect on the HDL-C level. The lipid-lowering effect was accompanied with significant increase in the expression of Apo A1, Abcg5 and LDL receptor genes and significant decrease in the expression of HMG-CoA reductase and Mtp genes. Administration of T. indica fruit pulp to hypercholesterolaemic hamsters also protected against oxidative damage by increasing hepatic antioxidant enzymes, antioxidant activities and preventing hepatic lipid peroxidation. It is postulated that tamarind fruit pulp exerts its hypocholesterolaemic effect by increasing cholesterol efflux, enhancing LDL-C uptake and clearance, suppressing triglyceride accumulation and inhibiting cholesterol biosynthesis. T. indica fruit pulp has potential antioxidative effects and is potentially protective against diet-induced hypercholesterolaemia.

  19. In Vivo Biochemical and Gene Expression Analyses of the Antioxidant Activities and Hypocholesterolaemic Properties of Tamarindus indica Fruit Pulp Extract

    PubMed Central

    Lim, Chor Yin; Mat Junit, Sarni; Abdulla, Mahmood Ameen; Abdul Aziz, Azlina

    2013-01-01

    Background Tamarindus indica (T. indica) is a medicinal plant with many biological activities including anti-diabetic, hypolipidaemic and anti-bacterial activities. A recent study demonstrated the hypolipidaemic effect of T. indica fruit pulp in hamsters. However, the biochemical and molecular mechanisms responsible for these effects have not been fully elucidated. Hence, the aims of this study were to evaluate the antioxidant activities and potential hypocholesterolaemic properties of T. indica, using in vitro and in vivo approaches. Methodology/Principal Findings The in vitro study demonstrated that T. indica fruit pulp had significant amount of phenolic (244.9±10.1 mg GAE/extract) and flavonoid (93.9±2.6 mg RE/g extract) content and possessed antioxidant activities. In the in vivo study, hamsters fed with high-cholesterol diet for ten weeks showed elevated serum triglyceride, total cholesterol, HDL-C and LDL-C levels. Administration of T. indica fruit pulp to hypercholesterolaemic hamsters significantly lowered serum triglyceride, total cholesterol and LDL-C levels but had no effect on the HDL-C level. The lipid-lowering effect was accompanied with significant increase in the expression of Apo A1, Abcg5 and LDL receptor genes and significant decrease in the expression of HMG-CoA reductase and Mtp genes. Administration of T. indica fruit pulp to hypercholesterolaemic hamsters also protected against oxidative damage by increasing hepatic antioxidant enzymes, antioxidant activities and preventing hepatic lipid peroxidation. Conclusion/Significance It is postulated that tamarind fruit pulp exerts its hypocholesterolaemic effect by increasing cholesterol efflux, enhancing LDL-C uptake and clearance, suppressing triglyceride accumulation and inhibiting cholesterol biosynthesis. T. indica fruit pulp has potential antioxidative effects and is potentially protective against diet-induced hypercholesterolaemia. PMID:23894592

  20. Physiological Effects of Touching Wood

    PubMed Central

    2017-01-01

    This study aimed to clarify the physiological effects of touching wood with the palm, in comparison with touching other materials on brain activity and autonomic nervous activity. Eighteen female university students (mean age, 21.7  ±  1.6 years) participated in the study. As an indicator of brain activity, oxyhemoglobin (oxy-Hb) concentrations were measured in the left/right prefrontal cortex using near-infrared time-resolved spectroscopy. Heart rate variability (HRV) was used as an indicator of autonomic nervous activity. The high-frequency (HF) component of HRV, which reflected parasympathetic nervous activity, and the low-frequency (LF)/HF ratio, which reflected sympathetic nervous activity, were measured. Plates of uncoated white oak, marble, tile, and stainless steel were used as tactile stimuli. After sitting at rest with their eyes closed, participants touched the materials for 90 s. As a result, tactile stimulation with white oak significantly (1) decreased the oxy-Hb concentration in the left/right prefrontal cortex relative to marble, tile, and stainless steel and (2) increased ln(HF)-reflected parasympathetic nervous activity relative to marble and stainless steel. In conclusion, our study revealed that touching wood with the palm calms prefrontal cortex activity and induces parasympathetic nervous activity more than other materials, thereby inducing physiological relaxation. PMID:28718814

  1. Antioxidant Effects of Four Heartwood Extractives on Midgut Enzyme Activity in Heterotermes indicola (Blattodea: Rhinotermitidae).

    PubMed

    Hassan, Babar; Ahmed, Sohail; Kirker, Grant; Mankowski, Mark E; Misbah-Ul-Haq, Muhammad

    2018-06-06

    Heterotermes indicola (Wasmann) (Blattodea: Rhinotermitidae) is a species of subterranean termite that is a destructive pest of wood and wood products in Pakistan. This study evaluated the antioxidant and antienzyme potential of heartwood extractives against H. indicola. Heartwood extractives of four durable wood species, Tectona grandis (L.f), Dalbergia sissoo (Roxb.), Cedrus deodara (Roxb.), and Pinus roxburghii (Sarg.) were removed from wood shavings via soxhlet extraction with an ethanol:toluene solvent system. The antioxidant potential of the extractive compounds was determined using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging test. Results showed maximum antioxidant activity for extractives of D. sissoo. D. sissoo had the lowest IC50 (the concentration where 50% inhibition of the DPPH radical is obtained) at 28.83 µg/ml among the heartwood extractives evaluated. This antioxidant activity, however, was not concentration dependent as was observed in the other heartwood extractives tested. At the maximum test concentration, T. grandis showed the highest percent inhibition at 89.7%, but this inhibition was lower compared to the positive control antioxidant compounds butylated hydroxytoluene and quercetin. When termites were fed filter paper treated with IC50s of the extractives and control compounds, glutathione S-transferase activity in the guts of H. indicola workers was significantly reduced by T. grandis and D. sissoo extractives. Similarly, esterase activity was reduced more by P. roxburghii extractives compared to control antioxidant treatments and other tested extractives. However, none of the extractives examined significantly reduced the activity of catalase enzymes in H. indicola compared to treatments with the antioxidant control compounds.

  2. Local indigenous fruit-derived juices as alternate source of acidity regulators.

    PubMed

    D'souza, Cassandra; Fernandes, Rosaline; Kudale, Subhash; Naik, Azza Silotry

    2018-03-01

    Acidity regulators are additives that alter and control food acidity. The objective of this study was to explore local indigenous fruits as sources of natural acidity regulators. Juices extracted from Garcinia indica (kokum), Embilica officinalis (amla) and Tamarindus indica (tamarind) were used as acidulants for media such as coconut milk and bottle gourd juice. The buffering capacity β, acid composition, antioxidant activity and shelf-life study of the acidified media were estimated. Potentiometric titration showed G. indica to possess the highest buffering capacity in both ranges. High-performance liquid chromatography analysis showed T. indica contained a high level of tartaric acid (4.84 ± 0.01 mg g -1 ), while G. indica had citric acid (22.37 ± 0.84 mg g -1 ) and E. officinalis had citric acid (2.75 ± 0.02 mg g -1 ) along with ascorbic acid (2.68 ± 0.01 mg g -1 ). 1,1-Diphenyl-2-picrylhydrazyl scavenging activity was high for E. officinalis (91.24 ± 0.66%) and T. indica (90.93 ± 0.817%) and relatively lower for G. indica (34.61 ± 3.66%). The shelf-life study showed total plate count to be within the prescribed limits up to a week, in accordance with safety regulations. This investigation confirmed the suitability of indigenous fruit juices as alternatives to existing acidity regulators. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  3. Xylanases, nucleic acids encoding them and methods for making and using them

    DOEpatents

    Gray, Kevin A; Dirmeier, Reinhard

    2013-07-16

    The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal .beta.-1,4-xylosidic linkages or endo-.beta.-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.

  4. Xylanases, nucleic acids encoding them and methods for making and using them

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Gray, Kevin A.; Dirmeier, Richard

    The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal .beta.-1,4-xylosidic linkages or endo-.beta.-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanasesmore » have increased activity and stability at increased pH and temperature.« less

  5. Interannual kinetics (2010-2013) of large wood in a river corridor exposed to a 50-year flood event and fluvial ice dynamics

    NASA Astrophysics Data System (ADS)

    Boivin, Maxime; Buffin-Bélanger, Thomas; Piégay, Hervé

    2017-02-01

    Semi-alluvial rivers of the Gaspé Peninsula, Québec, are prone to produce and transport vast quantities of large wood (LW). The high rate of lateral erosion owing to high energy flows and noncohesive banks is the main process leading to the recruitment of large wood, which in turn initiates complex patterns of wood accumulation and reentrainment within the active channel. The delta of the Saint-Jean River (SJR) has accumulated large annual wood fluxes since 1960 that culminated in a wood raft of > 3-km in length in 2014. To document the kinetics of large wood on the main channel of SJR, four annual surveys were carried out from 2010 to 2013 to locate and describe > 1000 large wood jams (LWJ) and 2000 large wood individuals (LWI) along a 60-km river section. Airborne and ground photo/video images were used to estimate the wood volume introduced by lateral erosion and to identify local geomorphic conditions that control wood mobility and deposits. Video camera analysis allowed the examination of transport rates from three hydrometeorological events for specific river sections. Results indicate that the volume of LW recruited between 2010 and 2013 represents 57% of the total LW production over the 2004-2013 period. Volumes of wood deposited along the 60-km section were four times higher in 2013 than in 2010. Increases in wood amount occurred mainly in upper alluvial sections of the river, whereas decreases were observed in the semi-alluvial middle sections. Observations suggest that the 50-year flood event of 2010 produced large amounts of LW that were only partly exported out of the basin so that a significant amount was still available for subsequent floods. Large wood storage continued after this flood until a similar flood or an ice-breakup event could remobilise these LW accumulations into the river corridor. Ice-jam floods transport large amounts of wood during events with fairly low flow but do not contribute significantly to recruitment rates (ca. 10 to 30% early). It is fairly probable that the wood export peak observed in 2012 at the river mouth, where no flood occurred and which is similar to the 1-in 10-year flood of 2010, is mainly linked to such ice-break events that occurred in March 2012.

  6. Development of a pathway model to assess the exposure of European pine trees to pine wood nematode via the trade of wood.

    PubMed

    Douma, J C; van der Werf, W; Hemerik, L; Magnusson, C; Robinet, C

    2017-04-01

    Pine wood nematode (PWN), Bursaphelenchus xylophilus, is a threat for pine species (Pinus spp.) throughout the world. The nematode is native to North America, and invaded Japan, China, Korea, and Taiwan, and more recently Portugal and Spain. PWN enters new areas through trade in wood products. Once established, eradication is not practically feasible. Therefore, preventing entry of PWN into new areas is crucial. Entry risk analysis can assist in targeting management to reduce the probability of entry. Assessing the entry of PWN is challenging due to the complexity of the wood trade and the wood processing chain. In this paper, we develop a pathway model that describes the wood trade and wood processing chain to determine the structure of the entry process. We consider entry of PWN through imported coniferous wood from China, a possible origin of Portuguese populations, to Europe. We show that exposure increased over years due to an increase in imports of sawn wood. From 2000 to 2012, Europe received an estimated 84 PWN propagules from China, 88% of which arose from imported sawn wood and 12% from round wood. The region in Portugal where the PWN was first reported is among those with the highest PWN transfer per unit of imported wood due to a high host cover and vector activity. An estimated 62% of PWN is expected to enter in countries where PWN is not expected to cause the wilt of pine trees because of low summer temperatures (e.g., Belgium, Sweden, Norway). In these countries, PWN is not easily detected, and such countries can thus serve as potential reservoirs of PWN. The model identifies ports and regions with high exposure, which helps targeting monitoring and surveillance, even in areas where wilt disease is not expected to occur. In addition, we show that exposure is most efficiently reduced by additional treatments in the country of origin, and/or import wood from PWN-free zones. Pathway modelling assists plant health managers in analyzing risks along the pathway and planning measures for enhancing biosecurity. © 2016 by the Ecological Society of America.

  7. Whole-cell biosensor of cellobiose and application to wood decay detection.

    PubMed

    Toussaint, Maxime; Bontemps, Cyril; Besserer, Arnaud; Hotel, Laurence; Gérardin, Philippe; Leblond, Pierre

    2016-12-10

    Fungal biodegradation of wood is one of the main threats regarding its use as a material. So far, the detection of this decaying process is empirically assessed by loss of mass, when the fungal attack is advanced and woody structure already damaged. Being able to detect fungal attack on wood in earlier steps is thus of special interest for the wood economy. In this aim, we designed here a new diagnostic tool for wood degradation detection based on the bacterial whole-cell biosensor technology. It was designed in diverting the soil bacteria Streptomyces CebR sensor system devoted to cellobiose detection, a cellulolytic degradation by-product emitted by lignolytic fungi since the onset of wood decaying process. The conserved regulation scheme of the CebR system among Streptomyces allowed constructing a molecular tool easily transferable in different strains or species and enabling the screen for optimal host strains for cellobiose detection. Assays are performed in microplates using one-day culture lysates. Diagnostic is performed within one hour by a spectrophotometric measuring of the cathecol deshydrogenase activity. The selected biosensor was able to detect specifically cellobiose at concentrations similar to those measured in decaying wood and in a spruce leachate attacked by a lignolytic fungus, indicating a high potential of applicability to detect ongoing wood decay process. Copyright © 2016 Elsevier B.V. All rights reserved.

  8. Micronized Copper Wood Preservatives: Efficacy of Ion, Nano, and Bulk Copper against the Brown Rot Fungus Rhodonia placenta

    PubMed Central

    Civardi, Chiara; Schubert, Mark; Fey, Angelika; Wick, Peter; Schwarze, Francis W. M. R.

    2015-01-01

    Recently introduced micronized copper (MC) formulations, consisting of a nanosized fraction of basic copper (Cu) carbonate (CuCO3·Cu(OH)2) nanoparticles (NPs), were introduced to the market for wood protection. Cu NPs may presumably be more effective against wood-destroying fungi than bulk or ionic Cu compounds. In particular, Cu- tolerant wood-destroying fungi may not recognize NPs, which may penetrate into fungal cell walls and membranes and exert their impact. The objective of this study was to assess if MC wood preservative formulations have a superior efficacy against Cu-tolerant wood-destroying fungi due to nano effects than conventional Cu biocides. After screening a range of wood-destroying fungi for their resistance to Cu, we investigated fungal growth of the Cu-tolerant fungus Rhodonia placenta in solid and liquid media and on wood treated with MC azole (MCA). In liquid cultures we evaluated the fungal response to ion, nano and bulk Cu distinguishing the ionic and particle effects by means of the Cu2+ chelator ammonium tetrathiomolybdate (TTM) and measuring fungal biomass, oxalic acid production and laccase activity of R. placenta. Our results do not support the presence of particular nano effects of MCA against R. placenta that would account for an increased antifungal efficacy, but provide evidence that attribute the main effectiveness of MCA to azoles. PMID:26554706

  9. Micronized Copper Wood Preservatives: Efficacy of Ion, Nano, and Bulk Copper against the Brown Rot Fungus Rhodonia placenta.

    PubMed

    Civardi, Chiara; Schubert, Mark; Fey, Angelika; Wick, Peter; Schwarze, Francis W M R

    2015-01-01

    Recently introduced micronized copper (MC) formulations, consisting of a nanosized fraction of basic copper (Cu) carbonate (CuCO3·Cu(OH)2) nanoparticles (NPs), were introduced to the market for wood protection. Cu NPs may presumably be more effective against wood-destroying fungi than bulk or ionic Cu compounds. In particular, Cu- tolerant wood-destroying fungi may not recognize NPs, which may penetrate into fungal cell walls and membranes and exert their impact. The objective of this study was to assess if MC wood preservative formulations have a superior efficacy against Cu-tolerant wood-destroying fungi due to nano effects than conventional Cu biocides. After screening a range of wood-destroying fungi for their resistance to Cu, we investigated fungal growth of the Cu-tolerant fungus Rhodonia placenta in solid and liquid media and on wood treated with MC azole (MCA). In liquid cultures we evaluated the fungal response to ion, nano and bulk Cu distinguishing the ionic and particle effects by means of the Cu2+ chelator ammonium tetrathiomolybdate (TTM) and measuring fungal biomass, oxalic acid production and laccase activity of R. placenta. Our results do not support the presence of particular nano effects of MCA against R. placenta that would account for an increased antifungal efficacy, but provide evidence that attribute the main effectiveness of MCA to azoles.

  10. Investigating the Geomorphic and Ecologic Functions of Wood in Relationship to Habitat Type and Salmonid Redds on a Regulated California River

    NASA Astrophysics Data System (ADS)

    Senter, A. E.; Pasternack, G. B.

    2006-12-01

    Most river rehabilitation projects incorporate little to no wood in current designs, and those that do have little science to guide them. The overall goal of this research is to investigate the role of wood in a regulated, mid- sized (where river width is greater than most tree heights), Mediterranean-climate (where smaller, softer-wood trees dominate the landscape) river in order to provide a scientific foundation for the potential use of wood in rehabilitation projects within such systems. Wood structures in the active salmonid spawning reach of the Lower Mokelumne River in Central California were measured, mapped, and described during summer and fall 2006. Digital photos and GPS coordinates were used to establish wood location within the stream channel. Structural morphology was determined by measuring physical properties such as individual diameter and length, orientation to stream flow, and jam dimensions. In addition, qualitative attributes were recorded such as decay class and leaf, limb, bark, and root characteristics. A GIS wood layer will be created and added to a database of existing Mokelumne River GIS layers containing salmonid redd (salmon egg nests) densities, hydraulic conditions associated with individual redds, and sub-reach habitat types. An analysis of wood properties, redd locations and conditions, and habitat types will be used to develop a conceptual model of wood dynamics in relation to salmonid habitat on the Lower Mokelumne River. The primary products of this study will be (1) a scientific conceptual model of the role of wood in regulated gravel reaches of mid-size rivers in Mediterranean California and (2) a decision-making framework that will enable river managers to include scientifically based wood structures into rehabilitation designs, thereby enhancing spawning habitat, stream complexity, and biological diversity. These tools will be developed in collaboration with East Bay Municipal Utilities District to aid in the continuing rehabilitation of the Lower Mokelumne River.

  11. Selection and validation of enzymatic activities as functional markers in wood biotechnology and fungal ecology.

    PubMed

    Mathieu, Yann; Gelhaye, Eric; Dumarçay, Stéphane; Gérardin, Philippe; Harvengt, Luc; Buée, Marc

    2013-02-15

    The dead wood and forest soils are sources of diversity and under-explored fungal strains with biotechnological potential, which require to be studied. Numerous enzymatic tests have been proposed to investigate the functional potential of the soil microbial communities or to test the functional abilities of fungal strains. Nevertheless, the diversity of these functional markers and their relevance in environmental studies or biotechnological screening does still have not been demonstrated. In this work, we assessed ten different extracellular enzymatic activities involved in the wood decaying process including β-etherase that specifically cleaves the β-aryl ether linkages in the lignin polymer. For this purpose, a collection of 26 fungal strains, distributed within three ecological groups (white, brown and soft rot fungi), has been used. Among the ten potential functional markers, the combinatorial use of only six of them allowed separation between the group of white and soft rot fungi from the brown rot fungi. Moreover, our results suggest that extracellular β-etherase is a rare and dispensable activity among the wood decay fungi. Finally, we propose that this set of markers could be useful for the analysis of fungal communities in functional and environmental studies, and for the selection of strains with biotechnological interests. Copyright © 2012 Elsevier B.V. All rights reserved.

  12. Executive summary.

    Treesearch

    David B. McKeever

    1997-01-01

    The repair and remodeling of residential units in the existing housing stock is an important market for wood products. Included are many, and varied repair and remodeling activities and projects, some of which require substantial amounts of wood products, some of which do not. Currently, about 24 percent of all lumber, 23 percent of all structural panels, 15 percent of...

  13. Species comparison of the physical properties of loblolly and slash pine wood and bark

    Treesearch

    Thomas L. Eberhardt; Joseph Dahlen; Laurence Schimleck

    2017-01-01

    Composition of the southern pine forest is now predominated by two species, loblolly pine (Pinus taeda L.) and slash pine (Pinus elliottii Engelm.), owing to fire suppression activities, natural regeneration on abandoned agricultural lands, and extensive planting. Comparison of the wood and bark physical properties of these...

  14. Building Environmental Awareness: Teaching Campers To Minimize Their Impact.

    ERIC Educational Resources Information Center

    Henchey, Kathy; Carvajal, Michelle

    2000-01-01

    Three activities are described to teach campers the importance of minimizing their impact on the environment. Soil Buggy Bingo shows fire's impact on living things in the soil; Food Web demonstrates how everything in an ecosystem is interconnected; and Camouflaging--Field versus Woods shows the difference between wooded and cleared areas in the…

  15. Sondheim's "Into the Woods". ArtsEdge Curricula, Lessons and Activities.

    ERIC Educational Resources Information Center

    Bauernschub, Mary Beth

    This curriculum unit introduces intermediate grade and middle school students to the work of Stephen Sondheim, one of the most talented composers of the contemporary American musical theater, and teaches them about the process of writing an original musical. The unit notes that in his musical "Into the Woods" Sondheim incorporates (and…

  16. Identifying Effective Pedagogical Approaches for Online Workplace Training: A Case Study of the South African Wood Products Manufacturing Sector

    ERIC Educational Resources Information Center

    Macdonald, Ian S.; Bullen, Mark; Kozak, R. A.

    2007-01-01

    This study investigated appropriate pedagogical techniques for workplace e-learning programs in the South African wood products (furniture) manufacturing sector. The study found that learners responded favourably to constructivist teaching approaches, such as asynchronous discussions, open-ended task-based activities, and assignments incorporating…

  17. Helping your woodland adapt to climate change

    Treesearch

    Tracey Saxby; Marcus Griswold; Caroline Wicks

    2013-01-01

    Your woods are always changing and adapting as they grow and mature, or regrow after agricultural abandonment, natural disturbances, or harvesting activities. Events like storms, droughts, insect and disease outbreaks, or other stressors can damage trees or slow their growth. A changing climate may make your woods more susceptible to the problems these events can cause...

  18. Bacteroidales ectosymbionts of gut flagellates shape the nitrogen-fixing community in dry-wood termites

    PubMed Central

    Desai, Mahesh S; Brune, Andreas

    2012-01-01

    Although it is well documented that the lack of nitrogen in the diet of wood-feeding termites is compensated by the nitrogen-fixing capacity of their gut microbiota, the bacteria responsible for this activity are largely unknown. Here, we analyzed the diversity and expression of nitrogenase genes (homologs of nifH) in four species of dry-wood termites (Kalotermitidae), which thrive on a particularly nitrogen-poor resource. Although each species harbored a highly diverse suite of termite-specific homologs in their microliter-sized hindgut, only a core set related to nifH genes of Treponema and Azoarcus spp., ‘Azobacteroides pseudotrichonymphae', the first member of the Bacteroidales identified as a diazotroph, and termite-gut-specific anfH genes of hitherto unknown origin were preferentially expressed. Transcription patterns corroborated that the populations of active diazotrophs differ fundamentally between termite genera. Capillary-picked suspensions of the flagellates Devescovina arta and Snyderella tabogae revealed that their bacterial ectosymbionts each possess two paralogs of nifH, which apparently have been acquired consecutively during evolution of Bacteroidales, but only one of them (anfH) is actively expressed. Transcription patterns correlated neither with the molybdenum content of the diet nor with intestinal hydrogen concentrations, measured with microsensors. We propose that the nitrogen-fixing community in different dry-wood termites is shaped by the symbionts of their specific flagellate populations. Our findings suggest that the diazotrophic nature of ‘Armantifilum devescovinae' has an important role in the nitrogen metabolism of dry-wood termites and is the driving force of co-evolution with its flagellate host. PMID:22189498

  19. Fabrication of a Nano-ZnO/Polyethylene/Wood-Fiber Composite with Enhanced Microwave Absorption and Photocatalytic Activity via a Facile Hot-Press Method

    PubMed Central

    Dang, Baokang; Chen, Yipeng; Shen, Xiaoping; Chen, Bo; Sun, Qingfeng; Jin, Chunde

    2017-01-01

    A polyethylene/wood-fiber composite loaded with nano-ZnO was prepared by a facile hot-press method and was used for the photocatalytic degradation of organic compounds as well as for microwave absorption. ZnO nanoparticles with an average size of 29 nm and polyethylene (PE) powders were dispersed on the wood fibers’ surface through a viscous cationic polyacrylamide (CPAM) solution. The reflection loss (RL) value of the resulting composite was −21 dB, with a thickness of 3.5 mm in the frequency of 17.17 GHz. The PE/ZnO/wood-fiber (PZW) composite exhibited superior photocatalytic activity (84% methyl orange degradation within 300 min) under UV light irradiation. ZnO nanoparticels (NPs) increased the storage modulus of the PZW composite, and the damping factor was transferred to the higher temperature region. The PZW composite exhibited the maximum flexural strength of 58 MPa and a modulus of elasticity (MOE) of 9625 MPa. Meanwhile, it also displayed dimensional stability (thickness swelling value of 9%). PMID:29099777

  20. Characteristic Assessment of Diesel-degrading Bacteria Immobilized on Natural Organic Carriers in Marine Environment: the Degradation Activity and Nutrient.

    PubMed

    Xue, Jianliang; Wu, Yanan; Liu, Zhixiu; Li, Menglu; Sun, Xiyu; Wang, Huajun; Liu, Bing

    2017-08-17

    Oil spill has led to severe environmental and ecological problems. Due to the harsh environmental conditions, the bioremediation technology is not successfully used to remedy the oil spill in marine environment. In this study, immobilization technology was used to immobilize bacteria on natural organic carriers (i.e., wood chips and maize straw). The higher surface area of in wood chips leads to larger biomass density (0.0242 gVSS/g) than that of maize straw of 0.0097 gVSS/g carrier. Compared with biodegradation efficiency of free bacteria (44.79%), the immobilized bacteria on wood chips and maize straw reached to 73.39% and 52.28%, respectively. The high biological activity of the immobilized bacteria can be also explained by nutrients, such as TN (total nitrogen) and TP (total phosphorus), released from wood chips and maize straw, which was 8.83 mg/g and 5.53 mg/g, 0.0624 mg/g and 0.0099 mg/g, respectively.

  1. Effect of wood smoke exposure on vascular function and thrombus formation in healthy fire fighters.

    PubMed

    Hunter, Amanda L; Unosson, Jon; Bosson, Jenny A; Langrish, Jeremy P; Pourazar, Jamshid; Raftis, Jennifer B; Miller, Mark R; Lucking, Andrew J; Boman, Christoffer; Nyström, Robin; Donaldson, Kenneth; Flapan, Andrew D; Shah, Anoop S V; Pung, Louis; Sadiktsis, Ioannis; Masala, Silvia; Westerholm, Roger; Sandström, Thomas; Blomberg, Anders; Newby, David E; Mills, Nicholas L

    2014-12-09

    Myocardial infarction is the leading cause of death in fire fighters and has been linked with exposure to air pollution and fire suppression duties. We therefore investigated the effects of wood smoke exposure on vascular vasomotor and fibrinolytic function, and thrombus formation in healthy fire fighters. In a double-blind randomized cross-over study, 16 healthy male fire fighters were exposed to wood smoke (~1 mg/m³ particulate matter concentration) or filtered air for one hour during intermittent exercise. Arterial pressure and stiffness were measured before and immediately after exposure, and forearm blood flow was measured during intra-brachial infusion of endothelium-dependent and -independent vasodilators 4-6 hours after exposure. Thrombus formation was assessed using the ex vivo Badimon chamber at 2 hours, and platelet activation was measured using flow cytometry for up to 24 hours after the exposure. Compared to filtered air, exposure to wood smoke increased blood carboxyhaemoglobin concentrations (1.3% versus 0.8%; P < 0.001), but had no effect on arterial pressure, augmentation index or pulse wave velocity (P > 0.05 for all). Whilst there was a dose-dependent increase in forearm blood flow with each vasodilator (P < 0.01 for all), there were no differences in blood flow responses to acetylcholine, sodium nitroprusside or verapamil between exposures (P > 0.05 for all). Following exposure to wood smoke, vasodilatation to bradykinin increased (P = 0.003), but there was no effect on bradykinin-induced tissue-plasminogen activator release, thrombus area or markers of platelet activation (P > 0.05 for all). Wood smoke exposure does not impair vascular vasomotor or fibrinolytic function, or increase thrombus formation in fire fighters. Acute cardiovascular events following fire suppression may be precipitated by exposure to other air pollutants or through other mechanisms, such as strenuous physical exertion and dehydration.

  2. Patterns of functional enzyme activity in fungus farming ambrosia beetles.

    PubMed

    De Fine Licht, Henrik H; Biedermann, Peter H W

    2012-06-06

    In wood-dwelling fungus-farming weevils, the so-called ambrosia beetles (Curculionidae: Scolytinae and Platypodinae), wood in the excavated tunnels is used as a medium for cultivating fungi by the combined action of digging larvae (which create more space for the fungi to grow) and of adults sowing and pruning the fungus. The beetles are obligately dependent on the fungus that provides essential vitamins, amino acids and sterols. However, to what extent microbial enzymes support fungus farming in ambrosia beetles is unknown. Here we measure (i) 13 plant cell-wall degrading enzymes in the fungus garden microbial consortium of the ambrosia beetle Xyleborinus saxesenii, including its primary fungal symbionts, in three compartments of laboratory maintained nests, at different time points after gallery foundation and (ii) four specific enzymes that may be either insect or microbially derived in X. saxesenii adult and larval individuals. We discovered that the activity of cellulases in ambrosia fungus gardens is relatively small compared to the activities of other cellulolytic enzymes. Enzyme activity in all compartments of the garden was mainly directed towards hemicellulose carbohydrates such as xylan, glucomannan and callose. Hemicellulolytic enzyme activity within the brood chamber increased with gallery age, whereas irrespective of the age of the gallery, the highest overall enzyme activity were detected in the gallery dump material expelled by the beetles. Interestingly endo-β-1,3(4)-glucanase activity capable of callose degradation was identified in whole-body extracts of both larvae and adult X. saxesenii, whereas endo-β-1,4-xylanase activity was exclusively detected in larvae. Similar to closely related fungi associated with bark beetles in phloem, the microbial symbionts of ambrosia beetles hardly degrade cellulose. Instead, their enzyme activity is directed mainly towards comparatively more easily accessible hemicellulose components of the ray-parenchyma cells in the wood xylem. Furthermore, the detection of xylanolytic enzymes exclusively in larvae (which feed on fungus colonized wood) and not in adults (which feed only on fungi) indicates that only larvae (pre-) digest plant cell wall structures. This implies that in X. saxesenii and likely also in many other ambrosia beetles, adults and larvae do not compete for the same food within their nests - in contrast, larvae increase colony fitness by facilitating enzymatic wood degradation and fungus cultivation.

  3. Health assessment for Texarkana Wood Preserving Company, Texarkana, Bowie County, Texas, Region 6. CERCLIS No. TXD008056152. Addendum. Final report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    The Texarkana Wood Preserving Company (TWPC) is a National Priorities List site located in northeastern Texas, at the southern extremity of the City of Texarkana in Bowie County. The TWPC site has been used for various lumber-related activities since the early 1900s and for creosoting operations since the early 1950s. Contaminated soils, groundwater, surface water, and surface water sediments have been detected on and off of the TWPC site. The primary contaminants of concern are polycyclic aromatic hydrocarbons, pentachlorophenol, chlorinated dibenzodioxins and chlorinated dibenzofurans. The population at greatest risk of exposure to these contaminants are on-site workers engaged in remedialmore » activities. There are currently no residences or businesses located immediately adjacent to the site and no documentation that contaminated groundwater is being used for potable purposes. The Texarkana Wood Preserving Company site has been evaluated by the Agency for Toxic Substances and Disease Registry (ATSDR) Health Activities Recommendation Panel (HARP) for appropriate follow-up with respect to health activities.« less

  4. Temporal and spatial constraints on community assembly during microbial colonization of wood in seawater.

    PubMed

    Kalenitchenko, Dimitri; Fagervold, Sonja K; Pruski, Audrey M; Vétion, Gilles; Yücel, Mustafa; Le Bris, Nadine; Galand, Pierre E

    2015-12-01

    Wood falls on the ocean floor form chemosynthetic ecosystems that remain poorly studied compared with features such as hydrothermal vents or whale falls. In particular, the microbes forming the base of this unique ecosystem are not well characterized and the ecology of communities is not known. Here we use wood as a model to study microorganisms that establish and maintain a chemosynthetic ecosystem. We conducted both aquaria and in situ deep-sea experiments to test how different environmental constraints structure the assembly of bacterial, archaeal and fungal communities. We also measured changes in wood lipid concentrations and monitored sulfide production as a way to detect potential microbial activity. We show that wood falls are dynamic ecosystems with high spatial and temporal community turnover, and that the patterns of microbial colonization change depending on the scale of observation. The most illustrative example was the difference observed between pine and oak wood community dynamics. In pine, communities changed spatially, with strong differences in community composition between wood microhabitats, whereas in oak, communities changed more significantly with time of incubation. Changes in community assembly were reflected by changes in phylogenetic diversity that could be interpreted as shifts between assemblies ruled by species sorting to assemblies structured by competitive exclusion. These ecological interactions followed the dynamics of the potential microbial metabolisms accompanying wood degradation in the sea. Our work showed that wood is a good model for creating and manipulating chemosynthetic ecosystems in the laboratory, and attracting not only typical chemosynthetic microbes but also emblematic macrofaunal species.

  5. Antimicrobial activity of some Pacific Northwest woods against anaerobic bacteria and yeast.

    PubMed

    Johnston, W H; Karchesy, J J; Constantine, G H; Craig, A M

    2001-11-01

    Extracts of woods commonly used for animal bedding were tested for antimicrobial activity. Essential oils from Alaska cedar (Chamaecyparis nootkatensis), western juniper (Juniperus occidentalis) and old growth Douglas fir (Pseudotsuga menziesii) as well as methanol extracts of wood from these trees plus western red cedar (Thuja plicata) and ponderosa pine (Pinus ponderosa) were tested for antimicrobial activity against anaerobic bacteria and yeast. The test microbes included Fusobacterium necrophorum, Clostridium perfringens, Actinomyces bovis and Candida albicans which are common to foot diseases and other infections in animals. The essential oils and methanol extracts were tested using a standardized broth assay. Only extracts of Alaska cedar and western juniper showed significant antimicrobial activity against each of the microbes tested. The essential oil of Douglas fir did show antimicrobial activity against A. bovis at the concentrations tested. The methanol extracts of the heartwood of Douglas fir and the sapwood of ponderosa pine showed no antimicrobial activity. The major chemical components of western juniper (cedrol and alpha- and beta-cedrene) and Alaska cedar (nootkatin) were also tested. In western juniper, alpha- and beta-cedrene were found to be active components. Nootkatin showed activity only against C. albicans. The inhibitory activity in Alaska cedar oil was high enough to justify further efforts to define the other chemical components responsible for the antimicrobial activity. Copyright 2001 John Wiley & Sons, Ltd.

  6. Grapevine Botryosphaeria dieback fungi have specific aggressiveness factor repertory involved in wood decay and stilbene metabolization.

    PubMed

    Stempien, Elodie; Goddard, Mary-Lorène; Wilhelm, Kim; Tarnus, Céline; Bertsch, Christophe; Chong, Julie

    2017-01-01

    Grapevine trunk diseases: Eutypa dieback, esca and Botryosphaeria dieback, which incidence has increased recently, are associated with several symptoms finally leading to the plant death. In the absence of efficient treatments, these diseases are a major problem for the viticulture; however, the factors involved in disease progression are not still fully identified. In order to get a better understanding of Botryosphaeria dieback development in grapevine, we have investigated different factors involved in Botryosphaeriaceae fungi aggressiveness. We first evaluated the activity of the wood-degrading enzymes of different isolates of Neofusicoccum parvum and Diplodia seriata, two major fungi associated with Botryosphaeria dieback. We further examinated the ability of these fungi to metabolize major grapevine phytoalexins: resveratrol and δ-viniferin. Our results demonstrate that Botryosphaeriaceae were characterized by differential wood decay enzymatic activities and have the capacity to rapidly degrade stilbenes. N. parvum is able to degrade parietal polysaccharides, whereas D. seriata has a better capacity to degrade lignin. Growth of both fungi exhibited a low sensitivity to resveratrol, whereas δ-viniferin has a fungistatic effect, especially on N. parvum Bourgogne S-116. We further show that Botryosphaeriaceae are able to metabolize rapidly resveratrol and δ-viniferin. The best stilbene metabolizing activity was measured for D. seriata. In conclusion, the different Botryosphaeriaceae isolates are characterized by a specific aggressiveness repertory. Wood and phenolic compound decay enzymatic activities could enable Botryosphaeriaceae to bypass chemical and physical barriers of the grapevine plant. The specific signature of Botryosphaeriaceae aggressiveness factors could explain the importance of fungi complexes in synergistic activity in order to fully colonize the host.

  7. First insights into macro- and meiofaunal colonisation patterns on paired wood/slate substrata at Atlantic deep-sea hydrothermal vents

    NASA Astrophysics Data System (ADS)

    Cuvelier, Daphne; Beesau, Julie; Ivanenko, Viatcheslav N.; Zeppilli, Daniela; Sarradin, Pierre-Marie; Sarrazin, Jozée

    2014-05-01

    In 2006, paired wood and slate panels, each equipped with a temperature probe, were deployed on three different localities on and around the Eiffel Tower edifice (Lucky Strike vent field, Mid-Atlantic Ridge) within close proximity of visible hydrothermal activity. Recovery of these panels took place in 2008. For this two-year deployment period, the composition of colonising organisms (both macro-and meiofauna) was assessed, along with image analyses of the deployment sites in 2006 and 2008. Very few significant differences in colonisation between organic (wood) and inorganic (slate) panels were revealed. Rather, the locality of deployment and the local environmental conditions and hydrothermal activity were found to influence taxonomic composition. Variability in microhabitat conditions and biological interactions were hypothesised to interact jointly in shaping new faunal communities on the colonisation substrata.

  8. [Medical certification in workers involved in logging and wood-processing].

    PubMed

    Romankow, Jacek

    2007-01-01

    Activities involved in forestry and woodworking industry are associated with workers being exposed to numerous environmental and technology-related factors that are detrimental to their health. Such hazards include working in changeable climatic conditions, in the vicinity of heavy equipment, exposure to noise, chainsaw vibrations, enforced body positioning, hard physical work, the effect of exhaust gases, potential effects of biological factors, including epizootic diseases. Wood processing involves performing mechanical activities employing tools and machines, as well as processes utilizing various chemical substances. Forestry and woodworking industry workers may deal both with timber and with wood products. In medical certification, the following issues are of significance: work in the vicinity of rotational elements, noise, effects of chemicals or biological factors, including carcinogenic substances. For this reason, the procedures involved in medical examinations of such workers are complex.

  9. Non-omnia moriantur-toxicity of mancozeb on dead wood microarthropod fauna.

    PubMed

    Adamski, Zbigniew; Bloszyk, Jerzy; Bruin, Jan; Ziemnicki, Kazimierz

    2007-01-01

    The effect of Dithane M-45 (dithiocarbamate fungicide; active substance: mancozeb) was studied on microarthropod fauna inhabiting dead wood. Although the exposure was almost never 100% lethal for the majority of observed taxa, almost all (Mesostigmata, Oribatida, some Uropodina, Actinedida, Collembola and Diplopoda) showed very high correlation between concentration of the fungicide and mortality (r > 0.86). Only Stigmaeidae showed low correlation (r = 0.293). For the majority of taxa LC(50 )values were close to the concentrations used during agrochemical activities in woods. Only Trachytes aegrota showed full susceptibility to the fungicide within the range of recommended field concentrations used in forestry (characterised by the low LC(95 )value). Tolerance of mesostigmatid and oribatid mites was found to differ between juveniles and adults, but not consistently. Related Uropodina species varied in susceptibility to the fungicide.

  10. Relations between water balance, wood traits and phenological behavior of tree species from a tropical dry forest in Costa Rica--a multifactorial study.

    PubMed

    Worbes, Martin; Blanchart, Sofie; Fichtler, Esther

    2013-05-01

    Drought tolerance is a key factor for the establishment and survival of tree species in tropical ecosystems. Specific mechanisms of drought resistance can be grouped into four functional ecotypes based on differences in leaf fall behavior: deciduous, brevi-deciduous, stem succulent and evergreen. To identify the key factors influencing phenology and cambial activity and thus drought tolerance, we tested the stomatal conductance, leaf water potential and stable carbon isotopes in the leaves and wood of 12 species from a tropical dry forest in Costa Rica. With wood anatomical techniques, we further studied seasonal cambial activity and a suite of wood traits related to water transport for each of the functional ecotypes. Using a principal component analysis, we identified two groups of variables that can be related to (i) hydraulic conductivity and (ii) control of transpiration and water loss. Hydraulic conductivity is controlled by vessel size as the limiting variable, water potential as the driving force and wood density as the stabilizing factor of the anatomical structure of an effective water transport system. Stomatal control plays a major role in terms of water loss or saving and is the dominant factor for differences in phenological behavior. Stem succulent species in particular developed a rarely identified but highly effective strategy against drought stress, which makes it a successful pioneer species in tropical dry forests.

  11. Functional Characterization of the Poplar R2R3-MYB Transcription Factor PtoMYB216 Involved in the Regulation of Lignin Biosynthesis during Wood Formation

    PubMed Central

    Lu, Wanxiang; Yang, Li; Jiang, Yuanzhong; Luo, Keming

    2013-01-01

    Because of the importance of wood in many industrial applications, tremendous studies have been performed on wood formation, especially in lignin biosynthesis. MYB transcription factors (TFs), which consist of a large family of plant TFs, have been reported to directly regulate lignin biosynthetic genes in a number of plants. In this study, we describe the cloning and functional characterization of PtoMYB216, a cDNA isolated from Chinese white poplar (Populus tomentosa Carr.). PtoMYB216 encodes a protein belonging to the R2R3-MYB family and displays significant similarity with other MYB factors shown to regulate lignin synthesis in Arabidopsis. Gene expression profiling studies showed that PtoMYB216 mRNA is specifically expressed during secondary wall formation in wood. The 1.8-kb promoter sequence of PtoMYB216 was fused to the GUS coding sequence and introduced into wild-type A. thaliana. GUS expression was shown to be restricted to tissues undergoing secondary cell wall formation. Overexpression of PtoMYB216 specifically activated the expression of the upstream genes in the lignin biosynthetic pathway and resulted in ectopic deposition of lignin in cells that are normally unligninified. These results suggest that PtoMYB216 is specific transcriptional activators of lignin biosynthesis and involved in the regulation of wood formation in poplar. PMID:24204619

  12. Lignocellulose-converting enzyme activity profiles correlate with molecular systematics and phylogeny grouping in the incoherent genus Phlebia (Polyporales, Basidiomycota).

    PubMed

    Kuuskeri, Jaana; Mäkelä, Miia R; Isotalo, Jarkko; Oksanen, Ilona; Lundell, Taina

    2015-10-19

    The fungal genus Phlebia consists of a number of species that are significant in wood decay. Biotechnological potential of a few species for enzyme production and degradation of lignin and pollutants has been previously studied, when most of the species of this genus are unknown. Therefore, we carried out a wider study on biochemistry and systematics of Phlebia species. Isolates belonging to the genus Phlebia were subjected to four-gene sequence analysis in order to clarify their phylogenetic placement at species level and evolutionary relationships of the genus among phlebioid Polyporales. rRNA-encoding (5.8S, partial LSU) and two protein-encoding gene (gapdh, rpb2) sequences were adopted for the evolutionary analysis, and ITS sequences (ITS1+5.8S+ITS2) were aligned for in-depth species-level phylogeny. The 49 fungal isolates were cultivated on semi-solid milled spruce wood medium for 21 days in order to follow their production of extracellular lignocellulose-converting oxidoreductases and carbohydrate active enzymes. Four-gene phylogenetic analysis confirmed the polyphyletic nature of the genus Phlebia. Ten species-level subgroups were formed, and their lignocellulose-converting enzyme activity profiles coincided with the phylogenetic grouping. The highest enzyme activities for lignin modification (manganese peroxidase activity) were obtained for Phlebia radiata group, which supports our previous studies on the enzymology and gene expression of this species on lignocellulosic substrates. Our study implies that there is a species-level connection of molecular systematics (genotype) to the efficiency in production of both lignocellulose-converting carbohydrate active enzymes and oxidoreductases (enzyme phenotype) on spruce wood. Thus, we may propose a similar phylogrouping approach for prediction of lignocellulose-converting enzyme phenotypes in new fungal species or genetically and biochemically less-studied isolates of the wood-decay Polyporales.

  13. Wood-Graphene Oxide Composite for Highly Efficient Solar Steam Generation and Desalination.

    PubMed

    Liu, Keng-Ku; Jiang, Qisheng; Tadepalli, Sirimuvva; Raliya, Ramesh; Biswas, Pratim; Naik, Rajesh R; Singamaneni, Srikanth

    2017-03-01

    Solar steam generation is a highly promising technology for harvesting solar energy, desalination and water purification. We introduce a novel bilayered structure composed of wood and graphene oxide (GO) for highly efficient solar steam generation. The GO layer deposited on the microporous wood provides broad optical absorption and high photothermal conversion resulting in rapid increase in the temperature at the liquid surface. On the other hand, wood serves as a thermal insulator to confine the photothermal heat to the evaporative surface and to facilitate the efficient transport of water from the bulk to the photothermally active space. Owing to the tailored bilayer structure and the optimal thermo-optical properties of the individual components, the wood-GO composite structure exhibited a solar thermal efficiency of ∼83% under simulated solar excitation at a power density of 12 kW/m 2 . The novel composite structure demonstrated here is highly scalable and cost-efficient, making it an attractive material for various applications involving large light absorption, photothermal conversion and heat localization.

  14. [The health impact of woodsmoke].

    PubMed

    Masse, Roland; Boudène, Claude

    2013-01-01

    Biomass is an important renewable resource of primary energy. Wood burning is expected to expand in future as a cheap means of residential heating, especially in wood-producing rural areas. The health consequences of wood smoke are taken in account far less actively in Europe than in North America, where stringent limitations have been introduced, such as those of the EPA Burnwise project. Recently, wildfires were estimated to release 2 petagrams of carbon into the atmosphere worldwide, contributing to more than 300 000 deaths annually. More effective measures are thus needed to prevent indoor and outdoor exposure to wood smoke. Ultrafine particles are the biggest concern, but the volatile phase also contains many toxicants. Ultrafine particles are among the most dangerous carbonaceous particles found in polluted atmospheres. They can be identified by using C14, levoglucosan, crystallography and spectrometry. Any expansion of the use of wood as a fuel source should take into account its health impact, which is similar to that of fossil fuels.

  15. Study of processes influencing bioavailability of pesticides in wood-soil systems: Effect of different factors.

    PubMed

    Marín-Benito, J M; Herrero-Hernández, E; Rodríguez-Cruz, M S; Arienzo, M; Sánchez-Martín, M J

    2017-05-01

    Lignocellulosic wastes and by-products containing lignin are now available in large amounts from forestry and industrial activities, and could be promising organic materials for the biosorption of pesticides by soils in order to reduce point-source pollution. Adding these materials to soil requires understanding the process of pesticide sorption-desorption by wood-soils, as sorption capacity could increase, with changes in pesticide bioavailability and final fate. The objective of this work was to study the effect that pine and oak wood added to soils had on the sorption/desorption of the pesticides linuron, alachlor, and metalaxyl. Experiments were conducted with two sandy loam and sandy clay soils each amended with two wood doses (5% and 50%) after different incubation times (0, 5 and 12 months). A low wood dose (5%) had no significant impact on the sorption (K f ) of alachlor, but K f increased for linuron (up to 5.4-1.7 times) and metalaxyl (up to 4.4 and 8.6 times) in all wood-soil systems. The results were not significantly different after different incubation times. The desorption results indicated that wood decreases the sorption irreversibility of alachlor, and increases that of linuron and metalaxyl, with a varying effect of the wood-soil incubation time. The addition of a high wood dose to soil (50%) was more significant for increasing the sorption of all the pesticides, and the sorbed amounts remaining after desorption (>49% for linuron, >33% for alachlor and >6% for metalaxyl), although there was no apparent discrimination between the two types of woods. The role of the nature of the organic carbón (K oc values) for sorption was evidenced for alachlor and metalaxyl, but not for linuron. These outcomes are of interest for extending wood application to soil as a barrier for avoiding environmental risk by point-source pollution due to the use and management of pesticides in farming systems. Copyright © 2017 Elsevier Inc. All rights reserved.

  16. Genomewide analysis of polysaccharides degrading enzymes in 11 white- and brown-rot Polyporales provides insight into mechanisms of wood decay

    Treesearch

    Chiaki Hori; Jill Gaskell; Kiyohiko Igarashi; Masahiro Samejima; David Hibbett; Bernard Henrissat; Dan Cullen

    2013-01-01

    To degrade the polysaccharides, wood-decay fungi secrete a variety of glycoside hydrolases (GHs) and carbohydrate esterases (CEs) classified into various sequence-based families of carbohydrate-active enzymes (CAZys) and their appended carbohydrate-binding modules (CBM). Oxidative enzymes, such as cellobiose dehydrogenase (CDH) and lytic polysaccharide monooxygenase (...

  17. Using rainfall simulators to test wood shreddings for erosion control

    Treesearch

    James Gronier; Randy Foltz; Charlie Showers

    2005-01-01

    The U.S. Department of Agriculture Forest Service is considering alternative methods of erosion control when constructing roads, decommissioning roads, protecting lands burned by wildland fires, and reclaiming lands disturbed by other activities. This article is the second in a series of tech tips that discuss the use of wood shreddings for erosion control. The first...

  18. Wood as inspiration for new stimuli-responsive structures and materials

    Treesearch

    Joseph E. Jakes; Nayomi Plaza-Rodriguez; Samuel L. Zelinka; Donald S. Stone; Sophie-Charlotte Gleber; Stefan Vogt

    2014-01-01

    Nature has often provided inspiration for new smart structures and materials. Recently, we showed a bundle of a few wood cells are moisture-activated torsional actuators that can reversibly twist multiple revolutions per centimeter of length. The bundles produce specific torque higher than that produced by electric motors and possess shape memory twist capabilities....

  19. Interacting effects of insects and flooding on wood decomposition.

    Treesearch

    Michael Ulyshen

    2014-01-01

    Saproxylic arthropods are thought to play an important role in wood decomposition but very few efforts have been made to quantify their contributions to the process and the factors controlling their activities are not well understood. In the current study, mesh exclusion bags were used to quantify how arthropods affect loblolly pine (Pinus taeda L.) decomposition rates...

  20. Management for esthetics and recreation, forage, water, and wildlife

    Treesearch

    Norbert V. DeByle

    1985-01-01

    In the West, aspen forests have not been actively managed for wood products largely because of the lack of markets for quaking aspen timber from the Rocky Mountains (see the WOOD UTILIZATION chapter). Despite this, the aspen ecosystem has been used to provide a variety of resources and opportunities (see PART 111. RESOURCES AND USES).

  1. Efficacy of copper borax preservative against wood decay

    Treesearch

    William Abbott; Bessie Woodward; Michael West

    2001-01-01

    In this study, a wood preservative containing active ingredients of 43.5% borax and 3.1% copper hydroxide was evaluated in soil-block tests in accordance with AWPA E10. Results suggested thatthe copper hydroxide was not contributing to fungal toxicity at preservative threshold levels. Thresholds determined for this preservative were very close to those previously...

  2. Comparative analysis of lignin peroxidase and manganese peroxidase activity on coniferous and deciduous wood using ToF-SIMS.

    PubMed

    MacDonald, Jacqueline; Goacher, Robyn E; Abou-Zaid, Mamdouh; Master, Emma R

    2016-09-01

    White-rot fungi are distinguished by their ability to efficiently degrade lignin via lignin-modifying type II peroxidases, including manganese peroxidase (MnP) and lignin peroxidase (LiP). In the present study, time-of flight secondary ion mass spectrometry (ToF-SIMS) was used to evaluate lignin modification in three coniferous and three deciduous wood preparations following treatment with commercial preparations of LiP and MnP from two different white-rot fungi. Percent modification of lignin was calculated as a loss of intact methoxylated lignin over nonfunctionalized aromatic rings, which is consistent with oxidative cleavage of methoxy moieties within the lignin structure. Exposure to MnP resulted in greater modification of lignin in coniferous compared to deciduous wood (28 vs. 18 % modification of lignin); and greater modification of G-lignin compared to S-lignin within the deciduous wood samples (21 vs. 12 %). In contrast, exposure to LiP resulted in similar percent modification of lignin in all wood samples (21 vs 22 %), and of G- and S-lignin within the deciduous wood (22 vs. 23 %). These findings suggest that the selected MnP and LiP may particularly benefit delignification of coniferous and deciduous wood, respectively. Moreover, the current analysis further demonstrates the utility of ToF-SIMS for characterizing enzymatic modification of lignin in wood fibre along with potential advantages over UV and HPCL-MS detection of solubilized delignification products.

  3. Fungal and bacterial community succession differs for three wood types during decay in a forest soil.

    PubMed

    Prewitt, Lynn; Kang, Youngmin; Kakumanu, Madhavi L; Williams, Mark

    2014-08-01

    Wood decomposition by soil microorganisms is vital to carbon and nutrient cycles of forested ecosystems. Different wood types decompose at different rates; however, it is not known if there are differences in microbial community succession associated with the decay of different wood types. In this study, the microbial community associated with the decay of pine (decay-susceptible wood), western red cedar (decay resistant) and ACQ-treated pine (Ammoniacal Copper Quaternary, preservative-treated pine for decay resistance) in forest soil was characterized using DNA sequencing, phospholipid fatty acid (PLFA) analysis, and microbial activity over a 26-month period. Bray-Curtis ordination using an internal transcribed spacer (ITS) sequence and PLFA data indicated that fungal communities changed during succession and that wood type altered the pattern of succession. Nondecay fungi decreased over the 26 months of succession; however, by 18 months of decay, there was a major shift in the fungal communities. By this time, Trametes elegans dominated cedar and Phlebia radiata dominated pine and ACQ-treated pine. The description of PLFA associated with ACQ-treated pine resembled cedar more than pine; however, both PLFA and ITS descriptions indicated that fungal communities associated with ACQ-treated pine were less dynamic, perhaps a result of the inhibition by the ACQ preservative, compared with pine and cedar. Overall, fungal community composition and succession were associated with wood type. Further research into the differences in community composition will help to discern their functional importance to wood decay.

  4. Porous texture of activated carbons prepared by phosphoric acid activation of woods

    NASA Astrophysics Data System (ADS)

    Díaz-Díez, M. A.; Gómez-Serrano, V.; Fernández González, C.; Cuerda-Correa, E. M.; Macías-García, A.

    2004-11-01

    Activated carbons (ACs) have been prepared using chestnut, cedar and walnut wood shavings from furniture industries located in the Comunidad Autónoma de Extremadura (SW Spain). Phosphoric acid (H3PO4) at different concentrations (i.e. 36 and 85 wt.%) has been used as activating agent. ACs have been characterized from the results obtained by N2 adsorption at 77 K. Moreover, the fractal dimension (D) has been calculated in order to determine the AC surface roughness degree. Optimal textural properties of ACs have been obtained by chemical activation with H3PO4 36 wt.%. This is corroborated by the slightly lower values of D for samples treated with H3PO4 85 wt.%.

  5. Evaluation of cellular effects of fine particulate matter from combustion of solid fuels used for indoor heating on the Navajo Nation using a stratified oxidative stress response model

    NASA Astrophysics Data System (ADS)

    Li, Ning; Champion, Wyatt M.; Imam, Jemal; Sidhu, Damansher; Salazar, Joseph R.; Majestic, Brian J.; Montoya, Lupita D.

    2018-06-01

    Communities in the Navajo Nation face public health burdens caused in part by the combustion of wood and coal for indoor heating using stoves that are old or in disrepair. Wood and coal combustion emits particulate matter (PM) with aerodynamic diameter < 2.5 μm (PM2.5), which can reach deep in the lung and cause injuries. Currently, there is little information about the health effects of wood and coal combustion-derived PM2.5 on Navajo Nation residents. This study tested the hypothesis that PM2.5 generated from solid fuel combustion in stoves commonly used by Navajo residents would induce stratified oxidative stress responses ranging from activation of antioxidant defense to inflammation and cell death in mouse macrophages (RAW 264.7). PM2.5 emitted from burning Ponderosa Pine (PP) and Utah Juniper (UJ) wood and Black Mesa (BM) and Fruitland (FR) coal in a stove representative of those widely used by Navajo residents were collected, and their aqueous suspensions used for cellular exposure. PM from combustion of wood had significantly more elemental carbon (EC) (15%) and soluble Ni (0.0029%) than the samples from coal combustion (EC: 3%; Ni: 0.0019%) and was also a stronger activator of antioxidant enzyme heme oxygenase-1 (11-fold increase vs. control) than that from coal (5-fold increase). Only PM from PP-wood (12-fold) and BM-coal (3-fold) increased the release of inflammatory cytokine tumor necrosis factor alpha. Among all samples, PP-wood consistently had the strongest oxidative stress and inflammatory effects. PM components, i.e. low-volatility organic carbon, EC, Cu, Ni and K were positively correlated with the cellular responses. Results showed that, at the concentrations tested, emissions from all fuels did not have significant cytotoxicity. These findings suggest that PM2.5 emitted from combustion of wood and coal commonly used by Navajo residents may negatively impact the health of this community.

  6. Comparison of NOx Removal Efficiencies in Compost Based Biofilters Using Four Different Compost Sources

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lacey, Jeffrey Alan; Lee, Brady Douglas; Apel, William Arnold

    2001-06-01

    In 1998, 3.6 trillion kilowatt-hours of electricity were generated in the United States. Over half of this was from coal-fired power plants, resulting in more than 8.3 million tons of nitrogen oxide (NOx) compounds being released into the environment. Over 95% of the NOx compounds produced during coal combustion are in the form of nitric oxide (NO). NOx emission regulations are becoming increasingly stringent, leading to the need for new, cost effective NOx treatment technologies. Biofiltration is such a technology. NO removal efficiencies were compared in compost based biofilters using four different composts. In previous experiments, removal efficiencies were typicallymore » highest at the beginning of the experiment, and decreased as the experiments proceeded. This work tested different types of compost in an effort to find a compost that could maintain NO removal efficiencies comparable to those seen early in the previous experiments. One of the composts was wood based with manure, two were wood based with high nitrogen content sludge, and one was dairy compost. The wood based with manure and one of the wood based with sludge composts were taken directly from an active compost pile while the other two composts were received in retail packaging which had been out of active piles for an indeterminate amount of time. A high temperature (55-60°C) off-gas stream was treated in biofilters operated under denitrifying conditions. Biofilters were operated at an empty bed residence time of 13 seconds with target inlet NO concentrations of 500 ppmv. Lactate was the carbon and energy source. Compost was sampled at 10-day intervals to determine aerobic and anaerobic microbial densities. Compost was mixed at a 1:1 ratio with lava rock and calcite was added at 100g/kg of compost. In each compost tested, the highest removal efficiencies occurred within the first 10 days of the experiment. The wood based with manure peaked at day 3 (77.14%), the dairy compost at day 1 (80.74%), the active wood based with sludge at day 5 (68.15%) and the inactive wood based with sludge at day 9 (63.64%, this compost was frozen when received). These levels gradually decreased throughout the remainder of the experiment until they fell between 40% and 60%. Decreasing removal efficiency was characteristic of all the composts tested, regardless of their makeup or activity state prior to testing. Although microbial densities and composition between composts may have differed, there was little change in densities within each experiment.« less

  7. Bioplastic production using wood mill effluents as feedstock.

    PubMed

    Ben, M; Mato, T; Lopez, A; Vila, M; Kennes, C; Veiga, M C

    2011-01-01

    Fibreboard production is one of the most important industrial activities in Galicia (Spain). Great amounts of wastewater are generated, with properties depending on the type of wood, treatment process, final product and water reusing, among others. These effluents are characterized by a high chemical oxygen demand, low pH and nutrients limitation. Although anaerobic digestion is one of the most suitable processes for the treatment, lately bioplastics production (mainly polyhydroxyalkanoates) from wastewaters with mixed cultures is being evaluated. Substrate requirements for these processes consist of high organic matter content and low nutrient concentration. Therefore, wood mill effluents could be a suitable feedstock. In this work, the possibility of producing bioplastics from to wood mill effluents is evaluated. First, wood mill effluent was converted to volatile fatty acids in an acidogenic reactor operated at two different hydraulic retention times of 1 and 1.5 d. The acidification percentage obtained was 37% and 42%, respectively. Then, aerobic batch assays were performed using fermented wood mill effluents obtained at different hydraulic retention times. Assays were developed using different cultures as inoculums. The maximum storage yield of 0.57 Cmmol/Cmmol was obtained when when the culture was enriched on a synthetic media.

  8. N-fertilization has different effects on the growth, carbon and nitrogen physiology, and wood properties of slow- and fast-growing Populus species

    PubMed Central

    Luo, Zhi-Bin

    2012-01-01

    To investigate how N-fertilization affects the growth, carbon and nitrogen (N) physiology, and wood properties of poplars with contrasting growth characteristics, slow-growing (Populus popularis, Pp) and fast-growing (P. alba×P. glandulosa, Pg) poplar saplings were exposed to different N levels. Above-ground biomass, leaf area, photosynthetic rates (A), instantaneous photosynthetic nitrogen use efficiency (PNUE i), chlorophyll and foliar sugar concentrations were higher in Pg than in Pp. Foliar nitrate reductase (NR) activities and root glutamate synthase (GOGAT) activities were higher in Pg than in Pp as were the N amount and NUE of new shoots. Lignin contents and calorific values of Pg wood were less than that of Pp wood. N-fertilization reduced root biomass of Pg more than of Pp, but increased leaf biomass, leaf area, A, and PNUEi of Pg more than of Pp. Among 13 genes involved in the transport of ammonium or nitrate or in N assimilation, transcripts showed more pronounced changes to N-fertilization in Pg than in Pp. Increases in NR activities and N contents due to N-fertilization were larger in Pg than in Pp. In both species, N-fertilization resulted in lower calorific values as well as shorter and wider vessel elements/fibres. These results suggest that growth, carbon and N physiology, and wood properties are more sensitive to increasing N availability in fast-growing poplars than in slow-growing ones, which is probably due to prioritized resource allocation to the leaves and accelerated N physiological processes in fast-growing poplars under higher N levels. PMID:23028021

  9. N-fertilization has different effects on the growth, carbon and nitrogen physiology, and wood properties of slow- and fast-growing Populus species.

    PubMed

    Li, Hong; Li, Mengchun; Luo, Jie; Cao, Xu; Qu, Long; Gai, Ying; Jiang, Xiangning; Liu, Tongxian; Bai, Hua; Janz, Dennis; Polle, Andrea; Peng, Changhui; Luo, Zhi-Bin

    2012-10-01

    To investigate how N-fertilization affects the growth, carbon and nitrogen (N) physiology, and wood properties of poplars with contrasting growth characteristics, slow-growing (Populus popularis, Pp) and fast-growing (P. alba×P. glandulosa, Pg) poplar saplings were exposed to different N levels. Above-ground biomass, leaf area, photosynthetic rates (A), instantaneous photosynthetic nitrogen use efficiency (PNUE (i)), chlorophyll and foliar sugar concentrations were higher in Pg than in Pp. Foliar nitrate reductase (NR) activities and root glutamate synthase (GOGAT) activities were higher in Pg than in Pp as were the N amount and NUE of new shoots. Lignin contents and calorific values of Pg wood were less than that of Pp wood. N-fertilization reduced root biomass of Pg more than of Pp, but increased leaf biomass, leaf area, A, and PNUE(i) of Pg more than of Pp. Among 13 genes involved in the transport of ammonium or nitrate or in N assimilation, transcripts showed more pronounced changes to N-fertilization in Pg than in Pp. Increases in NR activities and N contents due to N-fertilization were larger in Pg than in Pp. In both species, N-fertilization resulted in lower calorific values as well as shorter and wider vessel elements/fibres. These results suggest that growth, carbon and N physiology, and wood properties are more sensitive to increasing N availability in fast-growing poplars than in slow-growing ones, which is probably due to prioritized resource allocation to the leaves and accelerated N physiological processes in fast-growing poplars under higher N levels.

  10. Synthesis of D-glucosamine quaternary ammonium derivatives and evaluation of their antifungal activity together with aminodeoxyglucose derivatives against two wood fungi Coriolus versicolor and Poria placenta: structure-activity relationships.

    PubMed

    Muhizi, Théoneste; Coma, Véronique; Grelier, Stéphane

    2011-03-01

    Structure-activity relationships are often reported in scientific studies. These may be employed in searching for new acceptable biocides to use against harmful microorganisms, because the biocides used hitherto encounter various problems, including lack of efficiency, high toxicity and persistence. Nowadays, scientists are trying to find new, environmentally acceptable biocides to replace these earlier biocides. Different compounds from renewable materials have been studied and have shown pronounced antifungal activity against wood fungi. These include aminopolysaccharide derivatives and different quaternary ammonium polymers. A biological study carried out with these products indicated a possible relationship between amino groups and differences in biological activity observed. In this study, an amino group was successively fixed to different carbon atoms of glucose, and glucosamine was also modified by both N-alkylation and quaternisation. The impact of the amino group position on antifungal activity against two wood decay fungi was investigated. The amino group at the anomeric position showed the highest antifungal activity against both Coriolus versicolor Quel. and Poria placenta (Fr.) Cooke. Furthermore, the positive impact of both N-alkylation and quaternisation on the growth of both strains was demonstrated. The anomeric position of the amino group and the N-alkylation and quaternisation of amino sugars considerably increase the antifungal activity of these compounds. Copyright © 2010 Society of Chemical Industry.

  11. Identification of endangered or threatened Costa Rican tree species by wood anatomy and fluorescence activity.

    PubMed

    Moya, Róger; Wiemann, Michael C; Olivares, Carlos

    2013-09-01

    A total of 45 native Costa Rican tree species are threatened or in danger of extinction, but the Convention on International Trade Endangered Species (CITES) includes only eight of these in its Appendices. However, the identification of other species based on their wood anatomy is limited. The present study objective was to describe and to compare wood anatomy and fluorescence activity in some endangered or threatened species of Costa Rica. A total of 45 (22 endangered and 23 threatened with extinction) wood samples of these species, from the xylaria of the Instituto Tecnológico de Costa Rica and the Forest Products Laboratory in Madison, Wisconsin, were examined. Surface fluorescence was positive in eight species, water extract fluorescence was positive in six species and ethanol extract fluorescence was positive in 24 species. Almost all species were diffuse porous except for occasional (Cedrela odorata, C. fissilis, Cordia gerascanthus) or regular (C. salvadorensis and C. tonduzii) semi-ring porosity. A dendritic vessel arrangement was found in Sideroxylon capari, and pores were solitary in Guaiacum sanctum and Vantanea barbourii. Vessel element length was shortest in Guaiacum sanctum and longest in Humiriastrum guianensis, Minquartia guianensis and Vantanea barbourii. Finally, anatomical information and fluorescence activity were utilized to construct an identification key of species, in which fluorescence is a feature used in identification.

  12. RP-HPLC-DAD-ESI-QTOF-MS based metabolic profiling of the potential Olea europaea by-product "wood" and its comparison with leaf counterpart.

    PubMed

    Ammar, Sonda; Contreras, Maria Del Mar; Gargouri, Boutheina; Segura-Carretero, Antonio; Bouaziz, Mohamed

    2017-05-01

    Olea europaea L. organs such as leaves, stems and roots have been associated with numerous in vivo and in vitro biological activities and used for traditional medicinal purposes. However, tree wood is an untapped resource with little information about their chemical composition. That is why, the objective of this study is to increase the knowledge about phytochemicals from 'Chemlali' olive wood by means of mass spectrometry-based analyses. Its comparison with by-products derived from leaves was also studied. Hydromethanol extracts from wood and leaves with stems of 'Chemlali' olive cultivar were analysed using reversed-phase (RP) high-performance liquid chromatography (HPLC) coupled to two detection systems: diode-array detection (DAD) and quadrupole time-of-flight (QTOF) mass spectrometry (MS) in negative ion mode. Tandem MS experiments were performed to establish the chemical structure of olive phytochemicals. A total of 85 compounds were characterised in the studied olive parts and classified as: sugars (3), organic acids (5), one phenolic aldehyde, simple phenolic acids (6), simple phenylethanoids (5), flavonoids (14), coumarins (3), caffeoyl phenylethanoid derivatives (6), iridoids (5), secoiridoids (32), and lignans (5). To our knowledge, the major part of these metabolites was not previously reported in olive tree wood, and 10 olive chemical constituents were identified for the first time in the Oleaceae family. The results presented here demonstrated the usefulness of the methodology proposed, based on RP-HPLC-DAD-ESI-QTOF-MS and MS/MS, to develop an exhaustive metabolic profiling and to recover new biologically active compounds in olive wood with pharmacologic and cosmetic potential. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

  13. The Economics of Forest Carbon Sequestration: The Challenge for Emissions Offset Trading

    NASA Astrophysics Data System (ADS)

    van Kooten, G. C.

    2016-12-01

    This paper provides an overview of the role that forestry activities can play in mitigating climate change. The price of carbon offset credits is used for incentivizing a reduction in the release of CO2 emissions and an increase in sequestration of atmospheric CO2 through forestry activities. Forestland owners essentially have two options for creating carbon offset credits: (1) avoid or delay harvest of mature timber; or (2) harvest timber and allow natural regeneration or regeneration with `regular' or genetically-enhanced growing stock, storing carbon in post-harvest products, using sawmill and potentially logging residues to generate electricity. In this study, a model representative of the Quesnel Timber Supply Area (TSA) in the BC interior is developed. The objective is to maximize net discounted returns to commercial timber operations (and sale of downstream products) plus the benefits of managing carbon fluxes. The model tracks carbon in living trees, organic matter, and, importantly, post-harvest carbon pools and avoided emissions from substituting wood for non-wood in construction or wood bioenergy for fossil fuels. Model constraints ensure that commercial forest management is sustainable, while carbon prices incentivize sequestration to ensure efficient mitigation of climate change. The results are confirmed more generally by comparing the carbon fluxes derived from the integrated forest management model with those from a Faustmann-Hartman rotation age model that explicitly includes benefits of storing carbon. One other question is addressed: If carbon offsets are created when wood biomass substitutes for fossil fuels in power generation, can one count the saved emissions from steel/cement production when wood substitutes for non-wood materials in construction?

  14. Synchrotron-based X-ray fluorescence microscopy enables multiscale spatial visualization of ions involved in fungal lignocellulose deconstruction

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kirker, Grant; Zelinka, Sam; Gleber, Sophie -Charlotte

    Ions play an important role in the growth and development of filamentous fungi, particularly in the fungal decay process of lignocellulose materials. The role of ions in wood degradation, and more broadly fungal metabolism, have implications for diverse research disciplines ranging from plant pathology and forest ecology, to wood protection. Despite the importance of ions in both enzymatic and non-enzymatic fungal decay mechanisms, the spatial distribution of ions in wood and fungal hyphae during decay is not known. Here we employ synchrotron based X-ray fluorescence microscopy (XFM) to map physiologically relevant ions, such as K, Ca, Mn, Fe, and Zn,more » in wood being decayed by the model brown rot fungus Serpula lacrymans. Two-dimensional XFM maps were obtained to study the ion spatial distributions from mm to submicron length scales in wood and hyphae. Three-dimensional ion volume reconstructions with submicron spatial resolution were also acquired of wood cell walls and fungal hyphae, and an estimation of oxalate concentration at the microscale was made. Results show that the fungus actively transports some ions, such as Fe, into the wood and controls the distribution of ions at both the bulk wood and cellular length scales. Within the fungal hyphae, ion volume reconstructions show inhomogeneous ion distributions at the micron length scale and this localization may be indicative of both physiological status and requirements or in some cases, potentially sites associated with the initiation of metal-catalyzed wood degradation. Finally, these measurements illustrate how synchrotron based XFM is uniquely qualified for probing the role of ions in the growth and metabolic processes of filamentous fungi.« less

  15. Synchrotron-based X-ray fluorescence microscopy enables multiscale spatial visualization of ions involved in fungal lignocellulose deconstruction

    DOE PAGES

    Kirker, Grant; Zelinka, Sam; Gleber, Sophie -Charlotte; ...

    2017-01-31

    Ions play an important role in the growth and development of filamentous fungi, particularly in the fungal decay process of lignocellulose materials. The role of ions in wood degradation, and more broadly fungal metabolism, have implications for diverse research disciplines ranging from plant pathology and forest ecology, to wood protection. Despite the importance of ions in both enzymatic and non-enzymatic fungal decay mechanisms, the spatial distribution of ions in wood and fungal hyphae during decay is not known. Here we employ synchrotron based X-ray fluorescence microscopy (XFM) to map physiologically relevant ions, such as K, Ca, Mn, Fe, and Zn,more » in wood being decayed by the model brown rot fungus Serpula lacrymans. Two-dimensional XFM maps were obtained to study the ion spatial distributions from mm to submicron length scales in wood and hyphae. Three-dimensional ion volume reconstructions with submicron spatial resolution were also acquired of wood cell walls and fungal hyphae, and an estimation of oxalate concentration at the microscale was made. Results show that the fungus actively transports some ions, such as Fe, into the wood and controls the distribution of ions at both the bulk wood and cellular length scales. Within the fungal hyphae, ion volume reconstructions show inhomogeneous ion distributions at the micron length scale and this localization may be indicative of both physiological status and requirements or in some cases, potentially sites associated with the initiation of metal-catalyzed wood degradation. Finally, these measurements illustrate how synchrotron based XFM is uniquely qualified for probing the role of ions in the growth and metabolic processes of filamentous fungi.« less

  16. Wood decomposition as influenced by invertebrates.

    PubMed

    Ulyshen, Michael D

    2016-02-01

    The diversity and habitat requirements of invertebrates associated with dead wood have been the subjects of hundreds of studies in recent years but we still know very little about the ecological or economic importance of these organisms. The purpose of this review is to examine whether, how and to what extent invertebrates affect wood decomposition in terrestrial ecosystems. Three broad conclusions can be reached from the available literature. First, wood decomposition is largely driven by microbial activity but invertebrates also play a significant role in both temperate and tropical environments. Primary mechanisms include enzymatic digestion (involving both endogenous enzymes and those produced by endo- and ectosymbionts), substrate alteration (tunnelling and fragmentation), biotic interactions and nitrogen fertilization (i.e. promoting nitrogen fixation by endosymbiotic and free-living bacteria). Second, the effects of individual invertebrate taxa or functional groups can be accelerative or inhibitory but the cumulative effect of the entire community is generally to accelerate wood decomposition, at least during the early stages of the process (most studies are limited to the first 2-3 years). Although methodological differences and design limitations preclude meta-analysis, studies aimed at quantifying the contributions of invertebrates to wood decomposition commonly attribute 10-20% of wood loss to these organisms. Finally, some taxa appear to be particularly influential with respect to promoting wood decomposition. These include large wood-boring beetles (Coleoptera) and termites (Termitoidae), especially fungus-farming macrotermitines. The presence or absence of these species may be more consequential than species richness and the influence of invertebrates is likely to vary biogeographically. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.

  17. Longevity of Wood-Forced Pools in an Old-Growth Forest

    NASA Astrophysics Data System (ADS)

    Buffington, J. M.; Woodsmith, R. D.; Johnson, A. C.

    2009-12-01

    Wood debris plays an important role in scouring pools in forest channels and providing resultant habitat for aquatic organisms. We investigated the longevity of such pools in a gravel-bed river flowing through old-growth forest in southeastern Alaska by aging trees and “bear’s bread” fungi (Ganoderma applanatum, Fomitopsis pinicola) growing on pool-forming wood debris. Ages were determined by counting annual growth rings from cores and cross sections of trees and fungi growing on the wood debris. These ages are minimum values because they do not account for lag time between debris recruitment and seedling/spore establishment on the debris, nor do they account for flood scour that may periodically reset tree and fungi growth on the debris. The study stream has a gradient of about 1%, bankfull width and depth of 13.3 and 0.78 m, respectively, median grain size of 18 mm, a high wood loading (0.8 pieces/m), and a correspondingly low pool spacing (0.3 bankfull widths/pool), with 81% of the pools forced by wood debris. The size of wood debris in the study stream is large relative to the channel (average log length of 7.6 m and diameter of 0.35 m), rendering most debris immobile. Eighty-one pool-forming pieces of wood were dated over 1.2 km of stream length, with 28% of these pieces causing scour of more than one pool. In all, 122 wood-forced pools were dated, accounting for 38% of all pools at the site and 47% of the wood-forced pools. Fifty-three percent of the wood-forced pools lacked datable wood because these pieces either: were newly recruited; had been scoured by floods; or were contained below the active channel elevation, prohibiting vegetation establishment on the wood debris (the most common cause). The debris age distribution declined exponentially from 2 to 113 yrs., with a median value of 18 yrs. Similar exponential residence time distributions have been reported in other studies, but our analysis focused specifically on the ages of pool-forming wood as opposed to all in-channel wood. Most pool scour was relatively recent (60% ≤ 25 yrs. old), but 16% of the pools were old features (50-100+ yrs.), indicating long-term availability of pool habitats at the study site. Future studies will use these results to develop a wood budget model that accounts for pool scour and availability of pool habitats. For such modeling, our data suggest that stand-replacing disturbances (e.g. wildfire, riparian clear cutting) will cause a sharp drop in the numbers of wood-forced pools, as most of those are ≤ 25 yrs. old.

  18. Horizontal gene transfer and gene dosage drives adaptation to wood colonization in a tree pathogen

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Dhillon, Braham; Feau, Nicolas; Aerts, Andrea L.

    Some of the most damaging tree diseases are caused by pathogens that induce cankers, a stem deformation often lethal. To investigate the cause of this adaptation, we sequenced the genomes of poplar pathogens that do and do not cause cankers. We found a unique cluster of genes that produce secondary metabolites and are co-activated when the canker pathogen is grown on poplar wood and leaves. The gene genealogy is discordant with the species phylogeny, showing a signature of horizontal transfer from fungi associated with wood decay. Furthermore, genes encoding hemicellulose-degrading enzymes are up-regulated on poplar wood chips, with some havingmore » been acquired horizontally. In conclusion, we propose that adaptation to colonize poplar woody stems is the result of acquisition of these genes.« less

  19. Horizontal gene transfer and gene dosage drives adaptation to wood colonization in a tree pathogen

    DOE PAGES

    Dhillon, Braham; Feau, Nicolas; Aerts, Andrea L.; ...

    2015-03-02

    Some of the most damaging tree diseases are caused by pathogens that induce cankers, a stem deformation often lethal. To investigate the cause of this adaptation, we sequenced the genomes of poplar pathogens that do and do not cause cankers. We found a unique cluster of genes that produce secondary metabolites and are co-activated when the canker pathogen is grown on poplar wood and leaves. The gene genealogy is discordant with the species phylogeny, showing a signature of horizontal transfer from fungi associated with wood decay. Furthermore, genes encoding hemicellulose-degrading enzymes are up-regulated on poplar wood chips, with some havingmore » been acquired horizontally. In conclusion, we propose that adaptation to colonize poplar woody stems is the result of acquisition of these genes.« less

  20. Pectin methyl esterase inhibits intrusive and symplastic cell growth in developing wood cells of Populus.

    PubMed

    Siedlecka, Anna; Wiklund, Susanne; Péronne, Marie-Amélie; Micheli, Fabienne; Lesniewska, Joanna; Sethson, Ingmar; Edlund, Ulf; Richard, Luc; Sundberg, Björn; Mellerowicz, Ewa J

    2008-02-01

    Wood cells, unlike most other cells in plants, grow by a unique combination of intrusive and symplastic growth. Fibers grow in diameter by diffuse symplastic growth, but they elongate solely by intrusive apical growth penetrating the pectin-rich middle lamella that cements neighboring cells together. In contrast, vessel elements grow in diameter by a combination of intrusive and symplastic growth. We demonstrate that an abundant pectin methyl esterase (PME; EC 3.1.1.11) from wood-forming tissues of hybrid aspen (Populus tremula x tremuloides) acts as a negative regulator of both symplastic and intrusive growth of developing wood cells. When PttPME1 expression was up- and down-regulated in transgenic aspen trees, the PME activity in wood-forming tissues was correspondingly altered. PME removes methyl ester groups from homogalacturonan (HG) and transgenic trees had modified HG methylesterification patterns, as demonstrated by two-dimensional nuclear magnetic resonance and immunostaining using PAM1 and LM7 antibodies. In situ distributions of PAM1 and LM7 epitopes revealed changes in pectin methylesterification in transgenic trees that were specifically localized in expanding wood cells. The results show that en block deesterification of HG by PttPME1 inhibits both symplastic growth and intrusive growth. PttPME1 is therefore involved in mechanisms determining fiber width and length in the wood of aspen trees.

  1. Whole genome and global gene expression analyses of the model mushroom Flammulina velutipes reveal a high capacity for lignocellulose degradation.

    PubMed

    Park, Young-Jin; Baek, Jeong Hun; Lee, Seonwook; Kim, Changhoon; Rhee, Hwanseok; Kim, Hyungtae; Seo, Jeong-Sun; Park, Hae-Ran; Yoon, Dae-Eun; Nam, Jae-Young; Kim, Hong-Il; Kim, Jong-Guk; Yoon, Hyeokjun; Kang, Hee-Wan; Cho, Jae-Yong; Song, Eun-Sung; Sung, Gi-Ho; Yoo, Young-Bok; Lee, Chang-Soo; Lee, Byoung-Moo; Kong, Won-Sik

    2014-01-01

    Flammulina velutipes is a fungus with health and medicinal benefits that has been used for consumption and cultivation in East Asia. F. velutipes is also known to degrade lignocellulose and produce ethanol. The overlapping interests of mushroom production and wood bioconversion make F. velutipes an attractive new model for fungal wood related studies. Here, we present the complete sequence of the F. velutipes genome. This is the first sequenced genome for a commercially produced edible mushroom that also degrades wood. The 35.6-Mb genome contained 12,218 predicted protein-encoding genes and 287 tRNA genes assembled into 11 scaffolds corresponding with the 11 chromosomes of strain KACC42780. The 88.4-kb mitochondrial genome contained 35 genes. Well-developed wood degrading machinery with strong potential for lignin degradation (69 auxiliary activities, formerly FOLymes) and carbohydrate degradation (392 CAZymes), along with 58 alcohol dehydrogenase genes were highly expressed in the mycelium, demonstrating the potential application of this organism to bioethanol production. Thus, the newly uncovered wood degrading capacity and sequential nature of this process in F. velutipes, offer interesting possibilities for more detailed studies on either lignin or (hemi-) cellulose degradation in complex wood substrates. The mutual interest in wood degradation by the mushroom industry and (ligno-)cellulose biomass related industries further increase the significance of F. velutipes as a new model.

  2. Whole Genome and Global Gene Expression Analyses of the Model Mushroom Flammulina velutipes Reveal a High Capacity for Lignocellulose Degradation

    PubMed Central

    Park, Young-Jin; Baek, Jeong Hun; Lee, Seonwook; Kim, Changhoon; Rhee, Hwanseok; Kim, Hyungtae; Seo, Jeong-Sun; Park, Hae-Ran; Yoon, Dae-Eun; Nam, Jae-Young; Kim, Hong-Il; Kim, Jong-Guk; Yoon, Hyeokjun; Kang, Hee-Wan; Cho, Jae-Yong; Song, Eun-Sung; Sung, Gi-Ho; Yoo, Young-Bok; Lee, Chang-Soo; Lee, Byoung-Moo; Kong, Won-Sik

    2014-01-01

    Flammulina velutipes is a fungus with health and medicinal benefits that has been used for consumption and cultivation in East Asia. F. velutipes is also known to degrade lignocellulose and produce ethanol. The overlapping interests of mushroom production and wood bioconversion make F. velutipes an attractive new model for fungal wood related studies. Here, we present the complete sequence of the F. velutipes genome. This is the first sequenced genome for a commercially produced edible mushroom that also degrades wood. The 35.6-Mb genome contained 12,218 predicted protein-encoding genes and 287 tRNA genes assembled into 11 scaffolds corresponding with the 11 chromosomes of strain KACC42780. The 88.4-kb mitochondrial genome contained 35 genes. Well-developed wood degrading machinery with strong potential for lignin degradation (69 auxiliary activities, formerly FOLymes) and carbohydrate degradation (392 CAZymes), along with 58 alcohol dehydrogenase genes were highly expressed in the mycelium, demonstrating the potential application of this organism to bioethanol production. Thus, the newly uncovered wood degrading capacity and sequential nature of this process in F. velutipes, offer interesting possibilities for more detailed studies on either lignin or (hemi-) cellulose degradation in complex wood substrates. The mutual interest in wood degradation by the mushroom industry and (ligno-)cellulose biomass related industries further increase the significance of F. velutipes as a new model. PMID:24714189

  3. Applying management modeling to assess the feasibility of accelerating landscape restoration on federal forests in Eastern Oregon

    Treesearch

    Sara Loreno; Jeremy S. Fried; Andrew Yost

    2015-01-01

    The state of Oregon recently invested in exploring options for increasing the extent of forest restoration activity. This initiative aims to reduce the incidence, effects, and expense of catastrophic fire events and restore economic stability to rural communities by enhancing the supply of raw materials for wood processing facilities and wood-based, renewable energy...

  4. Proposed Expansion of Acme Landfill Operations.

    DTIC Science & Technology

    1982-08-01

    plastic beverage bottles, cardboard, wood, yardwastes, textiles , rubber, and leather. In addition to traditional activities, a central processing center...from food and garden wastes), a considerable portion of the leachate strength may be attributable to the textiles , rubber, leather, wood, paper, and...poisoning); mosquitos which may carry viral diseases such as encephalitis, malaria, and yellow fever; rodents which are carriers of enteric and other

  5. Plasma enhanced modification of TMP fiber and its effect on tensile strength of wood fiber/PP composite

    Treesearch

    Sangyeob Lee; Todd F. Shupe; Chung Y. Hse

    2009-01-01

    Plasma-assisted surface treatment on thermomechanical pulp (TMP) fiber and polypropylene (PP) film was investigated to obtain interfacial adhesion at the wood fiber and PP interface. A metal plate between electrodes prevented thermal damage to the TMP fiber handsheets and PP film. Oxygen-plasma treatment provided better surface activation on the TMP fiber and...

  6. 2009 Wood and Fiber Product Seminar : VTT and USDA joint activity

    Treesearch

    Ali Harlin; Minna Vikman

    2010-01-01

    Foward -- The development of high-value wood and fiber products is one of the most important challenges currently facing the forest industry. Traditional pulp and paper products are on a critical path in developed countries with prices and markets decreasing. Finland and the USA have faced the same problem, which is a fundamental reason for Industrial Biomaterials...

  7. West Virginia timber product output, 2000

    Treesearch

    Bruce Hansen; Ed Murriner; Iris Baker; Melody Akers; Melody Akers

    2006-01-01

    Assesses primary wood-processing activities in West Virginia for 2000. West Virginia?s total wood harvest for industrial uses was 202 million cubic feet, up nearly 22 percent from 1994. Sawlog production totaled 803.5 million board feet, a decrease of 8.1 million board feet from 1994. There were 172 sawmills operating in the State in 2000, with only 10 percent...

  8. Grapevine Botryosphaeria dieback fungi have specific aggressiveness factor repertory involved in wood decay and stilbene metabolization

    PubMed Central

    Wilhelm, Kim; Tarnus, Céline; Bertsch, Christophe

    2017-01-01

    Grapevine trunk diseases: Eutypa dieback, esca and Botryosphaeria dieback, which incidence has increased recently, are associated with several symptoms finally leading to the plant death. In the absence of efficient treatments, these diseases are a major problem for the viticulture; however, the factors involved in disease progression are not still fully identified. In order to get a better understanding of Botryosphaeria dieback development in grapevine, we have investigated different factors involved in Botryosphaeriaceae fungi aggressiveness. We first evaluated the activity of the wood-degrading enzymes of different isolates of Neofusicoccum parvum and Diplodia seriata, two major fungi associated with Botryosphaeria dieback. We further examinated the ability of these fungi to metabolize major grapevine phytoalexins: resveratrol and δ-viniferin. Our results demonstrate that Botryosphaeriaceae were characterized by differential wood decay enzymatic activities and have the capacity to rapidly degrade stilbenes. N. parvum is able to degrade parietal polysaccharides, whereas D. seriata has a better capacity to degrade lignin. Growth of both fungi exhibited a low sensitivity to resveratrol, whereas δ-viniferin has a fungistatic effect, especially on N. parvum Bourgogne S-116. We further show that Botryosphaeriaceae are able to metabolize rapidly resveratrol and δ-viniferin. The best stilbene metabolizing activity was measured for D. seriata. In conclusion, the different Botryosphaeriaceae isolates are characterized by a specific aggressiveness repertory. Wood and phenolic compound decay enzymatic activities could enable Botryosphaeriaceae to bypass chemical and physical barriers of the grapevine plant. The specific signature of Botryosphaeriaceae aggressiveness factors could explain the importance of fungi complexes in synergistic activity in order to fully colonize the host. PMID:29261692

  9. The decay of wood in landfills in contrasting climates in Australia

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ximenes, Fabiano, E-mail: fabiano.ximenes@dpi.nsw.gov.au; Björdal, Charlotte; Cowie, Annette

    Highlights: • We examine decay in wood from landfills in contrasting environments in Australia. • Analysis is based on changes in chemical composition and microscopy. • Climate did not influence levels of decay observed. • Microscopy of retrieved samples revealed most of the decay was aerobic in nature. • Current default factors for wood decay in landfills overestimate methane emissions. - Abstract: Wood products in landfill are commonly assumed to decay within several decades, returning the carbon contained therein to the atmosphere, with about half the carbon released as methane. However, the rate and extent of decay is not wellmore » known, as very few studies have examined the decay of wood products in landfills. This study reports on the findings from landfill excavations conducted in the Australian cities of Sydney and Cairns located in temperate and tropical environments, respectively. The objective of this study was to determine whether burial of the wood in warmer, more tropical conditions in Cairns would result in greater levels of decay than occurs in the temperate environment of Sydney. Wood samples recovered after 16–44 years in landfill were examined through physical, chemical and microscopic analyses, and compared with control samples to determine the carbon loss. There was typically little or no decay in the wood samples analysed from the landfill in Sydney. Although there was significant decay in rainforest wood species excavated from Cairns, decay levels for wood types that were common to both Cairns and Sydney landfills were similar. The current Intergovernmental Panel on Climate Change (IPCC, 2006) default decay factor for organic materials in landfills is 50%. In contrast, the carbon loss determined for Pinus radiata recovered from Sydney and Cairns landfills was 7.9% and 4.4%, respectively, and 0% for Agathis sp. This suggests that climate did not influence decay, and that the more extensive levels of decay observed for some wood samples from Cairns indicates that those wood types were more susceptible to biodegradation. Microscopic analyses revealed that most decay patterns observed in samples analysed from Sydney were consistent with aerobic fungal decay. Only a minor portion of the microbial decay was due to erosion bacteria active in anaerobic/near anaerobic environments. The findings of this study strongly suggest that models that adopt current accepted default factors for the decay of wood in landfills greatly overestimate methane emissions.« less

  10. Public participation GIS for improving wood burning emissions from residential heating and urban environmental management.

    PubMed

    López-Aparicio, Susana; Vogt, Matthias; Schneider, Philipp; Kahila-Tani, Maarit; Broberg, Anna

    2017-04-15

    A crowdsourcing study supported by a public participation GIS tool was designed and carried out in two Norwegian regions. The aim was to improve the knowledge about emissions from wood burning for residential heating in urban areas based on the collection of citizens' localized insights. We focus on three main issues: 1) type of dwelling and residential heating source; 2) wood consumption and type of wood appliances; and 3) citizens' perception of the urban environment. Our study shows the importance of wood burning for residential heating, and of the resulted particle emissions, in Norwegian urban areas. Citizens' localized insights on environmental perception highlight the areas in the city that require particular attention as part of clean air strategies. Information about environmental perception is combined with existing environmental data showing certain correlation. The results support the urban environmental management based on co-benefit approaches, achieving several outcomes from a single policy measure. Measures to reduce urban air pollution will have a positive impact on the citizens' environmental perception, and therefore on their quality of life, in addition to reducing the negative consequences of air pollution on human health. The characterization of residential heating by fuelwood is still a challenging activity. Our study shows the potential of a crowdsourcing method as means for bottom-up approaches designed to increase our knowledge on human activities at urban scale that result on emissions. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  11. The functional properties of a xyloglucanase (GH12) of Aspergillus terreus expressed in Aspergillus nidulans may increase performance of biomass degradation.

    PubMed

    Vitcosque, Gabriela Leal; Ribeiro, Liliane Fraga Costa; de Lucas, Rosymar Coutinho; da Silva, Tony Marcio; Ribeiro, Lucas Ferreira; de Lima Damasio, André Ricardo; Farinas, Cristiane Sanchez; Gonçalves, Aline Zorzetto Lopes; Segato, Fernando; Buckeridge, Marcos Silveira; Jorge, João Atilio; Polizeli, Maria de Lourdes T M

    2016-11-01

    Filamentous fungi are attractive hosts for heterologous protein expression due to their capacity to secrete large amounts of enzymes into the extracellular medium. Xyloglucanases, which specifically hydrolyze xyloglucan, have been recently applied in lignocellulosic biomass degradation and conversion in many other industrial processes. In this context, this work aimed to clone, express, and determine the functional properties of a recombinant xyloglucanase (AtXEG12) from Aspergillus terreus, and also its solid-state (SSF) and submerged (SmF) fermentation in bioreactors. The purified AtXEG12 showed optimum pH and temperature of 5.5 and 65 °C, respectively, demonstrating to be 90 % stable after 24 h of incubation at 50 °C. AtXEG12 activity increased in the presence of 2-mercaptoethanol (65 %) and Zn +2 (45 %), while Cu +2 and Ag + ions drastically decreased its activity. A substrate assay showed, for the first time for this enzyme's family, xylanase activity. The enzyme exhibited high specificity for tamarind xyloglucan (K M 1.2 mg mL -1 ) and V max of 17.4 μmol min -1  mg -1 of protein. The capillary zone electrophoresis analysis revealed that AtXEG12 is an endo-xyloglucanase. The heterologous xyloglucanase secretion was greater than the production by wild-type A. terreus cultivated in SmF. On the other hand, AtXEG12 activity reached by SSF was sevenfold higher than values achieved by SmF, showing that the expression of recombinant enzymes can be significantly improved by cultivation under SSF.

  12. Thermo-Chemical Conversion of Microwave Activated Biomass Mixtures

    NASA Astrophysics Data System (ADS)

    Barmina, I.; Kolmickovs, A.; Valdmanis, R.; Vostrikovs, S.; Zake, M.

    2018-05-01

    Thermo-chemical conversion of microwave activated wheat straw mixtures with wood or peat pellets is studied experimentally with the aim to provide more effective application of wheat straw for heat energy production. Microwave pre-processing of straw pellets is used to provide a partial decomposition of the main constituents of straw and to activate the thermo-chemical conversion of wheat straw mixtures with wood or peat pellets. The experimental study includes complex measurements of the elemental composition of biomass pellets (wheat straw, wood, peat), DTG analysis of their thermal degradation, FTIR analysis of the composition of combustible volatiles entering the combustor, the flame temperature, the heat output of the device and composition of the products by comparing these characteristics for mixtures with unprocessed and mw pre-treated straw pellets. The results of experimental study confirm that mw pre-processing of straw activates the thermal decomposition of mixtures providing enhanced formation of combustible volatiles. This leads to improvement of the combustion conditions in the flame reaction zone, completing thus the combustion of volatiles, increasing the flame temperature, the heat output from the device, the produced heat energy per mass of burned mixture and decreasing at the same time the mass fraction of unburned volatiles in the products.

  13. Chemical components and tyrosinase inhibitors from the twigs of Artocarpus heterophyllus.

    PubMed

    Zheng, Zong-Ping; Chen, Sibao; Wang, Shiyun; Wang, Xia-Chang; Cheng, Ka-Wing; Wu, Jia-Jun; Yang, Dajiang; Wang, Mingfu

    2009-08-12

    An HPLC method was developed and validated to compare the chemical profiles and tyrosinase inhibitors in the woods, twigs, roots, and leaves of Artocarpus heterophyllus . Five active tyrosinase inhibitors including dihydromorin, steppogenin, norartocarpetin, artocarpanone, and artocarpesin were used as marker compounds in this HPLC method. It was discovered that the chemical profiles of A. heterophyllus twigs and woods are quite different. Systematic chromatographic methods were further applied to purify the chemicals in the twigs of A. heterophyllus. Four new phenolic compounds, including one isoprenylated 2-arylbenzofuran derivative, artoheterophyllin A (1), and three isoprenylated flavonoids, artoheterophyllin B (2), artoheterophyllin C (3), and artoheterophyllin D (4), together with 16 known compounds, were isolated from the ethanol extract of the twigs of A. heterophyllus. The structures of compounds 1-4 were elucidated by spectroscopic analysis. However, the four new compounds did not show significant inhibitory activities against mushroom tyrosinase compared to kojic acid. It was found that similar compounds, such as norartocarpetin and artocarpesin in the twigs and woods of A. heterophyllus, contributed to their tyrosinase inhibitory activity.

  14. Nitrogen and Phosphorus Removal from Wastewater Treatment Plant Effluent via Bacterial Sulfate Reduction in an Anoxic Bioreactor Packed with Wood and Iron

    PubMed Central

    Yamashita, Takahiro; Yamamoto-Ikemoto, Ryoko

    2014-01-01

    We investigated the removal of nitrogen and phosphate from the effluent of a sewage treatment plant over a long-term operation in bioreactors packed with different combinations of wood and iron, with a trickling filter packed with foam ceramics for nitrification. The average nitrification rate in the trickling filter was 0.17 kg N/m3∙day and remained at 0.11 kg N/m3∙day even when the water temperature was below 15 °C. The denitrification and phosphate removal rates in the bioreactor packed with aspen wood and iron were higher than those in the bioreactor packed with cedar chips and iron. The bioreactor packed with aspen wood and iron continued to remove nitrate and phosphate for >1200 days of operation. The nitrate removal activity of a biofilm attached to the aspen wood from the bioreactor after 784 days of operation was 0.42 g NO3-N/kg dry weight wood∙ day. There was no increase in the amount of dissolved organic matter in the outflow from the bioreactors. PMID:25247426

  15. Vertical feed stick wood fuel burning furnace system

    DOEpatents

    Hill, Richard C.

    1982-01-01

    A stove or furnace for efficient combustion of wood fuel includes a vertical feed combustion chamber (15) for receiving and supporting wood fuel in a vertical attitude or stack. A major upper portion of the combustion chamber column comprises a water jacket (14) for coupling to a source of water or heat transfer fluid for convection circulation of the fluid. The locus (31) of wood fuel combustion is thereby confined to the refractory base of the combustion chamber. A flue gas propagation delay channel (34) extending laterally from the base of the chamber affords delayed travel time in a high temperature refractory environment sufficient to assure substantially complete combustion of the gaseous products of wood burning with forced air prior to extraction of heat in heat exchanger (16). Induced draft draws the fuel gas and air mixture laterally through the combustion chamber and refractory high temperature zone to the heat exchanger and flue. Also included are active sources of forced air and induced draft, multiple circuit couplings for the recovered heat, and construction features in the refractory material substructure and metal component superstructure.

  16. Technical change in forest sector models: the global forest products model approach

    Treesearch

    Joseph Buongiorno; Sushuai Zhu

    2015-01-01

    Technical change is developing rapidly in some parts of the forest sector, especially in the pulp and paper industry where wood fiber is being substituted by waste paper. In forest sector models, the processing of wood and other input into products is frequently represented by activity analysis (input–output). In this context, technical change translates in changes...

  17. Developing Evidence for Structural Approaches to Build a Culture of Health: A Perspective from the Robert Wood Johnson Foundation

    ERIC Educational Resources Information Center

    Mockenhaupt, Robin; Woodrum, Amy

    2015-01-01

    We believe that reframing the conversation to creating a culture around health rather than focusing on discrete actions or activities will capture national consciousness and enable us to make new progress as a nation. Thus, in 2014, the Robert Wood Johnson Foundation (RWJF) announced a new vision to help build a "Culture of Health" to…

  18. Enzymatic oxalic acid regulation correlated with wood degradation in four brown-rot fungi

    Treesearch

    Anne Christine Steenkjær Hastrup; Frederick Green III; Patricia K. Lebow; Bo Jensen

    2012-01-01

    Oxalic acid is a key component in the initiation of brown-rot decay and it has been suggested that it plays multiple roles during the degradation process. Oxalic acid is accumulated to varying degrees among brown-rot fungi; however, details on active regulation are scarce. The accumulation of oxalic acid was measured in this study from wood degraded by the four brown-...

  19. Sensory and Emotional Perception of Wooden Surfaces through Fingertip Touch

    PubMed Central

    Bhatta, Shiv R.; Tiippana, Kaisa; Vahtikari, Katja; Hughes, Mark; Kyttä, Marketta

    2017-01-01

    Previous studies on tactile experiences have investigated a wide range of material surfaces across various skin sites of the human body in self-touch or other touch modes. Here, we investigate whether the sensory and emotional aspects of touch are related when evaluating wooden surfaces using fingertips in the absence of other sensory modalities. Twenty participants evaluated eight different pine and oak wood surfaces, using sensory and emotional touch descriptors, through the lateral motion of active fingertip exploration. The data showed that natural and smooth wood surfaces were perceived more positively in emotional touch than coated surfaces. We highlight the importance of preserving the naturalness of the surface texture in the process of wood-surface treatment so as to improve positive touch experiences, as well as avoid negative ones. We argue that the results may offer possibilities in the design of wood-based interior products with a view to improving consumer touch experiences. PMID:28348541

  20. Application of hinokitiol potassium salt for wood preservative.

    PubMed

    Hu, Junyi; Shen, Yu; Pang, Song; Gao, Yun; Xiao, Guoyong; Li, Shujun; Xu, Yingqian

    2013-12-01

    The decay of wood and other cellulosic materials by fungi cause significant economic loss. The widely used chromated copper arsenate was prohibited for the environmental impact and safety of arsenic and chromium. It was found that natural product hinokitiol (HK) had fungicidal and insecticidal activities, and its toxicity was bearable for the environment. We described the practical synthesis of HK-K salt. According to the GB/T18261-2000 and LY/T1283-1998, wood preservative performance of HK-K salt was tested. The results showed that the best inhibitory concentration of HK-K salt was 50 mg/L, for which the prevention effectiveness on mold is better, the killed value is between 0 and 1, and the corrosion-resistant for wood-rotting fungi is grade A. Copyright © 2013 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.

  1. Peculiarities of the processes of hydration of binding substances in the arbolite mixture

    NASA Astrophysics Data System (ADS)

    Innokentieva, L. S.; Egorova, A. D.; Emelianova, Z. V.

    2017-09-01

    Cement and sand solution is traditionally used for production of wood concrete. But it is known that impact of water-soluble substances of wood on the hardening cement is shown in the stabilizing effect. The "Cement poisons" consisting generally of the HOCH carbohydrate groups, sedimented on a surface of particles of minerals of cement 3CaO.SiO2 (three-calcic silicate) and 3CaO.Al2O3 (three-calcic aluminate) form the thinnest covers which complicate the course of processes of hydration of cement. Plaster in comparison with cement is less sensitive to extractive substances of wood therefore their combination to wood (including waste of logging and a woodworking) both coniferous and deciduous species is allowed. Composite plaster binding with hongurin as active mineral additive agent are applied at selection of composition of arbolite, at the same time dependences of their physicomechanical properties on characteristics of filler are received.

  2. Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46.

    PubMed

    Balachandran, Manasi; Giannone, Richard J; Bemis, David A; Kania, Stephen A

    2017-01-01

    Protein A in Staphylococcus aureus is encoded by the spa (staphylococcal protein A) gene and binds to immunoglobulin (Ig). The S. aureus strain Wood 46 has been variously reported as protein A-deficient and/or spa negative and used as a control in animal models of staphylococcal infections. The results of this study indicate that Wood 46 has normal spa expression but transcribes very low levels of the srtA gene which encodes the sortase A (SrtA) enzyme. This is consistent with unique mutations in the srtA promoter. In this study, a low level of sortase A explains deficient anchoring of proteins with an LPXTG motif, such as protein A, fibrinogen-binding protein and fibronectin-binding proteins A and B on to the peptidoglycan cell wall. The activity of secreted protein A is an important consideration for use of Wood 46 in functional experiments and animal models.

  3. Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46

    PubMed Central

    Balachandran, Manasi; Giannone, Richard J.; Bemis, David A.

    2017-01-01

    Protein A in Staphylococcus aureus is encoded by the spa (staphylococcal protein A) gene and binds to immunoglobulin (Ig). The S. aureus strain Wood 46 has been variously reported as protein A-deficient and/or spa negative and used as a control in animal models of staphylococcal infections. The results of this study indicate that Wood 46 has normal spa expression but transcribes very low levels of the srtA gene which encodes the sortase A (SrtA) enzyme. This is consistent with unique mutations in the srtA promoter. In this study, a low level of sortase A explains deficient anchoring of proteins with an LPXTG motif, such as protein A, fibrinogen-binding protein and fibronectin-binding proteins A and B on to the peptidoglycan cell wall. The activity of secreted protein A is an important consideration for use of Wood 46 in functional experiments and animal models. PMID:28859130

  4. Amplicon-Based Sequencing of Soil Fungi from Wood Preservative Test Sites

    PubMed Central

    Kirker, Grant T.; Bishell, Amy B.; Jusino, Michelle A.; Palmer, Jonathan M.; Hickey, William J.; Lindner, Daniel L.

    2017-01-01

    Soil samples were collected from field sites in two AWPA (American Wood Protection Association) wood decay hazard zones in North America. Two field plots at each site were exposed to differing preservative chemistries via in-ground installations of treated wood stakes for approximately 50 years. The purpose of this study is to characterize soil fungal species and to determine if long term exposure to various wood preservatives impacts soil fungal community composition. Soil fungal communities were compared using amplicon-based DNA sequencing of the internal transcribed spacer 1 (ITS1) region of the rDNA array. Data show that soil fungal community composition differs significantly between the two sites and that long-term exposure to different preservative chemistries is correlated with different species composition of soil fungi. However, chemical analyses using ICP-OES found levels of select residual preservative actives (copper, chromium and arsenic) to be similar to naturally occurring levels in unexposed areas. A list of indicator species was compiled for each treatment-site combination; functional guild analyses indicate that long-term exposure to wood preservatives may have both detrimental and stimulatory effects on soil fungal species composition. Fungi with demonstrated capacity to degrade industrial pollutants were found to be highly correlated with areas that experienced long-term exposure to preservative testing. PMID:29093702

  5. Plasticity of maritime pine (Pinus pinaster) wood-forming tissues during a growing season.

    PubMed

    Paiva, J A P; Garnier-Géré, P H; Rodrigues, J C; Alves, A; Santos, S; Graça, J; Le Provost, G; Chaumeil, G; Da Silva-Perez, D; Bosc, A; Fevereiro, P; Plomion, C

    2008-01-01

    The seasonal effect is the most significant external source of variation affecting vascular cambial activity and the development of newly divided cells, and hence wood properties. Here, the effect of edapho-climatic conditions on the phenotypic and molecular plasticity of differentiating secondary xylem during a growing season was investigated. Wood-forming tissues of maritime pine (Pinus pinaster) were collected from the beginning to the end of the growing season in 2003. Data from examination of fibre morphology, Fourier-transform infrared spectroscopy (FTIR), analytical pyrolysis, and gas chromatography/mass spectrometry (GC/MS) were combined to characterize the samples. Strong variation was observed in response to changes in edapho-climatic conditions. A genomic approach was used to identify genes differentially expressed during this growing season. Out of 3512 studied genes, 19% showed a significant seasonal effect. These genes were clustered into five distinct groups, the largest two representing genes over-expressed in the early- or late-wood-forming tissues, respectively. The other three clusters were characterized by responses to specific edapho-climatic conditions. This work provides new insights into the plasticity of the molecular machinery involved in wood formation, and reveals candidate genes potentially responsible for the phenotypic differences found between early- and late-wood.

  6. The Ancient Wood of the Acqualadrone Rostrum: A Materials History Through GC-MS and Sulfur X-ray Absorption Spectroscopy

    PubMed Central

    Frank, Patrick; Caruso, Francesco; Caponetti, Eugenio

    2012-01-01

    In 2008 the rostrum from an ancient warship was recovered from the Mediterranean near Acqualadrone, Sicily. To establish its provenance and condition, samples of black and brown rostrum wood were examined using sulfur K-edge x-ray absorption spectroscopy (XAS) and GC-MS. GC-MS of pyrolytic volatiles yielded only guaiacyl derivatives, indicating construction from pinewood. A derivatized extract of black wood yielded forms of abietic acid and sandaracopimaric acid consistent with pine pitch waterproofing. Numerical fits to the sulfur K-edge XAS spectra showed that about 65% of the endogenous sulfur consisted of thiols and disulfides. Elemental sulfur was about 2% and 7% in black and brown wood, respectively, while pyritic sulfur was about 12% and 6%. About 2% of the sulfur in both wood types was modeled as trimethylsulfonium, possibly reflecting biogenic dimethylsulfonio-propionate. High valent sulfur was exclusively represented by sulfate esters, consistent with bacterial sulfotransferase activity. Traces of chloride were detected, but no free sulfate ion. In summary, the rostrum was manufactured of pine wood and subsequently waterproofed with pine pitch. The subsequent 2300 years included battle, foundering, and marine burial followed by anoxia, bacterial colonization, sulfate reduction, and mobilization of transition metals, which produced pyrite and copious appended sulfur functionality. PMID:22545724

  7. Embryo toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin to the wood duck (Aix sponsa)

    USGS Publications Warehouse

    Augspurger, T.P.; Tillitt, D.E.; Bursian, S.J.; Fitzgerald, S.D.; Hinton, D.E.; Di Giulio, R.T.

    2008-01-01

    We examined the sensitivity of the wood duck (Aix sponsa) embryo to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) by injecting the toxicant into their eggs. Six groups of wood duck eggs (n = 35 to 211 per trial) were injected with 0 to 4600 pg TCDD/g egg between 2003 and 2005. Injections were made into yolk prior to incubation, and eggs were subsequently incubated and assessed weekly for mortality. Significant TCDD-induced mortality was not observed through day 25 (90% of incubation). Liver, heart, eye, and brain histology were generally unremarkable. Hepatic ethoxyresorufin-O-deethylase activity, a biomarker of dioxin-like compound exposure, was induced by 12-fold in the 4600 pg/g treatment relative to controls. The median lethal dose for chicken (Gallus domesticus) eggs we dosed identically to wood duck eggs was about 100 pg/g, similar to other assessments of chickens. Among dioxin-like compound embryo lethality data for 15 avian genera, the wood duck 4600 pg/g no-observed-effect level ranks near the middle. Because no higher doses were tested, wood ducks may be like other waterfowl (order Anseriformes), which are comparatively tolerant to embryo mortality from polychlorinated dibenzo-p-dioxins and dibenzofurans when exposed by egg injection. ?? 2008 US Government.

  8. Evaluation of photo-mutagenicity and photo-cytotoxicity of food coloring agents.

    PubMed

    Arimoto-Kobayashi, Sakae; Machida, Masaki; Okamoto, Keinosuke; Yamaguchi, Akie

    2005-05-01

    Pigments extracted from natural products are widely used for food coloration in Japan. An investigation concerning the photo-mutagenicity and photo-carcinogenicity of frequently used colorants in Japan was performed. Colorants examined were from Laccifer lacca (lac-color), Coccus cacti (cochineal-color), Carthamus tinctorius (carthamus yellow), Gardenia augusta (gardenia yellow and gardenia blue), Monascus anka and Monascus purpureus (monascus red), the skin of Vitis vinifera and Vitis labrusca (grape-skin color), Tamarindus indica (tamarind brown) and Beta vulgaris (beet red). No significant increase in bacterial mutation was found when Salmonella typhimurium TA98, TA100 and TA102 were simultaneously treated with colorants and subjected to UVA irradiation for 30 min. When colorant solutions were subjected to UVA irradiation for 4 h, irradiated solutions containing lac-color became slightly mutagenic toward S.typhimurium TA98 without metabolic activation. A decrease in cell survival resulted when WTK-1 cells were subjected to UVA irradiation for 60 min in the presence of purpurin at 1 mg/ml. Delayed cytotoxicity was also observed following 24 h incubation in fresh medium of samples that were subjected to UVA irradiation for 60 min in the presence of colorant (carthamus yellow, grape-skin color, gardenia blue, cochineal-color, monascus red or purpurin).

  9. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Odelson, D.A.; Breznak, J.A.

    Crude extracts of the anaerobic, cellulolytic protozoan Trichomitopsis termopsidis possessed endo-..beta..-1,4-glucanase and cellobiase activities, as evidenced by hydrolytic action on carboxymethyl cellulose and cellobiose, respectively. Cell extracts also hydrolyzed microcrystalline cellulose. Hydrolysis of microcrystalline cellulose displayed optima at pH 5 and at 30 degrees C, and glucose was the sole product liberated. Cellulolytic activities of T. termopsidis appeared to be entirely cell associated. Hydrolytic activity was also detected against Douglas fir wood powder, xylan, starch, and protein, but not chitin. The importance of these enyzmes in the nutrition of T. termopsidis is discussed in terms of the natural habitat ofmore » this protozoan (the hindgut of wood-eating termites). 31 references.« less

  10. Processes and rates of sediment and wood accumulation in headwater streams of the Oregon Coast Range, USA

    USGS Publications Warehouse

    May, Christine L.; Gresswell, Robert E.

    2003-01-01

    Channels that have been scoured to bedrock by debris flows provide unique opportunities to calculate the rate of sediment and wood accumulation in low-order streams, to understand the temporal succession of channel morphology following disturbance, and to make inferences about processes associated with input and transport of sediment. Dendrochronology was used to estimate the time since the previous debris flow and the time since the last stand-replacement fire in unlogged basins in the central Coast Range of Oregon. Debris flow activity increased 42 per cent above the background rate in the decades immediately following the last wildfire. Changes in wood and sediment storage were quantified for 13 streams that ranged from 4 to 144 years since the previous debris flow. The volume of wood and sediment in the channel, and the length of channel with exposed bedrock, were strongly correlated with the time since the previous debris flow. Wood increased the storage capacity of the channel and trapped the majority of the sediment in these steep headwater streams. In the absence of wood, channels that have been scoured to bedrock by a debris flow may lack the capacity to store sediment and could persist in a bedrock state for an extended period of time. With an adequate supply of wood, low-order channels have the potential of storing large volumes of sediment in the interval between debris flows and can function as one of the dominant storage reservoirs for sediment in mountainous terrain.

  11. Potassium contents of northeastern Thai foods.

    PubMed

    Sriboonlue, P; Prasongwatana, V; Suwantrai, S; Bovornpadungkitti, S; Tungsanga, K; Tosukhowong, P

    1998-08-01

    From our previous nutritional assessment, low potassium (K) intake among northeastern Thai males has been clearly demonstrated. This prompted us to undertake a survey of the K content of local foods. Food samples comprised of 57 animal and 142 plant products which were collected from various places in the northeast of Thailand. The dry ashing method was used to prepare the samples for K analysis using an atomic absorption spectrophotometer. Foods could be divided into 7 groups according to their K levels. Foods containing K > or = 1000 mg per 100 g fresh food were categorized in group 1. These were mainly foods in the legume group, i.e., soybean, cowpea and mungbean. While rice (polished) and rice products, the main staple, were in group 7, the lowest K group of less than 100 mg per 100 g fresh food. Comparison studies of the natural foods between those collected from the northeast and from the central regions of the country, and between the cooked foods purchased from the rural villages and from the urban areas of Khon Kaen municipality, showed that, for most food items, the K content was similar wherever it came from. However, when the K content in various parts or in different stages of growth of the same kind of plants or animals was compared, a great variation was clearly seen, for example, young tamarind leaves contained K in group 6 whereas ripe tamarind fruit contained K in group 1. According to our food consumption data, the analysis of food components of 48 meals taken during the hot season by 13 rural volunteers revealed that food items eaten with the highest frequencies and in the largest amount were those in the low K food groups, i.e., glutinous rice (group 7) and green papaya (group 6). Our results suggest that the low K intake of these northeast rural Thai people is not due to a low K content of foods in this region, but rather that their food habits and low socioeconomic status restricts consumption of those food items with higher K contents.

  12. A comparison of salivary pH in sympatric wild lemurs (Lemur catta and Propithecus verreauxi) at Beza Mahafaly Special Reserve, Madagascar.

    PubMed

    Cuozzo, Frank P; Sauther, Michelle L; Yamashita, Nayuta; Lawler, Richard R; Brockman, Diane K; Godfrey, Laurie R; Gould, Lisa; Youssouf, Ibrahim Antho Jacky; Lent, Cheryl; Ratsirarson, Joelisoa; Richard, Alison F; Scott, Jessica R; Sussman, Robert W; Villers, Lynne M; Weber, Martha A; Willis, George

    2008-04-01

    Chemical deterioration of teeth is common among modern humans, and has been suggested for some extinct primates. Dental erosion caused by acidic foods may also obscure microwear signals of mechanical food properties. Ring-tailed lemurs at the Beza Mahafaly Special Reserve (BMSR), Madagascar, display frequent severe tooth wear and subsequent tooth loss. In contrast, sympatric Verreaux's sifaka display far less tooth wear and infrequent tooth loss, despite both species regularly consuming acidic tamarind fruit. We investigated the potential impact of dietary acidity on tooth wear, collecting data on salivary pH from both species, as well as salivary pH from ring-tailed lemurs at Tsimanampesotse National Park, Madagascar. We also collected salivary pH data from ring-tailed lemurs at the Indianapolis Zoo, none of which had eaten for at least 12 hr before data collection. Mean salivary pH for the BMSR ring-tailed lemurs (8.098, n=41, SD=0.550) was significantly more alkaline than Verreaux's sifaka (7.481, n=26, SD=0.458). The mean salivary pH of BMSR (8.098) and Tsimanampesotse (8.080, n=25, SD=0.746) ring-tailed lemurs did not differ significantly. Salivary pH for the Indianapolis Zoo sample (8.125, n=16, SD=0.289) did not differ significantly from either the BMSR or Tsimanampesotse ring-tailed lemurs, but was significantly more alkaline than the BMSR Verreaux's sifaka sample. Regardless of the time between feeding and collection of pH data (from several minutes to nearly 1 hr), salivary pH for each wild lemur was above the "critical" pH of 5.5, below which enamel demineralization occurs. Thus, the high pH of lemur saliva suggests a strong buffering capacity, indicating the impact of acidic foods on dental wear is short-lived, likely having a limited effect. However, tannins in tamarind fruit may increase friction between teeth, thereby increasing attrition and wear in lemurs. These data also suggest that salivary pH varies between lemur species, corresponding to broad dietary categories.

  13. LC-MS/MS Tandem Mass Spectrometry for Analysis of Phenolic Compounds and Pentacyclic Triterpenes in Antifungal Extracts of Terminalia brownii (Fresen)

    PubMed Central

    Salih, Enass Y. A.; Fyhrquist, Pia; Abdalla, Ashraf M. A.; Abdelgadir, Abdelazim Y.; Kanninen, Markku; Sipi, Marketta; Luukkanen, Olavi; Fahmi, Mustafa K. M.; Elamin, Mai H.; Ali, Hiba A.

    2017-01-01

    Decoctions and macerations of the stem bark and wood of Terminalia brownii Fresen. are used in traditional medicine for fungal infections and as fungicides on field crops and in traditional granaries in Sudan. In addition, T. brownii water extracts are commonly used as sprays for protecting wooden houses and furniture. Therefore, using agar disc diffusion and macrodilution methods, eight extracts of various polarities from the stem wood and bark were screened for their growth-inhibitory effects against filamentous fungi commonly causing fruit, vegetable, grain and wood decay, as well as infections in the immunocompromised host. Ethyl acetate extracts of the stem wood and bark gave the best antifungal activities, with MIC values of 250 µg/mL against Nattrassia mangiferae and Fusarium verticillioides, and 500 µg/mL against Aspergillus niger and Aspergillus flavus. Aqueous extracts gave almost as potent effects as the ethyl acetate extracts against the Aspergillus and Fusarium strains, and were slightly more active than the ethyl acetate extracts against Nattrassia mangiferae. Thin layer chromatography, RP-HPLC-DAD and tandem mass spectrometry (LC-MS/MS), were employed to identify the chemical constituents in the ethyl acetate fractions of the stem bark and wood. The stem bark and wood were found to have a similar qualitative composition of polyphenols and triterpenoids, but differed quantitatively from each other. The stilbene derivatives, cis- (3) and trans- resveratrol-3-O-β-galloylglucoside (4), were identified for the first time in T. brownii. Moreover, methyl-(S)-flavogallonate (5), quercetin-7-β-O-di-glucoside (8), quercetin-7-O-galloyl-glucoside (10), naringenin-4′-methoxy-7-pyranoside (7), 5,6-dihydroxy-3′,4′,7-tri-methoxy flavone (12), gallagic acid dilactone (terminalin) (6), a corilagin derivative (9) and two oleanane type triterpenoids (1) and (2) were characterized. The flavonoids, a corilagin derivative and terminalin, have not been identified before in T. brownii. We reported earlier on the occurrence of methyl-S-flavogallonate and its isomer in the roots of T. brownii, but this is the first report on their occurrence in the stem wood as well. Our results justify the traditional uses of macerations and decoctions of T. brownii stem wood and bark for crop and wood protection and demonstrate that standardized extracts could have uses for the eco-friendly control of plant pathogenic fungi in African agroforestry systems. Likewise, our results justify the traditional uses of these preparations for the treatment of skin infections caused by filamentous fungi. PMID:29236070

  14. Distinct Growth and Secretome Strategies for Two Taxonomically Divergent Brown Rot Fungi.

    PubMed

    Presley, Gerald N; Schilling, Jonathan S

    2017-04-01

    Brown rot fungi are wood-degrading fungi that employ both oxidative and hydrolytic mechanisms to degrade wood. Hydroxyl radicals that facilitate the oxidative component are powerful nonselective oxidants and are incompatible with hydrolytic enzymes unless they are spatially segregated in wood. Differential gene expression has been implicated in the segregation of these reactions in Postia placenta , but it is unclear if this two-step mechanism varies in other brown rot fungi with different traits and life history strategies that occupy different niches in nature. We employed proteomics to analyze a progression of wood decay on thin wafers, using brown rot fungi with significant taxonomic and niche distances: Serpula lacrymans (Boletales; "dry rot" lumber decay) and Gloeophyllum trabeum (order Gloeophyllales; slash, downed wood). Both fungi produced greater oxidoreductase diversity upon wood colonization and greater glycoside hydrolase activity later, consistent with a two-step mechanism. The two fungi invested very differently, however, in terms of growth (infrastructure) versus protein secretion (resource capture), with the ergosterol/extracted protein ratio being 7-fold higher with S. lacrymans than with G. trabeum In line with the native substrate associations of these fungi, hemicellulase-specific activities were dominated by mannanase in S. lacrymans and by xylanase in G. trabeum Consistent with previous observations, S. lacrymans did not produce glycoside hydrolase 6 (GH6) cellobiohydrolases (CBHs) in this study, despite taxonomically belonging to the order Boletales, which is distinguished among brown rot fungi by having CBH genes. This work suggests that distantly related brown rot fungi employ staggered mechanisms to degrade wood, but the underlying strategies vary among taxa. IMPORTANCE Wood-degrading fungi are important in forest nutrient cycling and offer promise in biotechnological applications. Brown rot fungi are unique among these fungi in that they use a nonenzymatic oxidative pretreatment before enzymatic carbohydrate hydrolysis, enabling selective removal of carbohydrates from lignin. This capacity has independently evolved multiple times, but it is unclear if different mechanisms underpin similar outcomes. Here, we grew fungi directionally on wood wafers and we found similar two-step mechanisms in taxonomically divergent brown rot fungi. The results, however, revealed strikingly different growth strategies, with S. lacrymans investing more in biomass production than secretion of proteins and G. trabeum showing the opposite pattern, with a high diversity of uncharacterized proteins. The "simplified" S. lacrymans secretomic system could help narrow gene targets central to oxidative brown rot pretreatments, and a comparison of its distinctions with G. trabeum and other brown rot fungi (e.g., Postia placenta ) might offer similar traction in noncatabolic genes. Copyright © 2017 American Society for Microbiology.

  15. The Genome of Ganderma lucidum Provide Insights into Triterpense Biosynthesis and Wood Degradation

    PubMed Central

    Huang, Zhuo; Zhang, Hong-Mei; Liu, Wei; Liu, Le; Ma, Junping; Xia, Zhilan; Chen, Yuxin; Chen, Yuewen; Wang, Depeng; Ni, Peixiang; Guo, An-Yuan; Xiong, Xingyao

    2012-01-01

    Background Ganoderma lucidum (Reishi or Ling Zhi) is one of the most famous Traditional Chinese Medicines and has been widely used in the treatment of various human diseases in Asia countries. It is also a fungus with strong wood degradation ability with potential in bioenergy production. However, genes, pathways and mechanisms of these functions are still unknown. Methodology/Principal Findings The genome of G. lucidum was sequenced and assembled into a 39.9 megabases (Mb) draft genome, which encoded 12,080 protein-coding genes and ∼83% of them were similar to public sequences. We performed comprehensive annotation for G. lucidum genes and made comparisons with genes in other fungi genomes. Genes in the biosynthesis of the main G. lucidum active ingredients, ganoderic acids (GAs), were characterized. Among the GAs synthases, we identified a fusion gene, the N and C terminal of which are homologous to two different enzymes. Moreover, the fusion gene was only found in basidiomycetes. As a white rot fungus with wood degradation ability, abundant carbohydrate-active enzymes and ligninolytic enzymes were identified in the G. lucidum genome and were compared with other fungi. Conclusions/Significance The genome sequence and well annotation of G. lucidum will provide new insights in function analyses including its medicinal mechanism. The characterization of genes in the triterpene biosynthesis and wood degradation will facilitate bio-engineering research in the production of its active ingredients and bioenergy. PMID:22567134

  16. Quantifying the role of woody debris in providing bioenergetically favorable habitat for juvenile salmon

    NASA Astrophysics Data System (ADS)

    Harrison, L.; Hafs, A. W.; Utz, R.; Dunne, T.

    2013-12-01

    The habitat complexity of a riverine ecosystem substantially influences aquatic communities, and especially the bioenergetics of drift feeding fish. We coupled hydrodynamic and bioenergetic models to assess the influence of habitat complexity, generated via large woody debris (LWD) additions, on juvenile Chinook salmon (Oncorhynchus tshawytscha) growth potential in a river that lacked large wood. Model simulations indicated that LWD diversified the flow field, creating pronounced velocity gradients, which enhanced fish feeding and resting activities at the micro-habitat (sub-meter) scale. Fluid drag created by individual wood structures was increased under higher wood loading rates, leading to a 5-19% reduction in the reach-averaged velocity. We found that wood loading was asymptotically related to the reach-scale growth potential, suggesting that the river became saturated with LWD and additional loading would produce minimal benefit. In our study reach, LWD additions could potentially quadruple the potential growth area available before that limit was reached. Wood depletion in the world's rivers has been widely documented, leading to widespread attempts by river managers to reverse this trend by adding wood to simplified aquatic habitats, though systematic prediction of the effects of wood on fish growth has not been previously accomplished. We offer a quantitative, theory-based approach for assessing the role of wood on habitat potential as it affects fish growth at the micro-habitat and reach-scales. Fig. 1. Predicted flow field and salmon growth potential maps produced from model simulations with no woody debris (Graphs A and D), a low density (Graphs B and E), and a high density (Graphs C and E) of woody debris.

  17. An evaluation of three wood shred blends for post-fire erosion control using indoor simulated rain events on small plots

    Treesearch

    R. B. Foltz; N. S. Wagenbrenner

    2010-01-01

    The assessment teams who make post-fire stabilization and treatment decisions are under pressure to employ more effective and economic post-fire treatments, as wild fire activity and severity has increased in recent years across the western United States. Use of forest-native wood-based materials for hillslope mulching has been on the rise due to potential...

  18. Analysis of D-glucose metabolism of wood decay fungi using 13C-NMR and 13C-labeled substrates

    Treesearch

    Theodorus H. de Koker; Michael D. Mozuch; Philip J. Kersten

    2003-01-01

    D-Glucose metabolism is thought to be important during wood decay by fungi, not only for anabolic and catabolic purposes of central metabolism, but also as a potential source of peroxide required by extracellular peroxidases. There has been some confusion in the literature as to whether this peroxide-generating activity is of the glucose 1-oxidase or pyranose 2-oxidase...

  19. Post-fire erosion control mulches alter belowground processes and nitrate reductase activity of a perennial forb, heartleaf arnica (Arnica cordifolia)

    Treesearch

    Erin M. Berryman; Penelope Morgan; Peter R. Robichaud; Deborah Page-Dumroese

    2014-01-01

    Four years post-wildfire, we measured soil and plant properties on hillslopes treated with two different mulches (agricultural wheat straw and wood strands) and a control (unmulched, but burned). Soil total N was about 40% higher and microbial respiration of a standard wood substrate was nearly twice as high in the mulched plots compared to the unmulched plots. Greater...

  20. A quantitative study of the U.S. wood pallet industry based on supply chain management practices

    Treesearch

    Henry Quesada-Pineda; Scarlett Sanchez-Gomez; Philip A. Araman

    2012-01-01

    Pallets play an important role in the movement of goods from place to place. They are not only used in warehouses or distribution centers, but also in all those activities that require an efficient and effective method of transportation. To better understand business practices and external factors that impacts supply chain management (SCM), a survey of 1,500 U.S. wood...

  1. Golgi Enrichment and Proteomic Analysis of Developing Pinus radiata Xylem by Free-Flow Electrophoresis

    PubMed Central

    Macdonald, Lucy J.; Adams, Paul D.; Petzold, Christopher J.; Strabala, Timothy J.; Wagner, Armin; Heazlewood, Joshua L.

    2013-01-01

    Our understanding of the contribution of Golgi proteins to cell wall and wood formation in any woody plant species is limited. Currently, little Golgi proteomics data exists for wood-forming tissues. In this study, we attempted to address this issue by generating and analyzing Golgi-enriched membrane preparations from developing xylem of compression wood from the conifer Pinus radiata. Developing xylem samples from 3-year-old pine trees were harvested for this purpose at a time of active growth and subjected to a combination of density centrifugation followed by free flow electrophoresis, a surface charge separation technique used in the enrichment of Golgi membranes. This combination of techniques was successful in achieving an approximately 200-fold increase in the activity of the Golgi marker galactan synthase and represents a significant improvement for proteomic analyses of the Golgi from conifers. A total of thirty known Golgi proteins were identified by mass spectrometry including glycosyltransferases from gene families involved in glucomannan and glucuronoxylan biosynthesis. The free flow electrophoresis fractions of enriched Golgi were highly abundant in structural proteins (actin and tubulin) indicating a role for the cytoskeleton during compression wood formation. The mass spectrometry proteomics data associated with this study have been deposited to the ProteomeXchange with identifier PXD000557. PMID:24416096

  2. Poplar Wood Rays Are Involved in Seasonal Remodeling of Tree Physiology1[C][W

    PubMed Central

    Larisch, Christina; Dittrich, Marcus; Wildhagen, Henning; Lautner, Silke; Fromm, Jörg; Polle, Andrea; Hedrich, Rainer; Rennenberg, Heinz; Müller, Tobias; Ache, Peter

    2012-01-01

    Understanding seasonality and longevity is a major challenge in tree biology. In woody species, growth phases and dormancy follow one another consecutively. In the oldest living individuals, the annual cycle may run for more than 1,000 years. So far, however, not much is known about the processes triggering reactivation from dormancy. In this study, we focused on wood rays, which are known to play an important role in tree development. The transition phase from dormancy to flowering in early spring was compared with the phase of active growth in summer. Rays from wood samples of poplar (Populus × canescens) were enriched by laser microdissection, and transcripts were monitored by poplar whole-genome microarrays. The resulting seasonally varying complex expression and metabolite patterns were subjected to pathway analyses. In February, the metabolic pathways related to flower induction were high, indicating that reactivation from dormancy was already taking place at this time of the year. In July, the pathways related to active growth, like lignin biosynthesis, nitrogen assimilation, and defense, were enriched. Based on “marker” genes identified in our pathway analyses, we were able to validate periodical changes in wood samples by quantitative polymerase chain reaction. These studies, and the resulting ray database, provide new insights into the steps underlying the seasonality of poplar trees. PMID:22992511

  3. Ultra-high performance liquid chromatography coupled to mass spectrometry applied to the identification of valuable phenolic compounds from Eucalyptus wood.

    PubMed

    Santos, Sónia A O; Vilela, Carla; Freire, Carmen S R; Neto, Carlos Pascoal; Silvestre, Armando J D

    2013-11-01

    Ultra-high performance liquid chromatography (UHPLC) was applied for the first time in the analysis of wood extracts. The potential of this technique coupled to ion trap mass spectrometry in the rapid and effective detection and identification of bioactive components in complex vegetal samples was demonstrated. Several dozens of compounds were detected in less than 30min of analysis time, corresponding to more than 3-fold reduction in time, when compared to conventional HPLC analysis of similar extracts. The phenolic chemical composition of Eucalyptus grandis, Eucalyptus urograndis (E. grandis×E. urophylla) and Eucalyptus maidenii wood extracts was assessed for the first time, with the identification of 51 phenolic compounds in the three wood extracts. Twenty of these compounds are reported for the first time as Eucalyptus genus components. Ellagic acid and ellagic acid-pentoside are the major components in all extracts, followed by gallic and quinic acids in E. grandis and E. urograndis and ellagic acid-pentoside isomer, isorhamnetin-hexoside and gallic acid in E. maidenii. The antioxidant scavenging activity of the extracts was evaluated, with E. grandis wood extract showing the lowest IC50 value. Moreover, the antioxidant activity of these extracts was higher than that of the commercial antioxidant BHT and of those of the corresponding bark extracts. These results, together with the phenolic content values, open good perspectives for the exploitation of these renewable resources as a source of valuable phenolic compounds. Copyright © 2013 Elsevier B.V. All rights reserved.

  4. Plastic Response of Tracheids in Pinus pinaster in a Water-Limited Environment: Adjusting Lumen Size instead of Wall Thickness

    PubMed Central

    Carvalho, Ana; Nabais, Cristina; Vieira, Joana; Rossi, Sergio; Campelo, Filipe

    2015-01-01

    The formation of wood results from cambial activity and its anatomical properties reflect the variability of environmental conditions during the growing season. Recently, it was found that wood density variations in conifers growing under cold-limited environment result from the adjustment of cell wall thickness (CWT) to temperature. Additionally, it is known that intra-annual density fluctuations (IADFs) are formed in response to precipitation after the summer drought. Although IADFs are frequent in Mediterranean conifers no study has yet been conducted to determine if these structures result from the adjustment of lumen diameter (LD) or CWT to soil water availability. Our main objective is to investigate the intra-ring variation of wood anatomical features (LD and CWT) in Pinus pinaster Ait. growing under a water-limited environment. We compared the tracheidograms of LD and CWT for the years 2010–2013 in P. pinaster growing in the west coast of Portugal. Our results suggest a close association between LD and soil moisture content along the growing season, reinforcing the role of water availability in determining tracheid size. Compared with CWT, LD showed a higher intra- and inter-annual variability suggesting its strong adjustment value to variations in water availability. The formation of a latewood IADF appears to be predisposed by higher rates of cell production in spring and triggered by early autumn precipitation. Our findings reinforce the crucial role of water availability on cambial activity and wood formation in Mediterranean conifers, and emphasize the high plasticity of wood anatomical features under Mediterranean climate. PMID:26305893

  5. Plastic Response of Tracheids in Pinus pinaster in a Water-Limited Environment: Adjusting Lumen Size instead of Wall Thickness.

    PubMed

    Carvalho, Ana; Nabais, Cristina; Vieira, Joana; Rossi, Sergio; Campelo, Filipe

    2015-01-01

    The formation of wood results from cambial activity and its anatomical properties reflect the variability of environmental conditions during the growing season. Recently, it was found that wood density variations in conifers growing under cold-limited environment result from the adjustment of cell wall thickness (CWT) to temperature. Additionally, it is known that intra-annual density fluctuations (IADFs) are formed in response to precipitation after the summer drought. Although IADFs are frequent in Mediterranean conifers no study has yet been conducted to determine if these structures result from the adjustment of lumen diameter (LD) or CWT to soil water availability. Our main objective is to investigate the intra-ring variation of wood anatomical features (LD and CWT) in Pinus pinaster Ait. growing under a water-limited environment. We compared the tracheidograms of LD and CWT for the years 2010-2013 in P. pinaster growing in the west coast of Portugal. Our results suggest a close association between LD and soil moisture content along the growing season, reinforcing the role of water availability in determining tracheid size. Compared with CWT, LD showed a higher intra- and inter-annual variability suggesting its strong adjustment value to variations in water availability. The formation of a latewood IADF appears to be predisposed by higher rates of cell production in spring and triggered by early autumn precipitation. Our findings reinforce the crucial role of water availability on cambial activity and wood formation in Mediterranean conifers, and emphasize the high plasticity of wood anatomical features under Mediterranean climate.

  6. Aquatic community responses to salmon carcass analog and wood bundle additions in restored floodplain habitats in an Alaskan stream

    USGS Publications Warehouse

    Martin, Aaron E.; Wipfli, Mark S.; Spangler, Robert E.

    2010-01-01

    Land use activities often directly and indirectly limit the capacity of freshwater habitats to produce fish. Consequently, habitat creation and enhancement actions are often undertaken to increase the quantity and quality of resources available to aquatic communities within these impaired systems, with the intent to increase fish production. The objectives of this study were to (1) determine whether aquatic community colonization and development could be accelerated through additions of woody debris bundles and marine-derived nutrients (via salmon carcass analog pellets) and (2) measure how aquatic communities (biofilm, invertebrates, and fish) respond to these additions after the creation of off-channel (alcove) fish habitat in a stream in south-central Alaska. Biofilm, invertebrates, and juvenile coho salmon Oncorhynchus kisutch were sampled in four treatments (control, wood, analog, and analog plus wood). Biofilm chlorophyll-aconcentrations were 4–10 times higher in analog-enriched treatments than in the control and wood treatments. No treatment effects were detected in benthic invertebrate density; however, treatment differences were detected in coho salmon diets, with nearly twice the amount of invertebrate abundance and biomass (primarily various dipteran, ephemeropteran, and plecopteran larvae) in the analog and analog plus wood treatments compared with the control and wood treatments. Juvenile coho salmon density and biomass were significantly higher in the wood treatment than in the analog plus wood treatment, and fish in the control showed possible signs of density-dependent limitation. Further, body condition of juvenile coho salmon was highest in the two analog-enriched treatments at the end of the study; juveniles in these habitats showed nearly two times the condition increase of fish inhabiting the control and wood treatment alcoves. These results demonstrate that the combination of salmon carcass analog and woody debris bundle additions aids in the short-term development of aquatic communities in newly created off-channel habitats, providing a boost in limited resources such as food and shelter.

  7. Reducing indoor air pollutants with air filtration units in wood stove homes.

    PubMed

    McNamara, Marcy L; Thornburg, Jonathon; Semmens, Erin O; Ward, Tony J; Noonan, Curtis W

    2017-08-15

    Biomass burning has been shown to be a major source of poor indoor air quality (IAQ) in developing and higher income countries across the world. Specifically, wood burning for cooking and heating contributes to high indoor concentrations of fine (particles with aerodynamic diameters<2.5μm; PM 2.5 ) and coarse (particles with aerodynamic diameters <10μm and >2.5μm; PMc) particulate matter. Endotoxin, predominantly found within the coarse fraction of airborne particulate matter, is associated with proinflammatory effects and adverse outcomes among susceptible populations. The aim of this study was to assess the efficacy of air filter interventions in reducing indoor PM 2.5 , PMc, and PMc-associated endotoxin concentrations in homes using a wood stove for primary heating. Homes (n=48) were randomized to receive in-room air filtration units with either a high efficiency filter (i.e. active) or a lower efficiency fiberglass filter (i.e., placebo). The active filter intervention showed a 66% reduction in indoor PM 2.5 concentrations (95% CI: 42.2% to 79.7% reduction) relative to the placebo intervention. Both the active and the placebo filters were effective in substantially reducing indoor concentrations of PMc (63.3% and 40.6% average reduction for active and placebo filters, respectively) and PMc-associated endotoxin concentrations (91.8% and 80.4% average reductions, respectively). These findings support the use of high efficiency air filtration units for reducing indoor PM 2.5 in homes using a wood stove for primary heating. We also discovered that using lower efficiency, lower cost filter alternatives can be effective for reducing PMc and airborne endotoxin in homes burning biomass fuel. Copyright © 2017. Published by Elsevier B.V.

  8. Emission of polycyclic aromatic hydrocarbons, toxicity, and mutagenicity from domestic cooking using sawdust briquettes, wood, and kerosene.

    PubMed

    Kim, OanhNguyenThi; Nghiem, Le Hoang; Phyu, Yin Latt

    2002-03-01

    Smoke samples, in both gas and particulate matter (PM) phases, of the three domestic stoves were collected using U.S. EPA modified method 5 and were analyzed for 17 PAH (HPLC-UV), acute toxicity (Microtox test), and mutagenicity (Amestest). The gas phase of smoke contributed > or = 95% of 17 PAH, > or = 96% of toxicity, and > or = 60% of mutagenicity. The highest emission factor of 17 PAH was from sawdust briquettes (260 mg/kg), but the highest emission of 11 genotoxic PAH was from kerosene (28 mg/kg). PM samples of kerosene smoke were not toxic. The total toxicity emission factor was the highest from sawdust, followed by kerosene and wood fuel. Smoke samples from the kerosene stove were not mutagenic. TA98 indicated the presence of both direct and indirect mutagenic activities in PM samples of sawdust and wood fuel but only direct mutagenic activities in the gas phase. TA100 detected only direct mutagenic activities in both PM and gas-phase samples. The higher mutagenicity emission factor was from wood fuel, 12 x 10(6) revertants/kg (TA100-S9) and 3.5 x 10(6) (TA98-S9), and lower from sawdust, 2.9 x 10(6) (TA100-S9) and 2.8 x 10(6) (TA98-S9). The low burning rate and high efficiency of a kerosene stove have resulted in the lowest PAH, toxicity, and mutagenicity emissions from daily cooking activities. The bioassays produced toxicity and mutagenicity results in correspondence with the PAH content of samples. The tests could be used for a quick assessment of potential health risks.

  9. Process-based control of HAPs emissions from drying wood flakes.

    PubMed

    Banerjee, Sujit; Pendyala, Krishna; Buchanan, Mike; Yang, Rallming; Abu-Daabes, Malyuba; Otwell, Lawrence P E

    2006-04-01

    Industrial wood flake drying generates methanol, formaldehyde, and other hazardous air pollutants (HAPs). A simple theoretical model shows that particles smaller than 400 microm will begin to thermally degrade and release disproportionately large quantities of HAPs. This is confirmed in full-scale practice where particles smaller than 500 microm show visible signs of charring. Laboratory measurement of the activation energy for the breakdown of wood tissue into methanol and formaldehyde led to a value of about 17 kcal/mol. The apparent activation energy measured in the field was higher. This result was obtained under nonisothermal conditions and is biased high by the fines fraction of the furnish, which is exposed to elevated temperatures. It is proposed that a combination of screening out the fines fraction smaller than 500 microm and reducing the dryer inlet temperature will substantially reduce emissions, possibly to the point where control devices can be downsized or eliminated. Our findings allow these HAPs reductions to be semiquantitatively estimated.

  10. Survey of subterranean termites (Isoptera: Rhinotermitidae) in a managed silvicultural plantation in Portugal, using a line-intersection method (LIS).

    PubMed

    Nobre, T; Nunes, L; Bignell, D E

    2009-02-01

    Subterranean termites (Reticulitermes grassei) were surveyed over successive seasons in a managed eucalyptus plantation in southeastern Portugal for 26 months. Termite activity in seven diameter categories of lying dead wood was investigated by a modified line intersection method (LIS). Each item sampled was inspected and assessed for termite attack and for general (i.e. fungal) decay status using standard protocols. Line intersection is quantitative to the extent that it can link foraging and decay parameters to woody biovolume. It was found that termites selected items with larger diameter, the observed trend showing an exponential character with greater termite attack as diameter increased. Attack by termites was positively associated with prior decay by fungi. A clear positive relationship was shown between rainfall and total woody biovolume containing live termites, underlining the importance of moisture for termite activity. Subterranean termites appeared to be important wood decomposers in the woodland studied, with an average of 30% of lying dead wood branches showing signs of termite attack.

  11. Soil microbial community response to land use change in an agricultural landscape of western Kenya.

    PubMed

    Bossio, D A; Girvan, M S; Verchot, L; Bullimore, J; Borelli, T; Albrecht, A; Scow, K M; Ball, A S; Pretty, J N; Osborn, A M

    2005-01-01

    Tropical agroecosystems are subject to degradation processes such as losses in soil carbon, nutrient depletion, and reduced water holding capacity that occur rapidly resulting in a reduction in soil fertility that can be difficult to reverse. In this research, a polyphasic methodology has been used to investigate changes in microbial community structure and function in a series of tropical soils in western Kenya. These soils have different land usage with both wooded and agricultural soils at Kakamega and Ochinga, whereas at Ochinga, Leuro, Teso, and Ugunja a replicated field experiment compared traditional continuous maize cropping against an improved N-fixing fallow system. For all sites, principal component analysis of 16S rRNA gene denaturing gradient gel electrophoresis (DGGE) profiles revealed that soil type was the key determinant of total bacterial community structure, with secondary variation found between wooded and agricultural soils. Similarly, phospholipid fatty acid (PLFA) analysis also separated wooded from agricultural soils, primarily on the basis of higher abundance of monounsaturated fatty acids, anteiso- and iso-branched fatty acids, and methyl-branched fatty acids in the wooded soils. At Kakamega and Ochinga wooded soils had between five 5 and 10-fold higher levels of soil carbon and microbial biomass carbon than agricultural soils from the same location, whereas total enzyme activities were also lower in the agricultural sites. Soils with woody vegetation had a lower percentage of phosphatase activity and higher cellulase and chitinase activities than the agricultural soils. BIOLOG analysis showed woodland soils to have the greatest substrate diversity. Throughout the study the two functional indicators (enzyme activity and BIOLOG), however, showed lower specificity with respect to soil type and land usage than did the compositional indicators (DGGE and PLFA). In the field experiment comparing two types of maize cropping, both the maize yields and total microbial biomass were found to increase with the fallow system. Moreover, 16S rRNA gene and PLFA analyses revealed shifts in the total microbial community in response to the different management regimes, indicating that deliberate management of soils can have considerable impact on microbial community structure and function in tropical soils.

  12. Malondialdehyde and superoxide dismutase correlate with FEV(1) in patients with COPD associated with wood smoke exposure and tobacco smoking.

    PubMed

    Montaño, Martha; Cisneros, José; Ramírez-Venegas, Alejandra; Pedraza-Chaverri, José; Mercado, Daniel; Ramos, Carlos; Sansores, Raul H

    2010-08-01

    Tobacco smoking is the primary risk factor for chronic obstructive pulmonary disease (COPD). However, recent epidemiological studies have established domestic exposure to wood smoke and other biomass fuels as additional important risk factors, characteristic in developing countries. Oxidative stress is one of the mechanisms concerned with pathogenesis of COPD. However, the molecular mechanisms involved in the onset and progress of COPD associated with biomass and specifically that derived from wood smoke exposure remain unknown. We analyzed the relationship between forced expiratory volume in first second (FEV(1)) with plasma malondialdehyde (MDA) concentration and activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione-S-transferase (GST) in COPD patients associated with wood smoke (WSG; n = 30), tobacco smoking (TSG; n = 30), and healthy control subjects (HCG; n = 30). Differences between FEV(1) from WSG and TSG (58 +/- 22% and 51 +/- 24%, respectively) with HCG (100 +/- 6%) were observed (P < 0.01). Plasma MDA concentration was higher in both WSG and TSG (1.87 +/- 0.81 and 1.68 +/- 0.82 nmol/mL, respectively) compared with HCG (0.42 +/- 0.17 nmol/mL; P < 0.01). SOD activity showed a significant increase in both WSG and TSG (0.36 +/- 0.12 and 0.37 +/- 0.13 U/mL) compared with HCG (0.19 +/- 0.04 U/mL; P < 0.01). No differences were shown regarding GPx, GR, and GST activities between COPD and control groups. Inverse correlations were founded between MDA and SOD with FEV(1) in both COPD patients and control subjects (P < 0.001). These results indicate a role for oxidative stress in COPD associated with wood smoke similar to that observed with tobacco smoking in subjects who ceased at least 10 years previous to this study.

  13. Distinctive exotic flavor and aroma compounds of some exotic tropical fruits and berries: a review.

    PubMed

    Lasekan, Ola; Abbas, Kassim A

    2012-01-01

    The characteristic flavor of exotic tropical fruits is one of their most attractive attributes to consumers. In this article, the enormous diversity of exotic fruit flavors is reviewed. Classifying some of the exotic fruits into two classes on the basis of whether esters or terpenes predominate in the aroma was also attempted. Indeed, as far as exotic tropical fruits are concerned, the majority of fruits have terpenes predominating in their aroma profile. Some of the fruits in this group are the Amazonian fruits such as pitanga, umbu-caja, camu-camu, garcinia, and bacuri. The ester group is made up of rambutan, durians, star fruit, snake fruit, acerola, tamarind, sapodilla, genipap, soursop, cashew, melon, jackfruit, and cupuacu respectively. Also, the role of sulphur-volatiles in some of the exotic fruits is detailed.

  14. Removal of hexavalent chromium by biosorption process in rotating packed bed.

    PubMed

    Panda, M; Bhowal, A; Datta, S

    2011-10-01

    Removal of hexavalent chromium ions from an aqueous solution by crude tamarind (Tamarindus indica) fruit shell was examined in a rotating packed bed contactor by continuously recirculating a given volume of solution through the bed. Reduction of Cr(VI) to Cr(III) within the biosorbent appeared to be the removal mechanism. Depletion rate of Cr(VI) from, and release of reduced Cr(III) ions into the aqueous phase, was influenced by mass transfer resistance besides pH and packing depth. A mathematical model considering the reduction reaction to be irreversible and incorporating intraparticle and external phase mass transfer resistances represented the experimental data adequately. The study indicated that the limitations of fixed bed contactor operating under terrestrial gravity in intensifying mass transfer rates for this system can be overcome with rotating packed bed due to liquid flow under centrifugal acceleration.

  15. Sensory-directed identification of taste-active ellagitannins in American (Quercus alba L.) and European oak wood (Quercus robur L.) and quantitative analysis in bourbon whiskey and oak-matured red wines.

    PubMed

    Glabasnia, Arne; Hofmann, Thomas

    2006-05-03

    Aimed at increasing our knowledge on the sensory-active nonvolatiles migrating from oak wood into alcoholic beverages upon cooperaging, an aqueous ethanolic extract prepared from oak wood chips (Quercus alba L.) was screened for its key taste compounds by application of the taste dilution analysis. Purification of the compounds perceived with the highest sensory impacts, followed by liquid chromatography/mass spectrometry as well as one-dimensional and two-dimensional NMR experiments, revealed the ellagitannins vescalagin, castalagin, and grandinin, the roburins A-E, and 33-deoxy-33-carboxyvescalagin as the key molecules imparting an astringent oral sensation. To the best of our knowledge, 33-deoxy-33-carboxyvescalagin has as yet not been reported as a phytochemical in Q. alba L. In addition, the sensory activity of these ellagitannins was determined for the first time on the basis of their human threshold concentrations and dose/response functions. Furthermore, the ellagitannins have been quantitatively determined in extracts prepared from Q. alba L. and Quercus robur L., respectively, as well as in bourbon whiskey and oak-matured red wines, and the sensory contribution of the individual compounds has been evaluated for the first time on the basis of dose/activity considerations.

  16. Stimulation of pulmonary rapidly adapting receptors by inhaled wood smoke in rats

    PubMed Central

    Lai, C J; Kou, Y R

    1998-01-01

    The stimulation of pulmonary rapidly adapting receptors (RARs) by wood smoke was investigated. Impulses from seventy RARs were recorded in fifty-nine anaesthetized, open-chest and artificially ventilated rats; responses to delivery of 6 ml of wood smoke into the lungs were studied in sixty-one receptors whereas responses to histamine (10 or 100 μg kg−1, i.v.) were studied in the other nine. Delivery of wood smoke stimulated fifty-two of the sixty-one RARs studied. When stimulated, an intense burst of discharge was evoked within 1 or 2 s of smoke delivery. This increased activity quickly peaked in 1-3 s (Δ= 15.8 ± 1.6 impulses s−1; n = 61; mean ± s.e.m.), then declined and yet remained at a level higher than the baseline activity. The mean duration of the stimulation was 25.1 ± 2.7 s. In contrast, smoke delivery did not affect tracheal pressure. Peak responses of RARs to wood smoke were partially reduced by removal of smoke particulates and were largely attenuated by pretreatment with dimethylthiourea (DMTU, a hydroxyl radical scavenger), indomethacin (Indo, a cyclo-oxygenase inhibitor), or both DMTU and Indo (DMTU + Indo). Conversely, the peak responses of RARs were not significantly affected by pretreatment with isoprenaline (a bronchodilator) or vehicle for these chemicals. Additionally, pretreatment with DMTU, Indo, or DMTU + Indo did not significantly alter the RAR sensitivity to mechanical stimulation (constant-pressure lung inflation; 20 cmH2O). Of the nine RARs tested, six were stimulated by histamine and their sensitivity to this chemical irritant was not altered by pretreatment with DMTU + Indo. The results suggest that both the particulates and gas phases are responsible for, and both the hydroxyl radical and cyclo-oxygenase products are involved in, the stimulation of RARs by wood smoke. Furthermore, changes in lung mechanics following smoke delivery are not the cause of this afferent stimulation. PMID:9508820

  17. Salt stress induces the formation of a novel type of 'pressure wood' in two Populus species.

    PubMed

    Janz, Dennis; Lautner, Silke; Wildhagen, Henning; Behnke, Katja; Schnitzler, Jörg-Peter; Rennenberg, Heinz; Fromm, Jörg; Polle, Andrea

    2012-04-01

    • Salinity causes osmotic stress and limits biomass production of plants. The goal of this study was to investigate mechanisms underlying hydraulic adaptation to salinity. • Anatomical, ecophysiological and transcriptional responses to salinity were investigated in the xylem of a salt-sensitive (Populus × canescens) and a salt-tolerant species (Populus euphratica). • Moderate salt stress, which suppressed but did not abolish photosynthesis and radial growth in P. × canescens, resulted in hydraulic adaptation by increased vessel frequencies and decreased vessel lumina. Transcript abundances of a suite of genes (FLA, COB-like, BAM, XET, etc.) previously shown to be activated during tension wood formation, were collectively suppressed in developing xylem, whereas those for stress and defense-related genes increased. A subset of cell wall-related genes was also suppressed in salt-exposed P. euphratica, although this species largely excluded sodium and showed no anatomical alterations. Salt exposure influenced cell wall composition involving increases in the lignin : carbohydrate ratio in both species. • In conclusion, hydraulic stress adaptation involves cell wall modifications reciprocal to tension wood formation that result in the formation of a novel type of reaction wood in upright stems named 'pressure wood'. Our data suggest that transcriptional co-regulation of a core set of genes determines reaction wood composition. © 2011 The Authors. New Phytologist © 2011 New Phytologist Trust.

  18. Evidence of the late lignification of the G-layer in Simarouba tension wood, to assist understanding how non-G-layer species produce tensile stress.

    PubMed

    Roussel, Jean-Romain; Clair, Bruno

    2015-12-01

    To recover verticality after disturbance, angiosperm trees produce 'tension wood' allowing them to bend actively. The driving force of the tension has been shown to take place in the G-layer, a specific unlignified layer of the cell wall observed in most temperate species. However, in tropical rain forests, the G-layer is often absent and the mechanism generating the forces to reorient trees remains unclear. A study was carried out on tilted seedlings, saplings and adult Simarouba amara Aubl. trees-a species known to not produce a G-layer. Microscopic observations were done on sections of normal and tension wood after staining or observed under UV light to assess the presence/absence of lignin. We showed that S. amara produces a cell-wall layer with all of the characteristics typical of G-layers, but that this G-layer can be observed only as a temporary stage of the cell-wall development because it is masked by a late lignification. Being thin and lignified, tension wood fibres cannot be distinguished from normal wood fibres in the mature wood of adult trees. These observations indicate that the mechanism generating the high tensile stress in tension wood is likely to be the same as that in species with a typical G-layer and also in species where the G-layer cannot be observed in mature cells. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  19. 46 CFR 148.325 - Wood chips; wood pellets; wood pulp pellets.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 5 2014-10-01 2014-10-01 false Wood chips; wood pellets; wood pulp pellets. 148.325... § 148.325 Wood chips; wood pellets; wood pulp pellets. (a) This part applies to wood chips and wood pulp... cargo hold. (b) No person may enter a cargo hold containing wood chips, wood pellets, or wood pulp...

  20. 46 CFR 148.325 - Wood chips; wood pellets; wood pulp pellets.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 5 2012-10-01 2012-10-01 false Wood chips; wood pellets; wood pulp pellets. 148.325... § 148.325 Wood chips; wood pellets; wood pulp pellets. (a) This part applies to wood chips and wood pulp... cargo hold. (b) No person may enter a cargo hold containing wood chips, wood pellets, or wood pulp...

  1. 46 CFR 148.325 - Wood chips; wood pellets; wood pulp pellets.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 5 2013-10-01 2013-10-01 false Wood chips; wood pellets; wood pulp pellets. 148.325... § 148.325 Wood chips; wood pellets; wood pulp pellets. (a) This part applies to wood chips and wood pulp... cargo hold. (b) No person may enter a cargo hold containing wood chips, wood pellets, or wood pulp...

  2. 46 CFR 148.325 - Wood chips; wood pellets; wood pulp pellets.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 5 2011-10-01 2011-10-01 false Wood chips; wood pellets; wood pulp pellets. 148.325... § 148.325 Wood chips; wood pellets; wood pulp pellets. (a) This part applies to wood chips and wood pulp... cargo hold. (b) No person may enter a cargo hold containing wood chips, wood pellets, or wood pulp...

  3. Character of cellulase activity in the guts of flagellate-free termites with different feeding habits.

    PubMed

    Li, Zhi-Qiang; Liu, Bing-Rong; Zeng, Wen-Hui; Xiao, Wei-Liang; Li, Qiu-Jian; Zhong, Jun-Hong

    2013-01-01

    Cellulose digestion in termites (Isoptera) is highly important for ecological reasons and applications in biofuel conversion. The speciose Termitidae family has lost flagellates in the hindgut and developed diverse feeding habits. To address the response of cellulase activity to the differentiation of feeding habits, a comparative study of the activity and distribution of composite cellulases, endo-β-1,4-glucanase, and β-glucosidase was performed in seven common flagellate-free termites with three feeding habits: the humus-feeding termites Sinocapritermes mushae (Oshima et Maki), Malaysiocapritermes zhangfengensis Zhu, Yang et Huang and Pericapritermes jiangtsekiangensis (Kemner); the fungus-growing termites Macrotermes barneyi Light and Odontotermes formosanus (Shiraki); and the wood-feeding termites Nasutitermes parvonasutus (Shiraki) and Havilanditermes orthonasus (Tsai et Chen). The results showed that in diverse feeding groups, the wood-feeding group had the highest total composite cellulase and endo-β-1,4-glucanase activities, while the fungus-growing group had the highest β-glucosidase activity. In terms of the distribution of cellulase activity in the alimentary canals, the cellulase activities in wood-feeding termites were concentrated in the midgut, but there was no significant difference between all gut segments in humus-feeding termites. As for the fungus-growing termites, the main site of composite cellulase activity was in the midgut. The endo-β-1,4-glucanase activity was restricted to the midgut, but the primary site of β-glucosidase activity was in the foregut and the midgut (Mac. barneyi). The functions of the gut segments apparently differentiated between feeding groups. The results suggest that the differentiation of feeding habits in flagellate-free termites was characterized by the distribution of cellulases in the gut rather than by variations in cellulase activity.

  4. Character of Cellulase Activity in the Guts of Flagellate-Free Termites with Different Feeding Habits

    PubMed Central

    Li, Zhi-Qiang; Liu, Bing-Rong; Zeng, Wen-Hui; Xiao, Wei-Liang; Li, Qiu-Jian; Zhong, Jun-Hong

    2013-01-01

    Cellulose digestion in termites (Isoptera) is highly important for ecological reasons and applications in biofuel conversion. The speciose Termitidae family has lost flagellates in the hindgut and developed diverse feeding habits. To address the response of cellulase activity to the differentiation of feeding habits, a comparative study of the activity and distribution of composite cellulases, endo-β-1, 4-glucanase, and β-glucosidase was performed in seven common flagellate-free termites with three feeding habits: the humus-feeding termites Sinocapritermes mushae (Oshima et Maki), Malaysiocapritermes zhangfengensis Zhu, Yang et Huang and Pericapritermes jiangtsekiangensis (Kemner); the fungus-growing termites Macrotermes barneyi Light and Odontotermes formosanus (Shiraki); and the wood-feeding termites Nasutitermes parvonasutus (Shiraki) and Havilanditermes orthonasus (Tsai et Chen). The results showed that in diverse feeding groups, the wood-feeding group had the highest total composite cellulase and endo-β-1, 4-glucanase activities, while the fungus-growing group had the highest β-glucosidase activity. In terms of the distribution of cellulase activity in the alimentary canals, the cellulase activities in wood-feeding termites were concentrated in the midgut, but there was no significant difference between all gut segments in humus-feeding termites. As for the fungus-growing termites, the main site of composite cellulase activity was in the midgut. The endo-β-1, 4-glucanase activity was restricted to the midgut, but the primary site of β-glucosidase activity was in the foregut and the midgut (Mac. barneyi). The functions of the gut segments apparently differentiated between feeding groups. The results suggest that the differentiation of feeding habits in flagellate-free termites was characterized by the distribution of cellulases in the gut rather than by variations in cellulase activity. PMID:23895662

  5. An extended model of heartwood secondary metabolism informed by functional genomics.

    PubMed

    Celedon, Jose M; Bohlmann, Jörg

    2018-03-01

    The development of heartwood (HW) and the associated accumulation of secondary metabolites, which are also known as 'specialized metabolites' or 'extractives', is an important feature of tree biology. Heartwood development can affect tree health with broader implications for forest health. Heartwood development also defines a variety of wood quality traits that are important in the forest industry such as durability and colour of wood products. In the bioproducts industry, HW provides a source of high-value small molecules such as fragrances and antimicrobials. The HW properties of decay resistance in living trees, durability and colour of wood products, and small molecule bioproducts are largely defined by secondary metabolites, the biosynthesis of which appears to be activated during the onset of HW formation. Traditionally, it is thought that HW formation involves a spike in the activity of secondary metabolism in parenchyma cells in a transition zone between sapwood and HW, followed by programmed cell-death. The resulting HW tissue is thought to consist entirely of dead cells. Here, we discuss a variation of existing models of HW formation, based on the recent discovery of HW-specific transcriptome signatures of terpenoid biosynthesis in sandalwood (Santalum album L.) that invokes the activity of living cells in HW.

  6. Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Balachandran, Manasi; Giannone, Richard J.; Bemis, David A.

    Protein A in Staphylococcus aureus is encoded by the spa (staphylococcal protein A) gene and binds to immunoglobulin (Ig). The S. aureus strain Wood 46 has been variously reported as protein A-deficient and/or spa negative and used as a control in animal models of staphylococcal infections. The results of this study indicate that Wood 46 has normal spa expression but transcribes very low levels of the srtA gene which encodes the sortase A (SrtA) enzyme. This is consistent with unique mutations in the srtA promoter. In this study, a low level of sortase A explains deficient anchoring of proteins withmore » an LPXTG motif, such as protein A, fibrinogen-binding protein and fibronectin-binding proteins A and B on to the peptidoglycan cell wall. The activity of secreted protein A is an important consideration for use of Wood 46 in functional experiments and animal models.« less

  7. Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46

    DOE PAGES

    Balachandran, Manasi; Giannone, Richard J.; Bemis, David A.; ...

    2017-08-31

    Protein A in Staphylococcus aureus is encoded by the spa (staphylococcal protein A) gene and binds to immunoglobulin (Ig). The S. aureus strain Wood 46 has been variously reported as protein A-deficient and/or spa negative and used as a control in animal models of staphylococcal infections. The results of this study indicate that Wood 46 has normal spa expression but transcribes very low levels of the srtA gene which encodes the sortase A (SrtA) enzyme. This is consistent with unique mutations in the srtA promoter. In this study, a low level of sortase A explains deficient anchoring of proteins withmore » an LPXTG motif, such as protein A, fibrinogen-binding protein and fibronectin-binding proteins A and B on to the peptidoglycan cell wall. The activity of secreted protein A is an important consideration for use of Wood 46 in functional experiments and animal models.« less

  8. [Treatment of Urban Runoff Pollutants by a Multilayer Biofiltration System].

    PubMed

    Wang, Xiao-lu; Zuo, Jian-e; Gan, Li-li; Xing, Wei; Miao, Heng-feng; Ruan, Wen-quan

    2015-07-01

    In order to control the non-point source pollution from road runoff in Wuxi City effectively, a multilayer biofiltration system was designed to remove a variety of pollutants according to the characteristics of road runoff in Wuxi, and the experimental research was carried out to study the effect on rainwater pollution purification. The results show that the system has a good performance on removing suspended solids (SS), organic pollutant (COD), nitrogen and phosphorus: all types of multilayer biofiltration systems have a high removal rate for SS, which can reach 90%. The system with activated carbon (GAC) has higher removal rates for COD and phosphorus. The system with zeolite (ZFM) has a relatively better removal efficiency for nitrogen. The addition of wood chips in the system can significantly improve the system efficiency for nitrogen removal. Between the two configurations of layered and distributed wood chips, configurations of distributed wood chips reach higher COD, phosphorus and nitrogen pollutants removal efficiencies since they can reduce the release of wood chips dissolution.

  9. Implications from the Use of Non-timber Forest Products on the Consumption of Wood as a Fuel Source in Human-Dominated Semiarid Landscapes

    NASA Astrophysics Data System (ADS)

    Cavalcanti, Maria Clara B. T.; Ramos, Marcelo Alves; Araújo, Elcida L.; Albuquerque, Ulysses P.

    2015-08-01

    Little is known about what possible effects on wood resources might be caused by non-timber forest products (NTFPs). Here, we assessed the patterns of fuelwood consumption related to an NTFP ( Caryocar coriaceum) oil extraction and how this non-domestic activity can indirectly increase the use pressure on fuelwood species in a protected area, semiarid of Brazil. We conducted semi-structured interviews, in situ inventories, phytosociological surveys, and analyses of wood quality to identify the set of woody plants used in oil production. Householders use large volumes of dry wood and a set of woody species, which are highly exploited. Additionally, many preferred species have low fuel potential and suffer much use pressure. The best fuelwood species are underused, what requires management strategies to improve their potential as a source of energy. As a result, we suggest some conservation and management actions of fuelwood resources related to the use of NTFPs.

  10. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Olson, Ake; Aerts, Andrea; Asiegbu, Fred

    Parasitism and saprotrophic wood decay are two fungal strategies fundamental for succession and nutrient cycling in forest ecosystems. An opportunity to assess the trade-off between these strategies is provided by the forest pathogen and wood decayer Heterobasidion annosum sensu lato. We report the annotated genome sequence and transcript profiling, as well as the quantitative trait loci mapping, of one member of the species complex: H. irregulare. Quantitative trait loci critical for pathogenicity, and rich in transposable elements, orphan and secreted genes, were identified. A wide range of cellulose-degrading enzymes are expressed during wood decay. By contrast, pathogenic interaction between H.more » irregulare and pine engages fewer carbohydrate-active enzymes, but involves an increase in pectinolytic enzymes, transcription modules for oxidative stress and secondary metabolite production. Our results show a trade-off in terms of constrained carbohydrate decomposition and membrane transport capacity during interaction with living hosts. Our findings establish that saprotrophic wood decay and necrotrophic parasitism involve two distinct, yet overlapping, processes.« less

  11. Tracing nitrogen accumulation in decaying wood and examining its impact on wood decomposition rate

    NASA Astrophysics Data System (ADS)

    Rinne, Katja T.; Rajala, Tiina; Peltoniemi, Krista; Chen, Janet; Smolander, Aino; Mäkipää, Raisa

    2016-04-01

    Decomposition of dead wood, which is controlled primarily by fungi is important for ecosystem carbon cycle and has potentially a significant role in nitrogen fixation via diazotrophs. Nitrogen content has been found to increase with advancing wood decay in several studies; however, the importance of this increase to decay rate and the sources of external nitrogen remain unclear. Improved knowledge of the temporal dynamics of wood decomposition rate and nitrogen accumulation in wood as well as the drivers of the two processes would be important for carbon and nitrogen models dealing with ecosystem responses to climate change. To tackle these questions we applied several analytical methods on Norway spruce logs from Lapinjärvi, Finland. We incubated wood samples (density classes from I to V, n=49) in different temperatures (from 8.5oC to 41oC, n=7). After a common seven day pre-incubation period at 14.5oC, the bottles were incubated six days in their designated temperature prior to CO2 flux measurements with GC to determine the decomposition rate. N2 fixation was measured with acetylene reduction assay after further 48 hour incubation. In addition, fungal DNA, (MiSeq Illumina) δ15N and N% composition of wood for samples incubated at 14.5oC were determined. Radiocarbon method was applied to obtain age distribution for the density classes. The asymbiotic N2 fixation rate was clearly dependent on the stage of wood decay and increased from stage I to stage IV but was substantially reduced in stage V. CO2 production was highest in the intermediate decay stage (classes II-IV). Both N2 fixation and CO2 production were highly temperature sensitive having optima in temperature 25oC and 31oC, respectively. We calculated the variation of annual levels of respiration and N2 fixation per hectare for the study site, and used the latter data together with the 14C results to determine the amount of N2 accumulated in wood in time. The proportion of total nitrogen in wood originating from N2 increased from 0.4% (class I) to 22% (V). Despite significant N inputs, N2 fixation explained only 34%-57% of the increase in wood N content of classes III-V. The DNA results indicated that mycorrhizal colonization of wood could only partially explain the remaining increase in N content. However, majority of the samples contained one or more wood decomposing fungal species that have been reported to have the capability to produce rhizomorphs or mycelial cords used for scavenging nutrients from outside sources. Assuming that the remaining increase in N content was due to fungal activity, we modelled the δ15N variation of wood from class I to V and compared the modelled and measured δ15N values (r = 0.95, p<0.05). The increase in wood nitrogen content in time was observed to have a significant, positive impact on the respiration rate (I-IV: r = 0.57, p<0.01).

  12. Differential sensitivity to the antifouling chemical medetomidine between wood frog and American toad tadpoles with evidence for low-dose stimulation and high-dose inhibition of metamorphosis.

    PubMed

    Fong, Peter P; Lambert, Olivia J; Hoagland, Margot L; Kurtz, Emily R

    2018-05-05

    Antifouling chemicals are legacy contaminants in aquatic ecosystems. Previous experiments have shown that a 14-day exposure to the antifouling chemical medetomidine delays metamorphosis and reduces body mass in wood frog tadpoles. In the present study, we exposed wood frog tadpoles to medetomidine for 3, 7, and 10 days at 100 nM, 1 μM, and 10 μM. We also exposed American toad tadpoles to medetomidine for 3 days at four concentrations (10 nM, 100 nM, 1 μM, and 10 μM) in static renewal experiments. In each experiment, we measured growth, frequency and time to metamorphosis, and mass at metamorphosis. In both species, medetomidine significantly slowed development as measured by the Gosner stage. After 34 days in culture, wood frog tadpoles exposed to 1 and 10 μM medetomidine for as few as 3 days were significantly less developed compared to controls. Toads exposed to 1 μM medetomidine for 3 days were also significantly less developed on day 27, but by day 34, there was no difference from controls. For wood frogs, medetomidine significantly affected time to metamorphosis with a trend for tadpoles at lower concentrations metamorphosing sooner than those at higher concentrations. While medetomidine affected time to metamorphosis in wood frogs, it did not affect fresh mass, dry mass, or mortality compared to controls. Wood frog tadpoles that did not metamorphose after over 90 days in culture were more frequent in high-concentration groups than in the control. In toads, 10 μM medetomidine was 100% lethal within 23 days, but at the same concentration and duration, no wood frog tadpoles died. Lower concentrations were also significantly lethal to toads compared to controls, but tadpoles that survived in 10 and 100 nM metamorphosed sooner than those in 1 μM. Fresh mass of toad tadpoles exposed to 1 μm was significantly smaller at metamorphosis compared to that of controls. Medetomidine also affected the behavior of tadpoles. In toads, medetomidine significantly reduced both percent activity and startle response. In wood frogs, medetomidine significantly reduced percent activity, but increased startle response. We discuss our finding of low-dose stimulation and high-dose inhibition of different life history endpoints in terms of hormetic mechanisms. The differential sensitivity between species in terms of mortality, frequency of metamorphosis, and behavior highlights the potential negative environmental effects of medetomidine to amphibians.

  13. Highly porous activated carbons from resource-recovered Leucaena leucocephala wood as capacitive deionization electrodes.

    PubMed

    Hou, Chia-Hung; Liu, Nei-Ling; Hsi, Hsing-Cheng

    2015-12-01

    Highly porous activated carbons were resource-recovered from Leucaena leucocephala (Lam.) de Wit. wood through combined chemical and physical activation (i.e., KOH etching followed by CO2 activation). This invasive species, which has severely damaged the ecological economics of Taiwan, was used as the precursor for producing high-quality carbonaceous electrodes for capacitive deionization (CDI). Carbonization and activation conditions strongly influenced the structure of chars and activated carbons. The total surface area and pore volume of activated carbons increased with increasing KOH/char ratio and activation time. Overgasification induced a substantial amount of mesopores in the activated carbons. In addition, the electrochemical properties and CDI electrosorptive performance of the activated carbons were evaluated; cyclic voltammetry and galvanostatic charge/discharge measurements revealed a typical capacitive behavior and electrical double layer formation, confirming ion electrosorption in the porous structure. The activated-carbon electrode, which possessed high surface area and both mesopores and micropores, exhibited improved capacitor characteristics and high electrosorptive performance. Highly porous activated carbons derived from waste L. leucocephala were demonstrated to be suitable CDI electrode materials. Copyright © 2015 Elsevier Ltd. All rights reserved.

  14. In vitro immunotoxic and genotoxic activities of particles emitted from two different small-scale wood combustion appliances

    NASA Astrophysics Data System (ADS)

    Tapanainen, Maija; Jalava, Pasi I.; Mäki-Paakkanen, Jorma; Hakulinen, Pasi; Happo, Mikko S.; Lamberg, Heikki; Ruusunen, Jarno; Tissari, Jarkko; Nuutinen, Kati; Yli-Pirilä, Pasi; Hillamo, Risto; Salonen, Raimo O.; Jokiniemi, Jorma; Hirvonen, Maija-Riitta

    2011-12-01

    Residential wood combustion appliances emit large quantities of fine particles which are suspected to cause a substantial health burden worldwide. Wood combustion particles contain several potential health-damaging metals and carbon compounds such as polycyclic aromatic hydrocarbons (PAH), which may determine the toxic properties of the emitted particles. The aim of the present study was to characterize in vitro immunotoxicological and chemical properties of PM 1 ( Dp ≤ 1 μm) emitted from a pellet boiler and a conventional masonry heater. Mouse RAW264.7 macrophages were exposed for 24 h to different doses of the emission particles. Cytotoxicity, production of the proinflammatory cytokine TNF-α and the chemokine MIP-2, apoptosis and phases of the cell cycle as well as genotoxic activity were measured after the exposure. The type of wood combustion appliance had a significant effect on emissions and chemical composition of the particles. All the studied PM 1 samples induced cytotoxic, genotoxic and inflammatory responses in a dose-dependent manner. The particles emitted from the conventional masonry heater were 3-fold more potent inducers of programmed cell death and DNA damage than those emitted from the pellet boiler. Furthermore, the particulate samples that induced extensive DNA damage contained also large amounts of PAH compounds. Instead, significant differences between the studied appliances were not detected in measurements of inflammatory mediators, although the chemical composition of the combustion particles differed considerably from each other. In conclusion, the present results show that appliances representing different combustion technology have remarkable effects on physicochemical and associated toxicological and properties of wood combustion particles. The present data indicate that the particles emitted from incomplete combustion are toxicologically more potent than those emitted from more complete combustion processes.

  15. Summary of energy planning technical support to the Government of Liberia

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Samuels, G.

    1985-06-01

    Subsequent to a general assessment of energy options for Liberia, the principal activities of this program were: (1) an assessment of the economics of wood energy in Liberia; (2) a study of the potential for energy conservation in government buildings; (3) assistance in completing the 1982 Liberian energy balance; and (4) assistance in preparing the National Energy Plan. This report discusses the first three of these activities. A draft of the National Energy Plan was submitted in January 1985 to member agencies of the Liberian National Energy Committee for their review and comments. Liberia used the equivalent of 13.2 millionmore » barrels of crude oil in 1982- 67% from fuel wood, 4% from hydro, and 29% from imported petroleum. The wood was used almost entirely (approx. 99%) by the residential sector. Iron ore mining operations accounted for about 60% of domestic consumption of petroleum products. The transportation sector accounted for another 25%. The energy consumed by the agriculture and forestry sector was less than 2% of domestic consumption and was used primarily for operations of the large rubber plantations and timber concessions. Very little energy was used for food production. Significant energy savings in government buildings would require a major remodeling effort, including replacement of the louvered windows; extensive repairs to close large gaps around windows, air conditioners, and doors; and extensive caulking. The payback period from energy savings would be long. The assessment of the economics of wood energy indicates that wood can probably be delivered to a small rural power plant at costs that make this feedstock highly competitive for some and perhaps most of Liberia's rural electric stations.« less

  16. Defeating copper tolerance: an example of how “omics” research can accelerate discovery of new wood protection compounds

    Treesearch

    Juliet D. Tang; Tina Ciaramitaro; Darrel D. Nicholas; Maria Tomaso-Peterson; Susan V. Diehl

    2017-01-01

    Imagine if you could measure all the genes being expressed at any one time in an organism and you knew what all the genes did in a cell. The power of this knowledge would allow you to determine how organisms regulate gene activity to survive. This is the essence of how “omics” science accelerates biological understanding. From a wood protections standpoint,...

  17. BATTLE (Biomarker-based Approach of Targeted Therapy for Lung Cancer Elimination)

    DTIC Science & Technology

    2012-04-01

    Wistuba,Ignacio Ivan Woods ,Denise M Wu,Huakang Xue,Yuwen Yang,Bijun Yin,Ming Zhang,Guangcheng Zhang,Li Army...Oncol. 2009; 27: 271–8. 127 Ishii Y, Rex M, Scotting PJ et al. Region-specific expression of chicken Sox2 in the developing gut and lung epithelium... Wood ER, Shewchuk L, Hassel A, et al. Discovery of an in- hibitor of insulin-like growth factor 1 receptor activation: implications for cellular potency

  18. Pretreatment of eucalyptus wood chips for enzymatic saccharification using combined sulfuric acid-free ethanol cooking and ball milling.

    PubMed

    Teramoto, Yoshikuni; Tanaka, Noriko; Lee, Seung-Hwan; Endo, Takashi

    2008-01-01

    A combined sulfuric acid-free ethanol cooking and pulverization process was developed in order to achieve the complete saccharification of the cellulosic component of woody biomass, thereby avoiding the problems associated with the use of strong acid catalysts. Eucalyptus wood chips were used as a raw material and exposed to an ethanol/water/acetic acid mixed solvent in an autoclave. This process can cause the fibrillation of wood chips. During the process, the production of furfural due to an excessive degradation of polysaccharide components was extremely low and delignification was insignificant. Therefore, the cooking process is regarded not as a delignification but as an activation of the original wood. Subsequently, the activated solid products were pulverized by ball-milling in order to improve their enzymatic digestibility. Enzymatic hydrolysis experiments demonstrated that the conversion of the cellulosic components into glucose attained 100% under optimal conditions. Wide-angle X-ray diffractometry and particle size distribution analysis revealed that the scale affecting the improvement of enzymatic digestibility ranged from 10 nm to 1 microm. Field emission scanning electron microscopy depicted that the sulfuric acid-free ethanol cooking induced a pore formation by the removal of part of the lignin and hemicellulose fractions in the size range from a few of tens nanometers to several hundred nanometers. (c) 2007 Wiley Periodicals, Inc.

  19. Suppression of xylan endotransglycosylase PtxtXyn10A affects cellulose microfibril angle in secondary wall in aspen wood.

    PubMed

    Derba-Maceluch, Marta; Awano, Tatsuya; Takahashi, Junko; Lucenius, Jessica; Ratke, Christine; Kontro, Inkeri; Busse-Wicher, Marta; Kosik, Ondrej; Tanaka, Ryo; Winzéll, Anders; Kallas, Åsa; Leśniewska, Joanna; Berthold, Fredrik; Immerzeel, Peter; Teeri, Tuula T; Ezcurra, Ines; Dupree, Paul; Serimaa, Ritva; Mellerowicz, Ewa J

    2015-01-01

    Certain xylanases from family GH10 are highly expressed during secondary wall deposition, but their function is unknown. We carried out functional analyses of the secondary-wall specific PtxtXyn10A in hybrid aspen (Populus tremula × tremuloides). PtxtXyn10A function was analysed by expression studies, overexpression in Arabidopsis protoplasts and by downregulation in aspen. PtxtXyn10A overexpression in Arabidopsis protoplasts resulted in increased xylan endotransglycosylation rather than hydrolysis. In aspen, the enzyme was found to be proteolytically processed to a 68 kDa peptide and residing in cell walls. Its downregulation resulted in a corresponding decrease in xylan endotransglycosylase activity and no change in xylanase activity. This did not alter xylan molecular weight or its branching pattern but affected the cellulose-microfibril angle in wood fibres, increased primary growth (stem elongation, leaf formation and enlargement) and reduced the tendency to form tension wood. Transcriptomes of transgenic plants showed downregulation of tension wood related genes and changes in stress-responsive genes. The data indicate that PtxtXyn10A acts as a xylan endotransglycosylase and its main function is to release tensional stresses arising during secondary wall deposition. Furthermore, they suggest that regulation of stresses in secondary walls plays a vital role in plant development. © 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.

  20. A comparative study on the properties of graphene oxide and activated carbon based sustainable wood starch composites.

    PubMed

    Baishya, Prasanta; Maji, Tarun Kumar

    2018-08-01

    Activated carbon (AC) prepared from Jatropha curcas and graphene oxide (GO) were employed in the preparation of natural polymer based wood starch composites (WSC) through the solution blending technique using water as a solvent. In this study, methyl methacrylate (MMA) was grafted onto the starch polymer and this MMA grafted starch (MMA-g-starch) was cross-linked with the cheap soft wood flour using the citric acid as cross-linker and water as a solvent in the whole process. The prepared GO and AC were characterized through Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), thermogravimetric analysis (TGA) and Raman study. The interaction of GO and AC, with MMA-g-starch, citric acid and wood were studied by FTIR, XRD and SEM analysis. The GO and AC treated composites exhibited outstanding mechanical properties, thermal stability and fire resistance properties. The tensile strength of the composites increased by 178% and 200% with addition of 2 phr AC and GO respectively compared to untreated composites. A significant enhancement in water resistance properties of GO and AC treated composites was also attained. The study showed that the properties of the composites containing AC prepared from the seeds of Jatropha curcas was quite comparable with the composites reinforced with GO. Copyright © 2018 Elsevier B.V. All rights reserved.

  1. Nucleolus organizer regions and B-chromosomes of wood mice (mammalia, rodentia, Apodemus)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Boeskorov, G.G.; Kartavtseva, I.V.; Zagorodnyuk, I.V.

    1995-02-01

    Distribution of nucleolus organizer regions (NORs) in karyotypes was studied in 10 species of wood mice, including Apodemus flavicollis, A. sylvaticus, A. uralensis (=A. microps), A. fulvipectus (=A. falzfeini), A. ponticus, A. hyrcanicus, A. mystacinus, A. agrarius, A. peninsulae, and A. speciosus. Peculiarities of NOR location in karyotypes can be used in interspecific diagnostics of wood mice. Intraspecific polymorphism of A. sylvaticus, A. agrarius, and A. peninsulae in terms of the number of NORs and their localization in chromosomes can serve as evidence for karyological differentiation in certain populations of these species. The minimum number of active NORs in micemore » of the genus Apodemus is two to four. Two A. flavicollis wood mice with karyotypes containing one small acrocentric B-chromosome (2n = 49) were identified among animals captured in Estonia. In A. peninsulae, B-chromosomes were found among animals captured in the following regions: the vicinity of Kyzyl (one mouse with 17 microchromosomes, 2n = 65); the vicinity of Birakan (two mice with one metacentric chromosome each, 2n = 49); and in the Ussuri Nature Reserve (one mouse with five B-chromosomes, including three metacentric and two dotlike chromosomes; 2n = 53). In the latter animal, the presence of NORs on two metacentric B-chromosomes was revealed; this is the first case of identification of active NORs on extra chromosomes of mammals. 29 refs., 4 figs., 1 tab.« less

  2. Production and characterization of activated carbon from wood wastes

    NASA Astrophysics Data System (ADS)

    Ramirez, A. P.; Giraldo, S.; Ulloa, M.; Flórez, E.; Y Acelas, N.

    2017-12-01

    Cedarwood (Cedrela Angustifolia) and teak (Tectona Grandis) woods are typically used for furniture manufacture because they have high durability, are light and easy to work. During these manufacturing process, large amount of these wastes is generated causing disposal environmental problems. In this paper, the residual wastes (sawdust) of Cedar (C) and Teak (T) are transformed into an activated material. The chemical composition of both biomass (C and T) was determinate by TGA (Thermogravimetric Analysis). Activated materials were characterized in surface area following the BET (Brunauer, Emmett and Teller) method, morphology using SEM (Scanning Electron Microscopy) and to know their functional groups a FTIR (Fourier Transform Infrared Spectroscopy) analysis was done. Their adsorption capacity was evaluated by removal of Methylene Blue (MB) and Congo Red (CR) from aqueous solutions.

  3. Surfactant-free carnauba wax dispersion and its use for layer-by-layer assembled protective surface coatings on wood

    NASA Astrophysics Data System (ADS)

    Lozhechnikova, Alina; Bellanger, Hervé; Michen, Benjamin; Burgert, Ingo; Österberg, Monika

    2017-02-01

    Protection from liquid water and UV radiation are equally important, and a sophisticated approach is needed when developing surface coatings that preserve the natural and well-appreciated aesthetic appearance of wood. In order to prevent degradation and prolong the service life of timber, a protective coating was assembled using carnauba wax particles and zinc oxide nanoparticles via layer-by-layer deposition in water. For this purpose, a facile sonication route was developed to produce aqueous wax dispersion without any surfactants or stabilizers. The suspension was stable above pH 4 due to the electrostatic repulsion between the negatively charged wax particles. The particle size could be controlled by the initial wax concentration with average particle sizes ranging from 260 to 360 nm for 1 and 10 g/L, respectively. The deposition of wax particles onto the surface of spruce wood introduced additional roughness to the wood surface at micron level, while zinc oxide provided nano roughness and UV-absorbing properties. In addition to making wood superhydrophobic, this novel multilayer coating enhanced the natural moisture buffering capability of spruce. Moreover, wood surfaces prepared in this fashion showed a significant reduction in color change after exposure to UV light. A degradation of the wax through photocatalytic activity of the ZnO particles was measured by FTIR, indicating that further studies are required to achieve long-term stability. Nevertheless, the developed coating showed a unique combination of superhydrophobicity and excellent moisture buffering ability and some UV protection, all achieved using an environmentally friendly coating process, which is beneficial to retain the natural appearance of wood and improve indoor air quality and comfort.

  4. Increasing the Presence of Underrepresented Minorities in the Geosciences: The Woods Hole Partnership Education Program Model and Outcomes

    NASA Astrophysics Data System (ADS)

    George, A.; Gutierrez, B.; Jearld, A.; Liles, G.; Scott, O.; Harden, B.

    2017-12-01

    Launched in 2009, the Partnership Education Program (PEP) is supported by six scientific institutions in Woods Hole, Massachusetts through the Woods Hole Diversity Initiative. PEP, which was shaped by experience with other diversity programs as well as input from scientists in Woods Hole, is designed to promote a diverse scientific community by recruiting talent from minority groups that are under-represented in marine and environmental sciences. Focused on college juniors and seniors with course work in marine and/or environmental sciences, PEP is comprised of a four-week course, "Ocean and Environmental Sciences: Global Climate Change," and a six to eight week individual research project under the guidance of a research mentor. Investigators from the six science institutions serve as course faculty and research mentors. Course credit is through PEP's academic partner, the University of Maryland Eastern Shore. PEP students also participate in seminars, workshops, field trips, at-sea experiences, career development activities, and attend lectures at participating science institutions throughout the summer. Students present their research results at the end of the summer with a 15-minute public presentation. A number of PEP participants then presented their work at professional and scientific meetings, such as AGU, using the program as a gateway to graduate education and career opportunities in the marine and environmental sciences. From 2009 through 2017, 138 students from 86 colleges and universities, including many that previously had sent few or no students or faculty to Woods Hole, have participated in the program. Participating organizations are: Northeast Fisheries Science Center (NOAA Fisheries), Marine Biological Laboratory (MBL), Sea Education Association (SEA), U.S. Geological Survey (USGS), Woods Hole Oceanographic Institution (WHOI), Woods Hole Research Center (WHRC), and University of Maryland Eastern Shore (UMES) - academic partner.

  5. Polyphenols in strawberry (Fragaria × ananassa) leaves induced by plant activators.

    PubMed

    Kårlund, Anna; Salminen, Juha-Pekka; Koskinen, Piia; Ahern, Jeffrey R; Karonen, Maarit; Tiilikkala, Kari; Karjalainen, Reijo O

    2014-05-21

    Strawberry leaves contain high amounts of diverse phenolic compounds potentially possessing defensive activities against microbial pathogens and beneficial properties for human health. In this work, young strawberry plants were treated with two plant activators, S-methylbenzo-1,2,3-thaidiazole-7-carbothiate (BTH) and birch wood distillate. Phenolic compounds from activator-treated and control leaves were subjected to quantitative analyses by HPLC-DAD, HPLC-ESI-MS, and microQTOF ESI-MS. Thirty-two different phenolic compounds were detected and characterized, and 21 different ellagitannins constituted the largest group of compounds in the strawberry leaves (37.88-45.82 mg/g dry weight, 47.0-54.3% of total phenolics). Treatment with BTH resulted in higher levels of individual ellagitannins, whereas treatment with birch wood distillate strongly increased the levels of chlorogenic acid in strawberry leaves compared with the control. The results suggest that different plant activators may be useful tools for the activation of different branches in the phenylpropanoid biosynthesis in strawberry.

  6. Massive Organic Matter Fall Processing and Organic Enrichment in Deep Sea Sediments: the Role of Fall Type

    NASA Astrophysics Data System (ADS)

    Hannides, A. K.; Smith, C. R.; Baco-Taylor, A. R.

    2005-12-01

    Resource-limited deep-sea sedimentary settings are occasionally punctuated with massive organic matter (MOM) falls, such as fish and marine mammal carcasses, macrophytes and wood. In the case of whale falls, previous studies have shown that sharp gradients in microbial activity exist within a few meters of these falls. These sites are characterized by intense sulfate reduction and sulfide generation, which are commonly attributed to sedimentary organic enrichment from MOM, and in part support extensive chemosynthetic communities that rely on endosymbiotic oxidation of this sulfide for energy. Enrichment is brought about by the fragmentation and dissemination activities of deep-sea megafauna: scavengers in the case of carcasses and macrophytes, and wood borers in the case of wood.Differences in MOM fall composition and structure and the subsequent megafaunal processing raise questions concerning the patterns of organic enrichment around these falls and how these patterns vary with fall type. We present an extensive data set of surface sediment organic carbon and nitrogen content at whale, kelp and wood falls of various ages in the California Borderland Basins region. Evidence for organic enrichment around whale falls is lacking, corroborating previous findings. However, distinctly low C:N ratios in surface sediments adjacent to whale falls suggest more complex processing of MOM in this zone. This pattern persists regardless of whale fall age. On the contrary, evidence for organic enrichment around kelp and wood falls abounds. Organic carbon and nitrogen content values adjacent to 3 month-old kelp falls are 25-50 % higher than those 1 m away from the falls and traces of this signal persist for at least 3 more months. In the case of wood falls, 6 month-old falls do not show any significant traces of enrichment, but 3 years after the fall event organic carbon content adjacent to the falls increases by 2-5 times that of background. C:N ratios concomitantly increase to startling levels of 80-100. Comparison of falls of different ages but similar masses reveals that kelp falls result in rapid (<3 months) enrichment peaks, while wood falls only generate significant signals on the order of years. Comparison of organic enrichment patterns with pore water sulfide distributions suggests a role of MOM type or quality in stimulating increases in sedimentary organic matter decomposition rates.

  7. MASSAHAKE whole tree harvesting method for pulp raw-material and fuel -- R&D in 1993--1998

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Asplund, D.A.; Ahonen, M.A.

    1993-12-31

    In Finland biofuels and hydropower are the only indigenous fuels available. Peat, wood and wood derived fuels form about 18% of total primary energy requirement. The largest wood and wood fuel user in Finland is wood processing industry, paper, pulp, sawmills. Due to silvicultural activities the growth of forests has developed an instant need for first thinnings. This need is about 12% of total stem wood growth. With conventional harvesting methods this would produce about 8 mill. m{sup 3} pulp raw material and 2 mill. m{sup 3} wood fuel. By using integrated harvesting methods about 12 mill. m{sup 3} pulpmore » raw material and 8 mill. m{sup 3} (about 1, 3 mill. toe) fuel could be produced. At the moment, there is no economically profitable method for harvesting first thinning trees for industrial use or energy production. Hence, there are a few ongoing research projects aiming at solving the question of integrated harvesting. MASSAHAKE chip purification method has been under R&D since 1987. Research with continuous experimental line (capacity 5--10 loose-m{sup 3}) has been done in 1991 and 1992. The research has concentrated on pine whole tree chip treatment, but preliminary tests with birch whole tree chips has been done. The experiment line will be modified for birth whole tree chips during 1993. Based on the research results more than 60% of the whole tree chips can be separated to pulp raw material with < 1% bark content. This amount is 1.5--2 times more than with present technology. The yield of fuel fraction is 2--4 times higher compared to present methods. Fuel fraction is homogeneous and could be used in most furnaces for energy production. By replacing fossil fuels with wood fuel in energy production it is possible to reduce CO{sub 2}-emissions significantly. This paper presents the wood fuel research areas in Finland and technical potential of MASSAHAKE-method including the plant for building a demonstration plant based on this technology.« less

  8. In situ modification of activated carbons developed from a native invasive wood on removal of trace toxic metals from wastewater.

    PubMed

    de Celis, J; Amadeo, N E; Cukierman, A L

    2009-01-15

    Activated carbons were developed by phosphoric acid activation of sawdust from Prosopis ruscifolia wood, an indigenous invasive species of degraded lands, at moderate conditions (acid/precursor ratio=2, 450 degrees C, 0.5h). For in situ modification of their characteristics, either a self-generated atmosphere or flowing air was used. The activated carbons developed in the self-generated atmosphere showed higher BET surface area (2281m2/g) and total pore volume (1.7cm3/g) than those obtained under flowing air (1638m2/g and 1.3cm3/g). Conversely, the latter possessed a higher total amount of surface acidic/polar oxygen groups (2.2meq/g) than the former (1.5meq/g). To evaluate their metal sorption capability, adsorption isotherms of Cu(II) ion from model solutions were determined and properly described by the Langmuir model. Maximum sorption capacity (Xm) for the air-derived carbons (Xm=0.44mmol/g) almost duplicated the value for those obtained in the self-generated atmosphere (Xm=0.24mmol/g), pointing to a predominant effect of the surface functionalities on metal sequestering behaviour. The air-derived carbons also demonstrated a superior effectiveness in removing Cd(II) ions as determined from additional assays in equilibrium conditions. Accordingly, effective phosphoric acid-activated carbons from Prosopis wood for toxic metals removal from wastewater may be developed by in situ modification of their characteristics operating under flowing air.

  9. Modification of lignin for the production of new compounded materials.

    PubMed

    Hüttermann, A; Mai, C; Kharazipour, A

    2001-05-01

    The cell walls of woody plants are compounded materials made by in situ polymerization of a polyphenolic matrix (lignin) into a web of fibers (cellulose), a process that is catalysed by polyphenoloxidases (laccases) or peroxidases. The first attempt to transform the basic strategy of this natural process for use in human craftsmanship was the ancient lacquer method. The sap of the lacquer tree (Rhus verniciflua) contains large amounts of a phenol (urushiol), a polysaccharide and the enzyme laccase. This oil-in-water emulsion solidifies in the presence of oxygen. The Chinese began using this phenomenon for the production of highly creative artwork more than 6,000 years ago. It was the first example of an isolated enzyme being used as a catalyst to create an artificial plastic compound. In order to apply this process to the production of products on an industrial scale, an inexpensive phenol must be used, which is transferred by an enzyme to active radicals that react with different components to form a compounded material. At present, the following approaches have been studied: (1) In situ polymerization of lignin for the production of particle boards. Adhesive cure is based on the oxidative polymerization of lignin using phenoloxidases (laccase) as radical donors. This lignin-based bio-adhesive can be applied under conventional pressing conditions. The resulting particle boards meet German performance standards. By this process, 80% of the petrochemical binders in the wood-composite industry can be replaced by materials from renewable resources. (2) Enzymatic copolymerization of lignin and alkenes. In the presence of organic hydroperoxides, laccase catalyses the reaction between lignin and olefins. Detailed studies on the reaction between lignin and acrylate monomers showed that chemo-enzymatic copolymerization offers the possibility to produce defined lignin-acrylate copolymers. The system allows control of the molecular weights of the products in a way that has not been possible with chemical catalysts. This is a novel attempt to enzymatically induce grafting of polymeric side chains onto the lignin backbone, and it enables the utilization of lignin as part of new engineering materials. (3) Enzymatic activation of the middle-lamella lignin of wood fibers for the production of wood composites. The incubation of wood fibers with a phenol oxidizing enzyme results in oxidative activation of the lignin crust on the fiber surface. When such fibers are pressed together, boards are obtained which meet the German standards for medium-density fiber boards (MDF). The fibers are bound together in a way that comes close to that by which wood fibers are bound together in naturally grown wood. This process will, for the first time, yield wood composites that are produced solely from naturally grown products without any addition of resins.

  10. Product distribution from pyrolysis of wood and agricultural residues

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Di Blasi, C.; Signorelli, G.; Di Russo, C.

    1999-06-01

    The pyrolysis characteristics of agricultural residues (wheat straw, olive husks, grape residues, and rice husks) and wood chips have been investigated on a bench scale. The experimental system establishes the conditions encountered by a thin (4 {times} 10{sup {minus}2} m diameter) packed bed of biomass particles suddenly exposed in a high-temperature environment, simulated by a radiant furnace. Product yields (gases, liquids, and char) and gas composition, measured for surface bed temperatures in the range 650--1000 K, reproduce trends already observed for wood. However, differences are quantitatively large. Pyrolysis of agricultural residues is always associated with much higher solid yields (upmore » to a factor of 2) and lower liquid yields. Differences are lower for the total gas, and approximate relationships exist among the ratios of the main gas species yields, indicating comparable activation energies for the corresponding apparent kinetics of formation. However, while the ratios are about the same for wood chips, rice husks, and straw, much lower values are shown by olive and grape residues. Large differences have also been found in the average values of the specific devolatilization rates. The fastest (up to factors of about 1.5 with respect to wood) have been observed for wheat straw and the slowest (up to factors of 2) for grape residues.« less

  11. Synchrotron-based X-ray fluorescence microscopy enables multiscale spatial visualization of ions involved in fungal lignocellulose deconstruction

    NASA Astrophysics Data System (ADS)

    Kirker, Grant; Zelinka, Sam; Gleber, Sophie-Charlotte; Vine, David; Finney, Lydia; Chen, Si; Hong, Young Pyo; Uyarte, Omar; Vogt, Stefan; Jellison, Jody; Goodell, Barry; Jakes, Joseph E.

    2017-01-01

    The role of ions in the fungal decay process of lignocellulose biomaterials, and more broadly fungal metabolism, has implications for diverse research disciplines ranging from plant pathology and forest ecology, to carbon sequestration. Despite the importance of ions in fungal decay mechanisms, the spatial distribution and quantification of ions in lignocellulosic cell walls and fungal hyphae during decay is not known. Here we employ synchrotron-based X-ray fluorescence microscopy (XFM) to map and quantify physiologically relevant ions, such as K, Ca, Mn, Fe, and Zn, in wood being decayed by the model brown rot fungus Serpula lacrymans. Two-dimensional XFM maps were obtained to study the ion spatial distributions from mm to submicron length scales in wood, fungal hyphae with the dried extracellular matrix (ECM) from the fungus, and Ca oxalate crystals. Three-dimensional ion volume reconstructions were also acquired of wood cell walls and hyphae with ECM. Results show that the fungus actively transports some ions, such as Fe, into the wood and controls the distribution of ions at both the bulk wood and cell wall length scales. These measurements provide new insights into the movement of ions during decay and illustrate how synchrotron-based XFM is uniquely suited study these ions.

  12. The Archer’s Tale: An Examination of English Archers during the Hundred Years War and Their Impact on Warfare and Society

    DTIC Science & Technology

    2015-06-12

    Grey is also a valuable resource. Grey is a fourteenth century English knight who participated in many of the campaigns against the Scots , and...similar proportions dating from 2400 B.C.E. to 900 C.E. have been found throughout northern Europe. Given how easily wood is destroyed by age, the...their incomes through a variety of activities including wage labor, brewing, small crafts, cutting and collecting wood and peat, and hunting and

  13. Wood Bark Smoke Induces Lung and Pleural Plasminogen Activator Inhibitor 1 and Stabilizes Its mRNA in Porcine Lung Cells

    DTIC Science & Technology

    2011-08-01

    admitted to US burn centers, and greatly increases postburn morbidity and mortality (1). The pathogenesis of smoke inhalationYinduced acute lung...have been successfully used to ameliorate lung dysfunction in SIALI in animal models (3Y5). Disordered fibrin turnover in the lung in patients with...of the pathogenesis of SIALI. In vivo and in vitro approaches were applied to address this gap. We used a porcine model of wood bark smoke (WBS)Y

  14. Synthesis and characterization of a novel mucoadhesive derivative of xyloglucan.

    PubMed

    Madgulkar, Ashwini R; Bhalekar, Mangesh R; Asgaonkar, Kalyani D; Dikpati, Amrita A

    2016-01-01

    A novel polymer in the form of a thiolated derivative of natural tamarind seed polysaccharide or xyloglucan was synthesized and its chacteristics as a mucoadhesive polymer were studied as a part of the study undertaken herein. The synthetic route followed involves a two-step reaction mechanism of firstly oxidizing xyloglucan and then further conjugating it with l-cysteine to form thiolated xyloglucan or thiomer via imine linkage. The thiomer thus formed was characterized using various analytical techniques as differential scanning calorimetry (DSC), X-ray diffraction analysis (XRD), and nuclear magnetic resonance (NMR). Ellman's method was used to determine the numbers of thiol groups/g of thiolated xyloglucan. Zeta potential measurements were carried out for thiolated xyloglucan. Viscosities of the formulated xyloglucan and thiolated xyloglucan gels were comparatively evaluated along with the evaluation of mucoadhesive properties of the gels using ex vivo bioadhesion study employing freshly excised sheep intestinal mucosa. Copyright © 2015 Elsevier Ltd. All rights reserved.

  15. A Comprehensive Review on Rasam: A South Indian Traditional Functional Food.

    PubMed

    Devarajan, Agilandeswari; Mohanmarugaraja, M K

    2017-01-01

    The view that food can have an expanded role that goes well beyond providing a source of nutrients truly applies to traditional functional foods. The systematic consumption of such traditional functional food provides an excellent preventive measure to ward off many diseases. Rasam , a soup of spices, is a traditional South Indian food. It is traditionally prepared using tamarind juice as a base, with the addition of Indian sesame oil, turmeric, tomato, chili pepper, pepper, garlic, cumin, curry leaves, mustard, coriander, asafoetida, sea salt, and water. Rasam is a classic example of traditional functional food with all its ingredients medicinally claimed for various ailments. The preclinical and clinical studies on rasam and its ingredients support their traditional claim. This review is an attempt to compile the literatures on rasam , its ingredients, and to highlight its medicinal potential that has been underestimated.

  16. A Comprehensive Review on Rasam: A South Indian Traditional Functional Food

    PubMed Central

    Devarajan, Agilandeswari; Mohanmarugaraja, M. K.

    2017-01-01

    The view that food can have an expanded role that goes well beyond providing a source of nutrients truly applies to traditional functional foods. The systematic consumption of such traditional functional food provides an excellent preventive measure to ward off many diseases. Rasam, a soup of spices, is a traditional South Indian food. It is traditionally prepared using tamarind juice as a base, with the addition of Indian sesame oil, turmeric, tomato, chili pepper, pepper, garlic, cumin, curry leaves, mustard, coriander, asafoetida, sea salt, and water. Rasam is a classic example of traditional functional food with all its ingredients medicinally claimed for various ailments. The preclinical and clinical studies on rasam and its ingredients support their traditional claim. This review is an attempt to compile the literatures on rasam, its ingredients, and to highlight its medicinal potential that has been underestimated. PMID:28989243

  17. Scientific Insights in the Preparation and Characterisation of a Lead-based Naga Bhasma.

    PubMed

    Nagarajan, S; Krishnaswamy, S; Pemiah, Brindha; Rajan, K S; Krishnan, Umamaheswari; Sethuraman, S

    2014-01-01

    Naga bhasma is one of the herbo-metallic preparations used in Ayurveda, a traditional Indian System of Medicine. The preparation of Naga bhasma involves thermal treatment of 'Naga' (metallic lead) in a series of quenching liquids, followed by reaction with realgar and herbal constituents, before calcination to prepare a fine product. We have analysed the intermediates obtained during different stages of preparation to understand the relevance and importance of different steps involved in the preparation. Our results show that 'Sodhana' (purification process) removes heavy metals other than lead, apart from making it soft and amenable for trituration. The use of powders of tamarind bark and peepal bark maintains the oxidation state of lead in Jarita Naga (lead oxide) as Pb(2+). The repeated calcination steps result in the formation of nano-crystalline lead sulphide, the main chemical species present in Naga bhasma.

  18. Scientific Insights in the Preparation and Characterisation of a Lead-based Naga Bhasma

    PubMed Central

    Nagarajan, S.; Krishnaswamy, S.; Pemiah, Brindha; Rajan, K. S.; Krishnan, Umamaheswari; Sethuraman, S.

    2014-01-01

    Naga bhasma is one of the herbo-metallic preparations used in Ayurveda, a traditional Indian System of Medicine. The preparation of Naga bhasma involves thermal treatment of ‘Naga’ (metallic lead) in a series of quenching liquids, followed by reaction with realgar and herbal constituents, before calcination to prepare a fine product. We have analysed the intermediates obtained during different stages of preparation to understand the relevance and importance of different steps involved in the preparation. Our results show that ‘Sodhana’ (purification process) removes heavy metals other than lead, apart from making it soft and amenable for trituration. The use of powders of tamarind bark and peepal bark maintains the oxidation state of lead in Jarita Naga (lead oxide) as Pb2+. The repeated calcination steps result in the formation of nano-crystalline lead sulphide, the main chemical species present in Naga bhasma. PMID:24799737

  19. Drivers of aboveground wood production in a lowland tropical forest of West Africa: teasing apart the roles of tree density, tree diversity, soil phosphorus, and historical logging.

    PubMed

    Jucker, Tommaso; Sanchez, Aida Cuni; Lindsell, Jeremy A; Allen, Harriet D; Amable, Gabriel S; Coomes, David A

    2016-06-01

    Tropical forests currently play a key role in regulating the terrestrial carbon cycle and abating climate change by storing carbon in wood. However, there remains considerable uncertainty as to whether tropical forests will continue to act as carbon sinks in the face of increased pressure from expanding human activities. Consequently, understanding what drives productivity in tropical forests is critical. We used permanent forest plot data from the Gola Rainforest National Park (Sierra Leone) - one of the largest tracts of intact tropical moist forest in West Africa - to explore how (1) stand basal area and tree diversity, (2) past disturbance associated with past logging, and (3) underlying soil nutrient gradients interact to determine rates of aboveground wood production (AWP). We started by statistically modeling the diameter growth of individual trees and used these models to estimate AWP for 142 permanent forest plots. We then used structural equation modeling to explore the direct and indirect pathways which shape rates of AWP. Across the plot network, stand basal area emerged as the strongest determinant of AWP, with densely packed stands exhibiting the fastest rates of AWP. In addition to stand packing density, both tree diversity and soil phosphorus content were also positively related to productivity. By contrast, historical logging activities negatively impacted AWP through the removal of large trees, which contributed disproportionately to productivity. Understanding what determines variation in wood production across tropical forest landscapes requires accounting for multiple interacting drivers - with stand structure, tree diversity, and soil nutrients all playing a key role. Importantly, our results also indicate that logging activities can have a long-lasting impact on a forest's ability to sequester and store carbon, emphasizing the importance of safeguarding old-growth tropical forests.

  20. Conditions Affecting Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) Basidiome Quality, Morphogenesis, and Biodegradation of Wood By-products in Argentina.

    PubMed

    Kuhar, Francisco; Postemsky, Pablo Daniel; Bianchinotti, Maria Virginia

    2018-01-01

    Solid-state fermentation (SSF) with the medicinal higher Basidiomycete Ganoderma lucidum was studied as a strategy to use pine (Pinus radiata D. Don) and poplar (Populus nigra L.) wood chips and sawdust. Fruiting bodies were produced and the value of the biotransformed substrate was assessed. The highest mushroom yield (63 g dry weight per kilogram of dry substrate) was obtained with poplar sawdust and wood chips. Immersion of the bioreactors was a simple watering method that obtained suitable yields. Two morphological types were induced using 2 different incandescent light intensities. High light irradiation induced the highest valued mushroom morphology (as a whole product). Time course study of substrate biodegradation and mycelial growth dynamics indicated that the trophophase lasted 20 days and presented laccase activity of 0.01-0.03 units · g-1. The activity at idiophase was 10 times higher. Aqueous and alkali extracts, as well as carbohydrase enzyme profile activity, revealed differences in the properties of the residual substrate; some related to the substrate source are considered to be of concern for further use of this pretreated biomass. In view of the results obtained, we propose use of SSF of pine and poplar with G. lucidum to profitably recycle softwood by-products from the timber industry.

  1. Significance of wood extractives for wood bonding.

    PubMed

    Roffael, Edmone

    2016-02-01

    Wood contains primary extractives, which are present in all woods, and secondary extractives, which are confined in certain wood species. Extractives in wood play a major role in wood-bonding processes, as they can contribute to or determine the bonding relevant properties of wood such as acidity and wettability. Therefore, extractives play an immanent role in bonding of wood chips and wood fibres with common synthetic adhesives such as urea-formaldehyde-resins (UF-resins) and phenol-formaldehyde-resins (PF-resins). Extractives of high acidity accelerate the curing of acid curing UF-resins and decelerate bonding with alkaline hardening PF-resins. Water-soluble extractives like free sugars are detrimental for bonding of wood with cement. Polyphenolic extractives (tannins) can be used as a binder in the wood-based industry. Additionally, extractives in wood can react with formaldehyde and reduce the formaldehyde emission of wood-based panels. Moreover, some wood extractives are volatile organic compounds (VOC) and insofar also relevant to the emission of VOC from wood and wood-based panels.

  2. Analysts guide to FEEMA for financial analysis of ecosystem management activities.

    Treesearch

    Roger D. Fight; John T. Chmelik

    1998-01-01

    This report describes strategies for using the Financial Evaluation of Ecosystem Management Activities (FEEMA) software. This program was developed as a tool for assessing the financial viability of management activities for removing small trees for manufacture into wood products. Combinations of tree stands, management activities, and contractual requirements can be...

  3. Immunomodulatory Activity of Xanthones from Calophyllum teysmannii var. inuphylloide.

    PubMed

    Gonzalez, M J; Nascimento, M S; Cidade, H M; Pinto, M M; Kijjoa, A; Anantachoke, C; Silva, A M; Herz, W

    1999-05-01

    Nine xanthones, including 3-(4-hydroxy-3-metnylbutyl)-4,8-dihydroxyxanthone, were isolated from the wood of a Thai collection of CALOPHYLLUM TEYSMANNII Miq. var. INUPHYLLOIDE (King) P. Stephen. Immunomodulatory activities of eight of these have been investigated.

  4. Wood : adhesives

    Treesearch

    A.H. Conner

    2001-01-01

    This chapter on wood adhesives includes: 1) Classification of wood adhesives 2) Thermosetting wood adhesives 3) Thermoplastic adhesives, 4) Wood adhesives based on natural sources 5) Nonconventional bonding of wood 6) Wood bonding.

  5. In the Style of Henry Moore

    ERIC Educational Resources Information Center

    Hinshaw, Craig

    2010-01-01

    In this article, the author describes an art project inspired by Henry Moore's sculptures. This project consists of two activities. In the first activity, students select, sand and stain a wood block that would become a base for their plaster sculpture. This activity would keep the students independently engaged (classroom management) while the…

  6. TAILORING ACTIVATED CARBONS FOR ENHANCED REMOVAL OF NATURAL ORGANIC MATTER FROM NATURAL WATERS. (R828157)

    EPA Science Inventory

    Several pathways have been employed to systematically modify two granular activated carbons (GACs), F400 (coal-based) and Macro (wood-based), for examining adsorption of dissolved natural organic matter (DOM) from natural waters. A total of 24 activated carbons with different ...

  7. Agricultural Education Science Activity--Nos. AEM 1-4.

    ERIC Educational Resources Information Center

    Ohio State Univ., Columbus. Agricultural Curriculum Materials Service.

    This packet contains four science learning activities that can be used in agricultural education courses. The activities cover these topics: (1) determining the effect of air pressure on fluid flow; (2) how lubrication and oil viscosity affect friction; (3) determining relative strengths of wood fasteners; and (4) determining the effects of…

  8. Third Quarter Hanford Seismic Report for Fiscal Year 2009

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Rohay, Alan C.; Sweeney, Mark D.; Hartshorn, Donald C.

    2009-09-30

    The Hanford Seismic Assessment Program (HSAP) provides an uninterrupted collection of high-quality raw and processed seismic data from the Hanford Seismic Network for the U.S. Department of Energy and its contractors. The HSAP is responsible for locating and identifying sources of seismic activity and monitoring changes in the historical pattern of seismic activity at the Hanford Site. The data are compiled, archived, and published for use by the Hanford Site for waste management, natural phenomena hazards assessments, and engineering design and construction. In addition, the HSAP works with the Hanford Site Emergency Services Organization to provide assistance in the eventmore » of a significant earthquake on the Hanford Site. The Hanford Seismic Network and the Eastern Washington Regional Network consist of 44 individual sensor sites and 15 radio relay sites maintained by the Hanford Seismic Assessment Team. The Hanford Seismic Network recorded 771 local earthquakes during the third quarter of FY 2009. Nearly all of these earthquakes were detected in the vicinity of Wooded Island, located about eight miles north of Richland just west of the Columbia River. The Wooded Island events recorded this quarter is a continuation of the swarm events observed during the January – March 2009 time period and reported in the previous quarterly report (Rohay et al, 2009). The frequency of Wooded Island events has subsided with 16 events recorded during June 2009. Most of the events were considered minor (magnitude (Mc) less than 1.0) with 25 events in the 2.0-3.0 range. The estimated depths of the Wooded Island events are shallow (averaging less than 1.0 km deep) with a maximum depth estimated at 2.2 km. This places the Wooded Island events within the Columbia River Basalt Group (CRBG). The low magnitude of the Wooded Island events has made them undetectable to all but local area residents. However, some Hanford employees working within a few miles of the area of highest activity and individuals living in homes directly across the Columbia River from the swarm center have reported feeling many of the larger magnitude events. The Hanford Strong Motion Accelerometer (SMA) network was triggered numerous times by the Wooded Island swarm events. The maximum acceleration value recorded by the SMA network was approximately 3 times lower than the reportable action level for Hanford facilities (2% g) and no action was required. The swarming is likely due to pressure that has built up, cracking the brittle basalt layers within the Columbia River Basalt Formation (CRBG). Similar earthquake “swarms” have been recorded near this same location in 1970, 1975 and 1988. Prior to the 1970s, swarming may have occurred, but equipment was not in place to record those events. Quakes of this limited magnitude do not pose a risk to Hanford cleanup efforts or waste storage facilities. Since swarms of the past did not intensify in magnitude, seismologists do not expect that these events will increase in intensity. However, Pacific Northwest National Laboratory (PNNL) will continue to monitor the activity.« less

  9. Nest sanitation through defecation: antifungal properties of wood cockroach feces.

    PubMed

    Rosengaus, Rebeca B; Mead, Kerry; Du Comb, William S; Benson, Ryan W; Godoy, Veronica G

    2013-11-01

    The wood cockroach Cryptocercus punctulatus nests as family units inside decayed wood, a substrate known for its high microbial load. We tested the hypothesis that defecation within their nests, a common occurrence in this species, reduces the probability of fungal development. Conidia of the entomopathogenic fungus, Metarhizium anisopliae, were incubated with crushed feces and subsequently plated on potato dextrose agar. Relative to controls, the viability of fungal conidia was significantly reduced following incubation with feces and was negatively correlated with incubation time. Although the cockroach's hindgut contained abundant β-1,3-glucanase activity, its feces had no detectable enzymatic function. Hence, these enzymes are unlikely the source of the fungistasis. Instead, the antifungal compound(s) of the feces involved heat-sensitive factor(s) of potential microbial origin. When feces were boiled or when they were subjected to ultraviolet radiation and subsequently incubated with conidia, viability was "rescued" and germination rates were similar to those of controls. Filtration experiments indicate that the fungistatic activity of feces results from chemical interference. Because Cryptocercidae cockroaches have been considered appropriate models to make inferences about the factors fostering the evolution of termite sociality, we suggest that nesting in microbe-rich environments likely selected for the coupling of intranest defecation and feces fungistasis in the common ancestor of wood cockroaches and termites. This might in turn have served as a preadaptation that prevented mycosis as these phylogenetically related taxa diverged and evolved respectively into subsocial and eusocial organizations.

  10. Trichoderma species occurring on wood with decay symptoms in mountain forests in Central Europe: genetic and enzymatic characterization.

    PubMed

    Błaszczyk, Lidia; Strakowska, Judyta; Chełkowski, Jerzy; Gąbka-Buszek, Agnieszka; Kaczmarek, Joanna

    2016-08-01

    The aim of this study was to explore the species diversity of Trichoderma obtained from samples of wood collected in the forests of the Gorce Mountains (location A), Karkonosze Mountains (location B) and Tatra Mountains (location C) in Central Europe and to examine the cellulolytic and xylanolytic activity of these species as an expression of their probable role in wood decay processes. The present study has led to the identification of the following species and species complex: Trichoderma atroviride P. Karst., Trichoderma citrinoviride Bissett, Trichoderma cremeum P. Chaverri & Samuels, Trichoderma gamsii Samuels & Druzhin., Trichoderma harzianum complex, Trichoderma koningii Oudem., Trichoderma koningiopsis Samuels, C. Suárez & H.C. Evans, Trichoderma longibrachiatum Rifai, Trichoderma longipile Bissett, Trichoderma sp. (Hypocrea parapilulifera B.S. Lu, Druzhin. & Samuels), Trichoderma viride Schumach. and Trichoderma viridescens complex. Among them, T. viride was observed as the most abundant species (53 % of all isolates) in all the investigated locations. The Shannon's biodiversity index (H), evenness (E), and the Simpson's biodiversity index (D) calculations for each location showed that the highest species diversity and evenness were recorded for location A-Gorce Mountains (H' = 1.71, E = 0.82, D = 0.79). The preliminary screening of 119 Trichoderma strains for cellulolytic and xylanolytic activity showed the real potential of all Trichoderma species originating from wood with decay symptoms to produce cellulases and xylanases-the key enzymes in plant cell wall degradation.

  11. Nest sanitation through defecation: antifungal properties of wood cockroach feces

    NASA Astrophysics Data System (ADS)

    Rosengaus, Rebeca B.; Mead, Kerry; Du Comb, William S.; Benson, Ryan W.; Godoy, Veronica G.

    2013-11-01

    The wood cockroach Cryptocercus punctulatus nests as family units inside decayed wood, a substrate known for its high microbial load. We tested the hypothesis that defecation within their nests, a common occurrence in this species, reduces the probability of fungal development. Conidia of the entomopathogenic fungus, Metarhizium anisopliae, were incubated with crushed feces and subsequently plated on potato dextrose agar. Relative to controls, the viability of fungal conidia was significantly reduced following incubation with feces and was negatively correlated with incubation time. Although the cockroach's hindgut contained abundant β-1,3-glucanase activity, its feces had no detectable enzymatic function. Hence, these enzymes are unlikely the source of the fungistasis. Instead, the antifungal compound(s) of the feces involved heat-sensitive factor(s) of potential microbial origin. When feces were boiled or when they were subjected to ultraviolet radiation and subsequently incubated with conidia, viability was "rescued" and germination rates were similar to those of controls. Filtration experiments indicate that the fungistatic activity of feces results from chemical interference. Because Cryptocercidae cockroaches have been considered appropriate models to make inferences about the factors fostering the evolution of termite sociality, we suggest that nesting in microbe-rich environments likely selected for the coupling of intranest defecation and feces fungistasis in the common ancestor of wood cockroaches and termites. This might in turn have served as a preadaptation that prevented mycosis as these phylogenetically related taxa diverged and evolved respectively into subsocial and eusocial organizations.

  12. Variation in wood nutrients along a tropical soil fertility gradient.

    PubMed

    Heineman, Katherine D; Turner, Benjamin L; Dalling, James W

    2016-07-01

    Wood contains the majority of the nutrients in tropical trees, yet controls over wood nutrient concentrations and their function are poorly understood. We measured wood nutrient concentrations in 106 tree species in 10 forest plots spanning a regional fertility gradient in Panama. For a subset of species, we quantified foliar nutrients and wood density to test whether wood nutrients scale with foliar nutrients at the species level, or wood nutrient storage increases with wood density as predicted by the wood economics spectrum. Wood nutrient concentrations varied enormously among species from fourfold in nitrogen (N) to > 30-fold in calcium (Ca), potassium (K), magnesium (Mg) and phosphorus (P). Community-weighted mean wood nutrient concentrations correlated positively with soil Ca, K, Mg and P concentrations. Wood nutrients scaled positively with leaf nutrients, supporting the hypothesis that nutrient allocation is conserved across plant organs. Wood P was most sensitive to variation in soil nutrient availability, and significant radial declines in wood P indicated that tropical trees retranslocate P as sapwood transitions to heartwood. Wood P decreased with increasing wood density, suggesting that low wood P and dense wood are traits associated with tree species persistence on low fertility soils. Substantial variation among species and communities in wood nutrient concentrations suggests that allocation of nutrients to wood, especially P, influences species distributions and nutrient dynamics in tropical forests. © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

  13. Effect of cement/wood ratios and wood storage conditions on hydration temperature, hydration time, and compressive strength of wood-cement mixtures

    Treesearch

    Andy W.C. Lee; Zhongli Hong; Douglas R. Phillips; Chung-Yun Hse

    1987-01-01

    This study investigated the effect of cement/wood ratios and wood storage conditions on hydration temperature, hydration time, and compressive strength of wood-cement mixtures made from six wood species: southern pine, white oak, southern red oak, yellow-poplar, sweetgum, and hickory. Cement/wood ratios varied from 13/1 to 4/1. Wood storage conditions consisted of air-...

  14. Wood handbook : wood as an engineering material.

    Treesearch

    Forest Products Laboratory

    1999-01-01

    Summarizes information on wood as an engineering material. Presents properties of wood and wood-based products of particular concern to the architect and engineer. Includes discussion of designing with wood and wood-based products along with some pertinent uses.

  15. Wood handbook : wood as an engineering material

    Treesearch

    Robert J. Ross; Forest Products Laboratory USDA Forest Service.

    2010-01-01

    Summarizes information on wood as an engineering material. Presents properties of wood and wood-based products of particular concern to the architect and engineer. Includes discussion of designing with wood and wood-based products along with some pertinent uses.

  16. Enzyme Activities of Two Recombinant Heme-Containing Peroxidases, TvDyP1 and TvVP2, Identified from the Secretome of Trametes versicolor.

    PubMed

    Amara, Sawsan; Perrot, Thomas; Navarro, David; Deroy, Aurélie; Benkhelfallah, Amine; Chalak, Amani; Daou, Marianne; Chevret, Didier; Faulds, Craig B; Berrin, Jean-Guy; Morel-Rouhier, Mélanie; Gelhaye, Eric; Record, Eric

    2018-04-15

    Trametes versicolor is a wood-inhabiting agaricomycete known for its ability to cause strong white-rot decay on hardwood and for its high tolerance of phenolic compounds. The goal of the present work was to gain insights into the molecular biology and biochemistry of the heme-including class II and dye-decolorizing peroxidases secreted by this fungus. Proteomic analysis of the secretome of T. versicolor BRFM 1218 grown on oak wood revealed a set of 200 secreted proteins, among which were the dye-decolorizing peroxidase Tv DyP1 and the versatile peroxidase Tv VP2. Both peroxidases were heterologously produced in Escherichia coli , biochemically characterized, and tested for the ability to oxidize complex substrates. Both peroxidases were found to be active against several substrates under acidic conditions, and Tv DyP1 was very stable over a relatively large pH range of 2.0 to 6.0, while Tv VP2 was more stable at pH 5.0 to 6.0 only. The thermostability of both enzymes was also tested, and Tv DyP1 was globally found to be more stable than Tv VP2. After 180 min of incubation at temperatures ranging from 30 to 50°C, the activity of Tv VP2 drastically decreased, with 10 to 30% of the initial activity retained. Under the same conditions, Tv DyP1 retained 20 to 80% of its enzyme activity. The two proteins were catalytically characterized, and Tv VP2 was shown to accept a wider range of reducing substrates than Tv DyP1. Furthermore, both enzymes were found to be active against two flavonoids, quercetin and catechin, found in oak wood, with Tv VP2 displaying more rapid oxidation of the two compounds. They were tested for the ability to decolorize five industrial dyes, and Tv VP2 presented a greater ability to oxidize and decolorize the dye substrates than Tv DyP1. IMPORTANCE Trametes versicolor is a wood-inhabiting agaricomycete known for its ability to cause strong white-rot decay on hardwood and for its high tolerance of phenolic compounds. Among white-rot fungi, the basidiomycete T. versicolor has been extensively studied for its ability to degrade wood, specifically lignin, thanks to an extracellular oxidative enzymatic system. The corresponding oxidative system was previously studied in several works for classical lignin and manganese peroxidases, and in this study, two new components of the oxidative system of T. versicolor , one dye-decolorizing peroxidase and one versatile peroxidase, were biochemically characterized in depth and compared to other fungal peroxidases. Copyright © 2018 American Society for Microbiology.

  17. Early Results of Three-Year Monitoring of Red Wood Ants’ Behavioral Changes and Their Possible Correlation with Earthquake Events

    PubMed Central

    Berberich, Gabriele; Berberich, Martin; Grumpe, Arne; Wöhler, Christian; Schreiber, Ulrich

    2013-01-01

    Simple Summary For three years (2009–2012), two red wood ant mounds (Formica rufa-group), located at the seismically active Neuwied Basin (Eifel, Germany), have been monitored 24/7 by high-resolution cameras. Early results show that ants have a well-identifiable standard daily routine. Correlation with local seismic events suggests changes in the ants’ behavior hours before the earthquake: the nocturnal rest phase and daily activity are suppressed, and standard daily routine does not resume until the next day. At present, an automated image evaluation routine is being applied to the video streams. Based on this automated approach, a statistical analysis of the ant behavior will be carried out. Abstract Short-term earthquake predictions with an advance warning of several hours or days are currently not possible due to both incomplete understanding of the complex tectonic processes and inadequate observations. Abnormal animal behaviors before earthquakes have been reported previously, but create problems in monitoring and reliability. The situation is different with red wood ants (RWA; Formica rufa-group (Hymenoptera: Formicidae)). They have stationary mounds on tectonically active, gas-bearing fault systems. These faults may be potential earthquake areas. For three years (2009–2012), two red wood ant mounds (Formica rufa-group), located at the seismically active Neuwied Basin (Eifel, Germany), have been monitored 24/7 by high-resolution cameras with both a color and an infrared sensor. Early results show that ants have a well-identifiable standard daily routine. Correlation with local seismic events suggests changes in the ants’ behavior hours before the earthquake: the nocturnal rest phase and daily activity are suppressed, and standard daily routine does not resume until the next day. At present, an automated image evaluation routine is being applied to the more than 45,000 hours of video streams. Based on this automated approach, a statistical analysis of the ants’ behavior will be carried out. In addition, other parameters (climate, geotectonic and biological), which may influence behavior, will be included in the analysis. PMID:26487310

  18. [Anaerobic digestion of lignocellulosic biomass with animal digestion mechanisms].

    PubMed

    Wu, Hao; Zhang, Pan-Yue; Guo, Jian-Bin; Wu, Yong-Jie

    2013-02-01

    Lignocellulosic material is the most abundant renewable resource in the earth. Herbivores and wood-eating insects are highly effective in the digestion of plant cellulose, while anaerobic digestion process simulating animal alimentary tract still remains inefficient. The digestion mechanisms of herbivores and wood-eating insects and the development of anaerobic digestion processes of lignocellulose were reviewed for better understanding of animal digestion mechanisms and their application in design and operation of the anaerobic digestion reactor. Highly effective digestion of lignocellulosic materials in animal digestive system results from the synergistic effect of various digestive enzymes and a series of physical and biochemical reactions. Microbial fermentation system is strongly supported by powerful pretreatment, such as rumination of ruminants, cellulase catalysis and alkali treatment in digestive tract of wood-eating insects. Oxygen concentration gradient along the digestive tract may stimulate the hydrolytic activity of some microorganisms. In addition, the excellent arrangement of solid retention time, digesta flow and end product discharge enhance the animal digestion of wood cellulose. Although anaerobic digestion processes inoculated with rumen microorganisms based rumen digestion mechanisms were developed to treat lignocellulose, the fermentation was more greatly limited by the environmental conditions in the anaerobic digestion reactors than that in rumen or hindgut. Therefore, the anaerobic digestion processes simulating animal digestion mechanisms can effectively enhance the degradation of wood cellulose and other organic solid wastes.

  19. Archival policies and collections database for the Woods Hole Science Center's marine sediment samples

    USGS Publications Warehouse

    Buczkowski, Brian J.; Kelsey, Sarah A.

    2007-01-01

    The Woods Hole Science Center of the U.S. Geological Survey (USGS) has been an active member of the Woods Hole research community, Woods Hole, Massachusetts, for over 40 years. In that time there have been many projects that involved the collection of sediment samples conducted by USGS scientists and technicians for the research and study of seabed environments and processes. These samples were collected at sea or near shore and then brought back to the Woods Hole Science Center (WHSC) for analysis. While at the center, samples are stored in ambient temperature, refrigerated and freezing conditions ranging from +2º Celsius to -18º Celsius, depending on the best mode of preparation for the study being conducted or the duration of storage planned for the samples. Recently, storage methods and available storage space have become a major concern at the WHSC. The core and sediment archive program described herein has been initiated to set standards for the management, methods, and duration of sample storage. A need has arisen to maintain organizational consistency and define storage protocol. This handbook serves as a reference and guide to all parties interested in using and accessing the WHSC's sample archive and also defines all the steps necessary to construct and maintain an organized collection of geological samples. It answers many questions as to the way in which the archive functions.

  20. Antitermitic Activities of Shisham (Dalbergia Sissoo Roxb.) Heartwood Extractives against two Termite Species

    Treesearch

    Barbar Hassan; Mark Mankowski; Grant T. Kirker; Sohail Ahmed; Muhammad Misbah ul Haq

    2016-01-01

    Shisham (Dalbergia sissoo) heartwood extractives were investigated for antitermitic activities against Heterotermes indicola and Reticulitermes flavipes. Heartwood extractives were removed from wood shavings by soxhlet extraction using (2:1) ethanol: toluene as the solvent system. Filter paper bioassays...

  1. [The influence of oil heat treatment on wood decay resistance by Fourier infrared spectrum analysis].

    PubMed

    Wang, Ya-Mei; Ma, Shu-Ling; Feng, Li-Qun

    2014-03-01

    Wood preservative treatment can improve defects of plantation wood such as easy to corrupt and moth eaten. Among them heat-treatment is not only environmental and no pollution, also can improve the corrosion resistance and dimension stability of wood. In this test Poplar and Mongolian Seoteh Pine was treated by soybean oil as heat-conducting medium, and the heat treatment wood was studied for indoor decay resistance; wood chemical components before and after treatment, the effect of heat treatment on wood decay resistance performance and main mechanism of action were analysed by Fourier infrared spectrometric. Results showed that the mass loss rate of poplar fell from 19.37% to 5% and Mongolian Seoteh Pine's fell from 8.23% to 3.15%, so oil heat treatment can effectively improve the decay resistance. Infrared spectrum analysis shows that the heat treatment made wood's hydrophilic groups such as hydroxyl groups in largely reduced, absorbing capacity decreased and the moisture of wood rotting fungi necessary was reduced; during the heat treatment wood chemical components such as cellulose, hemicellu lose were degraded, and the nutrient source of wood rotting fungi growth necessary was reduced. Wood decay fungi can grow in the wood to discredit wood is because of that wood can provide better living conditions for wood decay fungi, such as nutrients, water, oxygen, and so on. The cellulose and hemicellulose in wood is the main nutrition source of wood decay fungi. So the oil heat-treatment can reduce the cellulose, hemicellulose nutrition source of wood decay fungi so as to improve the decay resistance of wood.

  2. The Environmental Impacts of Boating; Proceedings of a Workshop held at Woods Hole Oceanographic Institution, Woods Hole MA USA, December 7 to 9, 1994.

    DTIC Science & Technology

    1998-03-01

    workshop was the revelation of the greater activity and knowledge base of many state-level environmental managers than that for members of the academic...discussion and get as knowledgeable as we can. And then we can use the information to educate the public to get them concerned and help them understand...court. As I have observed from airplanes, jet skiers run in shallow water looking for animals. They stop near turtles or large sharks in shallow water

  3. Adjustment capacity of maritime pine cambial activity in drought-prone environments.

    PubMed

    Vieira, Joana; Campelo, Filipe; Rossi, Sergio; Carvalho, Ana; Freitas, Helena; Nabais, Cristina

    2015-01-01

    Intra-annual density fluctuations (IADFs) are anatomical features formed in response to changes in the environmental conditions within the growing season. These anatomical features are commonly observed in Mediterranean pines, being more frequent in younger and wider tree rings. However, the process behind IADF formation is still unknown. Weekly monitoring of cambial activity and wood formation would fill this void. Although studies describing cambial activity and wood formation have become frequent, this knowledge is still fragmentary in the Mediterranean region. Here we present data from the monitoring of cambial activity and wood formation in two diameter classes of maritime pine (Pinus pinaster Ait.), over two years, in order to test: (i) whether the differences in stem diameter in an even-aged stand were due to timings and/or rates of xylogenesis; (ii) if IADFs were more common in large trees; and (iii) if their formation is triggered by cambial resumption after the summer drought. Larger trees showed higher rates of cell production and longer growing seasons, due to an earlier start and later end of xylogenesis. When a drier winter occurs, larger trees were more affected, probably limiting xylogenesis in the summer months. In both diameter classes a latewood IADF was formed in 2012 in response to late-September precipitation, confirming that the timing of the precipitation event after the summer drought is crucial in determining the resumption of cambial activity and whether or not an IADF is formed. It was the first time that the formation of a latewood IADF was monitored at a weekly time scale in maritime pine. The capacity of maritime pine to adjust cambial activity to the current environmental conditions represents a valuable strategy under the future climate change conditions.

  4. Exploring Strategies That Influence Children's Physical Activity Self-Efficacy

    ERIC Educational Resources Information Center

    Efrat, Merav W.

    2017-01-01

    Insufficient physical activity during the elementary school years has been associated with a number of health problems (Strong et al., 2006). During the school day, the recess period provides the greatest opportunity for children to engage in physical activity (Robert Wood Johnson, 2007). Nonetheless, most children spend the majority of their…

  5. Essential Oil Variability and Biological Activities of Tetraclinis articulata (Vahl) Mast. Wood According to the Extraction Time.

    PubMed

    Djouahri, Abderrahmane; Saka, Boualem; Boudarene, Lynda; Baaliouamer, Aoumeur

    2016-12-01

    In the present work, the hydrodistillation (HD) and microwave-assisted hydrodistillation (MAHD) kinetics of essential oil (EO) extracted from Tetraclinis articulata (Vahl) Mast. wood was conducted, in order to assess the impact of extraction time and technique on chemical composition and biological activities. Gas chromatography (GC) and GC/mass spectrometry analyses showed significant differences between the extracted EOs, where each family class or component presents a specific kinetic according to extraction time, technique and especially for the major components: camphene, linalool, cedrol, carvacrol and α-acorenol. Furthermore, our findings showed a high variability for both antioxidant and anti-inflammatory activities, where each activity has a specific effect according to extraction time and technique. The highlighted variability reflects the high impact of extraction time and technique on chemical composition and biological activities, which led to conclude that we should select EOs to be investigated carefully depending on extraction time and technique, in order to isolate the bioactive components or to have the best quality of EO in terms of biological activities and preventive effects in food. © 2016 Wiley-VHCA AG, Zurich, Switzerland.

  6. Uses of the Drupal CMS Collaborative Framework in the Woods Hole Scientific Community (Invited)

    NASA Astrophysics Data System (ADS)

    Maffei, A. R.; Chandler, C. L.; Work, T. T.; Shorthouse, D.; Furfey, J.; Miller, H.

    2010-12-01

    Organizations that comprise the Woods Hole scientific community (Woods Hole Oceanographic Institution, Marine Biological Laboratory, USGS Woods Hole Coastal and Marine Science Center, Woods Hole Research Center, NOAA NMFS Northeast Fisheries Science Center, SEA Education Association) have a long history of collaborative activity regarding computing, computer network and information technologies that support common, inter-disciplinary science needs. Over the past several years there has been growing interest in the use of the Drupal Content Management System (CMS) playing a variety of roles in support of research projects resident at several of these organizations. Many of these projects are part of science programs that are national and international in scope. Here we survey the current uses of Drupal within the Woods Hole scientific community and examine reasons it has been adopted. The promise of emerging semantic features in the Drupal framework is examined and projections of how pre-existing Drupal-based websites might benefit are made. Closer examination of Drupal software design exposes it as more than simply a content management system. The flexibility of its architecture; the power of its taxonomy module; the care taken in nurturing the open-source developer community that surrounds it (including organized and often well-attended code sprints); the ability to bind emerging software technologies as Drupal modules; the careful selection process used in adopting core functionality; multi-site hosting and cross-site deployment of updates and a recent trend towards development of use-case inspired Drupal distributions casts Drupal as a general-purpose application deployment framework. Recent work in the semantic arena casts Drupal as an emerging RDF framework as well. Examples of roles played by Drupal-based websites within the Woods Hole scientific community that will be discussed include: science data metadata database, organization main website, biological taxonomy development, bibliographic database, physical media data archive inventory manager, disaster-response website development framework, science project task management, science conference planning, and spreadsheet-to-database converter.

  7. Toxicity of wood smoke particles in human A549 lung epithelial cells: the role of PAHs, soot and zinc.

    PubMed

    Dilger, Marco; Orasche, Jürgen; Zimmermann, Ralf; Paur, Hanns-Rudolf; Diabaté, Silvia; Weiss, Carsten

    2016-12-01

    Indoor air pollution is associated with increased morbidity and mortality. Specifically, the health impact of emissions from domestic burning of biomass and coal is most relevant and is estimated to contribute to over 4 million premature deaths per year worldwide. Wood is the main fuel source for biomass combustion and the shift towards renewable energy sources will further increase emissions from wood combustion even in developed countries. However, little is known about the constituents of wood smoke and biological mechanisms that are responsible for adverse health effects. We exposed A549 lung epithelial cells to collected wood smoke particles and found an increase in cellular reactive oxygen species as well as a response to bioavailable polycyclic aromatic hydrocarbons. In contrast, cell vitality and regulation of the pro-inflammatory cytokine interleukin-8 were not affected. Using a candidate approach, we could recapitulate WSP toxicity by the combined actions of its constituents soot, metals and PAHs. The soot fraction and metals were found to be the most important factors for ROS formation, whereas the PAH response can be mimicked by the model PAH benzo[a]pyrene. Strikingly, PAHs adsorbed to WSPs were even more potent in activating target gene expression than B[a]P individually applied in suspension. As PAHs initiate multiple adverse outcome pathways and are prominent carcinogens, their role as key pollutants in wood smoke and its health effects warrants further investigation. The presented results suggest that each of the investigated constituents soot, metals and PAHs are major contributors to WSP toxicity. Mitigation strategies to prevent adverse health effects of wood combustion should therefore not only aim at reducing the emitted soot and PAHs but also the metal content, through the use of more efficient combustion appliances, and particle precipitation techniques, respectively.

  8. Heavy Metal-Free Tannin from Bark for Sustainable Energy Storage.

    PubMed

    Mukhopadhyay, Alolika; Jiao, Yucong; Katahira, Rui; Ciesielski, Peter N; Himmel, Michael; Zhu, Hongli

    2017-12-13

    A novel renewable cathode made from earth abundant, low-cost materials can contribute to the intermittent storage needs of renewable energy-based society. In this work, we report for the first-time tannin from Nature as a cathode material. Our approach exploits the charge storage mechanism of the redox active quinone moiety. Tannins extracted from tree bark using environmental friendly aqueous solvents have the highest phenol content (5.56 mol g -1 ) among all the natural phenolic biopolymers, 5000 times higher than lignin. Tannins coupled with a conductive polymer polypyrrole acquire high specific capacitance values of 370 F g -1 at 0.5 A g -1 as well as excellent rate performance of 196 F g -1 at 25 A g -1 . Additionally, we employed carbonized wood as an electrode substrate to produce a sustainable electrochemical device with dramatically improved performance compared to conventional devices. The high surface area provided by the well-aligned, cellular porosity of wood-derived substrate combined with the high mobility of ions and electrons in the carbonized cell walls and deposited tannin can achieve an areal capacitance of 4.6 F cm -2 at 1 mA cm -2 , which is 1.5 times higher than activated wood carbon.

  9. 2,4-D adsorption to biochars: effect of preparation conditions on equilibrium adsorption capacity and comparison with commercial activated carbon literature data.

    PubMed

    Kearns, J P; Wellborn, L S; Summers, R S; Knappe, D R U

    2014-10-01

    Batch isotherm experiments were conducted with chars to study adsorption of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Chars generated from corncobs, bamboo and wood chips in a laboratory pyrolyzer at 400-700 °C were compared with traditional kiln charcoals collected from villages in S/SE Asia and with activated carbons (ACs). 2,4-D uptake by laboratory chars obtained from bamboo and wood chips after 14 h of pyrolysis at 700 °C, from wood chips after 96 h of pyrolysis at 600 °C, and one of the field-collected chars (basudha) was comparable to ACs. H:C and O:C ratios declined with pyrolysis temperature and duration while surface area increased to >500 m(2)/g. Increasing pyrolysis intensity by increasing temperature and/or duration of heating was found to positively influence adsorption capacity yield (mg(2,4-D/g(feedstock))) over the range of conditions studied. Economic analysis showed that high temperature chars can be a cost-effective alternative to ACs for water treatment applications. Copyright © 2014 Elsevier Ltd. All rights reserved.

  10. 'Not all that burns is wood'. A social perspective on fuel exploitation and use during the Indus urban period (2600-1900 BC).

    PubMed

    Lancelotti, Carla

    2018-01-01

    Ancient civilisations depended heavily on natural fuel resources for a wide array of activities, and this had an impact on such resources that can be traced in the archaeological record. At its urban apex, the populations of the Indus Civilisation (2600-1900 BC) produced a wide range of objects and crafts, several of which involved highly specialised pyrotechnology. In the wake of increasing aridity and a period of weakened monsoon rainfall that affected South Asia from 2100 BC, these activities potentially put pressure on the natural resource base that may have had to be counterbalanced by differentiation in fuel use. The combined analysis of archaeobotanical and geoarchaeological remains from four Indus urban phase archaeological sites, has enable an assessment of the mechanisms through which people exploited wood, and diversified their fuel resources to adapt to the arid to semi-arid environments in which they lived. The combined use of local wood species with alternative fuels, such as dung and crop-processing leftovers, are evidence for resilient socio-ecological practices during the 700 years of Indus urbanism and perhaps beyond.

  11. 'Not all that burns is wood'. A social perspective on fuel exploitation and use during the Indus urban period (2600-1900 BC)

    PubMed Central

    2018-01-01

    Ancient civilisations depended heavily on natural fuel resources for a wide array of activities, and this had an impact on such resources that can be traced in the archaeological record. At its urban apex, the populations of the Indus Civilisation (2600–1900 BC) produced a wide range of objects and crafts, several of which involved highly specialised pyrotechnology. In the wake of increasing aridity and a period of weakened monsoon rainfall that affected South Asia from 2100 BC, these activities potentially put pressure on the natural resource base that may have had to be counterbalanced by differentiation in fuel use. The combined analysis of archaeobotanical and geoarchaeological remains from four Indus urban phase archaeological sites, has enable an assessment of the mechanisms through which people exploited wood, and diversified their fuel resources to adapt to the arid to semi-arid environments in which they lived. The combined use of local wood species with alternative fuels, such as dung and crop-processing leftovers, are evidence for resilient socio-ecological practices during the 700 years of Indus urbanism and perhaps beyond. PMID:29513672

  12. Antifungal activities of three supercritical fluid extracted cedar oils

    Treesearch

    Tianchuan Du; Todd F. Shupe; Chung Y. Hse

    2009-01-01

    The antifungal activities of three supercritical CO2 (SCC) extracted cedar oils, Port-Orford-cedar (POC) (Chamaecyparis lawsoniana), Alaska yellow cedar (AYC) (Chamaecyparis nootkatensis), and Eastern red cedar (ERC) (Juniperus virginiana L), were evaluated against two common wood decay fungi, brown-rot fungi (...

  13. Co-composting of organic fraction of municipal solid waste mixed with different bulking waste: characterization of physicochemical parameters and microbial enzymatic dynamic.

    PubMed

    Awasthi, Mukesh Kumar; Pandey, Akhilesh Kumar; Bundela, Pushpendra Singh; Khan, Jamaluddin

    2015-04-01

    The effect of various bulking waste such as wood shaving, agricultural and yard trimming waste combined with organic fraction of municipal solid waste (OFMSW) composting was investigated through assessing their influence on microbial enzymatic activities and quality of finished compost. All three piles of OFMSW with different bulking waste were inoculated with microbial consortium. The results revealed that OFMSW combined with wood shaving and microbial consortium (Phanerochaete chrysosporium, Trichoderma viride and Pseudomonas aeruginosa) were helpful tool to facilitate the enzymatic activity and shortened composting period within 4 weeks. Maximum enzymatic activity were observed in pile 1 and 3 during the first 3 weeks, while in pile 2 relatively very low. But phosphatase activity was relatively higher in all piles until the end of the process. Maturity parameters of compost quality also favored the pile 1 as the best formulation for OFMSW composting. Copyright © 2015 Elsevier Ltd. All rights reserved.

  14. Comparison of ultrasonic with stirrer performance for removal of sunset yellow (SY) by activated carbon prepared from wood of orange tree: artificial neural network modeling.

    PubMed

    Ghaedi, A M; Ghaedi, M; Karami, P

    2015-03-05

    The present work focused on the removal of sunset yellow (SY) dye from aqueous solution by ultrasound-assisted adsorption and stirrer by activated carbon prepared from wood of an orange tree. Also, the artificial neural network (ANN) model was used for predicting removal (%) of SY dye based on experimental data. In this study a green approach was described for the synthesis of activated carbon prepared from wood of an orange tree and usability of it for the removal of sunset yellow. This material was characterized using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The impact of variables, including initial dye concentration (mg/L), pH, adsorbent dosage (g), sonication time (min) and temperature (°C) on SY removal were studied. Fitting the experimental equilibrium data of different isotherm models such as Langmuir, Freundlich, Temkin and Dubinin-Radushkevich models display the suitability and applicability of the Langmuir model. Analysis of experimental adsorption data by different kinetic models including pseudo-first and second order, Elovich and intraparticle diffusion models indicate the applicability of the second-order equation model. The adsorbent (0.5g) is applicable for successful removal of SY (>98%) in short time (10min) under ultrasound condition. Copyright © 2014 Elsevier B.V. All rights reserved.

  15. Acoustic and adsorption properties of submerged wood

    NASA Astrophysics Data System (ADS)

    Hilde, Calvin Patrick

    Wood is a common material for the manufacture of many products. Submerged wood, in particular, is used in niche markets, such as the creation of musical instruments. An initial study performed on submerged wood from Ootsa Lake, British Columbia, provided results that showed that the wood was not suitable for musical instruments. This thesis re-examined the submerged wood samples. After allowing the wood to age unabated in a laboratory setting, the wood was retested under the hypothesis that the physical acoustic characteristics would improve. It was shown, however, that the acoustic properties became less adequate after being left to sit. The adsorption properties of the submerged wood were examined to show that the submerged wood had a larger accessible area of wood than that of control wood samples. This implied a lower amount of crystalline area within the submerged wood. From the combined adsorption and acoustic data for the submerged wood, relationships between the moisture content and speed of sound were created and combined with previous research to create a proposed model to describe how the speed of sound varies with temperature, moisture content and the moisture content corresponding to complete hydration of sorption sites within the wood.

  16. Wood as an adherend

    Treesearch

    Bryan H. River; Charles B. Vick; Robert H. Gillespie

    1991-01-01

    Wood is a porous, permeable, hygroscopic, orthotropic, biological composite material of extreme chemical diversity and physical intricacy. Table 1.1 provides an overview of the may variables, including wood variables, that bear on the bonding and performance of wood in wood joints and wood-based materials. Of particular note is the fact that wood properties vary...

  17. 3-D Art Tasks.

    ERIC Educational Resources Information Center

    Niswander, Virginia

    1983-01-01

    Perceptual motor dysfunctions may not allow children with learning and behavior problems to perform as most children do. A successful art activity for these children is construction using wood scraps. (SR)

  18. The influence of temperature, relative humidity and rainfall on the occurrence of the Pine Wood Nematode in Góis Council, Portugal

    NASA Astrophysics Data System (ADS)

    Costa, M. E. G.; Rodrigues, M. A.

    2012-04-01

    In this work, we propose to investigate the influence of the pine wood nematode in the species pinus pinaster in Góis council and the way it affects the economic activity in this region. In order to do that we are going to analyse the influence of temperature, relative humidity and rainfall in the development of the vector insect of the pine wood nematode. In a first stage we are going to do a homogenisation of the series of temperature and rainfall, since they present a significant lack of data. For that we have chosen a reference station that allows us to determine the correlation coefficient to eliminate the lacks that are present in the other series. After that we are going to do the correlation with the number of nematode episodes that occurred and evaluate the area affected for a single year.

  19. Injuries and illnesses from wood framing in residential construction, Washington State, 1993-1999.

    PubMed

    Shah, Syed Mahboob Ali; Bonauto, David; Silverstein, Barbara; Foley, Michael; Kalat, John

    2003-11-01

    The construction industry is associated with high rates of work-related injury. We used workers compensation data to describe the injuries and illnesses, claim rates, and claim costs associated with wood framing activities in construction. From 1993 to 1999, there were 33,021 accepted state fund workers compensation claims with direct costs of over $197 million. The average annual claim rate was 45 per 100 full-time equivalent. Statistically significant downward trends were noted in claim rates for all injuries and illnesses, compensable time loss claims, eye and fall injuries. However, these trends were not statistically significantly different from those observed in all other construction risk classes combined. The information in this report can be used to guide prevention efforts and to evaluate the effectiveness of Washington state initiatives to reduce injury and illness rates in wood frame construction.

  20. Cord Wood Testing in a Non-Catalytic Wood Stove

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Butcher, T.; Trojanowski, R.; Wei, G.

    EPA Method 28 and the current wood stove regulations have been in-place since 1988. Recently, EPA proposed an update to the existing NSPS for wood stove regulations which includes a plan to transition from the current crib wood fuel to cord wood fuel for certification testing. Cord wood is seen as generally more representative of field conditions while the crib wood is seen as more repeatable. In any change of certification test fuel, there are questions about the impact on measured results and the correlation between tests with the two different fuels. The purpose of the work reported here ismore » to provide data on the performance of a noncatalytic stove with cord wood. The stove selected has previously been certified with crib wood which provides a basis for comparison with cord wood. Overall, particulate emissions were found to be considerably higher with cord wood.« less

  1. Foraging ecology of the endangered wood stork recorded in the stable isotope signature of feathers.

    PubMed

    Romanek, C S; Gaines, K F; Bryan, A L; Brisbin, I L

    2000-12-01

    Down feathers and regurgitant were collected from nestling wood storks (Mycteria americana) from two inland and two coastal breeding colonies in Georgia. The stable isotopic ratios of carbon ( 13 C/ 12 C) and nitrogen ( 15 N/ 14 N) in these materials were analyzed to gain insights into the natal origins of juvenile storks and the foraging activities of adults. Down feathers differed in δ 13 C between inland and coastal colonies, having average isotopic values that reflected the sources of carbon fixed in biomass at the base of the food web. Feathers from the inland colonies differed between colonies in δ 15 N, while those from the coastal colonies did not. These patterns primarily reflected the foraging activities of parent storks, with individuals capturing differing percentages of prey of distinct trophic status at each colony. Collectively, the carbon and nitrogen isotopic signatures of feather keratin were used to distinguish nestlings from each colony, except for instances where storks from different colonies foraged in common wetlands. The stable isotopic composition of food items in regurgitant was used to reconstruct the trophic structure of the ecosystems in which wood storks foraged. Predicted foraging activities based on the isotopic composition of keratin were generally consistent with the percentage of prey types (freshwater vs. saltwater and lower trophic level vs. upper trophic level consumer) observed in regurgitant, except for the coastal colony at St. Simons Island, where the δ 13 C of feathers strongly suggested that freshwater prey were a significant component of the diet. This inconsistency was resolved by aerial tracking of adults during foraging excursions using a fixed-wing aircraft. Observed foraging activities supported interpretations based on the stable isotope content of feathers, suggesting that the latter provided a better record of overall foraging activity than regurgitant analysis alone. Observed foraging patterns were compared to the predictions of a statistical model that determined habitat utilization based on habitat availability using a geographic information system (GIS) database. Observed foraging activities and those predicted from feathers both suggested that some adult storks preferred to feed their young freshwater prey, even when saltwater resources were more accessible in the local environment. This conclusion supports the contention that wood stork populations are sensitive to changes in the distribution of freshwater habitats along the southeastern coastal plain of the United States.

  2. Bacterial associations with decaying wood : a review

    Treesearch

    C. A. Clausen

    1996-01-01

    Wood-inhabiting bacteria are associated with wood decay and may have an indirect influence on the decay process. Bacteria are able to affect wood permeability, attack wood structure, or work synergistically with other bacteria and soft-rot fungi to predispose wood to fungal attack. Bacteria that can inhabit chemically treated wood are recognized. The natural ability of...

  3. Synthesis and cure kinetics of liquefied wood/phenol/formaldehyde resins

    Treesearch

    Hui Pan; Todd F. Shupe; Chung-Yun Hse

    2008-01-01

    Wood liquefaction was conducted at a 2/1 phenol/wood ratio in two different reactors: (1) an atmospheric three-necked flask reactor and (2) a sealed Parr reactor. The liquefied wood mixture (liquefied wood, unreacted phenol, and wood residue) was further condensed with formaldehyde under acidic conditions to synthesize two novolac-type liquefied wood/phenol/...

  4. Antibacterial and antifungal activities of pyroligneous acid from wood of Eucalyptus urograndis and Mimosa tenuiflora.

    PubMed

    de Souza Araújo, E; Pimenta, A S; Feijó, F M C; Castro, R V O; Fasciotti, M; Monteiro, T V C; de Lima, K M G

    2018-01-01

    This work aimed to evaluate the antibacterial and antifungal activities of two types of pyroligneous acid (PA) obtained from slow pyrolysis of wood of Mimosa tenuiflora and of a hybrid of Eucalyptus urophylla × Eucalyptus grandis. Wood wedges were carbonized on a heating rate of 1·25°C min -1 until 450°C. Pyrolysis smoke was trapped and condensed to yield liquid products. Crude pyrolysis liquids were bidistilled under 5 mmHg vacuum yielding purified PA. Multi-antibiotic-resistant strains of Escherichia coli, Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923) had their sensitivity to PA evaluated using agar diffusion test. Two yeasts were evaluated as well, Candida albicans (ATCC 10231) and Cryptococcus neoformans. GC-MS analysis of both PAs was carried out to obtain their chemical composition. Regression analysis was performed, and models were adjusted, with diameter of inhibition halos and PA concentration (100, 50 and 20%) as parameters. Identity of regression models and equality of parameters in polynomial orthogonal equations were verified. Inhibition halos were observed in the range 15-25 mm of diameter. All micro-organisms were inhibited by both types of PA even in the lowest concentration of 20%. The feasibility of the usage of PAs produced with wood species planted in large scale in Brazil was evident and the real potential as a basis to produce natural antibacterial and antifungal agents, with real possibility to be used in veterinary and zootechnical applications. © 2017 The Society for Applied Microbiology.

  5. Biogeography of Wood-Boring Crustaceans (Isopoda: Limnoriidae) Established in European Coastal Waters

    PubMed Central

    Borges, Luísa M. S.; Merckelbach, Lucas M.; Cragg, Simon M.

    2014-01-01

    Marine wood-borers of the Limnoriidae cause great destruction to wooden structures exposed in the marine environment. In this study we collated occurrence data obtained from field surveys, spanning over a period of 10 years, and from an extensive literature review. We aimed to determine which wood-boring limnoriid species are established in European coastal waters; to map their past and recent distribution in Europe in order to infer species range extension or contraction; to determine species environmental requirements using climatic envelopes. Of the six species of wood-boring Limnoria previously reported occurring in Europe, only Limnoria lignorum, L. quadripunctata and L. tripunctata are established in European coastal waters. L. carinata and L. tuberculata have uncertain established status, whereas L. borealis is not established in European waters. The species with the widest distribution in Europe is Limnoria lignorum, which is also the most tolerant species to a range of salinities. L. quadripunctata and L. tripunctata appear to be stenohaline. However, the present study shows that both L. quadripunctata and L. tripunctata are more widespread in Europe than previous reports suggested. Both species have been found occurring in Europe since they were described, and their increased distribution is probably the results of a range expansion. On the other hand L. lignorum appears to be retreating poleward with ocean warming. In certain areas (e.g. southern England, and southern Portugal), limnoriids appear to be very abundant and their activity is rivalling that of teredinids. Therefore, it is important to monitor the distribution and destructive activity of these organisms in Europe. PMID:25313796

  6. Biogeography of wood-boring crustaceans (Isopoda: Limnoriidae) established in European coastal waters.

    PubMed

    Borges, Luísa M S; Merckelbach, Lucas M; Cragg, Simon M

    2014-01-01

    Marine wood-borers of the Limnoriidae cause great destruction to wooden structures exposed in the marine environment. In this study we collated occurrence data obtained from field surveys, spanning over a period of 10 years, and from an extensive literature review. We aimed to determine which wood-boring limnoriid species are established in European coastal waters; to map their past and recent distribution in Europe in order to infer species range extension or contraction; to determine species environmental requirements using climatic envelopes. Of the six species of wood-boring Limnoria previously reported occurring in Europe, only Limnoria lignorum, L. quadripunctata and L. tripunctata are established in European coastal waters. L. carinata and L. tuberculata have uncertain established status, whereas L. borealis is not established in European waters. The species with the widest distribution in Europe is Limnoria lignorum, which is also the most tolerant species to a range of salinities. L. quadripunctata and L. tripunctata appear to be stenohaline. However, the present study shows that both L. quadripunctata and L. tripunctata are more widespread in Europe than previous reports suggested. Both species have been found occurring in Europe since they were described, and their increased distribution is probably the results of a range expansion. On the other hand L. lignorum appears to be retreating poleward with ocean warming. In certain areas (e.g. southern England, and southern Portugal), limnoriids appear to be very abundant and their activity is rivalling that of teredinids. Therefore, it is important to monitor the distribution and destructive activity of these organisms in Europe.

  7. Multicycle Wilhelmy plate method for wetting properties, swelling and liquid sorption of wood.

    PubMed

    Moghaddam, Maziar Sedighi; Wålinder, Magnus E P; Claesson, Per M; Swerin, Agne

    2013-10-01

    A multicycle Wilhelmy plate method has been developed to investigate wetting properties, liquid sorption, and swelling of porous substrates such as wood. The use of the method is exemplified by studies of wood veneers of Scots pine sapwood and heartwood, which were subjected to repeated immersion and withdrawal in a swelling liquid (water) and in a nonswelling liquid (octane). The swelling liquid changes the sample dimensions during measurements, in particular its perimeter. This, in turn, influences the force registered. A model based on a linear combination of the measured force and final change in sample perimeter is suggested, and validated to elucidate the dynamic perimeter change of wood veneer samples. We show that pine heartwood and pine sapwood differ in several respects in their interaction with water. Pine heartwood showed (i) lower liquid uptake, (ii) lower swelling, (iii) higher contact angle, and (iv) lower level of dissolution of surface active components (extractives) than pine sapwood. We conclude that the method is also suitable for studying wetting properties of other porous and swellable materials. The wettability results were supported by surface chemical analysis using X-ray photoelectron spectroscopy, showing higher extractives and lignin content on heartwood than on sapwood surfaces.

  8. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Rohay, Alan C.; Clayton, Ray E.; Sweeney, Mark D.

    The Hanford Seismic Assessment Program (HSAP) provides an uninterrupted collection of high-quality raw and processed seismic data from the Hanford Seismic Network for the U.S. Department of Energy and its contractors. The HSAP is responsible for locating and identifying sources of seismic activity and monitoring changes in the historical pattern of seismic activity at the Hanford Site. The data are compiled, archived, and published for use by the Hanford Site for waste management, natural phenomena hazards assessments, and engineering design and construction. In addition, the HSAP works with the Hanford Site Emergency Services Organization to provide assistance in the eventmore » of a significant earthquake on the Hanford Site. The Hanford Seismic Network and the Eastern Washington Regional Network consist of 44 individual sensor sites and 15 radio relay sites maintained by the Hanford Seismic Assessment Team. During FY 2010, the Hanford Seismic Network recorded 873 triggers on the seismometer system, which included 259 seismic events in the southeast Washington area and an additional 324 regional and teleseismic events. There were 210 events determined to be local earthquakes relevant to the Hanford Site. One hundred and fifty-five earthquakes were detected in the vicinity of Wooded Island, located about eight miles north of Richland just west of the Columbia River. The Wooded Island events recorded this fiscal year were a continuation of the swarm events observed during fiscal year 2009 and reported in previous quarterly and annual reports (Rohay et al. 2009a, 2009b, 2009c, 2010a, 2010b, and 2010c). Most events were considered minor (coda-length magnitude [Mc] less than 1.0) with the largest event recorded on February 4, 2010 (3.0Mc). The estimated depths of the Wooded Island events are shallow (averaging approximately 1.5 km deep) placing the swarm within the Columbia River Basalt Group. Based upon the last two quarters (Q3 and Q4) data, activity at the Wooded Island area swarm has largely subsided. Pacific Northwest National Laboratory will continue to monitor for activity at this location. The highest-magnitude events (3.0Mc) were recorded on February 4, 2010 within the Wooded Island swarm (depth 2.4 km) and May 8, 2010 on or near the Saddle Mountain anticline (depth 3.0 km). This latter event is not considered unusual in that earthquakes have been previously recorded at this location, for example, in October 2006 (Rohay et al. 2007). With regard to the depth distribution, 173 earthquakes were located at shallow depths (less than 4 km, most likely in the Columbia River basalts), 18 earthquakes were located at intermediate depths (between 4 and 9 km, most likely in the pre-basalt sediments), and 19 earthquakes were located at depths greater than 9 km, within the crystalline basement. Geographically, 178 earthquakes were located in known swarm areas, 4 earthquakes occurred on or near a geologic structure (Saddle Mountain anticline), and 28 earthquakes were classified as random events. The Hanford Strong Motion Accelerometer (SMA) network was triggered several times by the Wooded Island swarm events and the events located on or near the Saddle Mountain anticline. The maximum acceleration value recorded by the SMA network during fiscal year 2010 occurred February 4, 2010 (Wooded Island swarm event), approximately 2 times lower than the reportable action level for Hanford facilities (2% g) with no action required.« less

  9. New species of Ipomoea (Convolvulaceae) from South America

    PubMed Central

    Wood, John R.I.; Muñoz-Rodríguez, Pablo; Degen, Rosa; Scotland, Robert W.

    2017-01-01

    Abstract The importance of discovering, describing and cataloguing poorly known species in herbarium collections is discussed. It is a spur to efforts at rediscovery and consequent conservation efforts. The problems faced in describing species from limited material are discussed and our methods and criteria in making a decision are described. Prospects for future novelties are briefly assessed. Fifteen new species are described and illustrated with line drawings and distribution maps: Ipomoea attenuata J.R.I. Wood & Scotland, I. cuscoensis J.R.I. Wood & P. Muñoz, I. dasycarpa J.R.I. Wood & Scotland, I. dolichopoda J.R.I. Wood & R. Degen, I. ensiformis J.R.I.Wood & Scotland, I. fasciculata J.R.I. Wood & Scotland, I. graminifolia J.R.I. Wood & Scotland, I. kraholandica J.R.I. Wood & Scotland, I. longirostra J.R.I. Wood & Scotland, I. revoluta J.R.I. Wood & Scotland, I. scopulina J.R.I. Wood &. Scotland, I. uninervis J.R.I. Wood & Scotland, I. veadeirosii J.R.I. Wood & Scotland, I. velutinifolia J.R.I. Wood & Scotland, I. walteri J.R.I. Wood & Scotland. All species are narrow endemics except I. velutinifolia which is found in Brazil and Peru; of the others, 12 are found in Brazil and one each in Paraguay and Peru. PMID:29118645

  10. Competitive outcomes between wood-decaying fungi are altered in burnt wood.

    PubMed

    Edman, Mattias; Eriksson, Anna-Maria

    2016-06-01

    Fire is an important disturbance agent in boreal forests where it creates a wide variety of charred and other types of heat-modified dead wood substrates, yet how these substrates affect fungal community structure and development within wood is poorly understood. We allowed six species of wood-decaying basidiomycetes to compete in pairs in wood-discs that were experimentally burnt before fungal inoculation. The outcomes of interactions in burnt wood differed from those in unburnt control wood for two species:Antrodia sinuosanever lost on burnt wood and won over its competitor in 67% of the trials compared to 40% losses and 20% wins on unburnt wood. In contrast, Ischnoderma benzoinumwon all interactions on unburnt wood compared to 33% on burnt wood. However, the responses differed depending on the identity of the competing species, suggesting an interaction between competitor and substrate type. The observed shift in competitive balance between fungal species probably results from chemical changes in burnt wood, but the underlying mechanism needs further investigation. Nevertheless, the results indicate that forest fires indirectly structure fungal communities by modifying dead wood, and highlight the importance of fire-affected dead wood substrates in boreal forests. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  11. Forest Community: An Ecological Study

    ERIC Educational Resources Information Center

    Zipko, Stephen J.

    1974-01-01

    Discusses science activities designed to examine a balanced community in an unspoiled wooded section, including the study of physical and floral characteristics, shrubs, small and large trees, and animals. Indicates that the students' awareness of man's place in nature and nature's place in man is enhanced through such activities. (CC)

  12. DETECTION OF ANDROGENIC ACTIVITY IN EMISSIONS FROM DIESEL FUEL AND BIOMASS COMBUSTION

    EPA Science Inventory

    The present study evaluated both diesel fuel exhaust and biomass (wood) burn extracts for androgen receptor¿mediated activity using MDA-kb2 cells, which contain an androgen-responsive promoter-luciferase reporter gene construct. This assay and analytical fractionization of the sa...

  13. Activation of respiratory epithelial cells by wood smoke particles persists beyond immediate exposure.

    EPA Science Inventory

    The biological effect of particles on epithelial cells involves, in part, oxidant generation and a cascade of reactions culminating in inflammatory mediator release. Whether there is an immediate short-lived activation or continued persistent response of the cells to the particle...

  14. Chapter 6: Wood energy and competing wood product markers

    Treesearch

    Kenneth E. Skog; Robert C. Abt; Karen Abt

    2014-01-01

    Understanding the effect of expanding wood energy markets is important to all wood-dependent industries and to policymakers debating the implementation of public programs to support the expansion of wood energy generation. A key factor in determining the feasibility of wood energy projects (e.g. wood boiler or pellet plant) is the long-term (i.e. 20-30year) supply...

  15. Wood flour

    Treesearch

    Craig M. Clemons; Daniel F. Caufield

    2005-01-01

    The term “wood flour” is somewhat ambiguous. Reineke states that the term wood flour “is applied somewhat loosely to wood reduced to finely divided particles approximating those of cereal flours in size, appearance, and texture”. Though its definition is imprecise, the term wood flour is in common use. Practically speaking, wood flour usually refers to wood particles...

  16. Wood flour

    Treesearch

    Craig M. Clemons

    2010-01-01

    The term “wood flour” is somewhat ambiguous. Reineke states that the term wood flour “is applied somewhat loosely to wood reduced to finely divided particles approximating those of cereal flours in size, appearance, and texture.” Though its definition is imprecise, the term wood flour is in common use. Practically speaking, wood flour usually refers to wood particles...

  17. Patterns and determinants of wood physical and mechanical properties across major tree species in China.

    PubMed

    Zhu, JiangLing; Shi, Yue; Fang, LeQi; Liu, XingE; Ji, ChengJun

    2015-06-01

    The physical and mechanical properties of wood affect the growth and development of trees, and also act as the main criteria when determining wood usage. Our understanding on patterns and controls of wood physical and mechanical properties could provide benefits for forestry management and bases for wood application and forest tree breeding. However, current studies on wood properties mainly focus on wood density and ignore other wood physical properties. In this study, we established a comprehensive database of wood physical properties across major tree species in China. Based on this database, we explored spatial patterns and driving factors of wood properties across major tree species in China. Our results showed that (i) compared with wood density, air-dried density, tangential shrinkage coefficient and resilience provide more accuracy and higher explanation power when used as the evaluation index of wood physical properties. (ii) Among life form, climatic and edaphic variables, life form is the dominant factor shaping spatial patterns of wood physical properties, climatic factors the next, and edaphic factors have the least effects, suggesting that the effects of climatic factors on spatial variations of wood properties are indirectly induced by their effects on species distribution.

  18. Effects of humidity on the magnetic and woody characteristics of powder-type magnetic wood

    NASA Astrophysics Data System (ADS)

    Oka, H.; Tokuta, H.; Namizaki, Y.; Sekino, N.

    2004-05-01

    Among three types of proposed magnetic wood, powder-type magnetic wood can be made of recycled magnetic materials from IT devices, consumer electronics and waste wood. Because of its wood powder content, powder-type magnetic wood shows special characteristics different from those of typical magnetic materials. We focused on the relationship between humidity and magnetic characteristics of powder-type magnetic wood. The magnetic powder ratio, wood powder density and magnetic binder density were all examined as parameters for AC permeability.

  19. Study of influence of 2.4 GHz electromagnetic waves on electrophysical properties of coniferous trees wood

    NASA Astrophysics Data System (ADS)

    Abdurahimov, Nursulton; Lagunov, Alexey; Melehov, Vladimir

    2017-09-01

    Climate change has a significant impact on changing weather conditions in the Arctic. Wood is a traditional building material in the North of Russia. Supports of communication lines are made of wood. Dry wood is a solid dielectric with a low conductivity. At the same time it is porous material having high hygroscopicity. The presence of moisture leads to wood rotting. To prevent rotting of a support it needs to be impregnated with antiseptics. A tree dried by means of convection drying cannot provide required porosity of wood for impregnation. Our studies of electrophysical properties of coniferous species showed that microwave drying of wood increases the porosity of the wood. Wood dried in this way is easily impregnated with antiseptics. Thorough wood drying requires creating optimal conditions in a microwave oven. During the drying process in a chamber there is a resonant phenomenon. These phenomena depend on electro-physical properties of the material placed in the chamber. Dielectric constant of wood has the most influence. A resonator method to determine the dielectric constant of the wood was used. The values of permittivity for the spruce and pine samples were determined. The measured value of the dielectric constant of wood was used to provide optimal matching of the generator with the resonator in a wood-drying resonator type microwave chamber, and to maintain it in the process of wood drying. It resulted in obtaining the samples with a higher permeability of wood in radial and longitudinal direction. This creates favorable conditions for wood impregnation with antiseptics and flame retardants. Timber dried by means of electromagnetic waves in the 2.4 GHz band has a deeper protective layer. The support made of such wood will serve longer as supports of communication lines.

  20. Fundamental adsorption characteristics of carbonaceous adsorbents for 1,2,3,4-tetrachlorobenzene in a model gas of an incineration plant.

    PubMed

    Inoue, Kenichiro; Kawamoto, Katsuya

    2005-08-01

    Carbonaceous adsorbents such as activated carbon have been used to reduce the emission of organic pollutants from incineration plants. However, with this method, the amount and type of adsorbent to be used are based only on empirical results, which may lead to overuse of the adsorbents. The fundamental adsorption characteristics of several kinds of activated carbon, activated coke, and carbide wood were examined using 1 ,2,3,4-tetrachlorobenzene as an adsorbate. The removal performance and various equilibrium adsorption characteristics of these adsorbents were analyzed using laboratory-scale adsorption equipment. The equilibrium adsorption amount increased by a factor of 1.9-3.2 at 150 degrees C compared with that at 190 degrees C. The effect of the moisture content on adsorption capacity was relatively small in comparison with that of the temperature. The micropore volume for pore diameters of 2 nm or less was the most important factor governing the adsorption capacity for all adsorbents. Activated carbon showed superior adsorption ability compared to activated coke and carbide wood, although all adsorbents were sufficient for practical use.

  1. FIELD-SCALE LEACHING OF ARSENIC, CHROMIUM AND COPPER FROM WEATHERED TREATED WOOD

    PubMed Central

    Hasan, A. Rasem; Hu, Ligang; Solo-Gabriele, Helena M.; Fieber, Lynne; Cai, Yong; Townsend, Timothy G.

    2010-01-01

    Earlier studies documented the loss of wood preservatives from new wood. The objective of this study was to evaluate losses from weathered treated wood under field conditions by collecting rainfall leachate from 5 different wood types, all with a surface area of 0.21 m2. Wood samples included weathered chromate copper arsenate (CCA) treated wood at low (2.7 kg/m3), medium (4.8 kg/m3) and high (35.4 kg/m3) retention levels, new alkaline copper quat (ACQ) treated wood (1.1 kg/m3 as CuO) and new untreated wood. Arsenic was found to leach at a higher rate (100 mg in 1 year for low retention) than chromium and copper (<40 mg) in all CCA treated wood samples. Copper leached at the highest rate from the ACQ sample (670 mg). Overall results suggest that metals’ leaching is a continuous process driven by rainfall, and that the mechanism of release from the wood matrix changes as wood weathers. PMID:20053493

  2. Evaluation of plant species composition after thirteen years post coal mining rehabilitation in East Kutai District of East Kalimantan, Indonesia

    NASA Astrophysics Data System (ADS)

    Komara, L. L.; Murtinah, V.; Arbain

    2018-04-01

    Coal mining leaving large overburden which needs to be rehabilitated by conducting reclamation and re-vegetation. This study objective was to evaluate plant species composition after thirteen years of re-vegetation activities in a coal mining rehabilitation site in KutaiTimur District, East Kalimantan, with coordinate 00º33’23”-00º38’17” NL and 117º23’55”-117º23’20” EL. This study compared rehabilitation site with the natural forest conditions sites. There were found 28 plant species in the rehabilitation site, consisting of 19 wood species (Cassia siamea and Ficus uncinata) and 9 non-wood species (Nephrolepis biserata and Miscanthus javanica).In comparison, 36 species were found in the natural forest condition, consisting of 25 woody species (Nephelium eriopetalum and Macaranga hypoleuca)and 11 non-wood species (Fordia splendidisima and Saurauia umbellata). Woody species diversity indices in the rehabilitation site after 13 years post mining (i.e., 2,21) was lower than in the natural forest sites (3,01); while the diversity indices for non-wood species were relatively similar (1,48 and 1,96 in the rehabilitation and natural forest sites respectively). Species richness of non-wood species was low, but it has a high coverage per species in this site.To restore rehabilitation site woody species to its assumed natural conditions, 22woody species should be planted.

  3. Life strategies in intra-annual dynamics of wood formation: example of three conifer species in a temperate forest in north-east France.

    PubMed

    Cuny, Henri E; Rathgeber, Cyrille B K; Lebourgeois, François; Fortin, Mathieu; Fournier, Meriem

    2012-05-01

    We investigated whether timing and rate of growth are related to the life strategies and fitness of three conifer species. Intra-annual dynamics of wood formation, shoot elongation and needle phenology were monitored over 3 years in five Norway spruces (Picea abies (L.) Karst.), five Scots pines (Pinus sylvestris L.) and five silver firs (Abies alba Mill.) grown intermixed. For the three species, the growing season (delimited by cambial activity onset and cessation) lasted about 4 months, while the whole process of wood formation lasted 5-6 months. Needle unfolding and shoot elongation followed the onset of cambial activity and lasted only one-third of the season. Pines exhibited an 'extensive strategy' of cambial activity, with long durations but low growth rates, while firs and spruces adopted an 'intensive strategy' with shorter durations but higher growth rates. We estimated that about 75% of the annual radial increment variability was attributable to the rate of cell production, and only 25% to its duration. Cambial activity rates culminated at the same time for the three species, whereas shoot elongation reached its maximal rate earlier in pines. Results show that species-specific life strategies are recognizable through functional traits of intra-annual growth dynamics. The opposition between Scots pine extensive strategy and silver fir and Norway spruce intensive strategy supports the theory that pioneer species are greater resource expenders and develop riskier life strategies to capture resources, while shade-tolerant species utilize resources more efficiently and develop safer life strategies. Despite different strategies, synchronicity of the maximal rates of cambial activity suggests a strong functional convergence between co-existing conifer species, resulting in head-on competition for resources.

  4. Disentangling the drivers of coarse woody debris behavior and carbon gas emissions during fire

    NASA Astrophysics Data System (ADS)

    Zhao, Weiwei; van der Werf, Guido R.; van Logtestijn, Richard S. P.; van Hal, Jurgen R.; Cornelissen, Johannes H. C.

    2016-04-01

    The turnover of coarse woody debris, a key terrestrial carbon pool, plays fundamental roles in global carbon cycling. Biological decomposition and fire are two main fates for dead wood turnover. Compared to slow decomposition, fire rapidly transfers organic carbon from the earth surface to the atmosphere. Both a-biotic environmental factors and biotic wood properties determine coarse wood combustion and thereby its carbon gas emissions during fire. Moisture is a key inhibitory environmental factor for fire. The properties of dead wood strongly affect how it burns either directly or indirectly through interacting with moisture. Coarse wood properties vary between plant species and between various decay stages. Moreover, if we put a piece of dead wood in the context of a forest fuel bed, the soil and wood contact might also greatly affect their fire behavior. Using controlled laboratory burns, we disentangled the effects of all these driving factors: tree species (one gymnosperms needle-leaf species, three angiosperms broad-leaf species), wood decay stages (freshly dead, middle decayed, very strongly decayed), moisture content (air-dried, 30% moisture content in mass), and soil-wood contact (on versus 3cm above the ground surface) on dead wood flammability and carbon gas efflux (CO2 and CO released in grams) during fire. Wood density was measured for all coarse wood samples used in our experiment. We found that compared to other drivers, wood decay stages have predominant positive effects on coarse wood combustion (for wood mass burned, R2=0.72 when air-dried and R2=0.52 at 30% moisture content) and associated carbon gas emissions (for CO2andCO (g) released, R2=0.55 when air-dried and R2=0.42 at 30% moisture content) during fire. Thus, wood decay accelerates wood combustion and its CO2 and CO emissions during fire, which can be mainly attributed to the decreasing wood density (for wood mass burned, R2=0.91 when air-dried and R2=0.63 at 30% moisture content) as wood becomes more decomposed. Our results provide quantitative experimental evidence for how several key abiotic and biotic factors, especially moisture content and the key underlying trait wood density, as well as their interactions, together drive coarse wood carbon turnover through fire. Our experimental data on coarse wood behavior and gas efflux during fire will help to improve the predictive power of global vegetation climate models on dead wood turnover and its feedback to climate.

  5. UV resistance and dimensional stability of wood modified with isopropenyl acetate.

    PubMed

    Nagarajappa, Giridhar B; Pandey, Krishna K

    2016-02-01

    Chemical modification of Rubberwood (Hevea brasiliensis Müll.Arg) with isopropenyl acetate (IPA) in the presence of anhydrous aluminum chloride as a catalyst has been carried out under solvent free conditions. The level of modification was estimated by determining the weight percent gain and modified wood was characterized by FTIR-ATR and CP/MAS (13)C NMR spectroscopy. The effect of catalyst concentration on WPG was studied. UV resistance, moisture adsorption and dimensional stability of modified wood were evaluated. UV resistance of modified wood was evaluated by exposing unmodified and modified wood to UV irradiation in a QUV accelerated weathering tester. Unmodified wood showed rapid color changes and degradation of lignin upon exposure to UV light. Chemical modification of wood polymers with IPA was effective in reducing light induced color changes (photo-yellowing) at wood surfaces. In contrast to unmodified wood, modified wood exhibited bleaching. FTIR analysis of modified wood exposed to UV light indicated stabilization of wood polymers against UV degradation. Modified wood showed good dimensional stability and hydrophobicity. Thermogravimetric analysis showed that modification with IPA improved thermal stability of wood. Improved dimensional stability and UV resistance of modified wood indicates IPA as a promising reagent since there is no acid byproduct of reaction as observed in case of other esterification reactions. Copyright © 2015 Elsevier B.V. All rights reserved.

  6. Effect of Hinoki and Meniki Essential Oils on Human Autonomic Nervous System Activity and Mood States.

    PubMed

    Chen, Chi-Jung; Kumar, K J Senthil; Chen, Yu-Ting; Tsao, Nai-Wen; Chien, Shih-Chang; Chang, Shang-Tzen; Chu, Fang-Hua; Wang, Sheng-Yang

    2015-07-01

    Meniki (Chamecyparis formosensis) and Hinoki (C. obtusa) are precious conifers with excellent wood properties and distinctive fragrances that make these species popular in Taiwan for construction, interiors and furniture. In the present study, the compositions of essential oils prepared from Meniki and Hinoki were analyzed by gas chromatography-mass spectrometry (GC/MS). Thirty-six compounds were identified from the wood essential oil of Meniki, including Δ-cadinene, γ-cadinene, Δ-cadinol, α-muurolene, calamenene, linalyl acetate and myrtenol; 29 compounds were identified from Hinoki, including α-terpineol, α-pinene, Δ-cadinene, borneol, terpinolene, and limonene. Next, we examined the effect of Meniki and Hinoki essential oils on human autonomic nervous system activity. Sixteen healthy adults received Meniki or Hinoki by inhalation for 5 min, and the physiological and psychological effects were examined. After inhaling Meniki essential oil, participant's systolic blood pressure and heart rate (HR) were decreased, and diastolic blood pressure increased. In addition, sympathetic nervous activity (SNS) was significantly decreased, and parasympathetic activity (PSNS) was significantly increased. On the other hand, after inhaling Hinoki essential oil, systolic blood pressure, heart rate and PSNS were decreased, whereas SNA was increased. Indeed, both Meniki and Hinoki essential oils increased heart rate variability (HRV) in tested adults. Furthermore, in the Profile of Mood States (POMS) test, both Meniki and Hinoki wood essential oils stimulated a pleasant mood status. Our results strongly suggest that Meniki and Hinoki essential oils could be suitable agents for the development of regulators of sympathetic nervous system dysfunctions.

  7. 78 FR 730 - State Program Requirements; Approval of Application To Administer Partial National Pollutant...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-04

    ...) at facilities whose primary industrial activity is storage of grain, feed seed, fertilizer, and... incidentally store grain, feed seed, fertilizer, and agriculture chemicals to support the primary activity of..., except those related to wood preservation and processing regulated pursuant to 40 CFR part 429 (Timber...

  8. Sustaining Our Forest Resource: What is Your Role?

    ERIC Educational Resources Information Center

    Osterndorf, Laurie; Piraino, Nancy

    This booklet provides students with challenging and fun activities to encourage learning about the sustainability of natural forest resources. Activities include: (1) "Forest-opoly"; (2) "Lost in the Woods"; (3) "Chain of Changes"; (4) "Choices, Choices"; (5) "Conflicts & Controversies"; (6) "Tree Towns"; (7) "Feeding the Forest"; (8) "Get to the…

  9. Wood fuel preparation

    Treesearch

    L. H. Reineke

    1965-01-01

    This report gives information on the preparation of wood fuel from wood residues and other wood raw materials. Types of wood fuel discussed are cordwood, stovewood, slabwood, kindling, chips, hogged fuel, sawdust and shavings, bark, charcoal, alcohol, and briquets. Related information is given on types of machinery for preparing wood fuel and on possible markets for...

  10. Evaluation of interior and exterior latex paints : final report.

    DOT National Transportation Integrated Search

    1979-10-01

    The wood panels that have only one coat of paint over bare wood and one coat of paint over primed wood are continuing to show sighs of deterioration. The wood panels that have two coats of paint over bare wood and two coats of paint over primed wood ...

  11. Wood Programs. Courseware Evaluation for Vocational and Technical Education.

    ERIC Educational Resources Information Center

    Kaylor, Robert; And Others

    This courseware evaluation rates the Wood Programs software developed by the Iowa Department of Public Instruction. (These programs--not contained in this document--include understanding board feet, wood characteristics, wood safety drill, wood dimensions, wood moisture, operating the table saw, radial arm, measurement drill, fraction drill, and…

  12. 76 FR 76690 - Multilayered Wood Flooring From the People's Republic of China: Amended Final Determination of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-08

    ... DEPARTMENT OF COMMERCE International Trade Administration [A-570-970] Multilayered Wood Flooring... is issuing an antidumping duty order on multilayered wood flooring (``wood flooring'') from the... antidumping duty investigation of wood flooring from the PRC. See Multilayered Wood Flooring From the People's...

  13. Corrosion of metals in wood : comparing the results of a rapid test method with long-term exposure tests across six wood treatments

    Treesearch

    Samuel L. Zelinka; Donald S. Stone

    2011-01-01

    This paper compares two methods of measuring the corrosion of steel and galvanized steel in wood: a long-term exposure test in solid wood and a rapid test method where fasteners are electrochemically polarized in extracts of wood treated with six different treatments. For traditional wood preservatives, the electrochemical extract method correlates with solid wood...

  14. Methanol from wood waste : a technical and economic study

    Treesearch

    A. E. Hokanson; R. M. Rowell

    1977-01-01

    A methanol-from-wood waste facility having a capacity of 50 million gallons per year requires 1,500 ovendry tons (ODT) of wood waste per day. The yield of methanol from wood is about 38 percent, or about 100 gallons per ODT of wood. This yield is based on all process energy required coming from the wood waste. At a wood waste cost of $15/ODT, the selling price of...

  15. The use of new, aqueous chemical wood modifications to improve the durability of wood-plastic composites

    Treesearch

    Rebecca E. Ibach; Craig M. Clemons; George C. Chen

    2017-01-01

    The wood flour used in wood-plastic composites (WPCs) can biologically deteriorate and thus the overall mechanical performance of WPCs decrease when exposed to moisture and fungal decay. Protecting the wood flour by chemical modification can improve the durability of the wood in a nontoxic way so it is not harmful to the environment. WPCs were made with modified wood...

  16. Soil quality in a cropland soil treated with wood ash containing charcoal

    NASA Astrophysics Data System (ADS)

    Omil, Beatriz; Balboa, Miguel A.; Fonturbel, M. Teresa; Gartzia-Bengoetxea, Nahia; Arias-González, Ander; Vega, Jose A.; Merino, Agustin

    2014-05-01

    The strategy of the European Union "Europe 2020" states that by 2020, 20% of final energy consumption must come from renewables. In this scenario, there is an increasing use of biomass utilization for energy production. Indeed, it is expected that the production of wood-ash will increase in coming years. Wood ash, a mixture of ash and charcoal, generated as a by-product of biomass combustion in power plants, can be applied to soil to improve the soil quality and crop production. Since the residue contains significant content of charcoal, the application of mixed wood ash may also improve the SOM content and soil quality in the long term, in soils degraded as a consequence of intensive management. The objective of this study was asses the changes in SOM quality and soil properties in a degraded soils treated with wood ash containing charcoal. The study was carried out in a field devoted to cereal crops during the last decades. The soil was acidic (pH 4.5) with a low SOC content (3 %) and fine texture. The experiment was based on a randomised block design with four replicates. Each block included the following four treatments: Control, 16 Mg fly wood ash ha-1, 16 Mg mixed wood ash ha-1 (16 Mg) and 32 Mg mixed wood ash ha-1 (32 Mg). The application was carried out once. The ash used in the study was obtained from a thermal power plant and was mainly derived from the combustion of Pinus radiata bark and branches. The wood ash is highly alkaline (pH= 10), contains 10 % of highly condensed black carbon (atomic H/C ratio < 0.5 and T50 en DSC= 500 ºC). The evolution of SOM properties were monitored over three years by solid state 13C CPMAS NMR and Differential Scanning Calorimetry (DSC). These techniques were applied in bulk samples and aggregates of different sizes. The changes in microbial activity were studied by analysis of microbial biomass C and basal respiration. The soil bacterial community was studied by the Biolog method. Several physical properties, such soil aggregate distribution, hydraulic conductivity and available water contente were also determined. Three years after applications the SOM content increased lightly in the treatment receiving more than 16 Mg ha-1 of wood ash. SOM in the treated soils displayed a higher degree of aromaticity than in the untreated soils, indicating a gain in more stable SOM compounds probably as a consequence of the charcoal application. However, both methods also revealed increases in labile C compounds, probably due to the carbohydrates added through root system. Microbial biomass-C and soil respiration increased significantly. The treatments also led to increases in the functional diversity indices. The amended soils showed greater utilization of substrates and the ability of soil bacteria to utilize different C resources was also greatly altered. The application of mixed wood ash did not lead to any difference in MWD, which was around 6 mm in all cases. The application of 16 Mg fly wood ash ha-1 increased significantly the hydraulic conductivity (4.07 cmh-1) when compared with in control plots (1.3 cmh-1) and mixed ash plots (1.52 and 2.45 cmh-1, 16 Mg and 32 Mg respectively). However, air-filled porosity was not higher in 16 Mg fly ash plots. AWC was not improved by wood ash application.

  17. Antibacterial activity of Artocarpus heterophyllus.

    PubMed

    Khan, M R; Omoloso, A D; Kihara, M

    2003-07-01

    The crude methanolic extracts of the stem and root barks, stem and root heart-wood, leaves, fruits and seeds of Artocarpus heterophyllus and their subsequent partitioning with petrol, dichloromethane, ethyl acetate and butanol gave fractions that exhibited a broad spectrum of antibacterial activity. The butanol fractions of the root bark and fruits were found to be the most active. None of the fractions were active against the fungi tested.

  18. Thermogravimetric and calorimetric characteristics during co-pyrolysis of municipal solid waste components.

    PubMed

    Ansah, Emmanuel; Wang, Lijun; Shahbazi, Abolghasem

    2016-10-01

    The thermogravimetric and calorimetric characteristics during pyrolysis of wood, paper, textile and polyethylene terephthalate (PET) plastic in municipal solid wastes (MSW), and co-pyrolysis of biomass-derived and plastic components with and without torrefaction were investigated. The active pyrolysis of the PET plastic occurred at a much higher temperature range between 360°C and 480°C than 220-380°C for the biomass derived components. The plastic pyrolyzed at a heating rate of 10°C/min had the highest maximum weight loss rate of 18.5wt%/min occurred at 420°C, followed by 10.8wt%/min at 340°C for both paper and textile, and 9.9wt%/min at 360°C for wood. At the end of the active pyrolysis stage, the final mass of paper, wood, textile and PET was 28.77%, 26.78%, 21.62% and 18.31%, respectively. During pyrolysis of individual MSW components at 500°C, the wood required the least amount of heat at 665.2J/g, compared to 2483.2J/g for textile, 2059.4J/g for paper and 2256.1J/g for PET plastic. The PET plastic had much higher activation energy of 181.86kJ/mol, compared to 41.47kJ/mol for wood, 50.01kJ/mol for paper and 36.65kJ/mol for textile during pyrolysis at a heating rate of 10°C/min. H2O and H2 peaks were observed on the MS curves for the pyrolysis of three biomass-derived materials but there was no obvious H2O and H2 peaks on the MS curves of PET plastic. There was a significant interaction between biomass and PET plastic during co-pyrolysis if the biomass fraction was dominant. The amount of heat required for the co-pyrolysis of the biomass and plastic mixture increased with the increase of plastic mass fraction in the mixture. Torrefaction at a proper temperature and time could improve the grindability of PET plastic. The increase of torrefaction temperature and time did not affect the temperature where the maximum pyrolytic rates occurred for both biomass and plastic but decreased the maximum pyrolysis rate of biomass and increased the maximum pyrolysis rate of PET plastic. The amount of heat for the pyrolysis of biomass and PET mixture co-torrefied at 280°C for 30min was 4365J/g at 500°C, compared to 1138J/g for the pyrolysis of raw 50% wood and 50% PET mixture at the same condition. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. A Novel Slurry-Based Biomass Reforming Process Final Technical Report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Emerson, Sean C.; Davis, Timothy D.; Peles, A.

    2011-09-30

    This project was focused on developing a catalytic means of producing H2 from raw, ground biomass, such as fast growing poplar trees, willow trees, or switch grass. The use of a renewable, biomass feedstock with minimal processing can enable a carbon neutral means of producing H2 in that the carbon dioxide produced from the process can be used in the environment to produce additional biomass. For economically viable production of H2, the biomass is hydrolyzed and then reformed without any additional purification steps. Any unreacted biomass and other byproduct streams are burned to provide process energy. Thus, the development ofmore » a catalyst that can operate in the demanding corrosive environment and presence of potential poisons is vital to this approach. The concept for this project is shown in Figure 1. The initial feed is assumed to be a >5 wt% slurry of ground wood in dilute base, such as potassium carbonate (K2CO3). Base hydrolysis and reforming of the wood is carried out at high but sub-critical pressures and temperatures in the presence of a solid catalyst. A Pd alloy membrane allows the continuous removal of pure , while the retentate, including methane is used as fuel in the plant. The project showed that it is possible to economically produce H2 from woody biomass in a carbon neutral manner. Technoeconomic analyses using HYSYS and the DOE's H2A tool [1] were used to design a 2000 ton day-1 (dry basis) biomass to hydrogen plant with an efficiency of 46% to 56%, depending on the mode of operation and economic assumptions, exceeding the DOE 2012 target of 43%. The cost of producing the hydrogen from such a plant would be in the range of $1/kg H2 to $2/kg H2. By using raw biomass as a feedstock, the cost of producing hydrogen at large biomass consumption rates is more cost effective than steam reforming of hydrocarbons or biomass gasification and can achieve the overall cost goals of the DOE Fuel Cell Technologies Program. The complete conversion of wood to hydrogen, methane, and carbon dioxide was repeatedly demonstrated in batch reactors varying in size from 50 mL to 7.6 L. The different wood sources (e.g., swamp maple, poplar, and commercial wood flour) were converted in the presence of a heterogeneous catalyst and base at relatively low temperatures (e.g., 310°C) at sub-critical pressures sufficient to maintain the liquid phase. Both precious metal and base metal catalysts were found to be active for the liquid phase hydrolysis and reforming of wood. Pt-based catalysts, particularly Pt-Re, were shown to be more selective toward breaking C-C bonds, resulting in a higher selectivity to hydrogen versus methane. Ni-based catalysts were found to prefer breaking C-O bonds, favoring the production of methane. The project showed that increasing the concentration of base (base to wood ratio) in the presence of Raney Ni catalysts resulted in greater selectivity toward hydrogen but at the expense of increasing the production of undesirable organic acids from the wood, lowering the amount of wood converted to gas. It was shown that by modifying Ni-based catalysts with dopants, it was possible to reduce the base concentration while maintaining the selectivity toward hydrogen and increasing wood conversion to gas versus organic acids. The final stage of the project was the construction and testing of a demonstration unit for H2 production. This continuous flow demonstration unit consisted of wood slurry and potassium carbonate feed pump systems, two reactors for hydrolysis and reforming, and a gas-liquid separation system. The technical challenges associated with unreacted wood fines and Raney Ni catalyst retention limited the demonstration unit to using a fixed bed Raney Ni catalyst form. The lower activity of the larger particle Raney Ni in turn limited the residence time and thus the wood mass flow feed rate to 50 g min-1 for a 1 wt% wood slurry. The project demonstrated continuous H2 yields with unmodified, fixed bed Raney Ni, from 63% to 100% with corresponding H2 selectivities of 6% to 21%, for periods of several hours. The fixed bed form of the Raney Ni exhibited signs of deactivation which requires further study.« less

  20. Comparative study on liquefaction of creosote and chromated copper arsenate (CCA)-treated wood and untreated southern pine wood: effects of acid catalyst content, liquefaction time, temperature, and phenol to wood ratio

    Treesearch

    Hui Pan; Chung-Yun Hse; Todd F. Shupe

    2009-01-01

    Creosote- and chromated copper arsenate (CCA)-treated wood waste and untreated southern pine wood were liquefied with phenol and sulfuric acid. The effects of sulfuric acid content, liquefaction time, liquefaction temperature, and phenol to wood ratio on liquefaction rate (i.e., wood residue content) were investigated and analyzed by analysis of variance (...

  1. Adhesives for Achieving Durable Bonds with Acetylated Wood

    Treesearch

    Charles Frihart; Rishawn Brandon; James Beecher; Rebecca Ibach

    2017-01-01

    Acetylation of wood imparts moisture durability, decay resistance, and dimensional stability to wood; however, making durable adhesive bonds with acetylated wood can be more difficult than with unmodified wood. The usual explanation is that the acetylated surface has fewer hydroxyl groups, resulting in a harder-to-wet surface and in fewer hydrogen bonds between wood...

  2. Finishes for Wood Decks

    Treesearch

    Mark Knaebe

    2013-01-01

    Wood decks have become an important part of residential construction. Wood decks can add versatile living space to a home and, with minimal maintenance, provide decades of use. However, wood decks are exposed to high levels of stress from severe weather conditions that shrink and swell the wood. Without proper maintenance, wood decks can develop problems such as checks...

  3. Surface characterization

    Treesearch

    Mandla A. Tshabalala

    2005-01-01

    Surface properties of wood play an important role when wood is used or processed into different commodities such as siding, joinery, textiles, paper, sorption media or wood composites. Thus, for example, the quality and durability of a wood coating are determined by the surface properties of the wood and the coating. The same is true for wood composites, as the...

  4. Effect of wood flour content on the optical color, surface chemistry, mechanical and morphological properties of wood flour/recycled high density polyethylene (rHDPE) composite

    NASA Astrophysics Data System (ADS)

    Sheng, Chan Kok; Amin, Khairul Anuar Mat; Kee, Kwa Bee; Hassan, Mohd Faiz; Ali, E. Ghapur E.

    2018-05-01

    In this study, effect of wood flour content on the color, surface chemistry, mechanical properties and surface morphology of wood-plastic composite (WPC) on different mixture ratios of recycled high density polyethylene (rHDPE) and wood flour were investigated in detail. The presence of wood flour in the composite indicates a significant total color change and a decrease of lightness. Functional groups of wood flour in WPC can be seen clearer from the Fourier transform infrared (FTIR) spectra as the wood flour content increases. The mechanical tensile testing shows that the tensile strength of Young's modulus is improved, whereas the strain and elongation at break were reduced by the addition of wood flour. The gap between the wood flour microvoid fibre and rHDPE matrix becomes closer when the wood flour content is increased as observed by scanning electron microscope (SEM) image. This finding implies a significant improvement on the interaction of interfacial adhesion between the rHDPE matrix and wood flour filler in the present WPC.

  5. Bacterial Community Succession in Pine-Wood Decomposition.

    PubMed

    Kielak, Anna M; Scheublin, Tanja R; Mendes, Lucas W; van Veen, Johannes A; Kuramae, Eiko E

    2016-01-01

    Though bacteria and fungi are common inhabitants of decaying wood, little is known about the relationship between bacterial and fungal community dynamics during natural wood decay. Based on previous studies involving inoculated wood blocks, strong fungal selection on bacteria abundance and community composition was expected to occur during natural wood decay. Here, we focused on bacterial and fungal community compositions in pine wood samples collected from dead trees in different stages of decomposition. We showed that bacterial communities undergo less drastic changes than fungal communities during wood decay. Furthermore, we found that bacterial community assembly was a stochastic process at initial stage of wood decay and became more deterministic in later stages, likely due to environmental factors. Moreover, composition of bacterial communities did not respond to the changes in the major fungal species present in the wood but rather to the stage of decay reflected by the wood density. We concluded that the shifts in the bacterial communities were a result of the changes in wood properties during decomposition and largely independent of the composition of the wood-decaying fungal communities.

  6. Bacterial Community Succession in Pine-Wood Decomposition

    PubMed Central

    Kielak, Anna M.; Scheublin, Tanja R.; Mendes, Lucas W.; van Veen, Johannes A.; Kuramae, Eiko E.

    2016-01-01

    Though bacteria and fungi are common inhabitants of decaying wood, little is known about the relationship between bacterial and fungal community dynamics during natural wood decay. Based on previous studies involving inoculated wood blocks, strong fungal selection on bacteria abundance and community composition was expected to occur during natural wood decay. Here, we focused on bacterial and fungal community compositions in pine wood samples collected from dead trees in different stages of decomposition. We showed that bacterial communities undergo less drastic changes than fungal communities during wood decay. Furthermore, we found that bacterial community assembly was a stochastic process at initial stage of wood decay and became more deterministic in later stages, likely due to environmental factors. Moreover, composition of bacterial communities did not respond to the changes in the major fungal species present in the wood but rather to the stage of decay reflected by the wood density. We concluded that the shifts in the bacterial communities were a result of the changes in wood properties during decomposition and largely independent of the composition of the wood-decaying fungal communities. PMID:26973611

  7. Trends...

    ERIC Educational Resources Information Center

    Creighton, B. W.; And Others

    1972-01-01

    Includes 11 sub-articles dealing with innovative recreational programs in Alaska, Michigan, Tennessee, Illinois, and California. Activities and facilities described include bus rides, artificial ice skating and skiing, rooftop playgrounds, wood structures, dam sites, and enclosed buildings. (NQ)

  8. Functional lignocellulosic materials prepared by ATRP from a wood scaffold.

    PubMed

    Cabane, Etienne; Keplinger, Tobias; Künniger, Tina; Merk, Vivian; Burgert, Ingo

    2016-08-10

    Wood, a natural and abundant source of organic polymers, has been used as a scaffold to develop novel wood-polymer hybrid materials. Through a two-step surface-initiated Atom Transfer Radical Polymerization (ATRP), the porous wood structure can be effectively modified with polymer chains of various nature. In the present study, polystyrene and poly(N-isopropylacrylamide) were used. As shown with various characterization techniques including confocal Raman microscopy, FTIR, and SEM/EDX, the native wood ultrastructure and features are retained and the polymer chains can be introduced deep within the wood, i.e. inside the wood cell walls. The physical properties of the new materials have been studied, and results indicate that the insertion of polymer chains inside the wood cell wall alters the intrinsic properties of wood to yield a hybrid composite material with new functionalities. This approach to the functionalization of wood could lead to the fabrication of a new class of interesting functional materials and promote innovative utilizations of the renewable resource wood.

  9. Functional lignocellulosic materials prepared by ATRP from a wood scaffold

    PubMed Central

    Cabane, Etienne; Keplinger, Tobias; Künniger, Tina; Merk, Vivian; Burgert, Ingo

    2016-01-01

    Wood, a natural and abundant source of organic polymers, has been used as a scaffold to develop novel wood-polymer hybrid materials. Through a two-step surface-initiated Atom Transfer Radical Polymerization (ATRP), the porous wood structure can be effectively modified with polymer chains of various nature. In the present study, polystyrene and poly(N-isopropylacrylamide) were used. As shown with various characterization techniques including confocal Raman microscopy, FTIR, and SEM/EDX, the native wood ultrastructure and features are retained and the polymer chains can be introduced deep within the wood, i.e. inside the wood cell walls. The physical properties of the new materials have been studied, and results indicate that the insertion of polymer chains inside the wood cell wall alters the intrinsic properties of wood to yield a hybrid composite material with new functionalities. This approach to the functionalization of wood could lead to the fabrication of a new class of interesting functional materials and promote innovative utilizations of the renewable resource wood. PMID:27506369

  10. Comparing the VOC emissions between air-dried and heat-treated Scots pine wood

    NASA Astrophysics Data System (ADS)

    Manninen, Anne-Marja; Pasanen, Pertti; Holopainen, Jarmo K.

    The emissions of volatile organic compounds (VOCs) from air-dried Scots pine wood and from heat-treated Scots pine wood were compared with GC-MS analysis. Air-dried wood blocks released about 8 times more total VOCs than heat-treated (24 h at 230°C) ones. Terpenes were clearly the main compound group in the air-dried wood samples, whereas aldehydes and carboxylic acids and their esters dominated in the heat-treated wood samples. Only 14 compounds out of 41 identified individual compounds were found in both wood samples indicating considerable changes in VOC emission profile during heat-treatment process. Of individual compounds α-pinene, 3-carene and hexanal were the most abundant ones in the air-dried wood. By contrast, in the heat-treated wood 2-furancarboxaldehyde, acetic acid and 2-propanone were the major compounds of VOC emission. Current emission results reveal that significant chemical changes have occurred, and volatile monoterpenes and other low-molecular-weight compounds have evaporated from the wood during the heat-treatment process when compared to air-dried wood. Major chemical changes detected in VOC emissions are explained by the thermal degradation and oxidation of main constituents in wood. The results suggest that if heat-treated wood is used in interior carpentry, emissions of monoterpenes are reduced compared to air-dried wood, but some irritating compounds might be released into indoor air.

  11. Wood adhesives : vital for producing most wood products

    Treesearch

    Charles R. Frihart

    2011-01-01

    A main route for the efficient utilization of wood resources is to reduce wood to small pieces and then bond them together (Frihart and Hunt 2010). Although humankind has been bonding wood since early Egyptian civilizations, the quality and quantity of bonded wood products has increased dramatically over the past 100 years with the development of new adhesives and...

  12. Chapter 8:Surface Characterization

    Treesearch

    Mandla A. Tshabalala; Joseph Jakes; Mark R. VanLandingham; Shaoxia Wang; Jouko Peltonen

    2013-01-01

    Surface properties of wood play an important role when wood is used or processed into different commodities such as siding, joinery, textiles, paper, sorption media, or wood composites. Thus, for example, the quality and durability of a wood coating are determined by the surface properties of the wood and the coating. The same is true for wood composites where the...

  13. 77 FR 69454 - Change in Bank Control Notices; Acquisitions of Shares of a Bank or Bank Holding Company

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-19

    ..., South Carolina. B. Federal Reserve Bank of Chicago (Colette A. Fried, Assistant Vice President) 230.... Woods, Bluffton, South Carolina, individually and as part of a group acting in concert with Edgar Woods.... Woods, Milton Woods Jr. and Susan H Woods, both of Ridgeland, South Carolina, and J. Eric Woods...

  14. Chapter 02: Basic wood biology—Anatomy for identification

    Treesearch

    Alex Wiedenhoeft

    2011-01-01

    Before the topics of using a hand lens, preparing wood for observation, and understanding the characters used in wood identification can be tackled, a general introduction to the biology of wood must be undertaken. The woods in commercial trade in Central America come almost exclusively from trees, so the discussion of wood biology is restricted to trees here, though...

  15. Uses and desirable properties of wood in the 21st century

    Treesearch

    Theodore Wegner; Kenneth E. Skog; Peter J. Ince; Charles J. Michler

    2010-01-01

    The desirability of specific wood properties is driven by a number of social, economic, and environmental factors that influence wood-use trends. This article discusses current continuing commercial uses of wood, significant new or emerging commercial uses, and desirable wood properties indicated by projected changes in wood use. Emerging issues and applications such...

  16. Interaction of copper wood preservatives and adhesives

    Treesearch

    Charles R. Frihart

    2003-01-01

    Compared to other substrates, wood is generally easy to bond. However, adhesion is diminished when the wood surface is covered by chemicals, whether natural oils and resins or added chemicals. Among the chemicals added to wood are fire retardants and wood preservatives. Chromated copper arsenate (CCA) has been widely used to protect wood against rot and termites, but...

  17. Characterization of Juvenile wood in Lodgepole Pine in the Intermountain West

    Treesearch

    Thomas M. Gorman; David E. Kretschmann

    2012-01-01

    Juvenile wood (core wood) is typically characterized as being less dimensionally stable and having lower mechanical properties than mature wood. Determination of the age of transition from juvenile wood to mature wood can provide basic information needed to assess dimensional stability and better utilize small-diameter trees growing in the intermountain west as solid-...

  18. Many Roles of Wood Adhesives

    Treesearch

    Charles R. Frihart

    2014-01-01

    Although wood bonding is one of the oldest applications of adhesives, going back to early recorded history (1), some aspects of wood bonds are still not fully understood. Most books in the general area of adhesives and adhesion do not cover wood bonding. However, a clearer understanding of wood bonding and wood adhesives can lead to improved products. This is important...

  19. Thermal decomposition of wood: influence of wood components and cellulose crystallite size.

    PubMed

    Poletto, Matheus; Zattera, Ademir J; Forte, Maria M C; Santana, Ruth M C

    2012-04-01

    The influence of wood components and cellulose crystallinity on the thermal degradation behavior of different wood species has been investigated using thermogravimetry, chemical analysis and X-ray diffraction. Four wood samples, Pinus elliottii (PIE), Eucalyptus grandis (EUG), Mezilaurus itauba (ITA) and Dipteryx odorata (DIP) were used in this study. The results showed that higher extractives contents associated with lower crystallinity and lower cellulose crystallite size can accelerate the degradation process and reduce the wood thermal stability. On the other hand, the thermal decomposition of wood shifted to higher temperatures with increasing wood cellulose crystallinity and crystallite size. These results indicated that the cellulose crystallite size affects the thermal degradation temperature of wood species. Copyright © 2012. Published by Elsevier Ltd.

  20. 78 FR 34105 - Proposed Information Collection Activity; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-06

    ... include Natural Gas, Electricity, Fuel Oil, Propane, Wood and Coal. The average annual primary home energy... using a secondary source of heat. Annual Heating Fuel Consumption: The grantee would need to collect...

  1. Remediation System Evaluation, MacGillis and Gibbs Superfund Site

    EPA Pesticide Factsheets

    The site was a wood preserving facility that is no longer active. Key contaminants at the site includepentachlorophenol (PCP), chromium, and to a much lesser extent dioxin, arsenic, and polynucleararomatic hydrocarbons (PAHs).

  2. Quantification of bioactive compounds in pulps and by-products of tropical fruits from Brazil.

    PubMed

    Ribeiro da Silva, Larissa Morais; Teixeira de Figueiredo, Evania Altina; Silva Ricardo, Nagila Maria Pontes; Pinto Vieira, Icaro Gusmao; Wilane de Figueiredo, Raimundo; Brasil, Isabella Montenegro; Gomes, Carmen L

    2014-01-15

    This study aimed to quantify the levels of resveratrol, coumarin, and other bioactives in pulps and by-products of twelve tropical fruits from Brazil obtained during pulp production process. Pineapple, acerola, monbin, cashew apple, guava, soursop, papaya, mango, passion fruit, surinam cherry, sapodilla, and tamarind pulps were evaluated as well as their by-products (peel, pulp's leftovers, and seed). Total phenolic, anthocyanins, yellow flavonoids, β-carotene and lycopene levels were also determined. Resveratrol was identified in guava and surinam cherry by-products and coumarin in passion fruit, guava and surinam cherry by-products and mango pulp. These fruit pulp and by-products could be considered a new natural source of both compounds. Overall, fruit by-products presented higher (P<0.05) bioactive content than their respective fruit pulps. This study provides novel information about tropical fruits and their by-products bioactive composition, which is essential for the understanding of their nutraceutical potential and future application in the food industry. Published by Elsevier Ltd.

  3. Statistical optimization of process parameters for the production of tannase by Aspergillus flavus under submerged fermentation.

    PubMed

    Mohan, S K; Viruthagiri, T; Arunkumar, C

    2014-04-01

    Production of tannase by Aspergillus flavus (MTCC 3783) using tamarind seed powder as substrate was studied in submerged fermentation. Plackett-Burman design was applied for the screening of 12 medium nutrients. From the results, the significant nutrients were identified as tannic acid, magnesium sulfate, ferrous sulfate and ammonium sulfate. Further the optimization of process parameters was carried out using response surface methodology (RSM). RSM has been applied for designing of experiments to evaluate the interactive effects through a full 31 factorial design. The optimum conditions were tannic acid concentration, 3.22 %; fermentation period, 96 h; temperature, 35.1 °C; and pH 5.4. Higher value of the regression coefficient (R 2  = 0.9638) indicates excellent evaluation of experimental data by second-order polynomial regression model. The RSM revealed that a maximum tannase production of 139.3 U/ml was obtained at the optimum conditions.

  4. The raft of the Saint-Jean River, Gaspé (Québec, Canada): A dynamic feature trapping most of the wood transported from the catchment

    NASA Astrophysics Data System (ADS)

    Boivin, Maxime; Buffin-Bélanger, Thomas; Piégay, Hervé

    2015-02-01

    The rivers of the Gaspé Peninsula, Québec (Canada), a coastal drainage system of the St. Lawrence River, receive and transport vast quantities of large wood. The rapid rate of channel shifting caused by high-energy flows and noncohesive banks allows wood recruitment that in turn greatly influences river dynamics. The delta of the Saint-Jean River has accumulated wood since 1960, leading to frequent avulsions over that time period. The wood raft there is now more than 3-km in length, which is unusual but natural. This jam configuration allows a unique opportunity to estimate a wood budget at the scale of a long river corridor and to better understand the dynamics of large wood (LW) in rivers. A wood budget includes the evaluation of wood volumes (i) produced by bank erosion (input), (ii) still in transit in the river corridor (deposited on sand bars or channel edges), and (iii) accumulated in the delta (output). The budget is based on an analysis of aerial photos dating back to 1963 as well as surveys carried out in 2010, all of which were used to locate and describe large wood accumulations along a 60-km river section. The main results of this paper show that the raft formation in the delta is dynamic and can be massive, but it is a natural process. Considering the estimated wood volume trapped in the delta from 1963 to 2013 (≈ 25,000 m3), two important points are revealed by the quantification of the wood recruitment volume from 1963 to 2004 (≈ 27,000 m3 ± 400 m3) and of the wood volume stored on the bars in 2010 (≈ 5950 m3). First, the recruitment of large wood from lateral migration for the 40-year period can account for the volume of large wood in the delta and in transit. Second, the excess wood volume produced by lateral migration and avulsion represents a minimum estimation of the large wood trapped on the floodplain owing to wood volume that has decomposed and large wood that exited the river system. Rafts are major trapping structures that provide good potential sites to monitor wood delivery from the catchment through time and allow estimations of LW residence time while in transit. These results contribute to understanding the interannual large wood dynamics in the Saint-Jean River and can assist river managers in determining sustainable solutions for coping with the issue of wood rafts in rivers.

  5. Genetic and hormonal regulation of cambial development.

    PubMed

    Ursache, Robertas; Nieminen, Kaisa; Helariutta, Ykä

    2013-01-01

    The stems and roots of most dicot plants increase in diameter by radial growth, due to the activity of secondary meristems. Two types of meristems function in secondary plant body formation: the vascular cambium, which gives rise to secondary xylem and phloem, and the cork cambium, which produces a bark layer that replaces the epidermis and protects the plant stem from mechanical damage and pathogens. Cambial development, the initiation and activity of the vascular cambium, leads to an accumulation of wood, the secondary xylem tissue. The thick, cellulose-rich cell walls of wood provide a source of cellulose and have the potential to be used as a raw material for sustainable and renewable energy production. In this review, we will discuss what is known about the mechanisms regulating the cambium and secondary tissue development. Copyright © Physiologia Plantarum 2012.

  6. Effects of copper amine treatments on mechanical, biological and surface/interphase properties of poly (vinyl chloride)/wood composites

    NASA Astrophysics Data System (ADS)

    Jiang, Haihong

    2005-11-01

    The copper ethanolamine (CuEA) complex was used as a wood surface modifier and a coupling agent for wood-PVC composites. Mechanical properties of composites, such as unnotched impact strength, flexural strength and flexural toughness, were significantly increased, and fungal decay weight loss was dramatically decreased by wood surface copper amine treatments. It is evident that copper amine was a very effective coupling agent and decay inhibitor for PVC/wood flour composites, especially in high wood flour loading level. A DSC study showed that the heat capacity differences (DeltaCp) of composites before and after PVC glass transition were reduced by adding wood particles. A DMA study revealed that the movements of PVC chain segments during glass transition were limited and obstructed by the presence of wood molecule chains. This restriction effect became stronger by increasing wood flour content and by using Cu-treated wood flour. Wood flour particles acted as "physical cross-linking points" inside the PVC matrix, resulting in the absence of the rubbery plateau of PVC and higher E', E'' above Tg, and smaller tan delta peaks. Enhanced mechanical performances were attributed to the improved wetting condition between PVC melts and wood surfaces, and the formation of a stronger interphase strengthened by chemical interactions between Cu-treated wood flour and the PVC matrix. Contact angles of PVC solution drops on Cu-treated wood surfaces were decreased dramatically compared to those on the untreated surfaces. Acid-base (polar), gammaAB, electron-acceptor (acid) (gamma +), electron-donor (base) (gamma-) surface energy components and the total surface energies increased after wood surface Cu-treatments, indicating a strong tendency toward acid-base or polar interactions. Improved interphase and interfacial adhesion were further confirmed by measuring interfacial shear strength between wood and the PVC matrix.

  7. The trait contribution to wood decomposition rates of 15 Neotropical tree species.

    PubMed

    van Geffen, Koert G; Poorter, Lourens; Sass-Klaassen, Ute; van Logtestijn, Richard S P; Cornelissen, Johannes H C

    2010-12-01

    The decomposition of dead wood is a critical uncertainty in models of the global carbon cycle. Despite this, relatively few studies have focused on dead wood decomposition, with a strong bias to higher latitudes. Especially the effect of interspecific variation in species traits on differences in wood decomposition rates remains unknown. In order to fill these gaps, we applied a novel method to study long-term wood decomposition of 15 tree species in a Bolivian semi-evergreen tropical moist forest. We hypothesized that interspecific differences in species traits are important drivers of variation in wood decomposition rates. Wood decomposition rates (fractional mass loss) varied between 0.01 and 0.31 yr(-1). We measured 10 different chemical, anatomical, and morphological traits for all species. The species' average traits were useful predictors of wood decomposition rates, particularly the average diameter (dbh) of the tree species (R2 = 0.41). Lignin concentration further increased the proportion of explained inter-specific variation in wood decomposition (both negative relations, cumulative R2 = 0.55), although it did not significantly explain variation in wood decomposition rates if considered alone. When dbh values of the actual dead trees sampled for decomposition rate determination were used as a predictor variable, the final model (including dead tree dbh and lignin concentration) explained even more variation in wood decomposition rates (R2 = 0.71), underlining the importance of dbh in wood decomposition. Other traits, including wood density, wood anatomical traits, macronutrient concentrations, and the amount of phenolic extractives could not significantly explain the variation in wood decomposition rates. The surprising results of this multi-species study, in which for the first time a large set of traits is explicitly linked to wood decomposition rates, merits further testing in other forest ecosystems.

  8. Changing Face of Wood Science in Modern Era: Contribution of Nanotechnology.

    PubMed

    Mishra, Pawan Kumar; Giagli, Kyriaki; Tsalagkas, Dimitrios; Mishra, Harshita; Talegaonkar, Sushma; Gryc, Vladimír; Wimmer, Rupert

    2018-02-14

    Wood science and nanomaterials science interact together in two different aspects; a) fabrication of lignocellulosic nanomaterials derived from wood and plant-based sources and b) surface or bulk wood modification by nanoparticles. In this review, we attempt to visualize the impact of nanoparticles on the wood coating and preservation treatments based on a thorough registration of the patent databases. The study was carried out as an overview of the scientifically most followed trends on nanoparticles utilization in wood science and wood protection depicted by recent universal filed patents. This review is exclusively targeted on the solid (timber) wood as a subject material. Utilization of mainly metal nanoparticles as photoprotection, antibacterial, antifungal, antiabrasive and functional component on wood modification treatments was found to be widely patented. Additionally, an apparent minimization in the emission of volatile organic compounds (VOCs) has been succeeded. Bulk wood preservation and more importantly, wood coating, splay the range of strengthening wood dimensional stability and biological degradation, against moisture absorption and fungi respectively. Nanoparticle materials have addressed various issues of wood science in a more efficient and environmental way than the traditional methods. Nevertheless, abundant tests and regulations are still needed before industrializing or recycling these products. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  9. DNA Damage among Wood Workers Assessed with the Comet Assay

    PubMed Central

    Bruschweiler, Evin Danisman; Wild, Pascal; Huynh, Cong Khanh; Savova-Bianchi, Dessislava; Danuser, Brigitta; Hopf, Nancy B.

    2016-01-01

    Exposure to wood dust, a human carcinogen, is common in wood-related industries, and millions of workers are occupationally exposed to wood dust worldwide. The comet assay is a rapid, simple, and sensitive method for determining DNA damage. The objective of this study was to investigate the DNA damage associated with occupational exposure to wood dust using the comet assay (peripheral blood samples) among nonsmoking wood workers (n = 31, furniture and construction workers) and controls (n = 19). DNA damage was greater in the group exposed to composite wood products compared to the group exposed to natural woods and controls (P < 0.001). No difference in DNA damage was observed between workers exposed to natural woods and controls (P = 0.13). Duration of exposure and current dust concentrations had no effect on DNA damage. In future studies, workers’ exposures should include cumulative dust concentrations and exposures originating from the binders used in composite wood products. PMID:27398027

  10. Characterization of a gel in the cell wall to elucidate the paradoxical shrinkage of tension wood.

    PubMed

    Clair, Bruno; Gril, Joseph; Di Renzo, Francesco; Yamamoto, Hiroyuki; Quignard, Françoise

    2008-02-01

    Wood behavior is characterized by high sensibility to humidity and strongly anisotropic properties. The drying shrinkage along the fibers, usually small due to the reinforcing action of cellulosic microfibrils, is surprisingly high in the so-called tension wood, produced by trees to respond to strong reorientation requirements. In this study, nitrogen adsorption-desorption isotherms of supercritically dried tension wood and normal wood show that the tension wood cell wall has a gel-like structure characterized by a pore surface more than 30 times higher than that in normal wood. Syneresis of the tension wood gel explains its paradoxical drying shrinkage. This result could help to reduce technological problems during drying. Potential applications in biomechanics and biomimetics are worth investigating, considering that, in living trees, tension wood produces tensile growth stresses 10 times higher than that of normal wood.

  11. Heavy Metal-Free Tannin from Bark for Sustainable Energy Storage

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mukhopadhyay, Alolika; Jiao, Yucong; Katahira, Rui

    A novel renewable cathode made from earth abundant, low-cost materials can contribute to the intermittent storage needs of renewable energy-based society. In this work, we report for the first-time tannin from Nature as a cathode material. Our approach exploits the charge storage mechanism of the redox active quinone moiety. Tannins extracted from tree bark using environmental friendly aqueous solvents have the highest phenol content (5.56 mol g -1) among all the natural phenolic biopolymers, 5000 times higher than lignin. Tannins coupled with a conductive polymer polypyrrole acquire high specific capacitance values of 370 F g -1 at 0.5 A gmore » -1 as well as excellent rate performance of 196 F g -1 at 25 A g -1. Additionally, we employed carbonized wood as an electrode substrate to produce a sustainable electrochemical device with dramatically improved performance compared to conventional devices. The high surface area provided by the well-aligned, cellular porosity of wood-derived substrate combined with the high mobility of ions and electrons in the carbonized cell walls and deposited tannin can achieve an areal capacitance of 4.6 F cm -2 at 1 mA cm -2, which is 1.5 times higher than activated wood carbon.« less

  12. Heavy Metal-Free Tannin from Bark for Sustainable Energy Storage

    DOE PAGES

    Mukhopadhyay, Alolika; Jiao, Yucong; Katahira, Rui; ...

    2017-11-30

    A novel renewable cathode made from earth abundant, low-cost materials can contribute to the intermittent storage needs of renewable energy-based society. In this work, we report for the first-time tannin from Nature as a cathode material. Our approach exploits the charge storage mechanism of the redox active quinone moiety. Tannins extracted from tree bark using environmental friendly aqueous solvents have the highest phenol content (5.56 mol g -1) among all the natural phenolic biopolymers, 5000 times higher than lignin. Tannins coupled with a conductive polymer polypyrrole acquire high specific capacitance values of 370 F g -1 at 0.5 A gmore » -1 as well as excellent rate performance of 196 F g -1 at 25 A g -1. Additionally, we employed carbonized wood as an electrode substrate to produce a sustainable electrochemical device with dramatically improved performance compared to conventional devices. The high surface area provided by the well-aligned, cellular porosity of wood-derived substrate combined with the high mobility of ions and electrons in the carbonized cell walls and deposited tannin can achieve an areal capacitance of 4.6 F cm -2 at 1 mA cm -2, which is 1.5 times higher than activated wood carbon.« less

  13. Metabolomic and high-throughput sequencing analysis-modern approach for the assessment of biodeterioration of materials from historic buildings.

    PubMed

    Gutarowska, Beata; Celikkol-Aydin, Sukriye; Bonifay, Vincent; Otlewska, Anna; Aydin, Egemen; Oldham, Athenia L; Brauer, Jonathan I; Duncan, Kathleen E; Adamiak, Justyna; Sunner, Jan A; Beech, Iwona B

    2015-01-01

    Preservation of cultural heritage is of paramount importance worldwide. Microbial colonization of construction materials, such as wood, brick, mortar, and stone in historic buildings can lead to severe deterioration. The aim of the present study was to give modern insight into the phylogenetic diversity and activated metabolic pathways of microbial communities colonized historic objects located in the former Auschwitz II-Birkenau concentration and extermination camp in Oświecim, Poland. For this purpose we combined molecular, microscopic and chemical methods. Selected specimens were examined using Field Emission Scanning Electron Microscopy (FESEM), metabolomic analysis and high-throughput Illumina sequencing. FESEM imaging revealed the presence of complex microbial communities comprising diatoms, fungi and bacteria, mainly cyanobacteria and actinobacteria, on sample surfaces. Microbial diversity of brick specimens appeared higher than that of the wood and was dominated by algae and cyanobacteria, while wood was mainly colonized by fungi. DNA sequences documented the presence of 15 bacterial phyla representing 99 genera including Halomonas, Halorhodospira, Salinisphaera, Salinibacterium, Rubrobacter, Streptomyces, Arthrobacter and nine fungal classes represented by 113 genera including Cladosporium, Acremonium, Alternaria, Engyodontium, Penicillium, Rhizopus, and Aureobasidium. Most of the identified sequences were characteristic of organisms implicated in deterioration of wood and brick. Metabolomic data indicated the activation of numerous metabolic pathways, including those regulating the production of primary and secondary metabolites, for example, metabolites associated with the production of antibiotics, organic acids and deterioration of organic compounds. The study demonstrated that a combination of electron microscopy imaging with metabolomic and genomic techniques allows to link the phylogenetic information and metabolic profiles of microbial communities and to shed new light on biodeterioration processes.

  14. Metabolomic and high-throughput sequencing analysis—modern approach for the assessment of biodeterioration of materials from historic buildings

    PubMed Central

    Gutarowska, Beata; Celikkol-Aydin, Sukriye; Bonifay, Vincent; Otlewska, Anna; Aydin, Egemen; Oldham, Athenia L.; Brauer, Jonathan I.; Duncan, Kathleen E.; Adamiak, Justyna; Sunner, Jan A.; Beech, Iwona B.

    2015-01-01

    Preservation of cultural heritage is of paramount importance worldwide. Microbial colonization of construction materials, such as wood, brick, mortar, and stone in historic buildings can lead to severe deterioration. The aim of the present study was to give modern insight into the phylogenetic diversity and activated metabolic pathways of microbial communities colonized historic objects located in the former Auschwitz II–Birkenau concentration and extermination camp in Oświecim, Poland. For this purpose we combined molecular, microscopic and chemical methods. Selected specimens were examined using Field Emission Scanning Electron Microscopy (FESEM), metabolomic analysis and high-throughput Illumina sequencing. FESEM imaging revealed the presence of complex microbial communities comprising diatoms, fungi and bacteria, mainly cyanobacteria and actinobacteria, on sample surfaces. Microbial diversity of brick specimens appeared higher than that of the wood and was dominated by algae and cyanobacteria, while wood was mainly colonized by fungi. DNA sequences documented the presence of 15 bacterial phyla representing 99 genera including Halomonas, Halorhodospira, Salinisphaera, Salinibacterium, Rubrobacter, Streptomyces, Arthrobacter and nine fungal classes represented by 113 genera including Cladosporium, Acremonium, Alternaria, Engyodontium, Penicillium, Rhizopus, and Aureobasidium. Most of the identified sequences were characteristic of organisms implicated in deterioration of wood and brick. Metabolomic data indicated the activation of numerous metabolic pathways, including those regulating the production of primary and secondary metabolites, for example, metabolites associated with the production of antibiotics, organic acids and deterioration of organic compounds. The study demonstrated that a combination of electron microscopy imaging with metabolomic and genomic techniques allows to link the phylogenetic information and metabolic profiles of microbial communities and to shed new light on biodeterioration processes. PMID:26483760

  15. Prevalence and duration of social security benefits allowed to workers with asthma in Brazil in 2008.

    PubMed

    Branco, Anadergh Barbosa de Abreu; Ildefonso, Simone de Andrade Goulart

    2012-01-01

    To determine the prevalence and duration of social security benefits (SSBs) claims to registered workers with asthma in Brazil by the Brazilian National Institute of Social Security in 2008. This was a retrospective, descriptive study, based on information obtained from the Brazilian Unified Benefit System database, on the number of SSB claims granted to registered workers with asthma in 2008. The reference population was the monthly mean number of workers registered in the Brazilian Social Registry Database in 2008. The variables studied were type of economic activity, gender, age, and type/duration of the SSB claim. The relationship between work and asthma was evaluated by the prevalence ratio (PR) between work-related and non-work-related SSB claims for asthma. In 2008, 2,483 SSB claims were granted for asthma, with a prevalence of 7.5 allowances per 100,000 registered workers. The prevalence was higher among females than among males (PR = 2.1 between the sexes). Workers > 40 years of age were 2.5 times more likely to be granted an SSB claim for asthma than were younger workers. The prevalence was highest among workers engaged in the following types of economic activity: sewage, wood and wood product manufacturing, and furniture manufacturing (78.8, 22.4, and 22.2 claims/100,000 registered workers, respectively). The median (interquartile range) duration of SSB claims for asthma was 49 (28-87) days. Asthma is a major cause of sick leave, and its etiology has a strong occupational component. This has a major impact on employers, employees, and the social security system. Being female, being > 40 years of age, and working in the areas of urban sanitation/sewage, wood and wood product manufacturing, and furniture manufacturing increase the chance of sick leave due to asthma.

  16. The effects of wood dusts on the redox status and cell death in mouse macrophages (RAW 264.7) and human leukocytes in vitro.

    PubMed

    Naarala, J; Kasanen, J-P; Pasanen, P; Pasanen, A-L; Liimatainen, A; Pennanen, S; Liesivuori, J

    2003-07-11

    Wood dusts are classified as carcinogenic to humans and also produce other toxic, allergic, and acute effects in woodworkers. However, little is known about causative agents in wood dusts and their mechanisms of action. The effects of different tree species and particle size for biological activity were studied. The differences in the production of reactive oxygen species (ROS) and cell death (necrotic and apoptotic) between mouse macrophage (RAW 264.7) cells and human polymorphonuclear leukocytes (PMNL) for pine, birch, and beech dust exposures were investigated in vitro. The pine and birch dust exposure (1-100 microg/ml) produced concentration-dependent ROS production in both the cells, which was one order of magnitude higher with pine dust. The ROS production was faster in human PNML than murine RAW cells. The higher concentrations (500 and/or 1000 microg/ml) decreased ROS formation. With pine and birch dust exposure, this was probably due to the necrotic cell death. The pine dust concentrations of 500 and 1000 microg/ml were cytotoxic to human PMNL. The beech dust exposure activated the ROS production and decreased the cell viability only at the highest concentrations, being least potent of the three dusts. A sign of the apoptotic cell death in the murine RAW cells was observed at the pine dust concentration of 100 microg/ml. The exposure to the birch and beech dusts with a smaller particle size (<5 microm) produced greater ROS production than exposure to the corresponding dust with a wide range of particle sizes. However, changing the particle size did not affect the cell viability. The results indicate that the type of wood dust (tree species and possibly particle size) has a significant impact on the function and viability of phagocytic cells.

  17. Does simulated acid rain increase the leaching of cadmium from wood ash to toxic levels to coniferous forest humus microbes?

    PubMed

    Perkiömäki, Jonna; Fritze, Hannu

    2003-05-01

    Abstract Wood ash contains Cd in concentrations not permitted for fertilization use in agriculture (>3 mg kg(-1)). It has been shown that spiking ash with Cd to concentrations of 1000 mg kg(-1) induced no further changes in humus microbial activity and community structure as ash alone. To accelerate the weathering process and thus to liberate the spiked Cd from the ash, three treatments - wood ash (A), Cd spiked wood ash (ACd, 1000 mg Cd kg(-1) ash), both applied at a fertilization rate of 5000 kg ha(-1), together with a control (C) - were performed in microcosms and incubated in field condition under two types of irrigation - water and simulated acid rain. During the incubation period of one growing season the simulated acid rain plots received a sulfur load of 3.64 g S m(-2), which was 15 times more than the S deposition on the water irrigated plots. The treatments resulted in a mean Cd increase of the humus from 0.23 mg kg(-1) of the C treatment to 0.52 and 39.5 mg kg(-1) of the A and ACd treatments, respectively. The irrigation had no further effect on the result. The microbial activity, measured as soil basal respiration, and the microbial community structure, measured as humus phospholipid fatty acid and 16S and 18S polymerase chain reaction/denaturing gradient gel electrophoresis patterns, changed only due to the ash (A and ACd treatments) fertilization irrespective of the irrigation. The bacterial biosensor, emitting light in the presence of bioavailable Cd, did not react to any of the treatments. This result shows that Cd in ash was not leached into the humus due to increased deposition of acidified rain.

  18. Modeling hormonal control of cambium proliferation.

    PubMed

    Oles, Vladyslav; Panchenko, Alexander; Smertenko, Andrei

    2017-01-01

    Rise of atmospheric CO2 is one of the main causes of global warming. Catastrophic climate change can be avoided by reducing emissions and increasing sequestration of CO2. Trees are known to sequester CO2 during photosynthesis, and then store it as wood biomass. Thus, breeding of trees with higher wood yield would mitigate global warming as well as augment production of renewable construction materials, energy, and industrial feedstock. Wood is made of cellulose-rich xylem cells produced through proliferation of a specialized stem cell niche called cambium. Importance of cambium in xylem cells production makes it an ideal target for the tree breeding programs; however our knowledge about control of cambium proliferation remains limited. The morphology and regulation of cambium are different from those of stem cell niches that control axial growth. For this reason, translating the knowledge about axial growth to radial growth has limited use. Furthermore, genetic approaches cannot be easily applied because overlaying tissues conceal cambium from direct observation and complicate identification of mutants. To overcome the paucity of experimental tools in cambium biology, we constructed a Boolean network CARENET (CAmbium REgulation gene NETwork) for modelling cambium activity, which includes the key transcription factors WOX4 and HD-ZIP III as well as their potential regulators. Our simulations predict that: (1) auxin, cytokinin, gibberellin, and brassinosteroids act cooperatively in promoting transcription of WOX4 and HD-ZIP III; (2) auxin and cytokinin pathways negatively regulate each other; (3) hormonal pathways act redundantly in sustaining cambium activity; (4) individual cambium cells can have diverse molecular identities. CARENET can be extended to include components of other signalling pathways and be integrated with models of xylem and phloem differentiation. Such extended models would facilitate breeding trees with higher wood yield.

  19. Large wood transport and jam formation in a series of flume experiments

    NASA Astrophysics Data System (ADS)

    Davidson, S. L.; MacKenzie, L. G.; Eaton, B. C.

    2015-12-01

    Large wood has historically been removed from streams, resulting in the depletion of in-stream wood in waterways worldwide. As wood increases morphological and hydraulic complexity, the addition of large wood is commonly employed as a means to rehabilitate in-stream habitat. At present, however, the scientific understanding of wood mobilization and transport is incomplete. This paper presents results from a series of four flume experiments in which wood was added to a reach to investigate the piece and reach characteristics that determine wood stability and transport, as well as the time scale required for newly recruited wood to self-organize into stable jams. Our results show that wood transitions from a randomly distributed newly recruited state to a self-organized, or jam-stabilized state, over the course of a single bankfull flow event. Statistical analyses of piece mobility during this transitional period indicate that piece irregularities, especially rootwads, dictate the stability of individual wood pieces; rootwad presence or absence accounts for up to 80% of the variance explained by linear regression models for transport distance. Furthermore, small pieces containing rootwads are especially stable. Large ramped pieces provide nuclei for the formation of persistent wood jams, and the frequency of these pieces in the reach impacts the travel distance of mobile wood. This research shows that the simulation of realistic wood dynamics is possible using a simplified physical model, and also has management implications, as it suggests that randomly added wood may organize into persistent, stable jams, and characterizes the time scale for this transition.

  20. Furniture wood wastes: Experimental property characterisation and burning tests

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Tatano, Fabio; Barbadoro, Luca; Mangani, Giovanna

    2009-10-15

    Referring to the industrial wood waste category (as dominant in the provincial district of Pesaro-Urbino, Marche Region, Italy), this paper deals with the experimental characterisation and the carrying out of non-controlled burning tests (at lab- and pilot-scale) for selected 'raw' and primarily 'engineered' ('composite') wood wastes. The property characterisation has primarily revealed the following aspects: potential influence on moisture content of local weather conditions at outdoor wood waste storage sites; generally, higher ash contents in 'engineered' wood wastes as compared with 'raw' wood wastes; and relatively high energy content values of 'engineered' wood wastes (ranging on the whole from 3675more » to 5105 kcal kg{sup -1} for HHV, and from 3304 to 4634 kcal kg{sup -1} for LHV). The smoke qualitative analysis of non-controlled lab-scale burning tests has primarily revealed: the presence of specific organic compounds indicative of incomplete wood combustion; the presence exclusively in 'engineered' wood burning tests of pyrroles and amines, as well as the additional presence (as compared with 'raw' wood burning) of further phenolic and containing nitrogen compounds; and the potential environmental impact of incomplete industrial wood burning on the photochemical smog phenomenon. Finally, non-controlled pilot-scale burning tests have primarily given the following findings: emission presence of carbon monoxide indicative of incomplete wood combustion; higher nitrogen oxide emission values detected in 'engineered' wood burning tests as compared with 'raw' wood burning test; and considerable generation of the respirable PM{sub 1} fraction during incomplete industrial wood burning.« less

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