ESO 2.2-m WFI Image of the Tarantula Nebula

NASA Image and Video Library

2017-12-08

NASA image release May 11, 2010 Hubble Catches Heavyweight Runaway Star Speeding from 30 Doradus Image: ESO 2.2-m WFI Image of the Tarantula Nebula A blue-hot star, 90 times more massive than our Sun, is hurtling across space fast enough to make a round trip from Earth to the Moon in merely two hours. Though the speed is not a record-breaker, it is unique to find a homeless star that has traveled so far from its nest. The only way the star could have been ejected from the star cluster where it was born is through a tussle with a rogue star that entered the binary system where the star lived, which ejected the star through a dynamical game of stellar pinball. This is strong circumstantial evidence for stars as massive as 150 times our Sun's mass living in the cluster. Only a very massive star would have the gravitational energy to eject something weighing 90 solar masses. The runaway star is on the outskirts of the 30 Doradus nebula, a raucous stellar breeding ground in the nearby Large Magellanic Cloud. The finding bolsters evidence that the most massive stars in the local universe reside in 30 Doradus, making it a unique laboratory for studying heavyweight stars. 30 Doradus, also called the Tarantula Nebula, is roughly 170,000 light-years from Earth. To learn more about this image go to: www.nasa.gov/mission_pages/hubble/science/runaway-star.html Credit: NASA/ESO, J. Alves (Calar Alto, Spain), and B. Vandame and Y. Beletski (ESO) NASA Goddard Space Flight Center is home to the nation's largest organization of combined scientists, engineers and technologists that build spacecraft, instruments and new technology to study the Earth, the sun, our solar system, and the universe.

Cylindrical Bragg mirrors on leg segments of the male Bolivian blueleg tarantula Pamphobeteus antinous (Theraphosidae).

PubMed

Simonis, Priscilla; Bay, Annick; Welch, Victoria L; Colomer, Jean-François; Vigneron, Jean Pol

2013-03-25

The large male tarantula Pamphobeteus antinous is easily recognized at the presence of blue-violet iridescent bristles on some of the segments of its legs and pedipalps. The optical properties of these colored appendages have been measured and the internal geometrical structure of the bristles have been investigated. The coloration is shown to be caused by a curved coaxial multilayer which acts as a "cylindrical Bragg mirror".

VizieR Online Data Catalog: DIB in VLT-FLAMES Tarantula Survey (van Loon+, 2013)

NASA Astrophysics Data System (ADS)

van Loon, J. T.; Bailey, M.; Tatton, B. L.; Maíz Apellániz, J.; Crowther, P. A.; de Koter, A.; Evans, C. J.; Henault-Brunet, V.; Howarth, I. D.; Richter, P.; Sana, H.; Simon-Diaz, S.; Taylor, W.; Walborn, N. R.

2012-11-01

Equivalent widths of the 4428, 5780, 5797 and 6614 Diffuse Interstellar Bands (DIBs) were measured for up to 800 OB-type stars in the Tarantula Nebula (30 Doradus, containing the massive compact star cluster R136) in the Large Magellanic Cloud (LMC). The Galactic foreground and LMC components were fitted simultaneously, with Lorentzians for the 4428 DIB and Gaussians for the other DIBs. All fits were inspected by eye, and a flag was reset to zero if the fit was rejected. (3 data files).

HUBBLE TARANTULA TREASURY PROJECT. V. THE STAR CLUSTER HODGE 301: THE OLD FACE OF 30 DORADUS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cignoni, M.; Sabbi, E.; Marel, R. P. van der

Based on color–magnitude diagrams (CMDs) from the Hubble Space Telescope  Hubble Tarantula Treasury Project (HTTP) survey, we present the star formation history of Hodge 301, the oldest star cluster in the Tarantula Nebula. The HTTP photometry extends faint enough to reach, for the first time, the cluster pre-main sequence (PMS) turn-on, where the PMS joins the main sequence. Using the location of this feature, along with synthetic CMDs generated with the latest PARSEC models, we find that Hodge 301 is older than previously thought, with an age between 26.5 and 31.5 Myr. From this age, we also estimate that between 38 andmore » 61 Type II supernovae exploded in the region. The same age is derived from the main sequence turn-off, whereas the age derived from the post-main sequence stars is younger and between 20 and 25 Myr. Other relevant parameters are a total stellar mass of ≈8800 ± 800  M {sub ⊙} and average reddening E ( B − V ) ≈ 0.22–0.24 mag, with a differential reddening δE ( B − V ) ≈ 0.04 mag.« less

Hubble Tarantula Treasury Project V. The Star Cluster Hodge 301: The Old Face of 30 Doradus

NASA Astrophysics Data System (ADS)

Cignoni, M.; Sabbi, E.; van der Marel, R. P.; Lennon, D. J.; Tosi, M.; Grebel, E. K.; Gallagher, J. S., III; Aloisi, A.; de Marchi, G.; Gouliermis, D. A.; Larsen, S.; Panagia, N.; Smith, L. J.

2016-12-01

Based on color-magnitude diagrams (CMDs) from the Hubble Space Telescope Hubble Tarantula Treasury Project (HTTP) survey, we present the star formation history of Hodge 301, the oldest star cluster in the Tarantula Nebula. The HTTP photometry extends faint enough to reach, for the first time, the cluster pre-main sequence (PMS) turn-on, where the PMS joins the main sequence. Using the location of this feature, along with synthetic CMDs generated with the latest PARSEC models, we find that Hodge 301 is older than previously thought, with an age between 26.5 and 31.5 Myr. From this age, we also estimate that between 38 and 61 Type II supernovae exploded in the region. The same age is derived from the main sequence turn-off, whereas the age derived from the post-main sequence stars is younger and between 20 and 25 Myr. Other relevant parameters are a total stellar mass of ≈8800 ± 800 M ⊙ and average reddening E(B - V) ≈ 0.22-0.24 mag, with a differential reddening δE(B - V) ≈ 0.04 mag. Based on observations with the NASA/ESA Hubble Space Telescope, obtained at the Space Telescope Science Institute, which is operated by AURA Inc., under NASA contract NAS 5-26555.

Explosive Model Tarantula V1/JWL++ Calibration of LX-17: #2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Souers, P C; Vitello, P

2009-05-01

Tarantula V1 is a kinetic package for reactive flow codes that seeks to describe initiation, failure, dead zones and detonation simultaneously. The most important parameter is P1, the pressure between the initiation and failure regions. Both dead zone formation and failure can be largely controlled with this knob. However, V1 does failure with low settings and dead zones with higher settings, so that it cannot fulfill its purpose in the current format. To this end, V2 is under test. The derivation of the initiation threshold P0 is discussed. The derivation of the initiation pressure-tau curve as an output of Tarantulamore » shows that the initiation package is sound. A desensitization package is also considered.« less

Explosive Model Tarantula 4d/JWL++ Calibration of LX-17

DOE Office of Scientific and Technical Information (OSTI.GOV)

Souers, P C; Vitello, P A

2008-09-30

Tarantula is an explosive kinetic package intended to do detonation, shock initiation, failure, corner-turning with dead zones, gap tests and air gaps in reactive flow hydrocode models. The first, 2007-2008 version with monotonic Q is here run inside JWL++ with square zoning from 40 to 200 zones/cm on ambient LX-17. The model splits the rate behavior in every zone into sections set by the hydrocode pressure, P + Q. As the pressure rises, we pass through the no-reaction, initiation, ramp-up/failure and detonation sections sequentially. We find that the initiation and pure detonation rate constants are largely insensitive to zoning butmore » that the ramp-up/failure rate constant is extremely sensitive. At no time does the model pass every test, but the pressure-based approach generally works. The best values for the ramp/failure region are listed here in Mb units.« less

An Investigation of Amphitheater-Headed Canyon Distribution, Morphology Variation, and Longitudinal Profile Controls in Escalante and Tarantula Mesa, Utah.

NASA Astrophysics Data System (ADS)

Ryan, A. J.; Whipple, K. X.

2014-12-01

Amphitheater-headed canyons are primarily distinguished from typical fluvial channels by their abrupt headwall terminations. A key goal in the study of river canyons is to establish a reliable link between form and formation processes. This is of particular significance for Mars, where, if such links can be established, amphitheater-headed canyons could be used to determine ancient erosion mechanisms and, by inference, climate conditions. Type examples in arid regions on Earth, such as in Escalante River, Utah, previously have been interpreted as products of groundwater seepage erosion. We investigate amphitheater-headed canyons in Escalante and Tarantula Mesa where variations in canyon head morphology may hold clues for the relative roles of rock properties and fluvial and groundwater processes. In lower Escalante, amphitheaters are only present where canyons have breached the Navajo Sandstone - Kayenta Formation contact. In some canyons, amphitheater development appears to have been inhibited by an abundance of coarse bedload. In Tarantula Mesa, canyons have a variety of headwalls, from amphitheaters to stepped knickzones. Headwall morphology distribution is directly related to the spatially variable presence of knickpoint-forming, fine-grained interbeds within cliff-forming sandstones. Amphitheaters only form where the sandstone unit is undisrupted by these interbeds. Finally, most canyons in Escalante and Tarantula Mesa, regardless of substrate lithology, amphitheater presence, or groundwater spring intensity, are well described by a slope-area power law relationship with regionally constant concavity and normalized steepness indices. This suggests that all channels here are subject to the same erosion rates, independent of groundwater weathering intensity. Thus: 1) variations in canyon headwall form do not necessary relate to differences in fluvial history, 2) stratigraphic variations are clearly of importance in sedimentary canyon systems, and 3) although

Hubble Tarantula Treasury Project - VI. Identification of Pre-Main-Sequence Stars using Machine Learning techniques

NASA Astrophysics Data System (ADS)

Ksoll, Victor F.; Gouliermis, Dimitrios A.; Klessen, Ralf S.; Grebel, Eva K.; Sabbi, Elena; Anderson, Jay; Lennon, Daniel J.; Cignoni, Michele; de Marchi, Guido; Smith, Linda J.; Tosi, Monica; van der Marel, Roeland P.

2018-05-01

The Hubble Tarantula Treasury Project (HTTP) has provided an unprecedented photometric coverage of the entire star-burst region of 30 Doradus down to the half Solar mass limit. We use the deep stellar catalogue of HTTP to identify all the pre-main-sequence (PMS) stars of the region, i.e., stars that have not started their lives on the main-sequence yet. The photometric distinction of these stars from the more evolved populations is not a trivial task due to several factors that alter their colour-magnitude diagram positions. The identification of PMS stars requires, thus, sophisticated statistical methods. We employ Machine Learning Classification techniques on the HTTP survey of more than 800,000 sources to identify the PMS stellar content of the observed field. Our methodology consists of 1) carefully selecting the most probable low-mass PMS stellar population of the star-forming cluster NGC2070, 2) using this sample to train classification algorithms to build a predictive model for PMS stars, and 3) applying this model in order to identify the most probable PMS content across the entire Tarantula Nebula. We employ Decision Tree, Random Forest and Support Vector Machine classifiers to categorise the stars as PMS and Non-PMS. The Random Forest and Support Vector Machine provided the most accurate models, predicting about 20,000 sources with a candidateship probability higher than 50 percent, and almost 10,000 PMS candidates with a probability higher than 95 percent. This is the richest and most accurate photometric catalogue of extragalactic PMS candidates across the extent of a whole star-forming complex.

A novel tarantula toxin stabilizes the deactivated voltage sensor of bacterial sodium channel.

PubMed

Tang, Cheng; Zhou, Xi; Nguyen, Phuong Tran; Zhang, Yunxiao; Hu, Zhaotun; Zhang, Changxin; Yarov-Yarovoy, Vladimir; DeCaen, Paul G; Liang, Songping; Liu, Zhonghua

2017-07-01

Voltage-gated sodium channels (Na V s) are activated by transiting the voltage sensor from the deactivated to the activated state. The crystal structures of several bacterial Na V s have captured the voltage sensor module (VSM) in an activated state, but structure of the deactivated voltage sensor remains elusive. In this study, we sought to identify peptide toxins stabilizing the deactivated VSM of bacterial Na V s. We screened fractions from several venoms and characterized a cystine knot toxin called JZTx-27 from the venom of tarantula Chilobrachys jingzhao as a high-affinity antagonist of the prokaryotic Na V s Ns V Ba (nonselective voltage-gated Bacillus alcalophilus ) and NaChBac (bacterial sodium channel from Bacillus halodurans ) (IC 50 = 112 nM and 30 nM, respectively). JZTx-27 was more efficacious at weaker depolarizing voltages and significantly slowed the activation but accelerated the deactivation of Ns V Ba, whereas the local anesthetic drug lidocaine was shown to antagonize Ns V Ba without affecting channel gating. Mutation analysis confirmed that JZTx-27 bound to S3-4 linker of Ns V Ba, with F98 being the critical residue in determining toxin affinity. All electrophysiological data and in silico analysis suggested that JZTx-27 trapped VSM of Ns V Ba in one of the deactivated states. In mammalian Na V s, JZTx-27 preferably inhibited the inactivation of Na V 1.5 by targeting the fourth transmembrane domain. To our knowledge, this is the first report of peptide antagonist for prokaryotic Na V s. More important, we proposed that JZTx-27 stabilized the Ns V Ba VSM in the deactivated state and may be used as a probe to determine the structure of the deactivated VSM of Na V s.-Tang, C., Zhou, X., Nguyen, P. T., Zhang, Y., Hu, Z., Zhang, C., Yarov-Yarovoy, V., DeCaen, P. G., Liang, S., Liu, Z. A novel tarantula toxin stabilizes the deactivated voltage sensor of bacterial sodium channel. © FASEB.

Tarantula huwentoxin-IV inhibits neuronal sodium channels by binding to receptor site 4 and trapping the domain ii voltage sensor in the closed configuration.

PubMed

Xiao, Yucheng; Bingham, Jon-Paul; Zhu, Weiguo; Moczydlowski, Edward; Liang, Songping; Cummins, Theodore R

2008-10-03

Peptide toxins with high affinity, divergent pharmacological functions, and isoform-specific selectivity are powerful tools for investigating the structure-function relationships of voltage-gated sodium channels (VGSCs). Although a number of interesting inhibitors have been reported from tarantula venoms, little is known about the mechanism for their interaction with VGSCs. We show that huwentoxin-IV (HWTX-IV), a 35-residue peptide from tarantula Ornithoctonus huwena venom, preferentially inhibits neuronal VGSC subtypes rNav1.2, rNav1.3, and hNav1.7 compared with muscle subtypes rNav1.4 and hNav1.5. Of the five VGSCs examined, hNav1.7 was most sensitive to HWTX-IV (IC(50) approximately 26 nM). Following application of 1 microm HWTX-IV, hNav1.7 currents could only be elicited with extreme depolarizations (>+100 mV). Recovery of hNav1.7 channels from HWTX-IV inhibition could be induced by extreme depolarizations or moderate depolarizations lasting several minutes. Site-directed mutagenesis analysis indicated that the toxin docked at neurotoxin receptor site 4 located at the extracellular S3-S4 linker of domain II. Mutations E818Q and D816N in hNav1.7 decreased toxin affinity for hNav1.7 by approximately 300-fold, whereas the reverse mutations in rNav1.4 (N655D/Q657E) and the corresponding mutations in hNav1.5 (R812D/S814E) greatly increased the sensitivity of the muscle VGSCs to HWTX-IV. Our data identify a novel mechanism for sodium channel inhibition by tarantula toxins involving binding to neurotoxin receptor site 4. In contrast to scorpion beta-toxins that trap the IIS4 voltage sensor in an outward configuration, we propose that HWTX-IV traps the voltage sensor of domain II in the inward, closed configuration.

The VLT-FLAMES Tarantula Survey. IX. The interstellar medium seen through diffuse interstellar bands and neutral sodium&

NASA Astrophysics Data System (ADS)

van Loon, J. Th.; Bailey, M.; Tatton, B. L.; Maíz Apellániz, J.; Crowther, P. A.; de Koter, A.; Evans, C. J.; Hénault-Brunet, V.; Howarth, I. D.; Richter, P.; Sana, H.; Simón-Díaz, S.; Taylor, W.; Walborn, N. R.

2013-02-01

Context. The Tarantula Nebula (a.k.a. 30 Dor) is a spectacular star-forming region in the Large Magellanic Cloud (LMC), seen through gas in the Galactic disc and halo. Diffuse interstellar bands (DIBs) offer a unique probe of the diffuse, cool-warm gas in these regions. Aims: The aim is to use DIBs as diagnostics of the local interstellar conditions, whilst at the same time deriving properties of the yet-unknown carriers of these enigmatic spectral features. Methods: Spectra of over 800 early-type stars from the Very Large Telescope Flames Tarantula Survey (VFTS) were analysed. Maps were created, separately, for the Galactic and LMC absorption in the DIBs at 4428 and 6614 Å and - in a smaller region near the central cluster R 136 - neutral sodium (the Na i D doublet); we also measured the DIBs at 5780 and 5797 Å. Results: The maps show strong 4428 and 6614 Å DIBs in the quiescent cloud complex to the south of 30 Dor but weak absorption in the harsher environments to the north (bubbles) and near the OB associations. The Na maps show at least five kinematic components in the LMC and a shell-like structure surrounding R 136, and small-scale structure in the Milky Way. The strengths of the 4428, 5780, 5797 and 6614 Å DIBs are correlated, also with Na absorption and visual extinction. The strong 4428 Å DIB is present already at low Na column density but the 6614, 5780 and 5797 Å DIBs start to be detectable at subsequently larger Na column densities. Conclusions: The carriers of the 4428, 6614, 5780 and 5797 Å DIBs are increasingly prone to removal from irradiated gas. The relative strength of the 5780 and 5797 Å DIBs clearly confirm the Tarantula Nebula as well as Galactic high-latitude gas to represent a harsh radiation environment. The resilience of the 4428 Å DIB suggests its carrier is large, compact and neutral. Structure is detected in the distribution of cool-warm gas on scales between one and >100 pc in the LMC and as little as 0.01 pc in the Sun

Morphological and Color Differences between Island and Mainland Populations in the Mexican Red Rump Tarantula, Brachypelma vagans

PubMed Central

Vilchis-Nestor, Claudia A.; Machkour-M'Rabet, Salima; Barriga-Sosa, Irene de los A.; Winterton, Peter; Hénaut, Yann

2013-01-01

The introduction of species into new ecosystems, especially in small and isolated regions such as islands, offers an excellent opportunity to answer questions of the evolutionary processes occurring in natural conditions on a scale that could never be achieved in laboratory conditions. In this study, we examined the Mexican red rump tarantula Brachypelma vagans Ausserer (Mygalomorphae: Theraphosidae), a species that was introduced to Cozumel Island, Mexico, 40 years ago. This introduction provides an exceptional model to study effects such as morphological variation between island populations and those on the mainland in open habitats facing the island. Intraspecific variation related to the color polymorphism was compared. The aim of this study was to determine the phenotypic differences between continental populations of B. vagans and the introduced population on Cozumel Island. Phenotypic difference was evaluated using two approaches: 1) comparison of the morphometric measurements of adult and juvenile individuals at the local scale and between continental and island populations, and 2) comparison of individual color polymorphism between mainland and island populations. Two locations were sampled within the continental part of the Yucatan peninsula and two on the island of Cozumel. The number of samples analyzed at each site was 30 individuals. The morphometric results showed significant differences between continental and island populations, with bigger individuals on the island. In addition, three new variations of the typical color pattern of B. vagans recorded so far were observed. This study opens the door to further investigations to elucidate the origin of the phenotypic variation of the isolated individuals on Cozumel Island. Also, the widest range of color morphs found for a tarantula species is reported. PMID:24224805

The VLT-FLAMES Tarantula Survey. VIII. Multiplicity properties of the O-type star population

NASA Astrophysics Data System (ADS)

Sana, H.; de Koter, A.; de Mink, S. E.; Dunstall, P. R.; Evans, C. J.; Hénault-Brunet, V.; Maíz Apellániz, J.; Ramírez-Agudelo, O. H.; Taylor, W. D.; Walborn, N. R.; Clark, J. S.; Crowther, P. A.; Herrero, A.; Gieles, M.; Langer, N.; Lennon, D. J.; Vink, J. S.

2013-02-01

Context. The Tarantula Nebula in the Large Magellanic Cloud is our closest view of a starburst region and is the ideal environment to investigate important questions regarding the formation, evolution and final fate of the most massive stars. Aims: We analyze the multiplicity properties of the massive O-type star population observed through multi-epoch spectroscopy in the framework of the VLT-FLAMES Tarantula Survey. With 360 O-type stars, this is the largest homogeneous sample of massive stars analyzed to date. Methods: We use multi-epoch spectroscopy and variability analysis to identify spectroscopic binaries. We also use a Monte-Carlo method to correct for observational biases. By modeling simultaneously the observed binary fraction, the distributions of the amplitudes of the radial velocity variations and the distribution of the time scales of these variations, we constrain the intrinsic current binary fraction and period and mass-ratio distributions. Results: We observe a spectroscopic binary fraction of 0.35 ± 0.03, which corresponds to the fraction of objects displaying statistically significant radial velocity variations with an amplitude of at least 20 km s-1. We compute the intrinsic binary fraction to be 0.51 ± 0.04. We adopt power-laws to describe the intrinsic period and mass-ratio distributions: f(log 10P/d) ~ (log 10P/d)π (with log 10P/d in the range 0.15-3.5) and f(q) ~ qκ with 0.1 ≤ q = M2/M1 ≤ 1.0. The power-law indexes that best reproduce the observed quantities are π = -0.45 ± 0.30 and κ = -1.0 ± 0.4. The period distribution that we obtain thus favours shorter period systems compared to an Öpik law (π = 0). The mass ratio distribution is slightly skewed towards low mass ratio systems but remains incompatible with a random sampling of a classical mass function (κ = -2.35). The binary fraction seems mostly uniform across the field of view and independent of the spectral types and luminosity classes. The binary fraction in the outer

The VLT-FLAMES Tarantula Survey. I. Introduction and observational overview

NASA Astrophysics Data System (ADS)

Evans, C. J.; Taylor, W. D.; Hénault-Brunet, V.; Sana, H.; de Koter, A.; Simón-Díaz, S.; Carraro, G.; Bagnoli, T.; Bastian, N.; Bestenlehner, J. M.; Bonanos, A. Z.; Bressert, E.; Brott, I.; Campbell, M. A.; Cantiello, M.; Clark, J. S.; Costa, E.; Crowther, P. A.; de Mink, S. E.; Doran, E.; Dufton, P. L.; Dunstall, P. R.; Friedrich, K.; Garcia, M.; Gieles, M.; Gräfener, G.; Herrero, A.; Howarth, I. D.; Izzard, R. G.; Langer, N.; Lennon, D. J.; Maíz Apellániz, J.; Markova, N.; Najarro, F.; Puls, J.; Ramirez, O. H.; Sabín-Sanjulián, C.; Smartt, S. J.; Stroud, V. E.; van Loon, J. Th.; Vink, J. S.; Walborn, N. R.

2011-06-01

The VLT-FLAMES Tarantula Survey (VFTS) is an ESO Large Programme that has obtained multi-epoch optical spectroscopy of over 800 massive stars in the 30 Doradus region of the Large Magellanic Cloud (LMC). Here we introduce our scientific motivations and give an overview of the survey targets, including optical and near-infrared photometry and comprehensive details of the data reduction. One of the principal objectives was to detect massive binary systems via variations in their radial velocities, thus shaping the multi-epoch observing strategy. Spectral classifications are given for the massive emission-line stars observed by the survey, including the discovery of a new Wolf-Rayet star (VFTS 682, classified as WN5h), 2' to the northeast of R136. To illustrate the diversity of objects encompassed by the survey, we investigate the spectral properties of sixteen targets identified by Gruendl & Chu from Spitzer photometry as candidate young stellar objects or stars with notable mid-infrared excesses. Detailed spectral classification and quantitative analysis of the O- and B-type stars in the VFTS sample, paying particular attention to the effects of rotational mixing and binarity, will be presented in a series of future articles to address fundamental questions in both stellar and cluster evolution. Figures 10-12, Tables 5 and 6, and Appendix A are available in electronic form at http://www.aanda.org

Molecular Interactions between Tarantula Toxins and Low-Voltage-Activated Calcium Channels

PubMed Central

Salari, Autoosa; Vega, Benjamin S.; Milescu, Lorin S.; Milescu, Mirela

2016-01-01

Few gating-modifier toxins have been reported to target low-voltage-activated (LVA) calcium channels, and the structural basis of toxin sensitivity remains incompletely understood. Studies of voltage-gated potassium (Kv) channels have identified the S3b–S4 “paddle motif,” which moves at the protein-lipid interface to drive channel opening, as the target for these amphipathic neurotoxins. Voltage-gated calcium (Cav) channels contain four homologous voltage sensor domains, suggesting multiple toxin binding sites. We show here that the S3–S4 segments within Cav3.1 can be transplanted into Kv2.1 to examine their individual contributions to voltage sensing and pharmacology. With these results, we now have a more complete picture of the conserved nature of the paddle motif in all three major voltage-gated ion channel types (Kv, Nav, and Cav). When screened with tarantula toxins, the four paddle sequences display distinct toxin binding properties, demonstrating that gating-modifier toxins can bind to Cav channels in a domain specific fashion. Domain III was the most commonly and strongly targeted, and mutagenesis revealed an acidic residue that is important for toxin binding. We also measured the lipid partitioning strength of all toxins tested and observed a positive correlation with their inhibition of Cav3.1, suggesting a key role for membrane partitioning. PMID:27045173

The VLT-FLAMES Tarantula Survey. XXVII. Physical parameters of B-type main-sequence binary systems in the Tarantula nebula

NASA Astrophysics Data System (ADS)

Garland, R.; Dufton, P. L.; Evans, C. J.; Crowther, P. A.; Howarth, I. D.; de Koter, A.; de Mink, S. E.; Grin, N. J.; Langer, N.; Lennon, D. J.; McEvoy, C. M.; Sana, H.; Schneider, F. R. N.; Símon Díaz, S.; Taylor, W. D.; Thompson, A.; Vink, J. S.

2017-07-01

A spectroscopic analysis has been undertaken for the B-type multiple systems (excluding those with supergiant primaries) in the VLT-FLAMES Tarantula Survey (VFTS). Projected rotational velocities, vesini, for the primaries have been estimated using a Fourier Transform technique and confirmed by fitting rotationally broadened profiles. A subset of 33 systems with vesini ≤ 80 km s-1 have been analysed using a TLUSTY grid of model atmospheres to estimate stellar parameters and surface abundances for the primaries. The effects of a potential flux contribution from an unseen secondary have also been considered. For 20 targets it was possible to reliably estimate their effective temperatures (Teff) but for the other 13 objects it was only possible to provide a constraint of 20 000 ≤ Teff ≤ 26 000 K - the other parameters estimated for these targets will be consequently less reliable. The estimated stellar properties are compared with evolutionary models and are generally consistent with their membership of 30 Doradus, while the nature of the secondaries of 3 SB2 system is discussed. A comparison with a sample of single stars with vesini ≤ 80 km s-1 obtained from the VFTS and analysed with the same techniques implies that the atmospheric parameters and nitrogen abundances of the two samples are similar. However, the binary sample may have a lack of primaries with significant nitrogen enhancements, which would be consistent with them having low rotational velocities and having effectively evolved as single stars without significant rotational mixing. This result, which may be actually a consequence of the limitations of the pathfinder investigation presented in this paper, should be considered as a motivation for spectroscopic abundance analysis of large samples of binary stars, with high quality observational data. Based on observations collected at the European Organisation for Astronomical Research in the Southern Hemisphere under ESO programme 182.D-0222.Tables

Pharmacological characterization of potent and selective NaV1.7 inhibitors engineered from Chilobrachys jingzhao tarantula venom peptide JzTx-V.

PubMed

Moyer, Bryan D; Murray, Justin K; Ligutti, Joseph; Andrews, Kristin; Favreau, Philippe; Jordan, John B; Lee, Josie H; Liu, Dong; Long, Jason; Sham, Kelvin; Shi, Licheng; Stöcklin, Reto; Wu, Bin; Yin, Ruoyuan; Yu, Violeta; Zou, Anruo; Biswas, Kaustav; Miranda, Les P

2018-01-01

Identification of voltage-gated sodium channel NaV1.7 inhibitors for chronic pain therapeutic development is an area of vigorous pursuit. In an effort to identify more potent leads compared to our previously reported GpTx-1 peptide series, electrophysiology screening of fractionated tarantula venom discovered the NaV1.7 inhibitory peptide JzTx-V from the Chinese earth tiger tarantula Chilobrachys jingzhao. The parent peptide displayed nominal selectivity over the skeletal muscle NaV1.4 channel. Attribute-based positional scan analoging identified a key Ile28Glu mutation that improved NaV1.4 selectivity over 100-fold, and further optimization yielded the potent and selective peptide leads AM-8145 and AM-0422. NMR analyses revealed that the Ile28Glu substitution changed peptide conformation, pointing to a structural rationale for the selectivity gains. AM-8145 and AM-0422 as well as GpTx-1 and HwTx-IV competed for ProTx-II binding in HEK293 cells expressing human NaV1.7, suggesting that these NaV1.7 inhibitory peptides interact with a similar binding site. AM-8145 potently blocked native tetrodotoxin-sensitive (TTX-S) channels in mouse dorsal root ganglia (DRG) neurons, exhibited 30- to 120-fold selectivity over other human TTX-S channels and exhibited over 1,000-fold selectivity over other human tetrodotoxin-resistant (TTX-R) channels. Leveraging NaV1.7-NaV1.5 chimeras containing various voltage-sensor and pore regions, AM-8145 mapped to the second voltage-sensor domain of NaV1.7. AM-0422, but not the inactive peptide analog AM-8374, dose-dependently blocked capsaicin-induced DRG neuron action potential firing using a multi-electrode array readout and mechanically-induced C-fiber spiking in a saphenous skin-nerve preparation. Collectively, AM-8145 and AM-0422 represent potent, new engineered NaV1.7 inhibitory peptides derived from the JzTx-V scaffold with improved NaV selectivity and biological activity in blocking action potential firing in both DRG neurons and C-fibers.

Pharmacological characterization of potent and selective NaV1.7 inhibitors engineered from Chilobrachys jingzhao tarantula venom peptide JzTx-V

PubMed Central

Murray, Justin K.; Ligutti, Joseph; Andrews, Kristin; Favreau, Philippe; Jordan, John B.; Lee, Josie H.; Liu, Dong; Long, Jason; Sham, Kelvin; Shi, Licheng; Stöcklin, Reto; Wu, Bin; Yin, Ruoyuan; Yu, Violeta; Zou, Anruo; Biswas, Kaustav; Miranda, Les P.

2018-01-01

Identification of voltage-gated sodium channel NaV1.7 inhibitors for chronic pain therapeutic development is an area of vigorous pursuit. In an effort to identify more potent leads compared to our previously reported GpTx-1 peptide series, electrophysiology screening of fractionated tarantula venom discovered the NaV1.7 inhibitory peptide JzTx-V from the Chinese earth tiger tarantula Chilobrachys jingzhao. The parent peptide displayed nominal selectivity over the skeletal muscle NaV1.4 channel. Attribute-based positional scan analoging identified a key Ile28Glu mutation that improved NaV1.4 selectivity over 100-fold, and further optimization yielded the potent and selective peptide leads AM-8145 and AM-0422. NMR analyses revealed that the Ile28Glu substitution changed peptide conformation, pointing to a structural rationale for the selectivity gains. AM-8145 and AM-0422 as well as GpTx-1 and HwTx-IV competed for ProTx-II binding in HEK293 cells expressing human NaV1.7, suggesting that these NaV1.7 inhibitory peptides interact with a similar binding site. AM-8145 potently blocked native tetrodotoxin-sensitive (TTX-S) channels in mouse dorsal root ganglia (DRG) neurons, exhibited 30- to 120-fold selectivity over other human TTX-S channels and exhibited over 1,000-fold selectivity over other human tetrodotoxin-resistant (TTX-R) channels. Leveraging NaV1.7-NaV1.5 chimeras containing various voltage-sensor and pore regions, AM-8145 mapped to the second voltage-sensor domain of NaV1.7. AM-0422, but not the inactive peptide analog AM-8374, dose-dependently blocked capsaicin-induced DRG neuron action potential firing using a multi-electrode array readout and mechanically-induced C-fiber spiking in a saphenous skin-nerve preparation. Collectively, AM-8145 and AM-0422 represent potent, new engineered NaV1.7 inhibitory peptides derived from the JzTx-V scaffold with improved NaV selectivity and biological activity in blocking action potential firing in both DRG neurons and C

Different Head Environments in Tarantula Thick Filaments Support a Cooperative Activation Process

PubMed Central

Sulbarán, Guidenn; Biasutto, Antonio; Alamo, Lorenzo; Riggs, Claire; Pinto, Antonio; Méndez, Franklin; Craig, Roger; Padrón, Raúl

2013-01-01

Myosin filaments from many muscles are activated by phosphorylation of their regulatory light chains (RLCs). Structural analysis of relaxed tarantula thick filaments shows that the RLCs of the interacting free and blocked myosin heads are in different environments. This and other data suggested a phosphorylation mechanism in which Ser-35 of the free head is exposed and constitutively phosphorylated by protein kinase C, whereas the blocked head is hidden and unphosphorylated; on activation, myosin light chain kinase phosphorylates the monophosphorylated free head followed by the unphosphorylated blocked head, both at Ser-45. Our goal was to test this model of phosphorylation. Mass spectrometry of quickly frozen, intact muscles showed that only Ser-35 was phosphorylated in the relaxed state. The location of this constitutively phosphorylated Ser-35 was analyzed by immunofluorescence, using antibodies specific for unphosphorylated or phosphorylated Ser-35. In the relaxed state, myofibrils were labeled by anti-pSer-35 but not by anti-Ser-35, whereas in rigor, labeling was similar with both. This suggests that only pSer-35 is exposed in the relaxed state, while in rigor, Ser-35 is also exposed. In the interacting-head motif of relaxed filaments, only the free head RLCs are exposed, suggesting that the constitutive pSer-35 is on the free heads, consistent with the proposed mechanism. PMID:24209856

The Tarantula Nebula as a template for extragalactic star forming regions from VLT/MUSE and HST/STIS

NASA Astrophysics Data System (ADS)

Crowther, Paul A.; Caballero-Nieves, Saida M.; Castro, Norberto; Evans, Christopher J.

2017-11-01

We present VLT/MUSE observations of NGC 2070, the dominant ionizing nebula of 30 Doradus in the LMC, plus HST/STIS spectroscopy of its central star cluster R136. Integral Field Spectroscopy (MUSE) and pseudo IFS (STIS) together provides a complete census of all massive stars within the central 30×30 parsec2 of the Tarantula. We discuss the integrated far-UV spectrum of R136, of particular interest for UV studies of young extragalactic star clusters. Strong He iiλ1640 emission at very early ages (1-2 Myr) from very massive stars cannot be reproduced by current population synthesis models, even those incorporating binary evolution and very massive stars. A nebular analysis of the integrated MUSE dataset implies an age of ~4.5 Myr for NGC 2070. Wolf-Rayet features provide alternative age diagnostics, with the primary contribution to the integrated Wolf-Rayet bumps arising from R140 rather than the more numerous H-rich WN stars in R136. Caution should be used when interpreting spatially extended observations of extragalactic star-forming regions.

Structural insights into the mechanism of activation of the TRPV1 channel by a membrane-bound tarantula toxin

PubMed Central

Bae, Chanhyung; Anselmi, Claudio; Kalia, Jeet; Jara-Oseguera, Andres; Schwieters, Charles D; Krepkiy, Dmitriy; Won Lee, Chul; Kim, Eun-Hee; Kim, Jae Il; Faraldo-Gómez, José D; Swartz, Kenton J

2016-01-01

Venom toxins are invaluable tools for exploring the structure and mechanisms of ion channels. Here, we solve the structure of double-knot toxin (DkTx), a tarantula toxin that activates the heat-activated TRPV1 channel. We also provide improved structures of TRPV1 with and without the toxin bound, and investigate the interactions of DkTx with the channel and membranes. We find that DkTx binds to the outer edge of the external pore of TRPV1 in a counterclockwise configuration, using a limited protein-protein interface and inserting hydrophobic residues into the bilayer. We also show that DkTx partitions naturally into membranes, with the two lobes exhibiting opposing energetics for membrane partitioning and channel activation. Finally, we find that the toxin disrupts a cluster of hydrophobic residues behind the selectivity filter that are critical for channel activation. Collectively, our findings reveal a novel mode of toxin-channel recognition that has important implications for the mechanism of thermosensation. DOI: http://dx.doi.org/10.7554/eLife.11273.001 PMID:26880553

The VLT-FLAMES Tarantula Survey. XII. Rotational velocities of the single O-type stars

NASA Astrophysics Data System (ADS)

Ramírez-Agudelo, O. H.; Simón-Díaz, S.; Sana, H.; de Koter, A.; Sabín-Sanjulían, C.; de Mink, S. E.; Dufton, P. L.; Gräfener, G.; Evans, C. J.; Herrero, A.; Langer, N.; Lennon, D. J.; Maíz Apellániz, J.; Markova, N.; Najarro, F.; Puls, J.; Taylor, W. D.; Vink, J. S.

2013-12-01

Context. The 30 Doradus (30 Dor) region of the Large Magellanic Cloud, also known as the Tarantula nebula, is the nearest starburst region. It contains the richest population of massive stars in the Local Group, and it is thus the best possible laboratory to investigate open questions on the formation and evolution of massive stars. Aims: Using ground-based multi-object optical spectroscopy obtained in the framework of the VLT-FLAMES Tarantula Survey (VFTS), we aim to establish the (projected) rotational velocity distribution for a sample of 216 presumably single O-type stars in 30 Dor. The sample is large enough to obtain statistically significant information and to search for variations among subpopulations - in terms of spectral type, luminosity class, and spatial location - in the field of view. Methods: We measured projected rotational velocities, νesini, by means of a Fourier transform method and a profile fitting method applied to a set of isolated spectral lines. We also used an iterative deconvolution procedure to infer the probability density, P(νe), of the equatorial rotational velocity, νe. Results: The distribution of νesini shows a two-component structure: a peak around 80 kms-1 and a high-velocity tail extending up to ~600 kms-1. This structure is also present in the inferred distribution P(νe) with around 80% of the sample having 0 < νe ≤ 300 kms-1 and the other 20% distributed in the high-velocity region. The presence of the low-velocity peak is consistent with what has been found in other studies for late O- and early B-type stars. Conclusions: Most of the stars in our sample rotate with a rate less than 20% of their break-up velocity. For the bulk of the sample, mass loss in a stellar wind and/or envelope expansion is not efficient enough to significantly spin down these stars within the first few Myr of evolution. If massive-star formation results in stars rotating at birth with a large portion of their break-up velocities, an

Chemoselective tarantula toxins report voltage activation of wild-type ion channels in live cells

PubMed Central

Tilley, Drew C.; Eum, Kenneth S.; Fletcher-Taylor, Sebastian; Austin, Daniel C.; Dupré, Christophe; Patrón, Lilian A.; Garcia, Rita L.; Lam, Kit; Yarov-Yarovoy, Vladimir; Cohen, Bruce E.; Sack, Jon T.

2014-01-01

Electrically excitable cells, such as neurons, exhibit tremendous diversity in their firing patterns, a consequence of the complex collection of ion channels present in any specific cell. Although numerous methods are capable of measuring cellular electrical signals, understanding which types of ion channels give rise to these signals remains a significant challenge. Here, we describe exogenous probes which use a novel mechanism to report activity of voltage-gated channels. We have synthesized chemoselective derivatives of the tarantula toxin guangxitoxin-1E (GxTX), an inhibitory cystine knot peptide that binds selectively to Kv2-type voltage gated potassium channels. We find that voltage activation of Kv2.1 channels triggers GxTX dissociation, and thus GxTX binding dynamically marks Kv2 activation. We identify GxTX residues that can be replaced by thiol- or alkyne-bearing amino acids, without disrupting toxin folding or activity, and chemoselectively ligate fluorophores or affinity probes to these sites. We find that GxTX–fluorophore conjugates colocalize with Kv2.1 clusters in live cells and are released from channels activated by voltage stimuli. Kv2.1 activation can be detected with concentrations of probe that have a trivial impact on cellular currents. Chemoselective GxTX mutants conjugated to dendrimeric beads likewise bind live cells expressing Kv2.1, and the beads are released by channel activation. These optical sensors of conformational change are prototype probes that can indicate when ion channels contribute to electrical signaling. PMID:25331865

Lessons from a tarantula: new insights into myosin interacting-heads motif evolution and its implications on disease.

PubMed

Alamo, Lorenzo; Pinto, Antonio; Sulbarán, Guidenn; Mavárez, Jesús; Padrón, Raúl

2017-09-04

Tarantula's leg muscle thick filament is the ideal model for the study of the structure and function of skeletal muscle thick filaments. Its analysis has given rise to a series of structural and functional studies, leading, among other things, to the discovery of the myosin interacting-heads motif (IHM). Further electron microscopy (EM) studies have shown the presence of IHM in frozen-hydrated and negatively stained thick filaments of striated, cardiac, and smooth muscle of bilaterians, most showing the IHM parallel to the filament axis. EM studies on negatively stained heavy meromyosin of different species have shown the presence of IHM on sponges, animals that lack muscle, extending the presence of IHM to metazoans. The IHM evolved about 800 MY ago in the ancestor of Metazoa, and independently with functional differences in the lineage leading to the slime mold Dictyostelium discoideum (Mycetozoa). This motif conveys important functional advantages, such as Ca 2+ regulation and ATP energy-saving mechanisms. Recent interest has focused on human IHM structure in order to understand the structural basis underlying various conditions and situations of scientific and medical interest: the hypertrophic and dilated cardiomyopathies, overfeeding control, aging and hormone deprival muscle weakness, drug design for schistosomiasis control, and conditioning exercise physiology for the training of power athletes.

Synergetic Action of Domain II and IV Underlies Persistent Current Generation in Nav1.3 as revealed by a tarantula toxin

PubMed Central

Tang, Cheng; Zhou, Xi; Zhang, Yunxiao; xiao, Zhaohua; Hu, Zhaotun; Zhang, Changxin; Huang, Ying; Chen, Bo; Liu, Zhonghua; Liang, Songping

2015-01-01

The persistent current (INaP) through voltage-gated sodium channels enhances neuronal excitability by causing prolonged depolarization of membranes. Nav1.3 intrinsically generates a small INaP, although the mechanism underlying its generation remains unclear. In this study, the involvement of the four domains of Nav1.3 in INaP generation was investigated using the tarantula toxin α-hexatoxin-MrVII (RTX-VII). RTX-VII activated Nav1.3 and induced a large INaP. A pre-activated state binding model was proposed to explain the kinetics of toxin-channel interaction. Of the four domains of Nav1.3, both domain II and IV might play important roles in the toxin-induced INaP. Domain IV constructed the binding site for RTX-VII, while domain II might not participate in interacting with RTX-VII but could determine the efficacy of RTX-VII. Our results based on the use of RTX-VII as a probe suggest that domain II and IV cooperatively contribute to the generation of INaP in Nav1.3. PMID:25784299

Isolation of an Orally Active Insecticidal Toxin from the Venom of an Australian Tarantula

PubMed Central

Hardy, Margaret C.; Daly, Norelle L.; Mobli, Mehdi; Morales, Rodrigo A. V.; King, Glenn F.

2013-01-01

Many insect pests have developed resistance to existing chemical insecticides and consequently there is much interest in the development of new insecticidal compounds with novel modes of action. Although spiders have deployed insecticidal toxins in their venoms for over 250 million years, there is no evolutionary selection pressure on these toxins to possess oral activity since they are injected into prey and predators via a hypodermic needle-like fang. Thus, it has been assumed that spider-venom peptides are not orally active and are therefore unlikely to be useful insecticides. Contrary to this dogma, we show that it is possible to isolate spider-venom peptides with high levels of oral insecticidal activity by directly screening for per os toxicity. Using this approach, we isolated a 34-residue orally active insecticidal peptide (OAIP-1) from venom of the Australian tarantula Selenotypus plumipes. The oral LD50 for OAIP-1 in the agronomically important cotton bollworm Helicoverpa armigera was 104.2±0.6 pmol/g, which is the highest per os activity reported to date for an insecticidal venom peptide. OAIP-1 is equipotent with synthetic pyrethroids and it acts synergistically with neonicotinoid insecticides. The three-dimensional structure of OAIP-1 determined using NMR spectroscopy revealed that the three disulfide bonds form an inhibitor cystine knot motif; this structural motif provides the peptide with a high level of biological stability that probably contributes to its oral activity. OAIP-1 is likely to be synergized by the gut-lytic activity of the Bacillus thuringiensis Cry toxin (Bt) expressed in insect-resistant transgenic crops, and consequently it might be a good candidate for trait stacking with Bt. PMID:24039872

Mapping the interaction site for the tarantula toxin hainantoxin-IV (β-TRTX-Hn2a) in the voltage sensor module of domain II of voltage-gated sodium channels.

PubMed

Cai, Tianfu; Luo, Ji; Meng, Er; Ding, Jiuping; Liang, Songping; Wang, Sheng; Liu, Zhonghua

2015-06-01

Peptide toxins often have pharmacological applications and are powerful tools for investigating the structure-function relationships of voltage-gated sodium channels (VGSCs). Although a group of potential VGSC inhibitors have been reported from tarantula venoms, little is known about the mechanism of their interaction with VGSCs. In this study, we showed that hainantoxin-IV (β-TRTX-Hn2a, HNTX-IV in brief), a 35-residue peptide from Ornithoctonus hainana venom, preferentially inhibited rNav1.2, rNav1.3 and hNav1.7 compared with rNav1.4 and hNav1.5. hNav1.7 was the most sensitive to HNTX-IV (IC50∼21nM). In contrast to many other tarantula toxins that affect VGSCs, HNTX-IV at subsaturating concentrations did not alter activation and inactivation kinetics in the physiological range of voltages, while very large depolarization above +70mV could partially activate toxin-bound hNav1.7 channel, indicating that HNTX-IV acts as a gating modifier rather than a pore blocker. Site-directed mutagenesis indicated that the toxin bound to site 4, which was located on the extracellular S3-S4 linker of hNav1.7 domain II. Mutants E753Q, D816N and E818Q of hNav1.7 decreased toxin affinity for hNav1.7 by 2.0-, 3.3- and 130-fold, respectively. In silico docking indicated that a three-toed claw substructure formed by residues with close contacts in the interface between HNTX-IV and hNav1.7 domain II stabilized the toxin-channel complex, impeding movement of the domain II voltage sensor and inhibiting hNav1.7 activation. Our data provide structural details for structure-based drug design and a useful template for the design of highly selective inhibitors of a specific subtype of VGSCs. Copyright © 2014 Elsevier Inc. All rights reserved.

A new model for the surface arrangement of myosin molecules in tarantula thick filaments.

PubMed

Offer, G; Knight, P J; Burgess, S A; Alamo, L; Padrón, R

2000-04-28

Three-dimensional reconstructions of the negatively stained thick filaments of tarantula muscle with a resolution of 50 A have previously suggested that the helical tracks of myosin heads are zigzagged, short diagonal ridges being connected by nearly axial links. However, surface views of lower contour levels reveal an additional J-shaped feature approximately the size and shape of a myosin head. We have modelled the surface array of myosin heads on the filaments using as a building block a model of a two-headed regulated myosin molecule in which the regulatory light chains of the two heads together form a compact head-tail junction. Four parameters defining the radius, orientation and rotation of each myosin molecule were varied. In addition, the heads were allowed independently to bend in a plane perpendicular to the coiled-coil tail at three sites, and to tilt with respect to the tail and to twist at one of these sites. After low-pass filtering, models were aligned with the reconstruction, scored by cross-correlation and refined by simulated annealing. Comparison of the geometry of the reconstruction and the distance between domains in the myosin molecule narrowed the choice of models to two main classes. A good match to the reconstruction was obtained with a model in which each ridge is formed from the motor domain of a head pointing to the bare zone together with the head-tail junction of a neighbouring molecule. The heads pointing to the Z-disc intermittently occupy the J-position. Each motor domain interacts with the essential and regulatory light chains of the neighbouring heads. A near-radial spoke in the reconstruction connecting the backbone to one end of the ridge can be identified as the start of the coiled-coil tail. Copyright 2000 Academic Press.

Discovery of a Distinct Superfamily of Kunitz-Type Toxin (KTT) from Tarantulas

PubMed Central

Diao, Jian-Bo; Jiang, Li-Ping; Tang, Xing; Liang, Song-Ping

2008-01-01

Background Kuntiz-type toxins (KTTs) have been found in the venom of animals such as snake, cone snail and sea anemone. The main ancestral function of Kunitz-type proteins was the inhibition of a diverse array of serine proteases, while toxic activities (such as ion-channel blocking) were developed under a variety of Darwinian selection pressures. How new functions were grafted onto an old protein scaffold and what effect Darwinian selection pressures had on KTT evolution remains a puzzle. Principal Findings Here we report the presence of a new superfamily of KTTs in spiders (Tarantulas: Ornithoctonus huwena and Ornithoctonus hainana), which share low sequence similarity to known KTTs and is clustered in a distinct clade in the phylogenetic tree of KTT evolution. The representative molecule of spider KTTs, HWTX-XI, purified from the venom of O. huwena, is a bi-functional protein which is a very potent trypsin inhibitor (about 30-fold more strong than BPTI) as well as a weak Kv1.1 potassium channel blocker. Structural analysis of HWTX-XI in 3-D by NMR together with comparative function analysis of 18 expressed mutants of this toxin revealed two separate sites, corresponding to these two activities, located on the two ends of the cone-shape molecule of HWTX-XI. Comparison of non-synonymous/synonymous mutation ratios (ω) for each site in spider and snake KTTs, as well as PBTI like body Kunitz proteins revealed high Darwinian selection pressure on the binding sites for Kv channels and serine proteases in snake, while only on the proteases in spider and none detected in body proteins, suggesting different rates and patterns of evolution among them. The results also revealed a series of key events in the history of spider KTT evolution, including the formation of a novel KTT family (named sub-Kuntiz-type toxins) derived from the ancestral native KTTs with the loss of the second disulfide bridge accompanied by several dramatic sequence modifications. Conclusions

Common molecular determinants of tarantula huwentoxin-IV inhibition of Na+ channel voltage sensors in domains II and IV.

PubMed

Xiao, Yucheng; Jackson, James O; Liang, Songping; Cummins, Theodore R

2011-08-05

The voltage sensors of domains II and IV of sodium channels are important determinants of activation and inactivation, respectively. Animal toxins that alter electrophysiological excitability of muscles and neurons often modify sodium channel activation by selectively interacting with domain II and inactivation by selectively interacting with domain IV. This suggests that there may be substantial differences between the toxin-binding sites in these two important domains. Here we explore the ability of the tarantula huwentoxin-IV (HWTX-IV) to inhibit the activity of the domain II and IV voltage sensors. HWTX-IV is specific for domain II, and we identify five residues in the S1-S2 (Glu-753) and S3-S4 (Glu-811, Leu-814, Asp-816, and Glu-818) regions of domain II that are crucial for inhibition of activation by HWTX-IV. These data indicate that a single residue in the S3-S4 linker (Glu-818 in hNav1.7) is crucial for allowing HWTX-IV to interact with the other key residues and trap the voltage sensor in the closed configuration. Mutagenesis analysis indicates that the five corresponding residues in domain IV are all critical for endowing HWTX-IV with the ability to inhibit fast inactivation. Our data suggest that the toxin-binding motif in domain II is conserved in domain IV. Increasing our understanding of the molecular determinants of toxin interactions with voltage-gated sodium channels may permit development of enhanced isoform-specific voltage-gating modifiers.

SVM-Based Prediction of Propeptide Cleavage Sites in Spider Toxins Identifies Toxin Innovation in an Australian Tarantula

PubMed Central

Wong, Emily S. W.; Hardy, Margaret C.; Wood, David; Bailey, Timothy; King, Glenn F.

2013-01-01

Spider neurotoxins are commonly used as pharmacological tools and are a popular source of novel compounds with therapeutic and agrochemical potential. Since venom peptides are inherently toxic, the host spider must employ strategies to avoid adverse effects prior to venom use. It is partly for this reason that most spider toxins encode a protective proregion that upon enzymatic cleavage is excised from the mature peptide. In order to identify the mature toxin sequence directly from toxin transcripts, without resorting to protein sequencing, the propeptide cleavage site in the toxin precursor must be predicted bioinformatically. We evaluated different machine learning strategies (support vector machines, hidden Markov model and decision tree) and developed an algorithm (SpiderP) for prediction of propeptide cleavage sites in spider toxins. Our strategy uses a support vector machine (SVM) framework that combines both local and global sequence information. Our method is superior or comparable to current tools for prediction of propeptide sequences in spider toxins. Evaluation of the SVM method on an independent test set of known toxin sequences yielded 96% sensitivity and 100% specificity. Furthermore, we sequenced five novel peptides (not used to train the final predictor) from the venom of the Australian tarantula Selenotypus plumipes to test the accuracy of the predictor and found 80% sensitivity and 99.6% 8-mer specificity. Finally, we used the predictor together with homology information to predict and characterize seven groups of novel toxins from the deeply sequenced venom gland transcriptome of S. plumipes, which revealed structural complexity and innovations in the evolution of the toxins. The precursor prediction tool (SpiderP) is freely available on ArachnoServer (http://www.arachnoserver.org/spiderP.html), a web portal to a comprehensive relational database of spider toxins. All training data, test data, and scripts used are available from the Spider

The VLT-FLAMES Tarantula Survey . XXIV. Stellar properties of the O-type giants and supergiants in 30 Doradus

NASA Astrophysics Data System (ADS)

Ramírez-Agudelo, O. H.; Sana, H.; de Koter, A.; Tramper, F.; Grin, N. J.; Schneider, F. R. N.; Langer, N.; Puls, J.; Markova, N.; Bestenlehner, J. M.; Castro, N.; Crowther, P. A.; Evans, C. J.; García, M.; Gräfener, G.; Herrero, A.; van Kempen, B.; Lennon, D. J.; Maíz Apellániz, J.; Najarro, F.; Sabín-Sanjulián, C.; Simón-Díaz, S.; Taylor, W. D.; Vink, J. S.

2017-04-01

Context. The Tarantula region in the Large Magellanic Cloud (LMC) contains the richest population of spatially resolved massive O-type stars known so far. This unmatched sample offers an opportunity to test models describing their main-sequence evolution and mass-loss properties. Aims: Using ground-based optical spectroscopy obtained in the framework of the VLT-FLAMES Tarantula Survey (VFTS), we aim to determine stellar, photospheric and wind properties of 72 presumably single O-type giants, bright giants and supergiants and to confront them with predictions of stellar evolution and of line-driven mass-loss theories. Methods: We apply an automated method for quantitative spectroscopic analysis of O stars combining the non-LTE stellar atmosphere model fastwind with the genetic fitting algorithm pikaia to determine the following stellar properties: effective temperature, surface gravity, mass-loss rate, helium abundance, and projected rotational velocity. The latter has been constrained without taking into account the contribution from macro-turbulent motions to the line broadening. Results: We present empirical effective temperature versus spectral subtype calibrations at LMC-metallicity for giants and supergiants. The calibration for giants shows a +1kK offset compared to similar Galactic calibrations; a shift of the same magnitude has been reported for dwarfs. The supergiant calibrations, though only based on a handful of stars, do not seem to indicate such an offset. The presence of a strong upturn at spectral type O3 and earlier can also not be confirmed by our data. In the spectroscopic and classical Hertzsprung-Russell diagrams, our sample O stars are found to occupy the region predicted to be the core hydrogen-burning phase by state-of-the-art models. For stars initially more massive than approximately 60 M⊙, the giant phase already appears relatively early on in the evolution; the supergiant phase develops later. Bright giants, however, are not

The Tarantula Massive Binary Monitoring. I. Observational campaign and OB-type spectroscopic binaries

NASA Astrophysics Data System (ADS)

Almeida, L. A.; Sana, H.; Taylor, W.; Barbá, R.; Bonanos, A. Z.; Crowther, P.; Damineli, A.; de Koter, A.; de Mink, S. E.; Evans, C. J.; Gieles, M.; Grin, N. J.; Hénault-Brunet, V.; Langer, N.; Lennon, D.; Lockwood, S.; Maíz Apellániz, J.; Moffat, A. F. J.; Neijssel, C.; Norman, C.; Ramírez-Agudelo, O. H.; Richardson, N. D.; Schootemeijer, A.; Shenar, T.; Soszyński, I.; Tramper, F.; Vink, J. S.

2017-02-01

Context. Massive binaries play a crucial role in the Universe. Knowing the distributions of their orbital parameters is important for a wide range of topics from stellar feedback to binary evolution channels and from the distribution of supernova types to gravitational wave progenitors, yet no direct measurements exist outside the Milky Way. Aims: The Tarantula Massive Binary Monitoring project was designed to help fill this gap by obtaining multi-epoch radial velocity (RV) monitoring of 102 massive binaries in the 30 Doradus region. Methods: In this paper we analyze 32 FLAMES/GIRAFFE observations of 93 O- and 7 B-type binaries. We performed a Fourier analysis and obtained orbital solutions for 82 systems: 51 single-lined (SB1) and 31 double-lined (SB2) spectroscopic binaries. Results: Overall, the binary fraction and orbital properties across the 30 Doradus region are found to be similar to existing Galactic samples. This indicates that within these domains environmental effects are of second order in shaping the properties of massive binary systems. A small difference is found in the distribution of orbital periods, which is slightly flatter (in log space) in 30 Doradus than in the Galaxy, although this may be compatible within error estimates and differences in the fitting methodology. Also, orbital periods in 30 Doradus can be as short as 1.1 d, somewhat shorter than seen in Galactic samples. Equal mass binaries (q> 0.95) in 30 Doradus are all found outside NGC 2070, the central association that surrounds R136a, the very young and massive cluster at 30 Doradus's core. Most of the differences, albeit small, are compatible with expectations from binary evolution. One outstanding exception, however, is the fact that earlier spectral types (O2-O7) tend to have shorter orbital periods than later spectral types (O9.2-O9.7). Conclusions: Our results point to a relative universality of the incidence rate of massive binaries and their orbital properties in the

The VLT-FLAMES Tarantula Survey. XVI. The optical and NIR extinction laws in 30 Doradus and the photometric determination of the effective temperatures of OB stars

NASA Astrophysics Data System (ADS)

Maíz Apellániz, J.; Evans, C. J.; Barbá, R. H.; Gräfener, G.; Bestenlehner, J. M.; Crowther, P. A.; García, M.; Herrero, A.; Sana, H.; Simón-Díaz, S.; Taylor, W. D.; van Loon, J. Th.; Vink, J. S.; Walborn, N. R.

2014-04-01

Context. The commonly used extinction laws of Cardelli et al. (1989, ApJ, 345, 245) have limitations that, among other issues, hamper the determination of the effective temperatures of O and early B stars from optical and near-infrared (NIR) photometry. Aims: We aim to develop a new family of extinction laws for 30 Doradus, check their general applicability within that region and elsewhere, and apply them to test the feasibility of using optical and NIR photometry to determine the effective temperature of OB stars. Methods: We use spectroscopy and NIR photometry from the VLT-FLAMES Tarantula Survey and optical photometry from HST/WFC3 of 30 Doradus and we analyze them with the software code CHORIZOS using different assumptions, such as the family of extinction laws. Results: We derive a new family of optical and NIR extinction laws for 30 Doradus and confirm its applicability to extinguished Galactic O-type systems. We conclude that by using the new extinction laws it is possible to measure the effective temperatures of OB stars with moderate uncertainties and only a small bias, at least up to E(4405-5495) ~ 1.5 mag. Appendices are available in electronic form at http://www.aanda.org

Taxonomic revision of the tarantula genus Aphonopelma Pocock, 1901 (Araneae, Mygalomorphae, Theraphosidae) within the United States

PubMed Central

Hamilton, Chris A.; Hendrixson, Brent E.; Bond, Jason E.

2016-01-01

Abstract This systematic study documents the taxonomy, diversity, and distribution of the tarantula spider genus Aphonopelma Pocock, 1901 within the United States. By employing phylogenomic, morphological, and geospatial data, we evaluated all 55 nominal species in the United States to examine the evolutionary history of Aphonopelma and the group’s taxonomy by implementing an integrative approach to species delimitation. Based on our analyses, we now recognize only 29 distinct species in the United States. We propose 33 new synonymies (Aphonopelma apacheum, Aphonopelma minchi, Aphonopelma rothi, Aphonopelma schmidti, Aphonopelma stahnkei = Aphonopelma chalcodes; Aphonopelma arnoldi = Aphonopelma armada; Aphonopelma behlei, Aphonopelma vogelae = Aphonopelma marxi; Aphonopelma breenei = Aphonopelma anax; Aphonopelma chambersi, Aphonopelma clarum, Aphonopelma cryptethum, Aphonopelma sandersoni, Aphonopelma sullivani = Aphonopelma eutylenum; Aphonopelma clarki, Aphonopelma coloradanum, Aphonopelma echinum, Aphonopelma gurleyi, Aphonopelma harlingenum, Aphonopelma odelli, Aphonopelma waconum, Aphonopelma wichitanum = Aphonopelma hentzi; Aphonopelma heterops = Aphonopelma moderatum; Aphonopelma jungi, Aphonopelma punzoi = Aphonopelma vorhiesi; Aphonopelma brunnius, Aphonopelma chamberlini, Aphonopelma iviei, Aphonopelma lithodomum, Aphonopelma smithi, Aphonopelma zionis = Aphonopelma iodius; Aphonopelma phanum, Aphonopelma reversum = Aphonopelma steindachneri), 14 new species (Aphonopelma atomicum sp. n., Aphonopelma catalina sp. n., Aphonopelma chiricahua sp. n., Aphonopelma icenoglei sp. n., Aphonopelma johnnycashi sp. n., Aphonopelma madera sp. n., Aphonopelma mareki sp. n., Aphonopelma moellendorfi sp. n., Aphonopelma parvum sp. n., Aphonopelma peloncillo sp. n., Aphonopelma prenticei sp. n., Aphonopelma saguaro sp. n., Aphonopelma superstitionense sp. n., and Aphonopelma xwalxwal sp. n.), and seven nomina dubia (Aphonopelma baergi, Aphonopelma cratium, Aphonopelma

HUBBLE TARANTULA TREASURY PROJECT. III. PHOTOMETRIC CATALOG AND RESULTING CONSTRAINTS ON THE PROGRESSION OF STAR FORMATION IN THE 30 DORADUS REGION

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sabbi, E.; Anderson, J.; Cignoni, M.

2016-01-15

We present and describe the astro-photometric catalog of more than 800,000 sources found in the Hubble Tarantula Treasury Project (HTTP). HTTP is a Hubble Space Telescope Treasury program designed to image the entire 30 Doradus region down to the sub-solar (∼0.5 M{sub ⊙}) mass regime using the Wide Field Camera 3 and the Advanced Camera for Surveys. We observed 30 Doradus in the near-ultraviolet (F275W, F336W), optical (F555W, F658N, F775W), and near-infrared (F110W, F160W) wavelengths. The stellar photometry was measured using point-spread function fitting across all bands simultaneously. The relative astrometric accuracy of the catalog is 0.4 mas. The astro-photometric catalog,more » results from artificial star experiments, and the mosaics for all the filters are available for download. Color–magnitude diagrams are presented showing the spatial distributions and ages of stars within 30 Dor as well as in the surrounding fields. HTTP provides the first rich and statistically significant sample of intermediate- and low-mass pre-main sequence candidates and allows us to trace how star formation has been developing through the region. The depth and high spatial resolution of our analysis highlight the dual role of stellar feedback in quenching and triggering star formation on the giant H ii region scale. Our results are consistent with stellar sub-clustering in a partially filled gaseous nebula that is offset toward our side of the Large Magellanic Cloud.« less

Venom Profiling of a Population of the Theraphosid Spider Phlogius crassipes Reveals Continuous Ontogenetic Changes from Juveniles through Adulthood

PubMed Central

Santana, Renan C.; Perez, David; Dobson, James; Panagides, Nadya; Raven, Robert J.; Nouwens, Amanda; Jones, Alun; King, Glenn F.; Fry, Bryan G.

2017-01-01

Theraphosid spiders (tarantulas) are venomous arthropods found in most tropical and subtropical regions of the world. Tarantula venoms are a complex cocktail of toxins with potential use as pharmacological tools, drugs and bioinsecticides. Although numerous toxins have been isolated from tarantula venoms, little research has been carried out on the venom of Australian tarantulas. We therefore investigated the venom profile of the Australian theraphosid spider Phlogius crassipes and examined whether there are ontogenetic changes in venom composition. Spiders were divided into four ontogenic groups according to cephalothorax length, then the venom composition of each group was examined using gel electrophoresis and mass spectrometry. We found that the venom of P. crassipes changes continuously during development and throughout adulthood. Our data highlight the need to investigate the venom of organisms over the course of their lives to uncover and understand the changing functions of venom and the full range of toxins expressed. This in turn should lead to a deeper understanding of the organism’s ecology and enhance the potential for biodiscovery. PMID:28346332

Venom Profiling of a Population of the Theraphosid Spider Phlogius crassipes Reveals Continuous Ontogenetic Changes from Juveniles through Adulthood.

PubMed

Santana, Renan C; Perez, David; Dobson, James; Panagides, Nadya; Raven, Robert J; Nouwens, Amanda; Jones, Alun; King, Glenn F; Fry, Bryan G

2017-03-25

Theraphosid spiders (tarantulas) are venomous arthropods found in most tropical and subtropical regions of the world. Tarantula venoms are a complex cocktail of toxins with potential use as pharmacological tools, drugs and bioinsecticides. Although numerous toxins have been isolated from tarantula venoms, little research has been carried out on the venom of Australian tarantulas. We therefore investigated the venom profile of the Australian theraphosid spider Phlogius crassipes and examined whether there are ontogenetic changes in venom composition. Spiders were divided into four ontogenic groups according to cephalothorax length, then the venom composition of each group was examined using gel electrophoresis and mass spectrometry. We found that the venom of P. crassipes changes continuously during development and throughout adulthood. Our data highlight the need to investigate the venom of organisms over the course of their lives to uncover and understand the changing functions of venom and the full range of toxins expressed. This in turn should lead to a deeper understanding of the organism's ecology and enhance the potential for biodiscovery.

The Multiplicity of Massive Stars: A High Angular Resolution Survey With The HST Fine Guidance Sensor

DTIC Science & Technology

2015-01-01

al. (2014), and of the Large Magellanic Cloud (LMC) Tarantula Nebula region by Sana et al. (2013b), demonstrate that the binary frequency may be »70...Monte-Carlo method to fit spectroscopic results for a large sample of O-type stars in the Tarantula Nebula region of the LMC, and they find a best fit

The VLT-FLAMES Tarantula Survey. XXVI. Properties of the O-dwarf population in 30 Doradus

NASA Astrophysics Data System (ADS)

Sabín-Sanjulián, C.; Simón-Díaz, S.; Herrero, A.; Puls, J.; Schneider, F. R. N.; Evans, C. J.; Garcia, M.; Najarro, F.; Brott, I.; Castro, N.; Crowther, P. A.; de Koter, A.; de Mink, S. E.; Gräfener, G.; Grin, N. J.; Holgado, G.; Langer, N.; Lennon, D. J.; Maíz Apellániz, J.; Ramírez-Agudelo, O. H.; Sana, H.; Taylor, W. D.; Vink, J. S.; Walborn, N. R.

2017-05-01

Context. The VLT-FLAMES Tarantula Survey has observed hundreds of O-type stars in the 30 Doradus region of the Large Magellanic Cloud (LMC). Aims: We study the properties of a statistically significant sample of O-type dwarfs in the same star-forming region and test the latest atmospheric and evolutionary models of the early main-sequence phase of massive stars. Methods: We performed quantitative spectroscopic analysis of 105 apparently single O-type dwarfs. To determine stellar and wind parameters, we used the iacob-gbat package, an automatic procedure based on a large grid of atmospheric models that are calculated with the fastwind code. This package was developed for the analysis of optical spectra of O-type stars. In addition to classical techniques, we applied the Bayesian bonnsai tool to estimate evolutionary masses. Results: We provide a new calibration of effective temperature vs. spectral type for O-type dwarfs in the LMC, based on our homogeneous analysis of the largest sample of such objects to date and including all spectral subtypes. Good agreement with previous results is found, although the sampling at the earliest subtypes could be improved. Rotation rates and helium abundances are studied in an evolutionary context. We find that most of the rapid rotators (v sin I > 300 km s-1) in our sample have masses below 25 M⊙ and intermediate rotation-corrected gravities (3.9 < log gc < 4.1). Such rapid rotators are scarce at higher gravities (I.e. younger ages) and absent at lower gravities (larger ages). This is not expected from theoretical evolutionary models, and does not appear to be due to a selection bias in our sample. We compare the estimated evolutionary and spectroscopic masses, finding a trend that the former is higher for masses below 20 M⊙. This can be explained as a consequence of limiting our sample to the O-type stars, and we see no compelling evidence for a systematic mass discrepancy. For most of the stars in the sample we were unable

[Tarantism in Spain in the eighteen century: latrodectism and suggestion].

PubMed

Corral-Corral, I; Corral-Corral, C

2016-10-16

Tarantism is the disease caused by the bite of the tarantula, in which the music tarantella triggers an involuntary dance. It is known in Italy since the sixteenth century. To analyze the tarantism reported in Spain at the end of the eighteenth century, with special attention to its neurological aspects, and to propose its medical and psychopathological explanation. An epidemic of people affected by the tarantula bite occurred in Spain in 1782. Spanish doctors described appropriately the clinical effects, identical to those produced by the bite of the spider black widow (Latrodectus tredecimguttatus), which was at that time identified as a tarantula. The cases reported by Francisco Xavier Cid cured with the involuntary dance triggered by the tarantella, as was described in Italy since the sixteenth century. Our interpretation is that this curative effect of dance in Spain was induced by suggestion. In Spanish patients there were no behavioral disturbances, periodic recurrences or collective involvement as those reported by Italian authors, which suggest an hysterical phenomenon, probably a continuation of the dancing mania of the Middle Age. Tarantism reported in Spain in the eighteenth century includes two different phenomena: the systemic symptoms produced by the tarantula bite, which is actually latrodectism, and the curative effect of the tarantella, explained by suggestion. The psychiatric disturbances, with a hysterical nature, falsely associated to the tarantula bite, observed in Italy, were not present among the Spanish cases of tarantism in the eighteenth century.

The VLT-FLAMES Tarantula Survey. XIX. B-type supergiants: Atmospheric parameters and nitrogen abundances to investigate the role of binarity and the width of the main sequence

NASA Astrophysics Data System (ADS)

McEvoy, C. M.; Dufton, P. L.; Evans, C. J.; Kalari, V. M.; Markova, N.; Simón-Díaz, S.; Vink, J. S.; Walborn, N. R.; Crowther, P. A.; de Koter, A.; de Mink, S. E.; Dunstall, P. R.; Hénault-Brunet, V.; Herrero, A.; Langer, N.; Lennon, D. J.; Maíz Apellániz, J.; Najarro, F.; Puls, J.; Sana, H.; Schneider, F. R. N.; Taylor, W. D.

2015-03-01

Context. Model atmosphere analyses have been previously undertaken for both Galactic and extragalactic B-type supergiants. By contrast, little attention has been given to a comparison of the properties of single supergiants and those that are members of multiple systems. Aims: Atmospheric parameters and nitrogen abundances have been estimated for all the B-type supergiants identified in the VLT-FLAMES Tarantula survey. These include both single targets and binary candidates. The results have been analysed to investigate the role of binarity in the evolutionary history of supergiants. Methods: tlusty non-local thermodynamic equilibrium (LTE) model atmosphere calculations have been used to determine atmospheric parameters and nitrogen abundances for 34 single and 18 binary supergiants. Effective temperatures were deduced using the silicon balance technique, complemented by the helium ionisation in the hotter spectra. Surface gravities were estimated using Balmer line profiles and microturbulent velocities deduced using the silicon spectrum. Nitrogen abundances or upper limits were estimated from the N ii spectrum. The effects of a flux contribution from an unseen secondary were considered for the binary sample. Results: We present the first systematic study of the incidence of binarity for a sample of B-type supergiants across the theoretical terminal age main sequence (TAMS). To account for the distribution of effective temperatures of the B-type supergiants it may be necessary to extend the TAMS to lower temperatures. This is also consistent with the derived distribution of mass discrepancies, projected rotational velocities and nitrogen abundances, provided that stars cooler than this temperature are post-red supergiant objects. For all the supergiants in the Tarantula and in a previous FLAMES survey, the majority have small projected rotational velocities. The distribution peaks at about 50 km s-1 with 65% in the range 30 km s-1 ≤ vesini ≤ 60 km s-1. About

The VLT-FLAMES Tarantula Survey. XVII. Physical and wind properties of massive stars at the top of the main sequence

NASA Astrophysics Data System (ADS)

Bestenlehner, J. M.; Gräfener, G.; Vink, J. S.; Najarro, F.; de Koter, A.; Sana, H.; Evans, C. J.; Crowther, P. A.; Hénault-Brunet, V.; Herrero, A.; Langer, N.; Schneider, F. R. N.; Simón-Díaz, S.; Taylor, W. D.; Walborn, N. R.

2014-10-01

The evolution and fate of very massive stars (VMS) is tightly connected to their mass-loss properties. Their initial and final masses differ significantly as a result of mass loss. VMS have strong stellar winds and extremely high ionising fluxes, which are thought to be critical sources of both mechanical and radiative feedback in giant H ii regions. However, how VMS mass-loss properties change during stellar evolution is poorly understood. In the framework of the VLT-Flames Tarantula Survey (VFTS), we explore the mass-loss transition region from optically thin O star winds to denser WNh Wolf-Rayet star winds, thereby testing theoretical predictions. To this purpose we select 62 O, Of, Of/WN, and WNh stars, an unprecedented sample of stars with the highest masses and luminosities known. We perform a spectral analysis of optical VFTS as well as near-infrared VLT/SINFONI data using the non-LTE radiative transfer code CMFGEN to obtain both stellar and wind parameters. For the first time, we observationally resolve the transition between optically thin O star winds and optically thick hydrogen-rich WNh Wolf-Rayet winds. Our results suggest the existence of a "kink" between both mass-loss regimes, in agreement with recent Monte Carlo simulations. For the optically thick regime, we confirm the steep dependence on the classical Eddington factor Γe from previous theoretical and observational studies. The transition occurs on the main sequence near a luminosity of 106.1L⊙, or a mass of 80 ... 90 M⊙. Above this limit, we find that - even when accounting for moderate wind clumping (with fv = 0.1) - wind mass-loss rates are enhanced with respect to standard prescriptions currently adopted in stellar evolution calculations. We also show that this results in substantial helium surface enrichment. Finally, based on our spectroscopic analyses, we are able to provide the most accurate ionising fluxes for VMS known to date, confirming the pivotal role of VMS in ionising and

Structure and Orientation of a Voltage-Sensor Toxin in Lipid Membranes

PubMed Central

Jung, Hyun Ho; Jung, Hoi Jong; Milescu, Mirela; Lee, Chul Won; Lee, Seungkyu; Lee, Ju Yeon; Eu, Young-Jae; Kim, Ha Hyung; Swartz, Kenton J.; Kim, Jae Il

2010-01-01

Abstract Amphipathic protein toxins from tarantula venom inhibit voltage-activated potassium (Kv) channels by binding to a critical helix-turn-helix motif termed the voltage sensor paddle. Although these toxins partition into membranes to bind the paddle motif, their structure and orientation within the membrane are unknown. We investigated the interaction of a tarantula toxin named SGTx with membranes using both fluorescence and NMR spectroscopy. Depth-dependent fluorescence-quenching experiments with brominated lipids suggest that Trp30 in SGTx is positioned ∼9 Å from the center of the bilayer. NMR spectra reveal that the inhibitor cystine knot structure of the toxin does not radically change upon membrane partitioning. Transferred cross-saturation NMR experiments indicate that the toxin's hydrophobic protrusion contacts the hydrophobic core of the membrane, whereas most surrounding polar residues remain at interfacial regions of the bilayer. The inferred orientation of the toxin reveals a twofold symmetry in the arrangement of basic and hydrophobic residues, a feature that is conserved among tarantula toxins. These results have important implications for regions of the toxin involved in recognizing membranes and voltage-sensor paddles, and for the mechanisms by which tarantula toxins alter the activity of different types of ion channels. PMID:20643084

Subjective anxiety and behavioral avoidance: Gender, gender role, and perceived confirmability of self-report.

PubMed

McLean, Carmen P; Hope, Debra A

2010-06-01

Commonly reported gender effects for differential vulnerability for anxiety may relate to gender socialization processes. The present study examined the relationship between gender role and fear under experimental conditions designed to elicit accurate fear reporting. Undergraduate students (N=119) completed several self-report measures and a behavioral avoidance task (BAT) with a tarantula while wearing a heart rate monitor. Gender roles were operationalized as instrumentality and expressiveness, as measured by the Personal Attributes Questionnaire (Spence, Helmreich, & Stapp, 1975). As expected, women reported greater subjective anxiety and were more avoidant of the tarantula than men. Regardless of gender, low levels of instrumentality were associated with greater avoidance of the tarantula. The hypothesis that men underreport fear compared to women and that gender role differences underlie this reporting bias was not supported. In spite of a ceiling effect on the BAT, results of this study confirm the relevance of gender role in understanding gender effects in fear and anxiety. Copyright 2010 Elsevier Ltd. All rights reserved.

The tarantula toxins ProTx-II and huwentoxin-IV differentially interact with human Nav1.7 voltage sensors to inhibit channel activation and inactivation.

PubMed

Xiao, Yucheng; Blumenthal, Kenneth; Jackson, James O; Liang, Songping; Cummins, Theodore R

2010-12-01

The voltage-gated sodium channel Na(v)1.7 plays a crucial role in pain, and drugs that inhibit hNa(v)1.7 may have tremendous therapeutic potential. ProTx-II and huwentoxin-IV (HWTX-IV), cystine knot peptides from tarantula venoms, preferentially block hNa(v)1.7. Understanding the interactions of these toxins with sodium channels could aid the development of novel pain therapeutics. Whereas both ProTx-II and HWTX-IV have been proposed to preferentially block hNa(v)1.7 activation by trapping the domain II voltage-sensor in the resting configuration, we show that specific residues in the voltage-sensor paddle of domain II play substantially different roles in determining the affinities of these toxins to hNa(v)1.7. The mutation E818C increases ProTx-II's and HWTX-IV's IC(50) for block of hNa(v)1.7 currents by 4- and 400-fold, respectively. In contrast, the mutation F813G decreases ProTx-II affinity by 9-fold but has no effect on HWTX-IV affinity. It is noteworthy that we also show that ProTx-II, but not HWTX-IV, preferentially interacts with hNa(v)1.7 to impede fast inactivation by trapping the domain IV voltage-sensor in the resting configuration. Mutations E1589Q and T1590K in domain IV each decreased ProTx-II's IC(50) for impairment of fast inactivation by ~6-fold. In contrast mutations D1586A and F1592A in domain-IV increased ProTx-II's IC(50) for impairment of fast inactivation by ~4-fold. Our results show that whereas ProTx-II and HWTX-IV binding determinants on domain-II may overlap, domain II plays a much more crucial role for HWTX-IV, and contrary to what has been proposed to be a guiding principle of sodium channel pharmacology, molecules do not have to exclusively target the domain IV voltage-sensor to influence sodium channel inactivation.

Sexual cannibalism: high incidence in a natural population with benefits to females.

PubMed

Rabaneda-Bueno, Rubén; Rodríguez-Gironés, Miguel A; Aguado-de-la-Paz, Sara; Fernández-Montraveta, Carmen; De Mas, Eva; Wise, David H; Moya-Laraño, Jordi

2008-01-01

Sexual cannibalism may be a form of extreme sexual conflict in which females benefit more from feeding on males than mating with them, and males avoid aggressive, cannibalistic females in order to increase net fitness. A thorough understanding of the adaptive significance of sexual cannibalism is hindered by our ignorance of its prevalence in nature. Furthermore, there are serious doubts about the food value of males, probably because most studies that attempt to document benefits of sexual cannibalism to the female have been conducted in the laboratory with non-natural alternative prey. Thus, to understand more fully the ecology and evolution of sexual cannibalism, field experiments are needed to document the prevalence of sexual cannibalism and its benefits to females. We conducted field experiments with the Mediterranean tarantula (Lycosa tarantula), a burrowing wolf spider, to address these issues. At natural rates of encounter with males, approximately a third of L. tarantula females cannibalized the male. The rate of sexual cannibalism increased with male availability, and females were more likely to kill and consume an approaching male if they had previously mated with another male. We show that females benefit from feeding on a male by breeding earlier, producing 30% more offspring per egg sac, and producing progeny of higher body condition. Offspring of sexually cannibalistic females dispersed earlier and were larger later in the season than spiderlings of non-cannibalistic females. In nature a substantial fraction of female L. tarantula kill and consume approaching males instead of mating with them. This behaviour is more likely to occur if the female has mated previously. Cannibalistic females have higher rates of reproduction, and produce higher-quality offspring, than non-cannibalistic females. Our findings further suggest that female L. tarantula are nutrient-limited in nature and that males are high-quality prey. The results of these field

Sexual Cannibalism: High Incidence in a Natural Population with Benefits to Females

PubMed Central

Rabaneda-Bueno, Rubén; Rodríguez-Gironés, Miguel Á.; Aguado-de-la-Paz, Sara; Fernández-Montraveta, Carmen; De Mas, Eva; Wise, David H.; Moya-Laraño, Jordi

2008-01-01

Background Sexual cannibalism may be a form of extreme sexual conflict in which females benefit more from feeding on males than mating with them, and males avoid aggressive, cannibalistic females in order to increase net fitness. A thorough understanding of the adaptive significance of sexual cannibalism is hindered by our ignorance of its prevalence in nature. Furthermore, there are serious doubts about the food value of males, probably because most studies that attempt to document benefits of sexual cannibalism to the female have been conducted in the laboratory with non-natural alternative prey. Thus, to understand more fully the ecology and evolution of sexual cannibalism, field experiments are needed to document the prevalence of sexual cannibalism and its benefits to females. Methodology/Principal Findings We conducted field experiments with the Mediterranean tarantula (Lycosa tarantula), a burrowing wolf spider, to address these issues. At natural rates of encounter with males, approximately a third of L. tarantula females cannibalized the male. The rate of sexual cannibalism increased with male availability, and females were more likely to kill and consume an approaching male if they had previously mated with another male. We show that females benefit from feeding on a male by breeding earlier, producing 30% more offspring per egg sac, and producing progeny of higher body condition. Offspring of sexually cannibalistic females dispersed earlier and were larger later in the season than spiderlings of non-cannibalistic females. Conclusions/Significance In nature a substantial fraction of female L. tarantula kill and consume approaching males instead of mating with them. This behaviour is more likely to occur if the female has mated previously. Cannibalistic females have higher rates of reproduction, and produce higher-quality offspring, than non-cannibalistic females. Our findings further suggest that female L. tarantula are nutrient-limited in nature and that

The stellar content of 30 Doradus

NASA Technical Reports Server (NTRS)

Walborn, N. R.

1984-01-01

The components of the supergiant H II region Tarantula are surveyed, noting that 30 Doradus is really only the most active section of the Large Magellanic Cloud. The region contains at least 40 WR stars and numerous non-H II region late spectral type supergiants. Most of the stars are centrally located and presumably feed on the nebulosity. The closeness of the population will require fine spectroscopic scans of all the members to achieve accurate typing. Although the population is mixed, the ionizing radiation emitted by the region is consistent with its classification as part of the H II region. Finally, the brightest objects within Tarantula are suspected of being multiple systems.

[Dancing manias. Between culture and medicine].

PubMed

Prochwicz, Katarzyna; Sobczyk, Artur

2011-01-01

Dancing mania is a clinical and cultural phenomenon which occurred in Western Europe between 13th and 18th centuries. The term dancing mania is derived from the Greek words choros, a dance, and mania, a madness. An Italian variant was known as tarantism as victims were believed to have been bitten by tarantula spider. Although symptoms of dancing manias were well documented in contemporary writings the exact aetiology of dancing plaques is still unclear. Several causes for dancing mania have been postulated: demonic possession, the bite of tarantula, ergot poisoning, epilepsy, mass hysterias, exotics religious cults. The article contains a review of hypothesis of epidemic dances included both medical and psychological factors.

A Mating of Tarantulas

ERIC Educational Resources Information Center

Lewis, Robert L.

1978-01-01

The role of the trustee in interinstitutional cooperation is discussed. Benefits include: plant facilities sharing, joint credit unions, library exchange, joint health care facilities, joint counseling, joint apprenticeship programs, cross registration, faculty exchange joint appointments, etc. (Author/LBH)

Viewing behaviour of spider phobics and non-phobics in the presence of threat and safety stimuli.

PubMed

Lange, W G T; Tierney, K J; Reinhardt-Rutland, A H; Vivekananda-Schmidt, P

2004-09-01

To determine whether spider phobics and spider non-phobics differ in attending to threat and safety stimuli in close proximity, or spatially separated. A sample group of 16 spider-phobic and 16 spider-non-phobic volunteers were drawn from a student population on the basis of their scores on Watts and Sharrock's Spider Phobia Questionnaire. Attention was assessed by way of participants' viewing behaviour using an ASL 501 head-mounted eye-tracking system. In a control condition, participants viewed a neutral stimulus (a TV video) in the absence of the threat stimulus. In two experimental conditions, the threat stimulus (a live Chilean rose tarantula) was introduced (a) immediately beside the safety stimulus (the only exit of the experimental room), or (b) away from the safety stimulus. In the experimental conditions, phobics reduced their viewing of the TV and increased their viewing of the tarantula and of the exit; the biggest changes occurred when tarantula and exit were together. Phobics also made more eye motions across the experimental room. Our results are consistent with previous research employing reaction time to a neutral stimulus as dependent measure. In addition, our results suggest that phobics scan the environment as part of safety behaviour. We suggest that exposure treatments to reduce spider phobia may be facilitated by encouraging patients to stop environmental scanning. Copyright 2004 The British Psychological Society

The VLT-FLAMES Tarantula Survey. XI. A census of the hot luminous stars and their feedback in 30 Doradus

NASA Astrophysics Data System (ADS)

Doran, E. I.; Crowther, P. A.; de Koter, A.; Evans, C. J.; McEvoy, C.; Walborn, N. R.; Bastian, N.; Bestenlehner, J. M.; Gräfener, G.; Herrero, A.; Köhler, K.; Maíz Apellániz, J.; Najarro, F.; Puls, J.; Sana, H.; Schneider, F. R. N.; Taylor, W. D.; van Loon, J. Th.; Vink, J. S.

2013-10-01

Context. The VLT-FLAMES Tarantula Survey has an extensive view of the copious number of massive stars in the 30 Doradus (30 Dor) star forming region of the Large Magellanic Cloud. These stars play a crucial role in our understanding of the stellar feedback in more distant, unresolved star forming regions. Aims: The first comprehensive census of hot luminous stars in 30 Dor is compiled within a 10 arcmin (150 pc) radius of its central cluster, R136. We investigate the stellar content and spectroscopic completeness of the early type stars. Estimates were made for both the integrated ionising luminosity and stellar wind luminosity. These values were used to re-assess the star formation rate (SFR) of the region and determine the ionising photon escape fraction. Methods: Stars were selected photometrically and combined with the latest spectral classifications. Spectral types were estimated for stars lacking spectroscopy and corrections were made for binary systems, where possible. Stellar calibrations were applied to obtain their physical parameters and wind properties. Their integrated properties were then compared to global observations from ultraviolet (UV) to far-infrared (FIR) imaging as well as the population synthesis code, Starburst99. Results: Our census identified 1145 candidate hot luminous stars within 150 pc of R136 of which >700 were considered to be genuine early type stars and contribute to feedback. We assess the survey to be spectroscopically complete to 85% in the outer regions (>5 pc) but only 35% complete in the region of the R136 cluster, giving a total of 500 hot luminous stars in the census which had spectroscopy. Only 31 were found to be Wolf-Rayet (W-R) or Of/WN stars, but their contribution to the integrated ionising luminosity and wind luminosity was ~40% and ~50%, respectively. Similarly, stars with Minit > 100 M⊙ (mostly H-rich WN stars) also showed high contributions to the global feedback, ~25% in both cases. Such massive stars are not

Anti-inflammatory and protective investigations on the effects of Theranekron® "an alcoholic extract of the Tarantula cubensis" on wound healing of peritoneal in the rat: an in vivo comparative study.

PubMed

Adib-Hashemi, Farajollah; Farahmand, Farshad; Hesari, Shamim Fattah; Rezakhaniha, Bijan; Fallah, Ehsan; Fayyaz, Amir Farshid; Dadpay, Masoomeh

2015-04-02

The present study sought to investigate the effects of Tarantula cubensis extract (TC; Theranekron®) on the histopathological scores of peritoneal wound healing after laparotomy in the rats. This study was designed to investigate the effects of Theranekron on the peritoneal wound healing after wound creation, on days9, 14, 19, 24 and 29 post-injury in rats. Twenty-four mature Wister-albino male rats were randomly divided into two groups. In the experimental group, TC was repeatedly injected subcutaneously (SC) over the lesion 9, 14,19 and 24 days after laparotomy, whereas the control group received only normal saline by subcutaneous injection and then the animal groups were euthanized9, 14, 19, 24, and 29 days after wounding respectively by intravenous injections of pentobarbital (50 mg/kg). Finally, assessment of the peritoneal wound healing between the groups was carried out by histopathologic data and statistical tests as Mann-Whitney U, Wilcoxon W and Z RESULTS: Histopathological examination indicated significant improvement in angiogenesis, re-epithelialization and less inflammatory response in comparison to control and also, revealed matured, compact and parallel deposition of collagen fibrils on day 29. So, at long term, treatment reduced the inflammation and increased the quality and rate of wound re- epithelialization compared to controls(P < 0.05). Furthermore, excluding the control group, rats exhibited the most pronounced effect on wound closure, with the statistically significant improvement in wound healing being seen at post-operative day 29. Moreover, collagen content on days 24 and 29 in the test group was found to be higher than in the healthy group. To warp up, treated groups had a significant increase in peritoneal wound healing area compared to the control group on all days (P < 0.05). Our results suggested that Theranekron have delivered a novel therapeutic route for wound treatment in clinical practice. The virtual slide(s) for

T-ReX Spies the Stars of 30 Doradus

NASA Astrophysics Data System (ADS)

Broos, Patrick; Townsley, Leisa K.; Pollock, Andrew; Crowther, Paul

2017-08-01

30 Doradus (the Tarantula Nebula) is the Local Group's most massive young star-forming complex. At its heart is R136, the most massive resolved stellar cluster; R136 contains, in turn, the most massive stars known. The Chandra X-ray Observatory has recently observed 30 Dor for the 2-megasecond X-ray Visionary Project ``The Tarantula -- Revealed by X-rays'' (T-ReX). This deep observation exploits Chandra's fine spatial resolution to study the full complement of massive stars and the brightest pre-main sequence stars that trace 25 Myrs of star formation in this incomparable nearby starburst. Here we give preliminary results from the ongoing analyses of the data, focusing on the massive stars. While many remain undetected even in this deep ACIS-I observation, a few show dramatic X-ray lightcurves and/or high luminosities befitting this amazing starburst cluster.

An Annotated List of Tick (Acari: Ixodida) Common Names Authored by Harry Hoogstraal (1917-1986)

DTIC Science & Technology

2011-01-01

to the comparatively recent rules promulgated by the American Arachnological Society Committee on Common Names of Arachnids (2003), most of... Arachnological Society Committee on Common Names of Arachnids (2003) Common Names of Arachnids, Fifth Edition. American Tarantula Society, accessible online

Hubble Unveils Colorful and Turbulent Star-Birth Region on 100,000th Orbit Milestone

NASA Image and Video Library

2008-08-10

NASA Hubble peers into a small portion of the nebula near the star cluster NGC 2074. The region is a firestorm of raw stellar creation, perhaps triggered by a nearby supernova explosion. It lies about 170,000 light-years away near the Tarantula

Proceedings of the Annual Meeting of the Association for Education in Journalism and Mass Communication (83rd, Phoenix, Arizona, August 9-12, 2000). Cultural and Critical Studies Division.

ERIC Educational Resources Information Center

Association for Education in Journalism and Mass Communication.

The Cultural and Critical Studies Division section of the proceedings contains the following 11 papers: "'Grimm' News Indeed--'Madstones,' Clever Toads, and Killer Tarantulas: Fairy-Tale Briefs in Wild West Newspapers" (Paulette Kilmer); "The First Amendment and the Doctrine of Corporate Personhood: Collapsing the Press-Corporation…

Taxonomic revision and cladistic analysis of Avicularia Lamarck, 1818 (Araneae, Theraphosidae, Aviculariinae) with description of three new aviculariine genera

PubMed Central

Fukushima, Caroline Sayuri; Bertani, Rogério

2017-01-01

Abstract The genus Avicularia Lamarck, 1818 is revised and all species are rediagnosed. The type species, described as Aranea avicularia Linnaeus, 1758, is the oldest mygalomorph species described and its taxonomic history is extensive and confusing. Cladistic analyses using both equal and implied weights were carried out with a matrix of 46 taxa from seven theraphosid subfamilies, and 71 morphological and ecological characters. The optimal cladogram found with Piwe and concavity = 6 suggests Avicularia and Aviculariinae are monophyletic. Subfamily Aviculariinae includes Avicularia Lamarck, 1818, Typhochlaena C. L. Koch, 1850, Tapinauchenius Ausserer, 1871, Stromatopelma Karsch, 1881, Ephebopus Simon, 1892, Psalmopoeus Pocock, 1895, Heteroscodra Pocock, 1899, Iridopelma Pocock, 1901, Pachistopelma Pocock, 1901, Ybyrapora gen. n., Caribena gen. n., and Antillena gen. n. The clade is supported by well-developed scopulae on tarsi and metatarsi, greatly extended laterally. Avicularia synapomorphies are juveniles bearing black tarsi contrasting with other lighter articles; spermathecae with an accentuated outwards curvature medially, and male palpal bulb with embolus medial portion and tegulum’s margin form an acute angle in retrolateral view. Avicularia is composed of twelve species, including three new species: Avicularia avicularia (Linnaeus, 1818), Avicularia glauca Simon, 1891, Avicularia variegata (F. O. Pickard-Cambridge, 1896) stat. n., Avicularia minatrix Pocock, 1903, Avicularia taunayi (Mello-Leitão, 1920), Avicularia juruensis Mello-Leitão, 1923, Avicularia rufa Schiapelli & Gerschman, 1945, Avicularia purpurea Kirk, 1990, Avicularia hirschii Bullmer et al. 2006, Avicularia merianae sp. n., Avicularia lynnae sp. n., and Avicularia caei sp. n.. Avicularia species are distributed throughout Mexico, Costa Rica, Panama, Trinidad and Tobago, Venezuela, Guyana, Suriname, French Guiana, Colombia, Ecuador, Peru, Bolivia, and Brazil. Three new genera are erected

Change Agent Survival Guide

ERIC Educational Resources Information Center

Dunbar, Folwell L.

2011-01-01

Consulting is a rough racket. Only a tarantula hair above IRS agents, meter maids and used car sales people, the profession is a prickly burr for slings and arrows. Throw in education, focus on dysfunctional schools and call oneself a "change agent," and this bad rap all but disappears. Unfortunately, though, consulting/coaching/mentoring in…

Gender, gender roles, and anxiety: perceived confirmability of self report, behavioral avoidance, and physiological reactivity.

PubMed

Stoyanova, Milena; Hope, Debra A

2012-01-01

Despite the well-documented gender effect in anxiety, less is known about contributing factors to women's greater risk for anxiety and fears. The present study examined the relationship between gender, gender role orientation (i.e., expressivity/instrumentality) and fear of harmless insects (tarantula), using a multimodal approach of self-report measures, a Behavioral Approach Test (BAT), and physiological reactivity. Participants (144 college students; 67 women, 77 men) completed a questionnaire packet and then were instructed to approach a tarantula. We were unable to replicate Pierce and Kirkpatrick's (1992) findings that men underreport anxiety. Consistent with the literature, women in the study experienced greater anxiety and avoidance compared to men. However, men and women did not differ on physiological reactivity during the first 2 min of the BAT. The concordance across avoidance, anxiety and heart rate reactivity differed by gender, suggesting that men and women have different experiences when faced with a fearful object. Furthermore, instrumentality (masculinity) was negatively related to anticipatory anxiety for women but not for men. Copyright © 2011 Elsevier Ltd. All rights reserved.

Cosmic Spider is Good Mother

NASA Astrophysics Data System (ADS)

2006-04-01

Hanging above the Large Magellanic Cloud (LMC) - one of our closest galaxies - in what some describe as a frightening sight, the Tarantula nebula is worth looking at in detail. Also designated 30 Doradus or NGC 2070, the nebula owes its name to the arrangement of its brightest patches of nebulosity that somewhat resemble the legs of a spider. This name, of the biggest spiders on Earth, is also very fitting in view of the gigantic proportions of the celestial nebula - it measures nearly 1,000 light years across! ESO PR Photo 11/06 ESO PR Photo 13b/06 Tarantula's Central Cluster, R136 The Tarantula nebula is the largest emission nebula in the sky and also one of the largest known star-forming regions in all the Milky Way's neighbouring galaxies. Located about 170,000 light-years away, in the southern constellation Dorado (The Swordfish), it can be seen with the unaided eye. As shown in this image obtained with the FORS1 multi-mode instrument on ESO's Very Large Telescope, its structure is fascinatingly complex, with a large number of bright arcs and apparently dark areas in between. Inside the giant emission nebula lies a cluster of young, massive and hot stars, denoted R 136, whose intense radiation and strong winds make the nebula glow, shaping it into the form of a giant arachnid. The cluster is about 2 to 3 million years old, that is, almost from 'yesterday' in the 13.7 billion year history of the Universe. Several of the brighter members in the immediate surroundings of the dense cluster are among the most massive stars known, with masses well above 50 times the mass of our Sun. The cluster itself contains more than 200 massive stars. ESO PR Photo 11/06 ESO PR Photo 13c/06 The Stellar Cluster Hodge 301 In the upper right of the image, another cluster of bright, massive stars is seen. Known to astronomers as Hodge 301, it is about 20 million years old, or about 10 times older than R136. The more massive stars of Hodge 301 have therefore already exploded as

Dissecting Diffuse X-ray Emission in 30 Doradus with T-ReX

NASA Astrophysics Data System (ADS)

Townsley, Leisa K.; Broos, Patrick

2017-08-01

30 Doradus (the Tarantula Nebula) offers us a microscope on starburst astrophysics, having endured 25 Myrs of the birth and death of the most massive stars known. Across 30 Dor's 250-pc extent, stellar winds and supernovae have carved its ISM into an amazing display of arcs, pillars, and bubbles. For over 40 years, we have also known that 30 Dor is a bright X-ray emitter, so its familiar stars and cold ISM structures suffer irradiation by multi-million-degree plasmas. The 2-Ms Chandra X-ray Visionary Project ``The Tarantula -- Revealed by X-rays'' (T-ReX) exploits Chandra's fine spatial resolution and the ACIS-I field of view to study ISM interfaces on 1--10 pc scales across the entire 30 Dor complex. Here we give preliminary results from ongoing analyses of these data, focusing on the diffuse X-ray emission. Massive star winds and cavity supernovae over the millenia have contributed to a broad mix of X-ray-emitting plasmas and absorbing columns, showing that 30 Dor's hot ISM is just as complex and confusing as that seen at colder temperatures.

Air Gaps, Size Effect, and Corner-Turning in Ambient LX-17

DOE Office of Scientific and Technical Information (OSTI.GOV)

Souers, P C; Hernandez, A; Cabacungan, C

2008-02-05

Various ambient measurements are presented for LX-17. The size (diameter) effect has been measured with copper and Lucite confinement, where the failure radii are 4.0 and 6.5 mm, respectively. The air well corner-turn has been measured with an LX-07 booster, and the dead-zone results are comparable to the previous TATB-boosted work. Four double cylinders have been fired, and dead zones appear in all cases. The steel-backed samples are faster than the Lucite-backed samples by 0.6 {micro}s. Bare LX-07 and LX-17 of 12.7 mm-radius were fired with air gaps. Long acceptor regions were used to truly determine if detonation occurred ormore » not. The LX-07 crossed at 10 mm with a slight time delay. Steady state LX-17 crossed at 3.5 mm gap but failed to cross at 4.0 mm. LX-17 with a 12.7 mm run after the booster crossed a 1.5 mm gap but failed to cross 2.5 mm. Timing delays were measured where the detonation crossed the gaps. The Tarantula model is introduced as embedded in 0 reactive flow JWL++ and Linked Cheetah V4, mostly at 4 zones/mm. Tarantula has four pressure regions: off, initiation, failure and detonation. The physical basis of the input parameters is considered.« less

Are amphitheater headed canyons indicative of a particular formative process?

NASA Astrophysics Data System (ADS)

Ryan, A. J.; Whipple, K. X.; Johnson, J. P.

2012-12-01

Tributary canyons with amphitheater-shaped heads have previously been interpreted as evidence for groundwater seepage erosion, particularly in environments where fluvial processes are assumed to be negligible. However, some have questioned whether this canyon morphology is truly diagnostic of a particular formative process. We seek to determine the relative roles of fluvial and groundwater-related processes and the strength of stratigraphic control on the Colorado Plateau through a combination of fieldwork and GIS analysis. Amphitheater valleys may have overhanging or steep-sided headwalls with a semicircular plan-view pattern. It is reasonable to assume that this form is a result of focused erosion at the base of the headwall (i.e. sapping). Two frequently cited agents may lead to undermining: plunge-pool scour at the base of waterfalls and seepage induced weathering and erosion where the groundwater table intersects the land surface. Both processes are enhanced where weaker, less permeable layers underlie stronger cap rock. We conducted preliminary fieldwork in two locations on the Colorado Plateau, where there are many classic examples of amphitheater headed canyons. The Escalante River landscape is highly variable with a range of canyon and valley-head forms, many of which cut through the thick Navajo Sandstone into the underlying shale and sand of the Kayenta Formation. Northeast of Escalante National Monument, at the base of the Henry Mountains, is Tarantula Mesa. The canyons there are also considerably variable, with nearly all containing at least one abrupt amphitheater knickpoint at the valley head or farther downstream. Our observations are presented here with an analysis of the canyon profiles, surrounding topography, and potential structural controls. We have found that nearly all amphitheaters in both locales show signs of groundwater seepage weathering and plausibly seepage erosion. However, many also contain plunge pools and evidence of substantial

Portrait of a Dramatic Stellar Crib

NASA Astrophysics Data System (ADS)

2006-12-01

A new, stunning image of the cosmic spider, the Tarantula Nebula and its surroundings, finally pays tribute to this amazing, vast and intricately sculpted web of stars and gas. The newly released image, made with ESO's Wide Field Imager on the 2.2-m ESO/MPG Telescope at La Silla, covers 1 square degree on the sky and could therefore contain four times the full Moon. ESO PR Photo 50a/06 ESO PR Photo 50a/06 The Tarantula Nebula (WFI/2.2m) Known as the Tarantula Nebula for its spidery appearance, the 30 Doradus complex is a monstrous stellar factory. It is the largest emission nebula in the sky, and can be seen far down in the southern sky at a distance of about 170,000 light-years, in the southern constellation Dorado (The Swordfish or the Goldfish). It is part of one of the Milky Way's neighbouring galaxies, the Large Magellanic Cloud. The Tarantula Nebula is thought to contain more than half a million times the mass of the Sun in gas and this vast, blazing labyrinth hosts some of the most massive stars known. The nebula owes its name to the arrangement of its brightest patches of nebulosity, that somewhat resemble the legs of a spider. They extend from a central 'body' where a cluster of hot stars (designated 'R136') illuminates and shapes the nebula. This name, of the biggest spiders on the Earth, is also very fitting in view of the gigantic proportions of the celestial nebula - it measures nearly 1,000 light-years across and extends over more than one third of a degree: almost, but not quite, the size of the full Moon. If it were in our own Galaxy, at the distance of another stellar nursery, the Orion Nebula (1,500 light-years away), it would cover one quarter of the sky and even be visible in daylight. Because astronomers believe that most of the stars in the Universe were formed in large and hectic nurseries such as the 30 Doradus region, its study is fundamental. Early this year, astronomers took a new, wide look at the spider and its web of filaments, using

Air Gaps, Size Effect, and Corner-Turning in Ambient LX-17

DOE Office of Scientific and Technical Information (OSTI.GOV)

Souers, P C; Hernandez, A; Cabacungen, C

2007-05-30

Various ambient measurements are presented for LX-17. The size (diameter) effect has been measured with copper and Lucite confinement, where the failure radii are 4.0 and 6.5 mm, respectively. The air well corner-turn has been measured with an LX-07 booster, and the dead-zone results are comparable to the previous TATB-boosted work. Four double cylinders have been fired, and dead zones appear in all cases. The steel-backed samples are faster than the Lucite-backed samples by 0.6 {micro}s. Bare LX-07 and LX-17 of 12.7 mm-radius were fired with air gaps. Long acceptor regions were used to truly determine if detonation occurred ormore » not. The LX-07 crossed at 10 mm with a slight time delay. Steady state LX-17 crossed at 3.5 mm gap but failed to cross at 4.0 mm. LX-17 with a 12.7 mm run after the booster crossed a 1.5 mm gap but failed to cross 2.5 mm. Timing delays were measured where the detonation crossed the gaps. The Tarantula model is introduced as embedded in the Linked Cheetah V4.0 reactive flow code at 4 zones/mm. Tarantula has four pressure regions: off, initiation, failure and detonation. A report card of 25 tests run with the same settings on LX-17 is shown, possibly the most extensive simultaneous calibration yet tried with an explosive. The physical basis of some of the input parameters is considered.« less

Less is More: Neural Activity During Very Brief and Clearly Visible Exposure to Phobic Stimuli

PubMed Central

Siegel, Paul; Warren, Richard; Wang, Zhishun; Yang, Jie; Cohen, Don; Anderson, Jason F.; Murray, Lilly; Peterson, Bradley S.

2017-01-01

Research on automatic processes in emotion has focused almost exclusively on the provocation of fear responses. We have shown, in contrast, that the repeated presentation of masked feared images reduces avoidance of a live tarantula by spider-phobic participants. We investigated the neural basis for these adaptive effects of masked exposure. We identified 21 spider-phobic and 21 control participants using a psychiatric interview, fear questionnaire, and approach behavior towards a live tarantula. They received each of three conditions: (1) very brief exposure (VBE) to masked images of spiders; (2) clearly visible exposure (CVE) to spiders; and (3) masked images of flowers (control). Only VBE to masked spiders generated neural activity more strongly in phobic than in control participants, within subcortical fear, attention, higher-order language, and vision systems. Counter-intuitively, CVE to the same spiders generated stronger neural activity in control rather than phobic participants within these and other systems. VBE activated regions that support fear processing in phobic participants without causing them to experience fear consciously. CVE, by contrast, deactivated regions supporting fear regulation and caused phobic participants to experience fear. Activations by CVE to spiders correlated with fear ratings - explicit fear. Activations by VBE to masked spiders correlated with color-naming interference of spider words – implicit fear. These multiple dissociations between the effects of VBE and CVE to spiders suggest that limiting awareness of exposure to phobic stimuli through visual masking paradoxically facilitates their processing, while simultaneously minimizing the experience of fear. PMID:28165171

A Massive Star Census of the Starburst Cluster R136

NASA Astrophysics Data System (ADS)

Crowther, Paul

2011-10-01

We propose to carry out a comprehensive census of the most massive stars in the central parsec {4"} of the starburst cluster, R136, which powers the Tarantula Nebula in the LMC. R136 is both sufficiently massive that the upper mass function is richly populated and young enough that its most massive stars have yet to explode as supernovae. The identification of very massive stars in R136, up to 300 solar masses, raises general questions of star formation, binarity and feedback in young massive clusters. The proposed STIS spectral survey of 36 stars more massive than 50 solar masses within R136 is ground-breaking, of legacy value, and is specifically tailored to a} yield physical properties; b} detect the majority of binaries by splitting observations between Cycles 19 and 20; c} measure rotational velocities, relevant for predictions of rotational mixing; d} quantify mass-loss properties for very massive stars; e} determine surface compositions; f} measure radial velocities, relevant for runaway stars and cluster dynamics; g} quantify radiative and mechanical feedback. This census will enable the mass function of very massive stars to be measured for the first time, as a result of incomplete and inadequate spectroscopy to date. It will also perfectly complement our Tarantula Survey, a ground-based VLT Large Programme, by including the most massive stars that are inaccessible to ground-based visual spectroscopy due to severe crowding. These surveys, together with existing integrated UV and optical studies will enable 30 Doradus to serve as a bona-fide template for unresolved extragalactic starburst regions.

A Massive Star Census of the Starburst Cluster R136

NASA Astrophysics Data System (ADS)

Crowther, Paul

2012-10-01

We propose to carry out a comprehensive census of the most massive stars in the central parsec {4"} of the starburst cluster, R136, which powers the Tarantula Nebula in the LMC. R136 is both sufficiently massive that the upper mass function is richly populated and young enough that its most massive stars have yet to explode as supernovae. The identification of very massive stars in R136, up to 300 solar masses, raises general questions of star formation, binarity and feedback in young massive clusters. The proposed STIS spectral survey of 36 stars more massive than 50 solar masses within R136 is ground-breaking, of legacy value, and is specifically tailored to a} yield physical properties; b} detect the majority of binaries by splitting observations between Cycles 19 and 20; c} measure rotational velocities, relevant for predictions of rotational mixing; d} quantify mass-loss properties for very massive stars; e} determine surface compositions; f} measure radial velocities, relevant for runaway stars and cluster dynamics; g} quantify radiative and mechanical feedback. This census will enable the mass function of very massive stars to be measured for the first time, as a result of incomplete and inadequate spectroscopy to date. It will also perfectly complement our Tarantula Survey, a ground-based VLT Large Programme, by including the most massive stars that are inaccessible to ground-based visual spectroscopy due to severe crowding. These surveys, together with existing integrated UV and optical studies will enable 30 Doradus to serve as a bona-fide template for unresolved extragalactic starburst regions.

Less is more: Neural activity during very brief and clearly visible exposure to phobic stimuli.

PubMed

Siegel, Paul; Warren, Richard; Wang, Zhishun; Yang, Jie; Cohen, Don; Anderson, Jason F; Murray, Lilly; Peterson, Bradley S

2017-05-01

Research on automatic processes in fear has emphasized the provocation of fear responses rather than their attenuation. We have previously shown that the repeated presentation of feared images without conscious awareness via backward masking reduces avoidance of a live tarantula in spider-phobic participants. Herein we investigated the neural basis for these adaptive effects of masked exposure. 21 spider-phobic and 21 control participants, identified by a psychiatric interview, fear questionnaire, and approaching a live tarantula, viewed stimuli in each of three conditions: (1) very brief exposure (VBE) to masked images of spiders, severely limited awareness; (2) clearly visible exposure (CVE) to spiders, full awareness; and (3) masked images of flowers (control), severely limited awareness. Only VBE to masked spiders generated neural activity more strongly in phobic than in control participants, within subcortical fear, attention, higher-order language, and vision systems. Moreover, VBE activated regions that support fear processing in phobic participants without causing them to experience fear consciously. Counter-intuitively, CVE to the same spiders generated stronger neural activity in control rather than phobic participants within these and other systems. CVE deactivated regions supporting fear regulation and caused phobic participants to experience fear. CVE-induced activations also correlated with measures of explicit fear ratings, whereas VBE-induced activations correlated with measures of implicit fear (color-naming interference of spider words). These multiple dissociations between the effects of VBE and CVE to spiders suggest that limiting awareness of exposure to phobic stimuli through visual masking paradoxically facilitates their processing, while simultaneously minimizing the experience of fear. Hum Brain Mapp 38:2466-2481, 2017. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

The dancing plague: a public health conundrum.

PubMed

Donaldson, L J; Cavanagh, J; Rankin, J

1997-07-01

The phenomenon of mass, frenzied dancing affected large populations in various parts of Europe from the thirteenth century and lasted, on and off, for three centuries. The exact aetiology of the Dancing Plague (or Dancing Mania) is still unclear. Retrospective historical review of this public health problem reveals claims for causative factors including demonic possession, epilepsy, the bite of a tarantula, ergot poisoning and social adversity. It seems unlikely that Dancing Mania resulted from a single cause but rather resulted from multiple factors combining with a predisposing cultural background and triggered by adverse social circumstances. Dancing Mania remains one of the unresolved mysteries of public health.

Properties of O dwarf stars in 30 Doradus

NASA Astrophysics Data System (ADS)

Sabín-Sanjulián, Carolina; VFTS Collaboration

2017-11-01

We perform a quantitative spectroscopic analysis of 105 presumably single O dwarf stars in 30 Doradus, located within the Large Magellanic Cloud. We use mid-to-high resolution multi-epoch optical spectroscopic data obtained within the VLT-FLAMES Tarantula Survey. Stellar and wind parameters are derived by means of the automatic tool iacob-gbat, which is based on a large grid of fastwind models. We also benefit from the Bayesian tool bonnsai to estimate evolutionary masses. We provide a spectral calibration for the effective temperature of O dwarf stars in the LMC, deal with the mass discrepancy problem and investigate the wind properties of the sample.

Massive binaries in R136 using Hubble

NASA Astrophysics Data System (ADS)

Caballero-Nieves, Saida; Crowther, Paul; Bostroem, K. Azalee; Maíz Apellániz, Jesus

2014-09-01

We have undertaken a complete HST/STIS spectroscopic survey of R136, the young, central dense starburst cluster of the LMC 30 Doradus nebula, which hosts the most massive stars currently known. Our CCD datasets, comprising 17 adjacent 0.2"×52" long slits, were split across Cycles 19 and 20 to allow us to search for spectroscopic binaries. We will present the results of our survey, including a comparison with the massive-star population in the wider 30 Doradus region from the VLT Flames Tarantula survey. We will also describe upcoming HST/FGS observations, which will probe intermediate-separation binaries in R136, and discuss this cluster in the context of unresolved young extragalactic star clusters.

Characterizing Detonating LX-17 Charges Crossing a Transverse Air Gap with Experiments and Modeling

NASA Astrophysics Data System (ADS)

Lauderbach, Lisa M.; Souers, P. Clark; Garcia, Frank; Vitello, Peter; Vandersall, Kevin S.

2009-12-01

Experiments were performed using detonating LX-17 (92.5% TATB, 7.5% Kel-F by weight) charges with various width transverse air gaps with manganin peizoresistive in-situ gauges present. The experiments, performed with 25 mm diameter by 25 mm long LX-17 pellets with the transverse air gap in between, showed that transverse gaps up to about 3 mm could be present without causing the detonation wave to fail to continue as a detonation. The Tarantula/JWL++ code was utilized to model the results and compare with the in-situ gauge records with some agreement to the experimental data with additional work needed for a better match to the data. This work will present the experimental details as well as comparison to the model results.

Controlling light with freeform optics: recent progress in computational methods for optical design of freeform lenses with prescribed irradiance properties

NASA Astrophysics Data System (ADS)

Oliker, Vladimir I.; Cherkasskiy, Boris

2014-09-01

Structural color is produced when nanostructures called schemochromes alter light reflected from a surface through different optic principles, in contrast with other types of colors that are produced when pigments selectively absorb certain wavelengths of light. Research on biogenic photonic nanostructures has focused primarily on bird feathers, butterfly wings and beetle elytra, ignoring other diverse groups such as spiders. We argue that spiders are a good model system to study the functions and evolution of colors in nature for the following reasons. First, these colors clearly function in spiders such as the tarantulas outside of sexual selection, which is likely the dominant driver of the evolution of structural colors in birds and butterflies. Second, within more than 44,000 currently known spider species, colors are used in every possible way based on the same sets of relatively simple materials. Using spiders, we can study how colors evolve to serve different functions under a variety of combinations of driving forces, and how those colors are produced within a relatively simple system. Here, we first review the different color-producing materials and mechanisms (i.e., light absorbing, reflecting and emitting) in birds, butterflies and beetles, the interactions between these different elements, and the functions of colors in different organisms. We then summarize the current state of knowledge of spider colors and compare it with that of birds and insects. We then raise questions including: 1. Could spiders use fluorescence as a mechanism to protect themselves from UV radiation, if they do not have the biosynthetic pathways to produce melanins? 2. What functions could color serve for nearly blind tarantulas? 3. Why are only multilayer nanostructures (thus far) found in spiders, while birds and butterflies use many diverse nanostructures? And, does this limit the diversity of structural colors found in spiders? Answering any of these questions in the future

Structural color and its interaction with other color-producing elements: perspectives from spiders

NASA Astrophysics Data System (ADS)

Hsiung, Bor-Kai; Blackledge, Todd A.; Shawkey, Matthew D.

2014-09-01

Structural color is produced when nanostructures called schemochromes alter light reflected from a surface through different optic principles, in contrast with other types of colors that are produced when pigments selectively absorb certain wavelengths of light. Research on biogenic photonic nanostructures has focused primarily on bird feathers, butterfly wings and beetle elytra, ignoring other diverse groups such as spiders. We argue that spiders are a good model system to study the functions and evolution of colors in nature for the following reasons. First, these colors clearly function in spiders such as the tarantulas outside of sexual selection, which is likely the dominant driver of the evolution of structural colors in birds and butterflies. Second, within more than 44,000 currently known spider species, colors are used in every possible way based on the same sets of relatively simple materials. Using spiders, we can study how colors evolve to serve different functions under a variety of combinations of driving forces, and how those colors are produced within a relatively simple system. Here, we first review the different color-producing materials and mechanisms (i.e., light absorbing, reflecting and emitting) in birds, butterflies and beetles, the interactions between these different elements, and the functions of colors in different organisms. We then summarize the current state of knowledge of spider colors and compare it with that of birds and insects. We then raise questions including: 1. Could spiders use fluorescence as a mechanism to protect themselves from UV radiation, if they do not have the biosynthetic pathways to produce melanins? 2. What functions could color serve for nearly blind tarantulas? 3. Why are only multilayer nanostructures (thus far) found in spiders, while birds and butterflies use many diverse nanostructures? And, does this limit the diversity of structural colors found in spiders? Answering any of these questions in the future

The effect of D-cycloserine on subliminal cue exposure in spider fearful individuals.

PubMed

Gutner, Cassidy A; Weinberger, Joel; Hofmann, Stefan G

2012-01-01

Research on D-cycloserine (DCS) has demonstrated a significant effect on symptom reduction in human studies that utilized conventional exposure-based approaches. Recent studies have offered promising results for targeting fears through subliminal paradigms. In this double-blind, randomized placebo-controlled study, 45 spider fearful individuals received DCS or placebo pills prior to completing a subliminal cue exposure task to images of spiders. Participants completed self-report questionnaires and a behavioral approach task to a live caged tarantula. After repeated exposure to subliminal spider cues, participants in the DCS group reported a greater reduction in disgust than individuals in the placebo group. No difference was observed in fear ratings. These findings suggest that DCS augments the reduction in disgust in spider fearful subjects after subliminal exposure to spider cues.

The Effect of D-cycloserine on Subliminal Cue Exposure in Spider Fearful Individuals

PubMed Central

Gutner, Cassidy A.; Weinberger, Joel; Hofmann, Stefan G.

2012-01-01

Research on d-cycloserine (DCS) has demonstrated a significant effect on symptom reduction in human studies that utilized conventional exposure-based approaches. Recent studies have offered promising results for targeting fears through subliminal paradigms. In this double-blind, randomized placebo controlled study, 45 spider fearful individuals received DCS or placebo pills prior to completing a subliminal cue exposure task to images of spiders. Participants completed self-report questionnaires and a behavioral approach task to a live caged tarantula. After repeated exposure to subliminal spider cues, participants in the DCS group reported a greater reduction in disgust than individuals in the placebo group. No difference was observed in fear ratings. These findings suggest that DCS augments the reduction in disgust in spider fearful subjects after subliminal exposure to spider cues. PMID:22992160

CHARACTERIZING DETONATING LX-17 CHARGES CROSSING A TRANSVERSE AIR GAP WITH EXPERIMENTS AND MODELING

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lauderbach, L M; Souers, P C; Garcia, F

2009-06-26

Experiments were performed using detonating LX-17 (92.5% TATB, 7.5% Kel-F by weight) charges with various width transverse air gaps with manganin peizoresistive in-situ gauges present. The experiments, performed with 25 mm diameter by 25 mm long LX-17 pellets with the transverse air gap in between, showed that transverse gaps up to about 3 mm could be present without causing the detonation wave to fail to continue as a detonation. The Tarantula/JWL{sup ++} code was utilized to model the results and compare with the in-situ gauge records with some agreement to the experimental data with additional work needed for a bettermore » match to the data. This work will present the experimental details as well as comparison to the model results.« less

The ultraviolet extinction properties of the 30 Dor Nebula

NASA Astrophysics Data System (ADS)

De Marchi, Guido; Panagia, Nino

2018-01-01

Recent investigation of the extinction law in 30 Dor and the Tarantula Nebula, at optical and near infrared wavelengths, has revealed a ratio of total to selective extinction RV=AV/E(B-V) of about 4.5. This indicates a larger proportion of large grains than in the Galactic diffuse interstellar medium. Possible origins include coalescence of small grains, grain growth, selective destruction of small grains, and fresh injection of large grains. From a study of the ultraviolet extinction properties of three Wolf-Rayet stars in 30 Dor (R 139, R 140, R 145), observed with the International Ultraviolet Explorer, we show that the excess of large grains does not come at the expense of small grains, which are still present. Fresh injection of large grains by supernova explosions appears to be the dominant mechanism.

The Aromatic Head Group of Spider Toxin Polyamines Influences Toxicity to Cancer Cells.

PubMed

Wilson, David; Boyle, Glen M; McIntyre, Lachlan; Nolan, Matthew J; Parsons, Peter G; Smith, Jennifer J; Tribolet, Leon; Loukas, Alex; Liddell, Michael J; Rash, Lachlan D; Daly, Norelle L

2017-10-27

Spider venoms constitute incredibly diverse libraries of compounds, many of which are involved in prey capture and defence. Polyamines are often prevalent in the venom and target ionotropic glutamate receptors. Here we show that a novel spider polyamine, PA 366 , containing a hydroxyphenyl-based structure is present in the venom of several species of tarantula, and has selective toxicity against MCF-7 breast cancer cells. By contrast, a polyamine from an Australian funnel-web spider venom, which contains an identical polyamine tail to PA 366 but an indole-based head-group, is only cytotoxic at high concentrations. Our results suggest that the ring structure plays a role in the cytotoxicity and that modification to the polyamine head group might lead to more potent and selective compounds with potential as novel cancer treatments.

The Aromatic Head Group of Spider Toxin Polyamines Influences Toxicity to Cancer Cells

PubMed Central

Wilson, David; McIntyre, Lachlan; Smith, Jennifer J.; Tribolet, Leon; Loukas, Alex; Liddell, Michael J.; Daly, Norelle L.

2017-01-01

Spider venoms constitute incredibly diverse libraries of compounds, many of which are involved in prey capture and defence. Polyamines are often prevalent in the venom and target ionotropic glutamate receptors. Here we show that a novel spider polyamine, PA366, containing a hydroxyphenyl-based structure is present in the venom of several species of tarantula, and has selective toxicity against MCF-7 breast cancer cells. By contrast, a polyamine from an Australian funnel-web spider venom, which contains an identical polyamine tail to PA366 but an indole-based head-group, is only cytotoxic at high concentrations. Our results suggest that the ring structure plays a role in the cytotoxicity and that modification to the polyamine head group might lead to more potent and selective compounds with potential as novel cancer treatments. PMID:29077051

Using humor in systematic desensitization to reduce fear.

PubMed

Ventis, W L; Higbee, G; Murdock, S A

2001-04-01

Effectiveness of systematic desensitization for fear reduction, using humorous hierarchy scenes without relaxation, was tested. Participants were 40 students highly fearful of spiders. Using a 24-item behavioral approach test with an American tarantula, participants were matched on fear level and randomly assigned to 1 of 3 treatment groups: (a) systematic desensitization, (b) humor desensitization, and (c) untreated controls. Each participant was seen for 6 sessions, including pretest and posttest. Analyses of covariance of posttest scores revealed that the 2 treatment groups showed greater reduction in fear than the controls on 3 measures but did not differ from each other. Therefore, humor in systematic desensitization reduced fear as effectively as more traditional desensitization. This finding may have therapeutic applications; however, it may also be applicable in advertising to desensitize fear of a dangerous product, such as cigarettes.

VizieR Online Data Catalog: Massive stars in 30 Dor (Schneider+, 2018)

NASA Astrophysics Data System (ADS)

Schneider, F. R. N.; Sana, H.; Evans, C. J.; Bestenlehner, J. M.; Castro, N.; Fossati, L.; Grafener, G.; Langer, N.; Ramirez-Agudelo, O. H.; Sabin-Sanjulian, C.; Simon-Diaz, S.; Tramper, F.; Crowther, P. A.; de Koter, A.; de Mink, S. E.; Dufton, P. L.; Garcia, M.; Gieles, M.; Henault-Brunet, V.; Herrero, A.; Izzard, R. G.; Kalari, V.; Lennon, D. J.; Apellaniz, J. M.; Markova, N.; Najarro, F.; Podsiadlowski, P.; Puls, J.; Taylor, W. D.; van Loon, J. T.; Vink, J. S.; Norman, C.

2018-02-01

Through the use of the Fibre Large Array Multi Element Spectrograph (FLAMES) on the Very Large Telescope (VLT), the VLT-FLAMES Tarantula Survey (VFTS) has obtained optical spectra of ~800 massive stars in 30 Dor, avoiding the core region of the dense star cluster R136 because of difficulties with crowding. Repeated observations at multiple epochs allow determination of the orbital motion of potentially binary objects. For a sample of 452 apparently single stars, robust stellar parameters-such as effective temperatures, luminosities, surface gravities, and projected rotational velocities-are determined by modeling the observed spectra. Composite spectra of visual multiple systems and spectroscopic binaries are not considered here because their parameters cannot be reliably inferred from the VFTS data. To match the derived atmospheric parameters of the apparently single VFTS stars to stellar evolutionary models, we use the Bayesian code Bonnsai. (2 data files).

Characterizing Detonating LX-17 Charges Crossing a Transverse Air Gap with Experiments and Modeling

NASA Astrophysics Data System (ADS)

Lauderbach, Lisa M.; Souers, P. Clark; Garcia, Frank; Vitello, Peter; Vandersall, Kevin S.

2009-06-01

Experiments were performed using detonating LX-17 (92.5% TATB, 7.5% Kel-f by weight) charges with various width transverse air gaps both with and without manganin peizoresistive in-situ gauges present. The experiments, performed with 25 mm diameter by 25 mm long LX-17 pellets with the transverse air gap in between, showed that transverse gaps up to about 3 mm could be present without causing the detonation wave to fail to continue as a detonation. A JWL++/Tarantula code was utilized to model the results and compare with the in-situ gauge records with reasonable agreement to the experimental data. This work will present the experimental details as well as comparison to the model results. This work performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344.

VizieR Online Data Catalog: VLT-FLAMES Tarantula Survey: B supergiants (McEvoy+, 2015)

NASA Astrophysics Data System (ADS)

McEvoy, C. M.; Dufton, P. L.; Evans, C. J.; Kalari, V. M.; Markova, N.; Simon-Diaz, S.; Vink, J. S.; Walborn, N. R.; Crowther, P. A.; de Koter, A.; de Mink, S. E.; Dunstall, P. R.; Henault-Brunet, V.; Herrero, A.; Langer, N.; Lennon, D. J.; Maiz Apellaniz, J.; Najarro, F.; Puls, J.; Sana, H.; Schneider, F. R. N.; Taylor, W. D.

2015-06-01

The majority of the VFTS data were obtained using the Medusa mode of FLAMES, which uses fibres to feed the light from up to 130 targets simultaneously to the Giraffe spectrograph. Nine Medusa configurations (Fields A to I) were observed in the 30 Dor region, with the targets sampling its different clusters and the local field population. The analysis presented here employs the FLAMES-Medusa observations obtained with two of the standard Giraffe settings (LR02 and LR03), giving coverage of the λλ3960-5050Å region at a resolving power of ~7000. (5 data files).

The 155-day X-ray cycle of the very massive Wolf-Rayet star Melnick 34 in the Large Magellanic Cloud

NASA Astrophysics Data System (ADS)

Pollock, A. M. T.; Crowther, P. A.; Tehrani, K.; Broos, Patrick S.; Townsley, Leisa K.

2018-03-01

The Wolf-Rayet star Mk 34 was observed more than 50 times as part of the deep T-ReX Chandra ACIS-I X-ray imaging survey of the Tarantula Nebula in the Large Magellanic Cloud conducted between 2014 May and 2016 January. Its brightness showed one bright maximum and repeated faint minima which help define an X-ray recurrence time of 155.1 ± 0.1 d that is probably the orbital period of an eccentric binary system. The maximum immediately precedes the minimum in the folded X-ray light curve as confirmed by new Swift XRT observations. Notwithstanding its extreme median luminosity of 1.2 × 1035 erg s-1, which makes it over an order of magnitude brighter than comparable stars in the Milky Way, Mk 34 is almost certainly a colliding-wind binary system. Its spectrum shows phase-related changes of luminosity and absorption that are probably related to the orbital dynamics of two of the most massive stars known.

Spider genomes provide insight into composition and evolution of venom and silk

PubMed Central

Sanggaard, Kristian W.; Bechsgaard, Jesper S.; Fang, Xiaodong; Duan, Jinjie; Dyrlund, Thomas F.; Gupta, Vikas; Jiang, Xuanting; Cheng, Ling; Fan, Dingding; Feng, Yue; Han, Lijuan; Huang, Zhiyong; Wu, Zongze; Liao, Li; Settepani, Virginia; Thøgersen, Ida B.; Vanthournout, Bram; Wang, Tobias; Zhu, Yabing; Funch, Peter; Enghild, Jan J.; Schauser, Leif; Andersen, Stig U.; Villesen, Palle; Schierup, Mikkel H; Bilde, Trine; Wang, Jun

2014-01-01

Spiders are ecologically important predators with complex venom and extraordinarily tough silk that enables capture of large prey. Here we present the assembled genome of the social velvet spider and a draft assembly of the tarantula genome that represent two major taxonomic groups of spiders. The spider genomes are large with short exons and long introns, reminiscent of mammalian genomes. Phylogenetic analyses place spiders and ticks as sister groups supporting polyphyly of the Acari. Complex sets of venom and silk genes/proteins are identified. We find that venom genes evolved by sequential duplication, and that the toxic effect of venom is most likely activated by proteases present in the venom. The set of silk genes reveals a highly dynamic gene evolution, new types of silk genes and proteins, and a novel use of aciniform silk. These insights create new opportunities for pharmacological applications of venom and biomaterial applications of silk. PMID:24801114

A verified spider bite and a review of the literature confirm Indian ornamental tree spiders (Poecilotheria species) as underestimated theraphosids of medical importance.

PubMed

Fuchs, Joan; von Dechend, Margot; Mordasini, Raffaella; Ceschi, Alessandro; Nentwig, Wolfgang

2014-01-01

Literature on bird spider or tarantula bites (Theraphosidae) is rare. This is astonishing as they are coveted pets and interaction with their keepers (feeding, cleaning the terrarium or taking them out to hold) might increase the possibility for bites. Yet, this seems to be a rare event and might be why most theraphosids are considered to be harmless, even though the urticating hairs of many American species can cause disagreeable allergic reactions. We are describing a case of a verified bite by an Indian ornamental tree spider (Poecilotheria regalis), where the patient developed severe, long lasting muscle cramps several hours after the bite. We present a comprehensive review of the literature on bites of these beautiful spiders and conclude that a delayed onset of severe muscle cramps, lasting for days, is characteristic for Poecilotheria bites. We discuss Poecilotheria species as an exception from the general assumption that theraphosid bites are harmless to humans. Copyright © 2013 Elsevier Ltd. All rights reserved.

Engineering vanilloid-sensitivity into the rat TRPV2 channel.

PubMed

Zhang, Feng; Hanson, Sonya M; Jara-Oseguera, Andres; Krepkiy, Dmitriy; Bae, Chanhyung; Pearce, Larry V; Blumberg, Peter M; Newstead, Simon; Swartz, Kenton J

2016-05-13

The TRPV1 channel is a detector of noxious stimuli, including heat, acidosis, vanilloid compounds and lipids. The gating mechanisms of the related TRPV2 channel are poorly understood because selective high affinity ligands are not available, and the threshold for heat activation is extremely high (>50°C). Cryo-EM structures of TRPV1 and TRPV2 reveal that they adopt similar structures, and identify a putative vanilloid binding pocket near the internal side of TRPV1. Here we use biochemical and electrophysiological approaches to investigate the resiniferatoxin(RTx) binding site in TRPV1 and to explore the functional relationships between TRPV1 and TRPV2. Collectively, our results support the interaction of vanilloids with the proposed RTx binding pocket, and demonstrate an allosteric influence of a tarantula toxin on vanilloid binding. Moreover, we show that sensitivity to RTx can be engineered into TRPV2, demonstrating that the gating and permeation properties of this channel are similar to TRPV1.

Mites Parasitic on Australasian and African Spiders Found in the Pet Trade; a Redescription of Ljunghia pulleinei Womersley

PubMed Central

Masan, Peter; Simpson, Christopher; Perotti, M. Alejandra; Braig, Henk R.

2012-01-01

Parasitic mites associated with spiders are spreading world-wide through the trade in tarantulas and other pet species. Ljunghia pulleinei Womersley, a mesostigmatic laelapid mite originally found in association with the mygalomorph spider Selenocosmia stirlingi Hogg (Theraphosidae) in Australia, is redescribed and illustrated on the basis of specimens from the African theraphosid spider Pterinochilus chordatus (Gerstäcker) kept in captivity in the British Isles (Wales). The mite is known from older original descriptions of Womersley in 1956; the subsequent redescription of Domrow in 1975 seems to be questionable in conspecificity of treated specimens with the type material. Some inconsistencies in both descriptions are recognised here as intraspecific variability of the studied specimens. The genus Arachnyssus Ma, with species A. guangxiensis (type) and A. huwenae, is not considered to be a valid genus, and is included in synonymy with Ljunghia Oudemans. A new key to world species of the genus Ljunghia is provided. PMID:22720019

Ambitious Survey Spots Stellar Nurseries

NASA Astrophysics Data System (ADS)

2010-08-01

Astronomers scanning the skies as part of ESO's VISTA Magellanic Cloud survey have now obtained a spectacular picture of the Tarantula Nebula in our neighbouring galaxy, the Large Magellanic Cloud. This panoramic near-infrared view captures the nebula itself in great detail as well as the rich surrounding area of sky. The image was obtained at the start of a very ambitious survey of our neighbouring galaxies, the Magellanic Clouds, and their environment. The leader of the survey team, Maria-Rosa Cioni (University of Hertfordshire, UK) explains: "This view is of one of the most important regions of star formation in the local Universe - the spectacular 30 Doradus star-forming region, also called the Tarantula Nebula. At its core is a large cluster of stars called RMC 136, in which some of the most massive stars known are located." ESO's VISTA telescope [1] is a new survey telescope at the Paranal Observatory in Chile (eso0949). VISTA is equipped with a huge camera that detects light in the near-infrared part of the spectrum, revealing a wealth of detail about astronomical objects that gives us insight into the inner workings of astronomical phenomena. Near-infrared light has a longer wavelength than visible light and so we cannot see it directly for ourselves, but it can pass through much of the dust that would normally obscure our view. This makes it particularly useful for studying objects such as young stars that are still enshrouded in the gas and dust clouds from which they formed. Another powerful aspect of VISTA is the large area of the sky that its camera can capture in each shot. This image is the latest view from the VISTA Magellanic Cloud Survey (VMC). The project will scan a vast area - 184 square degrees of the sky (corresponding to almost one thousand times the apparent area of the full Moon) including our neighbouring galaxies the Large and Small Magellanic Clouds. The end result will be a detailed study of the star formation history and three

VizieR Online Data Catalog: VLT-FLAMES Tarantula Survey. 30 Dor luminous stars (Doran+, 2013)

NASA Astrophysics Data System (ADS)

Doran, E. I.; Crowther, P. A.; de Koter, A.; Evans, C. J.; McEvoy, C.; Walborn, N. R.; Bastian, N.; Bestenlehner, J. M.; Grafener, G.; Herrero, A.; Kohler, K.; Maiz Apellaniz, J.; Najarro, F.; Puls, J.; Sana, H.; Schneider, F. R. N.; Taylor, W. D.; van Loon, J. T.; Vink, J. S.

2013-08-01

A census was compiled of all the hot luminous stars within the central 10 arcminutes of 30 Doradus. Candidate hot luminous stars were selected from a series of photometric catalogues, using a set of criteria explained in the paper. All stars meeting this photometric criteria are listed in Tabled1.dat. In addition, Table D1 includes all known Wolf-Rayet and Of/WN stars in the region, which may not have been selected due to photometric effects. Spectral Types were then matched to as many of the candidate stars in Tabled1.dat as possible. Stellar parameters were determined for all stars with the following spectral types: W-R, Of/WN, O-type, B-supergiant, B-giant B1I or earlier, B-dwarf, B0.5V or earlier. These parameters are listed in Tabled2.dat. Parameters of all O-type and B-type stars were derived through various calibrations. Parameters of W-R and Of/WN stars were based on previous work or various template models explained in the paper. (2 data files).

Porting Inition and Failure to Linked Cheetah

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vitello, P; Souers, P C

2007-07-18

Linked CHEETAH is a thermo-chemical code coupled to a 2-D hydrocode. Initially, a quadratic-pressure dependent kinetic rate was used, which worked well in modeling prompt detonation of explosives of large size, but does not work on other aspects of explosive behavior. The variable-pressure Tarantula reactive flow rate model was developed with JWL++ in order to also describe failure and initiation, and we have moved this model into Linked CHEETAH. The model works by turning on only above a pressure threshold, where a slow turn-on creates initiation. At a higher pressure, the rate suddenly leaps to a large value over amore » small pressure range. A slowly failing cylinder will see a rapidly declining rate, which pushes it quickly into failure. At a high pressure, the detonation rate is constant. A sequential validation procedure is used, which includes metal-confined cylinders, rate-sticks, corner-turning, initiation and threshold, gap tests and air gaps. The size (diameter) effect is central to the calibration.« less

Revised generic placement of Brachypelma embrithes (Chamberlin & Ivie, 1936) and Brachypelma angustum Valerio, 1980, with definition of the taxonomic features for identification of female Sericopelma Ausserer, 1875 (Araneae, Theraphosidae)

PubMed Central

Gabriel, Ray; Longhorn, Stuart J.

2015-01-01

Abstract The tarantula genus Sericopelma was originally defined based on male specimens, most notably lacking tibial spurs on leg I. Early female specimens were unrecognised as Sericopelma, and typically placed in Eurypelma – a dumping ground for problem specimens. The first females were only later recognised, but authors failed to adequately define female Sericopelma. Here, the holotypes of the southern-most alleged Brachypelma species, Brachypelma embrithes (Chamberlin & Ivie, 1936) and Brachypelma angustum Valerio, 1980 were examined, and finding both to possess defining characteristics of Sericopelma were transferred. The taxonomic attributes to define Sericopelma relative to Brachypelma and select other Neotropical genera are discussed, especially for females. As important diagnostic characters for Sericopelma, the single (unilobar) spermathecae swollen at the apex forming a P-shaped cross-section, metatarsus IV with trace scopula, femur IV with a dense retrolateral pad of plumose hair, plus other attributes. Some past confusion in these characters are clarified and Sericopelma relative to Brachypelma and Megaphobema mesomelas are discussed. Finally recommendations are given about these taxonomic changes for CITES regulations. PMID:26487826

An external sodium ion binding site controls allosteric gating in TRPV1 channels

PubMed Central

Jara-Oseguera, Andres; Bae, Chanhyung; Swartz, Kenton J

2016-01-01

TRPV1 channels in sensory neurons are integrators of painful stimuli and heat, yet how they integrate diverse stimuli and sense temperature remains elusive. Here, we show that external sodium ions stabilize the TRPV1 channel in a closed state, such that removing the external ion leads to channel activation. In studying the underlying mechanism, we find that the temperature sensors in TRPV1 activate in two steps to favor opening, and that the binding of sodium to an extracellular site exerts allosteric control over temperature-sensor activation and opening of the pore. The binding of a tarantula toxin to the external pore also exerts control over temperature-sensor activation, whereas binding of vanilloids influences temperature-sensitivity by largely affecting the open/closed equilibrium. Our results reveal a fundamental role of the external pore in the allosteric control of TRPV1 channel gating and provide essential constraints for understanding how these channels can be tuned by diverse stimuli. DOI: http://dx.doi.org/10.7554/eLife.13356.001 PMID:26882503

Engineering vanilloid-sensitivity into the rat TRPV2 channel

PubMed Central

Zhang, Feng; Hanson, Sonya M; Jara-Oseguera, Andres; Krepkiy, Dmitriy; Bae, Chanhyung; Pearce, Larry V; Blumberg, Peter M; Newstead, Simon; Swartz, Kenton J

2016-01-01

The TRPV1 channel is a detector of noxious stimuli, including heat, acidosis, vanilloid compounds and lipids. The gating mechanisms of the related TRPV2 channel are poorly understood because selective high affinity ligands are not available, and the threshold for heat activation is extremely high (>50°C). Cryo-EM structures of TRPV1 and TRPV2 reveal that they adopt similar structures, and identify a putative vanilloid binding pocket near the internal side of TRPV1. Here we use biochemical and electrophysiological approaches to investigate the resiniferatoxin(RTx) binding site in TRPV1 and to explore the functional relationships between TRPV1 and TRPV2. Collectively, our results support the interaction of vanilloids with the proposed RTx binding pocket, and demonstrate an allosteric influence of a tarantula toxin on vanilloid binding. Moreover, we show that sensitivity to RTx can be engineered into TRPV2, demonstrating that the gating and permeation properties of this channel are similar to TRPV1. DOI: http://dx.doi.org/10.7554/eLife.16409.001 PMID:27177419

Relating Line Width and Optical Depth for CO Emission in the Large Mgellanic Cloud

NASA Astrophysics Data System (ADS)

Wojciechowski, Evan; Wong, Tony; Bandurski, Jeffrey; MC3 (Mapping CO in Molecular Clouds in the Magellanic Clouds) Team

2018-01-01

We investigate data produced from ALMA observations of giant molecular clouds (GMCs) located in the Large Magellanic Cloud (LMC), using 12CO(2–1) and 13CO(2–1) emission. The spectral line width is generally interpreted as tracing turbulent rather than thermal motions in the cloud, but could also be affected by optical depth, especially for the 12CO line (Hacar et al. 2016). We compare the spectral line widths of both lines with their optical depths, estimated from an LTE analysis, to evaluate the importance of optical depth effects. Our cloud sample includes two regions recently published by Wong et al. (2017, submitted): the Tarantula Nebula or 30 Dor, an HII region rife with turbulence, and the Planck cold cloud (PCC), located in a much calmer environment near the fringes of the LMC. We also include four additional LMC clouds, which span intermediate levels of star formation relative to these two clouds, and for which we have recently obtained ALMA data in Cycle 4.

Antihyperalgesic effects of ProTx-II, a Nav1.7 antagonist, and A803467, a Nav1.8 antagonist, in diabetic mice.

PubMed

Tanaka, Ken-Ichiro; Sekino, Shota; Ikegami, Megumi; Ikeda, Hiroko; Kamei, Junzo

2015-01-01

The present study investigated the effects of intrathecal administration of ProTx-II (tarantula venom peptide) and A803467 (5-[4-chloro-phenyl]-furan-2-carboxylic acid [3,5-dimethoxy-phenyl]-amide), selective Nav1.7 and Nav1.8 antagonists, respectively, on thermal hyperalgesia in a painful diabetic neuropathy model of mice. Intrathecal administration of ProTx-II at doses from 0.04 to 4 ng to diabetic mice dose-dependently and significantly increased the tail-flick latency. Intrathecal administration of A803467 at doses from 10 to 100 ng to diabetic mice also dose-dependently and significantly increased the tail-flick latency. However, intrathecal administration of either ProTx-II (4 ng) or A803467 (100 ng) had no effect on the tail-flick latency in nondiabetic mice. The expression of either the Nav1.7 or Nav1.8 sodium channel protein in the dorsal root ganglion in diabetic mice was not different from that in nondiabetic mice. The present results suggest that ProTx-II and A803467, highly selective blockers of Nav1.7 and Nav1.8 sodium channels, respectively, in the spinal cord, can have antihyperalgesic effects in diabetic mice.

Porting Initiation and Failure into Linked CHEETAH

NASA Astrophysics Data System (ADS)

Souers, Clark; Vitello, Peter

2007-06-01

Linked CHEETAH is a thermo-chemical code coupled to a 2-D hydrocode. Initially, a quadratic-pressure dependent kinetic rate was used, which worked well in modeling prompt detonation of explosives of large size, but does not work on other aspects of explosive behavior. The variable-pressure Tarantula reactive flow rate model was developed with JWL++ in order to also describe failure and initiation, and we have moved this model into Linked CHEETAH. The model works by turning on only above a pressure threshold, where a slow turn-on creates initiation. At a higher pressure, the rate suddenly leaps to a large value over a small pressure range. A slowly failing cylinder will see a rapidly declining rate, which pushes it quickly into failure. At a high pressure, the detonation rate is constant. A sequential validation procedure is used, which includes metal-confined cylinders, rate-sticks, corner-turning, initiation and threshold, gap tests and air gaps. The size (diameter) effect is central to the calibration. This work was performed under the auspices of the U.S. Department of Energy by the University of California Lawrence Livermore National Laboratory under contract No. W-7405-Eng-48.

Specificity of disgust sensitivity in the prediction of fear and disgust responding to a brief spider exposure.

PubMed

Olatunji, Bunmi O; Deacon, Brett

2008-01-01

This study examined the specificity of disgust sensitivity in predicting fear and disgust responses to exposure to a spider. Participants high (n=22) and low (n=28) in spider fear completed self-report measures of disgust sensitivity, contamination fear, anxiety, and negative affect. They then participated in a behavioral avoidance task (BAT) in which they were briefly exposed to a realistic-looking, but fake, tarantula. Results revealed that disgust sensitivity was associated with fear and disgust responding to the BAT. The association between disgust sensitivity and disgust responding to the BAT remained significant after controlling for gender, spider fear group membership, contamination fear, anxiety, and negative affect. However, the association between disgust sensitivity and fear responding to the BAT was only marginally significant after controlling for the same variables. Contamination fear was also strongly related to fear and disgust responding to the BAT. However, this relationship was fully mediated by disgust sensitivity. These findings indicate that disgust sensitivity has a unique association with aversive responding to spiders. The implications of these findings for better understanding the complex role of fear and disgust as they related to disgust sensitivity in spider phobia are discussed.

Experimental flights using a small unmanned aircraft system for mapping emergent sandbars

USGS Publications Warehouse

Kinzel, Paul J.; Bauer, Mark A.; Feller, Mark R.; Holmquist-Johnson, Christopher; Preston, Todd

2015-01-01

The US Geological Survey and Parallel Inc. conducted experimental flights with the Tarantula Hawk (T-Hawk) unmanned aircraft system (UAS ) at the Dyer and Cottonwood Ranch properties located along reaches of the Platte River near Overton, Nebraska, in July 2013. We equipped the T-Hawk UAS platform with a consumer-grade digital camera to collect imagery of emergent sandbars in the reaches and used photogrammetric software and surveyed control points to generate orthophotographs and digital elevation models (DEMS ) of the reaches. To optimize the image alignment process, we retained and/or eliminated tie points based on their relative errors and spatial resolution, whereby minimizing the total error in the project. Additionally, we collected seven transects that traversed emergent sandbars concurrently with global positioning system location data to evaluate the accuracy of the UAS survey methodology. The root mean square errors for the elevation of emergent points along each transect across the DEMS ranged from 0.04 to 0.12 m. If adequate survey control is established, a UAS combined with photogrammetry software shows promise for accurate monitoring of emergent sandbar morphology and river management activities in short (1–2 km) river reaches.

Snake oil and venoms for medical research

NASA Astrophysics Data System (ADS)

Wolpert, H. D.

2011-04-01

Some think that using derivatives of snake venom for medical purposes is the modern version of snake oil but they are seriously misjudging the research potentials of some of these toxins in medicines of the 2000's. Medical trials, using some of the compounds has proven their usefulness. Several venoms have shown the possibilities that could lead to anticoagulants, helpful in heart disease. The blood clotting protein from the taipan snake has been shown to rapidly stop excessive bleeding. The venom from the copperhead may hold an answer to breast cancer. The Malaysian pit viper shows promise in breaking blood clots. Cobra venom may hold keys to finding cures for Parkinson's disease and Alzheimer's. Rattlesnake proteins from certain species have produced blood pressure medicines. Besides snake venoms, venom from the South American dart frog, mollusks (i.e. Cone Shell Snail), lizards (i.e. Gila Monster & Komodo Dragon), some species of spiders and tarantulas, Cephalopods, mammals (i.e. Platypus & Shrews), fish (i.e. sting rays, stone fish, puffer fish, blue bottle fish & box jelly fish), intertidal marine animals (echinoderms)(i.e. Crown of Thorn Star Fish & Flower Urchin) and the Honeybee are being investigated for potential medical benefits.

Hubble Peers into the Storm

NASA Image and Video Library

2017-12-08

This shot from the NASA/ESA Hubble Space Telescope shows a maelstrom of glowing gas and dark dust within one of the Milky Way’s satellite galaxies, the Large Magellanic Cloud (LMC). This stormy scene shows a stellar nursery known as N159, an HII region over 150 light-years across. N159 contains many hot young stars. These stars are emitting intense ultraviolet light, which causes nearby hydrogen gas to glow, and torrential stellar winds, which are carving out ridges, arcs, and filaments from the surrounding material. At the heart of this cosmic cloud lies the Papillon Nebula, a butterfly-shaped region of nebulosity. This small, dense object is classified as a High-Excitation Blob, and is thought to be tightly linked to the early stages of massive star formation. N159 is located over 160,000 light-years away. It resides just south of the Tarantula Nebula (heic1402), another massive star-forming complex within the LMC. This image comes from Hubble’s Advanced Camera for Surveys. The region was previously imaged by Hubble’s Wide Field Planetary Camera 2, which also resolved the Papillon Nebula for the first time. Credit: ESA/Hubble & NASA

Pharmacological characterisation of the highly NaV1.7 selective spider venom peptide Pn3a.

PubMed

Deuis, Jennifer R; Dekan, Zoltan; Wingerd, Joshua S; Smith, Jennifer J; Munasinghe, Nehan R; Bhola, Rebecca F; Imlach, Wendy L; Herzig, Volker; Armstrong, David A; Rosengren, K Johan; Bosmans, Frank; Waxman, Stephen G; Dib-Hajj, Sulayman D; Escoubas, Pierre; Minett, Michael S; Christie, Macdonald J; King, Glenn F; Alewood, Paul F; Lewis, Richard J; Wood, John N; Vetter, Irina

2017-01-20

Human genetic studies have implicated the voltage-gated sodium channel Na V 1.7 as a therapeutic target for the treatment of pain. A novel peptide, μ-theraphotoxin-Pn3a, isolated from venom of the tarantula Pamphobeteus nigricolor, potently inhibits Na V 1.7 (IC 50 0.9 nM) with at least 40-1000-fold selectivity over all other Na V subtypes. Despite on-target activity in small-diameter dorsal root ganglia, spinal slices, and in a mouse model of pain induced by Na V 1.7 activation, Pn3a alone displayed no analgesic activity in formalin-, carrageenan- or FCA-induced pain in rodents when administered systemically. A broad lack of analgesic activity was also found for the selective Na V 1.7 inhibitors PF-04856264 and phlotoxin 1. However, when administered with subtherapeutic doses of opioids or the enkephalinase inhibitor thiorphan, these subtype-selective Na V 1.7 inhibitors produced profound analgesia. Our results suggest that in these inflammatory models, acute administration of peripherally restricted Na V 1.7 inhibitors can only produce analgesia when administered in combination with an opioid.

Pharmacological characterisation of the highly NaV1.7 selective spider venom peptide Pn3a

PubMed Central

Deuis, Jennifer R.; Dekan, Zoltan; Wingerd, Joshua S.; Smith, Jennifer J.; Munasinghe, Nehan R.; Bhola, Rebecca F.; Imlach, Wendy L.; Herzig, Volker; Armstrong, David A.; Rosengren, K. Johan; Bosmans, Frank; Waxman, Stephen G.; Dib-Hajj, Sulayman D.; Escoubas, Pierre; Minett, Michael S.; Christie, Macdonald J.; King, Glenn F.; Alewood, Paul F.; Lewis, Richard J.; Wood, John N.; Vetter, Irina

2017-01-01

Human genetic studies have implicated the voltage-gated sodium channel NaV1.7 as a therapeutic target for the treatment of pain. A novel peptide, μ-theraphotoxin-Pn3a, isolated from venom of the tarantula Pamphobeteus nigricolor, potently inhibits NaV1.7 (IC50 0.9 nM) with at least 40–1000-fold selectivity over all other NaV subtypes. Despite on-target activity in small-diameter dorsal root ganglia, spinal slices, and in a mouse model of pain induced by NaV1.7 activation, Pn3a alone displayed no analgesic activity in formalin-, carrageenan- or FCA-induced pain in rodents when administered systemically. A broad lack of analgesic activity was also found for the selective NaV1.7 inhibitors PF-04856264 and phlotoxin 1. However, when administered with subtherapeutic doses of opioids or the enkephalinase inhibitor thiorphan, these subtype-selective NaV1.7 inhibitors produced profound analgesia. Our results suggest that in these inflammatory models, acute administration of peripherally restricted NaV1.7 inhibitors can only produce analgesia when administered in combination with an opioid. PMID:28106092

New HST/STIS Spectroscopy of Massive Members of R136 in 30 Doradus

NASA Astrophysics Data System (ADS)

Bostroem, Kyra; Walborn, Nolan; Crowther, Paul; Caballero-Nieves, Saida; Lennon, Daniel; Maíz Apellániz, Jesús

2013-06-01

We display new (in some cases, the first ever) spatially resolved optical and UV spectroscopy of a number of early O-type stars in R136, the massive core cluster of 30 Doradus in the LMC. Some of them are of the earliest spectral types, O2-O3, which accompany the more luminous WN members that are the most massive stars known, near or exceeding 300~M_⊙ initially. These results are relevant to the very top of the IMF and to the structure and formation of starburst clusters. The data are from HST/STIS programs GO 12465/13052 (PI Crowther), in which the long slit was stepped across the inner 4 arcsec (1 parsec) of R136, yielding both optical photospheric and FUV stellar-wind spectra of at least 100 resolved members, many of them for the first time. The optical data were obtained at 4 epochs to support eventual radial-velocity detection of spectroscopic binaries. This program vitally complements the VLT-FLAMES Tarantula Survey of the wider stellar content of 30 Doradus, by adding that of the massive core cluster, which is inaccessible to such observations from the ground. These combined datasets will provide unprecedented information about massive stellar evolution and starbursts.

Superluminous object in the Large Cloud of Magellan

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mathis, J.S.; Savage, B.D.; Cassinelli, J.P.

1984-08-01

A superluminous and possibly supermassive object has been observed in the Large Cloud of Magellan. The object is designated R136 and is in the Tarantula Nebula. In 1980, it was discovered that R136 actually has 3 distinct components. The brightest was designated R136a. The ultraviolet spectra of R136a implies that it is a very hot star, similar to 03 stars, and that it has a steller wind of speeds up 3500 km/sec., also similar to 03 stars. The broad emission lines of the II are similar to those found in the spectrum of Wolf-Rayet stars. In 1983, Y.H. Chu ofmore » the University of Wisconsin after analyzing many images of R136 concluded that within the R136a component one can identify four steller objects. The dominate object was labeled R136a1 and it is this object that is now the candidate for a superluminous star. If R136a1 is a single star, it must have a mass of between 400 and 1000 solar masses. The ultraviolet spectroscopic data are consistent with a single-star hypothesis. However, the data do not rule out other possibilities.« less

Discovering the silk road: Nuclear and mitochondrial sequence data resolve the phylogenetic relationships among theraphosid spider subfamilies.

PubMed

Lüddecke, Tim; Krehenwinkel, Henrik; Canning, Gregory; Glaw, Frank; Longhorn, Stuart J; Tänzler, René; Wendt, Ingo; Vences, Miguel

2018-02-01

The mygalomorph spiders in the family Theraphosidae, also known as "tarantulas", are one of the most popular and diverse groups of arachnids, but their evolutionary history remains poorly understood because morphological analyses have only provided mostly controversial results, and a broad molecular perspective has been lacking until now. In this study we provide a preliminary molecular phylogenetic hypothesis of relationships among theraphosid subfamilies, based on 3.5 kbp of three nuclear and three mitochondrial markers, for 52 taxa representing 10 of the 11 commonly accepted subfamilies. Our analysis confirms the monophyly of the Theraphosidae and of most recognized theraphosid subfamilies, supports the validity of the Stromatopelminae and Poecilotheriinae, and indicates paraphyly of the Schismatothelinae. The placement of representatives of Schismatothelinae also indicates possible non-monophyly of Aviculariinae and supports the distinction of the previously contentious subfamily Psalmopoeinae. Major clades typically corresponded to taxa occurring in the same biogeographic region, with two of them each occurring in Africa, South America and Asia. Because relationships among these major clades were poorly supported, more extensive molecular data sets are required to test the hypothesis of independent colonization and multiple dispersal events among these continents. Copyright © 2017 Elsevier Inc. All rights reserved.

Effects of the antimicrobial peptide gomesin on the global gene expression profile, virulence and biofilm formation of Xylella fastidiosa.

PubMed

Fogaça, Andréa C; Zaini, Paulo A; Wulff, Nelson A; da Silva, Patrícia I P; Fázio, Marcos A; Miranda, Antônio; Daffre, Sirlei; da Silva, Aline M

2010-05-01

In the xylem vessels of susceptible hosts, such as citrus trees, Xylella fastidiosa forms biofilm-like colonies that can block water transport, which appears to correlate to disease symptoms. Besides aiding host colonization, bacterial biofilms play an important role in resistance against antimicrobial agents, for instance antimicrobial peptides (AMPs). Here, we show that gomesin, a potent AMP from a tarantula spider, modulates X. fastidiosa gene expression profile upon 60 min of treatment with a sublethal concentration. DNA microarray hybridizations revealed that among the upregulated coding sequences, some are related to biofilm production. In addition, we show that the biofilm formed by gomesin-treated bacteria is thicker than that formed by nontreated cells or cells exposed to streptomycin. We have also observed that the treatment of X. fastidiosa with a sublethal concentration of gomesin before inoculation in tobacco plants correlates with a reduction in foliar symptoms, an effect possibly due to the trapping of bacterial cells to fewer xylem vessels, given the enhancement in biofilm production. These results warrant further investigation of how X. fastidiosa would respond to the AMPs produced by citrus endophytes and by the insect vector, leading to a better understanding of the mechanism of action of these molecules on bacterial virulence.

An Unsung Fashion Designer, Congo Square, Pinktoe Tarantulas, and Dead People: Expecting the Unexpected in Children's Literature

ERIC Educational Resources Information Center

McNair, Jonda C.

2017-01-01

Children's literature plays an essential role in the literacy development of children. This department column focuses on the teaching and use of children's literature and provides educators with information about a wide range of books across multiple genres that are representative of the diverse world in which we live. A strong emphasis is placed…

78 FR 72622 - Endangered and Threatened Wildlife and Plants; 90-Day Finding on a Petition To List 11 Tarantula...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-03

... attach multiple pieces of information, our preferred format is a spreadsheet in Microsoft Excel. By hard... addresses the petition. Evaluation of Petition The 11 species named in the petition include six species... species is endemic to the southern Western Ghats, in southern India. The species is reported from six...

The Tarantula Massive Binary Monitoring. II. First SB2 orbital and spectroscopic analysis for the Wolf-Rayet binary R145

NASA Astrophysics Data System (ADS)

Shenar, T.; Richardson, N. D.; Sablowski, D. P.; Hainich, R.; Sana, H.; Moffat, A. F. J.; Todt, H.; Hamann, W.-R.; Oskinova, L. M.; Sander, A.; Tramper, F.; Langer, N.; Bonanos, A. Z.; de Mink, S. E.; Gräfener, G.; Crowther, P. A.; Vink, J. S.; Almeida, L. A.; de Koter, A.; Barbá, R.; Herrero, A.; Ulaczyk, K.

2017-02-01

We present the first SB2 orbital solution and disentanglement of the massive Wolf-Rayet binary R145 (P = 159 d) located in the Large Magellanic Cloud. The primary was claimed to have a stellar mass greater than 300 M⊙, making it a candidate for being the most massive star known to date. While the primary is a known late-type, H-rich Wolf-Rayet star (WN6h), the secondary has so far not been unambiguously detected. Using moderate-resolution spectra, we are able to derive accurate radial velocities for both components. By performing simultaneous orbital and polarimetric analyses, we derive the complete set of orbital parameters, including the inclination. The spectra are disentangled and spectroscopically analyzed, and an analysis of the wind-wind collision zone is conducted. The disentangled spectra and our models are consistent with a WN6h type for the primary and suggest that the secondary is an O3.5 If*/WN7 type star. We derive a high eccentricity of e = 0.78 and minimum masses of M1sin3I ≈ M2sin3I = 13 ± 2 M⊙, with q = M2/M1 = 1.01 ± 0.07. An analysis of emission excess stemming from a wind-wind collision yields an inclination similar to that obtained from polarimetry (I = 39 ± 6°). Our analysis thus implies and , excluding M1 > 300 M⊙. A detailed comparison with evolution tracks calculated for single and binary stars together with the high eccentricity suggests that the components of the system underwent quasi-homogeneous evolution and avoided mass-transfer. This scenario would suggest current masses of ≈ 80 M⊙ and initial masses of MI,1 ≈ 105 and MI,2 ≈ 90 M⊙, consistent with the upper limits of our derived orbital masses, and would imply an age of ≈ 2.2 Myr. A copy of the disentangled spectra, as either FITS files or tables are available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/598/A85

A Tank Bromeliad Favors Spider Presence in a Neotropical Inundated Forest

PubMed Central

Hénaut, Yann; Corbara, Bruno; Pélozuelo, Laurent; Azémar, Frédéric; Céréghino, Régis; Herault, Bruno; Dejean, Alain

2014-01-01

Tank bromeliads are good models for understanding how climate change may affect biotic associations. We studied the relationships between spiders, the epiphytic tank bromeliad, Aechmea bracteata, and its associated ants in an inundated forest in Quintana Roo, Mexico, during a drought period while, exceptionally, this forest was dry and then during the flooding that followed. We compared spider abundance and diversity between ‘Aechmea-areas’ and ‘control-areas’ of the same surface area. We recorded six spider families: the Dipluridae, Ctenidae, Salticidae, Araneidae, Tetragnathidae and Linyphiidae among which the funnel-web tarantula, Ischnothele caudata, the only Dipluridae noted, was the most abundant. During the drought period, the spiders were more numerous in the Aechmea-areas than in the control-areas, but they were not obligatorily associated with the Aechmea. During the subsequent flooding, the spiders were concentrated in the A. bracteata patches, particularly those sheltering an ant colony. Also, a kind of specificity existed between certain spider taxa and ant species, but varied between the drought period and subsequent flooding. We conclude that climatic events modulate the relationship between A. bracteata patches and their associated fauna. Tank bromeliads, previously considered only for their ecological importance in supplying food and water during drought, may also be considered refuges for spiders during flooding. More generally, tank bromeliads have an important role in preserving non-specialized fauna in inundated forests. PMID:25494055

Documenting the efficacy of virtual reality exposure with psychophysiological and information processing measures.

PubMed

Côté, Sophie; Bouchard, Stéphane

2005-09-01

Many outcome studies have been conducted to assess the efficacy of virtual reality in the treatment of specific phobias. However, most studies used self-report data. The addition of objective measures of arousal and information processing mechanisms would be a valuable contribution in order to validate the usefulness of virtual reality in the treatment of anxiety disorders. The goal of this study was to document the impact of virtual reality exposure (VRE) on cardiac response and automatic processing of threatening stimuli. Twenty-eight adults suffering from arachnophobia were assessed and received an exposure-based treatment using virtual reality. General outcome and specific processes measures included a battery of standardized questionnaires, a pictorial emotional Stroop task, a behavioral avoidance test and a measure of participants' inter-beat intervals (IBI) while they were looking at a live tarantula. Assessment was conducted before and after treatment. Repeated measures ANOVAs revealed that therapy had a positive impact on questionnaire data, as well as on the behavioral avoidance test. Analyses made on the pictorial Stroop task showed that information processing of spider-related stimuli changed after treatment, which also indicates therapeutic success. Psychophysiological data also showed a positive change after treatment, suggesting a decrease in anxiety. In sum, VRE led to significant therapeutic improvements on objective measures as well as on self-report instruments.

A Tank Bromeliad Favors Spider Presence in a Neotropical Inundated Forest.

PubMed

Hénaut, Yann; Corbara, Bruno; Pélozuelo, Laurent; Azémar, Frédéric; Céréghino, Régis; Herault, Bruno; Dejean, Alain

2014-01-01

Tank bromeliads are good models for understanding how climate change may affect biotic associations. We studied the relationships between spiders, the epiphytic tank bromeliad, Aechmea bracteata, and its associated ants in an inundated forest in Quintana Roo, Mexico, during a drought period while, exceptionally, this forest was dry and then during the flooding that followed. We compared spider abundance and diversity between 'Aechmea-areas' and 'control-areas' of the same surface area. We recorded six spider families: the Dipluridae, Ctenidae, Salticidae, Araneidae, Tetragnathidae and Linyphiidae among which the funnel-web tarantula, Ischnothele caudata, the only Dipluridae noted, was the most abundant. During the drought period, the spiders were more numerous in the Aechmea-areas than in the control-areas, but they were not obligatorily associated with the Aechmea. During the subsequent flooding, the spiders were concentrated in the A. bracteata patches, particularly those sheltering an ant colony. Also, a kind of specificity existed between certain spider taxa and ant species, but varied between the drought period and subsequent flooding. We conclude that climatic events modulate the relationship between A. bracteata patches and their associated fauna. Tank bromeliads, previously considered only for their ecological importance in supplying food and water during drought, may also be considered refuges for spiders during flooding. More generally, tank bromeliads have an important role in preserving non-specialized fauna in inundated forests.

Modulation of Kv4.2 channels by a peptide isolated from the venom of the giant bird-eating tarantula Theraphosa leblondi.

PubMed

Ebbinghaus, Jan; Legros, Christian; Nolting, Andreas; Guette, Catherine; Celerier, Marie-Louise; Pongs, Olaf; Bähring, Robert

2004-06-15

In order to find new peptide inhibitors for voltage-dependent potassium (Kv) channels, we examined the effects of venom from Theraphosa leblondi on Kv channel-mediated currents with the whole-cell patch-clamp technique. Both A-type currents in cultured hippocampal neurons and A-type currents recorded from HEK 293 cells transiently expressing recombinant Kv4.2 channels were selectively inhibited by T. leblondi venom. No venom activity was observed on recombinant Kv1.3, Kv1.4, Kv2.1 or Kv3.4 channels. We purified and sequenced three novel homologous peptides from this venom, which are related to previously identified Kv4 channel-specific peptide inhibitors and were named T. leblondi toxin (TLTx) 1, 2 and 3. The mode of action of TLTx1 on recombinant Kv4.2 channels was studied in more detail. TLTx1 inhibited Kv4.2-mediated currents with an IC50 of approximately 200 nM, and macroscopic current inactivation was slowed in the presence of TLTx1. Notably, TLTx1 also caused a shallower voltage dependence of Kv4.2 peak conductance and a shift of the activation midpoint to more positive potentials (DeltaV1/2 = +35 mV). TLTx1 caused a noticable slowing of Kv4.2 activation kinetics, and Kv4.2 deactivation kinetics were accelerated by TLTx1 as infered from Rb+ tail current measurements. Chimeric Kv2.1(4.2L3-4) channels, in which the linker region between S3 and S4 of the TLTx1-insensitive Kv2.1 channel was replaced by the corresponding Kv4.2 domain, were sensitive to TLTx1. Apparently, TLTx1 can act as a gating modifier of Kv4.2 channels. Copyright 2004 Elsevier Ltd.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Walborn, Nolan R.; Sana, Hugues; Sabbi, Elena, E-mail: walborn@stsci.edu, E-mail: hsana@stsci.edu, E-mail: sabbi@stsci.edu

Extremely broad emission wings at Hβ and Hα have been found in VLT-FLAMES Tarantula Survey data for five very luminous BA supergiants in or near 30 Doradus in the Large Magellanic Cloud. The profiles of both lines are extremely asymmetrical, which we have found to be caused by very broad diffuse interstellar bands (DIBs) in the longward wing of Hβ and the shortward wing of Hα. These DIBs are well known to interstellar but not to many stellar specialists, so that the asymmetries may be mistaken for intrinsic features. The broad emission wings are generally ascribed to electron scattering, althoughmore » we note difficulties for that interpretation in some objects. Such profiles are known in some Galactic hyper/supergiants and are also seen in both active and quiescent Luminous Blue Variables (LBVs). No prior or current LBV activity is known in these 30 Dor stars, although a generic relationship to LBVs is not excluded; subject to further observational and theoretical investigation, it is possible that these very luminous supergiants are approaching the LBV stage for the first time. Their locations in the HRD and presumed evolutionary tracks are consistent with that possibility. The available evidence for spectroscopic variations of these objects is reviewed, while recent photometric monitoring does not reveal variability. A search for circumstellar nebulae has been conducted, with an indeterminate result for one of them.« less

Sky island diversification meets the multispecies coalescent - divergence in the spruce-fir moss spider (Microhexura montivaga, Araneae, Mygalomorphae) on the highest peaks of southern Appalachia.

PubMed

Hedin, Marshal; Carlson, Dave; Coyle, Fred

2015-07-01

Microhexura montivaga is a miniature tarantula-like spider endemic to the highest peaks of the southern Appalachian mountains and is known only from six allopatric, highly disjunct montane populations. Because of severe declines in spruce-fir forest in the late 20th century, M. montivaga was formally listed as a US federally endangered species in 1995. Using DNA sequence data from one mitochondrial and seven nuclear genes, patterns of multigenic genetic divergence were assessed for six montane populations. Independent mitochondrial and nuclear discovery analyses reveal obvious genetic fragmentation both within and among montane populations, with five to seven primary genetic lineages recovered. Multispecies coalescent validation analyses [guide tree and unguided Bayesian Phylogenetics and Phylogeography (BPP), Bayes factor delimitation (BFD)] using nuclear-only data congruently recover six or seven distinct lineages; BFD analyses using combined nuclear plus mitochondrial data favour seven or eight lineages. In stark contrast to this clear genetic fragmentation, a survey of secondary sexual features for available males indicates morphological conservatism across montane populations. While it is certainly possible that morphologically cryptic speciation has occurred in this taxon, this system may alternatively represent a case where extreme population genetic structuring (but not speciation) leads to an oversplitting of lineage diversity by multispecies coalescent methods. Our results have clear conservation implications for this federally endangered taxon and illustrate a methodological issue expected to become more common as genomic-scale data sets are gathered for taxa found in naturally fragmented habitats. © 2015 John Wiley & Sons Ltd.

An Abrupt Transformation of Phobic Behavior After a Post-Retrieval Amnesic Agent.

PubMed

Soeter, Marieke; Kindt, Merel

2015-12-15

Although disrupting the process of memory reconsolidation has a great potential for clinical practice, the fear-amnesic effects are typically demonstrated through Pavlovian conditioning. Given that older and stronger memories are generally more resistant to change, we tested whether disrupting reconsolidation would also diminish fear in individuals who had developed a persistent spider fear outside the laboratory. Spider-fearful participants received a single dose of 40 mg of the noradrenergic β-blocker propranolol (n = 15), double-blind and placebo-controlled (n = 15), after a short 2-min exposure to a tarantula. To test whether memory reactivation was necessary to observe a fear-reducing effect, one additional group of spider-fearful participants (n = 15) received a single dose of 40 mg propranolol without memory reactivation. Disrupting reconsolidation of fear memory transformed avoidance behavior into approach behavior in a virtual binary fashion-an effect that persisted at least 1 year after treatment. Interestingly the β-adrenergic drug did initially not affect the self-declared fear of spiders but instead these reports followed the instant behavioral transformation several months later. Our findings are in sharp contrast with the currently pharmacological and cognitive behavioral treatments for anxiety and related disorders. The β-adrenergic blocker was only effective when the drug was administered upon memory reactivation, and a modification in cognitive representations was not necessary to observe a change in fear behavior. A new wave of treatments that pharmacologically target the synaptic plasticity underlying learning and memory seems to be within reach. Copyright © 2015 Society of Biological Psychiatry. Published by Elsevier Inc. All rights reserved.

Ultrastructural characterization of the hemocytes of Lasiodora sp. (Koch, 1850) (Araneae: Theraphosidae).

PubMed

Soares, Tatiana; Cavalcanti, Marília Gabriela Dos Santos; Ferreira, Felipe Roberto Borba; Cavalcanti, Maria do Socorro de Mendonça; Alves, Luiz Carlos; Brayner, Fábio André; Paiva, Patrícia Maria Guedes

2013-05-01

This paper is the first descriptive review of hemolymph cell types in the circulation of the tarantula spider Lasiodora sp. These animals are more long-lived than other arthropods, and may live for approximately twenty years. Such remarkable longevity may result from a highly successful immune system, which in turn is directly correlated with hemocyte function. Since the literature on the genus Lasiodora sp. is limited, the main goal of the present study was to identify the different cell types by optical and transmission microscope. Six hemocyte types were characterized and called prohemocyte, granulocyte type I, granulocyte type II, spherulocyte, oenocytoid and plasmatocyte. Prohemocytes presented a large nucleus, elongated granulocytes type I showed the nucleus with the same cell format, elliptical granulocytes type II showed the central nucleus of identical shape, spherulocytes exhibited the nucleus filling almost the whole cell, oval oenocytoids showed eccentric nucleus and less dense cytoplasm, and irregular plasmatocytes showed a nucleus and no granules in cytoplasm. These polymorphic granulocytes presented a round, elongated, elliptical, oval or irregular profile with large and varied numbers of granules, except for plasmatocytes, that were agranular. Different densities and different concentrations of these granules were found at the periphery of the cell. The possible reasons and implications of differences and similarities between arthropods hemocytes are discussed. It can be concluded that there are six cell types in Lasiodora sp. This study is of the first step in the elucidation of the role these cells play in the circulatory and immune system in spiders. Copyright © 2013 Elsevier Ltd. All rights reserved.

A Method for 3D-Reconstruction of a Muscle Thick Filament Using the Tilt Series Images of a Single Filament Electron Tomogram

PubMed Central

Márquez, G.; Pinto, A.; Alamo, L.; Baumann, B.; Ye, F.; Winkler, H.; Taylor, K.; Padrón, R.

2014-01-01

Summary Myosin interacting-heads (MIH) motifs are visualized in 3D-reconstructions of thick filaments from striated muscle. These reconstructions are calculated by averaging methods using images from electron micrographs of grids prepared using numerous filament preparations. Here we propose an alternative method to calculate the 3D-reconstruction of a single thick filament using only a tilt series images recorded by electron tomography. Relaxed thick filaments, prepared from tarantula leg muscle homogenates, were negatively stained. Single-axis tilt series of single isolated thick filaments were obtained with the electron microscope at a low electron dose, and recorded on a CCD camera by electron tomography. An IHRSR 3D-recontruction was calculated from the tilt series images of a single thick filament. The reconstruction was enhanced by including in the search stage dual tilt image segments while only single tilt along the filament axis is usually used, as well as applying a band pass filter just before the back projection. The reconstruction from a single filament has a 40 Å resolution and clearly shows the presence of MIH motifs. In contrast, the electron tomogram 3D-reconstruction of the same thick filament –calculated without any image averaging and/or imposition of helical symmetry- only reveals MIH motifs infrequently. This is –to our knowledge- the first application of the IHRSR method to calculate a 3D reconstruction from tilt series images. This single filament IHRSR reconstruction method (SF-IHRSR) should provide a new tool to assess structural differences between well-ordered thick (or thin) filaments in a grid by recording separately their electron tomograms. PMID:24727133

A method for 3D-reconstruction of a muscle thick filament using the tilt series images of a single filament electron tomogram.

PubMed

Márquez, G; Pinto, A; Alamo, L; Baumann, B; Ye, F; Winkler, H; Taylor, K; Padrón, R

2014-05-01

Myosin interacting-heads (MIH) motifs are visualized in 3D-reconstructions of thick filaments from striated muscle. These reconstructions are calculated by averaging methods using images from electron micrographs of grids prepared using numerous filament preparations. Here we propose an alternative method to calculate the 3D-reconstruction of a single thick filament using only a tilt series images recorded by electron tomography. Relaxed thick filaments, prepared from tarantula leg muscle homogenates, were negatively stained. Single-axis tilt series of single isolated thick filaments were obtained with the electron microscope at a low electron dose, and recorded on a CCD camera by electron tomography. An IHRSR 3D-recontruction was calculated from the tilt series images of a single thick filament. The reconstruction was enhanced by including in the search stage dual tilt image segments while only single tilt along the filament axis is usually used, as well as applying a band pass filter just before the back projection. The reconstruction from a single filament has a 40 Å resolution and clearly shows the presence of MIH motifs. In contrast, the electron tomogram 3D-reconstruction of the same thick filament - calculated without any image averaging and/or imposition of helical symmetry - only reveals MIH motifs infrequently. This is - to our knowledge - the first application of the IHRSR method to calculate a 3D reconstruction from tilt series images. This single filament IHRSR reconstruction method (SF-IHRSR) should provide a new tool to assess structural differences between well-ordered thick (or thin) filaments in a grid by recording separately their electron tomograms. Copyright © 2014 Elsevier Inc. All rights reserved.

Arachnids Secrete a Fluid over Their Adhesive Pads

PubMed Central

Peattie, Anne M.; Dirks, Jan-Henning; Henriques, Sérgio; Federle, Walter

2011-01-01

Background Many arachnids possess adhesive pads on their feet that help them climb smooth surfaces and capture prey. Spider and gecko adhesives have converged on a branched, hairy structure, which theoretically allows them to adhere solely by dry (solid-solid) intermolecular interactions. Indeed, the consensus in the literature is that spiders and their smooth-padded relatives, the solifugids, adhere without the aid of a secretion. Methodology and Principal Findings We investigated the adhesive contact zone of living spiders, solifugids and mites using interference reflection microscopy, which allows the detection of thin liquid films. Like insects, all the arachnids we studied left behind hydrophobic fluid footprints on glass (mean refractive index: 1.48–1.50; contact angle: 3.7–11.2°). Fluid was not always secreted continuously, suggesting that pads can function in both wet and dry modes. We measured the attachment forces of single adhesive setae from tarantulas (Grammostola rosea) by attaching them to a bending beam with a known spring constant and filming the resulting deflection. Individual spider setae showed a lower static friction at rest (26%±2.8 SE of the peak friction) than single gecko setae (Thecadactylus rapicauda; 96%±1.7 SE). This may be explained by the fact that spider setae continued to release fluid after isolation from the animal, lubricating the contact zone. Significance This finding implies that tarsal secretions occur within all major groups of terrestrial arthropods with adhesive pads. The presence of liquid in an adhesive contact zone has important consequences for attachment performance, improving adhesion to rough surfaces and introducing rate-dependent effects. Our results leave geckos and anoles as the only known representatives of truly dry adhesive pads in nature. Engineers seeking biological inspiration for synthetic adhesives should consider whether model species with fluid secretions are appropriate to their design goals

Analysis of the structural and molecular basis of voltage-sensitive sodium channel inhibition by the spider toxin huwentoxin-IV (μ-TRTX-Hh2a).

PubMed

Minassian, Natali A; Gibbs, Alan; Shih, Amy Y; Liu, Yi; Neff, Robert A; Sutton, Steven W; Mirzadegan, Tara; Connor, Judith; Fellows, Ross; Husovsky, Matthew; Nelson, Serena; Hunter, Michael J; Flinspach, Mack; Wickenden, Alan D

2013-08-02

Voltage-gated sodium channels (VGSCs) are essential to the normal function of the vertebrate nervous system. Aberrant function of VGSCs underlies a variety of disorders, including epilepsy, arrhythmia, and pain. A large number of animal toxins target these ion channels and may have significant therapeutic potential. Most of these toxins, however, have not been characterized in detail. Here, by combining patch clamp electrophysiology and radioligand binding studies with peptide mutagenesis, NMR structure determination, and molecular modeling, we have revealed key molecular determinants of the interaction between the tarantula toxin huwentoxin-IV and two VGSC isoforms, Nav1.7 and Nav1.2. Nine huwentoxin-IV residues (F6A, P11A, D14A, L22A, S25A, W30A, K32A, Y33A, and I35A) were important for block of Nav1.7 and Nav1.2. Importantly, molecular dynamics simulations and NMR studies indicated that folding was normal for several key mutants, suggesting that these amino acids probably make specific interactions with sodium channel residues. Additionally, we identified several amino acids (F6A, K18A, R26A, and K27A) that are involved in isoform-specific VGSC interactions. Our structural and functional data were used to model the docking of huwentoxin-IV into the domain II voltage sensor of Nav1.7. The model predicts that a hydrophobic patch composed of Trp-30 and Phe-6, along with the basic Lys-32 residue, docks into a groove formed by the Nav1.7 S1-S2 and S3-S4 loops. These results provide new insight into the structural and molecular basis of sodium channel block by huwentoxin-IV and may provide a basis for the rational design of toxin-based peptides with improved VGSC potency and/or selectivity.

Hubble:WFPC2 and ESO:2.2-m Composite Image of 30 Dor Runaway Star

NASA Image and Video Library

2017-12-08

NASA image release May 11, 2010 Hubble Catches Heavyweight Runaway Star Speeding from 30 Doradus Image: Hubble/WFPC2 and ESO/2.2-m Composite Image of 30 Dor Runaway Star A blue-hot star, 90 times more massive than our Sun, is hurtling across space fast enough to make a round trip from Earth to the Moon in merely two hours. Though the speed is not a record-breaker, it is unique to find a homeless star that has traveled so far from its nest. The only way the star could have been ejected from the star cluster where it was born is through a tussle with a rogue star that entered the binary system where the star lived, which ejected the star through a dynamical game of stellar pinball. This is strong circumstantial evidence for stars as massive as 150 times our Sun's mass living in the cluster. Only a very massive star would have the gravitational energy to eject something weighing 90 solar masses. The runaway star is on the outskirts of the 30 Doradus nebula, a raucous stellar breeding ground in the nearby Large Magellanic Cloud. The finding bolsters evidence that the most massive stars in the local universe reside in 30 Doradus, making it a unique laboratory for studying heavyweight stars. 30 Doradus, also called the Tarantula Nebula, is roughly 170,000 light-years from Earth. To learn more about this image go to: www.nasa.gov/mission_pages/hubble/science/runaway-star.html Credit: NASA, ESA, J. Walsh (ST-ECF), and ESO NASA Goddard Space Flight Center is home to the nation's largest organization of combined scientists, engineers and technologists that build spacecraft, instruments and new technology to study the Earth, the sun, our solar system, and the universe.

Next VLT Instrument Ready for the Astronomers

NASA Astrophysics Data System (ADS)

2000-02-01

adapted to the shape of the objects, thus increasing the scientific return. Results obtained so far look very promising. To increase further the scientific power of the FORS2 instrument in the spectroscopic mode, a number of new optical dispersion elements ("grisms", i.e., a combination of a grating and a glass prism) have been added. They give the scientists a greater choice of spectral resolution and wavelength range. Another mode that is new to FORS2 is the high time resolution mode. It was demonstrated with the Crab pulsar, cf. ESO PR 17/99 and promises very interesting scientific returns. Images from the FORS2 Commissioning Phase The two composite images shown below were obtained during the FORS2 commissioning work. They are based on three exposures through different optical broadband filtres (B: 429 nm central wavelength; 88 nm FWHM (Full Width at Half Maximum), V: 554/111 nm, R: 655/165 nm). All were taken with the 2048 x 2048 pixel 2 CCD detector with a field of view of 6.8 x 6.8 arcmin 2 ; each pixel measures 24 µm square. They were flatfield corrected and bias subtracted, scaled in intensity and some cosmetic cleaning was performed, e.g. removal of bad columns on the CCD. North is up and East is left. Tarantula Nebula in the Large Magellanic Cloud ESO Press Photo 05a/00 ESO Press Photo 05a/00 [Preview; JPEG: 400 x 452; 52k] [Normal; JPEG: 800 x 903; 142k] [Full-Res; JPEG: 2048 x 2311; 2.0Mb] The Tarantula Nebula in the Large Magellanic Cloud , as obtained with FORS2 at KUEYEN during the recent Commissioning period. It was taken during the night of January 31 - February 1, 2000. It is a composite of three exposures in B (30 sec exposure, image quality 0.75 arcsec; here rendered in blue colour), V (15 sec, 0.70 arcsec; green) and R (10 sec, 0.60 arcsec; red). The full-resolution version of this photo retains the orginal pixels. 30 Doradus , also known as the Tarantula Nebula , or NGC 2070 , is located in the Large Magellanic Cloud (LMC) , some 170,000 light

Drug Distribution into Peripheral Nerve.

PubMed

Liu, Houfu; Chen, Yan; Huang, Liang; Sun, Xueying; Fu, Tingting; Wu, Shengqian; Zhu, Xiaoyan; Zhen, Wei; Liu, Jihong; Lu, Gang; Cai, Wei; Yang, Ting; Zhang, Wandong; Yu, Xiaohong; Wan, Zehong; Wang, Jianfei; Summerfield, Scott G; Dong, Kelly; Terstappen, Georg C

2018-05-01

Little is known about the impact of the blood-nerve barrier (BNB) on drug distribution into peripheral nerves. In this study, we examined the peripheral nerve penetration in rats of 11 small-molecule drugs possessing diverse physicochemical and transport properties and ProTx-II, a tarantula venom peptide with molecular mass of 3826 Daltons. Each drug was administered as constant rate intravenous infusion for 6 hours (small molecules) or 24 hours (ProTx-II). Blood and tissues including brain, spinal cord, sciatic nerve, and dorsal root ganglion (DRG) were collected for drug concentration measurements. Unbound fractions of a set of compounds were determined by equilibrium dialysis method in rat blood, brains, spinal cords, sciatic nerves, and DRG. We also investigated the influence of N -[4-[2-(6,7-dimethoxy-3,4-dihydro-1 H -isoquinolin-2-yl)ethyl]phenyl]-5-methoxy-9-oxo-10 H -acridine-4-carboxamide (GF120918), a P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP) inhibitor, on the peripheral nerve and central nervous system (CNS) tissue penetration of imatinib. We found that: 1) the unbound fraction in brain tissue homogenate highly correlates with that in the spinal cord, sciatic nerve, and DRG for a set of compounds and thus provides a good surrogate for spinal cord and peripheral nerve tissues, 2) small-molecule drugs investigated can penetrate the DRG and sciatic nerve, 3) P-gp and BCRP have a limited impact on the distribution of small-molecule drugs into peripheral nerves, and 4) DRG is permeable to ProTx-II, but its distribution into sciatic nerve and CNS tissues is restricted. These results demonstrate that small-molecule drugs investigated can penetrate peripheral nerve tissues, and P-gp/BCRP may not be a limiting factor at the BNB. Biologics as large as ProTx-II can access the DRG but not sciatic nerve and CNS tissues. Copyright © 2018 by The American Society for Pharmacology and Experimental Therapeutics.

First results from SAM-FP: Fabry-Perot observations with ground-layer adaptive optics - the structure and kinematics of the core of 30 Doradus

NASA Astrophysics Data System (ADS)

Mendes de Oliveira, C.; Amram, P.; Quint, Bruno C.; Torres-Flores, S.; Barbá, R.; Andrade, D.

2017-08-01

The aim of this paper is to present the first data set obtained with SOAR Adaptive Module-Fabry-Parot (SAM-FP), a Fabry-Perot instrument mounted inside the SOAR telescope Adaptive-Optics Module. This is the only existing imaging Fabry-Perot interferometer using laser-assisted ground-layer adaptive optics. SAM-FP was used to observe the ionized gas, traced by Hα, in the centre of the 30 Doradus starburst (the Tarantula Nebula) in the Large Magellanic Cloud, with high spatial (˜0.6 arcsec, or 0.15 pc) and spectral (R ≃ 11 200) resolution. Radial velocity, velocity dispersion and monochromatic maps were derived. The region displays a mix of narrow, σ ˜ 20 km s-1 profiles and multiple broader profiles with σ ˜ 70-80 km s-1, indicating the complex nature of the nebula kinematics. A comparison with previously obtained VLT/FLAMES spectroscopy demonstrates that the data agree well in the regions of overlap, but the Fabry-Perot data are superior in spatial coverage. A preliminary analysis of the observations finds a new expanding bubble south of R136, with a projected radius of r = 5.6 pc and an expansion velocity of 29 ± 4 km s-1. In addition, the first-time detailed kinematic maps derived here for several complexes and filaments of 30 Doradus allow identification of kinematically independent structures. These data exemplify the power of the combination of a high-order Fabry-Perot with a wide-field imager (3 × 3 arcmin2 GLAO-corrected field of view) for high-resolution spatial and spectral studies. In particular, SAM-FP data cubes are highly advantageous over multifibre or long-slit data sets for nebula structure studies and to search for small-scale bubbles, given their greatly improved spatial coverage. For reference, this paper also presents two appendices with detailed descriptions of the usage of Fabry-Perot devices, including formulae and explanations for understanding Fabry-Perot observations.

ProTx-II, a selective inhibitor of NaV1.7 sodium channels, blocks action potential propagation in nociceptors.

PubMed

Schmalhofer, William A; Calhoun, Jeffrey; Burrows, Rachel; Bailey, Timothy; Kohler, Martin G; Weinglass, Adam B; Kaczorowski, Gregory J; Garcia, Maria L; Koltzenburg, Martin; Priest, Birgit T

2008-11-01

Voltage-gated sodium (Na(V)1) channels play a critical role in modulating the excitability of sensory neurons, and human genetic evidence points to Na(V)1.7 as an essential contributor to pain signaling. Human loss-of-function mutations in SCN9A, the gene encoding Na(V)1.7, cause channelopathy-associated indifference to pain (CIP), whereas gain-of-function mutations are associated with two inherited painful neuropathies. Although the human genetic data make Na(V)1.7 an attractive target for the development of analgesics, pharmacological proof-of-concept in experimental pain models requires Na(V)1.7-selective channel blockers. Here, we show that the tarantula venom peptide ProTx-II selectively interacts with Na(V)1.7 channels, inhibiting Na(V)1.7 with an IC(50) value of 0.3 nM, compared with IC(50) values of 30 to 150 nM for other heterologously expressed Na(V)1 subtypes. This subtype selectivity was abolished by a point mutation in DIIS3. It is interesting that application of ProTx-II to desheathed cutaneous nerves completely blocked the C-fiber compound action potential at concentrations that had little effect on Abeta-fiber conduction. ProTx-II application had little effect on action potential propagation of the intact nerve, which may explain why ProTx-II was not efficacious in rodent models of acute and inflammatory pain. Mono-iodo-ProTx-II ((125)I-ProTx-II) binds with high affinity (K(d) = 0.3 nM) to recombinant hNa(V)1.7 channels. Binding of (125)I-ProTx-II is insensitive to the presence of other well characterized Na(V)1 channel modulators, suggesting that ProTx-II binds to a novel site, which may be more conducive to conferring subtype selectivity than the site occupied by traditional local anesthetics and anticonvulsants. Thus, the (125)I-ProTx-II binding assay, described here, offers a new tool in the search for novel Na(V)1.7-selective blockers.

The Dancing Manias: Psychogenic Illness as a Social Phenomenon.

PubMed

Lanska, Douglas J

2018-01-01

The dancing mania erupted in the 14th century in the wake of the Black Death, and recurred for centuries in central Europe - particularly Germany, the Netherlands, and Belgium - finally abating in the early 17th century. The term "dancing mania" was derived from "choreomania," a concatenation of choros (dance) and mania (madness). A variant, tarantism, was prevalent in southern Italy from the 15th to the 17th centuries, and was attributed at the time to bites from the tarantula spider. Affected individuals participated in continuous, prolonged, erratic, often frenzied and sometimes erotic, dancing. In the 14th century, the dancing mania was linked to a corruption of the festival of St. John's Day by ancient pagan customs, but by the 16th century it was commonly considered an ordeal sent by a saint, or a punishment from God for people's sins. Consequently, during outbreaks in the 14th and 15th centuries, the dancing mania was considered an issue for magistrates and priests, not physicians, even though the disorder proved intractable to decrees and exorcisms. However, in the 16th century Paracelsus discounted the idea that the saints caused or interceded in the cure of the dancing mania; he instead suggested a psychogenic or malingered etiology, and this reformulation brought the dancing mania within the purview of physicians. Paracelsus advocated various mystical, psychological, and pharmacological approaches, depending on the presumptive etiologic factors with individual patients. Only music provided any relief for tarantism. Later authors suggested that the dancing mania was a mass stress-induced psychosis, a mass psychogenic illness, a culturally determined form of ritualized behavior, a manifestation of religious ecstasy, or even the result of food poisoning caused by the toxic and psychoactive chemical products of ergot fungi. In reality, dancing manias did not have a single cause, but component causes likely included psychogenic illness, malingering, and

Cold tolerance mechanisms of two arthropods from the Andean Range of Central Chile: Agathemera crassa (Insecta: Agathemeridae) and Euathlus condorito (Arachnida: Theraphosidae).

PubMed

Cubillos, Claudio; Cáceres, Juan Carlos; Villablanca, Cristopher; Villarreal, Pablo; Baeza, Marcelo; Cabrera, Ricardo; Graether, Steffen P; Veloso, Claudio

2018-05-01

Two strategies have been described for cold tolerance in arthropods: (1) freeze-tolerant organisms, which can survive the formation of ice crystals and (2) freeze-avoidant organisms, which prevent the ice crystal formation by super cooling their internal fluids. We studied two arthropods from the Andean Range in central Chile (2400 m a.s.l.), the stick insect Agathemera crassa commonly named as "Chinchemolle", and the tarantula spider Euathlus condorito commonly named as "Araña pollito", in order to evaluate how they respond to low temperatures at the physiological and molecular levels. We sampled the soil temperature during one year to track the temperature changes that these organisms must overcome. We found minimum temperatures around -6 °C in autumn, while the temperature were stable at 0 °C in winter due to the snow. The average field-cooling rate was 0.01 ± 0.006 °C min -1 . For both arthropods we determined the super cooling point (SCP) at a cooling rate of 1 °C min -1 and its subsequent survival, finding that A. crassa is a freezing tolerant organism with a SCP of -3.8 ± 1.8 °C and 100% survival, while E. condorito is a freezing avoidant organism with a SCP of -3.0 ± 1.3 °C and 0% survival. The SCP and survival were not affected by the season in which individuals were collected, the SCP was significantly affected by the cooling rate of the experiment. Both species had low molecular weight cryoprotective in their hemolymph that could explain their cold-tolerance behavior. Glucose, glycerol, and trehalose were found in A. crassa's hemolymph, only glucose and glycerol were found in E. condorito's. We analyzed the hemolymph proteins and found no seasonal differences in composition for either species and also we detected protein antifreeze activity in the hemolymph from both arthropods. Copyright © 2018 Elsevier Ltd. All rights reserved.

Expanding anchored hybrid enrichment to resolve both deep and shallow relationships within the spider tree of life.

PubMed

Hamilton, Chris A; Lemmon, Alan R; Lemmon, Emily Moriarty; Bond, Jason E

2016-10-13

Despite considerable effort, progress in spider molecular systematics has lagged behind many other comparable arthropod groups, thereby hindering family-level resolution, classification, and testing of important macroevolutionary hypotheses. Recently, alternative targeted sequence capture techniques have provided molecular systematics a powerful tool for resolving relationships across the Tree of Life. One of these approaches, Anchored Hybrid Enrichment (AHE), is designed to recover hundreds of unique orthologous loci from across the genome, for resolving both shallow and deep-scale evolutionary relationships within non-model systems. Herein we present a modification of the AHE approach that expands its use for application in spiders, with a particular emphasis on the infraorder Mygalomorphae. Our aim was to design a set of probes that effectively capture loci informative at a diversity of phylogenetic timescales. Following identification of putative arthropod-wide loci, we utilized homologous transcriptome sequences from 17 species across all spiders to identify exon boundaries. Conserved regions with variable flanking regions were then sought across the tick genome, three published araneomorph spider genomes, and raw genomic reads of two mygalomorph taxa. Following development of the 585 target loci in the Spider Probe Kit, we applied AHE across three taxonomic depths to evaluate performance: deep-level spider family relationships (33 taxa, 327 loci); family and generic relationships within the mygalomorph family Euctenizidae (25 taxa, 403 loci); and species relationships in the North American tarantula genus Aphonopelma (83 taxa, 581 loci). At the deepest level, all three major spider lineages (the Mesothelae, Mygalomorphae, and Araneomorphae) were supported with high bootstrap support. Strong support was also found throughout the Euctenizidae, including generic relationships within the family and species relationships within the genus Aptostichus. As in the

Hubble Sees Turquoise-Tinted Plumes in Large Magellanic Cloud

NASA Image and Video Library

2017-12-08

The brightly glowing plumes seen in this image are reminiscent of an underwater scene, with turquoise-tinted currents and nebulous strands reaching out into the surroundings. However, this is no ocean. This image actually shows part of the Large Magellanic Cloud (LMC), a small nearby galaxy that orbits our galaxy, the Milky Way, and appears as a blurred blob in our skies. The NASA/European Space Agency (ESA) Hubble Space Telescope has peeked many times into this galaxy, releasing stunning images of the whirling clouds of gas and sparkling stars (opo9944a, heic1301, potw1408a). This image shows part of the Tarantula Nebula's outskirts. This famously beautiful nebula, located within the LMC, is a frequent target for Hubble (heic1206, heic1402). In most images of the LMC the color is completely different to that seen here. This is because, in this new image, a different set of filters was used. The customary R filter, which selects the red light, was replaced by a filter letting through the near-infrared light. In traditional images, the hydrogen gas appears pink because it shines most brightly in the red. Here however, other less prominent emission lines dominate in the blue and green filters. This data is part of the Archival Pure Parallel Project (APPP), a project that gathered together and processed over 1,000 images taken using Hubble’s Wide Field Planetary Camera 2, obtained in parallel with other Hubble instruments. Much of the data in the project could be used to study a wide range of astronomical topics, including gravitational lensing and cosmic shear, exploring distant star-forming galaxies, supplementing observations in other wavelength ranges with optical data, and examining star populations from stellar heavyweights all the way down to solar-mass stars. Image Credit: ESA/Hubble & NASA: acknowledgement: Josh Barrington NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science

Hubble Unveils Colorful and Turbulent Star-Birth Region on 100,000th Orbit Milestone

NASA Technical Reports Server (NTRS)

2008-01-01

[figure removed for brevity, see original site] Click on the image for orientation annotation

In commemoration of NASA's Hubble Space Telescope completing its 100,000th orbit in its 18th year of exploration and discovery, scientists at the Space Telescope Science Institute in Baltimore, Md., have aimed Hubble totake a snapshot of a dazzling region of celestial birth and renewal.

Hubble peered into a small portion of the nebula near the star cluster NGC 2074 (upper, left). The region is a firestorm of raw stellar creation, perhaps triggered by a nearby supernova explosion. It lies about 170,000 light-years away near the Tarantula nebula, one of the most active star-forming regions in our Local Group of galaxies.

The three-dimensional-looking image reveals dramatic ridges and valleys of dust, serpent-head 'pillars of creation,' and gaseous filaments glowing fiercely under torrential ultraviolet radiation. The region is on the edge of a dark molecular cloud that is an incubator for the birth of new stars.

The high-energy radiation blazing out from clusters of hot young stars already born in NGC 2074 is sculpting the wall of the nebula by slowly eroding it away. Another young cluster may be hidden beneath a circle of brilliant blue gas at center, bottom.

In this approximately 100-light-year-wide fantasy-like landscape, dark towers of dust rise above a glowing wall of gases on the surface of the molecular cloud. The seahorse-shaped pillar at lower, right is approximately 20 light-years long, roughly four times the distance between our Sun and the nearest star, Alpha Centauri.

The region is in the Large Magellanic Cloud (LMC), a satellite of our Milky Way galaxy. It is a fascinating laboratory for observing star-formation regions and their evolution. Dwarf galaxies like the LMC are considered to be the primitive building blocks of larger galaxies.

This representative color image was taken on August 10, 2008, with Hubble's Wide

VizieR Online Data Catalog: VFTS. O-type stellar content of 30 Dor (Walborn+, 2014)

NASA Astrophysics Data System (ADS)

Walborn, N. R.; Sana, H.; Simon-Diaz, S.; Maiz Apellaniz, J.; Taylor, W. D.; Evans, C. J.; Markova, N.; Lennon, D. J.; de Koter, A.

2014-06-01

Detailed spectral classifications are presented for 352 O-B0 stars in the VLT-FLAMES Tarantula Survey ESO Large Programme, of which 213 O-type are judged of sufficiently high quality for further morphological analysis. Among them, six subcategories of special interest are distinguished. (1) Several new examples of the earliest spectral types O2-O3 have been found, while a previously known example has been determined to belong to the nitrogen-rich ON2 class. (2) A group of extremely rapidly rotating main-sequence objects has been isolated, including the largest vsini values known, the spatial and radial-velocity distributions of which suggest ejection from the two principal ionizing clusters NGC 2070 and NGC 2060. (3) Several new examples of the evolved, rapidly rotating Onfp class show similar evidence, although at least some of them are spectroscopic binaries. (4) No fewer than 48 members of the Vz category, hypothesized to be on or near the zero-age main sequence, are found in this sample; in contrast to the rapid rotators, they are strongly concentrated to the ionizing clusters and a newly recognized region of current and recent star formation to the north, supporting their interpretation as very young objects, as do their relatively faint absolute magnitudes. (5) A surprisingly large fraction of the main-sequence spectra belong to the recently recognized V((fc)) class, with CIII emission lines of similar strength to the usual NIII in V((f)) spectra, although a comparable number of the latter are also present, as well as six objects with very high-quality data but no trace of either emission feature, presenting new challenges to physical interpretations. (6) Two mid-O Vz and three late-O giant/supergiant spectra with morphologically enhanced nitrogen lines have been detected. Absolute visual magnitudes have been derived for each star with individual extinction laws, and composite Hertzsprung-Russell diagrams provide evidence of the multiple generations present

Massive stars and miniature robots: today's research and tomorrow's technologies

NASA Astrophysics Data System (ADS)

Taylor, William David

2013-03-01

This thesis documents the reduction of the VLT-FLAMES Tarantula Survey (VFTS) data set, whilst also describing the analysis for one of the serendipitous discoveries: the massive binary R139. This high-mass binary will provide an excellent future calibration point for stellar models, in part as it seems to defy certain expectations about its evolution. Out with the VFTS, a search for binary companions around a trio of B-type supergiants is presented. These stars are surrounded by nebulae that closely resemble the triple-ring structure associated with the poorly-understood SN1987A. Do these stars share a similar evolutionary fate? While strong evidence is found for periodic pulsations in one of the stars, there appears to be no indication of a short-period binary companion suggested in the literature. Gathering observations from a wide range of environments builds a fuller picture of massive stars, but the samples remain somewhat limited. The coming generation of extremely large telescopes will open new regions for studies like the VFTS. Fully utilising these remarkable telescopes will require many new technologies, and this thesis presents one such development project. For adaptive-optics corrected, multi-object instruments it will be necessary to position small pick-off mirrors in the telescope¿s focal plane to select the sub-fields on the sky. This could be most efficiently achieved if the mirrors were self-propelled, which has led to a miniature robot project called MAPS - the Micro Autonomous Positioning System. A number of robots have been built with a footprint of only 30 x 30mm. These wirelessly-controlled robots draw their power from the floor on which they operate and have shown the potential to be positioned to an accuracy of tens of microns. This thesis details much of the early design work and testing of the robots, and also the development of the camera imaging system used to determine the position of the robots. The MAPS project is ongoing and a

Use of high-resolution imagery acquired from an unmanned aircraft system for fluvial mapping and estimating water-surface velocity in rivers

NASA Astrophysics Data System (ADS)

Kinzel, P. J.; Bauer, M.; Feller, M.; Holmquist-Johnson, C.; Preston, T.

2013-12-01

The use of unmanned aircraft systems (UAS) for environmental monitoring in the United States is anticipated to increase in the coming years as the Federal Aviation Administration (FAA) further develops guidelines to permit their integration into the National Airspace System. The U.S. Geological Survey's (USGS) National Unmanned Aircraft Systems Project Office routinely obtains Certificates of Authorization from the FAA for utilizing UAS technology for a variety of natural resource applications for the U.S. Department of the Interior (DOI). We evaluated the use of a small UAS along two reaches of the Platte River near Overton Nebraska, USA, to determine the accuracy of the system for mapping the extent and elevation of emergent sandbars and to test the ability of a hovering UAS to identify and track tracers to estimate water-surface velocity. The UAS used in our study is the Honeywell Tarantula Hawk RQ16 (T-Hawk), developed for the U.S. Army as a reconnaissance and surveillance platform. The T-Hawk has been recently modified by USGS, and certified for airworthiness by the DOI - Office of Aviation Services, to accommodate a higher-resolution imaging payload than was originally deployed with the system. The T-Hawk is currently outfitted with a Canon PowerShot SX230 HS with a 12.1 megapixel resolution and intervalometer to record images at a user defined time step. To increase the accuracy of photogrammetric products, orthoimagery and DEMs using structure-from-motion (SFM) software, we utilized ground control points in the study reaches and acquired imagery using flight lines at various altitudes (200-400 feet above ground level) and oriented both parallel and perpendicular to the river. Our results show that the mean error in the elevations derived from SFM in the upstream reach was 17 centimeters and horizontal accuracy was 6 centimeters when compared to 4 randomly distributed targets surveyed on emergent sandbars. In addition to the targets, multiple transects were

Two Galaxies for a Unique Event

NASA Astrophysics Data System (ADS)

2009-04-01

To celebrate the 100 Hours of Astronomy, ESO is sharing two stunning images of unusual galaxies, both belonging to the Sculptor group of galaxies. The images, obtained at two of ESO's observatories at La Silla and Paranal in Chile, illustrate the beauty of astronomy. ESO PR Photo 14a/09 Irregular Galaxy NGC 55 ESO PR Photo 14b/09 Spiral Galaxy NGC 7793 As part of the International Year of Astronomy 2009 Cornerstone project, 100 Hours of Astronomy, the ambitious "Around the World in 80 Telescopes" event is a unique live webcast over 24 hours, following night and day around the globe to some of the most advanced observatories on and off the planet. To provide a long-lasting memory of this amazing world tour, observatories worldwide are revealing wonderful, and previously unseen, astronomical images. For its part, ESO is releasing outstanding pictures of two galaxies, observed with telescopes at the La Silla and Paranal observatories. The first of these depicts the irregular galaxy NGC 55, a member of the prominent Sculptor group of galaxies in the southern constellation of Sculptor. The galaxy is about 70 000 light-years across, that is, a little bit smaller than our own Milky Way. NGC 55 actually resembles more our galactic neighbour, the Large Magellanic Cloud (LMC), although the LMC is seen face-on, whilst NGC 55 is edge-on. By studying about 20 planetary nebulae in this image, a team of astronomers found that NGC 55 is located about 7.5 million light-years away. They also found that the galaxy might be forming a bound pair with the gorgeous spiral galaxy NGC 300 . Planetary nebulae are the final blooming of Sun-like stars before their retirement as white dwarfs. This striking image of NGC 55, obtained with the Wide Field Imager on the 2.2-metre MPG/ESO telescope at La Silla, is dusted with a flurry of reddish nebulae, created by young, hot massive stars. Some of the more extended ones are not unlike those seen in the LMC, such as the Tarantula Nebula. The quality

Nitrogen line spectroscopy of O-stars. II. Surface nitrogen abundances for O-stars in the Large Magellanic Cloud

NASA Astrophysics Data System (ADS)

Rivero González, J. G.; Puls, J.; Najarro, F.; Brott, I.

2012-01-01

derive consistent nitrogen abundances. The bulk of our sample O-stars seems to be strongly nitrogen-enriched, and a clear correlation of nitrogen and helium enrichment is found. By comparing the nitrogen abundances as a function of v sin i ("Hunter-plot") with tailored evolutionary calculations, we identify a considerable number of highly enriched objects at low rotation. Conclusions: Our findings seem to support the basic outcome of previous B-star studies within the VLT-FLAMES survey. Owing to the low initial abundance, the detection of strong nitrogen enrichment in the bulk of O-stars indicates that efficient mixing takes place already during the very early phases of stellar evolution of LMC O-stars. For tighter constraints, however, upcoming results from the VLT-FLAMES Tarantula survey need to be waited for, which will comprise a much higher number of O-stars that will be analyzed based on similar methods as presented here. Based on observations collected at the European Southern Observatory Very Large Telescope, under programmes 68.D-0369, 171.D-0237 (FLAMES) and 67.D-0238, 70.D-0164, 074.D-0109 (UVES).Appendices A-C are available in electronic form at http://www.aanda.org

Lots of Small Stars Born in Starburst Region

NASA Astrophysics Data System (ADS)

1999-10-01

will quickly affect their environment, but how much? At this moment, nobody knows for sure what determines the actual masses of individual stars that are formed in a very massive and turbulent gas cloud, although some ideas can now be tested with these new observations. The NGC 3603 region The new VLT observations are the key part of a larger research programme that also includes observations of the stellar cluster in the famous Tarantula Nebula in the Large Magellanic Cloud (LMC) with the NICMOS instrument on the Hubble Space Telescope (HST), as well as adaptive optics observations with ground-based telescopes of more quiescent, star-forming regions in the Galaxy. However, the team considered the starburst region NGC 3603 as the best target for this kind of investigation. It is situated in the far southern constellation Carina (The Keel) and can only be observed from the South. Moreover, such a study has to focus on the densest part of the cluster that can only be resolved with a very sensitive infrared (IR) instrument under the best seeing conditions. The VLT ANTU telescope and the multi-mode ISAAC facility are ideally suited for this purpose. NGC 3603 is located in the Carina spiral arm in the Milky Way galaxy at a distance of about 20,000 light-years (6 - 7 kpc). It is the only massive, galactic "HII-region" (so denoted by astronomers because part of its hydrogen is ionized) in which a central cluster of strongly UV-radiating stars of types "O" and "B" that ionize the nebula can be studied at visual and near-infrared wavelengths. This is because the line-of-sight is reasonably free of dust in this direction; the dimming in near-infrared radiation due to intervening matter between the nebula and us is only about a factor of 2 (contrary to 80 in visible light). The total mass of the hot O- and B-stars in NGC 3603 is over 2000 solar masses. Together, the more than fifty heavy and bright O-stars in NGC 3603 have about 100 times the ionizing power of the well

ESO PR Highlights in 2006

NASA Astrophysics Data System (ADS)

2007-01-01

more energetic gamma-ray bursts. But not all the explosions are associated with supernovae, and a new kind of explosion is indeed suggested by the observation of a new mysterious category of gamma-ray bursts (PR 49/06). The Atacama Pathfinder Experiment (APEX) 12-m sub-millimetre telescope lived up to the ambitions of the scientists by providing access to the 'Cold Universe' with unprecedented sensitivity and image quality. As a demonstration, no less than 26 articles based on early science with APEX were published in a special issue of the research journal Astronomy & Astrophysics (PR 24/06). This year ESO and Chile celebrated ten years of collaboration: a cooperation that led not only to breakthrough discoveries, but also to a growth of astronomy and related sciences in the South American country (PR 21/06). ESO published many images last year as well, including two huge ones, made with the Wide Field Imager: one, made of about 300 million pixels, shows an 'empty field' (PR 14/06), while the other, a 256 million pixel mosaic, depicts in amazing detail the Tarantula Nebula (PR 50/06). These and other images can be accessed through the clickable map, including amazing images of galaxies and of a finally identified flying object (PR 48/06).

Stars Just Got Bigger - A 300 Solar Mass Star Uncovered

NASA Astrophysics Data System (ADS)

2010-07-01

Using a combination of instruments on ESO's Very Large Telescope, astronomers have discovered the most massive stars to date, one weighing at birth more than 300 times the mass of the Sun, or twice as much as the currently accepted limit of 150 solar masses. The existence of these monsters - millions of times more luminous than the Sun, losing weight through very powerful winds - may provide an answer to the question "how massive can stars be?" A team of astronomers led by Paul Crowther, Professor of Astrophysics at the University of Sheffield, has used ESO's Very Large Telescope (VLT), as well as archival data from the NASA/ESA Hubble Space Telescope, to study two young clusters of stars, NGC 3603 and RMC 136a in detail. NGC 3603 is a cosmic factory where stars form frantically from the nebula's extended clouds of gas and dust, located 22 000 light-years away from the Sun (eso1005). RMC 136a (more often known as R136) is another cluster of young, massive and hot stars, which is located inside the Tarantula Nebula, in one of our neighbouring galaxies, the Large Magellanic Cloud, 165 000 light-years away (eso0613). The team found several stars with surface temperatures over 40 000 degrees, more than seven times hotter than our Sun, and a few tens of times larger and several million times brighter. Comparisons with models imply that several of these stars were born with masses in excess of 150 solar masses. The star R136a1, found in the R136 cluster, is the most massive star ever found, with a current mass of about 265 solar masses and with a birthweight of as much as 320 times that of the Sun. In NGC 3603, the astronomers could also directly measure the masses of two stars that belong to a double star system [1], as a validation of the models used. The stars A1, B and C in this cluster have estimated masses at birth above or close to 150 solar masses. Very massive stars produce very powerful outflows. "Unlike humans, these stars are born heavy and lose weight as

Eye-openers from XMM-Newton

NASA Astrophysics Data System (ADS)

2000-02-01

The images were obtained between 19-25 January at the very start of the science payload commissioning process. The spacecraft viewed three regions of the sky: part of the Large Magellanic Cloud (LMC), the Hickson Cluster Group 16 (HCG-16), and the star HR 1099. These targets were chosen because they all present a variety of X-ray extended and point sources and are very interesting regions. The expanding cauldon in the Large Magellanic Cloud The Large Magellanic Cloud also known as the Nebula Major is about 20 thousand light years in diameter. Situated 160 thousand light years from Earth, it is one of two irregularly shaped galaxies that are easily seen with the naked eye in the southern hemisphere. These galaxies are satellites of the Milky Way and appear to be slowly spiralling into our own Galaxy. The first image obtained by the EPIC-PN X-ray cameras viewed the 30 Doradus region of the Large Magellanic Cloud. Also called the Tarantula Nebula, 30 Doradus is a cauldron of creation where exploding stars are releasing vast amounts of matter and where new stars are being born. The image presents the million degree temperatures of the emitting medium, with blue indicating the hottest regions; green intermediate temperatures and red the coldest regions. The white and blue arc-like formation just off the centre is a new object, only part of which was known in the past. It has the appearance of a supernova remnant with its expanding glowing-hot gas producing X-ray emission as it collides with the interstellar medium. On the lower right of the picture is the remains of a star that exploded as Supernova 1987A on 24 February 1987. It was the first supernova to reach naked-eye brightness since 1604 (Kepler's star) and remained visible to the naked eye for nearly nine months. The brightest source in the view, upper left, is another supernova remnant (N157D). Martin Turner, Principal Investigator for the EPIC cameras... "These first pictures are tremendously exciting after so

A Portrait of One Hundred Thousand and One Galaxies

NASA Astrophysics Data System (ADS)

2002-08-01

about 2000 light-years in diameter, thus dwarfing even the enormous Tarantula Nebula in the LMC, also photographed with the WFI (cf. ESO PR Photos 14a-g/02 ). The largest versions ("normal" or "full-res") of this and the following photos are shown with their original pixel size, demonstrating the incredible amount of detail visible on one WFI image. Technical information about this photo is available below. In 1999, Wolfgang Gieren (Universidad de Concepcion, Chile) and his colleagues started a search for Cepheid-type variable stars in NGC 300. These stars constitute a key element in the measurement of distances in the Universe. It has been known since many years that the pulsation period of a Cepheid-type star depends on its intrinsic brightness (its "luminosity"). Thus, once its period has been measured, the astronomers can calculate its luminosity. By comparing this to the star's apparent brightness in the sky, and applying the well-known diminution of light with the second power of the distance, they can obtain the distance to the star. This fundamental method has allowed some of the most reliable measurements of distances in the Universe and has been essential for all kinds of astrophysics, from the closest stars to the remotest galaxies. Previous to Gieren's new project, only about a dozen Cepheids were known in NGC 300. However, by regularly obtaining wide-field WFI exposures of NGC 300 from July 1999 through January 2000 and carefully monitoring the apparent brightness of its brighter stars during that period, the astronomers detected more than 100 additional Cepheids . The brightness variations (in astronomical terminology: "light curves") could be determined with excellent precision from the WFI data. They showed that the pulsation periods of these Cepheids range from about 5 to 115 days. Some of these Cepheids are identified on PR Photo 18b/02 , in the middle of a very crowded field in NGC 300. When fully studied, these unique observational data will yield a