Morphometric variability of Arctodiaptomus salinus (Copepoda) in the Mediterranean-Black Sea region

PubMed Central

ANUFRIIEVA, Elena V.; SHADRIN, Nickolai V.

2015-01-01

Inter-species variability in morphological traits creates a need to know the range of variability of characteristics in the species for taxonomic and ecological tasks. Copepoda Arctodiaptomus salinus, which inhabits water bodies across Eurasia and North Africa, plays a dominant role in plankton of different water bodies-from fresh to hypersaline. This work assesses the intra- and inter-population morphometric variability of A. salinus in the Mediterranean-Black Sea region and discusses some observed regularities. The variability of linear body parameters and proportions was studied. The impacts of salinity, temperature, and population density on morphological characteristics and their variability can manifest themselves in different ways at the intra- and inter-population levels. A significant effect of salinity, pH and temperature on the body proportions was not found. Their intra-population variability is dependent on temperature and salinity. Sexual dimorphism of A. salinus manifests in different linear parameters, proportions, and their variability. There were no effects of temperature, pH and salinity on the female/male parameter ratio. There were significant differences in the body proportions of males and females in different populations. The influence of temperature, salinity, and population density can be attributed to 80%-90% of intra-population variability of A. salinus. However, these factors can explain less than 40% of inter-population differences. Significant differences in the body proportions of males and females from different populations may suggest that some local populations of A. salinus in the Mediterranean-Black Sea region are in the initial stages of differentiation. PMID:26646569

Experimental evidence for carbonate precipitation and CO 2 degassing during sea ice formation

NASA Astrophysics Data System (ADS)

Papadimitriou, S.; Kennedy, H.; Kattner, G.; Dieckmann, G. S.; Thomas, D. N.

2004-04-01

Chemical and stable carbon isotopic modifications during the freezing of artificial seawater were measured in four 4 m 3 tank incubations. Three of the four incubations were inoculated with a nonaxenic Antarctic diatom culture. The 18 days of freezing resulted in 25 to 27 cm thick ice sheets overlying the residual seawater. The ice phase was characterized by a decrease in temperature from -1.9 to -2.2°C in the under-ice seawater down to -6.7°C in the upper 4 cm of the ice sheet, with a concurrent increase in the salinity of the under-ice seawater and brine inclusions of the ice sheet as a result of physical concentration of major dissolved salts by expulsion from the solid ice matrix. Measurements of pH, total dissolved inorganic carbon (C T) and its stable isotopic composition (δ 13C T) all exhibited changes, which suggest minimal effect by biological activity during the experiment. A systematic drop in pH and salinity-normalized C T by up to 0.37 pH SWS units and 376 μmol C kg -1 respectively at the lowest temperature and highest salinity part of the ice sheet were coupled with an equally systematic 13C enrichment of the C T. Calculations based on the direct pH and C T measurements indicated a steady increase in the in situ concentration of dissolved carbon dioxide (CO 2(aq)) with time and increasing salinity within the ice sheet, partly due to changes in the dissociation constants of carbonic acid in the low temperature-high salinity range within sea ice. The combined effects of temperature and salinity on the solubility of CO 2 over the range of conditions encountered during this study was a slight net decrease in the equilibrium CO 2(aq) concentration as a result of the salting-out overriding the increase in solubility with decreasing temperature. Hence, the increase in the in situ CO 2(aq) concentration lead to saturation or supersaturation of the brine inclusions in the ice sheet with respect to atmospheric pCO 2 (≈3.5 × 10 -4 atm). When all physico-chemical processes are considered, we expect CO 2 degassing and carbonate mineral precipitation from the brine inclusions of the ice sheet, which were saturated or highly supersaturated with respect to both the anhydrous (calcite, aragonite, vaterite) and hydrated (ikaite) carbonate minerals.

Cryoelectrolysis—electrolytic processes in a frozen physiological saline medium

PubMed Central

Lugnani, Franco; Macchioro, Matteo

2017-01-01

Background Cryoelectrolysis is a new minimally invasive tissue ablation surgical technique that combines the ablation techniques of electrolytic ablation with cryosurgery. The goal of this study is to examine the hypothesis that electrolysis can take place in a frozen aqueous saline solution. Method To examine the hypothesis we performed a cryoelectrolytic ablation protocol in which electrolysis and cryosurgery are delivered simultaneously in a tissue simulant made of physiological saline gel with a pH dye. We measured current flow, voltage and extents of freezing and pH dye staining. Results Using optical measurements and measurements of currents, we have shown that electrolysis can occur in frozen physiological saline, at high subzero freezing temperatures, above the eutectic temperature of the frozen salt solution. It was observed that electrolysis occurs when the tissue resides at high subzero temperatures during the freezing stage and essentially throughout the entire thawing stage. We also found that during thawing, the frozen lesion temperature raises rapidly to high subfreezing values and remains at those values throughout the thawing stage. Substantial electrolysis occurs during the thawing stage. Another interesting finding is that electro-osmotic flows affect the process of cryoelectrolysis at the anode and cathode, in different ways. Discussion The results showing that electrical current flow and electrolysis occur in frozen saline solutions imply a mechanism involving ionic movement in the fluid concentrated saline solution channels between ice crystals, at high subfreezing temperatures. Temperatures higher than the eutectic are required for the brine to be fluid. The particular pattern of temperature and electrical currents during the thawing stage of frozen tissue, can be explained by the large amounts of energy that must be removed at the outer edge of the frozen lesion because of the solid/liquid phase transformation on that interface. Conclusion Electrolysis can occur in a frozen domain at high subfreezing temperature, probably above the eutectic. It appears that the most effective period for delivering electrolytic currents in cryoelectrolysis is during the high subzero temperatures stage while freezing and immediately after cooling has stopped, throughout the thawing stage. PMID:28123904

Cryoelectrolysis-electrolytic processes in a frozen physiological saline medium.

PubMed

Lugnani, Franco; Macchioro, Matteo; Rubinsky, Boris

2017-01-01

Cryoelectrolysis is a new minimally invasive tissue ablation surgical technique that combines the ablation techniques of electrolytic ablation with cryosurgery. The goal of this study is to examine the hypothesis that electrolysis can take place in a frozen aqueous saline solution. To examine the hypothesis we performed a cryoelectrolytic ablation protocol in which electrolysis and cryosurgery are delivered simultaneously in a tissue simulant made of physiological saline gel with a pH dye. We measured current flow, voltage and extents of freezing and pH dye staining. Using optical measurements and measurements of currents, we have shown that electrolysis can occur in frozen physiological saline, at high subzero freezing temperatures, above the eutectic temperature of the frozen salt solution. It was observed that electrolysis occurs when the tissue resides at high subzero temperatures during the freezing stage and essentially throughout the entire thawing stage. We also found that during thawing, the frozen lesion temperature raises rapidly to high subfreezing values and remains at those values throughout the thawing stage. Substantial electrolysis occurs during the thawing stage. Another interesting finding is that electro-osmotic flows affect the process of cryoelectrolysis at the anode and cathode, in different ways. The results showing that electrical current flow and electrolysis occur in frozen saline solutions imply a mechanism involving ionic movement in the fluid concentrated saline solution channels between ice crystals, at high subfreezing temperatures. Temperatures higher than the eutectic are required for the brine to be fluid. The particular pattern of temperature and electrical currents during the thawing stage of frozen tissue, can be explained by the large amounts of energy that must be removed at the outer edge of the frozen lesion because of the solid/liquid phase transformation on that interface. Electrolysis can occur in a frozen domain at high subfreezing temperature, probably above the eutectic. It appears that the most effective period for delivering electrolytic currents in cryoelectrolysis is during the high subzero temperatures stage while freezing and immediately after cooling has stopped, throughout the thawing stage.

Life under Multiple Extreme Conditions: Diversity and Physiology of the Halophilic Alkalithermophiles

PubMed Central

Wiegel, Juergen

2012-01-01

Around the world, there are numerous alkaline, hypersaline environments that are heated either geothermally or through intense solar radiation. It was once thought that such harsh environments were inhospitable and incapable of supporting a variety of life. However, numerous culture-dependent and -independent studies revealed the presence of an extensive diversity of aerobic and anaerobic bacteria and archaea that survive and grow under these multiple harsh conditions. This diversity includes the halophilic alkalithermophiles, a novel group of polyextremophiles that require for growth and proliferation the multiple extremes of high salinity, alkaline pH, and elevated temperature. Life under these conditions undoubtedly involves the development of unique physiological characteristics, phenotypic properties, and adaptive mechanisms that enable control of membrane permeability, control of intracellular osmotic balance, and stability of the cell wall, intracellular proteins, and other cellular constituents. This minireview highlights the ecology and growth characteristics of the extremely halophilic alkalithermophiles that have been isolated thus far. Biochemical, metabolic, and physiological properties of the extremely halophilic alkalithermophiles are described, and their roles in resistance to the combined stressors of high salinity, alkaline pH, and high temperature are discussed. The isolation of halophilic alkalithermophiles broadens the physicochemical boundaries for life and extends the boundaries for the combinations of the maximum salinity, pH, and temperature that can support microbial growth. PMID:22492435

Ecophysiology of Nais elinguis (Oligochaeta) in a brackish-water lagoon

NASA Astrophysics Data System (ADS)

Little, Colin

1984-02-01

Population densities of Nais elinguis Müller were determined in Swanpool, a brackish-water lagoon at Falmouth, Cornwall, U.K., over a four-year period. High densities were found only from January to May, usually with a peak in March. Significant negative correlations were shown between population density and both salinity and temperature. In laboratory tests, feeding rates remained unchanged from freshwater to 20‰ salinity (S), but declined above this salinity. Nais elinguis was shown to be a good osmoregulator, remaining hyperosmotic below 7‰ S, and hypo-osmotic above this. Feeding rate showed a Q 10 of approximately 2 from 1 to 25°C, but above this the rate declined. Feeding rate was unaffected between pH 6 and 11. Increased salinity to (10‰ S) did not influence the effect of temperature on feeding rate. This high salinity did reduce feeding rate at a pH of 10 and above. It is concluded that the physical and chemical variables considered are unlikely to be direct causal factors limiting populations of N. elinguis in Swanpool. The influence of food supply, competition, predation and changes in reproductive mode are discussed as possible controlling factors. It is shown that the population decline of N. elinguis in early summer usually coincides with the rise of populations of chironomid larvae.

Measuring pH variability using an experimental sensor on an underwater glider

NASA Astrophysics Data System (ADS)

Hemming, Michael P.; Kaiser, Jan; Heywood, Karen J.; Bakker, Dorothee C. E.; Boutin, Jacqueline; Shitashima, Kiminori; Lee, Gareth; Legge, Oliver; Onken, Reiner

2017-05-01

Autonomous underwater gliders offer the capability of measuring oceanic parameters continuously at high resolution in both vertical and horizontal planes, with timescales that can extend to many months. An experimental ion-sensitive field-effect transistor (ISFET) sensor measuring pH on the total scale was attached to a glider during the REP14-MED experiment in June 2014 in the Sardinian Sea in the northwestern Mediterranean. During the deployment, pH was sampled at depths of up to 1000 m along an 80 km transect over a period of 12 days. Water samples were collected from a nearby ship and analysed for dissolved inorganic carbon concentration and total alkalinity to derive the pH for validating the ISFET sensor measurements. The vertical resolution of the pH sensor was good (1 to 2 m), but stability was poor and the sensor drifted in a non-monotonous fashion. In order to remove the sensor drift, a depth-constant time-varying offset was applied throughout the water column for each dive, reducing the spread of the data by approximately two-thirds. Furthermore, the ISFET sensor required temperature- and pressure-based corrections, which were achieved using linear regression. Correcting for this decreased the apparent sensor pH variability by a further 13 to 31 %. Sunlight caused an apparent sensor pH decrease of up to 0.1 in surface waters around local noon, highlighting the importance of shielding the sensor from light in future deployments. The corrected pH from the ISFET sensor is presented along with potential temperature, salinity, potential density anomalies (σθ), and dissolved oxygen concentrations (c(O2)) measured by the glider, providing insights into the physical and biogeochemical variability in the Sardinian Sea. The pH maxima were identified close to the depth of the summer chlorophyll maximum, where high c(O2) values were also found. Longitudinal pH variations at depth (σθ > 28. 8 kg m-3) highlighted the variability of water masses in the Sardinian Sea. Higher pH was observed where salinity was > 38. 65, and lower pH was found where salinity ranged between 38.3 and 38.65. The higher pH was associated with saltier Levantine Intermediate Water, and it is possible that the lower pH was related to the remineralisation of organic matter. Furthermore, shoaling isopycnals closer to shore coinciding with low pH and c(O2), high salinity, alkalinity, dissolved inorganic carbon concentrations, and chlorophyll fluorescence waters may be indicative of upwelling.

Changes in wintertime pH and hydrography of the Gulf of Finland (Baltic Sea) with focus on depth layers.

PubMed

Almén, Anna-Karin; Glippa, Olivier; Pettersson, Heidi; Alenius, Pekka; Engström-Öst, Jonna

2017-04-01

We studied changes in sea water pH, temperature and salinity with focus on two depth layers, along the Gulf of Finland (the Baltic Sea) using long-term monitoring data from 1979 to 2015. Data from the most frequently sampled monitoring stations between western and eastern Gulf of Finland were used. The main result of the study reveals that pH has decreased both in surface and deep-water in the western Gulf of Finland with values ranging between -0.005 and -0.008 units year -1 . We also demonstrate a rise in temperature (~2 °C) and decrease in salinity (~-0.7 g kg -1 ) at several stations over the last 36 years. In general, the changes are shown to be more pronounced in the western part of the gulf. This paper also stresses the importance of improving the sampling frequency and quality of monitoring measurements.

Influence of water temperature and salinity on seasonal occurrences of Vibrio cholerae and enteric bacteria in oyster-producing areas of Veracruz, México.

PubMed

Castañeda Chávez, Maria del Refugio; Pardio Sedas, Violeta; Orrantia Borunda, Erasmo; Lango Reynoso, Fabiola

2005-12-01

The influence of temperature and salinity on the occurrence of Vibrio cholerae, Escherichia coli and Salmonella spp. associated with water and oyster samples was investigated in two lagoons on the Atlantic Coast of Veracruz, Mexico over a 1-year period. The results indicated that seasonal salinity variability and warm temperatures, as well as nutrient influx, may influence the occurrence of V. cholera. non-O1 and O1. The conditions found in the Alvarado (31.12 degrees C, 6.27 per thousand, pH=8.74) and La Mancha lagoons (31.38 degrees C, 24.18 per thousand, pH=9.15) during the rainy season 2002 favored the occurrence of V. cholera O1 Inaba enterotoxin positive traced in oysters. Vibrio alginolyticus was detected in Alvarado lagoon water samples during the winter season. E. coli and Salmonella spp. were isolated from water samples from the La Mancha (90-96.7% and 86.7-96.7%) and Alvarado (88.6-97.1% and 88.6-100%) lagoons. Occurrence of bacteria may be due to effluents from urban, agricultural and industrial areas.

Reduced Salinity Improves Marine Food Availability With Positive Feedbacks on pH in a Tidally-Dominated Estuary

NASA Astrophysics Data System (ADS)

Lowe, A. T.; Roberts, E. A.; Galloway, A. W. E.

2016-02-01

Coastal regions around the world are changing rapidly, generating many physiological stressors for marine organisms. Food availability, a major factor determining physiological condition of marine organisms, in these systems reflects the influence of biological and environmental factors, and will likely respond dramatically to long-term changes. Using observations of phytoplankton, detritus, and their corresponding fatty acids and stable isotopes of carbon, nitrogen and sulfur, we identified environmental drivers of pelagic food availability and quality along a salinity gradient in a large tidally influenced estuary (San Juan Archipelago, Salish Sea, USA). Variation in chlorophyll a (Chl a), biomarkers and environmental conditions exhibited a similar range at both tidal and seasonal scales, highlighting a tide-related mechanism controlling productivity that is important to consider for long-term monitoring. Multiple parameters of food availability were inversely and non-linearly correlated to salinity, such that availability of high-quality (based on abundance, essential fatty acid concentration and C:N) seston increased below a salinity threshold of 30. The increased marine productivity was associated with increased pH and dissolved oxygen (DO) at lower salinity. Based on this observation we predicted that a decrease of salinity to below the threshold would result in higher Chl a, temperature, DO and pH across a range of temporal and spatial scales, and tested the prediction with a meta-analysis of available data. At all scales, these variables showed significant and consistent increases related to the salinity threshold. This finding provides important context to the increased frequency of below-threshold salinity over the last 71 years in this region, suggesting greater food availability with positive feedbacks on DO and pH. Together, these findings indicate that many of the environmental factors predicted to increase physiological stress to benthic suspension feeders (e.g. decreased salinity) may simultaneously and paradoxically improve conditions for benthic organisms.

Production, characterization, and immobilization of partially purified surfactant-detergent and alkali-thermostable protease from newly isolated Aeromonas caviae.

PubMed

Datta, Sumitra; Menon, Gopalakrishnan; Varughese, Bincy

2017-04-21

Proteolytic Aeromonas caviae P-1-1 growing at wide-ranging pH (7.0-11.0) and moderate salinity (0-5% NaCl) was isolated from cattle shed of Thanjavur, India. It produced lipase, gelatinase, and polyhydroxybutyrate. Different culture conditions, incubation time, carbon and nitrogen sources, vitamins, amino acids, surfactants, and metal ions for optimal growth and protease production of P-1-1 were examined. Maximum protease (0.128 U/mL) production was achieved with 1% fructose, 1% yeast extract, 0.1% ammonium sulfate, 3% NaCl, 0.1% CaCl 2  · 2H 2 O, 1% glycine, 0.1% vitamin E, and 0.1% Tween-40 at pH 8.0 after 42 hr of incubation at 37°C. It was active over broad range of pH (7.0-12.0), temperature (15-100°C), and salinity (0-9% NaCl) with optima at pH 10.0, 55°C, and 3% NaCl. It retained 65 and 48% activities at pH 12.0 and 100°C, respectively. Partially purified protease was highly stable (100%) within pH range 7.0-12.0 and salinities of 0-5% NaCl for 48 hr. Cu 2+ , Mn 2+ , Co 2+ , and Ca 2+ did not inhibit its activity. Its stability at extreme pHs, temperatures, and in the presence of surfactants and commercial detergents suggests its possible application in laundry detergents. Partially purified protease was immobilized and reused. This is the first report of alkali-thermotolerant, surfactant-detergent-stable partially purified extracellular protease from A. caviae.

The Effect of Different Oceanic Abiotic Factors on Prokaryotic Body Sizes

NASA Astrophysics Data System (ADS)

Pidathala, S.; Bellon, M.; Heim, N.; Payne, J.

2016-12-01

We are studying the impact of abiotic factors in the Pacific and Atlantic on prokaryotic body sizes and genome sizes because we are interested in the manner in which abiotic factors influence genome sizes independent of their influence on body sizes. Some research has been done in the past on marine bacterial evolution, including data collection on marine ecology in relation to bacterial body sizes (Straza 2009). We are using the abiotic factors: temperature, salinity, and pH to compare the biovolumes/genome sizes of different phyla by using R. We made 9 scatter plots to model these relationships. Regardless of the phyla or the ocean, we found that there is no relation between pH, temperature, and body size, with several exceptions: Deinococcus. thermus has an indirect relationship with size in respect to temperature; size only correlates to temperature for phyla that are thermophiles. We also found that bacteria like D. thermus and Thermotogae are taxa only found in higher temperatures. Additionally, almost all phyla have genome sizes restricted by certain pH levels:, Proteobacteria only reach genomes with acidity levels greater than 6. In terms of salinity levels, certain bacteria are only found within a small range, and others, like Proteobacteria, can only reach genomes at low salinity levels. Finally, Proteobacteria have large genome sizes between 30 and 40 °, and Crenarchaeota have constant genome sizes in higher temperatures. Conclusively, we discovered that these abiotic factors generally do not affect body size, with the exception of D. thermus' indirect relationship to temperature due to its small biovolume in high temperatures. However, we determined that these abiotic factors have a great impact on genome sizes. This is due to genome size independence from body size. Also, genome size could have served as an adaptive feature for bacteria in marine environments, explaining why different phyla may have diverged to accommodate their lifestyles.

UV/TiO₂ photocatalytic oxidation of recalcitrant organic matter: effect of salinity and pH.

PubMed

Muthukumaran, Shobha; Song, Lili; Zhu, Bo; Myat, Darli; Chen, Jin-Yuan; Gray, Stephen; Duke, Mikel

2014-01-01

Photocatalytic oxidation processes have interest for water treatment since these processes can remove recalcitrant organic compounds and operate at mild conditions of temperature and pressure. However, performance under saline conditions present in many water resources is not well known. This study aims to explore the basic effects of photocatalysis on the removal of organic matter in the presence of salt. A laboratory-scale photocatalytic reactor system, employing ultraviolet (UV)/titanium dioxide (TiO₂) photocatalysis was evaluated for its ability to remove the humic acid (HA) from saline water. The particle size and zeta potential of TiO₂ under different conditions including solution pH and sodium chloride (NaCl) concentrations were characterized. The overall degradation of organics over the NaCl concentration range of 500-2,000 mg/L was found to be 80% of the non-saline equivalent after 180 min of the treatment. The results demonstrated that the adsorption of HA onto the TiO₂ particles was dependent on both the pH and salinity due to electrostatic interaction and highly unstable agglomerated dispersion. This result supports UV/TiO₂ as a viable means to remove organic compounds, but the presence of salt in waters to be treated will influence the performance of the photocatalytic oxidation process.

Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity

DOEpatents

Premuzic, Eugene T.; Lin, Mow

1996-02-20

This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70.degree. C. to 90.degree. C., at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%.

Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity

DOEpatents

Premuzic, E.T.; Lin, M.

1996-02-20

This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70 C to 90 C, at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%. 68 figs.

Study of Groundwater Physical Characteristics: A Case Study at District of Pekan, Pahang

NASA Astrophysics Data System (ADS)

Hashim, M. M. M.; Zawawi, M. H.; Samuding, K.; Dominic, J. A.; Zulkurnain, M. H.; Mohamad, K.

2018-04-01

A study of groundwater physical characteristic has been conducted at Pahang Tua, Pekan, Tanjung Batu and Nenasi, Pahang. There are several locations of tube well selected in this study. Four of five locations are situated in the coastal area and another one is located outside of coastal line. The purposes of this study are to identify the physical characteristic of groundwater (temperature, pH, electrical conductivity (EC), total dissolved solids (TDS) and salinity) and to identify the influence of sampling location and tube well depth to its physical characteristics. The results from the in-situ measurement were identified the physical characteristic groundwater for each tube well location. The result shows that temperature and pH for all groundwater samples almost in the same value but for the electrical conductivity, salinity and total dissolved solid have significant difference that related to location and depth of the tube well. The Pekan tube well with 80m depth and 2km distance from the sea have the highest value of EC, TDS and salinity (14460.53µS/cm, 7230.63 ppm and 8.32 PSU) compared to Nenasi with 30m depth of tube well and 0.65km distance from the sea. The EC, TDS and salinity value recorded are 1454.3253µS/cm, 727.00 ppm and 0.72 PSU. From the result of EC, TDS and salinity, it shows that the deeper tube well in the coastal area will obtained higher value of EC, TDS and salinity.

The Geographic Information System applied to study schistosomiasis in Pernambuco

PubMed Central

Barbosa, Verônica Santos; Loyo, Rodrigo Moraes; Guimarães, Ricardo José de Paula Souza e; Barbosa, Constança Simões

2017-01-01

ABSTRACT OBJECTIVE Diagnose risk environments for schistosomiasis in coastal localities of Pernambuco using geoprocessing techniques. METHODS A coproscopic and malacological survey were carried out in the Forte Orange and Serrambi areas. Environmental variables (temperature, salinity, pH, total dissolved solids and water fecal coliform dosage) were collected from Biomphalaria breeding sites or foci. The spatial analysis was performed using ArcGis 10.1 software, applying the kernel estimator, elevation map, and distance map. RESULTS In Forte Orange, 4.3% of the population had S. mansoni and were found two B. glabrata and 26 B. straminea breeding sites. The breeding sites had temperatures of 25ºC to 41ºC, pH of 6.9 to 11.1, total dissolved solids between 148 and 661, and salinity of 1,000 d. In Serrambi, 4.4% of the population had S. mansoni and were found seven B. straminea and seven B. glabrata breeding sites. Breeding sites had temperatures of 24ºC to 36ºC, pH of 7.1 to 9.8, total dissolved solids between 116 and 855, and salinity of 1,000 d. The kernel estimator shows the clusters of positive patients and foci of Biomphalaria, and the digital elevation map indicates areas of rainwater concentration. The distance map shows the proximity of the snail foci with schools and health facilities. CONCLUSIONS Geoprocessing techniques prove to be a competent tool for locating and scaling the risk areas for schistosomiasis, and can subsidize the health services control actions. PMID:29166439

Regional acidification trends in Florida shellfish estuaries: A 20+ year look at pH, oxygen, temperature, and salinity

USGS Publications Warehouse

Robbins, Lisa L.; Lisle, John T.

2018-01-01

Increasing global CO2 and local land use changes coupled with increased nutrient pollution are threatening estuaries worldwide. Local changes of estuarine chemistry have been documented, but regional associations and trends comparing multiple estuaries latitudinally have not been evaluated. Rapid climate change has impacted the annual and decadal chemical trends in estuaries, with local ecosystem processes enhancing or mitigating the responses. Here, we compare pH, dissolved oxygen, temperature, and salinity data from 10 Florida shellfish estuaries and hundreds of shellfish bed stations. Over 80,000 measurements, spanning from 1980 to 2008, taken on Atlantic Ocean and West Florida coast showed significant regional trends of consistent pH decreases in 8 out of the 10 estuaries, with an average rate of decrease on the Gulf of Mexico side estuaries of Florida of 7.3 × 10−4 pH units year−1, and average decrease on the Atlantic Coast estuaries of 5.0 × 10−4 pH units year−1. The rates are approximately 2–3.4 times slower than observed in pH decreases associated with ocean acidification in the Atlantic and Pacific. Other significant trends observed include decreasing dissolved oxygen in 9 out of the 10 estuaries, increasing salinity in 6 out of the 10, and temperature increases in 3 out of the 10 estuaries. The data provide a synoptic regional view of Florida estuary trends which reflect the complexity of changing climate and coastal ocean acidification superimposed on local conditions. These data provide context for understanding, and interpreting the past and predicting future of regional water quality health of shellfish and other organisms of commercial and ecological significance along Florida’s coasts.

Basic mechanism of button battery ingestion injuries and novel mitigation strategies after diagnosis and removal.

PubMed

Jatana, Kris R; Rhoades, Keith; Milkovich, Scott; Jacobs, Ian N

2017-06-01

Button battery (BB) injuries continue to be a significant source of morbidity and mortality, and there is a need to confirm the mechanism of injury for development of additional mitigation strategies. Cadaveric piglet esophageal model. Lithium, silver oxide, alkaline, and zinc-air BBs were placed in thawed sections of cadaveric piglet esophagus, bathed in normal saline. Severity of gross visual burn, pH, and temperature were recorded every 30 minutes for 6 hours. In other esophageal tissue specimens, the lithium BB was removed after 24, 36, and 48 hours and the site was irrigated with either 0.25% or 3% acetic acid. Separately, ReaLemon® juice, orange juice, Coke®, Dasani® water, Pepsi®, and saline were infused over a vertically suspended esophagus with a CR2032 lithium battery every 5 minutes for 2 hours while tissue temperature and pH were measured. A gradual rise in tissue pH and minimal change in temperature was noted for all BBs. ReaLemon® and orange juice applied every 5 minutes were most effective at neutralization of tissue pH with minimal change in tissue temperature. After BB removal (24, 36, 48 hours), irrigation of esophageal tissue specimens with 50-150 mL 0.25% acetic acid neutralized the highly alkaline tissue pH. BB appear to cause an isothermic hydrolysis reaction resulting in an alkaline caustic injury. Potential new mitigation strategies include application of neutralizing weakly acidic solutions that may reduce esophageal injury progression. NA Laryngoscope, 127:1276-1282, 2017. © 2016 The American Laryngological, Rhinological and Otological Society, Inc.

Effect of environmental factors on the germination and emergence of Salvia verbenaca L. cultivars (verbenaca and vernalis): An invasive species in semi-arid and arid rangeland regions

PubMed Central

Javaid, Muhammad Mansoor; Ali, Hafiz Haider; Weller, Sandra

2018-01-01

Salvia verbenaca (wild sage) is a commonly cultivated herbal medicine plant, which is native to the Mediterranean climate regions of Europe, Africa, Asia and the Middle East. However, it has become an invasive species in semi-arid and arid regions of southern Australia. Two varieties are present in this region, var. verbenaca and var. vernalis, each of which can be distinguished by differences in morphology and flowering period. Following trials to determine the optimum temperate regime for germination and response to light and dark, seeds of both varieties were tested for their response to variations in pH, moisture stress, salinity, and burial depth. The temperature and light trial was carried out using three different temperature regimes; 30/20°C, 25/15°C and 20/12°C, and two light regimes; 12 hours light/12 hours dark and 24 hours dark, with var. vernalis responding to relatively higher temperatures than var. verbenaca. The germination rate of neither species was significantly inhibited by complete darkness when compared to rates under periodic light exposure. Both varieties germinated at near optimum rates strongly to very strongly in all pH buffer solutions, from pH 5 to pH 10, but they responded most strongly at neutral pH. Var. vernalis showed slightly more tolerance to reduced moisture availability, moderate to strong salinity, and burial depth, compared to var. verbenaca. However, even a fairly shallow burial depth of 2 cm completely inhibited germination of both varieties. Thus, in circumstances where both varieties are present in a soil seedbank, var. vernalis could be expected to establish in more challenging conditions, where moisture is limited and salinity is ‘moderate to high’, implying that it is a more serious threat for invasive weed in conditions where crop plants are already challenged. PMID:29566039

Distribution of Surface pH and Total Alkalinity at the Sea of Okhotsk and the East Sea in October 2007

NASA Astrophysics Data System (ADS)

Shim, J.; Kang, D.; Jin, Y.; Obzhirov, A.

2008-12-01

Surface pH, total alkalinity, temperature and salinity were measured at the Sea of Okhotsk and the East Sea (along a track from Vladivostok to the northeastern slope of Sakhalin Island through Soya Strait: 42°N, 132°E - 55°N, 145°E) in October 2007. Continuous pH measurements were conducted using an underway potentiometric pH system modified from Tishchenko et al. (2002) and discrete total alkalinity measurements were made by direct titration with hydrochloric acid. Warm saline surface waters were observed in the East Sea (from Vladivostok to Soya Strait), and relatively cold less-saline waters were observed in the Sea of Okhotsk (at the eastern slopes of Sakhalin Island). In the East Sea and the Sea of Okhotsk, surface pH ranged from 8.063 to 8.158 and 8.047 to 8.226, and total alkalinity normalized to salinity 35 ranged from 2323 to 2344 μmol kg-1 and 2367 to 2422 μmol kg-1, respectively. Due to the freshwater input from rivers and geochemical activity in the water column and sediment, the Sea of Okhotsk generally showed much wider ranges of water properties and richer in carbonate parameters than those of the East Sea. Particularly, water properties changed dramatically at the eastern slopes of Sakhalin Island; surface salinity decreased southward by about 0.5-1 psu and pH and normalized total alkalinity increased southward by about 0.05-0.1 and 20-50 μmol kg-1, respectively. Thus, pCO2 concentration calculated from pH and total alkalinity, ranged from 350-375 μatm in the north to 280-300 μatm in the south of the Okhotsk Sea. The high pH and normalized total alkalinity, and low pCO2 and salinity in the south might be the result of surface water mixing with fresh water discharge from rivers and/or the results of massive primary production along the eastern coast of Sakhalin Island. In the most study area, surface pCO2 ranged from 280 to 370 μatm and was undersaturated relative to atmosphere. Therefore, the Sea of Okhotsk and the East Sea acted as effective CO2 sinks during the study period

The study of ikaite formation in sea ice

NASA Astrophysics Data System (ADS)

Hu, Y.; Nehrke, G.; Dieckmann, G.; Völker, C.; Wolf-Gladrow, D.

2012-04-01

Ikaite (CaCO3.6H2O) is a metastable mineral of calcium carbonate, which is usually found in environments characterized by low temperature (below 5° C), high pH, high alkalinity, high concentration of phosphate and organic matter. Although synthetic CaCO3.6H2O was already known from laboratory studies in 1865, ikaite was first observed in nature in 1963. Recently, Dieckmann et al. (2008, 2010) discovered this mineral in sea ice, which at the same time, was the first direct proof of CaCO3 precipitation in sea ice. However, little is known about the mechanism of ikaite formation in sea ice. Our study focuses on how physico-chemical processes in sea ice affect the formation of ikaite. Experiments were set up at pH ranging from 8.5 to 9.0, and salinity ranging from 0 to 105 at 0 ° C, in order to examine the effect of pH, salinity and also phosphate on the formation of ikaite. Preliminary results read: (1) Experiments show that ikaite can form at different pH levels (8.5~9.0). At high pH, the induction time (the time when the crystals start to precipitate) is shorter which means high pH favours the formation of ikaite. This might be expected given higher CO32- concentrations and thus higher saturation levels for ikaite with increasing pH. (2) The results of experiments with different salinities show that ikaite can form over wide range of salinities from 0 to 105 both in Artificial Sea Water (ASW) and NaCl solution in the presence of phosphate. In ASW, the induction time increases with salinity from S = 0 to S =105; while in NaCl solution, the induction time first increases with salinity and then decreases with the further increase of salinity. Salinity plays both positive and negative roles in the formation of ikaite. On the one hand, the increase in salinity will increase the fraction of CO32- in DIC. On the other hand, the increase in salinity means more ions are involved in the solution, which will reduce the activities of Ca2+ and CO32-by forming ion pairs with them. This effect is more obvious in ASW, as there are more ion species in ASW than in the NaCl solution. (3) The effect of different phosphate concentrations at high salinity (S = 70) medium show that in ASW, the precipitate is ikaite both with and without the presence of phosphate. In NaCl solution, the precipitate is ikaite in the presence of phosphate; however, the precipitate is no longer ikaite but vaterite in the absence of phosphate. These results suggest phosphate plays an important role in the formation of ikaite. However, besides phosphate, there must be other ion(s) in ASW, which also favour the formation of ikaite.

Combined effects of seawater acidification and salinity changes in Ruditapes philippinarum.

PubMed

Velez, Catia; Figueira, Etelvina; Soares, Amadeu M V M; Freitas, Rosa

2016-07-01

Due to human activities, predictions for the coming years indicate increasing frequency and intensity of extreme weather events (rainy and drought periods) and pollution levels, leading to salinity shifts and ocean acidification. Therefore, several authors have assessed the effects of seawater salinity shifts and pH decrease on marine bivalves, but most of these studies evaluated the impacts of both factors independently. Since pH and salinity may act together in the environment, and their impacts may differ from their effects when acting alone, there is an urgent need to increase our knowledge when these environmental changes act in combination. Thus, the present study assessed the effects of seawater acidification and salinity changes, both acting alone and in combination, on the physiological (condition index, Na and K concentrations) and biochemical (oxidative stress related biomarkers) performance of Ruditapes philippinarum. For that, specimens of R. philippinarum were exposed for 28days to the combination of different pH levels (7.8 and 7.3) and salinities (14, 28 and 35). The results obtained showed that under control pH (7.8) and low salinity (14) the physiological status and biochemical performance of clams was negatively affected, revealing oxidative stress. However, under the same pH and at salinities 28 and 35 clams were able to maintain/regulate their physiological status and biochemical performance. Moreover, our findings showed that clams under low pH (7.3) and different salinities were able to maintain their physiological status and biochemical performance, suggesting that the low pH tested may mask the negative effects of salinity. Our results further demonstrated that, in general, at each salinity, similar physiological and biochemical responses were found in clams under both tested pH levels. Also, individuals under low pH (salinities 14, 28 and 25) and exposed to pH 7.8 and salinity 28 (control) tend to present a similar response pattern. These results indicate that pH may have a lower impact on clams than salinity. Thus, our findings point out that the predicted increase of CO2 in seawater and consequent seawater acidification will have fewer impacts on physiological and biochemical performance of R. philippinarum clams than salinity shifts. Copyright © 2016 Elsevier B.V. All rights reserved.

The Effects of Elevated pCO2, Hypoxia and Temperature on Larval Sheepshead minnow, Cyprinodon variegatus: How much stress is too much?

EPA Science Inventory

Estuarine fish are acclimated to living in an environment with rapid and frequent changes in temperature, salinity, pH, and dissolved oxygen (DO) levels; the physiology of these organisms is well suited to cope with extreme thermal, hypercapnic, and hypoxic stress. While the adve...

Water and sediment characteristics associated with avian botulism outbreaks in wetlands

USGS Publications Warehouse

Rocke, Tonie E.; Samuel, Michael D.

1999-01-01

Avian botulism kills thousands of waterbirds annually throughout North America, but management efforts to reduce its effects have been hindered because environmental conditions that promote outbreaks are poorly understood. We measured sediment and water variables in 32 pairs of wetlands with and without a current outbreak of avian botulism. Wetlands with botulism outbreaks had greater percent organic matter (POM) in the sediment (P = 0.088) and lower redox potential in the water (P = 0.096) than paired control wetlands. We also found that pH, redox potential, temperature, and salinity measured just above the sediment-water interface were associated (P ≤ 0.05) with the risk of botulism outbreaks in wetlands, but relations were complex, involving nonlinear and multivariate associations. Regression models indicated that the risk of botulism outbreaks increased when water pH was between 7.5 and 9.0, redox potential was negative, and water temperature was >20°C. Risk declined when redox potential increased (>100), water temperature decreased (10-15°C), pH was 9.0, or salinity was low (<2.0 ppt). Our predictive models could allow managers to assess potential effects of wetland management practices on the risk of botulism outbreaks and to develop and evaluate alternative management strategies to reduce losses from avian botulism.

Effects of Nutrients, Salinity, and pH on Salvinia molesta (Mitchell) Growth

DTIC Science & Technology

2010-07-01

Smart PURPOSE: This study documents effects of macronutrient (nitrogen and phosphorus) concentra- tions, pH, and salinity on giant salvinia growth...investigated. The objective of this study was to document effects of macronutrient (nitrogen and phosphorus) concentrations, pH, and salinity on giant...giant salvinia to become problematic, including low pH, low salinity, and the presence of suitable macronutrients . In combination with distribution

U.S. Coast Guard 1994 Oil Pollution Research Grants Publications - Part II.

DTIC Science & Technology

1996-09-01

preparations. However, toxicity testing in various salinities may be of future interest in a number of cases. We have no direct data on the effect of different...of oil toxicity. Factors such as temperature (Korn et al. 1979), salinity (Linden et al. 1979), pH and route of exposure (Lee et al. 1976) may effect ...Evidence of altered metabolic pathways provides information concerning enzyme systems sensitive to oil-dispersant expostre. Any factors effecting these

Water quality in Rhode River at Smithsonian Institution Pier near Annapolis, Maryland, January 1976 through December 1978

USGS Publications Warehouse

Cory, Robert L.; Dresler, P.V.

1980-01-01

Water temperature, salinity, turbidity, dissolved oxygen, pH, and water level data were continuously monitored and recorded from the Smithsonian Institution pier near Annapolis, Md., from January 1976 through December 1978. Daily maximum and minimum values are tabulated and summarized, and monthly averages and extremes are presented. Water temperature ranged from 0.0 to 33.9 Celsius. Both high and low extreme values exceeded those recorded during the previous 6 years. Salinity patterns showed normal seasonal variations and were related to the Susquehanna River inflow, which controls the upper bay salinity. Salinity between 13 and 15 parts per thousand in November and December 1978 were the highest recorded over a 9-year period. Turbidity varied seasonally, with lowest values in winter and highest in spring. Dissolved oxygen ranged from 2.0 to 18.7 milligrams per liter. Large variations between summertime daily minima and maxima indicated the high state of eutrophication of the water being monitored. Hydrogen-ion activity (pH) ranged from 7.0 to 10.2 over the 3-year period. The pH changes reflect daily variation in partial pressure of carbon dioxide, which varies inversely with the dissolved oxygen. Water level variation at the monitoring site for the 3-year period was 1.89 meters, with highest water 0.59 meter above mean high water and lowest 0.83 meter below mean low water. An apparent decline of 0.07 meter below previously recorded mean high and mean low water was associated with stronger winds and a prevalance of westerly winds in February during the winter of 1976-1977. (USGS)

Short-Term Exposure of Mytilus coruscus to Decreased pH and Salinity Change Impacts Immune Parameters of Their Haemocytes.

PubMed

Wu, Fangli; Xie, Zhe; Lan, Yawen; Dupont, Sam; Sun, Meng; Cui, Shuaikang; Huang, Xizhi; Huang, Wei; Liu, Liping; Hu, Menghong; Lu, Weiqun; Wang, Youji

2018-01-01

With the release of large amounts of CO 2 , ocean acidification is intensifying and affecting aquatic organisms. In addition, salinity also plays an important role for marine organisms and fluctuates greatly in estuarine and coastal ecosystem, where ocean acidification frequently occurs. In present study, flow cytometry was used to investigate immune parameters of haemocytes in the thick shell mussel Mytilus coruscus exposed to different salinities (15, 25, and 35‰) and two pH levels (7.3 and 8.1). A 7-day in vivo and a 5-h in vitro experiments were performed. In both experiments, low pH had significant effects on all tested immune parameters. When exposed to decreased pH, total haemocyte count (THC), phagocytosis (Pha), esterase (Est), and lysosomal content (Lyso) were significantly decreased, whereas haemocyte mortality (HM) and reactive oxygen species (ROS) were increased. High salinity had no significant effects on the immune parameters of haemocytes as compared with low salinity. However, an interaction between pH and salinity was observed in both experiments for most tested haemocyte parameters. This study showed that high salinity, low salinity and low pH have negative and interactive effects on haemocytes of mussels. As a consequence, it can be expected that the combined effect of low pH and changed salinity will have more severe effects on mussel health than predicted by single exposure.

Physiological tolerances of juvenile robust redhorse, Moxostoma robustum: Conservation implications for an imperiled species

USGS Publications Warehouse

Walsh, S.J.; Haney, D.C.; Timmerman, C.M.; Dorazio, R.M.

1998-01-01

The robust redhorse, Moxostoma robustum (Teleostei: Catostomidae), is an imperiled sucker native to large rivers of the Atlantic slope of the southeastern United States. Juvenile M. robustum were tested for tolerances to temperature, salinity, pH, and hypoxia in order to evaluate basic early life-history requirements. Static (acute) tests resulted in estimates of mean lower temperature tolerances (5.3-19.4 ??C) that varied with prior thermal acclimation and indicated no apparent difference in tolerance among fish 30, 60, and 90 days old. Fish acclimated to 20 ??C and 30 ??C had significantly different mean critical thermal maxima (34.9 ??C and 37.2 ??C, respectively) and exhibited pronounced increased opercular ventilation rates with elevated temperatures. Fish exposed to acute and chronic increases in salinity showed unusual patterns of mortality above the isosmotic point (9 ppt) that reflected possible differences in body mass and prior acclimation conditions (i.e., water ionic composition); small fish and those held in soft water were the least tolerant of increased salinity. Abrupt exposure to extreme pH values resulted in greater than 50% mortality at pH values below 4.3 and above 9.5 within a 96-hour period. Fish exposed to progressive hypoxia utilized aquatic surface respiration at a mean oxygen concentration of 0.72-0.80 mg O2 l-1 (20 ??C and 30 ??C acclimated fish, respectively), and lost equilibrium at 0.54-.57 mg O2 l-1. Juvenile M. robustum are moderately tolerant of a wide range of ambient physicochemical parameters, but further research is needed to determine how both abiotic and biotic factors have contributed to population decline and extirpation of this species.

[Adaptability of abnormal tadpole (Rana chensinensis) to water pH, salinity and alkalinity in Changbai Mountain of China].

PubMed

Yang, Fuyi; Shao, Qingchun; Li, Jinglin; Chen, Guoshuang

2004-08-01

Under field condition with 16-18 degree C water temperature, single-factor acute toxicity test was used to study the toxicity effects of water pH, salinity and carbonate-alkalinity on abnormal tadpole (R. chensinensis). The results showed that when the water salinity was 0.18 g x L(-1), carbonate-alkalinity was 1.41 mmol x L(-1), and water pH was 4.3-9.7, the survival rate of abnormal tadpole within 96 hours was not affected. The upper limit of LC50 for the pH within 24, 48, 72 and 96 hours was 10.33, 10.18, 10.08 and 10.02, and the prescribed minimum was 3.92, 4.07, 4.11 and 4.16, respectively. The upper limit of LC0 was 9.95, 9.80, 9.70 and 9.70, and the prescribed minimum was 4.23, 4.45, 4.30 and 4.30, and that of LC100 was 10.70, 10.55, 10.45 and 10.33, and the prescribed minimum was 3.55, 3.70, 3.92 and 4.03, respectively. The survival rate of abnormal tadpole within 96 hours was not affected in the water salinity between 2.0-3.0 g x L(-1). When water pH was 7.0-8.5 and carbonate-alkalinity was 1.41 mmol x L(-1), the LC50 of the salinity within 24, 48, 72 and 96 hours was 8.21, 7.25, 5.17 and 3.70 g x L(-1), the LC0 was 7.14, 6.00, 2.67 and 2.20 g x L(-1), and the LC100 was 9.98, 9.00, 7.67 and 5.20 g x L(-1), respectively, while the SC was 1.70 g x L(-1). Under the same water pH and when the water salinity was 0.18 g x L(-1), the LC50 of carbonate-alkalinity within 24, 48, 72 and 96 hours was 14.36, 11.83, 10.35, and 7.68 mmol x L(-1), the LC0 was 8.76, 8.51, 4.65 and 3.88 mmol x L(-1), and the LC100 was 19.96, 15.14, 16.05 and 11.48 mmol x L(-1), respectively, while the SC was 1.70 mmol x L(-1). The survival rate of abnormal tadpole (R. chensinensis) was decreased with increasing water pH, salinity and carbonate-alkalinity. The optimum water salinity and carbonate-alkalinity to the survival and the growth of abnormal tadpole (R. chensinensis) were below 2.0 g x L(-1) and 3.0 mmol x L(-1), respectively, and water pH was between 6.0 and 9.0.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Renaut, R.W.; Owen, R.B.

An unusual group of cherts found at saline, alkaline Lake Bogoria in the Kenya Rift differs from the Magadi-type cherts commonly associated with saline, alkaline lakes. The cherts are opaline, rich in diatoms, and formed from a siliceous, probably gelatinous, precursor that precipitated around submerged alkaline hot springs during a Holocene phase of high lake level. Silica precipitation resulted from rapid drop in the temperature of the spring waters and, possibly, pH. Lithification began before subaerial exposure. Ancient analogous cherts are likely to be localized deposits along fault lines.

Effects of culture conditions on growth and docosahexaenoic acid production from Schizochytrium limacinum

NASA Astrophysics Data System (ADS)

Zhu, Luying; Zhang, Xuecheng; Ren, Xueying; Zhu, Qinghua

2008-02-01

The effects of temperature, initial pH, salinity of culture medium, and carbon and nitrogen sources on growth and docosahexaenoic acid (C22: 6 n-3, DHA) production from Schizochytrium limacinum OUC88 were investigated in the present study. The results revealed that the optimal temperature, initial pH and salinity level of the medium for DHA production were 23°C, 7.0 and 18, respectively. Glucose was proved the best carbon source for the growth and DHA production from S. limacinum. Among the nitrogen sources tested, soybean cake hydrolysate, a cheap by-product, was found to be effective for the accumulation of DHA in S. limacinum cells. In addition, increasing the concentration of carbon sources in the medium caused a significant increase in cell biomass; however, accumulation of DHA in cells was mainly stimulated by the ratio of C/N in the medium. Under the optimal culture conditions, the maximum DHA yield achieved in flasks was 4.08 g L-1 after 5 d of cultivation.

Characterisation and deployment of an immobilised pH sensor spot towards surface ocean pH measurements.

PubMed

Clarke, Jennifer S; Achterberg, Eric P; Rérolle, Victoire M C; Abi Kaed Bey, Samer; Floquet, Cedric F A; Mowlem, Matthew C

2015-10-15

The oceans are a major sink for anthropogenic atmospheric carbon dioxide, and the uptake causes changes to the marine carbonate system and has wide ranging effects on flora and fauna. It is crucial to develop analytical systems that allow us to follow the increase in oceanic pCO2 and corresponding reduction in pH. Miniaturised sensor systems using immobilised fluorescence indicator spots are attractive for this purpose because of their simple design and low power requirements. The technology is increasingly used for oceanic dissolved oxygen measurements. We present a detailed method on the use of immobilised fluorescence indicator spots to determine pH in ocean waters across the pH range 7.6-8.2. We characterised temperature (-0.046 pH/°C from 5 to 25 °C) and salinity dependences (-0.01 pH/psu over 5-35), and performed a preliminary investigation into the influence of chlorophyll on the pH measurement. The apparent pKa of the sensor spots was 6.93 at 20 °C. A drift of 0.00014 R (ca. 0.0004 pH, at 25 °C, salinity 35) was observed over a 3 day period in a laboratory based drift experiment. We achieved a precision of 0.0074 pH units, and observed a drift of 0.06 pH units during a test deployment of 5 week duration in the Southern Ocean as an underway surface ocean sensor, which was corrected for using certified reference materials. The temperature and salinity dependences were accounted for with the algorithm, R=0.00034-0.17·pH+0.15·S(2)+0.0067·T-0.0084·S·1.075. This study provides a first step towards a pH optode system suitable for autonomous deployment. The use of a short duration low power illumination (LED current 0.2 mA, 5 μs illumination time) improved the lifetime and precision of the spot. Further improvements to the pH indicator spot operations include regular application of certified reference materials for drift correction and cross-calibration against a spectrophotometric pH system. Desirable future developments should involve novel fluorescence spots with improved response time and apparent pKa values closer to the pH of surface ocean waters. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

Short-Term Exposure of Mytilus coruscus to Decreased pH and Salinity Change Impacts Immune Parameters of Their Haemocytes

PubMed Central

Wu, Fangli; Xie, Zhe; Lan, Yawen; Dupont, Sam; Sun, Meng; Cui, Shuaikang; Huang, Xizhi; Huang, Wei; Liu, Liping; Hu, Menghong; Lu, Weiqun; Wang, Youji

2018-01-01

With the release of large amounts of CO2, ocean acidification is intensifying and affecting aquatic organisms. In addition, salinity also plays an important role for marine organisms and fluctuates greatly in estuarine and coastal ecosystem, where ocean acidification frequently occurs. In present study, flow cytometry was used to investigate immune parameters of haemocytes in the thick shell mussel Mytilus coruscus exposed to different salinities (15, 25, and 35‰) and two pH levels (7.3 and 8.1). A 7-day in vivo and a 5-h in vitro experiments were performed. In both experiments, low pH had significant effects on all tested immune parameters. When exposed to decreased pH, total haemocyte count (THC), phagocytosis (Pha), esterase (Est), and lysosomal content (Lyso) were significantly decreased, whereas haemocyte mortality (HM) and reactive oxygen species (ROS) were increased. High salinity had no significant effects on the immune parameters of haemocytes as compared with low salinity. However, an interaction between pH and salinity was observed in both experiments for most tested haemocyte parameters. This study showed that high salinity, low salinity and low pH have negative and interactive effects on haemocytes of mussels. As a consequence, it can be expected that the combined effect of low pH and changed salinity will have more severe effects on mussel health than predicted by single exposure. PMID:29559924

Six Month In Situ High-Resolution Carbonate Chemistry and Temperature Study on a Coral Reef Flat Reveals Asynchronous pH and Temperature Anomalies.

PubMed

Kline, David I; Teneva, Lida; Hauri, Claudine; Schneider, Kenneth; Miard, Thomas; Chai, Aaron; Marker, Malcolm; Dunbar, Rob; Caldeira, Ken; Lazar, Boaz; Rivlin, Tanya; Mitchell, Brian Gregory; Dove, Sophie; Hoegh-Guldberg, Ove

2015-01-01

Understanding the temporal dynamics of present thermal and pH exposure on coral reefs is crucial for elucidating reef response to future global change. Diel ranges in temperature and carbonate chemistry parameters coupled with seasonal changes in the mean conditions define periods during the year when a reef habitat is exposed to anomalous thermal and/or pH exposure. Anomalous conditions are defined as values that exceed an empirically estimated threshold for each variable. We present a 200-day time series from June through December 2010 of carbonate chemistry and environmental parameters measured on the Heron Island reef flat. These data reveal that aragonite saturation state, pH, and pCO2 were primarily modulated by biologically-driven changes in dissolved organic carbon (DIC) and total alkalinity (TA), rather than salinity and temperature. The largest diel temperature ranges occurred in austral spring, in October (1.5 - 6.6°C) and lowest diel ranges (0.9 - 3.2°C) were observed in July, at the peak of winter. We observed large diel total pH variability, with a maximum range of 7.7 - 8.5 total pH units, with minimum diel average pH values occurring during spring and maximum during fall. As with many other reefs, the nighttime pH minima on the reef flat were far lower than pH values predicted for the open ocean by 2100. DIC and TA both increased from June (end of Fall) to December (end of Spring). Using this high-resolution dataset, we developed exposure metrics of pH and temperature individually for intensity, duration, and severity of low pH and high temperature events, as well as a combined metric. Periods of anomalous temperature and pH exposure were asynchronous on the Heron Island reef flat, which underlines the importance of understanding the dynamics of co-occurrence of multiple stressors on coastal ecosystems.

Six Month In Situ High-Resolution Carbonate Chemistry and Temperature Study on a Coral Reef Flat Reveals Asynchronous pH and Temperature Anomalies

PubMed Central

Kline, David I.; Teneva, Lida; Hauri, Claudine; Schneider, Kenneth; Miard, Thomas; Chai, Aaron; Marker, Malcolm; Dunbar, Rob; Caldeira, Ken; Lazar, Boaz; Rivlin, Tanya; Mitchell, Brian Gregory; Dove, Sophie; Hoegh-Guldberg, Ove

2015-01-01

Understanding the temporal dynamics of present thermal and pH exposure on coral reefs is crucial for elucidating reef response to future global change. Diel ranges in temperature and carbonate chemistry parameters coupled with seasonal changes in the mean conditions define periods during the year when a reef habitat is exposed to anomalous thermal and/or pH exposure. Anomalous conditions are defined as values that exceed an empirically estimated threshold for each variable. We present a 200-day time series from June through December 2010 of carbonate chemistry and environmental parameters measured on the Heron Island reef flat. These data reveal that aragonite saturation state, pH, and pCO2 were primarily modulated by biologically-driven changes in dissolved organic carbon (DIC) and total alkalinity (TA), rather than salinity and temperature. The largest diel temperature ranges occurred in austral spring, in October (1.5 – 6.6°C) and lowest diel ranges (0.9 – 3.2°C) were observed in July, at the peak of winter. We observed large diel total pH variability, with a maximum range of 7.7 – 8.5 total pH units, with minimum diel average pH values occurring during spring and maximum during fall. As with many other reefs, the nighttime pH minima on the reef flat were far lower than pH values predicted for the open ocean by 2100. DIC and TA both increased from June (end of Fall) to December (end of Spring). Using this high-resolution dataset, we developed exposure metrics of pH and temperature individually for intensity, duration, and severity of low pH and high temperature events, as well as a combined metric. Periods of anomalous temperature and pH exposure were asynchronous on the Heron Island reef flat, which underlines the importance of understanding the dynamics of co-occurrence of multiple stressors on coastal ecosystems. PMID:26039687

Use of intertidal areas by shrimps (Decapoda) in a Brazilian Amazon estuary.

PubMed

Sampaio, Hebert A; Martinelli-Lemos, Jussara M

2014-03-01

The present work investigated the occupation and the correlation of the shrimp abundance in relation to environmental variables in different habitats (mangroves, salt marshes and rocky outcrops) in an Amazon estuary. The collections were made in August and November 2009, at low syzygy tide on Areuá Beach, situated in the Extractive Reserve of Mãe Grande de Curuçá, Pará, Brazil totaling 20 pools. In each environment, we recorded the physical-chemical factors (pH, salinity, and temperature) and measured the area (m2) and volume (m3) of every pool through bathymetry. The average pH, salinity, temperature, area and volume of tide pools were 8.75 (± 0.8 standard deviation), 35.45 (± 3), 29.49 °C (± 2.32), 27.41 m2 (± 41.18), and 5.19 m3 (± 8.01), respectively. We caught a total of 4,871 shrimps, distributed in three families and four species: Farfantepenaeus subtilis (98.36%) (marine) followed by Alpheus pontederiae (0.76%) (estuarine), Macrobrachium surinamicum (0.45%) and Macrobrachium amazonicum (0.43%) predominantly freshwater. The species F. subtilis and A. pontederiae occurred in the three habitats, whereas M. surinamicum occurred in salt marsh and rocky outcrop and M. amazonicum only in marisma. Temperature and pH were the most important environmental descriptors that significantly affected the density and biomass of shrimps.

Interactions between chemical and climate stressors: A role for mechanistic toxicology in assessing climate change risks

EPA Science Inventory

Incorporation of global climate change (GCC) effects into regulatory assessments of chemical risk and injury requires an integrated examination of both chemical and non-chemical stressors. Environmental variables altered by GCC, such as temperature, precipitation, salinity and pH...

Synergistic and Antagonistic Effects of Salinity and pH on Germination in Switchgrass (Panicum virgatum L.)

PubMed Central

Liu, Yuan; Wang, Quanzhen; Zhang, Yunwei; Cui, Jian; Chen, Guo; Xie, Bao; Wu, Chunhui; Liu, Haitao

2014-01-01

The effects of salt-alkaline mixed stress on switchgrass were investigated by evaluating seed germination and the proline, malondialdehyde (MDA) and soluble sugar contents in three switchgrass (Panicum virgatum L.) cultivars in order to identify which can be successfully produced on marginal lands affected by salt-alkaline mixed stress. The experimental conditions consisted of four levels of salinity (10, 60, 110 and 160 mM) and four pH levels (7.1, 8.3, 9.5 and 10.7). The effects of salt-alkaline mixed stress with equivalent coupling of the salinity and pH level on the switchgrass were explored via model analyses. Switchgrass was capable of germinating and surviving well in all treatments under low-alkaline pH (pH≤8.3), regardless of the salinity. However, seed germination and seedling growth were sharply reduced at higher pH values in conjunction with salinity. The salinity and pH had synergetic effects on the germination percentage, germination index, plumular length and the soluble sugar and proline contents in switchgrass. However, these two factors exhibited antagonistic effects on the radicular length of switchgrass. The combined effects of salinity and pH and the interactions between them should be considered when evaluating the strength of salt-alkaline mixed stress. PMID:24454834

Global Biogeographic Analysis of Methanogenic Archaea Identifies Community-Shaping Environmental Factors of Natural Environments

PubMed Central

Wen, Xi; Yang, Sizhong; Horn, Fabian; Winkel, Matthias; Wagner, Dirk; Liebner, Susanne

2017-01-01

Methanogenic archaea are important for the global greenhouse gas budget since they produce methane under anoxic conditions in numerous natural environments such as oceans, estuaries, soils, and lakes. Whether and how environmental change will propagate into methanogenic assemblages of natural environments remains largely unknown owing to a poor understanding of global distribution patterns and environmental drivers of this specific group of microorganisms. In this study, we performed a meta-analysis targeting the biogeographic patterns and environmental controls of methanogenic communities using 94 public mcrA gene datasets. We show a global pattern of methanogenic archaea that is more associated with habitat filtering than with geographical dispersal. We identify salinity as the control on methanogenic community composition at global scale whereas pH and temperature are the major controls in non-saline soils and lakes. The importance of salinity for structuring methanogenic community composition is also reflected in the biogeography of methanogenic lineages and the physiological properties of methanogenic isolates. Linking methanogenic alpha-diversity with reported values of methane emission identifies estuaries as the most diverse methanogenic habitats with, however, minor contribution to the global methane budget. With salinity, temperature and pH our study identifies environmental drivers of methanogenic community composition facing drastic changes in many natural environments at the moment. However, consequences of this for the production of methane remain elusive owing to a lack of studies that combine methane production rate with community analysis. PMID:28769904

Downhole Transformation of the Hydraulic Fracturing Fluid Biocide Glutaraldehyde: Implications for Flowback and Produced Water Quality.

PubMed

Kahrilas, Genevieve A; Blotevogel, Jens; Corrin, Edward R; Borch, Thomas

2016-10-18

Hydraulic fracturing fluid (HFF) additives are used to enhance oil and gas extraction from unconventional shale formations. Several kilometers downhole, these organic chemicals are exposed to temperatures up to 200 °C, pressures above 10 MPa, high salinities, and a pH range from 5-8. Despite this, very little is known about the fate of HFF additives under these extreme conditions. Here, stainless steel reactors are used to simulate the downhole chemistry of the commonly used HFF biocide glutaraldehyde (GA). The results show that GA rapidly (t 1/2 < 1 h) autopolymerizes, forming water-soluble dimers and trimers, and eventually precipitates out at high temperatures (∼140 °C) and/or alkaline pH. Interestingly, salinity was found to significantly inhibit GA transformation. Pressure and shale did not affect GA transformation and/or removal from the bulk fluid. On the basis of experimental pseudo-second-order rate constants, a kinetic model for GA downhole half-life predictions for any combination of these conditions within the limits tested was developed. These findings illustrate that the biocidal GA monomer has limited time to control microbial activity in hot and/or alkaline shales, and may return along with its aqueous transformation products to the surface via flowback and produced water in cooler, more acidic, and saline shales.

[Spatial differentiation and impact factors of Yutian Oasis's soil surface salt based on GWR model].

PubMed

Yuan, Yu Yun; Wahap, Halik; Guan, Jing Yun; Lu, Long Hui; Zhang, Qin Qin

2016-10-01

In this paper, topsoil salinity data gathered from 24 sampling sites in the Yutian Oasis were used, nine different kinds of environmental variables closely related to soil salinity were selec-ted as influencing factors, then, the spatial distribution characteristics of topsoil salinity and spatial heterogeneity of influencing factors were analyzed by combining the spatial autocorrelation with traditional regression analysis and geographically weighted regression model. Results showed that the topsoil salinity in Yutian Oasis was not of random distribution but had strong spatial dependence, and the spatial autocorrelation index for topsoil salinity was 0.479. Groundwater salinity, groundwater depth, elevation and temperature were the main factors influencing topsoil salt accumulation in arid land oases and they were spatially heterogeneous. The nine selected environmental variables except soil pH had significant influences on topsoil salinity with spatial disparity. GWR model was superior to the OLS model on interpretation and estimation of spatial non-stationary data, also had a remarkable advantage in visualization of modeling parameters.

Pilot-scale studies on biological treatment of hypersaline wastewater at low temperature.

PubMed

Peng, Y Z; Zhu, G B; Wang, S Y; Yu, D S; Cui, Y W; Meng, X S

2005-01-01

In order to investigate the feasibility of biological treatment of hypersaline wastewater produced from toilet flushing with seawater at low temperature, pilot-scale studies were established with plug-flow activated sludge process at low temperature (5-9 degrees C) based on bench-scale experiments. The critical salinity concentration of 30 g/L, which resulted from the cooperation results of the non-halophilic bacteria and the halophilic bacteria, was drawn in bench-scale experiments. Pilot-scale studies showed that high COD removal efficiency, higher than 80%, was obtained at low temperature when 30 percent seawater was introduced. The salinity improved the settleability of activated sludge, and average sludge value dropped down from 38% to 22.5% after adding seawater. Seawater salinity had a strong negative effect on notronomonas and nitrobacter growth, but much more on the nitrobacter. The nitrification action was mainly accomplished by nitrosomonas. Bench-scale experiments using two SBRs were carried out for further investigation under different conditions of salinities, ammonia loadings and temperatures. Biological nitrogen removal via nitrite pathway from wastewater containing 30 percent seawater was achieved, but the ammonia removal efficiency was strongly related not only to the influent ammonia loading at different salinities but also to temperature. When the ratio of seawater to wastewater was 30 percent, and the ammonia loading was below the critical value of 0.15 kgNH4+-N/(kgMLSS.d), the ammonia removal efficiency via nitrite pathway was above 90%. The critical level of ammonia loading was 0.15, 0.08 and 0.03 kgNH4+-N/(kgMLSS.d) respectively at the different temperature 30 degrees C, 25 degrees C and 20 degrees C when the influent ammonia concentration was 60-80 mg/L and pH was 7.5-8.0.

Short term exposure to elevated pCO2 and hypoxia affects the cellular homeostasis of grass shrimp, Palaemonetes pugio

EPA Science Inventory

Estuarine organisms are adapted to frequent changes in temperature, salinity, pH, and dissolved oxygen (DO) levels. The high productivity of an estuary contributes to large changes in environmental conditions, with organismal respiration enhancing hypoxic zones, and elevating pCO...

A Pathway-based Approach to Predicting Interactions between Chemical and Non-chemical Stressors: Applications to Global Climate Change

EPA Science Inventory

A variety of environmental variables influenced by global climate change (GCC) can directly or indirectly affect the health of organisms. These variables may include temperature, salinity, pH, and penetration of ultraviolet radiation (UVR) in aquatic environments, and water shor...

TRAIT-BASED FRAMEWORK TO PREDICTING RELATIVE VULNERABILITY TO CLIMATE CHANGE IN NEAR-COASTAL SPECIES AND HABITAT

EPA Science Inventory

Like Icarus, near-coastal species are “flying too close” to the sun, and are being impacted by climate-induced changes in air and ocean temperature, precipitation, salinity, ocean pH, sea level rise, and nonindigenous species. Sound management requires knowledge of wh...

Implementation of PLUTO Buoy for Monitoring Water Quality in Indonesia, Reflection and Future Plans

NASA Astrophysics Data System (ADS)

Chandra, H.; Krismono, K.; Kusumaningrum, P. D.; Sianturi, D.; Firdaus, Y.; Taukhid, I.; Borneo, B. B.

2016-02-01

Research and development of PLUTO (Perairan Selalu Termonitor/Waters Always Monitored) buoy has reached its fourth year in 2015. Try out has been done in coastal waters, fishponds, fishing port ponds, and reservoirs. In the first year (2010) try out has been performed on coastal waters with off line measurement system. The buoy used temperature, salinity, DO and pH sensors. In the second year (2013) try out was carried out on fishponds and fishing port ponds using telemetry measurement system. In the third year (2014) try out was carried out on water reservoir with telemetry measurement system. In the fourth year (2015) android application is developed to monitor 4 water reservoirs and 1 lake. Beside that, observation point is added to 3 point depth for one buoy. Parameters used are temperature, DO, and turbidity. Three PLUTO buoys are placed in each reservoir, at inlet, outlet, and at center of fish cultivation. Through Ocean Science Meeting in New Orleans it is hoped that there will be input and suggestion from the experts for future development of the monitoring system for public inland waters (especially reservoir and lake) in Indonesia. Keywords: buoy PLUTO, salinity, temperature, Dissolved Oxygen (DO), pH, turbidity, telemetry

Biology, ecology, and biotechnological applications of anaerobic bacteria adapted to environmental stresses in temperature, pH, salinity, or substrates.

PubMed Central

Lowe, S E; Jain, M K; Zeikus, J G

1993-01-01

Anaerobic bacteria include diverse species that can grow at environmental extremes of temperature, pH, salinity, substrate toxicity, or available free energy. The first evolved archaebacterial and eubacterial species appear to have been anaerobes adapted to high temperatures. Thermoanaerobes and their stable enzymes have served as model systems for basic and applied studies of microbial cellulose and starch degradation, methanogenesis, ethanologenesis, acetogenesis, autotrophic CO2 fixation, saccharidases, hydrogenases, and alcohol dehydrogenases. Anaerobes, unlike aerobes, appear to have evolved more energy-conserving mechanisms for physiological adaptation to environmental stresses such as novel enzyme activities and stabilities and novel membrane lipid compositions and functions. Anaerobic syntrophs do not have similar aerobic bacterial counterparts. The metabolic end products of syntrophs are potent thermodynamic inhibitors of energy conservation mechanisms, and they require coordinated consumption by a second partner organism for species growth. Anaerobes adapted to environmental stresses and their enzymes have biotechnological applications in organic waste treatment systems and chemical and fuel production systems based on biomass-derived substrates or syngas. These kinds of anaerobes have only recently been examined by biologists, and considerably more study is required before they are fully appreciated by science and technology. Images PMID:8336675

Equilibrium partitioning of organic compounds to OASIS HLB® as a function of compound concentration, pH, temperature and salinity.

PubMed

Jeong, Yoonah; Schäffer, Andreas; Smith, Kilian

2017-05-01

Oasis hydrophilic lipophilic balance ® (Oasis HLB) is commonly employed in solid phase extraction (SPE) of environmental contaminants and within polar organic chemical integrative passive samplers (POCIS). In this study batch experiments were carried out to evaluate the relative affinity of a range of relevant organic pollutants to Oasis HLB in aqueous systems. The influence of sorbate concentration, temperature, pH, and salinity on the equilibrium sorption was investigated. Equilibrium partition ratios (K D ) of 28 compounds were determined, ranging over three orders of magnitude from 1.16 × 10 3  L/kg (atenolol) to 1.07 × 10 6  L/kg (isoproturon). The Freundlich model was able to describe the equilibrium partitioning to Oasis HLB, and an analysis of the thermodynamic parameters revealed the spontaneous and exothermic nature of the partitioning process. Ionic strength had only a minor effect on the partitioning, whereas pH had a considerable effect but only for ionizable compounds. The results show that apolar interactions between the Oasis HLB and analyte mainly determine the equilibrium partitioning. These research findings can be used to optimize the application of SPE and POCIS for analyses of environmental contaminants even in complex mixtures. Copyright © 2017 Elsevier Ltd. All rights reserved.

A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum.

PubMed

Sordo, Laura; Santos, Rui; Reis, Joao; Shulika, Alona; Silva, Joao

2016-01-01

Most ocean acidification (OA) experimental systems rely on pH as an indirect way to control CO 2 . However, accurate pH measurements are difficult to obtain and shifts in temperature and/or salinity alter the relationship between pH and p CO 2 . Here we describe a system in which the target p CO 2 is controlled via direct analysis of p CO 2 in seawater. This direct type of control accommodates potential temperature and salinity shifts, as the target variable is directly measured instead of being estimated. Water in a header tank is permanently re-circulated through an air-water equilibrator. The equilibrated air is then routed to an infrared gas analyzer (IRGA) that measures p CO 2 and conveys this value to a Proportional-Integral-Derivative (PID) controller. The controller commands a solenoid valve that opens and closes the CO 2 flush that is bubbled into the header tank. This low-cost control system allows the maintenance of stabilized levels of p CO 2 for extended periods of time ensuring accurate experimental conditions. This system was used to study the long term effect of OA on the coralline red algae Phymatolithon lusitanicum . We found that after 11 months of high CO 2 exposure, photosynthesis increased with CO 2 as opposed to respiration, which was positively affected by temperature. Results showed that this system is adequate to run long-term OA experiments and can be easily adapted to test other relevant variables simultaneously with CO 2 , such as temperature, irradiance and nutrients.

A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum

PubMed Central

Santos, Rui; Reis, Joao; Shulika, Alona

2016-01-01

Most ocean acidification (OA) experimental systems rely on pH as an indirect way to control CO2. However, accurate pH measurements are difficult to obtain and shifts in temperature and/or salinity alter the relationship between pH and pCO2. Here we describe a system in which the target pCO2 is controlled via direct analysis of pCO2 in seawater. This direct type of control accommodates potential temperature and salinity shifts, as the target variable is directly measured instead of being estimated. Water in a header tank is permanently re-circulated through an air-water equilibrator. The equilibrated air is then routed to an infrared gas analyzer (IRGA) that measures pCO2 and conveys this value to a Proportional-Integral-Derivative (PID) controller. The controller commands a solenoid valve that opens and closes the CO2 flush that is bubbled into the header tank. This low-cost control system allows the maintenance of stabilized levels of pCO2 for extended periods of time ensuring accurate experimental conditions. This system was used to study the long term effect of OA on the coralline red algae Phymatolithon lusitanicum. We found that after 11 months of high CO2 exposure, photosynthesis increased with CO2 as opposed to respiration, which was positively affected by temperature. Results showed that this system is adequate to run long-term OA experiments and can be easily adapted to test other relevant variables simultaneously with CO2, such as temperature, irradiance and nutrients. PMID:27703853

Impact of change in climate and policy from 1988 to 2007 on environmental and microbial variables at the time series station Boknis Eck, Baltic Sea

NASA Astrophysics Data System (ADS)

Hoppe, H.-G.; Giesenhagen, H. C.; Koppe, R.; Hansen, H.-P.; Gocke, K.

2013-07-01

Phytoplankton and bacteria are sensitive indicators of environmental change. The temporal development of these key organisms was monitored from 1988 to the end of 2007 at the time series station Boknis Eck in the western Baltic Sea. This period was characterized by the adaption of the Baltic Sea ecosystem to changes in the environmental conditions caused by the conversion of the political system in the southern and eastern border states, accompanied by the general effects of global climate change. Measured variables were chlorophyll, primary production, bacteria number, -biomass and -production, glucose turnover rate, macro-nutrients, pH, temperature and salinity. Negative trends with time were recorded for chlorophyll, bacteria number, bacterial biomass and bacterial production, nitrate, ammonia, phosphate, silicate, oxygen and salinity while temperature, pH, and the ratio between bacteria numbers and chlorophyll increased. Strongest reductions with time occurred for the annual maximum values, e.g. for chlorophyll during the spring bloom or for nitrate during winter, while the annual minimum values remained more stable. In deep water above sediment the negative trends of oxygen, nitrate, phosphate and bacterial variables as well as the positive trend of temperature were similar to those in the surface while the trends of salinity, ammonia and silicate were opposite to those in the surface. Decreasing oxygen, even in the surface layer, was of particular interest because it suggested enhanced recycling of nutrients from the deep hypoxic zones to the surface by vertical mixing. The long-term seasonal patterns of all variables correlated positively with temperature, except chlorophyll and salinity. Salinity correlated negatively with all bacterial variables (as well as precipitation) and positively with chlorophyll. Surprisingly, bacterial variables did not correlate with chlorophyll, which may be inherent with the time lag between the peaks of phytoplankton and bacteria during spring. Compared to the 20-yr averages of the environmental and microbial variables, the strongest negative deviations of corresponding annual averages were measured about ten years after political change for nitrate and bacterial secondary production (~ -60%), followed by chlorophyll (-50%) and bacterial biomass (-40%). Considering the circulation of surface currents in the Baltic Sea we interpret the observed patterns of the microbial variables at the Boknis Eck time series station as a consequence of the improved management of water resources after 1989 and - to a minor extent - the trends of the climate variables salinity and temperature.

Impact of change in climate and policy from 1988 to 2007 on environmental and microbial variables at the time series station Boknis Eck, Baltic Sea

NASA Astrophysics Data System (ADS)

Hoppe, H.-G.; Giesenhagen, H. C.; Koppe, R.; Hansen, H.-P.; Gocke, K.

2012-12-01

Phytoplankton and bacteria are sensitive indicators of environmental change. The temporal development of these key organisms was monitored from 1988 to the end of 2007 at the time series station Boknis Eck in the Western Baltic Sea. This period was characterized by the adaption of the Baltic Sea ecosystem to changes in the environmental conditions caused by the collapse and conversion of the political system in the Southern and Eastern Border States, accompanied by the general effects of global climate change. Measured variables were chlorophyll, primary production, bacteria number, -biomass and -production, glucose turnover rate, macro-nutrients, pH, temperature and salinity. Negative trends with time were recorded for chlorophyll, the bacterial variables, nitrate, ammonia, phosphate, silicate, oxygen and salinity while temperature, pH, and the ratio between bacteria numbers and chlorophyll increased. The strongest reductions with time occurred for the annual maximum values, e.g. for chlorophyll during the spring bloom or for nitrate during winter, while the annual minimum values remained more stable. In deep water above sediment the negative trends of oxygen, nitrate, phosphate and bacterial variables as well as the positive trend of temperature were similar to those in the surface while the trends of salinity, ammonia and silicate were opposite to those in the surface. Decreasing oxygen even in the surface layer was of particular interest because it suggested enhanced recycling of nutrients from the deep hypoxic zones to the surface by vertical mixing. In the long run all variables correlated positively with temperature, except chlorophyll and salinity. Salinity correlated negatively with all bacterial variables as well as precipitation and positively with chlorophyll. Surprisingly, bacterial variables did not correlate with chlorophyll which may be inherent with the time lag between the peaks of phytoplankton and bacteria during spring. Compared to the 20-yr averages of the environmental and microbial variables, the strongest negative deviations of corresponding annual averages were measured about ten years after political change for nitrate and bacterial secondary production (~ -60%), followed by chlorophyll (-50%) and bacterial biomass (-40%). Considering the circulation of surface currents in the Baltic Sea we conclude that the improved management of water resources after 1989 together with the trends of the climate variables salinity and temperature were responsible for the observed patterns of the microbial variables at the Boknis Eck time series station.

Decalcification and survival of benthic foraminifera under the combined impacts of varying pH and salinity.

PubMed

Charrieau, Laurie M; Filipsson, Helena L; Nagai, Yukiko; Kawada, Sachiko; Ljung, Karl; Kritzberg, Emma; Toyofuku, Takashi

2018-07-01

Coastal areas display natural large environmental variability such as frequent changes in salinity, pH, and carbonate chemistry. Anthropogenic impacts - especially ocean acidification - increase this variability, which may affect the living conditions of coastal species, particularly, calcifiers. We performed culture experiments on living benthic foraminifera to study the combined effects of lowered pH and salinity on the calcification abilities and survival of the coastal, calcitic species Ammonia sp. and Elphidium crispum. We found that in open ocean conditions (salinity ∼35) and lower pH than usual values for these species, the specimens displayed resistance to shell (test) dissolution for a longer time than in brackish conditions (salinity ∼5 to 20). However, the response was species specific as Ammonia sp. specimens survived longer than E. crispum specimens when placed in the same conditions of salinity and pH. Living, decalcified juveniles of Ammonia sp. were observed and we show that desalination is one cause for the decalcification. Finally, we highlight the ability of foraminifera to survive under Ω calc  < 1, and that high salinity and [Ca 2+ ] as building blocks are crucial for the foraminiferal calcification process. Copyright © 2018 Elsevier Ltd. All rights reserved.

Light induced changes of internal pH in a barnacle photoreceptor and the effect of internal pH on the receptor potential.

PubMed Central

Brown, H M; Meech, R W

1979-01-01

1. Intracellular pH (pH1) was measured in Balanus photoreceptors using pH-sensitive glass micro-electrodes. The average pH1 of twelve photoreceptors which had been dark adapted for at least 30 min was 7.3 +/- 0.07 (S.D.). 2. Illumination reduced the recorded pH1 by as much as 0.2 pH unit. The change in pH1 was graded with light intensity. 3. When the cells were exposed to CO2 in the dark, pH1 declined monophasically. Saline equilibrated with 2% CO2; 98% O2 produced a steady reduction in pH1 of about 0.25 unit in 2--3 min. The buffering capacity of the receptor cell cytoplasm calculated from such experiments is approximately 15 slykes. 4. In the presence of HCO3-1, CO2 saline produced smaller, biphasic changes in pH1. 5. The membrane depolarization produced by a bright flash (depolarizing receptor potential) was reversibly reduced in the presence of external CO2 or by injection of H+. Iontophoretic injection of HCO2- increased the amplitude of the receptor potential. 6. In individual cells there was a close correlation between the amplitude of the receptor potential and pH1. 7. Saline equilibrated with CO2 reduced the light induced current (recorded under voltage-clamp) by 40--50% without affecting its reversal potential. 8. Exposure of the receptor to 95% CO2 saline for several minutes (pH0 5.5) not only abolished the receptor potential but also reversibly decreased the K conductance of the membrane in the dark. These effects were not reproduced by pH0 5.5 buffered saline or by a 5 min exposure to saline equilibrated with N2. 9. It is suggested that changes in pH1 induced by light modulate the sensitivity of the receptor under physiological conditions. PMID:43890

Novel Natural Products from Extremophilic Fungi.

PubMed

Zhang, Xuan; Li, Shou-Jie; Li, Jin-Jie; Liang, Zi-Zhen; Zhao, Chang-Qi

2018-06-04

Extremophilic fungi have been found to develop unique defences to survive extremes of pressure, temperature, salinity, desiccation, and pH, leading to the biosynthesis of novel natural products with diverse biological activities. The present review focuses on new extremophilic fungal natural products published from 2005 to 2017, highlighting the chemical structures and their biological potential.

Trait-based framework to predict relative vulnerability of near-coastal species and habitats to climate change

EPA Science Inventory

Like Icarus, near-coastal species are “flying too close” to the sun, and are being impacted by climate-induced changes in air and ocean temperature, precipitation, salinity, ocean pH, and sea level rise. Sound management requires knowledge of what species and habitats...

Preparation of poly(acrylamide-co-acrylic acid)-grafted gum and its flocculation and biodegradation studies.

PubMed

Mittal, H; Mishra, Shivani B; Mishra, A K; Kaith, B S; Jindal, R; Kalia, S

2013-10-15

Biodegradation studies of Gum ghatti (Gg) and acrylamide-co-acrylic acid based flocculants [Gg-cl-poly(AAm-co-AA)] have been reported using the soil composting method. Gg-cl-poly(AAm-co-AA) was found to degrade 89.76% within 60 days. The progress of biodegradation at each stage was monitored through FT-IR and SEM. Polymer was synthesized under pressure using potassium persulphate-ascorbic acid as a redox initiator and N,N'-methylene-bis-acrylamide as a crosslinker. Synthesized polymer was found to show pH, temperature and ionic strength of the cations dependent swelling behavior. Gg-cl-poly(AAm-co-AA) was utilized for the selective absorption of saline from different petroleum fraction-saline emulsions. The flocculation efficiency of the polymer was studied as a function of polymer dose, temperature and pH of the solution. Gg-cl-poly(AAm-co-AA) showed maximum flocculation efficiency with 20 mol L(-1) polymer dose in acidic medium at 50 °C. Copyright © 2013. Published by Elsevier Ltd.

Laboratory study on coprecipitation of phosphate with ikaite in sea ice

NASA Astrophysics Data System (ADS)

Hu, Yu-Bin; Dieckmann, Gerhard S.; Wolf-Gladrow, Dieter A.; Nehrke, Gernot

2014-10-01

Ikaite (CaCO3·6H2O) has recently been discovered in sea ice, providing first direct evidence of CaCO3 precipitation in sea ice. However, the impact of ikaite precipitation on phosphate (PO4) concentration has not been considered so far. Experiments were set up at pH from 8.5 to 10.0, salinities from 0 to 105, temperatures from -4°C to 0°C, and PO4 concentrations from 5 to 50 µmol kg-1 in artificial sea ice brine so as to understand how ikaite precipitation affects the PO4 concentration in sea ice under different conditions. Our results show that PO4 is coprecipitated with ikaite under all experimental conditions. The amount of PO4 removed by ikaite precipitation increases with increasing pH. Changes in salinity (S ≥ 35) as well as temperature have little impact on PO4 removal by ikaite precipitation. The initial PO4 concentration affects the PO4 coprecipitation. These findings may shed some light on the observed variability of PO4 concentration in sea ice.

Chemical characteristics of the major thermal springs of Montana

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mariner, R.H.; Presser, T.S.; Evans, W.C.

1976-07-01

Twenty-one thermal springs in western Montana were sampled for chemical, isotope, and gas compositions. Most of the springs issue dilute to slightly saline sodium-bicarbonate waters of neutral to slightly alkaline pH. A few of the springs issue sodium-mixed anion waters of near neutral pH. Fluoride concentrations are high in most of the thermal waters, up to 18 miligrams per litre, while F/Cl ratios range from 3/1 in the dilute waters to 1/10 in the slightly saline waters. Most of the springs are theoretically in thermodynamic equilibrium with respect to calcite and fluorite. Nitrogen is the major gas escaping from mostmore » of the hot springs; however, Hunters Hot Springs issue principally methane. The deuterium content of the hot spring waters is typical of meteoric water in western Montana. Geothermal calculations based on silica concentrations and Na-K-Ca ratios indicate that most of the springs are associated with low temperature aquifers (less than 100/sup 0/C). Chalcedony may be controlling the silica concentrations in these low temperature aquifers even in ''granitic'' terranes.« less

Warming and surface ocean acidification over the last deglaciation: implications for foraminiferal assemblages

NASA Astrophysics Data System (ADS)

Dyez, K. A.; Hoenisch, B.; deMenocal, P. B.

2017-12-01

Although plankton drift with ocean currents, their presence and relative abundance varies across latitudes and environmental seawater conditions (e.g. temperature, pH, salinity). While earlier studies have focused on temperature as the primary factor for determining the regional species composition of planktic foraminiferal communities, evidence has recently been presented that foraminiferal shell thickness varies with ocean pH, and it remains unclear whether ongoing ocean acidification will cause ecological shifts within this plankton group. The transition from the last glacial maximum (LGM; 19,000-23,000 years B.P.) to the late Holocene (0-5,000 years B.P.) was characterized by both warming and acidification of the surface ocean, and thus provides an opportunity to study ecosystem shifts in response to these environmental changes. Here we provide new δ11B, Mg/Ca, and δ18O measurements from a suite of global sediment cores spanning this time range. We use these geochemical data to reconstruct ocean temperature, pH and salinity and pair the new data with previously published analyses of planktic foraminifera assemblages to study the respective effects of ocean warming and acidification on the foraminiferal habitat. At most open-ocean sample locations, our proxies indicate warming and acidification similar to previously published estimates, but in some marginal seas and coastal locations pH changes little between over the glacial termination. At face value, these observations suggest that warming is generally more important for ecosystem changes than acidification, at least over the slow rates of warming and ocean acidification in this time period. While geochemical data collection is being completed, we aim to include these data in an ecological model of foraminiferal habitat preferences.

Online PH measurement technique in seawater desalination

NASA Astrophysics Data System (ADS)

Wang, Haibo; Wu, Kaihua; Hu, Shaopeng

2009-11-01

The measurement technology of pH is essential in seawater desalination. Glass electrode is the main pH sensor in seawater desalination. Because the internal impedance of glass electrode is high and the signal of pH sensor is easy to be disturbed, a signal processing circuit with high input impedance was designed. Because of high salinity of seawater and the characteristic of glass electrode, ultrasonic cleaning technology was used to online clean pH sensor. Temperature compensation was also designed to reduce the measurement error caused by variety of environment temperature. Additionally, the potential drift of pH sensor was analyzed and an automatic calibration method was proposed. In order to online monitor the variety of pH in seawater desalination, three operating modes were designed. The three modes are online monitoring mode, ultrasonic cleaning mode and auto-calibration mode. The current pH in seawater desalination was measured and displayed in online monitoring mode. The cleaning process of pH sensor was done in ultrasonic cleaning mode. The calibration of pH sensor was finished in auto-calibration mode. The result of experiments showed that the measurement technology of pH could meet the technical requirements for desalination. The glass electrode could be promptly and online cleaned and its service life was lengthened greatly.

Microbial processes and factors controlling their activities in alkaline lakes of the Mongolian plateau

NASA Astrophysics Data System (ADS)

Namsaraev, Zorigto B.; Zaitseva, Svetlana V.; Gorlenko, Vladimir M.; Kozyreva, Ludmila P.; Namsaraev, Bair B.

2015-11-01

A striking feature of the Mongolian plateau is the wide range of air temperatures during a year, -30 to 30°C. High summer temperatures, atmospheric weathering and the arid climate lead to formation of numerous alkaline soda lakes that are covered by ice during 6-7 months per year. During the study period, the lakes had pH values between 8.1 to 10.4 and salinity between 1.8 and 360 g/L. According to chemical composition, the lakes belong to sodium carbonate, sodium chloride-carbonate and sodium sulfate-carbonate types. This paper presents the data on the water chemical composition, results of the determination of the rates of microbial processes in microbial mats and sediments in the lakes studied, and the results of a Principal Component Analysis of environmental variables and microbial activity data. Temperature was the most important factor that influenced both chemical composition and microbial activity. pH and salinity are also important factors for the microbial processes. Dark CO2 fixation is impacted mostly by salinity and the chemical composition of the lake water. Total photosynthesis and sulfate-reduction are impacted mostly by pH. Photosynthesis is the dominant process of primary production, but the highest rate (386 mg C/(L•d)) determined in the lakes studied were 2-3 times lower than in microbial mats of lakes located in tropical zones. This can be explained by the relatively short warm period that lasts only 3-4 months per year. The highest measured rate of dark CO2 assimilation (59.8 mg C/(L•d)) was much lower than photosynthesis. The highest rate of sulfate reduction was 60 mg S/(L•d), while that of methanogenesis was 75.6 μL CN4/(L•d) in the alkaline lakes of Mongolian plateau. The rate of organic matter consumption during sulfate reduction was 3-4 orders of magnitude higher than that associated with methanogenesis.

Bacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditions

PubMed Central

Scarascia, Giantommaso; Yap, Scott A.; Kaksonen, Anna H.; Hong, Pei-Ying

2018-01-01

Pseudomonas aeruginosa is a ubiquitous member of marine biofilm, and reduces thiosulfate to produce toxic hydrogen sulfide gas. In this study, lytic bacteriophages were isolated and applied to inhibit the growth of P. aeruginosa in planktonic mode at different temperature, pH, and salinity. Bacteriophages showed optimal infectivity at a multiplicity of infection of 10 in saline conditions, and demonstrated lytic abilities over all tested temperature (25, 30, 37, and 45°C) and pH 6–9. Planktonic P. aeruginosa exhibited significantly longer lag phase and lower specific growth rates upon exposure to bacteriophages. Bacteriophages were subsequently applied to P. aeruginosa-enriched biofilm and were determined to lower the relative abundance of Pseudomonas-related taxa from 0.17 to 5.58% in controls to 0.01–0.61% in treated microbial communities. The relative abundance of Alphaproteobacteria, Pseudoalteromonas, and Planococcaceae decreased, possibly due to the phage-induced disruption of the biofilm matrix. Lastly, when applied to mitigate biofouling of ultrafiltration membranes, bacteriophages were determined to reduce the transmembrane pressure increase by 18% when utilized alone, and by 49% when used in combination with citric acid. The combined treatment was more effective compared with the citric acid treatment alone, which reported ca. 30% transmembrane pressure reduction. Collectively, the findings demonstrated that bacteriophages can be used as a biocidal agent to mitigate undesirable P. aeruginosa-associated problems in seawater applications. PMID:29770130

[Oxygen consumption rate of Sepia pharaonis embryos.

PubMed

Wang, Peng Shuai; Jiang, Xia Min; Ruan, Peng; Peng, Rui Bing; Jiang, Mao Wang; Han, Qing Xi

2016-07-01

This research was conducted to unravel the variation of oxygen consumption rate during different developmental stages and the effects of different ecological factors on embryonic oxygen consumption rate of Sepia pharaonis. The oxygen consumption rates were measured at twelve deve-lopmental stages by the sealed volumetric flasks, and four embryonic developmental periods (oosperm, gastrula, the formation of organization, endoskeleton) were selected under various ecological conditions, such as salinity (21, 24, 27, 30, 33), water temperature (18, 21, 24, 27, 30 ℃) and pH (7.0, 7.5, 8.0, 8.5, 9.0). The results showed that the oxygen consumption rate rose along with the developmental progress, and distinctly differed from each other. The oxygen consumption rate was 0.082 mg·(100 eggs) -1 ·h -1 during oosperm period, and rose to 0.279 mg·(100 eggs) -1 ·h -1 during gastrula period, which was significantly higher than that of blastula period. Finally, the oxygen consumption rate rose to 1.367 mg·(100 eggs) -1 ·h -1 during hatching period. The salinity showed a significant effect on oxygen consumption rate during the formation of organization and endoskeleton formation stage (P<0.05), but no significant effect during oosperm and gastrula periods (P>0.05). The oxygen consumption rates of four studied embryonic stages all rose and then declined along with the increase of salinity, and reached the highest values [0.082, 0.200, 0.768 and 1.301 mg·(100 eggs) -1 ·h -1 , respectively] at salinity 30. The water temperature had a significant effect on the embryo oxygen consumption rates of gastrula, and the formation of organization and endoskeleton formation stage (P<0.05), with the exception of oosperm (P>0.05). The oxygen consumption rates of four studied embryonic stages all rose and then declined along with the increase of temperature, and reached the highest values at 27 ℃ [0.082, 0.286, 0.806 and 1.338 mg·(100 eggs) -1 ·h -1 , respectively]. The pH had no significant effect on the oxygen consumption rates of four embryonic stages (P>0.05). The oxygen consumption rates of four studied embryonic stages all rose and then declined along with the increase of pH. The oxygen consumption rates of gastrula, the formation of organization, endoskeleton reached the according highest values [0.281, 0.799 and 1.130 mg·(100 eggs) -1 ·h -1 ] at pH 8.5, but that during oosperm period occurred at pH 8.0 [0.116 mg·(100 eggs) -1 ·h -1 ].

Erosion and attrition of human enamel in vitro part I: interaction effects.

PubMed

Eisenburger, M; Addy, M

2002-01-01

The aim of the study in vitro was to measure the interplay of attrition and erosion of human enamel under several different pH conditions. Cusp and smooth surface enamel specimens were prepared from unerupted human third molar teeth. Cusp and smooth surface specimens opposed each other in a wear machine under a load of 600 g. The machine simulated tooth grinding for 30 min (2280 wear cycles). Six groups of 10 specimens were worn in the following environmental conditions: saline, citric acid at pH values of 3.2, 5.5 or 7.0 and two cycling groups (pH 3.2/saline or saline/pH 3.2). Additionally, 10 smooth surface specimens were exposed to the same fluids without attrition. Tissue loss on smooth surface specimens was determined by profilometry. The worn specimen surfaces were studied by SEM. Attrition depth in saline and pH 7.0 citric acid was 25.5 microm. At pH 3.2 and 5.5 attrition depths were 6.0 and 4.3 microm, respectively. Cycling in saline/citric acid and vice versa produced attrition depths of 9.2 and 7.9 microm, respectively. In the erosion only specimens lesion depths were 3.0, 1.2 and 0 microm at pH 3.2, 5.5 and 7.0 and saline, respectively. Attrition specimens at pH 7.0 and in saline showed very rough surfaces. At low pH enamel surfaces appeared flat and smooth. Enamel wear in neutral conditions is significantly higher than in acidic conditions (p < 0.05), apparently due to a smoothing effect of erosion on contacting surfaces. Copyright 2002 Elsevier Science Ltd.

Halophilic Bacteria as a Source of Novel Hydrolytic Enzymes

PubMed Central

de Lourdes Moreno, María; Pérez, Dolores; García, María Teresa; Mellado, Encarnación

2013-01-01

Hydrolases constitute a class of enzymes widely distributed in nature from bacteria to higher eukaryotes. The halotolerance of many enzymes derived from halophilic bacteria can be exploited wherever enzymatic transformations are required to function under physical and chemical conditions, such as in the presence of organic solvents and extremes in temperature and salt content. In recent years, different screening programs have been performed in saline habitats in order to isolate and characterize novel enzymatic activities with different properties to those of conventional enzymes. Several halophilic hydrolases have been described, including amylases, lipases and proteases, and then used for biotechnological applications. Moreover, the discovery of biopolymer-degrading enzymes offers a new solution for the treatment of oilfield waste, where high temperature and salinity are typically found, while providing valuable information about heterotrophic processes in saline environments. In this work, we describe the results obtained in different screening programs specially focused on the diversity of halophiles showing hydrolytic activities in saline and hypersaline habitats, including the description of enzymes with special biochemical properties. The intracellular lipolytic enzyme LipBL, produced by the moderately halophilic bacterium Marinobacter lipolyticus, showed advantages over other lipases, being an enzyme active over a wide range of pH values and temperatures. The immobilized LipBL derivatives obtained and tested in regio- and enantioselective reactions, showed an excellent behavior in the production of free polyunsaturated fatty acids (PUFAs). On the other hand, the extremely halophilic bacterium, Salicola marasensis sp. IC10 showing lipase and protease activities, was studied for its ability to produce promising enzymes in terms of its resistance to temperature and salinity. PMID:25371331

Distribution of hydro-biological parameters in coastal waters off Rushikulya Estuary, East Coast of India: a premonsoon case study.

PubMed

Baliarsingh, S K; Srichandan, S; Naik, S; Sahu, K C; Lotliker, Aneesh A; Kumar, T S

2013-08-15

The hydro-biological parameters of coastal waters off Rushikulya estuary was investigated during premonsoon 2011. Important hydro-biological parameters such as water temperature, salinity, pH, DO, NO2, NO3, NH4, PO4, SiO4, TSM, Chl-a, phytoplankton and zooplankton were measured during the present study. Temperature established a strong positive correlation with salinity and pH during the present study. Chl-a found in positive relation with NO3, SiO, and TSM. Analysis of variance revealed significant monthly variation in pH, salinity and TSM. Significant station wise variation was observed in DO and most of the nutrients i.e., NO3, NH4, PO4, SiO4. A total of 119 species of phytoplankton were identified of which 84 species are of diatoms, 22 species of dinoflagellates, 7 species of green algae, 5 species of cyanobacteria (blue green algae) and 1 species of cocolithophore. Phytoplankton abundance varied between 25543 (Nos. L(-1)) and 36309 (Nos. L(-1)). Diatoms dominated the phytoplankton community followed by dinoflagellates in all the months. Diatoms contributed to 82-89% of the total phytoplankton population density whereas dinoflagellates contributed to 6-12%. The regression between Chl-a and phytoplankton abundance resulted with weak relation (R(2) = 0.042). Zooplankton fauna composed of 134 species of holoplankton and 20 types of meroplankton were encountered during the study period. Zooplankton population dominated by copepod during all months and accounted for 74 to 85% to the total zooplankton. The population density ranged from 6959 to 35869 Nos./10 m(3). Analysis of variance explained no significant variation in total zooplankton abundance and also for different groups of zooplankton.

Correlations between Microbial Indicators, Pathogens, and Environmental Factors in a Subtropical Estuary

PubMed Central

Ortega, Cristina; Solo-Gabriele, Helena M.; Abdelzaher, Amir; Wright, Mary; Deng, Yang; Stark, Lillian M.

2009-01-01

The objective of this study was to evaluate whether indicator microbes and physical-chemical parameters were correlated with pathogens within a tidally influenced estuary. Measurements included the analysis of physical-chemical parameters (pH, salinity, temperature, and turbidity), measurements of bacterial indicators (enterococci, fecal coliform, E. coli, and total coliform), viral indicators (somatic and MS2 coliphage), viral pathogens (enterovirus by culture), and protozoan pathogens (Cryptosporidium and Giardia). All pathogen results were negative with the exception of one sample which tested positive for culturable reovirus (8.5 MPN/100 L).. Notable physical-chemical parameters for this sample included low salinity (<1 ppt) and high water temperature (31 °C). Indicator bacteria and indicator virus levels for this sample were within average values typically measured within the study site and were low in comparison with levels observed in other freshwater environments. Overall results suggest that high levels of bacterial and viral indicators were associated with low salinity sites. PMID:19464704

A chemical model of seawater including dissolved ammonia and the stoichiometric dissociation constant of ammonia in estuarine water and seawater from -2 to 40°C

NASA Astrophysics Data System (ADS)

Clegg, Simon L.; Whitfield, Michael

1995-06-01

The calculation of the percentage of un-ionised ammonia in estuarine water and seawater requires values of the stoichiometric dissociation constant of ammonia, defined by: K*a/mol kg -1 = mNH 3mH +/ mNH +4, where m denotes molality. A thermodynamic model of seawater, including dissolved NH 3 and NH +4, is developed using an extended Pitzer formalism parameterised from available data. The model is validated using emf measurements for cells containing artificial seawater with added HCl, and NH 4Cl, and NH 3 over a range of temperatures and salinities. Calculated values of K*a are tabulated from 0 to 40 ppt salinity and -2 to 40°C, on both a free ( mH +) and total ( mH + + mHSO -4) hydrogen ion basis for use with pH measurements made on the corresponding scales. Accuracy (in K*a) is likely to be better than 5% at all temperatures and salinities.

Soil Respiration in Different Agricultural and Natural Ecosystems in an Arid Region

PubMed Central

Lai, Liming; Zhao, Xuechun; Jiang, Lianhe; Wang, Yongji; Luo, Liangguo; Zheng, Yuanrun; Chen, Xi; Rimmington, Glyn M.

2012-01-01

The variation of different ecosystems on the terrestrial carbon balance is predicted to be large. We investigated a typical arid region with widespread saline/alkaline soils, and evaluated soil respiration of different agricultural and natural ecosystems. Soil respiration for five ecosystems together with soil temperature, soil moisture, soil pH, soil electric conductivity and soil organic carbon content were investigated in the field. Comparing with the natural ecosystems, the mean seasonal soil respiration rates of the agricultural ecosystems were 96%–386% higher and agricultural ecosystems exhibited lower CO2 absorption by the saline/alkaline soil. Soil temperature and moisture together explained 48%, 86%, 84%, 54% and 54% of the seasonal variations of soil respiration in the five ecosystems, respectively. There was a significant negative relationship between soil respiration and soil electrical conductivity, but a weak correlation between soil respiration and soil pH or soil organic carbon content. Our results showed that soil CO2 emissions were significantly different among different agricultural and natural ecosystems, although we caution that this was an observational, not manipulative, study. Temperature at the soil surface and electric conductivity were the main driving factors of soil respiration across the five ecosystems. Care should be taken when converting native vegetation into cropland from the point of view of greenhouse gas emissions. PMID:23082234

Soil respiration in different agricultural and natural ecosystems in an arid region.

PubMed

Lai, Liming; Zhao, Xuechun; Jiang, Lianhe; Wang, Yongji; Luo, Liangguo; Zheng, Yuanrun; Chen, Xi; Rimmington, Glyn M

2012-01-01

The variation of different ecosystems on the terrestrial carbon balance is predicted to be large. We investigated a typical arid region with widespread saline/alkaline soils, and evaluated soil respiration of different agricultural and natural ecosystems. Soil respiration for five ecosystems together with soil temperature, soil moisture, soil pH, soil electric conductivity and soil organic carbon content were investigated in the field. Comparing with the natural ecosystems, the mean seasonal soil respiration rates of the agricultural ecosystems were 96%-386% higher and agricultural ecosystems exhibited lower CO(2) absorption by the saline/alkaline soil. Soil temperature and moisture together explained 48%, 86%, 84%, 54% and 54% of the seasonal variations of soil respiration in the five ecosystems, respectively. There was a significant negative relationship between soil respiration and soil electrical conductivity, but a weak correlation between soil respiration and soil pH or soil organic carbon content. Our results showed that soil CO(2) emissions were significantly different among different agricultural and natural ecosystems, although we caution that this was an observational, not manipulative, study. Temperature at the soil surface and electric conductivity were the main driving factors of soil respiration across the five ecosystems. Care should be taken when converting native vegetation into cropland from the point of view of greenhouse gas emissions.

Development of an underwater in-situ spectrophotometric sensor for seawater pH

NASA Astrophysics Data System (ADS)

Waterbury, Robert D.; Byrne, Robert H.; Kelly, John; Leader, Bram; McElligott, Sean; Russell, Randy

1996-12-01

A pH sensor based upon spectrophotometric techniques has been developed for in-situ analysis of surface seawater. This sensor utilizes a spectrophotometric pH indicator (Thymol Blue) which has been calibrated for use in seawater as a function of temperature and salinity. Shipboard spectrophotometric pH analyses routinely demonstrate a precision on the order of plus or minus 0.0004 pH units. In- situ analysis of seawater pH has demonstrated a precision on the order of plus or minus 0.001 and an accuracy, using shipboard measurements as a standard, on the order of plus or minus 0.01. The sensor is a self-contained system which pumps seawater, meters in indicator, spectrophotometrically determines indicator absorbance and stores data with a 1 Hz acquisition frequency. The sensor employs two absorbance cells, each with three wavelength channels, to obtain the spectrophotometric absorbance. The sensor system, rated for depths up to 500 m, provides pH, conductivity, temperature and can be operated via computer or in a standalone mode with internal data storage. The sensor utilizes less than 12 watts of power and is packaged in a 29' long by 4.5' diameter aluminum housing.

Continuous water-quality monitoring to improve lake management at Lake Mattamuskeet National Wildlife Refuge

Treesearch

Michelle Moorman; Tom Augspurger

2016-01-01

The U.S. Fish and Wildlife Service has partnered with U.S. Geological Survey to establish 2 continuous water-quality monitoring stations at Lake Mattamuskeet. Stations on the east and west side of the lake measure water level, clarity, dissolved oxygen, pH, temperature, salinity, and conductivity.

Empirical algorithms to estimate water column pH in the Southern Ocean

NASA Astrophysics Data System (ADS)

Williams, N. L.; Juranek, L. W.; Johnson, K. S.; Feely, R. A.; Riser, S. C.; Talley, L. D.; Russell, J. L.; Sarmiento, J. L.; Wanninkhof, R.

2016-04-01

Empirical algorithms are developed using high-quality GO-SHIP hydrographic measurements of commonly measured parameters (temperature, salinity, pressure, nitrate, and oxygen) that estimate pH in the Pacific sector of the Southern Ocean. The coefficients of determination, R2, are 0.98 for pH from nitrate (pHN) and 0.97 for pH from oxygen (pHOx) with RMS errors of 0.010 and 0.008, respectively. These algorithms are applied to Southern Ocean Carbon and Climate Observations and Modeling (SOCCOM) biogeochemical profiling floats, which include novel sensors (pH, nitrate, oxygen, fluorescence, and backscatter). These algorithms are used to estimate pH on floats with no pH sensors and to validate and adjust pH sensor data from floats with pH sensors. The adjusted float data provide, for the first time, seasonal cycles in surface pH on weekly resolution that range from 0.05 to 0.08 on weekly resolution for the Pacific sector of the Southern Ocean.

Evaluating Carbonate System Algorithms in a Nearshore System: Does Total Alkalinity Matter?

PubMed Central

Sweet, Julia; Brzezinski, Mark A.; McNair, Heather M.; Passow, Uta

2016-01-01

Ocean acidification is a threat to many marine organisms, especially those that use calcium carbonate to form their shells and skeletons. The ability to accurately measure the carbonate system is the first step in characterizing the drivers behind this threat. Due to logistical realities, regular carbonate system sampling is not possible in many nearshore ocean habitats, particularly in remote, difficult-to-access locations. The ability to autonomously measure the carbonate system in situ relieves many of the logistical challenges; however, it is not always possible to measure the two required carbonate parameters autonomously. Observed relationships between sea surface salinity and total alkalinity can frequently provide a second carbonate parameter thus allowing for the calculation of the entire carbonate system. Here, we assessed the rigor of estimating total alkalinity from salinity at a depth <15 m by routinely sampling water from a pier in southern California for several carbonate system parameters. Carbonate system parameters based on measured values were compared with those based on estimated TA values. Total alkalinity was not predictable from salinity or from a combination of salinity and temperature at this site. However, dissolved inorganic carbon and the calcium carbonate saturation state of these nearshore surface waters could both be estimated within on average 5% of measured values using measured pH and salinity-derived or regionally averaged total alkalinity. Thus we find that the autonomous measurement of pH and salinity can be used to monitor trends in coastal changes in DIC and saturation state and be a useful method for high-frequency, long-term monitoring of ocean acidification. PMID:27893739

Evaluating Carbonate System Algorithms in a Nearshore System: Does Total Alkalinity Matter?

PubMed

Jones, Jonathan M; Sweet, Julia; Brzezinski, Mark A; McNair, Heather M; Passow, Uta

2016-01-01

Ocean acidification is a threat to many marine organisms, especially those that use calcium carbonate to form their shells and skeletons. The ability to accurately measure the carbonate system is the first step in characterizing the drivers behind this threat. Due to logistical realities, regular carbonate system sampling is not possible in many nearshore ocean habitats, particularly in remote, difficult-to-access locations. The ability to autonomously measure the carbonate system in situ relieves many of the logistical challenges; however, it is not always possible to measure the two required carbonate parameters autonomously. Observed relationships between sea surface salinity and total alkalinity can frequently provide a second carbonate parameter thus allowing for the calculation of the entire carbonate system. Here, we assessed the rigor of estimating total alkalinity from salinity at a depth <15 m by routinely sampling water from a pier in southern California for several carbonate system parameters. Carbonate system parameters based on measured values were compared with those based on estimated TA values. Total alkalinity was not predictable from salinity or from a combination of salinity and temperature at this site. However, dissolved inorganic carbon and the calcium carbonate saturation state of these nearshore surface waters could both be estimated within on average 5% of measured values using measured pH and salinity-derived or regionally averaged total alkalinity. Thus we find that the autonomous measurement of pH and salinity can be used to monitor trends in coastal changes in DIC and saturation state and be a useful method for high-frequency, long-term monitoring of ocean acidification.

Modeling coliform-bacteria concentrations and pH in the salt-wedge reach of the Duwamish River Estuary, King County, Washington

USGS Publications Warehouse

Haushild, W.L.; Prych, Edmund A.

1976-01-01

Total- and fecal-coliform bacteria, plus pH, alkalinity, and dissolved inorganic carbon are water-quality parameters that have been added to an existing numerical model of water quality in the salt-wedge reach of the Duwamish River estuary in Washington. The coliform bacteria are modeled using a first-order decay (death) rate, which is a function of the local salinity, temperature, and daily solar radiation. The pH is computed by solving a set of chemical-equilibrium equations for carbonate-bicarbonate buffered aqueous solutions. Concentrations of total- and fecal-coliform bacteria computed by the model for the Duwamish River estuary during June-September 1971 generally agreed with observed concentrations within about 40 and 60 percent, respectively. The computed pH generally agreed with observed pH within about a 0.2 pH unit; however, for one 3-week period the computed pH was about a 0.4 unit lower than the observed pH. (Woodard-USGS)

pH dependent salinity-boron interactions impact yield, biomass, evapotranspiration and boron uptake in broccoli (Brassica oleracea L.)

USDA-ARS?s Scientific Manuscript database

Soil pH is known to influence many important biochemical processes in plants and soils, however its role in salinity - boron interactions affecting plant growth and ion relations has not been examined. The purpose of this research was to evaluate the interactive effects of salinity, boron and soil ...

Cruise Summary of WHP P6, A10, I3 and I4 Revisits in 2003

NASA Astrophysics Data System (ADS)

Kawano, T.; Uchida, H.; Schneider, W.; Kumamoto, Y.; Nishina, A.; Aoyama, M.; Murata, A.; Sasaki, K.; Yoshikawa, Y.; Watanabe, S.; Fukasawa, M.

2004-12-01

Japan Agency for Marin-Earth Science and Technology (JAMSTEC) conducted a research cruise to round in the southern hemisphere by R/V Mirai. In this presentation, we introduce an outline of the cruise and data quality obtained during the cruise. The cruise started on Aug. 3, 2003 in Brisbane, Australia and sailed eastward until it reached Fremantle, Australia on Feb. 19, 2004. It contained six legs and legs 1, 2, 4 and 5 were revisits of WOCE Hydrographic Program (WHP) sections P6W, P6E, A10 and I3/I4, respectively. The sections consisted of about 500 hydrographic stations in total. On each station, CTD profiles and up to 36 water samples by 12L Niskin-X bottles were taken from the surface to within 10 m of the bottom. Water samples were analyzed at every station for salinity, dissolved oxygen (DO), and nutrients and at alternate stations for concentration of freons, dissolved inorganic carbon (CT), total alkalinity (AT), pH, and so on. Approximately 17,000 samples were obtained for salinity. The standard seawater was measured repeatedly to estimate the uncertainty caused by the setting and stability of the salinometer. The standard deviation of 699 repeated runs of standard seawater was 0.0002 in salinity. Replicate samples, which are a pair of samples drawn from the same Niskin bottle to different sample bottles, were taken to evaluate the overall uncertainty. The standard deviation of absolute differences of 2,769 replicates was also 0.0002 in salinity. For DO, about 13,400 samples were obtained. The analysis was made by a photometric titration technique. The reproducibility estimated from the absolute standard deviation of 1,625 replicates was about 0.09 umol/kg. CTD temperature was calibrated against a deep ocean standards thermometer (SBE35) which was attached to the CTD using a polynomial expression Tcal = T - (a +b*P + c*t), where Tcal is calibrated temperature, T is CTD temperature, P is CTD pressure and t is time. Calibration coefficients, a, b and c, were determined for each station by minimizing the sum of absolute deviation from SBE35 temperature below 2,000dbar. CTD salinity and DO were fitted to values obtained by sampled water analysis using similar polynomials. These corrections yielded deviations of about 0.0002 K in temperature, 0.0003 in salinity and 0.6 umol/kg in DO. Nutrients analyses were accomplished on 16,000 samples using the reference material of nutrients in seawater (RMNS). To establish the traceability and to get higher quality data, 500 bottles of RMNS from the same lot and 150 sets of RMNSs were used. The precisions of phosphate, nitrate and silicate measurements were 0.18 %, 0.17 % and 0.16 % in terms of median of those at 493 stations, respectively. The nutrients concentrations could be expressed with uncertainties explicitly because of the repeated runs of RMNSs. All the analyses for the CO{2}-system parameters in water columns were finished onboard. Analytical precisions of CT, AT and pH were estimated to be \\sim1.0 umol/kg, \\sim2.0 umol/kg, and \\sim7*10-4 pH unit, respectively. Approximately 6,300 samples were obtained for CFC-11 and CFC-12. The concentrations were determined with an electron capture detector - gas chromatograph (ECD-GC) attached the purge and trapping system. The reproducibility estimated from the absolute standard deviation of 365 replicates was less than 1% with respect to the surface concentrations.

Erratum to ;Coastal water column ammonium and nitrite oxidation are decoupled in summer;

NASA Astrophysics Data System (ADS)

Heiss, Elise M.; Fulweiler, Robinson W.

2017-07-01

Water column nitrification is a key process in the nitrogen cycle as it links reduced and oxidized forms of nitrogen and also provides the substrate (nitrate) needed for reactive nitrogen removal by denitrification. We measured potential water column ammonium and nitrite oxidation rates at four sites along an estuary to continental shelf gradient over two summers. In most cases, nitrite oxidation rates outpaced ammonium oxidation rates. Overall, ammonium and nitrite oxidation rates were higher outside of the estuary, and this trend was primarily driven by higher oxidation rates in deeper waters. Additionally, both ammonium and nitrite oxidation rates were impacted by different in situ variables. Ammonium oxidation rates throughout the water column as a whole were most positively correlated to depth and salinity and negatively correlated to dissolved oxygen, light, and temperature. In contrast, nitrite oxidation rates throughout the water column were negatively correlated with temperature, light and pH. Multivariate regression analysis revealed that surface (<20 m) ammonium oxidation rates were most strongly predicted by substrate (NH4+), salinity, and light, while deep (>20 m) rates were regulated by temperature, light, and [H+] (i.e. pH). In addition, surface (<20 m) nitrite oxidation rates were best explained by [H+] alone, while [H+], temperature, and dissolved oxygen all played a role in predicting deep (>20 m) nitrite oxidation rates. These results support the growing body of evidence that ammonium oxidation and nitrite oxidation are not always coupled, should be measured separately, and are influenced by different environmental conditions.

Repurposing tromethamine as inhaled therapy to treat CF airway disease

PubMed Central

Alaiwa, Mahmoud H. Abou; Launspach, Janice L.; Sheets, Kelsey A.; Rivera, Jade A.; Gansemer, Nicholas D.; Taft, Peter J.; Thorne, Peter S.; Welsh, Michael J.; Stoltz, David A.

2016-01-01

In cystic fibrosis (CF), loss of CF transmembrane conductance regulator (CFTR) anion channel activity causes airway surface liquid (ASL) pH to become acidic, which impairs airway host defenses. One potential therapeutic approach is to correct the acidic pH in CF airways by aerosolizing HCO3– and/or nonbicarbonate pH buffers. Here, we show that raising ASL pH with inhaled HCO3– increased pH. However, the effect was transient, and pH returned to baseline values within 30 minutes. Tromethamine (Tham) is a buffer with a long serum half-life used as an i.v. formulation to treat metabolic acidosis. We found that Tham aerosols increased ASL pH in vivo for at least 2 hours and enhanced bacterial killing. Inhaled hypertonic saline (7% NaCl) is delivered to people with CF in an attempt to promote mucus clearance. Because an increased ionic strength inhibits ASL antimicrobial factors, we added Tham to hypertonic saline and applied it to CF sputum. We found that Tham alone and in combination with hypertonic saline increased pH and enhanced bacterial killing. These findings suggest that aerosolizing the HCO3–-independent buffer Tham, either alone or in combination with hypertonic saline, might be of therapeutic benefit in CF airway disease. PMID:27390778

Environmental parameters influence on the dynamics of total and pathogenic Vibrio parahaemolyticus densities in Crassostrea virginica harvested from Mexico's Gulf coast.

PubMed

López-Hernández, Karla M; Pardío-Sedas, Violeta T; Lizárraga-Partida, Leonardo; Williams, José de J; Martínez-Herrera, David; Flores-Primo, Argel; Uscanga-Serrano, Roxana; Rendón-Castro, Karla

2015-02-15

The influence of environmental parameters on the total and pathogenic Vibrio parahaemolyticus seasonal densities in American oysters (Crassostrea virginica) was evaluated for 1 year. Harvesting site A yielded the highest mean densities of V. parahaemolyticus tlh+, tdh+/trh-, tdh-/trh+ and tdh+/trh+ during spring season at 2.57, 1.74, 0.36, and -0.40 log10 MPN/g, respectively, and tdh+/orf8+ during winter season (0.90 log10 MPN/g). V. parahaemolyticus tlh+ densities were associated to salinity (R(2)=0.372, P<0.022), tdh+/trh+ to turbidity (R(2)=0.597, P<0.035), and orf8+ to temperature, salinity, and pH (R(2)=0.964, P<0.001). The exposure to salinity and temperature conditions during winter and spring seasons regulated the dynamics of V. parahaemolyticus harboring potentially pathogenic genotypes within the oyster. The adaptive response of V. parahaemolyticus to seasonal environmental changes may lead to an increase in survival and virulence, threatening the seafood safety and increasing the risk of illness. Copyright © 2014 Elsevier Ltd. All rights reserved.

Electrocapillary Phenomena at Edible Oil/Saline Interfaces.

PubMed

Nishimura, Satoshi; Ohzono, Takuya; Shoji, Kohei; Yagihara, Shin; Hayashi, Masafumi; Tanaka, Hisao

2017-03-01

Interfacial tension between edible oil and saline was measured under applied electric fields to understand the electrocapillary phenomena at the edible oil/saline interfaces. The electric responses of saline droplets in edible oil were also observed microscopically to examine the relationship between the electrocapillary phenomena and interfacial polarization. When sodium oleate (SO) was added to edible oil (SO-oil), the interfacial tension between SO-oil and saline decreased. However, no decrease was observed for additive-free oil or oleic acid (OA)-added oil (OA-oil). Microscopic observations suggested that the magnitude of interfacial polarization increased in the order of additive-free oil < OA-oil < SO-oil. The difference in electrocapillary phenomena between OA- and SO-oils was closely related to the polarization magnitude. In the case of SO-oil, the decrease in interfacial tension was remarkably larger for saline (pH 5.4~5.6) than that for phosphate-buffered saline (PBS, pH 7.2~7.4). However, no difference was observed between the electric responses of PBS and saline droplets in SO-oil. The difference in electrocapillary phenomena for PBS and saline could not be simply explained in terms of polarization magnitude. The ratio of ionized and non-ionized OA at the interfaces changed with the saline pH, possibly leading to the above difference.

Review of Dredging Elutriate Application Factors: Relevance to Acute-to-Chronic Protection, Contaminant, and Endpoint Specificity

DTIC Science & Technology

2015-07-01

Postma et al. 2002). Unionized ammonia (NH3) is more toxic relative to the ammonium ion ( NH4 +) (USEPA 1989, 1999a, 2009). The fraction of total...ammonia (i.e., sum concentration of NH4 + and NH3) that is unionized ammonia (UIA) is contingent on the pH, temperature, and salinity of the test water

Weaving Common Threads: Reaching out to American Indians and Native Peoples of Alaska and the Pacific Islands

NASA Technical Reports Server (NTRS)

2002-01-01

This report outlines the activities of the GLOBE (Global Learning and Observations to Benefit the Environment) Train-the-Trainer Workshop. Educators were introduced to the GLOBE protocols for Atmosphere, Hydrology, Soil and Land Cover. These protocols included measurement of pH, temperature, precipitation, salinity, and soil moisture content. Each topic included implementation plans and learning activities.

Ocean acidification alters temperature and salinity preferences in larval fish.

PubMed

Pistevos, Jennifer C A; Nagelkerken, Ivan; Rossi, Tullio; Connell, Sean D

2017-02-01

Ocean acidification alters the way in which animals perceive and respond to their world by affecting a variety of senses such as audition, olfaction, vision and pH sensing. Marine species rely on other senses as well, but we know little of how these might be affected by ocean acidification. We tested whether ocean acidification can alter the preference for physicochemical cues used for dispersal between ocean and estuarine environments. We experimentally assessed the behavioural response of a larval fish (Lates calcarifer) to elevated temperature and reduced salinity, including estuarine water of multiple cues for detecting settlement habitat. Larval fish raised under elevated CO 2 concentrations were attracted by warmer water, but temperature had no effect on fish raised in contemporary CO 2 concentrations. In contrast, contemporary larvae were deterred by lower salinity water, where CO 2 -treated fish showed no such response. Natural estuarine water-of higher temperature, lower salinity, and containing estuarine olfactory cues-was only preferred by fish treated under forecasted high CO 2 conditions. We show for the first time that attraction by larval fish towards physicochemical cues can be altered by ocean acidification. Such alterations to perception and evaluation of environmental cues during the critical process of dispersal can potentially have implications for ensuing recruitment and population replenishment. Our study not only shows that freshwater species that spend part of their life cycle in the ocean might also be affected by ocean acidification, but that behavioural responses towards key physicochemical cues can also be negated through elevated CO 2 from human emissions.

Preference of multi-walled carbon nanotube (MWCNT) to single-walled carbon nanotube (SWCNT) and activated carbon for preparing silica nanohybrid pickering emulsion for chemical enhanced oil recovery (C-EOR)

DOE Office of Scientific and Technical Information (OSTI.GOV)

AfzaliTabar, M.; Alaei, M., E-mail: alaiem@ripi.ir; Ranjineh Khojasteh, R.

The aim of this research was to determine the best nano hybrid that can be used as a Pickering emulsion Chemical Enhanced Oil Recovery (C-EOR). Therefore, we have prepared different carbon structures nano hybrids with SiO{sub 2} nano particles with different weight percent using sol-gel method. The as-prepared nano materials were characterized with X-Ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FE-SEM) and Thermal Gravimetric Analysis (TGA). Pickering emulsions of these nanohybrids were prepared at pH=7 in ambient temperature and with distilled water. Stability of the mentioned Pickering emulsions was controlled for one month. Emulsion phase morphology was investigated usingmore » optical microscopic imaging. Evaluation results demonstrated that the best sample is the 70% MWCNT/SiO{sub 2} nanohybrid. Stability of the selected nanohybrid (70% MWCNT/SiO{sub 2} nanohybrid) was investigated by alteration of salinity, pH and temperature. Results showed that the mentioned Pickering emulsion has very good stability at 0.1%, 1% salinity, moderate and high temperature (25 °C and 90 °C) and neutral and alkaline pH (7, 10) that is suitable for the oil reservoirs conditions. The effect of the related nano fluid on the wettability of carbonate rock was investigated by measuring the contact angle and interfacial tension. Results show that the nanofluid could significantly change the wettability of the carbonate rock from oil wet to water wet and can decrease the interfacial tension. Therefore, the 70% MWCNT/SiO{sub 2} nanohybrid Pickering emulsion can be used for Chemical Enhanced Oil Recovery (C-EOR).« less

An inorganic CO2 diffusion and dissolution process explains negative CO2 fluxes in saline/alkaline soils.

PubMed

Ma, Jie; Wang, Zhong-Yuan; Stevenson, Bryan A; Zheng, Xin-Jun; Li, Yan

2013-01-01

An 'anomalous' negative flux, in which carbon dioxide (CO2) enters rather than is released from the ground, was studied in a saline/alkaline soil. Soil sterilization disclosed an inorganic process of CO2 dissolution into (during the night) and out of (during the day) the soil solution, driven by variation in soil temperature. Experimental and modeling analysis revealed that pH and soil moisture were the most important determinants of the magnitude of this inorganic CO2 flux. In the extreme cases of air-dried saline/alkaline soils, this inorganic process was predominant. While the diurnal flux measured was zero sum, leaching of the dissolved inorganic carbon in the soil solution could potentially effect net carbon ecosystem exchange. This finding implies that an inorganic module should be incorporated when dealing with the CO2 flux of saline/alkaline land. Neglecting this inorganic flux may induce erroneous or misleading conclusions in interpreting CO2 fluxes of these ecosystems.

Isolation of a halophilic bacterium, Bacillus sp. strain NY-6 for organic contaminants removal in saline wastewater on ship

NASA Astrophysics Data System (ADS)

Gao, Jie; Yu, Zhenjiang; Zhang, Xiaohui; Zhao, Dan; Zhao, Fangbo

2013-06-01

The objective of this research was to examine if certain strains of Bacillus bacteria, could survive in dry powder products and if so, could the bacteria degrade organic contaminants in saline wastewater on a ship. As part of the study, we isolated 7 domesticated strains named NY1, NY2,..., and NY7, the strain NY6 showed to have the best performance for organic matter degradation and could survive in dry powder more than 3 months. NY6 was identified as Bacillus aerius, based on the morphological and physic-chemical properties. Its optimal growth conditions were as follows: salinity was 2%; temperature was 37°C; pH was in 6.5-7.0; best ratio of C: N: P was 100:5:1. The capability of its dry powder for Chemical Oxygen Demand (COD) removal was 800mg COD/g in synthesized marine wastewater with 2% salinity. The spores in the dry powder were 1.972×108 g -1.

An inorganic CO2 diffusion and dissolution process explains negative CO2 fluxes in saline/alkaline soils

PubMed Central

Ma, Jie; Wang, Zhong-Yuan; Stevenson, Bryan A.; Zheng, Xin-Jun; Li, Yan

2013-01-01

An ‘anomalous' negative flux, in which carbon dioxide (CO2) enters rather than is released from the ground, was studied in a saline/alkaline soil. Soil sterilization disclosed an inorganic process of CO2 dissolution into (during the night) and out of (during the day) the soil solution, driven by variation in soil temperature. Experimental and modeling analysis revealed that pH and soil moisture were the most important determinants of the magnitude of this inorganic CO2 flux. In the extreme cases of air-dried saline/alkaline soils, this inorganic process was predominant. While the diurnal flux measured was zero sum, leaching of the dissolved inorganic carbon in the soil solution could potentially effect net carbon ecosystem exchange. This finding implies that an inorganic module should be incorporated when dealing with the CO2 flux of saline/alkaline land. Neglecting this inorganic flux may induce erroneous or misleading conclusions in interpreting CO2 fluxes of these ecosystems. PMID:23778238

Influence of physico-chemical properties on the abundance of a few economically important juvenile fin-fishes of Vellar estuary.

PubMed

Brinda, S; Bragadeeswaran, S

2005-01-01

Studies on the economically important juvenile fin-fishes such as Elops machnata, Chanos chanos, Lates calcarifer, Epinephelus sp., Sillago sihama, Etroplus suratensis, Mugil cephalus, Liza parsia and Liza tade with relation to the hydrographical parameters as rainfall, temperature, salinity, dissolved oxygen and pH of Vellar estuary during September 2001 to August 2002. The simple correlation co-efficient showed positive significance against juvenile density with water temperature and dissolved oxygen. The influence of hydrographical parameters to the fin-fishes and its abundance is discussed.

Optimization studies on production of a salt-tolerant protease from Pseudomonas aeruginosa strain BC1 and its application on tannery saline wastewater treatment

PubMed Central

Sivaprakasam, Senthilkumar; Dhandapani, Balaji; Mahadevan, Surianarayanan

2011-01-01

Treatment and safe disposal of tannery saline wastewater, a primary effluent stream that is generated by soaking salt-laden hides and skin is one of the major problems faced by the leather manufacturing industries. Conventional treatment methods like solar evaporation ponds and land composting are not eco-friendly as they deteriorate the ground water quality. Though, this waste stream is comprised of high concentration of dissolved proteins the presence of high salinity (1–6 % NaCl by wt) makes it non-biodegradable. Enzymatic treatment is one of the positive alternatives for management of such kind of waste streams. A novel salt-tolerant alkaline protease obtained from P.aeruginosa (isolated from tannery saline wastewater) was used for enzymatic degradation studies. The effect of various physical factors including pH, temperature, incubation time, protein source and salinity on the activity of identified protease were investigated. Kinetic parameters (Km , Vmax) were calculated for the identified alkaline protease at varying substrate concentrations. Tannery saline wastewater treated with identified salt tolerant protease showed 75 % protein removal at 6 h duration and 2 % (v/v) protease addition was found to be the optimum dosage value. PMID:24031785

Surface pH changes suggest a role for H+/OH- channels in salinity response of Chara australis.

PubMed

Absolonova, Marketa; Beilby, Mary J; Sommer, Aniela; Hoepflinger, Marion C; Foissner, Ilse

2018-05-01

To understand salt stress, the full impact of salinity on plant cell physiology has to be resolved. Electrical measurements suggest that salinity inhibits the proton pump and opens putative H + /OH - channels all over the cell surface of salt sensitive Chara australis (Beilby and Al Khazaaly 2009; Al Khazaaly and Beilby 2012). The channels open transiently at first, causing a characteristic noise in membrane potential difference (PD), and after longer exposure remain open with a typical current-voltage (I/V) profile, both abolished by the addition of 1 mM ZnCl 2 , the main known blocker of animal H + channels. The cells were imaged with confocal microscopy, using fluorescein isothiocyanate (FITC) coupled to dextran 70 to illuminate the pH changes outside the cell wall in artificial fresh water (AFW) and in saline medium. In the early saline exposure, we observed alkaline patches (bright fluorescent spots) appearing transiently in random spatial distribution. After longer exposure, some of the spots became fixed in space. Saline also abolished or diminished the pH banding pattern observed in the untreated control cells. ZnCl 2 suppressed the alkaline spot formation in saline and the pH banding pattern in AFW. The osmotic component of the saline stress did not produce transient bright spots or affect banding. The displacement of H + from the cell wall charges, the H + /OH - channel conductance/density, and self-organization are discussed. No homologies to animal H + channels were found. Salinity activation of the H + /OH - channels might contribute to saline response in roots of land plants and leaves of aquatic angiosperms.

Physical and biological forcing of mesoscale variability in the carbonate system of the Ross Sea (Antarctica) during summer 2014

NASA Astrophysics Data System (ADS)

Rivaro, Paola; Ianni, Carmela; Langone, Leonardo; Ori, Carlo; Aulicino, Giuseppe; Cotroneo, Yuri; Saggiomo, Maria; Mangoni, Olga

2017-02-01

Water samples (0-200 m) were collected in a coastal area of the Ross Sea in January 2014 to evaluate the physical and biological forcing on the carbonate system at the mesoscale (distance between stations of 5-10 km). Remote sensing supported the determination of the sampling strategy and helped positioning each sampling station. Total alkalinity, pH, dissolved oxygen, phytoplankton pigments and composition were investigated in combination with measurements of temperature, salinity and current speed. Total inorganic carbon, sea water CO2 partial pressure and the saturation state (Ω) for calcite and aragonite were calculated from the measured total alkalinity and pH. In addition, continuous measurements of atmospheric CO2 concentration were completed. LADCP measurements revealed the presence of a significant change in current speed and direction that corresponded to a clearly defined front characterized by gradients in both temperature and salinity. Phytoplankton biomass was relatively high at all stations and the highest values of chlorophyll-a were found between 20 to 50 m, with the dominant taxonomic group being haptophyceae. The carbonate system properties in surface waters exhibited mesoscale variability with a horizontal length scale of about 10 km. Sea-ice melt, through the input of low salinity water, results in a dilution of the total alkalinity and inorganic carbon, but our observations suggest that phytoplankton activity was the major forcing of the distribution of the carbonate system variables. Higher CO3-, Ω and pH in the surface layer were found where the highest values of chlorophyll-a were observed. The calculated ΔpCO2 pattern follows both MODIS data and in situ chlorophyll-a measurements, and the estimated CO2 fluxes ranged from -0.5 ± 0.4 to -31.0 ± 6.4 mmol m- 2 d- 1. The large range observed in the fluxes is due to both the spatial variability of sea water pCO2 and to the episodic winds experienced.

Remote sensing and avian influenza: A review of image processing methods for extracting key variables affecting avian influenza virus survival in water from Earth Observation satellites

NASA Astrophysics Data System (ADS)

Tran, Annelise; Goutard, Flavie; Chamaillé, Lise; Baghdadi, Nicolas; Lo Seen, Danny

2010-02-01

Recent studies have highlighted the potential role of water in the transmission of avian influenza (AI) viruses and the existence of often interacting variables that determine the survival rate of these viruses in water; the two main variables are temperature and salinity. Remote sensing has been used to map and monitor water bodies for several decades. In this paper, we review satellite image analysis methods used for water detection and characterization, focusing on the main variables that influence AI virus survival in water. Optical and radar imagery are useful for detecting water bodies at different spatial and temporal scales. Methods to monitor the temperature of large water surfaces are also available. Current methods for estimating other relevant water variables such as salinity, pH, turbidity and water depth are not presently considered to be effective.

Environmental factors controlling benthic foraminiferal distribution in Hurghada area, Red Sea coast, Egypt

NASA Astrophysics Data System (ADS)

Mounir El-Kahawy, Ramadan; El-Wahab, Mohamed Abd

2017-04-01

Benthic foraminiferal assemblages were investigated at Hurghada on the Red Sea coast of Egypt, to determine the distribution and their common environmental factors that control on this distribution. 43 sediment samples were collected and environmental factors (T°C, pH, and salinity ‰), water depth, grain size, organic matter and carbonate content were measured. Faunal abundance (14-1755 tests/g) with an average 709 tests/g, and faunal diversity (6-39 specimens) with an average 31 specimen. Cluster analysis was divided the Hurghada site into four distinct biotopes based on the faunal data: Biotope (1) is dominated by a Quinqueloculina seminula& Quinqueloculina laevigata, and Triloculina terquemiana assemblage. Biotope (2) is dominated by a Sorites marginalis & Triloculina trigonula assemblage. Biotope (3) is dominated by an Amphistegina lessonii, Ammonia beccarii and Elphidium spp assemblage. Biotope (4) is dominated by a Peneroplis planatus& Coscinospira hemprichii& Sorites orbiculus and Neorotalia calcar assemblage. Some of the recorded foraminiferal tests showed abnormalities in their apertures, coiling and shape of chambers. The distribution of benthic foraminiferal species is governed by environmental factors such as salinity, temperature, substrates-type, water depth and pH. P. planatus and C. hemprichii positively correlate with extreme salinity and temperature, indicating that these species reflect a warm, arid climate conditions. Aside, the heavy metals (Cu, Cd, Zn, Pb, Ni and Mn) concentrations in the sediment samples were analyzed using ICP-OES. The comparative study between the faunal content and the heavy metals enrichments in each sample displayed positive character indicating the worsening of the environmental conditions. Keywords: benthic foraminifera, Hurghada, Red Sea, Egypt

Technical note: Preservation of Trichomonas vaginalis viability in urine for laboratorial diagnosis by the wet mount examination.

PubMed

da Silva Dias, Mariana; Menezes, Camila Braz; Tasca, Tiana

2016-11-01

This study compared preservative solutions at different temperatures aiming to improve the wet mount for trichomoniasis diagnosis. The glucose-saline pH6.0 solution preserved the trophozoites up to 6h. The urine samples preservation is crucial for diagnosis and we suggest this solution as part of the clinical laboratorial routine. Copyright © 2016 Elsevier B.V. All rights reserved.

Defense Coastal/Estuarine Research Program (DCERP1)

DTIC Science & Technology

2013-05-10

also showed that the greater the mixing height and ventilation rate, the lower the PM2.5 concentrations, which points to the diluting effect of these... ventilation rate, the lower the resulting PM2.5 concentrations, which points to the diluting effects of these parameters on ambient PM2.5 concentration...and discharge (New River, tributary creeks) Chemistry: Nutrients, salinity, pH, oxygen , temperature (New River, NRE, tributary creeks) Sedimentology

Tsukamoto fuzzy implementation to identify the pond water quality of koi

NASA Astrophysics Data System (ADS)

Qur'ania, A.; Verananda, D. I.

2017-01-01

The colour quality of koi was affected by the water quality in the pond. Koi fish have a diversity of types differentiated based on the body colour groups, such as one colour pattern, two colour patterns, three colours patterns and even more. Each colour characteristic of the koi have different handling, particularly in the handling of water quality, this is because the colour pigments in the body was affected by the composition of water quality include temperature, pH, TDS, do and salinity. The data of koi fish used were sanke, sowa, kohaku, shiro, yamabuki, ogon and chagoi. The aim of this study is to make an application to inform the condition of the pool water quality that can help breeders to know the water quality that will improve the handling strategies through water media. Tsukamoto Fuzzy method used to produce the three outputs namely water quality, water grade, and water conditions. The output of water quality consists of four categories, namely optimal, moderate, poor, and very poor. The output of water grade consists of grade A to D, while the output of water conditions consist of an excellent, good, bad, and very bad. Input to the application consists of five parameters, namely water temperature, pH, TDS, do and salinity.

Optimization of Crude Oil and PAHs Degradation by Stenotrophomonas rhizophila KX082814 Strain through Response Surface Methodology Using Box-Behnken Design

PubMed Central

Virupakshappa, Praveen Kumar Siddalingappa; Mishra, Gaurav; Mehkri, Mohammed Ameenuddin

2016-01-01

The present paper describes the process optimization study for crude oil degradation which is a continuation of our earlier work on hydrocarbon degradation study of the isolate Stenotrophomonas rhizophila (PM-1) with GenBank accession number KX082814. Response Surface Methodology with Box-Behnken Design was used to optimize the process wherein temperature, pH, salinity, and inoculum size (at three levels) were used as independent variables and Total Petroleum Hydrocarbon, Biological Oxygen Demand, and Chemical Oxygen Demand of crude oil and PAHs as dependent variables (response). The statistical analysis, via ANOVA, showed coefficient of determination R 2 as 0.7678 with statistically significant P value 0.0163 fitting in second-order quadratic regression model for crude oil removal. The predicted optimum parameters, namely, temperature, pH, salinity, and inoculum size, were found to be 32.5°C, 9, 12.5, and 12.5 mL, respectively. At this optimum condition, the observed and predicted PAHs and crude oil removal were found to be 71.82% and 79.53% in validation experiments, respectively. The % TPH results correlate with GC/MS studies, BOD, COD, and TPC. The validation of numerical optimization was done through GC/MS studies and % removal of crude oil. PMID:28116165

[Survival capacity of Corynebacterium pseudotuberculosis biovar ovis in different soil types from Chubut, Argentine Patagonia].

PubMed

Alvarez, Laura; William, Aillin; Castro, Isabel; Valenzuela, Fernanda; Estevao Belchior, Silvia

Corynebacterium pseudotuberculosis is transmitted among sheep in Argentine Patagonia causing pseudotuberculosis. The bacterium penetrates the skin or mucous membrane wounds, infecting the superficial lymph nodes and viscera. When surface abscesses are cut during shearing, they drain their purulent contents and contaminate tools and the soil. The objective of this work was to evaluate the survival capacity of C. pseudotuberculosis over time, in soils from the extra-Andean Patagonia region. Five types of superficial soils were collected from different areas in Chubut province (extra-Andean Patagonia), having distinctive physicochemical properties including organic matter content (very high to nonexistent), pH (neutral to strongly alkaline), electrical conductivity (saline to non-saline) and texture (sandy, clayey, silty loam). Different aliquots of each type of soil were inoculated with C. pseudotuberculosis PAT10 strain isolated from a Patagonian sheep, and were stored at room temperature. The number of surviving bacteria was determined at various times. Sixty percent (60%) of the inoculated C. pseudotuberculosis population survived for 80 to 210 days in soils with moderate to high organic matter content respectively. Silty soils favored bacterial survival, whereas the variables pH and salinity had no effect on survival. Copyright © 2016 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

[Biodegradation of nitrobenzene by a halophilic Myroides odoratimimus strain Y6].

PubMed

Li, Tian; Qian, Kun; Xiao, Wei; Wang, Jin-Jun; Deng, Xin-Ping

2013-02-01

Aimed at efficient remediation of nitrobenzene-contaminated saline wastewater, the nitrobenzene-degrading characteristics of a Myroides odoratimimus strain Y6 were studied and analyzed. The effects of temperature, pH, initial concentration of nitrobenzene, inoculum concentration and culture type on the biodegradation of nitrobenzene by strain Y6 under saline conditions were studied. Strain Y6 showed the highest efficiency of nitrobenzene degradation in 7% NaC1 (mass fraction). The optimal conditions for the biodegradation of nitrobenzene by strain Y6 were at pH 6.0, 28 degrees C and D600 = 1. With initial concentrations of 100 and 200 mg.L-1, 97.5% and 65.7% of nitrobenzene were transformed after 168 h in 7% NaCL, respectively. Three kinds of additional compounds, glucose, starch and glycerin were tested to choose a favorite carbon source for the co-metabolism of strain Y6. The results showed that all these three compounds could promote nitrobenzene biodegradation and cell growth. And the rate of degradation could increase to 93.3% ,with 800 mg.L-1 glucose as the primary substrate. These results suggest that strain Y6 could be a potential candidate for treating nitrobenzene-contaminated saline wastewater. To date, this is the only study on the degradation of nitrobenzene by M. odoratimimus.

Oil/water/rock wettability: Influencing factors and implications for low salinity water flooding in carbonate reservoirs

DOE PAGES

Chen, Yongqiang; Xie, Quan; Sari, Ahmad; ...

2017-11-21

Wettability of the oil/brine/rock system is an essential petro-physical parameter which governs subsurface multiphase flow behaviour and the distribution of fluids, thus directly affecting oil recovery. Recent studies [1–3] show that manipulation of injected brine composition can enhance oil recovery by shifting wettability from oil-wet to water-wet. However, what factor(s) control system wettability has not been completely elucidated due to incomplete understanding of the geochemical system. To isolate and identify the key factors at play we used in this paper SO 4 2—free solutions to examine the effect of salinity (formation brine/FB, 10 times diluted formation brine/10 dFB, and 100more » times diluted formation brine/100 dFB) on the contact angle of oil droplets at the surface of calcite. We then compared contact angle results with predictions of surface complexation by low salinity water using PHREEQC software. We demonstrate that the conventional dilution approach likely triggers an oil-wet system at low pH, which may explain why the low salinity water EOR-effect is not always observed by injecting low salinity water in carbonated reservoirs. pH plays a fundamental role in the surface chemistry of oil/brine interfaces, and wettability. Our contact angle results show that formation brine triggered a strong water-wet system (35°) at pH 2.55, yet 100 times diluted formation brine led to a strongly oil-wet system (contact angle = 175°) at pH 5.68. Surface complexation modelling correctly predicted the wettability trend with salinity; the bond product sum ([>CaOH 2 +][–COO -] + [>CO 3 -][–NH +] + [>CO 3 -][–COOCa +]) increased with decreasing salinity. Finally, at pH < 6 dilution likely makes the calcite surface oil-wet, particularly for crude oils with high base number. Yet, dilution probably causes water wetness at pH > 7 for crude oils with high acid number.« less

Oil/water/rock wettability: Influencing factors and implications for low salinity water flooding in carbonate reservoirs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Yongqiang; Xie, Quan; Sari, Ahmad

Wettability of the oil/brine/rock system is an essential petro-physical parameter which governs subsurface multiphase flow behaviour and the distribution of fluids, thus directly affecting oil recovery. Recent studies [1–3] show that manipulation of injected brine composition can enhance oil recovery by shifting wettability from oil-wet to water-wet. However, what factor(s) control system wettability has not been completely elucidated due to incomplete understanding of the geochemical system. To isolate and identify the key factors at play we used in this paper SO 4 2—free solutions to examine the effect of salinity (formation brine/FB, 10 times diluted formation brine/10 dFB, and 100more » times diluted formation brine/100 dFB) on the contact angle of oil droplets at the surface of calcite. We then compared contact angle results with predictions of surface complexation by low salinity water using PHREEQC software. We demonstrate that the conventional dilution approach likely triggers an oil-wet system at low pH, which may explain why the low salinity water EOR-effect is not always observed by injecting low salinity water in carbonated reservoirs. pH plays a fundamental role in the surface chemistry of oil/brine interfaces, and wettability. Our contact angle results show that formation brine triggered a strong water-wet system (35°) at pH 2.55, yet 100 times diluted formation brine led to a strongly oil-wet system (contact angle = 175°) at pH 5.68. Surface complexation modelling correctly predicted the wettability trend with salinity; the bond product sum ([>CaOH 2 +][–COO -] + [>CO 3 -][–NH +] + [>CO 3 -][–COOCa +]) increased with decreasing salinity. Finally, at pH < 6 dilution likely makes the calcite surface oil-wet, particularly for crude oils with high base number. Yet, dilution probably causes water wetness at pH > 7 for crude oils with high acid number.« less

Tide- and rainfall-induced variations of physical and chemical parameters in a mangrove-depleted estuary of East Hainan (South China Sea).

PubMed

Krumme, Uwe; Herbeck, Lucia S; Wang, Tianci

2012-12-01

The estuarine dynamics favoring the coexistence of mangroves, seagrass and corals at small river mouths are often poorly understood. We characterize the tidal, day/night and rainfall-induced short-term dynamics in salinity, pH, dissolved oxygen (DO), chlorophyll a (chl a), total suspended matter (TSM), water transparency, surface currents and dissolved nutrients (NO(x)(-), NH(4)(+), PO(4)(3)(-), Si(OH)(4)) of the Wenchang/Wenjiao Estuary (East Hainan, tropical China). Samples were taken at three fixed sites along the estuary during 24 h spring tide cycles in different seasons. Salinity, DO, water transparency and pH generally increased seawards while nutrients decreased. All parameters varied with the tidal cycle, partially in interaction with the diel cycle. Nutrients, chl a and TSM usually fluctuated inversely with water level. Stratification was strong. Inflowing bottom water was of higher salinity, DO and pH and lower temperature and nutrient concentrations than the surface water. Tidal mixing provided regular ventilation of the estuary and limited eutrophication effects of nutrients from aquaculture, agriculture and urban effluents. Under dry weather conditions, the brackish-water lagoon functioned as a sink of nutrients due to efficient uptake by phytoplankton. Presently, the runoff from common intense rains in the watershed affects the estuary with little time delay due to terrestrial deforestation, channelization and loss of mangrove area. The frequency, strength and duration of intermittent estuarization of the back-reef areas have likely increased in the past and deteriorate present seagrass and coral health. Copyright © 2012 Elsevier Ltd. All rights reserved.

The Thermodynamics of the Carbonate System in Seawater,

DTIC Science & Technology

1979-03-08

ionization of water at various water by potentiometric titration . Deep-Sea Res. 17, temperatures from molal volume data. J. Soln. Chem. 737-750. 1... titration alkalinity, AT, givcn by not available at low salinities and molal volume cal- A, = [HCO/] + 2[CO2-] + [B(OH-] culations (MILLERO et al...used to characterize obtained by a computer titration improves, pH the parameters of the carbonate system in seawater. measurements should be made. This

Response of planktonic cladocerans (Class: Branchiopoda) to short-term changes in environmental variables in the surface waters of the Bay of Biscay

NASA Astrophysics Data System (ADS)

d'Elbée, Jean; Lalanne, Yann; Castège, Iker; Bru, Noelle; D'Amico, Frank

2014-08-01

From January 2001 to December 2008, 73 surface plankton samples and 45 vertical profiles of sea temperature, salinity, dissolved oxygen and pH were collected on a monthly basis from a single sampling station located in the Bay of Biscay (43°37N; 1°43W) (North-East Atlantic). Two types of North Atlantic Oscillation (NAO) indexes were included in the data set and submitted to a Canonical Correspondence Analysis and Spearman non-parametric test. Significant breaks and levels in time series were tested using a data segmentation method. The temperature range varies from 11 °C to 25 °C. It begins to rise from April until August and then decline. Low salinity values occur in mid-spring (<34 PSU) and high values (>36 PSU) in autumn. Dissolved oxygen mean values were around 8 mg/l. In summer, when temperature and salinity are high, surface water layer is always accompanied with a significant deoxygenation, and the process reverses in winter. pH mean values range was 7.78-8.33. Seasonal and inter-annual variations of the two NAO indexes are strongly correlated to one another, but do not correlate with any hydrological or biological variable. Five of the seven cladocerans species which are present in the Bay of Biscay were found in this study. There is a strong pattern in species succession throughout the year: Evadne nordmanni is a vernal species, while Penilia avirostris and Pseudevadne tergestina occur mainly in summer and autumn. Evadne spinifera has a maximum abundance in spring, Podon intermedius in autumn, but they both occur throughout the year. However, for some thirty years, the presence of species has tended to become significantly extended throughout the year. During the 2001-2008 period, there was a noticeable decline and even a disappearance of the categories involved in sexual reproduction as well as those involved in parthenogenesis, in favor of non-breeding individuals.

Search for the algorithm of genes distribution during the process of microbial evolution

NASA Astrophysics Data System (ADS)

Pikuta, Elena V.

2015-09-01

Previous two and three dimensional graph analysis of eco-physiological data of Archaea demonstrated specific geometry for distribution of major Prokaryotic groups in a hyperboloid function. The function of a two-sheet hyperboloid covered all known biological groups, and therefore, could be applied for the entire evolution of life on Earth. The vector of evolution was indicated from the point of hyper temperature, extreme acidity and low salinity to the point of low temperature and increased alkalinity and salinity. According to this vector, the following groups were chosen for the gene screening analysis. In the vector "High-Temperature → Low-Temperature" within extreme acidic pH (0-3), it is: 1) the hyperthermophilic Crenarchaeota - order Sulfolobales, 2) moderately thermophilic Euryarchaeota - Class Thermoplasmata, and 3) mesophilic acidophiles- genus Thiobacillus and others. In the vector "Low pH → High pH" the following groups were selected in three temperature ranges: a) Hyperthermophilic Archaea and Eubacteria, b) moderately thermophilic - representatives of the genera Anaerobacter and Anoxybacillus, and c) mesophilic haloalkaliphiles (Eubacteria and Archaea). The genes associated with acidophily (H+ pump), chemolitho-autotrophy (proteins of biochemichal cycles), polymerases, and histones were proposed for the first vector, and for the second vector the genes associated with halo-alkaliphily (Na+ pumps), enzymes of organotrophic metabolisms (sugar- and proteolytics), and others were indicated for the screening. Here, an introduction to the phylogenetic constant (ρη) is presented and discussed. This universal characteristic is calculated for two principally different life forms -Prokaryotes and Eukaryotes; Existence of the second type of living forms is impossible without the first one. The number of chromosomes in Prokaryotic organisms is limited to one (with very rare exceptions, to two), while in Eukaryotic organisms this number is larger. Currently, accumulation of data for genome sequences is in progress: about 3,500 draft sequences of genomes are available (of the total 12,000 species Bacteria and Archaea). The possibility of confirmation of the previously proposed mathematical model with an approach for genes screening in determined key groups of microorganisms in genomes databases is outlined and discussed in this article.

Laboratory investigations of stable carbon and oxygen isotope ratio data enhance monitoring of CO2 underground

NASA Astrophysics Data System (ADS)

Barth, Johannes A. C.; Myrttinen, Anssi; Becker, Veith; Nowak, Martin; Mayer, Bernhard

2014-05-01

Stable carbon and oxygen isotope data play an important role in monitoring CO2 in the subsurface, for instance during carbon capture and storage (CCS). This includes monitoring of supercritical and gaseous CO2 movement and reactions under reservoir conditions and detection of potential CO2 leakage scenarios. However, in many cases isotope data from field campaigns are either limited due to complex sample retrieval or require verification under controlled boundary conditions. Moreover, experimentally verified isotope fractionation factors are also accurately known only for temperatures and pressures lower than commonly found in CO2 reservoirs (Myrttinen et al., 2012). For this reason, several experimental series were conducted in order to investigate effects of elevated pressures, temperatures and salinities on stable carbon and oxygen isotope changes of CO2 and water. These tests were conducted with a heateable pressure device and with glass or metal gas containers in which CO2 reacted with fluids for time periods of hours to several weeks. The obtained results revealed systematic differences in 13C/12C-distributions between CO2 and the most important dissolved inorganic carbon (DIC) species under reservoir conditions (CO2(aq), H2CO3 and HCO3-). Since direct measurements of the pH, even immediately after sampling, were unreliable due to rapid CO2 de-gassing, one of the key results of this work is that carbon isotope fractionation data between DIC and CO2 may serve to reconstruct in situ pH values. pH values reconstructed with this approach ranged between 5.5 and 7.4 for experiments with 60 bars and up to 120 °C and were on average 1.4 pH units lower than those measured with standard pH electrodes directly after sampling. In addition, pressure and temperature experiments with H2O and CO2 revealed that differences between the oxygen isotope ratios of both phases depended on temperature, water-gas ratios as well as salt contents of the solutions involved. Such systematic knowledge of the extent of oxygen isotope fractionation between H2O and CO2 can help to reconstruct equilibration times, fluid-CO2 ratios as well as temperature and salinity conditions. Isotope results from systematic laboratory studies and the information they provide for assessing in situ reservoir conditions can be transferred to field applications concerning integrity of CO2 reservoirs. They can also apply to natural systems and other industrial uses that involve monitoring of gases in the subsurface under similar pressure and temperature conditions. Reference: Myrttinen, A., Becker, V., Barth, J.A.C., 2012. A review of methods used for equilibrium isotope fractionation investigations between dissolved inorganic carbon and CO2. Earth-Science Reviews, 115(3): 192-199.

Beyond temperature: Clumped isotope signatures in dissolved inorganic carbon species and the influence of solution chemistry on carbonate mineral composition

NASA Astrophysics Data System (ADS)

Tripati, Aradhna K.; Hill, Pamela S.; Eagle, Robert A.; Mosenfelder, Jed L.; Tang, Jianwu; Schauble, Edwin A.; Eiler, John M.; Zeebe, Richard E.; Uchikawa, Joji; Coplen, Tyler B.; Ries, Justin B.; Henry, Drew

2015-10-01

;Clumped-isotope; thermometry is an emerging tool to probe the temperature history of surface and subsurface environments based on measurements of the proportion of 13C and 18O isotopes bound to each other within carbonate minerals in 13C18O16O22- groups (heavy isotope ;clumps;). Although most clumped isotope geothermometry implicitly presumes carbonate crystals have attained lattice equilibrium (i.e., thermodynamic equilibrium for a mineral, which is independent of solution chemistry), several factors other than temperature, including dissolved inorganic carbon (DIC) speciation may influence mineral isotopic signatures. Therefore we used a combination of approaches to understand the potential influence of different variables on the clumped isotope (and oxygen isotope) composition of minerals. We conducted witherite precipitation experiments at a single temperature and at varied pH to empirically determine 13C-18O bond ordering (Δ47) and δ18O of CO32- and HCO3- molecules at a 25 °C equilibrium. Ab initio cluster models based on density functional theory were used to predict equilibrium 13C-18O bond abundances and δ18O of different DIC species and minerals as a function of temperature. Experiments and theory indicate Δ47 and δ18O compositions of CO32- and HCO3- ions are significantly different from each other. Experiments constrain the Δ47-δ18O slope for a pH effect (0.011 ± 0.001; 12 ⩾ pH ⩾ 7). Rapidly-growing temperate corals exhibit disequilibrium mineral isotopic signatures with a Δ47-δ18O slope of 0.011 ± 0.003, consistent with a pH effect. Our theoretical calculations for carbonate minerals indicate equilibrium lattice calcite values for Δ47 and δ18O are intermediate between HCO3- and CO32-. We analyzed synthetic calcites grown at temperatures ranging from 0.5 to 50 °C with and without the enzyme carbonic anhydrase present. This enzyme catalyzes oxygen isotopic exchange between DIC species and is present in many natural systems. The two types of experiments yielded statistically indistinguishable results, and these measurements yield a calibration that overlaps with our theoretical predictions for calcite at equilibrium. The slow-growing Devils Hole calcite exhibits Δ47 and δ18O values consistent with lattice equilibrium. Factors influencing DIC speciation (pH, salinity) and the timescale for DIC equilibration, as well as reactions at the mineral-solution interface, have the potential to influence clumped-isotope signatures and the δ18O of carbonate minerals. In fast-growing carbonate minerals, solution chemistry may be an important factor, particularly over extremes of pH and salinity. If a crystal grows too rapidly to reach an internal equilibrium (i.e., achieve the value for the temperature-dependent mineral lattice equilibrium), it may record the clumped-isotope signature of a DIC species (e.g., the temperature-dependent equilibrium of HCO3-) or a mixture of DIC species, and hence record a disequilibrium mineral composition. For extremely slow-growing crystals, and for rapidly-grown samples grown at a pH where HCO3- dominates the DIC pool at equilibrium, effects of solution chemistry are likely to be relatively small or negligible. In summary, growth environment, solution chemistry, surface equilibria, and precipitation rate may all play a role in dictating whether a crystal achieves equilibrium or disequilibrium clumped-isotope signatures.

Differential effects of abiotic factors and host plant traits on diversity and community composition of root-colonizing arbuscular mycorrhizal fungi in a salt-stressed ecosystem.

PubMed

Guo, Xiaohong; Gong, Jun

2014-02-01

Arbuscular mycorrhizal fungi (AMF) were investigated in roots of 18 host plant species in a salinized south coastal plain of Laizhou Bay, China. From 18 clone libraries of 18S rRNA genes, all of the 22 AMF phylotypes were identified into Glomus, of which 18 and 4 were classified in group A and B in the phylogenetic tree, respectively. The phylotypes related to morphologically defined Glomus species occurred generally in soil with higher salinity. AMF phylotype richness, Shannon index, and evenness were not significantly different between root samples from halophytes vs. non-halophytes, invades vs. natives, or annuals vs. perennials. However, AMF diversity estimates frequently differed along the saline gradient or among locations, but not among pH gradients. Moreover, UniFrac tests showed that both plant traits (salt tolerance, life style or origin) and abiotic factors (salinity, pH, or location) significantly affected the community composition of AMF colonizers. Redundancy and variation partitioning analyses revealed that soil salinity and pH, which respectively explained 6.9 and 4.2 % of the variation, were the most influential abiotic variables in shaping the AMF community structure. The presented data indicate that salt tolerance, life style, and origin traits of host species may not significantly affect the AMF diversity in roots, but do influence the community composition in this salinized ecosystem. The findings also highlight the importance of soil salinity and pH in driving the distribution of AMF in plant and soil systems.

Freshwater salinization syndrome on a continental scale.

PubMed

Kaushal, Sujay S; Likens, Gene E; Pace, Michael L; Utz, Ryan M; Haq, Shahan; Gorman, Julia; Grese, Melissa

2018-01-23

Salt pollution and human-accelerated weathering are shifting the chemical composition of major ions in fresh water and increasing salinization and alkalinization across North America. We propose a concept, the freshwater salinization syndrome, which links salinization and alkalinization processes. This syndrome manifests as concurrent trends in specific conductance, pH, alkalinity, and base cations. Although individual trends can vary in strength, changes in salinization and alkalinization have affected 37% and 90%, respectively, of the drainage area of the contiguous United States over the past century. Across 232 United States Geological Survey (USGS) monitoring sites, 66% of stream and river sites showed a statistical increase in pH, which often began decades before acid rain regulations. The syndrome is most prominent in the densely populated eastern and midwestern United States, where salinity and alkalinity have increased most rapidly. The syndrome is caused by salt pollution (e.g., road deicers, irrigation runoff, sewage, potash), accelerated weathering and soil cation exchange, mining and resource extraction, and the presence of easily weathered minerals used in agriculture (lime) and urbanization (concrete). Increasing salts with strong bases and carbonates elevate acid neutralizing capacity and pH, and increasing sodium from salt pollution eventually displaces base cations on soil exchange sites, which further increases pH and alkalinization. Symptoms of the syndrome can include: infrastructure corrosion, contaminant mobilization, and variations in coastal ocean acidification caused by increasingly alkaline river inputs. Unless regulated and managed, the freshwater salinization syndrome can have significant impacts on ecosystem services such as safe drinking water, contaminant retention, and biodiversity. Copyright © 2018 the Author(s). Published by PNAS.

Freshwater salinization syndrome on a continental scale

PubMed Central

Likens, Gene E.; Pace, Michael L.; Utz, Ryan M.; Haq, Shahan; Gorman, Julia; Grese, Melissa

2018-01-01

Salt pollution and human-accelerated weathering are shifting the chemical composition of major ions in fresh water and increasing salinization and alkalinization across North America. We propose a concept, the freshwater salinization syndrome, which links salinization and alkalinization processes. This syndrome manifests as concurrent trends in specific conductance, pH, alkalinity, and base cations. Although individual trends can vary in strength, changes in salinization and alkalinization have affected 37% and 90%, respectively, of the drainage area of the contiguous United States over the past century. Across 232 United States Geological Survey (USGS) monitoring sites, 66% of stream and river sites showed a statistical increase in pH, which often began decades before acid rain regulations. The syndrome is most prominent in the densely populated eastern and midwestern United States, where salinity and alkalinity have increased most rapidly. The syndrome is caused by salt pollution (e.g., road deicers, irrigation runoff, sewage, potash), accelerated weathering and soil cation exchange, mining and resource extraction, and the presence of easily weathered minerals used in agriculture (lime) and urbanization (concrete). Increasing salts with strong bases and carbonates elevate acid neutralizing capacity and pH, and increasing sodium from salt pollution eventually displaces base cations on soil exchange sites, which further increases pH and alkalinization. Symptoms of the syndrome can include: infrastructure corrosion, contaminant mobilization, and variations in coastal ocean acidification caused by increasingly alkaline river inputs. Unless regulated and managed, the freshwater salinization syndrome can have significant impacts on ecosystem services such as safe drinking water, contaminant retention, and biodiversity. PMID:29311318

Water Quality Characteristics of Sembrong Dam Reservoir, Johor, Malaysia

NASA Astrophysics Data System (ADS)

Mohd-Asharuddin, S.; Zayadi, N.; Rasit, W.; Othman, N.

2016-07-01

A study of water quality and heavy metal content in Sembrong Dam water was conducted from April - August 2015. A total of 12 water quality parameters and 6 heavy metals were measured and classified based on the Interim National Water Quality Standard of Malaysia (INWQS). The measured and analyzed parameter variables were divided into three main categories which include physical, chemical and heavy metal contents. Physical and chemical parameter variables were temperature, dissolved oxygen (DO), biochemical oxygen demand (BOD), chemical oxygen demand (COD), total suspended solid (TSS), turbidity, pH, nitrate, phosphate, ammonium, conductivity and salinity. The heavy metals measured were copper (Cu), lead (Pb), aluminium (Al), chromium (Cr), ferum (Fe) and zinc (Zn). According to INWQS, the water salinity, conductivity, BOD, TSS and nitrate level fall under Class I, while the Ph, DO and turbidity lie under Class IIA. Furthermore, values of COD and ammonium were classified under Class III. The result also indicates that the Sembrong Dam water are not polluted with heavy metals since all heavy metal readings recorded were falls far below Class I.

Transient alkalinization of the leaf apoplast stiffens the cell wall during onset of chloride salinity in corn leaves.

PubMed

Geilfus, Christoph-Martin; Tenhaken, Raimund; Carpentier, Sebastien Christian

2017-11-17

During chloride salinity, the pH of the leaf apoplast (pH apo ) transiently alkalizes. There is an ongoing debate about the physiological relevance of these stress-induced pH apo changes. Using proteomic analyses of expanding leaves of corn ( Zea mays L.), we show that this transition in pH apo conveys functionality by (i) adjusting protein abundances and (ii) affecting the rheological properties of the cell wall. pH apo was monitored in planta via microscopy-based ratio imaging, and the leaf-proteomic response to the transient leaf apoplastic alkalinization was analyzed via ultra-high performance liquid chromatography-MS. This analysis identified 1459 proteins, of which 44 exhibited increased abundance specifically through the chloride-induced transient rise in pH apo These elevated protein abundances did not directly arise from high tissue concentrations of Cl - or Na + but were due to changes in the pH apo Most of these proteins functioned in growth-relevant processes and in the synthesis of cell wall-building components such as arabinose. Measurements with a linear-variable differential transducer revealed that the transient alkalinization rigidified ( i.e. stiffened) the cell wall during the onset of chloride salinity. A decrease in t -coumaric and t -ferulic acids indicates that the wall stiffening arises from cross-linkage to cell wall polymers. We conclude that the pH of the apoplast represents a dynamic factor that is mechanistically coupled to cellular responses to chloride stress. By hardening the wall, the increased pH abrogates wall loosening required for cell expansion and growth. We conclude that the transient alkalinization of the leaf apoplast is related to salinity-induced growth reduction. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

[Spatial heterogeneity of soil salinization and its influencing factors in the typical region of the Mu Us Desert-Loess Plateau transitional zone, Northwest China].

PubMed

Zhao, Xuan; Hao, Qi Li; Sun, Ying Ying

2017-06-18

Studies on the spatial heterogeneity of saline soil in the Mu Us Desert-Loess Plateau transition zone are meaningful for understanding the mechanisms of land desertification. Taking the Mu Us Desert-Loess Plateau transition zone as the study subject, its spatial heterogeneity of pH, electrical conductivity (EC) and total salt content were analyzed by using on-site sampling followed with indoor analysis, classical statistical and geostatistical analysis. The results indicated that: 1) The average values of pH, EC and total salt content were 8.44, 5.13 mS·cm -1 and 21.66 g·kg -1 , respectively, and the coefficient of variation ranged from 6.9% to 73.3%. The pH was weakly variable, while EC and total salt content were moderately variable. 2) Results of semivariogram analysis showed that the most fitting model for spatial variability of all three indexes was spherical model. The C 0 /(C 0 +C) ratios of three indexes ranged from 8.6% to 14.3%, which suggested the spatial variability of all indexes had a strong spatial autocorrelation, and the structural factors played a more important role. The variation range decreased in order of pH

The Habitat of Yellow Mouth Turban Turbo Chrysostomus, Linnaeus, 1758

NASA Astrophysics Data System (ADS)

Soekendarsi, E.

2018-03-01

In general, yellow mouth turban snail Turbo chrysostomus L. 1758 was found in intertidal and coral reef area. This animal is active at night (nocturnal) and settles the coral reef-flats area to do its activity as substrate. In doing its activity, yellow mouth turban snail can be found in the depth of 50 cm until 4 m of tidal area. The adult yellow mouth turban snails are found in great number at intertidal area’s border and at coastal area of coral reef-flats. Methodology that was used in this study is visual analysis (descriptive method), and divided into two parameters which were observed, i.e. abiotic and biotic. Abiotic components that were measured are; Oxygen (ppm), pH, Water Temperature (°C), Salinity (ppm), Ammonia (mg/L), Nitrate (mg/L), Nitrite (mg/L), and Calsium Carbonat (mg/L).Whereas, biotic components that were measured are; substrates, seaweeds, other organisms, and epilithon. The observation’s result of yellow mouth turban snail’s environmental condition showed: abiotic condition of the waters consists of oxygen 3-5 ppm, seawater pH 7-8, seawater temperature 23-26°C, and the salinity of 32-33 ppm. The Habitat of yellow mouth turban snail settled the reef-flats area that is overgrown covered by seaweed Sargassum sp. as the place to do its activity.

Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions.

PubMed

Magnotti, C; Figueroa, E; Farias, J G; Merino, O; Valdebenito, I; Oliveira, R P S; Cerqueira, V

2018-05-01

In this article we describe basic aspects of the sperm biology of lebranche mullet (Mugil liza) in the wild and in captivity, in particular assessing the effects of salinity (0, 10, 20, 30, 35, 40, 50 and 60 g L -1 ) and pH (6, 7, 8, 9 and 10) on sperm motility. Our results indicate that the highest percentage of motility was recorded with salinity 34.6 g L -1 (95 ± 10%) and the longest motility time was obtained with a salinity of 34.8 g L -1 (189 ± 15 s). Variations in the salinity between 30 and 35 g L -1 did not produce any significant alterations in sperm motility; however salinities of 20 and 50 g L -1 produced a significant loss of sperm motility. The highest percentage of motility was obtained at pH 8.5 (93 ± 12%), and the longest motility period at pH 8.7 (218 ± 13 s), while pH lower than or equal to 7 and equal to 10 both produced a significant loss in sperm motility. A positive correlation was found between pH/salinity and the motility percentage (R 2  = 0.94 and R 2  = 0.97) and motility time (R 2  = 0.86 and R 2  = 0.98). In seminal and morphometric parameters, statistically significant differences were observed in semen volume, sperm density, plasma membrane integrity and sperm morphometry between the groups studied, showing that the characteristics of the fish have a direct influence on sperm quality. The information generated in this research will be useful for developing biotechnology tools for the effective management of Mugil liza gametes. Copyright © 2018 Elsevier B.V. All rights reserved.

A microstructural study of the degradation and calcium release from hydroxyapatite-calcium oxide ceramics made by infiltration.

PubMed

Zhang, Qinghao; Schmelzer, Eva; Gerlach, Jörg C; Nettleship, Ian

2017-04-01

Hydroxyapatite pellets, partially densified in a low-temperature heat treatment, were infiltrated with calcium nitrate solution followed by in-situ precipitation of Ca(OH) 2 and CaCO 3 . The infiltrated bodies were then densified to high relative density and the calcium carbonate transformed to calcium oxide during sintering and resulted in biphasic hydroxyapatite-CaO ceramics. This work investigated the influence of the infiltration on surface morphology, weight change, and microstructural-level degradation caused by exposure to saline at pH=7.4 and a temperature of 20°C. The CaO rendered the materials more susceptible to degradation, and released calcium into the saline faster than single phase, calcium deficient hydroxyapatite (HA) that were used as a control. In consequence, these ceramics could be used to release calcium into the culture microenvironments of bone tissue or bone marrow cells next to a scaffold surface. Copyright © 2016 Elsevier B.V. All rights reserved.

Influence of temperature, pH, and salinity on membrane lipid composition and TEX86 of marine planktonic thaumarchaeal isolates

NASA Astrophysics Data System (ADS)

Elling, Felix J.; Könneke, Martin; Mußmann, Marc; Greve, Andreas; Hinrichs, Kai-Uwe

2015-12-01

Marine ammonia-oxidizing archaea of the phylum Thaumarchaeota are a cosmopolitan group of microorganisms representing a major fraction of the picoplankton in the ocean. The cytoplasmic membranes of Thaumarchaeota consist predominantly of intact polar isoprenoid glycerol dibiphytanyl glycerol tetraether (GDGT) lipids, which may be used as biomarkers for living Thaumarchaeota. Fossil thaumarchaeal GDGT core lipids accumulate in marine sediments and serve as the basis for geochemical proxies such as the TEX86 paleothermometer. Here, we demonstrate that the responses of membrane lipid compositions and resulting TEX86 values to growth temperature strongly diverge in three closely related thaumarchaeal pure cultures, i.e., Nitrosopumilus maritimus and two novel strains isolated from South Atlantic surface water, although the inventories of intact polar lipids and core lipids were overall similar in the three strains. N. maritimus and its closely related strain NAOA6 showed linear relationships of TEX86 and growth temperature but no correlation of TEX86 and temperature was observed in the more distantly related strain NAOA2. In contrast, the weighted average number of cycloalkyl moieties (ring index) was linearly correlated with growth temperature in all strains. This disparate relationship of TEX86 to growth temperature among closely related Thaumarchaeota suggests that the ring index but not the TEX86 ratio represents a universal response to growth temperature in marine planktonic Thaumarchaeota. Furthermore, the distinct TEX86-temperature relationships in the cultivated strains indicate that environmental GDGT signals may include an ecological component, which has important implications for ocean temperature reconstructions using the TEX86 proxy. In contrast, different growth medium salinities in the range 27-51‰ tested for N. maritimus showed no systematic effect on intact polar GDGT composition and TEX86. Similarly, N. maritimus showed only small changes in intact polar GDGT composition and TEX86 when grown at different medium pH in the range 7.3-7.9. Overall, our pure culture studies suggest that the TEX86 paleotemperature proxy is not solely dependent on growth temperature, but may amalgamate physiological, environmental, and ecological factors.

Salinity and Alkaline pH in Irrigation Water Affect Marigold Plants: II. Mineral Ion Relations

USDA-ARS?s Scientific Manuscript database

Scarcity of water of good quality for landscape irrigation is of outmost importance in arid and semiarid regions due to the competition with urban population. This is forcing the use of degraded waters with high levels of salinity and high pH, which may affect plant establishment and growth. The o...

Salinity/temperature ranges for application of seawater SA-T-P models

NASA Astrophysics Data System (ADS)

Marion, G. M.; Millero, F. J.; Feistel, R.

2009-01-01

At the present time, little is known about how broad salinity and temperature ranges are for seawater thermodynamic models that are functions of absolute salinity (SA), temperature (T) and pressure (P). Such models rely on fixed compositional ratios of the major components (e.g. Na/Cl, Mg/Cl, Ca/Cl, SO4/Cl, etc.). As seawater evaporates or freezes, solid phases (e.g. CaCO3(s) or CaSO42H2O(s)) will eventually precipitate. This will change the compositional ratios, and these salinity models will no longer be applicable. A future complicating factor is the lowering of seawater pH as the atmospheric concentrations of CO2 increase. A geochemical model (FREZCHEM) was used to quantify the SA-T boundaries at P=0.1 MPa and the range of these boundaries for future atmospheric CO2 increases. An omega supersaturation model for CaCO3 minerals based on homogeneous nucleation was extended from 25-40°C to 3°C. CaCO3 minerals were the boundary defining minerals (first to precipitate) between 3°C (at SA=104 g kg-1 and 40°C (at SA=66 g kg-1. At 2.82°C, calcite(CaCO3) transitioned to ikaite(CaCO36H2O) as the dominant boundary defining mineral for colder temperatures, which culminated in a low temperature boundary of -4.93°C. Increasing atmospheric CO2 from 385 μatm (in Year 2008) to 550 μatm (in Year 2100) would increase the SA and t boundaries as much as 11 g kg-1 and 0.66°C, respectively. The model-calculated calcite-ikaite transition temperature of 2.82°C is in excellent agreement with ikaite formation in natural environments that occurs at temperatures of 3°C or lower. Furthermore, these results provide a quantitative theoretical explanation (FREZCHEM model calculations) for why ikaite is the solid phase CaCO3 mineral that precipitates during seawater freezing.

Adsorption of phosphate from seawater on calcined MgMn-layered double hydroxides.

PubMed

Chitrakar, Ramesh; Tezuka, Satoko; Sonoda, Akinari; Sakane, Kohji; Ooi, Kenta; Hirotsu, Takahiro

2005-10-01

Adsorptive properties of MgMn-3-300 (MgMn-type layered double hydroxide with Mg/Mn mole ratio of 3, calcined at 300 degrees C) for phosphate were investigated in phosphate-enriched seawater with a concentration of 0.30 mg-P/dm3. It showed the highest phosphate uptake from the seawater among the inorganic adsorbents studied (hydrotalcite, calcined hydrotalcite, activated magnesia, hydrous aluminum oxide, manganese oxide (delta-MnO2)). The phosphate uptake by MgMn-3-300 reached 7.3 mg-P/g at an adsorbent/solution ratio of 0.05 g/2 dm3. The analyses of the uptakes of other constituents (Na+, K+, Ca(+, Cl-, and SO(2-)4) of seawater showed that the adsorbent had a markedly high selectivity for the adsorption of phosphate ions. Effects of initial phosphate concentration, temperature, pH, and salinity on phosphate uptake were investigated in detail by a batch method. The phosphate uptake increased slightly with an increase in the adsorption temperature. The adsorption isotherm followed Freundlich's equation with constants of logK(F)=1.25 and 1/n=0.65, indicating that it could effectively remove phosphate even from a solution of markedly low phosphate concentration as well as with large numbers of coexisting ions. The pH dependence showed a maximum phosphate uptake around pH 8.5. The pH dependence curve suggested that selective phosphate adsorption progresses mainly by the ion exchange of HPO(2-)4. The study on the effect of salinity suggested the presence of two kinds of adsorption sites in the adsorbent: one nonspecific site with weak interaction and one specific site with strong interaction. The effective desorption of phosphate could be achieved using a mixed solution of 5 M NaCl + 0.1 M NaOH (1 M = 1 mol/dm3), with negligible dissolution of adsorbent. The adsorbent had high chemical stability against the adsorption/desorption cycle; it kept a good phosphate uptake even after the repetition of the seventh cycle.

Stability of solutions of antineoplastic agents during preparation and storage for in vitro assays. General considerations, the nitrosoureas and alkylating agents.

PubMed

Bosanquet, A G

1985-01-01

In vitro drug sensitivity of tumour biopsies is currently being determined using a variety of methods. For these chemosensitivity assays many drugs are required at short notice, and this in turn means that the drugs must generally be stored in solution. There are, however, a number of potential problems associated with dissolving and storing drugs for in vitro use, which include (a) drug adsorption; (b) effects of freezing; (c) drug stability under the normal conditions of dilution and setting up of an in vitro assay; and (d) insolubility of drugs in normal saline (NS) or phosphate-buffered saline (PBS). These problems are considered in general, and some recommendations for use of solutions of drugs in in vitro assays are suggested. The nitrosoureas and alkylating agents are also investigated in greater detail in this respect. The nitrosoureas are found to be very labile in PBS at pH 7, with 5% degradation (t0.95) occurring in 10-50 min at room temperature. These values are increased about 10-fold on refrigeration and about 5- to 10-fold on reduction of the pH of the medium to pH 4-5. At pH 7 and room temperature, t0.95 is observed in under 1 h with the alkylating agents nitrogen mustard, chlorambucil, melphalan, 2,5-diaziridinyl-3,6-bis(2-hydroxyethylamino)-1,4-benzoquinone (BZQ), dibromodulcitol, dibromomannitol, treosulphan, and procarbazine. Of the other alkylating agents, 4-hydroperoxycylophosphamide (sometimes used in vitro in place of cyclophosphamide), busulphan, dianhydrogalactitol, aziridinylbenzoquinone (AZQ), and dacarbazine have a t0.95 of between 2 and 24 h, while ifosfamide and pentamethylmelamine are both stable in aqueous solution for greater than 7 days. About half the drugs studied in detail have been stored frozen in solution for in vitro use, although very little is known about their stability under these conditions.

Salinity and temperature tolerance of brown-marbled grouper Epinephelus fuscoguttatus.

PubMed

Cheng, Sha-Yen; Chen, Chih-Sung; Chen, Jiann-Chu

2013-04-01

Grouper have to face varied environmental stressors as a result of drastic changes to water conditions during the storm season. We aimed to test the response of brown-marbled grouper to drastic and gradual changes in temperature and salinity to understand the grouper's basic stress response. The results can improve the culture of grouper. Brown-marbled grouper, Epinephelus fuscoguttatus (6.2 ± 0.8 g) were examined for temperature and salinity tolerances at nine different environmental regimes (10, 20, and 33 ‰ combined with 20, 26 and 32 °C), in which the fish were subjected to both gradual and sudden changes in temperature and salinity. The critical thermal maximum (50 % CTMAX) and the upper incipient lethal temperature (UILT) were in the ranges of 35.9-38.3 and 32.7-36.5 °C, respectively. The critical thermal minimum (50 % CTMIN) and the lower incipient lethal temperature (LILT) were in the ranges of 9.8-12.2 and 14.9-22.3 °C, respectively. The critical salinity maximum (50 % CSMAX) and the upper incipient lethal salinity (UILS) were in the ranges of 67.0-75.5 and 54.2-64.8 ‰, respectively. Fish at temperature of 20 °C and a salinity of 33 ‰ tolerated temperatures as low as 10 °C when the temperature was gradually decreased. Fish acclimated at salinities of 10-33 ‰ and a temperature of 32 °C tolerated salinities of as high as 75-79 ‰. All fish survived from accumulating salinity after acute transfer to 20, 10, 5, and 3 ‰. But all fish died while transferred to 0 ‰. Relationships among the UILT, LILT, 50 % CTMAX, 50 % CTMIN, UILS, 50 % CSMAX, salinity, and temperature were examined. The grouper's temperature and salinity tolerance elevated by increasing acclimation temperature and salinity. On the contrary, the grouper's temperature and salinity tolerance degraded by decreasing acclimation temperature and salinity. The tolerance of temperature and salinity on grouper in gradual changes were higher than in drastic changes.

Dynamic changes in water and salinity in saline-alkali soils after simulated irrigation and leaching.

PubMed

Wang, Shutao; Feng, Qian; Zhou, Yapeng; Mao, Xiaoxi; Chen, Yaheng; Xu, Hao

2017-01-01

Soil salinization is a global problem that limits agricultural development and impacts human life. This study aimed to understand the dynamic changes in water and salinity in saline-alkali soil based on an indoor soil column simulation. We studied the changes in the water and salt contents of soils with different degrees of salinization under various irrigation conditions. The results showed that after seven irrigations, the pH, conductivity and total soluble salt content of the percolation samples after irrigation generally increased initially then decreased with repeated irrigation. The soil moisture did not change significantly after irrigation. The pH, conductivity, and total soluble salt content of each layer of the soil profile exhibited general declining trends. In the soil profile from Changguo Township (CG), the pH decreased from 8.21-8.35 to 7.71-7.88, the conductivity decreased from 0.95-1.14 ms/cm to 0.45-0.68 ms/cm, and the total soluble salt content decreased from 2.63-2.81 g/kg to 2.28-2.51 g/kg. In the soil profile from Zhongjie Industrial Park (ZJ), the pH decreased from 8.36-8.54 to 7.73-7.96, the conductivity decreased from 1.58-1.68 ms/cm to 1.45-1.54 ms/cm, and the total soluble salt decreased from 2.81-4.03 g/kg to 2.56-3.28 g/kg. The transported salt ions were primarily K+, Na+ and Cl-. After several irrigations, a representative desalination effect was achieved. The results of this study can provide technical guidance for the comprehensive management of saline-alkali soils.

Dynamic changes in water and salinity in saline-alkali soils after simulated irrigation and leaching

PubMed Central

Feng, Qian; Mao, Xiaoxi

2017-01-01

Soil salinization is a global problem that limits agricultural development and impacts human life. This study aimed to understand the dynamic changes in water and salinity in saline-alkali soil based on an indoor soil column simulation. We studied the changes in the water and salt contents of soils with different degrees of salinization under various irrigation conditions. The results showed that after seven irrigations, the pH, conductivity and total soluble salt content of the percolation samples after irrigation generally increased initially then decreased with repeated irrigation. The soil moisture did not change significantly after irrigation. The pH, conductivity, and total soluble salt content of each layer of the soil profile exhibited general declining trends. In the soil profile from Changguo Township (CG), the pH decreased from 8.21–8.35 to 7.71–7.88, the conductivity decreased from 0.95–1.14 ms/cm to 0.45–0.68 ms/cm, and the total soluble salt content decreased from 2.63–2.81 g/kg to 2.28–2.51 g/kg. In the soil profile from Zhongjie Industrial Park (ZJ), the pH decreased from 8.36–8.54 to 7.73–7.96, the conductivity decreased from 1.58–1.68 ms/cm to 1.45–1.54 ms/cm, and the total soluble salt decreased from 2.81–4.03 g/kg to 2.56–3.28 g/kg. The transported salt ions were primarily K+, Na+ and Cl-. After several irrigations, a representative desalination effect was achieved. The results of this study can provide technical guidance for the comprehensive management of saline-alkali soils. PMID:29091963

One hundred years of hydrographic measurements in the Baltic Sea

NASA Astrophysics Data System (ADS)

Fonselius, Stig; Valderrama, Jorge

2003-06-01

The first measurements of salinity of the deep water in the open Baltic Sea were made in the last decades of the 1800s. At a Scandinavian science meeting in Copenhagen in 1892, Professor Otto Pettersson from Sweden suggested that regular measurements of hydrographic parameters should be carried out at some important deep stations in the Baltic Sea. His suggestion was adopted and since that time we have rather complete hydrographical data from the Bornholm Deep, the Gotland Deep, and the Landsort Deep and from some stations in the Gulf of Bothnia. The measurements were interrupted in the Baltic Proper during the two World Wars. At the beginning only salinity, temperature and dissolved oxygen were measured and one or two expeditions were carried out annually, mostly in summer. In the 1920s also alkalinity and pH were occasionally measured and total carbonate was calculated. A few nutrient measurements were also carried out. After World War II we find results from four or more expeditions every year and intercalibration of methods was arranged. Results of temperature, salinity and dissolved oxygen measurements from the Bornholm Deep, the Gotland Deep, the Landsort Deep and salinity measurements from three stations in the Gulf of Bothnia, covering the whole 20th century are presented and discussed. The salinity distribution and the variations between oxygen and hydrogen sulphide periods in the deep water of the Gotland Deep and the Landsort Deep are demonstrated. Series of phosphate and nitrate distribution in the Gotland Deep are shown from the 1950s to the present and the effects of the stagnant conditions are briefly discussed. Two large inflows of highly saline water, the first during the First World War and the second in 1951, are demonstrated. The 20th century minimum salinity of the bottom water in the Baltic Proper in 1992 is discussed.

Distribution and abundance of fish eggs and larvae in Arasalar estuary, Karaikkal, south-east coast of India.

PubMed

Rajasegar, M; Bragadeeswaran, S; Kumar, R Senthil

2005-04-01

The distribution and abundance of fish eggs and larvae have been studied at two Stations of Arasalar estuary. Eggs of Stolephorus sp., Mugil cephalus, Sardinella sp., Cynoglossus sp and the larvae of Stolephorus indicus, Ambassis commersoni, Terapon jarbua, Chanos chanos, Trichiurus sp Anguilla sp and Thryssa sp have been collected and identified. Environmental parameters such as temperature, salinity, pH and dissolved oxygen were also recorded. The present study clearly indicate the higher occurrence and abundant of fish eggs and larvae during March - July. The fair numbers of eggs and larvae indicate the existence of breeding ground within the estuary. Study on the physico-chemical parameters found that the abundance and distribution of fish eggs and larvae were influenced by the salinity of this estuary.

pH and Organic Carbon Dose Rates Control Microbially Driven Bioremediation Efficacy in Alkaline Bauxite Residue.

PubMed

Santini, Talitha C; Malcolm, Laura I; Tyson, Gene W; Warren, Lesley A

2016-10-18

Bioremediation of alkaline tailings, based on fermentative microbial metabolisms, is a novel strategy for achieving rapid pH neutralization and thus improving environmental outcomes associated with mining and refining activities. Laboratory-scale bioreactors containing bauxite residue (an alkaline, saline tailings material generated as a byproduct of alumina refining), to which a diverse microbial inoculum was added, were used in this study to identify key factors (pH, salinity, organic carbon supply) controlling the rates and extent of microbially driven pH neutralization (bioremediation) in alkaline tailings. Initial tailings pH and organic carbon dose rates both significantly affected bioremediation extent and efficiency with lower minimum pHs and higher extents of pH neutralization occurring under low initial pH or high organic carbon conditions. Rates of pH neutralization (up to 0.13 mM H + produced per day with pH decreasing from 9.5 to ≤6.5 in three days) were significantly higher in low initial pH treatments. Representatives of the Bacillaceae and Enterobacteriaceae, which contain many known facultative anaerobes and fermenters, were identified as key contributors to 2,3-butanediol and/or mixed acid fermentation as the major mechanism(s) of pH neutralization. Initial pH and salinity significantly influenced microbial community successional trajectories, and microbial community structure was significantly related to markers of fermentation activity. This study provides the first experimental demonstration of bioremediation in bauxite residue, identifying pH and organic carbon dose rates as key controls on bioremediation efficacy, and will enable future development of bioreactor technologies at full field scale.

Archaea in aquaria: Investigating the role of environmental influences on TEX86 in long-term culture experiments

NASA Astrophysics Data System (ADS)

Warren, C.; Pagani, M.

2016-12-01

The TEX86 paleotemperature proxy has experienced a recent rise in application, due in part to its capacity to estimate temperatures above 30°C, and to the ubiquity of the archaeal membrane lipids that comprise it. Studies comparing environmental influences and archaeal lipids - specifically core glycerol dibiphytanyl glycerol tetraethers (GDGTs) - offer insights into non-temperature influences on TEX86. Here we present environmental measurements and lipid distributions from >200 long-running aquarium systems using source water from North American coastal sites ranging in latitude from 60.12ºN to 24.56ºN. The influence of 32 environmental variables (depth, pH, etc.) was evaluated in the entire dataset, as well as in a subset that limited the influence of community structure. Based on multiple correlation analyses we demonstrate that temperature is the central factor influencing the distribution of core GDGTs in aquarium tanks, with the possible exception of GDGT-1, for which dissolved oxygen concentration is equivocally significant when GDGT-4 is included in the calculation of relative abundances. Temperature was found to have the most significant relationship with TEX86, with neither oxygen concentration nor water density making comparable contributions. Salinity, pH, and community structure have emerged as important, but less significant secondary influences of TEX86. Low salinity (<15 PSU) was consistently associated with TEX86 values that substantially overestimate tank temperature. The presence and abundance of MG-II Euryarchaeota, when considered, do not appear to significantly change the TEX86-temperature relationship. In addition, Illumina MiSeq 16S rDNA sequencing was used to assess whether archaeal communities differed along a spectrum of TEX86 values and environmental conditions. This technique allowed for the assessment of a taxonomically constrained subset of samples (n=54) where the archaeal tank populations were >98% sequences recognized as associated with the genus Nitrosopumilus (cultured ammonia oxidizers). In this dataset the relative abundance of GDGT-0 provided a better estimate of temperature than TEX86, and unusually high nitrite concentrations strongly correlated with underestimates of TEX86-based temperature relative to measured water temperature.

Geochemical Modeling of Zinc Silicate Ore Formation from Sedimentary Hydrothermal Fluids

NASA Astrophysics Data System (ADS)

Appold, M. S.

2008-12-01

Sediment-hosted zinc deposits dominated by willemite (Zn2SiO4) instead of sphalerite (ZnS) are known from several prominent occurrences worldwide, including Vazante, Brazil, the Aroona Trend, Australia, Kabwe, Zambia, Berg Aukas, Namibia, and Abu Samar, Sudan. Although willemite-dominant zinc deposits appear to be much less common and are on average smaller than sphalerite-dominant zinc deposits, they nonetheless represent major enrichments of zinc in the Earth's crust, reaching sizes on the order of 1's to 10's of millions of tons and grades commonly between 20 and 40%. Sediment-hosted willemite- and sphalerite-dominant deposits share many similarities including their predominantly carbonate host rocks, gangue mineralogy, presumed derivation from sedimentary basinal brines, and spatial proximity. However, the conditions and processes that led to one style of mineralization versus the other have only recently begun to be investigated. The current study presents solubility, reaction path, and reactive transport modeling results that attempt to define more clearly the conditions that favor willemite ore formation in sedimentary basins, with a focus on the Vazante deposit. Solubility calculations for willemite and sphalerite as a function of temperature, pH, salinity, and oxidation potential were carried out using a simple 3 molal NaCl solution saturated with respect to quartz. The results show that (1) willemite solubility is relatively insensitive to changes in temperature and oxidation potential whereas sphalerite solubility decreases sharply with decreasing temperature and oxidation potential, (2) willemite solubility decreases more strongly than sphalerite with increasing pH, (3) willemite and sphalerite have a similar strong decrease in solubility with decreasing salinity. The results support a previously proposed genetic model for a willemite-dominant, sphalerite-subordinate ore body like Vazante in which a hot, acidic, metal-rich ore fluid mixed with a cooler, more oxidizing, dilute, and basic fluid. This scenario was investigated further with reaction path and reactive transport modeling. In these models, a more complex ore fluid was used that was assumed to have a major element composition similar to the global average for Mississippi Valley-type (MVT) deposits determined from the literature, modified by heating from 150 to 300° C, saturated with respect to dolomite and quartz, moderately acidic, and an oxidation potential near the value defined by magnetite-hematite equilibrium. The ore fluid was allowed to mix with a second, possibly meteoric fluid with about three orders of magnitude lower salinity, neutral pH, and a temperature of 50° C. The modeling results showed general agreement with the mineral assemblage observed at Vazante, and confirmed the need for a strong pH increase to induce willemite precipitation, and no more than a moderate increase in oxidation potential to allow some sphalerite to precipitate. The localization of mineralization within a shear zone was found to depend strongly on the shear zone having acted as a high permeability conduit for the ore fluid from deeper parts of the sedimentary basin.

Investigation of factors affecting in vitro doxorubicin release from PEGylated liposomal doxorubicin for the development of in vitro release testing conditions.

PubMed

Shibata, Hiroko; Izutsu, Ken-Ichi; Yomota, Chikako; Okuda, Haruhiro; Goda, Yukihiro

2015-01-01

Establishing appropriate drug release testing methods of liposomal products for assuring quality and performance requires the determination of factors affecting in vitro drug release. In this study, we investigated the effects of test conditions (human plasma lot, pH/salt concentration in the test media, dilution factor, temperature, ultrasound irradiation, etc.), and liposomal preparation conditions (pH/concentration of ammonium sulfate solution), on doxorubicin (DXR) release from PEGylated liposomal DXR. Higher temperature and lower pH significantly increased DXR release. The evaluation of DXR solubility indicated that the high DXR release induced by low pH may be attributed to the high solubility of DXR at low pH. Ultrasound irradiation induced rapid DXR release in an amplitude-dependent manner. The salt concentration in the test solution, human plasma lot, and dilution factor had a limited impact on DXR-release. Variations in the ammonium sulfate concentration used in solutions for the formation/hydration of liposomes significantly affected DXR release behavior, whereas differences in pH did not. In addition, heating condition in phosphate-buffered saline at lower pH (<6.5) exhibited higher discriminative ability for the release profiles from various liposomes with different concentrations of ammonium sulfate than did ultrasound irradiation. These results are expected to be helpful in the process of establishing appropriate drug release testing methods for PEGylated liposomal DXR.

Fine-Scale Variability in Temperature, Salinity, and pH in the Upper-Ocean and the Effects on Acoustic Transmission Loss in the Western Arctic Ocean.

DTIC Science & Technology

2010-03-01

28  V.  ANALYTIC METHOD AND DATA ANALYSIS ..................................................29  A.  DATA PROCESSING ...your help getting ready for the Arctic and with the data processing back at NPS. Thank you to Professor John Colosi and LCDR Ben Jones for your help...light. Acoustic energy, however, can propagate for very long ranges (Kinsler et al. 2000). This energy can be passively received and processed , and

The stoichiometric dissociation constants of carbonic acid in seawater brines from 298 to 267 K

NASA Astrophysics Data System (ADS)

Papadimitriou, Stathys; Loucaides, Socratis; Rérolle, Victoire M. C.; Kennedy, Paul; Achterberg, Eric P.; Dickson, Andrew G.; Mowlem, Matthew; Kennedy, Hilary

2018-01-01

The stoichiometric dissociation constants of carbonic acid (K1C∗ and K2C∗) were determined by measurement of all four measurable parameters of the carbonate system (total alkalinity, total dissolved inorganic carbon, pH on the total proton scale, and CO2 fugacity) in natural seawater and seawater-derived brines, with a major ion composition equivalent to that of Reference Seawater, to practical salinity (SP) 100 and from 25 °C to the freezing point of these solutions and -6 °C temperature minimum. These values, reported in the total proton scale, provide the first such determinations at below-zero temperatures and for SP > 50. The temperature (T, in Kelvin) and SP dependence of the current pK1C∗ and pK2C∗ (as negative common logarithms) within the salinity and temperature ranges of this study (33 ≤ SP ≤ 100, -6 °C ≤ t ≤ 25 °C) is described by the following best-fit equations: pK1C∗ = -176.48 + 6.14528 SP0.5 - 0.127714 SP + 7.396 × 10-5SP2 + (9914.37 - 622.886 SP0.5 + 29.714 SP) T-1 + (26.05129 - 0.666812 SP0.5) lnT (σ = 0.011, n = 62), and pK2C∗ = -323.52692 + 27.557655 SP0.5 + 0.154922 SP - 2.48396 × 10-4 SP2 + (14763.287 - 1014.819 SP0.5 - 14.35223 SP) T-1 + (50.385807 - 4.4630415 SP0.5) lnT (σ = 0.020, n = 62). These functions are suitable for application to investigations of the carbonate system of internal sea ice brines with a conservative major ion composition relative to that of Reference Seawater and within the temperature and salinity ranges of this study.

Retrospective analysis of associations between water quality and toxic blooms of golden alga (Prymnesium parvum) in Texas reservoirs: Implications for understanding dispersal mechanisms and impacts of climate change

USGS Publications Warehouse

Patino, Reynaldo; Dawson, D.; VanLandeghem, Matthew M.

2014-01-01

Toxic blooms of golden alga (GA, Prymnesium parvum) in Texas typically occur in winter or early spring. In North America, they were first reported in Texas in the 1980s, and a marked range expansion occurred in 2001. Although there is concern about the influence of climate change on the future distribution of GA, factors responsible for past dispersals remain uncertain. To better understand the factors that influence toxic bloom dispersal in reservoirs, this study characterized reservoir water quality associated with toxic GA blooms since 2001, and examined trends in water quality during a 20-year period bracketing the 2001 expansion. Archived data were analyzed for six impacted and six nonimpacted reservoirs from two major Texas basins: Brazos River and Colorado River. Data were simplified for analysis by pooling spatially (across sampling stations) and temporally (winter, December-February) within reservoirs and generating depth-corrected (1 m) monthly values. Classification tree analysis [period of record (POR), 2001-2010] using salinity-associated variables (specific conductance, chloride, sulfate), dissolved oxygen (DO), pH, temperature, total hardness, potassium, nitrate+nitrite, and total phosphorus indicated that salinity best predicts the toxic bloom occurrence. Minimum estimated salinities for toxic bloom formation were 0.59 and 1.02 psu in Brazos and Colorado River reservoirs, respectively. Principal component analysis (POR, 2001-2010) indicated that GA habitat is best defined by higher salinity relative to nonimpacted reservoirs, with winter DO and pH also being slightly higher and winter temperature slightly lower in impacted reservoirs. Trend analysis, however, did not reveal monotonic changes in winter water quality of GA-impacted reservoirs during the 20-year period (1991-2010) bracketing the 2001 dispersal. Therefore, whereas minimum levels of salinity are required for GA establishment and toxic blooms in Texas reservoirs, the lack of trends in water quality suggests that conditions favorable for toxic blooms pre-date the 2001 expansion. These observations are consistent with a climate change-independent scenario of past GA dispersals in Texas reservoirs driven by novel introductions into pre-existing favorable habitat. Reports of latent GA populations in certain nonimpacted reservoirs, however, provide a plausible scenario of future dispersals characterized by prolonged periods between colonization and toxic bloom development and driven by changes in water quality, natural, or anthropogenic.

Beyond temperature: Clumped isotope signatures in dissolved inorganic carbon species and the influence of solution chemistry on carbonate mineral composition

USGS Publications Warehouse

Tripati, Aradhna K.; Hill, Pamela S.; Eagle, Robert A.; Mosenfelder, Jed L.; Tang, Jianwu; Schauble, Edwin A.; Eiler, John M.; Zeebe, Richard E.; Uchikawa, Joji; Coplen, Tyler B.; Ries, Justin B.; Henry, Drew

2015-01-01

“Clumped-isotope” thermometry is an emerging tool to probe the temperature history of surface and subsurface environments based on measurements of the proportion of 13C and 18O isotopes bound to each other within carbonate minerals in 13C18O16O22- groups (heavy isotope “clumps”). Although most clumped isotope geothermometry implicitly presumes carbonate crystals have attained lattice equilibrium (i.e., thermodynamic equilibrium for a mineral, which is independent of solution chemistry), several factors other than temperature, including dissolved inorganic carbon (DIC) speciation may influence mineral isotopic signatures. Therefore we used a combination of approaches to understand the potential influence of different variables on the clumped isotope (and oxygen isotope) composition of minerals.We conducted witherite precipitation experiments at a single temperature and at varied pH to empirically determine 13C-18O bond ordering (Δ47) and δ18O of CO32- and HCO3- molecules at a 25 °C equilibrium. Ab initio cluster models based on density functional theory were used to predict equilibrium 13C-18O bond abundances and δ18O of different DIC species and minerals as a function of temperature. Experiments and theory indicate Δ47 and δ18O compositions of CO32- and HCO3- ions are significantly different from each other. Experiments constrain the Δ47-δ18O slope for a pH effect (0.011 ± 0.001; 12 ⩾ pH ⩾ 7). Rapidly-growing temperate corals exhibit disequilibrium mineral isotopic signatures with a Δ47-δ18O slope of 0.011 ± 0.003, consistent with a pH effect.Our theoretical calculations for carbonate minerals indicate equilibrium lattice calcite values for Δ47 and δ18O are intermediate between HCO3− and CO32−. We analyzed synthetic calcites grown at temperatures ranging from 0.5 to 50 °C with and without the enzyme carbonic anhydrase present. This enzyme catalyzes oxygen isotopic exchange between DIC species and is present in many natural systems. The two types of experiments yielded statistically indistinguishable results, and these measurements yield a calibration that overlaps with our theoretical predictions for calcite at equilibrium. The slow-growing Devils Hole calcite exhibits Δ47 and δ18O values consistent with lattice equilibrium.Factors influencing DIC speciation (pH, salinity) and the timescale for DIC equilibration, as well as reactions at the mineral–solution interface, have the potential to influence clumped-isotope signatures and the δ18O of carbonate minerals. In fast-growing carbonate minerals, solution chemistry may be an important factor, particularly over extremes of pH and salinity. If a crystal grows too rapidly to reach an internal equilibrium (i.e., achieve the value for the temperature-dependent mineral lattice equilibrium), it may record the clumped-isotope signature of a DIC species (e.g., the temperature-dependent equilibrium of HCO3−) or a mixture of DIC species, and hence record a disequilibrium mineral composition. For extremely slow-growing crystals, and for rapidly-grown samples grown at a pH where HCO3- dominates the DIC pool at equilibrium, effects of solution chemistry are likely to be relatively small or negligible. In summary, growth environment, solution chemistry, surface equilibria, and precipitation rate may all play a role in dictating whether a crystal achieves equilibrium or disequilibrium clumped-isotope signatures.

Ultra high pressure homogenization (UHPH) inactivation of Bacillus amyloliquefaciens spores in phosphate buffered saline (PBS) and milk

PubMed Central

Dong, Peng; Georget, Erika S.; Aganovic, Kemal; Heinz, Volker; Mathys, Alexander

2015-01-01

Ultra high pressure homogenization (UHPH) opens up new areas for dynamic high pressure assisted thermal sterilization of liquids. Bacillus amyloliquefaciens spores are resistant to high isostatic pressure and temperature and were suggested as potential surrogate for high pressure thermal sterilization validation. B. amyloliquefaciens spores suspended in PBS buffer (0.01 M, pH 7.0), low fat milk (1.5%, pH 6.7), and whole milk (3.5%, pH 6.7) at initial concentration of ~106 CFU/mL were subjected to UHPH treatments at 200, 300, and 350 MPa with an inlet temperature at ~80°C. Thermal inactivation kinetics of B. amyloliquefaciens spores in PBS and milk were assessed with thin wall glass capillaries and modeled using first-order and Weibull models. The residence time during UHPH treatments was estimated to determine the contribution of temperature to spore inactivation by UHPH. No sublethal injury was detected after UHPH treatments using sodium chloride as selective component in the nutrient agar medium. The inactivation profiles of spores in PBS buffer and milk were compared and fat provided no clear protective effect for spores against treatments. Treatment at 200 MPa with valve temperatures lower than 125°C caused no reduction of spores. A reduction of 3.5 log10CFU/mL of B. amyloliquefaciens spores was achieved by treatment at 350 MPa with a valve temperature higher than 150°C. The modeled thermal inactivation and observed inactivation during UHPH treatments suggest that temperature could be the main lethal effect driving inactivation. PMID:26236296

Salinity affects behavioral thermoregulation in a marine decapod crustacean

NASA Astrophysics Data System (ADS)

Reiser, Stefan; Mues, Annika; Herrmann, Jens-Peter; Eckhardt, André; Hufnagl, Marc; Temming, Axel

2017-10-01

Thermoregulation in aquatic ectotherms is a complex behavioral pattern that is affected by various biotic and abiotic factors with one being salinity. Especially in coastal and estuarine habitats, altering levels of salinity involve osmoregulatory adjustments that affect total energy budgets and may influence behavioral responses towards temperature. To examine the effect of salinity on behavioral thermoregulation in a marine evertebrate ectotherm, we acclimated juvenile and sub-adult common brown shrimp (Crangon crangon, L.) to salinities of 10, 20 and 30 PSU and investigated their thermal preference in an annular chamber system using the gravitational method for temperature preference determination. Thermal preference of individual brown shrimp was considerably variable and brown shrimp selected a wide range of temperatures in each level of salinity as well as within individual experimental trials. However, salinity significantly affected thermal preference with the shrimp selecting higher temperatures at 10 and 20 PSU when compared to 30 PSU of salinity. Body size had no effect on thermal selection and did not interact with salinity. Temperature preference differed by sex and male shrimp selected significantly higher temperatures at 10 PSU when compared to females. The results show that salinity strongly affects thermal selection in brown shrimp and confirms the strong interrelation of temperature and salinity on seasonal migratory movements that has been previously derived from observations in the field. In the field, however, it remains unclear whether salinity drives thermal selection or whether changes in temperature modify salinity preference.

Effect of reclamation of abandoned salinized farmland on soil bacterial communities in arid northwest China.

PubMed

Cheng, Zhibo; Chen, Yun; Zhang, Fenghua

2018-07-15

Understanding the impact of reclamation of abandoned salinized farmland on soil bacterial community is of great importance for maintaining soil health and sustainability in arid regions. In this study, we used field sampling and 454 pyrosequencing methods to investigate the effects of 5-year reclamation treatments on soil properties, bacterial community composition and diversity. The four reclamation treatments are: abandoned salinized farmland (CK), cropland (CL), grassland (GL) and woodland (WL). We have found soil properties are significantly altered by abandoned salinized farmland reclamation. In particular, the lowest soil pH and electrical conductivity (EC) values are observed in CL (P<0.05). The dominant phyla are Firmicutes, Proteobacteria, Chloroflexi, Actinobacteria and Acidobacteria in all treatments. At the genus levels, the relative abundance of Bacillus, Lactococcus, Streptococcus and Enterococcus in CK, GL and WL is significantly higher than in CL. Bacterial diversity indices (i.e. ACE, Chao and Shannon) dramatically increase after the reclamation, with the highest in CL. Similar patterns of bacterial communities have been observed in CK, GL and WL soils, but significantly different from CL. Regression analyses indicate that the relative abundance of these phyla are significantly correlated with soil Fe, pH and EC. Results from non-metric multidimensional scaling (NMDS) and redundancy analysis (RDA) indicate that soil Fe content, EC and pH are the most important factors in shaping soil bacterial communities. Overall, results indicate that abandoned salinized farmland reclaimed for CL significantly decrease soil pH and EC, and increase soil bacterial community diversity. Soil Fe concentration, EC and pH are the dominant environmental factors affecting soil bacterial community composition. The important role of Fe concentration in shaping bacterial community composition is a new discovery among the similar studies. Copyright © 2018. Published by Elsevier B.V.

Benthic infaunal community structuring in an acidified tropical estuarine system.

PubMed

Hossain, M Belal; Marshall, David J

2014-01-01

Recent studies suggest that increasing ocean acidification (OA) should have strong direct and indirect influences on marine invertebrates. While most theory and application for OA is based on relatively physically-stable oceanic ecological systems, less is known about the effects of acidification on nearshore and estuarine systems. Here, we investigated the structuring of a benthic infaunal community in a tropical estuarine system, along a steep salinity and pH gradient, arising largely from acid-sulphate groundwater inflows (Sungai Brunei Estuary, Borneo, July 2011- June 2012). Preliminary data indicate that sediment pore-water salinity (range: 8.07 - 29.6 psu) declined towards the mainland in correspondence with the above-sediment estuarine water salinity (range: 3.58 - 31.2 psu), whereas the pore-water pH (range: 6.47- 7.72) was generally lower and less variable than the estuarine water pH (range: 5.78- 8.3), along the estuary. Of the thirty six species (taxa) recorded, the polychaetes Neanthes sp., Onuphis conchylega, Nereididae sp. and the amphipod Corophiidae sp., were numerically dominant. Calcified microcrustaceans (e.g., Cyclopoida sp. and Corophiidae sp.) were abundant at all stations and there was no clear distinction in distribution pattern along the estuarine between calcified and non-calcified groups. Species richness increased seawards, though abundance (density) showed no distinct directional trend. Diversity indices were generally positively correlated (Spearman's rank correlation) with salinity and pH (p <0.05) and negatively with clay and organic matter, except for evenness values (p >0.05). Three faunistic assemblages were distinguished: (1) nereid-cyclopoid-sabellid, (2) corophiid-capitellid and (3) onuphid- nereid-capitellid. These respectively associated with lower salinity/pH and a muddy bottom, low salinity/pH and a sandy bottom, and high salinity/pH and a sandy bottom. However, CCA suggested that species distribution and community structuring is more strongly influenced by sediment particle characteristics than by the chemical properties of the water (pH and salinity). Infaunal estuarine communities, which are typically adapted to survive relatively acidic conditions, may be less exposed, less sensitive, and less vulnerable than epibenthic or pelagic communities to further acidification of above-sediment waters. These data question the extent to which all marine infaunal communities, including oceanic communities, are likely to be affected by future global CO2-driven acidification.

New lipopeptide produced by Corynebacterium aquaticum from a low-cost substrate.

PubMed

Martins, Paola Chaves; Bastos, Cibele Garcia; Granjeiro, Paulo Afonso; Martins, Vilásia Guimarães

2018-04-26

Conventional biosurfactants have high production costs. Therefore, the use of low-cost carbon sources for their production is attractive for industry. The ability to remain stable under various environmental conditions further extends industrial application. Here we aimed to evaluate the stability of a new lipopeptide produced by Corynebacterium aquaticum using fish residue as an unconventional energy source. The biosurfactant was produced using 3% fish residue, 2% of the microorganism, and mineral medium. Biosurfactant characterization was performed by thin layer chromatography (TLC), as well as by testing its infrared, surface tension, emulsifying activity, and ionic character. The stability of the biosurfactant was evaluated by testing its surface tension at a range of temperatures, pH, and saline concentrations, as well as after 6 months of storage. The biosurfactant was characterized as a lipopeptide due to its retention time, which was coincident with the amino acid and lipid chains obtained in the TLC analysis, being confirmed by some regions of absorption verified in the infrared analysis. The surface tension and emulsifying activity of the biosurfactant were 27.8 mN/m and 87.6%, respectively, and showed anionic character. The biosurfactant was stable at temperatures of 20 to 121 °C, in saline concentrations of 1 to 7%, and at pH close to neutrality. Based on our findings, it is possible to use unconventional sources of energy to produce a lipopeptide biosurfactant that can act under various environments.

Marine water quality under climate change conditions/scenarios

NASA Astrophysics Data System (ADS)

Rizzi, Jonathan; Torresan, Silvia; Critto, Andrea; Zabeo, Alex; Brigolin, Daniele; Carniel, Sandro; Pastres, Roberto; Marcomini, Antonio

2016-04-01

The increase of sea temperature and the changes in marine currents are generating impacts on coastal waters such as changes in water biogeochemical and physical parameters (e.g. primary production, pH, salinity) leading to progressive degradation of the marine environment. With the main aim of analysing the potential impacts of climate change on coastal water quality, a Regional Risk Assessment (RRA) methodology was developed and applied to coastal marine waters of the North Adriatic (i.e. coastal water bodies of the Veneto and Friuli Venezia Giulia regions, Italy). RRA integrates the outputs of regional models providing information on macronutrients (i.e. dissolved inorganic nitrogen e reactive phosphorus), dissolved oxygen, pH, salinity and temperature, etc., under future climate change scenarios with site-specific environmental and socio-economic indicators (e.g. biotic index, presence and extension of seagrasses, presence of aquaculture). The presented approach uses Geographic Information Systems to manage, analyse, and visualize data and employs Multi-Criteria Decision Analysis for the integration of stakeholders preferences and experts judgments into the evaluation process. RRA outputs are hazard, exposure, vulnerability, risk and damage maps useful for the identification and prioritization of hot-spot areas and vulnerable targets in the considered region. Therefore, the main aim of this contribution is to apply the RRA methodology to integrate, visualize, and rank according to spatial distribution, physical and chemical data concerning the coastal waters of the North Adriatic Sea in order to predict possible changes of the actual water quality.

Monitoring of impact of anthropogenic inputs on water quality of mangrove ecosystem of Uran, Navi Mumbai, west coast of India.

PubMed

Pawar, Prabhakar R

2013-10-15

Surface water samples were collected from substations along Sheva creek and Dharamtar creek mangrove ecosystems of Uran (Raigad), Navi Mumbai, west coast of India. Water samples were collected fortnightly from April 2009 to March 2011 during spring low and high tides and were analyzed for pH, Temperature, Turbidity, Total solids (TS), Total dissolved solids (TDS), Total suspended solids (TSS), Dissolved oxygen (DO), Biochemical oxygen demand (BOD), Carbon dioxide (CO2), Chemical oxygen demand (COD), Salinity, Orthophosphate (O-PO4), Nitrite-nitrogen (NO2-N), Nitrate-nitrogen (NO3-N), and Silicates. Variables like pH, turbidity, TDS, salinity, DO, and BOD show seasonal variations. Higher content of O-PO4, NO3-N, and silicates is recorded due to discharge of domestic wastes and sewage, effluents from industries, oil tanking depots and also from maritime activities of Jawaharlal Nehru Port Trust (JNPT), hectic activities of Container Freight Stations (CFS), and other port wastes. This study reveals that water quality from mangrove ecosystems of Uran is deteriorating due to industrial pollution and that mangrove from Uran is facing the threat due to anthropogenic stress. Copyright © 2013 Elsevier Ltd. All rights reserved.

Salinity and temperature tolerance of an emergent alien species, the Amazon fish Astronotus ocellatus

USGS Publications Warehouse

Gutierrel, Silvia M M; Schofield, Pam; Prodocimo, Viviane

2016-01-01

Astronotus ocellatus (oscar), is native to the Amazon basin and, although it has been introduced to many countries, little is known regarding its tolerances for salinity and temperature. In this report, we provide data on the tolerance of A. ocellatus to abrupt and gradual changes in salinity, its high and low temperature tolerance, and information on how salinity, temperature, and fish size interact to affect survival. Fish were able to survive abrupt transfer to salinities as high as 16 ppt with no mortality. When salinity change was gradual (2 ppt/day), fish in the warm-temperature experiment (28°C) survived longer than fish in the cool-temperature experiment (18°C). Larger fish survived longer than smaller ones at the higher salinities when the temperature was warm, but when the temperature was cool fish size had little effect on survival. In the temperature-tolerance experiments, fish survived from 9 to 41°C for short periods of time. Overall, the species showed a wide range of temperature and salinity tolerance. Thus, in spite of the tropical freshwater origin of this species, physiological stress is not likely to hinder its dispersal to brackish waters, especially when temperatures are warm.

Effects of Inhibition Conditions on Anammox process

NASA Astrophysics Data System (ADS)

Xie, Haitao; Ji, Dandan; Zang, Lihua

2017-12-01

Anaerobic ammonium oxidation (Anammox) is a very suitable process for the treatment of nitrogen-rich wastewater, which is a promising new biological nitrogen removal process, and has a good application prospects. However, the Anammox process is inhibited by many factors, which hinders the process improvement and the application of the Anammox process. Such as organic,temperature,salts,heavy metals, phosphates, sulfides, pH and other inhibitors are usually present in practical applications. We have reviewed the previous researches on the inhibition of Anammox processes. The effect of the substrate on the anaerobic oxide is mainly caused by free ammonia or nitrite nitrogen. Most heavy metals inhibit Anammox growth and activity. The inhibition of organic matter depends on the content of organic matter and species. High salinity inhibits Anammox activity. Dissolved oxygen allows the flora to be in a balanced state. The optimum pH and temperature, as well as other factors, can provide a good growth environment for Anammox. The knowledge of inhibition on Anammox will help prevent the application and improvement of the Anammox process.

Meta-Cresol Purple Reference Material® (RM) for Seawater pH Measurements

NASA Astrophysics Data System (ADS)

Easley, R. A.; Waters, J. F.; Place, B. J.; Pratt, K. W.

2016-02-01

The pH of seawater is a fundamental quantity that governs the carbon dioxide - carbonate system in the world's oceans. High quality pH measurements for long-term monitoring, shipboard studies, and shorter-term biological studies (mesocosm and field experiments) can be ensured through a reference material (RM) that is compatible with existing procedures and which is traceable to primary pH measurement metrology. High-precision spectrophotometric measurements of seawater pH using an indicator dye such as meta-cresol purple (mCP) are well established. However, traceability of these measurements to the International System of Units (SI) additionally requires characterizing the spectrophotometric pH response of the dye in multiple artificial seawater buffers that themselves are benchmarked via primary pH (Harned cell) measurements at a range of pH, salinity, and temperature. NIST is currently developing such a mCP pH RM using this approach. This material will also incorporate new procedures developed at NIST for assessing the purity and homogeneity of the mCP reagent itself. The resulting mCP will provide long-term (years) stability and ease of shipment compared to artificial seawater pH buffers. These efforts will provide the oceanographic user community with a NIST issued mCP (RM), characterized as to its molar absorptivity values and acid dissociation constants (pKa), with uncertainties that comply with the Guide to the Expression of Uncertainty in Measurement (GUM).

Effects of variations in flow characteristics through W.P. Franklin Lock and Dam on downstream water quality in the Caloosahatchee River Estuary and in McIntyre Creek in the J.N. “Ding” Darling National Wildlife Refuge, southern Florida, 2010–13

USGS Publications Warehouse

Booth, Amanda C.; Soderqvist, Lars E.; Knight, Travis M.

2016-05-17

The U.S. Geological Survey studied water-quality trends at the mouth of McIntyre Creek, an entry point to the J.N. “Ding” Darling National Wildlife Refuge, to investigate correlations between flow rates and volumes through the W.P. Franklin Lock and Dam and water-quality constituents inside the refuge from March 2010 to December 2013. Outflow from Lake Okeechobee, and flows from Franklin Lock, tributaries to the Caloosahatchee River Estuary, and the Cape Coral canal system were examined to determine the sources and quantity of water to the study area. Salinity, temperature, dissolved-oxygen concentration, pH, turbidity, and chromophoric dissolved organic matter fluorescence (FDOM) were measured during moving-boat surveys and at a fixed location in McIntyre Creek. Chlorophyll fluorescence was also recorded in McIntyre Creek. Water-quality surveys were completed on 20 dates between 2011 and 2014 using moving-boat surveys.Franklin Lock contributed the majority of flow to the Caloosahatchee River. Between 2010 and 2013, the monthly mean flow rate at Franklin Lock ranged from 29 cubic feet per second in May 2011 to 10,650 cubic feet per second in August 2013. Instantaneous near-surface salinity in McIntyre Creek ranged from 12.9 parts per thousand on September 26, 2013, to 37.9 parts per thousand on June 27, 2011. Salinity in McIntyre Creek decreased with increasing flow rate through Franklin Lock. Flow rates through Franklin Lock explained 61 percent of the variation in salinity in McIntyre Creek. Salinity data from moving-boat surveys also indicate that an increase in flow rate at Franklin Lock decreases salinity in the Caloosahatchee River Estuary, and a reduction or elimination in flow increases salinity. The FDOM in McIntyre Creek was positively correlated with flow at Franklin Lock, and 54 percent of the variation in FDOM can be attributed to the flow rate through Franklin Lock. Data from moving-boat surveys indicate that FDOM increases when flow volume from Franklin Lock increases. The highest FDOM recorded during a survey was at Billy’s Creek. Chlorophyll fluorescence was positively correlated with flow at Franklin Lock, with 23 percent of the variation explained by the flow rate at Franklin Lock. An increase in flow rate at Franklin Lock resulted in a decrease in pH (21 percent of variation explained by flow rates). Data from the pH surveys indicate an increase in pH with distance from Franklin Lock. Turbidity and dissolved oxygen near the surface in McIntyre Creek were not correlated with flow rate at Franklin Lock. Moving-boat surveys did not document a change in turbidity or dissolved oxygen with a change in distance from the Franklin Lock. Correlations between Franklin Lock flow rate and water quality in McIntyre Creek indicate that releases at Franklin Lock affect water quality in the Caloosahatchee River Estuary and Ding Darling Refuge.

Kinetic model to explain the effect of ocean warming and acidification on the Fe(II) oxidation rate in oligotrophic and eutrophic natural waters

NASA Astrophysics Data System (ADS)

González-Dávila, M.; Samperio-Ramos, G.; Santana-Casiano, J. M.; Gonzallez, A. G.; Pérez-Almeida, N.

2016-12-01

The speciation of inorganic Fe(II) as a function of the pH and temperature have been modeled in order to elucidate the inorganic Fe(II) redox behavior over a wide range of scenarios of acidification and global warming of the upper ocean, as well as, changes due to natural ambient fluctuations of pH and temperature. In addition, a kinetic modeling approach has been carried out to elucidate the fractional contribution of most kinetically active Fe(II) species to the overall oxidation rate to improve our future and present knowledge with respect to redox iron chemistry in the marine systems. The kinetic model considers the interactions of Fe(II) with the major ions in seawater, including phosphate and silicate and the competition with copper with the ROS. The model has been applied to the experimental results in order to describe the effect of temperature and pH in the speciation of Fe(II) and to compute the fractional contribution of each Fe(II)-specie to the overall oxidation rate. The oxidation rates (kapp) of nanomolar levels of Fe(II) have been studied in seawater enriched with nutrients (SWEN) in air saturated conditions. The nutrient effect (nitrate, phosphate and silicate), on the oxidation of Fe(II), has been evaluated as a function of pH (7.2-8.2), temperature (5-35 ºC) and salinity (10-37.09). The oxidation of Fe(II) was faster in the presence of nutrient with the change in the Fe(II) oxidation rates (Δlogkapp) more intensive at higher temperatures over the entire pH range studied. From the model it can be observed that the inorganic speciation of Fe(II) is controlled largely by pH, either in SW or in SWEN. A greater presence of Fe-nutrient reactive species (FeH3SiO4+ and FePO4-) in SWEN at higher temperatures explained the changes in the oxidation process. The individual oxidation rates by oxygen, for the Fe(II) most kinetically active species (Fe2+, FeOH+, Fe(OH)2, FeCO3(OH)-, FeCO3, Fe(CO3)22-, FeH3SiO3+, FePO4-), were fitted as a function of the temperature.

Seasonal variations in physico-chemical characteristics of Tuticorin coastal waters, southeast coast of India

NASA Astrophysics Data System (ADS)

Balakrishnan, S.; Chelladurai, G.; Mohanraj, J.; Poongodi, J.

2017-07-01

Physico-chemical parameters were determined along the Vellapatti, Tharuvaikulam and Threspuram coastal waters, southeast coast of India. All the physico-chemical parameters such as sea surface temperature, salinity, pH, total alkalinity, total suspended solids, dissolved oxygen and nutrients like nitrate, nitrite, inorganic phosphate and reactive silicate were studied for a period of 12 months (June 2014-May 2015). Sea surface temperature varied from 26.4 to 29.7 °C. Salinity varied from 26.1 and 36.2 ‰, hydrogen ion concentration ranged between 8.0 and 8.5. Variation in dissolved oxygen content was from 4.125 to 4.963 mg l-1. Total alkalinity ranged from 64 to 99 mg/l. Total suspended solids ranged from 24 to 97 mg/l. Concentrations of nutrients, viz. nitrates (2.047-4.007 μM/l), nitrites (0.215-0.840 μM/l), phosphates (0.167-0.904 µM/l), total phosphorus (1.039-3.479 μM/l), reactive silicates (3.737-8.876 μM/l) ammonia (0.078-0.526 μM/l) and also varied independently.

Results and evaluation of a pilot primary monitoring network, San Francisco Bay, California, 1978

USGS Publications Warehouse

Bradford, W.L.; Iwatsubo, R.T.

1980-01-01

A primary monitoring network of 12 stations, with measurements at 1-meter depth intervals every 2 weeks during periods of high inflow from the Sacramento-San Joaquin River delta, and every 4-6 weeks during seasonal low delta inflows, appears adequate to observe major changes in ambient water quality in San Francisco Bay. A 1-year study tested the network operation and determined that analysis of the data could demonstrate the major changes in salinity, temperature, and light-attenuation distributions known to occur, based on earlier research, in response to variations of delta inflow and to other physical processes. Observations of eddies at two stations, of the influence of water from a river flooding in the extreme south bay, and of difference in salinity and temperature laterally across the entrance to the south bay are all new but are consistent with existing models. The pH, dissolved oxygen, and light-attenuation measurements, while adequate to observe small-scale vertical variations, are not sufficiently sensitive to detect the effects of phytoplankton blooms. (USGS)

Effects of temperature and salinity on survival, growth and DNA methylation of juvenile Pacific abalone, Haliotis discus hannai Ino

NASA Astrophysics Data System (ADS)

Kong, Ning; Liu, Xiao; Li, Junyuan; Mu, Wendan; Lian, Jianwu; Xue, Yanjie; Li, Qi

2017-09-01

Temperature and salinity are two of the most potent abiotic factors influencing marine mollusks. In this study, we investigated the individual and combined effects of temperature and salinity on the survival and growth of juvenile Pacific abalone, Haliotis discus hannai Ino, and also examined the DNA methylation alteration that may underpin the phenotypic variation of abalone exposed to different rearing conditions. The single-factor data showed that the suitable ranges of temperature and salinity were 16-28°C at a constant salinity of 32, and 24-40 at a constant temperature of 20°C, respectively. The two-factor data indicated that both survival and growth were significantly affected by temperature, salinity and their interaction. The optimal temperature-salinity combination for juveniles was 23-25°C and 30-36. To explore environment-induced DNA methylation alteration, the methylation-sensitive amplified polymorphism (MSAP) technique was used to analyze the genomic methylation profiles of abalone reared in optimal and adverse conditions. Neither temperature nor salinity induced evident changes in the global methylation level, but 67 and 63 differentially methylated loci were identified in temperature and salinity treatments, respectively. The between-group eigen analysis also showed that both temperature and salinity could induce epigenetic differentiation in H. discus hannai Ino. The results of our study provide optimal rearing conditions for juvenile H. discus hannai Ino, and represent the first step toward revealing the epigenetic regulatory mechanism of abalone in response to thermal and salt stresses.

Treatment and desalination of domestic wastewater for water reuse in a four-chamber microbial desalination cell.

PubMed

Lu, Yaobin; Abu-Reesh, Ibrahim M; He, Zhen

2016-09-01

Microbial desalination cells (MDCs) have been studied for contaminant removal from wastewater and salinity reduction in saline water. However, in an MDC wastewater treatment and desalination occurs in different streams, and high salinity of the treated wastewater creates challenges for wastewater reuse. Herein, a single-stream MDC (SMDC) with four chambers was developed for simultaneous organic removal and desalination in the same synthetic wastewater. This SMDC could achieve a desalination rate of 12.2-31.5 mg L(-1) h(-1) and remove more than 90 % of the organics and 75 % of NH4 (+)-N; the pH imbalance between the anode and cathode chambers was also reduced. Several strategies such as controlling catholyte pH, increasing influent COD concentration, adopting the batch mode, applying external voltage, and increasing the alkalinity of wastewater were investigated for improving the SMDC performance. Under a condition of 0.4 V external voltage, anolyte pH adjustment, and a batch mode, the SMDC decreased the wastewater salinity from 1.45 to below 0.75 mS cm(-1), which met the salinity standard of wastewater for irrigation. Those results encourage further development of the SMDC technology for sustainable wastewater treatment and reuse.

Enhanced oil recovery by nitrogen and carbon dioxide injection followed by low salinity water flooding for tight carbonate reservoir: experimental approach

NASA Astrophysics Data System (ADS)

Georges Lwisa, Essa; Abdulkhalek, Ashrakat R.

2018-03-01

Enhanced Oil Recovery techniques are one of the top priorities of technology development in petroleum industries nowadays due to the increase in demand for oil and gas which cannot be equalized by the primary production or secondary production methods. The main function of EOR process is to displace oil to the production wells by the injection of different fluids to supplement the natural energy present in the reservoir. Moreover, these injecting fluids can also help in the alterations of the properties of the reservoir like lowering the IFTs, wettability alteration, a change in pH value, emulsion formation, clay migration and oil viscosity reduction. The objective of this experiment is to investigate the residual oil recovery by combining the effects of gas injection followed by low salinity water injection for low permeability reservoirs. This is done by a series of flooding tests on selected tight carbonate core samples taken from Zakuum oil field in Abu Dhabi by using firstly low salinity water as the base case and nitrogen & CO2injection followed by low salinity water flooding at reservoir conditions of pressure and temperature. The experimental results revealed that a significant improvement of the oil recovery is achieved by the nitrogen injection followed by the low salinity water flooding with a recovery factor of approximately 24% of the residual oil.

Light Scattering Characterization of Elastin-Like Polypeptide Trimer Micelles

NASA Astrophysics Data System (ADS)

Tsuper, Ilona; Terrano, Daniel; Maraschky, Adam; Holland, Nolan; Streletzky, Kiril

The elastin-like polypeptides (ELP) nanoparticles are composed of three-armed star polypeptides connected by a negatively charged foldon. Each of the three arms extending from the foldon domain includes 20 repeats of the (GVGVP) amino acid sequence. The ELP polymer chains are soluble at room temperature and become insoluble at the transition temperature (close to 50 ° C), forming micelles. The size and shape of the micelle are dependent on the temperature and the pH of the solution, and on the concentration of the phosphate buffered saline (PBS). The depolarized dynamic light scattering (DDLS) was employed to study the structure and dynamics of micelles at 62 ° C. The solution was maintained at an approximate pH level of 7.3 - 7.5, while varying PBS concentration. At low salt concentrations (<15 mM), the micelle radius was about 10nm but not very reproducible on account of unstable pH levels arising from low buffer concentrations. At intermediate salt concentrations (15 - 60 mM), the system formed spherically-shaped micelles, exhibiting a steady growth in the hydrodynamic radius (Rh) from 10 to 21 nm, with increasing PBS concentration. Interestingly, higher salt concentrations (>60 mM) displayed an apparent elongation of the micelles evident by a significant VH signal, along with a surge in the apparent Rh. A model of micelle growth (and potential elongation) with increase in salt concentration is considered.

The effects of heated and room-temperature abdominal lavage solutions on core body temperature in dogs undergoing celiotomy.

PubMed

Nawrocki, Michael A; McLaughlin, Ron; Hendrix, P K

2005-01-01

To document the magnitude of temperature elevation obtained with heated lavage solutions during abdominal lavage, 18 dogs were lavaged with sterile isotonic saline intraoperatively (i.e., during a celiotomy). In nine dogs, room-temperature saline was used. In the remaining nine dogs, saline heated to 43+/-2 degrees C (110+/-4 degrees F) was used. Esophageal, rectal, and tympanic temperatures were recorded every 60 seconds for 15 minutes after initiation of the lavage. Temperature levels decreased in dogs lavaged with room-temperature saline. Temperature levels increased significantly in dogs lavaged with heated saline after 2 to 6 minutes of lavage, and temperatures continued to increase throughout the 15-minute lavage period.

Recent Molecular Advances on Downstream Plant Responses to Abiotic Stress

PubMed Central

dos Reis, Sávio Pinho; Lima, Aline Medeiros; de Souza, Cláudia Regina Batista

2012-01-01

Abiotic stresses such as extremes of temperature and pH, high salinity and drought, comprise some of the major factors causing extensive losses to crop production worldwide. Understanding how plants respond and adapt at cellular and molecular levels to continuous environmental changes is a pre-requisite for the generation of resistant or tolerant plants to abiotic stresses. In this review we aimed to present the recent advances on mechanisms of downstream plant responses to abiotic stresses and the use of stress-related genes in the development of genetically engineered crops. PMID:22942725

Detecting crop yield reduction due to irrigation-induced soil salinization in South-West Russia

NASA Astrophysics Data System (ADS)

Argaman, E.; Beets, W.; Croes, J.; Keesstra, S.; Verzandvoort, S.; Zeiliguer, A.

2012-04-01

The South-European part of the Russian Federation has experienced serious land degradation in the form of soil salinization since the 1960s. This land degradation was caused by intensive, large-scale irrigation on reclaimed land in combination with the salt-rich nature of the substrate. Alkaline soil salinity is believed to be an important factor decreasing crop yield in this area. A large research effort has been directed to the effects of soil salinity on crops, there is a need for simple, easily determinable indicators of crop health and soil salinity in irrigated systems, that can help to detect crop water stress in an early stage. The objectives of this research were to study the effects of soil salinity and vegetation water stress on the performance of alfalfa crop yield and physiological crop properties, and to study the possibility to measure soil salinity and alkalinity and the crop water stress index at plot level using a thermal gun and a regular digital camera. The study area was located in Saratov District, in the South-West part of Russia. Variables on the surface energy balance, crop properties, soil properties and visible reflectance were measured on plots with alfalfa cultures in two fields with and without signs of alkaline soil salinity, and with and without irrigation in July 2009. The research showed no clear adverse effects of soil salinity and soil alkalinity on crop yield and physiological crop properties. Soil salinity, as reflected by the electric conductivity, positively affected the root biomass of alfalfa in the range of 0.15 to 1.52 dS/m . This was a result of EC levels being below the documented threshold to negatively affect Alfalfa, as would be the case in truly saline soils. The soil pH also showed a positive correlation with root biomass within the range of pH 6.2 and 8.5 . From the literature these pH values are generally believed to be too high to exhibit a positive relationship with root biomass. No relationship was found between EC and pH on the one hand , and soil moisture content on the other. However, soil moisture content in the topsoil appeared to have a major influence on the crop water stress index, which on its turn affected the leaf area index, the fresh biomass and the mean plant height. The crop leaf color as detected by a regular digital camera appeared to be correlated with pH and EC properties of the soil. The visible light band ratios red/green and blue/green correlated well with the crop water stress index. More research is necessary to prove if this relation is applicable in different environments, and for different crops. A confirmation of these findings would offer scope to increase the spatial support of this technique using satellite images.

Effects of pH, dissolved organic matter, and salinity on ibuprofen sorption on sediment.

PubMed

Oh, Sanghwa; Shin, Won Sik; Kim, Hong Tae

2016-11-01

Ibuprofen is well known as one of the most frequently detected pharmaceuticals and personal care products (PPCPs) in rivers. However, sorption of ibuprofen onto sediment has not been considered in spite of its high K ow (3.5). In this study, the effects of various environmental conditions such as pH (4, 5.3, and 7), the concentrations of dissolved organic matters (0 to 1.0 mM citrate and urea), salinity (0, 10, 20, and 30 part per thousand), and presence of other PPCP (salicylic acid) on ibuprofen sorption were investigated. Linear model mainly fitted the experimental data for analysis. The distribution coefficient (K d ) in the linear model decreased from 6.76 at pH 4 to near zero at pH 7, indicating that neutral form of ibuprofen at pH below pKa (5.2) was easily sorbed onto the sediment whereas the sorption of anionic form at pH over pKa was not favorable. To investigate the effect of dissolved organic matters (DOMs) on ibuprofen sorption, citrate and urea were used as DOMs. As citrate concentration increased, the K d value decreased but urea did not interrupt the ibuprofen sorption. Citrate has three carboxyl functional groups which can attach easily ibuprofen and hinder its sorption onto sediment. Salinity also affected ibuprofen sorption due to decrease of the solubility of ibuprofen as salinity increased. In competitive sorption experiment, the addition of salicylic acid also led to enhance ibuprofen sorption. Conclusively, ibuprofen can be more easily sorbed onto the acidified sediments of river downstream, especially estuaries or near-shore environment with low DOM concentration.

A microbiological survey of Montserrat Island hydrothermal biotopes.

PubMed

Atkinson, T; Cairns, S; Cowan, D A; Danson, M J; Hough, D W; Johnson, D B; Norris, P R; Raven, N; Robinson, C; Robson, R; Sharp, R J

2000-10-01

In March 1996, a survey of hydrothermal sites on the island of Montserrat was carried out. Six sites (Galway's Soufrière. Gages Upper and Lower Soufrières, Hot Water Pond, Hot River, and Tar River Soufrière) were mapped and sampled for chemical, ATP, and microbial analyses. The hydrothermal Soufrière sites on the slopes of the active Chances Peak volcano exhibited temperatures up to almost 100 degrees C and were generally either mildly acidic at pH 5-7 or strongly acidic at pH 1.5-3, but with some hot streams and pools of low redox potential at pH 7-8. Hot Water Pond sites, comprising a series of heated pools near the western shoreline of the island. were neutral and saline, consistent with subsurface heating of entrained seawater. Biological activity shown by ATP analyses was greatest in near-neutral pH samples and generally decreased as acidity increased. A variety of heterotrophic and chemolithotrophic thermophilic organisms were isolated or observed in enrichment cultures. Most of the bacteria that were obtained in pure culture were familiar acidophiles and neutrophiles, but novel, iron-oxidizing species of Sulfobacillus were revealed. These species included the first mesophilic iron-oxidizing Sulfobacillus strains to be isolated and a strain with a higher maximum growth temperature (65 degrees C) than the previously described moderately thermophilic Sulfobacillus species.

Water-mass dynamics of an Arctic cold-water coral reef: First results from a new ocean observatory system

NASA Astrophysics Data System (ADS)

Flögel, Sascha; Karstensen, Johannes; Linke, Peter; Pfannkuche, Olaf; Ashastina, Kseniia; Dullo, Christian

2015-04-01

Cold-water coral reefs occur at various sites along the European continental margin, like in the Mediterranean Sea, on carbonate mounds West off Ireland, or at shallower depths between 100 and 350 m on the Norwegian shelf. Their occurrence is related to different physical parameters like temperature, salinity, seawater density, dissolved oxygen, and to other environmental parameters such as internal wave activity, nutrient supply, strong currents, which keep sediment input low, etc. Here, we present first results from a long-term observation in one of the nortnermost cold-water coral reefs at 70.5°N - the Stjernsund in northern Norway. The Stjernsund is a 30 km long and up to 3.5 km wide sound connecting the open North Atlantic with a fjord system. A deep-seated SW-NE oriented morainic sill with varying depths (203-236 m) splits the more than 400 m deep sound into two troughs. Living Lophelia pertusa dominated reef complexes occur on the NW slope between 235 and 305 m water depths and on the SE slope between 245 and 280 m. To investigate the dominating physical and biogeochemical boundary conditions a new modular seafloor observatory, MoLab, consisting of five sea-floor observatories and two moorings was deployed for 100 days during the summer of 2012. The various lander systems and moorimgs were equipped with sensors to measure current velocities and directions, temperature, salinity, pressure, pH, turbidity, fluorescence, oxygen concentration and saturation. Results showed that near-bottom salinities, temperature and current velocities are dominated by a semi-diurnal tidal forcing (pronounced M2 constituent), which cause vertical water mass movements of up to 100 m. These influence large parts of the living reef. Closer examination revealed overturning cells on the south-eastern slope of the sill during high tide, when Atlantic Water flows over the sill. The appearance of living cold-water corals is limited to a density envelope of sigma-theta=27.25-27.50 kg/m-3, which marks the boundary between Norwegian Coastal Water and Atlantic Water. Globally, Lophelia pertusa lives in waters covering a wide range of physical and biogeochemical parameters. This new data sets indicates parameter ranges, of e.g. current velocities (15-30 cm/s), temperature (6.0-6.8°C) and salinity (34.1-34.8), pH (8.22-7.39), turbidity (0.1-0.9 NTU), and oxygen concentration (300-339 μM) that are in agreement with other cold-water coral reefs in the NE Atlantic. The overall circulation depicts a complex dynamic system with pronounced differences not only vertically, but also important horizontal changes on top of the sill.

Photocatalytic degradation of humic acid in saline waters. Part 1. Artificial seawater: influence of TiO2, temperature, pH, and air-flow.

PubMed

Al-Rasheed, Radwan; Cardin, David J

2003-06-01

We report the first systematic study on the photocatalytic oxidation of humic acid (HA) in artificial seawater (ASW). TiO(2) (Degussa P25) dispersions were used as the catalyst with irradiation from a medium-pressure mercury lamp. The optimum quantity of catalyst was found to be between 2 and 2.5 gl(-1); while the decomposition was fastest at low pH values (pH 4.5 in the range examined), and the optimum air-flow, using an immersion well reactor with a capacity of 400 ml, was 850 ml min(-1). Reactivity increased with air-flow up to this figure, above which foaming prevented operation of the reactor. Using pure oxygen, an optimal flow rate was observed at 300 ml min(-1), above which reactivity remains essentially constant. Following treatment for 1 h, low-salinity water (2700 mg l(-1)) was completely mineralised, whereas ASW (46000 mg l(-1)) had traces of HA remaining. These effects are interpreted and kinetic data presented. To avoid problems of precipitation due to change of ionic strength humic substances were prepared directly in ASW, and the effects of ASW on catalyst suspension and precipitation have been taken into account. The Langmuir-Hinshelwood kinetic model has been shown to be followed only approximately for the catalytic oxidation of HA in ASW. The activation energy for the reaction derived from an Arrhenius treatment was 17 (+/-0.6) kJ mol(-1).

Gold nanoparticle incorporated polymer/bioactive glass composite for controlled drug delivery application.

PubMed

Jayalekshmi, A C; Sharma, Chandra P

2015-02-01

The present study discusses the development of a biodegradable polymer encapsulated-nanogold incorporated-bioactive glass composite (AuPBG) by a low-temperature method. The composite was analyzed by atomic force microscopy (AFM), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, thermogravimetry (TG), fluorescence and dissolution analysis. The composite exhibited aggregation behaviour in solid and solution states and exhibited negative zeta potential (-13.3 ± 1.4 mV). The composite exhibited fast degradation starting from the 5(th) day onwards in phosphate buffered saline (PBS) for a period of 14 days. The composite showed fluorescence quenching effect at pH 7 and the fluorescence recovered at pH 5. The composite has been found to be suitable for the release of doxorubicin at high rates at acidic pH (∼ 5) which is the intracellular pH of tumour cells. The drug loading ratio is also high and it exhibited a controlled release for a period of 8 days in PBS. The system serves as a promising material for targeted drug delivery applications. Copyright © 2014 Elsevier B.V. All rights reserved.

Influence of increasing temperature and salinity on herbicide toxicity in estuarine phytoplankton.

PubMed

DeLorenzo, Marie E; Danese, Loren E; Baird, Thomas D

2013-07-01

Ecological risk assessments are, in part, based on results of toxicity tests conducted under standard exposure conditions. Global climate change will have a wide range of effects on estuarine habitats, including potentially increasing water temperature and salinity, which may alter the risk assessment of estuarine pollutants. We examined the effects of increasing temperature and salinity on the toxicity of common herbicides (irgarol, diuron, atrazine, and ametryn) to the phytoplankton species Dunaliella tertiolecta. Static 96-h algal bioassays were conducted for each herbicide under four exposure scenarios: standard temperature and salinity (25°C, 20 ppt), standard temperature and elevated salinity (25°C, 40 ppt), elevated temperature and standard salinity (35°C, 20 ppt), and elevated temperature and elevated salinity (35°C, 40 ppt). The endpoints assessed were algal cell density at 96 h, growth rate, chlorophyll a content, lipid content, and starch content. Increasing exposure temperature reduced growth rate and 96-h cell density but increased the cellular chlorophyll and lipid concentrations of the control algae. Exposure condition did not alter starch content of control algae. Herbicides were found to decrease growth rate, 96 h cell density, and cellular chlorophyll and lipid concentrations, while starch concentrations increased with herbicide exposure. Herbicide effects under standard test conditions were then compared with those observed under elevated temperature and salinity. Herbicide effects on growth rate, cell density, and starch content were more pronounced under elevated salinity and temperature conditions. To encompass the natural variability in estuarine temperature and salinity, and to account for future changes in climate, toxicity tests should be conducted under a wider range of environmental conditions. Copyright © 2011 Wiley Periodicals, Inc.

A Water Tank Study of the Effects of Seawater Temperature on Coral Metabolism and Changes in Chemical Compositions in Seawater

NASA Astrophysics Data System (ADS)

Fujimura, H.; Arakaki, T.; Hamdun, A. M.; Oomori, T.

2002-12-01

For the past several years, large-scale coral bleaching has been observed in many coral reef areas around the world. Coral bleaching is considered to be caused mainly by high seawater temperature together with other factors such as strong UV-light and changes in salinity. However, the mechanism of coral bleaching is not clearly understood. We have conducted experiments using water tanks under well-controlled light and temperature conditions to elucidate the effects of seawater temperature on coral_fs metabolism and changes in chemical compositions in the seawater around the coral. Metabolism of coral was studied by analyzing changes in seawater chemical compositions. Coral specimen used in our experiment, Goniastrea aspera, was collected from northern shore of Okinawa island, Japan. pH, nitrate ion, dissolved organic carbon, and alkalinity were measured. Photochemically formed hydroxyl radical was also studied in those seawater samples.

Stability and tribological performances of fluid phospholipid bilayers: effect of buffer and ions.

PubMed

Dekkiche, F; Corneci, M C; Trunfio-Sfarghiu, A-M; Munteanu, B; Berthier, Y; Kaabar, W; Rieu, J-P

2010-10-15

We have investigated the mechanical and tribological properties of supported Dioleoyl phosphatidylcholine (DOPC) bilayers in different solutions: ultrapure water (pH 5.5), saline solution (150 mM NaCl, pH 5.8), Tris buffer (pH 7.2) and Tris saline buffer (150 mM NaCl, pH 7.2). Friction forces are measured using a homemade biotribometer. Lipid bilayer degradation is controlled in situ during friction tests using fluorescence microscopy. Mechanical resistance to indentation is measured by force spectroscopy with an atomic force microscope. This study confirms that mechanical stability under shear or normal load is essential to obtain low and constant friction coefficients. In ultrapure water, bilayers are not resistant and have poor lubricant properties. On the other hand, in Tris saline buffer, they fully resist to indentation and exhibit low (micro=0.035) and stable friction coefficient with no visible wear during the 50 min of the friction test. The unbuffered saline solution improves the mechanical resistance to indentation but not the lubrication. These results suggest that the adsorption of ions to the zwiterrionic bilayers has different effects on the mechanical and tribological properties of bilayers: higher resistance to normal indentation due to an increase in bilayer cohesion, higher lubrication due to an increase in bilayer-bilayer repulsion. Copyright (c) 2010 Elsevier B.V. All rights reserved.

A Mosaic of Geothermal and Marine Features Shapes Microbial Community Structure on Deception Island Volcano, Antarctica.

PubMed

Bendia, Amanda G; Signori, Camila N; Franco, Diego C; Duarte, Rubens T D; Bohannan, Brendan J M; Pellizari, Vivian H

2018-01-01

Active volcanoes in Antarctica contrast with their predominantly cold surroundings, resulting in environmental conditions capable of selecting for versatile and extremely diverse microbial communities. This is especially true on Deception Island, where geothermal, marine, and polar environments combine to create an extraordinary range of environmental conditions. Our main goal in this study was to understand how microbial community structure is shaped by gradients of temperature, salinity, and geochemistry in polar marine volcanoes. Thereby, we collected surface sediment samples associated with fumaroles and glaciers at two sites on Deception, with temperatures ranging from 0 to 98°C. Sequencing of the 16S rRNA gene was performed to assess the composition and diversity of Bacteria and Archaea. Our results revealed that Deception harbors a combination of taxonomic groups commonly found both in cold and geothermal environments of continental Antarctica, and also groups normally identified at deep and shallow-sea hydrothermal vents, such as hyperthermophilic archaea. We observed a clear separation in microbial community structure across environmental gradients, suggesting that microbial community structure is strongly niche driven on Deception. Bacterial community structure was significantly associated with temperature, pH, salinity, and chemical composition; in contrast, archaeal community structure was strongly associated only with temperature. Our work suggests that Deception represents a peculiar "open-air" laboratory to elucidate central questions regarding molecular adaptability, microbial evolution, and biogeography of extremophiles in polar regions.

Effects of temperature and salinity on survival rate of cultured corals and photosynthetic efficiency of zooxanthellae in coral tissues

NASA Astrophysics Data System (ADS)

Kuanui, Pataporn; Chavanich, Suchana; Viyakarn, Voranop; Omori, Makoto; Lin, Chiahsin

2015-06-01

This study investigated the effects of temperature and salinity on growth, survival, and photosynthetic efficiency of three coral species, namely, Pocillopora damicornis, Acropora millepora and Platygyra sinensis of different ages (6 and 18 months old). The experimental corals were cultivated via sexual propagation. Colonies were exposed to 5 different temperatures (18, 23, 28, 33, and 38°C) and 5 different salinities (22, 27, 32, 37, and 42 psu). Results showed that temperature significantly affected photosynthetic efficiency (Fv/Fm) (p < 0.05) compared to salinity. The maximum quantum yield of corals decreased ranging from 5% to 100% when these corals were exposed to different temperatures and salinities. Temperature also significantly affected coral growth and survival. However, corals exposed to changes in salinity showed higher survivorship than those exposed to changes in temperature. Results in this study also showed that corals of different ages and of different species did not display the same physiological responses to changes in environmental conditions. Thus, the ability of corals to tolerate salinity and temperature stresses depends on several factors.

Protective role of salt in catalysis and maintaining structure of halophilic proteins against denaturation.

PubMed

Sinha, Rajeshwari; Khare, Sunil K

2014-01-01

Search for new industrial enzymes having novel properties continues to be a desirable pursuit in enzyme research. The halophilic organisms inhabiting under saline/ hypersaline conditions are considered as promising source of useful enzymes. Their enzymes are structurally adapted to perform efficient catalysis under saline environment wherein n0n-halophilic enzymes often lose their structure and activity. Haloenzymes have been documented to be polyextremophilic and withstand high temperature, pH, organic solvents, and chaotropic agents. However, this stability is modulated by salt. Although vast amount of information have been generated on salt mediated protection and structure function relationship in halophilic proteins, their clear understanding and correct perspective still remain incoherent. Furthermore, understanding their protein architecture may give better clue for engineering stable enzymes which can withstand harsh industrial conditions. The article encompasses the current level of understanding about haloadaptations and analyzes structural basis of their enzyme stability against classical denaturants.

Protective role of salt in catalysis and maintaining structure of halophilic proteins against denaturation

PubMed Central

Sinha, Rajeshwari; Khare, Sunil K.

2014-01-01

Search for new industrial enzymes having novel properties continues to be a desirable pursuit in enzyme research. The halophilic organisms inhabiting under saline/ hypersaline conditions are considered as promising source of useful enzymes. Their enzymes are structurally adapted to perform efficient catalysis under saline environment wherein n0n-halophilic enzymes often lose their structure and activity. Haloenzymes have been documented to be polyextremophilic and withstand high temperature, pH, organic solvents, and chaotropic agents. However, this stability is modulated by salt. Although vast amount of information have been generated on salt mediated protection and structure function relationship in halophilic proteins, their clear understanding and correct perspective still remain incoherent. Furthermore, understanding their protein architecture may give better clue for engineering stable enzymes which can withstand harsh industrial conditions. The article encompasses the current level of understanding about haloadaptations and analyzes structural basis of their enzyme stability against classical denaturants. PMID:24782853

Occurance and survival of Vibrio alginolyticus in Tamouda Bay (Morocco).

PubMed

Sabir, M; Cohen, N; Boukhanjer, A; Ennaji, M M

2011-10-15

The objectives of this study were to investigate the spatial and seasonal fluctuations of Vibrio alginolyticus in marine environment of the Tamouda Bay on the Mediterranean coast of Morocco and to determine the dominant factors of the environment that govern these fluctuations. The samples (sea water, plankton, shellfish and sediment) were collected fortnightly for two years from three study sites on the coast Tamouda Bay in northern Morocco. The charge of Vibrio alginolyticus is determined by MPN method. The physicochemical parameters including temperature of sea water, pH, salinity, turbidity and chlorophyll a concentration were determined. Analysis of variance of specific variables and several principal component analyses showed that the temperature of seawater is the major determinant of seasonal distribution of Vibrio alginolyticus. The results showed a positive linear correlation between Vibrio alginolyticus and the water temperature, pH, turbidity and chlorophyll a. Similarly, there are seasonal variations and spatial of Vibrio alginolyticus in marine environment of the Tamouda bay and the highest concentrations were recorded in both years of study during the warm season whereas it was minimal during the cold season. Linear positive correlation was recorded between Vibrio alginolyticus populations in all ecological types of samples studied.

Volunteer revegetation of waste rock surfaces at the Bingham Canyon Mine, Utah.

PubMed

Borden, Richard K; Black, Rick

2005-01-01

Voluntary recolonization of sulfide-bearing waste rock dumps by native vegetation is inhibited by the harsh chemical and physical conditions. The success of volunteer vegetation on the waste rock surfaces at the Bingham Canyon (Utah) porphyry copper deposit is most strongly dependent on the soil pH and salinity, and to a lesser extent on physical characteristics such as compaction and distance from seed source. Vegetation cover and richness both decline below a paste pH of about 6 and above a paste conductivity of about 0.7 dS/m (for a 1:1 soil to water mixture). No significant vegetation establishment occurs below a soil pH of about 4.5. Young sulfide-bearing waste rock surfaces at Bingham Canyon have high salinity, but as reactive pyrite is depleted and salts are flushed from the soil, the salinity eventually declines, allowing volunteer native vegetation to become established on surfaces with a circumneutral pH. Under natural conditions, the pH of older acidic weathered surfaces will recover very slowly, but it can be rapidly raised by adding relatively small amounts of limestone because there are few intact reactive sulfides. For uncompacted waste rock surfaces with favorable chemical conditions, less than 90% gravel content, and that are located near a native seed source, the arithmetic mean volunteer vegetation cover was 56 +/- 24% and the mean species richness was 17 +/- 5. These data indicate that with adequate surface preparation and limestone addition, direct planting of older, acidic, but low salinity waste rock surfaces can greatly accelerate natural revegetation.

Anomalous pH-Dependent Nanofluidic Salinity Gradient Power.

PubMed

Yeh, Li-Hsien; Chen, Fu; Chiou, Yu-Ting; Su, Yen-Shao

2017-12-01

Previous studies on nanofluidic salinity gradient power (NSGP), where energy associated with the salinity gradient can be harvested with ion-selective nanopores, all suggest that nanofluidic devices having higher surface charge density should have higher performance, including osmotic power and conversion efficiency. In this manuscript, this viewpoint is challenged and anomalous counterintuitive pH-dependent NSGP behaviors are reported. For example, with equal pH deviation from its isoelectric point (IEP), the nanopore at pH < IEP is shown to have smaller surface charge density but remarkably higher NSGP performance than that at pH > IEP. Moreover, for sufficiently low pH, the NSGP performance decreases with lowering pH (increasing nanopore charge density). As a result, a maximum osmotic power density as high as 5.85 kW m -2 can be generated along with a conversion efficiency of 26.3% achieved for a single alumina nanopore at pH 3.5 under a 1000-fold concentration ratio. Using the rigorous model with considering the surface equilibrium reactions on the pore wall, it is proved that these counterintuitive surface-charge-dependent NSGP behaviors result from the pH-dependent ion concentration polarization effect, which yields the degradation in effective concentration ratio across the nanopore. These findings provide significant insight for the design of next-generation, high-performance NSGP devices. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Graphical coding data and operational guidance for implementation or modification of a LabVIEW®-based pHstat system for the cultivation of microalgae.

PubMed

Golda, Rachel L; Golda, Mark D; Peterson, Tawnya D; Needoba, Joseph A

2017-06-01

The influence of pH on phytoplankton physiology is an important facet of the body of research on ocean acidification. We provide data developed during the design and implementation of a novel pHstat system capable of maintaining both static and dynamic pH environments in a laboratory setting. These data both help improve functionality of the system, and provide specific coding blocks for controlling the pHstat using a LabVIEW® virtual instrument (VI). The data in this paper support the research article "Development of an economical, autonomous pHstat system for culturing phytoplankton under steady state or dynamic conditions" (Golda et al. [2]). These data will be of interest to researchers studying the effects of changing pH on phytoplankton in a laboratory context, and to those desiring to build their own pHstat system(s). These data can also be used to facilitate modification of the pHstat system to control salinity, temperature, or other environmental factors.

Enhanced phosphorus removal from wastewater by growing deep-sea bacterium combined with basic oxygen furnace slag.

PubMed

Zhou, Weizhi; Huang, Zhaosong; Sun, Cuiping; Zhao, Haixia; Zhang, Yuzhong

2016-08-01

As one solid waste with potential for phosphorus removal, application of slags in water treatment merits attention. But it was inhibited greatly by alkaline solution (pH>9.5) and cemented clogging generated. To give one solution, phosphorus removal was investigated by combining deep-sea bacterium Alteromonas 522-1 and basic oxygen furnace slag (BOFS). Results showed that by the combination, not only higher phosphorous removal efficiency (>90%) but also neutral solution pH of 7.8-8.0 were achieved at wide ranges of initial solution pH value of 5.0-9.0, phosphorus concentration of 5-30mg/L, salinity of 0.5-3.5%, and temperature of 15-35°C. Moreover, sedimentary property was also improved with lower amount of sludge production and alleviated BOFS cementation with increased porosity and enlarged particle size. These results provided a promising strategy for the phosphorus recovery with slags in large-scale wastewater treatment. Copyright © 2016 Elsevier Ltd. All rights reserved.

Summertime sea surface temperature fronts associated with upwelling around the Taiwan Bank

NASA Astrophysics Data System (ADS)

Lan, Kuo-Wei; Kawamura, Hiroshi; Lee, Ming-An; Chang, Yi; Chan, Jui-Wen; Liao, Cheng-Hsin

2009-04-01

It is well known that upwelling of subsurface water is dominant around the Taiwan Bank (TB) and the Penghu (PH) Islands in the southern Taiwan Strait in summertime. Sea surface temperature (SST) frontal features and related phenomena around the TB upwelling and the PH upwelling were investigated using long-term AVHRR (1996-2005) and SeaWiFS (1998-2005) data received at the station of National Taiwan Ocean University. SST and chlorophyll-a (Chl-a) images with a spatial resolution of 0.01° were generated and used for the monthly SST and Chl-a maps. SST fronts were extracted from each SST images and gradient magnitudes (GMs); the orientations were derived for the SST fronts. Monthly maps of cold fronts where the cooler SSTs were over a shallower bottom were produced from the orientation. Areas with high GMs (0.1-0.2 °C/km) with characteristic shapes appeared at geographically fixed positions around the TB/PH upwelling region where SSTs were lower than the surrounding waters. The well-shaped high GMs corresponded to cold fronts. Two areas with high Chl-a were found around the TB and PH Islands. The southern border of the high-Chl-a area in the TB upwelling area was outlined by the high-GM area. Shipboard measurements of snapshot vertical sections of temperature (T) and salinity (S) along the PH Channel showed a dome structure east of PH Islands, over which low SST and high GM in the maps of the corresponding month were present. Clear evidence of upwelling (vertically uniform distributions of T and S) was indicated at the TB edge in the T and S sections close to TB upwelling. This case of upwelling may be caused by bottom currents ascending the TB slope as pointed out by previous studies. The position of low SSTs in the monthly maps matched the upwelling area, and the high GMs corresponded to the area of eastern surface fronts in the T/S sections.

Increased temperatures combined with lowered salinities differentially impact oyster size class growth and mortality

USGS Publications Warehouse

LaPeyre, Megan K.; Rybovich, Molly; Hall, Steven G.; La Peyre, Jerome F.

2016-01-01

Changes in the timing and interaction of seasonal high temperatures and low salinities as predicted by climate change models could dramatically alter oyster population dynamics. Little is known explicitly about how low salinity and high temperature combinations affect spat (<25mm), seed (25–75mm), andmarket (>75mm) oyster growth and mortality. Using field and laboratory studies, this project quantified the combined effects of extremely low salinities (<5) and high temperatures (>30°C) on growth and survival of spat, seed, andmarket-sized oysters. In 2012 and 2013, hatchery-produced oysters were placed in open and closed cages at three sites in Breton Sound, LA, along a salinity gradient that typically ranged from 5 to 20. Growth and mortality were recorded monthly. Regardless of size class, oysters at the lowest salinity site (annualmean = 4.8) experienced significantly highermortality and lower growth than oysters located in higher salinity sites (annual means = 11.1 and 13.0, respectively); furthermore, all oysters in open cages at the two higher salinity sites experienced higher mortality than in closed cages, likely due to predation. To explicitly examine oyster responses to extreme low salinity and high temperature combinations, a series of laboratory studies were conducted. Oysters were placed in 18 tanks in a fully crossed temperature (25°C, 32°C) by salinity (1, 5, and 15) study with three replicates, and repeated at least twice for each oyster size class. Regardless of temperature, seed and market oysters held in low salinity tanks (salinity 1) experienced 100% mortality within 7 days. In contrast, at salinity 5, temperature significantly affected mortality; oysters in all size classes experienced greater than 50%mortality at 32°C and less than 40%mortality at 25°C. At the highest salinity tested (15), only market-sized oysters held at 32°C experienced significant mortality (>60%). These studies demonstrate that high water temperatures (>30°C) and low salinities (<5) negatively impact oyster growth and survival differentially and that high temperatures alone may negatively impact market-sized oysters. It is critical to understand the potential impacts of climate and anthropogenic changes on oyster resources to better adapt and manage for long-term sustainability.

Novel levan and pNIPA temperature sensitive hydrogels for 5-ASA controlled release.

PubMed

Osman, Asila; Oner, Ebru Toksoy; Eroglu, Mehmet S

2017-06-01

Levan based cross-linker was successfully synthesized and used to prepare a series of more biocompatible and temperature responsive levan/N-isopropyl acrylamide (levan/pNIPA) hydrogels by redox polymerization at room temperature. Volume phase transition temperature (VPTT) of the hydrogels were precisely determined by derivative differential scanning calorimetry (DDSC). Incorporation of levan into the pNIPA hydrogel increased the VPTT from 32.8°C to 35.09°C, approaching to body temperature. Swelling behavior and 5-aminosalicylic acid (5-ASA) release of the hydrogels were found to vary significantly with temperature and composition. Moreover, a remarkable increase in thermal stability of levan within hydrogel with increase of pNIPA content was recorded. The biocompatibility of the hydrogels were tested against mouse fibroblast L929 cell line in phosphate buffer saline (PBS, pH 7.4). The hydrogels showed increasing biocompatibility with increasing levan ratio, indicating levan enhanced the hydrogel surface during swelling. Copyright © 2017 Elsevier Ltd. All rights reserved.

High Temperature and Salinity Enhance Soil Nitrogen Mineralization in a Tidal Freshwater Marsh

PubMed Central

Gao, Haifeng; Bai, Junhong; He, Xinhua; Zhao, Qingqing; Lu, Qiongqiong; Wang, Junjing

2014-01-01

Soil nitrogen (N) mineralization in wetlands is sensitive to various environmental factors. To compare the effects of salinity and temperature on N mineralization, wetland soils from a tidal freshwater marsh locating in the Yellow River Delta was incubated over a 48-d anaerobic incubation period under four salinity concentrations (0, 10, 20 and 35‰) and four temperature levels (10, 20, 30 and 40°C). The results suggested that accumulated ammonium nitrogen (NH4 +-N) increased with increasing incubation time under all salinity concentrations. Higher temperatures and salinities significantly enhanced soil N mineralization except for a short-term (≈10 days) inhibiting effect found under 35‰ salinity. The incubation time, temperature, salinity and their interactions exhibited significant effects on N mineralization (P<0.001) except the interactive effect of salinity and temperature (P>0.05), while temperature exhibited the greatest effect (P<0.001). Meanwhile, N mineralization processes were simulated using both an effective accumulated temperature model and a one-pool model. Both models fit well with the simulation of soil N mineralization process in the coastal freshwater wetlands under a range of 30 to 40°C (R2 = 0.88–0.99, P<0.01). Our results indicated that an enhanced NH4 +-N release with increasing temperature and salinity deriving from the projected global warming could have profound effects on nutrient cycling in coastal wetland ecosystems. PMID:24733366

High Levels of Sediment Contamination Have Little Influence on Estuarine Beach Fish Communities

PubMed Central

McKinley, Andrew C.; Dafforn, Katherine A.; Taylor, Matthew D.; Johnston, Emma L.

2011-01-01

While contaminants are predicted to have measurable impacts on fish assemblages, studies have rarely assessed this potential in the context of natural variability in physico-chemical conditions within and between estuaries. We investigated links between the distribution of sediment contamination (metals and PAHs), physico-chemical variables (pH, salinity, temperature, turbidity) and beach fish assemblages in estuarine environments. Fish communities were sampled using a beach seine within the inner and outer zones of six estuaries that were either heavily modified or relatively unmodified by urbanization and industrial activity. All sampling was replicated over two years with two periods sampled each year. Shannon diversity, biomass and abundance were all significantly higher in the inner zone of estuaries while fish were larger on average in the outer zone. Strong differences in community composition were also detected between the inner and outer zones. Few differences were detected between fish assemblages in heavily modified versus relatively unmodified estuaries despite high concentrations of sediment contaminants in the inner zones of modified estuaries that exceeded recognized sediment quality guidelines. Trends in species distributions, community composition, abundance, Shannon diversity, and average fish weight were strongly correlated to physico-chemical variables and showed a weaker relationship to sediment metal contamination. Sediment PAH concentrations were not significantly related to the fish assemblage. These findings suggest that variation in some physico-chemical factors (salinity, temperature, pH) or variables that co-vary with these factors (e.g., wave activity or grain size) have a much greater influence on this fish assemblage than anthropogenic stressors such as contamination. PMID:22039470

The effect of climate change on the distribution of a tropical zoanthid (Palythoa caribaeorum) and its ecological implications.

PubMed

Durante, Leonardo M; Cruz, Igor C S; Lotufo, Tito M C

2018-01-01

Palythoa caribaeorum is a zoanthid often dominant in shallow rocky environments along the west coast of the Atlantic Ocean, from the tropics to the subtropics. This species has high environmental tolerance and is a good space competitor in reef environments. Considering current and future scenarios in the global climate regime, this study aimed to model and analyze the distribution of P. caribaeorum , generating maps of potential distribution for the present and the year 2100. The distribution was modeled using maximum entropy (Maxent) based on 327 occurrence sites retrieved from the literature. Calcite concentration, maximum chlorophyll- a concentration, salinity, pH, and temperature range yielded a model with the smallest Akaike information criterion (2649.8), and were used in the present and future distribution model. Data from the HadGEM2-ES climate model were used to generate the projections for the year 2100. The present distribution of P. caribaeorum shows that parts of the Brazilian coast, Caribbean Sea, and Florida are suitable regions for the species, as they are characterized by high salinity and pH and small temperature variation. An expansion of the species' distribution was forecast northward under mild climate scenarios, while a decrease of suitable areas was forecast in the south. In the climate scenario with the most intense changes, P. caribaeorum would lose one-half of its suitable habitats, including the northernmost and southernmost areas of its distribution. The Caribbean Sea and northeastern Brazil, as well as other places under the influence of coastal upwellings, may serve as potential havens for this species.

The effect of climate change on the distribution of a tropical zoanthid (Palythoa caribaeorum) and its ecological implications

PubMed Central

Cruz, Igor C.S.

2018-01-01

Palythoa caribaeorum is a zoanthid often dominant in shallow rocky environments along the west coast of the Atlantic Ocean, from the tropics to the subtropics. This species has high environmental tolerance and is a good space competitor in reef environments. Considering current and future scenarios in the global climate regime, this study aimed to model and analyze the distribution of P. caribaeorum, generating maps of potential distribution for the present and the year 2100. The distribution was modeled using maximum entropy (Maxent) based on 327 occurrence sites retrieved from the literature. Calcite concentration, maximum chlorophyll-a concentration, salinity, pH, and temperature range yielded a model with the smallest Akaike information criterion (2649.8), and were used in the present and future distribution model. Data from the HadGEM2-ES climate model were used to generate the projections for the year 2100. The present distribution of P. caribaeorum shows that parts of the Brazilian coast, Caribbean Sea, and Florida are suitable regions for the species, as they are characterized by high salinity and pH and small temperature variation. An expansion of the species’ distribution was forecast northward under mild climate scenarios, while a decrease of suitable areas was forecast in the south. In the climate scenario with the most intense changes, P. caribaeorum would lose one-half of its suitable habitats, including the northernmost and southernmost areas of its distribution. The Caribbean Sea and northeastern Brazil, as well as other places under the influence of coastal upwellings, may serve as potential havens for this species. PMID:29785350

Furfural and its biochar improve the general properties of a saline soil

NASA Astrophysics Data System (ADS)

Wu, Y.; Xu, G.; Shao, H. B.

2014-07-01

Organic materials (e.g., furfural residue) are generally believed to improve the physical and chemical properties of saline soils with low fertility. Recently, biochar has been received more attention as a possible measure to improve the carbon balance and improve soil quality in some degraded soils. However, little is known about their different amelioration of a sandy saline soil. In this study, 56 d incubation experiment was conducted to evaluate the influence of furfural and its biochar on the properties of saline soil. The results showed that both furfural and biochar greatly reduced pH, increased soil organic carbon (SOC) content and cation exchange capacity (CEC), and enhanced the available phosphorus (P) in the soil. Furfural is more efficient than biochar in reducing pH: 5% furfural lowered the soil pH by 0.5-0.8 (soil pH: 8.3-8.6), while 5% biochar decreased by 0.25-0.4 due to the loss of acidity in pyrolysis process. With respect to available P, furfural addition at a rate of 5% increased available P content by 4-6 times in comparison to 2-5 times with biochar application. In reducing soil exchangeable sodium percentage (ESP), biochar is slightly superior to furfural because soil ESP reduced by 51% and 43% with 5% furfural and 5% biochar at the end of incubation. In addition, no significant differences were observed between furfural and biochar about their capacity to retain N, P in leaching solution and to increase CEC in soil. These facts may be caused by the relatively short incubation time. In general, furfural and biochar exhibited a different effect depending on the property: furfural was more effective in decreasing pH and increasing available P, whereas biochar played a more important role in increasing SOC and reducing ESP of saline soil.

Ammonium stability and nitrogen isotope fractionations for NH4+-NH3(aq)-NH3(gas) systems at 20-70 °C and pH of 2-13: Applications to habitability and nitrogen cycling in low-temperature hydrothermal systems

NASA Astrophysics Data System (ADS)

Li, Long; Lollar, Barbara Sherwood; Li, Hong; Wortmann, Ulrich G.; Lacrampe-Couloume, Georges

2012-05-01

Ammonium/ammonia is an essential nutrient and energy source to support life in oceanic and terrestrial hydrothermal systems. Thus the stability of ammonium is crucial to determine the habitability or ecological structure in hydrothermal environments, but still not well understood. To date, the lack of constraints on nitrogen isotope fractionations between ammonium and ammonia has limited the application of nitrogen isotopes to trace (bio)geochemical processes in such environments. In this study, we carried out laboratory experiments to (1) examine the stability of ammonium in an ammonium sulfate solution under temperature conditions from 20 to 70 °C and pH from 2.1 to 12.6 and (2) determine nitrogen isotope fractionation between ammonium and ammonia. Our experimental results show that ammonium is stable under the experimental temperatures when pH is less than 6. In experiments with starting pH greater than 8, significant ammonium was lost as a result of dissociation of ammonium and degassing of ammonia product. Nitrogen concentrations in the fluids decreased by more than 50% in the first two hours, indicating extremely fast effusion rates of ammonia. This implies that ammonium at high pH fluids (e.g., Lost City Hydrothermal Vents, Oman ophiolite hyperalkaline springs) may not be stable. Habitable environments may be more favorable at the leading edge of a pH gradient toward more acidic conditions, where the fluid can efficiently trap any ammonia transferred from a high pH vent. Although modeling shows that high temperature, low pH hydrothermal vents (e.g., Rainbow hydrothermal vent) may have the capability to retain ammonium, their high temperatures may limit habitability. The habitable zone associated with such a hydrothermal vent is likely at the lower front of a temperature gradient. In contrast, modeling of ammonium in deep terrestrial systems, suggests that saline fracture waters in crystalline rocks such as described in the Canadian Shield and in the Witwatersrand Basin, South Africa may also provide habitable environments for life. The nitrogen isotope results of remaining ammonium from the partial dissociation experiments fit well with a batch equilibrium model, indicating equilibrium nitrogen isotope fractionations have been reached between ammonium and its dissociation product aqueous ammonia. Modeling yielded nitrogen isotope fractionations between ammonium and aqueous ammonia were 45.4‰ at 23 °C, 37.7‰ at 50 °C, and 33.5‰ at 70 °C, respectively. A relationship between nitrogen equilibrium isotope fractionation and temperature is determined for the experimental temperature range as: 103·lnα(aq)=25.94×{103}/{T}-42.25 Integrated with three previous theoretical estimates on nitrogen isotope equilibrium fractionations between ammonium and gaseous ammonia, we achieved three possible temperature-dependent nitrogen isotope equilibrium fractionation between aqueous ammonia and gaseous ammonia:

Studies on the Time Course of Salinity and Temperature Adaptation in the Commercial Brown Shrimp Penaeus aztecus Ives.

DTIC Science & Technology

1977-09-01

Effect of temperature background 52 Effect of salinity and temperature change 53 Blood Osmoregulation During the Time Course of Adaptation... Osmoregulators Osmotic concentration Salinity Serum Standard metabolism Weight specific metabolic rate APPENDIX B: Tables I-IX. Mean... Effect of salinity and temperature change on the blood osmoregulation of Penaeus aztecus in relation to the isosmotic line 153 81

Using Foraminifera Shell Geochemistry to Test Proxies for Paleoclimate Reconstruction

NASA Astrophysics Data System (ADS)

Hudson, C. Y.; Herrmann, A. D.

2016-02-01

Previous research conducted on Foraminifera tests determined that the elemental concentrations of the shell reflect pH and salinity of the environment these organisms lived in. Changes in concentrations of Boron and Uranium were analyzed because they are both pH and salinity indicators. For this project, sand samples were gathered from two different stations along Terrebonne Bay in the Gulf of Mexico. Each station had a different depth and salinity. Alabaminella sp. were then separated from the sand sample. Foraminifera were analyzed using an LA-ICP-MS to determine if there were geochemical differences in the shells from different stations. If differences were determined to exist, then it would be possible to use these differences to reconstruct changes in past coastal environments. Because the depth and salinity at each Terrebonne station was different, it was expected that the geochemisty of the shells would also be different. It has been concluded that the Foraminifera shells from each station show no significant variation in their Boron and Uranium concentrations. Therefore, paleoclimate pH and salinity cannot be accurately reconstructed using these Foraminifera collected from Terrebonne Bay. The similarity of the geochemistries is likely due to the short distance between stations, allowing the Foraminifera to easily move from one location to another. Further tests can evaluate whether Foraminifera collected from stations farther apart do show the expected geochemical changes that result from different environments.

Influence of DOM components, salinity, pH, nitrate, and bicarbonate on the indirect photodegradation of acetaminophen in simulated coastal waters.

PubMed

Bai, Ying; Cui, Zhengguo; Su, Rongguo; Qu, Keming

2018-04-18

The indirect photodegradation behaviors of acetaminophen (APAP) were investigated in the presence of four kinds of dissolved organic matter (DOM) and were also assessed in the presence of seawater components and conditions such as salinity, pH, nitrate and bicarbonate. The results showed three important findings: firstly, in the indirect photolysis of APAP, the contributions of 3 DOM*, ·OH and 1 O 2 were >85.0%, 2.3-9.9% and 0.8-2.6% at pH 8.0. Secondly, DOM was divided into four terrestrial humic-like components by Excitation-emission matrix spectroscopy (EEMs) combined with parallel factor analysis (PARAFAC). This study showed a good linearity between DOM fluorescence components and the indirect photodegradation of APAP (R 2  = 0.92) and the differences in photodegradation rates of APAP among various DOM solutions were due to the diverse compositions of DOM. Finally, salinity was an important factor influencing the removal of APAP, and the APAP photodegradation rate constants increased from (3.33 ± 0.07) × 10 -5 s -1 to (1.25 ± 0.05) × 10 -4 s -1 with increasing salinity. The increased pseudo-first-order rate constants for photolysis of APAP with increasing salinity, pH and nitrate were attributed to the enhanced generation of reactive intermediates (RI) and easier reactions between RI and APAP. The increased APAP removal rate constant with increasing bicarbonate was likely ascribed to the yield of ∙CO 3 - . This is the first report of the roles of DOM components and salinity on the indirect photolysis of APAP. These findings would be essential to predict the photochemical fate of APAP and would also allow for a better understanding of the environmental fate of other phenolic contaminants. Copyright © 2018 Elsevier Ltd. All rights reserved.

Dunaliella spp. Under Environmental Stress: Enhancing Lipid Production and Optimizing Harvest

NASA Astrophysics Data System (ADS)

Mixson, Stephanie Marie

Agricultural crops including corn, sugar cane, and oil palm have been investigated as potential sources for biofuel; however, they produce only a fraction of the oil percent biomass as compared to that of microalgae. Growth and lipid production by microalgae is regulated by a variety of environmental factors, including light intensity, availability of nutrients, temperature regime and salinity. We assessed 14 strains of the saltwater algae Dunaliella spp. (Teodoresco) in unialgal cultures within four species to determine a best strain or strain(s) as potential feedstock for biofuels. The taxonomy of these 14 strains was elucidated by comparing both physiological characteristics and the ITS2 and 18S regions. After careful analysis, the data suggest that the 14 strains grouped within four species: D. tertiolecta, D. pseudosalina, D. salina, and D. viridis. In addition, the isolation and accurate quantification of neutral lipids in Dunaliella was developed from existing techniques. Nile Red was optimized as a qualitative stain to rapidly screen and visualize neutral lipids. Direct transesterification was determined to be the best quantitative method because it yielded high amounts of neutral lipids with precise and reproducible results when compared to conventional extraction methods. Seven strains were selected for further efforts to enhance lipid production using salinity stress, nutrient limitation, pH stress, continuous light, and bubbling with carbon dioxide (CO2). High salinity yielded the maximum total fatty acid (FA) content (up to 65% by dry weight) in comparison to controls (˜10-25% total FAs). High pH x low salinity, low pH, and continuous light x CO2 yielded near maximum FA content (56%, 43%, and 42%, respectively). Nitrogen and/or phosphorus limitation and 12:12 (light:dark photoperiod) x CO 2 did not significantly enhance FA production (23% and 31%, respectively). Results were strain-specific with high intraspecific variation observed within each environmental stressor. Glycerol production, a known mechanism of osmoregulation in Dunaliella, was measured in a short-term salinity stress experiment and found to significantly increase 30 min to 24 hr after exposure. In addition, the glycerol biosynthesis gene, glycerol-3-phosphate dehydrogenase or GPDH, was significantly expressed 30 min to 2 hr in response to hyperosmotic stress. The data suggest that Dunaliella strains may incorporate a proportion of glycerol as triacylglycerol (TAG) under short-term, high-salinity stress. High lipid-producing strains were grown in mass culture, but at this time the commercialization of harvesting has not been proven economically feasible. Autoflocculation, electro-flocculation, and hollow-fiber filtration were compared as potential harvesting mechanisms for the mass culture of Dunaliella spp. Hollow-fiber filtration (>99% biomass recovery) as harvesting mechanism offers many attractive advantages (i.e. reuse of filtrate as culture medium) when compared to auto-flocculation and indirect electroflocculation (>95% biomass recovery). This research provides evidence that Dunaliella can be used as a source of biofuel because these strains can be mass-cultured; their lipids enhanced through a simple high-salinity adjustment; and commercially harvested.

Effect of elevated pCO2 on metabolic responses of porcelain crab (Petrolisthes cinctipes) Larvae exposed to subsequent salinity stress.

PubMed

Miller, Seth H; Zarate, Sonia; Smith, Edmund H; Gaylord, Brian; Hosfelt, Jessica D; Hill, Tessa M

2014-01-01

Future climate change is predicted to alter the physical characteristics of oceans and estuaries, including pH, temperature, oxygen, and salinity. Investigating how species react to the influence of such multiple stressors is crucial for assessing how future environmental change will alter marine ecosystems. The timing of multiple stressors can also be important, since in some cases stressors arise simultaneously, while in others they occur in rapid succession. In this study, we investigated the effects of elevated pCO2 on oxygen consumption by larvae of the intertidal porcelain crab Petrolisthes cinctipes when exposed to subsequent salinity stress. Such an exposure mimics how larvae under future acidified conditions will likely experience sudden runoff events such as those that occur seasonally along portions of the west coast of the U.S. and in other temperate systems, or how larvae encounter hypersaline waters when crossing density gradients via directed swimming. We raised larvae in the laboratory under ambient and predicted future pCO2 levels (385 and 1000 µatm) for 10 days, and then moved them to seawater at ambient pCO2 but with decreased, ambient, or elevated salinity, to monitor their respiration. While larvae raised under elevated pCO2 or exposed to stressful salinity conditions alone did not exhibit higher respiration rates than larvae held in ambient conditions, larvae exposed to elevated pCO2 followed by stressful salinity conditions consumed more oxygen. These results show that even when multiple stressors act sequentially rather than simultaneously, they can retain their capacity to detrimentally affect organisms.

Effect of Elevated pCO2 on Metabolic Responses of Porcelain Crab (Petrolisthes cinctipes) Larvae Exposed to Subsequent Salinity Stress

PubMed Central

Miller, Seth H.; Zarate, Sonia; Smith, Edmund H.; Gaylord, Brian; Hosfelt, Jessica D.; Hill, Tessa M.

2014-01-01

Future climate change is predicted to alter the physical characteristics of oceans and estuaries, including pH, temperature, oxygen, and salinity. Investigating how species react to the influence of such multiple stressors is crucial for assessing how future environmental change will alter marine ecosystems. The timing of multiple stressors can also be important, since in some cases stressors arise simultaneously, while in others they occur in rapid succession. In this study, we investigated the effects of elevated pCO2 on oxygen consumption by larvae of the intertidal porcelain crab Petrolisthes cinctipes when exposed to subsequent salinity stress. Such an exposure mimics how larvae under future acidified conditions will likely experience sudden runoff events such as those that occur seasonally along portions of the west coast of the U.S. and in other temperate systems, or how larvae encounter hypersaline waters when crossing density gradients via directed swimming. We raised larvae in the laboratory under ambient and predicted future pCO2 levels (385 and 1000 µatm) for 10 days, and then moved them to seawater at ambient pCO2 but with decreased, ambient, or elevated salinity, to monitor their respiration. While larvae raised under elevated pCO2 or exposed to stressful salinity conditions alone did not exhibit higher respiration rates than larvae held in ambient conditions, larvae exposed to elevated pCO2 followed by stressful salinity conditions consumed more oxygen. These results show that even when multiple stressors act sequentially rather than simultaneously, they can retain their capacity to detrimentally affect organisms. PMID:25295878

Sensitivity of Clay Suspension Rheological Properties to pH, Temperature, Salinity, and Smectite-Quartz Ratio

NASA Astrophysics Data System (ADS)

Kameda, Jun; Morisaki, Tomonori

2017-10-01

Understanding the rheological properties of clay suspensions is critical to assessing the behavior of sediment gravity flows such as debris flow or turbidity current. We conducted rheological measurements of composite smectite-quartz suspensions at a temperature of 7°C and a salt concentration of 0.6 M. This is representative of smectite-bearing sediments under conditions on the seafloor. The flow curves obtained were fitted by the Bingham fluid model, from which we determined the Bingham yield stress and dynamic viscosity of each suspension. At a constant smectite-quartz mixing ratio, the yield stress and the dynamic viscosity tend to increase as the solid/water ratio of the suspension is increased. In the case of a constant solid/water ratio, these values increase with increasing smectite content in the smectite-quartz mixture. Additional experiments exploring differing physicochemical conditions (pH 1.0-9.0; temperature 2-30°C; and electrolyte (NaCl) concentration 0.2-0.6 M) revealed that the influence of temperature is negligible, while pH moderately affects the rheology of the suspension. More significantly, the electrolyte concentration greatly affects the flow behavior. These variations can be explained by direct and/or indirect (double-layer) interactions between smectite-smectite particles as well as between smectite-quartz particles in the suspension. Although smectite is known as a frictionally weak material, our experimental results suggest that its occurrence can reduce the likelihood that slope failure initiates. Furthermore, smectite can effectively suppress the spreading distance once the slope has failed.

Adsorption of organic ligands on low surface charge clay minerals: the composition in the aqueous interface region.

PubMed

Jelavić, S; Stipp, S L S; Bovet, N

2018-06-27

An understanding of the mechanisms that control the adsorption of organic molecules on clay minerals is of interest in several branches of science and industry. Oil production using low salinity injection fluids can increase yields by as much as 40% over standard injection with seawater or formation water. The mechanism responsible for the low salinity response is still debated, but one hypothesis is a change in pore surface wettability. Organic contamination in soil and drinking water aquifers is a challenge for municipal water suppliers and for agriculture. A better understanding is needed for how mineral species, solution composition and pH affect the desorption of low molecular weight organic ligands from clay minerals and consequently their wettability. We used X-ray photoelectron spectroscopy under cryogenic conditions to investigate the in situ composition in the mineral-solution interface region in a series of experiments with a range of pH and ion concentrations. We demonstrate that both chlorite and kaolinite release organic molecules under conditions relevant for low salinity water flooding. This release increases with a higher solution pH but is only slightly affected by the character of the organic ligand. This is consistent with the observation that low salinity enhanced oil recovery correlates with the presence of chlorite and kaolinite. Our results indicate that the pore surface charge and salinity of formation water and injection fluids are key parameters in determining the low salinity response. In general, our results imply that clay mineral surface charge influences the composition in the interface through an affinity for organic molecules.

Musculoskeletal sensitization and sleep: chronic muscle pain fragments sleep of mice without altering its duration.

PubMed

Sutton, Blair C; Opp, Mark R

2014-03-01

Musculoskeletal pain in humans is often associated with poor sleep quality. We used a model in which mechanical hypersensitivity was induced by injection of acidified saline into muscle to study the impact of musculoskeletal sensitization on sleep of mice. A one month pre-clinical study was designed to determine the impact of musculoskeletal sensitization on sleep of C57BL/6J mice. We instrumented mice with telemeters to record the electroencephalogram (EEG) and body temperature. We used an established model of musculoskeletal sensitization in which mechanical hypersensitivity was induced using two unilateral injections of acidified saline (pH 4.0). The injections were given into the gastrocnemius muscle and spaced five days apart. EEG and body temperature recordings started prior to injections (baseline) and continued for three weeks after musculoskeletal sensitization was induced by the second injection. Mechanical hypersensitivity was assessed using von Frey filaments at baseline (before any injections) and on days 1, 3, 7, 14, and 21 after the second injection. Mice injected with acidified saline developed bilateral mechanical hypersensitivity at the hind paws as measured by von Frey testing and as compared to control mice and baseline data. Sleep during the light period was fragmented in experimental mice injected with acidified saline, and EEG spectra altered. Musculoskeletal sensitization did not alter the duration of time spent in wakefulness, non-rapid eye movement sleep, or rapid eye movement sleep. Musculoskeletal sensitization in this model results in a distinct sleep phenotype in which sleep is fragmented during the light period, but the overall duration of sleep is not changed. This study suggests the consequences of musculoskeletal pain include sleep disruption, an observation that has been made in the clinical literature but has yet to be studied using preclinical models.

Synergistic Action of a Microbial-based Biostimulant and a Plant Derived-Protein Hydrolysate Enhances Lettuce Tolerance to Alkalinity and Salinity

PubMed Central

Rouphael, Youssef; Cardarelli, Mariateresa; Bonini, Paolo; Colla, Giuseppe

2017-01-01

In the coming years, farmers will have to deal with growing crops under suboptimal conditions dictated by global climate changes. The application of plant biostimulants such as beneficial microorganisms and plant-derived protein hydrolysates (PHs) may represent an interesting approach for increasing crop tolerance to alkalinity and salinity. The current research aimed at elucidating the agronomical, physiological, and biochemical effects as well as the changes in mineral composition of greenhouse lettuce (Lactuca sativa L.) either untreated or treated with a microbial-based biostimulant (Tablet) containing Rhizophagus intraradices and Trichoderma atroviride alone or in combination with a PH. Plants were sprayed with PH at weekly intervals with a solution containing 2.5 ml L-1 of PH. Lettuce plants were grown in sand culture and supplied with three nutrient solutions: standard, saline (25 mM NaCl) or alkaline (10 mM NaHCO3 + 0.5 g l-1 CaCO3; pH 8.1). Salt stress triggered a decrease in fresh yield, biomass production, SPAD index, chlorophyll fluorescence, leaf mineral composition and increased leaf proline concentration, without altering antioxidant enzyme activities. The decrease in marketable yield and biomass production under alkali stress was not significant. Irrespective of nutrient solution, the application of Tablet and especially Tablet + PH increased fresh marketable yield, shoot and root dry weight. This was associated with an improvement in SPAD index, Fv/Fm ratio, CAT and GPX activities and a better nutritional status (higher P, K, and Fe and lower Na with NaCl and higher P and Fe with NaHCO3) via an increase of total root length and surface. The combination of microbial biostimulant with foliar application of PH synergistically increased the marketable fresh yield by 15.5 and 46.7% compared to the Tablet-treated and untreated plants, respectively. The improved crop performance of Tablet + PH application was attributed to a better root system architecture (higher total root length and surface), an improved chlorophyll synthesis and an increase in proline accumulation. Combined application of Tablet and PH could represent an effective strategy to minimize alkalinity and salinity stress in a sustainable way. PMID:28223995

Linking water and carbon cycles through salinity observed from space

NASA Astrophysics Data System (ADS)

Xie, X.; Liu, W. T.

2017-12-01

The association of ocean surface salinity in global hydrological cycle and climate change has been traditionally studied through the examination of its tendency and advection as manifestation of ocean's heat and water fluxes with the atmosphere. The variability of surface heat and water fluxes are linked to top of atmosphere radiation, whose imbalance is the main cause of global warming. Besides the link of salinity to greenhouse warming through water balance, this study will focus on the effect of changing salinity on carbon dioxide flux between the ocean and the atmosphere. We have built statistical models to estimate the partial pressure of carbon dioxide (pCO2) and ocean acidification (in terms of total alkalinity and pH) using spacebased data. PCO2 is a critical parameter governing ocean as source and sink of the accumulated greenhouse gas in the atmosphere. The exchange also causes ocean acidification, which is detrimental to marine lives and ecology. Before we had sufficient spacebased salinity measurements coincident with in situ pCO2 measurement, we trained our statistical models to use satellite sea surface temperature and chlorophyll, with one model using salinity climatology and the other without. We found significant differences between the two models in regions of strong water input through river discharge and surface water flux. The pCO2 output follows the seasonal salinity advection of the Amazon outflow. The seasonal salinity advection between Bay of Bengal and Arabian Sea are followed by change of pCO2 and total alkalinity. At shorter time scales, the signatures of rain associated with intraseasonal organized convection of summer monsoon can be detected. We have observed distribution agreement of among pCO2, surface salinity, and surface water flux for variation from a few days to a few years under the Pacific ITCZ; the agreement varies slightly with season and longitudes and the reason is under study.

Hydrothermal mobilization of pegmatite-hosted REE and Zr at Strange Lake, Canada: A reaction path model

NASA Astrophysics Data System (ADS)

Gysi, Alexander P.; Williams-Jones, Anthony E.

2013-12-01

Petrological and geochemical observations of pegmatites in the Strange Lake pluton, Canada, have been combined with numerical simulations to improve our understanding of fluid-rock interaction in peralkaline granitic systems. In particular, they have made it possible to evaluate reaction paths responsible for hydrothermal mobilization and mineralization of rare earth elements (REE) and Zr. The focus of the study was the B-Zone in the northwest of the pluton, which contains a pegmatite swarm and is the target of exploration for an economically exploitable REE deposit. Many of the pegmatites are mineralogically zoned into a border consisting of variably altered primary K-feldspar, arfvedsonite, quartz, and zirconosilicates, and a core rich in quartz, fluorite and exotic REE minerals. Textural relationships indicate that the primary silicate minerals in the pegmatites were leached and/or replaced during acidic alteration by K-, Fe- and Al-phyllosilicates, aegirine, hematite, fluorite and/or quartz, and that primary zirconosilicates (e.g., elpidite) were replaced by gittinsite and/or zircon. Reaction textures recording coupled dissolution of silicate minerals and crystallization of secondary REE-silicates indicate hydrothermal mobilization of the REE. The mobility of the light (L)REE was limited by the stability of REE-F-(CO2)-minerals (basnäsite-(Ce) and fluocerite-(Ce)), whereas zirconosilicates and secondary gadolinite-group minerals controlled the mobility of Zr and the heavy (H)REE. Hydrothermal fluorite and fluorite-fluocerite-(Ce) solid solutions are interpreted to indicate the former presence of F-bearing saline fluids in the pegmatites. Numerical simulations show that the mobilization of REE and Zr in saline HCl-HF-bearing fluids is controlled by pH, ligand activity and temperature. Mobilization of Zr is significant in both saline HF- and HCl-HF-bearing fluids at low temperature (250 °C). In contrast, the REE are mobilized by saline HCl-bearing fluids, particularly at high temperature (400 °C). The LREE are more mobile than the HREE in saline HCl-bearing fluids due to the greater stability of LREE-chloride complexes. The simulated mineralogy is consistent with the zonation observed in the pegmatites and with fluid-rock interaction at conditions that were rock-buffered in the pegmatite borders (low fluid/rock ratio; and pH > 4) and fluid-buffered in the cores (high fluid/rock ratio; pH ⩽ 2). We propose a model in which saline HCl-HF-bearing fluids created pathways during acidic alteration from the pegmatite cores outward. This led to the mobilization of REE and Zr due to progressive alteration of primary silicate minerals and increased acidity upon cooling. The key requirement for REE and Zr mobilization in peralkaline igneous intrusions is the formation of an acidic subsystem with high fluid/rock ratios that increases the overall permeability of the rocks. In these zones, the extent of late stage hydrothermal redistribution and concentration of REE and Zr depends on the buffering capacity of the rocks and the availability of fluids that may produce autometasomatic rock alteration, interact with external rock units and/or mix with fluids from other sources. b The detection limits of Yb were 1043 ppm for zircon, 380 ppm for gadolinite-group minerals and 380 ppm for REE-F-(CO2)-minerals. bAja et al. (1995). cMigdisov et al. (2011). dTagirov et al. (1997). eTagirov and Schott (2001). fMigdisov et al. (2009) with REE (La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu). b Calculated using the methods of the Chermak and Rimstidt (1989), Berman and Brown (1985) and Holland (1989) with molar volume of arfvedsonite from Hawthorne (1976). cZotov et al. (1998). d GEM-Selektor v.3 database (http://gems.web.psi.ch). eMigdisov et al. (2009).

Physical characteristics of commercial and home-made nasal lavage solutions.

PubMed

Lilic, N; Waldvogel-Thurlow, S; Douglas, R G

2014-01-01

Nasal saline lavage forms a cornerstone of chronic rhinosinusitis management. A number of saline lavage recipes and products are currently available but little is known of their relative physical characteristics. To determine the osmolarity and pH of nasal lavage solutions, both commercial preparations and home-made recipes, and to determine the reproducibility of saline solution preparation. Five home-made recipes and two commercial products were prepared in triplicate and analysed. Eight consecutive clinic patients prepared a commercial product and 11 prepared a home-made recipe, and the osmolarity and pH were measured. The osmolarity of the solutions varied widely, from 140 to 788 mmol/l and the pH varied from 7.88 to 8.50. The commercial and home-made solutions had similar reproducibility when prepared by patients. Some recipes were markedly hypo-osmolar and some were hyper-osmolar. All were slightly alkaline. The home-made recipes had similar physical characteristics and reproducibility to commercial preparations.

Purification and characterization of haloalkaline thermoactive, solvent stable and SDS-induced protease from Bacillus sp.: a potential additive for laundry detergents.

PubMed

Jain, Deepti; Pancha, Imran; Mishra, Sanjiv K; Shrivastav, Anupama; Mishra, Sandhya

2012-07-01

An extracellular haloalkaline, thermoactive, solvent stable, SDS-induced serine protease was purified and characterized from an alkali-thermo tolerant strain Bacillus sp. SM2014 isolated from reverse osmosis reject. The enzyme was purified to homogeneity with recovery of 54.4% and purity fold of 64. The purified enzyme was composed of single polypeptide of molecular mass about 71 kDa. The enzyme showed optimum activity at alkaline pH 10 and temperature 60°C. The km and Vmax for the enzyme was 0.57 mg/ml and 445.23 U/ml respectively. The enzyme showed novel catalytic ability at high pH (10), temperature (60°C) and salinity (3M). Moreover, the stability of enzyme in organic solvents (50% v/v) of logP ≥ 2 signified the prospective of this enzyme for peptide synthesis. The compatibility of the enzyme with surfactants and various detergent matrices together with wash performance test confirmed its potential applicability in laundry industry. Copyright © 2011 Elsevier Ltd. All rights reserved.

Pearl Harbor Biological Survey

DTIC Science & Technology

1974-08-30

properties, uses, and driving mechanisms affecting the harbor is given. The methods of obtaining current data, salinity profiles, and temperature... salinities were used for each calibration In order to check the salinity computation mechanism of the Instrument. Temperature calibrations were...Water Temperature Contours for Navy Thermal Discharges 3.2-23 3.2-7. General Layout of Pearl Harbor Showing Mean Monthly Salinity (3L) Variation

Random Forest Segregation of Drug Responses May Define Regions of Biological Significance.

PubMed

Bukhari, Qasim; Borsook, David; Rudin, Markus; Becerra, Lino

2016-01-01

The ability to assess brain responses in unsupervised manner based on fMRI measure has remained a challenge. Here we have applied the Random Forest (RF) method to detect differences in the pharmacological MRI (phMRI) response in rats to treatment with an analgesic drug (buprenorphine) as compared to control (saline). Three groups of animals were studied: two groups treated with different doses of the opioid buprenorphine, low (LD), and high dose (HD), and one receiving saline. PhMRI responses were evaluated in 45 brain regions and RF analysis was applied to allocate rats to the individual treatment groups. RF analysis was able to identify drug effects based on differential phMRI responses in the hippocampus, amygdala, nucleus accumbens, superior colliculus, and the lateral and posterior thalamus for drug vs. saline. These structures have high levels of mu opioid receptors. In addition these regions are involved in aversive signaling, which is inhibited by mu opioids. The results demonstrate that buprenorphine mediated phMRI responses comprise characteristic features that allow a supervised differentiation from placebo treated rats as well as the proper allocation to the respective drug dose group using the RF method, a method that has been successfully applied in clinical studies.

Improved understanding of tributyltin sorption on natural and biochar-amended sediments.

PubMed

Xiao, Xiaoyu; Sheng, G Daniel; Qiu, Yuping

2011-12-01

A poor understanding of tributyltin (TBT) sorption on sediments has hindered an accurate evaluation of its environmental fate. The present study determined TBT sorption by a freshwater sediment (BH) and a coastal marine sediment (TZ) as influenced by pH, salinity, and biochar (BC) amendment into TZ. The isotherms were essentially linear, with K(OC) values in the range of 10(4) to 10(5) L/kg. Tributyltin sorption at pH 3.56 and 8.00 occurred mainly via partitioning. It reached maxima at pH equal to its pK(a) (=6.25) because of added ion exchange. A salinity increase from 5 to 35 practical salinity units enhanced TBT sorption at pH 3.56 and 8.00 on TZ by approximately 30% and on BH by approximately 80%, ascribed to the salting-out effect that reduced the solubilities of tributyltin hydroxide (TBTOH) and tributyltin chloride (TBTCl). At pH 6.25, the same salinity increase reduced TBT sorption on TZ by approximately 20% but enhanced TBT sorption on BH by approximately 35%. This was attributed to the enhancing role of salting out and the reducing role of metal competition for ion exchange. Tributyltin was two orders of magnitude more effectively sorbed by BC than by total organic carbon of TZ, mainly because of the high level of surface area of the BC. Although BC affinity for TBT may be significantly diminished when present in TZ, it was considered to be the primary contributor to TBT sorption from water. Biochar may thus be used to immobilize TBT in sediment for potential remediation. Copyright © 2011 SETAC.

The effect of retreatment procedure on the pH changes at the surface of root dentin using two different calcium hydroxide pastes

PubMed Central

Kazemipoor, Maryam; Tabrizizadeh, Mehdi; Dastani, Milad; Hakimian, Roqayeh

2012-01-01

Aim: To compare pH changes at the cervical, middle and apical surfaces of root dentin in retreated and non- retreated teeth, after canal obturation with two different calcium hydroxide pastes. Materials and Methods: After instrumentation of 55 extracted teeth, three cavities with 0.75 mm depth and 1.5 mm in diameter were drilled at buccal root surface. The teeth were randomly divided into five groups. Canals in the first two groups were filled with either mixture of calcium hydroxide and saline solution and calcium hydroxide and 2% chlorhexidine (CHX). In the third and fourth groups canals were first obturated with gutta-percha and AH26 sealer, and then materials were removed. After 2 days canals were filled with two different calcium hydroxide pastes similar to the first and the second groups. The pH was measured in the prepared cavities at 1, 3, 7 and 14 days. Results: In the non-retreated groups, pH at the surface of the roots was significantly higher in comparison to the retreated ones (P value < 0.001). pH values were significantly higher in the non-retreated teeth filling with calcium hydroxide and saline solution (P value < 0.001). Conclusion: Regarding to the little pH changes at the surface of dentin in retreated teeth, the hydroxyl ions cannot penetrate into the dentinal tubules. Thus, to achieve higher pH at the root surface in retreated teeth, it is clinically advisable to remove more dentin from the inner walls and to use normal saline as a vehicle for calcium hydroxide rather than acidic pH materials. PMID:23112482

The effect of retreatment procedure on the pH changes at the surface of root dentin using two different calcium hydroxide pastes.

PubMed

Kazemipoor, Maryam; Tabrizizadeh, Mehdi; Dastani, Milad; Hakimian, Roqayeh

2012-10-01

To compare pH changes at the cervical, middle and apical surfaces of root dentin in retreated and non- retreated teeth, after canal obturation with two different calcium hydroxide pastes. After instrumentation of 55 extracted teeth, three cavities with 0.75 mm depth and 1.5 mm in diameter were drilled at buccal root surface. The teeth were randomly divided into five groups. Canals in the first two groups were filled with either mixture of calcium hydroxide and saline solution and calcium hydroxide and 2% chlorhexidine (CHX). In the third and fourth groups canals were first obturated with gutta-percha and AH26 sealer, and then materials were removed. After 2 days canals were filled with two different calcium hydroxide pastes similar to the first and the second groups. The pH was measured in the prepared cavities at 1, 3, 7 and 14 days. In the non-retreated groups, pH at the surface of the roots was significantly higher in comparison to the retreated ones (P value < 0.001). pH values were significantly higher in the non-retreated teeth filling with calcium hydroxide and saline solution (P value < 0.001). Regarding to the little pH changes at the surface of dentin in retreated teeth, the hydroxyl ions cannot penetrate into the dentinal tubules. Thus, to achieve higher pH at the root surface in retreated teeth, it is clinically advisable to remove more dentin from the inner walls and to use normal saline as a vehicle for calcium hydroxide rather than acidic pH materials.

Extremophiles and biotechnology: current uses and prospects

PubMed Central

Coker, James A.

2016-01-01

Biotechnology has almost unlimited potential to change our lives in very exciting ways. Many of the chemical reactions that produce these products can be fully optimized by performing them at extremes of temperature, pressure, salinity, and pH for efficient and cost-effective outcomes. Fortunately, there are many organisms (extremophiles) that thrive in extreme environments found in nature and offer an excellent source of replacement enzymes in lieu of mesophilic ones currently used in these processes. In this review, I discuss the current uses and some potential new applications of extremophiles and their products, including enzymes, in biotechnology. PMID:27019700

Biotic and abiotic studies on the biological fate, transport and ecotoxicity of toxic and hazardous waste in the Mississippi River basin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Abdelghani, A.; Pramar, Y.; Mandal, T.

1996-05-02

This project assesses the levels of xenobiotics in Devils Swamp and studies their biological fate, transport, ecotoxicity, and potential toxicity to man. This article reports on the following studies: assessment of the acute toxicity of individual xenobiotics and toxicity of organic compounds hexachlorobutadience (HCB) and hexachlorobenzene (HCBD) on juvenile crayfish; determination of the biotic influence of temperature, salinity, pH, oxidation-reduction potential, and sediment composition on the migration of xenobiotics; development of a pharmacokinetics model for xenobiotic absorption and storage, distribution and excretion by fish and crayfish.

Salinity and Temperature Tolerance of the Nemertean Worm Carcinonemertes errans, an Egg Predator of the Dungeness Crab.

PubMed

Dunn, Paul H; Young, Craig M

2015-04-01

Estuaries can be harsh habitats for the marine animals that enter them, but they may also provide these species with sub-saline refuges from their parasites. The nemertean egg predator Carcinonemertes errans is known to occur less frequently and in smaller numbers on its host, the Dungeness crab Metacarcinus magister, when the hosts are found within estuaries. We examined the temperature and salinity tolerances of C. errans to determine if this observed distribution represents a true salinity refuge. We monitored the survival of juvenile and larval worms exposed to ecologically relevant salinities (5-30) and temperatures (8-20 °C) over the course of several days under laboratory conditions. Juvenile worms were unaffected by the experimental temperature levels and exhibited robustness to salinity treatments 25 and 30. However, significant mortality was seen at salinity treatments 20 and below. Larvae were less tolerant than juveniles to lowered salinity and were also somewhat more susceptible to the higher temperatures tested. Given that the Dungeness crab can tolerate forays into mesohaline (salinity 5-18) waters for several days at a time, our findings suggest that salinity gradients play an important role in creating a parasite refuge for this species within the estuaries of the Pacific Northwest. © 2015 Marine Biological Laboratory.

Effects of acute changes in salinity and temperature on routine metabolism and nitrogen excretion in gambusia (Gambusia affinis) and zebrafish (Danio rerio).

PubMed

Uliano, E; Cataldi, M; Carella, F; Migliaccio, O; Iaccarino, D; Agnisola, C

2010-11-01

Acute stress may affect metabolism and nitrogen excretion as part of the adaptive response that allows animals to face adverse environmental changes. In the present paper the acute effects of different salinities and temperatures on routine metabolism, spontaneous activity and excretion of ammonia and urea were studied in two freshwater fish: gambusia, Gambusia affinis and zebrafish, Danio rerio, acclimated to 27 degrees C. The effects on gill morphology were also evaluated. Five salinities (0 per thousand, 10 per thousand, 20 per thousand, 30 per thousand and 35 per thousand) were tested in gambusia, while four salinities were used in zebrafish (0 per thousand, 10 per thousand, 20 per thousand and 25 per thousand). Each salinity acute stress was tested alone or in combination with an acute temperature reduction to 20 degrees C. In gambusia, both salinity and temperature acute stress strongly stimulated urea excretion. Routine oxygen consumption was barely affected by acute salinity or temperature stress, and was reduced by the combined effects of temperature and high salinity. Gills maintained their structural integrity in all stressing conditions; hyperplasia and hypertrophy of mitochondria-rich cells were observed. In zebrafish, temperature and salinity acute changes, both alone and in combination, scarcely affected any parameter tested. The major effect observed was a reduction of nitrogen excretion at 20 degrees C-25 per thousand; under these extreme conditions a significant structural disruption of gills was observed. These results confirm the high tolerance to acute salinity and temperature stress in gambusia, and demonstrate the involvement of urea excretion modulation in the stress response in this species. Copyright 2010 Elsevier Inc. All rights reserved.

The pH of water from various sources: an overview for recommendation for patients with atopic dermatitis.

PubMed

Kulthanan, Kanokvalai; Nuchkull, Piyavadee; Varothai, Supenya

2013-07-01

Patients with atopic dermatitis (AD) have increased susceptibility to irritants. Some patients have questions about types of water for bathing or skin cleansing. We studied the pH of water from various sources to give an overview for physicians to recommend patients with AD. Water from various sources was collected for measurement of the pH using a pH meter and pH-indicator strips. Bottled drinking still water had pH between 6.9 and 7.5 while the sparkling type had pH between 4.9 and 5.5. Water derived from home water filters had an approximate pH of 7.5 as same as tap water. Swimming pool water had had pH between 7.2 and 7.5 while seawater had a pH of 8. Normal saline and distilled water had pH of 5.4 and 5.7, respectively. Facial mineral water had pH between 7.5 and 8, while facial makeup removing water had an acidic pH. Normal saline, distilled water, bottled sparkling water and facial makeup removing water had similar pH to that of normal skin of normal people. However, other factors including benefits of mineral substances in the water in terms of bacteriostatic and anti-inflammation should be considered in the selection of cleansing water.

The pH of water from various sources: an overview for recommendation for patients with atopic dermatitis

PubMed Central

Kulthanan, Kanokvalai; Varothai, Supenya

2013-01-01

Background Patients with atopic dermatitis (AD) have increased susceptibility to irritants. Some patients have questions about types of water for bathing or skin cleansing. Objective We studied the pH of water from various sources to give an overview for physicians to recommend patients with AD. Methods Water from various sources was collected for measurement of the pH using a pH meter and pH-indicator strips. Results Bottled drinking still water had pH between 6.9 and 7.5 while the sparkling type had pH between 4.9 and 5.5. Water derived from home water filters had an approximate pH of 7.5 as same as tap water. Swimming pool water had had pH between 7.2 and 7.5 while seawater had a pH of 8. Normal saline and distilled water had pH of 5.4 and 5.7, respectively. Facial mineral water had pH between 7.5 and 8, while facial makeup removing water had an acidic pH. Conclusion Normal saline, distilled water, bottled sparkling water and facial makeup removing water had similar pH to that of normal skin of normal people. However, other factors including benefits of mineral substances in the water in terms of bacteriostatic and anti-inflammation should be considered in the selection of cleansing water. PMID:23956962

Toxicity of antifouling biocides to the intertidal harpacticoid copepod Tigriopus japonicus (Crustacea, Copepoda): effects of temperature and salinity.

PubMed

Kwok, K W H; Leung, K M Y

2005-01-01

Intertidal harpacticoid copepods are commonly used in eco-toxicity tests worldwide. They predominately live in mid-high shore rock pools and often experience a wide range of temperature and salinity fluctuation. Most eco-toxicity tests are conducted at fixed temperature and salinity and thus the influence of these environmental factors on chemical toxicity is largely unknown. This study investigated the combined effect of temperature and salinity on the acute toxicity of the copepod Tigriopus japonicus against two common biocides, copper (Cu) and tributyltin (TBT) using a 2 x 3 x 4 factorial design (i.e. two temperatures: 25 and 35 degrees C; three salinities: 15.0 per thousand, 34.5 per thousand and 45.0 per thousand; three levels of the biocide plus a control). Copper sulphate and tributyltin chloride were used as the test chemicals while distilled water and acetone were utilised as solvents for Cu and TBT respectively. 96 h-LC50s of Cu and TBT were 1024 and 0.149 microg l(-1) respectively (at 25 degrees C; 34.5 per thousand) and, based on these results, nominal biocide concentrations of LC0 (i.e. control), LC30, LC50 and LC70 were employed. Analysis of Covariance using 'concentration' as the covariate and both 'temperature' and 'salinity' as fixed factors, showed a significant interaction between temperature and salinity effects for Cu, mortality increasing with temperature but decreasing with elevated salinity. A similar result was revealed for TBT. Both temperature and salinity are, therefore, important factors affecting the results of acute eco-toxicity tests using these marine copepods. We recommend that such eco-toxicity tests should be conducted at a range of environmentally realistic temperature/salinity regimes, as this will enhance the sensitivity of the test and improve the safety margin in line with the precautionary principle.

Elevated temperature enhances normal early embryonic development in the coral Platygyra acuta under low salinity conditions

NASA Astrophysics Data System (ADS)

Chui, Apple Pui Yi; Ang, Put

2015-06-01

To better understand the possible consequences of climate change on reef building scleractinian corals in a marginal environment, laboratory experiments were conducted to examine the interactive effects of changes in salinity and temperature on percent fertilization success and early embryonic development of the coral Platygyra acuta. In the present study, a salinity of 24 psu (ambient 32 psu) reduced fertilization success by 60 %. Normal embryonic development was reduced by >80 % at 26 psu (ambient 33 psu) with 100 % abnormal development at 22 psu under ambient temperature. Elevated temperature (+3 °C) above the ambient spawning temperature did not show any negative effects on fertilization success. However, there was a trend for more abnormal embryos to develop at elevated temperature in the 2 d of the spawning event. The interactive effects between salinity and temperature are statistically significant only on normal embryonic development of P. acuta, but not on its fertilization success. Salinity was revealed to be the main factor affecting both fertilization success and normal embryonic development. Interestingly, the much lower fertilization success (76 %) observed in the second day of spawning (Trial 2) under ambient temperature recovered to 99 % success under elevated (+3 °C) temperature conditions. Moreover, elevated temperature enhanced normal early embryonic development under lowered salinity (26 psu). This antagonistic interactive effect was consistently observed in two successive nights of spawning. Overall, our results indicate that, in terms of its fertilization success and embryonic development, P. acuta is the most tolerant coral species to reduced salinity thus far reported in the literature. Elevated temperature, at least that within the tolerable range of the corals, could apparently alleviate the potential negative effects from salinity stresses. This mitigating role of elevated temperature appears not to have been reported on corals before.

Optimisation of critical medium components and culture conditions for enhanced biomass and lipid production in the oleaginous diatom Navicula phyllepta: a statistical approach.

PubMed

Sabu, Sanyo; Singh, Isaac Sarojini Bright; Joseph, Valsamma

2017-12-01

Diatoms hold great promise as potential sources of biofuel production. In the present study, the biomass and lipid production in the marine diatom Navicula phyllepta, isolated from Cochin estuary, India and identified as a potential biodiesel feedstock, were optimized using Plackett-Burman (PB) statistical experimental design followed by central composite design (CCD) and response surface methodology (RSM). The growth analyses of the isolate in different nitrogen sources, salinities and five different enriched sea water media showed the best growth in the cheapest medium with minimum components using urea as nitrogen source at salinity between 25 and 40 g kg -1 . Plackett-Burman experimental analyses for screening urea, sodium metasilicate, sodium dihydrogen phosphate, ferric chloride, salinity, temperature, pH and agitation influencing lipid and biomass production showed that silicate and temperature had a positive coefficient on biomass production, and temperature had a significant positive coefficient, while urea and phosphate showed a negative coefficient on lipid content. A 2 4 factorial central composite design (FCCD) was used to optimize the concentration of the factors selected. The optimized media resulted in 1.62-fold increase (64%) in biomass (1.2 ± 0.08 g L -1 ) and 1.2-fold increase (22%) in estimated total lipid production (0.11 ± 0.003 g L -1 ) compared to original media within 12 days of culturing. A significantly higher biomass and lipid production in the optimized medium demands further development of a two-stage strategy of biomass production followed by induction of high lipid production under nutrient limitation or varying culture conditions for large-scale production of biodiesel from the marine diatom.

The effects of salinity and temperature shock on Kappaphycus alvarezii seaweed spores release

NASA Astrophysics Data System (ADS)

Harwinda, F. K.; Satyantini, W. H.; Masithah, E. W.

2018-04-01

One of the reproductive aspects of development step that is considered as the solution of this issue is seaweed sporulation technique through which is induced through salinity and temperature shock. This study aims to determine the effect of combination and interaction of salinity and temperature shock on the release of K. alvarezii spores in order to produce superior seeds. This research was conducted using Complete Randomized Design Factorial which consists of nine combinations of treatments and three replications. The used treatment in this study is the combination of different environmental factors such as salinity shock and temperature shock. The data were analyzed using ANOVA (Analysis of Variance) followed by Duncan Multiple Range Test. The results showed that salinity (31 ppt, 33 ppt, and 35 ppt) and temperature (30°C, 32°C, and 34°C). shock affected the osmoregulation system and the release of K. alvarezii spores. The salinity shock and temperature shock had interaction with K. alvarezii spore release on the sixth and seventh day with the best treatment at 32°C temperature and 31 ppt salinity and released 5413 cells/ml spores on the seventh day.

Mesolimbic and Nigrostriatal Dopaminergic Systems: Behavioral Neuropharmacology.

DTIC Science & Technology

1985-08-01

presented in Table Table III List of drugs D ru gVeh i c l e Intracerebral infusions Dopamine agonist~s Apomorphine hydrochloride 0.1% Na metabisulfite...saline GABA 0.9% saline Picrotoxin 0 .9%saline Systemic injections Dopamine agents d-Amphetamine sulfate 0.9% saline Aponiorphine hydrochloride 0.9...3H)methionine (15 Ci/mmole, lmCi/ml. 16 Amersham), 122 ul of freshly prepared pargyline hydrochloride (10.2 mM), 326 ul of I M Tris pH 10.8, 246 ul

Gypsum addition to soils contaminated by red mud: implications for aluminium, arsenic, molybdenum and vanadium solubility.

PubMed

Lehoux, Alizée P; Lockwood, Cindy L; Mayes, William M; Stewart, Douglas I; Mortimer, Robert J G; Gruiz, Katalin; Burke, Ian T

2013-10-01

Red mud is highly alkaline (pH 13), saline and can contain elevated concentrations of several potentially toxic elements (e.g. Al, As, Mo and V). Release of up to 1 million m(3) of bauxite residue (red mud) suspension from the Ajka repository, western Hungary, caused large-scale contamination of downstream rivers and floodplains. There is now concern about the potential leaching of toxic metal(loid)s from the red mud as some have enhanced solubility at high pH. This study investigated the impact of red mud addition to three different Hungarian soils with respect to trace element solubility and soil geochemistry. The effectiveness of gypsum amendment for the rehabilitation of red mud-contaminated soils was also examined. Red mud addition to soils caused a pH increase, proportional to red mud addition, of up to 4 pH units (e.g. pH 7 → 11). Increasing red mud addition also led to significant increases in salinity, dissolved organic carbon and aqueous trace element concentrations. However, the response was highly soil specific and one of the soils tested buffered pH to around pH 8.5 even with the highest red mud loading tested (33 % w/w); experiments using this soil also had much lower aqueous Al, As and V concentrations. Gypsum addition to soil/red mud mixtures, even at relatively low concentrations (1 % w/w), was sufficient to buffer experimental pH to 7.5-8.5. This effect was attributed to the reaction of Ca(2+) supplied by the gypsum with OH(-) and carbonate from the red mud to precipitate calcite. The lowered pH enhanced trace element sorption and largely inhibited the release of Al, As and V. Mo concentrations, however, were largely unaffected by gypsum induced pH buffering due to the greater solubility of Mo (as molybdate) at circumneutral pH. Gypsum addition also leads to significantly higher porewater salinities, and column experiments demonstrated that this increase in total dissolved solids persisted even after 25 pore volume replacements. Gypsum addition could therefore provide a cheaper alternative to recovery (dig and dump) for the treatment of red mud-affected soils. The observed inhibition of trace metal release within red mud-affected soils was relatively insensitive to either the percentage of red mud or gypsum present, making the treatment easy to apply. However, there is risk that over-application of gypsum could lead to detrimental long-term increases in soil salinity.

Virgibacillus ainsalahensis sp. nov., a Moderately Halophilic Bacterium Isolated from Sediment of a Saline Lake in South of Algeria.

PubMed

Amziane, Meriam; Darenfed-Bouanane, Amel; Abderrahmani, Ahmed; Selama, Okba; Jouadi, Lydia; Cayol, Jean-Luc; Nateche, Farida; Fardeau, Marie-Laure

2017-02-01

A Gram-positive, moderately halophilic, endospore-forming bacterium, designated MerV T , was isolated from a sediment sample of a saline lake located in Ain Salah, south of Algeria. The cells were rod shaped and motile. Isolate MerV T grew at salinity interval of 0.5-25% NaCl (optimum, 5-10%), pH 6.0-12.0 (optimum, 8.0), and temperature between 10 and 40 °C (optimum, 30 °C).The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, a glycolipid, a phospholipid, and two lipids, and MK-7 is the predominant menaquinone. The predominant cellular fatty acids were anteiso C 15:0 and anteiso C 17:0 . The DNA G+C content was 45.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain MerV T was most closely related to Virgibacillus halodenitrificans (gene sequence similarity of 97.0%). On the basis of phenotypic, chemotaxonomic properties, and phylogenetic analyses, strain MerV T (=DSM = 28944 T ) should be placed in the genus Virgibacillus as a novel species, for which the name Virgibacillus ainsalahensis is proposed.

Measurements of the dielectric properties of sea water at 1.43 GHz

NASA Technical Reports Server (NTRS)

Ho, W. W.; Love, A. W.; Vanmelle, M. J.

1974-01-01

Salinity and temperature of water surfaces of estuaries and bay regions are determined to accuracies of 1 ppt salinity and 0.3 kelvin surface temperature. L-band and S-band radiometers are used in combination as brightness temperature detectors. The determination of the brightness temperature versus salinity, with the water surface temperature as a parameter for 1.4 GHz, is performed with a capillary tube inserted into a resonance cavity. Detailed analysis of the results indicates that the measured values are accurate to better than 0.2 percent in the electric property epsilon' and 0.4 percent in epsilon''. The calculated brightness temperature as a function of temperature and salinity is better than 0.2 kelvin. Thus it is possible to reduce the measured data obtained with the two-frequency radiometer system with 1 ppt accuracy to values in the salinity range 5 to 40 ppt.

Effects of Seawater Salinity and Temperature on Growth and Pigment Contents in Hypnea cervicornis J. Agardh (Gigartinales, Rhodophyta)

PubMed Central

Ding, Lanping; Ma, Yuanyuan; Huang, Bingxin; Chen, Shanwen

2013-01-01

This study simulated outdoor environmental living conditions and observed the growth rates and changes of several photosynthetic pigments (Chl a, Car, PE, and PC) in Hypnea cervicornis J. Agardh (Gigartinales, Rhodophyta) by setting up different ranges of salinity (25, 30, 35, 40, 45, and 50) and temperature (15, 20, 25, and 30°C). At conditions of culture, the results are as follows. (1) Changes in salinity and temperature have significant effects on the growth of H. cervicornis. The growth rates first increase then decrease as the temperature increases, while growth tends to decline as salinity increases. The optimum salinity and temperature conditions for growth are 25 and 25°C, respectively. (2) Salinity and temperature have significant or extremely significant effects on photosynthetic pigments (Chl a, Car, PE, and PC) in H. cervicornis. The results of this study are advantageous to ensure propagation and economic development of this species in the southern sea area of China. PMID:24350276

Effect of temperature and salinity on phosphate sorption on marine sediments.

PubMed

Zhang, Jia-Zhong; Huang, Xiao-Lan

2011-08-15

Our previous studies on the phosphate sorption on sediments in Florida Bay at 25 °C in salinity 36 seawater revealed that the sorption capacity varies considerably within the bay but can be attributed to the content of sedimentary P and Fe. It is known that both temperature and salinity influence the sorption process and their natural variations are the greatest in estuaries. To provide useful sorption parameters for modeling phosphate cycle in Florida Bay, a systematic study was carried out to quantify the effects of salinity and temperature on phosphate sorption on sediments. For a given sample, the zero equilibrium phosphate concentration and the distribution coefficient were measured over a range of salinity (2-72) and temperature (15-35 °C) conditions. Such a suite of experiments with combinations of different temperature and salinity were performed for 14 selected stations that cover a range of sediment characteristics and geographic locations of the bay. Phosphate sorption was found to increase with increasing temperature or decreasing salinity and their effects depended upon sediment's exchangeable P content. This study provided the first estimate of the phosphate sorption parameters as a function of salinity and temperature in marine sediments. Incorporation of these parameters in water quality models will enable them to predict the effect of increasing freshwater input, as proposed by the Comprehensive Everglades Restoration Plan, on the seasonal cycle of phosphate in Florida Bay.

Characterization of halophilic C50 carotenoid-producing archaea isolated from solar saltworks in Bohai Bay, China

NASA Astrophysics Data System (ADS)

Sui, Liying; Liu, Liangsen; Deng, Yuangao

2014-11-01

Halophilic archaea comprise the majority of microorganisms found in hypersaline environments. C50 carotenoids accumulated in archaea cells are considered potential biotechnological products and possess a number of biological functions. Ten red colonies were isolated from brine water in a saltern crystallizer pond of the Hangu Saltworks, China. 16S rRNA gene sequence analysis showed that the colonies belonged to the extremely halophilic archaea genera Halobacterium and Halorubrum. Two representative strains, Halobacterium strain SP-2 and Halorubrum strain SP-4, were selected for further study on the phenotypic characteristics and effects of salinity and pH on accumulation and composition of pigments in their cells. The archaeal strains were isolated and grown in a culture medium prepared by dissolving yeast extract (10 g/L) and acid-hydrolyzed casein (7.5 g/L) into brine water obtained from a local salt pond. Their optimum salinity and pH for growth were 250 and 7, respectively, although pigment accumulation (OD490 / mL broth) was highest at pH 8. In addition, at 150-300 salinity, increasing salinity resulted in decreasing pigment accumulation. Analysis of the UV-Vis spectrum, TLC and HLPC chromatograms showed that C50 carotenoid bacterioruberin is the major pigment in both strains.

Simulating the role of surface forcing on observed multidecadal upper-ocean salinity changes

DOE PAGES

Lago, Veronique; Wijffels, Susan E.; Durack, Paul J.; ...

2016-07-18

The ocean’s surface salinity field has changed over the observed record, driven by an intensification of the water cycle in response to global warming. However, the origin and causes of the coincident subsurface salinity changes are not fully understood. The relationship between imposed surface salinity and temperature changes and their corresponding subsurface changes is investigated using idealized ocean model experiments. The ocean’s surface has warmed by about 0.5°C (50 yr) –1 while the surface salinity pattern has amplified by about 8% per 50 years. The idealized experiments are constructed for a 50-yr period, allowing a qualitative comparison to the observedmore » salinity and temperature changes previously reported. The comparison suggests that changes in both modeled surface salinity and temperature are required to replicate the three-dimensional pattern of observed salinity change. The results also show that the effects of surface changes in temperature and salinity act linearly on the changes in subsurface salinity. In addition, surface salinity pattern amplification appears to be the leading driver of subsurface salinity change on depth surfaces; however, surface warming is also required to replicate the observed patterns of change on density surfaces. This is the result of isopycnal migration modified by the ocean surface warming, which produces significant salinity changes on density surfaces.« less

Simulating the role of surface forcing on observed multidecadal upper-ocean salinity changes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lago, Veronique; Wijffels, Susan E.; Durack, Paul J.

The ocean’s surface salinity field has changed over the observed record, driven by an intensification of the water cycle in response to global warming. However, the origin and causes of the coincident subsurface salinity changes are not fully understood. The relationship between imposed surface salinity and temperature changes and their corresponding subsurface changes is investigated using idealized ocean model experiments. The ocean’s surface has warmed by about 0.5°C (50 yr) –1 while the surface salinity pattern has amplified by about 8% per 50 years. The idealized experiments are constructed for a 50-yr period, allowing a qualitative comparison to the observedmore » salinity and temperature changes previously reported. The comparison suggests that changes in both modeled surface salinity and temperature are required to replicate the three-dimensional pattern of observed salinity change. The results also show that the effects of surface changes in temperature and salinity act linearly on the changes in subsurface salinity. In addition, surface salinity pattern amplification appears to be the leading driver of subsurface salinity change on depth surfaces; however, surface warming is also required to replicate the observed patterns of change on density surfaces. This is the result of isopycnal migration modified by the ocean surface warming, which produces significant salinity changes on density surfaces.« less

Survival, growth and reproduction of non-indigenous Nile tilapia, Oreochromis niloticus (Linnaeus 1758). I. Physiological capabilities in various temperatures and salinities

USGS Publications Warehouse

Schofield, Pamela J.; Peterson, Mark S.; Lowe, Michael R.; Brown-Peterson, Nancy J.; Slack, William T.

2011-01-01

The physiological tolerances of non-native fishes is an integral component of assessing potential invasive risk. Salinity and temperature are environmental variables that limit the spread of many non-native fishes. We hypothesised that combinations of temperature and salinity will interact to affect survival, growth, and reproduction of Nile tilapia, Oreochromis niloticus, introduced into Mississippi, USA. Tilapia withstood acute transfer from fresh water up to a salinity of 20 and survived gradual transfer up to 60 at typical summertime (30°C) temperatures. However, cold temperature (14°C) reduced survival of fish in saline waters ≥10 and increased the incidence of disease in freshwater controls. Although fish were able to equilibrate to saline waters in warm temperatures, reproductive parameters were reduced at salinities ≥30. These integrated responses suggest that Nile tilapia can invade coastal areas beyond their point of introduction. However, successful invasion is subject to two caveats: (1) wintertime survival depends on finding thermal refugia, and (2) reproduction is hampered in regions where salinities are ≥30. These data are vital to predicting the invasion of non-native fishes into coastal watersheds. This is particularly important given the predicted changes in coastal landscapes due to global climate change and sea-level rise.

Interpretation of time series (salinity and temperature) layers in North Atlantic from 1950 to 2011

NASA Astrophysics Data System (ADS)

Rubchenia, A.; Popov, A.; Fedorova, A.; Lebedev, N.

2012-04-01

On the basis of long period data series (1950-2011) form various sources (National Oceanographic Data Center (NOAD) (www.nodc.noaa.gov), WOD09 database and data from ARGO project) 10 boxes in North Atlantic were selected. Location of boxes was determined by circulation pattern in North Atlantic and Euro-Arctic Seas. For further analysis two "seasons" was selected: "cold season" (October-May) and "warm season" (June-September). Data verification was made. Analysis of data series clearly show the "Great Salinity Anomaly" (so-called GSA) in 1960-70th, 1980th and 1990th. Trends of salinity and temperature data series were calculated. Spectral analysis allow us to calculate periodicity from 2 to 22 years. Boxes situated in regions with Arctic waters have singularity showed through domination of high frequency oscillation during propagation to South. In Fram Strait salinity fluctuates with periods 9..11 and 20 years, the same period was calculated using temperature data series. In Denmark Strait there are oscillations of temperature with specific period from 4 to 7 years. Range of variability vary. For salinity it is 0.4..4.6 psu, for temperature it is 0.04..5.5C. In salinity data series from boxes with surface Arctic waters noticed clear minimums connected with GSAs. Trends in Denmark Strait and Fram strait in the end of 2000th are negative at different levels. Since 1975 to 2001 salinity near the southern part of Greenland was increased, since 2001 - decreased. But temperature was raised from 0.04 in 1989 to 5.59 in 2010. Thermohaline characteristics of water masses which has Atlantic origin oscillated with period near 20 years. Salinity near Newfoundland was decreased since 2005. In Farrero-Shetland straits salinity trend is positive since at 100m level, Salinity rising from 1970th to 2006 is about 0.3 psu were noticed. Oscillations with period 2..4 years is weak. But at 800m layer salinity oscillations are different, since 1990 there is not significant oscillations at all. Temperature trend at this level is negative since 1950th. Salinity at 100-300 level at Station M area described with negative trend since 1960 to 1993, in both "seasons". Next, up to 2010 salinity is increasing, but in 2011 salinity dramatically decreased. Main oscillations have periods 2..3 years, 4..5 years and 20 years. At 800m level oscillations are very weak. Temperature is increased since 1995 in surface layer and since 2002 in deeper levels. At all levels temperature dramatically decreased after 2010. In central part of Greenland Sea ("Cupola area") dominated oscillations with period 4 years (1950-60th), 5..7 years (1970th) and 9 years (after 1979). In "cold season" oscillation with 11 years traced. Salinity trend is positive at all levels during last 10-15 years. Salinity and temperature were increased at 800m level up to 2006. It could lead to termination of deep water formation. Since 2006 temperature decreased, especially in "cold season". Salinity trend in West Spitsbergen Current is positive since 1996 at surface and sine 1978 at deeper levels. Temperature was increased since 1965 to 2006 in surface layer, but since 2006 in "warm season" temperature is decreasing at all layers. Main oscillations is 4..5 years, 6..7 years and 9..11 years.

Influence of intramuscular granisetron on experimentally induced muscle pain by acidic saline.

PubMed

Louca, S; Ernberg, M; Christidis, N

2013-06-01

The aim of this study was to investigate whether intramuscular administration of the 5-HT(3) receptor antagonist granisetron reduces experimental muscle pain induced by repeated intramuscular injections of acidic saline into the masseter muscles. Twenty-eight healthy and pain-free volunteers, fourteen women and fourteen men participated in this randomized, double-blind and placebo-controlled study. After a screening examination and registration of the baseline pressure-pain threshold (PPT), the first simultaneous bilateral injections of 0·5 mL acidic saline (9 mg mL(-1) , pH 3·3) into the masseter muscles were performed. Two days later, PPT and pain (VAS) were re-assessed. The masseter muscle was then pre-treated with 0·5 mL granisetron (Kytril(®) 1 mg mL(-1) pH 5·3) on one side and control substance (isotonic saline, 9 mg mL(-1) pH 6) on the contralateral side. Two minutes thereafter a bilateral simultaneous injection of 0·5 mL acidic saline followed. The evoked pain intensity, pain duration, pain area and PPT were assessed. The volunteers returned 1 week later to re-assess VAS and PPT. On the side pre-treated with granisetron, the induced pain had significantly lower intensity and shorter duration (P < 0·05) compared with the side pre-treated with control. A subgroup analysis showed that the effect of granisetron on pain duration was significant only in women (P < 0·001), while the effect on peak pain and pain area were significant in both sexes. The results showed no significant change in PPT. In conclusion, these results indicate that granisetron has a pain-reducing effect on experimentally induced muscle pain by repeated acidic saline injection. © 2013 John Wiley & Sons Ltd.

A Mosaic of Geothermal and Marine Features Shapes Microbial Community Structure on Deception Island Volcano, Antarctica

PubMed Central

Bendia, Amanda G.; Signori, Camila N.; Franco, Diego C.; Duarte, Rubens T. D.; Bohannan, Brendan J. M.; Pellizari, Vivian H.

2018-01-01

Active volcanoes in Antarctica contrast with their predominantly cold surroundings, resulting in environmental conditions capable of selecting for versatile and extremely diverse microbial communities. This is especially true on Deception Island, where geothermal, marine, and polar environments combine to create an extraordinary range of environmental conditions. Our main goal in this study was to understand how microbial community structure is shaped by gradients of temperature, salinity, and geochemistry in polar marine volcanoes. Thereby, we collected surface sediment samples associated with fumaroles and glaciers at two sites on Deception, with temperatures ranging from 0 to 98°C. Sequencing of the 16S rRNA gene was performed to assess the composition and diversity of Bacteria and Archaea. Our results revealed that Deception harbors a combination of taxonomic groups commonly found both in cold and geothermal environments of continental Antarctica, and also groups normally identified at deep and shallow-sea hydrothermal vents, such as hyperthermophilic archaea. We observed a clear separation in microbial community structure across environmental gradients, suggesting that microbial community structure is strongly niche driven on Deception. Bacterial community structure was significantly associated with temperature, pH, salinity, and chemical composition; in contrast, archaeal community structure was strongly associated only with temperature. Our work suggests that Deception represents a peculiar “open-air” laboratory to elucidate central questions regarding molecular adaptability, microbial evolution, and biogeography of extremophiles in polar regions. PMID:29867810

Physico-chemical conditions for plankton in Lake Timsah, a saline lake on the Suez Canal

NASA Astrophysics Data System (ADS)

El-Serehy, H. A. H.; Sleigh, M. A.

1992-02-01

Lake Timsah receives high salinity water from the Suez Canal, mainly from the south, and freshwater from a Nile canal and other sources, producing a salinity stratification with surface salinities of 20-40‰ and over 40‰ in deeper water. Water temperature at a depth of 50-70 cm fell to below 20 °C in winter and rose to above 30 °C in summer; oxygen concentration at the same depth ranged between 6-10 mg l -1 and the pH was 8·1-8·3, and at mid-day this water was supersaturated with oxygen through 6-8 months of the year. The main chemical nutrients reached their highest levels in winter (December-February) and their lowest levels in summer (May-August), silicate varying between 1-7 μ M, phosphate between 0·1 and 0·8 μ M and nitrate between 4-10 μ M; nitrite varied in a more complex manner, usually between 0·25 and 0·4 μ M. The atomic ratio of N/P was generally well above the Redfield ratio level, except for a few months in midwinter. These nutrient concentrations are high in comparison with those of unpolluted seas of the region, but are typical of the more eutrophic coastal waters in most parts of the world.

Relationship between white spot syndrome virus (WSSV) loads and characterizations of water quality in Litopenaeus vannamei culture ponds during the tropical storm.

PubMed

Zhang, J S; Li, Z J; Wen, G L; Wang, Y L; Luo, L; Zhang, H J; Dong, H B

2016-01-01

An in-situ experiment was conducted to investigate the effect of tropical storm on the white spot syndrome virus (WSSV) loads in Litopenaeus vannamei rearing ponds. White spot syndrome virus loads, heterotrophic bacteria, Vibrio and water quality (including temperature, dissolved oxygen (DO), salinity, pH, NH 4 -N, and NO 2 -N) were continually monitored through one tropical storm. The WSSV loads decreased when tropical storm made landfall, and substantially increased when typhoon passed. The variation of WSSV loads was correlated with DO, temperature, heterotrophic bacteria count, and ammonia-N concentrations. These results suggested that maintaining high level DO and promoting heterotrophic bacteria growth in the shrimp ponds might prevent the diseases' outbreak after the landfall of tropical storm.

Relationship between white spot syndrome virus (WSSV) loads and characterizations of water quality in Litopenaeus vannamei culture ponds during the tropical storm

PubMed Central

Zhang, J. S.; Li, Z. J.; Wen, G. L.; Wang, Y. L.; Luo, L.; Zhang, H. J.; Dong, H. B.

2016-01-01

An in-situ experiment was conducted to investigate the effect of tropical storm on the white spot syndrome virus (WSSV) loads in Litopenaeus vannamei rearing ponds. White spot syndrome virus loads, heterotrophic bacteria, Vibrio and water quality (including temperature, dissolved oxygen (DO), salinity, pH, NH4-N, and NO2-N) were continually monitored through one tropical storm. The WSSV loads decreased when tropical storm made landfall, and substantially increased when typhoon passed. The variation of WSSV loads was correlated with DO, temperature, heterotrophic bacteria count, and ammonia-N concentrations. These results suggested that maintaining high level DO and promoting heterotrophic bacteria growth in the shrimp ponds might prevent the diseases’ outbreak after the landfall of tropical storm. PMID:27822254

Environmental controls on the boron and strontium isotopic composition of aragonite shell material of cultured Arctica islandica

NASA Astrophysics Data System (ADS)

Liu, Y.-W.; Aciego, S. M.; Wanamaker, A. D., Jr.

2015-02-01

Ocean acidification, the decrease in ocean pH associated with increasing atmospheric CO2, is likely to impact marine organisms, particularly those that produce carbonate skeletons or shells. Therefore it is important to investigate how environmental factors (seawater pH, temperature and salinity) influence the chemical compositions in biogenic carbonates. In this study we report the first high-resolution strontium (87Sr / 86Sr and δ88 / 86Sr) and boron (δ11B) isotopic values in the aragonite shell of cultured Arctica islandica (A. islandica). The 87Sr / 86Sr ratios from both tank water and shell samples show ratios nearly identical to the open ocean, which suggests that the shell material reflects ambient ocean chemistry without terrestrial influence. The 84Sr-87Sr double spike resolved shell δ 88 / 86Sr and Sr concentration data show no resolvable change throughout the culture period and reflect no theoretical kinetic mass fractionation throughout the experiment despite a temperature change of more than 15 °C. The δ11B records from the experiment show at least a 5‰ increase through the culture season (January 2010-August 2010), with low values from beginning to week 19 and higher values hereafter. The larger range in δ11B in this experiment compared to predictions based on other carbonate organisms (2-3‰) suggests that a species-specific fractionation factor may be required. A relatively strong correlation between the Δ pH (pHshell-pHsw) and seawater pH (pHsw) was observed (R2 = 0.34), which suggests that A. islandica partly regulates the pH of the extrapallial fluid. However, this proposed mechanism only explains approximately 34% of the variance in the δ11B data. Instead, a rapid rise in δ11B after week 19 suggests that the boron uptake of the shell changes when a temperature threshold of 13 °C is reached.

Time course of the acute response of the North Pacific spiny dogfish shark (Squalus suckleyi) to low salinity.

PubMed

Guffey, Samuel C; Goss, Greg G

2014-05-01

Dogfish are considered stenohaline sharks but are known to briefly enter estuaries. The acute response of North Pacific spiny dogfish (Squalus suckleyi) to lowered salinity was tested by exposing sharks to 21‰ salinity for 48 h. Temporal trends in blood pH, plasma osmolality, CO2, HCO3(-), Na(+), Cl(-), K(+), and urea concentrations, and in the rates of urea efflux and O2 consumption, were quantified. The rate of O2 consumption exhibited cyclic variation and was significantly depressed by lowered salinity. After 9 h, plasma [Cl(-)] stabilized at 9% below initial levels, while plasma [Na(+)] decreased by more than 20% within the first 12 h. Plasma [urea] dropped by 15% between 4 and 6 h, and continued to decrease. The rate of urea efflux increased over time, peaking after 36 h at 72% above the initial rate. Free-swimming sharks subjected to the same salinity challenge survived over 96 h and differed from cannulated sharks with respect to patterns of Na(+) and urea homeostasis. This high-resolution study reveals that dogfish exposed to 21‰ salinity can maintain homeostasis of Cl(-) and pH, but Na(+) and urea continue to be lost, likely accounting for the inability of the dogfish to fully acclimate to reduced salinity. Copyright © 2014 Elsevier Inc. All rights reserved.

Studies of quaternary saline lakes-II. Isotopic and compositional changes during desiccation of the brines in Owens Lake, California, 1969-1971

USGS Publications Warehouse

Friedman, I.; Smith, G.I.; Hardcastle, Kenneth G.

1976-01-01

Owens Lake is an alkaline salt lake in a closed basin in southeast California. It is normally nearly dry, but in early 1969, an abnormal runoff from the Sierra Nevada flooded it to a maximum depth of 2??4 m. By late summer of 1971, the lake was again nearly dry and the dissolved salts recrystallized. Changes in the chemistry, pH, and deuterium content were monitored during desiccation. During flooding, salts (mostly trona, halite, and burkeite) dissolved slowly from the lake floor. Their concentration in the lake waters increased as evaporation removed water and salts again crystallized, but winter temperatures caused precipitation of some salts and the following summer warming caused their solution, resulting in seasonal variations in the concentration patterns of some ions. The pH values (9??4-10??4) changed with time but showed no detectable diurnal pattern. The deuterium concentration increased during evaporation and appeared to be in equilibrium with vapor leaving the lake according to the Rayleigh equation. The effective ??(D/H in liquid/D/H in vapor) decreased as salinity increased; the earliest measured value was 1??069 [as total dissolved solids (TDS) of lake waters changed from 136,200 to 250,400 mg/1]and the last value (calc.) was 1??025 (as TDS changed from 450,000 to 470,300 mg/1). Deuterium exchange with the atmosphere was apparently small except during late desiccation stages when the isotopic contrast became great. Eventually, atmospheric exchange, combined with decreasing ?? and lake size and increasing salinity, stopped further deuterium concentration in the lake. The maximum contrast between atmospheric vapor and lake deuterium contents was about 110%. ?? 1976.

Groundwater quality assessment in the village of Lutfullapur Nawada, Loni, District Ghaziabad, Uttar Pradesh, India.

PubMed

Singh, Vinod K; Bikundia, Devendra Singh; Sarswat, Ankur; Mohan, Dinesh

2012-07-01

The groundwater quality for drinking, domestic and irrigation in the village Lutfullapur Nawada, Loni, district Ghaziabad, U.P., India, has been assessed. Groundwater samples were collected, processed and analyzed for temperature, pH, conductivity, salinity, total alkalinity, carbonate alkalinity, bicarbonate alkalinity, total hardness, calcium hardness, magnesium hardness, total solids, total dissolved solids, total suspended solids, nitrate-nitrogen, chloride, fluoride, sulfate, phosphate, silica, sodium, potassium, calcium, magnesium, total chromium, cadmium, copper, iron, nickel, lead and zinc. A number of groundwater samples showed levels of electrical conductivity (EC), alkalinity, chloride, calcium, sodium, potassium and iron exceeding their permissible limits. Except iron, the other metals (Cr, Cd, Cu, Ni, Pb, and Zn) were analyzed below the permissible limits. The correlation matrices for 28 variables were performed. EC, salinity, TS and TDS had significant positive correlations among themselves and also with NO (3) (-) , Cl(-), alkalinity, Na(+), K(+), and Ca(2+). Fluoride was not significantly correlated with any of the parameters. NO (3) (-) was significantly positively correlated with Cl(-), alkalinity, Na(+), K(+) and Ca(2+). Chloride also correlated significantly with alkalinity, Na(+), K(+) and Ca(2+). Sodium showed a strong and positive correlation with K(+) and Ca(2+). pH was negatively correlated with most of the physicochemical parameters. This groundwater is classified as a normal sulfate and chloride type. Base-exchange indices classified 73% of the groundwater sources as the Na(+)-SO (4) (2-) type. The meteoric genesis indices demonstrated that 67% of groundwater sources belong to a deep meteoric water percolation type. Hydrochemical groundwater evaluations revealed that most of the groundwaters belong to the Na(+)-K(+)-Cl(-)-SO (4) (2-) type followed by Na(+)-K(+)-HCO (3) (-) type. Salinity, chlorinity and SAR indices indicated that majority of groundwater samples can be considered suitable for irrigation purposes.

Analysis of Heavy Metal Content (Pb) on Waters and Fish at The Floating Cages BPPP Ambon

NASA Astrophysics Data System (ADS)

Wattimena, Rachel L.; Selanno, Debby A. J.; Tuhumury, Semuel F.; Tuahatu, Juliana W.

2018-02-01

Coastal waters play important roles due to highly in natural resources and developing of environmental services. However, there are highly intensity of natural resources utilization, environment and settlement. Consequently, environment and natural resources would be degraded such as in the Ambon Bay. One of the potency at the Ambon Bay is mariculture area namely the floating cages (KJA) which belongs to Fisheries education and training (BPPP) Ambon. The research aimed to analyze physical-chemical of waters (temperature, pH, salinity and current speed), to analyze heavy metal concentration (Pb) on water and fish from floating cages (KJA) and to analyze waters pollution status at KJA BPPP Ambon. The average salinity of each floating cage ranged from 30.09 - 30.34°C, pH ranged from 8.03 - 8.44, salinity ranged from 31.36 - 33.34 PSU, and current speed at spring tide ranged from 0.5 - 55.8 Cm/sec while neap tide ranged from 0.1 - 9.8 Cm/sec. Heavy metal concentration (Pb) on waters was below the standard for waters quality and the average concentration was 0.002 mg/l. Whilst, the heavy metal concentration (Pb) on fishes was below standard for floating cages (floating cages 2-6) which was 0.05 and 0.17mg/l. Otherwise, floating cage 1 had been above maximum standard for fish food and its processing following SNI 7387:2009 (0.3mg/l) which was 0.31 mg/l. The status of waters pollution at KJA BPPP Ambon belonged to C class and could be categorized as moderate based on standard for waters quality issued by State Ministerial Decree for the Environment No. 51 Year 2004.

The Acid Test: pH Tolerance of the Eggs and Larvae of the Invasive Cane Toad (Rhinella marina) in Southeastern Australia.

PubMed

Wijethunga, Uditha; Greenlees, Matthew; Shine, Richard

2015-01-01

Invasive cane toads are colonizing southeastern Australia via a narrow coastal strip sandwiched between unsuitable areas (Pacific Ocean to the east, mountains to the west). Many of the available spawning sites exhibit abiotic conditions (e.g., temperature, salinity, and pH) more extreme than those encountered elsewhere in the toad's native or already invaded range. Will that challenge impede toad expansion? To answer that question, we measured pH in 35 ponds in northeastern New South Wales and 8 ponds in the Sydney region, in both areas where toads occur (and breed) and adjacent areas where toads are likely to invade, and conducted laboratory experiments to quantify effects of pH on the survival and development of toad eggs and larvae. Our field surveys revealed wide variation in pH (3.9-9.8) among natural water bodies. In the laboratory, the hatching success of eggs was increased at low pH (down to pH 4), whereas the survival, growth, and developmental rates of tadpoles were enhanced by higher pH levels. We found that pH influenced metamorph size and shape (relative head width, relative leg length) but not locomotor performance. The broad tolerance range of these early life-history stages suggests that pH conditions in ponds will not significantly slow the toad's expansion southward. Indeed, toads may benefit from transiently low pH conditions, and habitat where pH in wetlands is consistently low (such as coastal heath) may enhance rather than reduce toad reproductive success. A broad physiological tolerance during embryonic and larval life has contributed significantly to the cane toad's success as a widespread colonizer.

Salinity effect on the maximal growth temperature of some bacteria isolated from marine enviroments.

PubMed

Stanley, S O; Morita, R Y

1968-01-01

Salinity of the growth medium was found to have a marked effect on the maximal growth temperature of four bacteria isolated from marine sources. Vibrio marinus MP-1 had a maximal growth temperature of 21.2 C at a salinity of 35% and a maximal growth temperature of 10.5 C at a salinity of 7%, the lowest salinity at which it would grow. This effect was shown to be due to the presence of various cations in the medium. The order of effectiveness of cations in restoring the normal maximal growth temperature, when added to dilute seawater, was Na(+) > Li(+) > Mg(++) > K(+) > Rb(+) > NH(4) (+). The anions tested, with the exception of SO(4)=, had no marked effect on the maximal growth temperature response. In a completely defined medium, the highest maximal growth temperature was 20.0 C at 0.40 m NaCl. A decrease in the maximal growth temperature was observed at both low and high concentrations of NaCl.

Precipitation of solid phase calcium carbonates and their effect on application of seawater SA-T-P models

NASA Astrophysics Data System (ADS)

Marion, G. M.; Millero, F. J.; Feistel, R.

2009-07-01

At the present time, little is known about how broad salinity and temperature ranges are for seawater thermodynamic models that are functions of absolute salinity (SA), temperature (T) and pressure (P). Such models rely on fixed compositional ratios of the major components (e.g., Na/Cl, Mg/Cl, Ca/Cl, SO4/Cl, etc.). As seawater evaporates or freezes, solid phases [e.g., CaCO3(s) or CaSO42H2O(s)] will eventually precipitate. This will change the compositional ratios, and these salinity models will no longer be applicable. A future complicating factor is the lowering of seawater pH as the atmospheric partial pressures of CO2 increase. A geochemical model (FREZCHEM) was used to quantify the SA-T boundaries at P=0.1 MPa and the range of these boundaries for future atmospheric CO2 increases. An omega supersaturation model for CaCO3 minerals based on pseudo-homogeneous nucleation was extended from 25-40°C to 3°C. CaCO3 minerals were the boundary defining minerals (first to precipitate) between 3°C (at SA=104 g kg-) and 40°C (at SA=66 g kg-). At 2.82°C, calcite(CaCO3) transitioned to ikaite(CaCO36H2O) as the dominant boundary defining mineral for colder temperatures, which culminated in a low temperature boundary of -4.93°C. Increasing atmospheric CO2 from 385 μatm (390 MPa) (in Year 2008) to 550 μatm (557 MPa) (in Year 2100) would increase the SA and t boundaries as much as 11 g kg-1 and 0.66°C, respectively. The model-calculated calcite-ikaite transition temperature of 2.82°C is in excellent agreement with ikaite formation in natural environments that occurs at temperatures of 3°C or lower. Furthermore, these results provide a quantitative theoretical explanation (FREZCHEM model calculation) for why ikaite is the solid phase CaCO3 mineral that precipitates during seawater freezing.

Assessing spatial variability of soil properties and ions associated to salinity using the multifractal approach

NASA Astrophysics Data System (ADS)

Machado Siqueira, Glécio; Soares da Silva, Jucicleia; Farías França e Silva, Ênio; Lado, Marcos; Paz-González, Antonio; Vidal-Vázquez, Eva

2017-04-01

The lowlands coastal region of the state of Pernambuco, Northeast of Brazil, was formerly covered by humid Atlantic forest (Mata Atlântica) and then has been increasingly devoted to Sugar cane production. Because the water table is near to the soil surface salinity can occur in this area. The objective of this study was to assess the scale dependence of parameters associated to soil salinity and ions responsible for salination using multifractal analysis. The field work was conducted at an experimental field located in the Goiania municipality, Pernambuco, Brazil. This site is located 10 km east from the Atlantic coast. The field has been devoted to monoculture of sugarcane (Saccharum of?cinarum sp.) since 25 years. The climate of the region is tropical, with average annual temperature of 24°C and 1800 mm of precipitation per year. Soil was sampled every 3 m at 128 locations across a 384 m transect at a depth of 0-20 cm. The soil samples were analysed for pH, electrical conductivity (EC), Na+, K+, Ca2+, Mg2+, Cl- and SO4-2; also sodium adsorption ratio (SAR) was calculated. The spatial distributions of all the studied variables associated to soil salinity exhibited multifractal behaviour. Although all the variables studied exhibited a very strong power law scaling, different degrees of multifractality, assessed by differences in the amplitude and several selected parameters of the generalized dimension and singularity spectrum curves, have been appreciated. The multifractal approach gives a good description of the patterns of spatial variability of properties and ions describing soil salinity, and allows discriminating differences between them.

Slime coating of kaolinite on chalcopyrite in saline water flotation

NASA Astrophysics Data System (ADS)

Li, Zhi-li; Rao, Feng; Song, Shao-xian; Li, Yan-mei; Liu, Wen-biao

2018-05-01

In saline water flotation, the salinity can cause a distinguishable slime coating of clay minerals on chalcopyrite particles through its effect on their electrical double layers in aqueous solutions. In this work, kaolinite was used as a representative clay mineral for studying slime coating during chalcopyrite flotation. The flotation of chalcopyrite in the presence and absence of kaolinite in tap water, seawater, and gypsum-saturated water and the stability of chalcopyrite and kaolinite particles in slurries are presented. Zeta-potential distributions and scanning electron microscopy images were used to characterize and explain the different slime coating degrees and the different flotation performances. Kaolinite particles induced slime coating on chalcopyrite surfaces and reduced chalcopyrite floatability to the greatest extent when the pH value was in the alkaline range. At 0.24wt% of kaolinite, the chalcopyrite floatability was depressed by more than 10% at alkaline pH levels in tap water. Salinity in seawater and gypsum-saturated water compressed the electrical double layers and resulted in extensive slime coating.

Production and characterization of bioactive metabolites from piezotolerant deep sea fungus Nigrospora sp. in submerged fermentation.

PubMed

Arumugam, G K; Srinivasan, S K; Joshi, G; Gopal, D; Ramalingam, K

2015-01-01

To produce and characterize bioactive metabolites from piezotolerant marine fungus Nigrospora sp. in submerged fermentation. A distinct marine strain, Nigrospora sp. NIOT has been isolated from a depth of 800 m at the Arabian Sea. The 18S rRNA and internal transcribed spacers (ITS) analysis demonstrates its close association with the genus Nigrospora. Effect of pH, temperature, salinity, carbon source and amino acids was studied to optimize the fermentation conditions. Optimal mycelia growth and secondary metabolites production were observed at 6·0-8·0 pH, 20-30°C temperature, 7·5% salinity, sucrose as carbon source and tryptophan as amino acid source. The extracellular secondary metabolites exhibited high antimicrobial activities against both gram-positive and gram-negative pathogenic bacteria with minimal inhibitory concentration (MIC) values higher than 30 μg ml(-1). Strongest cytotoxicity was observed in all cell lines tested, GI50 (growth inhibition by 50%) was calculated to be 1·35, 3·2, 0·13 and 0·35 μg ml(-1) against U937, MCF-7, A673 and Jurkat, respectively. Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS) analyses of secondary metabolites confirmed the production of antimicrobial and anticancer substances. A piezotolerant fungus Nigrospora sp. NIOT isolated from deep sea environment was successfully cultured under submerged fermentation. The secondary metabolites produced from this organism showed potent antimicrobial and anticancer activities with immediate application to cosmetics and pharmaceutical industries. This is the first study exploring Nigrospora sp. from 800 m in marine environment. This deep sea fungus under optimized culture conditions effectively produced bioactive secondary metabolites such as griseofulvin, spirobenzofuran and pyrone derivatives at higher concentrations. © 2014 The Society for Applied Microbiology.

Halophilic microorganisms in deteriorated historic buildings: insights into their characteristics.

PubMed

Adamiak, Justyna; Otlewska, Anna; Gutarowska, Beata; Pietrzak, Anna

2016-01-01

Historic buildings are constantly being exposed to numerous climatic changes such as damp and rainwater. Water migration into and out of the material's pores can lead to salt precipitation and the so-called efflorescence. The structure of the material may be seriously threatened by salt crystallization. A huge pressure is produced when salt hydrates occupy larger spaces, which leads at the end to cracking, detachment and material loss. Halophilic microorganisms have the ability to adapt to high salinity because of the mechanisms of inorganic salt (KCl or NaCl) accumulation in their cells at concentrations isotonic to the environment, or compatible solutes uptake or synthesis. In this study, we focused our attention on the determination of optimal growth conditions of halophilic microorganisms isolated from historical buildings in terms of salinity, pH and temperature ranges, as well as biochemical properties and antagonistic abilities. Halophilic microorganisms studied in this paper could be categorized as a halotolerant group, as they grow in the absence of NaCl, as well as tolerate higher salt concentrations (Staphylococcus succinus, Virgibacillus halodenitrificans). Halophilic microorganisms have been also observed (Halobacillus styriensis, H. hunanensis, H. naozhouensis, H. litoralis, Marinococcus halophilus and yeast Sterigmatomyces halophilus). With respect to their physiological characteristics, cultivation at a temperature of 25-30°C, pH 6-7, NaCl concentration for halotolerant and halophilic microorganisms, 0-10% and 15-30%, respectively, provides the most convenient conditions. Halophiles described in this study displayed lipolytic, glycolytic and proteolytic activities. Staphylococcus succinus and Marinococcus halophilus showed strong antagonistic potential towards bacteria from the Bacillus genus, while Halobacillus litoralis displayed an inhibiting ability against other halophiles.

Estimation of palaeohydrochemical conditions using carbonate minerals

NASA Astrophysics Data System (ADS)

Amamiya, H.; Mizuno, T.; Iwatsuki, T.; Yuguchi, T.; Murakami, H.; Saito-Kokubu, Y.

2014-12-01

The long-term evolution of geochemical environment in deep underground is indispensable research subject for geological disposal of high-level radioactive waste, because the evolution of geochemical environment would impact migration behavior of radionuclides in deep underground. Many researchers have made efforts previously to elucidate the geochemical environment within the groundwater residence time based on the analysis of the actual groundwater. However, it is impossible to estimate the geochemical environment for the longer time scale than the groundwater residence time in this method. In this case, analysis of the chemical properties of secondary minerals are one of useful method to estimate the paleohydrochemical conditions (temperature, salinity, pH and redox potential). In particular, carbonate minerals would be available to infer the long-term evolution of hydrochemical for the following reasons; -it easily reaches chemical equilibrium with groundwater and precipitates in open space of water flowing path -it reflects the chemical and isotopic composition of groundwater at the time of crystallization We reviewed the previous studies on carbonate minerals and geochemical conditions in deep underground and estimated the hydrochemical characteristics of past groundwater by using carbonate minerals. As a result, it was found that temperature and salinity of the groundwater during crystallization of carbonate minerals were evaluated quantitatively. On the other hand, pH and redox potential can only be understood qualitatively. However, it is suggested that the content of heavy metal elements such as manganese, iron and uranium, and rare earth elements in the carbonate minerals are useful indicators for estimating redox potential. This study was carried out under a contract with METI (Ministry of Economy, Trade and Industry) as part of its R&D supporting program for developing geological disposal technology.

Antacid aspiration in rabbits: a comparison of Mylanta and Bicitra.

PubMed

Eyler, S W; Cullen, B F; Murphy, M E; Welch, W D

1982-03-01

The effects of aspiration of (a) 2 ml of Mylanta (a particulate antacid) mixed with 2 ml of hydrochloric acid (pH 1.5), (b) 2 ml of half-strength Bicitra (a soluble antacid) mixed with 2 ml of hydrochloric acid (pH 1.5), (c) 4 ml of hydrochloric acid (pH 1.5), and (d) 4 ml of normal saline (pH 6.5) on arterial blood gas tensions and lung pathology were compared in anesthetized rabbits. PaO2 decreased similarly in all animals 15 minutes after aspiration, but recovered to normal levels 4 hours after aspiration of saline and 48 hours after aspiration of Bicitra. PaO2 remained depressed after aspiration of Mylanta and HCl. Gross and microscopic evidence of lung injury was most severe in animals that aspirated Mylanta. One animal died 8 hours after aspiration of Mylanta.

Chemical Degradation of Polyacrylamide during Hydraulic Fracturing

NASA Astrophysics Data System (ADS)

Xiong, B.; Tasker, T.; Miller, Z.; Roman-White, S.; Farina, B.; Piechowicz, B.; Burgos, W.; Joshi, P.; Zhu, L.; Gorski, C.; Zydney, A.; Kumar, M.

2017-12-01

Polyacrylamide (PAM) based friction reducers are a primary ingredient of slickwater hydraulic fracturing fluids. Little is known regarding the fate of these polymers under downhole conditions, which could have important environmental impacts including strategies for reuse or treatment of flowback water. The objective of this study was to evaluate the chemical degradation of high molecular weight PAM, including the effects of shale, oxygen, temperature, pressure, and salinity. Data were obtained with a slickwater fracturing fluid exposed to both a shale sample collected from a Marcellus shale outcrop and to Marcellus core samples at high pressures/temperatures (HPT) simulating downhole conditions. Based on size exclusion chromatography analyses, the peak molecular weight of the PAM was reduced by two orders of magnitude, from roughly 10 MDa to 200 kDa under typical HPT fracturing conditions. The rate of degradation was independent of pressure and salinity but increased significantly at high temperatures and in the presence of oxygen dissolved in fracturing fluid. Results were consistent with a free radical chain scission mechanism, supported by measurements of sub-M hydroxyl radical concentrations. The shale sample adsorbed some PAM ( 30%), but importantly it catalyzed the chemical degradation of PAM, likely due to dissolution of Fe2+ at low pH. These results provide the first evidence of radical-induced degradation of PAM under HPT hydraulic fracturing conditions without additional oxidative breaker.

Chemical Degradation of Polyacrylamide during Hydraulic Fracturing.

PubMed

Xiong, Boya; Miller, Zachary; Roman-White, Selina; Tasker, Travis; Farina, Benjamin; Piechowicz, Bethany; Burgos, William D; Joshi, Prachi; Zhu, Liang; Gorski, Christopher A; Zydney, Andrew L; Kumar, Manish

2018-01-02

Polyacrylamide (PAM) based friction reducers are a primary ingredient of slickwater hydraulic fracturing fluids. Little is known regarding the fate of these polymers under downhole conditions, which could have important environmental impacts including decisions on strategies for reuse or treatment of flowback water. The objective of this study was to evaluate the chemical degradation of high molecular weight PAM, including the effects of shale, oxygen, temperature, pressure, and salinity. Data were obtained with a slickwater fracturing fluid exposed to both a shale sample collected from a Marcellus outcrop and to Marcellus core samples at high pressures/temperatures (HPT) simulating downhole conditions. Based on size exclusion chromatography analyses, the peak molecular weight of the PAM was reduced by 2 orders of magnitude, from roughly 10 MDa to 200 kDa under typical HPT fracturing conditions. The rate of degradation was independent of pressure and salinity but increased significantly at high temperatures and in the presence of oxygen dissolved in fracturing fluids. Results were consistent with a free radical chain scission mechanism, supported by measurements of sub-μM hydroxyl radical concentrations. The shale sample adsorbed some PAM (∼30%), but importantly it catalyzed the chemical degradation of PAM, likely due to dissolution of Fe 2+ at low pH. These results provide the first evidence of radical-induced degradation of PAM under HPT hydraulic fracturing conditions without additional oxidative breaker.

Physico-chemical parameters and Ichthyofauna diversity of Arasalar estuary in southeast coast of India

NASA Astrophysics Data System (ADS)

Raju, C.; Sridharan, G.; Mariappan, P.; Chelladurai, G.

2017-03-01

The physico-chemical changes may have the tendency to accumulate in the various organs of estuarine organisms, especially fish which may in turn enter into the human metabolism through consumption causing serious hazards. Hence, the present study was carried out to dete rmine the physico-chemical characteristics of water and Ichthyofauna in Arasalar estuary in southeast coast of India for the period of 1 year during September 2012-August 2013. The environmental parameters such as, temperature, pH, salinity, DO, silicate, nitrate and phosphate were observed from Department of Zoology, Rajah Serfoji Goverment College, Thanjavur, Tamil Nadu, India. During the period of study, air temperature varied from 28.8 to 35 °C. The surface water temperature also varied from 25 to 31.5 °C. The monthly mean values of hydrogen ion concentration of water varied from 7.1 to 8.2. The salinity of water varied from 5.5 ‰ to 34. Dissolved oxygen in Arasalar estuary was varied from 3.5 to 7.2 mg/l. The total phosphorus varied from 0.29 to 2.15 µg/1. The nitrate varied from 0.47 to 3.75 µg/l. The silicate content varied from 28.25 to 98.74 µg/l. Totally 866 fishes were collected belonging to 4 orders and 5 families. Mystus gulio was found to be the dominant species (25.40 %) in the study area.

Sea-surface temperature and salinity mapping from remote microwave radiometric measurements of brightness temperature

NASA Technical Reports Server (NTRS)

Hans-Juergen, C. B.; Kendall, B. M.; Fedors, J. C.

1977-01-01

A technique to measure remotely sea surface temperature and salinity was demonstrated with a dual frequency microwave radiometer system. Accuracies in temperature of 1 C and in salinity of part thousand for salinity greater than 5 parts per thousand were attained after correcting for the influence of extraterrestrial background radiation, atmospheric radiation and attenuation, sea-surface roughness, and antenna beamwidth. The radiometers, operating at 1.43 and 2.65 GHz, comprise a third-generation system using null balancing and feedback noise injection. Flight measurements from an aircraft at an altitude of 1.4 km over the lower Chesapeake Bay and coastal areas of the Atlantic Ocean resulted in contour maps of sea-surface temperature and salinity with a spatial resolution of 0.5 km.

Influence of environmental variables in the efficiency of phage therapy in aquaculture.

PubMed

Silva, Yolanda J; Costa, Liliana; Pereira, Carla; Cunha, Ângela; Calado, Ricardo; Gomes, Newton C M; Almeida, Adelaide

2014-09-01

Aquaculture facilities worldwide continue to experience significant economic losses because of disease caused by pathogenic bacteria, including multidrug-resistant strains. This scenario drives the search for alternative methods to inactivate pathogenic bacteria. Phage therapy is currently considered as a viable alternative to antibiotics for inactivation of bacterial pathogens in aquaculture systems. While phage therapy appears to represent a useful and flexible tool for microbiological decontamination of aquaculture effluents, the effect of physical and chemical properties of culture waters on the efficiency of this technology has never been reported. The present study aimed to evaluate the effect of physical and chemical properties of aquaculture waters (e.g. pH, temperature, salinity and organic matter content) on the efficiency of phage therapy under controlled experimental conditions in order to provide a basis for the selection of the most suitable protocol for subsequent experiments. A bioluminescent genetically transformed Escherichia coli was selected as a model microorganism to monitor real-time phage therapy kinetics through the measurement of bioluminescence, thus avoiding the laborious and time-consuming conventional method of counting colony-forming units (CFU). For all experiments, a bacterial concentration of ≈ 10(5) CFU ml(-1) and a phage concentration of ≈ 10(6-8) plaque forming unit ml(-1) were used. Phage survival was not significantly affected by the natural variability of pH (6.5-7.4), temperature (10-25 °C), salinity (0-30 g NaCl l(-1) ) and organic matter concentration of aquaculture waters in a temperate climate. Nonetheless, the efficiency of phage therapy was mostly affected by the variation of salinity and organic matter content. As the effectiveness of phage therapy increases with water salt content, this approach appears to be a suitable choice for marine aquaculture systems. The success of phage therapy may also be enhanced in non-marine systems through the addition of salt, whenever this option is feasible and does not affect the survival of aquatic species being cultured. © 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

Hemolysis and cytotoxicity mechanisms of biodegradable magnesium and its alloys.

PubMed

Zhen, Zhen; Liu, Xiaoli; Huang, Tao; Xi, TingFei; Zheng, Yufeng

2015-01-01

Good hemocompatibility and cell compatibility are essential requirements for coronary stents, especially for biodegradable magnesium alloy stents, which could change the in situ environment after implanted. In this work, the effects of magnesium ion concentration and pH value on the hemolysis and cytotoxicity have been evaluated. Solution with different Mg(2+) concentration gradients and pH values of normal saline and cell culture media DMEM adjusted by MgCl2 and NaOH respectively were tested for the hemolysis and cell viability. Results show that even when the concentration of Mg(2+) reaches 1000 μg/mL, it has little destructive effect on erythrocyte, and the high pH value over 11 caused by the degradation is the real reason for the high hemolysis ratio. Low concentrations of Mg(2+) (<100 μg/mL) cause no cytotoxicity to L929 cells, of which the cell viability is above 80%, while high concentrations of Mg(2+) (>300 μg/mL) could induce obvious death of the L929 cells. The pH of the extract plays a synergetic effect on cytotoxicity, due to the buffer action of the cell culture medium. To validate this conclusion, commercial pure Mg using normal saline and PBS as extract was tested with the measurement of pH and Mg(2+) concentration. Pure Mg leads to a higher hemolysis ratio in normal saline (47.76%) than in buffered solution (4.38%) with different pH values and low concentration of Mg(2+). The Mg extract culture media caused no cytotoxicity, with pH=8.44 and 47.80 μg/mL Mg(2+). It is suggested that buffered solution and dynamic condition should be adopted in the hemolysis evaluation. Copyright © 2014. Published by Elsevier B.V.

Effect of proton pump inhibitors on gastric juice volume, gastric pH and gastric intramucosal pH in critically ill patients : a randomized, double-blind, placebo-controlled study.

PubMed

Gursoy, Olcay; Memiş, Dilek; Sut, Necdet

2008-01-01

This study aimed to determine the effect of administration of a single-dose proton pump inhibitor (PPI) on gastric intramucosal pH (pHi), gastric juice volume and gastric pH in critically ill patients. This prospective, randomized, double-blind, placebo-controlled study included 75 patients who were divided into five groups that received the following treatment: group C (n = 15), saline 100 mL; group O (n = 15), omeprazole 20 mg; group P (n = 15), pantoprazole 40 mg; group E (n = 15), esomeprazole 20 mg; and group R (n = 15), rabeprazole 20 mg. All treatments were administered nasogastrically in 100 mL of physiological saline. Measurements of gastric pHi, gastric juice volume and gastric pH were obtained immediately before and 2, 4 and 6 hours after administration of treatments. In addition, gastric content was aspirated and its volume was recorded. Initial gastric pHi, gastric juice volume and gastric pH values were not statistically significantly different among the groups (p > 0.05). No statistically significant difference in gastric pHi was seen among the groups before or 2, 4 or 6 hours after saline or PPI administration. At hours 2, 4 and 6, gastric pH in the pantoprazole, esomeprazole and rabeprazole groups increased significantly, whereas gastric juice volume decreased significantly, compared with the omeprazole and placebo groups (p < 0.001). No statistically significant differences were seen between the pantoprazole, esomeprazole and rabeprazole groups. This is the first study to show that single-dose pantoprazole, esomeprazole and rabeprazole are associated with greater gastric pH increase and greater gastric juice volume decrease than omeprazole in critically ill patients. Our study also suggests that PPIs do not affect gastric pHi measurements in critically ill patients and can be administered during pH monitoring.

Salting-out assisted liquid-liquid extraction combined with gas chromatography-mass spectrometry for the determination of pyrethroid insecticides in high salinity and biological samples.

PubMed

Niu, Zongliang; Yu, Chunwei; He, Xiaowen; Zhang, Jun; Wen, Yingying

2017-09-05

A salting-out assisted liquid-liquid extraction (SALLE) combined with gas chromatography-mass spectrometry (GC-MS) method was developed for the determination of four pyrethroid insecticides (PYRs) in high salinity and biological samples. Several parameters including sample pH, salting-out solution volume and salting-out solution pH influencing the extraction efficiency were systematically investigated with the aid of orthogonal design. The optimal extraction conditions of SALLE were: 4mL of salting-out solution with pH=4 and the sample pH=3. Under the optimum extraction and determination conditions, good responses for four PYRs were obtained in a range of 5-5000ng/mL, with linear coefficients greater than 0.998. The recoveries of the four PYRs ranged from 74% to 110%, with standard deviations ranging from 1.8% to 9.8%. The limits of detection based on a signal-to-noise ratio of 3 were between 1.5-60.6ng/mL. The method was applied to the determination of PYRs in urine, seawater and wastewater samples with a satisfactory result. The results demonstrated that this SALLE-GC-MS method was successfully applied to determine PYRs in high salinity and biological samples. SALLE avoided the need for the elimination of salinity and protein in the sample matrix, as well as clean-up of the extractant. Most of all, no centrifugation or any special apparatus are required, make this a promising method for rapid sample preparation procedure. Copyright © 2017 Elsevier B.V. All rights reserved.

Resilience of sulfate-reducing granular sludge against temperature, pH, oxygen, nitrite, and free nitrous acid.

PubMed

Hao, Tianwei; Mackey, Hamish R; Guo, Gang; Liu, Rulong; Chen, Guanghao

2016-10-01

Sulfate-reducing granular sludge has recently been developed and characterized in detail as part of the development of the sulfate reduction, autotrophic denitrification, nitrification integrated (SANI) process. However, information regarding temperature of granules to environmental fluctuation is lacking, an aspect that is important in dealing with real wastewater. A comprehensive assessment of sulfate-reducing granular sludge performance under various environmental conditions was thus conducted in this study, including temperature, pH, oxygen, nitrite, and free nitrous acid (FNA) as possible encountering conditions in the removal of organics and/or nitrate. Specific chemical oxygen demand removal rate of the granules was determined to be reduced by 65 % when the temperature varied between 10-15 °C, reduced by 70 % when dissolved oxygen (DO) was 0.5 mg/L or greater, and at least, reduced by 75 % when nitrite was 30 mg N/L or above. Nevertheless, the sludge activity recovered by 82, 100, and 86 % from exposure to high oxygen and nitrite and low temperature levels, respectively. Combined inhibition of nitrite and FNA on the sludge is strong and complex, while FNA alone reduced cell viability from 60 to 40 % when its concentration increased to 2.3 mg N/L. The present study demonstrates that sulfate-reducing bacteria (SRB) granules possess high resilience against varying environmental conditions, showing the high application potential of sulfate-reducing granular sludge in dealing with brackish and saline industrial or domestic wastewaters.

Random Forest Segregation of Drug Responses May Define Regions of Biological Significance

PubMed Central

Bukhari, Qasim; Borsook, David; Rudin, Markus; Becerra, Lino

2016-01-01

The ability to assess brain responses in unsupervised manner based on fMRI measure has remained a challenge. Here we have applied the Random Forest (RF) method to detect differences in the pharmacological MRI (phMRI) response in rats to treatment with an analgesic drug (buprenorphine) as compared to control (saline). Three groups of animals were studied: two groups treated with different doses of the opioid buprenorphine, low (LD), and high dose (HD), and one receiving saline. PhMRI responses were evaluated in 45 brain regions and RF analysis was applied to allocate rats to the individual treatment groups. RF analysis was able to identify drug effects based on differential phMRI responses in the hippocampus, amygdala, nucleus accumbens, superior colliculus, and the lateral and posterior thalamus for drug vs. saline. These structures have high levels of mu opioid receptors. In addition these regions are involved in aversive signaling, which is inhibited by mu opioids. The results demonstrate that buprenorphine mediated phMRI responses comprise characteristic features that allow a supervised differentiation from placebo treated rats as well as the proper allocation to the respective drug dose group using the RF method, a method that has been successfully applied in clinical studies. PMID:27014046

Capsaicin pre- and post-treatment on rat monocrotaline pneumotoxicity.

PubMed

Katzman, N J; Lai, Y L

2000-12-31

Monocrotaline (MCT) produces respiratory dysfunction, pulmonary hypertension (PH), and right ventricular hypertrophy (RVH) in rats. Tachykinins, such as substance P (SP) and neurokinin A (NKA), may mediate these effects. The purpose of this study was to investigate the length of tachykinin depletion (via capsaicin treatment) is needed to prevent (or attenuate) PH and/or RVH. Six groups of rats were injected subcutaneously with saline (3 ml/kg); capsaicin followed by saline or MCT (60 mg/kg); or MCT followed 7, 11, or 14 days later by capsaicin. Capsaicin (cumulative dose, 500 mg/kg) was given over a period of 4-5 days. Respiratory function, pulmonary vascular parameters, lung tachykinin levels, and tracheal neutral endopeptidase (NEP) activity were measured 21 days after MCT or saline injection. Capsaicin significantly decreased lung levels of SP but not NKA. Both capsaicin pretreatment and posttreatment blocked the following MCT-induced alterations: increases in lung SP and airway constriction; decreases in tracheal NEP activity and dynamic respiratory compliance. Administration of capsaicin before or 7 days after MCT blocked MCT-induced PH and RVH. The above data suggest that the early tachykinin-mediated airway dysfunction requires only transient elevated tachykinins, while progression of late tachykinin-mediated effects (PH and RVH) requires elevated tachykinins for more than one week.

Effects of temperature and Ca2+ on the larval development of the decapoda crustacean: Eriocheir sinensis

NASA Astrophysics Data System (ADS)

Huang, Bo; Du, Nan-Shan; Lai, Wei

2001-09-01

With increasing demand in China for the mitten crab larvae, understanding its survival mechanism gets more important. This research focused on the effects of temperature and Ca2+ on the larval growth and development. Eriocheir sinensis larvae were reared in laboratory under, 21 different combinations of temperature (15, 20, 25°C) and Ca2+ content (120, 130, 140, 150, 160, 170, 180 mg/l) and constant salinity (20) and pH (8). The results suggested that the survival rate increases with temperature and Ca2+ content. These combinations of temperature and Ca2+ content maximized survival rate in our study and it may be the optimum water environmental conditions for culturing, the larvae. To predict surviving larvae number under different water environmental conditions, 21 dynamic mathematical models were developed. This for the first time observation of the zoeal VI larvae of the Changjiang River E. sinesis population showed that they occurred under stressed water environmental conditions: temperature of 15°C and Ca2+ content of 120, 130 mg/l.

Species Profiles. Life Histories and Environmental Requirements of Coastal Fishes and Invertebrates (Pacific Southwest) Crangonid Shrimp

DTIC Science & Technology

1989-12-01

temperature affected survival at different shelter has been suggested as an important salinities and salinity affected survival at mechanism of nutrient...10 Salinity . . . . .. . . . . .. . . . .. . .. . .. . .. .. . .. .. .. .. . .. . . .. . .. . . . . .. . . . 11 Temperature- Salinity ...members of the tribe Caridca by four features Order .................... Decapoda (Figure 3); (1)-the rostrum is very short, Suborder

Oscillating Adriatic temperature and salinity regimes mapped using the Self-Organizing Maps method

NASA Astrophysics Data System (ADS)

Matić, Frano; Kovač, Žarko; Vilibić, Ivica; Mihanović, Hrvoje; Morović, Mira; Grbec, Branka; Leder, Nenad; Džoić, Tomislav

2017-01-01

This paper aims to document salinity and temperature regimes in the middle and south Adriatic Sea by applying the Self-Organizing Maps (SOM) method to the available long-term temperature and salinity series. The data were collected on a seasonal basis between 1963 and 2011 in two dense water collecting depressions, Jabuka Pit and Southern Adriatic Pit, and over the Palagruža Sill. Seasonality was removed prior to the analyses. Salinity regimes have been found to oscillate rapidly between low-salinity and high-salinity SOM solutions, ascribed to the advection of Western and Eastern Mediterranean waters, respectively. Transient salinity regimes normally lasted less than a season, while temperature transient regimes lasted longer. Salinity regimes prolonged their duration after the major basin-wide event, the Eastern Mediterranean Transient, in the early 1990s. A qualitative relationship between high-salinity regimes and dense water formation and dynamics has been documented. The SOM-based analyses have a large capacity for classifying the oscillating ocean regimes in a basin, which, in the case of the Adriatic Sea, beside climate forcing, is an important driver of biogeochemical changes that impacts trophic relations, appearance and abundance of alien organisms, and fisheries, etc.

Estimation of Geotropic Currents in the Bay of Bengal using In-situ Observations.

NASA Astrophysics Data System (ADS)

T, V. R.

2014-12-01

Geostraphic Currents (GCs) can be estimated from temperature and salinity observations. In this study an attempt has been made to compute GC using temperature and salinity observations from Expendable Bathy Thermograph (XBT) and CTD over Bay of Bengal (BoB). Although in recent time we have Argo observations but it is for a limited period and coarse temporal resolutions. In BoB Bengal, where not enough simultaneous hydrographic temperature and salinity data are available with reasonable spatial resolution (~one degree spatial resolution) and for a longer period. To overcome the limitations of GC computed from XBT profiles, temperature-salinity relationships were used from simultaneous temperature and salinity observations. We have demonstrated that GCs can be computed with an accuracy of less than 8.5 cm/s (root mean square error) at the surface with respect to temperature from XBT and salinity from climatological record. This error reduces with increasing depth. Finally, we demonstrated the application of this approach to study the temporal variation of the GCs during 1992 to 2012 along an XBT transect.

Hydrochemistry of the Tumen River Estuary, Sea of Japan

NASA Astrophysics Data System (ADS)

Tishchenko, P. Ya.; Semkin, P. Yu.; Pavlova, G. Yu.; Tishchenko, P. P.; Lobanov, V. B.; Marjash, A. A.; Mikhailik, T. A.; Sagalaev, S. G.; Sergeev, A. F.; Tibenko, E. Yu.; Khodorenko, N. D.; Chichkin, R. V.; Shvetsova, M. G.; Shkirnikova, E. M.

2018-03-01

The hydrological and hydrochemical parameters of the Tumen River estuary were collected at 13 stations in May and October 2015. Vertical temperature, conductivity, dissolved oxygen, chlorophyll fluorescence, and turbidity profiles were obtained. Water was sampled from the surface and bottom layer. The water samples were analyzed for major ions, pH, salinity, concentrations of dissolved oxygen, major nutrients, dissolved organic carbon, humic matter, and δ18O and δD isotopes. This estuary is attributed to microtidal type with a flushing time of about 10 h. A phytoplakton bloom occurred in the top layer of the estuary. For surface horizons, the hydrochemical parameters show a linear correlation with salinity. In the bottom horizons, all these parameters, except for major ions and δ18O and δD isotopes, reveal substantial nonconservative behavior. The nonconservative behavior of the hydrochemical parameters in the bottom waters was mainly caused by degradation of the phytoplankton biomass at the water/sediment interface. Hypoxic conditions were established in the bottom waters of the estuary in May.

Climate change impacts on marine water quality: The case study of the Northern Adriatic sea.

PubMed

Rizzi, J; Torresan, S; Critto, A; Zabeo, A; Brigolin, D; Carniel, S; Pastres, R; Marcomini, A

2016-01-30

Climate change is posing additional pressures on coastal ecosystems due to variations in water biogeochemical and physico-chemical parameters (e.g., pH, salinity) leading to aquatic ecosystem degradation. With the main aim of analyzing the potential impacts of climate change on marine water quality, a Regional Risk Assessment methodology was developed and applied to coastal marine waters of the North Adriatic. It integrates the outputs of regional biogeochemical and physico-chemical models considering future climate change scenarios (i.e., years 2070 and 2100) with site-specific environmental and socio-economic indicators. Results showed that salinity and temperature will be the main drivers of changes, together with macronutrients, especially in the area of the Po' river delta. The final outputs are exposure, susceptibility and risk maps supporting the communication of the potential consequences of climate change on water quality to decision makers and stakeholders and provide a basis for the definition of adaptation and management strategies. Copyright © 2015 Elsevier Ltd. All rights reserved.

Spatial variability of E. coli in an urban salt-wedge estuary.

PubMed

Jovanovic, Dusan; Coleman, Rhys; Deletic, Ana; McCarthy, David

2017-01-15

This study investigated the spatial variability of a common faecal indicator organism, Escherichia coli, in an urban salt-wedge estuary in Melbourne, Australia. Data were collected through comprehensive depth profiling in the water column at four sites and included measurements of temperature, salinity, pH, dissolved oxygen, turbidity, and E. coli concentrations. Vertical variability of E. coli was closely related to the salt-wedge dynamics; in the presence of a salt-wedge, there was a significant decrease in E. coli concentrations with depth. Transverse variability was low and was most likely dwarfed by the analytical uncertainties of E. coli measurements. Longitudinal variability was also low, potentially reflecting minimal die-off, settling, and additional inputs entering along the estuary. These results were supported by a simple mixing model that predicted E. coli concentrations based on salinity measurements. Additionally, an assessment of a sentinel monitoring station suggested routine monitoring locations may produce conservative estimates of E. coli concentrations in stratified estuaries. Copyright © 2016 Elsevier Ltd. All rights reserved.

Anomalous chemical changes in well waters and possible relation to earthquakes

NASA Astrophysics Data System (ADS)

King, Chi-Yu; Evans, William C.; Presser, Theresa; Husk, Robert H.

Water level, temperature, salinity, electric conductivity, and pH have been measured periodically for several years at three water wells located along a 17-km segment of the San Andreas fault between San Juan Bautista and Cienega Winery in central California. Water samples were collected at the same time for subsequent chemical analyses in the laboratory. Some sudden large changes in salinity and conductivity were recorded in early March 1980 at the two wells near San Juan Bautista. These changes coincided approximately with the beginning of an episode of increased local seismicity, including a magnitude 4.8 earthquake on April 13. Analyses of water samples revealed corresponding changes in ion concentrations, especially of Na+, Ca++, Mg++, SO4--, HCO3-, F-, and Cl-. The observed changes may be the result of mixing of waters from different aquifers through cracks developed in the water barriers by a possible crustal strain episode that may have occurred in the study area.

Anomalous chemical changes in well waters and possible relation to earthquakes.

USGS Publications Warehouse

Chi-Yu, King; Evans, William C.; Presser, T.; Husk, R.H.

1981-01-01

Water level, temperature, salinity, electric conductivity, and pH have been measured periodically for several years at three water wells located along a 17km segment of the San Andreas fault between San Juan Bautista and Cienega Winery in central California. Water samples were collected at the same time for subsequent chemical analyses in the laboratory. Some sudden large changes in salinity and conductivity were recorded in early March 1980 at the two wells near San Juan Bautista. These changes coincided approximately with the beginning of an episode of increased local seismicity, including a magnitude 4.8 earthquake on April 13. Analyses of water samples revealed corresponding changes in ion concentrations, especially of Na+, Ca2+, Mg2+, 035SO42-, 046HCO3-, F-, and Cl-. The observed changes may be the result of mixing of waters from different aquifers through cracks developed in the water barriers by a possible crustal strain episode that may have occurred in the study area.-Authors

Characterization of protease and effects of temperature and salinity on the biochemical changes during fermentation of Antarctic krill.

PubMed

Fan, Yan; Tian, Lili; Xue, Yong; Li, Zhaojie; Hou, Hu; Xue, Changhu

2017-08-01

Despite their abundance, Antarctic krill are underutilized because of numerous difficulties in their commercial processing. Ideally, fermentation technology can be applied to transform them into a popular condiment. In addition to the exploration of protease properties, the present study aimed to evaluate proteinase activity, pH, amino nitrogen, and histamine formation during fermentation at different temperatures and salt treatments. Even though the activity of Antarctic krill protease reached a maximum at 40 °C and pH 7, it was stable at 30 °C and pH 7-9. Among the metal ions tested, Ca 2+ , Mg 2+ and K + increased protease activity, in contrast to Zn 2+ and Cu 2+ . Within each treatment, the highest protease activity and amino nitrogen content, as well as the lowest histamine level, were observed on day 12 of fermentation. Treatment at 35 °C with 180 g kg -1 salt led to the production of maximum amino nitrogen (0.0352 g kg -1 ) and low histamine (≤0.0497 g kg -1 ). Krill paste fermented for 12 days at 35 °C with 180 g kg -1 salt exhibited the optimal quality and properties, suggesting an efficient method for fermentation of Antarctic krill and other aquatic resources. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

The role of Te(IV) and Bi(III) chloride complexes in hydrothermal mass transfer: An X-ray absorption spectroscopic study

DOE Office of Scientific and Technical Information (OSTI.GOV)

Etschmann, Barbara E.; Liu, Weihua; Pring, Allan

2016-05-01

Tellurium (Te) and bismuth (Bi) are two metal(loid)s often enriched together with gold (Au) in hydrothermal deposits; however the speciation and transport properties for these two metals in hydrothermal systems are poorly understood. We investigated the effect of chloride on the speciation of Te(IV) and Bi(III) in hydrothermal solutions using in-situ XAS spectroscopy. At ambient temperature, oxy-hydroxide complexes containing the [TeO3] moiety (e.g., H3TeO3+ under highly acidic conditions) predominate in salty solutions over a wide range in pH and salt concentrations. Te(IV)-Cl complexes only appear at pH(25 degrees C) <= 2 and high Cl- activity (>= 10). The highest ordermore » Te(IV) chloride complex detected is TeCl4(aq), and contains the [TeCl4] moiety. Upon heating to 199 degrees C, the Te(IV)-Cl complexes become more stable; however they still required highly acidic conditions which are likely to exist only in very limited environments in nature. At ambient temperature, Bi(III) is coordinated to 5.5(5) Cl atoms in high salinity, acidic (HCl >= 0.5 m) chloride solutions. This, combined with large EXAFS-derived structural disorder parameters, suggests that the Bi(III) complex is most likely present as both BiCl52- and BiCl63-. The number of Cl atoms coordinated to Bi(III) decreases with increasing temperature; at around 200 degrees C and above, Bi(III) is coordinated to three Cl atoms. Overall the data show that Te(IV) chloride complexes can be ignored in predicting Te mobility under oxidizing conditions in most geological environments, but that Bi(III) chloride complexes are expected to account for Bi mobility in acidic brines. New thermodynamic properties for Bi(III) chloride complexes are provided to improve reactive transport modeling of Bi up to 500 degrees C. Although higher order complexes such as BiCl52- and BiCl63- exist at ambient temperature, the BiCl3(aq) complex becomes the predominant chloride complex in saline solutions at T >= 200 degrees C.« less

Biochemical characteristics and modulation by external and internal factors of aminopeptidase-N activity in the hepatopancreas of a euryhaline burrowing crab.

PubMed

Michiels, M S; del Valle, J C; López Mañanes, A A

2015-07-01

Strikingly, in spite of its physiological importance, information about occurrence, biochemical characteristics and mechanisms of regulation of aminopeptidase-N (APN) in the hepatopancreas of intertidal euryhaline crabs is still lacking. In this work, we determined the occurrence, biochemical characteristics, response to environmental salinity and dopamine of APN in the hepatopancreas of the euryhaline crab Neohelice granulata (Dana 1851) from the open mudflat of Mar Chiquita coastal lagoon (Buenos Aires province, Argentina). APN activity was maximal at pH and temperature range of 7.6-9.0 and 37-45 °C, respectively. APN activity exhibited Michaelis-Menten kinetics (apparent Km = 0.19 ± 0.10 mM) (pH 7.6, 37 °C) and appeared to be sensitive to bestatin (I 50 = 15 mM) and EDTA (I 50 = 9 mM). In crabs acclimated to 10 psu (hyper-regulation conditions) and 37 psu (hypo-regulation conditions), APN activity was about 45 and 160% higher, respectively, than in 35 psu (osmoconformation). APN activity in the hepatopancreas was stimulated in vitro (about 137%) by 10(-4) M dopamine. Higher dopamine concentrations produced a similar extent of increase. The responses of APN activity to salinity and dopamine in vitro suggest the role of APN in digestive adjustments upon hyper and hypo-regulatory conditions and its modulation via direct mechanisms on hepatopancreas by dopamine.

In vitro analysis of the physical properties of contact lens blister pack solutions.

PubMed

Menzies, Kara L; Jones, Lyndon

2011-04-01

Since the initial development of silicone hydrogels, many modifications to the bulk and surface properties of the lenses have been undertaken to improve the wettability and comfort of the lenses. Recently, manufacturers have incorporated various "wetting agents" or surface-active agents into the blister packaging solutions (BPSs) of the lenses to improve initial comfort of the lens on eye. The purpose of this study was to measure and compare the pH, surface tension (ST), viscosity, and osmolality of BPSs for a variety of silicone hydrogel and polyHEMA-based hydrogel lenses. In addition, two saline solutions were tested for comparison purposes. The pH, osmolality, ST, and viscosity were measured for the BPSs for lotrafilcon B and lotrafilcon A and lotrafilcon B with a "modified BPS" (m-lotrafilcon A, m-lotrafilcon B) (CIBA Vision, Duluth, GA); balafilcon A (Bausch & Lomb, Rochester, NY); galyfilcon A, senofilcon A, and narafilcon A (Johnson & Johnson, Jacksonville, FL); and comfilcon A and enfilcon A (CooperVision, Pleasanton, CA) and BPSs from two conventional polyHEMA-based materials-etafilcon A (Johnson & Johnson) and omafilcon A (CooperVision). The two saline solutions tested were Unisol (Alcon, Fort Worth, TX) and Softwear Saline (CIBA Vision). The pH results for the two saline solutions and all BPSs remained in the pH range of tears (6.6-7.8). The ST of the modified BPS was significantly lower (p < 0.01) than the original non-modified BPS. Viscosity measurements ranged between 0.90 and 1.00 cP for all BPSs and saline solutions, except for the modified BPS, which had significantly higher viscosities (p < 0.001). Osmolality measurements were not significantly different (p > 0.05) between BPSs made by the same manufacturer but were significantly different compared with BPSs made by different manufacturers (p < 0.05). The incorporation of wetting agents and surfactants into BPSs does alter the physical properties of the BPSs, which may have clinical implications regarding initial in-eye comfort.

The importance of pH and sand substrate in the revegetation of saline non-waterlogged peat fields.

PubMed

Montemayor, Marilou B; Price, Jonathan; Rochefort, Line

2015-11-01

A partially peat-extracted coastal bog contaminated by seawater was barren and required revegetation as a wetland. Peat fields were rectangular in shape, cambered in cross-section profile, and separated by drainage ditches. Common to all peat fields were symmetrical patterns in micro-topography with slopes between differences in elevation. Saline non-waterlogged slopes of ∼5% occurred as a symmetrical pair on each side of the crest of the cambered profile, at one end of each peat field. Three rows were laid across this slope (Top, Middle, and Bottom rows) and transplanted with naturally-growing plant species with their sand substrate, in three experiments, and grown for a year. In the Spartina pectinata experiment, bare root stem sections were also planted. Another experiment was conducted to determine changes in the characteristics of a volume of sand when incubated in saline peat fields. We found the salinity of peat increased with moisture downslope, and pH decreased with increase in salinity. S. pectinata grew best when planted with its sand substrate compared with bare root stem section, and when planted in Bottom rows. Juncus balticus had excellent growth in all rows. Unexpectedly, Festuca rubra that was inconspicuous beneath the J. balticus canopy in the natural donor site grew densely within the J. balticus sods. Agrostis stolonifera grew well but seemed to show intolerance to the surrounding acidic peat by curling up its stolons. The pH of the incubated sand volume was much higher than the surrounding peat. These studies suggest that recognition of plant niches and pH manipulation are important in the revegetation of disturbed Sphagnum peatlands that are found abundantly in the northern hemisphere. Results are also relevant to the reclamation of other disturbed lands. Copyright © 2015 Elsevier Ltd. All rights reserved.

Infrared thermal remote sensing for soil salinity assessment on landscape scale

NASA Astrophysics Data System (ADS)

Ivushkin, Konstantin; Bartholomeus, Harm; Bregt, Arnold K.; Pulatov, Alim; Bui, Elisabeth N.; Wilford, John

2017-04-01

Soil salinity is considered as one of the most severe land degradation aspects. An increased soil salt level inhibits growth and development of crops. Therefore, up to date soil salinity information is vital for appropriate management practices and reclamation strategies. This information is required at increasing spatial and temporal resolution for appropriate management adaptations. Conventional soil sampling and associated laboratory analyses are slow, expensive, and often cannot deliver the temporal and spatial resolution required. The change of canopy temperature is one of the stress indicators in plants. Its behaviour in response to salt stress on individual plant level is well studied in laboratory and greenhouse experiments, but its potential for landscape scale studies using remote sensing techniques is not investigated yet. In our study, possibilities of satellite thermography for landscape scale soil salinity assessment of cropped areas were studied. The performance of satellite thermography is compared with other approaches that have been used before, like Normalised Difference Vegetation Index (NDVI) and Enhanced Vegetation Index (EVI). The study areas were Syrdarya province of Uzbekistan and four study areas in four Australian states namely, Western Australia, South Australia, Queensland and New South Wales. The diversity of the study areas allowed us to analyse behaviour of canopy temperature of different crops (wheat, cotton, barley) and different agriculture practices (rain fed and irrigated). MODIS and Landsat TM multiannual satellite images were used to measure canopy temperature. As ground truth for Uzbekistan study area we used a provincial soil salinity map. For the Australian study areas we used the EC map for the whole country. ANOVA was used to analyse relations between the soil salinity maps and canopy temperature, NDVI, EVI. Time series graphs were created to analyse the dynamics of the indicators during the growing season. The results showed significant relations between the soil salinity maps and canopy temperature. The amplitude of canopy temperature difference between salinity classes varies for different crops, but the trend of temperature increase under increased salinity is present in all cases. The calculated F-values were higher for canopy temperature than for all other compared indicators. The vegetation indices also showed significant differences, but F-values were lower compared to canopy temperature. Also the visual comparison of the soil salinity map and the canopy temperature map show similar spatial patterns. The NDVI and EVI maps look more random and noisy and patterns are less pronounced than for the canopy temperature map. The strongest relation between the soil salinity map and canopy temperature was usually observed at the end of a dry season and in the period of maximum crop development. Satellite thermography appeared to be a valuable approach to detect soil salinity under agricultural crops at landscape scale.

Continuous salinity and temperature data from san francisco estuary, 19822002: Trends and the salinity-freshwater inflow relationship

USGS Publications Warehouse

Shellenbarger, G.G.; Schoellhamer, D.H.

2011-01-01

The U.S. Geological Survey and other federal and state agencies have been collecting continuous temperature and salinity data, two critical estuarine habitat variables, throughout San Francisco estuary for over two decades. Although this dynamic, highly variable system has been well studied, many questions remain relating to the effects of freshwater inflow and other physical and biological linkages. This study examines up to 20 years of publically available, continuous temperature and salinity data from 10 different San Francisco Bay stations to identify trends in temperature and salinity and quantify the salinityfreshwater inflow relationship. Several trends in the salinity and temperature records were identified, although the high degree of daily and interannual variability confounds the analysis. In addition, freshwater inflow to the estuary has a range of effects on salinity from -0.0020 to -0.0096 (m3 s-1) -1 discharge, depending on location in the estuary and the timescale of analyzed data. Finally, we documented that changes in freshwater inflow to the estuary that are within the range of typical management actions can affect bay-wide salinities by 0.61.4. This study reinforces the idea that multidecadal records are needed to identify trends from decadal changes in water management and climate and, therefore, are extremely valuable. ?? 2011 Coastal Education & Research Foundation.

Plasma osmolality and oxygen consumption of perch Perca fluviatilis in response to different salinities and temperatures.

PubMed

Christensen, E A F; Svendsen, M B S; Steffensen, J F

2017-03-01

The present study determined the blood plasma osmolality and oxygen consumption of the perch Perca fluviatilis at different salinities (0, 10 and 15) and temperatures (5, 10 and 20° C). Blood plasma osmolality increased with salinity at all temperatures. Standard metabolic rate (SMR) increased with salinity at 10 and 20° C. Maximum metabolic rate (MMR) and aerobic scope was lowest at salinity of 15 at 5° C, yet at 20° C, they were lowest at a salinity of 0. A cost of osmoregulation (SMR at a salinity of 0 and 15 compared with SMR at a salinity of 10) could only be detected at a salinity of 15 at 20° C, where it was 28%. The results show that P. fluviatilis have capacity to osmoregulate in hyper-osmotic environments. This contradicts previous studies and indicates intraspecific variability in osmoregulatory capabilities among P. fluviatilis populations or habitat origins. An apparent cost of osmoregulation (28%) at a salinity of 15 at 20° C indicates that the cost of osmoregulation in P. fluviatilis increases with temperature under hyperosmotic conditions and a power analysis showed that the cost of osmoregulation could be lower than 12·5% under other environmental conditions. The effect of salinity on MMR is possibly due to a reduction in gill permeability, initiated to reduce osmotic stress. An interaction between salinity and temperature on aerobic scope shows that high salinity habitats are energetically beneficial during warm periods (summer), whereas low salinity habitats are energetically beneficial during cold periods (winter). It is suggested, therefore, that the seasonal migrations of P. fluviatilis between brackish and fresh water is to select an environment that is optimal for metabolism and aerobic scope. © 2016 The Fisheries Society of the British Isles.

The transcriptomic responses of the eastern oyster, Crassostrea virginica, to environmental conditions.

PubMed

Chapman, Robert W; Mancia, Annalaura; Beal, Marion; Veloso, Artur; Rathburn, Charles; Blair, Anne; Holland, A F; Warr, G W; Didinato, Guy; Sokolova, Inna M; Wirth, Edward F; Duffy, Edward; Sanger, Denise

2011-04-01

Understanding the mechanisms by which organisms adapt to environmental conditions is a fundamental question for ecology and evolution. In this study, we evaluate changes in gene expression of a marine mollusc, the eastern oyster Crassostrea virginica, associated with the physico-chemical conditions and the levels of metals and other contaminants in their environment. The results indicate that transcript signatures can effectively disentangle the complex interactive gene expression responses to the environment and are also capable of disentangling the complex dynamic effects of environmental factors on gene expression. In this context, the mapping of environment to gene and gene to environment is reciprocal and mutually reinforcing. In general, the response of transcripts to the environment is driven by major factors known to affect oyster physiology such as temperature, pH, salinity, and dissolved oxygen, with pollutant levels playing a relatively small role, at least within the range of concentrations found in the studied oyster habitats. Further, the two environmental factors that dominate these effects (temperature and pH) interact in a dynamic and nonlinear fashion to impact gene expression. Transcriptomic data obtained in our study provide insights into the mechanisms of physiological responses to temperature and pH in oysters that are consistent with the known effects of these factors on physiological functions of ectotherms and indicate important linkages between transcriptomics and physiological outcomes. Should these linkages hold in further studies and in other organisms, they may provide a novel integrated approach for assessing the impacts of climate change, ocean acidification and anthropogenic contaminants on aquatic organisms via relatively inexpensive microarray platforms. © 2011 Blackwell Publishing Ltd.

Best-fit analysis for future coral reef survivors on Bonaire: A lifeline to the reefs' future in the region

NASA Astrophysics Data System (ADS)

Johnson, C. C.; Elswick, E. R.; Beeker, C. D.; Kauffman, E. G.; Budziak, A. T.; Wiegand, N.

2012-12-01

Given the decline of Caribbean corals and increases in environmental threats and human stressors to the reef ecosystem, it is imperative to document and establish a biological and environmental baseline inventory of coral recruits and environments in which corals live. Our project investigated the association of corals and water chemistry on the leeward side of Bonaire to test for and assess the hypothesis of ocean acidification affecting one of the healthiest reefs in the Caribbean. A dry island such as Bonaire, with no major river input into the leeward side of the island, provides an ideal location for such an analyses as it yields a relatively pure ocean chemistry signal. A multi-year investigation in Bonaire National Marine Park (BNMP) focused on corals growing on mooring buoy anchors created from cement blocks and cement filled, discarded petroleum barrels. We evaluate the persistence of corals on anchors placed in BNMP in the 1960's and 1970's, taking advantage of the maximum timeline for coral recruits. Recruits initiated in the zone occupied initially by Acropora cervicornis and A. palmata, and persisted through the decline of the once dominant acroporids. Thus, our study can be taken as a natural inoculation experiment under ambient field conditions. We collected 200ml water samples at 25 sites and analyzed samples on the Atomic Absorption Spectrometer Analyst 800 and Dionex IC25 Ion Chromatograph instruments to yield elemental data for water chemistry analyses. Depth pH, temperature, salinity and turbidity were recorded per site in rainy and dry seasons over the 5-year, Nov 2007 - April 2011 study. pH measurements were taken by colorimetric and indicator strips. Biologic data collection focused on coral species identifications per site on mooring anchors but sponges, hydrozoans and algae were also noted. Our research reveals no pH changes in these shallow (12.2m) waters over the duration of the study. pH colorimetric averages were 8.0-8.5 for both April/May and Nov/ Dec collections, and pH litmus 8.0 for all sites, all seasons. We further investigated the association of temperature, salinity and turbidity at site-specific locations and recorded differences in cation and anion water chemistry. Seasonal averages for T are 27.2oC for April/May and 28.1oC for Nov/Dec. Salinity seasonal averages are 37.4ppt for April/May and 36.7ppt for Nov/Dec; Mg/Ca (wt. ratio) exhibits seasonal variation; turbidity was 0 JTU for all sites, all collections. Our bioenvironmental database allows for ordination analysis of coral species with specific water chemistry conditions. Family Faviidae (Diploria labrynthiformis, D. strigosa), with origins in the mid-Jurassic geologic record, dominates the occurrences, followed by Family Poritidae (Porites asteroides) and Family Agariciidae, but great variability exists across sites. Nitrates, nitrites and phosphates, local human stressors, have variable effect on coral presence and persistence. The database provides the best-fit analysis for future survivors under climate change conditions and may be the lifeline to the reefs' future in this region.

Production of biomass by Spirulina at different groundwater type. Case of Ouargla-Southeast Algeria

NASA Astrophysics Data System (ADS)

Saggaï, Ali; Dadamoussa, Belkheir; Djaghoubi, Afaf; Bissati, Samia

2016-07-01

In this paper, Spirulina platensis was cultivated to estimate the biomass production with different groundwater type in Ouargla. Growth experiments were undertaken in flasks under shelter in outdoor condition. For this, the temperature, pH and salinity value was recorded between two days of growth. Biomass concentration in the culture media was calculated by measuring the DO625. The combination of the Mioplocen water with the nutriments gave the highest values of biomass concentration with avenge of 1.78 ±0.91g/l. All the three-type water supported the growth of Spirulina that appeared as good as a culture media.

Extremophiles for microbial-electrochemistry applications: A critical review.

PubMed

Shrestha, Namita; Chilkoor, Govinda; Vemuri, Bhuvan; Rathinam, Navanietha; Sani, Rajesh K; Gadhamshetty, Venkataramana

2018-05-01

Extremophiles, notably archaea and bacteria, offer a good platform for treating industrial waste streams that were previously perceived as hostile to the model organisms in microbial electrochemical systems (MESs). Here we present a critical overview of the fundamental and applied biology aspects of halophiles and thermophiles in MESs. The current study suggests that extremophiles enable the MES operations under a seemingly harsh conditions imposed by the physical (pressure, radiation, and temperature) and geochemical extremes (oxygen levels, pH, and salinity). We highlight a need to identify the underpinning mechanisms that define the exceptional electrocatalytic performance of extremophiles in MESs. Copyright © 2018 Elsevier Ltd. All rights reserved.

Seasonal microbial and environmental parameters at Crocker Reef, Florida Keys, 2014–2015

USGS Publications Warehouse

Kellogg, Christina A.; Yates, Kimberly K.; Lawler, Stephanie N.; Moore, Christopher S.; Smiley, Nathan A.

2015-11-04

Microbial measurements included enumeration of total bacteria, enumeration of virus-like particles, and plate counts of Vibrio spp. colony-forming units (CFU). These measurements were intended to give a sense of any seasonal changes in the total microbial load and to provide an indication of water quality. Additional environmental parameters measured included water temperature, salinity, dissolved oxygen, and pH. Four sites (table 1) were intensively sampled for periods of approximately 48 hours during summer (July 2014) and winter (January–February 2015), during which water samples were collected every 4 hours for analysis, except when prevented by weather conditions.

Extremozymes from Marine Actinobacteria.

PubMed

Suriya, J; Bharathiraja, S; Krishnan, M; Manivasagan, P; Kim, S-K

Marine microorganisms that have the possibility to survive in diverse conditions such as extreme temperature, pH, pressure, and salinity are known as extremophiles. They produce biocatalysts so named as extremozymes that are active and stable at extreme conditions. These enzymes have numerous industrial applications due to its distinct properties. Till now, only a fraction of microorganisms on Earth have been exploited for screening of extremozymes. Novel techniques used for the cultivation and production of extremophiles, as well as cloning and overexpression of their genes in various expression systems, will pave the way to use these enzymes for chemical, food, pharmaceutical, and other industrial applications. © 2016 Elsevier Inc. All rights reserved.

Species Profiles. Life Histories and Environmental Requirements of Coastal Fishes and Invertebrates (Pacific Northwest). Dungeness Crab.

DTIC Science & Technology

1986-08-01

variety of factors crab eggs has been linked to increased including depth, latitude, tempera- egg mortality because of mechanical ture, salinity and...time. crabs seem less dependent on epibenthic cover and can be found over more exposed substrates. Most crabs Temperature- Salinity Interactions remain...13 Salinity . .. ....... ........................................ 14 Temperature- Salinity Interactions. .. .... ....... ....... 14

Ocean acidification state in western Antarctic surface waters: drivers and interannual variability

NASA Astrophysics Data System (ADS)

Mattsdotter Björk, M.; Fransson, A.; Chierici, M.

2013-05-01

Each December during four years from 2006 to 2010, the surface water carbonate system was measured and investigated in the Amundsen Sea and Ross Sea, western Antarctica as part of the Oden Southern Ocean expeditions (OSO). The I/B Oden started in Punta Arenas in Chile and sailed southwest, passing through different regimes such as, the marginal/seasonal ice zone, fronts, coastal shelves, and polynyas. Discrete surface water was sampled underway for analysis of total alkalinity (AT), total dissolved inorganic carbon (CT) and pH. Two of these parameters were used together with sea-surface temperature (SST), and salinity to obtain a full description of the surface water carbonate system, including pH in situ and calcium carbonate saturation state of aragonite (ΩAr) and calcite (ΩCa). Multivariate analysis was used to investigate interannual variability and the major controls (sea-ice concentration, SST, salinity and chlorophyll a) on the variability in the carbonate system and Ω. This analysis showed that SST and chlorophyll a were the major drivers of the Ω variability in both the Amundsen and Ross seas. In 2007, the sea-ice edge was located further south and the area of the open polynya was relatively small compared to 2010. We found the lowest pH in situ (7.932) and Ω = 1 values in the sea-ice zone and in the coastal Amundsen Sea, nearby marine out flowing glaciers. In 2010, the sea-ice coverage was the largest and the areas of the open polynyas were the largest for the whole period. This year we found the lowest salinity and AT, coinciding with highest chl a. This implies that the highest ΩAr in 2010 was likely an effect of biological CO2 drawdown, which out-competed the dilution of carbonate ion concentration due to large melt water volumes. We predict and discuss future Ω values, using our data and reported rates of oceanic uptake of anthropogenic CO2, suggesting that the Amundsen Sea will become undersaturated with regard to aragonite about 20 yr sooner than predicted by models.

Seasonal variations of carbonate system parameters and nutrients at the shellfish-farming bays along the south coast of Korea

NASA Astrophysics Data System (ADS)

Shim, JeongHee; Shim, Jeong-Min; Lee, Yong-Hwa

2017-04-01

About 80 90% of the annual mass production of shellfish in Korea are cultured at the inner bays including Jinhae, Tongyeong and Geoje regions, along the south coast of Korea. To understand coastal carbon and nutrients cycles and those effects/feedbacks on shellfish farming, carbonate (DIC, TA and pH) and environmental parameters were observed at Jinhae, Tongyeong and Geoje Bays 4 times (in Feb., Aug. 2014, Apr. and Oct. 2015 and are considered representative of winter, summer, spring and fall respectively). Surface temperature in the bays showed clear seasonal variation with about 6 12°C and 24 29°C in Feb. and Aug. 2014, respectively and 14 18°C and 22 26°C in Apr. and in Oct. 2015, respectively. Surface pHNBS also ranged with about 8.20 8.53 and 7.28 8.95 in Feb. and Aug. 2014, and 8.04 8.40 and 7.91 8.32 in Apr. and in Oct. 2015. High pH with low salinity in summer resulted from input of land discharge in rainy seasons, however high pH at small bays in Apr. and Oct. 2015 resulted from massive primary production by phytoplankton bloom, supported by high chlorophyll a concentrations. Seasonal variations of DIC and phosphate in the surface and bottom waters correlated largely with salinity, higher in winter and lower in summer. Specifically in shellfish (specially, oyster and mussel) growing season, aragonite saturation state (Ωarag) in bottom water ranged about 0.2 2.9 (mean 2.1) and 2.2 5.0 (mean 3.2) in Feb. 2014 and Oct. 2015, respectively, suggesting low pH environments arose seasonally in coastal area due to some mechanisms. These results suggest that seasonal ocean acidification state might seriously affect shell growth, mass production and thus shellfish industry along the south coast of Korea.

The effects of mercury on developing larvae of Rhithropanopeus harrisii (Gould) . I. Interactions of temperature, salinity and mercuryon larval development

NASA Astrophysics Data System (ADS)

McKenney, C. L.; Costlow, J. D.

1982-02-01

Larvae of the estuarine xanthid crab Rhithropanopeus harrisii were reared inthe laboratory from hatch through completion of metamorphosis to postlarva in 64 combinations of temperature (20-35°C), salinity (10-40‰) and mercury (0-20 parts 10 -9 Hg 2+). Multiple linear regression analysis and response surface methodology were used to determine and visually display the individual and interactive effects of the various constant temperature-salinity-mercury combinations on both survival and developmental rates of developing larvae throughout total larval development and for zoeae and megalopae separately, to distinguish any differential sensitivity between the two larval forms. Survival capacity of larvae under a broad range of salinities and temperatures characteristic of temperate estuarine conditions was progressively reduced upon continual exposure to mercury ranging from 5 to 20 parts 10 -9 Hg 2+. Exposure concentrations as low as 5 parts 10 -9 Hg 2+ reduced the salinity and temperature plasciticity of the normally euryhalinic and eurythermal larvae. Larval survival from hatch to postlarva was affected by both mercury-salinity and mercury-temperature interactions with mercury toxicity increasing under suboptimal temperatures and salinities. Viability of early zoeal stages proved more sensitive to mercury exposure than the final megalopa stage. Exposure to mercury concentrations from 5-20 parts 10 -9, Hg 2+ prolonged complete developmental duration by 3 to 4 days with zoeal developmental rates retarded more than megalopal rates. Developmental rates of the megalopa were more reduced by mercury at higher salinities, and both zoeal and megalopal developmental rates were more retarded by mercury at lower temperatures. The reduced plasticity of larvae to estuarine conditions, and retarded developmental rates by low mercury concentrations may reduce recruitment into adult benthic populations and alter the distributional patterns of pelagic R. harrisii larvae.

Salinity and Temperature Tolerance Experiments on Selected Florida Bay Mollusks

USGS Publications Warehouse

Murray, James B.; Wingard, G. Lynn

2006-01-01

The ultimate goal of the Comprehensive Everglades Restoration Plan (CERP) is to restore and preserve the unique ecosystems of South Florida, including the estuaries. Understanding the effect of salinity and temperature changes, beyond typical oscillations, on the biota of South Florida's estuaries is a necessary component of achieving the goal of restoring the estuaries. The U.S. Geological Survey has been actively involved in researching the history of the South Florida Ecosystem, to provide targets, performance measures, and baseline data for restoration managers. These experiments addressed two aspects of ecosystem history research: 1) determining the utility of using molluscan shells as recorders of change in water chemistry parameters, primarily salinity, and 2) enhancing our in situ observations on modern assemblages by exceeding typically observed aquatic conditions. This set of experiments expanded our understanding of the effects of salinity, temperature and other water chemistry parameters on the reproduction, growth and overall survivability of key species of mollusks used in interpreting sediment core data. Observations on mollusks, plants and microbes made as part of these experiments have further refined our knowledge and understanding of the effects of ecosystem feedback and the role salinity and temperature play in ecosystem stability. The results have demonstrated the viability of several molluscan species as indicators of atypical salinity, and possibly temperature, modulations. For example Cerithium muscarum and Bulla striata demonstrated an ability to withstand a broad salinity and temperature range, with reproduction occurring in atypically high salinities and temperatures. These experiments also provided calibration data for the shell biogeochemistry of Chione cancellata and the possible use of this species as a water chemistry recorder. Observations made in the mesocosms, on a scale not normally observable in the field, have led to new questions about the influence of salinity on the localized ecosystem. The next phase of these experiments; to calibrate growth rate and reproductive viability in atypical salinities is currently underway.

Radioactivity and geochemistry of selected mineral-spring waters in the Western United States; basic data and multivariate statistical analysis

USGS Publications Warehouse

Felmlee, J.K.; Cadigan, R.A.

1982-01-01

Multivariate statistical analyses were performed on data from 156 mineral-spring sites in nine Western States to analyze relationships among the various parameters measured in the spring waters. Correlation analysis and R-mode factor analysis indicate that three major factors affect water composition in the spring systems studied: (1) duration of water circulation, (2) depth of water circulation, and (3) partial pressure of carbon dioxide. An examination of factor scores indicates that several types of hydrogeologic systems were sampled. Most of the samples are (1) older water from deeper circulating systems having relatively high salinity, high temperature, and low Eh or (2) younger water from shallower circulating systems having relatively low salinity, low temperature, and high Eh. The rest of the samples are from more complex systems. Any of the systems can have a relatively high or low content of dissolved carbonate species, resulting in a low or high pH, respectively. Uranium concentrations are commonly higher in waters of relatively low temperature and high Eh, and radium concentrations are commonly higher in waters having a relatively high carbonate content (low pH) and, secondarily, relatively high salinity. Water samples were collected and (or) measurements were taken at 156 of the 171 mineral-spring sites visited. Various samples were analyzed for radium, uranium, radon, helium, and radium-228 as well as major ions and numerous trace elements. On-site measurements for physical properties including temperature, specific conductance, pH, Eh, and dissolved oxygen were made. All constituents and properties show a wide range of values. Radium concentrations range from less than 0.01 to 300 picocuries per liter; they average 1.48 picocuries per liter and have an anomaly threshold value of 171 picocuries per liter for the samples studied. Uranium concentrations range from less than 0.01 to 120 micrograms per liter and average 0.26 micrograms per liter; they have an anomaly threshold value of 48.1 micrograms per liter. Radon content ranges from less than 10 to 110,000 picocuries per liter, averages 549 picocuries per liter and has an anomaly threshold of 20,400 picocuries per liter. Helium content ranges from -1,300 to +13,000 parts per billion relative to atmospheric helium; it averages +725 parts per billion and has an anomaly threshold of 10,000 parts per billion. Radium-228 concentrations range from less than 2.0 to 33 picocuries per liter; no anomaly threshold was determined owing to the small number of samples. All of the anomaly thresholds may be somewhat high because the sampling was biased toward springs likely to be radioactive. The statistical variance in radium and uranium concentrations unaccounted for by the identified factors testifies to the complexity of some hydrogeologic systems. Unidentified factors related to geologic setting and the presence of uranium-rich rocks in the systems also affect the observed concentrations of the radioactive elements in the water. The association of anomalous radioactivity in several springs with nearby known uranium occurrences indicates that other springs having anomalous radioactivity may also be associated with uranium occurrences as yet undiscovered.

Limacina retroversa's response to combined effects of ocean acidification and sea water freshening

NASA Astrophysics Data System (ADS)

Manno, C.; Morata, N.; Primicerio, R.

2012-11-01

Anthropogenic carbon dioxide emissions induce ocean acidification, thereby reducing carbonate ion concentration, which may affect the ability of calcifying organisms to build shells. Pteropods, the main planktonic producers of aragonite in the worlds' oceans, may be particularly vulnerable to changes in sea water chemistry. The negative effects are expected to be most severe at high-latitudes, where natural carbonate ion concentrations are low. In this study we investigated the combined effects of ocean acidification and freshening on Limacina retroversa, the dominant pteropod in sub polar areas. Living L. retroversa, collected in Northern Norwegian Sea, were exposed to four different pH values ranging from the pre-industrial level to the forecasted end of century ocean acidification scenario. Since over the past half-century the Norwegian Sea has experienced a progressive freshening with time, each pH level was combined with a salinity gradient in two factorial, randomized experiments investigating shell degradation, swimming behavior and survival. In addition, to investigate shell degradation without any physiologic influence, one perturbation experiments using only shells of dead pteropods was performed. Lower pH reduced shell mass whereas shell dissolution increased with pCO2. Interestingly, shells of dead organisms had a higher degree of dissolution than shells of living individuals. Mortality of Limacina retroversa was strongly affected only when both pH and salinity reduced simultaneously. The combined effects of lower salinity and lower pH also affected negatively the ability of pteropods to swim upwards. Results suggest that the energy cost of maintaining ion balance and avoiding sinking (in low salinity scenario) combined with the extra energy cost necessary to counteract shell dissolution (in high pCO2 scenario), exceed the available energy budget of this organism causing the pteropods to change swimming behavior and begin to collapse. Since L. retroversa play an important role in the transport of carbonates to the deep oceans these findings have significant implications for the mechanisms influencing the inorganic carbon cycle in the sub-polar area.

Clonal variation in response to salinity and flooding stress in four marsh macrophytes of the northern gulf of Mexico, USA

USGS Publications Warehouse

Howard, R.J.; Rafferty, P.S.

2006-01-01

Intraspecific variation in stress tolerance can be an important factor influencing plant population structure in coastal wetland habitats. We studied clones of four species of emergent marsh macrophytes native to the northern coast of the Gulf of Mexico in Louisiana, USA, to examine variation in response to salinity and flooding stress under controlled greenhouse conditions. Clones of Distichlis spicata, Phragmites australis, Schoenoplectus californicus, and Schoenoplectus robustus were collected across the coastal zone of Louisiana. After vegetative propagation through at least three generations to remove acclimation to field conditions, four to six clones of each species were selected for use in the experiment. Treatments consisted of three salinity levels and two water depths, and species were assigned to either a brackish marsh (P. australis, S. californicus) or salt marsh (D. spicata, S. robustus) group for treatment application. Treatment effects on plant growth (stem number, total height, and mean height, and aboveground and belowground biomass) were examined, and physicochemical characteristics within treatments (redox potential, and interstitial water pH, salinity, temperature, and nutrients) were monitored. Clonal variation in growth was indicated in all species, and was more pronounced in D. spicata and P. australis than in S. californicus and S. robustus. Distichlis spicata and P. australis clones were assigned to relative categories of low, intermediate, and high tolerance to the imposed stressors. Similar generalizations on clonal stress tolerance were not possible for the two Schoenoplectus species. Overall species response to imposed stressors was also identified through non-statistical comparisons. Phragmites australis was more tolerant than S. californicus of increased salinity. Distichlis spicata was more tolerant of increased salinity but less tolerant of increased water depth than was S. robustus. Our results suggest that information on species and clone variations in growth and tolerance to salinity and flooding stressors can be used to select a variety of plant materials suitable for wetland restoration projects.

Near-surface salinity and temperature structure observed with dual-sensor drifters in the subtropical South Pacific

NASA Astrophysics Data System (ADS)

Dong, Shenfu; Volkov, Denis; Goni, Gustavo; Lumpkin, Rick; Foltz, Gregory R.

2017-07-01

Three surface drifters equipped with temperature and salinity sensors at 0.2 and 5 m depths were deployed in April/May 2015 in the subtropical South Pacific with the objective of measuring near-surface salinity differences seen by satellite and in situ sensors and examining the causes of these differences. Measurements from these drifters indicate that water at a depth of 0.2 m is about 0.013 psu fresher than at 5 m and about 0.024°C warmer. Events with large temperature and salinity differences between the two depths are caused by anomalies in surface freshwater and heat fluxes, modulated by wind. While surface freshening and cooling occurs during rainfall events, surface salinification is generally observed under weak wind conditions (≤4 m/s). Further examination of the drifter measurements demonstrates that (i) the amount of surface freshening and strength of the vertical salinity gradient heavily depend on wind speed during rain events, (ii) salinity differences between 0.2 and 5 m are positively correlated with the corresponding temperature differences for cases with surface salinification, and (iii) temperature exhibits a diurnal cycle at both depths, whereas the diurnal cycle of salinity is observed only at 0.2 m when the wind speed is less than 6 m/s. The amplitudes of the diurnal cycles of temperature at both depths decrease with increasing wind speed. The mean diurnal cycle of surface salinity is dominated by events with winds less than 2 m/s.

Near-surface Salinity and Temperature structure Observed with Dual-Sensor Drifters in the Subtropical South Pacific

NASA Astrophysics Data System (ADS)

Dong, S.; Volkov, D.; Goni, G. J.; Lumpkin, R.; Foltz, G. R.

2017-12-01

Three surface drifters equipped with temperature and salinity sensors at 0.2 m and 5 m depths were deployed in April/May 2015 in the subtropical South Pacific with the objective of measuring near-surface salinity differences seen by satellite and in situ sensors and examining the causes of these differences. Measurements from these drifters indicate that water at a depth of 0.2 m is about 0.013 psu fresher than at 5 m and about 0.024°C warmer. Events with large temperature and salinity differences between the two depths are caused by anomalies in surface freshwater and heat fluxes, modulated by wind. While surface freshening and cooling occurs during rainfall events, surface salinification is generally observed under weak wind conditions (≤4 m/s). Further examination of the drifter measurements demonstrates that (i) the amount of surface freshening and strength of the vertical salinity gradient heavily depend on wind speed during rain events, (ii) salinity differences between 0.2 m and 5 m are positively correlated with the corresponding temperature differences for cases with surface salinification, and (iii) temperature exhibits a diurnal cycle at both depths, whereas the diurnal cycle of salinity is observed only at 0.2 m when the wind speed is less than 6 m/s. The amplitudes of the diurnal cycles of temperature at both depths decrease with increasing wind speed. The mean diurnal cycle of surface salinity is dominated by events with winds less than 2 m/s.

Reconstructing Past Ocean Salinity ((delta)18Owater)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Guilderson, T P; Pak, D K

2005-11-23

Temperature and salinity are two of the key properties of ocean water masses. The distribution of these two independent but related characteristics reflects the interplay of incoming solar radiation (insolation) and the uneven distribution of heat loss and gain by the ocean, with that of precipitation, evaporation, and the freezing and melting of ice. Temperature and salinity to a large extent, determine the density of a parcel of water. Small differences in temperature and salinity can increase or decrease the density of a water parcel, which can lead to convection. Once removed from the surface of the ocean where 'local'more » changes in temperature and salinity can occur, the water parcel retains its distinct relationship between (potential) temperature and salinity. We can take advantage of this 'conservative' behavior where changes only occur as a result of mixing processes, to track the movement of water in the deep ocean (Figure 1). The distribution of density in the ocean is directly related to horizontal pressure gradients and thus (geostrophic) ocean currents. During the Quaternary when we have had systematic growth and decay of large land based ice sheets, salinity has had to change. A quick scaling argument following that of Broecker and Peng [1982] is: the modern ocean has a mean salinity of 34.7 psu and is on average 3500m deep. During glacial maxima sea level was on the order of {approx}120m lower than present. Simply scaling the loss of freshwater (3-4%) requires an average increase in salinity a similar percentage or to {approx}35.9psu. Because much of the deep ocean is of similar temperature, small changes in salinity have a large impact on density, yielding a potentially different distribution of water masses and control of the density driven (thermohaline) ocean circulation. It is partly for this reason that reconstructions of past salinity are of interest to paleoceanographers.« less

Survival and development of horseshoe crab (Limulus polyphemus) embryos and larvae in hypersaline conditions.

PubMed

Ehlinger, Gretchen S; Tankersley, Richard A

2004-04-01

The horseshoe crab Limulus polyphemus spawns in the mid- to upper intertidal zone where females deposit eggs in nests below the sediment surface. Although adult crabs generally inhabit subtidal regions of estuaries with salinities from 5 to 34 ppt, developing embryos and larvae within nests are often exposed to more extreme conditions of salinity and temperature during summer spawning periods. To test whether these conditions have a negative impact on early development and survival, we determined development time, survival, and molt cycle duration for L. polyphemus embryos and larvae raised at 20 combinations of salinity (range: 30-60 ppt) and temperature (range: 25-40 degrees C). Additionally, the effect of hyperosmotic and hypoosmotic shock on the osmolarity of the perivitelline fluid of embryos was determined at salinities between 5 and 90 ppt. The embryos completed their development and molted at salinities below 60 ppt, yet failed to develop at temperatures of 35 degrees C or higher. Larval survival was high at salinities of 10-70 ppt but declined significantly at more extreme salinities (i.e., 5, 80, and 90 ppt). Perivitelline fluid remained nearly isoosmotic over the range of salinities tested. Results indicate that temperature and salinity influence the rate of crab development, but only the extremes of these conditions have an effect on survival.

Revisiting carbonate chemistry controls on planktic foraminifera Mg / Ca: implications for sea surface temperature and hydrology shifts over the Paleocene-Eocene Thermal Maximum and Eocene-Oligocene transition

NASA Astrophysics Data System (ADS)

Evans, David; Wade, Bridget S.; Henehan, Michael; Erez, Jonathan; Müller, Wolfgang

2016-04-01

Much of our knowledge of past ocean temperatures comes from the foraminifera Mg / Ca palaeothermometer. Several nonthermal controls on foraminifera Mg incorporation have been identified, of which vital effects, salinity, and secular variation in seawater Mg / Ca are the most commonly considered. Ocean carbonate chemistry is also known to influence Mg / Ca, yet this is rarely examined as a source of uncertainty, either because (1) precise pH and [CO32-] reconstructions are sparse or (2) it is not clear from existing culture studies how a correction should be applied. We present new culture data of the relationship between carbonate chemistry and Mg / Ca for the surface-dwelling planktic species Globigerinoides ruber and compare our results to data compiled from existing studies. We find a coherent relationship between Mg / Ca and the carbonate system and argue that pH rather than [CO32-] is likely to be the dominant control. Applying these new calibrations to data sets for the Paleocene-Eocene Thermal Maximum (PETM) and Eocene-Oligocene transition (EOT) enables us to produce a more accurate picture of surface hydrology change for the former and a reassessment of the amount of subtropical precursor cooling for the latter. We show that pH-adjusted Mg / Ca and δ18O data sets for the PETM are within error of no salinity change and that the amount of precursor cooling over the EOT has been previously underestimated by ˜ 2 °C based on Mg / Ca. Finally, we present new laser-ablation data of EOT-age Turborotalia ampliapertura from St. Stephens Quarry (Alabama), for which a solution inductively coupled plasma mass spectrometry (ICPMS) Mg / Ca record is available (Wade et al., 2012). We show that the two data sets are in excellent agreement, demonstrating that fossil solution and laser-ablation data may be directly comparable. Together with an advancing understanding of the effect of Mg / Casw, the coherent picture of the relationship between Mg / Ca and pH that we outline here represents a step towards producing accurate and quantitative palaeotemperatures using this proxy.

Examination of factors dominating the sediment-water diffusion flux of DDT-related compounds measured by passive sampling in an urbanized estuarine bay.

PubMed

Feng, Yan; Wu, Chen-Chou; Bao, Lian-Jun; Shi, Lei; Song, Lin; Zeng, Eddy Y

2016-12-01

The fate of hydrophobic organic compounds in aquatic environment are largely determined by their exchange at sediment-water interface, which is highly dynamic and subject to rapidly evolving environmental conditions. In turn, environmental conditions may be governed by both physicochemical parameters and anthropogenic events. To examine the importance of various impact factors, passive sampling devices were deployed at the seafloor of Hailing Bay, an urbanized estuarine bay in Guangdong Province of South China to measure the sediment-water diffusion fluxes of several metabolites of dichlorodiphenyltrichloroethane (DDT), p,p'-DDE, p,p'-DDD and o,p'-DDD. The physicochemical properties of water (temperature, pH, salinity and dissolved oxygen) and surface sediment (sediment organic matter, physical composition, pH, water content, colony forming unit and catalase activity) were also measured. The results showed that the diffusion fluxes of o,p'-DDD, p,p'-DDD and p,p'-DDE at sites A1 and A2 near a fishing boat maintenance facility ranged from 0.42 to 4.73 ng m -2 d -1 (from sediment to overlying water), whereas those at offshore sites varied between -0.03 and -3.02 ng m -2 d -1 (from overlying water to sediment), implicating A1 and A2 as the sources of the target compounds. The distribution patterns of the diffusion fluxes of the target compounds were different from those of water and sediment parameters (water temperature, salinity, sediment texture, pH, colony forming unit and catalase activity) at six sampling sites. This finding suggested that none of these parameters were critical in dictating the sediment-water diffusion fluxes. Besides, decreases in the contents of kerogen and black carbon by 6.7% and 11% would enhance the diffusion fluxes of the target compounds by 11-14% and 12-23%, respectively, at site A1, indicating that kerogen and black carbon were the key factors in mediating the sediment-water diffusion fluxes of DDT-related compounds in field environments. Copyright © 2016 Elsevier Ltd. All rights reserved.

Molecular cloning and sequence analysis of two carbonic anhydrase in the swimming crab Portunus trituberculatus and its expression in response to salinity and pH stress.

PubMed

Pan, Luqing; Hu, Dongxu; Liu, Maoqi; Hu, Yanyan; Liu, Shengnan

2016-01-15

Carbonic anhydrase (CA) is involved in ion transport, acid-base balance and pH regulation by catalyzing the interconversion of CO2 and HCO3(-). In this study, full-length cDNA sequences of two CA isoforms were identified from Portunus trituberculatus. One was Portunus trituberculatus cytoplasmic carbonic anydrase (PtCAc) and the other one was Portunus trituberculatus glycosyl-phosphatidylinositol-linked carbonic anhydrase (PtCAg). The sequence of PtCAc was formed by an ORF of 816 bp, encoding a protein of 30.18 kDa. The PtCAg was constituted by an ORF of 927 bp, encoding a protein of 34.09 kDa. The deduced amino acid sequences of the two CA isoforms were compared to other crustacean' CA sequences. Both of them reflected high conservation of the residues and domains essential to the function of the two enzymes. The tissue expression analysis of PtCAc and PtCAg were detected in gill, muscle, hepatopancreas, hemocytes and gonad. PtCAc and PtCAg gene expressions were studied under salinity and pH challenge. The results showed that when salinity decreased (30 to 20 ppt), the mRNA expression of PtCAc increased significantly at 24 and 48 h, and the highest value appeared at 24h. The mRNA expression of PtCAg had the same situation with PtCAc. However, when salinity increased (30 to 35 ppt), only the mRNA expression of PtCAc increased significantly at 48 h. When pH changed, only the mRNA expression of PtCAc increased significantly at 12h, which was under low pH situation. The mRNA expression of PtCAg increased significantly at 12-48 h, and there was no significant difference of the expression between the pH challenged group and the control group in other experimental time. The results provided the base of understanding CA' function and the underlying mechanism in response to environmental changes in crustaceans. Copyright © 2015 Elsevier B.V. All rights reserved.

Submesoscale-selective compensation of fronts in a salinity-stratified ocean.

PubMed

Spiro Jaeger, Gualtiero; Mahadevan, Amala

2018-02-01

Salinity, rather than temperature, is the leading influence on density in some regions of the world's upper oceans. In the Bay of Bengal, heavy monsoonal rains and runoff generate strong salinity gradients that define density fronts and stratification in the upper ~50 m. Ship-based observations made in winter reveal that fronts exist over a wide range of length scales, but at O(1)-km scales, horizontal salinity gradients are compensated by temperature to alleviate about half the cross-front density gradient. Using a process study ocean model, we show that scale-selective compensation occurs because of surface cooling. Submesoscale instabilities cause density fronts to slump, enhancing stratification along-front. Specifically for salinity fronts, the surface mixed layer (SML) shoals on the less saline side, correlating sea surface salinity (SSS) with SML depth at O(1)-km scales. When losing heat to the atmosphere, the shallower and less saline SML experiences a larger drop in temperature compared to the adjacent deeper SML on the salty side of the front, thus correlating sea surface temperature (SST) with SSS at the submesoscale. This compensation of submesoscale fronts can diminish their strength and thwart the forward cascade of energy to smaller scales. During winter, salinity fronts that are dynamically submesoscale experience larger temperature drops, appearing in satellite-derived SST as cold filaments. In freshwater-influenced regions, cold filaments can mark surface-trapped layers insulated from deeper nutrient-rich waters, unlike in other regions, where they indicate upwelling of nutrient-rich water and enhanced surface biological productivity.

Plankton community and the relationship with the environment in saline lakes of Onon-Torey plain, Northeastern Mongolia.

PubMed

Afonina, Ekaterina Yu; Tashlykova, Natalya A

2018-02-01

The plankton community of sixteen saline lakes located on Onon-Torey plain (Northeastern Mongolia) during the filling phase and the raising of the water level was investigated in July 2011. Thirty-five taxa of phytoplankton and thirty-one species of zooplankton were found. For phytoplankton, blue-green algae ( Merismopedia elegans , Anabaenopsis elenkinii , Arthrospora fusiformis , Spirulina major , Lyngbya sp., Oscillatoria sp.) and green algae ( Monoraphidium minutum , Tetrastrum komarekii , Ankyra ocellata , Oocystis sp.) were dominant. For zooplankton, Filinia longiseta, Brachionus plicatilis , B. variabilis , Hexarthra mira (Rotifera), Daphnia magna , Moina brachiata , M. mongolica (Cladocera), Arctodiaptomus bacillifer , Mixodiaptomus incrassatus , Metadiaptomus asiaticus (Copepoda) dominated. Mineralization, active hydrogen ratio, dissolved oxygen and water temperature were the main factors influencing the diversity, structure and distribution of plankton organisms in the steppe lakes during low water level. The RDA analysis for phytoplankton and zooplankton from different lakes was carried out for selected two groups which included lakes and a subset related species. The first group is of oligohaline and mesohaline lakes in which mostly green algae, rotifers and copepods inhabit. The second group is of mesohaline and polyhaline lakes with mainly blue-green algae , some crustaceans and rotifers inhabiting. High abundance and biomass of Spirulina major , Oscillatoria sp. and Brachionus variabilis were observed in lakes with high mineralization, pH and temperature.

Geochemistry of Dissolved Trace Metals in the Waters of Bahia Magdalena, Baja California Sur, Pacific Coast, Mexico

NASA Astrophysics Data System (ADS)

Suresh Babu, S.

2016-12-01

Forty two samples were acquired from the surface and bottom water profiles along 5 transects spread over Bahia Magdalena lagoon, Baja California Sur to assess the behavior of trace metals in a high influenced upwelling region on the Pacific coast. To elaborate the fate of metals, also the physico-chemical parameters (pH, temperature, salinity, conductivity, dissolved oxygen). Determination of the concentrations of trace metals (Fe, Mn, Cr, Cu, Co, Pb, Ni, Zn, Cd As, Hg) were measured using Atomic absorption spectrometry. The results demonstrated high values of As, Ni and Co which is attributed to the local geology and phosphate deposits. Low values of Fe and Mn are attested to the oxic conditions of the lagoon which are responsible for the oxidation of Fe and Mn. The region witnesses raised temperatures (28.92ºC) and salinities of 35.2 PSU for its arid climatic conditions and high rates of evaporation. In general, the region presented minor quantities of dissolved trace metals due to dispersion and high intense interaction with the open sea. The results were also compared with other studies to understand the enrichment pattern in this side of the pacific coast which experiences various geothermal activities and upwelling phenomenon.

Carbon isotope variations in a solar pond microbial mat: Role of environmental gradients as steering variables

NASA Astrophysics Data System (ADS)

Schidlowski, Manfred; Gorzawski, Hendrik; Dor, Inka

1994-05-01

A biogeochemical traverse is presented for a juvenile benthic mat covering the depth profile of an artificially stratified and eutrophicated hypersaline heliothermal pond with known gradients of temperature, salinity, pH, and light transmission. It can be shown that visual mat development depends primarily on temperature and salinity as main environmental steering variables whose increase with depth goes along with the attenuation and final disappearance of a visible microbial film in the pond's hypolimnic compartment. Recorded biogeochemical parameters (C org content, cell numbers, chlorophyll-a content) evidently reflect, as either biomass- or productivity-related index functions, the visually perceptible growth gradient of the microbial ecosystem along the pond slope. The observed coincidence of maxima in these index functions with maxima in δ13Corg clearly identifies high rates of primary productivity as the agent ultimately responsible for the generation of isotopically heavy ( 13C-enriched) biomass in these and related environments. Extreme demands placed on the local feeder pool of dissolved inorganic carbon by high rates of primary productivity entertained by the mat-forming microbenthos obviously give rise to severe CO 2 limitation, enforcing the operation of a diffusion-(supply-)limited assimilatory pathway with an isotopically indiscriminate metabolization of the available CO 2 resources.

Evaluation of a Prototype pCO2 Optical Sensor

NASA Astrophysics Data System (ADS)

Sanborn-Marsh, C.; Sutton, A.; Sabine, C. L.; Lawrence-Salvas, N.; Dietrich, C.

2016-12-01

Anthropogenic greenhouse gas emissions continue to rise, driving climate change and altering the ocean carbonate systems. Carbonate chemistry can be characterized by any two of the four parameters: pH, total alkalinity, dissolved inorganic carbon, and partial pressure of dissolved carbon dioxide gas (pCO2). To fully monitor these dynamic systems, researchers must deploy a more temporally and spatially comprehensive sensor network. Logistical challenges, such as the energy consumption, size, lifetime, depth range, and cost of pCO2 sensors have limited the network's reach so far. NOAA's Pacific Marine Environmental Laboratory has conducted assessment tests of a pCO2 optical sensor (optode), recently developed by Atamanchuk et al (2014). We hope to deploy this optode in the summer of 2017 on high-resolution moored profiler, along with temperature, salinity, and oxygen sensors. While most pCO2 optodes have energy consumptions of 3-10 W, this 36mm-diameter by 86mm-long instrument consumes a mere 7-80 mW. Initial testing showed that its accuracy varied within an absolute range of 2-75 μatm, depending on environmental conditions, including temperature, salinity, response time, and initial calibration. Further research independently examining the effects of each variable on the accuracy of the data will also be presented.

Europa's Compositional Evolution and Ocean Salinity

NASA Astrophysics Data System (ADS)

Vance, S.; Glein, C.; Bouquet, A.; Cammarano, F.; McKinnon, W. B.

2017-12-01

Europa's ocean depth and composition have likely evolved through time, in step with the temperature of its mantle, and in concert with the loss of water and hydrogen to space and accretion of water and other chemical species from comets, dust, and Io's volcanism. A key aspect to understanding the consequences of these processes is combining internal structure models with detailed calculations of ocean composition, which to date has not been done. This owes in part to the unavailability of suitable thermodynamic databases for aqueous chemistry above 0.5 GPa. Recent advances in high pressure aqueous chemistry and water-rock interactions allow us to compute the equilibrium ionic conditions and pH everywhere in Europa's interior. In this work, we develop radial structure and composition models for Europa that include self-consistent thermodynamics of all materials, developed using the PlanetProfile software. We will describe the potential hydration states and porosity of the rocky interior, and the partitioning of primordial sulfur between this layer, an underlying metallic core, and the ocean above. We will use these results to compute the ocean's salinity by extraction from the upper part of the rocky layer. In this context, we will also consider the fluxes of reductants from Europa's interior due to high-temperature hydrothermalism, serpentinization, and endogenic radiolysis.

ELECTROPHORESIS EXPERIMENTS WITH THE VIRUS AND PROTECTIVE BODIES OF YELLOW FEVER

PubMed Central

Frobisher, Martin

1931-01-01

1. When suspended in slightly alkaline (pH 7.4 to 7.8) saline dilutions of clear, hemoglobin-free normal monkey serum, the virus of yellow fever from infected monkeys and from infected, but blood-free, mosquitoes, usually acts as if it were possessed of a positive electrical charge. 2. The virus tends to assume a negative charge in fluids having a slightly acid reaction. 3. The isoelectric point of the virus seems to be in the neighborhood of pH 7.0, possibly ranging from pH 7.3 to pH 6.9. 4. Exposure to fluid having a reaction of pH 5.0 for 3 hours appeared to inactivate the virus. 5. In experiments in which the suspending fluid was prepared with normal serum diluted with distilled water and containing a good quantity of partly hemolyzed erythrocytes, the virus tended to migrate to the anode. 6. The protective bodies in yellow fever immune serum appear to carry a negative charge in slightly alkaline saline dilutions of serum. PMID:19869954

Combined Effect of Ocean Acidification and Seawater Freshening: Response of Pteropod Swimming Behavior

NASA Astrophysics Data System (ADS)

Manno, C.; Morata, N.; Primicerio, R.

2012-12-01

Increasing anthropogenic carbon dioxide emissions induce ocean acidification. Pteropods, the main planktonic producers of aragonite in the worlds' oceans, may be particularly vulnerable to changes in sea water chemistry. The negative effects are expected to be most severe at high-latitudes, where natural carbonate ion concentrations are low. In this study we investigated the combined effects of ocean acidification and freshening on Limacina retroversa, the dominant pteropod in sub polar areas. Living Limacina retroversa, collected in Northern Norwegian Sea, were exposed to four different pH values ranging from the pre-industrial level to the forecasted end of century ocean acidification scenario. Since over the past half-century the Norwegian Sea has experienced a progressive freshening with time, each pH level was combined with a salinity gradient. Survival, shell degradation and swimming behavior were investigated. Mortality was strongly affected only when both pH and salinity reduced simultaneously. The combined effects of lower salinity and lower pH also affected negatively the ability of pteropods to swim where they decreasing the locomotory speed upwards and increasing the wing beats. Results suggest that, the extra energy cost due to maintaining of body fluids and to avoid sinking (in low salinity scenario) combined with the extra energy cost necessary to counteract the dissolution (in high pCO2 scenario), exceeds the available energy budget of this organism and then pteropods change in swimming behavior and begin to collapse. Since Limacina retroversa play an important role in the transport of carbonates to the deep oceans these findings have significant implications for the mechanisms influencing the inorganic carbon cycle in the sub-polar area.

Growth of the Facultative Anaerobes from Antarctica, Alaska, and Patagonia at Low Temperatures

NASA Technical Reports Server (NTRS)

Pikuta, Elena V.; Hoover, Richard B.

2004-01-01

Psychotolerance, as an adaptation for surviving in extreme environments, is widespread among mesophilic microorganisms. Physico-chemical factors such as pressure, red-ox potential, pH and salinity could significantly alter the features of ecosystems by providing liquid water at subzero temperatures. Furthermore, organisms can respond to temperature changes by several known mechanisms, including changing the conformation capacities of constitutional proteins or by the synthesis of mucopolysaccharides around the cell wall and membrane. Such protective mechanisms make it possible for cells to not only passively survive low temperatures in a state of anabiosis, but also to be capable of actively metabolizing substrates and reproducing normally. The physiological and biochemical characteristics of the species, as well as genetics, could be remarkably changed due to adaptation and surviving in extreme environments. The cold shock genes of some of the studied strains of psychotolerant facultative anaerobes were reported previously. In this paper we present experimental data for psychotolerant, non spore-forming, facultative anaerobes isolated from geographically different cold regions of our planet. We show the growth response on changing from anaerobic conditions to aerobic with cultivation at low temperatures.

Influence of salinity and temperature on acute toxicity of cadmium to Mysidopsis bahia molenock

DOE Office of Scientific and Technical Information (OSTI.GOV)

Voyer, R.A.; Modica, G.

1990-01-01

Acute toxicity tests were conducted to compare estimates of toxicity, as modified by salinity and temperature, based on response surface techniques with those derived using conventional test methods, and to compare effect of a single episodic exposure to cadmium as a function of salinity with that of continuous exposure. Regression analysis indicated that mortality following continuous 96-hr exposure is related to linear and quadratic effects of salinity and cadmium at 20 C, and to the linear and quadratic effects of cadmium only at 25C. LC50s decreased with increases in temperature and decreases in salinity. Based on the regression model developed,more » 96-hr LC50s ranged from 15.5 to 28.0 micro Cd/L at 10 and 30% salinities, respectively, at 25C; and from 47 to 85 microgram Cd/L at these salinities at 20C.« less

Temperature-salinity structure of the AMOC in high-resolution ocean simulations and in CMIP5 models

NASA Astrophysics Data System (ADS)

Wang, F.; Xu, X.; Chassignet, E.

2017-12-01

On average, the CMIP5 models represent the AMOC structure, water properties, Heat transport and Freshwater transport reasonably well. For temperature, CMIP5 models exhibit a colder northward upper limb and a warmer southward lower limb. the temperature contrast induces weaker heat transport than observation. For salinity, CMIP5 models exhibit saltier southward lower limb, thus contributes to weaker column freshwater transport. Models have large spread, among them, AMOC strength contributes to Heat transport but not freshwater transport. AMOC structure (the overturning depth) contributes to transport-weighted temperature not transport-weighted salinity in southward lower limb. The salinity contrast in upper and lower limb contributes to freshwater transport, but temperature contrast do not contribute to heat transport.

Interactive effects of pH, EC and nitrogen on yields and nutrient absorption of rice (Oryza sativa L.)

USDA-ARS?s Scientific Manuscript database

Soil salinity and sodicity can not only directly restrain crop growth by osmotic and specific ion stresses, it also may reduce grain yield indirectly by impacting plant absorption of essential nutrients. Ensuring adequate nitrogen is an important management aspect of rice production in saline-sodic ...

Environmental factors limiting fertilisation and larval success in corals

NASA Astrophysics Data System (ADS)

Woods, Rachael M.; Baird, Andrew H.; Mizerek, Toni L.; Madin, Joshua S.

2016-12-01

Events in the early life history of reef-building corals, including fertilisation and larval survival, are susceptible to changes in the chemical and physical properties of sea water. Quantifying how changes in water quality affect these events is therefore important for understanding and predicting population establishment in novel and changing environments. A review of the literature identified that levels of salinity, temperature, pH, suspended sediment, nutrients and heavy metals affect coral early life-history stages to various degrees. In this study, we combined published experimental data to determine the relative importance of sea water properties for coral fertilisation success and larval survivorship. Of the water properties manipulated in experiments, fertilisation success was most sensitive to suspended sediment, copper, salinity, phosphate and ammonium. Larval survivorship was sensitive to copper, lead and salinity. A combined model was developed that estimated the joint probability of both fertilisation and larval survivorship in sea water with different chemical and physical properties. We demonstrated the combined model using water samples from Sydney and Lizard Island in Australia to estimate the likelihood of larvae surviving through both stages of development to settlement competency. Our combined model could be used to recommend targets for water quality in coastal waterways as well as to predict the potential for species to expand their geographical ranges in response to climate change.

[Research on mercury methylation by Geobacter sulfurreducens and its influencing factors].

PubMed

Zou, Yan; Si, You-Bin; Yan, Xue; Chen, Yan

2012-09-01

Mercury methylation by Geobacter sulfurreducens and the effects of environmental factors were studied under laboratory conditions. The results showed that G. sulfurreducens could grow well in the presence of low concentrations of mercuric chloride, but its growth was inhibited to a certain extent, mainly expressed in the prolonged lag phase. G. sulfurreducens could transform inorganic mercury into methylmercury, and this process was affected by many environmental factors. The efficiency of mercury methylation reached 38% under anaerobic conditions with 1 mg x L(-1) HgCl2 and 0.9% salinity at 35 degrees C, pH 6.0. Increasing the initial HgCl2 concentration or salinity in an appropriate manner improved mercury methylation, but the concentration of methylmercury reduced when the concentrations of HgCl2 and salinity were too high. The efficiency of mercury methylation increased with the increasing temperature in range of 4-35 degrees C. Weakly acidic environment was more beneficial to mercury methylation than acidic, neutral or alkaline conditions. In addition, the efficiency of mercury methylation was also affected by humic acid and cysteine. Humic acid inhibited mercury methyaltion, whereas cysteine could improve the efficiency of mercury methylation. This study provided a direct evidence for mercury methylation mediated by iron-reducing bacteria in the natural aquatic ecosystem.

Acidophilic Halophilic Microorganisms in Fluid Inclusions in Halite from Lake Magic, Western Australia

PubMed Central

Conner, Amber J.

2013-01-01

Abstract Lake Magic is one of the most extreme of hundreds of ephemeral acid-saline lakes in southern Western Australia. It has pH as low as 1.7, salinity as high as 32% total dissolved solids, temperatures ranging from 0°C to 50°C, and an unusually complex aqueous composition. Optical petrography, UV-vis petrography, and laser Raman spectrometry were used to detect microorganisms and organic compounds within primary fluid inclusions in modern bedded halite from Lake Magic. Rare prokaryotes appear as 1–3 μm, bright cocci that fluoresce green with UV-vis illumination. Dimpled, 5–7 μm yellow spherules that fluoresce blue with UV-vis illumination are interpreted as Dunaliella algae. Yellow-orange beta-carotene crystals, globules, and coatings are characterized by orange-red fluorescence and three distinct Raman peaks. Because acid saline lakes are good Mars analogues, the documentation of prokaryotes, eukaryotes, and organic compounds preserved in the halite here has implications for the search for life on Mars. Missions to Mars should incorporate such in situ optical and chemical examination of martian evaporites for possible microorganisms and/or organic compounds in fluid inclusions. Key Words: Acid—Extremophiles—Western Australia—Fluid inclusions—Lake Magic—Dunaliella. Astrobiology 13, 850–860. PMID:23971647

Distribution of the Euryhaline Squid Lolliguncula brevis in Chesapeake Bay: Effects of Selected Abiotic Factors

DTIC Science & Technology

2002-01-31

salinity , water temperature, dissolved oxy- gen and water clarity. Since temporal variation in the Chesapeake Bay ecosystem is high, the effects of year...temperature (p ɘ.001; ψ = 2.42) had significant impacts on squid catch probability, although the effects were con- founded by a water temperature × salinity ...commonly encountered in such waters during VIMS Trawl Surveys The synergistic and independent effects of salinity , water temperature and dissolved oxygen

The Effects of Temperature and Salinity on Mg Incorporation in Planktonic Foraminifera Globigerinoides ruber (white): Results from a Global Sediment Trap Mg/Ca Database

NASA Astrophysics Data System (ADS)

Gray, W. R.; Weldeab, S.; Lea, D. W.

2015-12-01

Mg/Ca in Globigerinoides ruber is arguably the most important proxy for sea surface temperature (SST) in tropical and sub tropical regions, and as such guides our understanding of past climatic change in these regions. However, the sensitivity of Mg/Ca to salinity is debated; while analysis of foraminifera grown in cultures generally indicates a sensitivity of 3 - 6% per salinity unit, core-top studies have suggested a much higher sensitivity of between 15 - 27% per salinity unit, bringing the utility of Mg/Ca as a SST proxy into dispute. Sediment traps circumvent the issues of dissolution and post-depositional calcite precipitation that hamper core-top calibration studies, whilst allowing the analysis of foraminifera that have calcified under natural conditions within a well constrained period of time. We collated previously published sediment trap/plankton tow G. ruber (white) Mg/Ca data, and generated new Mg/Ca data from a sediment trap located in the highly-saline tropical North Atlantic, close to West Africa. Calcification temperature and salinity were calculated for the time interval represented by each trap/tow sample using World Ocean Atlas 2013 data. The resulting dataset comprises >240 Mg/Ca measurements (in the size fraction 150 - 350 µm), that span a temperature range of 18 - 28 °C and 33.6 - 36.7 PSU. Multiple regression of the dataset reveals a temperature sensitivity of 7 ± 0.4% per °C (p < 2.2*10-16) and a salinity sensitivity of 4 ± 1% per salinity unit (p = 2*10-5). Application of this calibration has significant implications for both the magnitude and timing of glacial-interglacial temperature changes when variations in salinity are accounted for.

[Joint effects of water temperature and salinity on the expression of gill Hsp70 gene in Pinctada martensii (Dunker)].

PubMed

Wang, Ya-Nan; Wang, Hui; Zhu, Xiao-Wen; Luo, Ming-Ming; Liu, Zhi-Gang; Du, Xiao-Dong

2012-12-01

By using central composite experimental design and response surface method, the joint effects of water temperature (16-40 degrees C) and salinity (10-50) on the expression of gill Hsp70 gene in Pinctada martensii (Dunker) were studied under laboratory conditions. The results showed that the linear and quadratic effects of temperature on the expression of gill Hsp70 gene were significant, the linear effect of salinity was not significant, while the quadratic effect of salinity was significant. The interactive effect of temperature and salinity was not significant, and the effect of temperature was greater than that of salinity. The model equation of the gill Hsp70 gene expression was established, with the R2, Adj. R2, and Pred. R2 as high as 98.7%, 97.4%, and 89.2%, respectively, suggesting that the overarching predictive capability of the model was very satisfactory, and could be practicably applied for prediction. Through the optimization of the model, the expression of the gill Hsp70 gene reached its minimum (0.5276) when the temperature was 26.78 degrees C and the salinity was 29.33, with the desirability value being 98%. These experimental results could offer theoretical reference for the high expression of gill Hsp70 gene in P. martensii, the maintenance of cell internal environment stability, and the enhancement of P. martensii stress resistance.

The combined influence of sub-optimal temperature and salinity on the in vitro viability of Perkinsus marinus, a protistan parasite of the eastern oyster Crassostrea virginica

USGS Publications Warehouse

La Peyre, M.K.; Casas, S.M.; Gayle, W.; La Peyre, Jerome F.

2010-01-01

Perkinsus marinus is a major cause of mortality in eastern oysters along the Gulf of Mexico and Atlantic coasts. It is also well documented that temperature and salinity are the primary environmental factors affecting P. marinus viability and proliferation. However, little is known about the effects of combined sub-optimal temperatures and salinities on P. marinus viability. This in vitro study examined those effects by acclimating P. marinus at three salinities (7, 15, 25. ppt) to 10 ??C to represent the lowest temperatures generally reached in the Gulf of Mexico, and to 2 ??C to represent the lowest temperatures reached along the mid-Atlantic coasts and by measuring changes in cell viability and density on days 1, 30, 60 and 90 following acclimation. Cell viability and density were also measured in 7. ppt cultures acclimated to each temperature and then transferred to 3.5. ppt. The largest decreases in cell viability occurred only with combined low temperature and salinity, indicating that there is clearly a synergistic effect. The largest decreases in cell viability occurred only with both low temperature and salinity after 30. days (3.5. ppt, 2 ??C: 0% viability), 60. days (3.5. ppt, 10 ??C: 0% viability) and 90. days (7. ppt, 2 ??C: 0.6 ?? 0.7%; 7. ppt, 10 ??C: 0.2 ?? 0.2%). ?? 2010 .

Discoloration of titanium alloy in acidic saline solutions with peroxide.

PubMed

Takemoto, Shinji; Hattori, Masayuki; Yoshinari, Masao; Kawada, Eiji; Oda, Yutaka

2013-01-01

The objective of this study was to compare corrosion behavior in several titanium alloys with immersion in acidulated saline solutions containing hydrogen peroxide. Seven types of titanium alloy were immersed in saline solutions with varying levels of pH and hydrogen peroxide content, and resulting differences in color and release of metallic elements determined in each alloy. Some alloys were characterized using Auger electron spectroscopy. Ti-55Ni alloy showed a high level of dissolution and difference in color. With immersion in solution containing hydrogen peroxide at pH 4, the other alloys showed a marked difference in color but a low level of dissolution. The formation of a thick oxide film was observed in commercially pure titanium showing discoloration. The results suggest that discoloration in titanium alloys immersed in hydrogen peroxide-containing acidulated solutions is caused by an increase in the thickness of this oxide film, whereas discoloration of Ti-55Ni is caused by corrosion.

Influence of nitrate, sulfate and operational parameters on the bioreduction of perchlorate using an up-flow packed bed reactor at high salinity.

PubMed

Chung, J; Shin, S; Oh, J

2010-05-01

In this study we have investigated whether electron acceptors, such as nitrate or sulphate ions, competitively inhibit the reduction of perchlorate in brine in continuous up-flow packed bed bioreactors. The effect of pH and hydraulic retention time (HRT) on the reduction of perchlorate at high salinity has also been examined. Reduction of perchlorate was found to be only moderately influenced by nitrate (under 163 mg N L-'), implying that there was no significant microbial competition for electron acceptors. As a result of microbial diversity, there were few differences between microbial communities fed with a variety of media, suggesting that most nitrate-reducing bacteria are able to reduce perchlorate at high salinity. Reduction of perchlorate was almost complete at relatively high sulfate levels (1000 mg L(-1)), neutral pH (6-8) and relatively long HRTs (> 10 h).

Analysis of Factors Influencing Soil Salinity, Acidity, and Arsenic Concentration in a Polder in Southwest Bangladesh

NASA Astrophysics Data System (ADS)

Ayers, J. C.; Patton, B.; Fry, D. C.; Goodbred, S. L., Jr.

2017-12-01

Soil samples were collected on Polder 32 in the coastal zone of SW Bangladesh in wet (October) and dry (May) seasons from 2013-2017 and analyzed to characterize the problems of soil salinization and arsenic contamination and identify their causes. Soils are entisols formed from recently deposited, predominantly silt-sized sediments with low carbon concentrations typical of the local mangrove forests. Soluble (DI extract) arsenic concentrations were below the Government of Bangladesh limit of 50 ppb for drinking water. Soil acidity and extract arsenic concentrations exhibit spatial variation but no consistent trends. In October soil extract As is higher and S and pH are lower than in May. These observations suggest that wet season rainwater oxidizes pyrite, reducing soil S and releasing H+, causing pH to decrease. Released iron is oxidized to form Hydrous Ferric Oxyhydroxides (HFOs), which sorb As and increase extractable As in wet season soils. Changes in pH are small due to pH buffering by soil carbonates. Soil and rice paddy water salinities are consistently higher in May than October, reaching levels in May that reduce rice yields. Rice grown in paddies should be unaffected by salt concentrations in the wet season, while arsenic concentrations in soil may be high enough to cause unsafe As levels in produced rice.

Hypothalamic GABAergic influences on treadmill exercise responses in rats.

PubMed

Overton, J M; Redding, M W; Yancey, S L; Stremel, R W

1994-01-01

Microinjection of GABAergic antagonists in the posterior hypothalamus (PH) produces exercise-like adjustments in cardiovascular function. To test the hypothesis that a hypothalamic GABAergic mechanism within the PH modulates the cardiovascular adjustments to dynamic exercise in conscious animals, Sprague-Dawley rats (n = 10) were instrumented with bilateral guide cannula directed at the pH, an arterial cannula, and Doppler flow probes on the iliac and mesenteric arteries. Saline (100 nl) or the GABAA receptor agonist muscimol (125 ng.100 nl-1) was bilaterally injected into the PH during treadmill exercise (20 m.min-1). Microinjection of saline had no effect on mean arterial pressure (MAP), heart rate (HR), mesenteric vascular resistance (MR), or iliac vascular resistance (IR) during exercise. Microinjection of muscimol during exercise produced no significant changes in MAP (mean change +/- SE; +0 +/- 1 mmHg), HR (+17 +/- 12 b.min-1), or MR (+7 +/- 13%). However, microinjection of muscimol produced a significant increase in IR during exercise (16 +/- 6%). In addition, muscimol significantly decreased treadmill run time (saline = 19.6 +/- 0.4 min; muscimol = 17.8 +/- 0.6 min) and produced behavioral effects (including mild sedation) that were most evident after exercise. The results of these experiments suggest that while the posterior hypothalamic GABAergic system may modulate iliac blood flow during exercise in rats, this system does not modulate HR and MR responses to dynamic exercise.

Quantification of surface charge density and its effect on boundary slip.

PubMed

Jing, Dalei; Bhushan, Bharat

2013-06-11

Reduction of fluid drag is important in the micro-/nanofluidic systems. Surface charge and boundary slip can affect the fluid drag, and surface charge is also believed to affect boundary slip. The quantification of surface charge and boundary slip at a solid-liquid interface has been widely studied, but there is a lack of understanding of the effect of surface charge on boundary slip. In this paper, the surface charge density of borosilicate glass and octadecyltrichlorosilane (OTS) surfaces immersed in saline solutions with two ionic concentrations and deionized (DI) water with different pH values and electric field values is quantified by fitting experimental atomic force microscopy (AFM) electrostatic force data using a theoretical model relating the surface charge density and electrostatic force. Results show that pH and electric field can affect the surface charge density of glass and OTS surfaces immersed in saline solutions and DI water. The mechanisms of the effect of pH and electric field on the surface charge density are discussed. The slip length of the OTS surface immersed in saline solutions with two ionic concentrations and DI water with different pH values and electric field values is measured, and their effects on the slip length are analyzed from the point of surface charge. Results show that a larger absolute value of surface charge density leads to a smaller slip length for the OTS surface.

Computing the carbonate chemistry of the coral calcifying medium and its response to ocean acidification.

PubMed

Raybaud, Virginie; Tambutté, Sylvie; Ferrier-Pagès, Christine; Reynaud, Stéphanie; Venn, Alexander A; Tambutté, Éric; Nival, Paul; Allemand, Denis

2017-07-07

Critical to determining vulnerability or resilience of reef corals to Ocean Acidification (OA) is a clearer understanding of the extent to which corals can control carbonate chemistry in their Extracellular Calcifying Medium (ECM) where the CaCO 3 skeleton is produced. Here, we employ a mathematical framework to calculate ECM aragonite saturation state (Ω arag.(ECM) ) and carbonate system ion concentration using measurements of calcification rate, seawater characteristics (temperature, salinity and pH) and ECM pH (pH (ECM) ). Our calculations of ECM carbonate chemistry at current-day seawater pH, indicate that Ω arag.(ECM) ranges from ∼10 to 38 (mean 20.41), i.e. about 5 to 6-fold higher than seawater. Accordingly, Dissolved Inorganic Carbon (DIC) and Total Alkalinity (TA) were calculated to be around 3 times higher in the ECM than in seawater. We also assessed the effects of acidification on ECM chemical properties of the coral Stylophora pistillata. At reduced seawater pH our calculations indicate that Ω arag.(ECM) remains almost constant. DIC (ECM) and TA (ECM) gradually increase as seawater pH declines, reaching values about 5 to 6-fold higher than in seawater, respectively for DIC and TA. We propose that these ECM characteristics buffer the effect of acidification and explain why certain corals continue to produce CaCO 3 even when seawater chemistry is less favourable. Copyright © 2017 Elsevier Ltd. All rights reserved.

Estuarine turbidity, flushing, salinity, and circulation

NASA Technical Reports Server (NTRS)

Pritchard, D. W.

1972-01-01

The effects of estuarine turbidity, flushing, salinity, and circulation on the ecology of the Chesapeake Bay are discussed. The sources of fresh water, the variations in salinity, and the circulation patterns created by temperature and salinity changes are analyzed. The application of remote sensors for long term observation of water temperatures is described. The sources of sediment and the biological effects resulting from increased sediments and siltation are identified.

Marine bivalve geochemistry and shell ultrastructure from modern low pH environments

NASA Astrophysics Data System (ADS)

Hahn, S.; Rodolfo-Metalpa, R.; Griesshaber, E.; Schmahl, W. W.; Buhl, D.; Hall-Spencer, J. M.; Baggini, C.; Fehr, K. T.; Immenhauser, A.

2011-10-01

Bivalve shells can provide excellent archives of past environmental change but have not been used to interpret ocean acidification events. We investigated carbon, oxygen and trace element records from different shell layers in the mussels Mytilus galloprovincialis (from the Mediterranean) and M. edulis (from the Wadden Sea) combined with detailed investigations of the shell ultrastructure. Mussels from the harbour of Ischia (Mediterranean, Italy) were transplanted and grown in water with mean pHT 7.3 and mean pHT 8.1 near CO2 vents on the east coast of the island of Ischia. The shells of transplanted mussels were compared with M. edulis collected at pH ~8.2 from Sylt (German Wadden Sea). Most prominently, the shells recorded the shock of transplantation, both in their shell ultrastructure, textural and geochemical record. Shell calcite, precipitated subsequently under acidified seawater responded to the pH gradient by an in part disturbed ultrastructure. Geochemical data from all test sites show a strong metabolic effect that exceeds the influence of the low-pH environment. These field experiments showed that care is needed when interpreting potential ocean acidification signals because various parameters affect shell chemistry and ultrastructure. Besides metabolic processes, seawater pH, factors such as salinity, water temperature, food availability and population density all affect the biogenic carbonate shell archive.

Evaluation of formation water chemistry and scale prediction: Bakken Shale

DOE PAGES

Thyne, Geoffrey; Brady, Patrick

2016-10-24

Determination of in situ formation water chemistry is an essential component of reservoir management. This study details the use of thermodynamic computer models to calculate reservoir pH and restore produced water analyses for prediction of scale formation. Bakken produced water samples were restored to formation conditions and calculations of scale formation performed. In situ pH is controlled by feldspar-clay equilibria. Calcite scale is readily formed due to changes in pH during pressure drop from in situ to surface conditions. The formation of anhydrite and halite scale, which has been observed, was predicted only for the most saline samples. Finally, inmore » addition, the formation of anhydrite and/or halite may be related to the localized conditions of increased salinity as water is partitioned into the gas phase during production.« less

Soil respiration in typical plant communities in the wetland surrounding the high-salinity Ebinur Lake

NASA Astrophysics Data System (ADS)

Li, Yanhong; Zhao, Mingliang; Li, Fadong

2018-03-01

Soil respiration in wetlands surrounding lakes is a vital component of the soil carbon cycle in arid regions. However, information remains limited on the soil respiration around highly saline lakes during the plant growing season. Here, we aimed to evaluate diurnal and seasonal variation in soil respiration to elucidate the controlling factors in the wetland of Ebinur Lake, Xinjiang Uygur Autonomous Region, western China. We used a soil carbon flux automatic analyzer (LI-840A) to measure soil respiration rates during the growing season (April to November) in two fields covered by reeds and tamarisk and one field with no vegetation (bare soil) from 2015 to 2016. The results showed a single peak in the diurnal pattern of soil respiration from 11:00 to 17:00 for plots covered in reeds, tamarisk, and bare soil, with minimum values being detected from 03:00 to 07:00. During the growing season, the soil respiration of reeds and tamarisk peaked during the thriving period (4.16 and 3.75 mmol•m-2•s-1, respectively), while that of bare soil peaked during the intermediate growth period (0.74 mmol•m-2•s-1). The soil respiration in all three plots was lowest during the wintering period (0.08, 0.09, and-0.87 mmol•m-2•s-1, respectively). Air temperature and relative humidity significantly influenced soil respiration. A significant linear relationship was detected between soil respiration and soil temperature for reeds, tamarisk, and bare soil. The average Q10 of reeds and tamarisk were larger than that of bare soil. However, soil moisture content was not the main factor controlling soil respiration. Soil respiration was negatively correlated with soil pH and soil salinity in all three plot types. In contrast, soil respiration was positively correlated with organic carbon. Overall, CO2 emissions and greenhouse gases had a relatively weak effect on the wetlands surrounding the highly saline Ebinur Lake.

Impact of long-term salinity exposure in anaerobic membrane bioreactors treating phenolic wastewater: Performance robustness and endured microbial community.

PubMed

Muñoz Sierra, Julian D; Oosterkamp, Margreet J; Wang, Wei; Spanjers, Henri; van Lier, Jules B

2018-05-07

Industrial wastewaters are becoming increasingly associated with extreme conditions such as the presence of refractory compounds and high salinity that adversely affect biomass retention or reduce biological activity. Hence, this study evaluated the impact of long-term salinity increase to 20 gNa + .L -1 on the bioconversion performance and microbial community composition in anaerobic membrane bioreactors treating phenolic wastewater. Phenol removal efficiency of up to 99.9% was achieved at 14 gNa + .L -1 . Phenol conversion rates of 5.1 mgPh.gVSS -1 .d -1 , 4.7 mgPh.gVSS -1 .d -1 , and 11.7 mgPh.gVSS -1 .d -1 were obtained at 16 gNa + .L -1 ,18 gNa + .L -1 and 20 gNa + .L -1 , respectively. The AnMBR's performance was not affected by short-term step-wise salinity fluctuations of 2 gNa + .L -1 in the last phase of the experiment. It was also demonstrated in batch tests that the COD removal and methane production rate were higher at a K + :Na + ratio of 0.05, indicating the importance of potassium to maintain the methanogenic activity. The salinity increase adversely affected the transmembrane pressure likely due to a particle size decrease from 185 μm at 14 gNa + .L -1 to 16 μm at 20 gNa + .L -1 . Microbial community was dominated by bacteria belonging to the Clostridium genus and archaea by Methanobacterium and Methanosaeta genus. Syntrophic phenol degraders, such as Pelotomaculum genus were found to be increased when the maximum phenol conversion rate was attained at 20 gNa + .L -1 . Overall, the observed robustness of the AnMBR performance indicated an endured microbial community to salinity changes in the range of the sodium concentrations applied. Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.

Few Like it Hot: Coral Reef Reponses to Elevated Temperatures and CO2

NASA Astrophysics Data System (ADS)

Eakin, C. M.; Gledhill, D. K.; Heron, S. F.; Skirving, W.; Christensen, T.; Morgan, J.; Liu, G.; Strong, A. E.

2007-12-01

Coral reefs live within a fairly narrow envelope of environmental conditions constrained by water temperatures, light, salinity, nutrients, bathymetry and the aragonite saturation state of seawater. As documented in numerous studies, the world's coral reefs are "in crisis" as a result of human impacts on their environment. While local stresses currently dominate, coral reefs are increasingly confronted with global-scale changes due to rising greenhouse gas concentrations. These changes are rapidly modifying the environmental envelope of coral reefs through both increased thermal stress and ocean acidification. In the former case, there is a well-documented relationship between thermal stress and the response of corals that include coral bleaching, disease, and mortality. Clear tolerance thresholds exist beyond which high temperature and accumulated thermal stress have deleterious effects. However, the synergistic effects of increasing temperature and ocean acidification are not yet fully understood. At this time, there is mounting concern that decreasing pH and aragonite saturation state will cause net reef accretion to cease or become negative. The threshold at which this could occur is likely to be reached much sooner than the pH drop necessary to induce carbonate dissolution. Both the thermal and chemical limits that control coral survival and reef growth will likely be passed before 2100 assuming even conservative projections reported in the 4th Assessment Report of the Intergovernmental Panel on Climate Change. This talk will discuss these thresholds and their ramifications for ecosystems and resource management.

The search for life on Europa: limiting environmental factors, potential habitats, and Earth analogues.

PubMed

Marion, Giles M; Fritsen, Christian H; Eicken, Hajo; Payne, Meredith C

2003-01-01

The putative ocean of Europa has focused considerable attention on the potential habitats for life on Europa. By generally clement Earth standards, these Europan habitats are likely to be extreme environments. The objectives of this paper were to examine: (1) the limits for biological activity on Earth with respect to temperature, salinity, acidity, desiccation, radiation, pressure, and time; (2) potential habitats for life on Europa; and (3) Earth analogues and their limitations for Europa. Based on empirical evidence, the limits for biological activity on Earth are: (1) the temperature range is from 253 to 394 K; (2) the salinity range is a(H2O) = 0.6-1.0; (3) the desiccation range is from 60% to 100% relative humidity; (4) the acidity range is from pH 0 to 13; (5) microbes such as Deinococcus are roughly 4,000 times more resistant to ionizing radiation than humans; (6) the range for hydrostatic pressure is from 0 to 1,100 bars; and (7) the maximum time for organisms to survive in the dormant state may be as long as 250 million years. The potential habitats for life on Europa are the ice layer, the brine ocean, and the seafloor environment. The dual stresses of lethal radiation and low temperatures on or near the icy surface of Europa preclude the possibility of biological activity anywhere near the surface. Only at the base of the ice layer could one expect to find the suitable temperatures and liquid water that are necessary for life. An ice layer turnover time of 10 million years is probably rapid enough for preserving in the surface ice layers dormant life forms originating from the ocean. Model simulations demonstrate that hypothetical oceans could exist on Europa that are too cold for biological activity (T < 253 K). These simulations also demonstrate that salinities are high, which would restrict life to extreme halophiles. An acidic ocean (if present) could also potentially limit life. Pressure, per se, is unlikely to directly limit life on Europa. But indirectly, pressure plays an important role in controlling the chemical environments for life. Deep ocean basins such as the Mariana Trench are good analogues for the cold, high-pressure ocean of Europa. Many of the best terrestrial analogues for potential Europan habitats are in the Arctic and Antarctica. The six factors likely to be most important in defining the environments for life on Europa and the focus for future work are liquid water, energy, nutrients, low temperatures, salinity, and high pressures.

Bolus oral or continuous intestinal amino acids reduce hypothermia during anesthesia in rats.

PubMed

Imoto, Akinobu; Yokoyama, Takeshi; Suwa, Kunio; Yamasaki, Fumiyasu; Yatabe, Tomoaki; Yokoyama, Reiko; Yamashita, Koichi; Selldén, Eva

2010-01-01

We hypothesized that, with oral or intestinal administration of amino acids (AA), we may reduce hypothermia during general anesthesia as effectively as with intravenous AA. We, therefore, examined the effect of bolus oral and continuous intestinal AA in preventing hypothermia in rats. Male Wistar rats were anesthetized with sevoflurane for induction and with propofol for maintenance. In the first experiment, 30 min before anesthesia, rats received one bolus 42 mL/kg of AA solution (100 g/L) or saline orally. Then for the next 3 h during anesthesia, they received 14 mL/kg/h of AA and/or saline intravenously. They were in 4 groups: I-A/A, both AA; I-A/S, oral AA and intravenous saline; I-S/A, oral saline and intravenous AA; I-S/S, both saline. In the second experiment, rats received 14 mL/kg/h duodenal AA and/or saline for 2 h. They were in 3 groups: II-A/S, duodenal AA and intravenous saline; II-S/A, duodenal saline and intravenous AA; II-S/S, both saline. Core body temperature was measured rectally. After the second experiment, serum electrolytes were examined. In both experiments, rectal temperature decreased in all groups during anesthesia. However, the decrease in rectal temperature was significantly less in groups receiving AA than in groups receiving only saline. In the second experiment, although there was no significant difference in the decrease in body temperature between II-A/S and II-S/A, Na(+) concentration was significantly lower in II-S/A. In conclusion, AA, administered orally or intestinally, tended to keep the body temperature stable during anesthesia without disturbing electrolyte balance. These results suggest that oral or enteral AA may be useful for prevention of hypothermia in patients.

Responses of trophic structure and zooplankton community to salinity and temperature in Tibetan lakes: Implication for the effect of climate warming.

PubMed

Lin, Qiuqi; Xu, Lei; Hou, Juzhi; Liu, Zhengwen; Jeppesen, Erik; Han, Bo-Ping

2017-11-01

Warming has pronounced effects on lake ecosystems, either directly by increased temperatures or indirectly by a change in salinity. We investigated the current status of zooplankton communities and trophic structure in 45 Tibetan lakes along a 2300 m altitude and a 76 g/l salinity gradient. Freshwater to hyposaline lakes mainly had three trophic levels: phytoplankton, small zooplankton and fish/Gammarus, while mesosaline to hypersaline lakes only had two: phytoplankton and large zooplankton. Zooplankton species richness declined significantly with salinity, but did not relate with temperature. Furthermore, the decline in species richness with salinity in lakes with two trophic levels was much less abrupt than in lakes with three trophic levels. The structural variation of the zooplankton community depended on the length of the food chain, and was significantly explained by salinity as the critical environmental variable. The zooplankton community shifted from dominance of copepods and small cladoceran species in the lakes with low salinity and three trophic levels to large saline filter-feeding phyllopod species in those lakes with high salinity and two trophic levels. The zooplankton to phytoplankton biomass ratio was positively related with temperature in two-trophic-level systems and vice versa in three-trophic-level systems. As the Tibetan Plateau is warming about three times faster than the global average, our results imply that warming could have a considerable impact on the structure and function of Tibetan lake ecosystems, either via indirect effects of salinization/desalinization on species richness, composition and trophic structure or through direct effects of water temperature on trophic interactions. Copyright © 2017 Elsevier Ltd. All rights reserved.

Effects of Physiochemical Factors on Prokaryotic Biodiversity in Malaysian Circumneutral Hot Springs.

PubMed

Chan, Chia S; Chan, Kok-Gan; Ee, Robson; Hong, Kar-Wai; Urbieta, María S; Donati, Edgardo R; Shamsir, Mohd S; Goh, Kian M

2017-01-01

Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity.

Effects of Physiochemical Factors on Prokaryotic Biodiversity in Malaysian Circumneutral Hot Springs

PubMed Central

Chan, Chia S.; Chan, Kok-Gan; Ee, Robson; Hong, Kar-Wai; Urbieta, María S.; Donati, Edgardo R.; Shamsir, Mohd S.; Goh, Kian M.

2017-01-01

Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3–V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334–26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity. PMID:28729863

Cell-specific effects of luminal acid, bicarbonate, cAMP, and carbachol on transporter trafficking in the intestine

PubMed Central

Jakab, Robert L.; Collaco, Anne M.

2012-01-01

Changes in intestinal luminal pH affect mucosal ion transport. The aim of this study was to compare how luminal pH and specific second messengers modulate the membrane traffic of four major ion transporters (CFTR, NHE3, NKCC1, and NBCe1) in rat small intestine. Ligated duodenal, jejunal, and ileal segments were infused with acidic or alkaline saline, 8-Br-cAMP, or the calcium agonist carbachol in vivo for 20 min. Compared with untreated intestine, lumen pH was reduced after cAMP or carbachol and increased following HCO3−-saline. Following HCl-saline, lumen pH was restored to control pH levels. All four secretory stimuli resulted in brush-border membrane (BBM) recruitment of CFTR in crypts and villi. In villus enterocytes, CFTR recruitment was coincident with internalization of BBM NHE3 and basolateral membrane recruitment of the bicarbonate transporter NBCe1. Both cAMP and carbachol recruited NKCC1 to the basolateral membrane of enterocytes, while luminal acid or HCO3− retained NKCC1 in intracellular vesicles. Luminal acid resulted in robust recruitment of CFTR and NBCe1 to their respective enterocyte membrane domains in the upper third of the villi; luminal HCO3− induced similar membrane changes lower in the villi. These findings indicate that each stimulus promotes a specific transporter trafficking response along the crypt-villus axis. This is the first demonstration that physiologically relevant secretory stimuli exert their actions in villus enterocytes by membrane recruitment of CFTR and NBCe1 in tandem with NHE3 internalization. PMID:22936272

Cell-specific effects of luminal acid, bicarbonate, cAMP, and carbachol on transporter trafficking in the intestine.

PubMed

Jakab, Robert L; Collaco, Anne M; Ameen, Nadia A

2012-10-15

Changes in intestinal luminal pH affect mucosal ion transport. The aim of this study was to compare how luminal pH and specific second messengers modulate the membrane traffic of four major ion transporters (CFTR, NHE3, NKCC1, and NBCe1) in rat small intestine. Ligated duodenal, jejunal, and ileal segments were infused with acidic or alkaline saline, 8-Br-cAMP, or the calcium agonist carbachol in vivo for 20 min. Compared with untreated intestine, lumen pH was reduced after cAMP or carbachol and increased following HCO(3)(-)-saline. Following HCl-saline, lumen pH was restored to control pH levels. All four secretory stimuli resulted in brush-border membrane (BBM) recruitment of CFTR in crypts and villi. In villus enterocytes, CFTR recruitment was coincident with internalization of BBM NHE3 and basolateral membrane recruitment of the bicarbonate transporter NBCe1. Both cAMP and carbachol recruited NKCC1 to the basolateral membrane of enterocytes, while luminal acid or HCO(3)(-) retained NKCC1 in intracellular vesicles. Luminal acid resulted in robust recruitment of CFTR and NBCe1 to their respective enterocyte membrane domains in the upper third of the villi; luminal HCO(3)(-) induced similar membrane changes lower in the villi. These findings indicate that each stimulus promotes a specific transporter trafficking response along the crypt-villus axis. This is the first demonstration that physiologically relevant secretory stimuli exert their actions in villus enterocytes by membrane recruitment of CFTR and NBCe1 in tandem with NHE3 internalization.

Effect of the phosphogypsum amendment of saline and agricultural soils on growth, productivity and antioxidant enzyme activities of tomato (Solanum lycopersicum L.).

PubMed

Smaoui-Jardak, Mariem; Kriaa, Walid; Maalej, Mohamed; Zouari, Mohamed; Kamoun, Lotfi; Trabelsi, Wassim; Ben Abdallah, Ferjani; Elloumi, Nada

2017-10-01

The objective of this study was to investigate the effects of phosphogypsum (PG) amendment on the physiochemical proprieties of saline and agricultural soils along with the growth, productivity and antioxidant enzyme activities of tomato plants ( Solanum lycopersicum L.) grown on the amended soils under controlled conditions. Obtained results showed that the amendment of saline soil (H) by PG induced a decrease in pH as well as in electrical conductivity. However, for the non saline soil (MC), there was a decrease in pH associated with an increase in electrical conductivity. For both soils, PG amendment led to an increase in Calcium (Ca) and sodium (Na), and a decrease in potassium (K) in plant tissues. Cadmium (Cd), Zinc (Zn) and Chromium (Cr) contents in different parts of plants increased in proportion with PG concentration in the soils. Apart from Cd, all the analyzed metals in tomato fruit were found to be below the recommended maximum allowable concentration (MAC). Our results showed that PG application, at doses not exceeding 20%, seems to be beneficial for growth, photosynthetic activity and productivity of tomato plants as well as in decreasing salinity of saline soils. In these conditions, the use of PG could be a promising project for the rehabilitation of marginalized and saline ecosystems with either ornamental or non-fruit species. For both soils, a significant accumulation of MDA in shoots was detected, reflecting cell membrane damage especially when the PG amendment reached 20%. Beyond 20 and 40% PG, tomato plants developed an enzymatic antioxidant defense system in response to salinity and heavy metal stress. However, at 80% PG, enzymes activities were significantly inhibited.

Submesoscale-selective compensation of fronts in a salinity-stratified ocean

PubMed Central

Spiro Jaeger, Gualtiero; Mahadevan, Amala

2018-01-01

Salinity, rather than temperature, is the leading influence on density in some regions of the world’s upper oceans. In the Bay of Bengal, heavy monsoonal rains and runoff generate strong salinity gradients that define density fronts and stratification in the upper ~50 m. Ship-based observations made in winter reveal that fronts exist over a wide range of length scales, but at O(1)-km scales, horizontal salinity gradients are compensated by temperature to alleviate about half the cross-front density gradient. Using a process study ocean model, we show that scale-selective compensation occurs because of surface cooling. Submesoscale instabilities cause density fronts to slump, enhancing stratification along-front. Specifically for salinity fronts, the surface mixed layer (SML) shoals on the less saline side, correlating sea surface salinity (SSS) with SML depth at O(1)-km scales. When losing heat to the atmosphere, the shallower and less saline SML experiences a larger drop in temperature compared to the adjacent deeper SML on the salty side of the front, thus correlating sea surface temperature (SST) with SSS at the submesoscale. This compensation of submesoscale fronts can diminish their strength and thwart the forward cascade of energy to smaller scales. During winter, salinity fronts that are dynamically submesoscale experience larger temperature drops, appearing in satellite-derived SST as cold filaments. In freshwater-influenced regions, cold filaments can mark surface-trapped layers insulated from deeper nutrient-rich waters, unlike in other regions, where they indicate upwelling of nutrient-rich water and enhanced surface biological productivity. PMID:29507874

The function profile of compressed-air and ultrasonic nebulizers.

PubMed

Wu, Hsin-Lin; Lin, Yung-Zen; Wu, Wei-Fong; Huang, Fu-Yuan

2003-01-01

In order to study the detailed function of two kinds of nebulizers commonly used in clinical asthma treatment, compressed-air and ultrasonic, this study was conducted. At the beginning, various flow rates were adjusted, paired with different volumes of solutions in the container. The changes of temperature, pH, and osmolality during the course of nebulization were examined. Normal saline, terbutaline, and fenoterol solutions were used as the nebulized solutions. The study was performed in an environment in ambient temperature around 20 degrees C and relative humidity around 70%. The results showed a minimal 6 L/min flow rate was required to nebulize the solution when using the compressed-air nebulizer. The dead volume was about 0.8 ml for compressed-air and 8.5 ml for the ultrasonic nebulizer. When using the compressed-air nebulizer, the temperature, both in the solution and at the mouthpiece site, dropped gradually. On the contrary, the temperatures at both sites increased a little bit when using the ultrasonic nebulizer. The pH values of pure terbutaline and fenoterol nebulized solutions were acidic (3.58 and 3.00 respectively). The osmolality of terbutaline and fenoterol nebulized solutions were isotonic. The osmolality increased gradually during the course of nebulization, to a greater extent in the compressed-air nebulizer. In conclusion, both types of nebulizers have their special features. The ultrasonic nebulizer displays less extent in change of temperature and osmolality during nebulization and is expected to be a better device in treating asthmatic patients in terms of lesser effect on cooling and changing the osmolality of airway mucosa.

The Removal of Terrestrial Dissolved Organic Matter in Coastal Regions by Photo-Flocculation Process

NASA Astrophysics Data System (ADS)

Abdulla, H. A.; Mopper, K.

2015-12-01

The fate of terrestrial dissolved organic matter (tDOM) as it moves to open ocean was the focus of many studies for the last three decades, most of these studies were focused on three major removal processes: 1) Photochemical mineralization of tDOM (conversion to inorganic forms); 2) Microbial oxidation; and 3) Mixing-induced flocculation. Based on recent estimations, the combination of theses removal processes accounts for ~20-35% of the loss of tDOM in estuaries and coastal regions; which is far from closing the gap between the riverine fluxes of tDOM and the amount of tDOM detected in the open ocean. In a preliminary experiment to determine if photo-flocculation indeed occurs at pH values and ionic strengths found in estuaries. A 0.1-μm filtered riverine was diluted 1:1 with artificial seawater and MilliQ water to yield final salinities ranging from 0 - 15; the pH of the saline samples was ranged from 6-8. Photo-flocculation was observed for all salinities, with particles organic carbon (POC) values ranged from 3.2 to 8.5% of the original DOC. Interestingly, the composition of the Photo-flocculated particles in the saline samples was markedly different from the zero salinity samples as shown in their FT-IR spectra. The photo-flocculated particles that formed in the saline samples appear to be rich in carbohydrate and amide functionalities (protein-like), while containing insignificant deprotonated carboxylate. While the flocs that formed in freshwater (salinity zero) are richer in deprotonated carboxyl groups, and relatively depleted in carbohydrate functionality.

The Predictability of Near-Coastal Currents Using a Baroclinic Unstructured Grid Model

DTIC Science & Technology

2011-12-28

clinic simulations. ADCIRC solves the time-dependent scalar transport equation for salinity and temperature. Through the equation of state...described by McDougall ct al. (2003), ADCIRC uses the temperature, salinity , and pressure in determining the density field. In order to avoid spurious...model. 2.3 Initialization and boundary forcing Temperature, salinity , elevation, and velocity fields from a regional ocean model are needed both to

Effect of salinity on the upper lethal temperature tolerance of early-juvenile red drum.

PubMed

McDonald, Dusty; Bumguardner, Britt; Cason, Paul

2015-10-01

Previous work investigating the temperature tolerance of juvenile red drum ranging 18-50mm TL found evidence for positive size dependence (smaller fish less tolerant to higher temperatures) suggesting smaller size classes (<18mm TL) potentially may succumb to extreme summer water temperatures. Here, we explored the upper lethal temperature tolerance (ULT) in smaller-sized red drum which ranged from 10 to 20mm TL across multiple salinities to further understand the thermal limitations of this propagated game fish. In order to investigate the combined effect of temperature and salinity on ULT, temperature trials were conducted under three levels of salinity which commonly occur along the coast of Texas (25, 35, and 45ppt). The rate of temperature increase (+0.25°C/h) was designed to mimic a natural temperature increase of a summer day in Texas. We determined that the lethal temperature at 50% (LT50) did not differ between the three salinities examined statistically; median lethal temperature for individuals exposed to 25ppt ranged from 36.4 to 37.7°C, 35ppt ranged from 36.4 to 37.7°C, and 45ppt ranged from 36.1 to 37.4°C. Further, LT50 data obtained here for early-juvenile red drum did not differ from data of a similar experiment examining 25mm TL sized fish. Published by Elsevier Ltd.

Gold-bearing hedenbergite skarns from the SW contact of the Andorra granite (Central Pyrenees, Spain)

NASA Astrophysics Data System (ADS)

Soler, A.; Ayora, C.; Cardellach, E.; Delgado, J.

1990-12-01

Several varieties of skarn outcrop have been found to develop along the contact between the Andorra granite and the Devonian limestones. The skarns contain variable amounts of gold ranging up to 5 g/t, always associated with sulphides. The sulphides in the skarn include arsenopyrite and pyrrhotite with lesser amounts of chalcopyrite, galena, sphalerite and Bi-minerals. Geothermometric and geobarometric data indicate the skarns formed at about 2 kbar and temperatures ranging from 500 ° to 350 °C from CO2-free polysaline brines at a slightly acidic pH and oxygen fugacity which decreases with time from the pyrite-pyrrhotite-magnetite towards the QFM buffer. Available data on gold solubility suggest that sufficient quantities of gold to form an ore deposit could have been transported as AuCl{2/-}at the high temperatures and salinities under which the skarns formed. Both gold deposition and sulphide precipitation could have occurred due to a decrease in temperature and/or oxygen fugacity.

Oxidative stress tolerance in intertidal red seaweed Hypnea musciformis (Wulfen) in relation to environmental components.

PubMed

Maharana, Dusmant; Das, Priya Brata; Verlecar, Xivanand N; Pise, Navnath M; Gauns, Manguesh

2015-12-01

Oxidative stress parameters in relation to temperature and other factors have been analysed in Hypnea musciformis, the red seaweed from Anjuna beach, Goa, with an aim to understand its susceptibility to the changing seasons. The results indicate that elevated temperature, sunshine and dessication during peak summer in May enhanced the activity of lipid peroxide, hydrogen peroxide and antioxidants such as catalase, glutathione and ascorbic acid. Statistical tests using multivariate analysis of variance and correlation analysis showed that oxidative stress and antioxidants maintain significant relation with temperature, salinity, sunshine and pH at an individual or interactive level. The dissolved nitrates, phosphates and biological oxygen demand in ambient waters and the trace metals in seaweeds maintained sufficiently low values to provide any indication that could exert contaminant oxidative stress responses. The present field studies suggest that elevated antioxidant content in H. musciformis offer sufficient relief to sustain against harsh environmental stresses for its colonization in the rocky intertidal zone.

Remote Sensing of Salinity: The Dielectric Constant of Sea Water

NASA Technical Reports Server (NTRS)

LeVine, David M.; Lang, R.; Utku, C.; Tarkocin, Y.

2011-01-01

Global monitoring of sea surface salinity from space requires an accurate model for the dielectric constant of sea water as a function of salinity and temperature to characterize the emissivity of the surface. Measurements are being made at 1.413 GHz, the center frequency of the Aquarius radiometers, using a resonant cavity and the perturbation method. The cavity is operated in a transmission mode and immersed in a liquid bath to control temperature. Multiple measurements are made at each temperature and salinity. Error budgets indicate a relative accuracy for both real and imaginary parts of the dielectric constant of about 1%.

Osmolality and respiratory regulation in humans: respiratory compensation for hyperchloremic metabolic acidosis is absent after infusion of hypertonic saline in healthy volunteers.

PubMed

Moen, Vibeke; Brudin, Lars; Rundgren, Mats; Irestedt, Lars

2014-10-01

Several animal studies show that changes in plasma osmolality may influence ventilation. Respiratory depression caused by increased plasma osmolality is interpreted as inhibition of water-dependent thermoregulation because conservation of body fluid predominates at the cost of increased core temperature. Respiratory alkalosis, on the other hand, is associated with a decrease in plasma osmolality and strong ion difference (SID) during human pregnancy. We investigated the hypothesis that osmolality would influence ventilation, so that increased osmolality will decrease ventilation and decreased osmolality will stimulate ventilation in both men and women. Our study participants were healthy volunteers of both sexes (ASA physical status I). Ten men (mean 28 years; range 20-40) and 9 women (mean 33 years; range 22-43) were included. All women participated in both the follicular and luteal phases of the menstrual cycle. Hyperosmolality was induced by IV infusion of hypertonic saline 3%, and hypoosmolality by drinking tap water. Arterial blood samples were collected for analysis of electrolytes, osmolality, and blood gases. Sensitivity to CO2 was determined by rebreathing tests performed before and after the fluid-loading procedures. Infusion of hypertonic saline caused hyperchloremic metabolic acidosis with decreased SID in all subjects. Analysis of pooled data showed absence of respiratory compensation. Baseline arterial PCO2 (PaCO2) mean (SD) 37.8 (2.9) mm Hg remained unaltered, with lowest PaCO2 37.8 (2.9) mm Hg after 100 minutes, P = 0.70, causing a decrease in pH from mean (SD) 7.42 (0.02) to 7.38 (0.02), P < 0.001. Metabolic acidosis was also observed during water loading. Pooled results show that PaCO2 decreased from 38.2 (3.3) mm Hg at baseline to 35.7 (2.8) mm Hg after 80 minutes of drinking water, P = 0.002, and pH remained unaltered: pH 7.43 (0.02) at baseline to pH 7.42 (0.02), P = 0.14, mean difference (confidence interval) = pH -0.007 (-0.017 to 0.003). Our results indicate that osmolality has an influence on ventilation. Respiratory compensation for hyperchloremic metabolic acidosis was suppressed during hyperosmolality. Water loading caused a decrease in plasma osmolality and metabolic acidosis, and although the decrease in SID was smaller compared with salt loading, the expected respiratory compensation was observed. Ventilation was also stimulated in men, therefore independently of progesterone levels. We propose that the influence of osmolality on ventilation consists mainly as depression in conditions of hyperosmolality and that this depression is absent during hypoosmolality.

Accurate Measurements of the Dielectric Constant of Seawater at L Band

NASA Technical Reports Server (NTRS)

Lang, Roger; Zhou, Yiwen; Utku, Cuneyt; Le Vine, David

2016-01-01

This paper describes measurements of the dielectric constant of seawater at a frequency of 1.413 GHz, the center of the protected band (i.e., passive use only) used in the measurement of sea surface salinity from space. The objective of the measurements is to accurately determine the complex dielectric constant of seawater as a function of salinity and temperature. A resonant cylindrical microwave cavity in transmission mode has been employed to make the measurements. The measurements are made using standard seawater at salinities of 30, 33, 35, and 38 practical salinity units over a range of temperatures from 0 degree C to 35 degree C in 5 degree C intervals. Repeated measurements have been made at each temperature and salinity. Mean values and standard deviations are then computed. The total error budget indicates that the real and imaginary parts of the dielectric constant have a combined standard uncertainty of about 0.3 over the range of salinities and temperatures considered. The measurements are compared with the dielectric constants obtained from the model functions of Klein and Swift and those of Meissner and Wentz. The biggest differences occur at low and high temperatures.

Temperature Data Assimilation with Salinity Corrections: Validation for the NSIPP Ocean Data Assimilation System in the Tropical Pacific Ocean, 1993-1998

NASA Technical Reports Server (NTRS)

Troccoli, Alberto; Rienecker, Michele M.; Keppenne, Christian L.; Johnson, Gregory C.

2003-01-01

The NASA Seasonal-to-Interannual Prediction Project (NSIPP) has developed an Ocean data assimilation system to initialize the quasi-isopycnal ocean model used in our experimental coupled-model forecast system. Initial tests of the system have focused on the assimilation of temperature profiles in an optimal interpolation framework. It is now recognized that correction of temperature only often introduces spurious water masses. The resulting density distribution can be statically unstable and also have a detrimental impact on the velocity distribution. Several simple schemes have been developed to try to correct these deficiencies. Here the salinity field is corrected by using a scheme which assumes that the temperature-salinity relationship of the model background is preserved during the assimilation. The scheme was first introduced for a zlevel model by Troccoli and Haines (1999). A large set of subsurface observations of salinity and temperature is used to cross-validate two data assimilation experiments run for the 6-year period 1993-1998. In these two experiments only subsurface temperature observations are used, but in one case the salinity field is also updated whenever temperature observations are available.

Setting accelerated dissolution test for PLGA microspheres containing peptide, investigation of critical parameters affecting drug release rate and mechanism.

PubMed

Tomic, I; Vidis-Millward, A; Mueller-Zsigmondy, M; Cardot, J-M

2016-05-30

The objective of this study was development of accelerated in vitro release method for peptide loaded PLGA microspheres using flow-through apparatus and assessment of the effect of dissolution parameters (pH, temperature, medium composition) on drug release rate and mechanism. Accelerated release conditions were set as pH 2 and 45°C, in phosphate buffer saline (PBS) 0.02M. When the pH was changed from 2 to 4, diffusion controlled phases (burst and lag) were not affected, while release rate during erosion phase decreased two-fold due to slower ester bonds hydrolyses. Decreasing temperature from 45°C to 40°C, release rate showed three-fold deceleration without significant change in release mechanism. Effect of medium composition on drug release was tested in PBS 0.01M (200 mOsm/kg) and PBS 0.01M with glucose (380 mOsm/kg). Buffer concentration significantly affected drug release rate and mechanism due to the change in osmotic pressure, while ionic strength did not have any effect on peptide release. Furthermore, dialysis sac and sample-and-separate techniques were used, in order to evaluate significance of dissolution technique choice on the release process. After fitting obtained data to different mathematical models, flow-through method was confirmed as the most appropriate for accelerated in vitro dissolution testing for a given formulation. Copyright © 2016 Elsevier B.V. All rights reserved.

Tistlia consotensis gen. nov., sp. nov., an aerobic, chemoheterotrophic, free-living, nitrogen-fixing alphaproteobacterium, isolated from a Colombian saline spring.

PubMed

Díaz-Cárdenas, C; Patel, B K C; Baena, S

2010-06-01

A Gram-negative, aerobic, mesophilic, non-spore-forming, chemotrophic, chlorophyll-lacking, nitrogen-fixing bacterium, designated strain USBA 355(T), was isolated from the saline spring 'Salado de Consotá' situated in the Colombian Andes. The non-flagellated cells of strain USBA 355(T) were straight to slightly curved rods (0.6-0.7 x 3.0-3.5 microm). Growth occurred optimally at 30 degrees C (growth temperature range between 20 and 40 degrees C), at pH 6.5-6.7 (pH growth range between 5.0 and 8.0) and at 0.5 % NaCl (w/v) (range between 0 and 4 %). The major quinone present was Q-10 and the predominant fatty acids identified were C(19 : 0) cyclo omega8c, C(18 : 1)omega7c and C(18 : 0). The G+C content of the chromosomal DNA was 71+/-1 mol%. 16S rRNA gene sequence analysis indicated that strain USBA 355(T) formed a distant phylogenetic line of descent with members of the genus Thalassobaculum, family Rhodospirillaceae, class Alphaproteobacteria (90 % gene sequence similarity). Comparison of the phylogenetic, chemotaxonomic and physiological features of strain USBA 355(T) with all other members of the family Rhodospirillaceae suggested that it represents a novel genus and species for which the name Tistlia consotensis gen. nov., sp. nov. is proposed. The type strain of the type species is USBA 355(T) (=JCM 15529(T)=KCTC 22406(T)).

FerryBox and MERIS Assessment of coastal and shelf sea ecosystems by combining in situ and remotely sensed data

NASA Astrophysics Data System (ADS)

Petersen, W.; Wehde, H.; Krasemann, H.; Colijn, F.; Schroeder, F.

2008-04-01

An automatic measuring system called " FerryBox" was installed in the North Sea on a ferry travelling between Germany (Cuxhaven) and Great Britain (Harwich), enabling online oceanographic and biological measurements such as salinity, temperature, fluorescence, turbidity, oxygen, pH, and nutrient concentrations. Observations made along the ferry transect reveal characteristic phenomena such as high salinity inflow through the Channel into the Southern Bight, algal bloom dynamics and related oxygen and pH changes. Combination of these online observations with remote sensing enhances the spatial resolution of the transect related measurements. Several examples of the synergy between these two measuring strategies are shown, both for large-scale algal blooms in the North Sea as well as for local intense but short-term blooms in the German Bight. Coherence of the data sets can be gained and improved by using water transport models in order to obtain synoptic overviews of the remotely sensed and FerryBox related parameters. Limitations of the currently used algorithms for deriving chlorophyll- a from remote sensing images for coastal and shelf seas (Case-2 water) are discussed, as well as depth related processes which cannot be properly resolved on the basis of water intake at a fixed point. However, in unstratified coastal waters under normal conditions FerryBox data represent average conditions. The importance of future applications of this combination of methods for monitoring of coastal waters is emphasized.

Surface water characteristics and trace metals level of the Bonny/New Calabar River Estuary, Niger Delta, Nigeria

NASA Astrophysics Data System (ADS)

Onojake, M. C.; Sikoki, F. D.; Omokheyeke, O.; Akpiri, R. U.

2017-05-01

Surface water samples from three stations in the Bonny/New Calabar River Estuary were analyzed for the physicochemical characteristics and trace metal level in 2011 and 2012, respectively. Results show pH ranged from 7.56 to 7.88 mg/L; conductivity, 33,489.00 to 33,592.00 µScm-1; salinity, 15.33 to 15.50 ‰; turbidity, 4.35 to 6.65 NTU; total dissolved solids, 22111.00 to 23263.00 gm-3; dissolved oxygen, 4.53 to 6.65 mg/L; and biochemical oxygen demand, 1.72 mg/L. The level of some trace metals (Ca, Mg, K, Zn, Pb, Cd, Co, Cr, Cu, Fe, Ni, and Na) were also analyzed by Atomic absorption spectrometry with K, Zn, and Co being statistically significant ( P < 0.05). The results were compared with USEPA and WHO Permissible Limits for water quality standards. It was observed that the water quality parameters in the Bonny Estuary show seasonal variation with higher values for pH, DO, BOD, temperature, and salinity during the dry season than wet season. Concentrations of trace metals such as Pb, Cd, Zn, Ni, and Cr were higher than stipulated limits by WHO (2006). The result of the Metal Pollution Index suggests that the river was slightly affected and therefore continuous monitoring is necessary to avert possible public health implications of these metals on consumers of water and seafood from the study area.

Sea Surface Scanner: An advanced catamaran to study the sea surface

NASA Astrophysics Data System (ADS)

Wurl, O.; Mustaffa, N. I. H.; Ribas Ribas, M.

2016-02-01

The Sea Surface Scanner is a remote-controlled catamaran with the capability to sample the sea-surface microlayer in high resolution. The catamaran is equipped with a suite of sensors to scan the sea surface on chemical, biological and physical parameters. Parameters include UV absorption, fluorescence spectra, chlorophyll-a, photosynthetic efficiency, chromophoric dissolved organic matter (CDOM), dissolved oxygen, pH, temperature, and salinity. A further feature is a capability to collect remotely discrete water samples for detailed lab analysis. We present the first high-resolution (< 30 sec) data on the sea surface microlayer. We discuss the variability of biochemical properties of the sea surface and its implication on air-sea interaction.

Remote sensing of salinity

NASA Technical Reports Server (NTRS)

Thomann, G. C.

1975-01-01

The complex dielectric constant of sea water is a function of salinity at 21 cm wavelength, and sea water salinity can be determined by a measurement of emissivity at 21 cm along with a measurement of thermodynamic temperature. Three aircraft and one helicopter experiments using two different 21 cm radiometers were conducted under different salinity and temperature conditions. Single or multiple ground truth measurements were used to calibrate the data in each experiment. It is inferred from these experiments that accuracies of 1 to 2%/OO are possible with a single surface calibration point necessary only every two hours if the following conditions are met--water temperatures above 20 C, salinities above 10%/OO, and level plane flight. More frequent calibration, constraint of the aircraft's orientation to the same as it was during calibration, and two point calibration (at a high and low salinity level) rather than single point calibration may give even better accuracies in some instances.

Abiotic versus biotic drivers of ocean pH variation under fast sea ice in McMurdo Sound, Antarctica.

PubMed

Matson, Paul G; Washburn, Libe; Martz, Todd R; Hofmann, Gretchen E

2014-01-01

Ocean acidification is expected to have a major effect on the marine carbonate system over the next century, particularly in high latitude seas. Less appreciated is natural environmental variation within these systems, particularly in terms of pH, and how this natural variation may inform laboratory experiments. In this study, we deployed sensor-equipped moorings at 20 m depths at three locations in McMurdo Sound, comprising deep (bottom depth>200 m: Hut Point Peninsula) and shallow environments (bottom depth ∼25 m: Cape Evans and New Harbor). Our sensors recorded high-frequency variation in pH (Hut Point and Cape Evans only), tide (Cape Evans and New Harbor), and water mass properties (temperature and salinity) during spring and early summer 2011. These collective observations showed that (1) pH differed spatially both in terms of mean pH (Cape Evans: 8.009±0.015; Hut Point: 8.020±0.007) and range of pH (Cape Evans: 0.090; Hut Point: 0.036), and (2) pH was not related to the mixing of two water masses, suggesting that the observed pH variation is likely not driven by this abiotic process. Given the large daily fluctuation in pH at Cape Evans, we developed a simple mechanistic model to explore the potential for biotic processes--in this case algal photosynthesis--to increase pH by fixing carbon from the water column. For this model, we incorporated published photosynthetic parameters for the three dominant algal functional groups found at Cape Evans (benthic fleshy red macroalgae, crustose coralline algae, and sea ice algal communities) to estimate oxygen produced/carbon fixed from the water column underneath fast sea ice and the resulting pH change. These results suggest that biotic processes may be a primary driver of pH variation observed under fast sea ice at Cape Evans and potentially at other shallow sites in McMurdo Sound.

Abiotic versus Biotic Drivers of Ocean pH Variation under Fast Sea Ice in McMurdo Sound, Antarctica

PubMed Central

Matson, Paul G.; Washburn, Libe; Martz, Todd R.; Hofmann, Gretchen E.

2014-01-01

Ocean acidification is expected to have a major effect on the marine carbonate system over the next century, particularly in high latitude seas. Less appreciated is natural environmental variation within these systems, particularly in terms of pH, and how this natural variation may inform laboratory experiments. In this study, we deployed sensor-equipped moorings at 20 m depths at three locations in McMurdo Sound, comprising deep (bottom depth>200 m: Hut Point Peninsula) and shallow environments (bottom depth ∼25 m: Cape Evans and New Harbor). Our sensors recorded high-frequency variation in pH (Hut Point and Cape Evans only), tide (Cape Evans and New Harbor), and water mass properties (temperature and salinity) during spring and early summer 2011. These collective observations showed that (1) pH differed spatially both in terms of mean pH (Cape Evans: 8.009±0.015; Hut Point: 8.020±0.007) and range of pH (Cape Evans: 0.090; Hut Point: 0.036), and (2) pH was not related to the mixing of two water masses, suggesting that the observed pH variation is likely not driven by this abiotic process. Given the large daily fluctuation in pH at Cape Evans, we developed a simple mechanistic model to explore the potential for biotic processes – in this case algal photosynthesis – to increase pH by fixing carbon from the water column. For this model, we incorporated published photosynthetic parameters for the three dominant algal functional groups found at Cape Evans (benthic fleshy red macroalgae, crustose coralline algae, and sea ice algal communities) to estimate oxygen produced/carbon fixed from the water column underneath fast sea ice and the resulting pH change. These results suggest that biotic processes may be a primary driver of pH variation observed under fast sea ice at Cape Evans and potentially at other shallow sites in McMurdo Sound. PMID:25221950

Relationship in between Chemical Oxidation and Browning of Flavanols

NASA Astrophysics Data System (ADS)

Dong, X.; Zhang, Y. L.; Wang, F.; Pang, M. X.; Qi, J. H.

2016-08-01

Catechin, epicatechin and chlorogenic acid are widely distributed in the plant kingdom. At present, influencing factors of phenol chemical oxidation is little research. In order to study non-enzymatic browning factors, this research utilized catechin, epicatechin and chlorogenic acid to establish simulation systems. The browning degree and products of flavanols were investigated by transmittance and high-performance liquid chromatography (HPLC). The main results and conclusions were follows. The brown generation is increased after phenols of chemical oxidation at 50°C and at pH 3.7 phosphate buffered saline, the sequence of influencing factor of browning is pH > kind of phenol > temperature. Oxidation of compounds of catechin and epicatechin results in formation of their Methylene quinone or o -Quinones. In addition, oxidation products of catechin, epicatechin and chlorogenic acid were mixture of different molecular sizes. The research has showed that brown generation correlated well with chemical oxidation of phenols and chemical oxidation reaction generated larger molecular weight polymers.

Technical Note: Artificial coral reef mesocosms for ocean acidification investigations

NASA Astrophysics Data System (ADS)

Leblud, J.; Moulin, L.; Batigny, A.; Dubois, P.; Grosjean, P.

2014-11-01

The design and evaluation of replicated artificial mesocosms are presented in the context of a thirteen month experiment on the effects of ocean acidification on tropical coral reefs. They are defined here as (semi)-closed (i.e. with or without water change from the reef) mesocosms in the laboratory with a more realistic physico-chemical environment than microcosms. Important physico-chemical parameters (i.e. pH, pO2, pCO2, total alkalinity, temperature, salinity, total alkaline earth metals and nutrients availability) were successfully monitored and controlled. Daily variations of irradiance and pH were applied to approach field conditions. Results highlighted that it was possible to maintain realistic physico-chemical parameters, including daily changes, into artificial mesocosms. On the other hand, the two identical artificial mesocosms evolved differently in terms of global community oxygen budgets although the initial biological communities and physico-chemical parameters were comparable. Artificial reef mesocosms seem to leave enough degrees of freedom to the enclosed community of living organisms to organize and change along possibly diverging pathways.

Effects of critical medium components on the production of antifungal lipopeptides from Bacillus amyloliquefaciens Q-426 exhibiting excellent biosurfactant properties.

PubMed

Zhao, Pengchao; Quan, Chunshan; Jin, Liming; Wang, Lina; Wang, Jianhua; Fan, Shengdi

2013-03-01

In this study, influence of three critical parameters nitrogen sources, initial pH and metal ions was discussed in the production of antifungal lipopeptides from Bacillus amyloliquefaciens Q-426. The results revealed that lipopeptide biosynthesis might have relations with the population density of strain Q-426 and some special amino acids. Also, the alkali-resistant strain Q-426 could grow well in the presence of Fe(2+) ions below 0.8 M l(-1) and still maintain the competitive advantage below 0.2 M l(-1). Moreover, lipopeptides exhibited significant inhibitory activities against Curvularia lunata (Walk) Boed even at the extreme conditions of temperature, pH and salinity. Finally, biosurfactant properties of lipopeptides mixture were evaluated by use with totally six different methods including bacterial adhesion to hydrocarbons assay, lipase activity, hemolytic activity, emulsification activity, oil displacement test and surface tension measurement. The research suggested that B. amyloliquefaciens Q-426 may have great potential in agricultural and environmental fields.

Physicochemical studies on Uburu Salt Lake Ebonyi State-Nigeria.

PubMed

Akubugwo, I E; Ofoegbu, C J; Ukwuoma, C U

2007-09-15

Physicochemical properties of soil (sediment) and water from Uburu salt lake were evaluated and compared with control soil and surface water from the same community. Results showed significant (p < 0.05) higher values for the heavy metals cadmium, chromium, copper, lead and zinc in the lake water relative to the control. The values of these metals in the lake soil (sediments) however, were significantly (p < 0.05) lower than the control soil. Similar significant (p < 0.05) elevations were observed in the lake water temperature, salinity, pH, calcium, magnesium, sodium, potassium, nitrate, carbonate, sulphate and phosphate levels compared to the control. Significant (p < 0.05) changes were also noted in the lake soil's pH, exchangeable acidity, nitrogen, organic carbon, calcium and magnesium levels. Also the soil texture was affected relative to the control. In a number of cases, the values of the studied parameters were higher than the permissible WHO standards. In view of these findings, cautious use of the salt lake soil and water is advocated.

Effects of chemical elements in the trophic levels of natural salt marshes.

PubMed

Kamiński, Piotr; Barczak, Tadeusz; Bennewicz, Janina; Jerzak, Leszek; Bogdzińska, Maria; Aleksandrowicz, Oleg; Koim-Puchowska, Beata; Szady-Grad, Małgorzata; Klawe, Jacek J; Woźniak, Alina

2016-06-01

The relationships between the bioaccumulation of Na, K, Ca, Mg, Fe, Zn, Cu, Mn, Co, Cd, and Pb, acidity (pH), salinity (Ec), and organic matter content within trophic levels (water-soil-plants-invertebrates) were studied in saline environments in Poland. Environments included sodium manufactures, wastes utilization areas, dumping grounds, and agriculture cultivation, where disturbed Ca, Mg, and Fe exist and the impact of Cd and Pb is high. We found Zn, Cu, Mn, Co, and Cd accumulation in the leaves of plants and in invertebrates. Our aim was to determine the selectivity exhibited by soil for nutrients and heavy metals and to estimate whether it is important in elucidating how these metals are available for plant/animal uptake in addition to their mobility and stability within soils. We examined four ecological plant groups: trees, shrubs, minor green plants, and water macrophytes. Among invertebrates, we sampled breastplates Malacostraca, small arachnids Arachnida, diplopods Diplopoda, small insects Insecta, and snails Gastropoda. A higher level of chemical elements was found in saline polluted areas (sodium manufactures and anthropogenic sites). Soil acidity and salinity determined the bioaccumulation of free radicals in the trophic levels measured. A pH decrease caused Zn and Cd to increase in sodium manufactures and an increase in Ca, Zn, Cu, Cd, and Pb in the anthropogenic sites. pH increase also caused Na, Mg, and Fe to increase in sodium manufactures and an increase in Na, Fe, Mn, and Co in the anthropogenic sites. There was a significant correlation between these chemical elements and Ec in soils. We found significant relationships between pH and Ec, which were positive in saline areas of sodium manufactures and negative in the anthropogenic and control sites. These dependencies testify that the measurement of the selectivity of cations and their fluctuation in soils provide essential information on the affinity and binding strength in these environments. The chemical elements accumulated in soils and plants; however, further flow is selective and variable. The selectivity exhibited by soil systems for nutrients and heavy metals is important in elucidating how these metals become available for plant/animal uptake and also their mobility and stability in soils.

Effects of temperature and salinity on larval survival and development in the invasive shrimp Palaemon macrodactylus (Caridea: Palaemonidae) along the reproductive season

NASA Astrophysics Data System (ADS)

Guadalupe Vázquez, M.; Bas, Claudia C.; Kittlein, Marcelo; Spivak, Eduardo D.

2015-05-01

The invasive shrimp Palaemon macrodactylus is associated mainly with brackish waters. Previous studies raised the question if tolerance to low salinities differs between larvae and adults. To answer this question, the combined effects of two temperatures (20 and 25 °C) and four salinities (5, 12, 23 and 34 psu) on survival and development of larvae that hatched at the beginning, in the midpoint and near the end of a reproductive season (denoted early, middle season and late larvae respectively) were examined. The three types of larvae were able to survive and reach juvenile phase at salinities between 12 and 34 psu and at both temperatures. At 5 psu all larvae died, but 45% molted at least once. Temperature and salinity to a lesser extent, had effects on the duration of development and on the number of larval stages in all larval types. Development was longer at the lower temperature, especially in middle season and late larvae. Most early larvae reached the juvenile phase through 5 larval stages; the number of larval stages of middle season and late larvae was higher at 20 °C and in late larvae also low salinity produced extra stages. Low salinity (12 psu) and, in early and middle season larvae, low temperature produced lighter and smaller individuals. Response of larvae to environmental factors seems to be related in part to the previous conditions (maternal effects and/or embryo development conditions). The narrower salinity tolerance of larvae compared to adults and the ability of zoea I to survive at least some days at 5 psu may be related with an export larval strategy.

Upper-Ocean Variability in the Arctic’s Amundsen and Nansen Basins

DTIC Science & Technology

2017-05-01

collect vertical profiles of ocean temperature, salinity and horizontal velocity at few- hour interval as well as sample for specified time periods...deployed for the MIZ program - specifically, vertical temperature, salinity and velocity profiles were collected every 3 hours in the upper 250m of the...the system), this ITP-V returned 5+ months of upper ocean temperature, salinity , velocity and turbulence data from the Makarov Basin, a region of

Impacts of blending ground, surface, and saline waters on lead release in drinking water distribution systems.

PubMed

Tang, Zhijian; Hong, Seungkwan; Xiao, Weizhong; Taylor, James

2006-03-01

The impacts of distribution water quality changes caused by blending different source waters on lead release from corrosion loops containing small lead coupons were investigated in a pilot distribution study. The 1-year pilot study demonstrated that lead release to drinking water increased as chlorides increased and sulfates decreased. Silica and calcium inhibited lead release to a lesser degree than sulfates. An additional 3-month field study isolated and verified the effects of chlorides and sulfates on lead release. Lead release decreased with increasing pH and increasing alkalinity during the 1-year pilot study; however, the effects of pH and alkalinity on lead release, were not clearly elucidated due to confounding effects. A statistical model was developed using nonlinear regression, which showed that lead release increased with increasing chlorides, alkalinity and temperature, and decreased with increasing pH and sulfates. The model indicated that primary treatment processes such as enhanced coagulation and RO (reverse osmosis membrane) were related to lead release by water quality. Chlorides are high in RO-finished water and increase lead release, while sulfates are high following enhanced coagulation and decrease lead release.

Marine bivalve shell geochemistry and ultrastructure from modern low pH environments: environmental effect versus experimental bias

NASA Astrophysics Data System (ADS)

Hahn, S.; Rodolfo-Metalpa, R.; Griesshaber, E.; Schmahl, W. W.; Buhl, D.; Hall-Spencer, J. M.; Baggini, C.; Fehr, K. T.; Immenhauser, A.

2012-05-01

Bivalve shells can provide excellent archives of past environmental change but have not been used to interpret ocean acidification events. We investigated carbon, oxygen and trace element records from different shell layers in the mussels Mytilus galloprovincialis combined with detailed investigations of the shell ultrastructure. Mussels from the harbour of Ischia (Mediterranean, Italy) were transplanted and grown in water with mean pHT 7.3 and mean pHT 8.1 near CO2 vents on the east coast of the island. Most prominently, the shells recorded the shock of transplantation, both in their shell ultrastructure, textural and geochemical record. Shell calcite, precipitated subsequently under acidified seawater responded to the pH gradient by an in part disturbed ultrastructure. Geochemical data from all test sites show a strong metabolic effect that exceeds the influence of the low-pH environment. These field experiments showed that care is needed when interpreting potential ocean acidification signals because various parameters affect shell chemistry and ultrastructure. Besides metabolic processes, seawater pH, factors such as salinity, water temperature, food availability and population density all affect the biogenic carbonate shell archive.

Oxygen and carbon dioxide in the marine intertidal environment: diurnal and tidal changes in rockpools.

PubMed

Truchot, J P; Duhamel-Jouve, A

1980-03-01

Water oxygen partial pressure (PO2), pH, titration alkalinity (TA), temperature and salinity were measured hourly in rockpools during emersion periods occuring at various times of the diurnal cycle. Measurements allowed calculation of oxygen concentration (CO2), CO2 partial pressure (PCO2) and concentrations of bicarbonate, carbonate and total CO2 (CCO2). During night emersion periods, water PO2 decreased to almost zero in a few hours, pH fell, TA rose and PCO2 increased up to 1-3 Torr. During day emersion periods, water PO2 rose to 400-600 Torr, pH increased to more than 10, TA decreased substantially and PCO2 fell as low as 10(-4) Torr. The direction of the observed changes depended essentially on the illumination, indicating that respiratory and photosynthetic activities were the main processes involved. The large variations of the components of the carbonate system imply considerable changes of the CO2 capacitance coefficient in water, mainly during the day-time emersion. These changes are discussed in relation to the respiratory and acid-base physiology of the animals living in these biotopes.

Magnetic Resonance Imaging of Electrolysis.

PubMed Central

Meir, Arie; Hjouj, Mohammad; Rubinsky, Liel; Rubinsky, Boris

2015-01-01

This study explores the hypothesis that Magnetic Resonance Imaging (MRI) can image the process of electrolysis by detecting pH fronts. The study has relevance to real time control of cell ablation with electrolysis. To investigate the hypothesis we compare the following MR imaging sequences: T1 weighted, T2 weighted and Proton Density (PD), with optical images acquired using pH-sensitive dyes embedded in a physiological saline agar solution phantom treated with electrolysis and discrete measurements with a pH microprobe. We further demonstrate the biological relevance of our work using a bacterial E. Coli model, grown on the phantom. The results demonstrate the ability of MRI to image electrolysis produced pH changes in a physiological saline phantom and show that these changes correlate with cell death in the E. Coli model grown on the phantom. The results are promising and invite further experimental research. PMID:25659942

Environmental Monitoring of Microbe Metabolic Transformation

NASA Technical Reports Server (NTRS)

Bebout, Brad (Inventor); Fleming, Erich (Inventor); Piccini, Matthew (Inventor); Beasley, Christopher (Inventor); Bebout, Leslie (Inventor)

2013-01-01

Mobile system and method for monitoring environmental parameters involved in growth or metabolic transformation of algae in a liquid. Each of one or more mobile apparati, suspended or partly or wholly submerged in the liquid, includes at least first and second environmental sensors that sense and transmit distinct first and second environmental, growth or transformation parameter values, such as liquid temperature, temperature of gas adjacent to and above the exposed surface, liquid pH, liquid salinity, liquid turbidity, O.sub.2 dissolved in the liquid, CO.sub.2 contained in the liquid, oxidization and reduction potential of the liquid, nutrient concentrations in the liquid, nitrate concentration in the liquid, ammonium concentration in the liquid, bicarbonate concentration in the liquid, phosphate concentration in the liquid, light intensity at the liquid surface, electrical conductivity of the liquid, and a parameter.alpha.(alga) associated with growth stage of the alga, using PAM fluorometry or other suitable parameter measurements.

Naturally occurring bacteraemia in American lobsters, Homarus americanus Milne-Edwards, in Long Island Sound.

PubMed

Bartlett, S L; Wooster, G A; Sokolowski, M S; Dove, A D M; Bowser, P R

2008-01-01

The health status of the American lobster, Homarus americanus Milne-Edwards, in Long Island Sound (LIS) has been in decline, with seasonal mortality events occurring since 1998. In order to assess the potential effects of environmental conditions on lobster health via haemolymph analysis, lobsters collected from various sites in LIS were examined and sampled while concurrent environmental data (water temperature, salinity and dissolved oxygen) were recorded. The pH of the haemolymph of each lobster was tested, followed by a collection of haemolymph for serum biochemistry analysis and bacterial culture. This report focuses on the results of the bacterial sampling. The majority of bacteria cultured were opportunistic pathogens commonly found in the environment, including some that are associated with sewage and pollution. The prevalence of bacteraemia was correlated with the site of collection, the month in which the lobsters were sampled, and water temperature.

Dark production of carbon monoxide (CO) from dissolved organic matter in the St. Lawrence estuarine system: Implication for the global coastal and blue water CO budgets

NASA Astrophysics Data System (ADS)

Zhang, Yong; Xie, Huixiang; Fichot, CéDric G.; Chen, Guohua

2008-12-01

We investigated the thermal (dark) production of carbon monoxide (CO) from dissolved organic matter (DOM) in the water column of the St. Lawrence estuarine system in spring 2007. The production rate, Qco, decreased seaward horizontally and downward vertically. Qco exhibited a positive, linear correlation with the abundance of chromophoric dissolved organic matter (CDOM). Terrestrial DOM was more efficient at producing CO than marine DOM. The temperature dependence of Qco can be characterized by the Arrhenius equation with the activation energies of freshwater samples being higher than those of salty samples. Qco remained relatively constant between pH 4-6, increased slowly between pH 6-8 and then rapidly with further rising pH. Ionic strength and iron chemistry had little influence on Qco. An empirical equation, describing Qco as a function of CDOM abundance, temperature, pH, and salinity, was established to evaluate CO dark production in the global coastal waters (depth < 200 m). The total coastal CO dark production from DOM was estimated to be from 0.46 to 1.50 Tg CO-C a-1 (Tg carbon from CO a-1). We speculated the global oceanic (coastal plus open ocean) CO dark production to be in the range from 4.87 to 15.8 Tg CO-C a-1 by extrapolating the coastal water-based results to blue waters (depth > 200 m). Both the coastal and global dark source strengths are significant compared to the corresponding photochemical CO source strengths (coastal: ˜2.9 Tg CO-C a-1; global: ˜50 Tg CO-C a-1). Steady state deepwater CO concentrations inferred from Qco and microbial CO uptake rates are <0.1 nmol L-1.

The salinity, temperature, and delta18O of the glacial deep ocean.

PubMed

Adkins, Jess F; McIntyre, Katherine; Schrag, Daniel P

2002-11-29

We use pore fluid measurements of the chloride concentration and the oxygen isotopic composition from Ocean Drilling Program cores to reconstruct salinity and temperature of the deep ocean during the Last Glacial Maximum (LGM). Our data show that the temperatures of the deep Pacific, Southern, and Atlantic oceans during the LGM were relatively homogeneous and within error of the freezing point of seawater at the ocean's surface. Our chloride data show that the glacial stratification was dominated by salinity variations, in contrast with the modern ocean, for which temperature plays a primary role. During the LGM the Southern Ocean contained the saltiest water in the deep ocean. This reversal of the modern salinity contrast between the North and South Atlantic implies that the freshwater budget at the poles must have been quite different. A strict conversion of mean salinity at the LGM to equivalent sea-level change yields a value in excess of 140 meters. However, the storage of fresh water in ice shelves and/or groundwater reserves implies that glacial salinity is a poor predictor of mean sea level.

Effects of Salinity and Temperature on Growth and Survival of Juvenile Iwagaki Oyster Crassostrea nippona

NASA Astrophysics Data System (ADS)

Wang, Tao; Li, Qi

2018-03-01

Iwagaki oyster Crassostrea nippona occurs naturally along the coasts of Japan and Korea. Because of its unique flavor, delicious taste, edibility during the summer and high commercial value, it has been identified as a potential aquaculture species. To determine the optimum aquaculture conditions and provide necessary information for mass production of the juvenile, the effects of six salinities (15, 20, 25, 30, 35 and 40) and five temperatures (16, 20, 24, 28 and 32₿ on growth and survival of juvenile C. nippona were examined in this study. In the salinity experiment, the largest values of mean shell height and growth rate were observed at salinity 25 (20.96 ± 0.36 mm and 172.0 μm d↿, respectively), which were significantly different (P < 0.05) with those of other treatments, except at salinity 30 (20.56 ± 1.05 mm and 160.3 μm d↿, respectively) (P > 0.05). The maximum survival rate 84.44% was always observed at salinity 20, and there was no significant difference (P > 0.05) in survival rate among salinities varying between 15 and 35. In the temperature-related experiments, the highest growth and survival rates of juvenile were observed at 24₿(180.8 μm d↿ and 84.4%) and 28₿(190.7 μm d↿ and 83.3%), respectively, on day 20, and showed significantly (P < 0.05) larger size and higher survival rate than any other groups. Both juvenile survival and growth were significantly depressed at extreme salinities (15, 40) and temperatures (16₿ 32₿. Based on the results of the present study, a salinity range from 25 to 30 and a temperature range from 24 to 28₿are considered optimal conditions for survival and growth of juvenile C. nippona.

Climate change and soil salinity: The case of coastal Bangladesh.

PubMed

Dasgupta, Susmita; Hossain, Md Moqbul; Huq, Mainul; Wheeler, David

2015-12-01

This paper estimates location-specific soil salinity in coastal Bangladesh for 2050. The analysis was conducted in two stages: First, changes in soil salinity for the period 2001-2009 were assessed using information recorded at 41 soil monitoring stations by the Soil Research Development Institute. Using these data, a spatial econometric model was estimated linking soil salinity with the salinity of nearby rivers, land elevation, temperature, and rainfall. Second, future soil salinity for 69 coastal sub-districts was projected from climate-induced changes in river salinity and projections of rainfall and temperature based on time trends for 20 Bangladesh Meteorological Department weather stations in the coastal region. The findings indicate that climate change poses a major soil salinization risk in coastal Bangladesh. Across 41 monitoring stations, the annual median projected change in soil salinity is 39 % by 2050. Above the median, 25 % of all stations have projected changes of 51 % or higher.

pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas)

PubMed Central

Suquet, Marc; Malo, Florent; Queau, Isabelle; Pignet, Patricia; Ratiskol, Dominique; Le Grand, Jacqueline; Huber, Matthias; Cosson, Jacky

2018-01-01

ABSTRACT Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster, Crassostrea gigas. The acidic pH of the gonad (5.82±0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared to NH4Cl-free SW, revealing the role of pHi in triggering sperm movement. Percentage of motile spermatozoa activated in Na+-free artificial seawater (ASW) was highly reduced compared to ASW, suggesting that change of pHi triggering sperm motility was mediated by a Na+/H+ exchanger. Motility and swimming speed were highest in salinities between 33.8 and 42.7‰ (within a range of 0 to 50 ‰), and pH values above 7.5 (within a range of 4.5 to 9.5). PMID:29483075

Alleviation of the effects of saline-alkaline stress on maize seedlings by regulation of active oxygen metabolism by Trichoderma asperellum.

PubMed

Fu, Jian; Liu, Zhihua; Li, Zuotong; Wang, Yufeng; Yang, Kejun

2017-01-01

This study investigated the influence of Trichoderma asperellum on active oxygen production in maize seedlings under saline-alkaline stress conditions. Two maize cultivars were tested: 'Jiangyu 417' ('JY417'), which can tolerate saline-alkaline stress; and, 'Xianyu 335' ('XY335'), which is sensitive to saline-alkaline stress. The seedlings were grown on natural saline-alkaline soil (pH 9.30) in plastic pots. To each liter of saline-alkaline soil, 200 mL of T. asperellum spore suspension was applied; three fungal suspensions were used, namely, 1 × 103, 1 × 106, and 1 × 109 spores/L. A control with only the vehicle applied was also established, along with a second control in which untreated meadow soil (pH 8.23) was used. Root and leaf samples were collected when the seedlings had three heart-shaped leaves and the fourth was in the developmental phase. Physical and biochemical parameters related to oxidation resistance were assessed. The results indicated that the 'JY417' and 'XY335' seedlings showed different degrees of oxidative damage and differences in their antioxidant defense systems under saline-alkaline stress. As the spore density of the fungal suspension increased, the K+ and Ca2+ contents in the seedlings increased, but Na+ content decreased. Moreover, fungal treatment promoted the synthesis or accumulation of osmolytes, which enhanced the water absorbing capacity of the cells, increased antioxidant enzyme activities, enhanced the content of non-enzyme antioxidants, and reduced the accumulation of reactive oxygen species. Fungal treatment alleviated oxidative damage caused by the saline-alkaline stress in roots and leaves of the seedlings. The application of T. asperellum overcame the inhibitory effect of saline-alkaline soil stress on the growth of maize seedlings. In the present experiment, application with 1 × 109 spores/L gave the optimal results.

A multilevel trait-based approach to the ecological performance of Microcystis aeruginosa complex from headwaters to the ocean.

PubMed

Kruk, Carla; Segura, Angel M; Nogueira, Lucía; Alcántara, Ignacio; Calliari, Danilo; Martínez de la Escalera, Gabriela; Carballo, Carmela; Cabrera, Carolina; Sarthou, Florencia; Scavone, Paola; Piccini, Claudia

2017-12-01

The Microcystis aeruginosa complex (MAC) clusters cosmopolitan and conspicuous harmful bloom-forming cyanobacteria able to produce cyanotoxins. It is hypothesized that low temperatures and brackish salinities are the main barriers to MAC proliferation. Here, patterns at multiple levels of organization irrespective of taxonomic identity (i.e. a trait-based approach) were analyzed. MAC responses from the intracellular (e.g. respiratory activity) to the ecosystem level (e.g. blooms) were evaluated in wide environmental gradients. Experimental results on buoyancy and respiratory activity in response to increased salinity (0-35) and a literature review of maximum growth rates under different temperatures and salinities were combined with field sampling from headwaters (800km upstream) to the marine end of the Rio de la Plata estuary (Uruguay-South America). Salinity and temperature were the major variables affecting MAC responses. Experimentally, freshwater MAC cells remained active for 24h in brackish waters (salinity=15) while colonies increased their flotation velocity. At the population level, maximum growth rate decreased with salinity and presented a unimodal exponential response with temperature, showing an optimum at 27.5°C and a rapid decrease thereafter. At the community and ecosystem levels, MAC occurred from fresh to marine waters (salinity 30) with a sustained relative increase of large mucilaginous colonies biovolume with respect to individual cells. Similarly, total biomass and, specific and morphological richness decreased with salinity while blooms were only detected in freshwater both at high (33°C) and low (11°C) temperatures. In brackish waters, large mucilaginous colonies presented advantages under osmotic restrictive conditions. These traits values have also been associated with higher toxicity potential. This suggest salinity or low temperatures would not represent effective barriers for the survival and transport of potentially toxic MAC under likely near future scenarios of increasing human impacts (i.e. eutrophication, dam construction and climate change). Copyright © 2017 Elsevier B.V. All rights reserved.

Patterns of fish diversity and assemblage structure and water quality in the longest Asian tropical river (Mekong)

NASA Astrophysics Data System (ADS)

Chea, R.; Lek, S.; Grenouillet, G.

2016-12-01

Although the Mekong River is one of the world's 35 biodiversity hotspots, the large-scale patterns of fish diversity and assemblage structure remain poorly addressed. The present study aimed to investigate the spatial variability of water quality in the Lower Mekong Basin and the fish distribution patterns in the Lower Mekong River (LMR) and to identify their environmental determinants. Daily fish catch data at 38 sites distributed along the LMR were related to 15 physicochemical and 19 climatic variables. As a result, four different clusters were defined according to the similarity in assemblage composition and 80 indicator species were identified. While fish species richness was highest in the Mekong delta and lowest in the upper part of the LMR, the diversity index was highest in the middle part of the LMR and lowest in the delta. We found that fish assemblages changed along the environmental gradients and that the main drivers affecting the fish assemblage structure were the seasonal variation of temperature, precipitation, dissolved oxygen, pH, and total phosphorus. Specifically, upstream assemblages were characterized by cyprinids and Pangasius catfish, well suited to low temperature, high dissolved oxygen and high pH. Fish assemblages in the delta were dominated by perch-like fish and clupeids, more tolerant to high temperatures, and high levels of nutrients (nitrates and total phosphorus) and salinity. Overall, the patterns were consistent between seasons. Our study contributes to establishing the first holistic fish community study in the LMR. Overall of the LMR water quality, we found that the water in the mainstream was less polluted than its tributaries; eutrophication and salinity could be key factors affecting water quality in LMR. Moreover, the seasonal variation of water quality seemed to be less marked than spatial variation occurring along the longitudinal gradient of Mekong River. Significant degradations were mainly associated with human disturbance and particularly apparent in sites distributed along the man-made canals in Vietnam delta where population growth and agricultural development are intensive.

Saline as a vehicle control does not alter ventilation in male CD-1 mice.

PubMed

Receno, Candace N; Glausen, Taylor G; DeRuisseau, Lara R

2018-05-01

Saline (0.9% NaCl) is used in clinical and research settings as a vehicle for intravenous drug administration. While saline is a standard control in mouse studies, there are reports of hyperchloremic metabolic acidosis in high doses. It remains unknown if metabolic acidosis occurs in mice and/or if compensatory increases in breathing frequency and tidal volume accompany saline administration. It was hypothesized that saline administration alters blood pH and the pattern of breathing in conscious CD-1 male mice exposed to air or hypoxia (10% O 2 , balanced N 2 ). Unrestrained barometric plethysmography was used to quantify breathing frequency (breaths/min; bpm), tidal volume (VT; mL/breath/10 g body weight (BW)), and minute ventilation (VE; mL/min/10 g BW) in two designs: (1) 11-week-old mice with no saline exposure (n = 11) compared to mice with 7 days of 0.9% saline administration (intraperitoneal, i.p.; 10 mL/kg body mass; n = 6). and (2) 17-week-old mice tested before (PRE) and after 1 day (POST1, n = 6) or 7 days (POST7, n = 5) of saline (i.p.; 10 mL/kg body mass). There were no differences when comparing frequency, VT, or VE between groups for either design with room air or hypoxia exposures. Hypoxia increased frequency, VT, and VE compared to room air. Moreover, conscious blood sampling showed no differences in pH, p a CO 2 , p aO2 , or HCO3- in mice without or with 7 days of saline. These findings reveal no differences in ventilation following 1 and/or 7 days of saline administration in mice. Therefore, the use of 0.9% saline as a control is supported for studies evaluating the control of breathing in mice. © 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

Investigation of effects of temperature, salinity, and electrode design on the performance of an electrochemical coliform detector

NASA Technical Reports Server (NTRS)

Grana, D. C.

1979-01-01

The results of two research programs to determine the optimum detector design for measuring fecal coliforms in saline waters for operational systems are presented. One program was concerned with the effects of temperature and salinity on endpoint response time, and the other, the interaction between electrode configurations and the test organisms. Test results show that the endpoint response time is related to salinity and seawater temperature; however, these results can be minimized by the correct choice of growth media. Electrode configurations were developed from stainless steel, Parlodion-coated stainless steel, and platinum that circumvented problems associated with the commercial redox electrodes.

Hemolymph chemistry and histopathological changes in Pacific oysters (Crassostrea gigas) in response to low salinity stress.

PubMed

Knowles, Graeme; Handlinger, Judith; Jones, Brian; Moltschaniwskyj, Natalie

2014-09-01

This study described seasonal differences in the histopathological and hemolymph chemistry changes in different family lines of Pacific oysters, Crassostrea gigas, in response to the stress of an abrupt change to low salinity, and mechanical grading. The most significant changes in pallial cavity salinity, hemolymph chemistry and histopathological findings occurred in summer at low salinity. In summer (water temperature 18°C) at low salinity, 9 (25.7% of full salinity), the mean pallial cavity salinity in oysters at day 3 was 19.8±1.6 (SE) and day 10 was 22.8±1.6 (SE) lower than oysters at salinity 35. Associated with this fall in pallial cavity salinity, mean hemolymph sodium for oysters at salinity 9 on day 3 and 10 were 297.2mmol/L±20(SE) and 350.4mmol/L±21.3(SE) lower than oysters at salinity 35. Similarly mean hemolymph potassium in oysters held at salinity 9 at day 3 and 10 were 5.6mmol/L±0.6(SE) and 7.9mmol/L±0.6 (SE) lower than oysters at salinity 35. These oysters at low salinity had expanded intercellular spaces and significant intracytoplasmic vacuolation distending the cytoplasm of epithelial cells in the alimentary tract and kidney and hemocyte infiltrate (diapedesis) within the alimentary tract wall. In contrast, in winter (water temperature 8°C) oyster mean pallial cavity salinity only fell at day 10 and this was by 6.0±0.6 (SE) compared to that of oysters at salinity 35. There were limited histopathological changes (expanded intercellular spaces and moderate intracytoplasmic vacuolation of renal epithelial cells) in these oysters at day 10 in low salinity. Mechanical grading and family line did not influence the oyster response to sudden low salinity. These findings provide additional information for interpretation of non-lethal, histopathological changes associated with temperature and salinity variation. Copyright © 2014 Elsevier Inc. All rights reserved.

Diatom Cell Size, Coloniality and Motility: Trade-Offs between Temperature, Salinity and Nutrient Supply with Climate Change

PubMed Central

Svensson, Filip; Norberg, Jon; Snoeijs, Pauline

2014-01-01

Reduction in body size has been proposed as a universal response of organisms, both to warming and to decreased salinity. However, it is still controversial if size reduction is caused by temperature or salinity on their own, or if other factors interfere as well. We used natural benthic diatom communities to explore how “body size” (cells and colonies) and motility change along temperature (2–26°C) and salinity (0.5–7.8) gradients in the brackish Baltic Sea. Fourth-corner analysis confirmed that small cell and colony sizes were associated with high temperature in summer. Average community cell volume decreased linearly with 2.2% per °C. However, cells were larger with artificial warming when nutrient concentrations were high in the cold season. Average community cell volume increased by 5.2% per °C of artificial warming from 0 to 8.5°C and simultaneously there was a selection for motility, which probably helped to optimize growth rates by trade-offs between nutrient supply and irradiation. Along the Baltic Sea salinity gradient cell size decreased with decreasing salinity, apparently mediated by nutrient stoichiometry. Altogether, our results suggest that climate change in this century may polarize seasonality by creating two new niches, with elevated temperature at high nutrient concentrations in the cold season (increasing cell size) and elevated temperature at low nutrient concentrations in the warm season (decreasing cell size). Higher temperature in summer and lower salinity by increased land-runoff are expected to decrease the average cell size of primary producers, which is likely to affect the transfer of energy to higher trophic levels. PMID:25279720

Coastal circulation and sediment dynamics along West Maui, Hawaii; PART IV: measurements of waves, currents, temperature, salinity and turbidity in Honolua Bay, Northwest Maui: 2003-2004

USGS Publications Warehouse

Storlazzi, Curt D.; Presto, M. Kathy

2005-01-01

High-resolution measurements of waves, currents, water levels, temperature, salinity and turbidity were made in Honolua Bay, northwest Maui, Hawaii, during 2003 and 2004 to better understand coastal dynamics in coral reef habitats. Measurements were acquired through two different collection methods. Two hydrographic survey cruises were conducted to acquire spatially-extensive, but temporally-limited, three-dimensional measurements of currents, temperature, salinity and turbidity in the winter and summer of 2003. From mid 2003 through early 2004, a bottom-mounted instrument package was deployed in a water depth of 10 m to collect long-term, single-point high-resolution measurements of waves, currents, water levels, temperature, salinity and turbidity. The purpose of these measurements was to collect hydrographic data to learn how waves, currents and water column properties such as water temperature, salinity and turbidity vary spatially and temporally in a near-shore coral reef system adjacent to a major stream drainage. These measurements support the ongoing process studies being conducted as part of the U.S. Geological Survey (USGS) Coastal and Marine Geology Program's Coral Reef Project; the ultimate goal is to better understand the transport mechanisms of sediment, larvae, pollutants and other particles in coral reef settings. This report, the final part in a series, describes data acquisition, processing and analysis. Previous reports provided data and results on: Long-term measurements of currents, temperature, salinity and turbidity off Kahana (PART I), the spatial structure of currents, temperature, salinity and suspended sediment along West Maui (PART II), and flow and coral larvae and sediment dynamics during the 2003 summer spawning season (PART III).

Diatom cell size, coloniality and motility: trade-offs between temperature, salinity and nutrient supply with climate change.

PubMed

Svensson, Filip; Norberg, Jon; Snoeijs, Pauline

2014-01-01

Reduction in body size has been proposed as a universal response of organisms, both to warming and to decreased salinity. However, it is still controversial if size reduction is caused by temperature or salinity on their own, or if other factors interfere as well. We used natural benthic diatom communities to explore how "body size" (cells and colonies) and motility change along temperature (2-26°C) and salinity (0.5-7.8) gradients in the brackish Baltic Sea. Fourth-corner analysis confirmed that small cell and colony sizes were associated with high temperature in summer. Average community cell volume decreased linearly with 2.2% per °C. However, cells were larger with artificial warming when nutrient concentrations were high in the cold season. Average community cell volume increased by 5.2% per °C of artificial warming from 0 to 8.5°C and simultaneously there was a selection for motility, which probably helped to optimize growth rates by trade-offs between nutrient supply and irradiation. Along the Baltic Sea salinity gradient cell size decreased with decreasing salinity, apparently mediated by nutrient stoichiometry. Altogether, our results suggest that climate change in this century may polarize seasonality by creating two new niches, with elevated temperature at high nutrient concentrations in the cold season (increasing cell size) and elevated temperature at low nutrient concentrations in the warm season (decreasing cell size). Higher temperature in summer and lower salinity by increased land-runoff are expected to decrease the average cell size of primary producers, which is likely to affect the transfer of energy to higher trophic levels.

Draft Genome Sequence of Bacillus urumqiensis BZ-SZ-XJ18T, a Moderately Haloalkaliphilic Bacterium Isolated from a Saline-Alkaline Lake.

PubMed

Liao, Ziya; Ren, Chao; Guo, Xiaomeng; Yan, Yanchun; Li, Jun; Zhao, Baisuo

2018-05-31

The moderately haloalkaliphilic bacterium Bacillus urumqiensis BZ-SZ-XJ18 T was isolated from a saline-alkaline lake located in the Xinjiang Uyghur Autonomous Region of China. Optimum growth occurred at the total Na + concentration of 1.08 M, with a broad optimum pH of 8.5 to 9.5. The draft genome consists of approximately 3.28 Mb and contains 3,228 predicted genes. A number of genes associated with adaptation strategies for osmotic balance and alkaline pH homeostasis were identified, providing pertinent insight into specific adaptations to the double-extreme environment. Copyright © 2018 Liao et al.

Modeling the morphogenesis of brine channels in sea ice.

PubMed

Kutschan, B; Morawetz, K; Gemming, S

2010-03-01

Brine channels are formed in sea ice under certain constraints and represent a habitat of different microorganisms. The complex system depends on a number of various quantities as salinity, density, pH value, or temperature. Each quantity governs the process of brine channel formation. There exists a strong link between bulk salinity and the presence of brine drainage channels in growing ice with respect to both the horizontal and vertical planes. We develop a suitable phenomenological model for the formation of brine channels both referring to the Ginzburg-Landau theory of phase transitions as well as to the chemical basis of morphogenesis according to Turing. It is possible to conclude from the critical wave number on the size of the structure and the critical parameters. The theoretically deduced transition rates have the same magnitude as the experimental values. The model creates channels of similar size as observed experimentally. An extension of the model toward channels with different sizes is possible. The microstructure of ice determines the albedo feedback and plays therefore an important role for large-scale global circulation models.

Extremophilic and extremotolerant actinomycetes in different soil types

NASA Astrophysics Data System (ADS)

Zenova, G. M.; Manucharova, N. A.; Zvyagintsev, D. G.

2011-04-01

Problems on the resistance of soil actinomycetes to various environmental factors (pH, salinity, temperature, and moisture) are discussed. Actinomycetes as a special group of prokaryotes were revealed to have a greater range of tolerance to these factors than was thought earlier. The regularities of the distribution of extremophilic and extremotolerant actinomycetes developing in unusual for mycelial bacteria conditions, their structural-functional characteristics, and their taxonomic composition were determined. The predominance of acidophilic representatives of the Micromonospora genus in acid soils (typical peat, soddy-podzolic, and taiga podzol) and the haloalkaliphilic Streptomyces pluricilirescens and S. prunicolor species in desert saline soils are shown. The specific features of the actinomycete complexes on thermal fields of the weakly developed stratified volcanic soils are described. In these complexes, the thermophilic forms were represented only by species of the Micromonospora genus; and the mesophilic forms, by Microbispora species. In the periodically heated desert soils, among the thermophilic actinomycetes, representatives of rare Actinomadura, Saccharopolyspora and Streptosporangium genera along with Streptomyces species were indicated. The mechanisms of the resistance of the actinomycetes to the extreme environmental conditions are discussed.

Interactive effects of water temperature and salinity on growth and mortality of eastern oysters, Crassostrea virginica: A meta-analysis using 40 years of monitoring data

USGS Publications Warehouse

Lowe, Michael R.; Sehlinger, Troy; Soniat, Thomas M.; LaPeyre, Megan K.

2017-01-01

Despite nearly a century of exploitation and scientific study, predicting growth and mortality rates of the eastern oyster (Crassostrea virginica) as a means to inform local harvest and management activities remains difficult. Ensuring that models reflect local population responses to varying salinity and temperature combinations requires locally appropriate models. Using long-term (1988 to 2015) monitoring data from Louisiana's public oyster reefs, we develop regionally specific models of temperature- and salinity-driven mortality (sack oysters only) and growth for spat (<25 mm), seed (25–75 mm), and sack (>75 mm) oyster size classes. The results demonstrate that the optimal combination of temperature and salinity where Louisiana oysters experience reduced mortality and fast growth rates is skewed toward lower salinities and higher water temperatures than previous models have suggested. Outside of that optimal range, oysters are commonly exposed to combinations of temperature and salinity that are correlated with high mortality and reduced growth. How these combinations affect growth, and to a lesser degree mortality, appears to be size class dependent. Given current climate predictions for the region and ongoing large-scale restoration activities in coastal Louisiana, the growth and mortality models are a critical step toward ensuring sustainable oyster reefs for long-term harvest and continued delivery of the ecological services in a changing environment.

Change in coccolith size and morphology due to response to temperature and salinity in coccolithophore Emiliania huxleyi (Haptophyta) isolated from the Bering and Chukchi seas

NASA Astrophysics Data System (ADS)

Saruwatari, Kazuko; Satoh, Manami; Harada, Naomi; Suzuki, Iwane; Shiraiwa, Yoshihiro

2016-05-01

Strains of the coccolithophore Emiliania huxleyi (Haptophyta) collected from the subarctic North Pacific and Arctic oceans in 2010 were established as clone cultures and have been maintained in the laboratory at 15 °C and 32 ‰ salinity. To study the physiological responses of coccolith formation to changes in temperature and salinity, growth experiments and morphometric investigations were performed on two strains, namely MR57N isolated from the northern Bering Sea and MR70N at the Chukchi Sea. This is the first report of a detailed morphometric and morphological investigation of Arctic Ocean coccolithophore strains. The specific growth rates at the logarithmic growth phases in both strains markedly increased as temperature was elevated from 5 to 20 °C, although coccolith productivity (estimated as the percentage of calcified cells) was similar at 10-20 % at all temperatures. On the other hand, the specific growth rate of MR70N was affected less by changes in salinity in the range 26-35 ‰, but the proportion of calcified cells decreased at high and low salinities. According to scanning electron microscopy (SEM) observations, coccolith morphotypes can be categorized into Type B/C on the basis of their biometrical parameters. The central area elements of coccoliths varied from thin lath type to well-calcified lath type when temperature was increased or salinity was decreased, and coccolith size decreased simultaneously. Coccolithophore cell size also decreased with increasing temperature, although the variation in cell size was slightly greater at the lower salinity level. This indicates that subarctic and arctic coccolithophore strains can survive in a wide range of seawater temperatures and at lower salinities with change in their morphology. Because all coccolith biometric parameters followed the scaling law, the decrease in coccolith size was caused simply by the reduced calcification. Taken together, our results suggest that calcification productivity may be used to predict future oceanic environmental conditions in the polar regions.

Prevention of Oral Candidiasis After Free Flap Surgery: Role of 3% Sodium Bicarbonate Saline in Oral Care.

PubMed

Yang, Yue; Zhang, Fang; Lyu, Xin; Yan, Zhimin; Hua, Hong; Peng, Xin

2017-03-01

Relevant reports about oral candidiasis status and prevention measures after free flap surgery for the oral and maxillofacial region are limited. The present study explored oral candidiasis status after free flap surgery and its prevention through a prospective comparative study. One hundred four patients were randomized to a control group (n = 54) and an experimental group (n = 50). Compared with the control group, the experimental group was provided an additional 3% sodium bicarbonate saline solution for oral care after free flap surgery. The incidence of oral candidiasis was evaluated by objective examination (saliva culture and salivary pH measurement) and subjective evaluation (clinical signs of oral candidiasis) at admission and from postoperative days 1 to 14. The salivary pH values of the 2 groups were lower than the normal salivary pH, and postoperative salivary pH values were always lower than the active range of oral lysozymes in the control group. The salivary pH values of the experimental group were higher than those of the control group from postoperative days 6 to 14 (P < .05). The incidence of oral candidiasis was 13.0% in the control group, which was higher than that in the experimental group (2.0%; P < .05). In addition, advanced age, use of a free flap for the simultaneous repair of intraoral and paraoral defects, and a combination of 2 antibiotic types were risk factors for oral candidiasis. Oral candidiasis was common in patients after free flap reconstruction surgery, and the use of 3% sodium bicarbonate saline solution for oral care effectively prevented it. Copyright © 2016 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

Salinity bias on the foraminifera Mg/Ca thermometry: Correction procedure and implications for past ocean hydrographic reconstructions

NASA Astrophysics Data System (ADS)

Mathien-Blard, Elise; Bassinot, Franck

2009-12-01

Mg/Ca in foraminiferal calcite has recently been extensively used to estimate past oceanic temperatures. Here we show, however, that the Mg/Ca temperature relationship of the planktonic species Globigerinoides ruber is significantly affected by seawater salinity, with a +1 psu change in salinity resulting in a +1.6°C bias in Mg/Ca temperature calculations. If not accounted for, such a bias could lead, for instance, to systematic overestimations of Mg/Ca temperatures during glacial periods, when global ocean salinity had significantly increased compared to today. We present here a correction procedure to derive unbiased sea surface temperatures (SST) and δ18Osw from G. ruber TMg/Ca and δ18Of measurements. This correction procedure was applied to a sedimentary record to reconstruct hydrographic changes since the Last Glacial Maximum (LGM) in the Western Pacific Warm Pool. While uncorrected TMg/Ca data indicate a 3°C warming of the Western Pacific Warm Pool since the LGM, the salinity-corrected SST result in a stronger warming of 4°C.

Results from a lab study of melting sea ice

NASA Astrophysics Data System (ADS)

Wiese, M.; Griewank, P.; Notz, D.

2012-04-01

Sea-ice melting is a complex process which is not fully understood yet. In order to study sea-ice melt in detail we perform lab experiments in an approximately 2x0.7x1.2 m large tank in a cold room. We grow sea ice with different salinities at least 10 cm thick. Then we let the ice melt at different air temperatures and oceanic heat fluxes. During the melt period, we measure the evolution of ice thickness, internal temperature, salinity and surface temperature. We will present results from roughly five months of experiments. Topics will include the influence of bulk salinity on melt rates and the surface temperature. The effects of flushing on the salinity evolution and detailed thermal profiles will also be included. To investigate these processes we focus on the energy budget and the salinity evolution. These topics are linked since the thermodynamic properties of sea ice (heat capacity, heat conductivity and latent heat of fusion) are very sensitive to salinity variations. For example the heat capacity of sea ice increases greatly as the temperature approaches the melting point. This increase results in non-linear temperature profiles and enhances heat conduction into the ice. The salinity evolution during the growth phase has been investigated and measured in multiple studies over the last decades. In contrast there are no detailed lab measurements of melting ice available to quantify the effects of flushing melt water and ponding. This is partially due to the fact that the heterogeneity of melting sea ice makes it much more difficult to measure representative values.

Comparative sensitivity of Crassostrea angulata and Crassostrea gigas embryo-larval development to As under varying salinity and temperature.

PubMed

Moreira, Anthony; Figueira, Etelvina; Libralato, Giovanni; Soares, Amadeu M V M; Guida, Marco; Freitas, Rosa

2018-06-07

Oysters are a diverse group of marine bivalves that inhabit coastal systems of the world's oceans, providing a variety of ecosystem services, and represent a major socioeconomic resource. However, oyster reefs have become inevitably impacted from habitat destruction, overfishing, pollution and disease outbreaks that have pushed these structures to the break of extinction. In addition, the increased frequency of climate change related events promise to further challenge oyster species survival worldwide. Oysters' early embryonic development is likely the most vulnerable stage to climate change related stressors (e.g. salinity and temperature shifts) as well as to pollutants (e.g. arsenic), and therefore can represent the most important bottleneck that define populations' survival in a changing environment. In light of this, the present study aimed to assess two important oyster species, Crassostrea angulata and Crassostrea gigas embryo-larval development, under combinations of salinity (20, 26 and 33), temperature (20, 24 and 28 °C) and arsenic (As) exposure (0, 30, 60, 120, 240, 480, 960 and 1920 μg. As L -1 ), to infer on different oyster species capacity to cope with these environmental stressors under the eminent threat of climate change and increase of pollution worldwide. Results showed differences in each species range of salinity and temperature for successful embryonic development. For C angulata, embryo-larval development was successful at a narrower range of both salinity and temperature, compared to C. gigas. Overall, As induced higher toxicity to C. angulata embryos, with calculated EC50 values at least an order of magnitude lower than those calculated for C. gigas. The toxicity of As (measured as median effective concentration, EC50) showed to be influenced by both salinity and temperature in both species. Nonetheless, salinity had a greater influence on embryos' sensitivity to As. This pattern was mostly noticed for C. gigas, with lower salinity inducing higher sensitivity to As. Results were discussed considering the existing literature and suggest that C. angulata populations are likely to become more vulnerable under near future predictions for temperature rise, salinity shifts and pollution. Copyright © 2018 Elsevier Ltd. All rights reserved.

Species Profiles. Life Histories and Environmental Requirements of Coastal Fishes and Invertebrates (Mid-Atlantic). Blue Crab

DTIC Science & Technology

1989-03-01

size only by Music 1979). molting (Hay 1905). Zoeal development depends on salinity and temperature, Growth and maturation proceed but development time...substrates. the effects depends on the toxicant, concentration, time exposed, salinity , tidal cycle, age and molt phase of Other Environmental Factors...Temperature .......................................................... 11 Salinity ............................................................. I11

Literature Review and Database of Relations Between Salinity and Aquatic Biota: Applications to Bowdoin National Wildlife Refuge, Montana

USGS Publications Warehouse

Gleason, Robert A.; Tangen, Brian A.; Laubhan, Murray K.; Finocchiaro, Raymond G.; Stamm, John F.

2009-01-01

Long-term accumulation of salts in wetlands at Bowdoin National Wildlife Refuge (NWR), Mont., has raised concern among wetland managers that increasing salinity may threaten plant and invertebrate communities that provide important habitat and food resources for migratory waterfowl. Currently, the U.S. Fish and Wildlife Service (USFWS) is evaluating various water management strategies to help maintain suitable ranges of salinity to sustain plant and invertebrate resources of importance to wildlife. To support this evaluation, the USFWS requested that the U.S. Geological Survey (USGS) provide information on salinity ranges of water and soil for common plants and invertebrates on Bowdoin NWR lands. To address this need, we conducted a search of the literature on occurrences of plants and invertebrates in relation to salinity and pH of the water and soil. The compiled literature was used to (1) provide a general overview of salinity concepts, (2) document published tolerances and adaptations of biota to salinity, (3) develop databases that the USFWS can use to summarize the range of reported salinity values associated with plant and invertebrate taxa, and (4) perform database summaries that describe reported salinity ranges associated with plants and invertebrates at Bowdoin NWR. The purpose of this report is to synthesize information to facilitate a better understanding of the ecological relations between salinity and flora and fauna when developing wetland management strategies. A primary focus of this report is to provide information to help evaluate and address salinity issues at Bowdoin NWR; however, the accompanying databases, as well as concepts and information discussed, are applicable to other areas or refuges. The accompanying databases include salinity values reported for 411 plant taxa and 330 invertebrate taxa. The databases are available in Microsoft Excel version 2007 (http://pubs.usgs.gov/sir/2009/5098/downloads/databases_21april2009.xls) and contain 27 data fields that include variables such as taxonomic identification, values for salinity and pH, wetland classification, location of study, and source of data. The databases are not exhaustive of the literature and are biased toward wetland habitats located in the glaciated North-Central United States; however, the databases do encompass a diversity of biota commonly found in brackish and freshwater inland wetland habitats.

Salinity and temperature variations reflecting on cellular PCNA, IGF-I and II expressions, body growth and muscle cellularity of a freshwater fish larvae.

PubMed

Martins, Y S; Melo, R M C; Campos-Junior, P H A; Santos, J C E; Luz, R K; Rizzo, E; Bazzoli, N

2014-06-01

The present study assessed the influence of salinity and temperature on body growth and on muscle cellularity of Lophiosilurus alexaxdri vitelinic larvae. Slightly salted environments negatively influenced body growth of freshwater fish larvae and we observed that those conditions notably act as an environmental influencer on muscle growth and on local expression of hypertrophia and hypeplasia markers (IGFs and PCNA). Furthermore, we could see that salinity tolerance for NaCl 4gl(-)(1) diminishes with increasing temperature, evidenced by variation in body and muscle growth, and by irregular morphology of the lateral skeletal muscle of larvae. We saw that an increase of both PCNA and autocrine IGF-II are correlated to an increase in fibre numbers and fibre diameter as the temperature increases and salinity diminishes. On the other hand, autocrine IGF-I follows the opposite way to the other biological parameters assessed, increasing as salinity increases and temperature diminishes, showing that this protein did not participate in muscle cellularity, but participating in molecular/cellular repair. Therefore, slightly salted environments may provide adverse conditions that cause some obstacles to somatic growth of this species, suggesting some osmotic expenditure with a salinity increment. Copyright © 2014 Elsevier Inc. All rights reserved.

Impact of Satellite Remote Sensing Data on Simulations of ...

EPA Pesticide Factsheets

We estimated surface salinity flux and solar penetration from satellite data, and performed model simulations to examine the impact of including the satellite estimates on temperature, salinity, and dissolved oxygen distributions on the Louisiana continental shelf (LCS) near the annual hypoxic zone. Rainfall data from the Tropical Rainfall Measurement Mission (TRMM) were used for the salinity flux, and the diffuse attenuation coefficient (Kd) from Moderate Resolution Imaging Spectroradiometer (MODIS) were used for solar penetration. Improvements in the model results in comparison with in situ observations occurred when the two types of satellite data were included. Without inclusion of the satellite-derived surface salinity flux, realistic monthly variability in the model salinity fields was observed, but important inter-annual variability wasmissed. Without inclusion of the satellite-derived light attenuation, model bottom water temperatures were too high nearshore due to excessive penetration of solar irradiance. In general, these salinity and temperature errors led to model stratification that was too weak, and the model failed to capture observed spatial and temporal variability in water-column vertical stratification. Inclusion of the satellite data improved temperature and salinity predictions and the vertical stratification was strengthened, which improved prediction of bottom-water dissolved oxygen. The model-predicted area of bottom-water hypoxia on the

Effects of Hydrogen-Rich Saline on Hepatectomy-Induced Postoperative Cognitive Dysfunction in Old Mice.

PubMed

Tian, Yue; Guo, Shanbin; Zhang, Yan; Xu, Ying; Zhao, Ping; Zhao, Xiaochun

2017-05-01

This study aims to investigate the protective effects and underlying mechanisms of hydrogen-rich saline on the cognitive functions of elder mice with partial hepatectomy-induced postoperative cognitive dysfunction (POCD). Ninety-six old male Kunming mice were randomly divided into 4 groups (n = 24 each): control group (group C), hydrogen-rich saline group (group H), POCD group (group P), and POCD + hydrogen-rich saline group (group PH). Cognitive function was subsequently assessed using Morris water-maze (MWM) test. TNF-α and IL-1β levels were measured by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, along with NF-κB activity determined by ELISA. The morphology of hippocampal tissues were further observed by HE staining. Learning and memory abilities of mice were significantly impaired at day 10 and day 14 post-surgery, as partial hepatectomy significantly prolonged the escape latency, decreased time at the original platform quadrant and frequency of crossing in group P when compared to group C (p < 0.05). The surgery also increased the contents of TNF-α, IL-1β, and NF-κB activity at all time points after surgery (p < 0.05). The introduction of hydrogen-rich saline (group PH) partially rescued spatial memory and learning as it shortened escape latency and increased time and crossing frequency of original platform compared to group P (p < 0.05). Moreover, such treatment also decreased TNF-α and IL-1β levels and NF-κB activity (p < 0.05). In addition, cell necrosis in the hippocampus induced by hepatectomy was also rescued by hydrogen-rich saline. Hydrogen-rich saline can alleviate POCD via inhibiting NF-κB activity in the hippocampus and reducing inflammatory response.

Influence of Soil Solution Salinity on Molybdenum Adsorption by Soils

USDA-ARS?s Scientific Manuscript database

Molybdenum (Mo) adsorption on five arid-zone soils from California was investigated as a function of equilibrium solution Mo concentration (0-30 mg L-1), solution pH (4-8), and electrical conductivity (EC = 0.3 or 8 dS m-1). Molybdenum adsorption decreased with increasing pH. An adsorption maximum...

Potential of the Moringa oleifera saline extract for the treatment of dairy wastewater: application of the response surface methodology.

PubMed

Formentini-Schmitt, Dalila Maria; Fagundes-Klen, Márcia Regina; Veit, Márcia Teresinha; Palácio, Soraya Moreno; Trigueros, Daniela Estelita Goes; Bergamasco, Rosangela; Mateus, Gustavo Affonso Pisano

2018-03-02

In this work, the coagulation/flocculation/sedimentation treatment of dairy wastewater samples was investigated through serial factorial designs utilizing the saline extract obtained from Moringa oleifera (Moringa) as a coagulant. The sedimentation time (ST), pH, Moringa coagulant (MC) dose and concentration of CaCl 2 have been evaluated through the response surface methodology in order to obtain the ideal turbidity removal (TR) conditions. The empirical quadratic model, in conjunction with the desirability function, demonstrated that it is possible to obtain TRs of 98.35% using a coagulant dose, concentration of CaCl 2 and pH of 280 mg L -1 , 0.8 mol L -1 and 9, respectively. The saline extract from Moringa presented its best efficiency at an alkaline pH, which influenced the reduction of the ST to a value of 25 min. It was verified that the increase in the solubility of the proteins in the Moringa stimulated the reduction of the coagulant content in the reaction medium, and it is related to the use of calcium chloride as an extracting agent of these proteins. The MC proved to be an excellent alternative for the dairy wastewater treatment, compared to the traditional coagulants.

Capillary Electrophoresis Analysis of Organic Amines and Amino Acids in Saline and Acidic Samples Using the Mars Organic Analyzer

NASA Astrophysics Data System (ADS)

Stockton, Amanda M.; Chiesl, Thomas N.; Lowenstein, Tim K.; Amashukeli, Xenia; Grunthaner, Frank; Mathies, Richard A.

2009-11-01

The Mars Organic Analyzer (MOA) has enabled the sensitive detection of amino acid and amine biomarkers in laboratory standards and in a variety of field sample tests. However, the MOA is challenged when samples are extremely acidic and saline or contain polyvalent cations. Here, we have optimized the MOA analysis, sample labeling, and sample dilution buffers to handle such challenging samples more robustly. Higher ionic strength buffer systems with pKa values near pH 9 were developed to provide better buffering capacity and salt tolerance. The addition of ethylaminediaminetetraacetic acid (EDTA) ameliorates the negative effects of multivalent cations. The optimized protocol utilizes a 75 mM borate buffer (pH 9.5) for Pacific Blue labeling of amines and amino acids. After labeling, 50 mM (final concentration) EDTA is added to samples containing divalent cations to ameliorate their effects. This optimized protocol was used to successfully analyze amino acids in a saturated brine sample from Saline Valley, California, and a subcritical water extract of a highly acidic sample from the Río Tinto, Spain. This work expands the analytical capabilities of the MOA and increases its sensitivity and robustness for samples from extraterrestrial environments that may exhibit pH and salt extremes as well as metal ions.

Capillary electrophoresis analysis of organic amines and amino acids in saline and acidic samples using the Mars organic analyzer.

PubMed

Stockton, Amanda M; Chiesl, Thomas N; Lowenstein, Tim K; Amashukeli, Xenia; Grunthaner, Frank; Mathies, Richard A

2009-11-01

The Mars Organic Analyzer (MOA) has enabled the sensitive detection of amino acid and amine biomarkers in laboratory standards and in a variety of field sample tests. However, the MOA is challenged when samples are extremely acidic and saline or contain polyvalent cations. Here, we have optimized the MOA analysis, sample labeling, and sample dilution buffers to handle such challenging samples more robustly. Higher ionic strength buffer systems with pK(a) values near pH 9 were developed to provide better buffering capacity and salt tolerance. The addition of ethylaminediaminetetraacetic acid (EDTA) ameliorates the negative effects of multivalent cations. The optimized protocol utilizes a 75 mM borate buffer (pH 9.5) for Pacific Blue labeling of amines and amino acids. After labeling, 50 mM (final concentration) EDTA is added to samples containing divalent cations to ameliorate their effects. This optimized protocol was used to successfully analyze amino acids in a saturated brine sample from Saline Valley, California, and a subcritical water extract of a highly acidic sample from the Río Tinto, Spain. This work expands the analytical capabilities of the MOA and increases its sensitivity and robustness for samples from extraterrestrial environments that may exhibit pH and salt extremes as well as metal ions.

Mg and Sr Incorporation in Foraminifer Shells: Patterns, Controls and Applications.

NASA Astrophysics Data System (ADS)

Lea, D. W.

2001-12-01

The incorporation of Mg and Sr in planktonic and benthic foraminifer shells is important for paleoceanographic research because of the potential to record physical and chemical changes in the oceanic environment. Pelagic shells are 99%+ CaCO3, and abundances of Mg and Sr are typically ~0.1%, requiring sensitive quantification methods such as ICP-MS or AES. Mg/Ca values range from 0.5 mmol/mol in cold planktics and benthics to ~5 mmol/mol in tropical planktics, with some species (Orbulina universa) having even higher values. The main control on Mg incorporation is temperature, but pH and salinity also exert small influences, presumably through calcification rate. The Mg/Ca content of the primary ontogenetic calcite can be altered by the addition of so-called gametogenic calcite, generally deposited in deep, colder waters. After deposition on the seafloor, dissolution becomes the main influence, with progressively lower Mg/Ca values in more dissolved samples. This loss appears to occur by preferential loss of the more Mg-rich portions of the shell, although the details remain unexplained. Sr/Ca values range from 0.9 in some benthic species (Uvigerina spp.) to 1.6 mmol/mol in some planktics. Culturing results suggest that temperature, salinity and pH all exert a weak control (i.e., 1% per ° C) on shell Sr, presumably through a kinetic effect. The main control appears to be related to environmental differences. For example, comparison of Sr/Ca in Neogloboquadrina pachyderma from plankton tows and cultures with core-top specimens indicates that the latter have significantly higher values, presumably due to deep crusting, perhaps added with a much higher calcification rate. This observation clearly demonstrates that Sr/Ca is not simply related to a single physical parameter such as temperature. Downcore records of shell Mg/Ca and Sr/Ca reveal substantial variability that can be correlated with known paleoceanographic change. For Mg/Ca, observed variations can largely be explained by climate-related variations in temperature. For Sr/Ca, it appears that observed variations related to secular changes in seawater Sr/Ca, but this cannot be fully substantiated without a more complete understanding of primary and post-depositional controls on shell composition.

Species Profiles. Life Histories and Environmental Requirements of Coastal Fishes and Invertebrates (Gulf of Mexico). COMMON RANGIA.

DTIC Science & Technology

1985-04-01

osmoconformer at salinities greater ENVIRONMENTAL REQUIREMENTS than 10 ppt, and an osmoregulator at lower salinities (Bedford and Anderson 1972a,b; Otto...1973, 1974) tested the combined effects of temperature (8 to 32°C) and salinity (0 to 20 ppt) on .. k6.. Temperature embryos and larvae of common...Bedford, W. B. , and J. W. Anderson. Allen, K. 1961. The effect of salin - 1972a. The physiological response ity on the amino acid concentra- of the

Kinetics of Ozone Inactivation of Infectious Prion Protein

PubMed Central

Ding, Ning; Price, Luke M.; Braithwaite, Shannon L.; Balachandran, Aru; Mitchell, Gordon; Belosevic, Miodrag

2013-01-01

The kinetics of ozone inactivation of infectious prion protein (PrPSc, scrapie 263K) was investigated in ozone-demand-free phosphate-buffered saline (PBS). Diluted infectious brain homogenates (IBH) (0.01%) were exposed to a predetermined ozone dose (10.8 ± 2.0 mg/liter) at three pHs (pH 4.4, 6.0, and 8.0) and two temperatures (4°C and 20°C). The inactivation of PrPSc was quantified by determining the in vitro destruction of PrPSc templating properties using the protein misfolding cyclic amplification (PMCA) assay and bioassay, which were shown to correlate well. The inactivation kinetics were characterized by both Chick-Watson (CW) and efficiency factor Hom (EFH) models. It was found that the EFH model fit the experimental data more appropriately. The efficacy of ozone inactivation of PrPSc was both pH and temperature dependent. Based on the EFH model, CT (disinfectant concentration multiplied by contact time) values were determined for 2-log10, 3-log10, and 4-log10 inactivation at the conditions under which they were achieved. Our results indicated that ozone is effective for prion inactivation in ozone-demand-free water and may be applied for the inactivation of infectious prion in prion-contaminated water and wastewater. PMID:23416994

The demosponge Halichondria (Halichondria) panicea (Pallas, 1766) as a novel source of biosurfactant-producing bacteria.

PubMed

Rizzo, Carmen; Syldatk, Christoph; Hausmann, Rudolf; Gerçe, Berna; Longo, Caterina; Papale, Maria; Conte, Antonella; De Domenico, Emilio; Michaud, Luigi; Lo Giudice, Angelina

2018-06-01

The Mediterranean sponge Halichondria (Halichondria) panicea was explored as a novel matrix for the isolation of biosurfactant-producing bacteria. A total of 38 (out of 56) isolates gave a good response to the employed screening tests (e.g., stable emulsion detection, surface tension measurement, hemolytic activity, and blue agar plate assay) and were selected for further analyses. The thin layer chromatography revealed a possible glucidic composition of biosurfactants. Most promising strains, i.e., those able to produce stable emulsion with percentage higher than 30% and yellow spots on TLC plates, were affiliated to the genera Pseudovibrio, Acinetobacter, and Bacillus. The biosurfactant production by two isolates (i.e., Acinetobacter sp. SpN134 and Pseudovibrio sp. SpE85) was evaluated under different culture conditions, in terms of temperature, NaCl concentration, and pH. Surface tension reduction ability was more stable than the emulsification, and resulted differently influenced by salinity, temperature, and pH. Acinetobacter sp. SpN134 resulted particularly efficient and competitive if compared with other well-known biosurfactant producers. Data suggest that sponges may represent a promising matrix for the isolation of biosurfactant-producing bacteria, reinforcing the growing interest towards filter-feeding organisms as underexplored sources of specialized bacteria. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Spectroscopic study of the microbial community in chemocline zones of relic meromictic lakes separating from the White Sea

NASA Astrophysics Data System (ADS)

Kharcheva, Anastasia V.; Krasnova, Elena D.; Voronov, Dmitry A.; Patsaeva, Svetlana V.

2015-03-01

As a result of a recent years study on the Karelia shore of the White Sea more than ten relict lakes in different stages of separation from the sea have been discovered. Five of them are located close to the Nikolai Pertsov White Sea Biological Station of Moscow State University. Such separated lakes are interesting to explore for their firm vertical stratification. Water layers differ not only by temperature, salinity and other physic and chemical characteristics and optical properties, but also by ibhabiting microorganisms and by the quality of dissolved organic matter. To study phototropic organisms in water sampled from different depths we used spectroscopic techniques. Identification of the main bands in the absorption and fluorescence spectra showed that there are two main groups of photosynthetic organisms in the redox zone (chemocline): unicellular algae containing chlorophyll a and green sulfur bacteria with bacteriochlorophylls c, d, e. Spectral data were compared with physical and chemical characteristics of the water layer (temperature, salinity, pH, dissolved oxygen and sunlight illumination at certain depth). It gave an opportunity to compare vertical profiles of oxygen and hydrogen sulphide concentration with the number and distribution of oxygenic and anoxygenic phototrophic microorganisms. Maximum abundance of both algae and green sulfur bacteria were achieved within the redox zone. Typical thickness of the layer with the highest concentration of microorganisms did not exceed 10-20 cm.

[Spatial-temporal distributions of dissolved inorganic carbon and its affecting factors in the Yellow River estuary].

PubMed

Guo, Xing-Sen; Lü, Ying-Chun; Sun, Zhi-Gao; Wang, Chuan-Yuan; Zhao, Quan-Sheng

2015-02-01

Estuary is an important area contributing to the global carbon cycle. In order to analyze the spatial-temporal distribution characteristics of the dissolved inorganic carbon (DIC) in the surface water of Yellow River estuary. Samples were collected in spring, summer, fall, winter of 2013, and discussed the correlation between the content of DIC and environmental factors. The results show that, the DIC concentration of the surface water in Yellow River estuary is in a range of 26.34-39.43 mg x L(-1), and the DIC concentration in freshwater side is higher than that in the sea side. In some areas where the salinity is less than 15 per thousand, the DIC concentration appears significant losses-the maximum loss is 20.46%. Seasonal distribution of performance in descending order is spring, fall, winter, summer. Through principal component analysis, it shows that water temperature, suspended solids, salinity and chlorophyll a are the main factors affecting the variation of the DIC concentration in surface water, their contribution rate is as high as 83% , and alkalinity, pH, dissolved organic carbon, dissolved oxygen and other factors can not be ignored. The loss of DIC in the low area is due to the calcium carbonate sedimentation. DIC presents a gradually increasing trend, which is mainly due to the effects of water retention time, temperature, outside input and environmental conditions.

Enhanced saturated fatty acids accumulation in cultures of newly-isolated strains of Schizochytrium sp. and Thraustochytriidae sp. for large-scale biodiesel production.

PubMed

Wang, Qiuzhen; Sen, Biswarup; Liu, Xianhua; He, Yaodong; Xie, Yunxuan; Wang, Guangyi

2018-08-01

Heterotrophic marine protists (Thraustochytrids) have received increasingly global attention as a renewable, sustainable and alternative source of biodiesel because of their high ability of saturated fatty acids (SFAs) accumulation. Yet, the influence of extrinsic factors (nutrients and environmental conditions) on thraustochytrid culture and optimal conditions for high SFAs production are poorly described. In the present study, two different thraustochytrid strains, Schizochytrium sp. PKU#Mn4 and Thraustochytriidae sp. PKU#Mn16 were studied for their growth and SFAs production profiles under various conditions (carbon, nitrogen, temperature, pH, KH 2 PO 4 , salinity, and agitation speed). Of the culture conditions, substrates (C and N) source and conc., temperature, and agitation speed significantly influenced the cell growth and SFAs production of both strains. Although both the strains were capable of growth and SFAs production in the broad range of culture conditions, their physiological responses to KH 2 PO 4 , pH, and salinity were dissimilar. Under their optimal batch culture conditions, peak SFAs productions of 3.3g/L and 2.2g/L with 62% and 49% SFAs contents (relative to total fatty acids) were achieved, respectively. The results of 5-L fed-batch fermentation under optimal conditions showed a nearly 4.5-fold increase in SFAs production (i.e., 7.5g/L) by both strains compared to unoptimized conditions. Of the two strains, the quality of biodiesel produced from the fatty acids of PKU#Mn4 met the biodiesel standard defined by ASTM6751. This study, to the knowledge of the authors, is the first comprehensive report of optimal fermentation conditions demonstrating enhanced SFAs production by strains belonging to two different thraustochytrid genera and provides the basis for large-scale biodiesel production. Copyright © 2018. Published by Elsevier B.V.

Benthic foraminifera record and geochemical studies to reconstruct the recent (~400 ya) paleoenvironment of Tomales Bay, California

NASA Astrophysics Data System (ADS)

Flores, S. C.; Hill, T. M.; Russell, A. D.; Brooks, G.

2010-12-01

We are conducting investigations of calcareous benthic foraminifera acquired from Tomales Bay, California to reconstruct geochemical conditions of the bay for the past ~400 years, a time period of both natural and anthropogenic environmental change. Tomales Bay, located ~50km northwest of San Francisco, is a long (20.4 km), narrow (0.7 - 1.7 km) and shallow (2.0 - 6.0 m) bay that exhibits long-residence times and is stratified in the summer due to seasonal hypersalinity. Tomales Bay is a unique environment for climate and environmental change research because of the wide documented variability in carbonate parameters (pH, alkalinity, DIC) due to freshwater input from terrestrial sources that decreases aragonite and calcite saturation states. The historical record provided by benthic foraminiferal species and geochemistry, sedimentary carbon (TOC and TIC) analyses, and investigations of recent (Rose-Bengal stained) foraminifera are being utilized to constrain 3 major processes: 1) the range of temperature and salinity shifts over the past 400 years, 2) the relative dominance of marine- vs. fresh-water sources to the bay, and 3) the extent to which freshwater input and runoff may influence water chemistry (saturation state, Ω) with impacts on foraminiferal calcification. Four sediment cores were acquired in 2009 and 2010, and subsequently age-dated utilizing radiocarbon analyses (seven samples). Results indicate an increase in preservation of agglutinated versus calcareous foraminiferal tests (shells) since the mid-1900’s, and greater abundances of agglutinated tests found near freshwater sources. The major calcareous foraminifera present in the record include Elphidium hannai, Elphidium excavatum, Ammonia tepida, and Buccella frigida. Results from oxygen and carbon stable isotope analyses as well as total organic carbon (by weight) for all the cores will also be presented. These results will be compared to modern observations and instrumental records of temperature, salinity and pH variability to understand the context of historical changes compared to modern shifts due to human influence.

Ocean acidification narrows the acute thermal and salinity tolerance of the Sydney rock oyster Saccostrea glomerata.

PubMed

Parker, Laura M; Scanes, Elliot; O'Connor, Wayne A; Coleman, Ross A; Byrne, Maria; Pörtner, Hans-O; Ross, Pauline M

2017-09-15

Coastal and estuarine environments are characterised by acute changes in temperature and salinity. Organisms living within these environments are adapted to withstand such changes, yet near-future ocean acidification (OA) may challenge their physiological capacity to respond. We tested the impact of CO 2 -induced OA on the acute thermal and salinity tolerance, energy metabolism and acid-base regulation capacity of the oyster Saccostrea glomerata. Adult S. glomerata were acclimated to three CO 2 levels (ambient 380μatm, moderate 856μatm, high 1500μatm) for 5weeks (24°C, salinity 34.6) before being exposed to a series of acute temperature (15-33°C) and salinity (34.2-20) treatments. Oysters acclimated to elevated CO 2 showed a significant metabolic depression and extracellular acidosis with acute exposure to elevated temperature and reduced salinity, especially at the highest CO 2 of 1500μatm. Our results suggest that the acute thermal and salinity tolerance of S. glomerata and thus its distribution will reduce as OA continues to worsen. Copyright © 2017 Elsevier Ltd. All rights reserved.

The effects of temperature and salinity on phosphate levels in two euryhaline crustacean species

NASA Astrophysics Data System (ADS)

Spaargaren, D. H.; Richard, P.; Ceccaldi, H. J.

Total phoshate, inorganic phosphate and organic (phospholipid) phosphate concentrations were determined in the blood of Carcinus maenas and in whole-animal homogenates of Penaeus japonicus acclimatized to various salinities and a high or a low temperature. In the blood of Carcinus, total and inorganic P concentrations range between 1.0 and 4.5 mmol · l -1; the amount of phospholipids is negligeable. The higher values were found at more extreme salinities. Low temperature is associated with low phosphate concentrations, particularly at intermediate salinities. Total P concentrations in Penaeus homogenates range between 10 and 60 mmol · 1 -1; phospholipid concentrations range between zero and 50 mmol · 1 -1. The higher values are again found at the extreme salinities. Inorganic P concentrations are almost constant — ca 10 mmol · 1 -1. No apparent effect of temperature on phosphate concentrations was observed. The results show clearly that osmotic stress influences severely the phosphate metabolism of the two species studied. Both species are able to accumulate phosphate at all experimental temperature/salinity combinations used, even when deprived of food. At extreme salinities, large quantities of phosphate are accumulated and converted to organic P compounds, most likely as phospholipids associated with the cell membranes. These effects of osmotic conditions in phosphate metabolism may offer an explanation for the effect of Ca ++ on membrane permeability as the regulation of both ions may be strongly interrelated, often under hormonal control.

Hydrogeological study of the aquifer system of the northern Sahara in the Algero-Tunisian border: A case study of Oued Souf region

NASA Astrophysics Data System (ADS)

Halassa, Younes; Zeddouri, Aziez; Mouhamadou, Ould Babasy; Kechiched, Rabah; Benhamida, Abdeldjebbar Slimane

2018-05-01

The aquifer system in The Algero-Tunisian border and Chotts region is mainly composed of two aquifers: The first is the Complex Terminal (CT) and the second is the Intercalary aquifer (CI). This study aims the identification and spatial evolution of factors that controlling the water quality in the Complex Terminal aquifer (CT) in the Chotts region (Oued Souf region - Southeastern of Algeria). The concentration of major elements, temperature, pH and salinity were monitored during 2015 in 34 wells from the CT aquifer. The geological, geophysical, hydrogeological and hydrochemical methods were applied in order to carried out a model for the investigated aquifer system and to characterize the hydrogeological and the geochemical behavior, as well as the geometrical and the lithological configuration. Multivariate statistical analyses such as Principal Component Analysis (PCA) were also used for the treatment of several data. Results show that the salinity follows the same regional distribution of Chloride, Sodium, Magnesium, Sulfate and Calcium. Note that the salinity shows low contents in the upstream part of investigated region suggesting restricted dissolution of salts. Hydro-chemical study and saturation indexes highlight the dominance of the dissolution and the precipitation of calcite, dolomite, anhydrite, gypsum and halite. The PCA analysis indicates that Na+, Cl-, Ca2+, Mg2+, SO42- and K+ variables that influence the water mineralization.

Bacterial Communities of Three Saline Meromictic Lakes in Central Asia

PubMed Central

Baatar, Bayanmunkh; Chiang, Pei-Wen; Rogozin, Denis Yu; Wu, Yu-Ting; Tseng, Ching-Hung; Yang, Cheng-Yu; Chiu, Hsiu-Hui; Oyuntsetseg, Bolormaa; Degermendzhy, Andrey G.; Tang, Sen-Lin

2016-01-01

Meromictic lakes located in landlocked steppes of central Asia (~2500 km inland) have unique geophysiochemical characteristics compared to other meromictic lakes. To characterize their bacteria and elucidate relationships between those bacteria and surrounding environments, water samples were collected from three saline meromictic lakes (Lakes Shira, Shunet and Oigon) in the border between Siberia and the West Mongolia, near the center of Asia. Based on in-depth tag pyrosequencing, bacterial communities were highly variable and dissimilar among lakes and between oxic and anoxic layers within individual lakes. Proteobacteria, Bacteroidetes, Cyanobacteria, Actinobacteria and Firmicutes were the most abundant phyla, whereas three genera of purple sulfur bacteria (a novel genus, Thiocapsa and Halochromatium) were predominant bacterial components in the anoxic layer of Lake Shira (~20.6% of relative abundance), Lake Shunet (~27.1%) and Lake Oigon (~9.25%), respectively. However, few known green sulfur bacteria were detected. Notably, 3.94% of all sequencing reads were classified into 19 candidate divisions, which was especially high (23.12%) in the anoxic layer of Lake Shunet. Furthermore, several hydro-parameters (temperature, pH, dissolved oxygen, H2S and salinity) were associated (P< 0.05) with variations in dominant bacterial groups. In conclusion, based on highly variable bacterial composition in water layers or lakes, we inferred that the meromictic ecosystem was characterized by high diversity and heterogenous niches. PMID:26934492

Bacterial Communities of Three Saline Meromictic Lakes in Central Asia.

PubMed

Baatar, Bayanmunkh; Chiang, Pei-Wen; Rogozin, Denis Yu; Wu, Yu-Ting; Tseng, Ching-Hung; Yang, Cheng-Yu; Chiu, Hsiu-Hui; Oyuntsetseg, Bolormaa; Degermendzhy, Andrey G; Tang, Sen-Lin

2016-01-01

Meromictic lakes located in landlocked steppes of central Asia (~2500 km inland) have unique geophysiochemical characteristics compared to other meromictic lakes. To characterize their bacteria and elucidate relationships between those bacteria and surrounding environments, water samples were collected from three saline meromictic lakes (Lakes Shira, Shunet and Oigon) in the border between Siberia and the West Mongolia, near the center of Asia. Based on in-depth tag pyrosequencing, bacterial communities were highly variable and dissimilar among lakes and between oxic and anoxic layers within individual lakes. Proteobacteria, Bacteroidetes, Cyanobacteria, Actinobacteria and Firmicutes were the most abundant phyla, whereas three genera of purple sulfur bacteria (a novel genus, Thiocapsa and Halochromatium) were predominant bacterial components in the anoxic layer of Lake Shira (~20.6% of relative abundance), Lake Shunet (~27.1%) and Lake Oigon (~9.25%), respectively. However, few known green sulfur bacteria were detected. Notably, 3.94% of all sequencing reads were classified into 19 candidate divisions, which was especially high (23.12%) in the anoxic layer of Lake Shunet. Furthermore, several hydro-parameters (temperature, pH, dissolved oxygen, H2S and salinity) were associated (P< 0.05) with variations in dominant bacterial groups. In conclusion, based on highly variable bacterial composition in water layers or lakes, we inferred that the meromictic ecosystem was characterized by high diversity and heterogenous niches.

Effects of temperature, salinity, and irradiance on the growth of harmful algal bloom species Phaeocystis globosa Scherffel (Prymnesiophyceae) isolated from the South China Sea

NASA Astrophysics Data System (ADS)

Xu, Ning; Huang, Bozhu; Hu, Zhangxi; Tang, Yingzhong; Duan, Shunshan; Zhang, Chengwu

2017-05-01

Blooms of Phaeocystis globosa have been frequently reported in Chinese coastal waters, causing serious damage to marine ecosystems. To better understand the ecological characteristics of P. globosa in Chinese coastal waters that facilitate its rapid expansion, the effects of temperature, salinity and irradiance on the growth of P. globosa from the South China Sea were examined in the laboratory. The saturating irradiance for the growth of P. globosa ( I s) was 60 μmol/(m2•s), which was lower than those of other harmful algal species (70-114 μmol/(m2•s)). A moderate growth rate of 0.22/d was observed at 2 μmol/(m2•s) (the minimum irradiance in the experiment), and photo-inhibition did not occur at 230 μmol/(m2•s) (the maximum irradiance in the experiment). Exposed to 42 different combinations of temperatures (10-31°C) and salinities (10-40) under saturating irradiance, P. globosa exhibited its maximum specific growth rate of 0.80/d at the combinations of 24°C and 35, and 27°C and 40. The optimum growth rates (>0.80/d) were observed at temperatures ranging from 24 to 27°C and salinities from 35 to 40. While P. globosa was able to grow well at temperatures from 20°C to 31°C and salinities from 20 to 40, it could not grow at temperatures lower than 15°C or salinities lower than 15. Factorial analysis revealed that temperature and salinity has similar influences on the growth of this species. This strain of P. globosa not only prefers higher temperatures and higher salinity, but also possesses a flexible nutrient competing strategy, adapted to lower irradiance. Therefore, the P. globosa population from South China Sea should belong to a new ecotype. There is also a potentially high risk of blooms developing in this area throughout the year.

Double-pass Mach-Zehnder fiber interferometer pH sensor.

PubMed

Tou, Zhi Qiang; Chan, Chi Chiu; Hong, Jesmond; Png, Shermaine; Eddie, Khay Ming Tan; Tan, Terence Aik Huang

2014-04-01

A biocompatible fiber-optic pH sensor based on a unique double-pass Mach-Zehnder interferometer is proposed. pH responsive poly(2-hydroxyethyl methacrylate-co-2-(dimethylamino)ethyl methacrylate) hydrogel coating on the fiber swells/deswells in response to local pH, leading to refractive index changes that manifest as shifting of interference dips in the optical spectrum. The pH sensor is tested in spiked phosphate buffer saline and demonstrates high sensitivity of 1.71  nm/pH, pH 0.004 limit of detection with good responsiveness, repeatability, and stability. The proposed sensor has been successfully applied in monitoring the media pH in cell culture experiments to investigate the relationship between pH and cancer cell growth.

Near-surface Salinity and Temperature Structure Observed with Dual-Sensor Drifters in the Subtropical South Pacific

NASA Astrophysics Data System (ADS)

Dong, Shenfu; Goni, Gustavo; Volkov, Denis; Lumpkin, Rick; Foltz, Gregory

2017-04-01

Three surface drifters equipped with temperature and salinity sensors at 0.2 m and 5 m depths were deployed in April/May 2015 in the subtropical South Pacific Ocean with the objective of measuring near-surface salinity differences seen by satellite and in situ sensors and examining the causes of the differences. Measurements from these drifters indicate that, on average, water at a depth of 0.2 m is about 0.013 psu fresher than at 5 m and about 0.024°C warmer. Events with large temperature and salinity differences between the two depths often occur when surface winds are weak. In addition to the expected surface freshening and cooling during rainfall events, surface salinification occurs under weak wind conditions when there is strong surface warming that enhances evaporation and upper ocean stratification. Further examination of the drifter measurements demonstrate that (i) the amount of surface freshening and vertical salinity gradient heavily depend on wind speed during rain events, (ii) salinity differences between 0.2 m and 5 m are positively correlated with the corresponding temperature differences, and (iii) temperature exhibits a diurnal cycle at both depths, whereas the diurnal cycle of salinity is observed only at 0.2 m when the wind speed is less than 4 m/s. Its phase is consistent with diurnal changes in surface temperature-induced evaporation. Below a wind speed of 6 m/s, the amplitudes of the diurnal cycles of temperature at both depths decrease with increasing wind speed. Wind speed also affects the phasing of the diurnal cycle of T5m with the time of maximum T5m increasing gradually with decreasing wind speed. Wind speed does not affect the phasing of the diurnal cycle of T0.2m. At 0.2 m and 5 m, the diurnal cycle of temperature also depends on surface solar radiation, with the amplitude and time of diurnal maximum increasing as solar radiation increases.

Multi-drug resistant toxigenic Vibrio cholerae O1 is persistent in water sources in New Bell-Douala, Cameroon

PubMed Central

2013-01-01

Background Cholera has been endemic in Douala, since 1971 when it was first recorded in Cameroon. Outbreaks have often started in slum areas of the city including New Bell. Despite the devastating nature of outbreaks, always resulting in high mortality and morbidity, a paucity of information exists on the reservoirs of the causative agent, V. cholerae, and factors maintaining its persistence. This has complicated disease prevention, resulting in frequent outbreaks of cholera. We investigated water sources in New Bell for contamination with V. cholerae O1 with pathogenic potential, to highlight their role in disease transmission. Antibiotic susceptibility pattern of isolates and the environmental factors maintaining its persistence were investigated. Method Water samples from various sources (taps, dug wells, streams) were analyzed for contamination with V. cholerae O1 using standard methods. Antibiotic susceptibility was determined by disc diffusion method. Pathogenic potential of isolates was determined by analyzing for genes for cholera toxin (ctx), toxin co-regulated pilus (tcpA), and zonula occludens toxin (zot) by PCR. Physico-chemical characteristics of water (pH, temperature and salinity) were investigated using standard methods. The Spearman’s Rank correlation was used to analyze the relationship between physico-chemical factors and the occurrence of V. cholerae O1. Differences were considered significant at P≤0.05. Results Twenty-five V. cholerae O1 strains were isolated from stream and well samples in both dry and rainy seasons. Twenty-three (92%) isolates were multidrug resistant. All isolates had genes for at least one virulence factor. Cholera toxin gene was detected in 7 isolates. Of the 15 isolates positive for tcpA gene, two had Classical type tcpA while 13 had tcpA El Tor. All tcpA Classical positive isolates were positive for ctx gene. Isolates were grouped into nine genotypes based on the genes analyzed. pH and salinity significantly correlated with isolation of V. cholerae O1. Conclusion Multidrug resistant Vibrio cholerae O1 with pathogenic potential is present in some wells and streams in study area. pH and salinity are among the factors maintaining the persistence of the organism. Findings indicate an urgent need for potable water supply in study area and in addition, regular disinfection of water from contaminated sources to prevent outbreak of cholera. PMID:23919373

Multi-drug resistant toxigenic Vibrio cholerae O1 is persistent in water sources in New Bell-Douala, Cameroon.

PubMed

Akoachere, Jane-Francis Tatah Kihla; Masalla, Thomas Njinuwoh; Njom, Henry Akum

2013-08-07

Cholera has been endemic in Douala, since 1971 when it was first recorded in Cameroon. Outbreaks have often started in slum areas of the city including New Bell. Despite the devastating nature of outbreaks, always resulting in high mortality and morbidity, a paucity of information exists on the reservoirs of the causative agent, V. cholerae, and factors maintaining its persistence. This has complicated disease prevention, resulting in frequent outbreaks of cholera. We investigated water sources in New Bell for contamination with V. cholerae O1 with pathogenic potential, to highlight their role in disease transmission. Antibiotic susceptibility pattern of isolates and the environmental factors maintaining its persistence were investigated. Water samples from various sources (taps, dug wells, streams) were analyzed for contamination with V. cholerae O1 using standard methods. Antibiotic susceptibility was determined by disc diffusion method. Pathogenic potential of isolates was determined by analyzing for genes for cholera toxin (ctx), toxin co-regulated pilus (tcpA), and zonula occludens toxin (zot) by PCR. Physico-chemical characteristics of water (pH, temperature and salinity) were investigated using standard methods. The Spearman's Rank correlation was used to analyze the relationship between physico-chemical factors and the occurrence of V. cholerae O1. Differences were considered significant at P≤0.05. Twenty-five V. cholerae O1 strains were isolated from stream and well samples in both dry and rainy seasons. Twenty-three (92%) isolates were multidrug resistant. All isolates had genes for at least one virulence factor. Cholera toxin gene was detected in 7 isolates. Of the 15 isolates positive for tcpA gene, two had Classical type tcpA while 13 had tcpA El Tor. All tcpA Classical positive isolates were positive for ctx gene. Isolates were grouped into nine genotypes based on the genes analyzed. pH and salinity significantly correlated with isolation of V. cholerae O1. Multidrug resistant Vibrio cholerae O1 with pathogenic potential is present in some wells and streams in study area. pH and salinity are among the factors maintaining the persistence of the organism. Findings indicate an urgent need for potable water supply in study area and in addition, regular disinfection of water from contaminated sources to prevent outbreak of cholera.

Inoculation of Brassica oxyrrhina with plant growth promoting bacteria for the improvement of heavy metal phytoremediation under drought conditions.

PubMed

Ma, Ying; Rajkumar, Mani; Zhang, Chang; Freitas, Helena

2016-12-15

The aim of this study was to investigate the effects of drought resistant serpentine rhizobacteria on plant growth and metal uptake by Brassica oxyrrhina under drought stress (DS) condition. Two drought resistant serpentine rhizobacterial strains namely Pseudomonas libanensis TR1 and Pseudomonas reactans Ph3R3 were selected based on their ability to stimulate seedling growth in roll towel assay. Further assessment on plant growth promoting (PGP) parameters revealed their ability to produce indole-3-acetic acid, siderophore and 1-aminocyclopropane-1-carboxylate deaminase. Moreover, both strains exhibited high resistance to various heavy metals, antibiotics, salinity and extreme temperature. Inoculation of TR1 and Ph3R3 significantly increased plant growth, leaf relative water and pigment content of B. oxyrrhina, whereas decreased concentrations of proline and malondialdehyde in leaves under metal stress in the absence and presence of DS. Regardless of soil water conditions, TR1 and Ph3R3 greatly improved organ metal concentrations, translocation and bioconcentration factors of Cu and Zn. The successful colonization and metabolic activities of P. libanensis TR1 and P. reactans Ph3R3 represented positive effects on plant development and metal phytoremediation under DS. These results indicate that these strains could be used as bio-inoculants for the improvement of phytoremediation of metal polluted soils under semiarid conditions. Copyright © 2016 Elsevier B.V. All rights reserved.

Chamber-Based Estimates of Methane Production in Coastal Estuarine Systems in Southern California

NASA Astrophysics Data System (ADS)

Brigham, B.; Lipson, D.; Lai, C.

2008-12-01

Wetland systems are believed to produce between 100 - 231 Tg CH4 yr-1 which is roughly 20% of global methane emissions. The uncertainty in methane emissions models stem from the lack of detailed information about methane gas production within regional wetland systems. The aim of this study is to report the range of methane fluxes observed along salinity gradients at two San Diego coastal wetland systems, the Tijuana Estuary (Tijuana River National Estuarine Research Reserve) and the Peñasquitos Lagoon (Torrey Pines State Park Reserve). Soil water samples are used to elucidate factors responsible for the observed variation in methane fluxes. Air samples were subsequently collected from the headspace of a static soil chamber and stored in pre- evacuated vials. Methane concentrations were analyzed within hours after collection by gas chromatography in the laboratory. The chemical and physical properties of the soil, including salinity, pH, redox potential and temperature are measured with a hand-held probe nearby soil collars. The biological properties of the soil, including dissolved organic carbon, nitrate, and ammonia levels are measured from soil water samples in the laboratory. We find that saline sites under direct tidal influence produced methane fluxes ranging from -3.10 to 9.10 (mean 2.18) mg CH4 m-2 day-1. We also find that brackish sites (0.6 to 3.2 ppt in salinity) with fresh water input from residential runoff at the Peñasquitos Lagoon produced methane fluxes ranging from 0.53 to 192.10 (mean 33.34) mg CH4 m-2 day-1. Sampling was done over the course of 5 weeks during August-September of 2008. We hypothesize that the contrasting methane fluxes found between the saline and the brackish sites is due primarily to the different salinity, and in turn sulfate levels found at the two sites. The reduction of sulfate to produce energy is more energetically favorable than the reduction of carbon dioxide to produce methane. Thus the presence of sulfate may act as a methanogensis inhibitor resulting in higher methane flux in low salinity conditions such as those found at the brackish sites.

Trematode infection modulates cockles biochemical response to climate change.

PubMed

Magalhães, Luísa; de Montaudouin, Xavier; Figueira, Etelvina; Freitas, Rosa

2018-10-01

Resulting mainly from atmospheric carbon dioxide (CO 2 ) build-up, seawater temperature rise is among the most important climate change related factors affecting costal marine ecosystems. Global warming will have implications on the water cycle, increasing the risk of heavy rainfalls and consequent freshwater input into the oceans but also increasing the frequency of extreme drought periods with consequent salinity increase. For Europe, by the end of the century, projections describe an increase of CO 2 concentration up to 1120 ppm (corresponding to 0.5 pH unit decrease), an increase in the water temperature up to 4 °C and a higher frequency of heavy precipitation. These changes are likely to impact many biotic interactions, including host-parasite relationships which are particularly dependent on abiotic conditions. In the present study, we tested the hypothesis that the edible cockle, Cerastoderma edule, exposed to different salinity, temperature and pH levels as proxy for climate change, modify the infection success of the trematode parasite Himasthla elongata, with consequences to cockles biochemical performance. The results showed that the cercariae infection success increased with acidification but higher biochemical alterations were observed in infected cockles exposed to all abiotic experimental stressful conditions tested. The present study suggested that changes forecasted by many models may promote the proliferation of the parasites infective stages in many ecosystems leading to enhanced transmission, especially on temperate regions, that will influence the geographical distribution of some diseases and, probably, the survival capacity of infected bivalves. Copyright © 2018 Elsevier B.V. All rights reserved.

Hypertonic Saline Suppresses NADPH Oxidase-Dependent Neutrophil Extracellular Trap Formation and Promotes Apoptosis.

PubMed

Nadesalingam, Ajantha; Chen, Jacky H K; Farahvash, Armin; Khan, Meraj A

2018-01-01

Tonicity of saline (NaCl) is important in regulating cellular functions and homeostasis. Hypertonic saline is administered to treat many inflammatory diseases, including cystic fibrosis. Excess neutrophil extracellular trap (NET) formation, or NETosis, is associated with many pathological conditions including chronic inflammation. Despite the known therapeutic benefits of hypertonic saline, its underlying mechanisms are not clearly understood. Therefore, we aimed to elucidate the effects of hypertonic saline in modulating NETosis. For this purpose, we purified human neutrophils and induced NETosis using agonists such as diacylglycerol mimetic phorbol myristate acetate (PMA), Gram-negative bacterial cell wall component lipopolysaccharide (LPS), calcium ionophores (A23187 and ionomycin from Streptomyces conglobatus ), and bacteria ( Pseudomonas aeruginosa and Staphylococcus aureus ). We then analyzed neutrophils and NETs using Sytox green assay, immunostaining of NET components and apoptosis markers, confocal microscopy, and pH sensing reagents. This study found that hypertonic NaCl suppresses nicotinamide adenine dinucleotide phosphate oxidase (NADPH2 or NOX2)-dependent NETosis induced by agonists PMA, Escherichia coli LPS (0111:B4 and O128:B12), and P. aeruginosa . Hypertonic saline also suppresses LPS- and PMA- induced reactive oxygen species production. It was determined that supplementing H 2 O 2 reverses the suppressive effect of hypertonic saline on NOX2-dependent NETosis. Many of the aforementioned suppressive effects were observed in the presence of equimolar concentrations of choline chloride and osmolytes (d-mannitol and d-sorbitol). This suggests that the mechanism by which hypertonic saline suppresses NOX2-dependent NETosis is via neutrophil dehydration. Hypertonic NaCl does not significantly alter the intracellular pH of neutrophils. We found that hypertonic NaCl induces apoptosis while suppressing NOX2-dependent NETosis. In contrast, hypertonic solutions do not suppress NOX2-independent NETosis. Although hypertonic saline partially suppresses ionomycin-induced NETosis, it enhances A23187-induced NETosis, and it does not alter S. aureus -induced NETosis. Overall, this study determined that hypertonic saline suppresses NOX2-dependent NETosis induced by several agonists; in contrast, it has variable effects on neutrophil death induced by NOX2-independent NETosis agonists. These findings are important in understanding the regulation of NETosis and apoptosis in neutrophils.

Assessing factors affecting the thermal properties of a passive thermal refuge using three-dimensional hydrodynamic flow and transport modeling

USGS Publications Warehouse

Decker, Jeremy D.; Swain, Eric D.; Stith, Bradley M.; Langtimm, Catherine A.

2013-01-01

Everglades restoration activities may cause changes to temperature and salinity stratification at the Port of the Islands (POI) marina, which could affect its suitability as a cold weather refuge for manatees. To better understand how the Picayune Strand Restoration Project (PSRP) may alter this important resource in Collier County in southwestern Florida, the USGS has developed a three-dimensional hydrodynamic model for the marina and canal system at POI. Empirical data suggest that manatees aggregate at the site during winter because of thermal inversions that provide warmer water near the bottom that appears to only occur in the presence of salinity stratification. To study these phenomena, the environmental fluid dynamics code simulator was used to represent temperature and salinity transport within POI. Boundary inputs were generated using a larger two-dimensional model constructed with the flow and transport in a linked overland-aquifer density-dependent system simulator. Model results for a representative winter period match observed trends in salinity and temperature fluctuations and produce temperature inversions similar to observed values. Modified boundary conditions, representing proposed PSRP alterations, were also tested to examine the possible effect on the salinity stratification and temperature inversion within POI. Results show that during some periods, salinity stratification is reduced resulting in a subsequent reduction in temperature inversion compared with the existing conditions simulation. This may have an effect on POI’s suitability as a passive thermal refuge for manatees and other temperature-sensitive species. Additional testing was completed to determine the important physical relationships affecting POI’s suitability as a refuge.

Warming and Inhibition of Salinization at the Ocean's Surface by Cyanobacteria

NASA Astrophysics Data System (ADS)

Wurl, O.; Bird, K.; Cunliffe, M.; Landing, W. M.; Miller, U.; Mustaffa, N. I. H.; Ribas-Ribas, M.; Witte, C.; Zappa, C. J.

2018-05-01

This paper describes high-resolution in situ observations of temperature and, for the first time, of salinity in the uppermost skin layer of the ocean, including the influence of large surface blooms of cyanobacteria on those skin properties. In the presence of the blooms, large anomalies of skin temperature and salinity of 0.95°C and -0.49 practical salinity unit were found, but a substantially cooler (-0.22°C) and saltier skin layer (0.19 practical salinity unit) was found in the absence of surface blooms. The results suggest that biologically controlled warming and inhibition of salinization of the ocean's surface occur. Less saline skin layers form during precipitation, but our observations also show that surface blooms of Trichodesmium sp. inhibit evaporation decreasing the salinity at the ocean's surface. This study has important implications in the assessment of precipitation over the ocean using remotely sensed salinity, but also for a better understanding of heat exchange and the hydrologic cycle on a regional scale.

Dynamics of harmful dinoflagellates driven by temperature and salinity in a northeastern Mediterranean lagoon.

PubMed

Dhib, Amel; Frossard, Victor; Turki, Souad; Aleya, Lotfi

2013-04-01

To attempt to determine the effects of temperature and salinity on the dynamics of the dinoflagellate community, a monthly sampling was carried out from October 2008 to March 2009 at eight sampling stations in Ghar El Melh Lagoon (GML; Mediterranean Sea, Northern Tunisia). Dinoflagellates were dominant among plankton, accounting for 73.9 % of the lagoon's overall plankton community, and were comprised of 25 different species among which 17 were reported in the literature as harmful. While no significant difference was found in the distribution of dinoflagellates among the stations, a strong monthly difference was observed. This temporal variability was due to an increase in the abundance of Prorocentrum micans from December to February, leading to a strong decrease in the Shannon diversity index from station to station. At the onset of P. micans development, dinoflagellate abundances reached 1.26.10(5) cells l(-1). A redundance analysis indicates that both temperature and salinity have a significant effect on the dynamics of the dinoflagellate community. Using a generalized additive model, both temperature and salinity appear to have significant nonlinear relationships with P. micans abundances. Model predictions indicate that outbreaks of P. micans may occur at a temperature below 22.5 °C and with salinity above 32.5. We discuss our results against a backdrop of climate change which, by affecting temperature and salinity, is likely to have an antagonistic impact on P. micans development and subsequently on the dinoflagellate dynamics in GML.

Quantification of Induced Hypothermia from Aseptic Scrub Applications during Rodent Surgery Preparation

PubMed Central

Skorupski, Anna M; Zhang, Jingyi; Ferguson, Danielle; Lawrence, Frank

2017-01-01

Laboratory mice (Mus musculus) are prone to develop hypothermia during anesthesia for surgery, thus potentially impeding anesthetic recovery, wound healing, and future health. The core body temperatures of isoflurane-anesthetized mice are influenced by the choice of supplemental heat sources; however, the contribution of various surgical scrubs on the body temperatures of mice under gas anesthesia has not been assessed. We sought to quantify the effect of using alcohol (70% isopropyl alcohol [IPA]) compared with saline to rinse away surgical scrub on the progression of hypothermia in anesthetized mice (n = 47). IPA, room-temperature saline, or warmed saline (37 °C) was combined with povidone–iodine and then assessed for effects on core (rectal) and surface (infrared) temperatures. Agents were applied to a 2×2-cm shaved abdominal area of mice maintained on a water-recirculating blanket (at 38 °C) under isoflurane anesthesia (1.5% to 2.0% at 0.6 L/min) for 30 min. Although all scrub regimens significantly decreased body temperature at the time of application, treatments that included povidone–iodine led to the coldest core temperatures, which persisted while mice were anesthetized. Compared with room-temperature saline and when combined with povidone–iodine, warming of saline did not ameliorate heat loss. IPA alone demonstrated the most dramatic cooling of both surface and core readings at application but generated an unanticipated warming (rebound) phase during which body temperatures equilibrated with those of controls within minutes of application. Although alcohol is inappropriate as a stand-alone agent for surgical skin preparation, IPA is a viable alternative to saline-based rinses in this context, and its use should be encouraged within institutional guidance for rodent surgical procedures without concern for prolonged hypothermia in mice. PMID:28903829

Distribution and significance of long-chain alkenones as salinity and temperature indicators in Spanish saline lake sediments

NASA Astrophysics Data System (ADS)

Pearson, Emma J.; Juggins, Steve; Farrimond, Paul

2008-08-01

We investigated relationships between sedimentary solvent-extractable long-chain alkenone (LCA) concentration and composition and environmental factors in a suite of endorheic lakes from inland Spain. LCAs were found in 14 of the 54 lakes examined, with concentrations comparable with those from previously published lacustrine settings. The composition of LCAs in our sites, however, contrast from the majority of those previously reported from lake environments; in our study the tri-unsaturated component is the most abundant component at most sites where LCAs are detected, and C 38:3 is the most abundant LCA in the majority of sites. LCA occurrence appears to be restricted to brackish-hypersaline sites and C 37 LCAs are absent above a salinity of ˜40 g L -1 suggesting a salinity control on LCA-producing organisms in these sites. Low concentrations of C 37 LCA components means U37k and U37k temperature indices are generally not applicable. Instead we find good relationships between C 38 components and (in particular mean autumn) temperature and the strongest LCA-temperature relationships are found when using a combination of all C 37 and C 38 compounds. We propose a new alkenone temperature index for lakes with elevated salinity and where the C 38 components dominate the LCA distributions. This is expressed as U3738k=0.0464×MAutAT-0.867 ( r2 = 0.80, n = 13). In this paper, we provide the first account of sedimentary LCA distributions from lakes in inland Spain, extending the range of environments within which these compounds have been found and highlighting their significance as indicators of both salinity and temperature in saline, endorheic lake environments. This has important implications for extending the potential role of LCAs as palaeoclimatic indicators in lacustrine environments.

Ontogenetic optimal temperature and salinity envelops of the copepod Eurytemora affinis in the Seine estuary (France)

NASA Astrophysics Data System (ADS)

Dur, Gaël; Souissi, Sami

2018-01-01

Temperature and salinity are important factors shaping the habitats of estuarine ectotherms. Their respective effect varies along the life history moments of species with a complex life cycle. Estuarine species, particularly those living in the salinity gradient, are concerned by habitat changes that can reduce their fitness. Consequently, efforts to define the importance of those two environmental variables on developmental stages are required to enable forecasting estuarine species' future distributions. The present study focuses on the main component of the Seine estuary's zooplankton, i.e. the calanoid copepod Eurytemora affinis, and aims: (i) to establish the role of temperature and salinity in designing the habitat of E. affinis within the Seine estuary; and (ii) to model the habitat of three groups of E. affinis defined through the life cycle as follows: all larval instars (N1-N6), the first to fourth juvenile instars (C1-C4), and the pre-adult and adults instars (C5-Adults). For this purpose, data from intensive field studies of zooplankton sampling during 2002-2010 were used. The fine-scale data, i.e., every 10-20 min, on density and abiotic conditions (salinity, temperature) provided inputs for the computation. We established regions in salinity-temperature space where the three groups of developmental instars exhibit higher densities. The computed habitats differ between developmental groups. In general, the preferendum of salinity increases with ontogeny. The optima of temperature are rather constant between developmental stages (∼14 °C). Our model can be used to determine E. affinis functional habitat (i.e., the spatial relation with structuring factors), to carry out retrospective analysis, and to test future distributions. The present study also emphasizes the need of data from appropriate sampling strategies to conduct habitat definition.

Soil salinity assessment through satellite thermography for different irrigated and rainfed crops

NASA Astrophysics Data System (ADS)

Ivushkin, Konstantin; Bartholomeus, Harm; Bregt, Arnold K.; Pulatov, Alim; Bui, Elisabeth N.; Wilford, John

2018-06-01

The use of canopy thermography is an innovative approach for salinity stress detection in plants. But its applicability for landscape scale studies using satellite sensors is still not well investigated. The aim of this research is to test the satellite thermography soil salinity assessment approach on a study area with different crops, grown both in irrigated and rainfed conditions, to evaluate whether the approach has general applicability. Four study areas in four different states of Australia were selected to give broad representation of different crops cultivated under irrigated and rainfed conditions. The soil salinity map was prepared by the staff of Geoscience Australia and CSIRO Land and Water and it is based on thorough soil sampling together with environmental modelling. Remote sensing data was captured by the Landsat 5 TM satellite. In the analysis we used vegetation indices and brightness temperature as an indicator for canopy temperature. Applying analysis of variance and time series we have investigated the applicability of satellite remote sensing of canopy temperature as an approach of soil salinity assessment for different crops grown under irrigated and rainfed conditions. We concluded that in all cases average canopy temperatures were significantly correlated with soil salinity of the area. This relation is valid for all investigated crops, grown both irrigated and rainfed. Nevertheless, crop type does influence the strength of the relations. In our case cotton shows only minor temperature difference compared to other vegetation classes. The strongest relations between canopy temperature and soil salinity were observed at the moment of a maximum green biomass of the crops which is thus considered to be the best time for application of the approach.

Effects of Climate Change on Temperature and Salinity in the Yaquina Estuary, Oregon (USA)

EPA Science Inventory

As part of a larger study to examine the effect of climate change (CC) on estuarine resources, we simulated the effect of rising sea level, alterations in river discharge, and increasing atmospheric temperatures on water properties (temperature and salinity) in the Yaquina Estuar...

Preparation, composition and functional properties of pennycress (Thlaspi arvense L.) seed protein isolates

USDA-ARS?s Scientific Manuscript database

This study evaluated two methods, saline extraction (SE) and conventional acid precipitation (AP), to recover proteins from pennycress (Thlaspi arvense L.) seed meal. SE was done using 0.1 M NaCl at 50ºC while AP involved alkaline extraction (pH 10) first followed by protein precipitation at pH 4. C...

Seasonal induced changes in spinach rhizosphere microbial community structure with varying salinity and drought.

PubMed

Mark Ibekwe, A; Ors, Selda; Ferreira, Jorge F S; Liu, Xuan; Suarez, Donald L

2017-02-01

Salinity is a common problem under irrigated agriculture, especially in low rainfall and high evaporative demand areas of southwestern United States and other semi-arid regions around the world. However, studies on salinity effects on soil microbial communities are relatively few while the effects of irrigation-induced salinity on soil chemical and physical properties and plant growth are well documented. In this study, we examined the effects of salinity, temperature, and temporal variability on soil and rhizosphere microbial communities in sand tanks irrigated with prepared solutions designed to simulate saline wastewater. Three sets of experiments with spinach (Spinacia oleracea L., cv. Racoon) were conducted under saline water during different time periods (early winter, late spring, and early summer). Bacterial 16S V4 rDNA region was amplified utilizing fusion primers designed against the surrounding conserved regions using MiSeq® Illumina sequencing platform. Across the two sample types, bacteria were relatively dominant among three phyla-the Proteobacteria, Cyanobacteria, and Bacteroidetes-accounted for 77.1% of taxa detected in the rhizosphere, while Proteobacteria, Bacteroidetes, and Actinobacteria accounted for 55.1% of taxa detected in soil. The results were analyzed using UniFrac coupled with principal coordinate analysis (PCoA) to compare diversity, abundance, community structure, and specific bacterial groups in soil and rhizosphere samples. Permutational analysis of variance (PERMANOVA) analysis showed that soil temperature (P=0.001), rhizosphere temperature (P=0.001), rhizosphere salinity (P=0.032), and evapotranspiration (P=0.002) significantly affected beta diversity of soil and rhizosphere microbial communities. Furthermore, salinity had marginal effects (P=0.078) on soil beta diversity. However, temporal variability differentially affected rhizosphere microbial communities irrigated with saline wastewater. Therefore, microbial communities in soils impacted by saline irrigation water respond differently to irrigation water quality and season of application due to temporal effects associated with temperature. Published by Elsevier B.V.

Production of consistent pain by intermittent infusion of sterile 5% hypertonic saline, followed by decrease of pain with cryotherapy.

PubMed

Long, Blaine C; Knight, Kenneth L; Hopkins, Ty; Parcell, Allen C; Feland, J Brent

2012-08-01

It is suggested that postinjury pain is difficult to examine; thus, investigators have developed experimental pain models. To minimize pain, cryotherapy (cryo) is applied, but reports on its effectiveness are limited. To investigate a pain model for the anterior knee and examine cryo in reducing the pain. Controlled laboratory study. Therapeutic modality laboratory. 30 physically active healthy male subjects who were free from any lower extremity orthopedic, neurological, cardiovascular, or endocrine pathologies. Perceived pain was measured every minute. Surface temperature was also assessed in the center of the patella and the popliteal fossa. There was a significant interaction between group and time (F68,864 = 3.0, P = .0001). At the first minute, there was no difference in pain between the 3 groups (saline/cryo = 4.80 ± 4.87 mm, saline/sham = 2.80 ± 3.55 mm, no saline/cryo = 4.00 ± 3.33 mm). During the first 5 min, pain increased from 4.80 ± 4.87 to 45.90 ± 21.17 mm in the saline/cryo group and from 2.80 ± 3.55 to 31.10 ± 20.25 mm in the saline/sham group. Pain did not change within the no-saline/cryo group, 4.00 ± 3.33 to 1.70 ± 1.70 mm. Pain for the saline/sham group remained constant for 17 min. Cryo decreased pain for 16 min in the saline/cryo group. There was no difference in preapplication surface temperature between or within each group. No change in temperature occurred within the saline/sham. Cooling and rewarming were similar in both cryo groups. Ambient temperature fluctuated less than 1°C during data collection. Intermittent infusion of sterile 5% hypertonic saline may be a useful experimental pain model in establishing a constant level of pain in a controlled laboratory setting. Cryotherapy decreased the induced anterior knee pain for 16 min.

On the calculation of air-sea fluxes of CO2 in the presence of temperature and salinity gradients

NASA Astrophysics Data System (ADS)

Woolf, D. K.; Land, P. E.; Shutler, J. D.; Goddijn-Murphy, L. M.; Donlon, C. J.

2016-02-01

The presence of vertical temperature and salinity gradients in the upper ocean and the occurrence of variations in temperature and salinity on time scales from hours to many years complicate the calculation of the flux of carbon dioxide (CO2) across the sea surface. Temperature and salinity affect the interfacial concentration of aqueous CO2 primarily through their effect on solubility with lesser effects related to saturated vapor pressure and the relationship between fugacity and partial pressure. The effects of temperature and salinity profiles in the water column and changes in the aqueous concentration act primarily through the partitioning of the carbonate system. Climatological calculations of flux require attention to variability in the upper ocean and to the limited validity of assuming "constant chemistry" in transforming measurements to climatological values. Contrary to some recent analysis, it is shown that the effect on CO2 fluxes of a cool skin on the sea surface is large and ubiquitous. An opposing effect on calculated fluxes is related to the occurrence of warm layers near the surface; this effect can be locally large but will usually coincide with periods of low exchange. A salty skin and salinity anomalies in the upper ocean also affect CO2 flux calculations, though these haline effects are generally weaker than the thermal effects.

High-density genetic map and identification of QTLs for responses to temperature and salinity stresses in the model brown alga Ectocarpus

PubMed Central

Avia, Komlan; Coelho, Susana M.; Montecinos, Gabriel J.; Cormier, Alexandre; Lerck, Fiona; Mauger, Stéphane; Faugeron, Sylvain; Valero, Myriam; Cock, J. Mark; Boudry, Pierre

2017-01-01

Deciphering the genetic architecture of adaptation of brown algae to environmental stresses such as temperature and salinity is of evolutionary as well as of practical interest. The filamentous brown alga Ectocarpus sp. is a model for the brown algae and its genome has been sequenced. As sessile organisms, brown algae need to be capable of resisting the various abiotic stressors that act in the intertidal zone (e.g. osmotic pressure, temperature, salinity, UV radiation) and previous studies have shown that an important proportion of the expressed genes is regulated in response to hyposaline, hypersaline or oxidative stress conditions. Using the double digest RAD sequencing method, we constructed a dense genetic map with 3,588 SNP markers and identified 39 QTLs for growth-related traits and their plasticity under different temperature and salinity conditions (tolerance to high temperature and low salinity). GO enrichment tests within QTL intervals highlighted membrane transport processes such as ion transporters. Our study represents a significant step towards deciphering the genetic basis of adaptation of Ectocarpus sp. to stress conditions and provides a substantial resource to the increasing list of tools generated for the species. PMID:28256542

Holocene oscillations in temperature and salinity of the surface subpolar North Atlantic.

PubMed

Thornalley, David J R; Elderfield, Harry; McCave, I Nick

2009-02-05

The Atlantic meridional overturning circulation (AMOC) transports warm salty surface waters to high latitudes, where they cool, sink and return southwards at depth. Through its attendant meridional heat transport, the AMOC helps maintain a warm northwestern European climate, and acts as a control on the global climate. Past climate fluctuations during the Holocene epoch ( approximately 11,700 years ago to the present) have been linked with changes in North Atlantic Ocean circulation. The behaviour of the surface flowing salty water that helped drive overturning during past climatic changes is, however, not well known. Here we investigate the temperature and salinity changes of a substantial surface inflow to a region of deep-water formation throughout the Holocene. We find that the inflow has undergone millennial-scale variations in temperature and salinity ( approximately 3.5 degrees C and approximately 1.5 practical salinity units, respectively) most probably controlled by subpolar gyre dynamics. The temperature and salinity variations correlate with previously reported periods of rapid climate change. The inflow becomes more saline during enhanced freshwater flux to the subpolar North Atlantic. Model studies predict a weakening of AMOC in response to enhanced Arctic freshwater fluxes, although the inflow can compensate on decadal timescales by becoming more saline. Our data suggest that such a negative feedback mechanism may have operated during past intervals of climate change.

Effect of Mg/Ca ratios on microbially induced carbonate precipitation

NASA Astrophysics Data System (ADS)

Balci, Nurgul; Demirel, Cansu; Seref Sonmez, M.; Kurt, M. Ali

2016-04-01

Influence of Mg/Ca ratios on microbially induced carbonate mineralogy were investigated by series of experiments carried out under various environmental conditions (Mg/Ca ratio, temperature and salinity). Halophilic bacterial cultures used for biomineralization experiments were isolated from hypersaline Lake Acıgöl (Denizli, SW Turkey), displaying extreme water chemistry with an average pH around 8.6 (Balci eta l.,2015). Enriched bacterial culture used in the experiments consisted of Halomonas saccharevitans strain AJ275, Halomonas alimentaria strain L7B; Idiomarina sp. TBZ29, 98% Idiomarina seosensis strain CL-SP19. Biomineralization experiments were set up using above enriched culture with Mg/Ca ratios of 0.05, 1, 4 and 15 and salinity of 8% and 15% experiments at 30oC and 10oC. Additionally, long-term biomineralization experiments were set up to last for a year, for Mg/Ca=4 and Mg/Ca=15 experiments at 30oC. For each experimental condition abiotic experiments were also conducted. Solution chemistry throughout incubation was monitored for Na, K, Mg, Ca, bicarbonate, carbonate, ammonium and phosphate for a month. At the end of the experiments, precipitates were collected and morphology and mineralogy of the biominerals were investigated and results were evaluated using the software DIFFRAC.SUITE EVA. Overall the preliminary results showed chemical precipitation of calcite, halite, hydromagnesite and sylvite. Results obtained from biological experiments indicate that, low Mg/Ca ratios (0.05 and 1) favor chlorapatite precipitation, whereas higher Mg/Ca ratios favor struvite precipitation. Biomineralization of dolomite, huntite and magnesite is favorable at high Mg/Ca ratios (4 and 15), in the presence of halophilic bacteria. Moreover, results indicate that supersaturation with respect to Mg (Mg/Ca=15) combined with NaCl (15%) inhibits biomineralization and forms chemical precipitates. 15% salinity is shown to favor chemical precipitation of mineral phases more than 8% salinity. At 10oC, struvite dominates the Mg/Ca=4 system, contrary to same conditions at 30oC. Long-term experiments (Mg/Ca=4, 15% NaCl) showed that, dolomite precipitation is favored over time with elevated pH values (pH: 8-9). Key words: Lake Acıgöl, enrichment cultures, halophilic bacteria, autogenic carbonate, biomineralization, Mg/Ca ratio. References Nurgul Balci, Meryem Menekşe, Nevin Gül Karagüler, M. Şeref Sönmez,Patrick Meister 2015.Reproducing authigenic carbonate precipitation inthe hypersaline Lake Acıgöl (Turkey) with microbial cultures. Geomicrobiology Journal DOI: 10.1080/01490451.2015.1099763. TUBITAK (The Scientific and Technological Research Council of Turkey) Grant to N. BALCI (113Y464).

Effect of hypersaline cooling canals on aquifer salinization

USGS Publications Warehouse

Hughes, Joseph D.; Langevin, Christian D.; Brakefield-Goswami, Linzy

2010-01-01

The combined effect of salinity and temperature on density-driven convection was evaluated in this study for a large (28 km2) cooling canal system (CCS) at a thermoelectric power plant in south Florida, USA. A two-dimensional cross-section model was used to evaluate the effects of hydraulic heterogeneities, cooling canal salinity, heat transport, and cooling canal geometry on aquifer salinization and movement of the freshwater/saltwater interface. Four different hydraulic conductivity configurations, with values ranging over several orders of magnitude, were evaluated with the model. For all of the conditions evaluated, aquifer salinization was initiated by the formation of dense, hypersaline fingers that descended downward to the bottom of the 30-m thick aquifer. Saline fingers reached the aquifer bottom in times ranging from a few days to approximately 5 years for the lowest hydraulic conductivity case. Aquifer salinization continued after saline fingers reached the aquifer bottom and coalesced by lateral movement away from the site. Model results showed that aquifer salinization was most sensitive to aquifer heterogeneity, but was also sensitive to CCS salinity, temperature, and configuration.

Effects of temperature and salinity on light scattering by water

NASA Astrophysics Data System (ADS)

Zhang, Xiaodong; Hu, Lianbo

2010-04-01

A theoretical model on light scattering by water was developed from the thermodynamic principles and was used to evaluate the effects of temperature and salinity. The results agreed with the measurements by Morel within 1%. The scattering increases with salinity in a non-linear manner and the empirical linear model underestimate the scattering by seawater for S < 40 psu. Seawater also exhibits an 'anomalous' scattering behavior with a minimum occurring at 24.64 °C for pure water and this minimum increases with the salinity, reaching 27.49 °C at 40 psu.

Toxicity of the molybdate anion in soil is partially explained by effects of the accompanying cation or by soil pH.

PubMed

Buekers, Jurgen; Mertens, Jelle; Smolders, Erik

2010-06-01

Previous studies have shown that toxicity of cationic trace metals in soil is partially confounded by effects of the accompanying anions. A similar assessment is reported here for toxicity of an oxyanion, i.e., molybdate (MoO(4) (2-)), the soil toxicity of which is relatively unexplored. Solubility and toxicity were compared between the soluble sodium molybdate (Na(2)MoO(4)) and the sparingly soluble molybdenum trioxide (MoO(3)). Confounding effects of salinity were excluded by referencing the Na(2)MoO(4) effect to that of sodium chloride (NaCl). The pH decrease from the acid MoO(3) amendment was equally referenced to a hydrochloric (HCl) treatment or a lime-controlled MoO(3) treatment. The concentrations of molybdenum (Mo) in soil solution or calcium chloride (CaCl(2)) 0.01 M extracts were only marginally affected by either MoO(3) or Na(2)MoO(4) as an Mo source after 10 to 13 days of equilibration. Effects of Mo on soil nitrification were fully confounded by associated changes in salinity or pH. Effects of Mo on growth of wheat seedlings (Triticum aestivum L) were more pronounced than those on nitrification, and toxicity thresholds were unaffected by the form of added Mo. The Mo thresholds for wheat growth were not confounded by pH or salinity at incipient toxicity. It is concluded that oxyanion toxicity might be confounded in relatively insensitive tests for which reference treatments should be included. Copyright 2010 SETAC.

Biodegradation of BTEX mixture by Pseudomonas putida YNS1 isolated from oil-contaminated soil.

PubMed

You, Youngnam; Shim, Jaehong; Cho, Choa-Hyoung; Ryu, Moon-Hee; Shea, Patrick J; Kamala-Kannan, Seralathan; Chae, Jong-Chan; Oh, Byung-Taek

2013-05-01

The presence of mixed contaminants, such as BTEX (benzene, toluene, ethylbenzene and xylene isomers) can affect the biodegradation, fate and environmental impacts of each compound. To understand the influence of interactions among BTEX compounds on their biodegradation, four bacteria were isolated from oil-contaminated soil and assayed for BTEX biodegradation in vitro. The isolate exhibiting maximum biodegradation was identified as Pseudomonas putida based on the 16S rDNA sequence. The biodegradation of the BTEX compounds was greatly influenced by pH, temperature, and salinity. Substrate mixture studies (binary, tertiary and quaternary) revealed that the presence of toluene increased the biodegradation rate of benzene, ethylbenzene, and xylene. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Synthesis, Production, and Biotechnological Applications of Exopolysaccharides and Polyhydroxyalkanoates by Archaea

PubMed Central

Poli, Annarita; Di Donato, Paola; Abbamondi, Gennaro Roberto; Nicolaus, Barbara

2011-01-01

Extreme environments, generally characterized by atypical temperatures, pH, pressure, salinity, toxicity, and radiation levels, are inhabited by various microorganisms specifically adapted to these particular conditions, called extremophiles. Among these, the microorganisms belonging to the Archaea domain are of significant biotechnological importance as their biopolymers possess unique properties that offer insights into their biology and evolution. Particular attention has been devoted to two main types of biopolymers produced by such peculiar microorganisms, that is, the extracellular polysaccharides (EPSs), considered as a protection against desiccation and predation, and the endocellular polyhydroxyalkanoates (PHAs) that provide an internal reserve of carbon and energy. Here, we report the composition, biosynthesis, and production of EPSs and PHAs by different archaeal species. PMID:22007151

Cultivation Of Microalgae (Chlorella vulgaris) For Biodiesel Production

NASA Astrophysics Data System (ADS)

Blinová, Lenka; Bartošová, Alica; Gerulová, Kristína

2015-06-01

Production of biofuel from renewable sources is considered to be one of the most sustainable alternatives to petroleum sourced fuels. Biofuels are also viable means of environmental and economic sustainability. Biofuels are divided into four generations, depending on the type of biomass used for biofuels production. At present, microalgae are presented as an ideal third generation biofuel feedstock because of their rapid growth rate. They also do not compete with food or feed crops, and can be produced on non-arable land. Cultivation conditions (temperature, pH, light, nutrient quantity and quality, salinity, aerating) are the major factors that influence photosynthesis activity and behaviour of the microalgae growth rate. In this paper, we present an overview about the effect of cultivation conditions on microalgae growth.

Biosorption studies on copper (II) and cadmium (II) using pretreated rice straw and rice husk.

PubMed

Li, W C; Law, F Y; Chan, Y H M

2017-04-01

This study investigated the adsorption and removal behaviour of copper (Cu) (II) and cadmium (Cd) (II) ions using rice husk and rice straw in aqueous solutions. Different parameters were used to investigate their adsorption performance in saline conditions and the optimal level of biosorption at different pH levels. The main parameters were pH (3, 6 and 9), initial concentration level of heavy metals (Cu (II) 5, 10, 20, 40 and 60 mg/L and Cd (II) 0.5, 1, 2, 4 and 8 mg/L, respectively), salinity (0, 50 and 100 mM NaCl) and contact time (ranging from 3 to 60 min). Langmuir and Freundlich isotherm models were applied to analyse the removal efficiency and sorption capacity of the pretreated rice husk and rice straw. The removal efficiency and adsorption capacity generally increased with the pH and reached a plateau in alkaline conditions. The percentage removal of Cu (II) by rice husk reached 97 % at pH 9 and 95 % by rice straw at pH 6. Biosorption performance increased in the absence of NaCl. Kinetic studies for both metals revealed that the biosorption of Cu (II) and Cd (II) onto rice straw and husk was pseudo-second order.

Brain temperature changes during selective cooling with endovascular intracarotid cold saline infusion: simulation using human data fitted with an integrated mathematical model.

PubMed

Neimark, Matthew Aaron Harold; Konstas, Angelos Aristeidis; Lee, Leslie; Laine, Andrew Francis; Pile-Spellman, John; Choi, Jae

2013-03-01

The feasibility of rapid cerebral hypothermia induction in humans with intracarotid cold saline infusion (ICSI) was investigated using a hybrid approach of jugular venous bulb temperature (JVBT) sampling and mathematical modeling of transient and steady state brain temperature distribution. This study utilized both forward mathematical modeling, in which brain temperatures were predicted based on input saline temperatures, and inverse modeling, where brain temperatures were inferred based on JVBT. Changes in ipsilateral anterior circulation territory temperature (IACT) were estimated in eight patients as a result of 10 min of a cold saline infusion of 33 ml/min. During ICSI, the measured JVBT dropped by 0.76±0.18°C while the modeled JVBT decreased by 0.86±0.18°C. The modeled IACT decreased by 2.1±0.23°C. In the inverse model, IACT decreased by 1.9±0.23°C. The results of this study suggest that mild cerebral hypothermia can be induced rapidly and safely with ICSI in the neuroangiographical setting. The JVBT corrected mathematical model can be used as a non-invasive estimate of transient and steady state cerebral temperature changes.

A halotolerant thermostable lipase from the marine bacterium Oceanobacillus sp. PUMB02 with an ability to disrupt bacterial biofilms

PubMed Central

Seghal Kiran, George; Nishanth Lipton, Anuj; Kennedy, Jonathan; Dobson, Alan DW; Selvin, Joseph

2014-01-01

A halotolerant thermostable lipase was purified and characterized from the marine bacterium Oceanobacillus sp. PUMB02. This lipase displayed a high degree of stability over a wide range of conditions including pH, salinity, and temperature. It was optimally active at 30 °C and pH 8.0 respectively and was stable at higher temperatures (50–70 °C) and alkaline pH. The molecular mass of the lipase was approximately 31 kDa based on SDS-PAGE and MALDI-ToF fingerprint analysis. Conditions for enhanced production of lipase by Oceanobacillus sp. PUMB02 were attained in response surface method-guided optimization with factors such as olive oil, sucrose, potassium chromate, and NaCl being evaluated, resulting in levels of 58.84 U/ml being achieved. The biofilm disruption potential of the PUMB02 lipase was evaluated and compared with a marine sponge metagenome derived halotolerant lipase Lpc53E1. Good biofilm disruption activity was observed with both lipases against potential food pathogens such as Bacillus cereus MTCC1272, Listeria sp. MTCC1143, Serratia sp. MTCC4822, Escherichia coli MTCC443, Pseudomonas fluorescens MTCC1748, and Vibrio parahemolyticus MTCC459. Phase contrast microscopy, scanning electron microscopy, and confocal laser scanning microscopy showed very effective disruption of pathogenic biofilms. This study reveals that marine derived hydrolytic enzymes such as lipases may have potential utility in inhibiting biofilm formation in a food processing environment and is the first report of the potential application of lipases from the genus Oceanobacillus in biofilm disruption strategies. PMID:25482232

Characterization of saline groundwater across the coastal aquifer of Israel as resource for desalination

NASA Astrophysics Data System (ADS)

Stein, Shaked; Russak, Amos; Sivan, Orit; Yechieli, Yospeh; Oren, Yoram; Kasher, Roni

2015-04-01

In arid countries with access to marine water seawater desalination is becoming an important water source in order to deal with the water scarcity and population growth. Seawater reverse osmosis (RO) facilities use open seawater intake, which requires pretreatment processes to remove particles in order to avoid fouling of the RO membrane. In small and medium size desalination facilities, an alternative water source can be saline groundwater in coastal aquifers. Using saline groundwater from boreholes near the shore as feed water may have the advantage of natural filtration and low organic content. It will also reduce operation costs of pretreatment. Another advantage of using groundwater is its availability in highly populated areas, where planning of large RO desalination plants is difficult and expensive due to real-estate prices. Pumping saline groundwater underneath the freshwater-seawater interface (FSI) might shift the interface towards the sea, thus rehabilitating the fresh water reservoirs in the aquifer. In this research, we tested the potential use of saline groundwater in the coastal aquifer of Israel as feed water for desalination using field work and desalination experiments. Specifically, we sampled the groundwater from a pumping well 100 m from the shore of Tel-Aviv and sea water from the desalination plant in Ashqelon, Israel. We used an RO cross flow system in a pilot plant in order to compare between the two water types in terms of permeate flux, permeate flux decline, salt rejection of the membrane and the fouling on the membrane. The feed, brine and fresh desalinated water from the outlet of the desalination system were chemically analyzed and compared. Field measurements of dissolved oxygen, temperature, pH and salinity were also conducted in situ. Additionally, SDI (silt density index), which is an important index for desalination, and total organic carbon that has a key role in organic fouling and development of biofouling, were measured and compared. The results have shown that using saline groundwater underneath the FSI as a resource for RO desalination process is beneficial in terms of fluxes: the flux reduction in the seawater desalination was 16% of the initial flux, while the flux reduction with the saline groundwater was only 9%. The SDI and total organic carbon were lower in saline groundwater than in seawater, which support the flux results. Therefore, using saline groundwater as feed water for desalination may be advantageous because of lower operational costs and reduced applied pressure needed and energy usage.

The salinity effect in a mixed layer ocean model

NASA Technical Reports Server (NTRS)

Miller, J. R.

1976-01-01

A model of the thermally mixed layer in the upper ocean as developed by Kraus and Turner and extended by Denman is further extended to investigate the effects of salinity. In the tropical and subtropical Atlantic Ocean rapid increases in salinity occur at the bottom of a uniformly mixed surface layer. The most significant effects produced by the inclusion of salinity are the reduction of the deepening rate and the corresponding change in the heating characteristics of the mixed layer. If the net surface heating is positive, but small, salinity effects must be included to determine whether the mixed layer temperature will increase or decrease. Precipitation over tropical oceans leads to the development of a shallow stable layer accompanied by a decrease in the temperature and salinity at the sea surface.

Application of Polar Organic Chemical Integrative Sampler (POCIS) to monitor emerging contaminants in tropical waters.

PubMed

Bayen, Stéphane; Segovia, Elvagris; Loh, Lay Leng; Burger, David F; Eikaas, Hans S; Kelly, Barry C

2014-06-01

Tools specifically validated for tropical environments are needed to accurately describe the behavior of chemical contaminants in tropical ecosystems. In the present study, sampling rates (Rs) were determined for the commercial pharmaceutical-type Polar Organic Chemical Integrative Sampler (POCIS) with a 45.8cm(2) exposure surface for 35 Pharmaceutically Active Compounds (PhACs) and Endocrine Disrupting Compounds (EDCs), of which eight compounds (albuterol, atorvastatin, diltiazem, dilantin, enalapril, norfluoxetine, risperidone and warfarin) were reported for the first time. These sampling rates were measured in an outdoor laboratory calibration setup to best capture diurnal tropical temperature variations (29±3°C). The effect of stirring and salinity was investigated. For all compounds, the sampling rates were higher under stirred conditions as compared to quiescent conditions. Calibration results in the presence of 30g sodium chloride support that the effects of salinity on POCIS sampling rates are compound-specific. Comparisons between Time-Weight Average (TWA) water concentrations using POCIS and spot sample levels in the field (2 lake and 1 mangrove estuary sites) are presented. Results showed that POCIS TWA concentrations were in agreement with spot sample concentrations for these aquatic systems. Results indicate that POCIS can be used to effectively measure the TWA concentration for a range of PhACs and EDCs in tropical waters. However, based on the results from mass balance and field deployments, POCIS did not appear suitable for compounds with a low mass balance recovery during calibration (e.g. triclosan and linuron in this study). Copyright © 2014 Elsevier B.V. All rights reserved.

Bacillus flexus strain As-12, a new arsenic transformer bacterium isolated from contaminated water resources.

PubMed

Jebeli, Mohammad Ahmadi; Maleki, Afshin; Amoozegar, Mohammad Ali; Kalantar, Enayatollah; Izanloo, Hassan; Gharibi, Fardin

2017-02-01

A total of 14 arsenic-resistant bacteria were isolated from an arsenic-contaminated travertine spring water in the central district of Qorveh county, Kurdistan Province, Iran. One of strains designated As-12 was selected for further investigation because of its ability to transform arsenic. The strain was identified by cultural, morphological and biochemical tests, and 16S rRNA gene sequencing. Finally, the growth characteristics of the isolate were investigated in a chemically defined medium which included varied ranges of environmental factors such as pH, temperature and salinity. Moreover, the resistance of this strain to some heavy metals was evaluated. The bacterium was a Gram-positive, endospore-forming with all other characteristics of the genus Bacillus. It revealed maximum similarity at the 16S rRNA gene level with Bacillus flexus. The optimum growth of the strain was observed at 38 °C, pH 9 and 2% salinity. This strain was resistant to heavy metals such as zinc, chromium, lead, nickel, copper, mercuric and cadmium at concentrations of 15 mM, 15.5 mM, 11.5 mM, 12 mM, 11 mM, 5.5 mM, and 1 mM, respectively. The isolated bacterium was able to reduce As (V) to As (III) (about 28%) and oxidize As (III) to As (V) (about 45%) after 48 h of incubation at 37 °C. In conclusion, Bacillus flexus strain As-12, was identified as an arsenic transformer, for the first time. Copyright © 2016 Elsevier Ltd. All rights reserved.

Decadal Variability of Temperature and Salinity in the Northwest Atlantic Ocean

NASA Astrophysics Data System (ADS)

Mishonov, A. V.; Seidov, D.; Reagan, J. R.; Boyer, T.; Parsons, A. R.

2017-12-01

There are only a few regions in the World Ocean where the density of observations collected over the past 60 years is sufficient for reliable data mapping with spatial resolutions finer than one-degree. The Northwest Atlantic basin is one such regions where a spatial resolution of gridded temperature and salinity fields, comparable to those generated by eddy-resolving numerical models of ocean circulation, has recently becomes available. Using the new high-resolution Northwest Atlantic Regional Climatology, built on quarter-degree and one-tenth-degree resolution fields, we analyzed decadal variability and trends of temperature and salinity over 60 years in the Northwest Atlantic, and two 30-year ocean climates of 1955-1984 and 1985-2012 to evaluate the oceanic climate shift in this region. The 30-year climate shift is demonstrated using an innovative 3-D visualization of temperature and salinity. Spatial and temporal variability of heat accumulation found in previous research of the entire North Atlantic Ocean persists in the Northwest Atlantic Ocean. Salinity changes between two 30-year climates were also computed and are discussed.

Simulation of submarine groundwater discharge salinity and temperature variations: Implications for remote detection

USGS Publications Warehouse

Dausman, A.M.; Langevin, C.D.; Sukop, M.C.

2007-01-01

A hydrological analysis using a numerical simulation was done to identify the transient response of the salinity and temperature of submarine groundwater discharge (SGD) and utilize the results to guide data collection. Results indicate that the amount of SGD fluctuates depending on the ocean stage and geology, with the greatest amount of SGD delivered at low tide when the aquifer is in direct hydraulic contact with the ocean. The salinity of SGD remains lower than the ocean throughout the year; however, the salinity difference between the aquifer and ocean is inversely proportional to the ocean stage. The temperature difference between the ocean and SGD fluctuates seasonally, with the greatest temperature differences occurring in summer and winter. The outcome of this research reveals that numerical modelling could potentially be used to guide data collection including aerial surveys using electromagnetic (EM) resistivity and thermal imagery.

Escherichia coli O157:H7 bacteriophage (phi)241 isolated from an industrial cucumber fermentation at high acidity and salinity

USDA-ARS?s Scientific Manuscript database

A novel phage, (phi)241, specific for Escherichia coli O157:H7 was isolated from an industrial cucumber fermentation where both acidity (pH less than or equal to 3.7) and salinity (greater than or equal to 5% NaCl) were high. The phage belongs to the Myoviridae family. Its latent period was 15 min a...

Effects of Precipitation on Ocean Mixed-Layer Temperature and Salinity as Simulated in a 2-D Coupled Ocean-Cloud Resolving Atmosphere Model

NASA Technical Reports Server (NTRS)

Li, Xiaofan; Sui, C.-H.; Lau, K-M.; Adamec, D.

1999-01-01

A two-dimensional coupled ocean-cloud resolving atmosphere model is used to investigate possible roles of convective scale ocean disturbances induced by atmospheric precipitation on ocean mixed-layer heat and salt budgets. The model couples a cloud resolving model with an embedded mixed layer-ocean circulation model. Five experiment are performed under imposed large-scale atmospheric forcing in terms of vertical velocity derived from the TOGA COARE observations during a selected seven-day period. The dominant variability of mixed-layer temperature and salinity are simulated by the coupled model with imposed large-scale forcing. The mixed-layer temperatures in the coupled experiments with 1-D and 2-D ocean models show similar variations when salinity effects are not included. When salinity effects are included, however, differences in the domain-mean mixed-layer salinity and temperature between coupled experiments with 1-D and 2-D ocean models could be as large as 0.3 PSU and 0.4 C respectively. Without fresh water effects, the nocturnal heat loss over ocean surface causes deep mixed layers and weak cooling rates so that the nocturnal mixed-layer temperatures tend to be horizontally-uniform. The fresh water flux, however, causes shallow mixed layers over convective areas while the nocturnal heat loss causes deep mixed layer over convection-free areas so that the mixed-layer temperatures have large horizontal fluctuations. Furthermore, fresh water flux exhibits larger spatial fluctuations than surface heat flux because heavy rainfall occurs over convective areas embedded in broad non-convective or clear areas, whereas diurnal signals over whole model areas yield high spatial correlation of surface heat flux. As a result, mixed-layer salinities contribute more to the density differences than do mixed-layer temperatures.

High-dose diazepam facilitates core cooling during cold saline infusion in healthy volunteers.

PubMed

Hostler, David; Northington, William E; Callaway, Clifton W

2009-08-01

Studies have suggested that inducing mild hypothermia improves neurologic outcomes after traumatic brain injury, major stroke, cardiac arrest, or exertional heat illness. While infusion of cold normal saline is a simple and inexpensive method for reducing core temperature, human cold-defense mechanisms potentially make this route stressful or ineffective. We hypothesized that intravenous administration of diazepam during a rapid infusion of 30 mL.kg-1 of cold (4 degrees C) 0.9% saline to healthy subjects would be more comfortable and reduce core body temperature more than the administration of cold saline alone. Fifteen subjects received rapidly infused cold (4 degrees C) 0.9% saline. Subjects were randomly assigned to receive, intravenously, 20 mg diazepam (HIGH), 10 mg diazepam (LOW), or placebo (CON). Main outcomes were core temperature, skin temperature, and oxygen consumption. Data for the main outcomes were analyzed with generalized estimating equations to identify differences in group, time, or a group x time interaction. Core temperature decreased in all groups (CON, 1.0 +/- 0.2 degrees C; LOW, 1.4 +/- 0.2 degrees C; HIGH, 1.5 +/- 0.2 degrees C), while skin temperature was unchanged. Mean (95% CI) oxygen consumption was 315.3 (253.8, 376.9) mL.kg-1.min-1 in the CON group, 317.9 (275.5, 360.3) in the LOW group, and 226.1 (216.4, 235.9) in the HIGH group. Significant time and group x time interaction was observed for core temperature and oxygen consumption (p < 0.001). Administration of high-dose diazepam resulted in decreased oxygen consumption during cold saline infusion, suggesting that 20 mg of intravenous diazepam may reduce the shivering threshold without compromising respiratory or cardiovascular function.

Cerebral Metabolic Alterations in Rats With Diabetic Ketoacidosis

PubMed Central

Glaser, Nicole; Yuen, Natalie; Anderson, Steven E.; Tancredi, Daniel J.; O'Donnell, Martha E.

2010-01-01

OBJECTIVE Cerebral edema is a life-threatening complication of diabetic ketoacidosis (DKA) in children. Recent data suggest that cerebral hypoperfusion and activation of cerebral ion transporters may be involved, but data describing cerebral metabolic alterations during DKA are lacking. RESEARCH DESIGN AND METHODS We evaluated 50 juvenile rats with DKA and 21 normal control rats using proton and phosphorus magnetic resonance spectroscopy (MRS). MRS measured cerebral intracellular pH and ratios of metabolites including ATP/inorganic phosphate (Pi), phosphocreatine (PCr)/Pi, N-acetyl aspartate (NAA)/creatine (Cr), and lactate/Cr before and during DKA treatment. We determined the effects of treatment with insulin and intravenous saline with or without bumetanide, an inhibitor of Na-K-2Cl cotransport, using ANCOVA with a 2 × 2 factorial study design. RESULTS Cerebral intracellular pH was decreased during DKA compared with control (mean ± SE difference −0.13 ± 0.03; P < 0.001), and lactate/Cr was elevated (0.09 ± 0.02; P < 0.001). DKA rats had lower ATP/Pi and NAA/Cr (−0.32 ± 0.10, P = 0.003, and −0.14 ± 0.04, P < 0.001, respectively) compared with controls, but PCr/Pi was not significantly decreased. During 2-h treatment with insulin/saline, ATP/Pi, PCr/Pi, and NAA/Cr declined significantly despite an increase in intracellular pH. Bumetanide treatment increased ATP/Pi and PCr/Pi and ameliorated the declines in these values with insulin/saline treatment. CONCLUSIONS These data demonstrate that cerebral metabolism is significantly compromised during DKA and that further deterioration occurs during early DKA treatment—consistent with possible effects of cerebral hypoperfusion and reperfusion injury. Treatment with bumetanide may help diminish the adverse effects of initial treatment with insulin/saline. PMID:20028943

Chesapeake Bay Low Freshwater Inflow Study. Biota Assessment. Phase I. Volume II.

DTIC Science & Technology

1980-08-01

which can regulate in water of reduced salinity , but not fresh water, e holeuryhaline osmoregulators , which can regulate from fresh to full oceanic...salini- ties , and * oligohaline osmoregulators , which can regulate only in fresh water and very low salinities , and maintain blood hyperosmotic to the...areas, or oysters their upstream beds. Temperature: The synergistic effects of temperature and salinity have been described by Kinne (1963, 1964) and

Optimization of Fermentation Conditions and Rheological Properties of Exopolysaccharide Produced by Deep-Sea Bacterium Zunongwangia profunda SM-A87

PubMed Central

Liu, Sheng-Bo; Qiao, Li-Ping; He, Hai-Lun; Zhang, Qian; Chen, Xiu-Lan; Zhou, Wei-Zhi; Zhou, Bai-Cheng; Zhang, Yu-Zhong

2011-01-01

Zunongwangia profunda SM-A87 isolated from deep-sea sediment can secrete large quantity of exopolysaccharide (EPS). Response surface methodology was applied to optimize the culture conditions for EPS production. Single-factor experiment showed that lactose was the best carbon source. Based on the Plackett–Burman design, lactose, peptone and temperature were selected as significant variables, which were further optimized by the steepest ascent (descent) method and central composite design. The optimal culture conditions for EPS production and broth viscosity were determined as 32.21 g/L lactose, 8.87 g/L peptone and an incubation temperature of 9.8°C. Under these conditions, the maximum EPS yield and broth viscosity were 8.90 g/L and 6551 mPa•s, respectively, which is the first report of such high yield of EPS from a marine bacterium. The aqueous solution of the EPS displayed high viscosity, interesting shearing thinning property and great tolerance to high temperature, a wide range of pH, and high salinity. PMID:22096500

Preparation of thermo-responsive membranes. II.

PubMed

Nozawa, I; Suzuki, Y; Sato, S; Sugibayashi, K; Morimoto, Y

1991-05-01

Two types of liquid crystal (LC)-immobilized membranes were prepared by a soaking method and sandwich method to control the permeation of indomethacin, as a model drug, in response to local and systemic fever. Monooxyethylene trimethylolpropane tristearate (MTTS) was used as a model LC because it has a gel-liquid crystal phase transition temperature near the body temperature, 39-40 degrees C in phosphate buffered saline (pH 7.4). Two porous polypropylene (PP) membranes were soaked into 20% MTTS chloroform solution in the soaking method, and two PP membranes were poured with the melted MTTS and pressed in the sandwich method. Thermo-response efficacy of the soaked membrane was dependent upon the content of MTTS in MTTS membrane, and the MTTS content above the void volume of PP membrane (38%) was needed for high efficacy. On the other hand, the sandwich membrane exhibited higher thermo-response efficacy than the soaked membrane, because more LC was embedded in the pores of sandwich membrane than that of the soaked membrane. The sandwich membrane permeation of indomethacin was sharply controlled by temperature changes between 32 and 38 degrees C.

Effectiveness of the Surfactant Dioctyl Sodium Sulfosuccinate (DOSS) to Disperse Oil in a Changing Marine Environment

NASA Astrophysics Data System (ADS)

Steffy, D. A.; Nichols, A.; Kiplagat, G.

2011-12-01

We investigated the surfactant which was used to disperse the oil spill which occurred in the Gulf of Mexico during the summer 2010. The surfactant DOSS is an organic sulfonic acid salt which is a synthetic detergent that disrupts the interfacial tension between the saltwater and crude oil phases. The disruption becomes maximum at or above the critical micelle concentration (CMC). The CMC for the surfactant was determined to be at 0.13 % solution in deionized water at a pH of 7.2 and a temperature of 70oF. The CMC is lower at 0.09% solution in salt water. The effect has been identified as a "salting out" effect (Somasundaran, 2006). The CMC of DOSS in both saline and deionized water occurred at lower percent solutions at higher temperatures. The surface tension versus % solution plots are modeled by a power equation, with correlation coefficients consistently over 0.94. Surface tension versus percent solution plots are scalable to fit a temperature desired by the function f(x)= (1/(1+X^α)), where α = T1/T2.

Abiotic factors influencing biomass accumulation of green tide causing Ulva spp. on Pyropia culture rafts in the Yellow Sea, China.

PubMed

Keesing, John K; Liu, Dongyan; Shi, Yajun; Wang, Yujue

2016-04-15

Annually recurrent green-tides in the Yellow Sea have been shown to result from direct disposal into the sea of fouling Ulva from Pyropia aquaculture. The role abiotic factors play in Ulva biomass accumulation on rafts was studied to find ways to mitigate this problem. Dissolved inorganic nitrogen (DIN) was very high at all sites, but the highest Ulva biomass was associated with the lowest DIN and anthropogenic N. Under luxuriant background nutrient conditions, variability in temperature and periods of emersion, rather than pH, light and salinity determined Ulva biomass. Two dominant species of Ulva displayed differing tolerances to temperature and desiccation which helped explain why Ulva prolifera dominates floating green-tides. Rather than trying to mitigate green-tides only by reducing nutrient pollution, an earlier harvest of Pyropia in southern Jiangsu Province especially before temperatures increase greatly above 10°C during April, could reduce the biomass of U. prolifera disposed from rafts. Crown Copyright © 2016. Published by Elsevier Ltd. All rights reserved.

Development and Testing of a Coupled Ocean-atmosphere Mesoscale Ensemble Prediction System

DTIC Science & Technology

2011-06-28

wind, temperature, and moisture variables, while the oceanographic ET is derived from ocean current, temperature, and salinity variables. Estimates of...wind, temperature, and moisture variables while the oceanographic ET is derived from ocean current temperature, and salinity variables. Estimates of...uncertainty in the model. Rigorously accurate ensemble methods for describing the distribution of future states given past information include particle

Structural adaptations of octaheme nitrite reductases from haloalkaliphilic Thioalkalivibrio bacteria to alkaline pH and high salinity.

PubMed

Popinako, Anna; Antonov, Mikhail; Tikhonov, Alexey; Tikhonova, Tamara; Popov, Vladimir

2017-01-01

Bacteria Tv. nitratireducens and Tv. paradoxus from soda lakes grow optimally in sodium carbonate/NaCl brines at pH range from 9.5 to 10 and salinity from 0.5 to 1.5 M Na+. Octaheme nitrite reductases (ONRs) from haloalkaliphilic bacteria of genus Thioalkalivibrio are stable and active in a wide range of pH (up to 11) and salinity (up to 1 M NaCl). To establish adaptation mechanisms of ONRs from haloalkaliphilic bacteria a comparative analysis of amino acid sequences and structures of ONRs from haloalkaliphilic bacteria and their homologues from non-halophilic neutrophilic bacteria was performed. The following adaptation strategies were observed: (1) strategies specific for halophilic and alkaliphilic proteins (an increase in the number of aspartate and glutamate residues and a decrease in the number of lysine residues on the protein surface), (2) strategies specific for halophilic proteins (an increase in the arginine content and a decrease in the number of hydrophobic residues on the solvent-accessible protein surface), (3) strategies specific for alkaliphilic proteins (an increase in the area of intersubunit hydrophobic contacts). Unique adaptation mechanism inherent in the ONRs from bacteria of genus Thioalkalivibrio was revealed (an increase in the core in the number of tryptophan and phenylalanine residues, and an increase in the number of small side chain residues, such as alanine and valine, in the core).

Ability of a haloalkaliphilic bacterium isolated from Soap Lake, Washington to generate electricity at pH 11.0 and 7% salinity.

PubMed

Paul, Varun G; Minteer, Shelley D; Treu, Becky L; Mormile, Melanie R

2014-01-01

A variety of anaerobic bacteria have been shown to transfer electrons obtained from organic compound oxidation to the surface of electrodes in microbial fuel cells (MFCs) to produce current. Initial enrichments for iron (III) reducing bacteria were set up with sediments from the haloalkaline environment of Soap Lake, Washington, in batch cultures and subsequent transfers resulted in a culture that grew optimally at 7.0% salinity and pH 11.0. The culture was used to inoculate the anode chamber of a MFC with formate as the electron source. Current densities up to 12.5 mA/m2 were achieved by this bacterium. Cyclic voltammetry experiments demonstrated that an electron mediator, methylene blue, was required to transfer electrons to the anode. Scanning electron microscopic imaging of the electrode surface did not reveal heavy colonization of bacteria, providing evidence that the bacterium may be using an indirect mode of electron transfer to generate current. Molecular characterization of the 16S rRNA gene and restriction fragment length profiles (RFLP) analysis showed that the MFC enriched for a single bacterial species with a 99% similarity to the 16S rRNA gene of Halanaerobium hydrogeniformans. Though modest, electricity production was achieved by a haloalkaliphilic bacterium at pH 11.0 and 7.0% salinity.

Biomineralization in foraminifera

NASA Astrophysics Data System (ADS)

Nooijer, L. D.; Toyofuku, T.; Bijma, J.; Reichart, G. J.

2015-12-01

Foraminifera are popular tools in paleoceanography since incorporation of minor/ major elements and fractionation of stable isotopes into their carbonate shells depend on environmental conditions (e.g. temperature, salinity, pH). Their shell chemistry is markedly different from that of inorganically precipitated CaCO3, reflecting that calcification is a process under strong biological control. The cellular components responsible for calcification are only partly identified in foraminifera and include the involvement of organic templates, trans-membrane ion transporters and selective ion removal. Recent results suggest that transmembrane exchange of H+ for Ca2+ is directly responsible for calcification. The resulting high pH inside and lowered pH outside the foraminifer results in an efficient CO2 'trap' after which carbon dioxide is converted to carbonate prior to calcification. Amongst others, this explains how some foraminifera are able to calcify in undersaturated seawater and may explain their moderate response to ocean acidification. Minor and trace metals incorporated into test carbonate reflect the processes involved in biomineralization and can thus be used to unravel the different factors and processes involved. Still, a more detailed understanding of the processes involved in foraminiferal calcification is needed to explain observed (inter-species) differences in partition coefficients for the incorporation of minor and trace metals and isotopic fractionation.

Research on chemical characteristics of soil salt crusts with saline groundwater drip-irrigation in the Tarim Desert Highway Shelterbelt.

PubMed

Zhang, Jianguo; Xu, Xinwen; Lei, Jiaqiang; Li, Shengyu

2013-01-01

Soil salt crusts are special layers at soil surface which are widely distributed in the Trim Desert Highway Shelterbelt under drip-irrigation with high salinity groundwater. In order to reveal annual variation of their chemical characteristics, soil salt crusts in shelterbelt of different ages in hinterland of the Taklimakan Desert were sampled. SOM, total salt, inions and pH were analyzed. Following results were obtained. SOM of salt crusts increased with the shelterbelt ages, but increasing trend became lower gradually. Total salt, ions, and pH of salt crusts reduced gradually with the shelterbelt ages. Total salt of salt crusts in shelterbelt of different ages was much higher than shifting sandy land. Ions were higher than shifting sandy land, Cl(-), Na(+), and SO4 (2-) increased more obvious, then Mg(2+), K(+), Ca(2+) and HCO3 (-), CO3 (2-) was little and nearly had no change. pH was all alkaline, pH of salt crusts in shelterbelt of 11 years was even lower than shifting sandy land. We can include that the quality of shallow soil (0~30 cm) in the Trim Desert Highway Shelterbelt becomes better gradually.

Preliminary study : optimization of pH and salinity for biosurfactant production from Pseudomonas aeruginosa in diesel fuel and crude oil medium

NASA Astrophysics Data System (ADS)

Ikhwani, A. Z. N.; Nurlaila, H. S.; Ferdinand, F. D. K.; Fachria, R.; Hasan, A. E. Z.; Yani, M.; Setyawati, I.; Suryani

2017-03-01

Biosurfactant is secondary metabolite surface active compound produced by microorganisms which is nontoxic and eco-friendly. Microorganism producing biosurfactant that is quite potential to use in many applications is from Pseudomonas aeruginosa strains. Good quality of biosurfactant production from microbes is supported by the suitable nutrients and environmental factors. The aim of this research was to obtain preliminary o data upon the optimum pH and salinity for the production of biosurfactant from Pseudomonas aeruginosa ATCC 15442 in diesel fuel and crude oil medium. P. aeruginosa ATCC 15442 cultured in diesel fuel and crude oil as carbon source showed biosurfactant activity. P.aeruginosa-derived biosurfactant was capable to form stable emulsion for 24 hours (EI24) in hydrocarbons n-hexane solutions. The particular biosurfactant showed EI24 highest value at pH 7 (31.02%) and 1% NaCl (24.00%) when P. aeruginosa was grown in 10% diesel fuel medium in mineral salt solution. As for the media crude oil, the highest EI24 value was at pH 6 (52.16%) and 1% NaCl (33.30%).

The distribution and origins of extremely acidic saline groundwaters in the south of Western Australia - Groundwater and digital mapping datasets provide new insights

NASA Astrophysics Data System (ADS)

Lillicrap, Adam M.; Biermann, Vera; George, Richard J.; Gray, David J.; Oldham, Carolyn E.

2018-01-01

Some of the largest extents of naturally occurring acidic waters are found across southern Australia. The origins of these systems remain poorly understood with many hypotheses for their genesis. Australian government agency groundwater datasets and mapping data (vegetation, geology, regolith and soils) for south-western Australia, unavailable to previous researchers, were statistically analysed to better understand the origins of acidic groundwater and guide additional fieldwork to study the origins of acidic saline groundwater. The groundwater data showed a distinct bimodal distribution in pH; the 'acid' population had a median pH of 3.5 and the larger 'non-acid' population had a median pH of 6.6. Acidic groundwater became progressively more common further from the coast towards the drier internally drained regions. Acidic groundwater was mostly confined to the lower slopes and valley floors with localised controls on distribution. Paradoxically, subsoil alkalinity within the internally drained inland regions had the strongest correlation with acidic groundwater (r2 = 0.85). Vegetation was also a strong predictor of acidic groundwater. Acidic groundwater had the highest occurrence under Eucalyptus woodlands and shrublands that grew on alkaline calcareous soils. Pre-clearing soil data in areas with acidic saline groundwater showed that the upper 1 m of the unsaturated zone had a pH around 8 while the pH at depths greater than 5 m decreased to <4. Based on the observations it is proposed that biogenic formation of calcareous soils occurs in the upper 1 m of the profile, calcium is sourced from the deeper profile where the root biota exchanges calcium for hydrogen ions to maintain charge balance. Iron is mobilised from the upper soil profile and concentrates lower in the profile at depths >1.5 m. There, the iron is reduced around roots and the alkalinity generated by microbial iron reduction is removed by biogenic calcification processes. The iron moves in solution further down the profile following roots where it comes in contact with the oxygenated unsaturated zone matrix and is oxidised generating acid. The resulting acidic recharging solution acidifies the unsaturated zone matrix. Saline groundwater moving through the matrix becomes acidified due to ion exchange or direct recharge. The main chemical processes were modelled in PHREEQC to test the plausibility of the hypothesis and acidic solutions with a pH of 3.8 or lower were obtained.

Limnology of Kharland (saline) ponds of Ratnagiri, Maharashtra in relation to prawn culture potential.

PubMed

Saksena, D N; Gaidhane, D M; Singh, H

2006-01-01

The coastal saline soils, Kharlands, have great potential for their use in aquaculture. This study has been taken up to understand the limnology of the ponds in Kharland area for assessing their prawn culture potential. This study was carried out during September, 1999 to August, 2001. Each Kharland pond has an area of 0.045 hectare. During the study, depth of pond water was 47.7 to 120.0 cm, temperature varied from 25.7 to 34.5 degrees C; transparency from nil to 65.0 cm; specific conductivity from 1.78 to 94.5 microS.cm(-1); total dissolved solids from 0.89 to 27.55 ppt; pH 5.42 to 8.25; dissolved oxygen 1.6 to 8 mg.l(-1); free carbon dioxide 10.00 to 44.00 mg.l(-1); total alkalinity 5.00 to 142.00 mg.l(-1); salinity 0.45 to 39.55 ppt; total hardness 245.00 to 5945.00; calcium 56.05 to 1827.6; magnesium 110.74 to 4507.75 mg.l(-1); dissolved organic matter 1.45 to 9.68 mg.l(-1); ammonia 1.00-8.00 microg.l(-1); nitrite nil to 20.00 micro l(-1) and nitrate 7.5 to 17.5 microg.l(-1). These Kharland ponds are unique in physio-chemical characteristics during their seasonal cycle. From July to October, these ponds have nearly freshwater while from November to May pond water becomes saline. Thus, there is a great possibility of taking up monoculture of both the freshwater and brackish water prawns as well as polyculture of prawns and fishes in the Kharland ponds.

Dissolved Strontium and Barium in Fresh and Saltwater: a 2-year Study in the Calcasieu River to the Gulf of Mexico

NASA Astrophysics Data System (ADS)

He, S.; Xu, Y. J.

2016-02-01

Strontium and barium to calcium ratios are often used as proxies for tracking animal movement across salinity gradients. As sea level rise continues, many estuarine rivers face saltwater intrusion, which may cause changes in mobility and distribution of these metals upstream. Despite intensive research on metal adsorption and desorption in marine systems, knowledge of the spatiotemporal distribution of these elements along estuarine rivers is still limited. In this study, we conducted an intensive monitoring of Sr and Ba dynamics along an 88-km long estuary, the Calcasieu River, which has been strongly affected by saltwater intrusion. Over the period from May 2013 to July 2015, we collected monthly water samples and performed in-situ water quality measurements at six sites from the upstream to the river mouth. Water samples were analyzed for dissolved Sr, Ba, and Ca concentrations. In-situ measurements of salinity, pH, water temperature, dissolved oxygen concentration, and specific conductance were taken. Our preliminary data showed that the Sr and Ca concentrations and the Sr/Ca ratio all increased significantly with decreasing distance to the Gulf of Mexico, while the Ba/Ca ratio decreased with decreasing distance to the Gulf. The spatial variation in Ba concentration was marginal. The Sr and Ca concentrations and ratios were positively related to salinity, while Ba/Ca was negatively related to salinity. All the elemental concentrations and ratios had considerable seasonal and interannual variations. There were significant differences among sampling months for all the elemental concentrations and ratios (p<0.05), and there were significant differences among sampling years for the Sr and Ca concentrations and the Ba/Ca ratio (p<0.05).

Effects of solutions treated with oxygen radicals in neutral pH region on inactivation of microorganism

NASA Astrophysics Data System (ADS)

Kobayashi, Tsuyoshi; Hashizume, Hiroshi; Ohta, Takayuki; Ishikawa, Kenji; Hori, Masaru; Ito, Masafumi

2015-09-01

The inactivation of microorganisms using nonequilbrium atmospheric pressure plasmas has been attracted much attention due to the low temperature processing and high speed treatment. In this study, we have inactivated E. coli suspended in solutions with neutral pH using an atmospheric-pressure oxygen radical source which can selectively supply electrically neutral oxygen radicals. E. coli cells were suspended with deionized distilled water (DDW) (pH = 6.8) or phosphate buffered saline (PBS) (pH = 7.4) or Citrate-Na buffer (pH = 6.5). The treated samples were diluted and spread on nutrient agar (Nutrient Broth). They were cultured at 37° C. The inactivation effects of oxygen radicals on those cells in solutions were evaluated by colony-counting method. O2 diluted by Ar gas were employed as a working gas for the radical source. The total gas flow rate and the gas mixture ratio of O2/(Ar + O2) were set at 5 slm and 0.6%, respectively. The distance between the radical exit and the suspension surface were set at 10 mm. As a result, the D values for DDW(pH = 6.8), PBS(pH = 7.4) and Citrate-Na buffer(pH = 6.5) were estimated to be 1.4 min, 0.9 min and 16.8 min respectively. The inactivation rates in DDW, PBS were significantly different from that in Citrate-Na buffer. This work was partly supported by JSPS KAKENHI Grant Number 26286072 and project for promoting Research Center in Meijo University.

Salinity controls on Na incorporation in Red Sea planktonic foraminifera

NASA Astrophysics Data System (ADS)

Mezger, E. M.; de Nooijer, L. J.; Boer, W.; Brummer, G. J. A.; Reichart, G. J.

2016-12-01

Whereas several well-established proxies are available for reconstructing past temperatures, salinity remains challenging to assess. Reconstructions based on the combination of (in)organic temperature proxies and foraminiferal stable oxygen isotopes result in relatively large uncertainties, which may be reduced by application of a direct salinity proxy. Cultured benthic and planktonic foraminifera showed that Na incorporation in foraminiferal shell calcite provides a potential independent proxy for salinity. Here we present the first field calibration of such a potential proxy. Living planktonic foraminiferal specimens from the Red Sea surface waters were collected and analyzed for their Na/Ca content using laser ablation quadrupole inductively coupled plasma mass spectrometry. Using the Red Sea as a natural laboratory, the calibration covers a broad range of salinities over a steep gradient within the same water mass. For both Globigerinoides ruber and Globigerinoides sacculifer calcite Na/Ca increases with salinity, albeit with a relatively large intraspecimen and interspecimen variability. The field-based calibration is similar for both species from a salinity of 36.8 up to 39.6, while values for G. sacculifer deviate from this trend in the northernmost transect. It is hypothesized that the foraminifera in the northernmost part of the Red Sea are (partly) expatriated and hence should be excluded from the Na/Ca-salinity calibration. Incorporation of Na in foraminiferal calcite therefore provides a potential proxy for salinity, although species-specific calibrations are still required and more research on the effect of temperature is needed.

The Effects of In-Hospital Intravenous Cold Saline in Postcardiac Arrest Patients Treated with Targeted Temperature Management.

PubMed

Suppogu, Nissi; Panza, Gregory A; Kilic, Sena; Gowdar, Shreyas; Kallur, Kamala R; Jayaraman, Ramya; Lundbye, Justin; Fernandez, Antonio B

2018-03-01

Recent data suggest that rapid infusion of intravenous (IV) cold saline for Targeted Temperature Management (TTM) after cardiac arrest is associated with higher rates of rearrest, pulmonary edema, and hypoxia, with no difference in neurologic outcomes or survival when administered by Emergency Medical Services. We sought to determine the effects of IV cold saline administration in the hospital setting in postcardiac arrest patients to achieve TTM and its effect on clinical parameters and neurologic outcomes. A cohort of 132 patients who completed TTM after cardiac arrest in a single institution was retrospectively studied. Patients who did not receive cold saline were matched by age, gender, Glasgow coma scale, downtime, and presenting rhythm to patients who received cold saline. Demographics, cardiac rearrest, diuretic use, time to target temperature, and Cerebral Performance Category (CPC) scores were recorded among other variables. Patients who received cold saline achieved target temperature sooner (280 vs. 345 minutes, p = 0.05), had lower lactate levels on day 1 (4.2 ± 3.5 mM vs. 6.0 ± 4.9 mM, p = 0.019) and day 2 (1.3 ± 2.2 mM vs. 2.2 ± 3.2 mM, p = 0.046), increased incidence of pulmonary edema (51.5% vs. 31.8%, p = 0.006), and increased diuretic utilization (63.6% vs. 42.4%, p = 0.014). There was no significant difference in cardiac rearrest, arterial oxygenation, and CPC scores (ps > 0.05). Infusion of IV cold saline is associated with shorter time to target temperature, increased incidence of pulmonary edema, and diuretic use, with no difference in cardiac rearrest, survival, and neurologic outcomes.

Advanced purification of petroleum refinery wastewater by catalytic vacuum distillation.

PubMed

Yan, Long; Ma, Hongzhu; Wang, Bo; Mao, Wei; Chen, Yashao

2010-06-15

In our work, a new process, catalytic vacuum distillation (CVD) was utilized for purification of petroleum refinery wastewater that was characteristic of high chemical oxygen demand (COD) and salinity. Moreover, various common promoters, like FeCl(3), kaolin, H(2)SO(4) and NaOH were investigated to improve the purification efficiency of CVD. Here, the purification efficiency was estimated by COD testing, electrolytic conductivity, UV-vis spectrum, gas chromatography-mass spectrometry (GC-MS) and pH value. The results showed that NaOH promoted CVD displayed higher efficiency in purification of refinery wastewater than other systems, where the pellucid effluents with low salinity and high COD removal efficiency (99%) were obtained after treatment, and the corresponding pH values of effluents varied from 7 to 9. Furthermore, environment estimation was also tested and the results showed that the effluent had no influence on plant growth. Thus, based on satisfied removal efficiency of COD and salinity achieved simultaneously, NaOH promoted CVD process is an effective approach to purify petroleum refinery wastewater. Copyright 2010 Elsevier B.V. All rights reserved.

Hematocrit and plasma osmolality values of young-of-year shortnose sturgeon following acute exposures to combinations of salinity and temperature

USGS Publications Warehouse

Ziegeweid, J.R.; Black, M.C.

2010-01-01

Little is known about the physiological capabilities of young-of-year (YOY) shortnose sturgeon. In this study, plasma osmolality and hematocrit values were measured for YOY shortnose sturgeon following 48-h exposures to 12 different combinations of salinity and temperature. Hematocrit levels varied significantly with temperature and age, and plasma osmolalities varied significantly with salinity and age. Plasma osmolality and hematocrit values were similar to previously published values for other sturgeons of similar age and size in similar treatment conditions. ?? 2010 Springer Science+Business Media B.V.

Electrical Impedance Tomography of Electrolysis

PubMed Central

Meir, Arie; Rubinsky, Boris

2015-01-01

The primary goal of this study is to explore the hypothesis that changes in pH during electrolysis can be detected with Electrical Impedance Tomography (EIT). The study has relevance to real time control of minimally invasive surgery with electrolytic ablation. To investigate the hypothesis, we compare EIT reconstructed images to optical images acquired using pH-sensitive dyes embedded in a physiological saline agar gel phantom treated with electrolysis. We further demonstrate the biological relevance of our work using a bacterial E.Coli model, grown on the phantom. The results demonstrate the ability of EIT to image pH changes in a physiological saline phantom and show that these changes correlate with cell death in the E.coli model. The results are promising, and invite further experimental explorations. PMID:26039686

The motility and motion duration of jatimbulan tilapia (Oreochromis niloticus) spermatozoa in different salinity

NASA Astrophysics Data System (ADS)

Triastuti, J.; Kintani, D.; Luqman, E. M.; Pujiastuti, D. Y.

2018-04-01

Tilapia hatchery is still conducted in freshwater and seeds are death simultaneousy when cultivated in high salinity due to the acclimatization process. An alternative method to implement hatchery at high salinity is required. This study aims to determine the salinity of activation medium that provides the best Jatimbulan Tilapia sperm motility and motion duration at high salinity. The study applies completely randomized design (CRD), which consists of 5 treatments (0 ppt, 4 ppt, 9 ppt, 14 ppt and 19 ppt) and 4 repetitions. The parameters consists of sperm motility, motion duration, fresh sperm data (volume, color, odor, pH, consistency, and the concentration of sperm) and sperm abnormalities. The results exhibited that salinity significantly (p < 0.05). Influeneed the sperm motility and motion duration. Motility reaches its best at 0 ppt and 4 ppt (93.4 % and 87.8 %). For motion duration, best condition was in 0 ppt and 4 ppt treatments, totaling 2128 seconds and 1961.5 seconds. Meanwhile, sperm did not move when treated in waters with 9 ppt, 14 ppt and 19 ppt salinities.

The physicochemical and environmental factors affecting the distribution of Anopheles merus along the Kenyan coast.

PubMed

Kipyab, Pamela C; Khaemba, Battan M; Mwangangi, Joseph M; Mbogo, Charles M

2015-04-11

Members of the Anopheles gambiae complex are the main transmitters of malaria. Anopheles merus is a member of the complex found along the Kenyan coast because it breeds in saline waters. An entomological study was conducted in Garithe Malindi District, to investigate the physicochemical and environmental factors affecting the distribution of An. merus. Field and laboratory studies were used to investigate the breeding habitats of the subspecies. Mosquito larvae were sampled using standard dipping technique from small pockets of pools, ponds, hoof prints, road drain, wells and mangrove swamps found in Garithe. All 3(rd) and 4(th) instars of Anopheles larvae sampled were identified microscopically into species. A representative of Anopheles gambiae complex was then identified to specific sibling species using r-DNA PCR technique. The habitats were characterized based on temperature, conductivity, salinity, dissolved oxygen, total dissolved solids, pH, size, distance to nearest house, canopy coverage, surface debris, presence of algae, emergent plants, turbidity and habitat types. A total of 159 morphologically identified late stage instar Anopheles gambiae s.l larvae were selected for r-DNA analysis by PCR. Out of these, 60.4% (n = 96) were Anopheles merus, 8.8% (n = 14) were Anopheles arabiensis, 18.2% (n = 29) were Anopheles gambiae s.s and 12.6% (n = 20) were unknown. Using paired t-test (t (121) = -3.331, P = 0.001) a significantly high proportion of An. merus was observed in all habitats compared to An. arabiensis, and An. gambiae s. s. In habitat characterization, Pearson's correlation analysis test showed different parameters being associated with the occurrence of An. merus larvae in the different habitats sampled. Six out of the 55 correlation coefficients (10.9%) were statistically significant, suggesting non-random association between some pairs of variables. Those that had a significantly high positive correlation with An. merus included temperature, salinity, conductivity, total dissolved solids and algae. Different physicochemical parameters and environmental parameters affect the occurrence of An. merus. In this study, higher temperatures accelerate the growth of the larvae and aids in growth of micro-organisms and algae which are food sources for the larvae. Saline waters favour the growth and development of An. merus larvae; they are also able to develop in a range of saline waters. Conductivity, total dissolved solids and canopy coverage are among the important factors influencing the development and abundance of An. merus larvae in their habitats. Habitat type also influences the abundance of An. merus larvae. They mainly prefer to breed in pools and ponds, but not swamps, hoof prints and wells.

Does thermal carbonization (Biochar) of organic material increase more merits for their amendments of sandy soil?

NASA Astrophysics Data System (ADS)

Wu, Y.; Xu, G.; Sun, J. N.; Shao, H. B.

2014-02-01

Organic materials (e.g. furfural residue) are generally believed to improve the physical and chemical properties of the soils with low fertility. Recently, biochar have been received more attention as a possible measure to improve the carbon balance and improve soil quality in some degraded soils. However, little is known about their different amelioration of a sandy saline soil. In this study, 56d incubation experiment was conducted to evaluate the influence of furfural and its biochar on the properties of saline soil. The results showed that both furfural and biochar greatly reduced pH, increased soil organic carbon (SOC) content and cation exchange capacity (CEC), and enhanced the available phosphorus (P) in the soil. Furfural is more efficient than biochar in reducing pH: 5% furfural lowered the soil pH by 0.5-0.8 (soil pH: 8.3-8.6), while 5% biochar decreased by 0.25-0.4 due to the loss of acidity in pyrolysis process. With respect to available P, 5% of the furfural addition increased available P content by 4-6 times in comparison to 2-5 times with biochar application. In reducing soil exchangeable sodium percentage (ESP), biochar is slightly superior to furfural because soil ESP reduced by 51% and 43% with 5% furfural and 5% biochar addition at the end of incubation. In addition, no significant differences were observed between furfural and biochar about their capacity to retain N, P in leaching solution and to increase CEC in soil. These facts may be caused by the relatively short incubation time. In general, furfural and biochar have different amendments depending on soil properties: furfural was more effectively to decrease pH and to increase available P, whereas biochar played a more important role in increasing SOC and reducing ESP of saline soil.

Effects of calcium, magnesium, and sodium on alleviating cadmium toxicity to Hyalella azteca

USGS Publications Warehouse

Jackson, B.P.; Lasier, P.J.; Miller, W.P.; Winger, P.V.

2000-01-01

Toxicity of trace metal ions to aquatic organisms, arising through either anthropogenic inputs or acidification of surface waters, continues to be both a regulatory and environmental problem. It is generally accepted that the free metal ion is the major toxic species (Florence et a1.,1992) and that inorganic or organic complexation renders the metal ion non-bioavailable (Meador, 1991, Galvez and Wood, 1997). However, water chemistry parameters such as alkalinity, hardness, dissolved organic carbon and pH influence metal ion toxicity either directly by lowering free metal ion concentration or indirectly through synergistic or antagonistic effects. Alkalinity and salinity can affect the speciation of metal ions by increasing ion-pair formation, thus decreasing free metal ion concentration. For example, Cu was found to be less toxic to rainbow trout in waters of high alkalinity (Miller and Mackay, 1980), due to formation of CuCO3 ion pair, and corresponding reduction in free Cu2+ concentration. The influence of salinity on the toxicity of cadmium to various organisms has been demonstrated in a number of studies (Bervoets et al., 1995, Hall et al., 1995, Lin and Dunson, 1993, Blust et al., 1992). In all these studies the apparent toxicity of cadmium was lowered as salinity was increased due to increased formation of CdC1+ and CDCl2 aqueous complexes that are non-toxic or of much lower toxicity than the free Cd2+ ion. Changes in pH exert both a biological and chemical effect on metal ion toxicity (Campbell and Stokes, 1985). Low pH favors greater metal ion solubility, and, in the absence of complexing ions, reduced speciation of the metal ion, which tends to increase toxicity compared to higher pH. However, Iow pH also enhances competition between H+ and metal ion for cell surface binding sites, which tends to decrease metal ion toxicity.

Carbonate system and nutrients in the Pearl River estuary, China: Seasonal and inter-annual variations

NASA Astrophysics Data System (ADS)

Guo, X.

2017-12-01

Located in southern China and surrounded by several metropolis, the Pearl River estuary is a large subtropical estuary under significant human perturbation. We examined the impact of sewage treatment rate on the water environmental factors. Carbonate system parameters (Dissolved inorganic carbon or DIC, Total alkalinity or TA, and pH), and nutrients were surveyed in the Pearl River estuary from 2000 to 2015. Spatially, concentrations of nutrients were high at low salinity and decreased with salinity in both wet and dry seasons although seasonal variation occurred. However, distribution patterns of DIC and TA differed in wet and dry seasons. In wet season, both DIC and TA were low at low salinity (600-1500 umol kg-1) and increased with salinity, but in dry season they were high at low salinity (3000-3500 umol kg-1) and decreased with salinity. Compared with the years before 2010, both values and distribution patterns of DIC, TA and pH were similar among the years in wet season, but they were conspicuously different in the upper estuary in dry season. Both DIC and TA were more than 1000 umol kg-1 lower than those in the years before 2010. For nutrients at low salinity, the ammonia concentration was much lower in the years after 2010 (200 vs. 400 umol kg-1 in wet season and 400 vs. 800 umol kg-1 in dry season), but nitrate concentration was slightly higher (180 vs 120 mmol kg-1 in wet season and 200 vs 180 mmol kg-1 in dry season). As a reference, carbonate system parameters and nutrients were stable among the 16 years in the adjacent northern South China Sea. The variations in biogeochemical processes induced by nutrients concentration and structure as a result of sewage discharge will be discussed in detail. The decrease in DIC, TA and nutrients in the upper Pearl River estuary after 2010 was due mainly to the improvement of sewage treatment rate and capacity.

Identification of differentially expressed genes in flax (Linum usitatissimum L.) under saline-alkaline stress by digital gene expression.

PubMed

Yu, Ying; Huang, Wengong; Chen, Hongyu; Wu, Guangwen; Yuan, Hongmei; Song, Xixia; Kang, Qinghua; Zhao, Dongsheng; Jiang, Weidong; Liu, Yan; Wu, Jianzhong; Cheng, Lili; Yao, Yubo; Guan, Fengzhi

2014-10-01

The salinization and alkalization of soil are widespread environmental problems, and alkaline salt stress is more destructive than neutral salt stress. Therefore, understanding the mechanism of plant tolerance to saline-alkaline stress has become a major challenge. However, little attention has been paid to the mechanism of plant alkaline salt tolerance. In this study, gene expression profiling of flax was analyzed under alkaline-salt stress (AS2), neutral salt stress (NSS) and alkaline stress (AS) by digital gene expression. Three-week-old flax seedlings were placed in 25 mM Na2CO3 (pH11.6) (AS2), 50mM NaCl (NSS) and NaOH (pH11.6) (AS) for 18 h. There were 7736, 1566 and 454 differentially expressed genes in AS2, NSS and AS compared to CK, respectively. The GO category gene enrichment analysis revealed that photosynthesis was particularly affected in AS2, carbohydrate metabolism was particularly affected in NSS, and the response to biotic stimulus was particularly affected in AS. We also analyzed the expression pattern of five categories of genes including transcription factors, signaling transduction proteins, phytohormones, reactive oxygen species proteins and transporters under these three stresses. Some key regulatory gene families involved in abiotic stress, such as WRKY, MAPKKK, ABA, PrxR and ion channels, were differentially expressed. Compared with NSS and AS, AS2 triggered more differentially expressed genes and special pathways, indicating that the mechanism of AS2 was more complex than NSS and AS. To the best of our knowledge, this was the first transcriptome analysis of flax in response to saline-alkaline stress. These data indicate that common and diverse features of saline-alkaline stress provide novel insights into the molecular mechanisms of plant saline-alkaline tolerance and offer a number of candidate genes as potential markers of tolerance to saline-alkaline stress. Copyright © 2014 Elsevier B.V. All rights reserved.

Survey of fishes and environmental conditions in Abbotts Lagoon, Point Reyes National Seashore, California

USGS Publications Warehouse

Saiki, M.K.; Martin, B.A.

2001-01-01

This study was conducted to gain a better understanding of fishery resources in Abbotts Lagoon, Point Reyes National Seashore. During February/March, May, August, and November 1999, fish were sampled with floating variable-mesh gill nets and small minnow traps from as many as 14 sites in the lagoon. Water temperature, dissolved oxygen, pH, total ammonia(NH3 + NH4+), salinity, turbidity, water depth, and bottom substrate composition were also measured at each site. A total of 2,656 fish represented by eight species was captured during the study. Gill nets captured Sacramento perch, Archoplites interruptus; largemouth bass, Micropterus salmoides; Pacific herring, Clupea pallasi; prickly sculpin, Cottus asper, silver surfperch, Hyperprosopon ellipticum; longfin smelt, Spirinchus thaleichthys; and striped bass, Morone saxatilis; whereas minnow traps captured Sacramento perch; prickly sculpin; and threespine stickleback, Gasterosteus aculeatus. Cluster analysis (Ward's minimum variance method of fish catch statistics identified two major species assemblages-the first dominated by Sacramento perch and, to a lesser extent, by largemouth bass, and the second dominated by Pacific herring and threespine stickleback. Simple discriminant analysis of environmental variables indicated that salinity contributed the most towards separating the two assemblages.