Design and Operation of a Fast, Thin-Film Thermocouple Probe on a Turbine Engine

NASA Technical Reports Server (NTRS)

Meredith, Roger D.; Wrbanek, John D.; Fralick, Gustave C.; Greer, Lawrence C., III; Hunter, Gary W.; Chen, Liang-Yu

2014-01-01

As a demonstration of technology maturation, a thin-film temperature sensor probe was fabricated and installed on a F117 turbofan engine via a borescope access port to monitor the temperature experienced in the bleed air passage of the compressor area during an engine checkout test run. To withstand the harsh conditions experienced in this environment, the sensor probe was built from high temperature materials. The thin-film thermocouple sensing elements were deposited by physical vapor deposition using pure metal elements, thus avoiding the inconsistencies of sputter-depositing particular percentages of materials to form standardized alloys commonly found in thermocouples. The sensor probe and assembly were subjected to a strict protocol of multi-axis vibrational testing as well as elevated temperature pressure testing to be qualified for this application. The thin-film thermocouple probe demonstrated a faster response than a traditional embedded thermocouple during the engine checkout run.

Piezo-thermal Probe Array for High Throughput Applications

PubMed Central

Gaitas, Angelo; French, Paddy

2012-01-01

Microcantilevers are used in a number of applications including atomic-force microscopy (AFM). In this work, deflection-sensing elements along with heating elements are integrated onto micromachined cantilever arrays to increase sensitivity, and reduce complexity and cost. An array of probes with 5–10 nm gold ultrathin film sensors on silicon substrates for high throughput scanning probe microscopy is developed. The deflection sensitivity is 0.2 ppm/nm. Plots of the change in resistance of the sensing element with displacement are used to calibrate the probes and determine probe contact with the substrate. Topographical scans demonstrate high throughput and nanometer resolution. The heating elements are calibrated and the thermal coefficient of resistance (TCR) is 655 ppm/K. The melting temperature of a material is measured by locally heating the material with the heating element of the cantilever while monitoring the bending with the deflection sensing element. The melting point value measured with this method is in close agreement with the reported value in literature. PMID:23641125

Thermistor holder for skin-temperature measurements

NASA Technical Reports Server (NTRS)

Greenleaf, J. E.; Williams, B. A.

1974-01-01

Sensing head of thermistor probe is supported in center area of plastic ring which has tabs so that it can be anchored in place by rubber bands or adhesive tapes. Device attaches probes to human subjects practically, reliably, and without affecting characteristics of skin segment being measured.

A Two-Dimensional Flow Sensor with Integrated Micro Thermal Sensing Elements and a Back Propagation Neural Network

PubMed Central

Que, Ruiyi; Zhu, Rong

2014-01-01

This paper demonstrates a novel flow sensor with two-dimensional 360° direction sensitivity achieved with a simple structure and a novel data fusion algorithm. Four sensing elements with roundabout wires distributed in four quadrants of a circle compose the sensor probe, and work in constant temperature difference (CTD) mode as both Joule heaters and temperature detectors. The magnitude and direction of a fluid flow are measured by detecting flow-induced temperature differences among the four elements. The probe is made of Ti/Au thin-film with a diameter of 2 mm, and is fabricated using micromachining techniques. When a flow goes through the sensor, the flow-induced temperature differences are detected by the sensing elements that also serve as the heaters of the sensor. By measuring the temperature differences among the four sensing elements symmetrically distributed in the sensing area, a full 360° direction sensitivity can be obtained. By using a BP neural network to model the relationship between the readouts of the four sensor elements and flow parameters and execute data fusion, the magnitude and direction of the flow can be deduced. Validity of the sensor design was proven through both simulations and experiments. Wind tunnel experimental results show that the measurement accuracy of the airflow speed reaches 0.72 m/s in the range of 3 m/s–30 m/s and the measurement accuracy of flow direction angle reaches 1.9° in the range of 360°. PMID:24385032

A two-dimensional flow sensor with integrated micro thermal sensing elements and a back propagation neural network.

PubMed

Que, Ruiyi; Zhu, Rong

2013-12-31

This paper demonstrates a novel flow sensor with two-dimensional 360° direction sensitivity achieved with a simple structure and a novel data fusion algorithm. Four sensing elements with roundabout wires distributed in four quadrants of a circle compose the sensor probe, and work in constant temperature difference (CTD) mode as both Joule heaters and temperature detectors. The magnitude and direction of a fluid flow are measured by detecting flow-induced temperature differences among the four elements. The probe is made of Ti/Au thin-film with a diameter of 2 mm, and is fabricated using micromachining techniques. When a flow goes through the sensor, the flow-induced temperature differences are detected by the sensing elements that also serve as the heaters of the sensor. By measuring the temperature differences among the four sensing elements symmetrically distributed in the sensing area, a full 360° direction sensitivity can be obtained. By using a BP neural network to model the relationship between the readouts of the four sensor elements and flow parameters and execute data fusion, the magnitude and direction of the flow can be deduced. Validity of the sensor design was proven through both simulations and experiments. Wind tunnel experimental results show that the measurement accuracy of the airflow speed reaches 0.72 m/s in the range of 3 m/s-30 m/s and the measurement accuracy of flow direction angle reaches 1.9° in the range of 360°.

EXTASE - An Experimental Thermal Probe For Applications In Snow Research And Earth Sciences

NASA Astrophysics Data System (ADS)

Schröer, K.; Seiferlin, K.; Marczewski, W.; Spohn, T.

EXTASE is a spin-off project from the Rosetta Lander (MUPUS) thermal probe, both funded by DLR. The application of this probe is to be tested in different fields e.g. in snow research, agriculture, permafrost etc. The probe penetrates the surface ca. 32 cm and provides a temperature profile (16 sensors) and thermal conductivity profile of the penetrated layer. The main advantages of the probe in comparison to common temperature profile measurement methods are: -no need to excavate material -minimized influence of the probe on the temperature field -minimized modification of the microstructure of the studied medium. Presently we are concentrating on agriculture (soil humidity) and snow research. Fur- ther applications could be: monitoring waste deposits and the heat set free by decom- position, volcanology and ground truth for remote sensing. We present the general concept of the probe, some temperature profiles measured during a field measurement campaign to demonstrate the capability of this new technique and first experiments made in the laboratory. First attempts to calculate thermal diffusivity and conductivity from the data are also given.

Brady's Geothermal Field - DTS Raw Data

DOE Data Explorer

Thomas Coleman

2016-03-26

The submitted data correspond to the complete raw temperature datasets captured by the distributed temperature sensing (DTS) horizontal and vertical arrays during the PoroTomo Experiment. Files in each submitted resource include: .xml (level 0): Data that includes Stokes, Anti-Stokes, and Temperature data .csv (level 1): Data that includes temperature PT100: Reference probe data

Method for determining the effects of oxygen plasma on a specimen

NASA Technical Reports Server (NTRS)

Whitaker, Ann F. (Inventor)

1991-01-01

A method for determining the effects of exposure of oxygen plasma on a specimen such as a thin film polymer or thin metals. The method includes providing an apparatus with a chamber having a holder supporting the polymer specimen in a plasma environment provided in the chamber. The chamber is regulated to a predetermined pressure and set temperature prior to the introduction of oxygen plasma therein. The specimen is then subjected to the plasma environment for a predetermined time during which time the temperature of the specimen is sensed and regulated to be maintained at the set temperature. Temperature sensing is accomplished by a probe which senses any changes in bulk sample temperature. Temperature regulation is provided by a thermoelectric module and by a coolant flow tube.

EXTASE - An Experimental Thermal Probe for Applications in Snow Research and Earth Sciences

NASA Astrophysics Data System (ADS)

Schroeer, K.; Seiferlin, K.; Marczewski, W.; Gadomski, S.; Spohn, T.

2002-12-01

EXTASE is a spin-off project from the Rosetta Lander (MUPUS) thermal probe, funded by DLR. The application of this probe is to be tested in different fields, e.g. in snow research, agriculture, permafrost etc. The system consists of the probe itself with a portable field electronic and a computer for control of the system and storage of the data. The probe penetrates the surface ca. 32 cm deep and provides a temperature profile (16 sensors) and thermal conductivity profile of the penetrated layer. The main advantages of the probe in comparison to common temperature profile measurement methods are: - no need to excavate material - minimized influence of the probe on the temperature field - minimized modification of the microstructure of the studied medium. Presently we are concentrating on agriculture (soil humidity) and snow research. Further applications could be e.g.: monitoring waste deposits and the heat released by decomposition, volcanology and ground truth for remote sensing. We present the general concept of the probe and also data obtained during different field measurement campaigns with prototypes of the probe.

Fiber-Optic Surface Temperature Sensor Based on Modal Interference.

PubMed

Musin, Frédéric; Mégret, Patrice; Wuilpart, Marc

2016-07-28

Spatially-integrated surface temperature sensing is highly useful when it comes to controlling processes, detecting hazardous conditions or monitoring the health and safety of equipment and people. Fiber-optic sensing based on modal interference has shown great sensitivity to temperature variation, by means of cost-effective image-processing of few-mode interference patterns. New developments in the field of sensor configuration, as described in this paper, include an innovative cooling and heating phase discrimination functionality and more precise measurements, based entirely on the image processing of interference patterns. The proposed technique was applied to the measurement of the integrated surface temperature of a hollow cylinder and compared with a conventional measurement system, consisting of an infrared camera and precision temperature probe. As a result, the optical technique is in line with the reference system. Compared with conventional surface temperature probes, the optical technique has the following advantages: low heat capacity temperature measurement errors, easier spatial deployment, and replacement of multiple angle infrared camera shooting and the continuous monitoring of surfaces that are not visually accessible.

Flexible Cryogenic Temperature and Liquid-Level Probes

NASA Technical Reports Server (NTRS)

Haberbusch, Mark

2003-01-01

Lightweight, flexible probes have been developed for measuring temperatures at multiple locations in tanks that contain possibly pressurized cryogenic fluids. If the fluid in a given tank is subcritical (that is, if it consists of a liquid and its vapor), then in one of two modes of operation, the temperature measurements made by a probe of this type can be used to deduce the approximate level of the liquid. The temperature sensors are silicon diodes located at intervals along a probe. If the probe is to be used to measure a temperature gradient along a given axis in the tank, then the probe must be mounted along that axis. In the non-liquid-level-sensing temperature-measurement mode, a constant small electric current is applied to each diode and the voltage across the diode . a known function of the current and temperature . is measured as an indication of its temperature. For the purpose of this measurement, "small electric current" signifies a current that is not large enough to cause a significant increase in the measured temperature. More specifically, the probe design calls for a current of 10 A, which, in the cryogenic temperature range of interest, generates heat at a rate of only about 0.01 mW per diode. In the liquid-level-sensing mode, one applies a larger current (30 mA) to each diode so as to heat each diode appreciably (with a power of about 36 mW in the temperature range of interest). Because the liquid cools the diode faster than does the vapor, the temperature of the diode is less when diode is immersed in the liquid than when it is above the surface of the liquid. Thus, the temperature (voltage) reading from each diode can be used to determine whether the liquid level is above or below the diode, and one can deduce that the liquid level lies between two adjacent diodes, the lower one of which reads a significantly lower temperature. The aforementioned techniques for measuring temperature and deducing liquid level are not new. What is new here are the designs of the probes and of associated external electronic circuitry. In each probe, the diodes and the lead wires are embedded in a strong, lightweight, flexible polyimide strip. Each probe is constructed as an integral unit that includes a multipin input/output plug or socket for solderless connection of the lead wires to the external circuitry. The polyimide strip includes mounting tabs with holes that can accommodate rivets, screws, or other fasteners. Alternatively, a probe can be mounted by use of an epoxy. A probe can be manufactured to almost any length or width, and the diodes can be embedded at almost any desired locations along and across the polyimide strip. In designing a probe for a specific application, one seeks a compromise between (1) minimizing the number of diodes in order to minimize the complexity of input/output connections and external electronic circuitry while (2) using enough diodes to obtain the required precision. Optionally, to minimize spurious heating of the cryogenic fluid, the external circuitry can be designed to apply power to the probe only during brief measurement intervals. Assuming that the external circuitry is maintained at a steady temperature, a power-on interval of only a few seconds is sufficient to obtain accurate data on temperatures and/or the height of the liquid/vapor interface.

Measurement of Turbulent Pressure and Temperature Fluctuations in a Gas Turbine Combustor

NASA Technical Reports Server (NTRS)

Povinelli, Louis (Technical Monitor); LaGraff, John E.; Bramanti, Cristina; Pldfield, Martin; Passaro, Andrea; Biagioni, Leonardo

2004-01-01

The report summarizes the results of the redesign efforts directed towards the gas-turbine combustor rapid-injector flow diagnostic probe developed under sponsorship of NASA-GRC and earlier reported in NASA-CR-2003-212540. Lessons learned during the theoretical development, developmental testing and field-testing in the previous phase of this research were applied to redesign of both the probe sensing elements and of the rapid injection device. This redesigned probe (referred to herein as Turboprobe) has been fabricated and is ready, along with the new rapid injector, for field-testing. The probe is now designed to capture both time-resolved and mean total temperatures, total pressures and, indirectly, one component of turbulent fluctuations.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nabeel A. Riza

The goals of the this part of the Continuation Phase 2 period (Oct. 1, 06 to March 31, 07) of this project were to (a) fabricate laser-doped SiC wafers and start testing the SiC chips for individual gas species sensing under high temperature and pressure conditions and (b) demonstrate the designs and workings of a temperature probe suited for industrial power generation turbine environment. A focus of the reported work done via Kar UCF LAMP lab. is to fabricate the embedded optical phase or doped microstructures based SiC chips, namely, Chromium (C), Boron (B) and Aluminum (Al) doped 4H-SiC, andmore » to eventually deploy such laser-doped chips to enable gas species sensing under high temperature and pressure. Experimental data is provided from SiC chip optical response for various gas species such as pure N2 and mixtures of N2 and H{sub 2}, N{sub 2} and CO, N{sub 2} and CO{sub 2}, and N{sub 2} and CH{sub 4}. Another main focus of the reported work was a temperature sensor probe assembly design and initial testing. The probe transmit-receive fiber optics were designed and tested for electrically controlled alignment. This probe design was provided to overcome mechanical vibrations in typical industrial scenarios. All these goals have been achieved and are described in detail in the report.« less

Eddy Current Sensing of Torque in Rotating Shafts

NASA Astrophysics Data System (ADS)

Varonis, Orestes J.; Ida, Nathan

2013-12-01

The noncontact torque sensing in machine shafts is addressed based on the stress induced in a press-fitted magnetoelastic sleeve on the shaft and eddy current sensing of the changes of electrical conductivity and magnetic permeability due to the presence of stress. The eddy current probe uses dual drive, dual sensing coils whose purpose is increased sensitivity to torque and decreased sensitivity to variations in distance between probe and shaft (liftoff). A mechanism of keeping the distance constant is also employed. Both the probe and the magnetoelastic sleeve are evaluated for performance using a standard eddy current instrument. An eddy current instrument is also used to drive the coils and analyze the torque data. The method and sensor described are general and adaptable to a variety of applications. The sensor is suitable for static and rotating shafts, is independent of shaft diameter and operational over a large range of torques. The torque sensor uses a differential eddy current measurement resulting in cancellation of common mode effects including temperature and vibrations.

Acoustical Measurement Of Furnace Temperatures

NASA Technical Reports Server (NTRS)

Parthasarathy, Shakkottai; Venkateshan, Shakkottai P.

1989-01-01

Simple probes withstand severe conditions, yet give spatially-resolved temperature readings. Prototype acoustical system developed to measure temperatures from ambient to 1,800 degree F in such structures as large industrial lime kilns and recovery-boiler furnaces. Pulses of sound reflected from obstructions in sensing tube. Speed of sound and temperature in each segment deduced from travel times of pulses.

SSTAC/ARTS review of the draft Integrated Technology Plan (ITP). Volume 8: Aerothermodynamics Automation and Robotics (A/R) systems sensors, high-temperature superconductivity

NASA Technical Reports Server (NTRS)

1991-01-01

Viewgraphs of briefings presented at the SSTAC/ARTS review of the draft Integrated Technology Plan (ITP) on aerothermodynamics, automation and robotics systems, sensors, and high-temperature superconductivity are included. Topics covered include: aerothermodynamics; aerobraking; aeroassist flight experiment; entry technology for probes and penetrators; automation and robotics; artificial intelligence; NASA telerobotics program; planetary rover program; science sensor technology; direct detector; submillimeter sensors; laser sensors; passive microwave sensing; active microwave sensing; sensor electronics; sensor optics; coolers and cryogenics; and high temperature superconductivity.

SSTAC/ARTS review of the draft Integrated Technology Plan (ITP). Volume 8: Aerothermodynamics Automation and Robotics (A/R) systems sensors, high-temperature superconductivity

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

Viewgraphs of briefings presented at the SSTAC/ARTS review of the draft Integrated Technology Plan (ITP) on aerothermodynamics, automation and robotics systems, sensors, and high-temperature superconductivity are included. Topics covered include: aerothermodynamics; aerobraking; aeroassist flight experiment; entry technology for probes and penetrators; automation and robotics; artificial intelligence; NASA telerobotics program; planetary rover program; science sensor technology; direct detector; submillimeter sensors; laser sensors; passive microwave sensing; active microwave sensing; sensor electronics; sensor optics; coolers and cryogenics; and high temperature superconductivity.

Remote Sensing of the Solar Wind Density, Speed, and Temperature in the Region between the Sun and Parker Solar Probe

NASA Astrophysics Data System (ADS)

Davila, J. M.; Reginald, N. L.

2017-12-01

A coronagraph is the tool of choice to understand and observe the structure of the corona from space. The novel coronagraph concept presented her provides a new scientific capability that will allow the measurement of density, temperature, and flow velocity in the solar atmosphere. This instrument will provide the first remote sensing measurement of the global solar wind temperature, density, and flow speed in the regions between 3 and 8 Rsun. It is in this region that the manority of the solar wind acceleration takes place, and where the ion compsition of the solar wind is "frozen in". This is also the region of the corona that links the surface of the Sun to the Parker Solar Probe and to Solar Orbiter. The observations suggested here would dramatically improve our understanding of solar wind formation and evolution in this critical region.

Principles and Applications of the qPlus Sensor

NASA Astrophysics Data System (ADS)

Giessibl, Franz J.

The concept of the atomic force microscope (AFM) is a very simple one: map the surface of a sample by a sharp probe that scans over the surface similar to the finger of a blind person that reads Braille characters. In AFM, the role of that finger is taken by the probe tip that senses the presence of the sample surface by detecting the force between the tip of the probe and a sample. The qPlus sensor is a self sensing cantilever based on a quartz tuning fork that supplements the traditional microfabricated cantilevers made of silicon. Quartz tuning forks are used in the watch industry in quantities of billions annually, with the positive effects on quality and perfection. Three properties of these quartz-based sensors simplify the AFM significantly: (1) the piezoelectricity of quartz allows simple self sensing, (2) the mechanical properties of quartz show very small variations with temperature, and (3) the given stiffness of many quartz tuning forks is close to the ideal stiffness of cantilevers. The key properties of the qPlus sensor are a large stiffness that allows small amplitude operation, the large size that allows to mount single-crystal probe tips, and the self-sensing piezoelectric detection mechanism.

Recent Advances of Activatable Molecular Probes Based on Semiconducting Polymer Nanoparticles in Sensing and Imaging

PubMed Central

Lyu, Yan

2017-01-01

Molecular probes that change their signals in response to the target of interest have a critical role in fundamental biology and medicine. Semiconducting polymer nanoparticles (SPNs) have recently emerged as a new generation of purely organic photonic nanoagents with desirable properties for biological applications. In particular, tunable optical properties of SPNs allow them to be developed into photoluminescence, chemiluminescence, and photoacoustic probes, wherein SPNs usually serve as the energy donor and internal reference for luminescence and photoacoustic probes, respectively. Moreover, facile surface modification and intraparticle engineering provide the versatility to make them responsive to various biologically and pathologically important substances and indexes including small‐molecule mediators, proteins, pH and temperature. This article focuses on recent advances in the development of SPN‐based activatable molecular probes for sensing and imaging. The designs and applications of these probes are discussed in details, and the present challenges to further advance them into life science are also analyzed. PMID:28638783

In-place recalibration technique applied to a capacitance-type system for measuring rotor blade tip clearance

NASA Technical Reports Server (NTRS)

Barranger, J. P.

1978-01-01

The rotor blade tip clearance measurement system consists of a capacitance sensing probe with self contained tuning elements, a connecting coaxial cable, and remotely located electronics. Tests show that the accuracy of the system suffers from a strong dependence on probe tip temperature and humidity. A novel inplace recalibration technique was presented which partly overcomes this problem through a simple modification of the electronics that permits a scale factor correction. This technique, when applied to a commercial system significantly reduced errors under varying conditions of humidity and temperature. Equations were also found that characterize the important cable and probe design quantities.

Identification of a dynamic temperature threshold for soil moisture freeze/thaw (F/T) state classification using soil real dielectric constant derivatives.

NASA Astrophysics Data System (ADS)

Pardo, R.; Berg, A. A.; Warland, J. S.

2017-12-01

The use of microwave remote sensing for surface ground ice detection has been well documented using both active and passive systems. Typical validation of these remotely sensed F/T state products relies on in-situ air or soil temperature measurements and a threshold of 0°C to identify frozen soil. However, in soil pores, the effects of capillary and adsorptive forces combine with the presence of dissolved salts to depress the freezing point. This is further confounded by the fact that water over this temperature range releases/absorbs latent heat of freezing/fusion. Indeed, recent results from SLAPEx2015, a campaign conducted to evaluate the ability to detect F/T state and examine the controls on F/T detection at multiple resolutions, suggest that using a soil temperature of 0°C as a threshold for freezing may not be appropriate. Coaxial impedance sensors, like Steven's HydraProbeII (HP), are the most widely used soil sensor in water supply forecast and climatological networks. These soil moisture probes have recently been used to validate remote sensing F/T products. This kind of validation is still relatively uncommon and dependent on categorical techniques based on seasonal reference states of frozen and non-frozen soil conditions. An experiment was conducted to identify the correlation between the phase state of the soil moisture and the probe measurements. Eight soil cores were subjected to F/T transitions in an environmental chamber. For each core, at a depth of 2.5 cm, the temperature and real dielectric constant (rdc) were measured every five minutes using HPs while two heat pulse probes captured the apparent heat capacity 24 minutes apart. Preliminary results show the phase transition of water is bounded by inflection points in the soil temperature, attributed to latent heat. The rdc, however, appears to be highly sensitive to changes in the water preceding the phase change. This opens the possibility of estimating a dynamic temperature threshold for soil F/T by identifying the soil temperatures at the times during which these inflection points in the soil rdc occur. This technique provides a more accurate threshold for F/T product than the static reference temperature currently established.

One-step synthesis of multi-emission carbon nanodots for ratiometric temperature sensing

NASA Astrophysics Data System (ADS)

Nguyen, Vanthan; Yan, Lihe; Xu, Huanhuan; Yue, Mengmeng

2018-01-01

Measuring temperature with greater precision at localized small length scales or in a nonperturbative manner is a necessity in widespread applications, such as integrated photonic devices, micro/nano electronics, biology, and medical diagnostics. To this context, use of nanoscale fluorescent temperature probes is regarded as the most promising method for temperature sensing because they are noninvasive, accurate, and enable remote micro/nanoscale imaging. Here, we propose a novel ratiometric fluorescent sensor for nanothermometry using carbon nanodots (C-dots). The C-dots were synthesized by one-step method using femtosecond laser ablation and exhibit unique multi-emission property due to emissions from abundant functional groups on its surface. The as-prepared C-dots demonstrate excellent ratiometric temperature sensing under single wavelength excitation that achieves high temperature sensitivity with a 1.48% change per °C ratiometric response over wide-ranging temperature (5-85 °C) in aqueous buffer. The ratiometric sensor shows excellent reversibility and stability, holding great promise for the accurate measurement of temperature in many practical applications.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yang, Haw; Hsia, Chih-Hao

Novel Mn.sup.2+-doped quantum dots are provided. These Mn.sup.2+-doped quantum dots exhibit excellent temperature sensitivity in both organic solvents and water-based solutions. Methods of preparing the Mn.sup.2+-doped quantum dots are provided. The Mn.sup.2+-doped quantum dots may be prepared via a stepwise procedure using air-stable and inexpensive chemicals. The use of air-stable chemicals can significantly reduce the cost of synthesis, chemical storage, and the risk associated with handling flammable chemicals. Methods of temperature sensing using Mn.sup.2+-doped quantum dots are provided. The stepwise procedure provides the ability to tune the temperature-sensing properties to satisfy specific needs for temperature sensing applications. Water solubility maymore » be achieved by passivating the Mn.sup.2+-doped quantum dots, allowing the Mn.sup.2+-doped quantum dots to probe the fluctuations of local temperature in biological environments.« less

Diode-Laser Absorption Sensor for Line-of-Sight Gas Temperature Distributions

NASA Astrophysics Data System (ADS)

Sanders, Scott T.; Wang, Jian; Jeffries, Jay B.; Hanson, Ronald K.

2001-08-01

Line-of-sight diode-laser absorption techniques have been extended to enable temperature measurements in nonuniform-property flows. The sensing strategy for such flows exploits the broad wavelength-scanning abilities ( >1.7 nm ~ 30 cm-1 ) of a vertical cavity surface-emitting laser (VCSEL) to interrogate multiple absorption transitions along a single line of sight. To demonstrate the strategy, a VCSEL-based sensor for oxygen gas temperature distributions was developed. A VCSEL beam was directed through paths containing atmospheric-pressure air with known (and relatively simple) temperature distributions in the 200 -700 K range. The VCSEL was scanned over ten transitions in the R branch of the oxygen A band near 760 nm and optionally over six transitions in the P branch. Temperature distribution information can be inferred from these scans because the line strength of each probed transition has a unique temperature dependence; the measurement accuracy and resolution depend on the details of this temperature dependence and on the total number of lines scanned. The performance of the sensing strategy can be optimized and predicted theoretically. Because the sensor exhibits a fast time response ( ~30 ms) and can be adapted to probe a variety of species over a range of temperatures and pressures, it shows promise for industrial application.

Tapered optical fiber tip probes based on focused ion beam-milled Fabry-Perot microcavities

NASA Astrophysics Data System (ADS)

André, Ricardo M.; Warren-Smith, Stephen C.; Becker, Martin; Dellith, Jan; Rothhardt, Manfred; Zibaii, M. I.; Latifi, H.; Marques, Manuel B.; Bartelt, Hartmut; Frazão, Orlando

2016-09-01

Focused ion beam technology is combined with dynamic chemical etching to create microcavities in tapered optical fiber tips, resulting in fiber probes for temperature and refractive index sensing. Dynamic chemical etching uses hydrofluoric acid and a syringe pump to etch standard optical fibers into cone structures called tapered fiber tips where the length, shape, and cone angle can be precisely controlled. On these tips, focused ion beam is used to mill several different types of Fabry-Perot microcavities. Two main cavity types are initially compared and then combined to form a third, complex cavity structure. In the first case, a gap is milled on the tapered fiber tip which allows the external medium to penetrate the light guiding region and thus presents sensitivity to external refractive index changes. In the second, two slots that function as mirrors are milled on the tip creating a silica cavity that is only sensitive to temperature changes. Finally, both cavities are combined on a single tapered fiber tip, resulting in a multi-cavity structure capable of discriminating between temperature and refractive index variations. This dual characterization is performed with the aid of a fast Fourier transform method to separate the contributions of each cavity and thus of temperature and refractive index. Ultimately, a tapered optical fiber tip probe with sub-standard dimensions containing a multi-cavity structure is projected, fabricated, characterized and applied as a sensing element for simultaneous temperature and refractive index discrimination.

Dynamic assessment of women pelvic floor function by using a fiber Bragg grating sensor system

NASA Astrophysics Data System (ADS)

Ferreira, Luis A.; Araújo, Francisco M.; Mascarenhas, Teresa; Natal Jorge, Renato M.; Fernandes, António A.

2006-02-01

We present a novel sensing system consisting of an intravaginal probe and an optoelectronic measurement unit, which allows an easy, comfortable and quantitative dynamic evaluation of women pelvic floor muscle strength. The sensing probe is based on a silicone cylinder that transduces radial muscle pressure into axial load applied to a fiber Bragg grating strain sensor. The performance of a first sensor probe prototype with temperature referentiation and of the autonomous, portable optoelectronic measurement unit with data logging capabilities and graphical user interface is disclosed. The presented results refer to an ongoing collaboration work between researchers from the Medical, Optoelectronics and Mechanical areas, directed to the development of equipment that can assist in medical practice and help in the research of primary mechanisms responsible for several pelvic floor disorders, in particular urogenital prolapses.

Heated Surface Temperatures Measured by Infrared Detector in a Cascade Environment

NASA Technical Reports Server (NTRS)

Boyle, Robert J.

2002-01-01

Investigators have used infrared devices to accurately measure heated surface temperatures. Several of these applications have been for turbine heat transfer studies involving film cooling and surface roughness, typically, these measurements use an infrared camera positioned externally to the test section. In cascade studies, where several blades are used to ensure periodic flow, adjacent blades block the externally positioned camera's views of the test blade. To obtain a more complete mapping of the surface temperatures, researchers at the NASA Glenn Research Center fabricated a probe with an infrared detector to sense the blade temperatures. The probe size was kept small to minimize the flow disturbance. By traversing and rotating the probe, using the same approach as for total pressure surveys, one can find the blade surface temperatures. Probe mounted infrared detectors are appropriate for measuring surface temperatures where an externally positioned infrared camera is unable to completely view the test object. This probe consists of a 8-mm gallium arsenide (GaAs) lens mounted in front of a mercury-cadmium-zinc-tellurium (HgCdZnTe) detector. This type of photovoltaic detector was chosen because of its high sensitivity to temperature when the detector is uncooled. The particular application is for relatively low surface temperatures, typically ambient to 100 C. This requires a detector sensitive at long wavelengths. The detector is a commercial product enclosed in a 9-mm-diameter package. The GaAs lens material was chosen because of its glass-like hardness and its good long-wavelength transmission characteristics. When assembled, the 6.4-mm probe stem is held in the traversing actuator. Since the entire probe is above the measurement plane, the flow field disturbance in the measurement plane is minimized. This particular probe body is somewhat wider than necessary, because it was designed to have replaceable detectors and lenses. The signal for the detector is fed through the hollow probe body. The detector's signal goes to an externally mounted preamplifier. The detector assembly, along with a preamplifier, is calibrated as a function of the surface temperature for various detector temperatures. The output voltage is a function of both the detector and object temperatures.

High bandwidth piezoresistive force probes with integrated thermal actuation

PubMed Central

Doll, Joseph C.; Pruitt, Beth L.

2012-01-01

We present high-speed force probes with on-chip actuation and sensing for the measurement of pN-scale forces at the microsecond time scale. We achieve a high resonant frequency in water (1–100 kHz) with requisite low spring constants (0.3–40 pN/nm) and low integrated force noise (1–100 pN) by targeting probe dimensions on the order of 300 nm thick, 1–2 μm wide and 30–200 μm long. Forces are measured using silicon piezoresistors while the probes are actuated thermally with an aluminum unimorph and silicon heater. The piezoresistive sensors are designed using open source numerical optimization code that incorporates constraints on operating temperature. Parylene passivation enables operation in ionic media and we demonstrate simultaneous actuation and sensing. The improved design and fabrication techniques that we describe enable a 10–20 fold improvement in force resolution or measurement bandwidth over prior piezoresistive cantilevers of comparable thickness. PMID:23175616

Temperature measurement based on photoluminescence of Er3+ doped Sr0.3Cd0.7F2 microcrystal coupled to scanning thermal microscopy

NASA Astrophysics Data System (ADS)

Trannoy, N.; Sayoud, A.; Diaf, M.; Duvaut, Th.; Jouart, J. P.; Grossel, Ph.

2015-04-01

Rare earth doped sub-micrometric luminescent materials are promising candidates for temperature sensing and play an efficient role in many technological fields. In this paper, a new optical sensor is developed for measuring local temperatures. This sensor is based on a thermal-resistive probe and on photoluminescence of a luminescent fluoride microcrystal. The final purpose is to develop a device calibrated in temperature and capable of acquiring images of local temperature at sub-micrometric scale. Indeed, the sensor temperature can be obtained in two distinct ways: one from the thermal probe parameters and the other from the green photoluminescence generated in the anti-Stokes mode by the active Er ions directly excited by a red laser. The thermal probe is based on Wollaston wire whose thermal-resistive element is in platinum/rhodium. Its temperature is estimated from the probe electrical characteristics and a modeling. A microcrystal of Sr0.3Cd0.7F2: Er3+(4%)-Yb3+(6%) of about 25 μm in diameter is glued at the probe extremity. This luminescent material has the particularity to give a green emission spectrum with intensities sensitive to small temperature variations. Using the fluorescence intensity ratio (FIR) technique, the crystal temperature is estimated from the intensity measurements at green wavelengths 522, 540 and 549 nm by taking advantage of particular optical properties due to the crystalline nature of Sr0.3Cd0.7F2: Er3+-Yb3+. The microcrystal temperature is then assessed as a function of electric current in the thermal probe by applying the Boltzmann's equations. The coupling of the scanning thermal microscope (SThM) with the photoluminescence probe reveals that the particle fluorescence signal is affected by the temperature rise of an electrical microsystem submitted to a Joule heating. The first results are presented and discussed.

An "Inefficient Fin" Non-Dimensional Parameter to Measure Gas Temperatures Efficiently

NASA Technical Reports Server (NTRS)

Lemieux, Patrick; Murray, William; Cooke, Terry; Gerhardt, James

2012-01-01

A gas containment vessel that is not in thermal equilibrium with the bulk gas can affect its temperature measurement. The physical nature of many gas dynamics experiments often makes the accurate measurement of temperature a challenge. The environment itself typically requires that the thermocouple be sheathed, both to protect the wires and hot junction of the instrument from their environment, and to provide a smooth, rigid surface for pressure sealing of the enclosure. However, that enclosure may also be much colder than the gas to be sensed, or vice-versa. Either way, the effect of such gradients is to potentially skew the temperature measurements themselves, since heat may then be conducted by the instrument. Thermocouple designers traditionally address this problem by insulating the sheath from the thermocouple leads and hot junction as much as possible. The thermocouple leads are typically packed in a ceramic powder inside the sheath, protecting them somewhat from temperature gradients along the sheath, but there is no effective mechanism to shield the sheath from the enclosure body itself. Standard practice dictates that thermocouples be used in installations that do not present large thermal gradients along the probe. If this conduction dominates heat transfer near the tip of the probe, then temperature measurements may be expected to be skewed. While the same problem may be experienced in the measurement of temperature at various points within a solid in a gradient, it tends to be aggravated in the measurements of gas temperature, since heat transfer dependent on convection is often less efficient than conduction along the thermocouple. The proposed solution is an inefficient fin thermocouple probe. Conventional wisdom suggests that in many experiments where gas flows through an enclosure (e.g., flow in pipe, manifold, nozzle, etc.), the thermocouple be introduced flush to the surface, so as not to interfere with the flow. In practice, however, many such experiments take place where the flow is already turbulent, so that a protruding thermocouple probe has a negligible effect on the flow characteristics. The key question then becomes just how far into the flow should a thermocouple protrude in order to properly sense the gas temperature at that point. Modeling the thermocouple as an "inefficient fin" directly addresses this question. The appropriate assumptions in this case are: one-dimensional conduction along the fin; steady-state, constant, and homogeneous thermal conductivity; negligible radiation; and a uniform, constant heat transfer coefficient over the probe surface. It is noted that the nature of the ceramic-filled probe makes the key assumption of homogeneous thermal conductivity that much more conservative.

Flight testing of a fiber optic temperature sensor

NASA Technical Reports Server (NTRS)

Finney, M. J.; Tregay, G. W.; Calabrese, P. R.

1993-01-01

A fiber optic temperature sensor (FOTS) system consisting of an optical probe, a flexible fiber optic cable, and an electro-optic signal processor was fabricated to measure the gas temperature in a turbine engine. The optical probe contained an emissive source embedded in a sapphire lightguide coupled to a fiber-optic jumper cable and was retrofitted into an existing thermocouple probe housing. The flexible fiber optic cable was constructed with 200 micron core, polyimide-coated fiber and was ruggedized for an aircraft environment. The electro-optic signal processing unit was used to ratio the intensities of two wavelength intervals and provided an analog output value of the indicated temperature. Subsequently, this optical sensor system was installed on a NASA Dryden F-15 Highly Integrated Digital Electronic Control (HIDEC) Aircraft Engine and several flight tests were conducted. Over the course of flight testing, the FOTS system's response was proportional to the average of the existing thermocouples sensing the changes in turbine engine thermal conditions.

A gold nanocluster-based fluorescent probe for simultaneous pH and temperature sensing and its application to cellular imaging and logic gates.

PubMed

Wu, Yun-Tse; Shanmugam, Chandirasekar; Tseng, Wei-Bin; Hiseh, Ming-Mu; Tseng, Wei-Lung

2016-06-07

Metal nanocluster-based nanomaterials for the simultaneous determination of temperature and pH variations in micro-environments are still a challenge. In this study, we develop a dual-emission fluorescent probe consisting of bovine serum albumin-stabilized gold nanoclusters (BSA-AuNCs) and fluorescein-5-isothiocyanate (FITC) as temperature- and pH-responsive fluorescence signals. Under single wavelength excitation the FITC/BSA-AuNCs exhibited well-separated dual emission bands at 525 and 670 nm. When FITC was used as a reference fluorophore, FITC/BSA-AuNCs showed a good linear response over the temperature range 1-71 °C and offered temperature-independent spectral shifts, temperature accuracy, activation energy, and reusability. The possible mechanism for high temperature-induced fluorescence quenching of FITC/BSA-AuNCs could be attributed to a weakening of the Au-S bond, thereby lowering the charge transfer from BSA to AuNCs. Additionally, the pH- and temperature-responsive properties of FITC/BSA-AuNCs allow simultaneous temperature sensing from 21 to 41 °C (at intervals of 5 °C) and pH from 6.0 to 8.0 (at intervals of 0.5 pH unit), facilitating the construction of two-input AND logic gates. Three-input AND logic gates were also designed using temperature, pH, and trypsin as inputs. The practicality of using FITC/BSA-AuNCs to determine the temperature and pH changes in HeLa cells is also validated.

Work function measurements of copper nanoparticle intercalated polyaniline nanocomposite thin films

NASA Astrophysics Data System (ADS)

Patil, U. V.; Ramgir, Niranjan S.; Bhogale, A.; Debnath, A. K.; Muthe, K. P.; Gadkari, S. C.; Kothari, D. C.

2017-05-01

The nature of contact between the electrode and the sensing material plays a crucial role in governing the sensing mechanism. Thin films of polyaniline (PANI) and copper-polyaniline nanocomposite (NC) have been deposited at room temperatures by in-situ oxidative polymerization of aniline in the presence of Cu nanoparticles. For sensing applications a thin film Au (gold) ˜100 nm is deposited and used as a conducting electrode. To understand the nature of contact (i.e., ohmic or Schottky) the work function of the conducting polyaniline and nanocomposite films were measured using Kelvin Probe method. I-V characteristics of PANI and NC films investigated at room temperatures further corroborates and confirms the formation of Ohmic contact as evident from work function measurements.

Carbon Nanotubes Blended Hydroxyapatite Ethanol Sensor

NASA Astrophysics Data System (ADS)

Anjum, S. R.; Khairnar, R. S.

2016-12-01

Nano crystals of Hydroxyapatite (HAp) were synthesized by a wet chemical precipitation method. The nano composite materials were developed by doping various weight concentrations of carbon nanotubes in HAp, followed by characterization using scanning electron microscopy, and X-ray diffraction. Thick films of these materials were prepared by using screen printing technique. The ethanol sensing properties of these nano crystals and nano composite films were investigated by two probe electrical method. The gas sensing features such as operating temperature, response and recovery time, maximum gas detection limit, etc. were studied, since these parameters are of prime importance for sensor. The results revealed that at room temperature, the composite materials exhibited improved sensing performance towards 100 ppm ethanol with fast response times. It also showed shorter recovery time with higher vapor uptake capacity. The ethanol adsorption processes on doped and undoped substrates can be explained by surface chemical reactions as well as providing the possible adsorption models. The novelty of this work lies in developing reusable sensor substrates for room temperature sensing.

Fluorescent probe for turn-on sensing of L-cysteine by ensemble of AuNCs and polymer protected AuNPs.

PubMed

Xu, Xiaozhe; Qiao, Juan; Li, Nan; Qi, Li; Zhang, Shufeng

2015-06-16

A new fluorescent probe based on ensemble of gold nanoclusters (AuNCs) and polymer protected gold nanoparticles (AuNPs) for turn-on sensing of L-cysteine was designed and prepared. The AuNCs were protected by bovine serum albumin and had strong fluorescence. The polymer protected AuNPs were synthesized by a facile in situ strategy at room temperature and could quench the fluorescence of AuNCs due to the Förster resonance energy transfer. Interestingly, it has been observed that the quenched fluorescence of AuNCs was recovered by L-cysteine, which could induce the aggregation of polymer protected AuNPs by sulfur group. Then the prepared fluorescent probe was successfully used for determination of L-Cys in human urines, which would have an evolving aspect and promote the subsequent exploration. Copyright © 2015 Elsevier B.V. All rights reserved.

A computerized Langmuir probe system

NASA Astrophysics Data System (ADS)

Pilling, L. S.; Bydder, E. L.; Carnegie, D. A.

2003-07-01

For low pressure plasmas it is important to record entire single or double Langmuir probe characteristics accurately. For plasmas with a depleted high energy tail, the accuracy of the recorded ion current plays a critical role in determining the electron temperature. Even for high density Maxwellian distributions, it is necessary to accurately model the ion current to obtain the correct electron density. Since the electron and ion current saturation values are, at best, orders of magnitude apart, a single current sensing resistor cannot provide the required resolution to accurately record these values. We present an automated, personal computer based data acquisition system for the determination of fundamental plasma properties in low pressure plasmas. The system is designed for single and double Langmuir probes, whose characteristics can be recorded over a bias voltage range of ±70 V with 12 bit resolution. The current flowing through the probes can be recorded within the range of 5 nA-100 mA. The use of a transimpedance amplifier for current sensing eliminates the requirement for traditional current sensing resistors and hence the need to correct the raw data. The large current recording range is realized through the use of a real time gain switching system in the negative feedback loop of the transimpedance amplifier.

Temperature-compensated distributed hydrostatic pressure sensor with a thin-diameter polarization-maintaining photonic crystal fiber based on Brillouin dynamic gratings.

PubMed

Teng, Lei; Zhang, Hongying; Dong, Yongkang; Zhou, Dengwang; Jiang, Taofei; Gao, Wei; Lu, Zhiwei; Chen, Liang; Bao, Xiaoyi

2016-09-15

A temperature-compensated distributed hydrostatic pressure sensor based on Brillouin dynamic gratings (BDGs) is proposed and demonstrated experimentally for the first time, to the best of our knowledge. The principle is to measure the hydrostatic pressure induced birefringence changes through exciting and probing the BDGs in a thin-diameter pure silica polarization-maintaining photonic crystal fiber. The temperature cross-talk to the hydrostatic pressure sensing can be compensated through measuring the temperature-induced Brillouin frequency shift (BFS) changes using Brillouin optical time-domain analysis. A distributed measurement of hydrostatic pressure is demonstrated experimentally using a 4-m sensing fiber, which has a high sensitivity, with a maximum measurement error less than 0.03 MPa at a 20-cm spatial resolution.

Investigation of remote sensing techniques of measuring soil moisture

NASA Technical Reports Server (NTRS)

Newton, R. W. (Principal Investigator); Blanchard, A. J.; Nieber, J. L.; Lascano, R.; Tsang, L.; Vanbavel, C. H. M.

1981-01-01

Major activities described include development and evaluation of theoretical models that describe both active and passive microwave sensing of soil moisture, the evaluation of these models for their applicability, the execution of a controlled field experiment during which passive microwave measurements were acquired to validate these models, and evaluation of previously acquired aircraft microwave measurements. The development of a root zone soil water and soil temperature profile model and the calibration and evaluation of gamma ray attenuation probes for measuring soil moisture profiles are considered. The analysis of spatial variability of soil information as related to remote sensing is discussed as well as the implementation of an instrumented field site for acquisition of soil moisture and meteorologic information for use in validating the soil water profile and soil temperature profile models.

Simultaneous measurement of magnetic field and temperature based on an etched TCFMI cascaded with an FBG

NASA Astrophysics Data System (ADS)

Yan, Guofeng; Zhang, Liang; He, Sailing

2016-04-01

In this paper, a dual-parameter measurement scheme based on an etched thin core fiber modal interferometer (TCMI) cascaded with a fiber Bragg grating (FBG) is proposed and experimentally demonstrated for simultaneous measurement of magnetic field and temperature. The magnetic field and temperature responses of the packaged TCFMI were first investigated, which showed that the magnetic field sensitivity could be highly enhanced by decreasing of the TCF diameter and the temperature-cross sensitivities were up to 3-7 Oe/°C at 1550 nm. Then, the theoretical analysis and experimental demonstration of the proposed dual-parameter sensing scheme were conducted. Experimental results show that, the reflection of the FBG has a magnetic field intensity and temperature sensitivities of -0.017 dB/Oe and 0.133 dB/°C, respectively, while the Bragg wavelength of the FBG is insensitive to magnetic field and has a temperature sensitivity of 13.23 pm/°C. Thus by using the sensing matrix method, the intensity of the magnetic field and the temperature variance can be measured, which enables magnetic field sensing under strict temperature environments. In the on-off time response test, the fabricated sensor exhibited high repeatability and short response time of ∼19.4 s. Meanwhile the reflective sensing probe type is more compact and practical for applications in hard-to-reach conditions.

Two Fiber Optical Fiber Thermometry

NASA Technical Reports Server (NTRS)

Jones, Mathew R.; Farmer, Jeffery T.; Breeding, Shawn P.

2000-01-01

An optical fiber thermometer consists of an optical fiber whose sensing tip is given a metallic coating. The sensing tip of the fiber is essentially an isothermal cavity, so the emission from this cavity will be approximately equal to the emission from a blackbody. Temperature readings are obtained by measuring the spectral radiative heat flux at the end of the fiber at two wavelengths. The ratio of these measurements and Planck's Law are used to infer the temperature at the sensing tip. Optical fiber thermometers have high accuracy, excellent long-term stability and are immune to electromagnetic interference. In addition, they can be operated for extended periods without requiring re-calibration. For these reasons. it is desirable to use optical fiber thermometers in environments such as the International Space Station. However, it has recently been shown that temperature readings are corrupted by emission from the fiber when extended portions of the probe are exposed to elevated temperatures. This paper will describe several ways in which the reading from a second fiber can be used to correct the corrupted temperature measurements. The accuracy and sensitivity to measurement uncertainty will be presented for each method.

Self-sensing cantilevers with integrated conductive coaxial tips for high-resolution electrical scanning probe metrology

DOE Office of Scientific and Technical Information (OSTI.GOV)

Haemmerli, Alexandre J.; Pruitt, Beth L., E-mail: pruitt@stanford.edu; Harjee, Nahid

The lateral resolution of many electrical scanning probe techniques is limited by the spatial extent of the electrostatic potential profiles produced by their probes. Conventional unshielded conductive atomic force microscopy probes produce broad potential profiles. Shielded probes could offer higher resolution and easier data interpretation in the study of nanostructures. Electrical scanning probe techniques require a method of locating structures of interest, often by mapping surface topography. As the samples studied with these techniques are often photosensitive, the typical laser measurement of cantilever deflection can excite the sample, causing undesirable changes electrical properties. In this work, we present the design,more » fabrication, and characterization of probes that integrate coaxial tips for spatially sharp potential profiles with piezoresistors for self-contained, electrical displacement sensing. With the apex 100 nm above the sample surface, the electrostatic potential profile produced by our coaxial tips is more than 2 times narrower than that of unshielded tips with no long tails. In a scan bandwidth of 1 Hz–10 kHz, our probes have a displacement resolution of 2.9 Å at 293 K and 79 Å at 2 K, where the low-temperature performance is limited by amplifier noise. We show scanning gate microscopy images of a quantum point contact obtained with our probes, highlighting the improvement to lateral resolution resulting from the coaxial tip.« less

Stark sublevels investigation in Y2WO6:Tm3+-Yb3+ phosphor for thermometry and internal temperature measurement

NASA Astrophysics Data System (ADS)

Soni, Abhishek Kumar

2018-05-01

Intensity ratio investigation in the Y2WO6:Tm3+-Yb3+ phosphors synthesized by solid state reaction method has been discussed first time under 980 nm laser diode excitation with the help of multiple peak fitting. The temperature dependent upconversion emission study has been performed for optical temperature sensing by using stark sublevels of 1G4 level of Tm3+ ion. The intensity of the two stark sublevels is varied due to the thermalization under the application of external temperature. The energy gap has been calculated about ∼427 cm‑1 of the two stark sublevels via Boltzmann’s population distribution law. The calculated sensitivity (maximum about ∼34 × 10‑4 K‑1 at 303 K) and optical heating properties prove the utility of the prepared phosphor in making optical temperature sensing probe and optical heater.

Exploring luminescence-based temperature sensing using protein-passivated gold nanoclusters.

PubMed

Chen, Xi; Essner, Jeremy B; Baker, Gary A

2014-08-21

We explore the analytical performance and limitations of optically monitoring aqueous-phase temperature using protein-protected gold nanoclusters (AuNCs). Although not reported elsewhere, we find that these bio-passivated AuNCs show pronounced hysteresis upon thermal cycling. This unwanted behaviour can be eliminated by several strategies, including sol-gel coating and thermal denaturation of the biomolecular template, introducing protein-templated AuNC probes as viable nanothermometers.

A thermal microprobe fabricated with wafer-stage processing

NASA Astrophysics Data System (ADS)

Zhang, Yongxia; Zhang, Yanwei; Blaser, Juliana; Sriram, T. S.; Enver, Ahsan; Marcus, R. B.

1998-05-01

A thermal microprobe has been designed and built for high resolution temperature sensing. The thermal sensor is a thin-film thermocouple junction at the tip of an atomic force microprobe (AFM) silicon probe needle. Only wafer-stage processing steps are used for the fabrication. For high resolution temperature sensing it is essential that the junction be confined to a short distance at the AFM tip. This confinement is achieved by a controlled photoresist coating process. Experiment prototypes have been made with an Au/Pd junction confined to within 0.5 μm of the tip, with the two metals separated elsewhere by a thin insulating oxide layer. Processing begins with double-polished, n-type, 4 in. diameter, 300-μm-thick silicon wafers. Atomically sharp probe tips are formed by a combination of dry and wet chemical etching, and oxidation sharpening. The metal layers are sputtering deposited and the cantilevers are released by a combination of KOH and dry etching. A resistively heated calibration device was made for temperature calibration of the thermal microprobe over the temperature range 25-110 °C. Over this range the thermal outputs of two microprobes are 4.5 and 5.6 μV/K and is linear. Thermal and topographical images are also obtained from a heated tungsten thin film fuse.

Embedded infrared fiber-optic sensor for thermometry in a high temperature/pressure environment

NASA Astrophysics Data System (ADS)

Yoo, Wook Jae; Jang, Kyoung Won; Moon, Jinsoo; Han, Ki-Tek; Jeon, Dayeong; Lee, Bongsoo; Park, Byung Gi

2012-11-01

In this study, we developed an embedded infrared fiber-optic temperature sensor for thermometry in high temperature/pressure and water-chemistry environments by using two identical silver-halide optical fibers. The performance of the fabricated temperature sensor was assessed in an autoclave filled with an aqueous coolant solution containing boric acid and lithium hydroxide. We carried out real-time monitoring of the infrared radiation emitted from the signal and reference probes for various temperatures over a temperature range from 95 to 225 °C. In order to decide the temperature of the synthetic coolant solution, we measured the difference between the infrared radiation emitted from the two temperature-sensing probes. Thermometry with the proposed sensor is immune to any changes in the physical conditions and the emissivity of the heat source. From the experimental results, the embedded infrared fiber-optic temperature sensor can withstand, and normally operate in a high temperature/pressure test loop system corresponding to the coolant system used for nuclear power plant simulation. We expect that the proposed sensor can be developed to accurately monitor temperatures in harsh environments.

Shielded piezoresistive cantilever probes for nanoscale topography and electrical imaging

NASA Astrophysics Data System (ADS)

Yang, Yongliang; Ma, Eric Yue; Cui, Yong-Tao; Haemmerli, Alexandre; Lai, Keji; Kundhikanjana, Worasom; Harjee, Nahid; Pruitt, Beth L.; Kelly, Michael; Shen, Zhi-Xun

2014-04-01

This paper presents the design and fabrication of piezoresistive cantilever probes for microwave impedance microscopy (MIM) to enable simultaneous topographic and electrical imaging. Plasma enhanced chemical vapor deposited Si3N4 cantilevers with a shielded center conductor line and nanoscale conductive tip apex are batch fabricated on silicon-on-insulator wafers. Doped silicon piezoresistors are integrated at the root of the cantilevers to sense their deformation. The piezoresistive sensitivity is 2 nm for a bandwidth of 10 kHz, enabling topographical imaging with reasonable speed. The aluminum center conductor has a low resistance (less than 5 Ω) and small capacitance (˜1.7 pF) to ground; these parameters are critical for high sensitivity MIM imaging. High quality piezoresistive topography and MIM images are simultaneously obtained with the fabricated probes at ambient and cryogenic temperatures. These new piezoresistive probes remarkably broaden the horizon of MIM for scientific applications by operating with an integrated feedback mechanism at low temperature and for photosensitive samples.

Practical polarization maintaining optical fibre temperature sensor for harsh environment application

NASA Astrophysics Data System (ADS)

Yang, Yuanhong; Xia, Haiyun; Jin, Wei

2007-10-01

A reflection spot temperature sensor was proposed based on the polarization mode interference in polarization maintaining optical fibre (PMF) and the phenomenon that the propagation constant difference of the two orthogonal polarization modes in stressing structures PMF is sensitive to temperature and the sensing equation was obtained. In this temperature sensor, a broadband source was used to suppress the drift due to polarization coupling in lead-in/lead-out PMF. A characteristic and performance investigation proved this sensor to be practical, flexible and precise. Experimental results fitted the theory model very well and the noise-limited minimum detectable temperature variation is less than 0.01 °C. The electric arc processing was investigated and the differential propagation constant modifying the PMF probe is performed. For the demand of field hot-spot monitoring of huge power transformers, a remote multi-channel temperature sensor prototype has been made and tested. Specially coated Panda PMF that can stand high temperatures up to 250 °C was fabricated and used as probe fibres. The sensor probes were sealed within thin quartz tubes that have high voltage insulation and can work in a hot oil and vapour environment. Test results show that the accuracy of the system is better than ±0.5 °C within 0 °C to 200 °C.

Pristine carbon nanotubes based resistive temperature sensor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Alam, Md Bayazeed, E-mail: bayazeed786@gmail.com; Jamia Millia Islamia; Saini, Sudhir Kumar, E-mail: sudhirsaini1310@gmail.com

A good sensor must be highly sensitive, faster in response, of low cost cum easily producible, and highly reliable. Incorporation of nano-dimensional particles/ wires makes conventional sensors more effective in terms of fulfilling the above requirements. For example, Carbon Nanotubes (CNTs) are promising sensing element because of its large aspect ratio, unique electronic and thermal properties. In addition to their use for widely reported chemical sensing, it has also been explored for temperature sensing. This paper presents the fabrication of CNTs based temperature sensor, prepared on silicon substrate using low cost spray coating method, which is reliable and reproducible methodmore » to prepare uniform CNTs thin films on any substrate. Besides this, simple and inexpensive method of preparation of dispersion of single walled CNTs (SWNTs) in 1,2 dichlorobenzene by using probe type ultrasonicator for debundling the CNTs for improving sensor response were used. The electrical contacts over the dispersed SWNTs were taken using silver paste electrodes. Fabricated sensors clearly show immediate change in resistance as a response to change in temperature of SWNTs. The measured sensitivity (change in resistance with temperature) of the sensor was found ∼ 0.29%/°C in the 25°C to 60°C temperature range.« less

Live-cell thermometry with nitrogen vacancy centers in nanodiamonds

NASA Astrophysics Data System (ADS)

Jayakumar, Harishankar; Fedder, Helmut; Chen, Andrew; Yang, Liudi; Li, Chenghai; Wrachtrup, Joerg; Wang, Sihong; Meriles, Carlos

The ability to measure temperature is typically affected by a tradeoff between sensitivity and spatial resolution. Good thermometers tend to be bulky systems and hence are ill-suited for thermal sensing with high spatial localization. Conversely, the signal resulting from nanoscale temperature probes is often impacted by noise to a level where the measurement precision becomes poor. Adding to the microscopist toolbox, the nitrogen vacancy (NV) center in diamond has recently emerged as a promising platform for high-sensitivity nanoscale thermometry. Of particular interest are applications in living cells because diamond nanocrystals are biocompatible and can be chemically functionalized to target specific organelles. Here we report progress on the ability to probe and compare temperature within and between living cells using nanodiamond-hosted NV thermometry. We focus our study on cancerous cells, where atypical metabolic pathways arguably lead to changes in the way a cell generates heat, and thus on its temperature profile.

Fibre Bragg grating encapted with no-core fibre sensors for SRI and temperature monitoring

NASA Astrophysics Data System (ADS)

Daud, S.; Amiri, I. S.; Noorden, A. F. A.; Ali, J.; Yupapin, P.

2018-06-01

In this work, a Fibre Bragg grating (FBG) encapted with no-core fibre (NCF) as surrounding refractive index (SRI) and temperature sensors are practically demonstrated. A FBG with 1550 nm wavelength was attached with 5 cm length of no-core fibre (NCF) is used as SRI and temperature sensing probe. The change of temperature and SRI induced the wavelength shift in FBG. The wavelength shift in FBG reacts directly proportional to the temperature with a sensitivity of while the sensitivity of NCF was measured as 13.13 pm °C-1.

Single-shot distributed Brillouin optical time domain analyzer.

PubMed

Fang, Jian; Xu, Pengbai; Dong, Yongkang; Shieh, William

2017-06-26

We demonstrate a novel single-shot distributed Brillouin optical time domain analyzer (SS-BOTDA). In our method, dual-polarization probe with orthogonal frequency-division multiplexing (OFDM) modulation is used to acquire the distributed Brillouin gain spectra, and coherent detection is used to enhance the signal-to-noise ratio (SNR) drastically. Distributed temperature sensing is demonstrated over a 1.08 km standard single-mode fiber (SSMF) with 20.48 m spatial resolution and 0.59 °C temperature accuracy. Neither frequency scanning, nor polarization scrambling, nor averaging is required in our scheme. All the data are obtained through only one-shot measurement, indicating that the sensing speed is only limited by the length of fiber.

Photonic all-silicon microsensor for electromagnetic power in the microwave and millimeter-wave range

NASA Astrophysics Data System (ADS)

Rendina, Ivo; Bellucci, Marco; Cocorullo, Giuseppe; Della Corte, Francesco G.; Iodice, Mario

2000-03-01

A new type of non-perturbing electromagnetic power sensor for microwaves and millimeter-waves, based on the thermo- optical effect in a silicon interferometric etalon cavity is presented. The incident field power is partially dissipated into the all-silicon metal-less etalon, constituting the sensing element of the detector, so causing its temperature increase. This, in turn, induces the intensity modulation of a probe laser beam reflected by the cavity after a multiple beam interference process. The sensing element is directly connected to an optical fiber for remote interrogation, so avoiding the use of perturbing coaxial cables. The performances of such a new class of non-perturbing and wideband probes, in terms of sensitivity and resolution are discussed in detail. The experimental results concerning the characterization of a preliminary prototype sensor are presented and compared with theoretical data. The dependence of the sensor response on the electromagnetic frequency and on the sensing element characteristics is finally discussed.

Flexible Cryogenic Temperature and Liquid-Level Probes

NASA Technical Reports Server (NTRS)

Haberbusch, Mark

2005-01-01

Lightweight, flexible probes have been developed for measuring temperatures at multiple locations in tanks that contain possibly pressurized cryogenic fluids. If the fluid in a given tank is subcritical (that is, if it consists of a liquid and its vapor), then in one of two modes of operation, the temperature measurements made by a probe of this type can be used to deduce the approximate level of the liquid. The temperature sensors are silicon diodes located at intervals along a probe. If the probe is to be used to measure a temperature gradient along a given axis in the tank, then the probe must be mounted along that axis. In the temperature-measurement mode, a constant small electric current is applied to each diode and the voltage across the diode a known function of the current and temperature is measured as an indication of its temperature. For the purpose of this measurement, small electric current signifies a current that is not large enough to cause a significant increase in the measured temperature. More specifically, the probe design calls for a current of 10 A, which, in the cryogenic temperature range of interest, generates heat at a rate of only about 0.01 mW per diode. In the liquid-level-sensing mode, one applies a larger current (30 mA) to each diode so as to heat each diode appreciably (with a power of about 36 mW in the temperature range of interest). Because the liquid cools the diode faster than does the vapor, the temperature of the diode is less when the diode is immersed in the liquid than when it is above the surface of the liquid. Thus, the temperature (voltage) reading from each diode can be used to determine whether the liquid level is above or below the diode, and one can deduce that the liquid level lies between two adjacent diodes, the lower one of which reads a significantly lower temperature. The aforementioned techniques for measuring temperature and deducing liquid level are not new. What is new here are the designs of the probes and of associated external electronic circuitry. In each probe, the diodes and the lead wires are embedded in a strong, lightweight, flexible polyimide strip. Each probe is constructed as an integral unit that includes a multipin input/output plug or socket for solderless connection of the lead wires to the external circuitry. The polyimide strip includes mounting tabs with holes that can accommodate rivets, screws, or other fasteners. Alternatively, a probe can be mounted by use of an epoxy. A probe can be manufactured to almost any length or width, and the diodes can be embedded at almost any desired location along and across the polyimide strip. In designing a probe for a specific application, one seeks a compromise between (1) minimizing the number of diodes in order to minimize the complexity of input/output connections and external electronic circuitry while (2) using enough diodes to obtain the required precision. Optionally, to minimize spurious heating of the cryogenic fluid, the external circuitry can be designed to apply power to the probe only during brief measurement intervals. Assuming that the external circuitry is maintained at a steady temperature, a power-on interval of only a few seconds is sufficient to obtain accurate data on temperatures and/or the height of the liquid/vapor interface.

Fluorescent probes and nanoparticles for intracellular sensing of pH values

NASA Astrophysics Data System (ADS)

Shi, Wen; Li, Xiaohua; Ma, Huimin

2014-12-01

Intracellular pH regulates a number of cell metabolism processes and its sensing is thus of great importance for cell studies. Among various methods, fluorescent probes have been widely used for sensing intracellular pH values because of their high sensitivity and spatiotemporal resolution capability. In this article, the development of fluorescent probes with good practicability in sensing intracellular pH values and pH variation during 2009 - 2014 is reviewed. These fluorescence probes are divided into two kinds: small molecules and nanoparticles. Photophysical properties, advantages/disadvantages and applications of the two kinds of probes are discussed in detail.

Optical display for radar sensing

NASA Astrophysics Data System (ADS)

Szu, Harold; Hsu, Charles; Willey, Jefferson; Landa, Joseph; Hsieh, Minder; Larsen, Louis V.; Krzywicki, Alan T.; Tran, Binh Q.; Hoekstra, Philip; Dillard, John T.; Krapels, Keith A.; Wardlaw, Michael; Chu, Kai-Dee

2015-05-01

Boltzmann headstone S = kB Log W turns out to be the Rosette stone for Greek physics translation optical display of the microwave sensing hieroglyphics. The LHS is the molecular entropy S measuring the degree of uniformity scattering off the sensing cross sections. The RHS is the inverse relationship (equation) predicting the Planck radiation spectral distribution parameterized by the Kelvin temperature T. Use is made of the conservation energy law of the heat capacity of Reservoir (RV) change T Δ S = -ΔE equals to the internal energy change of black box (bb) subsystem. Moreover, an irreversible thermodynamics Δ S > 0 for collision mixing toward totally larger uniformity of heat death, asserted by Boltzmann, that derived the so-called Maxwell-Boltzmann canonical probability. Given the zero boundary condition black box, Planck solved a discrete standing wave eigenstates (equation). Together with the canonical partition function (equation) an average ensemble average of all possible internal energy yielded the celebrated Planck radiation spectral (equation) where the density of states (equation). In summary, given the multispectral sensing data (equation), we applied Lagrange Constraint Neural Network (LCNN) to solve the Blind Sources Separation (BSS) for a set of equivalent bb target temperatures. From the measurements of specific value, slopes and shapes we can fit a set of Kelvin temperatures T's for each bb targets. As a result, we could apply the analytical continuation for each entropy sources along the temperature-unique Planck spectral curves always toward the RGB color temperature display for any sensing probing frequency.

Tungsten-Doped TiO2 Nanolayers with Improved CO2 Gas Sensing Properties for Environmental Applications

NASA Astrophysics Data System (ADS)

Saberi, Maliheh; Ashkarran, Ali Akbar

Tungsten-doped TiO2 gas sensors were successfully synthesized using sol-gel process and spin coating technique. The fabricated sensor was characterized by field emission scanning electron microscopy (FE-SEM), ultraviolet visible (UV-Vis) spectroscopy, transmission electron microscopy (TEM), X-Ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. Gas sensing properties of pristine and tungsten-doped TiO2 nanolayers (NLs) were probed by detection of CO2 gas. A series of experiments were conducted in order to find the optimum operating temperature of the prepared sensors and also the optimum value of tungsten concentration in TiO2 matrix. It was found that introducing tungsten into the TiO2 matrix enhanced the gas sensing performance. The maximum response was found to be (1.37) for 0.001g tungsten-doped TiO2 NLs at 200∘C as an optimum operating temperature.

Development of ultrasound-assisted fluorescence imaging of indocyanine green.

PubMed

Morikawa, Hiroyasu; Toyota, Shin; Wada, Kenji; Uchida-Kobayashi, Sawako; Kawada, Norifumi; Horinaka, Hiromichi

2017-01-01

Indocyanine green (ICG) accumulation in hepatocellular carcinoma means tumors can be located by fluorescence. However, because of light scattering, it is difficult to detect ICG fluorescence from outside the body. We propose a new fluorescence imaging method that detects changes in the intensity of ICG fluorescence by ultrasound-induced temperature changes. ICG fluorescence intensity decreases as the temperature rises. Therefore, it should theoretically be possible to detect tissue distribution of ICG using ultrasound to heat tissue, moving the point of ultrasound transmission, and monitoring changes in fluorescence intensity. A new probe was adapted for clinical application. It consisted of excitation light from a laser, fluorescence sensing through a light pipe, and heating by ultrasound. We applied the probe to bovine liver to image the accumulation of ICG. ICG emits fluorescence (820 nm) upon light irradiation (783 nm). With a rise in temperature, the fluorescence intensity of ICG decreased by 0.85 %/°C. The distribution of fluorescent ICG was detected using an ultrasonic warming method in a new integrated probe. Modulating fluorescence by changing the temperature using ultrasound can determine where ICG accumulates at a depth, highlighting its potential as a means to locate hepatocellular carcinoma.

Sulfophenyl-Functionalized Reduced Graphene Oxide Networks on Electrospun 3D Scaffold for Ultrasensitive NO₂ Gas Sensor.

PubMed

Zou, Bin; Guo, Yunlong; Shen, Nannan; Xiao, Anshan; Li, Mingjun; Zhu, Liang; Wan, Pengbo; Sun, Xiaoming

2017-12-19

Ultrasensitive room temperature real-time NO₂ sensors are highly desirable due to potential threats on environmental security and personal respiratory. Traditional NO₂ gas sensors with highly operated temperatures (200-600 °C) and limited reversibility are mainly constructed from semiconducting oxide-deposited ceramic tubes or inter-finger probes. Herein, we report the functionalized graphene network film sensors assembled on an electrospun three-dimensional (3D) nanonetwork skeleton for ultrasensitive NO₂ sensing. The functional 3D scaffold was prepared by electrospinning interconnected polyacrylonitrile (PAN) nanofibers onto a nylon window screen to provide a 3D nanonetwork skeleton. Then, the sulfophenyl-functionalized reduced graphene oxide (SFRGO) was assembled on the electrospun 3D nanonetwork skeleton to form SFRGO network films. The assembled functionalized graphene network film sensors exhibit excellent NO₂ sensing performance (10 ppb to 20 ppm) at room temperature, reliable reversibility, good selectivity, and better sensing cycle stability. These improvements can be ascribed to the functionalization of graphene with electron-withdrawing sulfophenyl groups, the high surface-to-volume ratio, and the effective sensing channels from SFRGO wrapping onto the interconnected 3D scaffold. The SFRGO network-sensing film has the advantages of simple preparation, low cost, good processability, and ultrasensitive NO₂ sensing, all advantages that can be utilized for potential integration into smart windows and wearable electronic devices for real-time household gas sensors.

Digital hand-held temperature monitor

NASA Astrophysics Data System (ADS)

Allin, L. V.; Ferrari, I.

1980-09-01

A hand-held non-invasive monitoring instrument has been designed, constructed and tested to allow core temperature measurements to be obtained from human subjects who have swallowed a temperature-sensing radio transmitter (radio pill). This instrument uses a simple AM radio for a receiver, digital circuitry to decode the received signal and a four-digit LED module to display the temperature. The unit, which is battery-powered, can be held in one hand while an antenna probe is swept over the abdomen of the subject until a continuously audible signal is generated by a piezoelectric sound source, indicating reception. The digital display then presents the body core temperature in tenths of a degree Celsius.

Bi-Directional Brillouin Optical Time Domain Analyzer System for Long Range Distributed Sensing.

PubMed

Guo, Nan; Wang, Liang; Wang, Jie; Jin, Chao; Tam, Hwa-Yaw; Zhang, A Ping; Lu, Chao

2016-12-16

We propose and experimentally demonstrate a novel scheme of bi-directional Brillouin time domain analyzer (BD-BOTDA) to extend the sensing range. By deploying two pump-probe pairs at two different wavelengths, the Brillouin frequency shift (BFS) distribution over each half of the whole fiber can be obtained with the simultaneous detection of Brillouin signals in both channels. Compared to the conventional unidirectional BOTDA system of the same sensing range, the proposed BD-BOTDA scheme enables distributed sensing with a performance level comparable to the conventional one with half of the sensing range and a spatial resolution of 2 m, while maintaining the Brillouin signal-to-noise ratio (SNR) and the BFS uncertainty. Based on this technique, we have achieved distributed temperature sensing with a measurement range of 81.9 km fiber at a spatial resolution of 2 m and BFS uncertainty of ~0.44 MHz without introducing any complicated components or schemes.

Exploring luminescence-based temperature sensing using protein-passivated gold nanoclusters

NASA Astrophysics Data System (ADS)

Chen, Xi; Essner, Jeremy B.; Baker, Gary A.

2014-07-01

We explore the analytical performance and limitations of optically monitoring aqueous-phase temperature using protein-protected gold nanoclusters (AuNCs). Although not reported elsewhere, we find that these bio-passivated AuNCs show pronounced hysteresis upon thermal cycling. This unwanted behaviour can be eliminated by several strategies, including sol-gel coating and thermal denaturation of the biomolecular template, introducing protein-templated AuNC probes as viable nanothermometers.We explore the analytical performance and limitations of optically monitoring aqueous-phase temperature using protein-protected gold nanoclusters (AuNCs). Although not reported elsewhere, we find that these bio-passivated AuNCs show pronounced hysteresis upon thermal cycling. This unwanted behaviour can be eliminated by several strategies, including sol-gel coating and thermal denaturation of the biomolecular template, introducing protein-templated AuNC probes as viable nanothermometers. Electronic supplementary information (ESI) available: Supplemental figures and experimental details. See DOI: 10.1039/c4nr02069c

Optical Probe of the Superconducting Normal Mixed State in a Magnetic Penetration Thermometer

NASA Technical Reports Server (NTRS)

Stevenson, T. R.; Balvin, M. A.; Bandler, S. R.; Denis, K. L.; Lee, S. -J.; Nagler, P. C.; Smith, S. J.

2016-01-01

Using ultraviolet photon pulses, we have probed the internal behavior of a molybdenum-gold Magnetic Penetration Thermometer (MPT) that we designed for x-ray microcalorimetry. In this low-temperature detector, the diamagnetic response of a superconducting MoAu bilayer is used to sense temperature changes in response to absorbed photons. We have previously described an approximate model that explains the high responsivity of the detector to temperature changes as a consequence of a Meissner transition of the molybdenum-gold film in the magnetic field applied by the superconducting circuit used to bias the detector. We compare measurements of MPT heat capacity and thermal conductance, derived from UV photon pulse data, to our model predictions for the thermodynamic properties of the sensor and for the electron cooling obtained by quasiparticle recombination. Our data on electron cooling power is also relevant to the operation of other superconducting detectors, such as Microwave Kinetic Inductance Detectors.

Energy-Based Tetrahedron Sensor for High-Temperature, High-Pressure Environments

NASA Technical Reports Server (NTRS)

Gee, Kent L.; Sommerfeldt, Scott D.; Blotter, Jonathan D.

2012-01-01

An acoustic energy-based probe has been developed that incorporates multiple acoustic sensing elements in order to obtain the acoustic pressure and three-dimensional acoustic particle velocity. With these quantities, the user can obtain various energy-based quantities, including acoustic energy density, acoustic intensity, and acoustic impedance. In this specific development, the probe has been designed to operate in an environment characterized by high temperatures and high pressures as is found in the close vicinity of rocket plumes. Given these capabilities, the probe is designed to be used to investigate the acoustic conditions within the plume of a rocket engine or jet engine to facilitate greater understanding of the noise generation mechanisms in those plumes. The probe features sensors mounted inside a solid sphere. The associated electronics for the probe are contained within the sphere and the associated handle for the probe. More importantly, the design of the probe has desirable properties that reduce the bias errors associated with determining the acoustic pressure and velocity using finite sum and difference techniques. The diameter of the probe dictates the lower and upper operating frequencies for the probe, where accurate measurements can be acquired. The current probe design implements a sphere diameter of 1 in. (2.5 cm), which limits the upper operating frequency to about 4.5 kHz. The sensors are operational up to much higher frequencies, and could be used to acquire pressure data at higher frequencies, but the energy-based measurements are limited to that upper frequency. Larger or smaller spherical probes could be designed to go to lower or higher frequency range

Integrated control and health monitoring capacitive displacement sensor development task. Orbit transfer rocket engine technology program

NASA Technical Reports Server (NTRS)

Collamore, Frank N.

1989-01-01

The development of a miniature multifunction turbomachinery shaft displacement sensor using state-of-the-art non-contract capacitive sensing technology is described. Axial displacement, radial displacement, and speed are sensed using a single probe within the envelope normally required for a single function. A survey of displacement sensing technology is summarized including inductive, capacitive, optical and ultrasonic techniques. The design and operation of an experimental triple function sensor is described. Test results are included showing calibration tests and simultaneous dynamic testing of multiple functions. Recommendations for design changes are made to improve low temperature performance, reliability, and for design of a flight type signal conditioning unit.

A gold nanocluster-based fluorescent probe for simultaneous pH and temperature sensing and its application to cellular imaging and logic gates

NASA Astrophysics Data System (ADS)

Wu, Yun-Tse; Shanmugam, Chandirasekar; Tseng, Wei-Bin; Hiseh, Ming-Mu; Tseng, Wei-Lung

2016-05-01

Metal nanocluster-based nanomaterials for the simultaneous determination of temperature and pH variations in micro-environments are still a challenge. In this study, we develop a dual-emission fluorescent probe consisting of bovine serum albumin-stabilized gold nanoclusters (BSA-AuNCs) and fluorescein-5-isothiocyanate (FITC) as temperature- and pH-responsive fluorescence signals. Under single wavelength excitation the FITC/BSA-AuNCs exhibited well-separated dual emission bands at 525 and 670 nm. When FITC was used as a reference fluorophore, FITC/BSA-AuNCs showed a good linear response over the temperature range 1-71 °C and offered temperature-independent spectral shifts, temperature accuracy, activation energy, and reusability. The possible mechanism for high temperature-induced fluorescence quenching of FITC/BSA-AuNCs could be attributed to a weakening of the Au-S bond, thereby lowering the charge transfer from BSA to AuNCs. Additionally, the pH- and temperature-responsive properties of FITC/BSA-AuNCs allow simultaneous temperature sensing from 21 to 41 °C (at intervals of 5 °C) and pH from 6.0 to 8.0 (at intervals of 0.5 pH unit), facilitating the construction of two-input AND logic gates. Three-input AND logic gates were also designed using temperature, pH, and trypsin as inputs. The practicality of using FITC/BSA-AuNCs to determine the temperature and pH changes in HeLa cells is also validated.Metal nanocluster-based nanomaterials for the simultaneous determination of temperature and pH variations in micro-environments are still a challenge. In this study, we develop a dual-emission fluorescent probe consisting of bovine serum albumin-stabilized gold nanoclusters (BSA-AuNCs) and fluorescein-5-isothiocyanate (FITC) as temperature- and pH-responsive fluorescence signals. Under single wavelength excitation the FITC/BSA-AuNCs exhibited well-separated dual emission bands at 525 and 670 nm. When FITC was used as a reference fluorophore, FITC/BSA-AuNCs showed a good linear response over the temperature range 1-71 °C and offered temperature-independent spectral shifts, temperature accuracy, activation energy, and reusability. The possible mechanism for high temperature-induced fluorescence quenching of FITC/BSA-AuNCs could be attributed to a weakening of the Au-S bond, thereby lowering the charge transfer from BSA to AuNCs. Additionally, the pH- and temperature-responsive properties of FITC/BSA-AuNCs allow simultaneous temperature sensing from 21 to 41 °C (at intervals of 5 °C) and pH from 6.0 to 8.0 (at intervals of 0.5 pH unit), facilitating the construction of two-input AND logic gates. Three-input AND logic gates were also designed using temperature, pH, and trypsin as inputs. The practicality of using FITC/BSA-AuNCs to determine the temperature and pH changes in HeLa cells is also validated. Electronic supplementary information (ESI) available. See DOI: 10.1039/c6nr02341j

A milliKelvin scanning Hall probe microscope for high resolution magnetic imaging

NASA Astrophysics Data System (ADS)

Khotkevych, V. V.; Bending, S. J.

2009-02-01

The design and performance of a novel scanning Hall probe microscope for milliKelvin magnetic imaging with submicron lateral resolution is presented. The microscope head is housed in the vacuum chamber of a commercial 3He-refrigerator and operates between room temperature and 300 mK in magnetic fields up to 10 T. Mapping of the local magnetic induction at the sample surface is performed by a micro-fabricated 2DEG Hall probe equipped with an integrated STM tip. The latter provides a reliable mechanism of surface tracking by sensing and controlling the tunnel currents. We discuss the results of tests of the system and illustrate its potential with images of suitable reference samples captured in different modes of operation.

Dual-probe near-field fiber head with gap servo control for data storage applications.

PubMed

Fang, Jen-Yu; Tien, Chung-Hao; Shieh, Han-Ping D

2007-10-29

We present a novel fiber-based near-field optical head consisting of a straw-shaped writing probe and a flat gap sensing probe. The straw-shaped probe with a C-aperture on the end face exhibits enhanced transmission by a factor of 3 orders of magnitude over a conventional fiber probe due to a hybrid effect that excites both propagation modes and surface plasmon waves. In the gap sensing probe, the spacing between the probe and the disk surface functions as an external cavity. The high sensitivity of the output power to the change in the gap width is used as a feedback control signal. We characterize and design the straw-shaped writing probe and the flat gap sensing probe. The dual-probe system is installed on a conventional biaxial actuator to demonstrate the capability of flying over a disk surface with nanometer position precision.

Understanding of Jupiter's Atmosphere after the Galileo Probe Entry

NASA Technical Reports Server (NTRS)

Fonda, Mark (Technical Monitor); Young, Richard E.

2003-01-01

Instruments on the Galileo probe measured composition, cloud properties, thermal structure, winds, radiative energy balance, and electrical properties of the Jovian atmosphere. As expected the probe results confirm some expectations about Jupiter's atmosphere, refute others, and raise new questions which still remain unanswered. This talk will concentrate on those aspects of the probe observations which either raised new questions or remain unresolved. The Galileo probe observations of composition and clouds provided some of the biggest surprises of the mission. Helium abundance measured by the probe differed significantly from the remote sensing derivations from Voyager. Discrepancy between the Voyager helium abundance determinations for Jupiter and the Galileo probe value have now led to a considerably increased helium determination for Saturn. Global abundance of N in the form of ammonia was observed to be super-solar by approximately the same factor as carbon, in contrast to expectations that C/N would be significantly larger than solar. This has implications for the formation and evolution of Jupiter. The cloud structure was not what was generally anticipated, even though most previous remote sensing results below the uppermost cloud referred to 5 micron hot spots, local regions with reduced cloud opacity. The Galileo probe descended in one of these hot spots. Only a tenuous, presumed ammomium hydrosulfide, cloud was detected, and no significant water cloud or super-solar water abundance was measured. The mixing ratios as a function of depth for the condensibles ammonia, hydrogen sulfide, and water, exhibited no apparent correlation with either condensation levels or with each other, an observation that is still a puzzle, although there are now dynamical models of hot spots which show promise in being able to explain such behavior. Probe tracked zonal winds show that wind magnitude increases with depth to pressures of about 4 bars, with the winds extending to at least as deep as the probe made measurements, 22 bars. Models of hot spot dynamics raise the possibility that the variation with depth of the probe measured zonal winds between 0.4 and 4 bars reflect the dynamics of the hot spot rather than the global wind pattern. Galileo upper atmosphere measurements established that there is a sharp temperature rise with altitude between about 350 and 800 km above the 1 bar pressure level, with the upper atmosphere reaching temperatures near 900 K. The energy sources for this upper atmosphere heating are not clearly established, but various mechanisms have been proposed. These and other aspects of the Galileo probe data will be discussed.

Understanding of Jupiter's Atmosphere After the Galileo Probe Entry

NASA Technical Reports Server (NTRS)

Young, Richard E.; DeVincenzi, Donald (Technical Monitor)

2001-01-01

Instruments on the Galileo probe measured composition, cloud properties, thermal structure. winds, radiative energy balance, and electrical properties of the Jovian atmosphere. As expected the probe results confirm some expectations about Jupiter's atmosphere, refute others, and raise new questions which still remain unanswered. This talk will concentrate on those aspects of the probe observations which either raised new questions or remain unresolved. The Galileo probe observations of composition and clouds provided some of the biggest surprises of the mission. Helium abundance measured by the probe differed significantly from the remote sensing derivations from Voyager. discrepancy between the Voyager helium abundance determinations for Jupiter and the Galileo probe value have now led to a considerably increased helium determination for Saturn. Global abundance of N in the form of ammonia was observed to be supersolar by approximately the same factor as carbon, in contrast to expectations that C/N would be significantly larger than solar. This has implications for the formation and evolution of Jupiter. The cloud structure was not what was generally anticipated, even though most previous remote sensing results below the uppermost cloud referred to 5 micron hot spots, local regions with reduced cloud opacity. The Galileo probe descended in one of these hot spots. Only a tenuous, presumed ammonium hydrosulfide, cloud was detected, and no significant water cloud or super-solar water abundance was measured. The mixing ratios as a function of depth for the condensibles ammonia, hydrogen sulfide, and water, exhibited no apparent correlation with either condensation levels or with each other, an observation that is still a puzzle, although there are now dynamical models of hot spots which show promise in being able to explain such behavior. Probe tracked zonal winds show that wind magnitude increases with depth to pressures of about 4 bars, with the winds extending to at least as deep as the probe made measurements, 22 bars. Models of hot spot dynamics raise the possibility that the variation with depth of the probe measured zonal winds between 0.4 and 4 bars reflect the dynamics of the hot spot rather than the global wind pattern. Galileo upper atmosphere measurements established that there is a sharp temperature rise with altitude between about 350 and 800 km above the 1 bar pressure level, with the upper atmosphere reaching, temperatures near 900 K. The energy sources for this upper atmosphere heating are not clearly established, but various mechanisms have been proposed. These and other aspects of the Galileo probe data will be discussed.

Gate-Sensing the Potential Landscape of a GaAs Two-Dimensional Electron Gas

NASA Astrophysics Data System (ADS)

Croot, Xanthe; Mahoney, Alice; Pauka, Sebastian; Colless, James; Reilly, David; Watson, John; Fallahi, Saeed; Gardner, Geoff; Manfra, Michael; Lu, Hong; Gossard, Arthur

In situ dispersive gate sensors hold potential as a means of enabling the scalable readout of quantum dot arrays. Sensitive to quantum capacitance, dispersive sensors have been used to detect inter- and intra-dot transitions in GaAs double quantum dots, and can distinguish the spin states of singlet triplet qubits. In addition, the gate-sensing technique is likely of value in probing the physics of Majorana zero modes in nanowire devices. Beyond the readout signatures associated with charge and spin configurations of qubits, gate-sensing is sensitive to trapped charge in the potential landscape. Here, we report gate-sensing signals arising from tunnelling of electrons between puddles of trapped charge in a GaAs 2DEG. We examine these signals in a family of different devices with varying mobilities, and as a function of temperature and bias. Implications for qubit readout using the gate-sensing technique are discussed.

Hydrogel-Based Fluorescent Dual pH and Oxygen Sensors Loaded in 96-Well Plates for High-Throughput Cell Metabolism Studies.

PubMed

Wu, Shanshan; Wu, Siying; Yi, Zheyuan; Zeng, Fei; Wu, Weizhen; Qiao, Yuan; Zhao, Xingzhong; Cheng, Xing; Tian, Yanqing

2018-02-13

In this study, we developed fluorescent dual pH and oxygen sensors loaded in multi-well plates for in-situ and high-throughput monitoring of oxygen respiration and extracellular acidification during microbial cell growth for understanding metabolism. Biocompatible PHEMA-co-PAM materials were used as the hydrogel matrix. A polymerizable oxygen probe (OS2) derived from PtTFPP and a polymerizable pH probe (S2) derived from fluorescein were chemically conjugated into the matrix to solve the problem of the probe leaching from the matrix. Gels were allowed to cure directly on the bottom of 96-well plates at room-temperature via redox polymerization. The influence of matrix's composition on the sensing behaviors was investigated to optimize hydrogels with enough robustness for repeatable use with good sensitivity. Responses of the dual sensing hydrogels to dissolved oxygen (DO) and pH were studied. These dual oxygen-pH sensing plates were successfully used for microbial cell-based screening assays, which are based on the measurement of fluorescence intensity changes induced by cellular oxygen consumption and pH changes during microbial growth. This method may provide a real-time monitoring of cellular respiration, acidification, and a rapid kinetic assessment of multiple samples for cell viability as well as high-throughput drug screening. All of these assays can be carried out by a conventional plate reader.

Multiparametric methane sensor for environmental monitoring

NASA Astrophysics Data System (ADS)

Borecki, M.; Duk, M.; Kociubiński, A.; Korwin-Pawlowski, M. L.

2016-12-01

Today, methane sensors find applications mostly in safety alarm installations, gas parameters detection and air pollution classification. Such sensors and sensors elements exists for industry and home use. Under development area of methane sensors application is dedicated to ground gases monitoring. Proper monitoring of soil gases requires reliable and maintenance-free semi-constant and longtime examination at relatively low cost of equipment. The sensors for soil monitoring have to work on soil probe. Therefore, sensor is exposed to environment conditions, as a wide range of temperatures and a full scale of humidity changes, as well as rain, snow and wind, that are not specified for classical methane sensors. Development of such sensor is presented in this paper. The presented sensor construction consists of five commercial non dispersive infra-red (NDIR) methane sensing units, a set of temperature and humidity sensing units, a gas chamber equipped with a micro-fan, automated gas valves and also a microcontroller that controls the measuring procedure. The electronics part of sensor was installed into customized 3D printed housing equipped with self-developed gas valves. The main development of proposed sensor is on the side of experimental evaluation of construction reliability and results of data processing included safety procedures and function for hardware error correction. Redundant methane sensor units are used providing measurement error correction as well as improved measurement accuracy. The humidity and temperature sensors are used for internal compensation of methane measurements as well as for cutting-off the sensor from the environment when the conditions exceed allowable parameters. Results obtained during environment sensing prove that the gas concentration readings are not sensitive to gas chamber vertical or horizontal position. It is important as vertical sensor installation on soil probe is simpler that horizontal one. Data acquired during six month of environment monitoring prove that error correction of methane sensing units was essential for maintenance free sensor operation, despite used safety procedures.

“Turn-on” fluorescence probe integrated polymer nanoparticles for sensing biological thiol molecules

NASA Astrophysics Data System (ADS)

Ang, Chung Yen; Tan, Si Yu; Lu, Yunpeng; Bai, Linyi; Li, Menghuan; Li, Peizhou; Zhang, Quan; Selvan, Subramanian Tamil; Zhao, Yanli

2014-11-01

A ``turn-on'' thiol-responsive fluorescence probe was synthesized and integrated into polymeric nanoparticles for sensing intracellular thiols. There is a photo-induced electron transfer process in the off state of the probe, and this process is terminated upon the reaction with thiol compounds. Configuration interaction singles (CIS) calculation was performed to confirm the mechanism of this process. A series of sensing studies were carried out, showing that the probe-integrated nanoparticles were highly selective towards biological thiol compounds over non-thiolated amino acids. Kinetic studies were also performed to investigate the relative reaction rate between the probe and the thiolated amino acids. Subsequently, the Gibbs free energy of the reactions was explored by means of the electrochemical method. Finally, the detection system was employed for sensing intracellular thiols in cancer cells, and the sensing selectivity could be further enhanced with the use of a cancer cell-targeting ligand in the nanoparticles. This development paves a path for the sensing and detection of biological thiols, serving as a potential diagnostic tool in the future.

System and method for moving a probe to follow movements of tissue

NASA Technical Reports Server (NTRS)

Feldstein, C.; Andrews, T. W.; Crawford, D. W.; Cole, M. A. (Inventor)

1981-01-01

An apparatus is described for moving a probe that engages moving living tissue such as a heart or an artery that is penetrated by the probe, which moves the probe in synchronism with the tissue to maintain the probe at a constant location with respect to the tissue. The apparatus includes a servo positioner which moves a servo member to maintain a constant distance from a sensed object while applying very little force to the sensed object, and a follower having a stirrup at one end resting on a surface of the living tissue and another end carrying a sensed object adjacent to the servo member. A probe holder has one end mounted on the servo member and another end which holds the probe.

Nanoscale Probing of Thermal, Stress, and Optical Fields under Near-Field Laser Heating

PubMed Central

Tang, Xiaoduan; Xu, Shen; Wang, Xinwei

2013-01-01

Micro/nanoparticle induced near-field laser ultra-focusing and heating has been widely used in laser-assisted nanopatterning and nanolithography to pattern nanoscale features on a large-area substrate. Knowledge of the temperature and stress in the nanoscale near-field heating region is critical for process control and optimization. At present, probing of the nanoscale temperature, stress, and optical fields remains a great challenge since the heating area is very small (∼100 nm or less) and not immediately accessible for sensing. In this work, we report the first experimental study on nanoscale mapping of particle-induced thermal, stress, and optical fields by using a single laser for both near-field excitation and Raman probing. The mapping results based on Raman intensity variation, wavenumber shift, and linewidth broadening all give consistent conjugated thermal, stress, and near-field focusing effects at a 20 nm resolution (<λ/26, λ = 32 nm). Nanoscale mapping of near-field effects of particles from 1210 down to 160 nm demonstrates the strong capacity of such a technique. By developing a new strategy for physical analysis, we have de-conjugated the effects of temperature, stress, and near-field focusing from the Raman mapping. The temperature rise and stress in the nanoscale heating region is evaluated at different energy levels. High-fidelity electromagnetic and temperature field simulation is conducted to accurately interpret the experimental results. PMID:23555566

Can we settle with single-band radiometric temperature monitoring during hyperthermia treatment of chestwall recurrence of breast cancer using a dual-mode transceiving applicator?

PubMed

Jacobsen, Svein; Stauffer, Paul R

2007-02-21

The total thermal dose that can be delivered during hyperthermia treatments is frequently limited by temperature heterogeneities in the heated tissue volume. Reliable temperature information on the heated area is thus vital for the optimization of clinical dosimetry. Microwave radiometry has been proposed as an accurate, quick and painless temperature sensing technique for biological tissue. Advantages include the ability to sense volume-averaged temperatures from subsurface tissue non-invasively, rather than with a limited set of point measurements typical of implanted temperature probes. We present a procedure to estimate the maximum tissue temperature from a single radiometric brightness temperature which is based on a numerical simulation of 3D tissue temperature distributions induced by microwave heating at 915 MHz. The temperature retrieval scheme is evaluated against errors arising from unknown variations in thermal, electromagnetic and design model parameters. Whereas realistic deviations from base values of dielectric and thermal parameters have only marginal impact on performance, pronounced deviations in estimated maximum tissue temperature are observed for unanticipated variations of the temperature or thickness of the bolus compartment. The need to pay particular attention to these latter applicator construction parameters in future clinical implementation of the thermometric method is emphasized.

Can we settle with single-band radiometric temperature monitoring during hyperthermia treatment of chestwall recurrence of breast cancer using a dual-mode transceiving applicator?

NASA Astrophysics Data System (ADS)

Jacobsen, Svein; Stauffer, Paul R.

2007-02-01

The total thermal dose that can be delivered during hyperthermia treatments is frequently limited by temperature heterogeneities in the heated tissue volume. Reliable temperature information on the heated area is thus vital for the optimization of clinical dosimetry. Microwave radiometry has been proposed as an accurate, quick and painless temperature sensing technique for biological tissue. Advantages include the ability to sense volume-averaged temperatures from subsurface tissue non-invasively, rather than with a limited set of point measurements typical of implanted temperature probes. We present a procedure to estimate the maximum tissue temperature from a single radiometric brightness temperature which is based on a numerical simulation of 3D tissue temperature distributions induced by microwave heating at 915 MHz. The temperature retrieval scheme is evaluated against errors arising from unknown variations in thermal, electromagnetic and design model parameters. Whereas realistic deviations from base values of dielectric and thermal parameters have only marginal impact on performance, pronounced deviations in estimated maximum tissue temperature are observed for unanticipated variations of the temperature or thickness of the bolus compartment. The need to pay particular attention to these latter applicator construction parameters in future clinical implementation of the thermometric method is emphasized.

Ferromagnetic resonance probe liftoff suppression apparatus

DOEpatents

Davis, Thomas J.; Tomeraasen, Paul L.

1985-01-01

A liftoff suppression apparatus utilizing a liftoff sensing coil to sense the amount a ferromagnetic resonance probe lifts off the test surface during flaw detection and utilizing the liftoff signal to modulate the probe's field modulating coil to suppress the liftoff effects.

Fabrication and surface-modification of implantable microprobes for neuroscience studies

NASA Astrophysics Data System (ADS)

Cao, H.; Nguyen, C. M.; Chiao, J. C.

2012-06-01

In this work implantable micro-probes for central nervous system (CNS) studies were developed on silicon and polyimide substrates. The probes which contained micro-electrode arrays with different surface modifications were designed for implantation in the CNS. The electrode surfaces were modified with nano-scale structures that could greatly increase the active surface area in order to enhance the electrochemical current outputs while maintaining micro-scale dimensions of the electrodes and probes. The electrodes were made of gold or platinum, and designed with different sizes. The silicon probes were modified by silicon nanowires fabricated with the vapor-liquid-solid mechanism at high temperatures. With polyimide substrates, the nanostructure modification was carried out by applying concentrated gold or silver colloid solutions onto the micro-electrodes at room temperature. The surfaces of electrodes before and after modification were observed by scanning electron microscopy. The silicon nanowire-modified surface was characterized by cyclic voltammetry. Experiments were carried out to investigate the improvement in sensing performance. The modified electrodes were tested with H2O2, electrochemical L-glutamate and dopamine. Comparisons between electrodes with and without nanostructure modification were conducted showing that the modifications have enhanced the signal outputs of the electrochemical neurotransmitter sensors.

Self-sensing of temperature rises on light emitting diode based optrodes

NASA Astrophysics Data System (ADS)

Dehkhoda, Fahimeh; Soltan, Ahmed; Ponon, Nikhil; Jackson, Andrew; O'Neill, Anthony; Degenaar, Patrick

2018-04-01

Objective. This work presents a method to determine the surface temperature of microphotonic medical implants like LEDs. Our inventive step is to use the photonic emitter (LED) employed in an implantable device as its own sensor and develop readout circuitry to accurately determine the surface temperature of the device. Approach. There are two primary classes of applications where microphotonics could be used in implantable devices; opto-electrophysiology and fluorescence sensing. In such scenarios, intense light needs to be delivered to the target. As blue wavelengths are scattered strongly in tissue, such delivery needs to be either via optic fibres, two-photon approaches or through local emitters. In the latter case, as light emitters generate heat, there is a potential for probe surfaces to exceed the 2 °C regulatory. However, currently, there are no convenient mechanisms to monitor this in situ. Main results. We present the electronic control circuit and calibration method to monitor the surface temperature change of implantable optrode. The efficacy is demonstrated in air, saline, and brain. Significance. This paper, therefore, presents a method to utilize the light emitting diode as its own temperature sensor.

Challenges and Opportunities for Small-Molecule Fluorescent Probes in Redox Biology Applications.

PubMed

Jiang, Xiqian; Wang, Lingfei; Carroll, Shaina L; Chen, Jianwei; Wang, Meng C; Wang, Jin

2018-02-16

The concentrations of reactive oxygen/nitrogen species (ROS/RNS) are critical to various biochemical processes. Small-molecule fluorescent probes have been widely used to detect and/or quantify ROS/RNS in many redox biology studies and serve as an important complementary to protein-based sensors with unique applications. Recent Advances: New sensing reactions have emerged in probe development, allowing more selective and quantitative detection of ROS/RNS, especially in live cells. Improvements have been made in sensing reactions, fluorophores, and bioavailability of probe molecules. In this review, we will not only summarize redox-related small-molecule fluorescent probes but also lay out the challenges of designing probes to help redox biologists independently evaluate the quality of reported small-molecule fluorescent probes, especially in the chemistry literature. We specifically highlight the advantages of reversibility in sensing reactions and its applications in ratiometric probe design for quantitative measurements in living cells. In addition, we compare the advantages and disadvantages of small-molecule probes and protein-based probes. The low physiological relevant concentrations of most ROS/RNS call for new sensing reactions with better selectivity, kinetics, and reversibility; fluorophores with high quantum yield, wide wavelength coverage, and Stokes shifts; and structural design with good aqueous solubility, membrane permeability, low protein interference, and organelle specificity. Antioxid. Redox Signal. 00, 000-000.

Bi-Directional Brillouin Optical Time Domain Analyzer System for Long Range Distributed Sensing

PubMed Central

Guo, Nan; Wang, Liang; Wang, Jie; Jin, Chao; Tam, Hwa-Yaw; Zhang, A. Ping; Lu, Chao

2016-01-01

We propose and experimentally demonstrate a novel scheme of bi-directional Brillouin time domain analyzer (BD-BOTDA) to extend the sensing range. By deploying two pump-probe pairs at two different wavelengths, the Brillouin frequency shift (BFS) distribution over each half of the whole fiber can be obtained with the simultaneous detection of Brillouin signals in both channels. Compared to the conventional unidirectional BOTDA system of the same sensing range, the proposed BD-BOTDA scheme enables distributed sensing with a performance level comparable to the conventional one with half of the sensing range and a spatial resolution of 2 m, while maintaining the Brillouin signal-to-noise ratio (SNR) and the BFS uncertainty. Based on this technique, we have achieved distributed temperature sensing with a measurement range of 81.9 km fiber at a spatial resolution of 2 m and BFS uncertainty of ~0.44 MHz without introducing any complicated components or schemes. PMID:27999250

Reflective photoluminescence fiber temperature probe based on the CdSe/ZnS quantum dot thin film

NASA Astrophysics Data System (ADS)

Wang, Helin; Yang, Aijun; Chen, Zhongshi; Geng, Yan

2014-08-01

A reflective fiber temperature sensor based on the optical temperature dependent characteristics of a quantum dots (QDs) thin film is developed by depositing the CdSe/ZnS core/shell quantum dots on the SiO2 glass substrates. As the temperature is changed from 30 to 200°C, the peak wavelengths of PL spectra from the sensing head increase linearly with the temperature, while the peak intensity and the full width at half maximum (FWHM) of PL spectra vary exponentially according to the specific physical law. Using the obtained temperature-dependent peak-wavelength shift, the average resolution of the designed fiber temperature sensor can reach 0.12 nm/°C, while it reaches 0.056 nm/°C according to the FWHM of PL spectrum.

Time-Resolved Luminescence Nanothermometry with Nitrogen-Vacancy Centers in Nanodiamonds

NASA Astrophysics Data System (ADS)

Tsai, Pei-Chang; Chen, Oliver Y.; Tzeng, Yan-Kai; Liu, Hsiou-Yuan; Hsu, Hsiang; Huang, Shaio-Chih; Chen, Jeson; Yee, Fu-Ghoul; Chang, Huan-Cheng; Chang, Ming-Shien

2016-05-01

Measuring thermal properties with nanoscale spatial resolution either at or far from equilibrium is gaining importance in many scientific and engineering applications. Although negatively charged nitrogen-vacancy (NV-) centers in diamond have recently emerged as promising nanometric temperature sensors, most previous measurements were performed under steady state conditions. Here we employ a three-point sampling method which not only enables real-time detection of temperature changes over +/-100 K with a sensitivity of 2 K/(Hz)1/2, but also allows the study of nanometer scale heat transfer with a temporal resolution of better than 1 μs with the use of a pump-probe-type experiment. In addition to temperature sensing, we further show that nanodiamonds conjugated with gold nanorods, as optically-activated dual-functional nanoheaters and nanothermometers, are useful for highly localized hyperthermia treatment. We experimentally demonstrated time-resolved fluorescence nanothermometry, and the validity of the measurements was verified with finite-element numerical simulations. The approaches provided here will be useful for probing dynamical thermal properties on nanodevices in operation.

In-pile Thermal Conductivity Characterization with Time Resolved Raman

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Xinwei; Hurley, David H.

The project is designed to achieve three objectives: (1) Develop a novel time resolved Raman technology for direct measurement of fuel and cladding thermal conductivity. (2) Validate and improve the technology development by measuring ceramic materials germane to the nuclear industry. (3) Conduct instrumentation development to integrate optical fiber into our sensing system for eventual in-pile measurement. We have developed three new techniques: time-domain differential Raman (TD-Raman), frequency-resolved Raman (FR-Raman), and energy transport state-resolved Raman (ET-Raman). The TD-Raman varies the laser heating time and does simultaneous Raman thermal probing, the FR-Raman probes the material’s thermal response under periodical laser heatingmore » of different frequencies, and the ET-Raman probes the thermal response under steady and pulsed laser heating. The measurement capacity of these techniques have been fully assessed and verified by measuring micro/nanoscale materials. All these techniques do not need the data of laser absorption and absolute material temperature rise, yet still be able to measure the thermal conductivity and thermal diffusivity with unprecedented accuracy. It is expected they will have broad applications for in-pile thermal characterization of nuclear materials based on pure optical heating and sensing.« less

Tracking Temperature-Dependent Relaxation Times of Ferritin Nanomagnets with a Wideband Quantum Spectrometer

NASA Astrophysics Data System (ADS)

Schäfer-Nolte, Eike; Schlipf, Lukas; Ternes, Markus; Reinhard, Friedemann; Kern, Klaus; Wrachtrup, Jörg

2014-11-01

We demonstrate the tracking of the spin dynamics of ensemble and individual magnetic ferritin proteins from cryogenic up to room temperature using the nitrogen-vacancy color center in diamond as a magnetic sensor. We employ different detection protocols to probe the influence of the ferritin nanomagnets on the longitudinal and transverse relaxation of the nitrogen-vacancy center, which enables magnetic sensing over a wide frequency range from Hz to GHz. The temperature dependence of the observed spectral features can be well understood by the thermally induced magnetization reversals of the ferritin and enables the determination of the anisotropy barrier of single ferritin molecules.

Probe-pin device for optical neurotransmitter sensing in the brain

NASA Astrophysics Data System (ADS)

Kim, Min Hyuck; Song, Kyo D.; Yoon, Hargsoon; Park, Yeonjoon; Choi, Sang H.; Lee, Dae-Sung; Shin, Kyu-Sik; Hwang, Hak-In; Lee, Uhn

2015-04-01

Development of an optical neurotransmitter sensing device using nano-plasmonic probes and a micro-spectrometer for real time monitoring of neural signals in the brain is underway. Clinical application of this device technology is to provide autonomous closed-loop feedback control to a deep brain stimulation (DBS) system and enhance the accuracy and efficacy of DBS treatment. By far, we have developed an implantable probe-pin device based on localized field enhancement of surface plasmonic resonance on a nanostructured sensing domain which can amplify neurochemical signals from evoked neural activity in the brain. In this paper, we will introduce the details of design and sensing performance of a proto-typed microspectrometer and nanostructured probing devices for real time measurement of neurotransmitter concentrations.

Colorimetric and Fluorescent Bimodal Ratiometric Probes for pH Sensing of Living Cells.

PubMed

Liu, Yuan-Yuan; Wu, Ming; Zhu, Li-Na; Feng, Xi-Zeng; Kong, De-Ming

2015-06-01

pH measurement is widely used in many fields. Ratiometric pH sensing is an important way to improve the detection accuracy. Herein, five water-soluble cationic porphyrin derivatives were synthesized and their optical property changes with pH value were investigated. Their pH-dependent assembly/disassembly behaviors caused significant changes in both absorption and fluorescence spectra, thus making them promising bimodal ratiometric probes for both colorimetric and fluorescent pH sensing. Different substituent identity and position confer these probes with different sensitive pH-sensing ranges, and the substituent position gives a larger effect. By selecting different porphyrins, different signal intensity ratios and different fluorescence excitation wavelengths, sensitive pH sensing can be achieved in the range of 2.1-8.0. Having demonstrated the excellent reversibility, good accuracy and low cytotoxicity of the probes, they were successfully applied in pH sensing inside living cells. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

MgF2 prism/rhodium/graphene: efficient refractive index sensing structure in optical domain

NASA Astrophysics Data System (ADS)

Mishra, Akhilesh Kumar; Mishra, Satyendra Kumar

2017-04-01

A theoretical study of a noble surface plasmon resonance (SPR) based sensing probe has been carried out. The sensing probe consists of a magnesium fluoride (MgF2) prism with its base coated with rarely used noble metal rhodium (Rh) and a bio-compatible layer of graphene. The refractive indices (RIs) of the sensing medium vary from 1.33 to 1.36 refractive index unit (RIU). The thickness of Rh and the number of graphene layers have been optimized for maximum sensitivity in a constraint set by the detection accuracy (DA). For the operating wavelength of 632 nm, the optimized sensing probe Rh (12 nm)/graphene (single layer) demonstrates sensitivity of ~259 degree/RIU with corresponding DA of ~0.32 degree-1 while for 532 nm of excitation, the optimized sensing probe Rh (12 nm)/graphene (three layer) exhibits sensitivity of ~240 degree/RIU and DA of ~0.27 degree-1.

MgF2 prism/rhodium/graphene: efficient refractive index sensing structure in optical domain.

PubMed

Mishra, Akhilesh Kumar; Mishra, Satyendra Kumar

2017-04-12

A theoretical study of a noble surface plasmon resonance (SPR) based sensing probe has been carried out. The sensing probe consists of a magnesium fluoride (MgF 2 ) prism with its base coated with rarely used noble metal rhodium (Rh) and a bio-compatible layer of graphene. The refractive indices (RIs) of the sensing medium vary from 1.33 to 1.36 refractive index unit (RIU). The thickness of Rh and the number of graphene layers have been optimized for maximum sensitivity in a constraint set by the detection accuracy (DA). For the operating wavelength of 632 nm, the optimized sensing probe Rh (12 nm)/graphene (single layer) demonstrates sensitivity of ~259 degree/RIU with corresponding DA of ~0.32 degree -1 while for 532 nm of excitation, the optimized sensing probe Rh (12 nm)/graphene (three layer) exhibits sensitivity of ~240 degree/RIU and DA of ~0.27 degree -1 .

Novel dual ligand co-functionalized fluorescent gold nanoclusters as a versatile probe for sensitive analysis of Hg(2+) and oxytetracycline.

PubMed

Xu, Shenghao; Li, Xiaolin; Mao, Yaning; Gao, Teng; Feng, Xiuying; Luo, Xiliang

2016-04-01

In this work, we present a direct one-step strategy for rapidly preparing dual ligand co-functionalized fluorescent Au nanoclusters (NCs) by using threonine (Thr) and 11-mercaptoundecanoic acid (MUA) as assorted reductants and capping agents in aqueous solution at room temperature. Fluorescence spectra, high-resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS), dynamic light scattering (DLS), and infrared (IR) spectroscopy were performed to demonstrate the optical properties and chemical composition of the as-prepared AuNCs. They possess many attractive features such as near-infrared emission (λem = 606 nm), a large Stoke's shift (>300 nm), high colloidal stability (pH, temperature, salt, and time stability), and water dispersibility. Subsequently, the as-prepared AuNCs were used as a versatile probe for "turn off" sensing of Hg(2+) based on aggregation-induced fluorescence quenching and for "turn-on" sensing of oxytetracycline (OTC). This assay provided good linearity ranging from 37.5 to 3750 nM for Hg(2+) and from 0.375 to 12.5 μM for OTC, with detection limits of 8.6 nM and 0.15 μM, respectively. Moreover, the practical application of this assay was further validated by detecting OTC in human serum samples.

A flavone-based turn-on fluorescent probe for intracellular cysteine/homocysteine sensing with high selectivity.

PubMed

Zhang, Jian; Lv, Yanlin; Zhang, Wei; Ding, Hui; Liu, Rongji; Zhao, Yongsheng; Zhang, Guangjin; Tian, Zhiyuan

2016-01-01

A new type of flavone-based fluorescent probe (DMAF) capable of cysteine (Cys)/homocysteine (Hcy) sensing with high selectivity over other amino acids was developed. Such type of probe undergoes Cys/Hcy-mediated cyclization reaction with the involvement of its aldehyde group, which suppresses of the photoinduced electron transfer (PET) process of the probe molecule and consequently leads to the enhancement of fluorescence emission upon excitation using visible light. The formation of product of the Cys/Hcy-mediated cyclization reaction was confirmed and the preliminary fluorescence imaging experiments revealed the biocompatibility of the as-prepared probe and validated its practicability for intracellular Cys/Hcy sensing. Copyright © 2015 Elsevier B.V. All rights reserved.

Single fiber temperature probe configuration using anti-Stokes luminescence from Cr:GdAlO3

NASA Astrophysics Data System (ADS)

Eldridge, Jeffrey I.

2018-06-01

Single-photon excitation of anti-Stokes-shifted emission from a thermographic phosphor allows operation of a luminescence decay-based single fiber temperature probe with negligible interference from background fiber-generated Raman scattering. While single fiber probe configurations for luminescence-based fiber optic thermometers offer advantages of simple design, compactness, and superior emission light collection efficiency, their effective use has been limited by interference from Raman scattering in the fiber probe and excitation delivery fiber that produces distortion of the luminescence decay that follows the excitation pulse. The near elimination of interference by background fiber-generated Raman scattering was demonstrated by incorporating a Cr-doped GdAlO3 (Cr:GdAlO3) thermographic phosphor as the sensing element at the end of a single fiber luminescence decay-based thermometer and detecting anti-Stokes-shifted luminescence centered at 542 or 593 nm produced by 695 nm excitation. Measurements were performed using both silica (up to 1150 °C) and single-crystal YAG (up to 1200 °C) fiber-based thermometers. Selection of emission detection centered at 542 nm greatly benefited the YAG fiber probe measurements by practically eliminating detection of otherwise significant luminescence from Cr3+ impurities in the YAG fiber. For both the silica and YAG fiber probes, the relative benefit of adopting single-photon excitation of anti-Stokes-shifted luminescence was evaluated by comparison with results obtained by conventional 532 nm excitation of Stokes-shifted luminescence.

First applications of the EXTASE thermal probe

NASA Astrophysics Data System (ADS)

Schröer, K.; Seiferlin, K.; Marczewski, W.; Gadomski, S.; Spohn, T.

2003-04-01

EXTASE is a spin-off project from the MUPUS (Rosetta Lander) thermal probe, both funded by DLR. The thermal probe will be tested in various environments and fields, e.g. in snow research, agriculture, permafrost, monitoring waste deposits and the heat released by decomposition, ground truth for remote sensing etc. The probe is a glass-fibre tube of 1cm diameter, about 32 cm long and carries of 16 sensors for measuring temperature profiles. Each of the sensors can also be heated for in situ measurements of the thermal diffusivity of the penetrated layers, from which we can derive the thermal conductivity. All necessary connections and the sensors itself are printed on a foil which is rolled and glued to the inner wall of the tube. This design results in the significant advantage that the measurements can be done in-situ. No excavation of material is required to measure the thermal conductivity, for instance. Presently we are concentrating on soil science and snow research.We made several measurements in different conditions with prototypes of the probe so far. Among other things, we measured soil temperatures together with meteorological boundary conditions in cooperation with the local Institute of Agrophysics in Lublin (Poland). The first measurements in snow under natural conditions were made on Svalbard (Spitzbergen) together with the Alfred-Wegener-Institute in Bremerhaven (Germany). First results of the measuring campaigns are shown.

Active Sensing System with In Situ Adjustable Sensor Morphology

PubMed Central

Nurzaman, Surya G.; Culha, Utku; Brodbeck, Luzius; Wang, Liyu; Iida, Fumiya

2013-01-01

Background Despite the widespread use of sensors in engineering systems like robots and automation systems, the common paradigm is to have fixed sensor morphology tailored to fulfill a specific application. On the other hand, robotic systems are expected to operate in ever more uncertain environments. In order to cope with the challenge, it is worthy of note that biological systems show the importance of suitable sensor morphology and active sensing capability to handle different kinds of sensing tasks with particular requirements. Methodology This paper presents a robotics active sensing system which is able to adjust its sensor morphology in situ in order to sense different physical quantities with desirable sensing characteristics. The approach taken is to use thermoplastic adhesive material, i.e. Hot Melt Adhesive (HMA). It will be shown that the thermoplastic and thermoadhesive nature of HMA enables the system to repeatedly fabricate, attach and detach mechanical structures with a variety of shape and size to the robot end effector for sensing purposes. Via active sensing capability, the robotic system utilizes the structure to physically probe an unknown target object with suitable motion and transduce the arising physical stimuli into information usable by a camera as its only built-in sensor. Conclusions/Significance The efficacy of the proposed system is verified based on two results. Firstly, it is confirmed that suitable sensor morphology and active sensing capability enables the system to sense different physical quantities, i.e. softness and temperature, with desirable sensing characteristics. Secondly, given tasks of discriminating two visually indistinguishable objects with respect to softness and temperature, it is confirmed that the proposed robotic system is able to autonomously accomplish them. The way the results motivate new research directions which focus on in situ adjustment of sensor morphology will also be discussed. PMID:24416094

Thin Cu film resistivity using four probe techniques: Effect of film thickness and geometrical shapes

NASA Astrophysics Data System (ADS)

Choudhary, Sumita; Narula, Rahul; Gangopadhyay, Subhashis

2018-05-01

Precise measurement of electrical sheet resistance and resistivity of metallic thin Cu films may play a significant role in temperature sensing by means of resistivity changes which can further act as a safety measure of various electronic devices during their operation. Four point probes resistivity measurement is a useful approach as it successfully excludes the contact resistance between the probes and film surface of the sample. Although, the resistivity of bulk samples at a particular temperature mostly depends on its materialistic property, however, it may significantly differ in the case of thin films, where the shape and thickness of the sample can significantly influence on it. Depending on the ratio of the film thickness to probe spacing, samples are usually classified in two segments such as (i) thick films or (ii) thin films. Accordingly, the geometric correction factors G can be related to the sample resistivity r, which has been calculated here for thin Cu films of thickness up to few 100 nm. In this study, various rectangular shapes of thin Cu films have been used to determine the shape induced geometric correction factors G. An expressions for G have been obtained as a function of film thickness t versus the probe spacing s. Using these expressions, the correction factors have been plotted separately for each cases as a function of (a) film thickness for fixed linear probe spacing and (b) probe distance from the edge of the film surface for particular thickness. Finally, we compare the experimental results of thin Cu films of various rectangular geometries with the theoretical reported results.

All-optical nanoscale thermometry with silicon-vacancy centers in diamond

NASA Astrophysics Data System (ADS)

Nguyen, Christian T.; Evans, Ruffin E.; Sipahigil, Alp; Bhaskar, Mihir K.; Sukachev, Denis D.; Agafonov, Viatcheslav N.; Davydov, Valery A.; Kulikova, Liudmila F.; Jelezko, Fedor; Lukin, Mikhail D.

2018-05-01

We demonstrate an all-optical thermometer based on an ensemble of silicon-vacancy centers (SiVs) in diamond by utilizing the sensitivity of the zero-phonon line wavelength to temperature, Δλ/ΔT =0.0124 (2 ) nm K-1 [6.8(1) GHz K-1]. Using SiVs in bulk diamond, we achieve 70 mK precision at room temperature with a temperature uncertainty σT=360 mK/√{H z } . Finally, we use SiVs in 200 nm nanodiamonds as local temperature probes with 521 mK/ √{H z } uncertainty and achieve sub-Kelvin precision. These properties deviate by less than 1% between nanodiamonds, enabling calibration-free thermometry for sensing and control of complex nanoscale systems.

Using passive fiber-optic distributed temperature sensing to estimate soil water content at a discontinuous permafrost site

NASA Astrophysics Data System (ADS)

Wagner, A. M.; Lindsey, N.; Ajo Franklin, J. B.; Gelvin, A.; Saari, S.; Ekblaw, I.; Ulrich, C.; Dou, S.; James, S. R.; Martin, E. R.; Freifeld, B. M.; Bjella, K.; Daley, T. M.

2016-12-01

We present preliminary results from an experimental study targeting the use of passive fiber-optic distributed temperature sensing (DTS) in a variety of geometries to estimate moisture content evolution in a dynamic permafrost system. A 4 km continuous 2D array of multi-component fiber optic cable (6 SM/6 MM) was buried at the Fairbanks Permafrost Experiment Station to investigate the possibility of using fiber optic distributed sensing as an early detection system for permafrost thaw. A heating experiment using 120 60 Watt heaters was conducted in a 140 m2 area to artificially thaw the topmost section of permafrost. The soils at the site are primarily silt but some disturbed areas include backfilled gravel to depths of approximately 1.0 m. Where permafrost exists, the depth to permafrost ranges from 1.5 to approximately 5 m. The experiment was also used to spatially estimate soil water content distribution throughout the fiber optic array. The horizontal fiber optic cable was buried at depths between 10 and 20 cm. Soil temperatures were monitored with a DTS system at 25 cm increments along the length of the fiber. At five locations, soil water content time-domain reflectometer (TDR) probes were also installed at two depths, in line with the fiber optic cable and 15 to 25 cm below the cable. The moisture content along the fiber optic array was estimated using diurnal effects from the dual depth temperature measurements. In addition to the horizontally installed fiber optic cable, vertical lines of fiber optic cable were also installed inside and outside the heater plot to a depth of 10 m in small diameter (2 cm) boreholes. These arrays were installed in conjunction with thermistor strings and are used to monitor the thawing process and to cross correlate with soil temperatures at the depth of the TDR probes. Results will be presented from the initiation of the artificial thawing through subsequent freeze-up. A comparison of the DTS measured temperatures and thermistors in vertically installed PVC pipes will also be shown. Initial results from a thermal model of the artificial heating experiment and the model's correlation to the actual soil temperature measurements will also be presented. These results show the possibility of using fiber optic cable to measure moisture contents along a longer array with only limited control points.

Research study of pressure instrumentation

NASA Technical Reports Server (NTRS)

Hoogenboom, L.; Hull-Allen, G.

1984-01-01

To obtain a more vibration resistant pressure sensor for use on the Space Shuttle Main Engine, a proximity probe based, diaphragm type pressure sensor breadboard was developed. A fiber optic proximity probe was selected as the sensor. In combination with existing electronics, a thermal stability evaluation of the entire probe system was made. Based upon the results, a breadboard design of the pressure sensor and electronics was made and fabricated. A brief series of functional experiments was made with the breadboard to calibrate, thermally compensate, and linearize its response. In these experiments, the performance obtained in the temperature range of -320 F (liquid N2) to +200 F was comparable to that of the strain gage based sensor presently in use on the engine. In tests at NASA-Marshall Space Flight Center (MSFC), after some time at or near liquid nitrogen temperatures, the sensor output varied over the entire output range. These large spurious signals were attributed to condensation of air in the sensing gap. In the next phase of development of this sensor, an evaluation of fabrication techniques toward greater thermal and mechanical stability of the fiber probe assembly must be made. In addition to this, a positive optics to metal seal must be developed to withstand the pressure that would result from a diaphragm failure.

Novel fiber optic sensor probe with a pair of highly reflected connectors and a vessel of water absorption material for water leak detection.

PubMed

Cho, Tae-Sik; Choi, Ki-Sun; Seo, Dae-Cheol; Kwon, Il-Bum; Lee, Jung-Ryul

2012-01-01

The use of a fiber optic quasi-distributed sensing technique for detecting the location and severity of water leakage is suggested. A novel fiber optic sensor probe is devised with a vessel of water absorption material called as water combination soil (WCS) located between two highly reflected connectors: one is a reference connector and the other is a sensing connector. In this study, the sensing output is calculated from the reflected light signals of the two connectors. The first reflected light signal is a reference and the second is a sensing signal which is attenuated by the optical fiber bending loss due to the WCS expansion absorbing water. Also, the bending loss of each sensor probe is determined by referring to the total number of sensor probes and the total power budget of an entire system. We have investigated several probe characteristics to show the design feasibility of the novel fiber sensor probe. The effects of vessel sizes of the probes on the water detection sensitivity are studied. The largest vessel probe provides the highest sensitivity of 0.267 dB/mL, while the smallest shows relatively low sensitivity of 0.067 dB/mL, and unstable response. The sensor probe with a high output value provides a high sensitivity with various detection levels while the number of total installable sensor probes decreases.

Temperature sensor with improved thermal barrier and gas seal between the probe and housing

DOEpatents

O'Connell, David Peter; Sumner, Randall Christian

1998-01-01

A temperature sensor comprising: a hollow tube with a first end and a second end, wherein the second end is closed sealing a cavity within the tube from an environment outside of the tube and wherein the first end has an exterior cylindrical surface; a temperature responsive sensing element within the tube proximate to the second end; a glass cylinder having an inner cylindrical surface in sealing engagement with the exterior cylindrical surface of the first end of the tube; and a sensor housing having an inner cylindrical cavity bounded by an inner cylindrical wall, wherein an outer cylindrical surface of the glass cylinder is sealingly engaged with the inner cylindrical wall.

A cell-surface-anchored ratiometric fluorescent probe for extracellular pH sensing.

PubMed

Ke, Guoliang; Zhu, Zhi; Wang, Wei; Zou, Yuan; Guan, Zhichao; Jia, Shasha; Zhang, Huimin; Wu, Xuemeng; Yang, Chaoyong James

2014-09-10

Accurate sensing of the extracellular pH is a very important yet challenging task in biological and clinical applications. This paper describes the development of an amphiphilic lipid-DNA molecule as a simple yet useful cell-surface-anchored ratiometric fluorescent probe for extracellular pH sensing. The lipid-DNA probe, which consists of a hydrophobic diacyllipid tail and a hydrophilic DNA strand, is modified with two fluorescent dyes; one is pH-sensitive as pH indicator and the other is pH-insensitive as an internal reference. The lipid-DNA probe showed sensitive and reversible response to pH change in the range of 6.0-8.0, which is suitable for most extracellular studies. In addition, based on simple hydrophobic interactions with the cell membrane, the lipid-DNA probe can be easily anchored on the cell surface with negligible cytotoxicity, excellent stability, and unique ratiometric readout, thus ensuring its accurate sensing of extracellular pH. Finally, this lipid-DNA-based ratiometric pH indicator was successfully used for extracellular pH sensing of cells in 3D culture environment, demonstrating the potential applications of the sensor in biological and medical studies.

The Galileo Probe: How it Has Changed Our Understanding of Jupiter

NASA Technical Reports Server (NTRS)

Young, Richard E.

2003-01-01

The Galileo Mission to Jupiter, which arrived in December of 1995, provided the first study by an orbiter, and the first in-situ sampling via an entry probe, of an outer planet atmosphere. The rationale for an entry probe is that, even from an orbiter, remote sensing of the jovian atmosphere could not adequately retrieve the information desired. This paper provides a current summary of the most significant aspects of the data returned from the Galileo entry probe. As a result of the probe measurements, there has been a reassessment of our understanding of outer planet formation and evolution of the solar system. The primary scientific objective of the Galileo probe was to determine the composition of the jovian atmosphere, which from remote sensing remained either very uncertain, or completely unknown, with respect to several key elements. The probe found that the global He mass fraction is. significantly above the value reported from the Voyager Jupiter flybys but is slightly below the protosolar value, implying that there has been some settling of He to the deep jovian interior. The probe He measurements have also led to a reevaluation of the Voyager He mass fraction for Saturn, which is now determined to be much closer to that of Jupiter. The elements C, N, S, Ar, Kr, Xe were all found to have global abundances approximately 3 times their respective solar abundances. This result has raised a number of fundamental issues with regard to properties of planetesimals and the solar nebula at the time of giant planet formation. Ne, on the other hand, was found to be highly depleted, probably as the result of it being carried along with helium as helium settles towards the deep interior. The global abundance of O was not obtained by the probe because of the influence of local processes at the probe entry site (PES), processes which depleted condensible species, in this case H2O, well below condensation levels. Other condensible species, namely NH3 and H2S, were similarly affected but attained their deep equilibrium mixing ratios before the maximum depth sampled by the probe. Processes that might be capable of producing such effects on the condensibles are still under investigation. Measured isotopic ratios of noble gases and other heavy elements are solar, and (D + (Sup 3)He)/H is the same to within measurement uncertainties as in the local interstellar medium. No thick clouds were detected, and in particular no significant water cloud, but the PES location clearly affected the probe measurements of clouds. In fact, the probe data must be understood in the context of the location of the PES, which was within what is termed a 5 micron hot spot, a local clearing in the clouds that is bright near the 5 microns spectral region. The thermal structure at the PES was determined from approximately 1000 km above the 1 bar pressure level (10(exp -9 bars)) to 132 km 1 bar (22bars). The probe showed the atmosphere to have a generally sub-adiabatic temperature gradient (static stability) of = 0.1 K/km to as deep as the probe made measurements. In the upper atmosphere the probe derived a maximum positive vertical temperature gradient of approximately 5 K/km, and maximum temperature of = 900 K. The energy sources producing the warm upper atmosphere have yet to be completely identified. At first glance, Doppler tracking of the probe indicates that the long observed cloud level zonal winds extend to levels at least as deep as the probe made measurements. Zonal wind increases from = 80 m/s at pressures less than a bar to about 180 m/s near 5 bars, and remains approximately constant with depth thereafter. However, there is a question as to whether the winds measured from probe tracking are representative of the general wind field, or are considerably influenced by localized winds associated with the PES.

Immobilization of human papillomavirus DNA probe for surface plasmon resonance imaging

NASA Astrophysics Data System (ADS)

Chong, Xinyuan; Ji, Yanhong; Ma, Suihua; Liu, Le; Liu, Zhiyi; Li, Yao; He, Yonghong; Guo, Jihua

2009-08-01

Human papillomavirus (HPV) is a kind of double-stranded DNA virus whose subspecies have diversity. Near 40 kinds of subspecies can invade reproductive organ and cause some high risk disease, such as cervical carcinoma. In order to detect the type of the subspecies of the HPV DNA, we used the parallel scan spectral surface plasmon resonance (SPR) imaging technique, which is a novel type of two- dimensional bio-sensing method based on surface plasmon resonance and is proposed in our previous work, to study the immobilization of the HPV DNA probes on the gold film. In the experiment, four kinds of the subspecies of the HPV DNA (HPV16, HPV18, HPV31, HPV58) probes are fixed on one gold film, and incubate in the constant temperature condition to get a HPV DNA probe microarray. We use the parallel scan spectral SPR imaging system to detect the reflective indices of the HPV DNA subspecies probes. The benefits of this new approach are high sensitive, label-free, strong specificity and high through-put.

Program for an improved hypersonic temperature-sensing probe

NASA Technical Reports Server (NTRS)

Reilly, Richard J.

1993-01-01

Under a NASA Dryden-sponsored contract in the mid 1960s, temperatures of up to 2200 C were successfully measured using a fluid oscillator. The current program, although limited in scope, explores the problem areas which must be solved if this technique is to be extended to 10,000 R. The potential for measuring extremely high temperatures, using fluid oscillator techniques, stems from the fact that the measuring element is the fluid itself. The containing structure of the oscillator need not be brought to equilibrium temperature with with the fluid for temperature measurement, provided that a suitable calibration can be arranged. This program concentrated on review of high-temperature material developments since the original program was completed. Other areas of limited study included related pressure instrumentation requirements, dissociation, rarefied gas effects, and analysis of sensor time response.

Performance of Surface-Mount Ceramic and Solid Tantalum Capacitors for Cryogenic Applications

NASA Technical Reports Server (NTRS)

Patterson, Richard L.; MacDonald, Thomas L.; Hammoud, Ahmad; Gerber, Scott

1998-01-01

Low temperature electronics are of great interest for space exploration programs. These include missions to the outer planets, earth-orbiting and deep-space probes, remote-sensing and communication satellites. Terrestrial applications would also benefit from the availability of low temperature electronics. Power components capable of low temperature operation would, thus, enhance the technologies needed for the development of advanced power systems suitable for use in harsh environments. In this work, ceramic and solid tantalum capacitors were evaluated in terms of their dielectric properties as a function of temperature and at various frequencies. The surface-mount devices were characterized in terms of their capacitance stability and dissipation factor in the frequency range of 50 Hz to 100 kHz at temperatures ranging from room temperature (20 deg. C) to about liquid nitrogen temperature (-190 deg. C). The results are discussed and conclusions made concerning the suitability of the capacitors investigated for low temperature applications.

Synthesis of improved upconversion nanoparticles as ultrasensitive fluorescence probe for mycotoxins.

PubMed

Chen, Quansheng; Hu, Weiwei; Sun, Cuicui; Li, Huanhuan; Ouyang, Qin

2016-09-28

Rare earth-doped upconversion nanoparticles (UCNPs) have promising potentials in biodetection due to their unique frequency upconverting capability and high detection sensitivity. This paper reports an improved UCNPs-based fluorescence probe for dual-sensing of Aflatoxin B1 (AFB1) and Deoxynivalenol (DON) using a magnetism-induced separation and the specific formation of antibody-targets complex. Herein, the improved UCNPs, which were namely NaYF4:Yb/Ho/Gd and NaYF4:Yb/Tm/Gd, were systematically studied based on the optimization of reaction time, temperature and the concentration of dopant ions with simultaneous phase and size controlled NaYF4 nanoparticles; and the targets were detected using the pattern of competitive combination assay. Under an optimized condition, the advanced fluorescent probes revealed stronger fluorescent properties, broader biological applications and better storage stabilities compared to traditional UCNPs-based ones; and ultrasensitive determinations of AFB1 and DON were achieved under a wide sensing range of 0.001-0.1 ng ml(-1) with the limit of detection (LOD) of 0.001 ng ml(-1). Additionally, the applicability of the improved nanosensor for the detection of mycotoxins was also confirmed in adulterated oil samples. Copyright © 2016 Elsevier B.V. All rights reserved.

Live Cells as Dynamic Laboratories: Time Lapse Raman Spectral Microscopy of Nanoparticles with Both IgE Targeting and pH-Sensing Functions

DOE PAGES

Nowak-Lovato, Kristy L.; Rector, Kirk D.

2012-01-01

Tmore » his review captures the use of live cells as dynamic microlaboratories through implementation of labeled nanoparticles (nanosensors) that have both sensing and targeting functions. he addition of 2,4-ε-dinitrophenol-L-lysine (DNP) as a FcεRI targeting ligand and 4-mercaptopyridine (4-MPy) as a pH-sensing ligand enables spatial and temporal monitoring of FcεRI receptors and their pH environment within the endocytic pathway. o ensure reliability, the sensor is calibrated in vivo using the ionophore nigericin and standard buffer solutions to equilibrate the external [ H + ] concentration with that of the cell compartments. his review highlights the nanosensors, ability to traffic and respond to pH of receptor-bound nanosensors (1) at physiological temperature ( 37 ° C ) versus room temperature ( 25 ° C ) , (2) after pharmacological treatment with bafilomycin, an H + APase pump inhibitor, or amiloride, an inhibitor of Na + / H + exchange, and (3) in response to both temperature and pharmacological treatment. Whole-cell, time lapse images are demonstrated to show the ability to transform live cells into dynamic laboratories to monitor temporal and spatial endosomal pH. he versatility of these probes shows promise for future applications relevant to intracellular trafficking and intelligent drug design.« less

Time-resolved fluorescence sensing of pesticides chlorpyrifos, crotoxyphos and endosulfan by the luminescent Eu(III)-8-allyl-3-carboxycoumarin probe

NASA Astrophysics Data System (ADS)

Azab, Hassan A.; Khairy, Gasser M.; Kamel, Rasha M.

2015-09-01

This work describes the application of time resolved fluorescence in microtiter plates for investigating the interactions of europium-allyl-3-carboxycoumarin with pesticides chlorpyrifos, endosulfan and crotoxyphos. Stern-Volmer studies at different temperatures for chlorpyrifos and crotoxyphos shows dynamic and static quenching mechanisms respectively. Direct methods for the determination of the pesticides under investigation have been developed using the luminescence variations of the probe in solution. The detection limits are 6.53, 0.004, 3.72 μmol/L for chlorpyrifos, endosulfan, and crotoxyphos, respectively. The binding constants and thermodynamic parameters of the pesticides with probe were evaluated. A thermodynamic analysis showed that the reaction is spontaneous with negative ΔG. Effect of some relevant interferents on the detection of pesticides has been investigated. The new method was applied to the determination of the pesticides in different types of water samples (tap, mineral, and waste water).

Exploring the recognized bio-mimicry materials for gas sensing.

PubMed

Wu, T Z; Lo, Y R; Chan, E C

2001-12-01

This study was undertaken to synthesize peptides that are partially similar to the binding sites of human olfactory receptor protein. First, a putative 3-D model structure of human olfactory receptor protein (P30953) was modeled using a molecular simulation method. The computer docking simulation was then performed to determine the most plausible binding sites between the model structure and target gases, trimethylamine, ammonia, acetic acid, and o-xylene. According to the simulation result, a series of polypeptide sequences, horp61 for TMA, horp103 for o-xylene, horp109 for ammonia, and horp193 for acetic acid as recognized molecules were designed for gas sensing purposes. Preparing these peptides as corresponding gas sensing probes, the results showed a high relative sensitivity response of 6.7 for TMA (probe horp61), 5.1 for o-xylene (probe horp103), 11 for ammonia (probe horp109), and 28 for acetic acid (probe horp193), respectively. These results indicate that peptide mimicking of binding domain on olfactory receptor opens a new window and offers a novel strategy for the further development of recognized materials for gas sensing.

NEUTRONIC REACTOR CORE INSTRUMENT

DOEpatents

Mims, L.S.

1961-08-22

A multi-purpose instrument for measuring neutron flux, coolant flow rate, and coolant temperature in a nuclear reactor is described. The device consists essentially of a hollow thimble containing a heat conducting element protruding from the inner wall, the element containing on its innermost end an amount of fissionsble materinl to function as a heat source when subjected to neutron flux irradiation. Thermocouple type temperature sensing means are placed on the heat conducting element adjacent the fissionable material and at a point spaced therefrom, and at a point on the thimble which is in contact with the coolant fluid. The temperature differentials measured between the thermocouples are determinative of the neutron flux, coolant flow, and temperature being measured. The device may be utilized as a probe or may be incorporated in a reactor core. (AE C)

Measuring Fluxes Of Heat To A Plasma-Arc Anode

NASA Technical Reports Server (NTRS)

Sankovic, John M.; Menart, James A.; Pfender, Emil; Heberlein, Joachim

1995-01-01

Three probes constructed to provide measurements indicative of conductive, convective, and radiative transfer of heat from free-burning plasma arc to water-cooled copper anode used in generating arc. Each probe consists mainly of copper body with two thermocouples embedded at locations 4 mm apart along length. Thermocouples provide measure of rate of conduction of heat along probe and transfers of heat from plasma to sensing surface at tip of probe. Probes identical except sensing surface of one uncoated and other two coated with different materials to make them sensitive to different components of overall flux of heat.

Fiber Bragg Grating Array as a Quasi Distributed Temperature Sensor for Furnace Boiler Applications

NASA Astrophysics Data System (ADS)

Reddy, P. Saidi; Prasad, R. L. N. Sai; Sengupta, D.; Shankar, M. Sai; Srimannarayana, K.; Kishore, P.; Rao, P. Vengal

2011-10-01

This paper presents the experimental work on distributed temperature sensing making use of Fiber Bragg grating (FBG) array sensor for possible applications in the monitoring of temperature profile in high temperature boilers. A special sensor has been designed for this purpose which consists of four FBGs (of wavelengths λB1 = 1547.28 nm, λB2 = 1555.72 nm, λB3 = 1550.84 nm, λB4 = 1545.92 nm) written in hydrogen loaded fiber in line with a spacing of 15 cm between them. All the FBGs are encapsulated inside a stainless steel tube for avoiding micro cracks using rigid probe technique. The spatial distribution of temperature profile inside a prototype boiler has been measured experimentally both in horizontal and vertical directions employing the above sensor and the results are presented.

The construction, characterization, Hg(II)-sensing and removal behavior of magnetic core-shell nanospheres loaded with fluorescence "Off-On" probe

NASA Astrophysics Data System (ADS)

Tan, Jun; Wei, Xiaoyan; Chen, Jie; Sun, Ping; Ouyang, Yuxia; Fan, Juhong; Liu, Rui

2014-12-01

The present paper constructed and discussed core-shell structured nanospheres grafted with rhodamine based probe for Hg(II) sensing and removal. Electron microscopy images, XRD curves, thermogravimetric analysis and N2 adsorption/desorption isotherms were used to identify the core-shell structure. The inner core consisted of superparamagnetic Fe3O4 nanoparticles, which made the nanocomposite magnetically removable. The outer shell was constructed with silica molecular sieve which provided large surface area and ordered tunnels for the sensing probe, accelerating analyte adsorption and transportation. The rhodamine based sensing probe emission increased with the increasing Hg(II) concentration, showing emission "Off-On" effect, which could be explained by the structural transformation from a non-emissive one to a highly emissive one. The influence from various metal ions and pH values was also investigated, which suggested this structural transformation could only be triggered by Hg(II), showing high selectivity and linear response. The Hg(II) sensing nanocomposite could be regenerated after usage. The response time was slightly compromised and could be further improved.

A Novel Water-soluble Ratiometric Fluorescent Probe Based on FRET for Sensing Lysosomal pH.

PubMed

Song, Guang-Jie; Bai, Su-Yun; Luo, Jing; Cao, Xiao-Qun; Zhao, Bao-Xiang

2016-11-01

A new ratiometric fluorescent probe based on Förster resonance energy transfer (FRET) for sensing lysosomal pH has been developed. The probe (RMPM) was composed of imidazo[1,5-α]pyridine quaternary ammonium salt fluorophore as the FRET donor and the rhodamine moiety as the FRET acceptor. It's the first time to report that imidazo[1,5-α]pyridine quaternary ammonium salt acts as the FRET donor. The ratio of fluorescence intensity of the probe at two wavelengths (I 424 /I 581 ) changed significantly and responded linearly toward minor pH changes in the range of 5.4-6.6. It should be noted that it's rare to report that a ratiometric pH probe could detect so weak acidic pH with pKa = 6.31. In addition, probe RMPM exhibited excellent water-solubility, fast-response, all-right selectivity and brilliant reversibility. Moreover, RMPM has been successfully applied to sensing lysosomal pH in HeLa cells and has low cytotoxicity.

Automated measurement system employing eddy currents to adjust probe position and determine metal hardness

DOEpatents

Prince, J.M.; Dodson, M.G.; Lechelt, W.M.

1989-07-18

A system for measuring the hardness of cartridge cases employs an eddy current probe for inducing and sensing eddy currents in each cartridge case. A first component of the sensed signal is utilized in a closed loop system for accurately positioning the probe relative to the cartridge case both in the lift off direction and in the tangential direction, and a second component of the sensed signal is employed as a measure of the hardness. The positioning and measurement are carried out under closed loop microprocessor control facilitating hardness testing on a production line basis. 14 figs.

Automated measurement system employing eddy currents to adjust probe position and determine metal hardness

DOEpatents

Prince, James M.; Dodson, Michael G.; Lechelt, Wayne M.

1989-01-01

A system for measuring the hardness of cartridge cases employs an eddy current probe for inducing and sensing eddy currents in each cartridge case. A first component of the sensed signal is utilized in a closed loop system for accurately positioning the probe relative to the cartridge case both in the lift off direction and in the tangential direction, and a second component of the sensed signal is employed as a measure of the hardness. The positioning and measurement are carried out under closed loop microprocessor control facilitating hardness testing on a production line basis.

The Hera Entry Probe Mission to Saturn, an ESA M-class mission proposal

NASA Astrophysics Data System (ADS)

Mousis, O.; Atkinson, D. H.; Spilker, T.; Venkatapathy, E.; Poncy, J.; Coustenis, A.; Reh, K.

2015-10-01

A fundamental goal of solar system exploration is to understand the origin of the solar system, the initial stages, conditions, and processes by which the solar system formed, how the formation process was initiated, and the nature of the interstellar seed material from which the solar system was born. Key to understanding solar system formation and subsequent dynamical and chemical evolution is the origin and evolution of the giant planets and their atmospheres. Additionally, the atmospheres of the giant planets serve as laboratories to better understand the atmospheric chemistries, dynamics, processes, and climates on all planets in the solar system including Earth, offer a context and provide a ground truth for exoplanets and exoplanetary systems,and have long been thought to play a critical role in the development of potentially habitable planetary systems. Remote sensing observations are limited when used to study the bulk atmospheric composition of the giant planets of our solar system. A remarkable example of the value of in situ probe measurements is illustrated by the exploration of Jupiter, where key measurements such as noble gases abundances and the precise measurement of the helium mixing ratio have only been made available through in situ measurements by the Galileo probe. Representing the only method providing ground-truth to connect the remote sensing inferences with physical reality, in situ measurements have only been accomplished twice in the history of outer solar system exploration, via the Galileo probe for Jupiter and the Huygens probe for Titan. In situ measurements provide access to atmospheric regions that are beyond the reach of remote sensing, enabling the dynamical, chemical and aerosol-forming processes at work from the thermosphere to the troposphere below the cloud decks to be studied. A proposal for a Saturn entry probe mission named Hera was recently submitted to the European Space Agency Medium Class mission announcement of opportunity. Hera comprises a single entry probe carried by a flyby spacecraft that will also act as a relay station to receive the probe science telemetry for recording and later transmission to Earth. A solar powered mission, Hera will take approximately 8 years to reach Saturn and will descend under a sequence of parachutes to depths of at least 10 bars in approximately 75 minutes. The Hera probe will carry a Mass Spectrometer to measure the composition of Saturn's atmosphere, an Atmospheric Structure Instrument to measure atmospheric pressures and temperatures, and a Doppler Wind Experiment to measure the dynamics of Saturn's atmosphere. Other possible instruments in the Hera scientific payload include a Net Flux Radiometer to measure the energy balance of the Saturn atmosphere and a Nephelometer to measure cloud locations and densities. In the context of giant planet science provided by the Galileo, Juno, and Cassini missions to Jupiter and Saturn, the Hera Saturn probe will provide critical measurements of composition, structure, and processes that are not accessible by remote sensing. The results of Hera will help test competing theories of solar system and giant planet origin, chemical, and dynamical evolution.

Magnetic field sensing with nitrogen-vacancy color centers in diamond

NASA Astrophysics Data System (ADS)

Pham, Linh My

In recent years, the nitrogen-vacancy (NV) center has emerged as a promising magnetic sensor capable of measuring magnetic fields with high sensitivity and spatial resolution under ambient conditions. This combination of characteristics allows NV magnetometers to probe magnetic structures and systems that were previously inaccessible with alternative magnetic sensing technologies This dissertation presents and discusses a number of the initial efforts to demonstrate and improve NV magnetometry. In particular, a wide-field CCD based NV magnetic field imager capable of micron-scale spatial resolution is demonstrated; and magnetic field alignment, preferential NV orientation, and multipulse dynamical decoupling techniques are explored for enhancing magnetic sensitivity. The further application of dynamical decoupling control sequences as a spectral probe to extract information about the dynamics of the NV spin environment is also discussed; such information may be useful for determining optimal diamond sample parameters for different applications. Finally, several proposed and recently demonstrated applications which take advantage of NV magnetometers' sensitivity and spatial resolution at room temperature are presented, with particular focus on bio-magnetic field imaging.

Multifunctional semiconductor micro-Hall devices for magnetic, electric, and photo-detection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gilbertson, A. M.; Cohen, L. F.; Sadeghi, Hatef

2015-12-07

We report the real-space voltage response of InSb/AlInSb micro-Hall devices to local photo-excitation, electric, and magnetic fields at room temperature using scanning probe microscopy. We show that the ultrafast generation of localised photocarriers results in conductance perturbations analogous to those produced by local electric fields. Experimental results are in good agreement with tight-binding transport calculations in the diffusive regime. The magnetic, photo, and charge sensitivity of a 2 μm wide probe are evaluated at a 10 μA bias current in the Johnson noise limit (valid at measurement frequencies > 10 kHz) to be, respectively, 500 nT/√Hz; 20 pW/√Hz (λ = 635 nm) comparable to commercial photoconductive detectors;more » and 0.05 e/√Hz comparable to that of single electron transistors. These results demonstrate the remarkably versatile sensing attributes of simple semiconductor micro-Hall devices that can be applied to a host of imaging and sensing applications.« less

Hybrid spin-microcantilever sensor for environmental, chemical, and biological detection

NASA Astrophysics Data System (ADS)

Wu, Wen-Hao; Zhu, Ka-Di

2015-01-01

Nowadays hybrid spin-micro/nanomechanical systems are being actively explored for potential quantum sensing applications. In combination with the pump-probe technique or the spin resonance spectrum, we theoretically propose a realistic, feasible, and an exact way to measure the cantilever frequency in a hybrid spin-micromechanical cantilever system which has a strong coherent coupling of a single nitrogen vacancy center in the single-crystal diamond cantilever with the microcantilever. The probe absorption spectrum which exhibits new features such as mechanically induced three-photon resonance and ac Stark effect is obtained. Simultaneously, we further develop this hybrid spin-micromechanical system to be an ultrasensitive mass sensor, which can be operated at 300 K with a mass responsivity 0.137 Hz ag-1, for accurate sensing of gaseous or aqueous environments, chemical vapors, and biomolecules. And the best performance on the minimum detectable mass can be 28.7 zg in vacuum. Finally, we illustrate an in situ measurement to detect Angiopoietin-1, a marker of tumor angiogenesis, accurately with this hybrid microcantilever at room temperature.

Sensitivity Limits of Rydberg Atom-Based Radio Frequency Electric Field Sensing

NASA Astrophysics Data System (ADS)

Jahangiri, Akbar J.; Kumar, Santosh; Kuebler, Harald; Fan, Haoquan; Shaffer, James P.

2017-04-01

We present progress on Rydberg atom-based RF electric field sensing using Rydberg state electromagnetically induced transparency (EIT) in room temperature atomic vapor cells. In recent experiments on homodyne detection with a Mach-Zehnder interferometer and frequency modulation spectroscopy with active control of residual amplitude modulation we determined that photon shot noise on the probe laser detector limits the sensitivity. Another factor that limits the accuracy is residual Doppler broadening due to the wave-vector mismatch between the coupling and the probe lasers. The sensor as limited by project noise can be orders of magnitude better. A multi-photon scheme is presented that can eliminate the residual Doppler effect by matching the wave-vectors of three lasers and reduce the photon shot noise limit by correctly choosing the Rabi frequencies of the first two steps of the EIT scheme. Using density matrix calculations, we predict that the three-photon approach can improve the detection sensitivity to below 200 nV cm-1 Hz- 1 / 2 and expand the Autler-Townes regime which improves the accuracy. This work is supported by DARPA and the NRO.

Temperature sensor with improved thermal barrier and gas seal between the probe and housing

DOEpatents

O`Connell, D.P.; Sumner, R.C.

1998-04-28

A temperature sensor is disclosed comprising: a hollow tube with a first end and a second end, wherein the second end is closed sealing a cavity within the tube from an environment outside of the tube and wherein the first end has an exterior cylindrical surface; a temperature responsive sensing element within the tube proximate to the second end; a glass cylinder having an inner cylindrical surface in sealing engagement with the exterior cylindrical surface of the first end of the tube; and a sensor housing having an inner cylindrical cavity bounded by an inner cylindrical wall, wherein an outer cylindrical surface of the glass cylinder is sealingly engaged with the inner cylindrical wall. 1 fig.

Effect of structure on sensing performance of a target induced signaling probe shifting DNA-based (TISPS-DNA) sensor.

PubMed

Yu, Xiang; Yu, Zhigang; Li, Fengqin; Xu, Yanmei; He, Xunjun; Xu, Lan; Shi, Wenbing; Zhang, Guiling; Yan, Hong

2017-05-15

A type of "signal on" displacement-based sensors named target induced signaling probe shifting DNA-based (TISPS-DNA) sensor were developed for a designated DNA detection. The signaling mechanism of the signaling probe (SP) shifting different from the classical conformation/flexibility change mode endows the sensor with high sensitivity. Through using thiolated or no thiolated capturing probe (CP), two 3-probe sensing structures, sensor-1 and sensor-2, were designed and constructed. The systematical comparing research results show that both sensors exhibit some similarities or big differences in sensing performance. On the one hand, the similarity in structures determines the similarity in some aspects of signaling mechanism, background signal, signal changing form, anti-fouling ability and versatility; on the other hand, the slight difference in structures also results in two opposite hybridization modes of gradual increasing resistance and gradual decreasing resistance which can affect the hybridization efficiency between the assistant probe (AP) and the SP, further producing some big differences in sensing performance, for example, apparently different signal enhancement (SE) change, point mutation discrimination ability and response speed. Under the optimized fabrication and detection conditions, both sensors feature high sensitivity for target DNAs with the detection limits of ∼10 fM for sensor-1 and ∼7 fM for sensor-2, respectively. Among many acquired sensing virtues, the sensor-1 shows a peculiar specificity adjustability which is also a highlight in this work. Copyright © 2017 Elsevier B.V. All rights reserved.

Fully Integrated, Miniature, High-Frequency Flow Probe Utilizing MEMS Leadless SOI Technology

NASA Technical Reports Server (NTRS)

Ned, Alex; Kurtz, Anthony; Shang, Tonghuo; Goodman, Scott; Giemette. Gera (d)

2013-01-01

This work focused on developing, fabricating, and fully calibrating a flowangle probe for aeronautics research by utilizing the latest microelectromechanical systems (MEMS), leadless silicon on insulator (SOI) sensor technology. While the concept of angle probes is not new, traditional devices had been relatively large due to fabrication constraints; often too large to resolve flow structures necessary for modern aeropropulsion measurements such as inlet flow distortions and vortices, secondary flows, etc. Mea surements of this kind demanded a new approach to probe design to achieve sizes on the order of 0.1 in. (.3 mm) diameter or smaller, and capable of meeting demanding requirements for accuracy and ruggedness. This approach invoked the use of stateof- the-art processing techniques to install SOI sensor chips directly onto the probe body, thus eliminating redundancy in sensor packaging and probe installation that have historically forced larger probe size. This also facilitated a better thermal match between the chip and its mount, improving stability and accuracy. Further, the leadless sensor technology with which the SOI sensing element is fabricated allows direct mounting and electrical interconnecting of the sensor to the probe body. This leadless technology allowed a rugged wire-out approach that is performed at the sensor length scale, thus achieving substantial sensor size reductions. The technology is inherently capable of high-frequency and high-accuracy performance in high temperatures and harsh environments.

Rationally designed fluorescently labeled sulfate-binding protein mutants: evaluation in the development of a sensing system for sulfate

NASA Technical Reports Server (NTRS)

Shrestha, Suresh; Salins, Lyndon L E.; Mark Ensor, C.; Daunert, Sylvia

2002-01-01

Periplasmic binding proteins from E. coli undergo large conformational changes upon binding their respective ligands. By attaching a fluorescent probe at rationally selected unique sites on the protein, these conformational changes in the protein can be monitored by measuring the changes in fluorescence intensity of the probe which allow the development of reagentless sensing systems for their corresponding ligands. In this work, we evaluated several sites on bacterial periplasmic sulfate-binding protein (SBP) for attachment of a fluorescent probe and rationally designed a reagentless sensing system for sulfate. Eight different mutants of SBP were prepared by employing the polymerase chain reaction (PCR) to introduce a unique cysteine residue at a specific location on the protein. The sites Gly55, Ser90, Ser129, Ala140, Leu145, Ser171, Val181, and Gly186 were chosen for mutagenesis by studying the three-dimensional X-ray crystal structure of SBP. An environment-sensitive fluorescent probe (MDCC) was then attached site-specifically to the protein through the sulfhydryl group of the unique cysteine residue introduced. Each fluorescent probe-conjugated SBP mutant was characterized in terms of its fluorescence properties and Ser171 was determined to be the best site for the attachment of the fluorescent probe that would allow for the development of a reagentless sensing system for sulfate. Three different environment-sensitive fluorescent probes (1,5-IAEDANS, MDCC, and acylodan) were studied with the SBP171 mutant protein. A calibration curve for sulfate was constructed using the labeled protein and relating the change in the fluorescence intensity with the amount of sulfate present in the sample. The detection limit for sulfate was found to be in the submicromolar range using this system. The selectivity of the sensing system was demonstrated by evaluating its response to other anions. A fast and selective sensing system with detection limits for sulfate in the submicromolar range was developed. Copyright 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 517-526, 2002.

Fluorescent biosensor for the detection of hyaluronidase: intensity-based ratiometric sensing and fluorescence lifetime-based sensing using a long lifetime azadioxatriangulenium (ADOTA) fluorophore.

PubMed

Chib, Rahul; Mummert, Mark; Bora, Ilkay; Laursen, Bo W; Shah, Sunil; Pendry, Robert; Gryczynski, Ignacy; Borejdo, Julian; Gryczynski, Zygmunt; Fudala, Rafal

2016-05-01

In this report, we have designed a rapid and sensitive, intensity-based ratiometric sensing as well as lifetime-based sensing probe for the detection of hyaluronidase activity. Hyaluronidase expression is known to be upregulated in various pathological conditions. We have developed a fluorescent probe by heavy labeling of hyaluronic acid with a new orange/red-emitting organic azadioxatriangulenium (ADOTA) fluorophore, which exhibits a long fluorescence lifetime (∼20 ns). The ADOTA fluorophore in water has a peak fluorescence lifetime of ∼20 ns and emission spectra centered at 560 nm. The heavily ADOTA-labeled hyaluronic acid (HA-ADOTA) shows a red shift in the peak emission wavelength (605 nm), a weak fluorescence signal, and a shorter fluorescence lifetime (∼4 ns) due to efficient self-quenching and formation of aggregates. In the presence of hyaluronidase, the brightness and fluorescence lifetime of the sample increase with a blue shift in the peak emission to its original wavelength at 560 nm. The ratio of the fluorescence intensity of the HA-ADOTA probe at 560 and 605 nm can be used as the sensing method for the detection of hyaluronidase. The cleavage of the hyaluronic acid macromolecule reduces the energy migration between ADOTA molecules, as well as the degree of self-quenching and aggregation. This probe can be efficiently used for both intensity-based ratiometric sensing as well as fluorescence lifetime-based sensing of hyaluronidase. The proposed method makes it a rapid and sensitive assay, useful for analyzing levels of hyaluronidase in relevant clinical samples like urine or plasma. Graphical Abstract Scheme showing cleavage of HA-ADOTA probe by hyaluronidase and the change in the emission spectrum of HA-ADOTA probe before and after cleavage by hyaluronidase.

Quantum Discord Determines the Interferometric Power of Quantum States

NASA Astrophysics Data System (ADS)

Girolami, Davide; Souza, Alexandre M.; Giovannetti, Vittorio; Tufarelli, Tommaso; Filgueiras, Jefferson G.; Sarthour, Roberto S.; Soares-Pinto, Diogo O.; Oliveira, Ivan S.; Adesso, Gerardo

2014-05-01

Quantum metrology exploits quantum mechanical laws to improve the precision in estimating technologically relevant parameters such as phase, frequency, or magnetic fields. Probe states are usually tailored to the particular dynamics whose parameters are being estimated. Here we consider a novel framework where quantum estimation is performed in an interferometric configuration, using bipartite probe states prepared when only the spectrum of the generating Hamiltonian is known. We introduce a figure of merit for the scheme, given by the worst-case precision over all suitable Hamiltonians, and prove that it amounts exactly to a computable measure of discord-type quantum correlations for the input probe. We complement our theoretical results with a metrology experiment, realized in a highly controllable room-temperature nuclear magnetic resonance setup, which provides a proof-of-concept demonstration for the usefulness of discord in sensing applications. Discordant probes are shown to guarantee a nonzero phase sensitivity for all the chosen generating Hamiltonians, while classically correlated probes are unable to accomplish the estimation in a worst-case setting. This work establishes a rigorous and direct operational interpretation for general quantum correlations, shedding light on their potential for quantum technology.

Turbofan aft duct suppressor study. Contractor's data report of mode probe signal data

NASA Technical Reports Server (NTRS)

Fiske, G. H.; Motsinger, R. E.; Syed, A. A.; Joshi, M. C.; Kraft, R. E.

1983-01-01

Acoustic modal distributions were measured in a fan test model having an annular exhaust duct for comparison with theoretically predicted acoustic suppression values. This report contains the amplitude and phase data of the acoustic signals sensed by the transducers of the two mode probes employed in the measurement. Each mode probe consisted of an array of 12 transducers sensing the acoustic field at three axial positions and four radial positions.

Integrated miniature fluorescent probe to leverage the sensing potential of ZnO quantum dots for the detection of copper (II) ions.

PubMed

Ng, Sing Muk; Wong, Derrick Sing Nguong; Phung, Jane Hui Chiun; Chin, Suk Fun; Chua, Hong Siang

2013-11-15

Quantum dots are fluorescent semiconductor nanoparticles that can be utilised for sensing applications. This paper evaluates the ability to leverage their analytical potential using an integrated fluorescent sensing probe that is portable, cost effective and simple to handle. ZnO quantum dots were prepared using the simple sol-gel hydrolysis method at ambient conditions and found to be significantly and specifically quenched by copper (II) ions. This ZnO quantum dots system has been incorporated into an in-house developed miniature fluorescent probe for the detection of copper (II) ions in aqueous medium. The probe was developed using a low power handheld black light as excitation source and three photo-detectors as sensor. The sensing chamber placed between the light source and detectors was made of 4-sided clear quartz windows. The chamber was housed within a dark compartment to avoid stray light interference. The probe was operated using a microcontroller (Arduino Uno Revision 3) that has been programmed with the analytical response and the working algorithm of the electronics. The probe was sourced with a 12 V rechargeable battery pack and the analytical readouts were given directly using a LCD display panel. Analytical optimisations of the ZnO quantum dots system and the probe have been performed and further described. The probe was found to have a linear response range up to 0.45 mM (R(2)=0.9930) towards copper (II) ion with a limit of detection of 7.68×10(-7) M. The probe has high repeatable and reliable performance. Copyright © 2013 Elsevier B.V. All rights reserved.

A multi-signal fluorescent probe for simultaneously distinguishing and sequentially sensing cysteine/homocysteine, glutathione, and hydrogen sulfide in living cells† †Electronic supplementary information (ESI) available: Experimental details for chemical synthesis of all compounds, chemical structure characterization, supplementary spectra of probe, and fluorescence imaging methods and data. See DOI: 10.1039/c7sc00423k Click here for additional data file.

PubMed Central

He, Longwei; Yang, Xueling; Xu, Kaixin; Kong, Xiuqi

2017-01-01

Biothiols, which have a close network of generation and metabolic pathways among them, are essential reactive sulfur species (RSS) in the cells and play vital roles in human physiology. However, biothiols possess highly similar chemical structures and properties, resulting in it being an enormous challenge to simultaneously discriminate them from each other. Herein, we develop a unique fluorescent probe (HMN) for not only simultaneously distinguishing Cys/Hcy, GSH, and H2S from each other, but also sequentially sensing Cys/Hcy/GSH and H2S using a multi-channel fluorescence mode for the first time. When responding to the respective biothiols, the robust probe exhibits multiple sets of fluorescence signals at three distinct emission bands (blue-green-red). The new probe can also sense H2S at different concentration levels with changes of fluorescence at the blue and red emission bands. In addition, the novel probe HMN is able to discriminate and sequentially sense biothiols in biological environments via three-color fluorescence imaging. We expect that the development of the robust probe HMN will provide a powerful strategy to design fluorescent probes for the discrimination and sequential detection of biothiols, and offer a promising tool for exploring the interrelated roles of biothiols in various physiological and pathological conditions. PMID:28989659

Design and Investigation of Optical Properties of N-(Rhodamine-B)-Lactam-Ethylenediamine (RhB-EDA) Fluorescent Probe.

PubMed

Soršak, Eva; Volmajer Valh, Julija; Korent Urek, Špela; Lobnik, Aleksandra

2018-04-14

This study presents chemical modification of a Rhodamine B (RhB) sensor probe by ethylenediamine (EDA), and investigation of its spectral as well as sensor properties to the various metals. The synthesised N -(Rhodamine-B)-lactam-ethylenediamine (RhB-EDA) fluorescent probe shows interesting optical sensor properties, and high sensitivity and selectivity to Ag⁺ ions among all the tested metal ions (K⁺, Mg 2+ , Cu 2+ , Ni 2+ , Fe 2+ , Pb 2+ , Na⁺, Mn 2+ , Li⁺, Al 3+ , Co 2+ , Hg 2+ , Sr 2+ , Ca 2+ , Ag⁺, Cd 2+ and Zn 2+ ), while the well-known Rhodamine B (RhB) fluorescent probe shows much less sensitivity to Ag⁺ ions, but high sensitivity to Fe 2+ ions. The novel fluorescent sensor probe RhB-EDA has the capabilities to sense Ag⁺ ions up to µM ranges by using the fluorescence quenching approach. The probe displayed a dynamic response to Ag⁺ in the range of 0.43 × 10 -3 -10 -6 M with a detection limit of 0.1 μM. The sensing system of an RhB-EDA novel fluorescent probe was optimised according to the spectral properties, effect of pH and buffer, photostability, incubation time, sensitivity, and selectivity. Since all the spectral and sensing properties were tested in green aqueous media, although many other similar sensor systems rely on organic solvent solutions, the RhB-EDA sensing probe may be a good candidate for measuring Ag⁺ ions in real-life applications.

Langmuir Probe Diagnostics of Pulsed Plasma Doping System

NASA Astrophysics Data System (ADS)

Lei, Yu; Overzet, Lawrence J.; Felch, Susan B.; Fang, Ziwei; Koo, Bon-Woong; Goeckner, Matthew J.

2002-10-01

Pulsed plasma doping (P2LAD) is a potential solution to implement ultra-shallow junctions. In this study, Langmuir probe diagnostics techniques were investigated thoroughly for its application to P2LAD system, and the current sensing scheme using batteries and a 'downstairs' load resistor turned out to be the most reliable. Severe limitations of current transformers were found in diagnostics of pulsed plasma. A floating probe was proven to be good at monitoring the disturbances of the Langmuir probe and the cathode voltage. With the above technique, time-resolved Langmuir probe measurements have been carried out in a P2LAD system. The Langmuir probe data in Ar plasma indicate that during a 20 microns long implant pulse the plasma density ranges from 1E9 1E10 cm-3 and the electron temperature ranges from 0.4 to 14 eV. Between the pulses, the density keeps at the high level for 30 ms and then decays exponentially until reaching the range of 3E8 1E9 cm-3, which demonstrates the presence of residual plasma between pulses. A non-zero plasma density during the afterglow is also observed for BF3 plasma. Significant amounts of primary electron and electron beams are present during the ignition and ensuing steady region in both Ar and BF3 plasmas while they are much stronger in BF3 plasma. Plasma density is observed to increase with cathode voltage and pressure while the electron temperature is mainly influenced by the pressure. An overshoot of the cathode voltage during the afterglow region was found, and it significantly influences the plasma potential during the afterglow.

Mapping Sensory Spots for Moderate Temperatures on the Back of Hand.

PubMed

Yang, Fan; Chen, Guixu; Zhou, Sikai; Han, Danhong; Xu, Jingjing; Xu, Shengyong

2017-12-04

Thermosensation with thermoreceptors plays an important role in maintaining body temperature at an optimal state and avoiding potential damage caused by harmful hot or cold environmental temperatures. In this work, the locations of sensory spots for sensing moderate temperatures of 40-50 °C on the back of the hands of young Chinese people were mapped in a blind-test manner with a thermal probe of 1.0 mm spatial resolution. The number of sensory spots increased along with the testing temperature; however, the surface density of sensory spots was remarkably lower than those reported previously. The locations of the spots were irregularly distributed and subject-dependent. Even for the same subject, the number and location of sensory spots were unbalanced and asymmetric between the left and right hands. The results may offer valuable information for designing artificial electronic skin and wearable devices, as well as for clinical applications.

Radiometric Thermometry for Wearable Deep Tissue Monitoring

NASA Astrophysics Data System (ADS)

Momenroodaki, Parisa

Microwave thermometry is an attractive non-invasive method for measuring internal body temperature. This approach has the potential of enabling a wearable device that can continuously monitor core body temperature. There are a number of health-related applications in both diagnostics and therapy, which can benefit from the knowledge of core body temperature. However,there are a limited number of device solutions, which are usually not wearable or cannot continuously monitor internal body temperature non-invasively. In this thesis, a possible path toward implementing such a thermometer is presented. The device operates in the "quiet" frequency band of 1.4 GHz which is chosen as a compromise between sensing depth and radio frequency interference (RFI). A major challenge in microwave thermometry is detecting small temperature variations of deep tissue layers from surface (skin) measurements. The type and thickness of tissue materials significantly affect the design of the probe, which has the function of receiving black-body radiation from tissues beneath it and coupling the power to a sensitive radiometric receiver. High dielectric constant contrast between skin, fat (/bone), and muscle layers suggests structures with dominant tangential component of the electric field, such as a patch or slot. Adding a layer of low-loss,low-dielectric constant superstrate can further reduce the contribution of superficial tissue layers in the received thermal noise. Several probe types are designed using a full-wave electromagnetic simulator, with a goal of maximizing the power reception from deep tissue layers. The designs are validated with a second software tool and various measurements. A stable, narrow-band, and highly sensitive radiometer is developed, enabling the device to operate in a non-shielded RF environment.To use the microwave thermometer in a RF congested environment, not only narrow-band probe and radiometers are used but an additional probe is introduced for observing the environmental interference. By applying an adaptive filter, the effect of RFI is mitigated in long-term measurements. Several solid and liquid tissue phantoms, required for accurate modeling of the probe and human body interaction, are also developed. The concept of human body microwave thermometry is validated through several measurements on the single-layer and multiple-layer tissue phantoms as well as on the surface of the human body, specifically on the cheek where the internal temperature can easily be changed and independently measured with a thermocouple. Measurement results prove the capability of the device in tracking the temperature of buried tissue layer phantoms to within 0.2K, as well as monitoring internal human body temperature.

Capacitively coupled RF voltage probe having optimized flux linkage

DOEpatents

Moore, James A.; Sparks, Dennis O.

1999-02-02

An RF sensor having a novel current sensing probe and a voltage sensing probe to measure voltage and current. The current sensor is disposed in a transmission line to link all of the flux generated by the flowing current in order to obtain an accurate measurement. The voltage sensor is a flat plate which operates as a capacitive plate to sense voltage on a center conductor of the transmission line, in which the measured voltage is obtained across a resistance leg of a R-C differentiator circuit formed by the characteristic impedance of a connecting transmission line and a capacitance of the plate, which is positioned proximal to the center conductor.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nabeel A. Riza

The goals of the second six months of the Phase 2 of this project were to conduct first time experimental studies using optical designs and some initial hardware developed in the first 6 months of Phase 2. One focus is to modify the SiC chip optical properties to enable gas species sensing with a specific gas species under high temperature and pressure. The goal was to acquire sensing test data using two example inert and safe gases and show gas discrimination abilities. A high pressure gas mixing chamber was to be designed and assembled to achieve the mentioned gas sensingmore » needs. Another goal was to initiate high temperature probe design by developing and testing a probe design that leads to accurately measuring the thickness of the deployed SiC sensor chip to enable accurate overall sensor system design. The third goal of this phase of the project was to test the SiC chip under high pressure conditions using the earlier designed calibration cell to enable it to act as a pressure sensor when doing gas detection. In this case, experiments using a controlled pressure system were to deliver repeatable pressure measurement data. All these goals have been achieved and are described in detail in the report. Both design process and diagrams for the mechanical elements as well as the optical systems are provided. Photographs or schematics of the fabricated hardware are provided. Experimental data from the three optical sensor systems (i.e., Thickness, pressure, and gas species) is provided. The design and experimentation results are summarized to give positive conclusions on the proposed novel high temperature high pressure gas species detection optical sensor technology.« less

Circumferential pressure probe

NASA Technical Reports Server (NTRS)

Holmes, Harlan K. (Inventor); Moore, Thomas C. (Inventor); Fantl, Andrew J. (Inventor)

1989-01-01

A probe for measuring circumferential pressure inside a body cavity is disclosed. In the preferred embodiment, a urodynamic pressure measurement probe for evaluating human urinary sphincter function is disclosed. Along the length of the probe are disposed a multiplicity of deformable wall sensors which typically comprise support tube sections with flexible side wall areas. These are arranged along the length of the probe in two areas, one just proximal to the tip for the sensing of fluid pressure inside the bladder, and five in the sensing section which is positioned within the urethra at the point at which the urinary sphincter constricts to control the flow of urine. The remainder of the length of the probe comprises multiple rigid support tube sections interspersed with flexible support tube sections in the form of bellows to provide flexibility.

Integrated sensing and actuation of dielectric elastomer actuator

NASA Astrophysics Data System (ADS)

Ye, Zhihang; Chen, Zheng

2017-04-01

Dielectric elastomer (DE) is a type of soft actuating material, the shape of which can be changed under electrical voltage stimuli. DE materials have great potential in applications involving energy harvesters, micro-manipulators, and adaptive optics. In this paper, a stripe DE actuator with integrated sensing and actuation is designed and fabricated, and characterized through several experiments. Considering the actuator's capacitor-like structure and its deform mechanism, detecting the actuator's displacement through the actuator's circuit feature is a potential approach. A self-sensing scheme that adds a high frequency probing signal into actuation signal is developed. A fast Fourier transform (FFT) algorithm is used to extract the magnitude change of the probing signal, and a non-linear fitting method and artificial neural network (ANN) approach are utilized to reflect the relationship between the probing signal and the actuator's displacement. Experimental results showed this structure has capability of performing self-sensing and actuation, simultaneously. With an enhanced ANN, the self-sensing scheme can achieve 2.5% accuracy.

Structure and dynamics of biomembranes in room-temperature ionic liquid water solutions studied by neutron scattering and by molecular dynamics simulations

NASA Astrophysics Data System (ADS)

Benedetto, Antonio; Ballone, Pietro

2018-05-01

Increasing attention is being devoted to the interaction of a new class of organic ionic liquids known as room-temperature ionic liquids (RTILs) with biomolecules, partly because of health and environment concerns, and, even more, for the prospect of exciting new applications in biomedicine, sensing and energy technologies. Here we focus on the interaction between RTILs and phospholipid bilayers that are well-accepted models for bio-membranes. We discuss how neutron scattering has been used to probe both the structure and the dynamics of these systems, and how its integration with molecular dynamics simulation has allowed the determination of the microscopic details of their interaction.

Mass Spectrometry for Planetary Probes: Past, Present and Future

NASA Technical Reports Server (NTRS)

Niemann, Hasso B.; Harpold, Dan N.; Jamieson, Brian G.; Mahaffy, Paul R.

2005-01-01

Atmospheric entry probes present a unique opportunity for performing quantitative analysis of extra-terrestrial atmospheres in cases where remote sensing alone may not be sufficient and measurements with balloons or aircraft is not practical. An entry probe can provide a complete vertical profile of atmospheric parameters including chemical composition, which cannot be obtained with most other techniques. There are, however, unique challenges associated with building instruments for an entry probe, as compared to orbiters, landers, or rovers. Conditions during atmospheric entry are extreme, there are inherent time constraints due to the short duration of the experiment, and the instrument experiences rapid environmental changes in temperature and pressure as it descends. In addition, there are resource limitations, i.e. mass, power, size and bandwidth. Finally, the demands on the instrument design are determined in large part by conditions (pressure, temperature, composition) unique to the particular body under study, and as a result there is no one-size-fits-all instrument for an atmospheric probe. Many of these requirements can be more easily met by miniaturizing the probe instrument. Our experience building mass spectrometers for atmospheric entry probes leads us to believe that the time is right for a fundamental change in the way spaceflight mass spectrometers are built. The emergence over the past twenty years of Micro-electro- mechanical Systems (MEMS), utilizing lithographic semiconductor fabrication techniques to produce instrument systems in miniature, holds great promise for application to spaceflight mass spectrometry. A highly miniaturized, high performance and low-power mass spectrometer would be an enormous benefit to future entry probe missions, allowing, for example, parallel measurements (e.g., multiple simultaneous gas chromatographic analyses and direct atmospheric leaks.) Such an instrument would also enable mass spectrometry on board small multiple entry probes. In the development of a MEMS Mass Spectrometer, the challenge facing us is to move beyond the proof-of-concept, where research dollars tend to focus, and carry out the detailed work of developing a high performance mass spectrometer system on a chip which meets the unique technical requirements for an atmospheric entry probe described above.

Methodology Development for the Reconstruction of the ESA Huygens Probe Entry and Descent Trajectory

NASA Astrophysics Data System (ADS)

Kazeminejad, B.

2005-01-01

The European Space Agency's (ESA) Huygens probe performed a successful entry and descent into Titan's atmosphere on January 14, 2005, and landed safely on the satellite's surface. A methodology was developed, implemented, and tested to reconstruct the Huygens probe trajectory from its various science and engineering measurements, which were performed during the probe's entry and descent to the surface of Titan, Saturn's largest moon. The probe trajectory reconstruction is an essential effort that has to be done as early as possible in the post-flight data analysis phase as it guarantees a correct and consistent interpretation of all the experiment data and furthermore provides a reference set of data for "ground-truthing" orbiter remote sensing measurements. The entry trajectory is reconstructed from the measured probe aerodynamic drag force, which also provides a means to derive the upper atmospheric properties like density, pressure, and temperature. The descent phase reconstruction is based upon a combination of various atmospheric measurements such as pressure, temperature, composition, speed of sound, and wind speed. A significant amount of effort was spent to outline and implement a least-squares trajectory estimation algorithm that provides a means to match the entry and descent trajectory portions in case of discontinuity. An extensive test campaign of the algorithm is presented which used the Huygens Synthetic Dataset (HSDS) developed by the Huygens Project Scientist Team at ESA/ESTEC as a test bed. This dataset comprises the simulated sensor output (and the corresponding measurement noise and uncertainty) of all the relevant probe instruments. The test campaign clearly showed that the proposed methodology is capable of utilizing all the relevant probe data, and will provide the best estimate of the probe trajectory once real instrument measurements from the actual probe mission are available. As a further test case using actual flight data the NASA Mars Pathfinder entry and descent trajectory and the space craft attitude was reconstructed from the 3-axis accelerometer measurements which are archived on the Planetary Data System. The results are consistent with previously published reconstruction efforts.

Use of a pitot probe for determining wing section drag in flight

NASA Technical Reports Server (NTRS)

Saltzman, E. J.

1975-01-01

A wake traversing probe was used to obtain section drag and wake profile data from the wing of a sailplane. The transducer sensed total pressure defect in the wake as well as freestream total pressure on both sides of the sensing element when the probe moved beyond the wake. Profiles of wake total pressure defects plotted as a function of distance above and below the trailing edge plane were averaged for calculating section drag coefficients for flights at low dynamic pressures.

Design and Evaluation of Dedicated Smartphone Applications for Collaborative Science Education

NASA Astrophysics Data System (ADS)

Fertitta, John A., Jr.

2011-12-01

Over the past several years, the use of scientific probes is becoming more common in science classrooms. The goal of teaching with these science probes is to engage students in inquiry-based learning. However, they are often complicated and stationary, forcing experiments to remain in the classroom and limiting their use. The Internet System for Networked Sensor Experimentation (iSENSE) was created to address these limitations. iSENSE is a web-system for storing and visualizing sensor data. The project also includes a hardware package, the PINPoint, that interfaces to existing probes, and acts as a probe itself. As the mobile phone industry continues to advance, we are beginning to see smartphones that are just as powerful, if not more powerful, than many desktop computers. These devices are often equipped with advanced sensors, making them as capable as some science probes at a lower cost. With this background, this thesis explores the use of smartphones in secondary school science classrooms. By collaborating with one teacher, three custom applications were developed for four separate curriculum-based learning activities. The smartphones replaced existing traditional tools and science probes. Some data collected with the smartphones were uploaded to the iSENSE web-system for analysis. Student use of the smartphones and the subsequent scientific visualizations using the iSENSE web-system were observed. A teacher interview was conducted afterward. It was found that a collaborative design process involving the teacher resulted in the successful integration of smartphone applications into learning activities. In one case, the smartphones and use of iSENSE did not improve the students' understanding of the learning objectives. In several others, however, the smartphones out-performed traditional probeware as a data collector, and with the classroom teachers guidance, the iSENSE web-system facilitated more in-depth discussions of the data.

A fluorogenic substrate of beta-lactamases and its potential as a probe to detect the bacteria resistant to the third-generation oxyimino-cephalosporins.

PubMed

Thai, Hien Bao Dieu; Yu, Jin Kyung; Park, Byung Sun; Park, Yeon-Joon; Min, Sun-Joon; Ahn, Dae-Ro

2016-03-15

We devised and synthesized a fluorogenic substrate of β-lactamases as a probe to detect the activity of the enzymes. Fluorescence of the probe emitted upon treatment of a β-lactamase and increased proportionally to the concentration of the enzyme, demonstrating its sensing property for the activity of the enzyme. We also showed that the probe could be utilized to assay the enzyme and to determine kinetic parameters of the enzyme. Moreover, the probe was able to detect resistance to the third-generation oxyimino-cephalosporin-derived antibiotics such as cefotaxime and ceftazidime. In particular, the probe could identify the ceftazidime-resistance in bacteria that was not detectable using conventional pH-sensing materials, indicating the practical utility of the probe. Copyright © 2015 Elsevier B.V. All rights reserved.

Low-control and robust quantum refrigerator and applications with electronic spins in diamond

NASA Astrophysics Data System (ADS)

Mohammady, M. Hamed; Choi, Hyeongrak; Trusheim, Matthew E.; Bayat, Abolfazl; Englund, Dirk; Omar, Yasser

2018-04-01

We propose a general protocol for low-control refrigeration and thermometry of thermal qubits, which can be implemented using electronic spins in diamond. The refrigeration is implemented by a probe, consisting of a network of interacting spins. The protocol involves two operations: (i) free evolution of the probe; and (ii) a swap gate between one spin in the probe and the thermal qubit we wish to cool. We show that if the initial state of the probe falls within a suitable range, and the free evolution of the probe is both unital and conserves the excitation in the z direction, then the cooling protocol will always succeed, with an efficiency that depends on the rate of spin dephasing and the swap-gate fidelity. Furthermore, measuring the probe after it has cooled many qubits provides an estimate of their temperature. We provide a specific example where the probe is a Heisenberg spin chain, and suggest a physical implementation using electronic spins in diamond. Here, the probe is constituted of nitrogen vacancy (NV) centers, while the thermal qubits are dark spins. By using a novel pulse sequence, a chain of NV centers can be made to evolve according to a Heisenberg Hamiltonian. This proposal allows for a range of applications, such as NV-based nuclear magnetic resonance of photosensitive molecules kept in a dark spot on a sample, and it opens up possibilities for the study of quantum thermodynamics, environment-assisted sensing, and many-body physics.

Global navigation satellite sounding of the atmosphere and GNSS altimetry : prospects for geosciences

NASA Technical Reports Server (NTRS)

Yunck, Tom P.; Hajj, George A.

2003-01-01

The vast illuminating power of the Global Positioning System (GPS), which transformed space geodesy in the 199Os, is now serving to probe the earth's fluid envelope in unique ways. Three distinct techniques have emerged: ground-based sensing of the integrated atmospheric moisture; space-based profiling of atmospheric refractivity, pressure, temperature, moisture, and other properties by active limb sounding; and surface (ocean and ice) altimetry and scatterometry with reflected signals detected from space. Ground-based GPS moisture sensing is already in provisional use for numerical weather prediction. Limb sounding, while less mature, offers a bevy of attractions, including high accuracy, stability, and vertical resolution; all-weather operation; and exceptionally low cost. GPS bistatic radar, r 'reflectometry,' is the least advanced but shows promise for a number of niche applications.

Laboratory demonstration and field verification of a Wireless Cookstove Sensing System (WiCS) for determining cooking duration and fuel consumption

DOE PAGES

Graham, Eric A.; Patange, Omkar; Lukac, Martin; ...

2014-08-27

With improved cookstoves (ICs) increasingly distributed to households for a range of air pollution interventions and carbon-credit programs, it has become necessary to accurately monitor the duration of cooking and the amount of fuel consumed. In this study, laboratory trials were used to create temperature-based algorithms for quantifying cooking duration and estimating fuel consumption from stove temperatures. Field validation of the algorithms employed a Wireless Cookstove Sensing System (WiCS) that offers remote, low-cost temperature sensing and the wireless transmission of temperature data to a centralized database using local cellular networks. Field trials included 68 unscripted household cooking events. In themore » laboratory, temperature responses of the IC body and that of a removable temperature probe (J-bar) followed well-known physical models during cooking, indicating that location of the temperature sensor is not critical. In the laboratory, the classification correctly identified active cooking 97.2% of the time. In the field, the cooking duration was not statistically different from that recorded by trained volunteers; the average difference between calculated and observed cooking times was 0.03 ± 0.31 h (mean ± SD). In the laboratory, energy flux from the IC was calculated using temperatures measured by the J-bar and on the IC body and found to be proportional to the total energy in the consumed fuel, with an r 2 correlation value of 0.95. Here in the field, the average fuel consumption was calculated to be 0.97 ± 0.32 kg compared to that recorded by volunteers of 1.19 ± 0.37 kg with an average difference between calculated and observed fuel mass of 0.21 ± 0.37 kg per event. Finally, despite wide variation in observed cooking duration and fuel consumption per event, a relatively constant rate of fuel consumption of 0.48 kg h -1 was calculated for users of the same type of IC.« less

Molecular Probes: An Innovative Technology for Monitoring Membrane Processes

NASA Astrophysics Data System (ADS)

Santoro, Sergio

The ultimate objective of this study is to use molecular probes as an innovative and alternative technology contributing to the advance of membrane science by monitoring membrane processes in-situ, on-line and at sub-micron scale. An optical sensor for oxygen sensing was developed by the immobilization of tris (1,10-phenanthroline) ruthenium (II) (Ru(phen)3) in a dense polymeric membrane made of polystyrene (PS) or Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). The emission of the probe was quenched by both the temperature and by the oxygen. Moreover, the oxygen sensitivity was affected by the oxygen permeability of the membrane. The evaluation of the oxygen concentration is prone to errors since the emission of a single probe depends on several parameters (i.e. optical path, source intensity). The correction of these artefacts was obtained by the immobilization of a second luminescent molecule non-sensitive to the oxygen, Coumarin. The potential of the luminescent ratiometric sensor for the non-invasive monitoring of oxygen in food packaging using polymeric films with different oxygen permeability was evaluated. Emphasis was given to the efficiency of the optical sensor for the on-line, in-situ and non invasive monitoring of the oxygen by comparing the experimental data with a model which takes into account the oxygen permeability of the packaging materials evaluated independently. A nano-thermometer based on silica nano-particles doped with Ru(phen)3 was developed. A systematic study shows how it is possible to control the properties of the nano-particles as well as their temperature sensitivity. The nano-thermometer was immobilized on a membrane surface by dip-coating providing information about the temperature on the membrane surface. Hydrophobic porous membrane made of Poly(vinylidene fluoride) was prepared via electrospinning and employed in a direct contact membrane distillation process. Using a designed membrane module and a membrane doped with Ru(phen)3 the on-line mapping of the temperature on the membrane's surface was evaluated. None None None None

Research on the Sensing Performance of the Tuning Fork-Probe as a Micro Interaction Sensor

PubMed Central

Gao, Fengli; Li, Xide

2015-01-01

The shear force position system has been widely used in scanning near-field optical microscopy (SNOM) and recently extended into the force sensing area. The dynamic properties of a tuning fork (TF), the core component of this system, directly determine the sensing performance of the shear positioning system. Here, we combine experimental results and finite element method (FEM) analysis to investigate the dynamic behavior of the TF probe assembled structure (TF-probe). Results from experiments under varying atmospheric pressures illustrate that the oscillation amplitude of the TF-probe is linearly related to the quality factor, suggesting that decreasing the pressure will dramatically increase the quality factor. The results from FEM analysis reveal the influences of various parameters on the resonant performance of the TF-probe. We compared numerical results of the frequency spectrum with the experimental data collected by our recently developed laser Doppler vibrometer system. Then, we investigated the parameters affecting spatial resolution of the SNOM and the dynamic response of the TF-probe under longitudinal and transverse interactions. It is found that the interactions in transverse direction is much more sensitive than that in the longitudinal direction. Finally, the TF-probe was used to measure the friction coefficient of a silica–silica interface. PMID:26404310

Intracellular O2 sensing probe based on cell-penetrating phosphorescent nanoparticles.

PubMed

Fercher, Andreas; Borisov, Sergey M; Zhdanov, Alexander V; Klimant, Ingo; Papkovsky, Dmitri B

2011-07-26

A new intracellular O(2) (icO(2)) sensing probe is presented, which comprises a nanoparticle (NP) formulation of a cationic polymer Eudragit RL-100 and a hydrophobic phosphorescent dye Pt(II)-tetrakis(pentafluorophenyl)porphyrin (PtPFPP). Using the time-resolved fluorescence (TR-F) plate reader set-up, cell loading was investigated in detail, particularly the effects of probe concentration, loading time, serum content in the medium, cell type, density, etc. The use of a fluorescent analogue of the probe in conjunction with confocal microscopy and flow cytometry analysis, revealed that cellular uptake of the NPs is driven by nonspecific energy-dependent endocytosis and that the probe localizes inside the cell close to the nucleus. Probe calibration in biological environment was performed, which allowed conversion of measured phosphorescence lifetime signals into icO(2) concentration (μM). Its analytical performance in icO(2) sensing experiments was demonstrated by monitoring metabolic responses of mouse embryonic fibroblast cells under ambient and hypoxic macroenvironment. The NP probe was seen to generate stable and reproducible signals in different types of mammalian cells and robust responses to their metabolic stimulation, thus allowing accurate quantitative analysis. High brightness and photostability allow its use in screening experiments with cell populations on a commercial TR-F reader, and for single cell analysis on a fluorescent microscope.

AIE active multianalyte fluorescent probe for the detection of Cu2+, Ni2+ and Hg2+ ions.

PubMed

Pannipara, Mehboobali; Al-Sehemi, Abdullah G; Irfan, Ahmad; Assiri, Mohammed; Kalam, Abul; Al-Ammari, Yahya S

2018-08-05

A novel pyrazolyl chromene derivative (Probe 1) displaying aggregation induced emission (AIE) properties that capable of sensing of multiple metal ions has been designed and synthesized. The multi analyte probe exhibits selective sensing for Cu 2+ and Ni 2+ ions via fluorescence turn-off mechanism and ratiometric selectivity for Hg 2+ ions in aqueous media. The extent of binding of the probe with sensitive metal ions has been demonstrated. The experimental results were further investigated by computational means by optimizing the ground state geometries of Probe 1 and its various metal complexes for Probe 1-Ni, Probe 1-Hg and Probe 1-Cu using density functional theory (DFT) at B3LYP/6-31+g(d,p) (LANL2DZ) level. On the basis of binding energies, the stability of metal complexes has been studied. In Probe 1-Ni and Probe 1-Cu complexes, charge transfer has been observed from Probe 1 to metal ions revealing ligand to metal charge transfer (LMCT) while in Probe1-Hg complex LMCT as well as intra-molecular charge tranfer (ICT) within Probe 1. Copyright © 2018 Elsevier B.V. All rights reserved.

Microgravity combustion discipline working group summary of requirements for noncontact temperature measurements

NASA Technical Reports Server (NTRS)

Sacksteder, Kurt

1988-01-01

Current efforts of the Microgravity Combustion Working Group are summarized and the temperature measurement requirements for the combustion studies are defined. Many of the combustion systems that are studied in the low gravity environment are near-limit systems, that is, systems that are acting near the limit of flammability in terms of oxygen concentration or fuel concentration. Systems of this type are normally weak in the sense that there is a delicate balance between the heat released in the flame and the heat required to sustain the flame. Intrusive or perturbative temperature measurement probes can be inaccurate in these situations and in the limiting case extinguish the flame. Noncontact techniques then become the only way to obtain the required measurements. Noncontact measurement requirements for each of the three thermodynamic phases are described in terms of spatial and temporal resolution and temperature range.

Quantum-circuit refrigerator

NASA Astrophysics Data System (ADS)

Tan, Kuan Yen; Partanen, Matti; Lake, Russell E.; Govenius, Joonas; Masuda, Shumpei; Möttönen, Mikko

2017-05-01

Quantum technology promises revolutionizing applications in information processing, communications, sensing and modelling. However, efficient on-demand cooling of the functional quantum degrees of freedom remains challenging in many solid-state implementations, such as superconducting circuits. Here we demonstrate direct cooling of a superconducting resonator mode using voltage-controllable electron tunnelling in a nanoscale refrigerator. This result is revealed by a decreased electron temperature at a resonator-coupled probe resistor, even for an elevated electron temperature at the refrigerator. Our conclusions are verified by control experiments and by a good quantitative agreement between theory and experimental observations at various operation voltages and bath temperatures. In the future, we aim to remove spurious dissipation introduced by our refrigerator and to decrease the operational temperature. Such an ideal quantum-circuit refrigerator has potential applications in the initialization of quantum electric devices. In the superconducting quantum computer, for example, fast and accurate reset of the quantum memory is needed.

Study on the SPR responses of various DNA probe concentrations by parallel scan spectral SPR imaging

NASA Astrophysics Data System (ADS)

Ma, Suihua; Liu, Le; Lu, Weiping; Zhang, Yaou; He, Yonghong; Guo, Jihua

2008-12-01

SPR sensors have become a high sensitive and label free method for characterizing and quantifying chemical and biochemical interactions. However, the relations between the SPR refractive index response and the property (such as concentrations) of biochemical probes are still lacking. In this paper, an experimental study on the SPR responses of varies concentrations of Legionella pneumophila mip DNA probes is presented. We developed a novel two-dimensional SPR sensing technique-parallel scan spectral SPR imaging-to detect an array of mip gene probes. This technique offers quantitative refractive index information with a high sensing throughput. By detecting mip DNA probes with different concentrations, we obtained the relations between the SPR refractive index response and the concentrations of mip DNA probes. These results are valuable for design and developing SPR based mip gene biochips.

Microneedle arrays for biosensing and drug delivery

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Joseph; Windmiller, Joshua Ray; Narayan, Roger

Methods, structures, and systems are disclosed for biosensing and drug delivery techniques. In one aspect, a^ device for detecting an analyte and/or releasing a biochemical into a biological fluid can include an array of hollowed needles, in which each needle includes a protruded needle structure including an exterior wall forming a hollow interior and an opening at a terminal end of the protruded needle structure that exposes the hollow interior, and a probe inside the exterior wall to interact with one or more chemical or biological substances that come in contact with the probe via the opening to produce amore » probe sensing signal, and an array of wires that are coupled to probes of the array of hollowed needles, respectively, each wire being electrically conductive to transmit the probe sensing signal produced by a respective probe.« less

Microneedle arrays for biosensing and drug delivery

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Joseph; Windmiller, Joshua Ray; Narayan, Roger

Methods, structures, and systems are disclosed for biosensing and drug delivery techniques. In one aspect, a device for detecting an analyte and/or releasing a biochemical into a biological fluid can include an array of hollowed needles, in which each needle includes a protruded needle structure including an exterior wall forming a hollow interior and an opening at a terminal end of the protruded needle structure that exposes the hollow interior, and a probe inside the exterior wall to interact with one or more chemical or biological substances that come in contact with the probe via the opening to produce amore » probe sensing signal, and an array of wires that are coupled to probes of the array of hollowed needles, respectively, each wire being electrically conductive to transmit the probe sensing signal produced by a respective probe.« less

A Writing Template for Probing Students' Botanical Sense of Place

ERIC Educational Resources Information Center

Wandersee, James H.; Clary, Renee M.; Guzman, Sandra M.

2006-01-01

Writing can be a powerful tool for learning biology. Writing assignments in biology could help students personalize and understand the biology knowledge they are studying. In this article, the authors present the "Botanical Sense of Place" (BSP), a convenient and easy-to-use writing template that they developed to elicit and probe students' prior…

Negative measurement sensitivity values of planar capacitive imaging probes

NASA Astrophysics Data System (ADS)

Yin, Xiaokang; Chen, Guoming; Li, Wei; Hutchins, David

2014-02-01

The measurement sensitivity distribution of planar capacitive imaging (CI) probes describes how effectively each region in the sensing area is contributing to the measured charge signal on the sensing electrode. It can be used to determine the imaging ability of a CI probe. It is found in previous work that, there are regions in the sensing area where the change of the charge output and the change of targeting physical parameter are of opposite trends. This opposite correlation implies that the measurement sensitivity values in such regions are negative. In this work, the cause of negative sensitivity is discussed. Experiments are also designed and performed so as to verify the existence of negative sensitivity and study the factors that may affect the negative sensitivity distributions.

Fire and Ice: Thermoluminescent Temperature Sensing in High-Explosive Detonations and Optical Characterization Methods for Glacier Ice Boreholes

NASA Astrophysics Data System (ADS)

Mah, Merlin Lyn

The environment around a detonating high explosive is incredibly energetic and dynamic, generating shock waves, turbulent mixing, chemical reactions, and temperature excursions of thousands of Kelvin. Probing this violent but short-lived phenomena requires durable sensors with fast response times. By contrast, the glacier ice sheets of Antarctica and Greenland change on geologic time scales; the accumulation and compression of snow into ice preserves samples of atmospheric gas, dust, and volcanic ash, while the crystal orientations of the ice reflect its conditions and movement over hundreds of thousands of years. Here, difficulty of characterization stems primarily from the location, scale, and depth of the ice sheet. This work describes new sensing technologies for both of these environments. Microparticles of thermoluminescent materials are proposed as high-survivability, bulk-deployable temperature sensors for applications such as assessing bioagent inactivation. A technique to reconstruct thermal history from subsequent thermoluminescence observations is described. MEMS devices were designed and fabricated to assist in non-detonation testing: large-area electrostatic membrane actuators were used to apply mechanical stress to thermoluminescent Y2O3 :Tb thin film, and microheaters impose rapid temperature excursions upon particles of Mg2SiO4:Tb,Co to demonstrate predictable thermoluminescent response. Closed- and open-chamber explosive detonation tests using dosimetric LiF:Mg,Ti and two experimental thermometry materials were performed to test survivability and attempt thermal event reconstruction. Two borehole logging devices are described for optical characterization of glacier ice. For detecting and recording layers of volcanic ash in glacier ice, we developed a lightweight, compact probe which uses optical fibers and purely passive downhole components to detect single-scattered long-wavelength light. To characterize ice fabric orientation, we propose a technique which uses reflection measurements from a small, fixed set of geometries. The design and construction of a borehole logger implementing these techniques is described, and its testing discussed.

A Redox-Nucleophilic Dual-Reactable Probe for Highly Selective and Sensitive Detection of H2S: Synthesis, Spectra and Bioimaging

NASA Astrophysics Data System (ADS)

Zhang, Changyu; Wang, Runyu; Cheng, Longhuai; Li, Bingjie; Xi, Zhen; Yi, Long

2016-07-01

Hydrogen sulfide (H2S) is an important signalling molecule with multiple biological functions. The reported H2S fluorescent probes are majorly based on redox or nucleophilic reactions. The combination usage of both redox and nucleophilic reactions could improve the probe’s selectivity, sensitivity and stability. Herein we report a new dual-reactable probe with yellow turn-on fluorescence for H2S detection. The sensing mechanism of the dual-reactable probe was based on thiolysis of NBD (7-nitro-1,2,3-benzoxadiazole) amine (a nucleophilic reaction) and reduction of azide to amine (a redox reaction). Compared with its corresponding single-reactable probes, the dual-reactable probe has higher selectivity and fluorescence turn-on fold with magnitude of multiplication from that of each single-reactable probe. The highly selective and sensitive properties enabled the dual-reactable probe as a useful tool for efficiently sensing H2S in aqueous buffer and in living cells.

Acid-Activatable Michael-Type Fluorescent Probes for Thiols and for Labeling Lysosomes in Live Cells.

PubMed

Dai, Chun-Guang; Du, Xiao-Jiao; Song, Qin-Hua

2015-12-18

A Michael addition is usually taken as a base-catalyzed reaction. Most fluorescent probes have been designed to detect thiols in slightly alkaline solutions (pH 7-9). The sensing reactions of almost all Michael-type fluorescent probes for thiols are faster in a high pH solution than in a low pH solution. In this work, we synthesized a series of 7-substituted 2-(quinolin-2-ylmethylene)malonic acids (QMAs, substituents: NEt2, OH, H, Cl, or NO2) and their ethyl esters (QMEs) as Michael-type fluorescent probes for thiols. The sensing reactions of QMAs and QMEs occur in distinct pH ranges, pH < 7 for QMAs and pH > 7 for QMEs. On the basis of experimental and theoretic studies, we have clarified the distinct pH effects on the sensing reactivity between QMAs and QMEs and demonstrated that two QMAs (NEt2, OH) are highly sensitive and selective fluorescent probes for thiols in acidic solutions (pH < 7) and promising dyes that can label lysosomes in live cells.

Chemical probes for competitive profiling of the quorum sensing signal synthase PqsD of Pseudomonas aeruginosa

PubMed Central

Prothiwa, Michaela; Szamosvári, Dávid; Glasmacher, Sandra

2016-01-01

The human pathogen Pseudomonas aeruginosa uses the pqs quorum sensing system to coordinate the production of its broad spectrum of virulence factors to facilitate colonization and infection of its host. Hereby, the enzyme PqsD is a virulence related quorum sensing signal synthase that catalyzes the central step in the biosynthesis of the Pseudomonas quinolone signals HHQ and PQS. We developed a library of cysteine reactive chemical probes with an alkyne handle for fluorescence tagging and report the selective and highly sensitive in vitro labelling of the active site cysteine of this important enzyme. Interestingly, only one type of probe, with a reactive α-chloroacetamide was capable of covalently reacting with the active site. We demonstrated the potential of our probes in a competitive labelling platform where we screened a library of synthetic HHQ and PQS analogues with heteroatom replacements and found several inhibitors of probe binding that may represent promising scaffolds for the development of customized PqsD inhibitors as well as a chemical toolbox to investigate the activity and active site specificity of the enzyme. PMID:28144351

A Single Electrochemical Probe Used for Analysis of Multiple Nucleic Acid Sequences

PubMed Central

Mills, Dawn M.; Calvo-Marzal, Percy; Pinzon, Jeffer M.; Armas, Stephanie; Kolpashchikov, Dmitry M.; Chumbimuni-Torres, Karin Y.

2017-01-01

Electrochemical hybridization sensors have been explored extensively for analysis of specific nucleic acids. However, commercialization of the platform is hindered by the need for attachment of separate oligonucleotide probes complementary to a RNA or DNA target to an electrode’s surface. Here we demonstrate that a single probe can be used to analyze several nucleic acid targets with high selectivity and low cost. The universal electrochemical four-way junction (4J)-forming (UE4J) sensor consists of a universal DNA stem-loop (USL) probe attached to the electrode’s surface and two adaptor strands (m and f) which hybridize to the USL probe and the analyte to form a 4J associate. The m adaptor strand was conjugated with a methylene blue redox marker for signal ON sensing and monitored using square wave voltammetry. We demonstrated that a single sensor can be used for detection of several different DNA/RNA sequences and can be regenerated in 30 seconds by a simple water rinse. The UE4J sensor enables a high selectivity by recognition of a single base substitution, even at room temperature. The UE4J sensor opens a venue for a re-useable universal platform that can be adopted at low cost for the analysis of DNA or RNA targets. PMID:29371782

Electronic clinical predictive thermometer using logarithm for temperature prediction

NASA Technical Reports Server (NTRS)

Cambridge, Vivien J. (Inventor); Koger, Thomas L. (Inventor); Nail, William L. (Inventor); Diaz, Patrick (Inventor)

1998-01-01

A thermometer that rapidly predicts body temperature based on the temperature signals received from a temperature sensing probe when it comes into contact with the body. The logarithms of the differences between the temperature signals in a selected time frame are determined. A line is fit through the logarithms and the slope of the line is used as a system time constant in predicting the final temperature of the body. The time constant in conjunction with predetermined additional constants are used to compute the predicted temperature. Data quality in the time frame is monitored and if unacceptable, a different time frame of temperature signals is selected for use in prediction. The processor switches to a monitor mode if data quality over a limited number of time frames is unacceptable. Determining the start time on which the measurement time frame for prediction is based is performed by summing the second derivatives of temperature signals over time frames. When the sum of second derivatives in a particular time frame exceeds a threshold, the start time is established.

Optical device for sensing the index of refraction of liquids with high turbidity

NASA Astrophysics Data System (ADS)

Pena-Gomar, M.; Fajardo-Lira, C.; Rosete-Aguilar, Martha; Garcia-Valenzuela, Augusto

2000-12-01

We discuss the use of photo-reflectance near the critical angle (PRCA) to monitor small changes of the RI of highly turbid liquids. The theory of the reflectance of a laser beam near the critical angle for an external medium with a complex RI is summarized. The applicability of PRCA to sense highly turbid media is demonstrated experimentally on bovine milk samples. We give experimental results showing the temporal variation of the refractive index (RI) during three different processes in bovine milk: (1) Mechanical stirring, (2) temperature changes, and (3) pH variations around the isoelectric point of the casein micelles (micelle aggregation). RI changes in the order of a few times 1 X 10-3 are observed during the experiments. The experimental results show that the RI of milk can be used to track physico-chemical changes in time allowing one to measure the time constant of the different process. The design of a compact RI probe for in situ applications is discussed. The miniaturization of such a probe will probably limited by factors other than the loss of sensitivity. A novel angle-of-incidence control which requires only linear displacements of some of the optical components (no rotation) is proposed and shown to be feasible. Such an optical probe may be used in the dairy industry and in general in the food industry or food science research laboratories. It could give additional analytical power to the food scientist, engineer, or technician.

A colorimetric turn-on optical chemosensor for Cu2+ ions and its application as solid state sensor

NASA Astrophysics Data System (ADS)

Pannipara, Mehboobali; Al-Sehemi, Abdullah G.; Assiri, Mohammed; Kalam, Abul

2018-05-01

We report a novel coumarin based optical chemosensor (Probe 1) for the selective and sensitive detection of Cu2+ ions in aqueous medium. The addition of Cu2+ ions to Probe 1 shows distinct color change from light yellow to pinkish red color under visible light with the sensing limit of 1.54 μM. Moreover, practical utility of Probe 1 as solid state optical sensor (test paper, TLC plates) for sensing Cu2+ has been demonstrated by instantaneous "naked eye" response.

Femtomolar level sensing of inorganic arsenic(III) in water and in living-systems using a non-toxic fluorescent probe.

PubMed

Dey, Biswajit; Mukherjee, Priyanka; Mondal, Ranjan Kumar; Chattopadhyay, Asoke Prasun; Hauli, Ipsit; Mukhopadhyay, Subhra Kanti; Fleck, Michel

2014-12-14

A highly selective femtomolar level sensing of inorganic arsenic(III) as arsenious acid has been accomplished in water medium and in living-systems (on pollen grains of Tecoma stans; Candida albicans cells (IMTECH No. 3018) and Peperomia pellucida stem section) using a non-toxic fluorescent probe of a Cu(II)-complex.

High Temperature Ultrasonic Probe and Pulse-Echo Probe Mounting Fixture for Testing and Blind Alignment on Steam Pipes

NASA Technical Reports Server (NTRS)

Lih, Shyh-Shiuh (Inventor); Takano, Nobuyuki (Inventor); Lee, Hyeong Jae (Inventor); Bao, Xiaoqi (Inventor); Badescu, Mircea (Inventor); Bar-Cohen, Yoseph (Inventor); Sherrit, Stewart (Inventor); Ostlund, Patrick N. (Inventor)

2017-01-01

A high temperature ultrasonic probe and a mounting fixture for attaching and aligning the probe to a steam pipe using blind alignment. The high temperature ultrasonic probe includes a piezoelectric transducer having a high temperature. The probe provides both transmitting and receiving functionality. The mounting fixture allows the high temperature ultrasonic probe to be accurately aligned to the bottom external surface of the steam pipe so that the presence of liquid water in the steam pipe can be monitored. The mounting fixture with a mounted high temperature ultrasonic probe are used to conduct health monitoring of steam pipes and to track the height of condensed water through the wall in real-time.

A new temperature profiling probe for investigating groundwater-surface water interaction

USGS Publications Warehouse

Naranjo, Ramon C.; Robert Turcotte,

2015-01-01

Measuring vertically nested temperatures at the streambed interface poses practical challenges that are addressed here with a new discrete subsurface temperature profiling probe. We describe a new temperature probe and its application for heat as a tracer investigations to demonstrate the probe's utility. Accuracy and response time of temperature measurements made at 6 discrete depths in the probe were analyzed in the laboratory using temperature bath experiments. We find the temperature probe to be an accurate and robust instrument that allows for easily installation and long-term monitoring in highly variable environments. Because the probe is inexpensive and versatile, it is useful for many environmental applications that require temperature data collection for periods of several months in environments that are difficult to access or require minimal disturbance.

Miniature fibre optic probe for minimally invasive photoacoustic sensing

NASA Astrophysics Data System (ADS)

Mathews, Sunish J.; Zhang, Edward Z.; Desjardins, Adrien E.; Beard, Paul C.

2016-03-01

A miniature (175 μm) all-optical photoacoustic probe has been developed for minimally invasive sensing and imaging applications. The probe comprises a single optical fibre which delivers the excitation light and a broadband 50 MHz Fabry-Pérot (F-P) ultrasound sensor at the distal end for detecting the photoacoustic waves. A graded index lens proximal to the F-P sensor is used to reduce beam walk-off and thus increase sensitivity as well as confine the excitation beam in order to increase lateral spatial resolution. The probe was evaluated in non-scattering media and found to provide lateral and axial resolutions of < 100 μm and < 150 μm respectively for distances up to 1 cm from the tip of the probe. The ability of the probe to detect a blood vessel mimicking phantom at distances up to 7 mm from the tip was demonstrated in order to illustrate its potential suitability for needle guidance applications.

Direct Sequence Detection of Structured H5 Influenza Viral RNA

PubMed Central

Kerby, Matthew B.; Freeman, Sarah; Prachanronarong, Kristina; Artenstein, Andrew W.; Opal, Steven M.; Tripathi, Anubhav

2008-01-01

We describe the development of sequence-specific molecular beacons (dual-labeled DNA probes) for identification of the H5 influenza subtype, cleavage motif, and receptor specificity when hybridized directly with in vitro transcribed viral RNA (vRNA). The cloned hemagglutinin segment from a highly pathogenic H5N1 strain, A/Hanoi/30408/2005(H5N1), isolated from humans was used as template for in vitro transcription of sense-strand vRNA. The hybridization behavior of vRNA and a conserved subtype probe was characterized experimentally by varying conditions of time, temperature, and Mg2+ to optimize detection. Comparison of the hybridization rates of probe to DNA and RNA targets indicates that conformational switching of influenza RNA structure is a rate-limiting step and that the secondary structure of vRNA dominates the binding kinetics. The sensitivity and specificity of probe recognition of other H5 strains was calculated from sequence matches to the National Center for Biotechnology Information influenza database. The hybridization specificity of the subtype probes was experimentally verified with point mutations within the probe loop at five locations corresponding to the other human H5 strains. The abundance frequencies of the hemagglutinin cleavage motif and sialic acid recognition sequences were experimentally tested for H5 in all host viral species. Although the detection assay must be coupled with isothermal amplification on the chip, the new probes form the basis of a portable point-of-care diagnostic device for influenza subtyping. PMID:18403607

Sensing Performance Analysis on Quartz Tuning Fork-Probe at the High Order Vibration Mode for Multi-Frequency Scanning Probe Microscopy

PubMed Central

Gao, Fengli; Li, Xide

2018-01-01

Multi-frequency scanning near-field optical microscopy, based on a quartz tuning fork-probe (QTF-p) sensor using the first two orders of in-plane bending symmetrical vibration modes, has recently been developed. This method can simultaneously achieve positional feedback (based on the 1st in-plane mode called the low mode) and detect near-field optically induced forces (based on the 2nd in-plane mode called the high mode). Particularly, the high mode sensing performance of the QTF-p is an important issue for characterizing the tip-sample interactions and achieving higher resolution microscopic imaging but the related researches are insufficient. Here, we investigate the vibration performance of QTF-p at high mode based on the experiment and finite element method. The frequency spectrum characteristics are obtained by our homemade laser Doppler vibrometer system. The effects of the properties of the connecting glue layer and the probe features on the dynamic response of the QTF-p sensor at the high mode are investigated for optimization design. Finally, compared with the low mode, an obvious improvement of quality factor, of almost 50%, is obtained at the high mode. Meanwhile, the QTF-p sensor has a high force sensing sensitivity and a large sensing range at the high mode, indicating a broad application prospect for force sensing. PMID:29364847

Experimental study of the evanescent-wave photonic sensors response in presence of molecular beacon conformational changes.

PubMed

Ruiz-Tórtola, Ángela; Prats-Quílez, Francisco; Gónzalez-Lucas, Daniel; Bañuls, María-José; Maquieira, Ángel; Wheeler, Guy; Dalmay, Tamas; Griol, Amadeu; Hurtado, Juan; Bohlmann, Helge; Götzen, Reiner; García-Rupérez, Jaime

2018-04-17

An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probes -upon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensors- is reported. To this end, high sensitivity photonic sensors based on silicon photonic bandgap (PBG) structures were used, where the MB probes were immobilized via their 5' termination. Those MBs incorporate a biotin moiety close to their 3' termination in order to selectively bind a streptavidin molecule to them. The different photonic sensing responses obtained towards the target oligonucleotide detection, when the streptavidin molecule was bound to the MB probes or not, demonstrate the conformational change suffered by the MB upon hybridization, which promotes the displacement of the streptavidin molecule away from the surface of the photonic sensing structure. Schematic diagram of the PBG sensing structure on which the streptavidin-labeled MB probes were immobilized. This article is protected by copyright. All rights reserved.

Cellular Oxygen and Nutrient Sensing in Microgravity Using Time-Resolved Fluorescence Microscopy

NASA Technical Reports Server (NTRS)

Szmacinski, Henryk

2003-01-01

Oxygen and nutrient sensing is fundamental to the understanding of cell growth and metabolism. This requires identification of optical probes and suitable detection technology without complex calibration procedures. Under this project Microcosm developed an experimental technique that allows for simultaneous imaging of intra- and inter-cellular events. The technique consists of frequency-domain Fluorescence Lifetime Imaging Microscopy (FLIM), a set of identified oxygen and pH probes, and methods for fabrication of microsensors. Specifications for electronic and optical components of FLIM instrumentation are provided. Hardware and software were developed for data acquisition and analysis. Principles, procedures, and representative images are demonstrated. Suitable lifetime sensitive oxygen, pH, and glucose probes for intra- and extra-cellular measurements of analyte concentrations have been identified and tested. Lifetime sensing and imaging have been performed using PBS buffer, culture media, and yeast cells as a model systems. Spectral specifications, calibration curves, and probes availability are also provided in the report.

Simultaneous sensing of temperature, CO, and CO2 in a scramjet combustor using quantum cascade laser absorption spectroscopy

NASA Astrophysics Data System (ADS)

Spearrin, R. M.; Goldenstein, C. S.; Schultz, I. A.; Jeffries, J. B.; Hanson, R. K.

2014-07-01

A mid-infrared laser absorption sensor was developed for gas temperature and carbon oxide (CO, CO2) concentrations in high-enthalpy, hydrocarbon combustion flows. This diagnostic enables non-intrusive, in situ measurements in harsh environments produced by hypersonic propulsion ground test facilities. The sensing system utilizes tunable quantum cascade lasers capable of probing the fundamental mid-infrared absorption bands of CO and CO2 in the 4-5 µm wavelength domain. A scanned-wavelength direct absorption technique was employed with two lasers, one dedicated to each species, free-space fiber-coupled using a bifurcated hollow-core fiber for remote light delivery on a single line of sight. Scanned-wavelength modulation spectroscopy with second-harmonic detection was utilized to extend the dynamic range of the CO measurement. The diagnostic was field-tested on a direct-connect scramjet combustor for ethylene-air combustion. Simultaneous, laser-based measurements of carbon monoxide and carbon dioxide provide a basis for evaluating combustion completion or efficiency with temporal and spatial resolution in practical hydrocarbon-fueled engines.

Quantum control and engineering of single spins in diamond

NASA Astrophysics Data System (ADS)

Toyli, David M.

The past two decades have seen intensive research efforts aimed at creating quantum technologies that leverage phenomena such as coherence and entanglement to achieve device functionalities surpassing those attainable with classical physics. While the range of applications for quantum devices is typically limited by their cryogenic operating temperatures, in recent years point defects in semiconductors have emerged as potential candidates for room temperature quantum technologies. In particular, the nitrogen vacancy (NV) center in diamond has gained prominence for the ability to measure and control its spin under ambient conditions and for its potential applications in magnetic sensing. Here we describe experiments that probe the thermal limits to the measurement and control of single NV centers to identify the origin of the system's unique temperature dependence and that define novel thermal sensing applications for single spins. We demonstrate the optical measurement and coherent control of the spin at temperatures exceeding 600 K and show that its addressability is eventually limited by thermal quenching of the optical spin readout. These measurements provide important information for the electronic structure responsible for the optical spin initialization and readout processes and, moreover, suggest that the coherence of the NV center's spin states could be harnessed for thermometry applications. To that end, we develop novel quantum control techniques that selectively probe thermally induced shifts in the spin resonance frequencies while minimizing the defect's interactions with nearby nuclear spins. We use these techniques to extend the NV center's spin coherence for thermometry by 45-fold to achieve thermal sensitivities approaching 10 mK Hz-1/2 . We show the versatility of these techniques by performing measurements in a range of magnetic environments and at temperatures as high as 500 K. Together with diamond's ideal thermal, mechanical, and chemical properties, these measurements suggest that NV center sensors could be employed in a diverse range of applications such as intracellular thermometry, microfuidic thermometry, and scanning thermal microscopy. Finally, while the development of NV center technologies is motivated by the desirable properties of isolated defects in bulk diamond, the realization of many of these technologies, such as those using the spin as a proximal sensor, require a means to control the placement of NV centers within the diamond lattice. We demonstrate a method to pattern defect formation on sub-100-nm length scales using ion implantation and electron beam lithography techniques. The ability to engineer large scale arrays of NV centers with this method holds promise for a variety of applications in quantum information science and nanoscale sensing.

Fluorescent Protein Voltage Probes Derived from ArcLight that Respond to Membrane Voltage Changes with Fast Kinetics

PubMed Central

Han, Zhou; Jin, Lei; Platisa, Jelena; Cohen, Lawrence B.; Baker, Bradley J.; Pieribone, Vincent A.

2013-01-01

We previously reported the discovery of a fluorescent protein voltage probe, ArcLight, and its derivatives that exhibit large changes in fluorescence intensity in response to changes of plasma membrane voltage. ArcLight allows the reliable detection of single action potentials and sub-threshold activities in individual neurons and dendrites. The response kinetics of ArcLight (τ1-on ~10 ms, τ2-on ~ 50 ms) are comparable with most published genetically-encoded voltage probes. However, probes using voltage-sensing domains other than that from the Ciona intestinalis voltage sensitive phosphatase exhibit faster kinetics. Here we report new versions of ArcLight, in which the Ciona voltage-sensing domain was replaced with those from chicken, zebrafish, frog, mouse or human. We found that the chicken and zebrafish-based ArcLight exhibit faster kinetics, with a time constant (τ) less than 6ms for a 100 mV depolarization. Although the response amplitude of these two probes (8-9%) is not as large as the Ciona-based ArcLight (~35%), they are better at reporting action potentials from cultured neurons at higher frequency. In contrast, probes based on frog, mouse and human voltage sensing domains were either slower than the Ciona-based ArcLight or had very small signals. PMID:24312287

RETRACTED ARTICLE: Quasi-distributed fiber bragg grating array sensor for furnace applications

NASA Astrophysics Data System (ADS)

Reddy, P. Saidi; Sai Prasad, R. L. N.; Sen Gupta, D.; Sai Shankar, M.; Srimannarayana, K.; Ravinder Reddy, P.

2012-05-01

An experimental work on distributed temperature sensing making use of the fiber Bragg grating (FBG) array sensor for possible applications in the monitoring of the temperature profile in high temperature boilers is presented. A special sensor has been designed for this purpose which consists of four FBGs (of wavelengths λ B1 =1545.8 nm, λ B2 =1547 nm, λ B3 =1550.8 nm, λ B4 =1555.5 nm at 30 °C) written in the hydrogen-loaded fiber in line. All the FBGs are encapsulated inside a stainless steel tube using the rigid probe technique for avoiding micro cracks. The spatial distribution of the temperature profile inside a prototype boiler was measured experimentally both in horizontal and vertical directions employing the above sensor, and the results are presented. Further, the finite element simulation has been carried out by using ANSYS R11 software to predict temperature contours in the boiler, and the experimental and predicted results were found to be closely matching.

Detection of target-probe oligonucleotide hybridization using synthetic nanopore resistive pulse sensing.

PubMed

Booth, Marsilea Adela; Vogel, Robert; Curran, James M; Harbison, SallyAnn; Travas-Sejdic, Jadranka

2013-07-15

Despite the plethora of DNA sensor platforms available, a portable, sensitive, selective and economic sensor able to rival current fluorescence-based techniques would find use in many applications. In this research, probe oligonucleotide-grafted particles are used to detect target DNA in solution through a resistive pulse nanopore detection technique. Using carbodiimide chemistry, functionalized probe DNA strands are attached to carboxylated dextran-based magnetic particles. Subsequent incubation with complementary target DNA yields a change in surface properties as the two DNA strands hybridize. Particle-by-particle analysis with resistive pulse sensing is performed to detect these changes. A variable pressure method allows identification of changes in the surface charge of particles. As proof-of-principle, we demonstrate that target hybridization is selectively detected at micromolar concentrations (nanomoles of target) using resistive pulse sensing, confirmed by fluorescence and phase analysis light scattering as complementary techniques. The advantages, feasibility and limitations of using resistive pulse sensing for sample analysis are discussed. Copyright © 2013 Elsevier B.V. All rights reserved.

Single-Crystal Sapphire Optical Fiber Sensor Instrumentation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pickrell, Gary; Scott, Brian; Wang, Anbo

2013-12-31

This report summarizes technical progress on the program “Single-Crystal Sapphire Optical Fiber Sensor Instrumentation,” funded by the National Energy Technology Laboratory of the U.S. Department of Energy, and performed by the Center for Photonics Technology of the Bradley Department of Electrical and Computer Engineering at Virginia Tech. This project was completed in three phases, each with a separate focus. Phase I of the program, from October 1999 to April 2002, was devoted to development of sensing schema for use in high temperature, harsh environments. Different sensing designs were proposed and tested in the laboratory. Phase II of the program, frommore » April 2002 to April 2009, focused on bringing the sensor technologies, which had already been successfully demonstrated in the laboratory, to a level where the sensors could be deployed in harsh industrial environments and eventually become commercially viable through a series of field tests. Also, a new sensing scheme was developed and tested with numerous advantages over all previous ones in Phase II. Phase III of the program, September 2009 to December 2013, focused on development of the new sensing scheme for field testing in conjunction with materials engineering of the improved sensor packaging lifetimes. In Phase I, three different sensing principles were studied: sapphire air-gap extrinsic Fabry-Perot sensors; intensity-based polarimetric sensors; and broadband polarimetric sensors. Black body radiation tests and corrosion tests were also performed in this phase. The outcome of the first phase of this program was the selection of broadband polarimetric differential interferometry (BPDI) for further prototype instrumentation development. This approach is based on the measurement of the optical path difference (OPD) between two orthogonally polarized light beams in a single-crystal sapphire disk. At the beginning of Phase II, in June 2004, the BPDI sensor was tested at the Wabash River coal gasifier facility in Terre Haute, Indiana. Due to business conditions at industrial partner and several logistical problems, this field test was not successful. An alternative high-temperature sensing system using sapphire wafer-based extrinsic Fabry-Perot interferometry was then developed as a significant improvement over the BPDI solution. From June 2006 to June 2008, three consecutive field tests were performed with the new sapphire wafer sensors at the TECO coal gasifier in Tampa, Florida. One of the sensors survived in the industrial coal gasifier for 7 months, over which time the existing thermocouples were replaced twice. The outcome of these TECO field tests suggests that the sapphire wafer sensor has very good potential to be commercialized. However packaging and sensor protection issues need additional development. During Phase III, several major improvements in the design and fabrication process of the sensor have been achieved through experiments and theoretical analysis. Studies on the property of the key components in the sensor head, including the sapphire fiber and sapphire wafer, were also conducted, for a better understanding of the sensor behavior. A final design based on all knowledge and experience has been developed, free of any issues encountered during the entire research. Sensors with this design performed well as expected in lab long-term tests, and were deployed in the sensing probe of the final coal-gasifier field test. Sensor packaging and protection was improved through materials engineering through testing of packaging designs in two blank probe packaging tests at Eastman Chemical in Kingsport, TN. Performance analysis of the blank probe packaging resulted in improve package designs culminating in a 3rd generation probe packaging utilized for the full field test of the sapphire optical sensor and materials designed sensor packaging.« less

Measurements of Turbulent Kinetic Energy Dissipation Rate in Complex Terrain from the Perdigão Experiment

NASA Astrophysics Data System (ADS)

Lundquist, J. K.; Bariteau, L.; Hocut, C. M.; Creegan, E.; Chow, F. K.; Mazzaro, L.; Bodini, N.; Tomaszewski, J.; Murphy, P.; Neher, J.; van Veen, L.; Pattantyus, A.

2017-12-01

To investigate the turbulent mixing events within and around Portugal's Perdigão valley during the intensive field campaign of 1 May - 15 June 2017, the University of Colorado Boulder (CU) and the Army Research Laboratory (ARL) deployed Tethered Lifting Systems (TLS). The TLS carried turbulence probes to sample from the ground to 500 m above the surface. These probes sample temperature, relative humidity, and winds, including very high-rate measurements that enable measurement of turbulence dissipation rate and temperature structure parameters. The vertical resolution of the systems is defined by the ascent and descent rates of the probes, making this platform uniquely suited for sensing and characterizng stratification and layering undetectable by other instrumentation in the campaign. Therefore, the detailed information provided by the TLS (see Figure 1) allows us to characterize the intermittent turbulence events and circulations above and within the valley with detail sufficient for evaluation of mesoscale-microscale modeling of these flows. This presentation will survey the datasets collected by the University of Colorado's TLS located in the center of the valley. Specific case studies will be highlighted, including turbine wake measurements, interactions between drainage flow down-valley and mesoscale forcing up-valley, evening transitions, and morning transitions. Several coordinated "flights" of the CU and ARL systems within and outside of the valley enabled contrasting measurements to provide insight into the terrain-driven mixing events.

A rapid and selective fluorescent probe with a large Stokes shift for the detection of hydrogen sulfide.

PubMed

Chen, Song; Hou, Peng; Wang, Jing; Fu, Shuang; Liu, Lei

2018-05-28

We have successfully developed a new green-emitting H 2 S fluorescence probe employing a 2,4-dinitrophenyl ether moiety as the sensing group based on 3'-formyl-4'-hydroxybiphenyl-4-carbonitrile. This probe displayed a rapid (2 min), sensitive (the detection limit was 0.18 μM) and selective with a large Stokes shift (183 nm) in response to H 2 S, which was beneficial for fluorescence sensing and cell imaging studies. Moreover, this probe can qualitatively and quantitatively detect H 2 S with a good linearity (R 2  = 0.9991). Importantly, this probe had been used for the detection of H 2 S in living MDA-MB-231 cells with good performance. Copyright © 2018 Elsevier B.V. All rights reserved.

Atomic magnetometer

DOEpatents

Schwindt, Peter [Albuquerque, NM; Johnson, Cort N [Albuquerque, NM

2012-07-03

An atomic magnetometer is disclosed which uses a pump light beam at a D1 or D2 transition of an alkali metal vapor to magnetically polarize the vapor in a heated cell, and a probe light beam at a different D2 or D1 transition to sense the magnetic field via a polarization rotation of the probe light beam. The pump and probe light beams are both directed along substantially the same optical path through an optical waveplate and through the heated cell to an optical filter which blocks the pump light beam while transmitting the probe light beam to one or more photodetectors which generate electrical signals to sense the magnetic field. The optical waveplate functions as a quarter waveplate to circularly polarize the pump light beam, and as a half waveplate to maintain the probe light beam linearly polarized.

Dynamic Pressure Probes Developed for Supersonic Flow-Field Measurements

NASA Technical Reports Server (NTRS)

Porro, A. Robert

2001-01-01

A series of dynamic flow-field pressure probes were developed for use in large-scale supersonic wind tunnels at the NASA Glenn Research Center. These flow-field probes include pitot and static pressure probes that can capture fast-acting flow-field pressure transients occurring on a millisecond timescale. The pitot and static probes can be used to determine local Mach number time histories during a transient event. The flow-field pressure probe contains four major components: 1) Static pressure aerodynamic tip; 2) Pressure-sensing cartridge assembly; 3) Pitot pressure aerodynamic tip; 4) Mounting stem. This modular design allows for a variety of probe tips to be used for a specific application. Here, the focus is on flow-field pressure measurements in supersonic flows, so we developed a cone-cylinder static pressure tip and a pitot pressure tip. Alternatively, probe tips optimized for subsonic and transonic flows could be used with this design. The pressure-sensing cartridge assembly allows the simultaneous measurement of steady-state and transient pressure which allows continuous calibration of the dynamic pressure transducer.

Real time sensor for therapeutic radiation delivery

DOEpatents

Bliss, M.; Craig, R.A.; Reeder, P.L.

1998-01-06

The invention is a real time sensor for therapeutic radiation. A probe is placed in or near the patient that senses in real time the dose at the location of the probe. The strength of the dose is determined by either an insertion or an exit probe. The location is determined by a series of vertical and horizontal sensing elements that gives the operator a real time read out dose location relative to placement of the patient. The increased accuracy prevents serious tissue damage to the patient by preventing overdose or delivery of a dose to a wrong location within the body. 14 figs.

Real time sensor for therapeutic radiation delivery

DOEpatents

Bliss, Mary; Craig, Richard A.; Reeder, Paul L.

1998-01-01

The invention is a real time sensor for therapeutic radiation. A probe is placed in or near the patient that senses in real time the dose at the location of the probe. The strength of the dose is determined by either an insertion or an exit probe. The location is determined by a series of vertical and horizontal sensing elements that gives the operator a real time read out dose location relative to placement of the patient. The increased accuracy prevents serious tissue damage to the patient by preventing overdose or delivery of a dose to a wrong location within the body.

Developing and bounding ice particle mass- and area-dimension expressions for use in atmospheric models and remote sensing

DOE PAGES

Erfani, Ehsan; Mitchell, David L.

2016-04-07

Here, ice particle mass- and projected area-dimension ( m- D and A- D) power laws are commonly used in the treatment of ice cloud microphysical and optical properties and the remote sensing of ice cloud properties. Although there has long been evidence that a single m- D or A- D power law is often not valid over all ice particle sizes, few studies have addressed this fact. This study develops self-consistent m- D and A- D expressions that are not power laws but can easily be reduced to power laws for the ice particle size (maximum dimension or D) rangemore » of interest, and they are valid over a much larger D range than power laws. This was done by combining ground measurements of individual ice particle m and D formed at temperature T < –20 °C during a cloud seeding field campaign with 2-D stereo (2D-S) and cloud particle imager (CPI) probe measurements of D and A, and estimates of m, in synoptic and anvil ice clouds at similar temperatures. The resulting m- D and A- D expressions are functions of temperature and cloud type (synoptic vs. anvil), and are in good agreement with m- D power laws developed from recent field studies considering the same temperature range (–60 °C < T < –20 °C).« less

Oxygen Sensing, Hypoxia Tracing and in Vivo Imaging with Functional Metalloprobes for the Early Detection of Non-communicable Diseases

PubMed Central

Mirabello, Vincenzo; Cortezon-Tamarit, Fernando; Pascu, Sofia I.

2018-01-01

Hypoxia has been identified as one of the hallmarks of tumor environments and a prognosis factor in many cancers. The development of ideal chemical probes for imaging and sensing of hypoxia remains elusive. Crucial characteristics would include a measurable response to subtle variations of pO2 in living systems and an ability to accumulate only in the areas of interest (e.g., targeting hypoxia tissues) whilst exhibiting kinetic stabilities in vitro and in vivo. A sensitive probe would comprise platforms for applications in imaging and therapy for non-communicable diseases (NCDs) relying on sensitive detection of pO2. Just a handful of probes for the in vivo imaging of hypoxia [mainly using positron emission tomography (PET)] have reached the clinical research stage. Many chemical compounds, whilst presenting promising in vitro results as oxygen-sensing probes, are facing considerable disadvantages regarding their general application in vivo. The mechanisms of action of many hypoxia tracers have not been entirely rationalized, especially in the case of metallo-probes. An insight into the hypoxia selectivity mechanisms can allow an optimization of current imaging probes candidates and this will be explored hereby. The mechanistic understanding of the modes of action of coordination compounds under oxygen concentration gradients in living cells allows an expansion of the scope of compounds toward in vivo applications which, in turn, would help translate these into clinical applications. We summarize hereby some of the recent research efforts made toward the discovery of new oxygen sensing molecules having a metal-ligand core. We discuss their applications in vitro and/or in vivo, with an appreciation of a plethora of molecular imaging techniques (mainly reliant on nuclear medicine techniques) currently applied in the detection and tracing of hypoxia in the preclinical and clinical setups. The design of imaging/sensing probe for early-stage diagnosis would longer term avoid invasive procedures providing platforms for therapy monitoring in a variety of NCDs and, particularly, in cancers. PMID:29527524

High Surface Area MoS 2/Graphene Hybrid Aerogel for Ultrasensitive NO 2 Detection

DOE PAGES

Long, Hu; Harley-Trochimczyk, Anna; Pham, Thang; ...

2016-05-23

A MoS 2/graphene hybrid aerogel synthesized with two-dimensional MoS 2 sheets coating a high surface area graphene aerogel scaffold is characterized and used for ultrasensitive NO 2 detection. The combination of graphene and MoS 2 leads to improved sensing properties with the graphene scaffold providing high specific surface area and high electrical and thermal conductivity and the single to few-layer MoS2 sheets providing high sensitivity and selectivity to NO 2. The hybrid aerogel is integrated onto a low-power microheater platform to probe the gas sensing performance. At room temperature, the sensor exhibits an ultralow detection limit of 50 ppb NOmore » 2. By heating the material to 200 °C, the response and recovery times to reach 90% of the final signal decrease to <1 min, while retaining the low detection limit. The MoS 2/graphene hybrid also shows good selectivity for NO 2 against H 2 and CO, especially when compared to bare graphene aerogel. The unique structure of the hybrid aerogel is responsible for the ultrasensitive, selective, and fast NO 2 sensing. The improved sensing performance of this hybrid aerogel also suggests the possibility of other 2D material combinations for further sensing applications.« less

Non-Covalent Fluorescent Labeling of Hairpin DNA Probe Coupled with Hybridization Chain Reaction for Sensitive DNA Detection.

PubMed

Song, Luna; Zhang, Yonghua; Li, Junling; Gao, Qiang; Qi, Honglan; Zhang, Chengxiao

2016-04-01

An enzyme-free signal amplification-based assay for DNA detection was developed using fluorescent hairpin DNA probes coupled with hybridization chain reaction (HCR). The hairpin DNAs were designed to contain abasic sites in the stem moiety. Non-covalent labeling of the hairpin DNAs was achieved when a fluorescent ligand was bound to the abasic sites through hydrogen bonding with the orphan cytosine present on the complementary strand, accompanied by quench of ligand fluorescence. As a result, the resultant probes, the complex formed between the hairpin DNA and ligand, showed almost no fluorescence. Upon hybridization with target DNA, the probe underwent a dehybridization of the stem moiety containing an abasic site. The release of ligand from the abasic site to the solution resulted in an effective fluorescent enhancement, which can be used as a signal. Compared with a sensing system without HCR, a 20-fold increase in the sensitivity was achieved using the sensing system with HCR. The fluorescent intensity of the sensing system increased with the increase in target DNA concentration from 0.5 nM to 100 nM. A single mismatched target ss-DNA could be effectively discriminated from complementary target DNA. Genotyping of a G/C single-nucleotide polymorphism of polymerase chain reaction (PCR) products was successfully demonstrated with the sensing system. Therefore, integrating HCR strategy with non-covalent labeling of fluorescent hairpin DNA probes provides a sensitive and cost-effective DNA assay. © The Author(s) 2016.

Thermal Characterization of a Simulated Fission Engine via Distributed Fiber Bragg Gratings

NASA Astrophysics Data System (ADS)

Duncan, Roger G.; Fielder, Robert S.; Seeley, Ryan J.; Kozikowski, Carrie L.; Raum, Matthew T.

2005-02-01

We report the use of distributed fiber Bragg gratings to monitor thermal conditions within a simulated nuclear reactor core located at the Early Flight Fission Test Facility of the NASA Marshall Space Flight Center. Distributed fiber-optic temperature measurements promise to add significant capability and advance the state-of-the-art in high-temperature sensing. For the work reported herein, seven probes were constructed with ten sensors each for a total of 70 sensor locations throughout the core. These discrete temperature sensors were monitored over a nine hour period while the test article was heated to over 700 °C and cooled to ambient through two operational cycles. The sensor density available permits a significantly elevated understanding of thermal effects within the simulated reactor. Fiber-optic sensor performance is shown to compare very favorably with co-located thermocouples where such co-location was feasible.

The Scanning Theremin Microscope: A Model Scanning Probe Instrument for Hands-On Activities

ERIC Educational Resources Information Center

Quardokus, Rebecca C.; Wasio, Natalie A.; Kandel, S. Alex

2014-01-01

A model scanning probe microscope, designed using similar principles of operation to research instruments, is described. Proximity sensing is done using a capacitance probe, and a mechanical linkage is used to scan this probe across surfaces. The signal is transduced as an audio tone using a heterodyne detection circuit analogous to that used in…

An intramolecular crossed-benzoin reaction based KCN fluorescent probe in aqueous and biological environments.

PubMed

Lee, Jae Hong; Jang, Joo Hee; Velusamy, Nithya; Jung, Hyo Sung; Bhuniya, Sankarprasad; Kim, Jong Seung

2015-05-04

A turn-on fluorescent probe was designed for selective cyanide anion sensing in aqueous and biological environments. The probe underwent an intramolecular crossed-benzoin reaction in the presence of KCN to expel the fluorophore resorufin. This probe was sensitive to KCN concentrations as low as 4 nM in aqueous media.

A cost-effective quasi-distributed liquid leakage sensor based on the polymer optical fiber and flexible lamp belt with LEDs.

PubMed

Zhang, Yingzi; Hou, Yulong; Zhang, Yanjun; Hu, Yanjun; Zhang, Liang; Gao, Xiaolong; Zhang, Huixin; Liu, Wenyi

2018-04-16

A quasi-distributed liquid leakage (QDLL) sensor in local area is proposed and experimentally demonstrated, providing a real-time yet low-cost method than the existing local QDLL sensor. The sensor mainly consists of a flexible lamp belt (FLB) with light-emitting diodes (LEDs) and a polymer optical fiber (POF) processed with side-coupling structures. The side-coupling structures are illuminated by the LEDs one by one, forming a series of sensing probes. The lights are side-coupled into the POF through the side-coupling structure and pulse sequences are obtained from the power meters connected to the both ends of the POF. Each pulse represents a sensing probe, and the intensity of them increase when the coupling medium changes from air to liquid. The location of the leakage incident can be got by the position of each pulse in its output sequence. The influence of different side-coupling structures on side-coupling ratio are investigated. The experiment results validate the detection and localization abilities of the QDLL sensor along a 1 m-long POF with a spatial resolution of 0.1 m, which can be improved by adjusting the side-coupling structure. Furthermore, the temperature dependence is studied and can be compensated.

Research on Dynamic Torque Measurement of High Speed Rotating Axis Based on Whole Optical Fiber Technique

NASA Astrophysics Data System (ADS)

Ma, H. P.; Jin, Y. Q.; Ha, Y. W.; Liu, L. H.

2006-10-01

Non-contact torque measurement system of fiber grating is proposed in this paper. It is used for the dynamic torque measurement of the rotating axis in the spaceflight servo system. Optical fiber is used as sensing probe with high sensitivity, anti-electromagnetic interference, resistance to high temperature and corrosion. It is suitable to apply in a bad environment. Signals are processed by digital circuit and Single Chip Microcomputer. This project can realize super speed dynamic measurement and it is the first time to apply the project in the spaceflight system.

Highlights in the study of exoplanet atmospheres

NASA Astrophysics Data System (ADS)

Burrows, Adam S.

2014-09-01

Exoplanets are now being discovered in profusion. To understand their character, however, we require spectral models and data. These elements of remote sensing can yield temperatures, compositions and even weather patterns, but only if significant improvements in both the parameter retrieval process and measurements are made. Despite heroic efforts to garner constraining data on exoplanet atmospheres and dynamics, reliable interpretation has frequently lagged behind ambition. I summarize the most productive, and at times novel, methods used to probe exoplanet atmospheres; highlight some of the most interesting results obtained; and suggest various broad theoretical topics in which further work could pay significant dividends.

Thermodynamically optimal whole-genome tiling microarray design and validation.

PubMed

Cho, Hyejin; Chou, Hui-Hsien

2016-06-13

Microarray is an efficient apparatus to interrogate the whole transcriptome of species. Microarray can be designed according to annotated gene sets, but the resulted microarrays cannot be used to identify novel transcripts and this design method is not applicable to unannotated species. Alternatively, a whole-genome tiling microarray can be designed using only genomic sequences without gene annotations, and it can be used to detect novel RNA transcripts as well as known genes. The difficulty with tiling microarray design lies in the tradeoff between probe-specificity and coverage of the genome. Sequence comparison methods based on BLAST or similar software are commonly employed in microarray design, but they cannot precisely determine the subtle thermodynamic competition between probe targets and partially matched probe nontargets during hybridizations. Using the whole-genome thermodynamic analysis software PICKY to design tiling microarrays, we can achieve maximum whole-genome coverage allowable under the thermodynamic constraints of each target genome. The resulted tiling microarrays are thermodynamically optimal in the sense that all selected probes share the same melting temperature separation range between their targets and closest nontargets, and no additional probes can be added without violating the specificity of the microarray to the target genome. This new design method was used to create two whole-genome tiling microarrays for Escherichia coli MG1655 and Agrobacterium tumefaciens C58 and the experiment results validated the design.

Highly selective detection of single-nucleotide polymorphisms using a quartz crystal microbalance biosensor based on the toehold-mediated strand displacement reaction.

PubMed

Wang, Dingzhong; Tang, Wei; Wu, Xiaojie; Wang, Xinyi; Chen, Gengjia; Chen, Qiang; Li, Na; Liu, Feng

2012-08-21

Toehold-mediated strand displacement reaction (SDR) is first introduced to develop a simple quartz crystal microbalance (QCM) biosensor without an enzyme or label at normal temperature for highly selective and sensitive detection of single-nucleotide polymorphism (SNP) in the p53 tumor suppressor gene. A hairpin capture probe with an external toehold is designed and immobilized on the gold electrode surface of QCM. A successive SDR is initiated by the target sequence hybridization with the toehold domain and ends with the unfolding of the capture probe. Finally, the open-loop capture probe hybridizes with the streptavidin-coupled reporter probe as an efficient mass amplifier to enhance the QCM signal. The proposed biosensor displays remarkable specificity to target the p53 gene fragment against single-base mutant sequences (e.g., the largest discrimination factor is 63 to C-C mismatch) and high sensitivity with the detection limit of 0.3 nM at 20 °C. As the crucial component of the fabricated biosensor for providing the high discrimination capability, the design rationale of the capture probe is further verified by fluorescence sensing and atomic force microscopy imaging. Additionally, a recovery of 84.1% is obtained when detecting the target sequence in spiked HeLa cells lysate, demonstrating the feasibility of employing this biosensor in detecting SNPs in biological samples.

Remote atmospheric probing by ground to ground line of sight optical methods

NASA Technical Reports Server (NTRS)

Lawrence, R. S.

1969-01-01

The optical effects arising from refractive-index variations in the clear air are qualitatively described, and the possibilities are discussed of using those effects for remotely sensing the physical properties of the atmosphere. The effects include scintillations, path length fluctuations, spreading of a laser beam, deflection of the beam, and depolarization. The physical properties that may be measured include the average temperature along the path, the vertical temperature gradient, and the distribution along the path of the strength of turbulence and the transverse wind velocity. Line-of-sight laser beam methods are clearly effective in measuring the average properties, but less effective in measuring distributions along the path. Fundamental limitations to the resolution are pointed out and experiments are recommended to investigate the practicality of the methods.

Confounding factors in the use of the zero-heat-flow method for non-invasive muscle temperature measurement.

PubMed

Brajkovic, Dragan; Ducharme, Michel B

2005-07-01

This study evaluated a zero-heat-flow (ZHF), non-invasive temperature probe for in- vivo measurement of resting muscle temperature for up to 2 cm below the skin surface. The ZHF probe works by preventing heat loss from the tissue below the probe by actively heating the tissue until no temperature gradient exists across the probe. The skin temperature under the probe is then used as an indicator of the muscle temperature below. Eight subjects sat for 130 min during exposure to 28 degrees C air. Vastus lateralis (lateral thigh) muscle temperature was measured non-invasively using a ZHF probe which covered an invasive multicouple probe (which measured tissue temperature 0.5 cm, 1 cm, 1.5 cm, and 2 cm below the skin) located 15 cm superior to the patella (T (covered)). T (covered) was evaluated against an uncovered control multicouple probe located 20 cm superior to the patella (T (uncovered)). Rectal temperature and lateral thigh skin temperature were also measured. Mean T (uncovered) (based on average temperatures at the 0.5 cm, 1 cm, 1.5 cm, and 2 cm depths) and Mean T (covered) were similar from time 0 min to 60 min. However, when the ZHF was turned on at 70 min, Mean T (covered) increased by 2.11 +/- 0.20 degrees C by 130 min, while T (uncovered) remained stable. The ZHF probe temperature was similar to T (covered) at 1 cm and after time 85 min, significantly higher than T (covered) at the 0.5 cm, 1.5 cm, and 2 cm depths; however from a physiological standpoint, the temperatures between the different depths and the ZHF probe could be considered uniform (< or =0.2 degrees C separation). Rectal and thigh skin temperatures were stable at 36.99 +/- 0.08 degrees C and 32.82 +/- 0.23 degrees C, respectively. In conclusion, the non-invasive ZHF probe temperature was similar to the T (covered) temperatures directly measured up to 2 cm beneath the surface of the thigh, but all T (covered) temperatures were not representative of the true muscle temperature up to 2 cm below the skin because the ZHF probe heated the muscle by 2.11 +/- 0.20 degrees C during its operation.

Traversing probe system

DOEpatents

Mashburn, Douglas N.; Stevens, Richard H.; Woodall, Harold C.

1977-01-01

This invention comprises a rotatable annular probe-positioner which carries at least one radially disposed sensing probe, such as a Pitot tube having a right-angled tip. The positioner can be coaxially and rotatably mounted within a compressor casing or the like and then actuated to orient the sensing probe as required to make measurements at selected stations in the annulus between the positioner and compressor casing. The positioner can be actuated to (a) selectively move the probe along its own axis, (b) adjust the yaw angle of the right-angled probe tip, and (c) revolve the probe about the axis common to the positioner and casing. A cam plate engages a cam-follower portion of the probe and normally rotates with the positioner. The positioner includes a first-motor-driven ring gear which effects slidable movement of the probe by rotating the positioner at a time when an external pneumatic cylinder is actuated to engage the cam plate and hold it stationary. When the pneumatic cylinder is not actuated, this ring gear can be driven to revolve the positioner and thus the probe to a desired circumferential location about the above-mentioned common axis. A second motor-driven ring gear included in the positioner can be driven to rotate the probe about its axis, thus adjusting the yaw angle of the probe tip. The positioner can be used in highly corrosive atmosphere, such as gaseous uranium hexafluoride.

Incidences of esophageal injury during esophageal temperature monitoring: a comparative study of a multi-thermocouple temperature probe and a deflectable temperature probe in atrial fibrillation ablation.

PubMed

Kuwahara, Taishi; Takahashi, Atsushi; Takahashi, Yoshihide; Okubo, Kenji; Takagi, Katsumasa; Fujino, Tadashi; Kusa, Shigeki; Takigawa, Masateru; Watari, Yuji; Yamao, Kazuya; Nakashima, Emiko; Kawaguchi, Naohiko; Hikita, Hiroyuki; Sato, Akira; Aonuma, Kazutaka

2014-04-01

The study aim was to compare the incidence of esophageal injuries between different temperature probes in the monitoring of esophageal temperature during atrial fibrillation (AF) ablation. One hundred patients with drug-resistant AF were prospectively and randomly assigned into two groups according to the esophageal temperature probe used: the multi-thermocouple probe group (n = 50) and the deflectable temperature probe group (n = 50). Extensive pulmonary vein (PV) isolation was performed with a 3.5-mm open irrigated tip ablation catheter by using a radiofrequency (RF) power of 25-30 W. In both groups, the esophageal temperature thermocouple was placed on the area of the esophagus adjacent to the ablation site. When the esophageal temperature reached 42 °C, the RF energy delivery was stopped. Esophageal endoscopy was performed 1 day after the catheter ablation. No differences existed between the two groups in terms of clinical background and various parameters related to the catheter ablation, including RF delivery time and number of RF deliveries at an esophageal temperature of >42 °C. Esophageal lesions, such as esophagitis and esophageal ulcers, occurred in 10/50 (20 %) and 15/50 (30 %) patients in the multi-thermocouple and deflectable temperature probe groups, respectively (P = 0.25). Most lesions were mild to moderate injuries, and all were cured using conservative treatment. The incidence of esophageal injury was almost equal between the multi-thermocouple temperature probe and the deflectable temperature probe during esophageal temperature monitoring. Most of the esophageal lesions that developed during esophageal temperature monitoring were mild to moderate and reversible.

Optimization and thermal ASE noise characterization of an all-fibre Sagnac interferometer via LAN for sensing applications

NASA Astrophysics Data System (ADS)

Sierra-Calderon, A.; Rodriguez-Novelo, J. C.; Gamez-Aviles, E.; May-Alarcon, M.; Toral-Cruz, H.; Alvarez-Chavez, J. A.

2016-09-01

The spectral noise characteristic and relative intensity noise of an all fibre Sagnac interferometer system consisting of a 980nm pump source at 130mW maximum output power, a 980/1550nm wavelength division multiplexer, a 10m-piece of Erbium-doped fibre, a fibre Bragg grating (FBG) centered at 1.548um, an optical circulator at 1550nm and a 50/50 fibre coupler, were measured with an optical spectrum analyzer (OSA) for fine tuning for a range of temperature between 5 and 180 degrees Celsius in step of 1 degree Celsius. At the probing end, a high-bi piece of fibre and a Peltier were employed for temperature variation of the system. Spectral and temperature response of the noise reduction due to temperature variation was performed remotely using and Arduino micro-controller and a DS18B20 digital sensor, into a local area network. Full optical and thermal characterization of the system will be included in the presentation.

Evaluation of water cooled supersonic temperature and pressure probes for application to 2000 F flows

NASA Technical Reports Server (NTRS)

Lagen, Nicholas T.; Seiner, John M.

1990-01-01

The development of water cooled supersonic probes used to study high temperature jet plumes is addressed. These probes are: total pressure, static pressure, and total temperature. The motivation for these experiments is the determination of high temperature supersonic jet mean flow properties. A 3.54 inch exit diameter water cooled nozzle was used in the tests. It is designed for exit Mach 2 at 2000 F exit total temperature. Tests were conducted using water cooled probes capable of operating in Mach 2 flow, up to 2000 F total temperature. Of the two designs tested, an annular cooling method was chosen as superior. Data at the jet exit planes, and along the jet centerline, were obtained for total temperatures of 900 F, 1500 F, and 2000 F, for each of the probes. The data obtained from the total and static pressure probes are consistent with prior low temperature results. However, the data obtained from the total temperature probe was affected by the water coolant. The total temperature probe was tested up to 2000 F with, and without, the cooling system turned on to better understand the heat transfer process at the thermocouple bead. The rate of heat transfer across the thermocouple bead was greater when the coolant was turned on than when the coolant was turned off. This accounted for the lower temperature measurement by the cooled probe. The velocity and Mach number at the exit plane and centerline locations were determined from the Rayleigh-Pitot tube formula.

Fiber optic sensors IV; Proceedings of the Third European Congress on Optics, The Hague, Netherlands, Mar. 13, 14, 1990

NASA Technical Reports Server (NTRS)

Kersten, Ralf T. (Editor)

1990-01-01

Recent advances in fiber-optic sensor (FOS) technology are examined in reviews and reports. Sections are devoted to components for FOSs, special fibers for FOSs, interferometry, FOS applications, and sensing principles and influence. Particular attention is given to solder glass sealing technology for FOS packaging, the design of optical-fiber current sensors, pressure and temperature effects on beat length in highly birefringent optical fibers, a pressure FOS based on vibrating-quartz-crystal technology, remote sensing of flammable gases using a fluoride-fiber evanescent probe, a displacement sensor with electronically scanned white-light interferometer, the use of multimode laser diodes in low-coherence coupled-cavity interferometry, electronic speckle interferometry compensated for environmentally induced phase noise, a dual-resolution noncontact vibration and displacement sensor based on a two-wavelength source, and fiber optics in composite materials.

Pressure sensing of Fabry-Perot interferometer with a microchannel demodulated by a FBG

NASA Astrophysics Data System (ADS)

Yu, Yongqin; Chen, Xue; Huang, Quandong; Du, Chenlin; Ruan, Shuangchen

2015-07-01

A novel and compact fiber-probe pressure sensor was demonstrated based on micro Fabry-Perot interferometer (FPI). The device was fabricated by splicing both ends of a short section simplified hollow-core photonic crystal fiber (SHCPCF) with single mode fibers (SMFs), and then a micro channel was drilled by femtosecond laser micromachining in the SHC-PCF to significantly enhance the pressure sensitivity. The pressure sensing characteristics based on micro-FPI have been investigated by measuring the signals through the demodulation of phase since the external signal imposing on the interferometer will induce the phase change of interference signal. Then a FBG was cascaded to demodulate the signal. A micro FPI demonstrates a maximum pressure sensitivity of 32 dB/MPa, while a low temperature cross-sensitivity of 0.27 KPa/°C. Hence it may have potential for pressure applications in harsh environment.

Light-induced pyroelectric effect as an effective approach for ultrafast ultraviolet nanosensing

NASA Astrophysics Data System (ADS)

Wang, Zhaona; Yu, Ruomeng; Pan, Caofeng; Li, Zhaoling; Yang, Jin; Yi, Fang; Wang, Zhong Lin

2015-09-01

Zinc oxide is potentially a useful material for ultraviolet detectors; however, a relatively long response time hinders practical implementation. Here by designing and fabricating a self-powered ZnO/perovskite-heterostructured ultraviolet photodetector, the pyroelectric effect, induced in wurtzite ZnO nanowires on ultraviolet illumination, has been utilized as an effective approach for high-performance photon sensing. The response time is improved from 5.4 s to 53 μs at the rising edge, and 8.9 s to 63 μs at the falling edge, with an enhancement of five orders in magnitudes. The specific detectivity and the responsivity are both enhanced by 322%. This work provides a novel design to achieve ultrafast ultraviolet sensing at room temperature via light-self-induced pyroelectric effect. The newly designed ultrafast self-powered ultraviolet nanosensors may find promising applications in ultrafast optics, nonlinear optics, optothermal detections, computational memories and biocompatible optoelectronic probes.

Fast and accurate determination of the detergent efficiency by optical fiber sensors

NASA Astrophysics Data System (ADS)

Patitsa, Maria; Pfeiffer, Helge; Wevers, Martine

2011-06-01

An optical fiber sensor was developed to control the cleaning efficiency of surfactants. Prior to the measurements, the sensing part of the probe is covered with a uniform standardized soil layer (lipid multilayer), and a gold mirror is deposited at the end of the optical fiber. For the lipid multilayer deposition on the fiber, Langmuir-Blodgett technique was used and the progress of deposition was followed online by ultraviolet spectroscopy. The invention provides a miniaturized Surface Plasmon Resonance dip-sensor for automated on-line testing that can replace the cost and time consuming existing methods and develop a breakthrough in detergent testing in combining optical sensing, surface chemistry and automated data acquisition. The sensor is to be used to evaluate detergency of different cleaning products and also indicate how formulation, concentration, lipid nature and temperature affect the cleaning behavior of a surfactant.

High-Temperature Thermometer Using Cr-Doped GdAlO3 Broadband Luminescence

NASA Technical Reports Server (NTRS)

Eldridge, Jeffrey; Chambers, Matthew

2011-01-01

A new concept has been developed for a high-temperature luminescence-based optical thermometer that both shows the desired temperature sensitivity in the upper temperature range of present state-of-the-art luminescence thermometers (above 1,300 C), while maintaining substantial stronger luminescence signal intensity that will allow these optical thermometers to operate in the presence of the high thermal background radiation typical of industrial applications. This objective is attained by using a Cr-doped GdAlO3 (Cr:GdAlO3) sensor with an orthorhombic perovskite structure, resulting in broadband luminescence that remains strong at high temperature due to the favorable electron energy level spacing of Cr:GdAlO3. The Cr:GdAlO3 temperature (and pressure) sensor can be incorporated into, or applied onto, a component s surface when a non-contact surface temperature measurement is desired, or alternatively, the temperature sensor can be attached to the end of a fiber-optic probe that can then be positioned at the location where the temperature measurement is desired. In the case of the fiber-optic probe, both the pulsed excitation and the luminescence emission travel through the fiber-optic light guide. In either case, a pulsed light source provides excitation of the luminescence, and the broadband luminescence emission is collected. Real-time temperature measurements are obtain ed using a least-squares fitting algorithm that determines the luminescence decay time, which has a known temperature dependence established by calibration. Due to the broad absorption and emission bands for Cr:GdAlO3, there is considerable flexibility in the choice of excitation wavelength and emission wavelength detection bands. The strategic choice of the GdAlO3 host is based on its high crystal field, phase stability, and distorted symmetry at the Cr3+ occupation sites. The use of the broadband emission for temperature sensing at high temperatures is a key feature of the invention and is novel since broadband luminescence emission normally shows severe thermal quenching. The tightly bound AlO6 octahedra in GdAlO3 results in a larger energy barrier to nonradiative decays than in other materials and therefore makes using broadband emission for temperature sensing possible at high temperatures. This approach results in a substantial increase in temperature capability. For example, the most commonly used Cr-doped crystal used for luminescence-based temperature measurements, ruby, has only been demonstrated up to 600 C, whereas the Cr:GdAlO3 optical thermometer under development has already been shown to exhibit useful luminescence up to 1,300 C. Because GdAlO3 is non-reactive and is stable in harsh, high-temperature environments, sensors composed of Cr:GdAlO3 will be very well suited for remote high-temperature measurements in engine or industrial environments where its intense high-temperature luminescence will stand out above significant thermal radiation background levels.

NIR fluorescence lifetime sensing through a multimode fiber for intravascular molecular probing

NASA Astrophysics Data System (ADS)

Ingelberts, H.; Hernot, S.; Debie, P.; Lahoutte, T.; Kuijk, M.

2016-04-01

Coronary artery disease (CAD) contributes to millions of deaths each year. The identification of vulnerable plaques is essential to the diagnosis of CAD but is challenging. Molecular probes can improve the detection of these plaques using intravascular imaging methods. Fluorescence lifetime sensing is a safe and robust method to image these molecular probes. We present two variations of an optical system for intravascular near-infrared (NIR) fluorescence lifetime sensing through a multimode fiber. Both systems are built around a recently developed fast and efficient CMOS detector, the current-assisted photonic sampler (CAPS) that is optimized for sub-nanosecond NIR fluorescence lifetime sensing. One system mimics the optical setup of an epifluorescence microscope while the other uses a practical fiber optic coupler to separate fluorescence excitation and emission. We test both systems by measuring the lifetime of several NIR dyes in DMSO solutions and we show that these systems are capable of detecting lifetimes of solutions with concentrations down to 370 nM and this with short acquisition times. These results are compared with time-correlated single photon counting (TCSPC) measurements for reference.

Multifunctional PHPMA-Derived Polymer for Ratiometric pH Sensing, Fluorescence Imaging, and Magnetic Resonance Imaging.

PubMed

Su, Fengyu; Agarwal, Shubhangi; Pan, Tingting; Qiao, Yuan; Zhang, Liqiang; Shi, Zhengwei; Kong, Xiangxing; Day, Kevin; Chen, Meiwan; Meldrum, Deirdre; Kodibagkar, Vikram D; Tian, Yanqing

2018-01-17

In this paper, we report synthesis and characterization of a novel multimodality (MRI/fluorescence) probe for pH sensing and imaging. A multifunctional polymer was derived from poly(N-(2-hydroxypropyl)methacrylamide) (PHPMA) and integrated with a naphthalimide-based-ratiometric fluorescence probe and a gadolinium-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid complex (Gd-DOTA complex). The polymer was characterized using UV-vis absorption spectrophotometry, fluorescence spectrofluorophotometry, magnetic resonance imaging (MRI), and confocal microscopy for optical and MRI-based pH sensing and cellular imaging. In vitro labeling of macrophage J774 and esophageal CP-A cell lines shows the polymer's ability to be internalized in the cells. The transverse relaxation time (T 2 ) of the polymer was observed to be pH-dependent, whereas the spin-lattice relaxation time (T 1 ) was not. The pH probe in the polymer shows a strong fluorescence-based ratiometric pH response with emission window changes, exhibiting blue emission under acidic conditions and green emission under basic conditions, respectively. This study provides new materials with multimodalities for pH sensing and imaging.

Polydiacetylene-enclosed near-infrared fluorescent semiconducting polymer dots for bioimaging and sensing.

PubMed

Wu, Pei-Jing; Kuo, Shih-Yu; Huang, Ya-Chi; Chen, Chuan-Pin; Chan, Yang-Hsiang

2014-05-20

Semiconducting polymer dots (P-dots) recently have emerged as a new type of ultrabright fluorescent probe with promising applications in biological imaging and detection. With the increasing desire for near-infrared (NIR) fluorescing probes for in vivo biological measurements, the currently available NIR-emitting P-dots are very limited and the leaching of the encapsulated dyes/polymers has usually been a concern. To address this challenge, we first embedded the NIR dyes into the matrix of poly[(9,9-dioctylfluorene)-co-2,1,3-benzothiadiazole-co-4,7-di(thiophen-2-yl)-2,1,3-benzothiadiazole] (PF-BT-DBT) polymer and then enclosed the doped P-dots with polydiacetylenes (PDAs) to avoid potential leakage of the entrapped NIR dyes from the P-dot matrix. These PDA-enclosed NIR-emitting P-dots not only emitted much stronger NIR fluorescence than conventional organic molecules but also exhibited enhanced photostability over CdTe quantum dots, free NIR dyes, and gold nanoclusters. We next conjugated biomolecules onto the surface of the resulting P-dots and demonstrated their capability for specific cellular labeling without any noticeable nonspecific binding. To employ this new class of material as a facile sensing platform, an easy-to-prepare test paper, obtained by soaking the paper into the PDA-enclosed NIR-emitting P-dot solution, was used to sense external stimuli such as ions, temperature, or pH, depending on the surface functionalization of PDAs. We believe these PDA-coated NIR-fluorescing P-dots will be very useful in a variety of bioimaging and analytical applications.

Probing the Hydrogen Enhanced Near-Field Emission of ITO without a Vacuum-Gap

DOE Office of Scientific and Technical Information (OSTI.GOV)

Poole, Jacob L.; Yu, Yang; Ohodnicki, Paul R.

In-situ monitoring of the multi-component gas streams in high temperature energy conversion devices offer the promises to higher efficiency via improved understanding of the chemical environments during device operation. While conventional resistive based metal oxide semiconductor gas sensors suffer from strong cross-sensitivity, optical sensing approaches offer intrinsic advantages to achieve gas selectivity based on wavelength specific interactions. This manuscript describes a novel method to achieve multicomponent gas sensing during gas exposure of H2, CO2, CH4and CO in humid high temperature environments. A single sensor element comprised of a perovskite La0.3Sr0.7TiO3(LSTO) oxide thin film layer coated on silica optical fiber wasmore » used. The sensing responses consisted of two wavelength-specific near infrared (NIR) mechanisms, namely broadband absorption associated with the metal oxide layer, and wavelength localized thermal emission responses associated with the hydroxyl defects within the silica fiber. Principal component analysis (PCA) was applied to couple the two mechanisms to achieve selective gas identification. Successful discrimination of H2and CO2on a single fiber sensor was achieved, where the results are both stable and reversible. This design demonstrates that by coupling multiple optical mechanisms on a single oxide coated fiber sensor, simple platforms can also achieve multi-component sensing functionality without the added complexity of a sensor array. Thus, it suggests a new approach to construct simple, robust and functional sensor designs capable of gas discrimination and quantification in multi-component gas streams.« less

Parameter Estimation of the Thermal Network Model of a Machine Tool Spindle by Self-made Bluetooth Temperature Sensor Module

PubMed Central

Lo, Yuan-Chieh; Hu, Yuh-Chung; Chang, Pei-Zen

2018-01-01

Thermal characteristic analysis is essential for machine tool spindles because sudden failures may occur due to unexpected thermal issue. This article presents a lumped-parameter Thermal Network Model (TNM) and its parameter estimation scheme, including hardware and software, in order to characterize both the steady-state and transient thermal behavior of machine tool spindles. For the hardware, the authors develop a Bluetooth Temperature Sensor Module (BTSM) which accompanying with three types of temperature-sensing probes (magnetic, screw, and probe). Its specification, through experimental test, achieves to the precision ±(0.1 + 0.0029|t|) °C, resolution 0.00489 °C, power consumption 7 mW, and size Ø40 mm × 27 mm. For the software, the heat transfer characteristics of the machine tool spindle correlative to rotating speed are derived based on the theory of heat transfer and empirical formula. The predictive TNM of spindles was developed by grey-box estimation and experimental results. Even under such complicated operating conditions as various speeds and different initial conditions, the experiments validate that the present modeling methodology provides a robust and reliable tool for the temperature prediction with normalized mean square error of 99.5% agreement, and the present approach is transferable to the other spindles with a similar structure. For realizing the edge computing in smart manufacturing, a reduced-order TNM is constructed by Model Order Reduction (MOR) technique and implemented into the real-time embedded system. PMID:29473877

Parameter Estimation of the Thermal Network Model of a Machine Tool Spindle by Self-made Bluetooth Temperature Sensor Module.

PubMed

Lo, Yuan-Chieh; Hu, Yuh-Chung; Chang, Pei-Zen

2018-02-23

Thermal characteristic analysis is essential for machine tool spindles because sudden failures may occur due to unexpected thermal issue. This article presents a lumped-parameter Thermal Network Model (TNM) and its parameter estimation scheme, including hardware and software, in order to characterize both the steady-state and transient thermal behavior of machine tool spindles. For the hardware, the authors develop a Bluetooth Temperature Sensor Module (BTSM) which accompanying with three types of temperature-sensing probes (magnetic, screw, and probe). Its specification, through experimental test, achieves to the precision ±(0.1 + 0.0029|t|) °C, resolution 0.00489 °C, power consumption 7 mW, and size Ø40 mm × 27 mm. For the software, the heat transfer characteristics of the machine tool spindle correlative to rotating speed are derived based on the theory of heat transfer and empirical formula. The predictive TNM of spindles was developed by grey-box estimation and experimental results. Even under such complicated operating conditions as various speeds and different initial conditions, the experiments validate that the present modeling methodology provides a robust and reliable tool for the temperature prediction with normalized mean square error of 99.5% agreement, and the present approach is transferable to the other spindles with a similar structure. For realizing the edge computing in smart manufacturing, a reduced-order TNM is constructed by Model Order Reduction (MOR) technique and implemented into the real-time embedded system.

Carbon granule probe microphone for leak detection. [recovery boilers

NASA Technical Reports Server (NTRS)

Parthasarathy, S. P. (Inventor)

1985-01-01

A microphone which is not subject to corrosion is provided by employing carbon granules to sense sound waves. The granules are packed into a ceramic tube and no diaphragm is used. A pair of electrodes is located in the tube adjacent the carbon granules and are coupled to a sensing circuit. Sound waves cause pressure changes on the carbon granules which results in a change in resistance in the electrical path between the electrodes. This change in resistance is detected by the sensing circuit. The microphone is suitable for use as a leak detection probe in recovery boilers, where it provides reliable operation without corrosion problems associated with conventional microphones.

RF current sensor

DOEpatents

Moore, James A.; Sparks, Dennis O.

1998-11-10

An RF sensor having a novel current sensing probe and a voltage sensing probe to measure voltage and current. The current sensor is disposed in a transmission line to link all of the flux generated by the flowing current in order to obtain an accurate measurement. The voltage sensor is a flat plate which operates as a capacitive plate to sense voltage on a center conductor of the transmission line, in which the measured voltage is obtained across a resistance leg of a R-C differentiator circuit formed by the characteristic impedance of a connecting transmission line and a capacitance of the plate, which is positioned proximal to the center conductor.

Biomedical and sensing applications of a multi-mode biodegradable phosphate-based optical fiber

NASA Astrophysics Data System (ADS)

Podrazky, Ondřej; Peterka, Pavel; Vytykáčová, SoÅa.; Proboštová, Jana; Kuneš, Martin; Lyutakov, Oleksiy; Ceci-Ginistrelli, Edoardo; Pugliese, Diego; Boetti, Nadia G.; Janner, Davide; Milanese, Daniel

2018-02-01

We report on the employment of a biodegradable phosphate-based optical fiber as a pH sensing probe in physiological environment. The phosphate-based optical fiber preform was fabricated by the rod-in-tube technique. The fiber biodegradability was first tested in-vitro and then its biodegradability and toxicity were tested in-vivo. Optical probes for pH sensing were prepared by the immobilization of a fluorescent dye on the fiber tip by a sol-gel method. The fluorescence response of the pH-sensor was measured as a ratio of the emission intensities at the excitation wavelengths of 405 and 450 nm.

Difference in thermodynamics between two types of esophageal temperature probes: Insights from an experimental study.

PubMed

Gianni, Carola; Atoui, Moustapha; Mohanty, Sanghamitra; Trivedi, Chintan; Bai, Rong; Al-Ahmad, Amin; Burkhardt, J David; Gallinghouse, G Joseph; Hranitzky, Patrick M; Horton, Rodney P; Sanchez, Javier E; Di Biase, Luigi; Lakkireddy, Dhanunjaya R; Natale, Andrea

2016-11-01

Luminal esophageal temperature monitoring is performed with a variety of temperature probes, but little is known about the relationship between the structure of a given probe and its thermodynamic characteristics. The purpose of this study was to evaluate the difference in thermodynamics between a 9Fr standard esophageal probe and an 18Fr esophageal stethoscope. In the experimental setting, each probe was submerged in a constant temperature water bath maintained at 42°C; in the patient setting, we monitored the temperature with both probes at the same time. The time constant of the stethoscope was higher than that of the probe (33.5 vs 8.3 s). Compared to the probe, the mean temperature measured by the stethoscope at 10 seconds was significantly lower (22.5°C ± 0.4°C vs 33.5°C ± 0.3°C, P<.0001), whereas the time to reach the peak temperature was significantly longer (132.6 ± 5.9 s vs 38.8 ± 1.0 s, P<.0001). Even in the ablation cases we observed that when the esophageal probe reached a peak temperature of 39.6°C ± 0.3°C, the esophageal stethoscope still displayed a temperature of 37.3°C ± 0.2°C (a mean of 2.39°C ± 0.3°C lower, P<.0001), showing a <0.5°C increase in temperature half of the times. The 18Fr esophageal stethoscope has a significantly slower time response compared to the 9Fr esophageal probe. In the clinical setting, this might result in a considerable underestimation of the luminal esophageal temperature with potentially fatal consequences. Copyright © 2016 Heart Rhythm Society. Published by Elsevier Inc. All rights reserved.

Electron-beam-induced topographical, chemical, and structural patterning of amorphous titanium oxide films.

PubMed

Kern, P; Müller, Y; Patscheider, J; Michler, J

2006-11-30

Electrolytically deposited amorphous TiO2 films on steel are remarkably sensitive to electron beam (e-beam) irradiation at moderate energies at 20 keV, resulting in controlled local oxide reduction and crystallization, opening the possibility for local topographical, chemical, and structural modifications within a biocompatible, amorphous, and semiconducting matrix. The sensitivity is shown to vary significantly with the annealing temperature of as-deposited films. Well-defined irradiation conditions in terms of probe current IP (5 microA) and beam size were achieved with an electron probe microanalyzer. As shown by atomic force and optical microscopy, micro-Raman spectroscopy, wavelength-dispersive X-ray (WDX), and Auger analyses, e-beam exposure below 1 Acm-2 immediately leads to electron-stimulated oxygen desorption, resulting in a well-defined volume loss primarily limited to the irradiated zone under the electron probe and in a blue color shift in this zone because of the presence of Ti2O3. Irradiation at 5 Acm(-2) (IP = 5 microA) results in local crystallization into anatase phase within 1 s of exposure and in reduction to TiO after an extended exposure of 60 s. Further reduction to the metallic state could be observed after 60 s of exposure at approximately 160 Acm(-2). The local reduction could be qualitatively sensed with WDX analysis and Auger line scans. An estimation of the film temperature in the beam center indicates that crystallization occurs at less than 150 degrees C, well below the atmospheric crystallization temperature of the present films. The high e-beam sensitivity in combination with the well-defined volume loss from oxygen desorption allows for precise electron lithographic topographical patterning of the present oxides. Irradiation effects leading to the observed reduction and crystallization phenomena under moderate electron energies are discussed.

Real-time temperature monitoring during radiofrequency treatments on ex-vivo animal model by fiber Bragg grating sensors

NASA Astrophysics Data System (ADS)

Palumbo, Giovanna; Tosi, Daniele; Schena, Emiliano; Massaroni, Carlo; Ippolito, Juliet; Verze, Paolo; Carlomagno, Nicola; Tammaro, Vincenzo; Iadicicco, Agostino; Campopiano, Stefania

2017-05-01

Fiber Bragg Grating (FBG) sensors applied to bio-medical procedures such as surgery and rehabilitation are a valid alternative to traditional sensing techniques due to their unique characteristics. Herein we propose the use of FBG sensor arrays for accurate real-time temperature measurements during multi-step RadioFrequency Ablation (RFA) based thermal tumor treatment. Real-time temperature monitoring in the RF-applied region represents a valid feedback for the success of the thermo-ablation procedure. In order to create a thermal multi-point map around the tumor area to be treated, a proper sensing configuration was developed. In particular, the RF probe of a commercial medical instrumentation, has been equipped with properly packaged FBGs sensors. Moreover, in order to discriminate the treatment areas to be ablated as precisely as possible, a second array 3.5 cm long, made by several FBGs was used. The results of the temperature measurements during the RFA experiments conducted on ex-vivo animal liver and kidney tissues are presented herein. The proposed FBGs based solution has proven to be capable of distinguish different and consecutive discharges and for each of them, to measure the temperature profile with a resolution of 0.1 °C and a minimum spatial resolution of 5mm. Based upon our experiments, it is possible to confirm that the temperature decreases with distance from a RF peak ablation, in accordance with RF theory. The proposed solution promises to be very useful for the surgeon because a real-time temperature feedback allows for the adaptation of RFA parameters during surgery and better delineates the area under treatment.

Continuous sensing of tumor-targeted molecular probes with a vertical cavity surface emitting laser-based biosensor

NASA Astrophysics Data System (ADS)

Parashurama, Natesh; O'Sullivan, Thomas D.; De La Zerda, Adam; El Kalassi, Pascale; Cho, Seongjae; Liu, Hongguang; Teed, Robert; Levy, Hart; Rosenberg, Jarrett; Cheng, Zhen; Levi, Ofer; Harris, James S.; Gambhir, Sanjiv S.

2012-11-01

Molecular optical imaging is a widespread technique for interrogating molecular events in living subjects. However, current approaches preclude long-term, continuous measurements in awake, mobile subjects, a strategy crucial in several medical conditions. Consequently, we designed a novel, lightweight miniature biosensor for in vivo continuous optical sensing. The biosensor contains an enclosed vertical-cavity surface-emitting semiconductor laser and an adjacent pair of near-infrared optically filtered detectors. We employed two sensors (dual sensing) to simultaneously interrogate normal and diseased tumor sites. Having established the sensors are precise with phantom and in vivo studies, we performed dual, continuous sensing in tumor (human glioblastoma cells) bearing mice using the targeted molecular probe cRGD-Cy5.5, which targets αVβ3 cell surface integrins in both tumor neovasculature and tumor. The sensors capture the dynamic time-activity curve of the targeted molecular probe. The average tumor to background ratio after signal calibration for cRGD-Cy5.5 injection is approximately 2.43±0.95 at 1 h and 3.64±1.38 at 2 h (N=5 mice), consistent with data obtained with a cooled charge coupled device camera. We conclude that our novel, portable, precise biosensor can be used to evaluate both kinetics and steady state levels of molecular probes in various disease applications.

Continuous sensing of tumor-targeted molecular probes with a vertical cavity surface emitting laser-based biosensor

PubMed Central

Parashurama, Natesh; O’Sullivan, Thomas D.; De La Zerda, Adam; El Kalassi, Pascale; Cho, Seongjae; Liu, Hongguang; Teed, Robert; Levy, Hart; Rosenberg, Jarrett; Cheng, Zhen; Levi, Ofer; Harris, James S.

2012-01-01

Abstract. Molecular optical imaging is a widespread technique for interrogating molecular events in living subjects. However, current approaches preclude long-term, continuous measurements in awake, mobile subjects, a strategy crucial in several medical conditions. Consequently, we designed a novel, lightweight miniature biosensor for in vivo continuous optical sensing. The biosensor contains an enclosed vertical-cavity surface-emitting semiconductor laser and an adjacent pair of near-infrared optically filtered detectors. We employed two sensors (dual sensing) to simultaneously interrogate normal and diseased tumor sites. Having established the sensors are precise with phantom and in vivo studies, we performed dual, continuous sensing in tumor (human glioblastoma cells) bearing mice using the targeted molecular probe cRGD-Cy5.5, which targets αVβ3 cell surface integrins in both tumor neovasculature and tumor. The sensors capture the dynamic time-activity curve of the targeted molecular probe. The average tumor to background ratio after signal calibration for cRGD-Cy5.5 injection is approximately 2.43±0.95 at 1 h and 3.64±1.38 at 2 h (N=5 mice), consistent with data obtained with a cooled charge coupled device camera. We conclude that our novel, portable, precise biosensor can be used to evaluate both kinetics and steady state levels of molecular probes in various disease applications. PMID:23123976

A Wireless Passive Sensing System for Displacement/Strain Measurement in Reinforced Concrete Members

PubMed Central

Ozbey, Burak; Erturk, Vakur B.; Demir, Hilmi Volkan; Altintas, Ayhan; Kurc, Ozgur

2016-01-01

In this study, we show a wireless passive sensing system embedded in a reinforced concrete member successfully being employed for the measurement of relative displacement and strain in a simply supported beam experiment. The system utilizes electromagnetic coupling between the transceiver antenna located outside the beam, and the sensing probes placed on the reinforcing bar (rebar) surface inside the beam. The probes were designed in the form of a nested split-ring resonator, a metamaterial-based structure chosen for its compact size and high sensitivity/resolution, which is at µm/microstrains level. Experiments were performed in both the elastic and plastic deformation cases of steel rebars, and the sensing system was demonstrated to acquire telemetric data in both cases. The wireless measurement results from multiple probes are compared with the data obtained from the strain gages, and an excellent agreement is observed. A discrete time measurement where the system records data at different force levels is also shown. Practical issues regarding the placement of the sensors and accurate recording of data are discussed. The proposed sensing technology is demonstrated to be a good candidate for wireless structural health monitoring (SHM) of reinforced concrete members by its high sensitivity and wide dynamic range. PMID:27070615

Reflection-mode micro-spherical fiber-optic probes for in vitro real-time and single-cell level pH sensing.

PubMed

Yang, Qingbo; Wang, Hanzheng; Lan, Xinwei; Cheng, Baokai; Chen, Sisi; Shi, Honglan; Xiao, Hai; Ma, Yinfa

2015-02-01

pH sensing at the single-cell level without negatively affecting living cells is very important but still a remaining issue in the biomedical studies. A 70 μm reflection-mode fiber-optic micro-pH sensor was designed and fabricated by dip-coating thin layer of organically modified aerogel onto a tapered spherical probe head. A pH sensitive fluorescent dye 2', 7'-Bis (2-carbonylethyl)-5(6)-carboxyfluorescein (BCECF) was employed and covalently bonded within the aerogel networks. By tuning the alkoxide mixing ratio and adjusting hexamethyldisilazane (HMDS) priming procedure, the sensor can be optimized to have high stability and pH sensing ability. The in vitro real-time sensing capability was then demonstrated in a simple spectroscopic way, and showed linear measurement responses with a pH resolution up to an average of 0.049 pH unit within a narrow, but biological meaningful pH range of 6.12-7.81. Its novel characterizations of high spatial resolution, reflection mode operation, fast response and high stability, great linear response within biological meaningful pH range and high pH resolutions, make this novel pH probe a very cost-effective tool for chemical/biological sensing, especially within the single cell level research field.

Reflection-mode micro-spherical fiber-optic probes for in vitro real-time and single-cell level pH sensing

PubMed Central

Yang, Qingbo; Wang, Hanzheng; Lan, Xinwei; Cheng, Baokai; Chen, Sisi; Shi, Honglan; Xiao, Hai; Ma, Yinfa

2014-01-01

pH sensing at the single-cell level without negatively affecting living cells is very important but still a remaining issue in the biomedical studies. A 70 μm reflection-mode fiber-optic micro-pH sensor was designed and fabricated by dip-coating thin layer of organically modified aerogel onto a tapered spherical probe head. A pH sensitive fluorescent dye 2′, 7′-Bis (2-carbonylethyl)-5(6)-carboxyfluorescein (BCECF) was employed and covalently bonded within the aerogel networks. By tuning the alkoxide mixing ratio and adjusting hexamethyldisilazane (HMDS) priming procedure, the sensor can be optimized to have high stability and pH sensing ability. The in vitro real-time sensing capability was then demonstrated in a simple spectroscopic way, and showed linear measurement responses with a pH resolution up to an average of 0.049 pH unit within a narrow, but biological meaningful pH range of 6.12–7.81. Its novel characterizations of high spatial resolution, reflection mode operation, fast response and high stability, great linear response within biological meaningful pH range and high pH resolutions, make this novel pH probe a very cost-effective tool for chemical/biological sensing, especially within the single cell level research field. PMID:25530670

Flame exposure time on Langmuir probe degradation, ion density, and thermionic emission for flame temperature.

PubMed

Doyle, S J; Salvador, P R; Xu, K G

2017-11-01

The paper examines the effect of exposure time of Langmuir probes in an atmospheric premixed methane-air flame. The effects of probe size and material composition on current measurements were investigated, with molybdenum and tungsten probe tips ranging in diameter from 0.0508 to 0.1651 mm. Repeated prolonged exposures to the flame, with five runs of 60 s, resulted in gradual probe degradations (-6% to -62% area loss) which affected the measurements. Due to long flame exposures, two ion saturation currents were observed, resulting in significantly different ion densities ranging from 1.16 × 10 16 to 2.71 × 10 19 m -3 . The difference between the saturation currents is caused by thermionic emissions from the probe tip. As thermionic emission is temperature dependent, the flame temperature could thus be estimated from the change in current. The flame temperatures calculated from the difference in saturation currents (1734-1887 K) were compared to those from a conventional thermocouple (1580-1908 K). Temperature measurements obtained from tungsten probes placed in rich flames yielded the highest percent error (9.66%-18.70%) due to smaller emission current densities at lower temperatures. The molybdenum probe yielded an accurate temperature value with only 1.29% error. Molybdenum also demonstrated very low probe degradation in comparison to the tungsten probe tips (area reductions of 6% vs. 58%, respectively). The results also show that very little exposure time (<5 s) is needed to obtain a valid ion density measurement and that prolonged flame exposures can yield the flame temperature but also risks damage to the Langmuir probe tip.

The Measurement of Unsteady Surface Pressure Using a Remote Microphone Probe.

PubMed

Guan, Yaoyi; Berntsen, Carl R; Bilka, Michael J; Morris, Scott C

2016-12-03

Microphones are widely applied to measure pressure fluctuations at the walls of solid bodies immersed in turbulent flows. Turbulent motions with various characteristic length scales can result in pressure fluctuations over a wide frequency range. This property of turbulence requires sensing devices to have sufficient sensitivity over a wide range of frequencies. Furthermore, the small characteristic length scales of turbulent structures require small sensing areas and the ability to place the sensors in very close proximity to each other. The complex geometries of the solid bodies, often including large surface curvatures or discontinuities, require the probe to have the ability to be set up in very limited spaces. The development of a remote microphone probe, which is inexpensive, consistent, and repeatable, is described in the present communication. It allows for the measurement of pressure fluctuations with high spatial resolution and dynamic response over a wide range of frequencies. The probe is small enough to be placed within the interior of typical wind tunnel models. The remote microphone probe includes a small, rigid, and hollow tube that penetrates the model surface to form the sensing area. This tube is connected to a standard microphone, at some distance away from the surface, using a "T" junction. An experimental method is introduced to determine the dynamic response of the remote microphone probe. In addition, an analytical method for determining the dynamic response is described. The analytical method can be applied in the design stage to determine the dimensions and properties of the RMP components.

Satellite Based Soil Moisture Product Validation Using NOAA-CREST Ground and L-Band Observations

NASA Astrophysics Data System (ADS)

Norouzi, H.; Campo, C.; Temimi, M.; Lakhankar, T.; Khanbilvardi, R.

2015-12-01

Soil moisture content is among most important physical parameters in hydrology, climate, and environmental studies. Many microwave-based satellite observations have been utilized to estimate this parameter. The Advanced Microwave Scanning Radiometer 2 (AMSR2) is one of many remotely sensors that collects daily information of land surface soil moisture. However, many factors such as ancillary data and vegetation scattering can affect the signal and the estimation. Therefore, this information needs to be validated against some "ground-truth" observations. NOAA - Cooperative Remote Sensing and Technology (CREST) center at the City University of New York has a site located at Millbrook, NY with several insitu soil moisture probes and an L-Band radiometer similar to Soil Moisture Passive and Active (SMAP) one. This site is among SMAP Cal/Val sites. Soil moisture information was measured at seven different locations from 2012 to 2015. Hydra probes are used to measure six of these locations. This study utilizes the observations from insitu data and the L-Band radiometer close to ground (at 3 meters height) to validate and to compare soil moisture estimates from AMSR2. Analysis of the measurements and AMSR2 indicated a weak correlation with the hydra probes and a moderate correlation with Cosmic-ray Soil Moisture Observing System (COSMOS probes). Several differences including the differences between pixel size and point measurements can cause these discrepancies. Some interpolation techniques are used to expand point measurements from 6 locations to AMSR2 footprint. Finally, the effect of penetration depth in microwave signal and inconsistencies with other ancillary data such as skin temperature is investigated to provide a better understanding in the analysis. The results show that the retrieval algorithm of AMSR2 is appropriate under certain circumstances. This validation algorithm and similar study will be conducted for SMAP mission. Keywords: Remote Sensing, Soil Moisture, AMSR2, SMAP, L-Band.

Effects of radiofrequency probe application on irrigation fluid temperature in the wrist joint.

PubMed

Sotereanos, Dean G; Darlis, Nickolaos A; Kokkalis, Zinon T; Zanaros, George; Altman, Gregory T; Miller, Mark Carl

2009-12-01

Radiofrequency (RF) probes used in wrist arthroscopy may raise joint fluid temperature, increasing the risk of capsular and ligamentous damage. The purposes of the current study were to measure joint fluid temperature during wrist arthroscopy with the use of RF probes, and to determine whether using an outlet portal will reduce the maximum temperature. We performed wrist arthroscopy on 8 cadaveric arms. Ablation and coagulation cycles using RF probe were performed at documented locations within the joint. This was done for 60-second intervals on both the radial and ulnar side of the wrist, to mimic clinical practice. We used 4 fiberoptic phosphorescent probes to measure temperature (radial, ulnar, inflow-tube, and outflow-tube probes) and measured joint fluid temperature with and without outflow. There was a significant difference between wrists with and without outflow when examining maximum ablation temperatures (p < .002). All specimens showed higher maximum and average ablation temperatures without outflow. Maximum joint temperatures, greater than 60 degrees C, were observed in only no-outflow conditions. In performing RF ablation during wrist arthroscopy, the use of an outlet portal reduces the joint fluid temperature. Without an outlet portal, maximum temperatures can exceed desirable levels when using ablation; such temperatures have the potential to damage adjacent tissues. It is useful to maintain adequate outflow when using the radiofrequency probes during wrist arthroscopy.

Development of a new mini-invasive tumour hyperthermia probe using high-temperature water vapour.

PubMed

Yu, Tian-Hua; Zhou, Yi-Xin; Liu, Jing

2004-01-01

A new mini-invasive hyperthermia probe using high-temperature water vapour for deep regional tumour treatment was developed in this paper. The vacuum insulation mechanism was incorporated into the probe to avoid heating damage to the normal tissues around the edge of the insertion path. To better understand the heat transfer behaviour in living tissues due to operation of the probe, theoretical models based on the Pennes' equation were established and two closed form analytical solutions under constant flux or temperature heating at the tip of probe were obtained. Parametric studies were performed to investigate the influence of various parameters on the temperature response of tissues heated by the probe. Further, several simulating experiments on the actual heating performance of the probe fabricated in this paper were conducted on the in vitro biological materials (fresh pork) and phantom gel. It was demonstrated that the probe can cause a high enough temperature over the treatment area to thermally destroy the tumour tissue in due time, while the temperature over the surrounding healthy tissues can be kept below a safe threshold value. This mini-invasive heating probe may have significant applications in future clinical tumour hyperthermia.

Secretory phospholipase A2 activity in blood serum: the challenge to sense.

PubMed

Alekseeva, A S; Korotaeva, A A; Samoilova, E V; Volynsky, P E; Vodovozova, E L; Boldyrev, I A

2014-11-07

Excess levels of secretory phospholipase A2 (sPLA2) is known to contribute to several inflammatory diseases including vascular inflammation correlating with coronary events in coronary artery disease. Thus a method to monitor sPLA2 activity in blood serum is urgently needed. Such method is still a challenge since existing fluorescent probes do not allow to monitor sPLA2 activity directly in blood serum. Here we analyze and overcome barriers in sPLA2 sensing methodology and report a fluorescent probe and a kinetic model of its hydrolysis by sPLA2. New probe is designed with a fluorophore and a quencher not interfering binding to the enzyme. At the same time phospholipid matrix bearing the probe promotes efficient initial quenching of the fluorophore. Kinetic model of probe hydrolysis takes into account signal change due to the side processes. The probe and the kinetic model applied together prove the concept that the activity of sPLA can be measured directly in blood serum. Copyright © 2014 Elsevier Inc. All rights reserved.

Differentiating RNA from DNA by a molecular fluorescent probe based on the "door-bolt" mechanism biomaterials.

PubMed

Yao, Qichao; Li, Haidong; Xian, Liman; Xu, Feng; Xia, Jing; Fan, Jiangli; Du, Jianjun; Wang, Jingyun; Peng, Xiaojun

2018-09-01

Although excellent florescent probes have been developed for DNA, good probes for RNA remain lacking. The shortage of reported and commercial RNA probes is attributable to their severe interference from DNA. As DNA and RNA have similar structures but different functions, it has been an imperative challenge to develop RNA probes that differentiate from DNA. In this study, an NIR fluorescent probe, NBE, is described, which contains a bulky julolidine group that can fit in a spacious RNA pocket and emit intense fluorescence. However, NBE has no response to DNA, as it cannot intercalate into the double strands or even in the DNA minor groove. The sensing mechanism is similar to the effect of a door-bolt. NBE shows excellent performance in RNA sensing (outstanding photostability, high selectivity and fast response), whether in aqueous buffers, fixed cells or living cells. These findings might provide not only a potential imaging tool but also a new design strategy for the recognition of RNA while avoiding interference from DNA. Copyright © 2018 Elsevier Ltd. All rights reserved.

A temperature sensor based on the enhanced upconversion luminescence of Li+ doped NaLuF4:Yb3+,Tm3+/Er3+ nano/microcrystals.

PubMed

Qiang, Qinping; Du, Shanshan; Ma, Xinlong; Chen, Wenbo; Zhang, Gangyi; Wang, Yuhua

2018-05-09

In this paper, fluorescent and optical temperature sensing bi-functional Li+-doping NaLuF4:Ln (Ln = Yb3+, Tm3+/Er3+) nanocrystals were synthesized via a simple hydrothermal method using oleic acid as a capping ligand. The crystal phase, size, upconversion (UC) properties, and optical temperature sensing characteristics of the crystals can be easily modified by Li+ doping. The results reveal that additional Li+ can promote the transformation from the hexagonal phase to the cubic phase and reduce the size of the nanocrystals. In addition, NaLuF4:Ln (Ln = Yb3+, Tm3+, Li+) nanocrystals present efficient near infrared (NIR) emission, which is beneficial for in vivo biomedical applications due to the increased penetration depth and low radiation damage of NIR light in bio-tissues. More importantly, under 980 nm excitation, the temperature dependent UCL from the 2H11/2 and 4S3/2 levels of Er3+ ions in NaLuF4:Yb3+,Er3+,Li+ microcrystals was investigated systematically. The fluorescence intensity ratios (FIR) of the pairs of thermally coupled levels were studied as a function of temperature in the range of 298-523 K. The maximum sensor sensitivities were found to be about 0.0039 K-1 (523 K) by exploiting the UC emissions from the 2H11/2 and 4S3/2 levels. This suggests that the Li+-doped upconversion luminescence (UCL) materials are promising prototypes for application as multi-mode probes for use in bio-separation and optical thermometers.

A compact, high temperature nuclear magnetic resonance probe for use in a narrow-bore superconducting magnet

NASA Astrophysics Data System (ADS)

Adler, Stuart B.; Michaels, James N.; Reimer, Jeffrey A.

1990-11-01

The design of a nuclear magnetic resonance (NMR) probe is reported, that can be used in narrow-bore superconducting solenoids for the observation of nuclear induction at high temperatures. The probe is compact, highly sensitive, and stable in continuous operation at temperatures up to 1050 C. The essential feature of the probe is a water-cooled NMR coil that contains the sample-furnace; this design maximizes sensitivity and circuit stability by maintaining the probe electronics at ambient temperature. The design is demonstrated by showing high temperature O-17 NMR spectra and relaxation measurements in solid barium bismuth oxide and yttria-stabilized zirconia.

Resolving an anomaly in electron temperature measurement using double and triple Langmuir probes

NASA Astrophysics Data System (ADS)

Ghosh, Soumen; Barada, K. K.; Chattopadhyay, P. K.; Ghosh, J.; Bora, D.

2015-02-01

Langmuir probes with variants such as single, double and triple probes remain the most common method of electron temperature measurement in low-temperature laboratory plasmas. However, proper estimation of electron temperature mainly using triple probe configuration requires the proper choice of compensation factor (W). Determination of the compensating factor is not very straightforward as it depends heavily on plasma floating potential (Vf), electron temperature (Te), the type of gas used for plasma production and the bias voltage applied to probe pins, especially in cases where there are substantial variations in floating potential. In this paper we highlight the anomaly in electron temperature measurement using double and triple Langmuir probe techniques as well as the proper determination of the compensation factor (W) to overcome this anomaly. Experiments are carried out with helicon antenna producing inductive radiofrequency plasmas, where significant variation of floating potential along the axis enables a detailed study of deviations introduced in Te measurements using triple probes compared to double and single probes. It is observed that the bias voltage between the probe pins of the triple probes plays an important role in the accurate determination of the compensating factor (W) and should be in the range (5Vd2 < Vd3 < 10Vd2), where Vd2 and Vd3 are the voltage between floating probe pins 2 and 1 and the bias voltage, respectively.

Energy Donor Effect on the Sensing Performance for a Series of FRET-Based Two-Photon Fluorescent Hg2+ Probes.

PubMed

Zhang, Yujin; Hu, Wei

2017-01-25

Nonlinear optical properties of a series of newly-synthesized molecular fluorescent probes for Hg 2+ containing the same acceptor (rhodamine group) are analyzed by using time-dependent density functional theory in combination with analytical response theory. Special emphasis is placed on evolution of the probes' optical properties in the absence and presence of Hg 2+ . These compounds show drastic changes in their photoabsorption and photoemission properties when they react with Hg 2+ , indicating that they are excellent candidates for ratiometric and colorimetric fluorescent chemosensors. Most importantly, the energy donor moiety is found to play a dominant role in sensing performance of these probes. Two-photon absorption cross sections of the compounds are increased with the presence of Hg 2+ , which theoretically suggests the possibility of the probes to be two-photon fluorescent Hg 2+ sensors. Moreover, analysis of molecular orbitals is presented to explore responsive mechanism of the probes, where the fluorescence resonant energy transfer process is theoretically demonstrated. Our results elucidate the available experimental measurements. This work provides guidance for designing efficient two-photon fluorescent probes that are geared towards biological and chemical applications.

Remote Sensing Measurements of the Corona with the Solar Probe

NASA Technical Reports Server (NTRS)

Habbal, Shadia Rifai; Woo, Richard

1996-01-01

Remote sensing measurements of the solar corona are indespensible for the exploration of the source and acceleration regions of the solar wind which are inaccessible to in situ plasma, paritcles and field experiments.Furthermore, imaging the solar disk and coronal from the unique vantage point of the trajectory and the proximity of the Solar Probe spacecraft, will provide the first ever opportunity to explore the small scale structures within coronal holes and streamers from viewing angles and with spatial resolutions never attained before.

Nitric oxide sensing by chlorophyll a.

PubMed

Bhattacharya, Abhishek; Biswas, Pranjal; Kar, Puranjoy; Roychoudhury, Piya; Basu, Sankar; Ganguly, Souradipta; Ghosh, Sanjay; Panda, Koustubh; Pal, Ruma; Dasgupta, Anjan Kr

2017-09-08

Nitric oxide (NO) acts as a signalling molecule that has direct and indirect regulatory roles in various functional processes in biology, though in plant kingdom its role is relatively unexplored. One reason for this is the fact that sensing of NO is always challenging. There are very few probes that can classify the different NO species. The present paper proposes a simple but straightforward way for sensing different NO species using chlorophyll, the source of inspiration being hemoglobin that serves as NO sink in mammalian systems. The proposed method is able to classify NO from DETA-NONOate or (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl) amino] diazen-1-ium-1,2-diolate, nitrite, nitrate and S-nitrosothiol or SNO. This discrimination is carried out by chlorophyll a (chl a) at nano molar (nM) order of sensitivity and at 293 K-310 K. Molecular docking reveals the differential binding effects of NO and SNO with chlorophyll, the predicted binding affinity matching with the experimental observation. Additional experiments with a diverse range of cyanobacteria reveal that apart from the spectroscopic approach the proposed sensing module can be used in microscopic inspection of NO species. Binding of NO is sensitive to temperature and static magnetic field. This provides additional support for the involvement of the porphyrin ring structures to the NO sensing process. This also, broadens the scope of the sensing methods as hinted in the text. Copyright © 2017 Elsevier B.V. All rights reserved.

Cyclic coding for Brillouin optical time-domain analyzers using probe dithering.

PubMed

Iribas, Haritz; Loayssa, Alayn; Sauser, Florian; Llera, Miguel; Le Floch, Sébastien

2017-04-17

We study the performance limits of mono-color cyclic coding applied to Brillouin optical time-domain analysis (BOTDA) sensors that use probe wave dithering. BOTDA analyzers with dithering of the probe use a dual-probe-sideband setup in which an optical frequency modulation of the probe waves along the fiber is introduced. This avoids non-local effects while keeping the Brillouin threshold at its highest level, thus preventing the spontaneous Brillouin scattering from generating noise in the deployed sensing fiber. In these conditions, it is possible to introduce an unprecedented high probe power into the sensing fiber, which leads to an enhancement of the signal-to-noise ratio (SNR) and consequently to a performance improvement of the analyzer. The addition of cyclic coding in these set-ups can further increase the SNR and accordingly enhance the performance. However, this unprecedented probe power levels that can be employed result in the appearance of detrimental effects in the measurement that had not previously been observed in other BOTDA set-ups. In this work, we analyze the distortion in the decoding process and the errors in the measurement that this distortion causes, due to three factors: the power difference of the successive pulses of a code sequence, the appearance of first-order non-local effects and the non-linear amplification of the probe wave that results when using mono-color cyclic coding of the pump pulses. We apply the results of this study to demonstrate the performance enhancement that can be achieved in a long-range dithered dual-probe BOTDA. A 164-km fiber-loop is measured with 1-m spatial resolution, obtaining 3-MHz Brillouin frequency shift measurement precision at the worst contrast location. To the best of our knowledge, this is the longest sensing distance achieved with a BOTDA sensor using mono-color cyclic coding.

Nanocomposite thin films for optical temperature sensing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ohodnicki, Jr., Paul R.; Brown, Thomas D.; Buric, Michael P.

2017-02-14

The disclosure relates to an optical method for temperature sensing utilizing a temperature sensing material. In an embodiment the gas stream, liquid, or solid has a temperature greater than about 500.degree. C. The temperature sensing material is comprised of metallic nanoparticles dispersed in a dielectric matrix. The metallic nanoparticles have an electronic conductivity greater than approximately 10.sup.-1 S/cm at the temperature of the temperature sensing material. The dielectric matrix has an electronic conductivity at least two orders of magnitude less than the dispersed metallic nanoparticles at the temperature of the temperature sensing material. In some embodiments, the chemical composition ofmore » a gas stream or liquid is simultaneously monitored by optical signal shifts through multiple or broadband wavelength interrogation approaches. In some embodiments, the dielectric matrix provides additional functionality due to a temperature dependent band-edge, an optimized chemical sensing response, or an optimized refractive index of the temperature sensing material for integration with optical waveguides.« less

Compact system with handheld microfabricated optoelectronic probe for needle-based tissue sensing applications

NASA Astrophysics Data System (ADS)

Lee, Seung Yup; Na, Kyounghwan; Pakela, Julia M.; Scheiman, James M.; Yoon, Euisik; Mycek, Mary-Ann

2017-02-01

We present the design, development, and bench-top verification of an innovative compact clinical system including a miniaturized handheld optoelectronic sensor. The integrated sensor was microfabricated with die-level light-emitting diodes and photodiodes and fits into a 19G hollow needle (internal diameter: 0.75 mm) for optical sensing applications in solid tissues. Bench-top studies on tissue-simulating phantoms have verified system performance relative to a fiberoptic based tissue spectroscopy system. With dramatically reduced system size and cost, the technology affords spatially configurable designs for optoelectronic light sources and detectors, thereby enabling customized sensing configurations that would be impossible to achieve with needle-based fiber-optic probes.

Fiber Optic Surface Plasmon Resonance-Based Biosensor Technique: Fabrication, Advancement, and Application.

PubMed

Liang, Gaoling; Luo, Zewei; Liu, Kunping; Wang, Yimin; Dai, Jianxiong; Duan, Yixiang

2016-05-03

Fiber optic-based biosensors with surface plasmon resonance (SPR) technology are advanced label-free optical biosensing methods. They have brought tremendous progress in the sensing of various chemical and biological species. This review summarizes four sensing configurations (prism, grating, waveguide, and fiber optic) with two ways, attenuated total reflection (ATR) and diffraction, to excite the surface plasmons. Meanwhile, the designs of different probes (U-bent, tapered, and other probes) are also described. Finally, four major types of biosensors, immunosensor, DNA biosensor, enzyme biosensor, and living cell biosensor, are discussed in detail for their sensing principles and applications. Future prospects of fiber optic-based SPR sensor technology are discussed.

Technique for temperature compensation of eddy-current proximity probes

NASA Technical Reports Server (NTRS)

Masters, Robert M.

1989-01-01

Eddy-current proximity probes are used in turbomachinery evaluation testing and operation to measure distances, primarily vibration, deflection, or displacment of shafts, bearings and seals. Measurements of steady-state conditions made with standard eddy-current proximity probes are susceptible to error caused by temperature variations during normal operation of the component under investigation. Errors resulting from temperature effects for the specific probes used in this study were approximately 1.016 x 10 to the -3 mm/deg C over the temperature range of -252 to 100 C. This report examines temperature caused changes on the eddy-current proximity probe measurement system, establishes their origin, and discusses what may be done to minimize their effect on the output signal. In addition, recommendations are made for the installation and operation of the electronic components associated with an eddy-current proximity probe. Several techniques are described that provide active on-line error compensation for over 95 percent of the temperature effects.

Evaluation of water cooled supersonic temperature and pressure probes for application to 1366 K flows

NASA Technical Reports Server (NTRS)

Lagen, Nicholas; Seiner, John M.

1990-01-01

Water cooled supersonic probes are developed to investigate total pressure, static pressure, and total temperature in high-temperature jet plumes and thereby determine the mean flow properties. Two probe concepts, designed for operation at up to 1366 K in a Mach 2 flow, are tested on a water cooled nozzle. The two probe designs - the unsymmetric four-tube cooling configuration and the symmetric annular cooling design - take measurements at 755, 1089, and 1366 K of the three parameters. The cooled total and static pressure readings are found to agree with previous test results with uncooled configurations. The total-temperature probe, however, is affected by the introduction of water coolant, and effect which is explained by the increased heat transfer across the thermocouple-bead surface. Further investigation of the effect of coolant on the temperature probe is proposed to mitigate the effect and calculate more accurate temperatures in jet plumes.

Influence of Donor on the Sensing Performance of a Series of Through-Bond Energy Transfer-Based Two-photon Fluorescent Cu(2+) Probes.

PubMed

Zhang, Yu-Jin; Wang, Xin; Zhou, Yong; Wang, Chuan-Kui

2016-07-01

Optical properties of a series of molecular two-photon fluorescent Cu(2+) probes containing the same acceptor (rhodamine group) are analyzed using time-dependent density functional theory in combination with analytical response theory. Special emphasis is placed on evolution of the probes' optical properties in the presence of Cu(2+) . In this study, the compound with naphthalene as the donor is shown to be excellent ratiometric fluorescent chemosensor, whereas the compound with quinoline derivative as the donor shows off/on-typed colorimetric fluorescent response. For the compound with naphthalimide derivative as the donor, changing the connection between the donor and acceptor can efficiently prevent the fluorescent quenching of the probe both in the absence and presence of Cu(2+) . The donor moiety and the connection between donor and acceptor are thus found to play dominant roles on sensing performance of these probes. Moreover, distributions of molecular orbitals involved in the excitation and emission of the probes are analyzed to explore responsive mechanism of the probes. The through-bond energy transfer process is theoretically demonstrated. Our results are used to elucidate the available experimental measurements. This work is helpful to understand the relationships of structure with optical properties for the studied probes. © 2016 The American Society of Photobiology.

Number-unconstrained quantum sensing

NASA Astrophysics Data System (ADS)

Mitchell, Morgan W.

2017-12-01

Quantum sensing is commonly described as a constrained optimization problem: maximize the information gained about an unknown quantity using a limited number of particles. Important sensors including gravitational wave interferometers and some atomic sensors do not appear to fit this description, because there is no external constraint on particle number. Here, we develop the theory of particle-number-unconstrained quantum sensing, and describe how optimal particle numbers emerge from the competition of particle-environment and particle-particle interactions. We apply the theory to optical probing of an atomic medium modeled as a resonant, saturable absorber, and observe the emergence of well-defined finite optima without external constraints. The results contradict some expectations from number-constrained quantum sensing and show that probing with squeezed beams can give a large sensitivity advantage over classical strategies when each is optimized for particle number.

Tensiometer, drive probe for use with environmental testing equipment, and methods of inserting environmental testing equipment into a sample

DOEpatents

Hubbell, Joel M.; Sisson, James B.

2005-07-26

A method of inserting a tensiometer into a sample, comprises providing a drive probe configured to be engaged by direct push equipment; supporting a porous member from the drive probe; and driving the drive probe into the sample using a cone penetrometer. A tensiometer comprises a drive probe configured to be engaged by direct push equipment or a cone penetrometer; a porous member supported by the drive probe; and a pressure sensor in pressure sensing relation to the porous member.

New Optical Sensing Materials for Application in Marine Research

NASA Astrophysics Data System (ADS)

Borisov, S.; Klimant, I.

2012-04-01

Optical chemosensors are versatile analytical tools which find application in numerous fields of science and technology. They proved to be a promising alternative to electrochemical methods and are applied increasingly often in marine research. However, not all state-of-the- art optical chemosensors are suitable for these demanding applications since they do not fully fulfil the requirements of high luminescence brightness, high chemical- and photochemical stability or their spectral properties are not adequate. Therefore, development of new advanced sensing materials is still of utmost importance. Here we present a set of novel optical sensing materials recently developed in the Institute of Analytical Chemistry and Food Chemistry which are optimized for marine applications. Particularly, we present new NIR indicators and sensors for oxygen and pH which feature high brightness and low level of autofluorescence. The oxygen sensors rely on highly photostable metal complexes of benzoporphyrins and azabenzoporphyrins and enable several important applications such as simultaneous monitoring of oxygen and chlorophyll or ultra-fast oxygen monitoring (Eddy correlation). We also developed ulta-sensitive oxygen optodes which enable monitoring in nM range and are primary designed for investigation of oxygen minimum zones. The dynamic range of our new NIR pH indicators based on aza-BODIPY dyes is optimized for the marine environment. A highly sensitive NIR luminescent phosphor (chromium(III) doped yttrium aluminium borate) can be used for non-invasive temperature measurements. Notably, the oxygen, pH sensors and temperature sensors are fully compatible with the commercially available fiber-optic readers (Firesting from PyroScience). An optical CO2 sensor for marine applications employs novel diketopyrrolopyrrol indicators and enables ratiometric imaging using a CCD camera. Oxygen, pH and temperature sensors suitable for lifetime and ratiometric imaging of analytes distribution are also realized. To enable versatility of applications we also obtained a range of nano- and microparticles suitable for intra- and extracellular imaging of the above analytes. Bright ratiometric 2-photon-excitable probes were also developed. Magnetic microparticles are demonstrated to be very promising tools for imaging of oxygen, temperature and other parameters in biofilms, corals etc. since they combine the sensing function with the possibility of external manipulation.

Monodisperse Magneto-Fluorescent Bifunctional Nanoprobes for Bioapplications

NASA Astrophysics Data System (ADS)

Zhang, Hongwang; Huang, Heng; Pralle, Arnd; Zeng, Hao

2013-03-01

We present the work on the synthesis of dye-doped monodisperse Fe/SiO2 core/shell nanoparticles as bifunctional probes for bioapplications. Magnetic nanoparticles (NP) have been widely studied as nano-probes for bio-imaging, sensing as well as for cancer therapy. Among all the NPs, Fe NPs have been the focus because they have very high magnetization. However, Fe NPs are usually not stable in ambient due to the fast surface oxidation of the NPs. On the other hand, dye molecules have long been used as probes for bio-imaging. But they are sensitive to environmental conditions. It requires passivation for both so that they can be stable for applications. In this work, monodisperse Fe NPs with sizes ranging from 13-20 nm have been synthesized through the chemical thermal-decomposition in a solution. Silica shells were then coated on the Fe NPs by a two-phase oil-in-water method. Dye molecules were first bonded to a silica precursor and then encapsulated into the silica shell during the coating process. The silica shells protect both the Fe NPs and dye molecules, which makes them as robust probes. The dye doped Fe/SiO2 core/shell NPs remain both highly magnetic and highly fluorescent. The stable dye doped Fe/SiO2NPs have been used as a dual functional probe for both magnetic heating and local nanoscale temperature sending, and their performance will be reported. Research supported by NSF DMR 0547036, DMR1104994.

Noncontact Measurement Of Sizes And Eccentricities Of Holes

NASA Technical Reports Server (NTRS)

Chern, Engmin J.

1993-01-01

Semiautomatic eddy-current-probe apparatus makes noncontact measurements of nominally round holes in electrically conductive specimens and processes measurement data into diameters and eccentricities of holes. Includes x-y translation platform, which holds specimen and moves it horizontally. Probe mounted on probe scanner, positioning probe along vertical (z) direction and rotates probe about vertical axis at preset low speed. Eddy-current sensing coil mounted in side of probe near tip. As probe rotates, impedance analyzer measures electrical impedance (Z) of coil as function of instantaneous rotation angle. Translation and rotation mechanisms and impedance analyzer controlled by computer, which also processes impedance-measurement data.

Xanthine oxidase functionalized Ta2O5 nanostructures as a novel scaffold for highly sensitive SPR based fiber optic xanthine sensor.

PubMed

Kant, Ravi; Tabassum, Rana; Gupta, Banshi D

2018-01-15

Fabrication and characterization of a surface plasmon resonance based fiber optic xanthine sensor using entrapment of xanthine oxidase (XO) enzyme in several nanostructures of tantalum (v) oxide (Ta 2 O 5 ) have been reported. Chemical route was adopted for synthesizing Ta 2 O 5 nanoparticles, nanorods, nanotubes and nanowires while Ta 2 O 5 nanofibers were prepared by electrospinning technique. The synthesized Ta 2 O 5 nanostructures were characterized by photoluminescence, scanning electron microscopy, UV-Visible spectra and X-ray diffraction pattern. The probes were fabricated by coating an unclad core of the fiber with silver layer followed by the deposition of XO entrapped Ta 2 O 5 nanostructures. The crux of sensing mechanism relies on the modification of dielectric function of sensing layer upon exposure to xanthine solution of diverse concentrations, reflected in terms of shift in resonance wavelength. The sensing probe coated with XO entrapped Ta 2 O 5 nanofibers has been turned out to possess maximum sensitivity amongst the synthesized nanostructures. The probe was optimized in terms of pH of the sample and the concentration of XO entrapped in Ta 2 O 5 nanofibers. The optimized sensing probe possesses a remarkably good sensitivity of 26.2nm/µM in addition to linear range from 0 to 3µM with an invincible LOD value of 0.0127µM together with a response time of 1min. Furthermore, probe selectivity with real sample analysis ensure the usage of the sensor for practical scenario. The results reported open a novel perspective towards a sensitive, rapid, reliable and selective detection of xanthine. Copyright © 2017 Elsevier B.V. All rights reserved.

Excitation and emission wavelength ratiometric cyanide-sensitive probes for physiological sensing.

PubMed

Badugu, Ramachandram; Lakowicz, Joseph R; Geddes, Chris D

2004-04-01

We characterize three new fluorescent probes that show both spectral shifts and intensity changes in the presence of aqueous cyanide, allowing for both excitation and fluorescence emission wavelength ratiometric and colorimetric sensing. The relatively high binding constants of the probes for cyanide enables a distinct colorimetric change to be visually observed with as little as 10 microM cyanide. The response of the new probes is based on the ability of the boronic acid group to interact with the CN(-) anion, changing from the neutral form of the boronic acid group R-B(OH)(2) to the anionic R-B(-)(OH)3 form, which is an electron-donating group. The presence of an electron-deficient quaternary heterocyclic nitrogen center and a strong electron-donating amino group in the 6 position on the quinolinium backbone provides for the spectral changes observed upon CN(-) complexation. We have determined the binding constants for the ortho-, meta-, and para-boronic acid probes to be 0.12, 0.17, and 0.14 microM(-3). In addition we have synthesized a control compound that does not contain the boronic acid moiety, allowing for structural comparisons and a rationale for the sensing mechanism to be made. Finally we show that the affinity for monosaccharides, such as glucose or fructose, is relatively low as compared to that for cyanide, enabling the potential detection of cyanide in physiologies up to lethal levels.

Photon Shot Noise Limited Radio Frequency Electric Field Sensing Using Rydberg Atoms in Vapor Cells

NASA Astrophysics Data System (ADS)

Kumar, Santosh; Jahangiri, Akbar J.; Fan, Haoquan; Kuebler, Harald; Shaffer, James P.

2017-04-01

We report Rydberg atom-based radio frequency (RF) electrometry measurements at a sensitivity limited by probe laser photon shot noise. By utilizing the phenomena of electromagnetically induced transparency (EIT) in room temperature atomic vapor cells, Rydberg atoms can be used for absolute electric field measurements that significantly surpass conventional methods in utility, sensitivity and accuracy. We show that by using a Mach-Zehnder interferometer with homodyne detection or using frequency modulation spectroscopy with active control of residual amplitude modulation we can achieve a RF electric field detection sensitivity of 3 μVcm-1Hz/2. The sensitivity is limited by photon shot noise on the detector used to readout the probe laser of the EIT scheme. We suggest a new multi-photon scheme that can mitigate the effect of photon shot noise. The multi-photon approach allows an increase in probe laser power without decreasing atomic coherence times that result from collisions caused by an increase in Rydberg atom excitation. The multi-photon scheme also reduces Residual Doppler broadening enabling more accurate measurements to be carried out. This work is supported by DARPA, and NRO.

Nanoparticle assembled microcapsules for application as pH and ammonia sensor.

PubMed

Amali, Arlin Jose; Awwad, Nour H; Rana, Rohit Kumar; Patra, Digambara

2011-12-05

The encapsulation of molecular probes in a suitable nanostructured matrix can be exploited to alter their optical properties and robustness for fabricating efficient chemical sensors. Despite high sensitivity, simplicity, selectivity and cost effectiveness, the photo-destruction and photo-bleaching are the serious concerns while utilizing molecular probes. Herein we demonstrate that hydroxy pyrene trisulfonate (HPTS), a pH sensitive molecular probe, when encapsulated in a microcapsule structure prepared via the assembly of silica nanoparticles mediated by poly-L-lysine and trisodium citrate, provides a robust sensing material for pH sensing under the physiological conditions. The temporal evolution under continuous irradiation indicates that the fluorophore inside the silica microcapsule is extraordinarily photostable. The fluorescence intensity alternation at dual excitation facilitates for a ratiometic sensing of the pH, however, the fluorescence lifetime is insensitive to hydrogen ion concentration. The sensing scheme is found to be robust, fast and simple for the measurement of pH in the range 5.8-8.0, and can be successfully applied for the determination of ammonia in the concentration range 0-1.2 mM, which is important for aquatic life and the environment. Copyright © 2011 Elsevier B.V. All rights reserved.

The new Licox combined brain tissue oxygen and brain temperature monitor: assessment of in vitro accuracy and clinical experience in severe traumatic brain injury.

PubMed

Stewart, Campbell; Haitsma, Iain; Zador, Zsolt; Hemphill, J Claude; Morabito, Diane; Manley, Geoffrey; Rosenthal, Guy

2008-12-01

Monitoring of brain tissue oxygen tension is increasingly being used to monitor patients after severe traumatic brain injury and to guide therapies aimed at maintaining brain tissue oxygen tension above threshold levels. The new Licox PMO combined oxygen and temperature catheter (Integra LifeSciences, Plainsboro, NJ) combines measurements of oxygen tension and temperature in a single probe inserted through a bolt mechanism. In this study, we sought to evaluate the accuracy of the new Licox PMO probe under controlled laboratory conditions and to assess the accuracy of oxygen tension and temperature measurements and the new automated card calibration system. We also describe our clinical experience with the Licox PMO probe. Oxygen tension was measured in a 2-chambered apparatus at different oxygen tensions and temperatures. The new card calibration system was compared with a manually calibrated system. Rates of hematoma, infection, and dislodgement in our clinical experience were recorded. The new Licox PMO probe accurately measures oxygen tension over a wide range of oxygen concentrations and physiological temperatures, but it does have a small tendency to underestimate oxygen tension (mean error, -3.8 +/- 3.5%) that is more pronounced between the temperatures of 33 and 39 degrees C. The thermistor of the PMO probe also has a tendency to underestimate temperature when compared with a resistance thermometer (mean error, -0.67 +/- 0.22 degrees C). The card calibration system was also found to introduce some variability in measurements of oxygen tension when compared with a manually calibrated system. Clinical experience with the new probe indicates good placement within the white matter using the improved bolt system and low rates of hematoma (2.9%), infection (0%), and dislodgement (5.9%). The new Licox PMO probe is accurate but has a small, consistent tendency to under-read oxygen tension that is more pronounced at higher temperatures. The probe tends to under-read temperature by 0.5 to 0.8 degrees C across temperatures, suggesting that caution should be used when brain temperature is measured with the Licox PMO probe and used to guide temperature-directed treatment strategies. The Licox PMO probe improves upon previous models in allowing consistent and accurate placement in the white matter and obviating the need for placement of 2 separate probes to measure oxygen tension and temperature.

Eddy Current Probe for Surface and Sub-Surface Inspection

NASA Technical Reports Server (NTRS)

Wincheski, Russell A. (Inventor); Simpson, John W. (Inventor)

2014-01-01

An eddy current probe includes an excitation coil for coupling to a low-frequency alternating current (AC) source. A magneto-resistive sensor is centrally disposed within and at one end of the excitation coil to thereby define a sensing end of the probe. A tubular flux-focusing lens is disposed between the excitation coil and the magneto-resistive sensor. An excitation wire is spaced apart from the magneto-resistive sensor in a plane that is perpendicular to the sensor's axis of sensitivity and such that, when the sensing end of the eddy current probe is positioned adjacent to the surface of a structure, the excitation wire is disposed between the magneto-resistive sensor and the surface of the structure. The excitation wire is coupled to a high-frequency AC source. The excitation coil and flux-focusing lens can be omitted when only surface inspection is required.

Recent Advances in Macrocyclic Fluorescent Probes for Ion Sensing.

PubMed

Wong, Joseph K-H; Todd, Matthew H; Rutledge, Peter J

2017-01-25

Small-molecule fluorescent probes play a myriad of important roles in chemical sensing. Many such systems incorporating a receptor component designed to recognise and bind a specific analyte, and a reporter or transducer component which signals the binding event with a change in fluorescence output have been developed. Fluorescent probes use a variety of mechanisms to transmit the binding event to the reporter unit, including photoinduced electron transfer (PET), charge transfer (CT), Förster resonance energy transfer (FRET), excimer formation, and aggregation induced emission (AIE) or aggregation caused quenching (ACQ). These systems respond to a wide array of potential analytes including protons, metal cations, anions, carbohydrates, and other biomolecules. This review surveys important new fluorescence-based probes for these and other analytes that have been reported over the past five years, focusing on the most widely exploited macrocyclic recognition components, those based on cyclam, calixarenes, cyclodextrins and crown ethers; other macrocyclic and non-macrocyclic receptors are also discussed.

Aminoquinoline based highly sensitive fluorescent sensor for lead(II) and aluminum(III) and its application in live cell imaging.

PubMed

Anand, Thangaraj; Sivaraman, Gandhi; Mahesh, Ayyavu; Chellappa, Duraisamy

2015-01-01

We have synthesized a new probe 5-((anthracen-9-ylmethylene) amino)quinolin-10-ol (ANQ) based on anthracene platform. The probe was tested for its sensing behavior toward heavy metal ions Hg(2+), Pb(2+), light metal Al(3+) ion, alkali, alkaline earth, and transition metal ions by UV-visible and fluorescent techniques in ACN/H2O mixture buffered with HEPES (pH 7.4). It shows high selectivity toward sensing Pb(2+)/Al(3+) metal ions. Importantly, 10-fold and 5- fold fluorescence enhancement at 429 nm was observed for probe upon complexation with Pb(2+) and Al(3+) ions, respectively. This fluorescence enhancement is attributable to the prevention of photoinduced electron transfer. The photonic studies indicate that the probe can be adopted as a sensitive fluorescent chemosensor for Pb(2+) and Al(3+) ions. Copyright © 2014 Elsevier B.V. All rights reserved.

A highly selective and ratiometric fluorescent probe for cyanide by rationally altering the susceptible H-atom.

PubMed

Hao, Yuanqiang; Nguyen, Khac Hong; Zhang, Yintang; Zhang, Guan; Fan, Shengnan; Li, Fen; Guo, Chao; Lu, Yuanyuan; Song, Xiaoqing; Qu, Peng; Liu, You-Nian; Xu, Maotian

2018-01-01

A highly selective and ratiometric fluorescent probe for cyanide was rationally designed and synthesized. The probe comprises a fluorophore unit of naphthalimide and a CN - acceptor of methylated trifluoroacetamide group. For these previous reported trifluoroacetamide derivative-based cyanide chemosensors, the H-atom of amide adjacent to trifluoroacetyl group is susceptible to be attacked by various anions (CN - itself, F - , AcO - , et al.) and even the solvent molecule, which resulted in the bewildered reaction mechanism and poor selectivity of the assay. In this work, the susceptible H-atom of trifluoroacetamide was artfully substituted by alkyl group. Thus a highly specific fluorescent probe was developed for cyanide sensing. Upon the nucleophilic addition of cyanide anion to the carbonyl of trifluoroacetamide moiety of the probe, the ICT process of the probe was significantly enhanced and leading to a remarkable red shift in both absorption and emission spectra of the probe. This fluorescent assay showed a linear range of 1.0-80.0µM and a LOD (limit of detection) of 0.23µM. All the investigated interference have no influence on the sensing behavior of the probe toward cyanide. Moreover, by coating on TLC plate, the probe can be utilized for practical detection of trace cyanide in water samples. Copyright © 2017. Published by Elsevier B.V.

Detection of single-nucleotide polymorphisms using an ON-OFF switching of regenerated biosensor based on a locked nucleic acid-integrated and toehold-mediated strand displacement reaction.

PubMed

Gao, Zhong Feng; Ling, Yu; Lu, Lu; Chen, Ning Yu; Luo, Hong Qun; Li, Nian Bing

2014-03-04

Although various strategies have been reported for single-nucleotide polymorphisms (SNPs) detection, development of a time-saving, specific, and regenerated electrochemical sensing platform still remains a realistic goal. In this study, an ON-OFF switching of a regenerated biosensor based on a locked nucleic acid (LNA)-integrated and toehold-mediated strand displacement reaction technique is constructed for detection of SNPs. The LNA-integrated and methylene blue-labeled capture probe with an external toehold is designed to switch on the sensing system. The mutant-type DNA probe completes complementary with the capture probe to trigger the strand displacement reaction, which switches off the sensing system. However, when the single-base mismatched wild-type DNA probe is presented, the strand displacement reaction cannot be achieved; therefore, the sensing system still keeps the ON state. This DNA sensor is stable over five reuses. We further testify that the LNA-integrated sequence has better recognition ability for SNPs detection compared to the DNA-integrated sequence. Moreover, this DNA senor exhibits a remarkable discrimination capability of SNPs among abundant wild-type targets and 6000-fold (m/m) excess of genomic DNA. In addition, it is selective enough in complex and contaminant-ridden samples, such as human urine, soil, saliva, and beer. Overall, these results demonstrate that this reliable DNA sensor is easy to be fabricated, simple to operate, and stable enough to be readily regenerated.

Dual-Modal Colorimetric/Fluorescence Molecular Probe for Ratiometric Sensing of pH and Its Application.

PubMed

Wu, Luling; Li, Xiaolin; Huang, Chusen; Jia, Nengqin

2016-08-16

As traditional pH meters cannot work well for minute regions (such as subcellular organelles) and in harsh media, molecular pH-sensitive devices for monitoring pH changes in diverse local heterogeneous environments are urgently needed. Here, we report a new dual-modal colorimetric/fluorescence merocyanine-based molecular probe (CPH) for ratiometric sensing of pH. Compared with previously reported pH probes, CPH bearing the benzyl group at the nitrogen position of the indolium group and the phenol, which is used as the acceptor for proton, could respond to pH changes immediately through both the ratiometric fluorescence signal readout and naked-eye colorimetric observation. The sensing process was highly stable and reversible. Most importantly, the suitable pKa value (6.44) allows CPH to presumably accumulate in lysosomes and become a lysosome-target fluorescent probe. By using CPH, the intralysosomal pH fluctuation stimulated by antimalaria drug chloroquine was successfully tracked in live cells through the ratiometric fluorescence images. Additionally, CPH could be immobilized on test papers, which exhibited a rapid and reversible colorimetric response to acid/base vapor through the naked-eye colorimetric analysis. This proof-of-concept study presents the potential application of CPH as a molecular tool for monitoring intralysosomal pH fluctuation in live cells, as well as paves the way for developing the economic, reusable, and fast-response optical pH meters for colorimetric sensing acid/base vapor with direct naked-eye observation.

Reflection based Extraordinary Optical Transmission Fiber Optic Probe for Refractive Index Sensing.

PubMed

Lan, Xinwei; Cheng, Baokai; Yang, Qingbo; Huang, Jie; Wang, Hanzheng; Ma, Yinfa; Shi, Honglan; Xiao, Hai

2014-03-31

Fiber optic probes for chemical sensing based on the extraordinary optical transmission (EOT) phenomenon are designed and fabricated by perforating subwavelength hole arrays on the gold film coated optical fiber endface. The device exhibits a red shift in response to the surrounding refractive index increases with high sensitivity, enabling a reflection-based refractive index sensor with a compact and simple configuration. By choosing the period of hole arrays, the sensor can be designed to operate in the near infrared telecommunication wavelength range, where the abundant source and detectors are available for easy instrumentation. The new sensor probe is demonstrated for refractive index measurement using refractive index matching fluids. The sensitivity reaches 573 nm/RIU in the 1.333~1.430 refractive index range.

Mass sensing based on a circuit cavity electromechanical system

NASA Astrophysics Data System (ADS)

Jiang, Cheng; Chen, Bin; Li, Jin-Jin; Zhu, Ka-Di

2011-10-01

We present a scheme for mass sensing based on a circuit cavity electromechanical system where a free-standing, flexible aluminium membrane is capacitively coupled to a superconducting microwave cavity. Integration with the microwave cavity enables capacitive readout of the mechanical resonance directly on the chip. A microwave pump field and a second probe field are simultaneously applied to the cavity. The accreted mass landing on the membrane can be measured conveniently by tracking the mechanical resonance frequency shifts due to mass changes in the probe transmission spectrum. The mass responsivity for the membrane is 0.72 Hz/ag and we demonstrate that frequency shifts induced by adsorption of one hundred 1587 bp DNA molecules can be well resolved in the probe transmission spectrum.

Electron beam physical vapor deposition of thin ruby films for remote temperature sensing

NASA Astrophysics Data System (ADS)

Li, Wei; Coppens, Zachary J.; Greg Walker, D.; Valentine, Jason G.

2013-04-01

Thermographic phosphors (TGPs) possessing temperature-dependent photoluminescence properties have a wide range of uses in thermometry due to their remote access and large temperature sensitivity range. However, in most cases, phosphors are synthesized in powder form, which prevents their use in high resolution micro and nanoscale thermal microscopy. In the present study, we investigate the use of electron beam physical vapor deposition to fabricate thin films of chromium-doped aluminum oxide (Cr-Al2O3, ruby) thermographic phosphors. Although as-deposited films were amorphous and exhibited weak photoluminescence, the films regained the stoichiometry and α-Al2O3 crystal structure of the combustion synthesized source powder after thermal annealing. As a consequence, the annealed films exhibit both strong photoluminescence and a temperature-dependent lifetime that decreases from 2.9 ms at 298 K to 2.1 ms at 370 K. Ruby films were also deposited on multiple substrates. To ensure a continuous film with smooth surface morphology and strong photoluminescence, we use a sapphire substrate, which is thermal expansion coefficient and lattice matched to the film. These thin ruby films can potentially be used as remote temperature sensors for probing the local temperatures of micro and nanoscale structures.

Development of a brain monitoring system for multimodality investigation in awake rats.

PubMed

Limnuson, Kanokwan; Narayan, Raj K; Chiluwal, Amrit; Bouton, Chad; Ping Wang; Chunyan Li

2016-08-01

Multimodal brain monitoring is an important approach to gain insight into brain function, modulation, and pathology. We have developed a unique micromachined neural probe capable of real-time continuous monitoring of multiple physiological, biochemical and electrophysiological variables. However, to date, it has only been used in anesthetized animals due to a lack of an appropriate interface for awake animals. We have developed a versatile headstage for recording the small neural signal and bridging the sensors to the remote sensing units for multimodal brain monitoring in awake rats. The developed system has been successfully validated in awake rats by simultaneously measuring four cerebral variables: electrocorticography, oxygen tension, temperature and cerebral blood flow. Reliable signal recordings were obtained with minimal artifacts from movement and environmental noise. For the first time, multiple variables of cerebral function and metabolism were simultaneously recorded from awake rats using a single neural probe. The system is envisioned for studying the effects of pharmacologic treatments, mapping the development of central nervous system diseases, and better understanding normal cerebral physiology.

Silicon Field Effect Transistors as Dual-Use Sensor-Heater Hybrids

PubMed Central

Reddy, Bobby; Elibol, Oguz H.; Nair, Pradeep R.; Dorvel, Brian R.; Butler, Felice; Ahsan, Zahab; Bergstrom, Donald E.; Alam, Muhammad A.; Bashir, Rashid

2011-01-01

We demonstrate the temperature mediated applications of a previously proposed novel localized dielectric heating method on the surface of dual purpose silicon field effect transistor (FET) sensor-heaters and perform modeling and characterization of the underlying mechanisms. The FETs are first shown to operate as electrical sensors via sensitivity to changes in pH in ionic fluids. The same devices are then demonstrated as highly localized heaters via investigation of experimental heating profiles and comparison to simulation results. These results offer further insight into the heating mechanism and help determine the spatial resolution of the technique. Two important biosensor platform applications spanning different temperature ranges are then demonstrated: a localized heat-mediated DNA exchange reaction and a method for dense selective functionalization of probe molecules via the heat catalyzed complete desorption and reattachment of chemical functionalization to the transistor surfaces. Our results show that the use of silicon transistors can be extended beyond electrical switching and field-effect sensing to performing localized temperature controlled chemical reactions on the transistor itself. PMID:21214189

Optical thermometry based on green upconversion emission in Er3+/Yb3+ codoped BaGdF5 glass ceramics

NASA Astrophysics Data System (ADS)

Wu, Ting; Zhao, Shilong; Lei, Ruoshan; Huang, Lihui; Xu, Shiqing

2018-02-01

Er3+/Yb3+ codoped BaGdF5 glass ceramics have been prepared and used to develop a portable all-fiber temperature sensor based on fluorescence intensity ratio technique. XRD and TEM results affirm the generation of BaGdF5 nanocrystals in the borosilicate glass. Eu3+ ions are used as spectral probe to investigate external environment around rare earth (RE) ions. Intense green upconversion emissions from Er3+ ions are detected in the BaGdF5 glass ceramics and their intensity are enhanced about three orders of magnitude after heat treatment, which is attributed to the enrichment of RE ions in the BaGdF5 phase. Based on green upconversion emission from Er3+ ions, the temperature sensing property of the portable all-fiber temperature sensor is studied. The maximum absolute sensitivity is 15.5 × 10-4 K-1 at 567 K and the relative sensitivity is 1.28% K-1 at 298 K, respectively.

The Conformation of Thermoresponsive Polymer Brushes Probed by Optical Reflectivity.

PubMed

Varma, Siddhartha; Bureau, Lionel; Débarre, Delphine

2016-04-05

We describe a microscope-based optical setup that allows us to perform space- and time-resolved measurements of the spectral reflectance of transparent substrates coated with ultrathin films. This technique is applied to investigate the behavior in water of thermosensitive polymer brushes made of poly(N-isopropylacrylamide) grafted on glass. We show that spectral reflectance measurements yield quantitative information about the conformation and axial structure of the brushes as a function of temperature. We study how parameters such as grafting density and chain length affect the hydration state of a brush, and provide one of the few experimental evidences for the occurrence of vertical phase separation in the vicinity of the lower critical solution temperature of the polymer. The origin of the hysteretic behavior of poly(N-isopropylacrylamide) brushes upon cycling the temperature is also clarified. We thus demonstrate that our optical technique allows for in-depth characterization of stimuli-responsive polymer layers, which is crucial for the rational design of smart polymer coatings in actuation, gating, or sensing applications.

Chromenoquinoline-based thiol probes: a study on the quencher position for controlling fluorescent Off-On characteristics.

PubMed

Kand, Dnyaneshwar; Kalle, Arunasree Marasanapalli; Talukdar, Pinaki

2013-02-13

The design, synthesis and thiol sensing ability of chromenoquinoline-based fluorescent probes 4, 5 and 6 and are reported here. The relative position of the maleimide moiety was varied along the chromenoquinoline fluorophore to decrease the background fluorescence. Lower background fluorescence in probes 4 and 6 was rationalized by the smaller k(r)/k(nr) values compared to that of probe 5. An intramolecular charge transfer (ICT) mechanism was proposed for quenching and the extent was dependent on the position of the maleimide quencher. Fluorescent Off-On characteristics were evaluated by theoretical calculations. All probes were selective only towards thiol containing amino acids. Thiol sensing by probes 4 and 6 were much better compared to 5. Probe 4 displayed a better fluorescence response for less hindered thiol (185-, 223- and 156-fold for Hcy, Cys and GSH, respectively), while for probe 6, a higher enhancement in fluorescence was observed with more hindered thiols (180-, 205- and 245-fold for Hcy, Cys and GSH, respectively). The better response to bulkier thiol, GSH by probe 6 was attributed to the steric crowding at the C-4 position and bulkiness of the GSH group which force the succinimide unit to be in a nearly orthogonal conformation. This spatial arrangement was important in reducing the fluorescence quenching ability of the succinimide moiety. The application of probes 4, 5 and 6 was demonstrated by naked eye detection thiols using a 96-well plate system as well as by live-cell imaging.

Waveguide Calibrator for Multi-Element Probe Calibration

NASA Technical Reports Server (NTRS)

Sommerfeldt, Scott D.; Blotter, Jonathan D.

2007-01-01

A calibrator, referred to as the spider design, can be used to calibrate probes incorporating multiple acoustic sensing elements. The application is an acoustic energy density probe, although the calibrator can be used for other types of acoustic probes. The calibrator relies on the use of acoustic waveguide technology to produce the same acoustic field at each of the sensing elements. As a result, the sensing elements can be separated from each other, but still calibrated through use of the acoustic waveguides. Standard calibration techniques involve placement of an individual microphone into a small cavity with a known, uniform pressure to perform the calibration. If a cavity is manufactured with sufficient size to insert the energy density probe, it has been found that a uniform pressure field can only be created at very low frequencies, due to the size of the probe. The size of the energy density probe prevents one from having the same pressure at each microphone in a cavity, due to the wave effects. The "spider" design probe is effective in calibrating multiple microphones separated from each other. The spider design ensures that the same wave effects exist for each microphone, each with an indivdual sound path. The calibrator s speaker is mounted at one end of a 14-cm-long and 4.1-cm diameter small plane-wave tube. This length was chosen so that the first evanescent cross mode of the plane-wave tube would be attenuated by about 90 dB, thus leaving just the plane wave at the termination plane of the tube. The tube terminates with a small, acrylic plate with five holes placed symmetrically about the axis of the speaker. Four ports are included for the four microphones on the probe. The fifth port is included for the pre-calibrated reference microphone. The ports in the acrylic plate are in turn connected to the probe sensing elements via flexible PVC tubes. These five tubes are the same length, so the acoustic wave effects are the same in each tube. The flexible nature of the tubes allows them to be positioned so that each tube terminates at one of the microphones of the energy density probe, which is mounted in the acrylic structure, or the calibrated reference microphone. Tests performed verify that the pressure did not vary due to bends in the tubes. The results of these tests indicate that the average sound pressure level in the tubes varied by only 0.03 dB as the tubes were bent to various angles. The current calibrator design is effective up to a frequency of approximately 4.5 kHz. This upper design frequency is largely due to the diameter of the plane-wave tubes.

Magnetic induction spectroscopy (MIS)-probe design for cervical tissue measurements.

PubMed

Wang, Jau-Yi; Healey, Timothy; Barker, Anthony; Brown, Brian; Monk, Chris; Anumba, Dilly

2017-05-01

Gradiometers have the advantage of increasing measuring sensitivity, which is particularly useful in magnetic induction spectroscopy (MIS) for bio-impedance measurements. Traditional gradiometers use a pair of field sensing coils equally distant and on opposite sides of a drive coil, which provides high immunity to interference. In this paper, a ferrite-cored coaxial gradiometer probe of 29 mm diameter has been developed for measuring the impedance spectra of cervical tissues in vivo. It consists of a ferrite rod with outer ferrite confinement screening in order to eliminate the signals from surrounding tissue. The magnetic screening efficiency was compared with an air-cored gradiometer probe. For both gradiometer probes, a drive coil and two sensing coils were wound on a borosilicate glass former aligned coaxially with two sensing coils equidistant from the drive coil. The signal sensitivity of those two MIS gradiometers has been measured using saline samples with a conductivity range between 0.1 and 1.1 S m -1 . Finite element methods using COMSOL Multiphysics have been used to simulate the distribution of sensitivity to conductivity over the face of each probe and with depth. The ferrite-cored probe has a sensitivity confined to the volume defined by the gap between the ferrite core and outer tube of ferrite while the air-cored probe without any magnetic shielding had a wide sensitivity over the face and the side of the probe. Four saline samples and one of distilled water with conductivities from 0.1 to 1.1 S m -1 have been used to make conductivity measurements at frequencies of 50 kHz, 100 kHz, and 300 kHz. The measurement accuracy of the air-cored MIS probe was 0.09 S m -1 at 50 kHz, improving to 0.05 S m -1 at 300 kHz. For the ferrite-cored MIS probe, the measurement accuracy was 0.28 S m -1 at 50 kHz, improving to 0.04 S m -1 at 300 kHz. In vivo measurements on human hand have been performed using both types of gradiometers and the conductivity is consistent with reported data.

Correction analysis for a supersonic water cooled total temperature probe tested to 1370 K

NASA Technical Reports Server (NTRS)

Lagen, Nicholas T.; Seiner, John M.

1991-01-01

The authors address the thermal analysis of a water cooled supersonic total temperature probe tested in a Mach 2 flow, up to 1366 K total temperature. The goal of this experiment was the determination of high-temperature supersonic jet mean flow temperatures. An 8.99 cm exit diameter water cooled nozzle was used in the tests. It was designed for exit Mach 2 at 1366 K exit total temperature. Data along the jet centerline were obtained for total temperatures of 755 K, 1089 K, and 1366 K. The data from the total temperature probe were affected by the water coolant. The probe was tested through a range of temperatures between 755 K and 1366 K with and without the cooling system turned on. The results were used to develop a relationship between the indicated thermocouple bead temperature and the freestream total temperature. The analysis and calculated temperatures are presented.

Refractory clad transient internal probe for magnetic field measurements in high temperature plasmas

NASA Astrophysics Data System (ADS)

Kim, Hyundae; Cellamare, Vincent; Jarboe, Thomas R.; Mattick, Arthur T.

2005-05-01

The transient internal probe (TIP) is a diagnostic for local internal field measurements in high temperature plasmas. A verdet material, which rotates the polarization angle of the laser light under magnetic fields, is launched into a plasma at about 1.8km/s. A linearly polarized Ar+ laser illuminates the probe in transit and the light retroreflected from the probe is analyzed to determine the local magnetic field profiles. The TIP has been used for magnetic field measurements on the helicity injected torus where electron temperature Te⩽80eV. In order to apply the TIP in higher temperature plasmas, refractory clad probes have been developed utilizing a sapphire tube, rear disc, and a MgO window on the front. The high melting points of these refractory materials should allow probe operation at plasma electron temperatures up to Te˜300eV. A retroreflecting probe has also been developed using "catseye" optics. The front window is replaced with a plano-convex MgO lens, and the back surface of the probe is aluminized. This approach reduces spurious polarization effects and provides refractory cladding for the probe entrance face. In-flight measurements of a static magnetic field demonstrate the ability of the clad probes to withstand gun-launch acceleration, and provide high accuracy measurements of magnetic field.

Highly Sensitive and Selective Sensing of Free Bilirubin Using Metal-Organic Frameworks-Based Energy Transfer Process.

PubMed

Du, Yaran; Li, Xiqian; Lv, Xueju; Jia, Qiong

2017-09-13

Free bilirubin, a key biomarker for jaundice, was detected with a newly designed fluorescent postsynthetically modified metal organic framework (MOF) (UIO-66-PSM) sensor. UiO-66-PSM was prepared based on the aldimine condensation reaction of UiO-66-NH 2 with 2,3,4-trihydroxybenzaldehyde. The fluorescence of UIO-66-PSM could be effectively quenched by free bilirubin via a fluorescent resonant energy transfer process, thus achieving its recognition of free bilirubin. It was the first attempt to design a MOF-based fluorescent probe for sensing free bilirubin. The probe exhibited fast response time, low detection limit, wide linear range, and high selectivity toward free bilirubin. The sensing system enabled the monitor of free bilirubin in real human serum. Hence, the reported free bilirubin sensing platform has potential applications for clinical diagnosis of jaundice.

Temperature sensing using a Cr:ZnGa2O4 new phosphor

NASA Astrophysics Data System (ADS)

Sharma, S. K.; Glais, E.; Pellerin, M.; Chaneac, C.; Viana, B.

2016-02-01

The luminescence emission of a thermographic phosphor based on trivalent chromium doped ZGO (ZnGa2O4) bulk as well as nanoparticles is here reported. This material has a strong temperature dependence on the optical features such as ratio of their emission bands, bandwidths, bands position as well as the lifetime decay of the Cr3+. This makes this material well suitable as temperature sensor. ZnGa2O4 (ZGO), a normal spinel, exhibits a high brightness persistent luminescence, when doped with Cr3+ ions and shows an emission spectrum centered at 695 nm. At the nanometric scale, ZGO is used for in vivo imaging with a better signal to background ratio than classical fluorescent NIR probes. In this work we investigate the ability of the host to be a new thermographic phosphor. Several optical features are investigated in a broad temperature range (10 K-700 K). A comparison between bulk material and nanoparticles is introduced. The obtained results could be used to determine the optimal design parameters for sensor development.

Oxidative Unzipping and Transformation of High Aspect Ratio Boron Nitride Nanotubes into “White Graphene Oxide” Platelets

PubMed Central

Nautiyal, Pranjal; Loganathan, Archana; Agrawal, Richa; Boesl, Benjamin; Wang, Chunlei; Agarwal, Arvind

2016-01-01

Morphological and chemical transformations in boron nitride nanotubes under high temperature atmospheric conditions is probed in this study. We report atmospheric oxygen induced cleavage of boron nitride nanotubes at temperatures exceeding 750 °C for the first time. Unzipping is then followed by coalescence of these densely clustered multiple uncurled ribbons to form stacks of 2D sheets. FTIR and EDS analysis suggest these 2D platelets to be Boron Nitride Oxide platelets, with analogous structure to Graphene Oxide, and therefore we term them as “White Graphene Oxide” (WGO). However, not all BNNTs deteriorate even at temperatures as high as 1000 °C. This leads to the formation of a hybrid nanomaterial system comprising of 1D BN nanotubes and 2D BN oxide platelets, potentially having advanced high temperature sensing, radiation shielding, mechanical strengthening, electron emission and thermal management applications due to synergistic improvement of multi-plane transport and mechanical properties. This is the first report on transformation of BNNT bundles to a continuous array of White Graphene Oxide nanoplatelet stacks. PMID:27388704

Oxidative Unzipping and Transformation of High Aspect Ratio Boron Nitride Nanotubes into “White Graphene Oxide” Platelets

NASA Astrophysics Data System (ADS)

Nautiyal, Pranjal; Loganathan, Archana; Agrawal, Richa; Boesl, Benjamin; Wang, Chunlei; Agarwal, Arvind

2016-07-01

Morphological and chemical transformations in boron nitride nanotubes under high temperature atmospheric conditions is probed in this study. We report atmospheric oxygen induced cleavage of boron nitride nanotubes at temperatures exceeding 750 °C for the first time. Unzipping is then followed by coalescence of these densely clustered multiple uncurled ribbons to form stacks of 2D sheets. FTIR and EDS analysis suggest these 2D platelets to be Boron Nitride Oxide platelets, with analogous structure to Graphene Oxide, and therefore we term them as “White Graphene Oxide” (WGO). However, not all BNNTs deteriorate even at temperatures as high as 1000 °C. This leads to the formation of a hybrid nanomaterial system comprising of 1D BN nanotubes and 2D BN oxide platelets, potentially having advanced high temperature sensing, radiation shielding, mechanical strengthening, electron emission and thermal management applications due to synergistic improvement of multi-plane transport and mechanical properties. This is the first report on transformation of BNNT bundles to a continuous array of White Graphene Oxide nanoplatelet stacks.

Intercomparison of Microphysical Properties Derived from In-Situ Cloud and Remote Sensing Probes over the South East Atlantic Ocean

NASA Astrophysics Data System (ADS)

Miller, R.; McFarquhar, G. M.; Gupta, S.; Poellot, M.; O'Brien, J.; Delene, D. J.

2017-12-01

During the Observations of Aerosols Above Clouds and their Interactions (ORACLES) field campaigns, the NASA P3-Orion was equipped with in-situ probes measuring aerosol and cloud microphysical properties, while the NASA ER-2 was equipped with remote sensors retrieving cloud and aerosol quantities. During ORACLES 2017, the P-3 aircraft was equipped with two Clouds Droplet Probes (CDPs) sizing droplets with diameters (D) between 2 and 50µm. The two CDPs were mounted on pylons with different designs, the CDP on the newly designed left wing pylon positioned further below and ahead of the wing, whereas that on the right wing pylon directly below the wing. The P-3 was also equipped with a Cloud and Aerosol Spectrometer (CAS) sizing droplets with 0.51 µm < D < 50 µm, and three optical array probes: a 2D-stero probe (2DS) for 10 µm < D < 1280 µm; a High Volume Precipitation Sampler (HVPS-3), for 150 µm < D < 1.92 cm; and a Cloud Imaging Probe (CIP) for 25 µm < D < 1600 µm. In addition, a Phase Doppler Interferometer (PDI) for 0.5 µm < D < 2500 µm was included. In this presentation, the number distribution functions n(D) derived from different probes in their overlap ranges and bulk quantities, such as liquid water content (LWC), effective radius (re), total number concentration, extinction, skewness, dispersion and kurtosis derived by different probes over equivalent size ranges are compared. Additional comparison with bulk parameters (e.g., LWC measured by King and hot wire probes) and remotely sensed values are also made. The effect of the software package used to process the data is also examined by using two different packages, the National Center for Atmospheric Research Software for OAP Data Analysis (SODA2), and the University of Oklahoma/Illinois' Processing Software (UIOOPS) to process the optical array probe data. These intercomparisons, as a function of aircraft parameters and environmental conditions, help quantify uncertainties in measurements, improve our understanding of conditions under which the probes best function, assist in the development of a probe-independent best estimate of cloud microphysical parameters, and evaluate the quality of remote sensing retrievals. This in turn, will allow the use of these data sets to quantify cloud-aerosol relationships in the southeast Atlantic.

Plasma density perturbation caused by probes at low gas pressure

NASA Astrophysics Data System (ADS)

Sternberg, Natalia; Godyak, Valery

2017-09-01

An analysis of plasma parameter perturbations caused by a spherical probe immersed into a spherical plasma is presented for arbitrary collisionality and arbitrary ratios of probe to plasma dimensions. The plasma was modeled by the fluid plasma equations with ion inertia and nonlinear ion friction force that dominate plasma transport at low gas pressures. Significant depletion of the plasma density around the probe surface has been found. The area of plasma depletion coincides with the sensing area of different kinds of magnetic and microwave probes and will therefore lead to errors in data inferred from measurements with such probes.

Long-wavelength analyte-sensitive luminescent probes and optical (bio)sensors

PubMed Central

Staudinger, Christoph; Borisov, Sergey M

2016-01-01

Long-wavelength luminescent probes and sensors become increasingly popular. They offer the advantage of lower levels of autofluorescence in most biological probes. Due to high penetration depth and low scattering of red and NIR light such probes potentially enable in vivo measurements in tissues and some of them have already reached a high level of reliability required for such applications. This review focuses on the recent progress in development and application of long-wavelength analyte-sensitive probes which can operate both reversibly and irreversibly. Photophysical properties, sensing mechanisms, advantages and limitations of individual probes are discussed. PMID:27134748

10-fold detection range increase in quadrant-photodiode position sensing for photonic force microscope

NASA Astrophysics Data System (ADS)

Perrone, Sandro; Volpe, Giovanni; Petrov, Dmitri

2008-10-01

We propose a technique that permits one to increase by one order of magnitude the detection range of position sensing for the photonic force microscope with quadrant photodetectors (QPDs). This technique takes advantage of the unavoidable cross-talk between output signals of the QPD and does not assume that the output signals are linear in the probe displacement. We demonstrate the increase in the detection range from 150 to 1400 nm for a trapped polystyrene sphere with radius of 300 nm as probe.

10-fold detection range increase in quadrant-photodiode position sensing for photonic force microscope

DOE Office of Scientific and Technical Information (OSTI.GOV)

Perrone, Sandro; Volpe, Giovanni; Petrov, Dmitri

2008-10-15

We propose a technique that permits one to increase by one order of magnitude the detection range of position sensing for the photonic force microscope with quadrant photodetectors (QPDs). This technique takes advantage of the unavoidable cross-talk between output signals of the QPD and does not assume that the output signals are linear in the probe displacement. We demonstrate the increase in the detection range from 150 to 1400 nm for a trapped polystyrene sphere with radius of 300 nm as probe.

10-fold detection range increase in quadrant-photodiode position sensing for photonic force microscope.

PubMed

Perrone, Sandro; Volpe, Giovanni; Petrov, Dmitri

2008-10-01

We propose a technique that permits one to increase by one order of magnitude the detection range of position sensing for the photonic force microscope with quadrant photodetectors (QPDs). This technique takes advantage of the unavoidable cross-talk between output signals of the QPD and does not assume that the output signals are linear in the probe displacement. We demonstrate the increase in the detection range from 150 to 1400 nm for a trapped polystyrene sphere with radius of 300 nm as probe.

Adjustable Pitot Probe

NASA Technical Reports Server (NTRS)

Ashby, George C., Jr.; Robbins, W. Eugene; Horsley, Lewis A.

1991-01-01

Probe readily positionable in core of uniform flow in hypersonic wind tunnel. Formed of pair of mating cylindrical housings: transducer housing and pitot-tube housing. Pitot tube supported by adjustable wedge fairing attached to top of pitot-tube housing with semicircular foot. Probe adjusted both radially and circumferentially. In addition, pressure-sensing transducer cooled internally by water or other cooling fluid passing through annulus of cooling system.

Energy Donor Effect on the Sensing Performance for a Series of FRET-Based Two-Photon Fluorescent Hg2+ Probes

PubMed Central

Zhang, Yujin; Hu, Wei

2017-01-01

Nonlinear optical properties of a series of newly-synthesized molecular fluorescent probes for Hg2+ containing the same acceptor (rhodamine group) are analyzed by using time-dependent density functional theory in combination with analytical response theory. Special emphasis is placed on evolution of the probes’ optical properties in the absence and presence of Hg2+. These compounds show drastic changes in their photoabsorption and photoemission properties when they react with Hg2+, indicating that they are excellent candidates for ratiometric and colorimetric fluorescent chemosensors. Most importantly, the energy donor moiety is found to play a dominant role in sensing performance of these probes. Two-photon absorption cross sections of the compounds are increased with the presence of Hg2+, which theoretically suggests the possibility of the probes to be two-photon fluorescent Hg2+ sensors. Moreover, analysis of molecular orbitals is presented to explore responsive mechanism of the probes, where the fluorescence resonant energy transfer process is theoretically demonstrated. Our results elucidate the available experimental measurements. This work provides guidance for designing efficient two-photon fluorescent probes that are geared towards biological and chemical applications. PMID:28772466

An indentation depth-force sensing wheeled probe for abnormality identification during minimally invasive surgery.

PubMed

Liu, H; Puangmali, P; Zbyszewski, D; Elhage, O; Dasgupta, P; Dai, J S; Seneviratne, L; Althoefer, K

2010-01-01

This paper presents a novel wheeled probe for the purpose of aiding a surgeon in soft tissue abnormality identification during minimally invasive surgery (MIS), compensating the loss of haptic feedback commonly associated with MIS. Initially, a prototype for validating the concept was developed. The wheeled probe consists of an indentation depth sensor employing an optic fibre sensing scheme and a force/torque sensor. The two sensors work in unison, allowing the wheeled probe to measure the tool-tissue interaction force and the rolling indentation depth concurrently. The indentation depth sensor was developed and initially tested on a homogenous silicone phantom representing a good model for a soft tissue organ; the results show that the sensor can accurately measure the indentation depths occurring while performing rolling indentation, and has good repeatability. To validate the ability of the wheeled probe to identify abnormalities located in the tissue, the device was tested on a silicone phantom containing embedded hard nodules. The experimental data demonstrate that recording the tissue reaction force as well as rolling indentation depth signals during rolling indentation, the wheeled probe can rapidly identify the distribution of tissue stiffness and cause the embedded hard nodules to be accurately located.

Remote sensing of soil moisture using airborne hyperspectral data

USGS Publications Warehouse

Finn, M.; Lewis, M.; Bosch, D.; Giraldo, Mario; Yamamoto, K.; Sullivan, D.; Kincaid, R.; Luna, R.; Allam, G.; Kvien, Craig; Williams, M.

2011-01-01

Landscape assessment of soil moisture is critical to understanding the hydrological cycle at the regional scale and in broad-scale studies of biophysical processes affected by global climate changes in temperature and precipitation. Traditional efforts to measure soil moisture have been principally restricted to in situ measurements, so remote sensing techniques are often employed. Hyperspectral sensors with finer spatial resolution and narrow band widths may offer an alternative to traditional multispectral analysis of soil moisture, particularly in landscapes with high spatial heterogeneity. This preliminary research evaluates the ability of remotely sensed hyperspectral data to quantify soil moisture for the Little River Experimental Watershed (LREW), Georgia. An airborne hyperspectral instrument with a short-wavelength infrared (SWIR) sensor was flown in 2005 and 2007 and the results were correlated to in situ soil moisture values. A significant statistical correlation (R2 value above 0.7 for both sampling dates) for the hyperspectral instrument data and the soil moisture probe data at 5.08 cm (2 inches) was determined. While models for the 20.32 cm (8 inches) and 30.48 cm (12 inches) depths were tested, they were not able to estimate soil moisture to the same degree.

Remote sensing of soil moisture using airborne hyperspectral data

USGS Publications Warehouse

Finn, Michael P.; Lewis, Mark (David); Bosch, David D.; Giraldo, Mario; Yamamoto, Kristina H.; Sullivan, Dana G.; Kincaid, Russell; Luna, Ronaldo; Allam, Gopala Krishna; Kvien, Craig; Williams, Michael S.

2011-01-01

Landscape assessment of soil moisture is critical to understanding the hydrological cycle at the regional scale and in broad-scale studies of biophysical processes affected by global climate changes in temperature and precipitation. Traditional efforts to measure soil moisture have been principally restricted to in situ measurements, so remote sensing techniques are often employed. Hyperspectral sensors with finer spatial resolution and narrow band widths may offer an alternative to traditional multispectral analysis of soil moisture, particularly in landscapes with high spatial heterogeneity. This preliminary research evaluates the ability of remotely sensed hyperspectral data to quantify soil moisture for the Little River Experimental Watershed (LREW), Georgia. An airborne hyperspectral instrument with a short-wavelength infrared (SWIR) sensor was flown in 2005 and 2007 and the results were correlated to in situ soil moisture values. A significant statistical correlation (R 2 value above 0.7 for both sampling dates) for the hyperspectral instrument data and the soil moisture probe data at 5.08 cm (2 inches) was determined. While models for the 20.32 cm (8 inches) and 30.48 cm (12 inches) depths were tested, they were not able to estimate soil moisture to the same degree.

Magnetic porous carbon nanocomposites derived from metal-organic frameworks as a sensing platform for DNA fluorescent detection.

PubMed

Tan, Hongliang; Tang, Gonge; Wang, Zhixiong; Li, Qian; Gao, Jie; Wu, Shimeng

2016-10-12

Metal-organic frameworks (MOFs) have emerged as very fascinating functional materials due to their tunable nature and diverse applications. In this work, we prepared a magnetic porous carbon (MPC) nanocomposite by employing iron-containing MOFs (MIL-88A) as precursors through a one-pot thermolysis method. It was found that the MPC can absorb selectively single-stranded DNA (ssDNA) probe to form MPC/ssDNA complex and subsequently quench the labelled fluorescent dye of the ssDNA probe, which is resulted from the synergetic effect of magnetic nanoparticles and carbon matrix. Upon the addition of complementary target DNA, however, the absorbed ssDNA probe could be released from MPC surface by forming double-stranded DNA with target DNA, and accompanied by the recovery of the fluorescence of ssDNA probe. Based on these findings, a sensing platform with low background signal for DNA fluorescent detection was developed. The proposed sensing platform exhibits high sensitivity with detection limit of 1 nM and excellent selectivity to specific target DNA, even single-base mismatched nucleotide can be distinguished. We envision that the presented study would provide a new perspective on the potential applications of MOF-derived nanocomposites in biomedical fields. Copyright © 2016 Elsevier B.V. All rights reserved.

Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe.

PubMed

Jin, Qiang; Feng, Lei; Zhang, Shui-Jun; Wang, Dan-Dan; Wang, Fang-Jun; Zhang, Yi; Cui, Jing-Nan; Guo, Wen-Zhi; Ge, Guang-Bo; Yang, Ling

2017-09-19

In this study, a novel fluorescent detection system for biological sensing of human albumin (HA) was developed on the basis of the pseudoesterase activity and substrate preference of HA. The designed near-infrared (NIR) fluorescent probe (DDAP) could be effectively hydrolyzed by HA, accompanied by significant changes in both color and fluorescence spectrum. The sensing mechanism was fully investigated by fluorescence spectroscopy, NMR, and mass spectra. DDAP exhibited excellent selectivity and sensitivity toward HA over a variety of human plasma proteins, hydrolases, and abundant biomolecules found in human body. The probe has been successfully applied to measure native HA in diluted plasma samples and the secreted HA in the hepatocyte culture supernatant. DDAP has also been used for fluorescence imaging of HA reabsorption in living renal cells, and the results show that the probe exhibits good cell permeability, low cytotoxicity and high imaging resolution. Furthermore, DDAP has been successfully used for real-time tracking the uptaking and degradation of albumin in ex vivo mouse kidney models for the first time. All these results clearly demonstrated that DDAP-based assay held great promise for real-time sensing and tracking HA in complex biological systems, which would be very useful for basic researches and clinical diagnosis of HA-associated diseases.

Development and calibration of a compact self-sensing atomic force microscope head for micro-nano characterization

NASA Astrophysics Data System (ADS)

Guo, Tong; Wang, Siming; Zhao, Jian; Chen, Jinping; Fu, Xing; Hu, Xiaotang

2011-12-01

A compact self-sensing atomic force microscope (AFM) head is developed for the micro-nano dimensional measurement. This AFM head works in tapping mode equipped with a commercial self-sensing probe. This kind of probe can benefit not only from the tuning fork's stable resonant frequency and high quality factor but also from the silicon cantilever's reasonable spring constant. The head is convenient to operate by its simplicity of structure, since it does not need any optical detector to measure the bending of the cantilever. The compact structure makes the head ease to combine with other measuring methods. According to the probe"s characteristics, a method is proposed to quickly calculate the cantilever"s resonance amplitude through measuring its electro-mechanical coupling factor. An experiment system is established based on the nano-measuring machine (NMM) as a high precision positioning stage. Using this system, the approach/retract test is carried out for calibrating the head. The tests can be traced to the meter definition by interferometers in NMM. Experimental results show that the non-linearity error of this AFM head is smaller than 1%, the sensitivity reaches 0.47nm/mV and the measurement stroke is several hundreds of nanometers.

System for Controlling the Stirring Pin of a Friction Stir Welding Apparatus

NASA Technical Reports Server (NTRS)

Ding, R. Jeffrey (Inventor); Romine, Peter L. (Inventor); Oelgoetz, Peter A. (Inventor)

2002-01-01

A control is provided for a friction stir welding apparatus comprising a pin tool which includes a shoulder and a rotating pin extending outwardly from the shoulder of the pin tool and which, in use, is plunged into a workpiece formed contacting workpiece members to stir weld the members together. The control system controls the penetration of the pin tool into the workpiece members which are mounted on a support anvil. The control system includes a pin length controller for controlling pin length relative to the shoulder and for producing a corresponding pin length signal. A pin force sensor senses the force being exerted on the pin during welding and produces a corresponding actual pin force signal. A probe controller controls a probe extending outwardly from the pin, senses a parameter related to the distance between the probe and the supporting anvil and produces a corresponding probe signal. A workpiece standoff sensor senses the standoff distance between the workpiece and the standoff sensor and produces a corresponding standoff signal. A control unit receives the various signals, together with a weld schedule, and, based on these signals and the weld schedule, controls the pin length controller so as to control pin penetration into the workpiece.

Biomedical sensing and display concept improves brain wave monitoring

NASA Technical Reports Server (NTRS)

Trent, R. L.

1970-01-01

Concept for increasing effectiveness of biomedical sensing and display promises greater monitoring capability while lessening high skill requirements in operating personnel. New concept overcomes deficiencies of current system by employing increased number of probes and microelectronic preamplifiers.

Carbon nanotubes as in vivo bacterial probes.

PubMed

Bardhan, Neelkanth M; Ghosh, Debadyuti; Belcher, Angela M

2014-09-17

With the rise in antibiotic-resistant infections, non-invasive sensing of infectious diseases is increasingly important. Optical imaging, although safer and simpler, is less developed than other modalities such as radioimaging, due to low availability of target-specific molecular probes. Here we report carbon nanotubes (SWNTs) as bacterial probes for fluorescence imaging of pathogenic infections. We demonstrate that SWNTs functionalized using M13 bacteriophage (M13-SWNT) can distinguish between F'-positive and F'-negative bacterial strains. Moreover, through one-step modification, we attach an anti-bacterial antibody on M13-SWNT, making it easily tunable for sensing specific F'-negative bacteria. We illustrate detection of Staphylococcus aureus intramuscular infections, with ~3.4 × enhancement in fluorescence intensity over background. SWNT imaging presents lower signal spread ~0.08 × and higher signal amplification ~1.4 × , compared with conventional dyes. We show the probe offers greater ~5.7 × enhancement in imaging of S. aureus infective endocarditis. These biologically functionalized, aqueous-dispersed, actively targeted, modularly tunable SWNT probes offer new avenues for exploration of deeply buried infections.

Carbon nanotubes as in vivo bacterial probes

NASA Astrophysics Data System (ADS)

Bardhan, Neelkanth M.; Ghosh, Debadyuti; Belcher, Angela M.

2014-09-01

With the rise in antibiotic-resistant infections, non-invasive sensing of infectious diseases is increasingly important. Optical imaging, although safer and simpler, is less developed than other modalities such as radioimaging, due to low availability of target-specific molecular probes. Here we report carbon nanotubes (SWNTs) as bacterial probes for fluorescence imaging of pathogenic infections. We demonstrate that SWNTs functionalized using M13 bacteriophage (M13-SWNT) can distinguish between F‧-positive and F‧-negative bacterial strains. Moreover, through one-step modification, we attach an anti-bacterial antibody on M13-SWNT, making it easily tunable for sensing specific F‧-negative bacteria. We illustrate detection of Staphylococcus aureus intramuscular infections, with ~3.4 × enhancement in fluorescence intensity over background. SWNT imaging presents lower signal spread ~0.08 × and higher signal amplification ~1.4 × , compared with conventional dyes. We show the probe offers greater ~5.7 × enhancement in imaging of S. aureus infective endocarditis. These biologically functionalized, aqueous-dispersed, actively targeted, modularly tunable SWNT probes offer new avenues for exploration of deeply buried infections.

Carbon Nanotubes as in vivo Bacterial Probes

PubMed Central

Bardhan, Neelkanth M.; Ghosh, Debadyuti; Belcher, Angela M.

2014-01-01

With the rise in antibiotic-resistant infections, noninvasive sensing of infectious diseases is increasingly important. Optical imaging, while safer and simpler, is less developed than other modalities like radioimaging; due to low availability of target-specific molecular probes. Here, we report carbon nanotubes (SWNTs) as bacterial probes for fluorescence imaging of pathogenic infections. We demonstrate that SWNTs functionalized using M13 bacteriophage (M13-SWNT) can distinguish between F'-positive and F'-negative bacterial strains. Moreover, through one-step modification, we attach an anti-bacterial antibody on M13-SWNT, making it easily tunable for sensing specific F’-negative bacteria. We illustrate detection of Staphylococcus aureus intramuscular infections, with ~3.4× enhancement in fluorescence intensity over background. SWNT imaging presents lower signal spread ~0.08×, and higher signal amplification ~1.4×, compared to conventional dyes. We show the probe offers greater ~5.7× enhancement in imaging of S. aureus infective endocarditis. These biologically-functionalized, aqueous-dispersed, actively-targeted, modularly-tunable SWNT probes offer new avenues for exploration of deeply-buried infections. PMID:25230005

Selective functionalization of carbon nanotube tips allowing fabrication of new classes of nanoscale sensing and manipulation tools

NASA Technical Reports Server (NTRS)

Wade, Lawrence A. (Inventor); Shapiro, Ian R. (Inventor); Bittner, Jr., Vern Garrett (Inventor); Collier, Charles Patrick (Inventor); Esplandiu, Maria J. (Inventor); Giapis, Konstantinos P. (Inventor)

2009-01-01

Embodiments in accordance with the present invention relate to techniques for the growth and attachment of single wall carbon nanotubes (SWNT), facilitating their use as robust and well-characterized tools for AFM imaging and other applications. In accordance with one embodiment, SWNTs attached to an AFM tip can function as a structural scaffold for nanoscale device fabrication on a scanning probe. Such a probe can trigger, with nanometer precision, specific biochemical reactions or conformational changes in biological systems. The consequences of such triggering can be observed in real time by single-molecule fluorescence, electrical, and/or AFM sensing. Specific embodiments in accordance with the present invention utilize sensing and manipulation of individual molecules with carbon nanotubes, coupled with single-molecule fluorescence imaging, to allow observation of spectroscopic signals in response to mechanically induced molecular changes. Biological macromolecules such as proteins or DNA can be attached to nanotubes to create highly specific single-molecule probes for investigations of intermolecular dynamics, for assembling hybrid biological and nanoscale materials, or for developing molecular electronics. In one example, electrical wiring of single redox enzymes to carbon nanotube scanning probes allows observation and electrochemical control over single enzymatic reactions by monitoring fluorescence from a redox-active cofactor or the formation of fluorescent products. Enzymes ''nanowired'' to the tips of carbon nanotubes in accordance with embodiments of the present invention, may enable extremely sensitive probing of biological stimulus-response with high spatial resolution, including product-induced signal transduction.

Design and Application of New Low-Cost Instruments for Marine Environmental Research

PubMed Central

Marcelli, Marco; Piermattei, Viviana; Madonia, Alice; Mainardi, Umberto

2014-01-01

The development of low-cost instrumentation plays a key role in marine environmental studies and represents one of the most innovative aspects of current oceanographic research. These kinds of devices can be used for several applications, ranging from vertical profilers to stand-alone systems, and can be installed on different platforms (buoys, Voluntary Observing Ships, underwater vehicles, etc.). The availability of low-cost technologies enables the realization of extended observatory networks for the study of marine physical and biological processes through an integrated approach merging in situ observations, forecasting models and remotely sensed data. We present new low-cost sensors and probes developed to measure marine temperature, conductivity, chlorophyll a and Chromophoric Dissolved Organic Matter fluorescence, focusing on sensing strategies, general architecture, laboratory trials, in situ tests and comparison with standard instruments. Furthermore, we report the expendable (New T-FLaP), vertical profiler (T-FLaPpro) and stand-alone (Spectra) applications of these technological developments that were tested during several oceanographic surveys in the Mediterranean Sea. PMID:25490594

Design and application of new low-cost instruments for marine environmental research.

PubMed

Marcelli, Marco; Piermattei, Viviana; Madonia, Alice; Mainardi, Umberto

2014-12-05

The development of low-cost instrumentation plays a key role in marine environmental studies and represents one of the most innovative aspects of current oceanographic research. These kinds of devices can be used for several applications, ranging from vertical profilers to stand-alone systems, and can be installed on different platforms (buoys, Voluntary Observing Ships, underwater vehicles, etc.). The availability of low-cost technologies enables the realization of extended observatory networks for the study of marine physical and biological processes through an integrated approach merging in situ observations, forecasting models and remotely sensed data. We present new low-cost sensors and probes developed to measure marine temperature, conductivity, chlorophyll a and Chromophoric Dissolved Organic Matter fluorescence, focusing on sensing strategies, general architecture, laboratory trials, in situ tests and comparison with standard instruments. Furthermore, we report the expendable (New T-FLaP), vertical profiler (T-FLaPpro) and stand-alone (Spectra) applications of these technological developments that were tested during several oceanographic surveys in the Mediterranean Sea.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Graham, Eric A.; Patange, Omkar; Lukac, Martin

With improved cookstoves (ICs) increasingly distributed to households for a range of air pollution interventions and carbon-credit programs, it has become necessary to accurately monitor the duration of cooking and the amount of fuel consumed. In this study, laboratory trials were used to create temperature-based algorithms for quantifying cooking duration and estimating fuel consumption from stove temperatures. Field validation of the algorithms employed a Wireless Cookstove Sensing System (WiCS) that offers remote, low-cost temperature sensing and the wireless transmission of temperature data to a centralized database using local cellular networks. Field trials included 68 unscripted household cooking events. In themore » laboratory, temperature responses of the IC body and that of a removable temperature probe (J-bar) followed well-known physical models during cooking, indicating that location of the temperature sensor is not critical. In the laboratory, the classification correctly identified active cooking 97.2% of the time. In the field, the cooking duration was not statistically different from that recorded by trained volunteers; the average difference between calculated and observed cooking times was 0.03 ± 0.31 h (mean ± SD). In the laboratory, energy flux from the IC was calculated using temperatures measured by the J-bar and on the IC body and found to be proportional to the total energy in the consumed fuel, with an r 2 correlation value of 0.95. Here in the field, the average fuel consumption was calculated to be 0.97 ± 0.32 kg compared to that recorded by volunteers of 1.19 ± 0.37 kg with an average difference between calculated and observed fuel mass of 0.21 ± 0.37 kg per event. Finally, despite wide variation in observed cooking duration and fuel consumption per event, a relatively constant rate of fuel consumption of 0.48 kg h -1 was calculated for users of the same type of IC.« less

Characteristics of the Fiber Laser Sensor System Based on Etched-Bragg Grating Sensing Probe for Determination of the Low Nitrate Concentration in Water.

PubMed

Pham, Thanh Binh; Bui, Huy; Le, Huu Thang; Pham, Van Hoi

2016-12-22

The necessity of environmental protection has stimulated the development of many kinds of methods allowing the determination of different pollutants in the natural environment, including methods for determining nitrate in source water. In this paper, the characteristics of an etched fiber Bragg grating (e-FBG) sensing probe-which integrated in fiber laser structure-are studied by numerical simulation and experiment. The proposed sensor is demonstrated for determination of the low nitrate concentration in a water environment. Experimental results show that this sensor could determine nitrate in water samples at a low concentration range of 0-80 ppm with good repeatability, rapid response, and average sensitivity of 3.5 × 10 -3 nm/ppm with the detection limit of 3 ppm. The e-FBG sensing probe integrated in fiber laser demonstrates many advantages, such as a high resolution for wavelength shift identification, high optical signal-to-noise ratio (OSNR of 40 dB), narrow bandwidth of 0.02 nm that enhanced accuracy and precision of wavelength peak measurement, and capability for optical remote sensing. The obtained results suggested that the proposed e-FBG sensor has a large potential for the determination of low nitrate concentrations in water in outdoor field work.

Note: Production of stable colloidal probes for high-temperature atomic force microscopy applications

NASA Astrophysics Data System (ADS)

Ditscherlein, L.; Peuker, U. A.

2017-04-01

For the application of colloidal probe atomic force microscopy at high temperatures (>500 K), stable colloidal probe cantilevers are essential. In this study, two new methods for gluing alumina particles onto temperature stable cantilevers are presented and compared with an existing method for borosilicate particles at elevated temperatures as well as with cp-cantilevers prepared with epoxy resin at room temperature. The durability of the fixing of the particle is quantified with a test method applying high shear forces. The force is calculated with a mechanical model considering both the bending as well as the torsion on the colloidal probe.

First measurements of electron temperature in the D region with a symmetric double probe

NASA Technical Reports Server (NTRS)

Szuszczewicz, E. P.

1973-01-01

Measurement of the altitude profile of electron temperature in the ionospheric D region with the aid of a symmetric double probe flown on a Nike-Cajun payload launched on Oct. 13, 1971. The procedure for determining the electron temperature from the parameters of the double probe's current-voltage characteristic under conditions of nonnegligible ion-atom collision frequencies is described. It is shown that in its first lower ionospheric application the technique of the symmetric double probe has yielded the lowest values of electron temperature yet measured and has provided the very first direct measurement of electron temperature in the D region.

Proceedings of the STRESS Data Review Meeting, 29-30 November 1977

DTIC Science & Technology

1978-06-01

INSULATORS MAGNETOMETER BEACON ANTENNA fe?^ S-BAND ANTENNA- -DC PROBE SENSING ELEMENT PLASMA FREQUENCY PROBE MONOPOLE -GUARD ELECTRODE PLASMA...have demonstrated, using calculational results from MICE and MRHYDE (MHD computer codes), that the gradient-drift instability is the one primarily

Development of an optical fiber SERS microprobe for minimally invasive sensing applications

NASA Astrophysics Data System (ADS)

Mamun, Md Abdullah Al; Juodkazis, Saulius; Mahadevan-Jansen, Anita; Stoddart, Paul R.

2018-02-01

Numerous potential biomedical sensing applications of surface-enhanced Raman scattering (SERS) have been reported, but its practical use has been limited by the lack of a robust sensing platform. Optical fiber SERS probes show great promise, but are limited by the prominent silica Raman background, which requires the use of bulky optics for filtering the signal collection and excitation delivery paths. In the present study, a SERS microprobe has been designed and developed to eliminate the bottlenecks outlined above. For efficient excitation and delivery of the SERS signal, both hollow core photonic crystal fiber and double clad fiber have been investigated. While the hollow core fiber was still found to have excessive silica background, the double clad fiber allows efficient signal collection via the multi-mode inner cladding. A micro filtering mechanism has been designed, which can be integrated into the tip of the optical fiber SERS probe, providing filtering to suppress silica Raman background and thus avoiding the need for bulky optics. The design also assists in the efficient collection of SERS signal from the sample by rejecting Rayleigh scattered light from the sample. Optical fiber cleaving using ultra-short laser pulses was tested for improved control of the fiber tip geometry. With this miniaturized and integrated filtering mechanism, it is expected that the developed probe will promote the use of SERS for minimally invasive biomedical monitoring and sensing applications in future. The probe could potentially be placed inside a small gauge hypodermic needle and would be compatible with handheld portable spectrometers.

Double positive effect of adding hexaethyelene glycol when optimizing the hybridization efficiency of a microring DNA detection assay

NASA Astrophysics Data System (ADS)

Van Eeghem, Anabelle; Werquin, Sam; Hoste, Jan-Willem; Goes, Arne; Vanderleyden, Els; Bienstman, Peter; Dubruel, Peter

2017-05-01

In this paper, a method for detection of DNA molecules using silicon-on-insulator (SOI) microring resonators is described. The influence of temperature and the use of formamide on the hybridization efficiency were studied. It was shown that 50 v/v% of formamide in the hybridization buffer can ensure hybridization when working close to physiological temperature. Furthermore, the use of hexaethylene glycol (HEG) as backfilling agent was studied in order to resolve issues of non-specific adsorption to the surface. The results indicated that not only non-specific binding was reduced significantly but also that HEG improves the orientation of the DNA probes on the surface. This led to a 4-fold increase in hybridization efficiency and thus in an equal decrease in the detection limit, compared to hybridization without the use of HEG. An improvement in robustness of the assay was also observed. This DNA reorientation hypothesis was confirmed by studying the thickness and density of the layers by using dual polarization microring sensing. Finally, the different steps in the sensing experiment were characterized in more detail by static contact angle (SCA) and X-ray photoelectron spectroscopy (XPS) analysis. The results showed quantitatively that the surface modifications were successful.

Attomolar detection of proteins via cascade strand-displacement amplification and polystyrene nanoparticle enhancement in fluorescence polarization aptasensors.

PubMed

Huang, Yong; Liu, Xiaoqian; Huang, Huakui; Qin, Jian; Zhang, Liangliang; Zhao, Shulin; Chen, Zhen-Feng; Liang, Hong

2015-08-18

Extremely sensitive and accurate measurements of protein markers for early detection and monitoring of diseases pose a formidable challenge. Herein, we develop a new type of amplified fluorescence polarization (FP) aptasensor based on allostery-triggered cascade strand-displacement amplification (CSDA) and polystyrene nanoparticle (PS NP) enhancement for ultrasensitive detection of proteins. The assay system consists of a fluorescent dye-labeled aptamer hairpin probe and a PS NP-modified DNA duplex (assistant DNA/trigger DNA duplex) probe with a single-stranded part and DNA polymerase. Two probes coexist stably in the absence of target, and the dye exhibits relatively low FP background. Upon recognition and binding with a target protein, the stem of the aptamer hairpin probe is opened, after which the opened hairpin probe hybridizes with the single-stranded part in the PS NP-modified DNA duplex probe and triggers the CSDA reaction through the polymerase-catalyzed recycling of both target protein and trigger DNA. Throughout this CSDA process, numerous massive dyes are assembled onto PS NPs, which results in a substantial FP increase that provides a readout signal for the amplified sensing process. Our newly proposed amplified FP aptasensor enables the quantitative measurement of proteins with the detection limit in attomolar range, which is about 6 orders of magnitude lower than that of traditional homogeneous aptasensors. Moreover, this sensing method also exhibits high specificity for target proteins and can be performed in homogeneous solutions. In addition, the suitability of this method for the quantification of target protein in biological samples has also been shown. Considering these distinct advantages, the proposed sensing method can be expected to provide an ultrasensitive platform for the analysis of various types of target molecules.

121. Man with temperature probe aimed at armature measuring temperature ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

121. Man with temperature probe aimed at armature measuring temperature as armature heats up between the two electrodes. March 27, 1985 - Statue of Liberty, Liberty Island, Manhattan, New York County, NY

Novel design of microgyroscopes employing electrostatic actuation and resistance-change based sensing

NASA Astrophysics Data System (ADS)

Ghommem, M.; Abdelkefi, A.

2017-12-01

The nonlinear dynamics of a microgyroscope consisting of a vibrating beam with attached proof mass and operating at high frequency is numerically investigated. The working principle of this inertial sensor is based on exploiting the transfer of the mechanical energy among two vibrations modes via the Coriolis effect to measure the rotation rate. The flexural motion (drive mode) is generated by applying a DC electrostatic load and an AC harmonic load. We propose a novel sensing technique based on resistance change to detect the induced vibrations of the microbeam (sense mode) and extract the rotation rate. The sensing technique is based on transmitting the Coriolis force acting on the proof mass to a probe that affects the resistance of an electrical circuit acting as a variable voltage divider. This is achieved by integrating the probe dipping μpool (PDP) technology deploying a probe electrode that is dipped into a μpool filled with a conductive nonvolatile fluid. Large magnitude of the AC harmonic load is observed to give rise to dynamic pull-in bandwidth in the frequency response characterized by large and uncontrollable vibrations of the microbeam. Operating near the primary frequency while selecting moderate AC voltage results in linear calibration curves while maintaining high sensitivity of the output voltage to the change in the rotation speed. The simulation results demonstrate the feasibility of the novel technique for sensing the induced vibrations to deliver measurements of the angular speed.

High-Temperature Piezoelectric Sensing

PubMed Central

Jiang, Xiaoning; Kim, Kyungrim; Zhang, Shujun; Johnson, Joseph; Salazar, Giovanni

2014-01-01

Piezoelectric sensing is of increasing interest for high-temperature applications in aerospace, automotive, power plants and material processing due to its low cost, compact sensor size and simple signal conditioning, in comparison with other high-temperature sensing techniques. This paper presented an overview of high-temperature piezoelectric sensing techniques. Firstly, different types of high-temperature piezoelectric single crystals, electrode materials, and their pros and cons are discussed. Secondly, recent work on high-temperature piezoelectric sensors including accelerometer, surface acoustic wave sensor, ultrasound transducer, acoustic emission sensor, gas sensor, and pressure sensor for temperatures up to 1,250 °C were reviewed. Finally, discussions of existing challenges and future work for high-temperature piezoelectric sensing are presented. PMID:24361928

Combined experimental and theoretical studies on selective sensing of zinc and pyrophosphate ions by rational design of compartmental chemosensor probe: Dual sensing behaviour via secondary recognition approach and cell imaging studies.

PubMed

Mawai, Kiran; Nathani, Sandip; Roy, Partha; Singh, U P; Ghosh, Kaushik

2018-05-08

A compartmental chemosensor probe HL has been designed and synthesized for the selective recognition of zinc ions over other transition metal ions via fluorescence "ON" strategy. The chemosensing behaviour of HL was demonstrated through fluorescence, absorption and NMR spectroscopic techniques. The molecular structure of the zinc complex derived from HL was determined by X-ray crystallography. A probable mechanism of this selective sensing behavior was described on the basis of spectroscopic results and theoretical studies by density functional theory (DFT). The biological applicability of the chemosensor HL was examined via cell imaging on HeLa cells. The HL-zinc complex served as a secondary fluorescent probe responding to the pyrophosphate anion specifically over other anions. The fluorescence enhancement of HL in association with Zn2+ ions was quenched in the presence of pyrophosphate (PPi). Thus, a dual response was established based on "OFF-ON-OFF" strategy for detection of both cation and anion. This phenomenon was utilized in the construction of a "INHIBIT" logic gate.

Fiber-optic temperature probe system for inner body

NASA Astrophysics Data System (ADS)

Liu, Bo; Deng, Xing-Zhong; Cao, Wei; Cheng, Xianping; Xie, Tuqiang; Zhong, Zugen

1991-08-01

The authors have designed a fiber-optic temperature probe system that can quickly insert its probe into bodies to measure temperature. Its thermometer unit has the function of program- controlled zeroing. The single-chip microcomputer is used to control the whole system and process data. The sample system has been tested in a coal furnace.

High performance SERS on nanoporous gold substrates synthesized by chemical de-alloying a Au-based metallic glass

NASA Astrophysics Data System (ADS)

Xue, Yanpeng; Scaglione, Federico; Rizzi, Paola; Battezzati, Livio

2017-12-01

A Au20Cu48Ag7Pd5Si20 metallic glass precursor has been used to synthesize nanoporous gold by chemical de-alloying in a mixture of HNO3 and HF. Gold ligaments of size ranging from 45 to 100 nm were obtained as a function of HNO3 concentration, electrolyte temperature and de-alloying time. The as-prepared nanoporous gold exhibited strong surface enhanced Raman scattering (SERS) effect using 4,4‧-bi-pyridine as probe molecule. For application in melamine sensing, the detection limit of 10-6 M was achieved, which indicated that this biocompatible material has great potential as SERS active substrate.

Ultra-bright emission from hexagonal boron nitride defects as a new platform for bio-imaging and bio-labelling

NASA Astrophysics Data System (ADS)

Elbadawi, Christopher; Tran, Trong Toan; Shimoni, Olga; Totonjian, Daniel; Lobo, Charlene J.; Grosso, Gabriele; Moon, Hyowan; Englund, Dirk R.; Ford, Michael J.; Aharonovich, Igor; Toth, Milos

2016-12-01

Bio-imaging requires robust ultra-bright probes without causing any toxicity to the cellular environment, maintain their stability and are chemically inert. In this work we present hexagonal boron nitride (hBN) nanoflakes which exhibit narrowband ultra-bright single photon emitters1. The emitters are optically stable at room temperature and under ambient environment. hBN has also been noted to be noncytotoxic and seen significant advances in functionalization with biomolecules2,3. We further demonstrate two methods of engineering this new range of extremely robust multicolour emitters across the visible and near infrared spectral ranges for large scale sensing and biolabeling applications.

Miniature Cavity-Enhanced Diamond Magnetometer

NASA Astrophysics Data System (ADS)

Chatzidrosos, Georgios; Wickenbrock, Arne; Bougas, Lykourgos; Leefer, Nathan; Wu, Teng; Jensen, Kasper; Dumeige, Yannick; Budker, Dmitry

2017-10-01

We present a highly sensitive miniaturized cavity-enhanced room-temperature magnetic-field sensor based on nitrogen-vacancy centers in diamond. The magnetic resonance signal is detected by probing absorption on the 1042-nm spin-singlet transition. To improve the absorptive signal the diamond is placed in an optical resonator. The device has a magnetic-field sensitivity of 28 pT /√{Hz } , a projected photon shot-noise-limited sensitivity of 22 pT /√{Hz } , and an estimated quantum projection-noise-limited sensitivity of 0.43 pT /√{Hz } with the sensing volume of ˜390 μ m ×4500 μ m2 . The presented miniaturized device is the basis for an endoscopic magnetic-field sensor for biomedical applications.

Quantum sensing with arbitrary frequency resolution

NASA Astrophysics Data System (ADS)

Boss, J. M.; Cujia, K. S.; Zopes, J.; Degen, C. L.

2017-05-01

Quantum sensing takes advantage of well-controlled quantum systems for performing measurements with high sensitivity and precision. We have implemented a concept for quantum sensing with arbitrary frequency resolution, independent of the qubit probe and limited only by the stability of an external synchronization clock. Our concept makes use of quantum lock-in detection to continuously probe a signal of interest. Using the electronic spin of a single nitrogen-vacancy center in diamond, we demonstrate detection of oscillating magnetic fields with a frequency resolution of 70 microhertz over a megahertz bandwidth. The continuous sampling further guarantees an enhanced sensitivity, reaching a signal-to-noise ratio in excess of 104 for a 170-nanotesla test signal measured during a 1-hour interval. Our technique has applications in magnetic resonance spectroscopy, quantum simulation, and sensitive signal detection.

Analysis of a Pneumatic Probe for Measuring Exhaust-Gas Temperatures with Some Preliminary Experimental Results

NASA Technical Reports Server (NTRS)

Scadron, Marvin D.

1952-01-01

A pneumatic probe based on continuity of mass flow through two restrictions separated by a cooling chamber was constructed to measure gas temperature at and beyond the limit of thermocouples. This probe consisted of a subsonic flat-plate orifice for the first restriction and a sonic-flow converging-diverging nozzle for the second restriction. The effect of variation in gas constants on the calibration is examined for common engine-exhaust gases. A high-temperature wind tunnel that allowed calibration of the probe at temperatures up to 2000 deg R and. Mach numbers up to 0.8 is described. Agreement to better than 30 deg R between pneumatic probe indication and the indication of a rake of radiation shielded thermocouples indicates that extrapolation of the calibration to higher temperatures is possible with fair accuracy

Sense of Community in Student Retention at a Tertiary Technical Institution in Bogotá: An Ecological Approach

ERIC Educational Resources Information Center

Mendoza, Pilar; Suarez, Juan Diego; Bustamante, Eileen

2016-01-01

Objective: This study probes the reasons for high attrition rates and sense of community among students enrolled at a technical institution serving low-income students in Bogotá, Colombia. Although sense of community on campus is the strongest predictor of a student's thriving, scholars in higher education have studied mainly minority students'…

Three axis velocity probe system

DOEpatents

Fasching, George E.; Smith, Jr., Nelson S.; Utt, Carroll E.

1992-01-01

A three-axis velocity probe system for determining three-axis positional velocities of small particles in fluidized bed systems and similar applications. This system has a sensor head containing four closely-spaced sensing electrodes of small wires that have flat ends to establish a two axis plane, e.g. a X-Y plane. Two of the sensing electrodes are positioned along one of the axes and the other two are along the second axis. These four sensing electrodes are surrounded by a guard electrode, and the outer surface is a ground electrode and support member for the sensing head. The electrodes are excited by, for example, sinusoidal voltage having a peak-to-peak voltage of up to 500 volts at a frequency of 2 MHz. Capacitive currents flowing between the four sensing electrodes and the ground electrode are influenced by the presence and position of a particle passing the sensing head. Any changes in these currents due to the particle are amplified and synchronously detected to produce positional signal values that are converted to digital form. Using these digital forms and two values of time permit generation of values of the three components of the particle vector and thus the total velocity vector.

Validity and reliability of temperature measurement by heat flow thermistors, flexible thermocouple probes and thermistors in a stirred water bath.

PubMed

Versey, Nathan G; Gore, Christopher J; Halson, Shona L; Plowman, Jamie S; Dawson, Brian T

2011-09-01

We determined the validity and reliability of heat flow thermistors, flexible thermocouple probes and general purpose thermistors compared with a calibrated reference thermometer in a stirred water bath. Validity (bias) was defined as the difference between the observed and criterion values, and reliability as the repeatability (standard deviation or typical error) of measurement. Data were logged every 5 s for 10 min at water temperatures of 14, 26 and 38 °C for ten heat flow thermistors and 24 general purpose thermistors, and at 35, 38 and 41 °C for eight flexible thermocouple probes. Statistical analyses were conducted using spreadsheets for validity and reliability, where an acceptable bias was set at ±0.1 °C. None of the heat flow thermistors, 17% of the flexible thermocouple probes and 71% of the general purpose thermistors met the validity criterion for temperature. The inter-probe reliabilities were 0.03 °C for heat flow thermistors, 0.04 °C for flexible thermocouple probes and 0.09 °C for general purpose thermistors. The within trial intra-probe reliability of all three temperature probes was 0.01 °C. The results suggest that these temperature sensors should be calibrated individually before use at relevant temperatures and the raw data corrected using individual linear regression equations.

Method and apparatus for optical temperature measurement

DOEpatents

O'Rourke, P.E.; Livingston, R.R.; Prather, W.S.

1994-09-20

A temperature probe and a method for using said probe for temperature measurements based on changes in light absorption by the probe are disclosed. The probe comprises a first and a second optical fiber that carry light to and from the probe, and a temperature sensor material, the absorbance of which changes with temperature, through which the light is directed. Light is directed through the first optical fiber, passes through the temperature sensor material, and is transmitted by a second optical fiber from the material to a detector. Temperature-dependent and temperature-independent factors are derived from measurements of the transmitted light intensity. For each sensor material, the temperature T is a function of the ratio, R, of these factors. The temperature function f(R) is found by applying standard data analysis techniques to plots of T versus R at a series of known temperatures. For a sensor having a known temperature function f(R) and known characteristic and temperature-dependent factors, the temperature can be computed from a measurement of R. Suitable sensor materials include neodymium-doped borosilicate glass, accurate to [+-]0.5 C over an operating temperature range of about [minus]196 C to 400 C; and a mixture of D[sub 2]O and H[sub 2]O, accurate to [+-]0.1 C over an operating range of about 5 C to 90 C. 13 figs.

Method and apparatus for optical temperature measurement

DOEpatents

O'Rourke, Patrick E.; Livingston, Ronald R.; Prather, William S.

1994-01-01

A temperature probe and a method for using said probe for temperature measurements based on changes in light absorption by the probe. The probe comprises a first and a second optical fiber that carry light to and from the probe, and a temperature sensor material, the absorbance of which changes with temperature, through which the light is directed. Light is directed through the first optical fiber, passes through the temperature sensor material, and is transmitted by a second optical fiber from the material to a detector. Temperature-dependent and temperature-independent factors are derived from measurements of the transmitted light intensity. For each sensor material, the temperature T is a function of the ratio, R, of these factors. The temperature function f(R) is found by applying standard data analysis techniques to plots of T versus R at a series of known temperatures. For a sensor having a known temperature function f(R) and known characteristic and temperature-dependent factors, the temperature can be computed from a measurement of R. Suitable sensor materials include neodymium-doped boresilicate glass, accurate to .+-.0.5.degree. C. over an operating temperature range of about -196.degree. C. to 400.degree. C.; and a mixture of D.sub.2 O and H.sub.2 O, accurate to .+-.0.1.degree. C. over an operating range of about 5.degree. C. to 90.degree. C.

Perspectives of methods of laser monitoring of the atmosphere and sea surface

NASA Astrophysics Data System (ADS)

Pashayev, Arif; Tunaboylu, Bahadir; Usta, Metin; Sadixov, Ilham; Allahverdiyev, Kerim

2016-01-01

Laser monitoring (remote sensing) may be considered as the science of collecting and interpreting information about the atmosphere, earth and sea using sensors on earth, on platforms in our atmosphere (airplanes, balloons) or in space (satellites) without being in direct physical contact with them. Remote sensing by LIDARs (Light Identification Detection and Ranging) has wide applications as technique to probe the Earth's atmosphere, ocean and land surfaces. LIDARs are widely used to get knowledge of spatial and temporal variations in meteorological quantities (e.g. temperature, humidity, clouds and aerosol properties) and to monitor the changes in these quantities on different timescales. Subject of the present work is quite wide. It is rather difficult to perform analysis and to provide full knowledge about existing information. In the present work, in addition to the literature data, the information will be provided also about KA-09 aerosol LIDAR developed at the Marmara Research Centre of TÜBITAK (Turkish Scientific and technological Research Council) and also about KA-14 LIDAR developed at the National Aviation Academy of Azerbaijan for remote sensing of contaminations on water surfaces taking place during oil-gas production. The main goal of this paper is to give students insight in different remote sensing instruments and techniques (including their perspectives) that are used for the derivation of meteorological quantities and obtaining the information about water surface.

Millimeter-Wave Propagation and Remote Sensing of the Atmosphere,

DTIC Science & Technology

1983-12-01

tool to probe lower atmospheric structure. The principal applications of millimeter waves have been in the areas of communications, radar, and remote ... sensing . The availability of large bandwidths makes this region of the spectrum particularly attractive for high data rate communications. Because

Clean, Logistically Light Access to Explore the Closest Places on Earth to Europa and Enceladus

NASA Astrophysics Data System (ADS)

Winebrenner, D. P.; Elam, W. T.; Kintner, P. M. S.; Tyler, S.; Selker, J. S.

2016-12-01

At present, the logistical costs of ice drilling to depths of kilometers severely limit sampling and measurements beneath ice sheets. Thus only a tiny fraction of the 400 known subglacial lakes beneath the Antarctic Ice Sheet can ever be sampled by drilling, and study of large lakes may be limited to observations at one or, at best, a few sites. Antarctic lakes are likely highly diverse in their geochemical and geothermal fluxes, the timing and duration of their glaciations, and other characteristics. They constitute a remarkable collection of natural laboratories for learning biogeochemistries and adaptations of subglacial life on Earth. Moreover, they are arguably Earth-analogs to ice-covered seas on Europa and Enceladus, closer not only in relative terms than other analogs, but also usefully close in absolute terms for learning solar-system-wide features of ice-covered seas. It is therefore essential to sample Antarctic lakes with enough range and density, in space and time, to gain better understanding of their workings than drilling alone can provide. The logistics of thermal melt probes makes them attractive, provided that key limitations can be overcome. In particular, melt probes from the 1960s through the 1990s were unreliable, all halted in their descents by electrical failures at high voltages (which are necessary for efficient power use). Moreover, the hole above a classical melt probe refreezes, so neither samples nor the probe itself can be recovered. Here we report progress in overcoming both of these limitations with modern materials and components for reliable high-voltage operation. We have demonstrated in Greenland a 6.5 cm-diameter melt probe operating at 1050V/2.15 kW (electrical) that descended at 2.4 m/hr to 80 m depth in 2013, and after restarting in 2014, to 400 m depth, where we turned it off. We also operated a probe at 2000V/4.5 kW in 2014, which descended at 6.6 m/hr (according to a validated engineering model). These results are the second greatest depth and greatest speed attained by melt probes. We also report on testing a way to avoid complete refreezing of a melt hole, which enables cable deployment from the surface and thus small probes to reach and be recovered from great depth, as well as Raman Distributed Temperature Sensing of ice sheet temperatures, with several applications.

Using linear polarization for LWIR hyperspectral sensing of liquid contaminants

NASA Astrophysics Data System (ADS)

Thériault, Jean-Marc; Fortin, Gilles; Lacasse, Paul; Bouffard, François; Lavoie, Hugo

2013-09-01

We report and analyze recent results obtained with the MoDDIFS sensor (Multi-option Differential Detection and Imaging Fourier Spectrometer) for the passive polarization sensing of liquid contaminants in the long wave infrared (LWIR). Field measurements of polarized spectral radiance done on ethylene glycol and SF96 probed at distances of 6.5 and 450 meters, respectively, have been used to develop and test a GLRT-type detection algorithm adapted for liquid contaminants. The GLRT detection results serve to establish the potential and advantage of probing the vertical and horizontal linear hyperspectral polarization components for improving liquid contaminants detection.

Non-invasive probe diagnostic method for electron temperature and ion current density in atmospheric pressure plasma jet source

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kim, Young-Cheol; Kim, Yu-Sin; Lee, Hyo-Chang

2015-08-15

The electrical probe diagnostics are very hard to be applied to atmospheric plasmas due to severe perturbation by the electrical probes. To overcome this, the probe for measuring electron temperature and ion current density is indirectly contacted with an atmospheric jet source. The plasma parameters are obtained by using floating harmonic analysis. The probe is mounted on the quartz tube that surrounds plasma. When a sinusoidal voltage is applied to a probe contacting on a quartz tube, the electrons near the sheath at dielectric tube are collected and the probe current has harmonic components due to probe sheath nonlinearity. Frommore » the relation of the harmonic currents and amplitude of the sheath voltage, the electron temperature near the wall can be obtained with collisional sheath model. The electron temperatures and ion current densities measured at the discharge region are in the ranges of 2.7–3.4 eV and 1.7–5.2 mA/cm{sup 2} at various flow rates and input powers.« less

High-Temperature RF Probe Station For Device Characterization Through 500 deg C and 50 GHz

NASA Technical Reports Server (NTRS)

Schwartz, Zachary D.; Downey, Alan N.; Alterovitz, Samuel A.; Ponchak, George E.; Williams, W. D. (Technical Monitor)

2003-01-01

A high-temperature measurement system capable of performing on-wafer microwave testing of semiconductor devices has been developed. This high temperature probe station can characterize active and passive devices and circuits at temperatures ranging from room temperature to above 500 C. The heating system uses a ceramic heater mounted on an insulating block of NASA shuttle tile material. The temperature is adjusted by a graphical computer interface and is controlled by the software-based feedback loop. The system is used with a Hewlett-Packard 8510C Network Analyzer to measure scattering parameters over a frequency range of 1 to 50 GHz. The microwave probes, cables, and inspection microscope are all shielded to protect from heat damage. The high temperature probe station has been successfully used to characterize gold transmission lines on silicon carbide at temperatures up to 540 C.

Coordinate metrology using scanning probe microscopes

NASA Astrophysics Data System (ADS)

Marinello, F.; Savio, E.; Bariani, P.; Carmignato, S.

2009-08-01

New positioning, probing and measuring strategies in coordinate metrology are needed for the accomplishment of true three-dimensional characterization of microstructures, with uncertainties in the nanometre range. In the present work, the implementation of scanning probe microscopes (SPMs) as systems for coordinate metrology is discussed. A new non-raster measurement approach is proposed, where the probe is moved to sense points along free paths on the sample surface, with no loss of accuracy with respect to traditional raster scanning and scan time reduction. Furthermore, new probes featuring long tips with innovative geometries suitable for coordinate metrology through SPMs are examined and reported.

A benzothiazole-based fluorescent probe for hypochlorous acid detection and imaging in living cells

NASA Astrophysics Data System (ADS)

Nguyen, Khac Hong; Hao, Yuanqiang; Zeng, Ke; Fan, Shengnan; Li, Fen; Yuan, Suke; Ding, Xuejing; Xu, Maotian; Liu, You-Nian

2018-06-01

A benzothiazole-based turn-on fluorescent probe with a large Stokes shift (190 nm) has been developed for hypochlorous acid detection. The probe displays prompt fluorescence response for HClO with excellent selectivity over other reactive oxygen species as well as a low detection limit of 0.08 μM. The sensing mechanism involves the HClO-induced specific oxidation of oxime moiety of the probe to nitrile oxide, which was confirmed by HPLC-MS technique. Furthermore, imaging studies demonstrated that the probe is cell permeable and can be applied to detect HClO in living cells.

Designing probe from E6 genome region of human Papillomavirus 16 for sensing applications.

PubMed

Parmin, Nor Azizah; Hashim, Uda; Gopinath, Subash C B

2018-02-01

Human Papillomavirus (HPV) is a standout amongst the most commonly reported over 100 types, among them genotypes 16, 18, 31 and 45 are the high-risk HPV. Herein, we designed the oligonucleotide probe for the detection of predominant HPV type 16 for the sensing applications. Conserved amino acid sequences within E6 region of the open reading frame in the HPV genome was used as the basis to design oligonucleotide probe to detect cervical cancer. Analyses of E6 amino acid sequences from the high-risk HPVs were done to check the percentage of similarity and consensus regions that cause different cancers, including cervical cancer. Basic local alignment search tools (BLAST) have given extra statistical parameters, for example, desire values (E-values) and score bits. The probe, 'GGG GTC GGT GGA CCG GTC GAT GTA' was designed with 66.7% GC content. This oligonucleotide probe is designed with the length of 24 mer, GC percent is between 40 and 70, and the melting point (Tm) is above 50°C. The probe needed an acceptable length between 22 and 31 mer. The choice of region is identified here can be used as a probe, has implications for HPV detection techniques in biosensor especially for clinical determination of cervical cancer. Copyright © 2017 Elsevier B.V. All rights reserved.

Thin-film fiber optic hydrogen and temperature sensor system

DOEpatents

Nave, Stanley E.

1998-01-01

The invention discloses a sensor probe device for monitoring of hydrogen gas concentrations and temperatures by the same sensor probe. The sensor probe is constructed using thin-film deposition methods for the placement of a multitude of layers of materials sensitive to hydrogen concentrations and temperature on the end of a light transparent lens located within the sensor probe. The end of the lens within the sensor probe contains a lens containing a layer of hydrogen permeable material which excludes other reactive gases, a layer of reflective metal material that forms a metal hydride upon absorbing hydrogen, and a layer of semi-conducting solid that is transparent above a temperature dependent minimum wavelength for temperature detection. The three layers of materials are located at the distal end of the lens located within the sensor probe. The lens focuses light generated by broad-band light generator and connected by fiber-optics to the sensor probe, onto a reflective metal material layer, which passes through the semi-conducting solid layer, onto two optical fibers located at the base of the sensor probe. The reflected light is transmitted over fiberoptic cables to a spectrometer and system controller. The absence of electrical signals and electrical wires in the sensor probe provides for an elimination of the potential for spark sources when monitoring in hydrogen rich environments, and provides a sensor free from electrical interferences.

Thin-film fiber optic hydrogen and temperature sensor system

DOEpatents

Nave, S.E.

1998-07-21

The invention discloses a sensor probe device for monitoring of hydrogen gas concentrations and temperatures by the same sensor probe. The sensor probe is constructed using thin-film deposition methods for the placement of a multitude of layers of materials sensitive to hydrogen concentrations and temperature on the end of a light transparent lens located within the sensor probe. The end of the lens within the sensor probe contains a lens containing a layer of hydrogen permeable material which excludes other reactive gases, a layer of reflective metal material that forms a metal hydride upon absorbing hydrogen, and a layer of semi-conducting solid that is transparent above a temperature dependent minimum wavelength for temperature detection. The three layers of materials are located at the distal end of the lens located within the sensor probe. The lens focuses light generated by broad-band light generator and connected by fiber-optics to the sensor probe, onto a reflective metal material layer, which passes through the semi-conducting solid layer, onto two optical fibers located at the base of the sensor probe. The reflected light is transmitted over fiber optic cables to a spectrometer and system controller. The absence of electrical signals and electrical wires in the sensor probe provides for an elimination of the potential for spark sources when monitoring in hydrogen rich environments, and provides a sensor free from electrical interferences. 3 figs.

T-28 data acquisition during COHMEX 1986

NASA Technical Reports Server (NTRS)

Musil, Dennis J.; Smith, Paul L.

1986-01-01

As part of the 1986 Cooperative Huntsville Meteorological Experiment (COHMEX) a cloud physics instrumented T-28 aircraft was used in conjunction with multiple ground based Doppler radars to characterize hydrometeors and updraft structure within developing summertime cumulus and cumulonimbus cloud systems near Huntsville, Alabama. Instrumentation aboard the aircraft included a Particle Measuring Systems (PMS) Forward Scattering Spectrometer Probe (FSSP), a PMS 2D Cloud Probe and a PMS 2D Precipitation Probe, as well as a hail spectrometer and a foil impactor. Hydrometeor spectra were obtained in the interior of mature thunderstorms over the size range from cloud droplets through hailstones. In addition, vertical wind speed, temperature, Johnson-Williams (JW) liquid water content and electric field measurements were made. Significant microphysical differences exist between these clouds and summertime cumulonimbus clouds which develop over the Central Plains. One notable difference in clouds displaying similar radar reflectivities is that COHMEX hydrometeors are typically smaller and more numerous than those observed in the Central Plains. The COHMEX cloud microphysical measurements represent ground truth values for the remote sensing instrumentation which was flown over the cloud tops at altitudes between 60,000 and 70,000 ft aboard NASA U-2 and ER-2 aircraft. They are also being used jointly with a numerical cloud model to assist in understanding the development of summertime subtropical clouds.

Acoustic temperature measurement in a rocket noise field.

PubMed

Giraud, Jarom H; Gee, Kent L; Ellsworth, John E

2010-05-01

A 1 μm diameter platinum wire resistance thermometer has been used to measure temperature fluctuations generated during a static GEM-60 rocket motor test. Exact and small-signal relationships between acoustic pressure and acoustic temperature are derived in order to compare the temperature probe output with that of a 3.18 mm diameter condenser microphone. After preliminary plane wave tests yielded good agreement between the transducers within the temperature probe's ∼2 kHz bandwidth, comparison between the temperature probe and microphone data during the motor firing show that the ±∼3 K acoustic temperature fluctuations are a significant contributor to the total temperature variations.

Real-time data acquisition and telemetry based irrigation control system

DOEpatents

Slater, John M.; Svoboda, John M.

2005-12-13

A data acquisition and telemetry based control system for use in facilitating substantially real time management of an agricultural irrigation system. The soil moisture sensor includes a reader and a plurality of probes. The probes each include an electronic circuit having a moisture sensing capacitor in operative communication with the soil whose moisture is to be measured. Each probe also includes a receive/transmit antenna and the reader includes a transmit/receive antenna, so that as the reader passes near the probe, the reader transmits a digital excitation signal to the electronic circuit of the biodegradable probe via an inductive couple formed between the transmit/receive antenna of the reader and the receive/transmit coil of the probe. The electronic circuit uses an energy component of the excitation signal to generate a digital data signal which indicates the moisture content of the soil adjacent to the moisture sensing capacitor. The probe sends the data signal to the reader which then uses the data signal to develop a corresponding set of watering instructions which are then transmitted to a control module in communication with the irrigation system. The control module sends corresponding control signals to nozzles of the irrigation system causing the irrigation system to disperse water in a manner consistent with the moisture content data transmitted by the probes to the reader. Because the irrigation system moves continuously through the field to be irrigated, the moisture content data acquisition and resultant water dispersal by the irrigation system occur substantially in real time.

Aggregation induced emission enhancement (AIEE) characteristics of quinoline based compound - A versatile fluorescent probe for pH, Fe(III) ion, BSA binding and optical cell imaging

NASA Astrophysics Data System (ADS)

Manikandan, Irulappan; Chang, Chien-Huei; Chen, Chia-Ling; Sathish, Veerasamy; Li, Wen-Shan; Malathi, Mahalingam

2017-07-01

Novel benzimidazoquinoline derivative (AVT) was synthesized through a substitution reaction and characterized by various spectral techniques. Analyzing the optical properties of AVT under absorption and emission spectral studies in different environments exclusively with respect to solvents and pH, intriguing characteristics viz. aggregation induced emission enhancement (AIEE) in the THF solvent and 'On-Off' pH sensing were found at neutral pH. Sensing nature of AVT with diverse metal ions and bovine serum albumin (BSA) was also studied. Among the metal ions, Fe3 + ion alone tunes the fluorescence intensity of AVT probe in aqueous medium from ;turn-on; to ;turn-off; through ligand (probe) to metal charge transfer (LMCT) mechanism. The probe AVT in aqueous medium interacts strongly with BSA due to Fluorescence Resonance Energy Transfer (FRET) and the conformational change in BSA was further analyzed using synchronous fluorescence techniques. Docking study of AVT with BSA reveals that the active site of binding is tryptophan residue which is also supported by the experimental results. Interestingly, fluorescent AVT probe in cells was examined through cellular imaging studies using BT-549 and MDA-MB-231 cells. Thus, the single molecule probe based detection of multiple species and stimuli were described.

Intra-opeartive OCT imaging and sensing devices for clinical translation (Conference Presentation)

NASA Astrophysics Data System (ADS)

Chen, Yu

2017-02-01

Stereotactic procedures that require insertion of needle-based instruments into the brain serve important roles in a variety of neurosurgical interventions, such as biopsy, catheterization, and electrode placement. A fundamental limitation of these stereotactic procedures is that they are blind procedures in that the operator does not have real-time feedback as to what lies immediately ahead of the advancing needle. Therefore, there is a great clinical need to navigate the instrument safely and accurately to the targets. Towards that end, we developed a forwarding-imaging needle-type optical coherence tomography (OCT) probe for avoiding the hemorrhage and guiding neurosurgical interventions. The needle probe has a thin diameter of 0.7 mm. The feasibility of vessel detection and neurosurgical guidance were demonstrated on sheep brain in vivo and human brain ex vivo. In addition, we further reduced the probe size to 0.3 mm using an optical Doppler sensing (ODS) fiber probe that can integrate with microelectrode recording (MER) to detect the blood vessels lying ahead to improve the safety of this procedure. Furthermore, to overcome the field-of-view limitation of OCT probe, we developed an MRI-compatible OCT imaging probe for neurosurgery. MRI/OCT multi-scale imaging integrates micro-resolution optical imaging with wide-field MRI imaging, and has potential to further improve the targeting accuracy.

FIRE_AX_UKMO_C130

Atmospheric Science Data Center

2015-11-25

... FSSP Gust Probe Hot-Wire Hygrometer Platinum Resistance PMS 2D-C Probe PRT-4 Pyranometer Pyrgeometer ... Parameters:  Barometric Altitude Cloud Top Temperature Deiced Temperature Dew/Frost Point Temperature Droplet ...

Simultaneous in situ electron temperature comparisons using Alouette 2 probe and plasma resonance data

NASA Technical Reports Server (NTRS)

Benson, R. F.

1973-01-01

The electron temperatures deduced from Alouette 2 diffuse resonance observations are compared with the temperature obtained from the Alouette 2 cylindrical electrostatic probe experiment using data from 5 mid-to-high latitude telemetry stations. The probe temperature is consistently higher than the diffuse resonance temperature. The average difference ranged from approximately 10% to 40% with the lower values occurring at the lowest altitudes sampled (near 500 km) and at high latitudes (dip latitude greater than 55 deg), and the larger values occurring at high altitudes and lower latitudes. The discrepancy appears to be of geophysical origin since it is dependent on the location of the data sample. The present observations support the view that the often observed radar backscatter - probe electron temperature discrepancy is also of geophysical origin.

Linear servomotor probe drive system with real-time self-adaptive position control for the Alcator C-Mod tokamak

NASA Astrophysics Data System (ADS)

Brunner, D.; Kuang, A. Q.; LaBombard, B.; Burke, W.

2017-07-01

A new servomotor drive system has been developed for the horizontal reciprocating probe on the Alcator C-Mod tokamak. Real-time measurements of plasma temperature and density—through use of a mirror Langmuir probe bias system—combined with a commercial linear servomotor and controller enable self-adaptive position control. Probe surface temperature and its rate of change are computed in real time and used to control probe insertion depth. It is found that a universal trigger threshold can be defined in terms of these two parameters; if the probe is triggered to retract when crossing the trigger threshold, it will reach the same ultimate surface temperature, independent of velocity, acceleration, or scrape-off layer heat flux scale length. In addition to controlling the probe motion, the controller is used to monitor and control all aspects of the integrated probe drive system.

Apparatus and method for high temperature viscosity and temperature measurements

DOEpatents

Balasubramaniam, Krishnan; Shah, Vimal; Costley, R. Daniel; Singh, Jagdish P.

2001-01-01

A probe for measuring the viscosity and/or temperature of high temperature liquids, such as molten metals, glass and similar materials comprises a rod which is an acoustical waveguide through which a transducer emits an ultrasonic signal through one end of the probe, and which is reflected from (a) a notch or slit or an interface between two materials of the probe and (b) from the other end of the probe which is in contact with the hot liquid or hot melt, and is detected by the same transducer at the signal emission end. To avoid the harmful effects of introducing a thermally conductive heat sink into the melt, the probe is made of relatively thermally insulative (non-heat-conductive) refractory material. The time between signal emission and reflection, and the amplitude of reflections, are compared against calibration curves to obtain temperature and viscosity values.

Eddy current testing probe with dual half-cylindrical coils

NASA Astrophysics Data System (ADS)

Bae, Byung-Hoon; Choi, Jung-Mi; Kim, Soo-Yong

2000-02-01

We have developed a new eddy current probe composed of a dual half-cylindrical (2HC) coil as an exciting coil and a sensing coil that is placed in the small gap of the 2HC coil. The 2HC coil induces a linear eddy current on the narrow region within the target medium. The magnitude of eddy current has a maximum peak with the narrow width, underneath the center of the exciting 2HC coil. Because of the linear eddy current, the probe can be used to detect not only the existence of a crack but also its direction in conducting materials. Using specimen with a machined crack, and varying the exciting frequency from 0.5 to 100 kHz, we investigated the relationships between the direction of crack and the output voltage of the sensing coil.

Amplifier Module for 260-GHz Band Using Quartz Waveguide Transitions

NASA Technical Reports Server (NTRS)

Padmanabhan, Sharmila; Fung, King Man; Kangaslahti, Pekka P.; Peralta, Alejandro; Soria, Mary M.; Pukala, David M.; Sin, Seth; Samoska, Lorene A.; Sarkozy, Stephen; Lai, Richard

2012-01-01

Packaging of MMIC LNA (monolithic microwave integrated circuit low-noise amplifier) chips at frequencies over 200 GHz has always been problematic due to the high loss in the transition between the MMIC chip and the waveguide medium in which the chip will typically be used. In addition, above 200 GHz, wire-bond inductance between the LNA and the waveguide can severely limit the RF matching and bandwidth of the final waveguide amplifier module. This work resulted in the development of a low-loss quartz waveguide transition that includes a capacitive transmission line between the MMIC and the waveguide probe element. This capacitive transmission line tunes out the wirebond inductance (where the wire-bond is required to bond between the MMIC and the probe element). This inductance can severely limit the RF matching and bandwidth of the final waveguide amplifier module. The amplifier module consists of a quartz E-plane waveguide probe transition, a short capacitive tuning element, a short wire-bond to the MMIC, and the MMIC LNA. The output structure is similar, with a short wire-bond at the output of the MMIC, a quartz E-plane waveguide probe transition, and the output waveguide. The quartz probe element is made of 3-mil quartz, which is the thinnest commercially available material. The waveguide band used is WR4, from 170 to 260 GHz. This new transition and block design is an improvement over prior art because it provides for better RF matching, and will likely yield lower loss and better noise figure. The development of high-performance, low-noise amplifiers in the 180-to- 700-GHz range has applications for future earth science and planetary instruments with low power and volume, and astrophysics array instruments for molecular spectroscopy. This frequency band, while suitable for homeland security and commercial applications (such as millimeter-wave imaging, hidden weapons detection, crowd scanning, airport security, and communications), also has applications to future NASA missions. The Global Atmospheric Composition Mission (GACM) in the NRC Decadel Survey will need low-noise amplifiers with extremely low noise temperatures, either at room temperature or for cryogenic applications, for atmospheric remote sensing.

Multiplexed fibre optic sensing in the distal lung (Conference Presentation)

NASA Astrophysics Data System (ADS)

Choudhary, Tushar R.; Tanner, Michael G.; Megia-Fernandez, Alicia; Harrington, Kerrianne; Wood, Harry A.; Chankeshwara, Sunay; Zhu, Patricia; Choudhury, Debaditya; Yu, Fei; Thomson, Robert R.; Duncan, Rory R.; Dhaliwal, Kevin; Bradley, Mark

2017-02-01

We present a toolkit for a multiplexed pH and oxygen sensing probe in the distal lung using multicore fibres. Measuring physiological relevant parameters like pH and oxygen is of significant importance in understanding changes associated with disease pathology. We present here, a single multicore fibre based pH and oxygen sensing probe which can be used with a standard bronchoscope to perform in vivo measurements in the distal lung. The multiplexed probe consists of fluorescent pH sensors (fluorescein based) and oxygen sensors (Palladium porphyrin complex based) covalently bonded to silica microspheres (10 µm) loaded on the distal facet of a 19 core (10 µm core diameter) multicore fibre (total diameter of 150 µm excluding coating). Pits are formed by selectively etching the cores using hydrofluoric acid, multiplexing is achieved through the self-location of individual probes on differing cores. This architecture can be expanded to include probes for further parameters. Robust measurements are demonstrated of self-referencing fluorophores, not limited by photobleaching, with short (100ms) measurement times at low ( 10µW) illumination powers. We have performed on bench calibration and tests of in vitro tissue models and in an ovine whole lung model to validate our sensors. The pH sensor is demonstrated in the physiologically relevant range of pH 5 to pH 8.5 and with an accuracy of ± 0.05 pH units. The oxygen sensor is demonstrated in gas mixtures downwards from 20% oxygen and in liquid saturated with 20% oxygen mixtures ( 8mg/L) down to full depletion (0mg/L) with 0.5mg/L accuracy.

Self-sensing of dielectric elastomer actuator enhanced by artificial neural network

NASA Astrophysics Data System (ADS)

Ye, Zhihang; Chen, Zheng

2017-09-01

Dielectric elastomer (DE) is a type of soft actuating material, the shape of which can be changed under electrical voltage stimuli. DE materials have promising usage in future’s soft actuators and sensors, such as soft robotics, energy harvesters, and wearable sensors. In this paper, a stripe DE actuator with integrated sensing capability is designed, fabricated, and characterized. Since the strip actuator can be approximated as a compliant capacitor, it is possible to detect the actuator’s displacement by analyzing the actuator’s impedance change. An integrated sensing scheme that adds a high frequency probing signal into actuation signal is developed. Electrical impedance changes in the probing signal are extracted by fast Fourier transform algorithm, and nonlinear data fitting methods involving artificial neural network are implemented to detect the actuator’s displacement. A series of experiments show that by improving data processing and analyzing methods, the integrated sensing method can achieve error level of lower than 1%.

Acoustical experiment of yogurt fermentation process.

PubMed

Ogasawara, H; Mizutani, K; Ohbuchi, T; Nakamura, T

2006-12-22

One of the important factors through food manufacturing is hygienic management. Thus, food manufactures prove their hygienic activities by taking certifications like a Hazard Analysis and Critical Control Point (HACCP). This concept also applies to food monitoring. Acoustical measurements have advantage for other measurement in food monitoring because they make it possible to measure with noncontact and nondestructive. We tried to monitor lactic fermentation of yogurt by a probing sensor using a pair of acoustic transducers. Temperature of the solution changes by the reaction heat of fermentation. Consequently the sound velocity propagated through the solution also changes depending on the temperature. At the same time, the solution change its phase from liquid to gel. The transducers usage in the solution indicates the change of the temperature as the change of the phase difference between two transducers. The acoustic method has advantages of nondestructive measurement that reduces contamination of food product by measuring instrument. The sensor was inserted into milk with lactic acid bacterial stain of 19 degrees C and monitored phase retardation of propagated acoustic wave and its temperature with thermocouples in the mild. The monitoring result of fermentation from milk to Caspian Sea yogurt by the acoustic transducers with the frequency of 3.7 MHz started to show gradient change in temperature caused by reaction heat of fermentation but stop the gradient change at the end although the temperature still change. The gradient change stopped its change because of phase change from liquid to gel. The present method will be able to measure indirectly by setting transducers outside of the measuring object. This noncontact sensing method will have great advantage of reduces risk of food contamination from measuring instrument because the measurement probes are set out of fermentation reactor or food containers. Our proposed method will contribute to the hygienization for the food manufacture industry.

Nanoparticle-based luminescent probes for intracellular sensing and imaging of pH.

PubMed

Schäferling, Michael

2016-05-01

Fluorescence imaging microscopy is an essential tool in biomedical research. Meanwhile, various fluorescent probes are available for the staining of cells, cell membranes, and organelles. Though, to monitor intracellular processes and dysfunctions, probes that respond to ubiquitous chemical parameters determining the cellular function such as pH, pO2 , and Ca(2+) are required. This review is focused on the progress in the design, fabrication, and application of photoluminescent nanoprobes for sensing and imaging of pH in living cells. The advantages of using nanoprobes carrying fluorescent pH indicators compared to single molecule probes are discussed as well as their limitations due to the mostly lysosomal uptake by cells. Particular attention is paid to ratiometric dual wavelength nanosensors that enable intrinsic referenced measurements. Referencing and proper calibration procedures are basic prerequisites to carry out reliable quantitative pH determinations in complex samples such as living cells. A variety of examples will be presented that highlight the diverseness of nanocarrier materials (polymers, micelles, silica, quantum dots, carbon dots, gold, photon upconversion nanocrystals, or bacteriophages), fluorescent pH indicators for the weak acidic range, and referenced sensing mechanisms, that have been applied intracellularly up to now. WIREs Nanomed Nanobiotechnol 2016, 8:378-413. doi: 10.1002/wnan.1366 For further resources related to this article, please visit the WIREs website. © 2015 Wiley Periodicals, Inc.

C-QDs@UiO-66-(COOH)2 Composite Film via Electrophoretic Deposition for Temperature Sensing.

PubMed

Feng, Ji-Fei; Gao, Shui-Ying; Shi, Jianlin; Liu, Tian-Fu; Cao, Rong

2018-03-05

Temperature plays a crucial role in both scientific research and industry. However, traditional temperature sensors, such as liquid-filled thermometers, thermocouples, and transistors, require contact to obtain heat equilibrium between the probe and the samples during the measurement. In addition, traditional temperature sensors have limitations when being used to detect the temperature change of fast-moving samples at smaller scales. Herein, the carbon quantum dots (C-QDs) functionalized metal-organic framework (MOF) composite film, a novel contactless solid optical thermometer, has been prepared via electrophoretic deposition (EPD). Instead of terephthalic acid (H 2 BDC), 1',2',4',5'-benzenetetracarboxylic (H 4 BTEC) acid was employed to construct a UiO-66 framework to present two uncoordinated carboxylic groups decorated on the pore surface. The uncoordinated carboxylic groups can generate negative charges, which facilitates the deposition of film on the positive electrode during the EPD process. Moreover, UiO-66-(COOH) 2 MOFs can absorb C-QDs from the solution and prevent C-QDs from aggregating, and the well-dispersed C-QDs impart fluorescence characteristics to composites. As-synthesized composite film was successfully used to detect temperature change in the range of 97-297 K with a relative sensitivity up to 1.3% K -1 at 297 K.

Sensing temperature via downshifting emissions of lanthanide-doped metal oxides and salts. A review

NASA Astrophysics Data System (ADS)

Dramićanin, Miroslav D.

2016-12-01

Temperature is important because it has an effect on even the tiniest elements of daily life and is involved in a broad spectrum of human activities. That is why it is the most commonly measured physical quantity. Traditional temperature measurements encounter difficulties when used in some emerging technologies and environments, such as nanotechnology and biomedicine. The problem may be alleviated using optical techniques, one of which is luminescence thermometry. This paper reviews the state of luminescence thermometry and presents different temperature read-out schemes with an emphasis on those utilizing the downshifting emission of lanthanide-doped metal oxides and salts. The read-out schemes for temperature include those based on measurements of spectral characteristics of luminescence (band positions and shapes, emission intensity and ratio of emission intensities), and those based on measurements of the temporal behavior of luminescence (lifetimes and rise times). This review (with 140 references) gives the basics of the fundamental principles and theory that underlie the methods presented, and describes the methodology for the estimation of their performance. The major part of the text is devoted to those lanthanide-doped metal oxides and salts that are used as temperature probes, and to the comparison of their performance and characteristics.

A neutron spectrometer based on temperature variations in superheated drop compositions

NASA Astrophysics Data System (ADS)

Apfel, Robert E.; d'Errico, Francesco

2002-01-01

The response of superheated drop detectors (SDDs) to neutron radiation varies in a self-consistent manner with variations in temperature and pressure, making such compositions suitable for neutron spectrometry. The advantage of this approach is that the response functions of candidate materials versus energy as the temperature or pressure is varied are nested and have distinct thresholds, with no thermal neutron response. These characteristics permit unfolding without the uncertainties associated with other spectrometry techniques, where multiple solutions are possible, thus requiring an initial guess of the spectrum. A spectrometer was developed based on the well-established technology for acoustic sensing of bubble events interfaced with a proportional-integral-derivative temperature controller. The active monitor for neutrons, called REMbrandt™, was used as the platform for controlling temperature on a SDD probe and for data acquisition, thereby automating the process of measuring the neutron energy spectrum. The new instrument, called REM-SPEC™, implements and automates the original BINS approach: it adjusts the temperature of the SDD vial in increasing steps and measures the bubble event rate at each step. By using two distinct SDD materials with overlapping responses, the 0.1-20 MeV range of energies relevant to practical spectrometry is readily covered. Initial experiments with an Am-Be source validate the operational protocols of this device.

Final Report fir DE-SC0005507 (A1618): The Development of an Improved Cloud Microphysical Product for Model and Remote Sensing Evaluation using RACORO Observations

DOE Office of Scientific and Technical Information (OSTI.GOV)

McFarquhar, Greg M.

2012-09-21

We proposed to analyze data collected during the Routine Aerial Facilities (AAF) Clouds with Low Optical Water Depths (CLOWD) Optical Radiative Observations (RACORO) in order to develop an integrated product of cloud microphysical properties (number concentration of drops in different size bins, total liquid drop concentration integrated over all bin sizes, liquid water content LWC, extinction of liquid clouds, effective radius of water drops, and radar reflectivity factor) that could be used to evaluate large-eddy simulations (LES), general circulation models (GCMs) and ground-based remote sensing retrievals, and to develop cloud parameterizations with the end goal of improving the modeling ofmore » cloud processes and properties and their impact on atmospheric radiation. We have completed the development of this microphysical database. We investigated the differences in the size distributions measured by the Cloud and Aerosol Spectrometer (CAS) and the Forward Scattering Probe (FSSP), between the one dimensional cloud imaging probe (1DC) and the two-dimensional cloud imaging probe (2DC), and between the bulk LWCs measured by the Gerber probe against those derived from the size resolved probes.« less

Real time detection of ESKAPE pathogens by a nitroreductase-triggered fluorescence turn-on probe.

PubMed

Xu, Shengnan; Wang, Qinghua; Zhang, Qingyang; Zhang, Leilei; Zuo, Limin; Jiang, Jian-Dong; Hu, Hai-Yu

2017-10-18

The identification of bacterial pathogens is the critical first step in conquering infection diseases. A novel turn-on fluorescent probe for the selective sensing of nitroreductase (NTR) activity and its initial applications in rapid, real-time detection and identification of ESKAPE pathogens have been reported.

Water-soluble mercury ion sensing based on the thymine-Hg2+-thymine base pair using retroreflective Janus particle as an optical signaling probe.

PubMed

Chun, Hyeong Jin; Kim, Saemi; Han, Yong Duk; Kim, Dong Woo; Kim, Ka Ram; Kim, Hyo-Sop; Kim, Jae-Ho; Yoon, Hyun C

2018-05-01

Herein, we report an optical sensing platform for mercury ions (Hg 2+ ) in water based on the integration of Hg 2+ -mediated thymine-thymine (T-T) stabilization, a biotinylated stem-loop DNA probe, and a streptavidin-modified retroreflective Janus particle (SA-RJP). Two oligonucleotide probes, including a stem-loop DNA probe and an assistant DNA probe, were utilized. In the absence of Hg 2+ , the assistant DNA probe does not hybridize with the stem-loop probe due to their T-T mismatch, so the surface-immobilized stem-loop DNA probe remains a closed hairpin structure. In the presence of Hg 2+ , the DNA forms a double-stranded structure with the loop region via Hg 2+ -mediated T-T stabilization. This DNA hybridization induces stretching of the stem-loop DNA probe, exposing biotin. To translate these Hg 2+ -mediated structural changes in DNA probe into measurable signal, SA-RJP, an optical signaling label, is applied to recognize the exposed biotin. The number of biospecifically bound SA-RJPs is proportional to the concentration of Hg 2+ , so that the concentration of Hg 2+ can be quantitatively analyzed by counting the number of RJPs. Using the system, a highly selective and sensitive measurement of Hg 2+ was accomplished with a limit of detection of 0.027nM. Considering the simplified optical instrumentation required for retroreflection-based RJP counting, RJP-assisted Hg 2+ measurement can be accomplished in a much easier and inexpensive manner. Moreover, the detection of Hg 2+ in real drinking water samples including tap and commercial bottled water was successfully carried out. Copyright © 2018 Elsevier B.V. All rights reserved.

A fine-wire thermocouple probe for measurement of stagnation temperatures in real gas hypersonic flows of nitrogen

NASA Technical Reports Server (NTRS)

Hollis, Brian R.; Griffith, Wayland C.; Yanta, William J.

1991-01-01

A fine-wire thermocouple probe was used to determine freestream stagnation temperatures in hypersonic flows. Data were gathered in a N2 blowdown wind tunnel with runtimes of 1-5 s. Tests were made at supply pressures between 30 and 1400 atm and supply temperatures between 700 and 1900 K, with Mach numbers of 14 to 16. An iterative procedure requiring thermocouple data, pilot pressure measurements, and supply conditions was used to determine test cell stagnation temperatures. Probe conduction and radiation losses, as well as real gas behavior of N2, were accounted for during analysis. Temperature measurement error was found to be 5 to 10 percent. A correlation was drawn between thermocouple diameter Reynolds number and temperature recovery ratio. Transient probe behavior was studied and was found to be adequate in temperature gradients up to 1000 K/s.

Emergence of the Green’s Functions from Noise and Passive Acoustic Remote Sensing of Ocean Dynamics

DTIC Science & Technology

2009-09-30

Acoustic Remote Sensing of Ocean Dynamics Oleg A. Godin CIRES/Univ. of Colorado and NOAA/OAR/Earth System Research Lab., R/PSD99, 325 Broadway...characterization of a time-varying ocean where ambient acoustic noise is utilized as a probing signal. • To develop a passive remote sensing technique for...inapplicable. 3. To quantify degradation of performance of passive remote sensing techniques due to ocean surface motion and other variations of underwater

Influence of Wind Tunnel Noise on the Location of Boundary-Layer Transition on a Slender Cone at Mach Numbers from 0.2 to 5.5. Volume I. Experimental Methods and Summary of Results.

DTIC Science & Technology

1980-03-01

UNCLASSIFIED UNCLASSIFIED 20. ABSTRACT (Continued) either a traversing pitot pressure probe in contact with the cone surface or the flush-mounted...CONCLUDING REMARKS 46 REFERENCES 46 ILLUSTRATIONS Figure 1. 2. 3. 4. 5. 6. 7. 8. ’.. 9. i ~.. AEDC 10-deg Transition Cone Model 6 Pitot Pressure Probe ...Installation Sketch 9 Details of Pitot Pressure Probe Assembly 10 Typical Pitot Pressure Probe Sensing Tube/Transducer Frequency Response

Chemical sensing flow probe

DOEpatents

Laguna, George R.; Peter, Frank J.; Butler, Michael A.

1999-01-01

A new chemical probe determines the properties of an analyte using the light absorption of the products of a reagent/analyte reaction. The probe places a small reaction volume in contact with a large analyte volume. Analyte diffuses into the reaction volume. Reagent is selectively supplied to the reaction volume. The light absorption of the reaction in the reaction volume indicates properties of the original analyte. The probe is suitable for repeated use in remote or hostile environments. It does not require physical sampling of the analyte or result in significant regent contamination of the analyte reservoir.

Chemical sensing flow probe

DOEpatents

Laguna, G.R.; Peter, F.J.; Butler, M.A.

1999-02-16

A new chemical probe determines the properties of an analyte using the light absorption of the products of a reagent/analyte reaction. The probe places a small reaction volume in contact with a large analyte volume. Analyte diffuses into the reaction volume. Reagent is selectively supplied to the reaction volume. The light absorption of the reaction in the reaction volume indicates properties of the original analyte. The probe is suitable for repeated use in remote or hostile environments. It does not require physical sampling of the analyte or result in significant regent contamination of the analyte reservoir. 7 figs.

Novel Multisensor Probe for Monitoring Bladder Temperature During Locoregional Chemohyperthermia for Nonmuscle-Invasive Bladder Cancer: Technical Feasibility Study

PubMed Central

Geijsen, Debby E.; Zum Vörde Sive Vörding, Paul J.; Schooneveldt, Gerben; Sijbrands, Jan; Hulshof, Maarten C.; de la Rosette, Jean; de Reijke, Theo M.; Crezee, Hans

2013-01-01

Abstract Background and Purpose: The effectiveness of locoregional hyperthermia combined with intravesical instillation of mitomycin C to reduce the risk of recurrence and progression of intermediate- and high-risk nonmuscle-invasive bladder cancer is currently investigated in clinical trials. Clinically effective locoregional hyperthermia delivery necessitates adequate thermal dosimetry; thus, optimal thermometry methods are needed to monitor accurately the temperature distribution throughout the bladder wall. The aim of the study was to evaluate the technical feasibility of a novel intravesical device (multi-sensor probe) developed to monitor the local bladder wall temperatures during loco-regional C-HT. Materials and Methods: A multisensor thermocouple probe was designed for deployment in the human bladder, using special sensors to cover the bladder wall in different directions. The deployment of the thermocouples against the bladder wall was evaluated with visual, endoscopic, and CT imaging in bladder phantoms, porcine models, and human bladders obtained from obduction for bladder volumes and different deployment sizes of the probe. Finally, porcine bladders were embedded in a phantom and subjected to locoregional heating to compare probe temperatures with additional thermometry inside and outside the bladder wall. Results: The 7.5 cm thermocouple probe yielded optimal bladder wall contact, adapting to different bladder volumes. Temperature monitoring was shown to be accurate and representative for the actual bladder wall temperature. Conclusions: Use of this novel multisensor probe could yield a more accurate monitoring of the bladder wall temperature during locoregional chemohyperthermia. PMID:24112045

Examining the Effectiveness of Hacked, Commercial, Self-Tuning RFID Tags to Passively Sense the Volumetric Water Content of Soil

NASA Astrophysics Data System (ADS)

Stoddard, B. S.; Udell, C.; Selker, J. S.

2017-12-01

Currently available soil volumetric water content (VWC) sensors have several drawbacks that pose certain challenges for implementation on large scale for farms. Such issues include cost, scalability, maintenance, wires running through fields, and single-spot resolution. The development of a passive soil moisture sensing system utilizing Radio Frequency Identification (RFID) would allay many of these issues. The type of passive RFID tags discussed in this paper currently cost between 8 to 15 cents retail per tag when purchased in bulk. An incredibly cheap, scalable, low-maintenance, wireless, high-resolution system for sensing soil moisture would be possible if such tags were introduced into the agricultural world. This paper discusses both the use cases as well as examines one implementation of the tags. In 2015, RFID tag manufacturer SmarTrac started selling RFID moisture sensing tags for use in the automotive industry to detect leaks during quality assurance. We place those tags in soil at a depth of 4 inches and compared the moisture levels sensed by the RFID tags with the relative permittivity (ɛr) of the soil as measured by an industry-standard probe. Using an equation derived by Topp et al, we converted to VWC. We tested this over a wide range of moisture conditions and found a statistically significant, correlational relationship between the sensor values from the RFID tags and the probe's measurement of ɛr. We also identified a possible function for mapping vales from the RFID tag to the probe bounded by a reasonable margin of error.

Borehole geological assessment

NASA Technical Reports Server (NTRS)

Spuck, W. H., III (Inventor)

1979-01-01

A method and apparatus are discussed for performing geological assessments of a formation located along a borehole, and a boring tool that bores a pair of holes into the walls of the borehole and into the surrounding strata along with a pair of probes which are installed in the holes. One of the probes applies an input such as a current or pressured fluid, and the other probe senses a corresponding input which it receives from the strata.

Level monitoring system with pulsating sensor—Application to online level monitoring of dashpots in a fast breeder reactor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Malathi, N.; Sahoo, P., E-mail: sahoop@igcar.gov.in; Ananthanarayanan, R.

2015-02-15

An innovative continuous type liquid level monitoring system constructed by using a new class of sensor, viz., pulsating sensor, is presented. This device is of industrial grade and it is exclusively used for level monitoring of any non conducting liquid. This instrument of unique design is suitable for high resolution online monitoring of oil level in dashpots of a sodium-cooled fast breeder reactor. The sensing probe is of capacitance type robust probe consisting of a number of rectangular mirror polished stainless steel (SS-304) plates separated with uniform gaps. The performance of this novel instrument has been thoroughly investigated. The precision,more » sensitivity, response time, and the lowest detection limit in measurement using this device are <0.01 mm, ∼100 Hz/mm, ∼1 s, and ∼0.03 mm, respectively. The influence of temperature on liquid level is studied and the temperature compensation is provided in the instrument. The instrument qualified all recommended tests, such as environmental, electromagnetic interference and electromagnetic compatibility, and seismic tests prior to its deployment in nuclear reactor. With the evolution of this level measurement approach, it is possible to provide dashpot oil level sensors in fast breeder reactor for the first time for continuous measurement of oil level in dashpots of Control and Safety Rod Drive Mechanism during reactor operation.« less

Comparison of two fiber-optical temperature measurement systems in magnetic fields up to 9.4 Tesla.

PubMed

Buchenberg, Waltraud B; Dadakova, Tetiana; Groebner, Jens; Bock, Michael; Jung, Bernd

2015-05-01

Precise temperature measurements in the magnetic field are indispensable for MR safety studies and for temperature calibration during MR-guided thermotherapy. In this work, the interference of two commonly used fiber-optical temperature measurement systems with the static magnetic field B0 was determined. Two fiber-optical temperature measurement systems, a GaAs-semiconductor and a phosphorescent phosphor ceramic, were compared for temperature measurements in B0 . The probes and a glass thermometer for reference were placed in an MR-compatible tube phantom within a water bath. Temperature measurements were carried out at three different MR systems covering static magnetic fields up to B0  = 9.4T, and water temperatures were changed between 25°C and 65°C. The GaAs-probe significantly underestimated absolute temperatures by an amount related to the square of B0 . A maximum difference of ΔT = -4.6°C was seen at 9.4T. No systematic temperature difference was found with the phosphor ceramic probe. For both systems, the measurements were not dependent on the orientation of the sensor to B0 . Temperature measurements with the phosphor ceramic probe are immune to magnetic fields up to 9.4T, whereas the GaAs-probes either require a recalibration inside the MR system or a correction based on the square of B0. © 2014 Wiley Periodicals, Inc.

Combination probes for stagnation pressure and temperature measurements in gas turbine engines

NASA Astrophysics Data System (ADS)

Bonham, C.; Thorpe, S. J.; Erlund, M. N.; Stevenson, R. J.

2018-01-01

During gas turbine engine testing, steady-state gas-path stagnation pressures and temperatures are measured in order to calculate the efficiencies of the main components of turbomachinery. These measurements are acquired using fixed intrusive probes, which are installed at the inlet and outlet of each component at discrete point locations across the gas-path. The overall uncertainty in calculated component efficiency is sensitive to the accuracy of discrete point pressures and temperatures, as well as the spatial sampling across the gas-path. Both of these aspects of the measurement system must be considered if more accurate component efficiencies are to be determined. High accuracy has become increasingly important as engine manufacturers have begun to pursue small gains in component performance, which require efficiencies to be resolved to within less than  ± 1% . This article reports on three new probe designs that have been developed in a response to this demand. The probes adopt a compact combination arrangement that facilitates up to twice the spatial coverage compared to individual stagnation pressure and temperature probes. The probes also utilise novel temperature sensors and high recovery factor shield designs that facilitate improvements in point measurement accuracy compared to standard Kiel probes used in engine testing. These changes allow efficiencies to be resolved within  ± 1% over a wider range of conditions than is currently achievable with Kiel probes.

Tropical Storm Blas off the Pacific Coast of Mexico

NASA Image and Video Library

2004-07-14

Tropical Storm Blas as observed by the Atmospheric Infrared Sounder AIRS onboard NASA Aqua in the year 2004. The major contribution to radiation (infrared light) that AIRS channels sense comes from different levels in the atmosphere, depending upon the channel wavelength. To create the movie, a set of AIRS channels were selected which probe the atmosphere at progressively deeper levels. If there were no clouds, the color in each frame would be nearly uniform until the Earth's surface is encountered. The tropospheric air temperature warms at a rate of 6 K (about 11 F) for each kilometer of descent toward the surface. Thus the colors would gradually change from cold to warm as the movie progresses. Clouds block the infrared radiation. Thus wherever there are clouds we can penetrate no deeper in infrared. The color remains fixed as the movie progresses, for that area of the image is "stuck" to the cloud top temperature. The coldest temperatures around 220 K (about -65 F) come from altitudes of about 10 miles. http://photojournal.jpl.nasa.gov/catalog/PIA00436

40 CFR 60.386 - Test methods and procedures.

Code of Federal Regulations, 2010 CFR

2010-07-01

.... The sample volume for each run shall be at least 1.70 dscm (60 dscf). The sampling probe and filter... probe and filter temperature slightly above the effluent temperature (up to a maximum filter temperature...

40 CFR 60.386 - Test methods and procedures.

Code of Federal Regulations, 2014 CFR

2014-07-01

.... The sample volume for each run shall be at least 1.70 dscm (60 dscf). The sampling probe and filter... probe and filter temperature slightly above the effluent temperature (up to a maximum filter temperature...

40 CFR 60.386 - Test methods and procedures.

Code of Federal Regulations, 2012 CFR

2012-07-01

.... The sample volume for each run shall be at least 1.70 dscm (60 dscf). The sampling probe and filter... probe and filter temperature slightly above the effluent temperature (up to a maximum filter temperature...

40 CFR 60.386 - Test methods and procedures.

Code of Federal Regulations, 2011 CFR

2011-07-01

.... The sample volume for each run shall be at least 1.70 dscm (60 dscf). The sampling probe and filter... probe and filter temperature slightly above the effluent temperature (up to a maximum filter temperature...

Microwave dielectric behavior of vegetation material

NASA Technical Reports Server (NTRS)

Elrayes, Mohamed A.; Ulaby, Fawwaz T.

1987-01-01

The microwave dielectric behavior of vegetation was examined through the development of theoretical models involving dielectric dispersion by both bound and free water and supported by extensive dielectric measurements conducted over a wide range of conditions. The experimental data were acquired using an open-ended coaxial probe that was developed for sensing the dielectric constant of thin layers of materials, such as leaves, from measurements of the complex reflection coefficient using a network analyzer. The probe system was successfully used to record the spectral variation of the dielectric constant over a wide frequency range extending from 0.5 to 20.4 GHz at numerous temperatures between -40 to +40 C. The vegetation samples were measured over a wide range of moisture conditions. To model the dielectric spectrum of the bound water component of the water included in vegetation, dielectric measurements were made for several sucrose-water solutions as analogs for the situation in vegetation. The results were used in conjunction with the experimental data for leaves to determine some of the constant coefficients in the theoretical models. Two models, both of which provide good fit to the data, are proposed.

A Writing Template for Probing Students' Geological Sense of Place

ERIC Educational Resources Information Center

Clary, Renee M.; Wandersee, James H.

2006-01-01

Because many incoming geoscience students did not acknowledge their previous personal encounters with the earth's geological processes or products, we developed the Geological Sense of Place (GSP) template as a convenient way to assess students' earth science backgrounds through short answer, mini-essay, and induced associative responses. The GSP…

Dynamical sensitivity control of a single-spin quantum sensor.

PubMed

Lazariev, Andrii; Arroyo-Camejo, Silvia; Rahane, Ganesh; Kavatamane, Vinaya Kumar; Balasubramanian, Gopalakrishnan

2017-07-26

The Nitrogen-Vacancy (NV) defect in diamond is a unique quantum system that offers precision sensing of nanoscale physical quantities at room temperature beyond the current state-of-the-art. The benchmark parameters for nanoscale magnetometry applications are sensitivity, spectral resolution, and dynamic range. Under realistic conditions the NV sensors controlled by conventional sensing schemes suffer from limitations of these parameters. Here we experimentally show a new method called dynamical sensitivity control (DYSCO) that boost the benchmark parameters and thus extends the practical applicability of the NV spin for nanoscale sensing. In contrast to conventional dynamical decoupling schemes, where π pulse trains toggle the spin precession abruptly, the DYSCO method allows for a smooth, analog modulation of the quantum probe's sensitivity. Our method decouples frequency selectivity and spectral resolution unconstrained over the bandwidth (1.85 MHz-392 Hz in our experiments). Using DYSCO we demonstrate high-accuracy NV magnetometry without |2π| ambiguities, an enhancement of the dynamic range by a factor of 4 · 10 3 , and interrogation times exceeding 2 ms in off-the-shelf diamond. In a broader perspective the DYSCO method provides a handle on the inherent dynamics of quantum systems offering decisive advantages for NV centre based applications notably in quantum information and single molecule NMR/MRI.

Remote sensing of three-dimensional cirrus clouds from satellites: application to continuous-wave laser atmospheric transmission and backscattering.

PubMed

Liou, K N; Ou, Szu-Cheng; Takano, Yoshihide; Cetola, Jeffrey

2006-09-10

A satellite remote sensing methodology has been developed to retrieve 3D ice water content (IWC) and mean effective ice crystal size of cirrus clouds from satellite data on the basis of a combination of the conventional retrieval of cloud optical depth and particle size in a horizontal plane and a parameterization of the vertical cloud profile involving temperature from sounding and/or analysis. The inferred 3D cloud fields of IWC and mean effective ice crystal size associated with two impressive cirrus clouds that occurred in the vicinity of northern Oklahoma on 18 April 1997 and 9 March 2000, obtained from the Department of Energy's Atmospheric Radiation Measurement Program, have been validated against the ice crystal size distributions that were collected independently from collocated and coincident aircraft optical probe measurements. The 3D cloud results determined from satellite data have been applied to the simulation of cw laser energy propagation, and we show the significance of 3D cloud geometry and inhomogeneity and spherical atmosphere on the transmitted and backscattered laser powers. Finally, we demonstrate that the 3D cloud fields derived from satellite remote sensing can be used for the 3D laser transmission and backscattering model for tactical application.

Remote sensing of three-dimensional cirrus clouds from satellites: application to continuous-wave laser atmospheric transmission and backscattering

NASA Astrophysics Data System (ADS)

Liou, K. N.; Ou, Szu-Cheng; Takano, Yoshihide; Cetola, Jeffrey

2006-09-01

A satellite remote sensing methodology has been developed to retrieve 3D ice water content (IWC) and mean effective ice crystal size of cirrus clouds from satellite data on the basis of a combination of the conventional retrieval of cloud optical depth and particle size in a horizontal plane and a parameterization of the vertical cloud profile involving temperature from sounding and/or analysis. The inferred 3D cloud fields of IWC and mean effective ice crystal size associated with two impressive cirrus clouds that occurred in the vicinity of northern Oklahoma on 18 April 1997 and 9 March 2000, obtained from the Department of Energy's Atmospheric Radiation Measurement Program, have been validated against the ice crystal size distributions that were collected independently from collocated and coincident aircraft optical probe measurements. The 3D cloud results determined from satellite data have been applied to the simulation of cw laser energy propagation, and we show the significance of 3D cloud geometry and inhomogeneity and spherical atmosphere on the transmitted and backscattered laser powers. Finally, we demonstrate that the 3D cloud fields derived from satellite remote sensing can be used for the 3D laser transmission and backscattering model for tactical application.

Near-field fluorescence thermometry using highly efficient triple-tapered near-field optical fiber probe.

PubMed

Fujii, T; Taguchi, Y; Saiki, T; Nagasaka, Y

2012-12-01

A novel local temperature measurement method using fluorescence near-field optics thermal nanoscopy (Fluor-NOTN) has been developed. Fluor-NOTN enables nanoscale temperature measurement in situ by detecting the temperature-dependent fluorescence lifetime of CdSe quantum dots (QDs). In this paper, we report a novel triple-tapered near-field optical fiber probe that can increase the temperature measurement sensitivity of Fluor-NOTN. The performance of the proposed probe was numerically evaluated by the finite difference time domain method. Due to improvements in both the throughput and collection efficiency of near-field light, the sensitivity of the proposed probe was 1.9 times greater than that of typical double-tapered probe. The proposed shape of the triple-tapered core was successfully fabricated utilizing a geometrical model. The detected signal intensity of dried layers of QDs was greater by more than two orders than that of auto-fluorescence from the fiber core. In addition, the near-field fluorescence lifetime of the QDs and its temperature dependence were successfully measured by the fabricated triple-tapered near-field optical fiber probe. These measurement results verified the capability of the proposed triple-tapered near-field optical fiber probe to improve the collection efficiency of near-field fluorescence.

Enhancement of low-temperature thermometry by strong coupling

NASA Astrophysics Data System (ADS)

Correa, Luis A.; Perarnau-Llobet, Martí; Hovhannisyan, Karen V.; Hernández-Santana, Senaida; Mehboudi, Mohammad; Sanpera, Anna

2017-12-01

We consider the problem of estimating the temperature T of a very cold equilibrium sample. The temperature estimates are drawn from measurements performed on a quantum Brownian probe strongly coupled to it. We model this scenario by resorting to the canonical Caldeira-Leggett Hamiltonian and find analytically the exact stationary state of the probe for arbitrary coupling strength. In general, the probe does not reach thermal equilibrium with the sample, due to their nonperturbative interaction. We argue that this is advantageous for low-temperature thermometry, as we show in our model that (i) the thermometric precision at low T can be significantly enhanced by strengthening the probe-sampling coupling, (ii) the variance of a suitable quadrature of our Brownian thermometer can yield temperature estimates with nearly minimal statistical uncertainty, and (iii) the spectral density of the probe-sample coupling may be engineered to further improve thermometric performance. These observations may find applications in practical nanoscale thermometry at low temperatures—a regime which is particularly relevant to quantum technologies.

Spectral structure of laser light scattering revisited: bandwidths of nonresonant scattering lidars.

PubMed

She, C Y

2001-09-20

It is well known that scattering lidars, i.e., Mie, aerosol-wind, Rayleigh, high-spectral-resolution, molecular-wind, rotational Raman, and vibrational Raman lidars, are workhorses for probing atmospheric properties, including the backscatter ratio, aerosol extinction coefficient, temperature, pressure, density, and winds. The spectral structure of molecular scattering (strength and bandwidth) and its constituent spectra associated with Rayleigh and vibrational Raman scattering are reviewed. Revisiting the correct name by distinguishing Cabannes scattering from Rayleigh scattering, and sharpening the definition of each scattering component in the Rayleigh scattering spectrum, the review allows a systematic, logical, and useful comparison in strength and bandwidth between each scattering component and in receiver bandwidths (for both nighttime and daytime operation) between the various scattering lidars for atmospheric sensing.

OLED-based biosensing platform with ZnO nanoparticles for enzyme immobilization

NASA Astrophysics Data System (ADS)

Cai, Yuankun; Shinar, Ruth; Shinar, Joseph

2009-08-01

Organic light-emitting diode (OLED)-based sensing platforms are attractive for photoluminescence (PL)-based monitoring of a variety of analytes. Among the promising OLED attributes for sensing applications is the thin and flexible size and design of the OLED pixel array that is used for PL excitation. To generate a compact, fielddeployable sensor, other major sensor components, such as the sensing probe and the photodetector, in addition to the thin excitation source, should be compact. To this end, the OLED-based sensing platform was tested with composite thin biosensing films, where oxidase enzymes were immobilized on ZnO nanoparticles, rather than dissolved in solution, to generate a more compact device. The analytes tested, glucose, cholesterol, and lactate, were monitored by following their oxidation reactions in the presence of oxygen and their respective oxidase enzymes. During such reactions, oxygen is consumed and its residual concentration, which is determined by the initial concentration of the above-mentioned analytes, is monitored. The sensors utilized the oxygen-sensitive dye Pt octaethylporphyrin, embedded in polystyrene. The enzymes were sandwiched between two thin ZnO layers, an approach that was found to improve the stability of the sensing probes.

Convective heat flow probe

DOEpatents

Dunn, J.C.; Hardee, H.C.; Striker, R.P.

1984-01-09

A convective heat flow probe device is provided which measures heat flow and fluid flow magnitude in the formation surrounding a borehole. The probe comprises an elongate housing adapted to be lowered down into the borehole; a plurality of heaters extending along the probe for heating the formation surrounding the borehole; a plurality of temperature sensors arranged around the periphery of the probe for measuring the temperature of the surrounding formation after heating thereof by the heater elements. The temperature sensors and heater elements are mounted in a plurality of separate heater pads which are supported by the housing and which are adapted to be radially expanded into firm engagement with the walls of the borehole. The heat supplied by the heater elements and the temperatures measured by the temperature sensors are monitored and used in providing the desired measurements. The outer peripheral surfaces of the heater pads are configured as segments of a cylinder and form a full cylinder when taken together. A plurality of temperature sensors are located on each pad so as to extend along the length and across the width thereof, with a heating element being located in each pad beneath the temperature sensors. An expansion mechanism driven by a clamping motor provides expansion and retraction of the heater pads and expandable packet-type seals are provided along the probe above and below the heater pads.

Convective heat flow probe

DOEpatents

Dunn, James C.; Hardee, Harry C.; Striker, Richard P.

1985-01-01

A convective heat flow probe device is provided which measures heat flow and fluid flow magnitude in the formation surrounding a borehole. The probe comprises an elongate housing adapted to be lowered down into the borehole; a plurality of heaters extending along the probe for heating the formation surrounding the borehole; a plurality of temperature sensors arranged around the periphery of the probe for measuring the temperature of the surrounding formation after heating thereof by the heater elements. The temperature sensors and heater elements are mounted in a plurality of separate heater pads which are supported by the housing and which are adapted to be radially expanded into firm engagement with the walls of the borehole. The heat supplied by the heater elements and the temperatures measured by the temperature sensors are monitored and used in providing the desired measurements. The outer peripheral surfaces of the heater pads are configured as segments of a cylinder and form a full cylinder when taken together. A plurality of temperature sensors are located on each pad so as to extend along the length and across the width thereof, with a heating element being located in each pad beneath the temperature sensors. An expansion mechanism driven by a clamping motor provides expansion and retraction of the heater pads and expandable packer-type seals are provided along the probe above and below the heater pads.

Unsteady Flow Field in a Multistage Axial Flow Compressor

NASA Technical Reports Server (NTRS)

Suryavamshi, N.; Lakshminarayana, B.; Prato, J.

1997-01-01

The flow field in a multistage compressor is three-dimensional, unsteady, and turbulent with substantial viscous effects. Some of the specific phenomena that has eluded designers include the effects of rotor-stator and rotor-rotor interactions and the physics of mixing of velocity, pressure, temperature and velocity fields. An attempt was made, to resolve experimentally, the unsteady pressure and temperature fields downstream of the second stator of a multistage axial flow compressor which will provide information on rotor-stator interaction effects and the nature of the unsteadiness in an embedded stator of a three stage axial flow compressor. Detailed area traverse measurements using pneumatic five hole probe, thermocouple probe, semi-conductor total pressure probe (Kulite) and an aspirating probe downstream of the second stator were conducted at the peak efficiency operating condition. The unsteady data was then reduced through an ensemble averaging technique which splits the signal into deterministic and unresolved components. Auto and cross correlation techniques were used to correlate the deterministic total temperature and velocity components (acquired using a slanted hot-film probe at the same measurement locations) and the gradients, distributions and relative weights of each of the terms of the average passage equation were then determined. Based on these measurements it was observed that the stator wakes, hub leakage flow region, casing endwall suction surface corner region, and the casing endwall region away from the blade surfaces were the regions of highest losses in total pressure, lowest efficiency and highest levels of unresolved unsteadiness. The deterministic unsteadiness was found to be high in the hub and casing endwall regions as well as on the pressure side of the stator wake. The spectral distribution of hot-wire and kulite voltages shows that at least eight harmonics of all three rotor blade passing frequencies are present at this measurement location. In addition to the basic three rotor blade passing frequencies (R1, R2 and R3) and their harmonics, various difference frequencies such as (2R1 -R2) and (2R3-R2) and their harmonics are also observed. These difference frequencies are due to viscous and potential interactions between rotors 1, 2 and 3 which are sensed by both the total pressure and aspirating probes at this location. Significant changes occur to the stator exit flow features with passage of the rotor upstream of the stator. Because of higher convection speeds of the rotor wake on the suction surface of the downstream stator than on the pressure side, the chopped rotor wake was found to be arriving at different times on either side of the stator wake. As the rotor passes across the stator.

Probing Student Teachers' Subject Content Knowledge in Chemistry: Case Studies Using Dynamic Computer Models

ERIC Educational Resources Information Center

Toplis, Rob

2008-01-01

This paper reports case study research into the knowledge and understanding of chemistry for six secondary science student teachers. It combines innovative student-generated computer animations, using "ChemSense" software, with interviews to probe understanding of four common chemical processes used in the secondary school curriculum. Findings…

Detection of heavy metal ions in contaminated water by surface plasmon resonance based optical fibre sensor using conducting polymer and chitosan.

PubMed

Verma, Roli; Gupta, Banshi D

2015-01-01

Optical fibre surface plasmon resonance (SPR) based sensor for the detection of heavy metal ions in the drinking water is designed. Silver (Ag) metal and indium tin oxide (ITO) are used for the fabrication of the SPR probe which is further modified with the coating of pyrrole and chitosan composite. The sensor works on the wavelength interrogation technique and is capable of detecting trace amounts of Cd(2+), Pb(2+), and Hg(2+) heavy metal ions in contaminated water. Four types of sensing probes are fabricated and characterised for heavy metal ions out of these pyrrole/chitosan/ITO/Ag coated probe is found to be highly sensitive among all other probes. Further, the cadmium ions bind strongly to the sensing surface than other ions and due to this the sensor is highly sensitive for Cd(2+) ions. The sensor's performance is best for the low concentrations of heavy metal ions and its sensitivity decreases with the increasing concentration of heavy metal ions. Copyright © 2014 Elsevier Ltd. All rights reserved.

Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase.

PubMed

Tsutsui, Hidekazu; Jinno, Yuka; Tomita, Akiko; Niino, Yusuke; Yamada, Yoshiyuki; Mikoshiba, Katsuhiko; Miyawaki, Atsushi; Okamura, Yasushi

2013-09-15

  One of the most awaited techniques in modern physiology is the sensitive detection of spatiotemporal electrical activity in a complex network of excitable cells. The use of genetically encoded voltage probes has been expected to enable such analysis. However, in spite of recent progress, existing probes still suffer from low signal amplitude and/or kinetics too slow to detect fast electrical activity. Here, we have developed an improved voltage probe named Mermaid2, which is based on the voltage-sensor domain of the voltage-sensing phosphatase from Ciona intestinalis and Förster energy transfer between a pair of fluorescent proteins. In mammalian cells, Mermaid2 permits ratiometric readouts of fractional changes of more than 50% over a physiologically relevant voltage range with fast kinetics, and it was used to follow a train of action potentials at frequencies of up to 150 Hz. Mermaid2 was also able to detect single action potentials and subthreshold voltage responses in hippocampal neurons in vitro, in addition to cortical electrical activity evoked by sound stimuli in single trials in living mice.

A "signal on" protection-displacement-hybridization-based electrochemical hepatitis B virus gene sequence sensor with high sensitivity and peculiar adjustable specificity.

PubMed

Li, Fengqin; Xu, Yanmei; Yu, Xiang; Yu, Zhigang; He, Xunjun; Ji, Hongrui; Dong, Jinghao; Song, Yongbin; Yan, Hong; Zhang, Guiling

2016-08-15

One "signal on" electrochemical sensing strategy was constructed for the detection of a specific hepatitis B virus (HBV) gene sequence based on the protection-displacement-hybridization-based (PDHB) signaling mechanism. This sensing system is composed of three probes, one capturing probe (CP) and one assistant probe (AP) which are co-immobilized on the Au electrode surface, and one 3-methylene blue (MB) modified signaling probe (SP) free in the detection solution. One duplex are formed between AP and SP with the target, a specific HBV gene sequence, hybridizing with CP. This structure can drive the MB labels close to the electrode surface, thereby producing a large detection current. Two electrochemical testing techniques, alternating current voltammetry (ACV) and cyclic voltammetry (CV), were used for characterizing the sensor. Under the optimized conditions, the proposed sensor exhibits a high sensitivity with the detection limit of ∼5fM for the target. When used for the discrimination of point mutation, the sensor also features an outstanding ability and its peculiar high adjustability. Copyright © 2016 Elsevier B.V. All rights reserved.

Comparison of multiple enzyme activatable near infrared fluorescent molecular probes for detection and quantification of inflammation in murine colitis models

PubMed Central

Ding, Shengli; Blue, Randal E.; Morgan, Douglas R.; Lund, Pauline K.

2015-01-01

Background Activatable near-infrared fluorescent (NIRF) probes have been used for ex vivo and in vivo detection of intestinal tumors in animal models. We hypothesized that NIRF probes activatable by cathepsins or MMPs will detect and quantify dextran sulphate sodium (DSS) induced acute colonic inflammation in wild type (WT) mice or chronic colitis in IL-10 null mice ex vivo or in vivo. Methods WT mice given DSS, water controls and IL-10 null mice with chronic colitis were administered probes by retro-orbital injection. FMT2500 LX system imaged fresh and fixed intestine ex vivo and mice in vivo. Inflammation detected by probes was verified by histology and colitis scoring. NIRF signal intensity was quantified using 2D region of interest (ROI) ex vivo or 3D ROI-analysis in vivo. Results Ex vivo, seven probes tested yielded significant higher NIRF signals in colon of DSS treated mice versus controls. A subset of probes was tested in IL-10 null mice and yielded strong ex vivo signals. Ex vivo fluorescence signal with 680 series probes was preserved after formalin fixation. In DSS and IL-10 null models, ex vivo NIRF signal strongly and significantly correlated with colitis scores. In vivo, ProSense680, CatK680FAST and MMPsense680 yielded significantly higher NIRF signals in DSS treated mice than controls but background was high in controls. Conclusion Both cathepsin or MMP-activated NIRF-probes can detect and quantify colonic inflammation ex vivo. ProSense680 yielded the strongest signals in DSS colitis ex vivo and in vivo, but background remains a problem for in vivo quantification of colitis. PMID:24374874

A fusion-spliced near-field optical fiber probe using photonic crystal fiber for nanoscale thermometry based on fluorescence-lifetime measurement of quantum dots.

PubMed

Fujii, Takuro; Taguchi, Yoshihiro; Saiki, Toshiharu; Nagasaka, Yuji

2011-01-01

We have developed a novel nanoscale temperature-measurement method using fluorescence in the near-field called fluorescence near-field optics thermal nanoscopy (Fluor-NOTN). Fluor-NOTN enables the temperature distributions of nanoscale materials to be measured in vivo/in situ. The proposed method measures temperature by detecting the temperature dependent fluorescence lifetimes of Cd/Se quantum dots (QDs). For a high-sensitivity temperature measurement, the auto-fluorescence generated from a fiber probe should be reduced. In order to decrease the noise, we have fabricated a novel near-field optical-fiber probe by fusion-splicing a photonic crystal fiber (PCF) and a conventional single-mode fiber (SMF). The validity of the novel fiber probe was assessed experimentally by evaluating the auto-fluorescence spectra of the PCF. Due to the decrease of auto-fluorescence, a six- to ten-fold increase of S/N in the near-field fluorescence lifetime detection was achieved with the newly fabricated fusion-spliced near-field optical fiber probe. Additionally, the near-field fluorescence lifetime of the quantum dots was successfully measured by the fabricated fusion-spliced near-field optical fiber probe at room temperature, and was estimated to be 10.0 ns.

Advanced Human Factors Engineering Tool Technologies.

DTIC Science & Technology

1988-03-01

charger/ AC adapter immersible probe air temperature probe surface temperature probe . * Sling psychrometer , MSA or Taylor 1328A * Aspirating... psychrometer , Model PP-100 or CP-147, Psychro-Dyne * Wet-bulb-heat-stress monitor, Model B&K 1219, Briel & Kjaer Transducer, Model B&K MM 0030 (3 each), Brijel

A near-infrared fluorescent probe for rapid detection of carbon monoxide in living cells.

PubMed

Yan, Liqiang; Nan, Ding; Lin, Cheng; Wan, Yi; Pan, Qiang; Qi, Zhengjian

2018-09-05

A near-infrared (NIR) and colorimetric fluorescent probe system was developed for Carbon Monoxide (CO) via a Pd 0 -mediated Tsuji-Trost reaction. In this probe, phenoxide anion formation (DPCO - ) was acted as the signal unit and an allyl carbonate group was used as the recognition unit. This non-fluorescent probe molecule can release the relevant fluorophore after conversion of Pd 2+ to Pd 0 by CO. The probe system including probe 1 and Pd 2+ can be used for "naked-eye" detection of CO, and exhibited high selectivity to CO over various other sensing objects. More importantly, the probe system has great potential for fluorescence imaging of intracellular CO in living cells. Copyright © 2018 Elsevier B.V. All rights reserved.

Protein adsorption/desorption and antibody binding stoichiometry on silicon interferometric biosensors examined with TOF-SIMS

NASA Astrophysics Data System (ADS)

Gajos, Katarzyna; Budkowski, Andrzej; Petrou, Panagiota; Pagkali, Varvara; Awsiuk, Kamil; Rysz, Jakub; Bernasik, Andrzej; Misiakos, Konstantinos; Raptis, Ioannis; Kakabakos, Sotirios

2018-06-01

Time-of-flight secondary ion mass spectrometry has been employed to examine, with biomolecular discrimination, sensing arm areas (20 μm × 600 μm) of integrated onto silicon chips Mach-Zehnder interferometers aiming to optimize their biofunctionalization with regard to indirect immunochemical (competitive) detection of ochratoxin A. Sensing areas are examined after: modification with (3-aminopropyl)triethoxysilane, spotting of OTA-ovalbumin conjugate (probe) from solutions with different concentration, blocking with bovine serum albumin, reaction with OTA-specific mouse monoclonal antibody followed by goat anti-mouse IgG secondary antibody. Component mass loadings of all proteins involved in immunodetection are determined from TOF-SIMS micro-analysis combined with ellipsometry of planar surfaces. These data show that partial desorption of surface-bound probe and blocking protein takes place upon primary immunoreaction to a degree that depends on probe concentration in spotting solution. Taking into account this desorption, apparent binding stoichiometry of both antibodies in immune complexes formed onto chip surface is determined more accurately than the respective evaluation based on real-time sensor response. In addition, mass loadings for probe and secondary antibody is observed to saturate for optimum probe concentrations. Also, principal component analysis of TOF-SIMS data could resolve both immunoreactions and biofunctionalization and discriminate surfaces prepared with optimum probe concentrations from those prepared using suboptimum ones.

Demonstration of Temperature Dependent Energy Migration in Dual-Mode YVO4: Ho3+/Yb3+ Nanocrystals for Low Temperature Thermometry

PubMed Central

Kumar Mahata, Manoj; Koppe, Tristan; Kumar, Kaushal; Hofsäss, Hans; Vetter, Ulrich

2016-01-01

A dual mode rare-earth based vanadate material (YVO4: Ho3+/Yb3+), prepared through ethylene glycol assisted hydrothermal method, demonstrating both downconversion and upconversion, along with systematic investigation of the luminescence spectroscopy within 12–300 K is presented herein. The energy transfer processes have been explored via steady-state and time-resolved spectroscopic measurements and explained in terms of rate equation description and temporal evolution below room temperature. The maximum time for energy migration from host to rare earth (Ho3+) increases (0.157 μs to 0.514 μs) with the material’s temperature decreasing from 300 K to 12 K. The mechanism responsible for variation of the transients’ character is discussed through thermalization and non-radiative transitions in the system. More significantly, the temperature of the nanocrystals was determined using not only the thermally equilibrated radiative intra-4f transitions of Ho3+ but also the decay time and rise time of vanadate and Ho3+ energy levels. Our studies show that the material is highly suitable for temperature sensing below room temperature. The maximum relative sensor sensitivity using the rise time of Ho3+ energy level (5F4/5S2) is 1.35% K−1, which is the highest among the known sensitivities for luminescence based thermal probes. PMID:27805060

Analyte-Triggered DNA-Probe Release from a Triplex Molecular Beacon for Nanopore Sensing.

PubMed

Guo, Bingyuan; Sheng, Yingying; Zhou, Ke; Liu, Quansheng; Liu, Lei; Wu, Hai-Chen

2018-03-26

A new nanopore sensing strategy based on triplex molecular beacon was developed for the detection of specific DNA or multivalent proteins. The sensor is composed of a triplex-forming molecular beacon and a stem-forming DNA component that is modified with a host-guest complex. Upon target DNA hybridizing with the molecular beacon loop or multivalent proteins binding to the recognition elements on the stem, the DNA probe is released and produces highly characteristic current signals when translocated through α-hemolysin. The frequency of current signatures can be used to quantify the concentrations of the target molecules. This sensing approach provides a simple, quick, and modular tool for the detection of specific macromolecules with high sensitivity and excellent selectivity. It may find useful applications in point-of-care diagnostics with a portable nanopore kit in the future. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Theoretical study of chromophores for biological sensing: Understanding the mechanism of rhodol based multi-chromophoric systems

NASA Astrophysics Data System (ADS)

Rivera-Jacquez, Hector J.; Masunov, Artëm E.

2018-06-01

Development of two-photon fluorescent probes can aid in visualizing the cellular environment. Multi-chromophore systems display complex manifolds of electronic transitions, enabling their use for optical sensing applications. Time-Dependent Density Functional Theory (TDDFT) methods allow for accurate predictions of the optical properties. These properties are related to the electronic transitions in the molecules, which include two-photon absorption cross-sections. Here we use TDDFT to understand the mechanism of aza-crown based fluorescent probes for metals sensing applications. Our findings suggest changes in local excitation in the rhodol chromophore between unbound form and when bound to the metal analyte. These changes are caused by a charge transfer from the aza-crown group and pyrazol units toward the rhodol unit. Understanding this mechanism leads to an optimized design with higher two-photon excited fluorescence to be used in medical applications.

Theoretical study of chromophores for biological sensing: Understanding the mechanism of rhodol based multi-chromophoric systems.

PubMed

Rivera-Jacquez, Hector J; Masunov, Artëm E

2018-06-05

Development of two-photon fluorescent probes can aid in visualizing the cellular environment. Multi-chromophore systems display complex manifolds of electronic transitions, enabling their use for optical sensing applications. Time-Dependent Density Functional Theory (TDDFT) methods allow for accurate predictions of the optical properties. These properties are related to the electronic transitions in the molecules, which include two-photon absorption cross-sections. Here we use TDDFT to understand the mechanism of aza-crown based fluorescent probes for metals sensing applications. Our findings suggest changes in local excitation in the rhodol chromophore between unbound form and when bound to the metal analyte. These changes are caused by a charge transfer from the aza-crown group and pyrazol units toward the rhodol unit. Understanding this mechanism leads to an optimized design with higher two-photon excited fluorescence to be used in medical applications. Copyright © 2018 Elsevier B.V. All rights reserved.

Improved understanding of the ball-pen probe through particle-in-cell simulations

NASA Astrophysics Data System (ADS)

Murphy-Sugrue, S.; Harrison, J.; Walkden, N. R.; Bryant, P.; Bradley, J. W.

2017-05-01

Ball-pen probes (BPP) have been deployed in the SOL of numerous tokamak experiments and low-temperature magnetised plasmas to make direct measurements of the plasma potential and electron temperature. Despite strong empirical evidence for the success of the BPP it lacks a theoretical underpinning of its collection mechanism. In this paper we investigate the capability of the probe to measure the plasma potential by means of particle-in-cell simulations. The BPP is found to float at a potential offset from the plasma potential by a factor {T}{{e}}{α }{{BPP}}. By simulating BPPs and Langmuir probes, excellent agreement has been found between the measured electron temperature and the specified source temperature. The transport mechanism for both ions and electrons has been determined. E × B drifts are observed to drive electrons and ions down the tunnel. This mechanism is sensitive to the diameter of the probe.

High Temperatures Health Monitoring of the Condensed Water Height in Steam Pipe Systems

NASA Technical Reports Server (NTRS)

Lih, Shyh-Shiuh; Bar-Cohen, Yoseph; Lee, Hyeong Jae; Badescu, Mircea; Bao, Xiaoqi; Sherrit, Stewart; Takano, Nobuyuki; Ostlund, Patrick; Blosiu, Julian

2013-01-01

Ultrasonic probes were designed, fabricated and tested for high temperature health monitoring system. The goal of this work was to develop the health monitoring system that can determine the height level of the condensed water through the pipe wall at high temperature up to 250 deg while accounting for the effects of surface perturbation. Among different ultrasonic probe designs, 2.25 MHz probes with air backed configuration provide satisfactory results in terms of sensitivity, receiving reflections from the target through the pipe wall. A series of tests were performed using the air-backed probes under irregular conditions, such as surface perturbation and surface disturbance at elevated temperature, to qualify the developed ultrasonic system. The results demonstrate that the fabricated air-backed probes combined with advanced signal processing techniques offer the capability of health monitoring of steam pipe under various operating conditions.

High temperature integrated ultrasonic shear and longitudinal wave probes

NASA Astrophysics Data System (ADS)

Ono, Y.; Jen, C.-K.; Kobayashi, M.

2007-02-01

Integrated ultrasonic shear wave probes have been designed and developed using a mode conversion theory for nondestructive testing and characterization at elevated temperatures. The probes consisted of metallic substrates and high temperature piezoelectric thick (>40μm) films through a paint-on method. Shear waves are generated due to mode conversion from longitudinal to shear waves because of reflection inside the substrate having a specific shape. A novel design scheme is proposed to reduce the machining time of substrates and thick film fabrication difficulty. A probe simultaneously generating and receiving both longitudinal and shear waves is also developed and demonstrated. In addition, a shear wave probe using a clad buffer rod consisting of an aluminum core and stainless steel cladding has been developed. All the probes were tested and successfully operated at 150°C.

Body Temperature Measurements for Metabolic Phenotyping in Mice.

PubMed

Meyer, Carola W; Ootsuka, Youichirou; Romanovsky, Andrej A

2017-01-01

Key Points Rectal probing is subject to procedural bias. This method is suitable for first-line phenotyping, provided probe depth and measurement duration are standardized. It is also useful for detecting individuals with out-of-range body temperatures (during hypothermia, torpor).The colonic temperature attained by inserting the probe >2 cm deep is a measure of deep (core) body temperature.IR imaging of the skin is useful for detecting heat leaks and autonomous thermoregulatory alterations, but it does not measure body temperature.Temperature of the hairy or shaved skin covering the inter-scapular brown adipose tissue can be used as a measure of BAT thermogenesis. However, obtaining such measurements of sufficient quality is very difficult, and interpreting them can be tricky. Temperature differences between the inter-scapular and lumbar areas can be a better measure of the thermogenic activity of inter-scapular brown adipose tissue.Implanted probes for precise determination of BAT temperature (changes) should be fixed close to the Sulzer's vein. For measurement of BAT thermogenesis, core body temperature and BAT temperature should be recorded simultaneously.Tail temperature is suitable to compare the presence or absence of vasoconstriction or vasodilation.Continuous, longitudinal monitoring of core body temperature is preferred over single probing, as the readings are taken in a non-invasive, physiological context.Combining core body temperature measurements with metabolic rate measurements yields insights into the interplay between heat production and heat loss (thermal conductance), potentially revealing novel thermoregulatory phenotypes. Endothermic organisms rely on tightly balanced energy budgets to maintain a regulated body temperature and body mass. Metabolic phenotyping of mice, therefore, often includes the recording of body temperature. Thermometry in mice is conducted at various sites, using various devices and measurement practices, ranging from single-time probing to continuous temperature imaging. Whilst there is broad agreement that body temperature data is of value, procedural considerations of body temperature measurements in the context of metabolic phenotyping are missing. Here, we provide an overview of the various methods currently available for gathering body temperature data from mice. We explore the scope and limitations of thermometry in mice, with the hope of assisting researchers in the selection of appropriate approaches, and conditions, for comprehensive mouse phenotypic analyses.

Application of active distribute temperature sensing and fiber optic as sensors to determinate the unsaturated hydraulic conductivity curve

NASA Astrophysics Data System (ADS)

Zubelzu, Sergio; Rodriguez-Sinobas, Leonor; Sobrino, Fernando

2017-04-01

The development of methodologies for the characterization of soil water content through the use of distribute temperature sensing and fiber optic cable has allowed for modelling with high temporal and spatial accuracy water movement in soils. One of the advantage of using fiber optic as a sensor, compared with the traditional point water probes, is the possibility to measure the variable continuously along the cable every 0.125 m (up to a cable length of 1500) and every second. Traditionally, applications based on fiber optic as a soil water sensor apply the active heated fiber optic technique AHFO to follow the evolution soil water content during and after irrigation events or for hydrologic characterization. However, this paper accomplishes an original experience by using AHFO as a sensor to characterize the soil hydraulic conductivity curve in subsaturated conditions. The non lineal nature between the hidraulic conductivity curve and soil water, showing high slope in the range close to saturation ) favors the AHFO a most suitable sensor due to its ability to measure the variable at small time and length intervals. Thus, it is possible to obtain accurate and a large number of data to be used to estimate the hydraulic conductivity curve from de water flow general equation by numerical methods. Results are promising and showed the feasibility of this technique to estimate the hydraulic conductivity curve for subsaturated soils .

Sn 1-x VxOy thin films deposited by pulsed laser ablation for gas sensing devices

NASA Astrophysics Data System (ADS)

Duhalde, Stella; Vignolo, M. F.; Quintana, G.; Mercader, R.; Lamagna, Antonino

2000-02-01

Polycrystalline pure and V-doped SnO2 thin films have been prepare by pulsed laser deposition (PLD) on Si substrates, with a Si3Ni4 buffered layer. PLD technique, under proper conditions, has probed to produce nanocrystalline-structured materials, which are suitable for gas sensing. In this work we analyze the role of V doping in the structural properties and in the electrical conductivity of the films. The deposition temperature was fixed at 600 degrees C and the films were grown in oxygen atmosphere. The films resulted nanocrystalline with 50 to 120 nm average grain size connected by necks with high surface areas. The microstructural and electronic properties of all the films were analyzed using scanning-electron microscopy, x-ray diffraction and conversion electron Moessbauer spectroscopy. Electrical conductance in a dynamic regime in dry synthetic air has been evaluated as a function of temperature. Moessbauer spectra reveal the presence of 15 percent of Sn2+ in the 5at. percent V-doped films. At about 340 degrees C, a strong increase in the conductivity of the films occurs. Possible explanations are that thermal energy could excite electrons from the vanadium ions into the crystal's conduction band or promotes the diffusion of surface oxygen vacancies towards the bulk, increasing strongly the conductivity of the film.

Au nanoparticle monolayers covered with sol-gel oxide thin films: optical and morphological study.

PubMed

Della Gaspera, Enrico; Karg, Matthias; Baldauf, Julia; Jasieniak, Jacek; Maggioni, Gianluigi; Martucci, Alessandro

2011-11-15

In this work, we provide a detailed study of the influence of thermal annealing on submonolayer Au nanoparticle deposited on functionalized surfaces as standalone films and those that are coated with sol-gel NiO and TiO(2) thin films. The systems are characterized through the use of UV-vis absorption, X-ray diffraction (XRD), atomic force microscopy (AFM), scanning electron microscopy (SEM), and spectroscopic ellipsometry. The surface plasmon resonance peak of the Au nanoparticles was found to red-shift and increase in intensity with increasing surface coverage, an observation that is directly correlated to the complex refractive index properties of Au nanoparticle layers. The standalone Au nanoparticles sinter at 200 °C, and a relationship between the optical properties and the annealing temperature is presented. When overcoated with sol-gel metal oxide films (NiO, TiO(2)), the optical properties of the Au nanoparticles are strongly affected by the metal oxide, resulting in an intense red shift and broadening of the plasmon band; moreover, the temperature-driven sintering is strongly limited by the metal oxide layer. Optical sensing tests for ethanol vapor are presented as one possible application, showing reversible sensing dynamics and confirming the effect of Au nanoparticles in increasing the sensitivity and in providing a wavelength dependent response, thus confirming the potential use of such materials as optical probes.

Hg2+-reactive double hydrophilic block copolymer assemblies as novel multifunctional fluorescent probes with improved performance.

PubMed

Hu, Jinming; Li, Changhua; Liu, Shiyong

2010-01-19

We report on novel type of responsive double hydrophilic block copolymer (DHBC)-based multifunctional chemosensors to Hg(2+) ions, pH, and temperatures and investigate the effects of thermo-induced micellization on the detection sensitivity. Well-defined DHBCs bearing rhodamine B-based Hg(2+)-reactive moieties (RhBHA) in the thermo-responsive block, poly(ethylene oxide)-b-poly(N-isopropylacrylamide-co-RhBHA) (PEO-b-P(NIPAM-co-RhBHA)), were synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. Nonfluorescent RhBHA moieties are subjected to selective ring-opening reaction upon addition of Hg(2+) ions or lowering solution pH, producing highly fluorescent acyclic species. Thus, at room temperature PEO-b-P(NIPAM-co-RhBHA) DHBCs can serve as water-soluble multifunctional and efficient fluorescent chemosensors to Hg(2+) ions and pH. Upon heating above the lower critical solution temperature (approximately 36 degrees C) of the PNIPAM block, they self-assemble into micelles possessing P(NIPAM-co-RhBHA) cores and well-solvated PEO coronas, which were fully characterized by dynamic and static laser light scattering. It was found that the detection sensitivity to Hg(2+) ions and pH could be dramatically improved at elevated temperatures due to fluorescence enhancement of RhBHA residues in the acyclic form, which were embedded within hydrophobic cores of thermo-induced micellar aggregates. This work represents a proof-of-concept example of responsive DHBC-based multifunctional fluorescent chemosensors for the highly efficient detection of Hg(2+) ions, pH, and temperatures with tunable detection sensitivity. Compared to reaction-based small molecule Hg(2+) probes in previous literature reports, the integration of stimuli-responsive block copolymers with well-developed small molecule-based selective sensing moieties in the current study are expected to exhibit preferred advantages including enhanced detection sensitivity, water dispersibility, biocompatibility, facile incorporation into devices, and the ability of further functionalization for targeted imaging and detection.

Effect of the magnetic field on measurements of the electron density and temperature by cylindrical probes in the Earth's ionosphere

NASA Astrophysics Data System (ADS)

Gubsky, V. F.

2009-12-01

In the 1960s and 1970s, quite simply produced cylindrical Langmuir probes were used in the USSR both on satellites (Kosmos-378, Intercosmos-2, -4, -8, -10, -19) and to measure the electron density and temperature on vertical launched rockets (Vertical’-4, -6, -10) within the Intercosmos program. These measurements were first made at middle latitudes. With increasing inclination of the orbits of launched satellites (satellites had no stabilization), falling sections were sometimes observed on probe characteristics in the electron saturation region. The Intercosmos-Bulgaria-1300 satellite, which was stabilized along three axes and was equipped with a cylindrical probe whose longitudinal axis was always directed downward to the Earth, was launched in 1981. This satellite allowed definite conclusions on the effect of the geomagnetic field on the form of the probe characteristic and, hence, on the determination of the electron density and temperature. Probe characteristics with falling sections are presented. These measurements are compared with those performed in a laboratory plasma. The appearance of negative sections on the probe characteristics is shown to be due to the effect of the geomagnetic field. The degree of this effect depends both on the electron density and temperature and on the probe voltage.

Development of a 3-wire probe for the simultaneous measurement of turbulent velocity, concentration and temperature fields

NASA Astrophysics Data System (ADS)

Hewes, Alaïs; Mydlarski, Laurent

2015-11-01

The present work focuses on the design and optimization of a probe used to simultaneously measure the velocity, concentration and temperature fields in a turbulent jet. The underlying principles of this sensor are based in thermal-anemometry techniques, and the design of this 3-wire probe builds off the previous work of Sirivat and Warhaft, J. Fluid Mech., 1982. In the first part of this study, the effect of different overheat ratios in the first two wires (called the ``interference'' or ``Way-Libby'' probe - used to infer velocity and concentration) are investigated. Of particular interest is their effect on the quality of the resulting calibration, as well as the measured velocity and concentration data. Four different overheat ratio pairs for the two wires comprising the interference probe are studied. In the second part of this work, a third wire, capable of detecting temperature fluctuations, is added to the 3-wire probe. The optimal configuration of this probe, including wire type and overheat ratio for the third wire, is studied and the simultaneously-measured velocity, concentration, and temperature data (e.g. spectra, PDFs) for different probe configurations are presented. Supported by the Natural Sciences and Engineering Research Council of Canada (Grant 217184).

Development of high temperature acoustic emission sensing system using fiber Bragg grating

NASA Astrophysics Data System (ADS)

Pang, Dandan; Sui, Qingmei; Wang, Ming; Guo, Dongmei; Sai, Yaozhang

2018-03-01

In some applications in structural health monitoring (SHM), the acoustic emission (AE) detection technology is used in the high temperature environment. In this paper, a high-temperature-resistant AE sensing system is developed based on the fiber Bragg grating (FBG) sensor. A novel high temperature FBG AE sensor is designed with a high signal-to-noise ratio (SNR) compared with the traditional FBG AE sensor. The output responses of the designed sensors with different sensing fiber lengths also are investigated both theoretically and experimentally. Excellent AE detection results are obtained using the proposed FBG AE sensing system over a temperature range from 25 ° to 200 °. The experimental results indicate that this FBG AE sensing system can well meet the application requirement in AE detecting areas at high temperature.

Fluorescent porous silicon biological probes with high quantum efficiency and stability.

PubMed

Tu, Chang-Ching; Chou, Ying-Nien; Hung, Hsiang-Chieh; Wu, Jingda; Jiang, Shaoyi; Lin, Lih Y

2014-12-01

We demonstrate porous silicon biological probes as a stable and non-toxic alternative to organic dyes or cadmium-containing quantum dots for imaging and sensing applications. The fluorescent silicon quantum dots which are embedded on the porous silicon surface are passivated with carboxyl-terminated ligands through stable Si-C covalent bonds. The porous silicon bio-probes have shown photoluminescence quantum yield around 50% under near-UV excitation, with high photochemical and thermal stability. The bio-probes can be efficiently conjugated with antibodies, which is confirmed by a standard enzyme-linked immunosorbent assay (ELISA) method.

Hybrid SnO2/TiO2 Nanocomposites for Selective Detection of Ultra-Low Hydrogen Sulfide Concentrations in Complex Backgrounds

PubMed Central

Larin, Alexander; Womble, Phillip C.; Dobrokhotov, Vladimir

2016-01-01

In this paper, we present a chemiresistive metal oxide (MOX) sensor for detection of hydrogen sulfide. Compared to the previous reports, the overall sensor performance was improved in multiple characteristics, including: sensitivity, selectivity, stability, activation time, response time, recovery time, and activation temperature. The superior sensor performance was attributed to the utilization of hybrid SnO2/TiO2 oxides as interactive catalytic layers deposited using a magnetron radio frequency (RF) sputtering technique. The unique advantage of the RF sputtering for sensor fabrication is the ability to create ultra-thin films with precise control of geometry, morphology and chemical composition of the product of synthesis. Chemiresistive films down to several nanometers can be fabricated as sensing elements. The RF sputtering technique was found to be very robust for bilayer and multilayer oxide structure fabrication. The geometry, morphology, chemical composition and electronic structure of interactive layers were evaluated in relation to their gas sensing performance, using scanning electron microscopy (SEM), X-ray diffraction technique (XRD), atomic force microscopy (AFM), Energy Dispersive X-ray Spectroscopy (EDAX), UV visible spectroscopy, and Kelvin probe measurements. A sensor based on multilayer SnO2/TiO2 catalytic layer with 10% vol. content of TiO2 demonstrated the best gas sensing performance in all characteristics. Based on the pattern relating material’s characteristics to gas sensing performance, the optimization strategy for hydrogen sulfide sensor fabrication was suggested. PMID:27618900

Improved Estimation of Electron Temperature from Rocket-borne Impedance Probes

NASA Astrophysics Data System (ADS)

Rowland, D. E.; Wolfinger, K.; Stamm, J. D.

2017-12-01

The impedance probe technique is a well known method for determining high accuracy measurements of electron number density in the Earth's ionosphere. We present analysis of impedance probe data from several sounding rockets at low, mid-, and auroral latitudes, including high cadence estimates of the electron temperature, derived from analytical fits to the antenna impedance curves. These estimates compare favorably with independent estimates from Langmuir Probes, but at much higher temporal and spatial resolution, providing a capability to resolve small-scale temperature fluctuations. We also present some considerations for the design of impedance probes, including assessment of the effects of resonance damping due to rocket motion, effects of wake and spin modulation, and aspect angle to the magnetic field.

A modeling-based assessment of acousto-optic sensing for monitoring high-intensity focused ultrasound lesion formation

NASA Astrophysics Data System (ADS)

Adams, Matthew Tyler

Real-time acousto-optic (AO) sensing---a dual-wave modality that combines ultrasound with diffuse light to probe the optical properties of turbid media---has been demonstrated to non-invasively detect changes in ex vivo tissue optical properties during high-intensity focused ultrasound (HIFU) exposure. The AO signal indicates the onset of lesion formation and predicts resulting lesion volumes. Although proof-of-concept experiments have been successful, many of the underlying parameters and mechanisms affecting thermally induced optical property changes and the AO detectability of HIFU lesion formation are not well understood. In thesis, a numerical simulation was developed to model the AO sensing process and capture the relevant acoustic, thermal, and optical transport processes. The simulation required data that described how optical properties changed with heating. Experiments were carried out where excised chicken breast was exposed to thermal bath heating and changes in the optical absorption and scattering spectra (500 nm--1100 nm) were measured using a scanning spectrophotometer and an integrating sphere assembly. Results showed that the standard thermal dose model currently used for guiding HIFU treatments needs to be adjusted to describe thermally induced optical property changes. To model the entire AO process, coupled models were used for ultrasound propagation, tissue heating, and diffusive light transport. The angular spectrum method was used to model the acoustic field from the HIFU source. Spatial-temporal temperature elevations induced by the absorption of ultrasound were modeled using a finite-difference time-domain solution to the Pennes bioheat equation. The thermal dose model was then used to determine optical properties based on the temperature history. The diffuse optical field in the tissue was then calculated using a GPU-accelerated Monte Carlo algorithm, which accounted for light-sound interactions and AO signal detection. The simulation was used to determine the optimal design for an AO guided HIFU system by evaluating the robustness of the systems signal to changes in tissue thickness, lesion optical contrast, and lesion location. It was determined that AO sensing is a clinically viable technique for guiding the ablation of large volumes and that real-time sensing may be feasible in the breast and prostate.

Disaggregation Of Passive Microwave Soil Moisture For Use In Watershed Hydrology Applications

NASA Astrophysics Data System (ADS)

Fang, Bin

In recent years the passive microwave remote sensing has been providing soil moisture products using instruments on board satellite/airborne platforms. Spatial resolution has been restricted by the diameter of antenna which is inversely proportional to resolution. As a result, typical products have a spatial resolution of tens of kilometers, which is not compatible for some hydrological research applications. For this reason, the dissertation explores three disaggregation algorithms that estimate L-band passive microwave soil moisture at the subpixel level by using high spatial resolution remote sensing products from other optical and radar instruments were proposed and implemented in this investigation. The first technique utilized a thermal inertia theory to establish a relationship between daily temperature change and average soil moisture modulated by the vegetation condition was developed by using NLDAS, AVHRR, SPOT and MODIS data were applied to disaggregate the 25 km AMSR-E soil moisture to 1 km in Oklahoma. The second algorithm was built on semi empirical physical models (NP89 and LP92) derived from numerical experiments between soil evaporation efficiency and soil moisture over the surface skin sensing depth (a few millimeters) by using simulated soil temperature derived from MODIS and NLDAS as well as AMSR-E soil moisture at 25 km to disaggregate the coarse resolution soil moisture to 1 km in Oklahoma. The third algorithm modeled the relationship between the change in co-polarized radar backscatter and the remotely sensed microwave change in soil moisture retrievals and assumed that change in soil moisture was a function of only the canopy opacity. The change detection algorithm was implemented using aircraft based the remote sensing data from PALS and UAVSAR that were collected in SMPAVEX12 in southern Manitoba, Canada. The PALS L-band h-polarization radiometer soil moisture retrievals were disaggregated by combining them with the PALS and UAVSAR L-band hh-polarization radar spatial resolutions of 1500 m and 5 m/800 m, respectively. All three algorithms were validated using ground measurements from network in situ stations or handheld hydra probes. The validation results demonstrate the practicability on coarse resolution passive microwave soil moisture products.

d-PET-controlled “off-on” Polarity-sensitive Probes for Reporting Local Hydrophilicity within Lysosomes

NASA Astrophysics Data System (ADS)

Zhu, Hao; Fan, Jiangli; Mu, Huiying; Zhu, Tao; Zhang, Zhen; Du, Jianjun; Peng, Xiaojun

2016-10-01

Polarity-sensitive fluorescent probes are powerful chemical tools for studying biomolecular structures and activities both in vitro and in vivo. However, the lack of “off-on” polarity-sensing probes has limited the accurate monitoring of biological processes that involve an increase in local hydrophilicity. Here, we design and synthesize a series of “off-on” polarity-sensitive fluorescent probes BP series consisting of the difluoroboron dippyomethene (BODIPY) fluorophore connected to a quaternary ammonium moiety via different carbon linkers. All these probes showed low fluorescence quantum yields in nonpolar solution but became highly fluorescent in polar media. BP-2, which contains a two-carbon linker and a trimethyl quaternary ammonium, displayed a fluorescence intensity and quantum yield that were both linearly correlated with solvent polarity. In addition, BP-2 exhibited high sensitivity and selectivity for polarity over other environmental factors and a variety of biologically relevant species. BP-2 can be synthesized readily via an unusual Mannich reaction followed by methylation. Using electrochemistry combined with theoretical calculations, we demonstrated that the “off-on” sensing behavior of BP-2 is primarily due to the polarity-dependent donor-excited photoinduced electron transfer (d-PET) effect. Live-cell imaging established that BP-2 enables the detection of local hydrophilicity within lysosomes under conditions of lysosomal dysfunction.

Glucose oxidase probe as a surface-enhanced Raman scattering sensor for glucose.

PubMed

Qi, Guohua; Wang, Yi; Zhang, Biying; Sun, Dan; Fu, Cuicui; Xu, Weiqing; Xu, Shuping

2016-10-01

Glucose oxidase (GOx) possessing a Raman-active chromophore (flavin adenine dinucleotide) is used as a signal reporter for constructing a highly specific "turn off" surface-enhanced Raman scattering (SERS) sensor for glucose. This sensing chip is made by the electrostatic assembly of GOx over silver nanoparticle (Ag NP)-functionalized SERS substrate through a positively charged polyelectrolyte linker under the pH of 6.86. To trace glucose in blood serum, owing to the reduced pH value caused by the production of gluconic acid in the GOx-catalyzed oxidation reaction, the bonding force between GOx and polyelectrolyte weakens, making GOx drop off from the sensing chip. As a result, the SERS intensity of GOx on the chip decreases along with the concentration of glucose. This glucose SERS sensor exhibits excellent selectivity based on the specific GOx/glucose catalysis reaction and high sensitivity to 1.0 μM. The linear sensing range is 2.0-14.0 mM, which also meets the requirement on the working range of the human blood glucose detection. Using GOx as a probe shows superiority over other organic probes because GOx almost has no toxicity to the biological system. This sensing mechanism can be applied for intracellular in vivo SERS monitoring of glucose in the future. Graphical abstract Glucose oxidase is used as a Raman signal reporter for constructing a highly specific glucose surface-enhanced Raman scattering (SERS) sensor.

Novel biocompatible hydrogel nanoparticles: generation and size-tuning of nanoparticles by the formation of micelle templates obtained from thermo-responsive monomers mixtures

NASA Astrophysics Data System (ADS)

Khandadash, Raz; Machtey, Victoria; Shainer, Inbal; Gottlieb, Hugo E.; Gothilf, Yoav; Ebenstein, Yuval; Weiss, Aryeh; Byk, Gerardo

2014-12-01

Biocompatible hydrogel nanoparticles are prepared by polymerization and cross-linking of N-isopropyl acrylamide in a micelle template formed by block copolymers macro-monomers at high temperature. Different monomer ratios form, at high temperature, well-defined micelles of different sizes which are further polymerized leading to nanoparticles with varied sizes from 20 to 390 nm. Physico-chemical characterization of the nanoparticles demonstrates their composition and homogeneity. The NPs were tested in vitro and in vivo biocompatibility assays, and their lack of toxicity was proven. The NPs can be labeled with fluorescent probes, and their intracellular fate can be visualized and quantified using confocal microscopy. Their uptake by live stem cells and distribution in whole developing animals is reported. On the basis of our results, a mechanism of nanoparticle formation is suggested. The lack of toxicity makes these nanoparticles especially attractive for biological applications such as screening and bio-sensing.

2C.07: INVOLVEMENT OF THE RENIN-ANGIOTENSIN SYSTEM IN A PREMATURE AGING MOUSE MODEL.

PubMed

Van Thiel, B S; Ridwan, Y; Garrelds, I M; Vermeij, M; Groningen, M C Clahsen-Van; Danser, A H J; Essers, J; Van Der Pluijm, I

2015-06-01

Changes in the renin-angiotensin system (RAS), known for its critical role in the regulation of blood pressure and sodium homeostasis, may contribute to aging and age-related diseases. Here we characterized the RAS and kidney pathology in mice with genomic instability due to a defective nucleotide excision repair gene (Ercc1d/- mice). These mice display premature features of aging, including vascular dysfunction. Studies were performed in male and female Ercc1d/- mice and their wild type controls (Ercc1+/+) at the age of 12 or 18 weeks before and after treatment with losartan. The renin-activatable near-infrared fluorescent probe ReninSense 680™ was applied in vivo to allow non-invasive imaging of renin activity. Plasma renin concentrations (PRC) were additionally measured ex vivo by quantifying Ang I generation in the presence of excess angiotensinogen. Kidneys were harvested and examined for markers of aging, and albumin was determined in urine. Kidneys of 12-week old Ercc1d/- mice showed signs of aging, including tubular anisokaryosis, cell-senescence and increased apoptosis. This was even more pronounced at the age of 18 weeks. Yet, urinary albumin was normal at 12 weeks. The ReninSense 680™ probe showed increased intrarenal renin activity in Ercc1d/- mice versus Ercc1+/+ mice, both at 12 and 18 weeks of age, while PRC in these mice tended to be lower compared to Ercc1+/+ mice. Renin was higher in male than female mice, both in the kidney and in plasma, and losartan increased kidney and plasma renin in both Ercc1d/- and Ercc1+/+ mice. Rapidly aging Ercc1d/- mice display an activated intrarenal RAS, as evidenced by the increased fluorescence detected with the ReninSense 680™ probe. This increased RAS activity may contribute to the disturbed kidney pathology in these mice. The increased intrarenal activity detected with the ReninSense 680™ probe in male vs. female mice, as well as after losartan treatment, are in full agreement with the literature, and thus not only validate the specificity of the probe, but also support its use for longitudinal imaging of altered RAS signaling in aging.

Modeling Regolith Temperatures and Volatile Ice Processes (Invited)

NASA Astrophysics Data System (ADS)

Mellon, M. T.

2013-12-01

Surface and subsurface temperatures are an important tool for exploring the distribution and dynamics of volatile ices on and within planetary regoliths. I will review thermal-analysis approaches and recent applications in the studies of volatile ice processes. Numerical models of regolith temperatures allow us to examine the response of ices to periodic and secular changes in heat sources such as insolation. Used in conjunction with spatially and temporally distributed remotely-sensed temperatures, numerical models can: 1) constrain the stability and dynamics of volatile ices; 2) define the partitioning between phases of ice, gas, liquid, and adsorbate; and 3) in some instances be used to probe the distribution of ice hidden from view beneath the surface. The vapor pressure of volatile ices (such as water, carbon dioxide, and methane) depends exponentially on temperature. Small changes in temperature can result in transitions between stable phases. Cyclic temperatures and the propagation of thermal waves into the subsurface can produce a strong hysteresis in the population and partitioning of various phases (such as between ice, vapor, and adsorbate) and result in bulk transport. Condensation of ice will also have a pronounced effect on the thermal properties of otherwise loose particulate regolith. Cementing grains at their contacts through ice deposition will increase the thermal conductivity, and may enhance the stability of additional ice. Likewise sintering of grains within a predominantly icy regolith will increase the thermal conductivity. Subsurface layers that result from ice redistribution can be discriminated by remote sensing when combined with numerical modeling. Applications of these techniques include modeling of seasonal carbon dioxide frosts on Mars, predicting and interpreting the subsurface ice distribution on Mars and in Antarctica, and estimating the current depth of ice-rich permafrost on Mars. Additionally, understanding cold trapping ices in regions of the regolith of airless bodies, such as Mercury and the Moon, are aided by numerical modeling of regolith temperatures. Thermally driven sublimation of volatiles (water ice on Mars and more exotic species on icy moons in the outer solar system) can result in terrain degradation and collapse.

Active radiometer for self-calibrated furnace temperature measurements

DOEpatents

Woskov, Paul P.; Cohn, Daniel R.; Titus, Charles H.; Wittle, J. Kenneth; Surma, Jeffrey E.

1996-01-01

Radiometer with a probe beam superimposed on its field-of-view for furnace temperature measurements. The radiometer includes a heterodyne millimeter/submillimeter-wave receiver including a millimeter/submillimeter-wave source for probing. The receiver is adapted to receive radiation from a surface whose temperature is to be measured. The radiation includes a surface emission portion and a surface reflection portion which includes the probe beam energy reflected from the surface. The surface emission portion is related to the surface temperature and the surface reflection portion is related to the emissivity of the surface. The simultaneous measurement of surface emissivity serves as a real time calibration of the temperature measurement.

Probing Gravitational Sensitivity in Biological Systems Using Magnetic Body Forces

NASA Technical Reports Server (NTRS)

Guevorkian, Karine; Wurzel, Sam; Mihalusova, Mariana; Valles, Jim

2003-01-01

At Brown University, we are developing the use of magnetic body forces as a means to simulate variable gravity body forces on biological systems. This tool promises new means to probe gravi-sensing and the gravi-response of biological systems. It also has the potential as a technique for screening future systems for space flight experiments.

Juno observes the dynamics of Jupiter's atmosphere

NASA Astrophysics Data System (ADS)

Ingersoll, Andrew P.; Juno Science Team

2017-10-01

Jupiter is a photogenic planet, but our knowledge of the deep atmosphere is limited. Remote sensing observations have traditionally probed within and above the cloud tops, which are in the 0.5-1.0 bar pressure range. Dynamical models have focused on explaining this data set. Microwave observations from Earth probe down to the 5-10 bar range, which overlaps with the predicted base of the water cloud. The Galileo probe yielded data on winds, composition, temperature gradients, clouds, radiant flux, and lightning down to 22 bars, but only at one place on the planet. Further, the traditional observations are constrained to cover low and middle latitudes. In contrast, Juno's camera and infrared radiometer, JunoCam and JIRAM, have yielded images of the poles that show cyclonic vortices in polygonal arrangements. Juno's microwave radiometer yields latitude-altitude cross sections that show dynamical features of the ammonia distribution down to 50-100 bars. And Jupiter's gravity field yields information about the winds at thousands of km depth, where the pressures are tens of kbars. In this talk I will summarize the Juno observations that pertain to the dynamics of Jupiter's atmosphere and I will offer some of my own interpretations. The new data raise as many questions as answers, but that is as it should be. As Ed Stone said during a Voyager encounter, "If we knew all the answers before we got there, we wouldn't be learning anything."

Highly Efficient Multiple-Anchored Fluorescent Probe for the Detection of Aniline Vapor Based on Synergistic Effect: Chemical Reaction and PET.

PubMed

Jiao, Zinuo; Zhang, Yu; Xu, Wei; Zhang, Xiangtao; Jiang, Haibo; Wu, Pengcheng; Fu, Yanyan; He, Qingguo; Cao, Huimin; Cheng, Jiangong

2017-05-26

A multiple-anchored fluorescent probe ((((hexane-1,6-diylbis(2,7-bis(4-formyl)-phenyl)-9H-fluorine-9,9-diyl))-bis(hexane-6,1-diyl))-bis(9H-carbazole-9,3,6-triyl))-tetrakis(benzene-4,1-diyl))-tetraformyl-(8FP-2F) with eight aldehyde groups was designed and synthesized. The molecule has four branches and highly twisted structure. Furthermore, it tends to self-assemble into nanospheres, which is beneficial for gaseous analyte penetration and high fluorescence quantum efficiency. Among gaseous analytes, detection of aniline vapor is extraordinarily important in the control of environmental issues and human diseases. Herein, 8FP-2F was introduced to detect aniline vapor with distinguished sensitivity and selectivity via simple Schiff base reaction at room temperature. After exposure to saturate aniline vapor, the 89% fluorescence of 8FP-2F was quenched in 50 s and the detection limit was as low as 3 ppb. Further study showed the suitable HOMO/LUMO energy levels and matched orbital symmetry between probe and aniline molecules ensured chemical reaction and PET process work together. The synergistic effect resulted in a significant sensing performance and fluorescence quenching toward aniline vapor. Moreover, the multiple active sites structure of 8FP-2F means it could be applied for constructing many interesting structures and highly efficient organic optoelectronic functional materials.

In situ temperature measurements with thermocouple probes during laser interstitial thermotherapy (LITT): quantification and correction of a measurement artifact.

PubMed

Manns, F; Milne, P J; Gonzalez-Cirre, X; Denham, D B; Parel, J M; Robinson, D S

1998-01-01

The purpose of this work was to quantify the magnitude of an artifact induced by stainless steel thermocouple probes in temperature measurements made in situ during experimental laser interstitial thermo-therapy (LITT). A procedure for correction of this observational error is outlined. A CW Nd:YAG laser system emitting 20W for 25-30 s delivered through a fiber-optic probe was used to create localized heating. The temperature field around the fiber-optic probe during laser irradiation was measured every 0.3 s in air, water, 0.4% intralipid solution, and fatty cadaver pig tissue, with a field of up to fifteen needle thermocouple probes. Direct absorption of Nd:YAG laser radiation by the thermocouple probes induced an overestimation of the temperature, ranging from 1.8 degrees C to 118.6 degrees C in air, 2.2 degrees C to 9.9 degrees C in water, 0.7 C to 4.7 C in intralipid and 0.3 C to 17.9 C in porcine tissue after irradiation at 20W for 30 s and depending on the thermocouple location. The artifact in porcine tissue was removed by applying exponential and linear fits to the measured temperature curves. Light absorption by thermocouple probes can induce a significant artifact in the measurement of laser-induced temperature increases. When the time constant of the thermocouple effect is much smaller than the thermal relaxation time of the surrounding tissue, the artifact can be accurately quantified. During LITT experiments where temperature differences of a few degrees are significant, the thermocouple artifact must be removed in order to be able accurately to predict the treatment outcome.

NARSTO EPA SS BALTIMORE JHU MET DATA

Atmospheric Science Data Center

2018-04-09

... Meteorological Station Instrument:  Temperature Probe Humidity Probe Cup Anemometer Rain Gauge Sonic ...   E arthdata Search Parameters:  Air Temperature Humidity Surface Winds Precipitation Amount Heat Flux ...

Hybrid femtosecond/picosecond rotational coherent anti-Stokes Raman scattering temperature and concentration measurements using two different picosecond-duration probes.

PubMed

Kearney, Sean P; Scoglietti, Daniel J; Kliewer, Christopher J

2013-05-20

A hybrid fs/ps pure-rotational CARS scheme is characterized in furnace-heated air at temperatures from 290 to 800 K. Impulsive femtosecond excitation is used to prepare a rotational Raman coherence that is probed with a ps-duration beam generated from an initially broadband fs pulse that is bandwidth limited using air-spaced Fabry-Perot etalons. CARS spectra are generated using 1.5- and 7.0-ps duration probe beams with corresponding coarse and narrow spectral widths. The spectra are fitted using a simple phenomenological model for both shot-averaged and single-shot measurements of temperature and oxygen mole fraction. Our single-shot temperature measurements exhibit high levels of precision and accuracy when the spectrally coarse 1.5-ps probe beam is used, demonstrating that high spectral resolution is not required for thermometry. An initial assessment of concentration measurements in air is also provided, with best results obtained using the higher resolution 7.0-ps probe. This systematic assessment of the hybrid CARS technique demonstrates its utility for practical application in low-temperature gas-phase systems.

Method for determining thermal conductivity and thermal capacity per unit volume of earth in situ

DOEpatents

Poppendiek, Heinz F.

1982-01-01

A method for determining the thermal conductivity of the earth in situ is based upon a cylindrical probe (10) having a thermopile (16) for measuring the temperature gradient between sets of thermocouple junctions (18 and 20) of the probe after it has been positioned in a borehole and has reached thermal equilibrium with its surroundings, and having means (14) for heating one set of thermocouple junctions (20) of the probe at a constant rate while the temperature gradient of the probe is recorded as a rise in temperature over several hours (more than about 3 hours). A fluid annulus thermally couples the probe to the surrounding earth. The recorded temperature curves are related to the earth's thermal conductivity, k.sub..infin., and to the thermal capacity per unit volume, (.gamma.c.sub.p).sub..infin., by comparison with calculated curves using estimates of k.sub..infin. and (.gamma.c.sub.p).sub..infin. in an equation which relates these parameters to a rise in the earth's temperature for a known and constant heating rate.

Closed-field capacitive liquid level sensor

DOEpatents

Kronberg, James W.

1998-01-01

A liquid level sensor based on a closed field circuit comprises a ring oscillator using a symmetrical array of plate units that creates a displacement current. The displacement current varies as a function of the proximity of a liquid to the plate units. The ring oscillator circuit produces an output signal with a frequency inversely proportional to the presence of a liquid. A continuous liquid level sensing device and a two point sensing device are both proposed sensing arrangements. A second set of plates may be located inside of the probe housing relative to the sensing plate units. The second set of plates prevent any interference between the sensing plate units.

Closed-field capacitive liquid level sensor

DOEpatents

Kronberg, J.W.

1998-03-03

A liquid level sensor based on a closed field circuit comprises a ring oscillator using a symmetrical array of plate units that creates a displacement current. The displacement current varies as a function of the proximity of a liquid to the plate units. The ring oscillator circuit produces an output signal with a frequency inversely proportional to the presence of a liquid. A continuous liquid level sensing device and a two point sensing device are both proposed sensing arrangements. A second set of plates may be located inside of the probe housing relative to the sensing plate units. The second set of plates prevent any interference between the sensing plate units. 12 figs.

Closed-field capacitive liquid level sensor

DOEpatents

Kronberg, J.W.

1995-01-01

A liquid level sensor based on a closed field circuit comprises a ring oscillator using a symmetrical array of plate units that creates a displacement current. The displacement current varies as a function of the proximity of a liquid to the plate units. The ring oscillator circuit produces an output signal with a frequency inversely proportional to the presence of a liquid. A continuous liquid level sensing device and a two point sensing device are both proposed sensing arrangements. A second set of plates may be located inside of the probe housing relative to the sensing plate units. The second set of plates prevent any interference between the sensing plate units.

Development of Simple Designs of Multitip Probe Diagnostic Systems for RF Plasma Characterization

PubMed Central

Naz, M. Y.; Shukrullah, S.; Ghaffar, A.; Rehman, N. U.

2014-01-01

Multitip probes are very useful diagnostics for analyzing and controlling the physical phenomena occurring in low temperature discharge plasmas. However, DC biased probes often fail to perform well in processing plasmas. The objective of the work was to deduce simple designs of DC biased multitip probes for parametric study of radio frequency plasmas. For this purpose, symmetric double probe, asymmetric double probe, and symmetric triple probe diagnostic systems and their driving circuits were designed and tested in an inductively coupled plasma (ICP) generated by a 13.56 MHz radio frequency (RF) source. Using I-V characteristics of these probes, electron temperature, electron number density, and ion saturation current was measured as a function of input power and filling gas pressure. An increasing trend was noticed in electron temperature and electron number density for increasing input RF power whilst a decreasing trend was evident in these parameters when measured against filling gas pressure. In addition, the electron energy probability function (EEPF) was also studied by using an asymmetric double probe. These studies confirmed the non-Maxwellian nature of the EEPF and the presence of two groups of the energetic electrons at low filling gas pressures. PMID:24683326

Detonation wave detection probe including parallel electrodes on a flexible backing strip

DOEpatents

Uher, Kenneth J.

1995-01-01

A device for sensing the occurrence of destructive events and events involving mechanical shock in a non-intrusive manner. A pair of electrodes is disposed in a parallel configuration on a backing strip of flexible film. Electrical circuitry is used to sense the time at which an event causes electrical continuity between the electrodes or, with a sensor configuration where the electrodes are shorted together, to sense the time at which electrical continuity is lost.

Amino-Functionalized Luminescent Metal-Organic Framework Test Paper for Rapid and Selective Sensing of SO2 Gas and Its Derivatives by Luminescence Turn-On Effect.

PubMed

Wang, Meng; Guo, Lin; Cao, Dapeng

2018-03-06

Rapid and selective sensing of sulfur dioxide (SO 2 ) gas has attracted more and more attention because SO 2 not only causes environmental pollution but also severely affects the health of human beings. Here we report an amino-functionalized luminescent metal-organic framework (MOF) material (i.e., MOF-5-NH 2 ) and further investigate its sensing property for SO 2 gas and its derivatives as a luminescent probe. The results indicate that the MOF-5-NH 2 probe can selectively and sensitively sense SO 2 derivatives (i.e., SO 3 2- ) in real time by a luminescence turn-on effect with a lower detection limit of 0.168 ppm and a response time of less than 15 s. Importantly, the luminescence turn-on phenomenon can be observed by the naked eye. We also assembled MOF-5-NH 2 into a test paper to achieve the aim of portable detection, and the lower-limit concentration of the test paper for sensing SO 2 in real time was found to be about 0.05 ppm. Moreover, MOF-5-NH 2 also shows good anti-interference ability, strong luminescence stability, and reusability, which means that this material is an excellent sensing candidate. The amino functionalization may also provide a modification strategy to design luminescent sensors for other atmospheric pollutants.

Emissive H-Aggregates of an Ultrafast Molecular Rotor: A Promising Platform for Sensing Heparin.

PubMed

Mudliar, Niyati H; Singh, Prabhat K

2016-11-23

Constructing "turn on" fluorescent probes for heparin, a most widely used anticoagulant in clinics, from commercially available materials is of great importance, but remains challenging. Here, we report the formation of a rarely observed emissive H-aggregate of an ultrafast molecular rotor dye, Thioflavin-T, in the presence of heparin, which provides an excellent platform for simple, economic and rapid fluorescence turn-on sensing of heparin. Generally, H-aggregates are considered as serious problem in the field of biomolecular sensing, owing to their poorly emissive nature resulting from excitonic interaction. To the best of our knowledge, this is the first report, where contrastingly, the turn-on emission from the H-aggregates has been utilized in the biomolecule sensing scheme, and enables a very efficient and selective detection of a vital biomolecule and a drug with its extensive medical applications, i.e., heparin. Our sensor system offers several advantages including, emission in the biologically advantageous red-region, dual sensing, i.e., both by fluorimetry and colorimetry, and most importantly constructed from in-expensive commercially available dye molecule, which is expected to impart a large impact on the sensing field of heparin. Our system displays good performance in complex biological media of serum samples. The novel Thioflavin-T aggregate emission could be also used to probe the interaction of heparin with its only clinically approved antidote, Protamine.

On the use of the double floating probe method to infer the difference between the electron and the heavy particles temperatures in an atmospheric pressure, vortex-stabilized nitrogen plasma jet

NASA Astrophysics Data System (ADS)

Prevosto, L.; Kelly, H.; Mancinelli, B. R.

2014-05-01

Sweeping double probe measurements in an atmospheric pressure direct current vortex-stabilized plasma jet are reported (plasma conditions: 100 A discharge current, N2 gas flow rate of 25 Nl/min, thoriated tungsten rod-type cathode, copper anode with 5 mm inner diameter). The interpretation of the double probe characteristic was based on a generalization of the standard double floating probe formulae for non-uniform plasmas coupled to a non-equilibrium plasma composition model. Perturbations caused by the current to the probe together with collisional and thermal processes inside the probe perturbed region were taken into account. Radial values of the average electron and heavy particle temperatures as well as the electron density were obtained. The calculation of the temperature values did not require any specific assumption about a temperature relationship between different particle species. An electron temperature of 10 900 ± 900 K, a heavy particle temperature of 9300 ± 900 K, and an electron density of about 3.5 × 1022 m-3 were found at the jet centre at 3.5 mm downstream from the torch exit. Large deviations from kinetic equilibrium were found toward the outer border of the plasma jet. These results showed good agreement with those previously reported by the authors by using a single probe technique. The calculations have shown that this method is particularly useful for studying spraying-type plasma torches operated at power levels of about 15 kW.

On the use of the double floating probe method to infer the difference between the electron and the heavy particles temperatures in an atmospheric pressure, vortex-stabilized nitrogen plasma jet.

PubMed

Prevosto, L; Kelly, H; Mancinelli, B R

2014-05-01

Sweeping double probe measurements in an atmospheric pressure direct current vortex-stabilized plasma jet are reported (plasma conditions: 100 A discharge current, N2 gas flow rate of 25 Nl/min, thoriated tungsten rod-type cathode, copper anode with 5 mm inner diameter). The interpretation of the double probe characteristic was based on a generalization of the standard double floating probe formulae for non-uniform plasmas coupled to a non-equilibrium plasma composition model. Perturbations caused by the current to the probe together with collisional and thermal processes inside the probe perturbed region were taken into account. Radial values of the average electron and heavy particle temperatures as well as the electron density were obtained. The calculation of the temperature values did not require any specific assumption about a temperature relationship between different particle species. An electron temperature of 10,900 ± 900 K, a heavy particle temperature of 9300 ± 900 K, and an electron density of about 3.5 × 10(22) m(-3) were found at the jet centre at 3.5 mm downstream from the torch exit. Large deviations from kinetic equilibrium were found toward the outer border of the plasma jet. These results showed good agreement with those previously reported by the authors by using a single probe technique. The calculations have shown that this method is particularly useful for studying spraying-type plasma torches operated at power levels of about 15 kW.

Radio Remote Sensing of Coronal Mass Ejections: Implications for Parker Solar Probe and Solar Orbiter

NASA Astrophysics Data System (ADS)

Kooi, J. E.; Thomas, N. C.; Guy, M. B., III; Spangler, S. R.

2017-12-01

Coronal mass ejections (CMEs) are fast-moving magnetic field structures of enhanced plasma density that play an important role in space weather. The Solar Orbiter and Parker Solar Probe will usher in a new era of in situ measurements, probing CMEs within distances of 60 and 10 solar radii, respectively. At the present, only remote-sensing techniques such as Faraday rotation can probe the plasma structure of CMEs at these distances. Faraday rotation is the change in polarization position angle of linearly polarized radiation as it propagates through a magnetized plasma (e.g. a CME) and is proportional to the path integral of the electron density and line-of-sight magnetic field. In conjunction with white-light coronagraph measurements, Faraday rotation observations have been used in recent years to determine the magnetic field strength of CMEs. We report recent results from simultaneous white-light and radio observations made of a CME in July 2015. We made radio observations using the Karl G. Jansky Very Large Array (VLA) at 1 - 2 GHz frequencies of a set of radio sources through the solar corona at heliocentric distances that ranged between 8 - 23 solar radii. These Faraday rotation observations provide a priori estimates for comparison with future in situ measurements made by the Solar Orbiter and Parker Solar Probe. Similar Faraday rotation observations made simultaneously with observations by the Solar Orbiter and Parker Solar Probe in the future could provide information about the global structure of CMEs sampled by these probes and, therefore, aid in understanding the in situ measurements.

Experimenting with Temperature Probes.

ERIC Educational Resources Information Center

Roth, Wolff-Michael

1989-01-01

Presented are four activities which are designed to familiarize children with the multiple uses of computers and help them learn about heat and temperature using temperature probes. Included are the tempering effect of water, heat capacity, caloric content of foods, and weather. Hardware and software are discussed. (CW)

Measurement of atmospheric pressure microplasma jet with Langmuir probes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Xu, Kunning G., E-mail: gabe.xu@uah.edu; Doyle, Steven J.

2016-09-15

A radio frequency argon microplasma jet at atmospheric-pressure is characterized using Langmuir probes. While optical methods are the typical diagnostic for these small scale plasmas, the simplicity and low cost of Langmuir probes makes them an attractive option. The plasma density and electron temperature are measured using existing high-pressure Langmuir probe theories developed for flames and arcs. The density and temperature vary from 1 × 10{sup 16} to 1 × 10{sup 19} m{sup −3} and 2.3 to 4.4 eV, respectively, depending on the operating condition. The density decreases while the electron temperature increases with axial distance from the jet exit. Themore » applicability of the probe theories as well as the effect of collisionality and jet mixing is discussed.« less

Temperature sensing in shock-heated evaporating aerosol using wavelength-modulation absorption spectroscopy of CO2 near 2.7 µm

NASA Astrophysics Data System (ADS)

Ren, Wei; Jeffries, Jay B.; Hanson, Ronald K.

2010-10-01

A tunable diode laser sensor with a detection bandwidth of 40 kHz is developed for measuring the time-varying gas temperature of CO2 during the evaporation of shock-heated hydrocarbon fuel aerosol. Normalized wavelength-modulation spectroscopy with second-harmonic detection (WMS-2f/1f) is used to probe R(28) and P(70) transitions in the ν1 + ν3 combination band of CO2 near 2.7 µm. The fixed-center-wavelength WMS sensor was first validated in a shock tube with non-reactive CO2/Ar gas mixtures, yielding an accuracy of better than 1.5% over the entire range of 650-1500 K. The sensor was then evaluated in a well-controlled aerosol flow cell, demonstrating the potential for precise gas temperature measurement even when aerosol scattering attenuates more than 99% of the incident light. Applications of this sensor for accurate temperature measurement of evaporating n-dodecane aerosol were then performed in an aerosol shock tube. The time-resolved temperature variation due to the evaporation of fuel droplets was accurately captured without using an off-resonant laser to account for the extinction from droplet scattering. Measured temperatures confirmed the accuracy of the gasdynamic model used to calculate the pre- and post-evaporation shock conditions, as needed in shock tube studies on combustion chemistry.

Probe Science: When It Has to Be In-situ

NASA Technical Reports Server (NTRS)

Colaprete, Anthony

2013-01-01

Sometimes remote sensing just isn't enough. Some critical science questions can only (or at least best) be answered with in-situ observations. Also, in-situ measurements are often necessary to calibrate or verify remote observations. It is in these instances that planetary probes are necessary. There is little doubt that the measurements these probes provide are critical. However, in an age when the duration of most planetary missions is measured in years and the number of terabytes of data returned is seen as a measure of value and success, the relatively short life and low data volumes of a probe missions is sometimes seen as a discriminating disadvantage. This talk will review the scientific value of probe missions and how future probe missions are critical to addressing fundamental questions about our solar system.

Effects of cold temperatures on the excitability of rat trigeminal ganglion neurons that are not for cold-sensing

PubMed Central

Kanda, Hirosato; Gu, Jianguo G.

2016-01-01

Except a small population of primary afferent neurons for sensing cold to generate the sensations of innocuous and noxious cold, it is generally believed that cold temperatures suppress the excitability of other primary afferent neurons that are not for cold-sensing. These not-for-cold-sensing neurons include the majority of non-nociceptive and nociceptive afferent neurons. In the present study we have found that not-for-cold-sensing neurons of rat trigeminal ganglia (TG) change their excitability in several ways at cooling temperatures. In nearly 70% of not-for-cold-sensing TG neurons, the cooling temperature of 15°C increases their membrane excitability. We regard these neurons as cold-active neurons. For the remaining 30% of not-for-cold-sensing TG neurons, the cooling temperature of 15°C either has no effect (regarded as cold-ineffective neurons) or suppress (regarded as cold-suppressive neurons) their membrane excitability. For cold-active neurons, the cold temperature of 15°C increases their excitability as is evidenced by the increases in action potential (AP) firing numbers and/or reduction of AP rheobase when these neurons are depolarized electrically. The cold temperature of 15°C significantly inhibits M-currents and increases membrane input resistance of cold-active neurons. Retigabine, an M-current activator, abolishes the effect of cold temperatures on AP firing but not the effect of cold temperature on AP rheobase levels. The inhibition of M-currents and the increases of membrane input resistance are likely two mechanisms by which cooling temperatures increase the excitability of not-for-cold-sensing TG neurons. PMID:26709732

Effects of cold temperatures on the excitability of rat trigeminal ganglion neurons that are not for cold sensing.

PubMed

Kanda, Hirosato; Gu, Jianguo G

2017-05-01

Aside from a small population of primary afferent neurons for sensing cold, which generate sensations of innocuous and noxious cold, it is generally believed that cold temperatures suppress the excitability of primary afferent neurons not responsible for cold sensing. These not-for-cold-sensing neurons include the majority of non-nociceptive and nociceptive afferent neurons. In this study we have found that the not-for-cold-sensing neurons of rat trigeminal ganglia (TG) change their excitability in several ways at cooling temperatures. In nearly 70% of not-for-cold-sensing TG neurons, a cooling temperature of 15°C increases their membrane excitability. We regard these neurons as cold-active neurons. For the remaining 30% of not-for-cold-sensing TG neurons, the cooling temperature of 15°C either has no effect (cold-ineffective neurons) or suppress their membrane excitability (cold-suppressive neurons). For cold-active neurons, the cold temperature of 15°C increases their excitability as is evidenced by increases in action potential (AP) firing numbers and/or the reduction in AP rheobase when these neurons are depolarized electrically. The cold temperature of 15°C significantly inhibits M-currents and increases membrane input resistance of cold-active neurons. Retigabine, an M-current activator, abolishes the effect of cold temperatures on AP firing, but not the effect of cold temperature on AP rheobase levels. The inhibition of M-currents and the increases of membrane input resistance are likely two mechanisms by which cooling temperatures increase the excitability of not-for-cold-sensing TG neurons. This article is part of the special article series "Pain". © 2015 International Society for Neurochemistry.

Design and validation of the ball-pen probe for measurements in a low-temperature magnetized plasma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bousselin, G.; Cavalier, J.; Pautex, J. F.

Ball-pen probes have been used in fusion devices for direct measurements of the plasma potential. Their application in low-temperature magnetized plasma devices is still subject to studies. In this context, a ball-pen probe has been recently implemented on the linear plasma device Mirabelle. Produced by a thermionic discharge, the plasma is characterized by a low electron temperature and a low density. Plasma confinement is provided by an axial magnetic field that goes up to 100 mT. The principle of the ball-pen probe is to adjust the saturation current ratio to 1 by reducing the electron current contribution. In that case,more » the floating potential of the probe is close to the plasma potential. A thorough study of the ball-pen probe operation is performed for different designs of the probe over a large set of plasma conditions. Comparisons between ball-pen, Langmuir, and emissive probes are conducted in the same plasma conditions. The ball-pen probe is successfully measuring the plasma potential in these specific plasma conditions only if an adapted electronics and an adapted probe size to the plasma characteristic lengths ({lambda}{sub D}, {rho}{sub ce}) are used.« less

Single palindromic molecular beacon-based amplification for genetic analysis of cancers.

PubMed

Li, Feng; Zhao, Hui; Wang, Zheng-Yong; Wu, Zai-Sheng; Yang, Zhe; Li, Cong-Cong; Xu, Huo; Lyu, Jian-Xin; Shen, Zhi-Fa

2017-05-15

The detection of biomarkers is of crucial importance in reducing the morbidity and mortality of complex diseases. Thus, there is a great desire to develop highly efficient and simple sensing methods to fulfill the different diagnostic and therapeutic purposes. Herein, using tumor suppressor p53 gene as model target DNA, we developed a novel palindromic fragment-incorporated molecular beacon (P-MB) that can perform multiple functions, including recognition element, signal reporter, polymerization template and primer. Upon specific hybridization with target DNA, P-MBs can interact with each other and are extended by polymerase without any additional probes. As a result, hybridized targets are peeled off from P-MBs and initiate the next round of reactions, leading to the unique strand displacement amplification (SDA). The newly-proposed enzymatic amplification displays the detection limit as low as 100pM and excellent selectivity in distinguishing single-base mutation with the linear response range from 100pM to 75nM. This is the simplest SDA sensing system so far because of only involving one type of DNA probe. This impressive sensing paradigm is expected to provide new insight into developing new-type of DNA probes that hold tremendous potential with important applications in molecular biology research and clinical diagnosis. Copyright © 2017 Elsevier B.V. All rights reserved.

In situ temperature monitoring in single-molecule FRET experiments

NASA Astrophysics Data System (ADS)

Hartmann, Andreas; Berndt, Frederic; Ollmann, Simon; Krainer, Georg; Schlierf, Michael

2018-03-01

Thermodynamic properties of single molecules including enthalpic and entropic contributions are often determined from experiments by a direct control and precise measurement of the local temperature. However, common temperature monitoring techniques using, for example, ultrafine temperature probes can lead to uncertainties as the probe cannot be placed in the vicinity of the molecule of interest. Here, we devised an approach to measure the local temperature in freely diffusing confocal single-molecule Förster Resonance Energy Transfer (smFRET) experiments in situ by directly adding the temperature-sensitive fluorescent dye Rhodamine B, whose fluorescence lifetime serves as a probe of the local temperature in the confocal volume. We demonstrate that the temperature and FRET efficiencies of static and dynamic molecules can be extracted within one measurement simultaneously, without the need of a reference chamber. We anticipate this technique to be particularly useful in the physicochemical analyses of temperature-dependent biomolecular processes from single-molecule measurements.

40 CFR 89.309 - Analyzers required for gaseous emissions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... probe is below 190 °C, the temperature of the valves, pipework, and so forth, must be controlled so as... probe is above 190 °C, the temperature of the valves, pipework, and so forth, must be controlled so as... items, following the sample probe, in the given order: (A) Pipework, valves, and so forth, controlled so...

40 CFR 89.309 - Analyzers required for gaseous emissions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... probe is below 190 °C, the temperature of the valves, pipework, and so forth, must be controlled so as... probe is above 190 °C, the temperature of the valves, pipework, and so forth, must be controlled so as... items, following the sample probe, in the given order: (A) Pipework, valves, and so forth, controlled so...

Free volume distribution of branched poly(methyl methacrylates): Conformational probes study

NASA Astrophysics Data System (ADS)

Kamalova, D. I.; Remizov, A. B.

2016-12-01

In this work we studied the free volume distribution of the branched poly (methylmethacrylates) by the method of conformational probes. The freezing temperatures of the conformational transitions of the probes introduced into branched polymers were determined by FTIR spectra. The influence of covalently connected fullerene С60 on the freezing temperatures of conformational transitions was shown.

Self-Nulling Lock-in Detection Electronics for Capacitance Probe Electrometer

NASA Technical Reports Server (NTRS)

Blaes, Brent R.; Schaefer, Rembrandt T.

2012-01-01

A multi-channel electrometer voltmeter that employs self-nulling lock-in detection electronics in conjunction with a mechanical resonator with noncontact voltage sensing electrodes has been developed for space-based measurement of an Internal Electrostatic Discharge Monitor (IESDM). The IESDM is new sensor technology targeted for integration into a Space Environmental Monitor (SEM) subsystem used for the characterization and monitoring of deep dielectric charging on spacecraft. Use of an AC-coupled lock-in amplifier with closed-loop sense-signal nulling via generation of an active guard-driving feedback voltage provides the resolution, accuracy, linearity and stability needed for long-term space-based measurement of the IESDM. This implementation relies on adjusting the feedback voltage to drive the sense current received from the resonator s variable-capacitance-probe voltage transducer to approximately zero, as limited by the signal-to-noise performance of the loop electronics. The magnitude of the sense current is proportional to the difference between the input voltage being measured and the feedback voltage, which matches the input voltage when the sense current is zero. High signal-to-noise-ratio (SNR) is achieved by synchronous detection of the sense signal using the correlated reference signal derived from the oscillator circuit that drives the mechanical resonator. The magnitude of the feedback voltage, while the loop is in a settled state with essentially zero sense current, is an accurate estimate of the input voltage being measured. This technique has many beneficial attributes including immunity to drift, high linearity, high SNR from synchronous detection of a single-frequency carrier selected to avoid potentially noisy 1/f low-frequency spectrum of the signal-chain electronics, and high accuracy provided through the benefits of a driven shield encasing the capacitance- probe transducer and guarded input triaxial lead-in. Measurements obtained from a 2- channel prototype electrometer have demonstrated good accuracy (|error| < 0.2 V) and high stability. Twenty-four-hour tests have been performed with virtually no drift. Additionally, 5,500 repeated one-second measurements of 100 V input were shown to be approximately normally distributed with a standard deviation of 140 mV.

Heat recovery system employing a temperature controlled variable speed fan

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jones, W.T.

1986-05-20

A heat recovery system is described for use in recovering heat from an industrial process producing a heated fluid comprising: a source of inlet air; a housing coupled to the source and including a heat exchanger; means for passing the heated fluid through the heat exchanger; the housing including means for moving a variable volume of air adjustable over a continuous range from the source through the heat exchanger; air discharge means communicating with the housing for discharging air which has passed through the heat exchanger; a control system including first temperature sensing means for sensing the discharge temperature ofmore » the discharge air moving through the discharge means and a control circuit coupled to the first temperature sensing means and to the moving means for varying the volume of air moved in response to the sensed discharge temperature to control the temperature of discharge air passing through the discharge means at a first predetermined value; and the control system including second temperature sensing means for sensing the temperature of the source of inlet air and valve means coupled to and controlled by the control circuit to cause liquid to bypass the heat exchanger when the inlet air temperature rises above a second predetermined value.« less

A plastic optical fiber sensor for the dual sensing of temperature and oxygen

NASA Astrophysics Data System (ADS)

Lo, Yu-Lung; Chu, Chen-Shane

2008-04-01

This study presents a low-cost plastic optical fiber sensor for the dual sensing of temperature and oxygen. The sensor features a commercially available epoxy glue coated on the side-polished fiber surface for temperature sensing and a fluorinated xerogel doped with platinum tetrakis pentrafluoropheny porphine (PtTFPP) coated on the fiber end for oxygen sensing. The temperature and oxygen indicators are both excited using a UV LED light source with a wavelength of 380 nm. The luminescence emission spectra of the two indicators are well resolved and exhibit no cross-talk effects. Overall, the results indicate that the dual sensor presented in this study provides an ideal solution for the non-contact, simultaneous sensing of temperature and oxygen in general biological and medical applications.

Upconversion luminescent logic gates and turn-on sensing of glutathione based on two-photon excited quantum dots conjugated with dopamine.

PubMed

Gui, Rijun; Jin, Hui; Liu, Xifeng; Wang, Zonghua; Zhang, Feifei; Xia, Jianfei; Yang, Min; Bi, Sai

2014-12-07

Under the two-photon excitation, upconversion luminescent "INHIBIT" and "OR" logic gates of water-dispersed CdTe quantum dots (QDs) were constituted by conjugating the QDs with dopamine. This facilitated the development of a novel QDs-based upconversion luminescent probe for efficient turn-on sensing of glutathione.

A mechanistic study of hydrogen gas sensing by PdO nanoflake thin films at temperatures below 250 °C.

PubMed

Chiang, Yu-Ju; Li, Kuang-Chung; Lin, Yi-Chieh; Pan, Fu-Ming

2015-02-07

We prepared PdO nanoflake thin films on the SiO2 substrate by reactive sputter deposition, and studied their sensing response to H2 at temperatures between 25 and 250 °C. In addition to the oxygen ionosorption model, which is used to describe the early H2 sensing response over the temperature range studied, the H2 sensing kinetics of the PdO thin films can be separated into three temperature regimes: temperatures below 100 °C, around 150 °C and above 200 °C. At temperatures below 100 °C, PdO reduction is the dominant reaction affecting the H2 sensing behavior. At temperatures around 150 °C, Pd reoxidation kinetically competes with PdO reduction leading to a complicated sensing characteristic. Active PdO reduction by H2 promotes the continuing growth of Pd nanoislands, facilitating dissociative oxygen adsorption and thus the subsequent Pd reoxidation in the H2-dry air gas mixture. The kinetic competition between the PdO reduction and reoxidation at 150 °C leads to the observation of an inverse of the increase in the sensor conductivity. At temperatures above 200 °C, the PdO sensor exhibits a sensor signal monotonically increasing with the H2 concentration, and the H2 sensing behavior is consistent with the Mars-van-Krevelen redox mechanism.

Experimental plasma studies

NASA Technical Reports Server (NTRS)

Dunn, M. G.

1972-01-01

The rate coefficients for the reactions C(+) + e(-) + e(-) yields C + e(-) and CO(+) + e(-) yields C + O were measured over the electron temperature range of approximately 1500 deg K to 7000 deg K. The measurements were performed in CO that had expanded from equilibrium reservoir conditions of 7060 deg K at 17.3 atm pressure and from 6260 deg K at 10.0 atm pressure. Two RAM flight probes were used to measure electron density and electron temperature in the expanding flow of a shock tunnel. Experiments were performed in the inviscid flow with both probes and in the nozzle-wall boundary layer with the constant bias-voltage probe. The distributions of electron density and electron temperature were independently measured using voltage-swept thin-wire probes. Thin-wire Langmuir probes were also used to measure the electron-density and electron-temperature distributions in the boundary layer of a sharp flat plate located on the nozzle centerline. Admittance measurements were performed with the RAM C and RAM C-C S-band antennas in the presence of an ionized boundary layer.

Active radiometer for self-calibrated furnace temperature measurements

DOEpatents

Woskov, P.P.; Cohn, D.R.; Titus, C.H.; Wittle, J.K.; Surma, J.E.

1996-11-12

A radiometer is described with a probe beam superimposed on its field-of-view for furnace temperature measurements. The radiometer includes a heterodyne millimeter/submillimeter-wave receiver including a millimeter/submillimeter-wave source for probing. The receiver is adapted to receive radiation from a surface whose temperature is to be measured. The radiation includes a surface emission portion and a surface reflection portion which includes the probe beam energy reflected from the surface. The surface emission portion is related to the surface temperature and the surface reflection portion is related to the emissivity of the surface. The simultaneous measurement of surface emissivity serves as a real time calibration of the temperature measurement. 5 figs.

Wireless network of stand-alone end effect probes for soil in situ permittivity measurements over the 100MHZ-6GHz frequency range

NASA Astrophysics Data System (ADS)

Demontoux, François; Bircher, Simone; Ruffié, Gilles; Bonnaudiin, Fabrice; Wigneron, Jean-Pierre; Kerr, Yann

2017-04-01

Microwave remote sensing and non-destructive analysis are a powerful way to provide properties estimation of materials. Numerous applications using microwave frequency behavior of materials (remote sensing above land surfaces, non-destructive analysis…) are strongly dependent on the material's permittivity (i.e. dielectric properties). This permittivity depends on numerous parameters such as moisture, texture, temperature, frequency or bulk density. Permittivity measurements are generally carried out in the laboratory. Additionally, dielectric mixing models allow, over a restricted range of conditions, the assessment of a material's permittivity. in-situ measurements are more difficult to obtain. Some in situ measurement probes based on permittivity properties of soil exist (e.g. Time Domain Reflectometers and Transmissometers, capacitance and impedance sensors). They are dedicated to the acquisition of soil moisture data based on permittivity (mainly the real part) estimations over a range of frequencies from around 50 MHz to 1 or 2 GHz. Other Dielectric Assessment Kits exist but they are expensive and they are rather dedicated to laboratory measurements. Furthermore, the user can't address specific issues related to particular materials (e.g. organic soils) or specific measurement conditions (in situ long time records). At the IMS Laboratory we develop probes for in situ soil permittivity measurements (real and imaginary parts) in the 0.5 - 6 GHz frequency range. They are based on the end effect phenomenon of a coaxial waveguide and so are called end effect probes in this paper. The probes can be connected to a portable Vector Network Analyzer (VNA, ANRITSU MS2026A) for the S11 coefficient measurements needed to compute permittivity. It is connected to a PC to record data using an USB connection. This measurement set-up is already used for in situ measurement of soil properties in the framework of the European Space Agency's (ESA) SMOS space mission. However, it should be useful to install many probes on the same site to obtain permittivity measurements over a large area. To reach this goal, the probes should communicate with each other to send data to a record device. Furthermore, it is needed to record measurements over a long time period (many months) to study the in-situ dielectric soil property variations according to changing weather conditions and seasonal trends. The goal of the research work presented is to develop a dielectric sensor system based on end effect probes able to communicate the data using wireless technology. It must be stand-alone from an electric and data recording point of view so it must integrate a VNA circuit instead of the ANRITSU VNA used for the moment. The LoRa wireless technology has been selected because of its low electric consumption and the large distance between equipment available. LoRaWAN™ is a Low Power Wide Area Network specification intended for wireless battery operated devices. The LoRaWAN data rates range from 0.3 kbps to 50 kbps which is sufficient for our probes' data exchanges. We will present the work done to perform the VNA and the LoRa communication board as well as the work done to improve the probes and the permittivity computation algorithm.

Use of Multiple Fluorescent Labels in Biological Sensing

DTIC Science & Technology

2006-05-01

resulting in labels that are brighter and have longer Stokes shifts than the current standard; (B) to make excimer- and exciplex -forming probes for...2) to make excimer- and exciplex -forming probes for repetitive DNA sequences such as telomeres and centromeres, and to demonstrate them both...between fluorophores, and characterized unusual interactions, including water-soluble excimers and exciplexes . We investigated multiple ways to

Copper nanoclusters as probes for turn-on fluorescence sensing of L-lysine.

PubMed

Zhang, Mingming; Qiao, Juan; Zhang, Shufeng; Qi, Li

2018-05-15

Herein, a unique protocol based on copper nanoclusters (CuNCs) probe for turn-on fluorescence sensing of L-lysine was developed. The fluorescent CuNCs with ovalbumin as the stabilizer was prepared by a simple, one-step and green method. When 370 nm was used as the excitation wavelength, the resultant CuNCs exhibited a pale blue fluorescence with the maximum emission at 440 nm. Interestingly, existence of L-lysine evoked the obvious fluorescence intensity increase of CuNCs. The detection limit of the proposed method for L-lysine was 5.5 μM, with a good linear range from 10.0 μM to 1.0 mM (r 2 = 0.999). Moreover, the possible mechanism for enhanced fluorescence intensity of CuNCs by addition of L-lysine was explored and discussed briefly. Further, the as-prepared fluorescent CuNCs was successfully applied in detection of L-lysine in urine. Our results demonstrated that L-lysine could be monitored by the probe, providing new path for construction of CuNCs as fluorescent probes and showing great potential in quantification of L-lysine in real samples. Copyright © 2018 Elsevier B.V. All rights reserved.

Smart Sensing Methodology for Object Identification Using Circularly Polarized Luminescence from Coordination-Driven Self-Assembly.

PubMed

Imai, Yuki; Nakano, Yuka; Kawai, Tsuyoshi; Yuasa, Junpei

2018-05-21

This work demonstrates a potential use of circularly polarized luminescence for object identification methodology in a sensor application. Towards this aim, we have developed new luminescence probes using pyrene derivatives as sensor luminophores. The probes [(R,R)- and (S,S)-Im2Py] contain two chiral imidazole moieties at 1,6-positions through ethynyl spacers (the angle between the spacers is close to 180°). The probe molecules spontaneously self-assemble into chiral stacks (P or M helicity) upon coordination to metal ions with tetrahedral coordination preference (e.g., Zn2+). The chiral probes display neither circular dichroism (CD) nor circularly polarized luminescence (CPL) in the absence of metal ions. However, [(R,R)- and (S,S)-Im2Py] begins to exhibit intense chiroptical activity (CD and CPL) upon self-assembly with Zn2+ ions. The unique chiroptical properties of [(R,R)- and (S,S)-Im2Py] with chemical stimuli-responsibility are capable of demonstrating the new sensing methodology using the CPL signal as detection output, enabling us to discriminate between a signal from the target analyte and that from non-target species. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Covert laser remote sensing and vibrometry

NASA Technical Reports Server (NTRS)

Maleki, Lutfollah (Inventor); Yu, Nan (Inventor); Matsko, Andrey B. (Inventor); Savchenkov, Anatoliy (Inventor)

2012-01-01

Designs of single-beam laser vibrometry systems and methods. For example, a method for detecting vibrations of a target based on optical sensing is provided to include operating a laser to produce a laser probe beam at a laser frequency and modulated at a modulation frequency onto a target; collecting light at or near the laser to collect light from the target while the target is being illuminated by the laser probe beam through an optical receiver aperture; using a narrow-band optical filter centered at the laser frequency to filter light collected from the optical receiver aperture to transmit light at the laser frequency while blocking light at other frequencies; using an optical detector to convert filtered light from the narrow-band optical filter to produce a receiver electrical signal; using a lock-in amplifier to detect and amplify the receiver electrical signal at the modulation frequency while rejecting signal components at other frequencies to produce an amplified receiver electrical signal; processing the amplified receiver electrical signal to extract information on vibrations of the target carried by reflected laser probe beam in the collected light; and controlling optical power of the laser probe beam at the target to follow optical power of background illumination at the target.

Comparison of Cherenkov excited fluorescence and phosphorescence molecular sensing from tissue with external beam irradiation.

PubMed

Lin, Huiyun; Zhang, Rongxiao; Gunn, Jason R; Esipova, Tatiana V; Vinogradov, Sergei; Gladstone, David J; Jarvis, Lesley A; Pogue, Brian W

2016-05-21

Ionizing radiation delivered by a medical linear accelerator (LINAC) generates Cherenkov emission within the treated tissue. A fraction of this light, in the 600-900 nm wavelength region, propagates through centimeters of tissue and can be used to excite optical probes in vivo, enabling molecular sensing of tissue analytes. The success of isolating the emission signal from this Cherenkov excitation background is dependent on key factors such as: (i) the Stokes shift of the probe spectra; (ii) the excited state lifetime; (iii) the probe concentration; (iv) the depth below the tissue surface; and (v) the radiation dose used. Previous studies have exclusively focused on imaging phosphorescent dyes, rather than fluorescent dyes. However there are only a few biologically important phosphorescent dyes and yet in comparison there are thousands of biologically relevant fluorescent dyes. So in this study the focus was a study of efficacy of Cherenkov-excited luminescence using fluorescent commercial near-infrared probes, IRDye 680RD, IRDye 700DX, and IRDye 800CW, and comparing them to the well characterized phosphorescent probe Oxyphor PtG4, an oxygen sensitive dye. Each probe was excited by Cherenkov light from a 6 MV external radiation beam, and measured in continuous wave or time-gated modes. The detection was performed by spectrally resolving the luminescence signals, and measuring them with spectrometer-based separation on an ICCD detector. The results demonstrate that IRDye 700DX and PtG4 allowed for the maximal signal to noise ratio. In the case of the phosphorescent probe, PtG4, with emission decays on the microsecond (μs) time scale, time-gated acquisition was possible, and it allowed for higher efficacy in terms of the probe concentration and detection depth. Phantoms containing the probe at 5 mm depth could be detected at concentrations down to the nanoMolar range, and at depths into the tissue simulating phantom near 3 cm. In vivo studies showed that 5 nmol of dye was readily detected with radiation doses less than 5 cGy. Since concentration, radiation dose and depth each contribute to the level of the detected signal, it may be possible to improve any of these parameters at expense of the others. This paradigm of nanoMolar sensitivity for optical reporters in vivo introduces the concept of molecular sensing of tumors during therapy or diagnostically with biologically relevant concentrations of fluorescent reporters.

Comparison of Cherenkov excited fluorescence and phosphorescence molecular sensing from tissue with external beam irradiation

NASA Astrophysics Data System (ADS)

Lin, Huiyun; Zhang, Rongxiao; Gunn, Jason R.; Esipova, Tatiana V.; Vinogradov, Sergei; Gladstone, David J.; Jarvis, Lesley A.; Pogue, Brian W.

2016-05-01

Ionizing radiation delivered by a medical linear accelerator (LINAC) generates Cherenkov emission within the treated tissue. A fraction of this light, in the 600-900 nm wavelength region, propagates through centimeters of tissue and can be used to excite optical probes in vivo, enabling molecular sensing of tissue analytes. The success of isolating the emission signal from this Cherenkov excitation background is dependent on key factors such as: (i) the Stokes shift of the probe spectra; (ii) the excited state lifetime; (iii) the probe concentration; (iv) the depth below the tissue surface; and (v) the radiation dose used. Previous studies have exclusively focused on imaging phosphorescent dyes, rather than fluorescent dyes. However there are only a few biologically important phosphorescent dyes and yet in comparison there are thousands of biologically relevant fluorescent dyes. So in this study the focus was a study of efficacy of Cherenkov-excited luminescence using fluorescent commercial near-infrared probes, IRDye 680RD, IRDye 700DX, and IRDye 800CW, and comparing them to the well characterized phosphorescent probe Oxyphor PtG4, an oxygen sensitive dye. Each probe was excited by Cherenkov light from a 6 MV external radiation beam, and measured in continuous wave or time-gated modes. The detection was performed by spectrally resolving the luminescence signals, and measuring them with spectrometer-based separation on an ICCD detector. The results demonstrate that IRDye 700DX and PtG4 allowed for the maximal signal to noise ratio. In the case of the phosphorescent probe, PtG4, with emission decays on the microsecond (μs) time scale, time-gated acquisition was possible, and it allowed for higher efficacy in terms of the probe concentration and detection depth. Phantoms containing the probe at 5 mm depth could be detected at concentrations down to the nanoMolar range, and at depths into the tissue simulating phantom near 3 cm. In vivo studies showed that 5 nmol of dye was readily detected with radiation doses less than 5 cGy. Since concentration, radiation dose and depth each contribute to the level of the detected signal, it may be possible to improve any of these parameters at expense of the others. This paradigm of nanoMolar sensitivity for optical reporters in vivo introduces the concept of molecular sensing of tumors during therapy or diagnostically with biologically relevant concentrations of fluorescent reporters.

Optical Probes for Neurobiological Sensing and Imaging.

PubMed

Kim, Eric H; Chin, Gregory; Rong, Guoxin; Poskanzer, Kira E; Clark, Heather A

2018-05-15

Fluorescent nanosensors and molecular probes are next-generation tools for imaging chemical signaling inside and between cells. Electrophysiology has long been considered the gold standard in elucidating neural dynamics with high temporal resolution and precision, particularly on the single-cell level. However, electrode-based techniques face challenges in illuminating the specific chemicals involved in neural cell activation with adequate spatial information. Measuring chemical dynamics is of fundamental importance to better understand synergistic interactions between neurons as well as interactions between neurons and non-neuronal cells. Over the past decade, significant technological advances in optical probes and imaging methods have enabled entirely new possibilities for studying neural cells and circuits at the chemical level. These optical imaging modalities have shown promise for combining chemical, temporal, and spatial information. This potential makes them ideal candidates to unravel the complex neural interactions at multiple scales in the brain, which could be complemented by traditional electrophysiological methods to obtain a full spatiotemporal picture of neurochemical dynamics. Despite the potential, only a handful of probe candidates have been utilized to provide detailed chemical information in the brain. To date, most live imaging and chemical mapping studies rely on fluorescent molecular indicators to report intracellular calcium (Ca 2+ ) dynamics, which correlates with neuronal activity. Methodological advances for monitoring a full array of chemicals in the brain with improved spatial, temporal, and chemical resolution will thus enable mapping of neurochemical circuits with finer precision. On the basis of numerous studies in this exciting field, we review the current efforts to develop and apply a palette of optical probes and nanosensors for chemical sensing in the brain. There is a strong impetus to further develop technologies capable of probing entire neurobiological units with high spatiotemporal resolution. Thus, we introduce selected applications for ion and neurotransmitter detection to investigate both neurons and non-neuronal brain cells. We focus on families of optical probes because of their ability to sense a wide array of molecules and convey spatial information with minimal damage to tissue. We start with a discussion of currently available molecular probes, highlight recent advances in genetically modified fluorescent probes for ions and small molecules, and end with the latest research in nanosensors for biological imaging. Customizable, nanoscale optical sensors that accurately and dynamically monitor the local environment with high spatiotemporal resolution could lead to not only new insights into the function of all cell types but also a broader understanding of how diverse neural signaling systems act in conjunction with neighboring cells in a spatially relevant manner.

Compensation of Verdet Constant Temperature Dependence by Crystal Core Temperature Measurement

PubMed Central

Petricevic, Slobodan J.; Mihailovic, Pedja M.

2016-01-01

Compensation of the temperature dependence of the Verdet constant in a polarimetric extrinsic Faraday sensor is of major importance for applying the magneto-optical effect to AC current measurements and magnetic field sensing. This paper presents a method for compensating the temperature effect on the Faraday rotation in a Bi12GeO20 crystal by sensing its optical activity effect on the polarization of a light beam. The method measures the temperature of the same volume of crystal that effects the beam polarization in a magnetic field or current sensing process. This eliminates the effect of temperature difference found in other indirect temperature compensation methods, thus allowing more accurate temperature compensation for the temperature dependence of the Verdet constant. The method does not require additional changes to an existing Δ/Σ configuration and is thus applicable for improving the performance of existing sensing devices. PMID:27706043

Flexible and self-powered temperature-pressure dual-parameter sensors using microstructure-frame-supported organic thermoelectric materials.

PubMed

Zhang, Fengjiao; Zang, Yaping; Huang, Dazhen; Di, Chong-an; Zhu, Daoben

2015-09-21

Skin-like temperature- and pressure-sensing capabilities are essential features for the next generation of artificial intelligent products. Previous studies of e-skin and smart elements have focused on flexible pressure sensors, whereas the simultaneous and sensitive detection of temperature and pressure with a single device remains a challenge. Here we report developing flexible dual-parameter temperature-pressure sensors based on microstructure-frame-supported organic thermoelectric (MFSOTE) materials. The effective transduction of temperature and pressure stimuli into two independent electrical signals permits the instantaneous sensing of temperature and pressure with an accurate temperature resolution of <0.1 K and a high-pressure-sensing sensitivity of up to 28.9 kPa(-1). More importantly, these dual-parameter sensors can be self-powered with outstanding sensing performance. The excellent sensing properties of MFSOTE-based devices, together with their unique advantages of low cost and large-area fabrication, make MFSOTE materials possess promising applications in e-skin and health-monitoring elements.

Flexible and self-powered temperature-pressure dual-parameter sensors using microstructure-frame-supported organic thermoelectric materials

NASA Astrophysics Data System (ADS)

Zhang, Fengjiao; Zang, Yaping; Huang, Dazhen; di, Chong-An; Zhu, Daoben

2015-09-01

Skin-like temperature- and pressure-sensing capabilities are essential features for the next generation of artificial intelligent products. Previous studies of e-skin and smart elements have focused on flexible pressure sensors, whereas the simultaneous and sensitive detection of temperature and pressure with a single device remains a challenge. Here we report developing flexible dual-parameter temperature-pressure sensors based on microstructure-frame-supported organic thermoelectric (MFSOTE) materials. The effective transduction of temperature and pressure stimuli into two independent electrical signals permits the instantaneous sensing of temperature and pressure with an accurate temperature resolution of <0.1 K and a high-pressure-sensing sensitivity of up to 28.9 kPa-1. More importantly, these dual-parameter sensors can be self-powered with outstanding sensing performance. The excellent sensing properties of MFSOTE-based devices, together with their unique advantages of low cost and large-area fabrication, make MFSOTE materials possess promising applications in e-skin and health-monitoring elements.

Development of nanowire arrays for neural probe

NASA Astrophysics Data System (ADS)

Abraham, Jose K.; Xie, Jining; Varadan, Vijay K.

2005-05-01

It is already established that functional electrical stimulation is an effective way to restore many functions of the brain in disabled individuals. The electrical stimulation can be done by using an array of electrodes. Neural probes stimulate or sense the biopotentials mainly through the exposed metal sites. These sites should be smaller relative to the spatial potential distribution so that any potential averaging in the sensing area can be avoided. At the same time, the decrease in size of these sensing sites is limited due to the increase in impedance levels and the thermal noise while decreasing its size. It is known that current density in a planar electrode is not uniform and a higher current density can be observer around the perimeter of the electrodes. Electrical measurements conducted on many nanotubes and nanowires have already proved that it could be possible to use for current density applications and the drawbacks of the present design in neural probes can be overcome by incorporating many nanotechnology solutions. In this paper we present the design and development of nanowire arrays for the neural probe for the multisite contact which has the ability to collect and analyze isolated single unit activity. An array of vertically grown nanowires is used as contact site and many of such arrays can be used for stimulating as well as recording sites. The nanolevel interaction and wireless communication solution can extend to applications involving the treatment of many neurological disorders including Parkinson"s disease, Alzheimer"s disease, spinal injuries and the treatment of blindness and paralyzed patients with minimal or no invasive surgical procedures.

Spectra of cosmic X-ray sources

NASA Technical Reports Server (NTRS)

Holt, S. S.; Mccray, R.

1982-01-01

X-ray measurements provide the most direct probes of astrophysical environments with temperatures exceeding one million K. Progress in experimental research utilizing dispersive techniques (e.g., Bragg and grating spectroscopy) is considerably slower than that in areas utilizing photometric techniques, because of the relative inefficiency of the former for the weak X-ray signals from celestial sources. As a result, the term "spectroscopy" as applied to X-ray astronomy has traditionally satisfied a much less restrictive definition (in terms of resolving power) than it has in other wavebands. Until quite recently, resolving powers of order unity were perfectly respectable, and still provide (in most cases) the most useful spectroscopic data. In the broadest sense, X-ray photometric measurements are spectroscopic, insofar as they represent samples of the overall electromagnetic continua of celestial objects.

Investigation on Two Forms of Temperature-Sensing Parameters for Fluorescence Intensity Ratio Thermometry Based on Thermal Coupled Theory.

PubMed

Jia, Mochen; Liu, Guofeng; Sun, Zhen; Fu, Zuoling; Xu, Weiguo

2018-02-05

Absolute temperature sensitivity (S a ) reflects the precision of sensors that belong to the same mechanism, whereas relative temperature sensitivity (S r ) is used to compare sensors from different mechanisms. For the fluorescence intensity ratio (FIR) thermometry based on two thermally coupled energy levels of one rare earth (RE) ion, we define a new ratio as the temperature-sensing parameter that can vary greatly with temperature in some circumstances, which can obtain higher S a without changing S r . Further discussion is made on the conditions under which these two forms of temperature-sensing parameters can be used to achieve higher S a for biomedical temperature sensing. Based on the new ratio as the temperature-sensing parameter, the S a and S r of the BaTiO 3 : 0.01%Pr 3+ , 8%Yb 3+ nanoparticles at 313 K reach as high as 0.1380 K -1 and 1.23% K -1 , respectively. Similarly, the S a and S r of the BaTiO 3 : 1%Er 3+ , 3%Yb 3+ nanoparticles at 313 K are as high as 0.0413 K -1 and 1.05% K -1 , respectively. By flexibly choosing the two ratios as the temperature-sensing parameter, higher S a can be obtained at the target temperature, which means higher precision for the FIR thermometers.

Gas-Sensing Characteristics of SrFeO3-δ Thin Film Probed by a Homemade Apparatus

NASA Astrophysics Data System (ADS)

Manikandan, M.; Santhosh Kumar, B.; Mukil Raj, T.; Moorthy Babu, S.; Venkateswaran, C.

2018-05-01

SrFeO2.97 phase obtained by annealing radiofrequency-sputtered stoichiometric film at 600°C for 24 h has been tested using a homemade apparatus coupled with impedance spectrometry. Oxygen nonstoichiometry plays a vital role in sensing of reducing gases. Hence, this phase showed efficient sensing performance for different reducing gases such as acetone (CH3COCH3), ethanol (C2H5OH), ammonia (NH3), and hydrogen sulfide (H2S), with high sensing response recorded for H2S gas at 60°C.

Scientific Rationale of a Saturn Probe Mission

NASA Astrophysics Data System (ADS)

Mousis, Olivier; Fletcher, Leigh N.; Lebreton, Jean-Pierre; Wurz, Peter; Cavalié, Thibault; Coustenis, Athena; Atkinson, Dave H.; Atreya, Sushil; Gautier, Daniel; Guillot, Tristan; Lunine, Jonathan I.; Marty, Bernard; Morse, Andrew D.; Rey, Kim R.; Simon-Miller, Amy; Spilker, Thomas R.; Waite, Jack Hunter

2014-05-01

Remote sensing observations meet some limitations when used to study the bulk atmospheric composition of the giant planets of our solar system. A remarkable example of the unicity of in situ probe measurements is illustrated by the exploration of Jupiter, where key measurements such as noble gases abundances and the precise measurement of the helium mixing ratio have only been made available through in situ measurements by the Galileo probe. Here we describe the main scientific goals to be addressed by future in situ exploration of Saturn. Planet formation: To understand the formation of giant planets and the origin of our Solar System, statistical data obtained from the observation of exoplanetary systems must be supplemented by direct measurements of the composition of the planets in our solar system. A giant planet's bulk composition depends on the timing and location of planet formation, subsequent migration and the delivery mechanisms for the heavier elements. By measuring a giant planet's chemical inventory, and contrasting these with measurements of (i) other giant planets, (ii) primitive materials found in small bodies, and (iii) the composition of our parent star and the local interstellar medium, much can be revealed about the conditions at work during the formation of our planetary system [1]. To date, the Galileo probe at Jupiter (1995) remains our only data point for interpreting the bulk composi-tion of the giant planets. Galileo found that Jupiter exhibited an enrichment in C, N, S, Ar, Kr and Xe compared to the solar photospheric abundances, with some notable exceptions - water was found depleted, possibly due to meteorological processes at the probe entry site; and neon was also found depleted, possibly due to rain-out to deeper levels [2]. Explaining the high abundance of noble gases requires either condensing these elements directly at low-temperature in the form of amorphous ices [3], trapping them as clathrates [4-7] or photoevaporating the hydrogen and helium in the protoplanetary disk during the planet's formation [8]. The in situ Galileo measurements at Jupiter also include a highly precise determination of the planet's helium abundance, crucial for studies of the structure and evolution of the planet. Because of the lack of in situ measurements, Saturn noble gas abundances are unknown and their determi-nation is missing to properly understand its formation conditions. There is however some indication for a non-uniform enrichment in C, N and S. [5] suggests that observations are well fitted if the atmospheric C and N of the planet were initially mainly in reduced forms at 10 AU in the protosolar nebula. Alternatively, [6] finds that it is possible to account for these enrich-ments in a way consistent with those measured at Jupi-ter if the building blocks of the two planets shared a common origin. As in Jupiter, the missing piece of the puzzle remains the measurement of the oxygen abundance. Precisely measuring in situ the He/H2 ratio in Saturn is also needed for properly modeling its interior and thermal evolution. Planetary Atmospheric Processes: Saturn's complex and cloud-dominated weather-layer is our principle gateway to the processes at work within the deep interior of this giant planet. We must extrapolate from this thin, dynamic region over many orders of magnitude in pressure, temperature and density to infer the planetary properties deep below the clouds [1]. Remote sensing provides insights into the complexity of the transitional zone between the external environment and the fluid interior, but there is much that we still do not under-stand. In situ measurements are the only method providing ground-truth to connect the remote sensing inferences with physical reality, and yet this has only been achieved twice in the history of outer solar system exploration, via the Galileo probe for Jupiter and the Huygens probe for Titan. In situ studies provide access to atmospheric regions that are beyond the reach of remote sensing, enabling us to study the dynamical, chemical and aerosol-forming processes at work from the thermosphere to the troposphere below the cloud decks. Two crucial questions in this theme remain i) the nature of the processes at work in planetary atmospheres, shaping the dynamics and circulation from the thermosphere to the deep troposphere (e.g., [9]) , and ii) the chemical properties and conditions for cloud formation as a function of depth and temperature in planetary atmospheres (e.g., [10]). References: [1] Mousis O. et al. (2014) PSS, submit-ted. [2] Niemann H. B. et al. (1998) JGR, 103, 22831-22846. [3] Owen T. et al. (1999) Nature, 402, 269-270. [4] Gautier D. et al. (2001) ApJ, 550, L227-L230. [5] Hersant F. et al. (2008) PSS, 56, 1103-1111. [6] Mousis O. et al. (2009) ApJ, 696, 1348-1354. [7] Mousis et al. (2012) ApJ, 751, L7. [8] Guillot T. and Hueso R. (2006) MNRAS, 367, L47-L51. [9] Del Gen-io et al., (2009) Saturn after Cassini-Huygens, Ch. 6, pp. 113-159. [10] West et al., (2009), Saturn after Cas-sini-Huygens, Ch. 7, pp. 161-179. [11] Seiff et al., (1998), JGR, 103, 22857-22890 [12] Atkinson et al., JGR, 103, 22911-22928 (1998). [13] Wong et al., (2004), Icarus 171, 153-170. [14] Fouchet et al., Sat-urn after Cassini-Huygens, Ch. 5, pp. 83-112 (2009).

U-shaped, double-tapered, fiber-optic sensor for effective biofilm growth monitoring.

PubMed

Zhong, Nianbing; Zhao, Mingfu; Li, Yishan

2016-02-01

To monitor biofilm growth on polydimethylsiloxane in a photobioreactor effectively, the biofilm cells and liquids were separated and measured using a sensor with two U-shaped, double-tapered, fiber-optic probes (Sen. and Ref. probes). The probes' Au-coated hemispherical tips enabled double-pass evanescent field absorption. The Sen. probe sensed the cells and liquids inside the biofilm. The polyimide-silica hybrid-film-coated Ref. probe separated the liquids from the biofilm cells and analyzed the liquid concentration. The biofilm structure and active biomass were also examined to confirm the effectiveness of the measurement using a simulation model. The sensor was found to effectively respond to the biofilm growth in the adsorption through exponential phases at thicknesses of 0-536 μm.

Spatially controlled immobilisation of biomolecules: A complete approach in green chemistry

NASA Astrophysics Data System (ADS)

Grinenval, Eva; Nonglaton, Guillaume; Vinet, Françoise

2014-01-01

The development of 'green' sensors is a challenging task in the field of biomolecule sensing, for example in the detection of cardiac troponin-I (cTnI). In the present work a complete approach in green chemistry was developed to create chemically active patterns for the immobilisation of biological probes. This key technology is discussed on the basis of the twelve green chemistry principles, and is a combination of surface patterning by spotting and surface chemistries modified by molecular vapour deposition. The (1H,1H,2H,2H)-perfluorodecyltrichlorosilane (FDTS) was used as a novel anti-adsorption layer while the 3,4-epoxybutyltrimethoxysilane (EBTMOS) was used to immobilise probes. Oligonucleotides and the anti-cTnI antibody were studied. The spatially controlled immobilisation of probes was characterised by fluorescence. The demonstrated surface modification has broad applications in areas such as diagnostics and bio-chemical sensing. Moreover, the environmental impacts of surface patterning and surface chemistry were discussed from a 'greenness' point of view.

Infrared Fiber Optic Sensors

NASA Technical Reports Server (NTRS)

1997-01-01

Successive years of Small Business Innovation Research (SBIR) contracts from Langley Research Center to Sensiv Inc., a joint venture between Foster-Miller Inc. and Isorad, Ltd., assisted in the creation of remote fiber optic sensing systems. NASA's SBIR interest in infrared, fiber optic sensor technology was geared to monitoring the curing cycles of advanced composite materials. These funds helped in the fabrication of an infrared, fiber optic sensor to track the molecular vibrational characteristics of a composite part while it is being cured. Foster-Miller ingenuity allowed infrared transmitting optical fibers to combine with Fourier Transform Infrared spectroscopy to enable remote sensing. Sensiv probes operate in the mid-infrared range of the spectrum, although modifications to the instrument also permits its use in the near-infrared region. The Sensiv needle-probe is built to be placed in a liquid or powder and analyze the chemicals in the mixture. Other applications of the probe system include food processing control; combustion control in furnaces; and maintenance problem solving.

A turn-on supramolecular fluorescent probe for sensing benzimidazole fungicides and its application in living cell imaging

NASA Astrophysics Data System (ADS)

Tang, Qing; Zhang, Jing; Sun, Tao; Wang, Cheng-Hui; Huang, Ying; Zhou, Qingdi; Wei, Gang

2018-02-01

A cucurbit[8]uril-based turn-on supramolecular fluorescent probe between cucurbit[8]uril (Q[8]) and pyronine Y (PyY) (designated 2PyY@Q[8]) in acidic aqueous solution showed a remarkable fluorescence 'turn-on' response to benzimidazole fungicides such as thiabendazole, fuberidazole and carbendazim. The 2PyY@Q[8] fluorescent probe can be used to detect benzimidazole fungicides with high sensitivity and selectivity with a detection limit of 10- 8 mol/L. A good linear relationship of emission intensity at 580 nm for benzimidazole fungicides at concentrations of 0.4-5.0 μmol/L was observed. The proposed sensing mechanism was investigated using 1H NMR spectroscopy combined with density functional theory calculations at the B3LYP/6-31G(d) level. The cell imaging study showed that the 2PyY@Q[8] complex could be used to image benzimidazole fungicide in prostate cancer (PC3) cells, which may help to elucidate relevant biological processes at the molecular level.

Detonation wave detection probe including parallel electrodes on a flexible backing strip

DOEpatents

Uher, K.J.

1995-12-19

A device is disclosed for sensing the occurrence of destructive events and events involving mechanical shock in a non-intrusive manner. A pair of electrodes is disposed in a parallel configuration on a backing strip of flexible film. Electrical circuitry is used to sense the time at which an event causes electrical continuity between the electrodes or, with a sensor configuration where the electrodes are shorted together, to sense the time at which electrical continuity is lost. 4 figs.

Global versus local mechanisms of temperature sensing in ion channels.

PubMed

Arrigoni, Cristina; Minor, Daniel L

2018-05-01

Ion channels turn diverse types of inputs, ranging from neurotransmitters to physical forces, into electrical signals. Channel responses to ligands generally rely on binding to discrete sensor domains that are coupled to the portion of the channel responsible for ion permeation. By contrast, sensing physical cues such as voltage, pressure, and temperature arises from more varied mechanisms. Voltage is commonly sensed by a local, domain-based strategy, whereas the predominant paradigm for pressure sensing employs a global response in channel structure to membrane tension changes. Temperature sensing has been the most challenging response to understand and whether discrete sensor domains exist for pressure and temperature has been the subject of much investigation and debate. Recent exciting advances have uncovered discrete sensor modules for pressure and temperature in force-sensitive and thermal-sensitive ion channels, respectively. In particular, characterization of bacterial voltage-gated sodium channel (BacNa V ) thermal responses has identified a coiled-coil thermosensor that controls channel function through a temperature-dependent unfolding event. This coiled-coil thermosensor blueprint recurs in other temperature sensitive ion channels and thermosensitive proteins. Together with the identification of ion channel pressure sensing domains, these examples demonstrate that "local" domain-based solutions for sensing force and temperature exist and highlight the diversity of both global and local strategies that channels use to sense physical inputs. The modular nature of these newly discovered physical signal sensors provides opportunities to engineer novel pressure-sensitive and thermosensitive proteins and raises new questions about how such modular sensors may have evolved and empowered ion channel pores with new sensibilities.

Floating Probe Assembly for Measuring Temperature of Water

NASA Technical Reports Server (NTRS)

Stewart, Randy; Ruffin, Clyde

2002-01-01

A floating apparatus denoted a temperature probe aquatic suspension system (TPASS) has been developed for measuring the temperature of an ocean, lake, or other natural body of water at predetermined depths. Prior instruments built for the same purpose were found to give inaccurate readings because the apparatuses themselves significantly affected the temperatures of the water in their vicinities. The design of the TPASS is intended to satisfy a requirement to minimize the perturbation of the temperatures to be measured. The TPASS includes a square-cross-section aluminum rod 28 in. (=71 cm) long with floats attached at both ends. Each float includes five polystyrene foam disks about 3/4 in.(=1.9 cm) thick and 2.5 in. (=6.4 cm) in diameter. The disks are stacked to form cylinders, bolted to the rod, and covered with hollow plastic sleeves. A metal sleeve is clamped to the middle of the aluminum rod, from whence it hangs down into the water. Temperature probes (which can be thermocouples, thermistors, or resistance temperature devices) are placed within the sleeve at the desired measurement depths. Wires from the temperature probes are routed to the input terminals of a data logger.

Floating Probe Assembly for Measuring Temperature of Water

NASA Technical Reports Server (NTRS)

Selinsky, T.; Stewart, Randy; Ruffin, Clyde

2002-01-01

A floating apparatus denoted a temperature probe aquatic suspension system (TPASS) has been developed for measuring the temperature of an ocean, lake, or other natural body of water at predetermined depths. Prior instruments built for the same purpose were found to give inaccurate readings because the apparatuses themselves significantly affected the temperatures of the water in their vicinities. The design of the TPASS is intended to satisfy a requirement to minimize the perturbation of the temperatures to be measured. The TPASS includes a square-cross-section aluminum rod 28 in. (approx. = 71 cm) long with floats attached at both ends. Each float includes five polystyrene foam disks about 3/4 in. (approx. = 1.9 cm) thick and 2.5 in. (approx. = 6.4 cm) in diameter. The disks are stacked to form cylinders, bolted to the rod, and covered with hollow plastic sleeves. A metal sleeve is clamped to the middle of the aluminum rod, from whence it hangs down into the water. Temperature probes (which can be thermocouples, thermistors, or resistance temperature devices) are placed within the sleeve at the desired measurement depths. Wires from the temperature probes are routed to the input terminals of a data logger. This work was done by Randy