45 CFR 1210.3-8 - Termination file and Examiner's report.

Code of Federal Regulations, 2011 CFR

2011-10-01

... NATIONAL AND COMMUNITY SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-8 Termination file and Examiner's report. (a) Preparation and Content... part of the termination file. (b) Review by Volunteer. On completion of the termination file, the...

45 CFR 1210.3-8 - Termination file and Examiner's report.

Code of Federal Regulations, 2010 CFR

2010-10-01

... NATIONAL AND COMMUNITY SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-8 Termination file and Examiner's report. (a) Preparation and Content... part of the termination file. (b) Review by Volunteer. On completion of the termination file, the...

45 CFR 1210.3-8 - Termination file and Examiner's report.

Code of Federal Regulations, 2013 CFR

2013-10-01

... NATIONAL AND COMMUNITY SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-8 Termination file and Examiner's report. (a) Preparation and Content... part of the termination file. (b) Review by Volunteer. On completion of the termination file, the...

78 FR 17890 - Energy Efficiency Program for Commercial and Industrial Equipment: Public Meeting and...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-25

... Document for Packaged Terminal Air Conditioners and Packaged Terminal Heat Pumps AGENCY: Office of Energy... must identify the framework document for packaged terminal air conditioners and packaged terminal heat... packaged terminal air conditioners and packaged terminal heat pumps. 78 FR 12252. The document provided for...

7 CFR 1469.25 - Contract violations and termination.

Code of Federal Regulations, 2010 CFR

2010-01-01

... termination without delay. (c) If NRCS terminates a contract due to breach of contract, the participant will... terminates a contract due to breach of contract, or the participant voluntarily terminates the contract... 7 Agriculture 10 2010-01-01 2010-01-01 false Contract violations and termination. 1469.25 Section...

29 CFR 4041.42 - Administration of plan during termination process.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 9 2010-07-01 2010-07-01 false Administration of plan during termination process. 4041.42... TERMINATIONS TERMINATION OF SINGLE-EMPLOYER PLANS Distress Termination Process § 4041.42 Administration of plan during termination process. (a) General rule. Except to the extent specifically prohibited by this...

Trigger loop dynamics can explain stimulation of intrinsic termination by bacterial RNA polymerase without terminator hairpin contact.

PubMed

Ray-Soni, Ananya; Mooney, Rachel A; Landick, Robert

2017-10-31

In bacteria, intrinsic termination signals cause disassembly of the highly stable elongating transcription complex (EC) over windows of two to three nucleotides after kilobases of RNA synthesis. Intrinsic termination is caused by the formation of a nascent RNA hairpin adjacent to a weak RNA-DNA hybrid within RNA polymerase (RNAP). Although the contributions of RNA and DNA sequences to termination are largely understood, the roles of conformational changes in RNAP are less well described. The polymorphous trigger loop (TL), which folds into the trigger helices to promote nucleotide addition, also is proposed to drive termination by folding into the trigger helices and contacting the terminator hairpin after invasion of the hairpin in the RNAP main cleft [Epshtein V, Cardinale CJ, Ruckenstein AE, Borukhov S, Nudler E (2007) Mol Cell 28:991-1001]. To investigate the contribution of the TL to intrinsic termination, we developed a kinetic assay that distinguishes effects of TL alterations on the rate at which ECs terminate from effects of the TL on the nucleotide addition rate that indirectly affect termination efficiency by altering the time window in which termination can occur. We confirmed that the TL stimulates termination rate, but found that stabilizing either the folded or unfolded TL conformation decreased termination rate. We propose that conformational fluctuations of the TL (TL dynamics), not TL-hairpin contact, aid termination by increasing EC conformational diversity and thus access to favorable termination pathways. We also report that the TL and the TL sequence insertion (SI3) increase overall termination efficiency by stimulating pausing, which increases the flux of ECs into the termination pathway. Published under the PNAS license.

Trigger loop dynamics can explain stimulation of intrinsic termination by bacterial RNA polymerase without terminator hairpin contact

PubMed Central

Ray-Soni, Ananya; Mooney, Rachel A.; Landick, Robert

2017-01-01

In bacteria, intrinsic termination signals cause disassembly of the highly stable elongating transcription complex (EC) over windows of two to three nucleotides after kilobases of RNA synthesis. Intrinsic termination is caused by the formation of a nascent RNA hairpin adjacent to a weak RNA−DNA hybrid within RNA polymerase (RNAP). Although the contributions of RNA and DNA sequences to termination are largely understood, the roles of conformational changes in RNAP are less well described. The polymorphous trigger loop (TL), which folds into the trigger helices to promote nucleotide addition, also is proposed to drive termination by folding into the trigger helices and contacting the terminator hairpin after invasion of the hairpin in the RNAP main cleft [Epshtein V, Cardinale CJ, Ruckenstein AE, Borukhov S, Nudler E (2007) Mol Cell 28:991–1001]. To investigate the contribution of the TL to intrinsic termination, we developed a kinetic assay that distinguishes effects of TL alterations on the rate at which ECs terminate from effects of the TL on the nucleotide addition rate that indirectly affect termination efficiency by altering the time window in which termination can occur. We confirmed that the TL stimulates termination rate, but found that stabilizing either the folded or unfolded TL conformation decreased termination rate. We propose that conformational fluctuations of the TL (TL dynamics), not TL-hairpin contact, aid termination by increasing EC conformational diversity and thus access to favorable termination pathways. We also report that the TL and the TL sequence insertion (SI3) increase overall termination efficiency by stimulating pausing, which increases the flux of ECs into the termination pathway. PMID:29078293

Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

PubMed Central

Liskova, Jana; Babchenko, Oleg; Varga, Marian; Kromka, Alexander; Hadraba, Daniel; Svindrych, Zdenek; Burdikova, Zuzana; Bacakova, Lucie

2015-01-01

Nanocrystalline diamond (NCD) films are promising materials for bone implant coatings because of their biocompatibility, chemical resistance, and mechanical hardness. Moreover, NCD wettability can be tailored by grafting specific atoms. The NCD films used in this study were grown on silicon substrates by microwave plasma-enhanced chemical vapor deposition and grafted by hydrogen atoms (H-termination) or oxygen atoms (O-termination). Human osteoblast-like Saos-2 cells were used for biological studies on H-terminated and O-terminated NCD films. The adhesion, growth, and subsequent differentiation of the osteoblasts on NCD films were examined, and the extracellular matrix production and composition were quantified. The osteoblasts that had been cultivated on the O-terminated NCD films exhibited a higher growth rate than those grown on the H-terminated NCD films. The mature collagen fibers were detected in Saos-2 cells on both the H-terminated and O-terminated NCD films; however, the quantity of total collagen in the extracellular matrix was higher on the O-terminated NCD films, as were the amounts of calcium deposition and alkaline phosphatase activity. Nevertheless, the expression of genes for osteogenic markers – type I collagen, alkaline phosphatase, and osteocalcin – was either comparable on the H-terminated and O-terminated films or even lower on the O-terminated films. In conclusion, the higher wettability of the O-terminated NCD films is promising for adhesion and growth of osteoblasts. In addition, the O-terminated surface also seems to support the deposition of extracellular matrix proteins and extracellular matrix mineralization, and this is promising for better osteoconductivity of potential bone implant coatings. PMID:25670900

Does Individual Gambling Behavior Vary across Gambling Venues with Differing Numbers of Terminals? An Empirical Real-World Study using Player Account Data.

PubMed

Sagoe, Dominic; Pallesen, Ståle; Griffiths, Mark D; Mentzoni, Rune A; Leino, Tony

2018-01-01

Research examining gambling behavior via experiments, self-report, and/or observation presents many methodical challenges particularly in relation to objectivity. However, the use of player account-based gambling data provides purely objective data. Based on this real-world data, the primary aim of the present study was to examine gambling behavior in gambling venues with different numbers of gambling terminals (i.e., venues with one terminal; 2-5 terminals; 6-10 terminals; 11-16 terminals). Player account-based gambling data aggregated over a year (2015) amounting to 153,379 observations within 93,034 individual gamblers (males = 74%; mean age = 44.1, SD = 16.4 years) were analyzed. Gambling frequency was highest in venues with 2-5 terminals (54.5%) and lowest in venues with 11-16 terminals (1.6%). Approximately half of the sample (52.5%) gambled in only one venue category, with the majority (81.5%) preferring venues with 2-5 terminals present. Only 0.8% of the sample gambled in all four venue categories. Compared to venues with one terminal, venues with two or more terminals were associated with gamblers placing more bets, and spending more time and money per session. However, gamblers had higher losses (albeit small) in venues with one terminal compared to venues with 2-5 terminals. No differences in net outcome were found between venues with one terminal and those with 6-10 and 11-16 terminals. Overall, the present study demonstrates that in the natural gambling environment, gambling behavior is reinforced in venues with multiple terminals.

Problem-Solving Test: The Mechanism of Transcription Termination by the Rho Factor

ERIC Educational Resources Information Center

Szeberenyi, Jozsef

2012-01-01

Transcription termination comes in two forms in "E. coli" cells. Rho-dependent termination requires the binding of a termination protein called Rho factor to the transcriptional machinery at the terminator region, whereas Rho-independent termination is achieved by conformational changes in the transcript itself. This article presents a test…

Unusually long-lived pause required for regulation of a Rho-dependent transcription terminator.

PubMed

Hollands, Kerry; Sevostiyanova, Anastasia; Groisman, Eduardo A

2014-05-13

Up to half of all transcription termination events in bacteria rely on the RNA-dependent helicase Rho. However, the nucleic acid sequences that promote Rho-dependent termination remain poorly characterized. Defining the molecular determinants that confer Rho-dependent termination is especially important for understanding how such terminators can be regulated in response to specific signals. Here, we identify an extraordinarily long-lived pause at the site where Rho terminates transcription in the 5'-leader region of the Mg(2+) transporter gene mgtA in Salmonella enterica. We dissect the sequence elements required for prolonged pausing in the mgtA leader and establish that the remarkable longevity of this pause is required for a riboswitch to stimulate Rho-dependent termination in the mgtA leader region in response to Mg(2+) availability. Unlike Rho-dependent terminators described previously, where termination occurs at multiple pause sites, there is a single site of transcription termination directed by Rho in the mgtA leader. Our data suggest that Rho-dependent termination events that are subject to regulation may require elements distinct from those operating at constitutive Rho-dependent terminators.

Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator.

PubMed

Li, Rui; Zhang, Qing; Li, Junbai; Shi, Hualin

2016-04-07

An experimental system was designed to measure in vivo termination efficiency (TE) of the Rho-independent terminator and position-function relations were quantified for the terminator tR2 in Escherichia coli The terminator function was almost completely repressed when tR2 was located several base pairs downstream from the gene, and TE gradually increased to maximum values with the increasing distance between the gene and terminator. This TE-distance relation reflected a stochastic coupling of the ribosome and RNA polymerase (RNAP). Terminators located in the first 100 bp of the coding region can function efficiently. However, functional repression was observed when the terminator was located in the latter part of the coding region, and the degree of repression was determined by transcriptional and translational dynamics. These results may help to elucidate mechanisms of Rho-independent termination and reveal genomic locations of terminators and functions of the sequence that precedes terminators. These observations may have important applications in synthetic biology. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

Does Individual Gambling Behavior Vary across Gambling Venues with Differing Numbers of Terminals? An Empirical Real-World Study using Player Account Data

PubMed Central

Sagoe, Dominic; Pallesen, Ståle; Griffiths, Mark D.; Mentzoni, Rune A.; Leino, Tony

2018-01-01

Research examining gambling behavior via experiments, self-report, and/or observation presents many methodical challenges particularly in relation to objectivity. However, the use of player account-based gambling data provides purely objective data. Based on this real-world data, the primary aim of the present study was to examine gambling behavior in gambling venues with different numbers of gambling terminals (i.e., venues with one terminal; 2–5 terminals; 6–10 terminals; 11–16 terminals). Player account-based gambling data aggregated over a year (2015) amounting to 153,379 observations within 93,034 individual gamblers (males = 74%; mean age = 44.1, SD = 16.4 years) were analyzed. Gambling frequency was highest in venues with 2–5 terminals (54.5%) and lowest in venues with 11–16 terminals (1.6%). Approximately half of the sample (52.5%) gambled in only one venue category, with the majority (81.5%) preferring venues with 2–5 terminals present. Only 0.8% of the sample gambled in all four venue categories. Compared to venues with one terminal, venues with two or more terminals were associated with gamblers placing more bets, and spending more time and money per session. However, gamblers had higher losses (albeit small) in venues with one terminal compared to venues with 2–5 terminals. No differences in net outcome were found between venues with one terminal and those with 6–10 and 11–16 terminals. Overall, the present study demonstrates that in the natural gambling environment, gambling behavior is reinforced in venues with multiple terminals. PMID:29503626

Definition of RNA Polymerase II CoTC Terminator Elements in the Human Genome

PubMed Central

Nojima, Takayuki; Dienstbier, Martin; Murphy, Shona; Proudfoot, Nicholas J.; Dye, Michael J.

2013-01-01

Summary Mammalian RNA polymerase II (Pol II) transcription termination is an essential step in protein-coding gene expression that is mediated by pre-mRNA processing activities and DNA-encoded terminator elements. Although much is known about the role of pre-mRNA processing in termination, our understanding of the characteristics and generality of terminator elements is limited. Whereas promoter databases list up to 40,000 known and potential Pol II promoter sequences, fewer than ten Pol II terminator sequences have been described. Using our knowledge of the human β-globin terminator mechanism, we have developed a selection strategy for mapping mammalian Pol II terminator elements. We report the identification of 78 cotranscriptional cleavage (CoTC)-type terminator elements at endogenous gene loci. The results of this analysis pave the way for the full understanding of Pol II termination pathways and their roles in gene expression. PMID:23562152

Evolvable circuit with transistor-level reconfigurability

NASA Technical Reports Server (NTRS)

Stoica, Adrian (Inventor); Salazar-Lazaro, Carlos Harold (Inventor)

2004-01-01

An evolvable circuit includes a plurality of reconfigurable switches, a plurality of transistors within a region of the circuit, the plurality of transistors having terminals, the plurality of transistors being coupled between a power source terminal and a power sink terminal so as to be capable of admitting power between the power source terminal and the power sink terminal, the plurality of transistors being coupled so that every transistor terminal to transistor terminal coupling within the region of the circuit comprises a reconfigurable switch.

AT-rich sequence elements promote nascent transcript cleavage leading to RNA polymerase II termination

PubMed Central

White, Eleanor; Kamieniarz-Gdula, Kinga; Dye, Michael J.; Proudfoot, Nick J.

2013-01-01

RNA Polymerase II (Pol II) termination is dependent on RNA processing signals as well as specific terminator elements located downstream of the poly(A) site. One of the two major terminator classes described so far is the Co-Transcriptional Cleavage (CoTC) element. We show that homopolymer A/T tracts within the human β-globin CoTC-mediated terminator element play a critical role in Pol II termination. These short A/T tracts, dispersed within seemingly random sequences, are strong terminator elements, and bioinformatics analysis confirms the presence of such sequences in 70% of the putative terminator regions (PTRs) genome-wide. PMID:23258704

Dynamic probability of reinforcement for cooperation: Random game termination in the centipede game.

PubMed

Krockow, Eva M; Colman, Andrew M; Pulford, Briony D

2018-03-01

Experimental games have previously been used to study principles of human interaction. Many such games are characterized by iterated or repeated designs that model dynamic relationships, including reciprocal cooperation. To enable the study of infinite game repetitions and to avoid endgame effects of lower cooperation toward the final game round, investigators have introduced random termination rules. This study extends previous research that has focused narrowly on repeated Prisoner's Dilemma games by conducting a controlled experiment of two-player, random termination Centipede games involving probabilistic reinforcement and characterized by the longest decision sequences reported in the empirical literature to date (24 decision nodes). Specifically, we assessed mean exit points and cooperation rates, and compared the effects of four different termination rules: no random game termination, random game termination with constant termination probability, random game termination with increasing termination probability, and random game termination with decreasing termination probability. We found that although mean exit points were lower for games with shorter expected game lengths, the subjects' cooperativeness was significantly reduced only in the most extreme condition with decreasing computer termination probability and an expected game length of two decision nodes. © 2018 Society for the Experimental Analysis of Behavior.

Unusually long-lived pause required for regulation of a Rho-dependent transcription terminator

PubMed Central

Hollands, Kerry; Sevostiyanova, Anastasia; Groisman, Eduardo A.

2014-01-01

Up to half of all transcription termination events in bacteria rely on the RNA-dependent helicase Rho. However, the nucleic acid sequences that promote Rho-dependent termination remain poorly characterized. Defining the molecular determinants that confer Rho-dependent termination is especially important for understanding how such terminators can be regulated in response to specific signals. Here, we identify an extraordinarily long-lived pause at the site where Rho terminates transcription in the 5′-leader region of the Mg2+ transporter gene mgtA in Salmonella enterica. We dissect the sequence elements required for prolonged pausing in the mgtA leader and establish that the remarkable longevity of this pause is required for a riboswitch to stimulate Rho-dependent termination in the mgtA leader region in response to Mg2+ availability. Unlike Rho-dependent terminators described previously, where termination occurs at multiple pause sites, there is a single site of transcription termination directed by Rho in the mgtA leader. Our data suggest that Rho-dependent termination events that are subject to regulation may require elements distinct from those operating at constitutive Rho-dependent terminators. PMID:24778260

Confocal Laser Scanning Microscopy and Ultrastructural Study of VGLUT2 Thalamic Input to Striatal Projection Neurons in Rats

PubMed Central

Lei, Wanlong; Deng, Yunping; Liu, Bingbing; Mu, Shuhua; Guley, Natalie M.; Wong, Ting; Reiner, Anton

2014-01-01

We examined thalamic input to striatum in rats using immunolabeling for the vesicular glutamate transporter (VGLUT2). Double immunofluorescence viewed with confocal laser scanning microscopy (CLSM) revealed that VGLUT2+ terminals are distinct from VGLUT1+ terminals. CLSM of Phaseolus vulgaris-leucoagglutinin (PHAL)-labeled cortical or thalamic terminals revealed that VGLUT2 is rare in corticostriatal terminals but nearly always present in thalamostriatal terminals. Electron microscopy revealed that VGLUT2+ terminals made up 39.4% of excitatory terminals in striatum (with VGLUT1+ corticostriatal terminals constituting the rest), and 66.8% of VGLUT2+ terminals synapsed on spines and the remainder on dendrites. VGLUT2+ axo-spinous terminals had a mean diameter of 0.624 lm, while VGLUT2+ axodendritic terminals a mean diameter of 0.698 µm. In tissue in which we simultaneously immunolabeled thalamostriatal terminals for VGLUT2 and striatal neurons for D1 (with about half of spines immunolabeled for D1), 54.6% of VGLUT2+ terminals targeted D1+ spines (i.e., direct pathway striatal neurons), and 37.3% of D1+ spines received VGLUT2+ synaptic contacts. By contrast, 45.4% of VGLUT2+ terminals targeted D1-negative spines (i.e., indirect pathway striatal neurons), and only 25.8% of D1-negative spines received VGLUT2+ synaptic contacts. Similarly, among VGLUT2+ axodendritic synaptic terminals, 59.1% contacted D1+ dendrites, and 40.9% contacted D1-negative dendrites. VGLUT2+ terminals on D1+ spines and dendrites tended to be slightly smaller than those on D1-negative spines and dendrites. Thus, thala-mostriatal terminals contact both direct and indirect pathway striatal neurons, with a slight preference for direct. These results are consistent with physiological studies indicating slightly different effects of thalamic input on the two types of striatal projection neurons. PMID:23047588

Confocal laser scanning microscopy and ultrastructural study of VGLUT2 thalamic input to striatal projection neurons in rats.

PubMed

Lei, Wanlong; Deng, Yunping; Liu, Bingbing; Mu, Shuhua; Guley, Natalie M; Wong, Ting; Reiner, Anton

2013-04-15

We examined thalamic input to striatum in rats using immunolabeling for the vesicular glutamate transporter (VGLUT2). Double immunofluorescence viewed with confocal laser scanning microscopy (CLSM) revealed that VGLUT2+ terminals are distinct from VGLUT1+ terminals. CLSM of Phaseolus vulgaris-leucoagglutinin (PHAL)-labeled cortical or thalamic terminals revealed that VGLUT2 is rare in corticostriatal terminals but nearly always present in thalamostriatal terminals. Electron microscopy revealed that VGLUT2+ terminals made up 39.4% of excitatory terminals in striatum (with VGLUT1+ corticostriatal terminals constituting the rest), and 66.8% of VGLUT2+ terminals synapsed on spines and the remainder on dendrites. VGLUT2+ axospinous terminals had a mean diameter of 0.624 μm, while VGLUT2+ axodendritic terminals a mean diameter of 0.698 μm. In tissue in which we simultaneously immunolabeled thalamostriatal terminals for VGLUT2 and striatal neurons for D1 (with about half of spines immunolabeled for D1), 54.6% of VGLUT2+ terminals targeted D1+ spines (i.e., direct pathway striatal neurons), and 37.3% of D1+ spines received VGLUT2+ synaptic contacts. By contrast, 45.4% of VGLUT2+ terminals targeted D1-negative spines (i.e., indirect pathway striatal neurons), and only 25.8% of D1-negative spines received VGLUT2+ synaptic contacts. Similarly, among VGLUT2+ axodendritic synaptic terminals, 59.1% contacted D1+ dendrites, and 40.9% contacted D1-negative dendrites. VGLUT2+ terminals on D1+ spines and dendrites tended to be slightly smaller than those on D1-negative spines and dendrites. Thus, thalamostriatal terminals contact both direct and indirect pathway striatal neurons, with a slight preference for direct. These results are consistent with physiological studies indicating slightly different effects of thalamic input on the two types of striatal projection neurons. Copyright © 2012 Wiley Periodicals, Inc.

The dimerization of half-molecule fragments of transferrin.

PubMed Central

Williams, J; Moreton, K

1988-01-01

Partial proteolysis was used to prepare half-molecule fragments of hen ovotransferrin. N-Terminal and C-terminal fragments associate to form an N-terminal fragment-C-terminal fragment dimer. Variant forms of the N- and C-terminal fragments can be prepared in which a few amino acid residues are lacking from the C-terminal ends of the fragments. These variant fragments are partially or completely unable to associate; the suggestion that the molecular recognition sites are located in these C-terminal stretches of the N-terminal half-molecule (320-332) and of the C-terminal half-molecule (683-686) is in agreement with X-ray-crystallography data for human lactotransferrin [Anderson, Baker, Dodson, Norris, Rumball, Waters & Baker (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 1769-1773]. PMID:3415649

Low inductance busbar assembly

DOEpatents

Holbrook, Meghan Ann

2010-09-21

A busbar assembly for electrically coupling first and second busbars to first and second contacts, respectively, on a power module is provided. The assembly comprises a first terminal integrally formed with the first busbar, a second terminal integrally formed with the second busbar and overlapping the first terminal, a first bridge electrode having a first tab electrically coupled to the first terminal and overlapping the first and second terminals, and a second tab electrically coupled to the first contact, a second bridge electrode having a third tab electrically coupled to the second terminal, and overlapping the first and second terminals and the first tab, and a fourth tab electrically coupled to the second contact, and a fastener configured to couple the first tab to the first terminal, and the third tab to the second terminal.

The NH2-terminal and COOH-terminal fragments of dentin matrix protein 1 (DMP1) localize differently in the compartments of dentin and growth plate of bone.

PubMed

Maciejewska, Izabela; Cowan, Cameron; Svoboda, Kathy; Butler, William T; D'Souza, Rena; Qin, Chunlin

2009-02-01

Multiple studies have shown that dentin matrix protein 1 (DMP1) is essential for bone and dentin mineralization. After post-translational proteolytic cleavage, DMP1 exists within the extracellular matrix of bone and dentin as an NH2-terminal fragment, a COOH-terminal fragment, and the proteoglycan form of the NH2-terminal fragment (DMP1-PG). To begin to assess the biological function of each fragment, we evaluated the distribution of both fragments in the rat tooth and bone using antibodies specific to the NH2-terminal and COOH-terminal regions of DMP1 and confocal microscopy. In rat first molar organs, the NH2-terminal fragment localized to predentin, whereas the COOH-terminal fragment was mainly restricted to mineralized dentin. In the growth plate of bone, the NH2-terminal fragment appeared in the proliferation and hypertrophic zones, whereas the COOH-terminal fragment occupied the ossification zone. Forster resonance energy transfer analysis showed colocalization of both fragments of DMP1 in odontoblasts and predentin, as well as hypertrophic chondrocytes within the growth plates of bone. The biochemical analysis of bovine teeth showed that predentin is rich in DMP1-PG, whereas mineralized dentin primarily contains the COOH-terminal fragment. We conclude that the differential patterns of expression of NH2-terminal and COOH-terminal fragments of DMP1 reflect their potentially distinct roles in the biomineralization of dentin and bone matrices.

Effect of Ge surface termination on oxidation behavior

NASA Astrophysics Data System (ADS)

Lee, Younghwan; Park, Kibyung; Cho, Yong Soo; Lim, Sangwoo

2008-09-01

Sulfur-termination was formed on the Ge(1 0 0) surface using (NH 4) 2S solution. Formation of Ge-S and the oxidation of the S-terminated Ge surface were monitored with multiple internal reflection Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. In the 0.5, 5, or 20% (NH 4) 2S solution, H-termination on the Ge(1 0 0) surface was substituted with S-termination in 1 min. When the S-terminated Ge(1 0 0) surface was exposed in air ambient, the oxidation was retarded for about 3600 min. The preservation time of the oxide layer up to one monolayer of S-terminated Ge(1 0 0) surface was about 120 times longer than for the H-terminated Ge(1 0 0) surface. However, the oxidation of S-terminated Ge(1 0 0) surface drastically increased after the threshold time. There was no significant difference in threshold time between S-terminations formed in 0.5, 5, and 20% (NH 4) 2S solutions. With the surface oxidation, desorption of S on the Ge surface was observed. The desorption behavior of sulfur on the S-terminated Ge(1 0 0) surface was independent of the concentration of the (NH 4) 2S solution that forms S-termination. Non-ideal S-termination on Ge surfaces may be related to drastic oxidation of the Ge surface. Finally, with the desulfurization on the S-terminated Ge(1 0 0) surface, oxide growth is accelerated.

Psychiatrists and termination of pregnancy: clinical, legal and ethical aspects.

PubMed

Morris, Kirsty; Savell, Kristin; Ryan, Christopher J

2012-01-01

To provide practical guidance for psychiatrists asked to conduct an assessment of a woman requesting a termination of pregnancy. The law relevant to termination of pregnancy in each of the Australian states and territories and in New Zealand was synthesised and reviewed, as was the available literature around the key roles for the psychiatrist in these settings. Little is known about the rates of and reasons for terminations in Australasia. The 'lawfulness' of termination varies between jurisdictions and might require a consideration of the woman's mental health. When psychiatrists are asked to assist, their roles can be divided into: assessment and management of the woman's mental health, assessment of the woman's capacity to consent to the termination, assessment of the impact of having a termination or not having a termination upon the woman's mental health, assistance in determining the lawfulness of the proposed termination and, finally, support of the obstetric team providing terminations. The psychiatric assessment of a woman requesting a termination of pregnancy requires an understanding of the ethical issues, the relevant law and a clinical framework within which the psychiatrist can delineate his or her various roles.

Injury Outcome in Crashes with Guardrail End Terminals.

PubMed

Johnson, Nicholas S; Gabler, Hampton C

2015-01-01

The goal of this study is to evaluate the crash performance of guardrail end terminals in real-world crashes. Guardrail end terminals are installed at the ends of guardrail systems to prevent the rail from spearing through the car in an end-on collision. Recently, there has been a great deal of controversy as to the safety of certain widely used end terminal designs, partly because there is surprisingly little real-world crash data for end terminals. Most existing studies of end terminal crashes used data from prior to the mid-1990s. Since then, there have been large improvements to vehicle crashworthiness and seat belt usage rates, as well as new roadside safety hardware compliant with National Cooperative Highway Research Program (NCHRP) Report 350, "Recommended Procedures for the Safety Performance Evaluation of Highway Features." Additionally, most existing studies of injury in end terminal crashes do not account for factors such as the occurrence of rollover. This analysis uses more recent crash data that represent post-1990s vehicle fleet changes and account for a number of factors that may affect driver injury outcome and rollover occurrence. Passenger vehicle crashes coded as involving guardrail end terminals were identified in the set of police-reported crashes in Michigan in 2011 and 2012. End terminal performance was expected to be a function of end terminal system design. State crash databases generally do not identify specific end terminal systems. In this study, the coded crash location was used to obtain photographs of the crash site prior to the crash from Google Street View. These site photographs were manually inspected to identify the particular end terminal system involved in the crash. Multiple logistic regression was used to test for significant differences in the odds of driver injury and rollover between different terminal types while accounting for other factors. A total of 1,001 end terminal crashes from the 2011-2012 Michigan State crash data were manually inspected to identify the terminal that had been struck. Four hundred fifty-one crashes were found to be suitable for analysis. Serious to fatal driver injury occurred in 3.8% of end terminal crashes, moderate to fatal driver injury occurred in 11.8%, and 72.3% involved property damage only. No significant difference in moderate to fatal driver injury odds was observed between NCHRP 350 compliant end terminals and noncompliant terminals. Car drivers showed odds of moderate to fatal injury 3.6 times greater than LTV drivers in end terminal crashes. Rollover occurrence was not significantly associated with end terminal type. Car drivers have greater potential for injury in end terminal crashes than light truck/van/sport utility vehicle drivers. End terminal designs compliant with NCHRP 350 did not appear to carry different odds of moderate driver injury than noncompliant end terminals. The findings account for driver seat belt use, rollover occurrence, terminal orientation (leading/trailing), control loss, and the number of impact events. Rollover and nonuse of seat belts carried much larger increases in injury potential than end terminal type. Rollover did not appear to be associated with NCHRP 350 compliance.

How do late terminations of pregnancy affect comparisons of stillbirth rates in Europe? Analyses of aggregated routine data from the Euro-Peristat Project.

PubMed

Blondel, B; Cuttini, M; Hindori-Mohangoo, A D; Gissler, M; Loghi, M; Prunet, C; Heino, A; Smith, L; van der Pal-de Bruin, K; Macfarlane, A; Zeitlin, J

2018-01-01

To describe how terminations of pregnancy at gestational ages at or above the limit for stillbirth registration are recorded in routine statistics and to assess their impact on comparability of stillbirth rates in Europe. Analysis of aggregated data from the Euro-Peristat project. Twenty-nine European countries. Births and late terminations in 2010. Assessment of terminations as a proportion of stillbirths and derivation of stillbirth rates including and excluding terminations. Stillbirth rates overall and excluding terminations. In 23 countries, it is possible to assess the contribution of terminations to stillbirth rates either because terminations are rare occurrences or because they can be distinguished from spontaneous stillbirths. Where terminations were reported, they accounted for less than 1.5% of stillbirths at 22+ weeks in Denmark, between 13 and 22% in Germany, Italy, Hungary, Finland and Switzerland, and 39% in France. Proportions were much lower at 24+ weeks, with the exception of Switzerland (7.4%) and France (39.2%). Terminations represent a substantial proportion of stillbirths at 22+ weeks of gestation in some countries. Countries where terminations occur at 22+ weeks should publish rates with and without terminations in order to improve international comparisons and the policy relevance of stillbirth statistics. For valid comparisons of stillbirth rates, data about late terminations of pregnancy are needed. © 2017 Royal College of Obstetricians and Gynaecologists.

7 CFR 947.71 - Termination.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Effective Time and Termination § 947.71 Termination. (a) The Secretary may at any time terminate the... of such potatoes produced for market; but such termination shall be effective only if announced on or...

Graphics Software For VT Terminals

NASA Technical Reports Server (NTRS)

Wang, Caroline

1991-01-01

VTGRAPH graphics software tool for DEC/VT computer terminal or terminals compatible with it, widely used by government and industry. Callable in FORTRAN or C language, library program enabling user to cope with many computer environments in which VT terminals used for window management and graphic systems. Provides PLOT10-like package plus color or shade capability for VT240, VT241, and VT300 terminals. User can easily design more-friendly user-interface programs and design PLOT10 programs on VT terminals with different computer systems. Requires ReGis graphics set terminal and FORTRAN compiler.

Diode-quad bridge circuit means

NASA Technical Reports Server (NTRS)

Harrison, D. R.; Dimeff, J. (Inventor)

1975-01-01

Diode-quad bridge circuit means is described for use as a transducer circuit or as a discriminator circuit. It includes: (1) a diode bridge having first, second, third, and fourth bridge terminals consecutively coupled together by four diodes polarized in circulating relationship; (2) a first impedance connected between the second bridge terminal and a circuit ground; (3) a second impedance connected between the fourth bridge terminal and the circuit ground; (4) a signal source having a first source terminal capacitively coupled to the first and third bridge terminals, and a second source terminal connected to the circuit ground; and (5) an output terminal coupled to the first bridge terminal and at which an output signal may be taken.

Fail-safe transcription termination: Because one is never enough.

PubMed

Lemay, Jean-François; Bachand, François

2015-01-01

Termination of RNA polymerase II (RNAPII) transcription is a fundamental step of gene expression that involves the release of the nascent transcript and dissociation of RNAPII from the DNA template. As transcription termination is intimately linked to RNA 3' end processing, termination pathways have a key decisive influence on the fate of the transcribed RNA. Quite remarkably, when reaching the 3' end of genes, a substantial fraction of RNAPII fail to terminate transcription, requiring the contribution of alternative or "fail-safe" mechanisms of termination to release the polymerase. This point of view covers redundant mechanisms of transcription termination and how they relate to conventional termination models. In particular, we expand on recent findings that propose a reverse torpedo model of termination, in which the 3'5' exonucleolytic activity of the RNA exosome targets transcription events associated with paused and backtracked RNAPII.

Determination of the termination efficiency of the transcription terminator using different fluorescent profiles in green fluorescent protein mutants.

PubMed

Nojima, Takahiko; Lin, Angela C; Fujii, Teruo; Endo, Isao

2005-12-01

An approach in determining the intrinsic termination efficiency (%T) of transcription termination using green fluorescent protein (GFP) mutants was developed. This approach utilizes a cassette vector in which the tested terminator is introduced between two GFP mutant genes: an ultraviolet-optimized mutant (GFPuv: F99S, M153T, V163A) and a blue-shifted mutant (BFP: F64L, S65T, T145F). The ratio of the fluorescence intensity of BFP to GFPuv after transcription and translation represents the termination efficiency of the terminator. E. coli ribosomal RNA operon T1 terminator, phage lambda terminator site R2, E. coli tryptophane attenuater were introduced into the vector, and their transcriptional efficiencies were estimated as 89, 79, and 24%, respectively, showing good agreement with published data.

Terminating Devices in Spoken French.

ERIC Educational Resources Information Center

Andrews, Barry J.

1989-01-01

A study examines the way in which one group of discourse connectors, terminators, function in contemporary spoken French. Three types of terminators, elements used at the end of an utterance or section to indicate its completion, are investigated, including utterance terminators, interrogative tags, and terminal tags. (Author/MSE)

20 CFR 435.61 - Termination.

Code of Federal Regulations, 2010 CFR

2010-04-01

... INSTITUTIONS OF HIGHER EDUCATION, HOSPITALS, OTHER NON-PROFIT ORGANIZATIONS, AND COMMERCIAL ORGANIZATIONS Post... the effective date and, in the case of partial termination, the portion to be terminated. (3) By the... effective date, and, in the case of partial termination, the portion to be terminated. However, if SSA...

45 CFR 1210.3-1 - Grounds for termination.

Code of Federal Regulations, 2012 CFR

2012-10-01

... SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-1 Grounds for termination. ACTION may terminate or suspend a Volunteer based on the Volunteer's conduct for the following reasons: (a) Conviction of any criminal offense under Federal, State...

45 CFR 1210.3-1 - Grounds for termination.

Code of Federal Regulations, 2010 CFR

2010-10-01

... SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-1 Grounds for termination. ACTION may terminate or suspend a Volunteer based on the Volunteer's conduct for the following reasons: (a) Conviction of any criminal offense under Federal, State...

45 CFR 1210.3-1 - Grounds for termination.

Code of Federal Regulations, 2011 CFR

2011-10-01

... SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-1 Grounds for termination. ACTION may terminate or suspend a Volunteer based on the Volunteer's conduct for the following reasons: (a) Conviction of any criminal offense under Federal, State...

45 CFR 1210.3-1 - Grounds for termination.

Code of Federal Regulations, 2014 CFR

2014-10-01

... SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-1 Grounds for termination. ACTION may terminate or suspend a Volunteer based on the Volunteer's conduct for the following reasons: (a) Conviction of any criminal offense under Federal, State...

45 CFR 1210.3-1 - Grounds for termination.

Code of Federal Regulations, 2013 CFR

2013-10-01

... SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-1 Grounds for termination. ACTION may terminate or suspend a Volunteer based on the Volunteer's conduct for the following reasons: (a) Conviction of any criminal offense under Federal, State...

48 CFR 49.601-2 - Letter notice.

Code of Federal Regulations, 2010 CFR

2010-10-01

... notice of termination is suggested for use if a contract for supplies is being terminated for convenience... termination; e.g., name and address of company, contract number of terminated contract, items, etc.] (a...'s convenience under the clause entitled _________ [insert title of appropriate termination clause...

7 CFR 947.72 - Proceedings after termination.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Proceedings after termination. 947.72 Section 947.72 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... Effective Time and Termination § 947.72 Proceedings after termination. (a) Upon the termination of the...

Yeast Terminator Function Can Be Modulated and Designed on the Basis of Predictions of Nucleosome Occupancy.

PubMed

Morse, Nicholas J; Gopal, Madan R; Wagner, James M; Alper, Hal S

2017-11-17

The design of improved synthetic parts is a major goal of synthetic biology. Mechanistically, nucleosome occupancy in the 3' terminator region of a gene has been found to correlate with transcriptional expression. Here, we seek to establish a predictive relationship between terminator function and predicted nucleosome positioning to design synthetic terminators in the yeast Saccharomyces cerevisiae. In doing so, terminators improved net protein output from these expression cassettes nearly 4-fold over their original sequence with observed increases in termination efficiency to 96%. The resulting terminators were indeed depleted of nucleosomes on the basis of mapping experiments. This approach was successfully applied to synthetic, de novo, and native terminators. The mode of action of these modifications was mainly through increased termination efficiency, rather than half-life increases, perhaps suggesting a role in improved mRNA maturation. Collectively, these results suggest that predicted nucleosome depletion can be used as a heuristic approach for improving terminator function, though the underlying mechanism remains to be shown.

Definition of RNA polymerase II CoTC terminator elements in the human genome.

PubMed

Nojima, Takayuki; Dienstbier, Martin; Murphy, Shona; Proudfoot, Nicholas J; Dye, Michael J

2013-04-25

Mammalian RNA polymerase II (Pol II) transcription termination is an essential step in protein-coding gene expression that is mediated by pre-mRNA processing activities and DNA-encoded terminator elements. Although much is known about the role of pre-mRNA processing in termination, our understanding of the characteristics and generality of terminator elements is limited. Whereas promoter databases list up to 40,000 known and potential Pol II promoter sequences, fewer than ten Pol II terminator sequences have been described. Using our knowledge of the human β-globin terminator mechanism, we have developed a selection strategy for mapping mammalian Pol II terminator elements. We report the identification of 78 cotranscriptional cleavage (CoTC)-type terminator elements at endogenous gene loci. The results of this analysis pave the way for the full understanding of Pol II termination pathways and their roles in gene expression. Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.

Bayesian Approach for Flexible Modeling of Semicompeting Risks Data

PubMed Central

Han, Baoguang; Yu, Menggang; Dignam, James J.; Rathouz, Paul J.

2016-01-01

Summary Semicompeting risks data arise when two types of events, non-terminal and terminal, are observed. When the terminal event occurs first, it censors the non-terminal event, but not vice versa. To account for possible dependent censoring of the non-terminal event by the terminal event and to improve prediction of the terminal event using the non-terminal event information, it is crucial to model their association properly. Motivated by a breast cancer clinical trial data analysis, we extend the well-known illness-death models to allow flexible random effects to capture heterogeneous association structures in the data. Our extension also represents a generalization of the popular shared frailty models that usually assume that the non-terminal event does not affect the hazards of the terminal event beyond a frailty term. We propose a unified Bayesian modeling approach that can utilize existing software packages for both model fitting and individual specific event prediction. The approach is demonstrated via both simulation studies and a breast cancer data set analysis. PMID:25274445

Improvement in wettability of porous Si by carboxylate termination

NASA Astrophysics Data System (ADS)

Sakakibara, Masanori; Matsumoto, Kimihisa; Kamiya, Kazuhide; Kawabata, Shigeki; Inada, Mitsuru; Suzuki, Shinya

2018-02-01

The effects of the surface terminations of carboxylic acid and carboxylate on the hydrophilicity of porous Si were studied to observe the changes in the photoluminescence (PL) intensity of water-dispersed porous Si powder over time. Porous Si terminated by carboxylate was produced from carboxylic acid-terminated porous Si by a neutralization reaction with an alkali metal. After the neutralization of porous Si terminated by carboxylic acid, the formation of carboxylate-terminated porous Si was confirmed by observing the absorption peaks corresponding to Si-C and COO- from Fourier transform infrared (FT-IR) spectra. On the basis of changes in the PL intensity of porous Si over time, the hydrophilicity of porous Si terminated by carboxylate was determined to be higher than that of porous Si terminated by carboxylic acid. On the other hand, nonradiative recombination centers on the surface of carboxylate-terminated porous Si were formed during the neutralization process, which reduced the PL intensity. The PL from porous Si terminated by carboxylic acid and carboxylate was caused by the quantum size effect regardless of the termination molecules, which was confirmed by the wavelength dependence of the PL lifetime. Porous Si terminated by undecylenate is an effective material for applications such as bio-labels owing to its hydrophilicity and high PL stability.

A genome-wide activity assessment of terminator regions in Saccharomyces cerevisiae provides a ″terminatome″ toolbox.

PubMed

Yamanishi, Mamoru; Ito, Yoichiro; Kintaka, Reiko; Imamura, Chie; Katahira, Satoshi; Ikeuchi, Akinori; Moriya, Hisao; Matsuyama, Takashi

2013-06-21

The terminator regions of eukaryotes encode functional elements in the 3' untranslated region (3'-UTR) that influence the 3'-end processing of mRNA, mRNA stability, and translational efficiency, which can modulate protein production. However, the contribution of these terminator regions to gene expression remains unclear, and therefore their utilization in metabolic engineering or synthetic genetic circuits has been limited. Here, we comprehensively evaluated the activity of 5302 terminator regions from a total of 5880 genes in the budding yeast Saccharomyces cerevisiae by inserting each terminator region downstream of the P TDH3 - green fluorescent protein (GFP) reporter gene and measuring the fluorescent intensity of GFP. Terminator region activities relative to that of the PGK1 standard terminator ranged from 0.036 to 2.52, with a mean of 0.87. We thus could isolate the most and least active terminator regions. The activities of the terminator regions showed a positive correlation with mRNA abundance, indicating that the terminator region is a determinant of mRNA abundance. The least active terminator regions tended to encode longer 3'-UTRs, suggesting the existence of active degradation mechanisms for those mRNAs. The terminator regions of ribosomal protein genes tended to be the most active, suggesting the existence of a common regulator of those genes. The ″terminatome″ (the genome-wide set of terminator regions) thus not only provides valuable information to understand the modulatory roles of terminator regions on gene expression but also serves as a useful toolbox for the development of metabolically and genetically engineered yeast.

47 CFR 68.354 - Numbering and labeling requirements for terminal equipment.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Conformity or that is certified by a Telecommunications Certification Body shall have labels in a place and manner required by the Administrative Council for Terminal Attachments. (b) Terminal equipment labels... Terminal Attachments. (c) If the Administrative Council for Terminal Attachments chooses to continue the...

43 CFR 12.961 - Termination.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Institutions of Higher Education, Hospitals, and Other Non-Profit Organizations Post-Award Requirements § 12..., including the effective date and, in the case of partial termination, the portion to be terminated. (3) By... reasons for such termination, the effective date, and, in the case of partial termination, the portion to...

76 FR 22120 - Credit Watch Termination Initiative; Termination of Origination Approval Agreements

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-20

... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR- 5511-N-01] Credit Watch Termination Initiative; Termination of Origination Approval Agreements AGENCY: Office of the Assistant Secretary for... (FHA) against HUD-approved mortgagees through the FHA Credit Watch Termination Initiative. This notice...

75 FR 67387 - Credit Watch Termination Initiative Termination of Origination Approval Agreements

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-02

... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR-4211-N-05] Credit Watch Termination Initiative Termination of Origination Approval Agreements AGENCY: Office of the Assistant Secretary for... (FHA) against HUD-approved mortgagees through the FHA Credit Watch Termination Initiative. This notice...

77 FR 38818 - Credit Watch Termination Initiative; Termination of Origination Approval Agreements

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-29

... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR-5644-N-01] Credit Watch Termination Initiative; Termination of Origination Approval Agreements AGENCY: Office of the Assistant Secretary for... (FHA) against HUD-approved mortgagees through the FHA Credit Watch Termination Initiative. This notice...

76 FR 38406 - Credit Watch Termination Initiative; Termination of Origination Approval Agreements

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-30

... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR-5511-N-03] Credit Watch Termination Initiative; Termination of Origination Approval Agreements AGENCY: Office of the Assistant Secretary for... (FHA) against HUD-approved mortgagees through the FHA Credit Watch Termination Initiative. This notice...

76 FR 4126 - Credit Watch Termination Initiative Termination of Origination Approval Agreements

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-24

... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR- 5411-N-07] Credit Watch Termination Initiative Termination of Origination Approval Agreements AGENCY: Office of the Assistant Secretary for... (FHA) against HUD-approved mortgagees through the FHA Credit Watch Termination Initiative. This notice...

77 FR 5263 - Credit Watch Termination Initiative Termination of Origination Approval Agreements

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-02

... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR-5511-N-06] Credit Watch Termination Initiative Termination of Origination Approval Agreements AGENCY: Office of the Assistant Secretary for... (FHA) against HUD-approved mortgagees through the FHA Credit Watch Termination Initiative. This notice...

75 FR 61164 - Credit Watch Termination Initiative Termination of Origination Approval Agreements

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-04

... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR-5411-N-03] Credit Watch Termination Initiative Termination of Origination Approval Agreements AGENCY: Office of the Assistant Secretary for... (FHA) against HUD-approved mortgagees through the FHA Credit Watch Termination Initiative. This notice...

45 CFR 1210.3-11 - Disposition of termination and appeal files.

Code of Federal Regulations, 2012 CFR

2012-10-01

... FOR NATIONAL AND COMMUNITY SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-11 Disposition of termination and appeal files. All... successful termination appeal may be made part of, or included in, a Volunteer's official folder. ...

45 CFR 1210.3-11 - Disposition of termination and appeal files.

Code of Federal Regulations, 2013 CFR

2013-10-01

... FOR NATIONAL AND COMMUNITY SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-11 Disposition of termination and appeal files. All... successful termination appeal may be made part of, or included in, a Volunteer's official folder. ...

45 CFR 1210.3-11 - Disposition of termination and appeal files.

Code of Federal Regulations, 2010 CFR

2010-10-01

... FOR NATIONAL AND COMMUNITY SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-11 Disposition of termination and appeal files. All... successful termination appeal may be made part of, or included in, a Volunteer's official folder. ...

45 CFR 1210.3-11 - Disposition of termination and appeal files.

Code of Federal Regulations, 2011 CFR

2011-10-01

... FOR NATIONAL AND COMMUNITY SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-11 Disposition of termination and appeal files. All... successful termination appeal may be made part of, or included in, a Volunteer's official folder. ...

45 CFR 1210.3-11 - Disposition of termination and appeal files.

Code of Federal Regulations, 2014 CFR

2014-10-01

... FOR NATIONAL AND COMMUNITY SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-11 Disposition of termination and appeal files. All... successful termination appeal may be made part of, or included in, a Volunteer's official folder. ...

48 CFR 49.105 - Duties of termination contracting officer after issuance of notice of termination.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT TERMINATION OF CONTRACTS General Principles 49.105 Duties of termination contracting officer after issuance of notice of termination. (a... principles relating to the settlement of any settlement proposal, including obligations of the contractor...

Death Orientation and Communication with the Terminally Ill.

ERIC Educational Resources Information Center

Eggerman, Sinda; Dustin, Dick

1986-01-01

Examined relationship betwen attitude toward death and the terminal patient and communication with terminally ill in 103 medical students and 15 family physicians. Relationships were found between indices (Terminal Illness Questionnaire, Threat Index) and factors used in determining whether to reveal a terminal diagnosis. (Author/NRB)

Modular space station, phase B extension. Information management advanced development. Volume 2: Communications terminal breadboard

NASA Technical Reports Server (NTRS)

Gerber, C. R.

1972-01-01

The design and development of the communications terminal breadboard for the modular space station are discussed. The subjects presented are: (1) history of communications terminal breadboard, (2) requirements analysis, (3) technology goals in terminal design, and (4) communications terminal board integration tests.

8 CFR 236.18 - Termination of Family Unity Program benefits.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 8 Aliens and Nationality 1 2011-01-01 2011-01-01 false Termination of Family Unity Program... REMOVED Family Unity Program § 236.18 Termination of Family Unity Program benefits. (a) Grounds for termination. The Service may terminate benefits under the Family Unity Program whenever the necessity for the...

Differences between Mutual and Client-Intiated Nonmutual Terminations in a University Counseling Center.

ERIC Educational Resources Information Center

Cochran, Sam V.; Stamler, Virginia Lee

1989-01-01

Examined differences in satisfaction with counseling between clients (N=52) who initiated termination of treatment without discussing termination with their counselors and clients (N=146) who mutually planned termination with counselors. Results revealed that nonmutual terminators perceived counseling less positively and reported different reasons…

76 FR 22119 - Credit Watch Termination Initiative; Termination of Direct Endorsement (DE) Approval

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-20

... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR-5511-N-02] Credit Watch Termination Initiative; Termination of Direct Endorsement (DE) Approval AGENCY: Office of the Assistant Secretary for... (FHA) against HUD-approved mortgagees through the FHA Credit Watch Termination Initiative. This notice...

76 FR 53148 - Credit Watch Termination Initiative; Termination of Direct Endorsement (DE) Approval

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-25

... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR-5511-N-05] Credit Watch Termination Initiative; Termination of Direct Endorsement (DE) Approval AGENCY: Office of the Assistant Secretary for... (FHA) against HUD-approved mortgagees through the FHA Credit Watch Termination Initiative. This notice...

77 FR 5262 - Credit Watch Termination Initiative Termination of Direct Endorsement (DE) Approval

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-02

... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR-5511-N-07] Credit Watch Termination Initiative Termination of Direct Endorsement (DE) Approval AGENCY: Office of the Assistant Secretary for... (FHA) against HUD-approved mortgagees through the FHA Credit Watch Termination Initiative. This notice...

77 FR 38817 - Credit Watch Termination Initiative; Termination of Direct Endorsement (DE) Approval

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-29

... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR-5644-N-02] Credit Watch Termination Initiative; Termination of Direct Endorsement (DE) Approval AGENCY: Office of the Assistant Secretary for... (FHA) against HUD-approved mortgagees through the FHA Credit Watch Termination Initiative. This notice...

76 FR 38407 - Credit Watch Termination Initiative; Termination of Direct Endorsement (DE) Approval

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-30

... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR-5511-N-04] Credit Watch Termination Initiative; Termination of Direct Endorsement (DE) Approval AGENCY: Office of the Assistant Secretary for... (FHA) against HUD-approved mortgagees through the FHA Credit Watch Termination Initiative. This notice...

75 FR 61165 - Credit Watch Termination Initiative Termination of Direct Endorsement (DE) Approval

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-04

... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR-5411-N-04] Credit Watch Termination Initiative Termination of Direct Endorsement (DE) Approval AGENCY: Office of the Assistant Secretary for... (FHA) against HUD-approved mortgagees through the FHA Credit Watch Termination Initiative. This notice...

76 FR 4364 - Credit Watch Termination Initiative; Termination of Direct Endorsement (DE) Approval

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-25

... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR-5411-N-08] Credit Watch Termination Initiative; Termination of Direct Endorsement (DE) Approval AGENCY: Office of the Assistant Secretary for... (FHA) against HUD-approved mortgagees through the FHA Credit Watch Termination Initiative. This notice...

75 FR 67388 - Credit Watch Termination Initiative; Termination of Direct Endorsement (DE) Approval

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-02

... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR-5411-N-06] Credit Watch Termination Initiative; Termination of Direct Endorsement (DE) Approval AGENCY: Office of the Assistant Secretary for... (FHA) against HUD-approved mortgagees through the FHA Credit Watch Termination Initiative. This notice...

14 CFR 1214.108 - Termination.

Code of Federal Regulations, 2011 CFR

2011-01-01

... NASA. (1) The termination fee for dedicated flights will be computed as a percentage of the Shuttle... Space Shuttle Flights of Payloads for Non-U.S. Government, Reimbursable Customers § 1214.108 Termination... termination occurs Termination fee, percent of Shuttle standard flight price 18 or more 10 17 or more but less...

14 CFR 1214.108 - Termination.

Code of Federal Regulations, 2013 CFR

2013-01-01

... NASA. (1) The termination fee for dedicated flights will be computed as a percentage of the Shuttle... Space Shuttle Flights of Payloads for Non-U.S. Government, Reimbursable Customers § 1214.108 Termination... termination occurs Termination fee, percent of Shuttle standard flight price 18 or more 10 17 or more but less...

14 CFR 1214.108 - Termination.

Code of Federal Regulations, 2010 CFR

2010-01-01

... NASA. (1) The termination fee for dedicated flights will be computed as a percentage of the Shuttle... Space Shuttle Flights of Payloads for Non-U.S. Government, Reimbursable Customers § 1214.108 Termination... termination occurs Termination fee, percent of Shuttle standard flight price 18 or more 10 17 or more but less...

14 CFR 1214.108 - Termination.

Code of Federal Regulations, 2012 CFR

2012-01-01

... NASA. (1) The termination fee for dedicated flights will be computed as a percentage of the Shuttle... Space Shuttle Flights of Payloads for Non-U.S. Government, Reimbursable Customers § 1214.108 Termination... termination occurs Termination fee, percent of Shuttle standard flight price 18 or more 10 17 or more but less...

A Plasma Display Terminal.

ERIC Educational Resources Information Center

Stifle, Jack

A graphics terminal designed for use as a remote computer input/output terminal is described. Although the terminal is intended for use in teaching applications, it has several features which make it useful in many other computer terminal applications. These features include: a 10-inch square plasma display panel, permanent storage of information…

8 CFR 236.18 - Termination of Family Unity Program benefits.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 8 Aliens and Nationality 1 2010-01-01 2010-01-01 false Termination of Family Unity Program... REMOVED Family Unity Program § 236.18 Termination of Family Unity Program benefits. (a) Grounds for termination. The Service may terminate benefits under the Family Unity Program whenever the necessity for the...

7 CFR 1703.111 - Grant and loan termination.

Code of Federal Regulations, 2012 CFR

2012-01-01

... AGRICULTURE RURAL DEVELOPMENT Distance Learning and Telemedicine Loan and Grant Program-General § 1703.111... agree upon the conditions of the termination, the effective date of the termination, and, in the case of... such termination, the effective date, and, in the case of a partial termination, the portion of the...

7 CFR 1703.111 - Grant and loan termination.

Code of Federal Regulations, 2011 CFR

2011-01-01

... AGRICULTURE RURAL DEVELOPMENT Distance Learning and Telemedicine Loan and Grant Program-General § 1703.111... agree upon the conditions of the termination, the effective date of the termination, and, in the case of... such termination, the effective date, and, in the case of a partial termination, the portion of the...

7 CFR 1703.111 - Grant and loan termination.

Code of Federal Regulations, 2013 CFR

2013-01-01

... AGRICULTURE RURAL DEVELOPMENT Distance Learning and Telemedicine Loan and Grant Program-General § 1703.111... agree upon the conditions of the termination, the effective date of the termination, and, in the case of... such termination, the effective date, and, in the case of a partial termination, the portion of the...

7 CFR 1703.111 - Grant and loan termination.

Code of Federal Regulations, 2014 CFR

2014-01-01

... AGRICULTURE RURAL DEVELOPMENT Distance Learning and Telemedicine Loan and Grant Program-General § 1703.111... agree upon the conditions of the termination, the effective date of the termination, and, in the case of... such termination, the effective date, and, in the case of a partial termination, the portion of the...

47 CFR 68.610 - Database of terminal equipment.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 3 2011-10-01 2011-10-01 false Database of terminal equipment. 68.610 Section... Attachments § 68.610 Database of terminal equipment. (a) The Administrative Council for Terminal Attachments shall operate and maintain a database of all approved terminal equipment. The database shall meet the...

47 CFR 68.610 - Database of terminal equipment.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 3 2010-10-01 2010-10-01 false Database of terminal equipment. 68.610 Section... Attachments § 68.610 Database of terminal equipment. (a) The Administrative Council for Terminal Attachments shall operate and maintain a database of all approved terminal equipment. The database shall meet the...

47 CFR 68.610 - Database of terminal equipment.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 3 2013-10-01 2013-10-01 false Database of terminal equipment. 68.610 Section... Attachments § 68.610 Database of terminal equipment. (a) The Administrative Council for Terminal Attachments shall operate and maintain a database of all approved terminal equipment. The database shall meet the...

47 CFR 68.610 - Database of terminal equipment.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 3 2014-10-01 2014-10-01 false Database of terminal equipment. 68.610 Section... Attachments § 68.610 Database of terminal equipment. (a) The Administrative Council for Terminal Attachments shall operate and maintain a database of all approved terminal equipment. The database shall meet the...

47 CFR 68.610 - Database of terminal equipment.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 3 2012-10-01 2012-10-01 false Database of terminal equipment. 68.610 Section... Attachments § 68.610 Database of terminal equipment. (a) The Administrative Council for Terminal Attachments shall operate and maintain a database of all approved terminal equipment. The database shall meet the...

46 CFR 535.309 - Marine terminal services agreements-exemption.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 9 2011-10-01 2011-10-01 false Marine terminal services agreements-exemption. 535.309... COMMERCE OCEAN COMMON CARRIER AND MARINE TERMINAL OPERATOR AGREEMENTS SUBJECT TO THE SHIPPING ACT OF 1984 Exemptions § 535.309 Marine terminal services agreements—exemption. (a) Marine terminal services agreement...

46 CFR 535.309 - Marine terminal services agreements-exemption.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 9 2013-10-01 2013-10-01 false Marine terminal services agreements-exemption. 535.309... COMMERCE OCEAN COMMON CARRIER AND MARINE TERMINAL OPERATOR AGREEMENTS SUBJECT TO THE SHIPPING ACT OF 1984 Exemptions § 535.309 Marine terminal services agreements—exemption. (a) Marine terminal services agreement...

46 CFR 535.310 - Marine terminal facilities agreement-exemption.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 9 2010-10-01 2010-10-01 false Marine terminal facilities agreement-exemption. 535.310... COMMERCE OCEAN COMMON CARRIER AND MARINE TERMINAL OPERATOR AGREEMENTS SUBJECT TO THE SHIPPING ACT OF 1984 Exemptions § 535.310 Marine terminal facilities agreement—exemption. (a) Marine terminal facilities agreement...

46 CFR 535.310 - Marine terminal facilities agreement-exemption.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 9 2012-10-01 2012-10-01 false Marine terminal facilities agreement-exemption. 535.310... COMMERCE OCEAN COMMON CARRIER AND MARINE TERMINAL OPERATOR AGREEMENTS SUBJECT TO THE SHIPPING ACT OF 1984 Exemptions § 535.310 Marine terminal facilities agreement—exemption. (a) Marine terminal facilities agreement...

46 CFR 535.310 - Marine terminal facilities agreement-exemption.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 9 2011-10-01 2011-10-01 false Marine terminal facilities agreement-exemption. 535.310... COMMERCE OCEAN COMMON CARRIER AND MARINE TERMINAL OPERATOR AGREEMENTS SUBJECT TO THE SHIPPING ACT OF 1984 Exemptions § 535.310 Marine terminal facilities agreement—exemption. (a) Marine terminal facilities agreement...

46 CFR 535.309 - Marine terminal services agreements-exemption.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 9 2010-10-01 2010-10-01 false Marine terminal services agreements-exemption. 535.309... COMMERCE OCEAN COMMON CARRIER AND MARINE TERMINAL OPERATOR AGREEMENTS SUBJECT TO THE SHIPPING ACT OF 1984 Exemptions § 535.309 Marine terminal services agreements—exemption. (a) Marine terminal services agreement...

46 CFR 535.309 - Marine terminal services agreements-exemption.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 9 2014-10-01 2014-10-01 false Marine terminal services agreements-exemption. 535.309... COMMERCE OCEAN COMMON CARRIER AND MARINE TERMINAL OPERATOR AGREEMENTS SUBJECT TO THE SHIPPING ACT OF 1984 Exemptions § 535.309 Marine terminal services agreements—exemption. (a) Marine terminal services agreement...

46 CFR 535.310 - Marine terminal facilities agreement-exemption.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 9 2014-10-01 2014-10-01 false Marine terminal facilities agreement-exemption. 535.310... COMMERCE OCEAN COMMON CARRIER AND MARINE TERMINAL OPERATOR AGREEMENTS SUBJECT TO THE SHIPPING ACT OF 1984 Exemptions § 535.310 Marine terminal facilities agreement—exemption. (a) Marine terminal facilities agreement...

46 CFR 535.309 - Marine terminal services agreements-exemption.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 9 2012-10-01 2012-10-01 false Marine terminal services agreements-exemption. 535.309... COMMERCE OCEAN COMMON CARRIER AND MARINE TERMINAL OPERATOR AGREEMENTS SUBJECT TO THE SHIPPING ACT OF 1984 Exemptions § 535.309 Marine terminal services agreements—exemption. (a) Marine terminal services agreement...

46 CFR 535.310 - Marine terminal facilities agreement-exemption.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 9 2013-10-01 2013-10-01 false Marine terminal facilities agreement-exemption. 535.310... COMMERCE OCEAN COMMON CARRIER AND MARINE TERMINAL OPERATOR AGREEMENTS SUBJECT TO THE SHIPPING ACT OF 1984 Exemptions § 535.310 Marine terminal facilities agreement—exemption. (a) Marine terminal facilities agreement...

14 CFR 158.85 - Termination of authority to impose PFC's.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Termination of authority to impose PFC's... TRANSPORTATION (CONTINUED) AIRPORTS PASSENGER FACILITY CHARGES (PFC'S) Termination § 158.85 Termination of authority to impose PFC's. (a) The FAA begins proceedings to terminate the public agency's authority to...

14 CFR 158.85 - Termination of authority to impose PFC's.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Termination of authority to impose PFC's... TRANSPORTATION (CONTINUED) AIRPORTS PASSENGER FACILITY CHARGES (PFC'S) Termination § 158.85 Termination of authority to impose PFC's. (a) The FAA begins proceedings to terminate the public agency's authority to...

14 CFR 158.85 - Termination of authority to impose PFC's.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Termination of authority to impose PFC's... TRANSPORTATION (CONTINUED) AIRPORTS PASSENGER FACILITY CHARGES (PFC'S) Termination § 158.85 Termination of authority to impose PFC's. (a) The FAA begins proceedings to terminate the public agency's authority to...

14 CFR 158.85 - Termination of authority to impose PFC's.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Termination of authority to impose PFC's... TRANSPORTATION (CONTINUED) AIRPORTS PASSENGER FACILITY CHARGES (PFC'S) Termination § 158.85 Termination of authority to impose PFC's. (a) The FAA begins proceedings to terminate the public agency's authority to...

14 CFR 158.85 - Termination of authority to impose PFC's.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Termination of authority to impose PFC's... TRANSPORTATION (CONTINUED) AIRPORTS PASSENGER FACILITY CHARGES (PFC'S) Termination § 158.85 Termination of authority to impose PFC's. (a) The FAA begins proceedings to terminate the public agency's authority to...

Fail-safe transcription termination: Because one is never enough

PubMed Central

Lemay, Jean-François; Bachand, François

2015-01-01

Termination of RNA polymerase II (RNAPII) transcription is a fundamental step of gene expression that involves the release of the nascent transcript and dissociation of RNAPII from the DNA template. As transcription termination is intimately linked to RNA 3′ end processing, termination pathways have a key decisive influence on the fate of the transcribed RNA. Quite remarkably, when reaching the 3′ end of genes, a substantial fraction of RNAPII fail to terminate transcription, requiring the contribution of alternative or “fail-safe” mechanisms of termination to release the polymerase. This point of view covers redundant mechanisms of transcription termination and how they relate to conventional termination models. In particular, we expand on recent findings that propose a reverse torpedo model of termination, in which the 3′5′ exonucleolytic activity of the RNA exosome targets transcription events associated with paused and backtracked RNAPII. PMID:26273910

Saccharomyces cerevisiae RNA Polymerase I Terminates Transcription at the Reb1 Terminator In Vivo

PubMed Central

Reeder, Ronald H.; Guevara, Palmira; Roan, Judith G.

1999-01-01

We have mapped transcription termination sites for RNA polymerase I in the yeast Saccharomyces cerevisiae. S1 nuclease mapping shows that the primary terminator is the Reb1p terminator located at +93 downstream of the 3′ end of 25S rRNA. Reverse transcription coupled with quantitative PCR shows that approximately 90% of all transcripts terminate at this site. Transcripts which read through the +93 site quantitatively terminate at a fail-safe terminator located further downstream at +250. Inactivation of Rnt1p (an RNase III involved in processing the 3′ end of 25S rRNA) greatly stabilizes transcripts extending to both sites and increases readthrough at the +93 site. In vivo assay of mutants of the Reb1p terminator shows that this site operates in vivo by the same mechanism as has previously been delineated through in vitro studies. PMID:10523625

Termination of DNA replication forks: "Breaking up is hard to do".

PubMed

Bailey, Rachael; Priego Moreno, Sara; Gambus, Agnieszka

2015-01-01

To ensure duplication of the entire genome, eukaryotic DNA replication initiates from thousands of replication origins. The replication forks move through the chromatin until they encounter forks from neighboring origins. During replication fork termination forks converge, the replisomes disassemble and topoisomerase II resolves the daughter DNA molecules. If not resolved efficiently, terminating forks result in genomic instability through the formation of pathogenic structures. Our recent findings shed light onto the mechanism of replisome disassembly upon replication fork termination. We have shown that termination-specific polyubiquitylation of the replicative helicase component - Mcm7, leads to dissolution of the active helicase in a process dependent on the p97/VCP/Cdc48 segregase. The inhibition of terminating helicase disassembly resulted in a replication termination defect. In this extended view we present hypothetical models of replication fork termination and discuss remaining and emerging questions in the DNA replication termination field.

Losing a father all over again: the termination of an analysis of an adolescent boy suffering from father loss.

PubMed

Sugarman, Alan

2010-08-01

Criteria for beginning and conducting the termination phase of psychoanalysis have provoked debate and confusion from the early days of psychoanalysis. Gabbard (2009) has recently pointed to the field's tendency to cling to idealized versions of these criteria as a way to deal with disagreements. The situation becomes more complicated for child and adolescent psychoanalysts because their patients are in the midst of a developmental process at the very time they are engaged in a psychoanalytic process. The termination phase of an adolescent male suffering from father loss is presented in depth in order to provide clinical data toward further consideration of the vexing questions surrounding termination in psychoanalysis. His termination is used to examine the relative importance of losing the analyst as a transference object as against a developmental object; the meaning of action during termination; the complicating role of trauma vis-à-vis termination; and the importance of the post-termination phase of analysis. It is suggested that his termination phase demonstrates that a "good enough" termination involves the development of a self-analyzing capacity that continues to evolve and develop after termination.

Anatomical Variability in the Termination of the Basilar Artery in the Human Cadaveric Brain.

PubMed

Gunnal, Sandhya; Farooqui, Mujeebuddin; Wabale, Rajendra

2015-01-01

The basilar artery (BA) is the prominent median vessel of the vertebrobasilar circulation and usually terminates into two posterior cerebral arteries forming the posterior angle of the Circle of Willis (CW). To tackle different variations of CW, basilar artery acts as a guideline for neuroradiologists and neurosurgeons. Basilar termination is the most frequent site of aneurysm. Abnormalities at the site of termination may compress the oculomotor nerve. Variations at the termination may complicate surgeries at the base of brain. The present study aims to add to the knowledge regarding the termination pattern of the BA. 170 BA terminations were studied. Morphological variations in the termination pattern were noted. Frequency of variations in termination patterns was recorded. Dimensions of BA were measured. Data were analyzed. Morphological variations in termination were seen in 17.64%. Bifurcation, Trifurcation, Quadrifurcation, Pentafurcation and Nonfurcation of BA was seen in 82.35%, 5.29%, 5.88%, 3.52% and 2.94% respectively. BA associated with aneurysm and Fenestration was seen in 3.52% and 1.17% respectively. Mean length and diameter of BA was 30.27 mm and 4.8 mm respectively. Awareness of these anatomical variations in termination patterns of BA is important in neurovascular procedures.

A systematic assessment of drought termination in the United Kingdom

NASA Astrophysics Data System (ADS)

Parry, Simon; Wilby, Robert L.; Prudhomme, Christel; Wood, Paul J.

2016-10-01

Drought termination can be associated with dramatic transitions from drought to flooding. Greater attention may be given to these newsworthy and memorable events, but drought terminations that proceed gradually also pose challenges for water resource managers. This paper defines drought termination as a distinctive phase of the event. Using observed river flow records for 52 UK catchments, a more systematic and objective approach for detecting drought terminations is demonstrated. The parameters of the approach are informed by a sensitivity analysis that ensures a focus on terminations of multi-season to multi-year droughts. The resulting inventory of 467 drought terminations provides an unprecedented historical perspective on this phenomenon in the UK. Nationally and regionally coherent drought termination events are identifiable, although their characteristics vary both between and within major episodes. Contrasting drought termination events in 1995-1998 and 2009-2012 are examined in greater depth. The data are also used to assess potential linkages between metrics of drought termination and catchment properties. The duration of drought termination is moderately negatively correlated with elevation (rs = -0.47) and catchment average rainfall (rs = -0.42), suggesting that wetter catchments in upland areas of the UK tend to experience shorter drought terminations. More urbanized catchments tend to have gradual drought terminations (contrary to expectations of flashy hydrological response in such areas), although this may also reflect the type of catchments typical of lowland England. Significant correlations are found between the duration of the drought development phase and both the duration (rs = -0.29) and rate (rs = 0.28) of drought termination. This suggests that prolonged drought development phases tend to be followed by shorter and more abrupt drought terminations. The inventory helps to place individual events within a long-term context. The drought termination phase in 2009-2012 was, at the time, regarded as exceptional in terms of magnitude and spatial footprint, but the Thames river flow record identifies several comparable events before 1930. The chronology could, in due course, provide a basis for exploring the complex drivers, long-term variability, and impacts of drought termination events.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shi, Zhiwei; Walker, Amy V., E-mail: amy.walker@utdallas.edu

The room temperature atomic layerlike deposition (ALLD) of ZnS on functionalized self-assembled monolayers (SAMs) was investigated, using diethyl zinc (DEZ) and in situ generated H{sub 2}S as reactants. Depositions on SAMs with three different terminal groups, –CH{sub 3,} –OH, and –COOH, were studied. It was found that the reaction of DEZ with the SAM terminal group is critical in determining the film growth rate. Little or no deposition is observed on –CH{sub 3} terminated SAMs because DEZ does not react with the methyl terminal group. ZnS does deposit on both –OH and –COOH terminated SAMs, but the grow rate onmore » –COOH terminated SAMs is ∼10% lower per cycle than on –OH terminated SAMs. DEZ reacts with the hydroxyl group on –OH terminated SAMs, while on –COOH terminated SAMs it reacts with both the hydroxyl and carbonyl bonds of the terminal groups. The carbonyl reaction is found to lead to the formation of ketones rather than deposition of ZnS, lowering the growth rate on –COOH terminated SAMs. SIMS spectra show that both –OH and –COOH terminated SAMs are covered by the deposited ZnS layer after five ALLD cycles. In contrast to ZnO ALLD where the composition of the film differs for the first few layers on –COOH and –OH terminated SAMs, the deposited film composition is the same for both –COOH and –OH terminated SAMs. The deposited film is found to be Zn-rich, suggesting that the reaction of H{sub 2}S with the Zn-surface adduct may be incomplete.« less

Pregnancy termination in Matlab, Bangladesh: trends and correlates of use of safer and less-safe methods.

PubMed

DaVanzo, Julie; Rahman, Mizanur

2014-09-01

Menstrual regulation (MR), a relatively safe form of pregnancy termination, is legal in Bangladesh during the early stages of pregnancy. However, little is known about the factors associated with whether women who terminate pregnancies choose this method or a less-safe one. Data from the Matlab Demographic Surveillance System on 122,691 pregnancies-5,221 (4.3%) of which were terminated-were used to examine trends between 1989 and 2008 in termination and in use of safer methods (MR or dilation and curettage) and less-safe (all other) methods of pregnancy termination. Logistic and multinomial logistic regressions were used to assess factors associated with whether women terminate pregnancies and whether they use safer methods. Sixty-seven percent of pregnancy terminations were by safer methods and 33% by less-safe means. The proportion of pregnancies that were terminated increased between 1989 and 2008; this increase was entirely due to increased use of safer methods. Women younger than 18 and those 25 or older were more likely than women aged 20-24 to terminate their pregnancies (odds ratios ranged from 1.5 among women aged 16-17 or 25-29 to 26.1 among those aged 45 or older). Among women who terminated their pregnancies, those aged 25-44 were more likely than those aged 20-24 to use a safer method. Compared with women who had no formal education, those with some education were more likely to terminate their pregnancies and to do so using safer methods. A growing proportion of pregnancies in Matlab are terminated, and these terminations are increasingly done using safer methods.

Loss of corticostriatal and thalamostriatal synaptic terminals precedes striatal projection neuron pathology in heterozygous Q140 Huntington's disease mice.

PubMed

Deng, Y P; Wong, T; Bricker-Anthony, C; Deng, B; Reiner, A

2013-12-01

Motor slowing, forebrain white matter loss, and striatal shrinkage have been reported in premanifest Huntington's disease (HD) prior to overt striatal neuron loss. We carried out detailed LM and EM studies in a genetically precise HD mimic, heterozygous Q140 HD knock-in mice, to examine the possibility that loss of corticostriatal and thalamostriatal terminals prior to striatal neuron loss underlies these premanifest HD abnormalities. In our studies, we used VGLUT1 and VGLUT2 immunolabeling to detect corticostriatal and thalamostriatal (respectively) terminals in dorsolateral (motor) striatum over the first year of life, prior to striatal projection neuron pathology. VGLUT1+ axospinous corticostriatal terminals represented about 55% of all excitatory terminals in striatum, and VGLUT2+ axospinous thalamostriatal terminals represented about 35%, with VGLUT1+ and VGLUT2+ axodendritic terminals accounting for the remainder. In Q140 mice, a significant 40% shortfall in VGLUT2+ axodendritic thalamostriatal terminals and a 20% shortfall in axospinous thalamostriatal terminals were already observed at 1 month of age, but VGLUT1+ terminals were normal in abundance. The 20% deficiency in VGLUT2+ thalamostriatal axospinous terminals persisted at 4 and 12 months in Q140 mice, and an additional 30% loss of VGLUT1+ corticostriatal terminals was observed at 12 months. The early and persistent deficiency in thalamostriatal axospinous terminals in Q140 mice may reflect a development defect, and the impoverishment of this excitatory drive to striatum may help explain early motor defects in Q140 mice and in premanifest HD. The loss of corticostriatal terminals at 1 year in Q140 mice is consistent with prior evidence from other mouse models of corticostriatal disconnection early during progression, and can explain both the measurable bradykinesia and striatal white matter loss in late premanifest HD. © 2013.

42 CFR 422.512 - Termination of contract by the MA organization.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 42 Public Health 3 2014-10-01 2014-10-01 false Termination of contract by the MA organization. 422... Procedures and Contracts for Medicare Advantage Organizations § 422.512 Termination of contract by the MA organization. (a) Cause for termination. The MA organization may terminate the MA contract if CMS fails to...

42 CFR 422.512 - Termination of contract by the MA organization.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 3 2010-10-01 2010-10-01 false Termination of contract by the MA organization. 422... Contracts for Medicare Advantage Organizations § 422.512 Termination of contract by the MA organization. (a) Cause for termination. The MA organization may terminate the MA contract if CMS fails to substantially...

42 CFR 422.512 - Termination of contract by the MA organization.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 3 2011-10-01 2011-10-01 false Termination of contract by the MA organization. 422... Contracts for Medicare Advantage Organizations § 422.512 Termination of contract by the MA organization. (a) Cause for termination. The MA organization may terminate the MA contract if CMS fails to substantially...

42 CFR 422.512 - Termination of contract by the MA organization.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 42 Public Health 3 2012-10-01 2012-10-01 false Termination of contract by the MA organization. 422... Procedures and Contracts for Medicare Advantage Organizations § 422.512 Termination of contract by the MA organization. (a) Cause for termination. The MA organization may terminate the MA contract if CMS fails to...

42 CFR 422.512 - Termination of contract by the MA organization.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 42 Public Health 3 2013-10-01 2013-10-01 false Termination of contract by the MA organization. 422... Procedures and Contracts for Medicare Advantage Organizations § 422.512 Termination of contract by the MA organization. (a) Cause for termination. The MA organization may terminate the MA contract if CMS fails to...

42 CFR § 512.905 - Termination of the CR incentive payment model.

Code of Federal Regulations, 2010 CFR

2017-10-01

... 42 Public Health 5 2017-10-01 2017-10-01 false Termination of the CR incentive payment model. Â... HEALTH AND HUMAN SERVICES (CONTINUED) HEALTH CARE INFRASTRUCTURE AND MODEL PROGRAMS EPISODE PAYMENT MODEL Model Termination § 512.905 Termination of the CR incentive payment model. CMS may terminate the CR...

42 CFR 421.205 - Termination by the Secretary.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 42 Public Health 3 2014-10-01 2014-10-01 false Termination by the Secretary. 421.205 Section 421... (CONTINUED) MEDICARE PROGRAM (CONTINUED) MEDICARE CONTRACTING Carriers § 421.205 Termination by the Secretary. (a) Cause for termination. The Secretary may terminate a contract with a carrier at any time if he or...

Terminator Detection by Support Vector Machine Utilizing aStochastic Context-Free Grammar

DOE Office of Scientific and Technical Information (OSTI.GOV)

Francis-Lyon, Patricia; Cristianini, Nello; Holbrook, Stephen

2006-12-30

A 2-stage detector was designed to find rho-independent transcription terminators in the Escherichia coli genome. The detector includes a Stochastic Context Free Grammar (SCFG) component and a Support Vector Machine (SVM) component. To find terminators, the SCFG searches the intergenic regions of nucleotide sequence for local matches to a terminator grammar that was designed and trained utilizing examples of known terminators. The grammar selects sequences that are the best candidates for terminators and assigns them a prefix, stem-loop, suffix structure using the Cocke-Younger-Kasaami (CYK) algorithm, modified to incorporate energy affects of base pairing. The parameters from this inferred structure aremore » passed to the SVM classifier, which distinguishes terminators from non-terminators that score high according to the terminator grammar. The SVM was trained with negative examples drawn from intergenic sequences that include both featureless and RNA gene regions (which were assigned prefix, stem-loop, suffix structure by the SCFG), so that it successfully distinguishes terminators from either of these. The classifier was found to be 96.4% successful during testing.« less

The morphological characteristics of corticostriatal and thalamostriatal neurons and their intrastriatal terminals in rats.

PubMed

Liu, Bingbing; Ouyang, Lisi; Mu, Shuhua; Zhu, Yaxi; Li, Keyi; Zhan, Mali; Liu, Zongwei; Jia, Yu; Lei, Wanlong

2011-11-01

The glutamatergic projection from the cerebral cortex and the thalamus extensively innervates the neostriatal neurons. However, some conflicts in the published literatures about cortical and thalamic intrastriatal synaptic terminals still need to be resolved. The present study intends to further elucidate the morphological characteristics of these two types of the terminals and their neurons. The corticostriatal and thalamostriatal terminals were immunolabeled for vesicular glutamate transporter type 1 (VGluT1) and 2 (VGluT2), respectively, and their neurons were retrograde labeled by biotinylated dextran amine 3,000 molecular weight (BDA3k) injection into the dorsolateral striatum of rats. The characteristics of the corticostriatal and thalamostriatal terminals were observed at the LM and EM levels, and the data were statistically analyzed with SPSS10.0 software. We observed that 63.53% of VGluT1+ terminals synapsed on dendritic spines, which was different from VGluT2+ terminals with the equal percentage of synapses on spines and dendrites (14.88 and 17.86%, respectively). Notably, VGluT1+ axospinous synaptic terminals were remarkably larger than VGluT2+ axospinous synaptic terminals. Terminal size-frequency distribution analysis showed that VGluT1+ terminals were within the size ranges of 0.4-0.5 and 0.8-0.9 μm, and VGluT2+ terminals were in the ranges of 0.4-0.5 and 0.6-0.7 μm. Perforated-postsynaptic densities (-PSDs) were more frequently found in VGluT1+ axospinous synaptic terminals than in VGluT2+ axospinous terminals. Furthermore, BDA3k-labeled corticostrital neurons were larger in perikaryal diameter than the thalamostriatal neurons, and they were also categorized as the two main populations based on their size-frequency distribution. The morphological characteristics of corticostriatal and thalamostriatal terminals and neurons have implications for understanding the roles of synaptic plasticity in adaptive motor control by the basal ganglia, and they have facilitations for understanding the complexities of basal ganglia function.

The HSP terminator of Arabidopsis thaliana increases gene expression in plant cells.

PubMed

Nagaya, Shingo; Kawamura, Kazue; Shinmyo, Atsuhiko; Kato, Ko

2010-02-01

To express a foreign gene in plants effectively, a good expression system is required. Here we describe the identification of a transcriptional terminator that supports increased levels of expression. The terminators of several Arabidopsis genes were examined in transfected Arabidopsis T87 protoplasts. The heat shock protein 18.2 (HSP) terminator was the most effective in supporting increased levels of expression. The HSP terminator increases mRNA levels of both transiently and stably expressed transgenes approximately 2-fold more than the NOS (nopaline synthase) terminator. When combined with the HSP terminator, a translational enhancer increased gene expression levels approximately 60- to 100-fold in transgenic plants.

MSAT aeronautical mobile satellite communications terminal development

NASA Technical Reports Server (NTRS)

Sutherland, C. A.; Sydor, J. T.

1995-01-01

CAL has undertaken the development of a new aeronautical mobile terminal for the North American MSAT market. The terminal is to meet the MSAT standard and is aimed in particular at the 300,000 general aviation and business aircraft in North America. The terminals are therefore relatively low cost and small in size when compared to those currently being produced for larger airline aircraft. The terminal incorporates a top mounted mechanical steered antenna and a unique antenna steering subsystem. An overview of the terminal design is presented.

Evolutionary Technique for Automated Synthesis of Electronic Circuits

NASA Technical Reports Server (NTRS)

Stoica, Adrian (Inventor); Salazar-Lazaro, Carlos Harold (Inventor)

2007-01-01

An evolvable circuit includes a plurality of reconfigurable switches, a plurality of transistors within a region of the circuit, the plurality of transistors having terminals, the plurality of transistors being coupled between a power source terminal and a power sink terminal so as to be capable of admitting power between the power source terminal and the power sink terminal, the plurality of transistors being coupled so that every transistor to transistor terminal coupling within the region of the circuit comprises a reconfigurable switch.

33 CFR 165.1198 - Safety zone; Military Ocean Terminal Concord Safety Zone, Suisun Bay, Military Ocean Terminal...

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Safety zone; Military Ocean Terminal Concord Safety Zone, Suisun Bay, Military Ocean Terminal Concord, CA. 165.1198 Section 165.1198... Limited Access Areas Eleventh Coast Guard District § 165.1198 Safety zone; Military Ocean Terminal Concord...

33 CFR 165.1198 - Safety zone; Military Ocean Terminal Concord Safety Zone, Suisun Bay, Military Ocean Terminal...

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Safety zone; Military Ocean Terminal Concord Safety Zone, Suisun Bay, Military Ocean Terminal Concord, CA. 165.1198 Section 165.1198... Limited Access Areas Eleventh Coast Guard District § 165.1198 Safety zone; Military Ocean Terminal Concord...

42 CFR § 512.900 - Termination of an episode payment model.

Code of Federal Regulations, 2010 CFR

2017-10-01

... 42 Public Health 5 2017-10-01 2017-10-01 false Termination of an episode payment model. § 512.900... SERVICES (CONTINUED) HEALTH CARE INFRASTRUCTURE AND MODEL PROGRAMS EPISODE PAYMENT MODEL Model Termination § 512.900 Termination of an episode payment model. CMS may terminate any EPM for reasons including but...

78 FR 5717 - Safety Zone; Military Ocean Terminal Concord Safety Zone, Suisun Bay, Military Ocean Terminal...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-28

...-AA00 Safety Zone; Military Ocean Terminal Concord Safety Zone, Suisun Bay, Military Ocean Terminal... Guard is establishing a safety zone in the navigable waters of Suisun Bay near Military Ocean Terminal Concord, CA in support of military onload and offload operations. This safety zone is established to...

Airborne Satcom Terminal Research at NASA Glenn

NASA Technical Reports Server (NTRS)

Hoder, Doug; Zakrajsek, Robert

2002-01-01

NASA Glenn has constructed an airborne Ku-band satellite terminal, which provides wideband full-duplex ground-aircraft communications. The terminal makes use of novel electronically-steered phased array antennas and provides IP connectivity to and from the ground. The satcom terminal communications equipment may be easily changed whenever a new configuration is required, enhancing the terminal's versatility.

Prediction of Transcriptional Terminators in Bacillus subtilis and Related Species

PubMed Central

de Hoon, Michiel J. L.; Makita, Yuko; Nakai, Kenta; Miyano, Satoru

2005-01-01

In prokaryotes, genes belonging to the same operon are transcribed in a single mRNA molecule. Transcription starts as the RNA polymerase binds to the promoter and continues until it reaches a transcriptional terminator. Some terminators rely on the presence of the Rho protein, whereas others function independently of Rho. Such Rho-independent terminators consist of an inverted repeat followed by a stretch of thymine residues, allowing us to predict their presence directly from the DNA sequence. Unlike in Escherichia coli, the Rho protein is dispensable in Bacillus subtilis, suggesting a limited role for Rho-dependent termination in this organism and possibly in other Firmicutes. We analyzed 463 experimentally known terminating sequences in B. subtilis and found a decision rule to distinguish Rho-independent transcriptional terminators from non-terminating sequences. The decision rule allowed us to find the boundaries of operons in B. subtilis with a sensitivity and specificity of about 94%. Using the same decision rule, we found an average sensitivity of 94% for 57 bacteria belonging to the Firmicutes phylum, and a considerably lower sensitivity for other bacteria. Our analysis shows that Rho-independent termination is dominant for Firmicutes in general, and that the properties of the transcriptional terminators are conserved. Terminator prediction can be used to reliably predict the operon structure in these organisms, even in the absence of experimentally known operons. Genome-wide predictions of Rho-independent terminators for the 57 Firmicutes are available in the Supporting Information section. PMID:16110342

C-terminal domains of bacterial proteases: structure, function and the biotechnological applications.

PubMed

Huang, J; Wu, C; Liu, D; Yang, X; Wu, R; Zhang, J; Ma, C; He, H

2017-01-01

C-terminal domains widely exist in the C-terminal region of multidomain proteases. As a β-sandwich domain in multidomain protease, the C-terminal domain plays an important role in proteolysis including regulation of the secretory process, anchoring and swelling the substrate molecule, presenting as an inhibitor for the preprotease and adapting the protein structural flexibility and stability. In this review, the diversity, structural characteristics and biological function of C-terminal protease domains are described. Furthermore, the application prospects of C-terminal domains, including polycystic kidney disease, prepeptidase C-terminal and collagen-binding domain, in the area of medicine and biological artificial materials are also discussed. © 2016 The Society for Applied Microbiology.

Rapid Acquisition of Choice and Timing and the Provenance of the Terminal-Link Effect

ERIC Educational Resources Information Center

Kyonka, Elizabeth G. E.; Grace, Randolph C.

2010-01-01

Eight pigeons responded in a concurrent-chains procedure in which terminal-link schedules changed pseudorandomly across sessions. Pairs of terminal-link delays either summed to 15 s or to 45 s. Across sessions, the location of the shorter terminal link changed according to a pseudorandom binary sequence. On some terminal links, food was withheld…

47 CFR 25.134 - Licensing provisions of Very Small Aperture Terminal (VSAT) and C-band Small Aperture Terminal...

Code of Federal Regulations, 2011 CFR

2011-10-01

... Terminal (VSAT) and C-band Small Aperture Terminal (CSAT) networks. 25.134 Section 25.134 Telecommunication...) and C-band Small Aperture Terminal (CSAT) networks. (a)(1) VSAT networks operating in the 12/14 GHz bands. All applications for digital VSAT networks granted on or before September 15, 2005, with a...

47 CFR 25.134 - Licensing provisions of Very Small Aperture Terminal (VSAT) and C-band Small Aperture Terminal...

Code of Federal Regulations, 2010 CFR

2010-10-01

... Terminal (VSAT) and C-band Small Aperture Terminal (CSAT) networks. 25.134 Section 25.134 Telecommunication...) and C-band Small Aperture Terminal (CSAT) networks. (a)(1) VSAT networks operating in the 12/14 GHz bands. All applications for digital VSAT networks granted on or before September 15, 2005, with a...

Different domains of the murine RNA polymerase I-specific termination factor mTTF-I serve distinct functions in transcription termination.

PubMed

Evers, R; Smid, A; Rudloff, U; Lottspeich, F; Grummt, I

1995-03-15

Termination of mouse ribosomal gene transcription by RNA polymerase I (Pol I) requires the specific interaction of a DNA binding protein, mTTF-I, with an 18 bp sequence element located downstream of the rRNA coding region. Here we describe the molecular cloning and functional characterization of the cDNA encoding this transcription termination factor. Recombinant mTTF-I binds specifically to the murine terminator elements and terminates Pol I transcription in a reconstituted in vitro system. Deletion analysis has defined a modular structure of mTTF-I comprising a dispensable N-terminal half, a large C-terminal DNA binding region and an internal domain which is required for transcription termination. Significantly, the C-terminal region of mTTF-I reveals striking homology to the DNA binding domains of the proto-oncogene c-Myb and the yeast transcription factor Reb1p. Site-directed mutagenesis of one of the tryptophan residues that is conserved in the homology region of c-Myb, Reb1p and mTTF-I abolishes specific DNA binding, a finding which underscores the functional relevance of these residues in DNA-protein interactions.

Different domains of the murine RNA polymerase I-specific termination factor mTTF-I serve distinct functions in transcription termination.

PubMed Central

Evers, R; Smid, A; Rudloff, U; Lottspeich, F; Grummt, I

1995-01-01

Termination of mouse ribosomal gene transcription by RNA polymerase I (Pol I) requires the specific interaction of a DNA binding protein, mTTF-I, with an 18 bp sequence element located downstream of the rRNA coding region. Here we describe the molecular cloning and functional characterization of the cDNA encoding this transcription termination factor. Recombinant mTTF-I binds specifically to the murine terminator elements and terminates Pol I transcription in a reconstituted in vitro system. Deletion analysis has defined a modular structure of mTTF-I comprising a dispensable N-terminal half, a large C-terminal DNA binding region and an internal domain which is required for transcription termination. Significantly, the C-terminal region of mTTF-I reveals striking homology to the DNA binding domains of the proto-oncogene c-Myb and the yeast transcription factor Reb1p. Site-directed mutagenesis of one of the tryptophan residues that is conserved in the homology region of c-Myb, Reb1p and mTTF-I abolishes specific DNA binding, a finding which underscores the functional relevance of these residues in DNA-protein interactions. Images PMID:7720715

Multigene Expression In Vivo: Supremacy of Large Versus Small Terminators for T7 RNA Polymerase

PubMed Central

Du, Liping; Villarreal, Seth; Forster, Anthony C.

2012-01-01

Designing and building multigene constructs is commonplace in synthetic biology. Yet functional successes at first attempts are rare because the genetic parts are not fully modular. In order to improve the modularity of transcription, we previously showed that transcription termination in vitro by bacteriophage T7 RNA polymerase could be made more efficient by substituting the standard, single, TΦ large (class I) terminator with adjacent copies of the Vesicular Stomatitis Virus (VSV) small (class II) terminator. However, in vitro termination at the downstream VSV terminator was less efficient than at the upstream VSV terminator, and multigene overexpression in vivo was complicated by unexpectedly inefficient VSV termination within E. coli cells. Here, we address hypotheses raised in that study by showing that VSV or preproparathyroid hormone (PTH) small terminators spaced further apart can work independently (i.e. more efficiently) in vitro, and that VSV and PTH terminations are severely inhibited in vivo. Surprisingly, the difference between class II terminator function in vivo versus in vitro is not due to differences in plasmid supercoiling, as supercoiling had a minimal effect on termination in vitro. We therefore turned to TΦ terminators for “BioBrick” synthesis of a pentameric gene construct suitable for overexpression in vivo. This indeed enabled coordinated overexpression and copurification of five His-tagged proteins using the first construct attempted, indicating that this strategy is more modular than other strategies. An application of this multigene overexpression and protein copurification method is demonstrated by supplying five of the six E. coli translation factors required for reconstitution of translation from a single cell line via copurification, greatly simplifying the reconstitution. PMID:22094962

Identified motor terminals in Drosophila larvae show distinct differences in morphology and physiology

NASA Technical Reports Server (NTRS)

Lnenicka, G. A.; Keshishian, H.

2000-01-01

In Drosophila, the type I motor terminals innervating the larval ventral longitudinal muscle fibers 6 and 7 have been the most popular preparation for combining synaptic studies with genetics. We have further characterized the normal morphological and physiological properties of these motor terminals and the influence of muscle size on terminal morphology. Using dye-injection and physiological techniques, we show that the two axons supplying these terminals have different innervation patterns: axon 1 innervates only muscle fibers 6 and 7, whereas axon 2 innervates all of the ventral longitudinal muscle fibers. This difference in innervation pattern allows the two axons to be reliably identified. The terminals formed by axons 1 and 2 on muscle fibers 6 and 7 have the same number of branches; however, axon 2 terminals are approximately 30% longer than axon 1 terminals, resulting in a corresponding greater number of boutons for axon 2. The axon 1 boutons are approximately 30% wider than the axon 2 boutons. The excitatory postsynaptic potential (EPSP) produced by axon 1 is generally smaller than that produced by axon 2, although the size distributions show considerable overlap. Consistent with vertebrate studies, there is a correlation between muscle fiber size and terminal size. For a single axon, terminal area and length, the number of terminal branches, and the number of boutons are all correlated with muscle fiber size, but bouton size is not. During prolonged repetitive stimulation, axon 2 motor terminals show synaptic depression, whereas axon 1 EPSPs facilitate. The response to repetitive stimulation appears to be similar at all motor terminals of an axon. Copyright 2000 John Wiley & Sons, Inc.

20 CFR 408.814 - Can you request termination of your SVB entitlement?

Code of Federal Regulations, 2013 CFR

2013-04-01

... CERTAIN WORLD WAR II VETERANS Suspensions and Terminations Termination § 408.814 Can you request.... When a termination request is filed, your SVB entitlement ends effective with the month following the...

20 CFR 408.814 - Can you request termination of your SVB entitlement?

Code of Federal Regulations, 2010 CFR

2010-04-01

... CERTAIN WORLD WAR II VETERANS Suspensions and Terminations Termination § 408.814 Can you request.... When a termination request is filed, your SVB entitlement ends effective with the month following the...

20 CFR 408.814 - Can you request termination of your SVB entitlement?

Code of Federal Regulations, 2011 CFR

2011-04-01

... CERTAIN WORLD WAR II VETERANS Suspensions and Terminations Termination § 408.814 Can you request.... When a termination request is filed, your SVB entitlement ends effective with the month following the...

20 CFR 408.814 - Can you request termination of your SVB entitlement?

Code of Federal Regulations, 2014 CFR

2014-04-01

... CERTAIN WORLD WAR II VETERANS Suspensions and Terminations Termination § 408.814 Can you request.... When a termination request is filed, your SVB entitlement ends effective with the month following the...

20 CFR 408.814 - Can you request termination of your SVB entitlement?

Code of Federal Regulations, 2012 CFR

2012-04-01

... CERTAIN WORLD WAR II VETERANS Suspensions and Terminations Termination § 408.814 Can you request.... When a termination request is filed, your SVB entitlement ends effective with the month following the...

Terminated Trials in the ClinicalTrials.gov Results Database: Evaluation of Availability of Primary Outcome Data and Reasons for Termination.

PubMed

Williams, Rebecca J; Tse, Tony; DiPiazza, Katelyn; Zarin, Deborah A

2015-01-01

Clinical trials that end prematurely (or "terminate") raise financial, ethical, and scientific concerns. The extent to which the results of such trials are disseminated and the reasons for termination have not been well characterized. A cross-sectional, descriptive study of terminated clinical trials posted on the ClinicalTrials.gov results database as of February 2013 was conducted. The main outcomes were to characterize the availability of primary outcome data on ClinicalTrials.gov and in the published literature and to identify the reasons for trial termination. Approximately 12% of trials with results posted on the ClinicalTrials.gov results database (905/7,646) were terminated. Most trials were terminated for reasons other than accumulated data from the trial (68%; 619/905), with an insufficient rate of accrual being the lead reason for termination among these trials (57%; 350/619). Of the remaining trials, 21% (193/905) were terminated based on data from the trial (findings of efficacy or toxicity) and 10% (93/905) did not specify a reason. Overall, data for a primary outcome measure were available on ClinicalTrials.gov and in the published literature for 72% (648/905) and 22% (198/905) of trials, respectively. Primary outcome data were reported on the ClinicalTrials.gov results database and in the published literature more frequently (91% and 46%, respectively) when the decision to terminate was based on data from the trial. Trials terminate for a variety of reasons, not all of which reflect failures in the process or an inability to achieve the intended goals. Primary outcome data were reported most often when termination was based on data from the trial. Further research is needed to identify best practices for disseminating the experience and data resulting from terminated trials in order to help ensure maximal societal benefit from the investments of trial participants and others involved with the study.

Loss of Corticostriatal and Thalamostriatal Synaptic Terminals Precedes Striatal Projection Neuron Pathology in Heterozygous Q140 Huntington’s Disease Mice

PubMed Central

Deng, Y.P.; Wong, T.; Bricker-Anthony, C.; Deng, B.; Reiner, A.

2013-01-01

Motor slowing, forebrain white matter loss, and striatal shrinkage have been reported in premanifest Huntington’s disease (HD) prior to overt striatal neuron loss. We carried out detailed LM and EM studies in a genetically precise HD mimic, heterozygous Q140 HD knock-in mice, to examine the possibility that loss of corticostriatal and thalamostriatal terminals prior to striatal neuron loss underlies these premanifest HD abnormalities. In our studies, we used VGLUT1 and VGLUT2 immunolabeling to detect corticostriatal and thalamostriatal (respectively) terminals in dorsolateral (motor) striatum over the first year of life, prior to striatal projection neuron pathology. VGLUT1+ axospinous corticostriatal terminals represented about 55% of all excitatory terminals in striatum, and VGLUT2+ axospinous thalamostriatal terminals represented about 35%, with VGLUT1+ and VGLUT2+ axodendritic terminals accounting for the remainder. In Q140 mice, a significant 40% shortfall in VGLUT2+ axodendritic thalamostriatal terminals and a 20% shortfall in axospinous thalamostriatal terminals was already observed at 1 month of age, but VGLUT1+ terminals were normal in abundance. The 20% deficiency in VGLUT2+ thalamostriatal axospinous terminals persisted at 4 and 12 months in Q140 mice, and an additional 30% loss of VGLUT1+ corticostriatal terminals was observed at 12 months. The early and persistent deficiency in thalamostriatal axospinous terminals in Q140 mice may reflect a development defect, and the impoverishment of this excitatory drive to striatum may help explain early motor defects in Q140 mice and in premanifest HD. The loss of corticostriatal terminals at 1 year in Q140 mice is consistent with prior evidence from other mouse models of corticostriatal disconnection early during progression, and can explain both the measurable bradykinesia and striatal white matter loss in late premanifest HD. PMID:23969239

RNA polymerase III mutants in TFIIFα-like C37 that cause terminator readthrough with no decrease in transcription output.

PubMed

Rijal, Keshab; Maraia, Richard J

2013-01-07

How eukaryotic RNA polymerases switch from elongation to termination is unknown. Pol III subunits Rpc53 and Rpc37 (C53/37) form a heterodimer homologous to TFIIFβ/α. C53/37 promotes efficient termination and together with C11 also mediates pol III recycling in vitro. We previously developed Schizosaccharomyces pombe strains that report on two pol III termination activities: RNA oligo(U) 3'-end cleavage, and terminator readthrough. We randomly mutagenized C53 and C37 and isolated many C37 mutants with terminator readthrough but no comparable C53 mutants. The majority of C37 mutants have strong phenotypes with up to 40% readthrough and map to a C-terminal tract previously localized near Rpc2p in the pol III active center while a minority represent a distinct class with weaker phenotype, less readthrough and 3'-oligo(U) lengthening. Nascent pre-tRNAs released from a terminator by C37 mutants have shorter 3'-oligo(U) tracts than in cleavage-deficient C11 double mutants indicating RNA 3'-end cleavage during termination. We asked whether termination deficiency affects transcription output in the mutants in vivo both by monitoring intron-containing nascent transcript levels and (14)C-uridine incorporation. Surprisingly, multiple termination mutants have no decrease in transcript output relative to controls. These data are discussed in context of current models of pol III transcription.

Tail-extension following the termination codon is critical for release of the nascent chain from membrane-bound ribosomes in a reticulocyte lysate cell-free system.

PubMed

Takahara, Michiyo; Sakaue, Haruka; Onishi, Yukiko; Yamagishi, Marifu; Kida, Yuichiro; Sakaguchi, Masao

2013-01-11

Nascent chain release from membrane-bound ribosomes by the termination codon was investigated using a cell-free translation system from rabbit supplemented with rough microsomal membrane vesicles. Chain release was extremely slow when mRNA ended with only the termination codon. Tail extension after the termination codon enhanced the release of the nascent chain. Release reached plateau levels with tail extension of 10 bases. This requirement was observed with all termination codons: TAA, TGA and TAG. Rapid release was also achieved by puromycin even in the absence of the extension. Efficient translation termination cannot be achieved in the presence of only a termination codon on the mRNA. Tail extension might be required for correct positioning of the termination codon in the ribosome and/or efficient recognition by release factors. Copyright © 2012. Published by Elsevier Inc.

Method of making hermetic seals for hermetic terminal assemblies

DOEpatents

Hsu, John S.; Marlino, Laura D.; Ayers, Curtis W.

2010-04-13

This invention teaches methods of making a hermetic terminal assembly comprising the steps of: inserting temporary stops, shims and jigs on the bottom face of a terminal assembly thereby blocking assembly core open passageways; mounting the terminal assembly inside a vacuum chamber using a temporary assembly perimeter seal and flange or threaded assembly interfaces; mixing a seal admixture and hardener in a mixer conveyor to form a polymer seal material; conveying the polymer seal material into a polymer reservoir; feeding the polymer seal material from the reservoir through a polymer outlet valve and at least one polymer outlet tube into the terminal assembly core thereby filling interstitial spaces in the core adjacent to service conduits, temporary stop, and the terminal assembly casing; drying the polymer seal material at room temperature thereby hermetically sealing the core of the terminal assembly; removing the terminal assembly from the vacuum chamber, and; removing the temporary stops, shims.

Joint Modeling Approach for Semicompeting Risks Data with Missing Nonterminal Event Status

PubMed Central

Hu, Chen; Tsodikov, Alex

2014-01-01

Semicompeting risks data, where a subject may experience sequential non-terminal and terminal events, and the terminal event may censor the non-terminal event but not vice versa, are widely available in many biomedical studies. We consider the situation when a proportion of subjects’ non-terminal events is missing, such that the observed data become a mixture of “true” semicompeting risks data and partially observed terminal event only data. An illness-death multistate model with proportional hazards assumptions is proposed to study the relationship between non-terminal and terminal events, and provide covariate-specific global and local association measures. Maximum likelihood estimation based on semiparametric regression analysis is used for statistical inference, and asymptotic properties of proposed estimators are studied using empirical process and martingale arguments. We illustrate the proposed method with simulation studies and data analysis of a follicular cell lymphoma study. PMID:24430204

Hsp90 N- and C-terminal double inhibition synergistically suppresses Bcr-Abl-positive human leukemia cells

PubMed Central

Chen, Xianling; Chen, Xiaole; Li, Ding; Fan, Yingjuan; Xu, Jianhua; Chen, Yuanzhong; Wu, Lixian

2017-01-01

Heat shock protein 90 (Hsp90) contains amino (N)–terminal domain, carboxyl(C)-terminal domain, and middle domains, which activate Hsp90 chaperone function cooperatively in tumor cells. One terminal occupancy might influence another terminal binding with inhibitor. The Bcr-Abl kinase is one of the Hsp90 clients implicated in the pathogenesis of chronic myeloid leukemia (CML). Present studies demonstrate that double inhibition of the N- and C-terminal termini can disrupt Hsp90 chaperone function synergistically, but not antagonistically, in Bcr-Abl-positive human leukemia cells. Furthermore, both the N-terminal inhibitor 17-AAG and the C-terminal inhibitor cisplatin (CP) have the capacity to suppress progenitor cells; however, only CP is able to inhibit leukemia stem cells (LSCs) significantly, which implies that the combinational treatment is able to suppress human leukemia in different mature states. PMID:28036294

Solar Terminator Waves in the Ionosphere Measured by the Wallops Island, VA Dynasonde

NASA Astrophysics Data System (ADS)

Zabotin, N. A.; Song, H.; Bullett, T. W.

2017-12-01

Solar terminator represents a unique source of atmospheric waves possessing of near-ideal coherent properties: its geometry and magnitude of the impact changes very little from day to day. This feature has been used in [Forbes et al., GRL, 2008] to obtain "snapshots" of terminator waves in the neutral atmosphere at the altitude 400 km by averaging CHAMP accelerometer data over relatively long sequences of the satellite passes. The results were represented in the geographic latitude vs local time coordinates. We apply a similar approach averaging time series of Wallops Island, VA Dynasonde Doppler data to obtain "snapshots" of terminator waves in the ionosphere in the true altitude vs local "terminator time" coordinates. The averaging is performed independently for every month of the yearlong observation period from May 2013 to April 2014. The altitude range covered is 90 km to 400 km with 2 km resolution, representing the entire bottom-side ionosphere. Individual local time segments used for the averaging were 12 hours long and all centered at the times of the sunrise or sunset terminator passing at every specific altitude. This procedure effectively suppresses all kinds of incoherent wave activity and allows one to reveal the perturbation phenomenon mainly caused by the solar terminator. This is an important advantage of this technique compared to multiple "terminator wave" studies based on simple time coincidence. Both sunrise and sunset terminator waves are easily visualized in all of the monthly images. Our results confirm observations of [Forbes et al., GRL, 2008] of the wave structures existing on both sides of the terminator. The phase fronts of the sunset terminator wave are propagating downward indicating upward movement of the terminator-related disturbance and of the wave energy generated by it. The phase fronts of the sunrise terminator waves are propagating upward indicating downward movement of the terminator-related disturbance and of the wave energy generated by it. Spectral analysis of the local time sequences reveals characteristic peaks in the terminator-related wave activity corresponding to the periods 40-60 min and 2 hours. We also analyze statistics of their horizontal wavelengths.

Termination in cognitive-behavioral therapy with children, adolescents, and parents.

PubMed

Vidair, Hilary B; Feyijinmi, Grace O; Feindler, Eva L

2017-03-01

The process of terminating cognitive-behavioral therapy (CBT) with families has been largely neglected in the literature, with the limited research focused on premature termination. This article describes the natural termination process in CBT with children, adolescents, and their parents. Based on existing theories, we describe a cognitive-behavioral model for: (a) initiating and engaging in discussion of termination, (b) processing the termination of treatment and the therapeutic relationship, (c) key aspects of the termination process in the final session, and (d) the very end of the final session (saying goodbye). For each of the 4 components, we review relevant theories, provide clinical exchanges to demonstrate techniques, and provide related research support. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

Role of spiral wave pinning in inhomogeneous active media in the termination of atrial fibrillation by electrical cardioversion.

PubMed

Kuklik, Pawel; Wong, Christopher X; Brooks, Anthony G; Zebrowski, Jan Jacek; Sanders, Prashanthan

2010-03-01

Atrial fibrillation is the most common type of arrhythmia to affect humans. One of the treatment modalities for atrial fibrillation is an electrical cardioversion. Electrical cardioversion can result in one of three outcomes: an immediate termination of arrhythmic activity, a delayed termination or unsuccessful termination. The mechanism of delayed termination is unknown. Here we present a model of an atrial fibrillation as a coexistence of several spiral waves pinned to the inhomogeneities in active media. We show that in inhomogeneous system delayed termination can be explained as the unpinning of a spiral wave from inhomogeneities and its termination after collision with the edge of the system. Copyright (c) 2010 Elsevier Ltd. All rights reserved.

Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC.

PubMed

Tanco, Sebastian; Aviles, Francesc Xavier; Gevaert, Kris; Lorenzo, Julia; Van Damme, Petra

2017-01-01

We here present a detailed procedure for studying protein C-termini and their posttranslational modifications by C-terminal COFRADIC. In fact, this procedure can enrich for both C-terminal and N-terminal peptides through a combination of a strong cation exchange fractionation step at low pH, which removes the majority of nonterminal peptides in whole-proteome digests, while the actual COFRADIC step segregates C-terminal peptides from N-terminal peptides. When used in a differential mode, C-terminal COFRADIC allows for the identification of neo-C-termini generated by the action of proteases, which in turn leads to the identification of protease substrates. More specifically, this technology can be applied to determine the natural substrate repertoire of carboxypeptidases on a proteome-wide scale.

45 CFR 74.61 - Termination.

Code of Federal Regulations, 2010 CFR

2010-10-01

... AND SUBAWARDS TO INSTITUTIONS OF HIGHER EDUCATION, HOSPITALS, OTHER NONPROFIT ORGANIZATIONS, AND COMMERCIAL ORGANIZATIONS Post-Award Requirements Termination and Enforcement § 74.61 Termination. (a) Awards... two parties shall agree upon the termination conditions, including the effective date and, in the case...

29 CFR 95.61 - Termination.

Code of Federal Regulations, 2011 CFR

2011-07-01

...-PROFIT ORGANIZATIONS, AND WITH COMMERCIAL ORGANIZATIONS, FOREIGN GOVERNMENTS, ORGANIZATIONS UNDER THE JURISDICTION OF FOREIGN GOVERNMENTS, AND INTERNATIONAL ORGANIZATIONS Post-Award Requirements Termination and... effective date and, in the case of partial termination, the portion to be terminated. (3) By the recipient...

29 CFR 95.61 - Termination.

Code of Federal Regulations, 2010 CFR

2010-07-01

...-PROFIT ORGANIZATIONS, AND WITH COMMERCIAL ORGANIZATIONS, FOREIGN GOVERNMENTS, ORGANIZATIONS UNDER THE JURISDICTION OF FOREIGN GOVERNMENTS, AND INTERNATIONAL ORGANIZATIONS Post-Award Requirements Termination and... effective date and, in the case of partial termination, the portion to be terminated. (3) By the recipient...

48 CFR 49.304 - Procedure for partial termination.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Procedure for partial termination. 49.304 Section 49.304 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... Terminated for Convenience 49.304 Procedure for partial termination. ...

Network Terminations: A Compilation of Possible Answers.

ERIC Educational Resources Information Center

Wilson, John S.

An examination of 20 library network terminations reveals five major reasons for termination: lack of adequate funding, absorption by larger networks, loosely structured governance, partial termination of services, and networks programmed for short durations. Two tables present survey data. (RAA)

Nitrogen termination of single crystal (100) diamond surface by radio frequency N{sub 2} plasma process: An in-situ x-ray photoemission spectroscopy and secondary electron emission studies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chandran, Maneesh, E-mail: maneesh@tx.technion.ac.il, E-mail: choffman@tx.technion.ac.il; Shasha, Michal; Michaelson, Shaul

2015-09-14

In this letter, we report the electronic and chemical properties of nitrogen terminated (N-terminated) single crystal (100) diamond surface, which is a promising candidate for shallow NV{sup −} centers. N-termination is realized by an indirect RF nitrogen plasma process without inducing a large density of surface defects. Thermal stability and electronic property of N-terminated diamond surface are systematically investigated under well-controlled conditions by in-situ x-ray photoelectron spectroscopy and secondary electron emission. An increase in the low energy cut-off of the secondary electron energy distribution curve (EDC), with respect to a bare diamond surface, indicates a positive electron affinity of themore » N-terminated diamond. Exposure to atomic hydrogen results in reorganization of N-terminated diamond to H-terminated diamond, which exhibited a negative electron affinity surface. The change in intensity and spectral features of the secondary electron EDC of the N-terminated diamond is discussed.« less

Molecular coevolution of mammalian ribosomal gene terminator sequences and the transcription termination factor TTF-I.

PubMed Central

Evers, R; Grummt, I

1995-01-01

Both the DNA elements and the nuclear factors that direct termination of ribosomal gene transcription exhibit species-specific differences. Even between mammals--e.g., human and mouse--the termination signals are not identical and the respective transcription termination factors (TTFs) which bind to the terminator sequence are not fully interchangeable. To elucidate the molecular basis for this species-specificity, we have cloned TTF-I from human and mouse cells and compared their structural and functional properties. Recombinant TTF-I exhibits species-specific DNA binding and terminates transcription both in cell-free transcription assays and in transfection experiments. Chimeric constructs of mouse TTF-I and human TTF-I reveal that the major determinant for species-specific DNA binding resides within the C terminus of TTF-I. Replacing 31 C-terminal amino acids of mouse TTF-I with the homologous human sequences relaxes the DNA-binding specificity and, as a consequence, allows the chimeric factor to bind the human terminator sequence and to specifically stop rDNA transcription. Images Fig. 2 Fig. 3 Fig. 4 PMID:7597036

Concept of Operations for the Next Generation Air Transportation System. Version 3.2

DTIC Science & Technology

2011-01-01

Airside. Security Identification Display Area/Airport ( SIDA ) operations area, terminal perimeter, terminal airspace (security) • Landside. Terminal...Definition RTSS Remote Terminal Security Screening SAA Special Activity Airspace SIDA Security Identification Display Area SM Separation Management

Terminal location planning in intermodal transportation with Bayesian inference method.

DOT National Transportation Integrated Search

2014-01-01

In this project, we consider the planning of terminal locations for intermodal transportation systems. For a given number of potential terminals and coexisted multiple service pairs, we find the set of appropriate terminals and their locations that p...

7 CFR 1206.22 - Terminate.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Terminate. 1206.22 Section 1206.22 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... INFORMATION Mango Promotion, Research, and Information Order Definitions § 1206.22 Terminate. Terminate means...

7 CFR 953.66 - Termination.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Termination. 953.66 Section 953.66 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... STATES Order Regulating Handling Effective Time and Termination § 953.66 Termination. (a) The Secretary...

The application design of a mobile terminal: key technologies of mobile location-based service system

NASA Astrophysics Data System (ADS)

Tong, Xiaohui; Liu, Jingao; Wang, Zhenghao; Wang, Shuxian

2004-03-01

This paper presents several key technologies in the Mobile Location Based Service (MLBS) system of Shanghai. In the paper, the author presents a solution of handset mobile terminal, PDA+GPS+GSM/GPRS. The paper particularly introduces the hardware of the terminal and the acquirement of GPS information at the terminal. The paper also introduces how the terminal communicates with the special service center.

Battery system including batteries that have a plurality of positive terminals and a plurality of negative terminals

DOEpatents

Dougherty, Thomas J; Symanski, James S; Kuempers, Joerg A; Miles, Ronald C; Hansen, Scott A; Smith, Nels R; Taghikhani, Majid; Mrotek, Edward N; Andrew, Michael G

2014-01-21

A lithium battery for use in a vehicle includes a container, a plurality of positive terminals extending from a first end of the lithium battery, and a plurality of negative terminals extending from a second end of the lithium battery. The plurality of positive terminals are provided in a first configuration and the plurality of negative terminals are provided in a second configuration, the first configuration differing from the second configuration. A battery system for use in a vehicle may include a plurality of electrically connected lithium cells or batteries.

Enhancing thermoelectric properties through a three-terminal benzene molecule

NASA Astrophysics Data System (ADS)

Sartipi, Z.; Vahedi, J.

2018-05-01

The thermoelectric transport through a benzene molecule with three metallic terminals is discussed. Using general local and non-local transport coefficients, we investigated different conductance and thermopower coefficients within the linear response regime. Based on the Onsager coefficients which depend on the number of terminal efficiencies, efficiency at maximum power is also studied. In the three-terminal setup with tuning temperature differences, a great enhancement of the figure of merit is observed. Results also show that the third terminal model can be useful in improving the efficiency at maximum output power compared to the two-terminal model.

Low inductance connector assembly

DOEpatents

Holbrook, Meghan Ann; Carlson, Douglas S

2013-07-09

A busbar connector assembly for coupling first and second terminals on a two-terminal device to first and second contacts on a power module is provided. The first terminal resides proximate the first contact and the second terminal resides proximate the second contact. The assembly comprises a first bridge having a first end configured to be electrically coupled to the first terminal, and a second end configured to be electrically coupled to the second contact, and a second bridge substantially overlapping the first bridge and having a first end electrically coupled to the first contact, and a second end electrically coupled to the second terminal.

Spousal violence and pregnancy termination among married women in Nigeria.

PubMed

Bola, Solanke Lukman

2016-06-01

In Nigeria, the relationship between spousal violence and pregnancy termination had not been adequately explored. To assess the prevalence of spousal violence, and examine the relationship between spousal violence and pregnancy termination. Data on spousal violence among ever married women was extracted from the 2013 Nigeria Demographic and Health Survey. The outcome variable is pregnancy termination. The explanatory variables were the type of spousal violence experienced by the women in the last 12 months preceding the survey. Descriptive statistical analysis and binary logistic regression were applied using stata version 12. Results show that 13.8% of women had ever terminated pregnancy; 19.9% had ever experienced at least one type of spousal violence; and women who had ever terminated pregnancy had higher prevalence of all types of spousal violence. Women who had ever experienced spousal physical violence were 9% more likely to experience pregnancy termination (OR=1.09; CI: 1.03-2.86); and women who had ever experienced spousal emotional violence were 33% more likely to experience pregnancy termination (OR=1.33; CI: 0.97-1.95). Spousal violence is significantly related to pregnancy termination. Improving women's sexual and reproductive health in the country requires fresh initiatives that address spousal violence to further reduce women's exposure to pregnancy termination.

State Regulatory Enforcement and Nursing Home Termination from the Medicare and Medicaid Programs

PubMed Central

Li, Yue; Harrington, Charlene; Spector, William D; Mukamel, Dana B

2010-01-01

Objectives Nursing homes certified by the Medicare and/or Medicaid program are subject to federally mandated and state-enforced quality and safety standards. We examined the relationship between state quality enforcement and nursing home terminations from the two programs. Study Design Using data from a survey of state licensure and certification agencies and other secondary databases, we performed bivariate and multivariate analyses on the strength of state quality regulation in 2005, and nursing home voluntary terminations (decisions made by the facility) or involuntary terminations (imposed by the state) in 2006–2007. Principal Findings Involuntary terminations were rarely imposed by state regulators, while voluntary terminations were relatively more common (2.16 percent in 2006–2007) and varied considerably across states. After controlling for facility, market, and state covariates, nursing homes in states implementing stronger quality enforcement were more likely to voluntarily terminate from the Medicare and Medicaid programs (odds ratio=1.53, p=.018). Conclusions Although involuntary nursing home terminations occurred rarely in most states, nursing homes in states with stronger quality regulations tend to voluntarily exit the publicly financed market. Because of the consequences of voluntary terminations on patient care and access, state regulators need to consider the effects of increased enforcement on both enhanced quality and the costs of termination. PMID:20819106

Analyzing semi-competing risks data with missing cause of informative terminal event.

PubMed

Zhou, Renke; Zhu, Hong; Bondy, Melissa; Ning, Jing

2017-02-28

Cancer studies frequently yield multiple event times that correspond to landmarks in disease progression, including non-terminal events (i.e., cancer recurrence) and an informative terminal event (i.e., cancer-related death). Hence, we often observe semi-competing risks data. Work on such data has focused on scenarios in which the cause of the terminal event is known. However, in some circumstances, the information on cause for patients who experience the terminal event is missing; consequently, we are not able to differentiate an informative terminal event from a non-informative terminal event. In this article, we propose a method to handle missing data regarding the cause of an informative terminal event when analyzing the semi-competing risks data. We first consider the nonparametric estimation of the survival function for the terminal event time given missing cause-of-failure data via the expectation-maximization algorithm. We then develop an estimation method for semi-competing risks data with missing cause of the terminal event, under a pre-specified semiparametric copula model. We conduct simulation studies to investigate the performance of the proposed method. We illustrate our methodology using data from a study of early-stage breast cancer. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

RNA polymerase III mutants in TFIIFα-like C37 that cause terminator readthrough with no decrease in transcription output

PubMed Central

Rijal, Keshab; Maraia, Richard J.

2013-01-01

How eukaryotic RNA polymerases switch from elongation to termination is unknown. Pol III subunits Rpc53 and Rpc37 (C53/37) form a heterodimer homologous to TFIIFβ/α. C53/37 promotes efficient termination and together with C11 also mediates pol III recycling in vitro. We previously developed Schizosaccharomyces pombe strains that report on two pol III termination activities: RNA oligo(U) 3′-end cleavage, and terminator readthrough. We randomly mutagenized C53 and C37 and isolated many C37 mutants with terminator readthrough but no comparable C53 mutants. The majority of C37 mutants have strong phenotypes with up to 40% readthrough and map to a C-terminal tract previously localized near Rpc2p in the pol III active center while a minority represent a distinct class with weaker phenotype, less readthrough and 3′-oligo(U) lengthening. Nascent pre-tRNAs released from a terminator by C37 mutants have shorter 3′-oligo(U) tracts than in cleavage-deficient C11 double mutants indicating RNA 3′-end cleavage during termination. We asked whether termination deficiency affects transcription output in the mutants in vivo both by monitoring intron-containing nascent transcript levels and 14C-uridine incorporation. Surprisingly, multiple termination mutants have no decrease in transcript output relative to controls. These data are discussed in context of current models of pol III transcription. PMID:23093604

Synthetic Minor NSR Permit: Van Hook Crude Terminal, LLC - Van Hook Crude Terminal/Rail Loading Facility

EPA Pesticide Factsheets

This page contains the current effective synthetic minor NSR permit for the Van Hook Crude Terminal, LLC, Van Hook Crude Terminal/Rail Loading Facility, located on the Fort Berthold Indian Reservation in Mountrail County, ND.

48 CFR 249.7000 - Terminated contracts with Canadian Commercial Corporation.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Canadian Commercial Corporation. 249.7000 Section 249.7000 Federal Acquisition Regulations System DEFENSE... Termination Requirements 249.7000 Terminated contracts with Canadian Commercial Corporation. (a) Terminate contracts with the Canadian Commercial Corporation in accordance with— (1) The Letter of Agreement (LOA...

48 CFR 249.7000 - Terminated contracts with Canadian Commercial Corporation.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Canadian Commercial Corporation. 249.7000 Section 249.7000 Federal Acquisition Regulations System DEFENSE... Termination Requirements 249.7000 Terminated contracts with Canadian Commercial Corporation. (a) Terminate contracts with the Canadian Commercial Corporation in accordance with— (1) The Letter of Agreement (LOA...

48 CFR 249.7000 - Terminated contracts with Canadian Commercial Corporation.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Canadian Commercial Corporation. 249.7000 Section 249.7000 Federal Acquisition Regulations System DEFENSE... Termination Requirements 249.7000 Terminated contracts with Canadian Commercial Corporation. (a) Terminate contracts with the Canadian Commercial Corporation in accordance with— (1) The Letter of Agreement (LOA...

48 CFR 8.406-5 - Termination for the Government's convenience.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Government's convenience. 8.406-5 Section 8.406-5 Federal Acquisition Regulations System FEDERAL ACQUISITION... Termination for the Government's convenience. (a) An ordering activity contracting officer may terminate individual orders for the Government's convenience. Terminations for the Government's convenience shall...

22 CFR 518.61 - Termination.

Code of Federal Regulations, 2010 CFR

2010-04-01

... INSTITUTIONS OF HIGHER EDUCATION, HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements... conditions, including the effective date and, in the case of partial termination, the portion to be... forth the reasons for such termination, the effective date, and, in the case of partial termination, the...

36 CFR 1210.61 - Termination.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Termination and Enforcement § 1210.61... agree upon the termination conditions, including the effective date and, in the case of partial... setting forth the reasons for such termination, the effective date, and, in the case of partial...

28 CFR 70.61 - Termination.

Code of Federal Regulations, 2011 CFR

2011-07-01

... (INCLUDING SUBAWARDS) WITH INSTITUTIONS OF HIGHER EDUCATION, HOSPITALS AND OTHER NON-PROFIT ORGANIZATIONS... conditions, including the effective date and, in the case of partial termination, the portion to be... reasons for such termination, the effective date, and, in the case of partial termination, the portion to...

22 CFR 518.61 - Termination.

Code of Federal Regulations, 2011 CFR

2011-04-01

... INSTITUTIONS OF HIGHER EDUCATION, HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements... conditions, including the effective date and, in the case of partial termination, the portion to be... forth the reasons for such termination, the effective date, and, in the case of partial termination, the...

48 CFR 49.502 - Termination for convenience of the Government.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Termination for convenience of the Government. 49.502 Section 49.502 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT TERMINATION OF CONTRACTS Contract Termination Clauses 49.502...

75 FR 67749 - Notice of Agreements Filed

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-03

... Association. Parties: Ports Baltimore, Inc.; Maryland International Terminals, Inc.; Mid-Atlantic Terminal LLC; Ceres Marine terminals, Inc.; Tartan Terminals, Inc. and Ports America Chesapeake, Inc. Filing Party: Jo.... Synopsis: The amendment increases the amount of space CMA CGM is allotted from Pacific Northwest ports...

75 FR 22675 - Notice of Passenger Facility Charge (PFC) Approvals and Disapprovals

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-29

... expansion. Security screening expansion. Terminal restroom design. Terminal restroom remodel. Terminal... remodeling. Pavement management system. Master plan, phase 1. Decision Date: September 23, 2009. FOR FURTHER... modifications, passenger terminal. Rehabilitation of taxilane K-1. Design and implement noise mitigation...

48 CFR 49.502 - Termination for convenience of the Government.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Termination for convenience of the Government. 49.502 Section 49.502 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT TERMINATION OF CONTRACTS Contract Termination Clauses 49.502...

48 CFR 49.502 - Termination for convenience of the Government.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Termination for convenience of the Government. 49.502 Section 49.502 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT TERMINATION OF CONTRACTS Contract Termination Clauses 49.502...

48 CFR 49.502 - Termination for convenience of the Government.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Termination for convenience of the Government. 49.502 Section 49.502 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT TERMINATION OF CONTRACTS Contract Termination Clauses 49.502...

48 CFR 49.102 - Notice of termination.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Notice of termination. 49.102 Section 49.102 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT TERMINATION OF CONTRACTS General Principles 49.102 Notice of termination. (a) General. The...

48 CFR 49.115 - Settlement of terminated incentive contracts.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Settlement of terminated incentive contracts. 49.115 Section 49.115 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT TERMINATION OF CONTRACTS General Principles 49.115 Settlement of terminated...

Analyses of interactions between the marine terminal and highway operations : final report.

DOT National Transportation Integrated Search

2014-04-01

Changes in vessel sizes arriving at United States ports may influence the operating regimes and schedules at the port terminals However, the impacts to highway and rail operations outside of the port terminals because of changing port terminal operat...

47 CFR 51.715 - Interim transport and termination pricing.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 3 2010-10-01 2010-10-01 false Interim transport and termination pricing. 51... SERVICES (CONTINUED) INTERCONNECTION Reciprocal Compensation for Transport and Termination of Telecommunications Traffic § 51.715 Interim transport and termination pricing. (a) Upon request from a...

47 CFR 51.715 - Interim transport and termination pricing.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 3 2011-10-01 2011-10-01 false Interim transport and termination pricing. 51... SERVICES (CONTINUED) INTERCONNECTION Reciprocal Compensation for Transport and Termination of Telecommunications Traffic § 51.715 Interim transport and termination pricing. (a) Upon request from a...

7 CFR 946.64 - Proceedings after termination.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Proceedings after termination. 946.64 Section 946.64 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... WASHINGTON Order Regulating Handling Effective Time and Termination § 946.64 Proceedings after termination...

49 CFR 374.309 - Terminal facilities.

Code of Federal Regulations, 2011 CFR

2011-10-01

...) Passenger security. All terminals and stations must provide adequate security for passengers and their attendants and be regularly patrolled. (b) Outside facilities. At terminals and stations that are closed when... accommodations, and telephone numbers for local taxi service and police. (c) Maintenance. Terminals shall be...

13 CFR 304.3 - District modification and termination.

Code of Federal Regulations, 2010 CFR

2010-01-01

... termination. 304.3 Section 304.3 Business Credit and Assistance ECONOMIC DEVELOPMENT ADMINISTRATION, DEPARTMENT OF COMMERCE ECONOMIC DEVELOPMENT DISTRICTS § 304.3 District modification and termination. (a... for economic development. (b) Termination. EDA may, upon sixty (60) days prior written notice to the...

The transcriptional terminator sequences downstream of the covR gene terminate covR/S operon transcription to generate covR monocistronic transcripts in Streptococcus pyogenes.

PubMed

Chiang-Ni, Chuan; Tsou, Chih-Cheng; Lin, Yee-Shin; Chuang, Woei-Jer; Lin, Ming-T; Liu, Ching-Chuan; Wu, Jiunn-Jong

2008-12-31

CovR/S is an important two component regulatory system, which regulates about 15% of the gene expression in Streptococcus pyogenes. The covR/S locus was identified as an operon generating an RNA transcript around 2.5-kb in size. In this study, we found the covR/S operon produced three RNA transcripts (around 2.5-, 1.0-, and 0.8-kb in size). Using RNA transcriptional terminator sequence prediction and transcriptional terminator analysis, we identified two atypical rho-independent terminator sequences downstream of the covR gene and showed these terminator sequences terminate RNA transcription efficiently. These results indicate that covR/S operon generates covR/S transcript and monocistronic covR transcripts.

Na+ current in presynaptic terminals of the crayfish opener cannot initiate action potentials.

PubMed

Lin, Jen-Wei

2016-01-01

Action potential (AP) propagation in presynaptic axons of the crayfish opener neuromuscular junction (NMJ) was investigated by simultaneously recording from a terminal varicosity and a proximal branch. Although orthodromically conducting APs could be recorded in terminals with amplitudes up to 70 mV, depolarizing steps in terminals to -20 mV or higher failed to fire APs. Patch-clamp recordings did detect Na(+) current (INa) in most terminals. The INa exhibited a high threshold and fast activation rate. Local perfusion of Na(+)-free saline showed that terminal INa contributed to AP waveform by slightly accelerating the rising phase and increasing the peak amplitude. These findings suggest that terminal INa functions to "touch up" but not to generate APs. Copyright © 2016 the American Physiological Society.

Reading the Road Signs: The Utility of the MMPI-2 Restructured Form Validity Scales in Prediction of Premature Termination.

PubMed

Anestis, Joye C; Finn, Jacob A; Gottfried, Emily; Arbisi, Paul A; Joiner, Thomas E

2015-06-01

This study examined the utility of the Minnesota Multiphasic Personality Inventory-2 Restructured Form (MMPI-2-RF) Validity Scales in prediction of premature termination in a sample of 511 individuals seeking services from a university-based psychology clinic. Higher scores on True Response Inconsistency-Revised and Infrequent Psychopathology Responses increased the risk of premature termination, whereas higher scores on Adjustment Validity lowered the risk of premature termination. Additionally, when compared with individuals who did not prematurely terminate, individuals who prematurely terminated treatment had lower Global Assessment of Functioning scores at both intake and termination and made fewer improvements. Implications of these findings for the use of the MMPI-2-RF Validity Scales in promoting treatment compliance are discussed. © The Author(s) 2014.

A model for regulation of mammalian ribosomal DNA transcription. Co-ordination of initiation and termination.

PubMed Central

Nashimoto, M; Mishima, Y

1988-01-01

Based on recent experimental data about transcription initiation and termination, a model for regulation of mammalian ribosomal DNA transcription is developed using a simple kinetic scheme. In this model, the existence of the transition pathway from the terminator to the promoter increases the rate of ribosomal RNA precursor synthesis. In addition to this 'non-transcribed spacer' traverse of RNA polymerase I, the co-ordination of initiation and termination allows a rapid on/off switch transition from the minimum to the maximum rate of ribosomal RNA precursor synthesis. Furthermore, taking account of the participation of two factors in the termination event, we propose a plausible molecular mechanism for the co-ordination of initiation and termination. This co-ordination is emphasized by repetition of the terminator unit. PMID:3223915

ACTS propagation experiment discussion: Ka-band propagation measurements using the ACTS propagation terminal and the CSU-CHILL and Space Communications Technology Center Florida propagation program

NASA Technical Reports Server (NTRS)

Bringi, V. N.; Chandrasekar, V.; Mueller, Eugene A.; Turk, Joseph; Beaver, John; Helmken, Henry F.; Henning, Rudy

1993-01-01

Papers on Ka-band propagation measurements using the ACTS propagation terminal and the Colorado State University CHILL multiparameter radar and on Space Communications Technology Center Florida Propagation Program are discussed. Topics covered include: microwave radiative transfer and propagation models; NASA propagation terminal status; ACTS channel characteristics; FAU receive only terminal; FAU terminal status; and propagation testbed.

Contributions of the N- and C-terminal helical segments to the lipid-free structure and lipid interaction of apolipoprotein A-I.

PubMed

Tanaka, Masafumi; Dhanasekaran, Padmaja; Nguyen, David; Ohta, Shinya; Lund-Katz, Sissel; Phillips, Michael C; Saito, Hiroyuki

2006-08-29

The tertiary structure of lipid-free apolipoprotein (apo) A-I in the monomeric state comprises two domains: a N-terminal alpha-helix bundle and a less organized C-terminal domain. This study examined how the N- and C-terminal segments of apoA-I (residues 1-43 and 223-243), which contain the most hydrophobic regions in the molecule and are located in opposite structural domains, contribute to the lipid-free conformation and lipid interaction. Measurements of circular dichroism in conjunction with tryptophan and 8-anilino-1-naphthalenesulfonic acid fluorescence data demonstrated that single (L230P) or triple (L230P/L233P/Y236P) proline insertions into the C-terminal alpha helix disrupted the organization of the C-terminal domain without affecting the stability of the N-terminal helix bundle. In contrast, proline insertion into the N terminus (Y18P) disrupted the bundle structure in the N-terminal domain, indicating that the alpha-helical segment in this region is part of the helix bundle. Calorimetric and gel-filtration measurements showed that disruption of the C-terminal alpha helix significantly reduced the enthalpy and free energy of binding of apoA-I to lipids, whereas disruption of the N-terminal alpha helix had only a small effect on lipid binding. Significantly, the presence of the Y18P mutation offset the negative effects of disruption/removal of the C-terminal helical domain on lipid binding, suggesting that the alpha helix around Y18 concealed a potential lipid-binding region in the N-terminal domain, which was exposed by the disruption of the helix-bundle structure. When these results are taken together, they indicate that the alpha-helical segment in the N terminus of apoA-I modulates the lipid-free structure and lipid interaction in concert with the C-terminal domain.

Use of High Capacity Terminators in Saccharomyces cerevisiae to Increase mRNA half-life and Improve Gene Expression Control for Metabolic Engineering Applications

PubMed Central

Curran, Kathleen A.; Karim, Ashty S.; Gupta, Akash; Alper, Hal S.

2013-01-01

Control of gene and protein expression of both endogenous and heterologous genes is a key component of metabolic engineering. While a large amount of work has been published characterizing promoters for this purpose, less effort has been exerted to elucidate the role of terminators in yeast. In this study, we characterize over 30 terminators for use in metabolic engineering applications in Saccharomyces cerevisiae and determine mRNA half-life changes to be the major cause of the varied protein and transcript expression level. We demonstrate that the difference in transcript level can be over 6.5-fold even for high strength promoters. The influence of terminator selection is magnified when coupled with a low-expression promoter, with a maximum difference in protein expression of 11-fold between a high-capacity terminator and the parent plasmid terminator and over 35-fold difference when compared with a no-terminator baseline. This is the first time that terminators have been investigated in the context of multiple promoters spanning orders of magnitude in activity. Finally, we demonstrate the utility of terminator selection for metabolic engineering by using a mutant xylose isomerase gene as a proof-of-concept. Through pairing a high-capacity terminator with a low-expression promoter, we were able to achieve the same phenotypic result as with a promoter considerably higher in strength. Moreover, we can further boost the phenotype of the high-strength promoter by pairing it with a high-capacity terminator. This work highlights how terminator elements can be used to control metabolic pathways in the same way that promoters are traditionally used in yeast. Together, this work demonstrates that terminators will be an important part of heterologous gene expression and metabolic engineering for yeast in the future. PMID:23856240

Terminated Trials in the ClinicalTrials.gov Results Database: Evaluation of Availability of Primary Outcome Data and Reasons for Termination

PubMed Central

Williams, Rebecca J.; Tse, Tony; DiPiazza, Katelyn; Zarin, Deborah A.

2015-01-01

Background Clinical trials that end prematurely (or “terminate”) raise financial, ethical, and scientific concerns. The extent to which the results of such trials are disseminated and the reasons for termination have not been well characterized. Methods and Findings A cross-sectional, descriptive study of terminated clinical trials posted on the ClinicalTrials.gov results database as of February 2013 was conducted. The main outcomes were to characterize the availability of primary outcome data on ClinicalTrials.gov and in the published literature and to identify the reasons for trial termination. Approximately 12% of trials with results posted on the ClinicalTrials.gov results database (905/7,646) were terminated. Most trials were terminated for reasons other than accumulated data from the trial (68%; 619/905), with an insufficient rate of accrual being the lead reason for termination among these trials (57%; 350/619). Of the remaining trials, 21% (193/905) were terminated based on data from the trial (findings of efficacy or toxicity) and 10% (93/905) did not specify a reason. Overall, data for a primary outcome measure were available on ClinicalTrials.gov and in the published literature for 72% (648/905) and 22% (198/905) of trials, respectively. Primary outcome data were reported on the ClinicalTrials.gov results database and in the published literature more frequently (91% and 46%, respectively) when the decision to terminate was based on data from the trial. Conclusions Trials terminate for a variety of reasons, not all of which reflect failures in the process or an inability to achieve the intended goals. Primary outcome data were reported most often when termination was based on data from the trial. Further research is needed to identify best practices for disseminating the experience and data resulting from terminated trials in order to help ensure maximal societal benefit from the investments of trial participants and others involved with the study. PMID:26011295

Developmental up-regulation of vesicular glutamate transporter-1 promotes neocortical presynaptic terminal development.

PubMed

Berry, Corbett T; Sceniak, Michael P; Zhou, Louie; Sabo, Shasta L

2012-01-01

Presynaptic terminal formation is a complex process that requires assembly of proteins responsible for synaptic transmission at sites of axo-dendritic contact. Accumulation of presynaptic proteins at developing terminals is facilitated by glutamate receptor activation. Glutamate is loaded into synaptic vesicles for release via the vesicular glutamate transporters VGLUT1 and VGLUT2. During postnatal development there is a switch from predominantly VGLUT2 expression to high VGLUT1 and low VGLUT2, raising the question of whether the developmental increase in VGLUT1 is important for presynaptic development. Here, we addressed this question using confocal microscopy and quantitative immunocytochemistry in primary cultures of rat neocortical neurons. First, in order to understand the extent to which the developmental switch from VGLUT2 to VGLUT1 occurs through an increase in VGLUT1 at individual presynaptic terminals or through addition of VGLUT1-positive presynaptic terminals, we examined the spatio-temporal dynamics of VGLUT1 and VGLUT2 expression. Between 5 and 12 days in culture, the percentage of presynaptic terminals that expressed VGLUT1 increased during synapse formation, as did expression of VGLUT1 at individual terminals. A subset of VGLUT1-positive terminals also expressed VGLUT2, which decreased at these terminals. At individual terminals, the increase in VGLUT1 correlated with greater accumulation of other synaptic vesicle proteins, such as synapsin and synaptophysin. When the developmental increase in VGLUT1 was prevented using VGLUT1-shRNA, the density of presynaptic terminals and accumulation of synapsin and synaptophysin at terminals were decreased. Since VGLUT1 knock-down was limited to a small number of neurons, the observed effects were cell-autonomous and independent of changes in overall network activity. These results demonstrate that up-regulation of VGLUT1 is important for development of presynaptic terminals in the cortex.

Developmental Up-Regulation of Vesicular Glutamate Transporter-1 Promotes Neocortical Presynaptic Terminal Development

PubMed Central

Berry, Corbett T.; Sceniak, Michael P.; Zhou, Louie; Sabo, Shasta L.

2012-01-01

Presynaptic terminal formation is a complex process that requires assembly of proteins responsible for synaptic transmission at sites of axo-dendritic contact. Accumulation of presynaptic proteins at developing terminals is facilitated by glutamate receptor activation. Glutamate is loaded into synaptic vesicles for release via the vesicular glutamate transporters VGLUT1 and VGLUT2. During postnatal development there is a switch from predominantly VGLUT2 expression to high VGLUT1 and low VGLUT2, raising the question of whether the developmental increase in VGLUT1 is important for presynaptic development. Here, we addressed this question using confocal microscopy and quantitative immunocytochemistry in primary cultures of rat neocortical neurons. First, in order to understand the extent to which the developmental switch from VGLUT2 to VGLUT1 occurs through an increase in VGLUT1 at individual presynaptic terminals or through addition of VGLUT1-positive presynaptic terminals, we examined the spatio-temporal dynamics of VGLUT1 and VGLUT2 expression. Between 5 and 12 days in culture, the percentage of presynaptic terminals that expressed VGLUT1 increased during synapse formation, as did expression of VGLUT1 at individual terminals. A subset of VGLUT1-positive terminals also expressed VGLUT2, which decreased at these terminals. At individual terminals, the increase in VGLUT1 correlated with greater accumulation of other synaptic vesicle proteins, such as synapsin and synaptophysin. When the developmental increase in VGLUT1 was prevented using VGLUT1-shRNA, the density of presynaptic terminals and accumulation of synapsin and synaptophysin at terminals were decreased. Since VGLUT1 knock-down was limited to a small number of neurons, the observed effects were cell-autonomous and independent of changes in overall network activity. These results demonstrate that up-regulation of VGLUT1 is important for development of presynaptic terminals in the cortex. PMID:23226425

77 FR 71178 - Termination of Department of Defense Federal Advisory Committees

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-29

... DEPARTMENT OF DEFENSE Office of the Secretary Termination of Department of Defense Federal Advisory Committees AGENCY: Department of Defense. ACTION: Termination of Federal Advisory Committee..., the Department of Defense gives notice that it is terminating the Chief of Naval Operations Executive...

40 CFR 721.6485 - Hydroxy terminated polyester.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Hydroxy terminated polyester. 721.6485... Substances § 721.6485 Hydroxy terminated polyester. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically as a hydroxy terminated polyester...

40 CFR 721.6485 - Hydroxy terminated polyester.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Hydroxy terminated polyester. 721.6485... Substances § 721.6485 Hydroxy terminated polyester. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically as a hydroxy terminated polyester...

48 CFR 49.601 - Notice of termination for convenience.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Notice of termination for convenience. 49.601 Section 49.601 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... termination for convenience. (See 49.402-3(g) for notice of termination for default.) ...

22 CFR 145.61 - Termination.

Code of Federal Regulations, 2010 CFR

2010-04-01

..., HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Termination and Enforcement § 145.61... parties shall agree upon the termination conditions, including the effective date and, in the case of... written notification setting forth the reasons for such termination, the effective date, and, in the case...

22 CFR 145.61 - Termination.

Code of Federal Regulations, 2011 CFR

2011-04-01

..., HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Termination and Enforcement § 145.61... parties shall agree upon the termination conditions, including the effective date and, in the case of... written notification setting forth the reasons for such termination, the effective date, and, in the case...

77 FR 18849 - Notice of Proposed Information Collection: Comment Request: Insurance Termination Request for...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-28

... Information Collection: Comment Request: Insurance Termination Request for Multifamily Mortgage AGENCY: Office... also lists the following information: Title of Proposal: Insurance Termination Request for Multifamily... mortgagee mutually agree to terminate the HUD multifamily mortgage insurance. Agency form numbers, if...

5 CFR 870.907 - Termination and conversion.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 5 Administrative Personnel 2 2010-01-01 2010-01-01 false Termination and conversion. 870.907....907 Termination and conversion. (a) Assigned insurance terminates under the conditions stated in... time the conversion policy is issued. (5) The employing office must notify each assignee of the...

5 CFR 870.907 - Termination and conversion.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 5 Administrative Personnel 2 2011-01-01 2011-01-01 false Termination and conversion. 870.907....907 Termination and conversion. (a) Assigned insurance terminates under the conditions stated in... time the conversion policy is issued. (5) The employing office must notify each assignee of the...

47 CFR 2.1051 - Measurements required: Spurious emissions at antenna terminals.

Code of Federal Regulations, 2014 CFR

2014-10-01

... antenna terminals. 2.1051 Section 2.1051 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL... Procedures Certification § 2.1051 Measurements required: Spurious emissions at antenna terminals. The radio... checked at the equipment output terminals when properly loaded with a suitable artificial antenna. Curves...

47 CFR 2.1051 - Measurements required: Spurious emissions at antenna terminals.

Code of Federal Regulations, 2010 CFR

2010-10-01

... antenna terminals. 2.1051 Section 2.1051 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL... Procedures Certification § 2.1051 Measurements required: Spurious emissions at antenna terminals. The radio... checked at the equipment output terminals when properly loaded with a suitable artificial antenna. Curves...

47 CFR 2.1051 - Measurements required: Spurious emissions at antenna terminals.

Code of Federal Regulations, 2013 CFR

2013-10-01

... antenna terminals. 2.1051 Section 2.1051 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL... Procedures Certification § 2.1051 Measurements required: Spurious emissions at antenna terminals. The radio... checked at the equipment output terminals when properly loaded with a suitable artificial antenna. Curves...

47 CFR 2.1051 - Measurements required: Spurious emissions at antenna terminals.

Code of Federal Regulations, 2011 CFR

2011-10-01

... antenna terminals. 2.1051 Section 2.1051 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL... Procedures Certification § 2.1051 Measurements required: Spurious emissions at antenna terminals. The radio... checked at the equipment output terminals when properly loaded with a suitable artificial antenna. Curves...

47 CFR 2.1051 - Measurements required: Spurious emissions at antenna terminals.

Code of Federal Regulations, 2012 CFR

2012-10-01

... antenna terminals. 2.1051 Section 2.1051 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL... Procedures Certification § 2.1051 Measurements required: Spurious emissions at antenna terminals. The radio... checked at the equipment output terminals when properly loaded with a suitable artificial antenna. Curves...

48 CFR 749.111-70 - Termination settlement review boards.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Termination settlement review boards. 749.111-70 Section 749.111-70 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT CONTRACT MANAGEMENT TERMINATION OF CONTRACTS General Principles 749.111-70 Termination...

48 CFR 49.105-1 - Termination status reports.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Termination status reports. 49.105-1 Section 49.105-1 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT TERMINATION OF CONTRACTS General Principles 49.105-1 Termination status reports. When...

28 CFR 2.96 - Order of early termination.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Order of early termination. 2.96 Section 2.96 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND RECOMMITMENT OF....96 Order of early termination. When the Commission orders early termination from supervision, the...

28 CFR 2.96 - Order of early termination.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 28 Judicial Administration 1 2011-07-01 2011-07-01 false Order of early termination. 2.96 Section 2.96 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND RECOMMITMENT OF....96 Order of early termination. When the Commission orders early termination from supervision, the...

10 CFR 20.1404 - Alternate criteria for license termination.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 1 2013-01-01 2013-01-01 false Alternate criteria for license termination. 20.1404 Section 20.1404 Energy NUCLEAR REGULATORY COMMISSION STANDARDS FOR PROTECTION AGAINST RADIATION Radiological Criteria for License Termination § 20.1404 Alternate criteria for license termination. (a) The...

10 CFR 20.1404 - Alternate criteria for license termination.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 1 2011-01-01 2011-01-01 false Alternate criteria for license termination. 20.1404 Section 20.1404 Energy NUCLEAR REGULATORY COMMISSION STANDARDS FOR PROTECTION AGAINST RADIATION Radiological Criteria for License Termination § 20.1404 Alternate criteria for license termination. (a) The...

10 CFR 20.1404 - Alternate criteria for license termination.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 1 2014-01-01 2014-01-01 false Alternate criteria for license termination. 20.1404 Section 20.1404 Energy NUCLEAR REGULATORY COMMISSION STANDARDS FOR PROTECTION AGAINST RADIATION Radiological Criteria for License Termination § 20.1404 Alternate criteria for license termination. (a) The...

10 CFR 20.1404 - Alternate criteria for license termination.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 1 2010-01-01 2010-01-01 false Alternate criteria for license termination. 20.1404 Section 20.1404 Energy NUCLEAR REGULATORY COMMISSION STANDARDS FOR PROTECTION AGAINST RADIATION Radiological Criteria for License Termination § 20.1404 Alternate criteria for license termination. (a) The...

33 CFR 159.79 - Terminals.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Terminals. 159.79 Section 159.79 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) POLLUTION MARINE SANITATION DEVICES Design, Construction, and Testing § 159.79 Terminals. Terminals must be solderless lugs...

47 CFR 51.709 - Rate structure for transport and termination.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 3 2010-10-01 2010-10-01 false Rate structure for transport and termination... SERVICES (CONTINUED) INTERCONNECTION Reciprocal Compensation for Transport and Termination of Telecommunications Traffic § 51.709 Rate structure for transport and termination. (a) In state proceedings, a state...

47 CFR 51.709 - Rate structure for transport and termination.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 3 2011-10-01 2011-10-01 false Rate structure for transport and termination... SERVICES (CONTINUED) INTERCONNECTION Reciprocal Compensation for Transport and Termination of Telecommunications Traffic § 51.709 Rate structure for transport and termination. (a) In state proceedings, a state...

22 CFR 42.83 - Termination of registration.

Code of Federal Regulations, 2012 CFR

2012-04-01

....83 Foreign Relations DEPARTMENT OF STATE VISAS VISAS: DOCUMENTATION OF IMMIGRANTS UNDER THE... Termination of registration. (a) Termination following failure of applicant to apply for visa. In accordance with INA 203(g), an alien's registration for an immigrant visa shall be terminated if, within one year...

22 CFR 42.83 - Termination of registration.

Code of Federal Regulations, 2011 CFR

2011-04-01

....83 Foreign Relations DEPARTMENT OF STATE VISAS VISAS: DOCUMENTATION OF IMMIGRANTS UNDER THE... Termination of registration. (a) Termination following failure of applicant to apply for visa. In accordance with INA 203(g), an alien's registration for an immigrant visa shall be terminated if, within one year...

22 CFR 42.83 - Termination of registration.

Code of Federal Regulations, 2010 CFR

2010-04-01

....83 Foreign Relations DEPARTMENT OF STATE VISAS VISAS: DOCUMENTATION OF IMMIGRANTS UNDER THE... Termination of registration. (a) Termination following failure of applicant to apply for visa. In accordance with INA 203(g), an alien's registration for an immigrant visa shall be terminated if, within one year...

22 CFR 42.83 - Termination of registration.

Code of Federal Regulations, 2014 CFR

2014-04-01

....83 Foreign Relations DEPARTMENT OF STATE VISAS VISAS: DOCUMENTATION OF IMMIGRANTS UNDER THE... Termination of registration. (a) Termination following failure of applicant to apply for visa. In accordance with INA 203(g), an alien's registration for an immigrant visa shall be terminated if, within one year...

22 CFR 42.83 - Termination of registration.

Code of Federal Regulations, 2013 CFR

2013-04-01

....83 Foreign Relations DEPARTMENT OF STATE VISAS VISAS: DOCUMENTATION OF IMMIGRANTS UNDER THE... Termination of registration. (a) Termination following failure of applicant to apply for visa. In accordance with INA 203(g), an alien's registration for an immigrant visa shall be terminated if, within one year...

42 CFR 66.109 - Termination.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 42 Public Health 1 2013-10-01 2013-10-01 false Termination. 66.109 Section 66.109 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING NATIONAL RESEARCH SERVICE AWARDS Direct Awards § 66.109 Termination. (a) The Secretary may terminate an...

42 CFR 66.109 - Termination.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 42 Public Health 1 2014-10-01 2014-10-01 false Termination. 66.109 Section 66.109 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING NATIONAL RESEARCH SERVICE AWARDS Direct Awards § 66.109 Termination. (a) The Secretary may terminate an...

42 CFR 66.109 - Termination.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 1 2010-10-01 2010-10-01 false Termination. 66.109 Section 66.109 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING NATIONAL RESEARCH SERVICE AWARDS Direct Awards § 66.109 Termination. (a) The Secretary may terminate an...

42 CFR 66.109 - Termination.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 1 2011-10-01 2011-10-01 false Termination. 66.109 Section 66.109 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING NATIONAL RESEARCH SERVICE AWARDS Direct Awards § 66.109 Termination. (a) The Secretary may terminate an...

42 CFR 66.109 - Termination.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 42 Public Health 1 2012-10-01 2012-10-01 false Termination. 66.109 Section 66.109 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING NATIONAL RESEARCH SERVICE AWARDS Direct Awards § 66.109 Termination. (a) The Secretary may terminate an...

20 CFR 416.1335 - Termination due to continuous suspension.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false Termination due to continuous suspension. 416.1335 Section 416.1335 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Suspensions and Terminations § 416.1335 Termination due to...

Redundancy of primary RNA-binding functions of the bacterial transcription terminator Rho

PubMed Central

Shashni, Rajesh; Qayyum, M. Zuhaib; Vishalini, V.; Dey, Debashish; Sen, Ranjan

2014-01-01

The bacterial transcription terminator, Rho, terminates transcription at half of the operons. According to the classical model derived from in vitro assays on a few terminators, Rho is recruited to the transcription elongation complex (EC) by recognizing specific sites (rut) on the nascent RNA. Here, we explored the mode of in vivo recruitment process of Rho. We show that sequence specific recognition of the rut site, in majority of the Rho-dependent terminators, can be compromised to a great extent without seriously affecting the genome-wide termination function as well as the viability of Escherichia coli. These terminators function optimally only through a NusG-assisted recruitment and activation of Rho. Our data also indicate that at these terminators, Rho-EC-bound NusG interaction facilitates the isomerization of Rho into a translocase-competent form by stabilizing the interactions of mRNA with the secondary RNA binding site, thereby overcoming the defects of the primary RNA binding functions. PMID:25081210

Proving Program Termination With Matrix Weighted Digraphs

NASA Technical Reports Server (NTRS)

Dutle, Aaron

2015-01-01

Program termination analysis is an important task in logic and computer science. While determining if a program terminates is known to be undecidable in general, there has been a significant amount of attention given to finding sufficient and computationally practical conditions to prove termination. One such method takes a program and builds from it a matrix weighted digraph. These are directed graphs whose edges are labeled by square matrices with entries in {-1,0,1}, equipped with a nonstandard matrix multiplication. Certain properties of this digraph are known to imply the termination of the related program. In particular, termination of the program can be determined from the weights of the circuits in the digraph. In this talk, the motivation for addressing termination and how matrix weighted digraphs arise will be briefly discussed. The remainder of the talk will describe an efficient method for bounding the weights of a finite set of the circuits in a matrix weighted digraph, which allows termination of the related program to be deduced.

Downregulation of Ras C-terminal processing by JNK inhibition

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mouri, Wataru; Department of Neurosurgery, Yamagata University School of Medicine, Yamagata 990-9585; Biology Division, National Cancer Center Research Institute, Tokyo 104-0045

2008-06-27

After translation, Ras proteins undergo a series of modifications at their C-termini. This post-translational C-terminal processing is essential for Ras to become functional, but it remains unknown whether and how Ras C-terminal processing is regulated. Here we show that the C-terminal processing and subsequent plasma membrane localization of H-Ras as well as the activation of the downstream signaling pathways by H-Ras are prevented by JNK inhibition. Conversely, JNK activation by ultraviolet irradiation resulted in promotion of C-terminal processing of H-Ras. Furthermore, increased cell density promoted C-terminal processing of H-Ras most likely through an autocrine/paracrine mechanism, which was also blocked undermore » JNK-inhibited condition. Ras C-terminal processing was sensitive to JNK inhibition in the case of H- and N-Ras but not K-Ras, and in a variety of cell types. Thus, our results suggest for the first time that Ras C-terminal processing is a regulated mechanism in which JNK is involved.« less

End Joining-Mediated Gene Expression in Mammalian Cells Using PCR-Amplified DNA Constructs that Contain Terminator in Front of Promoter.

PubMed

Nakamura, Mikiko; Suzuki, Ayako; Akada, Junko; Tomiyoshi, Keisuke; Hoshida, Hisashi; Akada, Rinji

2015-12-01

Mammalian gene expression constructs are generally prepared in a plasmid vector, in which a promoter and terminator are located upstream and downstream of a protein-coding sequence, respectively. In this study, we found that front terminator constructs-DNA constructs containing a terminator upstream of a promoter rather than downstream of a coding region-could sufficiently express proteins as a result of end joining of the introduced DNA fragment. By taking advantage of front terminator constructs, FLAG substitutions, and deletions were generated using mutagenesis primers to identify amino acids specifically recognized by commercial FLAG antibodies. A minimal epitope sequence for polyclonal FLAG antibody recognition was also identified. In addition, we analyzed the sequence of a C-terminal Ser-Lys-Leu peroxisome localization signal, and identified the key residues necessary for peroxisome targeting. Moreover, front terminator constructs of hepatitis B surface antigen were used for deletion analysis, leading to the identification of regions required for the particle formation. Collectively, these results indicate that front terminator constructs allow for easy manipulations of C-terminal protein-coding sequences, and suggest that direct gene expression with PCR-amplified DNA is useful for high-throughput protein analysis in mammalian cells.

Psychotherapy Termination Practices with Older Adults: Impact of Patient and Therapist Characteristics.

PubMed

Sullivan, Daniel J; Zeff, Patricia; Zweig, Richard A

2018-02-06

The aims of this study were to survey clinicians' opinions regarding psychotherapy practices in mutual termination with a specified population (depressed older adult outpatients) and to examine the patient and therapist characteristics that may influence such practices. We surveyed psychologists' (N = 96) psychotherapy termination practices, using a hypothetical depressed older adult as a referent, to assess consensus on the appropriateness of various guidelines to termination and to examine whether these differ as a function of patient and therapist characteristics. Several practices were generally agreed to be "extremely appropriate" when terminating psychotherapy with older adults, including collaborating to determine the end date of treatment and discussing patient growth. Data also indicate that patient factors, such as personality pathology, and therapist factors, such as having an Integrative theoretical orientation were associated with differential endorsement of termination practices. Identification as a geropsychologist or working regularly with older adults were associated with a more cautious approach to termination. There is substantial consensus regarding many approaches to termination, but modifications might be appropriate depending on patient characteristics. Clinicians agree on a set of fundamental termination practices when working with older adults, but modify these based on orientation and diagnosis.

Superconducting Cable Termination

DOEpatents

Sinha, Uday K.; Tolbert, Jerry

2005-08-30

Disclosed is a termination that connects high temperature superconducting (HTS) cable immersed in pressurized liquid nitrogen to high voltage and neutral (shield) external bushings at ambient temperature and pressure. The termination consists of a splice between the HTS power (inner) and shield (outer) conductors and concentric copper pipes which are the conductors in the termination. There is also a transition from the dielectric tape insulator used in the HTS cable to the insulators used between and around the copper pipe conductors in the termination. At the warm end of the termination the copper pipes are connected via copper braided straps to the conventional warm external bushings which have low thermal stresses. This termination allows for a natural temperature gradient in the copper pipe conductors inside the termination which enables the controlled flashing of the pressurized liquid coolant (nitrogen) to the gaseous state. Thus the entire termination is near the coolant supply pressure and the high voltage and shield cold bushings, a highly stressed component used in most HTS cables, are eliminated. A sliding seal allows for cable contraction as it is cooled from room temperature to ˜72-82 K. Seals, static vacuum, and multi-layer superinsulation minimize radial heat leak to the environment.

Termination Patterns of Complex Partial Seizures: An Intracranial EEG Study

PubMed Central

Afra, Pegah; Jouny, Christopher C.; Bergey, Gregory K.

2015-01-01

Purpose While seizure onset patterns have been the subject of many reports, there have been few studies of seizure termination. In this study we report the incidence of synchronous and asynchronous termination patterns of partial seizures recorded with intracranial arrays. Methods Data were collected from patients with intractable complex partial seizures undergoing presurgical evaluations with intracranial electrodes. Patients with seizures originating from mesial temporal and neocortical regions were grouped into three groups based on patterns of seizure termination: synchronous only (So), asynchronous only (Ao), or mixed (S/A, with both synchronous and asynchronous termination patterns). Results 88% of the patients in the MT group had seizures with a synchronous pattern of termination exclusively (38%) or mixed (50%). 82% of the NC group had seizures with synchronous pattern of termination exclusively (52%) or mixed (30%). In the NC group, there was a significant difference of the range of seizure durations between So and Ao groups, with Ao exhibiting higher variability. Seizures with synchronous termination had low variability in both groups. Conclusions Synchronous seizure termination is a common pattern for complex partial seizures of both mesial temporal or neocortical onset. This may reflect stereotyped network behavior or dynamics at the seizure focus. PMID:26552555

76 FR 43675 - Termination of Department of Defense Federal Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-21

... DEPARTMENT OF DEFENSE Office of the Secretary Termination of Department of Defense Federal Advisory Committee AGENCY: Department of Defense. ACTION: Termination of Federal Advisory Committee....C. 552b), effective June 22, 2011 the Department of Defense gives notice that it is terminating the...

75 FR 41486 - Termination Settlement Proposal Forms-FAR (Standard Forms 1435 Through 1440)

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-16

.... 9000-0012] Termination Settlement Proposal Forms--FAR (Standard Forms 1435 Through 1440) AGENCY... concerning Termination Settlement Proposal Forms--FAR (Standard Forms 1435 through 1440). Public comments are... INFORMATION: A. Purpose The termination settlement proposal forms (Standard Forms 1435 through 1440) provide a...

40 CFR 721.10209 - Epoxy terminated, hydrolyzed trialkoxysilane and glycidyl ether of phenol-formaldehyde resin...

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 31 2014-07-01 2014-07-01 false Epoxy terminated, hydrolyzed... Epoxy terminated, hydrolyzed trialkoxysilane and glycidyl ether of phenol-formaldehyde resin (generic... identified generically as epoxy terminated, hydrolyzed trialkoxysilane and glycidyl ether of phenol...

40 CFR 721.10209 - Epoxy terminated, hydrolyzed trialkoxysilane and glycidyl ether of phenol-formaldehyde resin...

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 32 2013-07-01 2013-07-01 false Epoxy terminated, hydrolyzed... Epoxy terminated, hydrolyzed trialkoxysilane and glycidyl ether of phenol-formaldehyde resin (generic... identified generically as epoxy terminated, hydrolyzed trialkoxysilane and glycidyl ether of phenol...

40 CFR 721.10209 - Epoxy terminated, hydrolyzed trialkoxysilane and glycidyl ether of phenol-formaldehyde resin...

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 32 2012-07-01 2012-07-01 false Epoxy terminated, hydrolyzed... Epoxy terminated, hydrolyzed trialkoxysilane and glycidyl ether of phenol-formaldehyde resin (generic... identified generically as epoxy terminated, hydrolyzed trialkoxysilane and glycidyl ether of phenol...

40 CFR 721.10209 - Epoxy terminated, hydrolyzed trialkoxysilane and glycidyl ether of phenol-formaldehyde resin...

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Epoxy terminated, hydrolyzed... Epoxy terminated, hydrolyzed trialkoxysilane and glycidyl ether of phenol-formaldehyde resin (generic... identified generically as epoxy terminated, hydrolyzed trialkoxysilane and glycidyl ether of phenol...

42 CFR 408.90 - Termination of group billing arrangement.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 42 Public Health 2 2012-10-01 2012-10-01 false Termination of group billing arrangement. 408.90... SERVICES MEDICARE PROGRAM PREMIUMS FOR SUPPLEMENTARY MEDICAL INSURANCE Direct Remittance: Group Payment § 408.90 Termination of group billing arrangement. (a) A group billing arrangement may be terminated...

42 CFR 408.90 - Termination of group billing arrangement.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 2 2011-10-01 2011-10-01 false Termination of group billing arrangement. 408.90... SERVICES MEDICARE PROGRAM PREMIUMS FOR SUPPLEMENTARY MEDICAL INSURANCE Direct Remittance: Group Payment § 408.90 Termination of group billing arrangement. (a) A group billing arrangement may be terminated...

42 CFR 408.90 - Termination of group billing arrangement.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 42 Public Health 2 2013-10-01 2013-10-01 false Termination of group billing arrangement. 408.90... SERVICES MEDICARE PROGRAM PREMIUMS FOR SUPPLEMENTARY MEDICAL INSURANCE Direct Remittance: Group Payment § 408.90 Termination of group billing arrangement. (a) A group billing arrangement may be terminated...

46 CFR 8.450 - Termination of classification society authority.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Termination of classification society authority. 8.450... VESSEL INSPECTION ALTERNATIVES Alternate Compliance Program § 8.450 Termination of classification society authority. (a) The Coast Guard may terminate an authorization agreement with a classification society to...

10 CFR 600.244 - Termination for convenience.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Termination for convenience. 600.244 Section 600.244 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES Uniform... Requirements § 600.244 Termination for convenience. Except as provided in § 600.443 awards may be terminated in...

24 CFR 203.321 - Effect of termination.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Effect of termination. 203.321 Section 203.321 Housing and Urban Development Regulations Relating to Housing and Urban Development....321 Effect of termination. Upon termination of the contract of insurance, the obligation to pay any...

20 CFR 416.1334 - Termination due to death of recipient.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Termination due to death of recipient. 416... FOR THE AGED, BLIND, AND DISABLED Suspensions and Terminations § 416.1334 Termination due to death of... effective with the month after the month of death. ...

47 CFR 68.7 - Technical criteria for terminal equipment.

Code of Federal Regulations, 2010 CFR

2010-10-01

... (CONTINUED) CONNECTION OF TERMINAL EQUIPMENT TO THE TELEPHONE NETWORK General § 68.7 Technical criteria for... switched telephone network. (b) Technical criteria published by the Administrative Council for Terminal... network from harms caused by the connection of terminal equipment, subject to the appeal procedures in...

47 CFR 68.7 - Technical criteria for terminal equipment.

Code of Federal Regulations, 2011 CFR

2011-10-01

... (CONTINUED) CONNECTION OF TERMINAL EQUIPMENT TO THE TELEPHONE NETWORK General § 68.7 Technical criteria for... switched telephone network. (b) Technical criteria published by the Administrative Council for Terminal... network from harms caused by the connection of terminal equipment, subject to the appeal procedures in...

20 CFR 209.10 - Terminated employers' reports.

Code of Federal Regulations, 2010 CFR

2010-04-01

... RAILROAD EMPLOYERS' REPORTS AND RESPONSIBILITIES § 209.10 Terminated employers' reports. When an employer's status as an employer is terminated, a final report of creditable service and compensation shall be made... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Terminated employers' reports. 209.10 Section...

48 CFR 49.105-3 - Termination case file.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Termination case file. 49.105-3 Section 49.105-3 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT TERMINATION OF CONTRACTS General Principles 49.105-3 Termination case file. The TCO responsible...

10 CFR 1016.12 - Termination of security facility approval.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Termination of security facility approval. 1016.12 Section 1016.12 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) SAFEGUARDING OF RESTRICTED DATA Physical Security § 1016.12 Termination of security facility approval. Security facility approval will be terminated...

10 CFR 1016.12 - Termination of security facility approval.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Termination of security facility approval. 1016.12 Section 1016.12 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) SAFEGUARDING OF RESTRICTED DATA Physical Security § 1016.12 Termination of security facility approval. Security facility approval will be terminated...

10 CFR 20.1403 - Criteria for license termination under restricted conditions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 1 2012-01-01 2012-01-01 false Criteria for license termination under restricted conditions. 20.1403 Section 20.1403 Energy NUCLEAR REGULATORY COMMISSION STANDARDS FOR PROTECTION AGAINST RADIATION Radiological Criteria for License Termination § 20.1403 Criteria for license termination under...

10 CFR 20.1403 - Criteria for license termination under restricted conditions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 1 2010-01-01 2010-01-01 false Criteria for license termination under restricted conditions. 20.1403 Section 20.1403 Energy NUCLEAR REGULATORY COMMISSION STANDARDS FOR PROTECTION AGAINST RADIATION Radiological Criteria for License Termination § 20.1403 Criteria for license termination under...

10 CFR 20.1403 - Criteria for license termination under restricted conditions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 1 2014-01-01 2014-01-01 false Criteria for license termination under restricted conditions. 20.1403 Section 20.1403 Energy NUCLEAR REGULATORY COMMISSION STANDARDS FOR PROTECTION AGAINST RADIATION Radiological Criteria for License Termination § 20.1403 Criteria for license termination under...

10 CFR 20.1404 - Alternate criteria for license termination.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 1 2012-01-01 2012-01-01 false Alternate criteria for license termination. 20.1404 Section 20.1404 Energy NUCLEAR REGULATORY COMMISSION STANDARDS FOR PROTECTION AGAINST RADIATION Radiological Criteria for License Termination § 20.1404 Alternate criteria for license termination. Link to an...

10 CFR 20.1403 - Criteria for license termination under restricted conditions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 1 2011-01-01 2011-01-01 false Criteria for license termination under restricted conditions. 20.1403 Section 20.1403 Energy NUCLEAR REGULATORY COMMISSION STANDARDS FOR PROTECTION AGAINST RADIATION Radiological Criteria for License Termination § 20.1403 Criteria for license termination under...

10 CFR 20.1403 - Criteria for license termination under restricted conditions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 1 2013-01-01 2013-01-01 false Criteria for license termination under restricted conditions. 20.1403 Section 20.1403 Energy NUCLEAR REGULATORY COMMISSION STANDARDS FOR PROTECTION AGAINST RADIATION Radiological Criteria for License Termination § 20.1403 Criteria for license termination under...

48 CFR 49.402-3 - Procedure for default.

Code of Federal Regulations, 2010 CFR

2010-10-01

... contracting officer shall consider the following factors in determining whether to terminate a contract for... portion of the contract) is terminated; (4) That the supplies or services terminated may be purchased... of termination constitutes such decision, and that the contractor has the right to appeal such...

47 CFR 51.701 - Scope of transport and termination pricing rules.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 3 2010-10-01 2010-10-01 false Scope of transport and termination pricing... CARRIER SERVICES (CONTINUED) INTERCONNECTION Reciprocal Compensation for Transport and Termination of Telecommunications Traffic § 51.701 Scope of transport and termination pricing rules. (a) The provisions of this...

47 CFR 51.701 - Scope of transport and termination pricing rules.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 3 2011-10-01 2011-10-01 false Scope of transport and termination pricing... CARRIER SERVICES (CONTINUED) INTERCONNECTION Reciprocal Compensation for Transport and Termination of Telecommunications Traffic § 51.701 Scope of transport and termination pricing rules. (a) The provisions of this...

49 CFR 374.309 - Terminal facilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 5 2010-10-01 2010-10-01 false Terminal facilities. 374.309 Section 374.309... REGULATIONS Adequacy of Intercity Motor Common Carrier Passenger Service § 374.309 Terminal facilities. (a... attendants and be regularly patrolled. (b) Outside facilities. At terminals and stations that are closed when...

48 CFR 49.105-3 - Termination case file.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Termination case file. 49.105-3 Section 49.105-3 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT TERMINATION OF CONTRACTS General Principles 49.105-3 Termination case file. The TCO responsible...

48 CFR 49.105-3 - Termination case file.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Termination case file. 49.105-3 Section 49.105-3 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT TERMINATION OF CONTRACTS General Principles 49.105-3 Termination case file. The TCO responsible...

48 CFR 49.105-3 - Termination case file.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Termination case file. 49.105-3 Section 49.105-3 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT TERMINATION OF CONTRACTS General Principles 49.105-3 Termination case file. The TCO responsible...

48 CFR 49.105-3 - Termination case file.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Termination case file. 49.105-3 Section 49.105-3 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT TERMINATION OF CONTRACTS General Principles 49.105-3 Termination case file. The TCO responsible...

46 CFR 8.450 - Termination of classification society authority.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 1 2011-10-01 2011-10-01 false Termination of classification society authority. 8.450... VESSEL INSPECTION ALTERNATIVES Alternate Compliance Program § 8.450 Termination of classification society authority. (a) The Coast Guard may terminate an authorization agreement with a classification society to...

48 CFR 52.249-6 - Termination (Cost-Reimbursement).

Code of Federal Regulations, 2010 CFR

2010-10-01

...-Reimbursement). 52.249-6 Section 52.249-6 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION....249-6 Termination (Cost-Reimbursement). As prescribed in 49.503(a)(1), insert the following clause: Termination (Cost-Reimbursement) (MAY 2004) (a) The Government may terminate performance of work under this...

29 CFR 408.8 - Terminal trusteeship information report.

Code of Federal Regulations, 2010 CFR

2010-07-01

... LABOR-MANAGEMENT STANDARDS LABOR ORGANIZATION TRUSTEESHIP REPORTS § 408.8 Terminal trusteeship information report. There shall be filed at the same time that the terminal trusteeship financial report is... 29 Labor 2 2010-07-01 2010-07-01 false Terminal trusteeship information report. 408.8 Section 408...

26 CFR 1.1362-5 - Election after termination.

Code of Federal Regulations, 2010 CFR

2010-04-01

... termination. (a) In general. Absent the Commissioner's consent, an S corporation whose election has terminated... as described in section 1362(g). However, the Commissioner may permit the corporation to make a new... termination was not reasonably within the control of the corporation or shareholders having a substantial...

20 CFR 416.1334 - Termination due to death of recipient.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false Termination due to death of recipient. 416... FOR THE AGED, BLIND, AND DISABLED Suspensions and Terminations § 416.1334 Termination due to death of... effective with the month after the month of death. ...

20 CFR 416.1334 - Termination due to death of recipient.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 20 Employees' Benefits 2 2011-04-01 2011-04-01 false Termination due to death of recipient. 416... FOR THE AGED, BLIND, AND DISABLED Suspensions and Terminations § 416.1334 Termination due to death of... effective with the month after the month of death. ...

44 CFR 13.44 - Termination for convenience.

Code of Federal Regulations, 2010 CFR

2010-10-01

.... 13.44 Section 13.44 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY... § 13.44 Termination for convenience. Except as provided in § 13.43 awards may be terminated in whole or... upon written notification to the awarding agency, setting forth the reasons for such termination, the...

77 FR 61550 - Airworthiness Directives; The Boeing Company Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-10

... inspection required by AD 2003-14-06 only terminates the external eddy current inspections required by..., which should not terminate the detailed or eddy current inspection, as specified. We find that... terminating action ``before the effective date of this AD'' terminates the eddy current inspections required...

Channel and terminal description of the ACTS mobile terminal

NASA Technical Reports Server (NTRS)

Abbe, B. S.; Agan, M. J.; Girardey, C. C.; Jedrey, T. C.

1994-01-01

The Advanced Communications Technology Satellite (ACTS) Mobile Terminal (AMT) is a proof-of-concept K/Ka-band mobile satellite communications terminal under development by NASA at JPL. Currently the AMT is undergoing systems integration and testing in preparation for a July 1993 ACTS launch and the subsequent commencement of mobile experiments in the fall of 1993. The AMT objectives are presented, followed by a discussion of the AMT communications channel and the mobile terminal's design and performance.

Channel and terminal description of the ACTS mobile terminal

NASA Technical Reports Server (NTRS)

Abbe, B. S.; Agan, M. J.; Girardey, C. C.; Jedrey, T. C.

1993-01-01

The Advanced Communications Technology Satellite (ACTS) Mobile Terminal (AMT) is a proof-of-concept K/Ka-band mobile satellite communications terminal under development by NASA at JPL. Currently the AMT is undergoing system integration and test in preparation for a July 1993 ACTS launch and the subsequent commencement of mobile experiments in the fall of 1993. The AMT objectives are presented followed by a discussion of the AMT communications channel and mobile terminal design and performance.

Characterization of five terminator regions that increase the protein yield of a transgene in Saccharomyces cerevisiae.

PubMed

Ito, Yoichiro; Yamanishi, Mamoru; Ikeuchi, Akinori; Imamura, Chie; Tokuhiro, Kenro; Kitagawa, Takao; Matsuyama, Takashi

2013-12-01

Strong terminator regions could be used to improve metabolically engineered yeasts by increasing the target enzyme protein yields above those achieved with traditional terminator regions. We recently identified five strong terminator regions (RPL41Bt, RPL15At, DIT1t, RPL3t, and IDP1t) in a comprehensive analysis of Saccharomyces cerevisiae. The effect of the terminator regions was analyzed by measuring the protein production of a linked transgene, and was shown to be twice that of a traditional terminator region (PGK1t). Here, we investigated whether the activity of the terminator regions is affected by exchange of a strong promoter or reporter in the linked transgene, carbon source for cell growth, stress factors, host yeast strain, or stage of the growth phase. Our results indicate that the activities of all five terminator regions were twice that of PGK1t in all conditions tested. In addition, we demonstrated that the strong activity of these terminator regions could be used to improve secretory production of endoglucanase II derived from Tricoderma ressei, and that the DIT1t strain was the best of the five strains for this purpose. We therefore propose that DIT1t, and the four other terminator regions, could be applied to the development of improved metabolically engineered yeasts. Copyright © 2013 Elsevier B.V. All rights reserved.

Acetylation within the N- and C-Terminal Domains of Src Regulates Distinct Roles of STAT3-Mediated Tumorigenesis.

PubMed

Huang, Chao; Zhang, Zhe; Chen, Lihan; Lee, Hank W; Ayrapetov, Marina K; Zhao, Ting C; Hao, Yimei; Gao, Jinsong; Yang, Chunzhang; Mehta, Gautam U; Zhuang, Zhengping; Zhang, Xiaoren; Hu, Guohong; Chin, Y Eugene

2018-06-01

Posttranslational modifications of mammalian c-Src N-terminal and C-terminal domains regulate distinct functions. Myristoylation of G 2 controls its cell membrane association and phosphorylation of Y419/Y527 controls its activation or inactivation, respectively. We provide evidence that Src-cell membrane association-dissociation and catalytic activation-inactivation are both regulated by acetylation. In EGF-treated cells, CREB binding protein (CBP) acetylates an N-terminal lysine cluster (K5, K7, and K9) of c-Src to promote dissociation from the cell membrane. CBP also acetylates the C-terminal K401, K423, and K427 of c-Src to activate intrinsic kinase activity for STAT3 recruitment and activation. N-terminal domain phosphorylation (Y14, Y45, and Y68) of STAT3 by c-Src activates transcriptionally active dimers of STAT3. Moreover, acetyl-Src translocates into nuclei, where it forms the Src-STAT3 enhanceosome for gene regulation and cancer cell proliferation. Thus, c-Src acetylation in the N-terminal and C-terminal domains play distinct roles in Src activity and regulation. Significance: CBP-mediated acetylation of lysine clusters in both the N-terminal and C-terminal regions of c-Src provides additional levels of control over STAT3 transcriptional activity. Cancer Res; 78(11); 2825-38. ©2018 AACR . ©2018 American Association for Cancer Research.

Experiences and attitudes of patients with terminal cancer and their family caregivers toward the disclosure of terminal illness.

PubMed

Yun, Young Ho; Kwon, Yong Chol; Lee, Myung Kyung; Lee, Woo Jin; Jung, Kyung Hae; Do, Young Rok; Kim, Samyong; Heo, Dae Seog; Choi, Jong Soo; Park, Sang Yoon

2010-04-10

PURPOSE We investigated the experiences of cancer patients and their family caregivers who became aware that the cancer was terminal, how they became aware, and how they felt about disclosure of the information. PATIENTS AND METHODS In this cohort study, we administered questionnaires to 619 consecutive patients determined by physicians to be terminally ill and to their family caregivers. RESULTS A total of 481 patients and 381 family caregivers completed the questionnaire. A majority of patients (58.0%) and caregivers (83.4%) were aware of the patient's terminal status. Approximately 28% of patients and 23% of caregivers reported that they guessed it from the patient's worsening condition. The patient group was more likely than the caregiver group (78.6% v 69.6%) to prefer that patients be informed of their terminal status. Patients informed of their terminal diagnosis had a significantly better quality of life and fewer symptoms and had a lower rate of emotional distress than patients who guessed it from their worsening condition. Younger patients and patients who paid the treatment costs themselves were significantly more likely to want to be told when their illness was terminal. If the patient paid the treatment cost and was employed at the time of the cancer diagnosis, the family caregivers were more likely to prefer disclosure of terminal illness. CONCLUSION Most patients with terminal cancer and their family caregivers preferred disclosure, and patients who knew of their terminal diagnosis had a lower rate of emotional distress and a higher health-related quality of life.

Receiving Versus Being Denied a Pregnancy Termination and Subsequent Alcohol Use: A Longitudinal Study

PubMed Central

Roberts, Sarah C. M.; Delucchi, Kevin; Wilsnack, Sharon C.; Foster, Diana Greene

2015-01-01

Aim Research finds women who terminate pregnancies are at risk of subsequent problematic alcohol use, but methodological and conceptual problems are common. This study examines the relationship between receiving versus being denied termination and subsequent alcohol use. Methods Data are from a prospective, longitudinal study of US women seeking pregnancy terminations. Participants presented just before a facility's gestational limit and received terminations (Near Limits, n = 452) or just beyond the limit and were denied terminations (Turnaways, n = 231). Results Groups did not differ in alcohol use before pregnancy recognition. One week after termination-seeking (Turnaways still pregnant, Near Limits not), Turnaways had lower odds of any and binge alcohol use, but did not differ on 1+ problem symptoms. Over 2.5 years, both Near Limits and Turnaways increased any and binge alcohol use, with Turnaways increasing more rapidly. The groups did not converge again on any or binge use. For Near Limits, any alcohol use surpassed the pre-pregnancy recognition level, but binge use did not. Changes in problem symptoms over time were not evident for either group. Conclusion While women who had a termination were more likely to report any and binge alcohol use than women who had a child, this difference was due to a reduction in consumption among women having the child rather than an increase in consumption among women having a termination. Thus, assertions that having a termination leads women to increase alcohol use to cope with having had a termination are not supported. PMID:25787011

25 CFR 11.1114 - Termination.

Code of Federal Regulations, 2010 CFR

2010-04-01

... functions; (iii) The parent(s) has subjected the minor to willful and repeated acts of sexual abuse; (iv... Minor-in-Need-of-Care Procedure § 11.1114 Termination. (a) Parental rights to a child may be terminated by the children's court according to the procedures in this section. (b) Proceedings to terminate...

9 CFR 2.12 - Termination of a license.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Termination of a license. 2.12 Section 2.12 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Licensing § 2.12 Termination of a license. A license may be terminated...

76 FR 71022 - Dicloran; Cancellation Order for Amendment To Terminate Use on Potatoes

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-16

... for Amendment To Terminate Use on Potatoes AGENCY: Environmental Protection Agency (EPA). ACTION: Notice. SUMMARY: This notice announces EPA's order for the amendment to terminate use on potatoes... the registrant listed in Table 2 of Unit II. to voluntarily amend to terminate DCNA use on potatoes...

7 CFR 3016.44 - Termination for convenience.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 15 2010-01-01 2010-01-01 false Termination for convenience. 3016.44 Section 3016.44 Agriculture Regulations of the Department of Agriculture (Continued) OFFICE OF THE CHIEF FINANCIAL OFFICER... Termination for convenience. Except as provided in § 3016.43 awards may be terminated in whole or in part only...

36 CFR 1207.44 - Termination for convenience.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Termination for convenience. 1207.44 Section 1207.44 Parks, Forests, and Public Property NATIONAL ARCHIVES AND RECORDS... Termination for convenience. Except as provided in § 1207.43 awards may be terminated in whole or in part only...

41 CFR 105-71.144 - Termination for convenience.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Termination for convenience. 105-71.144 Section 105-71.144 Public Contracts and Property Management Federal Property... Termination for convenience. Except as provided in § 105-71.143 awards may be terminated in whole or in part...

45 CFR 1157.44 - Termination for convenience.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 3 2010-10-01 2010-10-01 false Termination for convenience. 1157.44 Section 1157.44 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE ARTS... Enforcement § 1157.44 Termination for convenience. Except as provided in § 1157.43 awards may be terminated in...

29 CFR 97.44 - Termination for convenience.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 1 2010-07-01 2010-07-01 true Termination for convenience. 97.44 Section 97.44 Labor Office of the Secretary of Labor UNIFORM ADMINISTRATIVE REQUIREMENTS FOR GRANTS AND COOPERATIVE... § 97.44 Termination for convenience. Except as provided in § 97.43 awards may be terminated in whole or...

45 CFR 602.44 - Termination for convenience.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 3 2010-10-01 2010-10-01 false Termination for convenience. 602.44 Section 602.44 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION UNIFORM... Requirements § 602.44 Termination for convenience. Except as provided in § 602.43 awards may be terminated in...

49 CFR 18.44 - Termination for convenience.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Termination for convenience. 18.44 Section 18.44 Transportation Office of the Secretary of Transportation UNIFORM ADMINISTRATIVE REQUIREMENTS FOR GRANTS AND... Enforcement § 18.44 Termination for convenience. Except as provided in § 18.43 awards may be terminated in...

29 CFR 100.622 - Termination of collection action.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 2 2010-07-01 2010-07-01 false Termination of collection action. 100.622 Section 100.622 Labor Regulations Relating to Labor NATIONAL LABOR RELATIONS BOARD ADMINISTRATIVE REGULATIONS Debt Collection Procedures § 100.622 Termination of collection action. Before terminating collection activity, the NLRB will have pursued all appropriate...

29 CFR 100.622 - Termination of collection action.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 2 2011-07-01 2011-07-01 false Termination of collection action. 100.622 Section 100.622 Labor Regulations Relating to Labor NATIONAL LABOR RELATIONS BOARD ADMINISTRATIVE REGULATIONS Debt Collection Procedures § 100.622 Termination of collection action. Before terminating collection activity, the NLRB will have pursued all appropriate...

45 CFR 155.735 - Termination of coverage.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Functions: Small Business Health Options Program (SHOP) § 155.735 Termination of coverage. (a) General requirements. The SHOP must determine the timing, form, and manner in which coverage in a QHP may be terminated. (b) Termination of employer group health coverage at the request of the employer. (1) The SHOP must...

45 CFR 155.735 - Termination of coverage.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Functions: Small Business Health Options Program (SHOP) § 155.735 Termination of coverage. (a) General requirements. The SHOP must determine the timing, form, and manner in which coverage in a QHP may be terminated. (b) Termination of employer group health coverage at the request of the employer. (1) The SHOP must...

77 FR 35705 - Notice of Submission of Proposed Information Collection to OMB Request for Termination of...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-14

... Proposed Information Collection to OMB Request for Termination of Multifamily Mortgage Insurance AGENCY... mutually agree to terminate the HUD multifamily mortgage insurance. DATES: Comments Due Date: July 16, 2012... following information: Title of Proposal: Request for Termination of Multifamily Mortgage Insurance. OMB...

20 CFR 416.1332 - Termination of benefit for disabled individual: Exception.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false Termination of benefit for disabled... SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Suspensions and Terminations § 416.1332 Termination of benefit for disabled individual: Exception. Special SSI cash benefits (see § 416.261) will be...

20 CFR 416.1332 - Termination of benefit for disabled individual: Exception.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 20 Employees' Benefits 2 2013-04-01 2013-04-01 false Termination of benefit for disabled... SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Suspensions and Terminations § 416.1332 Termination of benefit for disabled individual: Exception. Special SSI cash benefits (see § 416.261) will be...

20 CFR 416.1332 - Termination of benefit for disabled individual: Exception.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Termination of benefit for disabled... SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Suspensions and Terminations § 416.1332 Termination of benefit for disabled individual: Exception. Special SSI cash benefits (see § 416.261) will be...

20 CFR 416.1332 - Termination of benefit for disabled individual: Exception.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 20 Employees' Benefits 2 2012-04-01 2012-04-01 false Termination of benefit for disabled... SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Suspensions and Terminations § 416.1332 Termination of benefit for disabled individual: Exception. Special SSI cash benefits (see § 416.261) will be...

20 CFR 416.1332 - Termination of benefit for disabled individual: Exception.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 20 Employees' Benefits 2 2011-04-01 2011-04-01 false Termination of benefit for disabled... SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Suspensions and Terminations § 416.1332 Termination of benefit for disabled individual: Exception. Special SSI cash benefits (see § 416.261) will be...

45 CFR 1210.3-6 - Appeal of termination.

Code of Federal Regulations, 2011 CFR

2011-10-01

... SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-6 Appeal of termination. (a) Appeal to Regional Director. A Volunteer has 10 days from... Director. The appeal must be in writing and specify the reasons for the Volunteer's disagreement with the...

45 CFR 1210.3-6 - Appeal of termination.

Code of Federal Regulations, 2012 CFR

2012-10-01

... SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-6 Appeal of termination. (a) Appeal to Regional Director. A Volunteer has 10 days from... Director. The appeal must be in writing and specify the reasons for the Volunteer's disagreement with the...

45 CFR 1210.3-6 - Appeal of termination.

Code of Federal Regulations, 2014 CFR

2014-10-01

... SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-6 Appeal of termination. (a) Appeal to Regional Director. A Volunteer has 10 days from... Director. The appeal must be in writing and specify the reasons for the Volunteer's disagreement with the...

45 CFR 1210.3-6 - Appeal of termination.

Code of Federal Regulations, 2013 CFR

2013-10-01

... SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-6 Appeal of termination. (a) Appeal to Regional Director. A Volunteer has 10 days from... Director. The appeal must be in writing and specify the reasons for the Volunteer's disagreement with the...

78 FR 55121 - Proposed Submission of Information Collection for OMB Review; Comment Request; Termination of...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-09

...; Comment Request; Termination of Single-Employer Plans, Missing Participants AGENCY: Pension Benefit... information in its regulations on Termination of Single-Employer Plans and Missing Participants, and... missing participants (29 CFR part 4050). When PBGC conducts post-termination audits, it sometimes finds...

The PLATO IV Communications System.

ERIC Educational Resources Information Center

Sherwood, Bruce Arne; Stifle, Jack

The PLATO IV computer-based educational system contains its own communications hardware and software for operating plasma-panel graphics terminals. Key echoing is performed by the central processing unit: every key pressed at a terminal passes through the entire system before anything appears on the terminal's screen. Each terminal is guaranteed…

48 CFR 49.603-1 - Fixed-price contracts-complete termination.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Contractor certifies that all contract termination inventory (including scrap) has been retained or acquired... certificate stating (i) that all subcontract termination inventory (including scrap) has been retained or... certifies that all items of termination inventory, the costs of which were used in arriving at the amount of...

48 CFR 49.603-2 - Fixed-price contracts-partial termination.

Code of Federal Regulations, 2010 CFR

2010-10-01

... certifies that all contract termination inventory (including scrap) has been retained or acquired by the... stating (i) that all subcontract termination inventory (including scrap) has been retained or acquired by... certifies that all items of termination inventory, the costs of which were used in arriving at the amount of...

48 CFR 49.603-3 - Cost-reimbursement contracts-complete termination, if settlement includes cost.

Code of Federal Regulations, 2010 CFR

2010-10-01

... following: (1) The Contractor certifies that all contract termination inventory (including scrap) has been... the Contractor a certificate stating (i) that all subcontract termination inventory (including scrap... in its proposal. (3) The Contractor certifies that all items of termination inventory, the costs of...

47 CFR 68.110 - Compatibility of the public switched telephone network and terminal equipment.

Code of Federal Regulations, 2011 CFR

2011-10-01

... network and terminal equipment. 68.110 Section 68.110 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES (CONTINUED) CONNECTION OF TERMINAL EQUIPMENT TO THE TELEPHONE NETWORK Conditions on Use of Terminal Equipment § 68.110 Compatibility of the public switched telephone network and...

47 CFR 68.110 - Compatibility of the public switched telephone network and terminal equipment.

Code of Federal Regulations, 2010 CFR

2010-10-01

... network and terminal equipment. 68.110 Section 68.110 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES (CONTINUED) CONNECTION OF TERMINAL EQUIPMENT TO THE TELEPHONE NETWORK Conditions on Use of Terminal Equipment § 68.110 Compatibility of the public switched telephone network and...

38 CFR 17.370 - Termination of payments.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 1 2014-07-01 2014-07-01 false Termination of payments. 17.370 Section 17.370 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS MEDICAL Grants to the Republic of the Philippines § 17.370 Termination of payments. Payments may be terminated if...

38 CFR 17.370 - Termination of payments.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 1 2012-07-01 2012-07-01 false Termination of payments. 17.370 Section 17.370 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS MEDICAL Grants to the Republic of the Philippines § 17.370 Termination of payments. Payments may be terminated if...

38 CFR 17.370 - Termination of payments.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 1 2013-07-01 2013-07-01 false Termination of payments. 17.370 Section 17.370 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS MEDICAL Grants to the Republic of the Philippines § 17.370 Termination of payments. Payments may be terminated if...

38 CFR 17.370 - Termination of payments.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 1 2011-07-01 2011-07-01 false Termination of payments. 17.370 Section 17.370 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS MEDICAL Grants to the Republic of the Philippines § 17.370 Termination of payments. Payments may be terminated if...

38 CFR 17.370 - Termination of payments.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 1 2010-07-01 2010-07-01 false Termination of payments. 17.370 Section 17.370 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS MEDICAL Grants to the Republic of the Philippines § 17.370 Termination of payments. Payments may be terminated if...

77 FR 46995 - Termination, Limited Reductions in Funding, and Debarment Procedures; Recompetition; Enforcement...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-07

... LEGAL SERVICES CORPORATION 45 CFR Parts 1606, 1618, and 1623 Termination, Limited Reductions in...- cost procedures (45 CFR part 1630). LSC has also adopted grant termination procedures (45 CFR part 1606... reduce a recipient's funding by less than five percent without using the 1606 termination procedures...

15 CFR 930.115 - Termination of mediation.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false Termination of mediation. 930.115... MANAGEMENT FEDERAL CONSISTENCY WITH APPROVED COASTAL MANAGEMENT PROGRAMS Secretarial Mediation § 930.115 Termination of mediation. Mediation shall terminate: (a) At any time the Federal and State agencies agree to a...

24 CFR 982.454 - Termination of HAP contract: Insufficient funding.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Termination of HAP contract... Assistance Payments Contract and Owner Responsibility § 982.454 Termination of HAP contract: Insufficient funding. The PHA may terminate the HAP contract if the PHA determines, in accordance with HUD requirements...

45 CFR 1210.4 - Early termination procedures for National Grant Trainees and Volunteers.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 4 2011-10-01 2011-10-01 false Early termination procedures for National Grant... (Continued) CORPORATION FOR NATIONAL AND COMMUNITY SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES National Grant Trainees and Volunteers § 1210.4 Early termination procedures for...

45 CFR 1210.4 - Early termination procedures for National Grant Trainees and Volunteers.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 4 2010-10-01 2010-10-01 false Early termination procedures for National Grant... (Continued) CORPORATION FOR NATIONAL AND COMMUNITY SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES National Grant Trainees and Volunteers § 1210.4 Early termination procedures for...

45 CFR 96.92 - Termination of funding.

Code of Federal Regulations, 2014 CFR

2014-10-01

... the Community Services Block Grant Act that it will terminate present or future funding of any... the record prior to terminating funding. If a review by the Secretary of the State's final decision to... of notification by the State of its final decision to terminate funding. The Department will confirm...

45 CFR 96.92 - Termination of funding.

Code of Federal Regulations, 2012 CFR

2012-10-01

... the Community Services Block Grant Act that it will terminate present or future funding of any... the record prior to terminating funding. If a review by the Secretary of the State's final decision to... of notification by the State of its final decision to terminate funding. The Department will confirm...

45 CFR 96.92 - Termination of funding.

Code of Federal Regulations, 2011 CFR

2011-10-01

... the Community Services Block Grant Act that it will terminate present or future funding of any... the record prior to terminating funding. If a review by the Secretary of the State's final decision to... of notification by the State of its final decision to terminate funding. The Department will confirm...

45 CFR 96.92 - Termination of funding.

Code of Federal Regulations, 2013 CFR

2013-10-01

... the Community Services Block Grant Act that it will terminate present or future funding of any... the record prior to terminating funding. If a review by the Secretary of the State's final decision to... of notification by the State of its final decision to terminate funding. The Department will confirm...

5 CFR 837.403 - Termination of annuity during reemployment.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 5 Administrative Personnel 2 2010-01-01 2010-01-01 false Termination of annuity during... Termination of annuity during reemployment. (a) Agency action. When a reemployed disability annuitant is found recovered from disability or restored to earning capacity by OPM, OPM shall terminate the annuity as of the...

9 CFR 2.12 - Termination of a license.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Termination of a license. 2.12 Section 2.12 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Licensing § 2.12 Termination of a license. A license may be terminated...

9 CFR 2.12 - Termination of a license.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Termination of a license. 2.12 Section 2.12 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Licensing § 2.12 Termination of a license. A license may be terminated...

9 CFR 2.12 - Termination of a license.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Termination of a license. 2.12 Section 2.12 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Licensing § 2.12 Termination of a license. A license may be terminated...

9 CFR 2.12 - Termination of a license.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Termination of a license. 2.12 Section 2.12 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Licensing § 2.12 Termination of a license. A license may be terminated...

47 CFR 11.13 - Emergency Action Notification (EAN) and Emergency Action Termination (EAT).

Code of Federal Regulations, 2010 CFR

2010-10-01

... Emergency Action Termination (EAT). 11.13 Section 11.13 Telecommunication FEDERAL COMMUNICATIONS COMMISSION... Action Termination (EAT). (a) The Emergency Action Notification (EAN) is the notice to all EAS... Emergency Action Termination (EAT) is the notice to all EAS Participants and to the general public that the...

22 CFR 309.22 - Termination of collection.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 22 Foreign Relations 2 2011-04-01 2009-04-01 true Termination of collection. 309.22 Section 309.22 Foreign Relations PEACE CORPS DEBT COLLECTION Salary Offset § 309.22 Termination of collection. Termination of collection action shall be made in accordance with the standards set forth in the FCCS (31 CFR...

22 CFR 309.22 - Termination of collection.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 22 Foreign Relations 2 2012-04-01 2009-04-01 true Termination of collection. 309.22 Section 309.22 Foreign Relations PEACE CORPS DEBT COLLECTION Salary Offset § 309.22 Termination of collection. Termination of collection action shall be made in accordance with the standards set forth in the FCCS (31 CFR...

22 CFR 309.22 - Termination of collection.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 2 2010-04-01 2010-04-01 true Termination of collection. 309.22 Section 309.22 Foreign Relations PEACE CORPS DEBT COLLECTION Salary Offset § 309.22 Termination of collection. Termination of collection action shall be made in accordance with the standards set forth in the FCCS (31 CFR...

22 CFR 309.22 - Termination of collection.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 22 Foreign Relations 2 2013-04-01 2009-04-01 true Termination of collection. 309.22 Section 309.22 Foreign Relations PEACE CORPS DEBT COLLECTION Salary Offset § 309.22 Termination of collection. Termination of collection action shall be made in accordance with the standards set forth in the FCCS (31 CFR...

22 CFR 309.22 - Termination of collection.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 22 Foreign Relations 2 2014-04-01 2014-04-01 false Termination of collection. 309.22 Section 309.22 Foreign Relations PEACE CORPS DEBT COLLECTION Salary Offset § 309.22 Termination of collection. Termination of collection action shall be made in accordance with the standards set forth in the FCCS (31 CFR...

76 FR 18388 - Single-Employer Plan Termination Initiated by PBGC

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-04

... PENSION BENEFIT GUARANTY CORPORATION 29 CFR Part 4042 Single-Employer Plan Termination Initiated... of ERISA that a plan should be terminated, an affected party with respect to the plan may make a request to PBGC for the administrative record of PBGC's determination that the plan should be terminated...

49 CFR 22.33 - Termination of participation in the STLP.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Termination of participation in the STLP. 22.33 Section 22.33 Transportation Office of the Secretary of Transportation SHORT-TERM LENDING PROGRAM (STLP) Participating Lenders § 22.33 Termination of participation in the STLP. (a) DOT OSDBU Termination for...

34 CFR 682.608 - Termination of a school's lending eligibility.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 3 2010-07-01 2010-07-01 false Termination of a school's lending eligibility. 682.608..., Standards, and Payments for Participating Schools § 682.608 Termination of a school's lending eligibility... subsequent holder. (c) Exception based on hardship. The Secretary does not terminate a school's lending...

12 CFR 611.1235 - Plan of termination-distribution.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Plan of termination-distribution. 611.1235 Section 611.1235 Banks and Banking FARM CREDIT ADMINISTRATION FARM CREDIT SYSTEM ORGANIZATION Termination... resolution. Not more than 14 days before mailing the plan of termination to your equity holders, your board...

12 CFR 611.1220 - Termination resolution.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Termination resolution. 611.1220 Section 611.1220 Banks and Banking FARM CREDIT ADMINISTRATION FARM CREDIT SYSTEM ORGANIZATION Termination of System Institution Status § 611.1220 Termination resolution. No more than 1 week before you submit your plan of...

29 CFR 4043.24 - Termination or partial termination.

Code of Federal Regulations, 2012 CFR

2012-07-01

... REPORTABLE EVENTS AND CERTAIN OTHER NOTIFICATION REQUIREMENTS Post-Event Notice of Reportable Events § 4043.24 Termination or partial termination. (a) Reportable event. A reportable event occurs when the... within the meaning of section 411(d)(3) of the Code. (b) Waivers. Notice is waived for this event. ...

29 CFR 4043.24 - Termination or partial termination.

Code of Federal Regulations, 2014 CFR

2014-07-01

... REPORTABLE EVENTS AND CERTAIN OTHER NOTIFICATION REQUIREMENTS Post-Event Notice of Reportable Events § 4043.24 Termination or partial termination. (a) Reportable event. A reportable event occurs when the... within the meaning of section 411(d)(3) of the Code. (b) Waivers. Notice is waived for this event. ...

29 CFR 4043.24 - Termination or partial termination.

Code of Federal Regulations, 2013 CFR

2013-07-01

... REPORTABLE EVENTS AND CERTAIN OTHER NOTIFICATION REQUIREMENTS Post-Event Notice of Reportable Events § 4043.24 Termination or partial termination. (a) Reportable event. A reportable event occurs when the... within the meaning of section 411(d)(3) of the Code. (b) Waivers. Notice is waived for this event. ...

29 CFR 4043.24 - Termination or partial termination.

Code of Federal Regulations, 2011 CFR

2011-07-01

... REPORTABLE EVENTS AND CERTAIN OTHER NOTIFICATION REQUIREMENTS Post-Event Notice of Reportable Events § 4043.24 Termination or partial termination. (a) Reportable event. A reportable event occurs when the... within the meaning of section 411(d)(3) of the Code. (b) Waivers. Notice is waived for this event. ...

29 CFR 4043.24 - Termination or partial termination.

Code of Federal Regulations, 2010 CFR

2010-07-01

... REPORTABLE EVENTS AND CERTAIN OTHER NOTIFICATION REQUIREMENTS Post-Event Notice of Reportable Events § 4043.24 Termination or partial termination. (a) Reportable event. A reportable event occurs when the... within the meaning of section 411(d)(3) of the Code. (b) Waivers. Notice is waived for this event. ...

29 CFR 408.7 - Terminal trusteeship financial report.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 2 2010-07-01 2010-07-01 false Terminal trusteeship financial report. 408.7 Section 408.7...-MANAGEMENT STANDARDS LABOR ORGANIZATION TRUSTEESHIP REPORTS § 408.7 Terminal trusteeship financial report... a terminal financial report, and one copy, with the Office of Labor-Management Standards, on Form LM...

20 CFR 416.1333 - Termination at the request of the recipient.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false Termination at the request of the recipient. 416.1333 Section 416.1333 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Suspensions and Terminations § 416.1333 Termination at the...

13 CFR 124.303 - What is termination?

Code of Federal Regulations, 2010 CFR

2010-01-01

... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What is termination? 124.303 Section 124.303 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT... § 124.303 What is termination? (a) SBA may terminate the participation of a concern in the 8(a) BD...

TPS1 terminator increases mRNA and protein yield in a Saccharomyces cerevisiae expression system.

PubMed

Yamanishi, Mamoru; Katahira, Satoshi; Matsuyama, Takashi

2011-01-01

Both terminators and promoters regulate gene expression. In Saccharomyces cerevisiae, the TPS1 terminator (TPS1t), coupled to a gene encoding a fluorescent protein, produced more transgenic mRNA and protein than did similar constructs containing other terminators, such as CYC1t, TDH3t, and PGK1t. This suggests that TPS1t can be used as a general terminator in the development of metabolically engineered yeast in high-yield systems.

System issues and considerations associated with design of ground mobile strategic satellite communication terminals

NASA Astrophysics Data System (ADS)

Poliakon, J. A.

The current national defense doctrine calls for increased use of ground mobile strategic satellite communication earth terminals. This paper discusses some of the key communication terminal system issues and considerations associated with the design of nuclear hardened strategic ground mobile earth terminals. It considers system requirements such as nuclear hardness, rapid mobility, low profile, signal interference resistance, high availability, and long term independent operation. It also discusses impacts and implications associated with these requirements when imposed on a satellite earth terminal. It gives special focus to the ramifications of imposing mobility on earth terminals and its relationship to the system design approach used to arrive at an optimal system solution.

Voltammetric and impedance behaviours of surface-treated nano-crystalline diamond film electrodes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, F. B.; Jing, B.; Cui, Y.

2015-04-15

The electrochemical performances of hydrogen- and oxygen-terminated nano-crystalline diamond film electrodes were investigated by cyclic voltammetry and AC impedance spectroscopy. In addition, the surface morphologies, phase structures, and chemical states of the two diamond films were analysed by scanning probe microscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy, respectively. The results indicated that the potential window is narrower for the hydrogen-terminated nano-crystalline diamond film than for the oxygen-terminated one. The diamond film resistance and capacitance of oxygen-terminated diamond film are much larger than those of the hydrogen-terminated diamond film, and the polarization resistances and double-layer capacitance corresponding to oxygen-terminated diamond filmmore » are both one order of magnitude larger than those corresponding to the hydrogen-terminated diamond film. The electrochemical behaviours of the two diamond film electrodes are discussed.« less

Clients' reasons for terminating psychotherapy: a quantitative and qualitative inquiry.

PubMed

Roe, David; Dekel, Rachel; Harel, Galit; Fennig, Shmuel

2006-12-01

To study private-practice clients' perspective on reasons for psychotherapy termination and how these are related to demographic and treatment variables and to satisfaction with therapy. Eighty-four persons who had been in extended private-practice psychotherapy which ended in the preceding three years participated in the study. Mean number of months in treatment was 27.70 (SD = 18.70). Assessment included rating scales and open-ended questions assessing demographic variables, reasons for terminating therapy, and satisfaction with therapy. Quantitative results revealed that the most frequent reasons for termination were accomplishment of goals, circumstantial constraints and dissatisfaction with therapy, and that client satisfaction was positively related to positive reasons for termination. Qualitative results revealed two additional frequently mentioned reasons for termination: the client's need for independence and the client's involvement in new meaningful relationships. Findings suggest that psychotherapy termination may at times be required to facilitate the pursuit of personally meaningful goals.

Cloning and determination of the transcription termination site of ribosomal RNA gene of the mouse.

PubMed Central

Kominami, R; Mishima, Y; Urano, Y; Sakai, M; Muramatsu, M

1982-01-01

A Eco RI 6.6 kb DNA fragment containing the 3'-end of 28S ribosomal RNA gene of the mouse was detected by Southern blot hybridization, and cloned in a lambda-phage vector. The site of transcription termination and the processed 3'-end of 28S RNA were determined on the cloned fragment and the surrounding nucleotide sequence determined. The 3'-terminal nucleotides of mouse 28S RNA are similar to those of yeast, Drosophila and Xenopus although the homology was lost drastically beyond the 3'-end of 28S RNA. 45S precursor RNA terminated at 30 nucleotides downstream from the 3'-end of 28S RNA gene. A structure of a dyad symmetry with a loop was found immediately prior to the termination site of 45S RNA. The rDNA termination site thus shares some common features with termination sites recognized by other RNA polymerases. Images PMID:6281727

46 CFR 525.3 - Availability of marine terminal operator schedules.

Code of Federal Regulations, 2013 CFR

2013-10-01

... that is made available to the public shall be available during normal business hours and in electronic... computer (PC) by: (1) Dial-up connection via public switched telephone networks (PSTN); or (2) The Internet... incoming calls, (ii) Smart terminal capability for VT-100 terminal or terminal emulation access, and (iii...

46 CFR 525.3 - Availability of marine terminal operator schedules.

Code of Federal Regulations, 2012 CFR

2012-10-01

... that is made available to the public shall be available during normal business hours and in electronic... computer (PC) by: (1) Dial-up connection via public switched telephone networks (PSTN); or (2) The Internet... incoming calls, (ii) Smart terminal capability for VT-100 terminal or terminal emulation access, and (iii...

46 CFR 525.3 - Availability of marine terminal operator schedules.

Code of Federal Regulations, 2014 CFR

2014-10-01

... that is made available to the public shall be available during normal business hours and in electronic... computer (PC) by: (1) Dial-up connection via public switched telephone networks (PSTN); or (2) The Internet... incoming calls, (ii) Smart terminal capability for VT-100 terminal or terminal emulation access, and (iii...

26 CFR 1.273-1 - Life or terminable interests.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 26 Internal Revenue 3 2011-04-01 2011-04-01 false Life or terminable interests. 1.273-1 Section 1... (CONTINUED) INCOME TAXES (CONTINUED) Items Not Deductible § 1.273-1 Life or terminable interests. Amounts paid as income to the holder of a life or a terminable interest acquired by gift, bequest, or...

26 CFR 301.7507-9 - Termination of immunity.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 26 Internal Revenue 18 2011-04-01 2011-04-01 false Termination of immunity. 301.7507-9 Section 301... Termination of immunity. (a) In general. (1) In the case of a bank within section 7507(a), immunity will end... depositors. Immunity of a bank within section 7507 (b) is terminated, as to segregated assets, whenever...

26 CFR 301.7507-9 - Termination of immunity.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 26 Internal Revenue 18 2010-04-01 2010-04-01 false Termination of immunity. 301.7507-9 Section 301... Termination of immunity. (a) In general. (1) In the case of a bank within section 7507(a), immunity will end... depositors. Immunity of a bank within section 7507 (b) is terminated, as to segregated assets, whenever...

26 CFR 301.7507-9 - Termination of immunity.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 26 Internal Revenue 18 2012-04-01 2012-04-01 false Termination of immunity. 301.7507-9 Section 301... Termination of immunity. (a) In general. (1) In the case of a bank within section 7507(a), immunity will end... depositors. Immunity of a bank within section 7507 (b) is terminated, as to segregated assets, whenever...

26 CFR 301.7507-9 - Termination of immunity.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 26 Internal Revenue 18 2013-04-01 2013-04-01 false Termination of immunity. 301.7507-9 Section 301... Termination of immunity. (a) In general. (1) In the case of a bank within section 7507(a), immunity will end... depositors. Immunity of a bank within section 7507 (b) is terminated, as to segregated assets, whenever...

26 CFR 301.7507-9 - Termination of immunity.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 26 Internal Revenue 18 2014-04-01 2014-04-01 false Termination of immunity. 301.7507-9 Section 301... Termination of immunity. (a) In general. (1) In the case of a bank within section 7507(a), immunity will end... depositors. Immunity of a bank within section 7507 (b) is terminated, as to segregated assets, whenever...

Effects of Experience on Preference between Forced and Free Choice

ERIC Educational Resources Information Center

Ono, Koichi

2004-01-01

Preference between forced choice and free choice in concurrent-chain schedules of reinforcement was investigated in pigeons after exposure to particular combinations of terminal links. In Experiment 1, in which terminal links always ended with reinforcers, one of three pairs of terminal links was arranged as preexposure: (a) both terminal links…

48 CFR 970.4905-1 - Termination for convenience of the government and default.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Termination for convenience of the government and default. 970.4905-1 Section 970.4905-1 Federal Acquisition Regulations... Termination of Contracts 970.4905-1 Termination for convenience of the government and default. (a) The...

24 CFR 85.44 - Termination for convenience.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Termination for convenience. 85.44 Section 85.44 Housing and Urban Development Office of the Secretary, Department of Housing and Urban... § 85.44 Termination for convenience. Except as provided in § 85.43 awards may be terminated in whole or...

43 CFR 12.84 - Termination for convenience.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Termination for convenience. 12.84 Section 12.84 Public Lands: Interior Office of the Secretary of the Interior ADMINISTRATIVE AND AUDIT... Termination for convenience. Except as provided in § 12.83 awards may be terminated in whole or in part only...

42 CFR 421.205 - Termination by the Secretary.

Code of Federal Regulations, 2010 CFR

2010-10-01

... for termination. The Secretary may terminate a contract with a carrier at any time if he or she... performed its function in a manner inconsistent with the effective and efficient administration of the... intent to terminate the contract, the carrier may request a hearing within 20 days after the date on the...

77 FR 2127 - Birmingham Terminal Railway, L.L.C.-Acquisition and Operation Exemption-Birmingham Southern...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-13

... Terminal Railway, L.L.C.--Acquisition and Operation Exemption--Birmingham Southern Railroad Company Birmingham Terminal Railway, L.L.C. (BHRR), a noncarrier, has filed a verified notice of exemption under 49... Exemption--Birmingham Terminal Railway, L.L.C., wherein Watco Holdings, Inc., seeks Board approval to...

20 CFR 632.23 - Termination and corrective action of a CAP and/or Master Plan.

Code of Federal Regulations, 2012 CFR

2012-04-01

... and/or Master Plan. 632.23 Section 632.23 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION... and Modification Procedures § 632.23 Termination and corrective action of a CAP and/or Master Plan. (a) Emergency Termination. The Department may terminate or suspend a CAP designation or Master Plan under...

20 CFR 632.23 - Termination and corrective action of a CAP and/or Master Plan.

Code of Federal Regulations, 2011 CFR

2011-04-01

... and/or Master Plan. 632.23 Section 632.23 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION... and Modification Procedures § 632.23 Termination and corrective action of a CAP and/or Master Plan. (a) Emergency Termination. The Department may terminate or suspend a CAP designation or Master Plan under...

49 CFR 1247.1 - Annual Report of Cars Loaded and Cars Terminated.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 9 2010-10-01 2010-10-01 false Annual Report of Cars Loaded and Cars Terminated... TRANSPORTATION BOARD, DEPARTMENT OF TRANSPORTATION (CONTINUED) ACCOUNTS, RECORDS AND REPORTS REPORT OF CARS LOADED AND CARS TERMINATED § 1247.1 Annual Report of Cars Loaded and Cars Terminated. Beginning with the...

49 CFR 1247.1 - Annual Report of Cars Loaded and Cars Terminated.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 9 2014-10-01 2014-10-01 false Annual Report of Cars Loaded and Cars Terminated... TRANSPORTATION BOARD, DEPARTMENT OF TRANSPORTATION (CONTINUED) ACCOUNTS, RECORDS AND REPORTS REPORT OF CARS LOADED AND CARS TERMINATED § 1247.1 Annual Report of Cars Loaded and Cars Terminated. Beginning with the...

49 CFR 1247.1 - Annual Report of Cars Loaded and Cars Terminated.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 9 2013-10-01 2013-10-01 false Annual Report of Cars Loaded and Cars Terminated... TRANSPORTATION BOARD, DEPARTMENT OF TRANSPORTATION (CONTINUED) ACCOUNTS, RECORDS AND REPORTS REPORT OF CARS LOADED AND CARS TERMINATED § 1247.1 Annual Report of Cars Loaded and Cars Terminated. Beginning with the...

49 CFR 1247.1 - Annual Report of Cars Loaded and Cars Terminated.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 9 2011-10-01 2011-10-01 false Annual Report of Cars Loaded and Cars Terminated... TRANSPORTATION BOARD, DEPARTMENT OF TRANSPORTATION (CONTINUED) ACCOUNTS, RECORDS AND REPORTS REPORT OF CARS LOADED AND CARS TERMINATED § 1247.1 Annual Report of Cars Loaded and Cars Terminated. Beginning with the...

49 CFR 1247.1 - Annual Report of Cars Loaded and Cars Terminated.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 9 2012-10-01 2012-10-01 false Annual Report of Cars Loaded and Cars Terminated... TRANSPORTATION BOARD, DEPARTMENT OF TRANSPORTATION (CONTINUED) ACCOUNTS, RECORDS AND REPORTS REPORT OF CARS LOADED AND CARS TERMINATED § 1247.1 Annual Report of Cars Loaded and Cars Terminated. Beginning with the...

12 CFR 611.1216 - Public availability of documents related to the termination.

Code of Federal Regulations, 2010 CFR

2010-01-01

... termination. 611.1216 Section 611.1216 Banks and Banking FARM CREDIT ADMINISTRATION FARM CREDIT SYSTEM ORGANIZATION Termination of System Institution Status § 611.1216 Public availability of documents related to the termination. (a) We may post on our Web site, or require you to post on your Web site: (1) Results...

A Preliminary Report on the PLATO V Terminal.

ERIC Educational Resources Information Center

Stifle, J. E.

This report is a preliminary description of a prototype of a second generation version of the PLATO IV (Programmed Logic for Automated Teaching Operations) student terminal. Development of a new terminal has been pursued with two objectives: to generate a more economic version of the PLATO IV terminal, and to expand capacities and performance of…

48 CFR 1819.7210 - Agreement terminations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false Agreement terminations... ADMINISTRATION SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS NASA Mentor-Protégé Program 1819.7210 Agreement terminations. (a) Agreements may be terminated for cause or on a voluntary basis by the mentor or the protégé...

49 CFR 639.31 - Early lease termination or modification.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 7 2011-10-01 2011-10-01 false Early lease termination or modification. 639.31... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION CAPITAL LEASES Lease Management § 639.31 Early lease termination or... eliminated by early termination; and (2) The Federal share of the excess, if any, of the present value of...

1. TERMINAL ROOM, INTERIOR, SHOP LEVEL, SHOWING FIRE EXTINGUISHING SYSTEM ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. TERMINAL ROOM, INTERIOR, SHOP LEVEL, SHOWING FIRE EXTINGUISHING SYSTEM PIPES AND VALVES AT LEFT. Looking southeast from entrance to terminal room. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Test Stand 1-A Terminal Room, Test Area 1-120, north end of Jupiter Boulevard, Boron, Kern County, CA

76 FR 2414 - Termination of a Recreation and Public Purposes Classification and Opening Order in Comanche...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-13

... the operation of the public land laws generally. The classification termination and opening order will...] Termination of a Recreation and Public Purposes Classification and Opening Order in Comanche County, OK AGENCY: Bureau of Land Management, Interior. ACTION: Notice. SUMMARY: This order terminates a Bureau of Land...

5 CFR 837.202 - Annuities that terminate on reemployment.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 5 Administrative Personnel 2 2010-01-01 2010-01-01 false Annuities that terminate on reemployment... Annuities that terminate on reemployment. (a) FERS annuitants. (1) The annuity of a FERS annuitant who is a... terminates on reemployment. (2) The annuity of a FERS annuitant who is a former military reserve technician...

42 CFR 418.22 - Certification of terminal illness.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 42 Public Health 3 2013-10-01 2013-10-01 false Certification of terminal illness. 418.22 Section... Certification of terminal illness. (a) Timing of certification—(1) General rule. The hospice must obtain written certification of terminal illness for each of the periods listed in § 418.21, even if a single election...

42 CFR 418.22 - Certification of terminal illness.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 42 Public Health 3 2012-10-01 2012-10-01 false Certification of terminal illness. 418.22 Section... Certification of terminal illness. (a) Timing of certification—(1) General rule. The hospice must obtain written certification of terminal illness for each of the periods listed in § 418.21, even if a single election...

42 CFR 418.22 - Certification of terminal illness.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 42 Public Health 3 2014-10-01 2014-10-01 false Certification of terminal illness. 418.22 Section... Certification of terminal illness. (a) Timing of certification—(1) General rule. The hospice must obtain written certification of terminal illness for each of the periods listed in § 418.21, even if a single election...

42 CFR 418.22 - Certification of terminal illness.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 3 2011-10-01 2011-10-01 false Certification of terminal illness. 418.22 Section... Certification of terminal illness. (a) Timing of certification—(1) General rule. The hospice must obtain written certification of terminal illness for each of the periods listed in § 418.21, even if a single election...

26 CFR 1.273-1 - Life or terminable interests.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 26 Internal Revenue 3 2012-04-01 2012-04-01 false Life or terminable interests. 1.273-1 Section 1... (CONTINUED) INCOME TAXES (CONTINUED) Items Not Deductible § 1.273-1 Life or terminable interests. Amounts paid as income to the holder of a life or a terminable interest acquired by gift, bequest, or...

26 CFR 1.273-1 - Life or terminable interests.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 26 Internal Revenue 3 2013-04-01 2013-04-01 false Life or terminable interests. 1.273-1 Section 1... (CONTINUED) INCOME TAXES (CONTINUED) Items Not Deductible § 1.273-1 Life or terminable interests. Amounts paid as income to the holder of a life or a terminable interest acquired by gift, bequest, or...

26 CFR 1.273-1 - Life or terminable interests.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 26 Internal Revenue 3 2014-04-01 2014-04-01 false Life or terminable interests. 1.273-1 Section 1... (CONTINUED) INCOME TAXES (CONTINUED) Items Not Deductible § 1.273-1 Life or terminable interests. Amounts paid as income to the holder of a life or a terminable interest acquired by gift, bequest, or...

47 CFR 15.115 - TV interface devices, including cable system terminal devices.

Code of Federal Regulations, 2010 CFR

2010-10-01

... output terminal(s) of the device terminated by a resistance equal to the rated output impedance. The... ohms) matching the rated output impedance of the TV interface device, shall not exceed the following... during maximum amplitude peaks across a resistance (R in ohms) matching the rated output impedance of the...

47 CFR 15.115 - TV interface devices, including cable system terminal devices.

Code of Federal Regulations, 2011 CFR

2011-10-01

... output terminal(s) of the device terminated by a resistance equal to the rated output impedance. The... ohms) matching the rated output impedance of the TV interface device, shall not exceed the following... during maximum amplitude peaks across a resistance (R in ohms) matching the rated output impedance of the...

9 CFR 590.161 - Termination of plant approval.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 2 2013-01-01 2013-01-01 false Termination of plant approval. 590.161... Service § 590.161 Termination of plant approval. When inspection service is not performed at any plant for a period of at least 90 days, plant approval shall terminate upon notice by the Administrator...

9 CFR 590.161 - Termination of plant approval.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Termination of plant approval. 590.161... Service § 590.161 Termination of plant approval. When inspection service is not performed at any plant for a period of at least 90 days, plant approval shall terminate upon notice by the Administrator...

9 CFR 590.161 - Termination of plant approval.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 2 2014-01-01 2014-01-01 false Termination of plant approval. 590.161... Service § 590.161 Termination of plant approval. When inspection service is not performed at any plant for a period of at least 90 days, plant approval shall terminate upon notice by the Administrator...

9 CFR 590.161 - Termination of plant approval.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 2 2012-01-01 2012-01-01 false Termination of plant approval. 590.161... Service § 590.161 Termination of plant approval. When inspection service is not performed at any plant for a period of at least 90 days, plant approval shall terminate upon notice by the Administrator...

42 CFR 63.9 - How may NIH terminate awards?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 42 Public Health 1 2014-10-01 2014-10-01 false How may NIH terminate awards? 63.9 Section 63.9 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING TRAINEESHIPS § 63.9 How may NIH terminate awards? The Director may terminate a traineeship at any...

42 CFR 63.9 - How may NIH terminate awards?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 1 2011-10-01 2011-10-01 false How may NIH terminate awards? 63.9 Section 63.9 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING TRAINEESHIPS § 63.9 How may NIH terminate awards? The Director may terminate a traineeship at any...

42 CFR 63.9 - How may NIH terminate awards?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 42 Public Health 1 2012-10-01 2012-10-01 false How may NIH terminate awards? 63.9 Section 63.9 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING TRAINEESHIPS § 63.9 How may NIH terminate awards? The Director may terminate a traineeship at any...

42 CFR 63.9 - How may NIH terminate awards?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 42 Public Health 1 2013-10-01 2013-10-01 false How may NIH terminate awards? 63.9 Section 63.9 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES FELLOWSHIPS, INTERNSHIPS, TRAINING TRAINEESHIPS § 63.9 How may NIH terminate awards? The Director may terminate a traineeship at any...

45 CFR 1210.3-8 - Termination file and Examiner's report.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 45 Public Welfare 4 2012-10-01 2012-10-01 false Termination file and Examiner's report. 1210.3-8 Section 1210.3-8 Public Welfare Regulations Relating to Public Welfare (Continued) CORPORATION FOR NATIONAL AND COMMUNITY SERVICE VISTA TRAINEE DESELECTION AND VOLUNTEER EARLY TERMINATION PROCEDURES VISTA Volunteer Early Termination § 1210.3-8...

77 FR 5055 - Certain Liquid Crystal Display Devices and Products Containing the Same; Determination Not To...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-01

... presiding administrative law judge (``ALJ'') granting a joint motion to terminate the investigation based on... Motion To Terminate Based on Settlement Agreement; Termination of the Investigation AGENCY: U.S... parties filed a joint motion to terminate the investigation based on a settlement agreement. On July 21...

20 CFR 416.1331 - Termination of your disability or blindness payments.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Termination of your disability or blindness payments. 416.1331 Section 416.1331 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Suspensions and Terminations § 416.1331 Termination of your disability or blindness payments. (a)...

34 CFR 682.608 - Termination of a school's lending eligibility.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 4 2011-07-01 2011-07-01 false Termination of a school's lending eligibility. 682.608... Requirements, Standards, and Payments for Participating Schools § 682.608 Termination of a school's lending eligibility. (a) General. The Secretary may terminate a school's eligibility to make loans under this part if...

Redundancy of primary RNA-binding functions of the bacterial transcription terminator Rho.

PubMed

Shashni, Rajesh; Qayyum, M Zuhaib; Vishalini, V; Dey, Debashish; Sen, Ranjan

2014-09-01

The bacterial transcription terminator, Rho, terminates transcription at half of the operons. According to the classical model derived from in vitro assays on a few terminators, Rho is recruited to the transcription elongation complex (EC) by recognizing specific sites (rut) on the nascent RNA. Here, we explored the mode of in vivo recruitment process of Rho. We show that sequence specific recognition of the rut site, in majority of the Rho-dependent terminators, can be compromised to a great extent without seriously affecting the genome-wide termination function as well as the viability of Escherichia coli. These terminators function optimally only through a NusG-assisted recruitment and activation of Rho. Our data also indicate that at these terminators, Rho-EC-bound NusG interaction facilitates the isomerization of Rho into a translocase-competent form by stabilizing the interactions of mRNA with the secondary RNA binding site, thereby overcoming the defects of the primary RNA binding functions. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

The fine structure of the terminal segment of the bovine seminiferous tubule.

PubMed

Wrobel, K H; Sinowatz, F; Mademann, R

1982-01-01

The intratesticular excurrent duct system of the bull is composed of rete testis, tubuli recti, and the terminal segment of the seminiferous tubules. Each terminal segment is surrounded by a vascular plexus and may be subdivided into a transitional region, middle portion, and terminal plug. The modified supporting cells of the middle portion and the terminal plug no longer display the typical Sertoli-Sertoli junctions seen in the transitional region and the seminiferous tubule proper. In the region of the terminal plug a distinct central lumen is generally not observed: spermatozoa and tubular fluid must pass through an intricate system of communicating clefts between the apices of the closely attached modified supporting cells. Vacuoles in the supranuclear region of the cells in the middle portion indicate strong transepithelial fluid transport. In analogy to the epithelium of rete testis and tubuli recti, the supporting cells of the terminal segment are capable of phagocytosing spermatozoa. The vascular plexus investing the terminal segment serves a dual purpose: it is a regulatory device for fluid and sperm transport, as well as an area of increased diapedesis for white blood cells.

Biochemistry of terminal deoxynucleotidyltransferase. Identification and unity of ribo- and deoxyribonucleoside triphosphate binding site in terminal deoxynucleotidyltransferase

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pandey, V.N.; Modak, M.J.

Terminal deoxynucleotidyltransferase is the only DNA polymerase that is strongly inhibited in the presence of ATP. We have labeled calf terminal deoxynucleotidyltransferase with (/sup 32/P)ATP in order to identify its binding site in terminal deoxynucleotidyltransferase. The specificity of ATP cross-linking to terminal deoxynucleotidyltransferase is shown by the competitive inhibition of the overall cross-linking reaction by deoxynucleoside triphosphates, as well as the ATP analogs Ap4A and Ap5A. Tryptic peptide mapping of (/sup 32/P)ATP-labeled enzyme revealed a peptide fraction that contained the majority of cross-linked ATP. The properties, chromatographic characteristics, amino acid composition, and sequence analysis of this peptide fraction were identicalmore » with those found associated with dTTP cross-linked terminal deoxynucleotidyl-transferase peptide. The involvement of the same 2 cysteine residues in the crosslinking of both nucleotides further confirmed the unity of the ATP and dTTP binding domain that contains residues 224-237 in the primary amino acid sequence of calf terminal deoxynucleotidyltransferase.« less

“Having Another Child Would Be a Life or Death Situation for Her”: Understanding Pregnancy Termination Among Couples in Rural Bangladesh

PubMed Central

Hindin, Michelle J.

2008-01-01

Objectives. We conducted a mixed-method study in rural southwestern Bangladesh, a country in which an estimated 730 000 elective pregnancy terminations occur each year, to explore women's and couples’ motivations to terminate pregnancies. Methods. Quantitative data derived from a 1998 cross-sectional survey and a longitudinal demographic surveillance system (1998–2003) were combined with qualitative data gathered through 84 in-depth interviews conducted with 19 couples during 2004–2005. Results. Quantitative results indicated that 11% of couples reported a pregnancy termination in the study period; the rate was highest among couples who reported in 1998 that they wanted no more children (29%). Both wives’ and husbands’ fertility preferences independently and significantly predicted pregnancy termination. Qualitative findings showed that more than half of the participants had attempted to terminate a pregnancy at least once in their lifetime. Conclusions. Our results highlight the importance of collecting data from both partners and the influence of husbands’ fertility preferences on reproductive decisionmaking. The prevalence of reported pregnancy terminations in our population, along with the use of informal methods in termination attempts, highlights the need for continued provision of contraceptives and access to safe and affordable pregnancy termination services in this setting. PMID:18703439

Termination patterns of complex partial seizures: An intracranial EEG study.

PubMed

Afra, Pegah; Jouny, Christopher C; Bergey, Gregory K

2015-11-01

While seizure onset patterns have been the subject of many reports, there have been few studies of seizure termination. In this study we report the incidence of synchronous and asynchronous termination patterns of partial seizures recorded with intracranial arrays. Data were collected from patients with intractable complex partial seizures undergoing presurgical evaluations with intracranial electrodes. Patients with seizures originating from mesial temporal and neocortical regions were grouped into three groups based on patterns of seizure termination: synchronous only (So), asynchronous only (Ao), or mixed (S/A, with both synchronous and asynchronous termination patterns). 88% of the patients in the MT group had seizures with a synchronous pattern of termination exclusively (38%) or mixed (50%). 82% of the NC group had seizures with synchronous pattern of termination exclusively (52%) or mixed (30%). In the NC group, there was a significant difference of the range of seizure durations between So and Ao groups, with Ao exhibiting higher variability. Seizures with synchronous termination had low variability in both groups. Synchronous seizure termination is a common pattern for complex partials seizures of both mesial temporal or neocortical onset. This may reflect stereotyped network behavior or dynamics at the seizure focus. Copyright © 2015 British Epilepsy Association. Published by Elsevier Ltd. All rights reserved.

A multivariate prediction model for Rho-dependent termination of transcription.

PubMed

Nadiras, Cédric; Eveno, Eric; Schwartz, Annie; Figueroa-Bossi, Nara; Boudvillain, Marc

2018-06-21

Bacterial transcription termination proceeds via two main mechanisms triggered either by simple, well-conserved (intrinsic) nucleic acid motifs or by the motor protein Rho. Although bacterial genomes can harbor hundreds of termination signals of either type, only intrinsic terminators are reliably predicted. Computational tools to detect the more complex and diversiform Rho-dependent terminators are lacking. To tackle this issue, we devised a prediction method based on Orthogonal Projections to Latent Structures Discriminant Analysis [OPLS-DA] of a large set of in vitro termination data. Using previously uncharacterized genomic sequences for biochemical evaluation and OPLS-DA, we identified new Rho-dependent signals and quantitative sequence descriptors with significant predictive value. Most relevant descriptors specify features of transcript C>G skewness, secondary structure, and richness in regularly-spaced 5'CC/UC dinucleotides that are consistent with known principles for Rho-RNA interaction. Descriptors collectively warrant OPLS-DA predictions of Rho-dependent termination with a ∼85% success rate. Scanning of the Escherichia coli genome with the OPLS-DA model identifies significantly more termination-competent regions than anticipated from transcriptomics and predicts that regions intrinsically refractory to Rho are primarily located in open reading frames. Altogether, this work delineates features important for Rho activity and describes the first method able to predict Rho-dependent terminators in bacterial genomes.

Role of the terminator hairpin in the biogenesis of functional Hfq-binding sRNAs

PubMed Central

Morita, Teppei; Nishino, Ryo; Aiba, Hiroji

2017-01-01

Rho-independent transcription terminators of the genes encoding bacterial Hfq-binding sRNAs possess a set of seven or more T residues at the 3′ end, as noted in previous studies. Here, we have studied the role of the terminator hairpin in the biogenesis of sRNAs focusing on SgrS and RyhB in Escherichia coli. We constructed variant sRNA genes in which the GC-rich inverted repeat sequences are extended to stabilize the terminator hairpins. We demonstrate that the extension of the hairpin stem leads to generation of heterogeneous transcripts in which the poly(U) tail is shortened. The transcripts with shortened poly(U) tails no longer bind to Hfq and lose the ability to repress the target mRNAs. The shortened transcripts are generated in an in vitro transcription system with purified RNA polymerase, indicating that the generation of shortened transcripts is caused by premature transcription termination. We conclude that the terminator structure of sRNA genes is optimized to generate functional sRNAs. Thus, the Rho-independent terminators of sRNA genes possess two common features: a long T residue stretch that is a prerequisite for generation of functional sRNAs and a moderate strength of hairpin structure that ensures the termination at the seventh or longer position within the consecutive T stretch. The modulation of the termination position at the Rho-independent terminators is critical for biosynthesis of functional sRNAs. PMID:28606943

Structural basis for substrate recognition by the human N-terminal methyltransferase 1

DOE PAGES

Dong, Cheng; Mao, Yunfei; Tempel, Wolfram; ...

2015-11-05

α-N-terminal methylation represents a highly conserved and prevalent post-translational modification, yet its biological function has remained largely speculative. The recent discovery of α-N-terminal methyltransferase 1 (NTMT1) and its physiological substrates propels the elucidation of a general role of α-N-terminal methylation in mediating DNA-binding ability of the modified proteins. The phenotypes, observed from both NTMT1 knockdown in breast cancer cell lines and knockout mouse models, suggest the potential involvement of α-N-terminal methylation in DNA damage response and cancer development. In this study, we report the first crystal structures of human NTMT1 in complex with cofactor S-adenosyl-L-homocysteine (SAH) and six substrate peptides,more » respectively, and reveal that NTMT1 contains two characteristic structural elements (a β hairpin and an N-terminal extension) that contribute to its substrate specificity. Our complex structures, coupled with mutagenesis, binding, and enzymatic studies, also present the key elements involved in locking the consensus substrate motif XPK (X indicates any residue type other than D/E) into the catalytic pocket for α-N-terminal methylation and explain why NTMT1 prefers an XPK sequence motif. We propose a catalytic mechanism for α-N-terminal methylation. Overall, this study gives us the first glimpse of the molecular mechanism of α-N-terminal methylation and potentially contributes to the advent of therapeutic agents for human diseases associated with deregulated α-N-terminal methylation.« less

Distinct compartmentalization of dentin matrix protein 1 fragments in mineralized tissues and cells.

PubMed

Maciejewska, Izabela; Qin, Disheng; Huang, Bingzhen; Sun, Yao; Mues, Gabrielle; Svoboda, Kathy; Bonewald, Lynda; Butler, William T; Feng, Jerry Q; Qin, Chunlin

2009-01-01

Dentin matrix protein 1 (DMP1) has been shown to be critical for the formation of dentin and bone. However, the precise pathway by which DMP1 participates in dentinogenesis and osteogenesis remains to be clarified. DMP1 is present in the extracellular matrix of dentin and bone as processed NH(2)- and COOH-terminal fragments. The NH(2)-terminal fragment occurs as a proteoglycan, whereas the COOH-terminal fragment is highly phosphorylated. The differences in biochemical properties suggest that these fragments may have different tissue and cell distribution in association with distinct functions. In this study, we analyzed the distribution of the NH(2)- and COOH-terminal fragments of DMP1 in tooth, bone, osteocytes as well as MC3T3-E1 and HEK-293 cells. Immunohistochemical analyses were performed using antibodies specific to the NH(2)- or COOH-terminal region of DMP1. Clear differences in the distribution of these fragments were observed. In the teeth and bone, the NH(2)-terminal fragment was primarily located in the nonmineralized predentin and cartilage of the growth plate, while the COOH-terminal fragment accumulated in the mineralized zones. In osteocytes, the NH(2)-terminal fragment appeared more abundant along cell membrane and processes of osteocytes, while the COOH-terminal fragment was often found in the nuclei. This pattern of distribution in cellular compartments was further confirmed by analyses on MC3T3-E1 and HEK-293 cells transfected with a construct containing DMP1 cDNA. In these cell lines, the COOH-terminal fragment accumulated in cell nuclei, while the NH(2)-terminal fragment was in the cytosol. The different distribution of DMP1 fragments indicates that these DMP1 variants must perform distinct functions. Copyright 2008 S. Karger AG, Basel.

The N-terminal region of the dopamine D2 receptor, a rhodopsin-like GPCR, regulates correct integration into the plasma membrane and endocytic routes

PubMed Central

Cho, DI; Min, C; Jung, KS; Cheong, SY; Zheng, M; Cheong, SJ; Oak, MH; Cheong, JH; Lee, BK; Kim, KM

2012-01-01

BACKGROUND AND PURPOSE Functional roles of the N-terminal region of rhodopsin-like GPCR family remain unclear. Using dopamine D2 and D3 receptors as a model system, we probed the roles of the N-terminal region in the signalling, intracellular trafficking of receptor proteins, and explored the critical factors that determine the functionality of the N-terminal region. EXPERIMENTAL APPROACH The N-terminal region of the D2 receptor was gradually shortened or switched with that of the D3 receptor or a non-specific sequence (FLAG), or potential N-terminal glycosylation sites were mutated. Effects of these manipulations on surface expression, internalization, post-endocytic behaviours and signalling were determined. KEY RESULTS Shortening the N-terminal region of the D2 receptor enhanced receptor internalization and impaired surface expression and signalling; ligand binding, desensitization and down-regulation were not affected but their association with a particular microdomain, caveolae, was disrupted. Replacement of critical residues within the N-terminal region with the FLAG epitope failed to restore surface expression but partially restored the altered internalization and signalling. When the N-terminal regions were switched between D2 and D3 receptors, cell surface expression pattern of each receptor was switched. Mutations of potential N-terminal glycosylation sites inhibited surface expression but enhanced internalization of D2 receptors. CONCLUSIONS AND IMPLICATIONS Shortening of N-terminus or mutation of glycosylation sites located within the N-terminus enhanced receptor internalization but impaired the surface expression of D2 receptors. The N-terminal region of the D2 receptor, in a sequence-specific manner, controls the receptor's conformation and integration into the plasma membrane, which determine its subcellular localization, intracellular trafficking and signalling properties. PMID:22117524

Psychiatric comorbidity among terminally ill patients in general practice in the Netherlands: a comparison between patients with cancer and heart failure.

PubMed

Ng, Chong Guan; Dijkstra, Ellen; Smeets, Hugo; Boks, Marco P M; de Wit, Niek J

2013-01-01

It is unclear whether psychiatric disorders are specifically related to the terminal phase of cancer, or independent of the underlying disease. To investigate the rate of psychiatric comorbidity and psychotropic drugs prescription in terminally ill patients in the GP setting, comparing both patients with terminal cancer and heart failure. Retrospective cohort study using the Utrecht General Practitioner Research Network. Equally-sized groups of patients with terminal cancer and heart failure were randomly selected from the database of four general practices over the years 2005-2009. Psychiatric comorbidities were determined using the International Classification for Primary Care (ICPC) codes and psychotropic drugs prescriptions using the Anatomical Therapeutic Chemical (ATC) Classification System codes. A total of 191 terminally ill patients were included in the study (111 with cancer and 80 with heart failure). The mean age for patients with terminal cancer (70.8 years, standard deviation [SD] = 12.8) was 15 years younger than that of patients with heart failure (85.6 years, SD = 9.2). Half of the terminally ill patients (50.3 %) were prescribed psychotropics, but only 13.6% of them had obtained a psychiatric diagnosis. There were no significant differences in prevalence of psychiatric disease and psychotropic drug prescription between patients with terminal cancer and heart failure. The results demonstrate a high use of psychotropic drugs in terminally ill patients, often in the absence of a formal diagnosis of a psychiatric disorder. The absence of differences between patients with cancer and heart failure suggests that psychiatric diagnoses and increased psychotropic prescriptions are primarily related to the terminal stage of the disease and not to the background of cancer or heart failure.

Late-Life Suicide in Terminal Cancer: A Rational Act or Underdiagnosed Depression?

PubMed

Cheung, Gary; Douwes, Gwendolyn; Sundram, Frederick

2017-12-01

Previous studies have reported significantly elevated standardized mortality rates in older people with cancer. Terminally ill people represent a unique group where suicide may be considered as rational. The aims of this study are to compare the sociodemographic and clinical characteristics of older people with and without terminal cancer who died by suicide and analyze the suicide motives of those with terminal cancer to determine whether they represent rational suicide. The New Zealand Coronial Services provided records of all older people (aged 65 years and older) who died by suicide between July 2007 and December 2012. Sociodemographic and clinical data were extracted from the records. Using the characteristics for defining rational suicide, we determined whether the motives in terminal cancer cases represented rational suicide. Of the 214 suicide cases, 23 (10.7%) older people were diagnosed with a terminal cancer. Univariate analysis found that older people with terminal cancer who died by suicide were less likely to have a diagnosis of depression (8.7% vs. 46.6%; P = 0.001) or previous contact with mental health services (4.5% vs. 35.0%; P = 0.004) than those without terminal cancer. About 82.6% of the terminal cancer cases had a motivational basis that would be understandable to uninvolved observers. A high proportion of those with terminal cancer had motives suggestive of rational suicide. Future studies are needed to clarify whether the low rate of depression is secondary to underdiagnosis of depression or people with terminal cancer choosing to end their life as a rational act to alleviate suffering. Copyright © 2017 American Academy of Hospice and Palliative Medicine. Published by Elsevier Inc. All rights reserved.

Terminal hospitalizations of nursing home residents: does facility increasing the rate of do not resuscitate orders reduce them?

PubMed

Teno, Joan M; Gozalo, Pedro; Mitchell, Susan L; Bynum, Julie P W; Dosa, David; Mor, Vincent

2011-06-01

Terminal hospitalizations are costly and often avoidable with appropriate advance care planning. This study examined the association between advance care planning, as measured by facility rate of do not resuscitate (DNR) orders in U.S. nursing homes (NHs) and changes in terminal hospitalization rates. Retrospective cohort study of the changing prevalence of DNR orders in U.S. NHs. Using a fixed effect multivariate model, we examined whether increasing facility rate of DNR orders correlates with reductions in terminal hospitalizations in the last week of life, controlling for changes in facility characteristics (staffing, use of NP/PA, case mix of nursing residents, admission volume, racial composition, payer mix). The average facility rate of terminal hospitalizations was 15.5%, fluctuating between 1999 (15.0%) and 2007 (14.8%). NHs starting with low rates of DNR orders that increased their rates had fewer terminal hospital admissions in 2007 (11.2%) than facilities with continuously low DNR usage. Even after applying a multivariate fixed effect model, the effect of changes in facility DNR order rate on terminal hospitalization was -0.056 (95% confidence interval: -0.061, -0.050), indicating that for every 10% increase in DNR orders there was 0.56% decrease in terminal hospitalizations. This rate can be compared with the increase of 0.70% in the terminal hospitalization rate when an NH became disproportionately dependent on Medicaid funding or the 0.40% decrease in terminal hospitalization rate associated with adding a nurse practitioner to the clinical staff complement. NHs that changed their culture of decision making by increasing their facility rate of DNR orders decreased their rate of terminal hospitalizations. Copyright © 2011 U.S. Cancer Pain Relief Committee. Published by Elsevier Inc. All rights reserved.

Gamma-aminobutyric acid (GABA) and neuropeptides in neural areas mediating motion-induced emesis

NASA Technical Reports Server (NTRS)

Damelio, F.; Daunton, Nancy G.; Fox, Robert A.

1991-01-01

Immunocytochemical methods were employed to localize the neurotransmitter amino acid gamma-aminobutyric acid and the neuropeptides substance P and Met-enkephalin in the area postrema (AP), area subpostrema (ASP), nucleus of the tractus solitarius (NTS), dorsal motor nucleus of the vagus nerve (DMNV), and lateral vestibular nucleus (LVN). Glutamic acid decarboxylase immunoreactive (GAD-IR) terminals and fibers were observed in the AP and particularly in the ASP. A gradual decrease in the density of terminals was seen towards the solitary complex. The DMNV revealed irregularly scattered GAD-IR terminals within the neuropil or closely surrounding neuronal cell bodies. The LVN, particularly the dorsal division, showed numerous axon terminals which were mostly localize around large neurons and their proximal dendrites. Substance P immunoreactive (SP-IR) terminals and fibers showed high density in the solitary complex, in particular within the lateral division. The ASP showed medium to low density of SP-IR fibers and terminals. The AP exhibited a small number of fibers and terminals irregularly distributed. The DMNV revealed a high density of SP-IR terminals and fibers that were mainly concentrated in the periphery. Very few terminals were detected in the LVN. Met-enkephalin immunoreactive (Met-Enk-IR) fibers and terminals showed high density and uniform distribution in the DMNV. Scattered terminals and fibers were observed in the AP, ASP, and NTS (particularly the lateral division). The very few fibers were observed in the LVN surrounded the neuronal cell bodies. The present report is part of a study designed to investigate the interaction between neuropeptides and conventional neurotransmitters under conditions producing motion sickness and in the process of sensory-motor adaptation.

Role of the C-terminal extensions of alpha-crystallins. Swapping the C-terminal extension of alpha-crystallin to alphaB-crystallin results in enhanced chaperone activity.

PubMed

Pasta, Saloni Yatin; Raman, Bakthisaran; Ramakrishna, Tangirala; Rao, Ch Mohan

2002-11-29

Several small heat shock proteins contain a well conserved alpha-crystallin domain, flanked by an N-terminal domain and a C-terminal extension, both of which vary in length and sequence. The structural and functional role of the C-terminal extension of small heat shock proteins, particularly of alphaA- and alphaB-crystallins, is not well understood. We have swapped the C-terminal extensions between alphaA- and alphaB-crystallins and generated two novel chimeric proteins, alphaABc and alphaBAc. We have investigated the domain-swapped chimeras for structural and functional alterations. We have used thermal and non-thermal models of protein aggregation and found that the chimeric alphaB with the C-terminal extension of alphaA-crystallin, alphaBAc, exhibits dramatically enhanced chaperone-like activity. Interestingly, however, the chimeric alphaA with the C-terminal extension of alphaB-crystallin, alphaABc, has almost lost its activity. Pyrene solubilization and bis-1-anilino-8-naphthalenesulfonate binding studies show that alphaBAc exhibits more solvent-exposed hydrophobic pockets than alphaA, alphaB, or alphaABc. Significant tertiary structural changes are revealed by tryptophan fluorescence and near-UV CD studies upon swapping the C-terminal extensions. The far-UV CD spectrum of alphaBAc differs from that of alphaB-crystallin whereas that of alphaABc overlaps with that of alphaA-crystallin. Gel filtration chromatography shows alteration in the size of the proteins upon swapping the C-terminal extensions. Our study demonstrates that the unstructured C-terminal extensions play a crucial role in the structure and chaperone activity, in addition to generally believed electrostatic "solubilizer" function.

Structure and Regulatory Interactions of the Cytoplasmic Terminal Domains of Serotonin Transporter

PubMed Central

2014-01-01

Uptake of neurotransmitters by sodium-coupled monoamine transporters of the NSS family is required for termination of synaptic transmission. Transport is tightly regulated by protein–protein interactions involving the small cytoplasmic segments at the amino- and carboxy-terminal ends of the transporter. Although structures of homologues provide information about the transmembrane regions of these transporters, the structural arrangement of the terminal domains remains largely unknown. Here, we combined molecular modeling, biochemical, and biophysical approaches in an iterative manner to investigate the structure of the 82-residue N-terminal and 30-residue C-terminal domains of human serotonin transporter (SERT). Several secondary structures were predicted in these domains, and structural models were built using the Rosetta fragment-based methodology. One-dimensional 1H nuclear magnetic resonance and circular dichroism spectroscopy supported the presence of helical elements in the isolated SERT N-terminal domain. Moreover, introducing helix-breaking residues within those elements altered the fluorescence resonance energy transfer signal between terminal cyan fluorescent protein and yellow fluorescent protein tags attached to full-length SERT, consistent with the notion that the fold of the terminal domains is relatively well-defined. Full-length models of SERT that are consistent with these and published experimental data were generated. The resultant models predict confined loci for the terminal domains and predict that they move apart during the transport-related conformational cycle, as predicted by structures of homologues and by the “rocking bundle” hypothesis, which is consistent with spectroscopic measurements. The models also suggest the nature of binding to regulatory interaction partners. This study provides a structural context for functional and regulatory mechanisms involving SERT terminal domains. PMID:25093911

Structure of the Tropomyosin Overlap Complex from Chicken Smooth Muscle: Insight into the Diversity of N-Terminal Recognition

DOE Office of Scientific and Technical Information (OSTI.GOV)

Frye, Jeremiah; Klenchin, Vadim A.; Rayment, Ivan

Tropomyosin is a stereotypical {alpha}-helical coiled coil that polymerizes to form a filamentous macromolecular assembly that lies on the surface of F-actin. The interaction between the C-terminal and N-terminal segments on adjacent molecules is known as the overlap region. We report here two X-ray structures of the chicken smooth muscle tropomyosin overlap complex. A novel approach was used to stabilize the C-terminal and N-terminal fragments. Globular domains from both the human DNA ligase binding protein XRCC4 and bacteriophage {phi}29 scaffolding protein Gp7 were fused to 37 and 28 C-terminal amino acid residues of tropomyosin, respectively, whereas the 29 N-terminal aminomore » acids of tropomyosin were fused to the C-terminal helix bundle of microtubule binding protein EB1. The structures of both the XRCC4 and Gp7 fusion proteins complexed with the N-terminal EB1 fusion contain a very similar helix bundle in the overlap region that encompasses {approx}15 residues. The C-terminal coiled coil opens to allow formation of the helix bundle, which is stabilized by hydrophobic interactions. These structures are similar to that observed in the NMR structure of the rat skeletal overlap complex [Greenfield, N. J., et al. (2006) J. Mol. Biol. 364, 80-96]. The interactions between the N- and C-terminal coiled coils of smooth muscle tropomyosin show significant curvature, which differs somewhat between the two structures and implies flexibility in the overlap complex, at least in solution. This is likely an important attribute that allows tropomyosin to assemble around the actin filaments. These structures provide a molecular explanation for the role of N-acetylation in the assembly of native tropomyosin.« less

Non-peptidergic small diameter primary afferents expressing VGluT2 project to lamina I of mouse spinal dorsal horn

PubMed Central

2011-01-01

Background Unmyelinated primary afferent nociceptors are commonly classified into two main functional types: those expressing neuropeptides, and non-peptidergic fibers that bind the lectin IB4. However, many small diameter primary afferent neurons neither contain any known neuropeptides nor bind IB4. Most express high levels of vesicular glutamate transporter 2 (VGluT2) and are assumed to be glutamatergic nociceptors but their terminations within the spinal cord are unknown. We used in vitro anterograde axonal tracing with Neurobiotin to identify the central projections of these putative glutamatergic nociceptors. We also quantitatively characterised the spatial arrangement of these terminals with respect to those that expressed the neuropeptide, calcitonin gene-related peptide (CGRP). Results Neurobiotin-labeled VGluT2-immunoreactive (IR) terminals were restricted to lamina I, with a medial-to-lateral distribution similar to CGRP-IR terminals. Most VGluT2-IR terminals in lateral lamina I were not labeled by Neurobiotin implying that they arose mainly from central neurons. 38 ± 4% of Neurobiotin-labeled VGluT2-IR terminals contained CGRP-IR. Conversely, only 17 ± 4% of Neurobiotin-labeled CGRP-IR terminals expressed detectable VGluT2-IR. Neurobiotin-labeled VGluT2-IR or CGRP-IR terminals often aggregated into small clusters or microdomains partially surrounding intrinsic lamina I neurons. Conclusions The central terminals of primary afferents which express high levels of VGluT2-IR but not CGRP-IR terminate mainly in lamina I. The spatial arrangement of VGluT2-IR and CGRP-IR terminals suggest that lamina I neurons receive convergent inputs from presumptive nociceptors that are primarily glutamatergic or peptidergic. This reveals a previously unrecognized level of organization in lamina I consistent with the presence of multiple nociceptive processing pathways. PMID:22152428

7 CFR 1216.29 - Terminate.

Code of Federal Regulations, 2010 CFR

2010-01-01

... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE PEANUT PROMOTION, RESEARCH, AND INFORMATION ORDER Peanut Promotion, Research, and Information Order Definitions § 1216.29 Terminate. Terminate...

7 CFR 1216.29 - Terminate.

Code of Federal Regulations, 2011 CFR

2011-01-01

... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE PEANUT PROMOTION, RESEARCH, AND INFORMATION ORDER Peanut Promotion, Research, and Information Order Definitions § 1216.29 Terminate. Terminate...

7 CFR 1280.125 - Terminate.

Code of Federal Regulations, 2010 CFR

2010-01-01

... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE LAMB PROMOTION, RESEARCH, AND INFORMATION ORDER Lamb Promotion, Research, and Information Order Definitions § 1280.125 Terminate. Terminate...

Regulation of presynaptic Ca2+, synaptic plasticity and contextual fear conditioning by a N-terminal β-amyloid fragment.

PubMed

Lawrence, James L M; Tong, Mei; Alfulaij, Naghum; Sherrin, Tessi; Contarino, Mark; White, Michael M; Bellinger, Frederick P; Todorovic, Cedomir; Nichols, Robert A

2014-10-22

Soluble β-amyloid has been shown to regulate presynaptic Ca(2+) and synaptic plasticity. In particular, picomolar β-amyloid was found to have an agonist-like action on presynaptic nicotinic receptors and to augment long-term potentiation (LTP) in a manner dependent upon nicotinic receptors. Here, we report that a functional N-terminal domain exists within β-amyloid for its agonist-like activity. This sequence corresponds to a N-terminal fragment generated by the combined action of α- and β-secretases, and resident carboxypeptidase. The N-terminal β-amyloid fragment is present in the brains and CSF of healthy adults as well as in Alzheimer's patients. Unlike full-length β-amyloid, the N-terminal β-amyloid fragment is monomeric and nontoxic. In Ca(2+) imaging studies using a model reconstituted rodent neuroblastoma cell line and isolated mouse nerve terminals, the N-terminal β-amyloid fragment proved to be highly potent and more effective than full-length β-amyloid in its agonist-like action on nicotinic receptors. In addition, the N-terminal β-amyloid fragment augmented theta burst-induced post-tetanic potentiation and LTP in mouse hippocampal slices. The N-terminal fragment also rescued LTP inhibited by elevated levels of full-length β-amyloid. Contextual fear conditioning was also strongly augmented following bilateral injection of N-terminal β-amyloid fragment into the dorsal hippocampi of intact mice. The fragment-induced augmentation of fear conditioning was attenuated by coadministration of nicotinic antagonist. The activity of the N-terminal β-amyloid fragment appears to reside largely in a sequence surrounding a putative metal binding site, YEVHHQ. These findings suggest that the N-terminal β-amyloid fragment may serve as a potent and effective endogenous neuromodulator. Copyright © 2014 the authors 0270-6474/14/3414210-09$15.00/0.

Flat battery

DOE Office of Scientific and Technical Information (OSTI.GOV)

Waki, E.; Kobayashi, S.; Hashimoto, Y.

A flat battery is described comprising: an electrically insulative sheathing film including a first film portion and a second film portion on opposite sides of a fold line, the film having an outer surface and an inner surface opposite the outer surface, on both of the first and the second film portions. The fold line divides the inner surface into a first inner surface portion on the first film portion and a second inner surface portion on the second film portion, the film being folded along the fold line so that the first inner surface portion faces the second innermore » surface portion. The first and second film portions are sealed to one another along the entire peripheries thereof except along the fold line, the first film portion having first a first terminal hole and a second terminal hole formed therein; a first collector formed in a plane on the first inner surface portion and having a first terminal portion covering the first terminal hole, the first terminal portion being exposed to the exterior of the battery through the first terminal hole so as to define a first terminal; and a second collector consisting of a first part formed on the first inner surface portion and a second part continuous to the first part formed on the second inner surface portion. The second collector extends across and is folded along the fold line, the second part having a second terminal portion covering the second terminal hole, the second terminal portion being exposed to the exterior of the battery through the second terminal hole so as to define a second terminal. The second part is formed in the plane in spaced non-overlapping relation to the first collector, one of the first and second collectors consisting of a positive collector, the other of the first and second collectors consisting of a negative collector.« less

The investigation of Ag/ZnO interface system by first principle: The structural, electronic and optical properties

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cheng, Hai-Xia; Wang, Xiao-Xu; Beijing Computing Center, Beijing 100094

Ag/ZnO interfaces have been investigated for both of Zn-termination and O-termination by the first principle based on density functional theory. Our calculations demonstrate that the Ag atoms go inward from the Ag/ZnO interface, and the Zn and O atoms are all move outward bulk in the Zn-termination interface, and the changes are just opposite for O-termination. These behaviors are in agreement with the other studies in literatures. Furthermore, an expansion situation is observed in the first two Zn-O bilayer and first three Ag monolayers for both of Zn-termination and O-termination interfaces by comparing with the pure ZnO(0001) and Ag(111) surfaces.more » Moreover, the valence-band both of O-2p and Zn-3d states of Ag/ZnO interface gradual close to Femi level as the Zn, O atoms locate at the deeper layer for Zn-termination, but it is the other way round for O-termination. Calculated absorption spectrum indicates that the absorption intensity of Zn-termination interface is stronger than that of O-termination in the lower energy range (visible light region). These properties of ZnO surfaces are also evaluated for comparison with interfaces. - Graphical abstract: The structures of Ag/ZnO interface: Zn-termination (left) and O-termination (right). In this Ag/ZnO interface system, the ZnO (0001) surface is rotated 30°(R30), and Ag (111) surface is built (2×2) supercell, then a (2×√3) R30 Ag/ZnO interface is constructed using the supercell method (i.e. periodically repeated slabs). The lattice mismatch of (2×√3) R30 Ag/ZnO (2.6% mismatch) is smaller than that of (1×1) Ag/ZnO (11% mismatch).« less

Biochemical Defense Response: Characterizing the Plasticity of Source and Sink in Spring Wheat under Terminal Heat Stress.

PubMed

Kumar, Ranjeet R; Goswami, Suneha; Shamim, Mohammed; Mishra, Upama; Jain, Monika; Singh, Khushboo; Singh, Jyoti P; Dubey, Kavita; Singh, Shweta; Rai, Gyanendra K; Singh, Gyanendra P; Pathak, Himanshu; Chinnusamy, Viswanathan; Praveen, Shelly

2017-01-01

Wheat is highly prone to terminal heat stress (HS) under late-sown conditions. Delayed- sowing is one of the preferred methods to screen the genotypes for thermotolerance under open field conditions. We investigated the effect of terminal HS on the thermotolerance of four popular genotypes of wheat i.e. WR544, HD2967, HD2932, and HD2285 under field condition. We observed significant variations in the biochemical parameters like protein content, antioxidant activity, proline and total reducing sugar content in leaf, stem, and spike under normal (26 ± 2°C) and terminal HS (36 ± 2°C) conditions. Maximum protein, sugars and proline was observed in HD2967, as compared to other cultivars under terminal HS. Wheat cv. HD2967 showed more adaptability to the terminal HS. Differential protein-profiling in leaves, stem and spike of HD2967 under normal (26 ± 2°C) and terminal HS (36 ± 2°C) showed expression of some unique protein spots. MALDI-TOF/MS analysis showed the DEPs as RuBisCO (Rub), RuBisCO activase (Rca), oxygen evolving enhancer protein (OEEP), hypothetical proteins, etc. Expression analysis of genes associated with photosynthesis ( Rub and Rca ) and starch biosynthesis pathway ( AGPase, SSS and SBE ) showed significant variations in the expression under terminal HS. HD2967 showed better performance, as compared to other cultivars under terminal HS. SSS activity observed in HD2967 showed more stability under terminal HS, as compared with other cultivars. Triggering of different biochemical parameters in response to terminal HS was observed to modulate the plasticity of carbon assimilatory pathway. The identified DEPs will enrich the proteomic resources of wheat and will provide a potential biochemical marker for screening wheat germplasm for thermotolerance. The model hypothesized will help the researchers to work in a more focused way to develop terminal heat tolerant wheat without compromising with the quality and quantity of grains.

The synaptic terminations of certain midbrain-olivary fibers in the opossum.

PubMed

King, J S; Hamos, J E; Maley, B E

1978-11-15

The nuclear origin and distribution of midbrain-olivary fibers has been described in a previous study utilizing axonal transport techniques (Linauts and Martin, '78a). The present report extends their results to the electron microscopic level and details the postsynaptic distribution of such fibers. Lesions within the ventral periaqueductal grey and adjacent tegmentum, the red nucleus or the nucleus subparafascicularis result in electron dense axon terminals within the olive at survival times of 48, 72 and 96 hours. At 72 hours, many degenerating presynaptic profiles shrink, become irregular in shape and are totally or partially surrounded by glial processes. The principal olivary nucleus contains the majority of these profiles. However, the subparafascicular terminals are more abundant in the rostral and intermediate parts of the medial accessory nucleus and the rubral terminals are concentrated within the dorsal lamella of the principal nucleus. The nuclear location of the degenerating terminals was determined by examination of 1 micrometer plastic sections cut in the transverse plane from each block face prior to thin sectioning. Degenerating terminals were counted in three cases, one from each of the three lesion sites described above. When taken together these cases show that just over 50% of the degenerating terminals are presynaptic to spiny appendages and are located within the synaptic clusters (glomeruli) described previously (King, '76). The percentage of degenerating terminals in the glomeruli increases to 70% when the lesion is in the ventral periaqueductal grey and adjacent tegmentum. The remaining degenerating terminals contact dendritic shafts outside the astrocytic boundaries of the synaptic clusters. The synpatic vesicle populations within the degenerating terminals vary with the location of the lesion. Lesions in the ventral periaqueductal grey and the adjacent tegmentum result in the degeneration of terminals with either clear spherical vesicles or endings with both clear spherical vesicles and a variable number of large dense core vesicles. In contrast, the primary degenerative changes that occur after destruction of the red nucleus or the nucleus subparafascicularis are in terminals with clear spherical vesicles. When the synaptic complex was present in the plane of section, regardless of the site of the lesion, the degenerating terminals could be classified as Gray's type I. Thus, we have demonstrated that afferents from the mesencephalon terminate within synpatic clusters located in the principal and medial accessory (part A) subnuclei of the inferior olive. Although the mesencephalic afferents have multiple origins (Linauts and Martin, '78a), many of their synaptic terminals contact spiny appendages within the synaptic clusters. This postsynaptic site also receives cerebellar terminals (King et al., '76). The origin of presynaptic profiles within the synaptic clusters that contain clear pleomorphlic vesicles is yet to be determined.

78 FR 48044 - Safety Zone; San Diego International Airport Terminal Two West Grand Opening Fireworks; San Diego...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-07

...-AA00 Safety Zone; San Diego International Airport Terminal Two West Grand Opening Fireworks; San Diego... Opening of Lindbergh Airport Terminal Two West on August 8, 2013. This temporary safety zone is necessary... Diego International Airport Terminal Two grand opening. This safety zone is necessary to provide for the...

21 CFR 56.113 - Suspension or termination of IRB approval of research.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Suspension or termination of IRB approval of research. 56.113 Section 56.113 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN... termination of IRB approval of research. An IRB shall have authority to suspend or terminate approval of...

26 CFR 1.273-1 - Life or terminable interests.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 26 Internal Revenue 3 2010-04-01 2010-04-01 false Life or terminable interests. 1.273-1 Section 1... (CONTINUED) INCOME TAXES Items Not Deductible § 1.273-1 Life or terminable interests. Amounts paid as income to the holder of a life or a terminable interest acquired by gift, bequest, or inheritance shall not...

10 CFR 431.96 - Uniform test method for the measurement of energy efficiency of small, large, and very large...

Code of Federal Regulations, 2011 CFR

2011-01-01

..., packaged terminal air conditioners, and packaged terminal heat pumps. 431.96 Section 431.96 Energy... EQUIPMENT Commercial Air Conditioners and Heat Pumps Test Procedures § 431.96 Uniform test method for the... heating equipment, packaged terminal air conditioners, and packaged terminal heat pumps. (a) Scope. This...

Neural nets with terminal chaos for simulation of non-deterministic patterns

NASA Technical Reports Server (NTRS)

Zak, Michail

1993-01-01

Models for simulating some aspects of neural intelligence are presented and discussed. Special attention is given to terminal neurodynamics as a particular architecture of terminal dynamics suitable for modeling information flows. Applications of terminal chaos to information fusion as well as to planning and modeling coordination among neurons in biological systems are disussed.

26 CFR 36.3121(l)(5)-1 - Effect of termination.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 26 Internal Revenue 15 2010-04-01 2010-04-01 false Effect of termination. 36.3121(l)(5)-1 Section 36.3121(l)(5)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED... SUBSIDIARIES § 36.3121(l)(5)-1 Effect of termination. (a) Termination of entire agreement. (1) If the effective...

42 CFR 423.509 - Termination of contract by CMS.

Code of Federal Regulations, 2010 CFR

2010-10-01

...: (1) Termination of contract by CMS. (i) CMS notifies the Part D plan in writing 90 days before the... difficulties so severe that its ability to make necessary health services available is impaired to the point of... writing that its contract will be terminated on a date specified by CMS. If a termination in is effective...

42 CFR 423.509 - Termination of contract by CMS.

Code of Federal Regulations, 2011 CFR

2011-10-01

...: (1) Termination of contract by CMS. (i) CMS notifies the Part D plan in writing 90 days before the... difficulties so severe that its ability to make necessary health services available is impaired to the point of... writing that its contract will be terminated on a date specified by CMS. If a termination in is effective...

40 CFR 60.502 - Standard for Volatile Organic Compound (VOC) emissions from bulk gasoline terminals.

Code of Federal Regulations, 2014 CFR

2014-07-01

... (VOC) emissions from bulk gasoline terminals. 60.502 Section 60.502 Protection of Environment... SOURCES Standards of Performance for Bulk Gasoline Terminals § 60.502 Standard for Volatile Organic Compound (VOC) emissions from bulk gasoline terminals. On and after the date on which § 60.8(a) requires a...

40 CFR 60.502 - Standard for Volatile Organic Compound (VOC) emissions from bulk gasoline terminals.

Code of Federal Regulations, 2012 CFR

2012-07-01

... (VOC) emissions from bulk gasoline terminals. 60.502 Section 60.502 Protection of Environment... SOURCES Standards of Performance for Bulk Gasoline Terminals § 60.502 Standard for Volatile Organic Compound (VOC) emissions from bulk gasoline terminals. On and after the date on which § 60.8(a) requires a...

40 CFR 60.502 - Standard for Volatile Organic Compound (VOC) emissions from bulk gasoline terminals.

Code of Federal Regulations, 2013 CFR

2013-07-01

... (VOC) emissions from bulk gasoline terminals. 60.502 Section 60.502 Protection of Environment... SOURCES Standards of Performance for Bulk Gasoline Terminals § 60.502 Standard for Volatile Organic Compound (VOC) emissions from bulk gasoline terminals. On and after the date on which § 60.8(a) requires a...

40 CFR 60.502 - Standard for Volatile Organic Compound (VOC) emissions from bulk gasoline terminals.

Code of Federal Regulations, 2011 CFR

2011-07-01

... (VOC) emissions from bulk gasoline terminals. 60.502 Section 60.502 Protection of Environment... SOURCES Standards of Performance for Bulk Gasoline Terminals § 60.502 Standard for Volatile Organic Compound (VOC) emissions from bulk gasoline terminals. On and after the date on which § 60.8(a) requires a...

40 CFR 60.502 - Standard for Volatile Organic Compound (VOC) emissions from bulk gasoline terminals.

Code of Federal Regulations, 2010 CFR

2010-07-01

... (VOC) emissions from bulk gasoline terminals. 60.502 Section 60.502 Protection of Environment... SOURCES Standards of Performance for Bulk Gasoline Terminals § 60.502 Standard for Volatile Organic Compound (VOC) emissions from bulk gasoline terminals. On and after the date on which § 60.8(a) requires a...

10 CFR 431.96 - Uniform test method for the measurement of energy efficiency of small, large, and very large...

Code of Federal Regulations, 2012 CFR

2012-01-01

..., packaged terminal air conditioners, and packaged terminal heat pumps. 431.96 Section 431.96 Energy... EQUIPMENT Commercial Air Conditioners and Heat Pumps Test Procedures § 431.96 Uniform test method for the... heating equipment, packaged terminal air conditioners, and packaged terminal heat pumps. (a) Scope. This...

76 FR 40935 - Vertical Tandem Lifts in Marine Terminals; Extension of the Office of Management and Budget's...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-12

...] Vertical Tandem Lifts in Marine Terminals; Extension of the Office of Management and Budget's (OMB... Standard on Vertical Tandem Lifts (VTLs) in Marine Terminals (29 CFR part 1917). The collection of... on Vertical Tandem Lifts for Marine Terminals (29 CFR part 1917). OSHA is proposing to increase the...

7 CFR 1c.113 - Suspension or termination of IRB approval of research.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 1 2011-01-01 2011-01-01 false Suspension or termination of IRB approval of research... § 1c.113 Suspension or termination of IRB approval of research. An IRB shall have authority to suspend or terminate approval of research that is not being conducted in accordance with the IRB's...

26 CFR 48.4081-3 - Taxable fuel; taxable events other than removal at the terminal rack.

Code of Federal Regulations, 2010 CFR

2010-04-01

... at the terminal rack. 48.4081-3 Section 48.4081-3 Internal Revenue INTERNAL REVENUE SERVICE...; taxable events other than removal at the terminal rack. (a) Overview. Although tax is imposed when taxable fuel is removed from the terminal at the rack, tax also is imposed in certain other situations...

1. Occident Terminal Elevator and annex, (l)1930/workhouse and annex 1925 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. Occident Terminal Elevator and annex, (l)-1930/workhouse and annex 1925 with train shed Peavey Duluth Terminal Annex on left 1930-workhouse 1908 (white silos). - Occident Terminal Elevator & Storage Annex, South side of second slip, north from outer end of Rice's Point, east of Garfield Avenue, Duluth, St. Louis County, MN

Utilization of NASA Lewis mobile terminals for the Hermes satellite

NASA Technical Reports Server (NTRS)

Edelman, E. A.; Fiala, J. L.; Rizzolla, L.

1977-01-01

The high power of the Hermes satellite enables two-way television and voice communication with small ground terminals. The Portable Earth Terminal (PET) and the Transportable Earth Terminal (TET) were developed and built by NASA-Lewis to provide communications capability to short-term users. The NASA-Lewis mobile terminals are described in terms of vehicles and onboard equipment, as well as operation aspects, including use in the field. The section on demonstrations divides the uses into categories of medicine, education, technology and government. Applications of special interest within each category are briefly described.

Preparation of alpha-cyclodextrin-terminated polyrotaxane consisting of beta-cyclodextrins and pluronic as a building block of a biodegradable network.

PubMed

Ooya, Tooru; Ito, Akihiro; Yui, Nobuhiko

2005-05-23

A beta-CD-based biodegradable polyrotaxane was prepared by capping both terminals of polypseudorotaxane consisting of hydrazide-terminated PEG-block-PPG-block-PEG (Pluronic P-105) and beta-CD-succinates with mono-aldehyde alpha-CDs. By decreasing pH, the fluorescent intensity of TNS was increased with time, indicating cleavage of the terminal hydrazone bonds followed by beta-CD-succinate release. The terminal alpha-CD moieties of the polyrotaxane are useful for self-assembled formation with some guest molecules. [Diagram: see text

Pseudo-Hall Effect in Graphite on Paper Based Four Terminal Devices for Stress Sensing Applications

NASA Astrophysics Data System (ADS)

Qamar, Afzaal; Sarwar, Tuba; Dinh, Toan; Foisal, A. R. M.; Phan, Hoang-Phuong; Viet Dao, Dzung

2017-04-01

A cost effective and easy to fabricate stress sensor based on pseudo-Hall effect in Graphite on Paper (GOP) has been presented in this article. The four terminal devices were developed by pencil drawing with hand on to the paper substrate. The stress was applied to the paper containing four terminal devices with the input current applied at two terminals and the offset voltage observed at other two terminals called pseudo-Hall effect. The GOP stress sensor showed significant response to the applied stress which was smooth and linear. These results showed that the pseudo-Hall effect in GOP based four terminal devices can be used for cost effective, flexible and easy to make stress, strain or force sensors.

Gas tube-switched high voltage DC power converter

DOEpatents

She, Xu; Bray, James William; Sommerer, Timothy John; Chokhawala, Rahul

2018-05-15

A direct current (DC)-DC converter includes a transformer and a gas tube-switched inverter circuit. The transformer includes a primary winding and a secondary winding. The gas tube-switched inverter circuit includes first and second inverter load terminals and first and second inverter input terminals. The first and second inverter load terminals are coupled to the primary winding. The first and second inverter input terminals are couplable to a DC node. The gas tube-switched inverter circuit further includes a plurality of gas tube switches respectively coupled between the first and second inverter load terminals and the first and second inverter input terminals. The plurality of gas tube switches is configured to operate to generate an alternating current (AC) voltage at the primary winding.

The management of diabetes in terminal illness related to cancer.

PubMed

King, E J; Haboubi, H; Evans, D; Baker, I; Bain, S C; Stephens, J W

2012-01-01

The management of diabetes during terminal illness is complex, with lack of agreement and consensus among physicians and multidisciplinary teams. Despite the plethora of guidelines available for the management of diabetes, there exists no agreed, evidence-based strategy for managing diabetes during terminal illness and at the end of life. A number of physiological factors may influence glycaemic control during terminal illness. These include anorexia, cachexia, malabsorption, renal and hepatic failure. Furthermore, controversy exists on the frequency of blood glucose monitoring, the optimum blood glucose range and how to achieve this. We review the factors influencing blood glucose during terminal illness and provide a suggested approach to managing patients with type 1 and type 2 diabetes during the early and late stages of terminal illness.

Deciding Termination for Ancestor Match- Bounded String Rewriting Systems

NASA Technical Reports Server (NTRS)

Geser, Alfons; Hofbauer, Dieter; Waldmann, Johannes

2005-01-01

Termination of a string rewriting system can be characterized by termination on suitable recursively defined languages. This kind of termination criteria has been criticized for its lack of automation. In an earlier paper we have shown how to construct an automated termination criterion if the recursion is aligned with the rewrite relation. We have demonstrated the technique with Dershowitz's forward closure criterion. In this paper we show that a different approach is suitable when the recursion is aligned with the inverse of the rewrite relation. We apply this idea to Kurth's ancestor graphs and obtain ancestor match-bounded string rewriting systems. Termination is shown to be decidable for this class. The resulting method improves upon those based on match-boundedness or inverse match-boundedness.

Role of Supportive Care for Terminal Stage Hepatocellular Carcinoma

PubMed Central

Kumar, Manoj; Panda, Dipanjan

2014-01-01

Patients with end stage or terminal HCC are those presenting with tumors leading to a very poor Performance Status (ECOG 3–4) or Child–Pugh C patients with tumors beyond the transplantation threshold. Among HCC patients, 15–20% present with end stage or terminal stage HCC. Their median survival is less than 3–4 months. The management of end stage or terminal HCC is only symptomatic and no definitive tumor directed treatment is indicated. Patients with end stage or terminal HCC should receive palliative support including management of pain, nutrition and psychological support. In general, they should not be considered for participating in clinical trials. This review focuses on palliative care of terminal stage HCC. PMID:25755605

Role of the terminator hairpin in the biogenesis of functional Hfq-binding sRNAs.

PubMed

Morita, Teppei; Nishino, Ryo; Aiba, Hiroji

2017-09-01

Rho-independent transcription terminators of the genes encoding bacterial Hfq-binding sRNAs possess a set of seven or more T residues at the 3' end, as noted in previous studies. Here, we have studied the role of the terminator hairpin in the biogenesis of sRNAs focusing on SgrS and RyhB in Escherichia coli. We constructed variant sRNA genes in which the GC-rich inverted repeat sequences are extended to stabilize the terminator hairpins. We demonstrate that the extension of the hairpin stem leads to generation of heterogeneous transcripts in which the poly(U) tail is shortened. The transcripts with shortened poly(U) tails no longer bind to Hfq and lose the ability to repress the target mRNAs. The shortened transcripts are generated in an in vitro transcription system with purified RNA polymerase, indicating that the generation of shortened transcripts is caused by premature transcription termination. We conclude that the terminator structure of sRNA genes is optimized to generate functional sRNAs. Thus, the Rho-independent terminators of sRNA genes possess two common features: a long T residue stretch that is a prerequisite for generation of functional sRNAs and a moderate strength of hairpin structure that ensures the termination at the seventh or longer position within the consecutive T stretch. The modulation of the termination position at the Rho-independent terminators is critical for biosynthesis of functional sRNAs. © 2017 Morita et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.

Promoter-proximal rDNA terminator augments initiation by preventing disruption of the stable transcription complex caused by polymerase read-in

DOE Office of Scientific and Technical Information (OSTI.GOV)

Henderson, S.L.; Ryan, K.; Sollner-Webb, B.

1989-02-01

We have examined the mechanism by which transcriptional initiation at the mouse rDNA promoter is augmented by the RNA polymerase I terminator element that resides just upstream of it. Using templates in which terminator elements are instead positioned at the opposite side of the plasmid rather than proximal to the promoter, or conditions where transcription is terminated elsewhere in the plasmid by UV-induced lesions, we show that the terminator's stimulatory effect is not position dependent. Mouse terminator elements therefore do not stimulate via the previously postulated 'read-through enhancement' model in which terminated polymerases are handed off to an adjacent promotermore » in a concerted reaction. The position independence and orientation dependence of the terminator also makes it unlikely that the terminator functions as a promoter element or as an enhancer. Instead, terminators serve to augment initiation by preventing polymerases from reading completely around the plasmid and through the promoter from upstream, an event which we show interferes with subsequent rounds of initiation. Notably, this transcriptional interference arises because polymerase passage across a promoter disrupts the otherwise stable transcription complex, specifically releasing the bound transcription factor D. These liberated D molecules can then bind to other templates and activate their expression. The rDNA transcriptional interference is not due to a steric impediment to the binding of new polymerase molecules, and it does not similarly liberate the initiation-competent polymerase (factor C). These studies have also convincingly demonstrated that multiple rounds of transcription are obtained from rDNA template molecules in vitro.« less

Terminal sedation and euthanasia: a comparison of clinical practices.

PubMed

Rietjens, Judith A C; van Delden, Johannes J M; van der Heide, Agnes; Vrakking, Astrid M; Onwuteaka-Philipsen, Bregje D; van der Maas, Paul J; van der Wal, Gerrit

2006-04-10

An important issue in the debate about terminal sedation is the extent to which it differs from euthanasia. We studied clinical differences and similarities between both practices in the Netherlands. Personal interviews were held with a nationwide stratified sample of 410 physicians (response rate, 85%) about the most recent cases in which they used terminal sedation, defined as administering drugs to keep the patient continuously in deep sedation or coma until death without giving artificial nutrition or hydration (n = 211), or performed euthanasia, defined as administering a lethal drug at the request of a patient with the explicit intention to hasten death (n = 123). We compared characteristics of the patients, the decision-making process, and medical care of both practices. Terminal sedation and euthanasia both mostly concerned patients with cancer. Patients receiving terminal sedation were more often anxious (37%) and confused (24%) than patients receiving euthanasia (15% and 2%, respectively). Euthanasia requests were typically related to loss of dignity and a sense of suffering without improving, whereas requesting terminal sedation was more often related to severe pain. Physicians applying terminal sedation estimated that the patient's life had been shortened by more than 1 week in 27% of cases, compared with 73% in euthanasia cases. Terminal sedation and euthanasia both are often applied to address severe suffering in terminally ill patients. However, terminal sedation is typically used to address severe physical and psychological suffering in dying patients, whereas perceived loss of dignity during the last phase of life is a major problem for patients requesting euthanasia.

Fine structural survey of the intermediate subnucleus of the nucleus tractus solitarii and its glossopharyngeal afferent terminals.

PubMed

Hayakawa, Tetsu; Maeda, Seishi; Tanaka, Koichi; Seki, Makoto

2005-10-01

The intermediate subnucleus of the nucleus tractus solitarii (imNTS) receives somatosensory inputs from the soft palate and pharynx, and projects onto the nucleus ambiguus, thus serving as a relay nucleus for swallowing. The ultrastructure and synaptology of the rat imNTS, and its glossopharyngeal afferent terminals, have been examined with cholera toxin-conjugated horseradish peroxidase (CT-HRP) as an anterograde tracer. The imNTS contained oval or ellipsoid-shaped, small to medium-sized neurons (18.2 x 11.4 microm) with little cytoplasm, few cell organelles and an irregularly shaped nucleus. The cytoplasm often contained one or two nucleolus-like stigmoid bodies. The average number of axosomatic terminals was 1.8 per profile. About 83% of them contained round vesicles and formed asymmetric synaptic contacts (Gray's type I), while about 17% contained pleomorphic vesicles and formed symmetric synaptic contacts (Gray's type II). The neuropil contained small or large axodendritic terminals, and about 92% of them were Gray's type I. When CT-HRP was injected into the nodose ganglion, many labeled terminals were found in the imNTS. All anterogradely labeled terminals contacted dendrites but not somata. The labeled terminals were usually large (2.69+/-0.09 mum) and exclusively of Gray's type I. They often contacted more than two dendrites, were covered with glial processes, and formed synaptic glomeruli. A small unlabeled terminal occasionally made an asymmetric synaptic contact with a large labeled terminal. The large glossopharyngeal afferent terminals and the neurons containing stigmoid bodies characterized the imNTS neurons that received pharyngeal afferents.

Detection of prosecretory mitogen lacritin in nonprimate tears primarily as a C-terminal-like fragment.

PubMed

Laurie, Diane E; Splan, Rebecca K; Green, Kari; Still, Katherine M; McKown, Robert L; Laurie, Gordon W

2012-09-12

Lacritin is a human tear glycoprotein that promotes basal tear protein secretion in cultured rat lacrimal acinar cells and proliferation of subconfluent human corneal epithelial cells. When topically added to rabbit eyes, lacritin promotes basal tearing. Despite these activities on several species, lacritin's presence in nonprimate tears or other tissues has not been explored. Here we probed for lacritin in normal horse tears. Sequences were collected from the Ensembl genomic alignment of human LACRT gene with high-quality draft horse genome (EquCab2.0) and analyzed. Normal horse tears were collected and assayed by Western blotting, ELISA, and mass spectrometry. Newly generated rabbit antibodies, respectively, against N- and C-terminal regions of human lacritin were employed. Identity was 75% and 45%, respectively, at nucleotide and protein levels. Structural features were conserved, including a C-terminal amphipathic α-helix. Anti-C-terminal antibodies strongly detected a ∼13 kDa band in horse tears that was validated by mass spectrometry. In human tears, the same antibody detected uncleaved lacritin (∼24 kDa) strongly and C-terminal fragments of ∼13 and ∼11 kDa weakly. Anti-N-terminal antibodies were slightly reactive with a ∼24 kDa horse antigen and showed no reaction with the anti-C-terminal-reactive ∼13 kDa species. Similar respective levels of horse C-terminal versus N-terminal immunoreactivity were apparent by ELISA. Lacritin is present in horse tears, largely as a C-terminal fragment homologous to the mitogenic and bactericidal region in human lacritin, suggesting potential benefit in corneal wound repair.

Budding yeast telomerase RNA transcription termination is dictated by the Nrd1/Nab3 non-coding RNA termination pathway

PubMed Central

Noël, Jean-François; Larose, Stéphanie; Abou Elela, Sherif; Wellinger, Raymund J.

2012-01-01

The RNA component of budding yeast telomerase (Tlc1) occurs in two forms, a non-polyadenylated form found in functional telomerase and a rare polyadenylated version with unknown function. Previous work suggested that the functional Tlc1 polyA− RNA is processed from the polyA+ form, but the mechanisms regulating its transcription termination and 3′-end formation remained unclear. Here we examined transcription termination of Tlc1 RNA in the sequences 3′ of the TLC1 gene and relate it to telomere maintenance. Strikingly, disruption of all probable or cryptic polyadenylation signals near the 3′-end blocked the accumulation of the previously reported polyA+ RNA without affecting the level, function or specific 3′ nucleotide of the mature polyA− form. A genetic approach analysing TLC1 3′-end sequences revealed that transcription terminates upstream of the polyadenylation sites. Furthermore, the results also demonstrate that the function of this Tlc1 terminator depends on the Nrd1/Nab3 transcription termination pathway. The data thus show that transcription termination of the budding yeast telomerase RNA occurs as that of snRNAs and Tlc1 functions in telomere maintenance are not strictly dependent on a polyadenylated precursor, even if the polyA+ form can serve as intermediate in a redundant termination/maturation pathway. PMID:22379137

Distinguishing Core and Holoenzyme Mechanisms of Transcription Termination by RNA Polymerase III

PubMed Central

Arimbasseri, Aneeshkumar G.

2013-01-01

Transcription termination by RNA polymerase (Pol) III serves multiple purposes; it delimits interference with downstream genes, forms 3′ oligo(U) binding sites for the posttranscriptional processing factor, La protein, and resets the polymerase complex for reinitiation. Although an interplay of several Pol III subunits is known to collectively control these activities, how they affect molecular function of the active center during termination is incompletely understood. We have approached this using immobilized Pol III-nucleic acid scaffolds to examine the two major components of termination, transcription pausing and RNA release. This allowed us to distinguish two mechanisms of termination by isolated Saccharomyces cerevisiae Pol III. A core mechanism can operate in the absence of C53/37 and C11 subunits but requires synthesis of 8 or more 3′ U nucleotides, apparently reflecting inherent sensitivity to an oligo(rU·dA) hybrid that is the termination signal proper. The holoenzyme mechanism requires fewer U nucleotides but uses C53/37 and C11 to slow elongation and prevent terminator arrest. N-terminal truncation of C53 or point mutations that disable the cleavage activity of C11 impair their antiarrest activities. The data are consistent with a model in which C53, C37, and C11 activities are functionally integrated with the active center of Pol III during termination. PMID:23401852

A genetic screen for terminator function in yeast identifies a role for a new functional domain in termination factor Nab3.

PubMed

Loya, Travis J; O'Rourke, Thomas W; Reines, Daniel

2012-08-01

The yeast IMD2 gene encodes an enzyme involved in GTP synthesis. Its expression is controlled by guanine nucleotides through a set of alternate start sites and an intervening transcriptional terminator. In the off state, transcription results in a short non-coding RNA that starts upstream of the gene. Transcription terminates via the Nrd1-Nab3-Sen1 complex and is degraded by the nuclear exosome. Using a sensitive terminator read-through assay, we identified trans-acting Terminator Override (TOV) genes that operate this terminator. Four genes were identified: the RNA polymerase II phosphatase SSU72, the RNA polymerase II binding protein PCF11, the TRAMP subunit TRF4 and the hnRNP-like, NAB3. The TOV phenotype can be explained by the loss of function of these gene products as described in models in which termination and RNA degradation are coupled to the phosphorylation state of RNA polymerase II's repeat domain. The most interesting mutations were those found in NAB3, which led to the finding that the removal of merely three carboxy-terminal amino acids compromised Nab3's function. This region of previously unknown function is distant from the protein's well-known RNA binding and Nrd1 binding domains. Structural homology modeling suggests this Nab3 'tail' forms an α-helical multimerization domain that helps assemble it onto an RNA substrate.