9. ENGINE TEST CELL BUILDING INTERIOR. CELL ACCESS ELEVATOR, CELLS ...
9. ENGINE TEST CELL BUILDING INTERIOR. CELL ACCESS ELEVATOR, CELLS 2 AND 4, BASEMENT LEVEL. LOOKING SOUTHEAST. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
10. ENGINE TEST CELL BUILDING INTERIOR. CELL 4, MOUNTING STAND. ...
10. ENGINE TEST CELL BUILDING INTERIOR. CELL 4, MOUNTING STAND. LOOKING NORTHWEST. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
11. ENGINE TEST CELL BUILDING INTERIOR. CONTROL ROOM FOR CELLS ...
11. ENGINE TEST CELL BUILDING INTERIOR. CONTROL ROOM FOR CELLS 2 AND 4. LOOKING SOUTHEAST. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
13. ENGINE TEST CELL BUILDING INTERIOR. EQUIPMENT ROOM SERVING CELLS ...
13. ENGINE TEST CELL BUILDING INTERIOR. EQUIPMENT ROOM SERVING CELLS 2 AND 4. LOOKING SOUTHEAST. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
12. ENGINE TEST CELL BUILDING INTERIOR. DETAIL OF CONTROL CONSOLE ...
12. ENGINE TEST CELL BUILDING INTERIOR. DETAIL OF CONTROL CONSOLE FOR ENGINE TEST CELL 4. LOOKING NORTH. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
2. REAR ELEVATION OF ENGINE TEST CELL BUILDING. LOOKING SOUTHEAST. ...
2. REAR ELEVATION OF ENGINE TEST CELL BUILDING. LOOKING SOUTHEAST. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
14. ENGINE TEST CELL BUILDING ROOF. VENTILATION AND COOLING TOWERS. ...
14. ENGINE TEST CELL BUILDING ROOF. VENTILATION AND COOLING TOWERS. LOOKING EAST. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
8. ENGINE TEST CELL BUILDING INTERIOR. LONGITUDINAL CENTRAL PASSAGEWAY IN ...
8. ENGINE TEST CELL BUILDING INTERIOR. LONGITUDINAL CENTRAL PASSAGEWAY IN BASEMENT. LOOKING SOUTHWEST. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
5. ENGINE TEST CELL BUILDING INTERIOR. CENTRAL ROOM ON MAIN ...
5. ENGINE TEST CELL BUILDING INTERIOR. CENTRAL ROOM ON MAIN FLOOR. LOOKING NORTHWEST. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
15. HISTORIC PHOTOGRAPH. ENGINE TEST CELL BUILDING FRONT FACADE DATED ...
15. HISTORIC PHOTOGRAPH. ENGINE TEST CELL BUILDING FRONT FACADE DATED CA. 1975. LOOKING NORTH. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
4. FRONT FACADE OF ENGINE TEST CELL BUILDING. DETAIL OF ...
4. FRONT FACADE OF ENGINE TEST CELL BUILDING. DETAIL OF MAIN ENTRY. LOOKING NORTHWEST. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
6. ENGINE TEST CELL BUILDING INTERIOR. CENTRAL OFFICE AREA ON ...
6. ENGINE TEST CELL BUILDING INTERIOR. CENTRAL OFFICE AREA ON BASEMENT LEVEL. LOOKING WEST. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
3. FRONT FACADE OF ENGINE TEST CELL BUILDING. VIEW OF ...
3. FRONT FACADE OF ENGINE TEST CELL BUILDING. VIEW OF NORTHEAST WING. LOOKING WEST. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
HOT CELL BUILDING, TRA632. CONTEXTUAL VIEW ALONG WALLEYE AVENUE, CAMERA ...
HOT CELL BUILDING, TRA-632. CONTEXTUAL VIEW ALONG WALLEYE AVENUE, CAMERA FACING EASTERLY. HOT CELL BUILDING IS AT CENTER LEFT OF VIEW; THE LOW-BAY PROJECTION WITH LADDER IS THE TEST TRAIN ASSEMBLY FACILITY, ADDED IN 1968. MTR BUILDING IS IN LEFT OF VIEW. HIGH-BAY BUILDING AT RIGHT IS THE ENGINEERING TEST REACTOR BUILDING, TRA-642. INL NEGATIVE NO. HD46-32-1. Mike Crane, Photographer, 4/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
7. ENGINE TEST CELL BUILDING INTERIOR. WALL MAP IN CENTRAL ...
7. ENGINE TEST CELL BUILDING INTERIOR. WALL MAP IN CENTRAL BASEMENT OFFICE AREA. LOOKING SOUTHWEST. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
HOT CELL BUILDING, TRA632, INTERIOR. CELL 3, "HEAVY" CELL. CAMERA ...
HOT CELL BUILDING, TRA-632, INTERIOR. CELL 3, "HEAVY" CELL. CAMERA FACES WEST TOWARD BUILDING EXIT. OBSERVATION WINDOW AT LEFT EDGE OF VIEW. INL NEGATIVE NO. HD46-28-4. Mike Crane, Photographer, 2/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
17. Building 202, observation room for test cell, showing panel, ...
17. Building 202, observation room for test cell, showing panel, abort button, phones, and observation window. View looking northwest. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
25. Photocopy of engineering drawing, May, 1941 (original drawing located ...
25. Photocopy of engineering drawing, May, 1941 (original drawing located at Fairchild Air Force Base, Civil Engineering Building, Civil Engineering vault). ENGINE TEST CELL BUILDING. ELEVATIONS, RIGHT AND LEFT PORTIONS, TEST CELLS 5-12. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
24. Photocopy of engineering drawing, May, 1941 (original drawing located ...
24. Photocopy of engineering drawing, May, 1941 (original drawing located at Fairchild Air Force Base, Civil Engineering Building, Civil Engineering vault). ENGINE TEST CELL BUILDING. ELEVATIONS, CENTRAL PORTION AND TEST CELLS 1-4. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
26. Photocopy of engineering drawing, May, 1941 (original drawing located ...
26. Photocopy of engineering drawing, May, 1941 (original drawing located at Fairchild Air Force Base, Civil Engineering Building, Civil Engineering vault). ENGINE TEST CELL BUILDING. CROSS-SECTION, CENTRAL PORTION AND TEST CELLS 1-4. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
49. Historic photo of Building 202 test cell interior, test ...
49. Historic photo of Building 202 test cell interior, test stand A with engineer examining damage to test engine, October 21, 1966. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-66-4064. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
21. Photocopy of engineering drawing, May, 1941 (original drawing located ...
21. Photocopy of engineering drawing, May, 1941 (original drawing located at Fairchild Air Force Base, Civil Engineering Building, Civil Engineering vault). ENGINE TEST CELL BUILDING. SECOND FLOOR PLAN, RIGHT AND LEFT END PORTIONS, TEST CELLS 5-12. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
18. Photocopy of engineering drawing, May, 1941 (original drawing located ...
18. Photocopy of engineering drawing, May, 1941 (original drawing located at Fairchild Air Force Base, Civil Engineering Building, Civil Engineering vault). ENGINE TEST CELL BUILDING. FIRST FLOOR PLAN, CENTRAL PORTION AND TEST CELLS 1-4. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
27. Photocopy of engineering drawing, May, 1941 (original drawing located ...
27. Photocopy of engineering drawing, May, 1941 (original drawing located at Fairchild Air Force Base, Civil Engineering Building, Civil Engineering vault). ENGINE TEST CELL BUILDING. CROSS-SECTION, RIGHT AND LEFT END PORTIONS, TEST CELLS 5-12. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
22. Photocopy of engineering drawing, May, 1941 (original drawing located ...
22. Photocopy of engineering drawing, May, 1941 (original drawing located at Fairchild Air Force Base, Civil Engineering Building, Civil Engineering vault). ENGINE TEST CELL BUILDING. THIRD FLOOR AND ROOF PLAN, CENTRAL PORTION AND TEST CELLS 1-4. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
16. Photocopy of engineering drawing, May, 1941 (original drawing located ...
16. Photocopy of engineering drawing, May, 1941 (original drawing located at Fairchild Air Force Base, Civil Engineering Building, Civil Engineering vault). ENGINE TEST CELL BUILDING. BASEMENT FLOOR PLAN, CENTRAL PORTION AND TEST CELLS 1-4. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
17. Photocopy of engineering drawing, May, 1941 (original drawing located ...
17. Photocopy of engineering drawing, May, 1941 (original drawing located at Fairchild Air Force Base, Civil Engineering Building, Civil Engineering vault). ENGINE TEST CELL BUILDING. BASEMENT FLOOR PLAN, RIGHT AND LEFT END PORTIONS, TEST CELLS 5-12. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
20. Photocopy of engineering drawing, May, 1941 (original drawing located ...
20. Photocopy of engineering drawing, May, 1941 (original drawing located at Fairchild Air Force Base, Civil Engineering Building, Civil Engineering vault). ENGINE TEST CELL BUILDING. SECOND FLOOR PLAN, CENTRAL PORTION AND TEST CELLS 1-4. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
19. Photocopy of engineering drawing, May, 1941 (original drawing located ...
19. Photocopy of engineering drawing, May, 1941 (original drawing located at Fairchild Air Force Base, Civil Engineering Building, Civil Engineering vault). ENGINE TEST CELL BUILDING. FIRST FLOOR PLAN, RIGHT AND LEFT END PORTIONS, TEST CELLS 5-12. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
46. Historic photo of Building 202 test cell interior, detail ...
46. Historic photo of Building 202 test cell interior, detail of test stand A with engine severely damaged during testing, September 7, 1961. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-57837. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
47. Historic photo of Building 202 test cell interior, test ...
47. Historic photo of Building 202 test cell interior, test stand A with technician working on zone injector engine, June 3, 1996. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-66-2396. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
23. Photocopy of engineering drawing, May, 1941 (original drawing located ...
23. Photocopy of engineering drawing, May, 1941 (original drawing located at Fairchild Air Force Base, Civil Engineering Building, Civil Engineering vault). ENGINE TEST CELL BUILDING. THIRD FLOOR AND ROOF PLAN, RIGHT AND LEFT END PORTIONS, TEST CELLS 5-12. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
HOT CELL BUILDING, TRA632. EAST END OF BUILDING. CAMERA FACING ...
HOT CELL BUILDING, TRA-632. EAST END OF BUILDING. CAMERA FACING WEST. TRUCK ENCLOSURE (1986) TO THE LEFT, SMALL ADDITION IN ITS SHADOW IS ENCLOSURE OVER METAL PORT INTO HOT CELL NO. 1 (THE OLDEST HOT CELL). NOTE PERSONNEL LADDER AND PLATFORM AT LOFT LEVEL USED WHEN SERVICING AIR FILTERS AND VENTS OF CELL NO. 1. INL NEGATIVE NO. HD46-32-4. Mike Crane, Photographer, 4/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
44. Historic photo of interior of Building 202 test cell, ...
44. Historic photo of interior of Building 202 test cell, showing rocket engine on test stand and camera set up for filming tests, September 1960. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-54464. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
50. Historic photo of Building 202 test cell interior, closeup ...
50. Historic photo of Building 202 test cell interior, closeup of test stand A, with engineer examining damage to test engine, October 21, 1966. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-66-4063. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
23. Construction view of Building 202 test cell, 1956. On ...
23. Construction view of Building 202 test cell, 1956. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA GRC photo number C-952D-1956. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
42. Historic photo of exterior of Building 202 test cell, ...
42. Historic photo of exterior of Building 202 test cell, January 26, 1960. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-52534. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
51. Historic photo of Building 202 test cell interior, with ...
51. Historic photo of Building 202 test cell interior, with longablative rocket engine mounted on test stand A, May 18, 1967. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-66-4084. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
34. Historic photo of Building 202 test cell with damage ...
34. Historic photo of Building 202 test cell with damage from fire or explosion during rocket engine testing, May 17, 1958. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-47965. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
54. Historic photo of Building 202 test cell interior, with ...
54. Historic photo of Building 202 test cell interior, with engine mounted on test stand A, September 13, 1967. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-67-3274. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
52. Historic photo of Building 202 test cell interior, with ...
52. Historic photo of Building 202 test cell interior, with engine mounted on test stand A, May 18, 1967 On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-67-1740. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
37. Historic photo of Building 202 test cell interior, with ...
37. Historic photo of Building 202 test cell interior, with damage related to hydrogen fire during rocket engine testing, April 25, 1959. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-50473. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
40. Historic photo of Building 202 test cell interior, with ...
40. Historic photo of Building 202 test cell interior, with engineers working on rocket engine mounted on test stand A, June 26, 1959. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-51026. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
41. Historic photo of Building 202 test cell interior, Robert ...
41. Historic photo of Building 202 test cell interior, Robert J. Gardener checking fuel implinging qualities of a twenty-thousand-pound-thrust rocket engine injector. Setting appears to be a platform mounted on top of scrubber tank underneath test cell floor, December 1959. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-52166. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
48. Historic photo of Building 202 test cell interior, test ...
48. Historic photo of Building 202 test cell interior, test stand A with zone injector engine; technician is working on equipment panel in foreground, June 3, 1966. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-66-2397. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
28. Photocopy of engineering drawing, May, 1941 (original drawing located ...
28. Photocopy of engineering drawing, May, 1941 (original drawing located at Fairchild Air Force Base, Civil Engineering Building, Civil Engineering vault). ENGINE TEST CELL BUILDING. AIR COOLED TEST STAND. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
39. Historic photo of Building 202 test cell exterior, showing ...
39. Historic photo of Building 202 test cell exterior, showing fiberglass cladding blown out by hydrogen fire during rocket engine testing, April 27, 1959. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-50472. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
38. Historic photo of Building 202 test cell interior, showing ...
38. Historic photo of Building 202 test cell interior, showing damage to test stand A and rocket engine after failure and explosion of engine, December 12, 1958. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-49376. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
57. Historic photo of interior of test cell at Building ...
57. Historic photo of interior of test cell at Building 202, showing test stand A with engine and D.T. support ring, February 24, 1969. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-69--3187. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
HOT CELL BUILDING, TRA632. CONTEXTUAL AERIAL VIEW OF HOT CELL ...
HOT CELL BUILDING, TRA-632. CONTEXTUAL AERIAL VIEW OF HOT CELL BUILDING, IN VIEW AT LEFT, AS YET WITHOUT ROOF. PLUG STORAGE BUILDING LIES BETWEEN IT AND THE SOUTH SIDE OF THE MTR BUILDING AND ITS WING. NOTE CONCRETE DRIVE BETWEEN ROLL-UP DOOR IN MTR BUILDING AND CHARGING FACE OF PLUG STORAGE. REACTOR SERVICES BUILDING (TRA-635) WILL COVER THIS DRIVE AND BUTT UP TO CHARGING FACE. DOTTED LINE IS ON ORIGINAL NEGATIVE. TRA PARKING LOT IN LEFT CORNER OF THE VIEW. CAMERA FACING NORTHWESTERLY. INL NEGATIVE NO. 8274. Unknown Photographer, 7/2/1953 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
53. Historic photo of Building 202 test cell interior, with ...
53. Historic photo of Building 202 test cell interior, with engine mounted on test stand A, showing surrounding fuel and oxidant delivery systems and instruments, May 18, 1967. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-67-1739. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
45. Historic photo of Building 202 test cell interior, with ...
45. Historic photo of Building 202 test cell interior, with engine mounted on test stand A. Close-up view of a twenty-thousand-pound-thrust engine being tested in relation with combustion oscillation studies, October 12, 1960. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-54595. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
20. Building 202, detail of stand A, rocket test stand ...
20. Building 202, detail of stand A, rocket test stand in test cell. View looking southeast. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
9. Exterior view, Test Cell 7, Systems Integration Laboratory Building ...
9. Exterior view, Test Cell 7, Systems Integration Laboratory Building (T-28), looking southwest. The enclosure discussed in CO-88-B-8 is at the right. - Air Force Plant PJKS, Systems Integration Laboratory, Systems Integration Laboratory Building, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
15. INTERIOR VIEW TO THE NORTHEAST OF EQUIPMENT IN ROOM ...
15. INTERIOR VIEW TO THE NORTHEAST OF EQUIPMENT IN ROOM 4, EQUIPMENT AND GENERATOR ROOM, BUILDING 3113/3113A. - Nevada Test Site, Test Cell A Facility, Test Cell A Building & Addition, Area 25, Jackass Flats, Road F, Mercury, Nye County, NV
14. INTERIOR VIEW TO THE EAST OF EQUIPMENT IN ROOM ...
14. INTERIOR VIEW TO THE EAST OF EQUIPMENT IN ROOM 3, FLOW CONTROL ROOM, BUILDING 3113/3113A. - Nevada Test Site, Test Cell A Facility, Test Cell A Building & Addition, Area 25, Jackass Flats, Road F, Mercury, Nye County, NV
2. EXTERIOR VIEW TO THE SOUTH OF THE PIPING ON ...
2. EXTERIOR VIEW TO THE SOUTH OF THE PIPING ON THE ROOF OF AND NEXT TO THE BUILDING. - Nevada Test Site, Test Cell A Facility, Test Cell A Building & Addition, Area 25, Jackass Flats, Road F, Mercury, Nye County, NV
29. Photocopy of engineering drawing, May, 1941 (original drawing located ...
29. Photocopy of engineering drawing, May, 1941 (original drawing located at Fairchild Air Force Base, Civil Engineering Building, Civil Engineering vault). ENGINE TEST CELL BUILDING. 19508 RENOVATION OF FIRST FLOOR, CENTRAL PORTION. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA
HOT CELL BUILDING, TRA632. WHILE STEEL BEAMS DEFINE FUTURE WALLS ...
HOT CELL BUILDING, TRA-632. WHILE STEEL BEAMS DEFINE FUTURE WALLS OF THE BUILDING, SHEET STEEL DEFINES THE HOT CELL "BOX" ITSELF. THREE OPERATING WINDOWS ON LEFT; ONE VIEWING WINDOW ON RIGHT. TUBES WILL CONTAIN SERVICE AND CONTROL LEADS. SPACE BETWEEN INNER AND OUTER BOX WALLS WILL BE FILLED WITH SHIELDED WINDOWS AND BARETES CONCRETE. CAMERA FACES SOUTHEAST. INL NEGATIVE NO. 7933. Unknown Photographer, ca. 5/1953 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
5. Exterior view, enclosure at walkin entry level between Test ...
5. Exterior view, enclosure at walk-in entry level between Test Cell 6 (right) and Test Cell 7 (left), Systems Integration Laboratory Building (T-28), looking southwest. High pressure gas tank and generator test firings are conducted in the enclosure. - Air Force Plant PJKS, Systems Integration Laboratory, Systems Integration Laboratory Building, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
On-site fuel cell field test support program
NASA Astrophysics Data System (ADS)
Staniunas, J. W.; Merten, G. P.
1982-01-01
In order to assess the impact of grid connection on the potential market for fuel cell service, applications studies were conducted to identify the fuel cell operating modes and corresponding fuel cell sizing criteria which offer the most potential for initial commercial service. The market for grid-connected fuel cell service was quantified using United's market analysis program and computerized building data base. Electric and gas consumption data for 268 buildings was added to our surveyed building data file, bringing the total to 407 buildings. These buildings were analyzed for grid-isolated and grid-connected fuel cell service. The results of the analyses indicated that the nursing home, restaurant and health club building sectors offer significant potential for fuel cell service.
HOT CELL BUILDING, TRA632. FLOOR PLAN OF EXPANSION SHOWS LOCATION ...
HOT CELL BUILDING, TRA-632. FLOOR PLAN OF EXPANSION SHOWS LOCATION OF NEW CELLS, "HEAVY" CELL AT WEST END, "LIGHT" CELLS AT EAST. MOCK-UP AND STORAGE AREAS IN SOUTH HALF OF FLOOR. H.K. FERGUSON 895-MTR-ETR-632-A1, 12/1958. INL INDEX NO. 531-0632-00-279-101924, REV. 4. - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
59. Historic elevation and detail drawing of Building 202 test ...
59. Historic elevation and detail drawing of Building 202 test cell, June 29, 1955. NASA GRC drawing no. CE-101341 (On file at NASA Glenn Research Center). - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
HOT CELL BUILDING, TRA632, INTERIOR. CONTEXTUAL VIEW OF HOT CELL ...
HOT CELL BUILDING, TRA-632, INTERIOR. CONTEXTUAL VIEW OF HOT CELL NO. 2 FROM STAIRWAY ALONG NORTH WALL. OBSERVATION WINDOW ALONG WEST SIDE BENEATH "CELL 2" SIGN. DOORWAY IN LEFT OF VIEW LEADS TO CELL 1 WORK AREA OR TO EXIT OUTDOORS TO NORTH. RADIATION DETECTION MONITOR TO RIGHT OF DOOR. CAMERA FACING SOUTHWEST. INL NEGATIVE NO. HD46-28-3. Mike Crane, Photographer, 2/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
HOT CELL BUILDING, TRA632, INTERIOR. WINDOWED ROOM IS OFFICE; NEXT ...
HOT CELL BUILDING, TRA-632, INTERIOR. WINDOWED ROOM IS OFFICE; NEXT DOOR WAS DARKROOM, AND THIRD DOOR LED TO ANOTHER OFFICE. ALL ARE ALONG NORTH WALL OF BUILDING (ETR EXTENSION OF 1958). CAMERA FACES NORTHEAST. PUMICE BLOCK WALLS. INL NEGATIVE NO. HD46-29-1. Mike Crane, Photographer, 2/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
HOT CELL BUILDING, TRA632, INTERIOR. WRIGHT 3TON HOIST ON EAST ...
HOT CELL BUILDING, TRA-632, INTERIOR. WRIGHT 3-TON HOIST ON EAST SIDE OF CELL 2. SIGN AT LEFT OF VIEW SAYS, "...DO NOT BRING FISSILE MATERIAL INTO AREA WITHOUT APPROVAL." CAMERA FACES NORTHWEST. INL NEGATIVE NO. HD46-29-2. Mike Crane, Photographer, 2/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
1. Exterior view of Systems Integration Laboratory Building (T28), looking ...
1. Exterior view of Systems Integration Laboratory Building (T-28), looking northeast. The taller of the two gantries on the left houses Test Cell 6 (fuel), while the shorter gantry on the right houses Test Cell 7 (oxidizer). This structure serves as the functional center of the Systems Integration Laboratory complex for testing, handling, and storage of the Titan II's hydrazine - and nitrogen tetroxide-based fuel system propellants. - Air Force Plant PJKS, Systems Integration Laboratory, Systems Integration Laboratory Building, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
HOT CELL BUILDING, TRA632. ELEVATIONS FOR SOUTH, NORTH AND WEST ...
HOT CELL BUILDING, TRA-632. ELEVATIONS FOR SOUTH, NORTH AND WEST SIDES OF 1958 EXTENSION. H.K. FERGUSON CO. 895-MTR-ETR-632-A3, 12/1958. INL INDEX NO. 531-0632-00-279-101926, REV. 3. - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
REACTOR SERVICE BUILDING, TRA635, CONTEXTUAL VIEW DURING CONSTRUCTION. CAMERA IS ...
REACTOR SERVICE BUILDING, TRA-635, CONTEXTUAL VIEW DURING CONSTRUCTION. CAMERA IS ATOP MTR BUILDING AND LOOKING SOUTHERLY. FOUNDATION AND DRAINS ARE UNDER CONSTRUCTION. THE BUILDING WILL BUTT AGAINST CHARGING FACE OF PLUG STORAGE BUILDING. HOT CELL BUILDING, TRA-632, IS UNDER CONSTRUCTION AT TOP CENTER OF VIEW. INL NEGATIVE NO. 8518. Unknown Photographer, 8/25/1953 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
58. Historic plan, section, and detail drawing of Building 202 ...
58. Historic plan, section, and detail drawing of Building 202 test cell, June 29, 1955. NASA GRC drawing no. CE-101340 (On file at NASA Glenn Research Center). - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
GENERAL VIEW OF FLIGHT LINE BUILDINGS. FROM LEFT TO RIGHT, ...
GENERAL VIEW OF FLIGHT LINE BUILDINGS. FROM LEFT TO RIGHT, JET ENGINE TEST CELL BUILDING (BUILDING 2820), MAINTENANCE DOCK, FLIGHT SYSTEM (BUILDING 2818)" AND MAINTENANCE DOCK (BUILDING 2793). VIEW TO SOUTHEAST - Plattsburgh Air Force Base, U.S. Route 9, Plattsburgh, Clinton County, NY
HOT CELL BUILDING, TRA632. FIRST FLOOR FOUNDATION PLAN SHOWS SECTIONALIZED ...
HOT CELL BUILDING, TRA-632. FIRST FLOOR FOUNDATION PLAN SHOWS SECTIONALIZED FLOOR LOADINGS AND CONCRETE SLAB THICKNESSES, A TYPICAL FEATURE OF NUCLEAR ARCHITECTURE. IDAHO OPERATIONS OFFICE MTR-632-IDO-2, 11/1952. INL INDEX NO. 531-0632-62-396-110561, REV. 1. - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
11. Exterior view, showing instrumentation and gauge panel at walkin ...
11. Exterior view, showing instrumentation and gauge panel at walk-in entry level (bottom) of Test Cell 7, Systems Integration Laboratory Building (T-28), looking west. Metal stair at left leads to working platform levels surrounding test cell. - Air Force Plant PJKS, Systems Integration Laboratory, Systems Integration Laboratory Building, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
IET. Aerial view of project, 95 percent complete. Camera facing ...
IET. Aerial view of project, 95 percent complete. Camera facing east. Left to right: stack, duct, mobile test cell building (TAN-624), four-rail track, dolly. Retaining wall between mobile test building and shielded control building (TAN-620) just beyond. North of control building are tank building (TAN-627) and fuel-transfer pump building (TAN-625). Guard house at upper right along exclusion fence. Construction vehicles and temporary warehouse in view near guard house. Date: June 6, 1955. INEEL negative no. 55-1462 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID
HOT CELL BUILDING, TRA632, INTERIOR. DETAIL OF HOT CELL NO. ...
HOT CELL BUILDING, TRA-632, INTERIOR. DETAIL OF HOT CELL NO. 2 SHOWS MANIPULATION INSTRUMENTS AND SHIELDED OPERATING WINDOWS. PENETRATIONS FOR OPERATING INSTRUMENTS GO THROUGH SHIELDING ABOVE WINDOWS. CONDUIT FOR UTILITIES AND CONTROLS IS BEHIND METAL CABINET BELOW WINDOWS NEAR FLOOR. CAMERA FACES WEST. WARNING SIGN LIMITS FISSILE MATERIAL TO SPECIFIED NUMBER OF GRAMS OF URANIUM AND PLUTONIUM. INL NEGATIVE NO. HD46-28-2. Mike Crane, Photographer, 2/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
29. Historic view of twentythousandpound rocket test stand with engine ...
29. Historic view of twenty-thousand-pound rocket test stand with engine installation in test cell of Building 202, September 1957. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA GRC photo number C-45870. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
LPT. Low power test control building (TAN641) interior. Camera facing ...
LPT. Low power test control building (TAN-641) interior. Camera facing northeast at what remains of control room console. Cut in wall at right of view shows west wall of northern test cell. INEEL negative no. HD-40-4-4 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID
IET. Movable test cell building (TAN624). Plans, sections, and elevations ...
IET. Movable test cell building (TAN-624). Plans, sections, and elevations show trapezoidal shape of front/rear elevations, vertical sliding door panels, wheels, periscope and camera locations, fixed concrete wall, and relationship to coupling station (TAN-620) and rail track. Ralph M. Parson 902-4-ANP-624-A 329. Date: February 1954. Approved by INEEL Classification Office for public release. INEEL Index code no. 035-0624-00-693-106911 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID
10. INTERIOR VIEW TO THE SOUTHEAST OF INSTRUMENTS AND EQUIPMENT ...
10. INTERIOR VIEW TO THE SOUTHEAST OF INSTRUMENTS AND EQUIPMENT IN ROOM 1. - Nevada Test Site, Test Cell A Facility, Test Cell A Building & Addition, Area 25, Jackass Flats, Road F, Mercury, Nye County, NV
11. INTERIOR VIEW TO THE SOUTH OF INSTRUMENTS AND EQUIPMENT ...
11. INTERIOR VIEW TO THE SOUTH OF INSTRUMENTS AND EQUIPMENT IN ROOM 1. - Nevada Test Site, Test Cell A Facility, Test Cell A Building & Addition, Area 25, Jackass Flats, Road F, Mercury, Nye County, NV
HOT CELL BUILDING, TRA632, INTERIOR. HOT CELL NO. 1 (THE ...
HOT CELL BUILDING, TRA-632, INTERIOR. HOT CELL NO. 1 (THE FIRST BUILT) IN LABORATORY 101. CAMERA FACES SOUTHEAST. SHIELDED OPERATING WINDOWS ARE ON LEFT (NORTH) SIDE. OBSERVATION WINDOW IS AT LEFT OF VIEW (ON WEST SIDE). PLASTIC COVERS SHROUD MASTER/SLAVE MANIPULATORS AT WINDOWS IN LEFT OF VIEW. NOTE MINERAL OIL RESERVOIR ABOVE "CELL 1" SIGN, INDICATING LEVEL OF THE FLUID INSIDE THE THICK WINDOWS. HOT CELL HAS BEVELED CORNER BECAUSE A SQUARED CORNER WOULD HAVE SUPPLIED UNNECESSARY SHIELDING. NOTE PUMICE BLOCK WALL AT LEFT OF VIEW. INL NEGATIVE NO. HD46-28-1. Mike Crane, Photographer, 2/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
LPT. Low power assembly and test building (TAN640). Camera facing ...
LPT. Low power assembly and test building (TAN-640). Camera facing west. Rollup doors to each test cell face east. Concrete walls poured in place. Apparatus at right of view was part of a post-ANP program. INEEL negative no. HD-40-1-1 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID
30. Historic view of twentythousandpound rocket test stand with engine ...
30. Historic view of twenty-thousand-pound rocket test stand with engine installation in test cell of Building 202, looking down from elevated location, September 1957. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA GRC photo number C-45872. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
13. INTERIOR VIEW TO THE WEST OF EQUIPMENT IN ROOM ...
13. INTERIOR VIEW TO THE WEST OF EQUIPMENT IN ROOM 2, RADIATION EFFECTS ROOM. - Nevada Test Site, Test Cell A Facility, Test Cell A Building & Addition, Area 25, Jackass Flats, Road F, Mercury, Nye County, NV
3. EXTERIOR VIEW TO THE SOUTHEAST OF THE NORTH AND ...
3. EXTERIOR VIEW TO THE SOUTHEAST OF THE NORTH AND WEST ELEVATIONS OF THE TEST CELL. - Nevada Test Site, Test Cell C Facility, Building No. 3210, Area 25, Jackass Flats, Road J, Mercury, Nye County, NV
12. Exterior view, showing tank and piping associated with Test ...
12. Exterior view, showing tank and piping associated with Test Cell 7, Systems Integration Laboratory Building (T-28), looking west. - Air Force Plant PJKS, Systems Integration Laboratory, Systems Integration Laboratory Building, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
6. EXTERIOR VIEW TO THE NORTHWEST OF THE SOUTHEAST CORNER ...
6. EXTERIOR VIEW TO THE NORTHWEST OF THE SOUTHEAST CORNER OF THE EAST ELEVATION OF THE TEST CELL. - Nevada Test Site, Test Cell C Facility, Building No. 3210, Area 25, Jackass Flats, Road J, Mercury, Nye County, NV
5. EXTERIOR VIEW TO THE WEST OF THE EAST ELEVATION ...
5. EXTERIOR VIEW TO THE WEST OF THE EAST ELEVATION OF THE TEST CELL, WITH DEWARS IN THE BACKGROUND. - Nevada Test Site, Test Cell C Facility, Building No. 3210, Area 25, Jackass Flats, Road J, Mercury, Nye County, NV
4. EXTERIOR VIEW TO THE NORTH OF THE WESTERN PORTION ...
4. EXTERIOR VIEW TO THE NORTH OF THE WESTERN PORTION OF THE SOUTH ELEVATION OF THE TEST CELL. - Nevada Test Site, Test Cell C Facility, Building No. 3210, Area 25, Jackass Flats, Road J, Mercury, Nye County, NV
Engine Propeller Research Building at the Lewis Flight Propulsion Laboratory
1955-02-21
The Engine Propeller Research Building, referred to as the Prop House, emits steam from its acoustic silencers at the National Advisory Committee for Aeronautics (NACA) Lewis Flight Propulsion Laboratory. In 1942 the Prop House became the first completed test facility at the new NACA laboratory in Cleveland, Ohio. It contained four test cells designed to study large reciprocating engines. After World War II, the facility was modified to study turbojet engines. Two of the test cells were divided into smaller test chambers, resulting in a total of six engine stands. During this period the NACA Lewis Materials and Thermodynamics Division used four of the test cells to investigate jet engines constructed with alloys and other high temperature materials. The researchers operated the engines at higher temperatures to study stress, fatigue, rupture, and thermal shock. The Compressor and Turbine Division utilized another test cell to study a NACA-designed compressor installed on a full-scale engine. This design sought to increase engine thrust by increasing its airflow capacity. The higher stage pressure ratio resulted in a reduction of the number of required compressor stages. The last test cell was used at the time by the Engine Research Division to study the effect of high inlet densities on a jet engine. Within a couple years of this photograph the Prop House was significantly altered again. By 1960 the facility was renamed the Electric Propulsion Research Building to better describe its new role in electric propulsion.
Rand, Thomas G; Chang, Carolyn T; McMullin, David R; Miller, J David
2017-09-01
Most fungi that grow on damp building materials produce low molecular weight compounds, some of which are known to be toxic. In this study, we tested the hypothesis that exposure to some metabolites of fungi common on damp building materials would result in time-, dose-, and compound-specific responses in the production of various chemokines by RAW 264.7 cells. Cell cultures were exposed to a 10 -7 M or 10 -8 M metabolite dose for 2, 4, 8 or 24h. Metabolite concentrations used were based on those that might be expected in alveolar macrophages due to inhalation exposure from living or working in a damp building. Compared to controls, exposure provoked significant time-, dose- and compound-specific responses manifest as differentially elevated secretion of three of nine cytokines tested in culture supernatant of treated cells. The greatest number of cytokines produced in response to the metabolites tested were in andrastin A-treated cells (GM-CSF, TGFβ1, Tnf-α) followed by koninginin A (TGFβ1 and Tnf-α) and phomenone (GM-CSF, TGFβ1). Chaetoglobosin A, chaetomugilin D and walleminone exposures each resulted in significant time-specific production of Tnf-α only. This investigation adds to a body of evidence supporting the role of low molecular weight compounds from damp building materials as pathogen associated molecular patterns (PAMPs). Along with fungal glucan and chitin, these compounds contribute to the non-allergy based respiratory outcomes for people living and working in damp buildings. Copyright © 2017 Elsevier Ltd. All rights reserved.
NASA Astrophysics Data System (ADS)
Barrett, Ronald M.; Barrett, Ronald P.; Barrett, Cassandra M.
2017-09-01
This paper lays out the inspiration, operational principles, analytical modeling and coupon testing of a new class of thermally adaptive building coverings. The fundamental driving concepts for these coverings are derived from various families of thermotropic plant structures. Certain plant cellular structures like those in Mimosa pudica (Sensitive Plant), Rhododendron leaves or Albizia julibrissin (Mimosa Tree), exhibit actuation physiology which depends on changes in cellular turgor pressures to generate motion. This form of cellular action via turgor pressure manipulation is an inspiration for a new field of thermally adaptive building coverings which use various forms of cellular foam to aid or enable actuation much like plant cells are used to move leaves. When exposed to high solar loading, the structures use the inherent actuation capability of pockets of air trapped in closed cell foam as actuators to curve plates upwards and outwards. When cold, these same structures curve back towards the building forming large convex pockets of dead air to insulate the building. This paper describes basic classical laminated plate theory models comparing theory and experiment of such coupons containing closed-cell foam actuators. The study concludes with a global description of the effectiveness of this class of thermally adaptive building coverings.
Facilities | Hydrogen and Fuel Cells | NREL
integration research. Photo of the Hydrogen Infrastructure Testing and Research Facility building, with hydrogen fueling station and fuel cell vehicles. Hydrogen Infrastructure Testing and Research Facility The Hydrogen Infrastructure Testing and Research Facility (HITRF) at the ESIF combines electrolyzers, a
Credit PSR. This view shows the north and west facades ...
Credit PSR. This view shows the north and west facades of the building as seen when looking east southeast (1100). This structure was used to test regenerative fuel cells in 1995 - Jet Propulsion Laboratory Edwards Facility, Weigh & Test Preparation Building, Edwards Air Force Base, Boron, Kern County, CA
Adiabatic quantum-flux-parametron cell library adopting minimalist design
DOE Office of Scientific and Technical Information (OSTI.GOV)
Takeuchi, Naoki, E-mail: takeuchi-naoki-kx@ynu.jp; Yamanashi, Yuki; Yoshikawa, Nobuyuki
We herein build an adiabatic quantum-flux-parametron (AQFP) cell library adopting minimalist design and a symmetric layout. In the proposed minimalist design, every logic cell is designed by arraying four types of building block cells: buffer, NOT, constant, and branch cells. Therefore, minimalist design enables us to effectively build and customize an AQFP cell library. The symmetric layout reduces unwanted parasitic magnetic coupling and ensures a large mutual inductance in an output transformer, which enables very long wiring between logic cells. We design and fabricate several logic circuits using the minimal AQFP cell library so as to test logic cells inmore » the library. Moreover, we experimentally investigate the maximum wiring length between logic cells. Finally, we present an experimental demonstration of an 8-bit carry look-ahead adder designed using the minimal AQFP cell library and demonstrate that the proposed cell library is sufficiently robust to realize large-scale digital circuits.« less
Adiabatic quantum-flux-parametron cell library adopting minimalist design
NASA Astrophysics Data System (ADS)
Takeuchi, Naoki; Yamanashi, Yuki; Yoshikawa, Nobuyuki
2015-05-01
We herein build an adiabatic quantum-flux-parametron (AQFP) cell library adopting minimalist design and a symmetric layout. In the proposed minimalist design, every logic cell is designed by arraying four types of building block cells: buffer, NOT, constant, and branch cells. Therefore, minimalist design enables us to effectively build and customize an AQFP cell library. The symmetric layout reduces unwanted parasitic magnetic coupling and ensures a large mutual inductance in an output transformer, which enables very long wiring between logic cells. We design and fabricate several logic circuits using the minimal AQFP cell library so as to test logic cells in the library. Moreover, we experimentally investigate the maximum wiring length between logic cells. Finally, we present an experimental demonstration of an 8-bit carry look-ahead adder designed using the minimal AQFP cell library and demonstrate that the proposed cell library is sufficiently robust to realize large-scale digital circuits.
2017-06-29
This video shows the Space Launch System liquid hydrogen tank structural qualification test article being moved to Building 110, Cell at NASA's Michoud Assembly Facility in New Orleans. The rocket's liquid hydrogen tank, which is the propellant tank that joins to the engine section of the 212-foot tall core stage, will carry cryogenic liquid hydrogen that propels the rocket. This test article build at Michoud is being prepared for testing at NASA's Marshall Space Flight Center in Huntsville, Alabama. There, it will be subjected to millions of pounds of force during testing to ensure the hardware can withstand the incredible stresses of launch.
LPT. Aerial of low power test facility (TAN640 and 641) ...
LPT. Aerial of low power test facility (TAN-640 and -641) and shield test facility (TAN-645 and -646). Camera facing south. Low power reactor cells at left, then one-story control building; diagonal fence; shield test control building, then (high-bay) pool room. In foreground are electrical pad, water tanks and guard house. Photographer: Lowin. Date: February 24, 1965. INEEL negative no. 65-987 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID
4. Exterior view of Components Test Laboratory (T27), looking northeast. ...
4. Exterior view of Components Test Laboratory (T-27), looking northeast. The building wing on the left houses Test Cell 8 (oxidizer) and the oxidizer storage pit or vault, and that on the right houses Test Cell 10 (environmental). - Air Force Plant PJKS, Systems Integration Laboratory, Components Test Laboratory, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
5. Exterior view of Components Test Laboratory (T27), looking northwest. ...
5. Exterior view of Components Test Laboratory (T-27), looking northwest. The building wing on the left houses Test Cell 10 (environmental), and that on the right houses Test Cell 9 (fuel) and the fuel storage pit or vault. - Air Force Plant PJKS, Systems Integration Laboratory, Components Test Laboratory, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
Fabrication and test of inorganic/organic separators. [for silver zinc batteries
NASA Technical Reports Server (NTRS)
Smatko, J. S.
1974-01-01
Completion of testing and failure analysis of MDC 40 Ahr silver zinc cells containing largely inorganic separators was accomplished. The results showed that the wet stand and cycle life objectives of the silver zinc cell development program were accomplished. Building, testing and failure analysis of two plate cells employing three optimum separators selected on the basis of extensive screening tests, was performed. The best separator material as a result of these tests was doped calcium zirconate.
Hydrogen Infrastructure Testing and Research Facility Video (Text Version)
grid integration, continuous code improvement, fuel cell vehicle operation, and renewable hydrogen stations. NRELs research on hydrogen safety provides guidance for safe operation, handling, and use of standards and testing fuel cell and hydrogen components for operation and safety. Building on NRELs Wind-to
Performance and Safety Testing of Cylindrical Moli Lithium-Ion Cells
NASA Technical Reports Server (NTRS)
Jeevarajan, Judith A.; Deng, Yi; Rehm, Ray; Tracinski, Walter A.; Bragg, Bobby J.
2002-01-01
The Moli lithium-ion cells were tested under normal and abuse conditions. The cells exhibit only 50% of their original capacity at about -10 C. The optimum charge/discharge rate with the least percentage loss in capacity is C/2 charge and C/4 discharge. The cells did not explode or go into a thermal runaway during venting at very high temperatures. They exhibited good tolerance under the vibration conditions tested and could potentially be used in the build up of large batteries that have high current pulse (up to 3C) applications.
8. Exterior view, showing tank and associated piping adjacent to ...
8. Exterior view, showing tank and associated piping adjacent to Test Cell 6, Systems Integration Laboratory Building (T-28), looking south. - Air Force Plant PJKS, Systems Integration Laboratory, Systems Integration Laboratory Building, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
6. Exterior view of Components Test Laboratory (T27), looking southwest. ...
6. Exterior view of Components Test Laboratory (T-27), looking southwest. The building wing on the left houses Test Cell 9 (fuel), and that on the right houses the equipment room. The corrugated aluminum shed that is taller than the main building in the left foreground houses a citric acid air pollution control room (also known as scrubber room), the interior of which may be seen in CO-88-A-21. - Air Force Plant PJKS, Systems Integration Laboratory, Components Test Laboratory, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
6. Exterior view, showing structural details and instrumentation at the ...
6. Exterior view, showing structural details and instrumentation at the walk-in entry level (bottom) of Test Cell 6, Systems Integration Laboratory Building (T-28), looking southwest. - Air Force Plant PJKS, Systems Integration Laboratory, Systems Integration Laboratory Building, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
7. Exterior view, showing instrumentation and gauge panel at the ...
7. Exterior view, showing instrumentation and gauge panel at the walk-in entry level (bottom) of Test Cell 6, Systems Integration Laboratory Building (T-28), looking west. - Air Force Plant PJKS, Systems Integration Laboratory, Systems Integration Laboratory Building, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
10. Exterior view, showing the structural details and tanks above ...
10. Exterior view, showing the structural details and tanks above at walk-in entry level (bottom) of Test Cell 7, Systems Integration Laboratory Building (T-28), looking west. - Air Force Plant PJKS, Systems Integration Laboratory, Systems Integration Laboratory Building, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
A&M. Hot liquid waste treatment building (TAN616). Contextual view, facing ...
A&M. Hot liquid waste treatment building (TAN-616). Contextual view, facing south. Wall of hot shop (TAN-607) with high bay at left of view. Lower-roofed building at left edge of view is TAN- 633, hot cell annex. Complex at center of view is TAN-616. Tall metal building with gable roof is TAN-615. Photographer: Ron Paarmann. Date: September 22, 1997. INEEL negative no. HD-20-2-2 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID
Engine Research Building’s Central Control Room
1948-07-21
Operators in the Engine Research Building’s Central Control Room at the National Advisory Committee for Aeronautics (NACA) Lewis Flight Propulsion Laboratory. The massive 4.25-acre Engine Research Building contains dozens of test cells, test stands, and altitude chambers. A powerful collection of compressors and exhausters located in the central portion of the basement provided process air and exhaust for these test areas. This system is connected to similar process air systems in the laboratory’s other large test facilities. The Central Control Room coordinates this activity and communicates with the local utilities. This photograph was taken just after a major upgrade to the control room in 1948. The panels on the wall contain rudimentary floor plans of the different Engine Research Building sections with indicator lights and instrumentation for each test cell. The process air equipment included 12 exhausters, four compressors, a refrigeration system, cooling water, and an exhaust system. The operators in the control room kept in contact with engineers running the process air system and those conducting the tests in the test cells. The operators also coordinated with the local power companies to make sure enough electricity was available to operate the powerful compressors and exhausters.
Performance Assessment of Baseline Cells for the High Efficiency Space Power Systems Project
NASA Technical Reports Server (NTRS)
Schneidegger, Brianne T.
2012-01-01
The Enabling Technology Development and Demonstration (ETDD) Program High Efficiency Space Power Systems (HESPS) Project, formerly the Exploration Technology Development Program (ETDP) Energy Storage Project is tasked with developing advanced lithium-ion cells for future NASA Exploration missions. Under this project, components under development via various in-house and contracted efforts are delivered to Saft America for scale-up and integration into cells. Progress toward meeting project goals will be measured by comparing the performance to these cells with cells of a similar format with Saft s state-of-the-art aerospace chemistry. This report discusses the results of testing performed on the first set of baseline cells delivered by Saft to the NASA Glenn Research Center. This build is a cylindrical "DD" geometry with a 10 Ah nameplate capacity. Testing is being performed to establish baseline cell performance at conditions relevant to ETDD HESPS Battery Key Performance Parameter (KPP) goals including various temperatures, rates, and cycle life conditions. Data obtained from these cells will serve as a performance baseline for future cell builds containing optimized ETDD HESPSdeveloped materials. A test plan for these cells was developed to measure cell performance against the high energy cell KPP goals. The goal for cell-level specific energy of the high energy technology is 180 Wh/kg at a C/10 discharge rate and 0 C. The cells should operate for at least 2000 cycles at 100 percent DOD with 80 percent capacity retention. Baseline DD cells delivered 152 Wh/kg at 20 C. This number decreased to 143.9 Wh/kg with a 0 C discharge. This report provides performance data and summarizes results of the testing performed on the DD cells.
Coherent Turbulence Rig in the Engine Research Building
1979-08-21
An engineer examines the Coherent Turbulence Rig in the Engine Research Building at the National Aeronautics and Space Administration (NASA) Lewis Research Center. Coherent turbulence occurs when waves of uniform size and alignment are present in airflow. Researchers at NASA Lewis were interested in determining the relation between the size of the waves and their heat transfer properties. The massive 4.25-acre Engine Research Building contains dozens of test cells, test stands, and altitude chambers. A powerful a collection of compressors and exhausters located in the central portion of the basement provides process air and exhaust for these test areas. This system is connected to similar process air systems in the laboratory’s other large test facilities. The Central Control Room coordinates this activity and communicates with the local utilities.
1. Exterior view of Components Test Laboratory (T27), looking southeast ...
1. Exterior view of Components Test Laboratory (T-27), looking southeast from hill north of structure. The building wing in the right foreground houses Test Cell 8 (oxidizer) and the oxidizer storage pit or vault. Test Cell 10 is located in the center background, Test Cell 9 is at the far left, and the equipment room is in the immediate left foreground. The control room is in the center of the structure and abuts the aforementioned test cell and equipment room wings. This structure served as a facility for testing, handling, and storage of Titan II's hydrazine- and nitrogen teteroxide-based propellant system components for compatability determinations. - Air Force Plant PJKS, Systems Integration Laboratory, Components Test Laboratory, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
DOE Office of Scientific and Technical Information (OSTI.GOV)
Racine, W.C.; Campillo, C.J.
During the site-selection phase of the Onsite Fuel-Cell Field Test, nearly one hundred sites throughout the U.S. were each instrumented with a standard data-acquisition system (DAS) to collect hourly electrical and thermal data for one year. Seventy of those sites are included in the report. Each site's electrical and thermal systems were instrumented including ambient temperature, electrical demands, building gas usage, and other parameters necessary to calculate building thermal loads. Multifamily residential, commercial, and light industrial sites were instrumented. Approximately twenty market sectors were represented including restaurants, hospitals, hotels, apartments, health clubs, nursing homes, and food-processing plants. The primary usemore » of the data was to determine site compatibility for the installation of 40-kW fuel-cell power plants. However, the collected energy data and site-specific information summarized in this comprehensive report may also be useful for other applications such as market characterization and simulation of new or improved energy-utilization equipment in actual sites. This volume covers metal-plating facilities, nurseries, nursing homes, office buildings and other industrial applications.« less
LPT. Elevations of low power test building (TAN640 and 641). ...
LPT. Elevations of low power test building (TAN-640 and -641). West and south elevations show stepped shield wall. South and east elevations show pumice block passageway on south side. Reactor cell walls are concrete. One-story parts are pumice block. Metal rollup doors. Ralph M. Parsons 1229-12 ANP/GE-7-640-A-2. November 1956. Approved by INEEL Classification Office for public release. INEEL index code no. 038-0640-00-693-107275 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID
Arul, P; Smitha, Shetty; Masilamani, Suresh; Akshatha, C
2018-01-01
Cytogenetic damage in exfoliated buccal epithelial cells due to environmental and occupational exposure is often monitored by micronucleus (MN) assay using liquid based cytology (LBC) preparations. This study was performed to evaluate MN in exfoliated buccal epithelial cells of building construction workers using LBC preparations. LBC preparations of exfoliated buccal epithelial cells from 100 subjects [50 building construction workers (cases) and 50 administrative staffs (controls)] was evaluated by May-Grunwald Giemsa, Hematoxylin and Eosin and Papanicolaou stains. Student's t test was used for statistical analysis and a P value of <0.05 was considered as statistically significant. The mean frequencies of MN for cases were significantly higher than controls regardless of staining methods used. There were statistically significant differences between smokers and non-smokers of the controls as well as duration of working exposure (<5 and >5 years) and smokers and non-smokers of cases (P=0.001). However, there were meaningful differences regarding mean frequencies of MN between smokers, non-smokers, those with alcohol consumption or not in cases and controls using various stains (P=0.001). There was an increased risk of cytogenetic damage in building construction workers. However, evaluation of MN of exfoliated buccal epithelial cells in building construction workers serve as a minimally invasive biomarker for cytogenetic damage. LBC preparations can be applied for MN assay as it improves the quality of smears and cell morphology, decreases the confounding factors and reduces false positive results.
Posttest analysis of beta (Na/S) cells from chloride silent power, limited. Final report
DOE Office of Scientific and Technical Information (OSTI.GOV)
Battles, J.E.; Mrazek, F.C.
Researchers have developed a unique methodology for examining sodium/sulfur cells after testing to learn more about their behavior. The new techniques described in this report allow scientists to discern the physical and chemical states of these high-energy cells and to develop hypotheses about degradation mechanisms. This information may provide a basis for building cells with longer lives.
Nickel-hydrogen cell low-Earth life test update
NASA Technical Reports Server (NTRS)
Frate, David T.
1991-01-01
When individual pressure vessel (IPV) nickel-hydrogen (Ni/H2) cells were selected as the energy storage system for the Space Station Freedom in March of 1986, a limited database existed on life and performance characteristics of these cells in a low earth orbit (LEO) regime. Therefore, NASA LeRC initiated a Ni/H2 cell test program with the primary objectives of building a test facility, procuring cells from existing NASA contracts, and screening several cell designs by life testing in a LEO 35 percent depth of discharge (DOD) scenario. A total of 40 cells incorporating 13 designs were purchased from Yardney, Hughes, and Eagle-Picher. Thirty-two of the cells purchased were 65 A-hr nameplate capacity and eight cells were 50 A-hr. Yardney and Eagle-Picher cells were built with both the Air Force recirculating and the advanced back-to-back electrode stack configurations and incorporated 31 and 26 percent KOH. Acceptance testing of the first delivered cells began in March of 1988, with life testing following in September of that year.Performance comparisons of these cells are made here while specifically addressing life test data relative to the design differences.
Nickel-hydrogen cell low-earth-orbit life test update
NASA Technical Reports Server (NTRS)
Frate, David T.
1991-01-01
When individual pressure vessel (IPV) nickel-hydrogen (Ni/H2) cells were selected as the energy storage system for Space Station Freedom in March of 1986, a limited database existed on life and performance characteristics of these cells in a low earth orbit (LEO) regime. Therefore, NASA LeRC initiated a Ni/H2 cell test program with the primary objectives of building a test facility, procuring cells from existing NASA contracts, and screening several cell designs by life testing in a LEO 35 percent depth of discharge (DOD) scenario. A total of 40 cells incorporating 13 designs were purchased from Yardney, Hughes, and Eagle-Picher. Thirty-two of the cells purchased were 65 A-hr nameplate capacity and eight cells were 50 A-Hr. Yardney and Eagle-Picher cells were built with both the Air Force recirculating and the advanced back-to-back electrode stack configurations and incorporated 31 and 26 percent KOH. Acceptance testing of the first delivered cells began in March of 1988, with life testing following in September of that year. Performance comparisons of these cells are made here while specifically addressing life test data relative to the design differences.
On-site cell field test support program
NASA Astrophysics Data System (ADS)
Staniunas, J. W.; Merten, G. P.
1982-09-01
Utility sites for data monitoring were reviewed and selected. Each of these sites will be instrumented and its energy requirements monitored and analyzed for one year prior to the selection of 40 Kilowatt fuel cell field test sites. Analyses in support of the selection of sites for instrumentation shows that many building sectors offered considerable market potential. These sectors include nursing home, health club, restaurant, industrial, hotel/motel and apartment.
Central Control Room in the Engine Research Building
1968-11-21
Operators in the Engine Research Building’s Central Control Room at the National Aeronautics and Space Administration (NASA) Lewis Research Center. The massive 4.25-acre Engine Research Building contains dozens of test cells, test stands, and altitude chambers. A powerful a collection of compressors and exhausters located in the central portion of the basement provides process air and exhaust for these test areas. This system is connected to similar process air systems in the laboratory’s other large test facilities. The Central Control Room coordinates this activity and communicates with the local utilities. The panels on the wall contain schematics with indicator lights and instrumentation for the atmospheric exhaust, altitude exhaust, refrigerated air, and process air systems. The process air equipment included twelve exhausters, four compressors, refrigeration system, cooling water, and an exhaust system. The operators in the control room kept in contact with engineers running the process air system and those conducting the tests in the test cells. The operators also coordinated with the local power companies to make sure enough electricity was available to operate the powerful compressors and exhausters.
Development of first generation aerospace NiMH cells
NASA Technical Reports Server (NTRS)
Tinker, Lawrence; Dell, Dan; Wu, Tony; Rampel, Guy
1993-01-01
Gates Aerospace Batteries in conjunction with Gates Energy Products (GEP) has been developing NiMH technology for aerospace use since 1990. GEP undertook the development of NiMH technology for commercial cell applications in 1987. This program focused on wound cell technology for replacement of current NiCd technology. As an off shoot of this program small, wound cells were used to evaluate initial design options for aerospace prismatic cell designs. Early in 1991, the first aerospace prismatic cell designs were built in a 6 Ah cell configuration. These cells were used to initially characterize performance in prismatic configurations and begin early life cycle testing. Soon after the 6 Ah cells were on test, several 22 Ah cells were built to test other options. The results of testing of these cells were used to identify potential problem areas for long lived cells and develop solutions to those problems. Following these two cell builds, a set of 7 Ah cells was built to evaluate improvements to the technology. To date results from these tests are very promising. Cycle lives in excess of 2,200 LEO cycles at 50 percent DoD were achieved with cells continuing on test. Results from these cell tests are discussed and data presented to demonstrate feasibility of this technology for aerospace programs.
Design, Build and Qualification of 28 Volt Lithium-Ion battery
NASA Technical Reports Server (NTRS)
Curzon, D.; Spurrett, R.; Rao, G.
2003-01-01
Contents include the following: 1. GSFC performance specifications requirements. 2. AEA philosophy: battery configuration. 3. AEA battery design: SONY cell, cell screening, 20Ah and 60Ah. 4. Testing: 20Ah battery qualification (environmental); 60Ah battery LEO life cycle. 5. Conclusion.: summary and lessons learned.
Study of Charge Build Up in UCN Storage Cell
NASA Astrophysics Data System (ADS)
Broering, Mark; Abney, Josh; Swank, Christopher; Filippone, Bradley; Yao, Weijun; Korsch, Wolfgang
2017-09-01
The neutron EDM collaboration at the Spallation Neutron Source(ORNL) is using ultra-cold neutrons in superfluid helium to improve the nEDM limit by about two orders of magnitude. These neutrons will be stored in target cells located in a strong, stable electric field. Local radiation will generate charged particles which may build up on the target cell walls reducing field strength over time. The field changes need to be kept below 1%, making it necessary to study this cell charging behavior, determine its effect on the experiment and find ways to mitigate this. In order to study this cell charging effect, a compact test setup was designed. Using this scaled down model, charged particles are generated by a 137Cs source and the electric field is monitored via the electo-optic Kerr effect. Liquid nitrogen has a much stronger response to electric fields than helium, making it an ideal candidate for first tests. Cell charging effects have been observed in liquid nitrogen. These results along with the experimental technique and progress toward a superfluid helium measurement will also be presented. This research is supported by DOE Grants: DE-FG02-99ER41101, DE-AC05-00OR22725.
Controlling cell-free metabolism through physiochemical perturbations.
Karim, Ashty S; Heggestad, Jacob T; Crowe, Samantha A; Jewett, Michael C
2018-01-01
Building biosynthetic pathways and engineering metabolic reactions in cells can be time-consuming due to complexities in cellular metabolism. These complexities often convolute the combinatorial testing of biosynthetic pathway designs needed to define an optimal biosynthetic system. To simplify the optimization of biosynthetic systems, we recently reported a new cell-free framework for pathway construction and testing. In this framework, multiple crude-cell extracts are selectively enriched with individual pathway enzymes, which are then mixed to construct full biosynthetic pathways on the time scale of a day. This rapid approach to building pathways aids in the study of metabolic pathway performance by providing a unique freedom of design to modify and control biological systems for both fundamental and applied biotechnology. The goal of this work was to demonstrate the ability to probe biosynthetic pathway performance in our cell-free framework by perturbing physiochemical conditions, using n-butanol synthesis as a model. We carried out three unique case studies. First, we demonstrated the power of our cell-free approach to maximize biosynthesis yields by mapping physiochemical landscapes using a robotic liquid-handler. This allowed us to determine that NAD and CoA are the most important factors that govern cell-free n-butanol metabolism. Second, we compared metabolic profile differences between two different approaches for building pathways from enriched lysates, heterologous expression and cell-free protein synthesis. We discover that phosphate from PEP utilization, along with other physiochemical reagents, during cell-free protein synthesis-coupled, crude-lysate metabolic system operation inhibits optimal cell-free n-butanol metabolism. Third, we show that non-phosphorylated secondary energy substrates can be used to fuel cell-free protein synthesis and n-butanol biosynthesis. Taken together, our work highlights the ease of using cell-free systems to explore physiochemical perturbations and suggests the need for a more controllable, multi-step, separated cell-free framework for future pathway prototyping and enzyme discovery efforts. Copyright © 2017 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.
2. Exterior view of Components Test Laboratory (T27), looking southeast. ...
2. Exterior view of Components Test Laboratory (T-27), looking southeast. The building wing on the left houses the equipment room and that on the right houses Test Cell 8 (oxidizer) and the oxidizer storage pit or vault. - Air Force Plant PJKS, Systems Integration Laboratory, Components Test Laboratory, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
3. Exterior view of Components Test Laboratory (T27), looking southeast. ...
3. Exterior view of Components Test Laboratory (T-27), looking southeast. The building wing on the left houses the equipment room, and that on the right houses Test Cell 8 (oxidizer) and the oxidizer storage pit or vault. - Air Force Plant PJKS, Systems Integration Laboratory, Components Test Laboratory, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
Köster, Sarah; Weitz, David A; Goldman, Robert D; Aebi, Ueli; Herrmann, Harald
2015-02-01
Intermediate filament proteins form filaments, fibers and networks both in the cytoplasm and the nucleus of metazoan cells. Their general structural building plan accommodates highly varying amino acid sequences to yield extended dimeric α-helical coiled coils of highly conserved design. These 'rod' particles are the basic building blocks of intrinsically flexible, filamentous structures that are able to resist high mechanical stresses, that is, bending and stretching to a considerable degree, both in vitro and in the cell. Biophysical and computer modeling studies are beginning to unfold detailed structural and mechanical insights into these major supramolecular assemblies of cell architecture, not only in the 'test tube' but also in the cellular and tissue context. Copyright © 2015 Elsevier Ltd. All rights reserved.
Kleinberger, Rachelle M; Burke, Nicholas A D; Zhou, Christal; Stöver, Harald D H
2016-01-01
A series of polycations prepared by RAFT copolymerization of N-(3-aminopropyl)methacrylamide hydrochloride (APM) and N-(2-hydroxypropyl)methacrylamide, with molecular weights of 15 and 40 kDa, and APM content of 10-75 mol%, were tested as building blocks for electrostatically assembled hydrogels such as those used for cell encapsulation. Complexation and distribution of these copolymers within anionic calcium alginate gels, as well as cytotoxicity, cell attachment, and cell proliferation on surfaces grafted with the copolymers were found to depend on composition and molecular weight. Copolymers with lower cationic charge density and lower molecular weight showed less cytotoxicity and cell adhesion, and were more mobile within alginate gels. These findings aid in designing improved polyelectrolyte complexes for use as biomaterials.
Credit BG. Looking southeast at Test Stand "D" (Building 4223/E24). ...
Credit BG. Looking southeast at Test Stand "D" (Building 4223/E-24). Left foreground contains six high-pressure nitrogen tanks which supplied nitrogen for operation of propellant valves. Several tanks for other substances have been removed from the base of the tower as part of decontamination and dismantling program. The vertical vacuum test cell can be seen in the tower behind the western sunscreen. At the top of the tower in the northeast corner is the interstage condenser used in the series of vacuum ejectors; at the top of the condenser is one of two Z-stage ejectors used to evacuate the condenser. The hoist beam for lifting/lowering rocket engines can be clearly seen projecting to the west over the pavement. In the distance on the right are Clayton water-tube steam generators from Building 4280/E-81, and the towers for Test Stand "C" and its scrubber-condenser - Jet Propulsion Laboratory Edwards Facility, Test Stand D, Edwards Air Force Base, Boron, Kern County, CA
Rise time and response measurements on a LiSOCl2 cell
NASA Technical Reports Server (NTRS)
Bastien, Caroline; Lecomte, Eric J.
1992-01-01
Dynamic impedance tests were performed on a 180 Ah LiSOCl2 cell in the frame of a short term work contract awarded by Aerospatiale as part of the Hermes Space Plane development work. These tests consisted of rise time and response measurements. The rise time test was performed to show the ability to deliver 4 KW, in the nominal voltage range (75-115 V), within less than 100 microseconds, and after a period at rest of 13 days. The response measurements test consisted of step response and frequency response tests. The frequency response test was performed to characterize the response of the LiSOCl2 cell to a positive or negative load step of 10 A starting from various currents. The test was performed for various depths of discharge and various temperatures. The test results were used to build a mathematical, electrical model of the LiSOCl2 cell which are also presented. The test description, test results, electrical modelization description, and conclusions are presented.
X-33 Injector Ignition Single Cell Test
NASA Technical Reports Server (NTRS)
1997-01-01
The X-33 injector ignition single cell was tested at the Marshall Space Flight Center test stand 116. The X-33 was a sub-scale technology demonstrator prototype of a Reusable Launch Vehicle (RLV) manufactured and named by Lockheed Martin as the Venture Star. The goal of the program was to demonstrate the technologies needed for a full size, single-stage-to-orbit RLV, thus enabling private industry to build and operate the RLV in the first decade of the 21st century. The X-33 program was cancelled in 2001.
NASA Astrophysics Data System (ADS)
Ghenai, C.; Bettayeb, M.
2017-11-01
Modelling, simulation, optimization and control strategies are used in this study to design a stand-alone solar PV/Fuel Cell/Battery/Generator hybrid power system to serve the electrical load of a commercial building. The main objective is to design an off grid energy system to meet the desired electric load of the commercial building with high renewable fraction, low emissions and low cost of energy. The goal is to manage the energy consumption of the building, reduce the associate cost and to switch from grid-tied fossil fuel power system to an off grid renewable and cleaner power system. Energy audit was performed in this study to determine the energy consumption of the building. Hourly simulations, modelling and optimization were performed to determine the performance and cost of the hybrid power configurations using different control strategies. The results show that the hybrid off grid solar PV/Fuel Cell/Generator/Battery/Inverter power system offers the best performance for the tested system architectures. From the total energy generated from the off grid hybrid power system, 73% is produced from the solar PV, 24% from the fuel cell and 3% from the backup Diesel generator. The produced power is used to meet all the AC load of the building without power shortage (<0.1%). The hybrid power system produces 18.2% excess power that can be used to serve the thermal load of the building. The proposed hybrid power system is sustainable, economically viable and environmentally friendly: High renewable fraction (66.1%), low levelized cost of energy (92 /MWh), and low carbon dioxide emissions (24 kg CO2/MWh) are achieved.
Design, Build, and Test a Hand-held GPS Interference Detector
2008-09-01
TA 25* TA 26 Palisades Rappel Site TA 27 MOUT Site South Land Nav Course TTB Ward TA 28 TA 29 88M Test...72 73 74 THIS PAGE INTENTIONALLY LEFT BLANK 75 LIST OF REFERENCES Agilent Technolgies. " Cell Power, AWGN (Additive White Gaussian Noise
10. Credit USAF, 7 September 1945. Original housed in the ...
10. Credit USAF, 7 September 1945. Original housed in the Muroc Flight Test Base, Unit History, 1 September 1942 - 30 June 1945. Alfred F. Simpson Historical Research Agency. United States Air Force. Maxwell AFB, Alabama. View looks northwest into jet engine test cell located on aircraft apron southeast of Building 4305. In background of photo can be seen doors of Unicon Portable Hangar on left, and southeast end of Building T-l Bachelor Officers' Quarters ("Desert Rat Hotel"). This view emphasizes the hangar's role as a test facility for developing and testing aircraft and aircraft systems, not simply as a "garage" for aircraft. - Edwards Air Force Base, North Base, Unicon Portable Hangar, First & C Streets, Boron, Kern County, CA
Component qualification and initial build of the AGT 100 advanced automotive gas turbine
NASA Technical Reports Server (NTRS)
Johnson, R. A.
1983-01-01
In advance of initial dynamometer testing of the AGT 100 engine, all prime components and subsystems were bench/rig tested. Included were compressor, combustor, turbines, regenerator, ceramic components, and electronic control system. Results are briefly reviewed. Initial engine buildup was completed and rolled-out for test cell installation in July 1982. Shakedown testing included motoring and sequential firing of the combustor's three fuel nozzles.
Altitude Test Cell in the Four Burner Area
1947-10-21
One of the two altitude simulating-test chambers in Engine Research Building at the National Advisory Committee for Aeronautics (NACA) Lewis Flight Propulsion Laboratory. The two chambers were collectively referred to as the Four Burner Area. NACA Lewis’ Altitude Wind Tunnel was the nation’s first major facility used for testing full-scale engines in conditions that realistically simulated actual flight. The wind tunnel was such a success in the mid-1940s that there was a backlog of engines waiting to be tested. The Four Burner chambers were quickly built in 1946 and 1947 to ease the Altitude Wind Tunnel’s congested schedule. The Four Burner Area was located in the southwest wing of the massive Engine Research Building, across the road from the Altitude Wind Tunnel. The two chambers were 10 feet in diameter and 60 feet long. The refrigeration equipment produced the temperatures and the exhauster equipment created the low pressures present at altitudes up to 60,000 feet. In 1947 the Rolls Royce Nene was the first engine tested in the new facility. The mechanic in this photograph is installing a General Electric J-35 engine. Over the next ten years, a variety of studies were conducted using the General Electric J-47 and Wright Aeronautical J-65 turbojets. The two test cells were occasionally used for rocket engines between 1957 and 1959, but other facilities were better suited to the rocket engine testing. The Four Burner Area was shutdown in 1959. After years of inactivity, the facility was removed from the Engine Research Building in late 1973 in order to create the High Temperature and Pressure Combustor Test Facility.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Harvey, Scott D.; He, Lijian; Wahl, Jon H.
2012-08-30
This study provides a preliminary evaluation of the Field and Laboratory Emission Cell (FLEC) for its suitability for sampling building materials for toxic compounds and their associated impurities and residues that might remain after a terrorist chemical attack. Chemical warfare (CW) agents and toxic industrial chemicals were represented by a range of test probes that included CW surrogates. The test probes encompassed the acid-base properties, volatilities, and polarities of the expected chemical agents and residual compounds. Results indicated that dissipation of the test probes depended heavily on the underlying material. Near complete dissipation of almost all test probes occurred frommore » galvanized stainless steel within 3.0 hrs, whereas far stronger retention with concomitant slower release was observed for vinyl composition floor tiles. The test probes displayed immediated permanence on Teflon. FLEC sampling was further evaluated by profiling residues remaining after the evaporation of 2-chloroethyl ethyl sulfide, a sulfur mustard simulant. This study lays the groundwork for the eventual goal of applying this sampling approach for collection of forensic attribution signatures that remain after a terrorist chemical attack.« less
Development and Testing of Shingle-type Solar Cell Modules
NASA Technical Reports Server (NTRS)
Shepard, N. F., Jr.
1979-01-01
The design, development, fabrication and testing of a shingle-type terrestrial solar cell module which produces 98 watts/sq m of exposed module area at 1 kW/sq m insolation and 61 C are reported. These modules make it possible to easily incorporate photovoltaic power generation into the sloping roofs of residential or commercial buildings by simply nailing the modules to the plywood roof sheathing. This design consists of nineteen series-connected 53 mm diameter solar cells arranged in a closely packed hexagon configuration. These cells are individually bonded to the embossed surface of a 3 mm thick thermally tempered hexagon-shaped piece of glass. Polyvinyl butyral is used as the laminating adhesive.
Solar breeder: Energy payback time for silicon photovoltaic systems
NASA Technical Reports Server (NTRS)
Lindmayer, J.
1977-01-01
The energy expenditures of the prevailing manufacturing technology of terrestrial photovoltaic cells and panels were evaluated, including silicon reduction, silicon refinement, crystal growth, cell processing and panel building. Energy expenditures include direct energy, indirect energy, and energy in the form of equipment and overhead expenses. Payback times were development using a conventional solar cell as a test vehicle which allows for the comparison of its energy generating capability with the energies expended during the production process. It was found that the energy payback time for a typical solar panel produced by the prevailing technology is 6.4 years. Furthermore, this value drops to 3.8 years under more favorable conditions. Moreover, since the major energy use reductions in terrestrial manufacturing have occurred in cell processing, this payback time directly illustrates the areas where major future energy reductions can be made -- silicon refinement, crystal growth, and panel building.
Facility Decontamination and Decommissioning Program Surveillance and Maintenance Plan, Revision 2
DOE Office of Scientific and Technical Information (OSTI.GOV)
Poderis, Reed J.; King, Rebecca A.
This Surveillance and Maintenance (S&M) Plan describes the activities performed between deactivation and final decommissioning of the following facilities located on the Nevada National Security Site, as documented in the Federal Facility Agreement and Consent Order under the Industrial Sites program as decontamination and decommissioning sites: ? Engine Maintenance, Assembly, and Disassembly (EMAD) Facility: o EMAD Building (Building 25-3900) o Locomotive Storage Shed (Building 25-3901) ? Test Cell C (TCC) Facility: o Equipment Building (Building 25-3220) o Motor Drive Building (Building 25-3230) o Pump Shop (Building 25-3231) o Cryogenic Lab (Building 25-3232) o Ancillary Structures (e.g., dewars, water tower, piping,more » tanks) These facilities have been declared excess and are in various stages of deactivation (low-risk, long-term stewardship disposition state). This S&M Plan establishes and implements a solid, cost-effective, and balanced S&M program consistent with federal, state, and regulatory requirements. A graded approach is used to plan and conduct S&M activities. The goal is to maintain the facilities in a safe condition in a cost-effective manner until their final end state is achieved. This plan accomplishes the following: ? Establishes S&M objectives and framework ? Identifies programmatic guidance for S&M activities to be conducted by National Security Technologies, LLC, for the U.S. Department of Energy, National Nuclear Security Administration Nevada Field Office (NNSA/NFO) ? Provides present facility condition information and identifies hazards ? Identifies facility-specific S&M activities to be performed and their frequency ? Identifies regulatory drivers, NNSA/NFO policies and procedures, and best management practices that necessitate implementation of S&M activities ? Provides criteria and frequencies for revisions and updates ? Establishes the process for identifying and dispositioning a condition that has not been previously identified or documented ? Provides instructions for implementing annual S&M inspections and activities The following facilities that were included in Revision 1 of this plan have reached final disposition and are no longer in the S&M program: ? Reactor Maintenance, Assembly, and Disassembly Facility, Building 25-3110 ? Test Cell A Facility, Building 25-3113 ? TCC Facility, Building 25-3210 ? Pluto Disassembly Facility, Building 26-2201 ? Super Kukla Facility, Building 27-5400« less
Interior building details of Building A, dungeon cell adjacent to ...
Interior building details of Building A, dungeon cell adjacent to northwest cell: granite and brick threshold, poured concrete floors, plastered finished walls, vaulted veiling; northwesterly view - San Quentin State Prison, Building 22, Point San Quentin, San Quentin, Marin County, CA
Shielding analyses for repetitive high energy pulsed power accelerators
NASA Astrophysics Data System (ADS)
Jow, H. N.; Rao, D. V.
Sandia National Laboratories (SNL) designs, tests and operates a variety of accelerators that generate large amounts of high energy Bremsstrahlung radiation over an extended time. Typically, groups of similar accelerators are housed in a large building that is inaccessible to the general public. To facilitate independent operation of each accelerator, test cells are constructed around each accelerator to shield it from the radiation workers occupying surrounding test cells and work-areas. These test cells, about 9 ft. high, are constructed of high density concrete block walls that provide direct radiation shielding. Above the target areas (radiation sources), lead or steel plates are used to minimize skyshine radiation. Space, accessibility and cost considerations impose certain restrictions on the design of these test cells. SNL Health Physics division is tasked to evaluate the adequacy of each test cell design and compare resultant dose rates with the design criteria stated in DOE Order 5480.11. In response, SNL Health Physics has undertaken an intensive effort to assess existing radiation shielding codes and compare their predictions against measured dose rates. This paper provides a summary of the effort and its results.
A Constraint Based Approach for Building Operationally Responsive Satellites
2008-09-01
Analysis . . . . . . . . . . . . . . . . . . . 8 RST Responsive Space Testbed . . . . . . . . . . . . . . . . . . 9 HWIL Hardware - In - Loop . . . . . . . . . . . . . . . . . . . . . . 9...Responsive Space Satellite Cell in RST is configured to deliver the simulated environment to Hardware - In - Loop (HWIL) Device Under Test (DUT). As shown in
Preliminary Measurements of Thin Film Solar Cells
1967-06-21
George Mazaris, works with an assistant to obtain the preliminary measurements of cadmium sulfide thin-film solar cells being tested in the Space Environmental Chamber at the National Aeronautics and Space Administration (NASA) Lewis Research Center. Lewis’ Photovoltaic Fundamentals Section was investigating thin-film alternatives to the standard rigid and fragile solar cells. The cadmium sulfide semiconductors were placed in a light, metallized substrate that could be rolled or furled during launch. The main advantage of the thin-film solar cells was their reduced weight. Lewis researchers, however, were still working on improving the performance of the semiconductor. The new thin-film solar cells were tested in a space simulation chamber in the CW-6 test cell in the Engine Research Building. The chamber created a simulated altitude of 200 miles. Sunlight was simulated by a 5000-watt xenon light. Some two dozen cells were exposed to 15 minutes of light followed by 15 minutes of darkness to test their durability in the constantly changing illumination of Earth orbit. This photograph was taken for use in a NASA recruiting publication.
Churgin, Matthew A.; He, Liping; Murray, John I.; Fang-Yen, Christopher
2014-01-01
The spatial and temporal control of transgene expression is an important tool in C. elegans biology. We previously described a method for evoking gene expression in arbitrary cells by using a focused pulsed infrared laser to induce a heat shock response (Churgin et al 2013). Here we describe detailed methods for building and testing a system for performing single-cell heat shock. Steps include setting up the laser and associated components, coupling the laser beam to a microscope, and testing heat shock protocols. All steps can be carried out using readily available off-the-shelf components. PMID:24835576
Evaluation of sampling methods for toxicological testing of indoor air particulate matter.
Tirkkonen, Jenni; Täubel, Martin; Hirvonen, Maija-Riitta; Leppänen, Hanna; Lindsley, William G; Chen, Bean T; Hyvärinen, Anne; Huttunen, Kati
2016-09-01
There is a need for toxicity tests capable of recognizing indoor environments with compromised air quality, especially in the context of moisture damage. One of the key issues is sampling, which should both provide meaningful material for analyses and fulfill requirements imposed by practitioners using toxicity tests for health risk assessment. We aimed to evaluate different existing methods of sampling indoor particulate matter (PM) to develop a suitable sampling strategy for a toxicological assay. During three sampling campaigns in moisture-damaged and non-damaged school buildings, we evaluated one passive and three active sampling methods: the Settled Dust Box (SDB), the Button Aerosol Sampler, the Harvard Impactor and the National Institute for Occupational Safety and Health (NIOSH) Bioaerosol Cyclone Sampler. Mouse RAW264.7 macrophages were exposed to particle suspensions and cell metabolic activity (CMA), production of nitric oxide (NO) and tumor necrosis factor (TNFα) were determined after 24 h of exposure. The repeatability of the toxicological analyses was very good for all tested sampler types. Variability within the schools was found to be high especially between different classrooms in the moisture-damaged school. Passively collected settled dust and PM collected actively with the NIOSH Sampler (Stage 1) caused a clear response in exposed cells. The results suggested the higher relative immunotoxicological activity of dust from the moisture-damaged school. The NIOSH Sampler is a promising candidate for the collection of size-fractionated PM to be used in toxicity testing. The applicability of such sampling strategy in grading moisture damage severity in buildings needs to be developed further in a larger cohort of buildings.
SPERTI Instrument Cell Building (PER606) elevation; plan of Guard House ...
SPERT-I Instrument Cell Building (PER-606) elevation; plan of Guard House (PER-607); elevations for Pit Building (PER-605) southwest, southeast, and northeast sides. Earthen shield is mounded between back wall of Instrument Cell Building and the southwest elevation of Pit Building. Detail of filtered louver in door of Instrument Cell Building. Idaho Operations Office PER-605-IDO-3. INEEL index no. 761-0605-00-396-109183 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID
10. Interior view of control room in Components Test Laboratory ...
10. Interior view of control room in Components Test Laboratory (T-27), looking east. The control room is located in the center of the building and abuts the Test Cell 8, 9, and 10 and equipment room wings. Photograph shows upgraded instrumentation, piping, and technological modifications installed in 1997-99 to accommodate component testing requirements for the Atlas V missile. - Air Force Plant PJKS, Systems Integration Laboratory, Components Test Laboratory, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
Hubble Space Telescope Battery Capacity Update
NASA Technical Reports Server (NTRS)
Hollandsworth, Roger; Armantrout, Jon; Rao, Gopalakrishna M.
2007-01-01
Orbital battery performance for the Hubble Space Telescope is discussed and battery life is predicted which supports decision to replace orbital batteries by 2009-2010 timeframe. Ground characterization testing of cells from the replacement battery build is discussed, with comparison of data from battery capacity characterization with cell studies of Cycle Life and 60% Stress Test at the Naval Weapons Surface Center (NWSC)-Crane, and cell Cycle Life testing at the Marshal Space Flight Center (MSFC). The contents of this presentation includes an update to the performance of the on-orbit batteries, as well as a discussion of the HST Service Mission 4 (SM4) batteries manufactured in 1996 and activated in 2000, and a second set of SM4 backup replacement batteries which began manufacture Jan 11, 2007, with delivery scheduled for July 2008.
A 65 Ah rechargeable lithium molybdenum disulfide battery
NASA Technical Reports Server (NTRS)
Brandt, K.
1986-01-01
A rechargeable lithium molybdenum disulfide battery which has a number of superior performance characteristics which includes a high energy density, a high power density, and a long charge retention time was developed. The first cell sizes developed included a C size cell and an AA size cell. Over the last two years, a project to demonstrate the feasibility of the scale up to this technology to a BC size cell with 65 Ah capacity was undertaken. The objective was to develop, build, and test a .6 kWh storage battery consisting of 6 BC cells in series.
Rapid cell-free forward engineering of novel genetic ring oscillators
Niederholtmeyer, Henrike; Sun, Zachary Z; Hori, Yutaka; Yeung, Enoch; Verpoorte, Amanda; Murray, Richard M; Maerkl, Sebastian J
2015-01-01
While complex dynamic biological networks control gene expression in all living organisms, the forward engineering of comparable synthetic networks remains challenging. The current paradigm of characterizing synthetic networks in cells results in lengthy design-build-test cycles, minimal data collection, and poor quantitative characterization. Cell-free systems are appealing alternative environments, but it remains questionable whether biological networks behave similarly in cell-free systems and in cells. We characterized in a cell-free system the ‘repressilator’, a three-node synthetic oscillator. We then engineered novel three, four, and five-gene ring architectures, from characterization of circuit components to rapid analysis of complete networks. When implemented in cells, our novel 3-node networks produced population-wide oscillations and 95% of 5-node oscillator cells oscillated for up to 72 hr. Oscillation periods in cells matched the cell-free system results for all networks tested. An alternate forward engineering paradigm using cell-free systems can thus accurately capture cellular behavior. DOI: http://dx.doi.org/10.7554/eLife.09771.001 PMID:26430766
CONTEXTUAL AERIAL VIEW OF "COLD" NORTH HALF OF MTR COMPLEX. ...
CONTEXTUAL AERIAL VIEW OF "COLD" NORTH HALF OF MTR COMPLEX. CAMERA FACING EASTERLY. FOREGROUND CORNER CONTAINS OIL STORAGE TANKS. WATER TANKS AND WELL HOUSES ARE BEYOND THEM TO THE LEFT. LARGE LIGHT-COLORED BUILDING IN CENTER OF VIEW IS STEAM PLANT. DEMINERALIZER AND WATER STORAGE TANK ARE BEYOND. SIX-CELL COOLING TOWER AND ITS PUMP HOUSE ARE ABOVE IT IN VIEW. SERVICE BUILDINGS INCLUDING CANTEEN ARE ON NORTH SIDE OF ROAD. "EXCLUSION" AREA IS BEYOND ROAD. COMPARE LOCATION OF EXCLUSION-AREA GATE WITH PHOTO ID-33-G-202. INL NEGATIVE NO. 3608. Unknown Photographer, 10/30/1951 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
Lee, Jia-Ying Joey; Miller, James Alastair; Basu, Sreetama; Kee, Ting-Zhen Vanessa; Loo, Lit-Hsin
2018-06-01
Human lungs are susceptible to the toxicity induced by soluble xenobiotics. However, the direct cellular effects of many pulmonotoxic chemicals are not always clear, and thus, a general in vitro assay for testing pulmonotoxicity applicable to a wide variety of chemicals is not currently available. Here, we report a study that uses high-throughput imaging and artificial intelligence to build an in vitro pulmonotoxicity assay by automatically comparing and selecting human lung-cell lines and their associated quantitative phenotypic features most predictive of in vivo pulmonotoxicity. This approach is called "High-throughput In vitro Phenotypic Profiling for Toxicity Prediction" (HIPPTox). We found that the resulting assay based on two phenotypic features of a human bronchial epithelial cell line, BEAS-2B, can accurately classify 33 reference chemicals with human pulmonotoxicity information (88.8% balance accuracy, 84.6% sensitivity, and 93.0% specificity). In comparison, the predictivity of a standard cell-viability assay on the same set of chemicals is much lower (77.1% balanced accuracy, 84.6% sensitivity, and 69.5% specificity). We also used the assay to evaluate 17 additional test chemicals with unknown/unclear human pulmonotoxicity, and experimentally confirmed that many of the pulmonotoxic reference and predicted-positive test chemicals induce DNA strand breaks and/or activation of the DNA-damage response (DDR) pathway. Therefore, HIPPTox helps us to uncover these common modes-of-action of pulmonotoxic chemicals. HIPPTox may also be applied to other cell types or models, and accelerate the development of predictive in vitro assays for other cell-type- or organ-specific toxicities.
Draftsmen at Work during Construction of the Aircraft Engine Research Laboratory
1942-09-21
The National Advisory Committee for Aeronautics (NACA) Aircraft Engine Research Laboratory was designed by a group of engineers at the Langley Memorial Aeronautical Laboratory in late 1940 and 1941. Under the guidance of Ernest Whitney, the men worked on drawings and calculations in a room above Langley’s Structural Research Laboratory. The main Aircraft Engine Research Laboratory design group originally consisted of approximately 30 engineers and draftsmen, but there were smaller groups working separately on specific facilities. The new engine lab would have six principal buildings: the Engine Research Building, hangar, Fuels and Lubricants Building, Administration Building, Propeller Test Stand, and Altitude Wind Tunnel. In December 1941 most of those working on the project transferred to Cleveland from Langley. Harrison Underwood and Charles Egan led 18 architectural, 26 machine equipment, 3 structural and 10 mechanical draftsmen. Initially these staff members were housed in temporary offices in the hangar. As sections of the four-acre Engine Research Building were completed in the summer of 1942, the design team began relocating there. The Engine Research Building contained a variety of test cells and laboratories to address virtually every aspect of piston engine research. It also contained a two-story office wing, seen in this photograph that would later house many of the powerplant research engineers.
Implications of scaling on static RAM bit cell stability and reliability
NASA Astrophysics Data System (ADS)
Coones, Mary Ann; Herr, Norm; Bormann, Al; Erington, Kent; Soorholtz, Vince; Sweeney, John; Phillips, Michael
1993-01-01
In order to lower manufacturing costs and increase performance, static random access memory (SRAM) bit cells are scaled progressively toward submicron geometries. The reliability of an SRAM is highly dependent on the bit cell stability. Smaller memory cells with less capacitance and restoring current make the array more susceptible to failures from defectivity, alpha hits, and other instabilities and leakage mechanisms. Improving long term reliability while migrating to higher density devices makes the task of building in and improving reliability increasingly difficult. Reliability requirements for high density SRAMs are very demanding with failure rates of less than 100 failures per billion device hours (100 FITs) being a common criteria. Design techniques for increasing bit cell stability and manufacturability must be implemented in order to build in this level of reliability. Several types of analyses are performed to benchmark the performance of the SRAM device. Examples of these analysis techniques which are presented here include DC parametric measurements of test structures, functional bit mapping of the circuit used to characterize the entire distribution of bits, electrical microprobing of weak and/or failing bits, and system and accelerated soft error rate measurements. These tests allow process and design improvements to be evaluated prior to implementation on the final product. These results are used to provide comprehensive bit cell characterization which can then be compared to device models and adjusted accordingly to provide optimized cell stability versus cell size for a particular technology. The result is designed in reliability which can be accomplished during the early stages of product development.
Cell module and fuel conditioner development
NASA Astrophysics Data System (ADS)
Hoover, D. Q., Jr.
1980-01-01
Components for the first 5 cell stack (no cooling plates) of the MK-2 design were fabricated. Preliminary specfications and designs for the components of a 23 cell MK-1 stack with four DIGAS cooling plates were developed. The MK-2 was selected as a bench mark design and a preliminary design of the facilities required for high rate manufacture of fuel cell modules was developed. Two stands for testing 5 cell stacks were built and design work for modifying existing stands and building new stands for 23 and 80 cell stacks was initiated. Design and procurement of components and materials for the catalyst test stand were completed and construction initiated. Work on the specifications of pipeline gas, tap water and recovered water and definition of equipment required for treatment was initiated. An innovative geometry for the reformer was conceived and modifications of the computer program to be used in its design were stated.
NASA Astrophysics Data System (ADS)
Shepard, N. F.
1980-03-01
The Block 4 shingle type module makes it possible to apply a photovoltaic array to the sloping roof of a residential building by simply nailing the overlapping hexagon shaped shingles to the plywood roof sheathing. This third-generation shingle module design consists of nineteen series connected 100 mm diameter solar cells which are arranged in a closely packed hexagon configuration to provide in excess of 75 watts/sq m of exposed module area under standard operating conditions. The solar cells are individually bonded to the embossed underside of a 4.4 mm thick thermally tempered piece of glass. An experimental silicone pottant was used as the transparent bonding adhesive between the cells and glass. The semi-flexible portion of each shingle module is a composite laminate construction consisting of an outer layer of FLEXSEAL bonded to an inner core of closed cell polyethylene foam. Silaprene is used as the substrate laminating adhesive. The module design has satisfactorily survived qualification testing program which includes 50 thermal cycles between -40 and +90 C, a seven day temperature-humidity exposure test, and a wind resistance test.
NASA Technical Reports Server (NTRS)
Shepard, N. F.
1980-01-01
The Block 4 shingle type module makes it possible to apply a photovoltaic array to the sloping roof of a residential building by simply nailing the overlapping hexagon shaped shingles to the plywood roof sheathing. This third-generation shingle module design consists of nineteen series connected 100 mm diameter solar cells which are arranged in a closely packed hexagon configuration to provide in excess of 75 watts/sq m of exposed module area under standard operating conditions. The solar cells are individually bonded to the embossed underside of a 4.4 mm thick thermally tempered piece of glass. An experimental silicone pottant was used as the transparent bonding adhesive between the cells and glass. The semi-flexible portion of each shingle module is a composite laminate construction consisting of an outer layer of FLEXSEAL bonded to an inner core of closed cell polyethylene foam. Silaprene is used as the substrate laminating adhesive. The module design has satisfactorily survived qualification testing program which includes 50 thermal cycles between -40 and +90 C, a seven day temperature-humidity exposure test, and a wind resistance test.
The Cell Collective: Toward an open and collaborative approach to systems biology
2012-01-01
Background Despite decades of new discoveries in biomedical research, the overwhelming complexity of cells has been a significant barrier to a fundamental understanding of how cells work as a whole. As such, the holistic study of biochemical pathways requires computer modeling. Due to the complexity of cells, it is not feasible for one person or group to model the cell in its entirety. Results The Cell Collective is a platform that allows the world-wide scientific community to create these models collectively. Its interface enables users to build and use models without specifying any mathematical equations or computer code - addressing one of the major hurdles with computational research. In addition, this platform allows scientists to simulate and analyze the models in real-time on the web, including the ability to simulate loss/gain of function and test what-if scenarios in real time. Conclusions The Cell Collective is a web-based platform that enables laboratory scientists from across the globe to collaboratively build large-scale models of various biological processes, and simulate/analyze them in real time. In this manuscript, we show examples of its application to a large-scale model of signal transduction. PMID:22871178
31. SOUTH PLANT NORTHERN EDGE, SHOWING CELL BUILDING (BUILDING 242) ...
31. SOUTH PLANT NORTHERN EDGE, SHOWING CELL BUILDING (BUILDING 242) AT LEFT, LABORATORY (BUILDING 241) AT CENTER AND CAUSTIC FUSION PLANT (BUILDING 254) AT RIGHT. VIEW TO SOUTHWEST. - Rocky Mountain Arsenal, Bounded by Ninety-sixth Avenue & Fifty-sixth Avenue, Buckley Road, Quebec Street & Colorado Highway 2, Commerce City, Adams County, CO
IET. Aerial view of snaptran destructive experiment in 1964. Camera ...
IET. Aerial view of snaptran destructive experiment in 1964. Camera facing north. Test cell building (TAN-624) is positioned away from coupling station. Weather tower in right foreground. Divided duct just beyond coupling station. Air intake structure on south side of shielded control room. Experiment is on dolly at coupling station. Date: 1964. INEEL negative no. 64-1736 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID
NASA Technical Reports Server (NTRS)
1986-01-01
The main objective was to design and build a minimum of three photovoltaic test panels for plasma interaction experiments. These experiments are intended to provide data on the interactions between high-voltage solar arrays and the space plasma environment. Data gathered will significantly contribute to the development of design criteria for the space station solar arrays. Electrical isolation between the solar cell strings and the module mounting plate is required for high-voltage bias.
NASA Technical Reports Server (NTRS)
Pitts, E. R.
1981-01-01
Program converts cell-net data into logic-gate models for use in test and simulation programs. Input consists of either Place, Route, and Fold (PRF) or Place-and-Route-in-Two-Dimensions (PR2D) layout data deck. Output consists of either Test Pattern Generator (TPG) or Logic-Simulation (LOGSIM) logic circuitry data deck. Designer needs to build only logic-gate-model circuit description since program acts as translator. Language is FORTRAN IV.
NASA Astrophysics Data System (ADS)
Choi, Young Kwan; Lee, Jae Hyeong
2015-09-01
In this research, a facility was constructed and its performance was analyzed to improve the energy efficiency of a vertical-type water treatment building. After the design and construction of a fixed tilt Photovoltaic in Building (PVIB) on the rooftop using a crystalline silicon solar cell module and photovoltaic generator integrated with the building by using a Building Integrated Photovoltaic System (BIPV), a thin-film module on the rooftop and outer wall of water treatment building, and the generation efficiency was analyzed. Also, a DC distribution was established for use of a brushless DC (BLDC) pump motor, and the existing lighting-facility-based manual on-off method was turned into a system for energy conservation by controlling light emitting diode (LED) through a wireless motion sensor and dimming control. In addition, a Building Energy Management System (BEMS) for a real-time analysis of the energy efficiency for a vertical0type water treatment building was prepared and tested. The vertical-type water treatment building developed in this study is currently operating the BEMS. The vertical-type water treatment building reported in this paper is expected to reduce energy consumption by about 30% compared to existing water treatment systems.
4. VIEW TO THE NORTHWEST OF THE COLD BAY ON ...
4. VIEW TO THE NORTHWEST OF THE COLD BAY ON THE NORTH (RIGHT) AND THE POST-MORTEM CELLS ON THE SOUTH (LEFT). ALSO ILLUSTRATED ARE THE DIFFERENT ROOF HEIGHTS OF THE BUILDING. - Nevada Test Site, Engine Maintenance Assembly & Disassembly Facility, Area 25, Jackass Flats, Mercury, Nye County, NV
Application of self-balanced loading test to socketed pile in weak rock
NASA Astrophysics Data System (ADS)
Cheng, Ye; Gong, Weiming; Dai, Guoliang; Wu, JingKun
2008-11-01
Method of self-balanced loading test differs from the traditional methods of pile test. The key equipment of the test is a cell. The cell specially designed is used to exert load which is placed in pile body. During the test, displacement values of the top plate and the bottom plate of the cell are recorded according to every level of load. So Q-S curves can be obtained. In terms of test results, the bearing capacity of pile can be judged. Equipments of the test are simply and cost of it is low. Under some special conditions, the method will take a great advantage. In Guangxi Province, tertiary mudstone distributes widely which is typical weak rock. It is usually chosen as the bearing stratum of pile foundation. In order to make full use of its high bearing capacity, pile is generally designed as belled pile. Foundations of two high-rise buildings which are close to each other are made up of belled socketed piles in weak rock. To obtain the bearing capacity of the belled socketed pile in weak rock, loading test in situ should be taken since it is not reasonable that experimental compression strength of the mudstone is used for design. The self-balanced loading test was applied to eight piles of two buildings. To get the best test effect, the assembly of cell should be taken different modes in terms of the depth that pile socketed in rock and the dimension of the enlarged toe. The assembly of cells had been taken three modes, and tests were carried on successfully. By the self-balanced loading test, the large bearing capacities of belled socketed piles were obtained. Several key parameters required in design were achieved from the tests. For the data of tests had been analyzed, the bearing performance of pile tip, pile side and whole pile was revealed. It is further realized that the bearing capacity of belled socketed pile in the mudstone will decrease after the mudstone it socketed in has been immerged. Among kinds of mineral ingredient in the mudstone, montmorillonite is much. And in the size composition, content of cosmid is high. For specific surface area of cosmid is large and water intake capacity of it is strong, water content has great effect on strength of the mudstone. Along with water content increasing, strength of the mudstone declines nonlinear apparently. Since effective measures had been taken, the mudstone was prohibited from being immerged during construction. And valuable experience has been accumulated for similar projects construction henceforth.
Weißmann, Volker; Drescher, Philipp; Seitz, Hermann; Hansmann, Harald; Bader, Rainer; Seyfarth, Anika; Klinder, Annett; Jonitz-Heincke, Anika
2018-05-29
Additive manufacturing of lightweight or functional structures by selective laser beam (SLM) or electron beam melting (EBM) is widespread, especially in the field of medical applications. SLM and EBM processes were applied to prepare Ti6Al4V test specimens with different surface orientations (0°, 45° and 90°). Roughness measurements of the surfaces were conducted and cell behavior on these surfaces was analyzed. Hence, human osteoblasts were seeded on test specimens to determine cell viability (metabolic activity, live-dead staining) and gene expression of collagen type 1 (Col1A1), matrix metalloprotease (MMP) 1 and its natural inhibitor, TIMP1, after 3 and 7 days. The surface orientation of specimens during the manufacturing process significantly influenced the roughness. Surface roughness showed significant impact on cellular viability, whereas differences between the time points day 3 and 7 were not found. Collagen type 1 mRNA synthesis rates in human osteoblasts were enhanced with increasing roughness. Both manufacturing techniques further influenced the induction of bone formation process in the cell culture. Moreover, the relationship between osteoblastic collagen type 1 mRNA synthesis rates and specimen orientation during the building process could be characterized by functional formulas. These findings are useful in the designing of biomedical applications and medical devices.
Salin, J T; Salkinoja-Salonen, M; Salin, P J; Nelo, K; Holma, T; Ohtonen, P; Syrjälä, H
2017-04-01
Indoor microbial toxicity is suspected to cause some building-related symptoms, but supporting epidemiological data are lacking. We examined whether the in vitro toxicity of indoor samples from school buildings was associated with work-related health symptoms (building-related symptoms, BRS). Administrators of the Helsinki City Real Estate Department selected 15 schools for the study, and a questionnaire on symptoms connected to work was sent to the teachers in the selected schools for voluntary completion. The cellular toxicity of classroom samples was determined by testing substances extracted from wiped indoor dust and by testing microbial biomass that was cultured on fallout plates. Boar sperm cells were used as indicator cells, and motility loss was the indicator for toxic effects. The effects were expressed as the half maximal effective concentration (EC 50 ) at which >50% of the exposed boar sperm cells were immobile compared to vehicle control. Completed symptom questionnaires were received from 232 teachers [median age, 43 years; 190 (82.3%) women] with a median time of 6 years working at their school. Samples from their classrooms were available and were assessed for cellular toxicity. The Poisson regression model showed that the impact of extracts of surface-wiped school classroom dust on teacher work-related BRS was 2.8-fold (95% CI: 1.6-4.9) higher in classrooms with a toxic threshold EC 50 of 6µgml -1 versus classrooms with insignificant EC 50 values (EC 50 >50µgml -1 ); P<0.001. The number of symptoms that were alleviated during vacation was higher in school classrooms with high sperm toxicity compared to less toxic sites; the RR was 1.9 (95% CI: 1.1-3.3, P=0.03) for wiped dust extracts. Teachers working in classrooms where the samples showed high sperm toxicity had more BRS. The boar sperm cell motility inhibition assay appears promising as a tool for demonstrating the presence of indoor substances associated with BRS. Copyright © 2017 Elsevier Inc. All rights reserved.
Data quality objectives for the initial fuel conditioning examinations
DOE Office of Scientific and Technical Information (OSTI.GOV)
Lawrence, L.A.
The Data Quality Objectives (DQOs) were established for the response of the first group of fuel samples shipped from the K West Basin to the Hanford 327 Building hot cells for examinations to the proposed Path Forward conditioning process. Controlled temperature and atmosphere furnace testing testing will establish performance parameters using the conditioning process (drying, sludge drying, hydride decomposition passivation) proposed by the Independent Technical Assessment (ITA) Team as the baseline.
Develop and test fuel cell powered on-site integrated total energy system
NASA Technical Reports Server (NTRS)
1983-01-01
Test results are given for a 5 kW stack and initial results for an integrated, grid connected system operating from methanol fuel. Site selection criteria are presented for future demonstration of a 50 or 100 kW OS/IES. Preliminary results are also given with approximate internal rates of return to the building owner. Progress in development and construction of a 50 kW modular methanol/steam reformer is reported.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Robertson, D.K.; Christian, J.E.
1985-01-01
Eight one-room test buildings, 20 ft (6.1 m) square and 7.5 ft (2.3 m) high, were constructed on a high desert site near Tesuque Pueblo, New Mexico, to study the influence of wall dynamic heat transfer characteristics on building heating requirements (the ''thermal mass effect''). The buildings are nominally identical except for the walls (adobe, concrete and masonry unit, wood-frame, and log) and are constructed so as to isolate the effects of the walls. The amount of mass in the walls varies from 240 lb/ft/sup 2/ (1171 kg/m/sup 2/) for the 2 ft (.61 m) thick adobe wall to 4.3more » lb/ft/sup 2/ (21 kg/m/sup 2/) for the insulated wood-frame wall. The roof, floor, and stem walls are all well insulated and the buildings were constructed with infiltration rates less than 0.4 air change per hour. The site is instrumented to record building component temperatures and heat fluxes, outside weather conditions, and heating energy use. Data were collected for two heating seasons from midwinter to late spring with the buildings in two configurations, with and without windows. Four computer codes were used to simulate the performance of the test buildings without windows, using site weather data. The codes used were DOE-2.1A, DOE-2.1C, BLAST, and DEROB. Each code was run by a different analyst. Simulations were done for midwinter, late winter, and spring. Two of the test cell comparisons are discussed; the insulated frame and an 11-in. (.28 m) adobe. This work presents a quantitative and qualitative critical comparison of the modeling and experimental results. Cumulative heating loads, wall heat fluxes, and air surface temperatures are compared, as well as input assumptions to the models. Explanations of differences and difficulties encountered are reported. The principal findings were that cumulative heating loads and the characteristic influences of wall thermal mass on hourly behavior were reproduced by the models.« less
Thermo-electrochemical evaluation of lithium-ion batteries for space applications
NASA Astrophysics Data System (ADS)
Walker, W.; Yayathi, S.; Shaw, J.; Ardebili, H.
2015-12-01
Advanced energy storage and power management systems designed through rigorous materials selection, testing and analysis processes are essential to ensuring mission longevity and success for space exploration applications. Comprehensive testing of Boston Power Swing 5300 lithium-ion (Li-ion) cells utilized by the National Aeronautics and Space Administration (NASA) to power humanoid robot Robonaut 2 (R2) is conducted to support the development of a test-correlated Thermal Desktop (TD) Systems Improved Numerical Differencing Analyzer (SINDA) (TD-S) model for evaluation of power system thermal performance. Temperature, current, working voltage and open circuit voltage measurements are taken during nominal charge-discharge operations to provide necessary characterization of the Swing 5300 cells for TD-S model correlation. Building from test data, embedded FORTRAN statements directly simulate Ohmic heat generation of the cells during charge-discharge as a function of surrounding temperature, local cell temperature and state of charge. The unique capability gained by using TD-S is demonstrated by simulating R2 battery thermal performance in example orbital environments for hypothetical extra-vehicular activities (EVA) exterior to a small satellite. Results provide necessary demonstration of this TD-S technique for thermo-electrochemical analysis of Li-ion cells operating in space environments.
Credit BG. View looking northeast at southwestern side of Test ...
Credit BG. View looking northeast at southwestern side of Test Stand "D" complex. Test Stand "D" workshop (Building 4222/E-23) is at left; shed to its immediate right is an entrance to underground tunnel system which interconnects all test stands. To the right of Test Stand "D" tower are four Clayton water-tube flash boilers once used in the Steam Generator Plant 4280/E-81 to power the vacuum ejector system at "D" and "C" stands. A corner of 4280/E-81 appears behind the boilers. Boilers were removed as part of stand dismantling program. The Dv (vertical vacuum) Test Cell is located in the Test Stand "D" tower, behind the sunscreen on the west side. The top of the tower contains a hoist for lifting or lowering rocket engines into the Dv Cell. Other equipment mounted in the tower is part of the steam-driven vacuum ejector system - Jet Propulsion Laboratory Edwards Facility, Test Stand D, Edwards Air Force Base, Boron, Kern County, CA
Economic competitiveness of fuel cell onsite integrated energy systems
NASA Technical Reports Server (NTRS)
Bollenbacher, G.
1983-01-01
The economic competitiveness of fuel cell onsite integrated energy systems (OS/IES) in residential and commercial buildings is examined. The analysis is carried out for three different buildings with each building assumed to be at three geographic locations spanning a range of climatic conditions. Numerous design options and operating strategies are evaluated and two economic criteria are used to measure economic performance. In general the results show that fuel cell OS/IES's are competitive in most regions of the country if the OS/IES is properly designed. The preferred design is grid connected, makes effective use of the fuel cell's thermal output, and has a fuel cell powerplant sized for the building's base electrical load.
A Barcoding Strategy Enabling Higher-Throughput Library Screening by Microscopy.
Chen, Robert; Rishi, Harneet S; Potapov, Vladimir; Yamada, Masaki R; Yeh, Vincent J; Chow, Thomas; Cheung, Celia L; Jones, Austin T; Johnson, Terry D; Keating, Amy E; DeLoache, William C; Dueber, John E
2015-11-20
Dramatic progress has been made in the design and build phases of the design-build-test cycle for engineering cells. However, the test phase usually limits throughput, as many outputs of interest are not amenable to rapid analytical measurements. For example, phenotypes such as motility, morphology, and subcellular localization can be readily measured by microscopy, but analysis of these phenotypes is notoriously slow. To increase throughput, we developed microscopy-readable barcodes (MiCodes) composed of fluorescent proteins targeted to discernible organelles. In this system, a unique barcode can be genetically linked to each library member, making possible the parallel analysis of phenotypes of interest via microscopy. As a first demonstration, we MiCoded a set of synthetic coiled-coil leucine zipper proteins to allow an 8 × 8 matrix to be tested for specific interactions in micrographs consisting of mixed populations of cells. A novel microscopy-readable two-hybrid fluorescence localization assay for probing candidate interactions in the cytosol was also developed using a bait protein targeted to the peroxisome and a prey protein tagged with a fluorescent protein. This work introduces a generalizable, scalable platform for making microscopy amenable to higher-throughput library screening experiments, thereby coupling the power of imaging with the utility of combinatorial search paradigms.
Mir Cooperative Solar Array Project Accelerated Life Thermal Cycling Test
NASA Technical Reports Server (NTRS)
Hoffman, David J.; Scheiman, David A.
1996-01-01
The Mir Cooperative Solar Array (MCSA) project was a joint U.S./Russian effort to build a photovoltaic (PV) solar array and deliver it to the Russian space station Mir. The MCSA will be used to increase the electrical power on Mir and provide PV array performance data in support of Phase 1 of the International Space Station. The MCSA was brought to Mir by space shuttle Atlantis in November 1995. This report describes an accelerated thermal life cycle test which was performed on two samples of the MCSA. In eight months time, two MCSA solar array 'mini' panel test articles were simultaneously put through 24,000 thermal cycles. There was no significant degradation in the structural integrity of the test articles and no electrical degradation, not including one cell damaged early and removed from consideration. The nature of the performance degradation caused by this one cell is briefly discussed. As a result of this test, changes were made to improve some aspects of the solar cell coupon-to-support frame interface on the flight unit. It was concluded from the results that the integration of the U.S. solar cell modules with the Russian support structure would be able to withstand at least 24,000 thermal cycles (4 years on-orbit). This was considered a successful development test.
5. Interior of Building 1042 (brig), cell, looking northeast ...
5. Interior of Building 1042 (brig), cell, looking northeast - Naval Air Station Chase Field, Building 1042, Ofstie Road, .6 mile South-Southeast of intersection of Texas State Highway 202 & Independence Street, Beeville, Bee County, TX
Detection of viruses in used ventilation filters from two large public buildings.
Goyal, Sagar M; Anantharaman, Senthilvelan; Ramakrishnan, M A; Sajja, Suchitra; Kim, Seung Won; Stanley, Nicholas J; Farnsworth, James E; Kuehn, Thomas H; Raynor, Peter C
2011-09-01
Viral and bacterial pathogens may be present in the air after being released from infected individuals and animals. Filters are installed in the heating, ventilation, and air-conditioning (HVAC) systems of buildings to protect ventilation equipment and maintain healthy indoor air quality. These filters process enormous volumes of air. This study was undertaken to determine the utility of sampling used ventilation filters to assess the types and concentrations of virus aerosols present in buildings. The HVAC filters from 2 large public buildings in Minneapolis and Seattle were sampled to determine the presence of human respiratory viruses and viruses with bioterrorism potential. Four air-handling units were selected from each building, and a total of 64 prefilters and final filters were tested for the presence of influenza A, influenza B, respiratory syncytial, corona, parainfluenza 1-3, adeno, orthopox, entero, Ebola, Marburg, Lassa fever, Machupo, eastern equine encephalitis, western equine encephalitis, and Venezuelan equine encephalitis viruses. Representative pieces of each filter were cut and eluted with a buffer solution. Attempts were made to detect viruses by inoculation of these eluates in cell cultures (Vero, MDCK, and RK-13) and specific pathogen-free embryonated chicken eggs. Two passages of eluates in cell cultures or these eggs did not reveal the presence of any live virus. The eluates were also examined by polymerase chain reaction or reverse-transcription polymerase chain reaction to detect the presence of viral DNA or RNA, respectively. Nine of the 64 filters tested were positive for influenza A virus, 2 filters were positive for influenza B virus, and 1 filter was positive for parainfluenza virus 1. These findings indicate that existing building HVAC filters may be used as a method of detection for airborne viruses. As integrated long-term bioaerosol sampling devices, they may yield valuable information on the epidemiology and aerobiology of viruses in air that can inform the development of methods to prevent airborne transmission of viruses and possible deterrents against the spread of bioterrorism agents. Copyright © 2011 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Mosby, Inc. All rights reserved.
Advanced Combustor in the Four Burner Area
1966-03-21
Engineer Frank Kutina and a National Aeronautics and Space Administration (NASA) mechanic examine the setup of an advanced combustor rig inside one of the test cells at the Lewis Research Center’s Four Burner Area in the Engine Research Building. Kutina, of the Research Operations Branch, served as go-between for the researchers and the mechanics. He helped develop the test configurations and get the hardware installed. At the time of this photograph, Lewis Center Director Abe Silverstein had just established the Airbreathing Engine Division to address the new propulsion of the 1960s. After nearly a decade of focusing almost exclusively on space, NASA Lewis began tackling issues relating to the new turbofan engine, noise reduction, energy efficiency, supersonic transport, and the never-ending quest for higher performance levels with smaller and more lightweight engines. The Airbreathing Engine Division’s Combustion Branch was dedicated to the study and mitigation of the high temperatures and pressures found in advanced combustor designs. These high temperatures and pressures could destroy engine components. The Lewis investigation included film cooling, diffuser flow, and jet mixing. Components were tested in smaller test cells, but a full-scale augmenting burner rig, seen here, was tested extensively in the Four Burner Area test cell.
Nuclear lamina builds tissues from the stem cell niche.
Chen, Haiyang; Zheng, Yixian
2014-01-01
Recent studies show that nuclear lamins, the type V intermediate filament proteins, are required for proper building of at least some organs. As the major structural components of the nuclear lamina found underneath the inner nuclear membranes, lamins are ubiquitously expressed in all animal cells. How the broadly expressed lamins support the building of specific tissues is not understood. By studying Drosophila testis, we have uncovered a mechanism by which lamin-B functions in the cyst stem cell (CySC) and its differentiated cyst cell, the cell types known to form the niche/microenvironment for the germline stem cells (GSC) and the developing germ line, to ensure testis organogenesis (1). In this extra view, we discuss some remaining questions and the implications of our findings in the understanding of how the ubiquitous nuclear lamina regulates tissue building in a context-dependent manner.
WASTE PACKAGE REMEDIATION SYSTEM DESCRIPTION DOCUMENT
DOE Office of Scientific and Technical Information (OSTI.GOV)
N.D. Sudan
2000-06-22
The Waste Package Remediation System remediates waste packages (WPs) and disposal containers (DCs) in one of two ways: preparation of rejected DC closure welds for repair or opening of the DC/WP. DCs are brought to the Waste Package Remediation System for preparation of rejected closure welds if testing of the closure weld by the Disposal Container Handling System indicates an unacceptable, but repairable, welding flaw. DC preparation of rejected closure welds will require removal of the weld in such a way that the Disposal Container Handling System may resume and complete the closure welding process. DCs/WPs are brought to themore » Waste Package Remediation System for opening if the Disposal Container Handling System testing of the DC closure weld indicates an unrepairable welding flaw, or if a WP is recovered from the subsurface repository because suspected damage to the WP or failure of the WP has occurred. DC/WP opening will require cutting of the DC/WP such that a temporary seal may be installed and the waste inside the DC/WP removed by another system. The system operates in a Waste Package Remediation System hot cell located in the Waste Handling Building that has direct access to the Disposal Container Handling System. One DC/WP at a time can be handled in the hot cell. The DC/WP arrives on a transfer cart, is positioned within the cell for system operations, and exits the cell without being removed from the cart. The system includes a wide variety of remotely operated components including a manipulator with hoist and/or jib crane, viewing systems, machine tools for opening WPs, and equipment used to perform pressure and gas composition sampling. Remotely operated equipment is designed to facilitate DC/WP decontamination and hot cell equipment maintenance, and interchangeable components are provided where appropriate. The Waste Package Remediation System interfaces with the Disposal Container Handling System for the receipt and transport of WPs and DCs. The Waste Handling Building System houses the system, and provides the facility, safety, and auxiliary systems required to support operations. The system receives power from the Waste Handling Building Electrical System. The system also interfaces with the various DC systems.« less
Safety modelling and testing of lithium-ion batteries in electrified vehicles
NASA Astrophysics Data System (ADS)
Deng, Jie; Bae, Chulheung; Marcicki, James; Masias, Alvaro; Miller, Theodore
2018-04-01
To optimize the safety of batteries, it is important to understand their behaviours when subjected to abuse conditions. Most early efforts in battery safety modelling focused on either one battery cell or a single field of interest such as mechanical or thermal failure. These efforts may not completely reflect the failure of batteries in automotive applications, where various physical processes can take place in a large number of cells simultaneously. In this Perspective, we review modelling and testing approaches for battery safety under abuse conditions. We then propose a general framework for large-scale multi-physics modelling and experimental work to address safety issues of automotive batteries in real-world applications. In particular, we consider modelling coupled mechanical, electrical, electrochemical and thermal behaviours of batteries, and explore strategies to extend simulations to the battery module and pack level. Moreover, we evaluate safety test approaches for an entire range of automotive hardware sets from cell to pack. We also discuss challenges in building this framework and directions for its future development.
A fuel cell energy storage system concept for the Space Station Freedom Extravehicular Mobility Unit
NASA Technical Reports Server (NTRS)
Adlhart, Otto J.; Rosso, Matthew J., Jr.; Marmolejo, Jose
1989-01-01
An update is given on work to design and build a Fuel Cell Energy Storage System (FCESS) bench-tested unit for the Space Station Freedom Extravehicular Mobility Unit (EMU). Fueled by oxygen and hydride-stored hydrogen, the FCESS is being considered as an alternative to the EMU zinc-silver oxide battery. Superior cycle life and quick recharge are the main attributes of FCESS. The design and performance of a nonventing, 28 V, 34 Ahr system with 7 amp rating are discussed.
A fuel cell energy storage system concept for the Space Station Freedom Extravehicular Mobility Unit
NASA Astrophysics Data System (ADS)
Adlhart, Otto J.; Rosso, Matthew J., Jr.; Marmolejo, Jose
1989-03-01
An update is given on work to design and build a Fuel Cell Energy Storage System (FCESS) bench-tested unit for the Space Station Freedom Extravehicular Mobility Unit (EMU). Fueled by oxygen and hydride-stored hydrogen, the FCESS is being considered as an alternative to the EMU zinc-silver oxide battery. Superior cycle life and quick recharge are the main attributes of FCESS. The design and performance of a nonventing, 28 V, 34 Ahr system with 7 amp rating are discussed.
Cell-free metabolic engineering: Biomanufacturing beyond the cell
DOE Office of Scientific and Technical Information (OSTI.GOV)
Dudley, QM; Karim, AS; Jewett, MC
2014-10-15
Industrial biotechnology and microbial metabolic engineering are poised to help meet the growing demand for sustainable, low-cost commodity chemicals and natural products, yet the fraction of biochemicals amenable to commercial production remains limited. Common problems afflicting the current state-of-the-art include low volumetric productivities, build-up of toxic intermediates or products, and byproduct losses via competing pathways. To overcome these limitations, cell-free metabolic engineering (CFME) is expanding the scope of the traditional bioengineering model by using in vitro ensembles of catalytic proteins prepared from purified enzymes or crude lysates of cells for the production of target products. In recent years, the unprecedentedmore » level of control and freedom of design, relative to in vivo systems, has inspired the development of engineering foundations for cell-free systems. These efforts have led to activation of long enzymatic pathways (>8 enzymes), near theoretical conversion yields, productivities greater than 100 mg L-1 h(-1), reaction scales of >100 L, and new directions in protein purification, spatial organization, and enzyme stability. In the coming years, CFME will offer exciting opportunities to: (i) debug and optimize biosynthetic pathways; (ii) carry out design-build-test iterations without re-engineering organisms; and (iii) perform molecular transformations when bioconversion yields, productivities, or cellular toxicity limit commercial feasibility.« less
Cell-Free Metabolic Engineering: Biomanufacturing beyond the cell
Dudley, Quentin M.; Karim, Ashty S.; Jewett, Michael C.
2014-01-01
Industrial biotechnology and microbial metabolic engineering are poised to help meet the growing demand for sustainable, low-cost commodity chemicals and natural products, yet the fraction of biochemicals amenable to commercial production remains limited. Common problems afflicting the current state-of-the-art include low volumetric productivities, build-up of toxic intermediates or products, and byproduct losses via competing pathways. To overcome these limitations, cell-free metabolic engineering (CFME) is expanding the scope of the traditional bioengineering model by using in vitro ensembles of catalytic proteins prepared from purified enzymes or crude lysates of cells for the production of target products. In recent years, the unprecedented level of control and freedom of design, relative to in vivo systems, has inspired the development of engineering foundations for cell-free systems. These efforts have led to activation of long enzymatic pathways (>8 enzymes), near theoretical conversion yields, productivities greater than 100 mg L−1 hr−1, reaction scales of >100L, and new directions in protein purification, spatial organization and enzyme stability. In the coming years, CFME will offer exciting opportunities to (i) debug and optimize biosynthetic pathways, (ii) carry out design-build-test iterations without re-engineering organisms, and (iii) perform molecular transformations when bioconversion yields, productivities, or cellular toxicity limit commercial feasibility. PMID:25319678
Radon Sampling, Building 54, Nellis AFB, NV
2012-07-13
BEF) performed radon testing in response to a concern of elevated radon levels in Building 54. The building was previously remediated to reduce the... TESTING METHODOLOGY: a. Test Scenario: Building 54 was chosen to test for radon gas levels. Radon detectors were placed in the test ...Consultative Letter 3. DATES COVERED (From – To) 22-24 March 2012 4. TITLE AND SUBTITLE Radon Sampling, Building 54, Nellis AFB, NV 5a. CONTRACT
Neuronize: a tool for building realistic neuronal cell morphologies
Brito, Juan P.; Mata, Susana; Bayona, Sofia; Pastor, Luis; DeFelipe, Javier; Benavides-Piccione, Ruth
2013-01-01
This study presents a tool, Neuronize, for building realistic three-dimensional models of neuronal cells from the morphological information extracted through computer-aided tracing applications. Neuronize consists of a set of methods designed to build 3D neural meshes that approximate the cell membrane at different resolution levels, allowing a balance to be reached between the complexity and the quality of the final model. The main contribution of the present study is the proposal of a novel approach to build a realistic and accurate 3D shape of the soma from the incomplete information stored in the digitally traced neuron, which usually consists of a 2D cell body contour. This technique is based on the deformation of an initial shape driven by the position and thickness of the first order dendrites. The addition of a set of spines along the dendrites completes the model, building a final 3D neuronal cell suitable for its visualization in a wide range of 3D environments. PMID:23761740
Neuronize: a tool for building realistic neuronal cell morphologies.
Brito, Juan P; Mata, Susana; Bayona, Sofia; Pastor, Luis; Defelipe, Javier; Benavides-Piccione, Ruth
2013-01-01
This study presents a tool, Neuronize, for building realistic three-dimensional models of neuronal cells from the morphological information extracted through computer-aided tracing applications. Neuronize consists of a set of methods designed to build 3D neural meshes that approximate the cell membrane at different resolution levels, allowing a balance to be reached between the complexity and the quality of the final model. The main contribution of the present study is the proposal of a novel approach to build a realistic and accurate 3D shape of the soma from the incomplete information stored in the digitally traced neuron, which usually consists of a 2D cell body contour. This technique is based on the deformation of an initial shape driven by the position and thickness of the first order dendrites. The addition of a set of spines along the dendrites completes the model, building a final 3D neuronal cell suitable for its visualization in a wide range of 3D environments.
2010-01-06
CAPE CANAVERAL, Fla. – In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, the external fuel tank for space shuttle Discovery's STS-131 mission, ET-135, is lowered into a test cell. The tank was delivered to Kennedy aboard the Pegasus barge from NASA's Michoud Assembly Facility on Dec. 26. The tank will remain in the test cell until it is transferred into a high bay for mating with the twin solid rocket boosters that will be used on the mission. Launch of the STS-131 mission to the International Space Station is targeted for March 18. For information on the STS-131 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts131/index.html. Photo credit: NASA/Glenn Benson
2010-01-06
CAPE CANAVERAL, Fla. – In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, a crane is enlisted to lift the external fuel tank for space shuttle Discovery's STS-131 mission, ET-135, into a test cell. The tank was delivered to Kennedy aboard the Pegasus barge from NASA's Michoud Assembly Facility on Dec. 26. The tank will remain in the test cell until it is transferred into a high bay for mating with the twin solid rocket boosters that will be used on the mission. Launch of the STS-131 mission to the International Space Station is targeted for March 18. For information on the STS-131 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts131/index.html. Photo credit: NASA/Glenn Benson
2010-01-06
CAPE CANAVERAL, Fla. – In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, the external fuel tank for space shuttle Discovery's STS-131 mission, ET-135, has arrived in its test cell. The tank was delivered to Kennedy aboard the Pegasus barge from NASA's Michoud Assembly Facility on Dec. 26. The tank will remain in the test cell until it is transferred into a high bay for mating with the twin solid rocket boosters that will be used on the mission. Launch of the STS-131 mission to the International Space Station is targeted for March 18. For information on the STS-131 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts131/index.html. Photo credit: NASA/Glenn Benson
Study of fuel cell on-site, integrated energy systems in residential/commercial applications
NASA Technical Reports Server (NTRS)
Wakefield, R. A.; Karamchetty, S.; Rand, R. H.; Ku, W. S.; Tekumalla, V.
1980-01-01
Three building applications were selected for a detailed study: a low rise apartment building; a retail store, and a hospital. Building design data were then specified for each application, based on the design and construction of typical, actual buildings. Finally, a computerized building loads analysis program was used to estimate hourly end use load profiles for each building. Conventional and fuel cell based energy systems were designed and simulated for each building in each location. Based on the results of a computer simulation of each energy system, levelized annual costs and annual energy consumptions were calculated for all systems.
Severe Accident Test Station Design Document
DOE Office of Scientific and Technical Information (OSTI.GOV)
Snead, Mary A.; Yan, Yong; Howell, Michael
The purpose of the ORNL severe accident test station (SATS) is to provide a platform for evaluation of advanced fuels under projected beyond design basis accident (BDBA) conditions. The SATS delivers the capability to map the behavior of advanced fuels concepts under accident scenarios across various temperature and pressure profiles, steam and steam-hydrogen gas mixtures, and thermal shock. The overall facility will include parallel capabilities for examination of fuels and irradiated materials (in-cell) and non-irradiated materials (out-of-cell) at BDBA conditions as well as design basis accident (DBA) or loss of coolant accident (LOCA) conditions. Also, a supporting analytical infrastructure tomore » provide the data-needs for the fuel-modeling components of the Fuel Cycle Research and Development (FCRD) program will be put in place in a parallel manner. This design report contains the information for the first, second and third phases of design and construction of the SATS. The first phase consisted of the design and construction of an out-of-cell BDBA module intended for examination of non-irradiated materials. The second phase of this work was to construct the BDBA in-cell module to test irradiated fuels and materials as well as the module for DBA (i.e. LOCA) testing out-of-cell, The third phase was to build the in-cell DBA module. The details of the design constraints and requirements for the in-cell facility have been closely captured during the deployment of the out-of-cell SATS modules to ensure effective future implementation of the in-cell modules.« less
A Space Testbed for Photovoltaics
NASA Technical Reports Server (NTRS)
Landis, Geoffrey A.; Bailey, Sheila G.
1998-01-01
The Ohio Aerospace Institute and the NASA Lewis Research Center are designing and building a solar-cell calibration facility, the Photovoltaic Engineering Testbed (PET) to fly on the International Space Station to test advanced solar cell types in the space environment. A wide variety of advanced solar cell types have become available in the last decade. Some of these solar cells offer more than twice the power per unit area of the silicon cells used for the space station power system. They also offer the possibilities of lower cost, lighter weight, and longer lifetime. The purpose of the PET facility is to reduce the cost of validating new technologies and bringing them to spaceflight readiness. The facility will be used for three primary functions: calibration, measurement, and qualification. It is scheduled to be launched in June of 2002.
Photovoltaic building sheathing element with anti-slide features
Keenihan, James R.; Langmaid, Joseph A.; Lopez, Leonardo C.
2015-09-08
The present invention is premised` upon an assembly that includes at least a photovoltaic building sheathing element capable of being affixed on a building structure, the photovoltaic building sheathing element. The element including a photovoltaic cell assembly, a body portion attached to one or more portions of the photovoltaic cell assembly; and at feast a first and a second connector assembly capable of directly or indirectly electrically connecting the photovoltaic cell assembly to one or more adjoining devices; wherein the body portion includes one or more geometric features adapted to engage a vertically adjoining device before installation.
Small molecule absorption by PDMS in the context of drug response bioassays.
van Meer, B J; de Vries, H; Firth, K S A; van Weerd, J; Tertoolen, L G J; Karperien, H B J; Jonkheijm, P; Denning, C; IJzerman, A P; Mummery, C L
2017-01-08
The polymer polydimethylsiloxane (PDMS) is widely used to build microfluidic devices compatible with cell culture. Whilst convenient in manufacture, PDMS has the disadvantage that it can absorb small molecules such as drugs. In microfluidic devices like "Organs-on-Chip", designed to examine cell behavior and test the effects of drugs, this might impact drug bioavailability. Here we developed an assay to compare the absorption of a test set of four cardiac drugs by PDMS based on measuring the residual non-absorbed compound by High Pressure Liquid Chromatography (HPLC). We showed that absorption was variable and time dependent and not determined exclusively by hydrophobicity as claimed previously. We demonstrated that two commercially available lipophilic coatings and the presence of cells affected absorption. The use of lipophilic coatings may be useful in preventing small molecule absorption by PDMS. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.
NASA Technical Reports Server (NTRS)
Berliner, Aaron J.
2013-01-01
Although methods in the design-build-test life cycle of the synthetic biology field have grown rapidly, the expansion has been non-uniform. The design and build stages in development have seen innovations in the form of biological CAD and more efficient means for building DNA, RNA, and other biological constructs. The testing phase of the cycle remains in need of innovation. Presented will be both a theoretical abstraction of biological measurement and a practical demonstration of a microfluidics-based platform for characterizing synthetic biological phenomena. Such a platform demonstrates a design of additive manufacturing (3D printing) for construction of a microbial fuel cell (MFC) to be used in experiments carried out in space. First, the biocompatibility of the polypropylene chassis will be demonstrated. The novel MFCs will be cheaper, and faster to make and iterate through designs. The novel design will contain a manifold switchingdistribution system and an integrated in-chip set of reagent reservoirs fabricated via 3D printing. The automated nature of the 3D printing yields itself to higher resolution switching valves and leads to smaller sized payloads, lower cost, reduced power and a standardized platform for synthetic biology unit tests on Earth and in space. It will be demonstrated that the application of unit testing in synthetic biology will lead to the automatic construction and validation of desired constructs. Unit testing methodologies offer benefits of preemptive problem identification, change of facility, simplicity of integration, ease of documentation, and separation of interface from implementation, and automated design.
Thermoelectric converter for SP-100 space reactor power system
NASA Technical Reports Server (NTRS)
Terrill, W. R.; Haley, V. F.
1986-01-01
Conductively coupling the thermoelectric converter to the heat source and the radiator maximizes the utilization of the reactor and radiator temperatures and thereby minimizes the power system weight. This paper presents the design for the converter and the individual thermoelectric cells that are the building block modules for the converter. It also summarizes progress on the fabrication of initial cells and the results obtained from the preparation of a manufacturing plan. The design developed for the SP-100 system utilizes thermally conductive compliant pads that can absorb the displacement and distortion caused by the combinations of temperatures and thermal expansion coefficients. The converter and cell designs provided a 100 kWe system which met the system requirements. Initial cells were fabricated and tested.
1. MISSILE TEST AND ASSEMBLY BUILDING, FRONT, LOOKING SOUTH. ...
1. MISSILE TEST AND ASSEMBLY BUILDING, FRONT, LOOKING SOUTH. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL
LPT. Shield test facility assembly and test building (TAN646), south ...
LPT. Shield test facility assembly and test building (TAN-646), south facade. Camera facing north. High-bay section is pool room. Single-story section at right is control building (TAN-645). Small metal building is post-1970 addition. INEEL negative no. HD-40-7-3 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID
NASA Astrophysics Data System (ADS)
DiMola, Ashley M.
Buildings account for over 18% of the world's anthropogenic Greenhouse Gas (GHG) emissions. As a result, a technology that can offset GHG emissions associated with buildings has the potential to save over 9 Giga-tons of GHG emissions per year. High temperature fuel cell and absorption chiller (HTFC/AC) technology offers a relatively low-carbon option for meeting cooling and electric loads for buildings while producing almost no criteria pollutants. GHG emissions in the state of California would decrease by 7.48 million metric tons per year if every commercial building in the State used HTFC/AC technology to meet its power and cooling requirements. In order to realize the benefits of HTFC/AC technology on a wide scale, the distributed generation market needs to be exposed to the technology and informed of its economic viability and real-world potential. This work characterizes the economics associated with HTFC/AC technology using select scenarios that are representative of realistic applications. The financial impacts of various input factors are evaluated and the HTFC/AC simulations are compared to the economics of traditional building utilities. It is shown that, in addition to the emissions reductions derived from the systems, HTFC/AC technology is financially preferable in all of the scenarios evaluated. This work also presents the design of a showcase environment, centered on a beta-test application, that presents (1) system operating data gathered using a custom data acquisition module, and (2) HTFC/AC technology in a clear and approachable manner in order to serve the target audience of market stakeholders.
4. MISSILE TEST AND ASSEMBLY BUILDING, LEFT SIDE, LOOKING NORTH. ...
4. MISSILE TEST AND ASSEMBLY BUILDING, LEFT SIDE, LOOKING NORTH. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL
2. MISSILE TEST AND ASSEMBLY BUILDING, RIGHT SIDE, LOOKING WEST. ...
2. MISSILE TEST AND ASSEMBLY BUILDING, RIGHT SIDE, LOOKING WEST. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL
3. MISSILE TEST AND ASSEMBLY BUILDING, REAR SIDE, LOOKING NORTH. ...
3. MISSILE TEST AND ASSEMBLY BUILDING, REAR SIDE, LOOKING NORTH. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL
ENGINEERING TEST REACTOR, TRA642. CONTEXTUAL VIEW ORIENTATING ETR TO MTR. ...
ENGINEERING TEST REACTOR, TRA-642. CONTEXTUAL VIEW ORIENTATING ETR TO MTR. CAMERA IS ON ROOF OF MTR BUILDING AND FACES DUE SOUTH. MTR SERVICE BUILDING, TRA-635, IN LOWER RIGHT CORNER. STEEL FRAMES SHOW BUILDINGS TO BE ATTACHED TO ETR BUILDING. HIGH-BAY SECTION IN CENTER IS REACTOR BUILDING. TWO-STORY CONTROL ROOM AND OFFICE BUILDING, TRA-647, IS BETWEEN IT AND MTR SERVICE BUILDING. STRUCTURE TO THE LEFT (WITH NO FRAMING YET) IS COMPRESSOR BUILDING, TRA-643, AND BEYOND IT WILL BE HEAT EXCHANGER BUILDING, TRA-644, GREAT SOUTHERN BUTTE ON HORIZON. INL NEGATIVE NO. 56-2382. Jack L. Anderson, Photographer, 6/10/1956 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
Rakers, Sebastian; Klinger, Matthias; Kruse, Charli; Gebert, Marina
2011-12-01
Here, we report the establishment of a permanent skin cell culture from rainbow trout (Oncorhynchus mykiss). The cells of the fish skin cell culture could be propagated over 60 passages so far. Furthermore, we show for the first time that it is possible to integrate freshly harvested rainbow trout scales into this new fish skin cell culture. We further demonstrated that epithelial cells derived from the scales survived in the artificial micro-environment of surrounding fibroblast-like cells. Also, antibody staining indicated that both cell types proliferated and started to build connections with the other cell type. It seems that it is possible to generate an 'artificial skin' with two different cell types. This could lead to the development of a three-dimensional test system, which might be a better in vitro representative of fish skin in vivo than individual skin cell lines. Copyright © 2011 Elsevier GmbH. All rights reserved.
Applying the cell-based coagulation model in the management of critical bleeding.
Ho, K M; Pavey, W
2017-03-01
The cell-based coagulation model was proposed 15 years ago, yet has not been applied commonly in the management of critical bleeding. Nevertheless, this alternative model may better explain the physiological basis of current coagulation management during critical bleeding. In this article we describe the limitations of the traditional coagulation protein cascade and standard coagulation tests, and explain the potential advantages of applying the cell-based model in current coagulation management strategies. The cell-based coagulation model builds on the traditional coagulation model and explains many recent clinical observations and research findings related to critical bleeding unexplained by the traditional model, including the encouraging results of using empirical 1:1:1 fresh frozen plasma:platelets:red blood cells transfusion strategy, and the use of viscoelastic and platelet function tests in patients with critical bleeding. From a practical perspective, applying the cell-based coagulation model also explains why new direct oral anticoagulants are effective systemic anticoagulants even without affecting activated partial thromboplastin time or the International Normalized Ratio in a dose-related fashion. The cell-based coagulation model represents the most cohesive scientific framework on which we can understand and manage coagulation during critical bleeding.
5. MISSILE TEST AND ASSEMBLY BUILDING, FRONT AND RIGHT SIDES, ...
5. MISSILE TEST AND ASSEMBLY BUILDING, FRONT AND RIGHT SIDES, LOOKING SOUTHEAST. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL
6. MISSILE TEST AND ASSEMBLY BUILDING, REAR AND LEFT SIDES, ...
6. MISSILE TEST AND ASSEMBLY BUILDING, REAR AND LEFT SIDES, LOOKING NORTHWEST. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL
Magnetic Suspension for Dynamic Spin Rig
NASA Technical Reports Server (NTRS)
Johnson, Dexter
1998-01-01
NASA Lewis Research Center's Dynamic Spin Rig, located in Building 5, Test Cell CW-18, is used to test turbomachinery blades and components by rotating them in a vacuum chamber. A team from Lewis' Machine Dynamics Branch successfully integrated a magnetic bearing and control system into the Dynamic Spin Rig. The magnetic bearing worked very well both to support and shake the shaft. It was demonstrated that the magnetic bearing can transmit more vibrational energy into the shaft and excite some blade modes to larger amplitudes than the existing electromagnetic shakers can.
NASA Astrophysics Data System (ADS)
Pine, G. D.; Christian, J. E.; Mixon, W. R.; Jackson, W. L.
1980-07-01
The procedures and data sources used to develop an energy consumption and system cost data base for use in predicting the market penetration of phosphoric acid fuel cell total energy systems in the nonindustrial building market are described. A computer program was used to simulate the hourly energy requirements of six types of buildings; office buildings; retail stores; hotels and motels; schools; hospitals; and multifamily residences. The simulations were done by using hourly weather tapes for one city in each of the ten Department of Energy administrative regions. Two types of building construction were considered, one for existing buildings and one for new buildings. A fuel cell system combined with electrically driven heat pumps and one combined with a gas boiler and an electrically driven chiller were compared with similar conventional systems. The methods of system simulation, component sizing, and system cost estimation are described for each system.
NASA Technical Reports Server (NTRS)
Cody, Dennis J.; Concepcion, Allan G.; Watras, Edward C., III
1995-01-01
This project, conducted in cooperation with the NASA Advanced Space Design Program, is part of an ongoing effort to place an experiment package into space. The goal of this project is to build and test flight-ready hardware that can be launched from the Space Shuttle. Get Away Special Canister 2 (GASCan 2) consists of three separate experiments. The Ionospheric Properties and Propagation Experiment (IPPE) determines effects of the ionosphere on radio wave propagation. The Microgravity Ignition experiment (MGI) tests the effects of combustion in a microgravity environment. The Rotational Fluid Flow experiment (RFF) examines fluid behavior under varying levels of gravity. This year the following tasks were completed: design of the IPPE antenna, X- and J-cell battery boxes, J-cell battery box enclosure, and structural bumpers; construction of the MGI canisters, MGI mounting brackets, IPPE antenna, and battery boxes; and the selection of the RFF's operating fluid and the analysis of the fluid behavior under microgravity test conditions.
NASA Astrophysics Data System (ADS)
Nguyen, Gia Luong Huu
Fuel cells can produce electricity with high efficiency, low pollutants, and low noise. With the advent of fuel cell technologies, fuel cell systems have since been demonstrated as reliable power generators with power outputs from a few watts to a few megawatts. With proper equipment, fuel cell systems can produce heating and cooling, thus increased its overall efficiency. To increase the acceptance from electrical utilities and building owners, fuel cell systems must operate more dynamically and integrate well with renewable energy resources. This research studies the dynamic performance of fuel cells and the integration of fuel cells with other equipment in three levels: (i) the fuel cell stack operating on hydrogen and reformate gases, (ii) the fuel cell system consisting of a fuel reformer, a fuel cell stack, and a heat recovery unit, and (iii) the hybrid energy system consisting of photovoltaic panels, fuel cell system, and energy storage. In the first part, this research studied the steady-state and dynamic performance of a high temperature PEM fuel cell stack. Collaborators at Aalborg University (Aalborg, Denmark) conducted experiments on a high temperature PEM fuel cell short stack at steady-state and transients. Along with the experimental activities, this research developed a first-principles dynamic model of a fuel cell stack. The dynamic model developed in this research was compared to the experimental results when operating on different reformate concentrations. Finally, the dynamic performance of the fuel cell stack for a rapid increase and rapid decrease in power was evaluated. The dynamic model well predicted the performance of the well-performing cells in the experimental fuel cell stack. The second part of the research studied the dynamic response of a high temperature PEM fuel cell system consisting of a fuel reformer, a fuel cell stack, and a heat recovery unit with high thermal integration. After verifying the model performance with the obtained experimental data, the research studied the control of airflow to regulate the temperature of reactors within the fuel processor. The dynamic model provided a platform to test the dynamic response for different control gains. With sufficient sensing and appropriate control, a rapid response to maintain the temperature of the reactor despite an increase in power was possible. The third part of the research studied the use of a fuel cell in conjunction with photovoltaic panels, and energy storage to provide electricity for buildings. This research developed an optimization framework to determine the size of each device in the hybrid energy system to satisfy the electrical demands of buildings and yield the lowest cost. The advantage of having the fuel cell with photovoltaic and energy storage was the ability to operate the fuel cell at baseload at night, thus reducing the need for large battery systems to shift the solar power produced in the day to the night. In addition, the dispatchability of the fuel cell provided an extra degree of freedom necessary for unforeseen disturbances. An operation framework based on model predictive control showed that the method is suitable for optimizing the dispatch of the hybrid energy system.
MATERIALS TESTING REACTOR (MTR) BUILDING, TRA603. CONTEXTUAL VIEW OF MTR ...
MATERIALS TESTING REACTOR (MTR) BUILDING, TRA-603. CONTEXTUAL VIEW OF MTR BUILDING SHOWING NORTH SIDES OF THE HIGH-BAY REACTOR BUILDING, ITS SECOND/THIRD FLOOR BALCONY LEVEL, AND THE ATTACHED ONE-STORY OFFICE/LABORATORY BUILDING, TRA-604. CAMERA FACING SOUTHEAST. VERTICAL CONCRETE-SHROUDED BEAMS SUPPORT PRECAST CONCRETE PANELS. CONCRETE PROJECTION FORMED AS A BUNKER AT LEFT OF VIEW IS TRA-657, PLUG STORAGE BUILDING. INL NEGATIVE NO. HD46-42-1. Mike Crane, Photographer, 4/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
Development and testing of shingle-type solar cell modules. Final report
DOE Office of Scientific and Technical Information (OSTI.GOV)
Shepard, N.F.
1979-02-28
The design, development, fabrication and testing of a shingle-type terrestrial solar cell module which produces 98 watts/m/sup 2/ of exposed module area at 1 kW/m/sup 2/ insolation and 61/sup 0/C are reported. These modules make it possible to easily incorporate photovoltaic power generation into the sloping roofs of residential or commercial buildings by simply nailing the modules to the plywood roof sheathing. This design consists of nineteen series-connected 53 mm diameter solar cells arranged in a closely packaged hexagon configuration. These cells are individually bonded to the embossed surface of a 3 mm thick thermally tempered hexagon-shaped piece of ASGmore » SUNADEX glass. Monsanto SAFLEX polyvinyl butyral is used as the laminating adhesive. RTVII functions as the encapsulant between the underside of the glass superstrate and a rear protective sheet of 0.8 mm thick TEXTOLITE. The semi-flexible portion of each shingle module is a composite laminate construction consisting of outer layers of B.F. Goodrich FLEXSEAL and an epichlorohydrin closed cell foam core. The module design has satisfactorily survived the JPL-defined qualification testing program which includes 50 thermal cycles between -40 and +90/sup 0/C, a seven-day temperature-humidity exposure test and a mechanical integrity test consisting of a bidirectional cyclic loading at 2390 Pa (50 lb/ft/sup 2/) which is intended to simulate loads due to a 45 m/s (100 mph) wind.« less
Project Plan 7930 Cell G PaR Remote Handling System Replacement
DOE Office of Scientific and Technical Information (OSTI.GOV)
Kinney, Kathryn A
2009-10-01
For over 40 years the US Department of Energy (DOE) and its predecessors have made Californium-252 ({sup 252}Cf) available for a wide range of industries including medical, nuclear fuels, mining, military and national security. The Radiochemical Engineering Development Center (REDC) located within the Oak Ridge National Laboratory (ORNL) processes irradiated production targets from the High Flux Isotope Reactor (HFIR). Operations in Building 7930, Cell G provide over 70% of the world's demand for {sup 252}Cf. Building 7930 was constructed and equipped in the mid-1960s. Current operations for {sup 252}Cf processing in Building 7930, Cell G require use of through-the-wall manipulatorsmore » and the PaR Remote Handling System. Maintenance and repairs for the manipulators is readily accomplished by removal of the manipulator and relocation to a repair shop where hands-on work can be performed in glove boxes. Contamination inside cell G does not currently allow manned entry and no provisions were created for a maintenance area inside the cell. There has been no maintenance of the PaR system or upgrades, leaving operations vulnerable should the system have a catastrophic failure. The Cell G PaR system is currently being operated in a run to failure mode. As the manipulator is now 40+ years old there is significant risk in this method of operation. In 2006 an assessment was completed that resulted in recommendations for replacing the manipulator operator control and power centers which are used to control and power the PaR manipulator in Cell G. In mid-2008 the chain for the bridge drive failed and subsequent examinations indicated several damaged links (see Figure 1). To continue operations the PaR manipulator arm is being used to push and pull the bridge as a workaround. A retrieval tool was fabricated, tested and staged inside Cell G that will allow positioning of the bridge and manipulator arm for removal from the cell should the PaR system completely fail. A fully functioning and reliable Par manipulator arm is necessary for uninterrupted {sup 252}Cf operations; a fully-functioning bridge is needed for the system to function as intended.« less
Adsorption of IgG on/in a PAH/PSS multilayer film: Layer structure and cell response.
Feldötö, Zsombor; Lundin, Maria; Braesch-Andersen, Sten; Blomberg, Eva
2011-02-01
The binding of immunogloblulins (IgG) (mouse monoclonal recognizing IFNγ) on precoated polystyrene or silica surfaces by the layer-by-layer technique has been investigated with QCM-D and DPI. The aim of the work was to increase the sensitivity of the conventional enzyme-linked immunosorbent spot (ELISpot) assay. The polyelectrolytes used to build the multilayers were poly(allylamine hydrochloride) (PAH)/poly(sodium 4-styrenesulfonate) (PSS) alternately adsorbed from 150mM NaCl. The multilayer build up is linear and the internal structure of the PAH/PSS multilayer is compact and rigid as observed by low relative water content (20-25%) and high layer refractive index (n∼1.5) after the formation of five bilayers. Incorporation of IgG within the PAH/PSS multilayer did not give rise to overcharging and did not affect the linear build up. ELISpot test on PAH/PSS multilayer modified polystyrene wells showed that the cytokine response was significantly smaller than on the regular PVDF backed polystyrene wells. This may be due to the compact and rigid nature of the PAH/PSS multilayer, which does not allow formation of the kind of three dimensional support needed to achieve bioactive IgG binding to the surface. Immunological tests of the polyelectrolyte multilayers in the absence of IgG showed that PSS terminated PAH/PSS multilayer did not induce any cytokine response whereas PAH terminated did, which suggests that PSS totally covers the surface from the cells point of view. Copyright © 2010 Elsevier Inc. All rights reserved.
Shaking table test and dynamic response prediction on an earthquake-damaged RC building
NASA Astrophysics Data System (ADS)
Xianguo, Ye; Jiaru, Qian; Kangning, Li
2004-12-01
This paper presents the results from shaking table tests of a one-tenth-scale reinforced concrete (RC) building model. The test model is a protype of a building that was seriously damaged during the 1985 Mexico earthquake. The input ground excitation used during the test was from the records obtained near the site of the prototype building during the 1985 and 1995 Mexico earthquakes. The tests showed that the damage pattern of the test model agreed well with that of the prototype building. Analytical prediction of earthquake response has been conducted for the prototype building using a sophisticated 3-D frame model. The input motion used for the dynamic analysis was the shaking table test measurements with similarity transformation. The comparison of the analytical results and the shaking table test results indicates that the response of the RC building to minor and the moderate earthquakes can be predicated well. However, there is difference between the predication and the actual response to the major earthquake.
In-house fabrication and testing capabilities for Li and Li-ion 18650 cells
NASA Astrophysics Data System (ADS)
Nagasubramanian, G.
2010-04-01
For over 10 years Sandia Labs have been involved in an US DOE-funded program aimed at developing electric vehicle batteries for transportation applications. Currently this program is called "Advanced Battery Research (ABR)." In this effort we were preparing 18650 cells with electrodes supplied by or purchased from private companies for thermal abuse and electrical characterization studies. Lately, we are coating our own electrodes, building cells and evaluating performance. This paper describes our extensive in-house facilities for slurry making, electrode coating, cell winding etc. In addition, facilities for electrical testing and thermal abuse will be described. This facility allows us to readjust our focus quickly to the changing demands of the still evolving ABR program. Additionally, we continue to make cells for our internal use. We made several 18650 cells both primary (Li-CFx) and secondary (Li-ion) and evaluated performance. For example Li-CFx cells gave ~2.9Ahr capacity at room temperature. Our high voltage Li-ion cells consisting of carbon anode and cathode based on LiNi 0.4Mn 0.3Co 0.3O2 in organic electrolytes exhibited reproducible behavior and gave capacity on the order of 1Ahr. Performance of Li-ion cells at different temperatures and thermal abuse characteristics will be presented.
Rapid Prototyping of Microbial Cell Factories via Genome-scale Engineering
Si, Tong; Xiao, Han; Zhao, Huimin
2014-01-01
Advances in reading, writing and editing genetic materials have greatly expanded our ability to reprogram biological systems at the resolution of a single nucleotide and on the scale of a whole genome. Such capacity has greatly accelerated the cycles of design, build and test to engineer microbes for efficient synthesis of fuels, chemicals and drugs. In this review, we summarize the emerging technologies that have been applied, or are potentially useful for genome-scale engineering in microbial systems. We will focus on the development of high-throughput methodologies, which may accelerate the prototyping of microbial cell factories. PMID:25450192
Solar Cells Light Up Prison Cells on 'The Rock' | News | NREL
2 » Solar Cells Light Up Prison Cells on 'The Rock' Solar Cells Light Up Prison Cells on 'The Rock ' July 23, 2012 This photo shows an island in the middle of blue sea water, with industrial buildings taking up a good deal of the island. The 1,300 solar panels on the Cellhouse building are a dark blue
11. VIEW OF INTERIOR OF BUILDING 220 FIRST FLOOR, CELL ...
11. VIEW OF INTERIOR OF BUILDING 220 FIRST FLOOR, CELL BLOCK 'A' (SOLITARY CONFINEMENT CELL BLOCK), TYPICAL SOLITARY CONFINEMENT CELL. THE CELL SHOWN IN CENTER OF PHOTO, HAS A 2-1/2' THICK STEEL DOOR. THE CELL SHOWN IN THE LEFT OF PHOTO, HAS A 3/4' DIAMETER IRON GRILLE DOOR. - U.S. Naval Base, Pearl Harbor, Brig, Neville Way near Ninth Street at Marine Barracks, Pearl City, Honolulu County, HI
2010-01-06
CAPE CANAVERAL, Fla. – In the transfer aisle of the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, the external fuel tank for space shuttle Discovery's STS-131 mission, ET-135, is lifted from its transporter toward a test cell. The tank was delivered to Kennedy aboard the Pegasus barge from NASA's Michoud Assembly Facility on Dec. 26. The tank will remain in the test cell until it is transferred into a high bay for mating with the twin solid rocket boosters that will be used on the mission. Launch of the STS-131 mission to the International Space Station is targeted for March 18. For information on the STS-131 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts131/index.html. Photo credit: NASA/Glenn Benson
2010-01-06
CAPE CANAVERAL, Fla. – In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, workers prepare the external fuel tank for space shuttle Discovery's STS-131 mission, ET-135, hanging vertically in the transfer aisle, for its lift into a test cell. The tank was delivered to Kennedy aboard the Pegasus barge from NASA's Michoud Assembly Facility on Dec. 26. The tank will remain in the test cell until it is transferred into a high bay for mating with the twin solid rocket boosters that will be used on the mission. Launch of the STS-131 mission to the International Space Station is targeted for March 18. For information on the STS-131 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts131/index.html. Photo credit: NASA/Glenn Benson
2010-01-06
CAPE CANAVERAL, Fla. – In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, workers prepare to lift the external fuel tank for space shuttle Discovery's STS-131 mission, ET-135, from the transfer aisle into a test cell. The tank was delivered to Kennedy aboard the Pegasus barge from NASA's Michoud Assembly Facility on Dec. 26. The tank will remain in the test cell until it is transferred into a high bay for mating with the twin solid rocket boosters that will be used on the mission. Launch of the STS-131 mission to the International Space Station is targeted for March 18. For information on the STS-131 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts131/index.html. Photo credit: NASA/Glenn Benson
2010-01-06
CAPE CANAVERAL, Fla. – In the transfer aisle of the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, a crane lifts the external fuel tank for space shuttle Discovery's STS-131 mission, ET-135, from its transporter toward a test cell. The tank was delivered to Kennedy aboard the Pegasus barge from NASA's Michoud Assembly Facility on Dec. 26. The tank will remain in the test cell until it is transferred into a high bay for mating with the twin solid rocket boosters that will be used on the mission. Launch of the STS-131 mission to the International Space Station is targeted for March 18. For information on the STS-131 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts131/index.html. Photo credit: NASA/Glenn Benson
2010-01-06
CAPE CANAVERAL, Fla. – In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, the external fuel tank for space shuttle Discovery's STS-131 mission, ET-135, is lifted above the transfer aisle for transfer into a test cell. The tank was delivered to Kennedy aboard the Pegasus barge from NASA's Michoud Assembly Facility on Dec. 26. The tank will remain in the test cell until it is transferred into a high bay for mating with the twin solid rocket boosters that will be used on the mission. Launch of the STS-131 mission to the International Space Station is targeted for March 18. For information on the STS-131 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts131/index.html. Photo credit: NASA/Glenn Benson
2010-01-06
CAPE CANAVERAL, Fla. – In the transfer aisle of the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, the external fuel tank for space shuttle Discovery's STS-131 mission, ET-135, is rotated into a vertical position as it is lifted toward a test cell. The tank was delivered to Kennedy aboard the Pegasus barge from NASA's Michoud Assembly Facility on Dec. 26. The tank will remain in the test cell until it is transferred into a high bay for mating with the twin solid rocket boosters that will be used on the mission. Launch of the STS-131 mission to the International Space Station is targeted for March 18. For information on the STS-131 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts131/index.html. Photo credit: NASA/Glenn Benson
2010-01-06
CAPE CANAVERAL, Fla. – In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, workers are on hand to monitor the external fuel tank for space shuttle Discovery's STS-131 mission, ET-135, as it is positioned into a test cell. The tank was delivered to Kennedy aboard the Pegasus barge from NASA's Michoud Assembly Facility on Dec. 26. The tank will remain in the test cell until it is transferred into a high bay for mating with the twin solid rocket boosters that will be used on the mission. Launch of the STS-131 mission to the International Space Station is targeted for March 18. For information on the STS-131 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts131/index.html. Photo credit: NASA/Glenn Benson
NASA Astrophysics Data System (ADS)
Liu, Xiaoyu; Allen, Matthew R.; Roache, Nancy F.
2016-09-01
Better understanding the transport mechanisms of organophosphorus flame-retardants (OPFRs) in the residential environment is important to more accurately estimate their indoor exposure and develop risk management strategies that protect human health. This study describes an improved dual small chamber testing method to characterize the sorption of OPFRs on indoor building materials and consumer products. The OPFRs studied were tris(2-chloroethyl) phosphate (TCEP), tris(1-chloro-2-propyl) phosphate (TCIPP), and tris(1,3-dichloro-2-propyl) phosphate (TDCIPP). The test materials and products used as sinks include concrete, ceiling tile, vinyl flooring, carpet, latex painted gypsum wallboard, open cell polyurethane foam, mattress pad and liner, polyester clothing, cotton clothing, and uniform shirt. During the tests, the amount of OPFRs absorbed by the materials at different exposure times was determined simultaneously. OPFRs air concentrations at the inlet and inside the test chamber were monitored. The data were used to rank the sorption strength of the OPFRs on different materials. In general, building materials exhibited relatively stronger sorption strength than clothing textiles. The material-air partition and material phase diffusion coefficients were estimated by fitting a sink model to the sorption concentration data for twelve materials with three OPFRs. They are in the range of 2.72 × 105 to 3.99 × 108 (dimensionless) for the material-air partition coefficients and 1.13 × 10-14 to 5.83 × 10-9 (m2/h) for the material phase diffusion coefficients.
FLOOR PLAN OF MAIN PROCESSING BUILDING (CPP601), SECOND FLOOR SHOWING ...
FLOOR PLAN OF MAIN PROCESSING BUILDING (CPP-601), SECOND FLOOR SHOWING PROCESS MAKEUP AREA AND EIGHTEEN CELLS AND ADJOINING REMOTE ANALYTICAL FACILITY (CPP-627) SHOWING COLD LAB, DECONTAMINATION ROOM, MULTICURIE CELL ROOM, AND OFFICES. TO LEFT ARE LABORATORY BUILDING (CPP-602) AND MAINTENANCE BUILDING (CPP-630). INL DRAWING NUMBER 200-0601-00-706-051980. ALTERNATE ID NUMBER CPP-E-1980. - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID
FLOOR PLAN OF MAIN PROCESSING BUILDING (CPP601), FIRST FLOOR SHOWING ...
FLOOR PLAN OF MAIN PROCESSING BUILDING (CPP-601), FIRST FLOOR SHOWING SAMPLE CORRIDORS AND EIGHTEEN CELLS AND ADJOINING REMOTE ANALYTICAL FACILITY (CPP-627) SHOWING REMOTE ANALYTICAL FACILITIES LAB, DECONTAMINATION ROOM, AND MULTICURIE CELL ROOM. TO LEFT ARE LABORATORY BUILDING (CPP-602) AND MAINTENANCE BUILDING (CPP-630). INL DRAWING NUMBER 200-0601-00-706-051979. ALTERNATE ID NUMBER CPP-E-1979. - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID
Kujundzic, Elmira; Zander, David A; Hernandez, Mark; Angenent, Largus T; Henderson, David E; Miller, Shelly L
2005-02-01
The purpose of this study was to assess the effectiveness of a new generation of high-volume, ceiling-mounted high-efficiency particulate air (HEPA)-ultraviolet (UV) air filters (HUVAFs) for their ability to remove or inactivate bacterial aerosol. In an environmentally controlled full-scale laboratory chamber (87 m3), and an indoor therapy pool building, the mitigation ability of air filters was assessed by comparing concentrations of total bacteria, culturable bacteria, and airborne endotoxin with and without the air filters operating under otherwise similar conditions. Controlled chamber tests with pure cultures of aerosolized Mycobacterium parafortuitum cells showed that the HUVAF unit tested provided an equivalent air-exchange rate of 11 hr(-1). Using this equivalent air-exchange rate as a design basis, three HUVAFs were installed in an indoor therapy pool building for bioaerosol mitigation, and their effectiveness was studied over a 2-year period. The HUVAFs reduced concentrations of culturable bacteria by 69 and 80% during monitoring periods executed in respective years. The HUVAFs reduced concentrations of total bacteria by 12 and 76% during the same monitoring period, respectively. Airborne endotoxin concentrations were not affected by the HUVAF operation.
Modeling and Optimization of Commercial Buildings and Stationary Fuel Cell Systems (Presentation)
DOE Office of Scientific and Technical Information (OSTI.GOV)
Ainscough, C.; McLarty, D.; Sullivan, R.
2013-10-01
This presentation describes the Distributed Generation Building Energy Assessment Tool (DG-BEAT) developed by the National Renewable Energy Laboratory and the University of California Irvine. DG-BEAT is designed to allow stakeholders to assess the economics of installing stationary fuel cell systems in a variety of building types in the United States.
3. TEST AREA 1115, OVERVIEW. AT RIGHT IS BUILDING 8647, ...
3. TEST AREA 1-115, OVERVIEW. AT RIGHT IS BUILDING 8647, TEST STAND 1-4. AT LEFT CENTER, IN THE MIDDLE DISTANCE, IS BUILDING 8668, INSTRUMENTATION AND CONTROL BUILDING FOR TEST AREA 1-115. Looking east from the deck of Test Stand 1-5. (PANORAMA 2/2) - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Leuhman Ridge near Highways 58 & 395, Boron, Kern County, CA
NASA Astrophysics Data System (ADS)
Han, Jinhyup; Hwang, Soo Min; Go, Wooseok; Senthilkumar, S. T.; Jeon, Donghoon; Kim, Youngsik
2018-01-01
Cell design and optimization of the components, including active materials and passive components, play an important role in constructing robust, high-performance rechargeable batteries. Seawater batteries, which utilize earth-abundant and natural seawater as the active material in an open-structured cathode, require a new platform for building and testing the cells other than typical Li-ion coin-type or pouch-type cells. Herein, we present new findings based on our optimized cell. Engineering the cathode components-improving the wettability of cathode current collector and seawater catholyte flow-improves the battery performance (voltage efficiency). Optimizing the cell component and design is the key to identifying the electrochemical processes and reactions of active materials. Hence, the outcome of this research can provide a systematic study of potentially active materials used in seawater batteries and their effectiveness on the electrochemical performance.
New decision support tool for acute lymphoblastic leukemia classification
NASA Astrophysics Data System (ADS)
Madhukar, Monica; Agaian, Sos; Chronopoulos, Anthony T.
2012-03-01
In this paper, we build up a new decision support tool to improve treatment intensity choice in childhood ALL. The developed system includes different methods to accurately measure furthermore cell properties in microscope blood film images. The blood images are exposed to series of pre-processing steps which include color correlation, and contrast enhancement. By performing K-means clustering on the resultant images, the nuclei of the cells under consideration are obtained. Shape features and texture features are then extracted for classification. The system is further tested on the classification of spectra measured from the cell nuclei in blood samples in order to distinguish normal cells from those affected by Acute Lymphoblastic Leukemia. The results show that the proposed system robustly segments and classifies acute lymphoblastic leukemia based on complete microscopic blood images.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Hasegawa, H.K.; Staggs, K.J.; Doughty, S.M.
1992-12-01
As a result of a DOE (Tiger Team) Technical Safety Appraisal (November 1990) of the Radiochemical Engineering Development Center (REDC), ORNL Building 7920, a number of fire protection concerns were identified. The primary concern was the perceived loss of ventilation system containment due to the thermal destruction and/or breaching of the prefilters and/or high-efficiency particulate air filters (HEPA `s) and the resultant radioactive release to the external environment. The following report describes the results of an extensive fire test program performed by the Fire Research Discipline (FRD) of the Special Projects Division of Lawrence Livermore National Lab (LLNL) and fundedmore » by ORNL to address these concerns. Full scale mock-ups of a REDC hot cell tank pit, adjacent cubicle pit, and associated ventilation system were constructed at LLNL and 13 fire experiments were conducted to specifically answer the questions raised by the Tiger Team. Our primary test plan was to characterize the burning of a catastrophic solvent spill (kerosene) of 40 liters and its effect on the containment ventilation system prefilters and HEPA filters. In conjunction with ORNL and Lockwood Greene we developed a test matrix that assessed the fire performance of the prefilters and HEPA filters; evaluated the fire response of the fiber reinforced plastic (FRP) epoxy ventilation duct work; the response and effectiveness of the fire protection system, the effect of fire in a cubicle on the vessel off-gas (VOG) elbow, and other fire safety questions.« less
Thermal Cycling of Mir Cooperative Solar Array (MCSA) Test Panels
NASA Technical Reports Server (NTRS)
Hoffman, David J.; Scheiman, David A.
1997-01-01
The Mir Cooperative Solar Array (MCSA) project was a joint US/Russian effort to build a photovoltaic (PV) solar array and deliver it to the Russian space station Mir. The MCSA is currently being used to increase the electrical power on Mir and provide PV array performance data in support of Phase 1 of the International Space Station (ISS), which will use arrays based on the same solar cells used in the MCSA. The US supplied the photovoltaic power modules (PPMs) and provided technical and programmatic oversight while Russia provided the array support structures and deployment mechanism and built and tested the array. In order to ensure that there would be no problems with the interface between US and Russian hardware, an accelerated thermal life cycle test was performed at NASA Lewis Research Center on two representative samples of the MCSA. Over an eight-month period (August 1994 - March 1995), two 15-cell MCSA solar array 'mini' panel test articles were simultaneously put through 24,000 thermal cycles (+80 C to -100 C), equivalent to four years on-orbit. The test objectives, facility, procedure and results are described in this paper. Post-test inspection and evaluation revealed no significant degradation in the structural integrity of the test articles and no electrical degradation, not including one cell damaged early as an artifact of the test and removed from consideration. The interesting nature of the performance degradation caused by this one cell, which only occurred at elevated temperatures, is discussed. As a result of this test, changes were made to improve some aspects of the solar cell coupon-to-support frame interface on the flight unit. It was concluded from the results that the integration of the US solar cell modules with the Russian support structure would be able to withstand at least 24,000 thermal cycles (4 years on-orbit).
Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology.
Jusiak, Barbara; Cleto, Sara; Perez-Piñera, Pablo; Lu, Timothy K
2016-07-01
One of the goals of synthetic biology is to build regulatory circuits that control cell behavior, for both basic research purposes and biomedical applications. The ability to build transcriptional regulatory devices depends on the availability of programmable, sequence-specific, and effective synthetic transcription factors (TFs). The prokaryotic clustered regularly interspaced short palindromic repeat (CRISPR) system, recently harnessed for transcriptional regulation in various heterologous host cells, offers unprecedented ease in designing synthetic TFs. We review how CRISPR can be used to build synthetic gene circuits and discuss recent advances in CRISPR-mediated gene regulation that offer the potential to build increasingly complex, programmable, and efficient gene circuits in the future. Copyright © 2016. Published by Elsevier Ltd.
Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities.
Casey, Alison E; Sinha, Ankit; Singhania, Rajat; Livingstone, Julie; Waterhouse, Paul; Tharmapalan, Pirashaanthy; Cruickshank, Jennifer; Shehata, Mona; Drysdale, Erik; Fang, Hui; Kim, Hyeyeon; Isserlin, Ruth; Bailey, Swneke; Medina, Tiago; Deblois, Genevieve; Shiah, Yu-Jia; Barsyte-Lovejoy, Dalia; Hofer, Stefan; Bader, Gary; Lupien, Mathieu; Arrowsmith, Cheryl; Knapp, Stefan; De Carvalho, Daniel; Berman, Hal; Boutros, Paul C; Kislinger, Thomas; Khokha, Rama
2018-06-19
The mammary epithelium depends on specific lineages and their stem and progenitor function to accommodate hormone-triggered physiological demands in the adult female. Perturbations of these lineages underpin breast cancer risk, yet our understanding of normal mammary cell composition is incomplete. Here, we build a multimodal resource for the adult gland through comprehensive profiling of primary cell epigenomes, transcriptomes, and proteomes. We define systems-level relationships between chromatin-DNA-RNA-protein states, identify lineage-specific DNA methylation of transcription factor binding sites, and pinpoint proteins underlying progesterone responsiveness. Comparative proteomics of estrogen and progesterone receptor-positive and -negative cell populations, extensive target validation, and drug testing lead to discovery of stem and progenitor cell vulnerabilities. Top epigenetic drugs exert cytostatic effects; prevent adult mammary cell expansion, clonogenicity, and mammopoiesis; and deplete stem cell frequency. Select drugs also abrogate human breast progenitor cell activity in normal and high-risk patient samples. This integrative computational and functional study provides fundamental insight into mammary lineage and stem cell biology. © 2018 Casey et al.
Delivery of Human Adipose Stem Cells Spheroids into Lockyballs.
Silva, Karina R; Rezende, Rodrigo A; Pereira, Frederico D A S; Gruber, Peter; Stuart, Mellannie P; Ovsianikov, Aleksandr; Brakke, Ken; Kasyanov, Vladimir; da Silva, Jorge V L; Granjeiro, José M; Baptista, Leandra S; Mironov, Vladimir
2016-01-01
Adipose stem cells (ASCs) spheroids show enhanced regenerative effects compared to single cells. Also, spheroids have been recently introduced as building blocks in directed self-assembly strategy. Recent efforts aim to improve long-term cell retention and integration by the use of microencapsulation delivery systems that can rapidly integrate in the implantation site. Interlockable solid synthetic microscaffolds, so called lockyballs, were recently designed with hooks and loops to enhance cell retention and integration at the implantation site as well as to support spheroids aggregation after transplantation. Here we present an efficient methodology for human ASCs spheroids biofabrication and lockyballs cellularization using micro-molded non-adhesive agarose hydrogel. Lockyballs were produced using two-photon polymerization with an estimated mechanical strength. The Young's modulus was calculated at level 0.1362 +/-0.009 MPa. Interlocking in vitro test demonstrates high level of loading induced interlockability of fabricated lockyballs. Diameter measurements and elongation coefficient calculation revealed that human ASCs spheroids biofabricated in resections of micro-molded non-adhesive hydrogel had a more regular size distribution and shape than spheroids biofabricated in hanging drops. Cellularization of lockyballs using human ASCs spheroids did not alter the level of cells viability (p › 0,999) and gene fold expression for SOX-9 and RUNX2 (p › 0,195). The biofabrication of ASCs spheroids into lockyballs represents an innovative strategy in regenerative medicine, which combines solid scaffold-based and directed self-assembly approaches, fostering opportunities for rapid in situ biofabrication of 3D building-blocks.
51. CONTEXT VIEW LOOKING NORTHWEST OF BUILDING 365 (ARMAMENT TESTING ...
51. CONTEXT VIEW LOOKING NORTHWEST OF BUILDING 365 (ARMAMENT TESTING BUILDING) IN BASE SPARES AREA WITH BUILDING 367 (ADMINISTRATIVE OFFICE BUILDING) IN MIDDLE GROUND AND BUILDING 368 (WAREHOUSE) IN BACKGROUND. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME
FAST CHOPPER BUILDING, TRA665. CONTEXTUAL VIEW: CHOPPER BUILDING IN CENTER. ...
FAST CHOPPER BUILDING, TRA-665. CONTEXTUAL VIEW: CHOPPER BUILDING IN CENTER. MTR REACTOR SERVICES BUILDING,TRA-635, TO LEFT; MTR BUILDING TO RIGHT. CAMERA FACING WEST. INL NEGATIVE NO. HD42-1. Mike Crane, Photographer, 3/2004 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
An Update on the Lithium-Ion Cell Low-Earth-Orbit Verification Test Program
NASA Technical Reports Server (NTRS)
Reid, Concha M.; Manzo, Michelle A.; Miller, Thomas B.; McKissock, Barbara I.; Bennett, William
2007-01-01
A Lithium-Ion Cell Low-Earth-Orbit Verification Test Program is being conducted by NASA Glenn Research Center to assess the performance of lithium-ion (Li-ion) cells over a wide range of low-Earth-orbit (LEO) conditions. The data generated will be used to build an empirical model for Li-ion batteries. The goal of the modeling will be to develop a tool to predict the performance and cycle life of Li-ion batteries operating at a specified set of mission conditions. Using this tool, mission planners will be able to design operation points of the battery system while factoring in mission requirements and the expected life and performance of the batteries. Test conditions for the program were selected via a statistical design of experiments to span a range of feasible operational conditions for LEO aerospace applications. The variables under evaluation are temperature, depth-of-discharge (DOD), and end-of-charge voltage (EOCV). The baseline matrix was formed by generating combinations from a set of three values for each variable. Temperature values are 10 C, 20 C and 30 C. Depth-of-discharge values are 20%, 30% and 40%. EOCV values are 3.85 V, 3.95 V, and 4.05 V. Test conditions for individual cells may vary slightly from the baseline test matrix depending upon the cell manufacturer s recommended operating conditions. Cells from each vendor are being evaluated at each of ten sets of test conditions. Cells from four cell manufacturers are undergoing life cycle tests. Life cycling on the first sets of cells began in September 2004. These cells consist of Saft 40 ampere-hour (Ah) cells and Lith ion 30 Ah cells. These cells have achieved over 10,000 cycles each, equivalent to about 20 months in LEO. In the past year, the test program has expanded to include the evaluation of Mine Safety Appliances (MSA) 50 Ah cells and ABSL battery modules. The MSA cells will begin life cycling in October 2006. The ABSL battery modules consist of commercial Sony hard carbon 18650 lithium-ion cells configured in series and parallel combinations to create nominal 14.4 volt, 3 Ah packs (4s-2p). These modules have accumulated approximately 3000 cycles. Results on the performance of the cells and modules will be presented in this paper. The life prediction and performance model for Li-ion cells in LEO will be built by analyzing the data statistically and performing regression analysis. Cells are being cycled to failure so that differences in performance trends that occur at different stages in the life of the cell can be observed and accurately modeled. Cell testing is being performed at the Naval Surface Warfare Center in Crane, IN.
Accurately tracking single-cell movement trajectories in microfluidic cell sorting devices.
Jeong, Jenny; Frohberg, Nicholas J; Zhou, Enlu; Sulchek, Todd; Qiu, Peng
2018-01-01
Microfluidics are routinely used to study cellular properties, including the efficient quantification of single-cell biomechanics and label-free cell sorting based on the biomechanical properties, such as elasticity, viscosity, stiffness, and adhesion. Both quantification and sorting applications require optimal design of the microfluidic devices and mathematical modeling of the interactions between cells, fluid, and the channel of the device. As a first step toward building such a mathematical model, we collected video recordings of cells moving through a ridged microfluidic channel designed to compress and redirect cells according to cell biomechanics. We developed an efficient algorithm that automatically and accurately tracked the cell trajectories in the recordings. We tested the algorithm on recordings of cells with different stiffness, and showed the correlation between cell stiffness and the tracked trajectories. Moreover, the tracking algorithm successfully picked up subtle differences of cell motion when passing through consecutive ridges. The algorithm for accurately tracking cell trajectories paves the way for future efforts of modeling the flow, forces, and dynamics of cell properties in microfluidics applications.
Improving Building Energy Simulation Programs Through Diagnostic Testing (Fact Sheet)
DOE Office of Scientific and Technical Information (OSTI.GOV)
Not Available
2012-02-01
New test procedure evaluates quality and accuracy of energy analysis tools for the residential building retrofit market. Reducing the energy use of existing homes in the United States offers significant energy-saving opportunities, which can be identified through building simulation software tools that calculate optimal packages of efficiency measures. To improve the accuracy of energy analysis for residential buildings, the National Renewable Energy Laboratory's (NREL) Buildings Research team developed the Building Energy Simulation Test for Existing Homes (BESTEST-EX), a method for diagnosing and correcting errors in building energy audit software and calibration procedures. BESTEST-EX consists of building physics and utility billmore » calibration test cases, which software developers can use to compare their tools simulation findings to reference results generated with state-of-the-art simulation tools. Overall, the BESTEST-EX methodology: (1) Tests software predictions of retrofit energy savings in existing homes; (2) Ensures building physics calculations and utility bill calibration procedures perform to a minimum standard; and (3) Quantifies impacts of uncertainties in input audit data and occupant behavior. BESTEST-EX is helping software developers identify and correct bugs in their software, as well as develop and test utility bill calibration procedures.« less
View showing base of Building 70021 with Building 70022 in ...
View showing base of Building 70021 with Building 70022 in background, facing southeast - Naval Ordnance Test Station Inyokern, Randsburg Wash Facility Target Test Towers, Tower Road, China Lake, Kern County, CA
Detail view of base of Building 70021, showing Building 70022 ...
Detail view of base of Building 70021, showing Building 70022 (background), facing southeast - Naval Ordnance Test Station Inyokern, Randsburg Wash Facility Target Test Towers, Tower Road, China Lake, Kern County, CA
ENGINEERING TEST REACTOR (ETR) BUILDING, TRA642. CONTEXTUAL VIEW, CAMERA FACING ...
ENGINEERING TEST REACTOR (ETR) BUILDING, TRA-642. CONTEXTUAL VIEW, CAMERA FACING EAST. VERTICAL METAL SIDING. ROOF IS SLIGHTLY ELEVATED AT CENTER LINE FOR DRAINAGE. WEST SIDE OF ETR COMPRESSOR BUILDING, TRA-643, PROJECTS TOWARD LEFT AT FAR END OF ETR BUILDING. INL NEGATIVE NO. HD46-37-1. Mike Crane, Photographer, 4/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
21. Building 202, underside of test stand A, detail of ...
21. Building 202, underside of test stand A, detail of junction of scrubber structure and test stand with water pipes and valves visible. View looking southeast. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
Karunasena, Enusha; Larrañaga, Michael D; Simoni, Jan S; Douglas, David R; Straus, David C
2010-12-01
Damage to human neurological system cells resulting from exposure to mycotoxins confirms a previously controversial public health threat for occupants of water-damaged buildings. Leading scientific organizations disagree about the ability of inhaled mycotoxins in the indoor environment to cause adverse human health effects. Damage to the neurological system can result from exposure to trichothecene mycotoxins in the indoor environment. This study demonstrates that neurological system cell damage can occur from satratoxin H exposure to neurological cells at exposure levels that can be found in water-damaged buildings contaminated with fungal growth. The constant activation of inflammatory and apoptotic pathways at low levels of exposure in human brain capillary endothelial cells, astrocytes, and neural progenitor cells may amplify devastation to neurological tissues and lead to neurological system cell damage from indirect events triggered by the presence of trichothecenes.
SPERTI, Instrument Cell Building (PER606). North facade. Date: August 2003. ...
SPERT-I, Instrument Cell Building (PER-606). North facade. Date: August 2003. INEEL negative no. HD-35-3-3 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID
View of the southwest guard tower, cell blocks seven and ...
View of the southwest guard tower, cell blocks seven and eight, administration building west tower, and Fairmount Avenue, looking from the administration building facing west - Eastern State Penitentiary, 2125 Fairmount Avenue, Philadelphia, Philadelphia County, PA
SPERTI. Detail view of Reactor Pit Building (PER605) and Instrument ...
SPERT-I. Detail view of Reactor Pit Building (PER-605) and Instrument Cell (PER-606). Earth shielding covers side of Cell Building next to reactor. Instrumentation required protection from radiation emitted during reactor operation. Photographer: R.G. Larsen. Date: May 20, 1955. INEEL negative no. 55-1290 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID
Feasibility of solid oxide fuel cell dynamic hydrogen coproduction to meet building demand
NASA Astrophysics Data System (ADS)
Shaffer, Brendan; Brouwer, Jacob
2014-02-01
A dynamic internal reforming-solid oxide fuel cell system model is developed and used to simulate the coproduction of electricity and hydrogen while meeting the measured dynamic load of a typical southern California commercial building. The simulated direct internal reforming-solid oxide fuel cell (DIR-SOFC) system is controlled to become an electrical load following device that well follows the measured building load data (3-s resolution). The feasibility of the DIR-SOFC system to meet the dynamic building demand while co-producing hydrogen is demonstrated. The resulting thermal responses of the system to the electrical load dynamics as well as those dynamics associated with the filling of a hydrogen collection tank are investigated. The DIR-SOFC system model also allows for resolution of the fuel cell species and temperature distributions during these dynamics since thermal gradients are a concern for DIR-SOFC.
View of hoist southeast of Building 70022. facing northwest. Building ...
View of hoist southeast of Building 70022. facing northwest. Building 70022 is in background - Naval Ordnance Test Station Inyokern, Randsburg Wash Facility Target Test Towers, Tower Road, China Lake, Kern County, CA
Development of a lead-acid battery for a hybrid electric vehicle
NASA Astrophysics Data System (ADS)
Cooper, A.
In September 2000, a project reliable, highly optimized lead-acid battery (RHOLAB) started under the UK Foresight Vehicle Programme with the objective of developing an optimized lead-acid battery solution for hybrid electric vehicles. The work is based on a novel, individual, spirally-wound valve-regulated lead-acid 2 V cell optimized for HEV use and low variability. This cell is being used as a building block for the development of a complete battery pack that is managed at the cell level. Following bench testing, this battery pack is to be thoroughly evaluated by substituting it for the Ni-MH pack in a Honda Insight. The RHOLAB cell is based on the 8 Ah Hawker Cyclon cell which has been modified to have current take-off at both ends—the dual-tab design. In addition, a variant has been produced with modified cell chemistry to help deal with problems that can occur when these valve-regulated lead-acid battery (VRLA) cells operate in a partial-state-of-charge condition. The cells have been cycled to a specially formulated test cycle based on real vehicle data derived from testing the Honda Insight on the various test tracks at the Millbrook Proving Grounds in the UK. These cycling tests have shown that the lead-acid pack can be successfully cycled when subjected to the high current demands from the vehicle, which have been measured at up to 15 C on discharge and 8 C during regenerative recharging, and cycle life is looking very promising under this arduous test regime. Concurrent with this work, battery development has been taking place. It was decided early on to develop the 144 V battery as four 36 V modules. Data collection and control has been built-in and special steps taken to minimize the problems of interconnect in this complex system. Development of the battery modules is now at an advanced stage. The project plan then allows for extensive testing of the vehicle with its lead-acid battery at Millbrook so it can be compared with the benchmark tests which have already been carried out on the vehicle with its Ni-MH batteries.
Flammability tests for regulation of building and construction materials
K. Sumathipala
2006-01-01
The regulation of building materials and products for flammability is critical to ensure the safety of occupants in buildings and other structures. The involvement of exposed building materials and products in fires resulting in the loss of human life often spurs an increase in regulation and new test methods to address the problem. Flammability tests range from those...
2. EXTERIOR VIEW OF BUILDING 25B (TEST CHAMBER BUILDING) AND ...
2. EXTERIOR VIEW OF BUILDING 25B (TEST CHAMBER BUILDING) AND WIND TUNNEL, LOOKING NORTHWEST (1991). - Wright-Patterson Air Force Base, Area B, Buildings 25 & 24,10-foot & 20-foot Wind Tunnel Complex, Northeast side of block bounded by K, G, Third, & Fifth Streets, Dayton, Montgomery County, OH
NASA Astrophysics Data System (ADS)
Atkins, Chad G.; Buckley, Kevin; Chen, Deborah; Schulze, H. G.; Devine, Dana V.; Blades, Michael W.; Turner, Robin F. B.
2015-07-01
Modern transfusion medicine relies on the safe, secure, and cost-effective delivery of donated red blood cells (RBCs). Once isolated, RBCs are suspended in a defined additive solution and stored in plastic blood bags in which, over time, they undergo chemical, physiological, and morphological changes that may have a deleterious impact on some patients. Regulations limit the storage period to 42 days and the cells do not routinely undergo analytical testing before use. In this study, we use Raman spectroscopy to interrogate stored RBCs and we identify metabolic and cell-breakdown products, such as haemoglobin and membrane fragments, that build-up in the blood bags as the cells age. Our work points the way to the development of an instrument which could quickly and easily assess the biochemical nature of stored RBC units before they are transfused.
Cell-Free and In Vivo Characterization of Lux, Las, and Rpa Quorum Activation Systems in E. coli.
Halleran, Andrew D; Murray, Richard M
2018-02-16
Synthetic biologists have turned toward quorum systems as a path for building sophisticated microbial consortia that exhibit group decision making. Currently, however, even the most complex consortium circuits rely on only one or two quorum sensing systems, greatly restricting the available design space. High-throughput characterization of available quorum sensing systems is useful for finding compatible sets of systems that are suitable for a defined circuit architecture. Recently, cell-free systems have gained popularity as a test-bed for rapid prototyping of genetic circuitry. We take advantage of the transcription-translation cell-free system to characterize three commonly used Lux-type quorum activators, Lux, Las, and Rpa. We then compare the cell-free characterization to results obtained in vivo. We find significant genetic crosstalk in both the Las and Rpa systems and substantial signal crosstalk in Lux activation. We show that cell-free characterization predicts crosstalk observed in vivo.
50. EAST CORNER OF BUILDING 365 (ARMAMENT TESTING BUILDING) IN ...
50. EAST CORNER OF BUILDING 365 (ARMAMENT TESTING BUILDING) IN BASE SPARES AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME
49. NORTHEAST FRONT ELEVATION OF BUILDING 365 (ARMAMENT TESTING BUILDING) ...
49. NORTHEAST FRONT ELEVATION OF BUILDING 365 (ARMAMENT TESTING BUILDING) IN BASE SPARES AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME
Wang, Enyu; Kong, Xiangfei; Rong, Xian; Yao, Chengqiang; Yang, Hua; Qi, Chengying
2016-01-01
Phase change material (PCM) used in buildings can reduce the building energy consumption and indoor temperature fluctuation. A composite PCM has been fabricated by the binary eutectic mixture of tetradecanol (TD) and lauric acid (LA) absorbed into the expanded perlite (EP) using vacuum impregnation method, and its thermal conductivity was promoted by aluminium powder (AP) additive. Besides, the styrene-acrylic emulsion has been mixed with the composite PCM particles to form the protective film, so as to solve the problem of leakage. Thus, a novel PCM panel (PCMP) has been prepared using compression moulding forming method. The thermal property, microstructure characteristic, mechanical property, thermal conductivity, thermal reliability and leakage of the composite PCM have been investigated and analysed. Meanwhile, the thermal performance of the prepared PCMP was tested through PCMPs installed on the inside wall of a cell under outdoor climatic conditions. The composite PCM has a melting temperature of 24.9 °C, a freezing temperature of 25.2 °C, a melting latent heat of 78.2 J/g and a freezing latent heat of 81.3 J/g. The thermal conductivity test exposed that the thermal conductivity has been enhanced with the addition of AP and the latent heat has been decreased, but it still remains in a high level. The leakage test result has proven that liquid PCM leaking has been avoided by the surface film method. The thermal performance experiment has shown the significant function of PCMP about adjusting the indoor temperature and reducing the heats transferring between the wall inside and outside. In view of the thermal performance, mechanical property and thermal reliability results, it can be concluded that the prepared PCMP has a promising building application potential. PMID:28774020
Time-Lapse Video of SLS Engine Section Test Article Being Stacked at Michoud
2017-04-25
This time-lapse video shows the Space Launch System engine section structural qualification test article being stacked at NASA's Michoud Assembly Facility in New Orleans. The rocket's engine section is the bottom of the core stage and houses the four RS-25 engines. The engine section test article was moved to Michoud's Cell A in Building 110 for vertical stacking with hardware that simulates the rocket's liquid hydrogen tank, which is the fuel tank that joins to the engine section. Once stacked, the entire test article will load onto the barge Pegasus and ship to NASA's Marshall Space Flight Center in Huntsville, Alabama. There, it will be subjected to millions of pounds of force during testing to ensure the hardware can withstand the incredible stresses of launch.
SLS Engine Section Test Article Moved for Stacking at Michoud
2017-04-25
Stacking is underway for the Space Launch System core stage engine section structural qualification test article at NASA's Michoud Assembly Facility in New Orleans. The rocket's engine section is the bottom of the core stage and houses the four RS-25 engines. The engine section test article was moved to Michoud's Cell A in Building 110 for vertical stacking with hardware that simulates the rocket's liquid hydrogen tank, which is the fuel tank that joins to the engine section. Once stacked, the entire test article will load onto the barge Pegasus and ship to NASA's Marshall Space Flight Center in Huntsville, Alabama. There, it will be subjected to millions of pounds of force during testing to ensure the hardware can withstand the incredible stresses of launch.
Single-faced GRAYQB™: a radiation mapping device
DOE Office of Scientific and Technical Information (OSTI.GOV)
Mayer, J.; Farfan, E.; Immel, D.
2013-12-12
GrayQb{trademark} is a novel technology that has the potential to characterize radioactively contaminated areas such as hot cells, gloveboxes, small and large rooms, hallways, and waste tanks. The goal of GrayQb{trademark} is to speed the process of decontaminating these areas, which reduces worker exposures and promotes ALARA considerations. The device employs Phosphorous Storage Plate (PSP) technology as its primary detector material. PSPs, commonly used for medical applications and non-destructive testing, can be read using a commercially available scanner. The goal of GrayQb{trademark} technology is to locate, quantify, and identify the sources of contamination. The purpose of the work documented inmore » this report was to better characterize the performance of GrayQb{trademark} in its ability to present overlay images of the PSP image and the associated visual image of the location being surveyed. The results presented in this report are overlay images identifying the location of hot spots in both controlled and field environments. The GrayQb{trademark} technology has been mainly tested in a controlled environment with known distances and source characteristics such as specific known radionuclides, dose rates, and strength. The original concept for the GrayQb{trademark} device involved utilizing the six faces of a cube configuration and was designed to be positioned in the center of a contaminated area for 3D mapping. A smaller single-faced GrayQb{trademark}, dubbed GrayQb SF, was designed for the purpose of conducting the characterization testing documented in this report. This lighter 2D version is ideal for applications where entry ports are too small for a deployment of the original GrayQb™ version or where only a single surface is of interest. The shape, size, and weight of these two designs have been carefully modeled to account for most limitations encountered in hot cells, gloveboxes, and contaminated areas. GrayQb{trademark} and GrayQb{trademark} SF share the same fundamental detection system design (e.g., pinhole and PSPs). Therefore, performance tests completed on the single face GrayQB in this report is also applicable to the six- faced GrayQB (e.g., ambient light sensitivity and PSP response). This report details the characterization of the GrayQb{trademark} SF in both an uncontrolled environment; specifically, the Savannah River Site (SRS) Plutonium Fuel Form Facility in Building 235-F (Metallurgical Building) and controlled testing at SRS’s Health Physics Instrument Calibration Facility and SRS’s R&D Engineering Imaging and Radiation Systems Building. In this report, the resulting images from the Calibration Facility were obtained by overlaying the PSP and visual images manually using ImageJ. The resulting images from the Building 235-F tests presented in this report were produced using ImageJ and applying response trends developed from controlled testing results. The GrayQb{trademark} technology has been developed in two main stages at Savannah River National Laboratory (SRNL): 1) the GrayQb{trademark} development was supported by SRNL’s Laboratory Directed Research and Development Program and 2) the GrayQb{trademark} SF development and its testing in Building 235-F were supported by the Office of Deactivation and Decommissioning and Facility Engineering (EM-13), U.S. Department of Energy – Office of Environmental Management.« less
SPERTI, Instrument Cell Building (PER606). West facade. Camera facing northeast. ...
SPERT-I, Instrument Cell Building (PER-606). West facade. Camera facing northeast. Date: August 2003. INEEL negative no. HD-35-3-1 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID
SPERTI, Instrument Cell Building (PER606). East facade. Camera facing southwest. ...
SPERT-I, Instrument Cell Building (PER-606). East facade. Camera facing southwest. Date: August 2003. INEEL negative no. HD-35-3-2 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID
Final Environmental Assessment: Base-Wide Building Demolition Arnold Air Force Base, Tennessee
2006-02-01
Building • Engine Test Facility ( ETF )-B Exhauster • ETF -A Airside • ETF -A Exhauster • ETF -A Reefer • CE Facility • Rocket Storage • Von Karman Gas...Executive Order ESA Endangered Species Act ETF Engine Test Facility FamCamp Family Camping Area P:\\ARNOLDAFB\\333402DO42COMPLIANCE\\DEMOLITION...Fabrication Shop • Natural Resources Building • Salt Storage Building • Administration Building • Engine Test Facility ( ETF )-B Exhauster • ETF -A
NASA Technical Reports Server (NTRS)
Veres, Joseph P.
2003-01-01
The objective is to develop the capability to numerically model the performance of gas turbine engines used for aircraft propulsion. This capability will provide turbine engine designers with a means of accurately predicting the performance of new engines in a system environment prior to building and testing. The 'numerical test cell' developed under this project will reduce the number of component and engine tests required during development. As a result, the project will help to reduce the design cycle time and cost of gas turbine engines. This capability will be distributed to U.S. turbine engine manufacturers and air framers. This project focuses on goals of maintaining U.S. superiority in commercial gas turbine engine development for the aeronautics industry.
Rapid prototyping of microbial cell factories via genome-scale engineering.
Si, Tong; Xiao, Han; Zhao, Huimin
2015-11-15
Advances in reading, writing and editing genetic materials have greatly expanded our ability to reprogram biological systems at the resolution of a single nucleotide and on the scale of a whole genome. Such capacity has greatly accelerated the cycles of design, build and test to engineer microbes for efficient synthesis of fuels, chemicals and drugs. In this review, we summarize the emerging technologies that have been applied, or are potentially useful for genome-scale engineering in microbial systems. We will focus on the development of high-throughput methodologies, which may accelerate the prototyping of microbial cell factories. Copyright © 2014 Elsevier Inc. All rights reserved.
NREL/NASA Internal Short-Circuit Instigator in Lithium Ion Cells
DOE Office of Scientific and Technical Information (OSTI.GOV)
Keyser, Matthew; Long, Dirk; Pesaran, Ahmad
Lithium-ion cells provide the highest specific energy (>280 Wh/kg) and energy density (>600 Wh/L) rechargeable battery building block to date with the longest life. Electrode/electrolyte thermal instability and flammability of the electrolyte of Li-ion cells make them prone to catastrophic thermal runaway under some rare internal short circuit conditions. Despite extensive QC/QA, standardized industry safety testing, and over 18 years of manufacturing experience, major recalls have taken place and incidents still occur. Many safety incidents that take place in the field originate due to an internal short that was not detectable or predictable at the point of manufacture. The Internalmore » Short-Circuit Instigator can be used to study types of separators, non-flammable electrolytes, electrolyte additives, fusible tabs, propagation studies, and gas generation within a cell.« less
ETR COMPLEX. CAMERA FACING SOUTH. FROM BOTTOM OF VIEW TO ...
ETR COMPLEX. CAMERA FACING SOUTH. FROM BOTTOM OF VIEW TO TOP: MTR, MTR SERVICE BUILDING, ETR CRITICAL FACILITY, ETR CONTROL BUILDING (ATTACHED TO ETR), ETR BUILDING (HIGH-BAY), COMPRESSOR BUILDING (ATTACHED AT LEFT OF ETR), HEAT EXCHANGER BUILDING (JUST BEYOND COMPRESSOR BUILDING), COOLING TOWER PUMP HOUSE, COOLING TOWER. OTHER BUILDINGS ARE CONTRACTORS' CONSTRUCTION BUILDINGS. INL NEGATIVE NO. 56-4105. Unknown Photographer, ca. 1956 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
7. PHOTOCOPY, PLANS, ELEVATIONS, AND SECTION DRAWING FOR MISSILE TEST ...
7. PHOTOCOPY, PLANS, ELEVATIONS, AND SECTION DRAWING FOR MISSILE TEST AND ASSEMBLY BUILDING. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL
SPERTI contextual view of instrument cell building, PER606. South facade. ...
SPERT-I contextual view of instrument cell building, PER-606. South facade. Camera facing northwest. PBF Cooling Tower in view at right. High bay of PBF Reactor Building, PER-602, is further to right. PBF-625 at left edge of view. Date: August 2003. INEEL negative no. HD-35-3-4 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID
12. VIEW OF TYPICAL CELL LOCKING MECHANISM, BUILDING 220 CELL ...
12. VIEW OF TYPICAL CELL LOCKING MECHANISM, BUILDING 220 CELL BLOCK 'A'. THE FACE PLATE OF THE CELL LOCK IS SHOWN REMOVED, EXPOSING THE ELECTROMAGNETIC LOCKING MECHANISM COMPRISING OF 2 MICROSWITCHES FOR LOCK POSITION INDICATION (FRONT LEFT CENTER AND REAR RIGHT CENTER OF PANEL); KEY SLOT MECHANICAL LOCK; LOCK SPRING (UPPER RIGHT OF PANEL); ELECTRIC SOLENOID (BOTTOM RIGHT CORNER OF PANEL); AND MISCELLANEOUS MECHANICAL LINKAGES. - U.S. Naval Base, Pearl Harbor, Brig, Neville Way near Ninth Street at Marine Barracks, Pearl City, Honolulu County, HI
Analysis of variability in additive manufactured open cell porous structures.
Evans, Sam; Jones, Eric; Fox, Pete; Sutcliffe, Chris
2017-06-01
In this article, a novel method of analysing build consistency of additively manufactured open cell porous structures is presented. Conventionally, methods such as micro computed tomography or scanning electron microscopy imaging have been applied to the measurement of geometric properties of porous material; however, high costs and low speeds make them unsuitable for analysing high volumes of components. Recent advances in the image-based analysis of open cell structures have opened up the possibility of qualifying variation in manufacturing of porous material. Here, a photogrammetric method of measurement, employing image analysis to extract values for geometric properties, is used to investigate the variation between identically designed porous samples measuring changes in material thickness and pore size, both intra- and inter-build. Following the measurement of 125 samples, intra-build material thickness showed variation of ±12%, and pore size ±4% of the mean measured values across five builds. Inter-build material thickness and pore size showed mean ranges higher than those of intra-build, ±16% and ±6% of the mean material thickness and pore size, respectively. Acquired measurements created baseline variation values and demonstrated techniques suitable for tracking build deviation and inspecting additively manufactured porous structures to indicate unwanted process fluctuations.
Field Test Data for Detecting Vibrations of a Building Using High-Speed Video Cameras
2017-10-01
ARL-TR-8185 ● OCT 2017 US Army Research Laboratory Field Test Data for Detecting Vibrations of a Building Using High -Speed Video...Field Test Data for Detecting Vibrations of a Building Using High -Speed Video Cameras by Caitlin P Conn and Geoffrey H Goldman Sensors and...June 2016 – October 2017 4. TITLE AND SUBTITLE Field Test Data for Detecting Vibrations of a Building Using High -Speed Video Cameras 5a. CONTRACT
1. West elevations of barrier (Building 4216/E17) and Monitor Building ...
1. West elevations of barrier (Building 4216/E-17) and Monitor Building (4203/E-4). Barrier is built of wood infilled with earth, intended to protect Monitor Building from flying debris should anything at Test Stand 'A' explode. Building 4203/E-4 is built of reinforced concrete; equipment on top of it is cooling tower for refrigeration equipment in Test Stand 'A' machinery room. Electrical utility poles are typical at the facility, and carry 4,800 volts 3-phase alternating current. - Jet Propulsion Laboratory Edwards Facility, Test Stand A, Control Center, Edwards Air Force Base, Boron, Kern County, CA
Liu, Z; Stout, J E; Boldin, M; Rugh, J; Diven, W F; Yu, V L
1998-01-01
One copper-silver ionization system was sequentially installed onto the hot-water recirculation lines of two hospital buildings colonized with Legionella pneumophila, serogroup 1. A third building with the same water supply and also colonized with Legionella served as a control. Four weeks after activation of the system, distal site positivity for Legionella in the first test building dropped to zero. After operating for 16 weeks, the system was disconnected and installed onto the second test building. Twelve weeks of disinfection reduced the distal site positivity for Legionella in the second test building to zero. Legionella recolonization did not occur in the first test building for 6-12 weeks and in the second test building for 8-12 weeks after inactivation of the system. The control building remained Legionella-positive throughout the experimental period. A significantly higher copper concentration was found in the biofilm taken from a sampling device than in that from water. This is likely to be the reason that the copper-silver ionization system had the residual effect of preventing early recolonization. Our study raises the possibility that one copper-silver unit could be rotated among several buildings to maintain a Legionella-free environment. Such an approach may be cost-effective for buildings housing individuals at low risk for contracting legionnaires' disease.
2. ROCKET ENGINE TEST STAND, SHOWING TANK (BUILDING 1929) AND ...
2. ROCKET ENGINE TEST STAND, SHOWING TANK (BUILDING 1929) AND GARAGE (BUILDING 1930) AT LEFT REAR. Looking to west. - Edwards Air Force Base, X-15 Engine Test Complex, Rocket Engine & Complete X-15 Vehicle Test Stands, Rogers Dry Lake, east of runway between North Base & South Base, Boron, Kern County, CA
ETR BUILDING, TRA642. SOUTH SIDE VIEW INCLUDES SOUTH SIDES OF ...
ETR BUILDING, TRA-642. SOUTH SIDE VIEW INCLUDES SOUTH SIDES OF ETR BUILDING (HIGH ROOF LINE); ELECTRICAL BUILDING (ONE-STORY, MADE OF PUMICE BLOCKS), TRA-648; AND HEAT EXCHANGER BUILDING (WITH BUILDING NUMBERS), TRA-644. NOTE PROJECTION OF ELECTRICAL BUILDING AT LEFT EDGE OF VIEW. CAMERA FACES NORTH. INL NEGATIVE NO. HD46-37-3. Mike Crane, Photographer, 4/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
116. ARAI Details of hot cell section of building ARA626. ...
116. ARA-I Details of hot cell section of building ARA-626. Shows manipulator openings in operating face of hot cell, start/stop buttons, and other details. Norman Engineering Company 961/area/SF-626-E-6. Date: January 1959. Ineel index code no. 068-0626-10-613-102731. - Idaho National Engineering Laboratory, Army Reactors Experimental Area, Scoville, Butte County, ID
Robustness of neuroprosthetic decoding algorithms.
Serruya, Mijail; Hatsopoulos, Nicholas; Fellows, Matthew; Paninski, Liam; Donoghue, John
2003-03-01
We assessed the ability of two algorithms to predict hand kinematics from neural activity as a function of the amount of data used to determine the algorithm parameters. Using chronically implanted intracortical arrays, single- and multineuron discharge was recorded during trained step tracking and slow continuous tracking tasks in macaque monkeys. The effect of increasing the amount of data used to build a neural decoding model on the ability of that model to predict hand kinematics accurately was examined. We evaluated how well a maximum-likelihood model classified discrete reaching directions and how well a linear filter model reconstructed continuous hand positions over time within and across days. For each of these two models we asked two questions: (1) How does classification performance change as the amount of data the model is built upon increases? (2) How does varying the time interval between the data used to build the model and the data used to test the model affect reconstruction? Less than 1 min of data for the discrete task (8 to 13 neurons) and less than 3 min (8 to 18 neurons) for the continuous task were required to build optimal models. Optimal performance was defined by a cost function we derived that reflects both the ability of the model to predict kinematics accurately and the cost of taking more time to build such models. For both the maximum-likelihood classifier and the linear filter model, increasing the duration between the time of building and testing the model within a day did not cause any significant trend of degradation or improvement in performance. Linear filters built on one day and tested on neural data on a subsequent day generated error-measure distributions that were not significantly different from those generated when the linear filters were tested on neural data from the initial day (p<0.05, Kolmogorov-Smirnov test). These data show that only a small amount of data from a limited number of cortical neurons appears to be necessary to construct robust models to predict kinematic parameters for the subsequent hours. Motor-control signals derived from neurons in motor cortex can be reliably acquired for use in neural prosthetic devices. Adequate decoding models can be built rapidly from small numbers of cells and maintained with daily calibration sessions.
Energy Efficiency and Sustainability of Different Building Structures in Latvian Climate
NASA Astrophysics Data System (ADS)
Jakovičs, A.; Gendelis, S.; Bandeniece, L.
2015-11-01
Five experimental test buildings have been built in Riga, Latvia. They are identical except external walls for which different mainly regional building materials are used. Calculated U-values of the other walls, floor and ceiling are the same for each test building. Initial moisture influences the relative humidity of indoor air, which can be higher in the initial time period; as a result, heat transmittances are also very different and cause different heating/cooling energy consumption. Overheating risk in summer exists for test buildings with the smallest thermal inertia. Both summer and heating seasons have been analysed and differences between five test houses have been discussed in details.
Overview of ToxCast™ | Science Inventory | US EPA
In 2007, EPA launched ToxCast™ in order to develop a cost-effective approach for prioritizing the toxicity testing of large numbers of chemicals in a short period of time. Using data from state-of-the-art high throughput screening (HTS) bioassays developed in the pharmaceutical industry, ToxCast™ is building computational models to forecast the potential human toxicity of chemicals. These hazard predictions will provide EPA regulatory programs with science-based information helpful in prioritizing chemicals for more detailed toxicological evaluations, and lead to more efficient use of animal testing. In its first phase, ToxCast™ is profiling over 300 well-characterized chemicals (primarily pesticides) in over 400 HTS endpoints. These endpoints include biochemical assays of protein function, cell-based transcriptional reporter assays, multi-cell interaction assays, transcriptomics on primary cell cultures, and developmental assays in zebrafish embryos. Almost all of the compounds being examined in Phase 1 of ToxCast™ have been tested in traditional toxicology tests, including developmental toxicity, multi-generation studies, and sub-chronic and chronic rodent bioassays. ToxRefDB, a relational database being created to house this information, will contain nearly $1B worth of toxicity studies in animals when completed. ToxRefDB is integrated into a more comprehensive data management system developed by NCCT called ACToR (Aggregated Computational Toxicology
DOE Office of Scientific and Technical Information (OSTI.GOV)
Clevenger, E.
2018-02-02
This project looks at alternative water sources, specifically in the field of desalination and selective ion removal through capacitive deionization (CDI). It project aims to both scale up the desalination capabilities of CDI cells as well as determine the selectivity of CDI for particular ions. My task is to design and build cells that have reproducible performance and characterize the materials for building these cells. The scientific methods I’ve learned through my work in CDI and the data mining and analysis tools I’ve become familiar with through CAES will be important catalysts in my future success in a graduate program.more » The purpose of this presentation is to give a standard of practice for my current method of building of capacitive deionization cells. Parts 1 and 2 will be discussed in which the electrodes are prepared, and the cell is built.« less
View looking west at Test Stand 'A' complex in morning ...
View looking west at Test Stand 'A' complex in morning sun. View shows Monitor Building 4203/E-4 at left, barrier (Building 4216/E-17) to right of 4203/E-4, and Test Stand 'A' tower. Attached structure to lower left of tower is Test Stand 'A' machine room which contained refrigeration equipment. Building in right background with Test Stand 'A' tower shadow on it is Assembly Building 4288/E-89, built in 1984. Row of ground-mounted brackets in foreground was used to carry electrical cable and/or fuel lines. - Jet Propulsion Laboratory Edwards Facility, Test Stand A, Edwards Air Force Base, Boron, Kern County, CA
Genotoxic potential evaluation of a cosmetic insoluble substance by the micronuclei assay.
Dayan, N; Shah, V; Minko, T
2011-01-01
An optical brightener (OB) powder (INCI: sodium silicoaluminate (and) glycidoxypropyl trimethyloxysilane/PEI-250 cross fluorescent brightener 230 salt (and) polyvinylalcohol crosspolymer) that is used in cosmetic facial products was tested for its genotoxic potential using the micronuclei test (MNT). It is a solid dry powder with an average size of 5 microns that is insoluble but dispersible in water. This study describes the exposure of cell culture to positive controls with and without enzymatic activation and to the test compound in different concentrations. We evaluated three end points: microscopic observation and quantification of micronuclei formation, and cell viability and proliferation. Both positive controls induced significant changes that were observed under the microscope and quantified. Based on its chemical nature, it was not anticipated that the test substance will degrade under the conditions of the experiments. However, the test is required to make sure that when solublized, impurities that may be present, even at trace levels, will not induce a genotoxic effect. The test compound did not promote micronuclei formation or change the viability or proliferation rate of cells. During this study we faced challenges such as solubilization and correlating viability data to genotoxicity data. These are described in the body of the paper. We believe that with the emergence of the 7(th) European amendment that bans animal testing, sharing these data and the study protocol serves as a key in building the understanding of the utilization of in vitro studies in the safety assessment of cosmetic ingredients.
ETRMTR MECHANICAL SERVICES BUILDING, TRA653. CAMERA FACING NORTHWEST AS BUILDING ...
ETR-MTR MECHANICAL SERVICES BUILDING, TRA-653. CAMERA FACING NORTHWEST AS BUILDING WAS NEARLY COMPLETE. INL NEGATIVE NO. 57-3653. K. Mansfield, Photographer, 7/22/1957 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
DOE Office of Scientific and Technical Information (OSTI.GOV)
Judkoff, R.; Neymark, J.
2013-07-01
ANSI/ASHRAE Standard 140, Standard Method of Test for the Evaluation of Building Energy Analysis Computer Programs applies the IEA BESTEST building thermal fabric test cases and example simulation results originally published in 1995. These software accuracy test cases and their example simulation results, which comprise the first test suite adapted for the initial 2001 version of Standard 140, are approaching their 20th anniversary. In response to the evolution of the state of the art in building thermal fabric modeling since the test cases and example simulation results were developed, work is commencing to update the normative test specification and themore » informative example results.« less
DOE Office of Scientific and Technical Information (OSTI.GOV)
Judkoff, R.; Neymark, J.
2013-07-01
ANSI/ASHRAE Standard 140, Standard Method of Test for the Evaluation of Building Energy Analysis Computer Programs applies the IEA BESTEST building thermal fabric test cases and example simulation results originally published in 1995. These software accuracy test cases and their example simulation results, which comprise the first test suite adapted for the initial 2001 version of Standard 140, are approaching their 20th anniversary. In response to the evolution of the state of the art in building thermal fabric modeling since the test cases and example simulation results were developed, work is commencing to update the normative test specification and themore » informative example results.« less
The Effect of Body Build and BMI on Aerobic Test Performance in School Children (10-15 Years)
Slinger, Jantine; Verstappen, Frans; Breda, Eric Van; Kuipers, Harm
2006-01-01
Body Mass Index (BMI) has often questionably been used to define body build. In the present study body build was defined more specifically using fat free mass index (FFMI = fat free mass normalised to the stature) and fat mass index (FMI = fat mass normalised to stature). The body build of an individual is ‘solid’ in individuals with a high FFMI for their FMI and is ‘slender’ in individuals with a low FFMI relative to their FMI. The aim of the present study was to investigate the association between aerobic test performance and body build defined as solid, average or slender in 10 to 15 year old children. Five-hundred-and-two children (53% boys) aged 10 to 15 years of age were included in the study. Aerobic test performance was estimated with an incremental cycle ergometer protocol and a shuttle run test. BMI and percentage fat (by skin folds) were determined to calculate FMI and FFMI. After adjustment for differences in age, gender and body mass the solid group achieved a significantly higher maximal power output (W) and power output relative to body mass (W/kg) during the cycle test (p < 0.05) and a higher shuttle-run score (p < 0.05) compared to the slender group. The power output relative to FFM (W/kg FFM) was comparable (p > 0.05) between different body build groups. This study showed that body build is an important determinant of the aerobic test performance. In contrast, there were no differences in aerobic test performance per kilogramme FFM over the body build groups. This suggests that the body build may be determined by genetic predisposition. Key Points Children with a solid body build perform better in aerobic exercise tests than slender children. The power output relative to fat free mass was comparable in the solid, slender and average group. Besides body composition, body build should be considered related to other performance measurements. PMID:24357967
1. EXTERIOR VIEW, LOOKING SOUTHEAST, SHOWING FLIGHT TEST HANGARS IN ...
1. EXTERIOR VIEW, LOOKING SOUTHEAST, SHOWING FLIGHT TEST HANGARS IN CENTER, BUILDING 7 ON LEFT, AND BUILDING 8 ON RIGHT. - Wright-Patterson Air Force Base, Area B, Building 1/9, Flight Test Hangars, On flightline between Ninth & Tenth Streets, Dayton, Montgomery County, OH
NASA Astrophysics Data System (ADS)
Kobayashi, K.; Isobe, K.; Iwai, Y.; Hayashi, T.; Shu, W.; Nakamura, H.; Kawamura, Y.; Yamada, M.; Suzuki, T.; Miura, H.; Uzawa, M.; Nishikawa, M.; Yamanishi, T.
2007-12-01
Confinement and the removal of tritium are key subjects for the safety of ITER. The ITER buildings are confinement barriers of tritium. In a hot cell, tritium is often released as vapour and is in contact with the inner walls. The inner walls of the ITER tritium plant building will also be exposed to tritium in an accident. The tritium released in the buildings is removed by the atmosphere detritiation systems (ADS), where the tritium is oxidized by catalysts and is removed as water. A special gas of SF6 is used in ITER and is expected to be released in an accident such as a fire. Although the SF6 gas has potential as a catalyst poison, the performance of ADS with the existence of SF6 has not been confirmed as yet. Tritiated water is produced in the regeneration process of ADS and is subsequently processed by the ITER water detritiation system (WDS). One of the key components of the WDS is an electrolysis cell. To overcome the issues in a global tritium confinement, a series of experimental studies have been carried out as an ITER R&D task: (1) tritium behaviour in concrete; (2) the effect of SF6 on the performance of ADS and (3) tritium durability of the electrolysis cell of the ITER-WDS. (1) The tritiated water vapour penetrated up to 50 mm into the concrete from the surface in six months' exposure. The penetration rate of tritium in the concrete was thus appreciably first, the isotope exchange capacity of the cement paste plays an important role in tritium trapping and penetration into concrete materials when concrete is exposed to tritiated water vapour. It is required to evaluate the effect of coating on the penetration rate quantitatively from the actual tritium tests. (2) SF6 gas decreased the detritiation factor of ADS. Since the effect of SF6 depends closely on its concentration, the amount of SF6 released into the tritium handling area in an accident should be reduced by some ideas of arrangement of components in the buildings. (3) It was expected that the electrolysis cell of the ITER-WDS could endure 3 years' operation under the ITER design conditions. Measuring the concentration of the fluorine ions could be a promising technique for monitoring the damage to the electrolysis cell.
35. Historic photo of Building 202 test stand with damage ...
35. Historic photo of Building 202 test stand with damage to twenty-thousand-pound-thrust rocket engine related to failure during testing, September 16, 1958. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-48704. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
SPERTI, Instrument Cell Building (PER606). Oblique view of north and ...
SPERT-I, Instrument Cell Building (PER-606). Oblique view of north and east facades. Camera facing southwest. Date: August 2003. INEEL negative no. HD-35-4-1 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID
VIEW OF FECF HOT CELL OF FUEL STORAGE BUILDING (CPP603). ...
VIEW OF FECF HOT CELL OF FUEL STORAGE BUILDING (CPP-603). PHOTO TAKEN LOOKING NORHTWEST. INL PHOTO NUMBER HD-54-18-3. Mike Crane, Photographer, 8/2005 - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID
PLAN SECTIONS AND DETAILS OF CELL HATCHES MAIN PROCESSING BUILDING ...
PLAN SECTIONS AND DETAILS OF CELL HATCHES MAIN PROCESSING BUILDING (CPP-601). INL DRAWING NUMBER 200-0601-00-291-103256. ALTERNATE ID NUMBER 542-11-F-302. - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID
Modeling of cytometry data in logarithmic space: When is a bimodal distribution not bimodal?
Erez, Amir; Vogel, Robert; Mugler, Andrew; Belmonte, Andrew; Altan-Bonnet, Grégoire
2018-02-16
Recent efforts in systems immunology lead researchers to build quantitative models of cell activation and differentiation. One goal is to account for the distributions of proteins from single-cell measurements by flow cytometry or mass cytometry as readout of biological regulation. In that context, large cell-to-cell variability is often observed in biological quantities. We show here that these readouts, viewed in logarithmic scale may result in two easily-distinguishable modes, while the underlying distribution (in linear scale) is unimodal. We introduce a simple mathematical test to highlight this mismatch. We then dissect the flow of influence of cell-to-cell variability proposing a graphical model which motivates higher-dimensional analysis of the data. Finally we show how acquiring additional biological information can be used to reduce uncertainty introduced by cell-to-cell variability, helping to clarify whether the data is uni- or bimodal. This communication has cautionary implications for manual and automatic gating strategies, as well as clustering and modeling of single-cell measurements. © 2018 International Society for Advancement of Cytometry. © 2018 International Society for Advancement of Cytometry.
STS-51 preparation: ACTS, ORFEUS, Discovery in VAB
NASA Technical Reports Server (NTRS)
1993-01-01
In NASA's building AM on Cape Canaveral Air Force Station, STS-51 mission specialist Carl Walz (right) and Deutsche Aerospace technician Gregor Dawidowitsch check over the scientific instruments mounted on the Shuttle Pallet Satellite (SPAS) carrier (38573); The Orbiting and Retrievable Far and Extreme Ultraviolet Spectrometer (ORFEUS) and SPAS is readied for hoisting into a test cell at the Vertical Processing Facility (VPF) (38574); Mating of the Advanced Communications Technology Satellite (ACTS) with the Transfer Orbit Stage (TOS) booster is under way in the Payload Hazardous Servicing Facility (PHSF) (38575); The mated ACTS and TOS are ready to be moved from the PHSF to the Vertical Processsing Facility (VPF) (38576); The orbiter Discovery is rolled into the Vehicle Assembly Building (VAB) for mating with the external tank and twin solid rocket boosters (38577-8).
Yadav, Vinod Kumar; Kumar, Akinchan; Mann, Anita; Aggarwal, Suruchi; Kumar, Maneesh; Roy, Sumitabho Deb; Pore, Subrata Kumar; Banerjee, Rajkumar; Mahesh Kumar, Jerald; Thakur, Ram Krishna; Chowdhury, Shantanu
2014-01-01
Building molecular correlates of drug resistance in cancer and exploiting them for therapeutic intervention remains a pressing clinical need. To identify factors that impact drug resistance herein we built a model that couples inherent cell-based response toward drugs with transcriptomes of resistant/sensitive cells. To test this model, we focused on a group of genes called metastasis suppressor genes (MSGs) that influence aggressiveness and metastatic potential of cancers. Interestingly, modeling of 84 000 drug response transcriptome combinations predicted multiple MSGs to be associated with resistance of different cell types and drugs. As a case study, on inducing MSG levels in a drug resistant breast cancer line resistance to anticancer drugs caerulomycin, camptothecin and topotecan decreased by more than 50-60%, in both culture conditions and also in tumors generated in mice, in contrast to control un-induced cells. To our knowledge, this is the first demonstration of engineered reversal of drug resistance in cancer cells based on a model that exploits inherent cellular response profiles.
1. BUILDING 8814, NORTH FRONT AND WEST SIDE. BUILDING 8832, ...
1. BUILDING 8814, NORTH FRONT AND WEST SIDE. BUILDING 8832, TEST STAND 1-E, IN LEFT DISTANCE. Looking southeast. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Observation Bunker 1-D-3, Test Area 1-125, northwest end of Altair Boulevard, Boron, Kern County, CA
ETR HEAT EXCHANGER BUILDING, TRA644. DETAIL OF SOUTH SIDE BUILDING ...
ETR HEAT EXCHANGER BUILDING, TRA-644. DETAIL OF SOUTH SIDE BUILDING INSET. DEMINERALIZER WING AT RIGHT. CAMERA FACING NORTH. INL NEGATIVE NO. HD46-36-2. Mike Crane, Photographer, 4/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
31. Historic view of Building 202 test stand A with ...
31. Historic view of Building 202 test stand A with rocket engine, November 19, 1957. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA GRC photo number C-46491. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
2013-07-26
CAPE CANAVERAL, Fla. – The Orion crew module for Exploration Flight Test 1 sits inside a clean room processing cell in the Operations and Checkout Building high bay at NASA’s Kennedy Space Center in Florida. Orion is the exploration spacecraft designed to carry crews to space beyond low Earth orbit. It will provide emergency abort capability, sustain the crew during the space travel and provide safe re-entry from deep space return velocities. The first unpiloted test flight of the Orion is scheduled to launch in 2014 atop a Delta IV rocket and in 2017 on a Space Launch System rocket. For more information, visit http://www.nasa.gov/orion. Photo credit: NASA/Jim Grossmann
2013-07-26
CAPE CANAVERAL, Fla. – The Orion crew module for Exploration Flight Test 1 sits inside a clean room processing cell in the Operations and Checkout Building high bay at NASA’s Kennedy Space Center in Florida. Orion is the exploration spacecraft designed to carry crews to space beyond low Earth orbit. It will provide emergency abort capability, sustain the crew during the space travel and provide safe re-entry from deep space return velocities. The first unpiloted test flight of the Orion is scheduled to launch in 2014 atop a Delta IV rocket and in 2017 on a Space Launch System rocket. For more information, visit http://www.nasa.gov/orion. Photo credit: NASA/Jim Grossmann
2010-09-29
CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, workers check the hoist connections on External Fuel Tank-122 as it is lifted toward a test cell. ET-122, the Space Shuttle Program's last external fuel tank was delivered to Kennedy's Turn Basin from NASA’s Michoud Assembly Facility in New Orleans aboard the Pegasus Barge. After testing, ET-122 eventually will be attached to space shuttle Endeavour for the STS-134 mission to the International Space Station targeted to launch February, 2011. For more information visit: http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Dimitri Gerondidakis
2010-09-29
CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, workers secure wires from an overhead hoist to External Fuel Tank-122, for its lift into a test cell. ET-122, the Space Shuttle Program's last external fuel tank was delivered to Kennedy's Turn Basin from NASA’s Michoud Assembly Facility in New Orleans aboard the Pegasus Barge. After testing, ET-122 eventually will be attached to space shuttle Endeavour for the STS-134 mission to the International Space Station targeted to launch February, 2011. For more information visit: http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Dimitri Gerondidakis
2010-09-29
CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, workers monitor the progress of External Fuel Tank-122 as it is lifted toward a test cell. ET-122, the Space Shuttle Program's last external fuel tank was delivered to Kennedy's Turn Basin from NASA’s Michoud Assembly Facility in New Orleans aboard the Pegasus Barge. After testing, ET-122 eventually will be attached to space shuttle Endeavour for the STS-134 mission to the International Space Station targeted to launch February, 2011. For more information visit: http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Dimitri Gerondidakis
2010-09-29
CAPE CANAVERAL, Fla. -- At NASA’s Kennedy Space Center in Florida, External Fuel Tank-122 is lifted high over the transfer aisle of the Vehicle Assembly Building during operations to transfer it into a test cell. ET-122, the Space Shuttle Program's last external fuel tank was delivered to Kennedy's Turn Basin from NASA’s Michoud Assembly Facility in New Orleans aboard the Pegasus Barge. After testing, ET-122 eventually will be attached to space shuttle Endeavour for the STS-134 mission to the International Space Station targeted to launch February, 2011. For more information visit: http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Dimitri Gerondidakis
2010-09-29
CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, External Fuel Tank-122 sits on its transporter in the transfer aisle waiting to be lifted into a test cell. ET-122, the Space Shuttle Program's last external fuel tank was delivered to Kennedy's Turn Basin from NASA’s Michoud Assembly Facility in New Orleans aboard the Pegasus Barge. After testing, ET-122 eventually will be attached to space shuttle Endeavour for the STS-134 mission to the International Space Station targeted to launch February, 2011. For more information visit: http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Dimitri Gerondidakis
2010-09-29
CAPE CANAVERAL, Fla. -- At NASA’s Kennedy Space Center in Florida, External Fuel Tank-122 is suspended vertically over the transfer aisle of the Vehicle Assembly Building as it is lifted toward a test cell. ET-122, the Space Shuttle Program's last external fuel tank was delivered to Kennedy's Turn Basin from NASA’s Michoud Assembly Facility in New Orleans aboard the Pegasus Barge. After testing, ET-122 eventually will be attached to space shuttle Endeavour for the STS-134 mission to the International Space Station targeted to launch February, 2011. For more information visit: http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Dimitri Gerondidakis
2010-09-29
CAPE CANAVERAL, Fla. -- In the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, workers check the hoist connections on External Fuel Tank-122 as it is lifted toward a test cell. ET-122, the Space Shuttle Program's last external fuel tank was delivered to Kennedy's Turn Basin from NASA’s Michoud Assembly Facility in New Orleans aboard the Pegasus Barge. After testing, ET-122 eventually will be attached to space shuttle Endeavour for the STS-134 mission to the International Space Station targeted to launch February, 2011. For more information visit: http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Dimitri Gerondidakis
Saikaly, Pascal E.; Barlaz, Morton A.; de los Reyes, Francis L.
2007-01-01
Evaluation of the fate and transport of biological warfare (BW) agents in landfills requires the development of specific and sensitive detection assays. The objective of the current study was to develop and validate SYBR green quantitative real-time PCR (Q-PCR) assays for the specific detection and quantification of surrogate BW agents in synthetic building debris (SBD) and leachate. Bacillus atrophaeus (vegetative cells and spores) and Serratia marcescens were used as surrogates for Bacillus anthracis (anthrax) and Yersinia pestis (plague), respectively. The targets for SYBR green Q-PCR assays were the 16S-23S rRNA intergenic transcribed spacer (ITS) region and recA gene for B. atrophaeus and the gyrB, wzm, and recA genes for S. marcescens. All assays showed high specificity when tested against 5 ng of closely related Bacillus and Serratia nontarget DNA from 21 organisms. Several spore lysis methods that include a combination of one or more of freeze-thaw cycles, chemical lysis, hot detergent treatment, bead beat homogenization, and sonication were evaluated. All methods tested showed similar threshold cycle values. The limit of detection of the developed Q-PCR assays was determined using DNA extracted from a pure bacterial culture and DNA extracted from sterile water, leachate, and SBD samples spiked with increasing quantities of surrogates. The limit of detection for B. atrophaeus genomic DNA using the ITS and B. atrophaeus recA Q-PCR assays was 7.5 fg per PCR. The limits of detection of S. marcescens genomic DNA using the gyrB, wzm, and S. marcescens recA Q-PCR assays were 7.5 fg, 75 fg, and 7.5 fg per PCR, respectively. Quantification of B. atrophaeus vegetative cells and spores was linear (R2 > 0.98) over a 7-log-unit dynamic range down to 101 B. atrophaeus cells or spores. Quantification of S. marcescens (R2 > 0.98) was linear over a 6-log-unit dynamic range down to 102 S. marcescens cells. The developed Q-PCR assays are highly specific and sensitive and can be used for monitoring the fate and transport of the BW surrogates B. atrophaeus and S. marcescens in building debris and leachate. PMID:17720820
Investigation of Concrete Floor Vibration Using Heel-Drop Test
NASA Astrophysics Data System (ADS)
Azaman, N. A. Mohd; Ghafar, N. H. Abd; Azhar, A. F.; Fauzi, A. A.; Ismail, H. A.; Syed Idrus, S. S.; Mokhjar, S. S.; Hamid, F. F. Abd
2018-04-01
In recent years, there is an increased in floor vibration problems of structures like residential and commercial building. Vibration is defined as a serviceability issue related to the comfort of the occupant or damage equipment. Human activities are the main source of vibration in the building and it could affect the human comfort and annoyance of residents in the building when the vibration exceed the recommend level. A new building, Madrasah Tahfiz located at Yong Peng have vibration problem when load subjected on the first floor of the building. However, the limitation of vibration occurs on building is unknown. Therefore, testing is needed to determine the vibration behaviour (frequency, damping ratio and mode shape) of the building. Heel-drop with pace 2Hz was used in field measurement to obtain the vibration response. Since, the heel-drop test results would vary in light of person performance, test are carried out three time to reduce uncertainty. Natural frequency from Frequency Response Function analysis (FRF) is 17.4Hz, 16.8, 17.4Hz respectively for each test.
ETR HEAT EXCHANGER BUILDING, TRA644. METAL FRAME OF BUILDING GOES ...
ETR HEAT EXCHANGER BUILDING, TRA-644. METAL FRAME OF BUILDING GOES UP IN BACKGROUND AS WORKERS PLACE A SECTION OF WATER LINE THAT WILL CARRY SECONDARY COOLANT BETWEEN HEAT EXCHANGER BUILDING AND THE COOLING TOWER. INL NEGATIVE NO. 56-2205. Jack L. Anderson, Photographer, 6/28/1956 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
2014-06-08
CAPE CANAVERAL, Fla. -- Inside the Operations and Checkout Building high bay at NASA's Kennedy Space Center in Florida, the Orion service module has been secured in the Final Assembly and System Testing, or FAST, cell. The Orion crew module will be stacked on the service module in the FAST cell and then both modules will be put through their final system tests for Exploration Flight Test-1, or EFT-1, before rolling out of the facility for integration with the United Launch Alliance Delta IV Heavy rocket. Orion is the exploration spacecraft designed to carry astronauts to destinations not yet explored by humans, including an asteroid and Mars. It will have emergency abort capability, sustain the crew during space travel and provide safe re-entry from deep space return velocities. The first unpiloted test flight of Orion, EFT-1, is scheduled to launch later this year atop a Delta IV rocket from Cape Canaveral Air Force Station in Florida to an altitude of 3,600 miles above the Earth's surface. The two-orbit, four-hour flight test will help engineers evaluate the systems critical to crew safety including the heat shield, parachute system and launch abort system. For more information, visit http://www.nasa.gov/orion. Photo credit: NASA/Glenn Benson
2014-06-08
CAPE CANAVERAL, Fla. -- Inside the Operations and Checkout Building high bay at NASA's Kennedy Space Center in Florida, the Orion service module has been secured in the Final Assembly and System Testing, or FAST, cell. The Orion crew module will be stacked on the service module in the FAST cell and then both modules will be put through their final system tests for Exploration Flight Test-1, or EFT-1, before rolling out of the facility for integration with the United Launch Alliance Delta IV Heavy rocket. Orion is the exploration spacecraft designed to carry astronauts to destinations not yet explored by humans, including an asteroid and Mars. It will have emergency abort capability, sustain the crew during space travel and provide safe re-entry from deep space return velocities. The first unpiloted test flight of Orion, EFT-1, is scheduled to launch later this year atop a Delta IV rocket from Cape Canaveral Air Force Station in Florida to an altitude of 3,600 miles above the Earth's surface. The two-orbit, four-hour flight test will help engineers evaluate the systems critical to crew safety including the heat shield, parachute system and launch abort system. For more information, visit http://www.nasa.gov/orion. Photo credit: NASA/Glenn Benson
Computer-aided design of biological circuits using TinkerCell
Bergmann, Frank T; Sauro, Herbert M
2010-01-01
Synthetic biology is an engineering discipline that builds on modeling practices from systems biology and wet-lab techniques from genetic engineering. As synthetic biology advances, efficient procedures will be developed that will allow a synthetic biologist to design, analyze and build biological networks. In this idealized pipeline, computer-aided design (CAD) is a necessary component. The role of a CAD application would be to allow efficient transition from a general design to a final product. TinkerCell is a design tool for serving this purpose in synthetic biology. In TinkerCell, users build biological networks using biological parts and modules. The network can be analyzed using one of several functions provided by TinkerCell or custom programs from third-party sources. Since best practices for modeling and constructing synthetic biology networks have not yet been established, TinkerCell is designed as a flexible and extensible application that can adjust itself to changes in the field. PMID:21327060
Svingen, Terje; Koopman, Peter
2013-01-01
Development of testes in the mammalian embryo requires the formation and assembly of several cell types that allow these organs to achieve their roles in male reproduction and endocrine regulation. Testis development is unusual in that several cell types such as Sertoli, Leydig, and spermatogonial cells arise from bipotential precursors present in the precursor tissue, the genital ridge. These cell types do not differentiate independently but depend on signals from Sertoli cells that differentiate under the influence of transcription factors SRY and SOX9. While these steps are becoming better understood, the origins and roles of many testicular cell types and structures—including peritubular myoid cells, the tunica albuginea, the arterial and venous blood vasculature, lymphatic vessels, macrophages, and nerve cells—have remained unclear. This review synthesizes current knowledge of how the architecture of the testis unfolds and highlights the questions that remain to be explored, thus providing a roadmap for future studies that may help illuminate the causes of XY disorders of sex development, infertility, and testicular cancers. PMID:24240231
2. TEST AREA 1115, A VIEW TO THE SOUTHEAST FROM ...
2. TEST AREA 1-115, A VIEW TO THE SOUTHEAST FROM THE DECK OF TEST STAND 1-5. AT RIGHT IS BUILDING 8642, MACHINE SHOP FOR TEST STAND 1-5. AT LEFT IS BUILDING 8649, AND PART OF BUILDING 8647, TEST STAND 1-4, IS VISIBLE TO LEFT OF BLDG. 8649. (PANORAMA 1/2). - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Leuhman Ridge near Highways 58 & 395, Boron, Kern County, CA
EQUIPMENT LAYOUT OF MAIN PROCESSING BUILDING (CPP601) LCELL PLAN AND ...
EQUIPMENT LAYOUT OF MAIN PROCESSING BUILDING (CPP-601) L-CELL PLAN AND SECTION SHOWS COMPLEXITY OF CELLS. INL DRAWING NUMBER 200-0601-00-098-105687. ALTERNATE ID NUMBER 4289-20-301. - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID
Webinar August 11: Analysis Using Fuel Cell MHE for Shaving Peak Building
;Analysis Using Fuel Cell Material Handling Equipment (MHE) for Shaving Peak Building Energy" on offset grid charges associated with peak facility demands. The analyzed scenarios will focus on how the alternative peak-shaving apparatus. View the past webinar. -Sara Havig
113. ARAI Hot cell (ARA626) Building wall sections and details ...
113. ARA-I Hot cell (ARA-626) Building wall sections and details of radio chemistry lab. Shows high-bay roof over hot cells and isolation rooms below grade storage pit for fuel elements. Norman Engineering Company: 961-area/SF-626-A-4. Date: January 1959. Ineel index code no. 068-0626-00-613-102724. - Idaho National Engineering Laboratory, Army Reactors Experimental Area, Scoville, Butte County, ID
SPERTI Reactor Pit Building (PER605). Earth shielding protect adjacent Instrument ...
SPERT-I Reactor Pit Building (PER-605). Earth shielding protect adjacent Instrument Cell (PER-606). Security fencing surrounds complex, to which gate entry is provided next to Guard House (PER-607). Note gravel road leading to control area. Earth-covered conduit leads from instrument cell to terminal building out of view. Photographer: R.G. Larsen. Date: June 22, 1955. INEEL negative no. 55-1701 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID
1. GENERAL VIEW OF OBSERVATION BUNKERS TO THE EAST OF ...
1. GENERAL VIEW OF OBSERVATION BUNKERS TO THE EAST OF TEST STAND 1-A: IN FOREGROUND, BUILDING 8769, A TWO WINDOW DESIGN (NOT ON SITE PLAN); IN THE MIDDLE DISTANCE, BUILDING 8768, A ONE WINDOW DESIGN "OBSERVATION AND CAMERA BUILDING NO. 2" ON SITE PLAN; IN THE DISTANCE, AT FAR LEFT, BUILDING 8767, A FOUR WINDOW DESIGN "MASTER OBSERVATION AND CAMERA BUILDING NO. 1" ON SITE PLAN. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Observation Bunkers for Test Stand 1-A, Test Area 1-120, north end of Jupiter Boulevard, Boron, Kern County, CA
COMPRESSOR BUILDING, TRA626. ELEVATIONS. WINDOWS. WALL SECTIONS. PUMICE BLOCK BUILDING ...
COMPRESSOR BUILDING, TRA-626. ELEVATIONS. WINDOWS. WALL SECTIONS. PUMICE BLOCK BUILDING HOUSED COMPRESSORS FOR AIRCRAFT NUCLEAR PROPULSION EXPERIMENTS. MTR-626-IDO-2S, 3/1952. INL INDEX NO. 531-0626-00-396-110535, REV. 2. - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
Engineering the extracellular environment: Strategies for building 2D and 3D cellular structures.
Guillame-Gentil, Orane; Semenov, Oleg; Roca, Ana Sala; Groth, Thomas; Zahn, Raphael; Vörös, Janos; Zenobi-Wong, Marcy
2010-12-21
Cell fate is regulated by extracellular environmental signals. Receptor specific interaction of the cell with proteins, glycans, soluble factors as well as neighboring cells can steer cells towards proliferation, differentiation, apoptosis or migration. In this review, approaches to build cellular structures by engineering aspects of the extracellular environment are described. These methods include non-specific modifications to control the wettability and stiffness of surfaces using self-assembled monolayers (SAMs) and polyelectrolyte multilayers (PEMs) as well as methods where the temporal activation and spatial distribution of adhesion ligands is controlled. Building on these techniques, construction of two-dimensional cell sheets using temperature sensitive polymers or electrochemical dissolution is described together with current applications of these grafts in the clinical arena. Finally, methods to pattern cells in three-dimensions as well as to functionalize the 3D environment with biologic motifs take us one step closer to being able to engineer multicellular tissues and organs. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Kaspi, Omer; Yosipof, Abraham; Senderowitz, Hanoch
2017-06-06
An important aspect of chemoinformatics and material-informatics is the usage of machine learning algorithms to build Quantitative Structure Activity Relationship (QSAR) models. The RANdom SAmple Consensus (RANSAC) algorithm is a predictive modeling tool widely used in the image processing field for cleaning datasets from noise. RANSAC could be used as a "one stop shop" algorithm for developing and validating QSAR models, performing outlier removal, descriptors selection, model development and predictions for test set samples using applicability domain. For "future" predictions (i.e., for samples not included in the original test set) RANSAC provides a statistical estimate for the probability of obtaining reliable predictions, i.e., predictions within a pre-defined number of standard deviations from the true values. In this work we describe the first application of RNASAC in material informatics, focusing on the analysis of solar cells. We demonstrate that for three datasets representing different metal oxide (MO) based solar cell libraries RANSAC-derived models select descriptors previously shown to correlate with key photovoltaic properties and lead to good predictive statistics for these properties. These models were subsequently used to predict the properties of virtual solar cells libraries highlighting interesting dependencies of PV properties on MO compositions.
A Fully Automated High-Throughput Zebrafish Behavioral Ototoxicity Assay.
Todd, Douglas W; Philip, Rohit C; Niihori, Maki; Ringle, Ryan A; Coyle, Kelsey R; Zehri, Sobia F; Zabala, Leanne; Mudery, Jordan A; Francis, Ross H; Rodriguez, Jeffrey J; Jacob, Abraham
2017-08-01
Zebrafish animal models lend themselves to behavioral assays that can facilitate rapid screening of ototoxic, otoprotective, and otoregenerative drugs. Structurally similar to human inner ear hair cells, the mechanosensory hair cells on their lateral line allow the zebrafish to sense water flow and orient head-to-current in a behavior called rheotaxis. This rheotaxis behavior deteriorates in a dose-dependent manner with increased exposure to the ototoxin cisplatin, thereby establishing itself as an excellent biomarker for anatomic damage to lateral line hair cells. Building on work by our group and others, we have built a new, fully automated high-throughput behavioral assay system that uses automated image analysis techniques to quantify rheotaxis behavior. This novel system consists of a custom-designed swimming apparatus and imaging system consisting of network-controlled Raspberry Pi microcomputers capturing infrared video. Automated analysis techniques detect individual zebrafish, compute their orientation, and quantify the rheotaxis behavior of a zebrafish test population, producing a powerful, high-throughput behavioral assay. Using our fully automated biological assay to test a standardized ototoxic dose of cisplatin against varying doses of compounds that protect or regenerate hair cells may facilitate rapid translation of candidate drugs into preclinical mammalian models of hearing loss.
NREL Improves Building Energy Simulation Programs Through Diagnostic Testing (Fact Sheet)
DOE Office of Scientific and Technical Information (OSTI.GOV)
Not Available
2012-01-01
This technical highlight describes NREL research to develop Building Energy Simulation Test for Existing Homes (BESTEST-EX) to increase the quality and accuracy of energy analysis tools for the building retrofit market. Researchers at the National Renewable Energy Laboratory (NREL) have developed a new test procedure to increase the quality and accuracy of energy analysis tools for the building retrofit market. The Building Energy Simulation Test for Existing Homes (BESTEST-EX) is a test procedure that enables software developers to evaluate the performance of their audit tools in modeling energy use and savings in existing homes when utility bills are available formore » model calibration. Similar to NREL's previous energy analysis tests, such as HERS BESTEST and other BESTEST suites included in ANSI/ASHRAE Standard 140, BESTEST-EX compares software simulation findings to reference results generated with state-of-the-art simulation tools such as EnergyPlus, SUNREL, and DOE-2.1E. The BESTEST-EX methodology: (1) Tests software predictions of retrofit energy savings in existing homes; (2) Ensures building physics calculations and utility bill calibration procedures perform to a minimum standard; and (3) Quantifies impacts of uncertainties in input audit data and occupant behavior. BESTEST-EX includes building physics and utility bill calibration test cases. The diagram illustrates the utility bill calibration test cases. Participants are given input ranges and synthetic utility bills. Software tools use the utility bills to calibrate key model inputs and predict energy savings for the retrofit cases. Participant energy savings predictions using calibrated models are compared to NREL predictions using state-of-the-art building energy simulation programs.« less
32. Historic view of Building 202 test stand A with ...
32. Historic view of Building 202 test stand A with rocket engine, close-up detail of engine, November 19, 1957. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-46492. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
12. Historic view of Building 100 control room, showing television ...
12. Historic view of Building 100 control room, showing television monitoring of tests and personnel operating rocket engine test controls. May 27, 1957. On file at NASA Plumbrook Research Facility, Sandusky, Ohio. NASA photo number C-45021. - Rocket Engine Testing Facility, GRC Building No. 100, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
Criterion-Referenced Test (CRT) Items for Building Trades.
ERIC Educational Resources Information Center
Davis, Diane, Ed.
This test item bank is intended to help instructors construct criterion-referenced tests for secondary-level courses in building trades. The bank is keyed to the Missouri Building Trades Competency Profile, which was developed by industry and education professionals in Missouri, and is designed to be used in conjunction with the Vocational…
Technical Highlight: NREL Improves Building Energy Simulation Programs Through Diagnostic Testing
DOE Office of Scientific and Technical Information (OSTI.GOV)
Polly, B.
2012-01-09
This technical highlight describes NREL research to develop Building Energy Simulation Test for Existing Homes (BESTEST-EX) to increase the quality and accuracy of energy analysis tools for the building retrofit market.
Engineering biological systems using automated biofoundries
Chao, Ran; Mishra, Shekhar; Si, Tong; Zhao, Huimin
2017-01-01
Engineered biological systems such as genetic circuits and microbial cell factories have promised to solve many challenges in the modern society. However, the artisanal processes of research and development are slow, expensive, and inconsistent, representing a major obstacle in biotechnology and bioengineering. In recent years, biological foundries or biofoundries have been developed to automate design-build-test engineering cycles in an effort to accelerate these processes. This review summarizes the enabling technologies for such biofoundries as well as their early successes and remaining challenges. PMID:28602523
1984-03-22
BASIC EMISSIONS ESTIMAED BASED ON NAVAL AIR PROPULSION CENTER FUEL FLOW AND EMISSION FACTORS Time In Fuel Use Fuel Use Emission Factor NOX Power Node... power mode using smis- sions factors. Also accepted was that Lemoore would limit emissions of NOX from the test cells to 200 pounds per day. The...limits. In particular, the Main Stem and Power Plant, Building 174, exceeded air standards by approxi- mately 60 percent, and the Salvage Fuel Fired Boiler
Controlled Atmosphere High Temperature SPM for electrochemical measurements
NASA Astrophysics Data System (ADS)
Vels Hansen, K.; Sander, C.; Koch, S.; Mogensen, M.
2007-03-01
A new controlled atmosphere high temperature SPM has been designed and build for the purpose of performing electrochemical measurements on solid oxide fuel cell materials. The first tests show that images can be obtained at a surface temperature of 465°C in air with a standard AFM AC probe. The aim is to produce images at a surface temperature of 800°C with electrically conducting ceramic probes as working electrodes that can be positioned at desired locations at the surface for electrochemical measurements.
Disc size regulation in the brood cell building behavior of leaf-cutter bee, Megachile tsurugensis.
Kim, Jong-yoon
2007-12-01
The leaf-cutter bee, Megachile tsurugensis, builds a brood cell in a preexisting tunnel with leaf discs that she cuts in decreasing sizes and assembles them like a Russian matryoshka doll. By experimentally manipulating the brood cell, it was investigated how she regulates the size of leaf discs that fit in the brood cell's internal volume. When the internal volume was artificially increased by removing a bulk of leaf discs, she decreased the leaf disc size, although increasing it would have made the leaf disc more fitting in the increased internal volume. As a reverse manipulation, when the internal volume was decreased by inserting a group of inner layers of preassembled leaf discs to a brood cell, she decreased the leaf disc size, so that the leaf disc could fit in the decreased internal volume. These results suggest that she uses at least two different mechanisms to regulate the disc size: the use of some internal memory about the degree of building work accomplished in the first and of sensory feedback of dimensional information at the construction site in the second manipulation, respectively. It was concluded that a stigmergic mechanism, an immediate sensory feedback from the brood cell changed by the building work, alone cannot explain the details of the bee's behavior particularly with respect to her initial response to the first manipulation. For a more complete explanation of the behavior exhibited by the solitary bee, two additional behavioral elements, reinforcement of building activity and processing of dimensional information, were discussed along with stigmergy.
Magnetically controllable 3D microtissues based on magnetic microcryogels.
Liu, Wei; Li, Yaqian; Feng, Siyu; Ning, Jia; Wang, Jingyu; Gou, Maling; Chen, Huijun; Xu, Feng; Du, Yanan
2014-08-07
Microtissues on the scale of several hundred microns are a promising cell culture configuration resembling the functional tissue units in vivo. In contrast to conventional cell culture, handling of microtissues poses new challenges such as medium exchange, purification and maintenance of the microtissue integrity. Here, we developed magnetic microcryogels to assist microtissue formation with enhanced controllability and robustness. The magnetic microcryogels were fabricated on-chip by cryogelation and micro-molding which could endure extensive external forces such as fluidic shear stress during pipetting and syringe injection. The magnetically controllable microtissues were applied to constitute a novel separable 3D co-culture system realizing functional enhancement of the hepatic microtissues co-cultured with the stromal microtissues and easy purification of the hepatic microtissues for downstream drug testing. The magnetically controllable microtissues with pre-defined shapes were also applied as building blocks to accelerate the tissue assembly process under magnetic force for bottom-up tissue engineering. Finally, the magnetic microcryogels could be injected in vivo as cell delivery vehicles and tracked by MRI. The injectable magnetic microtissues maintained viability at the injection site indicating good retention and potential applications for cell therapy. The magnetic microcryogels are expected to significantly promote the microtissues as a promising cellular configuration for cell-based applications such as in drug testing, tissue engineering and regenerative therapy.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Ullrich, Rebecca A.
Assembly Building 9B (Building 09-54) is a contributing element to the Sandia National Laboratories (SNL) Tonopah Test Range (TTR) Historic District. The SNL TTR Historic District played a significant role in U.S. Cold War history in the areas of stockpile surveillance and non-nuclear field testing of nuclear weapons designs. The district covers approximately 179,200 acres and illustrates Cold War development testing of nuclear weapons components and systems. This report includes historical information, architectural information, sources of information, project information, maps, blueprints, and photographs.
Dong, Zuoli; Zhang, Naiqian; Li, Chun; Wang, Haiyun; Fang, Yun; Wang, Jun; Zheng, Xiaoqi
2015-06-30
An enduring challenge in personalized medicine is to select right drug for individual patients. Testing drugs on patients in large clinical trials is one way to assess their efficacy and toxicity, but it is impractical to test hundreds of drugs currently under development. Therefore the preclinical prediction model is highly expected as it enables prediction of drug response to hundreds of cell lines in parallel. Recently, two large-scale pharmacogenomic studies screened multiple anticancer drugs on over 1000 cell lines in an effort to elucidate the response mechanism of anticancer drugs. To this aim, we here used gene expression features and drug sensitivity data in Cancer Cell Line Encyclopedia (CCLE) to build a predictor based on Support Vector Machine (SVM) and a recursive feature selection tool. Robustness of our model was validated by cross-validation and an independent dataset, the Cancer Genome Project (CGP). Our model achieved good cross validation performance for most drugs in the Cancer Cell Line Encyclopedia (≥80% accuracy for 10 drugs, ≥75% accuracy for 19 drugs). Independent tests on eleven common drugs between CCLE and CGP achieved satisfactory performance for three of them, i.e., AZD6244, Erlotinib and PD-0325901, using expression levels of only twelve, six and seven genes, respectively. These results suggest that drug response could be effectively predicted from genomic features. Our model could be applied to predict drug response for some certain drugs and potentially play a complementary role in personalized medicine.
Constructing Aligned Assessments Using Automated Test Construction
ERIC Educational Resources Information Center
Porter, Andrew; Polikoff, Morgan S.; Barghaus, Katherine M.; Yang, Rui
2013-01-01
We describe an innovative automated test construction algorithm for building aligned achievement tests. By incorporating the algorithm into the test construction process, along with other test construction procedures for building reliable and unbiased assessments, the result is much more valid tests than result from current test construction…
Using a million cell simulation of the cerebellum: network scaling and task generality.
Li, Wen-Ke; Hausknecht, Matthew J; Stone, Peter; Mauk, Michael D
2013-11-01
Several factors combine to make it feasible to build computer simulations of the cerebellum and to test them in biologically realistic ways. These simulations can be used to help understand the computational contributions of various cerebellar components, including the relevance of the enormous number of neurons in the granule cell layer. In previous work we have used a simulation containing 12000 granule cells to develop new predictions and to account for various aspects of eyelid conditioning, a form of motor learning mediated by the cerebellum. Here we demonstrate the feasibility of scaling up this simulation to over one million granule cells using parallel graphics processing unit (GPU) technology. We observe that this increase in number of granule cells requires only twice the execution time of the smaller simulation on the GPU. We demonstrate that this simulation, like its smaller predecessor, can emulate certain basic features of conditioned eyelid responses, with a slight improvement in performance in one measure. We also use this simulation to examine the generality of the computation properties that we have derived from studying eyelid conditioning. We demonstrate that this scaled up simulation can learn a high level of performance in a classic machine learning task, the cart-pole balancing task. These results suggest that this parallel GPU technology can be used to build very large-scale simulations whose connectivity ratios match those of the real cerebellum and that these simulations can be used guide future studies on cerebellar mediated tasks and on machine learning problems. Copyright © 2012 Elsevier Ltd. All rights reserved.
Lenz, J; Linke, S; Gemein, S; Exner, M; Gebel, J
2010-06-01
Previous investigations of biofilms, generated in a silicone tube model have shown that the number of colony forming units (CFU) can reach 10(7)/cm(2), the total cell count (TCC) of microorganisms can be up to 10(8)cells/cm(2). The present study focuses on the situation in in-building distribution systems. Different chemical disinfectants were tested for their efficacy on drinking water biofilms in silicone tubes: free chlorine (electrochemically activated), chlorine dioxide, hydrogen peroxide (H(2)O(2)), silver, and fruit acids. With regard to the widely differing manufacturers' instructions for the usage of their disinfectants three different variations of the silicone tube model were developed to simulate practical use conditions. First the continuous treatment, second the intermittent treatment, third the efficacy of external disinfection treatment and the monitoring for possible biofilm formation with the Hygiene-Monitor. The working experience showed that it is important to know how to handle the individual disinfectants. Every active ingredient has its own optimal application concerning its concentration, exposure time, physical parameters like pH, temperature or redox potential. When used correctly all products tested were able to reduce the CFU to a value below the detection limit. Most of the active ingredients could not significantly reduce the TCC/cm(2), which means that viable microorganisms may still be present in the system. Thus the question arises what happened with these cells? In some cases SEM pictures of the biofilm matrix after a successful disinfection still showed biofilm residues. According to these results, no general correlation between CFU/cm(2), TCC/cm(2) and the visualised biofilm matrix on the silicone tube surface (SEM) could be demonstrated after a treatment with disinfectants. Copyright 2010 Elsevier GmbH. All rights reserved.
LOFT. Mobile test building (TAN624) is recycled from ANP program ...
LOFT. Mobile test building (TAN-624) is recycled from ANP program for placement before LOFT containment building door. It has not yet been connected to containment building. Note borated water tank at right of dome. Narrow, vertical structure at right of door is shroud is shroud for air exhaust duct. Filter vaults lie between duct shroud and stack. Camera facing westerly. Date: 1974. INEEL negative no. 74-1072 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID
LOFT. Containment building entry, an adapted use of TAN624, which ...
LOFT. Containment building entry, an adapted use of TAN-624, which originated as the mobile test building for the ANP program. Camera facing north. Note four-rail track entered building stack at right of view. Date: March 2004. INEEL negative no. HD-39-4-1 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID
Build an Inexpensive Wind Tunnel to Test CO2 Cars
ERIC Educational Resources Information Center
McCormick, Kevin
2012-01-01
As part of the technology education curriculum, the author's eighth-grade students design, build, test, and race CO2 vehicles. To help them in refining their designs, they use a wind tunnel to test for aerodynamic drag. In this article, the author describes how to build a wind tunnel using inexpensive, readily available materials. (Contains 1…
Ye, Yong; Li, Yue; Fang, Fei
2014-05-05
Cobra neurotoxin (NT) has central analgesic effects, but it is difficult to pass through brain blood barrier (BBB). A novel method of red light induction is designed to help NT across BBB, which is based on photosensitizer activation by red light to generate reactive oxygen species (ROS) to open BBB. The effects were evaluated on cell models and animals in vivo with illumination by semiconductor laser at 670nm on photosensitizer pheophorbide isolated from silkworm excrement. Brain microvascular endothelial cells and astrocytes were co-cultured to build up BBB cell model. The radioactivity of (125)I-NT was measured in cells and tissues for NT permeation. Three ways of cranial irradiation, nasal cavity and intravascular irradiation were tested with combined injection of (125)I-NT 20μg/kg and pheophorbide 100μg/kg to rats, and organs of rats were separated and determined the radioactivity. Paw pressure test in rats, hot plate and writhing test in mice were applied to appraise the analgesic effects. NT across BBB cell model increased with time of illumination, and reached stable level after 60min. So did ROS in cells. NT mainly distributed in liver and kidney of rats, significantly increased in brain after illumination, and improved analgesic effects. Excitation of pheophorbide at red light produces ROS to open BBB, help NT enter brain, and enhance its central action. This research provides a new method for drug across BBB to improve its central role. Copyright © 2014 Elsevier B.V. All rights reserved.
112. ARAI Hot cell (ARA626) Building roof plan and details ...
112. ARA-I Hot cell (ARA-626) Building roof plan and details of roof ventilating equipment and parapet. Norman Engineering Company: 961-area/SF-626-A-2. Date: January 1959. Ineel index code no. 068-0626-00-613-102722. - Idaho National Engineering Laboratory, Army Reactors Experimental Area, Scoville, Butte County, ID
NASA Researcher Adjusts a Travelling Magnetic Wave Plasma Engine
1964-02-21
Raymond Palmer, of the Electromagnetic Propulsion Division’s Plasma Flow Section, adjusts the traveling magnetic wave plasma engine being operated in the Electric Power Conversion at the National Aeronautics and Space Administration (NASA) Lewis Research Center. During the 1960s Lewis researchers were exploring several different methods of creating electric propulsion systems, including the traveling magnetic wave plasma engine. The device operated similarly to alternating-current motors, except that a gas, not a solid, was used to conduct the electricity. A magnetic wave induced a current as it passed through the plasma. The current and magnetic field pushed the plasma in one direction. Palmer and colleague Robert Jones explored a variety of engine configurations in the Electric Propulsion Research Building. The engine is seen here mounted externally on the facility’s 5-foot diameter and 16-foot long vacuum tank. The four magnetic coils are seen on the left end of the engine. The researchers conducted two-minute test runs with varying configurations and used of both argon and xenon as the propellant. The Electric Propulsion Research Building was built in 1942 as the Engine Propeller Research Building, often called the Prop House. It contained four test cells to study large reciprocating engines with their propellers. After World War II, the facility was modified to study turbojet engines. By the 1960s, the facility was modified again for electric propulsion research and given its current name.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Lacommare, Kristina S H; Stadler, Michael; Aki, Hirohisa
The addition of storage technologies such as flow batteries, conventional batteries, and heat storage can improve the economic as well as environmental attractiveness of on-site generation (e.g., PV, fuel cells, reciprocating engines or microturbines operating with or without CHP) and contribute to enhanced demand response. In order to examine the impact of storage technologies on demand response and carbon emissions, a microgrid's distributed energy resources (DER) adoption problem is formulated as a mixed-integer linear program that has the minimization of annual energy costs as its objective function. By implementing this approach in the General Algebraic Modeling System (GAMS), the problemmore » is solved for a given test year at representative customer sites, such as schools and nursing homes, to obtain not only the level of technology investment, but also the optimal hourly operating schedules. This paper focuses on analysis of storage technologies in DER optimization on a building level, with example applications for commercial buildings. Preliminary analysis indicates that storage technologies respond effectively to time-varying electricity prices, i.e., by charging batteries during periods of low electricity prices and discharging them during peak hours. The results also indicate that storage technologies significantly alter the residual load profile, which can contribute to lower carbon emissions depending on the test site, its load profile, and its adopted DER technologies.« less
Lautenschlager, Karin; Boon, Nico; Wang, Yingying; Egli, Thomas; Hammes, Frederik
2010-09-01
Drinking water quality is routinely monitored in the distribution network but not inside households at the point of consumption. Fluctuating temperatures, residence times (stagnation), pipe materials and decreasing pipe diameters can promote bacterial growth in buildings. To test the influence of stagnation in households on the bacterial cell concentrations and composition, water was sampled from 10 separate households after overnight stagnation and after flushing the taps. Cell concentrations, measured by flow cytometry, increased (2-3-fold) in all water samples after stagnation. This increase was also observed in adenosine tri-phosphate (ATP) concentrations (2-18-fold) and heterotrophic plate counts (4-580-fold). An observed increase in cell biovolume and ATP-per-cell concentrations furthermore suggests that the increase in cell concentrations was due to microbial growth. After 5 min flushing of the taps, cell concentrations and water temperature decreased to the level generally found in the drinking water network. Denaturing gradient gel electrophoresis also showed a change in the microbial composition after stagnation. This study showed that water stagnation in household pipes results in considerable microbial changes. While hygienic risk was not directly assessed, it emphasizes the need for the development of good material validation methods, recommendations and spot tests for in-house water installations. However, a simple mitigation strategy would be a short flushing of taps prior to use. Copyright © 2010 Elsevier Ltd. All rights reserved.
Characteristics of Recycled Concrete Aggregates from Precast Slab Block Buildings
NASA Astrophysics Data System (ADS)
Venkrbec, Václav; Nováková, Iveta; Henková, Svatava
2017-10-01
Precast slab block buildings (PSBB) typically and frequently occur in Central and Eastern Europe, as well as elsewhere in the world. Some of these buildings are currently used beyond their service life capacity. The utilization of recycled materials from these buildings with regard to applying the principles of sustainable construction and using recycled materials will probably be significant in the following years. Documentation from the manufacturing processes of prefabricated blocks for precast slab block buildings is not available, and also it is difficult to declare technological discipline during the construction of these buildings. Therefore, properties of recycled concrete aggregates (RCA) produced from construction and demolition waste (C&DW) of precast slab block buildings build between 1950s to 1990s are not sufficiently known. The demolition of these buildings is very rare today, but it can be assumed an increase in demolitions of these buildings in the future. The use of RCA in new concrete requires verification/testing of the geometrical and physical properties of RCA according to the EN 12 620+A1 standard. The aim of the contribution is to present a case study of the demolition of slab block building with emphasis on RCA usage. The paper presents the results of the tests according to European standards for determining selected geometrical and physical properties of the RCA. The paper describes and evaluates tests such as determination of particle size distribution - Sieve Analysis, content of fine particles, determination of density and water absorption. The results of the properties testing of RCA are compared with the properties of natural aggregate. The general boundary conditions of RCA particular tests are presented.
Eddy current sensor for in-situ monitoring of swelling of Li-ion prismatic cells
NASA Astrophysics Data System (ADS)
Plotnikov, Yuri; Karp, Jason; Knobloch, Aaron; Kapusta, Chris; Lin, David
2015-03-01
In-situ monitoring an on-board rechargeable battery in hybrid cars can be used to ensure a long operating life of the battery and safe operation of the vehicle. Intercalations of ions in the electrode material during charge and discharge of a Lithium Ion battery cause periodic stress and strain of the electrode materials that can ultimately lead to fatigue resulting in capacity loss and potential battery failure. Currently this process is not monitored directly on the cells. This work is focused on development technologies that would quantify battery swelling and provide in-situ monitoring for onboard vehicle applications. Several rounds of tests have been performed to spatially characterize cell expansion of a 5 Ah cell with a nickel/manganese/cobalt-oxide cathode (Sanyo, Japan) used by Ford in their Fusion HEV battery pack. A collaborative team of researchers from GE and the University of Michigan has characterized the free expansion of these cells to be in the range of 100×125 microns (1% of total cell thickness) at the center point of the cell. GE proposed to use a thin eddy current (EC) coil to monitor these expansions on the cells while inside the package. The photolithography manufacturing process previously developed for EC arrays for detecting cracks in aircraft engine components was used to build test coils for gap monitoring. These sensors are thin enough to be placed safely between neighboring cells and capable of monitoring small variations in the gap between the cells. Preliminary investigations showed that these coils can be less than 100 micron thick and have sufficient sensitivity in a range from 0 to 2 mm. Laboratory tests revealed good correlation between EC and optical gap measurements in the desired range. Further technology development could lead to establishing a sensor network for a low cost solution for the in-situ monitoring of cell swelling during battery operation.
ETR HEAT EXCHANGER BUILDING, TRA644. EAST SIDE. CAMERA FACING WEST. ...
ETR HEAT EXCHANGER BUILDING, TRA-644. EAST SIDE. CAMERA FACING WEST. NOTE COURSE OF PIPE FROM GROUND AND FOLLOWING ROOF OF BUILDING. MTR BUILDING IN BACKGROUND AT RIGHT EDGE OF VIEW. INL NEGATIVE NO. HD46-36-3. Mike Crane, Photographer, 4/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
114. ARAI Hot cell (ARA626) Building details of fuel storage ...
114. ARA-I Hot cell (ARA-626) Building details of fuel storage pit in plan and section. Spaces shown for 20 elements. Norman Engineering Company: 961-area/SF-626-S-4. Date: January 1959. Ineel index code no. 068-0626-60-613-102752. - Idaho National Engineering Laboratory, Army Reactors Experimental Area, Scoville, Butte County, ID
NASA Astrophysics Data System (ADS)
Zhou, Yong; Gao, Huai-Ling; Shen, Li-Li; Pan, Zhao; Mao, Li-Bo; Wu, Tao; He, Jia-Cai; Zou, Duo-Hong; Zhang, Zhi-Yuan; Yu, Shu-Hong
2015-12-01
Scaffolds for tissue engineering (TE) which closely mimic the physicochemical properties of the natural extracellular matrix (ECM) have been proven to advantageously favor cell attachment, proliferation, migration and new tissue formation. Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach. Nevertheless, micro-components with an ECM-mimicking nanofibrous structure are still very scarce and highly desirable. Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration. According to this background, we report here the fabrication of chitosan microspheres with an ECM-mimicking nanofibrous structure for the first time based on a physical gelation process. By combining this physical fabrication procedure with microfluidic technology, uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes can be facilely obtained. Especially, no potentially toxic or denaturizing chemical crosslinking agent was introduced into the products. Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering.Scaffolds for tissue engineering (TE) which closely mimic the physicochemical properties of the natural extracellular matrix (ECM) have been proven to advantageously favor cell attachment, proliferation, migration and new tissue formation. Recently, as a valuable alternative, a bottom-up TE approach utilizing cell-loaded micrometer-scale modular components as building blocks to reconstruct a new tissue in vitro or in vivo has been proved to demonstrate a number of desirable advantages compared with the traditional bulk scaffold based top-down TE approach. Nevertheless, micro-components with an ECM-mimicking nanofibrous structure are still very scarce and highly desirable. Chitosan (CS), an accessible natural polymer, has demonstrated appealing intrinsic properties and promising application potential for TE, especially the cartilage tissue regeneration. According to this background, we report here the fabrication of chitosan microspheres with an ECM-mimicking nanofibrous structure for the first time based on a physical gelation process. By combining this physical fabrication procedure with microfluidic technology, uniform CS microspheres (CMS) with controlled nanofibrous microstructure and tunable sizes can be facilely obtained. Especially, no potentially toxic or denaturizing chemical crosslinking agent was introduced into the products. Notably, in vitro chondrocyte culture tests revealed that enhanced cell attachment and proliferation were realized, and a macroscopic 3D geometrically shaped cartilage-like composite can be easily constructed with the nanofibrous CMS (NCMS) and chondrocytes, which demonstrate significant application potential of NCMS as the bottom-up cell-carrier components for cartilage tissue engineering. Electronic supplementary information (ESI) available: Additional figures and table. See DOI: 10.1039/c5nr06876b
Computer-aided design of biological circuits using TinkerCell.
Chandran, Deepak; Bergmann, Frank T; Sauro, Herbert M
2010-01-01
Synthetic biology is an engineering discipline that builds on modeling practices from systems biology and wet-lab techniques from genetic engineering. As synthetic biology advances, efficient procedures will be developed that will allow a synthetic biologist to design, analyze, and build biological networks. In this idealized pipeline, computer-aided design (CAD) is a necessary component. The role of a CAD application would be to allow efficient transition from a general design to a final product. TinkerCell is a design tool for serving this purpose in synthetic biology. In TinkerCell, users build biological networks using biological parts and modules. The network can be analyzed using one of several functions provided by TinkerCell or custom programs from third-party sources. Since best practices for modeling and constructing synthetic biology networks have not yet been established, TinkerCell is designed as a flexible and extensible application that can adjust itself to changes in the field. © 2010 Landes Bioscience
NASA Astrophysics Data System (ADS)
Alzoubi, Hussain Hendi
Energy consumption in buildings has recently become a major concern for environmental designers. Within this field, daylighting and solar energy design are attractive strategies for saving energy. This study seeks the integrity and the optimality of building envelopes' performance. It focuses on the transparent parts of building facades, specifically, the windows and their shading devices. It suggests a new automated method of utilizing solar energy while keeping optimal solutions for indoor daylighting. The method utilizes a statistical approach to produce mathematical equations based on physical experimentation. A full-scale mock-up representing an actual office was built. Heat gain and lighting levels were measured empirically and correlated with blind angles. Computational methods were used to estimate the power production from photovoltaic cells. Mathematical formulas were derived from the results of the experiments; these formulas were utilized to construct curves as well as mathematical equations for the purpose of optimization. The mathematical equations resulting from the optimization process were coded using Java programming language to enable future users to deal with generic locations of buildings with a broader context of various climatic conditions. For the purpose of optimization by automation under different climatic conditions, a blind control system was developed based on the findings of this study. This system calibrates the blind angles instantaneously based upon the sun position, the indoor daylight, and the power production from the photovoltaic cells. The functions of this system guarantee full control of the projected solar energy on buildings' facades for indoor lighting and heat gain. In winter, the system automatically blows heat into the space, whereas it expels heat from the space during the summer season. The study showed that the optimality of building facades' performance is achievable for integrated thermal, energy, and lighting models in buildings. There are blind angles that produce maximum energy from the photovoltaic cells while keeping indoor light within the acceptable limits that prevent undesired heat gain in summer.
2. Credit BG. Looking west at east facade of Steam ...
2. Credit BG. Looking west at east facade of Steam Generator Plant, Building 4280/E-81; steam generators have been removed as part of dismantling program for Test Stand 'D.' Metal cylindrical objects to left of door were roof vents. The steam-driven ejector system for Dv Cell is clearly visible on the east side of Test Stand 'D' tower. The X-stage ejector is vertically installed at the bottom left of the tower, Y-stage is horizontally positioned close to the tower top, and the Z- and Z-1 stages are attached to the top of the interstage condenser. Light-colored piping is thermally insulated steam line. - Jet Propulsion Laboratory Edwards Facility, Test Stand D, Steam Generator Plant, Edwards Air Force Base, Boron, Kern County, CA
Damage Assessment of a Full-Scale Six-Story wood-frame Building Following Triaxial shake Table Tests
John W. van de Lindt; Rakesh Gupta; Shiling Pei; Kazuki Tachibana; Yasuhiro Araki; Douglas Rammer; Hiroshi Isoda
2012-01-01
In the summer of 2009, a full-scale midrise wood-frame building was tested under a series of simulated earthquakes on the world's largest shake table in Miki City, Japan. The objective of this series of tests was to validate a performance-based seismic design approach by qualitatively and quantitatively examining the building's seismic performance in terms of...
Fuel cell commercialization — beyond the 'Notice of Market Opportunity for Fuel Cells' (NOMO)
NASA Astrophysics Data System (ADS)
Serfass, J. A.; Glenn, D. R.
1992-01-01
The Notice of Market Opportunity for Fuel Cells (NOMO) was released in Oct. 1988 by the American Public Power Association. Its goal was to identify a manufacturer for commercializing a multi-megawatt fuel cell power plant with attractive cost and performance characteristics, supported by a realistic, yet aggressive commercialization plan, leading to mid-1990s application. Energy Research Corporation's program to commercialize its 2-MW internal-reforming carbonate fuel cell was selected. The program was refined in the development of the Principles and Framework for Commercializing Direct Fuel Cell Power Plants, which defines buyer responsibilities for promotion and coordination of information development, supplier responsibilities for meeting certain milestones and for sharing the results of success in a royalty agreement, and risk management features. Twenty-three electric and gas utilities in the US and Canada have joined the Fuel Cell Commercialization Group to support the buyers' obligations in this program. The City of Santa Clara, CA; Electric Power Research Institute; Los Angeles Department of Water and Power; Southern California Gas Company; Southern California Edison; National Rural Electric Cooperative Association; and Pacific Gas & Electric, have formed the Santa Clara Demonstration Group to build the first 2-MW power plant. The preliminary design for this demonstration is nearly complete. Integrated testing of a 20-kW stack with the complete balance-of-plant, has been successfully accomplished by Pacific Gas & Electric at its test facility in San Ramon, CA.
46. ARAI. Aerial view of ARAI buildings as they looked ...
46. ARA-I. Aerial view of ARA-I buildings as they looked in 1981. From left to right, buildings are tank (ARA-727), contaminated waste storage tank (ARA-629), trailer, hot cell building (ARA-626), fuel oil storage tank (ARA-728), guard house (ARA-628), shop and maintenance building (ARA-627), and two trailers. Ineel photo no. 81-297. - Idaho National Engineering Laboratory, Army Reactors Experimental Area, Scoville, Butte County, ID
Testing Method for External Cladding Systems - Incerc Romania
NASA Astrophysics Data System (ADS)
Simion, A.; Dragne, H.
2017-06-01
This research presents a new testing method in a natural scale for external cladding systems tested on buildings with minimum than 3 floors [1]. The testing method is unique in Romania and it is similar about many fire testing current methods from European Union states. Also, presents the fire propagation and the effect of fire smoke on the building façade composed of thermal insulation. Laboratory of testing and research for building fire safety from National Institute INCERC Bucharest, provides a test method for determining the fire performance characteristics of non-loadbearing external cladding systems and external wall insulation systems when applied to the face of a building and exposed to an external fire under controlled conditions [2]. The fire exposure is representative of an external fire source or a fully-developed (post-flashover) fire in a room, venting through an opening such as a window aperture that exposes the cladding to the effects of external flames, or an external fire source. On the future, fire tests will be experimented for answer demande a number of high-profile fires where the external facade of tall buildings provided a route for vertical fire spread.
Alizadeh Sardroud, Hamed; Nemati, Sorour; Baradar Khoshfetrat, Ali; Nabavinia, Mahbobeh; Beygi Khosrowshahi, Younes
2017-08-01
Influence of gelatine concentration and cross-linker ions of Ca 2+ and Ba 2+ was evaluated on characteristics of alginate hydrogels and proliferation behaviours of model adherent and suspendable stem cells of fibroblast and U937 embedded in alginate microcapsules. Increasing gelatine concentration to 2.5% increased extent of swelling to 15% and 25% for barium- and calcium-cross-linked hydrogels, respectively. Mechanical properties also decreased with increasing swelling of hydrogels. Both by increasing gelatine concentration and using barium ions increased considerably the proliferation of encapsulated model stem cells. Barium-cross-linked alginate-gelatine microcapsule tested for bone building block showed a 13.5 ± 1.5-fold expansion for osteoblast cells after 21 days with deposition of bone matrix. The haematopoietic stem cells cultured in the microcapsule after 7 days also showed up to 2-fold increase without adding any growth factor. The study demonstrates that barium-cross-linked alginate-gelatine microcapsule has potential for use as a simple and efficient 3D platform for stem cell production and modular tissue formation.
Investigation of test methods, material properties, and processes for solar cell encapsulants
NASA Technical Reports Server (NTRS)
Willis, P. B.
1985-01-01
Progress in solar energy technology is reported in the following areas: aging and life prediction methodology and devices for solar cell encapsulation; the function of adhesion chemistry, primers, and a new diagnostic technique for estimations of bond durability; a study of fire retardant formulations for decreasing the potential flammability of solar modules; initial studies of the electrical insulating properties of encapsulation materials and measurement of the intrinsic dielectric strength; antisoiling compounds for the prevention of soil build-up on the outer surface of the module; and low temperature processing encapsulants that permit module fabrication at temperatures less than 100 C. Another area of study has been added to determine the degree to which formulation and processes affect the module quality and manufacturing yield.
Microbial fuel cells for biosensor applications.
Yang, Huijia; Zhou, Minghua; Liu, Mengmeng; Yang, Weilu; Gu, Tingyue
2015-12-01
Microbial fuel cells (MFCs) face major hurdles for real-world applications as power generators with the exception of powering small sensor devices. Despite tremendous improvements made in the last two decades, MFCs are still too expensive to build and operate and their power output is still too small. In view of this, in recently years, intensive researches have been carried out to expand the applications into other areas such as acid and alkali production, bioremediation of aquatic sediments, desalination and biosensors. Unlike power applications, MFC sensors have the immediate prospect to be practical. This review covers the latest developments in various proposed biosensor applications using MFCs including monitoring microbial activity, testing biochemical oxygen demand, detection of toxicants and detection of microbial biofilms that cause biocorrosion.
Gray, Michael R; Thrasher, Jack D; Crago, Robert; Madison, Roberta A; Arnold, Linda; Campbell, Andrew W; Vojdani, Aristo
2003-07-01
The study described was part of a larger multicenter investigation of patients with multiple health complaints attributable to confirmed exposure to mixed-molds infestation in water-damaged buildings. The authors present data on symptoms; clinical chemistries; abnormalities in pulmonary function; alterations in T, B, and natural killer (NK) cells; the presence of autoantibodies (i.e., antinuclear autoantibodies [ANA], autoantibodies against smooth muscle [ASM], and autoantibodies against central nervous system [CNS] and peripheral nervous system [PNS] myelins). A total of 209 adults, 42.7 +/- 16 yr of age (mean +/- standard deviation), were examined and tested with (a) self-administered weighted health history and symptom questionnaires; (b) standardized physical examinations; (c) complete blood counts and blood and urine chemistries; (d) urine and fecal cultures; (e) thyroid function tests (T4, free T3); (f) pulmonary function tests (forced vital capacity [FVC], forced expiratory volume in 1 sec [FEV1.0], and forced expiratory flow at 25%, 50%, 75%, and 25-75% of FVC [FEF25, FEF50, FEF75, and FEF2(25-75)]); (g) peripheral lymphocyte phenotypes (T, B, and NK cells) and mitogenesis determinations; and (h) a 13-item autoimmune panel. The molds-exposed patients reported a greater frequency and intensity of symptoms, particularly neurological and inflammatory symptoms, when compared with controls. The percentages of exposed individuals with increased lymphocyte phenotypes were: B cells (CD20+), 75.6%; CD5+CD25+, 68.9%; CD3+CD26+, 91.2%; CD8+HLR-DR+, 62%; and CD8+CD38+, 56.6%; whereas other phenotypes were decreased: CD8+CD11b+, 15.6% and CD3-CD16+CD56+, 38.5%. Mitogenesis to phytohemagglutinin was decreased in 26.2% of the exposed patients, but only 5.9% had decreased response to concanavalin A. Abnormally high levels of ANA, ASM, and CNS myelin (immunoglobulins [Ig]G, IgM, IgA) and PNS myelin (IgG, IgM, IgA) were found; odds ratios for each were significant at 95% confidence intervals, showing an increased risk for autoimmunity. The authors conclude that exposure to mixed molds and their associated mycotoxins in water-damaged buildings leads to multiple health problems involving the CNS and the immune system, in addition to pulmonary effects and allergies. Mold exposure also initiates inflammatory processes. The authors propose the term "mixed mold mycotoxicosis" for the multisystem illness observed in these patients.
Building a Better Mousetrap Offers Cross-Curricular Connections
ERIC Educational Resources Information Center
Barton, Richard
2012-01-01
"If you build a better mousetrap, the world will beat a path to your door," Ralph Waldo Emerson is often credited with saying. In today's world, that might more likely be "if you build a better cell phone..." or maybe "a better vehicle..." But as an 8th-grade design project, the author challenges his students to build that better mousetrap. In…
Judson, Richard S; Houck, Keith A; Kavlock, Robert J; Knudsen, Thomas B; Martin, Matthew T; Mortensen, Holly M; Reif, David M; Rotroff, Daniel M; Shah, Imran; Richard, Ann M; Dix, David J
2010-04-01
Chemical toxicity testing is being transformed by advances in biology and computer modeling, concerns over animal use, and the thousands of environmental chemicals lacking toxicity data. The U.S. Environmental Protection Agency's ToxCast program aims to address these concerns by screening and prioritizing chemicals for potential human toxicity using in vitro assays and in silico approaches. This project aims to evaluate the use of in vitro assays for understanding the types of molecular and pathway perturbations caused by environmental chemicals and to build initial prioritization models of in vivo toxicity. We tested 309 mostly pesticide active chemicals in 467 assays across nine technologies, including high-throughput cell-free assays and cell-based assays, in multiple human primary cells and cell lines plus rat primary hepatocytes. Both individual and composite scores for effects on genes and pathways were analyzed. Chemicals displayed a broad spectrum of activity at the molecular and pathway levels. We saw many expected interactions, including endocrine and xenobiotic metabolism enzyme activity. Chemicals ranged in promiscuity across pathways, from no activity to affecting dozens of pathways. We found a statistically significant inverse association between the number of pathways perturbed by a chemical at low in vitro concentrations and the lowest in vivo dose at which a chemical causes toxicity. We also found associations between a small set of in vitro assays and rodent liver lesion formation. This approach promises to provide meaningful data on the thousands of untested environmental chemicals and to guide targeted testing of environmental contaminants.
Wright R–2600–8 Engine in the Engine Propeller Research Building
1943-03-21
A Wright Aeronautical R–2600 Cyclone piston engine installed in the Engine Propeller Research Building, or Prop House, at the National Advisory Committee for Aeronautics (NACA) Aircraft Engine Research Laboratory. The R–2600 was among the most powerful engines that emerged during World War II. The engine, which was developed for commercial applications in 1939, was used to power the North American B–25 bomber and several other midsize military aircraft. The higher altitudes required by the military caused problems with the engine's cooling and fuel systems. The military requested that the Aircraft Engine Research Laboratory analyze the performance of the R–2600, improve its cooling system, and reduce engine knock. The NACA researchers subjected the engine to numerous tests in its Prop House. The R–2600 was the subject of the laboratory's first technical report, which was written by members of the Fuels and Lubricants Division. The Prop House contained soundproof test cells in which piston engines and propellers were mounted and operated at high powers. Electrically driven fans drew air through ducts to create a stream of cooling air over the engines. Researchers tested the performance of fuels, turbochargers, water-injection and cooling systems here during World War II. The facility was also investigated a captured German V–I buzz bomb during the war.
Toxicity of airborne dust as an indicator of moisture problems in school buildings.
Tirkkonen, Jenni; Täubel, Martin; Leppänen, Hanna; Peltonen, Matti; Lindsley, William; Chen, Bean T; Hyvärinen, Anne; Hirvonen, Maija-Riitta; Huttunen, Kati
2017-02-01
Moisture-damaged indoor environments are thought to increase the toxicity of indoor air particulate matter (PM), indicating that a toxicological assay could be used as a method for recognizing buildings with indoor air problems. We aimed to test if our approach of analyzing the toxicity of actively collected indoor air PM in vitro differentiates moisture-damaged from non-damaged school buildings. We collected active air samples with NIOSH Bioaerosol Cyclone Samplers from moisture-damaged (index) and non-damaged (reference) school buildings (4 + 4). The teachers and pupils of the schools were administered a symptom questionnaire. Five samples of two size fractions [Stage 1 (>1.9 μm) and Stage 2 (1-1.9 μm)] were collected from each school. Mouse RAW264.7 macrophages were exposed to the collected PM for 24 h and subsequently analyzed for changes in cell metabolic activity, production of nitric oxide (NO), tumor necrosis factor (TNF)-α and interleukin (IL)-6. The teachers working in the moisture-damaged schools reported respiratory symptoms such as cough (p = 0.01) and shortness of breath (p = 0.01) more often than teachers from reference schools. Toxicity of the PM sample as such did not differentiate index from reference building,s but the toxicity adjusted for the amount of the particles tended to be higher in moisture-damaged schools. Further development of the method will require identification of other confounding factors in addition to the necessity to adjust for differences in particle counts between samples.
12. SOUTHWEST VIEW OF BUILDING 25C (SUBSONIC AERODYNAMICS TEST FACILITY) ...
12. SOUTHWEST VIEW OF BUILDING 25C (SUBSONIC AERODYNAMICS TEST FACILITY) (1992). - Wright-Patterson Air Force Base, Area B, Buildings 25 & 24,10-foot & 20-foot Wind Tunnel Complex, Northeast side of block bounded by K, G, Third, & Fifth Streets, Dayton, Montgomery County, OH
Prior to re-entering a building following a chemical attack, decontamination and testing must be conducted to determine whether toxic agents have been eliminated or reduced to safe levels. Building contents must also be decontaminated and tested or destroyed. Recent incidents i...
Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate.
Shibata, Tomonori; Fujita, Yoshihiko; Ohno, Hirohisa; Suzuki, Yuki; Hayashi, Karin; Komatsu, Kaoru R; Kawasaki, Shunsuke; Hidaka, Kumi; Yonehara, Shin; Sugiyama, Hiroshi; Endo, Masayuki; Saito, Hirohide
2017-09-14
Nucleic acid nanotechnology has great potential for future therapeutic applications. However, the construction of nanostructured devices that control cell fate by detecting and amplifying protein signals has remained a challenge. Here we design and build protein-driven RNA-nanostructured devices that actuate in vitro by RNA-binding-protein-inducible conformational change and regulate mammalian cell fate by RNA-protein interaction-mediated protein assembly. The conformation and function of the RNA nanostructures are dynamically controlled by RNA-binding protein signals. The protein-responsive RNA nanodevices are constructed inside cells using RNA-only delivery, which may provide a safe tool for building functional RNA-protein nanostructures. Moreover, the designed RNA scaffolds that control the assembly and oligomerization of apoptosis-regulatory proteins on a nanometre scale selectively kill target cells via specific RNA-protein interactions. These findings suggest that synthetic RNA nanodevices could function as molecular robots that detect signals and localize target proteins, induce RNA conformational changes, and programme mammalian cellular behaviour.Nucleic acid nanotechnology has great potential for future therapeutic applications. Here the authors build protein-driven RNA nanostructures that can function within mammalian cells and regulate the cell fate.
Sun, Hongwei; Li, Guiying; Nie, Xin; Shi, Huixian; Wong, Po-Keung; Zhao, Huijun; An, Taicheng
2014-08-19
A systematic approach was developed to understand, in-depth, the mechanisms involved during the inactivation of bacterial cells using photoelectrocatalytic (PEC) processes with Escherichia coli K-12 as the model microorganism. The bacterial cells were found to be inactivated and decomposed primarily due to attack from photogenerated H2O2. Extracellular reactive oxygen species (ROSs), such as H2O2, may penetrate into the bacterial cell and cause dramatically elevated intracellular ROSs levels, which would overwhelm the antioxidative capacity of bacterial protective enzymes such as superoxide dismutase and catalase. The activities of these two enzymes were found to decrease due to the ROSs attacks during PEC inactivation. Bacterial cell wall damage was then observed, including loss of cell membrane integrity and increased permeability, followed by the decomposition of cell envelope (demonstrated by scanning electronic microscope images). One of the bacterial building blocks, protein, was found to be oxidatively damaged due to the ROSs attacks, as well. Leakage of cytoplasm and biomolecules (bacterial building blocks such as proteins and nucleic acids) were evident during prolonged PEC inactivation process. The leaked cytoplasmic substances and cell debris could be further degraded and, ultimately, mineralized with prolonged PEC treatment.
9. BUILDING 8769, EAST REAR AND NORTH SIDE, TEST STAND ...
9. BUILDING 8769, EAST REAR AND NORTH SIDE, TEST STAND AT RIGHT. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Observation Bunkers for Test Stand 1-A, Test Area 1-120, north end of Jupiter Boulevard, Boron, Kern County, CA
Disc size regulation in the brood cell building behavior of leaf-cutter bee, Megachile tsurugensis
NASA Astrophysics Data System (ADS)
Kim, Jong-Yoon
2007-12-01
The leaf-cutter bee, Megachile tsurugensis, builds a brood cell in a preexisting tunnel with leaf discs that she cuts in decreasing sizes and assembles them like a Russian matryoshka doll. By experimentally manipulating the brood cell, it was investigated how she regulates the size of leaf discs that fit in the brood cell’s internal volume. When the internal volume was artificially increased by removing a bulk of leaf discs, she decreased the leaf disc size, although increasing it would have made the leaf disc more fitting in the increased internal volume. As a reverse manipulation, when the internal volume was decreased by inserting a group of inner layers of preassembled leaf discs to a brood cell, she decreased the leaf disc size, so that the leaf disc could fit in the decreased internal volume. These results suggest that she uses at least two different mechanisms to regulate the disc size: the use of some internal memory about the degree of building work accomplished in the first and of sensory feedback of dimensional information at the construction site in the second manipulation, respectively. It was concluded that a stigmergic mechanism, an immediate sensory feedback from the brood cell changed by the building work, alone cannot explain the details of the bee’s behavior particularly with respect to her initial response to the first manipulation. For a more complete explanation of the behavior exhibited by the solitary bee, two additional behavioral elements, reinforcement of building activity and processing of dimensional information, were discussed along with stigmergy.
Experimental and Analytical Evaluation of a Composite Honeycomb Deployable Energy Absorber
NASA Technical Reports Server (NTRS)
Jackson, Karen E.; Kellas, Sotiris; Horta, Lucas G.; Annett, Martin S.; Polanco, Michael A.; Littell, Justin D.; Fasanella, Edwin L.
2011-01-01
In 2006, the NASA Subsonic Rotary Wing Aeronautics Program sponsored the experimental and analytical evaluation of an externally deployable composite honeycomb structure that is designed to attenuate impact energy during helicopter crashes. The concept, which is designated the Deployable Energy Absorber (DEA), utilizes an expandable Kevlar honeycomb structure to dissipate kinetic energy through crushing. The DEA incorporates a unique flexible hinge design that allows the honeycomb to be packaged and stowed flat until needed for deployment. A variety of deployment options such as linear, radial, and/or hybrid methods can be used. Experimental evaluation of the DEA utilized a building block approach that included material characterization testing of its constituent, Kevlar -129 fabric/epoxy, and flexural testing of single hexagonal cells. In addition, the energy attenuation capabilities of the DEA were demonstrated through multi-cell component dynamic crush tests, and vertical drop tests of a composite fuselage section, retrofitted with DEA blocks, onto concrete, water, and soft soil. During each stage of the DEA evaluation process, finite element models of the test articles were developed and simulations were performed using the explicit, nonlinear transient dynamic finite element code, LS-DYNA. This report documents the results of the experimental evaluation that was conducted to assess the energy absorption capabilities of the DEA.
2014-06-06
CAPE CANAVERAL, Fla. -- Inside the Operations and Checkout Building high bay at NASA's Kennedy Space Center in Florida, NASA and Lockheed Martin engineers and technicians monitor the progress as a crane lowers the Orion service module into the Final Assembly and System Testing, or FAST, cell. The Orion crew module will be stacked on the service module in the FAST cell and then both modules will be put through their final system tests for Exploration Flight Test-1, or EFT-1, before rolling out of the facility for integration with the United Launch Alliance Delta IV Heavy rocket. Orion is the exploration spacecraft designed to carry astronauts to destinations not yet explored by humans, including an asteroid and Mars. It will have emergency abort capability, sustain the crew during space travel and provide safe re-entry from deep space return velocities. The first unpiloted test flight of Orion, EFT-1, is scheduled to launch later this year atop a Delta IV rocket from Cape Canaveral Air Force Station in Florida to an altitude of 3,600 miles above the Earth's surface. The two-orbit, four-hour flight test will help engineers evaluate the systems critical to crew safety including the heat shield, parachute system and launch abort system. For more information, visit http://www.nasa.gov/orion. Photo credit: NASA/Glenn Benson
2014-06-06
CAPE CANAVERAL, Fla. -- Inside the Operations and Checkout Building high bay at NASA's Kennedy Space Center in Florida, NASA and Lockheed Martin technicians and engineers prepare to move the Orion service module to the Final Assembly and System Testing, or FAST, cell further down the aisle. The Orion crew module will be stacked on the service module in the FAST cell and then both modules will be put through their final system tests for Exploration Flight Test-1, or EFT-1, prior to rolling out of the facility for integration with the United Launch Alliance Delta IV Heavy rocket. Orion is the exploration spacecraft designed to carry astronauts to destinations not yet explored by humans, including an asteroid and Mars. It will have emergency abort capability, sustain the crew during space travel and provide safe re-entry from deep space return velocities. The first unpiloted test flight of Orion, EFT-1, is scheduled to launch later this year atop a Delta IV rocket from Cape Canaveral Air Force Station in Florida to an altitude of 3,600 miles above the Earth's surface. The two-orbit, four-hour flight test will help engineers evaluate the systems critical to crew safety including the heat shield, parachute system and launch abort system. For more information, visit http://www.nasa.gov/orion. Photo credit: NASA/Glenn Benson
2014-06-06
CAPE CANAVERAL, Fla. -- Inside the Operations and Checkout Building high bay at NASA's Kennedy Space Center in Florida, a Lockheed Martin technician monitors the progress as a crane lowers the Orion service module into the Final Assembly and System Testing, or FAST, cell further down the aisle. The Orion crew module will be stacked on the service module in the FAST cell and then both modules will be put through their final system tests for Exploration Flight Test-1, or EFT-1, before rolling out of the facility for integration with the United Launch Alliance Delta IV Heavy rocket. Orion is the exploration spacecraft designed to carry astronauts to destinations not yet explored by humans, including an asteroid and Mars. It will have emergency abort capability, sustain the crew during space travel and provide safe re-entry from deep space return velocities. The first unpiloted test flight of Orion, EFT-1, is scheduled to launch later this year atop a Delta IV rocket from Cape Canaveral Air Force Station in Florida to an altitude of 3,600 miles above the Earth's surface. The two-orbit, four-hour flight test will help engineers evaluate the systems critical to crew safety including the heat shield, parachute system and launch abort system. For more information, visit http://www.nasa.gov/orion. Photo credit: NASA/Glenn Benson
2014-06-06
CAPE CANAVERAL, Fla. -- Inside the Operations and Checkout Building high bay at NASA's Kennedy Space Center in Florida, NASA and Lockheed Martin engineers and technicians help guide the Orion service module into the Final Assembly and System Testing, or FAST, cell. The Orion crew module will be stacked on the service module in the FAST cell and then both modules will be put through their final system tests for Exploration Flight Test-1, or EFT-1, before rolling out of the facility for integration with the United Launch Alliance Delta IV Heavy rocket. Orion is the exploration spacecraft designed to carry astronauts to destinations not yet explored by humans, including an asteroid and Mars. It will have emergency abort capability, sustain the crew during space travel and provide safe re-entry from deep space return velocities. The first unpiloted test flight of Orion, EFT-1, is scheduled to launch later this year atop a Delta IV rocket from Cape Canaveral Air Force Station in Florida to an altitude of 3,600 miles above the Earth's surface. The two-orbit, four-hour flight test will help engineers evaluate the systems critical to crew safety including the heat shield, parachute system and launch abort system. For more information, visit http://www.nasa.gov/orion. Photo credit: NASA/Glenn Benson
2014-06-06
CAPE CANAVERAL, Fla. -- Inside the Operations and Checkout Building high bay at NASA's Kennedy Space Center in Florida, NASA and Lockheed Martin engineers and technicians monitor the progress as a crane lowers the Orion service module into the Final Assembly and System Testing, or FAST, cell. The Orion crew module will be stacked on the service module in the FAST cell and then both modules will be put through their final system tests for Exploration Flight Test-1, or EFT-1, before rolling out of the facility for integration with the United Launch Alliance Delta IV Heavy rocket. Orion is the exploration spacecraft designed to carry astronauts to destinations not yet explored by humans, including an asteroid and Mars. It will have emergency abort capability, sustain the crew during space travel and provide safe re-entry from deep space return velocities. The first unpiloted test flight of Orion, EFT-1, is scheduled to launch later this year atop a Delta IV rocket from Cape Canaveral Air Force Station in Florida to an altitude of 3,600 miles above the Earth's surface. The two-orbit, four-hour flight test will help engineers evaluate the systems critical to crew safety including the heat shield, parachute system and launch abort system. For more information, visit http://www.nasa.gov/orion. Photo credit: NASA/Glenn Benson
NASA Astrophysics Data System (ADS)
Rusijono; Khotimah, K.
2018-01-01
The purpose of this research was to investigate the effect of implementing the assessment model based on character building to improve discipline and student’s achievement. Assessment model based on character building includes three components, which are the behaviour of students, the efforts, and student’s achievement. This assessment model based on the character building is implemented in science philosophy and educational assessment courses, in Graduate Program of Educational Technology Department, Educational Faculty, Universitas Negeri Surabaya. This research used control group pre-test and post-test design. Data collection method used in this research were observation and test. The observation was used to collect the data about the disciplines of the student in the instructional process, while the test was used to collect the data about student’s achievement. Moreover, the study applied t-test to the analysis of data. The result of this research showed that assessment model based on character building improved discipline and student’s achievement.
2003-09-18
With a laser beam centered on its panel of photovoltaic cells, a lightweight model plane makes the first flight of an aircraft powered by a laser beam inside a building at NASA Marshall Space Flight Center.
111. ARAI Hot cell (ARA626) Building elevations of north, south, ...
111. ARA-I Hot cell (ARA-626) Building elevations of north, south, east, and west sides. Includes details of personnel decontamination area, dark room, and other features. Norman Engineering Company: 961-area/SF-626-A-3. Date: January 1959. Ineel index code no. 068-0626-00-613-102723. - Idaho National Engineering Laboratory, Army Reactors Experimental Area, Scoville, Butte County, ID
Building Leaves and an Understanding of Photosynthesis
ERIC Educational Resources Information Center
Littlejohn, Patty
2007-01-01
Where does cellular respiration take place? How does a plant make food and in turn use the food to produce its own energy? Do animals carry on this process also? To help students answer these and other questions, have them build a model leaf, plant cell, and animal cell. This hands-on project allows students to see and manipulate the reactants and…
PROCESS WATER BUILDING, TRA605. CONTEXTUAL VIEW, CAMERA FACING SOUTHEAST. PROCESS ...
PROCESS WATER BUILDING, TRA-605. CONTEXTUAL VIEW, CAMERA FACING SOUTHEAST. PROCESS WATER BUILDING AND ETR STACK ARE IN LEFT HALF OF VIEW. TRA-666 IS NEAR CENTER, ABUTTED BY SECURITY BUILDING; TRA-626, AT RIGHT EDGE OF VIEW BEHIND BUS. INL NEGATIVE NO. HD46-34-1. Mike Crane, Photographer, 4/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
Billiet, Thomas; Gevaert, Elien; De Schryver, Thomas; Cornelissen, Maria; Dubruel, Peter
2014-01-01
In the present study, we report on the combined efforts of material chemistry, engineering and biology as a systemic approach for the fabrication of high viability 3D printed macroporous gelatin methacrylamide constructs. First, we propose the use and optimization of VA-086 as a photo-initiator with enhanced biocompatibility compared to the conventional Irgacure 2959. Second, a parametric study on the printing of gelatins was performed in order to characterize and compare construct architectures. Hereby, the influence of the hydrogel building block concentration, the printing temperature, the printing pressure, the printing speed, and the cell density were analyzed in depth. As a result, scaffolds could be designed having a 100% interconnected pore network in the gelatin concentration range of 10-20 w/v%. In the last part, the fabrication of cell-laden scaffolds was studied, whereby the application for tissue engineering was tested by encapsulation of the hepatocarcinoma cell line (HepG2). Printing pressure and needle shape was revealed to impact the overall cell viability. Mechanically stable cell-laden gelatin methacrylamide scaffolds with high cell viability (>97%) could be printed. Copyright © 2013 Elsevier Ltd. All rights reserved.
Lv, Decheng
2012-01-01
Numerous researches demonstrated the possibility of derivation of Schwann-like (SC-like) cells in vitro from bone marrow stromal cells (BMSCs). However, the concentration of the induce factors were different in those studies, especially for the critical factors forskolin (FSK) and β-heregulin (HRG). Here, we used a new and useful method to build an integrated microfluidic chip for rapid analyses of the optimal combination between the induce factors FSK and HRG. The microfluidic device was mainly composed of an upstream concentration gradient generator (CGG) and a downstream cell culture module. Rat BMSCs were cultured in the cell chambers for 11 days at the different concentrations of induce factors generated by CGG. The result of immunofluorescence staining on-chip showed that the group of 4.00 µM FSK and 250.00 ng/ml HRG presented an optimal effect to promote the derivation of SC-like cells. Moreover, the optimal SC-like cells obtained on-chip were further tested using DRG co-culture and ELISA to detect their functional performance. Our findings demonstrate that SC-like cells could be obtained with high efficiency and functional performance in the optimal inducers combination. PMID:22880114
Tian, Xiliang; Wang, Shouyu; Zhang, Zhen; Lv, Decheng
2012-01-01
Numerous researches demonstrated the possibility of derivation of Schwann-like (SC-like) cells in vitro from bone marrow stromal cells (BMSCs). However, the concentration of the induce factors were different in those studies, especially for the critical factors forskolin (FSK) and β-heregulin (HRG). Here, we used a new and useful method to build an integrated microfluidic chip for rapid analyses of the optimal combination between the induce factors FSK and HRG. The microfluidic device was mainly composed of an upstream concentration gradient generator (CGG) and a downstream cell culture module. Rat BMSCs were cultured in the cell chambers for 11 days at the different concentrations of induce factors generated by CGG. The result of immunofluorescence staining on-chip showed that the group of 4.00 µM FSK and 250.00 ng/ml HRG presented an optimal effect to promote the derivation of SC-like cells. Moreover, the optimal SC-like cells obtained on-chip were further tested using DRG co-culture and ELISA to detect their functional performance. Our findings demonstrate that SC-like cells could be obtained with high efficiency and functional performance in the optimal inducers combination.
NASA Astrophysics Data System (ADS)
Wang, Qian; Pongkwan, Sitasuwan; Lee, L.; Li, Kai; Nguyen, Huong
2014-05-01
Viral nanoparticles have uniform and well-defined nano-structures and can be produced in large quantities. Several plant viral nanoparticles have been tested in biomedical applications due to the lack of mammalian cell infectivity. We are particularly interested in using Tobacco mosaic virus (TMV), which has been demonstrated to enhance bone tissue regeneration, as a tuneable nanoscale building block for biomaterials development. Unmodified TMV particles have been shown to accelerate osteogenic differentiation of adult stem cells by synergistically upregulating BMP2 and IBSP expression with dexamethasone. However, the lack of affinity to mammalian cell surface resulted in low initial cell adhesion. In this study, to increase cell binding capacity of TMV based material the chemical functionalization of TMV with arginine-glycine-aspartic acid (RGD) peptide was explored. An azide-derivatized RGD peptide was “clicked” to tyrosine residues on TMV outer surface via an efficient copper(I) catalysed azide-alkyne cycloaddition reaction. The ligand spacing is calculated to be 2-4 nm, which could offer a polyvalent ligand clustering effect for enhanced cell receptor signalling, further promoting the proliferation and osteogenic differentiation of bone marrow derived mesenchymal stem cells.
ETR BUILDING, TRA642, INTERIOR. CONSOLE FLOOR, SOUTH HALF. CABLE TUNNEL. ...
ETR BUILDING, TRA-642, INTERIOR. CONSOLE FLOOR, SOUTH HALF. CABLE TUNNEL. CAMERA FACING SOUTH INTO ETR ELECTRICAL BUILDING (TRA-648). INL NEGATIVE NO. HD46-20-2. Mike Crane, Photographer, 2/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
PROCESS WATER BUILDING, TRA605. ONE OF THREE EVAPORATORS BEFORE IT ...
PROCESS WATER BUILDING, TRA-605. ONE OF THREE EVAPORATORS BEFORE IT IS INSTALLED IN UPPER LEVEL OF EAST HALF OF BUILDING. INL NEGATIVE NO. 1533. Unknown Photographer, 3/1/1951 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
SIDE VIEW OF CONTROL BUILDING, CONNECTING TUNNELS VISIBLE AT FAR ...
SIDE VIEW OF CONTROL BUILDING, CONNECTING TUNNELS VISIBLE AT FAR RIGHT, CAPTIVE TEST STAND-2 VISIBLE IN THE DISTANCE AT FAR LEFT, VIEW TOWARDS SOUTHWEST - Glenn L. Martin Company, Titan Missile Test Facilities, Control Building A, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
Encircling the dark: constraining dark energy via cosmic density in spheres
NASA Astrophysics Data System (ADS)
Codis, S.; Pichon, C.; Bernardeau, F.; Uhlemann, C.; Prunet, S.
2016-08-01
The recently published analytic probability density function for the mildly non-linear cosmic density field within spherical cells is used to build a simple but accurate maximum likelihood estimate for the redshift evolution of the variance of the density, which, as expected, is shown to have smaller relative error than the sample variance. This estimator provides a competitive probe for the equation of state of dark energy, reaching a few per cent accuracy on wp and wa for a Euclid-like survey. The corresponding likelihood function can take into account the configuration of the cells via their relative separations. A code to compute one-cell-density probability density functions for arbitrary initial power spectrum, top-hat smoothing and various spherical-collapse dynamics is made available online, so as to provide straightforward means of testing the effect of alternative dark energy models and initial power spectra on the low-redshift matter distribution.
5. BUILDING 8768, SOUTH SIDE AND EAST REAR. TEST STAND ...
5. BUILDING 8768, SOUTH SIDE AND EAST REAR. TEST STAND 1A AT LEFT. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Observation Bunkers for Test Stand 1-A, Test Area 1-120, north end of Jupiter Boulevard, Boron, Kern County, CA
A case study on the structural assessment of fire damaged building
NASA Astrophysics Data System (ADS)
Osman, M. H.; Sarbini, N. N.; Ibrahim, I. S.; Ma, C. K.; Ismail, M.; Mohd, M. F.
2017-11-01
This paper presents a case study on the structural assessment of building damaged by fire and discussed on the site investigations and test results prior to determine the existing condition of the building. The building was on fire for about one hour before it was extinguished. In order to ascertain the integrity of the building, a visual inspection was conducted for all elements (truss, beam, column and wall), followed by non-destructive, load and material tests. The load test was conducted to determine the ability of truss to resist service load, while the material test to determine the residual strength of the material. At the end of the investigation, a structural analysis was carried out to determine the new factor of safety by considering the residual strength. The highlighted was on the truss element due to steel behaviour that is hardly been predicted. Meanwhile, reinforced concrete elements (beam, column and wall) were found externally affected and caused its strength to be considered as sufficient for further used of building. The new factor of safety is equal to 2, considered as the minimum calculated value for the truss member. Therefore, this fire damaged building was found safe and can be used for further application.
Advances in Large Grain/Single Crystal SC Resonators at DESY
DOE Office of Scientific and Technical Information (OSTI.GOV)
W. Singer; A. Brinkmann; A. Ermakov
The main aim of the DESY large grain R&D program is to check whether this option is reasonable to apply for fabrication of ca. 1'000 XFEL cavities. Two aspects are being pursued. On one hand the basic material investigation, on the other hand the material availability, fabrication and preparation procedure. Several single cell large grain cavities of TESLA shape have been fabricated and tested. The best accelerating gradients of 41 MV/m was measured on electropolished cavity. First large grain nine-cell cavities worldwide have been produced under contract of DESY with ACCEL Instruments Co. All three cavities fulfil the XFEL specificationmore » already in first RF test after only BCP (Buffered Chemical Polishing) treatment and 800 degrees C annealing. Accelerating gradient of 27 - 29 MV/m was reached. A fabrication method of single crystal cavity of ILC like shape was proposed. A single cell single crystal cavity was build at the company ACCEL. Accelerating gradient of 37.5 MV/m reached after only 112 microns BCP and in situ baking 120 degrees C for 6 hrs with the quality factor higher as 2x1010. The developed method can be extended on fabrication of multi cell single crystal cavities.« less
Background: Chemical toxicity testing is being transformed by advances in biology and computer modeling, concerns over animal use and the thousands of environmental chemicals lacking toxicity data. EPA's ToxCast program aims to address these concerns by screening and prioritizing chemicals for potential human toxicity using in vitro assays and in silico approaches. Objectives: This project aims to evaluate the use of in vitro assays for understanding the types of molecular and pathway perturbations caused by environmental chemicals and to build initial prioritization models of in vivo toxicity. Methods: We tested 309 mostly pesticide active chemicals in 467 assays across 9 technologies, including high-throughput cell-free assays and cell-based assays in multiple human primary cells and cell lines, plus rat primary hepatocytes. Both individual and composite scores for effects on genes and pathways were analyzed. Results: Chemicals display a broad spectrum of activity at the molecular and pathway levels. Many expected interactions are seen, including endocrine and xenobiotic metabolism enzyme activity. Chemicals range in promiscuity across pathways, from no activity to affecting dozens of pathways. We find a statistically significant inverse association between the number of pathways perturbed by a chemical at low in vitro concentrations and the lowest in vivo dose at which a chemical causes toxicity. We also find associations between a small set in vitro ass
A combinatorial code for pattern formation in Drosophila oogenesis.
Yakoby, Nir; Bristow, Christopher A; Gong, Danielle; Schafer, Xenia; Lembong, Jessica; Zartman, Jeremiah J; Halfon, Marc S; Schüpbach, Trudi; Shvartsman, Stanislav Y
2008-11-01
Two-dimensional patterning of the follicular epithelium in Drosophila oogenesis is required for the formation of three-dimensional eggshell structures. Our analysis of a large number of published gene expression patterns in the follicle cells suggests that they follow a simple combinatorial code based on six spatial building blocks and the operations of union, difference, intersection, and addition. The building blocks are related to the distribution of inductive signals, provided by the highly conserved epidermal growth factor receptor and bone morphogenetic protein signaling pathways. We demonstrate the validity of the code by testing it against a set of patterns obtained in a large-scale transcriptional profiling experiment. Using the proposed code, we distinguish 36 distinct patterns for 81 genes expressed in the follicular epithelium and characterize their joint dynamics over four stages of oogenesis. The proposed combinatorial framework allows systematic analysis of the diversity and dynamics of two-dimensional transcriptional patterns and guides future studies of gene regulation.
Single Day Construction of Multigene Circuits with 3G Assembly.
Halleran, Andrew D; Swaminathan, Anandh; Murray, Richard M
2018-05-18
The ability to rapidly design, build, and test prototypes is of key importance to every engineering discipline. DNA assembly often serves as a rate limiting step of the prototyping cycle for synthetic biology. Recently developed DNA assembly methods such as isothermal assembly and type IIS restriction enzyme systems take different approaches to accelerate DNA construction. We introduce a hybrid method, Golden Gate-Gibson (3G), that takes advantage of modular part libraries introduced by type IIS restriction enzyme systems and isothermal assembly's ability to build large DNA constructs in single pot reactions. Our method is highly efficient and rapid, facilitating construction of entire multigene circuits in a single day. Additionally, 3G allows generation of variant libraries enabling efficient screening of different possible circuit constructions. We characterize the efficiency and accuracy of 3G assembly for various construct sizes, and demonstrate 3G by characterizing variants of an inducible cell-lysis circuit.
A Living Laboratory for Building-Grid Integration
Shankle, Steve; Goyal, Siddharth
2018-01-16
At PNNL weâre developing a test bed for control of how buildings interact with the gridâan important step toward helping buildings achieve their potential for reducing energy use and improving the management of the nationâs power systems. The test bed works by allowing researchers to conduct experiments on PNNLâs specially-equipped Systems Engineering Building. This unique resource will help the Department of Energy achieve its mission of reducing buildings energy use by 50 percent by 2030.
Grabowska, Wioleta; Suszek, Małgorzata; Wnuk, Maciej; Lewinska, Anna; Wasiak, Emilia; Sikora, Ewa; Bielak-Zmijewska, Anna
2016-01-01
It is believed that curcumin, a component of the turmeric that belongs to hormetins, possesses anti-aging propensity. This property of curcumin can be partially explained by its influence on the level of sirtuins. Previously, we have shown that relatively high (2.5-10 μM) doses of curcumin induce senescence of cancer cells and cells building the vasculature. In the present study we examined whether curcumin at low doses (0.1 and 1 μM) is able to delay cell senescence and upregulate the level of sirtuins in human cells building the vasculature, namely vascular smooth muscle (VSMC) and endothelial (EC) cells. To this end we used cells senescing in a replicative and premature manner. We showed that low doses of curcumin in case of VSMC neither postponed the replicative senescence nor protected from premature senescence induced by doxorubicin. Moreover, curcumin slightly accelerated replicative senescence of EC. Despite some fluctuations, a clear increasing tendency in the level of sirtuins was observed in curcumin-treated young, senescing or already senescent cells. Sirtuin activation could be caused by the activation of AMPK resulting from superoxide elevation and ATP reduction. Our results show that curcumin at low doses can increase the level of sirtuins without delaying senescence of VSMC. PMID:27034011
Unsupervised building detection from irregularly spaced LiDAR and aerial imagery
NASA Astrophysics Data System (ADS)
Shorter, Nicholas Sven
As more data sources containing 3-D information are becoming available, an increased interest in 3-D imaging has emerged. Among these is the 3-D reconstruction of buildings and other man-made structures. A necessary preprocessing step is the detection and isolation of individual buildings that subsequently can be reconstructed in 3-D using various methodologies. Applications for both building detection and reconstruction have commercial use for urban planning, network planning for mobile communication (cell phone tower placement), spatial analysis of air pollution and noise nuisances, microclimate investigations, geographical information systems, security services and change detection from areas affected by natural disasters. Building detection and reconstruction are also used in the military for automatic target recognition and in entertainment for virtual tourism. Previously proposed building detection and reconstruction algorithms solely utilized aerial imagery. With the advent of Light Detection and Ranging (LiDAR) systems providing elevation data, current algorithms explore using captured LiDAR data as an additional feasible source of information. Additional sources of information can lead to automating techniques (alleviating their need for manual user intervention) as well as increasing their capabilities and accuracy. Several building detection approaches surveyed in the open literature have fundamental weaknesses that hinder their use; such as requiring multiple data sets from different sensors, mandating certain operations to be carried out manually, and limited functionality to only being able to detect certain types of buildings. In this work, a building detection system is proposed and implemented which strives to overcome the limitations seen in existing techniques. The developed framework is flexible in that it can perform building detection from just LiDAR data (first or last return), or just nadir, color aerial imagery. If data from both LiDAR and aerial imagery are available, then the algorithm will use them both for improved accuracy. Additionally, the proposed approach does not employ severely limiting assumptions thus enabling the end user to apply the approach to a wider variety of different building types. The proposed approach is extensively tested using real data sets and it is also compared with other existing techniques. Experimental results are presented.
1987-08-01
01 0 4 In w el4 ’ n r fl f4 r, V 0 1" * * * - ~ m ~c- I0 1-0- Q~ 3c 3c~ AdI-. * tp~dip Eu - 4j :: .( - * -U I’ -L - UJ a. Zw a Inc., ɘ L.3. 2-3...swale ap- proximately 100 feet south of Building 222 (Figure 3-7), the Jet Engine Test cell . With the exception of VOC, all param- eters detected at 15-B
Loss and gain of cone types in vertebrate ciliary photoreceptor evolution.
Musser, Jacob M; Arendt, Detlev
2017-11-01
Ciliary photoreceptors are a diverse cell type family that comprises the rods and cones of the retina and other related cell types such as pineal photoreceptors. Ciliary photoreceptor evolution has been dynamic during vertebrate evolution with numerous gains and losses of opsin and phototransduction genes, and changes in their expression. For example, early mammals lost all but two cone opsins, indicating loss of cone receptor types in response to nocturnal lifestyle. Our review focuses on the comparison of specifying transcription factors and cell type-specific transcriptome data in vertebrate retinae to build and test hypotheses on ciliary photoreceptor evolution. Regarding cones, recent data reveal that a combination of factors specific for long-wavelength sensitive opsin (Lws)- cones in non-mammalian vertebrates (Thrb and Rxrg) is found across all differentiating cone photoreceptors in mice. This suggests that mammalian ancestors lost all but one ancestral cone type, the Lws-cone. We test this hypothesis by a correlation analysis of cone transcriptomes in mouse and chick, and find that, indeed, transcriptomes of all mouse cones are most highly correlated to avian Lws-cones. These findings underscore the importance of specifying transcription factors in tracking cell type evolution, and shed new light on the mechanisms of cell type loss and gain in retina evolution. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.
LPT. Shield test facility test building interior (TAN646). Camera facing ...
LPT. Shield test facility test building interior (TAN-646). Camera facing south. Distant pool contained EBOR reactor; near pool was intended for fuel rod storage. Other post-1970 activity equipment remains in pool. INEEL negative no. HD-40-9-4 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID
LPT. Shield test facility test building interior (TAN646). Camera points ...
LPT. Shield test facility test building interior (TAN-646). Camera points down into interior of north pool. Equipment on wall is electronical bus used for post-1970 experiment. Personnel ladder at right. INEEL negative no. HD-40-9-1 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID
3. BUILDING 8767, NORTH REAR AND WEST SIDE, TEST STAND ...
3. BUILDING 8767, NORTH REAR AND WEST SIDE, TEST STAND 1-A AT FAR RIGHT. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Observation Bunkers for Test Stand 1-A, Test Area 1-120, north end of Jupiter Boulevard, Boron, Kern County, CA
MTR BUILDING, TRA603. SOUTHEAST CORNER, EAST SIDE FACING TOWARD RIGHT ...
MTR BUILDING, TRA-603. SOUTHEAST CORNER, EAST SIDE FACING TOWARD RIGHT OF VIEW. CAMERA FACING NORTHWEST. LIGHT-COLORED PROJECTION AT LEFT IS ENGINEERING SERVICES BUILDING, TRA-635. SMALL CONCRETE BLOCK BUILDING AT CENTER OF VIEW IS FAST CHOPPER DETECTOR HOUSE, TRA-665. INL NEGATIVE NO. HD46-43-3. Mike Crane, Photographer, 4/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
NASA Technical Reports Server (NTRS)
Springer, Darlene
1989-01-01
Different aspects of Space Station Environmental Control and Life Support System (ECLSS) testing are currently taking place at Marshall Space Flight Center (MSFC). Unique to this testing is the variety of test areas and the fact that all are located in one building. The north high bay of building 4755, the Core Module Integration Facility (CMIF), contains the following test areas: the Subsystem Test Area, the Comparative Test Area, the Process Material Management System (PMMS), the Core Module Simulator (CMS), the End-use Equipment Facility (EEF), and the Pre-development Operational System Test (POST) Area. This paper addresses the facility that supports these test areas and briefly describes the testing in each area. Future plans for the building and Space Station module configurations will also be discussed.
9. BUILDING NO. 620B, FRICTION PENDULUM BUILDING. 29FOOT DROP TOWER ...
9. BUILDING NO. 620-B, FRICTION PENDULUM BUILDING. 29-FOOT DROP TOWER SITS BEHIND BLAST SHIELD IN FRONT OF BUILDING. - Picatinny Arsenal, 600 Area, Test Areas District, State Route 15 near I-80, Dover, Morris County, NJ
ETR AND MTR COMPLEXES IN CONTEXT. CAMERA FACING NORTHERLY. FROM ...
ETR AND MTR COMPLEXES IN CONTEXT. CAMERA FACING NORTHERLY. FROM BOTTOM TO TOP: ETR COOLING TOWER, ELECTRICAL BUILDING AND LOW-BAY SECTION OF ETR BUILDING, HEAT EXCHANGER BUILDING (WITH U SHAPED YARD), COMPRESSOR BUILDING. MTR REACTOR SERVICES BUILDING IS ATTACHED TO SOUTH WALL OF MTR. WING A IS ATTACHED TO BALCONY FLOOR OF MTR. NEAR UPPER RIGHT CORNER OF VIEW IS MTR PROCESS WATER BUILDING. WING B IS AT FAR WEST END OF COMPLEX. NEAR MAIN GATE IS GAMMA FACILITY, WITH "COLD" BUILDINGS BEYOND: RAW WATER STORAGE TANKS, STEAM PLANT, MTR COOLING TOWER PUMP HOUSE AND COOLING TOWER. INL NEGATIVE NO. 56-4101. - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
Initial operation of a solar heating and cooling system in a full-scale solar building test facility
NASA Technical Reports Server (NTRS)
Knoll, R. H.; Miao, D.; Hamlet, I. L.; Jensen, R. N.
1976-01-01
The Solar Building Test Facility (SBTF) was constructed to advance the technology for heating and cooling of office buildings with solar energy. Its purposes are to (1) test system components which include high-performing collectors, (2) test the performance of a complete solar heating and cooling system, (3) investigate component interactions, and (4) investigate durability, maintenance and reliability of components. The SBTF consists of a 50,000 square foot office building modified to accept solar heated water for operation of an absorption air conditioner and for the baseboard heating system. A 12,666 square foot solar collector field with a 30,000 gallon storage tank provides the solar heated water. A description of the system and the collectors selected is printed along with the objectives, test approach, expected system performance, and some preliminary results.
High rate discharge studies of Li/SO2 cells
NASA Astrophysics Data System (ADS)
Dallek, S.; Bis, R. F.; Bowers, F. M.
Experimental D-size spirally-wound 3V 10 A-hr cells were used in the reported study. The cells were instrumented with four iron-constantan thermocouples. It was found that during a discharge of a thermally insulated cell, the greatest amount of heat builds up in the center of the cell. The heat build-up appears to be purely resistive in nature. Concerning the safe use of these cells, it has been demonstrated that at very high rates of discharge, e.g., 10 A, cells may vent violently and cause a fire. At this high rate of discharge, the internal cell temperature exceeded the melting point of the lithium anode which is in the form of an unsupported strip. Thus, contact of the highly reactive molten lithium with other reactive species in the cell is possible under these conditions and could result in a very exothermic chemical reaction.
115. ARAI Details of hot cell section of building ARA626. ...
115. ARA-I Details of hot cell section of building ARA-626. Shows location of high density concrete, viewing windows, filters, monorail crane, bridge crane, and other details. Norman Engineering Company 961-area/SF-626-MS-1. Date: January 1959. Ineel index code no. 068-0626-40-613-102737. - Idaho National Engineering Laboratory, Army Reactors Experimental Area, Scoville, Butte County, ID
ETR BUILDING, TRA642, INTERIOR. FIRST FLOOR. INSIDE UTILITY CORRIDOR ALONG ...
ETR BUILDING, TRA-642, INTERIOR. FIRST FLOOR. INSIDE UTILITY CORRIDOR ALONG SOUTH PERIMETER WALL (COMMON TO ELECTRICAL BUILDING, TRA-648). CAMERA FACES WEST. INL NEGATIVE NO. HD46-16-2. Mike Crane, Photographer, 2/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
ETR, TRA642. ELEVATIONS. METAL SIDING. OFFICE BUILDING (TRA647) AND ELECTRICAL ...
ETR, TRA-642. ELEVATIONS. METAL SIDING. OFFICE BUILDING (TRA-647) AND ELECTRICAL BUILDING (TRA-648) ATTACHED. KAISER ETR-5528-MTR-642-A-11, 11/1955. INL INDEX NO. 532-0642-00-486-100919, REV. 3. - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
9. Credit JPL. Photographic copy of drawing, engineering drawing showing ...
9. Credit JPL. Photographic copy of drawing, engineering drawing showing structure of Test Stand 'A' (Building 4202/E-3) and its relationship to the Monitor Building or blockhouse (Building 4203/E-4) when a reinforced concrete machinery room was added to the west side of Test Stand 'A' in 1955. California Institute of Technology, Jet Propulsion Laboratory, Plant Engineering 'Electrical Layout - Muroc, Test Stand & Refrigeration Equipment Room,' drawing no. E3/7-0, April 6, 1955. - Jet Propulsion Laboratory Edwards Facility, Test Stand A, Edwards Air Force Base, Boron, Kern County, CA
Design of stationary PEFC system configurations to meet heat and power demands
NASA Astrophysics Data System (ADS)
Wallmark, Cecilia; Alvfors, Per
This paper presents heat and power efficiencies of a modeled PEFC system and the methods used to create the system configuration. The paper also includes an example of a simulated fuel cell system supplying a building in Sweden with heat and power. The main method used to create an applicable fuel cell system configuration is pinch technology. This technology is used to evaluate and design a heat exchanger network for a PEFC system working under stationary conditions, in order to find a solution with high heat utilization. The heat exchanger network in the system connecting the reformer, the burner, gas cleaning, hot-water storage and the PEFC stack will affect the heat transferred to the hot-water storage and thereby the heating of the building. The fuel, natural gas, is reformed to a hydrogen-rich gas within a slightly pressurized system. The fuel processor investigated is steam reforming, followed by high- and low-temperature shift reactors and preferential oxidation. The system is connected to the electrical grid for backup and peak demands and to a hot-water storage to meet the varying heat demand for the building. The procedure for designing the fuel cell system installation as co-generation system is described, and the system is simulated for a specific building in Sweden during 1 year. The results show that the fuel cell system in combination with a burner and hot-water storage could supply the building with the required heat without exceeding any of the given limitations. The designed co-generation system will provide the building with most of its power requirements and would further generate income by sale of electricity to the power grid.
A novel anti-CD22 scFv-apoptin fusion protein induces apoptosis in malignant B-cells.
Agha Amiri, Solmaz; Shahhosseini, Soraya; Zarei, Najmeh; Khorasanizadeh, Dorsa; Aminollahi, Elahe; Rezaie, Faegheh; Zargari, Mehryar; Azizi, Mohammad; Khalaj, Vahid
2017-12-01
CD22 marker is a highly internalizing antigen which is located on the surface of B-cells and is being used as a promising target for treatment of B cell malignancies. Monoclonal antibodies targeting CD22 have been introduced and some are currently under investigation in clinical trials. Building on the success of antibody drug conjugates, we developed a fusion protein consisting of a novel anti-CD22 scFv and apoptin and tested binding and therapeutic effects in lymphoma cells. The recombinant protein was expressed in E. coli and successfully purified and refolded. In vitro binding analysis by immunofluorescence and flow cytometry demonstrated that the recombinant protein specifically binds to CD22 positive Raji cells but not to CD22 negative Jurkat cells. The cytotoxic properties of scFv-apoptin were assessed by an MTT assay and Annexin V/PI flow cytometry analysis and showed that the recombinant protein induced apoptosis preferentially in Raji cells with no detectable effects in Jurkat cells. Our findings indicated that the recombinant anti-CD22 scFv-apoptin fusion protein could successfully cross the cell membrane and induce apoptosis with high specificity, make it as a promising molecule for immunotherapy of B-cell malignancies.
Surface-specific additive manufacturing test artefacts
NASA Astrophysics Data System (ADS)
Townsend, Andrew; Racasan, Radu; Blunt, Liam
2018-06-01
Many test artefact designs have been proposed for use with additive manufacturing (AM) systems. These test artefacts have primarily been designed for the evaluation of AM form and dimensional performance. A series of surface-specific measurement test artefacts designed for use in the verification of AM manufacturing processes are proposed here. Surface-specific test artefacts can be made more compact because they do not require the large dimensions needed for accurate dimensional and form measurements. The series of three test artefacts are designed to provide comprehensive information pertaining to the manufactured surface. Measurement possibilities include deviation analysis, surface texture parameter data generation, sub-surface analysis, layer step analysis and build resolution comparison. The test artefacts are designed to provide easy access for measurement using conventional surface measurement techniques, for example, focus variation microscopy, stylus profilometry, confocal microscopy and scanning electron microscopy. Additionally, the test artefacts may be simply visually inspected as a comparative tool, giving a fast indication of process variation between builds. The three test artefacts are small enough to be included in every build and include built-in manufacturing traceability information, making them a convenient physical record of the build.
2014-06-06
CAPE CANAVERAL, Fla. -- Inside the Operations and Checkout Building high bay at NASA's Kennedy Space Center in Florida, a Lockheed Martin technician monitors the progress as a crane is used to lift the Orion service module from a test stand and move it to the Final Assembly and System Testing, or FAST, cell further down the aisle. The Orion crew module will be stacked on the service module in the FAST cell and then both modules will be put through their final system tests for Exploration Flight Test-1, or EFT-1, prior to rolling out of the facility for integration with the United Launch Alliance Delta IV Heavy rocket. Orion is the exploration spacecraft designed to carry astronauts to destinations not yet explored by humans, including an asteroid and Mars. It will have emergency abort capability, sustain the crew during space travel and provide safe re-entry from deep space return velocities. The first unpiloted test flight of Orion, EFT-1, is scheduled to launch later this year atop a Delta IV rocket from Cape Canaveral Air Force Station in Florida to an altitude of 3,600 miles above the Earth's surface. The two-orbit, four-hour flight test will help engineers evaluate the systems critical to crew safety including the heat shield, parachute system and launch abort system. For more information, visit http://www.nasa.gov/orion. Photo credit: NASA/Glenn Benson
2014-06-06
CAPE CANAVERAL, Fla. -- Inside the Operations and Checkout Building high bay at NASA's Kennedy Space Center in Florida, NASA and Lockheed Martin technicians and engineers monitor the progress as a crane is used to lift the Orion service module from a test stand and move it to the Final Assembly and System Testing, or FAST, cell further down the aisle. The Orion crew module will be stacked on the service module in the FAST cell and then both modules will be put through their final system tests for Exploration Flight Test-1, or EFT-1, before rolling out of the facility for integration with the United Launch Alliance Delta IV Heavy rocket. Orion is the exploration spacecraft designed to carry astronauts to destinations not yet explored by humans, including an asteroid and Mars. It will have emergency abort capability, sustain the crew during space travel and provide safe re-entry from deep space return velocities. The first unpiloted test flight of Orion, EFT-1, is scheduled to launch later this year atop a Delta IV rocket from Cape Canaveral Air Force Station in Florida to an altitude of 3,600 miles above the Earth's surface. The two-orbit, four-hour flight test will help engineers evaluate the systems critical to crew safety including the heat shield, parachute system and launch abort system. For more information, visit http://www.nasa.gov/orion. Photo credit: NASA/Glenn Benson
NASA Astrophysics Data System (ADS)
Sun, J.; Jasieniak, J. J.
2017-03-01
Semi-transparent solar cells are a type of technology that combines the benefits of visible light transparency and light-to-electricity conversion. One of the biggest opportunities for such technologies is in their integration as windows and skylights within energy-sustainable buildings. Currently, such building integrated photovoltaics (BIPV) are dominated by crystalline silicon based modules; however, the opaque nature of silicon creates a unique opportunity for the adoption of emerging photovoltaic candidates that can be made truly semi-transparent. These include: amorphous silicon-, kesterite-, chalcopyrite-, CdTe-, dye-sensitized-, organic- and perovskite- based systems. For the most part, amorphous silicon has been the workhorse in the semi-transparent solar cell field owing to its established, low-temperature fabrication processes. Excitement around alternative classes, particularly perovskites and the inorganic candidates, has recently arisen because of the major efficiency gains exhibited by these technologies. Importantly, each of these presents unique opportunities and challenges within the context of BIPV. This topic review provides an overview into the broader benefits of semi-transparent solar cells as building-integrated features, as well as providing the current development status into all of the major types of semi-transparent solar cells technologies.
DOE Office of Scientific and Technical Information (OSTI.GOV)
T. M. Fitzmaurice
2001-04-01
The purpose of this Closure Report (CR) is to provide documentation of the completed corrective action at the Test Cell A Leachfield System and to provide data confirming the corrective action. The Test Cell A Leachfield System is identified in the Federal Facility Agreement and Consent Order (FFACO) of 1996 as Corrective Action Unit (CAU) 261. Remediation of CAU 261 is required under the FFACO (1996). CAU 261 is located in Area 25 of the Nevada Test Site (NTS) which is approximately 140 kilometers (87 miles) northwest of Las Vegas, Nevada (Figure 1). CAU 261 consists of two Corrective Actionmore » Sites (CASS): CAS 25-05-01, Leachfield; and CAS 25-05-07, Acid Waste Leach Pit (AWLP) (Figures 2 and 3). Test Cell A was operated during the 1960s and 1970s to support the Nuclear Rocket Development Station. Various operations within Building 3124 at Test Cell A resulted in liquid waste releases to the Leachfield and the AWLP. The following existing site conditions were reported in the Corrective Action Decision Document (CADD) (U.S. Department of Energy, Nevada Operations Office [DOE/NV], 1999): Soil in the leachfield was found to exceed the Nevada Division of Environmental Protection (NDEP) Action Level for petroleum hydrocarbons, the U.S. Environmental Protection Agency (EPA) preliminary remediation goals for semi volatile organic compounds, and background concentrations for strontium-90; Soil below the sewer pipe and approximately 4.5 meters (m) (15 feet [ft]) downstream of the initial outfall was found to exceed background concentrations for cesium-137 and strontium-90; Sludge in the leachfield septic tank was found to exceed the NDEP Action Level for petroleum hydrocarbons and to contain americium-241, cesium-137, uranium-234, uranium-238, potassium-40, and strontium-90; No constituents of concern (COC) were identified at the AWLP. The NDEP-approved CADD (DOWNV, 1999) recommended Corrective Action Alternative 2, ''Closure of the Septic Tank and Distribution Box, Partial Excavation, and Administrative Controls.'' The corrective action was performed following the NDEP-approved Corrective Action Plan (CAP) (DOE/NV, 2000).« less
The methodology of choice Cam-Clay model parameters for loess subsoil
NASA Astrophysics Data System (ADS)
Nepelski, Krzysztof; Błazik-Borowa, Ewa
2018-01-01
The paper deals with the calibration method of FEM subsoil model described by the constitutive Cam-Clay model. The four-storey residential building and solid substrate are modelled. Identification of the substrate is made using research drilling, CPT static tests, DMT Marchetti dilatometer, and laboratory tests. Latter are performed on the intact soil specimens which are taken from the wide planning trench at the depth of foundation. The real building settlements was measured as the vertical displacement of benchmarks. These measurements were carried out periodically during the erection of the building and its operation. Initially, the Cam Clay model parameters were determined on the basis of the laboratory tests, and later, they were corrected by taking into consideration numerical analyses results (whole building and its parts) and real building settlements.
SPERTI Terminal Building (PER604) is under construction in foreground, with ...
SPERT-I Terminal Building (PER-604) is under construction in foreground, with vertical metal siding partially affixed to gable end of building. Utility lines are laid in shallow trench to Reactor Pit and Instrument Cell Buildings also under construction in distance. Photographer: R.G. Larsen. Date: April 22, 1955. INEEL negative no. 55-1001 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID
FAST CHOPPER BUILDING, TRA665. CAMERA FACING NORTH. NOTE BRICKEDIN WINDOW ...
FAST CHOPPER BUILDING, TRA-665. CAMERA FACING NORTH. NOTE BRICKED-IN WINDOW ON RIGHT SIDE (BELOW PAINTED NUMERALS "665"). SLIDING METAL DOOR ON COVERED RAIL AT UPPER LEVEL. SHELTERED ENTRANCE TO STEEL SHIELDING DOOR. DOOR INTO MTR SERVICE BUILDING, TRA-635, STANDS OPEN. MTR BEHIND CHOPPER BUILDING. INL NEGATIVE NO. HD42-1. Mike Crane, Photographer, 3/2004 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery.
Karim, Ashty S; Jewett, Michael C
2016-07-01
Speeding up design-build-test (DBT) cycles is a fundamental challenge facing biochemical engineering. To address this challenge, we report a new cell-free protein synthesis driven metabolic engineering (CFPS-ME) framework for rapid biosynthetic pathway prototyping. In our framework, cell-free cocktails for synthesizing target small molecules are assembled in a mix-and-match fashion from crude cell lysates either containing selectively enriched pathway enzymes from heterologous overexpression or directly producing pathway enzymes in lysates by CFPS. As a model, we apply our approach to n-butanol biosynthesis showing that Escherichia coli lysates support a highly active 17-step CoA-dependent n-butanol pathway in vitro. The elevated degree of flexibility in the cell-free environment allows us to manipulate physiochemical conditions, access enzymatic nodes, discover new enzymes, and prototype enzyme sets with linear DNA templates to study pathway performance. We anticipate that CFPS-ME will facilitate efforts to define, manipulate, and understand metabolic pathways for accelerated DBT cycles without the need to reengineer organisms. Copyright © 2016 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.
NASA Astrophysics Data System (ADS)
Šafka, J.; Ackermann, M.; Voleský, L.
2016-04-01
This paper deals with establishing of building parameters for 1.2344 (H13) tool steel processed using Selective Laser Melting (SLM) technology with layer thickness of 50 µm. In the first part of the work, testing matrix of models in the form of a cube with chamfered edge were built under various building parameters such as laser scanning speed and laser power. Resulting models were subjected to set of tests including measurement of surface roughness, inspection of inner structure with aid of Light Optical Microscopy and Scanning Electron Microscopy and evaluation of micro-hardness. These tests helped us to evaluate an influence of changes in building strategy to the properties of the resulting model. In the second part of the work, mechanical properties of the H13 steel were examined. For this purpose, the set of samples in the form of “dog bone” were printed under three different alignments towards the building plate and tested on universal testing machine. Mechanical testing of the samples should then reveal if the different orientation and thus different layering of the material somehow influence its mechanical properties. For this type of material, the producer provides the parameters for layer thickness of 30 µm only. Thus, our 50 µm building strategy brings shortening of the building time which is valuable especially for large models. Results of mechanical tests show slight variation in mechanical properties for various alignment of the sample.
Kahn Bani Sa’ad Correctional Facility, Kahn Bani Sa’ad, Iraq
2008-07-25
building (Building No. 07/03): 1. Ground floor columns were tested with a Schmidt hammer devise (measures the elastic properties or strength of...3. First floor columns – Schmidt hammer and ultrasonic tests were conducted on all the first floor columns. The test results showed the strength...Building Nos. 07/02 and 07/01): 1. Ground floor columns – Using a Schmidt hammer device, very few columns showed low strength. Further study and
FLOOR PLAN OF MAIN PROCESSING BUILDING (CPP601) BASEMENT SHOWING PROCESS ...
FLOOR PLAN OF MAIN PROCESSING BUILDING (CPP-601) BASEMENT SHOWING PROCESS CORRIDOR AND EIGHTEEN CELLS. TO LEFT IS LABORATORY BUILDING (CPP-602). INL DRAWING NUMBER 200-0601-00-706-051981. ALTERNATE ID NUMBER CPP-E-1981. - Idaho National Engineering Laboratory, Idaho Chemical Processing Plant, Fuel Reprocessing Complex, Scoville, Butte County, ID
Investigations of DC power supplies with optoelectronic transducers and RF energy converters
NASA Astrophysics Data System (ADS)
Guzowski, B.; Gozdur, R.; Bernacki, L.; Lakomski, M.
2016-04-01
Fiber Distribution Cabinets (FDC) monitoring systems are increasingly popular. However it is difficult to realize such system in passive FDC, due to lack of source of power supply. In this paper investigation of four different DC power supplies with optoelectronic transducers is described. Two converters: photovoltaic power converter and PIN photodiode can convert the light transmitted through the optical fiber to electric energy. Solar cell and antenna RF-PCB are also tested. Results presented in this paper clearly demonstrate that it is possible to build monitoring system in passive FDC. During the tests maximum obtained output power was 11 mW. However all converters provided enough power to excite 32-bit microcontroller with ARM-cores and digital thermometer.
Fostering synergy between cell biology and systems biology.
Eddy, James A; Funk, Cory C; Price, Nathan D
2015-08-01
In the shared pursuit of elucidating detailed mechanisms of cell function, systems biology presents a natural complement to ongoing efforts in cell biology. Systems biology aims to characterize biological systems through integrated and quantitative modeling of cellular information. The process of model building and analysis provides value through synthesizing and cataloging information about cells and molecules, predicting mechanisms and identifying generalizable themes, generating hypotheses and guiding experimental design, and highlighting knowledge gaps and refining understanding. In turn, incorporating domain expertise and experimental data is crucial for building towards whole cell models. An iterative cycle of interaction between cell and systems biologists advances the goals of both fields and establishes a framework for mechanistic understanding of the genome-to-phenome relationship. Crown Copyright © 2015. Published by Elsevier Ltd. All rights reserved.
6. Historic American Buildings Survey Nathaniel R. Ewan, Photographer March ...
6. Historic American Buildings Survey Nathaniel R. Ewan, Photographer March 3, 1937 INTERIOR - MURDERER'S CELL - SECOND FLOOR - Burlington County Prison, 128 High Street, Mount Holly, Burlington County, NJ
1. BUILDING 8698, TEST STAND 13, WEST ELEVATION. NOTE TUNNEL ...
1. BUILDING 8698, TEST STAND 1-3, WEST ELEVATION. NOTE TUNNEL BETWEEN BLDG. 8668 AND TEST STAND 1-3. TEST AREA 1-120 IN THE MIDDLE DISTANCE, AND TEST AREA 1-125 ON THE HORIZON. Looking northeast from the roof of Building 8668, Instrumentation and Control Center. Note: Photograph CA-236-F-2 is an 8" x 10" enlargement from a 4" x 5" negative. This view is a photocopy of a recent resin coated print made from a print held at the Main Base History Office, Edwards Air Force Base, California. Photographer unknown. Date and file number unknown. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Test Stand 1-3, Test Area 1-115, northwest end of Saturn Boulevard, Boron, Kern County, CA
X-33/RLV Program Aerospike Engines
NASA Technical Reports Server (NTRS)
1999-01-01
Substantial progress was made during the past year in support of the X-33/RLV program. X-33 activity was directed towards completing the remaining design work and building hardware to support test activities. RLV work focused on the nozzle ramp and powerpack technology tasks and on supporting vehicle configuration studies. On X-33, the design activity was completed to the detail level and the remainder of the drawings were released. Component fabrication and engine assembly activity was initiated, and the first two powerpacks and the GSE and STE needed to support powerpack testing were completed. Components fabrication is on track to support the first engine assembly schedule. Testing activity included powerpack testing and component development tests consisting of thrust cell single cell testing, CWI system spider testing, and EMA valve flow and vibration testing. Work performed for RLV was divided between engine system and technology development tasks. Engine system activity focused on developing the engine system configuration and supporting vehicle configuration studies. Also, engine requirements were developed, and engine performance analyses were conducted. In addition, processes were developed for implementing reliability, mass properties, and cost controls during design. Technology development efforts were divided between powerpack and nozzle ramp technology tasks. Powerpack technology activities were directed towards the development of a prototype powerpack and a ceramic turbine technology demonstrator (CTTD) test article which will allow testing of ceramic turbines and a close-coupled gas generator design. Nozzle technology efforts were focused on the selection of a composite nozzle supplier and on the fabrication and test of composite nozzle coupons.
1. TEST AREA 1115, SOUTH PART OF SUPPORT COMPLEX, LOOKING ...
1. TEST AREA 1-115, SOUTH PART OF SUPPORT COMPLEX, LOOKING TO EAST FROM ABOVE BUILDING 8655, THE FUEL STORAGE TANK FARM, IN FOREGROUND SHADOW. AT THE RIGHT IS BUILDING 8660, ELECTRICAL SUBSTATION; TO ITS LEFT IS BUILDING 8663, THE HELIUM COMPRESSION PLANT. THE LIGHT TONED STRUCTURE IN THE MIDDLE DISTANCE, CENTER, IS THE MACHINE SHOP FOR TEST STAND 1-3. IN THE FAR DISTANCE IS TEST STAND 1-A, WITH THE WHITE SPHERICAL TANKS, AND TEST STAND 2-A TO ITS RIGHT. ALONG THE HORIZON FROM FAR LEFT ARE TEST STAND 1-D, TEST STAND 1-C, WATER TANKS ABOVE TEST AREA 1-125, AND TEST STAND 1-B IN TEST AREA 1-120. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Leuhman Ridge near Highways 58 & 395, Boron, Kern County, CA
View of building 11070, with building 11050 in the background ...
View of building 11070, with building 11050 in the background (left side). Looking northeast. - Naval Ordnance Test Station Inyokern, China Lake Pilot Plant, Maintenance Shop, C Street, China Lake, Kern County, CA
PBF Reactor Building (PER620). PBF crane holds fuel test assembly ...
PBF Reactor Building (PER-620). PBF crane holds fuel test assembly aloft prior to lowering into reactor for test. Date: 1982. INEEL negative no. 82-4909 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID
Building Physics Test Cases | Buildings | NREL
building physics test cases in BESTEST-EX. In these cases, the model inputs that describe the house are programs. This diagram provides an overview of the BESTEST-EX physics case process. On the left side of the diagram is a box labeled "BESTEST-EX Document" with a list that contains two bulleted items. The
ETR BUILDING, TRA642, INTERIOR. BASEMENT. CORRIDOR ALONG WEST WALL OF ...
ETR BUILDING, TRA-642, INTERIOR. BASEMENT. CORRIDOR ALONG WEST WALL OF BUILDING, WHICH IS AT RIGHT OF VIEW. AUDIO ALARM IS ALONG WALL AT RIGHT. CAMERA FACES SOUTH. INL NEGATIVE NO. HD46-30-1. Mike Crane, Photographer, 2/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
2. SOUTHEAST SIDE AND NORTHEAST REAR. SHOP BUILDING IN DISTANCE. ...
2. SOUTHEAST SIDE AND NORTHEAST REAR. SHOP BUILDING IN DISTANCE. NOTE CONCRETE PROTECTION SLAB FOR UNDERGROUND CONTROL ROOM AND ESCAPE HATCH ON GROUND AT RIGHT MIDDLE DISTANCE. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Firing Control Building, Test Area 1-100, northeast end of Test Area 1-100 Road, Boron, Kern County, CA
HEAT EXCHANGER BUILDING, TRA644. NORTHEAST CORNER. CAMERA IS ON PIKE ...
HEAT EXCHANGER BUILDING, TRA-644. NORTHEAST CORNER. CAMERA IS ON PIKE STREET FACING SOUTHWEST. ATTACHED STRUCTURE AT RIGHT OF VIEW IS ETR COMPRESSOR BUILDING, TRA-643. INL NEGATIVE NO. HD46-36-4. Mike Crane, Photographer, 4/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
11. Historic view of Building 100 control room, showing personnel ...
11. Historic view of Building 100 control room, showing personnel operating rocket engine test controls and observer watching activity from observation room. May 27, 1957. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA photo number C-45020. - Rocket Engine Testing Facility, GRC Building No. 100, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
REACTOR SERVICE BUILDING, TRA635. CROWDED MOCKUP AREA. CAMERA FACES EAST. ...
REACTOR SERVICE BUILDING, TRA-635. CROWDED MOCK-UP AREA. CAMERA FACES EAST. PHOTOGRAPHER'S NOTE SAYS "PICTURE REQUESTED BY IDO IN SUPPORT OF FY '58 BUILDING PROJECTS." INL NEGATIVE NO. 56-3025. R.G. Larsen, Photographer, 9/13/1956 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
FAST CHOPPER BUILDING, TRA665. DETAIL SHOWS UPPER AND LOWER LEVEL ...
FAST CHOPPER BUILDING, TRA-665. DETAIL SHOWS UPPER AND LOWER LEVEL WALLS OF DIFFERING MATERIALS. NOTE DOORWAY TO MTR TO RIGHT OF CHOPPER BUILDING'S CLIPPED CORNER. CAMERA FACING WEST. INL NEGATIVE NO. HD42-1. Mike Crane, Photographer, 3/2004 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
Replacement Windows Define the Newly Renovated Fiterman Hall.
ERIC Educational Resources Information Center
Ladesich, Jim
2000-01-01
Explores the planning and testing involved in successfully replacing windows in a corporate office building converted into an academic building. Inspection procedures and performance testing are examined. (GR)
Thoracic Injury Risk Curves for Rib Deflections of the SID-IIs Build Level D.
Irwin, Annette L; Crawford, Greg; Gorman, David; Wang, Sikui; Mertz, Harold J
2016-11-01
Injury risk curves for SID-IIs thorax and abdomen rib deflections proposed for future NCAP side impact evaluations were developed from tests conducted with the SID-IIs FRG. Since the floating rib guide is known to reduce the magnitude of the peak rib deflections, injury risk curves developed from SID-IIs FRG data are not appropriate for use with SID-IIs build level D. PMHS injury data from three series of sled tests and one series of whole-body drop tests are paired with thoracic rib deflections from equivalent tests with SID-IIs build level D. Where possible, the rib deflections of SID-IIs build level D were scaled to adjust for differences in impact velocity between the PMHS and SID-IIs tests. Injury risk curves developed by the Mertz-Weber modified median rank method are presented and compared to risk curves developed by other parametric and non-parametric methods.
Test Architecture, Test Retrofit
ERIC Educational Resources Information Center
Fulcher, Glenn; Davidson, Fred
2009-01-01
Just like buildings, tests are designed and built for specific purposes, people, and uses. However, both buildings and tests grow and change over time as the needs of their users change. Sometimes, they are also both used for purposes other than those intended in the original designs. This paper explores architecture as a metaphor for language…
Proctor, Caitlin R; Reimann, Mauro; Vriens, Bas; Hammes, Frederik
2017-12-14
Shower hoses offer an excellent bacterial growth environment in close proximity to a critical end-user exposure route within building drinking water plumbing. However, the health risks associated with and processes underlying the development of biofilms in shower hoses are poorly studied. In a global survey, biofilms from 78 shower hoses from 11 countries were characterized in terms of cell concentration (4.1 × 10 4 -5.8 × 10 8 cells/cm 2 ), metal accumulation (including iron, lead, and copper), and microbiome composition (including presence of potential opportunistic pathogens). In countries using disinfectant, biofilms had on average lower cell concentrations and diversity. Metal accumulation (up to 5 μg-Fe/cm 2 , 75 ng-Pb/cm 2 , and 460 ng-Cu/cm 2 ) seemed to be partially responsible for discoloration in biofilms, and likely originated from other pipes upstream in the building. While some genera that may contain potential opportunistic pathogens (Legionella, detected in 21/78 shower hoses) were positively correlated with biofilm cell concentration, others (Mycobacterium, Pseudomonas) had surprisingly non-existent or negative correlations with biofilm cell concentrations. In a controlled study, 15 identical shower hoses were installed for the same time period in the same country, and both stagnant and flowing water samples were collected. Ecological theory of dispersal and selection helped to explain microbiome composition and diversity of different sample types. Shower hose age was related to metal accumulation but not biofilm cell concentration, while frequency of use appeared to influence biofilm cell concentration. This study shows that shower hose biofilms are clearly a critical element of building drinking water plumbing, and a potential target for building drinking water plumbing monitoring. Copyright © 2017 Elsevier Ltd. All rights reserved.
1. Exterior view of Signal Transfer Building (T28A), looking southwest. ...
1. Exterior view of Signal Transfer Building (T-28A), looking southwest. This structure houses controls for propellant transfer, instrumentation for testing, test data transmission receivers, data verification equipment, and centralized utilities for the Systems Integration Laboratory complex. The gantries of the Systems Integration Laboratory Building (T-28) are visible to the rear of this structure. - Air Force Plant PJKS, Systems Integration Laboratory, Signal Transfer Building, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
LPT. Shield test facility assembly and test building (TAN646), south ...
LPT. Shield test facility assembly and test building (TAN-646), south end of EBOR helium wing. Camera facing north. Monorail protrudes from upper-level door. Rust marks on concrete wall are from stack. Metal shed is post-1970 addition. INEEL negative no. HD-40-8-1 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID
7. BUILDING 604F, INTERIOR OF BULL PEN SHOWING TESTING STAND ...
7. BUILDING 604-F, INTERIOR OF BULL PEN SHOWING TESTING STAND AND HEAVY WOOD LINING ON CONCRETE WALLS. STEEL PLATE ABOVE TEST STAND DEFLECTS SHRAPNEL, SCREEN FURTHER HELPS TO CONTAIN PARTICLES. ONLY SMALL EXPLOSIVES WERE TESTED HERE (GRENADES, MINES, BOMB FUZES, ETC.). - Picatinny Arsenal, 600 Area, Test Areas District, State Route 15 near I-80, Dover, Morris County, NJ
NASA Technical Reports Server (NTRS)
Ralph, E. L.; Linder, E. B.
1996-01-01
Solar panel designs that utilize new high-efficiency solar cells and lightweight rigid panel technologies are described. The resulting designs increase the specific power (W/kg) achievable in the near-term and are well suited to meet the demands of higher performance small satellites (smallsats). Advanced solar panel designs have been developed and demonstrated on two NASA SBIR contracts at Applied Solar. The first used 19% efficient, large area (5.5 cm x 6.5 cm) GaAs/Ge solar cells with a lightweight rigid graphite epoxy isogrid substrate configuration. A 1,445 cm(exp 2) coupon was fabricated and tested to demonstrate 60 W/kg with a high potential of achieving 80 W/kg. The second panel design used new 22% efficiency, dual junction GaInP2/GaAs/Ge solar cells combined with a lightweight aluminum core/graphite fiber mesh facesheet substrate. A 1,445 cm(exp 2) coupon was fabricated and tested to demonstrate 105 W/kg with the potential of achieving 115 W/kg. This paper will address the construction details for the GaAs/isogrid and dual-junction GaAs/carbon mesh panel configurations. These are ultimately sized to provide 75 Watts and 119 Watts respectively for smallsats or may be used as modular building blocks for larger systems. GaAs/isogrid and dual-junction GaAs/carbon mesh coupons have been fabricated and tested to successfully demonstrate critical performance parameters and results are also provided here.
NASA Astrophysics Data System (ADS)
Perret-Aebi, Laure-Emmanuelle; Escarré, Jordi; Li, Heng-Yu; Sansonnens, Laurent; Galliano, Federico; Cattaneo, Gianluca; Heinstein, Patrick; Nicolay, Sylvain; Bailat, Julien; Eberhard, Sébastien; Ballif, Christophe
2015-09-01
The use of photovoltaic (PV) is not anymore an option but a real need in the construction of nearly zero energy buildings. To date, the lack of PV products specifically designed for building integration, considering aesthetics and architectural aspects, is one important limiting factor allowing a massive deployment of PV in the built environment. Architects are continuously asking for new solutions to customize the colour of PV elements to better integrate them into the building skin. Among these colours, white is especially attractive as it is widely used in architecture for its elegance, versatility and fresh look. Until now, white solar modules were not considered to be an option and even never been though to be a technological possibility. Nonetheless, CSEM recently developed a new technology to make white solar modules a reality. Furthermore, the new Swiss company called Solaxess is now working on the industrialisation of this very innovative technology and the first products are expecting to be on the market at the end of 2015. The technology is based on the combination of two different elements: a solar cell able to convert solar infrared light into electricity and a selective filter which reflects and diffuse the whole visible spectrum. Any PV technology based on crystalline silicon can be used as they have a good response in the infrared. Approximately 55% of the current generated under standard test conditions comes from the infrared leading to conversion efficiencies above 11%. We will demonstrate, that thanks to this very innovative technology PV modules can become attractive and real active building elements and therefore meets the requirements of any future energy management through advanced building skins.
Genetics Home Reference: cystinosis
... the amino acid cystine (a building block of proteins) within cells. Excess cystine damages cells and often ... gene lead to a deficiency of a transporter protein called cystinosin. Within cells, this protein normally moves ...
LPT. Low power test control building (TAN641) east facade. Sign ...
LPT. Low power test control building (TAN-641) east facade. Sign says "Energy and Systems Technology Laboratory, INEL" (Post-ANP-use). Camera facing west. INEEL negative no. HD-40-3-2 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID
An Experimental Test of Factors Attracting Deer Mice into Buildings.
Kuenzi, Amy J; Douglass, Richard
2009-09-01
Deer mice (Peromyscus maniculatus) are the principal reservoir host of Sin Nombre virus (SNV). Deer mice use a wide variety of habitats including peridomestic settings in and around human dwellings, their presence in and around homes has been implicated as a risk factor for acquiring Hantavirus Pulmonary Syndrome. Deer mice are believed to enter buildings in order to gain access to a variety of resources including food, bedding material, and better thermal microclimates. However, no one has experimentally tested which factors influence mice use of buildings. We conducted experiments using small simulated buildings to determine the effects of two factors, i.e., food and bedding material, on mouse activity in these buildings. We also examined if these effects varied with time of year. We found that deer mice entered our buildings regardless of the presence or absence of food or bedding. However, the amount of activity in buildings was affected by what they contained. We found significantly higher indices of activity in buildings containing food compared to both empty buildings (control) and buildings containing bedding material. Time of year did not affect activity in buildings.
DOE Office of Scientific and Technical Information (OSTI.GOV)
None, None
This report is concerned with the nature and scope of the technical services to be rendered and the general plan proposed for operation of Building 3525, High Radiation Level Examination Laboratory (HRLEL). The role of postirradiation examination in implementing the over- all task of irradiation testing for various programs under way at the Oak Ridge National Laboratory (ORNL) and the importance of this effort to the United Stat es reactor development program are stressed . The shielded-cell complex with provisions for remote decontamination, hot-equipment storage, and maintenance is described, as well as other supporting activities which are incorporated into themore » facility. The proposed technical functions include general observation, mensuration, nondestructive testing, burnup and induced-activity measurements, fission-gas sampling and analysis, corrosion evaluation and related measurements, disassembly and cutup, metallographic examination, mechanical-property determinations , and x -ray diffraction analyses. Equipment design and operational features to improve detection and measurement of selected properties in radioactive material s are described, also. The current status on design, procurement, construction, and preoperational testing of in- cell equipment in the mockup is presented along with a forecast of future needs. The mode of operation, manpower requirements, and management of the facility are discussed.« less
1. Context view of Building 205, showing hydrogen tanks in ...
1. Context view of Building 205, showing hydrogen tanks in foreground and Building 202 exhaust scrubber stack in background. View looking southwest from top of mound east of Building 205. - Rocket Engine Testing Facility, GRC Building No. 205, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
Development of Test Article Building Block (TABB) for deployable platform systems
NASA Technical Reports Server (NTRS)
Greenberg, H. S.; Barbour, R. T.
1984-01-01
The concept of a Test Article Building Block (TABB) is described. The TABB is a ground test article that is representative of a future building block that can be used to construct LEO and GEO deployable space platforms for communications and scientific payloads. This building block contains a main housing within which the entire structure, utilities, and deployment/retraction mechanism are stowed during launch. The end adapter secures the foregoing components to the housing during launch. The main housing and adapter provide the necessary building-block-to-building-block attachments for automatically deployable platforms. Removal from the shuttle cargo bay can be accomplished with the remote manipulator system (RMS) and/or the handling and positioning aid (HAPA). In this concept, all the electrical connections are in place prior to launch with automatic latches for payload attachment provided on either the end adapters or housings. The housings also can contain orbiter docking ports for payload installation and maintenance.
NEET-AMM Final Technical Report on Laser Direct Manufacturing (LDM) for Nuclear Power Components
DOE Office of Scientific and Technical Information (OSTI.GOV)
Anderson, Scott; Baca, Georgina; O'Connor, Michael
2015-12-31
Final technical report summarizes the program progress and technical accomplishments of the Laser Direct Manufacturing (LDM) for Nuclear Power Components project. A series of experiments varying build process parameters (scan speed and laser power) were conducted at the outset to establish the optimal build conditions for each of the alloys. Fabrication was completed in collaboration with Quad City Manufacturing Laboratory (QCML). The density of all sample specimens was measured and compared to literature values. Optimal build process conditions giving fabricated part densities close to literature values were chosen for making mechanical test coupons. Test coupons whose principal axis is onmore » the x-y plane (perpendicular to build direction) and on the z plane (parallel to build direction) were built and tested as part of the experimental build matrix to understand the impact of the anisotropic nature of the process.. Investigations are described 316L SS, Inconel 600, 718 and 800 and oxide dispersion strengthed 316L SS (Yttria) alloys.« less
NASA Astrophysics Data System (ADS)
Wang, Jinxia; Dou, Aixia; Wang, Xiaoqing; Huang, Shusong; Yuan, Xiaoxiang
2016-11-01
Compared to remote sensing image, post-earthquake airborne Light Detection And Ranging (LiDAR) point cloud data contains a high-precision three-dimensional information on earthquake disaster which can improve the accuracy of the identification of destroy buildings. However after the earthquake, the damaged buildings showed so many different characteristics that we can't distinguish currently between trees and damaged buildings points by the most commonly used method of pre-processing. In this study, we analyse the number of returns for given pulse of trees and damaged buildings point cloud and explore methods to distinguish currently between trees and damaged buildings points. We propose a new method by searching for a certain number of neighbourhood space and calculate the ratio(R) of points whose number of returns for given pulse greater than 1 of the neighbourhood points to separate trees from buildings. In this study, we select some point clouds of typical undamaged building, collapsed building and tree as samples from airborne LiDAR point cloud data which got after 2010 earthquake in Haiti MW7.0 by the way of human-computer interaction. Testing to get the Rvalue to distinguish between trees and buildings and apply the R-value to test testing areas. The experiment results show that the proposed method in this study can distinguish between building (undamaged and damaged building) points and tree points effectively but be limited in area where buildings various, damaged complex and trees dense, so this method will be improved necessarily.
PLUG STORAGE BUILDING, TRA611, AWAITS SHIELDING SOIL TO BE PLACED ...
PLUG STORAGE BUILDING, TRA-611, AWAITS SHIELDING SOIL TO BE PLACED OVER PLUG STORAGE TUBES. WING WALLS WILL SUPPORT EARTH FILL. MTR, PROCESS WATER BUILDING, AND WORKING RESERVOIR IN VIEW BEYOND PLUG STORAGE. CAMERA FACES NORTHEAST. INL NEGATIVE NO. 2949. Unknown Photographer, 7/30/1951 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
REACTOR SERVICES BUILDING, TRA635, INTERIOR. ALSO KNOWN AS MATERIAL RECEIVING ...
REACTOR SERVICES BUILDING, TRA-635, INTERIOR. ALSO KNOWN AS MATERIAL RECEIVING AREA AND LABORATORY. CAMERA ON FIRST FLOOR FACING NORTH TOWARD MTR BUILDING. MOCK-UP AREA WAS TO THE RIGHT OF VIEW. INL NEGATIVE NO. HD46-10-1. Mike Crane, Photographer, 2/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
View of camera station located northeast of Building 70022, facing ...
View of camera station located northeast of Building 70022, facing northwest - Naval Ordnance Test Station Inyokern, Randsburg Wash Facility Target Test Towers, Tower Road, China Lake, Kern County, CA
Looking North into Lab Metallurgy Testing Area and Enrichment Motor ...
Looking North into Lab Metallurgy Testing Area and Enrichment Motor within Recycle Recovery Building - Hematite Fuel Fabrication Facility, Recycle Recovery Building, 3300 State Road P, Festus, Jefferson County, MO
View of building 11050. With view of building 11070 (See ...
View of building 11050. With view of building 11070 (See HABS No. CA 2774-B) in background. Looking southwest. - Naval Ordnance Test Station Inyokern, China Lake Pilot Plant, Machine Shop, C Street, China Lake, Kern County, CA
6. Historic photo of rocket engine test facility Building 202 ...
6. Historic photo of rocket engine test facility Building 202 complex in operation at night, September 12, 1957. On file at NASA Plumbrook Research Center, Sandusky, Ohio. NASA GRC photo number C-45924. - Rocket Engine Testing Facility, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH
5. EDGE OF CAPTIVE TEST STAND THREE FERROCEMENT APRON AT ...
5. EDGE OF CAPTIVE TEST STAND THREE FERROCEMENT APRON AT FAR LEFT, CONNECTING TUNNEL AT CENTER, CONTROL BUILDING B AT RIGHT, VIEW TOWARDS SOUTHWEST. - Glenn L. Martin Company, Titan Missile Test Facilities, Control Building B, Waterton Canyon Road & Colorado Highway 121, Lakewood, Jefferson County, CO
Effects of Polyhydroxybutyrate Production on Cell Division
NASA Technical Reports Server (NTRS)
Miller, Kathleen; Rahman, Asif; Hadi, Masood Z.
2015-01-01
Synthetic biological engineering can be utilized to aide the advancement of improved long-term space flight. The potential to use synthetic biology as a platform to biomanufacture desired equipment on demand using the three dimensional (3D) printer on the International Space Station (ISS) gives long-term NASA missions the flexibility to produce materials as needed on site. Polyhydroxybutyrates (PHBs) are biodegradable, have properties similar to plastics, and can be produced in Escherichia coli using genetic engineering. Using PHBs during space flight could assist mission success by providing a valuable source of biomaterials that can have many potential applications, particularly through 3D printing. It is well documented that during PHB production E. coli cells can become significantly elongated. The elongation of cells reduces the ability of the cells to divide and thus to produce PHB. I aim to better understand cell division during PHB production, through the design, building, and testing of synthetic biological circuits, and identify how to potentially increase yields of PHB with FtsZ overexpression, the gene responsible for cell division. Ultimately, an increase in the yield will allow more products to be created using the 3D printer on the ISS and beyond, thus aiding astronauts in their missions.
ETR COMPLEX. CAMERA FACING EAST. FROM LEFT TO RIGHT: ETRCRITICAL ...
ETR COMPLEX. CAMERA FACING EAST. FROM LEFT TO RIGHT: ETR-CRITICAL FACILITY BUILDING, ETR CONTROL BUILDING (ATTACHED TO HIGH-BAY ETR), ETR, ONE-STORY SECTION OF ETR BUILDING, ELECTRICAL BUILDING, COOLING TOWER PUMP HOUSE, COOLING TOWER. COMPRESSOR AND HEAT EXCHANGER BUILDING ARE PARTLY IN VIEW ABOVE ETR. DARK-COLORED DUCTS PROCEED FROM GROUND CONNECTION TO ETR WASTE GAS STACK. OTHER STACK IS MTR STACK WITH FAN HOUSE IN FRONT OF IT. RECTANGULAR STRUCTURE NEAR TOP OF VIEW IS SETTLING BASIN. INL NEGATIVE NO. 56-4102. Unknown Photographer, ca. 1956 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID
View of building 11050 looking southeast. Naval Ordnance Test ...
View of building 11050 looking southeast. - Naval Ordnance Test Station Inyokern, China Lake Pilot Plant, Fire Station & Marine Barracks, D Street, at corner of 4th Street, China Lake, Kern County, CA
10 CFR 431.263 - Materials incorporated by reference.
Code of Federal Regulations, 2011 CFR
2011-01-01
... Society for Testing and Materials (ASTM) Standard F2324-03, “Standard Test Method for Prerinse Spray..., Forrestal Building, Room 1J-018 (Resource Room of the Building Technologies Program), 1000 Independence...
10 CFR 431.263 - Materials incorporated by reference.
Code of Federal Regulations, 2010 CFR
2010-01-01
... Society for Testing and Materials (ASTM) Standard F2324-03, “Standard Test Method for Prerinse Spray..., Forrestal Building, Room 1J-018 (Resource Room of the Building Technologies Program), 1000 Independence...
DOE Office of Scientific and Technical Information (OSTI.GOV)
David A. King
2011-06-27
Building K-33 was constructed in 1954 as the final section of the five-stage uranium enrichment cascade at the Oak Ridge Gaseous Diffusion Plant (ORGDP). The two original building (K-25 and K-27) were used to produce weapons grade highly enriched uranium (HEU). Building K-29, K-31, and K-33 were added to produce low enriched uranium (LEU) for nuclear power plant fuel. During ORGDP operations K-33 produced a peak enrichment of 2.5%. Thousands of tons of reactor tails fed into gaseous diffusion plants in the 1950s and early 1960s introducing some fission products and transuranics. Building K-33 was a two-story, 25-meters (82-feet) tallmore » structure with approximately 30 hectare (64 acres) of floor space. The Operations (first) Floor contained offices, change houses, feed vaporization rooms, and auxiliary equipment to support enrichment operations. The Cell (second) Floor contained the enrichment process equipment and was divided into eight process units (designated K-902-1 through K-902-8). Each unit contained ten cells, and each cell contained eight process stages (diffusers) for a total of 640 enrichment stages. 1985: LEU buildings were taken off-line after the anticipated demand for uranium enrichment failed to materialize. 1987: LEU buildings were placed in permanent shutdown. Process equipment were maintained in a shutdown state. 1997: DOE signed an Action Memorandum for equipment removal and decontamination of Buildings K-29, K-31, K-33; BNFL awarded contract to reindustrialize the buildings under the Three Buildings D&D and Recycle Project. 2002: Equipment removal complete and effort shifts to vacuuming, chemical cleaning, scabbling, etc. 2005: Decontamination efforts in K-33 cease. Building left with significant {sup 99}Tc contamination on metal structures and PCB contamination in concrete. Uranium, transuranics, and fission products also present on building shell. 2009: DOE targets Building K-33 for demolition. 2010: ORAU contracted to characterize Building K-33 for final disposition at the Environmental Management Waste Management Facility (EMWMF) in Oak Ridge. ORAU collected 439 samples from May and June. LATA Sharp started removing transite panels in September. 2011: LATA Sharp began demolition in January and expects the last waste shipment to EMWMF in September. Approximately 237,000 m{sup 3} (310,000 yd{sup 3}, bulked) of waste taken to EMWMF in 23,000 truckloads expected by project completion.« less
OUT Success Stories: Solar Roofing Shingles
DOE R&D Accomplishments Database
Johnson, N.
2000-08-01
Thin-film photovoltaic (PV) cells are now doubling as rooftop shingles. PV shingles offer many advantages. The energy generated from a building's PV rooftop shingles can provide power both to the building and the utility's power grid.
2014-06-06
CAPE CANAVERAL, Fla. -- Inside the Operations and Checkout Building high bay at NASA's Kennedy Space Center in Florida, NASA and Lockheed Martin engineers and technicians monitor the progress as a crane is used to move the Orion service module to the Final Assembly and System Testing, or FAST, cell further down the aisle. The Orion crew module will be stacked on the service module in the FAST cell and then both modules will be put through their final system tests for Exploration Flight Test-1, or EFT-1, before rolling out of the facility for integration with the United Launch Alliance Delta IV Heavy rocket. Orion is the exploration spacecraft designed to carry astronauts to destinations not yet explored by humans, including an asteroid and Mars. It will have emergency abort capability, sustain the crew during space travel and provide safe re-entry from deep space return velocities. The first unpiloted test flight of Orion, EFT-1, is scheduled to launch later this year atop a Delta IV rocket from Cape Canaveral Air Force Station in Florida to an altitude of 3,600 miles above the Earth's surface. The two-orbit, four-hour flight test will help engineers evaluate the systems critical to crew safety including the heat shield, parachute system and launch abort system. For more information, visit http://www.nasa.gov/orion. Photo credit: NASA/Glenn Benson
2014-06-06
CAPE CANAVERAL, Fla. -- Inside the Operations and Checkout Building high bay at NASA's Kennedy Space Center in Florida, NASA and Lockheed Martin engineers and technicians monitor the progress as a crane is used to move the Orion service module to the Final Assembly and System Testing, or FAST, cell further down the aisle. The Orion crew module will be stacked on the service module in the FAST cell and then both modules will be put through their final system tests for Exploration Flight Test-1, or EFT-1, before rolling out of the facility for integration with the United Launch Alliance Delta IV Heavy rocket. Orion is the exploration spacecraft designed to carry astronauts to destinations not yet explored by humans, including an asteroid and Mars. It will have emergency abort capability, sustain the crew during space travel and provide safe re-entry from deep space return velocities. The first unpiloted test flight of Orion, EFT-1, is scheduled to launch later this year atop a Delta IV rocket from Cape Canaveral Air Force Station in Florida to an altitude of 3,600 miles above the Earth's surface. The two-orbit, four-hour flight test will help engineers evaluate the systems critical to crew safety including the heat shield, parachute system and launch abort system. For more information, visit http://www.nasa.gov/orion. Photo credit: NASA/Glenn Benson
Jang, Sumin; Choubey, Sandeep; Furchtgott, Leon; Zou, Ling-Nan; Doyle, Adele; Menon, Vilas; Loew, Ethan B; Krostag, Anne-Rachel; Martinez, Refugio A; Madisen, Linda; Levi, Boaz P; Ramanathan, Sharad
2017-01-01
The complexity of gene regulatory networks that lead multipotent cells to acquire different cell fates makes a quantitative understanding of differentiation challenging. Using a statistical framework to analyze single-cell transcriptomics data, we infer the gene expression dynamics of early mouse embryonic stem (mES) cell differentiation, uncovering discrete transitions across nine cell states. We validate the predicted transitions across discrete states using flow cytometry. Moreover, using live-cell microscopy, we show that individual cells undergo abrupt transitions from a naïve to primed pluripotent state. Using the inferred discrete cell states to build a probabilistic model for the underlying gene regulatory network, we further predict and experimentally verify that these states have unique response to perturbations, thus defining them functionally. Our study provides a framework to infer the dynamics of differentiation from single cell transcriptomics data and to build predictive models of the gene regulatory networks that drive the sequence of cell fate decisions during development. DOI: http://dx.doi.org/10.7554/eLife.20487.001 PMID:28296635
A gene profiling deconvolution approach to estimating immune cell composition from complex tissues.
Chen, Shu-Hwa; Kuo, Wen-Yu; Su, Sheng-Yao; Chung, Wei-Chun; Ho, Jen-Ming; Lu, Henry Horng-Shing; Lin, Chung-Yen
2018-05-08
A new emerged cancer treatment utilizes intrinsic immune surveillance mechanism that is silenced by those malicious cells. Hence, studies of tumor infiltrating lymphocyte populations (TILs) are key to the success of advanced treatments. In addition to laboratory methods such as immunohistochemistry and flow cytometry, in silico gene expression deconvolution methods are available for analyses of relative proportions of immune cell types. Herein, we used microarray data from the public domain to profile gene expression pattern of twenty-two immune cell types. Initially, outliers were detected based on the consistency of gene profiling clustering results and the original cell phenotype notation. Subsequently, we filtered out genes that are expressed in non-hematopoietic normal tissues and cancer cells. For every pair of immune cell types, we ran t-tests for each gene, and defined differentially expressed genes (DEGs) from this comparison. Equal numbers of DEGs were then collected as candidate lists and numbers of conditions and minimal values for building signature matrixes were calculated. Finally, we used v -Support Vector Regression to construct a deconvolution model. The performance of our system was finally evaluated using blood biopsies from 20 adults, in which 9 immune cell types were identified using flow cytometry. The present computations performed better than current state-of-the-art deconvolution methods. Finally, we implemented the proposed method into R and tested extensibility and usability on Windows, MacOS, and Linux operating systems. The method, MySort, is wrapped as the Galaxy platform pluggable tool and usage details are available at https://testtoolshed.g2.bx.psu.edu/view/moneycat/mysort/e3afe097e80a .
1999-02-17
Various materials are ready for testing in the Kennedy Space Center's cryogenics test bed laboratory. The cryogenics laboratory is expanding to a larger test bed facility in order to offer research and development capabilities that will benefit projects originating from KSC, academia and private industry. Located in KSC's industrial area, the lab is equipped with a liquid nitrogen flow test area to test and evaluate cryogenic valves, flow-meters and other handling equipment in field conditions. A 6,000-gallon tank supplies liquid to low-flow and high-flow test sections. KSC engineers and scientists can also build system prototypes and then field test and analyze them with the center's unique equipment. Expanded cryogenic infrastructure will posture the Space Coast to support biological and medical researchers who use liquid nitrogen to preserve and store human and animal cells and to destroy cancer tissue using cryosurgery; hospitals that use superconductive magnets cooled in liquid helium for magnetic resonance imaging (MRI); the food industry, which uses liquid nitrogen for freezing and long-term storage; as well as the next generation of reusable launch vehicles currently in development
1999-02-17
Materials are being tested in the Kennedy Space Center's cryogenics test bed laboratory. The cryogenics laboratory is expanding to a larger test bed facility in order to offer research and development capabilities that will benefit projects originating from KSC, academia and private industry. Located in KSC's industrial area, the lab is equipped with a liquid nitrogen flow test area to test and evaluate cryogenic valves, flow-meters and other handling equipment in field conditions. A 6,000-gallon tank supplies liquid to low-flow and high-flow test sections. KSC engineers and scientists can also build system prototypes and then field test and analyze them with the center's unique equipment. Expanded cryogenic infrastructure will posture the Space Coast to support biological and medical researchers who use liquid nitrogen to preserve and store human and animal cells and to destroy cancer tissue using cryosurgery; hospitals that use superconductive magnets cooled in liquid helium for magnetic resonance imaging (MRI); the food industry, which uses liquid nitrogen for freezing and long-term storage; as well as the next generation of reusable launch vehicles currently in development
LPT. Shield test control building (TAN645), north facade. Camera facing ...
LPT. Shield test control building (TAN-645), north facade. Camera facing south. Obsolete sign dating from post-1970 program says "Energy and Systems Technology Experimental Facility, INEL." INEEL negative no. HD-40-5-4 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID
Shake Table Testing of an Elevator System in a Full-Scale Five-Story Building
Wang, Xiang; Hutchinson, Tara C.; Astroza, Rodrigo; Conte, Joel P.; Restrepo, José I.; Hoehler, Matthew S.; Ribeiro, Waldir
2016-01-01
SUMMARY This paper investigates the seismic performance of a functional traction elevator as part of a full-scale five-story building shake table test program. The test building was subjected to a suite of earthquake input motions of increasing intensity, first while the building was isolated at its base, and subsequently while it was fixed to the shake table platen. In addition, low-amplitude white noise base excitation tests were conducted while the elevator system was placed in three different configurations, namely, by varying the vertical location of its cabin and counterweight, to study the acceleration amplifications of the elevator components due to dynamic excitations. During the earthquake tests, detailed observation of the physical damage and operability of the elevator as well as its measured response are reported. Although the cabin and counterweight sustained large accelerations due to impact during these tests, the use of well-restrained guide shoes demonstrated its effectiveness in preventing the cabin and counterweight from derailment during high-intensity earthquake shaking. However, differential displacements induced by the building imposed undesirable distortion of the elevator components and their surrounding support structure, which caused damage and inoperability of the elevator doors. It is recommended that these aspects be explicitly considered in elevator seismic design. PMID:28242957
Shake Table Testing of an Elevator System in a Full-Scale Five-Story Building.
Wang, Xiang; Hutchinson, Tara C; Astroza, Rodrigo; Conte, Joel P; Restrepo, José I; Hoehler, Matthew S; Ribeiro, Waldir
2017-03-01
This paper investigates the seismic performance of a functional traction elevator as part of a full-scale five-story building shake table test program. The test building was subjected to a suite of earthquake input motions of increasing intensity, first while the building was isolated at its base, and subsequently while it was fixed to the shake table platen. In addition, low-amplitude white noise base excitation tests were conducted while the elevator system was placed in three different configurations, namely, by varying the vertical location of its cabin and counterweight, to study the acceleration amplifications of the elevator components due to dynamic excitations. During the earthquake tests, detailed observation of the physical damage and operability of the elevator as well as its measured response are reported. Although the cabin and counterweight sustained large accelerations due to impact during these tests, the use of well-restrained guide shoes demonstrated its effectiveness in preventing the cabin and counterweight from derailment during high-intensity earthquake shaking. However, differential displacements induced by the building imposed undesirable distortion of the elevator components and their surrounding support structure, which caused damage and inoperability of the elevator doors. It is recommended that these aspects be explicitly considered in elevator seismic design.
SUNREL Applications | Buildings | NREL
used by the building design team for energy analysis. Zion National Park Visitor's Center Grand Canyon National Park Bookstore NREL Thermal Test Facility NREL Wind Site Entrance Building DPD Office Building Performance Analysis of a High-Mass Residential Building Van Geet residence The Van Geet Off-Grid Home: An
Electrolyser and fuel cells, key elements for energy and life support
NASA Astrophysics Data System (ADS)
Bockstahler, Klaus; Funke, Helmut; Lucas, Joachim
Both, Electrolyser and Fuel Cells are key elements for regenerative energy and life support systems. Electrolyser technology is originally intended for oxygen production in manned space habitats and in submarines, through splitting water into hydrogen and oxygen. Fuel cells serve for energy production through the reaction, triggered in the presence of an electrolyte, between a fuel and an oxidant. Now combining both technologies i.e. electrolyser and fuel cell makes it a Regenerative Fuel Cell System (RFCS). In charge mode, i.e. with energy supplied e.g. by solar cells, the electrolyser splits water into hydrogen and oxygen being stored in tanks. In discharge mode, when power is needed but no energy is available, the stored gases are converted in the fuel cell to generate electricity under the formation of water that is stored in tanks. Rerouting the water to the electrolyser makes it a closed-loop i.e. regenerative process. Different electrolyser and fuel cell technologies are being evolved. At Astrium emphasis is put on the development of an RFCS comprised of Fixed Alkaline Electrolyser (FAE) and Fuel Cell (AFC) as such technology offers a high electrical efficiency and thus reduced system weight, which is important in space applications. With increasing power demand and increasing discharge time an RFCS proves to be superior to batteries. Since the early technology development multiple design refinements were done at Astrium, funded by the European Space Agency ESA and the German National Agency DLR as well as based on company internal R and T funding. Today a complete RFCS energy system breadboard is established and the operational behavior of the system is being tested. In parallel the electrolyser itself is subject to design refinement and testing in terms of oxygen production in manned space habitats. In addition essential features and components for process monitoring and control are being developed. The present results and achievements and the dedicated experience gained from testing will be presented, complemented by an outlook on next development steps preparatory to the application of electrolyser and fuel cell technology in human and robotic exploration building blocks.
High Performance Building Mockup in FLEXLAB
DOE Office of Scientific and Technical Information (OSTI.GOV)
McNeil, Andrew; Kohler, Christian; Lee, Eleanor S.
Genentech has ambitious energy and indoor environmental quality performance goals for Building 35 (B35) being constructed by Webcor at the South San Francisco campus. Genentech and Webcor contracted with the Lawrence Berkeley National Laboratory (LBNL) to test building systems including lighting, lighting controls, shade fabric, and automated shading controls in LBNL’s new FLEXLAB facility. The goal of the testing is to ensure that the systems installed in the new office building will function in a way that reduces energy consumption and provides a comfortable work environment for employees.
PROCESS WATER BUILDING, TRA605. AERIAL TAKEN WHILE SEVERAL PIPE TRENCHES ...
PROCESS WATER BUILDING, TRA-605. AERIAL TAKEN WHILE SEVERAL PIPE TRENCHES REMAINED OPEN. CAMERA FACES EASTERLY. NOTE DUAL PIPES BETWEEN REACTOR BUILDING AND NORTH SIDE OF PROCESS WATER BUILDING. PIPING NEAR WORKING RESERVOIR HEADS FOR RETENTION RESERVOIR. PIPE FROM DEMINERALIZER ENTERS MTR FROM NORTH. SEE ALSO TRENCH FOR COOLANT AIR DUCT AT SOUTH SIDE OF MTR AND LEADING TO FAN HOUSE AND STACK. INL NEGATIVE NO. 2966-A. Unknown Photographer, 7/31/1951 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID