Diffraction mode terahertz tomography

DOEpatents

Ferguson, Bradley; Wang, Shaohong; Zhang, Xi-Cheng

2006-10-31

A method of obtaining a series of images of a three-dimensional object. The method includes the steps of transmitting pulsed terahertz (THz) radiation through the entire object from a plurality of angles, optically detecting changes in the transmitted THz radiation using pulsed laser radiation, and constructing a plurality of imaged slices of the three-dimensional object using the detected changes in the transmitted THz radiation. The THz radiation is transmitted through the object as a two-dimensional array of parallel rays. The optical detection is an array of detectors such as a CCD sensor.

Three-dimensional multifunctional optical coherence tomography for skin imaging

NASA Astrophysics Data System (ADS)

Li, En; Makita, Shuichi; Hong, Young-Joo; Kasaragod, Deepa; Sasaoka, Tomoko; Yamanari, Masahiro; Sugiyama, Satoshi; Yasuno, Yoshiaki

2016-02-01

Optical coherence tomography (OCT) visualizes cross-sectional microstructures of biological tissues. Recent developments of multifunctional OCT (MF-OCT) provides multiple optical contrasts which can reveal currently unknown tissue properties. In this contribution we demonstrate multifunctional OCT specially designed for dermatological investigation. And by utilizing it to measure four different body parts of in vivo human skin, three-dimensional scattering OCT, OCT angiography, polarization uniformity tomography, and local birefringence tomography images were obtained by a single scan. They respectively contrast the structure and morphology, vasculature, melanin content and collagen traits of the tissue.

Three-dimensional confocal microscopy of the living cornea and ocular lens

NASA Astrophysics Data System (ADS)

Masters, Barry R.

1991-07-01

The three-dimensional reconstruction of the optic zone of the cornea and the ocular crystalline lens has been accomplished using confocal microscopy and volume rendering computer techniques. A laser scanning confocal microscope was used in the reflected light mode to obtain the two-dimensional images from the cornea and the ocular lens of a freshly enucleated rabbit eye. The light source was an argon ion laser with a 488 nm wavelength. The microscope objective was a Leitz X25, NA 0.6 water immersion lens. The 400 micron thick cornea was optically sectioned into 133 three micron sections. The semi-transparent cornea and the in-situ ocular lens was visualized as high resolution, high contrast two-dimensional images. The structures observed in the cornea include: superficial epithelial cells and their nuclei, basal epithelial cells and their 'beaded' cell borders, basal lamina, nerve plexus, nerve fibers, nuclei of stromal keratocytes, and endothelial cells. The structures observed in the in- situ ocular lens include: lens capsule, lens epithelial cells, and individual lens fibers. The three-dimensional data sets of the cornea and the ocular lens were reconstructed in the computer using volume rendering techniques. Stereo pairs were also created of the two- dimensional ocular images for visualization. The stack of two-dimensional images was reconstructed into a three-dimensional object using volume rendering techniques. This demonstration of the three-dimensional visualization of the intact, enucleated eye provides an important step toward quantitative three-dimensional morphometry of the eye. The important aspects of three-dimensional reconstruction are discussed.

Three-dimensional image formation in fiber-optical second-harmonic-generation microscopy.

PubMed

Gu, Min; Fu, Ling

2006-02-06

Three-dimensional (3-D) image formation in fiber-optical second-harmonic-generation microscopy is revealed to be purely coherent and therefore can be described by a 3-D coherent transfer function (CTF) that exhibits the same spatial frequency passband as that of fiber-optical reflection-mode non-fluorescence microscopy. When the numerical aperture of the fiber is much larger than the angle of convergence of the illumination on the fiber aperture, the performance of fiber-optical second-harmonic-generation microscopy behaves as confocal second-harmonic-generation microscopy. The dependence of axial resolution on fiber coupling parameters shows an improvement of approximately 7%, compared with that in fiber-optical two-photon fluorescence microscopy.

LED-based interference-reflection microscopy combined with optical tweezers for quantitative three-dimensional microtubule imaging.

PubMed

Simmert, Steve; Abdosamadi, Mohammad Kazem; Hermsdorf, Gero; Schäffer, Erik

2018-05-28

Optical tweezers combined with various microscopy techniques are a versatile tool for single-molecule force spectroscopy. However, some combinations may compromise measurements. Here, we combined optical tweezers with total-internal-reflection-fluorescence (TIRF) and interference-reflection microscopy (IRM). Using a light-emitting diode (LED) for IRM illumination, we show that single microtubules can be imaged with high contrast. Furthermore, we converted the IRM interference pattern of an upward bent microtubule to its three-dimensional (3D) profile calibrated against the optical tweezers and evanescent TIRF field. In general, LED-based IRM is a powerful method for high-contrast 3D microscopy.

Velocity filtering applied to optical flow calculations

NASA Technical Reports Server (NTRS)

Barniv, Yair

1990-01-01

Optical flow is a method by which a stream of two-dimensional images obtained from a forward-looking passive sensor is used to map the three-dimensional volume in front of a moving vehicle. Passive ranging via optical flow is applied here to the helicopter obstacle-avoidance problem. Velocity filtering is used as a field-based method to determine range to all pixels in the initial image. The theoretical understanding and performance analysis of velocity filtering as applied to optical flow is expanded and experimental results are presented.

Investigation of the relative orientation of the system of optical sensors to monitor the technosphere objects

NASA Astrophysics Data System (ADS)

Petrochenko, Andrey; Konyakhin, Igor

2017-06-01

In connection with the development of robotics have become increasingly popular variety of three-dimensional reconstruction of the system mapping and image-set received from the optical sensors. The main objective of technical and robot vision is the detection, tracking and classification of objects of the space in which these systems and robots operate [15,16,18]. Two-dimensional images sometimes don't contain sufficient information to address those or other problems: the construction of the map of the surrounding area for a route; object identification, tracking their relative position and movement; selection of objects and their attributes to complement the knowledge base. Three-dimensional reconstruction of the surrounding space allows you to obtain information on the relative positions of objects, their shape, surface texture. Systems, providing training on the basis of three-dimensional reconstruction of the results of the comparison can produce two-dimensional images of three-dimensional model that allows for the recognition of volume objects on flat images. The problem of the relative orientation of industrial robots with the ability to build threedimensional scenes of controlled surfaces is becoming actual nowadays.

Space-based optical image encryption.

PubMed

Chen, Wen; Chen, Xudong

2010-12-20

In this paper, we propose a new method based on a three-dimensional (3D) space-based strategy for the optical image encryption. The two-dimensional (2D) processing of a plaintext in the conventional optical encryption methods is extended to a 3D space-based processing. Each pixel of the plaintext is considered as one particle in the proposed space-based optical image encryption, and the diffraction of all particles forms an object wave in the phase-shifting digital holography. The effectiveness and advantages of the proposed method are demonstrated by numerical results. The proposed method can provide a new optical encryption strategy instead of the conventional 2D processing, and may open up a new research perspective for the optical image encryption.

Joint Applied Optics and Chinese Optics Letters feature introduction: digital holography and three-dimensional imaging.

PubMed

Poon, Ting-Chung

2011-12-01

This feature issue serves as a pilot issue promoting the joint issue of Applied Optics and Chinese Optics Letters. It focuses upon topics of current relevance to the community working in the area of digital holography and 3-D imaging. © 2011 Optical Society of America

Biodynamic profiling of three-dimensional tissue growth techniques

NASA Astrophysics Data System (ADS)

Sun, Hao; Merrill, Dan; Turek, John; Nolte, David

2016-03-01

Three-dimensional tissue culture presents a more biologically relevant environment in which to perform drug development than conventional two-dimensional cell culture. However, obtaining high-content information from inside three dimensional tissue has presented an obstacle to rapid adoption of 3D tissue culture for pharmaceutical applications. Biodynamic imaging is a high-content three-dimensional optical imaging technology based on low-coherence interferometry and digital holography that uses intracellular dynamics as high-content image contrast. In this paper, we use biodynamic imaging to compare pharmaceutical responses to Taxol of three-dimensional multicellular spheroids grown by three different growth techniques: rotating bioreactor, hanging-drop and plate-grown spheroids. The three growth techniques have systematic variations among tissue cohesiveness and intracellular activity and consequently display different pharmacodynamics under identical drug dose conditions. The in vitro tissue cultures are also compared to ex vivo living biopsies. These results demonstrate that three-dimensional tissue cultures are not equivalent, and that drug-response studies must take into account the growth method.

Dental image replacement on cone beam computed tomography with three-dimensional optical scanning of a dental cast, occlusal bite, or bite tray impression.

PubMed

Kang, S-H; Lee, J-W; Lim, S-H; Kim, Y-H; Kim, M-K

2014-10-01

The goal of the present study was to compare the accuracy of dental image replacement on a cone beam computed tomography (CBCT) image using digital image data from three-dimensional (3D) optical scanning of a dental cast, occlusal bite, and bite tray impression. A Bracket Typodont dental model was used. CBCT of the dental model was performed and the data were converted to stereolithography (STL) format. Three experimental materials, a dental cast, occlusal bite, and bite tray impression, were optically scanned in 3D. STL files converted from the CBCT of the Typodont model and the 3D optical-scanned STL files of the study materials were image-registered. The error range of each methodology was measured and compared with a 3D optical scan of the Typodont. For the three materials, the smallest error observed was 0.099±0.114mm (mean error±standard deviation) for registering the 3D optical scan image of the dental cast onto the CBCT dental image. Although producing a dental cast can be laborious, the study results indicate that it is the preferred method. In addition, an occlusal bite is recommended when bite impression materials are used. Copyright © 2014 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

Diffuse optical correlation tomography of cerebral blood flow during cortical spreading depression in rat brain

NASA Astrophysics Data System (ADS)

Zhou, Chao; Yu, Guoqiang; Furuya, Daisuke; Greenberg, Joel; Yodh, Arjun; Durduran, Turgut

2006-02-01

Diffuse optical correlation methods were adapted for three-dimensional (3D) tomography of cerebral blood flow (CBF) in small animal models. The image reconstruction was optimized using a noise model for diffuse correlation tomography which enabled better data selection and regularization. The tomographic approach was demonstrated with simulated data and during in-vivo cortical spreading depression (CSD) in rat brain. Three-dimensional images of CBF were obtained through intact skull in tissues(~4mm) deep below the cortex.

Integral image rendering procedure for aberration correction and size measurement.

PubMed

Sommer, Holger; Ihrig, Andreas; Ebenau, Melanie; Flühs, Dirk; Spaan, Bernhard; Eichmann, Marion

2014-05-20

The challenge in rendering integral images is to use as much information preserved by the light field as possible to reconstruct a captured scene in a three-dimensional way. We propose a rendering algorithm based on the projection of rays through a detailed simulation of the optical path, considering all the physical properties and locations of the optical elements. The rendered images contain information about the correct size of imaged objects without the need to calibrate the imaging device. Additionally, aberrations of the optical system may be corrected, depending on the setup of the integral imaging device. We show simulation data that illustrates the aberration correction ability and experimental data from our plenoptic camera, which illustrates the capability of our proposed algorithm to measure size and distance. We believe this rendering procedure will be useful in the future for three-dimensional ophthalmic imaging of the human retina.

An improved three-dimensional non-scanning laser imaging system based on digital micromirror device

NASA Astrophysics Data System (ADS)

Xia, Wenze; Han, Shaokun; Lei, Jieyu; Zhai, Yu; Timofeev, Alexander N.

2018-01-01

Nowadays, there are two main methods to realize three-dimensional non-scanning laser imaging detection, which are detection method based on APD and detection method based on Streak Tube. However, the detection method based on APD possesses some disadvantages, such as small number of pixels, big pixel interval and complex supporting circuit. The detection method based on Streak Tube possesses some disadvantages, such as big volume, bad reliability and high cost. In order to resolve the above questions, this paper proposes an improved three-dimensional non-scanning laser imaging system based on Digital Micromirror Device. In this imaging system, accurate control of laser beams and compact design of imaging structure are realized by several quarter-wave plates and a polarizing beam splitter. The remapping fiber optics is used to sample the image plane of receiving optical lens, and transform the image into line light resource, which can realize the non-scanning imaging principle. The Digital Micromirror Device is used to convert laser pulses from temporal domain to spatial domain. The CCD with strong sensitivity is used to detect the final reflected laser pulses. In this paper, we also use an algorithm which is used to simulate this improved laser imaging system. In the last, the simulated imaging experiment demonstrates that this improved laser imaging system can realize three-dimensional non-scanning laser imaging detection.

Stereo Imaging Velocimetry

NASA Technical Reports Server (NTRS)

McDowell, Mark (Inventor); Glasgow, Thomas K. (Inventor)

1999-01-01

A system and a method for measuring three-dimensional velocities at a plurality of points in a fluid employing at least two cameras positioned approximately perpendicular to one another. The cameras are calibrated to accurately represent image coordinates in world coordinate system. The two-dimensional views of the cameras are recorded for image processing and centroid coordinate determination. Any overlapping particle clusters are decomposed into constituent centroids. The tracer particles are tracked on a two-dimensional basis and then stereo matched to obtain three-dimensional locations of the particles as a function of time so that velocities can be measured therefrom The stereo imaging velocimetry technique of the present invention provides a full-field. quantitative, three-dimensional map of any optically transparent fluid which is seeded with tracer particles.

Real-time spectral imaging in three spatial dimensions

NASA Astrophysics Data System (ADS)

Liu, Wenhai; Psaltis, Demetri; Barbastathis, George

2002-05-01

We report what is to our knowledge the first volume-holographic optical imaging instrument with the capability to return three-dimensional spatial as well as spectral information about semitranslucent microscopic objects in a single measurement. The four-dimensional volume-holographic microscope is characterized theoretically and experimentally by use of fluorescent microspheres as objects.

Super long viewing distance light homogeneous emitting three-dimensional display

NASA Astrophysics Data System (ADS)

Liao, Hongen

2015-04-01

Three-dimensional (3D) display technology has continuously been attracting public attention with the progress in today's 3D television and mature display technologies. The primary characteristics of conventional glasses-free autostereoscopic displays, such as spatial resolution, image depths, and viewing angle, are often limited due to the use of optical lenses or optical gratings. We present a 3D display using MEMS-scanning-mechanism-based light homogeneous emitting (LHE) approach and demonstrate that the display can directly generate an autostereoscopic 3D image without the need for optical lenses or gratings. The generated 3D image has the advantages of non-aberration and a high-definition spatial resolution, making it the first to exhibit animated 3D images with image depth of six meters. Our LHE 3D display approach can be used to generate a natural flat-panel 3D display with super long viewing distance and alternative real-time image update.

Fabrication of 2D and 3D photonic structures using laser lithography

NASA Astrophysics Data System (ADS)

Gaso, P.; Jandura, D.; Pudis, D.

2016-12-01

In this paper we demonstrate possibilities of three-dimensional (3D) printing technology based on two photon polymerization. We used three-dimensional dip-in direct-laser-writing (DLW) optical lithography to fabricate 2D and 3D optical structures for optoelectronics and for optical sensing applications. DLW lithography allows us use a non conventional way how to couple light into the waveguide structure. We prepared ring resonator and we investigated its transmission spectral characteristic. We present 3D inverse opal structure from its design to printing and scanning electron microscope (SEM) imaging. Finally, SEM images of some prepared photonic crystal structures were performed.

Identification of muscle necrosis in the mdx mouse model of Duchenne muscular dystrophy using three-dimensional optical coherence tomography

NASA Astrophysics Data System (ADS)

Klyen, Blake R.; Shavlakadze, Thea; Radley-Crabb, Hannah G.; Grounds, Miranda D.; Sampson, David D.

2011-07-01

Three-dimensional optical coherence tomography (3D-OCT) was used to image the structure and pathology of skeletal muscle tissue from the treadmill-exercised mdx mouse model of human Duchenne muscular dystrophy. Optical coherence tomography (OCT) images of excised muscle samples were compared with co-registered hematoxylin and eosin-stained and Evans blue dye fluorescence histology. We show, for the first time, structural 3D-OCT images of skeletal muscle dystropathology well correlated with co-located histology. OCT could identify morphological features of interest and necrotic lesions within the muscle tissue samples based on intrinsic optical contrast. These findings demonstrate the utility of 3D-OCT for the evaluation of small-animal skeletal muscle morphology and pathology, particularly for studies of mouse models of muscular dystrophy.

Temporal focusing microscopy combined with three-dimensional structured illumination

NASA Astrophysics Data System (ADS)

Isobe, Keisuke; Toda, Keisuke; Song, Qiyuan; Kannari, Fumihiko; Kawano, Hiroyuki; Miyawaki, Atsushi; Midorikawa, Katsumi

2017-05-01

Temporal focusing microscopy provides the optical sectioning capability in wide-field two-photon fluorescence imaging. Here, we demonstrate temporal focusing microscopy combined with three-dimensional structured illumination, which enables us to enhance the three-dimensional spatial resolution and reject the background fluorescence. Experimentally, the periodic pattern of the illumination was produced not only in the lateral direction but also in the axial direction by the interference between three temporal focusing pulses, which were easily generated using a digital micromirror device. The lateral resolution and optical sectioning capability were successfully enhanced by factors of 1.6 and 3.6, respectively, compared with those of temporal focusing microscopy. In the two-photon fluorescence imaging of a tissue-like phantom, the out-of-focus background fluorescence and the scattered background fluorescence could also be rejected.

Observation of three-dimensional internal structure of steel materials by means of serial sectioning with ultrasonic elliptical vibration cutting.

PubMed

Fujisaki, K; Yokota, H; Nakatsuchi, H; Yamagata, Y; Nishikawa, T; Udagawa, T; Makinouchi, A

2010-01-01

A three-dimensional (3D) internal structure observation system based on serial sectioning was developed from an ultrasonic elliptical vibration cutting device and an optical microscope combined with a high-precision positioning device. For bearing steel samples, the cutting device created mirrored surfaces suitable for optical metallography, even for long-cutting distances during serial sectioning of these ferrous materials. Serial sectioning progressed automatically by means of numerical control. The system was used to observe inclusions in steel materials on a scale of several tens of micrometers. Three specimens containing inclusions were prepared from bearing steels. These inclusions could be detected as two-dimensional (2D) sectional images with resolution better than 1 mum. A three-dimensional (3D) model of each inclusion was reconstructed from the 2D serial images. The microscopic 3D models had sharp edges and complicated surfaces.

Multimodal imaging system for dental caries detection

NASA Astrophysics Data System (ADS)

Liang, Rongguang; Wong, Victor; Marcus, Michael; Burns, Peter; McLaughlin, Paul

2007-02-01

Dental caries is a disease in which minerals of the tooth are dissolved by surrounding bacterial plaques. A caries process present for some time may result in a caries lesion. However, if it is detected early enough, the dentist and dental professionals can implement measures to reverse and control caries. Several optical, nonionized methods have been investigated and used to detect dental caries in early stages. However, there is not a method that can singly detect the caries process with both high sensitivity and high specificity. In this paper, we present a multimodal imaging system that combines visible reflectance, fluorescence, and Optical Coherence Tomography (OCT) imaging. This imaging system is designed to obtain one or more two-dimensional images of the tooth (reflectance and fluorescence images) and a three-dimensional OCT image providing depth and size information of the caries. The combination of two- and three-dimensional images of the tooth has the potential for highly sensitive and specific detection of dental caries.

Differentiation of Benign and Malignant Breast Tumors by In-Vivo Three-Dimensional Parallel-Plate Diffuse Optical Tomography

PubMed Central

Choe, Regine; Konecky, Soren D.; Corlu, Alper; Lee, Kijoon; Durduran, Turgut; Busch, David R.; Pathak, Saurav; Czerniecki, Brian J.; Tchou, Julia; Fraker, Douglas L.; DeMichele, Angela; Chance, Britton; Arridge, Simon R.; Schweiger, Martin; Culver, Joseph P.; Schnall, Mitchell D.; Putt, Mary E.; Rosen, Mark A.; Yodh, Arjun G.

2009-01-01

We have developed a novel parallel-plate diffuse optical tomography (DOT) system for three-dimensional in vivo imaging of human breast tumor based on large optical data sets. Images of oxy-, deoxy-, total-hemoglobin concentration, blood oxygen saturation, and tissue scattering were reconstructed. Tumor margins were derived using the optical data with guidance from radiology reports and Magnetic Resonance Imaging. Tumor-to-normal ratios of these endogenous physiological parameters and an optical index were computed for 51 biopsy-proven lesions from 47 subjects. Malignant cancers (N=41) showed statistically significant higher total hemoglobin, oxy-hemoglobin concentration, and scattering compared to normal tissue. Furthermore, malignant lesions exhibited a two-fold average increase in optical index. The influence of core biopsy on DOT results was also explored; the difference between the malignant group measured before core biopsy and the group measured more than one week after core biopsy was not significant. Benign tumors (N=10) did not exhibit statistical significance in the tumor-to-normal ratios of any parameter. Optical index and tumor-to-normal ratios of total hemoglobin, oxy-hemoglobin concentration, and scattering exhibited high area under the receiver operating characteristic curve values from 0.90 to 0.99, suggesting good discriminatory power. The data demonstrate that benign and malignant lesions can be distinguished by quantitative three-dimensional DOT. PMID:19405750

Eliminating Bias In Acousto-Optical Spectrum Analysis

NASA Technical Reports Server (NTRS)

Ansari, Homayoon; Lesh, James R.

1992-01-01

Scheme for digital processing of video signals in acousto-optical spectrum analyzer provides real-time correction for signal-dependent spectral bias. Spectrum analyzer described in "Two-Dimensional Acousto-Optical Spectrum Analyzer" (NPO-18092), related apparatus described in "Three-Dimensional Acousto-Optical Spectrum Analyzer" (NPO-18122). Essence of correction is to average over digitized outputs of pixels in each CCD row and to subtract this from the digitized output of each pixel in row. Signal processed electro-optically with reference-function signals to form two-dimensional spectral image in CCD camera.

Three-dimensional quantitative flow diagnostics

NASA Technical Reports Server (NTRS)

Miles, Richard B.; Nosenchuck, Daniel M.

1989-01-01

The principles, capabilities, and practical implementation of advanced measurement techniques for the quantitative characterization of three-dimensional flows are reviewed. Consideration is given to particle, Rayleigh, and Raman scattering; fluorescence; flow marking by H2 bubbles, photochromism, photodissociation, and vibrationally excited molecules; light-sheet volume imaging; and stereo imaging. Also discussed are stereo schlieren methods, holographic particle imaging, optical tomography, acoustic and magnetic-resonance imaging, and the display of space-filling data. Extensive diagrams, graphs, photographs, sample images, and tables of numerical data are provided.

Three-dimensional optical-transfer-function analysis of fiber-optical two-photon fluorescence microscopy.

PubMed

Gu, Min; Bird, Damian

2003-05-01

The three-dimensional optical transfer function is derived for analyzing the imaging performance in fiber-optical two-photon fluorescence microscopy. Two types of fiber-optical geometry are considered: The first involves a single-mode fiber for delivering a laser beam for illumination, and the second is based on the use of a single-mode fiber coupler for both illumination delivery and signal collection. It is found that in the former case the transverse and axial cutoff spatial frequencies of the three-dimensional optical transfer function are the same as those in conventional two-photon fluorescence microscopy without the use of a pinhole.However, the transverse and axial cutoff spatial frequencies in the latter case are 1.7 times as large as those in the former case. Accordingly, this feature leads to an enhanced optical sectioning effect when a fiber coupler is used, which is consistent with our recent experimental observation.

High-resolution x-ray guided three-dimensional diffuse optical tomography of joint tissues in hand osteoarthritis: Morphological and functional assessments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yuan Zhen; Zhang Qizhi; Sobel, Eric S.

Purpose: The aim of this study was to investigate the potential use of multimodality functional imaging techniques to identify the quantitative optical findings that can be used to distinguish between osteoarthritic and normal finger joints. Methods: Between 2006 and 2009, the distal interphalangeal finger joints from 40 female subjects including 22 patients and 18 healthy controls were examined clinically and scanned by a hybrid imaging system. This system integrated x-ray tomosynthetic setup with a diffuse optical imaging system. Optical absorption and scattering images were recovered based on a regularization-based hybrid reconstruction algorithm. A receiver operating characteristic curve was used tomore » calculate the statistical significance of specific optical features obtained from osteoarthritic and healthy joints groups. Results: The three-dimensional optical and x-ray images captured made it possible to quantify optical properties and joint space width of finger joints. Based on the recovered optical absorption and scattering parameters, the authors observed statistically significant differences between healthy and osteoarthritis finger joints. Conclusions: The statistical results revealed that sensitivity and specificity values up to 92% and 100%, respectively, can be achieved when optical properties of joint tissues were used as classifiers. This suggests that these optical imaging parameters are possible indicators for diagnosing osteoarthritis and monitoring its progression.« less

Three-dimensional fluorescent microscopy via simultaneous illumination and detection at multiple planes.

PubMed

Ma, Qian; Khademhosseinieh, Bahar; Huang, Eric; Qian, Haoliang; Bakowski, Malina A; Troemel, Emily R; Liu, Zhaowei

2016-08-16

The conventional optical microscope is an inherently two-dimensional (2D) imaging tool. The objective lens, eyepiece and image sensor are all designed to capture light emitted from a 2D 'object plane'. Existing technologies, such as confocal or light sheet fluorescence microscopy have to utilize mechanical scanning, a time-multiplexing process, to capture a 3D image. In this paper, we present a 3D optical microscopy method based upon simultaneously illuminating and detecting multiple focal planes. This is implemented by adding two diffractive optical elements to modify the illumination and detection optics. We demonstrate that the image quality of this technique is comparable to conventional light sheet fluorescent microscopy with the advantage of the simultaneous imaging of multiple axial planes and reduced number of scans required to image the whole sample volume.

Space imaging measurement system based on fixed lens and moving detector

NASA Astrophysics Data System (ADS)

Akiyama, Akira; Doshida, Minoru; Mutoh, Eiichiro; Kumagai, Hideo; Yamada, Hirofumi; Ishii, Hiromitsu

2006-08-01

We have developed the Space Imaging Measurement System based on the fixed lens and fast moving detector to the control of the autonomous ground vehicle. The space measurement is the most important task in the development of the autonomous ground vehicle. In this study we move the detector back and forth along the optical axis at the fast rate to measure the three-dimensional image data. This system is just appropriate to the autonomous ground vehicle because this system does not send out any optical energy to measure the distance and keep the safety. And we use the digital camera of the visible ray range. Therefore it gives us the cost reduction of the three-dimensional image data acquisition with respect to the imaging laser system. We can combine many pieces of the narrow space imaging measurement data to construct the wide range three-dimensional data. This gives us the improvement of the image recognition with respect to the object space. To develop the fast movement of the detector, we build the counter mass balance in the mechanical crank system of the Space Imaging Measurement System. And then we set up the duct to prevent the optical noise due to the ray not coming through lens. The object distance is derived from the focus distance which related to the best focused image data. The best focused image data is selected from the image of the maximum standard deviation in the standard deviations of series images.

Non-Contact Optical Ultrasound Concept for Biomedical Imaging

DTIC Science & Technology

2016-11-03

Non -Contact Optical Ultrasound Concept for Biomedical Imaging Robert Haupt1, Charles Wynn1, Jonathan Fincke2, Shawn Zhang2, Brian Anthony2...results. Lastly, we present imaging capabilities using a non -contact laser ultrasound proof-of-concept system. Two and three dimensional time... non -contact, standoff optical ultrasound has the potential to provide a fixed reference measurement capability that minimizes operator variability as

Implementation of a computationally efficient least-squares algorithm for highly under-determined three-dimensional diffuse optical tomography problems.

PubMed

Yalavarthy, Phaneendra K; Lynch, Daniel R; Pogue, Brian W; Dehghani, Hamid; Paulsen, Keith D

2008-05-01

Three-dimensional (3D) diffuse optical tomography is known to be a nonlinear, ill-posed and sometimes under-determined problem, where regularization is added to the minimization to allow convergence to a unique solution. In this work, a generalized least-squares (GLS) minimization method was implemented, which employs weight matrices for both data-model misfit and optical properties to include their variances and covariances, using a computationally efficient scheme. This allows inversion of a matrix that is of a dimension dictated by the number of measurements, instead of by the number of imaging parameters. This increases the computation speed up to four times per iteration in most of the under-determined 3D imaging problems. An analytic derivation, using the Sherman-Morrison-Woodbury identity, is shown for this efficient alternative form and it is proven to be equivalent, not only analytically, but also numerically. Equivalent alternative forms for other minimization methods, like Levenberg-Marquardt (LM) and Tikhonov, are also derived. Three-dimensional reconstruction results indicate that the poor recovery of quantitatively accurate values in 3D optical images can also be a characteristic of the reconstruction algorithm, along with the target size. Interestingly, usage of GLS reconstruction methods reduces error in the periphery of the image, as expected, and improves by 20% the ability to quantify local interior regions in terms of the recovered optical contrast, as compared to LM methods. Characterization of detector photo-multiplier tubes noise has enabled the use of the GLS method for reconstructing experimental data and showed a promise for better quantification of target in 3D optical imaging. Use of these new alternative forms becomes effective when the ratio of the number of imaging property parameters exceeds the number of measurements by a factor greater than 2.

Three-dimensional propagation in near-field tomographic X-ray phase retrieval

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ruhlandt, Aike, E-mail: aruhlan@gwdg.de; Salditt, Tim

An extension of phase retrieval algorithms for near-field X-ray (propagation) imaging to three dimensions is presented, enhancing the quality of the reconstruction by exploiting previously unused three-dimensional consistency constraints. This paper presents an extension of phase retrieval algorithms for near-field X-ray (propagation) imaging to three dimensions, enhancing the quality of the reconstruction by exploiting previously unused three-dimensional consistency constraints. The approach is based on a novel three-dimensional propagator and is derived for the case of optically weak objects. It can be easily implemented in current phase retrieval architectures, is computationally efficient and reduces the need for restrictive prior assumptions, resultingmore » in superior reconstruction quality.« less

Time-Resolved and Spectroscopic Three-Dimensional Optical Breast Tomography

DTIC Science & Technology

2008-04-01

Appendix 1. Each raw image was then cropped to select out the information-rich region, and binned to enhance the signal-to-noise ratio. All the binned...component analysis, near infrared (NIR) imaging, optical mammography , optical imaging using independent component analysis (OPTICA). I. INTRODUCTION N EAR...merging 5 × 5 pixels into one to enhance the SNR, resulting in a total of 352 images of 54 × 55 pixels each. All the binned images corresponding to

Three Dimensional Imaging of Cold Atoms in a Magneto Optical Trap with a Light Field Microscope

DTIC Science & Technology

2017-09-14

dimensional (3D) volume of the atoms is reconstructed using a modeled point spread function (PSF), taking into consideration the low magnification (1.25...axis fluorescence image. Optical axis separation between two atom clouds is measured to a 100µm accuracy in a 3mm deep volume , with a 16µm in-focus...79 vi Page 4.5 Phase Term Effects on the 3D Volume

High-resolution three-dimensional imaging of red blood cells parasitized by Plasmodium falciparum and in situ hemozoin crystals using optical diffraction tomography

NASA Astrophysics Data System (ADS)

Kim, Kyoohyun; Yoon, HyeOk; Diez-Silva, Monica; Dao, Ming; Dasari, Ramachandra R.; Park, YongKeun

2014-01-01

We present high-resolution optical tomographic images of human red blood cells (RBC) parasitized by malaria-inducing Plasmodium falciparum (Pf)-RBCs. Three-dimensional (3-D) refractive index (RI) tomograms are reconstructed by recourse to a diffraction algorithm from multiple two-dimensional holograms with various angles of illumination. These 3-D RI tomograms of Pf-RBCs show cellular and subcellular structures of host RBCs and invaded parasites in fine detail. Full asexual intraerythrocytic stages of parasite maturation (ring to trophozoite to schizont stages) are then systematically investigated using optical diffraction tomography algorithms. These analyses provide quantitative information on the structural and chemical characteristics of individual host Pf-RBCs, parasitophorous vacuole, and cytoplasm. The in situ structural evolution and chemical characteristics of subcellular hemozoin crystals are also elucidated.

High-resolution three-dimensional imaging of red blood cells parasitized by Plasmodium falciparum and in situ hemozoin crystals using optical diffraction tomography

PubMed Central

Kim, Kyoohyun; Yoon, HyeOk; Diez-Silva, Monica; Dao, Ming; Dasari, Ramachandra R.

2013-01-01

Abstract. We present high-resolution optical tomographic images of human red blood cells (RBC) parasitized by malaria-inducing Plasmodium falciparum (Pf)-RBCs. Three-dimensional (3-D) refractive index (RI) tomograms are reconstructed by recourse to a diffraction algorithm from multiple two-dimensional holograms with various angles of illumination. These 3-D RI tomograms of Pf-RBCs show cellular and subcellular structures of host RBCs and invaded parasites in fine detail. Full asexual intraerythrocytic stages of parasite maturation (ring to trophozoite to schizont stages) are then systematically investigated using optical diffraction tomography algorithms. These analyses provide quantitative information on the structural and chemical characteristics of individual host Pf-RBCs, parasitophorous vacuole, and cytoplasm. The in situ structural evolution and chemical characteristics of subcellular hemozoin crystals are also elucidated. PMID:23797986

Extracting cardiac shapes and motion of the chick embryo heart outflow tract from four-dimensional optical coherence tomography images

NASA Astrophysics Data System (ADS)

Yin, Xin; Liu, Aiping; Thornburg, Kent L.; Wang, Ruikang K.; Rugonyi, Sandra

2012-09-01

Recent advances in optical coherence tomography (OCT), and the development of image reconstruction algorithms, enabled four-dimensional (4-D) (three-dimensional imaging over time) imaging of the embryonic heart. To further analyze and quantify the dynamics of cardiac beating, segmentation procedures that can extract the shape of the heart and its motion are needed. Most previous studies analyzed cardiac image sequences using manually extracted shapes and measurements. However, this is time consuming and subject to inter-operator variability. Automated or semi-automated analyses of 4-D cardiac OCT images, although very desirable, are also extremely challenging. This work proposes a robust algorithm to semi automatically detect and track cardiac tissue layers from 4-D OCT images of early (tubular) embryonic hearts. Our algorithm uses a two-dimensional (2-D) deformable double-line model (DLM) to detect target cardiac tissues. The detection algorithm uses a maximum-likelihood estimator and was successfully applied to 4-D in vivo OCT images of the heart outflow tract of day three chicken embryos. The extracted shapes captured the dynamics of the chick embryonic heart outflow tract wall, enabling further analysis of cardiac motion.

Image formation of thick three-dimensional objects in differential-interference-contrast microscopy.

PubMed

Trattner, Sigal; Kashdan, Eugene; Feigin, Micha; Sochen, Nir

2014-05-01

The differential-interference-contrast (DIC) microscope is of widespread use in life sciences as it enables noninvasive visualization of transparent objects. The goal of this work is to model the image formation process of thick three-dimensional objects in DIC microscopy. The model is based on the principles of electromagnetic wave propagation and scattering. It simulates light propagation through the components of the DIC microscope to the image plane using a combined geometrical and physical optics approach and replicates the DIC image of the illuminated object. The model is evaluated by comparing simulated images of three-dimensional spherical objects with the recorded images of polystyrene microspheres. Our computer simulations confirm that the model captures the major DIC image characteristics of the simulated object, and it is sensitive to the defocusing effects.

Multi-particle three-dimensional coordinate estimation in real-time optical manipulation

NASA Astrophysics Data System (ADS)

Dam, J. S.; Perch-Nielsen, I.; Palima, D.; Gluckstad, J.

2009-11-01

We have previously shown how stereoscopic images can be obtained in our three-dimensional optical micromanipulation system [J. S. Dam et al, Opt. Express 16, 7244 (2008)]. Here, we present an extension and application of this principle to automatically gather the three-dimensional coordinates for all trapped particles with high tracking range and high reliability without requiring user calibration. Through deconvolving of the red, green, and blue colour planes to correct for bleeding between colour planes, we show that we can extend the system to also utilize green illumination, in addition to the blue and red. Applying the green colour as on-axis illumination yields redundant information for enhanced error correction, which is used to verify the gathered data, resulting in reliable coordinates as well as producing visually attractive images.

Wigner analysis of three dimensional pupil with finite lateral aperture

PubMed Central

Chen, Hsi-Hsun; Oh, Se Baek; Zhai, Xiaomin; Tsai, Jui-Chang; Cao, Liang-Cai; Barbastathis, George; Luo, Yuan

2015-01-01

A three dimensional (3D) pupil is an optical element, most commonly implemented on a volume hologram, that processes the incident optical field on a 3D fashion. Here we analyze the diffraction properties of a 3D pupil with finite lateral aperture in the 4-f imaging system configuration, using the Wigner Distribution Function (WDF) formulation. Since 3D imaging pupil is finite in both lateral and longitudinal directions, the WDF of the volume holographic 4-f imager theoretically predicts distinct Bragg diffraction patterns in phase space. These result in asymmetric profiles of diffracted coherent point spread function between degenerate diffraction and Bragg diffraction, elucidating the fundamental performance of volume holographic imaging. Experimental measurements are also presented, confirming the theoretical predictions. PMID:25836443

Longitudinally Jointed Edge-Wise Compression HoneyComb Composite Sandwich Coupon Testing And Fe Analysis: Three Methods of Strain Measurement, And Comparison

NASA Technical Reports Server (NTRS)

Farrokh, Babak; Rahim, Nur Aida Abul; Segal, Ken; Fan, Terry; Jones, Justin; Hodges, Ken; Mashni, Noah; Garg, Naman; Sang, Alex

2013-01-01

Three distinct strain measurement methods (i.e., foil resistance strain gages, fiber optic strain sensors, and a three-dimensional digital image photogrammetry that gives full field strain and displacement measurements) were implemented to measure strains on the back and front surfaces of a longitudinally jointed curved test article subjected to edge-wise compression testing, at NASA Goddard Space Flight Center, according to ASTM C364. The pre-test finite element analysis (FEA) was conducted to assess ultimate failure load and predict strain distribution pattern throughout the test coupon. The predicted strain pattern contours were then utilized as guidelines for installing the strain measurement instrumentations. The foil resistance strain gages and fiber optic strain sensors were bonded on the specimen at locations with nearly the same analytically predicted strain values, and as close as possible to each other, so that, comparisons between the measured strains by strain gages and fiber optic sensors, as well as the three-dimensional digital image photogrammetric system are relevant. The test article was loaded to failure (at 167 kN), at the compressive strain value of 10,000 micro epsilon. As a part of this study, the validity of the measured strains by fiber optic sensors is examined against the foil resistance strain gages and the three-dimensional digital image photogrammetric data, and comprehensive comparisons are made with FEA predictions.

Three-dimensional image display system using stereogram and holographic optical memory techniques

NASA Astrophysics Data System (ADS)

Kim, Cheol S.; Kim, Jung G.; Shin, Chang-Mok; Kim, Soo-Joong

2001-09-01

In this paper, we implemented a three dimensional image display system using stereogram and holographic optical memory techniques which can store many images and reconstruct them automatically. In this system, to store and reconstruct stereo images, incident angle of reference beam must be controlled in real time, so we used BPH (binary phase hologram) and LCD (liquid crystal display) for controlling reference beam. And input images are represented on the LCD without polarizer/analyzer for maintaining uniform beam intensities regardless of the brightness of input images. The input images and BPHs are edited using application software with having the same recording scheduled time interval in storing. The reconstructed stereo images are acquired by capturing the output images with CCD camera at the behind of the analyzer which transforms phase information into brightness information of images. The reference beams are acquired by Fourier transform of BPH which designed with SA (simulated annealing) algorithm, and represented on the LCD with the 0.05 seconds time interval using application software for reconstructing the stereo images. In output plane, we used a LCD shutter that is synchronized to a monitor that displays alternate left and right eye images for depth perception. We demonstrated optical experiment which store and reconstruct four stereo images in BaTiO3 repeatedly using holographic optical memory techniques.

Volume three-dimensional flow measurements using wavelength multiplexing.

PubMed

Moore, Andrew J; Smith, Jason; Lawson, Nicholas J

2005-10-01

Optically distinguishable seeding particles that emit light in a narrow bandwidth, and a combination of bandwidths, were prepared by encapsulating quantum dots. The three-dimensional components of the particles' displacement were measured within a volume of fluid with particle tracking velocimetry (PTV). Particles are multiplexed to different hue bands in the camera images, enabling an increased seeding density and (or) fewer cameras to be used, thereby increasing the measurement spatial resolution and (or) reducing optical access requirements. The technique is also applicable to two-phase flow measurements with PTV or particle image velocimetry, where each phase is uniquely seeded.

Three-dimensional vascular imaging of proliferative diabetic retinopathy by Doppler optical coherence tomography.

PubMed

Miura, Masahiro; Hong, Young-Joo; Yasuno, Yoshiaki; Muramatsu, Daisuke; Iwasaki, Takuya; Goto, Hiroshi

2015-03-01

To evaluate the 3-dimensional architecture of neovascularization in proliferative diabetic retinopathy using Doppler optical coherence tomography (OCT). Prospective, nonrandomized clinical trial. Seventeen eyes of 14 patients with proliferative diabetic retinopathy were prospectively studied. Prototype Doppler OCT was used to evaluate the 3-dimensional vascular architecture at vitreoretinal adhesions. Proliferative membranes were detected in all eyes with proliferative diabetic retinopathy by standard OCT images. Doppler OCT images detected blood flow by neovascularization of the disc in 12 eyes and neovascularization elsewhere in 11 eyes. Doppler OCT images showed the 3-dimensional extent of new vessels at various stages of neovascularization, and the extent of new vessels could be clearly confirmed at vitreoretinal adhesions. Doppler OCT is useful for the detection and evaluation of the 3-dimensional vascular structure of neovascularization, and can assist in the noninvasive assessment of proliferative diabetic retinopathy. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells

PubMed Central

Rodriguez, Jose A.; Xu, Rui; Chen, Chien-Chun; Huang, Zhifeng; Jiang, Huaidong; Chen, Allan L.; Raines, Kevin S.; Pryor Jr, Alan; Nam, Daewoong; Wiegart, Lutz; Song, Changyong; Madsen, Anders; Chushkin, Yuriy; Zontone, Federico; Bradley, Peter J.; Miao, Jianwei

2015-01-01

A structural understanding of whole cells in three dimensions at high spatial resolution remains a significant challenge and, in the case of X-rays, has been limited by radiation damage. By alleviating this limitation, cryogenic coherent diffractive imaging (cryo-CDI) can in principle be used to bridge the important resolution gap between optical and electron microscopy in bio-imaging. Here, the first experimental demonstration of cryo-CDI for quantitative three-dimensional imaging of whole frozen-hydrated cells using 8 keV X-rays is reported. As a proof of principle, a tilt series of 72 diffraction patterns was collected from a frozen-hydrated Neospora caninum cell and the three-dimensional mass density of the cell was reconstructed and quantified based on its natural contrast. This three-dimensional reconstruction reveals the surface and internal morphology of the cell, including its complex polarized sub-cellular structure. It is believed that this work represents an experimental milestone towards routine quantitative three-dimensional imaging of whole cells in their natural state with spatial resolutions in the tens of nanometres. PMID:26306199

Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells

DOE PAGES

Rodriguez, Jose A.; Xu, Rui; Chen, Chien -Chun; ...

2015-09-01

Here, a structural understanding of whole cells in three dimensions at high spatial resolution remains a significant challenge and, in the case of X-rays, has been limited by radiation damage. By alleviating this limitation, cryogenic coherent diffractive imaging (cryo-CDI) can in principle be used to bridge the important resolution gap between optical and electron microscopy in bio-imaging. Here, the first experimental demonstration of cryo-CDI for quantitative three-dimensional imaging of whole frozen-hydrated cells using 8 Kev X-rays is reported. As a proof of principle, a tilt series of 72 diffraction patterns was collected from a frozen-hydrated Neospora caninum cell and themore » three-dimensional mass density of the cell was reconstructed and quantified based on its natural contrast. This three-dimensional reconstruction reveals the surface and internal morphology of the cell, including its complex polarized sub-cellular structure. Finally, it is believed that this work represents an experimental milestone towards routine quantitative three-dimensional imaging of whole cells in their natural state with spatial resolutions in the tens of nanometres.« less

Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells.

PubMed

Rodriguez, Jose A; Xu, Rui; Chen, Chien-Chun; Huang, Zhifeng; Jiang, Huaidong; Chen, Allan L; Raines, Kevin S; Pryor, Alan; Nam, Daewoong; Wiegart, Lutz; Song, Changyong; Madsen, Anders; Chushkin, Yuriy; Zontone, Federico; Bradley, Peter J; Miao, Jianwei

2015-09-01

A structural understanding of whole cells in three dimensions at high spatial resolution remains a significant challenge and, in the case of X-rays, has been limited by radiation damage. By alleviating this limitation, cryogenic coherent diffractive imaging (cryo-CDI) can in principle be used to bridge the important resolution gap between optical and electron microscopy in bio-imaging. Here, the first experimental demonstration of cryo-CDI for quantitative three-dimensional imaging of whole frozen-hydrated cells using 8 keV X-rays is reported. As a proof of principle, a tilt series of 72 diffraction patterns was collected from a frozen-hydrated Neospora caninum cell and the three-dimensional mass density of the cell was reconstructed and quantified based on its natural contrast. This three-dimensional reconstruction reveals the surface and internal morphology of the cell, including its complex polarized sub-cellular structure. It is believed that this work represents an experimental milestone towards routine quantitative three-dimensional imaging of whole cells in their natural state with spatial resolutions in the tens of nanometres.

Fiber bundle endomicroscopy with multi-illumination for three-dimensional reflectance image reconstruction

NASA Astrophysics Data System (ADS)

Ando, Yoriko; Sawahata, Hirohito; Kawano, Takeshi; Koida, Kowa; Numano, Rika

2018-02-01

Bundled fiber optics allow in vivo imaging at deep sites in a body. The intrinsic optical contrast detects detailed structures in blood vessels and organs. We developed a bundled-fiber-coupled endomicroscope, enabling stereoscopic three-dimensional (3-D) reflectance imaging with a multipositional illumination scheme. Two illumination sites were attached to obtain reflectance images with left and right illumination. Depth was estimated by the horizontal disparity between the two images under alternative illuminations and was calibrated by the targets with known depths. This depth reconstruction was applied to an animal model to obtain the 3-D structure of blood vessels of the cerebral cortex (Cereb cortex) and preputial gland (Pre gla). The 3-D endomicroscope could be instrumental to microlevel reflectance imaging, improving the precision in subjective depth perception, spatial orientation, and identification of anatomical structures.

A photophoretic-trap volumetric display

NASA Astrophysics Data System (ADS)

Smalley, D. E.; Nygaard, E.; Squire, K.; van Wagoner, J.; Rasmussen, J.; Gneiting, S.; Qaderi, K.; Goodsell, J.; Rogers, W.; Lindsey, M.; Costner, K.; Monk, A.; Pearson, M.; Haymore, B.; Peatross, J.

2018-01-01

Free-space volumetric displays, or displays that create luminous image points in space, are the technology that most closely resembles the three-dimensional displays of popular fiction. Such displays are capable of producing images in ‘thin air’ that are visible from almost any direction and are not subject to clipping. Clipping restricts the utility of all three-dimensional displays that modulate light at a two-dimensional surface with an edge boundary; these include holographic displays, nanophotonic arrays, plasmonic displays, lenticular or lenslet displays and all technologies in which the light scattering surface and the image point are physically separate. Here we present a free-space volumetric display based on photophoretic optical trapping that produces full-colour graphics in free space with ten-micrometre image points using persistence of vision. This display works by first isolating a cellulose particle in a photophoretic trap created by spherical and astigmatic aberrations. The trap and particle are then scanned through a display volume while being illuminated with red, green and blue light. The result is a three-dimensional image in free space with a large colour gamut, fine detail and low apparent speckle. This platform, named the Optical Trap Display, is capable of producing image geometries that are currently unobtainable with holographic and light-field technologies, such as long-throw projections, tall sandtables and ‘wrap-around’ displays.

Molecular imaging needles: dual-modality optical coherence tomography and fluorescence imaging of labeled antibodies deep in tissue

PubMed Central

Scolaro, Loretta; Lorenser, Dirk; Madore, Wendy-Julie; Kirk, Rodney W.; Kramer, Anne S.; Yeoh, George C.; Godbout, Nicolas; Sampson, David D.; Boudoux, Caroline; McLaughlin, Robert A.

2015-01-01

Molecular imaging using optical techniques provides insight into disease at the cellular level. In this paper, we report on a novel dual-modality probe capable of performing molecular imaging by combining simultaneous three-dimensional optical coherence tomography (OCT) and two-dimensional fluorescence imaging in a hypodermic needle. The probe, referred to as a molecular imaging (MI) needle, may be inserted tens of millimeters into tissue. The MI needle utilizes double-clad fiber to carry both imaging modalities, and is interfaced to a 1310-nm OCT system and a fluorescence imaging subsystem using an asymmetrical double-clad fiber coupler customized to achieve high fluorescence collection efficiency. We present, to the best of our knowledge, the first dual-modality OCT and fluorescence needle probe with sufficient sensitivity to image fluorescently labeled antibodies. Such probes enable high-resolution molecular imaging deep within tissue. PMID:26137379

Measurements of morphology and refractive indexes on human downy hairs using three-dimensional quantitative phase imaging.

PubMed

Lee, SangYun; Kim, Kyoohyun; Lee, Yuhyun; Park, Sungjin; Shin, Heejae; Yang, Jongwon; Ko, Kwanhong; Park, HyunJoo; Park, YongKeun

2015-01-01

We present optical measurements of morphology and refractive indexes (RIs) of human downy arm hairs using three-dimensional (3-D) quantitative phase imaging techniques. 3-D RI tomograms and high-resolution two-dimensional synthetic aperture images of individual downy arm hairs were measured using a Mach–Zehnder laser interferometric microscopy equipped with a two-axis galvanometer mirror. From the measured quantitative images, the RIs and morphological parameters of downy hairs were noninvasively quantified including the mean RI, volume, cylinder, and effective radius of individual hairs. In addition, the effects of hydrogen peroxide on individual downy hairs were investigated.

Three-dimensional Optical Coherence Tomography Imaging and Treatment of Glaucomatous Optic Nerve Head Defects Associated with Schisis-like Maculopathy

PubMed Central

Öztaş, Zafer; Menteş, Jale; Ateş, Halil; Nalçacı, Serhad

2017-01-01

We present the three-dimensional (3D) spectral-domain optical coherence tomography (SD-OCT) findings of schisis-like maculopathy associated with structural changes of the optic nerve (ON) head as well as the treatment outcomes of a case of advanced glaucoma. In addition to ophthalmological examination, B-scan and 3D-SD-OCT images of the ON head, peripapillary retina, and the macula were obtained. The B-scan images only detected typical retinoschisis findings. However, the 3D-SD-OCT images of the ON head revealed defects of various sizes, shapes, and depths at the outer wall of the prelaminar and laminar regions of the ON canal. The 3D images were able to establish that these defects were both adjacent to and interconnected with the retinal layers. The patient successfully received 3D-SD-OCT-guided thermal laser treatment that is used in congenital optic disc pits complicated with macular schisis. In brief, 3D-SD-OCT is very useful for demonstrating the ON head defects that can lead to schisis-like maculopathy in cases of advanced glaucoma. PMID:28405489

Automatic classification of retinal three-dimensional optical coherence tomography images using principal component analysis network with composite kernels.

PubMed

Fang, Leyuan; Wang, Chong; Li, Shutao; Yan, Jun; Chen, Xiangdong; Rabbani, Hossein

2017-11-01

We present an automatic method, termed as the principal component analysis network with composite kernel (PCANet-CK), for the classification of three-dimensional (3-D) retinal optical coherence tomography (OCT) images. Specifically, the proposed PCANet-CK method first utilizes the PCANet to automatically learn features from each B-scan of the 3-D retinal OCT images. Then, multiple kernels are separately applied to a set of very important features of the B-scans and these kernels are fused together, which can jointly exploit the correlations among features of the 3-D OCT images. Finally, the fused (composite) kernel is incorporated into an extreme learning machine for the OCT image classification. We tested our proposed algorithm on two real 3-D spectral domain OCT (SD-OCT) datasets (of normal subjects and subjects with the macular edema and age-related macular degeneration), which demonstrated its effectiveness. (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

Photoacoustic projection imaging using an all-optical detector array

NASA Astrophysics Data System (ADS)

Bauer-Marschallinger, J.; Felbermayer, K.; Berer, T.

2018-02-01

We present a prototype for all-optical photoacoustic projection imaging. By generating projection images, photoacoustic information of large volumes can be retrieved with less effort compared to common photoacoustic computed tomography where many detectors and/or multiple measurements are required. In our approach, an array of 60 integrating line detectors is used to acquire photoacoustic waves. The line detector array consists of fiber-optic MachZehnder interferometers, distributed on a cylindrical surface. From the measured variation of the optical path lengths of the interferometers, induced by photoacoustic waves, a photoacoustic projection image can be reconstructed. The resulting images represent the projection of the three-dimensional spatial light absorbance within the imaged object onto a two-dimensional plane, perpendicular to the line detector array. The fiber-optic detectors achieve a noise-equivalent pressure of 24 Pascal at a 10 MHz bandwidth. We present the operational principle, the structure of the array, and resulting images. The system can acquire high-resolution projection images of large volumes within a short period of time. Imaging large volumes at high frame rates facilitates monitoring of dynamic processes.

Three-dimensional fluorescence-enhanced optical tomography using a hand-held probe based imaging system

PubMed Central

Ge, Jiajia; Zhu, Banghe; Regalado, Steven; Godavarty, Anuradha

2008-01-01

Hand-held based optical imaging systems are a recent development towards diagnostic imaging of breast cancer. To date, all the hand-held based optical imagers are used to perform only surface mapping and target localization, but are not capable of demonstrating tomographic imaging. Herein, a novel hand-held probe based optical imager is developed towards three-dimensional (3-D) optical tomography studies. The unique features of this optical imager, which primarily consists of a hand-held probe and an intensified charge coupled device detector, are its ability to; (i) image large tissue areas (5×10 sq. cm) in a single scan, (ii) perform simultaneous multiple point illumination and collection, thus reducing the overall imaging time; and (iii) adapt to varying tissue curvatures, from a flexible probe head design. Experimental studies are performed in the frequency domain on large slab phantoms (∼650 ml) using fluorescence target(s) under perfect uptake (1:0) contrast ratios, and varying target depths (1–2 cm) and X-Y locations. The effect of implementing simultaneous over sequential multiple point illumination towards 3-D tomography is experimentally demonstrated. The feasibility of 3-D optical tomography studies has been demonstrated for the first time using a hand-held based optical imager. Preliminary fluorescence-enhanced optical tomography studies are able to reconstruct 0.45 ml target(s) located at different target depths (1–2 cm). However, the depth recovery was limited as the actual target depth increased, since only reflectance measurements were acquired. Extensive tomography studies are currently carried out to determine the resolution and performance limits of the imager on flat and curved phantoms. PMID:18697559

Three-dimensional fluorescence-enhanced optical tomography using a hand-held probe based imaging system.

PubMed

Ge, Jiajia; Zhu, Banghe; Regalado, Steven; Godavarty, Anuradha

2008-07-01

Hand-held based optical imaging systems are a recent development towards diagnostic imaging of breast cancer. To date, all the hand-held based optical imagers are used to perform only surface mapping and target localization, but are not capable of demonstrating tomographic imaging. Herein, a novel hand-held probe based optical imager is developed towards three-dimensional (3-D) optical tomography studies. The unique features of this optical imager, which primarily consists of a hand-held probe and an intensified charge coupled device detector, are its ability to; (i) image large tissue areas (5 x 10 sq. cm) in a single scan, (ii) perform simultaneous multiple point illumination and collection, thus reducing the overall imaging time; and (iii) adapt to varying tissue curvatures, from a flexible probe head design. Experimental studies are performed in the frequency domain on large slab phantoms (approximately 650 ml) using fluorescence target(s) under perfect uptake (1:0) contrast ratios, and varying target depths (1-2 cm) and X-Y locations. The effect of implementing simultaneous over sequential multiple point illumination towards 3-D tomography is experimentally demonstrated. The feasibility of 3-D optical tomography studies has been demonstrated for the first time using a hand-held based optical imager. Preliminary fluorescence-enhanced optical tomography studies are able to reconstruct 0.45 ml target(s) located at different target depths (1-2 cm). However, the depth recovery was limited as the actual target depth increased, since only reflectance measurements were acquired. Extensive tomography studies are currently carried out to determine the resolution and performance limits of the imager on flat and curved phantoms.

Multifunctional, three-dimensional tomography for analysis of eletrectrohydrodynamic jetting

NASA Astrophysics Data System (ADS)

Nguyen, Xuan Hung; Gim, Yeonghyeon; Ko, Han Seo

2015-05-01

A three-dimensional optical tomography technique was developed to reconstruct three-dimensional objects using a set of two-dimensional shadowgraphic images and normal gray images. From three high-speed cameras, which were positioned at an offset angle of 45° between each other, number, size, and location of electrohydrodynamic jets with respect to the nozzle position were analyzed using shadowgraphic tomography employing multiplicative algebraic reconstruction technique (MART). Additionally, a flow field inside a cone-shaped liquid (Taylor cone) induced under an electric field was observed using a simultaneous multiplicative algebraic reconstruction technique (SMART), a tomographic method for reconstructing light intensities of particles, combined with three-dimensional cross-correlation. Various velocity fields of circulating flows inside the cone-shaped liquid caused by various physico-chemical properties of liquid were also investigated.

Three-dimensional nanoscale imaging by plasmonic Brownian microscopy

NASA Astrophysics Data System (ADS)

Labno, Anna; Gladden, Christopher; Kim, Jeongmin; Lu, Dylan; Yin, Xiaobo; Wang, Yuan; Liu, Zhaowei; Zhang, Xiang

2017-12-01

Three-dimensional (3D) imaging at the nanoscale is a key to understanding of nanomaterials and complex systems. While scanning probe microscopy (SPM) has been the workhorse of nanoscale metrology, its slow scanning speed by a single probe tip can limit the application of SPM to wide-field imaging of 3D complex nanostructures. Both electron microscopy and optical tomography allow 3D imaging, but are limited to the use in vacuum environment due to electron scattering and to optical resolution in micron scales, respectively. Here we demonstrate plasmonic Brownian microscopy (PBM) as a way to improve the imaging speed of SPM. Unlike photonic force microscopy where a single trapped particle is used for a serial scanning, PBM utilizes a massive number of plasmonic nanoparticles (NPs) under Brownian diffusion in solution to scan in parallel around the unlabeled sample object. The motion of NPs under an evanescent field is three-dimensionally localized to reconstruct the super-resolution topology of 3D dielectric objects. Our method allows high throughput imaging of complex 3D structures over a large field of view, even with internal structures such as cavities that cannot be accessed by conventional mechanical tips in SPM.

Ultrahigh-Speed Optical Coherence Tomography for Three-Dimensional and En Face Imaging of the Retina and Optic Nerve Head

PubMed Central

Srinivasan, Vivek J.; Adler, Desmond C.; Chen, Yueli; Gorczynska, Iwona; Huber, Robert; Duker, Jay S.; Schuman, Joel S.; Fujimoto, James G.

2009-01-01

Purpose To demonstrate ultrahigh-speed optical coherence tomography (OCT) imaging of the retina and optic nerve head at 249,000 axial scans per second and a wavelength of 1060 nm. To investigate methods for visualization of the retina, choroid, and optic nerve using high-density sampling enabled by improved imaging speed. Methods A swept-source OCT retinal imaging system operating at a speed of 249,000 axial scans per second was developed. Imaging of the retina, choroid, and optic nerve were performed. Display methods such as speckle reduction, slicing along arbitrary planes, en face visualization of reflectance from specific retinal layers, and image compounding were investigated. Results High-definition and three-dimensional (3D) imaging of the normal retina and optic nerve head were performed. Increased light penetration at 1060 nm enabled improved visualization of the choroid, lamina cribrosa, and sclera. OCT fundus images and 3D visualizations were generated with higher pixel density and less motion artifacts than standard spectral/Fourier domain OCT. En face images enabled visualization of the porous structure of the lamina cribrosa, nerve fiber layer, choroid, photoreceptors, RPE, and capillaries of the inner retina. Conclusions Ultrahigh-speed OCT imaging of the retina and optic nerve head at 249,000 axial scans per second is possible. The improvement of ∼5 to 10× in imaging speed over commercial spectral/Fourier domain OCT technology enables higher density raster scan protocols and improved performance of en face visualization methods. The combination of the longer wavelength and ultrahigh imaging speed enables excellent visualization of the choroid, sclera, and lamina cribrosa. PMID:18658089

Neuroanatomical phenotyping of the mouse brain with three-dimensional autofluorescence imaging

PubMed Central

Wong, Michael D.; Dazai, Jun; Altaf, Maliha; Mark Henkelman, R.; Lerch, Jason P.; Nieman, Brian J.

2012-01-01

The structural organization of the brain is important for normal brain function and is critical to understand in order to evaluate changes that occur during disease processes. Three-dimensional (3D) imaging of the mouse brain is necessary to appreciate the spatial context of structures within the brain. In addition, the small scale of many brain structures necessitates resolution at the ∼10 μm scale. 3D optical imaging techniques, such as optical projection tomography (OPT), have the ability to image intact large specimens (1 cm3) with ∼5 μm resolution. In this work we assessed the potential of autofluorescence optical imaging methods, and specifically OPT, for phenotyping the mouse brain. We found that both specimen size and fixation methods affected the quality of the OPT image. Based on these findings we developed a specimen preparation method to improve the images. Using this method we assessed the potential of optical imaging for phenotyping. Phenotypic differences between wild-type male and female mice were quantified using computer-automated methods. We found that optical imaging of the endogenous autofluorescence in the mouse brain allows for 3D characterization of neuroanatomy and detailed analysis of brain phenotypes. This will be a powerful tool for understanding mouse models of disease and development and is a technology that fits easily within the workflow of biology and neuroscience labs. PMID:22718750

Nanometric holograms based on a topological insulator material.

PubMed

Yue, Zengji; Xue, Gaolei; Liu, Juan; Wang, Yongtian; Gu, Min

2017-05-18

Holography has extremely extensive applications in conventional optical instruments spanning optical microscopy and imaging, three-dimensional displays and metrology. To integrate holography with modern low-dimensional electronic devices, holograms need to be thinned to a nanometric scale. However, to keep a pronounced phase shift modulation, the thickness of holograms has been generally limited to the optical wavelength scale, which hinders their integration with ultrathin electronic devices. Here, we break this limit and achieve 60 nm holograms using a topological insulator material. We discover that nanometric topological insulator thin films act as an intrinsic optical resonant cavity due to the unequal refractive indices in their metallic surfaces and bulk. The resonant cavity leads to enhancement of phase shifts and thus the holographic imaging. Our work paves a way towards integrating holography with flat electronic devices for optical imaging, data storage and information security.

Three-dimensional digital mapping of the optic nerve head cupping in glaucoma

NASA Astrophysics Data System (ADS)

Mitra, Sunanda; Ramirez, Manuel; Morales, Jose

1992-08-01

Visualization of the optic nerve head cupping is clinically achieved by stereoscopic viewing of a fundus image pair of the suspected eye. A novel algorithm for three-dimensional digital surface representation of the optic nerve head, using fusion of stereo depth map with a linearly stretched intensity image of a stereo fundus image pair, is presented. Prior to depth map acquisition, a number of preprocessing tasks including feature extraction, registration by cepstral analysis, and correction for intensity variations are performed. The depth map is obtained by using a coarse to fine strategy for obtaining disparities between corresponding areas. The required matching techniques to obtain the translational differences in every step, uses cepstral analysis and correlation-like scanning technique in the spatial domain for the finest details. The quantitative and precise representation of the optic nerve head surface topography following this algorithm is not computationally intensive and should provide more useful information than just qualitative stereoscopic viewing of the fundus as one of the diagnostic criteria for diagnosis of glaucoma.

Fast segmentation and high-quality three-dimensional volume mesh creation from medical images for diffuse optical tomography

NASA Astrophysics Data System (ADS)

Jermyn, Michael; Ghadyani, Hamid; Mastanduno, Michael A.; Turner, Wes; Davis, Scott C.; Dehghani, Hamid; Pogue, Brian W.

2013-08-01

Multimodal approaches that combine near-infrared (NIR) and conventional imaging modalities have been shown to improve optical parameter estimation dramatically and thus represent a prevailing trend in NIR imaging. These approaches typically involve applying anatomical templates from magnetic resonance imaging/computed tomography/ultrasound images to guide the recovery of optical parameters. However, merging these data sets using current technology requires multiple software packages, substantial expertise, significant time-commitment, and often results in unacceptably poor mesh quality for optical image reconstruction, a reality that represents a significant roadblock for translational research of multimodal NIR imaging. This work addresses these challenges directly by introducing automated digital imaging and communications in medicine image stack segmentation and a new one-click three-dimensional mesh generator optimized for multimodal NIR imaging, and combining these capabilities into a single software package (available for free download) with a streamlined workflow. Image processing time and mesh quality benchmarks were examined for four common multimodal NIR use-cases (breast, brain, pancreas, and small animal) and were compared to a commercial image processing package. Applying these tools resulted in a fivefold decrease in image processing time and 62% improvement in minimum mesh quality, in the absence of extra mesh postprocessing. These capabilities represent a significant step toward enabling translational multimodal NIR research for both expert and nonexpert users in an open-source platform.

Three-dimensional imaging through turbid media based on polarization-difference liquid-crystal microlens array

NASA Astrophysics Data System (ADS)

Xin, Zhaowei; Wei, Dong; Li, Dapeng; Xie, Xingwang; Chen, Mingce; Zhang, Xinyu; Wang, Haiwei; Xie, Changsheng

2018-02-01

In this paper, a polarization difference liquid-crystal microlens array (PD-LCMLA) for three dimensional imaging application through turbid media is fabricated and demonstrated. This device is composed of a twisted nematic liquidcrystal cell (TNLCC), a polarizer and a liquid-crystal microlens array. The polarizer is sandwiched between the TNLCC and LCMLA to help the polarization difference system achieving the orthogonal polarization raw images. The prototyped camera for polarization difference imaging has been constructed by integrating the PD-LCMLA with an image sensor. The orthogonally polarized light-field images are recorded by switching the working state of the TNLCC. Here, by using a special microstructure in conjunction with the polarization-difference algorithm, we demonstrate that the three-dimensional information in the scattering media can be retrieved from the polarization-difference imaging system with an electrically tunable PD-LCMLA. We further investigate the system's potential function based on the flexible microstructure. The microstructure provides a wide operation range in the manipulation of incident beams and also emerges multiple operation modes for imaging applications, such as conventional planar imaging, polarization imaging mode, and polarization-difference imaging mode. Since the PD-LCMLA demonstrates a very low power consumption, multiple imaging modes and simple manufacturing, this kind of device presents a potential to be used in many other optical and electro-optical systems.

Cross-talk reduction by correcting the subpixel position in a multiview autostereoscopic three-dimensional display based on a lenticular sheet.

PubMed

Wang, Qiong-Hua; Li, Xiao-Fang; Zhou, Lei; Wang, Ai-Hong; Li, Da-Hai

2011-03-01

A method is proposed to alleviate the cross talk in multiview autostereoscopic three-dimensional displays based on a lenticular sheet. We analyze the positional relationship between subpixels on the image panel and the lenticular sheet. According to this relationship, optimal synthetic images are synthesized to minimize cross talk by correcting the positions of subpixels on the image panel. Experimental results show that the proposed method significantly reduces the cross talk of view images and improves the quality of stereoscopic images. © 2010 Optical Society of America

Optical design and development of a snapshot light-field laryngoscope

NASA Astrophysics Data System (ADS)

Zhu, Shuaishuai; Jin, Peng; Liang, Rongguang; Gao, Liang

2018-02-01

The convergence of recent advances in optical fabrication and digital processing yields a generation of imaging technology-light-field (LF) cameras which bridge the realms of applied mathematics, optics, and high-performance computing. Herein for the first time, we introduce the paradigm of LF imaging into laryngoscopy. The resultant probe can image the three-dimensional shape of vocal folds within a single camera exposure. Furthermore, to improve the spatial resolution, we developed an image fusion algorithm, providing a simple solution to a long-standing problem in LF imaging.

Three-dimensional motion-picture imaging of dynamic object by parallel-phase-shifting digital holographic microscopy using an inverted magnification optical system

NASA Astrophysics Data System (ADS)

Fukuda, Takahito; Shinomura, Masato; Xia, Peng; Awatsuji, Yasuhiro; Nishio, Kenzo; Matoba, Osamu

2017-04-01

We constructed a parallel-phase-shifting digital holographic microscopy (PPSDHM) system using an inverted magnification optical system, and succeeded in three-dimensional (3D) motion-picture imaging for 3D displacement of a microscopic object. In the PPSDHM system, the inverted and afocal magnification optical system consisted of a microscope objective (16.56 mm focal length and 0.25 numerical aperture) and a convex lens (300 mm focal length and 82 mm aperture diameter). A polarization-imaging camera was used to record multiple phase-shifted holograms with a single-shot exposure. We recorded an alum crystal, sinking down in aqueous solution of alum, by the constructed PPSDHM system at 60 frames/s for about 20 s and reconstructed high-quality 3D motion-picture image of the crystal. Then, we calculated amounts of displacement of the crystal from the amounts in the focus plane and the magnifications of the magnification optical system, and obtained the 3D trajectory of the crystal by that amounts.

Introduction

NASA Astrophysics Data System (ADS)

Bouma, Brett E.

1998-09-01

The pace of technological advancement of Optical Coherence Tomography (OCT) over the last several years has been extremely rapid. The field has progressed from one-dimensional low-coherence ranging to full three-dimensional imaging with individual two-dimensional images aquired at near video rate in a span of less than eight years. Imaging applications have included polymers and advanced composites, Ophthalmology, Developmental Biology, Gastroenterology, Urology, Cardiology, Neurology, and Gynecology. These preliminary studies indicate the great potential for OCT to make a significant impact, especially in clinical medicine.

Accurate facade feature extraction method for buildings from three-dimensional point cloud data considering structural information

NASA Astrophysics Data System (ADS)

Wang, Yongzhi; Ma, Yuqing; Zhu, A.-xing; Zhao, Hui; Liao, Lixia

2018-05-01

Facade features represent segmentations of building surfaces and can serve as a building framework. Extracting facade features from three-dimensional (3D) point cloud data (3D PCD) is an efficient method for 3D building modeling. By combining the advantages of 3D PCD and two-dimensional optical images, this study describes the creation of a highly accurate building facade feature extraction method from 3D PCD with a focus on structural information. The new extraction method involves three major steps: image feature extraction, exploration of the mapping method between the image features and 3D PCD, and optimization of the initial 3D PCD facade features considering structural information. Results show that the new method can extract the 3D PCD facade features of buildings more accurately and continuously. The new method is validated using a case study. In addition, the effectiveness of the new method is demonstrated by comparing it with the range image-extraction method and the optical image-extraction method in the absence of structural information. The 3D PCD facade features extracted by the new method can be applied in many fields, such as 3D building modeling and building information modeling.

Laboratory-size three-dimensional x-ray microscope with Wolter type I mirror optics and an electron-impact water window x-ray source

NASA Astrophysics Data System (ADS)

Ohsuka, Shinji; Ohba, Akira; Onoda, Shinobu; Nakamoto, Katsuhiro; Nakano, Tomoyasu; Miyoshi, Motosuke; Soda, Keita; Hamakubo, Takao

2014-09-01

We constructed a laboratory-size three-dimensional water window x-ray microscope that combines wide-field transmission x-ray microscopy with tomographic reconstruction techniques, and observed bio-medical samples to evaluate its applicability to life science research fields. It consists of a condenser and an objective grazing incidence Wolter type I mirror, an electron-impact type oxygen Kα x-ray source, and a back-illuminated CCD for x-ray imaging. A spatial resolution limit of around 1.0 line pairs per micrometer was obtained for two-dimensional transmission images, and 1-μm scale three-dimensional fine structures were resolved.

Laboratory-size three-dimensional x-ray microscope with Wolter type I mirror optics and an electron-impact water window x-ray source.

PubMed

Ohsuka, Shinji; Ohba, Akira; Onoda, Shinobu; Nakamoto, Katsuhiro; Nakano, Tomoyasu; Miyoshi, Motosuke; Soda, Keita; Hamakubo, Takao

2014-09-01

We constructed a laboratory-size three-dimensional water window x-ray microscope that combines wide-field transmission x-ray microscopy with tomographic reconstruction techniques, and observed bio-medical samples to evaluate its applicability to life science research fields. It consists of a condenser and an objective grazing incidence Wolter type I mirror, an electron-impact type oxygen Kα x-ray source, and a back-illuminated CCD for x-ray imaging. A spatial resolution limit of around 1.0 line pairs per micrometer was obtained for two-dimensional transmission images, and 1-μm scale three-dimensional fine structures were resolved.

Automatic classification of retinal three-dimensional optical coherence tomography images using principal component analysis network with composite kernels

NASA Astrophysics Data System (ADS)

Fang, Leyuan; Wang, Chong; Li, Shutao; Yan, Jun; Chen, Xiangdong; Rabbani, Hossein

2017-11-01

We present an automatic method, termed as the principal component analysis network with composite kernel (PCANet-CK), for the classification of three-dimensional (3-D) retinal optical coherence tomography (OCT) images. Specifically, the proposed PCANet-CK method first utilizes the PCANet to automatically learn features from each B-scan of the 3-D retinal OCT images. Then, multiple kernels are separately applied to a set of very important features of the B-scans and these kernels are fused together, which can jointly exploit the correlations among features of the 3-D OCT images. Finally, the fused (composite) kernel is incorporated into an extreme learning machine for the OCT image classification. We tested our proposed algorithm on two real 3-D spectral domain OCT (SD-OCT) datasets (of normal subjects and subjects with the macular edema and age-related macular degeneration), which demonstrated its effectiveness.

Virtual finger boosts three-dimensional imaging and microsurgery as well as terabyte volume image visualization and analysis.

PubMed

Peng, Hanchuan; Tang, Jianyong; Xiao, Hang; Bria, Alessandro; Zhou, Jianlong; Butler, Victoria; Zhou, Zhi; Gonzalez-Bellido, Paloma T; Oh, Seung W; Chen, Jichao; Mitra, Ananya; Tsien, Richard W; Zeng, Hongkui; Ascoli, Giorgio A; Iannello, Giulio; Hawrylycz, Michael; Myers, Eugene; Long, Fuhui

2014-07-11

Three-dimensional (3D) bioimaging, visualization and data analysis are in strong need of powerful 3D exploration techniques. We develop virtual finger (VF) to generate 3D curves, points and regions-of-interest in the 3D space of a volumetric image with a single finger operation, such as a computer mouse stroke, or click or zoom from the 2D-projection plane of an image as visualized with a computer. VF provides efficient methods for acquisition, visualization and analysis of 3D images for roundworm, fruitfly, dragonfly, mouse, rat and human. Specifically, VF enables instant 3D optical zoom-in imaging, 3D free-form optical microsurgery, and 3D visualization and annotation of terabytes of whole-brain image volumes. VF also leads to orders of magnitude better efficiency of automated 3D reconstruction of neurons and similar biostructures over our previous systems. We use VF to generate from images of 1,107 Drosophila GAL4 lines a projectome of a Drosophila brain.

Theoretical analysis for the optical deformation of emulsion droplets.

PubMed

Tapp, David; Taylor, Jonathan M; Lubansky, Alex S; Bain, Colin D; Chakrabarti, Buddhapriya

2014-02-24

We propose a theoretical framework to predict the three-dimensional shapes of optically deformed micron-sized emulsion droplets with ultra-low interfacial tension. The resulting shape and size of the droplet arises out of a balance between the interfacial tension and optical forces. Using an approximation of the laser field as a Gaussian beam, working within the Rayleigh-Gans regime and assuming isotropic surface energy at the oil-water interface, we numerically solve the resulting shape equations to elucidate the three-dimensional droplet geometry. We obtain a plethora of shapes as a function of the number of optical tweezers, their laser powers and positions, surface tension, initial droplet size and geometry. Experimentally, two-dimensional droplet silhouettes have been imaged from above, but their full side-on view has not been observed and reported for current optical configurations. This experimental limitation points to ambiguity in differentiating between droplets having the same two-dimensional projection but with disparate three-dimensional shapes. Our model elucidates and quantifies this difference for the first time. We also provide a dimensionless number that indicates the shape transformation (ellipsoidal to dumbbell) at a value ≈ 1.0, obtained by balancing interfacial tension and laser forces, substantiated using a data collapse.

Theoretical Analysis for the Optical Shaping of Emulsion Droplets

NASA Astrophysics Data System (ADS)

Tapp, David; Taylor, Jonathan; Lubanksy, Alex; Bain, Colin; Chakrabarti, Buddhapriya

2014-03-01

Motivated by recent experimental observations, I discuss a theoretical framework to predict the three-dimensional shapes of optically deformed micron-sized emulsion droplets with ultra-low interfacial tension. The resulting shape and size of the droplet arises out of a balance between the interfacial tension and optical forces. Using an approximation of the laser field as a Gaussian beam, working within the Rayleigh-Gans regime and beyond, and assuming isotropic surface energy at the oil-water interface, the resulting shape equations are numerically solved to elucidate the three-dimensional droplet geometry. A plethora of shapes as a function of the number of optical tweezers, their laser powers and positions, surface tension, initial droplet size and geometry are obtained. Experimentally, two-dimensional emulsion droplet silhouettes have been imaged from above, but their full side-on view has not been observed and reported for current optical configurations. This experimental limitation points to ambiguity in differentiating between droplets having the same two-dimensional projection but with disparate three-dimensional shapes. The model I present elucidates and quantifies this difference for the first time. Supported by funding from EPSRC via grant EP/I013377/1.

Nanometric holograms based on a topological insulator material

PubMed Central

Yue, Zengji; Xue, Gaolei; Liu, Juan; Wang, Yongtian; Gu, Min

2017-01-01

Holography has extremely extensive applications in conventional optical instruments spanning optical microscopy and imaging, three-dimensional displays and metrology. To integrate holography with modern low-dimensional electronic devices, holograms need to be thinned to a nanometric scale. However, to keep a pronounced phase shift modulation, the thickness of holograms has been generally limited to the optical wavelength scale, which hinders their integration with ultrathin electronic devices. Here, we break this limit and achieve 60 nm holograms using a topological insulator material. We discover that nanometric topological insulator thin films act as an intrinsic optical resonant cavity due to the unequal refractive indices in their metallic surfaces and bulk. The resonant cavity leads to enhancement of phase shifts and thus the holographic imaging. Our work paves a way towards integrating holography with flat electronic devices for optical imaging, data storage and information security. PMID:28516906

Advances in three-dimensional integral imaging: sensing, display, and applications [Invited].

PubMed

Xiao, Xiao; Javidi, Bahram; Martinez-Corral, Manuel; Stern, Adrian

2013-02-01

Three-dimensional (3D) sensing and imaging technologies have been extensively researched for many applications in the fields of entertainment, medicine, robotics, manufacturing, industrial inspection, security, surveillance, and defense due to their diverse and significant benefits. Integral imaging is a passive multiperspective imaging technique, which records multiple two-dimensional images of a scene from different perspectives. Unlike holography, it can capture a scene such as outdoor events with incoherent or ambient light. Integral imaging can display a true 3D color image with full parallax and continuous viewing angles by incoherent light; thus it does not suffer from speckle degradation. Because of its unique properties, integral imaging has been revived over the past decade or so as a promising approach for massive 3D commercialization. A series of key articles on this topic have appeared in the OSA journals, including Applied Optics. Thus, it is fitting that this Commemorative Review presents an overview of literature on physical principles and applications of integral imaging. Several data capture configurations, reconstruction, and display methods are overviewed. In addition, applications including 3D underwater imaging, 3D imaging in photon-starved environments, 3D tracking of occluded objects, 3D optical microscopy, and 3D polarimetric imaging are reviewed.

Four-dimensional Microscope-Integrated Optical Coherence Tomography to Visualize Suture Depth in Strabismus Surgery.

PubMed

Pasricha, Neel D; Bhullar, Paramjit K; Shieh, Christine; Carrasco-Zevallos, Oscar M; Keller, Brenton; Izatt, Joseph A; Toth, Cynthia A; Freedman, Sharon F; Kuo, Anthony N

2017-02-14

The authors report the use of swept-source microscope-integrated optical coherence tomography (SS-MIOCT), capable of live four-dimensional (three-dimensional across time) intraoperative imaging, to directly visualize suture depth during lateral rectus resection. Key surgical steps visualized in this report included needle depth during partial and full-thickness muscle passes along with scleral passes. [J Pediatr Ophthalmol Strabismus. 2017;54:e1-e5.]. Copyright 2017, SLACK Incorporated.

3-D endoscopic imaging using plenoptic camera.

PubMed

Le, Hanh N D; Decker, Ryan; Opferman, Justin; Kim, Peter; Krieger, Axel; Kang, Jin U

2016-06-01

Three-dimensional endoscopic imaging using plenoptic technique combined with F-matching algorithm has been pursued in this study. A custom relay optics was designed to integrate a commercial surgical straight endoscope with a plenoptic camera.

Dual-dimensional microscopy: real-time in vivo three-dimensional observation method using high-resolution light-field microscopy and light-field display.

PubMed

Kim, Jonghyun; Moon, Seokil; Jeong, Youngmo; Jang, Changwon; Kim, Youngmin; Lee, Byoungho

2018-06-01

Here, we present dual-dimensional microscopy that captures both two-dimensional (2-D) and light-field images of an in-vivo sample simultaneously, synthesizes an upsampled light-field image in real time, and visualizes it with a computational light-field display system in real time. Compared with conventional light-field microscopy, the additional 2-D image greatly enhances the lateral resolution at the native object plane up to the diffraction limit and compensates for the image degradation at the native object plane. The whole process from capturing to displaying is done in real time with the parallel computation algorithm, which enables the observation of the sample's three-dimensional (3-D) movement and direct interaction with the in-vivo sample. We demonstrate a real-time 3-D interactive experiment with Caenorhabditis elegans. (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

Remote assessment of diabetic foot ulcers using a novel wound imaging system.

PubMed

Bowling, Frank L; King, Laurie; Paterson, James A; Hu, Jingyi; Lipsky, Benjamin A; Matthews, David R; Boulton, Andrew J M

2011-01-01

Telemedicine allows experts to assess patients in remote locations, enabling quality convenient, cost-effective care. To help assess foot wounds remotely, we investigated the reliability of a novel optical imaging system employing a three-dimensional camera and disposable optical marker. We first examined inter- and intraoperator measurement variability (correlation coefficient) of five clinicians examining three different wounds. Then, to assess of the system's ability to identify key clinically relevant features, we had two clinicians evaluate 20 different wounds at two centers, recording observations on a standardized form. Three other clinicians recorded their observations using only the corresponding three-dimensional images. Using the in-person assessment as the criterion standard, we assessed concordance of the remote with in-person assessments. Measurement variation of area was 3.3% for intraoperator and 11.9% for interoperator; difference in clinician opinion about wound boundary location was significant. Overall agreement for remote vs. in-person assessments was good, but was lowest on the subjective clinical assessments, e.g., value of debridement to improve healing. Limitations of imaging included inability to show certain characteristics, e.g., moistness or exudation. Clinicians gave positive feedback on visual fidelity. This pilot study showed that a clinician viewing only the three-dimensional images could accurately measure and assess a diabetic foot wound remotely. © 2010 by the Wound Healing Society.

Three-dimensional refractive index and fluorescence tomography using structured illumination (Conference Presentation)

NASA Astrophysics Data System (ADS)

Park, GwangSik; Shin, SeungWoo; Kim, Kyoohyun; Park, YongKeun

2017-02-01

Optical diffraction tomography (ODT) has been an emerging optical technique for label-free imaging of three-dimensional (3-D) refractive index (RI) distribution of biological samples. ODT employs interferometric microscopy for measuring multiple holograms of samples with various incident angles, from which the Fourier diffraction theorem reconstructs the 3-D RI distribution of samples from retrieved complex optical fields. Since the RI value is linearly proportional to the protein concentration of biological samples where the proportional coefficient is called as refractive index increment (RII), reconstructed 3-D RI tomograms provide precise structural and biochemical information of individual biological samples. Because most proteins have similar RII value, however, ODT has limited molecular specificity, especially for imaging eukaryotic cells having various types of proteins and subcellular organelles. Here, we present an ODT system combined with structured illumination microscopy which can measure the 3-D RI distribution of biological samples as well as 3-D super-resolution fluorescent images in the same optical setup. A digital micromirror device (DMD) controls the incident angle of the illumination beam for tomogram reconstruction, and the same DMD modulates the structured illumination pattern of the excitation beam for super-resolution fluorescent imaging. We first validate the proposed method for simultaneous optical diffraction tomographic imaging and super-resolution fluorescent imaging of fluorescent beads. The proposed method is also exploited for various biological samples.

Multiple interpretations of a pair of images of a surface

NASA Astrophysics Data System (ADS)

Longuet-Higgins, H. C.

1988-07-01

It is known that, if two optical images of a visually textured surface, projected from finitely separated viewpoints, allow more than one three-dimensional interpretation, then the surface must be part of a quadric passing through the two viewpoints. It is here shown that this quadric is either a plane or a ruled surface of a type first considered by Maybank (1985) in a study of ambiguous optic flow fields. In the latter case, three is the maximum number of distinct interpretations that the two images can sustain.

Real-time three-dimensional optical coherence tomography image-guided core-needle biopsy system.

PubMed

Kuo, Wei-Cheng; Kim, Jongsik; Shemonski, Nathan D; Chaney, Eric J; Spillman, Darold R; Boppart, Stephen A

2012-06-01

Advances in optical imaging modalities, such as optical coherence tomography (OCT), enable us to observe tissue microstructure at high resolution and in real time. Currently, core-needle biopsies are guided by external imaging modalities such as ultrasound imaging and x-ray computed tomography (CT) for breast and lung masses, respectively. These image-guided procedures are frequently limited by spatial resolution when using ultrasound imaging, or by temporal resolution (rapid real-time feedback capabilities) when using x-ray CT. One feasible approach is to perform OCT within small gauge needles to optically image tissue microstructure. However, to date, no system or core-needle device has been developed that incorporates both three-dimensional OCT imaging and tissue biopsy within the same needle for true OCT-guided core-needle biopsy. We have developed and demonstrate an integrated core-needle biopsy system that utilizes catheter-based 3-D OCT for real-time image-guidance for target tissue localization, imaging of tissue immediately prior to physical biopsy, and subsequent OCT imaging of the biopsied specimen for immediate assessment at the point-of-care. OCT images of biopsied ex vivo tumor specimens acquired during core-needle placement are correlated with corresponding histology, and computational visualization of arbitrary planes within the 3-D OCT volumes enables feedback on specimen tissue type and biopsy quality. These results demonstrate the potential for using real-time 3-D OCT for needle biopsy guidance by imaging within the needle and tissue during biopsy procedures.

Three-dimensional fuse deposition modeling of tissue-simulating phantom for biomedical optical imaging

NASA Astrophysics Data System (ADS)

Dong, Erbao; Zhao, Zuhua; Wang, Minjie; Xie, Yanjun; Li, Shidi; Shao, Pengfei; Cheng, Liuquan; Xu, Ronald X.

2015-12-01

Biomedical optical devices are widely used for clinical detection of various tissue anomalies. However, optical measurements have limited accuracy and traceability, partially owing to the lack of effective calibration methods that simulate the actual tissue conditions. To facilitate standardized calibration and performance evaluation of medical optical devices, we develop a three-dimensional fuse deposition modeling (FDM) technique for freeform fabrication of tissue-simulating phantoms. The FDM system uses transparent gel wax as the base material, titanium dioxide (TiO2) powder as the scattering ingredient, and graphite powder as the absorption ingredient. The ingredients are preheated, mixed, and deposited at the designated ratios layer-by-layer to simulate tissue structural and optical heterogeneities. By printing the sections of human brain model based on magnetic resonance images, we demonstrate the capability for simulating tissue structural heterogeneities. By measuring optical properties of multilayered phantoms and comparing with numerical simulation, we demonstrate the feasibility for simulating tissue optical properties. By creating a rat head phantom with embedded vasculature, we demonstrate the potential for mimicking physiologic processes of a living system.

SERODS optical data storage with parallel signal transfer

DOEpatents

Vo-Dinh, Tuan

2003-09-02

Surface-enhanced Raman optical data storage (SERODS) systems having increased reading and writing speeds, that is, increased data transfer rates, are disclosed. In the various SERODS read and write systems, the surface-enhanced Raman scattering (SERS) data is written and read using a two-dimensional process called parallel signal transfer (PST). The various embodiments utilize laser light beam excitation of the SERODS medium, optical filtering, beam imaging, and two-dimensional light detection. Two- and three-dimensional SERODS media are utilized. The SERODS write systems employ either a different laser or a different level of laser power.

SERODS optical data storage with parallel signal transfer

DOEpatents

Vo-Dinh, Tuan

2003-06-24

Surface-enhanced Raman optical data storage (SERODS) systems having increased reading and writing speeds, that is, increased data transfer rates, are disclosed. In the various SERODS read and write systems, the surface-enhanced Raman scattering (SERS) data is written and read using a two-dimensional process called parallel signal transfer (PST). The various embodiments utilize laser light beam excitation of the SERODS medium, optical filtering, beam imaging, and two-dimensional light detection. Two- and three-dimensional SERODS media are utilized. The SERODS write systems employ either a different laser or a different level of laser power.

Wavefront measurement using computational adaptive optics.

PubMed

South, Fredrick A; Liu, Yuan-Zhi; Bower, Andrew J; Xu, Yang; Carney, P Scott; Boppart, Stephen A

2018-03-01

In many optical imaging applications, it is necessary to correct for aberrations to obtain high quality images. Optical coherence tomography (OCT) provides access to the amplitude and phase of the backscattered optical field for three-dimensional (3D) imaging samples. Computational adaptive optics (CAO) modifies the phase of the OCT data in the spatial frequency domain to correct optical aberrations without using a deformable mirror, as is commonly done in hardware-based adaptive optics (AO). This provides improvement of image quality throughout the 3D volume, enabling imaging across greater depth ranges and in highly aberrated samples. However, the CAO aberration correction has a complicated relation to the imaging pupil and is not a direct measurement of the pupil aberrations. Here we present new methods for recovering the wavefront aberrations directly from the OCT data without the use of hardware adaptive optics. This enables both computational measurement and correction of optical aberrations.

Three-dimensional imaging and photostimulation by remote-focusing and holographic light patterning

PubMed Central

Anselmi, Francesca; Ventalon, Cathie; Bègue, Aurélien; Ogden, David; Emiliani, Valentina

2011-01-01

Access to three-dimensional structures in the brain is fundamental to probe signal processing at multiple levels, from integration of synaptic inputs to network activity mapping. Here, we present an optical method for independent three-dimensional photoactivation and imaging by combination of digital holography with remote-focusing. We experimentally demonstrate compensation of spherical aberration for out-of-focus imaging in a range of at least 300 μm, as well as scanless imaging along oblique planes. We apply this method to perform functional imaging along tilted dendrites of hippocampal pyramidal neurons in brain slices, after photostimulation by multiple spots glutamate uncaging. By bringing extended portions of tilted dendrites simultaneously in-focus, we monitor the spatial extent of dendritic calcium signals, showing a shift from a widespread to a spatially confined response upon blockage of voltage-gated Na+ channels. PMID:22074779

3-D endoscopic imaging using plenoptic camera

PubMed Central

Le, Hanh N. D.; Decker, Ryan; Opferman, Justin; Kim, Peter; Krieger, Axel

2017-01-01

Three-dimensional endoscopic imaging using plenoptic technique combined with F-matching algorithm has been pursued in this study. A custom relay optics was designed to integrate a commercial surgical straight endoscope with a plenoptic camera. PMID:29276806

A highly accurate symmetric optical flow based high-dimensional nonlinear spatial normalization of brain images.

PubMed

Wen, Ying; Hou, Lili; He, Lianghua; Peterson, Bradley S; Xu, Dongrong

2015-05-01

Spatial normalization plays a key role in voxel-based analyses of brain images. We propose a highly accurate algorithm for high-dimensional spatial normalization of brain images based on the technique of symmetric optical flow. We first construct a three dimension optical model with the consistency assumption of intensity and consistency of the gradient of intensity under a constraint of discontinuity-preserving spatio-temporal smoothness. Then, an efficient inverse consistency optical flow is proposed with aims of higher registration accuracy, where the flow is naturally symmetric. By employing a hierarchical strategy ranging from coarse to fine scales of resolution and a method of Euler-Lagrange numerical analysis, our algorithm is capable of registering brain images data. Experiments using both simulated and real datasets demonstrated that the accuracy of our algorithm is not only better than that of those traditional optical flow algorithms, but also comparable to other registration methods used extensively in the medical imaging community. Moreover, our registration algorithm is fully automated, requiring a very limited number of parameters and no manual intervention. Copyright © 2015 Elsevier Inc. All rights reserved.

Large-angle illumination STEM: Toward three-dimensional atom-by-atom imaging

DOE PAGES

Ishikawa, Ryo; Lupini, Andrew R.; Hinuma, Yoyo; ...

2014-11-26

To completely understand and control materials and their properties, it is of critical importance to determine their atomic structures in all three dimensions. Recent revolutionary advances in electron optics – the inventions of geometric and chromatic aberration correctors as well as electron source monochromators – have provided fertile ground for performing optical depth sectioning at atomic-scale dimensions. In this study we theoretically demonstrate the imaging of top/sub-surface atomic structures and identify the depth of single dopants, single vacancies and the other point defects within materials by large-angle illumination scanning transmission electron microscopy (LAI-STEM). The proposed method also allows us tomore » measure specimen properties such as thickness or three-dimensional surface morphology using observations from a single crystallographic orientation.« less

Dimensional metrology of lab-on-a-chip internal structures: a comparison of optical coherence tomography with confocal fluorescence microscopy.

PubMed

Reyes, D R; Halter, M; Hwang, J

2015-07-01

The characterization of internal structures in a polymeric microfluidic device, especially of a final product, will require a different set of optical metrology tools than those traditionally used for microelectronic devices. We demonstrate that optical coherence tomography (OCT) imaging is a promising technique to characterize the internal structures of poly(methyl methacrylate) devices where the subsurface structures often cannot be imaged by conventional wide field optical microscopy. The structural details of channels in the devices were imaged with OCT and analyzed with an in-house written ImageJ macro in an effort to identify the structural details of the channel. The dimensional values obtained with OCT were compared with laser-scanning confocal microscopy images of channels filled with a fluorophore solution. Attempts were also made using confocal reflectance and interferometry microscopy to measure the channel dimensions, but artefacts present in the images precluded quantitative analysis. OCT provided the most accurate estimates for the channel height based on an analysis of optical micrographs obtained after destructively slicing the channel with a microtome. OCT may be a promising technique for the future of three-dimensional metrology of critical internal structures in lab-on-a-chip devices because scans can be performed rapidly and noninvasively prior to their use. © 2015 The Authors Journal of Microscopy © 2015 Royal Microscopical Society.

Parity-Time Symmetric Nonlocal Metasurfaces: All-Angle Negative Refraction and Volumetric Imaging

NASA Astrophysics Data System (ADS)

Monticone, Francesco; Valagiannopoulos, Constantinos A.; Alù, Andrea

2016-10-01

Lens design for focusing and imaging has been optimized through centuries of developments; however, conventional lenses, even in their most ideal realizations, still suffer from fundamental limitations, such as limits in resolution and the presence of optical aberrations, which are inherent to the laws of refraction. In addition, volume-to-volume imaging of three-dimensional regions of space is not possible with systems based on conventional refractive optics, which are inherently limited to plane-to-plane imaging. Although some of these limitations have been at least theoretically relaxed with the advent of metamaterials, several challenges still stand in the way of ideal imaging of three-dimensional regions of space. Here, we show that the concept of parity-time symmetry, combined with tailored nonlocal responses, enables overcoming some of these challenges, and we propose the design of a loss-immune, linear, transversely invariant, planarized metamaterial lens, with reduced aberrations and the potential to realize volume-to-volume imaging.

Image Size Scalable Full-parallax Coloured Three-dimensional Video by Electronic Holography

NASA Astrophysics Data System (ADS)

Sasaki, Hisayuki; Yamamoto, Kenji; Ichihashi, Yasuyuki; Senoh, Takanori

2014-02-01

In electronic holography, various methods have been considered for using multiple spatial light modulators (SLM) to increase the image size. In a previous work, we used a monochrome light source for a method that located an optical system containing lens arrays and other components in front of multiple SLMs. This paper proposes a colourization technique for that system based on time division multiplexing using laser light sources of three colours (red, green, and blue). The experimental device we constructed was able to perform video playback (20 fps) in colour of full parallax holographic three-dimensional (3D) images with an image size of 63 mm and a viewing-zone angle of 5.6 degrees without losing any part of the 3D image.

Plenoptic Imaging of a Three Dimensional Cold Atom Cloud

NASA Astrophysics Data System (ADS)

Lott, Gordon

2017-04-01

A plenoptic imaging system is capable of sampling the rays of light in a volume, both spatially and angularly, providing information about the three dimensional (3D) volume being imaged. The extraction of the 3D structure of a cold atom cloud is demonstrated, using a single plenoptic camera and a single image. The reconstruction is tested against a reference image and the results discussed along with the capabilities and limitations of the imaging system. This capability is useful when the 3D distribution of the atoms is desired, such as determining the shape of an atom trap, particularly when there is limited optical access. Gratefully acknowledge support from AFRL.

Three-dimensional ophthalmic optical coherence tomography with a refraction correction algorithm

NASA Astrophysics Data System (ADS)

Zawadzki, Robert J.; Leisser, Christoph; Leitgeb, Rainer; Pircher, Michael; Fercher, Adolf F.

2003-10-01

We built an optical coherence tomography (OCT) system with a rapid scanning optical delay (RSOD) line, which allows probing full axial eye length. The system produces Three-dimensional (3D) data sets that are used to generate 3D tomograms of the model eye. The raw tomographic data were processed by an algorithm, which is based on Snell"s law to correct the interface positions. The Zernike polynomials representation of the interfaces allows quantitative wave aberration measurements. 3D images of our results are presented to illustrate the capabilities of the system and the algorithm performance. The system allows us to measure intra-ocular distances.

Recent advances in Optical Computed Tomography (OCT) imaging system for three dimensional (3D) radiotherapy dosimetry

NASA Astrophysics Data System (ADS)

Rahman, Ahmad Taufek Abdul; Farah Rosli, Nurul; Zain, Shafirah Mohd; Zin, Hafiz M.

2018-01-01

Radiotherapy delivery techniques for cancer treatment are becoming more complex and highly focused, to enable accurate radiation dose delivery to the cancerous tissue and minimum dose to the healthy tissue adjacent to tumour. Instrument to verify the complex dose delivery in radiotherapy such as optical computed tomography (OCT) measures the dose from a three-dimensional (3D) radiochromic dosimeter to ensure the accuracy of the radiotherapy beam delivery to the patient. OCT measures the optical density in radiochromic material that changes predictably upon exposure to radiotherapy beams. OCT systems have been developed using a photodiode and charged coupled device (CCD) as the detector. The existing OCT imaging systems have limitation in terms of the accuracy and the speed of the measurement. Advances in on-pixel intelligence CMOS image sensor (CIS) will be exploited in this work to replace current detector in OCT imaging systems. CIS is capable of on-pixel signal processing at a very fast imaging speed (over several hundred images per second) that will allow improvement in the 3D measurement of the optical density. The paper will review 3D radiochromic dosimeters and OCT systems developed and discuss how CMOS based OCT imaging will provide accurate and fast optical density measurements in 3D. The paper will also discuss the configuration of the CMOS based OCT developed in this work and how it may improve the existing OCT system.

Hand-held optical imager (Gen-2): improved instrumentation and target detectability

PubMed Central

Gonzalez, Jean; DeCerce, Joseph; Erickson, Sarah J.; Martinez, Sergio L.; Nunez, Annie; Roman, Manuela; Traub, Barbara; Flores, Cecilia A.; Roberts, Seigbeh M.; Hernandez, Estrella; Aguirre, Wenceslao; Kiszonas, Richard

2012-01-01

Abstract. Hand-held optical imagers are developed by various researchers towards reflectance-based spectroscopic imaging of breast cancer. Recently, a Gen-1 handheld optical imager was developed with capabilities to perform two-dimensional (2-D) spectroscopic as well as three-dimensional (3-D) tomographic imaging studies. However, the imager was bulky with poor surface contact (∼30%) along curved tissues, and limited sensitivity to detect targets consistently. Herein, a Gen-2 hand-held optical imager that overcame the above limitations of the Gen-1 imager has been developed and the instrumentation described. The Gen-2 hand-held imager is less bulky, portable, and has improved surface contact (∼86%) on curved tissues. Additionally, the forked probe head design is capable of simultaneous bilateral reflectance imaging of both breast tissues, and also transillumination imaging of a single breast tissue. Experimental studies were performed on tissue phantoms to demonstrate the improved sensitivity in detecting targets using the Gen-2 imager. The improved instrumentation of the Gen-2 imager allowed detection of targets independent of their location with respect to the illumination points, unlike in Gen-1 imager. The developed imager has potential for future clinical breast imaging with enhanced sensitivity, via both reflectance and transillumination imaging. PMID:23224163

Three-dimensional surface profile intensity correction for spatially modulated imaging

NASA Astrophysics Data System (ADS)

Gioux, Sylvain; Mazhar, Amaan; Cuccia, David J.; Durkin, Anthony J.; Tromberg, Bruce J.; Frangioni, John V.

2009-05-01

We describe a noncontact profile correction technique for quantitative, wide-field optical measurement of tissue absorption (μa) and reduced scattering (μs') coefficients, based on geometric correction of the sample's Lambertian (diffuse) reflectance intensity. Because the projection of structured light onto an object is the basis for both phase-shifting profilometry and modulated imaging, we were able to develop a single instrument capable of performing both techniques. In so doing, the surface of the three-dimensional object could be acquired and used to extract the object's optical properties. The optical properties of flat polydimethylsiloxane (silicone) phantoms with homogenous tissue-like optical properties were extracted, with and without profilometry correction, after vertical translation and tilting of the phantoms at various angles. Objects having a complex shape, including a hemispheric silicone phantom and human fingers, were acquired and similarly processed, with vascular constriction of a finger being readily detectable through changes in its optical properties. Using profilometry correction, the accuracy of extracted absorption and reduced scattering coefficients improved from two- to ten-fold for surfaces having height variations as much as 3 cm and tilt angles as high as 40 deg. These data lay the foundation for employing structured light for quantitative imaging during surgery.

Three-dimensional microscopic tomographic imagings of the cataract in a human lens in vivo

NASA Astrophysics Data System (ADS)

Masters, Barry R.

1998-10-01

The problem of three-dimensional visualization of a human lens in vivo has been solved by a technique of volume rendering a transformed series of 60 rotated Scheimpflug (a dual slit reflected light microscope) digital images. The data set was obtained by rotating the Scheimpflug camera about the optic axis of the lens in 3 degree increments. The transformed set of optical sections were first aligned to correct for small eye movements, and then rendered into a volume reconstruction with volume rendering computer graphics techniques. To help visualize the distribution of lens opacities (cataracts) in the living, human lens the intensity of light scattering was pseudocolor coded and the cataract opacities were displayed as a movie.

Dynamics of optically levitated microparticles in vacuum placed in 2D and 3D optical potentials possessing orbital angular momentum

NASA Astrophysics Data System (ADS)

Arita, Yoshihiko; Mazilu, Michael; Chen, Mingzhou; Vettenburg, Tom; Auñón, Juan M.; Wright, Ewan M.; Dholakia, Kishan

2017-04-01

We demonstrate the transfer of orbital angular momentum to optically levitated microparticles in vacuum [1]. We prepare two-dimensional and three-dimensional optical potentials. In the former case the microparticle is placed within a Laguerre-Gaussian beam and orbits the annular beam profile with increasing angular velocity as the air drag coefficient is reduced. We explore the particle dynamics as a function of the topological charge of the levitating beam. Our results reveal that there is a fundamental limit to the orbital angular momentum that may be transferred to a trapped particle, dependent upon the beam parameters and inertial forces present. This effect was predicted theoretically [2] and can be understood considering the underlying dynamics arising from the link between the magnitude of the azimuthal index and the beam radius [3]. Whilst a Laguerre-Gaussian beam scales in size with azimuthal index `, recently we have created a "perfect" vortex beam whose radial intensity profile and radius are both independent of topological charge [4, 5]. As the Fourier transform of a perfect vortex yields a Bessel beam. Imaging a perfect vortex, with its subsequent propagation thus realises a complex three dimensional optical field. In this scenario we load individual silica microparticles into this field and observe their trajectories. The optical gradient and scattering forces interplay with the inertial and gravitational forces acting on the trapped particle, including the rotational degrees of freedom. As a result the trapped microparticle exhibits a complex three dimensional motion that includes a periodic orbital motion between the Bessel and the perfect vortex beam. We are able to determine the three dimensional optical potential in situ by tracking the particle. This first demonstration of trapping microparticles within a complex three dimensional optical potential in vacuum opens up new possibilities for fundamental studies of many-body dynamics, mesoscopic entanglement [6, 7], and optical binding [8, 9].

Large size three-dimensional video by electronic holography using multiple spatial light modulators

PubMed Central

Sasaki, Hisayuki; Yamamoto, Kenji; Wakunami, Koki; Ichihashi, Yasuyuki; Oi, Ryutaro; Senoh, Takanori

2014-01-01

In this paper, we propose a new method of using multiple spatial light modulators (SLMs) to increase the size of three-dimensional (3D) images that are displayed using electronic holography. The scalability of images produced by the previous method had an upper limit that was derived from the path length of the image-readout part. We were able to produce larger colour electronic holographic images with a newly devised space-saving image-readout optical system for multiple reflection-type SLMs. This optical system is designed so that the path length of the image-readout part is half that of the previous method. It consists of polarization beam splitters (PBSs), half-wave plates (HWPs), and polarizers. We used 16 (4 × 4) 4K×2K-pixel SLMs for displaying holograms. The experimental device we constructed was able to perform 20 fps video reproduction in colour of full-parallax holographic 3D images with a diagonal image size of 85 mm and a horizontal viewing-zone angle of 5.6 degrees. PMID:25146685

Large size three-dimensional video by electronic holography using multiple spatial light modulators.

PubMed

Sasaki, Hisayuki; Yamamoto, Kenji; Wakunami, Koki; Ichihashi, Yasuyuki; Oi, Ryutaro; Senoh, Takanori

2014-08-22

In this paper, we propose a new method of using multiple spatial light modulators (SLMs) to increase the size of three-dimensional (3D) images that are displayed using electronic holography. The scalability of images produced by the previous method had an upper limit that was derived from the path length of the image-readout part. We were able to produce larger colour electronic holographic images with a newly devised space-saving image-readout optical system for multiple reflection-type SLMs. This optical system is designed so that the path length of the image-readout part is half that of the previous method. It consists of polarization beam splitters (PBSs), half-wave plates (HWPs), and polarizers. We used 16 (4 × 4) 4K×2K-pixel SLMs for displaying holograms. The experimental device we constructed was able to perform 20 fps video reproduction in colour of full-parallax holographic 3D images with a diagonal image size of 85 mm and a horizontal viewing-zone angle of 5.6 degrees.

Noncontact three-dimensional quantitative profiling of fast aspheric lenses by optical coherence tomography

NASA Astrophysics Data System (ADS)

Goud, Bujagouni Karthik; Udupa, Dinesh Venkatesh; Prathap, Chilakala; Shinde, Deepak Dilip; Rao, Kompalli Divakar; Sahoo, Naba Kishore

2016-12-01

The use of optical coherence tomography (OCT) for noncontact three-dimensional aspheric lens profiling and retrieval of aspheric surface parameters is demonstrated. Two commercially available aspheric lenses with different focal length-to-diameter ratio have been imaged using OCT, and the measured optical path length distribution has been least square fitted with the aspheric lens surface retrieving the radius of curvature, aspheric constant, and conic constants. The refractive index of these lenses has also been measured referencing with a standard Zerodur glass flat. The fitted aspheric surface coefficients of the lenses are in close agreement with the manufacturer's values, thus, envisaging the potential of OCT in rapid screening, testing of aspheric lenses, and other micro-optical components such as those used in illumination optics.

THREE-DIMENSIONAL RANDOM ACCESS MULTIPHOTON MICROSCOPY FOR FAST FUNCTIONAL IMAGING OF NEURONAL ACTIVITY

PubMed Central

Reddy, Gaddum Duemani; Kelleher, Keith; Fink, Rudy; Saggau, Peter

2009-01-01

The dynamic ability of neuronal dendrites to shape and integrate synaptic responses is the hallmark of information processing in the brain. Effectively studying this phenomenon requires concurrent measurements at multiple sites on live neurons. Significant progress has been made by optical imaging systems which combine confocal and multiphoton microscopy with inertia-free laser scanning. However, all systems developed to date restrict fast imaging to two dimensions. This severely limits the extent to which neurons can be studied, since they represent complex three-dimensional (3D) structures. Here we present a novel imaging system that utilizes a unique arrangement of acousto-optic deflectors to steer a focused ultra-fast laser beam to arbitrary locations in 3D space without moving the objective lens. As we demonstrate, this highly versatile random-access multiphoton microscope supports functional imaging of complex 3D cellular structures such as neuronal dendrites or neural populations at acquisition rates on the order of tens of kilohertz. PMID:18432198

Geometrical Reasoning in Wave Situations: The Case of Light Diffraction and Coherent Illumination Optical Imaging

ERIC Educational Resources Information Center

Maurines, Laurence

2010-01-01

This particular study is part of a research programme on the difficulties encountered by students when learning about wave phenomena in a three-dimensional medium in the absence or presence of obstacles. It focuses on how students reason in situations in which wave optics need to be used: diffraction of light by an aperture, imaging in the…

A fish on the hunt, observed neuron by neuron

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

2010-01-01

This three-dimensional microscopy image reveals an output neuron of the optic tectum lighting up in response to visual information from the retina. The scientists used this state-of-the-art imaging technology to learn how neurons in the optic tectum take visual information and convert it into an output that drives action. More information: http://newscenter.lbl.gov/feature-stories/2010/10/29/zebrafish-vision/

Three-Dimensional Root Phenotyping with a Novel Imaging and Software Platform1[C][W][OA

PubMed Central

Clark, Randy T.; MacCurdy, Robert B.; Jung, Janelle K.; Shaff, Jon E.; McCouch, Susan R.; Aneshansley, Daniel J.; Kochian, Leon V.

2011-01-01

A novel imaging and software platform was developed for the high-throughput phenotyping of three-dimensional root traits during seedling development. To demonstrate the platform’s capacity, plants of two rice (Oryza sativa) genotypes, Azucena and IR64, were grown in a transparent gellan gum system and imaged daily for 10 d. Rotational image sequences consisting of 40 two-dimensional images were captured using an optically corrected digital imaging system. Three-dimensional root reconstructions were generated and analyzed using a custom-designed software, RootReader3D. Using the automated and interactive capabilities of RootReader3D, five rice root types were classified and 27 phenotypic root traits were measured to characterize these two genotypes. Where possible, measurements from the three-dimensional platform were validated and were highly correlated with conventional two-dimensional measurements. When comparing gellan gum-grown plants with those grown under hydroponic and sand culture, significant differences were detected in morphological root traits (P < 0.05). This highly flexible platform provides the capacity to measure root traits with a high degree of spatial and temporal resolution and will facilitate novel investigations into the development of entire root systems or selected components of root systems. In combination with the extensive genetic resources that are now available, this platform will be a powerful resource to further explore the molecular and genetic determinants of root system architecture. PMID:21454799

Images of Axial Objects

ERIC Educational Resources Information Center

Rabal, Hector; Cap, Nelly; Trivi, Marcelo

2011-01-01

Imaging of three-dimensional objects by lenses and mirrors is sometimes poorly indicated in textbooks and can be incorrectly drawn. We stress a need to clarify the concept of longitudinal magnification, with simulated images illustrating distortions introduced along the optical axis. We consider all possible positions of the object for both a…

Three-Dimensional Cataract Crystalline Lens Imaging With Swept-Source Optical Coherence Tomography.

PubMed

de Castro, Alberto; Benito, Antonio; Manzanera, Silvestre; Mompeán, Juan; Cañizares, Belén; Martínez, David; Marín, Jose María; Grulkowski, Ireneusz; Artal, Pablo

2018-02-01

To image, describe, and characterize different features visible in the crystalline lens of older adults with and without cataract when imaged three-dimensionally with a swept-source optical coherence tomography (SS-OCT) system. We used a new SS-OCT laboratory prototype designed to enhance the visualization of the crystalline lens and imaged the entire anterior segment of both eyes in two groups of participants: patients scheduled to undergo cataract surgery, n = 17, age range 36 to 91 years old, and volunteers without visual complains, n = 14, age range 20 to 81 years old. Pre-cataract surgery patients were also clinically graded according to the Lens Opacification Classification System III. The three-dimensional location and shape of the visible opacities were compared with the clinical grading. Hypo- and hyperreflective features were visible in the lens of all pre-cataract surgery patients and in some of the older adults in the volunteer group. When the clinical examination revealed cortical or subcapsular cataracts, hyperreflective features were visible either in the cortex parallel to the surfaces of the lens or in the posterior pole. Other type of opacities that appeared as hyporeflective localized features were identified in the cortex of the lens. The OCT signal in the nucleus of the crystalline lens correlated with the nuclear cataract clinical grade. A dedicated OCT is a useful tool to study in vivo the subtle opacities in the cataractous crystalline lens, revealing its position and size three-dimensionally. The use of these images allows obtaining more detailed information on the age-related changes leading to cataract.

A stereo remote sensing feature selection method based on artificial bee colony algorithm

NASA Astrophysics Data System (ADS)

Yan, Yiming; Liu, Pigang; Zhang, Ye; Su, Nan; Tian, Shu; Gao, Fengjiao; Shen, Yi

2014-05-01

To improve the efficiency of stereo information for remote sensing classification, a stereo remote sensing feature selection method is proposed in this paper presents, which is based on artificial bee colony algorithm. Remote sensing stereo information could be described by digital surface model (DSM) and optical image, which contain information of the three-dimensional structure and optical characteristics, respectively. Firstly, three-dimensional structure characteristic could be analyzed by 3D-Zernike descriptors (3DZD). However, different parameters of 3DZD could descript different complexity of three-dimensional structure, and it needs to be better optimized selected for various objects on the ground. Secondly, features for representing optical characteristic also need to be optimized. If not properly handled, when a stereo feature vector composed of 3DZD and image features, that would be a lot of redundant information, and the redundant information may not improve the classification accuracy, even cause adverse effects. To reduce information redundancy while maintaining or improving the classification accuracy, an optimized frame for this stereo feature selection problem is created, and artificial bee colony algorithm is introduced for solving this optimization problem. Experimental results show that the proposed method can effectively improve the computational efficiency, improve the classification accuracy.

Vectorial point spread function and optical transfer function in oblique plane imaging.

PubMed

Kim, Jeongmin; Li, Tongcang; Wang, Yuan; Zhang, Xiang

2014-05-05

Oblique plane imaging, using remote focusing with a tilted mirror, enables direct two-dimensional (2D) imaging of any inclined plane of interest in three-dimensional (3D) specimens. It can image real-time dynamics of a living sample that changes rapidly or evolves its structure along arbitrary orientations. It also allows direct observations of any tilted target plane in an object of which orientational information is inaccessible during sample preparation. In this work, we study the optical resolution of this innovative wide-field imaging method. Using the vectorial diffraction theory, we formulate the vectorial point spread function (PSF) of direct oblique plane imaging. The anisotropic lateral resolving power caused by light clipping from the tilted mirror is theoretically analyzed for all oblique angles. We show that the 2D PSF in oblique plane imaging is conceptually different from the inclined 2D slice of the 3D PSF in conventional lateral imaging. Vectorial optical transfer function (OTF) of oblique plane imaging is also calculated by the fast Fourier transform (FFT) method to study effects of oblique angles on frequency responses.

Three-dimensional in vitro cancer spheroid models for Photodynamic Therapy: Strengths and Opportunities

NASA Astrophysics Data System (ADS)

Evans, Conor

2015-03-01

Three dimensional, in vitro spheroid cultures offer considerable utility for the development and testing of anticancer photodynamic therapy regimens. More complex than monolayer cultures, three-dimensional spheroid systems replicate many of the important cell-cell and cell-matrix interactions that modulate treatment response in vivo. Simple enough to be grown by the thousands and small enough to be optically interrogated, spheroid cultures lend themselves to high-content and high-throughput imaging approaches. These advantages have enabled studies investigating photosensitizer uptake, spatiotemporal patterns of therapeutic response, alterations in oxygen diffusion and consumption during therapy, and the exploration of mechanisms that underlie therapeutic synergy. The use of quantitative imaging methods, in particular, has accelerated the pace of three-dimensional in vitro photodynamic therapy studies, enabling the rapid compilation of multiple treatment response parameters in a single experiment. Improvements in model cultures, the creation of new molecular probes of cell state and function, and innovations in imaging toolkits will be important for the advancement of spheroid culture systems for future photodynamic therapy studies.

Three-dimensional deformation of orthodontic brackets

PubMed Central

Melenka, Garrett W; Nobes, David S; Major, Paul W

2013-01-01

Braces are used by orthodontists to correct the misalignment of teeth in the mouth. Archwire rotation is a particular procedure used to correct tooth inclination. Wire rotation can result in deformation to the orthodontic brackets, and an orthodontic torque simulator has been designed to examine this wire–bracket interaction. An optical technique has been employed to measure the deformation due to size and geometric constraints of the orthodontic brackets. Images of orthodontic brackets are collected using a stereo microscope and two charge-coupled device cameras, and deformation of orthodontic brackets is measured using a three-dimensional digital image correlation technique. The three-dimensional deformation of orthodontic brackets will be evaluated. The repeatability of the three-dimensional digital image correlation measurement method was evaluated by performing 30 archwire rotation tests using the same bracket and archwire. Finally, five Damon 3MX and five In-Ovation R self-ligating brackets will be compared using this technique to demonstrate the effect of archwire rotation on bracket design. PMID:23762201

Three-dimensional deformation of orthodontic brackets.

PubMed

Melenka, Garrett W; Nobes, David S; Major, Paul W; Carey, Jason P

2013-01-01

Braces are used by orthodontists to correct the misalignment of teeth in the mouth. Archwire rotation is a particular procedure used to correct tooth inclination. Wire rotation can result in deformation to the orthodontic brackets, and an orthodontic torque simulator has been designed to examine this wire-bracket interaction. An optical technique has been employed to measure the deformation due to size and geometric constraints of the orthodontic brackets. Images of orthodontic brackets are collected using a stereo microscope and two charge-coupled device cameras, and deformation of orthodontic brackets is measured using a three-dimensional digital image correlation technique. The three-dimensional deformation of orthodontic brackets will be evaluated. The repeatability of the three-dimensional digital image correlation measurement method was evaluated by performing 30 archwire rotation tests using the same bracket and archwire. Finally, five Damon 3MX and five In-Ovation R self-ligating brackets will be compared using this technique to demonstrate the effect of archwire rotation on bracket design.

Comparing three-dimensional serial optical coherence tomography histology to MRI imaging in the entire mouse brain

NASA Astrophysics Data System (ADS)

Castonguay, Alexandre; Lefebvre, Joël; Pouliot, Philippe; Lesage, Frédéric

2018-01-01

An automated serial histology setup combining optical coherence tomography (OCT) imaging with vibratome sectioning was used to image eight wild type mouse brains. The datasets resulted in thousands of volumetric tiles resolved at a voxel size of (4.9×4.9×6.5) μm3 stitched back together to give a three-dimensional map of the brain from which a template OCT brain was obtained. To assess deformation caused by tissue sectioning, reconstruction algorithms, and fixation, OCT datasets were compared to both in vivo and ex vivo magnetic resonance imaging (MRI) imaging. The OCT brain template yielded a highly detailed map of the brain structure, with a high contrast in white matter fiber bundles and was highly resemblant to the in vivo MRI template. Brain labeling using the Allen brain framework showed little variation in regional brain volume among imaging modalities with no statistical differences. The high correspondence between the OCT template brain and its in vivo counterpart demonstrates the potential of whole brain histology to validate in vivo imaging.

Measurement of spatial refractive index distributions of fusion spliced optical fibers by digital holographic microtomography

NASA Astrophysics Data System (ADS)

Pan, Feng; Deng, Yating; Ma, Xichao; Xiao, Wen

2017-11-01

Digital holographic microtomography is improved and applied to the measurements of three-dimensional refractive index distributions of fusion spliced optical fibers. Tomographic images are reconstructed from full-angle phase projection images obtained with a setup-rotation approach, in which the laser source, the optical system and the image sensor are arranged on an optical breadboard and synchronously rotated around the fixed object. For retrieving high-quality tomographic images, a numerical method is proposed to compensate the unwanted movements of the object in the lateral, axial and vertical directions during rotation. The compensation is implemented on the two-dimensional phase images instead of the sinogram. The experimental results exhibit distinctly the internal structures of fusion splices between a single-mode fiber and other fibers, including a multi-mode fiber, a panda polarization maintaining fiber, a bow-tie polarization maintaining fiber and a photonic crystal fiber. In particular, the internal structure distortion in the fusion areas can be intuitively observed, such as the expansion of the stress zones of polarization maintaining fibers, the collapse of the air holes of photonic crystal fibers, etc.

Three-dimensional Optical Coherence Tomography for Optical Biopsy of Lymph Nodes and Assessment of Metastatic Disease

PubMed Central

John, Renu; Adie, Steven G.; Chaney, Eric J.; Marjanovic, Marina; Tangella, Krishnarao V.; Boppart, Stephen A.

2013-01-01

Background Numerous techniques have been developed for localizing lymph nodes before surgical resection and for their histological assessment. Nondestructive high-resolution transcapsule optical imaging of lymph nodes offers the potential for in situ assessment of metastatic involvement, potentially during surgical procedures. Methods Three-dimensional optical coherence tomography (3-D OCT) was used for imaging and assessing resected popliteal lymph nodes from a preclinical rat metastatic tumor model over a 9-day time-course study after tumor induction. The spectral-domain OCT system utilized a center wavelength of 800 nm, provided axial and transverse resolutions of 3 and 12 µm, respectively, and performed imaging at 10,000 axial scans per second. Results OCT is capable of providing high-resolution labelfree images of intact lymph node microstructure based on intrinsic optical scattering properties with penetration depths of ~1–2 mm. The results demonstrate that OCT is capable of differentiating normal, reactive, and metastatic lymph nodes based on microstructural changes. The optical scattering and structural changes revealed by OCT from day 3 to day 9 after the injection of tumor cells into the lymphatic system correlate with inflammatory and immunological changes observed in the capsule, precortical regions, follicles, and germination centers found during histopathology. Conclusions We report for the first time a longitudinal study of 3-D transcapsule OCT imaging of intact lymph nodes demonstrating microstructural changes during metastatic infiltration. These results demonstrate the potential of OCT as a technique for intraoperative, real-time in situ 3-D optical biopsy of lymph nodes for the intraoperative staging of cancer. PMID:22688663

Three-dimensional optical reconstruction of vocal fold kinematics using high-speed video with a laser projection system

PubMed Central

Luegmair, Georg; Mehta, Daryush D.; Kobler, James B.; Döllinger, Michael

2015-01-01

Vocal fold kinematics and its interaction with aerodynamic characteristics play a primary role in acoustic sound production of the human voice. Investigating the temporal details of these kinematics using high-speed videoendoscopic imaging techniques has proven challenging in part due to the limitations of quantifying complex vocal fold vibratory behavior using only two spatial dimensions. Thus, we propose an optical method of reconstructing the superior vocal fold surface in three spatial dimensions using a high-speed video camera and laser projection system. Using stereo-triangulation principles, we extend the camera-laser projector method and present an efficient image processing workflow to generate the three-dimensional vocal fold surfaces during phonation captured at 4000 frames per second. Initial results are provided for airflow-driven vibration of an ex vivo vocal fold model in which at least 75% of visible laser points contributed to the reconstructed surface. The method captures the vertical motion of the vocal folds at a high accuracy to allow for the computation of three-dimensional mucosal wave features such as vibratory amplitude, velocity, and asymmetry. PMID:26087485

Analysis of eletrectrohydrodynamic jetting using multifunctional and three-dimensional tomography

NASA Astrophysics Data System (ADS)

Ko, Han Seo; Nguyen, Xuan Hung; Lee, Soo-Hong; Kim, Young Hyun

2013-11-01

Three-dimensional optical tomography technique was developed to reconstruct three-dimensional flow fields using a set of two-dimensional shadowgraphic images and normal gray images. From three high speed cameras, which were positioned at an offset angle of 45° relative to one another, number, size and location of electrohydrodynamic jets with respect to the nozzle position were analyzed using shadowgraphic tomography employing a multiplicative algebraic reconstruction technique (MART). Additionally, a flow field inside cone-shaped liquid (Taylor cone) which was induced under electric field was also observed using a simultaneous multiplicative algebraic reconstruction technique (SMART) for reconstructing intensities of particle light and combining with a three-dimensional cross correlation. Various velocity fields of a circulating flow inside the cone-shaped liquid due to different physico-chemical properties of liquid and applied voltages were also investigated. This work supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Korean government (MEST) (No. S-2011-0023457).

Three-dimensional ghost imaging lidar via sparsity constraint

NASA Astrophysics Data System (ADS)

Gong, Wenlin; Zhao, Chengqiang; Yu, Hong; Chen, Mingliang; Xu, Wendong; Han, Shensheng

2016-05-01

Three-dimensional (3D) remote imaging attracts increasing attentions in capturing a target’s characteristics. Although great progress for 3D remote imaging has been made with methods such as scanning imaging lidar and pulsed floodlight-illumination imaging lidar, either the detection range or application mode are limited by present methods. Ghost imaging via sparsity constraint (GISC), enables the reconstruction of a two-dimensional N-pixel image from much fewer than N measurements. By GISC technique and the depth information of targets captured with time-resolved measurements, we report a 3D GISC lidar system and experimentally show that a 3D scene at about 1.0 km range can be stably reconstructed with global measurements even below the Nyquist limit. Compared with existing 3D optical imaging methods, 3D GISC has the capability of both high efficiency in information extraction and high sensitivity in detection. This approach can be generalized in nonvisible wavebands and applied to other 3D imaging areas.

Depth measurements through controlled aberrations of projected patterns.

PubMed

Birch, Gabriel C; Tyo, J Scott; Schwiegerling, Jim

2012-03-12

Three-dimensional displays have become increasingly present in consumer markets. However, the ability to capture three-dimensional images in space confined environments and without major modifications to current cameras is uncommon. Our goal is to create a simple modification to a conventional camera that allows for three dimensional reconstruction. We require such an imaging system have imaging and illumination paths coincident. Furthermore, we require that any three-dimensional modification to a camera also permits full resolution 2D image capture.Here we present a method of extracting depth information with a single camera and aberrated projected pattern. A commercial digital camera is used in conjunction with a projector system with astigmatic focus to capture images of a scene. By using an astigmatic projected pattern we can create two different focus depths for horizontal and vertical features of a projected pattern, thereby encoding depth. By designing an aberrated projected pattern, we are able to exploit this differential focus in post-processing designed to exploit the projected pattern and optical system. We are able to correlate the distance of an object at a particular transverse position from the camera to ratios of particular wavelet coefficients.We present our information regarding construction, calibration, and images produced by this system. The nature of linking a projected pattern design and image processing algorithms will be discussed.

High-speed imaging with endoscopic optical coherence tomography using bending vibration of optical fiber

NASA Astrophysics Data System (ADS)

Isago, Ryoichi; Nakamura, Kentaro

2009-10-01

In this report, we propose an endoscopic scanner head for optical coherence tomography (OCT) using bending vibration of an optical fiber. The optical fiber is attached to the center of a cylindrical piezoelectric actuator with four outer electrodes, and the voltages with the phase shift of π/2 are applied to the electrodes to excite a circular vibration of the fiber end. The output light from the fiber end is collimated by a lens, and deflected by 90 degrees using a cone mirror. The collimated light is scanned along the circumference of the endoscope due to the vibration of the optical fiber end. We made a prototype scanner head of 7.0 mm in outer diameter, and demonstrated tomographic imaging of tubular objects. The circumferential scan is carried out at 1 kHz which is the frequency of the fiber vibration, while the radial (depth) scan is performed at 20 kHz by the wavelength sweep of the light source. Two-dimensional OCT images were obtained in a short measuring time of 5 ms (flame rate of 200 fps), and three-dimensional dynamic imaging were demonstrated.

Optical computed tomography for spatially isotropic four-dimensional imaging of live single cells

PubMed Central

Kelbauskas, Laimonas; Shetty, Rishabh; Cao, Bin; Wang, Kuo-Chen; Smith, Dean; Wang, Hong; Chao, Shi-Hui; Gangaraju, Sandhya; Ashcroft, Brian; Kritzer, Margaret; Glenn, Honor; Johnson, Roger H.; Meldrum, Deirdre R.

2017-01-01

Quantitative three-dimensional (3D) computed tomography (CT) imaging of living single cells enables orientation-independent morphometric analysis of the intricacies of cellular physiology. Since its invention, x-ray CT has become indispensable in the clinic for diagnostic and prognostic purposes due to its quantitative absorption-based imaging in true 3D that allows objects of interest to be viewed and measured from any orientation. However, x-ray CT has not been useful at the level of single cells because there is insufficient contrast to form an image. Recently, optical CT has been developed successfully for fixed cells, but this technology called Cell-CT is incompatible with live-cell imaging due to the use of stains, such as hematoxylin, that are not compatible with cell viability. We present a novel development of optical CT for quantitative, multispectral functional 4D (three spatial + one spectral dimension) imaging of living single cells. The method applied to immune system cells offers truly isotropic 3D spatial resolution and enables time-resolved imaging studies of cells suspended in aqueous medium. Using live-cell optical CT, we found a heterogeneous response to mitochondrial fission inhibition in mouse macrophages and differential basal remodeling of small (0.1 to 1 fl) and large (1 to 20 fl) nuclear and mitochondrial structures on a 20- to 30-s time scale in human myelogenous leukemia cells. Because of its robust 3D measurement capabilities, live-cell optical CT represents a powerful new tool in the biomedical research field. PMID:29226240

Three-dimensional displacement measurement of image point by point-diffraction interferometry

NASA Astrophysics Data System (ADS)

He, Xiao; Chen, Lingfeng; Meng, Xiaojie; Yu, Lei

2018-01-01

This paper presents a method for measuring the three-dimensional (3-D) displacement of an image point based on point-diffraction interferometry. An object Point-light-source (PLS) interferes with a fixed PLS and its interferograms are captured by an exit pupil. When the image point of the object PLS is slightly shifted to a new position, the wavefront of the image PLS changes. And its interferograms also change. Processing these figures (captured before and after the movement), the wavefront difference of the image PLS can be obtained and it contains the information of three-dimensional (3-D) displacement of the image PLS. However, the information of its three-dimensional (3-D) displacement cannot be calculated until the distance between the image PLS and the exit pupil is calibrated. Therefore, we use a plane-parallel-plate with a known refractive index and thickness to determine this distance, which is based on the Snell's law for small angle of incidence. Thus, since the distance between the exit pupil and the image PLS is a known quantity, the 3-D displacement of the image PLS can be simultaneously calculated through two interference measurements. Preliminary experimental results indicate that its relative error is below 0.3%. With the ability to accurately locate an image point (whatever it is real or virtual), a fiber point-light-source can act as the reticle by itself in optical measurement.

Techniques of noninvasive optical tomographic imaging

NASA Astrophysics Data System (ADS)

Rosen, Joseph; Abookasis, David; Gokhler, Mark

2006-01-01

Recently invented methods of optical tomographic imaging through scattering and absorbing media are presented. In one method, the three-dimensional structure of an object hidden between two biological tissues is recovered from many noisy speckle pictures obtained on the output of a multi-channeled optical imaging system. Objects are recovered from many speckled images observed by a digital camera through two stereoscopic microlens arrays. Each microlens in each array generates a speckle image of the object buried between the layers. In the computer each image is Fourier transformed jointly with an image of the speckled point-like source captured under the same conditions. A set of the squared magnitudes of the Fourier-transformed pictures is accumulated to form a single average picture. This final picture is again Fourier transformed, resulting in the three-dimensional reconstruction of the hidden object. In the other method, the effect of spatial longitudinal coherence is used for imaging through an absorbing layer with different thickness, or different index of refraction, along the layer. The technique is based on synthesis of multiple peak spatial degree of coherence. This degree of coherence enables us to scan simultaneously different sample points on different altitudes, and thus decreases the acquisition time. The same multi peak degree of coherence is also used for imaging through the absorbing layer. Our entire experiments are performed with a quasi-monochromatic light source. Therefore problems of dispersion and inhomogeneous absorption are avoided.

Investigations on the change of texture of plant cells due to preservative treatments by digital holographic microscopy

NASA Astrophysics Data System (ADS)

Vora, Priyanka; Anand, Arun

2014-10-01

Texture change is observed in preserved fruits and vegetables. Responsible factors for texture change during preservative treatments are cell morphology, cell wall structure, cell turger, water content and some biochemical components, and also the environmental conditions. Digital Holographic microscopy (DHM) is a quantitative phase contrast imaging technique, which provides three dimensional optical thickness profiles of transparent specimen. Using DHM the morphology of plant cells preserved by refrigeration or stored in vinegar or in sodium chloride can be obtained. This information about the spatio-temporal evolution of optical volume and thickness can be an important tool in area of food processing. Also from the three dimensional images, the texture of the cell can be retrieved and can be investigated under varying conditions.

Three dimensional imaging detector employing wavelength-shifting optical fibers

DOEpatents

Worstell, William A.

1997-01-01

A novel detector element structure and method for its use is provided. In a preferred embodiment, one or more inorganic scintillating crystals are coupled through wavelength shifting optical fibers (WLSFs) to position sensitive photomultipliers (PS-PMTs). The superior detector configuration in accordance with this invention is designed for an array of applications in high spatial resolution gamma ray sensing with particular application to SPECT, PET and PVI imaging systems. The design provides better position resolution than prior art devices at a lower total cost. By employing wavelength shifting fibers (WLSFs), the sensor configuration of this invention can operate with a significant reduction in the number of photomultipliers and electronics channels, while potentially improving the resolution of the system by allowing three dimensional reconstruction of energy deposition positions.

Three dimensional imaging detector employing wavelength-shifting optical fibers

DOEpatents

Worstell, W.A.

1997-02-04

A novel detector element structure and method for its use is provided. In a preferred embodiment, one or more inorganic scintillating crystals are coupled through wavelength shifting optical fibers (WLSFs) to position sensitive photomultipliers (PS-PMTs). The superior detector configuration in accordance with this invention is designed for an array of applications in high spatial resolution gamma ray sensing with particular application to SPECT, PET and PVI imaging systems. The design provides better position resolution than prior art devices at a lower total cost. By employing wavelength shifting fibers (WLSFs), the sensor configuration of this invention can operate with a significant reduction in the number of photomultipliers and electronics channels, while potentially improving the resolution of the system by allowing three dimensional reconstruction of energy deposition positions. 11 figs.

Three-dimensional DNA image cytometry by optical projection tomographic microscopy for early cancer diagnosis.

PubMed

Agarwal, Nitin; Biancardi, Alberto M; Patten, Florence W; Reeves, Anthony P; Seibel, Eric J

2014-04-01

Aneuploidy is typically assessed by flow cytometry (FCM) and image cytometry (ICM). We used optical projection tomographic microscopy (OPTM) for assessing cellular DNA content using absorption and fluorescence stains. OPTM combines some of the attributes of both FCM and ICM and generates isometric high-resolution three-dimensional (3-D) images of single cells. Although the depth of field of the microscope objective was in the submicron range, it was extended by scanning the objective's focal plane. The extended depth of field image is similar to a projection in a conventional x-ray computed tomography. These projections were later reconstructed using computed tomography methods to form a 3-D image. We also present an automated method for 3-D nuclear segmentation. Nuclei of chicken, trout, and triploid trout erythrocyte were used to calibrate OPTM. Ratios of integrated optical densities extracted from 50 images of each standard were compared to ratios of DNA indices from FCM. A comparison of mean square errors with thionin, hematoxylin, Feulgen, and SYTOX green was done. Feulgen technique was preferred as it showed highest stoichiometry, least variance, and preserved nuclear morphology in 3-D. The addition of this quantitative biomarker could further strengthen existing classifiers and improve early diagnosis of cancer using 3-D microscopy.

Three-dimensional imaging of artificial fingerprint by optical coherence tomography

NASA Astrophysics Data System (ADS)

Larin, Kirill V.; Cheng, Yezeng

2008-03-01

Fingerprint recognition is one of the popular used methods of biometrics. However, due to the surface topography limitation, fingerprint recognition scanners are easily been spoofed, e.g. using artificial fingerprint dummies. Thus, biometric fingerprint identification devices need to be more accurate and secure to deal with different fraudulent methods including dummy fingerprints. Previously, we demonstrated that Optical Coherence Tomography (OCT) images revealed the presence of the artificial fingerprints (made from different household materials, such as cement and liquid silicone rubber) at all times, while the artificial fingerprints easily spoofed the commercial fingerprint reader. Also we demonstrated that an analysis of the autocorrelation of the OCT images could be used in automatic recognition systems. Here, we exploited the three-dimensional (3D) imaging of the artificial fingerprint by OCT to generate vivid 3D image for both the artificial fingerprint layer and the real fingerprint layer beneath. With the reconstructed 3D image, it could not only point out whether there exists an artificial material, which is intended to spoof the scanner, above the real finger, but also could provide the hacker's fingerprint. The results of these studies suggested that Optical Coherence Tomography could be a powerful real-time noninvasive method for accurate identification of artificial fingerprints real fingerprints as well.

Image improvement and three-dimensional reconstruction using holographic image processing

NASA Technical Reports Server (NTRS)

Stroke, G. W.; Halioua, M.; Thon, F.; Willasch, D. H.

1977-01-01

Holographic computing principles make possible image improvement and synthesis in many cases of current scientific and engineering interest. Examples are given for the improvement of resolution in electron microscopy and 3-D reconstruction in electron microscopy and X-ray crystallography, following an analysis of optical versus digital computing in such applications.

Effect of Nanohole Spacing on the Self-Imaging Phenomenon Created by the Three-Dimensional Propagation of Light through Periodic Nanohole Arrays

PubMed Central

Chowdhury, Mustafa H.; Lindquist, Nathan C.; Lesuffleur, Antoine; Oh, Sang-Hyun; Lakowicz, Joseph R.; Ray, Krishanu

2013-01-01

We present a detailed study of the inter-nanohole distance that governs the self-imaging phenomenon created by the three-dimensional propagation of light through periodic nanohole arrays on plasmonic substrates. We used scanning near-field optical microscopy (SNOM) to map the light intensity distributions at various heights above 10×10 nanohole arrays of varying pitch sizes on silver films. Our results suggest the inter-hole spacing has to be greater than the wavelength of the incident light to create the self-imaging phenomenon. We also present Finite-Difference Time-Domain (FDTD) calculations which show qualitative corroboration of our experimental results. Both our experimental and FDTD results show that the self-imaging phenomenon is more pronounced for structures with larger pitch sizes. We believe this self-imaging phenomenon is related to the Talbot imaging effect that has also been modified by a plasmonic component and can potentially be used to provide the basis for a new class of optical microscopes. PMID:24416456

Combined optical coherence tomography and hyper-spectral imaging using a double clad fiber coupler

NASA Astrophysics Data System (ADS)

Guay-Lord, Robin; Lurie, Kristen L.; Attendu, Xavier; Mageau, Lucas; Godbout, Nicolas; Ellerbee Bowden, Audrey K.; Strupler, Mathias; Boudoux, Caroline

2016-03-01

This proceedings shows the combination of Optical Coherence Tomography (OCT) and Hyper-Spectral Imaging (HSI) using a double-clad optical fiber. The single mode core of the fiber is used to transmit OCT signals, while the cladding, with its large collection area, provides an efficient way to capture the reflectance spectrum of the sample. The combination of both methods enables three-dimensional acquisition of sample morphology with OCT, enhanced by the molecular information contained in its hyper-spectral image. We believe that the combination of these techniques could result in endoscopes with enhanced tissue identification capability.

Three-dimensional non-destructive optical evaluation of laser-processing performance using optical coherence tomography.

PubMed

Kim, Youngseop; Choi, Eun Seo; Kwak, Wooseop; Shin, Yongjin; Jung, Woonggyu; Ahn, Yeh-Chan; Chen, Zhongping

2008-06-01

We demonstrate the use of optical coherence tomography (OCT) as a non-destructive diagnostic tool for evaluating laser-processing performance by imaging the features of a pit and a rim. A pit formed on a material at different laser-processing conditions is imaged using both a conventional scanning electron microscope (SEM) and OCT. Then using corresponding images, the geometrical characteristics of the pit are analyzed and compared. From the results, we could verify the feasibility and the potential of the application of OCT to the monitoring of the laser-processing performance.

All sky imaging Fabry-Perot spectrometer for optical investigation of the upper atmosphere

NASA Astrophysics Data System (ADS)

Sekar, R.; Gurubaran, S.; Sridharan, R.

1993-06-01

A simple optical design, keeping in view of the available components, has been worked out to develop the 'all sky imaging Fabry-Perot spectrometer' to study the spatial structures in thermospheric winds and temperature. This system comprises three subsystems, namely, (1) field widening front-end optics, (2) high resolution Fabry-Perot spectrometer and (3) a two-dimensional detector. The design details of the above imaging spectrometer that has been commissioned for routine observations from Mt. Abu along with the first results on OI 6300 A airglow emission are presented and discussed.

Three-dimensional non-destructive optical evaluation of laser-processing performance using optical coherence tomography

PubMed Central

Kim, Youngseop; Choi, Eun Seo; Kwak, Wooseop; Shin, Yongjin; Jung, Woonggyu; Ahn, Yeh-Chan; Chen, Zhongping

2014-01-01

We demonstrate the use of optical coherence tomography (OCT) as a non-destructive diagnostic tool for evaluating laser-processing performance by imaging the features of a pit and a rim. A pit formed on a material at different laser-processing conditions is imaged using both a conventional scanning electron microscope (SEM) and OCT. Then using corresponding images, the geometrical characteristics of the pit are analyzed and compared. From the results, we could verify the feasibility and the potential of the application of OCT to the monitoring of the laser-processing performance. PMID:24932051

Three-dimensional imaging of biological cells with picosecond ultrasonics

NASA Astrophysics Data System (ADS)

Danworaphong, Sorasak; Tomoda, Motonobu; Matsumoto, Yuki; Matsuda, Osamu; Ohashi, Toshiro; Watanabe, Hiromu; Nagayama, Masafumi; Gohara, Kazutoshi; Otsuka, Paul H.; Wright, Oliver B.

2015-04-01

We use picosecond ultrasonics to image animal cells in vitro—a bovine aortic endothelial cell and a mouse adipose cell—fixed to Ti-coated sapphire. Tightly focused ultrashort laser pulses generate and detect GHz acoustic pulses, allowing three-dimensional imaging (x, y, and t) of the ultrasonic propagation in the cells with ˜1 μm lateral and ˜150 nm depth resolutions. Time-frequency representations of the continuous-wavelet-transform amplitude of the optical reflectivity variations inside and outside the cells show GHz Brillouin oscillations, allowing the average sound velocities of the cells and their ultrasonic attenuation to be obtained as well as the average bulk moduli.

A head-mounted compressive three-dimensional display system with polarization-dependent focus switching

NASA Astrophysics Data System (ADS)

Lee, Chang-Kun; Moon, Seokil; Lee, Byounghyo; Jeong, Youngmo; Lee, Byoungho

2016-10-01

A head-mounted compressive three-dimensional (3D) display system is proposed by combining polarization beam splitter (PBS), fast switching polarization rotator and micro display with high pixel density. According to the polarization state of the image controlled by polarization rotator, optical path of image in the PBS can be divided into transmitted and reflected components. Since optical paths of each image are spatially separated, it is possible to independently focus both images at different depth positions. Transmitted p-polarized and reflected s-polarized images can be focused by convex lens and mirror, respectively. When the focal lengths of the convex lens and mirror are properly determined, two image planes can be located in intended positions. The geometrical relationship is easily modulated by replacement of the components. The fast switching of polarization realizes the real-time operation of multi-focal image planes with a single display panel. Since it is possible to conserve the device characteristic of single panel, the high image quality, reliability and uniformity can be retained. For generating 3D images, layer images for compressive light field display between two image planes are calculated. Since the display panel with high pixel density is adopted, high quality 3D images are reconstructed. In addition, image degradation by diffraction between physically stacked display panels can be mitigated. Simple optical configuration of the proposed system is implemented and the feasibility of the proposed method is verified through experiments.

Super resolution imaging of HER2 gene amplification

NASA Astrophysics Data System (ADS)

Okada, Masaya; Kubo, Takuya; Masumoto, Kanako; Iwanaga, Shigeki

2016-02-01

HER2 positive breast cancer is currently examined by counting HER2 genes using fluorescence in situ hybridization (FISH)-stained breast carcinoma samples. In this research, two-dimensional super resolution fluorescence microscopy based on stochastic optical reconstruction microscopy (STORM), with a spatial resolution of approximately 20 nm in the lateral direction, was used to more precisely distinguish and count HER2 genes in a FISH-stained tissue section. Furthermore, by introducing double-helix point spread function (DH-PSF), an optical phase modulation technique, to super resolution microscopy, three-dimensional images were obtained of HER2 in a breast carcinoma sample approximately 4 μm thick.

Doppler imaging using spectrally-encoded endoscopy

PubMed Central

Yelin, Dvir; Bouma, B. E.; Rosowsky, J. J.; Tearney, G. J.

2009-01-01

The capability to image tissue motion such as blood flow through an endoscope could have many applications in medicine. Spectrally encoded endoscopy (SEE) is a recently introduced technique that utilizes a single optical fiber and miniature diffractive optics to obtain endoscopic images through small diameter probes. Using spectral-domain interferometry, SEE is furthermore capable of three-dimensional volume imaging at video rates. Here we show that by measuring relative spectral phases, this technology can additionally measure Doppler shifts. Doppler SEE is demonstrated in flowing Intralipid phantoms and vibrating middle ear ossicles. PMID:18795020

Three-dimensional Hessian matrix-based quantitative vascular imaging of rat iris with optical-resolution photoacoustic microscopy in vivo

NASA Astrophysics Data System (ADS)

Zhao, Huangxuan; Wang, Guangsong; Lin, Riqiang; Gong, Xiaojing; Song, Liang; Li, Tan; Wang, Wenjia; Zhang, Kunya; Qian, Xiuqing; Zhang, Haixia; Li, Lin; Liu, Zhicheng; Liu, Chengbo

2018-04-01

For the diagnosis and evaluation of ophthalmic diseases, imaging and quantitative characterization of vasculature in the iris are very important. The recently developed photoacoustic imaging, which is ultrasensitive in imaging endogenous hemoglobin molecules, provides a highly efficient label-free method for imaging blood vasculature in the iris. However, the development of advanced vascular quantification algorithms is still needed to enable accurate characterization of the underlying vasculature. We have developed a vascular information quantification algorithm by adopting a three-dimensional (3-D) Hessian matrix and applied for processing iris vasculature images obtained with a custom-built optical-resolution photoacoustic imaging system (OR-PAM). For the first time, we demonstrate in vivo 3-D vascular structures of a rat iris with a the label-free imaging method and also accurately extract quantitative vascular information, such as vessel diameter, vascular density, and vascular tortuosity. Our results indicate that the developed algorithm is capable of quantifying the vasculature in the 3-D photoacoustic images of the iris in-vivo, thus enhancing the diagnostic capability of the OR-PAM system for vascular-related ophthalmic diseases in vivo.

Data processing from lobster eye type optics

NASA Astrophysics Data System (ADS)

Nentvich, Ondrej; Stehlikova, Veronika; Urban, Martin; Hudec, Rene; Sieger, Ladislav

2017-05-01

Wolter I optics are commonly used for imaging in X-Ray spectrum. This system uses two reflections, and at higher energies, this system is not so much efficient but has a very good optical resolution. Here is another type of optics Lobster Eye, which is using also two reflections for focusing rays in Schmidt's or Angel's arrangement. Here is also possible to use Lobster eye optics as two one dimensional independent optics. This paper describes advantages of one dimensional and two dimensional Lobster Eye optics in Schmidt's arrangement and its data processing - find out a number of sources in wide field of view. Two dimensional (2D) optics are suitable to detect the number of point X-ray sources and their magnitude, but it is necessary to expose for a long time because a 2D system has much lower transitivity, due to double reflection, compared to one dimensional (1D) optics. Not only for this reason, two 1D optics are better to use for lower magnitudes of sources. In this case, additional image processing is necessary to achieve a 2D image. This article describes of approach an image reconstruction and advantages of two 1D optics without significant losses of transitivity.

Optical coherence tomography imaging for evaluating the photo biomodulation effects on tissue regeneration in the oral cavity

NASA Astrophysics Data System (ADS)

Gimbel, Craig B.

2008-03-01

Optical Coherence Tomography (OCT) is a noninvasive method for imaging dental microstructure which has the potential of evaluating the health of periodontal tissue. OCT provides an "optical biopsy" of tissue 2-3 mm in depth. Optical biopsy is a measurement of the localized optical properties based on tissue type and pathology. This sixth modality of imaging was pioneered at Lawrence Livermore National Laboratory. OCT is based on the optical scattering signatures within tissue structure. With the use of a broad spectrum bandwidth light source, high resolution images, 10 times the resolution of radiographs, can detect important tissue interfaces within the periodontal sulcus and its' relationship to the attachment apparatus of the tooth. Multiple cross-sectional tomograms can be stacked to create two and three dimensional images providing information as to health of periodontal tissue important to both the clinician and researcher.

A new three-dimensional nonscanning laser imaging system based on the illumination pattern of a point-light-source array

NASA Astrophysics Data System (ADS)

Xia, Wenze; Ma, Yayun; Han, Shaokun; Wang, Yulin; Liu, Fei; Zhai, Yu

2018-06-01

One of the most important goals of research on three-dimensional nonscanning laser imaging systems is the improvement of the illumination system. In this paper, a new three-dimensional nonscanning laser imaging system based on the illumination pattern of a point-light-source array is proposed. This array is obtained using a fiber array connected to a laser array with each unit laser having independent control circuits. This system uses a point-to-point imaging process, which is realized using the exact corresponding optical relationship between the point-light-source array and a linear-mode avalanche photodiode array detector. The complete working process of this system is explained in detail, and the mathematical model of this system containing four equations is established. A simulated contrast experiment and two real contrast experiments which use the simplified setup without a laser array are performed. The final results demonstrate that unlike a conventional three-dimensional nonscanning laser imaging system, the proposed system meets all the requirements of an eligible illumination system. Finally, the imaging performance of this system is analyzed under defocusing situations, and analytical results show that the system has good defocusing robustness and can be easily adjusted in real applications.

OPTICAL PROCESSING OF INFORMATION: Multistage optoelectronic two-dimensional image switches

NASA Astrophysics Data System (ADS)

Fedorov, V. B.

1994-06-01

The implementation principles and the feasibility of construction of high-throughput multistage optoelectronic switches, capable of transmitting data in the form of two-dimensional images along interconnected pairs of optical channels, are considered. Different ways of realising compact switches are proposed. They are based on the use of polarisation-sensitive elements, arrays of modulators of the plane of polarisation of light, arrays of objectives, and free-space optics. Optical systems of such switches can theoretically ensure that the resolution and optical losses in two-dimensional image transmission are limited only by diffraction. Estimates are obtained of the main maximum-performance parameters of the proposed optoelectronic image switches.

Colorectal Cancer

MedlinePlus

... be detected by optical colonoscopy. Virtual colonoscopy uses virtual reality technology to produce three-dimensional images of the colon and rectum. However, the costs and benefits of virtual colonoscopy are still being investigated, and the technique ...

Label-free imaging of the dynamics of cell-to-cell string-like structure bridging in the free-space by low-coherent quantitative phase microscopy

NASA Astrophysics Data System (ADS)

Yamauchi, Toyohiko; Iwai, Hidenao; Yamashita, Yutaka

2013-03-01

We succeeded in utilizing our low-coherent quantitative phase microscopy (LC-QPM) to achieve label-free and three-dimensional imaging of string-like structures bridging the free-space between live cells. In past studies, three dimensional morphology of the string-like structures between cells had been investigated by electron microscopies and fluorescence microscopies and these structures were called "membrane nanotubes" or "tunneling nanotubes." However, use of electron microscopy inevitably kills these cells and fluorescence microscopy is itself a potentially invasive method. To achieve noninvasive imaging of live cells, we applied our LC-QPM which is a reflection-type, phase resolved and full-field interference microscope employing a low-coherent light source. LC-QPM is able to visualize the three-dimensional morphology of live cells without labeling by means of low-coherence interferometry. The lateral (diffraction limit) and longitudinal (coherence-length) spatial resolution of LC-QPM were respectively 0.49 and 0.93 micrometers and the repeatability of the phase measurement was 0.02 radians (1.0 nm). We successfully obtained three-dimensional morphology of live cultured epithelial cells (cell type: HeLa, derived from cervix cancer) and were able to clearly observe the individual string-like structures interconnecting the cells. When we performed volumetric imaging, a 80 micrometer by 60 micrometer by 6.5 micrometer volume was scanned every 5.67 seconds and 70 frames of a three-dimensional movie were recorded for a duration of 397 seconds. Moreover, the optical phase images gave us detailed information about the three-dimensional morphology of the string-like structure at sub-wavelength resolution. We believe that our LC-QPM will be a useful tool for the study of three-dimensional morphology of live cells.

In vivo three-dimensional imaging of human corneal nerves using Fourier-domain optical coherence tomography

NASA Astrophysics Data System (ADS)

Shin, Jun Geun; Hwang, Ho Sik; Eom, Tae Joong; Lee, Byeong Ha

2017-01-01

We have employed Fourier-domain optical coherence tomography (FD-OCT) to achieve corneal nerve imaging, which could be useful in surgical planning and refractive surgery. Because the three-dimensional (3-D) images of the corneal nerves were acquired in vivo, unintentional movement of the subject during the measurement led to imaging artifacts. These artifacts were compensated for with a series of signal processing techniques, namely realigning A-scan images to flatten the boundary and cross-correlating adjacent B-scan images. To overcome the undesirably large signal from scattering at the corneal surface and iris, volume rendering and maximum intensity projections were performed with only the data taken in the stromal region of the cornea, which is located between 200 and 500 μm from the corneal surface. The 3-D volume imaging of a 10×10 mm2 area took 9.8 s, which is slightly shorter than the normal tear breakup time. This allowed us to image the branched and threadlike corneal nerve bundles within the human eye. The experimental results show that FD-OCT systems have the potential to be useful in clinical investigations of corneal nerves and by minimizing nerve injury during clinical or surgical procedures.

Nodal aberration theory applied to freeform surfaces

NASA Astrophysics Data System (ADS)

Fuerschbach, Kyle; Rolland, Jannick P.; Thompson, Kevin P.

2014-12-01

When new three-dimensional packages are developed for imaging optical systems, the rotational symmetry of the optical system is often broken, changing its imaging behavior and making the optical performance worse. A method to restore the performance is to use freeform optical surfaces that compensate directly the aberrations introduced from tilting and decentering the optical surfaces. In order to effectively optimize the shape of a freeform surface to restore optical functionality, it is helpful to understand the aberration effect the surface may induce. Using nodal aberration theory the aberration fields induced by a freeform surface in an optical system are explored. These theoretical predications are experimentally validated with the design and implementation of an aberration generating telescope.

Diffuse optical tomography and spectroscopy of breast cancer and fetal brain

NASA Astrophysics Data System (ADS)

Choe, Regine

Diffuse optical techniques utilize light in the near infrared spectral range to measure tissue physiology non-invasively. Based on these measurements, either on average or a three-dimensional spatial map of tissue properties such as total hemoglobin concentration, blood oxygen saturation and scattering can be obtained using model-based reconstruction algorithms. In this thesis, diffuse optical techniques were applied for in vivo breast cancer imaging and trans-abdominal fetal brain oxygenation monitoring. For in vivo breast cancer imaging, clinical diffuse optical tomography and related instrumentation was developed and used in several contexts. Bulk physiological properties were quantified for fifty-two healthy subjects in the parallel-plate transmission geometry. Three-dimensional images of breast were reconstructed for subjects with breast tumors and, tumor contrast with respect to normal tissue was found in total hemoglobin concentration and scattering and was quantified for twenty-two breast carcinomas. Tumor contrast and tumor volume changes during neoadjuvant chemotherapy were tracked for one subject and compared to the dynamic contrast-enhanced MRI. Finally, the feasibility for measuring blood flow of breast tumors using optical methods was demonstrated for seven subjects. In a qualitatively different set of experiments, the feasibility for trans-abdominal fetal brain oxygenation monitoring was demonstrated on pregnant ewes with induced fetal hypoxia. Preliminary clinical experiences were discussed to identify future directions. In total, this research has translated diffuse optical tomography techniques into clinical research environment.

Four dimensional hybrid ultrasound and optoacoustic imaging via passive element optical excitation in a hand-held probe

NASA Astrophysics Data System (ADS)

Fehm, Thomas Felix; Deán-Ben, Xosé Luís; Razansky, Daniel

2014-10-01

Ultrasonography and optoacoustic imaging share powerful advantages related to the natural aptitude for real-time image rendering with high resolution, the hand-held operation, and lack of ionizing radiation. The two methods also possess very different yet highly complementary advantages of the mechanical and optical contrast in living tissues. Nonetheless, efficient integration of these modalities remains challenging owing to the fundamental differences in the underlying physical contrast, optimal signal acquisition, and image reconstruction approaches. We report on a method for hybrid acquisition and reconstruction of three-dimensional pulse-echo ultrasound and optoacoustic images in real time based on passive ultrasound generation with an optical absorber, thus avoiding the hardware complexity of active ultrasound generation. In this way, complete hybrid datasets are generated with a single laser interrogation pulse, resulting in simultaneous rendering of ultrasound and optoacoustic images at an unprecedented rate of 10 volumetric frames per second. Performance is subsequently showcased in phantom experiments and in-vivo measurements from a healthy human volunteer, confirming general clinical applicability of the method.

Projection-type see-through holographic three-dimensional display

NASA Astrophysics Data System (ADS)

Wakunami, Koki; Hsieh, Po-Yuan; Oi, Ryutaro; Senoh, Takanori; Sasaki, Hisayuki; Ichihashi, Yasuyuki; Okui, Makoto; Huang, Yi-Pai; Yamamoto, Kenji

2016-10-01

Owing to the limited spatio-temporal resolution of display devices, dynamic holographic three-dimensional displays suffer from a critical trade-off between the display size and the visual angle. Here we show a projection-type holographic three-dimensional display, in which a digitally designed holographic optical element and a digital holographic projection technique are combined to increase both factors at the same time. In the experiment, the enlarged holographic image, which is twice as large as the original display device, projected on the screen of the digitally designed holographic optical element was concentrated at the target observation area so as to increase the visual angle, which is six times as large as that for a general holographic display. Because the display size and the visual angle can be designed independently, the proposed system will accelerate the adoption of holographic three-dimensional displays in industrial applications, such as digital signage, in-car head-up displays, smart-glasses and head-mounted displays.

Solar tomography adaptive optics.

PubMed

Ren, Deqing; Zhu, Yongtian; Zhang, Xi; Dou, Jiangpei; Zhao, Gang

2014-03-10

Conventional solar adaptive optics uses one deformable mirror (DM) and one guide star for wave-front sensing, which seriously limits high-resolution imaging over a large field of view (FOV). Recent progress toward multiconjugate adaptive optics indicates that atmosphere turbulence induced wave-front distortion at different altitudes can be reconstructed by using multiple guide stars. To maximize the performance over a large FOV, we propose a solar tomography adaptive optics (TAO) system that uses tomographic wave-front information and uses one DM. We show that by fully taking advantage of the knowledge of three-dimensional wave-front distribution, a classical solar adaptive optics with one DM can provide an extra performance gain for high-resolution imaging over a large FOV in the near infrared. The TAO will allow existing one-deformable-mirror solar adaptive optics to deliver better performance over a large FOV for high-resolution magnetic field investigation, where solar activities occur in a two-dimensional field up to 60'', and where the near infrared is superior to the visible in terms of magnetic field sensitivity.

Automated sea floor extraction from underwater video

NASA Astrophysics Data System (ADS)

Kelly, Lauren; Rahmes, Mark; Stiver, James; McCluskey, Mike

2016-05-01

Ocean floor mapping using video is a method to simply and cost-effectively record large areas of the seafloor. Obtaining visual and elevation models has noteworthy applications in search and recovery missions. Hazards to navigation are abundant and pose a significant threat to the safety, effectiveness, and speed of naval operations and commercial vessels. This project's objective was to develop a workflow to automatically extract metadata from marine video and create image optical and elevation surface mosaics. Three developments made this possible. First, optical character recognition (OCR) by means of two-dimensional correlation, using a known character set, allowed for the capture of metadata from image files. Second, exploiting the image metadata (i.e., latitude, longitude, heading, camera angle, and depth readings) allowed for the determination of location and orientation of the image frame in mosaic. Image registration improved the accuracy of mosaicking. Finally, overlapping data allowed us to determine height information. A disparity map was created using the parallax from overlapping viewpoints of a given area and the relative height data was utilized to create a three-dimensional, textured elevation map.

Microvascular anastomosis guidance and evaluation using real-time three-dimensional Fourier-domain Doppler optical coherence tomography

PubMed Central

Ibrahim, Zuhaib; Tong, Dedi; Zhu, Shan; Mao, Qi; Pang, John; Andrew Lee, Wei Ping; Brandacher, Gerald; Kang, Jin U.

2013-01-01

Abstract. Vascular and microvascular anastomoses are critical components of reconstructive microsurgery, vascular surgery, and transplant surgery. Intraoperative surgical guidance using a surgical imaging modality that provides an in-depth view and three-dimensional (3-D) imaging can potentially improve outcome following both conventional and innovative anastomosis techniques. Objective postoperative imaging of the anastomosed vessel can potentially improve the salvage rate when combined with other clinical assessment tools, such as capillary refill, temperature, blanching, and skin turgor. Compared to other contemporary postoperative monitoring modalities—computed tomography angiograms, magnetic resonance (MR) angiograms, and ultrasound Doppler—optical coherence tomography (OCT) is a noninvasive high-resolution (micron-level), high-speed, 3-D imaging modality that has been adopted widely in biomedical and clinical applications. For the first time, to the best of our knowledge, the feasibility of real-time 3-D phase-resolved Doppler OCT (PRDOCT) as an assisted intra- and postoperative imaging modality for microvascular anastomosis of rodent femoral vessels is demonstrated, which will provide new insights and a potential breakthrough to microvascular and supermicrovascular surgery. PMID:23856833

Computational high-resolution optical imaging of the living human retina

NASA Astrophysics Data System (ADS)

Shemonski, Nathan D.; South, Fredrick A.; Liu, Yuan-Zhi; Adie, Steven G.; Scott Carney, P.; Boppart, Stephen A.

2015-07-01

High-resolution in vivo imaging is of great importance for the fields of biology and medicine. The introduction of hardware-based adaptive optics (HAO) has pushed the limits of optical imaging, enabling high-resolution near diffraction-limited imaging of previously unresolvable structures. In ophthalmology, when combined with optical coherence tomography, HAO has enabled a detailed three-dimensional visualization of photoreceptor distributions and individual nerve fibre bundles in the living human retina. However, the introduction of HAO hardware and supporting software adds considerable complexity and cost to an imaging system, limiting the number of researchers and medical professionals who could benefit from the technology. Here we demonstrate a fully automated computational approach that enables high-resolution in vivo ophthalmic imaging without the need for HAO. The results demonstrate that computational methods in coherent microscopy are applicable in highly dynamic living systems.

Endoscopes with latest technology and concept.

PubMed

Gotoh

2003-09-01

Endoscopic imaging systems that perform as the "eye" of the operator during endoscopic surgical procedures have developed rapidly due to various technological developments. In addition, since the most recent turn of the century robotic surgery has increased its scope through the utilization of systems such as Intuitive Surgical's da Vinci System. To optimize the imaging required for precise robotic surgery, a unique endoscope has been developed, consisting of both a two dimensional (2D) image optical system for wider observation of the entire surgical field, and a three dimensional (3D) image optical system for observation of the more precise details at the operative site. Additionally, a "near infrared radiation" endoscopic system is under development to detect the sentinel lymph node more readily. Such progress in the area of endoscopic imaging is expected to enhance the surgical procedure from both the patient's and the surgeon's point of view.

The representation of visual depth perception based on the plenoptic function in the retina and its neural computation in visual cortex V1.

PubMed

Songnian, Zhao; Qi, Zou; Chang, Liu; Xuemin, Liu; Shousi, Sun; Jun, Qiu

2014-04-23

How it is possible to "faithfully" represent a three-dimensional stereoscopic scene using Cartesian coordinates on a plane, and how three-dimensional perceptions differ between an actual scene and an image of the same scene are questions that have not yet been explored in depth. They seem like commonplace phenomena, but in fact, they are important and difficult issues for visual information processing, neural computation, physics, psychology, cognitive psychology, and neuroscience. The results of this study show that the use of plenoptic (or all-optical) functions and their dual plane parameterizations can not only explain the nature of information processing from the retina to the primary visual cortex and, in particular, the characteristics of the visual pathway's optical system and its affine transformation, but they can also clarify the reason why the vanishing point and line exist in a visual image. In addition, they can better explain the reasons why a three-dimensional Cartesian coordinate system can be introduced into the two-dimensional plane to express a real three-dimensional scene. 1. We introduce two different mathematical expressions of the plenoptic functions, Pw and Pv that can describe the objective world. We also analyze the differences between these two functions when describing visual depth perception, that is, the difference between how these two functions obtain the depth information of an external scene.2. The main results include a basic method for introducing a three-dimensional Cartesian coordinate system into a two-dimensional plane to express the depth of a scene, its constraints, and algorithmic implementation. In particular, we include a method to separate the plenoptic function and proceed with the corresponding transformation in the retina and visual cortex.3. We propose that size constancy, the vanishing point, and vanishing line form the basis of visual perception of the outside world, and that the introduction of a three-dimensional Cartesian coordinate system into a two dimensional plane reveals a corresponding mapping between a retinal image and the vanishing point and line.

The representation of visual depth perception based on the plenoptic function in the retina and its neural computation in visual cortex V1

PubMed Central

2014-01-01

Background How it is possible to “faithfully” represent a three-dimensional stereoscopic scene using Cartesian coordinates on a plane, and how three-dimensional perceptions differ between an actual scene and an image of the same scene are questions that have not yet been explored in depth. They seem like commonplace phenomena, but in fact, they are important and difficult issues for visual information processing, neural computation, physics, psychology, cognitive psychology, and neuroscience. Results The results of this study show that the use of plenoptic (or all-optical) functions and their dual plane parameterizations can not only explain the nature of information processing from the retina to the primary visual cortex and, in particular, the characteristics of the visual pathway’s optical system and its affine transformation, but they can also clarify the reason why the vanishing point and line exist in a visual image. In addition, they can better explain the reasons why a three-dimensional Cartesian coordinate system can be introduced into the two-dimensional plane to express a real three-dimensional scene. Conclusions 1. We introduce two different mathematical expressions of the plenoptic functions, P w and P v that can describe the objective world. We also analyze the differences between these two functions when describing visual depth perception, that is, the difference between how these two functions obtain the depth information of an external scene. 2. The main results include a basic method for introducing a three-dimensional Cartesian coordinate system into a two-dimensional plane to express the depth of a scene, its constraints, and algorithmic implementation. In particular, we include a method to separate the plenoptic function and proceed with the corresponding transformation in the retina and visual cortex. 3. We propose that size constancy, the vanishing point, and vanishing line form the basis of visual perception of the outside world, and that the introduction of a three-dimensional Cartesian coordinate system into a two dimensional plane reveals a corresponding mapping between a retinal image and the vanishing point and line. PMID:24755246

Energy and Technology Review

NASA Astrophysics Data System (ADS)

Poggio, Andrew J.

1988-10-01

This issue of Energy and Technology Review contains: Neutron Penumbral Imaging of Laser-Fusion Targets--using our new penumbral-imaging diagnostic, we have obtained the first images that can be used to measure directly the deuterium-tritium burn region in laser-driven fusion targets; Computed Tomography for Nondestructive Evaluation--various computed tomography systems and computational techniques are used in nondestructive evaluation; Three-Dimensional Image Analysis for Studying Nuclear Chromatin Structure--we have developed an optic-electronic system for acquiring cross-sectional views of cell nuclei, and computer codes to analyze these images and reconstruct the three-dimensional structures they represent; Imaging in the Nuclear Test Program--advanced techniques produce images of unprecedented detail and resolution from Nevada Test Site data; and Computational X-Ray Holography--visible-light experiments and numerically simulated holograms test our ideas about an X-ray microscope for biological research.

Three-dimensional ghost imaging using acoustic transducer

NASA Astrophysics Data System (ADS)

Zhang, Chi; Guo, Shuxu; Guan, Jian; Cao, Junsheng; Gao, Fengli

2016-06-01

We propose a novel three-dimensional (3D) ghost imaging method using unfocused ultrasonic transducer, where the transducer is used as the bucket detector to collect the total photoacoustic signal intensity from spherical surfaces with different radius circling the transducer. This collected signal is a time sequence corresponding to the optic absorption information on the spherical surfaces, and the values at the same moments in all the sequences are used as the bucket signals to restore the corresponding spherical images, which are assembled as the object 3D reconstruction. Numerical experiments show this method can effectively accomplish the 3D reconstruction and by adding up each sequence on time domain as a bucket signal it can also realize two dimensional (2D) ghost imaging. The influence of the measurement times on the 3D and 2D reconstruction is analyzed with Peak Signal to Noise Ratio (PSNR) as the yardstick, and the transducer as a bucket detector is also discussed.

Generation of three-dimensional optical cusp beams with ultrathin metasurfaces.

PubMed

Liu, Weiwei; Zhang, Yuchao; Gao, Jie; Yang, Xiaodong

2018-06-22

Cusp beams are one type of complex structured beams with unique multiple self-accelerating channels and needle-like field structures owning great potentials to advance applications such as particle micromanipulation and super-resolution imaging. The traditional method to generate optical catastrophe is based on cumbrous reflective diffraction optical elements, which makes optical system complicated and hinders the nanophotonics integration. Here we design geometric phase based ultrathin plasmonic metasurfaces made of nanoslit antennas to produce three-dimensional (3D) optical cusp beams with variable numbers of self-accelerating channels in a broadband wavelength range. The entire beam propagation profiles of the cusp beams generated from the metasurfaces are mapped theoretically and experimentally. The special self-accelerating behavior and caustics concentration property of the cups beams are also demonstrated. Our results provide great potentials for promoting metasurface-enabled compact photonic devices used in wide applications of light-matter interactions.

Digital holography applications in ophthalmology, biometry, and optical trapping characterization

NASA Astrophysics Data System (ADS)

Potcoava, Mariana Camelia

This dissertation combines various holographic techniques with application on the two- and three-dimensional imaging of ophthalmic tissue, fingerprints, and microsphere samples with micrometer resolution. Digital interference holography (DIH) uses scanned wavelengths to synthesize short-coherence interference tomographic images. We used DIH for in vitro imaging of human optic nerve head and retina. Tomographic images were produced by superposition of holograms. Holograms were obtained with a signal-to-noise ratio of approximately 50 dB. Optic nerve head characteristics (shape, diameter, cup depth, and cup width) were quantified with a few micron resolution (4.06--4.8mum). Multiple layers were distinguishable in cross-sectional images of the macula. To our knowledge, this is the first report of DIH use to image human macular and optic nerve tissue. Holographic phase microscopy is used to produce images of thin film patterns left by latent fingerprints. Two or more holographic phase images with different wavelengths are combined for optical phase unwrapping of images of patent prints. We demonstrated digital interference holography images of a plastic print, and latent prints. These demonstrations point to significant contributions to biometry by using digital interference holography to identify and quantify Level 1 (pattern), Level 2 (minutia points), and Level 3 (pores and ridge contours). Quantitative studies of physical and biological processes and precise non-contact manipulation of nanometer/micrometer trapped objects can be effectuated with nanometer accuracy due to the development of optical tweezers. A three-dimensional gradient trap is produced at the focus position of a high NA microscope objective. Particles are trapped axially and laterally due to the gradient force. The particle is confined in a potential well and the trap acts as a harmonic spring. The elastic constant or the stiffness along any axis is determined from the particle displacements in time along each specific axis. Thus, we report the sensing of small particles using optical trapping in combination with the digital Gabor holography to calibrate the optical force and the position and of the copolymer microsphere in the x, y, z direction with nm precision.

Mass Storage and Retrieval at Rome Laboratory

NASA Technical Reports Server (NTRS)

Kann, Joshua L.; Canfield, Brady W.; Jamberdino, Albert A.; Clarke, Bernard J.; Daniszewski, Ed; Sunada, Gary

1996-01-01

As the speed and power of modern digital computers continues to advance, the demands on secondary mass storage systems grow. In many cases, the limitations of existing mass storage reduce the overall effectiveness of the computing system. Image storage and retrieval is one important area where improved storage technologies are required. Three dimensional optical memories offer the advantage of large data density, on the order of 1 Tb/cm(exp 3), and faster transfer rates because of the parallel nature of optical recording. Such a system allows for the storage of multiple-Gbit sized images, which can be recorded and accessed at reasonable rates. Rome Laboratory is currently investigating several techniques to perform three-dimensional optical storage including holographic recording, two-photon recording, persistent spectral-hole burning, multi-wavelength DNA recording, and the use of bacteriorhodopsin as a recording material. In this paper, the current status of each of these on-going efforts is discussed. In particular, the potential payoffs as well as possible limitations are addressed.

Method for producing three-dimensional real image using radiographic perspective views of an object

DOEpatents

Ellingson, William A.; Read, Alvin A.

1976-02-24

A sequence of separate radiographs are made by indexing a radiation source along a known path relative to the object under study. Thus, each radiograph contains information from a different perspective. A holographically-recorded image is then made from each radiographic perspective by exact re-tracing of the rays through each radiographic perspective such that the re-tracing duplicates the geometry under which it was originally prepared. The holographically-stored images are simultaneously illuminated with the conjugate of the reference beam used in the original recordings. The result is the generation of a three-dimensional real image of the object such that a light-sensitive device can be moved to view the real image along any desired surface with the optical information in all other surfaces greatly suppressed.

A dual-modality optical coherence tomography and selective plane illumination microscopy system for mouse embryonic imaging

NASA Astrophysics Data System (ADS)

Wu, Chen; Ran, Shihao; Le, Henry; Singh, Manmohan; Larina, Irina V.; Mayerich, David; Dickinson, Mary E.; Larin, Kirill V.

2017-02-01

Both optical coherence tomography (OCT) and selective plane illumination microscopy (SPIM) are frequently used in mouse embryonic research for high-resolution three-dimensional imaging. However, each of these imaging methods provide a unique and independent advantage: SPIM provides morpho-functional information through immunofluorescence and OCT provides a method for whole-embryo 3D imaging. In this study, we have combined rotational imaging OCT and SPIM into a single, dual-modality device to image E9.5 mouse embryos. The results demonstrate that the dual-modality setup is able to provide both anatomical and functional information simultaneously for more comprehensive tissue characterization.

Three-dimensional displays and stereo vision

PubMed Central

Westheimer, Gerald

2011-01-01

Procedures for three-dimensional image reconstruction that are based on the optical and neural apparatus of human stereoscopic vision have to be designed to work in conjunction with it. The principal methods of implementing stereo displays are described. Properties of the human visual system are outlined as they relate to depth discrimination capabilities and achieving optimal performance in stereo tasks. The concept of depth rendition is introduced to define the change in the parameters of three-dimensional configurations for cases in which the physical disposition of the stereo camera with respect to the viewed object differs from that of the observer's eyes. PMID:21490023

In vivo three-dimensional photoacoustic imaging of the renal vasculature in preclinical rodent models.

PubMed

Ogunlade, Olumide; Connell, John J; Huang, Jennifer L; Zhang, Edward; Lythgoe, Mark F; Long, David A; Beard, Paul

2018-06-01

Noninvasive imaging of the kidney vasculature in preclinical murine models is important for the assessment of renal development, studying diseases and evaluating new therapies but is challenging to achieve using existing imaging modalities. Photoacoustic imaging is a promising new technique that is particularly well suited to visualizing the vasculature and could provide an alternative to existing preclinical imaging methods for studying renal vascular anatomy and function. To investigate this, an all-optical Fabry-Perot-based photoacoustic scanner was used to image the abdominal region of mice. High-resolution three-dimensional, noninvasive, label-free photoacoustic images of the mouse kidney and renal vasculature were acquired in vivo. The scanner was also used to visualize and quantify differences in the vascular architecture of the kidney in vivo due to polycystic kidney disease. This study suggests that photoacoustic imaging could be utilized as a novel preclinical imaging tool for studying the biology of renal disease.

Confocal Imaging of porous media

NASA Astrophysics Data System (ADS)

Shah, S.; Crawshaw, D.; Boek, D.

2012-12-01

Carbonate rocks, which hold approximately 50% of the world's oil and gas reserves, have a very complicated and heterogeneous structure in comparison with sandstone reservoir rock. We present advances with different techniques to image, reconstruct, and characterize statistically the micro-geometry of carbonate pores. The main goal here is to develop a technique to obtain two dimensional and three dimensional images using Confocal Laser Scanning Microscopy. CLSM is used in epi-fluorescent imaging mode, allowing for the very high optical resolution of features well below 1μm size. Images of pore structures were captured using CLSM imaging where spaces in the carbonate samples were impregnated with a fluorescent, dyed epoxy-resin, and scanned in the x-y plane by a laser probe. We discuss the sample preparation in detail for Confocal Imaging to obtain sub-micron resolution images of heterogeneous carbonate rocks. We also discuss the technical and practical aspects of this imaging technique, including its advantages and limitation. We present several examples of this application, including studying pore geometry in carbonates, characterizing sub-resolution porosity in two dimensional images. We then describe approaches to extract statistical information about porosity using image processing and spatial correlation function. We have managed to obtain very low depth information in z -axis (~ 50μm) to develop three dimensional images of carbonate rocks with the current capabilities and limitation of CLSM technique. Hence, we have planned a novel technique to obtain higher depth information to obtain high three dimensional images with sub-micron resolution possible in the lateral and axial planes.

Transmission mode terahertz computed tomography

DOEpatents

Ferguson, Bradley Stuart; Wang, Shaohong; Zhang, Xi-Cheng

2006-10-10

A method of obtaining a series of images of a three-dimensional object by transmitting pulsed terahertz (THz) radiation through the entire object from a plurality of angles, optically detecting changes in the transmitted THz radiation using pulsed laser radiation, and constructing a plurality of imaged slices of the three-dimensional object using the detected changes in the transmitted THz radiation. The THz radiation is transmitted through the object as a scanning spot. The object is placed within the Rayleigh range of the focused THz beam and a focusing system is used to transfer the imaging plane from adjacent the object to a desired distance away from the object. A related system is also disclosed.

Optical rotation compensation for a holographic 3D display with a 360 degree horizontal viewing zone.

PubMed

Sando, Yusuke; Barada, Daisuke; Yatagai, Toyohiko

2016-10-20

A method for a continuous optical rotation compensation in a time-division-based holographic three-dimensional (3D) display with a rotating mirror is presented. Since the coordinate system of wavefronts after the mirror reflection rotates about the optical axis along with the rotation angle, compensation or cancellation is absolutely necessary to fix the reconstructed 3D object. In this study, we address this problem by introducing an optical image rotator based on a right-angle prism that rotates synchronously with the rotating mirror. The optical and continuous compensation reduces the occurrence of duplicate images, which leads to the improvement of the quality of reconstructed images. The effect of the optical rotation compensation is experimentally verified and a demonstration of holographic 3D display with the optical rotation compensation is presented.

Dual-color three-dimensional STED microscopy with a single high-repetition-rate laser

PubMed Central

Han, Kyu Young; Ha, Taekjip

2016-01-01

We describe a dual-color three-dimensional stimulated emission depletion (3D-STED) microscopy employing a single laser source with a repetition rate of 80 MHz. Multiple excitation pulses synchronized with a STED pulse were generated by a photonic crystal fiber and the desired wavelengths were selected by an acousto-optic tunable filter with high spectral purity. Selective excitation at different wavelengths permits simultaneous imaging of two fluorescent markers at a nanoscale resolution in three dimensions. PMID:26030581

Expanding the limits of endoscopic intraorbital tumor resection using 3-dimensional reconstruction.

PubMed

Gregorio, Luciano Lobato; Busaba, Nicolas Y; Miyake, Marcel M; Freitag, Suzanne K; Bleier, Benjamin S

2017-12-26

Endoscopic orbital surgery is a nascent field and new tools are required to assist with surgical planning and to ascertain the limits of the tumor resectability. We purpose to utilize three-dimensional radiographic reconstruction to define the theoretical lateral limit of endoscopic resectability of primary orbital tumors and to apply these boundary conditions to surgical cases. A three-dimensional orbital model was rendered in 4 representative patients presenting with primary orbital tumors using OsiriX open source imaging software. A 2-Dimensional plane was propagated between the contralateral nare and a line tangential to the long axis of the optic nerve reflecting the trajectory of a trans-septal approach. Any tumor volume falling medial to the optic nerve and/or within the space inferior to this plane of resectability was considered theoretically resectable regardless of how far it extended lateral to the optic nerve as nerve retraction would be unnecessary. Actual tumor volumes were then superimposed over this plan and correlated with surgical outcomes. Among the 4 lesions analyzed, two were fully medial to the optic nerve, one extended lateral to the optic nerve but remained inferior to the plane of resectability, and one extended both lateral to the optic nerve and superior to the plane of resectability. As predicted by the three-dimensional modeling, a complete resection was achieved in all lesions except one that transgressed the plane of resectability. No new diplopia or vision loss was observed in any patient. Three-dimensional reconstruction enhances preoperative planning for endoscopic orbital surgery. Tumors that extend lateral to the optic nerve may still be candidates for a purely endoscopic resection as long as they do not extend above the plane of resectability described herein. Copyright © 2017 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial. Published by Elsevier Editora Ltda. All rights reserved.

Time-Resolved and Spectroscopic Three-Dimensional Optical Breast Tomography

DTIC Science & Technology

2009-03-01

polarization sensitive imaging 16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF ABSTRACT 18. NUMBER OF PAGES 19a. NAME OF RESPONSIBLE PERSON R. R...project; • Development of a near-infrared center of intensity time gated imaging approach; and • Polarization sensitive imaging. We provide an...spectroscopic imaging arrangement, and a multi-source illumination and multi- detector signal acquisition arrangement. 5 5.1.1. Time-resolved transillumination

Visible/Infrared Optical Depths of Cirrus as Seen by Satellite and Scanning Lidar

NASA Technical Reports Server (NTRS)

Wylie, Donald; Wolf, Walt; Piironen, Paivi; Eloranta, Edwin

1996-01-01

The High Spectral Resolution Lidar (HSRL) and the Volume Imaging Lidar (VIL) were combined to produce a quantitative image of the visible optical depth of cirrus clouds. The HSRL was used to calibrate the VIL signal into backscatter cross sections of particulates. The backscatter cross sections were related to extinction by a constant backscatter phase function determined from the HSRL data. This produced a three dimensional image of visual extinction in the cirrus clouds over a one hour period. Two lidar images were constructed from one hour VIL cross section records.

Investigating line- versus point-laser excitation for three-dimensional fluorescence imaging and tomography employing a trimodal imaging system

NASA Astrophysics Data System (ADS)

Cao, Liji; Peter, Jörg

2013-06-01

The adoption of axially oriented line illumination patterns for fluorescence excitation in small animals for fluorescence surface imaging (FSI) and fluorescence optical tomography (FOT) is being investigated. A trimodal single-photon-emission-computed-tomography/computed-tomography/optical-tomography (SPECT-CT-OT) small animal imaging system is being modified for employment of point- and line-laser excitation sources. These sources can be arbitrarily positioned around the imaged object. The line source is set to illuminate the object along its entire axial direction. Comparative evaluation of point and line illumination patterns for FSI and FOT is provided involving phantom as well as mouse data. Given the trimodal setup, CT data are used to guide the optical approaches by providing boundary information. Furthermore, FOT results are also being compared to SPECT. Results show that line-laser illumination yields a larger axial field of view (FOV) in FSI mode, hence faster data acquisition, and practically acceptable FOT reconstruction throughout the whole animal. Also, superimposed SPECT and FOT data provide additional information on similarities as well as differences in the distribution and uptake of both probe types. Fused CT data enhance further the anatomical localization of the tracer distribution in vivo. The feasibility of line-laser excitation for three-dimensional fluorescence imaging and tomography is demonstrated for initiating further research, however, not with the intention to replace one by the other.

Adaptive optics optical coherence tomography with dynamic retinal tracking

PubMed Central

Kocaoglu, Omer P.; Ferguson, R. Daniel; Jonnal, Ravi S.; Liu, Zhuolin; Wang, Qiang; Hammer, Daniel X.; Miller, Donald T.

2014-01-01

Adaptive optics optical coherence tomography (AO-OCT) is a highly sensitive and noninvasive method for three dimensional imaging of the microscopic retina. Like all in vivo retinal imaging techniques, however, it suffers the effects of involuntary eye movements that occur even under normal fixation. In this study we investigated dynamic retinal tracking to measure and correct eye motion at KHz rates for AO-OCT imaging. A customized retina tracking module was integrated into the sample arm of the 2nd-generation Indiana AO-OCT system and images were acquired on three subjects. Analyses were developed based on temporal amplitude and spatial power spectra in conjunction with strip-wise registration to independently measure AO-OCT tracking performance. After optimization of the tracker parameters, the system was found to correct eye movements up to 100 Hz and reduce residual motion to 10 µm root mean square. Between session precision was 33 µm. Performance was limited by tracker-generated noise at high temporal frequencies. PMID:25071963

Efficient analysis of three dimensional EUV mask induced imaging artifacts using the waveguide decomposition method

NASA Astrophysics Data System (ADS)

Shao, Feng; Evanschitzky, Peter; Fühner, Tim; Erdmann, Andreas

2009-10-01

This paper employs the Waveguide decomposition method as an efficient rigorous electromagnetic field (EMF) solver to investigate three dimensional mask-induced imaging artifacts in EUV lithography. The major mask diffraction induced imaging artifacts are first identified by applying the Zernike analysis of the mask nearfield spectrum of 2D lines/spaces. Three dimensional mask features like 22nm semidense/dense contacts/posts, isolated elbows and line-ends are then investigated in terms of lithographic results. After that, the 3D mask-induced imaging artifacts such as feature orientation dependent best focus shift, process window asymmetries, and other aberration-like phenomena are explored for the studied mask features. The simulation results can help lithographers to understand the reasons of EUV-specific imaging artifacts and to devise illumination and feature dependent strategies for their compensation in the optical proximity correction (OPC) for EUV masks. At last, an efficient approach using the Zernike analysis together with the Waveguide decomposition technique is proposed to characterize the impact of mask properties for the future OPC process.

Quantitative Evaluation of Adult Subglottic Stenosis Using Intraoperative Long-range Optical Coherence Tomography

PubMed Central

Sharma, Giriraj K.; Loy, Anthony Chin; Su, Erica; Jing, Joe; Chen, Zhongping; Wong, Brian J-F.; Verma, Sunil

2016-01-01

Objectives To determine the feasibility of long-range optical coherence tomography (LR-OCT) as a tool to intraoperatively image and measure the subglottis and trachea during suspension microlaryngoscopy before and after endoscopic treatment of subglottic stenosis (SGS). Methods Long-range optical coherence tomography of the adult subglottis and trachea was performed during suspension microlaryngoscopy before and after endoscopic treatment for SGS. The anteroposterior and transverse diameters, cross-sectional area (CSA), distance from the vocal cords, and length of the SGS were measured using a MATLAB software. Pre-intervention and postintervention airway dimensions were compared. Three-dimensional volumetric airway reconstructions were generated using medical image processing software (MIMICS). Results Intraoperative LR-OCT imaging was performed in 3 patients undergoing endoscopic management of SGS. Statistically significant differences in mean anteroposterior diameter (P < .01), transverse diameter (P < .001), and CSA (P < .001) were noted between pre-intervention and postintervention data. Three-dimensional airway models were viewed in cross-sectional format and via virtual “fly through” bronchoscopy. Conclusions This is the first report of intraoperative LR-OCT of the subglottic and tracheal airway before and after surgical management of SGS in humans. Long-range optical coherence tomography offers a practical means to measure the dimensions of SGS and acquire objective data on the response to endoscopic treatment of SGS. PMID:27354215

High-contrast fast Fourier transform acousto-optical tomography of phantom tissues with a frequency-chirp modulation of the ultrasound.

PubMed

Forget, Benoît-Claude; Ramaz, François; Atlan, Michaël; Selb, Juliette; Boccara, Albert-Claude

2003-03-01

We report new results on acousto-optical tomography in phantom tissues using a frequency chirp modulation and a CCD camera. This technique allows quick recording of three-dimensional images of the optical contrast with a two-dimensional scan of the ultrasound source in a plane perpendicular to the ultrasonic path. The entire optical contrast along the ultrasonic path is concurrently obtained from the capture of a film sequence at a rate of 200 Hz. This technique reduces the acquisition time, and it enhances the axial resolution and thus the contrast, which are usually poor owing to the large volume of interaction of the ultrasound perturbation.

Three-dimensional image study on the vascular structure after angiopoietin-1 transduction in isolated mouse pancreatic islets

NASA Astrophysics Data System (ADS)

He, Jing; Su, Dongming; Trucco, Massimo

2008-02-01

Angiopoietin-1 (Ang-1) is essential for remodeling the primitive vascular plexus during embryonic development and for reducing plasma leakage in inflammation of adult vasculature. However, the role for Ang-1 in maintenance of vascular stability in isolated pancreatic islets is not fully understood. In this study, we compared the difference of vascular morphology between Ang-1 treated (n=5) and control mouse islets (n=5) using both two- and three-dimensional optical image analysis. Isolated mouse islets were transduced with Ang-1 or Lac Z (control) vector at 37°C for 16 hours. Islets were incubated with both rat anti-CD31 antibody and rabbit anti-insulin antibody followed by incubation with Rhodamine-conjugated goat anti-rat IgG and Alexa-488 conjugated goat anti-rabbit IgG. Islets were viewed under a Nikon confocal microscope. Serial optical section images were captured and reconstructed using Nikon EZ-C1 software. Individual two-D and reconstructed three-D images were analyzed using MetaMorph Image Analysis software. Islet vascular density was determined. In two-D images, there was no significant difference of vascular density between the two groups. The vascular morphology didn't show any obvious differences in two-D images either. However, in the three-D images, we found higher vascular density and more vascular branches in the Ang-1 transducted islets and vascular dilation in control group. In conclusion, using three-D image analysis, Ang-1 displayed functions in maintenance of vascular stability and in stimulating growth of vascular branches in isolated mouse pancreatic islets. In order to study further the regeneration of different cell contents in the spherical pancreatic islet, three-D image analysis is an effective method to approach this goal.

Three-dimensional registration of intravascular optical coherence tomography and cryo-image volumes for microscopic-resolution validation.

PubMed

Prabhu, David; Mehanna, Emile; Gargesha, Madhusudhana; Brandt, Eric; Wen, Di; van Ditzhuijzen, Nienke S; Chamie, Daniel; Yamamoto, Hirosada; Fujino, Yusuke; Alian, Ali; Patel, Jaymin; Costa, Marco; Bezerra, Hiram G; Wilson, David L

2016-04-01

Evidence suggests high-resolution, high-contrast, [Formula: see text] intravascular optical coherence tomography (IVOCT) can distinguish plaque types, but further validation is needed, especially for automated plaque characterization. We developed experimental and three-dimensional (3-D) registration methods to provide validation of IVOCT pullback volumes using microscopic, color, and fluorescent cryo-image volumes with optional registered cryo-histology. A specialized registration method matched IVOCT pullback images acquired in the catheter reference frame to a true 3-D cryo-image volume. Briefly, an 11-parameter registration model including a polynomial virtual catheter was initialized within the cryo-image volume, and perpendicular images were extracted, mimicking IVOCT image acquisition. Virtual catheter parameters were optimized to maximize cryo and IVOCT lumen overlap. Multiple assessments suggested that the registration error was better than the [Formula: see text] spacing between IVOCT image frames. Tests on a digital synthetic phantom gave a registration error of only [Formula: see text] (signed distance). Visual assessment of randomly presented nearby frames suggested registration accuracy within 1 IVOCT frame interval ([Formula: see text]). This would eliminate potential misinterpretations confronted by the typical histological approaches to validation, with estimated 1-mm errors. The method can be used to create annotated datasets and automated plaque classification methods and can be extended to other intravascular imaging modalities.

Implementation of spatial overlap modulation nonlinear optical microscopy using an electro-optic deflector

PubMed Central

Isobe, Keisuke; Kawano, Hiroyuki; Kumagai, Akiko; Miyawaki, Atsushi; Midorikawa, Katsumi

2013-01-01

A spatial overlap modulation (SPOM) technique is a nonlinear optical microscopy technique which enhances the three-dimensional spatial resolution and rejects the out-of-focus background limiting the imaging depth inside a highly scattering sample. Here, we report on the implementation of SPOM in which beam pointing modulation is achieved by an electro-optic deflector. The modulation and demodulation frequencies are enhanced to 200 kHz and 400 kHz, respectively, resulting in a 200-fold enhancement compared with the previously reported system. The resolution enhancement and suppression of the out-of-focus background are demonstrated by sum-frequency-generation imaging of pounded granulated sugar and deep imaging of fluorescent beads in a tissue-like phantom, respectively. PMID:24156055

Three-dimensional morphological imaging of human induced pluripotent stem cells by using low-coherence quantitative phase microscopy

NASA Astrophysics Data System (ADS)

Yamauchi, Toyohiko; Kakuno, Yumi; Goto, Kentaro; Fukami, Tadashi; Sugiyama, Norikazu; Iwai, Hidenao; Mizuguchi, Yoshinori; Yamashita, Yutaka

2014-03-01

There is an increasing need for non-invasive imaging techniques in the field of stem cell research. Label-free techniques are the best choice for assessment of stem cells because the cells remain intact after imaging and can be used for further studies such as differentiation induction. To develop a high-resolution label-free imaging system, we have been working on a low-coherence quantitative phase microscope (LC-QPM). LC-QPM is a Linnik-type interference microscope equipped with nanometer-resolution optical-path-length control and capable of obtaining three-dimensional volumetric images. The lateral and vertical resolutions of our system are respectively 0.5 and 0.93 μm and this performance allows capturing sub-cellular morphological features of live cells without labeling. Utilizing LC-QPM, we reported on three-dimensional imaging of membrane fluctuations, dynamics of filopodia, and motions of intracellular organelles. In this presentation, we report three-dimensional morphological imaging of human induced pluripotent stem cells (hiPS cells). Two groups of monolayer hiPS cell cultures were prepared so that one group was cultured in a suitable culture medium that kept the cells undifferentiated, and the other group was cultured in a medium supplemented with retinoic acid, which forces the stem cells to differentiate. The volumetric images of the 2 groups show distinctive differences, especially in surface roughness. We believe that our LC-QPM system will prove useful in assessing many other stem cell conditions.

Three-dimensional multispectral optoacoustic mesoscopy reveals melanin and blood oxygenation in human skin in vivo.

PubMed

Schwarz, Mathias; Buehler, Andreas; Aguirre, Juan; Ntziachristos, Vasilis

2016-01-01

Optical imaging plays a major role in disease detection in dermatology. However, current optical methods are limited by lack of three-dimensional detection of pathophysiological parameters within skin. It was recently shown that single-wavelength optoacoustic (photoacoustic) mesoscopy resolves skin morphology, i.e. melanin and blood vessels within epidermis and dermis. In this work we employed illumination at multiple wavelengths for enabling three-dimensional multispectral optoacoustic mesoscopy (MSOM) of natural chromophores in human skin in vivo operating at 15-125 MHz. We employ a per-pulse tunable laser to inherently co-register spectral datasets, and reveal previously undisclosed insights of melanin, and blood oxygenation in human skin. We further reveal broadband absorption spectra of specific skin compartments. We discuss the potential of MSOM for label-free visualization of physiological biomarkers in skin in vivo. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Two-Photon Fluorescence Microscopy Developed for Microgravity Fluid Physics

NASA Technical Reports Server (NTRS)

Fischer, David G.; Zimmerli, Gregory A.; Asipauskas, Marius

2004-01-01

Recent research efforts within the Microgravity Fluid Physics Branch of the NASA Glenn Research Center have necessitated the development of a microscope capable of high-resolution, three-dimensional imaging of intracellular structure and tissue morphology. Standard optical microscopy works well for thin samples, but it does not allow the imaging of thick samples because of severe degradation caused by out-of-focus object structure. Confocal microscopy, which is a laser-based scanning microscopy, provides improved three-dimensional imaging and true optical sectioning by excluding the out-of-focus light. However, in confocal microscopy, out-of-focus object structure is still illuminated by the incoming beam, which can lead to substantial photo-bleaching. In addition, confocal microscopy is plagued by limited penetration depth, signal loss due to the presence of a confocal pinhole, and the possibility of live-cell damage. Two-photon microscopy is a novel form of laser-based scanning microscopy that allows three-dimensional imaging without many of the problems inherent in confocal microscopy. Unlike one-photon microscopy, it utilizes the nonlinear absorption of two near-infrared photons. However, the efficiency of two-photon absorption is much lower than that of one-photon absorption because of the nonlinear (i.e., quadratic) electric field dependence, so an ultrafast pulsed laser source must typically be employed. On the other hand, this stringent energy density requirement effectively localizes fluorophore excitation to the focal volume. Consequently, two-photon microscopy provides optical sectioning and confocal performance without the need for a signal-limiting pinhole. In addition, there is a reduction in photo-damage because of the longer excitation wavelength, a reduction in background fluorescence, and a 4 increase in penetration depth over confocal methods because of the reduction in Rayleigh scattering.

Independent synchronized control and visualization of interactions between living cells and organisms.

PubMed

Rouger, Vincent; Bordet, Guillaume; Couillault, Carole; Monneret, Serge; Mailfert, Sébastien; Ewbank, Jonathan J; Pujol, Nathalie; Marguet, Didier

2014-05-20

To investigate the early stages of cell-cell interactions occurring between living biological samples, imaging methods with appropriate spatiotemporal resolution are required. Among the techniques currently available, those based on optical trapping are promising. Methods to image trapped objects, however, in general suffer from a lack of three-dimensional resolution, due to technical constraints. Here, we have developed an original setup comprising two independent modules: holographic optical tweezers, which offer a versatile and precise way to move multiple objects simultaneously but independently, and a confocal microscope that provides fast three-dimensional image acquisition. The optical decoupling of these two modules through the same objective gives users the possibility to easily investigate very early steps in biological interactions. We illustrate the potential of this setup with an analysis of infection by the fungus Drechmeria coniospora of different developmental stages of Caenorhabditis elegans. This has allowed us to identify specific areas on the nematode's surface where fungal spores adhere preferentially. We also quantified this adhesion process for different mutant nematode strains, and thereby derive insights into the host factors that mediate fungal spore adhesion. Copyright © 2014 Biophysical Society. Published by Elsevier Inc. All rights reserved.

A hybrid scanning force and light microscope for surface imaging and three-dimensional optical sectioning in differential interference contrast.

PubMed

Stemmer, A

1995-04-01

The design of a scanned-cantilever-type force microscope is presented which is fully integrated into an inverted high-resolution video-enhanced light microscope. This set-up allows us to acquire thin optical sections in differential interference contrast (DIC) or polarization while the force microscope is in place. Such a hybrid microscope provides a unique platform to study how cell surface properties determine, or are affected by, the three-dimensional dynamic organization inside the living cell. The hybrid microscope presented in this paper has proven reliable and versatile for biological applications. It is the only instrument that can image a specimen by force microscopy and high-power DIC without having either to translate the specimen or to remove the force microscope. Adaptation of the design features could greatly enhance the suitability of other force microscopes for biological work.

Computational modeling of optical projection tomographic microscopy using the finite difference time domain method.

PubMed

Coe, Ryan L; Seibel, Eric J

2012-12-01

We present a method for modeling image formation in optical projection tomographic microscopy (OPTM) using high numerical aperture (NA) condensers and objectives. Similar to techniques used in computed tomography, OPTM produces three-dimensional, reconstructed images of single cells from two-dimensional projections. The model is capable of simulating axial scanning of a microscope objective to produce projections, which are reconstructed using filtered backprojection. Simulation of optical scattering in transmission optical microscopy is designed to analyze all aspects of OPTM image formation, such as degree of specimen staining, refractive-index matching, and objective scanning. In this preliminary work, a set of simulations is performed to examine the effect of changing the condenser NA, objective scan range, and complex refractive index on the final reconstruction of a microshell with an outer radius of 1.5 μm and an inner radius of 0.9 μm. The model lays the groundwork for optimizing OPTM imaging parameters and triaging efforts to further improve the overall system design. As the model is expanded in the future, it will be used to simulate a more realistic cell, which could lead to even greater impact.

Application of dot-matrix illumination of liquid crystal phase space light modulator in 3D imaging of APD array

NASA Astrophysics Data System (ADS)

Wang, Shuai; Sun, Huayan; Guo, Huichao

2018-01-01

Aiming at the problem of beam scanning in low-resolution APD array in three-dimensional imaging, a method of beam scanning with liquid crystal phase-space optical modulator is proposed to realize high-resolution imaging by low-resolution APD array. First, a liquid crystal phase spatial light modulator is used to generate a beam array and then a beam array is scanned. Since the sub-beam divergence angle in the beam array is smaller than the field angle of a single pixel in the APD array, the APD's pixels respond only to the three-dimensional information of the beam illumination position. Through the scanning of the beam array, a single pixel is used to collect the target three-dimensional information multiple times, thereby improving the resolution of the APD detector. Finally, MATLAB is used to simulate the algorithm in this paper by using two-dimensional scalar diffraction theory, which realizes the splitting and scanning with a resolution of 5 x 5. The feasibility is verified theoretically.

Experimental and theoretical analysis for improved microscope design of optical projection tomographic microscopy.

PubMed

Coe, Ryan L; Seibel, Eric J

2013-09-01

We present theoretical and experimental results of axial displacement of objects relative to a fixed condenser focal plane (FP) in optical projection tomographic microscopy (OPTM). OPTM produces three-dimensional, reconstructed images of single cells from two-dimensional projections. The cell rotates in a microcapillary to acquire projections from different perspectives where the objective FP is scanned through the cell while the condenser FP remains fixed at the center of the microcapillary. This work uses a combination of experimental and theoretical methods to improve the OPTM instrument design.

Three-dimensional Fourier-domain optical coherence tomography of alveolar mechanics in stepwise inflated and deflated isolated and perfused rabbit lungs

NASA Astrophysics Data System (ADS)

Krueger, Alexander; Knels, Lilla; Meissner, Sven; Wendel, Martina; Heller, Axel R.; Lambeck, Thomas; Koch, Thea; Koch, Edmund

2007-07-01

Fourier domain optical coherence tomography (FD-OCT) was used to acquire three-dimensional image stacks of isolated and perfused rabbit lungs (n = 4) at different constant pulmonary airway pressures (CPAP) and during vascular fixation. After despeckling and applying a threshold, the images were segmented into air and tissue, and registered to each other to compensate for movement between CPAP steps. The air-filled cross-sectional areas were quantified using a semi-automatic algorithm. The cross-sectional area of alveolar structures taken at all three perpendicular planes increased with increasing CPAP. Between the minimal CPAP of 3 mbar and the maximum of 25 mbar the areas increased to about 140% of their initial value. There was no systematic dependency of inflation rate on initial size of the alveolar structure. During the perfusion fixation of the lungs with glutaraldehyde morphometric changes of the alveolar geometry measured with FD-OCT were negligible.

Three-dimensional optical tomographic imaging of supersonic jets through inversion of phase data obtained through the transport-of-intensity equation.

PubMed

Hemanth, Thayyullathil; Rajesh, Langoju; Padmaram, Renganathan; Vasu, R Mohan; Rajan, Kanjirodan; Patnaik, Lalit M

2004-07-20

We report experimental results of quantitative imaging in supersonic circular jets by using a monochromatic light probe. An expanding cone of light interrogates a three-dimensional volume of a supersonic steady-state flow from a circular jet. The distortion caused to the spherical wave by the presence of the jet is determined through our measuring normal intensity transport. A cone-beam tomographic algorithm is used to invert wave-front distortion to changes in refractive index introduced by the flow. The refractive index is converted into density whose cross sections reveal shock and other characteristics of the flow.

Quantitative Fourier Domain Optical Coherence Tomography Imaging of the Ocular Anterior Segment

NASA Astrophysics Data System (ADS)

McNabb, Ryan Palmer

Clinical imaging within ophthalmology has had transformative effects on ocular health over the last century. Imaging has guided clinicians in their pharmaceutical and surgical treatments of macular degeneration, glaucoma, cataracts and numerous other pathologies. Many of the imaging techniques currently used are photography based and are limited to imaging the surface of ocular structures. This limitation forces clinicians to make assumptions about the underlying tissue which may reduce the efficacy of their diagnoses. Optical coherence tomography (OCT) is a non-invasive, non-ionizing imaging modality that has been widely adopted within the field of ophthalmology in the last 15 years. As an optical imaging technique, OCT utilizes low-coherence interferometry to produce micron-scale three-dimensional datasets of a tissue's structure. Much of the human body consists of tissues that significantly scatter and attenuate optical signals limiting the imaging depth of OCT in those tissues to only 1-2mm. However, the ocular anterior segment is unique among human tissue in that it is primarily transparent or translucent. This allows for relatively deep imaging of tissue structure with OCT and is no longer limited by the optical scattering properties of the tissue. This goal of this work is to develop methods utilizing OCT that offer the potential to reduce the assumptions made by clinicians in their evaluations of their patients' ocular anterior segments. We achieved this by first developing a method to reduce the effects of patient motion during OCT volume acquisitions allowing for accurate, three dimensional measurements of corneal shape. Having accurate corneal shape measurements then allowed us to determine corneal spherical and astigmatic refractive contribution in a given individual. This was then validated in a clinical study that showed OCT better measured refractive change due to surgery than other clinical devices. Additionally, a method was developed to combine the clinical evaluation of the iridocorneal angle through gonioscopy with OCT.

Microscopic Optical Projection Tomography In Vivo

PubMed Central

Meyer, Heiko; Ripoll, Jorge; Tavernarakis, Nektarios

2011-01-01

We describe a versatile optical projection tomography system for rapid three-dimensional imaging of microscopic specimens in vivo. Our tomographic setup eliminates the in xy and z strongly asymmetric resolution, resulting from optical sectioning in conventional confocal microscopy. It allows for robust, high resolution fluorescence as well as absorption imaging of live transparent invertebrate animals such as C. elegans. This system offers considerable advantages over currently available methods when imaging dynamic developmental processes and animal ageing; it permits monitoring of spatio-temporal gene expression and anatomical alterations with single-cell resolution, it utilizes both fluorescence and absorption as a source of contrast, and is easily adaptable for a range of small model organisms. PMID:21559481

Range Imaging without Moving Parts

NASA Technical Reports Server (NTRS)

Blair, J. Bryan; Scott, V. Stanley, III; Ramos-Izquierdo, Luis

2008-01-01

Range-imaging instruments of a type now under development are intended to generate the equivalent of three-dimensional images from measurements of the round-trip times of flight of laser pulses along known directions. These instruments could also provide information on characteristics of targets, including roughnesses and reflectivities of surfaces and optical densities of such semi-solid objects as trees and clouds. Unlike in prior range-imaging instruments based on times of flight along known directions, there would be no moving parts; aiming of the laser beams along the known directions would not be accomplished by mechanical scanning of mirrors, prisms, or other optical components. Instead, aiming would be accomplished by using solid-state devices to switch input and output beams along different fiber-optic paths. Because of the lack of moving parts, these instruments could be extraordinarily reliable, rugged, and long-lasting. An instrument of this type would include an optical transmitter that would send out a laser pulse along a chosen direction to a target. An optical receiver coaligned with the transmitter would measure the temporally varying intensity of laser light reflected from the target to determine the distance and surface characteristics of the target. The transmitter would be a combination of devices for generating precise directional laser illumination. It would include a pulsed laser, the output of which would be coupled into a fiber-optic cable with a fan-out and solid-state optical switches that would enable switching of the laser beam onto one or more optical fibers terminated at known locations in an array on a face at the focal plane of a telescope. The array would be imaged by the telescope onto the target space. The receiver optical system could share the aforementioned telescope with the transmitter or could include a separate telescope aimed in the same direction as that of the transmitting telescope. In either case, light reflected from the target would be focused by the receiver optical system onto an array of optical fibers matching the array in the transmitter. These optical fibers would couple the received light to one or more photodetector( s). Optionally, the receiver could include solid-state optical switches for choosing which optical fiber(s) would couple light to the photodetector(s). This instrument architecture is flexible and can be optimized for a wide variety of applications and levels of performance. For example, it is scalable to any number of pixels and pixel resolutions and is compatible with a variety of ranging and photodetection methodologies, including, for example, ranging by use of modulated (including pulsed and encoded) light signals. The use of fixed arrays of optical fibers to generate controlled illumination patterns would eliminate the mechanical complexity and much of the bulk of optomechanical scanning assemblies. Furthermore, digital control of the selection of the fiber-optic pathways for the transmitted beams could afford capabilities not seen in previous three-dimensional range-imaging systems. Instruments of this type could be specialized for use as, for example, proximity detectors, three-dimensional robotic vision systems, airborne terrain-mapping systems, and inspection systems.

Three-dimensional image and spatial spectrum analysis of behavior of small animal erythrocytes in optical tweezers

NASA Astrophysics Data System (ADS)

Chen, Hui Chi; Shen, Wen-Tai; Kong, Yu-Han; Chuang, Chun-Hao

2008-02-01

Because of the softness of membrane, erythrocytes (red blood cell, RBC) have different shapes while being immersed in buffer with different osmotic pressure. While affecting by different viruses and illnesses, RBC may change its shape, or its membrane may become rigid. Moreover, RBC will ford and stretch when it is trapped by optical tweezers. Therefore, the behaviors of RBC in optical tweezers raise more discussion. In this report, we set up an optical tweezers to trap RBC of small animals like feline and canine. By adding a long working distance objective to collect the side-viewing image, a 3-D image system was constructed to detect the motion of trapped RBC. To improve the image quality for side-view, an aperture and narrow glass plate were used. From the video of these images and their spatial spectrum, the shape of trapped RBC was studied.

Toward fully three-dimensional-printed miniaturized confocal imager

NASA Astrophysics Data System (ADS)

Savaş, Janset; Khayatzadeh, Ramin; Çivitçi, Fehmi; Gökdel, Yiğit Dağhan; Ferhanoğlu, Onur

2018-04-01

We present a disposable miniaturized confocal imager, consisting mostly of three-dimensional (3-D)-printed components. A 3-D printed laser scanner with 10×10 mm2 frame size is employed for Lissajous scan, with 180 and 315 Hz frequencies in orthogonal directions corresponding to ±8 deg and ±4 deg optical scan angles, respectively. The actuation is done electromagnetically via a magnet attached to the scanner and an external coil. A miniaturized lens with 6-mm clear aperture and 10-mm focal length is 3-D printed and postprocessed to obtain desired (≤λ/5 surface roughness) performance. All components are press-fitted into a 3-D-printed housing having 17 mm width, which is comparable to many of the MEMS-based scanning imagers. Finally, line-scan from a resolution target and two-dimensional scanning in the sample location were demonstrated with the integrated device.

Three-dimensional nanometre localization of nanoparticles to enhance super-resolution microscopy

NASA Astrophysics Data System (ADS)

Bon, Pierre; Bourg, Nicolas; Lécart, Sandrine; Monneret, Serge; Fort, Emmanuel; Wenger, Jérôme; Lévêque-Fort, Sandrine

2015-07-01

Meeting the nanometre resolution promised by super-resolution microscopy techniques (pointillist: PALM, STORM, scanning: STED) requires stabilizing the sample drifts in real time during the whole acquisition process. Metal nanoparticles are excellent probes to track the lateral drifts as they provide crisp and photostable information. However, achieving nanometre axial super-localization is still a major challenge, as diffraction imposes large depths-of-fields. Here we demonstrate fast full three-dimensional nanometre super-localization of gold nanoparticles through simultaneous intensity and phase imaging with a wavefront-sensing camera based on quadriwave lateral shearing interferometry. We show how to combine the intensity and phase information to provide the key to the third axial dimension. Presently, we demonstrate even in the occurrence of large three-dimensional fluctuations of several microns, unprecedented sub-nanometre localization accuracies down to 0.7 nm in lateral and 2.7 nm in axial directions at 50 frames per second. We demonstrate that nanoscale stabilization greatly enhances the image quality and resolution in direct stochastic optical reconstruction microscopy imaging.

Three-Dimensional Large Screen Display Using Polymer-Dispersed Liquid-Crystal Light Valves and a Schlieren Optical System: Proposal and Basic Experiments

NASA Astrophysics Data System (ADS)

Takizawa, Kuniharu

A novel three-dimensional (3-D) projection display used with polarized eyeglasses is proposed. It consists of polymer-dispersed liquid crystal-light valves that modulate the illuminated light based on light scattering, a polarization beam splitter, and a Schlieren projection system. The features of the proposed display include a 3-D image display with a single projector, half size and half power consumption compared with a conventional 3-D projector with polarized glasses. Measured electro-optic characteristics of a polymer-dispersed liquid-crystal cell inserted between crossed polarizers suggests that the proposed display achieves small cross talk and high-extinction ratio.

High-resolution non-destructive three-dimensional imaging of integrated circuits

NASA Astrophysics Data System (ADS)

Holler, Mirko; Guizar-Sicairos, Manuel; Tsai, Esther H. R.; Dinapoli, Roberto; Müller, Elisabeth; Bunk, Oliver; Raabe, Jörg; Aeppli, Gabriel

2017-03-01

Modern nanoelectronics has advanced to a point at which it is impossible to image entire devices and their interconnections non-destructively because of their small feature sizes and the complex three-dimensional structures resulting from their integration on a chip. This metrology gap implies a lack of direct feedback between design and manufacturing processes, and hampers quality control during production, shipment and use. Here we demonstrate that X-ray ptychography—a high-resolution coherent diffractive imaging technique—can create three-dimensional images of integrated circuits of known and unknown designs with a lateral resolution in all directions down to 14.6 nanometres. We obtained detailed device geometries and corresponding elemental maps, and show how the devices are integrated with each other to form the chip. Our experiments represent a major advance in chip inspection and reverse engineering over the traditional destructive electron microscopy and ion milling techniques. Foreseeable developments in X-ray sources, optics and detectors, as well as adoption of an instrument geometry optimized for planar rather than cylindrical samples, could lead to a thousand-fold increase in efficiency, with concomitant reductions in scan times and voxel sizes.

High-resolution non-destructive three-dimensional imaging of integrated circuits.

PubMed

Holler, Mirko; Guizar-Sicairos, Manuel; Tsai, Esther H R; Dinapoli, Roberto; Müller, Elisabeth; Bunk, Oliver; Raabe, Jörg; Aeppli, Gabriel

2017-03-15

Modern nanoelectronics has advanced to a point at which it is impossible to image entire devices and their interconnections non-destructively because of their small feature sizes and the complex three-dimensional structures resulting from their integration on a chip. This metrology gap implies a lack of direct feedback between design and manufacturing processes, and hampers quality control during production, shipment and use. Here we demonstrate that X-ray ptychography-a high-resolution coherent diffractive imaging technique-can create three-dimensional images of integrated circuits of known and unknown designs with a lateral resolution in all directions down to 14.6 nanometres. We obtained detailed device geometries and corresponding elemental maps, and show how the devices are integrated with each other to form the chip. Our experiments represent a major advance in chip inspection and reverse engineering over the traditional destructive electron microscopy and ion milling techniques. Foreseeable developments in X-ray sources, optics and detectors, as well as adoption of an instrument geometry optimized for planar rather than cylindrical samples, could lead to a thousand-fold increase in efficiency, with concomitant reductions in scan times and voxel sizes.

Three-dimensional reconstruction of highly complex microscopic samples using scanning electron microscopy and optical flow estimation.

PubMed

Baghaie, Ahmadreza; Pahlavan Tafti, Ahmad; Owen, Heather A; D'Souza, Roshan M; Yu, Zeyun

2017-01-01

Scanning Electron Microscope (SEM) as one of the major research and industrial equipment for imaging of micro-scale samples and surfaces has gained extensive attention from its emerge. However, the acquired micrographs still remain two-dimensional (2D). In the current work a novel and highly accurate approach is proposed to recover the hidden third-dimension by use of multi-view image acquisition of the microscopic samples combined with pre/post-processing steps including sparse feature-based stereo rectification, nonlocal-based optical flow estimation for dense matching and finally depth estimation. Employing the proposed approach, three-dimensional (3D) reconstructions of highly complex microscopic samples were achieved to facilitate the interpretation of topology and geometry of surface/shape attributes of the samples. As a byproduct of the proposed approach, high-definition 3D printed models of the samples can be generated as a tangible means of physical understanding. Extensive comparisons with the state-of-the-art reveal the strength and superiority of the proposed method in uncovering the details of the highly complex microscopic samples.

Resolution enhancement using simultaneous couple illumination

NASA Astrophysics Data System (ADS)

Hussain, Anwar; Martínez Fuentes, José Luis

2016-10-01

A super-resolution technique based on structured illumination created by a liquid crystal on silicon spatial light modulator (LCOS-SLM) is presented. Single and simultaneous pairs of tilted beams are generated to illuminate a target object. Resolution enhancement of an optical 4f system is demonstrated by using numerical simulations. The resulting intensity images are recorded at a charged couple device (CCD) and stored in the computer memory for further processing. One dimension enhancement can be performed with only 15 images. Two dimensional complete improvement requires 153 different images. The resolution of the optical system is extended three times compared to the band limited system.

Truly simultaneous SS-OCT of the anterior and posterior human eye with full anterior chamber and 50° retinal field of views (Conference Presentation)

NASA Astrophysics Data System (ADS)

McNabb, Ryan P.; Viehland, Christian; Keller, Brenton; Vann, Robin R.; Izatt, Joseph A.; Kuo, Anthony N.

2017-02-01

Optical coherence tomography (OCT) has revolutionized clinical observation of the eye and is an indispensable part of the modern ophthalmic practice. Unlike many other ophthalmic imaging techniques, OCT provides three-dimensional information about the imaged eye. However, conventional clinical OCT systems image only the anterior or the posterior eye during a single acquisition. Newer OCT systems have begun to image both during the same acquisition but with compromises such as limited field of view in the posterior eye or requiring rapid switching between the anterior and posterior eye during the scan. We describe here the development and demonstration of an OCT system with truly simultaneous imaging of both the anterior and posterior eye capable of imaging the full anterior chamber width and 50° on the retina (macula, optic nerve, and arcades). The whole eye OCT system was developed using custom optics and optomechanics. Polarization was utilized to separate the imaging channels. We utilized a 200kHz swept-source laser (Axsun Technologies) centered at 1040±50nm of bandwidth. The clock signal generated by the laser was interpolated 4x to generate 5504 samples per laser sweep. With the whole eye OCT system, we simultaneously acquired anterior and posterior segments with repeated B-scans as well as three-dimensional volumes from seven healthy volunteers (other than refractive error). On three of these volunteers, whole eye OCT and partial coherence interferometry (LenStar PCI, Haag-Streit) were used to measure axial eye length. We measured a mean repeatability of ±47µm with whole eye OCT and a mean difference from PCI of -68µm.

Three-dimension imaging lidar

NASA Technical Reports Server (NTRS)

Degnan, John J. (Inventor)

2007-01-01

This invention is directed to a 3-dimensional imaging lidar, which utilizes modest power kHz rate lasers, array detectors, photon-counting multi-channel timing receivers, and dual wedge optical scanners with transmitter point-ahead correction to provide contiguous high spatial resolution mapping of surface features including ground, water, man-made objects, vegetation and submerged surfaces from an aircraft or a spacecraft.

Development of x-ray laminography under an x-ray microscopic condition

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hoshino, Masato; Uesugi, Kentaro; Takeuchi, Akihisa

2011-07-15

An x-ray laminography system under an x-ray microscopic condition was developed to obtain a three-dimensional structure of laterally-extended planar objects which were difficult to observe by x-ray tomography. An x-ray laminography technique was introduced to an x-ray transmission microscope with zone plate optics. Three prototype sample holders were evaluated for x-ray imaging laminography. Layered copper grid sheets were imaged as a laminated sample. Diatomite powder on a silicon nitride membrane was measured to confirm the applicability of this method to non-planar micro-specimens placed on the membrane. The three-dimensional information of diatom shells on the membrane was obtained at a spatialmore » resolution of sub-micron. Images of biological cells on the membrane were also obtained by using a Zernike phase contrast technique.« less

Synchromodal optical in vivo imaging employing microlens array optics: a complete framework

NASA Astrophysics Data System (ADS)

Peter, Joerg

2013-03-01

A complete mathematical framework for preclinical optical imaging (OI) support comprising bioluminescence imaging (BLI), fluorescence surface imaging (FSI) and fluorescence optical tomography (FOT) is presented in which optical data is acquired by means of a microlens array (MLA) based light detector (MLA-D). The MLA-D has been developed to enable unique OI, especially in synchromodal operation with secondary imaging modalities (SIM) such as positron emission tomography (PET) or magnetic resonance imaging (MRI). An MLA-D consists of a (large-area) photon sensor array, a matched MLA for field-of-view definition, and a septum mask of specific geometry made of anodized aluminum that is positioned between the sensor and the MLA to suppresses light cross-talk and to shield the sensor's radiofrequency interference signal (essential when used inside an MRI system). The software framework, while freely parameterizable for any MLA-D, is tailored towards an OI prototype system for preclinical SIM application comprising a multitude of cylindrically assembled, gantry-mounted, simultaneously operating MLA-D's. Besides the MLA-D specificity, the framework incorporates excitation and illumination light-source declarations of large-field and point geometry to facilitate multispectral FSI and FOT as well as three-dimensional object recognition. When used in synchromodal operation, reconstructed tomographic SIM volume data can be used for co-modal image fusion and also as a prior for estimating the imaged object's 3D surface by means of gradient vector flow. Superimposed planar (without object prior) or surface-aligned inverse mapping can be performed to estimate and to fuse the emission light map with the boundary of the imaged object. Triangulation and subsequent optical reconstruction (FOT) or constrained flow estimation (BLI), both including the possibility of SIM priors, can be performed to estimate the internal three-dimensional emission light distribution. The framework is susceptible to a number of variables controlling convergence and computational speed. Utilization and performance is illustrated on experimentally acquired data employing the OI prototype system in stand-alone operation, and when integrated into an unmodified preclinical PET system performing synchromodal BLI-PET in vivo imaging.

Double-clad photonic crystal fiber coupler for compact nonlinear optical microscopy imaging.

PubMed

Fu, Ling; Gu, Min

2006-05-15

A 1 x 2 double-clad photonic crystal fiber coupler is fabricated by the fused tapered method, showing a low excess loss of 1.1 dB and a splitting ratio of 97/3 over the entire visible and near-infrared wavelength range. In addition to the property of splitting the laser power, the double-clad feature of the coupler facilitates the separation of a near-infrared single-mode beam from a visible multimode beam, which is ideal for nonlinear optical microscopy imaging. In conjunction with a gradient-index lens, this coupler is used to construct a miniaturized microscope based on two-photon fluorescence and second-harmonic generation. Three-dimensional nonlinear optical images demonstrate potential applications of the coupler to compact all-fiber and nonlinear optical microscopy and endoscopy.

Three-dimensional measurement of small inner surface profiles using feature-based 3-D panoramic registration

PubMed Central

Gong, Yuanzheng; Seibel, Eric J.

2017-01-01

Rapid development in the performance of sophisticated optical components, digital image sensors, and computer abilities along with decreasing costs has enabled three-dimensional (3-D) optical measurement to replace more traditional methods in manufacturing and quality control. The advantages of 3-D optical measurement, such as noncontact, high accuracy, rapid operation, and the ability for automation, are extremely valuable for inline manufacturing. However, most of the current optical approaches are eligible for exterior instead of internal surfaces of machined parts. A 3-D optical measurement approach is proposed based on machine vision for the 3-D profile measurement of tiny complex internal surfaces, such as internally threaded holes. To capture the full topographic extent (peak to valley) of threads, a side-view commercial rigid scope is used to collect images at known camera positions and orientations. A 3-D point cloud is generated with multiview stereo vision using linear motion of the test piece, which is repeated by a rotation to form additional point clouds. Registration of these point clouds into a complete reconstruction uses a proposed automated feature-based 3-D registration algorithm. The resulting 3-D reconstruction is compared with x-ray computed tomography to validate the feasibility of our proposed method for future robotically driven industrial 3-D inspection. PMID:28286351

Three-dimensional measurement of small inner surface profiles using feature-based 3-D panoramic registration

NASA Astrophysics Data System (ADS)

Gong, Yuanzheng; Seibel, Eric J.

2017-01-01

Rapid development in the performance of sophisticated optical components, digital image sensors, and computer abilities along with decreasing costs has enabled three-dimensional (3-D) optical measurement to replace more traditional methods in manufacturing and quality control. The advantages of 3-D optical measurement, such as noncontact, high accuracy, rapid operation, and the ability for automation, are extremely valuable for inline manufacturing. However, most of the current optical approaches are eligible for exterior instead of internal surfaces of machined parts. A 3-D optical measurement approach is proposed based on machine vision for the 3-D profile measurement of tiny complex internal surfaces, such as internally threaded holes. To capture the full topographic extent (peak to valley) of threads, a side-view commercial rigid scope is used to collect images at known camera positions and orientations. A 3-D point cloud is generated with multiview stereo vision using linear motion of the test piece, which is repeated by a rotation to form additional point clouds. Registration of these point clouds into a complete reconstruction uses a proposed automated feature-based 3-D registration algorithm. The resulting 3-D reconstruction is compared with x-ray computed tomography to validate the feasibility of our proposed method for future robotically driven industrial 3-D inspection.

Three-dimensional labeling program for elucidation of the geometric properties of biological particles in three-dimensional space.

PubMed

Nomura, A; Yamazaki, Y; Tsuji, T; Kawasaki, Y; Tanaka, S

1996-09-15

For all biological particles such as cells or cellular organelles, there are three-dimensional coordinates representing the centroid or center of gravity. These coordinates and other numerical parameters such as volume, fluorescence intensity, surface area, and shape are referred to in this paper as geometric properties, which may provide critical information for the clarification of in situ mechanisms of molecular and cellular functions in living organisms. We have established a method for the elucidation of these properties, designated the three-dimensional labeling program (3DLP). Algorithms of 3DLP are so simple that this method can be carried out through the use of software combinations in image analysis on a personal computer. To evaluate 3DLP, it was applied to a 32-cell-stage sea urchin embryo, double stained with FITC for cellular protein of blastomeres and propidium iodide for nuclear DNA. A stack of optical serial section images was obtained by confocal laser scanning microscopy. The method was found effective for determining geometric properties and should prove applicable to the study of many different kinds of biological particles in three-dimensional space.

Memory-effect based deconvolution microscopy for super-resolution imaging through scattering media

NASA Astrophysics Data System (ADS)

Edrei, Eitan; Scarcelli, Giuliano

2016-09-01

High-resolution imaging through turbid media is a fundamental challenge of optical sciences that has attracted a lot of attention in recent years for its wide range of potential applications. Here, we demonstrate that the resolution of imaging systems looking behind a highly scattering medium can be improved below the diffraction-limit. To achieve this, we demonstrate a novel microscopy technique enabled by the optical memory effect that uses a deconvolution image processing and thus it does not require iterative focusing, scanning or phase retrieval procedures. We show that this newly established ability of direct imaging through turbid media provides fundamental and practical advantages such as three-dimensional refocusing and unambiguous object reconstruction.

Memory-effect based deconvolution microscopy for super-resolution imaging through scattering media.

PubMed

Edrei, Eitan; Scarcelli, Giuliano

2016-09-16

High-resolution imaging through turbid media is a fundamental challenge of optical sciences that has attracted a lot of attention in recent years for its wide range of potential applications. Here, we demonstrate that the resolution of imaging systems looking behind a highly scattering medium can be improved below the diffraction-limit. To achieve this, we demonstrate a novel microscopy technique enabled by the optical memory effect that uses a deconvolution image processing and thus it does not require iterative focusing, scanning or phase retrieval procedures. We show that this newly established ability of direct imaging through turbid media provides fundamental and practical advantages such as three-dimensional refocusing and unambiguous object reconstruction.

Application of Least-Squares Adjustment Technique to Geometric Camera Calibration and Photogrammetric Flow Visualization

NASA Technical Reports Server (NTRS)

Chen, Fang-Jenq

1997-01-01

Flow visualization produces data in the form of two-dimensional images. If the optical components of a camera system are perfect, the transformation equations between the two-dimensional image and the three-dimensional object space are linear and easy to solve. However, real camera lenses introduce nonlinear distortions that affect the accuracy of transformation unless proper corrections are applied. An iterative least-squares adjustment algorithm is developed to solve the nonlinear transformation equations incorporated with distortion corrections. Experimental applications demonstrate that a relative precision on the order of 40,000 is achievable without tedious laboratory calibrations of the camera.

Wavelet compression techniques for hyperspectral data

NASA Technical Reports Server (NTRS)

Evans, Bruce; Ringer, Brian; Yeates, Mathew

1994-01-01

Hyperspectral sensors are electro-optic sensors which typically operate in visible and near infrared bands. Their characteristic property is the ability to resolve a relatively large number (i.e., tens to hundreds) of contiguous spectral bands to produce a detailed profile of the electromagnetic spectrum. In contrast, multispectral sensors measure relatively few non-contiguous spectral bands. Like multispectral sensors, hyperspectral sensors are often also imaging sensors, measuring spectra over an array of spatial resolution cells. The data produced may thus be viewed as a three dimensional array of samples in which two dimensions correspond to spatial position and the third to wavelength. Because they multiply the already large storage/transmission bandwidth requirements of conventional digital images, hyperspectral sensors generate formidable torrents of data. Their fine spectral resolution typically results in high redundancy in the spectral dimension, so that hyperspectral data sets are excellent candidates for compression. Although there have been a number of studies of compression algorithms for multispectral data, we are not aware of any published results for hyperspectral data. Three algorithms for hyperspectral data compression are compared. They were selected as representatives of three major approaches for extending conventional lossy image compression techniques to hyperspectral data. The simplest approach treats the data as an ensemble of images and compresses each image independently, ignoring the correlation between spectral bands. The second approach transforms the data to decorrelate the spectral bands, and then compresses the transformed data as a set of independent images. The third approach directly generalizes two-dimensional transform coding by applying a three-dimensional transform as part of the usual transform-quantize-entropy code procedure. The algorithms studied all use the discrete wavelet transform. In the first two cases, a wavelet transform coder was used for the two-dimensional compression. The third case used a three dimensional extension of this same algorithm.

Correction of motion artifacts in endoscopic optical coherence tomography and autofluorescence images based on azimuthal en face image registration.

PubMed

Abouei, Elham; Lee, Anthony M D; Pahlevaninezhad, Hamid; Hohert, Geoffrey; Cua, Michelle; Lane, Pierre; Lam, Stephen; MacAulay, Calum

2018-01-01

We present a method for the correction of motion artifacts present in two- and three-dimensional in vivo endoscopic images produced by rotary-pullback catheters. This method can correct for cardiac/breathing-based motion artifacts and catheter-based motion artifacts such as nonuniform rotational distortion (NURD). This method assumes that en face tissue imaging contains slowly varying structures that are roughly parallel to the pullback axis. The method reduces motion artifacts using a dynamic time warping solution through a cost matrix that measures similarities between adjacent frames in en face images. We optimize and demonstrate the suitability of this method using a real and simulated NURD phantom and in vivo endoscopic pulmonary optical coherence tomography and autofluorescence images. Qualitative and quantitative evaluations of the method show an enhancement of the image quality. (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

Hindered diffusion of high molecular weight compounds in brain extracellular microenvironment measured with integrative optical imaging.

PubMed

Nicholson, C; Tao, L

1993-12-01

This paper describes the theory of an integrative optical imaging system and its application to the analysis of the diffusion of 3-, 10-, 40-, and 70-kDa fluorescent dextran molecules in agarose gel and brain extracellular microenvironment. The method uses a precisely defined source of fluorescent molecules pressure ejected from a micropipette, and a detailed theory of the intensity contributions from out-of-focus molecules in a three-dimensional medium to a two-dimensional image. Dextrans tagged with either tetramethylrhodamine or Texas Red were ejected into 0.3% agarose gel or rat cortical slices maintained in a perfused chamber at 34 degrees C and imaged using a compound epifluorescent microscope with a 10 x water-immersion objective. About 20 images were taken at 2-10-s intervals, recorded with a cooled CCD camera, then transferred to a 486 PC for quantitative analysis. The diffusion coefficient in agarose gel, D, and the apparent diffusion coefficient, D*, in brain tissue were determined by fitting an integral expression relating the measured two-dimensional image intensity to the theoretical three-dimensional dextran concentration. The measurements in dilute agarose gel provided a reference value of D and validated the method. Values of the tortuosity, lambda = (D/D*)1/2, for the 3- and 10-kDa dextrans were 1.70 and 1.63, respectively, which were consistent with previous values derived from tetramethylammonium measurements in cortex. Tortuosities for the 40- and 70-kDa dextrans had significantly larger values of 2.16 and 2.25, respectively. This suggests that the extracellular space may have local constrictions that hinder the diffusion of molecules above a critical size that lies in the range of many neurotrophic compounds.

Functional imaging of the human brain using a modular, fibre-less, high-density diffuse optical tomography system.

PubMed

Chitnis, Danial; Cooper, Robert J; Dempsey, Laura; Powell, Samuel; Quaggia, Simone; Highton, David; Elwell, Clare; Hebden, Jeremy C; Everdell, Nicholas L

2016-10-01

We present the first three-dimensional, functional images of the human brain to be obtained using a fibre-less, high-density diffuse optical tomography system. Our technology consists of independent, miniaturized, silicone-encapsulated DOT modules that can be placed directly on the scalp. Four of these modules were arranged to provide up to 128, dual-wavelength measurement channels over a scalp area of approximately 60 × 65 mm 2 . Using a series of motor-cortex stimulation experiments, we demonstrate that this system can obtain high-quality, continuous-wave measurements at source-detector separations ranging from 14 to 55 mm in adults, in the presence of hair. We identify robust haemodynamic response functions in 5 out of 5 subjects, and present diffuse optical tomography images that depict functional haemodynamic responses that are well-localized in all three dimensions at both the individual and group levels. This prototype modular system paves the way for a new generation of wearable, wireless, high-density optical neuroimaging technologies.

Optimal Filter Estimation for Lucas-Kanade Optical Flow

PubMed Central

Sharmin, Nusrat; Brad, Remus

2012-01-01

Optical flow algorithms offer a way to estimate motion from a sequence of images. The computation of optical flow plays a key-role in several computer vision applications, including motion detection and segmentation, frame interpolation, three-dimensional scene reconstruction, robot navigation and video compression. In the case of gradient based optical flow implementation, the pre-filtering step plays a vital role, not only for accurate computation of optical flow, but also for the improvement of performance. Generally, in optical flow computation, filtering is used at the initial level on original input images and afterwards, the images are resized. In this paper, we propose an image filtering approach as a pre-processing step for the Lucas-Kanade pyramidal optical flow algorithm. Based on a study of different types of filtering methods and applied on the Iterative Refined Lucas-Kanade, we have concluded on the best filtering practice. As the Gaussian smoothing filter was selected, an empirical approach for the Gaussian variance estimation was introduced. Tested on the Middlebury image sequences, a correlation between the image intensity value and the standard deviation value of the Gaussian function was established. Finally, we have found that our selection method offers a better performance for the Lucas-Kanade optical flow algorithm.

Combined magnetic resonance, fluorescence, and histology imaging strategy in a human breast tumor xenograft model

PubMed Central

Jiang, Lu; Greenwood, Tiffany R.; Amstalden van Hove, Erika R.; Chughtai, Kamila; Raman, Venu; Winnard, Paul T.; Heeren, Ron; Artemov, Dmitri; Glunde, Kristine

2014-01-01

Applications of molecular imaging in cancer and other diseases frequently require combining in vivo imaging modalities, such as magnetic resonance and optical imaging, with ex vivo optical, fluorescence, histology, and immunohistochemical (IHC) imaging, to investigate and relate molecular and biological processes to imaging parameters within the same region of interest. We have developed a multimodal image reconstruction and fusion framework that accurately combines in vivo magnetic resonance imaging (MRI) and magnetic resonance spectroscopic imaging (MRSI), ex vivo brightfield and fluorescence microscopic imaging, and ex vivo histology imaging. Ex vivo brightfield microscopic imaging was used as an intermediate modality to facilitate the ultimate link between ex vivo histology and in vivo MRI/MRSI. Tissue sectioning necessary for optical and histology imaging required generation of a three-dimensional (3D) reconstruction module for 2D ex vivo optical and histology imaging data. We developed an external fiducial marker based 3D reconstruction method, which was able to fuse optical brightfield and fluorescence with histology imaging data. Registration of 3D tumor shape was pursued to combine in vivo MRI/MRSI and ex vivo optical brightfield and fluorescence imaging data. This registration strategy was applied to in vivo MRI/MRSI, ex vivo optical brightfield/fluorescence, as well as histology imaging data sets obtained from human breast tumor models. 3D human breast tumor data sets were successfully reconstructed and fused with this platform. PMID:22945331

Optical encryption interface

NASA Technical Reports Server (NTRS)

Jackson, Deborah J. (Inventor)

1998-01-01

An analog optical encryption system based on phase scrambling of two-dimensional optical images and holographic transformation for achieving large encryption keys and high encryption speed. An enciphering interface uses a spatial light modulator for converting a digital data stream into a two dimensional optical image. The optical image is further transformed into a hologram with a random phase distribution. The hologram is converted into digital form for transmission over a shared information channel. A respective deciphering interface at a receiver reverses the encrypting process by using a phase conjugate reconstruction of the phase scrambled hologram.

Notes on Experiments.

ERIC Educational Resources Information Center

Physics Education, 1989

1989-01-01

Described are the purposes, laboratory set-ups, and procedures of four classroom experiments: ultrasound speedometer; vibrating-bar depth gauge; folding three-dimensional model of equipotential surfaces; and a simple optical system for the reconstruction of images from computer-generated holograms. Diagrams and pictures are provided. (YP)

Improving your four-dimensional image: traveling through a decade of light-sheet-based fluorescence microscopy research.

PubMed

Strobl, Frederic; Schmitz, Alexander; Stelzer, Ernst H K

2017-06-01

Light-sheet-based fluorescence microscopy features optical sectioning in the excitation process. This reduces phototoxicity and photobleaching by up to four orders of magnitude compared with that caused by confocal fluorescence microscopy, simplifies segmentation and quantification for three-dimensional cell biology, and supports the transition from on-demand to systematic data acquisition in developmental biology applications.

Building Facade Reconstruction by Fusing Terrestrial Laser Points and Images

PubMed Central

Pu, Shi; Vosselman, George

2009-01-01

Laser data and optical data have a complementary nature for three dimensional feature extraction. Efficient integration of the two data sources will lead to a more reliable and automated extraction of three dimensional features. This paper presents a semiautomatic building facade reconstruction approach, which efficiently combines information from terrestrial laser point clouds and close range images. A building facade's general structure is discovered and established using the planar features from laser data. Then strong lines in images are extracted using Canny extractor and Hough transformation, and compared with current model edges for necessary improvement. Finally, textures with optimal visibility are selected and applied according to accurate image orientations. Solutions to several challenge problems throughout the collaborated reconstruction, such as referencing between laser points and multiple images and automated texturing, are described. The limitations and remaining works of this approach are also discussed. PMID:22408539

Multiparametric optical coherence tomography imaging of the inner retinal hemodynamic response to visual stimulation

NASA Astrophysics Data System (ADS)

Radhakrishnan, Harsha; Srinivasan, Vivek J.

2013-08-01

The hemodynamic response to neuronal activation is a well-studied phenomenon in the brain, due to the prevalence of functional magnetic resonance imaging. The retina represents an optically accessible platform for studying lamina-specific neurovascular coupling in the central nervous system; however, due to methodological limitations, this has been challenging to date. We demonstrate techniques for the imaging of visual stimulus-evoked hyperemia in the rat inner retina using Doppler optical coherence tomography (OCT) and OCT angiography. Volumetric imaging with three-dimensional motion correction, en face flow calculation, and normalization of dynamic signal to static signal are techniques that reduce spurious changes caused by motion. We anticipate that OCT imaging of retinal functional hyperemia may yield viable biomarkers in diseases, such as diabetic retinopathy, where the neurovascular unit may be impaired.

Optical asymmetric image encryption using gyrator wavelet transform

NASA Astrophysics Data System (ADS)

Mehra, Isha; Nishchal, Naveen K.

2015-11-01

In this paper, we propose a new optical information processing tool termed as gyrator wavelet transform to secure a fully phase image, based on amplitude- and phase-truncation approach. The gyrator wavelet transform constitutes four basic parameters; gyrator transform order, type and level of mother wavelet, and position of different frequency bands. These parameters are used as encryption keys in addition to the random phase codes to the optical cryptosystem. This tool has also been applied for simultaneous compression and encryption of an image. The system's performance and its sensitivity to the encryption parameters, such as, gyrator transform order, and robustness has also been analyzed. It is expected that this tool will not only update current optical security systems, but may also shed some light on future developments. The computer simulation results demonstrate the abilities of the gyrator wavelet transform as an effective tool, which can be used in various optical information processing applications, including image encryption, and image compression. Also this tool can be applied for securing the color image, multispectral, and three-dimensional images.

Passive lighting responsive three-dimensional integral imaging

NASA Astrophysics Data System (ADS)

Lou, Yimin; Hu, Juanmei

2017-11-01

A three dimensional (3D) integral imaging (II) technique with a real-time passive lighting responsive ability and vivid 3D performance has been proposed and demonstrated. Some novel lighting responsive phenomena, including light-activated 3D imaging, and light-controlled 3D image scaling and translation, have been realized optically without updating images. By switching the on/off state of a point light source illuminated on the proposed II system, the 3D images can show/hide independent of the diffused illumination background. By changing the position or illumination direction of the point light source, the position and magnification of the 3D image can be modulated in real time. The lighting responsive mechanism of the 3D II system is deduced analytically and verified experimentally. A flexible thin film lighting responsive II system with a 0.4 mm thickness was fabricated. This technique gives some additional degrees of freedom in order to design the II system and enable the virtual 3D image to interact with the real illumination environment in real time.

Three-dimensional hard and soft tissue imaging of the human cochlea by scanning laser optical tomography (SLOT)

PubMed Central

Mohebbi, Saleh; Andrade, José; Nolte, Lena; Meyer, Heiko; Heisterkamp, Alexander; Majdani, Omid

2017-01-01

The present study focuses on the application of scanning laser optical tomography (SLOT) for visualization of anatomical structures inside the human cochlea ex vivo. SLOT is a laser-based highly efficient microscopy technique which allows for tomographic imaging of the internal structure of transparent specimens. Thus, in the field of otology this technique is best convenient for an ex vivo study of the inner ear anatomy. For this purpose, the preparation before imaging comprises decalcification, dehydration as well as optical clearing of the cochlea samples in toto. Here, we demonstrate results of SLOT imaging visualizing hard and soft tissue structures with an optical resolution of down to 15 μm using extinction and autofluorescence as contrast mechanisms. Furthermore, the internal structure can be analyzed nondestructively and quantitatively in detail by sectioning of the three-dimensional datasets. The method of X-ray Micro Computed Tomography (μCT) has been previously applied to explanted cochlea and is solely based on absorption contrast. An advantage of SLOT is that it uses visible light for image formation and thus provides a variety of contrast mechanisms known from other light microscopy techniques, such as fluorescence or scattering. We show that SLOT data is consistent with μCT anatomical data and provides additional information by using fluorescence. We demonstrate that SLOT is applicable for cochlea with metallic cochlear implants (CI) that would lead to significant artifacts in μCT imaging. In conclusion, the present study demonstrates the capability of SLOT for resolution visualization of cleared human cochleae ex vivo using multiple contrast mechanisms and lays the foundation for a broad variety of additional studies. PMID:28873437

Experimental research of digital holographic microscopic measuring

NASA Astrophysics Data System (ADS)

Zhu, Xueliang; Chen, Feifei; Li, Jicheng

2013-06-01

Digital holography is a new imaging technique, which is developed on the base of optical holography, Digital processing, and Computer techniques. It is using CCD instead of the conventional silver to record hologram, and then reproducing the 3D contour of the object by the way of computer simulation. Compared with the traditional optical holographic, the whole process is of simple measuring, lower production cost, faster the imaging speed, and with the advantages of non-contact real-time measurement. At present, it can be used in the fields of the morphology detection of tiny objects, micro deformation analysis, and biological cells shape measurement. It is one of the research hot spot at home and abroad. This paper introduced the basic principles and relevant theories about the optical holography and Digital holography, and researched the basic questions which influence the reproduce images in the process of recording and reconstructing of the digital holographic microcopy. In order to get a clear digital hologram, by analyzing the optical system structure, we discussed the recording distance and of the hologram. On the base of the theoretical studies, we established a measurement and analyzed the experimental conditions, then adjusted them to the system. To achieve a precise measurement of tiny object in three-dimension, we measured MEMS micro device for example, and obtained the reproduction three-dimensional contour, realized the three dimensional profile measurement of tiny object. According to the experiment results consider: analysis the reference factors between the zero-order term and a pair of twin-images by the choice of the object light and the reference light and the distance of the recording and reconstructing and the characteristics of reconstruction light on the measurement, the measurement errors were analyzed. The research result shows that the device owns certain reliability.

An overview of methods to mitigate artifacts in optical coherence tomography imaging of the skin.

PubMed

Adabi, Saba; Fotouhi, Audrey; Xu, Qiuyun; Daveluy, Steve; Mehregan, Darius; Podoleanu, Adrian; Nasiriavanaki, Mohammadreza

2018-05-01

Optical coherence tomography (OCT) of skin delivers three-dimensional images of tissue microstructures. Although OCT imaging offers a promising high-resolution modality, OCT images suffer from some artifacts that lead to misinterpretation of tissue structures. Therefore, an overview of methods to mitigate artifacts in OCT imaging of the skin is of paramount importance. Speckle, intensity decay, and blurring are three major artifacts in OCT images. Speckle is due to the low coherent light source used in the configuration of OCT. Intensity decay is a deterioration of light with respect to depth, and blurring is the consequence of deficiencies of optical components. Two speckle reduction methods (one based on artificial neural network and one based on spatial compounding), an attenuation compensation algorithm (based on Beer-Lambert law) and a deblurring procedure (using deconvolution), are described. Moreover, optical properties extraction algorithm based on extended Huygens-Fresnel (EHF) principle to obtain some additional information from OCT images are discussed. In this short overview, we summarize some of the image enhancement algorithms for OCT images which address the abovementioned artifacts. The results showed a significant improvement in the visibility of the clinically relevant features in the images. The quality improvement was evaluated using several numerical assessment measures. Clinical dermatologists benefit from using these image enhancement algorithms to improve OCT diagnosis and essentially function as a noninvasive optical biopsy. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Visualization of hair follicles using high-speed optical coherence tomography based on a Fourier domain mode locking laser

NASA Astrophysics Data System (ADS)

Tsai, M.-T.; Chang, F.-Y.

2012-04-01

In this study, a swept-source optical coherence tomography (SS-OCT) system with a Fourier domain mode locking (FDML) laser is proposed for a dermatology study. The homemade FDML laser is one kind of frequency-sweeping light source, which can provide output power of >20 mW and an output spectrum of 65 nm in bandwidth centered at 1300 nm, enabling imaging with an axial resolution of 12 μm in the OCT system. To eliminate the forward scans from the laser output and insert the delayed backward scans, a Mach-Zehnder configuration is implemented. Compared with conventional frequency-sweeping light sources, the FDML laser can achieve much higher scan rates, as high as ˜240 kHz, which can provide a three-dimensional imaging rate of 4 volumes/s. Furthermore, the proposed high-speed SS-OCT system can provide three-dimensional (3D) images with reduced motion artifacts. Finally, a high-speed SS-OCT system is used to visualize hair follicles, demonstrating the potential of this technology as a tool for noninvasive diagnosis of alopecia.

Three-dimensional calibration targets for optical coherence tomography

NASA Astrophysics Data System (ADS)

Gabriele Sandrian, Michelle; Tomlins, Pete; Woolliams, Peter; Rasakanthan, Janarthanan; Lee, Graham C.; Yang, Anna; Považay, Boris; Alex, Aneesh; Sugden, Kate; Drexler, Wolfgang

2012-03-01

The recent expansion of clinical applications for optical coherence tomography (OCT) is driving the development of approaches for consistent image acquisition. There is a simultaneous need for time-stable, easy-to-use imaging targets for calibration and standardization of OCT devices. We present calibration targets consisting of three-dimensional structures etched into nanoparticle-embedded resin. Spherical iron oxide nanoparticles with a predominant particle diameter of 400 nm were homogeneously dispersed in a two part polyurethane resin and allowed to harden overnight. These samples were then etched using a precision micromachining femtosecond laser with a center wavelength of 1026 nm, 100kHz repetition rate and 450 fs pulse duration. A series of lines in depth were etched, varying the percentage of inscription energy and speed of the translation stage moving the target with respect to the laser. Samples were imaged with a dual wavelength spectral-domain OCT system (λ=800nm, ▵λ~180nm, and λ=1325nm, ▵λ~100nm) and point-spread function of nanoparticles within the target was measured.

Reconstruction of Mammary Gland Structure Using Three-Dimensional Computer-Based Microscopy

DTIC Science & Technology

2004-08-01

for image analysis in cytology" Ortiz de Solorzano C., R . Malladi , Lockett S. In: Geometric methods in bio-medical image processing. Ravikanth Malladi ...Deschamps T., Idica A.K., Malladi R ., Ortiz de Solorzano C. Journal of Biomedical Optics 9(3):445-453, 2004.. Manuscripts (in preparation): "* "Three...Deschamps T., Idica A.K., 16 Malladi R ., Ortiz de Solorzano C., Proceedings of Photonics West 2003, Vol. 4964, 2003 "* "Automatic and segmentation

Digital and optical shape representation and pattern recognition; Proceedings of the Meeting, Orlando, FL, Apr. 4-6, 1988

NASA Technical Reports Server (NTRS)

Juday, Richard D. (Editor)

1988-01-01

The present conference discusses topics in pattern-recognition correlator architectures, digital stereo systems, geometric image transformations and their applications, topics in pattern recognition, filter algorithms, object detection and classification, shape representation techniques, and model-based object recognition methods. Attention is given to edge-enhancement preprocessing using liquid crystal TVs, massively-parallel optical data base management, three-dimensional sensing with polar exponential sensor arrays, the optical processing of imaging spectrometer data, hybrid associative memories and metric data models, the representation of shape primitives in neural networks, and the Monte Carlo estimation of moment invariants for pattern recognition.

Large depth of focus dynamic micro integral imaging for optical see-through augmented reality display using a focus-tunable lens.

PubMed

Shen, Xin; Javidi, Bahram

2018-03-01

We have developed a three-dimensional (3D) dynamic integral-imaging (InIm)-system-based optical see-through augmented reality display with enhanced depth range of a 3D augmented image. A focus-tunable lens is adopted in the 3D display unit to relay the elemental images with various positions to the micro lens array. Based on resolution priority integral imaging, multiple lenslet image planes are generated to enhance the depth range of the 3D image. The depth range is further increased by utilizing both the real and virtual 3D imaging fields. The 3D reconstructed image and the real-world scene are overlaid using an optical see-through display for augmented reality. The proposed system can significantly enhance the depth range of a 3D reconstructed image with high image quality in the micro InIm unit. This approach provides enhanced functionality for augmented information and adjusts the vergence-accommodation conflict of a traditional augmented reality display.

Three-dimensional label-free imaging and quantification of lipid droplets in live hepatocytes

NASA Astrophysics Data System (ADS)

Kim, Kyoohyun; Lee, Seoeun; Yoon, Jonghee; Heo, Jihan; Choi, Chulhee; Park, Yongkeun

2016-11-01

Lipid droplets (LDs) are subcellular organelles with important roles in lipid storage and metabolism and involved in various diseases including cancer, obesity, and diabetes. Conventional methods, however, have limited ability to provide quantitative information on individual LDs and have limited capability for three-dimensional (3-D) imaging of LDs in live cells especially for fast acquisition of 3-D dynamics. Here, we present an optical method based on 3-D quantitative phase imaging to measure the 3-D structural distribution and biochemical parameters (concentration and dry mass) of individual LDs in live cells without using exogenous labelling agents. The biochemical change of LDs under oleic acid treatment was quantitatively investigated, and 4-D tracking of the fast dynamics of LDs revealed the intracellular transport of LDs in live cells.

InP-based Geiger-mode avalanche photodiode arrays for three-dimensional imaging at 1.06 μm

NASA Astrophysics Data System (ADS)

Itzler, Mark A.; Entwistle, Mark; Owens, Mark; Jiang, Xudong; Patel, Ketan; Slomkowski, Krystyna; Koch, Tim; Rangwala, Sabbir; Zalud, Peter F.; Yu, Young; Tower, John; Ferraro, Joseph

2009-05-01

We report on the development of 32 x 32 focal plane arrays (FPAs) based on InGaAsP/InP Geiger-mode avalanche photodiodes (GmAPDs) designed for use in three-dimensional (3-D) laser radar imaging systems at 1064 nm. To our knowledge, this is the first realization of FPAs for 3-D imaging that employ a planar-passivated buried-junction InP-based GmAPD device platform. This development also included the design and fabrication of custom readout integrate circuits (ROICs) to perform avalanche detection and time-of-flight measurements on a per-pixel basis. We demonstrate photodiode arrays (PDAs) with a very narrow breakdown voltage distribution width of 0.34 V, corresponding to a breakdown voltage total variation of less than +/- 0.2%. At an excess bias voltage of 3.3 V, which provides 40% pixel-level single photon detection efficiency, we achieve average dark count rates of 2 kHz at an operating temperature of 248 K. We present the characterization of optical crosstalk induced by hot carrier luminescence during avalanche events, where we show that the worst-case crosstalk probability per pixel, which occurs for nearest neighbors, has a value of less than 1.6% and exhibits anisotropy due to isolation trench etch geometry. To demonstrate the FPA response to optical density variations, we show a simple image of a broadened optical beam.

Plane-Based Sampling for Ray Casting Algorithm in Sequential Medical Images

PubMed Central

Lin, Lili; Chen, Shengyong; Shao, Yan; Gu, Zichun

2013-01-01

This paper proposes a plane-based sampling method to improve the traditional Ray Casting Algorithm (RCA) for the fast reconstruction of a three-dimensional biomedical model from sequential images. In the novel method, the optical properties of all sampling points depend on the intersection points when a ray travels through an equidistant parallel plan cluster of the volume dataset. The results show that the method improves the rendering speed at over three times compared with the conventional algorithm and the image quality is well guaranteed. PMID:23424608

Imaging three-dimensional light propagation through periodic nanohole arrays using scanning aperture microscopy

PubMed Central

Chowdhury, Mustafa H.; Catchmark, Jeffrey M.; Lakowicz, Joseph R.

2009-01-01

The authors introduce a technique for three-dimensional (3D) imaging of the light transmitted through periodic nanoapertures using a scanning probe to perform optical sectioning microscopy. For a 4×4 nanohole array, the transmitted light displays intensity modulations along the propagation axis, with the maximum intensity occurring at 450 μm above the surface. The propagating fields show low divergence, suggesting a beaming effect induced by the array. At distances within 25 μm from the surface, they observe subwavelength confinement of light propagating from the individual nanoholes. Hence, this technique can potentially be used to map the 3D distribution of propagating light, with high spatial resolution. PMID:19696912

Moire measuring technology for three-dimensional profile of the object

NASA Astrophysics Data System (ADS)

Fu, Yanjun; Yang, Kuntao

2006-02-01

An optical system is designed to get projection of the transmission grating, the deformed grating is obtained on surface of the object. The image of the deformed grating is given by the lens, the reference grating is put on the place of the image, and then the moire fringe is obtained. The amplify principle of the moire fringe is used to measure the profile of the object. The optical principle of the projection is analyzed. And the relation between the phase and the height of object is deduced. From the different point of geometry optics and the physics opticsl, the optical system is analyzed, the factors that influence the image equality and the measuring result are obtained. So the betterment of improving the measuring precision is brought forward, and in the later information processing, because of the diffuse reflection, the image equality is not very well. In order to get a good image, the digital filter is used to filter the noise and smooth the image firstly. Then in order to improve the measure precision, the subdivision technology is applied. The Fourier transform profilometry and phase shifting technology is used in the calculation. A detail analyses is done both in time field and frequency field. And the method of improving the measuring precision is put forward. A good digital filter algorithm is brought forward in the Fourier transform profilometry. In the phase shifting technology, the detail formula of three-step and four-step is given. At last the phase that is relational with the high information of the object is get, but the phase is disconnected phase, after the unwrapping algorithm,the disconnected phase is changed to be the continuous phase. Taking use of the relation between the phase and height, the height is obtained. Then the three-dimensional profile of the measured object can be reconstructed. The system is very convenient for non-contact measure of profile of some objects.

A Matlab toolkit for three-dimensional electrical impedance tomography: a contribution to the Electrical Impedance and Diffuse Optical Reconstruction Software project

NASA Astrophysics Data System (ADS)

Polydorides, Nick; Lionheart, William R. B.

2002-12-01

The objective of the Electrical Impedance and Diffuse Optical Reconstruction Software project is to develop freely available software that can be used to reconstruct electrical or optical material properties from boundary measurements. Nonlinear and ill posed problems such as electrical impedance and optical tomography are typically approached using a finite element model for the forward calculations and a regularized nonlinear solver for obtaining a unique and stable inverse solution. Most of the commercially available finite element programs are unsuitable for solving these problems because of their conventional inefficient way of calculating the Jacobian, and their lack of accurate electrode modelling. A complete package for the two-dimensional EIT problem was officially released by Vauhkonen et al at the second half of 2000. However most industrial and medical electrical imaging problems are fundamentally three-dimensional. To assist the development we have developed and released a free toolkit of Matlab routines which can be employed to solve the forward and inverse EIT problems in three dimensions based on the complete electrode model along with some basic visualization utilities, in the hope that it will stimulate further development. We also include a derivation of the formula for the Jacobian (or sensitivity) matrix based on the complete electrode model.

Time-dependent photon migration imaging

NASA Astrophysics Data System (ADS)

Sevick, Eva M.; Wang, NaiGuang; Chance, Britton

1992-02-01

Recently, the application of both time- and frequency-resolved fluorescence techniques for the determination of photon migration characteristics in strongly scattering media has been used to characterize the optical properties in strongly scattering media. Specifically, Chance and coworkers have utilized measurement of photon migration characteristics to determine tissue hemoglobin absorbance and ultimately oxygenation status in homogeneous tissues. In this study, we present simulation results and experimental measurements for both techniques to show the capacity of time-dependent photon migration characteristics to image optically obscure absorbers located in strongly scattering media. The applications of time-dependent photon imaging in the biomedical community include imaging of light absorbing hematomas, tumors, hypoxic tissue volumes, and other tissue abnormalities. Herein, we show that the time-resolved parameter of mean photon path length, , and the frequency- resolved parameter of phase-shift, (theta) , can be used similarly to obtain three dimensional information of absorber position from two-dimensional measurements. Finally, we show that unlike imaging techniques that monitor the intensity of light without regard to the migration characteristics, the resolution of time-dependent photon migration measurements is enhanced by tissue scattering, further potentiating their use for biomedical imaging.

Four dimensional hybrid ultrasound and optoacoustic imaging via passive element optical excitation in a hand-held probe

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fehm, Thomas Felix; Razansky, Daniel, E-mail: dr@tum.de; Faculty of Medicine, Technische Universität München, Munich

2014-10-27

Ultrasonography and optoacoustic imaging share powerful advantages related to the natural aptitude for real-time image rendering with high resolution, the hand-held operation, and lack of ionizing radiation. The two methods also possess very different yet highly complementary advantages of the mechanical and optical contrast in living tissues. Nonetheless, efficient integration of these modalities remains challenging owing to the fundamental differences in the underlying physical contrast, optimal signal acquisition, and image reconstruction approaches. We report on a method for hybrid acquisition and reconstruction of three-dimensional pulse-echo ultrasound and optoacoustic images in real time based on passive ultrasound generation with an opticalmore » absorber, thus avoiding the hardware complexity of active ultrasound generation. In this way, complete hybrid datasets are generated with a single laser interrogation pulse, resulting in simultaneous rendering of ultrasound and optoacoustic images at an unprecedented rate of 10 volumetric frames per second. Performance is subsequently showcased in phantom experiments and in-vivo measurements from a healthy human volunteer, confirming general clinical applicability of the method.« less

Single camera volumetric velocimetry in aortic sinus with a percutaneous valve

NASA Astrophysics Data System (ADS)

Clifford, Chris; Thurow, Brian; Midha, Prem; Okafor, Ikechukwu; Raghav, Vrishank; Yoganathan, Ajit

2016-11-01

Cardiac flows have long been understood to be highly three dimensional, yet traditional in vitro techniques used to capture these complexities are costly and cumbersome. Thus, two dimensional techniques are primarily used for heart valve flow diagnostics. The recent introduction of plenoptic camera technology allows for traditional cameras to capture both spatial and angular information from a light field through the addition of a microlens array in front of the image sensor. When combined with traditional particle image velocimetry (PIV) techniques, volumetric velocity data may be acquired with a single camera using off-the-shelf optics. Particle volume pairs are reconstructed from raw plenoptic images using a filtered refocusing scheme, followed by three-dimensional cross-correlation. This technique was applied to the sinus region (known for having highly three-dimensional flow structures) of an in vitro aortic model with a percutaneous valve. Phase-locked plenoptic PIV data was acquired at two cardiac outputs (2 and 5 L/min) and 7 phases of the cardiac cycle. The volumetric PIV data was compared to standard 2D-2C PIV. Flow features such as recirculation and stagnation were observed in the sinus region in both cases.

Detection of amblyopia utilizing generated retinal reflexes

NASA Technical Reports Server (NTRS)

Kerr, J. H.; Hay, S. H.

1981-01-01

Investigation confirmed that GRR images can be consistently obtained and that these images contain information required to detect the optical inequality of one eye compared to the fellow eye. Digital analyses, electro-optical analyses, and trained observers were used to evaluate the GRR images. Two and three dimensional plots were made from the digital analyses results. These plotted data greatly enhanced the GRR image content, and it was possible for nontrained observers to correctly identify normal vs abnormal ocular status by viewing the plots. Based upon the criteria of detecting equality or inequality of ocular status of a person's eyes, the trained observer correctly identified the ocular status of 90% of the 232 persons who participated in this program.

A system and methodology for high-content visual screening of individual intact living cells in suspension

NASA Astrophysics Data System (ADS)

Renaud, Olivier; Heintzmann, Rainer; Sáez-Cirión, Asier; Schnelle, Thomas; Mueller, Torsten; Shorte, Spencer

2007-02-01

Three dimensional imaging provides high-content information from living intact biology, and can serve as a visual screening cue. In the case of single cell imaging the current state of the art uses so-called "axial through-stacking". However, three-dimensional axial through-stacking requires that the object (i.e. a living cell) be adherently stabilized on an optically transparent surface, usually glass; evidently precluding use of cells in suspension. Aiming to overcome this limitation we present here the utility of dielectric field trapping of single cells in three-dimensional electrode cages. Our approach allows gentle and precise spatial orientation and vectored rotation of living, non-adherent cells in fluid suspension. Using various modes of widefield, and confocal microscope imaging we show how so-called "microrotation" can provide a unique and powerful method for multiple point-of-view (three-dimensional) interrogation of intact living biological micro-objects (e.g. single-cells, cell aggregates, and embryos). Further, we show how visual screening by micro-rotation imaging can be combined with micro-fluidic sorting, allowing selection of rare phenotype targets from small populations of cells in suspension, and subsequent one-step single cell cloning (with high-viability). Our methodology combining high-content 3D visual screening with one-step single cell cloning, will impact diverse paradigms, for example cytological and cytogenetic analysis on haematopoietic stem cells, blood cells including lymphocytes, and cancer cells.

Volumetric imaging of rod and cone photoreceptor structure with a combined adaptive optics-optical coherence tomography-scanning laser ophthalmoscope

NASA Astrophysics Data System (ADS)

Wells-Gray, Elaine M.; Choi, Stacey S.; Zawadzki, Robert J.; Finn, Susanna C.; Greiner, Cherry; Werner, John S.; Doble, Nathan

2018-03-01

We have designed and implemented a dual-mode adaptive optics (AO) imaging system that combines spectral domain optical coherence tomography (OCT) and scanning laser ophthalmoscopy (SLO) for in vivo imaging of the human retina. The system simultaneously acquires SLO frames and OCT B-scans at 60 Hz with an OCT volume acquisition time of 4.2 s. Transverse eye motion measured from the SLO is used to register the OCT B-scans to generate three-dimensional (3-D) volumes. Key optical design considerations include: minimizing system aberrations through the use of off-axis relay telescopes, conjugate pupil plane requirements, and the use of dichroic beam splitters to separate and recombine the OCT and SLO beams around the nonshared horizontal scanning mirrors. To demonstrate system performance, AO-OCT-SLO images and measurements are taken from three normal human subjects ranging in retinal eccentricity from the fovea out to 15-deg temporal and 20-deg superior. Also presented are en face OCT projections generated from the registered 3-D volumes. The ability to acquire high-resolution 3-D images of the human retina in the midperiphery and beyond has clinical importance in diseases, such as retinitis pigmentosa and cone-rod dystrophy.

Three-dimensional wide-field pump-probe structured illumination microscopy

PubMed Central

Kim, Yang-Hyo; So, Peter T.C.

2017-01-01

We propose a new structured illumination scheme for achieving depth resolved wide-field pump-probe microscopy with sub-diffraction limit resolution. By acquiring coherent pump-probe images using a set of 3D structured light illumination patterns, a 3D super-resolution pump-probe image can be reconstructed. We derive the theoretical framework to describe the coherent image formation and reconstruction scheme for this structured illumination pump-probe imaging system and carry out numerical simulations to investigate its imaging performance. The results demonstrate a lateral resolution improvement by a factor of three and providing 0.5 µm level axial optical sectioning. PMID:28380860

Simultaneous three-dimensional laser-ultrasound and photoacoustic imaging

NASA Astrophysics Data System (ADS)

Wurzinger, Gerhild; Nuster, Robert; Schmitner, Nicole; Gratt, Sibylle; Paltauf, Günther

2013-06-01

A purely optical setup for simultaneous photoacoustic (PA) and laser-ultrasound (US) tomography is presented. It is shown that combined imaging can be achieved by using the same laser pulse for photoacoustic generation and for launching a broadband ultrasound pulse from an optically absorbing target. Detection of the laser-generated plane waves that have been scattered at the imaging object and of the photoacoustic signals emitted from the sample is done interferometrically. This way data for PA and US imaging are acquired within one single measurement. Distinction between the signals is possible due to their different times of flight. After data separation, image reconstruction is done using standard back-projection algorithms. The resolution of the setup was estimated and images of a zebra fish are shown, demonstrating the complementary information of the two imaging modalities.

Three-dimensional optical topography of brain activity in infants watching videos of human movement

NASA Astrophysics Data System (ADS)

Correia, Teresa; Lloyd-Fox, Sarah; Everdell, Nick; Blasi, Anna; Elwell, Clare; Hebden, Jeremy C.; Gibson, Adam

2012-03-01

We present 3D optical topography images reconstructed from data obtained previously while infants observed videos of adults making natural movements of their eyes and hands. The optical topography probe was placed over the temporal cortex, which in adults is responsible for cognitive processing of similar stimuli. Increases in oxyhaemoglobin were measured and reconstructed using a multispectral imaging algorithm with spatially variant regularization to optimize depth discrimination. The 3D optical topography images suggest that similar brain regions are activated in infants and adults. Images were presented showing the distribution of activation in a plane parallel to the surface, as well as changes in activation with depth. The time-course of activation was followed in the pixel which demonstrated the largest change, showing that changes could be measured with high temporal resolution. These results suggest that infants a few months old have regions which are specialized for reacting to human activity, and that these subtle changes can be effectively analysed using 3D optical topography.

Hybrid numerical method for solution of the radiative transfer equation in one, two, or three dimensions.

PubMed

Reinersman, Phillip N; Carder, Kendall L

2004-05-01

A hybrid method is presented by which Monte Carlo (MC) techniques are combined with an iterative relaxation algorithm to solve the radiative transfer equation in arbitrary one-, two-, or three-dimensional optical environments. The optical environments are first divided into contiguous subregions, or elements. MC techniques are employed to determine the optical response function of each type of element. The elements are combined, and relaxation techniques are used to determine simultaneously the radiance field on the boundary and throughout the interior of the modeled environment. One-dimensional results compare well with a standard radiative transfer model. The light field beneath and adjacent to a long barge is modeled in two dimensions and displayed. Ramifications for underwater video imaging are discussed. The hybrid model is currently capable of providing estimates of the underwater light field needed to expedite inspection of ship hulls and port facilities.

Defining the uncertainty of electro-optical identification system performance estimates using a 3D optical environment derived from satellite

NASA Astrophysics Data System (ADS)

Ladner, S. D.; Arnone, R.; Casey, B.; Weidemann, A.; Gray, D.; Shulman, I.; Mahoney, K.; Giddings, T.; Shirron, J.

2009-05-01

Current United States Navy Mine-Counter-Measure (MCM) operations primarily use electro-optical identification (EOID) sensors to identify underwater targets after detection via acoustic sensors. These EOID sensors which are based on laser underwater imaging by design work best in "clear" waters and are limited in coastal waters especially with strong optical layers. Optical properties and in particular scattering and absorption play an important role on systems performance. Surface optical properties alone from satellite are not adequate to determine how well a system will perform at depth due to the existence of optical layers. The spatial and temporal characteristics of the 3d optical variability of the coastal waters along with strength and location of subsurface optical layers maximize chances of identifying underwater targets by exploiting optimum sensor deployment. Advanced methods have been developed to fuse the optical measurements from gliders, optical properties from "surface" satellite snapshot and 3-D ocean circulation models to extend the two-dimensional (2-D) surface satellite optical image into a three-dimensional (3-D) optical volume with subsurface optical layers. Modifications were made to an EOID performance model to integrate a 3-D optical volume covering an entire region of interest as input and derive system performance field. These enhancements extend present capability based on glider optics and EOID sensor models to estimate the system's "image quality". This only yields system performance information for a single glider profile location in a very large operational region. Finally, we define the uncertainty of the system performance by coupling the EOID performance model with the 3-D optical volume uncertainties. Knowing the ensemble spread of EOID performance field provides a new and unique capability for tactical decision makers and Navy Operations.

Optimized SIFTFlow for registration of whole-mount histology to reference optical images

PubMed Central

Shojaii, Rushin; Martel, Anne L.

2016-01-01

Abstract. The registration of two-dimensional histology images to reference images from other modalities is an important preprocessing step in the reconstruction of three-dimensional histology volumes. This is a challenging problem because of the differences in the appearances of histology images and other modalities, and the presence of large nonrigid deformations which occur during slide preparation. This paper shows the feasibility of using densely sampled scale-invariant feature transform (SIFT) features and a SIFTFlow deformable registration algorithm for coregistering whole-mount histology images with blockface optical images. We present a method for jointly optimizing the regularization parameters used by the SIFTFlow objective function and use it to determine the most appropriate values for the registration of breast lumpectomy specimens. We demonstrate that tuning the regularization parameters results in significant improvements in accuracy and we also show that SIFTFlow outperforms a previously described edge-based registration method. The accuracy of the histology images to blockface images registration using the optimized SIFTFlow method was assessed using an independent test set of images from five different lumpectomy specimens and the mean registration error was 0.32±0.22  mm. PMID:27774494

Three-Dimensional Optical Mapping of Nanoparticle Distribution in Intact Tissues.

PubMed

Sindhwani, Shrey; Syed, Abdullah Muhammad; Wilhelm, Stefan; Glancy, Dylan R; Chen, Yih Yang; Dobosz, Michael; Chan, Warren C W

2016-05-24

The role of tissue architecture in mediating nanoparticle transport, targeting, and biological effects is unknown due to the lack of tools for imaging nanomaterials in whole organs. Here, we developed a rapid optical mapping technique to image nanomaterials in intact organs ex vivo and in three-dimensions (3D). We engineered a high-throughput electrophoretic flow device to simultaneously transform up to 48 tissues into optically transparent structures, allowing subcellular imaging of nanomaterials more than 1 mm deep into tissues which is 25-fold greater than current techniques. A key finding is that nanomaterials can be retained in the processed tissue by chemical cross-linking of surface adsorbed serum proteins to the tissue matrix, which enables nanomaterials to be imaged with respect to cells, blood vessels, and other structures. We developed a computational algorithm to analyze and quantitatively map nanomaterial distribution. This method can be universally applied to visualize the distribution and interactions of materials in whole tissues and animals including such applications as the imaging of nanomaterials, tissue engineered constructs, and biosensors within their intact biological environment.

Speckle-based three-dimensional velocity measurement using spatial filtering velocimetry.

PubMed

Iversen, Theis F Q; Jakobsen, Michael L; Hanson, Steen G

2011-04-10

We present an optical method for measuring the real-time three-dimensional (3D) translational velocity of a diffusely scattering rigid object observed through an imaging system. The method is based on a combination of the motion of random speckle patterns and regular fringe patterns. The speckle pattern is formed in the observation plane of the imaging system due to reflection from an area of the object illuminated by a coherent light source. The speckle pattern translates in response to in-plane translation of the object, and the presence of an angular offset reference wave coinciding with the speckle pattern in the observation plane gives rise to interference, resulting in a fringe pattern that translates in response to the out-of-plane translation of the object. Numerical calculations are performed to evaluate the dynamic properties of the intensity distribution and the response of realistic spatial filters designed to measure the three components of the object's translational velocity. Furthermore, experimental data are presented that demonstrate full 3D velocity measurement. © 2011 Optical Society of America

Second-Generation System for Three-Dimensional Imaging Using a Single Laser Pulse

DTIC Science & Technology

1990-01-23

Welford and t. Winston , The Optics of Nonimaging Concentrators , Academic Press, New York, 1978. 14. N. F. Borelli, D. L. Morse, R. H. Bellman, and W. L...inserting such an optical concentrator , we can use a practical- 9ber layout for the converter with moderate ratio of fiber core to unit cell area and...ABSTRACT (Maximum 200 words)15 199t This paper describes the design of a second-generation version of an optical detector capable of producing a 3-D

Three Dimensional Reconstruction Algorithm for Imaging Pathophysiological Signals Within Breast Tissue Using Near Infrared Light

DTIC Science & Technology

2006-07-01

of water, gelatin (G2625, Sigma Inc.), India ink (for absorption), and titanium dioxide powder (for scatter) (TiO2, Sigma Inc.) is poured into a mold...R. C., Ference, R. J, Refractive index of some mammalian tissue using a fiber optic cladding method. Applied Optics, 1989. 28(12): p. 2297-2303. 3...scans. The NIR system utilizes six optical wavelengths from 660 to 850 nm using intensity modulated diode lasers nominally working at 100 MHz

Holographic motion picture camera with Doppler shift compensation

NASA Technical Reports Server (NTRS)

Kurtz, R. L. (Inventor)

1976-01-01

A holographic motion picture camera is reported for producing three dimensional images by employing an elliptical optical system. There is provided in one of the beam paths (the object or reference beam path) a motion compensator which enables the camera to photograph faster moving objects.

Anatomic Optical Coherence Tomography of Upper Airways

NASA Astrophysics Data System (ADS)

Chin Loy, Anthony; Jing, Joseph; Zhang, Jun; Wang, Yong; Elghobashi, Said; Chen, Zhongping; Wong, Brian J. F.

The upper airway is a complex and intricate system responsible for respiration, phonation, and deglutition. Obstruction of the upper airways afflicts an estimated 12-18 million Americans. Pharyngeal size and shape are important factors in the pathogenesis of airway obstructions. In addition, nocturnal loss in pharyngeal muscular tone combined with high pharyngeal resistance can lead to collapse of the airway and periodic partial or complete upper airway obstruction. Anatomical optical coherence tomography (OCT) has the potential to provide high-speed three-dimensional tomographic images of the airway lumen without the use of ionizing radiation. In this chapter we describe the methods behind endoscopic OCT imaging and processing to generate full three dimensional anatomical models of the human airway which can be used in conjunction with numerical simulation methods to assess areas of airway obstruction. Combining this structural information with flow dynamic simulations, we can better estimate the site and causes of airway obstruction and better select and design surgery for patients with obstructive sleep apnea.

Partially coherent lensfree tomographic microscopy⋄

PubMed Central

Isikman, Serhan O.; Bishara, Waheb; Ozcan, Aydogan

2012-01-01

Optical sectioning of biological specimens provides detailed volumetric information regarding their internal structure. To provide a complementary approach to existing three-dimensional (3D) microscopy modalities, we have recently demonstrated lensfree optical tomography that offers high-throughput imaging within a compact and simple platform. In this approach, in-line holograms of objects at different angles of partially coherent illumination are recorded using a digital sensor-array, which enables computing pixel super-resolved tomographic images of the specimen. This imaging modality, which forms the focus of this review, offers micrometer-scale 3D resolution over large imaging volumes of, for example, 10–15 mm3, and can be assembled in light weight and compact architectures. Therefore, lensfree optical tomography might be particularly useful for lab-on-a-chip applications as well as for microscopy needs in resource-limited settings. PMID:22193016

Three-dimensional surface contouring of macroscopic objects by means of phase-difference images.

PubMed

Velásquez Prieto, Daniel; Garcia-Sucerquia, Jorge

2006-09-01

We report a technique to determine the 3D contour of objects with dimensions of at least 4 orders of magnitude larger than the illumination optical wavelength. Our proposal is based on the numerical reconstruction of the optical wave field of digitally recorded holograms. The required modulo 2pi phase map in any contouring process is obtained by means of the direct subtraction of two phase-contrast images under different illumination angles to create a phase-difference image of a still object. Obtaining the phase-difference images is only possible by using the capability of numerical reconstruction of the complex optical field provided by digital holography. This unique characteristic leads us to a robust, reliable, and fast procedure that requires only two images. A theoretical analysis of the contouring system is shown, with verification by means of numerical and experimental results.

Textural analysis of optical coherence tomography skin images: quantitative differentiation between healthy and cancerous tissues

NASA Astrophysics Data System (ADS)

Adabi, Saba; Conforto, Silvia; Hosseinzadeh, Matin; Noe, Shahryar; Daveluy, Steven; Mehregan, Darius; Nasiriavanaki, Mohammadreza

2017-02-01

Optical Coherence Tomography (OCT) offers real-time high-resolution three-dimensional images of tissue microstructures. In this study, we used OCT skin images acquired from ten volunteers, neither of whom had any skin conditions addressing the features of their anatomic location. OCT segmented images are analyzed based on their optical properties (attenuation coefficient) and textural image features e.g., contrast, correlation, homogeneity, energy, entropy, etc. Utilizing the information and referring to their clinical insight, we aim to make a comprehensive computational model for the healthy skin. The derived parameters represent the OCT microstructural morphology and might provide biological information for generating an atlas of normal skin from different anatomic sites of human skin and may allow for identification of cell microstructural changes in cancer patients. We then compared the parameters of healthy samples with those of abnormal skin and classified them using a linear Support Vector Machines (SVM) with 82% accuracy.

Microwave Imaging in Large Helical Device

NASA Astrophysics Data System (ADS)

Yoshinaga, T.; Nagayama, Y.; Tsuchiya, H.; Kuwahara, D.; Tsuji-Iio, S.; Akaki, K.; Mase, A.; Kogi, Y.; Yamaguchi, S.; Shi, Z. B.; Hojo, H.

2011-02-01

Microwave imaging reflectometry (MIR) system and electron cyclotron emission imaging (ECEI) system are under development for the simultaneous reconstruction of the electron density and temperature fluctuation structures in the Large Helical Device (LHD). The MIR observes three-dimensional structure of disturbed cutoff surfaces by using the two-dimensionally distributed horn-antenna mixer array (HMA) of 5 × 7 channels in combination with the simultaneous projection of microwaves with four different frequency components (60.410, 61.808, 63.008 and 64.610 GHz). The ECEI is designed to observe two-dimensional structure of electron temperature by detecting second-harmonic ECE at 97-107 GHz with the one-dimensional HMA (7 channels) in the common optics with MIR system. Both the MIR and the ECEI are realized by the HMA and the band-pass filter (BPF) arrays, which are fabricated by micro-strip-line technique at low-cost.

Two-dimensional vacuum ultraviolet images in different MHD events on the EAST tokamak

NASA Astrophysics Data System (ADS)

Zhijun, WANG; Xiang, GAO; Tingfeng, MING; Yumin, WANG; Fan, ZHOU; Feifei, LONG; Qing, ZHUANG; EAST Team

2018-02-01

A high-speed vacuum ultraviolet (VUV) imaging telescope system has been developed to measure the edge plasma emission (including the pedestal region) in the Experimental Advanced Superconducting Tokamak (EAST). The key optics of the high-speed VUV imaging system consists of three parts: an inverse Schwarzschild-type telescope, a micro-channel plate (MCP) and a visible imaging high-speed camera. The VUV imaging system has been operated routinely in the 2016 EAST experiment campaign. The dynamics of the two-dimensional (2D) images of magnetohydrodynamic (MHD) instabilities, such as edge localized modes (ELMs), tearing-like modes and disruptions, have been observed using this system. The related VUV images are presented in this paper, and it indicates the VUV imaging system is a potential tool which can be applied successfully in various plasma conditions.

Accuracy of neuro-navigated cranial screw placement using optical surface imaging (Conference Presentation)

NASA Astrophysics Data System (ADS)

Jakubovic, Raphael; Gupta, Shuarya; Guha, Daipayan; Mainprize, Todd; Yang, Victor X. D.

2017-02-01

Cranial neurosurgical procedures are especially delicate considering that the surgeon must localize the subsurface anatomy with limited exposure and without the ability to see beyond the surface of the surgical field. Surgical accuracy is imperative as even minor surgical errors can cause major neurological deficits. Traditionally surgical precision was highly dependent on surgical skill. However, the introduction of intraoperative surgical navigation has shifted the paradigm to become the current standard of care for cranial neurosurgery. Intra-operative image guided navigation systems are currently used to allow the surgeon to visualize the three-dimensional subsurface anatomy using pre-acquired computed tomography (CT) or magnetic resonance (MR) images. The patient anatomy is fused to the pre-acquired images using various registration techniques and surgical tools are typically localized using optical tracking methods. Although these techniques positively impact complication rates, surgical accuracy is limited by the accuracy of the navigation system and as such quantification of surgical error is required. While many different measures of registration accuracy have been presented true navigation accuracy can only be quantified post-operatively by comparing a ground truth landmark to the intra-operative visualization. In this study we quantified the accuracy of cranial neurosurgical procedures using a novel optical surface imaging navigation system to visualize the three-dimensional anatomy of the surface anatomy. A tracked probe was placed on the screws of cranial fixation plates during surgery and the reported position of the centre of the screw was compared to the co-ordinates of the post-operative CT or MR images, thus quantifying cranial neurosurgical error.

Practical guidelines for implementing adaptive optics in fluorescence microscopy

NASA Astrophysics Data System (ADS)

Wilding, Dean; Pozzi, Paolo; Soloviev, Oleg; Vdovin, Gleb; Verhaegen, Michel

2018-02-01

In life sciences, interest in the microscopic imaging of increasingly complex three dimensional samples, such as cell spheroids, zebrafish embryos, and in vivo applications in small animals, is growing quickly. Due to the increasing complexity of samples, more and more life scientists are considering the implementation of adaptive optics in their experimental setups. While several approaches to adaptive optics in microscopy have been reported, it is often difficult and confusing for the microscopist to choose from the array of techniques and equipment. In this poster presentation we offer a small guide to adaptive optics providing general guidelines for successful adaptive optics implementation.

Development of a three-dimensional correction method for optical distortion of flow field inside a liquid droplet.

PubMed

Gim, Yeonghyeon; Ko, Han Seo

2016-04-15

In this Letter, a three-dimensional (3D) optical correction method, which was verified by simulation, was developed to reconstruct droplet-based flow fields. In the simulation, a synthetic phantom was reconstructed using a simultaneous multiplicative algebraic reconstruction technique with three detectors positioned at the synthetic object (represented by the phantom), with offset angles of 30° relative to each other. Additionally, a projection matrix was developed using the ray tracing method. If the phantom is in liquid, the image of the phantom can be distorted since the light passes through a convex liquid-vapor interface. Because of the optical distortion effect, the projection matrix used to reconstruct a 3D field should be supplemented by the revision ray, instead of the original projection ray. The revision ray can be obtained from the refraction ray occurring on the surface of the liquid. As a result, the error on the reconstruction field of the phantom could be reduced using the developed optical correction method. In addition, the developed optical method was applied to a Taylor cone which was caused by the high voltage between the droplet and the substrate.

Dual-polarized light-field imaging micro-system via a liquid-crystal microlens array for direct three-dimensional observation.

PubMed

Xin, Zhaowei; Wei, Dong; Xie, Xingwang; Chen, Mingce; Zhang, Xinyu; Liao, Jing; Wang, Haiwei; Xie, Changsheng

2018-02-19

Light-field imaging is a crucial and straightforward way of measuring and analyzing surrounding light worlds. In this paper, a dual-polarized light-field imaging micro-system based on a twisted nematic liquid-crystal microlens array (TN-LCMLA) for direct three-dimensional (3D) observation is fabricated and demonstrated. The prototyped camera has been constructed by integrating a TN-LCMLA with a common CMOS sensor array. By switching the working state of the TN-LCMLA, two orthogonally polarized light-field images can be remapped through the functioned imaging sensors. The imaging micro-system in conjunction with the electric-optical microstructure can be used to perform polarization and light-field imaging, simultaneously. Compared with conventional plenoptic cameras using liquid-crystal microlens array, the polarization-independent light-field images with a high image quality can be obtained in the arbitrary polarization state selected. We experimentally demonstrate characters including a relatively wide operation range in the manipulation of incident beams and the multiple imaging modes, such as conventional two-dimensional imaging, light-field imaging, and polarization imaging. Considering the obvious features of the TN-LCMLA, such as very low power consumption, providing multiple imaging modes mentioned, simple and low-cost manufacturing, the imaging micro-system integrated with this kind of liquid-crystal microstructure driven electrically presents the potential capability of directly observing a 3D object in typical scattering media.

Optical biopsy of lymph node morphology using optical coherence tomography.

PubMed

Luo, Wei; Nguyen, Freddy T; Zysk, Adam M; Ralston, Tyler S; Brockenbrough, John; Marks, Daniel L; Oldenburg, Amy L; Boppart, Stephen A

2005-10-01

Optical diagnostic imaging techniques are increasingly being used in the clinical environment, allowing for improved screening and diagnosis while minimizing the number of invasive procedures. Diffuse optical tomography, for example, is capable of whole-breast imaging and is being developed as an alternative to traditional X-ray mammography. While this may eventually be a very effective screening method, other optical techniques are better suited for imaging on the cellular and molecular scale. Optical Coherence Tomography (OCT), for instance, is capable of high-resolution cross-sectional imaging of tissue morphology. In a manner analogous to ultrasound imaging except using optics, pulses of near-infrared light are sent into the tissue while coherence-gated reflections are measured interferometrically to form a cross-sectional image of tissue. In this paper we apply OCT techniques for the high-resolution three-dimensional visualization of lymph node morphology. We present the first reported OCT images showing detailed morphological structure and corresponding histological features of lymph nodes from a carcinogen-induced rat mammary tumor model, as well as from a human lymph node containing late stage metastatic disease. The results illustrate the potential for OCT to visualize detailed lymph node structures on the scale of micrometastases and the potential for the detection of metastatic nodal disease intraoperatively.

Effect of multiple circular holes Fraunhofer diffraction for the infrared optical imaging

NASA Astrophysics Data System (ADS)

Lu, Chunlian; Lv, He; Cao, Yang; Cai, Zhisong; Tan, Xiaojun

2014-11-01

With the development of infrared optics, infrared optical imaging systems play an increasingly important role in modern optical imaging systems. Infrared optical imaging is used in industry, agriculture, medical, military and transportation. But in terms of infrared optical imaging systems which are exposed for a long time, some contaminations will affect the infrared optical imaging. When the contamination contaminate on the lens surface of the optical system, it would affect diffraction. The lens can be seen as complementary multiple circular holes screen happen Fraunhofer diffraction. According to Babinet principle, you can get the diffraction of the imaging system. Therefore, by studying the multiple circular holes Fraunhofer diffraction, conclusions can be drawn about the effect of infrared imaging. This paper mainly studies the effect of multiple circular holes Fraunhofer diffraction for the optical imaging. Firstly, we introduce the theory of Fraunhofer diffraction and Point Spread Function. Point Spread Function is a basic tool to evaluate the image quality of the optical system. Fraunhofer diffraction will affect Point Spread Function. Then, the results of multiple circular holes Fraunhofer diffraction are given for different hole size and hole spacing. We choose the hole size from 0.1mm to 1mm and hole spacing from 0.3mm to 0.8mm. The infrared wavebands of optical imaging are chosen from 1μm to 5μm. We use the MATLAB to simulate light intensity distribution of multiple circular holes Fraunhofer diffraction. Finally, three-dimensional diffraction maps of light intensity are given to contrast.

Radar E-O image fusion

NASA Technical Reports Server (NTRS)

Oneil, William F.

1993-01-01

The fusion of radar and electro-optic (E-O) sensor images presents unique challenges. The two sensors measure different properties of the real three-dimensional (3-D) world. Forming the sensor outputs into a common format does not mask these differences. In this paper, the conditions under which fusion of the two sensor signals is possible are explored. The program currently planned to investigate this problem is briefly discussed.

Simulated electronic heterodyne recording and processing of pulsed-laser holograms

NASA Technical Reports Server (NTRS)

Decker, A. J.

1979-01-01

The electronic recording of pulsed-laser holograms is proposed. The polarization sensitivity of each resolution element of the detector is controlled independently to add an arbitrary phase to the image waves. This method which can be used to simulate heterodyne recording and to process three-dimensional optical images, is based on a similar method for heterodyne recording and processing of continuous-wave holograms.

Engineering of Nanoscale Contrast Agents for Optical Coherence Tomography.

PubMed

Gordon, Andrew Y; Jayagopal, Ashwath

2014-01-30

Optical coherence tomography has emerged as valuable imaging modalityin ophthalmology and other fields by enabling high-resolution three-dimensional imaging of tissue. In this paper, we review recent progress in the field of contrast-enhanced optical coherence tomography (OCT). We discuss exogenous and endogenous sources of OCT contrast, focusing on their use with standard OCT systems as well as emerging OCT-based imaging modalities. We include advances in the processing of OCT data that generate improved tissue contrast, including spectroscopic OCT (SOCT), as well as work utilizing secondary light sources and/or detection mechanisms to create and detect enhanced contrast, including photothermal OCT (PTOCT) and photoacoustic OCT (PAOCT). Finally, we conclude with a discussion of the translational potential of these developments as well as barriers to their clinical use.

Biodynamic imaging for phenotypic profiling of three-dimensional tissue culture

PubMed Central

Sun, Hao; Merrill, Daniel; An, Ran; Turek, John; Matei, Daniela; Nolte, David D.

2017-01-01

Abstract. Three-dimensional (3-D) tissue culture represents a more biologically relevant environment for testing new drugs compared to conventional two-dimensional cancer cell culture models. Biodynamic imaging is a high-content 3-D optical imaging technology based on low-coherence interferometry and digital holography that uses dynamic speckle as high-content image contrast to probe deep inside 3-D tissue. Speckle contrast is shown to be a scaling function of the acquisition time relative to the persistence time of intracellular transport and hence provides a measure of cellular activity. Cellular responses of 3-D multicellular spheroids to paclitaxel are compared among three different growth techniques: rotating bioreactor (BR), hanging-drop (HD), and nonadherent (U-bottom, UB) plate spheroids, compared with ex vivo living tissues. HD spheroids have the most homogeneous tissue, whereas BR spheroids display large sample-to-sample variability as well as spatial heterogeneity. The responses of BR-grown tumor spheroids to paclitaxel are more similar to those of ex vivo biopsies than the responses of spheroids grown using HD or plate methods. The rate of mitosis inhibition by application of taxol is measured through tissue dynamics spectroscopic imaging, demonstrating the ability to monitor antimitotic chemotherapy. These results illustrate the potential use of low-coherence digital holography for 3-D pharmaceutical screening applications. PMID:28301634

Biodynamic imaging for phenotypic profiling of three-dimensional tissue culture

NASA Astrophysics Data System (ADS)

Sun, Hao; Merrill, Daniel; An, Ran; Turek, John; Matei, Daniela; Nolte, David D.

2017-01-01

Three-dimensional (3-D) tissue culture represents a more biologically relevant environment for testing new drugs compared to conventional two-dimensional cancer cell culture models. Biodynamic imaging is a high-content 3-D optical imaging technology based on low-coherence interferometry and digital holography that uses dynamic speckle as high-content image contrast to probe deep inside 3-D tissue. Speckle contrast is shown to be a scaling function of the acquisition time relative to the persistence time of intracellular transport and hence provides a measure of cellular activity. Cellular responses of 3-D multicellular spheroids to paclitaxel are compared among three different growth techniques: rotating bioreactor (BR), hanging-drop (HD), and nonadherent (U-bottom, UB) plate spheroids, compared with ex vivo living tissues. HD spheroids have the most homogeneous tissue, whereas BR spheroids display large sample-to-sample variability as well as spatial heterogeneity. The responses of BR-grown tumor spheroids to paclitaxel are more similar to those of ex vivo biopsies than the responses of spheroids grown using HD or plate methods. The rate of mitosis inhibition by application of taxol is measured through tissue dynamics spectroscopic imaging, demonstrating the ability to monitor antimitotic chemotherapy. These results illustrate the potential use of low-coherence digital holography for 3-D pharmaceutical screening applications.

Three-dimensional image signals: processing methods

NASA Astrophysics Data System (ADS)

Schiopu, Paul; Manea, Adrian; Craciun, Anca-Ileana; Craciun, Alexandru

2010-11-01

Over the years extensive studies have been carried out to apply coherent optics methods in real-time processing, communications and transmission image. This is especially true when a large amount of information needs to be processed, e.g., in high-resolution imaging. The recent progress in data-processing networks and communication systems has considerably increased the capacity of information exchange. We describe the results of literature investigation research of processing methods for the signals of the three-dimensional images. All commercially available 3D technologies today are based on stereoscopic viewing. 3D technology was once the exclusive domain of skilled computer-graphics developers with high-end machines and software. The images capture from the advanced 3D digital camera can be displayed onto screen of the 3D digital viewer with/ without special glasses. For this is needed considerable processing power and memory to create and render the complex mix of colors, textures, and virtual lighting and perspective necessary to make figures appear three-dimensional. Also, using a standard digital camera and a technique called phase-shift interferometry we can capture "digital holograms." These are holograms that can be stored on computer and transmitted over conventional networks. We present some research methods to process "digital holograms" for the Internet transmission and results.

Three-dimensional dynamics of temperature fields in phantoms and biotissue under IR laser photothermal therapy using gold nanoparticles and ICG dye

NASA Astrophysics Data System (ADS)

Akchurin, Georgy G.; Garif, Akchurin G.; Maksimova, Irina L.; Skaptsov, Alexander A.; Terentyuk, Georgy S.; Khlebtsov, Boris N.; Khlebtsov, Nikolai G.; Tuchin, Valery V.

2010-02-01

We describe applications of silica (core)/gold (shell) nanoparticles and ICG dye to photothermal treatment of phantoms, biotissue and spontaneous tumor of cats and dogs. The laser irradiation parameters were optimized by preliminary experiments with laboratory rats. Three dimensional dynamics of temperature fields in tissue and solution samples was measured with a thermal imaging system. It is shown that the temperature in the volume region of nanoparticles localization can substantially exceed the surface temperature recorded by the thermal imaging system. We have demonstrated effective optical destruction of cancer cells by local injection of plasmon-resonant gold nanoshells and ICG dye followed by continuous wave (CW) diode laser irradiation at wavelength 808 nm.

Nanoscopic imaging of thick heterogeneous soft-matter structures in aqueous solution

PubMed Central

Bartsch, Tobias F.; Kochanczyk, Martin D.; Lissek, Emanuel N.; Lange, Janina R.; Florin, Ernst-Ludwig

2016-01-01

Precise nanometre-scale imaging of soft structures at room temperature poses a major challenge to any type of microscopy because fast thermal fluctuations lead to significant motion blur if the position of the structure is measured with insufficient bandwidth. Moreover, precise localization is also affected by optical heterogeneities, which lead to deformations in the imaged local geometry, the severity depending on the sample and its thickness. Here we introduce quantitative thermal noise imaging, a three-dimensional scanning probe technique, as a method for imaging soft, optically heterogeneous and porous matter with submicroscopic spatial resolution in aqueous solution. By imaging both individual microtubules and collagen fibrils in a network, we demonstrate that structures can be localized with a precision of ∼10 nm and that their local dynamics can be quantified with 50 kHz bandwidth and subnanometre amplitudes. Furthermore, we show how image distortions caused by optically dense structures can be corrected for. PMID:27596919

Compton imaging tomography for nondestructive evaluation of spacecraft thermal protection systems

NASA Astrophysics Data System (ADS)

Romanov, Volodymyr; Burke, Eric; Grubsky, Victor

2017-02-01

Here we present new results of in situ nondestructive evaluation (NDE) of spacecraft thermal protection system materials obtained with POC-developed NDE tool based on a novel Compton Imaging Tomography (CIT) technique recently pioneered and patented by Physical Optics Corporation (POC). In general, CIT provides high-resolution three-dimensional Compton scattered X-ray imaging of the internal structure of evaluated objects, using a set of acquired two-dimensional Compton scattered X-ray images of consecutive cross sections of these objects. Unlike conventional computed tomography, CIT requires only one-sided access to objects, has no limitation on the dimensions and geometry of the objects, and can be applied to large multilayer non-uniform objects with complicated geometries. Also, CIT does not require any contact with the objects being imaged during its application.

Super-resolution three-dimensional fluorescence and optical diffraction tomography of live cells using structured illumination generated by a digital micromirror device.

PubMed

Shin, Seungwoo; Kim, Doyeon; Kim, Kyoohyun; Park, YongKeun

2018-06-15

We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM). A digital micromirror device is utilized to generate structured illumination patterns for both ODT and SIM, which enables fast and stable measurements. To verify its feasibility and applicability, the proposed method is used to measure the 3D RI distribution and 3D fluorescence image of various samples, including a cluster of fluorescent beads, and the time-lapse 3D RI dynamics of fluorescent beads inside a HeLa cell, from which the trajectory of the beads in the HeLa cell is analyzed using spatiotemporal correlations.

Three Dimensional Optical Coherence Tomography Imaging: Advantages and Advances

PubMed Central

Gabriele, Michelle L; Wollstein, Gadi; Ishikawa, Hiroshi; Xu, Juan; Kim, Jongsick; Kagemann, Larry; Folio, Lindsey S; Schuman, Joel S.

2010-01-01

Three dimensional (3D) ophthalmic imaging using optical coherence tomography (OCT) has revolutionized assessment of the eye, the retina in particular. Recent technological improvements have made the acquisition of 3D-OCT datasets feasible. However, while volumetric data can improve disease diagnosis and follow-up, novel image analysis techniques are now necessary in order to process the dense 3D-OCT dataset. Fundamental software improvements include methods for correcting subject eye motion, segmenting structures or volumes of interest, extracting relevant data post hoc and signal averaging to improve delineation of retinal layers. In addition, innovative methods for image display, such as C-mode sectioning, provide a unique viewing perspective and may improve interpretation of OCT images of pathologic structures. While all of these methods are being developed, most remain in an immature state. This review describes the current status of 3D-OCT scanning and interpretation, and discusses the need for standardization of clinical protocols as well as the potential benefits of 3D-OCT scanning that could come when software methods for fully exploiting these rich data sets are available clinically. The implications of new image analysis approaches include improved reproducibility of measurements garnered from 3D-OCT, which may then help improve disease discrimination and progression detection. In addition, 3D-OCT offers the potential for preoperative surgical planning and intraoperative surgical guidance. PMID:20542136

A Review of Adaptive Optics Optical Coherence Tomography: Technical Advances, Scientific Applications, and the Future.

PubMed

Jonnal, Ravi S; Kocaoglu, Omer P; Zawadzki, Robert J; Liu, Zhuolin; Miller, Donald T; Werner, John S

2016-07-01

Optical coherence tomography (OCT) has enabled "virtual biopsy" of the living human retina, revolutionizing both basic retina research and clinical practice over the past 25 years. For most of those years, in parallel, adaptive optics (AO) has been used to improve the transverse resolution of ophthalmoscopes to foster in vivo study of the retina at the microscopic level. Here, we review work done over the last 15 years to combine the microscopic transverse resolution of AO with the microscopic axial resolution of OCT, building AO-OCT systems with the highest three-dimensional resolution of any existing retinal imaging modality. We surveyed the literature to identify the most influential antecedent work, important milestones in the development of AO-OCT technology, its applications that have yielded new knowledge, research areas into which it may productively expand, and nascent applications that have the potential to grow. Initial efforts focused on demonstrating three-dimensional resolution. Since then, many improvements have been made in resolution and speed, as well as other enhancements of acquisition and postprocessing techniques. Progress on these fronts has produced numerous discoveries about the anatomy, function, and optical properties of the retina. Adaptive optics OCT continues to evolve technically and to contribute to our basic and clinical knowledge of the retina. Due to its capacity to reveal cellular and microscopic detail invisible to clinical OCT systems, it is an ideal companion to those instruments and has the demonstrable potential to produce images that can guide the interpretation of clinical findings.

Polarization sensitive optical coherence tomography – a review [Invited

PubMed Central

de Boer, Johannes F.; Hitzenberger, Christoph K.; Yasuno, Yoshiaki

2017-01-01

Optical coherence tomography (OCT) is now a well-established modality for high-resolution cross-sectional and three-dimensional imaging of transparent and translucent samples and tissues. Conventional, intensity based OCT, however, does not provide a tissue-specific contrast, causing an ambiguity with image interpretation in several cases. Polarization sensitive (PS) OCT draws advantage from the fact that several materials and tissues can change the light’s polarization state, adding an additional contrast channel and providing quantitative information. In this paper, we review basic and advanced methods of PS-OCT and demonstrate its use in selected biomedical applications. PMID:28663869

Three-dimensional display technologies

PubMed Central

Geng, Jason

2014-01-01

The physical world around us is three-dimensional (3D), yet traditional display devices can show only two-dimensional (2D) flat images that lack depth (i.e., the third dimension) information. This fundamental restriction greatly limits our ability to perceive and to understand the complexity of real-world objects. Nearly 50% of the capability of the human brain is devoted to processing visual information [Human Anatomy & Physiology (Pearson, 2012)]. Flat images and 2D displays do not harness the brain’s power effectively. With rapid advances in the electronics, optics, laser, and photonics fields, true 3D display technologies are making their way into the marketplace. 3D movies, 3D TV, 3D mobile devices, and 3D games have increasingly demanded true 3D display with no eyeglasses (autostereoscopic). Therefore, it would be very beneficial to readers of this journal to have a systematic review of state-of-the-art 3D display technologies. PMID:25530827

Coding Strategies and Implementations of Compressive Sensing

NASA Astrophysics Data System (ADS)

Tsai, Tsung-Han

This dissertation studies the coding strategies of computational imaging to overcome the limitation of conventional sensing techniques. The information capacity of conventional sensing is limited by the physical properties of optics, such as aperture size, detector pixels, quantum efficiency, and sampling rate. These parameters determine the spatial, depth, spectral, temporal, and polarization sensitivity of each imager. To increase sensitivity in any dimension can significantly compromise the others. This research implements various coding strategies subject to optical multidimensional imaging and acoustic sensing in order to extend their sensing abilities. The proposed coding strategies combine hardware modification and signal processing to exploiting bandwidth and sensitivity from conventional sensors. We discuss the hardware architecture, compression strategies, sensing process modeling, and reconstruction algorithm of each sensing system. Optical multidimensional imaging measures three or more dimensional information of the optical signal. Traditional multidimensional imagers acquire extra dimensional information at the cost of degrading temporal or spatial resolution. Compressive multidimensional imaging multiplexes the transverse spatial, spectral, temporal, and polarization information on a two-dimensional (2D) detector. The corresponding spectral, temporal and polarization coding strategies adapt optics, electronic devices, and designed modulation techniques for multiplex measurement. This computational imaging technique provides multispectral, temporal super-resolution, and polarization imaging abilities with minimal loss in spatial resolution and noise level while maintaining or gaining higher temporal resolution. The experimental results prove that the appropriate coding strategies may improve hundreds times more sensing capacity. Human auditory system has the astonishing ability in localizing, tracking, and filtering the selected sound sources or information from a noisy environment. Using engineering efforts to accomplish the same task usually requires multiple detectors, advanced computational algorithms, or artificial intelligence systems. Compressive acoustic sensing incorporates acoustic metamaterials in compressive sensing theory to emulate the abilities of sound localization and selective attention. This research investigates and optimizes the sensing capacity and the spatial sensitivity of the acoustic sensor. The well-modeled acoustic sensor allows localizing multiple speakers in both stationary and dynamic auditory scene; and distinguishing mixed conversations from independent sources with high audio recognition rate.

Origin of coloration in beetle scales: An optical and structural investigation

NASA Astrophysics Data System (ADS)

Nagi, Ramneet Kaur

In this thesis the origin of angle-independent yellowish-green coloration of the exoskeleton of a beetle was studied. The beetle chosen was a weevil with the Latin name Eupholus chevrolati. The origin of this weevil's coloration was investigated by optical and structural characterization techniques, including optical microscopy, scanning electron microscopy imaging and focused ion beam milling, combined with three-dimensional modeling and photonic band structure calculations. Furthermore, using color theory the pixel-like coloring of the weevil's exoskeleton was investigated and an interesting additive color mixing scheme was discovered. For optical studies, a microreflectance microscopy/spectroscopy set-up was optimized. This set-up allowed not only for imaging of individual colored exoskeleton domains with sizes ˜2-10 μm, but also for obtaining reflection spectra of these micrometer-sized domains. Spectra were analyzed in terms of reflection intensity and wavelength position and shape of the reflection features. To find the origin of these colored exoskeleton spots, a combination of focused ion beam milling and scanning electron microscopy imaging was employed. A three-dimensional photonic crystal in the form of a face-centered cubic lattice of ABC-stacked air cylinders in a biopolymeric cuticle matrix was discovered. Our photonic band structure calculations revealed the existence of different sets of stop-gaps for the lattice constant of 360, 380 and 400 nm in the main lattice directions, Gamma-L, Gamma-X, Gamma-U, Gamma-W and Gamma-K. In addition, scanning electron microscopy images were compared to the specific directional-cuts through the constructed face-centered cubic lattice-based model and the optical micrographs of individual domains to determine the photonic structure corresponding to the different lattice directions. The three-dimensional model revealed stop-gaps in the Gamma-L, Gamma-W and Gamma-K directions. Finally, the coloration of the weevil as perceived by an unaided human eye was represented (mathematically) on the xy-chromaticity diagram, based on XYZ color space developed by CIE (Commission Internationale de l'Eclairage), using the micro-reflectance spectroscopy measurements. The results confirmed the additive mixing of various colors produced by differently oriented photonic crystal domains present in the weevil's exoskeleton scales, as a reason for the angle-independent dull yellowish-green coloration of the weevil E. chevrolati.

Dynamical measurements of motion behavior of free fluorescent sphere using the wide field temporal focusing microscopy with astigmatism method (Conference Presentation)

NASA Astrophysics Data System (ADS)

Lien, Chi-Hsiang; Lin, Chun-Yu; Chen, Shean-Jen; Chien, Fan-Ching

2017-02-01

A three-dimensional (3D) single fluorescent particle tracking strategy based on temporal focusing multiphoton excitation microscopy (TFMPEM) combined with astigmatism imaging is proposed for delivering nanoscale-level axial information that reveals 3D trajectories of single fluorospheres in the axially-resolved multiphoton excitation volume without z-axis scanning. It provides the dynamical ability by measuring the diffusion coefficient of fluorospheres in glycerol solutions with a position standard deviation of 14 nm and 21 nm in the lateral and axial direction and a frame rate of 100 Hz. Moreover, the optical trapping force based on the TFMPEM is minimized to avoid the interference in the tracing measurements compared to that in the spatial focusing MPE approaches. Therefore, we presented a three dimensional single particle tracking strategy to overcome the limitation of the time resolution of the multiphoton imaging using fast frame rate of TFMPEM, and provide three dimensional locations of multiple particles using an astigmatism method.

Analysis and design of coaxial three-mirror anastigmat with long effective focal length and full two-dimensional field

NASA Astrophysics Data System (ADS)

Lin, Han; Baoqi, Mao; Wen, Sun; Weimin, Shen

2016-10-01

There is a race to develop spaceborne high-resolution video cameras since Skybox's success. For low manufacture cost and adaption to micro and small satellites, it is urgent to design and develop compact long focal length optical system with not only small volume, light weight and easy implementation, and also two dimensional field. Our focus is on the Coaxial Three-Mirror Anastigmat (CTMA) with intermediate real image for its no need outer hood and compactness and for its easy alignment, low-order aspheric surface and low cost. The means to deflect its image space beam for accessibility of focal plane array detector and to eliminate its inherent secondary obscuration from its primary mirror central hole and deflection flat mirror is discussed. The conditions to satisfy the above-mentioned requirements are presented with our derived relationship among its optical and structural parameters based on Gaussian optics and geometry. One flat mirror near its exit pupil can be used to deflect its image plane from its axis. And its total length can be decreased with other some flat mirrors. Method for determination of its initial structure with the derived formulae is described through one design example. Furthermore, optimized CTMA without secondary obscuration and with effective focal length (EFFL) of 10m is reported. Its full field, F-number and total length are respectively 1.1°×1°, F/14.3, and one eighth of its EFFL. And its imaging quality is near diffraction limit.

Three-photon imaging of ovarian cancer

NASA Astrophysics Data System (ADS)

Barton, Jennifer K.; Amirsolaimani, Babak; Rice, Photini; Hatch, Kenneth; Kieu, Khanh

2016-02-01

Optical imaging methods have the potential to detect ovarian cancer at an early, curable stage. Optical imaging has the disadvantage that high resolution techniques require access to the tissue of interest, but miniature endoscopes that traverse the natural orifice of the reproductive tract, or access the ovaries and fallopian tubes through a small incision in the vagina wall, can provide a minimally-invasive solution. We have imaged both rodent and human ovaries and fallopian tubes with a variety of endoscope-compatible modalities. The recent development of fiber-coupled femtosecond lasers will enable endoscopic multiphoton microscopy (MPM). We demonstrated two- and three-photon excited fluorescence (2PEF, 3PEF), and second- and third-harmonic generation microscopy (SHG, THG) in human ovarian and fallopian tube tissue. A study was undertaken to understand the mechanisms of contrast in these images. Six patients (normal, cystadenoma, and ovarian adenocarcinoma) provided ovarian and fallopian tube biopsies. The tissue was imaged with three-dimensional optical coherence tomography, multiphoton microscopy, and frozen for histological sectioning. Tissue sections were stained with hematoxylin and eosin, Masson's trichrome, and Sudan black. Approximately 1 μm resolution images were obtained with an excitation source at 1550 nm. 2PEF signal was absent. SHG signal was mainly from collagen. 3PEF and THG signal came from a variety of sources, including a strong signal from fatty connective tissue and red blood cells. Adenocarcinoma was characterized by loss of SHG signal, whereas cystic abnormalities showed strong SHG. There was limited overlap of two- and three- photon signals, suggesting that three-photon imaging can provide additional information for early diagnosis of ovarian cancer.

Phase retrieval and 3D imaging in gold nanoparticles based fluorescence microscopy (Conference Presentation)

NASA Astrophysics Data System (ADS)

Ilovitsh, Tali; Ilovitsh, Asaf; Weiss, Aryeh M.; Meir, Rinat; Zalevsky, Zeev

2017-02-01

Optical sectioning microscopy can provide highly detailed three dimensional (3D) images of biological samples. However, it requires acquisition of many images per volume, and is therefore time consuming, and may not be suitable for live cell 3D imaging. We propose the use of the modified Gerchberg-Saxton phase retrieval algorithm to enable full 3D imaging of gold nanoparticles tagged sample using only two images. The reconstructed field is free space propagated to all other focus planes using post processing, and the 2D z-stack is merged to create a 3D image of the sample with high fidelity. Because we propose to apply the phase retrieving on nano particles, the regular ambiguities typical to the Gerchberg-Saxton algorithm, are eliminated. The proposed concept is then further enhanced also for tracking of single fluorescent particles within a three dimensional (3D) cellular environment based on image processing algorithms that can significantly increases localization accuracy of the 3D point spread function in respect to regular Gaussian fitting. All proposed concepts are validated both on simulated data as well as experimentally.

Non Contacting Evaluation of Strains and Cracking Using Optical and Infrared Imaging Techniques

DTIC Science & Technology

1988-08-22

Compatible Zenith Z-386 microcomputer with plotter II. 3-D Motion Measurinq System 1. Complete OPTOTRAK three dimensional digitizing system. System includes...acquisition unit - 16 single ended analog input channels 3. Data Analysis Package software (KINEPLOT) 4. Extra OPTOTRAK Camera (max 224 per system

Three-dimensional imaging and remote sensing imaging; Proceedings of the Meeting, Los Angeles, CA, Jan. 14, 15, 1988

NASA Astrophysics Data System (ADS)

Robbins, Woodrow E.

1988-01-01

The present conference discusses topics in novel technologies and techniques of three-dimensional imaging, human factors-related issues in three-dimensional display system design, three-dimensional imaging applications, and image processing for remote sensing. Attention is given to a 19-inch parallactiscope, a chromostereoscopic CRT-based display, the 'SpaceGraph' true three-dimensional peripheral, advantages of three-dimensional displays, holographic stereograms generated with a liquid crystal spatial light modulator, algorithms and display techniques for four-dimensional Cartesian graphics, an image processing system for automatic retina diagnosis, the automatic frequency control of a pulsed CO2 laser, and a three-dimensional display of magnetic resonance imaging of the spine.

Refocusing-range and image-quality enhanced optical reconstruction of 3-D objects from integral images using a principal periodic δ-function array

NASA Astrophysics Data System (ADS)

Ai, Lingyu; Kim, Eun-Soo

2018-03-01

We propose a method for refocusing-range and image-quality enhanced optical reconstruction of three-dimensional (3-D) objects from integral images only by using a 3 × 3 periodic δ-function array (PDFA), which is called a principal PDFA (P-PDFA). By directly convolving the elemental image array (EIA) captured from 3-D objects with the P-PDFAs whose spatial periods correspond to each object's depth, a set of spatially-filtered EIAs (SF-EIAs) are extracted, and from which 3-D objects can be reconstructed to be refocused on their real depth. convolutional operations are performed directly on each of the minimum 3 × 3 EIs of the picked-up EIA, the capturing and refocused-depth ranges of 3-D objects can be greatly enhanced, as well as 3-D objects much improved in image quality can be reconstructed without any preprocessing operations. Through ray-optical analysis and optical experiments with actual 3-D objects, the feasibility of the proposed method has been confirmed.

Back-to-back optical coherence tomography-ultrasound probe for co-registered three-dimensional intravascular imaging with real-time display

NASA Astrophysics Data System (ADS)

Li, Jiawen; Ma, Teng; Jing, Joseph; Zhang, Jun; Patel, Pranav M.; Shung, K. Kirk; Zhou, Qifa; Chen, Zhongping

2014-03-01

We have developed a novel integrated optical coherence tomography (OCT)-intravascular ultrasound (IVUS) probe, with a 1.5 mm-long rigid-part and 0.9 mm outer diameter, for real-time intracoronary imaging of atherosclerotic plaques and guiding interventional procedures. By placing the OCT ball lens and IVUS 45MHz single element transducer back-to-back at the same axial position, this probe can provide automatically co-registered, co-axial OCT-IVUS imaging. To demonstrate its capability, 3D OCT-IVUS imaging of a pig's coronary artery in real-time displayed in polar coordinates, as well as images of two major types of advanced plaques in human cadaver coronary segments, was obtained using this probe and our upgraded system. Histology validation is also presented.

Coherence-domain imaging with harmonic holography

NASA Astrophysics Data System (ADS)

Pu, Ye; Psaltis, Demetri

2017-08-01

Observing the fast dynamics of specific molecules or targets in three-dimensional (3D) space and time inside a crowded and complex environment, such as living cells or tissues, remain one of the grand open challenges in modern science. Harmonic holography tackle this challenge by combining the 3D imaging capability of holography with the ultrafast, coherent optical contrast offered by second-harmonic radiating imaging probes (SHRIMPs). Similar to fluorescence, the second-harmonic signal emitted from SHRIMPs provides a color contrast against the uninterested background scattering, which can be efficiently suppressed by an optical filter. We review the latest developments in SHRIMPs and harmonic holography and discuss their further applications in fluidics and biofluidics.

Colposcopic imaging using visible-light optical coherence tomography.

PubMed

Duan, Lian; McRaven, Michael D; Liu, Wenzhong; Shu, Xiao; Hu, Jianmin; Sun, Cheng; Veazey, Ronald S; Hope, Thomas J; Zhang, Hao F

2017-05-01

High-resolution colposcopic optical coherence tomography (OCT) provides key anatomical measures, such as thickness and minor traumatic injury of vaginal epithelium, of the female reproductive tract noninvasively. This information can be helpful in both fundamental investigations in animal models and disease screenings in humans. We present a fiber-based visible-light OCT and two probe designs for colposcopic application. One probe conducts circular scanning using a DC motor, and the other probe is capable of three-dimensional imaging over a 4.6 × 4.6 - mm 2 area using a pair of galvo scanners. Using this colposcopic vis-OCT with both probes, we acquired high-resolution images from whole isolated macaque vaginal samples and identified biopsy lesions.

Colposcopic imaging using visible-light optical coherence tomography

NASA Astrophysics Data System (ADS)

Duan, Lian; McRaven, Michael D.; Liu, Wenzhong; Shu, Xiao; Hu, Jianmin; Sun, Cheng; Veazey, Ronald S.; Hope, Thomas J.; Zhang, Hao F.

2017-05-01

High-resolution colposcopic optical coherence tomography (OCT) provides key anatomical measures, such as thickness and minor traumatic injury of vaginal epithelium, of the female reproductive tract noninvasively. This information can be helpful in both fundamental investigations in animal models and disease screenings in humans. We present a fiber-based visible-light OCT and two probe designs for colposcopic application. One probe conducts circular scanning using a DC motor, and the other probe is capable of three-dimensional imaging over a 4.6×4.6-mm2 area using a pair of galvo scanners. Using this colposcopic vis-OCT with both probes, we acquired high-resolution images from whole isolated macaque vaginal samples and identified biopsy lesions.

Blind restoration method of three-dimensional microscope image based on RL algorithm

NASA Astrophysics Data System (ADS)

Yao, Jin-li; Tian, Si; Wang, Xiang-rong; Wang, Jing-li

2013-08-01

Thin specimens of biological tissue appear three dimensional transparent under a microscope. The optic slice images can be captured by moving the focal planes at the different locations of the specimen. The captured image has low resolution due to the influence of the out-of-focus information comes from the planes adjacent to the local plane. Using traditional methods can remove the blur in the images at a certain degree, but it needs to know the point spread function (PSF) of the imaging system accurately. The accuracy degree of PSF influences the restoration result greatly. In fact, it is difficult to obtain the accurate PSF of the imaging system. In order to restore the original appearance of the specimen under the conditions of the imaging system parameters are unknown or there is noise and spherical aberration in the system, a blind restoration methods of three-dimensional microscope based on the R-L algorithm is proposed in this paper. On the basis of the exhaustive study of the two-dimension R-L algorithm, according to the theory of the microscopy imaging and the wavelet transform denoising pretreatment, we expand the R-L algorithm to three-dimension space. It is a nonlinear restoration method with the maximum entropy constraint. The method doesn't need to know the PSF of the microscopy imaging system precisely to recover the blur image. The image and PSF converge to the optimum solutions by many alterative iterations and corrections. The matlab simulation and experiments results show that the expansion algorithm is better in visual indicators, peak signal to noise ratio and improved signal to noise ratio when compared with the PML algorithm, and the proposed algorithm can suppress noise, restore more details of target, increase image resolution.

Super-resolution photon-efficient imaging by nanometric double-helix point spread function localization of emitters (SPINDLE)

PubMed Central

Grover, Ginni; DeLuca, Keith; Quirin, Sean; DeLuca, Jennifer; Piestun, Rafael

2012-01-01

Super-resolution imaging with photo-activatable or photo-switchable probes is a promising tool in biological applications to reveal previously unresolved intra-cellular details with visible light. This field benefits from developments in the areas of molecular probes, optical systems, and computational post-processing of the data. The joint design of optics and reconstruction processes using double-helix point spread functions (DH-PSF) provides high resolution three-dimensional (3D) imaging over a long depth-of-field. We demonstrate for the first time a method integrating a Fisher information efficient DH-PSF design, a surface relief optical phase mask, and an optimal 3D localization estimator. 3D super-resolution imaging using photo-switchable dyes reveals the 3D microtubule network in mammalian cells with localization precision approaching the information theoretical limit over a depth of 1.2 µm. PMID:23187521

Optical mesoscopy without the scatter: broadband multispectral optoacoustic mesoscopy

PubMed Central

Chekkoury, Andrei; Gateau, Jérôme; Driessen, Wouter; Symvoulidis, Panagiotis; Bézière, Nicolas; Feuchtinger, Annette; Walch, Axel; Ntziachristos, Vasilis

2015-01-01

Optical mesoscopy extends the capabilities of biological visualization beyond the limited penetration depth achieved by microscopy. However, imaging of opaque organisms or tissues larger than a few hundred micrometers requires invasive tissue sectioning or chemical treatment of the specimen for clearing photon scattering, an invasive process that is regardless limited with depth. We developed previously unreported broadband optoacoustic mesoscopy as a tomographic modality to enable imaging of optical contrast through several millimeters of tissue, without the need for chemical treatment of tissues. We show that the unique combination of three-dimensional projections over a broad 500 kHz–40 MHz frequency range combined with multi-wavelength illumination is necessary to render broadband multispectral optoacoustic mesoscopy (2B-MSOM) superior to previous optical or optoacoustic mesoscopy implementations. PMID:26417486

Generation of light-sheet at the end of multimode fibre (Conference Presentation)

NASA Astrophysics Data System (ADS)

Plöschner, Martin; Kollárová, Véra; Dostál, Zbyněk.; Nylk, Jonathan; Barton-Owen, Thomas; Ferrier, David E. K.; Chmelik, Radim; Dholakia, Kishan; Cizmár, TomáÅ.¡

2017-02-01

Light-sheet fluorescence microscopy is quickly becoming one of the cornerstone imaging techniques in biology as it provides rapid, three-dimensional sectioning of specimens at minimal levels of phototoxicity. It is very appealing to bring this unique combination of imaging properties into an endoscopic setting and be able to perform optical sectioning deep in tissues. Current endoscopic approaches for delivery of light-sheet illumination are based on single-mode optical fibre terminated by cylindrical gradient index lens. Such configuration generates a light-sheet plane that is axially fixed and a mechanical movement of either the sample or the endoscope is required to acquire three-dimensional information about the sample. Furthermore, the axial resolution of this technique is limited to 5um. The delivery of the light-sheet through the multimode fibre provides better axial resolution limited only by its numerical aperture, the light-sheet is scanned holographically without any mechanical movement, and multiple advanced light-sheet imaging modalities, such as Bessel and structured illumination Bessel beam, are intrinsically supported by the system due to the cylindrical symmetry of the fibre. We discuss the holographic techniques for generation of multiple light-sheet types and demonstrate the imaging on a sample of fluorescent beads fixed in agarose gel, as well as on a biological sample of Spirobranchus Lamarcki.

Three-dimensional imaging of the developing mouse female reproductive organs with optical coherence tomography

NASA Astrophysics Data System (ADS)

Burton, Jason C.; Wang, Shang; Behringer, Richard R.; Larina, Irina V.

2016-03-01

Infertility is a known major health concern and is estimated to impact ~15% of couples in the U.S. The majority of failed pregnancies occur before or during implantation of the fertilized embryo into the uterus. Understanding the mechanisms regulating development by studying mouse reproductive organs could significantly contribute to an improved understanding of normal development of reproductive organs and developmental causes of infertility in humans. Towards this goal, we report a three-dimensional (3D) imaging study of the developing mouse reproductive organs (ovary, oviduct, and uterus) using optical coherence tomography (OCT). In our study, OCT was used for 3D imaging of reproductive organs without exogenous contrast agents and provides micro-scale spatial resolution. Experiments were conducted in vitro on mouse reproductive organs ranging from the embryonic day 14.5 to adult stages. Structural features of the ovary, oviduct, and uterus are presented. Additionally, a comparison with traditional histological analysis is illustrated. These results provide a basis for a wide range of infertility studies in mouse models. Through integration with traditional genetic and molecular biology approaches, this imaging method can improve understanding of ovary, oviduct, and uterus development and function, serving to further contribute to our understanding of fertility and infertility.

A study of the comparative anatomy of the brain of domestic ruminants using magnetic resonance imaging.

PubMed

Schmidt, M J; Langen, N; Klumpp, S; Nasirimanesh, F; Shirvanchi, P; Ondreka, N; Kramer, M

2012-01-01

Although magnetic resonance imaging has been used to examine the brain of domestic ruminants, detailed information relating the precise anatomical features in these species is lacking. In this study the brain structures of calves (Bos taurus domesticus), sheep (Ovis aries), goats (Capra hircus) and a mesaticephalic dog (Canis lupis familiaris) were examined using T2-weighed Turbo Spin Echo sequences; three-dimensional models based on high-resolution gradient echo scans were used to identify brain sulci and gyri in two-dimensional images. The ruminant brains examined were similar in structure and organisation to those of other mammals but particular features included the deep depression of the insula and the pronounced gyri of the cortices, the dominant position of the visual (optic nerve, optic chiasm and rostral colliculus) and olfactory (olfactory bulb, olfactory tracts and piriform lobe) systems, and the relatively large size of the diencephalon. Copyright © 2010 Elsevier Ltd. All rights reserved.

Optical Coherence Tomography for Brain Imaging and Developmental Biology

PubMed Central

Men, Jing; Huang, Yongyang; Solanki, Jitendra; Zeng, Xianxu; Alex, Aneesh; Jerwick, Jason; Zhang, Zhan; Tanzi, Rudolph E.; Li, Airong; Zhou, Chao

2016-01-01

Optical coherence tomography (OCT) is a promising research tool for brain imaging and developmental biology. Serving as a three-dimensional optical biopsy technique, OCT provides volumetric reconstruction of brain tissues and embryonic structures with micrometer resolution and video rate imaging speed. Functional OCT enables label-free monitoring of hemodynamic and metabolic changes in the brain in vitro and in vivo in animal models. Due to its non-invasiveness nature, OCT enables longitudinal imaging of developing specimens in vivo without potential damage from surgical operation, tissue fixation and processing, and staining with exogenous contrast agents. In this paper, various OCT applications in brain imaging and developmental biology are reviewed, with a particular focus on imaging heart development. In addition, we report findings on the effects of a circadian gene (Clock) and high-fat-diet on heart development in Drosophila melanogaster. These findings contribute to our understanding of the fundamental mechanisms connecting circadian genes and obesity to heart development and cardiac diseases. PMID:27721647

Optic cup segmentation: type-II fuzzy thresholding approach and blood vessel extraction

PubMed Central

Almazroa, Ahmed; Alodhayb, Sami; Raahemifar, Kaamran; Lakshminarayanan, Vasudevan

2017-01-01

We introduce here a new technique for segmenting optic cup using two-dimensional fundus images. Cup segmentation is the most challenging part of image processing of the optic nerve head due to the complexity of its structure. Using the blood vessels to segment the cup is important. Here, we report on blood vessel extraction using first a top-hat transform and Otsu’s segmentation function to detect the curves in the blood vessels (kinks) which indicate the cup boundary. This was followed by an interval type-II fuzzy entropy procedure. Finally, the Hough transform was applied to approximate the cup boundary. The algorithm was evaluated on 550 fundus images from a large dataset, which contained three different sets of images, where the cup was manually marked by six ophthalmologists. On one side, the accuracy of the algorithm was tested on the three image sets independently. The final cup detection accuracy in terms of area and centroid was calculated to be 78.2% of 441 images. Finally, we compared the algorithm performance with manual markings done by the six ophthalmologists. The agreement was determined between the ophthalmologists as well as the algorithm. The best agreement was between ophthalmologists one, two and five in 398 of 550 images, while the algorithm agreed with them in 356 images. PMID:28515636

Optic cup segmentation: type-II fuzzy thresholding approach and blood vessel extraction.

PubMed

Almazroa, Ahmed; Alodhayb, Sami; Raahemifar, Kaamran; Lakshminarayanan, Vasudevan

2017-01-01

We introduce here a new technique for segmenting optic cup using two-dimensional fundus images. Cup segmentation is the most challenging part of image processing of the optic nerve head due to the complexity of its structure. Using the blood vessels to segment the cup is important. Here, we report on blood vessel extraction using first a top-hat transform and Otsu's segmentation function to detect the curves in the blood vessels (kinks) which indicate the cup boundary. This was followed by an interval type-II fuzzy entropy procedure. Finally, the Hough transform was applied to approximate the cup boundary. The algorithm was evaluated on 550 fundus images from a large dataset, which contained three different sets of images, where the cup was manually marked by six ophthalmologists. On one side, the accuracy of the algorithm was tested on the three image sets independently. The final cup detection accuracy in terms of area and centroid was calculated to be 78.2% of 441 images. Finally, we compared the algorithm performance with manual markings done by the six ophthalmologists. The agreement was determined between the ophthalmologists as well as the algorithm. The best agreement was between ophthalmologists one, two and five in 398 of 550 images, while the algorithm agreed with them in 356 images.

Miniature endoscopic optical coherence tomography for calculus detection.

PubMed

Kao, Meng-Chun; Lin, Chun-Li; Kung, Che-Yen; Huang, Yi-Fung; Kuo, Wen-Chuan

2015-08-20

The effective treatment of periodontitis involves the detection and removal of subgingival dental calculus. However, subgingival calculus is more difficult to detect than supragingival calculus because it is firmly attached to root surfaces within periodontal pockets. To achieve a smooth root surface, clinicians often remove excessive amounts of root structure because of decreased visibility. In addition, enamel pearl, a rare type of ectopic enamel formation on the root surface, can easily be confused with dental calculus in the subgingival environment. In this study, we developed a fiber-probe swept-source optical coherence tomography (SSOCT) technique and combined it with the quantitative measurement of an optical parameter [standard deviation (SD) of the optical coherence tomography (OCT) intensity] to differentiate subgingival calculus from sound enamel, including enamel pearl. Two-dimensional circumferential images were constructed by rotating the miniprobe (0.9 mm diameter) while acquiring image lines, and the adjacent lines in each rotation were stacked to generate a three-dimensional volume. In OCT images, compared to sound enamel and enamel pearls, dental calculus showed significant differences (P<0.001) in SD values. Finally, the receiver operating characteristic curve had a high capacity (area under the curve=0.934) for discriminating between healthy regions (including enamel pearl) and dental calculus.

Measurement of three-dimensional velocity profiles using forward scattering particle image velocimetry (FSPIV) and neural net pattern recognition

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ovryn, B.; Wright, T.; Khaydarov, J.D.

1995-12-31

The authors employ Forward Scattering Particle Image Velocimetry (FSPIV) to measure all three components of the velocity of a buoyant polystyrene particle in oil. Unlike conventional particle image velocimetry (PIV) techniques, FSPIV employs coherent or partially coherent back illumination and collects the forward scattered wavefront; additionally, the field-of-view is microscopic. Using FSPIV, it is possible to easily identify the particle`s centroid and to simultaneously obtain the fluid velocity in different planes perpendicular to the viewing direction without changing the collection or imaging optics. The authors have trained a neural network to identify the scattering pattern as function of displacement alongmore » the optical axis (axial defocus) and determine the transverse velocity by tracking the centroid as function of time. They present preliminary results from Mie theory calculations which include the effect of the imaging system. To their knowledge, this is the first work of this kind; preliminary results are encouraging.« less

Freehand three-dimensional ultrasound imaging of carotid artery using motion tracking technology.

PubMed

Chung, Shao-Wen; Shih, Cho-Chiang; Huang, Chih-Chung

2017-02-01

Ultrasound imaging has been extensively used for determining the severity of carotid atherosclerotic stenosis. In particular, the morphological characterization of carotid plaques can be performed for risk stratification of patients. However, using 2D ultrasound imaging for detecting morphological changes in plaques has several limitations. Due to the scan was performed on a single longitudinal cross-section, the selected 2D image is difficult to represent the entire morphology and volume of plaque and vessel lumen. In addition, the precise positions of 2D ultrasound images highly depend on the radiologists' experience, it makes the serial long-term exams of anti-atherosclerotic therapies are difficult to relocate the same corresponding planes by using 2D B-mode images. This has led to the recent development of three-dimensional (3D) ultrasound imaging, which offers improved visualization and quantification of complex morphologies of carotid plaques. In the present study, a freehand 3D ultrasound imaging technique based on optical motion tracking technology is proposed. Unlike other optical tracking systems, the marker is a small rigid body that is attached to the ultrasound probe and is tracked by eight high-performance digital cameras. The probe positions in 3D space coordinates are then calibrated at spatial and temporal resolutions of 10μm and 0.01s, respectively. The image segmentation procedure involves Otsu's and the active contour model algorithms and accurately detects the contours of the carotid arteries. The proposed imaging technique was verified using normal artery and atherosclerotic stenosis phantoms. Human experiments involving freehand scanning of the carotid artery of a volunteer were also performed. The results indicated that compared with manual segmentation, the lowest percentage errors of the proposed segmentation procedure were 7.8% and 9.1% for the external and internal carotid arteries, respectively. Finally, the effect of handshaking was calibrated using the optical tracking system for reconstructing a 3D image. Copyright © 2016 Elsevier B.V. All rights reserved.

Learning from Monet: A Fundamentally New Approach to Image Analysis

NASA Astrophysics Data System (ADS)

Falco, Charles M.

2009-03-01

The hands and minds of artists are intimately involved in the creative process, intrinsically making paintings complex images to analyze. In spite of this difficulty, several years ago the painter David Hockney and I identified optical evidence within a number of paintings that demonstrated artists as early as Jan van Eyck (c1425) used optical projections as aids for producing portions of their images. In the course of making those discoveries, Hockney and I developed new insights that are now being applied in a fundamentally new approach to image analysis. Very recent results from this new approach include identifying from Impressionist paintings by Monet, Pissarro, Renoir and others the precise locations the artists stood when making a number of their paintings. The specific deviations we find when accurately comparing these examples with photographs taken from the same locations provide us with key insights into what the artists' visual skills informed them were the ways to represent these two-dimensional images of three-dimensional scenes to viewers. As will be discussed, these results also have implications for improving the representation of certain scientific data. Acknowledgment: I am grateful to David Hockney for the many invaluable insights into imaging gained from him in our collaboration.

HCIT Contrast Performance Sensitivity Studies: Simulation Versus Experiment

NASA Technical Reports Server (NTRS)

Sidick, Erkin; Shaklan, Stuart; Krist, John; Cady, Eric J.; Kern, Brian; Balasubramanian, Kunjithapatham

2013-01-01

Using NASA's High Contrast Imaging Testbed (HCIT) at the Jet Propulsion Laboratory, we have experimentally investigated the sensitivity of dark hole contrast in a Lyot coronagraph for the following factors: 1) Lateral and longitudinal translation of an occulting mask; 2) An opaque spot on the occulting mask; 3) Sizes of the controlled dark hole area. Also, we compared the measured results with simulations obtained using both MACOS (Modeling and Analysis for Controlled Optical Systems) and PROPER optical analysis programs with full three-dimensional near-field diffraction analysis to model HCIT's optical train and coronagraph.

Tomographic diffractive microscopy with agile illuminations for imaging targets in a noisy background.

PubMed

Zhang, T; Godavarthi, C; Chaumet, P C; Maire, G; Giovannini, H; Talneau, A; Prada, C; Sentenac, A; Belkebir, K

2015-02-15

Tomographic diffractive microscopy is a marker-free optical digital imaging technique in which three-dimensional samples are reconstructed from a set of holograms recorded under different angles of incidence. We show experimentally that, by processing the holograms with singular value decomposition, it is possible to image objects in a noisy background that are invisible with classical wide-field microscopy and conventional tomographic reconstruction procedure. The targets can be further characterized with a selective quantitative inversion.

Fluorescence Molecular Tomography: Principles and Potential for Pharmaceutical Research

PubMed Central

Stuker, Florian; Ripoll, Jorge; Rudin, Markus

2011-01-01

Fluorescence microscopic imaging is widely used in biomedical research to study molecular and cellular processes in cell culture or tissue samples. This is motivated by the high inherent sensitivity of fluorescence techniques, the spatial resolution that compares favorably with cellular dimensions, the stability of the fluorescent labels used and the sophisticated labeling strategies that have been developed for selectively labeling target molecules. More recently, two and three-dimensional optical imaging methods have also been applied to monitor biological processes in intact biological organisms such as animals or even humans. These whole body optical imaging approaches have to cope with the fact that biological tissue is a highly scattering and absorbing medium. As a consequence, light propagation in tissue is well described by a diffusion approximation and accurate reconstruction of spatial information is demanding. While in vivo optical imaging is a highly sensitive method, the signal is strongly surface weighted, i.e., the signal detected from the same light source will become weaker the deeper it is embedded in tissue, and strongly depends on the optical properties of the surrounding tissue. Derivation of quantitative information, therefore, requires tomographic techniques such as fluorescence molecular tomography (FMT), which maps the three-dimensional distribution of a fluorescent probe or protein concentration. The combination of FMT with a structural imaging method such as X-ray computed tomography (CT) or Magnetic Resonance Imaging (MRI) will allow mapping molecular information on a high definition anatomical reference and enable the use of prior information on tissue's optical properties to enhance both resolution and sensitivity. Today many of the fluorescent assays originally developed for studies in cellular systems have been successfully translated for experimental studies in animals. The opportunity of monitoring molecular processes non-invasively in the intact organism is highly attractive from a diagnostic point of view but even more so for the drug developer, who can use the techniques for proof-of-mechanism and proof-of-efficacy studies. This review shall elucidate the current status and potential of fluorescence tomography including recent advances in multimodality imaging approaches for preclinical and clinical drug development. PMID:24310495

A resolution measure for three-dimensional microscopy

PubMed Central

Chao, Jerry; Ram, Sripad; Abraham, Anish V.; Ward, E. Sally; Ober, Raimund J.

2009-01-01

A three-dimensional (3D) resolution measure for the conventional optical microscope is introduced which overcomes the drawbacks of the classical 3D (axial) resolution limit. Formulated within the context of a parameter estimation problem and based on the Cramer-Rao lower bound, this 3D resolution measure indicates the accuracy with which a given distance between two objects in 3D space can be determined from the acquired image. It predicts that, given enough photons from the objects of interest, arbitrarily small distances of separation can be estimated with prespecified accuracy. Using simulated images of point source pairs, we show that the maximum likelihood estimator is capable of attaining the accuracy predicted by the resolution measure. We also demonstrate how different factors, such as extraneous noise sources and the spatial orientation of the imaged object pair, can affect the accuracy with which a given distance of separation can be determined. PMID:20161040

Design and laboratory calibration of the compact pushbroom hyperspectral imaging system

NASA Astrophysics Data System (ADS)

Zhou, Jiankang; Ji, Yiqun; Chen, Yuheng; Chen, Xinhua; Shen, Weimin

2009-11-01

The designed hyperspectral imaging system is composed of three main parts, that is, optical subsystem, electronic subsystem and capturing subsystem. And a three-dimensional "image cube" can be obtained through push-broom. The fore-optics is commercial-off-the-shelf with high speed and three continuous zoom ratios. Since the dispersive imaging part is based on Offner relay configuration with an aberration-corrected convex grating, high power of light collection and variable view field are obtained. The holographic recording parameters of the convex grating are optimized, and the aberration of the Offner configuration dispersive system is balanced. The electronic system adopts module design, which can minimize size, mass, and power consumption. Frame transfer area-array CCD is chosen as the image sensor and the spectral line can be binned to achieve better SNR and sensitivity without any deterioration in spatial resolution. The capturing system based on the computer can set the capturing parameters, calibrate the spectrometer, process and display spectral imaging data. Laboratory calibrations are prerequisite for using precise spectral data. The spatial and spectral calibration minimize smile and keystone distortion caused by optical system, assembly and so on and fix positions of spatial and spectral line on the frame area-array CCD. Gases excitation lamp is used in smile calibration and the keystone calculation is carried out by different viewing field point source created by a series of narrow slit. The laboratory and field imaging results show that this pushbroom hyperspectral imaging system can acquire high quality spectral images.

Correlation Plenoptic Imaging.

PubMed

D'Angelo, Milena; Pepe, Francesco V; Garuccio, Augusto; Scarcelli, Giuliano

2016-06-03

Plenoptic imaging is a promising optical modality that simultaneously captures the location and the propagation direction of light in order to enable three-dimensional imaging in a single shot. However, in standard plenoptic imaging systems, the maximum spatial and angular resolutions are fundamentally linked; thereby, the maximum achievable depth of field is inversely proportional to the spatial resolution. We propose to take advantage of the second-order correlation properties of light to overcome this fundamental limitation. In this Letter, we demonstrate that the correlation in both momentum and position of chaotic light leads to the enhanced refocusing power of correlation plenoptic imaging with respect to standard plenoptic imaging.

Correlation Plenoptic Imaging

NASA Astrophysics Data System (ADS)

D'Angelo, Milena; Pepe, Francesco V.; Garuccio, Augusto; Scarcelli, Giuliano

2016-06-01

Plenoptic imaging is a promising optical modality that simultaneously captures the location and the propagation direction of light in order to enable three-dimensional imaging in a single shot. However, in standard plenoptic imaging systems, the maximum spatial and angular resolutions are fundamentally linked; thereby, the maximum achievable depth of field is inversely proportional to the spatial resolution. We propose to take advantage of the second-order correlation properties of light to overcome this fundamental limitation. In this Letter, we demonstrate that the correlation in both momentum and position of chaotic light leads to the enhanced refocusing power of correlation plenoptic imaging with respect to standard plenoptic imaging.

Multifocus microscopy with precise color multi-phase diffractive optics applied in functional neuronal imaging.

PubMed

Abrahamsson, Sara; Ilic, Rob; Wisniewski, Jan; Mehl, Brian; Yu, Liya; Chen, Lei; Davanco, Marcelo; Oudjedi, Laura; Fiche, Jean-Bernard; Hajj, Bassam; Jin, Xin; Pulupa, Joan; Cho, Christine; Mir, Mustafa; El Beheiry, Mohamed; Darzacq, Xavier; Nollmann, Marcelo; Dahan, Maxime; Wu, Carl; Lionnet, Timothée; Liddle, J Alexander; Bargmann, Cornelia I

2016-03-01

Multifocus microscopy (MFM) allows high-resolution instantaneous three-dimensional (3D) imaging and has been applied to study biological specimens ranging from single molecules inside cells nuclei to entire embryos. We here describe pattern designs and nanofabrication methods for diffractive optics that optimize the light-efficiency of the central optical component of MFM: the diffractive multifocus grating (MFG). We also implement a "precise color" MFM layout with MFGs tailored to individual fluorophores in separate optical arms. The reported advancements enable faster and brighter volumetric time-lapse imaging of biological samples. In live microscopy applications, photon budget is a critical parameter and light-efficiency must be optimized to obtain the fastest possible frame rate while minimizing photodamage. We provide comprehensive descriptions and code for designing diffractive optical devices, and a detailed methods description for nanofabrication of devices. Theoretical efficiencies of reported designs is ≈90% and we have obtained efficiencies of > 80% in MFGs of our own manufacture. We demonstrate the performance of a multi-phase MFG in 3D functional neuronal imaging in living C. elegans.

Feasibility evaluation of 3D photoacoustic imaging of blood vessel structure using multiple wavelengths with a handheld probe

NASA Astrophysics Data System (ADS)

Uchimoto, Yo; Namita, Takeshi; Kondo, Kengo; Yamakawa, Makoto; Shiina, Tsuyoshi

2018-02-01

Photoacoustic imaging is anticipated for use in portraying blood vessel structures (e.g. neovascularization in inflamed regions). To reduce invasiveness and enhance ease handling, we developed a handheld photoacoustic imaging system using multiple wavelengths. The usefulness of the proposed system was investigated in phantom experiments and in vivo measurements. A silicon tube was embedded into chicken breast meat to simulate the blood vessel. The tube was filled with ovine blood. Then laser light was guided to the phantom surface by an optical fiber bundle close to the linear ultrasound probe. Photoacoustic images were obtained at 750-950 nm wavelengths. Strong photoacoustic signals from the boundary between blood and silicon tube are observed in these images. The shape of photoacoustic spectrum at the boundary resembles that of the HbO2 absorption spectrum at 750-920 nm. In photoacoustic images, similarity between photoacoustic spectrum and HbO2 absorption spectrum was evaluated by calculating the normalized correlation coefficient. Results show high correlation in regions of strong photoacoustic signals in photoacoustic images. These analyses demonstrate the feasibility of portraying blood vessel structures under practical conditions. To evaluate the feasibility of three-dimensional vascular imaging, in vivo experiments were conducted using three wavelengths. A right hand and ultrasound probe were set in degassed water. By scanning a probe, cross-sectional ultrasound and photoacoustic images were obtained at each location. Then, all ultrasound or photoacoustic images were piled up respectively. Then three-dimensional images were constructed. Resultant images portrayed blood vessel-like structures three-dimensionally. Furthermore, to distinguish blood vessels from other tissues (e.g. skin), distinguishing images of them were constructed by comparing photoacoustic signal intensity among three wavelengths. The resultant image portrayed blood vessels as distinguished from surrounding tissues. These results demonstrated the usefulness of the proposed imaging device.

Fast Two-Dimensional Bubble Analysis of Biopolymer Filamentous Networks Pore Size from Confocal Microscopy Thin Data Stacks

PubMed Central

Molteni, Matteo; Magatti, Davide; Cardinali, Barbara; Rocco, Mattia; Ferri, Fabio

2013-01-01

The average pore size ξ0 of filamentous networks assembled from biological macromolecules is one of the most important physical parameters affecting their biological functions. Modern optical methods, such as confocal microscopy, can noninvasively image such networks, but extracting a quantitative estimate of ξ0 is a nontrivial task. We present here a fast and simple method based on a two-dimensional bubble approach, which works by analyzing one by one the (thresholded) images of a series of three-dimensional thin data stacks. No skeletonization or reconstruction of the full geometry of the entire network is required. The method was validated by using many isotropic in silico generated networks of different structures, morphologies, and concentrations. For each type of network, the method provides accurate estimates (a few percent) of the average and the standard deviation of the three-dimensional distribution of the pore sizes, defined as the diameters of the largest spheres that can be fit into the pore zones of the entire gel volume. When applied to the analysis of real confocal microscopy images taken on fibrin gels, the method provides an estimate of ξ0 consistent with results from elastic light scattering data. PMID:23473499

Conceptual design of a coherent optical system of modular imaging collectors (COSMIC). [telescope array deployed by space shuttle in 1990's

NASA Technical Reports Server (NTRS)

Nein, M. E.; Davis, B. G.

1982-01-01

The Coherent Optical System of Modular Imaging Collectors (COSMIC) is the design concept for a phase-coherent optical telescope array that may be placed in earth orbit by the Space Shuttle in the 1990s. The initial system module is a minimum redundancy array whose photon collecting area is three times larger than that of the Space Telescope, and possesses a one-dimensional resoution of better than 0.01 arcsec in the visible range. Thermal structural requirements are assessed. Although the coherent beam combination requirements will be met by an active control system, the COSMIC structural/thermal design must meet more stringent performance criteria than even those of the Space Telescope.

Accurate geometrical optics model for single-lens stereovision system using a prism.

PubMed

Cui, Xiaoyu; Lim, Kah Bin; Guo, Qiyong; Wang, DaoLei

2012-09-01

In this paper, we proposed a new method for analyzing the image formation of a prism. The prism was considered as a single optical system composed of some planes. By analyzing each plane individually and then combining them together, we derived a transformation matrix which can express the relationship between an object point and its image by the refraction of a prism. We also explained how to use this matrix for epipolar geometry and three-dimensional point reconstruction. Our method is based on optical geometry and could be used in a multiocular prism. Experimentation results are presented to prove the accuracy of our method is better than former researchers' and is comparable with that of the multicamera stereovision system.

Tomographic PIV behind a prosthetic heart valve

NASA Astrophysics Data System (ADS)

Hasler, D.; Landolt, A.; Obrist, D.

2016-05-01

The instantaneous three-dimensional velocity field past a bioprosthetic heart valve was measured using tomographic particle image velocimetry. Two digital cameras were used together with a mirror setup to record PIV images from four different angles. Measurements were conducted in a transparent silicone phantom with a simplified geometry of the aortic root. The refraction indices of the silicone phantom and the working fluid were matched to minimize optical distortion from the flow field to the cameras. The silicone phantom of the aorta was integrated in a flow loop driven by a piston pump. Measurements were conducted for steady and pulsatile flow conditions. Results of the instantaneous, ensemble and phase-averaged flow field are presented. The three-dimensional velocity field reveals a flow topology, which can be related to features of the aortic valve prosthesis.

Measuring three-dimensional interaction potentials using optical interference.

PubMed

Mojarad, Nassir; Sandoghdar, Vahid; Krishnan, Madhavi

2013-04-22

We describe the application of three-dimensional (3D) scattering interferometric (iSCAT) imaging to the measurement of spatial interaction potentials for nano-objects in solution. We study electrostatically trapped gold particles in a nanofluidic device and present details on axial particle localization in the presence of a strongly reflecting interface. Our results demonstrate high-speed (~kHz) particle tracking with subnanometer localization precision in the axial and average 2.5 nm in the lateral dimension. A comparison of the measured levitation heights of trapped particles with the calculated values for traps of various geometries reveals good agreement. Our work demonstrates that iSCAT imaging delivers label-free, high-speed and accurate 3D tracking of nano-objects conducive to probing weak and long-range interaction potentials in solution.

Transparency-enhancing technology allows three-dimensional assessment of gastrointestinal mucosa: A porcine model.

PubMed

Mizutani, Hiroya; Ono, Satoshi; Ushiku, Tetsuo; Kudo, Yotaro; Ikemura, Masako; Kageyama, Natsuko; Yamamichi, Nobutake; Fujishiro, Mitsuhiro; Someya, Takao; Fukayama, Masashi; Koike, Kazuhiko; Onodera, Hiroshi

2018-02-01

Although high-resolution three-dimensional imaging of endoscopically resected gastrointestinal specimens can help elucidating morphological features of gastrointestinal mucosa or tumor, there are no established methods to achieve this without breaking specimens apart. We evaluated the utility of transparency-enhancing technology for three-dimensional assessment of gastrointestinal mucosa in porcine models. Esophagus, stomach, and colon mucosa samples obtained from a sacrificed swine were formalin-fixed and paraffin-embedded, and subsequently deparaffinized for analysis. The samples were fluorescently stained, optically cleared using transparency-enhancing technology: ilLUmination of Cleared organs to IDentify target molecules method (LUCID), and visualized using laser scanning microscopy. After observation, all specimens were paraffin-embedded again and evaluated by conventional histopathological assessment to measure the impact of transparency-enhancing procedures. As a result, microscopic observation revealed horizontal section views of mucosa at deeper levels and enabled the three-dimensional image reconstruction of glandular and vascular structures. Besides, paraffin-embedded specimens after transparency-enhancing procedures were all assessed appropriately by conventional histopathological staining. These results suggest that transparency-enhancing technology may be feasible for clinical application and enable the three-dimensional structural analysis of endoscopic resected specimen non-destructively. Although there remain many limitations or problems to be solved, this promising technology might represent a novel histopathological method for evaluating gastrointestinal cancers. © 2018 Japanese Society of Pathology and John Wiley & Sons Australia, Ltd.

Automated framework for intraretinal cystoid macular edema segmentation in three-dimensional optical coherence tomography images with macular hole

NASA Astrophysics Data System (ADS)

Zhu, Weifang; Zhang, Li; Shi, Fei; Xiang, Dehui; Wang, Lirong; Guo, Jingyun; Yang, Xiaoling; Chen, Haoyu; Chen, Xinjian

2017-07-01

Cystoid macular edema (CME) and macular hole (MH) are the leading causes for visual loss in retinal diseases. The volume of the CMEs can be an accurate predictor for visual prognosis. This paper presents an automatic method to segment the CMEs from the abnormal retina with coexistence of MH in three-dimensional-optical coherence tomography images. The proposed framework consists of preprocessing and CMEs segmentation. The preprocessing part includes denoising, intraretinal layers segmentation and flattening, and MH and vessel silhouettes exclusion. In the CMEs segmentation, a three-step strategy is applied. First, an AdaBoost classifier trained with 57 features is employed to generate the initialization results. Second, an automated shape-constrained graph cut algorithm is applied to obtain the refined results. Finally, cyst area information is used to remove false positives (FPs). The method was evaluated on 19 eyes with coexistence of CMEs and MH from 18 subjects. The true positive volume fraction, FP volume fraction, dice similarity coefficient, and accuracy rate for CMEs segmentation were 81.0%±7.8%, 0.80%±0.63%, 80.9%±5.7%, and 99.7%±0.1%, respectively.

Fluorescence laminar optical tomography for brain imaging: system implementation and performance evaluation.

PubMed

Azimipour, Mehdi; Sheikhzadeh, Mahya; Baumgartner, Ryan; Cullen, Patrick K; Helmstetter, Fred J; Chang, Woo-Jin; Pashaie, Ramin

2017-01-01

We present our effort in implementing a fluorescence laminar optical tomography scanner which is specifically designed for noninvasive three-dimensional imaging of fluorescence proteins in the brains of small rodents. A laser beam, after passing through a cylindrical lens, scans the brain tissue from the surface while the emission signal is captured by the epi-fluorescence optics and is recorded using an electron multiplication CCD sensor. Image reconstruction algorithms are developed based on Monte Carlo simulation to model light–tissue interaction and generate the sensitivity matrices. To solve the inverse problem, we used the iterative simultaneous algebraic reconstruction technique. The performance of the developed system was evaluated by imaging microfabricated silicon microchannels embedded inside a substrate with optical properties close to the brain as a tissue phantom and ultimately by scanning brain tissue in vivo. Details of the hardware design and reconstruction algorithms are discussed and several experimental results are presented. The developed system can specifically facilitate neuroscience experiments where fluorescence imaging and molecular genetic methods are used to study the dynamics of the brain circuitries.

Terrestrial Planet Finder Coronagraph Optical Modeling

NASA Technical Reports Server (NTRS)

Basinger, Scott A.; Redding, David C.

2004-01-01

The Terrestrial Planet Finder Coronagraph will rely heavily on modeling and analysis throughout its mission lifecycle. Optical modeling is especially important, since the tolerances on the optics as well as scattered light suppression are critical for the mission's success. The high contrast imaging necessary to observe a planet orbiting a distant star requires new and innovative technologies to be developed and tested, and detailed optical modeling provides predictions for evaluating design decisions. It also provides a means to develop and test algorithms designed to actively suppress scattered light via deformable mirrors and other techniques. The optical models are used in conjunction with structural and thermal models to create fully integrated optical/structural/thermal models that are used to evaluate dynamic effects of disturbances on the overall performance of the coronagraph. The optical models we have developed have been verified on the High Contrast Imaging Testbed. Results of the optical modeling verification and the methods used to perform full three-dimensional near-field diffraction analysis are presented.

Full-Color Plasmonic Metasurface Holograms.

PubMed

Wan, Weiwei; Gao, Jie; Yang, Xiaodong

2016-12-27

Holography is one of the most attractive approaches for reconstructing optical images, due to its capability of recording both the amplitude and phase information on light scattered from objects. Recently, optical metasurfaces for manipulating the wavefront of light with well-controlled amplitude, phase, and polarization have been utilized to reproduce computer-generated holograms. However, the currently available metasurface holograms have only been designed to achieve limited colors and record either amplitude or phase information. This fact significantly limits the performance of metasurface holograms to reconstruct full-color images with low noise and high quality. Here, we report the design and realization of ultrathin plasmonic metasurface holograms made of subwavelength nanoslits for reconstructing both two- and three-dimensional full-color holographic images. The wavelength-multiplexed metasurface holograms with both amplitude and phase modulations at subwavelength scale can faithfully produce not only three primary colors but also their secondary colors. Our results will advance various holographic applications.

Long working distance incoherent interference microscope

DOEpatents

Sinclair, Michael B [Albuquerque, NM; De Boer, Maarten P [Albuquerque, NM

2006-04-25

A full-field imaging, long working distance, incoherent interference microscope suitable for three-dimensional imaging and metrology of MEMS devices and test structures on a standard microelectronics probe station. A long working distance greater than 10 mm allows standard probes or probe cards to be used. This enables nanometer-scale 3-dimensional height profiles of MEMS test structures to be acquired across an entire wafer while being actively probed, and, optionally, through a transparent window. An optically identical pair of sample and reference arm objectives is not required, which reduces the overall system cost, and also the cost and time required to change sample magnifications. Using a LED source, high magnification (e.g., 50.times.) can be obtained having excellent image quality, straight fringes, and high fringe contrast.

The forensic holodeck: an immersive display for forensic crime scene reconstructions.

PubMed

Ebert, Lars C; Nguyen, Tuan T; Breitbeck, Robert; Braun, Marcel; Thali, Michael J; Ross, Steffen

2014-12-01

In forensic investigations, crime scene reconstructions are created based on a variety of three-dimensional image modalities. Although the data gathered are three-dimensional, their presentation on computer screens and paper is two-dimensional, which incurs a loss of information. By applying immersive virtual reality (VR) techniques, we propose a system that allows a crime scene to be viewed as if the investigator were present at the scene. We used a low-cost VR headset originally developed for computer gaming in our system. The headset offers a large viewing volume and tracks the user's head orientation in real-time, and an optical tracker is used for positional information. In addition, we created a crime scene reconstruction to demonstrate the system. In this article, we present a low-cost system that allows immersive, three-dimensional and interactive visualization of forensic incident scene reconstructions.

Multiphoton imaging microscopy at deeper layers with adaptive optics control of spherical aberration.

PubMed

Bueno, Juan M; Skorsetz, Martin; Palacios, Raquel; Gualda, Emilio J; Artal, Pablo

2014-01-01

Despite the inherent confocality and optical sectioning capabilities of multiphoton microscopy, three-dimensional (3-D) imaging of thick samples is limited by the specimen-induced aberrations. The combination of immersion objectives and sensorless adaptive optics (AO) techniques has been suggested to overcome this difficulty. However, a complex plane-by-plane correction of aberrations is required, and its performance depends on a set of image-based merit functions. We propose here an alternative approach to increase penetration depth in 3-D multiphoton microscopy imaging. It is based on the manipulation of the spherical aberration (SA) of the incident beam with an AO device while performing fast tomographic multiphoton imaging. When inducing SA, the image quality at best focus is reduced; however, better quality images are obtained from deeper planes within the sample. This is a compromise that enables registration of improved 3-D multiphoton images using nonimmersion objectives. Examples on ocular tissues and nonbiological samples providing different types of nonlinear signal are presented. The implementation of this technique in a future clinical instrument might provide a better visualization of corneal structures in living eyes.

LIPS database with LIPService: a microscopic image database of intracellular structures in Arabidopsis guard cells.

PubMed

Higaki, Takumi; Kutsuna, Natsumaro; Hasezawa, Seiichiro

2013-05-16

Intracellular configuration is an important feature of cell status. Recent advances in microscopic imaging techniques allow us to easily obtain a large number of microscopic images of intracellular structures. In this circumstance, automated microscopic image recognition techniques are of extreme importance to future phenomics/visible screening approaches. However, there was no benchmark microscopic image dataset for intracellular organelles in a specified plant cell type. We previously established the Live Images of Plant Stomata (LIPS) database, a publicly available collection of optical-section images of various intracellular structures of plant guard cells, as a model system of environmental signal perception and transduction. Here we report recent updates to the LIPS database and the establishment of a database table, LIPService. We updated the LIPS dataset and established a new interface named LIPService to promote efficient inspection of intracellular structure configurations. Cell nuclei, microtubules, actin microfilaments, mitochondria, chloroplasts, endoplasmic reticulum, peroxisomes, endosomes, Golgi bodies, and vacuoles can be filtered using probe names or morphometric parameters such as stomatal aperture. In addition to the serial optical sectional images of the original LIPS database, new volume-rendering data for easy web browsing of three-dimensional intracellular structures have been released to allow easy inspection of their configurations or relationships with cell status/morphology. We also demonstrated the utility of the new LIPS image database for automated organelle recognition of images from another plant cell image database with image clustering analyses. The updated LIPS database provides a benchmark image dataset for representative intracellular structures in Arabidopsis guard cells. The newly released LIPService allows users to inspect the relationship between organellar three-dimensional configurations and morphometrical parameters.

All-optical photochromic spatial light modulators based on photoinduced electron transfer in rigid matrices

NASA Technical Reports Server (NTRS)

Beratan, David N. (Inventor); Perry, Joseph W. (Inventor)

1991-01-01

A single material (not a multi-element structure) spatial light modulator may be written to, as well as read out from, using light. The device has tailorable rise and hold times dependent on the composition and concentration of the molecular species used as the active components. The spatial resolution of this device is limited only by light diffraction as in volume holograms. The device may function as a two-dimensional mask (transmission or reflection) or as a three-dimensional volume holographic medium. This device, based on optically-induced electron transfer, is able to perform incoherent to coherent image conversion or wavelength conversion over a wide spectral range (ultraviolet, visible, or near-infrared regions).

Three dimensional optic tissue culture and process

NASA Technical Reports Server (NTRS)

Spaulding, Glenn F. (Inventor); Prewett, Tacey L. (Inventor); Goodwin, Thomas J. (Inventor); Francis, Karen M. (Inventor); Cardwell, Delmar R. (Inventor); Oconnor, Kim (Inventor); Fitzgerald, Wendy S. (Inventor); Aten, Laurie A. (Inventor)

1994-01-01

A process for artificially producing three-dimensional optic tissue has been developed. The optic cells are cultured in a bioreactor at low shear conditions. The tissue forms normal, functional tissue organization and extracellular matrix.

The optical design concept of SPICA-SAFARI

NASA Astrophysics Data System (ADS)

Jellema, Willem; Kruizinga, Bob; Visser, Huib; van den Dool, Teun; Pastor Santos, Carmen; Torres Redondo, Josefina; Eggens, Martin; Ferlet, Marc; Swinyard, Bruce; Dohlen, Kjetil; Griffin, Doug; Gonzalez Fernandez, Luis Miguel; Belenguer, Tomas; Matsuhara, Hideo; Kawada, Mitsunobu; Doi, Yasuo

2012-09-01

The Safari instrument on the Japanese SPICA mission is a zodiacal background limited imaging spectrometer offering a photometric imaging (R ≍ 2), and a low (R = 100) and medium spectral resolution (R = 2000 at 100 μm) spectroscopy mode in three photometric bands covering the 34-210 μm wavelength range. The instrument utilizes Nyquist sampled filled arrays of very sensitive TES detectors providing a 2’x2’ instantaneous field of view. The all-reflective optical system of Safari is highly modular and consists of an input optics module containing the entrance shutter, a calibration source and a pair of filter wheels, followed by an interferometer and finally the camera bay optics accommodating the focal-plane arrays. The optical design is largely driven and constrained by volume inviting for a compact three-dimensional arrangement of the interferometer and camera bay optics without compromising the optical performance requirements associated with a diffraction- and background-limited spectroscopic imaging instrument. Central to the optics we present a flexible and compact non-polarizing Mach-Zehnder interferometer layout, with dual input and output ports, employing a novel FTS scan mechanism based on magnetic bearings and a linear motor. In this paper we discuss the conceptual design of the focal-plane optics and describe how we implement the optical instrument functions, define the photometric bands, deal with straylight control, diffraction and thermal emission in the long-wavelength limit and interface to the large-format FPA arrays at one end and the SPICA telescope assembly at the other end.

Three-Dimensional Orientation of Anisotropic Plasmonic Aggregates at Intracellular Nuclear Indentation Sites by Integrated Light Sheet Super-Resolution Microscopy.

PubMed

Chakkarapani, Suresh Kumar; Sun, Yucheng; Lee, Seungah; Fang, Ning; Kang, Seong Ho

2018-05-22

Three-dimensional (3D) orientations of individual anisotropic plasmonic nanoparticles in aggregates were observed in real time by integrated light sheet super-resolution microscopy ( iLSRM). Asymmetric light scattering of a gold nanorod (AuNR) was used to trigger signals based on the polarizer angle. Controlled photoswitching was achieved by turning the polarizer and obtaining a series of images at different polarization directions. 3D subdiffraction-limited super-resolution images were obtained by superlocalization of scattering signals as a function of the anisotropic optical properties of AuNRs. Varying the polarizer angle allowed resolution of the orientation of individual AuNRs. 3D images of individual nanoparticles were resolved in aggregated regions, resulting in as low as 64 nm axial resolution and 28 nm spatial resolution. The proposed imaging setup and localization approach demonstrates a convenient method for imaging under a noisy environment where the majority of scattering noise comes from cellular components. This integrated 3D iLSRM and localization technique was shown to be reliable and useful in the field of 3D nonfluorescence super-resolution imaging.

Fabrication of an Optical Fiber Micro-Sphere with a Diameter of Several Tens of Micrometers.

PubMed

Yu, Huijuan; Huang, Qiangxian; Zhao, Jian

2014-06-25

A new method to fabricate an integrated optical fiber micro-sphere with a diameter within 100 µm, based on the optical fiber tapering technique and the Taguchi method is proposed. Using a 125 µm diameter single-mode (SM) optical fiber, an optical fiber taper with a cone angle is formed with the tapering technique, and the fabrication optimization of a micro-sphere with a diameter of less than 100 µm is achieved using the Taguchi method. The optimum combination of process factors levels is obtained, and the signal-to-noise ratio (SNR) of three quality evaluation parameters and the significance of each process factors influencing them are selected as the two standards. Using the minimum zone method (MZM) to evaluate the quality of the fabricated optical fiber micro-sphere, a three-dimensional (3D) numerical fitting image of its surface profile and the true sphericity are subsequently realized. From the results, an optical fiber micro-sphere with a two-dimensional (2D) diameter less than 80 µm, 2D roundness error less than 0.70 µm, 2D offset distance between the micro-sphere center and the fiber stylus central line less than 0.65 µm, and true sphericity of about 0.5 µm, is fabricated.

Range and egomotion estimation from compound photodetector arrays with parallel optical axis using optical flow techniques.

PubMed

Chahl, J S

2014-01-20

This paper describes an application for arrays of narrow-field-of-view sensors with parallel optical axes. These devices exhibit some complementary characteristics with respect to conventional perspective projection or angular projection imaging devices. Conventional imaging devices measure rotational egomotion directly by measuring the angular velocity of the projected image. Translational egomotion cannot be measured directly by these devices because the induced image motion depends on the unknown range of the viewed object. On the other hand, a known translational motion generates image velocities which can be used to recover the ranges of objects and hence the three-dimensional (3D) structure of the environment. A new method is presented for computing egomotion and range using the properties of linear arrays of independent narrow-field-of-view optical sensors. An approximate parallel projection can be used to measure translational egomotion in terms of the velocity of the image. On the other hand, a known rotational motion of the paraxial sensor array generates image velocities, which can be used to recover the 3D structure of the environment. Results of tests of an experimental array confirm these properties.

High-speed optical coherence tomography by circular interferometric ranging

NASA Astrophysics Data System (ADS)

Siddiqui, Meena; Nam, Ahhyun S.; Tozburun, Serhat; Lippok, Norman; Blatter, Cedric; Vakoc, Benjamin J.

2018-02-01

Existing three-dimensional optical imaging methods excel in controlled environments, but are difficult to deploy over large, irregular and dynamic fields. This means that they can be ill-suited for use in areas such as material inspection and medicine. To better address these applications, we developed methods in optical coherence tomography to efficiently interrogate sparse scattering fields, that is, those in which most locations (voxels) do not generate meaningful signal. Frequency comb sources are used to superimpose reflected signals from equispaced locations through optical subsampling. This results in circular ranging, and reduces the number of measurements required to interrogate large volumetric fields. As a result, signal acquisition barriers that have limited speed and field in optical coherence tomography are avoided. With a new ultrafast, time-stretched frequency comb laser design operating with 7.6 MHz to 18.9 MHz repetition rates, we achieved imaging of multi-cm3 fields at up to 7.5 volumes per second.

Sub-micrometre accurate free-form optics by three-dimensional printing on single-mode fibres

PubMed Central

Gissibl, Timo; Thiele, Simon; Herkommer, Alois; Giessen, Harald

2016-01-01

Micro-optics are widely used in numerous applications, such as beam shaping, collimation, focusing and imaging. We use femtosecond 3D printing to manufacture free-form micro-optical elements. Our method gives sub-micrometre accuracy so that direct manufacturing even on single-mode fibres is possible. We demonstrate the potential of our method by writing different collimation optics, toric lenses, free-form surfaces with polynomials of up to 10th order for intensity beam shaping, as well as chiral photonic crystals for circular polarization filtering, all aligned onto the core of the single-mode fibres. We determine the accuracy of our optics by analysing the output patterns as well as interferometrically characterizing the surfaces. We find excellent agreement with numerical calculations. 3D printing of microoptics can achieve sufficient performance that will allow for rapid prototyping and production of beam-shaping and imaging devices. PMID:27339700

Sub-micrometre accurate free-form optics by three-dimensional printing on single-mode fibres.

PubMed

Gissibl, Timo; Thiele, Simon; Herkommer, Alois; Giessen, Harald

2016-06-24

Micro-optics are widely used in numerous applications, such as beam shaping, collimation, focusing and imaging. We use femtosecond 3D printing to manufacture free-form micro-optical elements. Our method gives sub-micrometre accuracy so that direct manufacturing even on single-mode fibres is possible. We demonstrate the potential of our method by writing different collimation optics, toric lenses, free-form surfaces with polynomials of up to 10th order for intensity beam shaping, as well as chiral photonic crystals for circular polarization filtering, all aligned onto the core of the single-mode fibres. We determine the accuracy of our optics by analysing the output patterns as well as interferometrically characterizing the surfaces. We find excellent agreement with numerical calculations. 3D printing of microoptics can achieve sufficient performance that will allow for rapid prototyping and production of beam-shaping and imaging devices.

Sub-micrometre accurate free-form optics by three-dimensional printing on single-mode fibres

NASA Astrophysics Data System (ADS)

Gissibl, Timo; Thiele, Simon; Herkommer, Alois; Giessen, Harald

2016-06-01

Micro-optics are widely used in numerous applications, such as beam shaping, collimation, focusing and imaging. We use femtosecond 3D printing to manufacture free-form micro-optical elements. Our method gives sub-micrometre accuracy so that direct manufacturing even on single-mode fibres is possible. We demonstrate the potential of our method by writing different collimation optics, toric lenses, free-form surfaces with polynomials of up to 10th order for intensity beam shaping, as well as chiral photonic crystals for circular polarization filtering, all aligned onto the core of the single-mode fibres. We determine the accuracy of our optics by analysing the output patterns as well as interferometrically characterizing the surfaces. We find excellent agreement with numerical calculations. 3D printing of microoptics can achieve sufficient performance that will allow for rapid prototyping and production of beam-shaping and imaging devices.

In situ industrial applications of optics; Proceedings of the Meeting, Brussels, Belgium, June 25-27, 1986

NASA Astrophysics Data System (ADS)

Ebbeni, Jean

Included in this volume are papers on real-time image enhancement by simple video systems, automatic identification and data collection via barcode laser scanning, the optimization of the cutting up of a strip of float glass, optical sensors for factory automation, and the use of a digital theodolite with infrared radiation. Attention is also given to ISIS (integrated shape imaging system), a new system for follow-up of scoliosis; optical diffraction extensometers; a cross-spectrum technique for high-sensitivity remote vibration analysis by optical interferometry; the compensation and measurement of any motion of three-dimensional objects in holographic interferometry; and stereoscreen. Additional papers are on holographic double pulse YAG lasers, miniature optic connectors, stress-field analysis in an adhesively bonded joint with laser photoelasticimetry, and the locking of the light pulse delay in externally triggered gas lasers.

Head Mounted Display with a Roof Mirror Array Fold

NASA Technical Reports Server (NTRS)

Olczak, Eugene (Inventor)

2014-01-01

The present invention includes a head mounted display (HMD) worn by a user. The HMD includes a display projecting an image through an optical lens. The HMD also includes a one-dimensional retro reflective array receiving the image through the optical lens at a first angle with respect to the display and deflecting the image at a second angle different than the first angle with respect to the display. The one-dimensional retro reflective array reflects the image in order to project the image onto an eye of the user.

4-D photoacoustic tomography.

PubMed

Xiang, Liangzhong; Wang, Bo; Ji, Lijun; Jiang, Huabei

2013-01-01

Photoacoustic tomography (PAT) offers three-dimensional (3D) structural and functional imaging of living biological tissue with label-free, optical absorption contrast. These attributes lend PAT imaging to a wide variety of applications in clinical medicine and preclinical research. Despite advances in live animal imaging with PAT, there is still a need for 3D imaging at centimeter depths in real-time. We report the development of four dimensional (4D) PAT, which integrates time resolutions with 3D spatial resolution, obtained using spherical arrays of ultrasonic detectors. The 4D PAT technique generates motion pictures of imaged tissue, enabling real time tracking of dynamic physiological and pathological processes at hundred micrometer-millisecond resolutions. The 4D PAT technique is used here to image needle-based drug delivery and pharmacokinetics. We also use this technique to monitor 1) fast hemodynamic changes during inter-ictal epileptic seizures and 2) temperature variations during tumor thermal therapy.

Optical imaging of oral pathological tissue using optical coherence tomography and synchrotron radiation computed microtomography

NASA Astrophysics Data System (ADS)

Cânjǎu, Silvana; Todea, Carmen; Sinescu, Cosmin; Negrutiu, Meda L.; Duma, Virgil; Mǎnescu, Adrian; Topalǎ, Florin I.; Podoleanu, Adrian Gh.

2013-06-01

The efforts aimed at early diagnosis of oral cancer should be prioritized towards developing a new screening instrument, based on optical coherence tomography (OCT), to be used directly intraorally, able to perform a fast, real time, 3D and non-invasive diagnosis of oral malignancies. The first step in this direction would be to optimize the OCT image interpretation of oral tissues. Therefore we propose plastination as a tissue preparation method that better preserves three-dimensional structure for study by new optical imaging techniques. The OCT and the synchrotron radiation computed microtomography (micro-CT) were employed for tissue sample analyze. For validating the OCT results we used the gold standard diagnostic procedure for any suspicious lesion - histopathology. This is a preliminary study of comparing features provided by OCT and Micro-CT. In the conditions of the present study, OCT proves to be a highly promising imaging modality. The use of x-ray based topographic imaging of small biological samples has been limited by the low intrinsic x-ray absorption of non-mineralized tissue and the lack of established contrast agents. Plastination can be used to enhance optical imagies of oral soft tissue samples.

Hadamard multimode optical imaging transceiver

DOEpatents

Cooke, Bradly J; Guenther, David C; Tiee, Joe J; Kellum, Mervyn J; Olivas, Nicholas L; Weisse-Bernstein, Nina R; Judd, Stephen L; Braun, Thomas R

2012-10-30

Disclosed is a method and system for simultaneously acquiring and producing results for multiple image modes using a common sensor without optical filtering, scanning, or other moving parts. The system and method utilize the Walsh-Hadamard correlation detection process (e.g., functions/matrix) to provide an all-binary structure that permits seamless bridging between analog and digital domains. An embodiment may capture an incoming optical signal at an optical aperture, convert the optical signal to an electrical signal, pass the electrical signal through a Low-Noise Amplifier (LNA) to create an LNA signal, pass the LNA signal through one or more correlators where each correlator has a corresponding Walsh-Hadamard (WH) binary basis function, calculate a correlation output coefficient for each correlator as a function of the corresponding WH binary basis function in accordance with Walsh-Hadamard mathematical principles, digitize each of the correlation output coefficient by passing each correlation output coefficient through an Analog-to-Digital Converter (ADC), and performing image mode processing on the digitized correlation output coefficients as desired to produce one or more image modes. Some, but not all, potential image modes include: multi-channel access, temporal, range, three-dimensional, and synthetic aperture.

A Review of Adaptive Optics Optical Coherence Tomography: Technical Advances, Scientific Applications, and the Future

PubMed Central

Jonnal, Ravi S.; Kocaoglu, Omer P.; Zawadzki, Robert J.; Liu, Zhuolin; Miller, Donald T.; Werner, John S.

2016-01-01

Purpose Optical coherence tomography (OCT) has enabled “virtual biopsy” of the living human retina, revolutionizing both basic retina research and clinical practice over the past 25 years. For most of those years, in parallel, adaptive optics (AO) has been used to improve the transverse resolution of ophthalmoscopes to foster in vivo study of the retina at the microscopic level. Here, we review work done over the last 15 years to combine the microscopic transverse resolution of AO with the microscopic axial resolution of OCT, building AO-OCT systems with the highest three-dimensional resolution of any existing retinal imaging modality. Methods We surveyed the literature to identify the most influential antecedent work, important milestones in the development of AO-OCT technology, its applications that have yielded new knowledge, research areas into which it may productively expand, and nascent applications that have the potential to grow. Results Initial efforts focused on demonstrating three-dimensional resolution. Since then, many improvements have been made in resolution and speed, as well as other enhancements of acquisition and postprocessing techniques. Progress on these fronts has produced numerous discoveries about the anatomy, function, and optical properties of the retina. Conclusions Adaptive optics OCT continues to evolve technically and to contribute to our basic and clinical knowledge of the retina. Due to its capacity to reveal cellular and microscopic detail invisible to clinical OCT systems, it is an ideal companion to those instruments and has the demonstrable potential to produce images that can guide the interpretation of clinical findings. PMID:27409507

Visualizing biofilm formation in endotracheal tubes using endoscopic three-dimensional optical coherence tomography

NASA Astrophysics Data System (ADS)

Heidari, Andrew E.; Moghaddam, Samer; Troung, Kimberly K.; Chou, Lidek; Genberg, Carl; Brenner, Matthew; Chen, Zhongping

2015-12-01

Biofilm formation has been linked to ventilator-associated pneumonia, which is a prevalent infection in hospital intensive care units. Currently, there is no rapid diagnostic tool to assess the degree of biofilm formation or cellular biofilm composition. Optical coherence tomography (OCT) is a minimally invasive, nonionizing imaging modality that can be used to provide high-resolution cross-sectional images. Biofilm deposited in critical care patients' endotracheal tubes was analyzed in vitro. This study demonstrates that OCT could potentially be used as a diagnostic tool to analyze and assess the degree of biofilm formation and extent of airway obstruction caused by biofilm in endotracheal tubes.

Long-range time-of-flight scanning sensor based on high-speed time-correlated single-photon counting

DOE Office of Scientific and Technical Information (OSTI.GOV)

McCarthy, Aongus; Collins, Robert J.; Krichel, Nils J.

2009-11-10

We describe a scanning time-of-flight system which uses the time-correlated single-photon counting technique to produce three-dimensional depth images of distant, noncooperative surfaces when these targets are illuminated by a kHz to MHz repetition rate pulsed laser source. The data for the scene are acquired using a scanning optical system and an individual single-photon detector. Depth images have been successfully acquired with centimeter xyz resolution, in daylight conditions, for low-signature targets in field trials at distances of up to 325 m using an output illumination with an average optical power of less than 50 {mu}W.

Conjugate adaptive optics with remote focusing in multiphoton microscopy

NASA Astrophysics Data System (ADS)

Tao, Xiaodong; Lam, Tuwin; Zhu, Bingzhao; Li, Qinggele; Reinig, Marc R.; Kubby, Joel

2018-02-01

The small correction volume for conventional wavefront shaping methods limits their application in biological imaging through scattering media. In this paper, we take advantage of conjugate adaptive optics (CAO) and remote focusing (CAORF) to achieve three-dimensional (3D) scanning through a scattering layer with a single correction. Our results show that the proposed system can provide 10 times wider axial field of view compared with a conventional conjugate AO system when 16,384 segments are used on a spatial light modulator. We demonstrate two-photon imaging with CAORF through mouse skull. The fluorescent microspheres embedded under the scattering layers can be clearly observed after applying the correction.

Imaging deep skeletal muscle structure using a high-sensitivity ultrathin side-viewing optical coherence tomography needle probe

PubMed Central

Yang, Xiaojie; Lorenser, Dirk; McLaughlin, Robert A.; Kirk, Rodney W.; Edmond, Matthew; Simpson, M. Cather; Grounds, Miranda D.; Sampson, David D.

2013-01-01

We have developed an extremely miniaturized optical coherence tomography (OCT) needle probe (outer diameter 310 µm) with high sensitivity (108 dB) to enable minimally invasive imaging of cellular structure deep within skeletal muscle. Three-dimensional volumetric images were acquired from ex vivo mouse tissue, examining both healthy and pathological dystrophic muscle. Individual myofibers were visualized as striations in the images. Degradation of cellular structure in necrotic regions was seen as a loss of these striations. Tendon and connective tissue were also visualized. The observed structures were validated against co-registered hematoxylin and eosin (H&E) histology sections. These images of internal cellular structure of skeletal muscle acquired with an OCT needle probe demonstrate the potential of this technique to visualize structure at the microscopic level deep in biological tissue in situ. PMID:24466482

Three-dimensional multi bioluminescent sources reconstruction based on adaptive finite element method

NASA Astrophysics Data System (ADS)

Ma, Xibo; Tian, Jie; Zhang, Bo; Zhang, Xing; Xue, Zhenwen; Dong, Di; Han, Dong

2011-03-01

Among many optical molecular imaging modalities, bioluminescence imaging (BLI) has more and more wide application in tumor detection and evaluation of pharmacodynamics, toxicity, pharmacokinetics because of its noninvasive molecular and cellular level detection ability, high sensitivity and low cost in comparison with other imaging technologies. However, BLI can not present the accurate location and intensity of the inner bioluminescence sources such as in the bone, liver or lung etc. Bioluminescent tomography (BLT) shows its advantage in determining the bioluminescence source distribution inside a small animal or phantom. Considering the deficiency of two-dimensional imaging modality, we developed three-dimensional tomography to reconstruct the information of the bioluminescence source distribution in transgenic mOC-Luc mice bone with the boundary measured data. In this paper, to study the osteocalcin (OC) accumulation in transgenic mOC-Luc mice bone, a BLT reconstruction method based on multilevel adaptive finite element (FEM) algorithm was used for localizing and quantifying multi bioluminescence sources. Optical and anatomical information of the tissues are incorporated as a priori knowledge in this method, which can reduce the ill-posedness of BLT. The data was acquired by the dual modality BLT and Micro CT prototype system that was developed by us. Through temperature control and absolute intensity calibration, a relative accurate intensity can be calculated. The location of the OC accumulation was reconstructed, which was coherent with the principle of bone differentiation. This result also was testified by ex vivo experiment in the black 96-plate well using the BLI system and the chemiluminescence apparatus.

Performance evaluation of an improved optical computed tomography polymer gel dosimeter system for 3D dose verification of static and dynamic phantom deliveries

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lopatiuk-Tirpak, O.; Langen, K. M.; Meeks, S. L.

2008-09-15

The performance of a next-generation optical computed tomography scanner (OCTOPUS-5X) is characterized in the context of three-dimensional gel dosimetry. Large-volume (2.2 L), muscle-equivalent, radiation-sensitive polymer gel dosimeters (BANG-3) were used. Improvements in scanner design leading to shorter acquisition times are discussed. The spatial resolution, detectable absorbance range, and reproducibility are assessed. An efficient method for calibrating gel dosimeters using the depth-dose relationship is applied, with photon- and electron-based deliveries yielding equivalent results. A procedure involving a preirradiation scan was used to reduce the edge artifacts in reconstructed images, thereby increasing the useful cross-sectional area of the dosimeter by nearly amore » factor of 2. Dose distributions derived from optical density measurements using the calibration coefficient show good agreement with the treatment planning system simulations and radiographic film measurements. The feasibility of use for motion (four-dimensional) dosimetry is demonstrated on an example comparing dose distributions from static and dynamic delivery of a single-field photon plan. The capability to visualize three-dimensional dose distributions is also illustrated.« less

Optical stereo video signal processor

NASA Technical Reports Server (NTRS)

Craig, G. D. (Inventor)

1985-01-01

An otpical video signal processor is described which produces a two-dimensional cross-correlation in real time of images received by a stereo camera system. The optical image of each camera is projected on respective liquid crystal light valves. The images on the liquid crystal valves modulate light produced by an extended light source. This modulated light output becomes the two-dimensional cross-correlation when focused onto a video detector and is a function of the range of a target with respect to the stereo camera. Alternate embodiments utilize the two-dimensional cross-correlation to determine target movement and target identification.

Optical Microangiography Based on Optical Coherence Tomography

NASA Astrophysics Data System (ADS)

Reif, Roberto; Wang, Ruikang K.

Proper homeostasis regulation of in vivo biological systems requires microvascular blood perfusion, which is the process of delivering blood into the tissue's capillary beds. Abnormal tissue vascularization has been associated with various diseases such as cancer, diabetes, neurological disorders, wounds, and inflammation. Understanding the changes in the vascular network or microangiography will have an important role in determining the causes and developing potential treatments for these diseases. Optical coherence tomography (OCT) is a noninvasive method for imaging three-dimensional biological tissues with high resolution (~10 µm) and without requiring the use of contrast agents. In this chapter we review several techniques for using OCT to determine blood flow velocities and the vessel morphology (optical microangiography). Different techniques will be discussed with a brief explanation of their limitations. Also, methods for quantifying these images are presented, as well as the depiction of several applications.

Nonlinear optical microscopy reveals invading endothelial cells anisotropically alter three-dimensional collagen matrices

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lee, P.-F.; Yeh, Alvin T.; Bayless, Kayla J.

The interactions between endothelial cells (ECs) and the extracellular matrix (ECM) are fundamental in mediating various steps of angiogenesis, including cell adhesion, migration and sprout formation. Here, we used a noninvasive and non-destructive nonlinear optical microscopy (NLOM) technique to optically image endothelial sprouting morphogenesis in three-dimensional (3D) collagen matrices. We simultaneously captured signals from collagen fibers and endothelial cells using second harmonic generation (SHG) and two-photon excited fluorescence (TPF), respectively. Dynamic 3D imaging revealed EC interactions with collagen fibers along with quantifiable alterations in collagen matrix density elicited by EC movement through and morphogenesis within the matrix. Specifically, we observedmore » increased collagen density in the area between bifurcation points of sprouting structures and anisotropic increases in collagen density around the perimeter of lumenal structures, but not advancing sprout tips. Proteinase inhibition studies revealed membrane-associated matrix metalloproteinase were utilized for sprout advancement and lumen expansion. Rho-associated kinase (p160ROCK) inhibition demonstrated that the generation of cell tension increased collagen matrix alterations. This study followed sprouting ECs within a 3D matrix and revealed that the advancing structures recognize and significantly alter their extracellular environment at the periphery of lumens as they progress.« less

Validation of two-dimensional and three-dimensional measurements of subpleural alveolar size parameters by optical coherence tomography

PubMed Central

Warger, William C.; Hostens, Jeroen; Namati, Eman; Birngruber, Reginald; Bouma, Brett E.; Tearney, Guillermo J.

2012-01-01

Abstract. Optical coherence tomography (OCT) has been increasingly used for imaging pulmonary alveoli. Only a few studies, however, have quantified individual alveolar areas, and the validity of alveolar volumes represented within OCT images has not been shown. To validate quantitative measurements of alveoli from OCT images, we compared the cross-sectional area, perimeter, volume, and surface area of matched subpleural alveoli from microcomputed tomography (micro-CT) and OCT images of fixed air-filled swine samples. The relative change in size between different alveoli was extremely well correlated (r>0.9, P<0.0001), but OCT images underestimated absolute sizes compared to micro-CT by 27% (area), 7% (perimeter), 46% (volume), and 25% (surface area) on average. We hypothesized that the differences resulted from refraction at the tissue–air interfaces and developed a ray-tracing model that approximates the reconstructed alveolar size within OCT images. Using this model and OCT measurements of the refractive index for lung tissue (1.41 for fresh, 1.53 for fixed), we derived equations to obtain absolute size measurements of superellipse and circular alveoli with the use of predictive correction factors. These methods and results should enable the quantification of alveolar sizes from OCT images in vivo. PMID:23235834

Validation of two-dimensional and three-dimensional measurements of subpleural alveolar size parameters by optical coherence tomography

NASA Astrophysics Data System (ADS)

Unglert, Carolin I.; Warger, William C.; Hostens, Jeroen; Namati, Eman; Birngruber, Reginald; Bouma, Brett E.; Tearney, Guillermo J.

2012-12-01

Optical coherence tomography (OCT) has been increasingly used for imaging pulmonary alveoli. Only a few studies, however, have quantified individual alveolar areas, and the validity of alveolar volumes represented within OCT images has not been shown. To validate quantitative measurements of alveoli from OCT images, we compared the cross-sectional area, perimeter, volume, and surface area of matched subpleural alveoli from microcomputed tomography (micro-CT) and OCT images of fixed air-filled swine samples. The relative change in size between different alveoli was extremely well correlated (r>0.9, P<0.0001), but OCT images underestimated absolute sizes compared to micro-CT by 27% (area), 7% (perimeter), 46% (volume), and 25% (surface area) on average. We hypothesized that the differences resulted from refraction at the tissue-air interfaces and developed a ray-tracing model that approximates the reconstructed alveolar size within OCT images. Using this model and OCT measurements of the refractive index for lung tissue (1.41 for fresh, 1.53 for fixed), we derived equations to obtain absolute size measurements of superellipse and circular alveoli with the use of predictive correction factors. These methods and results should enable the quantification of alveolar sizes from OCT images in vivo.

Optical image encryption using chaos-based compressed sensing and phase-shifting interference in fractional wavelet domain

NASA Astrophysics Data System (ADS)

Liu, Qi; Wang, Ying; Wang, Jun; Wang, Qiong-Hua

2018-02-01

In this paper, a novel optical image encryption system combining compressed sensing with phase-shifting interference in fractional wavelet domain is proposed. To improve the encryption efficiency, the volume data of original image are decreased by compressed sensing. Then the compacted image is encoded through double random phase encoding in asymmetric fractional wavelet domain. In the encryption system, three pseudo-random sequences, generated by three-dimensional chaos map, are used as the measurement matrix of compressed sensing and two random-phase masks in the asymmetric fractional wavelet transform. It not only simplifies the keys to storage and transmission, but also enhances our cryptosystem nonlinearity to resist some common attacks. Further, holograms make our cryptosystem be immune to noises and occlusion attacks, which are obtained by two-step-only quadrature phase-shifting interference. And the compression and encryption can be achieved in the final result simultaneously. Numerical experiments have verified the security and validity of the proposed algorithm.

Development of compact integral field unit for spaceborne solar spectro-polarimeter

NASA Astrophysics Data System (ADS)

Suematsu, Y.; Koyama, M.; Sukegawa, T.; Enokida, Y.; Saito, K.; Okura, Y.; Nakayasu, T.; Ozaki, S.; Tsuneta, S.

2017-11-01

A 1.5-m class aperture Solar Ultra-violet Visible and IR telescope (SUVIT) and its instruments for the Japanese next space solar mission SOLAR-C [1] are under study to obtain critical physical parameters in the lower solar atmosphere. For the precise magnetic field measurements covering field-of-view of 3 arcmin x3 acmin, a full stokes polarimetry at three magnetic sensitive lines in wavelength range of 525 nm to 1083 nm with a four-slit spectrograph of two dinesional image scanning mechanism is proposed: one is a true slit and the other three are pseudo-slits from integral field unit (IFU). To suit this configuration, besides a fiber bundle IFU, a compact mirror slicer IFU is designed and being developed. Integral field spectroscopy (IFS), which is realized with IFU, is a two dimensional spectroscopy, providing spectra simultaneously for each spatial direction of an extended two-dimensional field. The scientific advantages of the IFS for studies of localized and transient solar surface phenomena are obvious. There are in general three methods [2][3] to realize the IFS depending on image slicing devices such as a micro-lenslet array, an optical fiber bundle and a narrow rectangular image slicer array. So far, there exist many applications of the IFS for ground-based astronomical observations [4]. Regarding solar instrumentations, the IFS of micro-lenslet array was done by Suematsu et al. [5], the IFS of densely packed rectangular fiber bundle with thin clads was realized [6] and being developed for 4-m aperture solar telescope DKIST by Lin [7] and being considered for space solar telescope SOLAR-C by Katsukawa et al. [8], and the IFS with mirror slicer array was presented by Ren et al. [9] and under study for up-coming large-aperture solar telescope in Europe by Calcines et al. [10] From the view point of a high efficiency spectroscopy, a wide wavelength coverage, a precision spectropolarimetry and space application, the image slicer consisting of all reflective optics is the best option among the three. However, the image slicers are presently limited either by their risk in the case of classical glass polishing techniques (see Vivès et al. [11] for recent development) or by their optical performances when constituted by metallic mirrors. For space instruments, small sized units are much advantageous and demands that width of each slicer mirror is as narrow as an optimal slit width (< 100 micron) of spectrograph which is usually hard to manufacture with glass polishing techniques. On the other hand, Canon is developing a novel technique for such as high performance gratings which can be applicable for manufacturing high optical performance metallic mirrors of small dimensions. For the space-borne spectrograph of SUVIT to be aboard SOLAR-C, we designed the IFS made of a micro image slicer of 45 arrayed 30-micron-thick metal mirrors and a pseudo-pupil metal mirror array re-formatting three pseudo-slits; the design is feasible for optical configuration sharing a spectrograph with a conventional real slit. According to the optical deign, Canon manufactured a prototype IFU for evaluation, demonstrating high performances of micro image slicer and pupil mirrors; enough small micro roughness for visible light spectrographs, sharp edges for efficient image slices, surface figure for high image quality, etc. In the following, we describe the optical design of IFU feasible for space-borne spectrograph, manufacturing method to attain high optical performance of metal mirrors developed by Canon, and resulted performance of prototype IFU in detail.

Volumetric structured illumination microscopy enabled by tunable focus lens (Conference Presentation)

NASA Astrophysics Data System (ADS)

Hinsdale, Taylor; Malik, Bilal; Olsovsky, Cory; Jo, Javier A.; Maitland, Kristen C.

2016-03-01

We present a volumetric imaging method for biological tissue that is free of mechanically scanning components. The optical sectioning in the system is obtained by structured illumination microscopy (SIM) with the depth of focus being varied by the use of an electronic tunable-focus lens (ETL). The performance of the axial scanning mechanism was evaluated and characterized in conjunction with SIM to ensure volumetric images could be recorded and reconstructed without significant losses in optical section thickness and lateral resolution over the full desired scan range. It was demonstrated that sub-cellular image resolutions were obtainable in both microsphere films and in ex vivo oral mucosa, spanning multiple cell layers, without significant losses in image quality. The mechanism proposed here has the ability to be integrated into any wide-field microscopy system to convert it into a three-dimensional imaging platform without the need for axial scanning of the sample or imaging optics. The ability to axially scan independent of mechanical movement also provides the opportunity for the development of endoscopic systems which can create volumetric images of tissue in vivo.

Ganglion cell-inner plexiform layer and retinal nerve fiber layer thickness according to myopia and optic disc area: a quantitative and three-dimensional analysis.

PubMed

Seo, Sam; Lee, Chong Eun; Jeong, Jae Hoon; Park, Ki Ho; Kim, Dong Myung; Jeoung, Jin Wook

2017-03-11

To determine the influences of myopia and optic disc size on ganglion cell-inner plexiform layer (GCIPL) and peripapillary retinal nerve fiber layer (RNFL) thickness profiles obtained by spectral domain optical coherence tomography (OCT). One hundred and sixty-eight eyes of 168 young myopic subjects were recruited and assigned to one of three groups according to their spherical equivalent (SE) values and optic disc area. All underwent Cirrus HD-OCT imaging. The influences of myopia and optic disc size on the GCIPL and RNFL thickness profiles were evaluated by multiple comparisons and linear regression analysis. Three-dimensional surface plots of GCIPL and RNFL thickness corresponding to different combinations of myopia and optic disc size were constructed. Each of the quadrant RNFL thicknesses and their overall average were significantly thinner in high myopia compared to low myopia, except for the temporal quadrant (all Ps ≤0.003). The average and all-sectors GCIPL were significantly thinner in high myopia than in moderate- and/or low-myopia (all Ps ≤0.002). The average OCT RNFL thickness was correlated significantly with SE (0.81 μm/diopter, P < 0.001), axial length (-1.44 μm/mm, P < 0.001), and optic disc area (5.35 μm/mm 2 , P < 0.001) by linear regression analysis. As for the OCT GCIPL parameters, average GCIPL thickness showed a significant correlation with SE (0.84 μm/diopter, P < 0.001) and axial length (-1.65 μm/mm, P < 0.001). There was no significant correlation of average GCIPL thickness with optic disc area. Three-dimensional curves showed that larger optic discs were associated with increased average RNFL thickness and that more-myopic eyes were associated with decreased average GCIPL and RNFL thickness. Myopia can significantly affect GCIPL and RNFL thickness profiles, and optic disc size has a significant influence on RNFL thickness. The current OCT maps employed in the evaluation of glaucoma should be analyzed in consideration of refractive status and optic disc size.

In vivo and ex vivo imaging with ultrahigh resolution full-field OCT

NASA Astrophysics Data System (ADS)

Grieve, Kate; Moneron, Gael; Schwartz, Wilfrid; Boccara, Albert C.; Dubois, Arnaud

2005-08-01

Imaging of in vivo and ex vivo biological samples using full-field optical coherence tomography is demonstrated. Three variations on the original full-field optical coherence tomography instrument are presented, and evaluated in terms of performance. The instruments are based on the Linnik interferometer illuminated by a white light source. Images in the en face orientation are obtained in real-time without scanning by using a two-dimensional parallel detector array. An isotropic resolution capability better than 1 μm is achieved thanks to the use of a broad spectrum source and high numerical aperture microscope objectives. Detection sensitivity up to 90 dB is demonstrated. Image acquisition times as short as 10 μs per en face image are possible. A variety of in vivo and ex vivo imaging applications is explored, particularly in the fields of embryology, ophthalmology and botany.

Optical microscope and tapered fiber coupling apparatus for a dilution refrigerator.

PubMed

MacDonald, A J R; Popowich, G G; Hauer, B D; Kim, P H; Fredrick, A; Rojas, X; Doolin, P; Davis, J P

2015-01-01

We have developed a system for tapered fiber measurements of optomechanical resonators inside a dilution refrigerator, which is compatible with both on- and off-chip devices. Our apparatus features full three-dimensional control of the taper-resonator coupling conditions enabling critical coupling, with an overall fiber transmission efficiency of up to 70%. Notably, our design incorporates an optical microscope system consisting of a coherent bundle of 37,000 optical fibers for real-time imaging of the experiment at a resolution of ∼1 μm. We present cryogenic optical and optomechanical measurements of resonators coupled to tapered fibers at temperatures as low as 9 mK.

Maximum-performance fiber-optic irradiation with nonimaging designs.

PubMed

Fang, Y; Feuermann, D; Gordon, J M

1997-10-01

A range of practical nonimaging designs for optical fiber applications is presented. Rays emerging from a fiber over a restricted angular range (small numerical aperture) are needed to illuminate a small near-field detector at maximum radiative efficiency. These designs range from pure reflector (all-mirror), to pure dielectric (refractive and based on total internal reflection) to lens-mirror combinations. Sample designs are shown for a specific infrared fiber-optic irradiation problem of practical interest. Optical performance is checked with computer three-dimensional ray tracing. Compared with conventional imaging solutions, nonimaging units offer considerable practical advantages in compactness and ease of alignment as well as noticeably superior radiative efficiency.

Three Dimensional Reconstruction Algorithm for Imaging Pathophysiological Signals Within Breast Tissue Using Near Infrared Light

DTIC Science & Technology

2006-07-01

months.31 A heated mixture of water, gelatin (G2625, Sigma Inc.), India ink (for absorption), and titanium dioxide powder (for scatter) (TiO2, Sigma Inc...for absorption, and titanium dioxide powder for scat- ter TiO2, Sigma Inc. that are solidified by cooling to room temperature. Optically...2713-2727. 8. Bolin, F.P., Preuss, L. E., Taylor, R. C., Ference, R. J, Refractive index of some mammalian tissue using a fiber optic cladding method

Expert Systems for the Scheduling of Image Processing Tasks on a Parallel Processing System

DTIC Science & Technology

1986-12-01

existed for over twenty years. Credit for designing and implementing the first computer vision system is usually given to L. G . Roberts [Robe65]. With...hardware differences between systems. 44 LIST OF REFERENCES [Adam82] G . B. Adams III and H. J. Siegel, "The Extra Stage Cube: a Fault-Tolerant...Academic Press, 1985 [Robe65] L. G . Roberts, "Machine Perception of Three-Dimensional Solids," in Optical and Electro-Optical Information Processing, ed. J

Photoacoustic microscopy of human teeth

NASA Astrophysics Data System (ADS)

Rao, Bin; Cai, Xin; Favazza, Christopher; Yao, Junjie; Li, Li; Duong, Steven; Liaw, Lih-Huei; Holtzman, Jennifer; Wilder-Smith, Petra; Wang, Lihong V.

2011-03-01

Photoacoustic microscopy (PAM) utilizes short laser pulses to deposit energy into light absorbers and sensitively detects the ultrasonic waves the absorbers generate in response. PAM directly renders a three-dimensional spatial distribution of sub-surface optical absorbers. Unlike other optical imaging technologies, PAM features label-free optical absorption contrast and excellent imaging depths. Standard dental imaging instruments are limited to X-ray and CCD cameras. Subsurface optical dental imaging is difficult due to the highly-scattering enamel and dentin tissue. Thus, very few imaging methods can detect dental decay or diagnose dental pulp, which is the innermost part of the tooth, containing the nerves, blood vessels, and other cells. Here, we conducted a feasibility study on imaging dental decay and dental pulp with PAM. Our results showed that PAM is sensitive to the color change associated with dental decay. Although the relative PA signal distribution may be affected by surface contours and subsurface reflections from deeper dental tissue, monitoring changes in the PA signals (at the same site) over time is necessary to identify the progress of dental decay. Our results also showed that deep-imaging, near-infrared (NIR) PAM can sensitively image blood in the dental pulp of an in vitro tooth. In conclusion, PAM is a promising tool for imaging both dental decay and dental pulp.

Micro-optical design of a three-dimensional microlens scanner for vertically integrated micro-opto-electro-mechanical systems.

PubMed

Baranski, Maciej; Bargiel, Sylwester; Passilly, Nicolas; Gorecki, Christophe; Jia, Chenping; Frömel, Jörg; Wiemer, Maik

2015-08-01

This paper presents the optical design of a miniature 3D scanning system, which is fully compatible with the vertical integration technology of micro-opto-electro-mechanical systems (MOEMS). The constraints related to this integration strategy are considered, resulting in a simple three-element micro-optical setup based on an afocal scanning microlens doublet and a focusing microlens, which is tolerant to axial position inaccuracy. The 3D scanning is achieved by axial and lateral displacement of microlenses of the scanning doublet, realized by micro-electro-mechanical systems microactuators (the transmission scanning approach). Optical scanning performance of the system is determined analytically by use of the extended ray transfer matrix method, leading to two different optical configurations, relying either on a ball lens or plano-convex microlenses. The presented system is aimed to be a core component of miniature MOEMS-based optical devices, which require a 3D optical scanning function, e.g., miniature imaging systems (confocal or optical coherence microscopes) or optical tweezers.

Optimal Anesthetic Regime for Motionless Three-Dimensional Image Acquisition During Longitudinal Studies of Adult Nonpigmented Zebrafish.

PubMed

Lockwood, Nicola; Parker, Jennifer; Wilson, Carole; Frankel, Paul

2017-04-01

With many live imaging techniques, it is crucial that a deep level of anesthesia is reached and maintained throughout image acquisition without reducing zebrafish viability. This is particularly true for three-dimensional tomographic imaging modalities. Currently, the most commonly used anesthetic in the zebrafish community, MS-222 (tricaine methanesulfonate), does not allow this. We show, using a combination of both MS-222 and isoflurane, that we can significantly improve the anesthetic regime required for motionless image acquisition of live adult zebrafish. We have benchmarked this against the requirements of our novel quantitative imaging platform, compressive sensing optical projection tomography. Using nonpigmented transgenic zebrafish, we show that a combination of 175 ppm of both anesthetics improves the maintenance of deep anesthesia for prolonged periods of time and it can be used repeatedly to enable longitudinal imaging. Importantly, it does not affect the health or viability of the adult zebrafish. We also show that nonpigmented fish, with a mutated form of the gene transparent, took significantly longer to reach deep anesthesia. The anesthetic regime presented in this study should lead to significant improvements in accuracy and information achievable from imaging live adult zebrafish and in its application to longitudinal studies.

A review on noise suppression and aberration compensation in holographic particle image velocimetry

NASA Astrophysics Data System (ADS)

Tamrin, K. F.; Rahmatullah, B.

2016-12-01

Understanding three-dimensional (3D) fluid flow behaviour is undeniably crucial in improving performance and efficiency in a wide range of applications in engineering and medical fields. Holographic particle image velocimetry (HPIV) is a potential tool to probe and characterize complex flow dynamics since it is a truly three-dimensional three-component measurement technique. The technique relies on the coherent light scattered by small seeding particles that are assumed to faithfully follow the flow for subsequent reconstruction of the same the event afterward. However, extraction of useful 3D displacement data from these particle images is usually aggravated by noise and aberration which are inherent within the optical system. Noise and aberration have been considered as major hurdles in HPIV in obtaining accurate particle image identification and its corresponding 3D position. Major contributions to noise include zero-order diffraction, out-of-focus particles, virtual image and emulsion grain scattering. Noise suppression is crucial to ensure that particle image can be distinctly differentiated from background noise while aberration compensation forms particle image with high integrity. This paper reviews a number of HPIV configurations that have been proposed to address these issues, summarizes the key findings and outlines a basis for follow-on research.

3D printed phantoms of retinal photoreceptor cells for evaluating adaptive optics imaging modalities

NASA Astrophysics Data System (ADS)

Kedia, Nikita; Liu, Zhuolin; Sochol, Ryan; Hammer, Daniel X.; Agrawal, Anant

2018-02-01

Adaptive optics-enabled optical coherence tomography (AO-OCT) and scanning laser ophthalmoscopy (AO-SLO) devices can resolve retinal cones and rods in three dimensions. To evaluate the improved resolution of AO-OCT and AO-SLO, a phantom that mimics retinal anatomy at the cellular level is required. We used a two-photon polymerization approach to fabricate three-dimensional (3D) photoreceptor phantoms modeled on the central foveal cones. By using a femtosecond laser to selectively photocure precise locations within a liquid-based photoresist via two-photon absorption, we produced high-resolution phantoms with μm-level dimensions similar to true anatomy. In this work, we present two phantoms to evaluate the resolution limits of an AO imaging system: one that models only the outer segments of the photoreceptor cells at varying retinal eccentricities and another that contains anatomically relevant features of the full-length photoreceptor. With these phantoms we are able to quantitatively estimate transverse resolution of an AO system and produce images that are comparable to those found in the human retina.

CυBE: Coherent υ Beam Educator

NASA Astrophysics Data System (ADS)

Sureshkumar, Vivian Amos; Richardson, M.

2017-03-01

Holography has advanced rapidly over the years due to technical melioration in the field of optics. Three-dimensional imaging has gained importance to upgrade the existing imaging and display system. Holography has become one of the branches of optics gaining significant importance with a vast number of technical and industrial applications. When we address holography the first thing that comes to mind is projecting a three dimensional object on thin air. The word holography has always been confused between peppers ghost effect. The famous English phrase "A picture is worth a thousand words", means a complex idea can be conveyed by a single picture. The basic principle of holography sounds complex with all its technical terms. This paper aims to explain the concept of the CυBE: Coherent υ Beam Educator that contains a transmission hologram illuminated with a laser diode. This paper summarizes the construction details of the CυBE and the optical setup to record the transmission hologram. It also briefs the circuit connections for the laser diode that's works with an aid of a push button. When viewer presses the push button the original scene is reconstructed. It provides details regarding the angle of reference beam at recording and how the reference beam is compensated at reconstruction. Also this paper highlights how the magnification of the recorded image is affected with respect to the path length of the laser diode inside the box during reconstruction of the recorded hologram.

Choriocapillaris and Choroidal Microvasculature Imaging with Ultrahigh Speed OCT Angiography

PubMed Central

Choi, WooJhon; Mohler, Kathrin J.; Potsaid, Benjamin; Lu, Chen D.; Liu, Jonathan J.; Jayaraman, Vijaysekhar; Cable, Alex E.; Duker, Jay S.; Huber, Robert; Fujimoto, James G.

2013-01-01

We demonstrate in vivo choriocapillaris and choroidal microvasculature imaging in normal human subjects using optical coherence tomography (OCT). An ultrahigh speed swept source OCT prototype at 1060 nm wavelengths with a 400 kHz A-scan rate is developed for three-dimensional ultrahigh speed imaging of the posterior eye. OCT angiography is used to image three-dimensional vascular structure without the need for exogenous fluorophores by detecting erythrocyte motion contrast between OCT intensity cross-sectional images acquired rapidly and repeatedly from the same location on the retina. En face OCT angiograms of the choriocapillaris and choroidal vasculature are visualized by acquiring cross-sectional OCT angiograms volumetrically via raster scanning and segmenting the three-dimensional angiographic data at multiple depths below the retinal pigment epithelium (RPE). Fine microvasculature of the choriocapillaris, as well as tightly packed networks of feeding arterioles and draining venules, can be visualized at different en face depths. Panoramic ultra-wide field stitched OCT angiograms of the choriocapillaris spanning ∼32 mm on the retina show distinct vascular structures at different fundus locations. Isolated smaller fields at the central fovea and ∼6 mm nasal to the fovea at the depths of the choriocapillaris and Sattler's layer show vasculature structures consistent with established architectural morphology from histological and electron micrograph corrosion casting studies. Choriocapillaris imaging was performed in eight healthy volunteers with OCT angiograms successfully acquired from all subjects. These results demonstrate the feasibility of ultrahigh speed OCT for in vivo dye-free choriocapillaris and choroidal vasculature imaging, in addition to conventional structural imaging. PMID:24349078

Choriocapillaris and choroidal microvasculature imaging with ultrahigh speed OCT angiography.

PubMed

Choi, WooJhon; Mohler, Kathrin J; Potsaid, Benjamin; Lu, Chen D; Liu, Jonathan J; Jayaraman, Vijaysekhar; Cable, Alex E; Duker, Jay S; Huber, Robert; Fujimoto, James G

2013-01-01

We demonstrate in vivo choriocapillaris and choroidal microvasculature imaging in normal human subjects using optical coherence tomography (OCT). An ultrahigh speed swept source OCT prototype at 1060 nm wavelengths with a 400 kHz A-scan rate is developed for three-dimensional ultrahigh speed imaging of the posterior eye. OCT angiography is used to image three-dimensional vascular structure without the need for exogenous fluorophores by detecting erythrocyte motion contrast between OCT intensity cross-sectional images acquired rapidly and repeatedly from the same location on the retina. En face OCT angiograms of the choriocapillaris and choroidal vasculature are visualized by acquiring cross-sectional OCT angiograms volumetrically via raster scanning and segmenting the three-dimensional angiographic data at multiple depths below the retinal pigment epithelium (RPE). Fine microvasculature of the choriocapillaris, as well as tightly packed networks of feeding arterioles and draining venules, can be visualized at different en face depths. Panoramic ultra-wide field stitched OCT angiograms of the choriocapillaris spanning ∼32 mm on the retina show distinct vascular structures at different fundus locations. Isolated smaller fields at the central fovea and ∼6 mm nasal to the fovea at the depths of the choriocapillaris and Sattler's layer show vasculature structures consistent with established architectural morphology from histological and electron micrograph corrosion casting studies. Choriocapillaris imaging was performed in eight healthy volunteers with OCT angiograms successfully acquired from all subjects. These results demonstrate the feasibility of ultrahigh speed OCT for in vivo dye-free choriocapillaris and choroidal vasculature imaging, in addition to conventional structural imaging.

Parametric approaches to micro-scale characterization of tissue volumes in vivo and ex vivo: Imaging microvasculature, attenuation, birefringence, and stiffness (Conference Presentation)

NASA Astrophysics Data System (ADS)

Sampson, David D.; Chin, Lixin; Gong, Peijun; Wijesinghe, Philip; Es'haghian, Shaghayegh; Allen, Wesley M.; Klyen, Blake R.; Kirk, Rodney W.; Kennedy, Brendan F.; McLaughlin, Robert A.

2016-03-01

INVITED TALK Advances in imaging tissue microstructure in living subjects, or in freshly excised tissue with minimum preparation and processing, are important for future diagnosis and surgical guidance in the clinical setting, particularly for application to cancer. Whilst microscopy methods continue to advance on the cellular scale and medical imaging is well established on the scale of the whole tumor or organ, it is attractive to consider imaging the tumor environment on the micro-scale, between that of cells and whole tissues. Such a scenario is ideally suited to optical coherence tomography (OCT), with the twin attractions of requiring little or no tissue preparation, and in vivo capability. OCT's intrinsic scattering contrast reveals many morphological features of tumors, but is frequently ineffective in revealing other important aspects, such as microvasculature, or in reliably distinguishing tumor from uninvolved stroma. To address these shortcomings, we are developing several advances on the basic OCT approach. We are exploring speckle fluctuations to image tissue microvasculature and we have been developing several parametric approaches to tissue micro-scale characterization. Our approaches extract, from a three-dimensional OCT data set, a two-dimensional image of an optical parameter, such as attenuation or birefringence, or a mechanical parameter, such as stiffness, that aids in characterizing the tissue. This latter method, termed optical coherence elastography, parallels developments in ultrasound and magnetic resonance imaging. Parametric imaging of birefringence and of stiffness both show promise in addressing the important issue of differentiating cancer from uninvolved stroma in breast tissue.

Making 3D movies of Northern Lights

NASA Astrophysics Data System (ADS)

Hivon, Eric; Mouette, Jean; Legault, Thierry

2017-10-01

We describe the steps necessary to create three-dimensional (3D) movies of Northern Lights or Aurorae Borealis out of real-time images taken with two distant high-resolution fish-eye cameras. Astrometric reconstruction of the visible stars is used to model the optical mapping of each camera and correct for it in order to properly align the two sets of images. Examples of the resulting movies can be seen at http://www.iap.fr/aurora3d

Three-dimensional localization and optical imaging of objects in turbid media with independent component analysis.

PubMed

Xu, M; Alrubaiee, M; Gayen, S K; Alfano, R R

2005-04-01

A new approach for optical imaging and localization of objects in turbid media that makes use of the independent component analysis (ICA) from information theory is demonstrated. Experimental arrangement realizes a multisource illumination of a turbid medium with embedded objects and a multidetector acquisition of transmitted light on the medium boundary. The resulting spatial diversity and multiple angular observations provide robust data for three-dimensional localization and characterization of absorbing and scattering inhomogeneities embedded in a turbid medium. ICA of the perturbations in the spatial intensity distribution on the medium boundary sorts out the embedded objects, and their locations are obtained from Green's function analysis based on any appropriate light propagation model. Imaging experiments were carried out on two highly scattering samples of thickness approximately 50 times the transport mean-free path of the respective medium. One turbid medium had two embedded absorptive objects, and the other had four scattering objects. An independent component separation of the signal, in conjunction with diffusive photon migration theory, was used to locate the embedded inhomogeneities. In both cases, improved lateral and axial localizations of the objects over the result obtained by use of common photon migration reconstruction algorithms were achieved. The approach is applicable to different medium geometries, can be used with any suitable photon propagation model, and is amenable to near-real-time imaging applications.

Three Dimensional Optic Tissue Culture and Process

NASA Technical Reports Server (NTRS)

OConnor, Kim C. (Inventor); Spaulding, Glenn F. (Inventor); Goodwin, Thomas J. (Inventor); Aten, Laurie A. (Inventor); Francis, Karen M. (Inventor); Caldwell, Delmar R. (Inventor); Prewett, Tacey L. (Inventor); Fitzgerald, Wendy S. (Inventor)

1999-01-01

A process for artificially producing three-dimensional optic tissue has been developed. The optic cells are cultured in a bioireactor at low shear conditions. The tissue forms as normal, functional tissue grows with tissue organization and extracellular matrix formation.

BIM-Sim: Interactive Simulation of Broadband Imaging Using Mie Theory

PubMed Central

Berisha, Sebastian; van Dijk, Thomas; Bhargava, Rohit; Carney, P. Scott; Mayerich, David

2017-01-01

Understanding the structure of a scattered electromagnetic (EM) field is critical to improving the imaging process. Mechanisms such as diffraction, scattering, and interference affect an image, limiting the resolution, and potentially introducing artifacts. Simulation and visualization of scattered fields thus plays an important role in imaging science. However, EM fields are high-dimensional, making them time-consuming to simulate, and difficult to visualize. In this paper, we present a framework for interactively computing and visualizing EM fields scattered by micro and nano-particles. Our software uses graphics hardware for evaluating the field both inside and outside of these particles. We then use Monte-Carlo sampling to reconstruct and visualize the three-dimensional structure of the field, spectral profiles at individual points, the structure of the field at the surface of the particle, and the resulting image produced by an optical system. PMID:29170738

Multi-channel infrared thermometer

DOEpatents

Ulrickson, M.A.

A device for measuring the two-dimensional temperature profile of a surface comprises imaging optics for generating an image of the light radiating from the surface; an infrared detector array having a plurality of detectors; and optical means positioned between the imaging optics and the detector array for sampling, transmitting, and distributing the image over the detector surfaces. The optical means may be a light pipe array having one light pipe for each detector in the detector array.

Flat dielectric metasurface lens array for three dimensional integral imaging

NASA Astrophysics Data System (ADS)

Zhang, Jianlei; Wang, Xiaorui; Yang, Yi; Yuan, Ying; Wu, Xiongxiong

2018-05-01

In conventional integral imaging, the singlet refractive lens array limits the imaging performance due to its prominent aberrations. Different from the refractive lens array relying on phase modulation via phase change accumulated along the optical paths, metasurfaces composed of nano-scatters can produce phase abrupt over the scale of wavelength. In this letter, we propose a novel lens array consisting of two neighboring flat dielectric metasurfaces for integral imaging system. The aspherical phase profiles of the metasurfaces are optimized to improve imaging performance. The simulation results show that our designed 5 × 5 metasurface-based lens array exhibits high image quality at designed wavelength 865 nm.

The Three-Dimensional Point Spread Function of Aberration-Corrected Scanning Transmission Electron Microscopy

PubMed Central

Lupini, A.R.; de Jonge, N.

2012-01-01

Aberration-correction reduces the depth of field in scanning transmission electron microscopy (STEM) and thus allows three-dimensional imaging by depth-sectioning. This imaging mode offers the potential for sub-Ångstrom lateral resolution and nanometer-scale depth sensitivity. For biological samples, which may be many microns across and where high lateral resolution may not always be needed, optimizing the depth resolution even at the expense of lateral resolution may be desired, aiming to image through thick specimens. Although there has been extensive work examining and optimizing the probe formation in two-dimensions, there is less known about the probe shape along the optical axis. Here the probe shape is examined in three-dimensions in an attempt to better understand the depth-resolution in this mode. Examples are presented of how aberrations change the probe shape in three-dimensions, and it is found that off-axial aberrations may need to be considered for focal series of large areas. It is shown that oversized or annular apertures theoretically improve the vertical resolution for 3D imaging of nanoparticles. When imaging nanoparticles of several nanometer size, regular STEM can thereby be optimized such that the vertical full width at half maximum approaches that of the aberration corrected STEM with a standard aperture. PMID:21878149

Three-dimensional all-dielectric metamaterial solid immersion lens for subwavelength imaging at visible frequencies

PubMed Central

Fan, Wen; Yan, Bing; Wang, Zengbo; Wu, Limin

2016-01-01

Although all-dielectric metamaterials offer a low-loss alternative to current metal-based metamaterials to manipulate light at the nanoscale and may have important applications, very few have been reported to date owing to the current nanofabrication technologies. We develop a new “nano–solid-fluid assembly” method using 15-nm TiO2 nanoparticles as building blocks to fabricate the first three-dimensional (3D) all-dielectric metamaterial at visible frequencies. Because of its optical transparency, high refractive index, and deep-subwavelength structures, this 3D all-dielectric metamaterial-based solid immersion lens (mSIL) can produce a sharp image with a super-resolution of at least 45 nm under a white-light optical microscope, significantly exceeding the classical diffraction limit and previous near-field imaging techniques. Theoretical analysis reveals that electric field enhancement can be formed between contacting TiO2 nanoparticles, which causes effective confinement and propagation of visible light at the deep-subwavelength scale. This endows the mSIL with unusual abilities to illuminate object surfaces with large-area nanoscale near-field evanescent spots and to collect and convert the evanescent information into propagating waves. Our all-dielectric metamaterial design strategy demonstrates the potential to develop low-loss nanophotonic devices at visible frequencies. PMID:27536727

Methods for Dichoptic Stimulus Presentation in Functional Magnetic Resonance Imaging - A Review

PubMed Central

Choubey, Bhaskar; Jurcoane, Alina; Muckli, Lars; Sireteanu, Ruxandra

2009-01-01

Dichoptic stimuli (different stimuli displayed to each eye) are increasingly being used in functional brain imaging experiments using visual stimulation. These studies include investigation into binocular rivalry, interocular information transfer, three-dimensional depth perception as well as impairments of the visual system like amblyopia and stereodeficiency. In this paper, we review various approaches of displaying dichoptic stimulus used in functional magnetic resonance imaging experiments. These include traditional approaches of using filters (red-green, red-blue, polarizing) with optical assemblies as well as newer approaches of using bi-screen goggles. PMID:19526076

Acceleration of integral imaging based incoherent Fourier hologram capture using graphic processing unit.

PubMed

Jeong, Kyeong-Min; Kim, Hee-Seung; Hong, Sung-In; Lee, Sung-Keun; Jo, Na-Young; Kim, Yong-Soo; Lim, Hong-Gi; Park, Jae-Hyeung

2012-10-08

Speed enhancement of integral imaging based incoherent Fourier hologram capture using a graphic processing unit is reported. Integral imaging based method enables exact hologram capture of real-existing three-dimensional objects under regular incoherent illumination. In our implementation, we apply parallel computation scheme using the graphic processing unit, accelerating the processing speed. Using enhanced speed of hologram capture, we also implement a pseudo real-time hologram capture and optical reconstruction system. The overall operation speed is measured to be 1 frame per second.

Feasibility study of an optically coherent telescope array in space

NASA Technical Reports Server (NTRS)

Traub, W. A.

1983-01-01

Numerical methods of image construction which can be used to produce very high angular resolution images at optical wavelengths of astronomical objects from an orbiting array of telescopes are discussed and a concept is presented for a phase-coherent optical telescope array which may be deployed by space shuttle in the 1990's. The system would start as a four-element linear array with a 12 m baseline. The initial module is a minimum redundant array with a photon-counting collecting area three times larger than space telescope and a one dimensional resolution of better than 0.01 arc seconds in the visible range. Later additions to the array would build up facility capability. The advantages of a VLBI observatory in space are considered as well as apertures for the telescopes.

Automated fiber tracking and tissue characterization of the anterior cruciate ligament with optical coherence tomography

NASA Astrophysics Data System (ADS)

Balasubramanian, Priya S.; Guo, Jiaqi; Yao, Xinwen; Qu, Dovina; Lu, Helen H.; Hendon, Christine P.

2017-02-01

The directionality of collagen fibers across the anterior cruciate ligament (ACL) as well as the insertion of this key ligament into bone are important for understanding the mechanical integrity and functionality of this complex tissue. Quantitative analysis of three-dimensional fiber directionality is of particular interest due to the physiological, mechanical, and biological heterogeneity inherent across the ACL-to-bone junction, the behavior of the ligament under mechanical stress, and the usefulness of this information in designing tissue engineered grafts. We have developed an algorithm to characterize Optical Coherence Tomography (OCT) image volumes of the ACL. We present an automated algorithm for measuring ligamentous fiber angles, and extracting attenuation and backscattering coefficients of ligament, interface, and bone regions within mature and immature bovine ACL insertion samples. Future directions include translating this algorithm for real time processing to allow three-dimensional volumetric analysis within dynamically moving samples.

Self-assembled three-dimensional nanocrown array.

PubMed

Hong, Soongweon; Kang, Taewook; Choi, Dukhyun; Choi, Yeonho; Lee, Luke P

2012-07-24

Although an ordered nanoplasmonic probe array will have a huge impact on light harvesting, selective frequency response (i.e., nanoantenna), and quantitative molecular/cellular imaging, the realization of such an array is still limited by conventional techniques due to the serial processing or resolution limit by light diffraction. Here, we demonstrate a thermodynamically driven, self-assembled three-dimensional nanocrown array that consists of a core and six satellite gold nanoparticles (GNPs). Our ordered nanoprobe array is fabricated over a large area by thermal dewetting of thin gold film on hexagonally ordered porous anodic alumina (PAA). During thermal dewetting, the structural order of the PAA template dictates the periodic arrangement of gold nanoparticles, rendering the array of gold nanocrown. Because of its tunable size (i.e., 50 nm core and 20 nm satellite GNPs), arrangement, and periodicity, the nanocrown array shows multiple optical resonance frequencies at visible wavelengths as well as angle-dependent optical properties.

Three-dimensional polarization algebra.

PubMed

R Sheppard, Colin J; Castello, Marco; Diaspro, Alberto

2016-10-01

If light is focused or collected with a high numerical aperture lens, as may occur in imaging and optical encryption applications, polarization should be considered in three dimensions (3D). The matrix algebra of polarization behavior in 3D is discussed. It is useful to convert between the Mueller matrix and two different Hermitian matrices, representing an optical material or system, which are in the literature. Explicit transformation matrices for converting the column vector form of these different matrices are extended to the 3D case, where they are large (81×81) but can be generated using simple rules. It is found that there is some advantage in using a generalization of the Chandrasekhar phase matrix treatment, rather than that based on Gell-Mann matrices, as the resultant matrices are of simpler form and reduce to the two-dimensional case more easily. Explicit expressions are given for 3D complex field components in terms of Chandrasekhar-Stokes parameters.

High resolution imaging and lithography with hard x rays using parabolic compound refractive lenses

NASA Astrophysics Data System (ADS)

Schroer, C. G.; Benner, B.; Günzler, T. F.; Kuhlmann, M.; Zimprich, C.; Lengeler, B.; Rau, C.; Weitkamp, T.; Snigirev, A.; Snigireva, I.; Appenzeller, J.

2002-03-01

Parabolic compound refractive lenses are high quality optical components for hard x rays. They are particularly suited for full field imaging, with applications in microscopy and x-ray lithography. Taking advantage of the large penetration depth of hard x rays, the interior of opaque samples can be imaged with submicrometer resolution. To obtain the three-dimensional structure of a sample, microscopy is combined with tomographic techniques. In a first hard x-ray lithography experiment, parabolic compound refractive lenses have been used to project the reduced image of a lithography mask onto a resist. Future developments are discussed.

Comparative assessment of astigmatism-corrected Czerny-Turner imaging spectrometer using off-the-shelf optics

NASA Astrophysics Data System (ADS)

Yuan, Qun; Zhu, Dan; Chen, Yueyang; Guo, Zhenyan; Zuo, Chao; Gao, Zhishan

2017-04-01

We present the optical design of a Czerny-Turner imaging spectrometer for which astigmatism is corrected using off-the-shelf optics resulting in spectral resolution of 0.1 nm. The classic Czerny-Turner imaging spectrometer, consisting of a plane grating, two spherical mirrors, and a sensor with 10-μm pixels, was used as the benchmark. We comparatively assessed three configurations of the spectrometer that corrected astigmatism with divergent illumination of the grating, by adding a cylindrical lens, or by adding a cylindrical mirror. When configured with the added cylindrical lens, the imaging spectrometer with a point field of view (FOV) and a linear sensor achieved diffraction-limited performance over a broadband width of 400 nm centered at 800 nm, while the maximum allowable bandwidth was only 200 nm for the other two configurations. When configured with the added cylindrical mirror, the imaging spectrometer with a one-dimensional field of view (1D FOV) and an area sensor showed its superiority on imaging quality, spectral nonlinearity, as well as keystone over 100 nm bandwidth and 10 mm spatial extent along the entrance slit.

In vivo imaging of human oral hard and soft tissues by polarization-sensitive optical coherence tomography

NASA Astrophysics Data System (ADS)

Walther, Julia; Golde, Jonas; Kirsten, Lars; Tetschke, Florian; Hempel, Franz; Rosenauer, Tobias; Hannig, Christian; Koch, Edmund

2017-12-01

Since optical coherence tomography (OCT) provides three-dimensional high-resolution images of biological tissue, the benefit of polarization contrast in the field of dentistry is highlighted in this study. Polarization-sensitive OCT (PS OCT) with phase-sensitive recording is used for imaging dental and mucosal tissues in the human oral cavity in vivo. An enhanced polarization contrast of oral structures is reached by analyzing the signals of the co- and crosspolarized channels of the swept source PS OCT system quantitatively with respect to reflectivity, retardation, optic axis orientation, and depolarization. The calculation of these polarization parameters enables a high tissue-specific contrast imaging for the detailed physical interpretation of human oral hard and soft tissues. For the proof-of-principle, imaging of composite restorations and mineralization defects at premolars as well as gingival, lingual, and labial oral mucosa was performed in vivo within the anterior oral cavity. The achieved contrast-enhanced results of the investigated human oral tissues by means of polarization-sensitive imaging are evaluated by the comparison with conventional intensity-based OCT.

Dual-modal three-dimensional imaging of single cells with isometric high resolution using an optical projection tomography microscope

NASA Astrophysics Data System (ADS)

Miao, Qin; Rahn, J. Richard; Tourovskaia, Anna; Meyer, Michael G.; Neumann, Thomas; Nelson, Alan C.; Seibel, Eric J.

2009-11-01

The practice of clinical cytology relies on bright-field microscopy using absorption dyes like hematoxylin and eosin in the transmission mode, while the practice of research microscopy relies on fluorescence microscopy in the epi-illumination mode. The optical projection tomography microscope is an optical microscope that can generate 3-D images of single cells with isometric high resolution both in absorption and fluorescence mode. Although the depth of field of the microscope objective is in the submicron range, it can be extended by scanning the objective's focal plane. The extended depth of field image is similar to a projection in a conventional x-ray computed tomography. Cells suspended in optical gel flow through a custom-designed microcapillary. Multiple pseudoprojection images are taken by rotating the microcapillary. After these pseudoprojection images are further aligned, computed tomography methods are applied to create 3-D reconstruction. 3-D reconstructed images of single cells are shown in both absorption and fluorescence mode. Fluorescence spatial resolution is measured at 0.35 μm in both axial and lateral dimensions. Since fluorescence and absorption images are taken in two different rotations, mechanical error may cause misalignment of 3-D images. This mechanical error is estimated to be within the resolution of the system.

Alternatives for Ground-Based, Large-Aperture Optical Space Surveillance Systems

DTIC Science & Technology

2013-09-01

with a collection of information if it does not display a currently valid OMB control number. 1. REPORT DATE SEP 2013 2. REPORT TYPE 3. DATES COVERED...be replaced with a filter if required. Distribution A: Approved for Public Release, Distribution Unlimited 3 Fig. 1. Three dimensional...most advanced, wide-field survey telescopes in the world . The telescope images a patch of sky three times larger than that covered by GEODSS and

Optical penetration-based silkworm pupa gender sensor structure.

PubMed

Sumriddetchkajorn, Sarun; Kamtongdee, Chakkrit

2012-02-01

This paper proposes and experimentally demonstrates for what is believed to be the first time a highly sought-after optical structure for highly-accurate identification of the silkworm pupa gender. The key idea is to exploit a long wavelength optical beam in the red or near infrared spectrum that can effectively and safely penetrate the body of a silkworm pupa. Later on, simple image processing operations via image thresholding, blob filtering, and image inversion processes are applied in order to eliminate the unwanted image noises and at the same time highlight the gender gland. Experimental proof of concept using three 636 nm wavelength light emitting diodes, a two-dimensional web camera, an 8 bit microcontroller board, and a notebook computer shows a very high 95.6% total accuracy in identifying the gender of 45 silkworm pupae with a measured fast identification time of 96.6 ms. Other key features include low cost, low component counts, and ease of implementation and control.

Optical Inspection In Hostile Industrial Environments: Single-Sensor VS. Imaging Methods

NASA Astrophysics Data System (ADS)

Cielo, P.; Dufour, M.; Sokalski, A.

1988-11-01

On-line and unsupervised industrial inspection for quality control and process monitoring is increasingly required in the modern automated factory. Optical techniques are particularly well suited to industrial inspection in hostile environments because of their noncontact nature, fast response time and imaging capabilities. Optical sensors can be used for remote inspection of high temperature products or otherwise inaccessible parts, provided they are in a line-of-sight relation with the sensor. Moreover, optical sensors are much easier to adapt to a variety of part shapes, position or orientation and conveyor speeds as compared to contact-based sensors. This is an important requirement in a flexible automation environment. A number of choices are possible in the design of optical inspection systems. General-purpose two-dimensional (2-D) or three-dimensional (3-D) imaging techniques have advanced very rapidly in the last years thanks to a substantial research effort as well as to the availability of increasingly powerful and affordable hardware and software. Imaging can be realized using 2-D arrays or simpler one-dimensional (1-D) line-array detectors. Alternatively, dedicated single-spot sensors require a smaller amount of data processing and often lead to robust sensors which are particularly appropriate to on-line operation in hostile industrial environments. Many specialists now feel that dedicated sensors or clusters of sensors are often more effective for specific industrial automation and control tasks, at least in the short run. This paper will discuss optomechanical and electro-optical choices with reference to the design of a number of on-line inspection sensors which have been recently developed at our institute. Case studies will include real-time surface roughness evaluation on polymer cables extruded at high speed, surface characterization of hot-rolled or galvanized-steel sheets, temperature evaluation and pinhole detection in aluminum foil, multi-wavelength polymer sheet thickness gauging and thermographic imaging, 3-D lumber profiling, line-array inspection of textiles and glassware, as well as on-line optical inspection for the control of automated arc welding. In each case the design choices between single or multiple-element detectors, mechanical vs. electronic scanning, laser vs. incoherent illumination, etc. will be discussed in terms of industrial constraints such as speed requirements, protection against the environment or reliability of the sensor output.

PREFACE: Ultrafast biophotonics Ultrafast biophotonics

NASA Astrophysics Data System (ADS)

Gu, Min; Reid, Derryck; Ben-Yakar, Adela

2010-08-01

The use of light to explore biology can be traced to the first observations of tissue made with early microscopes in the mid-seventeenth century, and has today evolved into the discipline which we now know as biophotonics. This field encompasses a diverse range of activities, each of which shares the common theme of exploiting the interaction of light with biological material. With the rapid advancement of ultrafast optical technologies over the last few decades, ultrafast lasers have increasingly found applications in biophotonics, to the extent that the distinctive new field of ultrafast biophotonics has now emerged, where robust turnkey ultrafast laser systems are facilitating cutting-edge studies in the life sciences to take place in everyday laboratories. The broad spectral bandwidths, precision timing resolution, low coherence and high peak powers of ultrafast optical pulses provide unique opportunities for imaging and manipulating biological systems. Time-resolved studies of bio-molecular dynamics exploit the short pulse durations from such lasers, while other applications such as optical coherence tomography benefit from the broad optical bandwidths possible by using super-continuum generation and additionally allowing for high speed imaging with speeds as high as 47 000 scans per second. Continuing progress in laser-system technology is accelerating the adoption of ultrafast techniques across the life sciences, both in research laboratories and in clinical applications, such as laser-assisted in situ keratomileusis (LASIK) eye surgery. Revolutionizing the field of optical microscopy, two-photon excitation fluorescence (TPEF) microscopy has enabled higher spatial resolution with improved depth penetration into biological specimens. Advantages of this nonlinear optical process include: reduced photo-interactions, allowing for extensive imaging time periods; simultaneously exciting multiple fluorescent molecules with only one excitation wavelength; and reduced chromatic aberration effects. These extensive advantages have led to further exploration of nonlinear processes including second-harmonic generation (SHG) microscopy and third-harmonic generation (THG) microscopy. Second-harmonic generation has provided biologists with an extremely powerful tool for generating contrast in biological imaging, with the additional benefit of non-invasive three-dimensional imaging. The recent popularity of THG microscopy is largely due to the fact that three-dimensional imaging is achievable without the need for any labels, but rather relying on the intrinsic properties of the biological specimen itself. This optical nonlinear technique has attracted much attention recently from the biological community due to its non-invasive capabilities. Users of ultrafast lasers in the biological and medical fields are becoming a fast-growing community, employing pulse-shaping microscopy, resolution-enhancing microscopy techniques, linear and nonlinear micro-spectroscopy, functional deep-tissue imaging, optical coherence tomography, nonlinear fluorescence microscopy, molecular imaging and control, harmonic microscopy and femtosecond lifetime imaging, for cutting-edge research concerning the interaction of light with biological dynamics. The adaptability of ultrafast lasers to interact with a large array of materials through nonlinear excitation has enabled precise control of laser fluence allowing for highly localized material interactions, permitting micro-structured fabricated surfaces. The resultant multi-dimensional fabricated micro-structures are capable of replicating and/or manipulating microenvironments for controlled cell biology. In this special issue of Journal of Optics readers have a chance to view a collection of new contributions to the growing research field of ultrafast biophotonics. They are presented with recent advances in ultrafast technology applied to biological and medical investigations, where topics include advances in the visualization and identification of photo-reaction dynamics of biological functions under relevant physiological conditions, theoretically proposed imaging designs for obtaining super-resolved optical sectioned images in single exposures and fabricated micro-structured surfaces for biological micro-environments. We hope the collection will stimulate innovative new research in this growing field by showcasing new techniques for the visualization and manipulation of complex biological systems using linear and and nonlinear optical processes. Professor Min Gu would like to acknowledge Dr Betty Kouskousis for her contribution and support towards this editorial.

[Three-dimensional reconstruction of functional brain images].

PubMed

Inoue, M; Shoji, K; Kojima, H; Hirano, S; Naito, Y; Honjo, I

1999-08-01

We consider PET (positron emission tomography) measurement with SPM (Statistical Parametric Mapping) analysis to be one of the most useful methods to identify activated areas of the brain involved in language processing. SPM is an effective analytical method that detects markedly activated areas over the whole brain. However, with the conventional presentations of these functional brain images, such as horizontal slices, three directional projection, or brain surface coloring, makes understanding and interpreting the positional relationships among various brain areas difficult. Therefore, we developed three-dimensionally reconstructed images from these functional brain images to improve the interpretation. The subjects were 12 normal volunteers. The following three types of images were constructed: 1) routine images by SPM, 2) three-dimensional static images, and 3) three-dimensional dynamic images, after PET images were analyzed by SPM during daily dialog listening. The creation of images of both the three-dimensional static and dynamic types employed the volume rendering method by VTK (The Visualization Toolkit). Since the functional brain images did not include original brain images, we synthesized SPM and MRI brain images by self-made C++ programs. The three-dimensional dynamic images were made by sequencing static images with available software. Images of both the three-dimensional static and dynamic types were processed by a personal computer system. Our newly created images showed clearer positional relationships among activated brain areas compared to the conventional method. To date, functional brain images have been employed in fields such as neurology or neurosurgery, however, these images may be useful even in the field of otorhinolaryngology, to assess hearing and speech. Exact three-dimensional images based on functional brain images are important for exact and intuitive interpretation, and may lead to new developments in brain science. Currently, the surface model is the most common method of three-dimensional display. However, the volume rendering method may be more effective for imaging regions such as the brain.

High-speed upper-airway imaging using full-range optical coherence tomography

NASA Astrophysics Data System (ADS)

Jing, Joseph; Zhang, Jun; Loy, Anthony Chin; Wong, Brian J. F.; Chen, Zhongping

2012-11-01

Obstruction in the upper airway can often cause reductions in breathing or gas exchange efficiency and lead to rest disorders such as sleep apnea. Imaging diagnosis of the obstruction region has been accomplished using computed tomography (CT) and magnetic resonance imaging (MRI). However CT requires the use of ionizing radiation, and MRI typically requires sedation of the patient to prevent motion artifacts. Long-range optical coherence tomography (OCT) has the potential to provide high-speed three-dimensional tomographic images with high resolution and without the use of ionizing radiation. In this paper, we present work on the development of a long-range OCT endoscopic probe with 1.2 mm OD and 20 mm working distance used in conjunction with a modified Fourier domain swept source OCT system to acquire structural and anatomical datasets of the human airway. Imaging from the bottom of the larynx to the end of the nasal cavity is completed within 40 s.

Imaging of murine embryonic cardiovascular development using optical coherence tomography (Conference Presentation)

NASA Astrophysics Data System (ADS)

Huang, Yongyang; Degenhardt, Karl R.; Astrof, Sophie; Zhou, Chao

2016-03-01

We have demonstrated the capability of spectral domain optical coherence tomography (SDOCT) system to image full development of mouse embryonic cardiovascular system. Monitoring morphological changes of mouse embryonic heart occurred in different embryonic stages helps identify structural or functional cardiac anomalies and understand how these anomalies lead to congenital heart diseases (CHD) present at birth. In this study, mouse embryo hearts ranging from E9.5 to E15.5 were prepared and imaged in vitro. A customized spectral domain OCT system was used for imaging, with a central wavelength of 1310nm, spectral bandwidth of ~100nm and imaging speed of 47kHz A-scans/s. Axial resolution of this system was 8.3µm in air, and transverse resolution was 6.2 µm with 5X objective. Key features of mouse embryonic cardiovascular development such as vasculature remodeling into circulatory system, separation of atria and ventricles and emergence of valves could be clearly seen in three-dimensional OCT images. Optical clearing was applied to overcome the penetration limit of OCT system. With high resolution, fast imaging speed, 3D imaging capability, OCT proves to be a promising biomedical imaging modality for developmental biology studies, rivaling histology and micro-CT.

Defect inspection of actuator lenses using swept-source optical coherence tomography

NASA Astrophysics Data System (ADS)

Lee, Jaeyul; Shirazi, Muhammad Faizan; Park, Kibeom; Jeon, Mansik; Kim, Jeehyun

2017-12-01

Actuator lens industries have gained an enormous interest with the enhancement of various latest communication devices, such as mobile phone and notebooks. The quality of the aforementioned devices can be degraded due to the internal defects of actuator lenses. Therefore, in this study, we implemented swept-source optical coherence tomography (SS-OCT) system to inspect defects of actuator lenses. Owing to the high-resolution of the SS-OCT system, defected foreign substances between the actuator lenses, defective regions of lenses and surface stains were more clearly distinguished through three-dimensional (3D) and two-dimensional (2D) cross-sectional OCT images. Therefore, the implemented SS-OCT system can be considered as a potential application to defect inspection of actuator lens.

Wide-field optical mapping of neural activity and brain haemodynamics: considerations and novel approaches

PubMed Central

Ma, Ying; Shaik, Mohammed A.; Kozberg, Mariel G.; Thibodeaux, David N.; Zhao, Hanzhi T.; Yu, Hang

2016-01-01

Although modern techniques such as two-photon microscopy can now provide cellular-level three-dimensional imaging of the intact living brain, the speed and fields of view of these techniques remain limited. Conversely, two-dimensional wide-field optical mapping (WFOM), a simpler technique that uses a camera to observe large areas of the exposed cortex under visible light, can detect changes in both neural activity and haemodynamics at very high speeds. Although WFOM may not provide single-neuron or capillary-level resolution, it is an attractive and accessible approach to imaging large areas of the brain in awake, behaving mammals at speeds fast enough to observe widespread neural firing events, as well as their dynamic coupling to haemodynamics. Although such wide-field optical imaging techniques have a long history, the advent of genetically encoded fluorophores that can report neural activity with high sensitivity, as well as modern technologies such as light emitting diodes and sensitive and high-speed digital cameras have driven renewed interest in WFOM. To facilitate the wider adoption and standardization of WFOM approaches for neuroscience and neurovascular coupling research, we provide here an overview of the basic principles of WFOM, considerations for implementation of wide-field fluorescence imaging of neural activity, spectroscopic analysis and interpretation of results. This article is part of the themed issue ‘Interpreting BOLD: a dialogue between cognitive and cellular neuroscience’. PMID:27574312

Three-Dimensional Geometry of Collagenous Tissues by Second Harmonic Polarimetry.

PubMed

Reiser, Karen; Stoller, Patrick; Knoesen, André

2017-06-01

Collagen is a biological macromolecule capable of second harmonic generation, allowing label-free detection in tissues; in addition, molecular orientation can be determined from the polarization dependence of the second harmonic signal. Previously we reported that in-plane orientation of collagen fibrils could be determined by modulating the polarization angle of the laser during scanning. We have now extended this method so that out-of-plane orientation angles can be determined at the same time, allowing visualization of the 3-dimensional structure of collagenous tissues. This approach offers advantages compared with other methods for determining out-of-plane orientation. First, the orientation angles are directly calculated from the polarimetry data obtained in a single scan, while other reported methods require data from multiple scans, use of iterative optimization methods, application of fitting algorithms, or extensive post-optical processing. Second, our method does not require highly specialized instrumentation, and thus can be adapted for use in almost any nonlinear optical microscopy setup. It is suitable for both basic and clinical applications. We present three-dimensional images of structurally complex collagenous tissues that illustrate the power of such 3-dimensional analyses to reveal the architecture of biological structures.

Three-Dimensional Geometry of Collagenous Tissues by Second Harmonic Polarimetry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reiser, Karen; Stoller, Patrick; Knoesen, André

Collagen is a biological macromolecule capable of second harmonic generation, allowing label-free detection in tissues; in addition, molecular orientation can be determined from the polarization dependence of the second harmonic signal. Previously we reported that in-plane orientation of collagen fibrils could be determined by modulating the polarization angle of the laser during scanning. We have now extended this method so that out-of-plane orientation angles can be determined at the same time, allowing visualization of the 3-dimensional structure of collagenous tissues. This approach offers advantages compared with other methods for determining out-of-plane orientation. First, the orientation angles are directly calculated frommore » the polarimetry data obtained in a single scan, while other reported methods require data from multiple scans, use of iterative optimization methods, application of fitting algorithms, or extensive post-optical processing. Second, our method does not require highly specialized instrumentation, and thus can be adapted for use in almost any nonlinear optical microscopy setup. It is suitable for both basic and clinical applications. We present three-dimensional images of structurally complex collagenous tissues that illustrate the power of such 3-dimensional analyses to reveal the architecture of biological structures.« less

Three-Dimensional Geometry of Collagenous Tissues by Second Harmonic Polarimetry

DOE PAGES

Reiser, Karen; Stoller, Patrick; Knoesen, André

2017-06-01

Collagen is a biological macromolecule capable of second harmonic generation, allowing label-free detection in tissues; in addition, molecular orientation can be determined from the polarization dependence of the second harmonic signal. Previously we reported that in-plane orientation of collagen fibrils could be determined by modulating the polarization angle of the laser during scanning. We have now extended this method so that out-of-plane orientation angles can be determined at the same time, allowing visualization of the 3-dimensional structure of collagenous tissues. This approach offers advantages compared with other methods for determining out-of-plane orientation. First, the orientation angles are directly calculated frommore » the polarimetry data obtained in a single scan, while other reported methods require data from multiple scans, use of iterative optimization methods, application of fitting algorithms, or extensive post-optical processing. Second, our method does not require highly specialized instrumentation, and thus can be adapted for use in almost any nonlinear optical microscopy setup. It is suitable for both basic and clinical applications. We present three-dimensional images of structurally complex collagenous tissues that illustrate the power of such 3-dimensional analyses to reveal the architecture of biological structures.« less

Large-field-of-view wide-spectrum artificial reflecting superposition compound eyes

NASA Astrophysics Data System (ADS)

Huang, Chi-Chieh

The study of the imaging principles of natural compound eyes has become an active area of research and has fueled the advancement of modern optics with many attractive design features beyond those available with conventional technologies. Most prominent among all compound eyes is the reflecting superposition compound eyes (RSCEs) found in some decapods. They are extraordinary imaging systems with numerous optical features such as minimum chromatic aberration, wide-angle field of view (FOV), high sensitivity to light and superb acuity to motion. Inspired by their remarkable visual system, we were able to implement the unique lens-free, reflection-based imaging mechanisms into a miniaturized, large-FOV optical imaging device operating at the wide visible spectrum to minimize chromatic aberration without any additional post-image processing. First, two micro-transfer printing methods, a multiple and a shear-assisted transfer printing technique, were studied and discussed to realize life-sized artificial RSCEs. The processes exploited the differential adhesive tendencies of the microstructures formed between a donor and a transfer substrate to accomplish an efficient release and transfer process. These techniques enabled conformal wrapping of three-dimensional (3-D) microstructures, initially fabricated in two-dimensional (2-D) layouts with standard fabrication technology onto a wide range of surfaces with complex and curvilinear shapes. Final part of this dissertation was focused on implementing the key operational features of the natural RSCEs into large-FOV, wide-spectrum artificial RSCEs as an optical imaging device suitable for the wide visible spectrum. Our devices can form real, clear images based on reflection rather than refraction, hence avoiding chromatic aberration due to dispersion by the optical materials. Compared to the performance of conventional refractive lenses of comparable size, our devices demonstrated minimum chromatic aberration, exceptional FOV up to 165o without distortion, modest spherical aberrations and comparable imaging quality without any post-image processing. Together with an augmenting cruciform pattern surrounding each focused image, our devices possessed enhanced, dynamic motion-tracking capability ideal for diverse applications in military, security, search and rescue, night navigation, medical imaging and astronomy. In the future, due to its reflection-based operating principles, it can be further extended into mid- and far-infrared for more demanding applications.

Myocardial imaging using ultrahigh-resolution spectral domain optical coherence tomography

PubMed Central

Yao, Xinwen; Gan, Yu; Marboe, Charles C.; Hendon, Christine P.

2016-01-01

Abstract. We present an ultrahigh-resolution spectral domain optical coherence tomography (OCT) system in 800 nm with a low-noise supercontinuum source (SC) optimized for myocardial imaging. The system was demonstrated to have an axial resolution of 2.72  μm with a large imaging depth of 1.78 mm and a 6-dB falloff range of 0.89 mm. The lateral resolution (5.52  μm) was compromised to enhance the image penetration required for myocardial imaging. The noise of the SC source was analyzed extensively and an imaging protocol was proposed for SC-based OCT imaging with appreciable contrast. Three-dimensional datasets were acquired ex vivo on the endocardium side of tissue specimens from different chambers of fresh human and swine hearts. With the increased resolution and contrast, features such as elastic fibers, Purkinje fibers, and collagen fiber bundles were observed. The correlation between the structural information revealed in the OCT images and tissue pathology was discussed as well. PMID:27001162

Myocardial imaging using ultrahigh-resolution spectral domain optical coherence tomography

NASA Astrophysics Data System (ADS)

Yao, Xinwen; Gan, Yu; Marboe, Charles C.; Hendon, Christine P.

2016-06-01

We present an ultrahigh-resolution spectral domain optical coherence tomography (OCT) system in 800 nm with a low-noise supercontinuum source (SC) optimized for myocardial imaging. The system was demonstrated to have an axial resolution of 2.72 μm with a large imaging depth of 1.78 mm and a 6-dB falloff range of 0.89 mm. The lateral resolution (5.52 μm) was compromised to enhance the image penetration required for myocardial imaging. The noise of the SC source was analyzed extensively and an imaging protocol was proposed for SC-based OCT imaging with appreciable contrast. Three-dimensional datasets were acquired ex vivo on the endocardium side of tissue specimens from different chambers of fresh human and swine hearts. With the increased resolution and contrast, features such as elastic fibers, Purkinje fibers, and collagen fiber bundles were observed. The correlation between the structural information revealed in the OCT images and tissue pathology was discussed as well.

Tomographic and analog 3-D simulations using NORA. [Non-Overlapping Redundant Image Array formed by multiple pinholes

NASA Technical Reports Server (NTRS)

Yin, L. I.; Trombka, J. I.; Bielefeld, M. J.; Seltzer, S. M.

1984-01-01

The results of two computer simulations demonstrate the feasibility of using the nonoverlapping redundant array (NORA) to form three-dimensional images of objects with X-rays. Pinholes admit the X-rays to nonoverlapping points on a detector. The object is reconstructed in the analog mode by optical correlation and in the digital mode by tomographic computations. Trials were run with a stick-figure pyramid and extended objects with out-of-focus backgrounds. Substitution of spherical optical lenses for the pinholes increased the light transmission sufficiently that objects could be easily viewed in a dark room. Out-of-focus aberrations in tomographic reconstruction could be eliminated using Chang's (1976) algorithm.

Customized three-dimensional printed optical phantoms with user defined absorption and scattering

NASA Astrophysics Data System (ADS)

Pannem, Sanjana; Sweer, Jordan; Diep, Phuong; Lo, Justine; Snyder, Michael; Stueber, Gabriella; Zhao, Yanyu; Tabassum, Syeda; Istfan, Raeef; Wu, Junjie; Erramilli, Shyamsunder; Roblyer, Darren M.

2016-03-01

The use of reliable tissue-simulating phantoms spans multiple applications in spectroscopic imaging including device calibration and testing of new imaging procedures. Three-dimensional (3D) printing allows for the possibility of optical phantoms with arbitrary geometries and spatially varying optical properties. We recently demonstrated the ability to 3D print tissue-simulating phantoms with customized absorption (μa) and reduced scattering (μs`) by incorporating nigrosin, an absorbing dye, and titanium dioxide (TiO2), a scattering agent, to acrylonitrile butadiene styrene (ABS) during filament extrusion. A physiologically relevant range of μa and μs` was demonstrated with high repeatability. We expand our prior work here by evaluating the effect of two important 3D-printing parameters, percent infill and layer height, on both μa and μs`. 2 cm3 cubes were printed with percent infill ranging from 10% to 100% and layer height ranging from 0.15 to 0.40 mm. The range in μa and μs` was 27.3% and 19.5% respectively for different percent infills at 471 nm. For varying layer height, the range in μa and μs` was 27.8% and 15.4% respectively at 471 nm. These results indicate that percent infill and layer height substantially alter optical properties and should be carefully controlled during phantom fabrication. Through the use of inexpensive hobby-level printers, the fabrication of optical phantoms may advance the complexity and availability of fully customizable phantoms over multiple spatial scales. This technique exhibits a wider range of adaptability than other common methods of fabricating optical phantoms and may lead to improved instrument characterization and calibration.

Automated Geo/Co-Registration of Multi-Temporal Very-High-Resolution Imagery.

PubMed

Han, Youkyung; Oh, Jaehong

2018-05-17

For time-series analysis using very-high-resolution (VHR) multi-temporal satellite images, both accurate georegistration to the map coordinates and subpixel-level co-registration among the images should be conducted. However, applying well-known matching methods, such as scale-invariant feature transform and speeded up robust features for VHR multi-temporal images, has limitations. First, they cannot be used for matching an optical image to heterogeneous non-optical data for georegistration. Second, they produce a local misalignment induced by differences in acquisition conditions, such as acquisition platform stability, the sensor's off-nadir angle, and relief displacement of the considered scene. Therefore, this study addresses the problem by proposing an automated geo/co-registration framework for full-scene multi-temporal images acquired from a VHR optical satellite sensor. The proposed method comprises two primary steps: (1) a global georegistration process, followed by (2) a fine co-registration process. During the first step, two-dimensional multi-temporal satellite images are matched to three-dimensional topographic maps to assign the map coordinates. During the second step, a local analysis of registration noise pixels extracted between the multi-temporal images that have been mapped to the map coordinates is conducted to extract a large number of well-distributed corresponding points (CPs). The CPs are finally used to construct a non-rigid transformation function that enables minimization of the local misalignment existing among the images. Experiments conducted on five Kompsat-3 full scenes confirmed the effectiveness of the proposed framework, showing that the georegistration performance resulted in an approximately pixel-level accuracy for most of the scenes, and the co-registration performance further improved the results among all combinations of the georegistered Kompsat-3 image pairs by increasing the calculated cross-correlation values.

Active control of jet flowfields

NASA Astrophysics Data System (ADS)

Kibens, Valdis; Wlezien, Richard W.

1987-06-01

Passive and active control of jet shear layer development were investigated as mechanisms for modifying the global characteristics of jet flowfields. Slanted and stepped indeterminate origin (I.O.) nozzles were used as passive, geometry-based control devices which modified the flow origins. Active control techniques were also investigated, in which periodic acoustic excitation signals were injected into the I.O. nozzle shear layers. Flow visualization techniques based on a pulsed copper-vapor laser were used in a phase-conditioned image acquisition mode to assemble optically averaged sets of images acquired at known times throughout the repetition cycle of the basic flow oscillation period. Hot wire data were used to verify the effect of the control techniques on the mean and fluctuating flow properties. The flow visualization images were digitally enhanced and processed to show locations of prominent vorticity concentrations. Three-dimensional vortex interaction patterns were assembled in a format suitable for movie mode on a graphic display workstation, showing the evolution of three-dimensional vortex system in time.

High-Throughput Light Sheet Microscopy for the Automated Live Imaging of Larval Zebrafish

NASA Astrophysics Data System (ADS)

Baker, Ryan; Logan, Savannah; Dudley, Christopher; Parthasarathy, Raghuveer

The zebrafish is a model organism with a variety of useful properties; it is small and optically transparent, it reproduces quickly, it is a vertebrate, and there are a large variety of transgenic animals available. Because of these properties, the zebrafish is well suited to study using a variety of optical technologies including light sheet fluorescence microscopy (LSFM), which provides high-resolution three-dimensional imaging over large fields of view. Research progress, however, is often not limited by optical techniques but instead by the number of samples one can examine over the course of an experiment, which in the case of light sheet imaging has so far been severely limited. Here we present an integrated fluidic circuit and microscope which provides rapid, automated imaging of zebrafish using several imaging modes, including LSFM, Hyperspectral Imaging, and Differential Interference Contrast Microscopy. Using this system, we show that we can increase our imaging throughput by a factor of 10 compared to previous techniques. We also show preliminary results visualizing zebrafish immune response, which is sensitive to gut microbiota composition, and which shows a strong variability between individuals that highlights the utility of high throughput imaging. National Science Foundation, Award No. DBI-1427957.

Three-dimensional anterior segment imaging in patients with type 1 Boston Keratoprosthesis with switchable full depth range swept source optical coherence tomography

PubMed Central

Poddar, Raju; Cortés, Dennis E.; Werner, John S.; Mannis, Mark J.

2013-01-01

Abstract. A high-speed (100 kHz A-scans/s) complex conjugate resolved 1 μm swept source optical coherence tomography (SS-OCT) system using coherence revival of the light source is suitable for dense three-dimensional (3-D) imaging of the anterior segment. The short acquisition time helps to minimize the influence of motion artifacts. The extended depth range of the SS-OCT system allows topographic analysis of clinically relevant images of the entire depth of the anterior segment of the eye. Patients with the type 1 Boston Keratoprosthesis (KPro) require evaluation of the full anterior segment depth. Current commercially available OCT systems are not suitable for this application due to limited acquisition speed, resolution, and axial imaging range. Moreover, most commonly used research grade and some clinical OCT systems implement a commercially available SS (Axsun) that offers only 3.7 mm imaging range (in air) in its standard configuration. We describe implementation of a common swept laser with built-in k-clock to allow phase stable imaging in both low range and high range, 3.7 and 11.5 mm in air, respectively, without the need to build an external MZI k-clock. As a result, 3-D morphology of the KPro position with respect to the surrounding tissue could be investigated in vivo both at high resolution and with large depth range to achieve noninvasive and precise evaluation of success of the surgical procedure. PMID:23912759

Magnetomotive imaging of iron oxide nanoparticles as cellular contrast agents for optical coherence tomography

NASA Astrophysics Data System (ADS)

Cimalla, Peter; Werner, Theresa; Gaertner, Maria; Mueller, Claudia; Walther, Julia; Wittig, Dierk; Ader, Marius; Karl, Mike; Koch, Edmund

2013-06-01

Recent studies in animal models provided proof-of-principle evidence for cell transplantation as a potential future therapeutic approach for retinal pathologies in humans such as Retinitis pigmentosa or age-related macular degeneration. In this case, donor cells are injected into the eye in order to protect or replace degenerating photoreceptors or retinal pigment epithelium. However, currently there is no three-dimensional imaging technique available that allows tracking of cell migration and integration into the host tissue under in vivo conditions. Therefore, we investigate about magnetomotive optical coherence tomography (OCT) of substances labeled with iron oxide nanoparticles as a potential method for noninvasive, three-dimensional cell tracking in the retina. We use a self-developed spectral domain OCT system for high-resolution imaging in the 800 nm-wavelength region. A suitable AC magnetic field for magnetomotive imaging was generated using two different setups, which consist of an electrically driven solenoid in combination with a permanent magnet, and a mechanically driven all-permanent magnet configuration. In the sample region the maximum magnetic flux density was 100 mT for both setups, with a field gradient of 9 T/m and 13 T/m for the solenoid and the allpermanent magnet setup, respectively. Magnetomotive OCT imaging was performed in elastic tissue phantoms and single cells labeled with iron oxide nanoparticles. Particle-induced sub-resolution movement of the elastic samples and the single cells could successfully be detected and visualized by means of phase-resolved Doppler OCT analysis. Therefore, this method is a potential technique to enhance image contrast of specific cells in OCT.

Imaging with Second-Harmonic Generation Nanoparticles

NASA Astrophysics Data System (ADS)

Hsieh, Chia-Lung

Second-harmonic generation nanoparticles show promise as imaging probes due to their coherent and stable signal with a broad flexibility in the choice of excitation wavelength. In this thesis, we developed and demonstrated barium titanate nanoparticles as second-harmonic radiation imaging probes. We studied the absolute second-harmonic generation efficiency of the nanoparticles on single-particle level. The polarization dependent second-harmonic signal of single nanoparticles was studied in detail. From the measured polar response, we were able to find the orientation of the nanoparticle. We developed a biochemical interface for using the second-harmonic nanoprobes as biomarkers, including in vitro cellular imaging and in vivo live animal imaging. The nanoparticles were surface functionalized with primary amine groups for stable colloidal dispersion. We achieved specific labeling of the second-harmonic nanoprobes via immunostaining where the antibodies were covalently conjugated onto the nanoparticles. We observed no toxicity of the functionalized nanoparticles to biological cells. The coherent second-harmonic signal radiated from the nanoparticles offers opportunities for new imaging techniques. Using interferometric detection, namely harmonic holography, both amplitude and phase of the second-harmonic field can be captured. Through digital beam propagation, three-dimensional field distribution, reflecting three-dimensional distribution of the nanoparticles, can be reconstructed. We achieved a scan-free three-dimensional imaging of nanoparticles in biological cells with sub-micron spatial resolution by using the harmonic holographic microscope. We further exploited the coherent second-harmonic signal for imaging through scattering media by performing optical phase conjugation of the second-harmonic signal. We demonstrated an all-digital optical phase conjugation of the second-harmonic signal originated from a nanoparticle by combining harmonic holography and dynamic computer generated holography using a spatial light modulator. The phase-conjugated second-harmonic scattered field retraced the scattering trajectory and formed a clean focus on the nanoparticle placed inside a scattering medium. The nanoparticle acted as a beacon of light; it helped us find the tailored wavefront for concentrating light at the nanoparticle inside the scattering medium. We also demonstrated imaging through a thin scattering medium by raster-scanning the phase-conjugated focus in the vicinity of the beacon nanoparticle, in which a clear image of a target placed behind a ground glass diffuser was obtained.

Speckle contrast optical tomography: A new method for deep tissue three-dimensional tomography of blood flow

PubMed Central

Varma, Hari M.; Valdes, Claudia P.; Kristoffersen, Anna K.; Culver, Joseph P.; Durduran, Turgut

2014-01-01

A novel tomographic method based on the laser speckle contrast, speckle contrast optical tomography (SCOT) is introduced that allows us to reconstruct three dimensional distribution of blood flow in deep tissues. This method is analogous to the diffuse optical tomography (DOT) but for deep tissue blood flow. We develop a reconstruction algorithm based on first Born approximation to generate three dimensional distribution of flow using the experimental data obtained from tissue simulating phantoms. PMID:24761306

Nonuniform multiview color texture mapping of image sequence and three-dimensional model for faded cultural relics with sift feature points

NASA Astrophysics Data System (ADS)

Li, Na; Gong, Xingyu; Li, Hongan; Jia, Pengtao

2018-01-01

For faded relics, such as Terracotta Army, the 2D-3D registration between an optical camera and point cloud model is an important part for color texture reconstruction and further applications. This paper proposes a nonuniform multiview color texture mapping for the image sequence and the three-dimensional (3D) model of point cloud collected by Handyscan3D. We first introduce nonuniform multiview calibration, including the explanation of its algorithm principle and the analysis of its advantages. We then establish transformation equations based on sift feature points for the multiview image sequence. At the same time, the selection of nonuniform multiview sift feature points is introduced in detail. Finally, the solving process of the collinear equations based on multiview perspective projection is given with three steps and the flowchart. In the experiment, this method is applied to the color reconstruction of the kneeling figurine, Tangsancai lady, and general figurine. These results demonstrate that the proposed method provides an effective support for the color reconstruction of the faded cultural relics and be able to improve the accuracy of 2D-3D registration between the image sequence and the point cloud model.

WE-D-17A-02: Evaluation of a Two-Dimensional Optical Dosimeter On Measuring Lateral Profiles of Proton Pencil Beams

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hsi, W; Lee, T; Schultz, T

Purpose: To evaluate the accuracy of a two-dimensional optical dosimeter on measuring lateral profiles for spots and scanned fields of proton pencil beams. Methods: A digital camera with a color image senor was utilized to image proton-induced scintillations on Gadolinium-oxysulfide phosphor reflected by a stainless-steel mirror. Intensities of three colors were summed for each pixel with proper spatial-resolution calibration. To benchmark this dosimeter, the field size and penumbra for 100mm square fields of singleenergy pencil-scan protons were measured and compared between this optical dosimeter and an ionization-chamber profiler. Sigma widths of proton spots in air were measured and compared betweenmore » this dosimeter and a commercial optical dosimeter. Clinical proton beams with ranges between 80 mm and 300 mm at CDH proton center were used for this benchmark. Results: Pixel resolutions vary 1.5% between two perpendicular axes. For a pencil-scan field with 302 mm range, measured field sizes and penumbras between two detection systems agreed to 0.5 mm and 0.3 mm, respectively. Sigma widths agree to 0.3 mm between two optical dosimeters for a proton spot with 158 mm range; having widths of 5.76 mm and 5.92 mm for X and Y axes, respectively. Similar agreements were obtained for others beam ranges. This dosimeter was successfully utilizing on mapping the shapes and sizes of proton spots at the technical acceptance of McLaren proton therapy system. Snow-flake spots seen on images indicated the image sensor having pixels damaged by radiations. Minor variations in intensity between different colors were observed. Conclusions: The accuracy of our dosimeter was in good agreement with other established devices in measuring lateral profiles of pencil-scan fields and proton spots. A precise docking mechanism for camera was designed to keep aligned optical path while replacing damaged image senor. Causes for minor variations between emitted color lights will be investigated.« less

Light field creating and imaging with different order intensity derivatives

NASA Astrophysics Data System (ADS)

Wang, Yu; Jiang, Huan

2014-10-01

Microscopic image restoration and reconstruction is a challenging topic in the image processing and computer vision, which can be widely applied to life science, biology and medicine etc. A microscopic light field creating and three dimensional (3D) reconstruction method is proposed for transparent or partially transparent microscopic samples, which is based on the Taylor expansion theorem and polynomial fitting. Firstly the image stack of the specimen is divided into several groups in an overlapping or non-overlapping way along the optical axis, and the first image of every group is regarded as reference image. Then different order intensity derivatives are calculated using all the images of every group and polynomial fitting method based on the assumption that the structure of the specimen contained by the image stack in a small range along the optical axis are possessed of smooth and linear property. Subsequently, new images located any position from which to reference image the distance is Δz along the optical axis can be generated by means of Taylor expansion theorem and the calculated different order intensity derivatives. Finally, the microscopic specimen can be reconstructed in 3D form using deconvolution technology and all the images including both the observed images and the generated images. The experimental results show the effectiveness and feasibility of our method.

Four-Dimensional Screening Anti-Counterfeiting Pattern by Inkjet Printed Photonic Crystals.

PubMed

Hou, Jue; Zhang, Huacheng; Su, Bin; Li, Mingzhu; Yang, Qiang; Jiang, Lei; Song, Yanlin

2016-10-06

A four-dimensional screening anti-counterfeiting QR code composed of differently shaped photonic crystal (PC) dots has been fabricated that could display four images depending on different lighting conditions. By controlling the rheology of poly(dimethylsiloxane) (PDMS), three kinds of PC dots could be sequentially integrated into one pattern using the layer-by-layer printing strategy. The information can be encoded and stored in shapes and read out by the difference in optical properties. © 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

A compact structured light based otoscope for three dimensional imaging of the tympanic membrane

NASA Astrophysics Data System (ADS)

Das, Anshuman J.; Estrada, Julio C.; Ge, Zhifei; Dolcetti, Sara; Chen, Deborah; Raskar, Ramesh

2015-02-01

Three dimensional (3D) imaging of the tympanic membrane (TM) has been carried out using a traditional otoscope equipped with a high-definition webcam, a portable projector and a telecentric optical system. The device allows us to project fringe patterns on the TM and the magnified image is processed using phase shifting algorithms to arrive at a 3D description of the TM. Obtaining a 3D image of the TM can aid in the diagnosis of ear infections such as otitis media with effusion, which is essentially fluid build-up in the middle ear. The high resolution of this device makes it possible examine a computer generated 3D profile for abnormalities in the shape of the eardrum. This adds an additional dimension to the image that can be obtained from a traditional otoscope by allowing visualization of the TM from different perspectives. In this paper, we present the design and construction of this device and details of the imaging processing for recovering the 3D profile of the subject under test. The design of the otoscope is similar to that of the traditional device making it ergonomically compatible and easy to adopt in clinical practice.

In vivo multi-modality photoacoustic and pulse echo tracking of prostate tumor growth using a window chamber

NASA Astrophysics Data System (ADS)

Bauer, Daniel R.; Olafsson, Ragnar; Montilla, Leonardo G.; Witte, Russell S.

2010-02-01

Understanding the tumor microenvironment is critical to characterizing how cancers operate and predicting how they will eventually respond to treatment. The mouse window chamber model is an excellent tool for cancer research, because it enables high resolution tumor imaging and cross-validation using multiple modalities. We describe a novel multimodality imaging system that incorporates three dimensional (3D) photoacoustics with pulse echo ultrasound for imaging the tumor microenvironment and tracking tissue growth in mice. Three mice were implanted with a dorsal skin flap window chamber. PC-3 prostate tumor cells, expressing green fluorescent protein (GFP), were injected into the skin. The ensuing tumor invasion was mapped using photoacoustic and pulse echo imaging, as well as optical and fluorescent imaging for comparison and cross validation. The photoacoustic imaging and spectroscopy system, consisting of a tunable (680-1000nm) pulsed laser and 25 MHz ultrasound transducer, revealed near infrared absorbing regions, primarily blood vessels. Pulse echo images, obtained simultaneously, provided details of the tumor microstructure and growth with 100-μm3 resolution. The tumor size in all three mice increased between three and five fold during 3+ weeks of imaging. Results were consistent with the optical and fluorescent images. Photoacoustic imaging revealed detailed maps of the tumor vasculature, whereas photoacoustic spectroscopy identified regions of oxygenated and deoxygenated blood vessels. The 3D photoacoustic and pulse echo imaging system provided complementary information to track the tumor microenvironment, evaluate new cancer therapies, and develop molecular imaging agents in vivo. Finally, these safe and noninvasive techniques are potentially applicable for human cancer imaging.

Dual-detection confocal fluorescence microscopy: fluorescence axial imaging without axial scanning.

PubMed

Lee, Dong-Ryoung; Kim, Young-Duk; Gweon, Dae-Gab; Yoo, Hongki

2013-07-29

We propose a new method for high-speed, three-dimensional (3-D) fluorescence imaging, which we refer to as dual-detection confocal fluorescence microscopy (DDCFM). In contrast to conventional beam-scanning confocal fluorescence microscopy, where the focal spot must be scanned either optically or mechanically over a sample volume to reconstruct a 3-D image, DDCFM can obtain the depth of a fluorescent emitter without depth scanning. DDCFM comprises two photodetectors, each with a pinhole of different size, in the confocal detection system. Axial information on fluorescent emitters can be measured by the axial response curve through the ratio of intensity signals. DDCFM can rapidly acquire a 3-D fluorescent image from a single two-dimensional scan with less phototoxicity and photobleaching than confocal fluorescence microscopy because no mechanical depth scans are needed. We demonstrated the feasibility of the proposed method by phantom studies.

Experimental investigation on the caries characteristic of dental tissues by photothermal radiometry scanning imaging

NASA Astrophysics Data System (ADS)

Wang, Fei; Liu, Jun-yan; Wang, Xiao-chun; Wang, Yang

2018-03-01

In this paper, a one-dimensional (1D) thermal-wave model coupled diffuse-photon-density-wave for three-layer dental tissues using modulated laser stimulation was employed to illustrate the relationship between dental caries characteristic (i.e. caries layer thickness, optical absorption coefficient and optical scattering coefficient) and photothermal radiometry (PTR) signal. Experimental investigation of artificial caries was carried out using PTR scanning imaging. The PTR amplitude and phase delay were increased with dental demineralized treatment. The local caries characteristic parameters were obtained by the best-fitting method based on the 1D thermal-wave model. The PTR scanning imaging measurements illustrated that the optical absorption coefficient and scattering coefficient of caries region were much higher than those of the healthy enamel area. The demineralization thickness of caries region was measured by PTR scanning imaging and its average value shows in good agreement with the digital microscope. Experimental results show that PTR scanning imaging has the merits of high contrast for local inhomogeneity of dental caries; furthermore, this method is an allowance to provide a flexibility for non-contact quantitative evaluation of dental caries.

Optical scanning holography based on compressive sensing using a digital micro-mirror device

NASA Astrophysics Data System (ADS)

A-qian, Sun; Ding-fu, Zhou; Sheng, Yuan; You-jun, Hu; Peng, Zhang; Jian-ming, Yue; xin, Zhou

2017-02-01

Optical scanning holography (OSH) is a distinct digital holography technique, which uses a single two-dimensional (2D) scanning process to record the hologram of a three-dimensional (3D) object. Usually, these 2D scanning processes are in the form of mechanical scanning, and the quality of recorded hologram may be affected due to the limitation of mechanical scanning accuracy and unavoidable vibration of stepper motor's start-stop. In this paper, we propose a new framework, which replaces the 2D mechanical scanning mirrors with a Digital Micro-mirror Device (DMD) to modulate the scanning light field, and we call it OSH based on Compressive Sensing (CS) using a digital micro-mirror device (CS-OSH). CS-OSH can reconstruct the hologram of an object through the use of compressive sensing theory, and then restore the image of object itself. Numerical simulation results confirm this new type OSH can get a reconstructed image with favorable visual quality even under the condition of a low sample rate.

Lasing in optimized two-dimensional iron-nail-shaped rod photonic crystals

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kwon, Soon-Yong; Moon, Seul-Ki; Yang, Jin-Kyu, E-mail: jinkyuyang@kongju.ac.kr

2016-03-15

We demonstrated lasing at the Γ-point band-edge (BE) modes in optimized two-dimensional iron-nail-shaped rod photonic crystals by optical pulse pumping at room temperature. As the radius of the rod increased quadratically toward the edge of the pattern, the quality factor of the Γ-point BE mode increased up to three times, and the modal volume decreased to 56% compared with the values of the original Γ-point BE mode because of the reduction of the optical loss in the horizontal direction. Single-mode lasing from an optimized iron-nail-shaped rod array with an InGaAsP multiple quantum well embedded in the nail heads was observedmore » at a low threshold pump power of 160 μW. Real-image-based numerical simulations showed that the lasing actions originated from the optimized Γ-point BE mode and agreed well with the measurement results, including the lasing polarization, wavelength, and near-field image.« less

Spray pattern analysis in TWAS using photogrammetry and digital image correlation

NASA Astrophysics Data System (ADS)

Tillmann, W.; Rademacher, H. G.; Hagen, L.; Abdulgader, M.; El Barad’ei, M.

2018-06-01

In terms of arc spraying processes, the spray plume characteristic is mainly affected by the flow characteristic of the atomization gas at the nozzle inlet and intersection point of the wire tips, which in turn affect the particle distribution at the moment of impact when molten spray particles splash onto the substrate. With respect to the route of manufacturing of near net-shaped coatings on complex geometries, the acquisition of the spray patterns is pressingly necessary to determine the produced coating thickness. Within the scope of this study, computer fluid dynamics (CFD) simulations were carried out to determine the distribution of spray particles for different spray parameter settings. The results were evaluated by three-dimensional spray spot analyses using an optical measurement based on photogrammetry and digital image correlation. The optical measurement represents a promising and much faster candidate to measure spray patterns compared to the tactile measurement system but with an equal accuracy. For given nozzle configurations and spray parameter settings, numerous spray patterns were examined to their shape factors, demonstrating the potential of an online analysis, which encompasses a “fast sample loop” and a data processing system to generate a three-dimensional surface of the spray spot profile.

HOMER: the Holographic Optical Microscope for Education and Research

NASA Astrophysics Data System (ADS)

Luviano, Anali

Holography was invented in 1948 by Dennis Gabor and has undergone major advancements since the 2000s leading to the development of commercial digital holographic microscopes (DHM). This noninvasive form of microscopy produces a three-dimensional (3-D) digital model of a sample without altering or destroying the sample, thus allowing the same sample to be studied multiple times. HOMER-the Holographic Optical Microscope for Education and Research-produces a 3-D image from a two-dimensional (2-D) interference pattern captured by a camera that is then put through reconstruction software. This 2-D pattern is created when a reference wave interacts with the sample to produce a secondary wave that interferes with the unaltered part of the reference wave. I constructed HOMER to be an efficient, portable in-line DHM using inexpensive material and free reconstruction software. HOMER uses three different-colored LEDs as light sources. I am testing the performance of HOMER with the goal of producing tri-color images of samples. I'm using small basic biological samples to test the effectiveness of HOMER and plan to transition to complex cellular and biological specimens as I pursue my interest in biophysics. Norwich University.

Imaging of cardiovascular structures using near-infrared femtosecond multiphoton laser scanning microscopy.

PubMed

Schenke-Layland, Katja; Riemann, Iris; Stock, Ulrich A; König, Karsten

2005-01-01

Multiphoton imaging represents a novel and very promising medical diagnostic technology for the high-resolution analysis of living biological tissues. We performed multiphoton imaging to analyzed structural features of extracellular matrix (ECM) components, e.g., collagen and elastin, of vital pulmonary and aortic heart valves. High-resolution autofluorescence images of collagenous and elastic fibers were demonstrated using multifluorophore, multiphoton excitation at two different wavelengths and optical sectioning, without the requirement of embedding, fixation, or staining. Collagenous structures were selectively imaged by detection of second harmonic generation (SHG). Additionally, routine histology and electron microscopy were integrated to verify the observed results. In comparison with pulmonary tissues, aortic heart valve specimens show very similar matrix formations. The quality of the resulting three-dimensional (3-D) images enabled the differentiation between collagenous and elastic fibers. These experimental results indicate that multiphoton imaging with near-infrared (NIR) femtosecond laser pulses may prove to be a useful tool for the nondestructive monitoring and characterization of cardiovascular structures. Copyright 2005 Society of Photo-Optical Instrumentation Engineers.

A method for three-dimensional quantitative observation of the microstructure of biological samples

NASA Astrophysics Data System (ADS)

Wang, Pengfei; Chen, Dieyan; Ma, Wanyun; Wu, Hongxin; Ji, Liang; Sun, Jialin; Lv, Danyu; Zhang, Lu; Li, Ying; Tian, Ning; Zheng, Jinggao; Zhao, Fengying

2009-07-01

Contemporary biology has developed into the era of cell biology and molecular biology, and people try to study the mechanism of all kinds of biological phenomena at the microcosmic level now. Accurate description of the microstructure of biological samples is exigent need from many biomedical experiments. This paper introduces a method for 3-dimensional quantitative observation on the microstructure of vital biological samples based on two photon laser scanning microscopy (TPLSM). TPLSM is a novel kind of fluorescence microscopy, which has excellence in its low optical damage, high resolution, deep penetration depth and suitability for 3-dimensional (3D) imaging. Fluorescent stained samples were observed by TPLSM, and afterward the original shapes of them were obtained through 3D image reconstruction. The spatial distribution of all objects in samples as well as their volumes could be derived by image segmentation and mathematic calculation. Thus the 3-dimensionally and quantitatively depicted microstructure of the samples was finally derived. We applied this method to quantitative analysis of the spatial distribution of chromosomes in meiotic mouse oocytes at metaphase, and wonderful results came out last.

Rationalisation and Validation of an Acrylamide-Free Procedure in Three-Dimensional Histological Imaging

PubMed Central

Lai, Hei Ming; Liu, Alan King Lun; Ng, Wai-Lung; DeFelice, John; Lee, Wing Sang; Li, Heng; Li, Wen; Ng, Ho Man; Chang, Raymond Chuen-Chung; Lin, Bin; Wu, Wutian; Gentleman, Steve M.

2016-01-01

Three-dimensional visualization of intact tissues is now being achieved by turning tissues transparent. CLARITY is a unique tissue clearing technique, which features the use of detergents to remove lipids from fixed tissues to achieve optical transparency. To preserve tissue integrity, an acrylamide-based hydrogel has been proposed to embed the tissue. In this study, we examined the rationale behind the use of acrylamide in CLARITY, and presented evidence to suggest that the omission of acrylamide-hydrogel embedding in CLARITY does not alter the preservation of tissue morphology and molecular information in fixed tissues. We therefore propose a novel and simplified workflow for formaldehyde-fixed tissue clearing, which will facilitate the laboratory implementation of this technique. Furthermore, we have investigated the basic tissue clearing process in detail and have highlighted some areas for targeted improvement of technologies essential for the emerging subject of three-dimensional histology. PMID:27359336

Optical metabolic imaging of colorectal adenocarcinoma derived organoids: assessing cellular-level resistance to therapy (Conference Presentation)

NASA Astrophysics Data System (ADS)

James, Haley M.; Prieto, Sandra P.; Greening, Gage J.; Muldoon, Timothy J.

2017-02-01

Locally advanced adenocarcinomas located in the distal rectum are commonly treated via 5-fluorouracil (5-FU)-based neoadjuvant chemoradiation therapy (CRT). The occurrence of pre-operative pathological complete response, or the absence of any histological evidence of residual cancer, is seen in 15-27% of rectal cancer cases. Response to chemotherapeutic agents varies between patients, introducing the need for a system to predict optimal drug combinations. We propose a method of utilizing optical metabolic imaging of in vitro, primary tumor-derived, three-dimensional organoid culture to create specific drug sensitivity profiles, and to rapidly assess a patient's potential response to drugs. Murine xenografts were developed in Swiss athymic nude mice, using human colorectal adenocarcinoma cell lines, implanted in the flank (RKO, ATCC). Tumors were excised upon reaching a volume of 500mm3 and processed for organoid culture. Organoids were subjected to longitudinal metabolic imaging of metabolic cofactors FAD and NADH for seven days. The resulting images were used to yield an optical redox value on a cell-by-cell basis, determined by the fluorescence intensity ratio of FAD/(FAD+NADH). This data infers proliferative index of the organoids. Beginning on day three, a control vehicle dimethyl sulfoxide, or the cytotoxic agent 5-FU, was added to the organoid growth media in wells, with metabolic imaging performed the same as previously stated. The optical redox values decreased due to the addition of 5-FU, which targets rapidly dividing cells and induces apoptosis. The changes in the optical redox histograms were correlated to markers of cell proliferation (Ki-67) and apoptosis (cleaved caspase-3).

Multiview three-dimensional display with continuous motion parallax through planar aligned OLED microdisplays.

PubMed

Teng, Dongdong; Xiong, Yi; Liu, Lilin; Wang, Biao

2015-03-09

Existing multiview three-dimensional (3D) display technologies encounter discontinuous motion parallax problem, due to a limited number of stereo-images which are presented to corresponding sub-viewing zones (SVZs). This paper proposes a novel multiview 3D display system to obtain continuous motion parallax by using a group of planar aligned OLED microdisplays. Through blocking partial light-rays by baffles inserted between adjacent OLED microdisplays, transitional stereo-image assembled by two spatially complementary segments from adjacent stereo-images is presented to a complementary fusing zone (CFZ) which locates between two adjacent SVZs. For a moving observation point, the spatial ratio of the two complementary segments evolves gradually, resulting in continuously changing transitional stereo-images and thus overcoming the problem of discontinuous motion parallax. The proposed display system employs projection-type architecture, taking the merit of full display resolution, but at the same time having a thin optical structure, offering great potentials for portable or mobile 3D display applications. Experimentally, a prototype display system is demonstrated by 9 OLED microdisplays.

Transparent 3D display for augmented reality

NASA Astrophysics Data System (ADS)

Lee, Byoungho; Hong, Jisoo

2012-11-01

Two types of transparent three-dimensional display systems applicable for the augmented reality are demonstrated. One of them is a head-mounted-display-type implementation which utilizes the principle of the system adopting the concave floating lens to the virtual mode integral imaging. Such configuration has an advantage in that the threedimensional image can be displayed at sufficiently far distance resolving the accommodation conflict with the real world scene. Incorporating the convex half mirror, which shows a partial transparency, instead of the concave floating lens, makes it possible to implement the transparent three-dimensional display system. The other type is the projection-type implementation, which is more appropriate for the general use than the head-mounted-display-type implementation. Its imaging principle is based on the well-known reflection-type integral imaging. We realize the feature of transparent display by imposing the partial transparency to the array of concave mirror which is used for the screen of reflection-type integral imaging. Two types of configurations, relying on incoherent and coherent light sources, are both possible. For the incoherent configuration, we introduce the concave half mirror array, whereas the coherent one adopts the holographic optical element which replicates the functionality of the lenslet array. Though the projection-type implementation is beneficial than the head-mounted-display in principle, the present status of the technical advance of the spatial light modulator still does not provide the satisfactory visual quality of the displayed three-dimensional image. Hence we expect that the head-mounted-display-type and projection-type implementations will come up in the market in sequence.

Hyperspectral and multispectral bioluminescence optical tomography for small animal imaging.

PubMed

Chaudhari, Abhijit J; Darvas, Felix; Bading, James R; Moats, Rex A; Conti, Peter S; Smith, Desmond J; Cherry, Simon R; Leahy, Richard M

2005-12-07

For bioluminescence imaging studies in small animals, it is important to be able to accurately localize the three-dimensional (3D) distribution of the underlying bioluminescent source. The spectrum of light produced by the source that escapes the subject varies with the depth of the emission source because of the wavelength-dependence of the optical properties of tissue. Consequently, multispectral or hyperspectral data acquisition should help in the 3D localization of deep sources. In this paper, we describe a framework for fully 3D bioluminescence tomographic image acquisition and reconstruction that exploits spectral information. We describe regularized tomographic reconstruction techniques that use semi-infinite slab or FEM-based diffusion approximations of photon transport through turbid media. Singular value decomposition analysis was used for data dimensionality reduction and to illustrate the advantage of using hyperspectral rather than achromatic data. Simulation studies in an atlas-mouse geometry indicated that sub-millimeter resolution may be attainable given accurate knowledge of the optical properties of the animal. A fixed arrangement of mirrors and a single CCD camera were used for simultaneous acquisition of multispectral imaging data over most of the surface of the animal. Phantom studies conducted using this system demonstrated our ability to accurately localize deep point-like sources and show that a resolution of 1.5 to 2.2 mm for depths up to 6 mm can be achieved. We also include an in vivo study of a mouse with a brain tumour expressing firefly luciferase. Co-registration of the reconstructed 3D bioluminescent image with magnetic resonance images indicated good anatomical localization of the tumour.

Noncoherent parallel optical processor for discrete two-dimensional linear transformations.

PubMed

Glaser, I

1980-10-01

We describe a parallel optical processor, based on a lenslet array, that provides general linear two-dimensional transformations using noncoherent light. Such a processor could become useful in image- and signal-processing applications in which the throughput requirements cannot be adequately satisfied by state-of-the-art digital processors. Experimental results that illustrate the feasibility of the processor by demonstrating its use in parallel optical computation of the two-dimensional Walsh-Hadamard transformation are presented.

Docking alignment system

NASA Technical Reports Server (NTRS)

Monford, Leo G. (Inventor)

1990-01-01

Improved techniques are provided for alignment of two objects. The present invention is particularly suited for three-dimensional translation and three-dimensional rotational alignment of objects in outer space. A camera 18 is fixedly mounted to one object, such as a remote manipulator arm 10 of the spacecraft, while the planar reflective surface 30 is fixed to the other object, such as a grapple fixture 20. A monitor 50 displays in real-time images from the camera, such that the monitor displays both the reflected image of the camera and visible markings on the planar reflective surface when the objects are in proper alignment. The monitor may thus be viewed by the operator and the arm 10 manipulated so that the reflective surface is perpendicular to the optical axis of the camera, the roll of the reflective surface is at a selected angle with respect to the camera, and the camera is spaced a pre-selected distance from the reflective surface.

Preparation strategy and illumination of three-dimensional cell cultures in light sheet-based fluorescence microscopy

NASA Astrophysics Data System (ADS)

Bruns, Thomas; Schickinger, Sarah; Wittig, Rainer; Schneckenburger, Herbert

2012-10-01

A device for selective plane illumination microscopy (SPIM) of three-dimensional multicellular spheroids, in culture medium under stationary or microfluidic conditions, is described. Cell spheroids are located in a micro-capillary and a light sheet, for illumination, is generated in an optical setup adapted to a conventional inverse microscope. Layers of the sample, of about 10 μm or less in diameter, are, thus, illuminated selectively and imaged by high resolution fluorescence microscopy. SPIM is operated at low light exposure even if a larger number of layers is imaged and is easily combined with laser scanning microscopy. Chinese hamster ovary cells expressing a membrane-associated green fluorescent protein are used for preliminary tests, and the uptake of the fluorescent marker, acridine orange via a microfluidic system, is visualized to demonstrate its potential in cancer research such as for the detection of cellular responses to anticancer drugs.

Tomographic techniques for the study of exceptionally preserved fossils

PubMed Central

Sutton, Mark D

2008-01-01

Three-dimensional fossils, especially those preserving soft-part anatomy, are a rich source of palaeontological information; they can, however, be difficult to work with. Imaging of serial planes through an object (tomography) allows study of both the inside and outside of three-dimensional fossils. Tomography may be performed using physical grinding or sawing coupled with photography, through optical techniques of serial focusing, or using a variety of scanning technologies such as neutron tomography, magnetic resonance imaging and most usefully X-ray computed tomography. This latter technique is applicable at a variety of scales, and when combined with a synchrotron X-ray source can produce very high-quality data that may be augmented by phase-contrast information to enhance contrast. Tomographic data can be visualized in several ways, the most effective of which is the production of isosurface-based ‘virtual fossils’ that can be manipulated and dissected interactively. PMID:18426749

Multi-pixel high-resolution three-dimensional imaging radar

NASA Technical Reports Server (NTRS)

Cooper, Ken B. (Inventor); Dengler, Robert J. (Inventor); Siegel, Peter H. (Inventor); Chattopadhyay, Goutam (Inventor); Ward, John S. (Inventor); Juan, Nuria Llombart (Inventor); Bryllert, Tomas E. (Inventor); Mehdi, Imran (Inventor); Tarsala, Jan A. (Inventor)

2012-01-01

A three-dimensional imaging radar operating at high frequency e.g., 670 GHz radar using low phase-noise synthesizers and a fast chirper to generate a frequency-modulated continuous-wave (FMCW) waveform, is disclosed that operates with a multiplexed beam to obtain range information simultaneously on multiple pixels of a target. A source transmit beam may be divided by a hybrid coupler into multiple transmit beams multiplexed together and directed to be reflected off a target and return as a single receive beam which is demultiplexed and processed to reveal range information of separate pixels of the target associated with each transmit beam simultaneously. The multiple transmit beams may be developed with appropriate optics to be temporally and spatially differentiated before being directed to the target. Temporal differentiation corresponds to a different intermediate frequencies separating the range information of the multiple pixels. Collinear transmit beams having differentiated polarizations may also be implemented.

A three-dimensional stereotaxic MRI brain atlas of the cichlid fish Oreochromis mossambicus.

PubMed

Simões, José M; Teles, Magda C; Oliveira, Rui F; Van der Linden, Annemie; Verhoye, Marleen

2012-01-01

The African cichlid Oreochromis mossambicus (Mozambique tilapia) has been used as a model system in a wide range of behavioural and neurobiological studies. The increasing number of genetic tools available for this species, together with the emerging interest in its use for neurobiological studies, increased the need for an accurate hodological mapping of the tilapia brain to supplement the available histological data. The goal of our study was to elaborate a three-dimensional, high-resolution digital atlas using magnetic resonance imaging, supported by Nissl staining. Resulting images were viewed and analysed in all orientations (transverse, sagittal, and horizontal) and manually labelled to reveal structures in the olfactory bulb, telencephalon, diencephalon, optic tectum, and cerebellum. This high resolution tilapia brain atlas is expected to become a very useful tool for neuroscientists using this fish model and will certainly expand their use in future studies regarding the central nervous system.

Femtosecond laser micro-inscription of optical coherence tomography resolution test artifacts.

PubMed

Tomlins, Peter H; Smith, Graham N; Woolliams, Peter D; Rasakanthan, Janarthanan; Sugden, Kate

2011-04-25

Optical coherence tomography (OCT) systems are becoming more commonly used in biomedical imaging and, to enable continued uptake, a reliable method of characterizing their performance and validating their operation is required. This paper outlines the use of femtosecond laser subsurface micro-inscription techniques to fabricate an OCT test artifact for validating the resolution performance of a commercial OCT system. The key advantage of this approach is that by utilizing the nonlinear absorption a three dimensional grid of highly localized point and line defects can be written in clear fused silica substrates.

Resolution extension by image summing in serial femtosecond crystallography of two-dimensional membrane-protein crystals

DOE PAGES

Casadei, Cecilia M.; Tsai, Ching-Ju; Barty, Anton; ...

2018-01-01

Previous proof-of-concept measurements on single-layer two-dimensional membrane-protein crystals performed at X-ray free-electron lasers (FELs) have demonstrated that the collection of meaningful diffraction patterns, which is not possible at synchrotrons because of radiation-damage issues, is feasible. Here, the results obtained from the analysis of a thousand single-shot, room-temperature X-ray FEL diffraction images from two-dimensional crystals of a bacteriorhodopsin mutant are reported in detail. The high redundancy in the measurements boosts the intensity signal-to-noise ratio, so that the values of the diffracted intensities can be reliably determined down to the detector-edge resolution of 4 Å. The results show that two-dimensional serial crystallography atmore » X-ray FELs is a suitable method to study membrane proteins to near-atomic length scales at ambient temperature. The method presented here can be extended to pump–probe studies of optically triggered structural changes on submillisecond timescales in two-dimensional crystals, which allow functionally relevant large-scale motions that may be quenched in three-dimensional crystals.« less

Resolution extension by image summing in serial femtosecond crystallography of two-dimensional membrane-protein crystals

DOE Office of Scientific and Technical Information (OSTI.GOV)

Casadei, Cecilia M.; Tsai, Ching-Ju; Barty, Anton

Previous proof-of-concept measurements on single-layer two-dimensional membrane-protein crystals performed at X-ray free-electron lasers (FELs) have demonstrated that the collection of meaningful diffraction patterns, which is not possible at synchrotrons because of radiation-damage issues, is feasible. Here, the results obtained from the analysis of a thousand single-shot, room-temperature X-ray FEL diffraction images from two-dimensional crystals of a bacteriorhodopsin mutant are reported in detail. The high redundancy in the measurements boosts the intensity signal-to-noise ratio, so that the values of the diffracted intensities can be reliably determined down to the detector-edge resolution of 4 Å. The results show that two-dimensional serial crystallography atmore » X-ray FELs is a suitable method to study membrane proteins to near-atomic length scales at ambient temperature. The method presented here can be extended to pump–probe studies of optically triggered structural changes on submillisecond timescales in two-dimensional crystals, which allow functionally relevant large-scale motions that may be quenched in three-dimensional crystals.« less

Thin polymer etalon arrays for high-resolution photoacoustic imaging

PubMed Central

Hou, Yang; Huang, Sheng-Wen; Ashkenazi, Shai; Witte, Russell; O’Donnell, Matthew

2009-01-01

Thin polymer etalons are demonstrated as high-frequency ultrasound sensors for three-dimensional (3-D) high-resolution photoacoustic imaging. The etalon, a Fabry-Perot optical resonator, consists of a thin polymer slab sandwiched between two gold layers. It is probed with a scanning continuous-wave (CW) laser for ultrasound array detection. Detection bandwidth of a 20-μm-diam array element exceeds 50 MHz, and the ultrasound sensitivity is comparable to polyvinylidene fluoride (PVDF) equivalents of similar size. In a typical photoacoustic imaging setup, a pulsed laser beam illuminates the imaging target, where optical energy is absorbed and acoustic waves are generated through the thermoelastic effect. An ultrasound detection array is formed by scanning the probing laser beam on the etalon surface in either a 1-D or a 2-D configuration, which produces 2-D or 3-D images, respectively. Axial and lateral resolutions have been demonstrated to be better than 20 μm. Detailed characterizations of the optical and acoustical properties of the etalon, as well as photoacoustic imaging results, suggest that thin polymer etalon arrays can be used as ultrasound detectors for 3-D high-resolution photoacoustic imaging applications. PMID:19123679

Three-dimensional integral imaging displays using a quick-response encoded elemental image array: an overview

NASA Astrophysics Data System (ADS)

Markman, A.; Javidi, B.

2016-06-01

Quick-response (QR) codes are barcodes that can store information such as numeric data and hyperlinks. The QR code can be scanned using a QR code reader, such as those built into smartphone devices, revealing the information stored in the code. Moreover, the QR code is robust to noise, rotation, and illumination when scanning due to error correction built in the QR code design. Integral imaging is an imaging technique used to generate a three-dimensional (3D) scene by combining the information from two-dimensional (2D) elemental images (EIs) each with a different perspective of a scene. Transferring these 2D images in a secure manner can be difficult. In this work, we overview two methods to store and encrypt EIs in multiple QR codes. The first method uses run-length encoding with Huffman coding and the double-random-phase encryption (DRPE) to compress and encrypt an EI. This information is then stored in a QR code. An alternative compression scheme is to perform photon-counting on the EI prior to compression. Photon-counting is a non-linear transformation of data that creates redundant information thus improving image compression. The compressed data is encrypted using the DRPE. Once information is stored in the QR codes, it is scanned using a smartphone device. The information scanned is decompressed and decrypted and an EI is recovered. Once all EIs have been recovered, a 3D optical reconstruction is generated.

Common-path low-coherence interferometry fiber-optic sensor guided microincision

NASA Astrophysics Data System (ADS)

Zhang, Kang; Kang, Jin U.

2011-09-01

We propose and demonstrate a common-path low-coherence interferometry (CP-LCI) fiber-optic sensor guided precise microincision. The method tracks the target surface and compensates the tool-to-surface relative motion with better than +/-5 μm resolution using a precision micromotor connected to the tool tip. A single-fiber distance probe integrated microdissector was used to perform an accurate 100 μm incision into the surface of an Intralipid phantom. The CP-LCI guided incision quality in terms of depth was evaluated afterwards using three-dimensional Fourier-domain optical coherence tomography imaging, which showed significant improvement of incision accuracy compared to free-hand-only operations.

Broadband full-color multichannel hologram with geometric metasurface.

PubMed

Qin, F F; Liu, Z Z; Zhang, Z; Zhang, Q; Xiao, J J

2018-04-30

Due to the abilities of manipulating the wavefront of light with well-controlled amplitude, and phase and polarization, optical metasurfaces are very suitable for optical holography, enabling applications with multiple functionalities and high data capacity. Here, we demonstrate encoding two- and three-dimensional full-color holographic images by an ultrathin metasurface hologram whose unit cells are subwavelength nanoslits with spatially varying orientations. We further show that it is possible to achieve full-color holographic multiplexing with such kind of geometric metasurfaces, realized by a synthetic spectrum holographic algorithm. Our results provide an efficient way to design multi-color optical display elements that are ready for fabrication.

Design and validation of a tissue bath 3-D printed with PLA for optically mapping suspended whole heart preparations.

PubMed

Entz, Michael; King, D Ryan; Poelzing, Steven

2017-12-01

With the sudden increase in affordable manufacturing technologies, the relationship between experimentalists and the designing process for laboratory equipment is rapidly changing. While experimentalists are still dependent on engineers and manufacturers for precision electrical, mechanical, and optical equipment, it has become a realistic option for in house manufacturing of other laboratory equipment with less precise design requirements. This is possible due to decreasing costs and increasing functionality of desktop three-dimensional (3-D) printers and 3-D design software. With traditional manufacturing methods, iterative design processes are expensive and time consuming, and making more than one copy of a custom piece of equipment is prohibitive. Here, we provide an overview to design a tissue bath and stabilizer for a customizable, suspended, whole heart optical mapping apparatus that can be produced significantly faster and less expensive than conventional manufacturing techniques. This was accomplished through a series of design steps to prevent fluid leakage in the areas where the optical imaging glass was attached to the 3-D printed bath. A combination of an acetone dip along with adhesive was found to create a water tight bath. Optical mapping was used to quantify cardiac conduction velocity and action potential duration to compare 3-D printed baths to a bath that was designed and manufactured in a machine shop. Importantly, the manufacturing method did not significantly affect conduction, action potential duration, or contraction, suggesting that 3-D printed baths are equally effective for optical mapping experiments. NEW & NOTEWORTHY This article details three-dimensional printable equipment for use in suspended whole heart optical mapping experiments. This equipment is less expensive than conventional manufactured equipment as well as easily customizable to the experimentalist. The baths can be waterproofed using only a three-dimensional printer, acetone, a glass microscope slide, c-clamps, and adhesive. Copyright © 2017 the American Physiological Society.

Multimodal ophthalmic imaging using swept source spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography

NASA Astrophysics Data System (ADS)

Malone, Joseph D.; El-Haddad, Mohamed T.; Tye, Logan A.; Majeau, Lucas; Godbout, Nicolas; Rollins, Andrew M.; Boudoux, Caroline; Tao, Yuankai K.

2016-03-01

Scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT) benefit clinical diagnostic imaging in ophthalmology by enabling in vivo noninvasive en face and volumetric visualization of retinal structures, respectively. Spectrally encoding methods enable confocal imaging through fiber optics and reduces system complexity. Previous applications in ophthalmic imaging include spectrally encoded confocal scanning laser ophthalmoscopy (SECSLO) and a combined SECSLO-OCT system for image guidance, tracking, and registration. However, spectrally encoded imaging suffers from speckle noise because each spectrally encoded channel is effectively monochromatic. Here, we demonstrate in vivo human retinal imaging using a swept source spectrally encoded scanning laser ophthalmoscope and OCT (SSSESLO- OCT) at 1060 nm. SS-SESLO-OCT uses a shared 100 kHz Axsun swept source, shared scanner and imaging optics, and are detected simultaneously on a shared, dual channel high-speed digitizer. SESLO illumination and detection was performed using the single mode core and multimode inner cladding of a double clad fiber coupler, respectively, to preserve lateral resolution while improving collection efficiency and reducing speckle contrast at the expense of confocality. Concurrent en face SESLO and cross-sectional OCT images were acquired with 1376 x 500 pixels at 200 frames-per-second. Our system design is compact and uses a shared light source, imaging optics, and digitizer, which reduces overall system complexity and ensures inherent co-registration between SESLO and OCT FOVs. En face SESLO images acquired concurrent with OCT cross-sections enables lateral motion tracking and three-dimensional volume registration with broad applications in multivolume OCT averaging, image mosaicking, and intraoperative instrument tracking.

Volumetric three-dimensional display system with rasterization hardware

NASA Astrophysics Data System (ADS)

Favalora, Gregg E.; Dorval, Rick K.; Hall, Deirdre M.; Giovinco, Michael; Napoli, Joshua

2001-06-01

An 8-color multiplanar volumetric display is being developed by Actuality Systems, Inc. It will be capable of utilizing an image volume greater than 90 million voxels, which we believe is the greatest utilizable voxel set of any volumetric display constructed to date. The display is designed to be used for molecular visualization, mechanical CAD, e-commerce, entertainment, and medical imaging. As such, it contains a new graphics processing architecture, novel high-performance line- drawing algorithms, and an API similar to a current standard. Three-dimensional imagery is created by projecting a series of 2-D bitmaps ('image slices') onto a diffuse screen that rotates at 600 rpm. Persistence of vision fuses the slices into a volume-filling 3-D image. A modified three-panel Texas Instruments projector provides slices at approximately 4 kHz, resulting in 8-color 3-D imagery comprised of roughly 200 radially-disposed slices which are updated at 20 Hz. Each slice has a resolution of 768 by 768 pixels, subtending 10 inches. An unusual off-axis projection scheme incorporating tilted rotating optics is used to maintain good focus across the projection screen. The display electronics includes a custom rasterization architecture which converts the user's 3- D geometry data into image slices, as well as 6 Gbits of DDR SDRAM graphics memory.

Utility of three-dimensional and multiplanar reformatted computed tomography for evaluation of pediatric congenital spine abnormalities.

PubMed

Newton, Peter O; Hahn, Gregory W; Fricka, Kevin B; Wenger, Dennis R

2002-04-15

A retrospective radiographic review of 31 patients with congenital spine abnormalities who underwent conventional radiography and advanced imaging studies was conducted. To analyze the utility of three-dimensional computed tomography with multiplanar reformatted images for congenital spine anomalies, as compared with plain radiographs and axial two-dimensional computed tomography imaging. Conventional radiographic imaging for congenital spine disorders often are difficult to interpret because of the patient's small size, the complexity of the disorder, a deformity not in the plane of the radiographs, superimposed structures, and difficulty in forming a mental three-dimensional image. Multiplanar reformatted and three-dimensional computed tomographic imaging offers many potential advantages for defining congenital spine anomalies including visualization of the deformity in any plane, from any angle, with the overlying structures subtracted. The imaging studies of patients who had undergone a three-dimensional computed tomography for congenital deformities of the spine between 1992 and 1998 were reviewed (31 cases). All plain radiographs and axial two-dimensional computed tomography images performed before the three-dimensional computed tomography were reviewed and the findings documented. This was repeated for the three-dimensional reconstructions and, when available, the multiplanar reformatted images (15 cases). In each case, the utility of the advanced imaging was graded as one of the following: Grade A (substantial new information obtained), Grade B (confirmatory with improved visualization and understanding of the deformity), and Grade C (no added useful information obtained). In 17 of 31 cases, the multiplanar reformatted and three-dimensional images allowed identification of unrecognized malformations. In nine additional cases, the advanced imaging was helpful in better visualizing and understanding previously identified deformities. In five cases, no new information was gained. The standard and curved multiplanar reformatted images were best for defining the occiput-C1-C2 anatomy and the extent of segmentation defects. The curved multiplanar reformatted images were especially helpful in keeping the spine from "coming in" and "going out" of the plane of the image when there was significant spine deformity in the sagittal or coronal plane. The three-dimensional reconstructions proved valuable in defining failures of formation. Advanced computed tomography imaging (three-dimensional computed tomography and curved/standard multiplanar reformatted images) allows better definition of congenital spine anomalies. More than 50% of the cases showed additional abnormalities not appreciated on plain radiographs or axial two-dimensional computed tomography images. Curved multiplanar reformatted images allowed imaging in the coronal and sagittal planes of the entire deformity.

Phase in Optical Image Processing

NASA Astrophysics Data System (ADS)

Naughton, Thomas J.

2010-04-01

The use of phase has a long standing history in optical image processing, with early milestones being in the field of pattern recognition, such as VanderLugt's practical construction technique for matched filters, and (implicitly) Goodman's joint Fourier transform correlator. In recent years, the flexibility afforded by phase-only spatial light modulators and digital holography, for example, has enabled many processing techniques based on the explicit encoding and decoding of phase. One application area concerns efficient numerical computations. Pushing phase measurement to its physical limits, designs employing the physical properties of phase have ranged from the sensible to the wonderful, in some cases making computationally easy problems easier to solve and in other cases addressing mathematics' most challenging computationally hard problems. Another application area is optical image encryption, in which, typically, a phase mask modulates the fractional Fourier transformed coefficients of a perturbed input image, and the phase of the inverse transform is then sensed as the encrypted image. The inherent linearity that makes the system so elegant mitigates against its use as an effective encryption technique, but we show how a combination of optical and digital techniques can restore confidence in that security. We conclude with the concept of digital hologram image processing, and applications of same that are uniquely suited to optical implementation, where the processing, recognition, or encryption step operates on full field information, such as that emanating from a coherently illuminated real-world three-dimensional object.

Photothermal optical coherence tomography for depth-resolved imaging of mesenchymal stem cells via single wall carbon nanotubes

NASA Astrophysics Data System (ADS)

Subhash, Hrebesh M.; Connolly, Emma; Murphy, Mary; Barron, Valerie; Leahy, Martin

2014-03-01

The progress in stem cell research over the past decade holds promise and potential to address many unmet clinical therapeutic needs. Tracking stem cell with modern imaging modalities are critically needed for optimizing stem cell therapy, which offers insight into various underlying biological processes such as cell migration, engraftment, homing, differentiation, and functions etc. In this study we report the feasibility of photothermal optical coherence tomography (PT-OCT) to image human mesenchymal stem cells (hMSCs) labeled with single-walled carbon nanotubes (SWNTs) for in vitro cell tracking in three dimensional scaffolds. PT-OCT is a functional extension of conventional OCT with extended capability of localized detection of absorbing targets from scattering background to provide depth-resolved molecular contrast imaging. A 91 kHz line rate, spectral domain PT-OCT system at 1310nm was developed to detect the photothermal signal generated by 800nm excitation laser. In general, MSCs do not have obvious optical absorption properties and cannot be directly visualized using PT-OCT imaging. However, the optical absorption properties of hMSCs can me modified by labeling with SWNTs. Using this approach, MSC were labeled with SWNT and the cell distribution imaged in a 3D polymer scaffold using PT-OCT.

Three-dimensional laser microvision.

PubMed

Shimotahira, H; Iizuka, K; Chu, S C; Wah, C; Costen, F; Yoshikuni, Y

2001-04-10

A three-dimensional (3-D) optical imaging system offering high resolution in all three dimensions, requiring minimum manipulation and capable of real-time operation, is presented. The system derives its capabilities from use of the superstructure grating laser source in the implementation of a laser step frequency radar for depth information acquisition. A synthetic aperture radar technique was also used to further enhance its lateral resolution as well as extend the depth of focus. High-speed operation was made possible by a dual computer system consisting of a host and a remote microcomputer supported by a dual-channel Small Computer System Interface parallel data transfer system. The system is capable of operating near real time. The 3-D display of a tunneling diode, a microwave integrated circuit, and a see-through image taken by the system operating near real time are included. The depth resolution is 40 mum; lateral resolution with a synthetic aperture approach is a fraction of a micrometer and that without it is approximately 10 mum.

Development of Three-Dimensional Dental Scanning Apparatus Using Structured Illumination

PubMed Central

Park, Anjin; Lee, Byeong Ha; Eom, Joo Beom

2017-01-01

We demonstrated a three-dimensional (3D) dental scanning apparatus based on structured illumination. A liquid lens was used for tuning focus and a piezomotor stage was used for the shift of structured light. A simple algorithm, which detects intensity modulation, was used to perform optical sectioning with structured illumination. We reconstructed a 3D point cloud, which represents the 3D coordinates of the digitized surface of a dental gypsum cast by piling up sectioned images. We performed 3D registration of an individual 3D point cloud, which includes alignment and merging the 3D point clouds to exhibit a 3D model of the dental cast. PMID:28714897

Extracting and compensating dispersion mismatch in ultrahigh-resolution Fourier domain OCT imaging of the retina

PubMed Central

Choi, WooJhon; Baumann, Bernhard; Swanson, Eric A.; Fujimoto, James G.

2012-01-01

We present a numerical approach to extract the dispersion mismatch in ultrahigh-resolution Fourier domain optical coherence tomography (OCT) imaging of the retina. The method draws upon an analogy with a Shack-Hartmann wavefront sensor. By exploiting mathematical similarities between the expressions for aberration in optical imaging and dispersion mismatch in spectral / Fourier domain OCT, Shack-Hartmann principles can be extended from the two-dimensional paraxial wavevector space (or the x-y plane in the spatial domain) to the one-dimensional wavenumber space (or the z-axis in the spatial domain). For OCT imaging of the retina, different retinal layers, such as the retinal nerve fiber layer (RNFL), the photoreceptor inner and outer segment junction (IS/OS), or all the retinal layers near the retinal pigment epithelium (RPE) can be used as point source beacons in the axial direction, analogous to point source beacons used in conventional two-dimensional Shack-Hartman wavefront sensors for aberration characterization. Subtleties regarding speckle phenomena in optical imaging, which affect the Shack-Hartmann wavefront sensor used in adaptive optics, also occur analogously in this application. Using this approach and carefully suppressing speckle, the dispersion mismatch in spectral / Fourier domain OCT retinal imaging can be successfully extracted numerically and used for numerical dispersion compensation to generate sharper, ultrahigh-resolution OCT images. PMID:23187353

Modeling of the gain distribution for diode pumping of a solid-state laser rod with nonimaging optics.

PubMed

Koshel, R J; Walmsley, I A

1993-03-20

We investigate the absorption distribution in a cylindrical gain medium that is pumped by a source of distributed laser diodes by means of a pump cavity developed from the edge-ray principle of nonimaging optics. The performance of this pumping arrangement is studied by using a nonsequential, numerical, three-dimensional ray-tracing scheme. A figure of merit is defined for the pump cavities that takes into account the coupling efficiency and uniformity of the absorption distribution. It is found that the nonimaging pump cavity maintains a high coupling efficiency with extended two-dimensional diode arrays and obtains a fairly uniform absorption distribution. The nonimaging cavity is compared with two other designs: a close-coupled side-pumped cavity and an imaging design in the form of a elliptical cavity. The nonimaging cavity has a better figure of merit per diode than these two designs. It also permits the use of an extended, sparse, two-dimensional diode array, which reduces thermal loading of the source and eliminates all cavity optics other than the main reflector.

Imaging galectin-3 dependent endocytosis with lattice light-sheet microscopy

NASA Astrophysics Data System (ADS)

Baek, Jongho; Lou, Jieqiong; Coelho, Simao; Lim, Yean Jin; Seidlitz, Silvia; Nicovich, Philip R.; Wunder, Christian; Johannes, Ludger; Gaus, Katharina

2017-04-01

Lattice light-sheet (LLS) microscopy provides ultrathin light sheets of a two-dimensional optical lattice that allows us imaging three-dimensional (3D) objects for hundreds of time points at sub-second intervals and at or below the diffraction limit. Galectin-3 (Gal3), a carbohydrate-binding protein, triggers glycosphingolipid (GSL)-dependent biogenesis of morphologically distinct endocytic vesicles that are cargo specific and clathrin independent. In this study, we apply LLS microscopy to study the dynamics of Gal3 dependent endocytosis in live T cells. This will allow us to observe Gal3-mediated endocytosis at high temporal and excellent 3D spatial resolution, which may shed light on our understanding of the mechanism and physiological function of Gal3-induced endocytosis.

A web service system supporting three-dimensional post-processing of medical images based on WADO protocol.

PubMed

He, Longjun; Xu, Lang; Ming, Xing; Liu, Qian

2015-02-01

Three-dimensional post-processing operations on the volume data generated by a series of CT or MR images had important significance on image reading and diagnosis. As a part of the DIOCM standard, WADO service defined how to access DICOM objects on the Web, but it didn't involve three-dimensional post-processing operations on the series images. This paper analyzed the technical features of three-dimensional post-processing operations on the volume data, and then designed and implemented a web service system for three-dimensional post-processing operations of medical images based on the WADO protocol. In order to improve the scalability of the proposed system, the business tasks and calculation operations were separated into two modules. As results, it was proved that the proposed system could support three-dimensional post-processing service of medical images for multiple clients at the same moment, which met the demand of accessing three-dimensional post-processing operations on the volume data on the web.

Full color natural light holographic camera.

PubMed

Kim, Myung K

2013-04-22

Full-color, three-dimensional images of objects under incoherent illumination are obtained by a digital holography technique. Based on self-interference of two beam-split copies of the object's optical field with differential curvatures, the apparatus consists of a beam-splitter, a few mirrors and lenses, a piezo-actuator, and a color camera. No lasers or other special illuminations are used for recording or reconstruction. Color holographic images of daylight-illuminated outdoor scenes and a halogen lamp-illuminated toy figure are obtained. From a recorded hologram, images can be calculated, or numerically focused, at any distances for viewing.

Laser tweezer actuated microphotonic array devices for high resolution imaging and analysis in chip-based biosystems

NASA Astrophysics Data System (ADS)

Birkbeck, Aaron L.

A new technology is developed that functionally integrates arrays of lasers and micro-optics into microfluidic systems for the purpose of imaging, analyzing, and manipulating objects and biological cells. In general, the devices and technologies emerging from this area either lack functionality through the reliance on mechanical systems or provide a serial-based, time consuming approach. As compared to the current state of art, our all-optical design methodology has several distinguishing features, such as parallelism, high efficiency, low power, auto-alignment, and high yield fabrication methods, which all contribute to minimizing the cost of the integration process. The potential use of vertical cavity surface emitting lasers (VCSELs) for the creation of two-dimensional arrays of laser optical tweezers that perform independently controlled, parallel capture, and transport of large numbers of individual objects and biological cells is investigated. One of the primary biological applications for which VCSEL array sourced laser optical tweezers are considered is the formation of engineered tissues through the manipulation and spatial arrangement of different types of cells in a co-culture. Creating devices that combine laser optical tweezers with select micro-optical components permits optical imaging and analysis functions to take place inside the microfluidic channel. One such device is a micro-optical spatial filter whose motion and alignment is controlled using a laser optical tweezer. Unlike conventional spatial filter systems, our device utilizes a refractive optical element that is directly incorporated onto the lithographically patterned spatial filter. This allows the micro-optical spatial filter to automatically align itself in three-dimensions to the focal point of the microscope objective, where it then filters out the higher frequency additive noise components present in the laser beam. As a means of performing high resolution imaging in the microfluidic channel, we developed a novel technique that integrates the capacity of a laser tweezer to optically trap and manipulate objects in three-dimensions with the resolution-enhanced imaging capabilities of a solid immersion lens (SIL). In our design, the SIL is a free-floating device whose imaging beam, motion control and alignment is provided by a laser optical tweezer, which allows the microfluidic SIL to image in areas that are inaccessible to traditional solid immersion microscopes.

Three-body Annihilation at the Onset of Anomalous Photocurrent Suppression in Vertical Heterostrucutres of MoTe2

NASA Astrophysics Data System (ADS)

Arp, Trevor; Pleskot, Dennis; Gabor, Nathaniel

We have developed a new photoresponse imaging technique that utilizes extensive data acquisition over a large parameter space. By acquiring a multi-dimensional data set, we fully capture the intrinsic optoelectronic response of two-dimensional heterostructure devices. Using this technique we have investigated the behavior of heterostructures consisting of molybdenum ditelluride (MoTe2) sandwiched between graphene top and bottom contacts. Under near-infrared optical excitation, the ultra-thin heterostructure devices exhibit sub-linear photocurrent response that recovers within several dozen picoseconds. As the optical power increases, the dynamics of the photoresponse, consistent with 3-body annihilation, precede a sudden suppression of photocurrent. The observed dynamics near the threshold to photocurrent suppression may indicate the onset to a strongly interacting population of electrons and holes.

3D-Holoscopic Imaging: A New Dimension to Enhance Imaging in Minimally Invasive Therapy in Urologic Oncology

PubMed Central

Aggoun, Amar; Swash, Mohammad; Grange, Philippe C.R.; Challacombe, Benjamin; Dasgupta, Prokar

2013-01-01

Abstract Background and Purpose Existing imaging modalities of urologic pathology are limited by three-dimensional (3D) representation on a two-dimensional screen. We present 3D-holoscopic imaging as a novel method of representing Digital Imaging and Communications in Medicine data images taken from CT and MRI to produce 3D-holographic representations of anatomy without special eyewear in natural light. 3D-holoscopic technology produces images that are true optical models. This technology is based on physical principles with duplication of light fields. The 3D content is captured in real time with the content viewed by multiple viewers independently of their position, without 3D eyewear. Methods We display 3D-holoscopic anatomy relevant to minimally invasive urologic surgery without the need for 3D eyewear. Results The results have demonstrated that medical 3D-holoscopic content can be displayed on commercially available multiview auto-stereoscopic display. Conclusion The next step is validation studies comparing 3D-Holoscopic imaging with conventional imaging. PMID:23216303

Adaptive optics and the eye (super resolution OCT).

PubMed

Miller, D T; Kocaoglu, O P; Wang, Q; Lee, S

2011-03-01

The combination of adaptive optics (AO) and optical coherence tomography (OCT) was first reported 8 years ago and has undergone tremendous technological advances since then. The technical benefits of adding AO to OCT (increased lateral resolution, smaller speckle, and enhanced sensitivity) increase the imaging capability of OCT in ways that make it well suited for three-dimensional (3D) cellular imaging in the retina. Today, AO-OCT systems provide ultrahigh 3D resolution (3 × 3 × 3 μm³) and ultrahigh speed (up to an order of magnitude faster than commercial OCT). AO-OCT systems have been used to capture volume images of retinal structures, previously only visible with histology, and are being used for studying clinical conditions. Here, we present representative examples of cellular structures that can be visualized with AO-OCT. We overview three studies from our laboratory that used ultrahigh-resolution AO-OCT to measure the cross-sectional profiles of individual bundles in the retinal nerve fiber layer; the diameters of foveal capillaries that define the terminal rim of the foveal avascular zone; and the spacing and length of individual cone photoreceptor outer segments as close as 0.5° from the fovea center.

Quantitative assessment of biophotonic imaging system performance with phantoms fabricated by rapid prototyping

NASA Astrophysics Data System (ADS)

Wang, Jianting; Coburn, James; Woolsey, Nicholas; Liang, Chia-Pin; Ramella-Roman, Jessica; Chen, Yu; Pfefer, Joshua

2014-03-01

In biophotonic imaging, turbid phantoms that are low-cost, biologically-relevant, and durable are desired for standardized performance assessment. Such phantoms often contain inclusions of varying depths and sizes in order to quantify key image quality characteristics such as penetration depth, sensitivity and contrast detectability. The emerging technique of rapid prototyping with three-dimensional (3D) printers provides a potentially revolutionary way to fabricate these structures. Towards this goal, we have characterized the optical properties and morphology of phantoms fabricated by two 3D printing approaches: thermosoftening and photopolymerization. Material optical properties were measured by spectrophotometry while the morphology of phantoms incorporating 0.2-1.0 mm diameter channels was studied by μCT, optical coherence tomography (OCT) and optical microscopy. A near-infrared absorbing dye and nanorods at several concentrations were injected into channels to evaluate detectability with a near-infrared hyperspectral reflectance imaging (HRI) system (650-1100 nm). Phantoms exhibited biologically-relevant scattering and low absorption across visible and near-infrared wavelengths. Although limitations in resolution were noted, channels with diameters of 0.4 mm or more could be reliably fabricated. The most significant problem noted was the porosity of phantoms generated with the thermosoftening-based printer. The aforementioned three imaging methods provided a valuable mix of insights into phantom morphology and may also be useful for detailed structural inspection of medical devices fabricated by rapid prototyping, such as customized implants. Overall, our findings indicate that 3D printing has significant potential as a method for fabricating well-characterized, standard phantoms for medical imaging modalities such as HRI.

A novel 1050nm handheld OCT imaging system for pediatric retinoblastoma patients: technology development and clinical study (Conference Presentation)

NASA Astrophysics Data System (ADS)

Nadiarnykh, Oleg; Moll, Annette C.; de Boer, Johannes F.

2016-03-01

We demonstrate a novel optical coherence tomography system specifically developed and validated for clinical imaging of retinoblastoma tumors in pediatric patients. The existing treatment options for this malignant tumor of the retina aim at reduction of tumor (re)growth risks, and vision preservation. The choice of optimal treatment strongly depends on skilled and detailed clinical assessment. Due to the limitations of the existing real-time diagnostic tools the patients at risk are periodically monitored with retinal imaging to confirm the absence of new tumor seedings. Three-dimensional visualization of tissue layer and microvasculature at improved axial and lateral resolution of interference-based OCT imaging provides sensitivity for detection of vital tumor tissue concurrent with local treatment. Our METC-approved system accommodates for the range of optical parameters of infants' eyes, and uses the 1050nm wavelength to access the deeper choroid layers of retina. The prototype is designed for patients in supine position under general anesthesia, where ergonomic handheld module is connected to fiber-based optical setup via umbilical cord. The system conforms to clinical safety requirements, including fully isolated low-voltage electric circuit. Focusing is performed with a mechanically tunable lens, where resolution is 6 µm axially, and varies with focusing at 10-18µm laterally. We will present optical design, performance limitations, and results of the ongoing clinical study, including the increased OCT diagnostic sensitivity in three dimensions in comparison with the established clinical imaging modalities. We will discuss images of early, active, and treated tumors, as well as follow-up on patients after local and systemic treatments.

Optical recognition of statistical patterns

NASA Astrophysics Data System (ADS)

Lee, S. H.

1981-12-01

Optical implementation of the Fukunaga-Koontz transform (FKT) and the Least-Squares Linear Mapping Technique (LSLMT) is described. The FKT is a linear transformation which performs image feature extraction for a two-class image classification problem. The LSLMT performs a transform from large dimensional feature space to small dimensional decision space for separating multiple image classes by maximizing the interclass differences while minimizing the intraclass variations. The FKT and the LSLMT were optically implemented by utilizing a coded phase optical processor. The transform was used for classifying birds and fish. After the F-K basis functions were calculated, those most useful for classification were incorporated into a computer generated hologram. The output of the optical processor, consisting of the squared magnitude of the F-K coefficients, was detected by a T.V. camera, digitized, and fed into a micro-computer for classification. A simple linear classifier based on only two F-K coefficients was able to separate the images into two classes, indicating that the F-K transform had chosen good features. Two advantages of optically implementing the FKT and LSLMT are parallel and real time processing.

Optical recognition of statistical patterns

NASA Technical Reports Server (NTRS)

Lee, S. H.

1981-01-01

Optical implementation of the Fukunaga-Koontz transform (FKT) and the Least-Squares Linear Mapping Technique (LSLMT) is described. The FKT is a linear transformation which performs image feature extraction for a two-class image classification problem. The LSLMT performs a transform from large dimensional feature space to small dimensional decision space for separating multiple image classes by maximizing the interclass differences while minimizing the intraclass variations. The FKT and the LSLMT were optically implemented by utilizing a coded phase optical processor. The transform was used for classifying birds and fish. After the F-K basis functions were calculated, those most useful for classification were incorporated into a computer generated hologram. The output of the optical processor, consisting of the squared magnitude of the F-K coefficients, was detected by a T.V. camera, digitized, and fed into a micro-computer for classification. A simple linear classifier based on only two F-K coefficients was able to separate the images into two classes, indicating that the F-K transform had chosen good features. Two advantages of optically implementing the FKT and LSLMT are parallel and real time processing.

Stochastic optical reconstruction microscopy-based relative localization analysis (STORM-RLA) for quantitative nanoscale assessment of spatial protein organization.

PubMed

Veeraraghavan, Rengasayee; Gourdie, Robert G

2016-11-07

The spatial association between proteins is crucial to understanding how they function in biological systems. Colocalization analysis of fluorescence microscopy images is widely used to assess this. However, colocalization analysis performed on two-dimensional images with diffraction-limited resolution merely indicates that the proteins are within 200-300 nm of each other in the xy-plane and within 500-700 nm of each other along the z-axis. Here we demonstrate a novel three-dimensional quantitative analysis applicable to single-molecule positional data: stochastic optical reconstruction microscopy-based relative localization analysis (STORM-RLA). This method offers significant advantages: 1) STORM imaging affords 20-nm resolution in the xy-plane and <50 nm along the z-axis; 2) STORM-RLA provides a quantitative assessment of the frequency and degree of overlap between clusters of colabeled proteins; and 3) STORM-RLA also calculates the precise distances between both overlapping and nonoverlapping clusters in three dimensions. Thus STORM-RLA represents a significant advance in the high-throughput quantitative assessment of the spatial organization of proteins. © 2016 Veeraraghavan and Gourdie. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

Three-dimensional scanning near field optical microscopy (3D-SNOM) imaging of random arrays of copper nanoparticles: implications for plasmonic solar cell enhancement.

PubMed

Ezugwu, Sabastine; Ye, Hanyang; Fanchini, Giovanni

2015-01-07

In order to investigate the suitability of random arrays of nanoparticles for plasmonic enhancement in the visible-near infrared range, we introduced three-dimensional scanning near-field optical microscopy (3D-SNOM) imaging as a useful technique to probe the intensity of near-field radiation scattered by random systems of nanoparticles at heights up to several hundred nm from their surface. We demonstrated our technique using random arrays of copper nanoparticles (Cu-NPs) at different particle diameter and concentration. Bright regions in the 3D-SNOM images, corresponding to constructive interference of forward-scattered plasmonic waves, were obtained at heights Δz ≥ 220 nm from the surface for random arrays of Cu-NPs of ∼ 60-100 nm in diameter. These heights are too large to use Cu-NPs in contact of the active layer for light harvesting in thin organic solar cells, which are typically no thicker than 200 nm. Using a 200 nm transparent spacer between the system of Cu-NPs and the solar cell active layer, we demonstrate that forward-scattered light can be conveyed in 200 nm thin film solar cells. This architecture increases the solar cell photoconversion efficiency by a factor of 3. Our 3D-SNOM technique is general enough to be suitable for a large number of other applications in nanoplasmonics.

Full-color digitized holography for large-scale holographic 3D imaging of physical and nonphysical objects.

PubMed

Matsushima, Kyoji; Sonobe, Noriaki

2018-01-01

Digitized holography techniques are used to reconstruct three-dimensional (3D) images of physical objects using large-scale computer-generated holograms (CGHs). The object field is captured at three wavelengths over a wide area at high densities. Synthetic aperture techniques using single sensors are used for image capture in phase-shifting digital holography. The captured object field is incorporated into a virtual 3D scene that includes nonphysical objects, e.g., polygon-meshed CG models. The synthetic object field is optically reconstructed as a large-scale full-color CGH using red-green-blue color filters. The CGH has a wide full-parallax viewing zone and reconstructs a deep 3D scene with natural motion parallax.

Visual stimulus presentation using fiber optics in the MRI scanner.

PubMed

Huang, Ruey-Song; Sereno, Martin I

2008-03-30

Imaging the neural basis of visuomotor actions using fMRI is a topic of increasing interest in the field of cognitive neuroscience. One challenge is to present realistic three-dimensional (3-D) stimuli in the subject's peripersonal space inside the MRI scanner. The stimulus generating apparatus must be compatible with strong magnetic fields and must not interfere with image acquisition. Virtual 3-D stimuli can be generated with a stereo image pair projected onto screens or via binocular goggles. Here, we describe designs and implementations for automatically presenting physical 3-D stimuli (point-light targets) in peripersonal and near-face space using fiber optics in the MRI scanner. The feasibility of fiber-optic based displays was demonstrated in two experiments. The first presented a point-light array along a slanted surface near the body, and the second presented multiple point-light targets around the face. Stimuli were presented using phase-encoded paradigms in both experiments. The results suggest that fiber-optic based displays can be a complementary approach for visual stimulus presentation in the MRI scanner.