Copper as a target for prostate cancer therapeutics: copper-ionophore pharmacology and altering systemic copper distribution

PubMed Central

Denoyer, Delphine; Pearson, Helen B.; Clatworthy, Sharnel A.S.; Smith, Zoe M.; Francis, Paul S.; Llanos, Roxana M.; Volitakis, Irene; Phillips, Wayne A.; Meggyesy, Peter M.; Masaldan, Shashank; Cater, Michael A.

2016-01-01

Copper-ionophores that elevate intracellular bioavailable copper display significant therapeutic utility against prostate cancer cells in vitro and in TRAMP (Transgenic Adenocarcinoma of Mouse Prostate) mice. However, the pharmacological basis for their anticancer activity remains unclear, despite impending clinical trails. Herein we show that intracellular copper levels in prostate cancer, evaluated in vitro and across disease progression in TRAMP mice, were not correlative with copper-ionophore activity and mirrored the normal levels observed in patient prostatectomy tissues (Gleason Score 7 & 9). TRAMP adenocarcinoma cells harbored markedly elevated oxidative stress and diminished glutathione (GSH)-mediated antioxidant capacity, which together conferred selective sensitivity to prooxidant ionophoric copper. Copper-ionophore treatments [CuII(gtsm), disulfiram & clioquinol] generated toxic levels of reactive oxygen species (ROS) in TRAMP adenocarcinoma cells, but not in normal mouse prostate epithelial cells (PrECs). Our results provide a basis for the pharmacological activity of copper-ionophores and suggest they are amendable for treatment of patients with prostate cancer. Additionally, recent in vitro and mouse xenograft studies have suggested an increased copper requirement by prostate cancer cells. We demonstrated that prostate adenocarcinoma development in TRAMP mice requires a functional supply of copper and is significantly impeded by altered systemic copper distribution. The presence of a mutant copper-transporting Atp7b protein (tx mutation: A4066G/Met1356Val) in TRAMP mice changed copper-integration into serum and caused a remarkable reduction in prostate cancer burden (64% reduction) and disease severity (grade), abrogating adenocarcinoma development. Implications for current clinical trials are discussed. PMID:27175597

Growth and Progression of TRAMP Prostate Tumors in Relationship to Diet and Obesity

PubMed Central

Bonorden, Melissa J. L.; Grossmann, Michael E.; Ewing, Sarah A.; Rogozina, Olga P.; Ray, Amitabha; Nkhata, Katai J.; Liao, D. Joshua; Grande, Joseph P.; Cleary, Margot P.

2012-01-01

To clarify effects of diet and body weight on prostate cancer development, three studies were undertaken using the TRAMP mouse model of this disease. In the first experiment, obesity was induced by injection of gold thioglucose (GTG). Age of prostate tumor detection (~33 wk) and death (~43 wk) was not significantly different among the groups. In the second study, TRAMP-C2 cells were injected into syngeneic C57BL6 mice and tumor progression was evaluated in mice fed either high-fat or low-fat diets. The high fat fed mice had larger tumors than did the low-fat fed mice. In the third study, tumor development was followed in TRAMP mice fed a high fat diet from 6 weeks of age. There were no significant effects of body weight status or diet on tumor development among the groups. When the tumors were examined for the neuroendocrine marker synaptophysin, there was no correlation with either body weight or diet. However, there was a significant correlation of the expression of synaptophysin with earlier age to tumor detection and death. In summary, TRAMP-C2 cells grew faster when the mice were fed a high-fat diet. Further synaptophysin may be a marker of poor prognosis independent of weight and diet. PMID:23304522

Long pentraxin-3 as an epithelial-stromal fibroblast growth factor-targeting inhibitor in prostate cancer.

PubMed

Ronca, Roberto; Alessi, Patrizia; Coltrini, Daniela; Di Salle, Emanuela; Giacomini, Arianna; Leali, Daria; Corsini, Michela; Belleri, Mirella; Tobia, Chiara; Garlanda, Cecilia; Bonomi, Elisa; Tardanico, Regina; Vermi, William; Presta, Marco

2013-06-01

Fibroblast growth factors (FGFs) exert autocrine/paracrine functions in prostate cancer by stimulating angiogenesis and tumour growth. Here dihydrotestosterone (DHT) up-regulates FGF2 and FGF8b production in murine TRAMP-C2 prostate cancer cells, activating a FGF-dependent autocrine loop of stimulation. The soluble pattern recognition receptor long pentraxin-3 (PTX3) acts as a natural FGF antagonist that binds FGF2 and FGF8b via its N-terminal domain. We demonstrate that recombinant PTX3 protein and the PTX3-derived pentapeptide Ac-ARPCA-NH2 abolish the mitogenic response of murine TRAMP-C2 cells and human LNCaP prostate cancer cells to DHT and FGFs. Also, PTX3 hampers the angiogenic activity of DHT-activated TRAMP-C2 cells on the chick embryo chorioallantoic membrane (CAM). Accordingly, human PTX3 overexpression inhibits the mitogenic activity exerted by DHT or FGFs on hPTX3_TRAMP-C2 cell transfectants and their angiogenic activity. Also, hPTX3_TRAMP-C2 cells show a dramatic decrease of their angiogenic and tumourigenic potential when grafted in syngeneic or immunodeficient athymic male mice. A similar inhibitory effect is observed when TRAMP-C2 cells overexpress only the FGF-binding N-terminal PTX3 domain. In keeping with the anti-tumour activity of PTX3 in experimental prostate cancer, immunohistochemical analysis of prostate needle biopsies from primary prostate adenocarcinoma patients shows that parenchymal PTX3 expression, abundant in basal cells of normal glands, is lost in high-grade prostatic intraepithelial neoplasia and in invasive tumour areas. These results identify PTX3 as a potent FGF antagonist endowed with anti-angiogenic and anti-neoplastic activity in prostate cancer. Copyright © 2013 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

Chemopreventive effects of Korean Angelica vs. its major pyranocoumarins on two lineages of transgenic adenocarcinoma of mouse prostate carcinogenesis

PubMed Central

Tang, Su-Ni; Zhang, Jinhui; Wu, Wei; Jiang, Peixin; Puppala, Manohar; Zhang, Yong; Xing, Chengguo; Kim, Sung-Hoon; Jiang, Cheng; Lü, Junxuan

2015-01-01

We showed previously that daily gavage of Angelica gigas Nakai (AGN) root ethanolic extract starting 8 weeks of age inhibited growth of prostate epithelium and neuroendocrine carcinomas (NE-Ca) in the transgenic adenocarcinoma of mouse prostate (TRAMP) model. Since decursin (D) and its isomer decursinol angelate (DA) are major pyranocoumarins in AGN extract, we tested the hypothesis that D/DA represented active/prodrug compounds against TRAMP carcinogenesis. Three groups of male C57BL/6 TRAMP mice were gavage-treated daily with excipient vehicle, AGN (5 mg per mouse) or equimolar D/DA (3 mg per mouse) from 8 weeks to 16 or 28 weeks of age. Measurement of plasma and NE-Ca D, DA and their common metabolite decursinol indicated similar retention from AGN vs. D/DA dosing. The growth of TRAMP dorsolateral prostate (DLP) in AGN-and D/DA-treated mice was inhibited by 66% and 61% at 16 weeks and by 67% and 72% at 28 weeks, respectively. Survival of mice bearing NE-Ca to 28 weeks was improved by AGN, but not by D/DA. Nevertheless, AGN-and D/DA-treated mice had lower NE-Ca burden. Immunohistochemical and mRNA analyses of DLP showed AGN and D/DA exerted similar inhibition of TRAMP epithelial lesion progression and key cell cycle genes. Profiling of NE-Ca mRNA showed a greater scope of modulating angiogenesis, epithelial-mesenchymal-transition, invasion-metastasis and inflammation genes by AGN than D/DA. The data therefore support D/DA as probable active/prodrug compounds against TRAMP epithelial lesions, and they cooperate with non-pyranocoumarin compounds to fully express AGN efficacy against NE-Ca. PMID:26116406

Chemopreventive Effects of Korean Angelica versus Its Major Pyranocoumarins on Two Lineages of Transgenic Adenocarcinoma of Mouse Prostate Carcinogenesis.

PubMed

Tang, Su-Ni; Zhang, Jinhui; Wu, Wei; Jiang, Peixin; Puppala, Manohar; Zhang, Yong; Xing, Chengguo; Kim, Sung-Hoon; Jiang, Cheng; Lü, Junxuan

2015-09-01

We showed previously that daily gavage of Angelica gigas Nakai (AGN) root ethanolic extract starting 8 weeks of age inhibited growth of prostate epithelium and neuroendocrine carcinomas (NE-Ca) in the transgenic adenocarcinoma of mouse prostate (TRAMP) model. Because decursin (D) and its isomer decursinol angelate (DA) are major pyranocoumarins in AGN extract, we tested the hypothesis that D/DA represented active/prodrug compounds against TRAMP carcinogenesis. Three groups of male C57BL/6 TRAMP mice were gavage treated daily with excipient vehicle, AGN (5 mg per mouse), or equimolar D/DA (3 mg per mouse) from 8 weeks to 16 or 28 weeks of age. Measurement of plasma and NE-Ca D, DA, and their common metabolite decursinol indicated similar retention from AGN versus D/DA dosing. The growth of TRAMP dorsolateral prostate (DLP) in AGN- and D/DA-treated mice was inhibited by 66% and 61% at 16 weeks and by 67% and 72% at 28 weeks, respectively. Survival of mice bearing NE-Ca to 28 weeks was improved by AGN, but not by D/DA. Nevertheless, AGN- and D/DA-treated mice had lower NE-Ca burden. Immunohistochemical and mRNA analyses of DLP showed that AGN and D/DA exerted similar inhibition of TRAMP epithelial lesion progression and key cell-cycle genes. Profiling of NE-Ca mRNA showed a greater scope of modulating angiogenesis, epithelial-mesenchymal transition, invasion-metastasis, and inflammation genes by AGN than D/DA. The data therefore support D/DA as probable active/prodrug compounds against TRAMP epithelial lesions, and they cooperate with non-pyranocoumarin compounds to fully express AGN efficacy against NE-Ca. ©2015 American Association for Cancer Research.

Leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4) is necessary for prostate cancer metastasis via epithelial-mesenchymal transition.

PubMed

Luo, Weijia; Tan, Peng; Rodriguez, Melissa; He, Lian; Tan, Kunrong; Zeng, Li; Siwko, Stefan; Liu, Mingyao

2017-09-15

Prostate cancer is a highly penetrant disease among men in industrialized societies, but the factors regulating the transition from indolent to aggressive and metastatic cancer remain poorly understood. We found that men with prostate cancers expressing high levels of the G protein-coupled receptor LGR4 had a significantly shorter recurrence-free survival compared with patients with cancers having low LGR4 expression. LGR4 expression was elevated in human prostate cancer cell lines with metastatic potential. We therefore generated a novel transgenic adenocarcinoma of the mouse prostate (TRAMP) mouse model to investigate the role of Lgr4 in prostate cancer development and metastasis in vivo TRAMP Lgr4 -/- mice exhibited an initial delay in prostate intraepithelial neoplasia formation, but the frequency of tumor formation was equivalent between TRAMP and TRAMP Lgr4 -/- mice by 12 weeks. The loss of Lgr4 significantly improved TRAMP mouse survival and dramatically reduced the occurrence of lung metastases. LGR4 knockdown impaired the migration, invasion, and colony formation of DU145 cells and reversed epithelial-mesenchymal transition (EMT), as demonstrated by up-regulation of E-cadherin and decreased expression of the EMT transcription factors ZEB, Twist, and Snail. Overexpression of LGR4 in LNCaP cells had the opposite effects. Orthotopic injection of DU145 cells stably expressing shRNA targeting LGR4 resulted in decreased xenograft tumor size, reduced tumor EMT marker expression, and impaired metastasis, in accord with our findings in TRAMP Lgr4 -/- mice. In conclusion, we propose that Lgr4 is a key protein necessary for prostate cancer EMT and metastasis. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Lack of Evidence for Green Tea Polyphenols as DNA Methylation Inhibitors in Murine Prostate

PubMed Central

Morey Kinney, Shannon R.; Zhang, Wa; Pascual, Marien; Greally, John M.; Gillard, Bryan M.; Karasik, Ellen; Foster, Barbara A.; Karpf, Adam R.

2009-01-01

Green tea polyphenols (GTPs) have been reported to inhibit DNA methylation in cultured cells. Here we tested whether oral consumption of GTPs affects normal or cancer specific DNA methylation in vivo, using mice. Wildtype (WT) and Transgenic Adenocarcinoma of Mouse Prostate (TRAMP) mice were administered 0.3% GTPs in drinking water beginning at 4 weeks of age. To monitor DNA methylation, we measured 5-methyl-deoxycytidine (5mdC) levels, methylation of the B1 repetitive element, and methylation of the Mage-a8 gene. Each of these parameters were unchanged in prostate, gut, and liver from WT mice at both 12 and 24 weeks of age, with the single exception of a decrease of 5mdC in the liver at 12 weeks. In GTP-treated TRAMP mice, 5mdC levels and the methylation status of four loci hypermethylated during tumor progression were unaltered in TRAMP prostates at 12 or 24 weeks. Quite surprisingly, GTP treatment did not inhibit tumor progression in TRAMP mice, although known pharmacodynamic markers of GTPs were altered in both WT and TRAMP prostates. We also administered 0.1%, 0.3%, or 0.6% GTPs to TRAMP mice for 12 weeks and measured 5mdC levels and methylation of B1 and Mage-a8 in prostate, gut, and liver tissues. No dose-dependent alterations in DNA methylation status were observed. Genome-wide DNA methylation profiling using the HELP assay also revealed no significant hypomethylating effect of GTP. These data indicate that oral administration of GTPs does not affect normal or cancer-specific DNA methylation in the murine prostate. PMID:19934341

Mixed Tocotrienols Inhibit Prostate Carcinogenesis in TRAMP Mice

PubMed Central

Barve, Avantika; Khor, Tin Oo; Reuhl, Kenneth; Reddy, Bandaru; Newmark, Harold; Kong, Ah-Ng

2015-01-01

The biological activities of tocotrienols are receiving increasing attention. Herein, we report the efficacy of a mixed-tocotrienol diet against prostate tumorigenesis in the transgenic adenocarcinoma mouse prostate (TRAMP) mouse model. Male TRAMP mice, 8 wk old, were fed 0.1%, 0.3%, or 1% mixed tocotrienols in AIN-76A diet up to 24 wk old. Likewise, a positive control group consisting of male TRAMP mice and a negative control group consisting of wild-type nontransgenic mice were fed regular AIN-76A diet up to 24 wk old. Our results show that mixed-tocotrienol-fed groups had a lower incidence of tumor formation along with a significant reduction in the average wet weight of genitourinary apparatus. Furthermore, mixed tocotrienols significantly reduced the levels of high-grade neoplastic lesions as compared to the positive controls. This decrease in levels of high-grade neoplastic lesions was found to be associated with increased expression of proapoptotic proteins BAD (Bcl2 antagonist of cell death) and cleaved caspase-3 and cell cycle regulatory proteins cyclin dependent kinase inhibitors p21 and p27. In contrast, the expression of cyclins A and E were found to be decreased in mixed-tocotrienol groups. Taken together, our results show that by modulating cell cycle regulatory proteins and increasing expression of proapoptotic proteins, mixed tocotrienols suppress prostate tumorigenesis in the TRAMP mice. PMID:20661828

First record of the tramp ant Cardiocondyla obscurior (Hymenoptera: Formicidae) for Mississippi

USDA-ARS?s Scientific Manuscript database

Cardiocondyla (Hymenoptera: Formicidae: Myrmicinae) is an old world genus of omnivorous ants native to Africa and Asia. The genus Cardiocondyla includes several common tramp species that have spread globally with human commerce. A single alate female C. obscurior Wheeler was collected by J. M. Stro...

TRAMP; The next generation data acquisition for RTP

DOE Office of Scientific and Technical Information (OSTI.GOV)

van Haren, P.C.; Wijnoltz, F.

1992-04-01

The Rijnhuizen Tokamak Project RTP is a medium-sized tokamak experiment, which requires a very reliable data-acquisition system, due to its pulsed nature. Analyzing the limitations of an existing CAMAC-based data-acquisition system showed, that substantial increase of performance and flexibility could best be obtained by the construction of an entirely new system. This paper discusses this system, CALLED TRAMP (Transient Recorder and Amoeba Multi Processor), based on tailor-made transient recorders with a multiprocessor computer system in VME running Amoeba. The performance of TRAMP exceeds the performance of the CAMAC system by a factor of four. The plans to increase the flexibilitymore » and for a further increase of performance are presented.« less

Decreased expression of Toll-like receptor 4 and 5 during progression of prostate transformation in transgenic adenocarcinoma of mouse prostate mice.

PubMed

Han, Ju-Hee; Park, Jong-Hwan; Kim, Bo-Yeon; Chang, Seo-Na; Kim, Tae-Hyoun; Park, Jae-Hak; Kim, Dong-Jae

2015-01-01

Chronic inflammation has been considered an important risk factor for development of prostate cancer. Toll-like receptors (TLRs) recognize microbial moieties or endogenous molecules and play an important role in the triggering and promotion of inflammation. In this study, we examined whether expression of TLR4 and TLR5 was associated with progression of prostate transformation in the transgenic adenocarcinoma of mouse prostate (TRAMP) model. The expression of TLR4 and TLR5 was evaluated by immunohistochemisty in formalin-fixed paraffin-embedded prostate tissue from wild-type (WT) and TRAMP mice. Normal prostate tissue from WT mice showed strong expression of TLR4 and TLR5. However, TLR4 expression in the prostate tissue from TRAMP mice gradually decreased as pathologic grade became more aggressive. TLR5 expression in the prostate tissue from TRAMP mice also decreased in low-grade prostate intraepithelial neoplasia (PIN), high-grade PIN and poorly differentiated adenocarcinoma. Overall, our results suggest that decreased expression of TLR4 and TLR5 may contribute to prostate tumorigenesis.

Tramping Trail with Elroy in the Early Years of CELP

ERIC Educational Resources Information Center

O'Krafka, Karen

2010-01-01

The author is sipping tea on the eve of a week-long winter trip--over a decade since she first tramped trail with Mike Elrick into the winter wilderness. This evening holds for her the same electricity that it did in 1997--anxiety and excitement--when Elroy guided a motley crew of teens (his gang) into the woods and frozen waters of Algonquin…

Polyphenois and Prostate Cancer Chemoprevention

DTIC Science & Technology

2006-03-01

The goal of this research is to investigate the potential of resveratrol genistein and (-) epigallocatechin -3- gallate ( EGCG ), alone in combination...to protect against prostate cancer in a transgenic rat model (TRAMP). The specific aims are 1) to investigate the potential of genistein, EGCG and...we have demonstrated that pure resveratrol in the diet, but not EGCG in the water, suppressed spontaneously developing prostate tumors in TRAMPs

Combination Therapy with a Second-Generation Androgen Receptor Antagonist and a Metastasis Vaccine Improves Survival in a Spontaneous Prostate Cancer Model

PubMed Central

Ardiani, Andressa; Farsaci, Benedetto; Rogers, Connie J.; Protter, Andy; Guo, Zhimin; King, Thomas H.; Apelian, David; Hodge, James W.

2013-01-01

Purpose Enzalutamide, a second-generation androgen antagonist, was approved by the FDA for castration-resistant prostate cancer (CRPC) treatment. Immunotherapy has been shown to be a promising strategy for prostate cancer. This study is performed to provide data to support the combination of enzalutamide and immunotherapy for CRPC treatment. Experimental Design Male C57BL/6 or TRAMP prostate cancer model mice were exposed to enzalutamide and/or a therapeutic vaccine targeting Twist, an antigen involved in epithelial-to-mesenchymal transition and metastasis. The physiological and immunological effects of enzalutamide were characterized. The generation of Twist-specific immunity by Twist-vaccine was evaluated. Finally, the combination of enzalutamide and Twist-vaccine to improve TRAMP mice overall survival was evaluated. Results Enzalutamide mediated immunogenic modulation in TRAMP-C2 cells. In vivo, enzalutamide mediated reduced genitourinary tissue weight, enlargement of the thymus, and increased levels of T-cell excision circles. Because no changes were seen in T-cell function, as determined by CD4+ T-cell proliferation and Treg functional assays, enzalutamide was determined to be immune inert. Enzalutamide did not diminish the Twist-vaccine’s ability to generate Twist-specific immunity. Twist was confirmed as a valid tumor antigen in TRAMP mice by immunohistochemistry. The combination of enzalutamide and Twist-vaccine resulted in significantly increased overall survival of TRAMP mice compared to other treatment groups (27.5 vs. 10.3 weeks). Notably, the effectiveness of the combination therapy increased with disease stage, i.e., the greatest survival benefit was seen in mice with advanced-stage prostate tumors. Conclusions These data support the combination of enzalutamide and immunotherapy as a promising treatment strategy for CRPC. PMID:24048332

Chronic low dose ethanol induces an aggressive metastatic phenotype in TRAMP mice, which is counteracted by parthenolide.

PubMed

Morel, Katherine L; Ormsby, Rebecca J; Solly, Emma L; Tran, Linh N K; Sweeney, Christopher J; Klebe, Sonja; Cordes, Nils; Sykes, Pamela J

2018-06-23

Despite advances in prostate cancer therapy, dissemination and growth of metastases results in shortened survival. Here we examined the potential anti-cancer effect of the NF-κB inhibitor parthenolide (PTL) and its water soluble analogue dimethylaminoparthenolide (DMAPT) on tumour progression and metastasis in the TRansgenic Adenocarcinoma of the Mouse Prostate (TRAMP) model of prostate cancer. Six-week-old male TRAMP mice received PTL (40 mg/kg in 10% ethanol/saline), DMAPT (100 mg/kg in sterile water), or vehicle controls by oral gavage thrice weekly until palpable tumour formation. DMAPT treatment slowed normal tumour development in TRAMP mice, extending the time-to-palpable prostate tumour by 20%. PTL did not slow overall tumour development, while the ethanol/saline vehicle used to administer PTL unexpectedly induced an aggressive metastatic tumour phenotype. Chronic ethanol/saline vehicle upregulated expression of NF-κB, MMP2, integrin β1, collagen IV, and laminin, and induced vascular basement membrane degradation in primary prostate tumours, as well as increased metastatic spread to the lung and liver. All of these changes were largely prevented by co-administration with PTL. DMAPT (in water) reduced metastasis to below that of water-control. These data suggest that DMAPT has the potential to be used as a cancer preventive and anti-metastatic therapy for prostate cancer. Although low levels of ethanol consumption have not been shown to strongly correlate with prostate cancer epidemiology, these results would support a potential effect of chronic low dose ethanol on metastasis and the TRAMP model provides a useful system in which to further explore the mechanisms involved.

High animal fat intake enhances prostate cancer progression and reduces glutathione peroxidase 3 expression in early stages of TRAMP mice.

PubMed

Chang, Seo-Na; Han, Juhee; Abdelkader, Tamer Said; Kim, Tae-Hyoun; Lee, Ji Min; Song, Juha; Kim, Kyung-Sul; Park, Jong-Hwan; Park, Jae-Hak

2014-09-01

Prostate cancer is the most frequently diagnosed cancer in Western men, and more men have been diagnosed at younger ages in recent years. A high-fat Western-style diet is a known risk factor for prostate cancer and increases oxidative stress. We evaluated the association between dietary animal fat and expression of antioxidant enzymes, particularly glutathione peroxidase 3 (GPx3), in the early stages of transgenic adenocarcinoma of the mouse prostate (TRAMP) mice. Six-week-old male nontransgenic and TRAMP mice were placed on high animal fat (45% Kcal fat) or control (10% Kcal fat) diets and sacrificed after 5 or 10 weeks. The histopathological score increased with age and high-fat diet consumption. The histopathological scores in dorsal and lateral lobes increased in the 10-week high-fat diet group (6.2±0.2 and 6.2±0.4, respectively) versus the 10-week control diet group (5.3±0.3 and 5.2±0.2, respectively). GPx3 decreased both at the mRNA and protein levels in mouse prostate. GPx3 mRNA expression decreased (∼36.27% and ∼23.91%, respectively) in the anterior and dorsolateral prostate of TRAMP mice fed a high-fat diet compared to TRAMP mice fed a control diet. Cholesterol treatment increased PC-3 human prostate cancer cell proliferation, decreased GPx3 mRNA and protein levels, and increased H2 O2 levels in culture medium. Moreover, increasing GPx3 mRNA expression by troglitazone in PC-3 cells decreased cell proliferation and lowered H2 O2 levels. Dietary fat enhances prostate cancer progression, possibly by suppressing GPx3 expression and increasing proliferation of prostate intraepithelial neoplasia (PIN) epithelial cells. © 2014 Wiley Periodicals, Inc.

Role of the TGF-Beta 1 in the Prevention of Prostate Cancer

DTIC Science & Technology

2001-04-01

the prostate or seminal vesicles delayed tumor development in the TRAMP mice through autocrine and paracrine pathways. 14. SUBJECT TERMS 15. NUMBER OF...it imperative to develop prevention strategies against this disease. Modifications in environmental, dietary, endocrine, or genetic factors may play a...This hypothesis is being tested in TRAMP transgenic mice, which develop spontaneous prostate cancer with features similar to that of human prostate

Imaging Prostate Cancer (PCa) Phenotype and Evolution

DTIC Science & Technology

2016-10-01

inhibit growth of some but not all cell lines. 2. Keywords: Deferiprone, aconitase, metabolism, tricarboxylic acid cycle , magnetic resonance 3...TRAMP C2 and MycCaP cell proliferation, migration, and invasiveness. Determine if knockdown of m-acon and Deferiprone inhibit TCA cycle activity...migration and inhibits TCA cycle (metabolism). Similarly in vivo (Aim 2), we 6 Fig. 2: Effect of DFP on in vivo growth of MycCaP (left) and TRAMP C2

Iodine Uptake and Prostate Cancer in the TRAMP Mouse Model

PubMed Central

Olvera-Caltzontzin, Paloma; Delgado, Guadalupe; Aceves, Carmen; Anguiano, Brenda

2013-01-01

Iodine supplementation exerts antitumor effects in several types of cancer. Iodide (I−) and iodine (I2) reduce cell proliferation and induce apoptosis in human prostate cancer cells (LNCaP and DU-145). Both chemical species decrease tumor growth in athymic mice xenografted with DU-145 cells. The aim of this study was to analyze the uptake and effects of iodine in a preclinical model of prostate cancer (transgenic adenocarcinoma of the mouse prostate [TRAMP] mice/SV40-TAG antigens), which develops cancer by 12 wks of age. 125I− and 125I2 uptake was analyzed in prostates from wild-type and TRAMP mice of 12 and 24 wks in the presence of perchlorate (inhibitor of the Na+/I− symporter [NIS]). NIS expression was quantified by quantitative polymerase chain reaction (qPCR). Mice (6 wks old) were supplemented with 0.125 mg I− plus 0.062 mg I2/mouse/day for 12 or 24 wks. The weight of the genitourinary tract (GUT), the number of acini with lesions, cell proliferation (levels of proliferating cell nuclear antigen [PCNA] by immunohistochemistry), p53 and p21 expression (by qPCR) and apoptosis (relative amount of nucleosomes by enzyme-linked immunosorbent assay) were evaluated. In both age-groups, normal and tumoral prostates take up both forms of iodine, but only I− uptake was blocked by perchlorate. Iodine supplementation prevented the overexpression of NIS in the TRAMP mice, but had no effect on the GUT weight, cell phenotype, proliferation or apoptosis. In TRAMP mice, iodine increased p53 expression but had no effect on p21 (a p53-dependent gene). Our data corroborate NIS involvement in I− uptake and support the notion that another transporter mediates I2 uptake. Iodine did not prevent cancer progression. This result could be explained by a strong inactivation of the p53 pathway by TAG antigens. PMID:24306422

Iodine uptake and prostate cancer in the TRAMP mouse model.

PubMed

Olvera-Caltzontzin, Paloma; Delgado, Guadalupe; Aceves, Carmen; Anguiano, Brenda

2013-11-08

Iodine supplementation exerts antitumor effects in several types of cancer. Iodide (I⁻) and iodine (I₂) reduce cell proliferation and induce apoptosis in human prostate cancer cells (LNCaP and DU-145). Both chemical species decrease tumor growth in athymic mice xenografted with DU-145 cells. The aim of this study was to analyze the uptake and effects of iodine in a preclinical model of prostate cancer (transgenic adenocarcinoma of the mouse prostate [TRAMP] mice/SV40-TAG antigens), which develops cancer by 12 wks of age. ¹²⁵I⁻ and ¹²⁵I₂ uptake was analyzed in prostates from wild-type and TRAMP mice of 12 and 24 wks in the presence of perchlorate (inhibitor of the Na⁺/I⁻ symporter [NIS]). NIS expression was quantified by quantitative polymerase chain reaction (qPCR). Mice (6 wks old) were supplemented with 0.125 mg I⁻ plus 0.062 mg I₂/mouse/day for 12 or 24 wks. The weight of the genitourinary tract (GUT), the number of acini with lesions, cell proliferation (levels of proliferating cell nuclear antigen [PCNA] by immunohistochemistry), p53 and p21 expression (by qPCR) and apoptosis (relative amount of nucleosomes by enzyme-linked immunosorbent assay) were evaluated. In both age-groups, normal and tumoral prostates take up both forms of iodine, but only I⁻ uptake was blocked by perchlorate. Iodine supplementation prevented the overexpression of NIS in the TRAMP mice, but had no effect on the GUT weight, cell phenotype, proliferation or apoptosis. In TRAMP mice, iodine increased p53 expression but had no effect on p21 (a p53-dependent gene). Our data corroborate NIS involvement in I⁻ uptake and support the notion that another transporter mediates I₂ uptake. Iodine did not prevent cancer progression. This result could be explained by a strong inactivation of the p53 pathway by TAG antigens.

Dietary tomato and lycopene impact androgen signaling- and carcinogenesis-related gene expression during early TRAMP prostate carcinogenesis

PubMed Central

Wan, Lei; Tan, Hsueh-Li; Thomas-Ahner, Jennifer M.; Pearl, Dennis K.; Erdman, John W.; Moran, Nancy E.; Clinton, Steven K.

2014-01-01

Consumption of tomato products containing the carotenoid lycopene is associated with a reduced risk of prostate cancer. To identify gene expression patterns associated with early testosterone-driven prostate carcinogenesis, which are impacted by dietary tomato and lycopene, wild type (WT) and transgenic adenocarcinoma of the mouse prostate (TRAMP) mice were fed control or tomato- or lycopene-containing diets from 4-10 wk-of-age. Eight-week-old mice underwent sham surgery, castration, or castration followed by testosterone-repletion (2.5 mg/kg/d initiated 1 wk after castration). Ten-wk-old intact TRAMP mice exhibit early multifocal prostatic intraepithelial neoplasia (PIN). Of the 200 prostate cancer-related genes measured by quantitative NanoString®, 189 are detectable, 164 significantly differ by genotype, 179 by testosterone status, and 30 by diet type (P<0.05). In TRAMP, expression of Birc5, Mki67, Aurkb, Ccnb2, Foxm1, and Ccne2 is greater compared to WT and is decreased by castration. In parallel, castration reduces Ki67-positive staining (P<0.0001) compared to intact and testosterone-repleted TRAMP mice. Expression of genes involved in androgen metabolism/signaling pathways are reduced by lycopene feeding (Srd5a1) and by tomato-feeding (Srd5a2, Pxn, and Srebf1). Additionally, tomato-feeding significantly reduced expression of genes associated with stem cell features, Aldh1a and Ly6a, while lycopene-feeding significantly reduced expression of neuroendocrine differentiation-related genes, Ngfr and Syp. Collectively, these studies demonstrate a profile of testosterone-regulated genes associated with early stages of prostate carcinogenesis that are potential mechanistic targets of dietary tomato components. Future studies on androgen signaling/metabolism, stem cell features, and neuroendocrine differentiation pathways may elucidate the mechanisms by which dietary tomato and lycopene impact prostate cancer risk. PMID:25315431

Novel Immune-Modulating Cellular Vaccine for Prostate Cancer Immunotherapy

DTIC Science & Technology

2015-10-01

the 3-month timeline and details provided in year 1 report. Anti-murine CTLA4 RNA Murine PAP Murine PSMA Murine PAP no signal sequence (mPAP-SS...Murine PSMA no signal sequence (mPSMA-SS) Year 2 (current) report: In addition to what we proposed to do, we wanted to determine if other...PAP, PSCA, PSMA and STEAP in the prostate cell lines TRAMP-C1 and TRAMP-C2. Both are cell lines that have been generated by in vitro propagation of

When invasive ants meet: effects of outbreeding on queen performance in the tramp ant Cardiocondyla itsukii.

PubMed

Heinze, Jürgen; Frohschammer, Sabine; Bernadou, Abel

2017-08-18

Most disturbed habitats in the tropics and subtropics harbor numerous species of invasive ants, and occasionally the same species has been introduced repeatedly from multiple geographical sources. We examined how experimental crossbreeding between sexuals from different populations affects the fitness of queens of the tramp ant Cardiocondyla itsukii, which is widely distributed in Asia and the Pacific Islands. Eggs laid by queens that mated with nestmate males had a higher hatching rate than eggs laid by queens mated to males from neighboring (Hawaii × Kauai) or distant introduced populations (Hawaii/Kauai × Okinawa). Furthermore, inbreeding queens had a longer lifespan and produced a less female-biased offspring sex ratio than queens from allopatric mating. This suggests that the genetic divergence between different source populations may already be so large that in case of multiple invasions eventual crossbreeding might negatively affect the fitness of tramp ants. © 2017 Institute of Zoology, Chinese Academy of Sciences.

Hyperthermic treatment at 56 °C induces tumour-specific immune protection in a mouse model of prostate cancer in both prophylactic and therapeutic immunization regimens.

PubMed

De Sanctis, Francesco; Sandri, Sara; Martini, Matteo; Mazzocco, Marta; Fiore, Alessandra; Trovato, Rosalinda; Garetto, Stefano; Brusa, Davide; Ugel, Stefano; Sartoris, Silvia

2018-06-14

Most active cancer immunotherapies able to induce a long-lasting protection against tumours are based on the activation of tumour-specific cytotoxic T lymphocytes (CTLs). Cell death by hyperthermia induces apoptosis followed by secondary necrosis, with the production of factors named "danger associated molecular pattern" (DAMP) molecules (DAMPs), that activate dendritic cells (DCs) to perform antigen uptake, processing and presentation, followed by CTLs cross priming. In many published studies, hyperthermia treatment of tumour cells is performed at 42-45 °C; these temperatures mainly promote cell surface expression of DAMPs. Treatment at 56 °C of tumour cells was shown to induce DAMPs secretion rather than their cell surface expression, improving DC activation and CTL cross priming in vitro. Thus we tested the relevance of this finding in vivo on the generation of a tumour-specific memory immune response, in the TRAMP-C2 mouse prostate carcinoma transplantable model. TRAMP-C2 tumour cells treated at 56 °C were able not only to activate DCs in vitro but also to trigger a tumour-specific CTL-dependent immune response in vivo. Prophylactic vaccination with 56 °C-treated TRAMP-C2 tumour cells alone provided protection against TRAMP-C2 tumour growth in vivo, whilst in the therapeutic regimen, control of tumour growth was achieved combining immunization with adjuvant chemotherapy. Copyright © 2018 Elsevier Ltd. All rights reserved.

Gene signatures distinguish stage-specific prostate cancer stem cells isolated from transgenic adenocarcinoma of the mouse prostate lesions and predict the malignancy of human tumors.

PubMed

Mazzoleni, Stefania; Jachetti, Elena; Morosini, Sara; Grioni, Matteo; Piras, Ignazio Stefano; Pala, Mauro; Bulfone, Alessandro; Freschi, Massimo; Bellone, Matteo; Galli, Rossella

2013-09-01

The relevant social and economic impact of prostate adenocarcinoma, one of the leading causes of death in men, urges critical improvements in knowledge of the pathogenesis and cure of this disease. These can also be achieved by implementing in vitro and in vivo preclinical models by taking advantage of prostate cancer stem cells (PCSCs). The best-characterized mouse model of prostate cancer is the transgenic adenocarcinoma of the mouse prostate (TRAMP) model. TRAMP mice develop a progressive lesion called prostatic intraepithelial neoplasia that evolves into adenocarcinoma (AD) between 24 and 30 weeks of age. ADs often metastasize to lymph nodes, lung, bones, and kidneys. Eventually, approximately 5% of the mice develop an androgen-independent neuroendocrine adenocarcinoma. Here we report the establishment of long-term self-renewing PCSC lines from the different stages of TRAMP progression by application of the neurosphere assay. Stage-specific prostate cell lines were endowed with the critical features expected from malignant bona fide cancer stem cells, namely, self-renewal, multipotency, and tumorigenicity. Notably, transcriptome analysis of stage-specific PCSCs resulted in the generation of well-defined, meaningful gene signatures, which identify distinct stages of human tumor progression. As such, TRAMP-derived PCSCs represent a novel and valuable preclinical model for elucidating the pathogenetic mechanisms leading to prostate adenocarcinoma and for the identification of molecular mediators to be pursued as therapeutic targets.

The p202 Gene as a Tumor Suppressor in Prostate Cancer Cells

DTIC Science & Technology

2005-06-01

Inst 29. Shibata MA, Ward JM, Devor DE , Liu ML, Green JE. 39. Bagatell R, Khan 0, Paine-Murrieta G, et al. Destabi- 2000;92:1918-25. Progression of...search for treatment options [7, 71]. Therefore, in this study, we will use the autochthonous TRAMP (transgenic adenocarcinoma of murine prostate... adenocarcinoma at 16 weeks of age. Mature 10-and 16-week-old EZC-TRAMP mice (n=5 per group) will be i.v. injected with CMV-BikDD/SN, ATTP-BikDD/SN, ARR2PB

Epigenetics Reactivation of Nrf2 in Prostate TRAMP C1 Cells by Curcumin Analogue FN1.

PubMed

Li, Wenji; Pung, Doug; Su, Zheng-Yuan; Guo, Yue; Zhang, Chengyue; Yang, Anne Yuqing; Zheng, Xi; Du, Zhi-Yun; Zhang, Kun; Kong, Ah-Ng

2016-04-18

It has previously been shown that curcumin can effectively inhibit prostate cancer proliferation and progression in TRAMP mice, potentially acting through the hypomethylation of the Nrf2 gene promoter and hence activation of the Nrf2 pathway to enhance cell antioxidative defense. FN1 is a synthetic curcumin analogue that shows stronger anticancer activity than curcumin in other reports. We aimed to explore the epigenetic modification of FN1 that restores Nrf2 expression in TRAMP-C1 cells. Stably transfected HepG2-C8 cells were used to investigate the effect of FN1 on the Nrf2- antioxidant response element (ARE) pathway. Real-time quantitative PCR and Western blotting were applied to study the influence of FN1 on endogenous Nrf2 and its downstream genes. Bisulfite genomic sequencing (BGS) and methylated DNA immunoprecipitation (MeDIP) were then performed to examine the methylation profile of the Nrf2 promoter. An anchorage-independent colony-formation analysis was conducted to examine the tumor inhibition activity of FN1. Epigenetic modification enzymes, including DNMTs and HDACs, were investigated by Western blotting. The luciferase reporter assay indicated that FN1 was more potent than curcumin in activating the Nrf2-ARE pathway. FN1 increased the expression of Nrf2 and its downstream detoxifying enzymes. FN1 significantly inhibited the colony formation of TRAMP-C1 cells. BGS and MeDIP assays revealed that FN1 treatment (250 nM for 3 days) reduced the percentage of CpG methylation of the Nrf2 promoter. FN1 also downregulated epigenetic modification enzymes. In conclusion, our results suggest that FN1 is a novel anticancer agent for prostate cancer. In the TRAMP-C1 cell line, FN1 can increase the level of Nrf2 and downstream genes via activating the Nrf2-ARE pathway and inhibit the colony formation potentially through the decreased expression of keap1 coupled with CpG demethylation of the Nrf2 promoter. This CpG demethylation effect may come from decreased epigenetic modification enzymes, such as DNMT1, DNMT3a, DNMT3b, and HDAC4.

The interactions of dietary tomato powder and soy germ on prostate carcinogenesis in the TRAMP model

PubMed Central

Zuniga, Krystle E.; Clinton, Steven K; Erdman, John W.

2013-01-01

The interactions between bioactive rich food components within a complex human diet for the inhibition of prostate carcinogenesis (PCa) are largely unknown and difficult to quantify in humans. Tomato and soy products have each shown anti-PCa activity in laboratory studies. The objective of this study was to determine the efficacy of dietary tomato and soy germ, alone and in combination, for the inhibition of prostate carcinogenesis in the transgenic adenocarcinoma of the mouse prostate (TRAMP) model. At 4 weeks of age, male C57BL/6 × FVB TRAMP mice (n=119) were randomized to consume: AIN-93G control, 10% whole tomato powder (TP), 2% soy germ powder (SG) or 10% tomato powder with 2% soy germ powder (TP+SG) for 14 weeks. 100% of mice fed the control diet had PCa, while PCa incidence was significantly lower in mice consuming TP (61%, p<0.001), SG (66%, p<0.001) and TP+SG (45%, p<0.001). Although the protection offered by the combination of TP and SG was not synergistic, it was the most effective intervention. TP, SG and TP+SG increased apoptotic index (AI) and modestly reduced the proliferative index (PI) in the prostate epithelium of TRAMP mice exhibiting primarily prostatic intraepithelial neoplasia. The dramatic reduction in the PI/AI ratio by the dietary interventions suggests that the control mice experience a stronger stimulus for malignant progression in the prostate microenvironment. Maximally effective and safe strategies for PCa prevention may result from optimizing combinations of nutrients and bioactives through an orchestration of dietary patterns. PMID:23592738

Observations of Radical Precursors during TexAQS II: Findings and Implications

NASA Astrophysics Data System (ADS)

Olaguer, E. P.; Lefer, B. L.; Rappenglueck, B.; Pinto, J. P.

2009-12-01

The Texas Environmental Research Consortium (TERC) sponsored and helped organize significant components of the Second Texas Air Quality Study (TexAQS II). Some of the TERC-sponsored experiments, most notably those associated with the TexAQS II Radical and Aerosol Measurement Project (TRAMP) sited on top of the Moody Tower at the University of Houston, found evidence for the importance of short-lived radical sources such as formaldehyde (HCHO) and nitrous acid (HONO) in increasing ozone productivity. During TRAMP, daytime HCHO pulses as large as 32 ppb were observed and attributed to industrial activities upwind in the Houston Ship Channel (HSC), and HCHO peaks as large as 52 ppb were detected by in-situ surface monitors in the HSC. In addition, an instrumented Piper Aztec aircraft observed plumes of apparent primary formaldehyde in flares from petrochemical facilities in the HSC. In one such combustion plume, depleted of ozone by large NOx emissions, the Piper Aztec measured an HCHO-to-CO ratio three times that of mobile sources. HCHO from uncounted primary sources or ozonolysis of underestimated olefin emissions could significantly increase ozone productivity in Houston beyond previous expectations. Simulations with the CAMx model show that additional emissions of HCHO from industrial flares can increase peak ozone in Houston by up to 30 ppb, depending on conditions in the planetary boundary layer. Other findings from TexAQS II include significant concentrations of HONO throughout the day, well in excess of current air quality model predictions, with large nocturnal vertical gradients indicating a surface or near-surface source of HONO, and large concentrations of night-time radicals (~30 ppt HO2). Additional HONO sources could increase daytime ozone by more than 10 ppb. Improving the representation of primary and secondary HCHO and HONO in air quality models could enhance the effectiveness of simulated control strategies, and thus make ozone attainment demonstrations easier. (This abstract does not necessarily reflect EPA policy.)

Chemopreventive effect of Korean Angelica root extract on TRAMP carcinogenesis and integrative “omic” profiling of affected neuroendocrine carcinomas

PubMed Central

Zhang, Jinhui; Wang, Lei; Zhang, Yong; Li, Li; Tang, Suni; Xing, Chengguo; Kim, Sung-Hoon; Jiang, Cheng; Lü, Junxuan

2016-01-01

Angelica gigas Nakai (AGN) root ethanol extract exerts anti-cancer activity in several allograft and xenograft models. Here we examined its chemopreventive efficacy through gavage administration against primary carcinogenesis in the transgenic adenocarcinoma of mouse prostate (TRAMP) model. Male C57BL/6 TRAMP mice and wild type littermates were given a daily gavage (5 mg/mouse, Monday-Friday) of AGN or vehicle, beginning at 8 weeks of age (WOA). All mice were terminated at 24 WOA, unless earlier euthanasia was necessitated by large tumors. Whereas AGN-treated TRAMP mice decreased dorsolateral prostate lesion growth by 30% (P = 0.009), they developed fewer and smaller neuroendocrine-carcinomas (NE-Ca) (0.12 g/mouse) than vehicle-treated counterparts (0.81g/mouse, P = 0.037). We analyzed the proteome and transcriptome of banked NE-Ca to gain molecular insights. Angiogenesis-antibody array detected a substantial reduction in AGN-treated NE-Ca of basic fibroblast growth factor (FGF2), an angiogenesis stimulator. iTRAQ proteomics plus data mining suggested changes of genes upstream and downstream of FGF2 functionally consistent with AGN inhibiting FGF2/FGFR1 signaling at different levels of the transduction cascade. Moreover, AGN upregulated mRNA of genes related to immune responses, restored expression of many tumor suppressor genes, and prostate function and muscle differentiation genes. On the other hand, AGN down-regulated mRNA of genes related to neuron signaling, oncofetal antigens, inflammation and mast cells, Wnt signaling, embryonic morphogenesis, biosynthesis, cell adhesion, motility, invasion and angiogenesis. These changes suggest not only multiple cancer cell targeting actions of AGN but also impact on the tumor microenvironments such as angiogenesis, inflammation and immune surveillance. PMID:25307620

Macrophages From Irradiated Tumors Express Higher Levels of iNOS, Arginase-I and COX-2, and Promote Tumor Growth

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tsai, C.-S.; Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taiwan; Chen, F.-H.

2007-06-01

Purpose: To investigate the effects of single and fractionated doses of radiation on tumors and tumor-associated macrophages (TAMs), and to elucidate the potential of TAMs to influence tumor growth. Methods and Materials: A murine prostate cell line, TRAMP-C1, was grown in C57Bl/6J mice to 4-mm tumor diameter and irradiated with either 25 Gy in a single dose, or 60 Gy in 15 fractions. The tumors were removed at the indicated times and assessed for a variety of markers related to TAM content, activation status, and function. Results: In tumors receiving a single radiation dose, arginase (Arg-I), and cycloxygenase-2 (COX-2) mRNAmore » expression increased as a small transient wave within 24 h and a larger persistent wave starting after 3 days. Inducible nitric oxide synthase (iNOS) mRNA was elevated only after 3 days and continued to increase up to 3 weeks. After fractionated irradiation, Arg-1 and COX-2 mRNA levels increased within 5 days, whereas iNOS was increased only after 10 fractions of irradiation had been given. Increased levels of Arg-I, COX-2, and, to a lesser extent, iNOS protein were found to associate with TAMs 1-2 weeks after tumor irradiation. Function of TAMs were compared by mixing them with TRAMP-C1 cells and injecting them into mice; TRAMP-C1 cells mixed with TAMs from irradiated tumors appeared earlier and grew significantly faster than those mixed with TAMs from unirradiated tumors or TRAMP-C1 alone. Conclusions: Tumor-associated macrophages in the postirradiated tumor microenvironment express higher levels of Arg-1, COX-2, and iNOS, and promote early tumor growth in vivo.« less

"The Tramp", a blood donation propagandist?

PubMed

Lefrère, J-J; Garraud, O

2016-02-01

The French pioneer for blood transfusion, who eventually organized the very early blood transfusion centers worldwide, went to imagine a scenario written in purpose for Charlie Chaplin, the unique character of "The Tramp" ("Charlot" in French). The movie Star was offered to feature a blood donation propagandist, and no longer the perpetual, well-known, "loser". This anecdote, besides being amusing, tells a lot on how Arnault Tzank encompassed all the difficulties in collecting blood enough to meet the demand, at all times; his proposal turns out to be extremely modern and questions nowadays marketing for blood donation. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

Regeneration of the gut requires retinoic acid in the budding ascidian Polyandrocarpa misakiensis.

PubMed

Kaneko, Nagamoto; Katsuyama, You; Kawamura, Kazuo; Fujiwara, Shigeki

2010-06-01

The protochordate ascidian Polyandrocarpa misakiensis has a striking ability to regenerate. When the posterior half of the adult body is amputated, the anterior half completely loses the esophagus, stomach and intestine. These organs are reconstituted in a week. Histological observation revealed that the regeneration involves transdifferentiation of the atrial epithelium near the cut surface. The morphological features of the gut primordium were similar to those observed in the developing bud of this species. Inhibitors of the synthesis of retinoic acid (RA) suppressed the formation of the gut. 13-cis RA rescued the regenerates from the inhibitor-induced hypoplasia. These results suggest that RA is required for the regeneration of the gut. A gene encoding the RA receptor (Pm-RAR) and its target gene, TRAMP, were expressed in and around the regenerating gut. Pm-RAR-specific and TRAMP-specific double-stranded RNA molecules inhibited the regeneration of the gut, indicating that the RA signal is mediated at least in part by Pm-RAR and TRAMP. These results suggested that RA triggers the transdifferentiation of the atrial epithelium into the gut in regenerating animals, as it does during asexual reproduction.

Method for treating rare earth-transition metal scrap

DOEpatents

Schmidt, Frederick A.; Peterson, David T.; Wheelock, John T.; Jones, Lawrence L.

1992-12-29

Rare earth-transition metal (e.g., iron) scrap (e.g., Nd-Fe-B scrap) is flux (slag) remelted to reduce tramp non-metallic impurities, such as oxygen and nitrogen, and metallic impurities, such as Li, Na, Al, etc., picked up by the scrap from previous fabrication operations. The tramp impurities are reduced to concentrations acceptable for reuse of the treated alloy in the manufacture of end-use articles, such as permanent magnets. The scrap is electroslag or inductoslag melted using a prefused, rare earth fluoride-bearing flux of CaF.sub.2, CaCl.sub.2 or mixtures thereof or the slag resulting from practice of the thermite reduction process to make a rare earth-iron alloy.

Method for treating rare earth-transition metal scrap

DOEpatents

Schmidt, F.A.; Peterson, D.T.; Wheelock, J.T.; Jones, L.L.

1992-12-29

Rare earth-transition metal (e.g., iron) scrap (e.g., Nd-Fe-B scrap) is flux (slag) remelted to reduce tramp non-metallic impurities, such as oxygen and nitrogen, and metallic impurities, such as Li, Na, Al, etc., picked up by the scrap from previous fabrication operations. The tramp impurities are reduced to concentrations acceptable for reuse of the treated alloy in the manufacture of end-use articles, such as permanent magnets. The scrap is electroslag or inductoslag melted using a rare earth fluoride-bearing flux of CaF[sub 2], CaCl[sub 2] or mixtures thereof or the slag resulting from practice of the thermite reduction process to make a rare earth-iron alloy. 3 figs.

Evaluation of the cancer chemopreventive efficacy of rice bran in genetic mouse models of breast, prostate and intestinal carcinogenesis.

PubMed

Verschoyle, R D; Greaves, P; Cai, H; Edwards, R E; Steward, W P; Gescher, A J

2007-01-29

Brown rice is a staple dietary constituent in Asia, whereas rice consumed in the Western world is generally white, obtained from brown rice by removal of the bran. We tested the hypothesis that rice bran interferes with development of tumours in TAg, TRansgenic Adenocarcinoma of the Mouse Prostate (TRAMP) or Apc(Min) mice, genetic models of mammary, prostate and intestinal carcinogenesis, respectively. Mice received rice bran (30%) in AIN-93G diet throughout their post-weaning lifespan. In TAg and TRAMP mice, rice bran did not affect carcinoma development. In TRAMP or wild-type C57Bl6/J mice, dietary rice bran increased kidney weight by 18 and 20%, respectively. Consumption of rice bran reduced numbers of intestinal adenomas in Apc(Min) mice by 51% (P<0.01), compared to mice on control diet. In parallel, dietary rice bran decreased intestinal haemorrhage in these mice, as reflected by increased haematocrit. At 10% in the diet, rice bran did not significantly retard Apc(Min) adenoma development. Likewise, low-fibre rice bran (30% in the diet) did not affect intestinal carcinogenesis, suggesting that the fibrous constituents of the bran mediate chemopreventive efficacy. The results suggest that rice bran might be beneficially evaluated as a putative chemopreventive intervention in humans with intestinal polyps.

Serum antibodies to huntingtin interacting protein-1: a new blood test for prostate cancer.

PubMed

Bradley, Sarah V; Oravecz-Wilson, Katherine I; Bougeard, Gaelle; Mizukami, Ikuko; Li, Lina; Munaco, Anthony J; Sreekumar, Arun; Corradetti, Michael N; Chinnaiyan, Arul M; Sanda, Martin G; Ross, Theodora S

2005-05-15

Huntingtin-interacting protein 1 (HIP1) is frequently overexpressed in prostate cancer. HIP1 is a clathrin-binding protein involved in growth factor receptor trafficking that transforms fibroblasts by prolonging the half-life of growth factor receptors. In addition to human cancers, HIP1 is also overexpressed in prostate tumors from the transgenic adenocarcinoma of the mouse prostate (TRAMP) mouse model. Here we provide evidence that HIP1 plays an important role in mouse tumor development, as tumor formation in the TRAMP mice was impaired in the Hip1null/null background. In addition, we report that autoantibodies to HIP1 developed in the sera of TRAMP mice with prostate cancer as well as in the sera from human prostate cancer patients. This led to the development of an anti-HIP1 serum test in humans that had a similar sensitivity and specificity to the anti-alpha-methylacyl CoA racemase (AMACR) and prostate-specific antigen tests for prostate cancer and when combined with the anti-AMACR test yielded a specificity of 97%. These data suggest that HIP1 plays a functional role in tumorigenesis and that a positive HIP1 autoantibody test may be an important serum marker of prostate cancer.

Prostate cancer-derived cathelicidin-related antimicrobial peptide facilitates macrophage differentiation and polarization of immature myeloid progenitors to protumorigenic macrophages.

PubMed

Cha, Ha-Ram; Lee, Joo Hyoung; Hensel, Jonathan A; Sawant, Anandi B; Davis, Brittney H; Lee, Carnellia M; Deshane, Jessy S; Ponnazhagan, Selvarangan

2016-05-01

A growing body of evidence indicates a positive correlation between expression of human antimicrobial peptide leucin leucin 37 (LL-37) and progression of epithelial cancers, including prostate cancer (PCa). Although the molecular mechanisms for this correlation has not yet been elucidated, the primary function of LL-37 as a chemotactic molecule for innate immune effector cells suggests its possible association in coordinating protumorigenic mechanisms, mediated by tumor-infiltrating immune cells. To investigate protumorigenic role(s) of cathelicidin-related antimicrobial peptide (CRAMP), a murine orthologue of LL-37, the present study compared tumor growth kinetics between mouse PCa cell lines with and without CRAMP expression (TRAMP-C1 and TRAMP-C1(CRAMP-sh) , respectively) in immunocompetent mice. CRAMP-mediated chemotaxis of different innate immune cell types to the tumor microenvironment (TME) was observed in vivo and confirmed by in vitro chemotaxis assay. The role of CRAMP in differentiation and polarization of immature myeloid progenitors (IMPs) to protumorigenic type 2 macrophages (M2) in TME was determined by adoptive transfer of IMPs into mice bearing CRAMP(+) and CRAMP(-) tumors. To differentiate protumorigenic events mediated by tumor-derived CRAMP from host immune cell-derived CRAMP, tumor challenge study was performed in CRAMP-deficient mice. To identify mechanisms of CRAMP function, macrophage colony stimulating factor (M-CSF) and monocyte chemoattractant protein 1 (MCP-1) gene expression was analyzed by QRT-PCR and STAT3 signaling was determined by immunoblotting. Significantly delayed tumor growth was observed in wild-type (WT) mice implanted with TRAMP-C1(CRAMP-sh) cells compared to mice implanted with TRAMP-C1 cells. CRAMP(+) TME induced increased number of IMP differentiation into protumorigenic M2 macrophages compared to CRAMP(-) TME, indicating tumor-derived CRAMP facilitates differentiation and polarization of IMPs toward M2. Tumor challenge study in CRAMP deficient mice showed comparable tumor growth kinetics with WT mice, suggesting tumor-derived CRAMP plays a crucial role in PCa progression. In vitro study demonstrated that overexpressed M-CSF and MCP-1 in TRAMP-C1 cells through CRAMP-mediated autocrine signaling, involving p65, regulates IMP-to-M2 differentiation/polarization through STAT3 activation. Altogether, the present study suggests that overexpressed CRAMP in prostate tumor initially chemoattracts IMPs to TME and mediates differentiation and polarization of early myeloid progenitors into protumorigenic M2 macrophages during PCa progression. Thus, selective downregulation of CRAMP in tumor cells in situ may benefit overcoming immunosuppressive mechanisms in PCa. © 2016 Wiley Periodicals, Inc.

STS-69 Flight Day 9 Video File

NASA Technical Reports Server (NTRS)

1995-01-01

The song, 'He's A Tramp', from the Walt Disney cartoon movie, 'Lady and the Tramp', awakened the astronauts, Cmdr. Dave Walker, Pilot Ken Cockrell, and Mission Specialists Jim Voss, Jim Newman, and Mike Gernhardt, on the ninth day of the STS-69 mission. The Wake Shield Facility (WSF) was again unberthed from the shuttle cargo bay and , using the shuttle's robot arm, held over the side of the shuttle for five hours where it collected data on the electrical field build-up around the spacecraft as part of the Charging Hazards and Wake Studies Experiment (CHAWS). Voss and Gernhardt rehearsed their Extravehicular Activity (EVA) spacewalk, which was planned for the next day. Earth views included cloud cover, a hurricane, and its eye.

STS-69 flight day 9 highlights

NASA Astrophysics Data System (ADS)

1995-09-01

The song, 'He's A Tramp', from the Walt Disney cartoon movie, 'Lady and the Tramp', awakened the astronauts, Cmdr. Dave Walker, Pilot Ken Cockrell, and Mission Specialists Jim Voss, Jim Newman, and Mike Gernhardt, on the ninth day of the STS-69 mission. The Wake Shield Facility (WSF) was again unberthed from the shuttle cargo bay and , using the shuttle's robot arm, held over the side of the shuttle for five hours where it collected data on the electrical field build-up around the spacecraft as part of the Charging Hazards and Wake Studies Experiment (CHAWS). Voss and Gernhardt rehearsed their Extravehicular Activity (EVA) spacewalk, which was planned for the next day. Earth views included cloud cover, a hurricane, and its eye.

Leupaxin acts as a mediator in prostate carcinoma progression through deregulation of p120catenin expression.

PubMed

Kaulfuss, S; von Hardenberg, S; Schweyer, S; Herr, A M; Laccone, F; Wolf, S; Burfeind, P

2009-11-12

Recently, we could show that the focal adhesion protein leupaxin (LPXN) is expressed in human prostate carcinomas (PCa) and induces invasiveness of androgen-independent PCa cells. In this study we show that LPXN enhanced the progression of existing PCa in vivo by breeding transgenic mice with prostate-specific LPXN expression and TRAMP mice (transgenic adenocarcinoma of mouse prostate). Double transgenic LPXN/TRAMP mice showed a significant increase in poorly differentiated PCa and distant metastases as compared with control TRAMP mice. Additional studies on primary PCa cells generated from both transgenic backgrounds confirmed the connection regarding LPXN overexpression and increased motility and invasiveness of PCa cells. One mediator of LPXN-induced invasion was found to be the cell-cell adhesion protein p120catenin (p120CTN). Both in vitro and in vivo experiments revealed that p120CTN expression negatively correlates with LPXN expression, followed by a redistribution of beta-catenin. Downregulation of LPXN using small interfering RNAs (siRNAs) resulted in a membranous localization of beta-catenin, whereas strong nuclear accumulation of beta-catenin was observed in p120CTN knockdown cells leading to enhanced transcription of the beta-catenin target gene matrix metalloprotease-7. In conclusion, the present results indicate that LPXN enhances the progression of PCa through downregulation of p120CTN expression and that LPXN could function as a marker for aggressive PCa in the future.

Evaluation of the cancer chemopreventive efficacy of rice bran in genetic mouse models of breast, prostate and intestinal carcinogenesis

PubMed Central

Verschoyle, R D; Greaves, P; Cai, H; Edwards, R E; Steward, W P; Gescher, A J

2007-01-01

Brown rice is a staple dietary constituent in Asia, whereas rice consumed in the Western world is generally white, obtained from brown rice by removal of the bran. We tested the hypothesis that rice bran interferes with development of tumours in TAg, TRansgenic Adenocarcinoma of the Mouse Prostate (TRAMP) or ApcMin mice, genetic models of mammary, prostate and intestinal carcinogenesis, respectively. Mice received rice bran (30%) in AIN-93G diet throughout their post-weaning lifespan. In TAg and TRAMP mice, rice bran did not affect carcinoma development. In TRAMP or wild-type C57Bl6/J mice, dietary rice bran increased kidney weight by 18 and 20%, respectively. Consumption of rice bran reduced numbers of intestinal adenomas in ApcMin mice by 51% (P<0.01), compared to mice on control diet. In parallel, dietary rice bran decreased intestinal haemorrhage in these mice, as reflected by increased haematocrit. At 10% in the diet, rice bran did not significantly retard ApcMin adenoma development. Likewise, low-fibre rice bran (30% in the diet) did not affect intestinal carcinogenesis, suggesting that the fibrous constituents of the bran mediate chemopreventive efficacy. The results suggest that rice bran might be beneficially evaluated as a putative chemopreventive intervention in humans with intestinal polyps. PMID:17211473

The Landscape of Somatic Chromosomal Copy Number Aberrations in GEM Models of Prostate Carcinoma

PubMed Central

Bianchi-Frias, Daniella; Hernandez, Susana A.; Coleman, Roger; Wu, Hong; Nelson, Peter S.

2015-01-01

Human prostate cancer (PCa) is known to harbor recurrent genomic aberrations consisting of chromosomal losses, gains, rearrangements and mutations that involve oncogenes and tumor suppressors. Genetically engineered mouse (GEM) models have been constructed to assess the causal role of these putative oncogenic events and provide molecular insight into disease pathogenesis. While GEM models generally initiate neoplasia by manipulating a single gene, expression profiles of GEM tumors typically comprise hundreds of transcript alterations. It is unclear whether these transcriptional changes represent the pleiotropic effects of single oncogenes, and/or cooperating genomic or epigenomic events. Therefore, it was determined if structural chromosomal alterations occur in GEM models of PCa and whether the changes are concordant with human carcinomas. Whole genome array-based comparative genomic hybridization (CGH) was used to identify somatic chromosomal copy number aberrations (SCNAs) in the widely used TRAMP, Hi-Myc, Pten-null and LADY GEM models. Interestingly, very few SCNAs were identified and the genomic architecture of Hi-Myc, Pten-null and LADY tumors were essentially identical to the germline. TRAMP neuroendocrine carcinomas contained SCNAs, which comprised three recurrent aberrations including a single copy loss of chromosome 19 (encoding Pten). In contrast, cell lines derived from the TRAMP, Hi-Myc, and Pten-null tumors were notable for numerous SCNAs that included copy gains of chromosome 15 (encoding Myc) and losses of chromosome 11 (encoding p53). PMID:25298407

Effects of dietary saw palmetto on the prostate of transgenic adenocarcinoma of the mouse prostate model (TRAMP).

PubMed

Wadsworth, Teri L; Worstell, Teresa R; Greenberg, Norman M; Roselli, Charles E

2007-05-01

Several of the proposed mechanisms for the actions of the liposterolic extract of saw palmetto (SPE) are exerted on known risk factors for prostate cancer (CaP). This study investigated whether SPE could prevent the progression of CaP in a transgenic adenocarcinoma of the mouse prostate (TRAMP) model. Two different doses of SPE designed to deliver 50 mg/kg/day SPE and 300 mg/kg/day SPE were administered in a custom diet to TRAMP mice for 12 or 24 weeks. Body and organ weights were used to evaluate toxicity, and radioimmunoassay was used to measure plasma and tissue androgen levels to monitor effects of SPE on 5alpha reductase activity. Prostate tissues were evaluated histologically to determine the effect of treatment on tumor grade, cell proliferation, and apoptosis. Treatment with 300 mg/kg/day SPE from 4 to 24 weeks of age significantly reduced the concentration of 5alpha-dihydrotestosterone (DHT) in the prostate and resulted in a significant increase in apoptosis and significant decrease in pathological tumor grade and frank tumor incidence. Dietary supplementation with SPE may be effective in controlling CaP tumorigenesis. SPE suppression of prostatic DHT levels lends support to the hypothesis that inhibition of the enzyme 5alpha-reductase is a mechanism of action of this substance. (c) 2007 Wiley-Liss, Inc.

Suppression of NF-κB and NF-κB-Regulated Gene Expression by Apigenin through IκBα and IKK Pathway in TRAMP Mice

PubMed Central

Shukla, Sanjeev; Shankar, Eswar; Fu, Pingfu; MacLennan, Gregory T.; Gupta, Sanjay

2015-01-01

Aberrant Nuclear Factor-κappaB (NF-κB) activation due to rapid IκBα turnover and high basal IκBα kinase (IKK) activity has been frequently observed in prostate cancer. Apigenin, a naturally occurring plant flavone, exhibits anti-proliferative, anti-inflammatory and anti-carcinogenic activities by inhibiting NF-κB pathway, through a mechanism not fully understood. We found that apigenin feeding in microgram doses (bioavailable in humans) inhibited prostate tumorigenesis in TRAMP mice by interfering with NF-κB signaling. Apigenin feeding to TRAMP mice (20 and 50 μg/mouse/day, 6 days/week for 20 weeks) exhibited significant decrease in tumor volumes of the prostate and completely abolished metastasis, which correlated with inhibition of NF-κB activation and binding to the DNA. Apigenin intake blocked phosphorylation and degradation of IκBα by inhibiting IKK activation, which in turn led to suppression of NF-κB activation. The expression of NF-κB-regulated gene products involved in proliferation (cyclin D1, and COX-2), anti-apoptosis (Bcl-2 and Bcl-xL), and angiogenesis (vascular endothelial growth factor) were also downregulated after apigenin feeding. These events correlated with the induction of apoptosis in tumor cells, as evident by increased cleaved caspase-3 labeling index in the dorsolateral prostate. Our results provide convincing evidence that apigenin inhibits IKK activation and restores the expression of IκBα, preventing it’s phosphorylation in a fashion similar to that elicited by IKK and proteasomal inhibitors through suppression of NF-κB signaling pathway. PMID:26379052

S100A9 Interaction with TLR4 Promotes Tumor Growth

PubMed Central

Källberg, Eva; Vogl, Thomas; Liberg, David; Olsson, Anders; Björk, Per; Wikström, Pernilla; Bergh, Anders; Roth, Johannes; Ivars, Fredrik; Leanderson, Tomas

2012-01-01

By breeding TRAMP mice with S100A9 knock-out (S100A9−/−) animals and scoring the appearance of palpable tumors we observed a delayed tumor growth in animals devoid of S100A9 expression. CD11b+ S100A9 expressing cells were not observed in normal prostate tissue from control C57BL/6 mice but were readily detected in TRAMP prostate tumors. Also, S100A9 expression was observed in association with CD68+ macrophages in biopsies from human prostate tumors. Delayed growth of TRAMP tumors was also observed in mice lacking the S100A9 ligand TLR4. In the EL-4 lymphoma model tumor growth inhibition was observed in S100A9−/− and TLR4−/−, but not in RAGE−/− animals lacking an alternative S100A9 receptor. When expression of immune-regulating genes was analyzed using RT-PCR the only common change observed in mice lacking S100A9 and TLR4 was a down-regulation of TGFβ expression in splenic CD11b+ cells. Lastly, treatment of mice with a small molecule (ABR-215050) that inhibits S100A9 binding to TLR4 inhibited EL4 tumor growth. Thus, S100A9 and TLR4 appear to be involved in promoting tumor growth in two different tumor models and pharmacological inhibition of S100A9-TLR4 interactions is a novel and promising target for anti-tumor therapies. PMID:22470535

Whole-body irradiation increases the magnitude and persistence of adoptively transferred T cells associated with tumor regression in a mouse model of prostate cancer

PubMed Central

Ward-Kavanagh, Lindsay K.; Zhu, Junjia; Cooper, Timothy K.; Schell, Todd D.

2014-01-01

Adoptive immunotherapy has demonstrated efficacy in a subset of clinical and preclinical studies, but the T cells used for therapy often are rendered rapidly non-functional in tumor-bearing hosts. Recent evidence indicates that prostate cancer can be susceptible to immunotherapy, but most studies using autochthonous tumor models demonstrate only short-lived T-cell responses in the tolerogenic prostate microenvironment. Here, we assessed the efficacy of sublethal whole-body irradiation (WBI) to enhance the magnitude and duration of adoptively transferred CD8+ T cells in the transgenic adenocarcinoma of the mouse prostate (TRAMP) model. We demonstrate that WBI promoted high-level accumulation of granzyme B (GzB)-expressing donor T cells both in lymphoid organs and in the prostate of TRAMP mice. Donor T cells remained responsive to vaccination in irradiated recipients, but a single round of WBI-enhanced adoptive immunotherapy failed to impact significantly the existing disease. Addition of a second round of immunotherapy promoted regression of established disease in half of the treated mice, with no progressions observed. Regression was associated with long-term persistence of effector/memory phenotype CD8+ donor cells. Administration of the second round of adoptive immunotherapy led to reacquisition of GzB expression by persistent T cells from the first transfer. These results indicate that WBI conditioning amplifies tumor-specific T cells in the TRAMP prostate and lymphoid tissue, and suggest that the initial treatment alters the tolerogenic microenvironment to increase antitumor activity by a second wave of donor cells. PMID:24801834

TLR7 expression is decreased during tumour progression in transgenic adenocarcinoma of mouse prostate mice and its activation inhibits growth of prostate cancer cells.

PubMed

Han, Ju-Hee; Park, Shin-Young; Kim, Jin-Bum; Cho, Sung-Dae; Kim, Bumseok; Kim, Bo-Yeon; Kang, Min-Jung; Kim, Dong-Jae; Park, Jae-Hak; Park, Jong-Hwan

2013-10-01

Although various Toll-like receptors (TLRs) have been associated with immune response and tumorigenesis in the prostate cells, little is known about the role of TLR7. Accordingly, we examined the expression of TLR7 during tumour progression of TRMAP (transgenic mouse model for prostate cancer) mice and its role on cell growth. Toll-like receptor7 expression was examined by RT-polymerase chain reaction (PCR), Western blot, and immunohistochemistry. Cell growth was examined by MTT assay. Colony formation was investigated by crystal violet staining. Strong expression of TLR7 was detected in the normal prostate epithelia of Wild-type (WT) mice, but not in TLR7-deficient mice. In contrast, TLR7 expression was weak in transgenic adenocarcinoma of mouse prostate (TRAMP)-C2 cells, as compared with murine bone marrow-derived macrophages (BMDMs). Moreover, TLR7 mRNA was markedly expressed in RWPE-1 cells (non-cancerous prostate epithelial cells), but not in PC3 and DU145 (prostate cancer cells). Immunohistochemically, TLR7 expression gradually decreased in TRAMP mice depending on the pathologic grade of the prostate cells. TLR7 agonists increased both the gene and protein expression of TLR7 and promoted production of proinflammatory cytokines/chemokines and IFN-β gene expression in prostate cancer cell lines. Moreover, loxoribine inhibited the growth and colony formation of TRAMP-C2 cells dependent of TLR7. These findings suggest that TLR7 may participate in tumour suppression in the prostate cells. © 2013 John Wiley & Sons Ltd.

Phenotype-specific CpG island methylation events in a murine model of prostate cancer.

PubMed

Camoriano, Marta; Kinney, Shannon R Morey; Moser, Michael T; Foster, Barbara A; Mohler, James L; Trump, Donald L; Karpf, Adam R; Smiraglia, Dominic J

2008-06-01

Aberrant DNA methylation plays a significant role in nearly all human cancers and may contribute to disease progression to advanced phenotypes. Study of advanced prostate cancer phenotypes in the human disease is hampered by limited availability of tissues. We therefore took advantage of the Transgenic Adenocarcinoma of Mouse Prostate (TRAMP) model to study whether three different phenotypes of TRAMP tumors (PRIM, late-stage primary tumors; AIP, androgen-independent primary tumors; and MET, metastases) displayed specific patterns of CpG island hypermethylation using Restriction Landmark Genomic Scanning. Each tumor phenotype displayed numerous hypermethylation events, with the most homogeneous methylation pattern in AIP and the most heterogeneous pattern in MET. Several loci displayed a phenotype-specific methylation pattern; the most striking pattern being loci methylated at high frequency in PRIM and AIP but rarely in MET. Examination of the mRNA expression of three genes, BC058385, Goosecoid, and Neurexin 2, which exhibited nonpromoter methylation, revealed increased expression associated with downstream methylation. Only methylated samples showed mRNA expression, in which tumor phenotype was a key factor determining the level of expression. The CpG island in the human orthologue of BC058385 was methylated in human AIP but not in primary androgen-stimulated prostate cancer or benign prostate. The clinical data show a proof-of-principle that the TRAMP model can be used to identify targets of aberrant CpG island methylation relevant to human disease. In conclusion, phenotype-specific hypermethylation events were associated with the overexpression of different genes and may provide new markers of prostate tumorigenesis.

Curcumin Derivative Epigenetically Reactivates Nrf2 Antioxidative Stress Signaling in Mouse Prostate Cancer TRAMP C1 Cells.

PubMed

Li, Wenji; Su, Zheng-Yuan; Guo, Yue; Zhang, Chengyue; Wu, Renyi; Gao, Linbo; Zheng, Xi; Du, Zhi-Yun; Zhang, Kun; Kong, Ah-Ng

2018-02-19

The carcinogenesis of prostate cancer (PCa) in TRAMP model is highly correlated with hypermethylation in the promoter region of Nrf2 and the accompanying reduced transcription of Nrf2 and its regulated detoxifying genes. We aimed to investigate the effects of (3E,5E)-3,5-bis-(3,4,5-trimethoxybenzylidene)-tetrahydro-thiopyran-4-one (F10) and (3E,5E)-3,5-bis-(3,4,5-trimethoxy-benzylidene)-tetrahydropyran-4-one (E10), two synthetic curcumin derivatives, on restoring Nrf2 activity in TRAMP C1 cells. HepG2-C8 cells transfected with an antioxidant-response element (ARE)-luciferase vector were treated with F10, E10, curcumin, and sulforaphane (SFN) to compare their effects on Nrf2-ARE pathways. We performed real-time quantitative PCR and Western blotting to investigate the effects of F10 and E10 on Nrf2, correlated phase II detoxification genes. We also measured expression and activity of DNMTand HDAC enzymes. Enrichment of H3K27me3 on the promoter region of Nrf2 was explored with a chromatin immunoprecipitation (ChIP) assay. Methylation of the CpG region in Nrf2 promoter was doubly examined by bisulfite genomic sequencing (BGS) and methylation DNA immunoprecipitation (MeDIP). Compared with curcumin and SFN, F10 is more potent in activating Nrf2-ARE pathways. Both F10 and E10 enhanced level of Nrf2 and the correlated phase II detoxifying genes. BGS and MeDIP assays indicated that F10 but not E10 hypomethylated the Nrf2 promoter. F10 also downregulated the protein level of DNMT1, DNMT3a, DNMT3b, HDAC1, HDAC4, and HDAC7 and the activity of DNMTs and HDACs. F10 but not E10 effectively reduced the accumulation of H3k27me3 on the promoter of Nrf2. F10 and E10 can activate the Nrf2-ARE pathway and increase the level of Nrf2 and correlated phase II detoxification genes. The reactivation effect on Nrf2 by F10 in TRAMP C1 may come from demethylation, decrease of HDACs, and inhibition of H3k27me3 accumulation.

GPRC6A regulates prostate cancer progression

PubMed Central

Pi, Min; Quarles, L. Darryl

2011-01-01

BACKGROUND GPRC6A is a nutrient sensing GPCR that is activated in vitro by a variety of ligands, including amino acids, calcium, zinc, osteocalcin (OC) and testosterone. The association between nutritional factors and risk of prostate cancer, the finding of increased expression of OC in prostate cancer cells and the association between GPRC6A and risk of prostate cancer in Japanese men implicates a role of GPRC6A in prostate cancer. METHODS We examined if GPRC6A is expressed in human prostate cancer cell lines and used siRNA-mediated knockdown GPRC6A expression in prostate cancer cells to explore the function of GPRC6A in vitro. To assess the role GPRC6A in prostate cancer progression in vivo we intercrossed Gprc6a−/− mice onto the TRAMP mouse prostate cancer model. RESULTS GPRC6A transcripts were markedly increased in prostate cancer cell lines 22Rv1, PC-3 and LNCaP, compared to the normal prostate RWPE-1 cell line. In addition, a panel of GPRC6A ligands, including calcium, OC, and arginine, exhibited in prostate cancer cell lines a dose-dependent stimulation of ERK activity, cell proliferation, chemotaxis, and prostate specific antigen and Runx 2 gene expression. These responses were inhibited by siRNA-mediated knockdown of GPRC6A. Finally, transfer of Gprc6a deficiency onto a TRAMP mouse model of prostate cancer significantly retarded prostate cancer progression and improved survival of compound Gprc6a−/−/TRAMP mice. CONCLUSIONS GPRC6A is a novel molecular target for regulating prostate growth and cancer progression. Increments in GPRC6A may augment the ability of prostate cancer cells to proliferate in response to dietary and bone derived ligands. PMID:21681779

Trace element control in binary Ni-25Cr and ternary Ni-30Co-30Cr master alloy castings

DOE Office of Scientific and Technical Information (OSTI.GOV)

Detrois, Martin; Jablonski, Paul D.

Electro-slag remelting (ESR) is used for control of unwanted elements in commercial alloys. This study focuses on master alloys of Ni-25Cr and Ni-30Co-30Cr, processed through a combination of vacuum induction melting (VIM) and electro-slag remelting (ESR). Minor additions were made to control tramp element levels and modify the melting characteristics. Nitrogen and sulfur levels below 10 ppm and oxygen levels below 100 ppm were obtained in the final products. The role of the alloy additions in lowering the tramp element content, the resulting residual inclusions and the melting characteristics were determined computationally and confirmed experimentally. Additions of titanium were beneficialmore » to the control of oxygen levels during VIM and nitrogen levels during ESR. Aluminum additions helped to control oxygen levels during remelting, however, aluminum pickup occurred when excess titanium was present during ESR. The usefulness of these master alloys for use as experimental remelt stock will also be discussed.« less

2007 SB14 Source Reduction Plan/Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chang, L

2007-07-24

Aqueous solutions (mixed waste) generated from various LLNL operations, such as debris washing, sample preparation and analysis, and equipment maintenance and cleanout, were combined for storage in the B695 tank farm. Prior to combination the individual waste streams had different codes depending on the particular generating process and waste characteristics. The largest streams were CWC 132, 791, 134, 792. Several smaller waste streams were also included. This combined waste stream was treated at LLNL's waste treatment facility using a vacuum filtration and cool vapor evaporation process in preparation for discharge to sanitary sewer. Prior to discharge, the treated waste streammore » was sampled and the results were reviewed by LLNL's water monitoring specialists. The treated solution was discharged following confirmation that it met the discharge criteria. A major source, accounting for 50% for this waste stream, is metal machining, cutting and grinding operations in the engineering machine shops in B321/B131. An additional 7% was from similar operations in B131 and B132S. This waste stream primarily contains metal cuttings from machined parts, machining coolant and water, with small amounts of tramp oil from the machining and grinding equipment. Several waste reduction measures for the B321 machine shop have been taken, including the use of a small point-of-use filtering/tramp-oil coalescing/UV-sterilization coolant recycling unit, and improved management techniques (testing and replenishing) for coolants. The recycling unit had some operational problems during 2006. The machine shop is planning to have it repaired in the near future. A major source, accounting for 50% for this waste stream, is metal machining, cutting and grinding operations in the engineering machine shops in B321/B131. An additional 7% was from similar operations in B131 and B132S. This waste stream primarily contains metal cuttings from machined parts, machining coolant and water, with small amounts of tramp oil from the machining and grinding equipment. Several waste reduction measures for the B321 machine shop have been taken, including the use of a small point-of-use filtering/tramp-oil coalescing/UV-sterilization coolant recycling unit, and improved management techniques (testing and replenishing) for coolants. The recycling unit had some operational problems during 2006. The machine shop is planning to have it repaired in the near future. Quarterly waste generation data prepared by the Environmental Protection Department's P2 Team are regularly provided to engineering shops as well as other facilities so that generators can track the effectiveness of their waste minimization efforts.« less

Parthenolide Selectively Sensitizes Prostate Tumor Tissue to Radiotherapy while Protecting Healthy Tissues In Vivo.

PubMed

Morel, Katherine L; Ormsby, Rebecca J; Bezak, Eva; Sweeney, Christopher J; Sykes, Pamela J

2017-05-01

Radiotherapy is widely used in cancer treatment, however the benefits can be limited by radiation-induced damage to neighboring normal tissues. Parthenolide (PTL) exhibits anti-inflammatory and anti-tumor properties and selectively induces radiosensitivity in prostate cancer cell lines, while protecting primary prostate epithelial cell lines from radiation-induced damage. Low doses of radiation have also been shown to protect from subsequent high-dose-radiation-induced apoptosis as well as DNA damage. These properties of PTL and low-dose radiation could be used to improve radiotherapy by killing more tumor cells and less normal cells. Sixteen-week-old male Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP) and C57BL/6J mice were treated with PTL (40 mg/kg), dimethylaminoparthenolide (DMAPT, a PTL analogue with increased bioavailability) (100 mg/kg), or vehicle control three times over one week prior to combinations of low (10 mGy) and high (6 Gy) doses of whole-body X-irradiation. Tissues were analyzed for apoptosis at a range of time points up to 72 h postirradiation. Both PTL and DMAPT protected normal tissues, but not prostate tumor tissues, from a significant proportion of high-dose-radiation-induced apoptosis. DMAPT provided superior protection compared to PTL in normal dorsolateral prostate (71.7% reduction, P = 0.026), spleen (48.2% reduction, P = 0.0001) and colorectal tissue (38.0% reduction, P = 0.0002), and doubled radiation-induced apoptosis in TRAMP prostate tumor tissue (101.3% increase, P = 0.039). Both drugs induced the greatest radiosensitivity in TRAMP prostate tissue in areas with higher grade prostatic intraepithelial neoplasia (PIN) lesions. A 10 mGy dose delivered 3 h prior to a 6 Gy dose induced a radioadaptive apoptosis response in normal C57Bl/6J prostate (28.4% reduction, P = 0.045) and normal TRAMP spleen (13.6% reduction, P = 0.047), however the low-dose-adaptive radioprotection did not significantly add to the PTL/DMAPT-induced protection in normal tissues, nor did it affect tumor kill. These results support the use of the more bioavailable DMAPT and low-dose radiation, alone or in combination as useful radioprotectors of normal tissues to alleviate radiotherapy-induced side-effects in patients. The enhanced radiosensitisation in prostate tissues displaying high-grade PIN suggests that DMAPT also holds promise for targeted therapy of advanced prostate cancer, which may go on to become metastatic. The redox mechanisms involved in the differential radioprotection observed here suggest that increased radiotherapy efficacy by DMAPT is more broadly applicable to a range of cancer types.

Combinational Targeting of Prostate Carcinoma Cells and Tumors Associated Pericytes with Antibody Based Immunotherapy and Metronomic Chemotherapy

DTIC Science & Technology

2010-02-01

Carcinoma Cells and Tumors Associated Pericytes with Antibody Based Immunotherapy and Metronomic Chemotherapy PRINCIPAL INVESTIGATOR......purity and activity. The colony of TRAMP mice has been expanded to test the efficacy of mAb 225.28 plus cyclophosphamide metronomic therapy in the

A Tramp Abroad Chronicles Motivation, Self-Efficacy, and Attempts to Theorize Culture and Creativity

ERIC Educational Resources Information Center

Crutcher, Paul A.; Dodge, Autumn M.

2014-01-01

The paper works organically from unique experiences the authors have teaching abroad with other trained teachers and scholars and in diverse contexts. It considers the culture and creativity literature, fundamental and relevant mechanisms in Teaching English to Speakers of Other Languages and educational psychology, and suggests a relational…

Effect of Dietary Intervention on Prostate Tumor Development in TRAMP Mice

DTIC Science & Technology

2006-05-01

Proc.Natl.Acad.Sci.USA, 92, 3439-3443. 5. Gingrich,J.R., Barrios ,R.J., Morton,R.A., Boyce,B.F., DeMayo,F.J., Finegold,M.J., Angelopoulou,R., Rosen,J.M...the research effort Margot P. Cleary, Principal Investigator Michael Grossmann, Research Associate Patricia Grambsch, Statistician Olga Rogozin

How High Is the Tramping Track? Mathematising and Applying in a Calculus Model-Eliciting Activity

ERIC Educational Resources Information Center

Yoon, Caroline; Dreyfus, Tommy; Thomas, Michael O. J.

2010-01-01

Two complementary processes involved in mathematical modelling are mathematising a realistic situation and applying a mathematical technique to a given realistic situation. We present and analyse work from two undergraduate students and two secondary school teachers who engaged in both processes during a mathematical modelling task that required…

Amylose Phase Composition As Analyzed By FTIR In A Temperature Ramp: Influence Of Short Range Order On The Thermodynamic Properties

NASA Astrophysics Data System (ADS)

Bernazzani, Paul; Delmas, Genevieve

1998-03-01

Amylose, a major component of starch, is one of the most important biopolymers, being mainly associated with the pharmacological and food industries. Although widely studied, a complete control and understanding of the physical properties of amylose is still lacking. It is well known that structure and phase transition are important aspects of the functionality of biopolymers since they influence physical attributes such as appearance, digestibility, water holding capacity, etc. In the past, we have studied polyethylene phase composition by DSC in a very slow temperature (T) ramp (1K/h) and have demonstrated the presence and importance of short-range order on the polymer and its characteristics. In this study, we evaluated the phase composition of potato amylose and associated the thermodynamic properties with the presence of short-range order. Two methods were correlated, DSC (in a 1K/h T-ramp) and FTIR as a function of temperature, also in a 1K/h T-ramp. The effects of the various phases on thermodynamic properties such as gelation and enzyme or chemical resistance are discussed.

Adoptive cell therapy of prostate cancer using female mice-derived T cells that react with prostate antigens

PubMed Central

Yi, Huanfa; Yu, Xiaofei; Guo, Chunqing; Manjili, Masoud H.; Repasky, Elizabeth A.; Wang, Xiang-Yang

2011-01-01

In this study, we report a novel treatment strategy that could potentially be used to improve efficacy of adoptive cell therapy for patients with prostate cancer. We show that female C57BL/6 mice are able to effectively reject two syngeneic prostate tumors (TRAMP-C2 and RM1) in a T cell-dependent manner. The protective antitumor immunity appears to primarily involve T cell responses reactive against general prostate tumor/tissue antigens, rather than simply to male-specific H-Y antigen. For the first time we show that adoptive transfer of lymphocytes from TRAMP-C2-primed or naive female mice effectively control prostate tumor growth in male mice, when combined with host pre-conditioning (i.e., non-myeloablative lymphodepletion) and IL-2 administration. No pathological autoimmune response was observed in the treated tumor-bearing male mice. Our studies provide new insights regarding the immune-mediated recognition of male-specific tissue, such as the prostate, and may offer new immunotherapy treatment strategies for advanced prostate cancer. PMID:21088965

Apigenin inhibits prostate cancer progression in TRAMP mice via targeting PI3K/Akt/FoxO pathway

PubMed Central

Gupta, Sanjay

2014-01-01

Forkhead box O (FoxO) transcription factors play an important role as tumor suppressor in several human malignancies. Disruption of FoxO activity due to loss of phosphatase and tensin homolog and activation of phosphatidylinositol-3 kinase (PI3K)/Akt are frequently observed in prostate cancer. Apigenin, a naturally occurring plant flavone, exhibits antiproliferative and anticarcinogenic activities through mechanisms, which are not fully defined. In the present study, we show that apigenin suppressed prostate tumorigenesis in transgenic adenocarcinoma of the mouse prostate (TRAMP) mice through the PI3K/Akt/FoxO-signaling pathway. Apigenin-treated TRAMP mice (20 and 50 μg/mouse/day, 6 days/week for 20 weeks) exhibited significant decrease in tumor volumes of the prostate as well as completely abolished distant organ metastasis. Apigenin treatment resulted in significant decrease in the weight of genitourinary apparatus (P < 0.0001), dorsolateral (P < 0.0001) and ventral prostate (P < 0.028), compared with the control group. Apigenin-treated mice showed reduced phosphorylation of Akt (Ser473) and FoxO3a (Ser253), which correlated with its increased nuclear retention and decreased binding of FoxO3a with 14-3-3. These events lead to reduced proliferation as assessed by Ki-67 and cyclin D1, along with upregulation of FoxO-responsive proteins BIM and p27/Kip1. Complementing in vivo results, similar observations were noted in human prostate cancer LNCaP and PC-3 cells after apigenin treatment. Furthermore, binding of FoxO3a with p27/Kip1 was markedly increased after 10 and 20 μM apigenin treatment resulting in G0/G1-phase cell cycle arrest, which was consistent with the effects elicited by PI3K/Akt inhibitor, LY294002. These results provide convincing evidence that apigenin effectively suppressed prostate cancer progression, at least in part, by targeting the PI3K/Akt/FoxO-signaling pathway. PMID:24067903

ANALYSIS OF TMEFF2 ALLOGRAFTS AND TRANSGENIC MOUSE MODELS REVEALS ROLES IN PROSTATE REGENERATION AND CANCER

PubMed Central

Corbin, JM.; Overcash, RF.; Wren, JD.; Coburn, A.; Tipton, GJ.; Ezzell, JA.; McNaughton, KK.; Fung, KM; Kosanke, SD.; Ruiz-Echevarria, MJ

2015-01-01

BACKGROUND Previous results from our lab indicate a tumor suppressor role for the transmembrane protein with epidermal growth factor and two follistatin motifs 2 (TMEFF2) in prostate cancer (PCa). Here, we further characterize this role and uncover new functions for TMEFF2 in cancer and adult prostate regeneration. METHODS The role of TMEFF2 was examined in PCa cells using Matrigel™ cultures and allograft models of PCa cells. In addition, we developed a transgenic mouse model that expresses TMEFF2 from a prostate specific promoter. Anatomical, histological and metabolic characterizations of the transgenic mouse prostate were conducted. The effect of TMEFF2 in prostate regeneration was studied by analyzing branching morphogenesis in the TMEFF2-expressing mouse lobes and alterations in branching morphogenesis were correlated with the metabolomic profiles of the mouse lobes. The role of TMEFF2 in prostate tumorigenesis in whole animals was investigated by crossing the TMEFF2 transgenic mice with the TRAMP mouse model of PCa and analyzing the histopathological changes in the progeny. RESULTS Ectopic expression of TMEFF2 impairs growth of PCa cells in Matrigel or allograft models. Surprisingly, while TMEFF2 expression in the TRAMP mouse did not have a significant effect on the glandular prostate epithelial lesions, the double TRAMP/TMEFF2 transgenic mice displayed an increased incidence of neuroendocrine type tumors. In addition, TMEFF2 promoted increased branching specifically in the dorsal lobe of the prostate suggesting a potential role in developmental processes. These results correlated with data indicating an alteration in the metabolic profile of the dorsal lobe of the transgenic TMEFF2 mice. CONCLUSIONS Collectively, our results confirm the tumor suppressor role of TMEFF2 and suggest that ectopic expression of TMEFF2 in mouse prostate leads to additional lobe-specific effects in prostate regeneration and tumorigenesis. This points to a complex and multifunctional role for TMEFF2 during PCa progression. PMID:26417683

Analysis of TMEFF2 allografts and transgenic mouse models reveals roles in prostate regeneration and cancer.

PubMed

Corbin, Joshua M; Overcash, Ryan F; Wren, Jonathan D; Coburn, Anita; Tipton, Greg J; Ezzell, Jennifer A; McNaughton, Kirk K; Fung, Kar-Ming; Kosanke, Stanley D; Ruiz-Echevarria, Maria J

2016-01-01

Previous results from our lab indicate a tumor suppressor role for the transmembrane protein with epidermal growth factor and two follistatin motifs 2 (TMEFF2) in prostate cancer (PCa). Here, we further characterize this role and uncover new functions for TMEFF2 in cancer and adult prostate regeneration. The role of TMEFF2 was examined in PCa cells using Matrigel(TM) cultures and allograft models of PCa cells. In addition, we developed a transgenic mouse model that expresses TMEFF2 from a prostate specific promoter. Anatomical, histological, and metabolic characterizations of the transgenic mouse prostate were conducted. The effect of TMEFF2 in prostate regeneration was studied by analyzing branching morphogenesis in the TMEFF2-expressing mouse lobes and alterations in branching morphogenesis were correlated with the metabolomic profiles of the mouse lobes. The role of TMEFF2 in prostate tumorigenesis in whole animals was investigated by crossing the TMEFF2 transgenic mice with the TRAMP mouse model of PCa and analyzing the histopathological changes in the progeny. Ectopic expression of TMEFF2 impairs growth of PCa cells in Matrigel or allograft models. Surprisingly, while TMEFF2 expression in the TRAMP mouse did not have a significant effect on the glandular prostate epithelial lesions, the double TRAMP/TMEFF2 transgenic mice displayed an increased incidence of neuroendocrine type tumors. In addition, TMEFF2 promoted increased branching specifically in the dorsal lobe of the prostate suggesting a potential role in developmental processes. These results correlated with data indicating an alteration in the metabolic profile of the dorsal lobe of the transgenic TMEFF2 mice. Collectively, our results confirm the tumor suppressor role of TMEFF2 and suggest that ectopic expression of TMEFF2 in mouse prostate leads to additional lobe-specific effects in prostate regeneration and tumorigenesis. This points to a complex and multifunctional role for TMEFF2 during PCa progression. © 2015 Wiley Periodicals, Inc.

Walnuts lower TRAMP prostate tumor growth by altering IGF-1, energy and cholesterol metabolism and is not due to their fatty acids

USDA-ARS?s Scientific Manuscript database

Dietary changes could potentially reduce prostate cancer morbidity and mortality. Prostate tumor size, gene expression, metabolite and plasma responses to a 100 g of fat/kg diet (whole walnuts, walnut oil and other oils; balanced for macronutrients, tocopherols (a-and ' ) for 18 weeks were assessed ...

Polyphenols and Prostate Cancer Chemoprevention

DTIC Science & Technology

2005-03-01

ABSTRACT (Maximum 200 Words) The goal of this research is to Investigate the potential of (-)- epigallocatechin -3- gallate ( EGCG ), genistein and resveratrol...prostate chemoprevention are the soy isoflavone, genistein, and the tea catechin, (-)- epigallocatechin -3- gallate ( EGCG ). Another polyphenol that has...adenocarcinoma (TRAMP)). The specific aims are 1) to investigate the potential of genistein, EGCG and resveratrol, alone and in combination, to

Examination of the Role of DNA Methylation Changes in Prostate Cancer Using the Transgenic Adenocarcinoma of Mouse Prostate (TRAMP) Model

DTIC Science & Technology

2008-03-01

overcomes bias in bisulfite PCR methylation analysis. Biotechniques, 42: 48, 50, 52 passim, 2007. 46. Warnecke, P. M., Stirzaker, C., Melki , J. R...overcomes bias in bisulfite PCR methylation analysis. Biotechniques 2007;42:48, 50, 2 passim. 36. Warnecke PM, Stirzaker C, Melki JR, Millar DS, Paul CL

Understanding the Virtualization of the Backpacker Culture and the Emergence of the Flashpacker: A Mixed-Method

ERIC Educational Resources Information Center

Paris, Cody Morris

2010-01-01

Backpackers are pioneers of mobility, who provide a unique domain for critical tourism research. The lineage of backpacker ideals, including pursuit of authentic experiences, independence, escape and social interaction, can be traced back to the "tramps" of the 1880s and the "drifters" of the 1970s. The recent emergence of the…

Combinatorial Targeting of Prostate Carcinoma Cells and Tumor Associated Pericytes with Antibody-Based Immunotherapy and Metronomic Chemotherapy

DTIC Science & Technology

2011-03-01

Carcinoma Cells and Tumor Associated Pericytes with Antibody-Based Immunotherapy and Metronomic Chemotherapy. PRINCIPAL INVESTIGATOR: Soldano...Combinatorial Targeting of Prostate Carcinoma Cells and Tumor Associated Pericytes with Antibody-Based Immunotherapy and Metronomic Chemotherapy. 5b. GRANT...SUPPLEMENTARY NOTES 14. ABSTRACT Seventy seven 10 week old TRAMP mice were enrolled in the study. Administration of metronomic chemotherapy with

Sanguinarine: A Novel Agent Against Prostate Cancer

DTIC Science & Technology

2008-01-01

the possibility of treatment- toxicity , the effect of treatments on food/water consumption and body weight was monitored twice weekly throughout the...data not shown). Further, the treatments were not found to have any evident toxic effects (body weight, food/fluid consumption) on the TRAMP mice...c]phenanthridine alkaloids sanguinarine and chelerythrine: biological activities and dental care applications. Acta Univ Palacki Olomuc Fac Med 139:7

"Who Are These Men?" A Study of the Tramps of Downtown Stockton (And the Agencies That Serve Them). Research Monograph No. 10.

ERIC Educational Resources Information Center

Adelman, Lester C.; Durant, Bill E.

A series of research and service projects designed to better the condition of single male farmworkers in Stockton and Sacramento were sponsored by the Department of Applied Behavioral Sciences at the University of California, Davis. The Stockton Singlemen's Project, begun in January 1971, had two basic elements: (1) research and (2) community…

Effect of Dietary Intervention on Prostate Tumor Development in Tramp Mice

DTIC Science & Technology

2005-05-01

successful. Dr. Olga Rogozina who has both an MD. and Ph.D. join our research group May of 2004. She is working 50% effort on this project and will be...3443. 5. Gingrich,J.R., Barrios ,R.J., Morton,R.A., Boyce,B.F., DeMayo,F.J., Finegold,M.J., Angelopoulou,R., Rosen,J.M., and Greenberg,N.M. (1996

Preventive and Therapeutic Efficacy of Finasteride and Dutasteride in TRAMP Mice

PubMed Central

Opoku-Acheampong, Alexander B.; Unis, Dave; Henningson, Jamie N.; Beck, Amanda P.; Lindshield, Brian L.

2013-01-01

Background The prostate cancer prevention trial (PCPT) and Reduction by dutasteride of Prostate Cancer Events (REDUCE) trial found that 5α-reductase (5αR) inhibitors finasteride and dutasteride respectively, decreased prostate cancer prevalence but also increased the incidence of high-grade tumors. 5αR2 is the main isoenzyme in normal prostate tissue; however, most prostate tumors have high 5αR1 and low 5αR2 expression. Because finasteride inhibits only 5αR2, we hypothesized that it would not be as efficacious in preventing prostate cancer development and/or progression in C57BL/6 TRAMP x FVB mice as dutasteride, which inhibits both 5αR1 and 5αR2. Method/Principal Findings Six-week-old C57BL/6 TRAMP x FVB male mice were randomized to AIN93G control or pre- and post- finasteride and dutasteride diet (83.3 mg drug/kg diet) groups (n =30–33) that began at 6 and 12 weeks of age, respectively, and were terminated at 20 weeks of age. The pre- and post- finasteride and dutasteride groups were designed to test the preventive and therapeutic efficacy of the drugs, respectively. Final body weights, genitourinary tract weights, and genitourinary tract weights as percentage of body weights were significantly decreased in the Pre- and Post-dutasteride groups compared with the control. The Post-dutasteride group showed the greatest inhibition of prostatic intraepithelial neoplasia progression and prostate cancer development. Surprisingly, the Post-dutasteride group showed improved outcomes compared with the Pre-dutasteride group, which had increased incidence of high-grade carcinoma as the most common and most severe lesions in a majority of prostate lobes. Consistent with our hypothesis, we found little benefit from the finasteride diets, and they increased the incidence of high-grade carcinoma. Conclusion Our findings have commonalities with previously reported PCPT, REDUCE, and the Reduction by dutasteride of Clinical Progression Events in Expectant Management (REDEEM) trial results. Our results may support the therapeutic use of dutasteride, but not finasteride, for therapeutic or preventive use. PMID:24204943

REM sleep behavior disorder and other sleep disturbances in Disney animated films.

PubMed

Iranzo, Alex; Schenck, Carlos H; Fonte, Jorge

2007-08-01

During a viewing of Disney's animated film Cinderella (1950), one author (AI) noticed a dog having nightmares with dream-enactment that strongly resembled RBD. This prompted a study in which all Disney classic full-length animated films and shorts were analyzed for other examples of RBD. Three additional dogs were found with presumed RBD in the classic films Lady and the Tramp (1955) and The Fox and the Hound (1981), and in the short Pluto's Judgment Day (1935). These dogs were elderly males who would pant, whine, snuffle, howl, laugh, paddle, kick, and propel themselves while dreaming that they were chasing someone or running away. In Lady and the Tramp the dog was also losing both his sense of smell and his memory, two associated features of human RBD. These four films were released before RBD was first formally described in humans and dogs. In addition, systematic viewing of the Disney films identified a broad range of sleep disorders, including nightmares, sleepwalking, sleep related seizures, disruptive snoring, excessive daytime sleepiness, insomnia and circadian rhythm sleep disorder. These sleep disorders were inserted as comic elements. The inclusion of a broad range of accurately depicted sleep disorders in these films indicates that the Disney screenwriters were astute observers of sleep and its disorders.

CDK5-A Novel Role in Prostate Cancer Immunotherapy

DTIC Science & Technology

2017-10-01

of the involvement of a T cell antitumor response in impaired growth of prostate cancer in immunocompetent murine models of prostate cancer, and...of immune system activation by Cdk5 deletion in prostate cancer. We will confirm the involvement of a T cell antitumor response in impaired growth of...project? Major Task 1: Involvement of T cell anticancer immune response in impaired growth of TRAMP Cdk5-/- model. Months 1-10. Completed, month 10

Comparative Analysis of Vitamin A (Retinol) Regulated Genes in African-American and Caucasian Prostate Cancer Patients

DTIC Science & Technology

2007-03-01

chemoprevention strategies and to the development of novel therapies for this disease. 14. SUBJECT TERMS 15. NUMBER OF PAGES 13Retinoids, Vitamin A...the TRAMP model will ultimately lead to improved chemoprevention strategies and to the development of novel therapies for prostate cancer...Selective retinoids and rexinoids in cancer therapy and chemoprevention. Drug Discov Today, 7: 1165-1174, 2002. 5. Wei, L. N. Retinoid receptors and

CDK5 A Novel Role in Prostate Cancer Immunotherapy

DTIC Science & Technology

2016-10-01

Distribution Unlimited The views, opinions and/or findings contained in this report are those of the author(s) and should not be construed as an official...We are now discussing our next steps in pursuing this finding; these may include treatment of TRAMP Cdk5 knockout mice to inhibit the putative...Merck and Bayer have terminated clinical development of these compounds. The compounds are still well suited as “tool compounds,” for the

A high-fat diet containing whole walnuts (Juglans regia) reduces tumour size and growth along with plasma insulin-like growth factor 1 in the transgenic adenocarcinoma of the mouse prostate model

USDA-ARS?s Scientific Manuscript database

Dietary fat is linked to prostate cancer (PCa), the most commonly diagnosed male cancer, but the nature and strength of the relationships between total fat, n-6 and n-3 fatty acids and PCa remain incompletely understood. Transgenic adenocarcinoma of the mouse prostate (TRAMP) mice (N=10-12 per grou...

Modifier locus mapping of a transgenic F2 mouse population identifies CCDC115 as a novel aggressive prostate cancer modifier gene in humans.

PubMed

Winter, Jean M; Curry, Natasha L; Gildea, Derek M; Williams, Kendra A; Lee, Minnkyong; Hu, Ying; Crawford, Nigel P S

2018-06-11

It is well known that development of prostate cancer (PC) can be attributed to somatic mutations of the genome, acquired within proto-oncogenes or tumor-suppressor genes. What is less well understood is how germline variation contributes to disease aggressiveness in PC patients. To map germline modifiers of aggressive neuroendocrine PC, we generated a genetically diverse F2 intercross population using the transgenic TRAMP mouse model and the wild-derived WSB/EiJ (WSB) strain. The relevance of germline modifiers of aggressive PC identified in these mice was extensively correlated in human PC datasets and functionally validated in cell lines. Aggressive PC traits were quantified in a population of 30 week old (TRAMP x WSB) F2 mice (n = 307). Correlation of germline genotype with aggressive disease phenotype revealed seven modifier loci that were significantly associated with aggressive disease. RNA-seq were analyzed using cis-eQTL and trait correlation analyses to identify candidate genes within each of these loci. Analysis of 92 (TRAMP x WSB) F2 prostates revealed 25 candidate genes that harbored both a significant cis-eQTL and mRNA expression correlations with an aggressive PC trait. We further delineated these candidate genes based on their clinical relevance, by interrogating human PC GWAS and PC tumor gene expression datasets. We identified four genes (CCDC115, DNAJC10, RNF149, and STYXL1), which encompassed all of the following characteristics: 1) one or more germline variants associated with aggressive PC traits; 2) differential mRNA levels associated with aggressive PC traits; and 3) differential mRNA expression between normal and tumor tissue. Functional validation studies of these four genes using the human LNCaP prostate adenocarcinoma cell line revealed ectopic overexpression of CCDC115 can significantly impede cell growth in vitro and tumor growth in vivo. Furthermore, CCDC115 human prostate tumor expression was associated with better survival outcomes. We have demonstrated how modifier locus mapping in mouse models of PC, coupled with in silico analyses of human PC datasets, can reveal novel germline modifier genes of aggressive PC. We have also characterized CCDC115 as being associated with less aggressive PC in humans, placing it as a potential prognostic marker of aggressive PC.

Thermodynamic Considerations of Contamination by Alloying Elements of Remelted End-of-Life Nickel- and Cobalt-Based Superalloys

NASA Astrophysics Data System (ADS)

Lu, Xin; Matsubae, Kazuyo; Nakajima, Kenichi; Nakamura, Shinichiro; Nagasaka, Tetsuya

2016-06-01

Cobalt and nickel are high-value commodity metals and are mostly used in the form of highly alloyed materials. The alloying elements used may cause contamination problems during recycling. To ensure maximum resource efficiency, an understanding of the removability of these alloying elements and the controllability of some of the primary alloying elements is essential with respect to the recycling of end-of-life (EoL) nickel- and cobalt-based superalloys by remelting. In this study, the distribution behaviors of approximately 30 elements that are usually present in EoL nickel- and cobalt-based superalloys in the solvent metal (nickel, cobalt, or nickel-cobalt alloy), oxide slag, and gas phases during the remelting were quantitatively evaluated using a thermodynamic approach. The results showed that most of the alloying elements can be removed either in the slag phase or into the gas phase. However, the removal of copper, tin, arsenic, and antimony by remelting is difficult, and they remain as tramp elements during the recycling. On the other hand, the distribution tendencies of iron, molybdenum, and tungsten can be controlled by changing the remelting conditions. To increase the resource efficiency of recycling, preventing contamination by the tramp elements and identifying the alloying compositions of EoL superalloys are significantly essential, which will require the development of efficient prior alloy-sorting systems and advanced separation technologies.

LIGHT: A Novel Immunotherapy for Primary and Metastatic Prostate Cancer

DTIC Science & Technology

2013-09-01

and TRAMP-C2 LIGHT expressing cells to examine the frequency of Tregs subsequent to LIGHT interaction . These results reflect on the ability of LIGHT...cells. These data suggest that LIGHT interaction directly affects the induction of Tregs from a naïve CD4+ T cell population but also that this is not... interaction directly affects the induction of Tregs from a naïve CD4+ T cell population.  mPSCA TriVax induces infiltration of NK and MDSCs, whereas

Role of Obesity in Prostate Cancer Development

DTIC Science & Technology

2011-04-01

Western blot analysis . Representative staining from the immunohistochemistry is shown in Figure 6. Expression of AdipoR1 was found in all prostate tumor...with goat serum instead of primary antibody was negative (Fig 6D). Western blot analysis of frozen tissue from the same mice was also performed and...TRAMP) model. Cancer Res., 57, 3325-3330. 41. Williams,T.M., Hassan,G.S., Li,J., Cohen,A.W., Medina,F., Frank,P.G., Pestell ,R.G., Di Vizio,D., and

Environmental Assessment and Environmental Baseline Survey for the Lease Acquisition of T-Ramp Property from Allegheny County Airport Authority

DTIC Science & Technology

2012-08-09

identified as wetlands or 50- or 100-year flood plains, neither wetlands nor flood plains will be adversely impacted by either the proposed action or the...In addition, the proposed action and no action alternative are not located within or would impact wetland areas. Therefore, a Finding ofNo...Practicable Alternative (FONPA) to avoid wetland impacts is not required for the proposed action. C C. PETERS, Col, USAFR DATE Commander, 911 1h Airlift

A novel sulindac derivative lacking COX-inhibitory activities suppresses carcinogenesis in the transgenic adenocarcinoma of mouse prostate model

PubMed Central

Zhang, Yong; Zhang, Jinhui; Wang, Lei; Quealy, Emily; Gary, Bernard D.; Reynolds, Robert C.; Piazza, Gary A.; Lü, Junxuan

2016-01-01

Nonsteroidal anti-inflammatory drugs (NSAIDs) including sulindac are well-documented to be highly effective for cancer chemoprevention. However, their cyclooxygenase (COX) inhibitory activities cause severe gastrointestinal and cardiovascular toxicities, limiting their chronic use. Recent studies suggest that COX-independent mechanisms may be responsible for the chemopreventive benefits of the NSAIDs, and support the potential for development of a novel generation of sulindac derivatives lacking COX inhibition for cancer chemoprevention. A prototypic sulindac derivative with a N,N-dimethylammonium substitution, referred to as sulindac sulfide amide (SSA) was recently identified to be devoid of COX inhibitory activity yet displays much more potent tumor cell growth inhibitory activity in vitro compared to sulindac sulfide. In this study, we investigated the androgen receptor (AR) signaling pathway as a potential target for its COX-independent antineoplastic mechanism and evaluated its chemopreventive efficacy against prostate carcinogenesis using the TRAMP mouse model. The results showed that SSA significantly suppressed the growth of human and mouse prostate cancer cells expressing AR in strong association with G1 arrest, and decreased AR level and AR-dependent transactivation. Dietary SSA consumption from 6 to 24 weeks of age dramatically attenuated prostatic growth and suppressed AR-dependent glandular epithelial lesion progression via repressing cell proliferation in the TRAMP mice, whereas it did not significantly impact neuroendocrine carcinoma growth. Overall, the results suggest that SSA may be a chemopreventive candidate against prostate glandular epithelial carcinogenesis. PMID:20587701

Enhancement of broccoli indole glucosinolates by methyl jasmonate treatment and effects on prostate carcinogenesis.

PubMed

Liu, Ann G; Juvik, John A; Jeffery, Elizabeth H; Berman-Booty, Lisa D; Clinton, Steven K; Erdman, John W

2014-11-01

Broccoli is rich in bioactive components, such as sulforaphane and indole-3-carbinol, which may impact cancer risk. The glucosinolate profile of broccoli can be manipulated through treatment with the plant stress hormone methyl jasmonate (MeJA). Our objective was to produce broccoli with enhanced levels of indole glucosinolates and determine its impact on prostate carcinogenesis. Brassica oleracea var. Green Magic was treated with a 250 μM MeJA solution 4 days prior to harvest. MeJA-treated broccoli had significantly increased levels of glucobrassicin, neoglucobrassicin, and gluconasturtiin (P < .05). Male transgenic adenocarcinoma of mouse prostate (TRAMP) mice (n = 99) were randomized into three diet groups at 5-7 weeks of age: AIN-93G control, 10% standard broccoli powder, or 10% MeJA broccoli powder. Diets were fed throughout the study until termination at 20 weeks of age. Hepatic CYP1A was induced with MeJA broccoli powder feeding, indicating biological activity of the indole glucosinolates. Following ∼ 15 weeks on diets, neither of the broccoli treatments significantly altered genitourinary tract weight, pathologic score, or metastasis incidence, indicating that broccoli powder at 10% of the diet was ineffective at reducing prostate carcinogenesis in the TRAMP model. Whereas broccoli powder feeding had no effect in this model of prostate cancer, our work demonstrates the feasibility of employing plant stress hormones exogenously to stimulate changes in phytochemical profiles, an approach that may be useful for optimizing bioactive component patterns in foods for chronic-disease-prevention studies.

Modulation of AKR1C2 by curcumin decreases testosterone production in prostate cancer.

PubMed

Ide, Hisamitsu; Lu, Yan; Noguchi, Takahiro; Muto, Satoru; Okada, Hiroshi; Kawato, Suguru; Horie, Shigeo

2018-04-01

Intratumoral androgen biosynthesis has been recognized as an essential factor of castration-resistant prostate cancer. The present study investigated the effects of curcumin on the inhibition of intracrine androgen synthesis in prostate cancer. Human prostate cancer cell lines, LNCaP and 22Rv1 cells were incubated with or without curcumin after which cell proliferation was measured at 0, 24, 48 and 72 hours, respectively. Prostate tissues from the transgenic adenocarcinoma of the mouse prostate (TRAMP) model were obtained after 1-month oral administration of 200 mg/kg/d curcumin. Testosterone and dihydrotestosterone concentrations in LNCaP prostate cancer cells were determined through LC-MS/MS assay. Curcumin inhibited cell proliferation and induced apoptosis of prostate cancer cells in a dose-dependent manner. Curcumin decreased the expression of steroidogenic acute regulatory proteins, CYP11A1 and HSD3B2 in prostate cancer cell lines, supporting the decrease of testosterone production. After 1-month oral administration of curcumin, Aldo-Keto reductase 1C2 (AKR1C2) expression was elevated. Simultaneously, decreased testosterone levels in the prostate tissues were observed in the TRAMP mice. Meanwhile, curcumin treatments considerably increased the expression of AKR1C2 in prostate cancer cell lines, supporting the decrease of dihydrotestosterone. Taken together, these results suggest that curcumin's natural bioactive compounds could have potent anticancer properties due to suppression of androgen production, and this could have therapeutic effects on prostate cancer. © 2018 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

On the morphology of the digestive system of two Monomorium ant species.

PubMed

Solis, Daniel Russ; Rossi, Mônica Lanzoni; Fox, Eduardo Gonçalves Paterson; Nogueira, Neusa de Lima; Tanaka, Francisco André Ossamu; Bueno, Odair Correa

2013-01-01

The digestive system of adults and mature larvae of two ant species of Monomorium Mayr (Hymoneptera: Formicidae) were described with the aid of light and scanning electron microscopy, as there is a lack of studies in this area. These two ant species are recurrently found in urban habitats and are known as 'tramp species,' as they cause problems in households, businesses, and hospitals. The most interesting finds of the present study include the existence of spinules in the crop of adults, and the number of Malpighian tubules and rectal pads was constant among different castes, ages, and species.

The Role of I-kappa-B Kinases in Prostate Carcinogenesis and the Effect of Their Inhibition on Survival of Prostate Tumors

DTIC Science & Technology

2007-01-01

incorporation, which identifies cells undergoing DNA synthesis , was examined 2.5 hrs later. Fewer BrdU-positive cells were present in CaP of IkkαAA/AA/TRAMP...RNA synthesis are inhibited or in NF-κB-deficient cells. NF-κB exerts its pro-survival activity by induc- ing expression of several anti-apoptotic...new protein or RNA  synthesis  is inhibited or in NF-κB–deficient  cells. NF-κB exerts its pro-survival activity through several anti- apoptotic proteins

Intercomparison of planetary boundary layer parameterization and its impacts on surface ozone concentration in the WRF/Chem model for a case study in Houston/Texas

NASA Astrophysics Data System (ADS)

Cuchiara, G. C.; Li, X.; Carvalho, J.; Rappenglück, B.

2014-10-01

With over 6 million inhabitants the Houston metropolitan area is the fourth-largest in the United States. Ozone concentration in this southeast Texas region frequently exceeds the National Ambient Air Quality Standard (NAAQS). For this reason our study employed the Weather Research and Forecasting model with Chemistry (WRF/Chem) to quantify meteorological prediction differences produced by four widely used PBL schemes and analyzed its impact on ozone predictions. The model results were compared to observational data in order to identify one superior PBL scheme better suited for the area. The four PBL schemes include two first-order closure schemes, the Yonsei University (YSU) and the Asymmetric Convective Model version 2 (ACM2); as well as two turbulent kinetic energy closure schemes, the Mellor-Yamada-Janjic (MYJ) and Quasi-Normal Scale Elimination (QNSE). Four 24 h forecasts were performed, one for each PBL scheme. Simulated vertical profiles for temperature, potential temperature, relative humidity, water vapor mixing ratio, and the u-v components of the wind were compared to measurements collected during the Second Texas Air Quality Study (TexAQS-II) Radical and Aerosol Measurements Project (TRAMP) experiment in summer 2006. Simulated ozone was compared against TRAMP data, and air quality stations from Continuous Monitoring Station (CAMS). Also, the evolutions of the PBL height and vertical mixing properties within the PBL for the four simulations were explored. Although the results yielded high correlation coefficients and small biases in almost all meteorological variables, the overall results did not indicate any preferred PBL scheme for the Houston case. However, for ozone prediction the YSU scheme showed greatest agreements with observed values.

Intercomparison of Planetary Boundary Layer Parameterization and its Impacts on Surface Ozone Concentration in the WRF/Chem Model for a Case Study in Houston/Texas

NASA Astrophysics Data System (ADS)

Cuchiara, Gustavo C.; Li, Xiangshang; Carvalho, Jonas; Rappenglück, Bernhard

2015-04-01

With over 6 million inhabitants the Houston metropolitan area is the fourth-largest in the United States. Ozone concentration in this southeast Texas region frequently exceeds the National Ambient Air Quality Standard (NAAQS). For this reason our study employed the Weather Research and Forecasting model with Chemistry (WRF/Chem) to quantify meteorological prediction differences produced by four widely used PBL schemes and analyzed its impact on ozone predictions. The model results were compared to observational data in order to identify one superior PBL scheme better suited for the area. The four PBL schemes include two first-order closure schemes, the Yonsei University (YSU) and the Asymmetric Convective Model version 2 (ACM2); as well as two turbulent kinetic energy closure schemes, the Mellor-Yamada-Janjic (MYJ) and Quasi-Normal Scale Elimination (QNSE). Four 24 h forecasts were performed, one for each PBL scheme. Simulated vertical profiles for temperature, potential temperature, relative humidity, water vapor mixing ratio, and the u-v components of the wind were compared to measurements collected during the Second Texas Air Quality Study (TexAQS-II) Radical and Aerosol Measurements Project (TRAMP) experiment in summer 2006. Simulated ozone was compared against TRAMP data, and air quality stations from Continuous Monitoring Station (CAMS). Also, the evolutions of the PBL height and vertical mixing properties within the PBL for the four simulations were explored. Although the results yielded high correlation coefficients and small biases in almost all meteorological variables, the overall results did not indicate any preferred PBL scheme for the Houston case. However, for ozone prediction the YSU scheme showed greatest agreements with observed values.

Prostate Cancer Xenograft Inhibitory Activity and Pharmacokinetics of Decursinol, a Metabolite of Angelica gigas Pyranocoumarins, in Mouse Models.

PubMed

Wu, Wei; Tang, Su-Ni; Zhang, Yong; Puppala, Manohar; Cooper, Timothy K; Xing, Chengguo; Jiang, Cheng; Lü, Junxuan

2017-01-01

We have previously shown that the ethanol extract of dried Angelica gigas Nakai (AGN) root exerts anticancer activity against androgen receptor (AR)-negative human DU145 and PC-3 prostate cancer xenografts and primary carcinogenesis in the transgenic adenocarcinoma of mouse prostate (TRAMP) model. The major pyranocoumarin isomers decursin (D) and decursinol angelate (DA), when provided at equi-molar intake to that provided by AGN extract, accounted for the inhibitory efficacy against precancerous epithelial lesions in TRAMP mice. Since we and others have shown in rodents and humans that D and DA rapidly and extensively convert to decursinol, here we tested whether decursinol might be an in vivo active compound for suppressing xenograft growth of human prostate cancer cells expressing AR. In SCID-NSG mice carrying subcutaneously inoculated human LNCaP/AR-Luc cells overexpressing the wild type AR, we compared the efficacy of 4.5[Formula: see text]mg decursinol per mouse with equi-molar dose of 6[Formula: see text]mg D/DA per mouse. The result showed that decursinol decreased xenograft tumor growth by 75% and the lung metastasis, whereas D/DA exerted a much less effect. Measurement of plasma decursinol concentration, at 3[Formula: see text]h after the last dose of respective dosing regimen, showed higher circulating level in the decursinol-treated NSG mice than in the D/DA-treated mice. In a subsequent single-dose pharmacokinetic experiment, decursinol dosing led to 3.7-fold area under curve (AUC) of plasma decursinol over that achieved by equi-molar D/DA dosing. PK advantage notwithstanding, decursinol represents an active compound to exert in vivo prostate cancer growth and metastasis inhibitory activity in the preclinical model.

Prolonged exposure of resveratrol induces reactive superoxide species-independent apoptosis in murine prostate cells.

PubMed

Kumar, Sanjay; Stokes, James; Singh, Udai P; Scissum-Gunn, Karyn; Singh, Rajesh; Manne, Upender; Mishra, Manoj K

2017-10-01

Nitric oxide, a signaling molecule, inhibits mitochondrial respiration by binding with cytochrome c oxidase, resulting in elevated production of reactive superoxide species (reactive oxygen and nitrogen) in the mitochondria and increased susceptibility to cell death. Generation of mitochondrial superoxide species can be suppressed by natural compounds such as resveratrol, a dietary polyphenol found in the skin of red fruits. In various cancer cells, resveratrol shows anti-oxidant and cancer preventive properties. Since, the effect of resveratrol on reactive superoxide species-independent apoptosis in prostate cancer cells is not well illustrated; therefore, we investigated this phenomenon in TRAMP murine prostate cancer cells. To accomplish this, TRAMP cells were incubated with resveratrol, resveratrol + DETA-NONOate, DETA-NONOate (nitric oxide donor), resveratrol + L-NMMA, or L-NMMA (nitric oxide inhibitor) for 48 h, and reactive superoxide species in the mitochondria and culture supernatant were measured. In addition, the mitochondrial membrane potential, cell viability, expression of apoptotic markers (Bax and Bcl2), γ-H2A.x, p53, and caspase-3 was determined. We found that resveratrol suppressed reactive superoxide species such as reactive oxygen species in the mitochondria and nitric oxide in culture supernatant when compared to the DETA-NONOate treatment and disrupted the mitochondrial membrane potential. Resveratrol also reduced cell viability, altered the expression of apoptotic markers (Bax and Bcl2), and increased expression of γ-H2A.x (indicative marker of DNA fragmentation) and p53 (a critical DNA damage response protein). However, there was no appreciable modulation of the caspase-3. Therefore, our data suggest that resveratrol induces superoxide species-independent apoptosis and may act as a therapeutic agent against prostate cancer.

The Tunguska comet or the great Siberian mystery explosion of 1908

USGS Publications Warehouse

Spall, H.

1986-01-01

The boat disintegrates, hurling men and animals into the freezing river. The farmer's shirt is scorched off his back; the tramp is blown off his feet, his ports and pans scattering like shrapnel. One of the trappers standing in the doorway of the trading post is knocked unconscious, overcome by the searing heat; the tents of the nomads are torn loose, the men and dogs inside being rolled over the ground like leaves in a gale. All that remain of the reindeer are charred carcasses. The forest has been turning into a crackling inferno, and a black fireball rises into the air. 

On the Morphology of the Digestive System of Two Monomorium Ant Species

PubMed Central

Solis, Daniel Russ; Rossi, Mônica Lanzoni; Fox, Eduardo Gonçalves Paterson; Nogueira, Neusa de Lima; Tanaka, Francisco André Ossamu; Bueno, Odair Correa

2013-01-01

The digestive system of adults and mature larvae of two ant species of Monomorium Mayr (Hymoneptera: Formicidae) were described with the aid of light and scanning electron microscopy, as there is a lack of studies in this area. These two ant species are recurrently found in urban habitats and are known as ‘tramp species,’ as they cause problems in households, businesses, and hospitals. The most interesting finds of the present study include the existence of spinules in the crop of adults, and the number of Malpighian tubules and rectal pads was constant among different castes, ages, and species. PMID:24224520

A naive accelerometer acting in the continuum range.

PubMed

Peluso, F; Castagnolo, D; Albanese, C

2002-01-01

The space experiment TRAMP (Thermal Radiation Aspects of Migrating Particles) flown in 1999 onboard the mission Foton 12 sponsored by the European Space Agency (ESA), was conceived to reveal and measure a new kind of forces, named Thermal Radiation Forces (TRF). The experiment was dramatically disturbed by the occurrence of undesired convective motions due to the rotation of the spacecraft. Apart from that, corrosion occurred in some parts of the flight apparatus, resulting in the presence of gas bubbles inside the experimental liquid, completely compromising the results. Consequently, the experiment did not allow to reveal and/or to measure TRF, but it turned out to be useful in another way, as a very sensitive accelerometer, since the accelerations deduced from velocity measurements concurred with those measured by the Quasi-Steady Acceleration Measurement (QSAM) system.

Measurements of Intensive Aerosol Optical Properties During TexAQS II

NASA Astrophysics Data System (ADS)

Atkinson, D. B.; Radney, J. G.; Wright, M. E.

2007-12-01

Time-resolved measurements of the bulk extensive aerosol optical properties - particle extinction coefficient (bext) and particle scattering coefficient (bscat) - and particle number concentrations were made as part of the six-week TRAMP experiment during the TexAQS II (2006) study. These measurements were done at a nominal surface site (the roof of an 18 story building) on the University of Houston campus near downtown Houston, Texas. Our ground-based tandem cavity ring-down transmissometer/nephelometer instrument (CRDT/N) provided the aerosol optical property measurements. A commercial Condensation Particle Counter (TSI 3007) was used to measure the number concentrations during part of the study period. The optical data was used to construct the intensive aerosol optical properties single scattering albedo ω0 at 532 nm and the Angstrom exponent for extinction between 532 nm and 1064 nm. Recent validation studies of size- selected laboratory generated aerosols are presented to illustrate the soundness of this approach using our instrument. The Angstrom exponent is compared to values from other instruments operating in the area and is found to be a characteristic of the regional air mass under some conditions. Size distributions measured during the study were used to create a new empirical adjustment to scattering measured by the Radiance Research nephelometer, resulting in improved results for particle absorption coefficient and single scattering albedo. The study average value of ω0(532 nm) = 0.78 is lower than expected from comparable field studies and even lower values are experienced during the study. Possible causes of this discrepancy are examined and the utility of using the current version of the CRDT/N instrument to measure the key radiative property ω0 is assessed. Observed episodes of rapid increases in particle number concentration with little corresponding growth in the optical properties can presumably be used to signal the occurrence of particle nucleation or growth via gas-phase condensation. These results may be confirmed by other data taken during the TRAMP experiment. These results will be discussed in the context of aerosol effects on regional and larger scale climate.

Induction of Immune Mediators in Glioma and Prostate Cancer Cells by Non-Lethal Photodynamic Therapy

PubMed Central

Kammerer, Robert; Buchner, Alexander; Palluch, Patrick; Pongratz, Thomas; Oboukhovskij, Konstantin; Beyer, Wolfgang; Johansson, Ann; Stepp, Herbert; Baumgartner, Reinhold; Zimmermann, Wolfgang

2011-01-01

Background Photodynamic therapy (PDT) uses the combination of photosensitizing drugs and harmless light to cause selective damage to tumor cells. PDT is therefore an option for focal therapy of localized disease or for otherwise unresectable tumors. In addition, there is increasing evidence that PDT can induce systemic anti-tumor immunity, supporting control of tumor cells, which were not eliminated by the primary treatment. However, the effect of non-lethal PDT on the behavior and malignant potential of tumor cells surviving PDT is molecularly not well defined. Methodology/Principal Findings Here we have evaluated changes in the transcriptome of human glioblastoma (U87, U373) and human (PC-3, DU145) and murine prostate cancer cells (TRAMP-C1, TRAMP-C2) after non-lethal PDT in vitro and in vivo using oligonucleotide microarray analyses. We found that the overall response was similar between the different cell lines and photosensitizers both in vitro and in vivo. The most prominently upregulated genes encoded proteins that belong to pathways activated by cellular stress or are involved in cell cycle arrest. This response was similar to the rescue response of tumor cells following high-dose PDT. In contrast, tumor cells dealing with non-lethal PDT were found to significantly upregulate a number of immune genes, which included the chemokine genes CXCL2, CXCL3 and IL8/CXCL8 as well as the genes for IL6 and its receptor IL6R, which can stimulate proinflammatory reactions, while IL6 and IL6R can also enhance tumor growth. Conclusions Our results indicate that PDT can support anti-tumor immune responses and is, therefore, a rational therapy even if tumor cells cannot be completely eliminated by primary phototoxic mechanisms alone. However, non-lethal PDT can also stimulate tumor growth-promoting autocrine loops, as seen by the upregulation of IL6 and its receptor. Thus the efficacy of PDT to treat tumors may be improved by controlling unwanted and potentially deleterious growth-stimulatory pathways. PMID:21738796

Low-cost detection of RC-IED activation signals in VHF band

NASA Astrophysics Data System (ADS)

Camargo Suarez, Victor Hugo; Marulanda B., Jose Ignacio

2014-05-01

The proliferation of Radio Controlled Improvised Explosive Devices (RC-IED) is a growing threat around the world. The ease of construction and low cost of these devices are transforming common things in lethal tramps. The fight against this threats normally involves the use of sophisticated and expensive equipment of Electronic Warfare based on high speed DSP systems, just to detect the presence of detonation signals. In this work is showed how to find activation signals based on the characteristic of the power in a specific band and the previous knowledge about the detonation signals. As proof of concept we have taken the information about the RC-IEDs used in the Colombian conflict and develop an algorithm to find detonation signals based on the measured power in frequencies between 136 MHz and 174 MHz (2 meter civil band)

How safe is adventure tourism in New Zealand? An exploratory analysis.

PubMed

Bentley, T; Page, S; Meyer, D; Chalmers, D; Laird, I

2001-08-01

The paper reports findings from a multidisciplinary programme of research, the major aims of which were to determine the nature and extent of the New Zealand adventure tourism injury problem. Analysis of hospital discharge and mortality data for a 15-year period identified adventure tourism-related activities as contributing to approximately 20% of overseas visitor injuries, and 22% of fatalities. Activities that commonly involve independent-unguided adventure tourism, notably mountaineering, skiing and tramping, contributed most to injury and fatality incidence. Horse riding and cycling activities were identified from hospital discharge data and adventure tourism operators' reported client injury-incidence, as the commercial adventure tourism activities most frequently involved in client injuries. Falls were the most common injury events, and a range of client, equipment, environmental and organisational risk factors were identified. Possible interventions to reduce injury risk among overseas and domestic adventure tourists are discussed.

Hydrodynamic Studies of Turbulent AGN Tori

NASA Astrophysics Data System (ADS)

Schartmann, M.; Meisenheimer, K.; Klahr, H.; Camenzind, M.; Wolf, S.; Henning, Th.; Burkert, A.; Krause, M.

Recently, the MID-infrared Interferometric instrument (MIDI) at the VLTI has shown that dust tori in the two nearby Seyfert galaxies NGC 1068 and the Circinus galaxy are geometrically thick and can be well described by a thin, warm central disk, surrounded by a colder and fluffy torus component. By carrying out hydrodynamical simulations with the help of the TRAMP code (Klahr et al. 1999), we follow the evolution of a young nuclear star cluster in terms of discrete mass-loss and energy injection from stellar processes. This naturally leads to a filamentary large scale torus component, where cold gas is able to flow radially inwards. The filaments join into a dense and very turbulent disk structure. In a post-processing step, we calculate spectral energy distributions and images with the 3D radiative transfer code MC3D Wolf (2003) and compare them to observations. Turbulence in the dense disk component is investigated in a separate project.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Muth, Thomas R; Yamamoto, Yukinori; Frederick, David Alan

ORNL undertook an investigation using gas tungsten arc (GTA) welding on consolidated powder metallurgy (PM) titanium (Ti) plate, to identify the causal factors behind observed porosity in fusion welding. Tramp element compounds of sodium and magnesium, residual from the metallothermic reduction of titanium chloride used to produce the titanium, were remnant in the starting powder and were identified as gas forming species. PM-titanium made from revert scrap where sodium and magnesium were absent, showed fusion weld porosity, although to a lesser degree. We show that porosity was attributable to hydrogen from adsorbed water on the surface of the powders priormore » to consolidation. The removal / minimization of both adsorbed water on the surface of titanium powder and the residues from the reduction process prior to consolidation of titanium powders, are critical to achieve equivalent fusion welding success similar to that seen in wrought titanium produced via the Kroll process.« less

Causal Factors of Weld Porosity in Gas Tungsten Arc Welding of Powder-Metallurgy-Produced Titanium Alloys

NASA Astrophysics Data System (ADS)

Muth, T. R.; Yamamoto, Y.; Frederick, D. A.; Contescu, C. I.; Chen, W.; Lim, Y. C.; Peter, W. H.; Feng, Z.

2013-05-01

An investigation was undertaken using gas tungsten arc (GTA) welding on consolidated powder metallurgy (PM) titanium (Ti) plate to identify the causal factors behind observed porosity in fusion welding. Tramp element compounds of sodium and magnesium, residual from the metallothermic reduction of titanium chloride used to produce the titanium, were remnant in the starting powder and were identified as gas-forming species. PM-titanium made from revert scrap, where sodium and magnesium were absent, showed fusion weld porosity, although to a lesser degree. We show that porosity was attributable to hydrogen from adsorbed water on the surface of the powders prior to consolidation. The removal and minimization of both adsorbed water on the surface of titanium powder and the residues from the reduction process prior to consolidation of titanium powders are critical for achieving equivalent fusion welding success similar to that seen in wrought titanium produced via the Kroll process.

Mangiferin Has an Additive Effect on the Apoptotic Properties of Hesperidin in Cyclopia sp. Tea Extracts

PubMed Central

Bartoszewski, Rafal; Hering, Anna; Marszałł, Marcin; Stefanowicz Hajduk, Justyna; Bartoszewska, Sylwia; Kapoor, Niren; Kochan, Kinga; Ochocka, Renata

2014-01-01

A variety of biological pro-health activities have been reported for mangiferin and hesperidin, two major phenolic compounds of Honeybush (Cyclopia sp.) tea extracts. Given their increasing popularity, there is a need for understanding the mechanisms underlying the biological effects of these compounds. In this study, we used real-time cytotoxicity cellular analysis of the Cyclopia sp. extracts on HeLa cells and found that the higher hesperidin content in non-fermented "green" extracts correlated with their higher cytotoxicity compared to the fermented extracts. We also found that mangiferin had a modulatory effect on the apoptotic effects of hesperidin. Quantitative PCR analysis of hesperidin-induced changes in apoptotic gene expression profile indicated that two death receptor pathway members, TRADD and TRAMP, were up regulated. The results of this study suggest that hesperidin mediates apoptosis in HeLa cells through extrinsic pathway for programmed cell death. PMID:24633329

The refining of secondary lead for use in advanced lead-acid batteries

NASA Astrophysics Data System (ADS)

Ellis, Timothy W.; Mirza, Abbas H.

Secondary lead, i.e. material produced by the recycling of lead-acid batteries has become the primary source of lead in much of the world. This has been important to the secondary lead industry as other uses have dwindled, e.g. lead based pigments, chemicals, fuel additives, solders and CRT glasses [1]. Presently, battery manufacturing accounts for greater than 80% of lead consumption while recycled lead accounts for approximately the same market share of lead supply. These two facts strongly demonstrate the battery manufacturing and recycled lead are intimately coupled in everyday life. In this paper we will explore how recycled lead has become the material of choice for battery construction through the development of a recovery and refining process that exceeds the industries requirements. Particular focus will be on addressing the results presented by Prengaman [2] on the effects of contaminant or tramp elements on gassing in lead-acid batteries.

Reactive stroma in the prostate during late life: The role of microvasculature and antiangiogenic therapy influences.

PubMed

Montico, Fabio; Kido, Larissa Akemi; San Martin, Rebeca; Rowley, David R; Cagnon, Valéria H A

2015-10-01

Prostate cancer is associated to a reactive stroma microenvironment characterized by angiogenic processes that are favorable for tumor progression. Senescence has been identified as a predisposing factor for prostate malignancies. In turn, the relationships between aging, reactive stroma, and the mechanisms that induce this phenotype are largely unknown. Thus, we investigated the occurrence of reactive stroma in the mouse prostate during advanced age as well as the effects of antiangiogenic and androgen ablation therapies on reactive stroma recruitment. Male mice (52-week-old FVB) were treated with two classes of angiogenesis inhibitors: direct (TNP-470; 15 mg/kg; s.c.) and/or indirect (SU5416; 6 mg/kg; i.p.). Androgen ablation was carried out by finasteride administration (20 mg/kg; s.c.), alone or in association to both inhibitors. The Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP) model was used as a paradigm of cancer-associated reactive stroma. The dorsolateral prostate was collected for α-actin (αSMA), vimentin (VIM), and transforming growth factor-beta (TGF-β) immunohistochemical and Western blotting analyses as well as for CD34/αSMA and CD34/VIM colocalization. Senescence was associated with increased αSMA, VIM, and TGF-β expression as well as with the recruitment of CD34/αSMA and CD34/VIM dual-positive fibroblasts. These observations were similar to those verified in TRAMP mice. Antiangiogenic treatment promoted the recovery of senescence-associated stromal changes. Hormonal ablation, despite having led to impaired CD34/αSMA and CD34/VIM dual-positive cell recruitment, did not result in decreased stimulus to reactive stroma development, due to enhanced TGF-β expression in relation to the aged controls. Reactive stroma develops in the prostate of non-transgenic mice as a result of aging. The periacinar microvasculature is a candidate source for the recruitment of reactive stroma-associated cells, which may be derived either from perivascular-resident mesenchymal stem cells (MSCs) or from an endothelial-to-mesenchymal transition (EndMT) process. Thus, antiangiogenic therapy is a promising approach for preventing age-associated prostate malignancies by means of its negative interference in the development of reactive stroma phenotype from the vascular wall. © 2015 Wiley Periodicals, Inc.

46 CFR 42.05-63 - Ship(s) and vessel(s).

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Ship(s) and vessel(s). 42.05-63 Section 42.05-63 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES DOMESTIC AND FOREIGN VOYAGES BY SEA Definition of Terms Used in This Subchapter § 42.05-63 Ship(s) and vessel(s). The terms ship(s...

46 CFR 42.05-63 - Ship(s) and vessel(s).

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Ship(s) and vessel(s). 42.05-63 Section 42.05-63 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES DOMESTIC AND FOREIGN VOYAGES BY SEA Definition of Terms Used in This Subchapter § 42.05-63 Ship(s) and vessel(s). The terms ship(s...

46 CFR 42.05-63 - Ship(s) and vessel(s).

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Ship(s) and vessel(s). 42.05-63 Section 42.05-63 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES DOMESTIC AND FOREIGN VOYAGES BY SEA Definition of Terms Used in This Subchapter § 42.05-63 Ship(s) and vessel(s). The terms ship(s...

46 CFR 42.05-63 - Ship(s) and vessel(s).

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Ship(s) and vessel(s). 42.05-63 Section 42.05-63 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES DOMESTIC AND FOREIGN VOYAGES BY SEA Definition of Terms Used in This Subchapter § 42.05-63 Ship(s) and vessel(s). The terms ship(s...

46 CFR 42.05-63 - Ship(s) and vessel(s).

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Ship(s) and vessel(s). 42.05-63 Section 42.05-63... BY SEA Definition of Terms Used in This Subchapter § 42.05-63 Ship(s) and vessel(s). The terms ship(s) and vessel(s) are interchangeable or synonymous words, and include every description of watercraft...

47 CFR 80.1121 - Receipt and acknowledgement of distress alerts by ship stations and ship earth stations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... by ship stations and ship earth stations. 80.1121 Section 80.1121 Telecommunication FEDERAL... § 80.1121 Receipt and acknowledgement of distress alerts by ship stations and ship earth stations. (a) Ship or ship earth stations that receive a distress alert must, as soon as possible, inform the master...

47 CFR 80.1121 - Receipt and acknowledgement of distress alerts by ship stations and ship earth stations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... by ship stations and ship earth stations. 80.1121 Section 80.1121 Telecommunication FEDERAL... § 80.1121 Receipt and acknowledgement of distress alerts by ship stations and ship earth stations. (a) Ship or ship earth stations that receive a distress alert must, as soon as possible, inform the master...

47 CFR 80.1121 - Receipt and acknowledgement of distress alerts by ship stations and ship earth stations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... by ship stations and ship earth stations. 80.1121 Section 80.1121 Telecommunication FEDERAL... § 80.1121 Receipt and acknowledgement of distress alerts by ship stations and ship earth stations. (a) Ship or ship earth stations that receive a distress alert must, as soon as possible, inform the master...

47 CFR 80.1121 - Receipt and acknowledgement of distress alerts by ship stations and ship earth stations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... by ship stations and ship earth stations. 80.1121 Section 80.1121 Telecommunication FEDERAL... § 80.1121 Receipt and acknowledgement of distress alerts by ship stations and ship earth stations. (a) Ship or ship earth stations that receive a distress alert must, as soon as possible, inform the master...

47 CFR 80.1121 - Receipt and acknowledgement of distress alerts by ship stations and ship earth stations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... by ship stations and ship earth stations. 80.1121 Section 80.1121 Telecommunication FEDERAL... § 80.1121 Receipt and acknowledgement of distress alerts by ship stations and ship earth stations. (a) Ship or ship earth stations that receive a distress alert must, as soon as possible, inform the master...

Androgen deprivation and stem cell markers in prostate cancers

PubMed Central

Tang, Yao; Hamburger, Anne W; Wang, Linbo; Khan, Mohammad Afnan; Hussain, Arif

2010-01-01

In our previous studies using human LNCaP xenografts and TRAMP (transgenic adenocarcinoma of mouse prostate) mice, androgen deprivation therapy (ADT) resulted in a temporary cessation of prostate cancer (PCa) growth, but then tumors grew faster with more malignant behaviour. To understand whether cancer stem cells might play a role in PCa progression in these animal models, we investigated the expressions of stem cell-related markers in tumors at different time points after ADT. In both animal models, enhanced expressions of stem cell markers were observed in tumors of castrated mice, as compared to non-castrated controls. This increased cell population that expressed stem cell markers is designated as stem-like cells (SLC) in this article. We also observed that the SLC peaked at relatively early time points after ADT, before tumors resumed their growth. These results suggest that the SLC population may play a role in tumor re-growth and disease progression, and that targeting the SLC at their peak-expression time point may prevent tumor recurrence following ADT. PMID:20126580

The activity of calcium in calcium-metal-fluoride fluxes

NASA Astrophysics Data System (ADS)

Ochifuji, Yuichiro; Tsukihashi, Fumitaka; Sano, Nobuo

1995-08-01

The standard Gibbs energy of reaction Ca (1) + O (mass pct, in Zr) = CaO (s) has been determined as follows by equilibrating molten calcium with solid zirconium in a CaO crucible: Δ G° = -64,300(±700) + 19.8(±3.5) T J/mol (1373 to 1623 K) The activities of calcium in the CaOsatd-Ca- MF2 ( M: Ca, Ba, Mg) and CaOsatd-Ca-NaF systems were measured as a function of calcium composition at high calcium contents at 1473 K on the basis of the standard Gibbs energy. The activities of calcium increase in the order of CaF2, BaF2, and MgF2 at the same calcium fraction of these fluxes. The observed activities are compared with those estimated by using the Temkin model for ionic solutions. Furthermore, the possibility of the removal of tramp elements such as tin, arsenic, antimony, bismuth, and lead from carbon-saturated iron by using calcium-metal-fluoride fluxes is discussed.

The Impact of Ship-Produced Aerosols on the Microstructure and Albedo of Warm Marine Stratocumulus Clouds: A Test of MAST Hypotheses 1i and 1ii.

NASA Astrophysics Data System (ADS)

Durkee, P. A.; Noone, K. J.; Ferek, R. J.; Johnson, D. W.; Taylor, J. P.; Garrett, T. J.; Hobbs, P. V.; Hudson, J. G.; Bretherton, C. S.; Innis, G.; Frick, G. M.; Hoppel, W. A.; O'Dowd, C. D.; Russell, L. M.; Gasparovic, R.; Nielsen, K. E.; Tessmer, S. A.; Öström, E.;  Osborne, S. R.;  Flagan, R. C.;  Seinfeld, J. H.;  Rand, H.

2000-08-01

Anomalously high reflectivity tracks in stratus and stratocumulus sheets associated with ships (known as ship tracks) are commonly seen in visible and near-infrared satellite imagery. Until now there have been only a limited number of in situ measurements made in ship tracks. The Monterey Area Ship Track (MAST) experiment, which was conducted off the coast of California in June 1994, provided a substantial dataset on ship emissions and their effects on boundary layer clouds. Several platforms, including the University of Washington C-131A aircraft, the Meteorological Research Flight C-130 aircraft, the National Aeronautics and Space Administration ER-2 aircraft, the Naval Research Laboratory airship, the Research Vessel Glorita, and dedicated U.S. Navy ships, participated in MAST in order to study processes governing the formation and maintenance of ship tracks.This paper tests the hypotheses that the cloud microphysical changes that produce ship tracks are due to (a) particulate emission from the ship's stack and/or (b) sea-salt particles from the ship's wake. It was found that ships powered by diesel propulsion units that emitted high concentrations of aerosols in the accumulation mode produced ship tracks. Ships that produced few particles (such as nuclear ships), or ships that produced high concentrations of particles but at sizes too small to be activated as cloud drops in typical stratocumulus (such as gas turbine and some steam-powered ships), did not produce ship tracks. Statistics and case studies, combined with model simulations, show that provided a cloud layer is susceptible to an aerosol perturbation, and the atmospheric stability enables aerosol to be mixed throughout the boundary layer, the direct emissions of cloud condensation nuclei from the stack of a diesel-powered ship is the most likely, if not the only, cause of the formation of ship tracks. There was no evidence that salt particles from ship wakes cause ship tracks.

46 CFR 167.05-25 - Nautical school ship.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Nautical school ship. 167.05-25 Section 167.05-25 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS PUBLIC NAUTICAL SCHOOL SHIPS Definitions § 167.05-25 Nautical school ship. The term nautical school ship means a vessel...

46 CFR 167.05-25 - Nautical school ship.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Nautical school ship. 167.05-25 Section 167.05-25 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS PUBLIC NAUTICAL SCHOOL SHIPS Definitions § 167.05-25 Nautical school ship. The term nautical school ship means a vessel...

46 CFR 167.05-25 - Nautical school ship.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Nautical school ship. 167.05-25 Section 167.05-25 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS PUBLIC NAUTICAL SCHOOL SHIPS Definitions § 167.05-25 Nautical school ship. The term nautical school ship means a vessel...

46 CFR Sec. 5 - Measures to protect ship's payrolls.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 8 2012-10-01 2012-10-01 false Measures to protect ship's payrolls. Sec. 5 Section 5 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY BONDING OF SHIP'S PERSONNEL Sec. 5 Measures to protect ship's payrolls. (a) General Agents are not required to...

46 CFR 167.05-25 - Nautical school ship.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Nautical school ship. 167.05-25 Section 167.05-25 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS PUBLIC NAUTICAL SCHOOL SHIPS Definitions § 167.05-25 Nautical school ship. The term nautical school ship means a vessel...

46 CFR 167.05-25 - Nautical school ship.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Nautical school ship. 167.05-25 Section 167.05-25 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS PUBLIC NAUTICAL SCHOOL SHIPS Definitions § 167.05-25 Nautical school ship. The term nautical school ship means a vessel...

The inositol phosphatase SHIP-2 down-regulates FcγR-mediated phagocytosis in murine macrophages independently of SHIP-1

PubMed Central

Ai, Jing; Maturu, Amita; Johnson, Wesley; Wang, Yijie; Marsh, Clay B.; Tridandapani, Susheela

2006-01-01

FcγR-mediated phagocytosis of IgG-coated particles is a complex process involving the activation of multiple signaling enzymes and is regulated by the inositol phosphatases PTEN (phosphatase and tensin homolog deleted on chromosome 10) and SHIP-1 (Src homology [SH2] domain-containing inositol phosphatase). In a recent study we have demonstrated that SHIP-2, an inositol phosphatase with high-level homology to SHIP-1, is involved in FcγR signaling. However, it is not known whether SHIP-2 plays a role in modulating phagocytosis. In this study we have analyzed the role of SHIP-2 in FcγR-mediated phagocytosis using independent cell models that allow for manipulation of SHIP-2 function without influencing the highly homologous SHIP-1. We present evidence that SHIP-2 translocates to the site of phagocytosis and down-regulates FcγR-mediated phagocytosis. Our data indicate that SHIP-2 must contain both the N-terminal SH2 domain and the C-terminal proline-rich domain to mediate its inhibitory effect. The effect of SHIP-2 is independent of SHIP-1, as overexpression of dominant-negative SHIP-2 in SHIP-1-deficient primary macrophages resulted in enhanced phagocytic efficiency. Likewise, specific knockdown of SHIP-2 expression using siRNA resulted in enhanced phagocytosis. Finally, analysis of the molecular mechanism of SHIP-2 down-regulation of phagocytosis revealed that SHIP-2 down-regulates upstream activation of Rac. Thus, we conclude that SHIP-2 is a novel negative regulator of FcγR-mediated phagocytosis independent of SHIP-1. (Blood. 2006;107:813-820) PMID:16179375

Spatial pattern analysis of cruise ship-humpback whale interactions in and near Glacier Bay National Park, Alaska.

PubMed

Harris, Karin; Gende, Scott M; Logsdon, Miles G; Klinger, Terrie

2012-01-01

Understanding interactions between large ships and large whales is important to estimate risks posed to whales by ships. The coastal waters of Alaska are a summer feeding area for humpback whales (Megaptera novaeangliae) as well as a prominent destination for large cruise ships. Lethal collisions between cruise ships and humpback whales have occurred throughout Alaska, including in Glacier Bay National Park (GBNP). Although the National Park Service (NPS) establishes quotas and operating requirements for cruise ships within GBNP in part to minimize ship-whale collisions, no study has quantified ship-whale interactions in the park or in state waters where ship traffic is unregulated. In 2008 and 2009, an observer was placed on ships during 49 different cruises that included entry into GBNP to record distance and bearing of whales that surfaced within 1 km of the ship's bow. A relative coordinate system was developed in ArcGIS to model the frequency of whale surface events using kernel density. A total of 514 whale surface events were recorded. Although ship-whale interactions were common within GBNP, whales frequently surfaced in front of the bow in waters immediately adjacent to the park (west Icy Strait) where cruise ship traffic is not regulated by the NPS. When ships transited at speeds >13 knots, whales frequently surfaced closer to the ship's midline and ship's bow in contrast to speeds slower than 13 knots. Our findings confirm that ship speed is an effective mitigation measure for protecting whales and should be applied to other areas where ship-whale interactions are common.

Spatial Pattern Analysis of Cruise Ship-Humpback Whale Interactions in and Near Glacier Bay National Park, Alaska

NASA Astrophysics Data System (ADS)

Harris, Karin; Gende, Scott M.; Logsdon, Miles G.; Klinger, Terrie

2012-01-01

Understanding interactions between large ships and large whales is important to estimate risks posed to whales by ships. The coastal waters of Alaska are a summer feeding area for humpback whales ( Megaptera novaeangliae) as well as a prominent destination for large cruise ships. Lethal collisions between cruise ships and humpback whales have occurred throughout Alaska, including in Glacier Bay National Park (GBNP). Although the National Park Service (NPS) establishes quotas and operating requirements for cruise ships within GBNP in part to minimize ship-whale collisions, no study has quantified ship-whale interactions in the park or in state waters where ship traffic is unregulated. In 2008 and 2009, an observer was placed on ships during 49 different cruises that included entry into GBNP to record distance and bearing of whales that surfaced within 1 km of the ship's bow. A relative coordinate system was developed in ArcGIS to model the frequency of whale surface events using kernel density. A total of 514 whale surface events were recorded. Although ship-whale interactions were common within GBNP, whales frequently surfaced in front of the bow in waters immediately adjacent to the park (west Icy Strait) where cruise ship traffic is not regulated by the NPS. When ships transited at speeds >13 knots, whales frequently surfaced closer to the ship's midline and ship's bow in contrast to speeds slower than 13 knots. Our findings confirm that ship speed is an effective mitigation measure for protecting whales and should be applied to other areas where ship-whale interactions are common.

Vertical profiles of nitrous acid in the nocturnal urban atmosphere of Houston, TX

NASA Astrophysics Data System (ADS)

Wong, K. W.; Oh, H.-J.; Lefer, B.; Rappenglück, B.; Stutz, J.

2010-12-01

Nitrous acid (HONO) often plays an important role in tropospheric photochemistry as a major precursor of the hydroxyl radical (OH) in early morning hours and potentially during the day. However, the processes leading to formation of HONO and its vertical distribution at night, which can have a considerable impact on daytime ozone formation, are currently poorly characterized by observations and models. Long-path differential optical absorption spectroscopy (LP-DOAS) measurements of HONO during the 2006 TexAQS II Radical and Aerosol Measurement Project (TRAMP), near downtown Houston, TX, show nocturnal vertical profiles of HONO, with mixing ratios of up to 2.2 ppb near the surface and below 100 ppt aloft. Three nighttime periods of HONO, NO2 and O3 observations during TRAMP were used to perform model simulations of vertical mixing ratio profiles. By adjusting vertical mixing and NOx emissions the modeled NO2 and O3 mixing ratios showed very good agreement with the observations. Using a simple conversion of NO2 to HONO on the ground, direct HONO emissions, as well as HONO loss at the ground and on aerosol, the observed HONO profiles were reproduced well by the model. The unobserved increase of HONO to NO2 ratio (HONO/NO2) with altitude that was simulated by the initial model runs was found to be due to HONO uptake being too small on aerosol and too large on the ground. Refined model runs, with adjusted HONO uptake coefficients, showed much better agreement of HONO and HONO/NO2 for two typical nights, except during morning rush hour, when other HONO formation pathways are most likely active. One of the nights analyzed showed increase of HONO mixing ratios together with decreasing NO2 mixing ratios that the model was unable to reproduce, most likely due to the impact of weak precipitation during this night. HONO formation and removal rates averaged over the lowest 300 m of the atmosphere showed that NO2 to HONO conversion on the ground was the dominant source of HONO, followed by traffic emission. Aerosol did not play an important role in HONO formation. Although ground deposition was also a major removal pathway of HONO, net HONO production at the ground was the main source of HONO in our model studies. Sensitivity studies showed that in the stable NBL, net HONO production at the ground tends to increase with faster vertical mixing and stronger emission. Vertical transport was found to be the dominant source of HONO aloft.

The computer coordination method and research of inland river traffic based on ship database

NASA Astrophysics Data System (ADS)

Liu, Shanshan; Li, Gen

2018-04-01

A computer coordinated management method for inland river ship traffic is proposed in this paper, Get the inland ship's position, speed and other navigation information by VTS, building ship's statics and dynamic data bases, writing a program of computer coordinated management of inland river traffic by VB software, Automatic simulation and calculation of the meeting states of ships, Providing ship's long-distance collision avoidance information. The long-distance collision avoidance of ships will be realized. The results show that, Ships avoid or reduce meetings, this method can effectively control the macro collision avoidance of ships.

46 CFR Sec. 19 - Ship Repair Summaries.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 8 2012-10-01 2012-10-01 false Ship Repair Summaries. Sec. 19 Section 19 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP...

46 CFR Sec. 19 - Ship Repair Summaries.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Ship Repair Summaries. Sec. 19 Section 19 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP...

46 CFR Sec. 19 - Ship Repair Summaries.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Ship Repair Summaries. Sec. 19 Section 19 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP...

46 CFR Sec. 19 - Ship Repair Summaries.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 8 2013-10-01 2013-10-01 false Ship Repair Summaries. Sec. 19 Section 19 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP...

46 CFR Sec. 19 - Ship Repair Summaries.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Ship Repair Summaries. Sec. 19 Section 19 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY PROCEDURE FOR ACCOMPLISHMENT OF VESSEL REPAIRS UNDER NATIONAL SHIPPING AUTHORITY MASTER LUMP SUM REPAIR CONTRACT-NSA-LUMPSUMREP...

Shipping emissions and their impacts on air quality in China.

PubMed

Zhang, Yan; Yang, Xin; Brown, Richard; Yang, Liping; Morawska, Lidia; Ristovski, Zoran; Fu, Qingyan; Huang, Cheng

2017-03-01

China has >400 ports, is home to 7 of 10 biggest ports in the world and its waterway infrastructure construction has been accelerating over the past years. But the increasing number of ports and ships means increasing emissions, and in turn, increasing impact on local and regional air pollution. This paper presents an overview of the broad field of ship emissions in China and their atmospheric impacts, including topics of ship engine emissions and control, ship emission factors and their measurements, developing of ship emission inventories, shipping and port emissions of the main shipping areas in China, and quantitative contribution of shipping emissions to the local and regional air pollution. There have been an increasing number of studies published on all the above aspects, yet, this review identified some critical research gaps, filling of which is necessary for better control of ship emissions, and for lowering their impacts. In particular, there are very few studies on inland ports and river ships, and there are few national scale ship emission inventories available for China. While advanced method to estimate ship emission based on ship AIS activities makes it now possible to develop high spatial- and temporal-resolution emission inventories, the ship emission factors used in Chinese studies have been based mainly on foreign measurements. Further, the contribution of ship emissions to air pollution in coastal cities, the dispersion of pollution plumes emitted by ships, or the chemical evolution process along the transmission path, have so far not been systematically studied in China. Copyright © 2016 Elsevier B.V. All rights reserved.

48 CFR 1371.118 - Changes-ship repair.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Changes-ship repair. 1371... SUPPLEMENTAL REGULATIONS ACQUISITIONS INVOLVING SHIP CONSTRUCTION AND SHIP REPAIR Provisions and Clauses 1371.118 Changes—ship repair. Insert clause 1352.271-87, Changes—Ship Repair, in all solicitations and...

48 CFR 1371.118 - Changes-ship repair.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Changes-ship repair. 1371... SUPPLEMENTAL REGULATIONS ACQUISITIONS INVOLVING SHIP CONSTRUCTION AND SHIP REPAIR Provisions and Clauses 1371.118 Changes—ship repair. Insert clause 1352.271-87, Changes—Ship Repair, in all solicitations and...

48 CFR 1371.118 - Changes-ship repair.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Changes-ship repair. 1371... SUPPLEMENTAL REGULATIONS ACQUISITIONS INVOLVING SHIP CONSTRUCTION AND SHIP REPAIR Provisions and Clauses 1371.118 Changes—ship repair. Insert clause 1352.271-87, Changes—Ship Repair, in all solicitations and...

48 CFR 1371.118 - Changes-ship repair.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Changes-ship repair. 1371... SUPPLEMENTAL REGULATIONS ACQUISITIONS INVOLVING SHIP CONSTRUCTION AND SHIP REPAIR Provisions and Clauses 1371.118 Changes—ship repair. Insert clause 1352.271-87, Changes—Ship Repair, in all solicitations and...

Lift-based up-ender and methods using same to manipulate a shipping container containing unirradiated nuclear fuel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nilles, Michael J.

A shipping container containing an unirradiated nuclear fuel assembly is lifted off the ground by operating a crane to raise a lifting tool comprising a winch. The lifting tool is connected with the shipping container by a rigging line connecting with the shipping container at a lifting point located on the shipping container between the top and bottom of the shipping container, and by winch cabling connecting with the shipping container at the top of the shipping container. The shipping container is reoriented by operating the winch to adjust the length of the winch cabling so as to rotate themore » shipping container about the lifting point. Shortening the winch cabling rotates the shipping container about the lifting point from a horizontal orientation to a vertical orientation, while lengthening the winch cabling rotates the shipping container about the lifting point from the vertical orientation to the horizontal orientation.« less

46 CFR 153.7 - Ships built before December 27, 1977 and non-self-propelled ships built before July 1, 1983...

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Ships built before December 27, 1977 and non-self-propelled ships built before July 1, 1983: Application. 153.7 Section 153.7 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS...

Speckle noise reduction in SAR images ship detection

NASA Astrophysics Data System (ADS)

Yuan, Ji; Wu, Bin; Yuan, Yuan; Huang, Qingqing; Chen, Jingbo; Ren, Lin

2012-09-01

At present, there are two types of method to detect ships in SAR images. One is a direct detection type, detecting ships directly. The other is an indirect detection type. That is, it firstly detects ship wakes, and then seeks ships around wakes. The two types all effect by speckle noise. In order to improve the accuracy of ship detection and get accurate ship and ship wakes parameters, such as ship length, ship width, ship area, the angle of ship wakes and ship outline from SAR images, it is extremely necessary to remove speckle noise in SAR images before data used in various SAR images ship detection. The use of speckle noise reduction filter depends on the specification for a particular application. Some common filters are widely used in speckle noise reduction, such as the mean filter, the median filter, the lee filter, the enhanced lee filter, the Kuan filter, the frost filter, the enhanced frost filter and gamma filter, but these filters represent some disadvantages in SAR image ship detection because of the various types of ship. Therefore, a mathematical function known as the wavelet transform and multi-resolution analysis were used to localize an SAR ocean image into different frequency components or useful subbands, and effectively reduce the speckle in the subbands according to the local statistics within the bands. Finally, the analysis of the statistical results are presented, which demonstrates the advantages and disadvantages of using wavelet shrinkage techniques over standard speckle filters.

Radiated noise from commercial ships in the Gulf of Maine: implications for whale/vessel collisions.

PubMed

Allen, J Kaitlyn; Peterson, Michael L; Sharrard, George V; Wright, Dana L; Todd, Sean K

2012-09-01

To understand mysticete acoustic-based detection of ships, radiated noise from high-speed craft, cruise ships, catamarans and fishing vessels was recorded June-September 2009. Calibrated acoustic data (<2500 Hz) from a vertical hydrophone array was combined with ship passage information. A cruise ship had the highest broadband source level, while a fishing vessel had the lowest. Ship noise radiated asymmetrically and varied with depth. Bow null-effect acoustic shadow zones were observed for all ship classes and were correlated with ship-length-to-draft-ratios. These shadow zones may reduce ship detection by near-surface mysticetes.

7 CFR 984.472 - Reports of merchantable walnuts shipped.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 8 2011-01-01 2011-01-01 false Reports of merchantable walnuts shipped. 984.472... walnuts shipped. (a) Reports of merchantable walnuts shipped during a month shall be submitted to the... shipped; whether they were shipped into domestic or export channels; and for exports, the quantity by...

47 CFR 80.1083 - Ship radio installations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Ship radio installations. 80.1083 Section 80... for Ship Stations § 80.1083 Ship radio installations. (a) Ships must be provided with radio... controls for operating the radio installation; and (5) Be clearly marked with the call sign, the ship...

7 CFR 984.472 - Reports of merchantable walnuts shipped.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 8 2012-01-01 2012-01-01 false Reports of merchantable walnuts shipped. 984.472... walnuts shipped. (a) Reports of merchantable walnuts shipped during a month shall be submitted to the... shipped; whether they were shipped into domestic or export channels; and for exports, the quantity by...

47 CFR 80.1085 - Ship radio equipment-General.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Ship radio equipment-General. 80.1085 Section... Requirements for Ship Stations § 80.1085 Ship radio equipment—General. This section contains the general equipment requirements for all ships subject to this subpart. (a) Ships must be provided with: (1) A VHF...

7 CFR 984.472 - Reports of merchantable walnuts shipped.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 8 2013-01-01 2013-01-01 false Reports of merchantable walnuts shipped. 984.472... walnuts shipped. (a) Reports of merchantable walnuts shipped during a month shall be submitted to the... shipped; whether they were shipped into domestic or export channels; and for exports, the quantity by...

7 CFR 984.472 - Reports of merchantable walnuts shipped.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 8 2014-01-01 2014-01-01 false Reports of merchantable walnuts shipped. 984.472... walnuts shipped. (a) Reports of merchantable walnuts shipped during a month shall be submitted to the... shipped; whether they were shipped into domestic or export channels; and for exports, the quantity by...

47 CFR 80.1085 - Ship radio equipment-General.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 5 2013-10-01 2013-10-01 false Ship radio equipment-General. 80.1085 Section... Requirements for Ship Stations § 80.1085 Ship radio equipment—General. This section contains the general equipment requirements for all ships subject to this subpart. (a) Ships must be provided with: (1) A VHF...

47 CFR 80.1085 - Ship radio equipment-General.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Ship radio equipment-General. 80.1085 Section... Requirements for Ship Stations § 80.1085 Ship radio equipment—General. This section contains the general equipment requirements for all ships subject to this subpart. (a) Ships must be provided with: (1) A VHF...

47 CFR 80.1189 - Portable ship earth stations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Portable ship earth stations. 80.1189 Section....1189 Portable ship earth stations. (a) Portable ship earth stations are authorized to operate on board more than one ship. Portable ship earth stations are also authorized to be operated on board fixed...

47 CFR 80.1189 - Portable ship earth stations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Portable ship earth stations. 80.1189 Section....1189 Portable ship earth stations. (a) Portable ship earth stations are authorized to operate on board more than one ship. Portable ship earth stations are also authorized to be operated on board fixed...

47 CFR 80.1189 - Portable ship earth stations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Portable ship earth stations. 80.1189 Section....1189 Portable ship earth stations. (a) Portable ship earth stations are authorized to operate on board more than one ship. Portable ship earth stations are also authorized to be operated on board fixed...

47 CFR 80.1189 - Portable ship earth stations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 5 2013-10-01 2013-10-01 false Portable ship earth stations. 80.1189 Section....1189 Portable ship earth stations. (a) Portable ship earth stations are authorized to operate on board more than one ship. Portable ship earth stations are also authorized to be operated on board fixed...

47 CFR 80.1189 - Portable ship earth stations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Portable ship earth stations. 80.1189 Section....1189 Portable ship earth stations. (a) Portable ship earth stations are authorized to operate on board more than one ship. Portable ship earth stations are also authorized to be operated on board fixed...

46 CFR 173.052 - Civilian nautical school ships.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Civilian nautical school ships. 173.052 Section 173.052 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SUBDIVISION AND STABILITY SPECIAL RULES PERTAINING TO VESSEL USE School Ships § 173.052 Civilian nautical school ships. Each civilian nautical school...

46 CFR 173.052 - Civilian nautical school ships.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Civilian nautical school ships. 173.052 Section 173.052 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SUBDIVISION AND STABILITY SPECIAL RULES PERTAINING TO VESSEL USE School Ships § 173.052 Civilian nautical school ships. Each civilian nautical school...

46 CFR 173.051 - Public nautical school ships.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Public nautical school ships. 173.051 Section 173.051 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SUBDIVISION AND STABILITY SPECIAL RULES PERTAINING TO VESSEL USE School Ships § 173.051 Public nautical school ships. Each public nautical school...

46 CFR 173.051 - Public nautical school ships.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Public nautical school ships. 173.051 Section 173.051 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SUBDIVISION AND STABILITY SPECIAL RULES PERTAINING TO VESSEL USE School Ships § 173.051 Public nautical school ships. Each public nautical school...

Emissions from Ships with respect to Their Effects on Clouds.

NASA Astrophysics Data System (ADS)

Hobbs, Peter V.; Garrett, Timothy J.; Ferek, Ronald J.; Strader, Scott R.; Hegg, Dean A.; Frick, Glendon M.; Hoppel, William A.; Gasparovic, Richard F.; Russell, Lynn M.; Johnson, Douglas W.; O'Dowd, Colin; Durkee, Philip A.; Nielsen, Kurt E.; Innis, George

2000-08-01

Emissions of particles, gases, heat, and water vapor from ships are discussed with respect to their potential for changing the microstructure of marine stratiform clouds and producing the phenomenon known as `ship tracks.' Airborne measurements are used to derive emission factors of SO2 and NO from diesel-powered and steam turbine-powered ships, burning low-grade marine fuel oil (MFO); they were 15-89 and 2-25 g kg1 of fuel burned, respectively. By contrast a steam turbine-powered ship burning high-grade navy distillate fuel had an SO2 emission factor of 6 g kg1.Various types of ships, burning both MFO and navy distillate fuel, emitted from 4 × 1015 to 2 × 1016 total particles per kilogram of fuel burned (4 × 1015-1.5 × 1016 particles per second). However, diesel-powered ships burning MFO emitted particles with a larger mode radius (0.03-0.05 m) and larger maximum sizes than those powered by steam turbines burning navy distillate fuel (mode radius 0.02 m). Consequently, if the particles have similar chemical compositions, those emitted by diesel ships burning MFO will serve as cloud condensation nuclei (CCN) at lower supersaturations (and will therefore be more likely to produce ship tracks) than the particles emitted by steam turbine ships burning distillate fuel. Since steam turbine-powered ships fueled by MFO emit particles with a mode radius similar to that of diesel-powered ships fueled by MFO, it appears that, for given ambient conditions, the type of fuel burned by a ship is more important than the type of ship engine in determining whether or not a ship will produce a ship track. However, more measurements are needed to test this hypothesis.The particles emitted from ships appear to be primarily organics, possibly combined with sulfuric acid produced by gas-to-particle conversion of SO2. Comparison of model results with measurements in ship tracks suggests that the particles from ships contain only about 10% water-soluble materials. Measurements of the total particles entering marine stratiform clouds from diesel-powered ships fueled by MFO, and increases in droplet concentrations produced by these particles, show that only about 12% of the particles serve as CCN.The fluxes of heat and water vapor from ships are estimated to be 2-22 MW and 0.5-1.5 kg s1, respectively. These emissions rarely produced measurable temperature perturbations, and never produced detectable perturbations in water vapor, in the plumes from ships. Nuclear-powered ships, which emit heat but negligible particles, do not produce ship tracks. Therefore, it is concluded that heat and water vapor emissions do not play a significant role in ship track formation and that particle emissions, particularly from those burning low-grade fuel oil, are responsible for ship track formation. Subsequent papers in this special issue discuss and test these hypotheses.

47 CFR 80.79 - Inspection of ship station by a foreign Government.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Inspection of ship station by a foreign... Requirements-Ship Stations § 80.79 Inspection of ship station by a foreign Government. The Governments or appropriate administrations of countries which a ship visits may require the license of the ship station or...

46 CFR 111.10-7 - Dead ship.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Dead ship. 111.10-7 Section 111.10-7 Shipping COAST... REQUIREMENTS Power Supply § 111.10-7 Dead ship. (a) The generating plant of each self-propelled vessel must provide the electrical services necessary to start the main propulsion plant from a dead ship condition...

46 CFR 111.10-7 - Dead ship.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Dead ship. 111.10-7 Section 111.10-7 Shipping COAST... REQUIREMENTS Power Supply § 111.10-7 Dead ship. (a) The generating plant of each self-propelled vessel must provide the electrical services necessary to start the main propulsion plant from a dead ship condition...

46 CFR 111.10-7 - Dead ship.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Dead ship. 111.10-7 Section 111.10-7 Shipping COAST... REQUIREMENTS Power Supply § 111.10-7 Dead ship. (a) The generating plant of each self-propelled vessel must provide the electrical services necessary to start the main propulsion plant from a dead ship condition...

46 CFR 111.10-7 - Dead ship.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Dead ship. 111.10-7 Section 111.10-7 Shipping COAST... REQUIREMENTS Power Supply § 111.10-7 Dead ship. (a) The generating plant of each self-propelled vessel must provide the electrical services necessary to start the main propulsion plant from a dead ship condition...

46 CFR 111.10-7 - Dead ship.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Dead ship. 111.10-7 Section 111.10-7 Shipping COAST... REQUIREMENTS Power Supply § 111.10-7 Dead ship. (a) The generating plant of each self-propelled vessel must provide the electrical services necessary to start the main propulsion plant from a dead ship condition...

Identification of SHIP-1 and SHIP-2 homologs in channel catfish, Ictalurus punctatus

USDA-ARS?s Scientific Manuscript database

Src homology domain 2 (SH2) domain-containing inositol 5’-phosphatases (SHIP) proteins have diverse roles in signal transduction. SHIP-1 and SHIP-2 homologs were identified in channel catfish, Ictalurus punctatus, based on sequence homology to murine and human SHIP sequences. Full-length cDNAs for ...

46 CFR 147.15 - Hazardous ships' stores permitted on board vessels.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Hazardous ships' stores permitted on board vessels. 147... HAZARDOUS SHIPS' STORES General Provisions § 147.15 Hazardous ships' stores permitted on board vessels. Unless prohibited under subpart B of this part, any hazardous material may be on board a vessel as ships...

46 CFR Sec. 5 - Measures to protect ship's payrolls.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Measures to protect ship's payrolls. Sec. 5 Section 5... SHIP'S PERSONNEL Sec. 5 Measures to protect ship's payrolls. (a) General Agents are not required to... paying off the crew should be either the Master, or purser, or some other member of the ship's personnel...

46 CFR 2.75-60 - Hazardous ships' stores.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Hazardous ships' stores. 2.75-60 Section 2.75-60 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY PROCEDURES APPLICABLE TO THE PUBLIC VESSEL... Personnel § 2.75-60 Hazardous ships' stores. Hazardous ships' stores, as defined in § 147.3 of this chapter...

46 CFR Sec. 5 - Measures to protect ship's payrolls.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 8 2011-10-01 2011-10-01 false Measures to protect ship's payrolls. Sec. 5 Section 5... SHIP'S PERSONNEL Sec. 5 Measures to protect ship's payrolls. (a) General Agents are not required to... paying off the crew should be either the Master, or purser, or some other member of the ship's personnel...

46 CFR 2.75-60 - Hazardous ships' stores.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 1 2011-10-01 2011-10-01 false Hazardous ships' stores. 2.75-60 Section 2.75-60 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY PROCEDURES APPLICABLE TO THE PUBLIC VESSEL... Personnel § 2.75-60 Hazardous ships' stores. Hazardous ships' stores, as defined in § 147.3 of this chapter...

46 CFR 167.15-20 - Inspections of nautical school ships.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Inspections of nautical school ships. 167.15-20 Section 167.15-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS PUBLIC NAUTICAL SCHOOL SHIPS Inspections § 167.15-20 Inspections of nautical school ships. (a) At each annual...

46 CFR 173.052 - Civilian nautical school ships.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Civilian nautical school ships. 173.052 Section 173.052... PERTAINING TO VESSEL USE School Ships § 173.052 Civilian nautical school ships. Each civilian nautical school ship must comply with part 171 of this subchapter as though it were a passenger vessel. In addition to...

46 CFR 173.052 - Civilian nautical school ships.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Civilian nautical school ships. 173.052 Section 173.052... PERTAINING TO VESSEL USE School Ships § 173.052 Civilian nautical school ships. Each civilian nautical school ship must comply with part 171 of this subchapter as though it were a passenger vessel. In addition to...

46 CFR 147.15 - Hazardous ships' stores permitted on board vessels.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Hazardous ships' stores permitted on board vessels. 147... HAZARDOUS SHIPS' STORES General Provisions § 147.15 Hazardous ships' stores permitted on board vessels. Unless prohibited under subpart B of this part, any hazardous material may be on board a vessel as ships...

46 CFR 147.15 - Hazardous ships' stores permitted on board vessels.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Hazardous ships' stores permitted on board vessels. 147... HAZARDOUS SHIPS' STORES General Provisions § 147.15 Hazardous ships' stores permitted on board vessels. Unless prohibited under subpart B of this part, any hazardous material may be on board a vessel as ships...

46 CFR 2.75-60 - Hazardous ships' stores.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 1 2012-10-01 2012-10-01 false Hazardous ships' stores. 2.75-60 Section 2.75-60 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY PROCEDURES APPLICABLE TO THE PUBLIC VESSEL... Personnel § 2.75-60 Hazardous ships' stores. Hazardous ships' stores, as defined in § 147.3 of this chapter...

46 CFR 173.051 - Public nautical school ships.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Public nautical school ships. 173.051 Section 173.051... PERTAINING TO VESSEL USE School Ships § 173.051 Public nautical school ships. Each public nautical school ship must comply with— (a) Section 171.070(a) of this subchapter as a passenger vessel carrying 400 or...

46 CFR Sec. 5 - Measures to protect ship's payrolls.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 8 2014-10-01 2014-10-01 false Measures to protect ship's payrolls. Sec. 5 Section 5... SHIP'S PERSONNEL Sec. 5 Measures to protect ship's payrolls. (a) General Agents are not required to... paying off the crew should be either the Master, or purser, or some other member of the ship's personnel...

46 CFR 173.051 - Public nautical school ships.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Public nautical school ships. 173.051 Section 173.051... PERTAINING TO VESSEL USE School Ships § 173.051 Public nautical school ships. Each public nautical school ship must comply with— (a) Section 171.070(a) of this subchapter as a passenger vessel carrying 400 or...

46 CFR Sec. 5 - Measures to protect ship's payrolls.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 8 2013-10-01 2013-10-01 false Measures to protect ship's payrolls. Sec. 5 Section 5... SHIP'S PERSONNEL Sec. 5 Measures to protect ship's payrolls. (a) General Agents are not required to... paying off the crew should be either the Master, or purser, or some other member of the ship's personnel...

46 CFR 167.15-20 - Inspections of nautical school ships.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Inspections of nautical school ships. 167.15-20 Section 167.15-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS PUBLIC NAUTICAL SCHOOL SHIPS Inspections § 167.15-20 Inspections of nautical school ships. (a) At each annual...

46 CFR 173.051 - Public nautical school ships.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Public nautical school ships. 173.051 Section 173.051... PERTAINING TO VESSEL USE School Ships § 173.051 Public nautical school ships. Each public nautical school ship must comply with— (a) Section 171.070(a) of this subchapter as a passenger vessel carrying 400 or...

46 CFR 173.052 - Civilian nautical school ships.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Civilian nautical school ships. 173.052 Section 173.052... PERTAINING TO VESSEL USE School Ships § 173.052 Civilian nautical school ships. Each civilian nautical school ship must comply with part 171 of this subchapter as though it were a passenger vessel. In addition to...

46 CFR 2.75-60 - Hazardous ships' stores.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 1 2013-10-01 2013-10-01 false Hazardous ships' stores. 2.75-60 Section 2.75-60 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY PROCEDURES APPLICABLE TO THE PUBLIC VESSEL... Personnel § 2.75-60 Hazardous ships' stores. Hazardous ships' stores, as defined in § 147.3 of this chapter...

46 CFR 167.15-20 - Inspections of nautical school ships.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Inspections of nautical school ships. 167.15-20 Section 167.15-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS PUBLIC NAUTICAL SCHOOL SHIPS Inspections § 167.15-20 Inspections of nautical school ships. (a) At each annual...

46 CFR 147.15 - Hazardous ships' stores permitted on board vessels.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Hazardous ships' stores permitted on board vessels. 147... HAZARDOUS SHIPS' STORES General Provisions § 147.15 Hazardous ships' stores permitted on board vessels. Unless prohibited under subpart B of this part, any hazardous material may be on board a vessel as ships...

46 CFR 151.45-7 - Shipping papers.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Shipping papers. 151.45-7 Section 151.45-7 Shipping... BULK LIQUID HAZARDOUS MATERIAL CARGOES Operations § 151.45-7 Shipping papers. Each barge carrying... towing vessel shall either have a copy of the shipping papers for each barge in his tow or he shall make...

46 CFR 148.02-1 - Shipping papers.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Shipping papers. 148.02-1 Section 148.02-1 Shipping... MATERIALS IN BULK Vessel Requirements § 148.02-1 Shipping papers. (a) Carriers may not accept for..., unless the hazardous materials offered for such shipment is accompanied by a shipping paper on which the...

46 CFR 151.45-7 - Shipping papers.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Shipping papers. 151.45-7 Section 151.45-7 Shipping... BULK LIQUID HAZARDOUS MATERIAL CARGOES Operations § 151.45-7 Shipping papers. Each barge carrying... towing vessel shall either have a copy of the shipping papers for each barge in his tow or he shall make...

46 CFR 2.75-60 - Hazardous ships' stores.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 1 2014-10-01 2014-10-01 false Hazardous ships' stores. 2.75-60 Section 2.75-60 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY PROCEDURES APPLICABLE TO THE PUBLIC VESSEL... Personnel § 2.75-60 Hazardous ships' stores. Hazardous ships' stores, as defined in § 147.3 of this chapter...

46 CFR 167.15-20 - Inspections of nautical school ships.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Inspections of nautical school ships. 167.15-20 Section 167.15-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS PUBLIC NAUTICAL SCHOOL SHIPS Inspections § 167.15-20 Inspections of nautical school ships. (a) At each annual...

46 CFR 167.15-20 - Inspections of nautical school ships.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Inspections of nautical school ships. 167.15-20 Section 167.15-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS PUBLIC NAUTICAL SCHOOL SHIPS Inspections § 167.15-20 Inspections of nautical school ships. (a) At each annual...

Analysis of radiation exposure for naval units of Operation Crossroads. Volume 3. (Appendix B) support ships. Technical report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Weitz, R.; Thomas, C.; Klemm, J.

1982-03-03

External radiation doses are reconstructed for crews of support and target ships of Joint Task Force One at Operation CROSSROADS, 1946. Volume I describes the reconstruction methodology, which consists of modeling the radiation environment, to include the radioactivity of lagoon water, target ships, and support ship contamination; retracing ship paths through this environment; and calculating the doses to shipboard personnel. The USS RECLAIMER, a support ship, is selected as a representative ship to demonstrate this methodology. Doses for all other ships are summarized. Volume II (Appendix A) details the results for target ship personnel. Volume III (Appendix B) details themore » results for support ship personnel. Calculated doses for more than 36,000 personnel aboard support ships while at Bikini range from zero to 1.7 rem. Of those approximately 34,000 are less than 0.5 rem. From the models provided, doses due to target ship reboarding and doses accrued after departure from Bikini can be calculated, based on the individual circumstances of exposure.« less

Analysis of radiation exposure for naval units of Operation Crossroads. Volume 2. (Appendix A) target ships. Technical report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Weitz, R.; Thomas, C.; Klemm, J.

1982-03-03

External radiation doses are reconstructed for crews of support and target ships of Joint Task Force One at Operation CROSSROADS, 1946. Volume I describes the reconstruction methodology, which consists of modeling the radiation environment, to include the radioactivity of lagoon water, target ships, and support ship contamination; retracing ship paths through this environment; and calculating the doses to shipboard personnel. The USS RECLAIMER, a support ship, is selected as a representative ship to demonstrate this methodology. Doses for all other ships are summarized. Volume II (Appendix A) details the results for target ship personnel. Volume III (Appendix B) details themore » results for support ship personnel. Calculated doses for more than 36,000 personnel aboard support ships while at Bikini range from zero to 1.7 rem. Of those, approximately 34,000 are less than 0.5 rem. From the models provided, doses due to target ship reboarding and doses accrued after departure from Bikini can be calculated, based on the individual circumstances of exposure.« less

Infections on Cruise Ships.

PubMed

Kak, Vivek

2015-08-01

The modern cruise ship is a small city on the seas, with populations as large as 5,000 seen on large ships. The growth of the cruise ship industry has continued in the twenty-first century, and it was estimated that nearly 21.3 million passengers traveled on cruise ships in 2013, with the majority of these sailing from North America. The presence of large numbers of individuals in close proximity to each other facilitates transmission of infectious diseases, often through person-to-person spread or via contaminated food or water. An infectious agent introduced into the environment of a cruise ship has the potential to be distributed widely across the ship and to cause significant morbidity. The median cruise ship passenger is over 45 years old and often has chronic medical problems, so it is important that, to have a safe cruise ship experience, any potential for the introduction of an infecting agent as well as its transmission be minimized. The majority of cruise ship infections involve respiratory and gastrointestinal infections. This article discusses infectious outbreaks on cruise ships and suggests preventative measures for passengers who plan to travel on cruise ships.

Gaussian mixture models-based ship target recognition algorithm in remote sensing infrared images

NASA Astrophysics Data System (ADS)

Yao, Shoukui; Qin, Xiaojuan

2018-02-01

Since the resolution of remote sensing infrared images is low, the features of ship targets become unstable. The issue of how to recognize ships with fuzzy features is an open problem. In this paper, we propose a novel ship target recognition algorithm based on Gaussian mixture models (GMMs). In the proposed algorithm, there are mainly two steps. At the first step, the Hu moments of these ship target images are calculated, and the GMMs are trained on the moment features of ships. At the second step, the moment feature of each ship image is assigned to the trained GMMs for recognition. Because of the scale, rotation, translation invariance property of Hu moments and the power feature-space description ability of GMMs, the GMMs-based ship target recognition algorithm can recognize ship reliably. Experimental results of a large simulating image set show that our approach is effective in distinguishing different ship types, and obtains a satisfactory ship recognition performance.

Relationship between container ship underwater noise levels and ship design, operational and oceanographic conditions

PubMed Central

McKenna, Megan F.; Wiggins, Sean M.; Hildebrand, John A.

2013-01-01

Low-frequency ocean ambient noise is dominated by noise from commercial ships, yet understanding how individual ships contribute deserves further investigation. This study develops and evaluates statistical models of container ship noise in relation to design characteristics, operational conditions, and oceanographic settings. Five-hundred ship passages and nineteen covariates were used to build generalized additive models. Opportunistic acoustic measurements of ships transiting offshore California were collected using seafloor acoustic recorders. A 5–10 dB range in broadband source level was found for ships depending on the transit conditions. For a ship recorded multiple times traveling at different speeds, cumulative noise was lowest at 8 knots, 65% reduction in operational speed. Models with highest predictive power, in order of selection, included ship speed, size, and time of year. Uncertainty in source depth and propagation affected model fit. These results provide insight on the conditions that produce higher levels of underwater noise from container ships.

Influence of Ship Emissions on Urban Air Quality: A Comprehensive Study Using Highly Time-Resolved Online Measurements and Numerical Simulation in Shanghai.

PubMed

Liu, Zhanmin; Lu, Xiaohui; Feng, Junlan; Fan, Qianzhu; Zhang, Yan; Yang, Xin

2017-01-03

Shanghai has become an international shipping center in the world. In this study, the multiyear measurements and the high resolution air quality model with hourly ship emission inventory were combined to determine the influence of ship emissions on urban Shanghai. The aerosol time-of-flight mass spectrometer (ATOFMS) measurements were carried out at an urban site from April 2009 to January 2013. During the entire sampling time, most of the half-hourly averaged number fractions of primary ship emitted particles varied between 1.0-10.0%. However, the number fraction could reach up to 50% during the ship plume cases. Ship-plume-influenced periods usually occurred in spring and summer. The simulation of Weather Research and Forecasting/Community Multiscale Air Quality model (WRF/CMAQ) with hourly ship emission inventory provided the highly time-resolved concentrations of ship-related air pollutants during a ship plume case. It showed ships could contribute 20-30% (2-7 μg/m 3 ) of the total PM 2.5 within tens of kilometers of coastal and riverside Shanghai during ship-plume-influenced periods. Our results showed that ship emissions have substantial contribution to the air pollution in urban Shanghai. The control measures of ship emission should be taken considering its negative environment and human health effects.

46 CFR 153.12 - IMO Certificates for United States Ships.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false IMO Certificates for United States Ships. 153.12 Section 153.12 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS General § 153.12 IMO Certificates for United States Ships....

46 CFR 147.15 - Hazardous ships' stores permitted on board vessels.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Hazardous ships' stores permitted on board vessels. 147.15 Section 147.15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) DANGEROUS CARGOES HAZARDOUS SHIPS' STORES General Provisions § 147.15 Hazardous ships' stores permitted on board vessels. Unless prohibited under subpart B of this...

27 CFR 26.114 - Permit to ship required.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Permit to ship required... Liquors and Articles in Puerto Rico Permit to Ship Liquors and Articles § 26.114 Permit to ship required... paid or deferred as prescribed in this subpart, may be shipped to the United States, a permit to ship...

27 CFR 26.114 - Permit to ship required.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false Permit to ship required... Liquors and Articles in Puerto Rico Permit to Ship Liquors and Articles § 26.114 Permit to ship required... paid or deferred as prescribed in this subpart, may be shipped to the United States, a permit to ship...

27 CFR 26.114 - Permit to ship required.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false Permit to ship required... Liquors and Articles in Puerto Rico Permit to Ship Liquors and Articles § 26.114 Permit to ship required... paid or deferred as prescribed in this subpart, may be shipped to the United States, a permit to ship...

27 CFR 26.114 - Permit to ship required.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false Permit to ship required... Liquors and Articles in Puerto Rico Permit to Ship Liquors and Articles § 26.114 Permit to ship required... paid or deferred as prescribed in this subpart, may be shipped to the United States, a permit to ship...

27 CFR 26.114 - Permit to ship required.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false Permit to ship required... Liquors and Articles in Puerto Rico Permit to Ship Liquors and Articles § 26.114 Permit to ship required... paid or deferred as prescribed in this subpart, may be shipped to the United States, a permit to ship...

47 CFR 80.1123 - Watch requirements for ship stations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... such information is broadcast for the area in which the ship is navigating. (c) Every ship while at sea... at sea, all ships must maintain a continuous watch: (1) On VHF DSC channel 70, if the ship is fitted... earth station in accordance with § 80.1091(a)(1). (b) While at sea, all ships must maintain radio...

47 CFR 80.1123 - Watch requirements for ship stations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... such information is broadcast for the area in which the ship is navigating. (c) Every ship while at sea... at sea, all ships must maintain a continuous watch: (1) On VHF DSC channel 70, if the ship is fitted... earth station in accordance with § 80.1091(a)(1). (b) While at sea, all ships must maintain radio...

47 CFR 80.1123 - Watch requirements for ship stations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... such information is broadcast for the area in which the ship is navigating. (c) Every ship while at sea... at sea, all ships must maintain a continuous watch: (1) On VHF DSC channel 70, if the ship is fitted... earth station in accordance with § 80.1091(a)(1). (b) While at sea, all ships must maintain radio...

Impact of future Arctic shipping on high-latitude black carbon deposition (Invited)

NASA Astrophysics Data System (ADS)

Corbett, J. J.; Browse, J.; Carslaw, K. S.; Schmidt, A.

2013-12-01

The retreat of Arctic sea-ice has led to renewed calls to exploit Arctic shipping routes. The diversion of ship traffic through the Arctic will shorten shipping routes and possibly reduce global shipping emissions. However, deposition of black carbon (BC) aerosol emitted by additional Arctic ships could cause a reduction in the albedo of snow and ice, accelerating snow-melt and sea-ice loss. We use recently compiled Arctic shipping emission inventories for 2004 and 2050 together with a global aerosol microphysics model GLOMAP coupled to the chemical transport model TOMCAT to quantify the contribution of future Arctic shipping to high-latitude BC deposition. Emission rates of SOx (SO2 and SO4) and particulate matter (PM) were estimated for 2050 under both business-as-usual and high-growth scenarios. BC particles are assumed to be water-insoluble at emission but can become active in cloud drop formation through soluble material accumulation. After BC particles become cloud-active they are more efficiently wet scavenged, which accounts for 80% of modeled BC deposition. Current-day Arctic shipping contributes 0.3% to the BC mass deposited north of 60N (250 Gg). About 50% of modelled BC deposition is on open ocean, suggesting that current Arctic ship traffic may not significantly contribute to BC deposition on central Arctic sea ice. However, 6 - 8% of deposited BC on the west coast of Greenland originates from local ship traffic. Moreover, in-Arctic shipping contributes some 32% to high-latitude ship-sourced deposition despite accounting for less than 1.0% of global shipping emissions. This suggests that control of in-Arctic shipping BC emissions could yield greater decrease in high-latitude BC deposition than a similar control strategy applied only to the extra-Arctic shipping industry. Arctic shipping in 2050 will contribute less than 1% to the total BC deposition north of 60N due to the much greater relative contribution of BC transported from non-shipping sources at lower-latitudes (with a maximum of about 5%, considering upper bound estimates for transport). In the BAU and HiG scenarios, the total BC deposition averaged north of 60N from Arctic shipping remains small, increasing to only 0.4% and 0.7%, respectively. Several mitigation strategies confirmed that extra-Arctic sources other than shipping contribute significantly more to BC deposition than Arctic shipping, and that regulation solely aimed at the Arctic shipping industry is an insufficient control on high-latitude BC deposition. An exception is the impact of local shipping near the vulnerable Greenland ice-sheet. Over Greenland the deposited BC mass attributable to high-growth shipping emissions in 2050 is significantly higher (10-15%) than over Arctic sea-ice. The increase in local BC deposition over Greenland can be mitigated by a 10% decrease in North American BC emissions, but additional controls over distant stationary sources should be considered alongside international agreements controlling shipping emissions to achieve desired Arctic BC deposition reductions.

A cellular automaton model for ship traffic flow in waterways

NASA Astrophysics Data System (ADS)

Qi, Le; Zheng, Zhongyi; Gang, Longhui

2017-04-01

With the development of marine traffic, waterways become congested and more complicated traffic phenomena in ship traffic flow are observed. It is important and necessary to build a ship traffic flow model based on cellular automata (CAs) to study the phenomena and improve marine transportation efficiency and safety. Spatial discretization rules for waterways and update rules for ship movement are two important issues that are very different from vehicle traffic. To solve these issues, a CA model for ship traffic flow, called a spatial-logical mapping (SLM) model, is presented. In this model, the spatial discretization rules are improved by adding a mapping rule. And the dynamic ship domain model is considered in the update rules to describe ships' interaction more exactly. Take the ship traffic flow in the Singapore Strait for example, some simulations were carried out and compared. The simulations show that the SLM model could avoid ship pseudo lane-change efficiently, which is caused by traditional spatial discretization rules. The ship velocity change in the SLM model is consistent with the measured data. At finally, from the fundamental diagram, the relationship between traffic ability and the lengths of ships is explored. The number of ships in the waterway declines when the proportion of large ships increases.

Are nuclear ships environmentally safer than conventionally powered ships

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bone, C.A.; Molgaard, C.A.; Helmkamp, J.C.

1988-03-01

An epidemiologic analysis was conducted to determine if risk of hospitalization varied by age, ship type, or occupation between nuclear and conventional powered ship crews in the U.S. Navy. Study cohorts consisted of all male enlisted personnel who served exclusively aboard conventional or nuclear powered aircraft carriers and cruisers during the years 1975-1979; cases were those men hospitalized during this period (N = 48,242). Conventional ship personnel showed significantly elevated rates of injury and disease when compared to nuclear ship personnel. The largest relative risks by age occurred for conventional ship crewmen less than 30 years old. Seaman, logistics (supply),more » and healthcare personnel serving aboard conventional ships comprised the occupational groups exhibiting the highest hospitalization rate differentials. The results strongly suggest that nuclear ships provide a healthier, safer working and living environment than conventional ships.« less

The SH2-containing inositol polyphosphate 5-phosphatase, SHIP-2, binds filamin and regulates submembraneous actin

PubMed Central

Dyson, Jennifer M.; O'Malley, Cindy J.; Becanovic, Jelena; Munday, Adam D.; Berndt, Michael C.; Coghill, Imogen D.; Nandurkar, Harshal H.; Ooms, Lisa M.; Mitchell, Christina A.

2001-01-01

SHIP-2 is a phosphoinositidylinositol 3,4,5 trisphosphate (PtdIns[3,4,5]P3) 5-phosphatase that contains an NH2-terminal SH2 domain, a central 5-phosphatase domain, and a COOH-terminal proline-rich domain. SHIP-2 negatively regulates insulin signaling. In unstimulated cells, SHIP-2 localized in a perinuclear cytosolic distribution and at the leading edge of the cell. Endogenous and recombinant SHIP-2 localized to membrane ruffles, which were mediated by the COOH-terminal proline–rich domain. To identify proteins that bind to the SHIP-2 proline–rich domain, yeast two-hybrid screening was performed, which isolated actin-binding protein filamin C. In addition, both filamin A and B specifically interacted with SHIP-2 in this assay. SHIP-2 coimmunoprecipitated with filamin from COS-7 cells, and association between these species did not change after epidermal growth factor stimulation. SHIP-2 colocalized with filamin at Z-lines and the sarcolemma in striated muscle sections and at membrane ruffles in COS-7 cells, although the membrane ruffling response was reduced in cells overexpressing SHIP-2. SHIP-2 membrane ruffle localization was dependent on filamin binding, as SHIP-2 was expressed exclusively in the cytosol of filamin-deficient cells. Recombinant SHIP-2 regulated PtdIns(3,4,5)P3 levels and submembraneous actin at membrane ruffles after growth factor stimulation, dependent on SHIP-2 catalytic activity. Collectively these studies demonstrate that filamin-dependent SHIP-2 localization critically regulates phosphatidylinositol 3 kinase signaling to the actin cytoskeleton. PMID:11739414

48 CFR 1336.270 - Special requirements for ship construction

Code of Federal Regulations, 2011 CFR

2011-10-01

... ship construction 1336.270 Section 1336.270 Federal Acquisition Regulations System DEPARTMENT OF... Contracting for Construction 1336.270 Special requirements for ship construction See 48 CFR 1371 for special requirements for acquisition involving ship construction and ship repair. ...

48 CFR 1336.270 - Special requirements for ship construction

Code of Federal Regulations, 2014 CFR

2014-10-01

... ship construction 1336.270 Section 1336.270 Federal Acquisition Regulations System DEPARTMENT OF... Contracting for Construction 1336.270 Special requirements for ship construction See 48 CFR 1371 for special requirements for acquisition involving ship construction and ship repair. ...

48 CFR 1336.270 - Special requirements for ship construction

Code of Federal Regulations, 2012 CFR

2012-10-01

... ship construction 1336.270 Section 1336.270 Federal Acquisition Regulations System DEPARTMENT OF... Contracting for Construction 1336.270 Special requirements for ship construction See 48 CFR 1371 for special requirements for acquisition involving ship construction and ship repair. ...

48 CFR 1336.270 - Special requirements for ship construction

Code of Federal Regulations, 2013 CFR

2013-10-01

... ship construction 1336.270 Section 1336.270 Federal Acquisition Regulations System DEPARTMENT OF... Contracting for Construction 1336.270 Special requirements for ship construction See 48 CFR 1371 for special requirements for acquisition involving ship construction and ship repair. ...

46 CFR 167.01-8 - Inspection of school ships using gross tonnage criterion.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Inspection of school ships using gross tonnage criterion. 167.01-8 Section 167.01-8 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS PUBLIC NAUTICAL SCHOOL SHIPS General Provisions § 167.01-8 Inspection of school ships using gross tonnage criterion. (a) One of the...

26 CFR 1.883-1 - Exclusion of income from the international operation of ships or aircraft.

Code of Federal Regulations, 2010 CFR

2010-04-01

... during the time it is an owner or lessee of one or more entire ships or aircraft and uses such ships or... the case of a ship, the leasing out of the ship under a time or voyage charter (full charter), space...; (v) Ship or aircraft brokering; (vi) Freight forwarding; (vii) The activities of travel agents and...

Present-day and future global bottom-up ship emission inventories including polar routes.

PubMed

Paxian, Andreas; Eyring, Veronika; Beer, Winfried; Sausen, Robert; Wright, Claire

2010-02-15

We present a global bottom-up ship emission algorithm that calculates fuel consumption, emissions, and vessel traffic densities for present-day (2006) and two future scenarios (2050) considering the opening of Arctic polar routes due to projected sea ice decline. Ship movements and actual ship engine power per individual ship from Lloyd's Marine Intelligence Unit (LMIU) ship statistics for six months in 2006 and further mean engine data from literature serve as input. The developed SeaKLIM algorithm automatically finds the most probable shipping route for each combination of start and destination port of a certain ship movement by calculating the shortest path on a predefined model grid while considering land masses, sea ice, shipping canal sizes, and climatological mean wave heights. The resulting present-day ship activity agrees well with observations. The global fuel consumption of 221 Mt in 2006 lies in the range of previously published inventories when undercounting of ship numbers in the LMIU movement database (40,055 vessels) is considered. Extrapolated to 2007 and ship numbers per ship type of the recent International Maritime Organization (IMO) estimate (100,214 vessels), a fuel consumption of 349 Mt is calculated which is in good agreement with the IMO total of 333 Mt. The future scenarios show Arctic polar routes with regional fuel consumption on the Northeast and Northwest Passage increasing by factors of up to 9 and 13 until 2050, respectively.

Standard Technical Manual Identification Numbering System (TMINS). M0000-00-IDX-000 TMINS

DTIC Science & Technology

1980-05-14

a part, assign’it to a numbered category. For exam- ple, a ship propulsion plant should be assigned to category 9 ( Ships /Craft) while an...SSN At t.u k Silcma t i ne rio l Iea r p we red 9 - AGS Survey i ig Ship Mine Warfare Ships 9-Afl0 Holspital Ship 9-AKI, Li Cargo Ship 9- AKR Vehicle...which it is a part, such as a ship propulsion plant, select a numeric category and series from Table 2-2

THE EFFECT OF FORWARD SPEED ON DAMPING FOR A VARIETY OF SHIP TYPES AS CALCULATED BY THIN SHIP THEORY,

DTIC Science & Technology

Since the damping coefficients play a predominant role in the motion response of ships in pitch and heave at resonant frequencies in a seaway, use...was made of two computer programs recently developed at M. I. T. to calculate, by thin ship theory, the effect of ship speed on the damping coefficients...in pitch and heave for four diverse types of ship hulls--cargo ship, tanker, destroyer, and trawler. Results indicate that, for all four hull types

The influence of ship's stability on safety of navigation

NASA Astrophysics Data System (ADS)

Hanzu-Pazara, R.; Duse; Varsami, C.; Andrei, C.; Dumitrache, R.

2016-08-01

Ship's stability is one of the most important and complex concept about safety of ship and safety of navigation and it is governed by maritime law as well as maritime codes. The paper presents the importance of ship's intact stability as part of the general concept of ship's seaworthiness. There is always a correlation between ship’ stability and safety of ship and safety of navigation. Loss of ship's stability is presented as a threat to safety of navigation. We are going to present the causes that lead to ship stability failure and their impact on safety of navigation. A study of various ship stability casualties in heavy weather conditions are going to be presented, the causes are going to be analyzed and the possible ways of stability failures are assessed. Vessel's intact stability is a fundamental component of seaworthiness so it is in the interest of all owners/operators to learn about this topic and ensure that their vessel possesses a satisfactory level of stability in order to ensure its safety as well as that of the people on board the ship. Understanding ship's stability, trim, stress, and the basics of ship's construction is a key to keeping a ship seaworthy. The findings of this study can be beneficial to the maritime safety administrations to adopt decision-making on maritime safety management, but it is also important to carry out statistics and analysis of marine casualties to help to adopt proper safety management measures. Moreover, the study can be a useful guidance for masters and officers on board vessel in order to understand the factors that contribute to ship stability failure during the voyage not only in port during loading operations and to take preventive measures to avoid to put the ship in such a dangerous situations.

Feature long axis size and local luminance contrast determine ship target acquisition performance: strong evidence for the TOD case

NASA Astrophysics Data System (ADS)

Bijl, Piet; Toet, Alexander; Kooi, Frank L.

2016-10-01

Visual images of a civilian target ship on a sea background were produced using a CAD model. The total set consisted of 264 images and included 3 different color schemes, 2 ship viewing aspects, 5 sun illumination conditions, 2 sea reflection values, 2 ship positions with respect to the horizon and 3 values of atmospheric contrast reduction. In a perception experiment, the images were presented on a display in a long darkened corridor. Observers were asked to indicate the range at which they were able to detect the ship and classify the following 5 ship elements: accommodation, funnel, hull, mast, and hat above the bridge. This resulted in a total of 1584 Target Acquisition (TA) range estimates for two observers. Next, the ship contour, ship elements and corresponding TA ranges were analyzed applying several feature size and contrast measures. Most data coincide on a contrast versus angular size plot using (1) the long axis as characteristic ship/ship feature size and (2) local Weber contrast as characteristic ship/ship feature contrast. Finally, the data were compared with a variety of visual performance functions assumed to be representative for Target Acquisition: the TOD (Triangle Orientation Discrimination), MRC (Minimum Resolvable Contrast), CTF (Contrast Threshold Function), TTP (Targeting Task Performance) metric and circular disc detection data for the unaided eye (Blackwell). The results provide strong evidence for the TOD case: both position and slope of the TOD curve match the ship detection and classification data without any free parameter. In contrast, the MRC and CTF are too steep, the TTP and disc detection curves are too shallow and all these curves need an overall scaling factor in order to coincide with the ship and ship feature recognition data.

International Standardization in the Design of "Shore to Ship" - Power Supply Systems of Ships in Port

NASA Astrophysics Data System (ADS)

Tarnapowicz, Dariusz; German-Galkin, Sergiej

2018-03-01

The decisive source of air pollution emissions in ports is the berthed ships. This is primarily caused by the work of ship's autonomous generator sets. One way of reducing the air pollution emissions in ports is the supply of ships from electricity inland system. The main problem connected with the power connection of ships to the inland network is caused by different values of levels and frequencies of voltages in these networks (in various countries) in relation to different values of levels and frequencies of voltages present in the ship's network. It is also important that the source power can range from a few hundred kW up to several MW. In order to realize a universal „Shore to Ship" system that allows the connection of ships to the electricity inland network, the international standardization is necessary. This article presents the current recommendations, standards and regulations for the design of „Shore to Ship" systems.

Ozone production efficiency of a ship-plume: ITCT 2K2 case study.

PubMed

Kim, Hyun S; Kim, Yong H; Han, Kyung M; Kim, Jhoon; Song, Chul H

2016-01-01

Ozone production efficiency (OPE) of ship plume was first evaluated in this study, based on ship-plume photochemical/dynamic model simulations and the ship-plume composition data measured during the ITCT 2K2 (Intercontinental Transport and Chemical Transformation 2002) aircraft campaign. The averaged instantaneous OPEs (OPE(i)‾) estimated via the ship-plume photochemical/dynamic modeling for the ITCT 2K2 ship-plume ranged between 4.61 and 18.92, showing that the values vary with the extent of chemical evolution (or chemical stage) of the ship plume and the stability classes of the marine boundary layer (MBL). Together with OPE(i)‾, the equivalent OPEs (OPE(e)‾) for the entire ITCT 2K2 ship-plume were also estimated. The OPE(e)‾ values varied between 9.73 (for the stable MBL) and 12.73 (for the moderately stable MBL), which agreed well with the OPE(e)‾ of 12.85 estimated based on the ITCT 2K2 ship-plume observations. It was also found that both the model-simulated and observation-based OPE(e)‾ inside the ship-plume were 0.29-0.38 times smaller than the OPE(e)‾ calculated/measured outside the ITCT 2K2 ship-plume. Such low OPEs insides the ship plume were due to the high levels of NO and non-liner ship-plume photochemistry. Possible implications of this ship-plume OPE study in the global chemistry-transport modeling are also discussed. Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

Modeling the Impact of Arctic Shipping Pollution on Air Quality off the Coast of Northern Norway

NASA Astrophysics Data System (ADS)

Thomas, J. L.; Law, K.; Marelle, L.; Raut, J.; Jalkanen, J.; Johansson, L.; Roiger, A.; Schlager, H.; Kim, J.; Reiter, A.; Weinzierl, B.; Rose, M.; Fast, J. D.

2013-12-01

As the Arctic is undergoing rapid and potentially irreversible changes, such as the shrinking and thinning of sea-ice cover, the levels of atmospheric pollution are expected to rise dramatically due to the emergence of local pollution sources including shipping. Shipping routes through the Arctic (such as Russia's Northern Sea Route) are already used as an alternative to the traditional global transit shipping routes. In summer 2012, the ACCESS (Arctic Climate Change, Economy, and Society) aircraft campaign focused on studying pollution sources off the coast of northern Norway to quantify emissions from shipping and other anthropogenic pollution sources. To complement these measurements, a regional chemical transport model is used to study the impact of shipping pollution on gas and aerosol concentrations in the region. WRF-Chem (The Weather Research and Forecasting Model with Chemistry, which simulates gas and aerosols simultaneously with meteorology) is run with real time shipping emissions from STEAM (Ship Traffic Emission Assessment Model) for July 2012. The STEAM model calculates gas and aerosol emissions of marine traffic based on the ship type and location provided by the Automatic Identification System (AIS). Use of real time position, speed, and ship specific information allows for development of emissions with very high spatial (1x1 km) and temporal (30 min) resolution, which are used in the regional model runs. STEAM emissions have been specifically generated for shipping off the coast of Norway during the entire ACCESS campaign period. Simulated ship plumes from high-resolution model runs are compared to aircraft measurements. The regional impact of current summertime shipping is also examined. At present, relatively light ship traffic off the coast of northern Norway results in only a small impact of shipping pollution on regional atmospheric chemistry. The impact of increased future shipping on regional atmospheric chemistry is also assessed.

46 CFR 11.1103 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Definitions. 11.1103 Section 11.1103 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN REQUIREMENTS FOR OFFICER... § 11.1103 Definitions. Passenger ship in this subpart means a ship, other than a Ro-Ro passenger ship...

46 CFR 111.30-25 - Alternating-current ship's service switchboards.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Alternating-current ship's service switchboards. 111.30-25 Section 111.30-25 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Switchboards § 111.30-25 Alternating-current ship's service...

46 CFR 111.30-25 - Alternating-current ship's service switchboards.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Alternating-current ship's service switchboards. 111.30-25 Section 111.30-25 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Switchboards § 111.30-25 Alternating-current ship's service...

46 CFR 111.30-25 - Alternating-current ship's service switchboards.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Alternating-current ship's service switchboards. 111.30-25 Section 111.30-25 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Switchboards § 111.30-25 Alternating-current ship's service...

48 CFR 1371.118 - Changes-ship repair.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Changes-ship repair. 1371.118 Section 1371.118 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE DEPARTMENT SUPPLEMENTAL REGULATIONS ACQUISITIONS INVOLVING SHIP CONSTRUCTION AND SHIP REPAIR Provisions and Clauses 1371.118 Changes—ship repair. Insert clause...

32 CFR 761.12 - Ships: Group authorizations.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 5 2011-07-01 2011-07-01 false Ships: Group authorizations. 761.12 Section 761... TRUST TERRITORY OF THE PACIFIC ISLANDS Entry Authorization § 761.12 Ships: Group authorizations. Ships or other craft in the following categories, except those ships which have been denied individual...

47 CFR 80.142 - Ships using radiotelegraphy.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Ships using radiotelegraphy. 80.142 Section 80... STATIONS IN THE MARITIME SERVICES Operating Requirements and Procedures Special Procedures-Ship Stations § 80.142 Ships using radiotelegraphy. (a) Calling by narrow-band direct-printing. (1) NB-DP ship...

47 CFR 80.142 - Ships using radiotelegraphy.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Ships using radiotelegraphy. 80.142 Section 80... STATIONS IN THE MARITIME SERVICES Operating Requirements and Procedures Special Procedures-Ship Stations § 80.142 Ships using radiotelegraphy. (a) Calling by narrow-band direct-printing. (1) NB-DP ship...

32 CFR 761.12 - Ships: Group authorizations.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 5 2012-07-01 2012-07-01 false Ships: Group authorizations. 761.12 Section 761... TRUST TERRITORY OF THE PACIFIC ISLANDS Entry Authorization § 761.12 Ships: Group authorizations. Ships or other craft in the following categories, except those ships which have been denied individual...

32 CFR 761.12 - Ships: Group authorizations.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 5 2013-07-01 2013-07-01 false Ships: Group authorizations. 761.12 Section 761... TRUST TERRITORY OF THE PACIFIC ISLANDS Entry Authorization § 761.12 Ships: Group authorizations. Ships or other craft in the following categories, except those ships which have been denied individual...

47 CFR 80.142 - Ships using radiotelegraphy.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 5 2013-10-01 2013-10-01 false Ships using radiotelegraphy. 80.142 Section 80... STATIONS IN THE MARITIME SERVICES Operating Requirements and Procedures Special Procedures-Ship Stations § 80.142 Ships using radiotelegraphy. (a) Calling by narrow-band direct-printing. (1) NB-DP ship...

32 CFR 761.12 - Ships: Group authorizations.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 5 2014-07-01 2014-07-01 false Ships: Group authorizations. 761.12 Section 761... TRUST TERRITORY OF THE PACIFIC ISLANDS Entry Authorization § 761.12 Ships: Group authorizations. Ships or other craft in the following categories, except those ships which have been denied individual...

47 CFR 80.142 - Ships using radiotelegraphy.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Ships using radiotelegraphy. 80.142 Section 80... STATIONS IN THE MARITIME SERVICES Operating Requirements and Procedures Special Procedures-Ship Stations § 80.142 Ships using radiotelegraphy. (a) Calling by narrow-band direct-printing. (1) NB-DP ship...

47 CFR 80.142 - Ships using radiotelegraphy.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Ships using radiotelegraphy. 80.142 Section 80... STATIONS IN THE MARITIME SERVICES Operating Requirements and Procedures Special Procedures-Ship Stations § 80.142 Ships using radiotelegraphy. (a) Calling by narrow-band direct-printing. (1) NB-DP ship...

46 CFR 111.30-27 - Direct current ship's service switchboards.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Direct current ship's service switchboards. 111.30-27 Section 111.30-27 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Switchboards § 111.30-27 Direct current ship's service switchboards...

46 CFR 111.30-27 - Direct current ship's service switchboards.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Direct current ship's service switchboards. 111.30-27 Section 111.30-27 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Switchboards § 111.30-27 Direct current ship's service switchboards...

46 CFR 111.30-27 - Direct current ship's service switchboards.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Direct current ship's service switchboards. 111.30-27 Section 111.30-27 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Switchboards § 111.30-27 Direct current ship's service switchboards...

78 FR 51726 - Notice of Agreements Filed

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-21

... Containership Operators. Parties: A.P. Moller-Maersk A/S; China Shipping Container Lines Co., Ltd.; CMA. CGM, S... Agreement Asia--U.S. East Coast. Parties: China Shipping Container Lines Co., Ltd., China Shipping Container.... Parties: China Shipping Container Lines Co. Ltd. and China Shipping Container Lines (Hong Kong) Co., Ltd...

46 CFR 111.30-27 - Direct current ship's service switchboards.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Direct current ship's service switchboards. 111.30-27... ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Switchboards § 111.30-27 Direct current ship's service switchboards. (a) Each direct current ship's service switchboard must have the equipment required by paragraphs (b...

46 CFR 111.30-27 - Direct current ship's service switchboards.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Direct current ship's service switchboards. 111.30-27... ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Switchboards § 111.30-27 Direct current ship's service switchboards. (a) Each direct current ship's service switchboard must have the equipment required by paragraphs (b...

46 CFR 131.530 - Abandon-ship training and drills.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Abandon-ship training and drills. 131.530 Section 131... OPERATIONS Tests, Drills, and Inspections § 131.530 Abandon-ship training and drills. (a) Material for... rescue gear (slings, baskets, stretchers) and vessel's line-throwing apparatus. (b) An abandon-ship drill...

46 CFR 131.530 - Abandon-ship training and drills.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Abandon-ship training and drills. 131.530 Section 131... OPERATIONS Tests, Drills, and Inspections § 131.530 Abandon-ship training and drills. (a) Material for... rescue gear (slings, baskets, stretchers) and vessel's line-throwing apparatus. (b) An abandon-ship drill...

46 CFR 131.530 - Abandon-ship training and drills.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Abandon-ship training and drills. 131.530 Section 131... OPERATIONS Tests, Drills, and Inspections § 131.530 Abandon-ship training and drills. (a) Material for... rescue gear (slings, baskets, stretchers) and vessel's line-throwing apparatus. (b) An abandon-ship drill...

46 CFR 111.10-9 - Ship's service supply transformers; two required.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Ship's service supply transformers; two required. 111.10... ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Power Supply § 111.10-9 Ship's service supply transformers; two required. If transformers are used to supply the ship's service distribution system required by...

46 CFR 111.10-9 - Ship's service supply transformers; two required.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Ship's service supply transformers; two required. 111.10... ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Power Supply § 111.10-9 Ship's service supply transformers; two required. If transformers are used to supply the ship's service distribution system required by...

46 CFR 111.10-9 - Ship's service supply transformers; two required.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Ship's service supply transformers; two required. 111.10... ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Power Supply § 111.10-9 Ship's service supply transformers; two required. If transformers are used to supply the ship's service distribution system required by...

46 CFR 111.10-9 - Ship's service supply transformers; two required.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Ship's service supply transformers; two required. 111.10... ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Power Supply § 111.10-9 Ship's service supply transformers; two required. If transformers are used to supply the ship's service distribution system required by...

46 CFR 111.10-9 - Ship's service supply transformers; two required.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Ship's service supply transformers; two required. 111.10... ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Power Supply § 111.10-9 Ship's service supply transformers; two required. If transformers are used to supply the ship's service distribution system required by...

44 CFR 402.5 - Forwarding commodities previously shipped.

Code of Federal Regulations, 2011 CFR

2011-10-01

... previously shipped. 402.5 Section 402.5 Emergency Management and Assistance DEPARTMENT OF COMMERCE AND DEPARTMENT OF TRANSPORTATION SHIPMENTS ON AMERICAN FLAG SHIPS AND AIRCRAFT (T-1, INT. 1) § 402.5 Forwarding commodities previously shipped. Order T-1 applies to transportation on or discharge from ships documented...

32 CFR 700.873 - Inspection incident to commissioning of ships.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 5 2011-07-01 2011-07-01 false Inspection incident to commissioning of ships... The Commanding Officer Special Circumstances/ships in Naval Stations and Shipyards § 700.873 Inspection incident to commissioning of ships. When a ship is to be commissioned, the authority designated to...

46 CFR 176.910 - Passenger Ship Safety Certificate.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Passenger Ship Safety Certificate. 176.910 Section 176..., as Amended (SOLAS) § 176.910 Passenger Ship Safety Certificate. (a) A vessel, which carries more than 12 passengers on an international voyage must have a valid SOLAS Passenger Ship Safety Certificate...

46 CFR 115.910 - Passenger Ship Safety Certificate.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Passenger Ship Safety Certificate. 115.910 Section 115... Ship Safety Certificate. (a) A vessel that carries more than 12 passengers on an international voyage must have a valid SOLAS Passenger Ship Safety Certificate. The Commandant authorizes the cognizant...

46 CFR 115.910 - Passenger Ship Safety Certificate.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Passenger Ship Safety Certificate. 115.910 Section 115... Ship Safety Certificate. (a) A vessel that carries more than 12 passengers on an international voyage must have a valid SOLAS Passenger Ship Safety Certificate. The Commandant issues the original SOLAS...

47 CFR 80.277 - Ship Security Alert System (SSAS).

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Ship Security Alert System (SSAS). 80.277... SERVICES STATIONS IN THE MARITIME SERVICES Equipment Authorization for Compulsory Ships § 80.277 Ship Security Alert System (SSAS). (a) Vessels equipped with a Ship Security Alert System pursuant to the Safety...

33 CFR 104.295 - Additional requirements-cruise ships.

Code of Federal Regulations, 2011 CFR

2011-07-01

... ships. 104.295 Section 104.295 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... requirements—cruise ships. (a) At all MARSEC Levels, the owner or operator of a cruise ship must ensure the... cruise ship must ensure that security briefs to passengers about the specific threat are provided. ...

7 CFR 987.62 - Reports of dates shipped.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Reports of dates shipped. 987.62 Section 987.62... dates shipped. Each handler who ships dates during a crop year shall submit to the Committee, in such... shipped by him and such other information pertinent thereto as the Committee may specify. ...

7 CFR 987.62 - Reports of dates shipped.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 8 2011-01-01 2011-01-01 false Reports of dates shipped. 987.62 Section 987.62... dates shipped. Each handler who ships dates during a crop year shall submit to the Committee, in such... shipped by him and such other information pertinent thereto as the Committee may specify. ...

46 CFR 169.817 - Master to instruct ship's company.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Master to instruct ship's company. 169.817 Section 169.817 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Operations § 169.817 Master to instruct ship's company. The master shall conduct drills and give...

46 CFR 169.817 - Master to instruct ship's company.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Master to instruct ship's company. 169.817 Section 169.817 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Operations § 169.817 Master to instruct ship's company. The master shall conduct drills and give...

46 CFR 115.910 - Passenger Ship Safety Certificate.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Passenger Ship Safety Certificate. 115.910 Section 115... Ship Safety Certificate. (a) A vessel that carries more than 12 passengers on an international voyage must have a valid SOLAS Passenger Ship Safety Certificate. The Commandant authorizes the cognizant...

46 CFR 176.910 - Passenger Ship Safety Certificate.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Passenger Ship Safety Certificate. 176.910 Section 176..., as Amended (SOLAS) § 176.910 Passenger Ship Safety Certificate. (a) A vessel, which carries more than 12 passengers on an international voyage must have a valid SOLAS Passenger Ship Safety Certificate...

46 CFR 115.910 - Passenger Ship Safety Certificate.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Passenger Ship Safety Certificate. 115.910 Section 115... Ship Safety Certificate. (a) A vessel that carries more than 12 passengers on an international voyage must have a valid SOLAS Passenger Ship Safety Certificate. The Commandant authorizes the cognizant...

46 CFR 176.910 - Passenger Ship Safety Certificate.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Passenger Ship Safety Certificate. 176.910 Section 176..., as Amended (SOLAS) § 176.910 Passenger Ship Safety Certificate. (a) A vessel, which carries more than 12 passengers on an international voyage must have a valid SOLAS Passenger Ship Safety Certificate...

32 CFR 700.873 - Inspection incident to commissioning of ships.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 5 2012-07-01 2012-07-01 false Inspection incident to commissioning of ships... The Commanding Officer Special Circumstances/ships in Naval Stations and Shipyards § 700.873 Inspection incident to commissioning of ships. When a ship is to be commissioned, the authority designated to...

47 CFR 80.277 - Ship Security Alert System (SSAS).

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Ship Security Alert System (SSAS). 80.277... SERVICES STATIONS IN THE MARITIME SERVICES Equipment Authorization for Compulsory Ships § 80.277 Ship Security Alert System (SSAS). (a) Vessels equipped with a Ship Security Alert System pursuant to the Safety...

46 CFR 115.910 - Passenger Ship Safety Certificate.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Passenger Ship Safety Certificate. 115.910 Section 115... Ship Safety Certificate. (a) A vessel that carries more than 12 passengers on an international voyage must have a valid SOLAS Passenger Ship Safety Certificate. The Commandant authorizes the cognizant...

44 CFR 402.5 - Forwarding commodities previously shipped.

Code of Federal Regulations, 2014 CFR

2014-10-01

... previously shipped. 402.5 Section 402.5 Emergency Management and Assistance DEPARTMENT OF COMMERCE AND DEPARTMENT OF TRANSPORTATION SHIPMENTS ON AMERICAN FLAG SHIPS AND AIRCRAFT (T-1, INT. 1) § 402.5 Forwarding commodities previously shipped. Order T-1 applies to transportation on or discharge from ships documented...

33 CFR 104.295 - Additional requirements-cruise ships.

Code of Federal Regulations, 2014 CFR

2014-07-01

... ships. 104.295 Section 104.295 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... requirements—cruise ships. (a) At all MARSEC Levels, the owner or operator of a cruise ship must ensure the... cruise ship must ensure that security briefs to passengers about the specific threat are provided. ...

44 CFR 402.5 - Forwarding commodities previously shipped.

Code of Federal Regulations, 2013 CFR

2013-10-01

... previously shipped. 402.5 Section 402.5 Emergency Management and Assistance DEPARTMENT OF COMMERCE AND DEPARTMENT OF TRANSPORTATION SHIPMENTS ON AMERICAN FLAG SHIPS AND AIRCRAFT (T-1, INT. 1) § 402.5 Forwarding commodities previously shipped. Order T-1 applies to transportation on or discharge from ships documented...

7 CFR 987.62 - Reports of dates shipped.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 8 2013-01-01 2013-01-01 false Reports of dates shipped. 987.62 Section 987.62... dates shipped. Each handler who ships dates during a crop year shall submit to the Committee, in such... shipped by him and such other information pertinent thereto as the Committee may specify. ...

46 CFR 169.817 - Master to instruct ship's company.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Master to instruct ship's company. 169.817 Section 169.817 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Operations § 169.817 Master to instruct ship's company. The master shall conduct drills and give...

47 CFR 80.277 - Ship Security Alert System (SSAS).

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Ship Security Alert System (SSAS). 80.277... SERVICES STATIONS IN THE MARITIME SERVICES Equipment Authorization for Compulsory Ships § 80.277 Ship Security Alert System (SSAS). (a) Vessels equipped with a Ship Security Alert System pursuant to the Safety...

46 CFR 176.910 - Passenger Ship Safety Certificate.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Passenger Ship Safety Certificate. 176.910 Section 176..., as Amended (SOLAS) § 176.910 Passenger Ship Safety Certificate. (a) A vessel, which carries more than 12 passengers on an international voyage must have a valid SOLAS Passenger Ship Safety Certificate...

7 CFR 987.62 - Reports of dates shipped.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 8 2012-01-01 2012-01-01 false Reports of dates shipped. 987.62 Section 987.62... dates shipped. Each handler who ships dates during a crop year shall submit to the Committee, in such... shipped by him and such other information pertinent thereto as the Committee may specify. ...

47 CFR 80.277 - Ship Security Alert System (SSAS).

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 5 2013-10-01 2013-10-01 false Ship Security Alert System (SSAS). 80.277... SERVICES STATIONS IN THE MARITIME SERVICES Equipment Authorization for Compulsory Ships § 80.277 Ship Security Alert System (SSAS). (a) Vessels equipped with a Ship Security Alert System pursuant to the Safety...

32 CFR 700.873 - Inspection incident to commissioning of ships.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 5 2014-07-01 2014-07-01 false Inspection incident to commissioning of ships... The Commanding Officer Special Circumstances/ships in Naval Stations and Shipyards § 700.873 Inspection incident to commissioning of ships. When a ship is to be commissioned, the authority designated to...

44 CFR 402.5 - Forwarding commodities previously shipped.

Code of Federal Regulations, 2012 CFR

2012-10-01

... previously shipped. 402.5 Section 402.5 Emergency Management and Assistance DEPARTMENT OF COMMERCE AND DEPARTMENT OF TRANSPORTATION SHIPMENTS ON AMERICAN FLAG SHIPS AND AIRCRAFT (T-1, INT. 1) § 402.5 Forwarding commodities previously shipped. Order T-1 applies to transportation on or discharge from ships documented...

33 CFR 104.295 - Additional requirements-cruise ships.

Code of Federal Regulations, 2012 CFR

2012-07-01

... ships. 104.295 Section 104.295 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... requirements—cruise ships. (a) At all MARSEC Levels, the owner or operator of a cruise ship must ensure the... cruise ship must ensure that security briefs to passengers about the specific threat are provided. ...

7 CFR 987.62 - Reports of dates shipped.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 8 2014-01-01 2014-01-01 false Reports of dates shipped. 987.62 Section 987.62... dates shipped. Each handler who ships dates during a crop year shall submit to the Committee, in such... shipped by him and such other information pertinent thereto as the Committee may specify. ...

46 CFR 169.817 - Master to instruct ship's company.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Master to instruct ship's company. 169.817 Section 169.817 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Operations § 169.817 Master to instruct ship's company. The master shall conduct drills and give...

46 CFR 169.817 - Master to instruct ship's company.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Master to instruct ship's company. 169.817 Section 169.817 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Operations § 169.817 Master to instruct ship's company. The master shall conduct drills and give...

33 CFR 104.295 - Additional requirements-cruise ships.

Code of Federal Regulations, 2013 CFR

2013-07-01

... ships. 104.295 Section 104.295 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... requirements—cruise ships. (a) At all MARSEC Levels, the owner or operator of a cruise ship must ensure the... cruise ship must ensure that security briefs to passengers about the specific threat are provided. ...

49 CFR 172.201 - Preparation and retention of shipping papers.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Preparation and retention of shipping papers. 172..., TRAINING REQUIREMENTS, AND SECURITY PLANS Shipping Papers § 172.201 Preparation and retention of shipping papers. (a) Contents. When a description of hazardous material is required to be included on a shipping...

46 CFR 153.976 - Transfer of packaged cargo or ship's stores.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Transfer of packaged cargo or ship's stores. 153.976 Section 153.976 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Operations Cargo...

46 CFR 153.976 - Transfer of packaged cargo or ship's stores.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Transfer of packaged cargo or ship's stores. 153.976 Section 153.976 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Operations Cargo...

46 CFR 153.976 - Transfer of packaged cargo or ship's stores.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Transfer of packaged cargo or ship's stores. 153.976 Section 153.976 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Operations Cargo...

46 CFR 153.976 - Transfer of packaged cargo or ship's stores.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Transfer of packaged cargo or ship's stores. 153.976 Section 153.976 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Operations Cargo...

Use of Heavy Lift Ships as Modular Casualty Receiving Ships

DTIC Science & Technology

2007-04-01

ship. • Combination product tanker and heavy lift ship. • Specialist dock or yacht transport ship. The M.V. Black Marlin, CombiDock, and the...regulations. • Pollution must be disposed of properly as set by various organizations. Pollutants include oil, noxious liquid substances in bulk, sewage ...pictured in Figure 21, is typically used to ship yachts from one location to another, and yacht owners have the option to travel with their yachts

46 CFR 167.15-15 - Application for inspection of a new nautical school ship or a conversion of a vessel to a...

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Application for inspection of a new nautical school ship or a conversion of a vessel to a nautical school ship. 167.15-15 Section 167.15-15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS PUBLIC NAUTICAL SCHOOL SHIPS Inspections § 167.15-15 Application for inspection of...

Ship's doctors qualifications required for cruise ships: Recruiter's comments on the German-Norwegian debate.

PubMed

Ottomann, Christian

2015-01-01

This contribution is intended to fertilise the current discussion of ship's doctors qualifications required for cruise ships. Therefore 10 points are added to the debate containing different considerations focussing on the recommendations of the German Society of Maritime Medicine, the American College of Emergency Physicians (ACEP's) Health Care Guidelines for Cruise Ship Medical Facilities and the different skills a ship's doctor should have from the perspective of the recruiter.

Numerical Simulation of Galvanic Corrosion Caused by Shaft Grounding Systems in Steel Ship Hulls

DTIC Science & Technology

2005-01-01

ship hull on paint holidays because of the substantial difference of the electric potentials between the steel ship hull and the nickel-aluminum...steel ship hull on paint holidays because of the substantial difference of the electric potentials between the steel ship hull and the nickel...substantial difference of the electric potentials between the steel ship hull and the nickel-aluminum bronze propellers. There are concerns on the

Gastrointestinal illness on passenger cruise ships, 1975-1978.

PubMed Central

Dannenberg, A L; Yashuk, J C; Feldman, R A

1982-01-01

Following investigations in 1972-1973 of outbreaks of enteric disease on cruise ships using American ports, a surveillance system was established which required that 24 hours before arrival in port, each ship report the number of persons with diarrheal illness seen by the ship's physician during the cruise. The reported data were found to be reliable; they established a baseline incidence for diarrhea on cruise ships. A significantly high portion of enteric disease outbreaks occurred on vessels that did not pass routine annual or semiannual sanitation inspections. The cruise ship sanitation program, developed with the cooperation of the cruise ship industry and the Centers for Disease Control, appears to have been successful in reducing the overall rate of cruise ship associated outbreaks of enteric illness. PMID:7065338

Potential effects of shipping on submersed macrophytes in the St. Clair and Detroit Rivers of the Great Lakes

USGS Publications Warehouse

Schloesser, Donald W.; Manny, Bruce A.

1989-01-01

An extensive survey of submersed macrophytes in the St. Clair and Detroit rivers revealed statistically significant differences in the composition and density of macrophyte beds in shipping channels (used by commercial vessels passing between Lakes Huron and Erie) and non-shipping channels. Of nine common macrophyte taxa, four (Characae, Potamogeton richardsonii, Potamogeton spp. narrow-leaf forms, and Najas flexilis) were found more frequently and three (Myriophyllum spicatum, Elodea canadensis, and Heteranthera dubia) less frequently in shipping than in non-shipping channels. In general, macrophyte beds were less dense in shipping channels than in non-shipping channels. We postulate that disruption of the prevailing unidirectional current patterns and erosion of suitable substrate or breakage of plant stems by passing vessels caused the observed differences in the composition and density of macrophyte beds in shipping and non-shipping channels in the St. Clair and Detroit rivers.

An Adaptive Ship Detection Scheme for Spaceborne SAR Imagery

PubMed Central

Leng, Xiangguang; Ji, Kefeng; Zhou, Shilin; Xing, Xiangwei; Zou, Huanxin

2016-01-01

With the rapid development of spaceborne synthetic aperture radar (SAR) and the increasing need of ship detection, research on adaptive ship detection in spaceborne SAR imagery is of great importance. Focusing on practical problems of ship detection, this paper presents a highly adaptive ship detection scheme for spaceborne SAR imagery. It is able to process a wide range of sensors, imaging modes and resolutions. Two main stages are identified in this paper, namely: ship candidate detection and ship discrimination. Firstly, this paper proposes an adaptive land masking method using ship size and pixel size. Secondly, taking into account the imaging mode, incidence angle, and polarization channel of SAR imagery, it implements adaptive ship candidate detection in spaceborne SAR imagery by applying different strategies to different resolution SAR images. Finally, aiming at different types of typical false alarms, this paper proposes a comprehensive ship discrimination method in spaceborne SAR imagery based on confidence level and complexity analysis. Experimental results based on RADARSAT-1, RADARSAT-2, TerraSAR-X, RS-1, and RS-3 images demonstrate that the adaptive scheme proposed in this paper is able to detect ship targets in a fast, efficient and robust way. PMID:27563902

Manipulating ship fuel sulfur content and modeling the effects on air quality and climate

NASA Astrophysics Data System (ADS)

Partanen, Antti-Ilari; Laakso, Anton; Schmidt, Anja; Kokkola, Harri; Kuokkanen, Tuomas; Kerminen, Veli-Matti; Lehtinen, Kari E. J.; Laakso, Lauri; Korhonen, Hannele

2013-04-01

Aerosol emissions from international shipping are known to cause detrimental health effects on people mainly via increased lung cancer and cardiopulmonary diseases. On the other hand, the aerosol particles from the ship emissions modify the properties of clouds and are believed to have a significant cooling effect on the global climate. In recent years, aerosol emissions from shipping have been more strictly regulated in order to improve air quality and thus decrease the mortality due to ship emissions. Decreasing the aerosol emissions from shipping is projected to decrease their cooling effect, which would intensify the global warming even further. In this study, we use a global aerosol-climate model ECHAM5.5-HAM2 to test if continental air quality can be improved while still retaining the cooling effect from shipping. The model explicitly resolves emissions of aerosols and their pre-cursor gases. The model also calculates the interaction between aerosol particles and clouds, and can thus predict the changes in cloud properties due to aerosol emissions. We design and simulate a scenario where ship fuel sulfur content is strictly limited to 0.1% near all coastal regions, but doubled in the open oceans from the current global mean value of 2.7% (geo-ships). This scenario is compared to three other simulations: 1) No shipping emissions at all (no-ships), 2) present-day shipping emissions (std-ships) and 3) a future scenario where sulfur content is limited to 0.1% in the coastal zones and to 0.5% in the open ocean (future-ships). Global mean radiative flux perturbation (RFP) in std-ships compared to no-ships is calculated to be -0.4 W m-2, which is in the range of previous estimates for present-day shipping emissions. In the geo-ships simulation the corresponding global mean RFP is roughly equal, but RFP is spatially distributed more on the open oceans, as expected. In future-ships the decreased aerosol emissions provide weaker cooling effect of only -0.1 W m-2. In order to assess the health effects of different emission scenarios we diagnose PM2.5 concentrations from each simulation. Then, we use PM2.5 concentrations and C-R functions to calculate the changes in mortality related to lung cancer and cardiopulmonary diseases. Our preliminary analysis suggests that mortality in geo-ships would be lower than in std-ships. Strict sulfur content limits also in the open oceans (future-ships) would decrease the mortality even more. Idea of deliberately increasing fuel sulfur content in order to produce a cooling effect can be classified as one form of solar radiation management (SRM). There are several scientific, ethical and political problems associated with SRM technologies and a number of them would be applicable to the idea we present here. For example, there would be a conflict between the existing international treaties and the proposed increase in ship fuel sulfur content. However, our study is increasing the knowledge of air quality and climate trade-offs related to ship emission controls. If the cooling effect of ship traffic is considered too precious to lose, there might be ways to preserve it, while still notably increasing the continental air quality.

A Regulatory Role for Src Homology 2 Domain–Containing Inositol 5′-Phosphatase (Ship) in Phagocytosis Mediated by Fcγ Receptors and Complement Receptor 3 (αMβ2; Cd11b/Cd18)

PubMed Central

Cox, Dianne; Dale, Benjamin M.; Kashiwada, Masaki; Helgason, Cheryl D.; Greenberg, Steven

2001-01-01

The Src homology 2 domain–containing inositol 5′-phosphatase (SHIP) is recruited to immunoreceptor tyrosine-based inhibition motif (ITIM)–containing proteins, thereby suppressing phosphatidylinositol 3-kinase (PI 3-kinase)–dependent pathways. The role of SHIP in phagocytosis, a PI 3-kinase–dependent pathway, is unknown. Overexpression of SHIP in macrophages led to an inhibition of phagocytosis mediated by receptors for the Fc portion of IgG (FcγRs). In contrast, macrophages expressing catalytically inactive SHIP or lacking SHIP expression demonstrated enhanced phagocytosis. To determine whether SHIP regulates phagocytosis mediated by receptors that are not known to recruit ITIMs, we determined the effect of SHIP expression on complement receptor 3 (CR3; CD11b/CD18; αMβ2)–dependent phagocytosis. Macrophages overexpressing SHIP demonstrated impaired CR3-mediated phagocytosis, whereas macrophages expressing catalytically inactive SHIP demonstrated enhanced phagocytosis. CR3-mediated phagocytosis in macrophages derived from SHIP−/− mice was up to 2.5 times as efficient as that observed in macrophages derived from littermate controls. SHIP was localized to FcγR- and CR3-containing phagocytic cups and was recruited to the cytoskeleton upon clustering of CR3. In a transfected COS cell model of activation-independent CR3-mediated phagocytosis, catalytically active but not inactive SHIP also inhibited phagocytosis. We conclude that PI 3-kinase(s) and SHIP regulate multiple forms of phagocytosis and that endogenous SHIP plays a role in modulating β2 integrin outside-in signaling. PMID:11136821

Influenza Outbreaks Among Passengers and Crew on Two Cruise Ships: A Recent Account of Preparedness and Response to an Ever-Present Challenge.

PubMed

Millman, Alexander J; Kornylo Duong, Krista; Lafond, Kathryn; Green, Nicole M; Lippold, Susan A; Jhung, Michael A

2015-01-01

During spring 2014, two large influenza outbreaks occurred among cruise ship passengers and crew on trans-hemispheric itineraries. Passenger and crew information for both ships was obtained from components of the ship medical records. Data included demographics, diagnosis of influenza-like illness (ILI) or acute respiratory illness (ARI), illness onset date, passenger cabin number, crew occupation, influenza vaccination history, and rapid influenza diagnostic test (RIDT) result, if performed. In total, 3.7% of passengers and 3.1% of crew on Ship A had medically attended acute respiratory illness (MAARI). On Ship B, 6.2% of passengers and 4.7% of crew had MAARI. In both outbreaks, passengers reported illness prior to the ship's departure. Influenza activity was low in the places of origin of the majority of passengers and both ships' ports of call. The median age of affected passengers on both ships was 70 years. Diagnostic testing revealed three different co-circulating influenza viruses [influenza A(H1N1)pdm09, influenza A(H3N2), and influenza B] on Ship A and one circulating influenza virus (influenza B) on Ship B. Both ships voluntarily reported the outbreaks to the Centers for Disease Control and Prevention (CDC) and implemented outbreak response plans including isolation of sick individuals and antiviral treatment and prophylaxis. Influenza activity can become widespread during cruise ship outbreaks and can occur outside of traditional influenza seasons. Comprehensive outbreak prevention and control plans, including prompt antiviral treatment and prophylaxis, may mitigate the impact of influenza outbreaks on cruise ships. Published 2015. This article is a U.S. Government work and is in the public domain in the USA.

Ship emission inventory and its impact on the PM2.5 air pollution in Qingdao Port, North China

NASA Astrophysics Data System (ADS)

Chen, Dongsheng; Wang, Xiaotong; Nelson, Peter; Li, Yue; Zhao, Na; Zhao, Yuehua; Lang, Jianlei; Zhou, Ying; Guo, Xiurui

2017-10-01

In this study, a first high temporal-spatial ship emission inventory in Qingdao Port and its adjacent waters has been developed using a ;bottom-up; method based on Automatic Identification System (AIS) data. The total estimated ship emissions for SO2, NOX, PM10, PM2.5, HC and CO in 2014 are 3.32 × 104, 4.29 × 104, 4.54 × 103, 4.18 × 103, 1.85 × 103 and 3.66 × 103 tonnes, respectively. Emissions of SO2 and NOX from ships account for 9% and 13% of the anthropogenic totals in Qingdao, respectively. The main contributors to the ship emissions are containers, followed by fishing ships, oil tankers and bulk carriers. The inter-monthly ship emissions varied significantly due to two reasons: stopping of the fishing ship activities during the fishing moratorium and the reduction of freight volume around the Chinese New Year Festival. Emissions from transport vessels concentrated basically along the shipping routes, while fishing ships contributed to massive irregular spatial emissions in the sea. The impact of ship emissions on the ambient air quality was further investigated using the Weather Research and Forecasting with Chemistry (WRF/Chem) model. The results reveal that the contribution of ship emissions to the PM2.5 concentrations in Qingdao is the highest in summer (13.1%) and the lowest in winter (1.5%). The impact was more evident over densely populated urban areas, where the contributions from ship emissions could be over 20% in July due to their close range to the docks. These results indicated that the management and control of the ship emissions are highly demanded considering their remarkable influence on the air quality and potential negative effects on human health.

Update on emissions and environmental impacts from the international fleet of ships: the contribution from major ship types and ports

NASA Astrophysics Data System (ADS)

Dalsøren, S. B.; Eide, M. S.; Endresen, Ø.; Mjelde, A.; Gravir, G.; Isaksen, I. S. A.

2009-03-01

A reliable and up-to-date ship emission inventory is essential for atmospheric scientists quantifying the impact of shipping and for policy makers implementing regulations and incentives for emission reduction. The emission modelling in this study takes into account ship type and size dependent input data for 15 ship types and 7 size categories. Global port arrival and departure data for more than 32 000 merchant ships are used to establish operational profiles for the ship segments. The modelled total fuel consumption amounts to 217 Mt in 2004 of which 11 Mt is consumed in in-port operations. This is in agreement with international sales statistics. The modelled fuel consumption is applied to develop global emission inventories for CO2, NO2, SO2, CO, CH4, VOC (Volatile Organic Compounds), N2O, BC (Black Carbon) and OC (Organic Carbon). The global emissions from ships at sea and in ports are distributed geographically, applying extended geographical data sets covering about 2 million global ship observations and global port data for 32 000 ships. In addition to inventories for the world fleet, inventories are produced separately for the three dominating ship types, using ship type specific emission modelling and traffic distributions. A global Chemical Transport Model (CTM) was used to calculate the environmental impacts of the emissions. We find that ship emissions is a dominant contributor over much of the world oceans to surface concentrations of NO2 and SO2. The contribution is also large over some coastal zones. For surface ozone the contribution is high over the oceans but clearly also of importance over Western North America (contribution 15-25%) and Western Europe (5-15%). The contribution to tropospheric column ozone is up to 5-6%. The overall impact of ship emissions on global methane lifetime is large due to the high NOx emissions. With regard to acidification we find that ships contribute 11% to nitrate wet deposition and 4.5% to sulphur wet deposition globally. In certain coastal regions the contributions may be in the range 15-50%. In general we find that ship emissions have a large impact on acidic deposition and surface ozone in Western North America, Scandinavia, Western Europe, western North Africa and Malaysia/Indonesia. For most of these regions container traffic, the largest emitter by ship type, has the largest impact. This is the case especially for the Pacific and the related container trade routes between Asia and North America. However, the contributions from bulk ships and tank vessels are also significant in the above mentioned impact regions. Though the total ship impact at low latitudes is lower, the tank vessels have a quite large contribution at low latitudes and near the Gulf of Mexico and Middle East. The bulk ships are characterized by large impact in Oceania compared to other ship types. In Scandinavia and north-Western Europe, one of the major ship impact regions, the three largest ship types have rather small relative contributions. The impact in this region is probably dominated by smaller ships operating closer to the coast. For emissions in ports impacts on NO2 and SO2 seem to be of significance. For most ports the contribution to the two components is in the range 0.5-5%, for a few ports it exceeds 10%. The approach presented provides an improvement in characterizing fleet operational patterns, and thereby ship emissions and impacts. Furthermore, the study shows where emission reductions can be applied to most effectively minimize the impacts by different ship types.

Update on emissions and environmental impacts from the international fleet of ships. The contribution from major ship types and ports

NASA Astrophysics Data System (ADS)

Dalsøren, S. B.; Eide, M. S.; Endresen, Ø.; Mjelde, A.; Gravir, G.; Isaksen, I. S. A.

2008-10-01

A reliable and up-to-date ship emission inventory is essential for atmospheric scientists quantifying the impact of shipping and for policy makers implementing regulations and incentives for emission reduction. The emission modelling in this study takes into account ship type and size dependent input data for 15 ship types and 7 size categories. Global port arrival and departure data for more than 32 000 merchant ships are used to establish operational profiles for the ship segments. The modelled total fuel consumption amounts to 217 Mt in 2004 of which 11 Mt is consumed in in-port operations. This is in agreement with international sales statistics. The modelled fuel consumption is applied to develop global emission inventories for CO2, NO2, SO2, CO, CH4, VOC (Volatile Organic Compounds), N2O, BC (Black Carbon) and OC (Organic Carbon). The global emissions from ships at sea and in ports are distributed geographically, applying extended geographical data sets covering about 2 million global ship observations and global port data for 32 000 ships. In addition to inventories for the world fleet, inventories are produced separately for the three dominating ship types, using ship type specific emission modelling and traffic distributions. A global Chemical Transport Model (CTM) was used to calculate the environmental impacts of the emissions. We find that ship emissions is a dominant contributor over much of the world oceans to surface concentrations of NO2 and SO2. The contribution is also large over some coastal zones. For surface ozone the contribution is high over the oceans but clearly also of importance over western North America (contribution 15 25%) and western Europe (5 15%). The contribution to tropospheric column ozone is up to 5 6%. The overall impact of ship emissions on global methane lifetime is large due to the high NOx emissions. With regard to acidification we find that ships contribute 11% to nitrate wet deposition and 4.5% to sulphur wet deposition globally. In certain coastal regions the contributions may be in the range 15 50%. In general we find that ship emissions have a large impact on acidic deposition and surface ozone in western North America, Scandinavia, western Europe, western North Africa and Malaysia/Indonesia. For most of these regions container traffic, the largest emitter by ship type, has the largest impact. This is the case especially for the Pacific and the related container trade routes between Asia and North America. However, the contributions from bulk ships and tank vessels are also significant in the above mentioned impact regions. Though the total ship impact at low latitudes is lower, the tank vessels have a quite large contribution at low latitudes and near the Gulf of Mexico and Middle East. The bulk ships are characterized by large impact in Oceania compared to other ship types. In Scandinavia and north-western Europe, one of the major ship impact regions, the three largest ship types have rather small relative contributions. The impact in this region is probably dominated by smaller ships operating closer to the coast. For emissions in ports impacts on NO2 and SO2 seem to be of significance. For most ports the contribution to the two components is in the range 0.5 5%, for a few ports it exceeds 10%. The approach presented provides an improvement in characterizing fleet operational patterns, and thereby ship emissions and impacts. Furthermore, the study shows where emission reductions can be applied to most effectively minimize the impacts by different ship types.

A Small Molecule Polyamine Oxidase Inhibitor Blocks Androgen-Induced Oxidative Stress and Delays Prostate Cancer Progression in the TRAMP Mouse Model

PubMed Central

Basu, Hirak S.; Thompson, Todd A.; Church, Dawn R.; Clower, Cynthia C.; Mehraein-Ghomi, Farideh; Amlong, Corey A.; Martin, Christopher T.; Woster, Patrick M.; Lindstrom, Mary J.; Wilding, George

2009-01-01

High levels of reactive oxygen species (ROS) present in human prostate epithelia are an important etiological factor in prostate cancer (CaP) occurrence, recurrence and progression. Androgen induces ROS production in the prostate by a yet unknown mechanism. Here, to the best of our knowledge, we report for the first time that androgen induces an overexpression of spermidine/spermine N1-acetyltransferase (SSAT), the rate-limiting enzyme in the polyamine oxidation pathway. As prostatic epithelia produce a large excess of polyamines, the androgen-induced polyamine oxidation that produces H2O2 could be a major reason for the high ROS levels in the prostate epithelia. A small molecule polyamine oxidase inhibitor N,N'-butanedienyl butanediamine (MDL 72,527 or CPC-200) effectively blocks androgen-induced ROS production in human CaP cells as well as significantly delays CaP progression and death in animals developing spontaneous CaP. These data demonstrate that polyamine oxidation is not only a major pathway for ROS production in prostate, but inhibiting this pathway also successfully delays prostate cancer progression. PMID:19773450

Simultaneous Evaporation of Cu and Sn from Liquid Steel

NASA Astrophysics Data System (ADS)

Jung, Sung-Hoon; Kang, Youn-Bae

2016-08-01

In order to understand evaporation refining of tramp elements in molten ferrous scrap, Cu and Sn, a series of experiments were carried out using liquid-gas reaction in a levitation melting equipment. Effect of S and C, which are abundant in hot metal from ironmaking process, was examined and analyzed by employing a comprehensive evaporation kinetic model developed by the present authors (Jung et al. in Metall Mater Trans B 46B:250-258, 2014; Jung et al. in Metall Mater Trans B 46B:259-266, 2014; Jung et al. in Metall Mater Trans B 46B:267-277, 2014; Jung and Kang in Metall Mater Trans B 10.1007/s11663-016-0601-5, 2016). Evaporation of Cu and Sn were treated by evaporation of individual species such as Cu(g), CuS(g), Sn(g), and SnS(g), along with CS2(g). Decrease of Cu and Sn content in liquid steel was in good agreement with the model prediction. Optimum conditions of steel composition for the rapid evaporation of Cu and Sn were proposed by utilizing the model predictions.

Activating Akt1 mutations alter DNA double strand break repair and radiosensitivity

PubMed Central

Oeck, S.; Al-Refae, K.; Riffkin, H.; Wiel, G.; Handrick, R.; Klein, D.; Iliakis, G.; Jendrossek, V.

2017-01-01

The survival kinase Akt has clinical relevance to radioresistance. However, its contributions to the DNA damage response, DNA double strand break (DSB) repair and apoptosis remain poorly defined and often contradictory. We used a genetic approach to explore the consequences of genetic alterations of Akt1 for the cellular radiation response. While two activation-associated mutants with prominent nuclear access, the phospho-mimicking Akt1-TDSD and the clinically relevant PH-domain mutation Akt1-E17K, accelerated DSB repair and improved survival of irradiated Tramp-C1 murine prostate cancer cells and Akt1-knockout murine embryonic fibroblasts in vitro, the classical constitutively active membrane-targeted myrAkt1 mutant had the opposite effects. Interestingly, DNA-PKcs directly phosphorylated Akt1 at S473 in an in vitro kinase assay but not vice-versa. Pharmacological inhibition of DNA-PKcs or Akt restored radiosensitivity in tumour cells expressing Akt1-E17K or Akt1-TDSD. In conclusion, Akt1-mediated radioresistance depends on its activation state and nuclear localization and is accessible to pharmacologic inhibition. PMID:28209968

Optoacoustic imaging of an animal model of prostate cancer

NASA Astrophysics Data System (ADS)

Patterson, Michelle P.; Arsenault, Michel; Riley, Chris; Kolios, Michael; Whelan, William M.

2010-02-01

Prostate cancer is currently the most common cancer among Canadian men. Due to an increase in public awareness and screening, prostate cancer is being detected at earlier stages and in much younger men. This is raising the need for better treatment monitoring approaches. Optoacoustic imaging is a new technique that involves exposing tissues to pulsed light and detecting the acoustic waves generated by the tissue. Optoacoustic images of a tumour bearing mouse and an agematched control were acquired for a 775 nm illumination using a reverse-mode imaging system. A murine model of prostate cancer, TRAMP (transgenetic adenocarcinoma of mouse prostate), was investigated. The results show an increase in optoacoustic signal generated by the tumour compared to that generated by the surrounding tissues with a contrast ratio of 3.5. The dimensions of the tumour in the optoacoustic image agreed with the true tumour dimensions to within 0.5 mm. In this study we show that there are detectable changes in optoacoustic signal strength that arise from the presence of a tumour in the prostate, which demonstrates the potential of optoacoustic imaging for the monitoring of prostate cancer therapy.

Dendritic Cells Program Non-Immunogenic Prostate-Specific T Cell Responses Beginning at Early Stages of Prostate Tumorigenesis

PubMed Central

Mihalyo, Marianne A.; Hagymasi, Adam T.; Slaiby, Aaron M.; Nevius, Erin E.; Adler, Adam J.

2010-01-01

BACKGROUND Prostate cancer promotes the development of T cell tolerance towards prostatic antigens, potentially limiting the efficacy of prostate cancer vaccines targeting these antigens. Here, we sought to determine the stage of disease progression when T cell tolerance develops, as well as the role of steady state dendritic cells (DC) and CD4+CD25+ T regulatory cells (Tregs) in programming tolerance. METHODS The response of naïve HA-specific CD4+ T cells were analyzed following adoptive transfer into Pro-HA × TRAMP transgenic mice harboring variably-staged HA-expressing prostate tumors on two genetic backgrounds that display different patterns and kinetics of tumorigenesis. The role of DC and Tregs in programming HA-specific CD4 cell responses were assessed via depletion. RESULTS HA-specific CD4 cells underwent non-immunogenic responses at all stages of tumorigenesis in both genetic backgrounds. These responses were completely dependent on DC, but not appreciably influenced by Tregs. CONCLUSIONS These results suggest that tolerogenicity is an early and general property of prostate tumors. PMID:17221844

Turbulent AGN tori .

NASA Astrophysics Data System (ADS)

Schartmann, M.; Meisenheimer, K.; Klahr, H.; Camenzind, M.; Wolf, S.; Henning, Th.

Recently, the MID-infrared Interferometric instrument (MIDI) at the VLTI has shown that dust tori in the two nearby Seyfert galaxies NGC 1068 and the Circinus galaxy are geometrically thick and can be well described by a thin, warm central disk, surrounded by a colder and fluffy torus component. By carrying out hydrodynamical simulations with the help of the TRAMP code \\citep{schartmann_Klahr_99}, we follow the evolution of a young nuclear star cluster in terms of discrete mass-loss and energy injection from stellar processes. This naturally leads to a filamentary large scale torus component, where cold gas is able to flow radially inwards. The filaments open out into a dense and very turbulent disk structure. In a post-processing step, we calculate observable quantities like spectral energy distributions or images with the help of the 3D radiative transfer code MC3D \\citep{schartmann_Wolf_03}. Good agreement is found in comparisons with data due to the existence of almost dust-free lines of sight through the large scale component and the large column densities caused by the dense disk.

Terrorism in Spain: emergency medical aspects.

PubMed

García-Castrillo Riesgo, Luis; García Merino, Antonio

2003-01-01

A terrorism movement has been active in Spain during the last 20 years, with a painful number of victims. Civil Defense is in charge of the coordination of all the structures that are implicated in a terrorist incident. There are three typical patterns of attacks: (1) individual attacks; (2) group attacks; and (3) mass attacks. The individual attacks are done with guns, usually 9 mm, fired from a short distance; victims die from serious intracranial damage. Collective attacks are done using explosives under vehicles, tramp bombs, or "bomb vehicles;" victims are of different severity with wounds, burns, and blast injuries. With mass attacks with "bomb vehicles" in buildings or crowded public places, the numbers of victims are elevated and produce brutal social consequences. Emergency Medical Services integrated in to "Civil Defense" try to minimize the damage by initializing treatment on-scene and with the rapid provision of definitive care. During the last year, post-traumatic stress disorder treatment groups have been providing care to the victims and personnel. Chemical or biological weapons have not been used, although this is a great concern to the authorities.

Deregulation of MiR-34b/Sox2 Predicts Prostate Cancer Progression.

PubMed

Forno, Irene; Ferrero, Stefano; Russo, Maria Veronica; Gazzano, Giacomo; Giangiobbe, Sara; Montanari, Emanuele; Del Nero, Alberto; Rocco, Bernardo; Albo, Giancarlo; Languino, Lucia R; Altieri, Dario C; Vaira, Valentina; Bosari, Silvano

2015-01-01

Most men diagnosed with prostate cancer will have an indolent and curable disease, whereas approximately 15% of these patients will rapidly progress to a castrate-resistant and metastatic stage with high morbidity and mortality. Therefore, the identification of molecular signature(s) that detect men at risk of progressing disease remains a pressing and still unmet need for these patients. Here, we used an integrated discovery platform combining prostate cancer cell lines, a Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP) model and clinically-annotated human tissue samples to identify loss of expression of microRNA-34b as consistently associated with prostate cancer relapse. Mechanistically, this was associated with epigenetics silencing of the MIR34B/C locus and increased DNA copy number loss, selectively in androgen-dependent prostate cancer. In turn, loss of miR-34b resulted in downstream deregulation and overexpression of the "stemness" marker, Sox2. These findings identify loss of miR-34b as a robust biomarker for prostate cancer progression in androgen-sensitive tumors, and anticipate a potential role of progenitor/stem cell signaling in this stage of disease.

Navy Littoral Combat Ship (LCS) Program: Oversight Issues and Options for Congress

DTIC Science & Technology

2007-06-11

Summary The Littoral Combat Ship (LCS) is a small, fast ship that uses modular “plug- and-fight” mission packages, including unmanned vehicles (UVs). The...small, fast ship that uses modular “plug-and- fight” mission packages, including unmanned vehicles (UVs). The basic version of the LCS, without any...including unmanned vehicles (UVs). Rather than being a multimission ship like the Navy’s current large surface combatants, the LCS is a focused-mission ship

Benchmarking Naval Shipbuilding with 3D Laser Scanning, Additive Manufacturing, and Collaborative Product Lifecycle Management

DTIC Science & Technology

2015-09-20

battle force ships . The changes affect a small number of ship classes designated as (very) small combatants or logistics and support ships . Specifically...accurate, and fast method of helping shipbuilders and manufacturers design , redesign, modify, and salvage ships . However, only a handful of several... ship construction to become a fleet of 306 battle force ships over the next 30 years. It is critical that the Navy capture the full benefits of new

Advanced Whale Detection Methods to Improve Whale-Ship Collision Avoidance

NASA Astrophysics Data System (ADS)

McGillivary, P. A.; Tougher, B.

2010-12-01

Collisions between whales and ships are now estimated to account for fully a third of all whale deaths worldwide. Such collisions can incur costly ship repairs, and may damage or disable ship steering requiring costly response efforts from state and federal agencies. While collisions with rare whale species are problematic in further reducing their low population numbers, collisions with some of the more abundant whale species are also becoming more common as their populations increase. The problem is compounded as ship traffic likewise continues to grow, thus posing a growing risk to both whales and ships. Federal agencies are considering policies to alter shipping lanes to minimize whale-ship collisions off California and elsewhere. Similar efforts have already been undertaken for the Boston Harbor ship approach, where a bend in the shipping lane was introduced to reduce ship traffic through a favorite area of the highly endangered North Atlantic Right Whale. The Boston shipping approach lane was also flanked with a system of moorings with whale detection hydrophones which broadcast the presence of calling whales in or near the ship channel to approaching ships in real time. When so notified, ships can post lookouts to avoid whale collisions, and reduce speed to reduce the likelihood of whale death, which is highly speed dependent. To reduce the likelihood and seriousness of whale-ship collisions off California and Alaska in particular, there is a need to better know areas of particularly high use by whales, and consider implementation of reduced ship speeds in these areas. There is also an ongoing discussion of altering shipping lanes in the Santa Barbara Channel to avoid habitual Blue whales aggregation areas in particular. However, unlike the case for Boston Harbor, notification of ships that whales are nearby to reduce or avoid collisions is complicated because many California and Alaska whale species do not call regularly, and would thus be undetected by hydrophone arrays. We here discuss the possibility of using Ambient Noise Imaging (ANI) systems initially developed for location of non-calling sperm whales along high speed ferry routes in the Canary Islands. A ‘hybrid’ ANI system has also been developed which uses sound from calling whales to ‘illuminate’ non-calling whales. Such systems designed for sperm whales would require modification for Blue and fin whales along California shipping lanes, and Bowhead whales in Alaska. We discuss how ANI whale detection systems could be developed for California and Alaska by combining bottom moorings with autonomous underwater vehicles (AUVs) and autonomous surface vehicles (ASVs) as part of ocean observing systems. The mechanisms, challenges, and potential solutions for use of ANI whale detection systems along critical shipping lanes along the California and Alaska coast to reduce whale-ship collisions are discussed as a means that permit science to assist in development of integrated state and federal ocean management policies. The combination of new scientific technology with ocean policy decisions can improve coastal ocean management, improve the safety and reduce the cost of shipping, while at the same time protecting endangered whale species.

75 FR 66643 - Importation of Mexican Hass Avocados; Additional Shipping Options

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-29

...-0016] RIN 0579-AD15 Importation of Mexican Hass Avocados; Additional Shipping Options AGENCY: Animal... States by adding the option to ship avocados to the United States in bulk shipping bins when safeguarding... Mexico and inquiries from a U.S. maritime port. These actions will allow additional options for shipping...

46 CFR 91.60-15 - Cargo Ship Safety Radio Certificate.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Cargo Ship Safety Radio Certificate. 91.60-15 Section 91..., 1974 § 91.60-15 Cargo Ship Safety Radio Certificate. Every vessel equipped with a radio installation on an international voyage must have a Cargo Ship Safety Radio Certificate. Each radio installation must...

46 CFR 176.910 - Passenger Ship Safety Certificate.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Passenger Ship Safety Certificate. 176.910 Section 176.910 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) INSPECTION AND CERTIFICATION International Convention for Safety of Life at Sea, 1974, as Amended (SOLAS) § 176.910 Passenger Ship...

Navy Littoral Combat Ship (LCS)/Frigate Program: Background and Issues for Congress

DTIC Science & Technology

2015-12-22

the ships. The Navy continues to review manning to determine appropriate levels, and is adding 20 berths to all seaframes. The increased berthing ...ships would determine the allocation of the three FY2010 ships, with the winning team getting two of the FY2010 ships and the other team getting one

46 CFR 71.75-5 - Passenger Ship Safety Certificate.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 3 2011-10-01 2011-10-01 false Passenger Ship Safety Certificate. 71.75-5 Section 71.75... Passenger Ship Safety Certificate. (a) All vessels on or certificated for an international voyage are required to have a “ SOLAS Passenger Ship Safety Certificate.” (b) All such vessels shall meet the...

7 CFR 927.8 - Ship or handle.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Ship or handle. 927.8 Section 927.8 Agriculture... Order Regulating Handling Definitions § 927.8 Ship or handle. Ship or handle means to sell, deliver, consign, transport or ship pears within the production area or between the production area and any point...

46 CFR 189.60-10 - Cargo Ship Safety Equipment Certificate.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Cargo Ship Safety Equipment Certificate. 189.60-10..., 1974 § 189.60-10 Cargo Ship Safety Equipment Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Equipment Certificate. (b) All such vessels shall meet the...

47 CFR 80.1201 - Special provisions for cable-repair ship stations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Special provisions for cable-repair ship... Radiodetermination § 80.1201 Special provisions for cable-repair ship stations. (a) A ship station may be authorized... equipment attached to the cable-marker buoy associated with the ship station must be described in the...

46 CFR 91.60-15 - Cargo Ship Safety Radio Certificate.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Cargo Ship Safety Radio Certificate. 91.60-15 Section 91..., 1974 § 91.60-15 Cargo Ship Safety Radio Certificate. Every vessel equipped with a radio installation on an international voyage must have a Cargo Ship Safety Radio Certificate. Each radio installation must...

46 CFR 91.60-10 - Cargo Ship Safety Equipment Certificate.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Cargo Ship Safety Equipment Certificate. 91.60-10..., 1974 § 91.60-10 Cargo Ship Safety Equipment Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Equipment Certificate. (b) All such vessels shall meet the applicable...

47 CFR 80.116 - Radiotelephone operating procedures for ship stations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Radiotelephone operating procedures for ship... Procedures-Ship Stations § 80.116 Radiotelephone operating procedures for ship stations. (a) Calling coast stations. (1) Use by ship stations of the frequency 2182 kHz for calling coast stations and for replying to...

46 CFR 91.60-5 - Cargo Ship Safety Construction Certificate.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Cargo Ship Safety Construction Certificate. 91.60-5..., 1974 § 91.60-5 Cargo Ship Safety Construction Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Construction Certificate. This certificate shall be issued by...

47 CFR 80.1123 - Watch requirements for ship stations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Watch requirements for ship stations. 80.1123... Procedures for Distress and Safety Communications § 80.1123 Watch requirements for ship stations. (a) While at sea, all ships must maintain a continuous watch: (1) On VHF DSC channel 70, if the ship is fitted...

46 CFR 91.60-10 - Cargo Ship Safety Equipment Certificate.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Cargo Ship Safety Equipment Certificate. 91.60-10..., 1974 § 91.60-10 Cargo Ship Safety Equipment Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Equipment Certificate. (b) All such vessels shall meet the applicable...

46 CFR 188.10-73 - Ships' stores and supplies.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Ships' stores and supplies. 188.10-73 Section 188.10-73 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS GENERAL PROVISIONS Definition of Terms Used in This Subchapter § 188.10-73 Ships' stores and supplies. This term...

47 CFR 80.1201 - Special provisions for cable-repair ship stations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Special provisions for cable-repair ship... Radiodetermination § 80.1201 Special provisions for cable-repair ship stations. (a) A ship station may be authorized... equipment attached to the cable-marker buoy associated with the ship station must be described in the...

7 CFR 927.8 - Ship or handle.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 8 2011-01-01 2011-01-01 false Ship or handle. 927.8 Section 927.8 Agriculture... Order Regulating Handling Definitions § 927.8 Ship or handle. Ship or handle means to sell, deliver, consign, transport or ship pears within the production area or between the production area and any point...

33 CFR 169.130 - When are ships required to make reports?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false When are ships required to make... SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY SHIP REPORTING SYSTEMS Establishment of Two Mandatory Ship Reporting Systems for the Protection of Northern Right Whales § 169.130 When are ships required to make...

46 CFR 91.60-5 - Cargo Ship Safety Construction Certificate.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Cargo Ship Safety Construction Certificate. 91.60-5..., 1974 § 91.60-5 Cargo Ship Safety Construction Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Construction Certificate. This certificate shall be issued by...

33 CFR 169.215 - How must a ship transmit position reports?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false How must a ship transmit position... SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY SHIP REPORTING SYSTEMS Transmission of Long Range Identification and Tracking Information § 169.215 How must a ship transmit position reports? A ship must transmit...

47 CFR 80.1073 - Radio operator requirements for ship stations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Radio operator requirements for ship stations...) General Provisions § 80.1073 Radio operator requirements for ship stations. (a) Ships must carry at least... required by § 80.1123 are properly maintained; and (6) Responsible for ensuring that the ship's navigation...

46 CFR 189.60-15 - Cargo Ship Safety Radio Certificate.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Cargo Ship Safety Radio Certificate. 189.60-15 Section..., 1974 § 189.60-15 Cargo Ship Safety Radio Certificate. Every vessel equipped with a radio installation on an international voyage must have a Cargo Ship Safety Radio Certificate. Each radio installation...

47 CFR 80.1123 - Watch requirements for ship stations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Watch requirements for ship stations. 80.1123... Procedures for Distress and Safety Communications § 80.1123 Watch requirements for ship stations. (a) While at sea, all ships must maintain a continuous watch: (1) On VHF DSC channel 70, if the ship is fitted...

46 CFR 189.60-5 - Cargo Ship Safety Construction Certificate.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Cargo Ship Safety Construction Certificate. 189.60-5..., 1974 § 189.60-5 Cargo Ship Safety Construction Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Construction Certificate. This certificate shall be issued by...

32 CFR 700.872 - Ships and craft in drydock.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 5 2011-07-01 2011-07-01 false Ships and craft in drydock. 700.872 Section 700... Special Circumstances/ships in Naval Stations and Shipyards § 700.872 Ships and craft in drydock. (a) The commanding officer of a ship in drydock shall be responsible for effecting adequate closure, during such...

33 CFR 151.29 - Foreign ships.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Foreign ships. 151.29 Section 151... Pertains to Pollution from Ships Oil Pollution § 151.29 Foreign ships. (a) Each oil tanker of 150 gross tons and above and each other ship of 400 gross tons and above, operated under the authority of a...

47 CFR 80.116 - Radiotelephone operating procedures for ship stations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Radiotelephone operating procedures for ship... Procedures-Ship Stations § 80.116 Radiotelephone operating procedures for ship stations. (a) Calling coast stations. (1) Use by ship stations of the frequency 2182 kHz for calling coast stations and for replying to...

46 CFR 153.12 - IMO Certificates for United States Ships.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false IMO Certificates for United States Ships. 153.12 Section... CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS General § 153.12 IMO Certificates for United States Ships. Either a classification society authorized under 46 CFR part...

46 CFR 131.530 - Abandon-ship training and drills.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Abandon-ship training and drills. 131.530 Section 131... OPERATIONS Tests, Drills, and Inspections § 131.530 Abandon-ship training and drills. (a) Material for abandon-ship training must be aboard each vessel. The material must consist of a manual of one or more...

33 CFR 158.240 - Ship repair yards.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Ship repair yards. 158.240... Facilities: Oily Mixtures § 158.240 Ship repair yards. The reception facility that services oceangoing ships using a ship repair yard must have a capacity for receiving— (a) An amount of ballast from bunker tanks...

46 CFR 91.60-15 - Cargo Ship Safety Radio Certificate.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Cargo Ship Safety Radio Certificate. 91.60-15 Section 91..., 1974 § 91.60-15 Cargo Ship Safety Radio Certificate. Every vessel equipped with a radio installation on an international voyage must have a Cargo Ship Safety Radio Certificate. Each radio installation must...

46 CFR 91.60-10 - Cargo Ship Safety Equipment Certificate.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Cargo Ship Safety Equipment Certificate. 91.60-10..., 1974 § 91.60-10 Cargo Ship Safety Equipment Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Equipment Certificate. (b) All such vessels shall meet the applicable...

46 CFR 189.60-15 - Cargo Ship Safety Radio Certificate.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Cargo Ship Safety Radio Certificate. 189.60-15 Section..., 1974 § 189.60-15 Cargo Ship Safety Radio Certificate. Every vessel equipped with a radio installation on an international voyage must have a Cargo Ship Safety Radio Certificate. Each radio installation...

32 CFR 700.872 - Ships and craft in drydock.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 5 2013-07-01 2013-07-01 false Ships and craft in drydock. 700.872 Section 700... Special Circumstances/ships in Naval Stations and Shipyards § 700.872 Ships and craft in drydock. (a) The commanding officer of a ship in drydock shall be responsible for effecting adequate closure, during such...

46 CFR 91.60-15 - Cargo Ship Safety Radio Certificate.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Cargo Ship Safety Radio Certificate. 91.60-15 Section 91..., 1974 § 91.60-15 Cargo Ship Safety Radio Certificate. Every vessel equipped with a radio installation on an international voyage must have a Cargo Ship Safety Radio Certificate. Each radio installation must...

46 CFR 153.12 - IMO Certificates for United States Ships.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false IMO Certificates for United States Ships. 153.12 Section... CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS General § 153.12 IMO Certificates for United States Ships. Either a classification society authorized under 46 CFR part...

46 CFR 91.60-5 - Cargo Ship Safety Construction Certificate.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Cargo Ship Safety Construction Certificate. 91.60-5..., 1974 § 91.60-5 Cargo Ship Safety Construction Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Construction Certificate. This certificate shall be issued by...

46 CFR 71.75-5 - Passenger Ship Safety Certificate.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 3 2013-10-01 2013-10-01 false Passenger Ship Safety Certificate. 71.75-5 Section 71.75... Passenger Ship Safety Certificate. (a) All vessels on or certificated for an international voyage are required to have a “ SOLAS Passenger Ship Safety Certificate.” (b) All such vessels shall meet the...

33 CFR 169.215 - How must a ship transmit position reports?

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false How must a ship transmit position... SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY SHIP REPORTING SYSTEMS Transmission of Long Range Identification and Tracking Information § 169.215 How must a ship transmit position reports? A ship must transmit...

47 CFR 80.116 - Radiotelephone operating procedures for ship stations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 5 2013-10-01 2013-10-01 false Radiotelephone operating procedures for ship... Procedures-Ship Stations § 80.116 Radiotelephone operating procedures for ship stations. (a) Calling coast stations. (1) Use by ship stations of the frequency 2182 kHz for calling coast stations and for replying to...

46 CFR 91.60-10 - Cargo Ship Safety Equipment Certificate.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Cargo Ship Safety Equipment Certificate. 91.60-10..., 1974 § 91.60-10 Cargo Ship Safety Equipment Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Equipment Certificate. (b) All such vessels shall meet the applicable...

46 CFR 153.12 - IMO Certificates for United States Ships.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false IMO Certificates for United States Ships. 153.12 Section... CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS General § 153.12 IMO Certificates for United States Ships. Either a classification society authorized under 46 CFR part...

41 CFR 301-10.182 - What classes of ship accommodations are available?

Code of Federal Regulations, 2013 CFR

2013-07-01

... 41 Public Contracts and Property Management 4 2013-07-01 2012-07-01 true What classes of ship...-TRANSPORTATION EXPENSES Common Carrier Transportation Ship § 301-10.182 What classes of ship accommodations are available? Accommodations on ships vary according to deck levels. (a) Other than lowest first-class—All...

46 CFR 71.75-5 - Passenger Ship Safety Certificate.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 3 2014-10-01 2014-10-01 false Passenger Ship Safety Certificate. 71.75-5 Section 71.75... Passenger Ship Safety Certificate. (a) All vessels on or certificated for an international voyage are required to have a “ SOLAS Passenger Ship Safety Certificate.” (b) All such vessels shall meet the...

47 CFR 80.1201 - Special provisions for cable-repair ship stations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Special provisions for cable-repair ship... Radiodetermination § 80.1201 Special provisions for cable-repair ship stations. (a) A ship station may be authorized... equipment attached to the cable-marker buoy associated with the ship station must be described in the...

47 CFR 80.1201 - Special provisions for cable-repair ship stations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 5 2013-10-01 2013-10-01 false Special provisions for cable-repair ship... Radiodetermination § 80.1201 Special provisions for cable-repair ship stations. (a) A ship station may be authorized... equipment attached to the cable-marker buoy associated with the ship station must be described in the...

46 CFR 91.60-5 - Cargo Ship Safety Construction Certificate.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Cargo Ship Safety Construction Certificate. 91.60-5..., 1974 § 91.60-5 Cargo Ship Safety Construction Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Construction Certificate. This certificate shall be issued by...

32 CFR 700.872 - Ships and craft in drydock.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 5 2012-07-01 2012-07-01 false Ships and craft in drydock. 700.872 Section 700... Special Circumstances/ships in Naval Stations and Shipyards § 700.872 Ships and craft in drydock. (a) The commanding officer of a ship in drydock shall be responsible for effecting adequate closure, during such...

46 CFR 189.60-15 - Cargo Ship Safety Radio Certificate.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Cargo Ship Safety Radio Certificate. 189.60-15 Section..., 1974 § 189.60-15 Cargo Ship Safety Radio Certificate. Every vessel equipped with a radio installation on an international voyage must have a Cargo Ship Safety Radio Certificate. Each radio installation...

46 CFR 188.10-73 - Ships' stores and supplies.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Ships' stores and supplies. 188.10-73 Section 188.10-73 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS GENERAL PROVISIONS Definition of Terms Used in This Subchapter § 188.10-73 Ships' stores and supplies. This term...

41 CFR 301-10.182 - What classes of ship accommodations are available?

Code of Federal Regulations, 2014 CFR

2014-07-01

... 41 Public Contracts and Property Management 4 2014-07-01 2014-07-01 false What classes of ship...-TRANSPORTATION EXPENSES Common Carrier Transportation Ship § 301-10.182 What classes of ship accommodations are available? Accommodations on ships vary according to deck levels. (a) Other than lowest first-class—All...

33 CFR 169.130 - When are ships required to make reports?

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false When are ships required to make... SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY SHIP REPORTING SYSTEMS Establishment of Two Mandatory Ship Reporting Systems for the Protection of Northern Right Whales § 169.130 When are ships required to make...

46 CFR 189.60-10 - Cargo Ship Safety Equipment Certificate.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Cargo Ship Safety Equipment Certificate. 189.60-10..., 1974 § 189.60-10 Cargo Ship Safety Equipment Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Equipment Certificate. (b) All such vessels shall meet the...

46 CFR 189.60-5 - Cargo Ship Safety Construction Certificate.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Cargo Ship Safety Construction Certificate. 189.60-5..., 1974 § 189.60-5 Cargo Ship Safety Construction Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Construction Certificate. This certificate shall be issued by...

46 CFR 188.10-73 - Ships' stores and supplies.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Ships' stores and supplies. 188.10-73 Section 188.10-73 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS GENERAL PROVISIONS Definition of Terms Used in This Subchapter § 188.10-73 Ships' stores and supplies. This term...

46 CFR 91.60-5 - Cargo Ship Safety Construction Certificate.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Cargo Ship Safety Construction Certificate. 91.60-5..., 1974 § 91.60-5 Cargo Ship Safety Construction Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Construction Certificate. This certificate shall be issued by...

46 CFR 189.60-10 - Cargo Ship Safety Equipment Certificate.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Cargo Ship Safety Equipment Certificate. 189.60-10..., 1974 § 189.60-10 Cargo Ship Safety Equipment Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Equipment Certificate. (b) All such vessels shall meet the...

46 CFR 91.60-15 - Cargo Ship Safety Radio Certificate.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Cargo Ship Safety Radio Certificate. 91.60-15 Section 91..., 1974 § 91.60-15 Cargo Ship Safety Radio Certificate. Every vessel equipped with a radio installation on an international voyage must have a Cargo Ship Safety Radio Certificate. Each radio installation must...

33 CFR 158.240 - Ship repair yards.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Ship repair yards. 158.240... Facilities: Oily Mixtures § 158.240 Ship repair yards. The reception facility that services oceangoing ships using a ship repair yard must have a capacity for receiving— (a) An amount of ballast from bunker tanks...

46 CFR 91.60-10 - Cargo Ship Safety Equipment Certificate.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Cargo Ship Safety Equipment Certificate. 91.60-10..., 1974 § 91.60-10 Cargo Ship Safety Equipment Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Equipment Certificate. (b) All such vessels shall meet the applicable...

46 CFR 188.10-73 - Ships' stores and supplies.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Ships' stores and supplies. 188.10-73 Section 188.10-73 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS GENERAL PROVISIONS Definition of Terms Used in This Subchapter § 188.10-73 Ships' stores and supplies. This term...

7 CFR 927.8 - Ship or handle.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 8 2013-01-01 2013-01-01 false Ship or handle. 927.8 Section 927.8 Agriculture... Order Regulating Handling Definitions § 927.8 Ship or handle. Ship or handle means to sell, deliver, consign, transport or ship pears within the production area or between the production area and any point...

47 CFR 80.1073 - Radio operator requirements for ship stations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Radio operator requirements for ship stations...) General Provisions § 80.1073 Radio operator requirements for ship stations. (a) Ships must carry at least... required by § 80.1123 are properly maintained; and (6) Responsible for ensuring that the ship's navigation...

41 CFR 301-10.182 - What classes of ship accommodations are available?

Code of Federal Regulations, 2012 CFR

2012-07-01

... 41 Public Contracts and Property Management 4 2012-07-01 2012-07-01 false What classes of ship...-TRANSPORTATION EXPENSES Common Carrier Transportation Ship § 301-10.182 What classes of ship accommodations are available? Accommodations on ships vary according to deck levels. (a) Other than lowest first-class—All...

46 CFR 189.60-5 - Cargo Ship Safety Construction Certificate.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Cargo Ship Safety Construction Certificate. 189.60-5..., 1974 § 189.60-5 Cargo Ship Safety Construction Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Construction Certificate. This certificate shall be issued by...

46 CFR 153.12 - IMO Certificates for United States Ships.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false IMO Certificates for United States Ships. 153.12 Section... CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS General § 153.12 IMO Certificates for United States Ships. Either a classification society authorized under 46 CFR part...

47 CFR 80.1073 - Radio operator requirements for ship stations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Radio operator requirements for ship stations...) General Provisions § 80.1073 Radio operator requirements for ship stations. (a) Ships must carry at least... required by § 80.1123 are properly maintained; and (6) Responsible for ensuring that the ship's navigation...

7 CFR 927.8 - Ship or handle.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 8 2012-01-01 2012-01-01 false Ship or handle. 927.8 Section 927.8 Agriculture... Order Regulating Handling Definitions § 927.8 Ship or handle. Ship or handle means to sell, deliver, consign, transport or ship pears within the production area or between the production area and any point...

33 CFR 169.130 - When are ships required to make reports?

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false When are ships required to make... SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY SHIP REPORTING SYSTEMS Establishment of Two Mandatory Ship Reporting Systems for the Protection of Northern Right Whales § 169.130 When are ships required to make...

47 CFR 80.1201 - Special provisions for cable-repair ship stations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Special provisions for cable-repair ship... Radiodetermination § 80.1201 Special provisions for cable-repair ship stations. (a) A ship station may be authorized... equipment attached to the cable-marker buoy associated with the ship station must be described in the...

46 CFR 71.75-5 - Passenger Ship Safety Certificate.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 3 2012-10-01 2012-10-01 false Passenger Ship Safety Certificate. 71.75-5 Section 71.75... Passenger Ship Safety Certificate. (a) All vessels on or certificated for an international voyage are required to have a “ SOLAS Passenger Ship Safety Certificate.” (b) All such vessels shall meet the...

32 CFR 700.872 - Ships and craft in drydock.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 5 2014-07-01 2014-07-01 false Ships and craft in drydock. 700.872 Section 700... Special Circumstances/ships in Naval Stations and Shipyards § 700.872 Ships and craft in drydock. (a) The commanding officer of a ship in drydock shall be responsible for effecting adequate closure, during such...

47 CFR 80.116 - Radiotelephone operating procedures for ship stations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Radiotelephone operating procedures for ship... Procedures-Ship Stations § 80.116 Radiotelephone operating procedures for ship stations. (a) Calling coast stations. (1) Use by ship stations of the frequency 2182 kHz for calling coast stations and for replying to...

46 CFR 189.60-5 - Cargo Ship Safety Construction Certificate.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Cargo Ship Safety Construction Certificate. 189.60-5..., 1974 § 189.60-5 Cargo Ship Safety Construction Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Construction Certificate. This certificate shall be issued by...

33 CFR 158.240 - Ship repair yards.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Ship repair yards. 158.240... Facilities: Oily Mixtures § 158.240 Ship repair yards. The reception facility that services oceangoing ships using a ship repair yard must have a capacity for receiving— (a) An amount of ballast from bunker tanks...

46 CFR 189.60-15 - Cargo Ship Safety Radio Certificate.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Cargo Ship Safety Radio Certificate. 189.60-15 Section..., 1974 § 189.60-15 Cargo Ship Safety Radio Certificate. Every vessel equipped with a radio installation on an international voyage must have a Cargo Ship Safety Radio Certificate. Each radio installation...

33 CFR 169.215 - How must a ship transmit position reports?

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false How must a ship transmit position... SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY SHIP REPORTING SYSTEMS Transmission of Long Range Identification and Tracking Information § 169.215 How must a ship transmit position reports? A ship must transmit...

47 CFR 80.116 - Radiotelephone operating procedures for ship stations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Radiotelephone operating procedures for ship... Procedures-Ship Stations § 80.116 Radiotelephone operating procedures for ship stations. (a) Calling coast stations. (1) Use by ship stations of the frequency 2182 kHz for calling coast stations and for replying to...

47 CFR 80.1073 - Radio operator requirements for ship stations.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 5 2013-10-01 2013-10-01 false Radio operator requirements for ship stations...) General Provisions § 80.1073 Radio operator requirements for ship stations. (a) Ships must carry at least... required by § 80.1123 are properly maintained; and (6) Responsible for ensuring that the ship's navigation...

33 CFR 158.240 - Ship repair yards.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Ship repair yards. 158.240... Facilities: Oily Mixtures § 158.240 Ship repair yards. The reception facility that services oceangoing ships using a ship repair yard must have a capacity for receiving— (a) An amount of ballast from bunker tanks...

7 CFR 927.8 - Ship or handle.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 8 2014-01-01 2014-01-01 false Ship or handle. 927.8 Section 927.8 Agriculture... Order Regulating Handling Definitions § 927.8 Ship or handle. Ship or handle means to sell, deliver, consign, transport or ship pears within the production area or between the production area and any point...

46 CFR 189.60-10 - Cargo Ship Safety Equipment Certificate.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Cargo Ship Safety Equipment Certificate. 189.60-10..., 1974 § 189.60-10 Cargo Ship Safety Equipment Certificate. (a) All vessels on an international voyage are required to have a Cargo Ship Safety Equipment Certificate. (b) All such vessels shall meet the...

46 CFR 160.115-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 6 2014-10-01 2014-10-01 false Operating instructions and information for the ship's... Launching Appliances-Winches § 160.115-19 Operating instructions and information for the ship's training manual. (a) Each winch must have instructions and information for the ship's training manual that use the...

46 CFR 160.135-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 6 2013-10-01 2013-10-01 false Operating instructions and information for the ship's... Lifeboats (SOLAS) § 160.135-19 Operating instructions and information for the ship's training manual. (a) Each lifeboat must have instructions and information for the ship's training manual that use the...

46 CFR 160.133-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 6 2013-10-01 2013-10-01 false Operating instructions and information for the ship's... ship's training manual. (a) Each release mechanism must have instructions and information for the ship....133-5 of this subpart) to describe the location and operation of the release mechanism. (b) The...

46 CFR 160.133-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 6 2012-10-01 2012-10-01 false Operating instructions and information for the ship's... ship's training manual. (a) Each release mechanism must have instructions and information for the ship....133-5 of this subpart) to describe the location and operation of the release mechanism. (b) The...

46 CFR 160.133-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 6 2014-10-01 2014-10-01 false Operating instructions and information for the ship's... Mechanisms for Lifeboats and Rescue Boats § 160.133-19 Operating instructions and information for the ship's training manual. (a) Each release mechanism must have instructions and information for the ship's training...

46 CFR 160.132-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 6 2013-10-01 2013-10-01 false Operating instructions and information for the ship's... Launching Appliances-Davits § 160.132-19 Operating instructions and information for the ship's training manual. (a) Each davit must have instructions and information for the ship's training manual that use the...

46 CFR 160.135-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 6 2012-10-01 2012-10-01 false Operating instructions and information for the ship's... Lifeboats (SOLAS) § 160.135-19 Operating instructions and information for the ship's training manual. (a) Each lifeboat must have instructions and information for the ship's training manual that use the...

46 CFR 160.135-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 6 2014-10-01 2014-10-01 false Operating instructions and information for the ship's... Lifeboats § 160.135-19 Operating instructions and information for the ship's training manual. (a) Each lifeboat must have instructions and information for the ship's training manual that use the symbols from...

46 CFR 160.132-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 6 2012-10-01 2012-10-01 false Operating instructions and information for the ship's... Launching Appliances-Davits § 160.132-19 Operating instructions and information for the ship's training manual. (a) Each davit must have instructions and information for the ship's training manual that use the...

46 CFR 160.132-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 6 2014-10-01 2014-10-01 false Operating instructions and information for the ship's... Launching Appliances-Davits § 160.132-19 Operating instructions and information for the ship's training manual. (a) Each davit must have instructions and information for the ship's training manual that use the...

46 CFR 160.115-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 6 2012-10-01 2012-10-01 false Operating instructions and information for the ship's... Launching Appliances-Winches § 160.115-19 Operating instructions and information for the ship's training manual. (a) Each winch must have instructions and information for the ship's training manual that use the...

46 CFR 160.115-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 6 2013-10-01 2013-10-01 false Operating instructions and information for the ship's... Launching Appliances-Winches § 160.115-19 Operating instructions and information for the ship's training manual. (a) Each winch must have instructions and information for the ship's training manual that use the...

Math Model for Naval Ship Handling Trainer.

ERIC Educational Resources Information Center

Golovcsenko, Igor V.

The report describes the math model for an experimental ship handling trainer. The training task is that of a replenishment operation at sea. The model includes equations for ship dynamics of a destroyer, propeller-engine response times, ship separation, interaction effects between supply ship and destroyer, and outputs to a visual display system.…

46 CFR 35.01-10 - Shipping papers-TB/ALL.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 1 2014-10-01 2014-10-01 false Shipping papers-TB/ALL. 35.01-10 Section 35.01-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS OPERATIONS General Provisions; Special Operating Requirements § 35.01-10 Shipping papers—TB/ALL. Each loaded tank vessel shall have on board a bill...

46 CFR 35.01-10 - Shipping papers-TB/ALL.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 1 2013-10-01 2013-10-01 false Shipping papers-TB/ALL. 35.01-10 Section 35.01-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS OPERATIONS General Provisions; Special Operating Requirements § 35.01-10 Shipping papers—TB/ALL. Each loaded tank vessel shall have on board a bill...

46 CFR 35.01-10 - Shipping papers-TB/ALL.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 1 2012-10-01 2012-10-01 false Shipping papers-TB/ALL. 35.01-10 Section 35.01-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS OPERATIONS General Provisions; Special Operating Requirements § 35.01-10 Shipping papers—TB/ALL. Each loaded tank vessel shall have on board a bill...

Infection control measures on ships and in ports during the early stage of pandemic influenza A (H1N1) 2009.

PubMed

Schlaich, Clara; Gau, Bettina; Cohen, Nicole J; Kojima, Kazunobu; Marano, Nina; Menucci, Daniel

2012-01-01

Shipping companies were surveyed to evaluate the effect of public health measures during the influenza A (H1N1) pandemic of 2009 on ship and port operations. Of 31 companies that operated 960 cruise, cargo, and other ships, 32% experienced health-screening measures by port health authorities. Approximately a quarter of ports (26%) performed screening at embarkation and 77% of shipping companies changed procedures during the early stage of the pandemic. Four companies reported outbreaks of pandemic influenza A (H1N1) 2009 on ships, which were ultimately stopped through infection control practices. Public health measures did not interfere substantially with port and ship operations with the exception of some port authorities that delayed embarking and disembarking procedures in a few ships. However, in the shipping companies' experience, measures were inconsistent between port health authorities. Access to antiviral drugs and pandemic vaccine was not provided in all ports. Current guidelines on medical care, hygiene, and emergency procedures on ships need to address pandemic influenza preparedness in future revisions.

A Fully Nonlinear, Dynamically Consistent Numerical Model for Solid-Body Ship Motion. I. Ship Motion with Fixed Heading

NASA Technical Reports Server (NTRS)

Lin, Ray-Quing; Kuang, Weijia

2011-01-01

In this paper, we describe the details of our numerical model for simulating ship solidbody motion in a given environment. In this model, the fully nonlinear dynamical equations governing the time-varying solid-body ship motion under the forces arising from ship wave interactions are solved with given initial conditions. The net force and moment (torque) on the ship body are directly calculated via integration of the hydrodynamic pressure over the wetted surface and the buoyancy effect from the underwater volume of the actual ship hull with a hybrid finite-difference/finite-element method. Neither empirical nor free parametrization is introduced in this model, i.e. no a priori experimental data are needed for modelling. This model is benchmarked with many experiments of various ship hulls for heave, roll and pitch motion. In addition to the benchmark cases, numerical experiments are also carried out for strongly nonlinear ship motion with a fixed heading. These new cases demonstrate clearly the importance of nonlinearities in ship motion modelling.

The instantaneous linear motion information measurement method based on inertial sensors for ships

NASA Astrophysics Data System (ADS)

Yang, Xu; Huang, Jing; Gao, Chen; Quan, Wei; Li, Ming; Zhang, Yanshun

2018-05-01

Ship instantaneous line motion information is the important foundation for ship control, which needs to be measured accurately. For this purpose, an instantaneous line motion measurement method based on inertial sensors is put forward for ships. By introducing a half-fixed coordinate system to realize the separation between instantaneous line motion and ship master movement, the instantaneous line motion acceleration of ships can be obtained with higher accuracy. Then, the digital high-pass filter is applied to suppress the velocity error caused by the low frequency signal such as schuler period. Finally, the instantaneous linear motion displacement of ships can be measured accurately. Simulation experimental results show that the method is reliable and effective, and can realize the precise measurement of velocity and displacement of instantaneous line motion for ships.

Development of ship structure health monitoring system based on IOT technology

NASA Astrophysics Data System (ADS)

Yang, Sujun; Shi, Lei; Chen, Demin; Dong, Yuqing; Hu, Zhenyi

2017-06-01

It is very important to monitor the ship structure, because ships are affected by all kinds of wind wave and current environment factor. At the same time, internet of things (IOT) technology plays more and more important role of in the development of industrial process. In the paper, real-time online monitoring of the ship can be realized by means of IOT technology. Ship stress, vibration and dynamic parameters are measured. Meanwhile, data is transmitted to remote monitoring system through intelligent data gateway. Timely remote support can be realized for dangerous stage of ship. Safe navigation of ships is guaranteed through application of the system.

Ships going slow in reducing their NOx emissions: changes in 2005-2012 ship exhaust inferred from satellite measurements over Europe

NASA Astrophysics Data System (ADS)

Folkert Boersma, K.; Vinken, Geert C. M.; Tournadre, Jean

2015-07-01

We address the lack of temporal information on ship emissions, and report on rapid short-term variations of satellite-derived ship NOx emissions between 2005 and 2012 over European seas. Our inversion is based on OMI observed tropospheric NO2 columns and GEOS-Chem simulations. Average European ship NOx emissions increased by ˜15% from 2005 to 2008. This increase was followed by a reduction of ˜12% in 2009, a direct result of the global economic downturn in 2008-2009, and steady emissions from 2009 to 2012. Observations of ship passages through the Suez Canal and satellite altimeter derived ship densities suggests that ships in the Mediterranean Sea have reduced their speed by more than 30% since 2008. This reduction in ship speed is accompanied by a persistent 45% reduction of average, per ship NOx emission factors. Our results indicate that the practice of ‘slow steaming’, i.e. the lowering of vessel speed to reduce fuel consumption, has indeed been implemented since 2008, and can be detected from space. In spite of the implementation of slow steaming, one in seven of all NOx molecules emitted in Europe in 2012 originated from the shipping sector, up from one in nine in 2005. The growing share of the shipping contributions to the overall European NOx emissions suggests a need for the shipping sector to implement additional measures to reduce pollutant emissions at rates that are achieved by the road transport and energy producing sectors in Europe.

Navy LPD-17 Amphibious Ship Procurement: Background, Issues, and Options for Congress

DTIC Science & Technology

2008-05-06

planned 313-ship fleet calls for a 31-ship amphibious force that includes 10 LPD-17s, and the Marine Corps states that a 33-ship amphibious force that...reflects responsibilities assigned to Marine Corps forces in U.S. regional war plans . The Navy’s FY2009 30-year (FY2009-FY2038) shipbuilding plan , if...extensions for existing amphibious ships, whether all the ships in the planned complementary Maritime Prepositionning Force of the Future (MPF[F

Navy-Marine Corps Amphibious and Maritime Prepositioning Ship Programs: Background and Oversight Issues for Congress

DTIC Science & Technology

2005-05-31

building a new amphibious assault ship called LHD-8 and is also procuring new LPD-17 class amphibious ships. A total of 12 LPD-17s were originally planned ...but the FY2006-FY2011 Future Years Defense Plan (FYDP) proposes reducing that figure to nine, with the final two to be procured in FY2006 and FY2007...Three developments have caused the Navy to reconsider its plans for procuring amphibious ships, maritime prepositioning ships, and connector ships

Navy-Marine Corps Amphibious and Maritime Prepositioning Ship Programs: Background and Oversight Issues for Congress

DTIC Science & Technology

2007-06-12

up Northrop Grumman Ship Systems ( NGSS ). Table 2. FY2008-FY2013 Amphibious and MPF(F) Ship Procurement Plan (Ships fully funded in FY2006 shown for... Plan . . 13 Navy-Marine Corps Amphibious and Maritime Prepositioning Ship Programs: Background and Oversight Issues for Congress Introduction The...is whether to approve, modify, or reject the Navy’s plans for procuring amphibious and MPF(F) ships. Decisions that Congress makes on this CRS-2 1 The

Evidence for ship noise impacts on humpback whale foraging behaviour.

PubMed

Blair, Hannah B; Merchant, Nathan D; Friedlaender, Ari S; Wiley, David N; Parks, Susan E

2016-08-01

Noise from shipping activity in North Atlantic coastal waters has been steadily increasing and is an area of growing conservation concern, as it has the potential to disrupt the behaviour of marine organisms. This study examines the impacts of ship noise on bottom foraging humpback whales (Megaptera novaeangliae) in the western North Atlantic. Data were collected from 10 foraging whales using non-invasive archival tags that simultaneously recorded underwater movements and the acoustic environment at the whale. Using mixed models, we assess the effects of ship noise on seven parameters of their feeding behaviours. Independent variables included the presence or absence of ship noise and the received level of ship noise at the whale. We found significant effects on foraging, including slower descent rates and fewer side-roll feeding events per dive with increasing ship noise. During 5 of 18 ship passages, dives without side-rolls were observed. These findings indicate that humpback whales on Stellwagen Bank, an area with chronically elevated levels of shipping traffic, significantly change foraging activity when exposed to high levels of ship noise. This measureable reduction in within-dive foraging effort of individual whales could potentially lead to population-level impacts of shipping noise on baleen whale foraging success. © 2016 The Author(s).

76 FR 82027 - Shipping Coordinating Committee; Notice of Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-29

...; --Development of guidelines for use of fiber reinforced plastic (FRP) within ship structures; --Revision of... ships; --Air pollution and energy efficiency; --Reduction of GHG emissions from ships; --Consideration...

Developing an acoustic method for reducing North Atlantic right whale (Eubalaena glacialis) ship strike mortality along the United States eastern seaboard

NASA Astrophysics Data System (ADS)

Mullen, Kaitlyn Allen

North Atlantic right whales (Eubalaena glacialis ) are among the world's most endangered cetaceans. Although protected from commercial whaling since 1949, North Atlantic right whales exhibit little to no population growth. Ship strike mortality is the leading known cause of North Atlantic right whale mortality. North Atlantic right whales exhibit developed auditory systems, and vocalize in the frequency range that dominates ship acoustic signatures. With no behavioral audiogram published, current literature assumes these whales should be able to acoustically detect signals in the same frequencies they vocalize. Recorded ship acoustic signatures occur at intensities that are similar or higher to those recorded by vocalizing North Atlantic right whales. If North Atlantic right whales are capable of acoustically detecting oncoming ship, why are they susceptible to ship strike mortality? This thesis models potential acoustic impediments to North Atlantic right whale detection of oncoming ships, and concludes the presence of modeled and observed bow null effect acoustic shadow zones, located directly ahead of oncoming ships, are likely to impair the ability of North Atlantic right whales to detect and/or localize oncoming shipping traffic. This lack of detection and/or localization likely leads to a lack of ship strike avoidance, and thus contributes to the observed high rates of North Atlantic right whale ship strike mortality. I propose that North Atlantic right whale ship strike mortality reduction is possible via reducing and/or eliminating the presence of bow null effect acoustic shadow zones. This thesis develops and tests one method for bow null effect acoustic shadow zone reduction on five ships. Finally, I review current United States policy towards North Atlantic right whale ship strike mortality in an effort to determine if the bow null effect acoustic shadow zone reduction method developed is a viable method for reducing North Atlantic right whale ship strike mortality within United States waters. I recommend that future work include additional prototype modifications and testing, application for a marine mammal scientific take authorization permit to test the modified prototype on multiple mysticete species, and continued interfacing of the prototype with evolving United States North Atlantic right whale ship strike reduction policies.

The right whale mandatory ship reporting system: a retrospective.

PubMed

Silber, Gregory K; Adams, Jeffrey D; Asaro, Michael J; Cole, Timothy V N; Moore, Katie S; Ward-Geiger, Leslie I; Zoodsma, Barbara J

2015-01-01

In 1998, the United States sought and received International Maritime Organization-endorsement of two Mandatory Ship Reporting (MSR) systems designed to improve mariner awareness about averting ship collisions with the endangered North Atlantic right whale (Eubalaena glacialis). Vessel collisions are a serious threat to the right whale and the program was among the first formal attempts to reduce this threat. Under the provisions of the MSR, all ships >300 gross tons are required to report their location, speed, and destination to a shore-based station when entering two key right whale habitats: one in waters off New England and one off coastal Georgia and Florida. In return, reporting ships receive an automatically-generated message, delivered directly to the ship's bridge, that provides information about right whale vulnerability to vessel collisions and actions mariners can take to avoid collisions. The MSR has been in operation continuously from July 1999 to the present. Archived incoming reports provided a 15-plus year history of ship operations in these two locations. We analyzed a total of 26,772 incoming MSR messages logged between July 1999 and December 2013. Most ships that were required to report did so, and compliance rates were generally constant throughout the study period. Self-reported vessel speeds when entering the systems indicated that most ships travelled between 10 and 16 (range = 5-20 +) knots. Ship speeds generally decreased in 2009 to 2013 following implementation of vessel speed restrictions. The number of reports into the southern system remained relatively constant following a steady increase through 2007, but numbers in the northern system decreased annually beginning in 2008. If reporting is indicative of long-term patterns in shipping operations, it reflects noteworthy changes in marine transportation. Observed declines in ship traffic are likely attributable to the 2008-2009 economic recession, the containerized shipping industry making increased use of larger ships that made fewer trips, and diminished oil/gas US imports as previously inaccessible domestic deposits were exploited. Recent declines in shipping activity likely resulted in lowered collision risks for right whales and reduced their exposure to underwater noise from ships.

77 FR 57084 - Public Information Collections Approved by the Office of Management and Budget

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-17

... Date: August 31, 2015. Title: Section 80.59--Compulsory ship inspections and Ship Inspection... annual inspection of certain oceangoing ships for up to 30 days beyond the expiration date of a vessel's... ships and certain passenger ships at least once a year to ensure that the radio installation is in...

46 CFR 98.30-14 - Requirements for ships carrying NLSs in portable tanks.

Code of Federal Regulations, 2010 CFR

2010-10-01

... portable tank shall ensure that— (1) The ship's Certificate of Inspection is endorsed with the name of the... condition for endorsement is met. (b) To have a ship's Certificate of Inspection endorsed to allow the... 46 Shipping 4 2010-10-01 2010-10-01 false Requirements for ships carrying NLSs in portable tanks...

46 CFR 188.10-73 - Ships' stores and supplies.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Ships' stores and supplies. 188.10-73 Section 188.10-73 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS GENERAL PROVISIONS Definition of Terms Used in This Subchapter § 188.10-73 Ships' stores and supplies. This term means any article or substance which is...

46 CFR 189.60-10 - Cargo Ship Safety Equipment Certificate.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Cargo Ship Safety Equipment Certificate. 189.60-10 Section 189.60-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS INSPECTION AND CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 189.60-10 Cargo Ship Safety...

46 CFR 189.60-5 - Cargo Ship Safety Construction Certificate.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Cargo Ship Safety Construction Certificate. 189.60-5 Section 189.60-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS INSPECTION AND CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 189.60-5 Cargo Ship Safety...

46 CFR 153.976 - Transfer of packaged cargo or ship's stores.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Transfer of packaged cargo or ship's stores. 153.976 Section 153.976 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Operations Cargo Transfer Procedures § 153.976 Transfer of...

46 CFR 189.60-15 - Cargo Ship Safety Radio Certificate.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Cargo Ship Safety Radio Certificate. 189.60-15 Section 189.60-15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS INSPECTION AND CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 189.60-15 Cargo Ship Safety Radio...

46 CFR 31.40-10 - Cargo Ship Safety Equipment Certificate-T/ALL.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo Ship Safety Equipment Certificate-T/ALL. 31.40-10... CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-10 Cargo Ship... Cargo Ship Safety Equipment Certificate. (b) All such tankships shall meet the applicable requirements...

46 CFR 45.51 - Types of ships.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Types of ships. 45.51 Section 45.51 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES GREAT LAKES LOAD LINES Freeboards § 45.51 Types of ships. (a) For the purpose of this subpart, a type A vessel has— (1) No cargo ports or similar sideshell...

46 CFR 31.40-15 - Cargo Ship Safety Radio Certificate-T/ALL.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 1 2011-10-01 2011-10-01 false Cargo Ship Safety Radio Certificate-T/ALL. 31.40-15... CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-15 Cargo Ship... voyage must have a Cargo Ship Safety Radio Certificate. Each radio installation must meet the...

46 CFR 31.40-10 - Cargo Ship Safety Equipment Certificate-T/ALL.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 1 2011-10-01 2011-10-01 false Cargo Ship Safety Equipment Certificate-T/ALL. 31.40-10... CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-10 Cargo Ship... Cargo Ship Safety Equipment Certificate. (b) All such tankships shall meet the applicable requirements...

46 CFR 31.40-5 - Cargo Ship Safety Construction Certificate-T/ALL.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo Ship Safety Construction Certificate-T/ALL. 31.40... CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-5 Cargo Ship... a Cargo Ship Safety Construction Certificate. This certificate shall be issued by the U.S. Coast...

46 CFR 31.40-5 - Cargo Ship Safety Construction Certificate-T/ALL.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 1 2011-10-01 2011-10-01 false Cargo Ship Safety Construction Certificate-T/ALL. 31.40... CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-5 Cargo Ship... a Cargo Ship Safety Construction Certificate. This certificate shall be issued by the U.S. Coast...

46 CFR 45.51 - Types of ships.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Types of ships. 45.51 Section 45.51 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES GREAT LAKES LOAD LINES Freeboards § 45.51 Types of ships. (a) For the purpose of this subpart, a type A vessel has— (1) No cargo ports or similar sideshell...

Shipping, Ships and Waterways: A Marine Education Infusion Unit. Northern New England Marine Education Project.

ERIC Educational Resources Information Center

Maine Univ., Orono. Coll. of Education.

This multidisciplinary unit is designed to increase familiarity with various types of ships and purposes for different varieties of marine vessels. It seeks to increase familiarity with routes of ocean shipping and the effect of ocean conditions such as currents upon shipping route patterns. A discussion treats the uses of various navigation…

46 CFR 31.40-10 - Cargo Ship Safety Equipment Certificate-T/ALL.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 1 2013-10-01 2013-10-01 false Cargo Ship Safety Equipment Certificate-T/ALL. 31.40-10... CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-10 Cargo Ship... Cargo Ship Safety Equipment Certificate. (b) All such tankships shall meet the applicable requirements...

46 CFR 31.40-15 - Cargo Ship Safety Radio Certificate-T/ALL.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 1 2012-10-01 2012-10-01 false Cargo Ship Safety Radio Certificate-T/ALL. 31.40-15... CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-15 Cargo Ship... voyage must have a Cargo Ship Safety Radio Certificate. Each radio installation must meet the...

46 CFR 31.40-5 - Cargo Ship Safety Construction Certificate-T/ALL.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 1 2013-10-01 2013-10-01 false Cargo Ship Safety Construction Certificate-T/ALL. 31.40... CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-5 Cargo Ship... a Cargo Ship Safety Construction Certificate. This certificate shall be issued by the U.S. Coast...

46 CFR 31.40-15 - Cargo Ship Safety Radio Certificate-T/ALL.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 1 2014-10-01 2014-10-01 false Cargo Ship Safety Radio Certificate-T/ALL. 31.40-15... CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-15 Cargo Ship... voyage must have a Cargo Ship Safety Radio Certificate. Each radio installation must meet the...

46 CFR 45.51 - Types of ships.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Types of ships. 45.51 Section 45.51 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES GREAT LAKES LOAD LINES Freeboards § 45.51 Types of ships. (a) For the purpose of this subpart, a type A vessel has— (1) No cargo ports or similar sideshell...

46 CFR 31.40-15 - Cargo Ship Safety Radio Certificate-T/ALL.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 1 2013-10-01 2013-10-01 false Cargo Ship Safety Radio Certificate-T/ALL. 31.40-15... CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-15 Cargo Ship... voyage must have a Cargo Ship Safety Radio Certificate. Each radio installation must meet the...

46 CFR 31.40-10 - Cargo Ship Safety Equipment Certificate-T/ALL.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 1 2012-10-01 2012-10-01 false Cargo Ship Safety Equipment Certificate-T/ALL. 31.40-10... CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-10 Cargo Ship... Cargo Ship Safety Equipment Certificate. (b) All such tankships shall meet the applicable requirements...

46 CFR 31.40-10 - Cargo Ship Safety Equipment Certificate-T/ALL.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 1 2014-10-01 2014-10-01 false Cargo Ship Safety Equipment Certificate-T/ALL. 31.40-10... CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-10 Cargo Ship... Cargo Ship Safety Equipment Certificate. (b) All such tankships shall meet the applicable requirements...

46 CFR 31.40-5 - Cargo Ship Safety Construction Certificate-T/ALL.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 1 2014-10-01 2014-10-01 false Cargo Ship Safety Construction Certificate-T/ALL. 31.40... CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-5 Cargo Ship... a Cargo Ship Safety Construction Certificate. This certificate shall be issued by the U.S. Coast...

46 CFR 45.51 - Types of ships.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Types of ships. 45.51 Section 45.51 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES GREAT LAKES LOAD LINES Freeboards § 45.51 Types of ships. (a) For the purpose of this subpart, a type A vessel has— (1) No cargo ports or similar sideshell...

46 CFR 31.40-5 - Cargo Ship Safety Construction Certificate-T/ALL.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 1 2012-10-01 2012-10-01 false Cargo Ship Safety Construction Certificate-T/ALL. 31.40... CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-5 Cargo Ship... a Cargo Ship Safety Construction Certificate. This certificate shall be issued by the U.S. Coast...

46 CFR 45.51 - Types of ships.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Types of ships. 45.51 Section 45.51 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES GREAT LAKES LOAD LINES Freeboards § 45.51 Types of ships. (a) For the purpose of this subpart, a type A vessel has— (1) No cargo ports or similar sideshell...

41 CFR 102-117.180 - What transportation documents must I use to ship freight?

Code of Federal Regulations, 2011 CFR

2011-01-01

... documents must I use to ship freight? 102-117.180 Section 102-117.180 Public Contracts and Property... TRANSPORTATION 117-TRANSPORTATION MANAGEMENT Shipping Freight § 102-117.180 What transportation documents must I use to ship freight? To ship freight: (a) By land (domestic shipments), use a bill of lading; (b) By...

41 CFR 102-117.180 - What transportation documents must I use to ship freight?

Code of Federal Regulations, 2013 CFR

2013-07-01

... documents must I use to ship freight? 102-117.180 Section 102-117.180 Public Contracts and Property... TRANSPORTATION 117-TRANSPORTATION MANAGEMENT Shipping Freight § 102-117.180 What transportation documents must I use to ship freight? To ship freight: (a) By land (domestic shipments), use a bill of lading; (b) By...

41 CFR 102-117.180 - What transportation documents must I use to ship freight?

Code of Federal Regulations, 2010 CFR

2010-07-01

... documents must I use to ship freight? 102-117.180 Section 102-117.180 Public Contracts and Property... TRANSPORTATION 117-TRANSPORTATION MANAGEMENT Shipping Freight § 102-117.180 What transportation documents must I use to ship freight? To ship freight: (a) By land (domestic shipments), use a bill of lading; (b) By...

41 CFR 102-117.180 - What transportation documents must I use to ship freight?

Code of Federal Regulations, 2014 CFR

2014-01-01

... documents must I use to ship freight? 102-117.180 Section 102-117.180 Public Contracts and Property... TRANSPORTATION 117-TRANSPORTATION MANAGEMENT Shipping Freight § 102-117.180 What transportation documents must I use to ship freight? To ship freight: (a) By land (domestic shipments), use a bill of lading; (b) By...

41 CFR 102-117.180 - What transportation documents must I use to ship freight?

Code of Federal Regulations, 2012 CFR

2012-01-01

... documents must I use to ship freight? 102-117.180 Section 102-117.180 Public Contracts and Property... TRANSPORTATION 117-TRANSPORTATION MANAGEMENT Shipping Freight § 102-117.180 What transportation documents must I use to ship freight? To ship freight: (a) By land (domestic shipments), use a bill of lading; (b) By...

46 CFR 160.156-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 6 2014-10-01 2014-10-01 false Operating instructions and information for the ship's... Boats and Fast Rescue Boats (SOLAS) § 160.156-19 Operating instructions and information for the ship's training manual. (a) Each rescue boat must have instructions and information for the ship's training manual...

46 CFR 160.170-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 6 2013-10-01 2013-10-01 false Operating instructions and information for the ship's... the ship's training manual. (a) In order to comply with SOLAS, each release mechanism must have instructions and information for the ship's training manual that use the symbols from IMO Res. A.760(18...

46 CFR 160.170-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 6 2012-10-01 2012-10-01 false Operating instructions and information for the ship's... the ship's training manual. (a) In order to comply with SOLAS, each release mechanism must have instructions and information for the ship's training manual that use the symbols from IMO Res. A.760(18...

41 CFR 102-117.345 - Is there a requirement for me to report to GSA on my transportation activities?

Code of Federal Regulations, 2014 CFR

2014-01-01

... an electronic formatted report on the quantity shipped, locations (from and to) and cost of... weight shipped; (3) Commodities shipped; (4) HAZMAT shipped; (5) Mode used for shipment; (6) Location of items shipped (international or domestic); and (7) Domestic subdivided by East and West (Interstate 85...

41 CFR 102-117.345 - Is there a requirement for me to report to GSA on my transportation activities?

Code of Federal Regulations, 2011 CFR

2011-01-01

... an electronic formatted report on the quantity shipped, locations (from and to) and cost of... weight shipped; (3) Commodities shipped; (4) HAZMAT shipped; (5) Mode used for shipment; (6) Location of items shipped (international or domestic); and (7) Domestic subdivided by East and West (Interstate 85...

46 CFR 160.156-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 6 2012-10-01 2012-10-01 false Operating instructions and information for the ship's... Boats and Fast Rescue Boats (SOLAS) § 160.156-19 Operating instructions and information for the ship's training manual. (a) Each rescue boat must have instructions and information for the ship's training manual...

46 CFR 160.170-19 - Operating instructions and information for the ship's training manual.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 6 2014-10-01 2014-10-01 false Operating instructions and information for the ship's... the ship's training manual. (a) In order to comply with SOLAS, each release mechanism must have instructions and information for the ship's training manual that use the symbols from IMO Res. A.760(18...

41 CFR 102-117.345 - Is there a requirement for me to report to GSA on my transportation activities?

Code of Federal Regulations, 2013 CFR

2013-07-01

... an electronic formatted report on the quantity shipped, locations (from and to) and cost of... weight shipped; (3) Commodities shipped; (4) HAZMAT shipped; (5) Mode used for shipment; (6) Location of items shipped (international or domestic); and (7) Domestic subdivided by East and West (Interstate 85...

41 CFR 102-117.345 - Is there a requirement for me to report to GSA on my transportation activities?

Code of Federal Regulations, 2012 CFR

2012-01-01

... an electronic formatted report on the quantity shipped, locations (from and to) and cost of... weight shipped; (3) Commodities shipped; (4) HAZMAT shipped; (5) Mode used for shipment; (6) Location of items shipped (international or domestic); and (7) Domestic subdivided by East and West (Interstate 85...

41 CFR 102-117.345 - Is there a requirement for me to report to GSA on my transportation activities?

Code of Federal Regulations, 2010 CFR

2010-07-01

... include an electronic formatted report on the quantity shipped, locations (from and to) and cost of... weight shipped; (3) Commodities shipped; (4) HAZMAT shipped; (5) Mode used for shipment; (6) Location of items shipped (international or domestic); and (7) Domestic subdivided by East and West (Interstate 85). ...

A technique for breaking ice in the path of a ship

NASA Technical Reports Server (NTRS)

Saunders, A. R.; Hess, R. V.; Lucht, R. A. (Inventor)

1972-01-01

A technique is described for breaking ice in the path of a ship. A laser is placed on the bow of the ship with apparatus to scan the ice in the path of the ship with the laser beam. The beam cuts or shatters the ice, enabling the ship to break the ice in its path.

46 CFR 14.313 - Storage of shipping articles and of certificates of discharge.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 1 2011-10-01 2011-10-01 false Storage of shipping articles and of certificates of... Storage of shipping articles and of certificates of discharge. (a) Each shipping company shall keep all... send to the address in § 14.103(a) for storage at the Federal Records Center at Suitland, Maryland. The...

46 CFR 14.313 - Storage of shipping articles and of certificates of discharge.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Storage of shipping articles and of certificates of... Storage of shipping articles and of certificates of discharge. (a) Each shipping company shall keep all... send to the address in § 14.103(a) for storage at the Federal Records Center at Suitland, Maryland. The...

46 CFR 14.313 - Storage of shipping articles and of certificates of discharge.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 1 2012-10-01 2012-10-01 false Storage of shipping articles and of certificates of... Storage of shipping articles and of certificates of discharge. (a) Each shipping company shall keep all... send to the address in § 14.103(a) for storage at the Federal Records Center at Suitland, Maryland. The...

Seakeeping considerations in the employment of V/STOL on Naval ships

NASA Technical Reports Server (NTRS)

Olson, S. R.

1977-01-01

Compatibility of Naval ships as V/STOL support platforms and the ship motions that V/STOL aircraft must endure are discussed. A methodology which evaluates the impact of motion criteria such as the maximum ship motion allowable during V/STOL landing/launch is presented. Emphasis is given to design alternatives that reduce ship motion.

Investigation of shipping accident injury severity and mortality.

PubMed

Weng, Jinxian; Yang, Dong

2015-03-01

Shipping movements are operated in a complex and high-risk environment. Fatal shipping accidents are the nightmares of seafarers. With ten years' worldwide ship accident data, this study develops a binary logistic regression model and a zero-truncated binomial regression model to predict the probability of fatal shipping accidents and corresponding mortalities. The model results show that both the probability of fatal accidents and mortalities are greater for collision, fire/explosion, contact, grounding, sinking accidents occurred in adverse weather conditions and darkness conditions. Sinking has the largest effects on the increment of fatal accident probability and mortalities. The results also show that the bigger number of mortalities is associated with shipping accidents occurred far away from the coastal area/harbor/port. In addition, cruise ships are found to have more mortalities than non-cruise ships. The results of this study are beneficial for policy-makers in proposing efficient strategies to prevent fatal shipping accidents. Copyright © 2015 Elsevier Ltd. All rights reserved.

Study on process design of partially-balanced, hydraulically lifting vertical ship lift

NASA Astrophysics Data System (ADS)

Xin, Shen; Xiaofeng, Xu; Lu, Zhang; Bing, Zhu; Fei, Li

2017-11-01

The hub ship lift in Panjin is the first navigation structure in China for the link between the inland and open seas, which adopts a novel partially-balanced, hydraulically lifting ship lift; it can meet such requirements as fast and sharp water level change in open sea, large draft of a yacht, and launching of a ship reception chamber; its balancing weight system can effectively reduce the load of the primary lifting cylinder, and optimize the force distribution of the ship reception chamber. The paper provides an introduction to main equipment, basic principles, main features and system composition of a ship lift. The unique power system and balancing system of the completed ship lift has offered some experience for the construction of the tourism-type ship lifts with a lower lifting height.

Analysis of Real Ship Rolling Dynamics under Wave Excitement Force Composed of Sums of Cosine Functions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Y. S.; Cai, F.; Xu, W. M.

2011-09-28

The ship motion equation with a cosine wave excitement force describes the slip moments in regular waves. A new kind of wave excitement force model, with the form as sums of cosine functions was proposed to describe ship rolling in irregular waves. Ship rolling time series were obtained by solving the ship motion equation with the fourth-order-Runger-Kutta method. These rolling time series were synthetically analyzed with methods of phase-space track, power spectrum, primary component analysis, and the largest Lyapunove exponent. Simulation results show that ship rolling presents some chaotic characteristic when the wave excitement force was applied by sums ofmore » cosine functions. The result well explains the course of ship rolling's chaotic mechanism and is useful for ship hydrodynamic study.« less

Simple analytical relations for ship bow waves

NASA Astrophysics Data System (ADS)

Noblesse, Francis; Delhommeau, G.?Rard; Guilbaud, Michel; Hendrix, Dane; Yang, Chi

Simple analytical relations for the bow wave generated by a ship in steady motion are given. Specifically, simple expressions that define the height of a ship bow wave, the distance between the ship stem and the crest of the bow wave, the rise of water at the stem, and the bow wave profile, explicitly and without calculations, in terms of the ship speed, draught, and waterline entrance angle, are given. Another result is a simple criterion that predicts, also directly and without calculations, when a ship in steady motion cannot generate a steady bow wave. This unsteady-flow criterion predicts that a ship with a sufficiently fine waterline, specifically with waterline entrance angle 2, may generate a steady bow wave at any speed. However, a ship with a fuller waterline (25E) can only generate a steady bow wave if the ship speed is higher than a critical speed, defined in terms of αE by a simple relation. No alternative criterion for predicting when a ship in steady motion does not generate a steady bow wave appears to exist. A simple expression for the height of an unsteady ship bow wave is also given. In spite of their remarkable simplicity, the relations for ship bow waves obtained in the study (using only rudimentary physical and mathematical considerations) are consistent with experimental measurements for a number of hull forms having non-bulbous wedge-shaped bows with small flare angle, and with the authors' measurements and observations for a rectangular flat plate towed at a yaw angle.