Capillary Flow Experiment

NASA Image and Video Library

2014-06-19

ISS040-E-015532 (19 June 2014) --- European Space Agency astronaut Alexander Gerst, Expedition 40 flight engineer, conducts a session with the Capillary Flow Experiment (CFE-2) in the Harmony node of the International Space Station. CFE is a suite of fluid physics experiments that investigate how fluids behave in microgravity which could benefit water and fuel delivery systems on future spacecraft. Scientists designed the CFE-2 to study properties of fluids and bubbles inside containers with a specific 3-D geometry.

Capillary Flow Experiment

NASA Image and Video Library

2014-06-19

ISS040-E-015523 (19 June 2014) --- European Space Agency astronaut Alexander Gerst, Expedition 40 flight engineer, conducts a session with the Capillary Flow Experiment (CFE-2) in the Harmony node of the International Space Station. CFE is a suite of fluid physics experiments that investigate how fluids behave in microgravity which could benefit water and fuel delivery systems on future spacecraft. Scientists designed the CFE-2 to study properties of fluids and bubbles inside containers with a specific 3-D geometry.

Capillary Flow Experiment

NASA Image and Video Library

2014-06-19

ISS040-E-015543 (19 June 2014) --- European Space Agency astronaut Alexander Gerst, Expedition 40 flight engineer, conducts a session with the Capillary Flow Experiment (CFE-2) in the Harmony node of the International Space Station. CFE is a suite of fluid physics experiments that investigate how fluids behave in microgravity which could benefit water and fuel delivery systems on future spacecraft. Scientists designed the CFE-2 to study properties of fluids and bubbles inside containers with a specific 3-D geometry.

Capillary Flow Experiment

NASA Image and Video Library

2014-06-19

ISS040-E-015536 (19 June 2014) --- European Space Agency astronaut Alexander Gerst, Expedition 40 flight engineer, conducts a session with the Capillary Flow Experiment (CFE-2) in the Harmony node of the International Space Station. CFE is a suite of fluid physics experiments that investigate how fluids behave in microgravity which could benefit water and fuel delivery systems on future spacecraft. Scientists designed the CFE-2 to study properties of fluids and bubbles inside containers with a specific 3-D geometry.

Capillary test specimen, system, and methods for in-situ visualization of capillary flow and fillet formation

DOEpatents

Hall, Aaron C.; Hosking, F. Michael ,; Reece, Mark

2003-06-24

A capillary test specimen, method, and system for visualizing and quantifying capillary flow of liquids under realistic conditions, including polymer underfilling, injection molding, soldering, brazing, and casting. The capillary test specimen simulates complex joint geometries and has an open cross-section to permit easy visual access from the side. A high-speed, high-magnification camera system records the location and shape of the moving liquid front in real-time, in-situ as it flows out of a source cavity, through an open capillary channel between two surfaces having a controlled capillary gap, and into an open fillet cavity, where it subsequently forms a fillet on free surfaces that have been configured to simulate realistic joint geometries. Electric resistance heating rapidly heats the test specimen, without using a furnace. Image-processing software analyzes the recorded images and calculates the velocity of the moving liquid front, fillet contact angles, and shape of the fillet's meniscus, among other parameters.

Ultrasonic microdialysis coupled with capillary electrophoresis electrochemiluminescence study the interaction between trimetazidine dihydrochloride and human serum albumin.

PubMed

Sun, Shuangjiao; Long, Chanjuan; Tao, Chunyao; Meng, Sa; Deng, Biyang

2014-12-03

The paper describes a homemade ultrasonic microdialysis device coupled with capillary electrophoresis electrochemiluminescence (CE-ECL) for studying the interaction between human serum albumin (HSA) and trimetazidine dihydrochloride (TMZ). The time required for equilibrium by ultrasonic microdialysis was 45min, which was far less than that by traditional dialysis (240min). It took 80min to achieve the required combination equilibrium by normal incubation and only 20min by ultrasonic. Compared with traditional dialysis, the use of ultrasonic microdialysis simplified experimental procedures, shortened experimental time and saved consumption of sample. A simple, sensitive and selective determination of TMZ was developed using CE-ECL and the parameters that affected ECL intensity were optimized. Under the optimized conditions, the linear range of TMZ was from 0.075 to 80μmol/L (r(2)=0.9974). The detection limit was 26nmol/L with RSD of 2.8%. The number of binding sites and binding constant were 1.54 and 15.17L/mol, respectively. Copyright © 2014 Elsevier B.V. All rights reserved.

Direct Numerical Simulation of Low Capillary Number Pore Scale Flows

NASA Astrophysics Data System (ADS)

Esmaeilzadeh, S.; Soulaine, C.; Tchelepi, H.

2017-12-01

The arrangement of void spaces and the granular structure of a porous medium determines multiple macroscopic properties of the rock such as porosity, capillary pressure, and relative permeability. Therefore, it is important to study the microscopic structure of the reservoir pores and understand the dynamics of fluid displacements through them. One approach for doing this, is direct numerical simulation of pore-scale flow that requires a robust numerical tool for prediction of fluid dynamics and a detailed understanding of the physical processes occurring at the pore-scale. In pore scale flows with a low capillary number, Eulerian multiphase methods are well-known to produce additional vorticity close to the interface. This is mainly due to discretization errors which lead to an imbalance of capillary pressure and surface tension forces that causes unphysical spurious currents. At the pore scale, these spurious currents can become significantly stronger than the average velocity in the phases, and lead to unphysical displacement of the interface. In this work, we first investigate the capability of the algebraic Volume of Fluid (VOF) method in OpenFOAM for low capillary number pore scale flow simulations. Afterward, we compare VOF results with a Coupled Level-Set Volume of Fluid (CLSVOF) method and Iso-Advector method. It has been shown that the former one reduces the VOF's unphysical spurious currents in some cases, and both are known to capture interfaces sharper than VOF. As the conclusion, we will investigate that whether the use of CLSVOF or Iso-Advector will lead to less spurious velocities and more accurate results for capillary driven pore-scale multiphase flows or not. Keywords: Pore-scale multiphase flow, Capillary driven flows, Spurious currents, OpenFOAM

Sheathless interface to match flow rate of capillary electrophoresis with electrospray mass spectrometry using regular-sized capillary.

PubMed

Yin, Yue; Li, Gongyu; Guan, Yafeng; Huang, Guangming

2016-08-01

The flow rate match has been a great challenge when coupling capillary electrophoresis (CE) with electrospray ionization mass spectrometry (ESI-MS). Conventional CE-ESI-MS interfaces used liquid sheath flow, narrowed capillary or additional pressure to meet this requirement; sacrifice of either capillary inner diameter (i.d.) or separation efficiency is often inevitable. Thus, a regular-sized capillary-based sheathless interface would be attractive for flow rate match in CE-MS. The regular-sized capillary-based CE-MS interface was achieved by coupling CE with induced electrospray ionization (iESI) which was stimulated by the fact that the iESI could both achieve flow rate down to 0.2 μL/min and retain ionization efficiency. The CE-iESI-MS interface was completed with an intact separation capillary, outside the outlet end of which a metal electrode was attached for the application of alternating current (ac) high voltage (HV). The feasibility of this CE-iESI-MS interface was demonstrated through the stable total ion chromatograms obtained by continuous CE infusion of tripropylamine with regular-sized capillaries. Tripropylamine and atenolol were separated and detected successfully in phosphate buffer solution (PBS) by CE-iESI-MS using a 50 or 75 μm i.d. capillary. Furthermore, this new interface showed a better signal-to-noise (S/N) of 3 to 7 times enhancement compared with another sheathless CE-ESI-MS interface that using one high voltage for both separation and electrospray when analyzing the mixture of tripropylamine and proline in NH4 OAc buffer. In addition, the reproducibility of this interface gave satisfactory results with relative standard deviation (RSD) in retention time in the range between 1% and 3%. The novel sheathless CE-MS interface introduced here could match conventional electroosmotic flow (EOF) with electrospray which could also preserve the separation efficiency and sensitivity of CE-MS. This newly developed CE-iESI-MS interface was also

Colloidal Asphaltene Deposition and Aggregation in Capillary Flow: Experiments and Mesoscopic Simulation

NASA Astrophysics Data System (ADS)

Boek, Edo S.; Ladva, Hemant K.; Crawshaw, John P.; Padding, Johan T.

2008-07-01

The aggregation and deposition of colloidal asphaltene in reservoir rock is a significant problem in the oil industry. To obtain a fundamental understanding of this phenomenon, we have studied the deposition and aggregation of colloidal asphaltene in capillary flow by experiment and simulation. For the simulation, we have used the stochastic rotation dynamics (SRD) method, in which the solvent hydrodynamic emerges from the collisions between the solvent particles, while the Brownian motion emerges naturally from the interactions between the colloidal asphaltene particles and the solvent. The asphaltene colloids interact through a screened Coulomb potential. We vary the well depth ɛ∝ and the flow rate v to obtain Peflow≫1 (hydrodynamic interactions dominate) and Re≪1 (Stokes flow). In the simulations, we impose a pressure drop over the capillary length and measure the corresponding solvent flow rate. We observe that the transient solvent flow rate decreases when the asphaltene particles become more "sticky". For a well depth ɛ∝ = 2kBT, a monolayer deposits on the capillary wall. With an increasing well depth, the capillary becomes totally blocked. The clogging is transient for ɛ∝ = 5kBT, but appears to be permanent for ɛ∝ = 10-20 kBT. We compare our simulation results with flow experiments in glass capillaries, where we use extracted asphaltenes in toluene, reprecipitated with n-heptane. In the experiments, the dynamics of asphaltene precipitation and deposition were monitored in a slot capillary using optical microscopy under flow conditions similar to those used in the simulation. Maintaining a constant flow rate of 5 μL min-1, we found that the pressure drop across the capillary first increased slowly, followed by a sharp increase, corresponding to a complete local blockage of the capillary. Doubling the flow rate to 10 μL min-1, we observe that the initial deposition occurs faster but the deposits are subsequently entrained by the flow. We

Blood Vessel Adaptation with Fluctuations in Capillary Flow Distribution

PubMed Central

Hu, Dan; Cai, David; Rangan, Aaditya V.

2012-01-01

Throughout the life of animals and human beings, blood vessel systems are continuously adapting their structures – the diameter of vessel lumina, the thickness of vessel walls, and the number of micro-vessels – to meet the changing metabolic demand of the tissue. The competition between an ever decreasing tendency of luminal diameters and an increasing stimulus from the wall shear stress plays a key role in the adaptation of luminal diameters. However, it has been shown in previous studies that the adaptation dynamics based only on these two effects is unstable. In this work, we propose a minimal adaptation model of vessel luminal diameters, in which we take into account the effects of metabolic flow regulation in addition to wall shear stresses and the decreasing tendency of luminal diameters. In particular, we study the role, in the adaptation process, of fluctuations in capillary flow distribution which is an important means of metabolic flow regulation. The fluctuation in the flow of a capillary group is idealized as a switch between two states, i.e., an open-state and a close-state. Using this model, we show that the adaptation of blood vessel system driven by wall shear stress can be efficiently stabilized when the open time ratio responds sensitively to capillary flows. As micro-vessel rarefaction is observed in our simulations with a uniformly decreased open time ratio of capillary flows, our results point to a possible origin of micro-vessel rarefaction, which is believed to induce hypertension. PMID:23029014

Fluctuations of wormlike micelle fluids in capillary flow

NASA Astrophysics Data System (ADS)

Salipante, Paul; Meek, Stephen; Hudson, Steven; Polymers; Complex Fluids Group Team

2017-11-01

We investigate the effect of entrance geometry on the flow stability of wormlike micelles solutions in capillary flow. These solutions exhibit strong shear thinning behavior resulting from micelle breakage and have been observed to undergo large flow rate fluctuations. We investigate these fluctuations using simultaneous measurements of flow rate and pressure drop across a capillary, and we adjust entrance geometry. With a tapered constriction, we observe large persistent fluctuations above a critical flow rate, characterized by rapid decreases in the pressure drop with corresponding increase in flow rate followed by a period of recovery where pressure increases and flow rate decreases. Flow field observations in the tapered entrance show large flow circulations. An abrupt contraction produces smaller transient fluidized jets forming upstream of the constriction and the magnitude of the fluctuations are significantly diminished. The effect of fluid properties is studied by comparing the magnitude and timescales of the fluctuations for surfactant systems with different relaxation times. The onset of fluctuations is compared to a criterion for the onset of elastic instabilities and the magnitude is compared to estimates for changes in channel resistance. NIST on a Chip.

Capillary Flow of Liquid Metals in Brazing

NASA Astrophysics Data System (ADS)

Dehsara, Mohammad

Capillary flow is driven or controlled by capillary forces, exerted at the triple line where the fluid phases meet the solid boundary. Phase field (PF) models naturally accommodate diffusive triple line motion with variable contact angle, thus allowing for the no-slip boundary condition without the stress singularities. Moreover, they are uniquely suited for modeling of topological discontinuities which often arise during capillary flows. In this study, we consider diffusive triple line motion within two PF models: the compositionally compressible (CC) and the incompressible (IC) models. We derive the IC model as a systematic approximation to the CC model, based on a suitable choice of continuum velocity field. The CC model, applied to the fluids of dissimilar mass densities, exhibits a computational instability at the triple line. The IC model perfectly represents the analytic equilibria. We develop the parameter identification procedure and show that the triple line kinetics can be well represented by the IC model's diffusive boundary condition. The IC model is first tested by benchmarking the phase-field and experimental kinetics of water, and silicone oil spreading over the glass plates in which two systems do not interact with the substrate. Then, two high-temperature physical settings involving spreading of the molten Al-Si alloy: one over a rough wetting substrate, the other over a non-wetting substrate are modeled in a T-joint structure which is a typical geometric configuration for many brazing and soldering applications. Surface roughness directly influences the spreading of the molten metal by causing break-ups of the liquid film and trapping the liquid away from the joint. In the early stages of capillary flow over non-wetting surface, the melting and flow are concurrent, so that the kinetics of wetting is strongly affected by the variations in effective viscosity of the partially molten metal. We define adequate time-dependent functions for the

Gas Flow and Ion Transfer in Heated ESI Capillary Interfaces

NASA Astrophysics Data System (ADS)

Bernier, Laurent; Pinfold, Harry; Pauly, Matthias; Rauschenbach, Stephan; Reiss, Julius

2018-02-01

Transfer capillaries are the preferred means to transport ions, generated by electrospray ionization, from ambient conditions to vacuum. During the transfer of ions through the narrow, long tubes into vacuum, substantial losses are typical. However, recently it was demonstrated that these losses can be avoided altogether. To understand the experimental observation and provide a general model for the ion transport, here, we investigate the ion transport through capillaries by numerical simulation of interacting ions. The simulation encompasses all relevant factors, such as space charge, diffusion, gas flow, and heating. Special attention is paid to the influence of the gas flow on the transmission and especially the change imposed by heating. The gas flow is modeled by a one-dimensional gas dynamics description. A large number of ions are treated as point particles in this gas flow. This allows to investigate the influence of the capillary heating on the gas flow and by this on the ion transport. The results are compared with experimental findings. [Figure not available: see fulltext.

Investigation of the capillary flow through open surface microfluidic structures

NASA Astrophysics Data System (ADS)

Taher, Ahmed; Jones, Benjamin; Fiorini, Paolo; Lagae, Liesbet

2017-02-01

The passive nature of capillary microfluidics for pumping and actuation of fluids is attractive for many applications including point of care medical diagnostics. For such applications, there is often the need to spot dried chemical reagents in the bottom of microfluidic channels after device fabrication; it is often more practical to have open surface devices (i.e., without a cover or lid). However, the dynamics of capillary driven flow in open surface devices have not been well studied for many geometries of interest. In this paper, we investigate capillary flow in an open surface microchannel with a backward facing step. An analytical model is developed to calculate the capillary pressure as the liquid-vapor interface traverses a backward facing step in an open microchannel. The developed model is validated against results from Surface Evolver liquid-vapor surface simulations and ANSYS Fluent two-phase flow simulations using the volume of fluid approach. Three different aspect ratios (inlet channel height by channel width) were studied. The analytical model shows good agreement with the simulation results from both modeling methods for all geometries. The analytical model is used to derive an expression for the critical aspect ratio (the minimum channel aspect ratio for flow to proceed across the backward facing step) as a function of contact angle.

Steady Capillary Driven Flow

NASA Technical Reports Server (NTRS)

Weislogel, Mark M.

1996-01-01

A steady capillary driven flow is developed for a liquid index in a circular tube which is partially coated with a surface modifier to produce a discontinuous wetting condition from one side of the tube to the other. The bulk flow is novel in that it is truly steady, and controlled solely by the physics associated with dynamic wetting. The influence of gravity on the flow is minimized through the use of small diameter tubes approximately O(1 mm) tested horizontally in a laboratory and larger tubes approximately O(10 mm) tested in the low gravity environment of a drop tower. Average steady velocities are predicted and compared against a large experimental data set which includes the effects of tube dimensions and fluid properties. The sensitivity of the velocity to surface cleanliness is dramatic and the advantages of experimentation in a microgravity environment are discussed.

Flow Modification Induced by Quincke Rotation in a Capillary

NASA Astrophysics Data System (ADS)

Cebers, A.; Lemaire, E.; Lobry, L.

When particles immersed in a semi-insulating liquid are submitted to a sufficiently high DC field, they can rotate spontaneously around any axis perpendicular to the field (Quincke rotation). Recently we have shown that due to Quincke effect the effective viscosity of a colloidal suspension could be reduced. When the suspension is submitted to a shear, the particles rotation is amplified by the electric torque and drives the suspending liquid. For a flow in a capillary, this effect manifests itself by an increase of the flow rate. We present the results of our experiments carried out with a rectangular cross section capillary. These results are compared with the direct determination of the apparent viscosity in a Couette flow rheometer.

Effects of anesthesia on the cerebral capillary blood flow in young and old mice

NASA Astrophysics Data System (ADS)

Moeini, Mohammad; Tabatabaei, Maryam S.; Bélanger, Samuel; Avti, Pramod; Castonguay, Alexandre; Pouliot, Philippe; Lesage, Frédéric

2015-03-01

Despite recent findings on the possible role of age-related cerebral microvasculature changes in cognition decline, previous studies of capillary blood flow in aging (using animal models) are scarce and limited to anesthetized conditions. Since anesthesia can have different effects in young and old animals, it may introduce a confounding effect in aging studies. The present study aimed to eliminate the potential confound introduced by anesthesia by measuring capillary blood flow parameters in both awake conditions and under isoflurane anesthesia. We used 2-photon laser scanning fluorescence microscopy to measure capillary diameter, red blood cell velocity and flux, hematocrit and capillary volumetric flow in individual capillaries in the barrel cortex of 6- and 24-month old C57Bl/6 mice. It was observed that microvascular properties are significantly affected by anesthesia leading to different trends in capillary blood flow parameters with aging when measured under awake or anesthetized conditions. The findings in this study suggest taking extra care in interpreting aging studies from anesthetized animals.

A multi points ultrasonic detection method for material flow of belt conveyor

NASA Astrophysics Data System (ADS)

Zhang, Li; He, Rongjun

2018-03-01

For big detection error of single point ultrasonic ranging technology used in material flow detection of belt conveyor when coal distributes unevenly or is large, a material flow detection method of belt conveyor is designed based on multi points ultrasonic counter ranging technology. The method can calculate approximate sectional area of material by locating multi points on surfaces of material and belt, in order to get material flow according to running speed of belt conveyor. The test results show that the method has smaller detection error than single point ultrasonic ranging technology under the condition of big coal with uneven distribution.

Flow Analysis of Isobutane (R-600A) Inside AN Adiabatic Capillary Tube

NASA Astrophysics Data System (ADS)

Alok, Praveen; Sahu, Debjyoti

2018-02-01

Capillary tubes are simple narrow tubes but the phase change which occurs inside the capillary tubes is complex to analyze. In the present investigation, an attempt is made to analyze the flow of Isobutane (R-600a) inside the coiled capillary tubes for different load conditions by Homogeneous Equilibrium Model. The Length and diameter of the capillary tube not only depend on the pressure and temperature of the condenser and evaporator but also on the cooling load. The present paper investigates the change in dimensions of the coil capillary tube with respect to the change in cooling load on the system for the constant condenser and evaporator conditions. ANSYS CFX (Central Florida Expressway) software is used to study the flow characteristics of the refrigerant. Appropriate helical coil is selected for this analysis.

Gas Flow in the Capillary of the Atmosphere-to-Vacuum Interface of Mass Spectrometers

NASA Astrophysics Data System (ADS)

Skoblin, Michael; Chudinov, Alexey; Soulimenkov, Ilia; Brusov, Vladimir; Kozlovskiy, Viacheslav

2017-10-01

Numerical simulations of a gas flow through a capillary being a part of mass spectrometer atmospheric interface were performed using a detailed laminar flow model. The simulated interface consisted of atmospheric and forevacuum volumes connected via a thin capillary. The pressure in the forevacuum volume where the gas was expanding after passing through the capillary was varied in the wide range from 10 to 900 mbar in order to study the volume flow rate as well as the other flow parameters as functions of the pressure drop between the atmospheric and forevacuum volumes. The capillary wall temperature was varied in the range from 24 to 150 °C. Numerical integration of the complete system of Navier-Stokes equations for a viscous compressible gas taking into account the heat transfer was performed using the standard gas dynamic simulation software package ANSYS CFX. The simulation results were compared with experimental measurements of gas flow parameters both performed using our experimental setup and taken from the literature. The simulated volume flow rates through the capillary differed no more than by 10% from the measured ones over the entire pressure and temperatures ranges. A conclusion was drawn that the detailed digital laminar model is able to quantitatively describe the measured gas flow rates through the capillaries under conditions considered. [Figure not available: see fulltext.

Gas Flow in the Capillary of the Atmosphere-to-Vacuum Interface of Mass Spectrometers.

PubMed

Skoblin, Michael; Chudinov, Alexey; Soulimenkov, Ilia; Brusov, Vladimir; Kozlovskiy, Viacheslav

2017-10-01

Numerical simulations of a gas flow through a capillary being a part of mass spectrometer atmospheric interface were performed using a detailed laminar flow model. The simulated interface consisted of atmospheric and forevacuum volumes connected via a thin capillary. The pressure in the forevacuum volume where the gas was expanding after passing through the capillary was varied in the wide range from 10 to 900 mbar in order to study the volume flow rate as well as the other flow parameters as functions of the pressure drop between the atmospheric and forevacuum volumes. The capillary wall temperature was varied in the range from 24 to 150 °C. Numerical integration of the complete system of Navier-Stokes equations for a viscous compressible gas taking into account the heat transfer was performed using the standard gas dynamic simulation software package ANSYS CFX. The simulation results were compared with experimental measurements of gas flow parameters both performed using our experimental setup and taken from the literature. The simulated volume flow rates through the capillary differed no more than by 10% from the measured ones over the entire pressure and temperatures ranges. A conclusion was drawn that the detailed digital laminar model is able to quantitatively describe the measured gas flow rates through the capillaries under conditions considered. Graphical Abstract ᅟ.

Capillary jets in normal gravity: Asymptotic stability analysis and excitation using Maxwell and ultrasonic radiation stresses

NASA Astrophysics Data System (ADS)

Lonzaga, Joel Barci

Both modulated ultrasonic radiation pressure and oscillating Maxwell stress from a voltage-modulated ring electrode are employed to excite low-frequency capillary modes of a weakly tapered liquid jet issuing from a nozzle. The capillary modes are waves formed at the surface of the liquid jet. The ultrasound is internally applied to the liquid jet waveguide and is cut off at a location resulting in a significantly enhanced oscillating radiation stress near the cutoff location. Alternatively, the thin electrode can generate a highly localized oscillating Maxwell stress on the jet surface. Experimental evidence shows that a spatially unstable mode with positive group velocity (propagating downstream from the excitation source) and a neutral mode with negative group velocity are both excited. Reflection at the nozzle boundary converts the neutral mode into an unstable one that interferes with the original unstable mode. The interference effect is observed downstream from the source using a laser-based optical extinction technique that detects the surface waves while the modulation frequency is scanned. This technique is very sensitive to small-amplitude disturbances. Existing linear, convective stability analyses on liquid jets accounting for the gravitational effect (i.e. varying radius and velocity) appear to be not applicable to non-slender, slow liquid jets considered here where the gravitational effect is found substantial at low flow rates. The multiple-scales method, asymptotic expansion and WKB approximation are used to derive a dispersion relation for the capillary wave similar to one obtained by Rayleigh but accounting for the gravitational effect. These mathematical tools aided by Langer's transformation are also used to derive a uniformly valid approximation for the acoustic wave propagation in a tapered cylindrical waveguide. The acoustic analytical approximation is validated by finite-element calculations. The jet response is modeled using a hybrid of

Synthetic Capillaries to Control Microscopic Blood Flow

NASA Astrophysics Data System (ADS)

Sarveswaran, K.; Kurz, V.; Dong, Z.; Tanaka, T.; Penny, S.; Timp, G.

2016-02-01

Capillaries pervade human physiology. The mean intercapillary distance is only about 100 μm in human tissue, which indicates the extent of nutrient diffusion. In engineered tissue the lack of capillaries, along with the associated perfusion, is problematic because it leads to hypoxic stress and necrosis. However, a capillary is not easy to engineer due to its complex cytoarchitecture. Here, it is shown that it is possible to create in vitro, in about 30 min, a tubular microenvironment with an elastic modulus and porosity consistent with human tissue that functionally mimicks a bona fide capillary using “live cell lithography”(LCL) to control the type and position of cells on a composite hydrogel scaffold. Furthermore, it is established that these constructs support the forces associated with blood flow, and produce nutrient gradients similar to those measured in vivo. With LCL, capillaries can be constructed with single cell precision—no other method for tissue engineering offers such precision. Since the time required for assembly scales with the number of cells, this method is likely to be adapted first to create minimal functional units of human tissue that constitute organs, consisting of a heterogeneous population of 100-1000 cells, organized hierarchically to express a predictable function.

Synthetic Capillaries to Control Microscopic Blood Flow.

PubMed

Sarveswaran, K; Kurz, V; Dong, Z; Tanaka, T; Penny, S; Timp, G

2016-02-24

Capillaries pervade human physiology. The mean intercapillary distance is only about 100 μm in human tissue, which indicates the extent of nutrient diffusion. In engineered tissue the lack of capillaries, along with the associated perfusion, is problematic because it leads to hypoxic stress and necrosis. However, a capillary is not easy to engineer due to its complex cytoarchitecture. Here, it is shown that it is possible to create in vitro, in about 30 min, a tubular microenvironment with an elastic modulus and porosity consistent with human tissue that functionally mimicks a bona fide capillary using "live cell lithography"(LCL) to control the type and position of cells on a composite hydrogel scaffold. Furthermore, it is established that these constructs support the forces associated with blood flow, and produce nutrient gradients similar to those measured in vivo. With LCL, capillaries can be constructed with single cell precision-no other method for tissue engineering offers such precision. Since the time required for assembly scales with the number of cells, this method is likely to be adapted first to create minimal functional units of human tissue that constitute organs, consisting of a heterogeneous population of 100-1000 cells, organized hierarchically to express a predictable function.

Drag reducing polymers improve coronary flow reserve through modulation of capillary resistance.

PubMed

Pacella, John J; Kameneva, Marina V; Villanueva, Flordeliza S

2009-01-01

We have shown that drag-reducing polymers (DRP) reduce microvascular resistance and improve myocardial perfusion during coronary stenosis. We used myocardial contrast echocardiography (MCE) and mathematical modeling to define the DRP microvascular effects. A non-flow-limiting left anterior descending (LAD) stenosis was created in 8 dogs. Intramyocardial blood volume, RBC velocity and flow in the LAD and circumflex (CX) beds were obtained from MCE at baseline, and in hyperemia, stenosis, hyperemia + stenosis, and hyperemia + stenosis + DRP. Microvascular resistances were calculated from a lumped-parameter model. During stenosis + hyperemia, LAD bed microvascular resistance increased (p<0.015), and capillary volume (p<0.002) and red cell velocity (p<0.0004) decreased relative to baseline. With DRP, during stenosis and hyperemia, LAD bed microvascular resistance decreased (p<0.04); there was an increase in capillary volume (p<0.007), RBC velocity (p<0.006), and flow (p<0.05). Decreased model-computed capillary resistance accounted for the reduction in LAD bed resistance after DRP. We conclude that DRP improve flow reserve during coronary stenosis by modulating capillary resistance. Primary modification of the rheological properties of blood to affect capillary resistance is a novel approach for the treatment of acute coronary syndromes.

Advanced Recording and Preprocessing of Physiological Signals. [data processing equipment for flow measurement of blood flow by ultrasonics

NASA Technical Reports Server (NTRS)

Bentley, P. B.

1975-01-01

The measurement of the volume flow-rate of blood in an artery or vein requires both an estimate of the flow velocity and its spatial distribution and the corresponding cross-sectional area. Transcutaneous measurements of these parameters can be performed using ultrasonic techniques that are analogous to the measurement of moving objects by use of a radar. Modern digital data recording and preprocessing methods were applied to the measurement of blood-flow velocity by means of the CW Doppler ultrasonic technique. Only the average flow velocity was measured and no distribution or size information was obtained. Evaluations of current flowmeter design and performance, ultrasonic transducer fabrication methods, and other related items are given. The main thrust was the development of effective data-handling and processing methods by application of modern digital techniques. The evaluation resulted in useful improvements in both the flowmeter instrumentation and the ultrasonic transducers. Effective digital processing algorithms that provided enhanced blood-flow measurement accuracy and sensitivity were developed. Block diagrams illustrative of the equipment setup are included.

Ultrasonic Doppler blood flow meter for extracorporeal circulation

NASA Astrophysics Data System (ADS)

Dantas, Ricardo G.; Costa, Eduardo T.; Maia, Joaquim M.; Nantes Button, Vera L. d. S.

2000-04-01

In cardiac surgeries it is frequently necessary to carry out interventions in internal heart structures, and where the blood circulation and oxygenation are made by artificial ways, out of the patient's body, in a procedure known as extracorporeal circulation (EC). During this procedure, one of the most important parameters, and that demands constant monitoring, is the blood flow. In this work, an ultrasonic pulsed Doppler blood flowmeter, to be used in an extracorporeal circulation system, was developed. It was used a 2 MHz ultrasonic transducer, measuring flows from 0 to 5 liters/min, coupled externally to the EC arterial line destined to adults perfusion (diameter of 9.53 mm). The experimental results using the developed flowmeter indicated a maximum deviation of 3.5% of full scale, while the blood flow estimator based in the rotation speed of the peristaltic pump presented deviations greater than 20% of full scale. This ultrasonic flowmeter supplies the results in a continuous and trustworthy way, and it does not present the limitations found in those flowmeters based in other transduction methods. Moreover, due to the fact of not being in contact with the blood, it is not disposable and it does not need sterilization, reducing operational costs and facilitating its use.

Continuous flow measurements using fixed ultrasonic meters

USGS Publications Warehouse

Oltmann, Rick

1993-01-01

USGS has or soon will be installing four continuous flow-monitoring stations in the delta that will use ultrasonic velocity meters (DVM). Funding for the stations has been provided by USGS, DWR, USBR, and Contra Costa Water District.

Capillary transit time heterogeneity and flow-metabolism coupling after traumatic brain injury

PubMed Central

Østergaard, Leif; Engedal, Thorbjørn S; Aamand, Rasmus; Mikkelsen, Ronni; Iversen, Nina K; Anzabi, Maryam; Næss-Schmidt, Erhard T; Drasbek, Kim R; Bay, Vibeke; Blicher, Jakob U; Tietze, Anna; Mikkelsen, Irene K; Hansen, Brian; Jespersen, Sune N; Juul, Niels; Sørensen, Jens CH; Rasmussen, Mads

2014-01-01

Most patients who die after traumatic brain injury (TBI) show evidence of ischemic brain damage. Nevertheless, it has proven difficult to demonstrate cerebral ischemia in TBI patients. After TBI, both global and localized changes in cerebral blood flow (CBF) are observed, depending on the extent of diffuse brain swelling and the size and location of contusions and hematoma. These changes vary considerably over time, with most TBI patients showing reduced CBF during the first 12 hours after injury, then hyperperfusion, and in some patients vasospasms before CBF eventually normalizes. This apparent neurovascular uncoupling has been ascribed to mitochondrial dysfunction, hindered oxygen diffusion into tissue, or microthrombosis. Capillary compression by astrocytic endfeet swelling is observed in biopsies acquired from TBI patients. In animal models, elevated intracranial pressure compresses capillaries, causing redistribution of capillary flows into patterns argued to cause functional shunting of oxygenated blood through the capillary bed. We used a biophysical model of oxygen transport in tissue to examine how capillary flow disturbances may contribute to the profound changes in CBF after TBI. The analysis suggests that elevated capillary transit time heterogeneity can cause critical reductions in oxygen availability in the absence of ‘classic' ischemia. We discuss diagnostic and therapeutic consequences of these predictions. PMID:25052556

Cooperative suction by vertical capillary array pump for controlling flow profiles of microfluidic sensor chips.

PubMed

Horiuchi, Tsutomu; Hayashi, Katsuyoshi; Seyama, Michiko; Inoue, Suzuyo; Tamechika, Emi

2012-10-18

A passive pump consisting of integrated vertical capillaries has been developed for a microfluidic chip as an useful component with an excellent flow volume and flow rate. A fluidic chip built into a passive pump was used by connecting the bottoms of all the capillaries to a top surface consisting of a thin layer channel in the microfluidic chip where the thin layer channel depth was smaller than the capillary radius. As a result the vertical capillaries drew fluid cooperatively rather than independently, thus exerting the maximum suction efficiency at every instance. This meant that a flow rate was realized that exhibited little variation and without any external power or operation. A microfluidic chip built into this passive pump had the ability to achieve a quasi-steady rather than a rapidly decreasing flow rate, which is a universal flow characteristic in an ordinary capillary.

Gas ultrasonic flow rate measurement through genetic-ant colony optimization based on the ultrasonic pulse received signal model

NASA Astrophysics Data System (ADS)

Hou, Huirang; Zheng, Dandan; Nie, Laixiao

2015-04-01

For gas ultrasonic flowmeters, the signals received by ultrasonic sensors are susceptible to noise interference. If signals are mingled with noise, a large error in flow measurement can be caused by triggering mistakenly using the traditional double-threshold method. To solve this problem, genetic-ant colony optimization (GACO) based on the ultrasonic pulse received signal model is proposed. Furthermore, in consideration of the real-time performance of the flow measurement system, the improvement of processing only the first three cycles of the received signals rather than the whole signal is proposed. Simulation results show that the GACO algorithm has the best estimation accuracy and ant-noise ability compared with the genetic algorithm, ant colony optimization, double-threshold and enveloped zero-crossing. Local convergence doesn’t appear with the GACO algorithm until -10 dB. For the GACO algorithm, the converging accuracy and converging speed and the amount of computation are further improved when using the first three cycles (called GACO-3cycles). Experimental results involving actual received signals show that the accuracy of single-gas ultrasonic flow rate measurement can reach 0.5% with GACO-3 cycles, which is better than with the double-threshold method.

Ultrasonic velocity profiling rheometry based on a widened circular Couette flow

NASA Astrophysics Data System (ADS)

Shiratori, Takahisa; Tasaka, Yuji; Oishi, Yoshihiko; Murai, Yuichi

2015-08-01

We propose a new rheometry for characterizing the rheological properties of fluids. The technique produces flow curves, which represent the relationship between the fluid shear rate and shear stress. Flow curves are obtained by measuring the circumferential velocity distribution of tested fluids in a circular Couette system, using an ultrasonic velocity profiling technique. By adopting a widened gap of concentric cylinders, a designed range of the shear rate is obtained so that velocity profile measurement along a single line directly acquires flow curves. To reduce the effect of ultrasonic noise on resultant flow curves, several fitting functions and variable transforms are examined to best approximate the velocity profile without introducing a priori rheological models. Silicone oil, polyacrylamide solution, and yogurt were used to evaluate the applicability of this technique. These substances are purposely targeted as examples of Newtonian fluids, shear thinning fluids, and opaque fluids with unknown rheological properties, respectively. We find that fourth-order Chebyshev polynomials provide the most accurate representation of flow curves in the context of model-free rheometry enabled by ultrasonic velocity profiling.

In vivo flow speed measurement of capillaries by photoacoustic correlation spectroscopy.

PubMed

Chen, Sung-Liang; Xie, Zhixing; Carson, Paul L; Wang, Xueding; Guo, L Jay

2011-10-15

We recently proposed photoacoustic correlation spectroscopy (PACS) and demonstrated a proof-of-concept experiment. Here we use the technique for in vivo flow speed measurement in capillaries in a chick embryo model. The photoacoustic microscopy system is used to render high spatial resolution and high sensitivity, enabling sufficient signals from single red blood cells. The probe beam size is calibrated by a blood-mimicking phantom. The results indicate the feasibility of using PACS to study flow speeds in capillaries.

Numerical Simulation of Unsteady Blood Flow through Capillary Networks.

PubMed

Davis, J M; Pozrikidis, C

2011-08-01

A numerical method is implemented for computing unsteady blood flow through a branching capillary network. The evolution of the discharge hematocrit along each capillary segment is computed by integrating in time a one-dimensional convection equation using a finite-difference method. The convection velocity is determined by the local and instantaneous effective capillary blood viscosity, while the tube to discharge hematocrit ratio is deduced from available correlations. Boundary conditions for the discharge hematocrit at divergent bifurcations arise from the partitioning law proposed by Klitzman and Johnson involving a dimensionless exponent, q≥1. When q=1, the cells are partitioned in proportion to the flow rate; as q tends to infinity, the cells are channeled into the branch with the highest flow rate. Simulations are performed for a tree-like, perfectly symmetric or randomly perturbed capillary network with m generations. When the tree involves more than a few generations, a supercritical Hopf bifurcation occurs at a critical value of q, yielding spontaneous self-sustained oscillations in the absence of external forcing. A phase diagram in the m-q plane is presented to establish conditions for unsteady flow, and the effect of various geometrical and physical parameters is examined. For a given network tree order, m, oscillations can be induced for a sufficiently high value of q by increasing the apparent intrinsic viscosity, decreasing the ratio of the vessel diameter from one generation to the next, or by decreasing the diameter of the terminal vessels. With other parameters fixed, oscillations are inhibited by increasing m. The results of the continuum model are in excellent agreement with the predictions of a discrete model where the motion of individual cells is followed from inlet to outlet.

Capillary channel flow experiments aboard the International Space Station

NASA Astrophysics Data System (ADS)

Conrath, M.; Canfield, P. J.; Bronowicki, P. M.; Dreyer, M. E.; Weislogel, M. M.; Grah, A.

2013-12-01

In the near-weightless environment of orbiting spacecraft capillary forces dominate interfacial flow phenomena over unearthly large length scales. In current experiments aboard the International Space Station, partially open channels are being investigated to determine critical flow rate-limiting conditions above which the free surface collapses ingesting bubbles. Without the natural passive phase separating qualities of buoyancy, such ingested bubbles can in turn wreak havoc on the fluid transport systems of spacecraft. The flow channels under investigation represent geometric families of conduits with applications to liquid propellant acquisition, thermal fluids circulation, and water processing for life support. Present and near future experiments focus on transient phenomena and conduit asymmetries allowing capillary forces to replace the role of gravity to perform passive phase separations. Terrestrial applications are noted where enhanced transport via direct liquid-gas contact is desired.

Numerical analysis of cell adhesion in capillary flow

NASA Astrophysics Data System (ADS)

Takeishi, Naoki; Imai, Yohsuke; Ishida, Shunichi; Omori, Toshihiro; Kamm, Roger; Ishikawa, Takuji

2016-11-01

Numerical simulation of cell adhesion was performed for capillaries whose diameter is comparable to or smaller than that of the cell. Despite a lot of works about leukocyte and tumor cell rolling, cell motion in capillaries has remained unclear. The solid and fluid mechanics of a cell in flow was coupled with a slip bond model of ligand-receptor interactions. When the size of a capillary was reduced, the cell always transitioned to "bullet-like" motion, with a consequent decrease in the velocity of the cell. A state diagram is obtained for various values of capillary diameter and receptor density. According to our numerical results, bullet motion enables firm adhesion of a cell to the capillary wall even for a weak ligand-receptor binding. We also quantified effects of various parameters, including the dissociation rate constant, the spring constant, and the reactive compliance on the characteristics of cell motion. Our results suggest that even under the interaction between PSGL-1 and P-selectin, which is mainly responsible for leukocyte rolling, a cell is able to show firm adhesion in a small capillary. These findings may help in understanding such phenomena as leukocyte plugging and cancer metastasis. This research was supported by JSPS KAKENHI Grant Numbers 25000008, 26107703, 14J03967. We also acknowledge support from the Tohoku University Division for International Advanced Research and Education Organization.

Unsaturated flow characterization utilizing water content data collected within the capillary fringe

USGS Publications Warehouse

Baehr, Arthur; Reilly, Timothy J.

2014-01-01

An analysis is presented to determine unsaturated zone hydraulic parameters based on detailed water content profiles, which can be readily acquired during hydrological investigations. Core samples taken through the unsaturated zone allow for the acquisition of gravimetrically determined water content data as a function of elevation at 3 inch intervals. This dense spacing of data provides several measurements of the water content within the capillary fringe, which are utilized to determine capillary pressure function parameters via least-squares calibration. The water content data collected above the capillary fringe are used to calculate dimensionless flow as a function of elevation providing a snapshot characterization of flow through the unsaturated zone. The water content at a flow stagnation point provides an in situ estimate of specific yield. In situ determinations of capillary pressure function parameters utilizing this method, together with particle-size distributions, can provide a valuable supplement to data libraries of unsaturated zone hydraulic parameters. The method is illustrated using data collected from plots within an agricultural research facility in Wisconsin.

On/off switching of capillary vessel flow controls mitochondrial and glycolysis pathways for energy production.

PubMed

Abo, Toru; Watanabe, Mayumi; Tomiyama, Chikako; Kanda, Yasuhiro

2014-07-01

Capillary vessel flow in the base of the fingernail can be observed by microscopy. This flow is switched off under some conditions, such as coldness, surprise, and anger and is switched on again under other conditions, such as warming, relaxation, and mild exercise. In other words, capillary vessels perform two functions: switching flow on and off. It is speculated that the switch-off function is necessary to direct energy production to the glycolysis pathway, while the switch-on function is necessary for the mitochondrial pathway. This is because glycolysis takes place under anaerobic conditions, while oxidative phosphorylation in the mitochondria proceeds under aerobic conditions in the body. To switch off circulation, the negative electric charges on the surface of erythrocytes and the capillary wall may be decreased by stimulation of the sympathetic nerves and secretion of steroid hormones. Negative charge usually acts as repulsive force between erythrocytes and between erythrocytes and the capillary wall. By decreasing the negative charge, erythrocytes can aggregate and also adhere to the capillary wall. These behaviors may be related to the capillary flow switch-off function. Here, it is emphasized that the capillary vessels possess not only a switch-on function but also a switch-off function for circulation. Copyright © 2014 Elsevier Ltd. All rights reserved.

Continuous flow measurements using ultrasonic velocity meters - an update

USGS Publications Warehouse

Oltmann, Rick

1995-01-01

An article in the summer 1993 Newsletter described USGS work to continously monitor tidal flows in the delta using ultrasonic velocity meters.  This article updates progress since 1993, including new installations, results of data analysis, damage during this year's high flows, and the status of each site.

Bubble deformations and segmented flows in corrugated microchannels at large capillary numbers

NASA Astrophysics Data System (ADS)

Sauzade, Martin; Cubaud, Thomas

2018-03-01

We experimentally investigate the interaction between individual bubble deformations and collective distortions of segmented flows in nonlinear microfluidic geometries. Using highly viscous carrier fluids, we study the evolution of monodisperse trains of gas bubbles from a square to a smoothly corrugated microchannel characterized with a series of extensions and constrictions along the flow path. The hysteresis in the bubble shape between accelerating and decelerating flow fields is shown to increase with the capillary number. Measurements of instantaneous bubble velocities reveal the presence of a capillary pull that produces a nonmonotonic behavior for the front velocity in accelerating flow regions. Functional relationships are developed for predicting the morphology and dynamics of viscous multiphase flow patterns at the pore scale.

Nanoscale Capillary Flows in Alumina: Testing the Limits of Classical Theory.

PubMed

Lei, Wenwen; McKenzie, David R

2016-07-21

Anodic aluminum oxide (AAO) membranes have well-formed cylindrical channels, as small as 10 nm in diameter, in a close packed hexagonal array. The channels in AAO membranes simulate very small leaks that may be present for example in an aluminum oxide device encapsulation. The 10 nm alumina channel is the smallest that has been studied to date for its moisture flow properties and provides a stringent test of classical capillary theory. We measure the rate at which moisture penetrates channels with diameters in the range of 10 to 120 nm with moist air present at 1 atm on one side and dry air at the same total pressure on the other. We extend classical theory for water leak rates at high humidities by allowing for variable meniscus curvature at the entrance and show that the extended theory explains why the flow increases greatly when capillary filling occurs and enables the contact angle to be determined. At low humidities our measurements for air-filled channels agree well with theory for the interdiffusive flow of water vapor in air. The flow rate of water-filled channels is one order of magnitude less than expected from classical capillary filling theory and is coincidentally equal to the helium flow rate, validating the use of helium leak testing for evaluating moisture flows in aluminum oxide leaks.

Student Design Challenges in Capillary Flow

NASA Technical Reports Server (NTRS)

Stocker, Dennis P.; Wollman, Andrew; Hall, Nancy R.; Weislogel, Mark; DeLombard, Richard

2016-01-01

For some grade 8-12 students, capillary flow has bridged the gap between the classroom and research facility, from normal gravity to microgravity. In the past four years, NASA and the Portland State University (PSU) have jointly challenged students to design test cells, using Computer-Aided Design (CAD), to study capillary action in microgravity as PSU has done on the International Space Station (ISS). Using the student-submitted CAD drawings, the test cells were manufactured by PSU and tested in their 2.1-second drop tower. The microgravity results were made available online for student analysis and reporting. Over 100 such experiments have been conducted, where there has been participation from 15 states plus a German school for the children of U.S. military personnel. In 2016, a related NASA challenge was held in partnership with the ASGSR, again, based on the research conducted by PSU. In this challenge, grade 9-12 students designed and built devices using capillary action to launch droplets as far as possible in NASAs 2.2 Second Drop Tower. Example results will be presented by students at this conference. The challenges engage students in ISS science and technology and can inspire them to pursue technical careers.

Generalized network modeling of capillary-dominated two-phase flow

NASA Astrophysics Data System (ADS)

Raeini, Ali Q.; Bijeljic, Branko; Blunt, Martin J.

2018-02-01

We present a generalized network model for simulating capillary-dominated two-phase flow through porous media at the pore scale. Three-dimensional images of the pore space are discretized using a generalized network—described in a companion paper [A. Q. Raeini, B. Bijeljic, and M. J. Blunt, Phys. Rev. E 96, 013312 (2017), 10.1103/PhysRevE.96.013312]—which comprises pores that are divided into smaller elements called half-throats and subsequently into corners. Half-throats define the connectivity of the network at the coarsest level, connecting each pore to half-throats of its neighboring pores from their narrower ends, while corners define the connectivity of pore crevices. The corners are discretized at different levels for accurate calculation of entry pressures, fluid volumes, and flow conductivities that are obtained using direct simulation of flow on the underlying image. This paper discusses the two-phase flow model that is used to compute the averaged flow properties of the generalized network, including relative permeability and capillary pressure. We validate the model using direct finite-volume two-phase flow simulations on synthetic geometries, and then present a comparison of the model predictions with a conventional pore-network model and experimental measurements of relative permeability in the literature.

Ultrasonic detection of solid phase mass flow ratio of pneumatic conveying fly ash

NASA Astrophysics Data System (ADS)

Duan, Guang Bin; Pan, Hong Li; Wang, Yong; Liu, Zong Ming

2014-04-01

In this paper, ultrasonic attenuation detection and weight balance are adopted to evaluate the solid mass ratio in this paper. Fly ash is transported on the up extraction fluidization pneumatic conveying workbench. In the ultrasonic test. McClements model and Bouguer-Lambert-Beer law model were applied to formulate the ultrasonic attenuation properties of gas-solid flow, which can give the solid mass ratio. While in the method of weigh balance, the averaged mass addition per second can reveal the solids mass flow ratio. By contrast these two solid phase mass ratio detection methods, we can know, the relative error is less.

Ultrasonic fluid flow measurement method and apparatus

DOEpatents

Kronberg, J.W.

1993-10-12

An apparatus for measuring the flow of a fluid in a pipe using ultrasonic waves. The apparatus comprises an ultrasonic generator, a lens for focusing the sound energy produced by the generator, and means for directing the focused energy into the side of the pipe through an opening and in a direction close to parallel to the long axis of the pipe. A cone carries the sound energy to the lens from the generator. Depending on the choice of materials, there may be a quarter-wave, acoustic impedance matching section between the generator and the cone to reduce the reflections of energy at the cone boundary. The lens material has an acoustic impedance similar to that of the cone material but a different sonic velocity so that the lens can converge the sound waves in the fluid. A transition section between the lens and the fluid helps to couple the energy to the fluid and assures it is directed as close to parallel to the fluid flow direction as possible. 3 figures.

Ultrasonic fluid flow measurement method and apparatus

DOEpatents

Kronberg, James W.

1993-01-01

An apparatus for measuring the flow of a fluid in a pipe using ultrasonic waves. The apparatus comprises an ultrasonic generator, a lens for focusing the sound energy produced by the generator, and means for directing the focused energy into the side of the pipe through an opening and in a direction close to parallel to the long axis of the pipe. A cone carries the sound energy to the lens from the generator. Depending on the choice of materials, there may be a quarter-wave, acoustic impedance matching section between the generator and the cone to reduce the reflections of energy at the cone boundary. The lens material has an acoustic impedance similar to that of the cone material but a different sonic velocity so that the lens can converge the sound waves in the fluid. A transition section between the lens and the fluid helps to couple the energy to the fluid and assures it is directed as close to parallel to the fluid flow direction as possible.

Stability of Buoyancy-Driven Gas Flow: Visualization of Coherent and Incoherent Gas Flow Patterns and Capillary Trapping

NASA Astrophysics Data System (ADS)

Geistlinger, H. W.; Samani, S.; Pohlert, M.; Jia, R.; Lazik, D.

2009-12-01

There are several mechanisms by which the CO2 can be stored: (1) In hydrodynamic trapping, the buoyant CO2 remains as a mobile fluid but is prevented from flowing back to the surface by an impermeable cap rock. (2) In solution trapping, CO2 dissolves into the brine, possibly enhanced by gravity instabilities due to the larger density of the brine-CO2 liquid mixture. (3) In mineral trapping, geochemical binding to the rock due to mineral precipitation. (4) In capillary trapping, the CO2 phase is disconnected into a coherent, mobile phase and an incoherent, immobile (trapped) phase. Recent analytical and numerical investigations [Juanes et al., 2006, 2009; Hesse et al., 2007 ] of buoyant-driven CO2-plume along a sloped aquifer are based on the following conceptual process model: (1) During the injection period, the less wetting CO2 displaces the more wetting brine in a drainage-like process. It is assumed that no capillary trapping occurs and that the CO2-network is coherent and driven both by the injection pressure and the buoyant pressure. Because of this coherence assumption a generalized Darcy-law can be used for the dynamics of the mobile, gaseous CO2-phase. (2) After injection the buoyant CO2 migrates laterally and upward, and water displaces CO2 at the trailing edge of the plume in an imbibition-like process. During this process, there are several physical mechanisms by which the water can displace the CO2 [Lenormand et al., 1983]. In addition to piston-type displacement, core-annular flow (also called: cooperative pore-body filling) may occur, i.e. the wetting phase moves along the walls and under certain conditions the CO2-core flow becomes unstable (snap-off). For water wet rocks, snap-off is the dominant mechanism [Al-Futaisi and Patzek, 2003; Valvatne and Blunt, 2004]. There seems to be consensus that the capillary trapping mechanism has a huge impact on the migration and distribution of CO2 which, in turn, affects the effectiveness of the other

A two-step method for rapid characterization of electroosmotic flows in capillary electrophoresis.

PubMed

Zhang, Wenjing; He, Muyi; Yuan, Tao; Xu, Wei

2017-12-01

The measurement of electroosmotic flow (EOF) is important in a capillary electrophoresis (CE) experiment in terms of performance optimization and stability improvement. Although several methods exist, there are demanding needs to accurately characterize ultra-low electroosmotic flow rates (EOF rates), such as in coated capillaries used in protein separations. In this work, a new method, called the two-step method, was developed to accurately and rapidly measure EOF rates in a capillary, especially for measuring the ultra-low EOF rates in coated capillaries. In this two-step method, the EOF rates were calculated by measuring the migration time difference of a neutral marker in two consecutive experiments, in which a pressure driven was introduced to accelerate the migration and the DC voltage was reversed to switch the EOF direction. Uncoated capillaries were first characterized by both this two-step method and a conventional method to confirm the validity of this new method. Then this new method was applied in the study of coated capillaries. Results show that this new method is not only fast in speed, but also better in accuracy. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Two-Dimensional Automatic Measurement for Nozzle Flow Distribution Using Improved Ultrasonic Sensor

PubMed Central

Zhai, Changyuan; Zhao, Chunjiang; Wang, Xiu; Wang, Ning; Zou, Wei; Li, Wei

2015-01-01

Spray deposition and distribution are affected by many factors, one of which is nozzle flow distribution. A two-dimensional automatic measurement system, which consisted of a conveying unit, a system control unit, an ultrasonic sensor, and a deposition collecting dish, was designed and developed. The system could precisely move an ultrasonic sensor above a pesticide deposition collecting dish to measure the nozzle flow distribution. A sensor sleeve with a PVC tube was designed for the ultrasonic sensor to limit its beam angle in order to measure the liquid level in the small troughs. System performance tests were conducted to verify the designed functions and measurement accuracy. A commercial spray nozzle was also used to measure its flow distribution. The test results showed that the relative error on volume measurement was less than 7.27% when the liquid volume was 2 mL in trough, while the error was less than 4.52% when the liquid volume was 4 mL or more. The developed system was also used to evaluate the flow distribution of a commercial nozzle. It was able to provide the shape and the spraying width of the flow distribution accurately. PMID:26501288

An Amorphous Network Model for Capillary Flow and Dispersion in a Partially Saturated Porous Medium

NASA Astrophysics Data System (ADS)

Simmons, C. S.; Rockhold, M. L.

2013-12-01

Network models of capillary flow are commonly used to represent conduction of fluids at pore scales. Typically, a flow system is described by a regular geometric lattice of interconnected tubes. Tubes constitute the pore throats, while connection junctions (nodes) are pore bodies. Such conceptualization of the geometry, however, is questionable for the pore scale, where irregularity clearly prevails, although prior published models using a regular lattice have demonstrated successful descriptions of the flow in the bulk medium. Here a network is allowed to be amorphous, and is not subject to any particular lattice structure. Few network flow models have treated partially saturated or even multiphase conditions. The research trend is toward using capillary tubes with triangular or square cross sections that have corners and always retain some fluid by capillarity when drained. In contrast, this model uses only circular capillaries, whose filled state is controlled by a capillary pressure rule for the junctions. The rule determines which capillary participate in the flow under an imposed matric potential gradient during steady flow conditions. Poiseuille's Law and Laplace equation are used to describe flow and water retention in the capillary units of the model. A modified conjugate gradient solution for steady flow that tracks which capillary in an amorphous network contribute to fluid conduction was devised for partially saturated conditions. The model thus retains the features of classical capillary models for determining hydraulic flow properties under unsaturated conditions based on distribution of non-interacting tubes, but now accounts for flow exchange at junctions. Continuity of the flow balance at every junction is solved simultaneously. The effective water retention relationship and unsaturated permeability are evaluated for an extensive enough network to represent a small bulk sample of porous medium. The model is applied for both a hypothetically

Capillary Flow Experiment in Node 2

NASA Image and Video Library

2013-06-15

Astronaut Karen Nyberg,Expedition 36 flight engineer,works on the Capillary Flow Experiment (CFE) Vane Gap-1 (VG-1) setup in the Node 2/Harmony. The CFE-2 vessel is used to observe fluid interface and critical wetting behavior in a cylindrical chamber with elliptic cross-section and an adjustable central perforated vane. The primary objective of the Vane Gap experiments is to determine equilibrium interface configurations and critical wetting conditions for interfaces between interior corners separated by a gap.

Self-separation of blood plasma from whole blood during the capillary flow in microchannel

NASA Astrophysics Data System (ADS)

Nunna, Bharath Babu; Zhuang, Shiqiang; Lee, Eon Soo

2017-11-01

Self-separation of blood plasma from whole blood in microchannels is of great importance due to the enormous range of applications in healthcare and diagnostics. Blood is a multiphase complex fluid, composed of cells suspended in blood plasma. RBCs are the suspended particles whose shape changes during the flow of blood. The primary constituents of blood are erythrocytes or red blood cells (RBCs), leukocytes or white blood cells (WBCs), thrombocytes or platelets and blood plasma. The existence of RBCs in blood makes the blood a non-Newtonian fluid. The current study of separation of blood plasma from whole blood during self-driven flows in a single microchannel without bifurcation, by enhancing the capillary effects. The change in the capillary effect results in a change in contact angle which directly influences the capillary flow. The flow velocity directly influences the net force acting on the RBCs and influence the separation process. The experiments are performed on the PDMS microchannels with different contact angles by altering the surface characteristics using plasma treatment. The change in the separation length is studied during the capillary flow of blood in microchannel. Bharath Babu Nunna is a researcher in mechanical engineering and implementing the novel and innovative technologies in the biomedical devices to enhance the sensitivity of the disease diagnosis.

Ultrasonic Doppler measurement of renal artery blood flow

NASA Technical Reports Server (NTRS)

1974-01-01

Implantable pulsed Doppler ultrasonic flowmeter development has resulted in designs for application to the aortas of dogs and humans, and to human renal and coronary arteries. A figure of merit was derived for each design, indicating the degree of its precision. An H-array design for transcutaneous observation of blood flow was developed and tested in vitro. Two other simplified designs for the same purpose obviate the need to determine vessel orientation. One of these will be developed in the next time period. Techniques for intraoperative use and for implantation have had mixed success. While satisfactory on large vessels, higher ultrasonic frequencies and alteration of transducer design are required for satisfactory operation of pulsed Doppler flowmeters with small vessels.

A Capillary Flow Dynamics-Based Sensing Modality for Direct Environmental Pathogen Monitoring.

PubMed

Klug, Katherine E; Reynolds, Kelly A; Yoon, Jeong-Yeol

2018-04-20

Toward ultra-simple and field-ready biosensors, we demonstrate a novel assay transducer mechanism based on interfacial property changes and capillary flow dynamics in antibody-conjugated submicron particle suspensions. Differential capillary flow is tunable, allowing pathogen quantification as a function of flow rate through a paper-based microfluidic device. Flow models based on interfacial and rheological properties indicate a significant relationship between the flow rate and the interfacial effects caused by target-particle aggregation. This mechanism is demonstrated for assays of Escherichia coli K12 in water samples and Zika virus (ZIKV) in blood serum. These assays achieved very low limits of detection compared with other demonstrated methods (1 log CFU/mL E. coli and 20 pg/mL ZIKV whole virus) with an operating time of 30 s, showing promise for environmental and health monitoring. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Non-contact ultrasonic gas flow metering using air-coupled leaky Lamb waves.

PubMed

Fan, Zichuan; Jiang, Wentao; Wright, William M D

2018-04-23

This paper describes a completely non-contact ultrasonic method of gas flow metering using air-coupled leaky Lamb waves. To show proof of principle, a simplified representation of gas flow in a duct, comprising two separated thin isotropic plates with a gas flowing between them, has been modelled and investigated experimentally. An airborne compression wave emitted from an air-coupled capacitive ultrasonic transducer excited a leaky Lamb wave in the first plate in a non-contact manner. The leakage of this Lamb wave crossed the gas flow at an angle between the two plates as a compression wave, and excited a leaky Lamb wave in the second plate. An air-coupled capacitive ultrasonic transducer on the opposite side of this second plate then detected the airborne compression wave leakage from the second Lamb wave. As the gas flow shifted the wave field between the two plates, the point of Lamb wave excitation in the second plate was displaced in proportion to the gas flow rate. Two such measurements, in opposite directions, formed a completely non-contact contra-propagating Lamb wave flow meter, allowing measurement of the flow velocity between the plates. A COMSOL Multiphysics® model was used to visualize the wave fields, and accurately predicted the time differences that were then measured experimentally. Experiments using different Lamb wave frequencies and plate materials were also similarly verified. This entirely non-contact airborne approach to Lamb wave flow metering could be applied in place of clamp-on techniques in thin-walled ducts or pipes. Copyright © 2018 Elsevier B.V. All rights reserved.

Capillary electrophoresis: Imaging of electroosmotic and pressure driven flow profiles in fused silica capillaries

NASA Technical Reports Server (NTRS)

Williams, George O., Jr.

1996-01-01

This study is a continuation of the summer of 1994 NASA/ASEE Summer Faculty Fellowship Program. This effort is a portion of the ongoing work by the Biophysics Branch of the Marshall Space Flight Center. The work has focused recently on the separation of macromolecules using capillary electrophoresis (CE). Two primary goals were established for the effort this summer. First, we wanted to use capillary electrophoresis to study the electrohydrodynamics of a sample stream. Secondly, there was a need to develop a methodology for using CE for separation of DNA molecules of various sizes. In order to achieve these goals we needed to establish a procedure for detection of a sample plug under the influence of an electric field Detection of the sample with the microscope and image analysis system would be helpful in studying the electrohydrodynamics of this stream under load. Videotaping this process under the influence of an electric field in real time would also be useful. Imaging and photography of the sample/background electrolyte interface would be vital to this study. Finally, detection and imaging of electroosmotic flow and pressure driven flow must be accomplished.

Laser Doppler flowmetry for measurement of laminar capillary blood flow in the horse

NASA Astrophysics Data System (ADS)

Adair, Henry S., III

1998-07-01

Current methods for in vivo evaluation of digital hemodynamics in the horse include angiography, scintigraphy, Doppler ultrasound, electromagnetic flow and isolated extracorporeal pump perfused digit preparations. These techniques are either non-quantifiable, do not allow for continuous measurement, require destruction of the horse orare invasive, inducing non- physiologic variables. In vitro techniques have also been reported for the evaluation of the effects of vasoactive agents on the digital vessels. The in vitro techniques are non-physiologic and have evaluated the vasculature proximal to the coronary band. Lastly, many of these techniques require general anesthesia or euthanasia of the animal. Laser Doppler flowmetry is a non-invasive, continuous measure of capillary blood flow. Laser Doppler flowmetry has been used to measure capillary blood flow in many tissues. The principle of this method is to measure the Doppler shift, that is, the frequency change that light undergoes when reflected by moving objects, such as red blood cells. Laser Doppler flowmetry records a continuous measurement of the red cell motion in the outer layer of the tissue under study, with little or no influence on physiologic blood flow. This output value constitutes the flux of red cells and is reported as capillary perfusion units. No direct information concerning oxygen, nutrient or waste metabolite exchange in the surrounding tissue is obtained. The relationship between the flowmeter output signal and the flux of red blood cells is linear. The principles of laser Doppler flowmetry will be discussed and the technique for laminar capillary blood flow measurements will be presented.

A mean curvature model for capillary flows in asymmetric containers and conduits

NASA Astrophysics Data System (ADS)

Chen, Yongkang; Tavan, Noël; Weislogel, Mark M.

2012-08-01

Capillarity-driven flows resulting from critical geometric wetting criterion are observed to yield significant shifts of the bulk fluid from one side of the container to the other during "zero gravity" experiments. For wetting fluids, such bulk shift flows consist of advancing and receding menisci sometimes separated by secondary capillary flows such as rivulet-like flows along gaps. Here we study the mean curvature of an advancing meniscus in hopes of approximating a critical boundary condition for fluid dynamics solutions. It is found that the bulk shift flows behave as if the bulk menisci are either "connected" or "disconnected." For the connected case, an analytic method is developed to calculate the mean curvature of the advancing meniscus in an asymptotic sense. In contrast, for the disconnected case the method to calculate the mean curvature of the advancing and receding menisci uses a well-established procedure. Both disconnected and connected bulk shifts can occur as the first tier flow of more complex compound capillary flows. Preliminary comparisons between the analytic method and the results of drop tower experiments are encouraging.

Electrokinetic flow in a capillary with a charge-regulating surface polymer layer.

PubMed

Keh, Huan J; Ding, Jau M

2003-07-15

An analytical study of the steady electrokinetic flow in a long uniform capillary tube or slit is presented. The inside wall of the capillary is covered by a layer of adsorbed or covalently bound charge-regulating polymer in equilibrium with the ambient electrolyte solution. In this solvent-permeable and ion-penetrable surface polyelectrolyte layer, ionogenic functional groups and frictional segments are assumed to distribute at uniform densities. The electrical potential and space charge density distributions in the cross section of the capillary are obtained by solving the linearized Poisson-Boltzmann equation. The fluid velocity profile due to the application of an electric field and a pressure gradient through the capillary is obtained from the analytical solution of a modified Navier-Stokes/Brinkman equation. Explicit formulas for the electroosmotic velocity, the average fluid velocity and electric current density on the cross section, and the streaming potential in the capillary are also derived. The results demonstrate that the direction of the electroosmotic flow and the magnitudes of the fluid velocity and electric current density are dominated by the fixed charge density inside the surface polymer layer, which is determined by the regulation characteristics such as the dissociation equilibrium constants of the ionogenic functional groups in the surface layer and the concentration of the potential-determining ions in the bulk solution.

Sheath-flow electrochemical detection of amino acids with a copper wire electrode in capillary electrophoresis.

PubMed

Inoue, Junji; Kaneta, Takashi; Imasaka, Totaro

2012-09-01

Here, we report the detection of native amino acids using a sheath-flow electrochemical detector with a working electrode made of copper wire. A separation capillary that was inserted into a platinum tube in the detector acted as a grounded electrode for electrophoresis and as a flow channel for sheath liquid. Sheath liquid flowed outside the capillary to support the transport of the separated analytes to the working electrode for electrochemical detection. The copper wire electrode was aligned at the outlet of the capillary in a wall-jet configuration. Amino acids injected into the capillary were separated following elution from the end of the capillary and detection by the copper electrode. Three kinds of copper electrodes with different diameters-50, 125, and 300 μm-were examined to investigate the effect of the electrode diameter on sensitivity. The peak widths of the analytes were independent of the diameter of the working electrode, while the 300-μm electrode led to a decrease in the signal-to-noise ratio compared with the 50- and 125-μm electrodes, which showed no significant difference. The flow rate of the sheath liquid was also varied to optimize the detection conditions. The limits of detection for amino acids ranged from 4.4 to 27 μM under optimal conditions. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Role of mixed boundaries on flow in open capillary channels with curved air-water interfaces.

PubMed

Zheng, Wenjuan; Wang, Lian-Ping; Or, Dani; Lazouskaya, Volha; Jin, Yan

2012-09-04

Flow in unsaturated porous media or in engineered microfluidic systems is dominated by capillary and viscous forces. Consequently, flow regimes may differ markedly from conventional flows, reflecting strong interfacial influences on small bodies of flowing liquids. In this work, we visualized liquid transport patterns in open capillary channels with a range of opening sizes from 0.6 to 5.0 mm using laser scanning confocal microscopy combined with fluorescent latex particles (1.0 μm) as tracers at a mean velocity of ∼0.50 mm s(-1). The observed velocity profiles indicate limited mobility at the air-water interface. The application of the Stokes equation with mixed boundary conditions (i.e., no slip on the channel walls and partial slip or shear stress at the air-water interface) clearly illustrates the increasing importance of interfacial shear stress with decreasing channel size. Interfacial shear stress emerges from the velocity gradient from the adjoining no-slip walls to the center where flow is trapped in a region in which capillary forces dominate. In addition, the increased contribution of capillary forces (relative to viscous forces) to flow on the microscale leads to increased interfacial curvature, which, together with interfacial shear stress, affects the velocity distribution and flow pattern (e.g., reverse flow in the contact line region). We found that partial slip, rather than the commonly used stress-free condition, provided a more accurate description of the boundary condition at the confined air-water interface, reflecting the key role that surface/interface effects play in controlling flow behavior on the nanoscale and microscale.

Generalized Stability Analysis of Capillary Flow in Slender V-Grooves

NASA Astrophysics Data System (ADS)

White, Nicholas; Troian, Sandra

2017-11-01

Spontaneous capillary flow, an especially rapid process in slender open microchannels resembling V-grooves, is of significant importance to many applications requiring passive robust flow control. Many types of biomedical devices for point-of-care use in developing countries are being designed around this principle. Important fundamental work by Romero and Yost (1996) and Weislogel (1996) elucidated the behavior of Newtonian films in slender V-grooves driven to flow by the streamwise change in capillary pressure due to the change in radius of curvature of the circular arc describing the interface of wetting or non-wetting fluids. Self-similar solutions describing Washburn type dynamics were found but other solutions are possible. Here we extend the Romero and Yost model to include a variety of inlet and outlet boundary conditions and examine the transient growth and generalized stability of perturbations to steady state and self-similar flows. Although most cases examined for wetting fluids exhibit robust stability against small perturbations, some exceptions reveal unstable flow. In total, these results support decades of experimental work which has found this method of flow control to be especially reliable, robust and self-healing. The authors gratefully acknowledge financial support from the 2016 NASA/Jet Propulsion Laboratory President's and Director's Fund as well as a 2017 NASA Space Technology Research Fellowship.

Continuous-Flow Electrophoresis of DNA and Proteins in a Two-Dimensional Capillary-Well Sieve.

PubMed

Duan, Lian; Cao, Zhen; Yobas, Levent

2017-09-19

Continuous-flow electrophoresis of macromolecules is demonstrated using an integrated capillary-well sieve arranged into a two-dimensional anisotropic array on silicon. The periodic array features thousands of entropic barriers, each resulting from an abrupt interface between a 2 μm deep well (channel) and a 70 nm capillary. These entropic barriers owing to two-dimensional confinement within the capillaries are vastly steep in relation to those arising from slits featuring one-dimensional confinement. Thus, the sieving mechanisms can sustain relatively large electric field strengths over a relatively small array area. The sieve rapidly sorts anionic macromolecules, including DNA chains and proteins in native or denatured states, into distinct trajectories according to size or charge under electric field vectors orthogonally applied. The baseline separation is achieved in less than 1 min within a horizontal migration length of ∼1.5 mm. The capillaries are self-enclosed conduits in cylindrical profile featuring a uniform diameter and realized through an approach that avoids advanced patterning techniques. The approach exploits a thermal reflow of a layer of doped glass for shape transformation into cylindrical capillaries and for controllably shrinking the capillary diameter. Lastly, atomic layer deposition of alumina is introduced for the first time to fine-tune the capillary diameter as well as to neutralize the surface charge, thereby suppressing undesired electroosmotic flows.

Image-Based Modeling of Blood Flow and Oxygen Transfer in Feto-Placental Capillaries

PubMed Central

Brownbill, Paul; Janáček, Jiří; Jirkovská, Marie; Kubínová, Lucie; Chernyavsky, Igor L.; Jensen, Oliver E.

2016-01-01

During pregnancy, oxygen diffuses from maternal to fetal blood through villous trees in the placenta. In this paper, we simulate blood flow and oxygen transfer in feto-placental capillaries by converting three-dimensional representations of villous and capillary surfaces, reconstructed from confocal laser scanning microscopy, to finite-element meshes, and calculating values of vascular flow resistance and total oxygen transfer. The relationship between the total oxygen transfer rate and the pressure drop through the capillary is shown to be captured across a wide range of pressure drops by physical scaling laws and an upper bound on the oxygen transfer rate. A regression equation is introduced that can be used to estimate the oxygen transfer in a capillary using the vascular resistance. Two techniques for quantifying the effects of statistical variability, experimental uncertainty and pathological placental structure on the calculated properties are then introduced. First, scaling arguments are used to quantify the sensitivity of the model to uncertainties in the geometry and the parameters. Second, the effects of localized dilations in fetal capillaries are investigated using an idealized axisymmetric model, to quantify the possible effect of pathological placental structure on oxygen transfer. The model predicts how, for a fixed pressure drop through a capillary, oxygen transfer is maximized by an optimal width of the dilation. The results could explain the prevalence of fetal hypoxia in cases of delayed villous maturation, a pathology characterized by a lack of the vasculo-syncytial membranes often seen in conjunction with localized capillary dilations. PMID:27788214

On-demand control of microfluidic flow via capillary-tuned solenoid microvalve suction.

PubMed

Zhang, Qiang; Zhang, Peiran; Su, Yetian; Mou, Chunbo; Zhou, Teng; Yang, Menglong; Xu, Jian; Ma, Bo

2014-12-21

A simple, low-cost and on-demand microfluidic flow controlling platform was developed based on a unique capillary-tuned solenoid microvalve suction effect without any outer pressure source. The suction effect was innovatively employed as a stable and controllable driving force for the manipulation of the microfluidic system by connecting a piece of capillary between the microvalve and the microfluidic chip, which caused significant hydrodynamic resistance differences among the solenoid valve ports and changed the flowing mode inside the valve. The volume of sucked liquid could be controlled from microliters even down to picoliters either by decreasing the valve energized duration (from a maximum energized duration to the valve response time of 20 ms) or by increasing the inserted capillary length (i.e., its hydrodynamic resistance). Several important microfluidic unit operations such as cell/droplet sorting and on-demand size-controllable droplet generation have been demonstrated on the developed platform and both simulations and experiments confirmed that this platform has good controllability and stability.

First Order Kinetics Visualized by Capillary Flow and Simple Data Acquisition

ERIC Educational Resources Information Center

Festersen, Lea; Gilch, Peter; Reiffers, Anna; Mundt, Ramona

2018-01-01

First order processes are of paramount importance for chemical kinetics. In a well-established demonstration experiment, the flow of water out of a vertical glass tube through a capillary simulates a chemical first order process. Here, a digital version of this experiment for lecture hall demonstrations is presented. To this end, water flowing out…

Capillary Flow in an Interior Corner

NASA Technical Reports Server (NTRS)

Weislogel, Mark Milton

1996-01-01

The design of fluids management processes in the low-gravity environment of space requires an accurate model and description of capillarity-controlled flow in containers of irregular geometry. Here we consider the capillary rise of a fluid along an interior corner of a container following a rapid reduction in gravity. The analytical portion of the work presents an asymptotic formulation in the limit of a slender fluid column, slight surface curvature along the corner, small inertia, and low gravity. New similarity solutions are found and a list of closed form expressions is provided for flow rate and column length. In particular, it is found that the flow is proportional to t(exp 1/2) for a constant height boundary condition, t(exp 2/5) for a spreading drop, and t(exp 3/5) for constant flow. In the experimental portion of the work, measurements from a 2.2s drop tower are reported. An extensive data set, collected over a previously unexplored range of flow parameters, includes estimates of repeatability and accuracy, the role of inertia and column slenderness, and the effects of corner angle, container geometry, and fluid properties. Comprehensive comparisons are made which illustrate the applicability of the analytic results to low-g fluid systems design.

Numerical simulation of flow for viscoelastic neutrophil models in a rectangular capillary network: effects of capillary shape and cell stiffness on transit time.

PubMed

Shirai, Atsushi; Fujita, Ryo; Hayase, Toshiyuki

2007-01-01

The concentration of neutrophils in the pulmonary microvasculature is higher than in large systemic vessels. It is thought that the high concentration of neutrophils facilitates their effective recruitment to sites of inflammation. Thus, in order to understand the role of neutrophils in the immune system, it is important to clarify their flow characteristics in the pulmonary microvasculature. In a previous study, we developed a model to simulate the flow of neutrophils in a capillary network, in which the cells were modeled as spheres of a Maxwell material with a cortical tension and the capillary segments were modeled as arc-shaped constrictions in straight pipes. In the present paper, the flow of neutrophils in a simplified alveolar capillary network model is investigated for various constriction shapes and cell stiffnesses. Finally, it is shown that both the coefficient of variation of the transit time of the cells, which is the standard deviation divided by the mean transit time, and the mean transit time increase as the capillary segments become steep or tight, or when the cells become hard. The mean value of the transit time exceeds the median for all of the conditions that occur in real lungs, although the difference between them is small.

Gas-liquid two-phase flow pattern identification by ultrasonic echoes reflected from the inner wall of a pipe

NASA Astrophysics Data System (ADS)

Liang, Fachun; Zheng, Hongfeng; Yu, Hao; Sun, Yuan

2016-03-01

A novel ultrasonic pulse echo method is proposed for flow pattern identification in a horizontal pipe with gas-liquid two-phase flow. A trace of echoes reflected from the pipe’s internal wall rather than the gas-liquid interface is used for flow pattern identification. Experiments were conducted in a horizontal air-water two-phase flow loop. Two ultrasonic transducers with central frequency of 5 MHz were mounted at the top and bottom of the pipe respectively. The experimental results show that the ultrasonic reflection coefficient of the wall-gas interface is much larger than that of the wall-liquid interface due to the large difference in the acoustic impedance of gas and liquid. The stratified flow, annular flow and slug flow can be successfully recognized using the attenuation ratio of the echoes. Compared with the conventional ultrasonic echo measurement method, echoes reflected from the inner surface of a pipe wall are independent of gas-liquid interface fluctuation, sound speed, and gas and liquid superficial velocities, which makes the method presented a promising technique in field practice.

Circumventing Imprecise Geometric Information and Development of a Unified Modeling Technique for Various Flow Regimes in Capillary Tubes

NASA Astrophysics Data System (ADS)

Abbasi, Bahman

2012-11-01

Owing to their manufacturability and reliability, capillary tubes are the most common expansion devices in household refrigerators. Therefore, investigating flow properties in the capillary tubes is of immense appeal in the said business. The models to predict pressure drop in two-phase internal flows invariably rely upon highly precise geometric information. The manner in which capillary tubes are manufactured makes them highly susceptible to geometric imprecisions, which renders geometry-based models unreliable to the point of obsoleteness. Aware of the issue, manufacturers categorize capillary tubes based on Nitrogen flow rate through them. This categorization method presents an opportunity to substitute geometric details with Nitrogen flow data as the basis for customized models. The simulation tools developed by implementation of this technique have the singular advantage of being applicable across flow regimes. Thus the error-prone process of identifying compatible correlations is eliminated. Equally importantly, compressibility and chocking effects can be incorporated in the same model. The outcome is a standalone correlation that provides accurate predictions, regardless of any particular fluid or flow regime. Thereby, exploratory investigations for capillary tube design and optimization are greatly simplified. Bahman Abbasi, Ph.D., is Lead Advanced Systems Engineer at General Electric Appliances in Louisville, KY. He conducts research projects across disciplines in the household refrigeration industry.

Simulation of the Flow Through Porous Layers Composed of Converging-Diverging Capillary Fissures or Tubes

NASA Astrophysics Data System (ADS)

Walicka, A.

2018-02-01

In this paper, a porous medium is modelled by a network of converging-diverging capillaries which may be considered as fissures or tubes. This model makes it necessary to consider flows through capillary fissures or tubes. Therefore an analytical method for deriving the relationships between pressure drops, volumetric flow rates and velocities for the following fluids: Newtonian, polar, power-law, pseudoplastic (DeHaven and Sisko types) and Shulmanian, was developed. Next, considerations on the models of pore network for Newtonian and non-Newtonian fluids were presented. The models, similar to the schemes of central finite differences may provide a good basis for transforming the governing equations of a flow through the porous medium into a set of linear or quasi-linear algebraic equations. It was shown that the some coefficients in these algebraic equations depend on the kind of the capillary convergence.

Stability analysis for capillary channel flow: 1d and 3d computations

NASA Astrophysics Data System (ADS)

Grah, Aleksander; Klatte, Jörg; Dreyer, Michael E.

The subject of the presentation are numerical studies on capillary channel flow, based on results of the sounding rocket TEXUS experiments. The flow through a capillary channel is established by a gear pump at the outlet. The channel, consists of two parallel glass plates with a width of 25 mm, a gap of 10 mm and a length of 12 mm. The meniscus of a compensation tube maintains a constant system pressure. Steady and dynamic pressure effects in the system force the surfaces to bend inwards. A maximum flow rate is achieved when the free surface collapses and gas ingestion occurs at the outlet. This critical flow rate depends on the channel geometry, the flow regime and the liquid properties. The aim of the experiments is the determination of the free surface shape and to find the maximum flow rate. In order to study the unsteady liquid loop behavior, a dimensionless one-dimensional model and a corresponding three-dimensional model were developed. The one-dimensional model is based on the unsteady Bernoulli equation, the unsteady continuity equation and geometrical conditions for the surface curvature and the flow cross-section. The experimental and evaluated contour data show good agreement for a sequence of transient flow rate perturbations. In the case of steady flow at maximum flow rate, when the "choking" effect occurs, the surfaces collapse and cause gas ingestion into the channel. This effect is related to the Speed Index. At the critical flow rate the Speed Index reaches the value 1, in analogy to the Mach Number. Unsteady choking does not necessarily cause surface collapse. We show, that temporarily Speed Index values exceeding One may be achieved for a perfectly stable supercritical dynamic flow. As a supercritical criterion for the dynamic free surface stability we define a Dynamic Index considering the local capillary pressure and the convective pressure, which is a function of the local velocity. The Dynamic Index is below One for stable flow while D = 1

Velocity measurements of heterogeneous RBC flow in capillary vessels using dynamic laser speckle signal.

PubMed

Li, Chenxi; Wang, Ruikang

2017-04-01

We propose an approach to measure heterogeneous velocities of red blood cells (RBCs) in capillary vessels using full-field time-varying dynamic speckle signals. The approach utilizes a low coherent laser speckle imaging system to record the instantaneous speckle pattern, followed by an eigen-decomposition-based filtering algorithm to extract dynamic speckle signal due to the moving RBCs. The velocity of heterogeneous RBC flows is determined by cross-correlating the temporal dynamic speckle signals obtained at adjacent locations. We verify the approach by imaging mouse pinna in vivo, demonstrating its capability for full-field RBC flow mapping and quantifying flow pattern with high resolution. It is expected to investigate the dynamic action of RBCs flow in capillaries under physiological changes.

Velocity measurements of heterogeneous RBC flow in capillary vessels using dynamic laser speckle signal

PubMed Central

Li, Chenxi; Wang, Ruikang

2017-01-01

Abstract. We propose an approach to measure heterogeneous velocities of red blood cells (RBCs) in capillary vessels using full-field time-varying dynamic speckle signals. The approach utilizes a low coherent laser speckle imaging system to record the instantaneous speckle pattern, followed by an eigen-decomposition-based filtering algorithm to extract dynamic speckle signal due to the moving RBCs. The velocity of heterogeneous RBC flows is determined by cross-correlating the temporal dynamic speckle signals obtained at adjacent locations. We verify the approach by imaging mouse pinna in vivo, demonstrating its capability for full-field RBC flow mapping and quantifying flow pattern with high resolution. It is expected to investigate the dynamic action of RBCs flow in capillaries under physiological changes. PMID:28384709

Construction and evaluation of a capillary array DNA sequencer based on a micromachined sheath-flow cuvette.

PubMed

Crabtree, H J; Bay, S J; Lewis, D F; Zhang, J; Coulson, L D; Fitzpatrick, G A; Delinger, S L; Harrison, D J; Dovichi, N J

2000-04-01

A capillary array electrophoresis DNA sequencer is reported based on a micromachined sheath-flow cuvette as the detection chamber. This cuvette is equipped with a set of micromachined features that hold the capillaries in precise registration to ensure uniform spacing between the capillaries, in order to generate uniform hydrodynamic flow in the cuvette. A laser beam excites all of the samples simultaneously, and a microscope objective images fluorescence onto a set of avalanche photodiodes, which operate in the analog mode. A high-gain transimpedance amplifier is used for each photodiode, providing high duty-cycle detection of fluorescence.

Time-of-flight dependency on transducer separation distance in a reflective-path guided-wave ultrasonic flow meter at zero flow conditions.

PubMed

Aanes, Magne; Kippersund, Remi Andre; Lohne, Kjetil Daae; Frøysa, Kjell-Eivind; Lunde, Per

2017-08-01

Transit-time flow meters based on guided ultrasonic wave propagation in the pipe spool have several advantages compared to traditional inline ultrasonic flow metering. The extended interrogation field, obtained by continuous leakage from guided waves traveling in the pipe wall, increases robustness toward entrained particles or gas in the flow. In reflective-path guided-wave ultrasonic flow meters (GW-UFMs), the flow equations are derived from signals propagating solely in the pipe wall and from signals passing twice through the fluid. In addition to the time-of-flight (TOF) through the fluid, the fluid path experiences an additional time delay upon reflection at the opposite pipe wall due to specular and non-specular reflections. The present work investigates the influence of these reflections on the TOF in a reflective-path GW-UFM as a function of transducer separation distance at zero flow conditions. Two models are used to describe the signal propagation through the system: (i) a transient full-wave finite element model, and (ii) a combined plane-wave and ray-tracing model. The study shows that a range-dependent time delay is associated with the reflection of the fluid path, introducing transmitter-receiver distance dependence. Based on these results, the applicability of the flow equations derived using model (ii) is discussed.

Suppression of thermally excited capillary waves by shear flow.

PubMed

Derks, Didi; Aarts, Dirk G A L; Bonn, Daniel; Lekkerkerker, Henk N W; Imhof, Arnout

2006-07-21

We investigate the thermal fluctuations of the colloidal gas-liquid interface subjected to a shear flow parallel to the interface. Strikingly, we find that the shear strongly suppresses capillary waves, making the interface smoother. This phenomenon can be described by introducing an effective interfacial tension that increases with the shear rate. The increase of sigma(eff) is a direct consequence of the loss of interfacial entropy caused by the flow, which affects especially the slow fluctuations. This demonstrates that the interfacial tension of fluids results from an intrinsic as well as a fluctuation contribution.

Simultaneous two-photon imaging of cerebral oxygenation and capillary blood flow in atherosclerotic mice

NASA Astrophysics Data System (ADS)

Lu, Xuecong; Li, Baoqiang; Moeini, Mohammad; Lesage, Frédéric

2017-02-01

Gradual changes in brain microvasculature and cerebral capillary blood flow occurring with atherosclerosis may significantly contribute to cognition decline due to their role in brain tissue oxygenation. However, previous stud- ies of the relationship between cerebral capillary blood flow and brain tissue oxygenation are limited. This study aimed to investigate vascular and concomitant changes in brain tissue pO2 with atherosclerosis. Experiments in young healthy C57B1/6 mice (n=6 , WT), young atherosclerotic mice (n=6 , ATX Y) and old atherosclerotic mice (n=6 , ATX O) were performed imaging on the left sensory-motor cortex at resting state under urethane (1.5 g/kg) anesthesia using two-photon fluorescence microscopy. The results showed that pO2 around capillaries, correlated with red blood cell (RBC) flux, increased with atherosclerosis.

Laser-bulge based ultrasonic bonding method for fabricating multilayer thermoplastic microfluidic devices

NASA Astrophysics Data System (ADS)

Liang, Chao; Liu, Chong; Liu, Ziyang; Meng, Fanjian; Li, Jingmin

2017-11-01

Ultrasonic bonding is a commonly-used method for fabrication of thermoplastic microfluidic devices. However, due to the existence of the energy director (a convex structure to concentrate the ultrasonic energy), it is difficult to control its molten polymer flow, which may result in a small gap between the bonding interface or microchannel clogging. In this paper, we present an approach to address these issues. Firstly, the microchannels were patterned onto the PMMA sheets using hot embossing with the wire electrical discharge machined molds. Then, a small bulge, which was formed at the edge of the laser-ablated groove (LG), was generated around the microchannel using a CO2 laser ablation system. By using the bulge to concentrate the ultrasonic energy, there was no need for fabricating the complicated and customized energy director. When the bulge was melted, it was able to flow into the LG which overcame the ‘gap’ and ‘clogging’ problems. Here, two types of two-layer microfluidic devices and a five-layer micromixer were fabricated to validate its performance. Our results showed that these thermoplastic microdevices can be successfully bonded by using this method. The liquid leakage was not observed in both the capillary-driven flowing test and the pressure-driven mixing experiments. It is a potential method for bonding the thermoplastic microfluidic devices.

An experimental study of the flow of LPG as refrigerant inside an adiabatic helical coiled capillary tube in vapour compression refrigeration system

NASA Astrophysics Data System (ADS)

Punia, Sanjeev Singh; Singh, Jagdev

2015-11-01

This paper presents an experimental investigation for the flow of liquefied petroleum gas (LPG) as a refrigerant inside an adiabatic helically coiled capillary tube in vapour compression refrigeration system. The effect of various geometric parameters and operating conditions like capillary tube inner diameter, length of capillary tube, coil diameter and different inlet subcoolings on the mass flow rate of LPG through the helical coiled capillary tube geometry has been investigated. It has been established that the coil diameter significantly influences the mass flow rate of LPG through the adiabatic helical capillary tube. It has been concluded that the effect of coiling of capillary tube reduces the mass flow rate by 5-12 % as compared to those of the straight capillary tube operating under similar conditions. The data obtained from the experiments are analyzed and a dimensionless correlation has been developed. The proposed correlation predicts that more than 90 % of experimental data which is in agreement with measured data in an error band of ±10 %.

Modelling of capillary-driven flow for closed paper-based microfluidic channels

NASA Astrophysics Data System (ADS)

Songok, Joel; Toivakka, Martti

2017-06-01

Paper-based microfluidics is an emerging field focused on creating inexpensive devices, with simple fabrication methods for applications in various fields including healthcare, environmental monitoring and veterinary medicine. Understanding the flow of liquid is important in achieving consistent operation of the devices. This paper proposes capillary models to predict flow in paper-based microfluidic channels, which include a flow accelerating hydrophobic top cover. The models, which consider both non-absorbing and absorbing substrates, are in good agreement with the experimental results.

Cyclic variation of ultrasonic backscattering from porcine whole blood under pulsatile flow

NASA Astrophysics Data System (ADS)

Lin, Yu-Hong

1997-10-01

The cyclic variation of ultrasonic backscattering from blood under pulsatile flow is believed to be related to the change of aggregation state of red cells and is only observed in whole blood. This study was to investigate the phenomenon by an invasive approach which was performed by inserting a 10 MHz catheter mounted transducer into a vessel. For ultrasonic measurement from blood, the most fundamental scheme is the hematocrit dependence. The backscatter maximum location was changed as the blood was stirred or stationary, as well as under steady laminar or turbulent flows. The same trend was also observed under pulsatile flow with 10% to 50% hematocrits in this study, as the backscattering to hematocrit curves were plotted at different times during a flow cycle. When the cyclic variation at 20 beats per minute (BPM) was interpreted in time domain, the enhanced aggregation at the beginning of shearing was observed. At 20 BPM with 40% hematocrit, the amplitude of cyclic variation was reduced when the shear rate was increased and the threshold of 150 s-1 was estimated. The results showed that there was no cyclic variation at 60 BPM. The backscattering was also plotted against the mean flow velocity, which demonstrated the hysteresis loops. The ultrasonic measurements showed that the relationship between the forward and backward paths of the loops were altered as beat rate, hematocrit, and shear rate were varied. Since the pulsatile flow was very complicated, a computational fluid dynamics package, FIDAPTM, was used to compute the shear rate based on the Power Law Model for non-Newtonian fluid viscosity. The non- Newtonian index and consistency in the model were computed from the viscosity to shear rate curves at 10% to 50% hematocrits measured by a cone-plate viscometer. For in vivo measurements, small pigs were used as models. Ultrasonic backscattering measurements were performed in the arteries and veins. The effect of stenosis was also investigated at the site

Noninvasive characterization of a flowing multiphase fluid using ultrasonic interferometry

DOEpatents

Sinha, Dipen N.

2003-11-11

An apparatus for noninvasively monitoring the flow and/or the composition of a flowing liquid using ultrasound is described. The position of the resonance peaks for a fluid excited by a swept-frequency ultrasonic signal have been found to change frequency both in response to a change in composition and in response to a change in the flow velocity thereof. Additionally, the distance between successive resonance peaks does not change as a function of flow, but rather in response to a change in composition. Thus, a measurement of both parameters (resonance position and resonance spacing), once calibrated, permits the simultaneous determination of flow rate and composition using the apparatus and method of the present invention.

Noninvasive Characterization Of A Flowing Multiphase Fluid Using Ultrasonic Interferometry

DOEpatents

Sinha, Dipen N.

2005-05-10

An apparatus for noninvasively monitoring the flow and/or the composition of a flowing liquid using ultrasound is described. The position of the resonance peaks for a fluid excited by a swept-frequency ultrasonic signal have been found to change frequency both in response to a change in composition and in response to a change in the flow velocity thereof. Additionally, the distance between successive resonance peaks does not change as a function of flow, but rather in response to a change in composition. Thus, a measurement of both parameters (resonance position and resonance spacing), once calibrated, permits the simultaneous determination of flow rate and composition using the apparatus and method of the present invention.

Noninvasive characterization of a flowing multiphase fluid using ultrasonic interferometry

DOEpatents

Sinha, Dipen N [Los Alamos, NM

2007-06-12

An apparatus for noninvasively monitoring the flow and/or the composition of a flowing liquid using ultrasound is described. The position of the resonance peaks for a fluid excited by a swept-frequency ultrasonic signal have been found to change frequency both in response to a change in composition and in response to a change in the flow velocity thereof. Additionally, the distance between successive resonance peaks does not change as a function of flow, but rather in response to a change in composition. Thus, a measurement of both parameters (resonance position and resonance spacing), once calibrated, permits the simultaneous determination of flow rate and composition using the apparatus and method of the present invention.

The Capillary Flow Experiments Aboard the International Space Station: Increments 9-15

NASA Technical Reports Server (NTRS)

Jenson, Ryan M.; Weislogel, Mark M.; Tavan, Noel T.; Chen, Yongkang; Semerjian, Ben; Bunnell, Charles T.; Collicott, Steven H.; Klatte, Jorg; dreyer, Michael E.

2009-01-01

This report provides a summary of the experimental, analytical, and numerical results of the Capillary Flow Experiment (CFE) performed aboard the International Space Station (ISS). The experiments were conducted in space beginning with Increment 9 through Increment 16, beginning August 2004 and ending December 2007. Both primary and extra science experiments were conducted during 19 operations performed by 7 astronauts including: M. Fincke, W. McArthur, J. Williams, S. Williams, M. Lopez-Alegria, C. Anderson, and P. Whitson. CFE consists of 6 approximately 1 to 2 kg handheld experiment units designed to investigate a selection of capillary phenomena of fundamental and applied importance, such as large length scale contact line dynamics (CFE-Contact Line), critical wetting in discontinuous structures (CFE-Vane Gap), and capillary flows and passive phase separations in complex containers (CFE-Interior Corner Flow). Highly quantitative video from the simply performed flight experiments provide data helpful in benchmarking numerical methods, confirming theoretical models, and guiding new model development. In an extensive executive summary, a brief history of the experiment is reviewed before introducing the science investigated. A selection of experimental results and comparisons with both analytic and numerical predictions is given. The subsequent chapters provide additional details of the experimental and analytical methods developed and employed. These include current presentations of the state of the data reduction which we anticipate will continue throughout the year and culminate in several more publications. An extensive appendix is used to provide support material such as an experiment history, dissemination items to date (CFE publication, etc.), detailed design drawings, and crew procedures. Despite the simple nature of the experiments and procedures, many of the experimental results may be practically employed to enhance the design of spacecraft engineering

Numerical experiment for ultrasonic-measurement-integrated simulation of three-dimensional unsteady blood flow.

PubMed

Funamoto, Kenichi; Hayase, Toshiyuki; Saijo, Yoshifumi; Yambe, Tomoyuki

2008-08-01

Integration of ultrasonic measurement and numerical simulation is a possible way to break through limitations of existing methods for obtaining complete information on hemodynamics. We herein propose Ultrasonic-Measurement-Integrated (UMI) simulation, in which feedback signals based on the optimal estimation of errors in the velocity vector determined by measured and computed Doppler velocities at feedback points are added to the governing equations. With an eye towards practical implementation of UMI simulation with real measurement data, its efficiency for three-dimensional unsteady blood flow analysis and a method for treating low time resolution of ultrasonic measurement were investigated by a numerical experiment dealing with complicated blood flow in an aneurysm. Even when simplified boundary conditions were applied, the UMI simulation reduced the errors of velocity and pressure to 31% and 53% in the feedback domain which covered the aneurysm, respectively. Local maximum wall shear stress was estimated, showing both the proper position and the value with 1% deviance. A properly designed intermittent feedback applied only at the time when measurement data were obtained had the same computational accuracy as feedback applied at every computational time step. Hence, this feedback method is a possible solution to overcome the insufficient time resolution of ultrasonic measurement.

Study of liquid and vapor flow into a Centaur capillary device

NASA Technical Reports Server (NTRS)

Blatt, M. H.; Risberg, J. A.

1979-01-01

The following areas of liquid and vapor flow were analyzed and experimentally evaluated; 1) the refilling of capillary devices with settled liquid, and 2) vapor flow across wetted screens. These investigations resulted in: 1) the development of a versatile computer program that was successfully correlated with test data and used to predict Centaur D-1S LO2 and LH2 start basket refilling; 2) the development of a semi-empirical model that was only partially correlated with data due to difficulties in obtaining repeatable test results. Also, a comparison was made to determine the best propellant management system for the Centaur D-1S vehicle. The comparison identified the basline Centaur D-1S system (using pressurization, boost pumps and propellant settling) as the best candidate based on payload weight penalty. However, other comparison criteria and advanced mission condition were identified where pressure fed systems, thermally subcooled boost pumps and capillary devices would be selected as attractive alternatives.

The unsaturated flow in porous media with dynamic capillary pressure

NASA Astrophysics Data System (ADS)

Milišić, Josipa-Pina

2018-05-01

In this paper we consider a degenerate pseudoparabolic equation for the wetting saturation of an unsaturated two-phase flow in porous media with dynamic capillary pressure-saturation relationship where the relaxation parameter depends on the saturation. Following the approach given in [13] the existence of a weak solution is proved using Galerkin approximation and regularization techniques. A priori estimates needed for passing to the limit when the regularization parameter goes to zero are obtained by using appropriate test-functions, motivated by the fact that considered PDE allows a natural generalization of the classical Kullback entropy. Finally, a special care was given in obtaining an estimate of the mixed-derivative term by combining the information from the capillary pressure with the obtained a priori estimates on the saturation.

Experimental validation of an ultrasonic flowmeter for unsteady flows

NASA Astrophysics Data System (ADS)

Leontidis, V.; Cuvier, C.; Caignaert, G.; Dupont, P.; Roussette, O.; Fammery, S.; Nivet, P.; Dazin, A.

2018-04-01

An ultrasonic flowmeter was developed for further applications in cryogenic conditions and for measuring flow rate fluctuations in the range of 0 to 70 Hz. The prototype was installed in a flow test rig, and was validated experimentally both in steady and unsteady water flow conditions. A Coriolis flowmeter was used for the calibration under steady state conditions, whereas in the unsteady case the validation was done simultaneously against two methods: particle image velocimetry (PIV), and with pressure transducers installed flush on the wall of the pipe. The results show that the developed flowmeter and the proposed methodology can accurately measure the frequency and amplitude of unsteady fluctuations in the experimental range of 0-9 l s-1 of the mean main flow rate and 0-70 Hz of the imposed disturbances.

Water imbibition by mica pores: what happens when capillary flow is suppressed?

NASA Astrophysics Data System (ADS)

Fang, Chao; Qiao, Rui

2017-11-01

The imbibition of liquids into porous media plays a critical role in numerous applications. Most prior studies focused on imbibition driven by capillary flows. In this work, we study the imbibition of water into slit-shaped mica pores filled with pressurized methane using molecular simulations. Despite that capillary flow is suppressed by the high gas pressure, water is imbibed into the pore as monolayer liquid films. Since the classical hydrodynamic flow is not readily applicable for the monolayer water film propagating on the mica wall and the imbibition is driven by the strong affinity of water molecules to the mica walls, the observed imbibition is best taken as surface hydration. We show that the dynamics of water's imbibition front follows a simple diffusive scaling law. The effective diffusion coefficient of the imbibition front, however, is more than ten times larger than the diffusion coefficient of the water molecules in the water film adsorbed on the mica walls. Using a molecular theory originally developed for the spreading of monolayer films on solid substrates, we clarify the mechanism underlying the rapid water imbibition observed here.

MRI investigation of water-oil two phase flow in straight capillary, bifurcate channel and monolayered glass bead pack.

PubMed

Liu, Yu; Jiang, Lanlan; Zhu, Ningjun; Zhao, Yuechao; Zhang, Yi; Wang, Dayong; Yang, Mingjun; Zhao, Jiafei; Song, Yongchen

2015-09-01

The study of immiscible fluid displacement between aqueous-phase liquids and non-aqueous-phase liquids in porous media is of great importance to oil recovery, groundwater contamination, and underground pollutant migration. Moreover, the attendant viscous, capillary, and gravitational forces are essential to describing the two-phase flows. In this study, magnetic resonance imaging was used to experimentally examine the detailed effects of the viscous, capillary, and gravitational forces on water-oil flows through a vertical straight capillary, bifurcate channel, and monolayered glass-bead pack. Water flooding experiments were performed at atmospheric pressure and 37.8°C, and the evolution of the distribution and saturation of the oil as well as the characteristics of the two-phase flow were investigated and analyzed. The results showed that the flow paths, i.e., the fingers of the displacing phase, during the immiscible displacement in the porous medium were determined by the viscous, capillary, and gravitational forces as well as the sizes of the pores and throats. The experimental results afford a fundamental understanding of immiscible fluid displacement in a porous medium. Copyright © 2015 Elsevier Inc. All rights reserved.

Capillary Corner Flows With Partial and Nonwetting Fluids

NASA Technical Reports Server (NTRS)

Bolleddula, D. A.; Weislogel, M. M.

2009-01-01

Capillary flow in containers or conduits with interior corners are common place in nature and industry. The majority of investigations addressing such flows solve the problem numerically in terms of a friction factor for flows along corners with contact angles below the Concus-Finn critical wetting condition for the particular conduit geometry of interest. This research effort provides missing numerical data for the flow resistance function F(sub i) for partially and nonwetting systems above the Concus-Finn condition. In such cases the fluid spontaneously de-wets the interior corner and often retracts into corner-bound drops. A banded numerical coefficient is desirable for further analysis and is achieved by careful selection of length scales x(sub s) and y(sub s) to nondimensionalize the problem. The optimal scaling is found to be identical to the wetting scaling, namely x(sub s) = H and y(sub s) = Htan (alpha), where H is the height from the corner to the free surface and a is the corner half-angle. Employing this scaling produces a relatively weakly varying flow resistance F(sub i) and for subsequent analyses is treated as a constant. Example solutions to steady and transient flow problems are provided that illustrate applications of this result.

A model and numerical method for compressible flows with capillary effects

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schmidmayer, Kevin, E-mail: kevin.schmidmayer@univ-amu.fr; Petitpas, Fabien, E-mail: fabien.petitpas@univ-amu.fr; Daniel, Eric, E-mail: eric.daniel@univ-amu.fr

2017-04-01

A new model for interface problems with capillary effects in compressible fluids is presented together with a specific numerical method to treat capillary flows and pressure waves propagation. This new multiphase model is in agreement with physical principles of conservation and respects the second law of thermodynamics. A new numerical method is also proposed where the global system of equations is split into several submodels. Each submodel is hyperbolic or weakly hyperbolic and can be solved with an adequate numerical method. This method is tested and validated thanks to comparisons with analytical solutions (Laplace law) and with experimental results onmore » droplet breakup induced by a shock wave.« less

Active ultrasonic cross-correlation flowmeters for mixed-phase pipe flows

NASA Astrophysics Data System (ADS)

Sheen, S. H.; Raptis, A. C.

Two ultrasonic flowmeters which employ the active cross-correlation technique and use a simple clamp-on transducer arrangement are discussed. The flowmeter for solid/liquid flows was tested over a wide range of coal concentration in water and oil. The measured velocity based on the peak position of the cross-correlation function is consistently higher by about 15% than the average velocity measured by flow diversion. The origin of the difference results mainly from the flow velocity profiles and the transit-time probability distribution. The flowmeter that can measure particle velocity in a solid/gas flow requires acoustic decoupling arrangement between two sensing stations. The measured velocity is mainly associated with the particles near the wall. Performance of both flowmeters is presented.

Laboratory-based observations of capillary barriers and preferential flow in layered snow

NASA Astrophysics Data System (ADS)

Avanzi, F.; Hirashima, H.; Yamaguchi, S.; Katsushima, T.; De Michele, C.

2015-12-01

Several evidences are nowadays available that show how the effects of capillary gradients and preferential flow on water transmission in snow may play a more important role than expected. To observe these processes and to contribute in their characterization, we performed observations on the development of capillary barriers and preferential flow patterns in layered snow during cold laboratory experiments. We considered three different layering (all characterized by a finer-over-coarser texture in grain size) and three different water input rates. Nine samples of layered snow were sieved in a cold laboratory, and subjected to a constant supply of dyed tracer. By means of visual inspection, horizontal sectioning and liquid water content measurements, the processes of ponding and preferential flow were characterized as a function of texture and water input rate. The dynamics of each sample were replicated using the multi-layer physically-based SNOWPACK model. Results show that capillary barriers and preferential flow are relevant processes ruling the speed of liquid water in stratified snow. Ponding is associated with peaks in LWC at the boundary between the two layers equal to ~ 33-36 vol. % when the upper layer is composed by fine snow (grain size smaller than 0.5 mm). The thickness of the ponding layer at the textural boundary is between 0 and 3 cm, depending on sample stratigraphy. Heterogeneity in water transmission increases with grain size, while we do not observe any clear dependency on water input rate. The extensive comparison between observed and simulated LWC profiles by SNOWPACK (using an approximation of Richards Equation) shows high performances by the model in estimating the LWC peak over the boundary, while water speed in snow is underestimated by the chosen water transport scheme.

Hydrodynamic sample injection into short electrophoretic capillary in systems with a flow-gating interface.

PubMed

Opekar, František; Tůma, Petr

2017-01-13

An electrophoretic apparatus with a flow-gating interface has been developed, enabling hydrodynamic sequence injection of the sample into the separation capillary from the liquid flow by underpressure generated in the outlet electrophoretic vessel. The properties of the apparatus were tested on an artificial sample of an equimolar mixture of 100μM potassium and sodium ions and arginine. The repeatability of the injection of the tested ions expressed as RSD (in%) for the peak area, peak height and migration time was in the range 0.76-2.08, 0.18-0.68 and 0.28-0.48, respectively. Under optimum conditions, the apparatus was used for sequence monitoring of the reaction between the antidiabetic drug phenyl biguanide and the glycation agent methyl glyoxal. The reaction solution was continuously sampled by a microdialysis probe from a thermostated external vessel using a syringe pump at a flow rate of 3μLmin -1 and was injected into a separation capillary at certain time intervals. The electrophoretic separation progressed in a capillary with an internal diameter of 50μm with a length of 11.5cm and was monitored using a contactless conductivity detector. Copyright © 2016 Elsevier B.V. All rights reserved.

Effect of anti-vertigo granule on the opening number and blood flow of mouse ear capillary network

NASA Astrophysics Data System (ADS)

Li, Chongxian; Liu, Xiaobin; Li, Jun; Hao, Shaojun; Wang, Xidong; Li, Wenjun; Zhang, Zhengchen

2018-04-01

To observe the effects of anti-glare particles on the open number and blood flow in the auricle of mice with microcirculation disturbance model. Sixty mice, half male and half female, were randomly divided into 6 groups. The mice were given Kangxuan granule suspension, serum brain granule suspension and normal saline of the same volume, respectively, once a day. The mice were anesthetized by intraperitoneal injection of chloral hydrate at 1 hour after the last administration. The mouse was fixed on the observation platform and the auricle was placed on the transmission stage. BZ-2000 microcirculation microscope and microcirculation analysis system were used to observe the changes of blood velocity and capillary opening volume in auricle of mice before administration. The changes of blood velocity and capillaries opening volume of mouse auricle were observed 2 min after epinephrine injection into tail vein of mice. Bear fruit: Compared with those before epinephrine, the opening number of capillary reticulum of auricle in large dose Kangxuan granule group was significantly decreased (P<0.05), and in normal saline group and middle group. In the small dose Kangxuan granule group, the opening number of capillary network of auricle decreased significantly (P<0.01). Compared with the model group, the large dose Kangxuan granule group could significantly increase the opening number of the auricle capillary network in mice (P<0.01). Yangxuannao granule group could significantly increase the opening number of auricle capillary reticulum in mice (P<0.05), compared with the model group by Ridit test. Both Kangxuan granule group and Yangxuannao granule group could significantly improve the auricle hair of mice with microcirculation disorder. The blood flow in fine blood vessels (P<0.01). Kangxuan granule has a good effect on the opening number of capillary network of auricle and blood flow in mice with microcirculation disorder.

Subtle exchange model of flow depended on the blood cell shape to enhance the micro-circulation in capillary

NASA Astrophysics Data System (ADS)

Chan, Iatneng

2012-02-01

In general the exchange of gases or other material in capillary system is conceptualized by the diffusion effect. But in this model, we investigate a micro-flow pattern by simulation and computation on a micro-exchange model in which the blood cell is a considered factor, especially on its shape. It shows that the cell benefits the circulation while it is moving in the capillary. In the study, the flow detail near the cell surface is mathematically analyzed, such that the Navier-Stokes equations are applied and the viscous factor is also briefly considered. For having a driven force to the motion of micro-circulation, a breathing mode is suggested to approximately compute on the flow rate in the blood capillary during the transfer of cell. The rate is also used to estimate the enhancement to the circulation in additional to the outcome of diffusion. Moreover in the research, the shape change of capillary wall under pressure influence is another element in the beginning calculation for the effect in the assistance to cell motion.

Direct visualization of minimal cerebral capillary flow during retrograde cerebral perfusion: an intravital fluorescence microscopy study in pigs.

PubMed

Duebener, Lennart F; Hagino, Ikuo; Schmitt, Katharina; Sakamoto, Takahiko; Stamm, Christof; Zurakowski, David; Schäfers, Hans-Joachim; Jonas, Richard A

2003-04-01

Retrograde cerebral perfusion (RCP) is used in some centers during aortic arch surgery for brain protection during hypothermic circulatory arrest. It is still unclear however whether RCP provides adequate microcirculatory blood flow at a capillary level. We used intravital microscopy to directly visualize the cerebral capillary blood flow in a piglet model of RCP. Twelve pigs (weight 9.7 +/- 0.9 kg) were divided into two groups (n = 6 each): deep hypothermic circulatory arrest (DHCA) and RCP. After the creation of a window over the parietal cerebral cortex, pigs underwent 10 minutes of normothermic bypass and 40 minutes of cooling to 15 degrees C on cardiopulmonary bypass ([CPB] pH-stat, hemocrit 30%, pump flow 100 mL x kg(-1) x min(-1)). This was followed by 45 minutes of DHCA and rewarming on CPB to 37 degrees C. In the RCP group the brain was retrogradely perfused (pump flow 30 mL x kg(-1) x min(-1)) during DHCA through the superior vena cava after inferior vena cava occlusion. Plasma was labeled with fluorescein-isothiocyanate-dextran for assessing microvascular diameter and functional capillary density (FCD), defined as total length of erythrocyte-perfused capillaries per observation area. Cerebral tissue oxygenation was determined by nicotinamide adenine dinucleotide hydrogen (NADH) autofluorescence, which increases during tissue ischemia. During normothermic and hypothermic antegrade cerebral perfusion the FCD did not significantly change from base line (97% +/- 14% and 96% +/- 12%, respectively). During retrograde cerebral perfusion the FCD decreased highly significantly to 2% +/- 2% of base line values (p < 0.001). Thus there was no evidence of significant capillary blood flow during retrograde cerebral perfusion. The microvascular diameter of cerebral arterioles that were slowly perfused significantly decreased to 27% +/- 6% of base line levels during RCP. NADH fluorescence progressively and significantly increased during RCP, indicating poorer tissue

A continuous-flow capillary mixing method to monitor reactions on the microsecond time scale.

PubMed Central

Shastry, M C; Luck, S D; Roder, H

1998-01-01

A continuous-flow capillary mixing apparatus, based on the original design of Regenfuss et al. (Regenfuss, P., R. M. Clegg, M. J. Fulwyler, F. J. Barrantes, and T. M. Jovin. 1985. Rev. Sci. Instrum. 56:283-290), has been developed with significant advances in mixer design, detection method and data analysis. To overcome the problems associated with the free-flowing jet used for observation in the original design (instability, optical artifacts due to scattering, poor definition of the geometry), the solution emerging from the capillary is injected directly into a flow-cell joined to the tip of the outer capillary via a ground-glass joint. The reaction kinetics are followed by measuring fluorescence versus distance downstream from the mixer, using an Hg(Xe) arc lamp for excitation and a digital camera with a UV-sensitized CCD detector for detection. Test reactions involving fluorescent dyes indicate that mixing is completed within 15 micros of its initiation and that the dead time of the measurement is 45 +/- 5 micros, which represents a >30-fold improvement in time resolution over conventional stopped-flow instruments. The high sensitivity and linearity of the CCD camera have been instrumental in obtaining artifact-free kinetic data over the time window from approximately 45 micros to a few milliseconds with signal-to-noise levels comparable to those of conventional methods. The scope of the method is discussed and illustrated with an example of a protein folding reaction. PMID:9591695

Regional myocardial flow and capillary permeability-surface area products are nearly proportional.

PubMed

Caldwell, J H; Martin, G V; Raymond, G M; Bassingthwaighte, J B

1994-08-01

Analyses of data on the transcapillary exchange and cellular uptake in the normal heart have generally been based on the assumption that local membrane conductances and volumes of distribution are everywhere the same. The question is whether such an assumption is justified in view of the marked (sixfold) heterogeneity of local blood flows per gram tissue. The method was to estimate both flow and capillary membrane permeability-surface area products (PS) locally in the heart. For each of five dogs running on a sloped treadmill, the deposition of tracer microspheres and of [131I]iodophenylpentadecanoic acid (IPPA), after left atrial injection, was determined in 256 pieces of left ventricular myocardium by killing the animals at approximately 100 s after radiotracer injection. A hydraulic occluder stopped the flow to a portion of the myocardium supplied by the left circumflex coronary artery 30 s before tracer injection. Regional flows ranged from 0.1 to 7.0 ml.g-1.min-1. IPPA extractions ranged from 20 to 49%. Using the known flows, we assumed the applicability of an axially distributed blood-tissue exchange model to estimate the PS for the capillary (PSc) and the parenchymal cell. It was impossible to explain the data if the PSc values for membrane transport were uniform throughout the organ. Rather, the only reasonable descriptors of the data required that local PSc values increase with local flow, almost in proportion. Current methods of analysis using data based on deposition methods need to be revised to take into account the near proportionality of PS to flow for at least some substrates.

Flow-through micro-capillary refractive index sensor based on T/R spectral shift monitoring.

PubMed

Rigamonti, Giulia; Guardamagna, Marco; Bello, Valentina; Marconi, Stefania; Auricchio, Ferdinando; Merlo, Sabina

2017-10-01

We present a flow-through refractive index sensor for measuring the concentration of glucose solutions based on the application of rectangular glass micro-capillaries, with channel depth of 50 µm and 30 µm. A custom designed and 3D printed polymeric shell protects the tiny capillaries, ensuring an easier handling and interconnection with the macro-fluidic path. By illuminating the capillary with broadband radiation centered at λ~1.55 µm, both the transmitted (T) and reflected (R) optical spectrum from the capillary are detected with an optical spectrum analyzer, exploiting an all-fiber setup. Monitoring the spectral shift of the ratio T/R in response to increasing concentration of glucose solutions in water we have obtained sensitivities up to 530.9 nm/RIU and limit of detection in the range of 10 -5 -10 -4 RIU. Experimental results are in agreement with the theoretically predicted principle of operation. After the demonstration of amplitude detection at a single wavelength, we finally discuss the impact of the capillary parameters on the sensitivity.

Multiple capillary biochemical analyzer

DOEpatents

Dovichi, N.J.; Zhang, J.Z.

1995-08-08

A multiple capillary analyzer allows detection of light from multiple capillaries with a reduced number of interfaces through which light must pass in detecting light emitted from a sample being analyzed, using a modified sheath flow cuvette. A linear or rectangular array of capillaries is introduced into a rectangular flow chamber. Sheath fluid draws individual sample streams through the cuvette. The capillaries are closely and evenly spaced and held by a transparent retainer in a fixed position in relation to an optical detection system. Collimated sample excitation radiation is applied simultaneously across the ends of the capillaries in the retainer. Light emitted from the excited sample is detected by the optical detection system. The retainer is provided by a transparent chamber having inward slanting end walls. The capillaries are wedged into the chamber. One sideways dimension of the chamber is equal to the diameter of the capillaries and one end to end dimension varies from, at the top of the chamber, slightly greater than the sum of the diameters of the capillaries to, at the bottom of the chamber, slightly smaller than the sum of the diameters of the capillaries. The optical system utilizes optic fibers to deliver light to individual photodetectors, one for each capillary tube. A filter or wavelength division demultiplexer may be used for isolating fluorescence at particular bands. 21 figs.

Multiple capillary biochemical analyzer

DOEpatents

Dovichi, Norman J.; Zhang, Jian Z.

1995-01-01

A multiple capillary analyzer allows detection of light from multiple capillaries with a reduced number of interfaces through which light must pass in detecting light emitted from a sample being analyzed, using a modified sheath flow cuvette. A linear or rectangular array of capillaries is introduced into a rectangular flow chamber. Sheath fluid draws individual sample streams through the cuvette. The capillaries are closely and evenly spaced and held by a transparent retainer in a fixed position in relation to an optical detection system. Collimated sample excitation radiation is applied simultaneously across the ends of the capillaries in the retainer. Light emitted from the excited sample is detected by the optical detection system. The retainer is provided by a transparent chamber having inward slanting end walls. The capillaries are wedged into the chamber. One sideways dimension of the chamber is equal to the diameter of the capillaries and one end to end dimension varies from, at the top of the chamber, slightly greater than the sum of the diameters of the capillaries to, at the bottom of the chamber, slightly smaller than the sum of the diameters of the capillaries. The optical system utilizes optic fibres to deliver light to individual photodetectors, one for each capillary tube. A filter or wavelength division demultiplexer may be used for isolating fluorescence at particular bands.

Application of the ultrasonic technique and high-speed filming for the study of the structure of air-water bubbly flows

DOE Office of Scientific and Technical Information (OSTI.GOV)

Carvalho, R.D.M.; Venturini, O.J.; Tanahashi, E.I.

2009-10-15

Multiphase flows are very common in industry, oftentimes involving very harsh environments and fluids. Accordingly, there is a need to determine the dispersed phase holdup using noninvasive fast responding techniques; besides, knowledge of the flow structure is essential for the assessment of the transport processes involved. The ultrasonic technique fulfills these requirements and could have the capability to provide the information required. In this paper, the potential of the ultrasonic technique for application to two-phase flows was investigated by checking acoustic attenuation data against experimental data on the void fraction and flow topology of vertical, upward, air-water bubbly flows inmore » the zero to 15% void fraction range. The ultrasonic apparatus consisted of one emitter/receiver transducer and three other receivers at different positions along the pipe circumference; simultaneous high-speed motion pictures of the flow patterns were made at 250 and 1000 fps. The attenuation data for all sensors exhibited a systematic interrelated behavior with void fraction, thereby testifying to the capability of the ultrasonic technique to measure the dispersed phase holdup. From the motion pictures, basic gas phase structures and different flows patterns were identified that corroborated several features of the acoustic attenuation data. Finally, the acoustic wave transit time was also investigated as a function of void fraction. (author)« less

An accessible micro-capillary electrophoresis device using surface-tension-driven flow

PubMed Central

Mohanty, Swomitra K.; Warrick, Jay; Gorski, Jack; Beebe, David J.

2010-01-01

We present a rapidly fabricated micro-capillary electrophoresis chip that utilizes surface-tension-driven flow for sample injection and extraction of DNA. Surface-tension-driven flow (i.e. passive pumping) injects a fixed volume of sample that can be predicted mathematically. Passive pumping eliminates the need for tubing, valves, syringe pumps, and other equipment typically needed for interfacing with microelectrophoresis chips. This method requires a standard micropipette to load samples before separation, and remove the resulting bands after analysis. The device was made using liquid phase photopolymerization to rapidly fabricate the chip without the need of special equipment typically associated with the construction of microelectrophoresis chips (e.g. cleanroom). Batch fabrication time for the device presented here was 1.5 h including channel coating time to suppress electroosmotic flow. Devices were constructed out of poly-isobornyl acrylate and glass. A standard microscope with a UV source was used for sample detection. Separations were demonstrated using Promega BenchTop 100 bp ladder in hydroxyl ethyl cellulose (HEC) and oligonucleotides of 91 and 118 bp were used to characterize sample injection and extraction of DNA bands. The end result was an inexpensive micro-capillary electrophoresis device that uses tools (e.g. micropipette, electrophoretic power supplies, and microscopes) already present in most labs for sample manipulation and detection, making it more accessible for potential end users. PMID:19425002

Capillary trapping of particles in thin-film flows

NASA Astrophysics Data System (ADS)

Dressaire, Emilie; Gomez, Michael; Colnet, Benedicte; Sauret, Alban

2017-11-01

When a thin layer of suspension flows over a substrate, some particles remain trapped on the solid surface. When the thickness of the liquid layer is comparable to the particle size, the particles deform the liquid interface, which leads to local interactions. These effects modify the transport of particles and the dynamics of the liquid films. Here, we characterize how capillary interactions affect the transport and deposition of non-Brownian particles moving in thin liquid films and the resulting loss of transported material. We focus on gravitational drainage flows, in which the film thickness becomes comparable to the particle size. Depending on the concentration of particles, we find that the drainage dynamics exhibits behavior that cannot be captured with a continuum model, due to the deposition of particles on the substrate. ANR-16-CE30-0009 & CNRS-PICS-07242 & ACS-PRF 55845-ND9.

Evaluation of laser Doppler flowmetry for measurement of capillary blood flow in the stomach wall of dogs during gastric dilatation-volvulus.

PubMed

Monnet, Eric; Pelsue, Davyd; MacPhail, Catriona

2006-02-01

To validate laser doppler flowmetry (LDF) for measurement of blood flow in the stomach wall of dogs with gastric dilatation-volvulus (GDV). Six purpose-bred dogs and 24 dogs with naturally occurring GDV. Experimental and clinical. Capillary blood flow in the body of the stomach and pyloric antrum was measured with LDF (tissue perfusion unit (TPU) before and after induction of portal hypertension (PH) and after PH plus gastric ischemia (GI; PH + GI) and compared with flow measured by colored microsphere technique. Capillary flow was measured by LDF in the stomach wall of dogs with GDV. PH and PH+GI induced a significant reduction in blood flow in the body of the stomach (P = .019). A significant positive correlation was present between percent changes in capillary blood flow measured by LDF and colored microspheres after induction of PH + GI in the body of the stomach (r = 0.94, P = .014) and in the pyloric antrum (r = 0.95, P = .049). Capillary blood flow measured in the body of the stomach of 6 dogs that required partial gastrectomy (5.00+/-3.30 TPU) was significantly lower than in dogs that did not (28.00+/-14.40 TPU, P = .013). LDF can detect variations in blood flow in the stomach wall of dogs. LDF may have application for evaluation of stomach wall viability during surgery in dogs with GDV.

Non-Invasive Characterization Of A Flowing Multi-Phase Fluid Using Ultrasonic Interferometry

DOEpatents

Sinha, Dipen N.

2005-11-01

An apparatus for noninvasively monitoring the flow and/or the composition of a flowing liquid using ultrasound is described. The position of the resonance peaks for a fluid excited by a swept-frequency ultrasonic signal have been found to change frequency both in response to a change in composition and in response to a change in the flow velocity thereof. Additionally, the distance between successive resonance peaks does not change as a function of flow, but rather in response to a change in composition. Thus, a measurement of both parameters (resonance position and resonance spacing), once calibrated, permits the simultaneous determination of flow rate and composition using the apparatus and method of the present invention.

Experimental study of streaming flows associated with ultrasonic levitators

NASA Astrophysics Data System (ADS)

Trinh, E. H.; Robey, J. L.

1994-11-01

Steady-state acoustic streaming flow patterns have been observed during the operation of a variety of resonant single-axis ultrasonic levitators in a gaseous environment and in the 20-37 kHz frequency range. Light sheet illumination and scattering from smoke particles have revealed primary streaming flows which display different characteristics at low and high sound pressure levels. Secondary macroscopic streaming cells around levitated samples are superimposed on the primary streaming flow pattern generated by the standing wave. These recorded flows are quite reproducible, and are qualitatively the same for a variety of levitator physical geometries. An onset of flow instability can also be recorded in nonisothermal systems, such as levitated spot-heated samples when the resonance conditions are not exactly satisfied. A preliminary qualitative interpretation of these experimental results is presented in terms of the superposition of three discrete sets of circulation cells operating on different spatial scales. These relevant length scales are the acoustic wavelength, the levitated sample size, and finally the acoustic boundary layer thickness. This approach fails, however, to explain the streaming flow-field morphology around liquid drops levitated on Earth. Observation of the interaction between the flows cells and the levitated samples also suggests the existence of a steady-state torque induced by the streaming flows.

Fourier Collocation Approach With Mesh Refinement Method for Simulating Transit-Time Ultrasonic Flowmeters Under Multiphase Flow Conditions.

PubMed

Simurda, Matej; Duggen, Lars; Basse, Nils T; Lassen, Benny

2018-02-01

A numerical model for transit-time ultrasonic flowmeters operating under multiphase flow conditions previously presented by us is extended by mesh refinement and grid point redistribution. The method solves modified first-order stress-velocity equations of elastodynamics with additional terms to account for the effect of the background flow. Spatial derivatives are calculated by a Fourier collocation scheme allowing the use of the fast Fourier transform, while the time integration is realized by the explicit third-order Runge-Kutta finite-difference scheme. The method is compared against analytical solutions and experimental measurements to verify the benefit of using mapped grids. Additionally, a study of clamp-on and in-line ultrasonic flowmeters operating under multiphase flow conditions is carried out.

Capillary trapping in thin-film flows of particles

NASA Astrophysics Data System (ADS)

Sauret, Alban; Gomez, Michael; Dressaire, Emilie

Flows of suspensions have been modeled on a continuum level by using constitutive relations to capture how the viscosity varies with the particle concentration. However, in thin liquid films, where the thickness of the liquid layer is comparable to the particle size, the particles deform the liquid interface, which leads to local interactions. These effects modify the transport of particles and could result in the contamination of the surface and the loss of transported material. Here, we characterize how capillary interactions affect the transport and deposition of non-Brownian particles moving in thin liquid films. We focus on gravitational drainage flows, in which the film thickness becomes comparable to the particle size. Depending on the concentration of particles, we find that the dynamics of the drainage exhibits behavior that cannot be captured with a Newtonian model, due to the deposition of particles on the substrate. ANR-16-CE30-0009 and CNRS-PICS-07242.

Prototyping of Poly(dimethylsiloxane) Interfaces for Flow Gating, Reagent Mixing, and Tubing Connection in Capillary Electrophoresis

PubMed Central

Zhang, Qiyang; Gong, Maojun

2014-01-01

Integrated microfluidic systems coupled with electrophoretic separations have broad application in biological and chemical analysis. Interfaces for the connection of various functional parts play a major role in the performance of a system. Here we developed a rapid prototyping method to fabricate monolithic poly(dimethylsiloxane) (PDMS) Interfaces for flow-gated injection, online reagent mixing, and tube-to-tube connection in an integrated capillary electrophoresis (CE) system. The basic idea was based on the properties of PDMS: elasticity, transparency, and suitability for prototyping. The molds for these interfaces were prepared by using commercially available stainless steel wires and nylon lines or silica capillaries. A steel wire was inserted through the diameter of a nylon line and a cross format was obtained as the mold for PDMS casting of flow gates and 4-way mixers. These interfaces accommodated tubing connection through PDMS elasticity and provided easy visual trouble shooting. The flow gate used smaller channel diameters thus reducing flow rate by 25 fold for effective gating compared with mechanically machined counterparts. Both PDMS mixers and the tube-to-tube connectors could minimize the sample dead volume by using an appropriate capillary configuration. As a whole, the prototyped PDMS interfaces are reusable, inexpensive, convenient for connection, and robust when integrated with the CE detection system. Therefore, these interfaces could see potential applications in CE and CE-coupled systems. PMID:24331370

Flow rate impacts on capillary pressure and interface curvature of connected and disconnected fluid phases during multiphase flow in sandstone

NASA Astrophysics Data System (ADS)

Herring, Anna L.; Middleton, Jill; Walsh, Rick; Kingston, Andrew; Sheppard, Adrian

2017-09-01

We investigate capillary pressure-saturation (PC-S) relationships for drainage-imbibition experiments conducted with air (nonwetting phase) and brine (wetting phase) in Bentheimer sandstone cores. Three different flow rate conditions, ranging over three orders of magnitude, are investigated. X-ray micro-computed tomographic imaging is used to characterize the distribution and amount of fluids and their interfacial characteristics. Capillary pressure is measured via (1) bulk-phase pressure transducer measurements, and (2) image-based curvature measurements, calculated using a novel 3D curvature algorithm. We distinguish between connected (percolating) and disconnected air clusters: curvatures measured on the connected phase interfaces are used to validate the curvature algorithm and provide an indication of the equilibrium condition of the data; curvature and volume distributions of disconnected clusters provide insight to the snap-off processes occurring during drainage and imbibition under different flow rate conditions.

Turbulent slurry flow measurement using ultrasonic Doppler method in rectangular pipe

NASA Astrophysics Data System (ADS)

Bareš, V.; Krupička, J.; Picek, T.; Brabec, J.; Matoušek, V.

2014-03-01

Distribution of velocity and Reynolds stress was measured using ultrasonic velocimetry in flows of water and Newtonian water-ballotini slurries in a pressurized Plexiglas pipe. Profiles of the measured parameters were sensed in the vertical plane at the centreline of a rectangular cross section of the pipe. Reference measurements in clear water produced expected symmetrical velocity profiles the shape of which was affected by secondary currents developed in the rectangular pipe. Slurry-flow experiments provided information on an effect of the concentration of solid grains on the internal structure of the flow. Strong attenuation of velocity fluctuations caused by a presence of grains was identified. The attenuation increased with the increasing local concentration of the grains.

Hydrodynamic flow in capillary-channel fiber columns for liquid chromatography.

PubMed

Stanelle, Rayman D; Sander, Lane C; Marcus, R Kenneth

2005-12-23

The flow characteristics of capillary-channel polymer (C-CP) fiber liquid chromatographic (LC) columns have been investigated. The C-CP fibers are manufactured with eight longitudinal grooves (capillary channels) extending the length of the fibers. Three C-CP fiber examples were studied, with fiber dimensions ranging from approximately 35 microm to 65 microm, and capillary-channel dimensions ranging from approximately 6 microm to 35 microm. The influence of fiber packing density and column inner diameter on peak asymmetry, peak width, and run-to-run reproducibility have been studied for stainless steel LC columns packed with polyester (PET) and polypropylene (PP) C-CP fibers. The van Deemter A-term was evaluated as a function of fiber packing density (approximately 0.3 g/cm(3)-0.75 g/cm(3)) for columns of 4.6 mm inner diameter (i.d.) and at constant packing densities for 1.5 mm, 3.2 mm, 4.6 mm, and 7.7 mm i.d. columns. Although column diameter had little influence on the eluting peak widths, peak asymmetry increased with increasing column diameter. The A-terms for the C-CP fiber packed columns are somewhat larger than current commercial, microparticulate-packed columns, and means for improvement are discussed. Applications in the area of protein (macromolecule) separations appear the most promising at this stage of the system development.

The inclusion of capillary distribution in the adiabatic tissue homogeneity model of blood flow

NASA Astrophysics Data System (ADS)

Koh, T. S.; Zeman, V.; Darko, J.; Lee, T.-Y.; Milosevic, M. F.; Haider, M.; Warde, P.; Yeung, I. W. T.

2001-05-01

We have developed a non-invasive imaging tracer kinetic model for blood flow which takes into account the distribution of capillaries in tissue. Each individual capillary is assumed to follow the adiabatic tissue homogeneity model. The main strength of our new model is in its ability to quantify the functional distribution of capillaries by the standard deviation in the time taken by blood to pass through the tissue. We have applied our model to the human prostate and have tested two different types of distribution functions. Both distribution functions yielded very similar predictions for the various model parameters, and in particular for the standard deviation in transit time. Our motivation for developing this model is the fact that the capillary distribution in cancerous tissue is drastically different from in normal tissue. We believe that there is great potential for our model to be used as a prognostic tool in cancer treatment. For example, an accurate knowledge of the distribution in transit times might result in an accurate estimate of the degree of tumour hypoxia, which is crucial to the success of radiation therapy.

Progression of Diabetic Capillary Occlusion: A Model

PubMed Central

Gens, John Scott; Glazier, James A.; Burns, Stephen A.; Gast, Thomas J.

2016-01-01

An explanatory computational model is developed of the contiguous areas of retinal capillary loss which play a large role in diabetic maculapathy and diabetic retinal neovascularization. Strictly random leukocyte mediated capillary occlusion cannot explain the occurrence of large contiguous areas of retinal ischemia. Therefore occlusion of an individual capillary must increase the probability of occlusion of surrounding capillaries. A retinal perifoveal vascular sector as well as a peripheral retinal capillary network and a deleted hexagonal capillary network are modelled using Compucell3D. The perifoveal modelling produces a pattern of spreading capillary loss with associated macular edema. In the peripheral network, spreading ischemia results from the progressive loss of the ladder capillaries which connect peripheral arterioles and venules. System blood flow was elevated in the macular model before a later reduction in flow in cases with progression of capillary occlusions. Simulations differing only in initial vascular network structures but with identical dynamics for oxygen, growth factors and vascular occlusions, replicate key clinical observations of ischemia and macular edema in the posterior pole and ischemia in the retinal periphery. The simulation results also seem consistent with quantitative data on macular blood flow and qualitative data on venous oxygenation. One computational model applied to distinct capillary networks in different retinal regions yielded results comparable to clinical observations in those regions. PMID:27300722

A novel sidestream ultrasonic flow sensor for multiple breath washout in children.

PubMed

Fuchs, Susanne I; Sturz, J; Junge, S; Ballmann, M; Gappa, M

2008-08-01

Inert gas multiple breath washout (MBW) for measuring Lung Clearance Index using mass spectrometry and 4% sulfur hexafluoride (SF(6)) as the tracer gas has been shown to be sensitive for detecting early Cystic Fibrosis (CF) lung disease. However, mass spectrometry requires bulky equipment and is expensive to buy and maintain. A novel sidestream ultrasonic device may overcome this problem. The aims of this study were to assess the feasibility and clinical validity of measuring lung volume (functional residual capacity, FRC) and the LCI using the sidestream ultrasonic flow sensor in children and adolescents with CF in relation to spirometry and plain chest radiographs. MBW using the sidestream ultrasonic device and conventional spirometry were performed in 26 patients with CF and 22 healthy controls. In the controls (4.7-17.7 years) LCI was similar to that reported using mass spectrometry (mean (SD) 6.7 (0.5)). LCI was elevated in 77% of the CF children (6.8-18.9 years), whereas spirometry was abnormal in only 38.5%, 61.5%, and 26.9% for FEV(1), MEF(25), and FEV(1)/FVC, respectively. This was more marked in children <10 years. LCI correlated with the Crispin-Norman score, whereas FEV(1) did not. Sidestream ultrasonic MBW is a valid and simple alternative to mass spectrometry for assessing ventilation homogeneity in children. (c) 2008 Wiley-Liss, Inc.

Instability of the capillary bridge

NASA Astrophysics Data System (ADS)

Pare, Gounseti; Hoepffner, Jerome

2014-11-01

Capillary adhesion is a physical mechanism that maintains two bodies in contact by capillarity through a liquid ligament. The capillary bridge is an idealization of this capillary adhesion. In this study we first focus on the classical case of the stability of the capillary bridge. Secondly we study a slightly more complex configuration, imagining a flow in the capillary bridge as in the case of the dynamics of the neck of a liquid ligament, in its withdrawal under the effect of capillarity. Inspired by the experiments on soap films of Plateau, the configuration analyzed consists of an initially axisymmetric, mass of fluid held by surface tension forces between two parallel, coaxial, solid pipes of the same diameter. The results presented are obtained by numerical simulations using the free software, Gerris Flow Solver. We first focus on the capillary Venturi. In the static configuration the stability diagram of the capillary bridge obtained is in perfect agreement with the results of Lev A. Slobozhanin. In the dynamic case we develop a matlab code based on the one dimensional equations of Eggers and Dupont. The comparison of the bifurcation diagram obtained and the numerical simulations shows a good agreement.

Capillarics: pre-programmed, self-powered microfluidic circuits built from capillary elements.

PubMed

Safavieh, Roozbeh; Juncker, David

2013-11-07

Microfluidic capillary systems employ surface tension effects to manipulate liquids, and are thus self-powered and self-regulated as liquid handling is structurally and chemically encoded in microscale conduits. However, capillary systems have been limited to perform simple fluidic operations. Here, we introduce complex capillary flow circuits that encode sequential flow of multiple liquids with distinct flow rates and flow reversal. We first introduce two novel microfluidic capillary elements including (i) retention burst valves and (ii) robust low aspect ratio trigger valves. These elements are combined with flow resistors, capillary retention valves, capillary pumps, and open and closed reservoirs to build a capillary circuit that, following sample addition, autonomously delivers a defined sequence of multiple chemicals according to a preprogrammed and predetermined flow rate and time. Such a circuit was used to measure the concentration of C-reactive protein. This work illustrates that as in electronics, complex capillary circuits may be built by combining simple capillary elements. We define such circuits as "capillarics", and introduce symbolic representations. We believe that more complex circuits will become possible by expanding the library of building elements and formulating abstract design rules.

Kinetics of gravity-driven slug flow in partially wettable capillaries of varying cross section

NASA Astrophysics Data System (ADS)

Nissan, Alon; Wang, Qiuling; Wallach, Rony

2016-11-01

A mathematical model for slug (finite liquid volume) motion in not-fully-wettable capillary tubes with sinusoidally varying cross-sectional areas was developed. The model, based on the Navier-Stokes equation, accounts for the full viscous terms due to nonuniform geometry, the inertial term, the slug's front and rear meniscus hysteresis effect, and dependence of contact angle on flow velocity (dynamic contact angle). The model includes a velocity-dependent film that is left behind the advancing slug, reducing its mass. The model was successfully verified experimentally by recording slug movement in uniform and sinusoidal capillary tubes with a gray-scale high-speed camera. Simulation showed that tube nonuniformity has a substantial effect on slug flow pattern: in a uniform tube it is monotonic and depends mainly on the slug's momentary mass/length; an undulating tube radius results in nonmonotonic flow characteristics. The static nonzero contact angle varies locally in nonuniform tubes owing to the additional effect of wall slope. Moreover, the nonuniform cross-sectional area induces slug acceleration, deceleration, blockage, and metastable-equilibrium locations. Increasing contact angle further amplifies the geometry effect on slug propagation. The developed model provides a modified means of emulating slug flow in differently wettable porous media for intermittent inlet water supply (e.g., raindrops on the soil surface).

Decreased retinal capillary flow is not a mediator of the protective myopia-diabetic retinopathy relationship.

PubMed

Man, Ryan Eyn Kidd; Sasongko, Muhammad Bayu; Xie, Jing; Best, William J; Noonan, Jonathan E; Lo, Tiffany Ching Shen; Wang, Jie Jin; Luu, Chi D; Lamoureux, Ecosse L

2014-09-30

The mechanisms supporting the protective relationship between a longer axial length (AL) and a decreased risk of diabetic retinopathy (DR) remain unclear. Previous studies have demonstrated reduced retinal blood flow in axial myopia, and it has been suggested that the compromised retinal capillaries in diabetes are less likely to leak and rupture as a result of this decreased flow. In this study, we therefore investigated if reduced retinal capillary flow (RCF) is a potential mechanism underpinning this protective relationship. Retinal capillary flow was assessed using the Heidelberg Retinal Flowmeter in 150 eyes of 85 patients with diabetes aged 18+ years from the Royal Victorian Eye and Ear Hospital and St. Vincent's Hospital (Melbourne), Australia. Axial length was measured using the Intraocular Lens Master. Diabetic retinopathy was graded from two-field retinal photographs into none, mild, moderate, and severe DR using the modified Airlie House classification system. A total of 74 out of 150 eyes (49.3%) had DR. A longer AL was associated with decreased odds of DR presence (per mm increase in AL, odds ratio [OR] 0.61, 95% confidence interval [CI] 0.41-0.91) and DR severity (OR: 0.65; 95% CI: 0.44-0.95). However, no association was found between AL and RCF (per mm increase in AL, regression coefficient [β] -1.80, 95% CI -13.50 to 9.50) or between RCF and DR (per unit increase in RCF, OR 1.00; 95% CI 0.99-1.00). Our finding suggests that diminished RCF may not be a major factor underlying the protective association between axial elongation and DR. Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.

Effect of hindpaw electrical stimulation on capillary flow heterogeneity and oxygen delivery (Conference Presentation)

NASA Astrophysics Data System (ADS)

Li, Yuandong; Wei, Wei; Li, Chenxi; Wang, Ruikang K.

2017-02-01

We report a novel use of optical coherence tomography (OCT) based angiography to visualize and quantify dynamic response of cerebral capillary flow pattern in mice upon hindpaw electrical stimulation through the measurement of the capillary transit-time heterogeneity (CTH) and capillary mean transit time (MTT) in a wide dynamic range of a great number of vessels in vivo. The OCT system was developed to have a central wavelength of 1310 nm, a spatial resolution of 8 µm and a system dynamic range of 105 dB at an imaging rate of 92 kHz. The mapping of dynamic cerebral microcirculations was enabled by optical microangiography protocol. From the imaging results, the spatial homogenization of capillary velocity (decreased CTH) was observed in the region of interest (ROI) corresponding to the stimulation, along with an increase in the MTT in the ROI to maintain sufficient oxygen exchange within the brain tissue during functional activation. We validated the oxygen consumption due to an increase of the MTT through demonstrating an increase in the deoxygenated hemoglobin (HbR) during the stimulation by the use of laser speckle contrast imaging.

Determination of parotid sulfate secretion in sheep by means of ultrasonic flow probes.

PubMed

Méot, F; Bonnet, J-M; Boivin, R; Cirio, A

2006-05-01

The bilateral output of sulfate in parotid saliva, the relationship with its plasma level and with parotid flow, and its variation according to feeding behavior were determined in ad libitum, normal-sulfate (0.28% DM)-fed sheep (n = 6) using a transit time ultrasonic flow meter system to measure salivary flow. Ultrasonic flow meter probes were bilaterally implanted, under general anesthesia, around parotid ducts previously fitted through their oral ends with nonobstructive sampling catheters. Salivary flows were continuously recorded during 24 h, and saliva and blood samples for sulfate determinations were obtained hourly. Jaw movements were monitored with the submandibular balloon technique. The sulfate concentration in parotid saliva (mean of the group = 4.9 +/- 3.7 microg/mL) showed high variability between sheep (individual means from 0.4 +/- 0.3 to 9.3 +/- 5.9 microg/mL) and averaged 12.3% of the more stable plasma level (41.2 +/- 8.1 microg/mL). Pronounced intraindividual variations were also evident (0.1 to 26.3 microg of sulphate/mL of parotid saliva), in strong association with the fluctuations of salivary output. In 4 sheep, a decreasing exponential relationship was observed between parotid sulfate concentration and salivary secretion rate (r2 = 0.36, P < 0.01). This fact and the absence of a relationship between sulfate levels in plasma and in saliva suggest a sulfate secretory process during the passage of primary saliva through the ductal tree of the gland. The greatest rates of bilateral salivary sulfate output were observed during feeding (14.1 +/- 14.0 microg/min) and rumination (12.7 +/- 11.0 microg/min). Nevertheless, 49% of the sulfate output in parotid saliva was present during rest, as a result of the length of the resting times. The contribution of parotid sulfate to the ruminal S pool was highly variable and averaged 13.2 mg/d, representing less than 1% of the S intake. In conclusion, the accurate, reliable, nonobstructive, and bilateral

Fluid mechanics of electroosmotic flow and its effect on band broadening in capillary electrophoresis.

PubMed

Ghosal, Sandip

2004-01-01

Electroosmotic flow (EOF) usually accompanies electrophoretic migration of charged species in capillary electrophoresis unless special precautions are taken to suppress it. The presence of the EOF provides certain advantages in separations. It is an alternative to mechanical pumps, which are inefficient and difficult to build at small scales, for transporting reagents and analytes on microfluidic chips. The downside is that any imperfection that distorts the EOF profile reduces the separation efficiency. In this paper, the basic facts about EOF are reviewed from the perspective of fluid mechanics and its effect on separations in free solution capillary zone electrophoresis is discussed in the light of recent advances.

The creation of hypersonic flows by a powerful impulse capillary discharge

NASA Astrophysics Data System (ADS)

Pashchina, A. S.; Karmatsky, R. E.; Klimov, A. I.

2017-11-01

The possibility of using a powerful pulsed capillary discharge to produce quasi-stationary highspeed plasma flows with characteristic Mach numbers M = 3-10 and temperatures T = 3000-6000 K has been experimentally substantiated. In a rarefied gas atmosphere ( p ∞ < 10 Torr), the transverse size of flow exceeds d < 3 cm and the duration of the working cycle can be brought to hundreds of milliseconds, which is of interest in problems of laboratory modeling of physical-chemical and gas-dynamic effects of interaction of bodies with hypersonic flows. Strong temperature nonequilibrium has been found (with the ratio between the vibrational and rotational temperatures reaching T v/ T r = 3 and more) and anomalously low values of the effective adiabatic index, which indicates an intensive formation of polyatomic molecules and condensed particles in a carbon-containing plasma.

Biomimetic Unidirectional Capillary Action

NASA Astrophysics Data System (ADS)

Rupert, Eric; Moran, Patrick; Dahl, Jason

2017-11-01

In arid environments animals require specialized adaptations to collect adequate water. The Texas horned lizard (P. cornutum) has superhydrophylic skin which draws water out of moist soil or directly from water sources. The water then makes its way into the lizard's unidirectional capillary system, made of overlapping scales, which serves to channel water to its mouth. Testing different channel geometries, repeated ``D'' shaped chambers as in Commans et al. (2015) and truncated isosceles triangle chambers, as found in P. cornutum, we show the ability to have passive, unidirectional, fluid transport. Tests were carried out with the capillaries in a horizontal configuration. While both capillary geometries produced the desired traits, the triangular chambers showed superior unidirectionality, with no observed back flow, while ``D'' chambers showed back flow under testing conditions. The chambers provided similar flow rates. These types of channel systems will find use in microfluidics, notably in medical, printing, and lab-on-chip applications.

Using passive capillary lysimeter water flux measurements to improve flow predictions in variably saturated soils.

USDA-ARS?s Scientific Manuscript database

Passive capillary lysimeters (PCLs) are uniquely suited for measuring water fluxes in variably-saturated soils. The objective of this work was to compare PCL flux measurements with simulated fluxes obtained with a calibrated unsaturated flow model. The Richards equation-based model was calibrated us...

Enhancement of ultrasonic disintegration of sewage sludge by aeration.

PubMed

Zhao, He; Zhang, Panyue; Zhang, Guangming; Cheng, Rong

2016-04-01

Sonication is an effective way for sludge disintegration, which can significantly improve the efficiency of anaerobic digestion to reduce and recycle use of sludge. But high energy consumption limits the wide application of sonication. In order to improve ultrasonic sludge disintegration efficiency and reduce energy consumption, aeration was introduced. Results showed that sludge disintegration efficiency was improved significantly by combining aeration with ultrasound. The aeration flow rate, gas bubble size, ultrasonic density and aeration timing had impacts on sludge disintegration efficiency. Aeration that used in later stage of ultrasonic irradiation with low aeration flow rate, small gas bubbles significantly improved ultrasonic disintegration sludge efficiency. At the optimal conditions of 0.4 W/mL ultrasonic irradiation density, 30 mL/min of aeration flow rate, 5 min of aeration in later stage and small gas bubbles, ultrasonic sludge disintegration efficiency was increased by 45% and one third of ultrasonic energy was saved. This approach will greatly benefit the application of ultrasonic sludge disintegration and strongly promote the treatment and recycle of wastewater sludge. Copyright © 2015. Published by Elsevier B.V.

Chitosan as cationic polyelectrolyte for the modification of electroosmotic flow and its utilization for the separation of inorganic anions by capillary zone electrophoresis.

PubMed

Takayanagi, Toshio; Motomizu, Shoji

2006-09-01

Cationic polyelectrolyte of chitosan was used for the reversal of electroosmotic flow in capillary zone electrophoresis. The chitosan was dissolved in acetic acid solution, and stable electroosmotic flow was obtained at the chitosan concentrations between 50 and 300 microg/mL. Separation of inorganic anions was carried out using the dynamically coated capillary by capillary zone electrophoresis. Nine kinds of anions were separated and detected with the capillary. The electrophoretic mobility of the analyte anions decreased with increasing concentrations of chitosan in the migrating solution through ion-ion interaction, but the migration order of the analyte anions was not changed in the concentration range of the chitosan examined. The signal shape for the analyte anions was developed by using field-enhanced sample stacking with 10 mM sodium sulfate.

Correlation of refrigerant mass flow rate through adiabatic capillary tubes using mixture refrigerant carbondioxide and ethane for low temperature applications

NASA Astrophysics Data System (ADS)

Nasruddin, Syaka, Darwin R. B.; Alhamid, M. Idrus

2012-06-01

Various binary mixtures of carbon dioxide and hydrocarbons, especially propane or ethane, as alternative natural refrigerants to Chlorofluorocarbons (CFCs) or Hydro fluorocarbons (HFCs) are presented in this paper. Their environmental performance is friendly, with an ozone depletion potential (ODP) of zero and Global-warming potential (GWP) smaller than 20. The capillary tube performance for the alternative refrigerant HFC HCand mixed refrigerants have been widely studied. However, studies that discuss the performance of the capillary tube to a mixture of natural refrigerants, in particular a mixture of azeotrope carbon dioxide and ethane is still undeveloped. A method of empirical correlation to determine the mass flow rate and pipe length has an important role in the design of the capillary tube for industrial refrigeration. Based on the variables that effect the rate of mass flow of refrigerant in the capillary tube, the Buckingham Pi theorem formulated eight non-dimensional parameters to be developed into an empirical equations correlation. Furthermore, non-linear regression analysis used to determine the co-efficiency and exponent of this empirical correlation based on experimental verification of the results database.

High quantum yield ZnO quantum dots synthesizing via an ultrasonication microreactor method.

PubMed

Yang, Weimin; Yang, Huafang; Ding, Wenhao; Zhang, Bing; Zhang, Le; Wang, Lixi; Yu, Mingxun; Zhang, Qitu

2016-11-01

Green emission ZnO quantum dots were synthesized by an ultrasonic microreactor. Ultrasonic radiation brought bubbles through ultrasonic cavitation. These bubbles built microreactor inside the microreactor. The photoluminescence properties of ZnO quantum dots synthesized with different flow rate, ultrasonic power and temperature were discussed. Flow rate, ultrasonic power and temperature would influence the type and quantity of defects in ZnO quantum dots. The sizes of ZnO quantum dots would be controlled by those conditions as well. Flow rate affected the reaction time. With the increasing of flow rate, the sizes of ZnO quantum dots decreased and the quantum yields first increased then decreased. Ultrasonic power changed the ultrasonic cavitation intensity, which affected the reaction energy and the separation of the solution. With the increasing of ultrasonic power, sizes of ZnO quantum dots first decreased then increased, while the quantum yields kept increasing. The effect of ultrasonic temperature on the photoluminescence properties of ZnO quantum dots was influenced by the flow rate. Different flow rate related to opposite changing trend. Moreover, the quantum yields of ZnO QDs synthesized by ultrasonic microreactor could reach 64.7%, which is higher than those synthesized only under ultrasonic radiation or only by microreactor. Copyright © 2016 Elsevier B.V. All rights reserved.

Ultrasonic effect on the bubble nucleation and heat transfer of oscillating nanofluid

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhao, Nannan; Fu, Benwei; Key Laboratory of Marine, Mechanical and Manufacturing Engineering of the Ministry of Transport, Dalian 116026

Ultrasonic sound effect on bubble nucleation, oscillating motion activated by bubble formation, and its heat transfer enhancement of nanofluid was experimentally investigated. Nanofluid consists of distilled water and dysprosium (III) oxide (Dy{sub 2}O{sub 3}) nanoparticles with an average size of 98 nm and a mass ratio of 0.5%. Visualization results demonstrate that when the nanoparticles are added in the fluid influenced by the ultrasonic sound, bubble nucleation can be significantly enhanced. The oscillating motion initiated by the bubble formation of nanofluid under the influence of ultrasonic sound can significantly enhance heat transfer of nanofluid in an interconnected capillary loop.

Apparatus and method for ultrasonic treatment of a liquid

DOEpatents

Chandler, Darrell P.; Posakony, Gerald J.; Bond, Leonard J.; Bruckner-Lea, Cynthia J.

2006-04-04

The present invention is an apparatus for ultrasonically treating a liquid to generate a product. The apparatus is capable of treating a continuously-flowing, or intermittently-flowing, liquid along a line segment coincident with the flow path of the liquid. The apparatus has one or more ultrasonic transducers positioned asymmetrically about the line segment. The ultrasonic field encompasses the line segment and the ultrasonic energy may be concentrated along the line segment. Lysing treatments have been successfully achieved with efficiencies of greater than 99% using ultrasound at MHz frequencies without erosion or heating problems and without the need for chemical or mechanical pretreatment, or contrast agents. The present invention overcomes drawbacks of current ultrasonic treatments beyond lysing and opens up new sonochemical and sonophysical processing opportunities.

Method and apparatus to characterize ultrasonically reflective contrast agents

NASA Technical Reports Server (NTRS)

Pretlow, Robert A., III (Inventor)

1993-01-01

A method and apparatus for characterizing the time and frequency response of an ultrasonically reflective contrast agent is disclosed. An ultrasonically reflective contrast agent is injected, under constant pressure, into a fluid flowing through a pump flow circuit. The fluid and the ultrasonically reflective contrast agent are uniformly mixed in a mixing chamber, and the uniform mixture is passed through a contrast agent chamber. The contrast agent chamber is acoustically and axially interposed between an ultrasonic transducer chamber and an acoustic isolation chamber. A pulse of ultrasonic energy is transmitted into the contrast agent chamber from the ultrasonic transducer chamber. An echo waveform is received from the ultrasonically reflective contrast agent, and it is analyzed to determine the time and frequency response of the ultrasonically reflective contrast agent.

The roles of cerebral blood flow, capillary transit time heterogeneity, and oxygen tension in brain oxygenation and metabolism

PubMed Central

Jespersen, Sune N; Østergaard, Leif

2012-01-01

Normal brain function depends critically on moment-to-moment regulation of oxygen supply by the bloodstream to meet changing metabolic needs. Neurovascular coupling, a range of mechanisms that converge on arterioles to adjust local cerebral blood flow (CBF), represents our current framework for understanding this regulation. We modeled the combined effects of CBF and capillary transit time heterogeneity (CTTH) on the maximum oxygen extraction fraction (OEFmax) and metabolic rate of oxygen that can biophysically be supported, for a given tissue oxygen tension. Red blood cell velocity recordings in rat brain support close hemodynamic–metabolic coupling by means of CBF and CTTH across a range of physiological conditions. The CTTH reduction improves tissue oxygenation by counteracting inherent reductions in OEFmax as CBF increases, and seemingly secures sufficient oxygenation during episodes of hyperemia resulting from cortical activation or hypoxemia. In hypoperfusion and states of blocked CBF, both lower oxygen tension and CTTH may secure tissue oxygenation. Our model predicts that disturbed capillary flows may cause a condition of malignant CTTH, in which states of higher CBF display lower oxygen availability. We propose that conditions with altered capillary morphology, such as amyloid, diabetic or hypertensive microangiopathy, and ischemia–reperfusion, may disturb CTTH and thereby flow-metabolism coupling and cerebral oxygen metabolism. PMID:22044867

Cerebral capillary velocimetry based on temporal OCT speckle contrast.

PubMed

Choi, Woo June; Li, Yuandong; Qin, Wan; Wang, Ruikang K

2016-12-01

We propose a new optical coherence tomography (OCT) based method to measure red blood cell (RBC) velocities of single capillaries in the cortex of rodent brain. This OCT capillary velocimetry exploits quantitative laser speckle contrast analysis to estimate speckle decorrelation rate from the measured temporal OCT speckle signals, which is related to microcirculatory flow velocity. We hypothesize that OCT signal due to sub-surface capillary flow can be treated as the speckle signal in the single scattering regime and thus its time scale of speckle fluctuations can be subjected to single scattering laser speckle contrast analysis to derive characteristic decorrelation time. To validate this hypothesis, OCT measurements are conducted on a single capillary flow phantom operating at preset velocities, in which M-mode B-frames are acquired using a high-speed OCT system. Analysis is then performed on the time-varying OCT signals extracted at the capillary flow, exhibiting a typical inverse relationship between the estimated decorrelation time and absolute RBC velocity, which is then used to deduce the capillary velocities. We apply the method to in vivo measurements of mouse brain, demonstrating that the proposed approach provides additional useful information in the quantitative assessment of capillary hemodynamics, complementary to that of OCT angiography.

Capillary Channel Flow (CCF) EU2-02 on the International Space Station (ISS): An Experimental Investigation of Passive Bubble Separations in an Open Capillary Channel

NASA Technical Reports Server (NTRS)

Weislogel, Mark M.; Wollman, Andrew P.; Jenson, Ryan M.; Geile, John T.; Tucker, John F.; Wiles, Brentley M.; Trattner, Andy L.; DeVoe, Claire; Sharp, Lauren M.; Canfield, Peter J.;

Flow profiling of a surface-acoustic-wave nanopump.

PubMed

Guttenberg, Z; Rathgeber, A; Keller, S; Rädler, J O; Wixforth, A; Kostur, M; Schindler, M; Talkner, P

2004-11-01

The flow profile in a capillary gap and the pumping efficiency of an acoustic micropump employing surface acoustic waves is investigated both experimentally and theoretically. Ultrasonic surface waves on a piezoelectric substrate strongly couple to a thin liquid layer and generate a quadrupolar streaming pattern within the fluid. We use fluorescence correlation spectroscopy and fluorescence microscopy as complementary tools to investigate the resulting flow profile. The velocity was found to depend on the applied power approximately linearly and to decrease with the inverse third power of the distance from the ultrasound generator on the chip. The found properties reveal acoustic streaming as a promising tool for the controlled agitation during microarray hybridization.

Flow profiling of a surface-acoustic-wave nanopump

NASA Astrophysics Data System (ADS)

Guttenberg, Z.; Rathgeber, A.; Keller, S.; Rädler, J. O.; Wixforth, A.; Kostur, M.; Schindler, M.; Talkner, P.

2004-11-01

The flow profile in a capillary gap and the pumping efficiency of an acoustic micropump employing surface acoustic waves is investigated both experimentally and theoretically. Ultrasonic surface waves on a piezoelectric substrate strongly couple to a thin liquid layer and generate a quadrupolar streaming pattern within the fluid. We use fluorescence correlation spectroscopy and fluorescence microscopy as complementary tools to investigate the resulting flow profile. The velocity was found to depend on the applied power approximately linearly and to decrease with the inverse third power of the distance from the ultrasound generator on the chip. The found properties reveal acoustic streaming as a promising tool for the controlled agitation during microarray hybridization.

Apparatus and method for ultrasonic treatment of a liquid

DOEpatents

Chandler, Darrell P [Richland, WA; Posakony, Gerald J [Richland, WA; Bond, Leonard J [Richland, WA; Bruckner-Lea, Cynthia J [Richland, WA

2003-01-14

The present invention is an apparatus and method for ultrasonically treating a liquid to generate a product. The apparatus is capable of treating a continuously-flowing, or intermittently-flowing, liquid along a line segment coincident with the flow path of the liquid. The apparatus has one or more ultrasonic transducers positioned asymmetrically about the line segment. The ultrasonic field encompasses the line segment and the ultrasonic energy may be concentrated along the line segment. Lysing treatments have been successfully achieved with efficiencies of greater than 99% using ultrasound at MHz frequencies without erosion or heating problems and without the need for chemical or mechanical pretreatment, or contrast agents. The present invention overcomes drawbacks of current ultrasonic treatments beyond lysing and opens up new sonochemical and sonophysical processing opportunities.

Study on Gap Flow Field Simulation in Small Hole Machining of Ultrasonic Assisted EDM

NASA Astrophysics Data System (ADS)

Liu, Yu; Chang, Hao; Zhang, Wenchao; Ma, Fujian; Sha, Zhihua; Zhang, Shengfang

2017-12-01

When machining a small hole with high aspect ratio in EDM, it is hard for the flushing liquid entering the bottom gap and the debris could hardly be removed, which results in the accumulation of debris and affects the machining efficiency and machining accuracy. The assisted ultrasonic vibration can improve the removal of debris in the gap. Based on dynamics simulation software Fluent, a 3D model of debris movement in the gap flow field of EDM small hole machining assisted with side flushing and ultrasonic vibration is established in this paper. When depth to ratio is 3, the laws of different amplitudes and frequencies on debris distribution and removal are quantitatively analysed. The research results show that periodic ultrasonic vibration can promote the movement of debris, which is beneficial to the removal of debris in the machining gap. Compared to traditional small hole machining in EDM, the debris in the machining gap is greatly reduced, which ensures the stability of machining process and improves the machining efficiency.

Optical coherence tomography angiography-based capillary velocimetry

NASA Astrophysics Data System (ADS)

Wang, Ruikang K.; Zhang, Qinqin; Li, Yuandong; Song, Shaozhen

2017-06-01

Challenge persists in the field of optical coherence tomography (OCT) when it is required to quantify capillary blood flow within tissue beds in vivo. We propose a useful approach to statistically estimate the mean capillary flow velocity using a model-based statistical method of eigendecomposition (ED) analysis of the complex OCT signals obtained with the OCT angiography (OCTA) scanning protocol. ED-based analysis is achieved by the covariance matrix of the ensemble complex OCT signals, upon which the eigenvalues and eigenvectors that represent the subsets of the signal makeup are calculated. From this analysis, the signals due to moving particles can be isolated by employing an adaptive regression filter to remove the eigencomponents that represent static tissue signals. The mean frequency (MF) of moving particles can be estimated by the first lag-one autocorrelation of the corresponding eigenvectors. Three important parameters are introduced, including the blood flow signal power representing the presence of blood flow (i.e., OCTA signals), the MF indicating the mean velocity of blood flow, and the frequency bandwidth describing the temporal flow heterogeneity within a scanned tissue volume. The proposed approach is tested using scattering phantoms, in which microfluidic channels are used to simulate the functional capillary vessels that are perfused with the scattering intralipid solution. The results indicate a linear relationship between the MF and mean flow velocity. In vivo animal experiments are also conducted by imaging mouse brain with distal middle cerebral artery ligation to test the capability of the method to image the changes in capillary flows in response to an ischemic insult, demonstrating the practical usefulness of the proposed method for providing important quantifiable information about capillary tissue beds in the investigations of neurological conditions in vivo.

The development of recent high-power ultrasonic transducers for Near-well ultrasonic processing technology.

PubMed

Wang, Zhenjun; Xu, Yuanming

2017-07-01

With the reduction of crude oil throughout the world, enhance oil recovery technology has become a major oil research topics, which can greatly increase the recovery ratio of the crude oil before the dawning of renewable energy era. Near-well ultrasonic processing technology, as one new method, has attracted more attention for Enhanced Oil Recovery due to its low cost, good applicability and no environmental pollution in recent rears. There are two important relevant aspects about Near-well ultrasonic processing technology: (a) how to enhance the oil flow through the rocks into the pumping pool and (b) how to reduce the oil viscosity so that it can be easier to pump. Therefore, how to design a high-power ultrasonic equipment with excellent performance is crucial for Near-well ultrasonic processing technology. In this paper, recent new high-power ultrasonic transducers for Near-well ultrasonic processing technology are summarized. Each field application of them are also given. The purpose of this paper is to provide reference for the further development of Near-well ultrasonic processing technology. With the reduction of crude oil throughout the world, enhance oil recovery technology has become a major oil research topics, which can greatly increase the recovery ratio of the crude oil before the dawning of renewable energy era. Near-well ultrasonic processing technology, as one new method, has attracted more attention for Enhanced Oil Recovery due to its low cost, good applicability and no environmental pollution in recent rears. There are two important relevant aspects about Near-well ultrasonic processing technology: (a) how to enhance the oil flow through the rocks into the pumping pool and (b) how to reduce the oil viscosity so that it can be easier to pump. Therefore, how to design a high-power ultrasonic equipment with excellent performance is crucial for Near-well ultrasonic processing technology. In this paper, recent new high-power ultrasonic transducers

Influence of Thermocapillary Flow on Capillary Stability: Long Float-Zones in Low Gravity

NASA Technical Reports Server (NTRS)

Chen, Yi-Ju; Steen, Paul H.

1996-01-01

A model problem is posed to study the influence of flow on the interfacial stability of a nearly cylindrical liquid bridge for lengths near its circumference (the Plateau-Rayleigh limit). The flow is generated by a shear stress imposed on the deformable interface. The symmetry of the imposed shear stress mimics the thermocapillary stress induced on a float-zone by a ring heater (i.e. a full zone). Principal assumptions are (1) zero gravity, (2) creeping flow, and (3) that the imposed coupling at the free surface between flow and temperature fields is the only such coupling. A numerical solution, complemented by a bifurcation analysis, shows that bridges substantially longer than the Plateau-Rayleigh limit are possible. An interaction of the first two capillary instabilities through the stress-induced flow is responsible. Time-periodic standing waves are also predicted in certain parameter ranges. Motivation comes from extra-long float-zones observed in MEPHISTO space lab experiments (June 1994).

Comprehensive protein profiling by multiplexed capillary zone electrophoresis using cross-linked polyacrylamide coated capillaries.

PubMed

Liu, Shaorong; Gao, Lin; Pu, Qiaosheng; Lu, Joann J; Wang, Xingjia

2006-02-01

We have recently developed a new process to create cross-linked polyacrylamide (CPA) coatings on capillary walls to suppress protein-wall interactions. Here, we demonstrate CPA-coated capillaries for high-efficiency (>2 x 10(6) plates per meter) protein separations by capillary zone electrophoresis (CZE). Because CPA virtually eliminates electroosmotic flow, positive and negative proteins cannot be analyzed in a single run. A "one-sample-two-separation" approach is developed to achieve a comprehensive protein analysis. High throughput is achieved through a multiplexed CZE system.

Nanoelectrospray ion generation for high-throughput mass spectrometry using a micromachined ultrasonic ejector array

DOE Office of Scientific and Technical Information (OSTI.GOV)

Aderogba, S.; Meacham, J.M.; Degertekin, F.L.

2005-05-16

Ultrasonic electrospray ionization (ESI) for high-throughput mass spectrometry is demonstrated using a silicon micromachined microarray. The device uses a micromachined ultrasonic atomizer operating in the 900 kHz-2.5 MHz range for droplet generation and a metal electrode in the fluid cavity for ionization. Since the atomization and ionization processes are separated, the ultrasonic ESI source shows the potential for operation at low voltages with a wide range of solvents in contrast with conventional capillary ESI technology. This is demonstrated using the ultrasonic ESI microarray to obtain the mass spectrum of a 10 {mu}M reserpine sample on a time of flight massmore » spectrometer with 197:1 signal-to-noise ratio at an ionization potential of 200 V.« less

Sol-gel open tubular ODS columns with reversed electroosmotic flow for capillary electrochromatography.

PubMed

Hayes, J D; Malik, A

2001-03-01

Sol-gel chemistry was successfully used for the fabrication of open tubular columns with surface-bonded octadecylsilane (ODS) stationary-phase coating for capillary electrochromatography (OT-CEC). Following column preparations, a series of experiments were performed to investigate the performance of the sol-gel coated ODS columns in OT-CEC. The incorporation of N-octadecyldimethyl[3-(trimethoxysilyl)propyl]ammonium chloride as one of the sol-gel precursors played an important role in the electrochromatographic performance of the prepared columns. This chemical reagent possesses a chromatographically favorable, bonded ODS moiety, in conjunction with three methoxy groups allowing for sol-gel reactivity. In addition, a positively charged nitrogen atom is present in the molecular structure of this reagent and provides a positively charged capillary surface responsible for the reversed electroosmotic flow (EOF) in the columns during CEC operation. Comparative studies involving the EOF within such sol-gel ODS coated and uncoated capillaries were performed using acetonitrile and methanol as the organic modifiers in the mobile phase. The use of a deactivating reagent, phenyldimethylsilane, in the sol-gel solution was evaluated. Efficiency values of over 400,000 theoretical plates per meter were achieved in CEC on a 64 cm x 25 microm i.d. sol-gel ODS open tubular column. Test mixtures of polycyclic aromatic hydrocarbons, benzene derivatives, and aromatic aldehydes and ketones were used to evaluate the CEC performances of both nondeactivated and deactivated open tubular sol-gel columns. The effects of mobile-phase organic modifier contents and pH on EOF in such columns were evaluated. The prepared sol-gel ODS columns are characterized by switchable electroosmotic flow. A pH value of approximately 8.5 was found correspond to the isoelectric point for the prepared sol-gel ODS coatings.

Ultrasonic drilling apparatus

DOEpatents

Duran, Edward L.; Lundin, Ralph L.

1989-01-01

Apparatus attachable to an ultrasonic drilling machine for drilling deep holes in very hard materials, such as boron carbide, is provided. The apparatus utilizes a hollow spindle attached to the output horn of the ultrasonic drilling machine. The spindle has a hollow drill bit attached at the opposite end. A housing surrounds the spindle, forming a cavity for holding slurry. In operation, slurry is provided into the housing, and into the spindle through inlets while the spindle is rotating and ultrasonically reciprocating. Slurry flows through the spindle and through the hollow drill bit to cleanse the cutting edge of the bit during a drilling operation.

Ultrasonic drilling apparatus

DOEpatents

Duran, E.L.; Lundin, R.L.

1988-06-20

Apparatus attachable to an ultrasonic drilling machine for drilling deep holes in very hard materials, such as boron carbide, is provided. The apparatus utilizes a hollow spindle attached to the output horn of the ultrasonic drilling machine. The spindle has a hollow drill bit attached at the opposite end. A housing surrounds the spindle, forming a cavity for holding slurry. In operation, slurry is provided into the housing, and into the spindle through inlets while the spindle is rotating and ultrasonically reciprocating. Slurry flows through the spindle and through the hollow drill bit to cleanse the cutting edge of the bit during a drilling operation. 3 figs.

Aggregation behavior of fullerenes in aqueous solutions: a capillary electrophoresis and asymmetric flow field-flow fractionation study.

PubMed

Astefanei, Alina; Núñez, Oscar; Galceran, Maria Teresa; Kok, Wim Th; Schoenmakers, Peter J

2015-10-01

In this work, the electrophoretic behavior of hydrophobic fullerenes [buckminsterfullerene (C60), C70, and N-methyl-fulleropyrrolidine (C60-pyrr)] and water-soluble fullerenes [fullerol (C60(OH)24); polyhydroxy small gap fullerene, hydrated (C120(OH)30); C60 pyrrolidine tris acid (C60-pyrr tris acid); and (1,2-methanofullerene C60)-61-carboxylic acid (C60CHCOOH)] in micellar electrokinetic capillary chromatography (MECC) was evaluated. The aggregation behavior of the water-soluble compounds in MECC at different buffer and sodium dodecyl sulfate (SDS) concentrations and pH values of the background electrolyte (BGE) was studied by monitoring the changes observed in the electrophoretic pattern of the peaks. Broad and distorted peaks that can be attributed to fullerene aggregation were obtained in MECC which became narrower and more symmetric by working at low buffer and SDS concentrations (below the critical micelle concentration, capillary zone electrophoresis (CZE) conditions). For the characterization of the suspected aggregates formed (size and shape), asymmetrical flow field-flow fractionation (AF4) and transmission electron microscopy (TEM) were used. The results showed that the increase in the buffer concentration promoted the aggregation of the particles, while the presence of SDS micelles revealed multiple peaks corresponding to particles of different aggregation degrees. Furthermore, MECC has been applied for the first time for the analysis of C60 in two different cosmetic products (i.e., anti-aging serum and facial mask).

Laws of physics help explain capillary non-perfusion in diabetic retinopathy.

PubMed

Stefánsson, E; Chan, Y K; Bek, T; Hardarson, S H; Wong, D; Wilson, D I

2018-02-01

The purpose is to use laws of physics to elucidate the mechanisms behind capillary non-perfusion in diabetic retinopathy. In diabetic retinopathy, loss of pericytes weakens capillary walls and the vessel dilates. A dilated capillary has reduced resistance to flow, therefore increased flow in that vessel and decreased in adjoining capillaries. A preferential shunt vessel is thus formed from the dilated capillary and the adjacent capillaries become non-perfused. We apply the laws of Laplace and Hagen-Poiseuille to better understand the phenomena that lead to capillary non-perfusion. These laws of physics can give a foundation for physical or mathematical models to further elucidate this field of study. The law of Laplace predicts that a weaker vessel wall will dilate, assuming constant transmural pressure. The Hagen-Poiseuille equation for flow and the Ostwald-de Waele relationship for viscosity predict that a dilated vessel will receive a higher portion of the fluid flow than the adjoining capillaries. Viscosity will decrease in the dilated vessel, furthering the imbalance and resulting in a patch of non-perfused capillaries next to the dilated 'preferential' shunt vessel. Physical principles support or inspire novel hypotheses to explain poorly understood phenomena in ophthalmology. This thesis of pericyte death and capillary remodelling, which was first proposed by Cogan and Kuwabara, already agrees with histological and angiographical observations in diabetic retinopathy. We have shown that it is also supported by classical laws of physics.

Ultrasonic Mastering of Filter Flow and Antifouling of Renewable Resources.

PubMed

Radziuk, Darya; Möhwald, Helmuth

2016-04-04

Inadequate access to pure water and sanitation requires new cost-effective, ergonomic methods with less consumption of energy and chemicals, leaving the environment cleaner and sustainable. Among such methods, ultrasound is a unique means to control the physics and chemistry of complex fluids (wastewater) with excellent performance regarding mass transfer, cleaning, and disinfection. In membrane filtration processes, it overcomes diffusion limits and can accelerate the fluid flow towards the filter preventing antifouling. Here, we outline the current state of knowledge and technological design, with a focus on physicochemical strategies of ultrasound for water cleaning. We highlight important parameters of ultrasound for the delivery of a fluid flow from a technical perspective employing principles of physics and chemistry. By introducing various ultrasonic methods, involving bubbles or cavitation in combination with external fields, we show advancements in flow acceleration and mass transportation to the filter. In most cases we emphasize the main role of streaming and the impact of cavitation with a perspective to prevent and remove fouling deposits during the flow. We also elaborate on the deficiencies of present technologies and on problems to be solved to achieve a wide-spread application. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Dynamic stability analysis for capillary channel flow: One-dimensional and three-dimensional computations and the equivalent steady state technique

NASA Astrophysics Data System (ADS)

Grah, Aleksander; Dreyer, Michael E.

2010-01-01

Spacecraft technology provides a series of applications for capillary channel flow. It can serve as a reliable means for positioning and transport of liquids under low gravity conditions. Basically, capillary channels provide liquid paths with one or more free surfaces. A problem may be flow instabilities leading to a collapse of the liquid surfaces. A result is undesired gas ingestion and a two phase flow which can in consequence cause several technical problems. The presented capillary channel consists of parallel plates with two free liquid surfaces. The flow rate is established by a pump at the channel outlet, creating a lower pressure within the channel. Owing to the pressure difference between the liquid phase and the ambient gas phase the free surfaces bend inwards and remain stable as long as they are able to resist the steady and unsteady pressure effects. For the numerical prediction of the flow stability two very different models are used. The one-dimensional unsteady model is mainly based on the Bernoulli equation, the continuity equation, and the Gauss-Laplace equation. For three-dimensional evaluations an open source computational fluid dynamics (CFD) tool is applied. For verifications the numerical results are compared with quasisteady and unsteady data of a sounding rocket experiment. Contrary to previous experiments this one results in a significantly longer observation sequence. Furthermore, the critical point of the steady flow instability could be approached by a quasisteady technique. As in previous experiments the comparison to the numerical model evaluation shows a very good agreement for the movement of the liquid surfaces and for the predicted flow instability. The theoretical prediction of the flow instability is related to the speed index, based on characteristic velocities of the capillary channel flow. Stable flow regimes are defined by stability criteria for steady and unsteady flow. The one-dimensional computation of the speed index

Retention behavior of neutral solutes in pressurized flow-driven capillary electrochromatography using an ODS column.

PubMed

Nakagawa, Hiroyuki; Kitagawa, Shinya; Araki, Shuki; Ohtani, Hajime

2006-02-01

Several alkyl benzenes are separated by pressurized flow-driven capillary electrochromatography using a temperature-controlled capillary column packed with octadecyl siloxane-modified silica gel, and the effect of applied voltage on the retention is investigated. The van't Hoff plot shows good linearity at the column temperature between 305 and 330 K under applications from -6 to +6 kV. The applied voltage causes a relatively large variation in the enthalpy and the entropy of transfer of the solute from the mobile phase to the stationary phase (> 20%). However, the direction of variation in the enthalpy is almost opposite to that in the entropy, both of which might compensate each other. Therefore, the retention factor is not significantly varied (< 4%) by the application of voltage.

Investigation of the Hydraulic Characteristics of Capillary Elements of the Injector Head of Jet Engines under Conditions of Isothermal Flow of A Liquid

NASA Astrophysics Data System (ADS)

Nigodjuk, V. E.; Sulinov, A. V.

2018-01-01

The article presents the results of an experimental study of the hydraulic characteristics of capillary elements of the injector head of jet engines in isothermal fluid flow and the proposed method of their calculation. The main geometric dimensions of the capillaries in the experiment were changed in the following range: Inner diameter from 0.16 to 0.36 mm, length from 4.3 to 158 mm and relative length from 25 to 614 and the inlet edge of the capillaries: sharp or smooth the leading edge. As the working fluid during the tests were distilled water, acetone and ethyl alcohol. Based on the results of a study of the dependences for calculation of ultimate losses in laminar and turbulent flow regimes in capillary tubes with smooth and sharp edges input. The influence of surface tension forces on loss of input on a sharp cutting edge. Experimentally confirmed the possibility of calculating the linear coefficient of hydraulic resistance of capillary tubes with a diameter of 0.16-0.36 mm in isothermal stable during the known dependencies that are valid for hydrodynamically smooth round tube.

Fragmentation mechanisms of confined co-flowing capillary threads revealed by active flow focusing

NASA Astrophysics Data System (ADS)

Robert de Saint Vincent, Matthieu; Delville, Jean-Pierre

2016-08-01

The control over stationary liquid thread fragmentation in confined co-flows is a key issue for the processing and transport of fluids in (micro-)ducts. Confinement indeed strongly enhances the stability of capillary threads, and also induces steric and hydrodynamic feedback effects on diphasic flows. We investigate the thread-to-droplet transition within the confined environment of a microchannel by using optocapillarity, i.e., interface stresses driven by light, as a wall-free constriction to locally flow focus stable threads in a tunable way, pinch them, and force their fragmentation. Above some flow-dependent onset in optical forcing, we observe a dynamic transition alternating between continuous (thread) and fragmented (droplets) states and show a surprisingly gradual thread-to-droplet transition when increasing the amplitude of the thread constriction. This transition is interpreted as an evolution from a convective to an absolute instability. Depending on the forcing amplitude, we then identify and characterize several stable fragmented regimes of single and multiple droplet periodicity (up to period-8). These droplet regimes build a robust flow-independent bifurcation diagram that eventually closes up, due to the flow confinement, to a monodisperse droplet size, independent of the forcing and close to the most unstable mode expected from the Rayleigh-Plateau instability. This fixed monodispersity can be circumvented by temporally modulating the optocapillary coupling, as we show that fragmentation can then occur either by triggering again the Rayleigh-Plateau instability when the largest excitable wavelength is larger than that of the most unstable mode, or as a pure consequence of a sufficiently strong optocapillary pinching. When properly adjusted, this modulation allows us to avoid the transient reforming and multidisperse regimes, and thereby to reversibly produce stable monodisperse droplet trains of controlled size. By actuating local flow focusing in

Ultrasonic sensing for noninvasive characterization of oil-water-gas flow in a pipe

NASA Astrophysics Data System (ADS)

Chillara, Vamshi Krishna; Sturtevant, Blake T.; Pantea, Cristian; Sinha, Dipen N.

2017-02-01

A technique for noninvasive ultrasonic characterization of multiphase crude oil-water-gas flow is discussed. The proposed method relies on determining the sound speed in the mixture. First, important issues associated with making real-time noninvasive measurements are discussed. Then, signal processing approach adopted to determine the sound speed in the multiphase mixture is presented. Finally, results from controlled experiments on crude oil-water mixture in both the presence and absence of gas are presented.

Integrated multiplexed capillary electrophoresis system

DOEpatents

Yeung, Edward S.; Tan, Hongdong

2002-05-14

The present invention provides an integrated multiplexed capillary electrophoresis system for the analysis of sample analytes. The system integrates and automates multiple components, such as chromatographic columns and separation capillaries, and further provides a detector for the detection of analytes eluting from the separation capillaries. The system employs multiplexed freeze/thaw valves to manage fluid flow and sample movement. The system is computer controlled and is capable of processing samples through reaction, purification, denaturation, pre-concentration, injection, separation and detection in parallel fashion. Methods employing the system of the invention are also provided.

[A capillary blood flow velocity detection system based on linear array charge-coupled devices].

PubMed

Zhou, Houming; Wang, Ruofeng; Dang, Qi; Yang, Li; Wang, Xiang

2017-12-01

In order to detect the flow characteristics of blood samples in the capillary, this paper introduces a blood flow velocity measurement system based on field-programmable gate array (FPGA), linear charge-coupled devices (CCD) and personal computer (PC) software structure. Based on the analysis of the TCD1703C and AD9826 device data sheets, Verilog HDL hardware description language was used to design and simulate the driver. Image signal acquisition and the extraction of the real-time edge information of the blood sample were carried out synchronously in the FPGA. Then a series of discrete displacement were performed in a differential operation to scan each of the blood samples displacement, so that the sample flow rate could be obtained. Finally, the feasibility of the blood flow velocity detection system was verified by simulation and debugging. After drawing the flow velocity curve and analyzing the velocity characteristics, the significance of measuring blood flow velocity is analyzed. The results show that the measurement of the system is less time-consuming and less complex than other flow rate monitoring schemes.

Revealing the physicochemical mechanism for ultrasonic separation of alcohol-water mixtures

NASA Astrophysics Data System (ADS)

Kirpalani, D. M.; Toll, F.

2002-08-01

The selective separation of ethanol from ethanol-water mixtures by ultrasonic atomization has been reported recently by Sato, Matsuura, and Fujii [J. Chem. Phys. 114, 2382 (2001)]. In that work, experimental data were reported that confirmed the generation of an ethanol-rich droplet mist and attempted to explain the selective separation in terms of parametric decay instability of the capillary wave formed during sonication. In the present work, an alternate mechanism based on the conjunction theory has been postulated for the process of ultrasonic atomization. This mechanism involves the formation of cavitating bubbles in the liquid during sonication and their eventual collapse at the liquid surface into a cloud of microbubbles that moves upwards in a capillary fountain jet. The selective separation of alcohols has been explained as a corollary effect of the physical mechanism resulting in a surface excess of alcohol molecules formed at the surface of the microbubbles. The alcohol molecules vaporize into the microbubbles and release an alcohol-rich mist on their collapse in regions of high accumulation of acoustic energy.

Capillary toroid cavity detector for high pressure NMR

DOEpatents

Gerald, II, Rex E.; Chen, Michael J.; Klingler, Robert J.; Rathke, Jerome W.; ter Horst, Marc

2007-09-11

A Toroid Cavity Detector (TCD) is provided for implementing nuclear magnetic resonance (NMR) studies of chemical reactions under conditions of high pressures and temperatures. A toroid cavity contains an elongated central conductor extending within the toroid cavity. The toroid cavity and central conductor generate an RF magnetic field for NMR analysis. A flow-through capillary sample container is located within the toroid cavity adjacent to the central conductor to subject a sample material flowing through the capillary to a static magnetic field and to enable NMR spectra to be recorded of the material in the capillary under a temperature and high pressure environment.

Plastic Deformation Behavior of Ti Foil Under Ultrasonic Vibration in Tension

NASA Astrophysics Data System (ADS)

Jiang, Shaosong; Jia, Yong; Zhang, Hongbin; Du, Zhihao; Lu, Zhen; Zhang, Kaifeng; He, Yushi; Wang, Ruizhuo

2017-04-01

The benefits of ultrasonic vibration auxiliary metal forming have been shown by many studies. In this study, a series of experiments were carried out to investigate the deformation behavior of Ti foils under ultrasonic vibration in tension, and the tensile properties of Ti foils with/without the application of ultrasonic vibration were investigated. Then, the microstructure of different tensile samples was analyzed by transmission electron microscopy (TEM). The results of the tensile experiments showed that the tensile strength of tensile samples was reduced when ultrasonic vibration was applied, while the elongation of these samples increased. The flow stress increased with increasing strain without applying ultrasonic vibration, while it decreased steeply when the ultrasonic vibration was applied, and this reduction of flow stress demonstrated the effect of acoustic softening on the properties of the material. Additionally, the range of flow stress reduction was inversely proportional to the time for which ultrasonic vibration was applied. The TEM images showed that there were remarkable differences in dislocation distribution and tangles with/without ultrasonic vibration. The dislocation distribution was inhomogeneous, and copious dislocation tangles were discovered without ultrasonic vibration. When it was applied, the parallel re-arrangement of dislocations could be observed and the mass of dislocation tangles was mostly absent.

Ultrasonic filtration of industrial chemical solutions

NASA Technical Reports Server (NTRS)

Cosma, T.

1974-01-01

The practical results obtained as a result of filtering industrial chemical solutions under continuous flow conditions with the aid of an ultrasonic filter are presented. The main part of the assembly consists of an ultrasonic generator with an output power of about 400 W and the filtration assembly, in which there is a magnetostrictive amplifier constructed for 20.5 kHz. In addition to ensuring a continuous flow of filtered solution, ultrasonic filters can be replaced or cleaned at intervals of time that are 8-10 times greater than in the case of mechanical filters. They yield considerably better results as far as the size of the filtered particles is concerned. The parameters on which filtration quality depends are also presented.

Measurement of lung volume in mechanically ventilated monkeys with an ultrasonic flow meter and the nitrogen washout method.

PubMed

Schibler, Andreas; Hammer, Jürg; Isler, Ruedi; Buess, Christian; Newth, Christopher J L

2004-01-01

Measurement of functional residual capacity (FRC) during mechanical ventilation is important to standardise respiratory system compliance and adjust the ventilator settings to optimise lung recruitment. In the present study we compared three methods to measure FRC. The bias flow nitrogen washout technique (FRC(N2MC)), the multiple breath nitrogen washout (FRC(MBNW)) and the multiple breath sulphur-hexafluoride washout using the molar mass signal of an ultrasonic flow meter (FRC(MBSF6)) were compared in six adult monkeys after endotracheal intubation and during spontaneous breathing and mechanical ventilation at three different positive end-expiratory pressure (PEEP) levels of 0, 5 and 10 cmH2O. Animal research laboratory. We found good agreement between all three methods and they all accurately measured changes in FRC when PEEP was increased. The coefficients of variance of the three measurement techniques were in the same range (1.3-9.2%). The measurement of the tracer gas concentration with the molar mass signal of the ultrasonic flow meter provides a good and simple alternative to respiratory mass spectrometer for FRC measurements in ventilated subjects.

CFD study of the flow pattern in an ultrasonic horn reactor: Introducing a realistic vibrating boundary condition.

PubMed

Rahimi, Masoud; Movahedirad, Salman; Shahhosseini, Shahrokh

2017-03-01

Recently, great attention has been paid to predict the acoustic streaming field distribution inside the sonoreactors, induced by high-power ultrasonic wave generator. The focus of this paper is to model an ultrasonic vibrating horn and study the induced flow pattern with a newly developed moving boundary condition. The numerical simulation utilizes the modified cavitation model along with the "mixture" model for turbulent flow (RNG, k-ε), and a moving boundary condition with an oscillating parabolic-logarithmic profile, applied to the horn tip. This moving-boundary provides the situation in which the center of the horn tip vibrates stronger than that of the peripheral regions. The velocity field obtained by computational fluid dynamic was in a reasonably good agreement with the PIV results. The moving boundary model is more accurate since it better approximates the movement of the horn tip in the ultrasonic assisted process. From an optimizing point of view, the model with the new moving boundary is more suitable than the conventional models for design purposes because the displacement magnitude of the horn tip is the only fitting parameter. After developing and validating the numerical model, the model was utilized to predict various quantities such as cavitation zone, pressure field and stream function that are not experimentally feasible to measure. Copyright © 2016 Elsevier B.V. All rights reserved.

X-ray imaging of water motion during capillary imbibition: A study on how compaction bands impact fluid flow in Bentheim sandstone

NASA Astrophysics Data System (ADS)

Pons, A.; David, C.; Fortin, J.; Stanchits, S.; MenéNdez, B.; Mengus, J. M.

2011-03-01

To investigate the effect of compaction bands (CB) on fluid flow, capillary imbibition experiments were performed on Bentheim sandstone specimens (initial porosity ˜22.7%) using an industrial X-ray scanner. We used a three-step procedure combining (1) X-ray imaging of capillary rise in intact Bentheim sandstone, (2) formation of compaction band under triaxial tests, at 185 MPa effective pressure, with acoustic emissions (AE) recording for localization of the induced damage, and (3) again X-ray imaging of capillary rise in the damaged specimens after the unloading. The experiments were performed on intact cylindrical specimens, 5 cm in diameter and 10.5 cm in length, cored in different orientations (parallel or perpendicular to the bedding). Analysis of the images obtained at different stages of the capillary imbibition shows that the presence of CB slows down the imbibition and disturbs the geometry of water flow. In addition, we show that the CB geometry derived from X-ray density maps analysis is well correlated with the AE location obtained during triaxial test. The analysis of the water front kinetics was conducted using a simple theoretical model, which allowed us to confirm that compaction bands act as a barrier for fluid flow, not fully impermeable though. We estimate a contrast of permeability of a factor of ˜3 between the host rock and the compaction bands. This estimation of the permeability inside the compaction band is consistent with estimations done in similar sandstones from field studies but differs by 1 order of magnitude from estimations from previous laboratory measurements.

Depicting mass flow rate of R134a /LPG refrigerant through straight and helical coiled adiabatic capillary tubes of vapor compression refrigeration system using artificial neural network approach

NASA Astrophysics Data System (ADS)

Gill, Jatinder; Singh, Jagdev

2018-07-01

In this work, an experimental investigation is carried out with R134a and LPG refrigerant mixture for depicting mass flow rate through straight and helical coil adiabatic capillary tubes in a vapor compression refrigeration system. Various experiments were conducted under steady-state conditions, by changing capillary tube length, inner diameter, coil diameter and degree of subcooling. The results showed that mass flow rate through helical coil capillary tube was found lower than straight capillary tube by about 5-16%. Dimensionless correlation and Artificial Neural Network (ANN) models were developed to predict mass flow rate. It was found that dimensionless correlation and ANN model predictions agreed well with experimental results and brought out an absolute fraction of variance of 0.961 and 0.988, root mean square error of 0.489 and 0.275 and mean absolute percentage error of 4.75% and 2.31% respectively. The results suggested that ANN model shows better statistical prediction than dimensionless correlation model.

In situ x-ray diffraction measurements of the capillary fountain jet produced via ultrasonic atomization.

PubMed

Yano, Yohko F; Douguchi, Junya; Kumagai, Atsushi; Iijima, Takao; Tomida, Yukinobu; Miyamoto, Toshiaki; Matsuura, Kazuo

2006-11-07

In situ x-ray diffraction measurements were carried out for investigating the liquid structure in the ultrasonic fountain jet to consider the mechanism of the "ultrasonic ethanol separation" reported by Sato et al. [J. Chem. Phys. 114, 2382 (2001)]. For pure liquids (water and ethanol), it was found that the high frequency ultrasound does not affect the liquid structure microscopically. For the 20 mol % ethanol-water mixture, the estimated ethanol mole fraction in the ultrasonic fountain jet by using the position of the main maximum in the x-ray diffraction profile coincided with that in the reservoir. This result suggests that the ethanol separation is not caused by any distorted liquid structure under the ultrasound irradiation and occurs when or after the generation of the liquid droplet mist.

In situ x-ray diffraction measurements of the capillary fountain jet produced via ultrasonic atomization

NASA Astrophysics Data System (ADS)

Yano, Yohko F.; Douguchi, Junya; Kumagai, Atsushi; Iijima, Takao; Tomida, Yukinobu; Miyamoto, Toshiaki; Matsuura, Kazuo

2006-11-01

In situ x-ray diffraction measurements were carried out for investigating the liquid structure in the ultrasonic fountain jet to consider the mechanism of the "ultrasonic ethanol separation" reported by Sato et al. [J. Chem. Phys. 114, 2382 (2001)]. For pure liquids (water and ethanol), it was found that the high frequency ultrasound does not affect the liquid structure microscopically. For the 20mol% ethanol-water mixture, the estimated ethanol mole fraction in the ultrasonic fountain jet by using the position of the main maximum in the x-ray diffraction profile coincided with that in the reservoir. This result suggests that the ethanol separation is not caused by any distorted liquid structure under the ultrasound irradiation and occurs when or after the generation of the liquid droplet mist.

Soft Sensing of Non-Newtonian Fluid Flow in Open Venturi Channel Using an Array of Ultrasonic Level Sensors—AI Models and Their Validations

PubMed Central

Viumdal, Håkon; Mylvaganam, Saba

2017-01-01

In oil and gas and geothermal installations, open channels followed by sieves for removal of drill cuttings, are used to monitor the quality and quantity of the drilling fluids. Drilling fluid flow rate is difficult to measure due to the varying flow conditions (e.g., wavy, turbulent and irregular) and the presence of drilling cuttings and gas bubbles. Inclusion of a Venturi section in the open channel and an array of ultrasonic level sensors above it at locations in the vicinity of and above the Venturi constriction gives the varying levels of the drilling fluid in the channel. The time series of the levels from this array of ultrasonic level sensors are used to estimate the drilling fluid flow rate, which is compared with Coriolis meter measurements. Fuzzy logic, neural networks and support vector regression algorithms applied to the data from temporal and spatial ultrasonic level measurements of the drilling fluid in the open channel give estimates of its flow rate with sufficient reliability, repeatability and uncertainty, providing a novel soft sensing of an important process variable. Simulations, cross-validations and experimental results show that feedforward neural networks with the Bayesian regularization learning algorithm provide the best flow rate estimates. Finally, the benefits of using this soft sensing technique combined with Venturi constriction in open channels are discussed. PMID:29072595

Capillary pumping independent of the liquid surface energy and viscosity

NASA Astrophysics Data System (ADS)

Guo, Weijin; Hansson, Jonas; van der Wijngaart, Wouter

2018-03-01

Capillary pumping is an attractive means of liquid actuation because it is a passive mechanism, i.e., it does not rely on an external energy supply during operation. The capillary flow rate generally depends on the liquid sample viscosity and surface energy. This poses a problem for capillary-driven systems that rely on a predictable flow rate and for which the sample viscosity or surface energy are not precisely known. Here, we introduce the capillary pumping of sample liquids with a flow rate that is constant in time and independent of the sample viscosity and sample surface energy. These features are enabled by a design in which a well-characterized pump liquid is capillarily imbibed into the downstream section of the pump and thereby pulls the unknown sample liquid into the upstream pump section. The downstream pump geometry is designed to exert a Laplace pressure and fluidic resistance that are substantially larger than those exerted by the upstream pump geometry on the sample liquid. Hence, the influence of the unknown sample liquid on the flow rate is negligible. We experimentally tested pumps of the new design with a variety of sample liquids, including water, different samples of whole blood, different samples of urine, isopropanol, mineral oil, and glycerol. The capillary filling speeds of these liquids vary by more than a factor 1000 when imbibed to a standard constant cross-section glass capillary. In our new pump design, 20 filling tests involving these liquid samples with vastly different properties resulted in a constant volumetric flow rate in the range of 20.96-24.76 μL/min. We expect this novel capillary design to have immediate applications in lab-on-a-chip systems and diagnostic devices.

Determining a membrane's shear modulus, independent of its area-dilatation modulus, via capsule flow in a converging micro-capillary.

PubMed

Dimitrakopoulos, P; Kuriakose, S

2015-04-14

Determination of the elastic properties of the membrane of artificial capsules is essential for the better design of the various devices that are utilized in their engineering and biomedical applications. However this task is complicated owing to the combined effects of the shear and area-dilatation moduli on the capsule deformation. Based on computational investigation, we propose a new methodology to determine a membrane's shear modulus, independent of its area-dilatation modulus, by flowing strain-hardening capsules in a converging micro-capillary of comparable size under Stokes flow conditions, and comparing the experimental measurements of the capsule elongation overshooting with computational data. The capsule prestress, if any, can also be determined with the same methodology. The elongation overshooting is practically independent of the viscosity ratio for low and moderate viscosity ratios, and thus a wide range of capsule fluids can be employed. Our proposed experimental device can be readily produced via glass fabrication while owing to the continuous flow in the micro-capillary, the characterization of a large number of artificial capsules is possible.

The effect of a microscale fracture on dynamic capillary pressure of two-phase flow in porous media

NASA Astrophysics Data System (ADS)

Tang, Mingming; Lu, Shuangfang; Zhan, Hongbin; Wenqjie, Guo; Ma, Huifang

2018-03-01

Dynamic capillary pressure (DCP) effects, which is vital for predicting multiphase flow behavior in porous media, refers to the injection rate dependence capillary pressure observed during non-equilibrium displacement experiments. However, a clear picture of the effects of microscale fractures on DCP remains elusive. This study quantified the effects of microscale fractures on DCP and simulated pore-scale force and saturation change in fractured porous media using the multiphase lattice Boltzmann method (LBM). Eighteen simulation cases were carried out to calculate DCP as a function of wetting phase saturation. The effects of viscosity ratio and fracture orientation, aperture and length on DCP and DCP coefficient τ were investigated, where τ refers to the ratio of the difference of DCP and static capillary pressure (SCP) over the rate of wetting-phase saturation change versus time. Significant differences in τ values were observed between unfractured and fractured porous media. The τ values of fractured porous media were 1.1  × 104 Pa ms to 5.68 × 105 Pa ms, which were one or two orders of magnitude lower than those of unfractured porous media with a value of 4 × 106 Pa. ms. A horizontal fracture had greater effects on DCP and τ than a vertical fracture, given the same fracture aperture and length. This study suggested that a microscale fracture might result in large magnitude changes in DCP for two-phase flow.

Gas flow in plant microfluidic networks controlled by capillary valves

NASA Astrophysics Data System (ADS)

Capron, M.; Tordjeman, Ph.; Charru, F.; Badel, E.; Cochard, H.

2014-03-01

The xylem vessels of trees constitute a model natural microfluidic system. In this work, we have studied the mechanism of air flow in the Populus xylem. The vessel microstructure was characterized by optical microscopy, transmission electronic microscopy (TEM), and atomic force microscopy (AFM) at different length scales. The xylem vessels have length ≈15 cm and diameter ≈20μm. Flow from one vessel to the next occurs through ˜102 pits, which are grouped together at the ends of the vessels. The pits contain a thin, porous pit membrane with a thickness of 310 nm. We have measured the Young's moduli of the vessel wall and of the pits (both water-saturated and after drying) by specific nanoindentation and nanoflexion experiments with AFM. We found that both the dried and water-saturated pit membranes have Young's modulus around 0.4 MPa, in agreement with values obtained by micromolding of pits deformed by an applied pressure difference. Air injection experiments reveal that air flows through the xylem vessels when the differential pressure across a sample is larger than a critical value ΔPc=1.8 MPa. In order to model the air flow rate for ΔP ⩾ΔPc, we assumed the pit membrane to be a porous medium that is strained by the applied pressure difference. Water menisci in the pit pores play the role of capillary valves, which open at ΔP =ΔPc. From the point of view of the plant physiology, this work presents a basic understanding of the physics of bordered pits.

Experimental consideration of capillary chromatography based on tube radial distribution of ternary mixture carrier solvents under laminar flow conditions.

PubMed

Jinno, Naoya; Hashimoto, Masahiko; Tsukagoshi, Kazuhiko

2011-01-01

A capillary chromatography system has been developed based on the tube radial distribution of the carrier solvents using an open capillary tube and a water-acetonitrile-ethyl acetate mixture carrier solution. This tube radial distribution chromatography (TRDC) system works under laminar flow conditions. In this study, a phase diagram for the ternary mixture carrier solvents of water, acetonitrile, and ethyl acetate was constructed. The phase diagram that included a boundary curve between homogeneous and heterogeneous solutions was considered together with the component ratios of the solvents in the homogeneous carrier solutions required for the TRDC system. It was found that the TRDC system performed well with homogeneous solutions having component ratios of the solvents that were positioned near the homogeneous-heterogeneous solution boundary of the phase diagram. For preparing the carrier solutions of water-hydrophilic/hydrophobic organic solvents for the TRDC system, we used for the first time methanol, ethanol, 1,4-dioxane, and 1-propanol, instead of acetonitrile (hydrophilic organic solvent), as well as chloroform and 1-butanol, instead of ethyl acetate (hydrophobic organic solvent). The homogeneous ternary mixture carrier solutions were prepared near the homogeneous-heterogeneous solution boundary. Analyte mixtures of 2,6-naphthalenedisulfonic acid and 1-naphthol were separated with the TRDC system using these homogeneous ternary mixture carrier solutions. The pressure change in the capillary tube under laminar flow conditions might alter the carrier solution from homogeneous in the batch vessel to heterogeneous, thus affecting the tube radial distribution of the solvents in the capillary tube.

Ultrasonically Encoded Photoacoustic Flowgraphy in Biological Tissue

NASA Astrophysics Data System (ADS)

Wang, Lidai; Xia, Jun; Yao, Junjie; Maslov, Konstantin I.; Wang, Lihong V.

2013-11-01

Blood flow speed is an important functional parameter. Doppler ultrasound flowmetry lacks sufficient sensitivity to slow blood flow (several to tens of millimeters per second) in deep tissue. To address this challenge, we developed ultrasonically encoded photoacoustic flowgraphy combining ultrasonic thermal tagging with photoacoustic imaging. Focused ultrasound generates a confined heat source in acoustically absorptive fluid. Thermal waves propagate with the flow and are directly visualized in pseudo color using photoacoustic computed tomography. The Doppler shift is employed to calculate the flow speed. This method requires only acoustic and optical absorption, and thus is applicable to continuous fluid. A blood flow speed as low as 0.24mm·s-1 was successfully measured. Deep blood flow imaging was experimentally demonstrated under 5-mm-thick chicken breast tissue.

Capillary flow of amorphous metal for high performance electrode

PubMed Central

Kim, Se Yun; Kim, Suk Jun; Jee, Sang Soo; Park, Jin Man; Park, Keum Hwan; Park, Sung Chan; Cho, Eun Ae; Lee, Jun Ho; Song, In Yong; Lee, Sang Mock; Han, In Taek; Lim, Ka Ram; Kim, Won Tae; Park, Ju Cheol; Eckert, Jürgen; Kim, Do Hyang; Lee, Eun-Sung

2013-01-01

Metallic glass (MG) assists electrical contact of screen-printed silver electrodes and leads to comparable electrode performance to that of electroplated electrodes. For high electrode performance, MG needs to be infiltrated into nanometer-scale cavities between Ag particles and reacts with them. Here, we show that the MG in the supercooled state can fill the gap between Ag particles within a remarkably short time due to capillary effect. The flow behavior of the MG is revealed by computational fluid dynamics and density funtional theory simulation. Also, we suggest the formation mechanism of the Ag electrodes, and demonstrate the criteria of MG for higher electrode performance. Consequently, when Al85Ni5Y8Co2 MG is added in the Ag electrodes, cell efficiency is enhanced up to 20.30% which is the highest efficiency reported so far for screen-printed interdigitated back contact solar cells. These results show the possibility for the replacement of electroplating process to screen-printing process. PMID:23851671

Capillary red blood cell velocimetry by phase-resolved optical coherence tomography

NASA Astrophysics Data System (ADS)

Tang, Jianbo; Erdener, Sefik Evren; Fu, Buyin; Boas, David A.

2018-02-01

Quantitative measurement of blood flow velocity in capillaries is challenging due to their small size (around 5-10 μm), and the discontinuity and single-file feature of RBCs flowing in a capillary. In this work, we present a phase-resolved Optical Coherence Tomography (OCT) method for accurate measurement of the red blood cell (RBC) speed in cerebral capillaries. To account for the discontinuity of RBCs flowing in capillaries, we applied an M-mode scanning strategy that repeated A-scans at each scanning position for an extended time. As the capillary size is comparable to the OCT resolution size (3.5×3.5×3.5μm), we applied a high pass filter to remove the stationary signal component so that the phase information of the dynamic component (i.e. from the moving RBC) could be enhanced to provide an accurate estimate of the RBC axial speed. The phase-resolved OCT method accurately quantifies the axial velocity of RBC's from the phase shift of the dynamic component of the signal. We validated our measurements by RBC passage velocimetry using the signal magnitude of the same OCT time series data. These proposed method of capillary velocimetry proved to be a robust method of mapping capillary RBC speeds across the micro-vascular network.

Cerebral blood flow reduction in Alzheimer's disease: impact of capillary occlusions on mice and humans

NASA Astrophysics Data System (ADS)

Berg, Maxime; Merlo, Adlan; Peyrounette, Myriam; Doyeux, Vincent; Smith, Amy; Cruz-Hernandez, Jean; Bracko, Oliver; Haft-Javaherian, Mohammad; Nishimura, Nozomi; Schaffer, Chris B.; Davit, Yohan; Quintard, Michel; Lorthois, Sylvie

2017-11-01

Alzheimer's disease may be the most common form of dementia, yet a satisfactory diagnosis procedure has still to be found. Recent studies suggest that a significant decrease of cerebral blood flow, probably caused by white blood cells stalling small vessels, may be among the earliest biological markers. To assess this hypothesis we derive a blood flow model, validate it against in vitro controlled experiments and in vivo measurements made on mice. We then investigate the influence of capillary occlusions on regional perfusion (sum of all arteriole flowrates feeding the network) of large mice and humans anatomical networks. Consistent with experiments, we observe no threshold effect, so that even a small percentage of occlusions (2-4%) leads to significant blood flow decrease (5-12%). We show that both species share the same linear dependance, suggesting possible translation from mice to human. ERC BrainMicroFlow GA61510, CALMIP HPC (Grant 2017-1541).

Study on internal flow and surface deformation of large droplet levitated by ultrasonic wave.

PubMed

Abe, Yutaka; Hyuga, Daisuke; Yamada, Shogo; Aoki, Kazuyoshi

2006-09-01

It is expected that new materials will be manufactured with containerless processing under the microgravity environment in space. Under the microgravity environment, handling technology of molten metal is important for such processes. There are a lot of previous studies about droplet levitation technologies, including the use of acoustic waves, as the holding technology. However, experimental and analytical information about the relationship between surface deformation and internal flow of a large levitated droplet is still unknown. The purpose of this study is to experimentally investigate the large droplet behavior levitated by the acoustic wave field and its internal flow. To achieve this, first, numerical simulation is conducted to clarify the characteristics of acoustic wave field. Second, the levitation characteristic and the internal flow of the levitated droplet are investigated by the ultrasonic standing wave under normal gravity environment. Finally, the levitation characteristic and internal flow of levitated droplet are observed under microgravity in an aircraft to compare results with the experiment performed under the normal gravity environment.

Excitation condition analysis of guided wave on PFA tubes for ultrasonic flow meter.

PubMed

Li, Xuan; Xiao, Xufeng; Cao, Li

2016-12-01

Impurity accumulation, which decreases the accuracy of flow measurement, is a critical problem when applying Z-shaped or U-shaped ultrasonic flow meters on straight PFA tubes. It can be expected that the guided wave can be used to implement flow measurement on straight PFA tubes. In this paper, the propagation of guided wave is explained by finite element simulations for the flow meter design. Conditions of guided wave generation, including the excitation frequency and the wedge structure, are studied in the simulations. The wedge is designed as a cone which is friendly to be manufactured and installed. The cone angle, the piezoelectric wafer's resonant frequency and the vibration directions are studied in the simulations. The simulations shows that the propagation of guided wave in thin PFA tubes is influenced by the piezoelectric wafers' resonant frequency and the vibration direction when the mode is on the 'water line'. Based on the results of the simulations, an experiment is conducted to verify the principles of excitation conditions, which performs flow measurement on a straight PFA tube well. Copyright © 2016 Elsevier B.V. All rights reserved.

[Recent advances and applications of capillary electrochromatography and pressurized capillary electrochromatography].

PubMed

Wu, Yi; Zhang, Xiaohui; Wei, Juan; Xue, Yunyun; Bahatibieke, Marjan; Wang, Yan; Yan, Chao

2009-09-01

Capillary electrochromatography (CEC), in which electroosmotic flow (EOF) created from the electrical double layer is made to act as a pump to drive the mobile phase in a capillary column packed with micro-particulates or coated with stationary phase. Both neutral and charged species can be resolved by CEC. It has been demonstrated that the efficiency of a separation obtained by electroosmotic propulsion is superior to that obtained by pressure-driven flow (as is the case in HPLC). CEC combines the best features of CE and versatile selectivity and large sample capacity of HPLC, promising high efficiency, high resolution, high selectivity and high peak capacity. However, in practice, when CEC is used without pressure, often used on a commercial CE instrument, there are problems and difficulties associated with bubbles formation and column dry-out. These difficulties can be overcome by a pressurized CEC (pCEC) system, in which a supplementary pressure is applied to the column in addition to the EOF. In such a system, a pressure can be applied to the capillary column to suppress bubbles formation. Quantitative sample introduction in pCEC can be easily achieved through a rotary-type injector. Most importantly, it is amenable for a solvent gradient mode, similar to that in HPLC, by programming the composition of mobile phase. The article brings a comprehensive survey of recent development of CEC and pCEC, including the development of instrumentation, capillary columns and stationary phase as well as CEC and pCEC applications in life science, biotechnology, pharmaceutical analysis, food safety and environmental security. Prospects for CEC and pCEC development and application are also discussed.

Multiple breath washout with a sidestream ultrasonic flow sensor and mass spectrometry: a comparative study.

PubMed

Fuchs, Susanne I; Buess, Christian; Lum, Sooky; Kozlowska, Wanda; Stocks, Janet; Gappa, Monika

2006-12-01

Over recent years, there has been renewed interest in the multiple breath wash-out (MBW) technique for assessing ventilation inhomogeneity (VI) as a measure of early lung disease in children. While currently considered the gold standard, use of mass spectrometry (MS) to measure MBW is not commercially available, thereby limiting widespread application of this technique. A mainstream ultrasonic flow sensor was marketed for MBW a few years ago, but its use was limited to infants. We have recently undertaken intensive modifications of both hardware and software for the ultrasonic system to extend its use for older children. The aim of the current in vivo study was to compare simultaneous measurements of end-tidal tracer gas concentrations and lung clearance index (LCI) from this modified ultrasonic device with those from a mass spectrometer. Paired measurements of three MBW, using 4% sulfur hexafluoride (SF(6)) as the tracer gas and the two systems in series, were obtained in nine healthy adult volunteers. End-tidal tracer gas concentrations (n = 675 paired values) demonstrated close agreement (95% CI of difference -0.23; -0.17%, r(2) = 1). FRC was slightly higher from the MS (95%CI 0.08;0.17 L), but there was no difference in LCI (95%CI -0.10; 0.3). We conclude, that this ultrasonic prototype system measures end-tidal tracer gas concentration accurately and may therefore be a valid tool for MBW beyond early childhood. This prototype system could be the basis for a commercial device allowing more widespread application of MBW in the near future.

Ultrasonic monitoring of spontaneous imbibition experiments: Precursory moisture diffusion effects ahead of water front

NASA Astrophysics Data System (ADS)

David, Christian; Sarout, Joël.; Dautriat, Jérémie; Pimienta, Lucas; Michée, Marie; Desrues, Mathilde; Barnes, Christophe

2017-07-01

Fluid substitution processes have been investigated in the laboratory on 14 carbonate and siliciclastic reservoir rock analogues through spontaneous imbibition experiments on vertical cylindrical specimens with simultaneous ultrasonic monitoring and imaging. The motivation of our study was to identify the seismic attributes of fluid substitution in reservoir rocks and to link them to physical processes. It is shown that (i) the P wave velocity either decreases or increases when the capillary front reaches the Fresnel clearance zone, (ii) the P wave amplitude is systematically impacted earlier than the velocity is, (iii) this precursory amplitude decrease occurs when the imbibition front is located outside of the Fresnel zone, and (iv) the relative variation of the P wave amplitude is always much larger than that of the P wave velocity. These results suggest that moisture diffuses into the pore space ahead of the water front. This postulate is further supported by a quantitative analysis of the time evolution of the observed P wave amplitudes. In a sense, P wave amplitude acts as a precursor of the arrival of the capillary front. This phenomenon is used to estimate the effective diffusivity of moisture in the tested rocks. The effective moisture diffusivity estimated from the ultrasonic data is strongly correlated with permeability: a power law with exponent 0.96 predicts permeability from ultrasonic monitoring within a factor 3 without noticeable bias. When the effective diffusivity is high, moisture diffusion affects ultrasonic P wave attributes even before the imbibition starts and impacts the P wave reflectivity as evidenced by the variations recorded in the waveform coda.

Dynamical clustering of red blood cells in capillary vessels.

PubMed

Boryczko, Krzysztof; Dzwinel, Witold; Yuen, David A

2003-02-01

We have modeled the dynamics of a 3-D system consisting of red blood cells (RBCs), plasma and capillary walls using a discrete-particle approach. The blood cells and capillary walls are composed of a mesh of particles interacting with harmonic forces between nearest neighbors. We employ classical mechanics to mimic the elastic properties of RBCs with a biconcave disk composed of a mesh of spring-like particles. The fluid particle method allows for modeling the plasma as a particle ensemble, where each particle represents a collective unit of fluid, which is defined by its mass, moment of inertia, translational and angular momenta. Realistic behavior of blood cells is modeled by considering RBCs and plasma flowing through capillaries of various shapes. Three types of vessels are employed: a pipe with a choking point, a curved vessel and bifurcating capillaries. There is a strong tendency to produce RBC clusters in capillaries. The choking points and other irregularities in geometry influence both the flow and RBC shapes, considerably increasing the clotting effect. We also discuss other clotting factors coming from the physical properties of blood, such as the viscosity of the plasma and the elasticity of the RBCs. Modeling has been carried out with adequate resolution by using 1 to 10 million particles. Discrete particle simulations open a new pathway for modeling the dynamics of complex, viscoelastic fluids at the microscale, where both liquid and solid phases are treated with discrete particles. Figure A snapshot from fluid particle simulation of RBCs flowing along a curved capillary. The red color corresponds to the highest velocity. We can observe aggregation of RBCs at places with the most stagnant plasma flow.

Numerical 3D flow simulation of attached cavitation structures at ultrasonic horn tips and statistical evaluation of flow aggressiveness via load collectives

NASA Astrophysics Data System (ADS)

Mottyll, S.; Skoda, R.

2015-12-01

A compressible inviscid flow solver with barotropic cavitation model is applied to two different ultrasonic horn set-ups and compared to hydrophone, shadowgraphy as well as erosion test data. The statistical analysis of single collapse events in wall-adjacent flow regions allows the determination of the flow aggressiveness via load collectives (cumulative event rate vs collapse pressure), which show an exponential decrease in agreement to studies on hydrodynamic cavitation [1]. A post-processing projection of event rate and collapse pressure on a reference grid reduces the grid dependency significantly. In order to evaluate the erosion-sensitive areas a statistical analysis of transient wall loads is utilised. Predicted erosion sensitive areas as well as temporal pressure and vapour volume evolution are in good agreement to the experimental data.

Immiscible displacement of oil by water in consolidated porous media due to capillary imbibition under ultrasonic waves.

PubMed

Hamida, Tarek; Babadagli, Tayfun

2007-09-01

Numerous studies done in the last four decades have demonstrated that acoustic stimulation may enhance recovery in oil reservoirs. This technology is not only technically feasible, but also serves as an economical, environmentally friendly alternative to currently accepted enhanced oil recovery (EOR) method. It requires low capital expenditure, and yields almost immediate improvement without any additional EOR agents. Despite a vast body of empirical and theoretical support, this method lacks sufficient understanding to make meaningful and consistent engineering predictions. This is in part due to the complex nature of the physical processes involved, as well as due to a shortage of fundamental/experimental research. Much of what the authors believe is happening within acoustically stimulated porous media is speculative and theoretical. This paper focuses on the effects of ultrasound on the interfacial forces between immiscible fluids. Capillary (spontaneous) imbibition of an aqueous phase into oil (or air)-saturated Berea sandstone and Indiana limestone samples experiments were conducted. Solutions of water, brine (15,000 and 150,000 ppm NaCl), anionic surfactant (sodium dodecyl diphenyloxide disulfonate), nonionic surfactant (alcohol ethoxylate) and polymer (xanthan gum) were prepared as the aqueous phase. Both counter-current and co-current geometries were tested. Due to the intrinsically unforced, gentle nature of the process, and their strong dependence on wettability, interfacial tension, viscosity and density, such experiments provide valuable insight into some of the governing mechanisms behind ultrasonic stimulation.

Comparison of the Internal Energy Deposition of Venturi-Assisted Electrospray Ionization and a Venturi-Assisted Array of Micromachined UltraSonic Electrosprays (AMUSE)

PubMed Central

Hampton, Christina Y.; Silvestri, Catherine J.; Forbes, Thomas P.; Varady, Mark J.; Meacham, J. Mark; Fedorov, Andrei G.; Degertekin, F. Levent; Fernández, Facundo M.

2008-01-01

The internal energy deposition of a Venturi-assisted array of micromachined ultrasonic electrosprays (AMUSE), with and without the application of a DC charging potential, is compared with equivalent experiments for Venturi-assisted electrospray ionization (ESI) using the “survival yield” method on a series of para-substituted benzylpyridinium salts. Under conditions previously shown to provide maximum ion yields for standard compounds, the observed mean internal energies were nearly identical (1.93–2.01eV). Operation of AMUSE without nitrogen flow to sustain the air amplifier focusing effect generated energetically-colder ions with mean internal energies that were up to 39% lower than those for ESI. A balance between improved ion transfer, adequate desolvation and favorable ion energetics was achieved by selection of optimum operational ranges for the parameters that most strongly influence the ion population, namely the air amplifier gas flow rate and API capillary temperature. Examination of the energy landscapes obtained for combinations of these parameters showed that a low internal energy region (≤ 1.0 eV) was present at nitrogen flow rates between 2 – 4 L min−1 and capillary temperatures up to 250°C using ESI (9% of all parameter combinations tested). Using AMUSE, this region was present at nitrogen flow rates up to 2.5 L min−1 and all capillary temperatures (13% of combinations tested). The signal-to-noise ratio (S/N) of the intact p-methylbenzylpyridinium ion obtained from a 5 μM mixture of thermometer compounds using AMUSE at the extremes of the studied temperature range was at least 5 times higher than that of ESI demonstrating the potential of AMUSE ionization as a soft method for the characterization of labile species by mass spectrometry. PMID:18650100

Numerical investigations of two-phase flow with dynamic capillary pressure in porous media via a moving mesh method

NASA Astrophysics Data System (ADS)

Zhang, Hong; Zegeling, Paul Andries

2017-09-01

Motivated by observations of saturation overshoot, this paper investigates numerical modeling of two-phase flow in porous media incorporating dynamic capillary pressure. The effects of the dynamic capillary coefficient, the infiltrating flux rate and the initial and boundary values are systematically studied using a traveling wave ansatz and efficient numerical methods. The traveling wave solutions may exhibit monotonic, non-monotonic or plateau-shaped behavior. Special attention is paid to the non-monotonic profiles. The traveling wave results are confirmed by numerically solving the partial differential equation using an accurate adaptive moving mesh solver. Comparisons between the computed solutions using the Brooks-Corey model and the laboratory measurements of saturation overshoot verify the effectiveness of our approach.

Single-Molecule Detection in Micron-Sized Capillaries

NASA Astrophysics Data System (ADS)

Ball, David A.; Shen, Guoqing; Davis, Lloyd M.

2004-11-01

The detection of individual molecules in solution by laser-induced fluorescence is becoming an increasingly important tool for biophysics research and biotechnology applications. In a typical single-molecule detection (SMD) experiment, diffusion is the dominant mode of transport of fluorophores through the focused laser beam. In order to more rapidly process a large number of slowly diffusing bio-molecules for applications in pharmaceutical drug discovery, a flow can be introduced within a capillary. If the flow speed is sufficient, bio-molecules will be carried through the probe volume significantly faster than by diffusion alone. Here we discuss SMD near the tip of, and in, such micron-sized capillaries, with a high numerical-aperture microscope objective used for confocal-epi-illumination along the axis of the capillary. Problems such as molecular adsorption to the glass are also addressed.

Cerebral small vessel disease: Capillary pathways to stroke and cognitive decline

PubMed Central

Engedal, Thorbjørn S; Moreton, Fiona; Hansen, Mikkel B; Wardlaw, Joanna M; Dalkara, Turgay; Markus, Hugh S; Muir, Keith W

2015-01-01

Cerebral small vessel disease (SVD) gives rise to one in five strokes worldwide and constitutes a major source of cognitive decline in the elderly. SVD is known to occur in relation to hypertension, diabetes, smoking, radiation therapy and in a range of inherited and genetic disorders, autoimmune disorders, connective tissue disorders, and infections. Until recently, changes in capillary patency and blood viscosity have received little attention in the aetiopathogenesis of SVD and the high risk of subsequent stroke and cognitive decline. Capillary flow patterns were, however, recently shown to limit the extraction efficacy of oxygen in tissue and capillary dysfunction therefore proposed as a source of stroke-like symptoms and neurodegeneration, even in the absence of physical flow-limiting vascular pathology. In this review, we examine whether capillary flow disturbances may be a shared feature of conditions that represent risk factors for SVD. We then discuss aspects of capillary dysfunction that could be prevented or alleviated and therefore might be of general benefit to patients at risk of SVD, stroke or cognitive decline. PMID:26661176

Ultrasonically-assisted Polymer Molding: An Evaluation

NASA Astrophysics Data System (ADS)

Moles, Matthew; Roy, Anish; Silberschmidt, Vadim

Energy reduction in extrusion and injection molding processes can be achieved by the introduction of ultrasonic energy. Polymer flow can be enhanced on application of ultrasonic vibration, which can reduce the thermal and pressure input requirements to produce the same molding; higher productivity may also be achieved. In this paper, a design of an ultrasound-assisted injection mold machine is explored. An extrusion-die design was augmented with a commercial 1.5 kW ultrasonic transducer and sonotrode designed to resonate close to 20 kHz with up to 100 μm vibration amplitude. The design was evaluated with modal and thermal analysis using finite-element analysis software. The use of numerical techniques, including computational fluid dynamics, fluid-structure interaction and coupled Lagrangian-Eulerian method, to predict the effect of ultrasound on polymer flow was considered. A sonotrode design utilizing ceramic to enhance thermal isolation was also explored.

Investigation of pressure drop in capillary tube for mixed refrigerant Joule-Thomson cryocooler

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ardhapurkar, P. M.; Sridharan, Arunkumar; Atrey, M. D.

2014-01-29

A capillary tube is commonly used in small capacity refrigeration and air-conditioning systems. It is also a preferred expansion device in mixed refrigerant Joule-Thomson (MR J-T) cryocoolers, since it is inexpensive and simple in configuration. However, the flow inside a capillary tube is complex, since flashing process that occurs in case of refrigeration and air-conditioning systems is metastable. A mixture of refrigerants such as nitrogen, methane, ethane, propane and iso-butane expands below its inversion temperature in the capillary tube of MR J-T cryocooler and reaches cryogenic temperature. The mass flow rate of refrigerant mixture circulating through capillary tube depends onmore » the pressure difference across it. There are many empirical correlations which predict pressure drop across the capillary tube. However, they have not been tested for refrigerant mixtures and for operating conditions of the cryocooler. The present paper assesses the existing empirical correlations for predicting overall pressure drop across the capillary tube for the MR J-T cryocooler. The empirical correlations refer to homogeneous as well as separated flow models. Experiments are carried out to measure the overall pressure drop across the capillary tube for the cooler. Three different compositions of refrigerant mixture are used to study the pressure drop variations. The predicted overall pressure drop across the capillary tube is compared with the experimentally obtained value. The predictions obtained using homogeneous model show better match with the experimental results compared to separated flow models.« less

Improvements in Low-cost Ultrasonic Measurements of Blood Flow in "by-passes" Using Narrow & Broad Band Transit-time Procedures

NASA Astrophysics Data System (ADS)

Ramos, A.; Calas, H.; Diez, L.; Moreno, E.; Prohías, J.; Villar, A.; Carrillo, E.; Jiménez, A.; Pereira, W. C. A.; Von Krüger, M. A.

The cardio-pathology by ischemia is an important cause of death, but the re-vascularization of coronary arteries (by-pass operation) is an useful solution to reduce associated morbidity improving quality of life in patients. During these surgeries, the flow in coronary vessels must be measured, using non-invasive ultrasonic methods, known as transit time flow measurements (TTFM), which are the most accurate option nowadays. TTFM is a common intra-operative tool, in conjunction with classic Doppler velocimetry, to check the quality of these surgery processes for implanting grafts in parallel with the coronary arteries. This work shows important improvements achieved in flow-metering, obtained in our research laboratories (CSIC, ICIMAF, COPPE) and tested under real surgical conditions in Cardiocentro-HHA, for both narrowband NB and broadband BB regimes, by applying results of a CYTED multinational project (Ultrasonic & computational systems for cardiovascular diagnostics). mathematical models and phantoms were created to evaluate accurately flow measurements, in laboratory conditions, before our new electronic designs and low-cost implementations, improving previous ttfm systems, which include analogic detection, acquisition & post-processing, and a portable PC. Both regimes (NB and BB), with complementary performances for different conditions, were considered. Finally, specific software was developed to offer facilities to surgeons in their interventions.

High temporal resolution coupling of low-flow push-pull perfusion to capillary electrophoresis for ascorbate analysis at the rat vitreoretinal interface.

PubMed

Patterson, Eric E; Pritchett, Jeanita S; Shippy, Scott A

2009-02-01

A system is presented demonstrating the high-temporal resolution coupling of low-flow push-pull perfusion sampling (LFPS) to capillary electrophoresis for the absorbance measurement of ascorbate at the rat vitreoretinal interface. This system holds all separation components at a low pressure as the means for withdrawing sample during LFPS. The system uses a flow-gated interface to directly couple the withdrawal capillary from the LFPS probe to a separation capillary and eliminates the need for any offline sample handling. The temporal resolution of the system was limited by injection time and is less than 16 s. This high temporal resolution was applied to the monitoring of in vivo ascorbate levels at the rat vitreoretinal interface. Baseline concentrations of ascorbate were found to be 86 microM +/- 18 microM at the vitreoretinal interface. Baseline concentrations matched well with those obtained for the postmortem bulk vitreous analysis. Upon stimulation with 145 mM K(+), a maximum increase in baseline values between 32-107% for n = 3 was observed. This system demonstrates the first in vivo temporal study of ascorbate at the rat vitreoretinal interface.

The effect of image alignment on capillary blood flow measurement of the neuroretinal rim using the Heidelberg retina flowmeter

PubMed Central

Sehi, M; Flanagan, J G

2004-01-01

Aim: To examine the influence of image alignment on the repeatability of blood flow measurements of the optic nerve. Methods: 10 normal subjects were examined. Heidelberg retina tomograph imaging was performed to establish best location and focus for the temporal neuroretinal rim. Two high quality Heidelberg retina flowmeter (HRF) images were acquired for three methods of alignment: central, nasal, and temporal. A 10×10 pixel measurement window was selected and exactly reproduced on all images. The interquartile pixel values were used to calculate capillary flow. ANOVA, intraclass correlation coefficients (ICC) and the coefficient of repeatability (CoR) were used for analysis. Results: There was no difference between methods (p = 0.47) or between visits (p = 0.51). The ICCs were 0.83 for the central, 0.34 for the nasal, and 0.42 for the temporal alignment. The CoR was 31.5 for central (mean effect 235.1), 234.6 for nasal, and 256.7 for temporal alignment. Conclusion: Central alignment was the most repeatable method for the measurement of neuroretinal rim capillary blood flow using the HRF. PMID:14736775

Microgravity Investigation of Capillary Driven Imbibition

NASA Astrophysics Data System (ADS)

Dushin, V. R.; Nikitin, V. F.; Smirnov, N. N.; Skryleva, E. I.; Tyurenkova, V. V.

2018-05-01

The goal of the present paper is to investigate the capillary driven filtration in porous media under microgravity conditions. New mathematical model that allows taking into account the blurring of the front due to the instability of the displacement that is developing at the front is proposed. The constants in the mathematical model were selected on the basis of the experimental data on imbibition into unsaturated porous media under microgravity conditions. The flow under the action of a combination of capillary forces and a constant pressure drop or a constant flux is considered. The effect of capillary forces and the type of wettability of the medium on the displacement process is studied. A criterion in which case the capillary effects are insignificant and can be neglected is established.

Recent progress in online ultrasonic process monitoring

NASA Astrophysics Data System (ADS)

Wen, Szu-Sheng L.; Chen, Tzu-Fang; Ramos-Franca, Demartonne; Nguyen, Ky T.; Jen, Cheng-Kuei; Ihara, Ikuo; Derdouri, A.; Garcia-Rejon, Andres

1998-03-01

On-line ultrasonic monitoring of polymer co-extrusion and gas-assisted injection molding are presented. During the co- extrusion of high density polyethylene and Santoprene ultrasonic sensors consisting of piezoelectric transducers and clad ultrasonic buffer rods are used to detect the interface between these two polymers and the stability of the extrusion. The same ultrasonic sensor also measures the surface temperature of the extruded polymer. The results indicate that temperature measurements using ultrasound have a faster response time than those obtained by conventional thermocouple. In gas-assisted injection molding the polymer and gas flow front positions are monitored simultaneously. This information may be used to control the plunger movement.

Method of making tapered capillary tips with constant inner diameters

DOEpatents

Kelly, Ryan T [West Richland, WA; Page, Jason S [Kennewick, WA; Tang, Keqi [Richland, WA; Smith, Richard D [Richland, WA

2009-02-17

Methods of forming electrospray ionization emitter tips are disclosed herein. In one embodiment, an end portion of a capillary tube can be immersed into an etchant, wherein the etchant forms a concave meniscus on the outer surface of the capillary. Variable etching rates in the meniscus can cause an external taper to form. While etching the outer surface of the capillary wall, a fluid can be flowed through the interior of the capillary tube. Etching continues until the immersed portion of the capillary tube is completely etched away.

Rational design of capillary-driven flows for paper-based microfluidics.

PubMed

Elizalde, Emanuel; Urteaga, Raúl; Berli, Claudio L A

2015-05-21

The design of paper-based assays that integrate passive pumping requires a precise programming of the fluid transport, which has to be encoded in the geometrical shape of the substrate. This requirement becomes critical in multiple-step processes, where fluid handling must be accurate and reproducible for each operation. The present work theoretically investigates the capillary imbibition in paper-like substrates to better understand fluid transport in terms of the macroscopic geometry of the flow domain. A fluid dynamic model was derived for homogeneous porous substrates with arbitrary cross-sectional shapes, which allows one to determine the cross-sectional profile required for a prescribed fluid velocity or mass transport rate. An extension of the model to slit microchannels is also demonstrated. Calculations were validated by experiments with prototypes fabricated in our lab. The proposed method constitutes a valuable tool for the rational design of paper-based assays.

Fast separation of enantiomers by capillary electrophoresis using a combination of two capillaries with different internal diameters.

PubMed

Šebestová, Andrea; Petr, Jan

2017-12-01

The combination of capillaries with different internal diameters was used to accelerate the separation of enantiomers in capillary electrophoresis. Separation of R,S-1,1'-binaphthalene-2,2'-diyl hydrogen phosphate using isopropyl derivative of cyclofructan 6 was studied as a model system. The best separation conditions included 500 mM sodium borate pH 9.5 with 60 mM concentration of the chiral selector. Separation lasted approx. 1.5 min using the combination of 50 and 100 μm id capillaries of 9.7 cm and 22.9 cm, respectively. It allowed approx. 12-fold acceleration in comparison to the traditional long-end separation mainly due to the higher electroosmotic flow generated in the connected capillaries. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Capillary flow-driven microfluidic device with wettability gradient and sedimentation effects for blood plasma separation.

PubMed

Maria, M Sneha; Rakesh, P E; Chandra, T S; Sen, A K

2017-03-03

We report a capillary flow-driven microfluidic device for blood-plasma separation that comprises a cylindrical well between a pair of bottom and top channels. Exposure of the well to oxygen-plasma creates wettability gradient on its inner surface with its ends hydrophilic and middle portion hydrophobic. Due to capillary action, sample blood self-infuses into bottom channel and rises up the well. Separation of plasma occurs at the hydrophobic patch due to formation of a 'self-built-in filter' and sedimentation. Capillary velocity is predicted using a model and validated using experimental data. Sedimentation of RBCs is explained using modified Steinour's model and correlation between settling velocity and liquid concentration is found. Variation of contact angle on inner surface of the well is characterized and effects of well diameter and height and dilution ratio on plasma separation rate are investigated. With a well of 1.0 mm diameter and 4.0 mm height, 2.0 μl of plasma was obtained (from <10 μl whole blood) in 15 min with a purification efficiency of 99.9%. Detection of glucose was demonstrated with the plasma obtained. Wetting property of channels was maintained by storing in DI water under vacuum and performance of the device was found to be unaffected over three weeks.

Capillary flow-driven microfluidic device with wettability gradient and sedimentation effects for blood plasma separation

PubMed Central

Maria, M. Sneha; Rakesh, P. E.; Chandra, T. S.; Sen, A. K.

2017-01-01

We report a capillary flow-driven microfluidic device for blood-plasma separation that comprises a cylindrical well between a pair of bottom and top channels. Exposure of the well to oxygen-plasma creates wettability gradient on its inner surface with its ends hydrophilic and middle portion hydrophobic. Due to capillary action, sample blood self-infuses into bottom channel and rises up the well. Separation of plasma occurs at the hydrophobic patch due to formation of a ‘self-built-in filter’ and sedimentation. Capillary velocity is predicted using a model and validated using experimental data. Sedimentation of RBCs is explained using modified Steinour’s model and correlation between settling velocity and liquid concentration is found. Variation of contact angle on inner surface of the well is characterized and effects of well diameter and height and dilution ratio on plasma separation rate are investigated. With a well of 1.0 mm diameter and 4.0 mm height, 2.0 μl of plasma was obtained (from <10 μl whole blood) in 15 min with a purification efficiency of 99.9%. Detection of glucose was demonstrated with the plasma obtained. Wetting property of channels was maintained by storing in DI water under vacuum and performance of the device was found to be unaffected over three weeks. PMID:28256564

Interfacial Dynamics of Condensing Vapor Bubbles in an Ultrasonic Acoustic Field

NASA Astrophysics Data System (ADS)

Boziuk, Thomas; Smith, Marc; Glezer, Ari

2016-11-01

Enhancement of vapor condensation in quiescent subcooled liquid using ultrasonic actuation is investigated experimentally. The vapor bubbles are formed by direct injection from a pressurized steam reservoir through nozzles of varying characteristic diameters, and are advected within an acoustic field of programmable intensity. While kHz-range acoustic actuation typically couples to capillary instability of the vapor-liquid interface, ultrasonic (MHz-range) actuation leads to the formation of a liquid spout that penetrates into the vapor bubble and significantly increases its surface area and therefore condensation rate. Focusing of the ultrasonic beam along the spout leads to ejection of small-scale droplets from that are propelled towards the vapor liquid interface and result in localized acceleration of the condensation. High-speed video of Schlieren images is used to investigate the effects of the ultrasonic actuation on the thermal boundary layer on the liquid side of the vapor-liquid interface and its effect on the condensation rate, and the liquid motion during condensation is investigated using high-magnification PIV measurements. High-speed image processing is used to assess the effect of the actuation on the dynamics and temporal variation in characteristic scale (and condensation rate) of the vapor bubbles.

Direct numerical simulation of gas-solid-liquid flows with capillary effects: An application to liquid bridge forces between spherical particles.

PubMed

Sun, Xiaosong; Sakai, Mikio

2016-12-01

In this study, a numerical method is developed to perform the direct numerical simulation (DNS) of gas-solid-liquid flows involving capillary effects. The volume-of-fluid method employed to track the free surface and the immersed boundary method is adopted for the fluid-particle coupling in three-phase flows. This numerical method is able to fully resolve the hydrodynamic force and capillary force as well as the particle motions arising from complicated gas-solid-liquid interactions. We present its application to liquid bridges among spherical particles in this paper. By using the DNS method, we obtain the static bridge force as a function of the liquid volume, contact angle, and separation distance. The results from the DNS are compared with theoretical equations and other solutions to examine its validity and suitability for modeling capillary bridges. Particularly, the nontrivial liquid bridges formed in triangular and tetrahedral particle clusters are calculated and some preliminary results are reported. We also perform dynamic simulations of liquid bridge ruptures subject to axial stretching and particle motions driven by liquid bridge action, for which accurate predictions are obtained with respect to the critical rupture distance and the equilibrium particle position, respectively. As shown through the simulations, the strength of the present method is the ability to predict the liquid bridge problem under general conditions, from which models of liquid bridge actions may be constructed without limitations. Therefore, it is believed that this DNS method can be a useful tool to improve the understanding and modeling of liquid bridges formed in complex gas-solid-liquid flows.

Nonlinear Dynamics of Formation of Drops of Non-Newtonian Liquids from Capillaries: Satellite Formation and Flow Transitions

NASA Astrophysics Data System (ADS)

Yildirim, Ozgur E.; Basaran, Osman A.

1999-11-01

Drop formation from capillaries, and the often undesired phenomenon of satellite generation, play a central role in diverse applications including ink-jet printing, biochip processors, and spray coating, where the working fluid is usually non-Newtonian. Although some work has been done in related areas, the phenomenon of formation of drops of non--Newtonian fluids from capillaries has remained largely unexplored. Here a theoretical approach is adopted to study the dripping of axisymmetric drops of non--Newtonian liquids from capillaries. The constitutive equation used accounts for both shear thinning and strain hardening. First, regular perturbation theory is utilized to reduce the spatial dimension of the governing equations to one. The computations rely on Galerkin/finite element analysis with adaptive finite differencing for time integration. The dynamics are followed beyond the first breakup to investigate conditions for occurrence of satellites. Effect of increasing flow rate is also studied to uncover transitions that occur as one moves from a regime of periodic drop formation to one of jetting.

Cement-based materials' characterization using ultrasonic attenuation

NASA Astrophysics Data System (ADS)

Punurai, Wonsiri

relationship between attenuation and water to cement (w/c) ratio. A phenomenological model based on the existence of fluid-filled capillary voids is used to help explain the experimentally observed behavior. Overall this research shows the potential of using ultrasonic attenuation to quantitatively characterize cement paste. The absorption and scattering losses can be related to the individual microstructural elements of hardened cement paste. By taking a fundamental, mechanics-based approach, it should be possible to add additional components such as scattering by aggregates or even microcracks in a systematic fashion and eventually build a realistic model for ultrasonic wave propagation study for concrete.

Streptavidin-functionalized capillary immune microreactor for highly efficient chemiluminescent immunoassay.

PubMed

Yang, Zhanjun; Zong, Chen; Ju, Huangxian; Yan, Feng

2011-11-07

A streptavidin functionalized capillary immune microreactor was designed for highly efficient flow-through chemiluminescent (CL) immunoassay. The functionalized capillary could be used as both a support for highly efficient immobilization of antibody and a flow cell for flow-through immunoassay. The functionalized inner wall and the capture process were characterized using scanning electron microscopy. Compared to conventional packed tube or thin-layer cell immunoreactor, the proposed microreactor showed remarkable properties such as lower cost, simpler fabrication, better practicality and wider dynamic range for fast CL immunoassay with good reproducibility and stability. Using α-fetoprotein as model analyte, the highly efficient CL flow-through immunoassay system showed a linear range of 3 orders of magnitude from 0.5 to 200 ng mL(-1) and a low detection limit of 0.1 ng mL(-1). The capillary immune microreactor could make up the shortcoming of conventional CL immunoreactors and provided a promising alternative for highly efficient flow-injection immunoassay. Copyright © 2011 Elsevier B.V. All rights reserved.

Capillary Movement in Substrates in Microgravity

NASA Technical Reports Server (NTRS)

Bula, R. J.; Duffie, N. A.

1996-01-01

A more complete understanding of the dynamics of capillary flow through an unsaturated porous medium would be useful for a number of space and terrestrial applications. Knowledge of capillary migration of liquids in granular beds in microgravity would significantly enhance the development and understanding of how a matrix based nutrient delivery system for the growth of plants would function in a microgravity environment. Thus, such information is of interest from the theoretical as well as practical point of view.

Noninvasive measurement of cerebrospinal fluid flow using an ultrasonic transit time flow sensor: a preliminary study.

PubMed

Pennell, Thomas; Yi, Juneyoung L; Kaufman, Bruce A; Krishnamurthy, Satish

2016-03-01

OBJECT Mechanical failure-which is the primary cause of CSF shunt malfunction-is not readily diagnosed, and the specific reasons for mechanical failure are not easily discerned. Prior attempts to measure CSF flow noninvasively have lacked the ability to either quantitatively or qualitatively obtain data. To address these needs, this preliminary study evaluates an ultrasonic transit time flow sensor in pediatric and adult patients with external ventricular drains (EVDs). One goal was to confirm the stated accuracy of the sensor in a clinical setting. A second goal was to observe the sensor's capability to record real-time continuous CSF flow. The final goal was to observe recordings during instances of flow blockage or lack of flow in order to determine the sensor's ability to identify these changes. METHODS A total of 5 pediatric and 11 adult patients who had received EVDs for the treatment of hydrocephalus were studied in a hospital setting. The primary EVD was connected to a secondary study EVD that contained a fluid-filled pressure transducer and an in-line transit time flow sensor. Comparisons were made between the weight of the drainage bag and the flow measured via the sensor in order to confirm its accuracy. Data from the pressure transducer and the flow sensor were recorded continuously at 100 Hz for a period of 24 hours by a data acquisition system, while the hourly CSF flow into the drip chamber was recorded manually. Changes in the patient's neurological status and their time points were noted. RESULTS The flow sensor demonstrated a proven accuracy of ± 15% or ± 2 ml/hr. The flow sensor allowed real-time continuous flow waveform data recordings. Dynamic analysis of CSF flow waveforms allowed the calculation of the pressure-volume index. Lastly, the sensor was able to diagnose a blocked catheter and distinguish between the blockage and lack of flow. CONCLUSIONS The Transonic flow sensor accurately measures CSF output within ± 15% or ± 2 ml

Surface-directed capillary system; theory, experiments and applications.

PubMed

Bouaidat, Salim; Hansen, Ole; Bruus, Henrik; Berendsen, Christian; Bau-Madsen, Niels Kristian; Thomsen, Peter; Wolff, Anders; Jonsmann, Jacques

2005-08-01

We present a capillary flow system for liquid transport in microsystems. Our simple microfluidic system consists of two planar parallel surfaces, separated by spacers. One of the surfaces is entirely hydrophobic, the other mainly hydrophobic, but with hydrophilic pathways defined on it by photolithographic means. By controlling the wetting properties of the surfaces in this manner, the liquid can be confined to certain areas defined by the hydrophilic pathways. This technique eliminates the need for alignment of the two surfaces. Patterned plasma-polymerized hexafluoropropene constitutes the hydrophobic areas, whereas the untreated glass surface constitutes the hydrophilic pathways. We developed a theoretical model of the capillary flow and obtained analytical solutions which are in good agreement with the experimental results. The capillarity-driven microflow system was also used to pattern and immobilize biological material on planar substrates: well-defined 200 microm wide strips of human cells (HeLa) and fluorescence labelled proteins (fluorescein isothiocyanate-labelled bovine serum albumin, i.e., FITC-BSA) were fabricated using the capillary flow system presented here.

Application of partial inversion pulse to ultrasonic time-domain correlation method to measure the flow rate in a pipe

NASA Astrophysics Data System (ADS)

Wada, Sanehiro; Furuichi, Noriyuki; Shimada, Takashi

2017-11-01

This paper proposes the application of a novel ultrasonic pulse, called a partial inversion pulse (PIP), to the measurement of the velocity profile and flow rate in a pipe using the ultrasound time-domain correlation (UTDC) method. In general, the measured flow rate depends on the velocity profile in the pipe; thus, on-site calibration is the only method of checking the accuracy of on-site flow rate measurements. Flow rate calculation using UTDC is based on the integration of the measured velocity profile. The advantages of this method compared with the ultrasonic pulse Doppler method include the possibility of the velocity range having no limitation and its applicability to flow fields without a sufficient amount of reflectors. However, it has been previously reported that the measurable velocity range for UTDC is limited by false detections. Considering the application of this method to on-site flow fields, the issue of velocity range is important. To reduce the effect of false detections, a PIP signal, which is an ultrasound signal that contains a partially inverted region, was developed in this study. The advantages of the PIP signal are that it requires little additional hardware cost and no additional software cost in comparison with conventional methods. The effects of inversion on the characteristics of the ultrasound transmission were estimated through numerical calculation. Then, experimental measurements were performed at a national standard calibration facility for water flow rate in Japan. The experimental results demonstrate that measurements made using a PIP signal are more accurate and yield a higher detection ratio than measurements using a normal pulse signal.

Multiple capillary biochemical analyzer with barrier member

DOEpatents

Dovichi, Norman J.; Zhang, Jian Z.

1996-01-01

A multiple capillary biochemical analyzer for sequencing DNA and performing other analyses, in which a set of capillaries extends from wells in a microtiter plate into a cuvette. In the cuvette the capillaries are held on fixed closely spaced centers by passing through a sandwich construction having a pair of metal shims which squeeze between them a rubber gasket, forming a leak proof seal for an interior chamber in which the capillary ends are positioned. Sheath fluid enters the chamber and entrains filament sample streams from the capillaries. The filament sample streams, and sheath fluid, flow through aligned holes in a barrier member spaced close to the capillary ends, into a collection chamber having a lower glass window. The filament streams are illuminated above the barrier member by a laser, causing them to fluoresce. The fluorescence is viewed end-on by a CCD camera chip located below the glass window. The arrangement ensures an equal optical path length from all fluorescing spots to the CCD chip and also blocks scattered fluorescence illumination, providing more uniform results and an improved signal to noise ratio.

Study of the Effect of Heat Supply on the Hydrodynamics of the Flow and Heat Transfer in Capillary Elements of Mixing Heads Jet Thrusters

NASA Astrophysics Data System (ADS)

Nigodjuk, V. E.; Sulinov, A. V.

2018-01-01

The article presents the results of experimental studies of hydrodynamics and those of loobman single-phase and two-phase flows in capillary nozzle elements propellant thrusters and the proposed method of their calculation. An experimental study was performed in capillaries with a sharp entrance edge of the internal diameter of 0.16 and 0.33 mm and a relative length 188 and 161, respectively, in pouring distilled water and acetone in the following range of parameters Reynolds number Re = (0,3 ... 10) · 103, Prandtl number Pr = (2 ... 10), pressure p = (0,1 ... 0,3) MPa, the heat flux q = (0...2)×106 W/m2, the difference of temperature under-heating of liquid Δtn = (5 ... 80)K. The dependences for calculation of single phase boundaries, the undeveloped and the developed surface of the bubble and film key singing of subcooled liquid. It is shown theoretically and experimentally confirmed the virtual absence of areas of undeveloped nucleate boiling in laminar flow. The dependence for calculation of hydraulic resistance and heat transfer in the investigated areas of current. It is shown that in the region of nucleate boiling surface in the flow in capillary tubes, influence of the formed vapor phase on the hydrodynamics and heat transfer substantially higher than in larger diameter pipes.

On the performance of capillary barriers as landfill cover

NASA Astrophysics Data System (ADS)

Kämpf, M.; Montenegro, H.

Landfills and waste heaps require an engineered surface cover upon closure. The capping system can vary from a simple soil cover to multiple layers of earth and geosynthetic materials. Conventional design features a compacted soil layer, which suffers from drying out and cracking, as well as root and animal intrusion. Capillary barriers consisting of inclined fine-over-coarse soil layers are investigated as an alternative cover system. Under unsaturated conditions, the textural contrast delays vertical drainage by capillary forces. The moisture that builds up above the contact will flow downdip along the interface of the layers. Theoretical studies of capillary barriers have identified the hydraulic properties of the layers, the inclination angle, the length of the field and the infiltration rate as the fundamental characteristics of the system. However, it is unclear how these findings can lead to design criteria for capillary barriers. To assess the uncertainty involved in such approaches, experiments have been carried out in a 8 m long flume and on large scale test sites (40 m x 15 m). In addition, the ability of a numerical model to represent the relevant flow processes in capillary barriers has been examined.

Investigation of energy dissipation due to contact angle hysteresis in capillary effect

NASA Astrophysics Data System (ADS)

Athukorallage, Bhagya; Iyer, Ram

2016-06-01

Capillary action or Capillarity is the ability of a liquid to flow in narrow spaces without the assistance of, and in opposition to, external forces like gravity. Three effects contribute to capillary action, namely, adhesion of the liquid to the walls of the confining solid; meniscus formation; and low Reynolds number fluid flow. We investigate the dissipation of energy during one cycle of capillary action, when the liquid volume inside a capillary tube first increases and subsequently decreases while assuming quasi-static motion. The quasi-static assumption allows us to focus on the wetting phenomenon of the solid wall by the liquid and the formation of the meniscus. It is well known that the motion of a liquid on an non-ideal surface involves the expenditure of energy due to contact angle hysteresis. In this paper, we derive the equations for the menisci and the flow rules for the change of the contact angles for a liquid column in a capillary tube at a constant temperature and volume by minimizing the Helmholtz free energy using calculus of variations. We describe the numerical solution of these equations and present results from computations for the case of a capillary tube with 1 mm diameter.

Capillary Flow Layer-by-Layer: A Microfluidic Platform for the High-Throughput Assembly and Screening of Nanolayered Film Libraries

PubMed Central

2015-01-01

Layer-by-layer (LbL) assembly is a powerful tool with increasing real world applications in energy, biomaterials, active surfaces, and membranes; however, the current state of the art requires individual sample construction using large quantities of material. Here we describe a technique using capillary flow within a microfluidic device to drive high-throughput assembly of LbL film libraries. This capillary flow layer-by-layer (CF-LbL) method significantly reduces material waste, improves quality control, and expands the potential applications of LbL into new research spaces. The method can be operated as a simple lab benchtop apparatus or combined with liquid-handling robotics to extend the library size. Here we describe and demonstrate the technique and establish its ability to recreate and expand on the known literature for film growth and morphology. We use the same platform to assay biological properties such as cell adhesion and proliferation and ultimately provide an example of the use of this approach to identify LbL films for surface-based DNA transfection of commonly used cell types. PMID:24836460

Trans-skull ultrasonic Doppler system aided by fuzzy logic

NASA Astrophysics Data System (ADS)

Hata, Yutaka; Nakamura, Masato; Yagi, Naomi; Ishikawa, Tomomoto

2012-06-01

This paper describes a trans-skull ultrasonic Doppler system for measuring the blood flow direction in brain under skull. In this system, we use an ultrasonic array probe with the center frequency of 1.0 MHz. The system determines the fuzzy degree of blood flow by Doppler Effect, thereby it locates blood vessel. This Doppler Effect is examined by the center of gravity shift of the frequency magnitudes. In in-vitro experiment, a cow bone was employed as the skull, and three silicon tubes were done as blood vessels, and bubble in water as blood. We received the ultrasonic waves through a protein, the skull and silicon tubes in order. In the system, fuzzy degrees are determined with respect to the Doppler shift, amplitude of the waves and attenuation of the tissues. The fuzzy degrees of bone and blood direction are calculated by them. The experimental results showed that the system successfully visualized the skull and flow direction, compared with the location and flow direction of the phantom. Thus, it detected the flow direction by Doppler Effect under skull, and automatically extracted the region of skull and blood vessel.

Capillary red blood cell velocimetry by phase-resolved optical coherence tomography.

PubMed

Tang, Jianbo; Erdener, Sefik Evren; Fu, Buyin; Boas, David A

2017-10-01

We present a phase-resolved optical coherence tomography (OCT) method to extend Doppler OCT for the accurate measurement of the red blood cell (RBC) velocity in cerebral capillaries. OCT data were acquired with an M-mode scanning strategy (repeated A-scans) to account for the single-file passage of RBCs in a capillary, which were then high-pass filtered to remove the stationary component of the signal to ensure an accurate measurement of phase shift of flowing RBCs. The angular frequency of the signal from flowing RBCs was then quantified from the dynamic component of the signal and used to calculate the axial speed of flowing RBCs in capillaries. We validated our measurement by RBC passage velocimetry using the signal magnitude of the same OCT time series data.

On the linear stability of blood flow through model capillary networks.

PubMed

Davis, Jeffrey M

2014-12-01

Under the approximation that blood behaves as a continuum, a numerical implementation is presented to analyze the linear stability of capillary blood flow through model tree and honeycomb networks that are based on the microvascular structures of biological tissues. The tree network is comprised of a cascade of diverging bifurcations, in which a parent vessel bifurcates into two descendent vessels, while the honeycomb network also contains converging bifurcations, in which two parent vessels merge into one descendent vessel. At diverging bifurcations, a cell partitioning law is required to account for the nonuniform distribution of red blood cells as a function of the flow rate of blood into each descendent vessel. A linearization of the governing equations produces a system of delay differential equations involving the discharge hematocrit entering each network vessel and leads to a nonlinear eigenvalue problem. All eigenvalues in a specified region of the complex plane are captured using a transformation based on contour integrals to construct a linear eigenvalue problem with identical eigenvalues, which are then determined using a standard QR algorithm. The predicted value of the dimensionless exponent in the cell partitioning law at the instability threshold corresponds to a supercritical Hopf bifurcation in numerical simulations of the equations governing unsteady blood flow. Excellent agreement is found between the predictions of the linear stability analysis and nonlinear simulations. The relaxation of the assumption of plug flow made in previous stability analyses typically has a small, quantitative effect on the stability results that depends on the specific network structure. This implementation of the stability analysis can be applied to large networks with arbitrary structure provided only that the connectivity among the network segments is known.

Ultrasonic atomization of liquids in drop-chain acoustic fountains

PubMed Central

Simon, Julianna C.; Sapozhnikov, Oleg A.; Khokhlova, Vera A.; Crum, Lawrence A.; Bailey, Michael R.

2015-01-01

When focused ultrasound waves of moderate intensity in liquid encounter an air interface, a chain of drops emerges from the liquid surface to form what is known as a drop-chain fountain. Atomization, or the emission of micro-droplets, occurs when the acoustic intensity exceeds a liquid-dependent threshold. While the cavitation-wave hypothesis, which states that atomization arises from a combination of capillary-wave instabilities and cavitation bubble oscillations, is currently the most accepted theory of atomization, more data on the roles of cavitation, capillary waves, and even heat deposition or boiling would be valuable. In this paper, we experimentally test whether bubbles are a significant mechanism of atomization in drop-chain fountains. High-speed photography was used to observe the formation and atomization of drop-chain fountains composed of water and other liquids. For a range of ultrasonic frequencies and liquid sound speeds, it was found that the drop diameters approximately equalled the ultrasonic wavelengths. When water was exchanged for other liquids, it was observed that the atomization threshold increased with shear viscosity. Upon heating water, it was found that the time to commence atomization decreased with increasing temperature. Finally, water was atomized in an overpressure chamber where it was found that atomization was significantly diminished when the static pressure was increased. These results indicate that bubbles, generated by either acoustic cavitation or boiling, contribute significantly to atomization in the drop-chain fountain. PMID:25977591

Demonstration of a high repetition rate capillary discharge waveguide

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gonsalves, A. J., E-mail: ajgonsalves@lbl.gov; Pieronek, C.; Daniels, J.

2016-01-21

A hydrogen-filled capillary discharge waveguide operating at kHz repetition rates is presented for parameters relevant to laser plasma acceleration (LPA). The discharge current pulse was optimized for erosion mitigation with laser guiding experiments and MHD simulation. Heat flow simulations and measurements showed modest temperature rise at the capillary wall due to the average heat load at kHz repetition rates with water-cooled capillaries, which is promising for applications of LPAs such as high average power radiation sources.

PARAMETER ESTIMATION OF TWO-FLUID CAPILLARY PRESSURE-SATURATION AND PERMEABILITY FUNCTIONS

EPA Science Inventory

Capillary pressure and permeability functions are crucial to the quantitative description of subsurface flow and transport. Earlier work has demonstrated the feasibility of using the inverse parameter estimation approach in determining these functions if both capillary pressure ...

The effects of capillary transit time heterogeneity (CTH) on brain oxygenation

PubMed Central

Angleys, Hugo; Østergaard, Leif; Jespersen, Sune N

2015-01-01

We recently extended the classic flow–diffusion equation, which relates blood flow to tissue oxygenation, to take capillary transit time heterogeneity (CTH) into account. Realizing that cerebral oxygen availability depends on both cerebral blood flow (CBF) and capillary flow patterns, we have speculated that CTH may be actively regulated and that changes in the capillary morphology and function, as well as in blood rheology, may be involved in the pathogenesis of conditions such as dementia and ischemia-reperfusion injury. The first extended flow–diffusion equation involved simplifying assumptions which may not hold in tissue. Here, we explicitly incorporate the effects of oxygen metabolism on tissue oxygen tension and extraction efficacy, and assess the extent to which the type of capillary transit time distribution affects the overall effects of CTH on flow–metabolism coupling reported earlier. After incorporating tissue oxygen metabolism, our model predicts changes in oxygen consumption and tissue oxygen tension during functional activation in accordance with literature reports. We find that, for large CTH values, a blood flow increase fails to cause significant improvements in oxygen delivery, and can even decrease it; a condition of malignant CTH. These results are found to be largely insensitive to the choice of the transit time distribution. PMID:25669911

Multiple capillary biochemical analyzer with barrier member

DOEpatents

Dovichi, N.J.; Zhang, J.Z.

1996-10-22

A multiple capillary biochemical analyzer is disclosed for sequencing DNA and performing other analyses, in which a set of capillaries extends from wells in a microtiter plate into a cuvette. In the cuvette the capillaries are held on fixed closely spaced centers by passing through a sandwich construction having a pair of metal shims which squeeze between them a rubber gasket, forming a leak proof seal for an interior chamber in which the capillary ends are positioned. Sheath fluid enters the chamber and entrains filament sample streams from the capillaries. The filament sample streams, and sheath fluid, flow through aligned holes in a barrier member spaced close to the capillary ends, into a collection chamber having a lower glass window. The filament streams are illuminated above the barrier member by a laser, causing them to fluoresce. The fluorescence is viewed end-on by a CCD camera chip located below the glass window. The arrangement ensures an equal optical path length from all fluorescing spots to the CCD chip and also blocks scattered fluorescence illumination, providing more uniform results and an improved signal-to-noise ratio. 12 figs.

Visualization of removal of trapped air from the apical region of the straight root canal models generating 2-phase intermittent counter flow during ultrasonically activated irrigation.

PubMed

Peeters, Harry Huiz; Iskandar, Bernard; Suardita, Ketut; Suharto, Djoko

2014-06-01

The purpose of this in vitro study was to obtain a better understanding of the mechanism of irrigant traveling apically and generating 2-phase intermittent counter flow in straight root canal models during activation of the irrigant by ultrasonic means in an endodontic procedure. A high-speed imaging system, with high temporal and spatial resolution (FastCam SA5; Photron, Tokyo, Japan) at a frame rate of 100,000 frames per second using a macro lens (60 mm, f/2.8; Nikon, Tokyo, Japan), was used to visualize, in glass models of root canals, an ultrasonically induced acoustic pressure wave in an EDTA solution environment. A 25-mm stainless steel noncutting file #20 driven by an ultrasonic device (P5 Newtron; Satelec Acteon, Mérignac, France) at power settings of 5 and 7 produced disturbances at the solution-air interface. We found that apically directed travel of the irrigant was caused by disruption of the surface tension at the solution-air interface. This disruption caused by ultrasonic activation energy displaced air in the form of bubbles from the apical region toward the solution. The apical movement of the solution may be attributed to ultrasonically induced wave generation at the solution-air interface, resulting in the removal of trapped air from the root canal and allowing the solution to travel apically in the opposite directions (via a 2-phase intermittent counter flow). Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Impact of capillary rise and recirculation on simulated crop yields

NASA Astrophysics Data System (ADS)

Kroes, Joop; Supit, Iwan; van Dam, Jos; van Walsum, Paul; Mulder, Martin

2018-05-01

Upward soil water flow is a vital supply of water to crops. The purpose of this study is to determine if upward flow and recirculated percolation water can be quantified separately, and to determine the contribution of capillary rise and recirculated water to crop yield and groundwater recharge. Therefore, we performed impact analyses of various soil water flow regimes on grass, maize and potato yields in the Dutch delta. Flow regimes are characterized by soil composition and groundwater depth and derived from a national soil database. The intermittent occurrence of upward flow and its influence on crop growth are simulated with the combined SWAP-WOFOST model using various boundary conditions. Case studies and model experiments are used to illustrate the impact of upward flow on yield and crop growth. This impact is clearly present in situations with relatively shallow groundwater levels (85 % of the Netherlands), where capillary rise is a well-known source of upward flow; but also in free-draining situations the impact of upward flow is considerable. In the latter case recirculated percolation water is the flow source. To make this impact explicit we implemented a synthetic modelling option that stops upward flow from reaching the root zone, without inhibiting percolation. Such a hypothetically moisture-stressed situation compared to a natural one in the presence of shallow groundwater shows mean yield reductions for grassland, maize and potatoes of respectively 26, 3 and 14 % or respectively about 3.7, 0.3 and 1.5 t dry matter per hectare. About half of the withheld water behind these yield effects comes from recirculated percolation water as occurs in free-drainage conditions and the other half comes from increased upward capillary rise. Soil water and crop growth modelling should consider both capillary rise from groundwater and recirculation of percolation water as this improves the accuracy of yield simulations. This also improves the accuracy of the

Auto-positioning ultrasonic transducer system

NASA Technical Reports Server (NTRS)

Buchanan, Randy K. (Inventor)

2010-01-01

An ultrasonic transducer apparatus and process for determining the optimal transducer position for flow measurement along a conduit outer surface. The apparatus includes a transmitting transducer for transmitting an ultrasonic signal, said transducer affixed to a conduit outer surface; a guide rail attached to a receiving transducer for guiding movement of a receiving transducer along the conduit outer surface, wherein the receiving transducer receives an ultrasonic signal from the transmitting transducer and sends a signal to a data acquisition system; and a motor for moving the receiving transducer along the guide rail, wherein the motor is controlled by a controller. The method includes affixing a transmitting transducer to an outer surface of a conduit; moving a receiving transducer on the conduit outer surface, wherein the receiving transducer is moved along a guide rail by a motor; transmitting an ultrasonic signal from the transmitting transducer that is received by the receiving transducer; communicating the signal received by the receiving transducer to a data acquisition and control system; and repeating the moving, transmitting, and communicating along a length of the conduit.

Numerical simulation and experiment on effect of ultrasonic in polymer extrusion processing

NASA Astrophysics Data System (ADS)

Wan, Yue; Fu, ZhiHong; Wei, LingJiao; Zang, Gongzheng; Zhang, Lei

2018-01-01

The influence of ultrasonic wave on the flow field parameters and the precision of extruded products are studied. Firstly, the effect of vibration power on the average velocity of the outlet, the average viscosity of the die section, the average shear rate and the inlet pressure of the die section were studied by using the Polyflow software. Secondly, the effects of ultrasonic strength on the die temperature and the drop of die pressure were studied experimentally by different head temperature and different screw speed. Finally, the relationship between die pressure and extrusion flow rate under different ultrasonic power were studied through experiments.

Ultrasonic Acoustic Emissions from the Sapwood of Cedar and Hemlock 1

PubMed Central

Tyree, Melvin T.; Dixon, Michael A.; Tyree, E. Loeta; Johnson, Robert

1984-01-01

Measurements are reported of ultrasonic acoustic emissions (AEs) measured from sapwood samples of Thuja occidentalis L. and Tsuga canadensis (L.) Carr. during air dehydration. The measurements were undertaken to test the following three hypotheses: (a) Each cavitation event produces one ultrasonic AE. (b) Large tracheids are more likely to cavitate than small tracheids. (c) When stem water potentials are >−0.4 MPa, a significant fraction of the water content of sapwood is held by `capillary forces.' The last two hypotheses were recently discussed at length by M. H. Zimmermann. Experimental evidence consistent with all three hypotheses was obtained. The evidence for each hypothesis respectively is: (a) the cumulative number of AEs nearly equals the number of tracheids in small samples; (b) more water is lost per AE event at the beginning of the dehydration process than at the end, and (c) sapwood samples dehydrated from an initial water potential of 0 MPa lost significantly more water before AEs started than lost by samples dehydrated from an initial water potential of about −0.4 MPa. The extra water held by fully hydrated sapwood samples may have been capillary water as defined by Zimmerman. We also report an improved method for the measurement of the `intensity' of ultrasonic AEs. Intensity is defined here as the area under the positive spikes of the AE signal (plotted as voltage versus time). This method was applied to produce a frequency histogram of the number of AEs versus intensity. A large fraction of the total number of AEs were of low intensity even in small samples (4 mm diameter by 10 mm length). This suggests that the effective `listening distance' for most AEs was less than 5 to 10 mm. PMID:16663774

On-line preconcentration of fluorescent derivatives of catecholamines in cerebrospinal fluid using flow-gated capillary electrophoresis.

PubMed

Zhang, Qiyang; Gong, Maojun

2016-06-10

Flow-gated capillary electrophoresis (CE) coupled with microdialysis has become an important tool for in vivo bioanalytical measurements because it is capable of performing rapid and efficient separations of complex biological mixtures thus enabling high temporal resolution in chemical monitoring. However, the limit of detection (LOD) is often limited to a micro- or nano-molar range while many important target analytes have picomolar or sub-nanomolar levels in brain and other tissues. To enhance the capability of flow-gated CE for catecholamine detection, a novel and simple on-line sample preconcentration method was developed exclusively for fluorescent derivatives of catecholamines that were fluorogenically derivatized with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence of cyanide. The effective preconcentration coupled with the sensitive laser-induced fluorescence (LIF) detection lowered the LOD down to 20pM for norepinephrine (NE) and 50pM for dopamine (DA) at 3-fold of S/N ratio, and the signal enhancement was estimated to be over 100-fold relative to normal injection when standard analytes were dissolved in artificial cerebrospinal fluid (aCSF). The basic focusing principle is novel since the sample plug contains borate while the background electrolyte (BGE) is void of borate. This strategy took advantage of the complexation between diols and borate, through which one negative charge was added to the complex entity. The sample derivatization mixture was electrokinetically injected into a capillary via the flow-gated injection, and then NE and DA derivatives were selectively focused to a narrow zone by the reversible complexation. Separation of NE and DA derivatives was executed by incoming surfactants of cholate and deoxycholate mixed in the front BGE plug. This on-line preconcentration method was finally applied to the detection of DA in rat cerebrospinal fluid (CSF) via microdialysis and on-line derivatization. It is anticipated that the method would

Ultrasonic nebulization extraction-heating gas flow transfer-headspace single drop microextraction of essential oil from pericarp of Zanthoxylum bungeanum Maxim.

PubMed

Wei, Shigang; Zhang, Huihui; Wang, Yeqiang; Wang, Lu; Li, Xueyuan; Wang, Yinghua; Zhang, Hanqi; Xu, Xu; Shi, Yuhua

2011-07-22

The ultrasonic nebulization extraction-heating gas flow transfer coupled with headspace single drop microextraction (UNE-HGFT-HS-SDME) was developed for the extraction of essential oil from Zanthoxylum bungeanum Maxim. The gas chromatography-mass spectrometry was applied to the determination of the constituents in the essential oil. The contents of the constituents from essential oil obtained by the proposed method were found to be more similar to those obtained by hydro-distillation (HD) than those obtained by ultrasonic nebulization extraction coupled with headspace single drop microextraction (UNE-HS-SDME). The heating gas flow was firstly used in the analysis of the essential oil to transfer the analytes from the headspace to the solvent microdrop. The relative standard deviations for determining the five major constituents were in the range from 1.5 to 6.7%. The proposed method is a fast, sensitive, low cost and small sample consumption method for the determination of the volatile and semivolatile constituents in the plant materials. Copyright © 2011 Elsevier B.V. All rights reserved.

Novel Imaging Method of Continuous Shear Wave by Ultrasonic Color Flow Mapping

NASA Astrophysics Data System (ADS)

Yamakoshi, Yoshiki; Yamamoto, Atsushi; Yuminaka, Yasushi

Shear wave velocity measurement is a promising method in evaluation of tissue stiffness. Several methods have been developed to measure the shear wave velocity, however, it is difficult to obtain quantitative shear wave image in real-time by low cost system. In this paper, a novel shear wave imaging method for continuous shear wave is proposed. This method uses a color flow imaging which is used in ultrasonic imaging system to obtain shear wave's wavefront map. Two conditions, shear wave frequency condition and shear wave displacement amplitude condition, are required, however, these conditions are not severe restrictions in most applications. Using the proposed method, shear wave velocity of trapezius muscle is measured. The result is consistent with the velocity which is calculated from shear elastic modulus measured by ARFI method.

Preparation and characterization of lysine-immobilized poly(glycidyl methacrylate) nanoparticle-coated capillary for the separation of amino acids by open tubular capillary electrochromatography.

PubMed

Xu, Liang; Cui, Pengfei; Wang, Dongmei; Tang, Cheng; Dong, Linyi; Zhang, Can; Duan, Hongquan; Yang, Victor C

2014-01-03

In this study, poly(glycidyl methacrylate) (PGMA) nanoparticles (NPs) were prepared and chemically immobilized for the first time onto a capillary inner wall for open tubular capillary electrochromatography (OTCEC). The immobilization of PGMA NPs onto the capillary was attained by a ring-opening reaction between the NPs and an amino-silylated fused capillary inner surface. Scanning electron micrographs clearly demonstrated that the NPs were bound to the capillary inner surface in a dense monolayer. The PGMA NP-coated column was then functionalized by lysine (Lys). After fuctionalization, the capillary can afford strong anodic electroosmotic flow, especially in acidic running buffers. Separations of three amino acids (including tryptophan, tyrosine and phenylalanine) were performed in NP-modified, monolayer Lys-functionalized and bare uncoated capillaries. Results indicated that the NP-coated column can provide more retention and higher resolution for analytes due to the hydrophobic interaction between analytes and the NP-coating. Run-to-run and column-to-column reproducibilities in the separation of the amino acids using the NP-modified column were also demonstrated. Copyright © 2013 Elsevier B.V. All rights reserved.

A model for capillary rise in micro-tube restrained by a sticky layer

NASA Astrophysics Data System (ADS)

Shen, Anqi; Xu, Yun; Liu, Yikun; Cai, Bo; Liang, Shuang; Wang, Fengjiao

2018-06-01

Fluid transport in a microscopic capillary under the effects of a sticky layer was theoretically investigated. A model based on the classical Lucas-Washburn (LW) model is proposed for the meniscus rise with the sticky layer present. The sticky layer consists of two parts: a fixed (located at the wall) and a movable part (located on the inside of the capillary), affecting the micro-capillary flow in different ways. Within our model, the movable layer is defined by the capillary radius and pressure gradient. From the model it follows that the fixed sticky layer leads to a decrease of capillary radius, while the movable sticky layer increases flow resistance. The movable layer thickness varies with the pressure gradient, which in turn varies with the rising of the meniscus. The results of our theoretical calculation also prove that the capillary radius has a greater effect on the meniscus height, rather than the additional resistance caused by the movable layer. Moreover, the fixed sticky layer, which affects the capillary radius, has a greater influence than the movable sticky layer. We conclude that the sticky layer causes a lower imbibition height than the LW model predicts.

Determination of Nitrate Carry-Over on Bytac(registered) Strips Via Capillary Electrophoresis

DTIC Science & Technology

2012-01-19

Beckman Coulter P/ ACE MDQ capillary electrophoresis instrument. A 60 cm long (10 cm effective length), 75 µm i.d. bare fused-silica capillary was used...the separation. Due to the high concentration of the BGE, electroosmotic flow (EOF) is significantly reduced allowing for the application of a...bromide) are not seen in the electropherogram due to the reversed polarity; electroosmotic flow suppression is sufficient to cause the ammonium and

[Characteristics of electroosmotic flow in open-tubular capillary electrochromatography with magnetic nanoparticle coating as mixed-mode stationary phase].

PubMed

Qin, Sasa; Zhou, Chaoran; Zhu, Yaxian; Ren, Zhiyu; Zhang, Lingyi; Fu, Honggang; Zhang, Weibing

2011-09-01

A novel open-tubular capillary electrochromatography (OT-CEC) column with magnetic nanoparticle coating as mixed-mode stationary phase was prepared. The mixed-mode stationary phases were obtained by mixing C18 and amino modified magnetic nanoparticles with different ratios. The mixed modified magnetic nanoparticles as stationary phase were introduced into the capillary by using external magnetic force. The magnetic nanoparticle coating can be easily regenerated by removing the external magnetic field, and applied to other separation modes. The characteristics of electroosmotic flow (EOF) were theoretically investigated through the effect of physicochemical properties of different stationary phases on EOF. The experiment was conducted under different ratios of mixed-mode stationary phases and coating lengths, and it was verified that the theoretical conclusions accorded with the experimental results. It was shown that the EOF can be easily adjusted by changing the ratio of stationary phases or the number of permanent magnets.

Dual-opposite injection capillary electrophoresis: Principles and misconceptions.

PubMed

Blackney, Donna M; Foley, Joe P

2017-03-01

Dual-opposite injection capillary electrophoresis (DOI-CE) is a separation technique that utilizes both ends of the capillary for sample introduction. The electroosmotic flow (EOF) is suppressed to allow all ions to reach the detector quickly. Depending on the individual electrophoretic mobilities of the analytes of interest and the effective length that each analyte travels to the detection window, the elution order of analytes in a DOI-CE separation can vary widely. This review discusses the principles, applications, and limitations of dual-opposite injection capillary electrophoresis. Common misconceptions regarding DOI-CE are clarified. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

IB-LBM simulation of the haemocyte dynamics in a stenotic capillary.

PubMed

Yuan-Qing, Xu; Xiao-Ying, Tang; Fang-Bao, Tian; Yu-Hua, Peng; Yong, Xu; Yan-Jun, Zeng

2014-01-01

To study the behaviour of a haemocyte when crossing a stenotic capillary, the immersed boundary-lattice Boltzmann method was used to establish a quantitative analysis model. The haemocyte was assumed to be spherical and to have an elastic cell membrane, which can be driven by blood flow to adopt a highly deformable character. In the stenotic capillary, the spherical blood cell was stressed both by the flow and the wall dimension, and the cell shape was forced to be stretched to cross the stenosis. Our simulation investigated the haemocyte crossing process in detail. The velocity and pressure were anatomised to obtain information on how blood flows through a capillary and to estimate the degree of cell damage caused by excessive pressure. Quantitative velocity analysis results demonstrated that a large haemocyte crossing a small stenosis would have a noticeable effect on blood flow, while quantitative pressure distribution analysis results indicated that the crossing process would produce a special pressure distribution in the cell interior and to some extent a sudden change between the cell interior and the surrounding plasma.

Cell adhesion during bullet motion in capillaries.

PubMed

Takeishi, Naoki; Imai, Yohsuke; Ishida, Shunichi; Omori, Toshihiro; Kamm, Roger D; Ishikawa, Takuji

2016-08-01

A numerical analysis is presented of cell adhesion in capillaries whose diameter is comparable to or smaller than that of the cell. In contrast to a large number of previous efforts on leukocyte and tumor cell rolling, much is still unknown about cell motion in capillaries. The solid and fluid mechanics of a cell in flow was coupled with a slip bond model of ligand-receptor interactions. When the size of a capillary was reduced, the cell always transitioned to "bullet-like" motion, with a consequent decrease in the velocity of the cell. A state diagram was obtained for various values of capillary diameter and receptor density. We found that bullet motion enables firm adhesion of a cell to the capillary wall even for a weak ligand-receptor binding. We also quantified effects of various parameters, including the dissociation rate constant, the spring constant, and the reactive compliance on the characteristics of cell motion. Our results suggest that even under the interaction between P-selectin glycoprotein ligand-1 (PSGL-1) and P-selectin, which is mainly responsible for leukocyte rolling, a cell is able to show firm adhesion in a small capillary. These findings may help in understanding such phenomena as leukocyte plugging and cancer metastasis. Copyright © 2016 the American Physiological Society.

Development of a low-flow multiplexed interface for capillary electrophoresis/electrospray ion trap mass spectrometry using sequential spray.

PubMed

Chen, Chao-Jung; Li, Fu-An; Her, Guor-Rong

2008-05-01

A multiplexed CE-MS interface using four low-flow sheath liquid ESI sprayers has been developed. Because of the limited space between the low-flow sprayers and the entrance aperture of the ESI source, multichannel analysis is difficult using conventional rotating plate approaches. Instead, a multiplexed low-flow system was achieved by applying an ESI potential sequentially to the four low-flow sprayers, resulting in only one sprayer being sprayed at any given time. The synchronization of the scan event and the voltage relays was accomplished by using the data acquisition signal from the IT mass spectrometer. This synchronization resulted in the ESI voltage being sequentially applied to each of the four sprayers according to the corresponding scan event. With this design, a four-fold increase in analytical throughput was achieved. Because of the use of low-flow interfaces, this multiplexed system has superior sensitivity than a rotating plate design using conventional sheath liquid interfaces. The multiplexed design presented has the potential to be applied to other low-flow multiplexed systems, such as multiplexed capillary LC and multiplexed CEC.

Ultrasensitive Ambient Mass Spectrometric Analysis with a Pin-to-Capillary Flowing Atmospheric-Pressure Afterglow Source

PubMed Central

Shelley, Jacob T.; Wiley, Joshua S.; Hieftje, Gary M.

2011-01-01

The advent of ambient desorption/ionization mass spectrometry has resulted in a strong interest in ionization sources that are capable of direct analyte sampling and ionization. One source that has enjoyed increasing interest is the Flowing Atmospheric-Pressure Afterglow (FAPA). FAPA has been proven capable of directly desorbing/ionizing samples in any phase (solid, liquid, or gas) and with impressive limits of detection (<100 fmol). The FAPA was also shown to be less affected by competitive-ionization matrix effects than other plasma-based sources. However, the original FAPA design exhibited substantial background levels, cluttered background spectra in the negative-ion mode, and significant oxidation of aromatic analytes, which ultimately compromised analyte identification and quantification. In the present study, a change in the FAPA configuration from a pin-to-plate to a pin-to-capillary geometry was found to vastly improve performance. Background signals in positive- and negative-ionization modes were reduced by 89% and 99%, respectively. Additionally, the capillary anode strongly reduced the amount of atomic oxygen that could cause oxidation of analytes. Temperatures of the gas stream that interacts with the sample, which heavily influences desorption capabilities, were compared between the two sources by means of IR thermography. The performance of the new FAPA configuration is evaluated through the determination of a variety of compounds in positive- and negative-ion mode, including agrochemicals and explosives. A detection limit of 4 amol was found for the direct determination of the agrochemical ametryn, and appears to be spectrometer-limited. The ability to quickly screen for analytes in bulk liquid samples with the pin-to-capillary FAPA is also shown. PMID:21627097

Ultrasensitive ambient mass spectrometric analysis with a pin-to-capillary flowing atmospheric-pressure afterglow source.

PubMed

Shelley, Jacob T; Wiley, Joshua S; Hieftje, Gary M

2011-07-15

The advent of ambient desorption/ionization mass spectrometry has resulted in a strong interest in ionization sources that are capable of direct analyte sampling and ionization. One source that has enjoyed increasing interest is the flowing atmospheric-pressure afterglow (FAPA). The FAPA has been proven capable of directly desorbing/ionizing samples in any phase (solid, liquid, or gas) and with impressive limits of detection (<100 fmol). The FAPA was also shown to be less affected by competitive-ionization matrix effects than other plasma-based sources. However, the original FAPA design exhibited substantial background levels, cluttered background spectra in the negative-ion mode, and significant oxidation of aromatic analytes, which ultimately compromised analyte identification and quantification. In the present study, a change in the FAPA configuration from a pin-to-plate to a pin-to-capillary geometry was found to vastly improve performance. Background signals in positive- and negative-ionization modes were reduced by 89% and 99%, respectively. Additionally, the capillary anode strongly reduced the amount of atomic oxygen that could cause oxidation of analytes. Temperatures of the gas stream that interacts with the sample, which heavily influences desorption capabilities, were compared between the two sources by means of IR thermography. The performance of the new FAPA configuration is evaluated through the determination of a variety of compounds in positive- and negative-ion mode, including agrochemicals and explosives. A detection limit of 4 amol was found for the direct determination of the agrochemical ametryn and appears to be spectrometer-limited. The ability to quickly screen for analytes in bulk liquid samples with the pin-to-capillary FAPA is also shown.

Ultrasonic control of ceramic membrane fouling by particles: effect of ultrasonic factors.

PubMed

Chen, Dong; Weavers, Linda K; Walker, Harold W

2006-07-01

Ultrasound at 20 kHz was applied to a cross-flow ultrafiltration system with gamma-alumina membranes in the presence of colloidal silica particles to systematically investigate how ultrasonic factors affect membrane cleaning. Based on imaging of the ultrasonic cavitation region, optimal cleaning occurred when the membrane was outside but close to the cavitation region. Increasing the filtration pressure increased the compressive forces driving cavitation collapse and resulted in fewer cavitation bubbles absorbing and scattering sound waves and increasing sound wave penetration. However, an increased filtration pressure also resulted in greater permeation drag, and subsequently less improvement in permeate flux compared to low filtration pressure. Finally, pulsed ultrasound with short pulse intervals resulted in permeate flux improvement close to that of continuous sonication.

Influence of capillary end effects on steady-state relative permeability estimates from direct pore-scale simulations

NASA Astrophysics Data System (ADS)

Guédon, Gaël Raymond; Hyman, Jeffrey De'Haven; Inzoli, Fabio; Riva, Monica; Guadagnini, Alberto

2017-12-01

We investigate and characterize the influence of capillary end effects on steady-state relative permeabilities obtained in pore-scale numerical simulations of two-phase flows. Our study is motivated by the observation that capillary end effects documented in two-phase laboratory-scale experiments can significantly influence permeability estimates. While numerical simulations of two-phase flows in reconstructed pore-spaces are increasingly employed to characterize relative permeabilities, a phenomenon which is akin to capillary end effects can also arise in such analyses due to the constraints applied at the boundaries of the computational domain. We profile the relative strength of these capillary end effects on the calculation of steady-state relative permeabilities obtained within randomly generated porous micro-structures using a finite volume-based two-phase flow solver. We suggest a procedure to estimate the extent of the regions influenced by these capillary end effects, which in turn allows for the alleviation of bias in the estimation of relative permeabilities.

Local nitric oxide synthase inhibition reduces skeletal muscle glucose uptake but not capillary blood flow during in situ muscle contraction in rats.

PubMed

Ross, Renee M; Wadley, Glenn D; Clark, Michael G; Rattigan, Stephen; McConell, Glenn K

2007-12-01

We have previously shown in humans that local infusion of a nitric oxide synthase (NOS) inhibitor into the femoral artery attenuates the increase in leg glucose uptake during exercise without influencing total leg blood flow. However, rodent studies examining the effect of NOS inhibition on contraction-stimulated skeletal muscle glucose uptake have yielded contradictory results. This study examined the effect of local infusion of an NOS inhibitor on skeletal muscle glucose uptake (2-deoxyglucose) and capillary blood flow (contrast-enhanced ultrasound) during in situ contractions in rats. Male hooded Wistar rats were anesthetized and one hindleg electrically stimulated to contract (2 Hz, 0.1 ms) for 30 min while the other leg rested. After 10 min, the NOS inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME) (arterial concentration of 5 micromol/l) or saline was infused into the epigastric artery of the contracting leg. Local NOS inhibition had no effect on blood pressure, heart rate, or muscle contraction force. Contractions increased (P < 0.05) skeletal muscle NOS activity, and this was prevented by L-NAME infusion. NOS inhibition caused a modest significant (P < 0.05) attenuation of the increase in femoral blood flow during contractions, but importantly there was no effect on capillary recruitment. NOS inhibition attenuated (P < 0.05) the increase in contraction-stimulated skeletal muscle glucose uptake by approximately 35%, without affecting AMP-activated protein kinase (AMPK) activation. NOS inhibition attenuated increases in skeletal muscle glucose uptake during contraction without influencing capillary recruitment, suggesting that NO is critical for part of the normal increase in skeletal muscle fiber glucose uptake during contraction.

Non-invasive classification of gas-liquid two-phase horizontal flow regimes using an ultrasonic Doppler sensor and a neural network

NASA Astrophysics Data System (ADS)

Musa Abbagoni, Baba; Yeung, Hoi

2016-08-01

The identification of flow pattern is a key issue in multiphase flow which is encountered in the petrochemical industry. It is difficult to identify the gas-liquid flow regimes objectively with the gas-liquid two-phase flow. This paper presents the feasibility of a clamp-on instrument for an objective flow regime classification of two-phase flow using an ultrasonic Doppler sensor and an artificial neural network, which records and processes the ultrasonic signals reflected from the two-phase flow. Experimental data is obtained on a horizontal test rig with a total pipe length of 21 m and 5.08 cm internal diameter carrying air-water two-phase flow under slug, elongated bubble, stratified-wavy and, stratified flow regimes. Multilayer perceptron neural networks (MLPNNs) are used to develop the classification model. The classifier requires features as an input which is representative of the signals. Ultrasound signal features are extracted by applying both power spectral density (PSD) and discrete wavelet transform (DWT) methods to the flow signals. A classification scheme of ‘1-of-C coding method for classification’ was adopted to classify features extracted into one of four flow regime categories. To improve the performance of the flow regime classifier network, a second level neural network was incorporated by using the output of a first level networks feature as an input feature. The addition of the two network models provided a combined neural network model which has achieved a higher accuracy than single neural network models. Classification accuracies are evaluated in the form of both the PSD and DWT features. The success rates of the two models are: (1) using PSD features, the classifier missed 3 datasets out of 24 test datasets of the classification and scored 87.5% accuracy; (2) with the DWT features, the network misclassified only one data point and it was able to classify the flow patterns up to 95.8% accuracy. This approach has demonstrated the

A performance comparison of ultrasonically aided electric propulsion extractor configurations

NASA Astrophysics Data System (ADS)

Dong, L.; Song, W.; Kang, X. M.; Zhao, W. S.

2012-08-01

As a novel propulsion technology, ultrasonically aided electric propulsion (UAEP) offers a high specific impulse and a high thrust density. In this paper, the effects of extractor grid configuration on performance of a UAEP thruster have been investigated by both experimental studies and numerical simulation. Relationships between spray current and operation parameters, including applied voltage, propellant flow rate, and vibration power and frequency, are explored for different extractor mesh sizes and shapes. Numerical simulation is also carried out for a better understanding of the formation of capillary standing waves as well as the electric field distribution in the acceleration zone. Experimental results show that compared with a circular shaped extractor, a reticular shaped extractor is able to produce a higher spray current. The current density increases with a denser mesh, which agrees well with the numerical simulation results. This phenomenon indicates that optimizing extractors with appropriate shapes and sizes can be an effective way to improve the performance of a UAEP system. A performance evaluation based on hydrodynamic and electrostatic calculations indicates that the present UAEP system can produce a thrust competitive to that of the colloid thruster with an emitter array.

A Capillary-Based Static Phase Separator for Highly Variable Wetting Conditions

NASA Technical Reports Server (NTRS)

Thomas, Evan A.; Graf, John C.; Weislogel, Mark M.

2010-01-01

The invention, a static phase separator (SPS), uses airflow and capillary wetting characteristics to passively separate a two-phase (liquid and air) flow. The device accommodates highly variable liquid wetting characteristics. The resultant design allows for a range of wetting properties from about 0 to over 90 advancing contact angle, with frequent complete separation of liquid from gas observed when using appropriately scaled test conditions. Additionally, the design accommodates a range of air-to-liquid flow-rate ratios from only liquid flow to over 200:1 air-to-liquid flow rate. The SPS uses a helix input section with an ice-cream-cone-shaped constant area cross section (see figure). The wedge portion of the cross section is on the outer edge of the helix, and collects the liquid via centripetal acceleration. The helix then passes into an increasing cross-sectional area vane region. The liquid in the helix wedge is directed into the top of capillary wedges in the liquid containment section. The transition from diffuser to containment section includes a 90 change in capillary pumping direction, while maintaining inertial direction. This serves to impinge the liquid into the two off-center symmetrical vanes by the airflow. Rather than the airflow serving to shear liquid away from the capillary vanes, the design allows for further penetration of the liquid into the vanes by the air shear. This is also assisted by locating the air exit ports downstream of the liquid drain port. Additionally, any droplets not contained in the capillary vanes are re-entrained downstream by a third opposing capillary vane, which directs liquid back toward the liquid drain port. Finally, the dual air exit ports serve to slow the airflow down, and to reduce the likelihood of shear. The ports are stove-piped into the cavity to form an unfriendly capillary surface for a wetting fluid to carryover. The liquid drain port is located at the start of the containment region, allowing for

Blood Perfusion in Microfluidic Models of Pulmonary Capillary Networks: Role of Geometry and Hematocrit

NASA Astrophysics Data System (ADS)

Stauber, Hagit; Waisman, Dan; Sznitman, Josue; Technion-IIT Team; Department of Neonatology Carmel Medical Center; Faculty of Medicine-Technion IIT Collaboration

2015-11-01

Microfluidic platforms are increasingly used to study blood microflows at true physiological scale due to their ability to overcome manufacturing obstacle of complex anatomical morphologies, such as the organ-specific architectures of the microcirculation. In the present work, we utilize microfluidic platforms to devise in vitro models of the underlying pulmonary capillary networks (PCN), where capillary lengths and diameters are similar to the size of RBCs (~ 5-10 μm). To better understand flow characteristics and dispersion of red blood cells (RBCs) in PCNs, we have designed microfluidic models of alveolar capillary beds inspired by the seminal ``sheet flow'' model of Fung and Sobin (1969). Our microfluidic PCNs feature confined arrays of staggered pillars with diameters of ~ 5,7 and 10 μm, mimicking the dense structure of pulmonary capillary meshes. The devices are perfused with suspensions of RBCs at varying hematocrit levels under different flow rates. Whole-field velocity patterns using micro-PIV and single-cell tracking using PTV are obtained with fluorescently-labelled RBCs and discussed. Our experiments deliver a real-scale quantitative description of RBC perfusion characteristics across the pulmonary capillary microcirculation.

The relationship between human placental morphometry and ultrasonic measurements of utero-placental blood flow and fetal growth.

PubMed

Salavati, N; Sovio, U; Mayo, R Plitman; Charnock-Jones, D S; Smith, G C S

2016-02-01

Ultrasonic fetal biometry and arterial Doppler flow velocimetry are widely used to assess the risk of pregnancy complications. There is an extensive literature on the relationship between pregnancy outcomes and the size and shape of the placenta. However, ultrasonic fetal biometry and arterial Doppler flow velocimetry have not previously been studied in relation to postnatal placental morphometry in detail. We conducted a prospective cohort study of nulliparous women in The Rosie Hospital, Cambridge (UK). We studied a group of 2120 women who had complete data on uterine and umbilical Doppler velocimetry and fetal biometry at 20, 28 and 36 weeks' gestational age, digital images of the placenta available, and delivered a liveborn infant at term. Associations were expressed as the difference in the standard deviation (SD) score of the gestational age adjusted ultrasound measurement (z-score) comparing the lowest and highest decile of the given placental morphometric measurement. The lowest decile of placental surface area was associated with 0.87 SD higher uterine artery Doppler mean pulsatility index (PI) at 20 weeks (95% CI: 0.68 to 1.07, P < 0.001). The lowest decile of placental weight was associated with 0.73 SD higher umbilical artery Doppler PI at 36 weeks (95% CI: 0.54 to 0.93, P < 0.001). The lowest decile of both placental weight and placental area were associated with reduced growth velocity of the fetal abdominal circumference between 20 and 36 weeks (both P < 0.001). Placental area and weight are associated with uterine and umbilical blood flow, respectively, and both are associated with fetal growth rate. Copyright © 2015 Elsevier Ltd. All rights reserved.

A novel in situ strategy for the preparation of a β-cyclodextrin/polydopamine-coated capillary column for capillary electrochromatography enantioseparations.

PubMed

Guo, Heying; Niu, Xiaoying; Pan, Congjie; Yi, Tao; Chen, Hongli; Chen, Xingguo

2017-06-01

Inspired by the chiral recognition ability of β-cyclodextrin and the natural adhesive properties of polydopamine under alkaline conditions, in this study, a rapid and in situ modification strategy was developed to fabricate β-cyclodextrin/polydopamine composite material coated-capillary columns for open tubular capillary electrochromatography. The results of scanning electron microscopy, FTIR spectroscopy, streaming potential, and electro-osmotic flow studies indicated that β-cyclodextrin/polydopamine was successfully fixed on the inner wall of the capillary column. This coating can be achieved within 1 h affording a greatly reduced capillary preparation time. The performance of the β-cyclodextrin/polydopamine-coated capillary was validated by the analysis of seven pairs of chiral analytes, namely epinephrine, norepinephrine, isoprenaline, terbutaline, verapamil, tryptophane, carvedilol. Good enantioseparation efficiencies were achieved for all. For three consecutive runs, the relative standard deviations for the migration times of the analytes for intraday, interday, and column-to-column repeatability were in the range of 0.41-1.74, 1.03-4.18, and 1.66-8.24%, respectively. Moreover, the separation efficiency of the β-cyclodextrin/polydopamine-coated capillary column did not decrease obviously over 90 runs. The strategy should also be feasible to introduce and immobilize other chiral selectors on the inner walls surface of capillary columns. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

A compact and low-cost laser induced fluorescence detector with silicon based photodetector assembly for capillary flow systems.

PubMed

Geng, Xuhui; Shi, Meng; Ning, Haijing; Feng, Chunbo; Guan, Yafeng

2018-05-15

A compact and low-cost laser induced fluorescence (LIF) detector based on confocal structure for capillary flow systems was developed and applied for analysis of Her2 protein on single Hela cells. A low-power and low-cost 450 nm laser diode (LD) instead of a high quality laser was used as excitation light source. A compact optical design together with shortened optical path length improved the optical efficiency and detection sensitivity. A superior silicon based photodetector assembly was used for fluorescence detection instead of a photomultiplier (PMT). The limit of detection (LOD) for fluorescein sodium was 3 × 10 -12 M or 165 fluorescein molecules in detection volume measured on a homemade capillary electroosmotic driven (EOD)-LIF system, which was similar to commercial LIFs. Compared to commercial LIFs, the whole volume of our LIF was reduced to 1/2-1/3, and the cost was less than 1/3 of them. Copyright © 2018 Elsevier B.V. All rights reserved.

Enhancing separation in short-capillary electrophoresis via pressure-driven backflow.

PubMed

Tian, Miaomiao; Wang, Yujia; Mohamed, Amara Camara; Guo, Liping; Yang, Li

2015-07-01

We present a novel easy-to-operate and efficient method to improve the separation efficiency in short-capillary electrophoresis by introducing steady backflow to counterbalance electro-osmotic flow without the use of any external pressure. The backflow was easily generated by tapering the capillary end, which was achieved by heating a straight capillary and stretching it with a constant force. We investigated the net fluidic transport rate under different tip lengths and separation voltages. Good run-to-run repeatability and capillary-to-capillary reproducibility of the present method were obtained with RSD less than 1.5%, indicating the stability of the fluid transport rate in the tapered capillary, which ensures the quantification and repeatability of capillary zone electrophoresis (CZE) analysis. Enhanced separation of the tapered short capillary electrophoresis was demonstrated by CZE analyzing amino acids and positional isomers. Baseline separations were achieved in less than 60 s using a tapered capillary with the effective length of 5 cm, while no separation was achieved using a normal capillary without a tapered tip. The present study provides a promising method to use pressure-driven backflow to enhance separation efficiency in short-capillary electrophoresis, which would be of potential value in a wide application for fast analysis of complex samples. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Thromboxane plays a role in postprandial jejunal oxygen uptake and capillary exchange.

PubMed

Alemayehu, A; Chou, C C

1990-09-01

The effects of a thromboxane A2 (TxA2)-endoperoxide receptor antagonist, SQ 29548, on jejunal blood flow, oxygen uptake, and capillary filtration coefficient (Kfc) were determined in anesthetized dogs under resting conditions and during the presence of predigested food in the jejunal lumen in three series of experiments. In series 1, 2.0 micrograms intra-arterial administration of SQ 29548 was found to abolish completely the vasoconstrictor action of graded doses (0.05-2.0 micrograms) of intra-arterial injection of a TxA2-endoperoxide analogue, U44069. SQ 29548 (2.0 micrograms ia) per se did not significantly alter resting jejunal blood flow, oxygen uptake, capillary pressure, or Kfc. Before SQ 29548, placement of food plus bile into the jejunal lumen increased blood flow +42 +/- 9%, oxygen uptake +28 +/- 7%, and Kfc +24 +/- 6%. After SQ 29548, the food placement increased blood flow +37 +/- 8%, oxygen uptake +52 +/- 11%, and Kfc +63 +/- 20%. The food-induced increases in oxygen uptake and Kfc after SQ 29548 were significantly greater than those induced before the blocking of TxA2-endoperoxide receptors by SQ 29548. Our study indicates that endogenous thromboxane does not play a role in regulating jejunal blood flow, capillary filtration, and oxygen uptake under resting conditions. However, it plays a role in limiting the food-induced increases in jejunal oxygen uptake and capillary exchange capacity without influencing the food-induced hyperemia.

SPH-DEM approach to numerically simulate the deformation of three-dimensional RBCs in non-uniform capillaries.

PubMed

Polwaththe-Gallage, Hasitha-Nayanajith; Saha, Suvash C; Sauret, Emilie; Flower, Robert; Senadeera, Wijitha; Gu, YuanTong

2016-12-28

Blood continuously flows through the blood vessels in the human body. When blood flows through the smallest blood vessels, red blood cells (RBCs) in the blood exhibit various types of motion and deformed shapes. Computational modelling techniques can be used to successfully predict the behaviour of the RBCs in capillaries. In this study, we report the application of a meshfree particle approach to model and predict the motion and deformation of three-dimensional RBCs in capillaries. An elastic spring network based on the discrete element method (DEM) is employed to model the three-dimensional RBC membrane. The haemoglobin in the RBC and the plasma in the blood are modelled as smoothed particle hydrodynamics (SPH) particles. For validation purposes, the behaviour of a single RBC in a simple shear flow is examined and compared against experimental results. Then simulations are carried out to predict the behaviour of RBCs in a capillary; (i) the motion of five identical RBCs in a uniform capillary, (ii) the motion of five identical RBCs with different bending stiffness (K b ) values in a stenosed capillary, (iii) the motion of three RBCs in a narrow capillary. Finally five identical RBCs are employed to determine the critical diameter of a stenosed capillary. Validation results showed a good agreement with less than 10% difference. From the above simulations, the following results are obtained; (i) RBCs exhibit different deformation behaviours due to the hydrodynamic interaction between them. (ii) Asymmetrical deformation behaviours of the RBCs are clearly observed when the bending stiffness (K b ) of the RBCs is changed. (iii) The model predicts the ability of the RBCs to squeeze through smaller blood vessels. Finally, from the simulations, the critical diameter of the stenosed section to stop the motion of blood flow is predicted. A three-dimensional spring network model based on DEM in combination with the SPH method is successfully used to model the motion and

The capillary bed offers the largest hemodynamic resistance to the cortical blood supply

PubMed Central

Gould, Ian Gopal; Tsai, Philbert; Kleinfeld, David

2016-01-01

The cortical angioarchitecture is a key factor in controlling cerebral blood flow and oxygen metabolism. Difficulties in imaging the complex microanatomy of the cortex have so far restricted insight about blood flow distribution in the microcirculation. A new methodology combining advanced microscopy data with large scale hemodynamic simulations enabled us to quantify the effect of the angioarchitecture on the cerebral microcirculation. High-resolution images of the mouse primary somatosensory cortex were input into with a comprehensive computational model of cerebral perfusion and oxygen supply ranging from the pial vessels to individual brain cells. Simulations of blood flow, hematocrit and oxygen tension show that the wide variation of hemodynamic states in the tortuous, randomly organized capillary bed is responsible for relatively uniform cortical tissue perfusion and oxygenation. Computational analysis of microcirculatory blood flow and pressure drops further indicates that the capillary bed, including capillaries adjacent to feeding arterioles (d < 10 µm), are the largest contributors to hydraulic resistance. PMID:27780904

Ultrasonic flow metering system

DOEpatents

Gomm, Tyler J.; Kraft, Nancy C.; Mauseth, Jason A.; Phelps, Larry D.; Taylor, Steven C.

2002-01-01

A system for determining the density, flow velocity, and mass flow of a fluid comprising at least one sing-around circuit that determines the velocity of a signal in the fluid and that is correlatable to a database for the fluid. A system for determining flow velocity uses two of the inventive circuits with directional transmitters and receivers, one of which is set at an angle to the direction of flow that is different from the others.

A capillary-driven micromixer: idea and fabrication

NASA Astrophysics Data System (ADS)

Lee, Chun-Te; Lee, Chun-Che

2012-10-01

Microfluidic systems have been drawing attention upon the various branches of engineering science and the allied areas within biology and biomedicine. In this paper, a fabrication of a capillary-driven micromixer using photoresist JSR and glasses is proposed. We design three types of planar capillary-driven micormixers with different sizes of baffles in the channel. Flow tests have shown that the micromixer with a baffle gap of 100 μm and space of 100 μm reaches a best mixing performance of 93% in gray-level image analysis.

Electroosmotic flow in capillary channels filled with nonconstant viscosity electrolytes: exact solution of the Navier-Stokes equation.

PubMed

Otevrel, Marek; Klepárník, Karel

2002-10-01

The partial differential equation describing unsteady velocity profile of electroosmotic flow (EOF) in a cylindrical capillary filled with a nonconstant viscosity electrolyte was derived. Analytical solution, based on the general Navier-Stokes equation, was found for constant viscosity electrolytes using the separation of variables (Fourier method). For the case of a nonconstant viscosity electrolyte, the steady-state velocity profile was calculated assuming that the viscosity decreases exponentially in the direction from the wall to the capillary center. Since the respective equations with nonconstant viscosity term are not solvable in general, the method of continuous binding conditions was used to solve this problem. In this method, an arbitrary viscosity profile can be modeled. The theoretical conclusions show that the relaxation times at which an EOF approaches the steady state are too short to have an impact on a separation process in any real systems. A viscous layer at the wall affects EOF significantly, if it is thicker than the Debye length of the electric double layer. The presented description of the EOF dynamics is applicable to any microfluidic systems.

21 CFR 892.1540 - Nonfetal ultrasonic monitor.

Code of Federal Regulations, 2012 CFR

2012-04-01

...) Identification. A nonfetal ultrasonic monitor is a device that projects a continuous high-frequency sound wave... wave and is intended for use in the investigation of nonfetal blood flow and other nonfetal body...

21 CFR 892.1540 - Nonfetal ultrasonic monitor.

Code of Federal Regulations, 2013 CFR

2013-04-01

...) Identification. A nonfetal ultrasonic monitor is a device that projects a continuous high-frequency sound wave... wave and is intended for use in the investigation of nonfetal blood flow and other nonfetal body...

Molecular transport through capillaries made with atomic-scale precision

NASA Astrophysics Data System (ADS)

Radha, B.; Esfandiar, A.; Wang, F. C.; Rooney, A. P.; Gopinadhan, K.; Keerthi, A.; Mishchenko, A.; Janardanan, A.; Blake, P.; Fumagalli, L.; Lozada-Hidalgo, M.; Garaj, S.; Haigh, S. J.; Grigorieva, I. V.; Wu, H. A.; Geim, A. K.

2016-10-01

Nanometre-scale pores and capillaries have long been studied because of their importance in many natural phenomena and their use in numerous applications. A more recent development is the ability to fabricate artificial capillaries with nanometre dimensions, which has enabled new research on molecular transport and led to the emergence of nanofluidics. But surface roughness in particular makes it challenging to produce capillaries with precisely controlled dimensions at this spatial scale. Here we report the fabrication of narrow and smooth capillaries through van der Waals assembly, with atomically flat sheets at the top and bottom separated by spacers made of two-dimensional crystals with a precisely controlled number of layers. We use graphene and its multilayers as archetypal two-dimensional materials to demonstrate this technology, which produces structures that can be viewed as if individual atomic planes had been removed from a bulk crystal to leave behind flat voids of a height chosen with atomic-scale precision. Water transport through the channels, ranging in height from one to several dozen atomic planes, is characterized by unexpectedly fast flow (up to 1 metre per second) that we attribute to high capillary pressures (about 1,000 bar) and large slip lengths. For channels that accommodate only a few layers of water, the flow exhibits a marked enhancement that we associate with an increased structural order in nanoconfined water. Our work opens up an avenue to making capillaries and cavities with sizes tunable to ångström precision, and with permeation properties further controlled through a wide choice of atomically flat materials available for channel walls.

Wicking Tests for Unidirectional Fabrics: Measurements of Capillary Parameters to Evaluate Capillary Pressure in Liquid Composite Molding Processes.

PubMed

Pucci, Monica Francesca; Liotier, Pierre-Jacques; Drapier, Sylvain

2017-01-27

During impregnation of a fibrous reinforcement in liquid composite molding (LCM) processes, capillary effects have to be understood in order to identify their influence on void formation in composite parts. Wicking in a fibrous medium described by the Washburn equation was considered equivalent to a flow under the effect of capillary pressure according to the Darcy law. Experimental tests for the characterization of wicking were conducted with both carbon and flax fiber reinforcement. Quasi-unidirectional fabrics were then tested by means of a tensiometer to determine the morphological and wetting parameters along the fiber direction. The procedure was shown to be promising when the morphology of the fabric is unchanged during capillary wicking. In the case of carbon fabrics, the capillary pressure can be calculated. Flax fibers are sensitive to moisture sorption and swell in water. This phenomenon has to be taken into account to assess the wetting parameters. In order to make fibers less sensitive to water sorption, a thermal treatment was carried out on flax reinforcements. This treatment enhances fiber morphological stability and prevents swelling in water. It was shown that treated fabrics have a linear wicking trend similar to those found in carbon fabrics, allowing for the determination of capillary pressure.

Spontaneous Imbibition Process in Micro-Nano Fractal Capillaries Considering Slip Flow

NASA Astrophysics Data System (ADS)

Shen, Yinghao; Li, Caoxiong; Ge, Hongkui; Guo, Xuejing; Wang, Shaojun

An imbibition process of water into a matrix is required to investigate the influences of large-volume fracturing fluids on gas production of unconventional formations. Slip flow has been recognized by recent studies as a major mechanism of fluid transport in nanotubes. For nanopores in shale, a slip boundary is nonnegligible in the imbibition process. In this study, we established an analytic equation of spontaneous imbibition considering slip effects in capillaries. A spontaneous imbibition model that couples the analytic equation considering the slip effect was constructed based on fractal theory. We then used a model for various conditions, such as slip boundary, pore structure, and fractal dimension of pore tortuosity, to capture the imbibition characteristics considering the slip effect. A dynamic contact angle was integrated into the modeling. Results of our study verify that the slip boundary influences water imbibition significantly. The imbibition speed is significantly improved when slip length reaches the equivalent diameter of a tube. Therefore, disregarding the slip effect will underestimate the imbibition speed in shale samples.

Polymethylmethacrylate (PMMA) Material Test Results for the Capillary Flow Experiments (CFE)

NASA Technical Reports Server (NTRS)

Lerch, Bradley A.; Thesken, John C.; Bunnell, Charles T.

2007-01-01

In support of the Capillary Flow Experiments (CFE) program, several polymethylmethacrylate (PMMA) flight vessels were constructed. Some vessels used a multipiece design, which was chemically welded together. Due to questions regarding the effects of the experiment fluid (silicone oil) on the weld integrity, a series of tests were conducted to provide evidence of the adequacy of the current vessel design. Tensile tests were conducted on PMMA samples that were both in the as-received condition, and also aged in air or oil for up to 8 weeks. Both welded and unwelded samples were examined. Fracture of the joints was studied using notched tensile specimens and Brazilian disk tests. Results showed that aging had no effect on tensile properties. While the welded samples were weaker than the base parent material, the weld strength was found to be further degraded by bubbles in the weld zone. Finally a fracture analysis using the worst-case fracture conditions of the vessel was performed, and the vessel design was found to have a factor of three safety margin.

Synchrotron radiation microtomography of Taylor bubbles in capillary two-phase flow

NASA Astrophysics Data System (ADS)

Boden, Stephan; dos Santos Rolo, Tomy; Baumbach, Tilo; Hampel, Uwe

2014-07-01

We report on a study to measure the three-dimensional shape of Taylor bubbles in capillaries using synchrotron radiation in conjunction with ultrafast radiographic imaging. Moving Taylor bubbles in 2-mm round and square capillaries were radiographically scanned with an ultrahigh frame rate of up to 36,000 fps and 5.6-µm pixel separation. Consecutive images were properly processed to yield 2D transmission radiographs of high contrast-to-noise ratio. Application of 3D tomographic image reconstruction disclosed the 3D bubble shape. The results provide a reference data base for development of sophisticated interface resolving CFD computations.

The effect of intralesional steroid injections on the volume and blood flow in periocular capillary haemangiomas.

PubMed

Verity, David H; Rose, Geoffrey E; Restori, M

2008-01-01

To examine the effect of steroid therapy on the volume estimates and blood flow characteristics of childhood periorbital capillary haemangiomas. Children at risk of amblyopia due to periorbital haemangiomas were treated with intralesional steroid injections (between 1 and 4 courses) and serial assessment of the volume and blood-flow characteristics of the lesions measured using colour Doppler ultrasonography. The characteristics of the haemangiomas in these children were compared with a cohort of untreated cases. Eight of nine treated children were female, this proportion being significantly different from the equal sex distribution of an untreated cohort (p < 0.05). All children in the steroid-treated group presented within 1 month of birth, compared to the untreated children, who presented at an average of 2.1 months of age (range 0-14, median 2.9 months) (p = 0.04) and they required significantly longer follow-up in the Orbital service (mean 65 months, range 26-105), compared with an average of 35 months (range 4-92, median 23) in the untreated group (p = 0.002). The maximum estimated volume of the lesions were significantly larger in the treated group (treated group mean 8.9 ml, untreated group mean 4.1 ml; p = 0.016), with a trend towards higher maximum measured blood velocities in the treated group (treated mean 64 cm compared with untreated mean 52 cm; p = 0.1). Steroid injections appear to reduce the volume and blood flow of haemangiomas, this suppression persisting for several months (between 5 and 20) before the lesion later displays the cyclic fluctuations in volume and flow seen with untreated lesions. All treated haemangiomas had some residual vascular anomaly, detectable on ultrasonography, at last follow-up--this being despite absence of clinical signs in most cases. Periorbital capillary haemangiomas requiring steroid therapy for risk of amblyopia were significantly commoner in females, were larger lesions and presented at an earlier age

Automated two-dimensional interface for capillary gas chromatography

DOEpatents

Strunk, M.R.; Bechtold, W.E.

1996-02-20

A multidimensional gas chromatograph (GC) system is disclosed which has wide bore capillary and narrow bore capillary GC columns in series and has a novel system interface. Heart cuts from a high flow rate sample, separated by a wide bore GC column, are collected and directed to a narrow bore GC column with carrier gas injected at a lower flow compatible with a mass spectrometer. A bimodal six-way valve is connected with the wide bore GC column outlet and a bimodal four-way valve is connected with the narrow bore GC column inlet. A trapping and retaining circuit with a cold trap is connected with the six-way valve and a transfer circuit interconnects the two valves. The six-way valve is manipulated between first and second mode positions to collect analyte, and the four-way valve is manipulated between third and fourth mode positions to allow carrier gas to sweep analyte from a deactivated cold trap, through the transfer circuit, and then to the narrow bore GC capillary column for separation and subsequent analysis by a mass spectrometer. Rotary valves have substantially the same bore width as their associated columns to minimize flow irregularities and resulting sample peak deterioration. The rotary valves are heated separately from the GC columns to avoid temperature lag and resulting sample deterioration. 3 figs.

Automated two-dimensional interface for capillary gas chromatography

DOEpatents

Strunk, Michael R.; Bechtold, William E.

1996-02-20

A multidimensional gas chromatograph (GC) system having wide bore capillary and narrow bore capillary GC columns in series and having a novel system interface. Heart cuts from a high flow rate sample, separated by a wide bore GC column, are collected and directed to a narrow bore GC column with carrier gas injected at a lower flow compatible with a mass spectrometer. A bimodal six-way valve is connected with the wide bore GC column outlet and a bimodal four-way valve is connected with the narrow bore GC column inlet. A trapping and retaining circuit with a cold trap is connected with the six-way valve and a transfer circuit interconnects the two valves. The six-way valve is manipulated between first and second mode positions to collect analyte, and the four-way valve is manipulated between third and fourth mode positions to allow carrier gas to sweep analyte from a deactivated cold trap, through the transfer circuit, and then to the narrow bore GC capillary column for separation and subsequent analysis by a mass spectrometer. Rotary valves have substantially the same bore width as their associated columns to minimize flow irregularities and resulting sample peak deterioration. The rotary valves are heated separately from the GC columns to avoid temperature lag and resulting sample deterioration.

Numerical Modeling of Interstitial Fluid Flow Coupled with Blood Flow through a Remodeled Solid Tumor Microvascular Network

PubMed Central

Soltani, M.; Chen, P.

2013-01-01

Modeling of interstitial fluid flow involves processes such as fluid diffusion, convective transport in extracellular matrix, and extravasation from blood vessels. To date, majority of microvascular flow modeling has been done at different levels and scales mostly on simple tumor shapes with their capillaries. However, with our proposed numerical model, more complex and realistic tumor shapes and capillary networks can be studied. Both blood flow through a capillary network, which is induced by a solid tumor, and fluid flow in tumor’s surrounding tissue are formulated. First, governing equations of angiogenesis are implemented to specify the different domains for the network and interstitium. Then, governing equations for flow modeling are introduced for different domains. The conservation laws for mass and momentum (including continuity equation, Darcy’s law for tissue, and simplified Navier–Stokes equation for blood flow through capillaries) are used for simulating interstitial and intravascular flows and Starling’s law is used for closing this system of equations and coupling the intravascular and extravascular flows. This is the first study of flow modeling in solid tumors to naturalistically couple intravascular and extravascular flow through a network. This network is generated by sprouting angiogenesis and consisting of one parent vessel connected to the network while taking into account the non-continuous behavior of blood, adaptability of capillary diameter to hemodynamics and metabolic stimuli, non-Newtonian blood flow, and phase separation of blood flow in capillary bifurcation. The incorporation of the outlined components beyond the previous models provides a more realistic prediction of interstitial fluid flow pattern in solid tumors and surrounding tissues. Results predict higher interstitial pressure, almost two times, for realistic model compared to the simplified model. PMID:23840579

Study on interfacial stability and internal flow of a droplet levitated by ultrasonic wave.

PubMed

Abe, Yutaka; Yamamoto, Yuji; Hyuga, Daisuke; Awazu, Shigeru; Aoki, Kazuyoshi

2009-04-01

For a microgravity environment, new and high-quality material is expected to be manufactured. However, the effect of surface instability and the internal flow become significant when the droplet becomes large. Elucidation of internal flow and surface instability on a levitated droplet is required for the quality improvement of new material manufacturing in a microgravity environment. The objectives of this study are to clarify the interfacial stability and internal flow of a levitated droplet. Surface instability and internal flow are investigated with a large droplet levitated by the ultrasonic acoustic standing wave. The experiment with a large droplet is conducted both under normal gravity and microgravity environments. In the experiment, at first, the characteristics of the levitated droplet are investigated; that is, the relationships among the levitated droplet diameter, the droplet aspect ratio, the displacement of the antinode of the standing wave, and the sound pressure are experimentally measured. As a result, it is clarified that the levitated droplet tends to be located at an optimal position with an optimal shape and diameter. Second, the border condition between the stable and the unstable levitation of the droplet is evaluated by using the existing stability theory. The experimental results qualitatively agree with the theory. It is suggested that the stability of the droplet can be evaluated with the stability theory. Finally, multidimensional visual measurement is conducted to investigate the internal flow structure in a levitated droplet. It is suggested that complex flow with the vortex is generated in the levitated droplet. Moreover, the effect of physical properties of the test fluid on the internal flow structure of the levitated droplet is investigated. As a result, the internal flow structure of the levitated droplet is affected by the surface tension and viscosity.

Multiplexed detection of nitrate and nitrite for capillary electrophoresis with an automated device for high injection efficiency.

PubMed

Gao, Leyi; Patterson, Eric E; Shippy, Scott A

2006-02-01

A simple automated nanoliter scale injection device which allows for reproducible 5 nL sample injections from samples with a volume of <1 microL is successfully used for conventional capillary electrophoresis (CE) and Hadamard transform (HT) CE detection. Two standard fused silica capillaries are assembled axially through the device to function as an injection and a separation capillary. Sample solution is supplied to the injection capillary using pressure controlled with a solenoid valve. Buffer solution flows gravimetrically by the junction of the injection and separation capillaries and is also gated with a solenoid valve. Plugs of sample are pushed into the space between the injection and separation capillaries for electrokinectic injection. To evaluate the performance of the injection device, several optimizations are performed including the influence of flow rates, the injected sample volume and the control of the buffer transverse flow on the overall sensitivity. The system was then applied to HT-CE-UV detection for the signal-to-noise ratio (S/N) improvement of the nitric oxide (NO) metabolites, nitrite and nitrate. In addition, signal averaging was performed to explore the possibility of greater sensitivity enhancements compared to single injections.

Experimental investigation of conical bubble structure and acoustic flow structure in ultrasonic field.

PubMed

Ma, Xiaojian; Huang, Biao; Wang, Guoyu; Zhang, Mindi

2017-01-01

The objective of this paper is to investigate the transient conical bubble structure (CBS) and acoustic flow structure in ultrasonic field. In the experiment, the high-speed video and particle image velocimetry (PIV) techniques are used to measure the acoustic cavitation patterns, as well as the flow velocity and vorticity fields. Results are presented for a high power ultrasound with a frequency of 18kHz, and the range of the input power is from 50W to 250W. The results of the experiment show the input power significantly affects the structures of CBS, with the increase of input power, the cavity region of CBS and the velocity of bubbles increase evidently. For the transient motion of bubbles on radiating surface, two different types could be classified, namely the formation, aggregation and coalescence of cavitation bubbles, and the aggregation, shrink, expansion and collapse of bubble cluster. Furthermore, the thickness of turbulent boundary layer near the sonotrode region is found to be much thicker, and the turbulent intensities are much higher for relatively higher input power. The vorticity distribution is prominently affected by the spatial position and input power. Copyright © 2016 Elsevier B.V. All rights reserved.

Capillary electrophoresis for drug analysis

NASA Astrophysics Data System (ADS)

Lurie, Ira S.

1999-02-01

Capillary electrophoresis (CE) is a high resolution separation technique which is amenable to a wide variety of solutes, including compounds which are thermally degradable, non-volatile and highly polar, and is therefore well suited for drug analysis. Techniques which have been used in our laboratory include electrokinetic chromatography (ECC), free zone electrophoresis (CZE) and capillary electrochromatography (CEC). ECC, which uses a charged run buffer additive which migrates counter to osmotic flow, is excellent for many applications, including, drug screening and analyses of heroin, cocaine and methamphetamine samples. ECC approaches include the use of micelles and charged cyclodextrins, which allow for the separation of complex mixtures. Simultaneous separation of acidic, neutral and basic solutes and the resolution of optical isomers and positional isomers are possible. CZE has been used for the analysis of small ions (cations and anions) in heroin exhibits. For the ECC and CZE experiments performed in our laboratory, uncoated capillaries were used. In contrast, CEC uses capillaries packed with high performance liquid chromatography stationary phases, and offers both high peak capacities and unique selectivities. Applications include the analysis of cannabinoids and drug screening. Although CE suffers from limited concentration sensitivity, it is still applicable to trace analysis of drug samples, especially when using injection techniques such as stacking, or detection schemes such as laser induced fluorescence and extended pathlength UV.

Capillary Structures for Exploration Life Support (Capillary Structures)

NASA Image and Video Library

2017-07-10

iss052e013146 (July 10, 2017) --- Astronaut Jack Fischer is photographed during setup of hardware for the Capillary Structures for Exploration Life Support (Capillary Structures) two sorbent demonstrations. The Capillary Structures for Exploration Life Support (Capillary Structures) investigation studies a new method using structures of specific shapes to manage fluid and gas mixtures. The investigation studies water recycling and carbon dioxide removal, benefiting future efforts to design lightweight, more reliable life support systems for future space missions.

Effects of intermediate wettability on entry capillary pressure in angular pores.

PubMed

Rabbani, Harris Sajjad; Joekar-Niasar, Vahid; Shokri, Nima

2016-07-01

Entry capillary pressure is one of the most important factors controlling drainage and remobilization of the capillary-trapped phases as it is the limiting factor against the two-phase displacement. It is known that the entry capillary pressure is rate dependent such that the inertia forces would enhance entry of the non-wetting phase into the pores. More importantly the entry capillary pressure is wettability dependent. However, while the movement of a meniscus into a strongly water-wet pore is well-defined, the invasion of a meniscus into a weak or intermediate water-wet pore especially in the case of angular pores is ambiguous. In this study using OpenFOAM software, high-resolution direct two-phase flow simulations of movement of a meniscus in a single capillary channel are performed. Interface dynamics in angular pores under drainage conditions have been simulated under constant flow rate boundary condition at different wettability conditions. Our results shows that the relation between the half corner angle of pores and contact angle controls the temporal evolution of capillary pressure during the invasion of a pore. By deviating from pure water-wet conditions, a dip in the temporal evolution of capillary pressure can be observed which will be pronounced in irregular angular cross sections. That enhances the pore invasion with a smaller differential pressure. The interplay between the contact angle and pore geometry can have significant implications for enhanced remobilization of ganglia in intermediate contact angles in real porous media morphologies, where pores are very heterogeneous with small shape factors. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Measurement of pulmonary capillary blood flow in infants by plethysmography.

PubMed Central

Stocks, J; Costeloe, K; Winlove, C P; Godfrey, S

1977-01-01

An accurate method for measuring effective pulmonary capillary blood flow (Qc eff) in infants has been developed with an adaptation of the plethysmographic technique. Measurements were made on 19 preterm. 14 small-for-dates, and 7 fullterm normal infants with a constant volume whole body plethysmograph in which the infant rebreathed nitrous oxide. There was a highly significant correlation between Qc eff and body weight, and this relationship was unaffected by premature delivery or intrauterine growth retardation. Mean Qc eff in preterm, small-for dates, and fullterm infants was 203, 208 and 197 ml min-1 kg-1, respectively, with no significant differences between the groups. A significant negative correlation existed between Qc eff and haematocrit in the preterm infants. There was no relationship between weight standardized Qc eff and postnatal age in any of the groups. With this technique, it was possible to readily recognise the presence of rapid recirculation (indicative of shunting) in several of the infants, suggesting that rebreathing methods for the assessment of Qc eff should not be applied indiscriminately during the neonatal period. By taking care to overcome the potential sources of technical error, it was possible to obtain highly reproducible results of Qc eff in infants over a wider age range than has been previously reported. PMID:838861

Non Destructive Analysis of Fsw Welds using Ultrasonic Signal Analysis

NASA Astrophysics Data System (ADS)

Pavan Kumar, T.; Prabhakar Reddy, P.

2017-08-01

Friction Stir Welding is an evolving metal joining technique and is mostly used in joining materials which cannot be easily joined by other available welding techniques. It is a technique which can be used for welding dissimilar materials also. The strength of the weld joint is determined by the way in which these material are mixing with each other, since we are not using any filler material for the welding process the intermixing has a significant importance. The complication with the friction stir welding process is that there are many process parameters which effect this intermixing process such as tool geometry, rotating speed of the tool, transverse speed etc., In this study an attempt is made to compare the material flow and weld quality of various weldments by changing the parameters. Ultrasonic signal Analysis is used to characterize the microstructure of the weldments. use of ultrasonic waves is a non destructive, accurate and fast way of characterization of microstructure. In this method the relationship between the ultrasonic measured parameters and microstructures are evaluated using background echo and backscattered signal process techniques. The ultrasonic velocity and attenuation measurements are dependent on the elastic modulus and any change in the microstructure is reflected in the ultrasonic velocity. An insight into material flow is essential to determine the quality of the weld. Hence an attempt is made in this study to know the relationship between tool geometry and the pattern of material flow and resulting weld quality the experiments are conducted to weld dissimilar aluminum alloys and the weldments are characterized using and ultra Sonic signal processing. Characterization is also done using Scanning Electron Microscopy. It is observed that there is a good correlation between the ultrasonic signal processing results and Scanning Electron Microscopy on the observed precipitates. Tensile tests and hardness tests are conducted on the

Ultrasonic monitoring of spontaneous imbibition experiments: Acoustic signature of fluid migration

NASA Astrophysics Data System (ADS)

David, Christian; Barnes, Christophe; Desrues, Mathilde; Pimienta, Lucas; Sarout, Joël.; Dautriat, Jérémie

2017-07-01

Capillary rise experiments (spontaneous imbibition tests) were conducted in the laboratory with ultrasonic and X-ray monitoring on the Sherwood sandstone and the Majella grainstone. The aim was to provide a direct comparison between the variation in seismic attributes (amplitude, velocity, spectral content, and energy) and the actual fluid distribution in the rock. Two pairs of ultrasonic P wave sensors located at different heights on a cylindrical rock specimen recorded every 5 s the waveforms when capillary forces make water rise up into the rock from the bottom in contact with a water tank. Simultaneously, computerized tomography scan images of a vertical cross section were also recorded. Two important results were found. (i) The amplitude of the first P wave arrival is impacted by the upward moving fluid front before the P wave velocity is, while the fluid front has not yet reached the sensors level. In contrast, the P wave velocity decreases when the fluid front reaches the Fresnel clearance zone. The spectral analysis of the waveforms shows that the peak frequency amplitude is continuously decreasing without noticeable frequency shift. (ii) A methodology based on the calculation of the analytical signal and instantaneous phase was designed to decompose each waveform into discrete wavelets associated with direct or reflected waves. The energy carried by the wavelets is very sensitive to the fluid substitution process: the coda wavelets related to reflections on the bottom end face of the specimen are impacted as soon as imbibition starts and can be used as a precursor for the arriving fluid.

Study of ultrasonic thermometry based on ultrasonic time-of-flight measurement

NASA Astrophysics Data System (ADS)

Jia, Ruixi; Xiong, Qingyu; Wang, Lijie; Wang, Kai; Shen, Xuehua; Liang, Shan; Shi, Xin

2016-03-01

Ultrasonic thermometry is a kind of acoustic pyrometry and it has been evolving as a new temperature measurement technology for various environment. However, the accurate measurement of the ultrasonic time-of-flight is the key for ultrasonic thermometry. In this paper, we study the ultrasonic thermometry technique based on ultrasonic time-of-flight measurement with a pair of ultrasonic transducers for transmitting and receiving signal. The ultrasonic transducers are installed in a single path which ultrasonic travels. In order to validate the performance of ultrasonic thermometry, we make a contrast about the absolute error between the measured temperature value and the practical one. With and without heater source, the experimental results indicate ultrasonic thermometry has high precision of temperature measurement.

Do Anesthetic Techniques Influence the Threshold for Glomerular Capillary Hemorrhage Induced in Rats by Contrast-Enhanced Diagnostic Ultrasound?

PubMed

Miller, Douglas L; Lu, Xiaofang; Fabiilli, Mario; Dou, Chunyan

2016-02-01

Glomerular capillary hemorrhage can be induced by ultrasonic cavitation during contrast-enhanced diagnostic ultrasound (US) exposure, an important nonthermal US bioeffect. Recent studies of pulmonary US exposure have shown that thresholds for another nonthermal bioeffect of US, pulmonary capillary hemorrhage, is strongly influenced by whether xylazine is included in the specific anesthetic technique. The objective of this study was to determine the influence of xylazine on contrast-enhanced diagnostic US-induced glomerular capillary hemorrhage. In this study, anesthesia with ketamine only was compared to ketamine plus xylazine for induction of glomerular capillary hemorrhage in rats by 1.6-MHz intermittent diagnostic US with a microsphere contrast agent (similar to Definity; Lantheus Medical Imaging, Inc, North Billerica, MA). Glomerular capillary hemorrhage was measured as a percentage of glomeruli with hemorrhage found in histologic sections for groups of rats scanned at different peak rarefactional pressure amplitudes. There was a significant difference between the magnitude of the glomerular capillary hemorrhage between the anesthetics at 2.3 MPa, with 45.6% hemorrhage for ketamine only, increasing to 63.2% hemorrhage for ketamine plus xylazine (P < .001). However, the thresholds for the two anesthetic methods were virtually identical at 1.0 MPa, based on linear regression of the exposure response data. Thresholds for contrast-enhanced diagnostic US-induced injury of the microvasculature appear to be minimally affected by anesthetic methods. © 2016 by the American Institute of Ultrasound in Medicine.

A novel green analytical procedure for monitoring of azoxystrobin in water samples by a flow injection chemiluminescence method with off-line ultrasonic treatment.

PubMed

Yang, Xin-an; Zhang, Wang-bing

2013-01-01

A simple and green flow injection chemiluminescence (FI-CL) method for determination of the fungicide azoxystrobin was described for the first time. CL signal was generated when azoxystrobin was injected into a mixed stream of luminol and KMnO4 . The CL signal of azoxystrobin could be greatly improved when an off-line ultrasonic treatment was adopted. Meanwhile, the signal intensity increases with the analyte concentration proportionally. Several variables, such as the ultrasonic parameters, flow rate of reagents, concentrations of sodium hydroxide solution and CL reagents (potassium permanganate, luminol) were investigated, and the optimal CL conditions were obtained. Under optimal conditions, the linear range of 1-100 ng/mL for azoxystrobin was obtained and the detection limit (3σ) was determined as 0.13 ng/mL. The relative standard deviation was 1.5% for 10 consecutive measurements of 20 ng/mL azoxystrobin. The method has been applied to the determination of azoxystrobin residues in water samples. Copyright © 2012 John Wiley & Sons, Ltd.

Functionalized core-shell hydrogel microsprings by anisotropic gelation with bevel-tip capillary

PubMed Central

Yoshida, Koki; Onoe, Hiroaki

2017-01-01

This study describes a novel microfluidic-based method for the synthesis of hydrogel microsprings that are capable of encapsulating various functional materials. A continuous flow of alginate pre-gel solution can spontaneously form a hydrogel microspring by anisotropic gelation around the bevel-tip of the capillary. This technique allows fabrication of hydrogel microsprings using only simple capillaries and syringe pumps, while their complex compartmentalization characterized by a laminar flow inside the capillary can contribute to the optimization of the microspring internal structure and functionality. Encapsulation of several functional materials including magnetic-responsive nanoparticles or cell dispersed collagen for tissue scaffold was demonstrated to functionalize the microsprings. Our core-shell hydrogel microsprings have immense potential for application in a number of fields, including biological/chemical microsensors, biocompatible soft robots/microactuators, drug release, self-assembly of 3D structures and tissue engineering. PMID:28378803

Two-dimensional capillary electrophoresis: capillary isoelectric focusing and capillary zone electrophoresis with laser-induced fluorescence detection

PubMed Central

Dickerson, Jane A.; Ramsay, Lauren M.; Dada, Oluwatosin O.; Cermak, Nathan

2011-01-01

Capillary isoelectric focusing and capillary zone electrophoresis are coupled with laser-induced fluorescence detection to create an ultrasensitive two-dimensional separation method for proteins. In this method, two capillaries are joined through a buffer filled interface. Separate power supplies control the potential at the injection end of the first capillary and at the interface; the detector is held at ground potential. Proteins are labeled with the fluorogenic reagent Chromeo P503, which preserves the isoelectric point of the labeled protein. The labeled proteins were mixed with ampholytes and injected into the first dimension capillary. A focusing step was performed with the injection end of the capillary at high pH and the interface at low pH. To mobilize components, the interface was filled with a high pH buffer, which was compatible with the second dimension separation. A fraction was transferred to the second dimension capillary for separation. The process of fraction transfer and second dimension separation was repeated two dozen times. The separation produced a spot capacity of 125. PMID:20603830

Control of electroosmosis in coated quartz capillaries

NASA Technical Reports Server (NTRS)

Herren, Blair J.; Van Alstine, James; Snyder, Robert S.; Shafer, Steven G.; Harris, J. Milton

1987-01-01

The effectiveness of various coatings for controlling the electroosmotic fluid flow that hinders electrophoretic processes is studied using analytical particle microelectrophoresis. The mobilities of 2-micron diameter glass and polystyrene latex spheres (exhibiting both negative and zero effective surface charge) were measured in 2-mm diameter quartz capillaries filled with NaCl solutions within the 3.5-7.8 pH range. It is found that capillary inner surface coatings using 5000 molecular weight (or higher) poly(ethylene glycol): significantly reduced electroosmosis within the selected pH range, were stable for long time periods, and appeared to be more effective than dextran, methylcellulose, or silane coatings.

Development and evaluation of an ultrasonic ground water seepage meter.

PubMed

Paulsen, R J; Smith, C F; O'Rourke, D; Wong, T F

2001-01-01

Submarine ground water discharge can influence significantly the near-shore transport and flux of chemicals into the oceans. Quantification of the sources and rates of such discharge requires a ground water seepage meter that provides continuous measurements at high resolution over an extended period of time. An ultrasonic flowmeter has been adapted for such measurements in the submarine environment. Connected to a steel collection funnel, the meter houses two piezoelectric transducers mounted at opposite ends of a cylindrical flow tube. By monitoring the perturbations of fluid flow on the propagation of sound waves inside the flow tube, the ultrasonic meter can measure both forward and reverse fluid flows in real time. Laboratory and field calibrations show that the ultrasonic meter can resolve ground water discharges on the order of 0.1 microm/sec, and it is sufficiently robust for deployment in the field for several days. Data from West Neck Bay, Shelter Island, New York, elucidate the temporal and spatial heterogeneity of submarine ground water discharge and its interplay with tidal loading. A negative correlation between the discharge and tidal elevation was generally observed. A methodology was also developed whereby data for the sound velocity as a function of temperature can be used to infer the salinity and source of the submarine discharge. Independent measurements of electrical conductance were performed to validate this methodology.

Highly sensitive oligosaccharide analysis in capillary electrophoresis using large-volume sample stacking with an electroosmotic flow pump.

PubMed

Kawai, Takayuki; Watanabe, Masato; Sueyoshi, Kenji; Kitagawa, Fumihiko; Otsuka, Koji

2012-04-06

To obtain high sensitivity in capillary electrophoresis of oligosaccharide without reducing the high resolution with an easy experimental procedure, large-volume sample stacking with an electroosmotic flow pump (LVSEP) was investigated. As a fundamental study, effect of the conductivity of a sample solution in LVSEP was examined. It was revealed that LVSEP was successfully carried out even in using a sample solution with the ionic strength of 150 μM and the conductivity ratio of 20, indicating a good applicability of LVSEP to the analysis of real samples containing salts. When glucose oligomer was analyzed as a model sample in LVSEP-capillary zone electrophoresis (CZE), all peaks were well resolved with decreasing only 5% of the peak-to-peak distance, which suggested 95% of the whole capillary could be used for the effective separation. In the analysis of maltoheptaose, a good calibration line with correlation coefficient of 0.9995 was obtained. The limit of detection was estimated as 2 pM, which was 500-fold lower than that in the conventional CZE. N-linked glycans released from three glycoproteins, bovine ribonuclease B, bovine fetuin, and human α(1)-acid glycoprotein were also analyzed by LVSEP-CZE. By the sample purification with a gel filtration column, further sample dilution to reduce the sample conductivity for LVSEP was not needed. All glycan samples were well concentrated and separated with up to a 770-fold sensitivity increase. The run-to-run repeatabilities of the migration time, peak height, and peak area were good with relative standard deviations of 0.1-1.3%, 1.2-1.7%, and 2.8-4.9%, respectively. Copyright © 2011 Elsevier B.V. All rights reserved.

Ultrasonic pretreatment for enhanced saccharification and fermentation of ethanol production from corn

NASA Astrophysics Data System (ADS)

Montalbo-Lomboy, Melissa T.

during pretreatment. Ultrasonication of sugary-2 corn was also investigated in the study. Results similar to those for commodity corn (dent corn) were found, in terms of glucose yield and starch conversion. SEM and polarized-light microscope pictures showed the partial gelatinization of corn slurry due to ultrasound. In the 96-h saccharification time, a model was formulated to fit the sugar release curve. The results have shown 17-21% increase in the extent of sugar production from sonicated samples relative to the control group. Additionally, the reaction rates of the sonicated samples were 2- to 10-fold higher than the reaction rates for the control group. In comparing sugary-2 corn with commodity corn, it was found that sonicated sugary-2 corn saccharified faster than sonicated commodity corn. It is important to note, without ultrasonic treatment, sugary-2 corn released more reducing sugar than commodity corn during saccharification. To further investigate the potential of ultrasonics for scale-up, a continuous flow system was studied. An ultrasonic continuous flow system was tested using Branson's flow-through "donut" horn. The donut horn, which vibrates radially, was placed inside a 5.5 L stainless steel reactor. The amplitude was maintained at 12 mumpp and the feed flow rate was varied from 8-27 L/min (2-7 gal/min) with reactor retention times varying from 12-40 seconds. Samples sonicated in continuous flow system showed lower reducing sugar yield than batch ultrasonication. However, considering the ultrasonic energy density of batch and continuous systems, the continuous systems proved to be more energy efficient in terms of glucose production compared with the batch system. It was also seen that particle size disintegration was proportional to energy density regardless of the type of ultrasonic system used. To compare ultrasonics with jet cooking, fermentation experiments were conducted. There were only marginal differences between jet cooked samples and the

Ultrasonic Waves in Water Visualized With Schlieren Imaging

NASA Technical Reports Server (NTRS)

Juergens, Jeffrey R.

2000-01-01

The Acoustic Liquid Manipulation project at the NASA Glenn Research Center at Lewis Field is working with high-intensity ultrasound waves to produce acoustic radiation pressure and acoustic streaming. These effects can be used to propel liquid flows to manipulate floating objects and liquid surfaces. Interest in acoustic liquid manipulation has been shown in acoustically enhanced circuit board electroplating, microelectromechanical systems (MEMS), and microgravity space experiments. The current areas of work on this project include phased-array ultrasonic beam steering, acoustic intensity measurements, and schlieren imaging of the ultrasonic waves.

Surfactant-induced electroosmotic flow in microfluidic capillaries.

PubMed

Azadi, Glareh; Tripathi, Anubhav

2012-07-01

Control of EOF in microfluidic devices is essential in applications such as protein/DNA sizing and high-throughput drug screening. With the growing popularity of poly(methyl methacrylate) (PMMA) as the substrate for polymeric-based microfludics, it is important to understand the effect of surfactants on EOF in these devices. In this article, we present an extensive investigation exploring changes in EOF rate induced by SDS, polyoxyethylene lauryl ether (Brij35) and CTAB in PMMA microfluidic capillaries. In a standard protein buffer (Tris-Glycine), PMMA capillaries exhibited a cathodic EOF with measured mobility of 1.54 ± 0.1 (× 10⁻⁴ cm²/V.s). In the presence of surfactant below a critical concentration, EOF was independent of surfactant concentration. At high concentrations of surfactants, the electroosmotic mobility was found to linearly increase/decrease as the logarithm of concentration before reaching a constant value. With SDS, the EOF increased by 257% (compared to buffer), while it was decreased by 238% with CTAB. In the case of Brij35, the electroosmotic mobility was reduced by 70%. In a binary surfactant system of SDS/CTAB and SDS/Brij35, addition of oppositely charged CTAB reduced the SDS-induced EOF more effectively compared to nonionic Brij35. We propose possible mechanisms that explain the observed changes in EOF and zeta potential values. Use of neutral polymer coatings in combination with SDS resulted in 50% reduction in the electroosmotic mobility with 0.1% hydroxypropyl methyl cellulose (HPMC), while including 2% poly (N,N-dimethylacrylamide) (PDMA) had no effect. These results will potentially contribute to the development of PMMA-based microfluidic devices. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Ultrasonic pulser-receiver

DOEpatents

Taylor, Steven C.

2006-09-12

Ultrasonic pulser-receiver circuitry, for use with an ultrasonic transducer, the circuitry comprising a circuit board; ultrasonic pulser circuitry supported by the circuit board and configured to be coupled to an ultrasonic transducer and to cause the ultrasonic transducer to emit an ultrasonic output pulse; receiver circuitry supported by the circuit board, coupled to the pulser circuitry, including protection circuitry configured to protect against the ultrasonic pulse and including amplifier circuitry configured to amplify an echo, received back by the transducer, of the output pulse; and a connector configured to couple the ultrasonic transducer directly to the circuit board, to the pulser circuitry and receiver circuitry, wherein impedance mismatches that would result if the transducer was coupled to the circuit board via a cable can be avoided.

Triggering vacuum capillaries for pneumatic pumping and metering liquids in point-of-care immunoassays.

PubMed

Weng, Kuo-Yao; Chou, Nien-Jen; Cheng, Jya-Wei

2008-07-01

An innovative vacuum capillary pneumatic actuation concept that can be used for point-of-care testing has been investigated. The vacuum glass capillaries are encapsulated within a laminated pouch and incorporated into the fluidic card. Vacuum glass capillaries broken by external force such as finger pressure, generate the pneumatic forces to induce liquid flow in the fluidic system. The sizes of vacuum capillary play a vital role in the pumping and metering functions of the system. The luteinizing hormone (LH) chromatographic immunoassay performances in the fluidic cards show consistency comparable to that obtained by manual micropipetting. The vacuum capillary pneumatic actuation will be applied in other complex handling step bioassays and lab-on-a-chip devices.

A new contrast-assisted method in microcirculation volumetric flow assessment

NASA Astrophysics Data System (ADS)

Lu, Sheng-Yi; Chen, Yung-Sheng; Yeh, Chih-Kuang

2007-03-01

Microcirculation volumetric flow rate is a significant index in diseases diagnosis and treatment such as diabetes and cancer. In this study, we propose an integrated algorithm to assess microcirculation volumetric flow rate including estimation of blood perfused area and corresponding flow velocity maps based on high frequency destruction/contrast replenishment imaging technique. The perfused area indicates the blood flow regions including capillaries, arterioles and venules. Due to the echo variance changes between ultrasonic contrast agents (UCAs) pre- and post-destruction two images, the perfused area can be estimated by the correlation-based approach. The flow velocity distribution within the perfused area can be estimated by refilling time-intensity curves (TICs) after UCAs destruction. Most studies introduced the rising exponential model proposed by Wei (1998) to fit the TICs. Nevertheless, we found the TICs profile has a great resemblance to sigmoid function in simulations and in vitro experiments results. Good fitting correlation reveals that sigmoid model was more close to actual fact in describing destruction/contrast replenishment phenomenon. We derived that the saddle point of sigmoid model is proportional to blood flow velocity. A strong linear relationship (R = 0.97) between the actual flow velocities (0.4-2.1 mm/s) and the estimated saddle constants was found in M-mode and B-mode flow phantom experiments. Potential applications of this technique include high-resolution volumetric flow rate assessment in small animal tumor and the evaluation of superficial vasculature in clinical studies.

Capillary descent.

PubMed

Delannoy, Joachim; de Maleprade, Hélène; Clanet, Christophe; Quéré, David

2018-05-31

A superhydrophobic capillary tube immersed in water and brought in contact with the bath surface will be invaded by air, owing to its aerophilicity. We discuss this phenomenon where the ingredients of classical capillary rise are inverted, which leads to noticeable dynamical features. (1) The main regime of air invasion is linear in time, due to the viscous resistance of water. (2) Menisci in tubes with millimetre-size radii strongly oscillate before reaching their equilibrium depth, a consequence of inertia. On the whole, capillary descent provides a broad variety of dynamics where capillary effects, viscous friction and liquid inertia all play a role.

Development of novel separation techniques for biological samples in capillary electrophoresis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chang, Huan -Tsung

1994-07-27

This dissertation includes three different topics: general introduction of capillary electrophoresis (CE); gradient in CE and CE in biological separations; and capillary gel electrophoresis (CGE) for DNA separation. Factors such as temperature, viscosity, pH, and the surface of capillary walls affecting the separation performance are demonstrated. A pH gradient between 3.0 and 5.2 is useful to improve the resolution among eight different organic acids. A flow gradient due to the change in the concentration of surfactant, which is able to coat to the capillary wall to change the flow rate and its direction, is also shown as a good waymore » to improve the resolution for organic compounds. A temperature gradient caused by joule heat is shown by voltage programming to enhance the resolution and shorten the separation time for several phenolic compounds. The author also shows that self-regulating dynamic control of electroosmotic flow in CE by simply running separation in different concentrations of surfactant has less matrix effect on the separation performance. One of the most important demonstrations in this dissertation is that the author proposes on-column reaction which gives several advantages including the use of a small amount of sample, low risk of contamination, and time saving and kinetic features. The author uses this idea with laser induced fluorescence (LIF) as a detection mode to detect an on-column digestion of sub-ng of protein. This technique also is applied to single cell analysis in the group.« less

Grain fragmentation in ultrasonic-assisted TIG weld of pure aluminum.

PubMed

Chen, Qihao; Lin, Sanbao; Yang, Chunli; Fan, Chenglei; Ge, Hongliang

2017-11-01

Under the action of acoustic waves during an ultrasonic-assisted tungsten inert gas (TIG) welding process, a grain of a TIG weld of aluminum alloy is refined by nucleation and grain fragmentation. Herein, effects of ultrasound on grain fragmentation in the TIG weld of aluminum alloy are investigated via systematic welding experiments of pure aluminum. First, experiments involving continuous and fixed-position welding are performed, which demonstrate that ultrasound can break the grain of the TIG weld of pure aluminum. The microstructural characteristics of an ultrasonic-assisted TIG weld fabricated by fixed-position welding are analyzed. The microstructure is found to transform from plane crystal, columnar crystal, and uniform equiaxed crystal into plane crystal, deformed columnar crystal, and nonuniform equiaxed crystal after application of ultrasound. Second, factors influencing ultrasonic grain fragmentation are investigated. The ultrasonic amplitude and welding current are found to have a considerable effect on grain fragmentation. The degree of fragmentation first increases and then decreases with an increase in ultrasonic amplitude, and it increases with an increase in welding current. Measurement results of the vibration of the weld pool show that the degree of grain fragmentation is related to the intensity of acoustic nonlinearity in the weld pool. The greater the intensity of acoustic nonlinearity, the greater is the degree of grain fragmentation. Finally, the mechanism of ultrasonic grain fragmentation in the TIG weld of pure aluminum is discussed. A finite element simulation is used to simulate the acoustic pressure and flow in the weld pool. The acoustic pressure in the weld pool exceeds the cavitation threshold, and cavitation bubbles are generated. The flow velocity in the weld pool does not change noticeably after application of ultrasound. It is concluded that the high-pressure conditions induced during the occurrence of cavitation, lead to grain

A novel instrument for studying the flow behaviour of erythrocytes through microchannels simulating human blood capillaries.

PubMed

Sutton, N; Tracey, M C; Johnston, I D; Greenaway, R S; Rampling, M W

1997-05-01

A novel instrument has been developed to study the microrheology of erythrocytes as they flow through channels of dimensions similar to human blood capillaries. The channels are produced in silicon substrates using microengineering technology. Accurately defined, physiological driving pressures and temperatures are employed whilst precise, real-time image processing allows individual cells to be monitored continuously during their transit. The instrument characterises each cell in a sample of ca. 1000 in terms of its volume and flow velocity profile during its transit through a channel. The unique representation of the data in volume/velocity space provides new insight into the microrheological behaviour of blood. The image processing and subsequent data analysis enable the system to reject anomalous events such as multiple cell transits, thereby ensuring integrity of the resulting data. By employing an array of microfluidic flow channels we can integrate a number of different but precise and highly reproducible channel sizes and geometries within one array, thereby allowing multiple, concurrent isobaric measurements on one sample. As an illustration of the performance of the system, volume/velocity data sets recorded in a microfluidic device incorporating multiple channels of 100 microns length and individual widths ranging between 3.0 and 4.0 microns are presented.

The role of the cerebral capillaries in acute ischemic stroke: the extended penumbra model.

PubMed

Østergaard, Leif; Jespersen, Sune Nørhøj; Mouridsen, Kim; Mikkelsen, Irene Klærke; Jonsdottír, Kristjana Ýr; Tietze, Anna; Blicher, Jakob Udby; Aamand, Rasmus; Hjort, Niels; Iversen, Nina Kerting; Cai, Changsi; Hougaard, Kristina Dupont; Simonsen, Claus Z; Von Weitzel-Mudersbach, Paul; Modrau, Boris; Nagenthiraja, Kartheeban; Riisgaard Ribe, Lars; Hansen, Mikkel Bo; Bekke, Susanne Lise; Dahlman, Martin Gervais; Puig, Josep; Pedraza, Salvador; Serena, Joaquín; Cho, Tae-Hee; Siemonsen, Susanne; Thomalla, Götz; Fiehler, Jens; Nighoghossian, Norbert; Andersen, Grethe

2013-05-01

The pathophysiology of cerebral ischemia is traditionally understood in relation to reductions in cerebral blood flow (CBF). However, a recent reanalysis of the flow-diffusion equation shows that increased capillary transit time heterogeneity (CTTH) can reduce the oxygen extraction efficacy in brain tissue for a given CBF. Changes in capillary morphology are typical of conditions predisposing to stroke and of experimental ischemia. Changes in capillary flow patterns have been observed by direct microscopy in animal models of ischemia and by indirect methods in humans stroke, but their metabolic significance remain unclear. We modeled the effects of progressive increases in CTTH on the way in which brain tissue can secure sufficient oxygen to meet its metabolic needs. Our analysis predicts that as CTTH increases, CBF responses to functional activation and to vasodilators must be suppressed to maintain sufficient tissue oxygenation. Reductions in CBF, increases in CTTH, and combinations thereof can seemingly trigger a critical lack of oxygen in brain tissue, and the restoration of capillary perfusion patterns therefore appears to be crucial for the restoration of the tissue oxygenation after ischemic episodes. In this review, we discuss the possible implications of these findings for the prevention, diagnosis, and treatment of acute stroke.

The role of the cerebral capillaries in acute ischemic stroke: the extended penumbra model

PubMed Central

Østergaard, Leif; Jespersen, Sune Nørhøj; Mouridsen, Kim; Mikkelsen, Irene Klærke; Jonsdottír, Kristjana Ýr; Tietze, Anna; Blicher, Jakob Udby; Aamand, Rasmus; Hjort, Niels; Iversen, Nina Kerting; Cai, Changsi; Hougaard, Kristina Dupont; Simonsen, Claus Z; Von Weitzel-Mudersbach, Paul; Modrau, Boris; Nagenthiraja, Kartheeban; Riisgaard Ribe, Lars; Hansen, Mikkel Bo; Bekke, Susanne Lise; Dahlman, Martin Gervais; Puig, Josep; Pedraza, Salvador; Serena, Joaquín; Cho, Tae-Hee; Siemonsen, Susanne; Thomalla, Götz; Fiehler, Jens; Nighoghossian, Norbert; Andersen, Grethe

2013-01-01

The pathophysiology of cerebral ischemia is traditionally understood in relation to reductions in cerebral blood flow (CBF). However, a recent reanalysis of the flow-diffusion equation shows that increased capillary transit time heterogeneity (CTTH) can reduce the oxygen extraction efficacy in brain tissue for a given CBF. Changes in capillary morphology are typical of conditions predisposing to stroke and of experimental ischemia. Changes in capillary flow patterns have been observed by direct microscopy in animal models of ischemia and by indirect methods in humans stroke, but their metabolic significance remain unclear. We modeled the effects of progressive increases in CTTH on the way in which brain tissue can secure sufficient oxygen to meet its metabolic needs. Our analysis predicts that as CTTH increases, CBF responses to functional activation and to vasodilators must be suppressed to maintain sufficient tissue oxygenation. Reductions in CBF, increases in CTTH, and combinations thereof can seemingly trigger a critical lack of oxygen in brain tissue, and the restoration of capillary perfusion patterns therefore appears to be crucial for the restoration of the tissue oxygenation after ischemic episodes. In this review, we discuss the possible implications of these findings for the prevention, diagnosis, and treatment of acute stroke. PMID:23443173

Split and flow: reconfigurable capillary connection for digital microfluidic devices.

PubMed

Lapierre, Florian; Harnois, Maxime; Coffinier, Yannick; Boukherroub, Rabah; Thomy, Vincent

2014-09-21

Supplying liquid to droplet-based microfluidic microsystems remains a delicate task facing the problems of coupling continuous to digital or macro- to microfluidic systems. Here, we take advantage of superhydrophobic microgrids to address this problem. Insertion of a capillary tube inside a microgrid aperture leads to a simple and reconfigurable droplet generation setup.

Development of an Ultrasonic Resonator for Ballast Water Disinfection

NASA Astrophysics Data System (ADS)

Osman, Hafiiz; Lim, Fannon; Lucas, Margaret; Balasubramaniam, Prakash

Ultrasonic disinfection involves the application of low-frequency acoustic energy in a water body to induce cavitation. The implosion of cavitation bubbles generates high speed microjets >1 km/s, intense shock wave >1 GPa, localized hot spots >1000 K, and free-radicals, resulting in cell rupture and death of micro-organisms and pathogens. Treatment of marine ballast water using power ultrasonics is an energy-intensive process. Compared with other physical treatment methods such as ultraviolet disinfection, ultrasonic disinfection require 2 to 3 orders of magnitude more energy to achieve similar rate of micro-organism mortality. Current technology limits the amount of acoustic energy that can be transferred per unit volume of fluid and presents challenges when it comes to high-flow applications. Significant advancements in ultrasonic processing technology are needed before ultrasound can be recognized as a viable alternative disinfection method. The ultrasonic resonator has been identified as one of the areas of improvement that can potentially contribute to the overall performance of an ultrasonic disinfection system. The present study focuses on the design of multiple-orifice resonators (MOR) for generating a well-distributed cavitation field. Results show that the MOR resonator offers significantly larger vibrational surface area to mass ratio. In addition, acoustic pressure measurements indicate that the MOR resonators are able to distribute the acoustic energy across a larger surface area, while generating 2-4 times higher pressures than existing ultrasonic probes.

New Analysis Scheme of Flow-Acoustic Coupling for Gas Ultrasonic Flowmeter with Vortex near the Transducer

PubMed Central

Zhang, Tao; Zheng, Dandan

2018-01-01

Ultrasonic flowmeters with a small or medium diameter are widely used in process industries. The flow field disturbance on acoustic propagation caused by a vortex near the transducer inside the sensor as well as the mechanism and details of flow-acoustic interaction are needed to strengthen research. For that reason, a new hybrid scheme is proposed; the theories of computational fluid dynamics (CFD), wave acoustics, and ray acoustics are used comprehensively by a new step-by-step method. The flow field with a vortex near the transducer, and its influence on sound propagation, receiving, and flowmeter performance are analyzed in depth. It was found that, firstly, the velocity and vortex intensity distribution were asymmetric on the sensor cross-section and acoustic path. Secondly, when passing through the vortex zone, the central ray trajectory was deflected significantly. The sound pressure on the central line of the sound path also changed. Thirdly, the pressure deviation becomes larger with as the flow velocity increases. The deviation was up to 17% for different velocity profiles in a range of 0.6 m/s to 53 m/s. Lastly, in comparison to the theoretical value, the relative deviation of the instrument coefficient for the velocity profile with a vortex near the transducer reached up to −17%. In addition, the rationality of the simulation was proved by experiments. PMID:29642577

New Analysis Scheme of Flow-Acoustic Coupling for Gas Ultrasonic Flowmeter with Vortex near the Transducer.

PubMed

Sun, Yanzhao; Zhang, Tao; Zheng, Dandan

2018-04-10

Ultrasonic flowmeters with a small or medium diameter are widely used in process industries. The flow field disturbance on acoustic propagation caused by a vortex near the transducer inside the sensor as well as the mechanism and details of flow-acoustic interaction are needed to strengthen research. For that reason, a new hybrid scheme is proposed; the theories of computational fluid dynamics (CFD), wave acoustics, and ray acoustics are used comprehensively by a new step-by-step method. The flow field with a vortex near the transducer, and its influence on sound propagation, receiving, and flowmeter performance are analyzed in depth. It was found that, firstly, the velocity and vortex intensity distribution were asymmetric on the sensor cross-section and acoustic path. Secondly, when passing through the vortex zone, the central ray trajectory was deflected significantly. The sound pressure on the central line of the sound path also changed. Thirdly, the pressure deviation becomes larger with as the flow velocity increases. The deviation was up to 17% for different velocity profiles in a range of 0.6 m/s to 53 m/s. Lastly, in comparison to the theoretical value, the relative deviation of the instrument coefficient for the velocity profile with a vortex near the transducer reached up to -17%. In addition, the rationality of the simulation was proved by experiments.

Ultrasonic pipe assessment

DOEpatents

Thomas, Graham H.; Morrow, Valerie L.; Levie, Harold; Kane, Ronald J.; Brown, Albert E.

2003-12-23

An ultrasonic pipe or other structure assessment system includes an ultrasonic transducer positioned proximate the pipe or other structure. A fluid connection between the ultrasonic transducer and the pipe or other structure is produced. The ultrasonic transducer is moved relative to the pipe or other structure.

Evans blue dye-enhanced capillary-resolution photoacoustic microscopy in vivo

NASA Astrophysics Data System (ADS)

Yao, Junjie; Maslov, Konstantin; Hu, Song; Wang, Lihong V.

2009-09-01

Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBCs) as an endogenic contrast agent. However, intermittent RBC flow in capillaries results in discontinuous and fragmentary capillary images. To overcome this problem, we use Evans blue (EB) dye as a contrast agent for in vivo photoacoustic imaging. EB has strong optical absorption and distributes uniformly in the blood stream by chemically binding to albumin. With the help of EB, complete and continuous microvascular networks--especially capillaries--are imaged. The diffusion dynamics of EB leaving the blood stream and the clearance dynamics of the EB-albumin complex are also quantitatively investigated.

High-throughput biological small-angle X-ray scattering with a robotically loaded capillary cell

PubMed Central

Nielsen, S. S.; Møller, M.; Gillilan, R. E.

2012-01-01

With the rise in popularity of biological small-angle X-ray scattering (BioSAXS) measurements, synchrotron beamlines are confronted with an ever-increasing number of samples from a wide range of solution conditions. To meet these demands, an increasing number of beamlines worldwide have begun to provide automated liquid-handling systems for sample loading. This article presents an automated sample-loading system for BioSAXS beamlines, which combines single-channel disposable-tip pipetting with a vacuum-enclosed temperature-controlled capillary flow cell. The design incorporates an easily changeable capillary to reduce the incidence of X-ray window fouling and cross contamination. Both the robot-control and the data-processing systems are written in Python. The data-processing code, RAW, has been enhanced with several new features to form a user-friendly BioSAXS pipeline for the robot. The flow cell also supports efficient manual loading and sample recovery. An effective rinse protocol for the sample cell is developed and tested. Fluid dynamics within the sample capillary reveals a vortex ring pattern of circulation that redistributes radiation-damaged material. Radiation damage is most severe in the boundary layer near the capillary surface. At typical flow speeds, capillaries below 2 mm in diameter are beginning to enter the Stokes (creeping flow) regime in which mixing due to oscillation is limited. Analysis within this regime shows that single-pass exposure and multiple-pass exposure of a sample plug are functionally the same with regard to exposed volume when plug motion reversal is slow. The robot was tested on three different beamlines at the Cornell High-Energy Synchrotron Source, with a variety of detectors and beam characteristics, and it has been used successfully in several published studies as well as in two introductory short courses on basic BioSAXS methods. PMID:22509071

Miniaturized flow injection analysis system

DOEpatents

Folta, James A.

1997-01-01

A chemical analysis technique known as flow injection analysis, wherein small quantities of chemical reagents and sample are intermixed and reacted within a capillary flow system and the reaction products are detected optically, electrochemically, or by other means. A highly miniaturized version of a flow injection analysis system has been fabricated utilizing microfabrication techniques common to the microelectronics industry. The microflow system uses flow capillaries formed by etching microchannels in a silicon or glass wafer followed by bonding to another wafer, commercially available microvalves bonded directly to the microflow channels, and an optical absorption detector cell formed near the capillary outlet, with light being both delivered and collected with fiber optics. The microflow system is designed mainly for analysis of liquids and currently measures 38.times.25.times.3 mm, but can be designed for gas analysis and be substantially smaller in construction.

Activity restriction, impaired capillary function, and the development of insulin resistance in lean primates.

PubMed

Chadderdon, Scott M; Belcik, J Todd; Smith, Elise; Pranger, Lindsay; Kievit, Paul; Grove, Kevin L; Lindner, Jonathan R

2012-09-01

Insulin produces capillary recruitment in skeletal muscle through a nitric oxide (NO)-dependent mechanism. Capillary recruitment is blunted in obese and diabetic subjects and contributes to impaired glucose uptake. This study's objective was to define whether inactivity, in the absence of obesity, leads to impaired capillary recruitment and contributes to insulin resistance (IR). A comprehensive metabolic and vascular assessment was performed on 19 adult male rhesus macaques (Macaca mulatta) after sedation with ketamine and during maintenance anesthesia with isoflurane. Thirteen normal-activity (NA) and six activity-restricted (AR) primates underwent contrast-enhanced ultrasound to determine skeletal muscle capillary blood volume (CBV) during an intravenous glucose tolerance test (IVGTT) and during contractile exercise. NO bioactivity was assessed by flow-mediated vasodilation. Although there were no differences in weight, basal glucose, basal insulin, or truncal fat, AR primates were insulin resistant compared with NA primates during an IVGTT (2,225 ± 734 vs. 5,171 ± 3,431 μg·ml⁻¹·min⁻¹, P < 0.05). Peak CBV was lower in AR compared with NA primates during IVGTT (0.06 ± 0.01 vs. 0.12 ± 0.02 ml/g, P < 0.01) and exercise (0.10 ± 0.02 vs. 0.20 ± 0.02 ml/g, P < 0.01), resulting in a lower peak skeletal muscle blood flow in both circumstances. The insulin-mediated changes in CBV correlated inversely with the degree of IR and directly with activity. Flow-mediated dilation was lower in the AR primates (4.6 ± 1.0 vs. 9.8 ± 2.3%, P = 0.01). Thus, activity restriction produces impaired skeletal muscle capillary recruitment during a carbohydrate challenge and contributes to IR in the absence of obesity. Reduced NO bioactivity may be a pathological link between inactivity and impaired capillary function.

Speciation of mercury by hydrostatically modified electroosmotic flow capillary electrophoresis coupled with volatile species generation atomic fluorescence spectrometry.

PubMed

Yan, Xiu-Ping; Yin, Xue-Bo; Jiang, Dong-Qing; He, Xi-Wen

2003-04-01

A novel method for speciation analysis of mercury was developed by on-line hyphenating capillary electrophoresis (CE) with atomic fluorescence spectrometry (AFS). The four mercury species of inorganic mercury Hg(II), methymercury MeHg(I), ethylmercury EtHg(I), and phenylmercury PhHg(I) were separated as mercury-cysteine complexes by CE in a 50-cm x 100-microm-i.d. fused-silica capillary at 15 kV and using a mixture of 100 mmol L(-1) of boric acid and 12% v/v methanol (pH 9.1) as electrolyte. A novel technique, hydrostatically modified electroosmotic flow (HSMEOF) in which the electroosmotic flow (EOF) was modified by applying hydrostatical pressure opposite to the direction of EOF was used to improve resolution. A volatile species generation technique was used to convert the mercury species into their respective volatile species. A newly developed CE-AFS interface was employed to provide an electrical connection for stable electrophoretic separations and to allow on-line volatile species formation. The generated volatile species were on-line detected with AFS. The precisions (RSD, n = 5) were in the range of 1.9-2.5% for migration time, 1.8-6.3% for peak area response, and 2.3-6.1% for peak height response for the four mercury species. The detection limits ranged from 6.8 to 16.5 microg L(-1) (as Hg). The recoveries of the four mercury species in the water samples were in the range of 86.6-111%. The developed technique was successfully applied to speciation analysis of mercury in a certified reference material (DORM-2, dogfish muscle).

Piezoelectric single crystals for ultrasonic transducers in biomedical applications

PubMed Central

Zhou, Qifa; Lam, Kwok Ho; Zheng, Hairong; Qiu, Weibao; Shung, K. Kirk

2014-01-01

Piezoelectric single crystals, which have excellent piezoelectric properties, have extensively been employed for various sensors and actuators applications. In this paper, the state–of–art in piezoelectric single crystals for ultrasonic transducer applications is reviewed. Firstly, the basic principles and design considerations of piezoelectric ultrasonic transducers will be addressed. Then, the popular piezoelectric single crystals used for ultrasonic transducer applications, including LiNbO3 (LN), PMN–PT and PIN–PMN–PT, will be introduced. After describing the preparation and performance of the single crystals, the recent development of both the single–element and array transducers fabricated using the single crystals will be presented. Finally, various biomedical applications including eye imaging, intravascular imaging, blood flow measurement, photoacoustic imaging, and microbeam applications of the single crystal transducers will be discussed. PMID:25386032

Capillary pressure spectrometry: Toward a new method for the measurement of the fractional wettability of porous media

NASA Astrophysics Data System (ADS)

Sygouni, Varvara; Tsakiroglou, Christos D.; Payatakes, Alkiviades C.

2006-05-01

A transparent porous medium of controlled fractional wettability is fabricated by mixing intermediate-wet glass microspheres with strongly oil-wet polytetrafluouroethylene microspheres, and packing them between two transparent glass plates. Silicon oil is displaced by water, the growth pattern is video-recorded, and the transient response of the pressure drop across the pore network is measured for various fractions of oil-wet particles. The measured global capillary pressure fluctuates as the result of the variation of the equilibrium curvature of menisci between local maxima and local minima. With the aid of wavelets, the transient response of the capillary pressure is transformed to a capillary pressure spectrum (CPS). The peaks of the CPS are used to identify the most significant flow events and correlate their amplitude with the spatial distribution of fractional wettability. The flow events are closely related with the fluctuations of the capillary pressure and are classified into three main categories: motion in pore clusters, generation/expansion of capillary fingers, coalescence of interfaces. The amplitude of the peaks of CPS is related quasilinearly with a local coefficient of fractional wettability presuming that the same class of flow events is concerned. Approximate calculations of the maximum meniscus curvature in pores of converging-diverging geometry and uniform wettability in combination with simple mixing laws predict satisfactorily the experimentally measured average prebreakthrough capillary pressure as a function of the fraction of the oil-wet particles.

Evaluation of a commercial electro-kinetically pumped sheath-flow nanospray interface coupled to an automated capillary zone electrophoresis system.

PubMed

Peuchen, Elizabeth H; Zhu, Guije; Sun, Liangliang; Dovichi, Norman J

2017-03-01

Capillary zone electrophoresis-electrospray ionization-mass spectrometry (CZE-ESI-MS) is attracting renewed attention for proteomic and metabolomic analysis. An important reason for this interest is the maturation and commercialization of interfaces for coupling CZE with ESI-MS. One of these interfaces is an electro-kinetically pumped sheath flow nanospray interface developed by the Dovichi group, in which a very low sheath flow is generated based on electroosmosis within a glass emitter. CMP Scientific has commercialized this interface as the EMASS-II ion source. In this work, we compared the performance of the EMASS-II ion source with our in-house system. The performance of the systems is equivalent. We also coupled the EMASS-II ion source with a PrinCE Next|480 capillary electrophoresis autosampler and an Orbitrap mass spectrometer, and analyzed this system's performance in terms of sensitivity, reproducibility, and separation performance for separation of tryptic digests, intact proteins, and amino acids. The system produced reproducible analysis of BSA digest; the RSDs of peptide intensity and migration time across 24 runs were less than 20 and 6%, respectively. The system produced a linear calibration curve of intensity across a 30-fold range of tryptic digest concentration. The combination of a commercial autosampler and electrospray interface efficiently separated amino acids, peptides, and intact proteins, and only required 5 μL of sample for analysis. Graphical Abstract The commercial and locally constructed versions of the interface provide similar numbers of protein identifications from a Xenopus laevis fertilized egg digest.

Phosphodiesterase-4 inhibition as a therapeutic approach to treat capillary leakage in systemic inflammation.

PubMed

Schick, Martin Alexander; Wunder, Christian; Wollborn, Jakob; Roewer, Norbert; Waschke, Jens; Germer, Christoph-Thomas; Schlegel, Nicolas

2012-06-01

In sepsis and systemic inflammation, increased microvascular permeability and consecutive breakdown of microcirculatory flow significantly contribute to organ failure and death. Evidence points to a critical role of cAMP levels in endothelial cells to maintain capillary endothelial barrier properties in acute inflammation. However, approaches to verify this observation in systemic models are rare. Therefore we tested here whether systemic application of the phosphodiesterase-4-inhibitors (PD-4-Is) rolipram or roflumilast to increase endothelial cAMP was effective to attenuate capillary leakage and breakdown of microcirculatory flow in severe lipopolysaccharide (LPS)-induced systemic inflammation in rats. Measurements of cAMP in mesenteric microvessels demonstrated significant LPS-induced loss of cAMP levels which was blocked by application of rolipram. Increased endothelial cAMP by application of either PD-4-I rolipram or roflumilast led to stabilization of endothelial barrier properties as revealed by measurements of extravasated FITC-albumin in postcapillary mesenteric venules. Accordingly, microcirculatory flow in mesenteric venules was significantly increased following PD-4-I treatment and blood gas analyses indicated improved metabolism. Furthermore application of PD-4-I after manifestation of LPS-induced systemic inflammation and capillary leakage therapeutically stabilized endothelial barrier properties as revealed by significantly reduced volume resuscitation for haemodynamic stabilization. Accordingly microcirculation was significantly improved following treatment with PD-4-Is. Our results demonstrate that inflammation-derived loss of endothelial cAMP contributes to capillary leakage which was blocked by systemic PD-4-I treatment. Therefore these data suggest a highly clinically relevant and applicable approach to stabilize capillary leakage in sepsis and systemic inflammation.

Ultrasonic Interferometers Revisited

ERIC Educational Resources Information Center

Greenslade, Thomas B., Jr.

2007-01-01

I have been tinkering with ultrasonic transducers once more. In earlier notes I reported on optics-like experiments performed with ultrasonics, described a number of ultrasonic interferometers, and showed how ultrasonic transducers can be used for Fourier analysis. This time I became interested in trying the technique of using two detectors in…

Physiologic upper limits of pore size of different blood capillary types and another perspective on the dual pore theory of microvascular permeability.

PubMed

Sarin, Hemant

2010-08-11

Much of our current understanding of microvascular permeability is based on the findings of classic experimental studies of blood capillary permeability to various-sized lipid-insoluble endogenous and non-endogenous macromolecules. According to the classic small pore theory of microvascular permeability, which was formulated on the basis of the findings of studies on the transcapillary flow rates of various-sized systemically or regionally perfused endogenous macromolecules, transcapillary exchange across the capillary wall takes place through a single population of small pores that are approximately 6 nm in diameter; whereas, according to the dual pore theory of microvascular permeability, which was formulated on the basis of the findings of studies on the accumulation of various-sized systemically or regionally perfused non-endogenous macromolecules in the locoregional tissue lymphatic drainages, transcapillary exchange across the capillary wall also takes place through a separate population of large pores, or capillary leaks, that are between 24 and 60 nm in diameter. The classification of blood capillary types on the basis of differences in the physiologic upper limits of pore size to transvascular flow highlights the differences in the transcapillary exchange routes for the transvascular transport of endogenous and non-endogenous macromolecules across the capillary walls of different blood capillary types. The findings and published data of studies on capillary wall ultrastructure and capillary microvascular permeability to lipid-insoluble endogenous and non-endogenous molecules from the 1950s to date were reviewed. In this study, the blood capillary types in different tissues and organs were classified on the basis of the physiologic upper limits of pore size to the transvascular flow of lipid-insoluble molecules. Blood capillaries were classified as non-sinusoidal or sinusoidal on the basis of capillary wall basement membrane layer continuity or lack thereof

Xenon excimer emission from pulsed high-pressure capillary microdischarges

NASA Astrophysics Data System (ADS)

Lee, Byung-Joon; Rahaman, Hasibur; Petzenhauser, Isfried; Frank, Klaus; Giapis, Konstantinos P.

2007-06-01

Intense xenon vacuum ultraviolet (VUV) emission is observed from a high-pressure capillary cathode microdischarge in direct current operation, by superimposing a high-voltage pulse of 50ns duration. Under stagnant gas conditions, the total VUV light intensity increases linearly with pressure from 400 to 1013mbar for a fixed voltage pulse. At fixed pressure, however, the VUV light intensity increases superlinearly with voltage pulse height ranging from 08to2.8kV. Gains in emission intensity are obtained by inducing gas flow through the capillary cathode, presumably because of excimer dimer survival due to gas cooling.

Custom ultrasonic instrumentation for flow measurement and real-time binary gas analysis in the CERN ATLAS experiment

NASA Astrophysics Data System (ADS)

Alhroob, M.; Battistin, M.; Berry, S.; Bitadze, A.; Bonneau, P.; Boyd, G.; Crespo-Lopez, O.; Degeorge, C.; Deterre, C.; Di Girolamo, B.; Doubek, M.; Favre, G.; Hallewell, G.; Katunin, S.; Lombard, D.; Madsen, A.; McMahon, S.; Nagai, K.; O'Rourke, A.; Pearson, B.; Robinson, D.; Rossi, C.; Rozanov, A.; Stanecka, E.; Strauss, M.; Vacek, V.; Vaglio, R.; Young, J.; Zwalinski, L.

2017-01-01

The development of custom ultrasonic instrumentation was motivated by the need for continuous real-time monitoring of possible leaks and mass flow measurement in the evaporative cooling systems of the ATLAS silicon trackers. The instruments use pairs of ultrasonic transducers transmitting sound bursts and measuring transit times in opposite directions. The gas flow rate is calculated from the difference in transit times, while the sound velocity is deduced from their average. The gas composition is then evaluated by comparison with a molar composition vs. sound velocity database, based on the direct dependence between sound velocity and component molar concentration in a gas mixture at a known temperature and pressure. The instrumentation has been developed in several geometries, with five instruments now integrated and in continuous operation within the ATLAS Detector Control System (DCS) and its finite state machine. One instrument monitors C3F8 coolant leaks into the Pixel detector N2 envelope with a molar resolution better than 2ṡ 10-5, and has indicated a level of 0.14 % when all the cooling loops of the recently re-installed Pixel detector are operational. Another instrument monitors air ingress into the C3F8 condenser of the new C3F8 thermosiphon coolant recirculator, with sub-percent precision. The recent effect of the introduction of a small quantity of N2 volume into the 9.5 m3 total volume of the thermosiphon system was clearly seen with this instrument. Custom microcontroller-based readout has been developed for the instruments, allowing readout into the ATLAS DCS via Modbus TCP/IP on Ethernet. The instrumentation has many potential applications where continuous binary gas composition is required, including in hydrocarbon and anaesthetic gas mixtures.

Numerical analysis of bubble-cluster formation in an ultrasonic field

NASA Astrophysics Data System (ADS)

Kim, Donghyun; Son, Gihun

2016-11-01

Bubble-cluster formation in an ultrasonic field is investigated numerically solving the conservation equations of mass, momentum and energy. The liquid-gas interface is calculated using the volume-of-fluid method with variable gas density to consider the bubble compressibility. The effect of liquid-gas phase change is also included as the interface source terms of the mass and energy equations. The numerical approach is tested through the simulation of the expansion and contraction motion of a compressed bubble adjacent to a wall. When the bubble is placed in an ultrasonic field, it oscillates radially and then collapses violently. Numerical simulation is also performed for bubble-cluster formation induced by an ultrasonic generator, where the generated bubbles are merged into a macrostructure along the acoustic flow field. The effects of ultrasonic power and frequency, liquid properties and pool temperature on the bubble-cluster formation are investigated. This work was supported by the Korea Institute of Energy Research.

Ultrasonic acoustic emissions from the sapwood of cedar and hemlock : an examination of three hypotheses regarding cavitations.

PubMed

Tyree, M T; Dixon, M A; Tyree, E L; Johnson, R

1984-08-01

Measurements are reported of ultrasonic acoustic emissions (AEs) measured from sapwood samples of Thuja occidentalis L. and Tsuga canadensis (L.) Carr. during air dehydration. The measurements were undertaken to test the following three hypotheses: (a) Each cavitation event produces one ultrasonic AE. (b) Large tracheids are more likely to cavitate than small tracheids. (c) When stem water potentials are >-0.4 MPa, a significant fraction of the water content of sapwood is held by ;capillary forces.' The last two hypotheses were recently discussed at length by M. H. Zimmermann. Experimental evidence consistent with all three hypotheses was obtained. The evidence for each hypothesis respectively is: (a) the cumulative number of AEs nearly equals the number of tracheids in small samples; (b) more water is lost per AE event at the beginning of the dehydration process than at the end, and (c) sapwood samples dehydrated from an initial water potential of 0 MPa lost significantly more water before AEs started than lost by samples dehydrated from an initial water potential of about -0.4 MPa. The extra water held by fully hydrated sapwood samples may have been capillary water as defined by Zimmerman.We also report an improved method for the measurement of the ;intensity' of ultrasonic AEs. Intensity is defined here as the area under the positive spikes of the AE signal (plotted as voltage versus time). This method was applied to produce a frequency histogram of the number of AEs versus intensity. A large fraction of the total number of AEs were of low intensity even in small samples (4 mm diameter by 10 mm length). This suggests that the effective ;listening distance' for most AEs was less than 5 to 10 mm.

The ultrasonic-enhanced factor of mass-transfer coefficient in the supercritical carbon dioxide extraction

NASA Astrophysics Data System (ADS)

Luo, Benyi; Lu, Yigang

2008-10-01

Based on several hypotheses about the process of supercritical carbon dioxide extraction, the onflow around the solute granule is figured out by the Navier-Stocks equation. In combination with the Higbie’s solute infiltration model, the link between the mass-transfer coefficient and the velocity of flow is found. The mass-transfer coefficient with the ultrasonical effect is compared with that without the ultrasonical effect, and then a new parameter named the ultrasonic-enhanced factor of mass-transfer coefficient is brought forward, which describes the mathematical model of the supercritical carbon dioxide extraction process enhanced by ultrasonic. The model gives out the relationships among the ultrasonical power, the ultrasonical frequency, the radius of solute granule and the ultrasonic-enhanced factor of mass-transfer coefficient. The results calculated by this model fit well with the experimental data, including the extraction of Coix Lacryma-jobi Seed Oil (CLSO) and Coix Lacryma-jobi Seed Ester (CLSE) from coix seeds and the extraction of Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) from the alga by means of the ultrasonic-enhanced supercritical carbon dioxide extraction (USFE) and the supercritical carbon dioxide extraction (SFE) respectively. This proves the rationality of the ultrasonic-enhanced factor model. The model provides a theoretical basis for the application of ultrasonic-enhanced supercritical fluid extraction technique.

Miniaturized flow injection analysis system

DOEpatents

Folta, J.A.

1997-07-01

A chemical analysis technique known as flow injection analysis is described, wherein small quantities of chemical reagents and sample are intermixed and reacted within a capillary flow system and the reaction products are detected optically, electrochemically, or by other means. A highly miniaturized version of a flow injection analysis system has been fabricated utilizing microfabrication techniques common to the microelectronics industry. The microflow system uses flow capillaries formed by etching microchannels in a silicon or glass wafer followed by bonding to another wafer, commercially available microvalves bonded directly to the microflow channels, and an optical absorption detector cell formed near the capillary outlet, with light being both delivered and collected with fiber optics. The microflow system is designed mainly for analysis of liquids and currently measures 38{times}25{times}3 mm, but can be designed for gas analysis and be substantially smaller in construction. 9 figs.

Stabilization of a capillary bridge far beyond the Rayleigh--Plateau limit using active feedback and acoustic radiation pressure.

NASA Astrophysics Data System (ADS)

Marr-Lyon, Mark J.; Thiessen, David B.; Marston, Philip L.

1997-11-01

A liquid bridge between two solid surfaces is known as a capillary bridge. For a cylindrical bridge in low gravity of radius R and length L, the slenderness S=L/2R has a natural (Rayleigh--Plateau) limit of π beyond which the bridge breaks. Using the radiation pressure of an ultrasonic standing wave to control the shape of the bridge and an optical sensor to detect the shape of the bridge, an active feedback system was constructed that stabilized bridges significantly beyond the Rayleigh limit in simulated low gravity(Marr--Lyon, M. J., phet al., J. Fluid Mech.), accepted for publication.. The Plateau tank which contained the bridge was a dual frequency ultrasonic resonator and the spatial distribution of the radiation pressure was controlled by adjusting the ultrasonic frequency. Bridges have been extended with S as large as 4.3. To be useful in low gravity, modifications for liquid bridges in air are needed. Acoustic resonators in air having the required property that the sound amplitude can be spatially redistributed rapidly are being investigated using gas-filled soap-film bridges. Work supported by NASA.

A FORTRAN program for interpretation of relative permeability from unsteady-state displacements with capillary pressure included

USGS Publications Warehouse

Udegbunam, E.O.

1991-01-01

This paper presents a FORTRAN program for the determination of two-phase relative permeabilities from unsteady-state displacement data with capillary pressure terms included. The interpretative model employed in this program combines the simultaneous solution of a variant of the fractional flow equation which includes a capillary pressure term and an integro-differential equation derived from Darcy's law without assuming the simplified Buckley-Leverett flow. The incorporation of capillary pressure in the governing equations dispenses with the high flowrate experimental requirements normally employed to overcome capillarity effects. An illustrative example is presented herein which implements this program for the determination of oil/water relative permeabilities from a sandstone core sample. Results obtained compares favorably with results previously given in the literature. ?? 1991.

A prototype heat pipe heat exchanger for the capillary pumped loop flight experiment

NASA Technical Reports Server (NTRS)

Ku, Jentung; Yun, Seokgeun; Kroliczek, Edward J.

1992-01-01

A Capillary Pumped Two-Phase Heat Transport Loop (CAPL) Flight Experiment, currently planned for 1993, will provide microgravity verification of the prototype capillary pumped loop (CPL) thermal control system for EOS. CAPL employs a heat pipe heat exchanger (HPHX) to couple the condenser section of the CPL to the radiator assembly. A prototype HPHX consisting of a heat exchanger (HX), a header heat pipe (HHP), a spreader heat pipe (SHP), and a flow regulator has been designed and tested. The HX transmits heat from the CPL condenser to the HHP, while the HHP and SHP transport heat to the radiator assembly. The flow regulator controls flow distribution among multiple parallel HPHX's. Test results indicated that the prototype HPHX could transport up to 800 watts with an overall heat transfer coefficient of more than 6000 watts/sq m-deg C. Flow regulation among parallel HPHX's was also demonstrated.

N2 and CO2 capillary breakthrough experiments on Opalinus Clay

NASA Astrophysics Data System (ADS)

Amann, Alexandra; Busch, Andreas; Krooss, Bernhard M.

2013-04-01

The aim of this project was to identify the critical capillary pressures on the drainage and the imbibition path for clay-rich rocks, at a burial depth of 1500 m (30 MPa confining pressure, 45°C). The experiments were performed on fully water-saturated sample plugs of 38 mm diameter and 5 to 20 mm length. The capillary breakthrough pressure was determined by step-wise increase of the differential pressure (drainage), the capillary snap-off pressure was determined from the final pressure difference at the end of a spontaneous imbibition phase. The confining pressure was kept constant throughout the experiment, which resulted in a continuous change of effective stress. The measurements were performed in a closed system and the pressure response was interpreted in terms of different flow mechanisms (diffusion-controlled vs. viscous flow). In total, four breakthrough experiments with N2 and five experiments with CO2 were conducted. Because of very low flow rates and high critical capillary pressures the experiments took rather long. In some cases the experiments were allowed to run for half a year (drainage experiments). Substantial differences were observed between gas breakthrough (drainage) and snap-off (imbibition) pressures. As expected, breakthrough pressures were always higher than the snap-off pressures. For three samples a pbreakthrough/psnap-off ratio of 1.6 to 1.9 was observed, for one sample a ratio of 4. A clear permeability-capillary pressure relationship could not be identified. Based on (omnidirectional) Hg-injection porosimetry results, and assuming perfectly water wet mineral surfaces, gas breakthrough pressures were predicted to occur at approximately 16 MPa for N2 and 5.7 MPa for CO2. The gas breakthrough experiments, however, produced different results. Even though a relatively homogeneous sample set was chosen, with permeability coefficients ranging between 1E-21 and 6E-21 m², the critical capillary breakthrough pressures for nitrogen ranged

The function profile of compressed-air and ultrasonic nebulizers.

PubMed

Wu, Hsin-Lin; Lin, Yung-Zen; Wu, Wei-Fong; Huang, Fu-Yuan

2003-01-01

In order to study the detailed function of two kinds of nebulizers commonly used in clinical asthma treatment, compressed-air and ultrasonic, this study was conducted. At the beginning, various flow rates were adjusted, paired with different volumes of solutions in the container. The changes of temperature, pH, and osmolality during the course of nebulization were examined. Normal saline, terbutaline, and fenoterol solutions were used as the nebulized solutions. The study was performed in an environment in ambient temperature around 20 degrees C and relative humidity around 70%. The results showed a minimal 6 L/min flow rate was required to nebulize the solution when using the compressed-air nebulizer. The dead volume was about 0.8 ml for compressed-air and 8.5 ml for the ultrasonic nebulizer. When using the compressed-air nebulizer, the temperature, both in the solution and at the mouthpiece site, dropped gradually. On the contrary, the temperatures at both sites increased a little bit when using the ultrasonic nebulizer. The pH values of pure terbutaline and fenoterol nebulized solutions were acidic (3.58 and 3.00 respectively). The osmolality of terbutaline and fenoterol nebulized solutions were isotonic. The osmolality increased gradually during the course of nebulization, to a greater extent in the compressed-air nebulizer. In conclusion, both types of nebulizers have their special features. The ultrasonic nebulizer displays less extent in change of temperature and osmolality during nebulization and is expected to be a better device in treating asthmatic patients in terms of lesser effect on cooling and changing the osmolality of airway mucosa.

Ultrasonic control of ceramic membrane fouling: Effect of particle characteristics.

PubMed

Chen, Dong; Weavers, Linda K; Walker, Harold W

2006-02-01

In this study, the effect of particle characteristics on the ultrasonic control of membrane fouling was investigated. Ultrasound at 20 kHz was applied to a cross-flow filtration system with gamma-alumina membranes in the presence of colloidal silica particles. Experimental results indicated that particle concentration affected the ability of ultrasound to control membrane fouling, with less effective control of fouling at higher particle concentrations. Measurements of sound wave intensity and images of the cavitation region indicated that particles induced additional cavitation bubbles near the ultrasonic source, which resulted in less turbulence reaching the membrane surface and subsequently less effective control of fouling. When silica particles were modified to be hydrophobic, greater inducement of cavitation bubbles near the ultrasonic source occurred for a fixed concentration, also resulting in less effective control of fouling. Particle size influenced the cleaning ability of ultrasound, with better permeate recovery observed with larger particles. Particle size did not affect sound wave intensity, suggesting that the more effective control of fouling by large particles was due to greater lift and cross-flow drag forces on larger particles compared to smaller particles.

From catastrophic acceleration to deceleration of liquid plugs in prewetted capillary tubes

NASA Astrophysics Data System (ADS)

Magniez, Juan; Baudoin, Michael; Zoueshtiagh, Farzam; Lemac/Lics Team

2016-11-01

Liquid/gas flows in capillaries are involved in a multitude of systems including flow in porous media, petroleum extraction, imbibition of paper or flows in pulmonary airways in pathological conditions. Liquid plugs, witch compose the biphasic flows, can have a dramatic impact on patients with pulmonary obstructive diseases, since they considerably alter the circulation of air in the airways and thus can lead to severe breathing difficulties. Here, the dynamics of liquid plugs in prewetted capillary tube is investigated experimentally and theoretically, with a particular emphasis on the role of the prewetting films and of the driving condition (constant flow rate, constant pressure). For both driving conditions, the plugs can either experience a continuous increase or decrease of their size. While this phenomenon is regular in the case of imposed flow rate, a constant pressure head can lead to a catastrophic acceleration of the plug and eventually its rupture or a dramatic increase of the plug size. A theoretical model is proposed to explain the transition between theses two regimes. These results give a new insight on the critical pressure required for airways obstruction and reopening. IEMN, International Laboratory LEMAC/LICS, UMR CNRS 8520, University of Lille.

Dual-wavelength light-emitting diode-based ultraviolet absorption detector for nano-flow capillary liquid chromatography.

PubMed

Xie, Xiaofeng; Tolley, Luke T; Truong, Thy X; Tolley, H Dennis; Farnsworth, Paul B; Lee, Milton L

2017-11-10

The design of a miniaturized LED-based UV-absorption detector was significantly improved for on-column nanoflow LC. The detector measures approximately 27mm×24mm×10mm and weighs only 30g. Detection limits down to the nanomolar range and linearity across 3 orders of magnitude were obtained using sodium anthraquinone-2-sulfonate as a test analyte. Using two miniaturized detectors, a dual-detector system was assembled containing 255nm and 275nm LEDs with only 216nL volume between the detectors A 100μm slit was used for on-column detection with a 150μm i.d. packed capillary column. Chromatographic separation of a phenol mixture was demonstrated using the dual-detector system, with each detector producing a unique chromatogram. Less than 6% variation in the ratios of absorbances measured at the two wavelengths for specific analytes was obtained across 3 orders of magnitude concentration, which demonstrates the potential of using absorption ratio measurements for target analyte detection. The dual-detector system was used for simple, but accurate, mobile phase flow rate measurement at the exit of the column. With a flow rate range from 200 to 2000nL/min, less than 3% variation was observed. Copyright © 2017 Elsevier B.V. All rights reserved.

Influence of local capillary trapping on containment system effectiveness

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bryant, Steven

2014-03-31

extent of LCT thus occurs at P c,entry crit near this threshold. Testing predictions of this simple algorithm against full-physics simulations of buoyancy-driven CO 2 migration support the concept of critical capillary entry pressure. However, further research is needed to determine whether a single value of critical capillary entry pressure always applies and how that value can be determined a priori. Simulations of injection into high-resolution (cells 0.3 m on a side) 2D and 3D heterogeneous domains show two characteristic behaviors. At small gravity numbers (vertical flow velocity much less than horizontal flow velocity) the CO 2 fills local traps as well as regions that would act as local barriers if CO 2 were moving only due to buoyancy. When injection ceases, the CO 2 migrates vertically to establish large saturations within local traps and residual saturation elsewhere. At large gravity numbers, the CO 2 invades a smaller portion of the perforated interval. Within this smaller swept zone the local barriers are not invaded, but local traps are filled to large saturation during injection and remain during post-injection gravity-driven migration. The small gravity number behavior is expected in the region within 100 m of a vertical injection well at anticipated rates of injection for commercial GCS. Simulations of leakage scenarios (through-going region of large permeability imposed in overlying seal) indicate that LCT persists (i.e. CO 2 remains held in a large fraction of the local iv traps) and the persistence is independent of injection rate during storage. Simulations of leakage for the limiting case of CO 2 migrating vertically from an areally extensive emplacement in the lower portion of a reservoir showed similar strong persistence of LCT. This research has two broad implications for GCS. The first is that LCT can retain a significant fraction of the CO 2 stored in a reservoir – above and beyond the residual saturation -- if the overlying seal were to fail

Inorganic plugs removal using ultrasonic waves

NASA Astrophysics Data System (ADS)

Khan, Nasir; Pu, Chunsheng; Xu, Li; Lei, Zhang

2017-03-01

It is essential to recover the lost productivity caused by formation damage in the proximity of the wellbore during different well operations. In comparison to conventionally used methods, the efficiency, reliability, environment friendly, and simple and convenient technique of ultrasonic waves make it more attractive in petroleum industries. In current study, ultrasonic waves were applied to mitigate the formation damage caused by deposition of calcium carbonate (CaCO3) nearby well bore. Results showed that 100 minutes exposure time could efficiently recover 38.1% of original productivity but further increase in irradiation time (120mins) would decrease the recovery to 37.1%. This aberration can be attributed to the particle-bridge formation formed by larger particles at later stages and tendency of acoustic wave to push back the fluid flow. Moreover, ultrasonic waves transducer#2 (Frequency 20KHz and Power 1000W) could recovery maximum recovery of 36.3%, however, high frequency transducer was not effective in this recovery. This inorganic removal can be attributed to the cavitation and thermal energy produced through three different ways including cavitation, boundary friction and transformation upon hitting the medium.

Numerical Simulation of Sickle Cell Blood Flow in the Microcirculation

NASA Astrophysics Data System (ADS)

Berger, Stanley A.; Carlson, Brian E.

2001-11-01

A numerical simulation of normal and sickle cell blood flow through the transverse arteriole-capillary microcirculation is carried out to model the dominant mechanisms involved in the onset of vascular stasis in sickle cell disease. The transverse arteriole-capillary network is described by Strahler's network branching method, and the oxygen and blood transport in the capillaries is modeled by a Krogh cylinder analysis utilizing Lighthill's lubrication theory, as developed by Berger and King. Poiseuille's law is used to represent blood flow in the arterioles. Applying this flow and transport model and utilizing volumetric flow continuity at each network bifurcation, a nonlinear system of equations is obtained, which is solved iteratively using a steepest descent algorithm coupled with a Newton solver. Ten different networks are generated and flow results are calculated for normal blood and sickle cell blood without and with precapillary oxygen loss. We find that total volumetric blood flow through the network is greater in the two sickle cell blood simulations than for normal blood owing to the anemia associated with sickle cell disease. The percentage of capillary blockage in the network increases dramatically with decreasing pressure drop across the network in the sickle cell cases while there is no blockage when normal blood flows through simulated networks. It is concluded that, in sickle cell disease, without any vasomotor dilation response to decreasing oxygen concentrations in the blood, capillary blockage will occur in the microvasculature even at average pressure drops across the transverse arteriole-capillary networks.

Capillary flow weld-bonding

NASA Technical Reports Server (NTRS)

Vaughan, R. W.; Jones, R. J. (Inventor)

1976-01-01

The invention of a weld-bonding technique for titanium plates was described. This involves fastening at least two plates of titanium together using spot-welding and applying a bead of adhesive along the edge of the resistance spot-welded joint which upon heating, flows and fills the separation between the joint components.

Capillaries for use in a multiplexed capillary electrophoresis system

DOEpatents

Yeung, Edward S.; Chang, Huan-Tsang; Fung, Eliza N.

1997-12-09

The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification ("base calling") is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations.

Capillaries for use in a multiplexed capillary electrophoresis system

DOEpatents

Yeung, E.S.; Chang, H.T.; Fung, E.N.

1997-12-09

The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification (``base calling``) is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations. 19 figs.

Application of a spectrally filtered probing light beam and RGB decomposition of microphotographs for flow registration of ultrasonically enhanced agglutination of erythrocytes

NASA Astrophysics Data System (ADS)

Doubrovski, V. A.; Ganilova, Yu. A.; Zabenkov, I. V.

2013-08-01

We propose a development of the flow microscopy method to increase the resolving power upon registration of erythrocyte agglutination. We experimentally show that the action of a ultrasonic standing wave on an agglutinating mixture blood-serum leads to the formation of so large erythrocytic immune complexes that it seems possible to propose a new two-wave optical method of registration of the process of erythrocyte agglutination using the RGB decomposition of microphotographs of the flow of the mixture under study. This approach increases the reliability of registration of erythrocyte agglutination and, consequently, increases the reliability of blood typing. Our results can be used in the development of instruments for automatic human blood typing.

Effects of capillary heterogeneity on vapor-liquid counterflow in porous media

NASA Astrophysics Data System (ADS)

Stubos, A. K.; Satik, C.; Yortsos, Y. C.

1992-06-01

Based on a continuum description, the effect of capillary heterogeneity, induced by variation in permeability, on the steady state, countercurrent, vapor-liquid flow in porous media is analyzed. It is shown that the heterogeneity acts as a body force that may enhance or diminish gravity effects on heat pipes. Selection rules that determine the steady states reached in homogeneous, gravity-driven heat pipes are also formulated. It is shown that the 'infinite' two-phase zone may terminate by a substantial change in the permeability somewhere in the medium. The two possible sequences, liquid-liquid dominated-dry, or liquid-vapor dominated-dry find applications in geothermal systems. Finally, it is shown that although weak heterogeneity affects only gravity controlled flows, stronger variations in permeability can give rise to significant capillary effects.

Analysis of Fractional Flow for Transient Two-Phase Flow in Fractal Porous Medium

NASA Astrophysics Data System (ADS)

Lu, Ting; Duan, Yonggang; Fang, Quantang; Dai, Xiaolu; Wu, Jinsui

2016-03-01

Prediction of fractional flow in fractal porous medium is important for reservoir engineering and chemical engineering as well as hydrology. A physical conceptual fractional flow model of transient two-phase flow is developed in fractal porous medium based on the fractal characteristics of pore-size distribution and on the approximation that porous medium consist of a bundle of tortuous capillaries. The analytical expression for fractional flow for wetting phase is presented, and the proposed expression is the function of structural parameters (such as tortuosity fractal dimension, pore fractal dimension, maximum and minimum diameters of capillaries) and fluid properties (such as contact angle, viscosity and interfacial tension) in fractal porous medium. The sensitive parameters that influence fractional flow and its derivative are formulated, and their impacts on fractional flow are discussed.

Capillary rise and swelling in cellulose sponges

NASA Astrophysics Data System (ADS)

Ha, Jonghyun; Kim, Jungchul; Kim, Ho-Young

2015-11-01

A cellulose sponge, which is a mundane example of a porous hydrophilic structure, can absorb and hold a significant amount of liquid. We present the results of experimental and theoretical investigation of the dynamics of the capillary imbibition of various aqueous solutions in the sponge that swells at the same time. We find that the rate of water rise against the resistance caused by gravitational and viscous effects deviates from Washburn's rule beyond a certain threshold height. We rationalize the novel power law of the rise height versus time by combining Darcy's law with hygroscopic swelling equation and also predict the threshold height. The scaling law constructed through this work agrees well with the experimental results, shedding light on the physics of capillary flow in deforming porous media.

Pericytes as Inducers of Rapid, Matrix Metalloproteinase-9-Dependent Capillary Damage during Ischemia

PubMed Central

Underly, Robert G.; Levy, Manuel; Hartmann, David A.; Grant, Roger I.; Watson, Ashley N.

2017-01-01

Blood–brain barrier disruption (BBB) and release of toxic blood molecules into the brain contributes to neuronal injury during stroke and other cerebrovascular diseases. While pericytes are builders and custodians of the BBB in the normal brain, their impact on BBB integrity during ischemia remains unclear. We imaged pericyte-labeled transgenic mice with in vivo two-photon microscopy to examine the relationship between pericytes and blood plasma leakage during photothrombotic occlusion of cortical capillaries. Upon cessation of capillary flow, we observed that plasma leakage occurred with three times greater frequency in regions where pericyte somata adjoined the endothelium. Pericyte somata covered only 7% of the total capillary length in cortex, indicating that a disproportionate amount of leakage occurred from a small fraction of the capillary bed. Plasma leakage was preceded by rapid activation of matrix metalloproteinase (MMP) at pericyte somata, which was visualized at high resolution in vivo using a fluorescent probe for matrix metalloproteinase-2/9 activity, fluorescein isothiocyanate (FITC)-gelatin. Coinjection of an MMP-9 inhibitor, but not an MMP-2 inhibitor, reduced pericyte-associated FITC-gelatin fluorescence and plasma leakage. These results suggest that pericytes contribute to rapid and localized proteolytic degradation of the BBB during cerebral ischemia. SIGNIFICANCE STATEMENT Pericytes are a key component of the neurovascular unit and are essential for normal BBB function. However, during acute ischemia, we find that pericytes are involved in creating rapid and heterogeneous BBB disruption in the capillary bed. The mechanism by which pericytes contribute to BBB damage warrants further investigation, as it may yield new therapeutic targets for acute stroke injury and other neurological diseases involving capillary flow impairment. PMID:28053036

Zernike ultrasonic tomography for fluid velocity imaging based on pipeline intrusive time-of-flight measurements.

PubMed

Besic, Nikola; Vasile, Gabriel; Anghel, Andrei; Petrut, Teodor-Ion; Ioana, Cornel; Stankovic, Srdjan; Girard, Alexandre; d'Urso, Guy

2014-11-01

In this paper, we propose a novel ultrasonic tomography method for pipeline flow field imaging, based on the Zernike polynomial series. Having intrusive multipath time-offlight ultrasonic measurements (difference in flight time and speed of ultrasound) at the input, we provide at the output tomograms of the fluid velocity components (axial, radial, and orthoradial velocity). Principally, by representing these velocities as Zernike polynomial series, we reduce the tomography problem to an ill-posed problem of finding the coefficients of the series, relying on the acquired ultrasonic measurements. Thereupon, this problem is treated by applying and comparing Tikhonov regularization and quadratically constrained ℓ1 minimization. To enhance the comparative analysis, we additionally introduce sparsity, by employing SVD-based filtering in selecting Zernike polynomials which are to be included in the series. The first approach-Tikhonov regularization without filtering, is used because it is the most suitable method. The performances are quantitatively tested by considering a residual norm and by estimating the flow using the axial velocity tomogram. Finally, the obtained results show the relative residual norm and the error in flow estimation, respectively, ~0.3% and ~1.6% for the less turbulent flow and ~0.5% and ~1.8% for the turbulent flow. Additionally, a qualitative validation is performed by proximate matching of the derived tomograms with a flow physical model.

Direct Numerical Simulations of Dynamic Drainage and Imbibition to Investigate Capillary Pressure-Saturation-Interfacial Area Relation

NASA Astrophysics Data System (ADS)

Konangi, S.; Palakurthi, N. K.; Karadimitriou, N.; Comer, K.; Ghia, U.

2017-12-01

We present results of pore-scale direct numerical simulations (DNS) of drainage and imbibition in a quasi-two-dimensional (40µm thickness) porous medium with a randomly distributed packing of cylindrical obstructions. The Navier-Stokes (NS) equations are solved in the pore space on an Eulerian mesh using the open-source finite-volume computational fluid dynamics (CFD) code, OpenFOAM. The Volume-of-Fluid (VOF) method is employed to track the evolution of the fluid-fluid interface; a static contact angle is used to account for wall adhesion. From the DNS data, we focus on the macroscopic capillary pressure-saturation (Pc-Sw) relation, which is known to be hysteretic, i.e., this relation is flow process (such as drainage, imbibition and scanning curves) and history dependent. In order to overcome the problem of hysteresis, extended theories of multiphase flow hypothesized that the inclusion of specific interfacial area as a state variable will result in a unique relation between capillary pressure, saturation and interfacial area (Pc-Sw-awn). We study the role of specific interfacial area on hysteresis in the macroscopic Pc-Sw relation under non-equilibrium (dynamic) conditions. Under dynamic conditions, capillary pressure depends on the rate of change of the wetting phase saturation, and the dynamic Pc-Sw relation includes the changes caused by viscous effects. Simulations of drainage and imbibition are performed for two capillary numbers by controlling the flow rate of the non-wetting (polydimenthlysiloxane oil) and wetting (water) fluids. From these simulations, the Pc-Sw curves will be estimated; the Pc-S-awn surface will be constructed to determine whether the data points from drainage and imbibition processes fall on a unique surface under transient conditions. Different macroscopic capillary pressure definitions based on phase-averaged pressures and interfacial area will be evaluated. Understanding macroscopic capillary pressure definitions and the uniqueness

Capillary reference half-cell

DOEpatents

Hall, Stephen H.

1996-01-01

The present invention is a reference half-cell electrode wherein intermingling of test fluid with reference fluid does not affect the performance of the reference half-cell over a long time. This intermingling reference half-cell may be used as a single or double junction submersible or surface reference electrode. The intermingling reference half-cell relies on a capillary tube having a first end open to reference fluid and a second end open to test fluid wherein the small diameter of the capillary tube limits free motion of fluid within the capillary to diffusion. The electrode is placed near the first end of the capillary in contact with the reference fluid. The method of operation of the present invention begins with filling the capillary tube with a reference solution. After closing the first end of the capillary, the capillary tube may be fully submerged or partially submerged with the second open end inserted into test fluid. Since the electrode is placed near the first end of the capillary, and since the test fluid may intermingle with the reference fluid through the second open end only by diffusion, this intermingling capillary reference half-cell provides a stable voltage potential for long time periods.

Capillary reference half-cell

DOEpatents

Hall, S.H.

1996-02-13

The present invention is a reference half-cell electrode wherein intermingling of test fluid with reference fluid does not affect the performance of the reference half-cell over a long time. This intermingling reference half-cell may be used as a single or double junction submersible or surface reference electrode. The intermingling reference half-cell relies on a capillary tube having a first end open to reference fluid and a second end open to test fluid wherein the small diameter of the capillary tube limits free motion of fluid within the capillary to diffusion. The electrode is placed near the first end of the capillary in contact with the reference fluid. The method of operation of the present invention begins with filling the capillary tube with a reference solution. After closing the first end of the capillary, the capillary tube may be fully submerged or partially submerged with the second open end inserted into test fluid. Since the electrode is placed near the first end of the capillary, and since the test fluid may intermingle with the reference fluid through the second open end only by diffusion, this intermingling capillary reference half-cell provides a stable voltage potential for long time periods. 11 figs.

Measurement of hydroxyl radical production in ultrasonic aqueous solutions by a novel chemiluminescence method.

PubMed

Hu, Yufei; Zhang, Zhujun; Yang, Chunyan

2008-07-01

Measurement methods for ultrasonic fields are important for reasons of safety. The investigation of an ultrasonic field can be performed by detecting the yield of hydroxyl radicals resulting from ultrasonic cavitations. In this paper, a novel method is introduced for detecting hydroxyl radicals by a chemiluminescence (CL) reaction of luminol-hydrogen peroxide (H2O2)-K5[Cu(HIO6)2](DPC). The yield of hydroxyl radicals is calculated directly by the relative CL intensity according to the corresponding concentration of H2O2. This proposed CL method makes it possible to perform an in-line and real-time assay of hydroxyl radicals in an ultrasonic aqueous solution. With flow injection (FI) technology, this novel CL reaction is sensitive enough to detect ultra trace amounts of H2O2 with a limit of detection (3sigma) of 4.1 x 10(-11) mol L(-1). The influences of ultrasonic output power and ultrasonic treatment time on the yield of hydroxyl radicals by an ultrasound generator were also studied. The results indicate that the amount of hydroxyl radicals increases with the increase of ultrasonic output power (< or = 15 W mL(-1)). There is a linear relationship between the time of ultrasonic treatment and the yield of H2O2. The ultrasonic field of an ultrasonic cleaning baths has been measured by calculating the yield of hydroxyl radicals.

Analytical model for describing ion guiding through capillaries in insulating polymers

NASA Astrophysics Data System (ADS)

Liu, Shi-Dong; Zhao, Yong-Tao; Wang, Yu-Yu; N, Stolterfoht; Cheng, Rui; Zhou, Xian-Ming; Xu, Hu-Shan; Xiao, Guo-Qing

2015-08-01

An analytical description for guiding of ions through nanocapillaries is given on the basis of previous work. The current entering into the capillary is assumed to be divided into a current fraction transmitted through the capillary, a current fraction flowing away via the capillary conductivity and a current fraction remaining within the capillary, which is responsible for its charge-up. The discharging current is assumed to be governed by the Frenkel-Poole process. At higher conductivities the analytical model shows a blocking of the ion transmission, which is in agreement with recent simulations. Also, it is shown that ion blocking observed in experiments is well reproduced by the analytical formula. Furthermore, the asymptotic fraction of transmitted ions is determined. Apart from the key controlling parameter (charge-to-energy ratio), the ratio of the capillary conductivity to the incident current is included in the model. Differences resulting from the nonlinear and linear limits of the Frenkel-Poole discharge are pointed out. Project supported by the Major State Basic Research Development Program of China (Grant No. 2010CB832902) and the National Natural Science Foundation of China (Grant Nos. 11275241, 11275238, 11105192, and 11375034).

Early changes in fiber profile and capillary density in long-term stimulated muscles.

PubMed

Hudlická, O; Dodd, L; Renkin, E M; Gray, S D

1982-10-01

Predominantly fast skeletal muscles of rabbits [tibialis anterior (TA), extensor digitorum longus (EDL)] were stimulated at a frequency naturally occurring in nerves to slow muscles (10 Hz continuously) for 8 h/day for 2--4 days. Such stimulation is known to convert all glycolytic fibers to oxidative and to increase capillary density. Our aim was to study early stages of conversion to investigate the factors responsible for the changes. Staining of quick-frozen sections for myosin ATPase, succinic dehydrogenase, and alkaline phosphatase was used to study the distribution of different fiber types and to measure fiber cross-sectional areas, capillaries per square millimeter, and capillary-to-fiber ratios in each fiber category. TA but not EDL showed conversion of fast glycolytic to fast oxidative fibers after 2 days, more after 4 days of stimulation. In both muscles, the largest fast glycolytic fibers were diminished in number after stimulation. There was significant increase in total capillaries per square millimeter after 4 days and some increase after 2 days of stimulation. The increase in capillaries per square millimeter exceeded the increase in the number of fibers per square millimeter, and since there was no change in mean fiber area, the increase is attributed to capillary growth. In EDL, there was an increase in the number of capillaries supplying both fast glycolytic and fast oxidative fibers, suggesting that capillary growth precedes fiber type conversion. In TA, the number of capillaries supplying fast oxidative fibers was increased but that to fast glycolytic fibers, was not. This is consistent with capillary growth simultaneous with or following fiber conversion. In both TA and EDL the number of capillaries perfused after contraction was higher in stimulated muscles, suggesting that increased capillary flow contributed to capillary growth.

Effect of Capillary Tube’s Shape on Capillary Rising Regime for Viscos Fluids

NASA Astrophysics Data System (ADS)

Soroush, F.; Moosavi, A.

2018-05-01

When properties of the displacing fluid are considered, the rising profile of the penetrating fluid in a capillary tube deviates from its classical Lucas-Washburn profile. Also, shape of capillary tube can affect the rising profile in different aspects. In this article, effect of capillary tube’s shape on the vertical capillary motion in presence of gravity is investigated by considering the properties of the displacing fluid. According to the fact that the differential equation of the capillary rising for a non-simple wall type is very difficult to solve analytically, a finite element simulation model is used for this study. After validation of the simulation model with an experiment that has been done with a simple capillary tube, shape of the capillary tube’s wall is changed in order to understand its effects on the capillary rising and different motion regimes that may appear according to different geometries. The main focus of this article is on the sinusoidal wall shapes and comparing them with a simple wall.

Ultrasonic Motors

DTIC Science & Technology

2003-06-01

micromotor have been investigated. The piezoelectric motor makes use of two orthogonal bending modes of a hollow cylinder. The vibrating element...A.Iino, K.Suzuki, M.Kasuga, M.Suzuki and T.Yamanaka, "Development of a Self- Oscillating Ultrasonic Micromotor and Its Application to a Watch...pp. 823-828, 1997. [12] M. K. Kurosawa, T. Morita, and T. Higuchi, "A Cylindrical Ultrasonic Micromotor Based on PZT Thin Film," IEEE Ultrasonics

Transparent electrodes made with ultrasonic spray coating technique for flexible heaters

NASA Astrophysics Data System (ADS)

Wroblewski, G.; Krzemiński, J.; Janczak, D.; Sowiński, J.; Jakubowska, M.

2017-08-01

Transparent electrodes are one of the basic elements of various electronic components. The paper presents the preliminary results related to novel method of ultrasonic spray coating used for fabrication of transparent flexible electrodes. Experiments were conducted by means of specially made laboratory setup composed of ultrasonic spray generator and XYZ plotter. In the first part of the paper diverse solvents were used to determine the crucial technological parameters such as atomization voltage and fluid flow velocity. Afterwards paint containing carbon nanotubes suspended in the two solvent system was prepared and deposited on the polyethylene terephthalate foil. Thickness, roughness and electrical measurements were performed to designate the relations of technological parameters of ultrasonic spray coating on thickness, roughness, sheet resistance and optical transmission of fabricated samples.

Using evaporation to control capillary instabilities in micro-systems.

PubMed

Ledesma-Aguilar, Rodrigo; Laghezza, Gianluca; Yeomans, Julia M; Vella, Dominic

2017-12-06

The instabilities of fluid interfaces represent both a limitation and an opportunity for the fabrication of small-scale devices. Just as non-uniform capillary pressures can destroy micro-electrical mechanical systems (MEMS), so they can guide the assembly of novel solid and fluid structures. In many such applications the interface appears during an evaporation process and is therefore only present temporarily. It is commonly assumed that this evaporation simply guides the interface through a sequence of equilibrium configurations, and that the rate of evaporation only sets the timescale of this sequence. Here, we use Lattice-Boltzmann simulations and a theoretical analysis to show that, in fact, the rate of evaporation can be a factor in determining the onset and form of dynamical capillary instabilities. Our results shed light on the role of evaporation in previous experiments, and open the possibility of exploiting diffusive mass transfer to directly control capillary flows in MEMS applications.

Protein separation through preliminary experiments concerning pH and salt concentration by tube radial distribution chromatography based on phase separation multiphase flow using a polytetrafluoroethylene capillary tube.

PubMed

Kan, Hyo; Tsukagoshi, Kazuhiko

2017-07-01

Protein mixtures were separated using tube radial distribution chromatography (TRDC) in a polytetrafluoroethylene (PTFE) capillary (internal diameter=100µm) separation tube. Separation by TRDC is based on the annular flow in phase separation multiphase flow and features an open-tube capillary without the use of specific packing agents or application of high voltages. Preliminary experiments were conducted to examine the effects of pH and salt concentration on the phase diagram of the ternary mixed solvent solution of water-acetonitrile-ethyl acetate (8:2:1 volume ratio) and on the TRDC system using the ternary mixed solvent solution. A model protein mixture containing peroxidase, lysozyme, and bovine serum albumin was analyzed via TRDC with the ternary mixed solvent solution at various pH values, i.e., buffer-acetonitrile-ethyl acetate (8:2:1 volume ratio). Protein was separated on the chromatograms by the TRDC system, where the elution order was determined by the relation between the isoelectric points of protein and the pH values of the solvent solution. Copyright © 2017 Elsevier B.V. All rights reserved.

Harnessing electrical forces for separation. Capillary zone electrophoresis, isoelectric focusing, field-flow fractionation, split-flow thin-cell continuous-separation and other techniques.

PubMed

Giddings, J C

1989-10-20

A simple analysis, first presented twenty years ago, showed that the effectiveness of a field-driven separation like electrophoresis, as expressed by the maximum number of theoretical plates (N), is given by the dimensionless ratio of two energies N = -delta mu ext/2RT in which -delta mu ext is the electrical potential energy drop of a charged species and RT is the thermal energy (R is the gas constant and T is the absolute temperature). Quantity -delta mu ext is the product of the force F acting on the species and the path length X of separation. The exceptional power of electrophoresis, for which often N approximately 10(6), can be traced directly to the enormous magnitude of the electrical force F. This paper explores the fundamentals underlying several different means for utilizing these powerful electrical forces for separation, including capillary zone electrophoresis, gel electrophoresis, isoelectric focusing, electrical field-flow fractionation and split-flow thin continuous separation cells. Remarkably, the above equation and its relatives are found to describe the approximate performance of all these diverse electrically driven systems. Factors affecting both the resolving power and separation speed of the systems are addressed; from these considerations some broad optimization criteria emerge. The capabilities of the different methods are compared using numerical examples.

Biodegradation of phenol, salicylic acid, benzenesulfonic acid, and iomeprol by Pseudomonas fluorescens in the capillary fringe

NASA Astrophysics Data System (ADS)

Hack, Norman; Reinwand, Christian; Abbt-Braun, Gudrun; Horn, Harald; Frimmel, Fritz H.

2015-12-01

Mass transfer and biological transformation phenomena in the capillary fringe were studied using phenol, salicylic acid, benzenesulfonic acid, and the iodinated X-ray contrast agent iomeprol as model organic compounds and the microorganism strain Pseudomonas fluorescens. Three experimental approaches were used: Batch experiments (uniform water saturation and transport by diffusion), in static columns (with a gradient of water saturation and advective transport in the capillaries) and in a flow-through cell (with a gradient of water saturation and transport by horizontal and vertical flow: 2-dimension flow-through microcosm). The reactors employed for the experiments were filled with quartz sand of defined particle size distribution (dp = 200…600 μm, porosity ε = 0.42). Batch experiments showed that phenol and salicylic acid have a high, whereas benzenesulfonic acid and iomeprol have a quite low potential for biodegradation under aerobic conditions and in a matrix nearly close to water saturation. Batch experiments under anoxic conditions with nitrate as electron acceptor revealed that the biodegradation of the model compounds was lower than under aerobic conditions. Nevertheless, the experiments showed that the moisture content was also responsible for an optimized transport in the liquid phase of a porous medium. Biodegradation in the capillary fringe was found to be influenced by both the moisture content and availability of the dissolved substrate, as seen in static column experiments. The gas-liquid mass transfer of oxygen also played an important role for the biological activity. In static column experiments under aerobic conditions, the highest biodegradation was found in the capillary fringe (e.g. βt/β0 (phenol) = 0 after t = 6 d) relative to the zone below the water table and unsaturated zone. The highest biodegradation occurred in the flow-through cell experiment where the height of the capillary fringe was largest.

Biodegradation of phenol, salicylic acid, benzenesulfonic acid, and iomeprol by Pseudomonas fluorescens in the capillary fringe.

PubMed

Hack, Norman; Reinwand, Christian; Abbt-Braun, Gudrun; Horn, Harald; Frimmel, Fritz H

2015-12-01

Mass transfer and biological transformation phenomena in the capillary fringe were studied using phenol, salicylic acid, benzenesulfonic acid, and the iodinated X-ray contrast agent iomeprol as model organic compounds and the microorganism strain Pseudomonas fluorescens. Three experimental approaches were used: Batch experiments (uniform water saturation and transport by diffusion), in static columns (with a gradient of water saturation and advective transport in the capillaries) and in a flow-through cell (with a gradient of water saturation and transport by horizontal and vertical flow: 2-dimension flow-through microcosm). The reactors employed for the experiments were filled with quartz sand of defined particle size distribution (dp=200...600 μm, porosity ε=0.42). Batch experiments showed that phenol and salicylic acid have a high, whereas benzenesulfonic acid and iomeprol have a quite low potential for biodegradation under aerobic conditions and in a matrix nearly close to water saturation. Batch experiments under anoxic conditions with nitrate as electron acceptor revealed that the biodegradation of the model compounds was lower than under aerobic conditions. Nevertheless, the experiments showed that the moisture content was also responsible for an optimized transport in the liquid phase of a porous medium. Biodegradation in the capillary fringe was found to be influenced by both the moisture content and availability of the dissolved substrate, as seen in static column experiments. The gas-liquid mass transfer of oxygen also played an important role for the biological activity. In static column experiments under aerobic conditions, the highest biodegradation was found in the capillary fringe (e.g. βt/β0 (phenol)=0 after t=6 d) relative to the zone below the water table and unsaturated zone. The highest biodegradation occurred in the flow-through cell experiment where the height of the capillary fringe was largest. Copyright © 2015 Elsevier B.V. All rights

A microfluidic flow injection system for DNA assay with fluids driven by an on-chip integrated pump based on capillary and evaporation effects.

PubMed

Xu, Zhang-Run; Zhong, Chong-Hui; Guan, Yan-Xia; Chen, Xu-Wei; Wang, Jian-Hua; Fang, Zhao-Lun

2008-10-01

A miniaturized flow injection analysis (FIA) system integrating a micropump on a microfluidic chip based on capillary and evaporation effects was developed. The pump was made by fixing a filter paper plug with a vent tube at the channel end, it requires no peripheral equipment and provides steady flow in the microl min(-1) range for FIA operation. Valve-free sample injection was achieved at nanolitre level using an array of slotted vials. The practical applicability of the system was demonstrated by DNA assay with laser-induced fluorescence (LIF) detection. A precision of 1.6% RSD (10.0 ng microl(-1), n=15) was achieved with a sampling throughput of 76 h(-1) and sample consumption of 95 nl.

Ultrasonic Blood Flow Measurement in Haemodialysis

PubMed Central

Sampson, D.; Papadimitriou, M.; Kulatilake, A. E.

1970-01-01

A 5-megacycle Doppler flow meter, calibrated in-vitro, was found to give a linear response to blood flow in the ranges commonly encountered in haemodialysis. With this, blood flow through artificial kidneys could be measured simply and with a clinically acceptable error. The method is safe, as blood lines do not have to be punctured or disconnected and hence there is no risk of introducing infection. Besides its value as a research tool the flow meter is useful in evaluating new artificial kidneys. Suitably modified it could form the basis of an arterial flow alarm system. PMID:5416812

Velocity dependence of biphasic flow structuration: steady-state and oscillating flow effects

NASA Astrophysics Data System (ADS)

Tore Tallakstad, Ken; Jankov, Mihailo; Løvoll, Grunde; Toussaint, Renaud; Jørgen Mâløy, Knut; Grude Flekkøy, Eirik; Schmittbuhl, Jean; Schäfer, Gerhard; Méheust, Yves; Arendt Knudsen, Henning

2010-05-01

We study various types of biphasic flows in quasi-two-dimensional transparent porous models. These flows imply a viscous wetting fluid, and a lowly viscous one. The models are transparent, allowing the displacement process and structure to be monitored in space and time. Three different aspects will be presented: 1. In stationary biphasic flows, we study the relationship between the macroscopic pressure drop (related to relative permeability) and the average flow rate, and how this arises from the cluster size distribution of the lowly viscous fluid [1]. 2. In drainage situations, we study how the geometry of the invader can be explained, and how it gives rise to apparent dynamic capillary effects. We show how these can be explained by viscous effects on evolving geometries of invading fluid [2]. 3. We study the impact of oscillating pressure fields superimposed to a background flow over the flow regimes patterns [3]. Steady-State Two-Phase Flow in Porous Media: Statistics and Transport Properties. First, in stationary flow with a control of the flux of both fluids, we show how the pressure drop depends on the flow rate. We will show that the dynamics is dominated by the interplay between a viscous pressure field from the wetting fluid and bubble transport of a less viscous, nonwetting phase. In contrast with more studied displacement front systems, steady-state flow is in equilibrium, statistically speaking. The corresponding theoretical simplicity allows us to explain a data collapse in the cluster size distribution of lowly viscous fluid in the system, as well as the relation |?P|∞√Ca--. This allows to explain so called relative permeability effects by the morphological changes of the cluster size distribution. Influence of viscous fingering on dynamic saturation-pressure curves in porous media. Next, we study drainage in such models, and investigate the relationship between the pressure field and the morphology of the invading fluid. This allows to model

Microscopic Measurements of Axial Accumulation of Red Blood Cells in Capillary Flows Effects of Deformability

NASA Astrophysics Data System (ADS)

Sasaki, Takahiro; Seki, Junji; Itano, Tomoaki; Sugihara-Seki, Masako

2017-11-01

In the microcirculation, red blood cells (RBCs) are known to accumulate in the region near the central axis of microvessels, which is called the ``axial accumulation''. Although this behavior of RBCs is considered to originate from high deformability of RBCs, there have been few experimental studies on the mechanism. In order to elucidate the effect of RBC deformability on the axial accumulation, we measured the cross-sectional distributions of RBCs flowing through capillary tubes with a high spatial resolution by a newly devised observation system for intact and softened RBCs as well as hardened RBCs to various degrees. It was found that the intact and softened RBCs are concentrated in the small area centered on the tube axis, whereas the hardened RBCs are dispersed widely over the tube cross section dependent on the degree of hardness. These results demonstrate clearly the essential role of the deformability of RBCs in the ``axial accumulation'' of RBCs. JSPS KAKENHI Grant Number 17H03176, Kansai University ORDIST group funds.

Highly sensitive chiral analysis in capillary electrophoresis with large-volume sample stacking with an electroosmotic flow pump.

PubMed

Kawai, Takayuki; Koino, Hiroshi; Sueyoshi, Kenji; Kitagawa, Fumihiko; Otsuka, Koji

2012-07-13

To improve the sensitivity in chiral analysis by capillary electrophoresis without loss of optical resolution, application of large-volume sample stacking with an electroosmotic flow pump (LVSEP) was investigated. Effects of the addition of cyclodextrin (CD) into a running solution on the LVSEP preconcentration was theoretically studied, where the preconcentration efficiency and effective separation length would be slightly increased if the effective electrophoretic velocity (v(ep,eff,BGS)) of the analytes was decreased by interacting with CD. In LVSEP-CD-modified capillary zone electrophoresis (CDCZE) and LVSEP-CD electrokinetic chromatography with reduced v(ep,eff,BGS), up to 1000-fold sensitivity increases were achieved with almost no loss of resolution. In LVSEP-CD-modified micellar electrokinetic chromatography of amino acids with increased v(ep,eff,BGS), a 1300-fold sensitivity increase was achieved without much loss of resolution, indicating the versatile applicability of LVSEP to many separation modes. An enantio-excess (EE) assay was also carried out in LVSEP-CDCZE, resulting in successful analyses of up to 99.6% EE. Finally, we analyzed ibuprofen in urine by desalting with a C₁₈ solid-phase extraction column. As a typical result, 250ppb ibuprofen was well concentrated and optically resolved with 84.0-86.6% recovery in LVSEP-CDCZE, indicating the applicability of LVSEP to real samples containing a large amount of unnecessary background salts. Copyright © 2012 Elsevier B.V. All rights reserved.

Genesis of the Mechanical Heart Valves' Ultrasonic Closing Clicks

NASA Astrophysics Data System (ADS)

Hasegawa, Jun; Kobayashi, Kenji

A new in vitro experimental tool was developed to study the mechanism of the ultrasonic closing clicks' genesis of mechanical heart valves. Since the newly developed tester adopted compressed air flow directly instead of the blood analog fluid to drive the mechanical heart valve, it is not possibe to generate any cavitation. Closing clicks were measured with a small accelerometer at the surface of the valve holder made of silicone rubber. Ultrasonic closing clicks as well as audible closing clicks, similar to those measured clinically, could be observed using this setup. Thus, it was confirmed that the ultrasonic closing clicks can be generated without the existence of cavitation. Simultaneous measurements of the valve motion were made with a high-speed video camera, and the analysis of the video frames and clicks showed that higher frequency signal components of more than 50kHz could be generated only at the instant of the closure, which means the collision of the occluder with the housing. Eighteen miniature accelerometers with an area of one square millimeter were developed and stuck on the housing to monitor the distribution of the housing vibrations in detail, and it was found that the vibrations correspond to the ultrasonic closing clicks propagated from the valve stop: the collision point of the occluder with the housing. This fact indicated that the generation of ultrasonic closing clicks are limited to the small area of the collision. From those results, it was concluded that the major origin of the ultrasonic closing clicks' genesis should be the collision of the occluder with the housing.

Scale-dependent coupling of hysteretic capillary pressure, trapping, and fluid mobilities

NASA Astrophysics Data System (ADS)

Doster, F.; Celia, M. A.; Nordbotten, J. M.

2012-12-01

Many applications of multiphase flow in porous media, including CO2-storage and enhanced oil recovery, require mathematical models that span a large range of length scales. In the context of numerical simulations, practical grid sizes are often on the order of tens of meters, thereby de facto defining a coarse model scale. Under particular conditions, it is possible to approximate the sub-grid-scale distribution of the fluid saturation within a grid cell; that reconstructed saturation can then be used to compute effective properties at the coarse scale. If both the density difference between the fluids and the vertical extend of the grid cell are large, and buoyant segregation within the cell on a sufficiently shorte time scale, then the phase pressure distributions are essentially hydrostatic and the saturation profile can be reconstructed from the inferred capillary pressures. However, the saturation reconstruction may not be unique because the parameters and parameter functions of classical formulations of two-phase flow in porous media - the relative permeability functions, the capillary pressure -saturation relationship, and the residual saturations - show path dependence, i.e. their values depend not only on the state variables but also on their drainage and imbibition histories. In this study we focus on capillary pressure hysteresis and trapping and show that the contribution of hysteresis to effective quantities is dependent on the vertical length scale. By studying the transition from the two extreme cases - the homogeneous saturation distribution for small vertical extents and the completely segregated distribution for large extents - we identify how hysteretic capillary pressure at the local scale induces hysteresis in all coarse-scale quantities for medium vertical extents and finally vanishes for large vertical extents. Our results allow for more accurate vertically integrated modeling while improving our understanding of the coupling of capillary

Label-free in vivo optical micro-angiography imaging of cerebral capillary blood flow within meninges and cortex in mice with the skull left intact

NASA Astrophysics Data System (ADS)

Jia, Yali; Wang, Ruikang K.

2011-03-01

Abnormal microcirculation within meninges is common in many neurological diseases. There is a need for an imaging method that is capable of visualizing functional meningeal microcirculations alone, preferably decoupled from the cortical blood flow. Optical microangiography (OMAG) is a recently developed label-free imaging method capable of producing 3D images of dynamic blood perfusion within micro-circulatory tissue beds at an imaging depth up to ~2 mm, with an unprecedented imaging sensitivity to the blood flow at ~4 μm/s. In this study, we demonstrate the utility of ultra-high sensitive OMAG in imaging the detailed blood flow distributions, at a capillary level resolution, within meninges and cortex in mice with the cranium left intact. The results indicate that OMAG can be a valuable tool for the study of meningeal circulations.

Capillary Structures for Exploration Life Support (Capillary Structures)

NASA Image and Video Library

2017-07-10

iss052e013081 (7/10/2017) --- The Capillary Structures for Exploration Life Support (Capillary Structures) investigation studies a new method using structures of specific shapes to manage fluid and gas mixtures. The investigation studies water recycling and carbon dioxide removal, benefiting future efforts to design lightweight, more reliable life support systems for future space missions.

Effects of sonication radiation on oil recovery by ultrasonic waves stimulated water-flooding.

PubMed

Mohammadian, Erfan; Junin, Radzuan; Rahmani, Omeid; Idris, Ahmad Kamal

2013-02-01

Due to partial understanding of mechanisms involved in application of ultrasonic waves as enhanced oil recovery method, series of straight (normal), and ultrasonic stimulated water-flooding experiments were conducted on a long unconsolidated sand pack using ultrasonic transducers. Kerosene, vaseline, and SAE-10 (engine oil) were used as non-wet phase in the system. In addition, a series of fluid flow and temperature rise experiments were conducted using ultrasonic bath in order to enhance the understanding about contributing mechanisms. 3-16% increase in the recovery of water-flooding was observed. Emulsification, viscosity reduction, and cavitation were identified as contributing mechanisms. The findings of this study are expected to increase the insight to involving mechanisms which lead to improving the recovery of oil as a result of application of ultrasound waves. Copyright © 2012 Elsevier B.V. All rights reserved.

Crossover from capillary fingering to viscous fingering in a rough fracture

NASA Astrophysics Data System (ADS)

Hu, R.; Chen, Y.; Wu, D. S.

2017-12-01

Controlled by the competition between capillary and viscous forces, the displacement patterns of one fluid displacing another more viscous one exhibit capillary fingering, viscous fingering, and the crossover between the two. Although extensive studies have investigated viscous and capillary fingerings in porous and fractured media, a few studies focused on the crossover in rough fractures, and how viscous and capillary forces affect the crossover remains unclear. Using a transparent fracture visualization system, we studied how the competition impacts the crossover in a horizontal rough fracture. Drainage experiments of water displacing oil were conducted at seven flow rates (capillary number log10Ca ranging from -7.07 to -3.07) and four viscosity ratios (M = 1/1000, 1/500, 1/100 and 1/50). We consistently observed lower invading fluid saturations in the crossover zone. In addition, we proposed a phase diagram for the displacement patterns in a rough fracture that is consistent with similar studies in porous media. Based on real-time imaging and statistical analysis of the invasion morphology, we showed that the competition between the capillary and viscous forces is responsible for the saturation reduction in the crossover zone. In this zone, finger propagation toward the outlet (characteristic of viscous fingering) as well as void-filling in the transverse and backward directions (characteristic of capillary fingering), are both suppressed. Therefore, the invading fluid tends to occupy larger apertures with higher characteristic front velocity, promoting void-filling toward the outlet with thinner finger growth and resulting in a larger volume of defending fluid left behind.

Development of ultrasonic atomizer and its application to S. I. engines

DOE Office of Scientific and Technical Information (OSTI.GOV)

Namiyama, K.; Nakamura, H.; Kokubo, K.

1989-01-01

This paper describes a fuel atomizer developed for S.I. engines based on ultrasonic vibrations. As the spray is characterized by fine droplet size and low penetration, it facilitates fuel movement and the formation of a homogeneous mixture. The spray behavior of this atomizer is easily influenced by ambient air motion. Therefore, the spray is most effectively delivered to the cylinders by precise injection timing. The ultrasonic atomizer disperses a fine spray over a wide flow rate range. A single cylinder engine fitted with the atomizer showed advantages in combustion speed and transient response performance.

High-power ultrasonic processing: Recent developments and prospective advances

NASA Astrophysics Data System (ADS)

Gallego-Juarez, Juan A.

2010-01-01

Although the application of ultrasonic energy to produce or to enhance a wide variety of processes have been explored since about the middle of the 20th century, only a reduced number of ultrasonic processes have been established at industrial level. However, during the last ten years the interest in ultrasonic processing has revived particularly in industrial sectors where the ultrasonic technology may represent a clean and efficient tool to improve classical existing processes or an innovation alternative for the development of new processes. Such seems to be the case of relevant sectors such as food industry, environment, pharmaceuticals and chemicals manufacture, machinery, mining, etc where power ultrasound is becoming an emerging technology for process development. The possible major problem in the application of high-intensity ultrasound on industrial processing is the design and development of efficient power ultrasonic systems (generators and reactors) capable of large scale successful operation specifically adapted to each individual process. In the area of ultrasonic processing in fluid media and more specifically in gases, the development of the steppedplate transducers and other power ge with extensive radiating surface has strongly contributed to the implementation at semi-industrial and industrial stage of several commercial applications, in sectors such as food and beverage industry (defoaming, drying, extraction, etc), environment (air cleaning, sludge filtration, etc...), machinery and process for manufacturing (textile washing, paint manufacture, etc). The development of different cavitational reactors for liquid treatment in continuous flow is helping to introduce into industry the wide potential of the area of sonochemistry. Processes such as water and effluent treatment, crystallization, soil remediation, etc have been already implemented at semi-industrial and/or industrial stage. Other single advances in sectors like mining or energy have

Capillary electrophoresis/mass spectrometry determination of inorganic ions using an ion spray-sheath flow interface.

PubMed

Huggins, T G; Henion, J D

1993-01-01

The determination of inorganic cations and anions by capillary electrophoresis/mass spectrometry (CE/MS) is reported using an ion spray-sheath flow interface coupling. A twelve-component synthetic mixture of cations which included the positive ions of K, Ba, Ca, Mn, Cd, Co, Pb, Cr, Ni, Zn, Ag, and Cu was loaded into the capillary column at levels ranging from 30 to 300 pg, separated by CE, and detected by indirect UV and in the full-scan (m/z 35-450) positive ion CE/MS mode using an aqueous buffer containing 30 mM creatinine and 8 mM alpha-hydroxyisobutyric acid, pH 4.8. Creatinine forms adducts with the cations which are observed in the gas phase and requires rather high (120 electron volts) declustering energy to dissociate. This produces a reduction in charge state to form the free, singly charged, inorganic cations which are observed in the mass spectra. CE/MS analysis of an aqueous acidic extract of used aircraft engine oil revealed high levels of lead as well as lower levels of chromium and nickel. CE-indirect UV analysis of a synthetic mixture containing 300 pg each of 11 inorganic ions, which included the anions of Br, Cl, NO2, NO3, S2O3, N3, SCN, SO4, SeO4, oxalate, and MoO4, is shown. The running buffer which affected this separation contained 5 mM ammonium dichromate, 10 mM ammonium acetate, and 20 mM diethylenetriamine at pH 9.3. Although indirect UV detection revealed good separation of these anions, CE/MS analysis of this mixture was complicated by interfering ion current signals from the cluster ions formed by the interaction between the additives and the analytes.(ABSTRACT TRUNCATED AT 250 WORDS)

Laminar and turbulent surgical plume characteristics generated from curved- and straight-blade laparoscopic ultrasonic dissectors.

PubMed

Kim, Fernando J; Sehrt, David; Pompeo, Alexandre; Molina, Wilson R

2014-05-01

To characterize laparoscopic ultrasonic dissector surgical plume emission (laminar or turbulent) and investigate plume settlement time between curved and straight blades. A straight and a curved blade laparoscopic ultrasonic dissector were activated on tissue and in a liquid environment to evaluate plume emission. Plume emission was characterized as either laminar or turbulent and the plume settlement times were compared. Devices were then placed in liquid to observed consistency in the fluid disruption. Two types of plume emission were identified generating different directions of plume: laminar flow causes minimal visual obstruction by directing the aerosol downwards, while turbulent flow directs plume erratically across the cavity. Laminar plume dissipates immediately while turbulent plume reaches a second maximum obstruction approximately 0.3 s after activation and clears after 2 s. Turbulent plume was observed with the straight blade in 10 % of activations, and from the curved blade in 47 % of activations. The straight blade emitted less obstructive plume. Turbulent flow is disruptive to laparoscopic visibility with greater field obstruction and requires longer settling than laminar plume. Ultrasonic dissectors with straight blades have more consistent oscillations and generate more laminar flow compared with curved blades. Surgeons may avoid laparoscope smearing from maximum plume generation depending on blade geometry.

Effect of surfactant on kinetics of thinning of capillary bridges

NASA Astrophysics Data System (ADS)

Nowak, Emilia; Kovalchuk, Nina; Simmons, Mark

2015-11-01

Kinetics of thinning of capillary bridges is of great scientific and industrial interest being of vital importance for example in various emulsification and microfluidic processes. It is well known that the rate of bridge thinning is proportional to the interfacial tension. Therefore it is expected that the process should slow down by addition of surfactant. The kinetics of capillary bridges in the presence of surfactant was studied by the dripping of liquid from a capillary tip under conditions of nearly zero flow rate (We << 1). The tested liquids were aqueous solutions of sodium lauryl ether sulphate (SLES), which is broadly used in personal care products. The viscosity, surfactant activity and adsorption kinetics have been controlled by addition of glycerol and sodium chloride. The study has shown that the kinetics of capillary bridges are determined by dynamic surface tension rather than by its equilibrium value. In particular, the kinetics of the bridge thinning for the 0.1 g L-1 aqueous SLES solution is practically the same as that of pure water despite twice lower equilibrium surface tension. EPSRC Programme Grant, MEMPHIS, EP/K0039761/1.

Gold nanoparticle incorporated inverse opal photonic crystal capillaries for optofluidic surface enhanced Raman spectroscopy.

PubMed

Zhao, Xiangwei; Xue, Jiangyang; Mu, Zhongde; Huang, Yin; Lu, Meng; Gu, Zhongze

2015-10-15

Novel transducers are needed for point of care testing (POCT) devices which aim at facile, sensitive and quick acquisition of health related information. Recent advances in optofluidics offer tremendous opportunities for biological/chemical analysis using extremely small sample volumes. This paper demonstrates nanostructured capillary tubes for surface enhanced Raman spectroscopy (SERS) analysis in a flow-through fashion. The capillary tube integrates the SERS sensor and the nanofluidic structure to synergistically offer sample delivery and analysis functions. Inside the capillary tube, inverse opal photonic crystal (IO PhC) was fabricated using the co-assembly approach to form nanoscale liquid pathways. In the nano-voids of the IO PhC, gold nanoparticles were in situ synthesized and functioned as the SERS hotspots. The advantages of the flow-through SERS sensor are multifold. The capillary effect facilities the sample delivery process, the nanofluidic channels boosts the interaction of analyte and gold nanoparticles, and the PhC structure strengthens the optical field near the SERS hotspots and results in enhanced SERS signals from analytes. As an exemplary demonstration, the sensor was used to measure creatinein spiked in artificial urine samples with detection limit of 0.9 mg/dL. Copyright © 2015 Elsevier B.V. All rights reserved.

Automated Parallel Capillary Electrophoretic System

DOEpatents

Li, Qingbo; Kane, Thomas E.; Liu, Changsheng; Sonnenschein, Bernard; Sharer, Michael V.; Kernan, John R.

2000-02-22

An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.

Investigation and visualization of liquid-liquid flow in a vertically mounted Hele-Shaw cell: flow regimes, velocity and shape of droplets

NASA Astrophysics Data System (ADS)

Shad, S.; Gates, I. D.; Maini, B. B.

2009-11-01

The motion and shape of a liquid drop flowing within a continuous, conveying liquid phase in a vertical Hele-Shaw cell were investigated experimentally. The continuous phase was more viscous and wetted the bounding walls of the Hele-Shaw cell. The gap between the Hele-Shaw plates was set equal to 0.0226 cm. Four different flow regimes were observed: (a) small-droplet flow, (b) elongated-droplet flow, (c) churn flow and (d) channel flow. At low capillary number, that is, when capillary forces are larger than viscous forces, the droplet shape was irregular and changed with time and distance, and it moved with lower velocity than that of the conveying phase. At higher capillary number, several different shapes of stabilized elongated and flattened drops were observed. In contrast to gas-liquid systems, the velocities of droplets are higher than that of conveying liquid. New correlations derived from dimensionless analysis and fitted to the experimental data were generated to predict the elongated-drop velocity and aspect ratio.

Dependence of Capillary Properties of Contemporary Clinker Bricks on Their Microstructure

NASA Astrophysics Data System (ADS)

Wesołowska, Maria; Kaczmarek, Anna

2017-10-01

Contemporary clinker bricks are applied for outer layers of walls built from other materials and walls which should have high durability and aesthetic qualities. The intended effect depends not only on the mortar applied but also on clinker properties. Traditional macroscopic tests do not allow to predict clinker behaviour in contact with mortars and external environment. The basic information for this issue is open porosity of material. It defines the material ability to absorb liquids: rain water (through the face wall surface) and grout from mortar (through base surface). The main capillary flow goes on in pores with diameters from 300 to 3000nm. It is possible to define pore distribution and their size using the Mercury Intrusion Porosimetry method. The aim of these research is evaluation of clinker brick capillary properties (initial water absorption and capillary rate) and analysis of differences in microstructure of the face and base wall of a product. Detailed results allowed to show pore distribution in function of their diameters and definition of pore amount responsible for capillary flow. Based on relation between volume function differential and pore diameter, a differential distribution curve was obtained which helped to determine the dominant diameters. The results obtained let us state that face wall of bricks was characterized with the lowest material density and open porosity. In this layer (most burnt) part of pores could be closed by locally appearing liquid phase during brick burning. Thus density is lower comparing to other part of the product.

Ultrasonic Maintenance

NASA Technical Reports Server (NTRS)

1991-01-01

The Ultraprobe 2000, manufactured by UE Systems, Inc., Elmsford, NY, is a hand-held ultrasonic system that detects indications of bearing failure by analyzing changes in amplitude. It employs the technology of a prototype ultrasonic bearing-failure monitoring system developed by Mechanical Technology, Inc., Latham, New York and Marshall Space Flight Center (which was based on research into Skylab's gyroscope bearings). Bearings on the verge of failure send ultrasonic signals indicating their deterioration; the Ultraprobe changes these to audible signals. The operator hears the signals and gages their intensity with a meter in the unit.

Effect of ultrasonic stimulation on particle transport and fate over different lengths of porous media

NASA Astrophysics Data System (ADS)

Chen, Xingxin; Wu, Zhonghan; Cai, Qipeng; Cao, Wei

2018-04-01

It is well established that seismic waves traveling through porous media stimulate fluid flow and accelerate particle transport. However, the mechanism remains poorly understood. To quantify the coupling effect of hydrodynamic force, transportation distance, and ultrasonic stimulation on particle transport and fate in porous media, laboratory experiments were conducted using custom-built ultrasonic-controlled soil column equipment. Three column lengths (23 cm, 33 cm, and 43 cm) were selected to examine the influence of transportation distance. Transport experiments were performed with 0 W, 600 W, 1000 W, 1400 W, and 1800 W of applied ultrasound, and flow rates of 0.065 cm/s, 0.130 cm/s, and 0.195 cm/s, to establish the roles of ultrasonic stimulation and hydrodynamic force. The laboratory results suggest that whilst ultrasonic stimulation does inhibit suspended-particle deposition and accelerate deposited-particle release, both hydrodynamic force and transportation distance are the principal controlling factors. The median particle diameter for the peak concentration was approximately 50% of that retained in the soil column. Simulated particle-breakthrough curves using extended traditional filtration theory effectively described the experimental curves, particularly the curves that exhibited a higher tailing concentration.

Development of a flow controller for long-term sampling of gases and vapors using evacuated canisters.

PubMed

Rossner, Alan; Farant, Jean Pierre; Simon, Philippe; Wick, David P

2002-11-15

Anthropogenic activities contribute to the release of a wide variety of volatile organic compounds (VOC) into microenvironments. Developing and implementing new air sampling technologies that allow for the characterization of exposures to VOC can be useful for evaluating environmental and health concerns arising from such occurrences. A novel air sampler based on the use of a capillary flow controller connected to evacuated canisters (300 mL, 1 and 6 L) was designed and tested. The capillary tube, used to control the flow of air, is a variation on a sharp-edge orifice flow controller. It essentially controls the velocity of the fluid (air) as a function of the properties of the fluid, tube diameter and length. A model to predict flow rate in this dynamic system was developed. The mathematical model presented here was developed using the Hagen-Poiseuille equation and the ideal gas law to predict flow into the canisters used to sample for long periods of time. The Hagen-Poiseuille equation shows the relationship between flow rate, pressure gradient, capillary resistance, fluid viscosity, capillary length and diameter. The flow rates evaluated were extremely low, ranging from 0.05 to 1 mL min(-1). The model was compared with experimental results and was shown to overestimate the flow rate. Empirical equations were developed to more accurately predict flow for the 300 mL, 1 and 6 L canisters used for sampling periods ranging from several hours to one month. The theoretical and observed flow rates for different capillary geometries were evaluated. Each capillary flow controller geometry that was tested was found to generate very reproducible results, RSD < 2%. Also, the empirical formulas developed to predict flow rate given a specified diameter and capillary length were found to predict flow rate within 6% of the experimental data. The samplers were exposed to a variety of airborne vapors that allowed for comparison of the effectiveness of capillary flow controllers to

Effect of Ultrasonic Frequency on Lactic Acid Fermentation Promotion by Ultrasonic Irradiation

NASA Astrophysics Data System (ADS)

Shimada, Tadayuki; Ohdaira, Etsuzo; Masuzawa, Nobuyoshi

2004-05-01

The authors have been researching the promotion of lactic acid fermentation by ultrasonic irradiation. In the past research, it was proven that ultrasonic irradiation is effective in the process of fermentation, and the production of yoghurt and kefir was promoted. In this study, the effect of the ultrasonic frequency in this fermentation process was examined. In the frequency range of this study, it was found that the action of fermentation promotion was exponentially proportionate to the irradiated ultrasonic frequency.

Electrically modulated capillary filling imbibition of nematic liquid crystals

NASA Astrophysics Data System (ADS)

Dhar, Jayabrata; Chakraborty, Suman

2018-04-01

The flow of nematic liquid crystals (NLCs) in the presence of an electric field is typically characterized by the variation in its rheological properties due to transition in its molecular arrangements. Here, we bring out a nontrivial interplay of a consequent alteration in the resistive viscous effects and driving electrocapillary interactions, toward maneuvering the capillary filling dynamics over miniaturized scales. Considering a dynamic interplay of the relevant bulk and interfacial forces acting in tandem, our results converge nicely to previously reported experimental data. Finally, we attempt a scaling analysis to bring forth further insight to the reported observations. Our analysis paves the way for the development of microfluidic strategies with previously unexplored paradigms of interaction between electrical and fluidic phenomenon, providing with an augmented controllability on capillary filling as compared to tthose reported to be achievable by the existing strategies. This, in turn, holds utilitarian scopes in improved designs of functional capillarities in electro-optical systems, electrorheological utilities, electrokinetic flow control, as well as in interfacing and imaging systems for biomedical microdevices.

Capillary liquid chromatography-microchip atmospheric pressure chemical ionization-mass spectrometry.

PubMed

Ostman, Pekka; Jäntti, Sirkku; Grigoras, Kestas; Saarela, Ville; Ketola, Raimo A; Franssila, Sami; Kotiaho, Tapio; Kostiainen, Risto

2006-07-01

A miniaturized nebulizer chip for capillary liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (capillary LC-microchip APCI-MS) is presented. The APCI chip consists of two wafers, a silicon wafer and a Pyrex glass wafer. The silicon wafer has a DRIE etched through-wafer nebulizer gas inlet, an edge capillary insertion channel, a stopper, a vaporizer channel and a nozzle. The platinum heater electrode and pads for electrical connection were patterned on to the Pyrex glass wafer. The two wafers were joined by anodic bonding, creating a microchip version of an APCI-source. The sample inlet capillary from an LC column is directly connected to the vaporizer channel of the APCI chip. The etched nozzle in the microchip forms a narrow sample plume, which is ionized by an external corona needle, and the formed ions are analyzed by a mass spectrometer. The nebulizer chip enables for the first time the use of low flow rate separation techniques with APCI-MS. The performance of capillary LC-microchip APCI-MS was tested with selected neurosteroids. The capillary LC-microchip APCI-MS provides quantitative repeatability and good linearity. The limits of detection (LOD) with a signal-to-noise ratio (S/N) of 3 in MS/MS mode for the selected neurosteroids were 20-1000 fmol (10-500 nmol l(-1)). LODs (S/N = 3) with commercial macro APCI with the same compounds using the same MS were about 10 times higher. Fast heat transfer allows the use of the optimized temperature for each compound during an LC run. The microchip APCI-source provides a convenient and easy method to combine capillary LC to any API-MS equipped with an APCI source. The advantages and potentials of the microchip APCI also make it a very attractive interface in microfluidic APCI-MS.

Ultrasonic probe deployment device for increased wave transmission and rapid area scan inspections

DOEpatents

DiMambro, Joseph; Roach, Dennis P; Rackow, Kirk A; Nelson, Ciji L; Dasch, Cameron J; Moore, David G

2013-02-12

An ultrasonic probe deployment device in which an ultrasound-transmitting liquid forms the portion of the ultrasonic wave path in contact with the surface being inspected (i.e., the inspection surface). A seal constrains flow of the liquid, for example preventing the liquid from surging out and flooding the inspection surface. The seal is not rigid and conforms to variations in the shape and unevenness of the inspection surface, thus forming a seal (although possibly a leaky seal) around the liquid. The probe preferably is held in place to produce optimum ultrasonic focus on the area of interest. Use of encoders can facilitate the production of C-scan area maps of the material being inspected.

Ultrasonic probe deployment device for increased wave transmission and rapid area scan inspections

DOEpatents

DiMambro, Joseph [Placitas, NM; Roach, Dennis P [Albuquerque, NM; Rackow, Kirk A [Albuquerque, NM; Nelson, Ciji L [Albuquerque, NM; Dasch, Cameron J [Boomfield Hills, MI; Moore, David G [Albuquerque, NM

2012-01-03

An ultrasonic probe deployment device in which an ultrasound-transmitting liquid forms the portion of the ultrasonic wave path in contact with the surface being inspected (i.e., the inspection surface). A seal constrains flow of the liquid, for example preventing the liquid from surging out and flooding the inspection surface. The seal is not rigid and conforms to variations in the shape and unevenness of the inspection surface, thus forming a seal (although possibly a leaky seal) around the liquid. The probe preferably is held in place to produce optimum ultrasonic focus on the area of interest. Use of encoders can facilitate the production of C-scan area maps of the material being inspected.

Ultrasonic speech translator and communications system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Akerman, M.A.; Ayers, C.W.; Haynes, H.D.

1996-07-23

A wireless communication system undetectable by radio frequency methods for converting audio signals, including human voice, to electronic signals in the ultrasonic frequency range, transmitting the ultrasonic signal by way of acoustical pressure waves across a carrier medium, including gases, liquids, or solids, and reconverting the ultrasonic acoustical pressure waves back to the original audio signal. The ultrasonic speech translator and communication system includes an ultrasonic transmitting device and an ultrasonic receiving device. The ultrasonic transmitting device accepts as input an audio signal such as human voice input from a microphone or tape deck. The ultrasonic transmitting device frequency modulatesmore » an ultrasonic carrier signal with the audio signal producing a frequency modulated ultrasonic carrier signal, which is transmitted via acoustical pressure waves across a carrier medium such as gases, liquids or solids. The ultrasonic receiving device converts the frequency modulated ultrasonic acoustical pressure waves to a frequency modulated electronic signal, demodulates the audio signal from the ultrasonic carrier signal, and conditions the demodulated audio signal to reproduce the original audio signal at its output. 7 figs.« less

Ultrasonic speech translator and communications system

DOEpatents

Akerman, M.A.; Ayers, C.W.; Haynes, H.D.

1996-07-23

A wireless communication system undetectable by radio frequency methods for converting audio signals, including human voice, to electronic signals in the ultrasonic frequency range, transmitting the ultrasonic signal by way of acoustical pressure waves across a carrier medium, including gases, liquids, or solids, and reconverting the ultrasonic acoustical pressure waves back to the original audio signal. The ultrasonic speech translator and communication system includes an ultrasonic transmitting device and an ultrasonic receiving device. The ultrasonic transmitting device accepts as input an audio signal such as human voice input from a microphone or tape deck. The ultrasonic transmitting device frequency modulates an ultrasonic carrier signal with the audio signal producing a frequency modulated ultrasonic carrier signal, which is transmitted via acoustical pressure waves across a carrier medium such as gases, liquids or solids. The ultrasonic receiving device converts the frequency modulated ultrasonic acoustical pressure waves to a frequency modulated electronic signal, demodulates the audio signal from the ultrasonic carrier signal, and conditions the demodulated audio signal to reproduce the original audio signal at its output. 7 figs.

Ultrasonic speech translator and communications system

DOEpatents

Akerman, M. Alfred; Ayers, Curtis W.; Haynes, Howard D.

1996-01-01

A wireless communication system undetectable by radio frequency methods for converting audio signals, including human voice, to electronic signals in the ultrasonic frequency range, transmitting the ultrasonic signal by way of acoustical pressure waves across a carrier medium, including gases, liquids, or solids, and reconverting the ultrasonic acoustical pressure waves back to the original audio signal. The ultrasonic speech translator and communication system (20) includes an ultrasonic transmitting device (100) and an ultrasonic receiving device (200). The ultrasonic transmitting device (100) accepts as input (115) an audio signal such as human voice input from a microphone (114) or tape deck. The ultrasonic transmitting device (100) frequency modulates an ultrasonic carrier signal with the audio signal producing a frequency modulated ultrasonic carrier signal, which is transmitted via acoustical pressure waves across a carrier medium such as gases, liquids or solids. The ultrasonic receiving device (200) converts the frequency modulated ultrasonic acoustical pressure waves to a frequency modulated electronic signal, demodulates the audio signal from the ultrasonic carrier signal, and conditions the demodulated audio signal to reproduce the original audio signal at its output (250).

Evaluation of the effect of elevated intraocular pressure and reduced ocular perfusion pressure on retinal capillary bed filling and total retinal blood flow in rats by OMAG/OCT.

PubMed

Zhi, Zhongwei; Cepurna, William; Johnson, Elaine; Jayaram, Hari; Morrison, John; Wang, Ruikang K

2015-09-01

To determine if retinal capillary filling is preserved in the face of acutely elevated intraocular pressure (IOP) in anesthetized rats, despite a reduction in total retinal blood flow (RBF), using optical microangiography/optical coherence tomography (OMAG/OCT). OMAG provided the capability of depth-resolved imaging of the retinal microvasculature down to the capillary level. Doppler OCT was applied to measure the total RBF using an enface integration approach. The microvascular pattern, capillary density, and total RBF were monitored in vivo as the IOP was increased from 10 to 100mmHg in 10mmHg intervals and returned back to 10mmHg. In animals with mean arterial pressure (MAP) of 102±4mmHg (n=10), when IOP was increased from 0 to 100mmHg, the capillary density remained at or above 80% of baseline for the IOP up to 60mmHg [or ocular perfusion pressure (OPP) at 40mmHg]. This was then decreased, achieving 60% of baseline at IOP 70mmHg and OPP of 30mmHg. Total RBF was unaffected by moderate increases in IOP up to 30mmHg, beyond which total RBF decreased linearly, reaching 50% of baseline at IOP 60mmHg and OPP 40mmHg. Both capillary density and total RBF were totally extinguished at 100mmHg, but fully recovered when IOP returned to baseline. By comparison, a separate group of animals with lower MAP (mean=75±6mmHg, n=7) demonstrated comparable decreases in both capillary filling and total RBF at IOPs that were 20mmHg lower than in the initial group. Both were totally extinguished at 80mmHg, but fully recovered when IOP returned to baseline. Relationships of both parameters to OPP were unchanged. Retinal capillary filling and total RBF responses to IOP elevation can be monitored non-invasively by OMAG/OCT and both are influenced by OPP. Retinal capillary filling was relatively preserved down to a perfusion pressure of 40mmHg, despite a linear reduction in total RBF. Copyright © 2015 Elsevier Inc. All rights reserved.

Flow equation for porous plug and capillary tube flow restrictors

NASA Technical Reports Server (NTRS)

Davis, W. S.

1972-01-01

Development of flow measuring apparatus for determining low flow performance of resistojet thruster is discussed. Diagram of test equipment is presented. Operation of test equipment is described and numerical relationships are explained.