Ultraviolet Spectral Irradiance Scale Comparison: 210 nm to 300 nm

PubMed Central

Thompson, Ambler; Early, Edward A.; O’Brian, Thomas R.

1998-01-01

Comparison of the irradiances from a number of ultraviolet spectral irradiance standards, based on different physical principles, showed agreement to within their combined standard uncertainties as assigned to them by NIST. The wavelength region of the spectral irradiance comparison was from 210 nm to 300 nm. The spectral irradiance sources were: an electron storage ring, 1000 W quartz-halogen lamps, deuterium arc lamps, and a windowless argon miniarc. PMID:28009378

Use of zero order diffraction of a grating monochromator towards convenient and sensitive detection of fluorescent analytes in multi fluorophoric systems

NASA Astrophysics Data System (ADS)

Panigrahi, Suraj Kumar; Mishra, Ashok Kumar

2018-02-01

White light excitation fluorescence (WLEF) is known to possess analytical advantage in terms of enhanced sensitivity and facile capture of the entire fluorescence spectral signature of multi component fluorescence systems. Using the zero order diffraction of the grating monochromator on the excitation side of a commercial spectrofluorimeter, it has been shown that WLEF spectral measurements can be conveniently carried out. Taking analyte multi-fluorophoric systems like (i) drugs and vitamins spiked in urine sample, (ii) adulteration of extra virgin olive oil with olive pomace oil and (iii) mixture of fabric dyes, it was observed that there is a significant enhancement of measurement sensitivity. The total fluorescence spectral response could be conveniently analysed using PLS2 regression. This work brings out the ease of the use of a conventional fluorimeter for WLEF measurements.

The Copernicus ultraviolet spectral atlas Tau Scorpii

NASA Technical Reports Server (NTRS)

Rogerson, J. B., Jr.; Upson, W. L., II

1977-01-01

An ultraviolet spectral atlas was presented for the B0 V star, Tau Scorpii. It was scanned from 949 to 1560 A by the Princeton spectrometer aboard the Copernicus satellite. From 949 to 1420 A the observations have a nominal resolution of 0.05 A. At the longer wavelengths, the resolution was 0.1 A. The atlas was presented in both tables and graphs.

Spectral transmission of the pig lens: effect of ultraviolet A+B radiation.

PubMed

Artigas, C; Navea, A; López-Murcia, M-M; Felipe, A; Desco, C; Artigas, J-M

2014-12-01

To determine the spectral transmission curve of the crystalline lens of the pig. To analyse how this curve changes when the crystalline lens is irradiated with ultraviolet A+B radiation similar to that of the sun. To compare these results with literature data from the human crystalline lens. We used crystalline lenses of the common pig from a slaughterhouse, i.e. genetically similar pigs, fed with the same diet, and slaughtered at six months old. Spectral transmission was measured with a Perkin-Elmer Lambda 35 UV/VIS spectrometer. The lenses were irradiated using an Asahi Spectra Lax-C100 ultraviolet source, which made it possible to select the spectral emission band as well as the intensity and exposure time. The pig lens transmits all the visible spectrum (95%) and lets part of the ultraviolet A through (15%). Exposure to acute UV (A+B) irradiation causes a decrease in its transmission as the intensity or exposure time increases: this decrease is considerable in the UV region. We were able to determine the mean spectral transmission curve of the pig lens. It appears to be similar to that of the human lens in the visible spectrum, but different in the ultraviolet. Pig lens transmission is reduced by UV (A+B) irradiation and its transmission in the UV region can even disappear as the intensity or exposure time increases. An adequate exposure intensity and time of UV (A+B) radiation always causes an anterior subcapsular cataract (ASC). Copyright © 2014. Published by Elsevier Masson SAS.

Design of an ultraviolet fluorescence lidar for biological aerosol detection

NASA Astrophysics Data System (ADS)

Rao, Zhimin; Hua, Dengxin; He, Tingyao; Le, Jing

2016-09-01

In order to investigate the biological aerosols in the atmosphere, we have designed an ultraviolet laser induced fluorescence lidar based on the lidar measuring principle. The fluorescence lidar employs a Nd:YAG laser of 266 nm as an excited transmitter, and examines the intensity of the received light at 400 nm for biological aerosol concentration measurements. In this work, we firstly describe the designed configuration and the simulation to estimate the measure range and the system resolution of biological aerosol concentration under certain background radiation. With a relative error of less than 10%, numerical simulations show the system is able to monitor biological aerosols within detected distances of 1.8 km and of 7.3 km in the daytime and nighttime, respectively. Simulated results demonstrate the designed fluorescence lidar is capable to identify a minimum concentration of biological aerosols at 5.0×10-5 ppb in the daytime and 1.0×10-7 ppb in the nighttime at the range of 0.1 km. We believe the ultraviolet laser induced fluorescence lidar can be spread in the field of remote sensing of biological aerosols in the atmosphere.

Hyperspectral small animal fluorescence imaging: spectral selection imaging

NASA Astrophysics Data System (ADS)

Leavesley, Silas; Jiang, Yanan; Patsekin, Valery; Hall, Heidi; Vizard, Douglas; Robinson, J. Paul

2008-02-01

Molecular imaging is a rapidly growing area of research, fueled by needs in pharmaceutical drug-development for methods for high-throughput screening, pre-clinical and clinical screening for visualizing tumor growth and drug targeting, and a growing number of applications in the molecular biology fields. Small animal fluorescence imaging employs fluorescent probes to target molecular events in vivo, with a large number of molecular targeting probes readily available. The ease at which new targeting compounds can be developed, the short acquisition times, and the low cost (compared to microCT, MRI, or PET) makes fluorescence imaging attractive. However, small animal fluorescence imaging suffers from high optical scattering, absorption, and autofluorescence. Much of these problems can be overcome through multispectral imaging techniques, which collect images at different fluorescence emission wavelengths, followed by analysis, classification, and spectral deconvolution methods to isolate signals from fluorescence emission. We present an alternative to the current method, using hyperspectral excitation scanning (spectral selection imaging), a technique that allows excitation at any wavelength in the visible and near-infrared wavelength range. In many cases, excitation imaging may be more effective at identifying specific fluorescence signals because of the higher complexity of the fluorophore excitation spectrum. Because the excitation is filtered and not the emission, the resolution limit and image shift imposed by acousto-optic tunable filters have no effect on imager performance. We will discuss design of the imager, optimizing the imager for use in small animal fluorescence imaging, and application of spectral analysis and classification methods for identifying specific fluorescence signals.

Oil leakage detection for electric power equipment based on ultraviolet fluorescence effect

NASA Astrophysics Data System (ADS)

Zhang, Jing; Wang, Jian-hui; Xu, Bin; Huang, Zhi-dong; Huang, Lan-tao

2018-03-01

This paper presents a method to detect the oil leakage of high voltage power equipment based on ultraviolet fluorescence effect. The method exploits the principle that the insulating oil has the fluorescent effect under the irradiation of specific ultraviolet light. The emission spectrum of insulating oil under excitation light with different wavelengths is measured and analyzed first. On this basis, a portable oil leakage detective device for high voltage power equipment is designed and developed with a selected 365 nm ultraviolet as the excitation light and the low light level camera as the fluorescence image collector. Then, the feasibility of the proposed method and device in different conditions is experimentally verified in the laboratory environment. Finally, the developed oil leakage detective device is applied to 500 kV Xiamen substation and Quanzhou substation. And the results show that the device can detect the oil leakage of high voltage electrical equipment quickly and conveniently even under the condition of a slight oil leakage especially in the low light environment.

Spectral and Temporal Laser Fluorescence Analysis Such as for Natural Aquatic Environments

NASA Technical Reports Server (NTRS)

Chekalyuk, Alexander (Inventor)

2015-01-01

An Advanced Laser Fluorometer (ALF) can combine spectrally and temporally resolved measurements of laser-stimulated emission (LSE) for characterization of dissolved and particulate matter, including fluorescence constituents, in liquids. Spectral deconvolution (SDC) analysis of LSE spectral measurements can accurately retrieve information about individual fluorescent bands, such as can be attributed to chlorophyll-a (Chl-a), phycobiliprotein (PBP) pigments, or chromophoric dissolved organic matter (CDOM), among others. Improved physiological assessments of photosynthesizing organisms can use SDC analysis and temporal LSE measurements to assess variable fluorescence corrected for SDC-retrieved background fluorescence. Fluorescence assessments of Chl-a concentration based on LSE spectral measurements can be improved using photo-physiological information from temporal measurements. Quantitative assessments of PBP pigments, CDOM, and other fluorescent constituents, as well as basic structural characterizations of photosynthesizing populations, can be performed using SDC analysis of LSE spectral measurements.

International Ultraviolet Explorer (IUE) ultraviolet spectral atlas of selected astronomical objects

NASA Technical Reports Server (NTRS)

Wu, Chi-Chao; Reichert, Gail A.; Ake, Thomas B.; Boggess, Albert; Holm, Albert V.; Imhoff, Catherine L.; Kondo, Yoji; Mead, Jaylee M.; Shore, Steven N.

1992-01-01

The IUE Ultraviolet Spectral Atlas of Selected Astronomical Objects (or 'the Atlas'), is based on the data that were available in the IUE archive in 1986, and is intended to be a quick reference for the ultraviolet spectra of many categories of astronomical objects. It shows reflected sunlight from the Moon, planets, and asteroids, and also shows emission from comets. Comprehensive compilations of UV spectra for main sequence, subgiant, giant, bright giant, and supergiant stars are published elsewhere. This Atlas contains the spectra for objects occupying other areas of the Hertzsprung-Russell diagram: pre-main sequence stars, chemically peculiar stars, pulsating variables, subluminous stars, and Wolf-Rayet stars. This Atlas also presents phenomena such as the chromospheric and transition region emissions from late-type stars; composite spectra of stars, gas streams, accretion disks and gas envelopes of binary systems; the behavior of gas ejecta shortly after the outburst of novac and supernovac; and the H II regions, planetary nebulae, and supernova remnants. Population 2 stars, globular clusters, and luminous stars in the Magellanic Clouds, M31, and M33, are also included in this publication. Finally, the Atlas gives the ultraviolet spectra of galaxies of different Hubble types and of active galaxies.

Spectral Calibration of the MSFC Solar Ultraviolet Magnetograph

NASA Technical Reports Server (NTRS)

West, Edward; Kobayashi, Ken; Cirtain, Jonathan; Gary, Allen; Davis, John; Reader, Joseph

2009-01-01

This paper describes the scientific goals of a sounding rocket program called the Solar Ultraviolet Magnetograph Investigation (SUMI), presents a brief description of the optics that were developed to meet those goals and discusses the spectral, spatial and polarization characteristics of SUMI's Toroidal Variable-Line-Space (TVLS) gratings; which are critical to SUMI's measurements of the magnetic field in the Sun's transition region.

Ultraviolet fluorescence to identify navel oranges with poor peel quality and decay

USDA-ARS?s Scientific Manuscript database

Navel oranges were sorted into four groups under ultraviolet (UV) illumination in commercial packinghouse black light rooms based upon the amount of fluorescence visible on each fruit to determine if fluorescence was predictive of peel quality. The groups corresponded to fruit with: 1) no fluorescen...

A compact multi-channel fluorescence sensor with ambient light suppression

NASA Astrophysics Data System (ADS)

Egly, Dominik; Geörg, Daniel; Rädle, Matthias; Beuermann, Thomas

2012-03-01

A multi-channel fluorescence sensor has been developed for process monitoring and fluorescence diagnostics. It comprises a fiber-optic set-up with an immersion probe and an intensity-modulated high power ultraviolet light-emitting diode as a light source for fluorescence excitation. By applying an electronic lock-in procedure, fluorescence signals are selectively detectable at ambient light levels of 1000 000 times higher intensity. The sensor was designed to be compact, low cost and easily adaptable to a wide field of application. The set-up was used to simultaneously monitor three important metabolic fluorophores: NAD(P)H, flavins and porphyrins during the cultivation of a baker's yeast. Moreover, the accumulation and degradation kinetics of protoporphyrin IX induced by 5-aminolevulinic acid on the skin could be recorded by the sensor. The detection limit for protoporphyrin IX was determined to be 4 × 10-11 mol L-1. The linear signal amplification of the sensor and time courses of fluorescence signals monitored during yeast fermentations were validated using a commercial CCD spectrometer. The robust and flexible set-up of the fiber-optic measurement system promises easy implementation of this non-invasive analytical tool to fluorescence monitoring and diagnostics in R&D and production.

The Copernicus ultraviolet spectral atlas of Gamma Pegasi

NASA Technical Reports Server (NTRS)

Rogerson, J. B., Jr.

1985-01-01

An ultraviolet spectral atlas is presented for the B2 IV star Gamma Pegasi, which has been scanned from 970 to 1501 A by the Princeton spectrometer aboard the Copernicus satellite. From 970 to 1430 A the observations have a nominal resolution of 0.05 A. At the longer wavelengths the resolution is 0.1 A. The atlas is presented in graphs. Line identifications are also listed.

The Copernicus ultraviolet spectral atlas of Tau Scorpii

NASA Technical Reports Server (NTRS)

Rogerson, J. B., Jr.; Upson, W. L., II

1977-01-01

An ultraviolet spectral atlas is presented for the B0 V star, Tau Scorpii. It has been scanned from 949 to 1560 A by the Princeton spectrometer aboard the Copernicus satellite. From 949 to 1420 A the observations have a nominal resolution of 0.05 A. At the longer wavelengths, the resolution is 0.1 A. The atlas is presented in both tables and graphs.

Multi-Spectral Solar Telescope Array. IV - The soft X-ray and extreme ultraviolet filters

NASA Technical Reports Server (NTRS)

Lindblom, Joakim F.; O'Neal, Ray H.; Walker, Arthur B. C., Jr.; Powell, Forbes R.; Barbee, Troy W., Jr.; Hoover, Richard B.

1991-01-01

NASA's Multi-Spectral Solar Telescope Array uses various combinations of thin foil filters composed of Al, C, Te, Be, Mo, Rh, and phthalocyanine to achieve the requisite radiation-rejection characteristics. Such rejection is demanded by the presence of strong EUV radiation at longer wavelengths where the specular reflectivity of multilayer mirrors can cause 'contamination' of the image in the narrow band defined by the Bragg condition.

Microscopic fluorescence spectral analysis of basal cell carcinomas

NASA Astrophysics Data System (ADS)

He, Qingli; Lui, Harvey; Zloty, David; Cowan, Bryce; Warshawski, Larry; McLean, David I.; Zeng, Haishan

2007-05-01

Background and Objectives. Laser-induced autofluorescence (LIAF) is a promising tool for cancer diagnosis. This method is based on the differences in autofluorescence spectra between normal and cancerous tissues, but the underlined mechanisms are not well understood. The objective of this research is to study the microscopic origins and intrinsic fluorescence properties of basal cell carcinoma (BCC) for better understanding of the mechanism of in vivo fluorescence detection and margin delineation of BCCs on skin patients. A home-made micro- spectrophotometer (MSP) system was used to image the fluorophore distribution and to measure the fluorescence spectra of various microscopic structures and regions on frozen tissue sections. Materials and Methods. BCC tissue samples were obtained from 14 patients undergoing surgical resections. After surgical removal, each tissue sample was immediately embedded in OCT medium and snap-frozen in liquid nitrogen. The frozen tissue block was then cut into 16-μm thickness sections using a cryostat microtome and placed on microscopic glass slides. The sections for fluorescence study were kept unstained and unfixed, and then analyzed by the MSP system. The adjacent tissue sections were H&E stained for histopathological examination and also served to help identify various microstructures on the adjacent unstained sections. The MSP system has all the functions of a conventional microscope, plus the ability of performing spectral analysis on selected micro-areas of a microscopic sample. For tissue fluorescence analysis, 442nm He-Cd laser light is used to illuminate and excite the unstained tissue sections. A 473-nm long pass filter was inserted behind the microscope objective to block the transmitted laser light while passing longer wavelength fluorescence signal. The fluorescence image of the sample can be viewed through the eyepieces and also recorded by a CCD camera. An optical fiber is mounted onto the image plane of the photograph

The Copernicus ultraviolet spectral atlas of Beta Orionis

NASA Technical Reports Server (NTRS)

Rogerson, J. B., Jr.; Upson, W. L., II

1982-01-01

An ultraviolet spectral atlas is presented for the B8 Ia star Beta Orionis, which has been scanned from 999 to 1561 A by the Princeton spectrometer aboard the Copernicus satellite. From 999 to 1420 A the observations have a nominal resolution of 0.05 A. At the longer wavelengths the resolution is 0.1 A. The atlas is presented in graphs. Lines identified in the spectrum are also listed.

The Copernicus ultraviolet spectral atlas of Iota Herculis

NASA Technical Reports Server (NTRS)

Upson, W. L., II; Rogerson, J. B., Jr.

1980-01-01

An ultraviolet spectral atlas is presented for the B3 IV star Iota Herculis, which has been scanned from 999 to 1467 A by the Princeton spectrometer aboard the Copernicus satellite. From 999 to 1422 A the observations have a nominal resolution of 0.05 A. At the longer wavelengths the resolution is 0.1 A. The atlas is presented in graphs. Lines identified in the spectrum are also listed.

Potential applications of near infrared auto-fluorescence spectral polarized imaging for assessment of food quality

NASA Astrophysics Data System (ADS)

Zhou, Kenneth J.; Chen, Jun

2016-03-01

The current growing of food industry for low production costs and high efficiency needs for maintenance of high-quality standards and assurance of food safety while avoiding liability issues. Quality and safety of food depend on physical (texture, color, tenderness etc.), chemical (fat content, moisture, protein content, pH, etc.), and biological (total bacterial count etc.) features. There is a need for a rapid (less than a few minutes) and accurate detection system in order to optimize quality and assure safety of food. However, the fluorescence ranges for known fluorophores are limited to ultraviolet emission bands, which are not in the tissue near infrared (NIR) "optical window". Biological tissues excited by far-red or NIR light would exhibit strong emission in spectral range of 650-1,100 nm although no characteristic peaks show the emission from which known fluorophores. The characteristics of the auto-fluorescence emission of different types of tissues were found to be different between different tissue components such as fat, high quality muscle food. In this paper, NIR auto-fluorescence emission from different types of muscle food and fat was measured. The differences of fluorescence intensities of the different types of muscle food and fat emissions were observed. These can be explained by the change of the microscopic structure of physical, chemical, and biological features in meat. The difference of emission intensities of fat and lean meat tissues was applied to monitor food quality and safety using spectral polarized imaging, which can be detect deep depth fat under the muscle food up to several centimeter.

Performance of fluorescence retrieval methods and fluorescence spectrum reconstruction under various sensor spectral configurations

NASA Astrophysics Data System (ADS)

Li, Rong; Zhao, Feng

2015-10-01

Solar-induced chlorophyll fluorescence is closely related to photosynthesis and can serve as an indicator of plant status. Several methods have been proposed to retrieve fluorescence signal (Fs) either at specific spectral bands or within the whole fluorescence emission region. In this study, we investigated the precision of the fluorescence signal obtained through these methods under various sensor spectral characteristics. Simulated datasets generated by the SCOPE (Soil Canopy Observation, Photochemistry and Energy fluxes) model with known `true' Fs as well as an experimental dataset are exploited to investigate four commonly used Fs retrieval methods, namely the original Fraunhofer Line Discriminator method (FLD), the 3 bands FLD (3FLD), the improved FLD (iFLD), and the Spectral Fitting Methods (SFMs). Fluorescence Spectrum Reconstruction (FSR) method is also investigated using simulated datasets. The sensor characteristics of spectral resolution (SR) and signal-to-noise ratio (SNR) are taken into account. According to the results, finer SR and SNR both lead to better accuracy. Lowest precision is obtained for the FLD method with strong overestimation. Some improvements are made by the 3FLD method, but it still tends to overestimate. Generally, the iFLD method and the SFMs provide better accuracy. As to FSR, the shape and magnitude of reconstructed Fs are generally consistent with the `true' Fs distributions when fine SR is exploited. With coarser SR, however, though R2 of the retrieved Fs may be high, large bias is likely to be obtained as well.

Method for determining surface coverage by materials exhibiting different fluorescent properties

NASA Technical Reports Server (NTRS)

Chappelle, Emmett W. (Inventor); Daughtry, Craig S. T. (Inventor); Mcmurtrey, James E., III (Inventor)

1995-01-01

An improved method for detecting, measuring, and distinguishing crop residue, live vegetation, and mineral soil is presented. By measuring fluorescence in multiple bands, live and dead vegetation are distinguished. The surface of the ground is illuminated with ultraviolet radiation, inducing fluorescence in certain molecules. The emitted fluorescent emission induced by the ultraviolet radiation is measured by means of a fluorescence detector, consisting of a photodetector or video camera and filters. The spectral content of the emitted fluorescent emission is characterized at each point sampled, and the proportion of the sampled area covered by residue or vegetation is calculated.

Ultraviolet Opacity and Fluorescence in Supernova Envelopes

NASA Technical Reports Server (NTRS)

Li, Hongwei; McCray, Richard

1996-01-01

By the time the expanding envelope of a Type 2 supernova becomes transparent in the optical continuum, most of the gamma-ray luminosity produced by radioactive Fe/Co/Ni clumps propagates into the hydrogen/helium envelope and is deposited there, if at all. The resulting fast electrons excite He 1 and H 1, the two- photon continua of which are the dominant internal sources of ultraviolet radiation. The UV radiation is blocked by scattering in thousands of resonance lines of metals and converted by fluorescence into optical and infrared emission lines that escape freely. We describe results of Monte Carlo calculations that simulate non-LTE scattering and fluorescence in more than five million allowed lines of Ca, Sc, Ti, V, Cr, Mn, Fe, Co, and Ni. For a model approximating conditions in the envelope of SN 1987A, the calculated emergent spectrum resembles the observed one. For the first 2 yr after explosion, the ultraviolet radiation (lambda less than or approximately equals 3000) is largely blocked and converted into a quasi continuum of many thousands of weak optical and infrared emission lines and some prominent emission features, such as the Ca 2 lambdalambda8600 triplet. Later, as the envelope cools and expands, it becomes more transparent, and an increasing fraction of the luminosity emerges in the UV band.

STUDIES ON BIOLUMINESCENCE : XVII. FLUORESCENCE AND INHIBITION OF LUMINESCENCE IN CTENOPHORES BY ULTRA-VIOLET LIGHT.

PubMed

Harvey, E N

1925-01-20

1. Small dumps of the luminous cells of Mnemiopsis cannot readily be stimulated mechanically but will luminesce on treatment with saponin solution. Larger groups of luminous cells (such as are connected with two paddle plates) luminesce on mechanical stimulation. This suggests that mechanical stimulation to luminesce occurs chiefly through a nerve mechanism which has been broken up in the small dumps of luminous tissue. 2. The smallest bits of luminous tissue, even cells freed from the animal by agitation, that will pass through filter paper, lose their power to luminesce in daylight and regain it (at least partially) in the dark. 3. Luminescence of the whole animal and of individual cells is suppressed by near ultra-violet light (without visible light). 4. Inhibition in ultra-violet light is not due to stimulation (by the ultra-violet light) of the animal to luminesce, thereby using up the store of photogenic material. 5. Animals stimulated mechanically several times and placed in ultra-violet light show a luminescence along the meridians in the same positions as the luminescence that appears on stimulation. This luminescence in the ultra-violet or "tonic luminescence," is not obtained with light adapted ctenophores and is interpreted to be a fluorescence of the product of oxidation of the photogenic material. 6. Marked fluorescence of the luminous organ of the glowworm (Photuris) and of the luminous slime of Chatopterus may be observed in ultra-violet but no marked fluorescence of the luminous substances of Cypridina is apparent. 7. Evidence is accumulating to show a close relation between fluorescent and chemiluminescent substances in animals, similar to that described for unsaturated silicon compounds and the Grignard reagents.

Spectral calibration of the fluorescence telescopes of the Pierre Auger Observatory

NASA Astrophysics Data System (ADS)

Aab, A.; Abreu, P.; Aglietta, M.; Al Samarai, I.; Albuquerque, I. F. M.; Allekotte, I.; Almela, A.; Alvarez Castillo, J.; Alvarez-Muñiz, J.; Anastasi, G. A.; Anchordoqui, L.; Andrada, B.; Andringa, S.; Aramo, C.; Arqueros, F.; Arsene, N.; Asorey, H.; Assis, P.; Aublin, J.; Avila, G.; Badescu, A. M.; Balaceanu, A.; Barbato, F.; Barreira Luz, R. J.; Beatty, J. J.; Becker, K. H.; Bellido, J. A.; Berat, C.; Bertaina, M. E.; Biermann, P. L.; Biteau, J.; Blaess, S. G.; Blanco, A.; Blazek, J.; Bleve, C.; Boháčová, M.; Boncioli, D.; Bonifazi, C.; Borodai, N.; Botti, A. M.; Brack, J.; Brancus, I.; Bretz, T.; Bridgeman, A.; Briechle, F. L.; Buchholz, P.; Bueno, A.; Buitink, S.; Buscemi, M.; Caballero-Mora, K. S.; Caccianiga, L.; Cancio, A.; Canfora, F.; Caramete, L.; Caruso, R.; Castellina, A.; Catalani, F.; Cataldi, G.; Cazon, L.; Chavez, A. G.; Chinellato, J. A.; Chudoba, J.; Clay, R. W.; Cobos, A.; Colalillo, R.; Coleman, A.; Collica, L.; Coluccia, M. R.; Conceição, R.; Consolati, G.; Contreras, F.; Cooper, M. J.; Coutu, S.; Covault, C. E.; Cronin, J.; D'Amico, S.; Daniel, B.; Dasso, S.; Daumiller, K.; Dawson, B. R.; de Almeida, R. M.; de Jong, S. J.; De Mauro, G.; de Mello Neto, J. R. T.; De Mitri, I.; de Oliveira, J.; de Souza, V.; Debatin, J.; Deligny, O.; Díaz Castro, M. L.; Diogo, F.; Dobrigkeit, C.; D'Olivo, J. C.; Dorosti, Q.; dos Anjos, R. C.; Dova, M. T.; Dundovic, A.; Ebr, J.; Engel, R.; Erdmann, M.; Erfani, M.; Escobar, C. O.; Espadanal, J.; Etchegoyen, A.; Falcke, H.; Farmer, J.; Farrar, G.; Fauth, A. C.; Fazzini, N.; Fenu, F.; Fick, B.; Figueira, J. M.; Filipčič, A.; Fratu, O.; Freire, M. M.; Fujii, T.; Fuster, A.; Gaior, R.; García, B.; Garcia-Pinto, D.; Gaté, F.; Gemmeke, H.; Gherghel-Lascu, A.; Giaccari, U.; Giammarchi, M.; Giller, M.; Głas, D.; Glaser, C.; Golup, G.; Gómez Berisso, M.; Gómez Vitale, P. F.; González, N.; Gookin, B.; Gorgi, A.; Gorham, P.; Grillo, A. F.; Grubb, T. D.; Guarino, F.; Guedes, G. P.; Halliday, R.; Hampel, M. R.; Hansen, P.; Harari, D.; Harrison, T. A.; Harton, J. L.; Haungs, A.; Hebbeker, T.; Heck, D.; Heimann, P.; Herve, A. E.; Hill, G. C.; Hojvat, C.; Holt, E.; Homola, P.; Hörandel, J. R.; Horvath, P.; Hrabovský, M.; Huege, T.; Hulsman, J.; Insolia, A.; Isar, P. G.; Jandt, I.; Johnsen, J. A.; Josebachuili, M.; Jurysek, J.; Kääpä, A.; Kambeitz, O.; Kampert, K. H.; Keilhauer, B.; Kemmerich, N.; Kemp, E.; Kemp, J.; Kieckhafer, R. M.; Klages, H. O.; Kleifges, M.; Kleinfeller, J.; Krause, R.; Krohm, N.; Kuempel, D.; Kukec Mezek, G.; Kunka, N.; Kuotb Awad, A.; Lago, B. L.; LaHurd, D.; Lang, R. G.; Lauscher, M.; Legumina, R.; Leigui de Oliveira, M. A.; Letessier-Selvon, A.; Lhenry-Yvon, I.; Link, K.; Lo Presti, D.; Lopes, L.; López, R.; López Casado, A.; Lorek, R.; Luce, Q.; Lucero, A.; Malacari, M.; Mallamaci, M.; Mandat, D.; Mantsch, P.; Mariazzi, A. G.; Mariş, I. C.; Marsella, G.; Martello, D.; Martinez, H.; Martínez Bravo, O.; Masías Meza, J. J.; Mathes, H. J.; Mathys, S.; Matthews, J.; Matthews, J. A. J.; Matthiae, G.; Mayotte, E.; Mazur, P. O.; Medina, C.; Medina-Tanco, G.; Melo, D.; Menshikov, A.; Merenda, K.-D.; Michal, S.; Micheletti, M. I.; Middendorf, L.; Miramonti, L.; Mitrica, B.; Mockler, D.; Mollerach, S.; Montanet, F.; Morello, C.; Mostafá, M.; Müller, A. L.; Müller, G.; Muller, M. A.; Müller, S.; Mussa, R.; Naranjo, I.; Nellen, L.; Nguyen, P. H.; Niculescu-Oglinzanu, M.; Niechciol, M.; Niemietz, L.; Niggemann, T.; Nitz, D.; Nosek, D.; Novotny, V.; Nožka, L.; Núñez, L. A.; Ochilo, L.; Oikonomou, F.; Olinto, A.; Palatka, M.; Pallotta, J.; Papenbreer, P.; Parente, G.; Parra, A.; Paul, T.; Pech, M.; Pedreira, F.; Pȩkala, J.; Pelayo, R.; Peña-Rodriguez, J.; Pereira, L. A. S.; Perlin, M.; Perrone, L.; Peters, C.; Petrera, S.; Phuntsok, J.; Piegaia, R.; Pierog, T.; Pimenta, M.; Pirronello, V.; Platino, M.; Plum, M.; Porowski, C.; Prado, R. R.; Privitera, P.; Prouza, M.; Quel, E. J.; Querchfeld, S.; Quinn, S.; Ramos-Pollan, R.; Rautenberg, J.; Ravignani, D.; Ridky, J.; Riehn, F.; Risse, M.; Ristori, P.; Rizi, V.; Rodrigues de Carvalho, W.; Rodriguez Fernandez, G.; Rodriguez Rojo, J.; Rogozin, D.; Roncoroni, M. J.; Roth, M.; Roulet, E.; Rovero, A. C.; Ruehl, P.; Saffi, S. J.; Saftoiu, A.; Salamida, F.; Salazar, H.; Saleh, A.; Salesa Greus, F.; Salina, G.; Sánchez, F.; Sanchez-Lucas, P.; Santos, E. M.; Santos, E.; Sarazin, F.; Sarmento, R.; Sarmiento-Cano, C.; Sato, R.; Schauer, M.; Scherini, V.; Schieler, H.; Schimp, M.; Schmidt, D.; Scholten, O.; Schovánek, P.; Schröder, F. G.; Schröder, S.; Schulz, A.; Schumacher, J.; Sciutto, S. J.; Segreto, A.; Shadkam, A.; Shellard, R. C.; Sigl, G.; Silli, G.; Sima, O.; Śmiałkowski, A.; Šmída, R.; Snow, G. R.; Sommers, P.; Sonntag, S.; Squartini, R.; Stanca, D.; Stanič, S.; Stasielak, J.; Stassi, P.; Stolpovskiy, M.; Strafella, F.; Streich, A.; Suarez, F.; Suarez Durán, M.; Sudholz, T.; Suomijärvi, T.; Supanitsky, A. D.; Šupík, J.; Swain, J.; Szadkowski, Z.; Taboada, A.; Taborda, O. A.; Theodoro, V. M.; Timmermans, C.; Todero Peixoto, C. J.; Tomankova, L.; Tomé, B.; Torralba Elipe, G.; Travnicek, P.; Trini, M.; Ulrich, R.; Unger, M.; Urban, M.; Valdés Galicia, J. F.; Valiño, I.; Valore, L.; van Aar, G.; van Bodegom, P.; van den Berg, A. M.; van Vliet, A.; Varela, E.; Vargas Cárdenas, B.; Varner, G.; Vázquez, R. A.; Veberič, D.; Ventura, C.; Vergara Quispe, I. D.; Verzi, V.; Vicha, J.; Villaseñor, L.; Vorobiov, S.; Wahlberg, H.; Wainberg, O.; Walz, D.; Watson, A. A.; Weber, M.; Weindl, A.; Wiencke, L.; Wilczyński, H.; Wirtz, M.; Wittkowski, D.; Wundheiler, B.; Yang, L.; Yushkov, A.; Zas, E.; Zavrtanik, D.; Zavrtanik, M.; Zepeda, A.; Zimmermann, B.; Ziolkowski, M.; Zong, Z.; Zuccarello, F.; Pierre Auger Collaboration

2017-10-01

We present a novel method to measure precisely the relative spectral response of the fluorescence telescopes of the Pierre Auger Observatory. We used a portable light source based on a xenon flasher and a monochromator to measure the relative spectral efficiencies of eight telescopes in steps of 5 nm from 280 nm to 440 nm. Each point in a scan had approximately 2 nm FWHM out of the monochromator. Different sets of telescopes in the observatory have different optical components, and the eight telescopes measured represent two each of the four combinations of components represented in the observatory. We made an end-to-end measurement of the response from different combinations of optical components, and the monochromator setup allowed for more precise and complete measurements than our previous multi-wavelength calibrations. We find an overall uncertainty in the calibration of the spectral response of most of the telescopes of 1.5% for all wavelengths; the six oldest telescopes have larger overall uncertainties of about 2.2%. We also report changes in physics measurables due to the change in calibration, which are generally small.

Multi-photon excited luminescence of magnetic FePt core-shell nanoparticles.

PubMed

Seemann, K M; Kuhn, B

2014-07-01

We present magnetic FePt nanoparticles with a hydrophilic, inert, and biocompatible silico-tungsten oxide shell. The particles can be functionalized, optically detected, and optically manipulated. To show the functionalization the fluorescent dye NOPS was bound to the FePt core-shell nanoparticles with propyl-triethoxy-silane linkers and fluorescence of the labeled particles were observed in ethanol (EtOH). In aqueous dispersion the NOPS fluorescence is quenched making them invisible using 1-photon excitation. However, we observe bright luminescence of labeled and even unlabeled magnetic core-shell nanoparticles with multi-photon excitation. Luminescence can be detected in the near ultraviolet and the full visible spectral range by near infrared multi-photon excitation. For optical manipulation, we were able to drag clusters of particles, and maybe also single particles, by a focused laser beam that acts as optical tweezers by inducing an electric dipole in the insulated metal nanoparticles. In a first application, we show that the luminescence of the core-shell nanoparticles is bright enough for in vivo multi-photon imaging in the mouse neocortex down to cortical layer 5.

Hyperspectral Fluorescence and Reflectance Imaging Instrument

NASA Technical Reports Server (NTRS)

Ryan, Robert E.; O'Neal, S. Duane; Lanoue, Mark; Russell, Jeffrey

2008-01-01

The system is a single hyperspectral imaging instrument that has the unique capability to acquire both fluorescence and reflectance high-spatial-resolution data that is inherently spatially and spectrally registered. Potential uses of this instrument include plant stress monitoring, counterfeit document detection, biomedical imaging, forensic imaging, and general materials identification. Until now, reflectance and fluorescence spectral imaging have been performed by separate instruments. Neither a reflectance spectral image nor a fluorescence spectral image alone yields as much information about a target surface as does a combination of the two modalities. Before this system was developed, to benefit from this combination, analysts needed to perform time-consuming post-processing efforts to co-register the reflective and fluorescence information. With this instrument, the inherent spatial and spectral registration of the reflectance and fluorescence images minimizes the need for this post-processing step. The main challenge for this technology is to detect the fluorescence signal in the presence of a much stronger reflectance signal. To meet this challenge, the instrument modulates artificial light sources from ultraviolet through the visible to the near-infrared part of the spectrum; in this way, both the reflective and fluorescence signals can be measured through differencing processes to optimize fluorescence and reflectance spectra as needed. The main functional components of the instrument are a hyperspectral imager, an illumination system, and an image-plane scanner. The hyperspectral imager is a one-dimensional (line) imaging spectrometer that includes a spectrally dispersive element and a two-dimensional focal plane detector array. The spectral range of the current imaging spectrometer is between 400 to 1,000 nm, and the wavelength resolution is approximately 3 nm. The illumination system consists of narrowband blue, ultraviolet, and other discrete

Augmentation of the IUE Ultraviolet Spectral Atlas

NASA Astrophysics Data System (ADS)

Wu, Chi-Chao

Most likely IUE is the only and last satellite which will support a survey program to record the ultraviolet spectrum of a large number of bright normal stars. It is important to have a library of high quality Low dispersion spectra of sufficient number of stars that provide good coverage in spectral type and luminosity class. Such a library is invaluable for stellar population synthesis of galaxies, studying the nature of distant galaxies, establishing a UV spectral classification system, providing comparison stars for interstellar extinction studies and for peculiar objects or binary systems, studying the effects of temperature, gravity and metallicity on stellar UV spectra, and as a teaching aid. We propose to continue observations of normal stars in order to provide (1) a more complete coverage of the spectral type and luminosity class, and (2) more than one star per spectral typeluminosity class combination to guard against variability and peculiarity, and to allow a finite range of temperature, gravity, and metallicity in a given combination. Our primary goal is to collect the data and make them available to the community immediately (without claiming the 6-month proprietary right). The data will be published in the IUE Newsletter as soon as practical, and the data will be prepared for distribution by the IUE Observatory and the NSSDC.

A six-color four-laser mobile platform for multi-spectral fluorescence imaging endoscopy

NASA Astrophysics Data System (ADS)

Black, John F.; Tate, Tyler; Keenan, Molly; Swan, Elizabeth; Utzinger, Urs; Barton, Jennifer

2015-03-01

The properties of multi-spectral fluorescence imaging using deep-UV-illumination have recently been explored using a fiber-coupled thermal source at 280 nm. The resulting images show a remarkable level of contrast thought to result from the signal being overwhelmingly generated in the uppermost few cell layers of tissue, making this approach valuable for the study of diseases that originate in the endothelial tissues of the body. With a view to extending the technique with new wavelengths, and improving beam quality for efficient small core fiber coupling we have developed a mobile self-contained tunable solid-state laser source of deep UV light. An alexandrite laser, lasing at around 750 nm is frequency doubled to produce 375 nm and then tripled to produce 250 nm light. An optical deck added to the system allows other laser sources to be incorporated into the UV beam-line and a lens system has been designed to couple these sources into a single delivery fiber with core diameters down to 50 microns. Our system incorporates five wavelengths [250 nm, 375 nm, 442 nm (HeCd), 543 nm (HeNe) and 638 nm (diode laser)] as the illumination source for a small diameter falloposcope designed for the study of the distal Fallopian tube origins of high grade serous ovarian cancer. The tunability of alexandrite offers the potential to generate other wavelengths in the 720-800, 360-400 and 240-265 nm ranges, plus other non-linear optical conversion techniques taking advantage of the high peak powers of the laser.

Very High Spectral Resolution Imaging Spectroscopy: the Fluorescence Explorer (FLEX) Mission

NASA Technical Reports Server (NTRS)

Moreno, Jose F.; Goulas, Yves; Huth, Andreas; Middleton, Elizabeth; Miglietta, Franco; Mohammed, Gina; Nedbal, Ladislav; Rascher, Uwe; Verhoef, Wouter; Drusch, Matthias

2016-01-01

The Fluorescence Explorer (FLEX) mission has been recently selected as the 8th Earth Explorer by the European Space Agency (ESA). It will be the first mission specifically designed to measure from space vegetation fluorescence emission, by making use of very high spectral resolution imaging spectroscopy techniques. Vegetation fluorescence is the best proxy to actual vegetation photosynthesis which can be measurable from space, allowing an improved quantification of vegetation carbon assimilation and vegetation stress conditions, thus having key relevance for global mapping of ecosystems dynamics and aspects related with agricultural production and food security. The FLEX mission carries the FLORIS spectrometer, with a spectral resolution in the range of 0.3 nm, and is designed to fly in tandem with Copernicus Sentinel-3, in order to provide all the necessary spectral / angular information to disentangle emitted fluorescence from reflected radiance, and to allow proper interpretation of the observed fluorescence spatial and temporal dynamics.

Fluorescence Intrinsic Characterization of Excitation-Emission Matrix Using Multi-Dimensional Ensemble Empirical Mode Decomposition

PubMed Central

Chang, Chi-Ying; Chang, Chia-Chi; Hsiao, Tzu-Chien

2013-01-01

Excitation-emission matrix (EEM) fluorescence spectroscopy is a noninvasive method for tissue diagnosis and has become important in clinical use. However, the intrinsic characterization of EEM fluorescence remains unclear. Photobleaching and the complexity of the chemical compounds make it difficult to distinguish individual compounds due to overlapping features. Conventional studies use principal component analysis (PCA) for EEM fluorescence analysis, and the relationship between the EEM features extracted by PCA and diseases has been examined. The spectral features of different tissue constituents are not fully separable or clearly defined. Recently, a non-stationary method called multi-dimensional ensemble empirical mode decomposition (MEEMD) was introduced; this method can extract the intrinsic oscillations on multiple spatial scales without loss of information. The aim of this study was to propose a fluorescence spectroscopy system for EEM measurements and to describe a method for extracting the intrinsic characteristics of EEM by MEEMD. The results indicate that, although PCA provides the principal factor for the spectral features associated with chemical compounds, MEEMD can provide additional intrinsic features with more reliable mapping of the chemical compounds. MEEMD has the potential to extract intrinsic fluorescence features and improve the detection of biochemical changes. PMID:24240806

Spatial variability of oceanic phycoerythrin spectral types derived from airborne laser-induced fluorescence emissions

NASA Astrophysics Data System (ADS)

Hoge, Frank E.; Wright, C. Wayne; Kana, Todd M.; Swift, Robert N.; Yungel, James K.

1998-07-01

We report spatial variability of oceanic phycoerythrin spectral types detected by means of a blue spectral shift in airborne laser-induced fluorescence emission. The blue shift of the phycoerythrobilin fluorescence is known from laboratory studies to be induced by phycourobilin chromophore substitution at phycoerythrobilin chromophore sites in some strains of phycoerythrin-containing marine cyanobacteria. The airborne 532-nm laser-induced phycoerythrin fluorescence of the upper oceanic volume showed distinct segregation of cyanobacterial chromophore types in a flight transect from coastal water to the Sargasso Sea in the western North Atlantic. High phycourobilin levels were restricted to the oceanic (oligotrophic) end of the flight transect, in agreement with historical ship findings. These remotely observed phycoerythrin spectral fluorescence shifts have the potential to permit rapid, wide-area studies of the spatial variability of spectrally distinct cyanobacteria, especially across interfacial regions of coastal and oceanic water masses. Airborne laser-induced phytoplankton spectral fluorescence observations also further the development of satellite algorithms for passive detection of phytoplankton pigments. Optical modifications to the NASA Airborne Oceanographic Lidar are briefly described that permitted observation of the fluorescence spectral shifts.

Reduction of short wavelength reflectance of multi-wall carbon nanotubes through ultraviolet laser irradiation

NASA Astrophysics Data System (ADS)

Stephens, Michelle S.; Simonds, Brian J.; Yung, Christopher S.; Conklin, Davis; Livigni, David J.; Oliva, Alberto Remesal; Lehman, John H.

2018-05-01

Multi-wall carbon nanotube coatings are used as broadband, low-reflectance absorbers for bolometric applications and for stray light control. They are also used as high emittance blackbody radiators. Irradiation of single wall carbon nanotubes with ultraviolet (UV) laser light has been shown to remove amorphous carbon debris, but there have been few investigations of the interaction of UV light with the more complex physics of multi-wall carbon nanotubes. We present measurements of reflectance and surface morphology before and after exposure of multi-wall carbon nanotube coatings to 248 nm UV laser light. We show that UV exposure reduces the reflectivity at wavelengths below 600 nm and present modeling of the thermal cycling the UV exposure causes at the surface of the carbon nanotubes. This effect can be used to flatten the spectral shape of the reflectivity curve of carbon nanotube absorber coatings used for broadband applications. Finally, we find that the effect of UV exposure depends on the nanotube growth process.

Spectral line discriminator for passive detection of fluorescence

NASA Technical Reports Server (NTRS)

Kebabian, Paul L. (Inventor)

1996-01-01

A method and apparatus for detecting fluorescence from sunlit plants is based on spectral line discrimination using the A-band and B-band absorption of atmospheric oxygen. Light from a plant including scattered sunlight and the fluorescence from chlorophyll is passed through a chopper into a cell containing low-pressure, high-purity oxygen. A-band or B-band wavelengths present in the light are absorbed by the oxygen in the cell. When the chopper is closed, the absorbed light is remitted as fluorescence into a detector. The intensity of the fluorescence from the oxygen is proportional to the intensity of fluorescence from the plant.

Analysis of multi-channel microscopy: Spectral self-interference, multi-detector confocal and 4Pi systems

NASA Astrophysics Data System (ADS)

Davis, Brynmor J.

Fluorescence microscopy is an important and ubiquitous tool in biological imaging due to the high specificity with which fluorescent molecules can be attached to an organism and the subsequent nondestructive in-vivo imaging allowed. Focused-light microscopies allow three-dimensional fluorescence imaging but their resolution is restricted by diffraction. This effect is particularly limiting in the axial dimension as the diffraction-limited focal volume produced by a lens is more extensive along the optical axis than perpendicular to it. Approaches such as confocal microscopy and 4Pi microscopy have been developed to improve the axial resolution. Spectral Self-Interference Fluorescence Microscopy (SSFM) is another high-axial-resolution technique and is the principal subject of this dissertation. Nanometer-precision localization of a single fluorescent layer has been demonstrated using SSFM. This accuracy compares favorably with the axial resolutions given by confocal and 4Pi systems at similar operating parameters (these resolutions are approximately 350nm and 80nm respectively). This theoretical work analyzes the expected performance of the SSFM system when imaging a general object, i.e. an arbitrary fluorophore density function rather than a single layer. An existing model of SSFM is used in simulations to characterize the system's resolution. Several statistically-based reconstruction methods are applied to show that the expected resolution for SSFM is similar to 4Pi microscopy for a general object but does give very high localization accuracy when the object is known to consist of a limited number of layers. SSFM is then analyzed in a linear systems framework and shown to have strong connections, both physically and mathematically, to a multi-channel 4Pi microscope. Fourier-domain analysis confirms that SSFM cannot be expected to outperform this multi-channel 4Pi instrument. Differences between the channels in spatial-scanning, multi-channel microscopies are then

Traveling reference spectroradiometer for routine quality assurance of spectral solar ultraviolet irradiance measurements.

PubMed

Gröbner, Julian; Schreder, Josef; Kazadzis, Stelios; Bais, Alkiviadis F; Blumthaler, Mario; Görts, Peter; Tax, Rick; Koskela, Tapani; Seckmeyer, Gunther; Webb, Ann R; Rembges, Diana

2005-09-01

A transportable reference spectroradiometer for measuring spectral solar ultraviolet irradiance has been developed and validated. The expanded uncertainty of solar irradiance measurements with this reference spectroradiometer, based on the described methodology, is 8.8% to 4.6%, depending on the wavelength and the solar zenith angle. The accuracy of the spectroradiometer was validated by repeated site visits to two European UV monitoring sites as well as by regular comparisons with the reference spectroradiometer of the European Reference Centre for UV radiation measurements in Ispra, Italy. The spectral solar irradiance measurements of the Quality Assurance of Spectral Ultraviolet Measurements in Europe through the Development of a Transportable Unit (QASUME) spectroradiometer and these three spectroradiometers have agreed to better than 6% during the ten intercomparison campaigns held from 2002 to 2004. If the differences in irradiance scales of as much as 2% are taken into account, the agreement is of the order of 4% over the wavelength range of 300-400 nm.

Fluorescence hyperspectral imaging (fHSI) using a spectrally resolved detector array

PubMed Central

Luthman, Anna Siri; Dumitru, Sebastian; Quiros‐Gonzalez, Isabel; Joseph, James

2017-01-01

Abstract The ability to resolve multiple fluorescent emissions from different biological targets in video rate applications, such as endoscopy and intraoperative imaging, has traditionally been limited by the use of filter‐based imaging systems. Hyperspectral imaging (HSI) facilitates the detection of both spatial and spectral information in a single data acquisition, however, instrumentation for HSI is typically complex, bulky and expensive. We sought to overcome these limitations using a novel robust and low cost HSI camera based on a spectrally resolved detector array (SRDA). We integrated this HSI camera into a wide‐field reflectance‐based imaging system operating in the near‐infrared range to assess the suitability for in vivo imaging of exogenous fluorescent contrast agents. Using this fluorescence HSI (fHSI) system, we were able to accurately resolve the presence and concentration of at least 7 fluorescent dyes in solution. We also demonstrate high spectral unmixing precision, signal linearity with dye concentration and at depth in tissue mimicking phantoms, and delineate 4 fluorescent dyes in vivo. Our approach, including statistical background removal, could be directly generalised to broader spectral ranges, for example, to resolve tissue reflectance or autofluorescence and in future be tailored to video rate applications requiring snapshot HSI data acquisition. PMID:28485130

Optimal fluorescence waveband determination for detecting defect cherry tomatoes using fluorescence excitation-emission matrix

USDA-ARS?s Scientific Manuscript database

A multi-spectral fluorescence imaging technique was used to detect defect cherry tomatoes. The fluorescence excitation and emission matrix was used to measure for defects, sound surface, and stem areas to determine the optimal fluorescence excitation and emission wavelengths for discrimination. Two-...

Spectral calibration of the fluorescence telescopes of the Pierre Auger Observatory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Aab, A.; Abreu, P.; Aglietta, M.

We present a novel method to measure precisely the relative spectral response of the fluorescence telescopes of the Pierre Auger Observatory. Here, we used a portable light source based on a xenon flasher and a monochromator to measure the relative spectral efficiencies of eight telescopes in steps of 5 nm from 280 nm to 440 nm. Each point in a scan had approximately 2 nm FWHM out of the monochromator. Different sets of telescopes in the observatory have different optical components, and the eight telescopes measured represent two each of the four combinations of components represented in the observatory. Wemore » made an end-to-end measurement of the response from different combinations of optical components, and the monochromator setup allowed for more precise and complete measurements than our previous multi-wavelength calibrations. We find an overall uncertainty in the calibration of the spectral response of most of the telescopes of 1.5% for all wavelengths; the six oldest telescopes have larger overall uncertainties of about 2.2%. We also report changes in physics measureables due to the change in calibration, which are generally small.« less

Spectral calibration of the fluorescence telescopes of the Pierre Auger Observatory

DOE PAGES

Aab, A.; Abreu, P.; Aglietta, M.; ...

2017-09-08

We present a novel method to measure precisely the relative spectral response of the fluorescence telescopes of the Pierre Auger Observatory. Here, we used a portable light source based on a xenon flasher and a monochromator to measure the relative spectral efficiencies of eight telescopes in steps of 5 nm from 280 nm to 440 nm. Each point in a scan had approximately 2 nm FWHM out of the monochromator. Different sets of telescopes in the observatory have different optical components, and the eight telescopes measured represent two each of the four combinations of components represented in the observatory. Wemore » made an end-to-end measurement of the response from different combinations of optical components, and the monochromator setup allowed for more precise and complete measurements than our previous multi-wavelength calibrations. We find an overall uncertainty in the calibration of the spectral response of most of the telescopes of 1.5% for all wavelengths; the six oldest telescopes have larger overall uncertainties of about 2.2%. We also report changes in physics measureables due to the change in calibration, which are generally small.« less

Double-excitation fluorescence spectral imaging: eliminating tissue auto-fluorescence from in vivo PPIX measurements

NASA Astrophysics Data System (ADS)

Torosean, Sason; Flynn, Brendan; Samkoe, Kimberley S.; Davis, Scott C.; Gunn, Jason; Axelsson, Johan; Pogue, Brian W.

2012-02-01

An ultrasound coupled handheld-probe-based optical fluorescence molecular tomography (FMT) system has been in development for the purpose of quantifying the production of Protoporphyrin IX (PPIX) in aminolevulinic acid treated (ALA), Basal Cell Carcinoma (BCC) in vivo. The design couples fiber-based spectral sampling of PPIX fluorescence emission with a high frequency ultrasound imaging system, allowing regionally localized fluorescence intensities to be quantified [1]. The optical data are obtained by sequential excitation of the tissue with a 633nm laser, at four source locations and five parallel detections at each of the five interspersed detection locations. This method of acquisition permits fluorescence detection for both superficial and deep locations in ultrasound field. The optical boundary data, tissue layers segmented from ultrasound image and diffusion theory are used to estimate the fluorescence in tissue layers. To improve the recovery of the fluorescence signal of PPIX, eliminating tissue autofluorescence is of great importance. Here the approach was to utilize measurements which straddled the steep Qband excitation peak of PPIX, via the integration of an additional laser source, exciting at 637 nm; a wavelength with a 2 fold lower PPIX excitation value than 633nm.The auto-fluorescence spectrum acquired from the 637 nm laser is then used to spectrally decouple the fluorescence data and produce an accurate fluorescence emission signal, because the two wavelengths have very similar auto-fluorescence but substantially different PPIX excitation levels. The accuracy of this method, using a single source detector pair setup, is verified through animal tumor model experiments, and the result is compared to different methods of fluorescence signal recovery.

Spectral Demultiplexing in Holographic and Fluorescent On-chip Microscopy

NASA Astrophysics Data System (ADS)

Sencan, Ikbal; Coskun, Ahmet F.; Sikora, Uzair; Ozcan, Aydogan

2014-01-01

Lensfree on-chip imaging and sensing platforms provide compact and cost-effective designs for various telemedicine and lab-on-a-chip applications. In this work, we demonstrate computational solutions for some of the challenges associated with (i) the use of broadband, partially-coherent illumination sources for on-chip holographic imaging, and (ii) multicolor detection for lensfree fluorescent on-chip microscopy. Specifically, we introduce spectral demultiplexing approaches that aim to digitally narrow the spectral content of broadband illumination sources (such as wide-band light emitting diodes or even sunlight) to improve spatial resolution in holographic on-chip microscopy. We also demonstrate the application of such spectral demultiplexing approaches for wide-field imaging of multicolor fluorescent objects on a chip. These computational approaches can be used to replace e.g., thin-film interference filters, gratings or other optical components used for spectral multiplexing/demultiplexing, which can form a desirable solution for cost-effective and compact wide-field microscopy and sensing needs on a chip.

Ultraviolet spectral reflectance of carbonaceous materials

NASA Astrophysics Data System (ADS)

Applin, Daniel M.; Izawa, Matthew R. M.; Cloutis, Edward A.; Gillis-Davis, Jeffrey J.; Pitman, Karly M.; Roush, Ted L.; Hendrix, Amanda R.; Lucey, Paul G.

2018-06-01

A number of planetary spacecraft missions have carried instruments with sensors covering the ultraviolet (UV) wavelength range. However, there exists a general lack of relevant UV reflectance laboratory data to compare against these planetary surface remote sensing observations in order to make confident material identifications. To address this need, we have systematically analyzed reflectance spectra of carbonaceous materials in the 200-500 nm spectral range, and found spectral-compositional-structural relationships that suggest this wavelength region could distinguish between otherwise difficult-to-identify carbon phases. In particular (and by analogy with the infrared spectral region), large changes over short wavelength intervals in the refractive indices associated with the trigonal sp2π-π* transition of carbon can lead to Fresnel peaks and Christiansen-like features in reflectance. Previous studies extending to shorter wavelengths also show that anomalous dispersion caused by the σ-σ* transition associated with both the trigonal sp2 and tetrahedral sp3 sites causes these features below λ = 200 nm. The peak wavelength positions and shapes of π-π* and σ-σ* features contain information on sp3/sp2, structure, crystallinity, and powder grain size. A brief comparison with existing observational data indicates that the carbon fraction of the surface of Mercury is likely amorphous and submicroscopic, as is that on the surface of the martian satellites Phobos and Deimos, and possibly comet 67P/Churyumov-Gerasimenko, while further coordinated observations and laboratory experiments should refine these feature assignments and compositional hypotheses. The new laboratory diffuse reflectance data reported here provide an important new resource for interpreting UV reflectance measurements from planetary surfaces throughout the solar system, and confirm that the UV can be rich in important spectral information.

Robust demarcation of basal cell carcinoma by dependent component analysis-based segmentation of multi-spectral fluorescence images.

PubMed

Kopriva, Ivica; Persin, Antun; Puizina-Ivić, Neira; Mirić, Lina

2010-07-02

This study was designed to demonstrate robust performance of the novel dependent component analysis (DCA)-based approach to demarcation of the basal cell carcinoma (BCC) through unsupervised decomposition of the red-green-blue (RGB) fluorescent image of the BCC. Robustness to intensity fluctuation is due to the scale invariance property of DCA algorithms, which exploit spectral and spatial diversities between the BCC and the surrounding tissue. Used filtering-based DCA approach represents an extension of the independent component analysis (ICA) and is necessary in order to account for statistical dependence that is induced by spectral similarity between the BCC and surrounding tissue. This generates weak edges what represents a challenge for other segmentation methods as well. By comparative performance analysis with state-of-the-art image segmentation methods such as active contours (level set), K-means clustering, non-negative matrix factorization, ICA and ratio imaging we experimentally demonstrate good performance of DCA-based BCC demarcation in two demanding scenarios where intensity of the fluorescent image has been varied almost two orders of magnitude. Copyright 2010 Elsevier B.V. All rights reserved.

Plasmonic enhancement of ultraviolet fluorescence

NASA Astrophysics Data System (ADS)

Jiao, Xiaojin

Plasmonics relates to the interaction between electromagnetic radiation and conduction electrons at metallic interfaces or in metallic nanostructures. Surface plasmons are collective electron oscillations at a metal surface, which can be manipulated by shape, texture and material composition. Plasmonic applications cover a broad spectrum from visible to near infrared, including biosensing, nanolithography, spectroscopy, optoelectronics, photovoltaics and so on. However, there remains a gap in this activity in the ultraviolet (UV, < 400 nm), where significant opportunity exists for both fundamental and application research. Motivating factors in the study of UV Plasmonics are the direct access to biomolecular resonances and native fluorescence, resonant Raman scattering interactions, and the potential for exerting control over photochemical reactions. This dissertation aims to fill in the gap of Plasmonics in the UV with efforts of design, fabrication and characterization of aluminium (Al) and magnesium (Mg) nanostructures for the application of label-free bimolecular detection via native UV fluorescence. The first contribution of this dissertation addresses the design of Al nanostructures in the context of UV fluorescence enhancement. A design method that combines analytical analysis with numerical simulation has been developed. Performance of three canonical plasmonic structures---the dipole antenna, bullseye nanoaperture and nanoaperture array---has been compared. The optimal geometrical parameters have been determined. A novel design of a compound bullseye structure has been proposed and numerically analyzed for the purpose of compensating for the large Stokes shift typical of UV fluorescence. Second, UV lifetime modification of diffusing molecules by Al nanoapertures has been experimentally demonstrated for the first time. Lifetime reductions of ~3.5x have been observed for the high quantum yield (QY) laser dye p-terphenyl in a 60 nm diameter aperture with 50

Relationship between anaerobic digestion of biodegradable solid waste and spectral characteristics of the derived liquid digestate.

PubMed

Zheng, Wei; Lü, Fan; Phoungthong, Khamphe; He, Pinjing

2014-06-01

The evolution of spectral properties during anaerobic digestion (AD) of 29 types of biodegradable solid waste was investigated to determine if spectral characteristics could be used for assessment of biological stabilization during AD. Biochemical methane potential tests were conducted and spectral indicators (including the ratio of ultraviolet-visible absorbance at 254nm to dissolved organic carbon concentration (SUVA254), the ratio of ultraviolet-visible absorbance measured at 465nm and 665nm (E4/E6), and the abundance of fluorescence peaks) were measured at different AD phases. Inter-relationship between organic degradation and spectral indicators were analyzed by principle component analysis. The results shows that from methane production phase to the end of methane production phase, SUVA254 increased by 0.16-10.93 times, the abundance of fulvic acid-like compounds fluorescence peak increased by 0.01-0.54 times, the abundance of tyrosine fluorescence peak decreased by 0.03-0.64 times. Therefore, these indicators were useful to judge the course of mixed waste digestion. Copyright © 2014 Elsevier Ltd. All rights reserved.

Augmentation of the IUE Ultraviolet Spectral Atlas

NASA Astrophysics Data System (ADS)

Wu, Chi-Chao

IUE is the only and last satellite which will support a survey program to record the ultraviolet spectrum of a large number of bright normal stars. It is important to have a library of high quality low dispersion spectra of sufficient number of stars that provide good coverage in spectral type and luminosity class. Such a library is invaluable for stellar population synthesis of galaxies, studying the nature of distant galaxies, establishing a UV spectral classification system, providing comparison stars for interstellar extinction studies and for peculiar objects or binary systems, studying the effects of temperature, gravity and metallicity on stellar UV spectra, and as a teaching aid. We propose to continue observations of normal stars in order to provide (1) a stellar library as complete as practical, which will be able to support astronomical research by the scientific community long into the future, and (2) a sufficient sample of stars to guard against variability and peculiarity, and to allow a finite range of temperature, gravity, and metallicity in a given spectral type-luminosity class combination. Our primary goal is to collect the data and make them available to the community immediately (without claiming the 6-month proprietary right). The data will be published in the IUE Newsletter as soon as practical, and the data will be prepared for distribution by the IUE Observatory and the NSSDC.

An improved ultraviolet spectral line list for the symbiotic star RR Telescopii

NASA Technical Reports Server (NTRS)

Doschek, G. A.; Feibelman, W. A.

1993-01-01

We have remeasured wavelengths and intensities of International Ultraviolet Explorer (IUE) spectra of the symbiotic star, RR Tel. The main work is centered on the long 820 minute exposure high-resolution spectrum obtained on 1983 June 18. The list is intended to serve as a source of improved intensities and wavelengths for the ultraviolet spectrum of this star. A complete line list with intensities based on this exposure has not been published previously. The strongest spectral lines are saturated in the 820 minute exposure, and intensities for these lines are mostly obtained from a 20 minute exposure obtained on the same day. A few intensities are obtained from other exposures if neither the 820 nor the 20 minute exposure is satisfactory. There are 111 lines in our list between 1168 and 1980 A. Some of the very weakest lines may not be real. These are indicated by question marks. We also discuss some of the plasma diagnostics available using spectral lines of O v and O iv.

Spectrally-resolved measurements of aerosol extinction at ultraviolet and visible wavelengths

NASA Astrophysics Data System (ADS)

Flores, M.; Washenfelder, R. A.; Brock, C. A.; Brown, S. S.; Rudich, Y.

2012-12-01

Aerosols play an important role in the Earth's radiative budget. Aerosol extinction includes both the scattering and absorption of light, and these vary with wavelength, aerosol diameter, and aerosol composition. Historically, aerosol absorption has been measured using filter-based or extraction methods that are prone to artifacts. There have been few investigations of ambient aerosol optical properties at the blue end of the visible spectrum and into the ultraviolet. Brown carbon is particularly important in this spectral region, because it both absorbs and scatters light, and encompasses a large and variable group of organic compounds from biomass burning and secondary organic aerosol. We have developed a laboratory instrument that combines new, high-power LED light sources with high-finesse optical cavities to achieve sensitive measurements of aerosol optical extinction. This instrument contains two broadband channels, with spectral coverage from 360 - 390 nm and 385 - 420 nm. Using this instrument, we report aerosol extinction in the ultraviolet and near-visible spectral region as a function of chemical composition and structure. We have measured the extinction cross-sections between 360 - 420 nm with 0.5 nm resolution using different sizes and concentrations of polystyrene latex spheres, ammonium sulfate, and Suwannee River fulvic acid. Fitting the real and imaginary part of the refractive index allows the absorption and scattering to be determined.

Multi-spectral confocal microendoscope for in-vivo imaging

NASA Astrophysics Data System (ADS)

Rouse, Andrew Robert

The concept of in-vivo multi-spectral confocal microscopy is introduced. A slit-scanning multi-spectral confocal microendoscope (MCME) was built to demonstrate the technique. The MCME employs a flexible fiber-optic catheter coupled to a custom built slit-scan confocal microscope fitted with a custom built imaging spectrometer. The catheter consists of a fiber-optic imaging bundle linked to a miniature objective and focus assembly. The design and performance of the miniature objective and focus assembly are discussed. The 3mm diameter catheter may be used on its own or routed though the instrument channel of a commercial endoscope. The confocal nature of the system provides optical sectioning with 3mum lateral resolution and 30mum axial resolution. The prism based multi-spectral detection assembly is typically configured to collect 30 spectral samples over the visible chromatic range. The spectral sampling rate varies from 4nm/pixel at 490nm to 8nm/pixel at 660nm and the minimum resolvable wavelength difference varies from 7nm to 18nm over the same spectral range. Each of these characteristics are primarily dictated by the dispersive power of the prism. The MCME is designed to examine cellular structures during optical biopsy and to exploit the diagnostic information contained within the spectral domain. The primary applications for the system include diagnosis of disease in the gastro-intestinal tract and female reproductive system. Recent data from the grayscale imaging mode are presented. Preliminary multi-spectral results from phantoms, cell cultures, and excised human tissue are presented to demonstrate the potential of in-vivo multi-spectral imaging.

Multi-octave spectral beam combiner on ultra-broadband photonic integrated circuit platform.

PubMed

Stanton, Eric J; Heck, Martijn J R; Bovington, Jock; Spott, Alexander; Bowers, John E

2015-05-04

We present the design of a novel platform that is able to combine optical frequency bands spanning 4.2 octaves from ultraviolet to mid-wave infrared into a single, low M2 output waveguide. We present the design and realization of a key component in this platform that combines the wavelength bands of 350 nm - 1500 nm and 1500 nm - 6500 nm with demonstrated efficiency greater than 90% in near-infrared and mid-wave infrared. The multi-octave spectral beam combiner concept is realized using an integrated platform with silicon nitride waveguides and silicon waveguides. Simulated bandwidth is shown to be over four octaves, and measured bandwidth is shown over two octaves, limited by the availability of sources.

Magnetic fluorescent lamp

DOEpatents

Berman, S.M.; Richardson R.W.

1983-12-29

The radiant emission of a mercury-argon discharge in a fluorescent lamp assembly is enhanced by providing means for establishing a magnetic field with lines of force along the path of electron flow through the bulb of the lamp assembly, to provide Zeeman splitting of the ultraviolet spectral line. Optimum results are obtained when the magnetic field strength causes a Zeeman splitting of approximately 1.7 times the thermal line width.

The LUVOIR Ultraviolet Multi-Object Spectrograph (LUMOS): instrument definition and design

NASA Astrophysics Data System (ADS)

France, Kevin; Fleming, Brian; West, Garrett; McCandliss, Stephan R.; Bolcar, Matthew R.; Harris, Walter; Moustakas, Leonidas; O'Meara, John M.; Pascucci, Ilaria; Rigby, Jane; Schiminovich, David; Tumlinson, Jason

2017-08-01

The Large Ultraviolet/Optical/Infrared Surveyor (LUVOIR) is one of four large mission concepts currently undergoing community study for consideration by the 2020 Astronomy and Astrophysics Decadal Survey. LUVOIR is being designed to pursue an ambitious program of exoplanetary discovery and characterization, cosmic origins astrophysics, and planetary science. The LUVOIR study team is investigating two large telescope apertures (9- and 15-meter primary mirror diameters) and a host of science instruments to carry out the primary mission goals. Many of the exoplanet, cosmic origins, and planetary science goals of LUVOIR require high-throughput, imaging spectroscopy at ultraviolet (100 - 400 nm) wavelengths. The LUVOIR Ultraviolet Multi-Object Spectrograph, LUMOS, is being designed to support all of the UV science requirements of LUVOIR, from exoplanet host star characterization to tomography of circumgalactic halos to water plumes on outer solar system satellites. LUMOS offers point source and multi-object spectroscopy across the UV bandpass, with multiple resolution modes to support different science goals. The instrument will provide low (R = 8,000 - 18,000) and medium (R = 30,000 - 65,000) resolution modes across the far-ultraviolet (FUV: 100 - 200 nm) and nearultraviolet (NUV: 200 - 400 nm) windows, and a very low resolution mode (R = 500) for spectroscopic investigations of extremely faint objects in the FUV. Imaging spectroscopy will be accomplished over a 3 × 1.6 arcminute field-of-view by employing holographically-ruled diffraction gratings to control optical aberrations, microshutter arrays (MSA) built on the heritage of the Near Infrared Spectrograph (NIRSpec) on the James Webb Space Telescope (JWST), advanced optical coatings for high-throughput in the FUV, and next generation large-format photon-counting detectors. The spectroscopic capabilities of LUMOS are augmented by an FUV imaging channel (100 - 200nm, 13 milliarcsecond angular resolution, 2 × 2

Quantifying the Dynamics of Bacterial Secondary Metabolites by Spectral Multi-Photon Microscopy

PubMed Central

Sullivan, Nora L.; Tzeranis, Dimitrios S.; Wang, Yun; So, Peter T.C.; Newman, Dianne

2011-01-01

Phenazines, a group of fluorescent small molecules produced by the bacterium Pseudomonas aeruginosa, play a role in maintaining cellular redox homeostasis. Phenazines have been challenging to study in vivo due to their redox activity, presence both intra- and extracellularly, and their diverse chemical properties. Here, we describe a non-invasive in vivo optical technique to monitor phenazine concentrations within bacterial cells using time-lapsed spectral multi-photon fluorescence microscopy. This technique enables simultaneous monitoring of multiple weakly-fluorescent molecules (phenazines, siderophores, NAD(P)H) expressed by bacteria in culture. This work provides the first in vivo measurements of reduced phenazine concentration as well as the first description of the temporal dynamics of the phenazine-NAD(P)H redox system in Pseudomonas aeruginosa, illuminating an unanticipated role for 1-hydroxyphenazine. Similar approaches could be used to study the abundance and redox dynamics of a wide range of small molecules within bacteria, both as single cells and in communities. PMID:21671613

Retinex Preprocessing for Improved Multi-Spectral Image Classification

NASA Technical Reports Server (NTRS)

Thompson, B.; Rahman, Z.; Park, S.

2000-01-01

The goal of multi-image classification is to identify and label "similar regions" within a scene. The ability to correctly classify a remotely sensed multi-image of a scene is affected by the ability of the classification process to adequately compensate for the effects of atmospheric variations and sensor anomalies. Better classification may be obtained if the multi-image is preprocessed before classification, so as to reduce the adverse effects of image formation. In this paper, we discuss the overall impact on multi-spectral image classification when the retinex image enhancement algorithm is used to preprocess multi-spectral images. The retinex is a multi-purpose image enhancement algorithm that performs dynamic range compression, reduces the dependence on lighting conditions, and generally enhances apparent spatial resolution. The retinex has been successfully applied to the enhancement of many different types of grayscale and color images. We show in this paper that retinex preprocessing improves the spatial structure of multi-spectral images and thus provides better within-class variations than would otherwise be obtained without the preprocessing. For a series of multi-spectral images obtained with diffuse and direct lighting, we show that without retinex preprocessing the class spectral signatures vary substantially with the lighting conditions. Whereas multi-dimensional clustering without preprocessing produced one-class homogeneous regions, the classification on the preprocessed images produced multi-class non-homogeneous regions. This lack of homogeneity is explained by the interaction between different agronomic treatments applied to the regions: the preprocessed images are closer to ground truth. The principle advantage that the retinex offers is that for different lighting conditions classifications derived from the retinex preprocessed images look remarkably "similar", and thus more consistent, whereas classifications derived from the original

Thin film optical coatings for the ultraviolet spectral region

NASA Astrophysics Data System (ADS)

Torchio, P.; Albrand, G.; Alvisi, M.; Amra, C.; Rauf, H.; Cousin, B.; Otrio, G.

2017-11-01

The applications and innovations related to the ultraviolet field are today in strong growth. To satisfy these developments which go from biomedical to the large equipment like the Storage Ring Free Electron Laser, it is crucial to control with an extreme precision the optical performances, in using the substrates and the thin film materials impossible to circumvent in this spectral range. In particular, the reduction of the losses by electromagnetic diffusion, Joule effect absorption, or the behavior under UV luminous flows of power, resistance to surrounding particulate flows... become top priority which concerns a broad European and international community. Our laboratory has the theoretical, experimental and technological tools to design and fabricate numerous multilayer coatings with desirable optical properties in the visible and infrared spectral ranges. We have extended our expertise to the ultraviolet. We present here some results on high reflectivity multidielectric mirrors towards 250 nm in wavelength, produced by Ion Plating Deposition. The latter technique allows us to obtain surface treatments with low absorption and high resistance. We give in this study the UV transparent materials and the manufacturing technology which have been the best suited to meet requirements. Single UV layers were deposited and characterized. HfO2/SiO2 mirrors with a reflectance higher than 99% at 300 nm were obtained. Optical and non-optical characterizations such as UV spectrophotometric measurements, X-Ray Diffraction spectra, Scanning Electron Microscope and Atomic Force Microscope images were performed

Constraints on the temperature inhomogeneity in quasar accretion discs from the ultraviolet-optical spectral variability

NASA Astrophysics Data System (ADS)

Kokubo, Mitsuru

2015-05-01

The physical mechanisms of the quasar ultraviolet (UV)-optical variability are not well understood despite the long history of observations. Recently, Dexter & Agol presented a model of quasar UV-optical variability, which assumes large local temperature fluctuations in the quasar accretion discs. This inhomogeneous accretion disc model is claimed to describe not only the single-band variability amplitude, but also microlensing size constraints and the quasar composite spectral shape. In this work, we examine the validity of the inhomogeneous accretion disc model in the light of quasar UV-optical spectral variability by using five-band multi-epoch light curves for nearly 9 000 quasars in the Sloan Digital Sky Survey (SDSS) Stripe 82 region. By comparing the values of the intrinsic scatter σint of the two-band magnitude-magnitude plots for the SDSS quasar light curves and for the simulated light curves, we show that Dexter & Agol's inhomogeneous accretion disc model cannot explain the tight inter-band correlation often observed in the SDSS quasar light curves. This result leads us to conclude that the local temperature fluctuations in the accretion discs are not the main driver of the several years' UV-optical variability of quasars, and consequently, that the assumption that the quasar accretion discs have large localized temperature fluctuations is not preferred from the viewpoint of the UV-optical spectral variability.

Characterisation of spectrophotometers used for spectral solar ultraviolet radiation measurements.

PubMed

Gröbner, J

2001-01-01

Spectrophotometers used for spectral measurements of the solar ultraviolet radiation need to be well characterised to provide accurate and reliable data. Since the characterisation and calibration are usually performed in the laboratory under conditions very different from those encountered during solar measurements, it is essential to address all issues concerned with the representativity of the laboratory characterisation with respect to the solar measurements. These include among others the instrument stability, the instrument linearity, the instrument responsivity, the wavelength accuracy, the spectral resolution, stray light rejection and the instrument dependence on ambient temperature fluctuations. These instrument parameters need to be determined often enough so that the instrument changes only marginally in the period between successive characterisations and therefore provides reliable data for the intervening period.

Spectrally resolved visualization of fluorescent dyes permeating into skin

NASA Astrophysics Data System (ADS)

Maeder, Ulf; Bergmann, Thorsten; Beer, Sebastian; Burg, Jan Michael; Schmidts, Thomas; Runkel, Frank; Fiebich, Martin

2012-03-01

We present a spectrally resolved confocal imaging approach to qualitatively asses the overall uptake and the penetration depth of fluorescent dyes into biological tissue. We use a confocal microscope with a spectral resolution of 5 nm to measure porcine skin tissue after performing a Franz-Diffusion experiment with a submicron emulsion enriched with the fluorescent dye Nile Red. The evaluation uses linear unmixing of the dye and the tissue autofluorescence spectra. The results are combined with a manual segmentation of the skin's epidermis and dermis layers to assess the penetration behavior additionally to the overall uptake. The diffusion experiments, performed for 3h and 24h, show a 3-fold increased dye uptake in the epidermis and dermis for the 24h samples. As the method is based on spectral information it does not face the problem of superimposed dye and tissue spectra and therefore is more precise compared to intensity based evaluation methods.

Extreme ultraviolet spectral irradiance measurements since 1946

NASA Astrophysics Data System (ADS)

Schmidtke, G.

2015-03-01

In the physics of the upper atmosphere the solar extreme ultraviolet (EUV) radiation plays a dominant role controlling most of the thermospheric/ionospheric (T/I) processes. Since this part of the solar spectrum is absorbed in the thermosphere, platforms to measure the EUV fluxes became only available with the development of rockets reaching altitude levels exceeding 80 km. With the availability of V2 rockets used in space research, recording of EUV spectra started in 1946 using photographic films. The development of pointing devices to accurately orient the spectrographs toward the sun initiated intense activities in solar-terrestrial research. The application of photoelectric recording technology enabled the scientists placing EUV spectrometers aboard satellites observing qualitatively strong variability of the solar EUV irradiance on short-, medium-, and long-term scales. However, as more measurements were performed more radiometric EUV data diverged due to the inherent degradation of the EUV instruments with time. Also, continuous recording of the EUV energy input to the T/I system was not achieved. It is only at the end of the last century that there was progress made in solving the serious problem of degradation enabling to monitore solar EUV fluxes with sufficient radiometric accuracy. The data sets available allow composing the data available to the first set of EUV data covering a period of 11 years for the first time. Based on the sophisticated instrumentation verified in space, future EUV measurements of the solar spectral irradiance (SSI) are promising accuracy levels of about 5% and less. With added low-cost equipment, real-time measurements will allow providing data needed in ionospheric modeling, e.g., for correcting propagation delays of navigation signals from space to earth. Adding EUV airglow and auroral emission monitoring by airglow cameras, the impact of space weather on the terrestrial T/I system can be studied with a spectral terrestrial

Far-ultraviolet fluorescence of carbon monoxide in the red giant Arcturus

NASA Technical Reports Server (NTRS)

Ayres, T. R.; Moos, H. W.; Linsky, J. L.

1981-01-01

Evidence is presented that many of the weak features observed with International Ultraviolet Explorer (IUE) in the far-ultraviolet (1150-2000 A) spectrum of the archetype red giant Arcturus (K2 III) are A-X fourth positive bands of carbon monoxide excited by chromospheric emissions of O I, C I, and H I. The appearance of fluorescent CO bands near the wavelength of commonly used indicators of high-temperature (T greater than 20,000 K) plasma, such as C II at wavelength 1335 and C IV at wavelength 1548, introduces a serious ambiguity in diagnosing the presence of hot material in the outer atmospheres of the cool giants by means of low-dispersion IUE spectra.

SERS-fluorescence joint spectral encoded magnetic nanoprobes for multiplex cancer cell separation.

PubMed

Wang, Zhuyuan; Zong, Shenfei; Chen, Hui; Wang, Chunlei; Xu, Shuhong; Cui, Yiping

2014-11-01

A new kind of cancer cell separation method is demonstrated, using surface-enhanced Raman scattering (SERS) and fluorescence dual-encoded magnetic nanoprobes. The designed nanoprobes can realize SERS-fluorescence joint spectral encoding (SFJSE) and greatly improve the multiplexing ability. The nanoprobes have four main components, that is, the magnetic core, SERS generator, fluorescent agent, and targeting antibody. These components are assembled with a multi-layered structure to form the nanoprobes. Specifically, silica-coated magnetic nanobeads (MBs) are used as the inner core. Au core-Ag shell nanorods (Au@Ag NRs) are employed as the SERS generators and attached on the silica-coated MBs. After burying these Au@Ag NRs with another silica layer, CdTe quantum dots (QDs), that is, the fluorescent agent, are anchored onto the silica layer. Finally, antibodies are covalently linked to CdTe QDs. SFJSE is fulfilled by using different Raman molecules and QDs with different emission wavelengths. By utilizing four human cancer cell lines and one normal cell line as the model cells, the nanoprobes can specifically and simultaneously separate target cancer cells from the normal ones. This SFJSE-based method greatly facilitates the multiplex, rapid, and accurate cancer cell separation, and has a prosperous potential in high-throughput analysis and cancer diagnosis. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Detection of melanomas by digital imaging of spectrally resolved UV light-induced autofluorescence of human skin

NASA Astrophysics Data System (ADS)

Chwirot, B. W.; Chwirot, S.; Jedrzejczyk, W.; Redzinski, J.; Raczynska, A. M.; Telega, K.

2001-07-01

We studied spectral and spatial distributions of the intensity of the ultraviolet light-excited fluorescence of human skin. Our studied performed in situ in 162 patients with malignant and non-malignant skin lesions resulted in a new method of detecting melanomas in situ using digital imaging of the spectrally resolved fluorescence. With our diagnostic algorithm we could successfully detect 88.5% of the cases of melanoma in the group of patients subject to examinations with the fluorescence method. A patent application for the method has been submitted to the Patent Office in Warsaw.

Dancing to the MUSSIC: Steps towards creating a Multisatellite Ultraviolet Solar Spectral Irradiance Composite

NASA Astrophysics Data System (ADS)

Snow, M. A.; Machol, J. L.; Richard, E. C.

2016-12-01

Solar spectral irradiance (SSI) has been measured since the beginning of the satellite era in 1978, but the observational record has many gaps in both wavelength and time. We describe our current effort in linking several such datasets ranging from the Extreme Ultraviolet to the Near Ultraviolet (0-400 nm). This wavelength range includes two important solar activity proxies, the Magnesium II core—to-wing ratio and the Lyman alpha irradiance, and special attention will be applied to these two wavelength intervals.

Multi-site recording and spectral analysis of spontaneous photon emission from human body.

PubMed

Wijk, Eduard P A Van; Wijk, Roeland Van

2005-04-01

In the past years, research on ultraweak photon emission (UPE) from human body has increased for isolated cells and tissues. However, there are only limited data on UPE from the whole body, in particular from the hands. To describe a protocol for the management of subjects that (1) avoids interference with light-induced longterm delayed luminescence, and (2) includes the time slots for recording photon emission. The protocol was utilised for multi-site recording of 4 subjects at different times of the day and different seasons, and for one subject to complete spectral analysis of emission from different body locations. An especially selected low-noise end-window photomultiplier was utilised for the detection of ultraviolet / visible light (200-650 nm) photon emission. For multi-site recording it was manipulated in three directions in a darkroom with a very low count rate. A series of cut-off filters was used for spectral analysis of UPE. 29 body sites were selected such that the distribution in UPE could be studied as right-left symmetry, dorsal-ventral symmetry, and the ratio between the central body part and extremities. Generally, the fluctuation in photon counts over the body was lower in the morning than in the afternoon. The thorax-abdomen region emitted lowest and most constantly. The upper extremities and the head region emitted most and increasingly over the day. Spectral analysis of low, intermediate and high emission from the superior frontal part of the right leg, the forehead and the palms in the sensitivity range of the photomultiplier showed the major spontaneous emission at 470-570 nm. The central palm area of hand emission showed a larger contribution of the 420-470 nm range in the spectrum of spontaneous emission from the hand in autumn/winter. The spectrum of delayed luminescence from the hand showed major emission in the same range as spontaneous emission. Examples of multi-site UPE recordings and spectral analysis revealed individual patterns

Fluorescence based spectral assessment of pork meat freshness

USDA-ARS?s Scientific Manuscript database

Development of sensitive, nondestructive measurement methods for meat freshness is necessary to ensure safe distribution of meat products in the continually growing meat market. Fluorescence spectral technology has been shown to be a promising measurement method for quality and safety evaluation of ...

Kinetics of T-cell receptor-dependent antigen recognition determined in vivo by multi-spectral normalized epifluorescence laser scanning

NASA Astrophysics Data System (ADS)

Favicchio, Rosy; Zacharakis, Giannis; Oikonomaki, Katerina; Zacharopoulos, Athanasios; Mamalaki, Clio; Ripoll, Jorge

2012-07-01

Detection of multiple fluorophores in conditions of low signal represents a limiting factor for the application of in vivo optical imaging techniques in immunology where fluorescent labels report for different functional characteristics. A noninvasive in vivo Multi-Spectral Normalized Epifluorescence Laser scanning (M-SNELS) method was developed for the simultaneous and quantitative detection of multiple fluorophores in low signal to noise ratios and used to follow T-cell activation and clonal expansion. Colocalized DsRed- and GFP-labeled T cells were followed in tandem during the mounting of an immune response. Spectral unmixing was used to distinguish the overlapping fluorescent emissions representative of the two distinct cell populations and longitudinal data reported the discrete pattern of antigen-driven proliferation. Retrieved values were validated both in vitro and in vivo with flow cytometry and significant correlation between all methodologies was achieved. Noninvasive M-SNELS successfully quantified two colocalized fluorescent populations and provides a valid alternative imaging approach to traditional invasive methods for detecting T cell dynamics.

Fluorescent marker-based and marker-free discrimination between healthy and cancerous human tissues using hyper-spectral imaging

NASA Astrophysics Data System (ADS)

Arnold, Thomas; De Biasio, Martin; Leitner, Raimund

2015-06-01

Two problems are addressed in this paper (i) the fluorescent marker-based and the (ii) marker-free discrimination between healthy and cancerous human tissues. For both applications the performance of hyper-spectral methods are quantified. Fluorescent marker-based tissue classification uses a number of fluorescent markers to dye specific parts of a human cell. The challenge is that the emission spectra of the fluorescent dyes overlap considerably. They are, furthermore disturbed by the inherent auto-fluorescence of human tissue. This results in ambiguities and decreased image contrast causing difficulties for the treatment decision. The higher spectral resolution introduced by tunable-filter-based spectral imaging in combination with spectral unmixing techniques results in an improvement of the image contrast and therefore more reliable information for the physician to choose the treatment decision. Marker-free tissue classification is based solely on the subtle spectral features of human tissue without the use of artificial markers. The challenge in this case is that the spectral differences between healthy and cancerous tissues are subtle and embedded in intra- and inter-patient variations of these features. The contributions of this paper are (i) the evaluation of hyper-spectral imaging in combination with spectral unmixing techniques for fluorescence marker-based tissue classification, (ii) the evaluation of spectral imaging for marker-free intra surgery tissue classification. Within this paper, we consider real hyper-spectral fluorescence and endoscopy data sets to emphasize the practical capability of the proposed methods. It is shown that the combination of spectral imaging with multivariate statistical methods can improve the sensitivity and specificity of the detection and the staging of cancerous tissues compared to standard procedures.

In vitro fluorescence measurements and Monte Carlo simulation of laser irradiation propagation in porcine skin tissue.

PubMed

Drakaki, E; Makropoulou, M; Serafetinides, A A

2008-07-01

In dermatology, the in vivo spectral fluorescence measurements of human skin can serve as a valuable supplement to standard non-invasive techniques for diagnosing various skin diseases. However, quantitative analysis of the fluorescence spectra is complicated by the fact that skin is a complex multi-layered and inhomogeneous organ, with varied optical properties and biophysical characteristics. In this work, we recorded, in vitro, the laser-induced fluorescence emission signals of healthy porcine skin, one of the animals, which is considered as one of the most common models for investigations related to medical diagnostics of human cutaneous tissues. Differences were observed in the form and intensity of the fluorescence signal of the porcine skin, which can be attributed to the different concentrations of the native fluorophores and the variable physical and biological conditions of the skin tissue. As the light transport in the tissue target is directly influencing the absorption and the fluorescence emission signals, we performed Monte Carlo simulation of the light distribution in a five-layer model of human skin tissue, with a pulsed ultraviolet laser beam.

Polymer-and glass-based fluorescence standards for the near infrared (NIR) spectral region.

PubMed

Würth, Christian; Hoffmann, Katrin; Behnke, Thomas; Ohnesorge, Marius; Resch-Genger, Ute

2011-05-01

The widespread use and acceptance of fluorescence techniques especially in regulated areas like medical diagnostics is closely linked to standardization concepts that guarantee and improve the comparability and reliability of fluorescence measurements. At the core of such concepts are dependable fluorescence standards that are preferably certified. The ever rising interest in fluorescence measurements in the near-infrared (NIR) spectral region renders the availability of spectral and intensity standards for this wavelength region increasingly important. This encouraged us to develop approaches to solid NIR standards based upon dye-doped polymers and assess their application-relevant properties in comparison to metal ion-doped glasses. The overall goal is here to provide inexpensive, easily fabricated, and robust internal and external calibration tools for a broad variety of fluorescence instruments ranging e.g. from spectrofluorometers over fluorescence microscopes to miniaturized fluorescence sensors. © Springer Science+Business Media, LLC 2010

Semiconductor Laser Multi-Spectral Sensing and Imaging

PubMed Central

Le, Han Q.; Wang, Yang

2010-01-01

Multi-spectral laser imaging is a technique that can offer a combination of the laser capability of accurate spectral sensing with the desirable features of passive multispectral imaging. The technique can be used for detection, discrimination, and identification of objects by their spectral signature. This article describes and reviews the development and evaluation of semiconductor multi-spectral laser imaging systems. Although the method is certainly not specific to any laser technology, the use of semiconductor lasers is significant with respect to practicality and affordability. More relevantly, semiconductor lasers have their own characteristics; they offer excellent wavelength diversity but usually with modest power. Thus, system design and engineering issues are analyzed for approaches and trade-offs that can make the best use of semiconductor laser capabilities in multispectral imaging. A few systems were developed and the technique was tested and evaluated on a variety of natural and man-made objects. It was shown capable of high spectral resolution imaging which, unlike non-imaging point sensing, allows detecting and discriminating objects of interest even without a priori spectroscopic knowledge of the targets. Examples include material and chemical discrimination. It was also shown capable of dealing with the complexity of interpreting diffuse scattered spectral images and produced results that could otherwise be ambiguous with conventional imaging. Examples with glucose and spectral imaging of drug pills were discussed. Lastly, the technique was shown with conventional laser spectroscopy such as wavelength modulation spectroscopy to image a gas (CO). These results suggest the versatility and power of multi-spectral laser imaging, which can be practical with the use of semiconductor lasers. PMID:22315555

Semiconductor laser multi-spectral sensing and imaging.

PubMed

Le, Han Q; Wang, Yang

2010-01-01

Multi-spectral laser imaging is a technique that can offer a combination of the laser capability of accurate spectral sensing with the desirable features of passive multispectral imaging. The technique can be used for detection, discrimination, and identification of objects by their spectral signature. This article describes and reviews the development and evaluation of semiconductor multi-spectral laser imaging systems. Although the method is certainly not specific to any laser technology, the use of semiconductor lasers is significant with respect to practicality and affordability. More relevantly, semiconductor lasers have their own characteristics; they offer excellent wavelength diversity but usually with modest power. Thus, system design and engineering issues are analyzed for approaches and trade-offs that can make the best use of semiconductor laser capabilities in multispectral imaging. A few systems were developed and the technique was tested and evaluated on a variety of natural and man-made objects. It was shown capable of high spectral resolution imaging which, unlike non-imaging point sensing, allows detecting and discriminating objects of interest even without a priori spectroscopic knowledge of the targets. Examples include material and chemical discrimination. It was also shown capable of dealing with the complexity of interpreting diffuse scattered spectral images and produced results that could otherwise be ambiguous with conventional imaging. Examples with glucose and spectral imaging of drug pills were discussed. Lastly, the technique was shown with conventional laser spectroscopy such as wavelength modulation spectroscopy to image a gas (CO). These results suggest the versatility and power of multi-spectral laser imaging, which can be practical with the use of semiconductor lasers.

COMPARATIVE TOXICITY OF FLUORANTHENE TO FRESHWATER AND SALTWATER SPECIES UNDER FLUORESCENT AND ULTRAVIOLET LIGHT

EPA Science Inventory

The acute and chronic toxicity of fluoranthene was determined for a diverse group of freshwater and saltwater species under both standard laboratory fluorescent light and ultraviolet (UV) light test conditions. Acute tests with 21 species demonstrated that fluoranthene was not le...

Development of a rapid method for the automatic classification of biological agents' fluorescence spectral signatures

NASA Astrophysics Data System (ADS)

Carestia, Mariachiara; Pizzoferrato, Roberto; Gelfusa, Michela; Cenciarelli, Orlando; Ludovici, Gian Marco; Gabriele, Jessica; Malizia, Andrea; Murari, Andrea; Vega, Jesus; Gaudio, Pasquale

2015-11-01

Biosecurity and biosafety are key concerns of modern society. Although nanomaterials are improving the capacities of point detectors, standoff detection still appears to be an open issue. Laser-induced fluorescence of biological agents (BAs) has proved to be one of the most promising optical techniques to achieve early standoff detection, but its strengths and weaknesses are still to be fully investigated. In particular, different BAs tend to have similar fluorescence spectra due to the ubiquity of biological endogenous fluorophores producing a signal in the UV range, making data analysis extremely challenging. The Universal Multi Event Locator (UMEL), a general method based on support vector regression, is commonly used to identify characteristic structures in arrays of data. In the first part of this work, we investigate fluorescence emission spectra of different simulants of BAs and apply UMEL for their automatic classification. In the second part of this work, we elaborate a strategy for the application of UMEL to the discrimination of different BAs' simulants spectra. Through this strategy, it has been possible to discriminate between these BAs' simulants despite the high similarity of their fluorescence spectra. These preliminary results support the use of SVR methods to classify BAs' spectral signatures.

Quantitative method to assess caries via fluorescence imaging from the perspective of autofluorescence spectral analysis

NASA Astrophysics Data System (ADS)

Chen, Q. G.; Zhu, H. H.; Xu, Y.; Lin, B.; Chen, H.

2015-08-01

A quantitative method to discriminate caries lesions for a fluorescence imaging system is proposed in this paper. The autofluorescence spectral investigation of 39 teeth samples classified by the International Caries Detection and Assessment System levels was performed at 405 nm excitation. The major differences in the different caries lesions focused on the relative spectral intensity range of 565-750 nm. The spectral parameter, defined as the ratio of wavebands at 565-750 nm to the whole spectral range, was calculated. The image component ratio R/(G + B) of color components was statistically computed by considering the spectral parameters (e.g. autofluorescence, optical filter, and spectral sensitivity) in our fluorescence color imaging system. Results showed that the spectral parameter and image component ratio presented a linear relation. Therefore, the image component ratio was graded as <0.66, 0.66-1.06, 1.06-1.62, and >1.62 to quantitatively classify sound, early decay, established decay, and severe decay tissues, respectively. Finally, the fluorescence images of caries were experimentally obtained, and the corresponding image component ratio distribution was compared with the classification result. A method to determine the numerical grades of caries using a fluorescence imaging system was proposed. This method can be applied to similar imaging systems.

Marine fluorescence from high spectrally resolved satellite measurements

NASA Astrophysics Data System (ADS)

Wolanin, Aleksandra; Dinter, Tilman; Rozanov, Vladimir; Noël, Stefan; Vountas, Marco; Burrows, John P.; Bracher, Astrid

2014-05-01

When chlorophyll molecules absorb light, most of this energy is transformed into chemical energy in a process of photosynthesis. However, a fraction of the energy absorbed is reemitted as fluorescence. As a result of its relationship to photosynthetic e?ciency, information about chlorophyll fluorescence can be used to assess the physiological state of phytoplankton (Falkowski and Kolber,1995). In-situ measurements of chlorophyll fluorescence are widespread in physiological and ecophysiological studies. When retrieved from space, chlorophyll fluorescence can improve our knowledge of global biogeochemical cycles and phytoplankton productivity (Behrenfeld et al., 2009; Huot et al., 2013) by providing high coverage and periodicity. So far, the only satellite retrieval of sun-induced marine fluorescence, Fluorescence Line Height (FLH), was designed for MODIS (Abbott and Letelier, 1999), and later also applied to the similar sensor MERIS (Gower et al., 2004). However, it could so far not be evaluated on global scale. Here, we present a different approach to observe marine chlorophyll fluorescence, based on the Differential Optical Absorption Spectroscopy (DOAS) technique (Perner and Platt, 1979) applied to the hyperspectral data from Scanning Imaging Absorption Spectrometer for Atmospheric Chartography (SCIAMACHY) and Global Ozone Monitoring Experiment-2 (GOME-2). Since fluorescence, as a trans-spectral process, leads to the shift of the wavelength of the radiation, it can be observed in the filling-in of Fraunhofer lines. In our retrieval, we evaluate the filling-in of the Zeeman triplet Fraunhofer line FeI at 684.3 nm, which is located very close to the emission peak of marine fluorescence (~685 nm). In order to conduct the chlorophyll fluorescence retrieval with the DOAS method, we calculated the reference spectra for chlorophyll fluorescence, based on simulations performed with the coupled ocean-atmosphere radiative transfer model SCIATRAN (Rozanov et al., 2014

Fluorescence spectra of blood plasma treated with ultraviolet irradiation in vivo

NASA Astrophysics Data System (ADS)

Zalesskaya, G. A.; Maslova, T. O.

2010-09-01

We have studied the fluorescence spectra of blood plasma from patients with acute coronary syndrome, and also the effect of therapeutic doses of in vivo ultraviolet blood irradiation (UBI) on the spectra. We have established that the maxima in the fluorescence spectra of the original plasma samples, obtained from unirradiated blood, are located in the wavelength interval 330-340 nm, characteristic for the fluorescence of tryptophan residues. In extracorporeal UBI ( λ = 254 nm), we observed changes in the shape and also both a blue and a red shift in the maxima of the fluorescence spectra, differing in magnitude for blood plasma samples from different patients in the test group. We show that UBI-initiated changes in the fluorescence spectra of the plasma depend on the original pathological disturbances of metabolite levels, and also on the change in the oxygen-transport function of the blood and the acid-base balance, affecting the oxidative stability of the plasma. We have concluded that UV irradiation, activating buffer systems in the blood, has an effect on the universal and specific interactions of the tryptophan residue with the amino acid residues and water surrounding it.

[Investigation of quantitative detection of water quality using spectral fluorescence signature].

PubMed

He, Jun-hua; Cheng, Yong-jin; Han, Yan-ling; Zhang, Hao; Yang, Tao

2008-08-01

A method of spectral analysis, which can simultaneously detect dissolved organic matter (DOM) and chlorophyll a (Chl-a) in natural water, was developed in the present paper with the intention of monitoring water quality fast and quantitatively. Firstly, the total luminescence spectra (TLS) of water sample from East Lake in Wuhan city were measured by the use of laser (532 nm) induced fluorescence (LIF). There were obvious peaks of relative intensity at the wavelength value of 580, 651 and 687 nm in the TLS of the sample, which correspond respectively to spectra of DOM, and the Raman scattering of water and Chl-a in the water. Then the spectral fluorescence signature (SFS) technique was adopted to analyze and distinguish spectral characteristics of DOM and Chl-a in natural water. The calibration curves and function expressions, which indicate the relation between the normalized fluorescence intensities of DOM and Chl-a in water and their concentrations, were obtained respectively under the condition of low concentration(< 40 mg x L(-1))by using normalization of Raman scattering spectrum of water. The curves have a high linearity. When the concentration of the solution with humic acid is large (> 40 mg x L(-1)), the Raman scattering signal is totally absorbed by the molecules of humic acid being on the ground state, so the normalization technique can not be adopted. However the function expression between the concentration of the solution with humic acid and its relative fluorescence peak intensity can be acquired directly with the aid of experiment of fluorescence spectrum. It is concluded that although the expression is non-linearity as a whole, there is a excellent linear relation between the fluorescence intensity and concentration of DOM when the concentration is less than 200 mg x L(-1). The method of measurement based on spectral fluorescence signature technique and the calibration curves gained will have prospects of broad application. It can recognize fast

Spectral fluorescent properties of tissues in vivo with excitation in the red wavelength range

NASA Astrophysics Data System (ADS)

Stratonnikov, Alexander A.; Loschenov, Victor B.; Klimov, D. V.; Edinac, N. E.; Wolnukhin, V. A.; Strashkevich, I. A.

1997-12-01

The spectral fluorescence analysis is a promising method for differential tissue diagnostic. Usually the UV and visible light is used for fluorescence excitation with emission registration in the visible wavelength range. The light penetration length in this wavelength range is very small allowing one to analyze only the surface region of the tissue. Here we present the tissue fluorescent spectra in vivo excited in the red wavelength region. As excitation light source we used compact He-Ne laser (632.8 nm) and observed the fluorescence in 650 - 800 nm spectral range. The various tissues including normal skin, psoriasis, tumors, necrosis as well as photosensitized tissues have been measured.

Intelligent multi-spectral IR image segmentation

NASA Astrophysics Data System (ADS)

Lu, Thomas; Luong, Andrew; Heim, Stephen; Patel, Maharshi; Chen, Kang; Chao, Tien-Hsin; Chow, Edward; Torres, Gilbert

2017-05-01

This article presents a neural network based multi-spectral image segmentation method. A neural network is trained on the selected features of both the objects and background in the longwave (LW) Infrared (IR) images. Multiple iterations of training are performed until the accuracy of the segmentation reaches satisfactory level. The segmentation boundary of the LW image is used to segment the midwave (MW) and shortwave (SW) IR images. A second neural network detects the local discontinuities and refines the accuracy of the local boundaries. This article compares the neural network based segmentation method to the Wavelet-threshold and Grab-Cut methods. Test results have shown increased accuracy and robustness of this segmentation scheme for multi-spectral IR images.

Experimental recovery of intrinsic fluorescence and fluorophore concentration in the presence of hemoglobin: spectral effect of scattering and absorption on fluorescence

NASA Astrophysics Data System (ADS)

Du Le, Vinh Nguyen; Patterson, Michael S.; Farrell, Thomas J.; Hayward, Joseph E.; Fang, Qiyin

2015-12-01

The ability to recover the intrinsic fluorescence of biological fluorophores is crucial to accurately identify the fluorophores and quantify their concentrations in the media. Although some studies have successfully retrieved the fluorescence spectral shape of known fluorophores, the techniques usually came with heavy computation costs and did not apply for strongly absorptive media, and the intrinsic fluorescence intensity and fluorophore concentration were not recovered. In this communication, an experimental approach was presented to recover intrinsic fluorescence and concentration of fluorescein in the presence of hemoglobin (Hb). The results indicated that the method was efficient in recovering the intrinsic fluorescence peak and fluorophore concentration with an error of 3% and 10%, respectively. The results also suggested that chromophores with irregular absorption spectra (e.g., Hb) have more profound effects on fluorescence spectral shape than chromophores with monotonic absorption and scattering spectra (e.g., black India ink and polystyrene microspheres).

MAUVE/SWIPE: an imaging instrument concept with multi-angular, -spectral, and -polarized capability for remote sensing of aerosols, ocean color, clouds, and vegetation from space

NASA Astrophysics Data System (ADS)

Frouin, Robert; Deschamps, Pierre-Yves; Rothschild, Richard; Stephan, Edward; Leblanc, Philippe; Duttweiler, Fred; Ghaemi, Tony; Riedi, Jérôme

2006-12-01

The Monitoring Aerosols in the Ultraviolet Experiment (MAUVE) and the Short-Wave Infrared Polarimeter Experiment (SWIPE) instruments have been designed to collect, from a typical sun-synchronous polar orbit at 800 km altitude, global observations of the spectral, polarized, and directional radiance reflected by the earth-atmosphere system for a wide range of applications. Based on the heritage of the POLDER radiometer, the MAUVE/SWIPE instrument concept combines the merits of TOMS for observing in the ultra-violet, MISR for wide field-of-view range, MODIS, for multi-spectral aspects in the visible and near infrared, and the POLDER instrument for polarization. The instruments are camera systems with 2-dimensional detector arrays, allowing a 120-degree field-of-view with adequate ground resolution (i.e., 0.4 or 0.8 km at nadir) from satellite altitude. Multi-angle viewing is achieved by the along-track migration at spacecraft velocity of the 2-dimensional field-of-view. Between the cameras' optical assembly and detector array are two filter wheels, one carrying spectral filters, the other polarizing filters, allowing measurements of the first three Stokes parameters, I. Q, and V, of the incident radiation in 16 spectral bands optimally placed in the interval 350-2200 nm. The spectral range is 350-1050 nm for the MAUVE instrument and 1050-2200 nm for the SWIPE instrument. The radiometric requirements are defined to fully exploit the multi-angular, multi-spectral, and multi-polarized capability of the instruments. These include a wide dynamic range, a signal-to-noise ratio above 500 in all channels at maximum radiance level, i.e., when viewing a surface target of albedo equal to 1, and a noise-equivalent-differential reflectance better than 0.0005 at low signal level for a sun at zenith. To achieve daily global coverage, a pair of MAUVE and SWIPE instruments would be carried by each of two mini-satellites placed on interlaced orbits. The equator crossing time of the

Improved discrimination between monocotyledonous and dicotyledonous plants for weed control based on the blue-green region of ultraviolet-induced fluorescence spectra.

PubMed

Panneton, Bernard; Guillaume, Serge; Roger, Jean-Michel; Samson, Guy

2010-01-01

Precision weeding by spot spraying in real time requires sensors to discriminate between weeds and crop without contact. Among the optical based solutions, the ultraviolet (UV) induced fluorescence of the plants appears as a promising alternative. In a first paper, the feasibility of discriminating between corn hybrids, monocotyledonous, and dicotyledonous weeds was demonstrated on the basis of the complete spectra. Some considerations about the different sources of fluorescence oriented the focus to the blue-green fluorescence (BGF) part, ignoring the chlorophyll fluorescence that is inherently more variable in time. This paper investigates the potential of performing weed/crop discrimination on the basis of several large spectral bands in the BGF area. A partial least squares discriminant analysis (PLS-DA) was performed on a set of 1908 spectra of corn and weed plants over 3 years and various growing conditions. The discrimination between monocotyledonous and dicotyledonous plants based on the blue-green fluorescence yielded robust models (classification error between 1.3 and 4.6% for between-year validation). On the basis of the analysis of the PLS-DA model, two large bands were chosen in the blue-green fluorescence zone (400-425 nm and 425-490 nm). A linear discriminant analysis based on the signal from these two bands also provided very robust inter-year results (classification error from 1.5% to 5.2%). The same selection process was applied to discriminate between monocotyledonous weeds and maize but yielded no robust models (up to 50% inter-year error). Further work will be required to solve this problem and provide a complete UV fluorescence based sensor for weed-maize discrimination.

Principle component analysis and linear discriminant analysis of multi-spectral autofluorescence imaging data for differentiating basal cell carcinoma and healthy skin

NASA Astrophysics Data System (ADS)

Chernomyrdin, Nikita V.; Zaytsev, Kirill I.; Lesnichaya, Anastasiya D.; Kudrin, Konstantin G.; Cherkasova, Olga P.; Kurlov, Vladimir N.; Shikunova, Irina A.; Perchik, Alexei V.; Yurchenko, Stanislav O.; Reshetov, Igor V.

2016-09-01

In present paper, an ability to differentiate basal cell carcinoma (BCC) and healthy skin by combining multi-spectral autofluorescence imaging, principle component analysis (PCA), and linear discriminant analysis (LDA) has been demonstrated. For this purpose, the experimental setup, which includes excitation and detection branches, has been assembled. The excitation branch utilizes a mercury arc lamp equipped with a 365-nm narrow-linewidth excitation filter, a beam homogenizer, and a mechanical chopper. The detection branch employs a set of bandpass filters with the central wavelength of spectral transparency of λ = 400, 450, 500, and 550 nm, and a digital camera. The setup has been used to study three samples of freshly excised BCC. PCA and LDA have been implemented to analyze the data of multi-spectral fluorescence imaging. Observed results of this pilot study highlight the advantages of proposed imaging technique for skin cancer diagnosis.

Novel full-spectral flow cytometry with multiple spectrally-adjacent fluorescent proteins and fluorochromes and visualization of in vivo cellular movement.

PubMed

Futamura, Koji; Sekino, Masashi; Hata, Akihiro; Ikebuchi, Ryoyo; Nakanishi, Yasutaka; Egawa, Gyohei; Kabashima, Kenji; Watanabe, Takeshi; Furuki, Motohiro; Tomura, Michio

2015-09-01

Flow cytometric analysis with multicolor fluoroprobes is an essential method for detecting biological signatures of cells. Here, we present a new full-spectral flow cytometer (spectral-FCM). Unlike conventional flow cytometer, this spectral-FCM acquires the emitted fluorescence for all probes across the full-spectrum from each cell with 32 channels sequential PMT unit after dispersion with prism, and extracts the signals of each fluoroprobe based on the spectral shape of each fluoroprobe using unique algorithm in high speed, high sensitive, accurate, automatic and real-time. The spectral-FCM detects the continuous changes in emission spectra from green to red of the photoconvertible protein, KikGR with high-spectral resolution and separates spectrally-adjacent fluoroprobes, such as FITC (Emission peak (Em) 519 nm) and EGFP (Em 507 nm). Moreover, the spectral-FCM can measure and subtract autofluorescence of each cell providing increased signal-to-noise ratios and improved resolution of dim samples, which leads to a transformative technology for investigation of single cell state and function. These advances make it possible to perform 11-color fluorescence analysis to visualize movement of multilinage immune cells by using KikGR-expressing mice. Thus, the novel spectral flow cytometry improves the combinational use of spectrally-adjacent various FPs and multicolor fluorochromes in metabolically active cell for the investigation of not only the immune system but also other research and clinical fields of use. © 2015 International Society for Advancement of Cytometry.

Exploiting physical constraints for multi-spectral exo-planet detection

NASA Astrophysics Data System (ADS)

Thiébaut, Éric; Devaney, Nicholas; Langlois, Maud; Hanley, Kenneth

2016-07-01

We derive a physical model of the on-axis PSF for a high contrast imaging system such as GPI or SPHERE. This model is based on a multi-spectral Taylor series expansion of the diffraction pattern and predicts that the speckles should be a combination of spatial modes with deterministic chromatic magnification and weighting. We propose to remove most of the residuals by fitting this model on a set of images at multiple wavelengths and times. On simulated data, we demonstrate that our approach achieves very good speckle suppression without additional heuristic parameters. The residual speckles1, 2 set the most serious limitation in the detection of exo-planets in high contrast coronographic images provided by instruments such as SPHERE3 at the VLT, GPI4, 5 at Gemini, or SCExAO6 at Subaru. A number of post-processing methods have been proposed to remove as much as possible of the residual speckles while preserving the signal from the planets. These methods exploit the fact that the speckles and the planetary signal have different temporal and spectral behaviors. Some methods like LOCI7 are based on angular differential imaging8 (ADI), spectral differential imaging9, 10 (SDI), or on a combination of ADI and SDI.11 Instead of working on image differences, we propose to tackle the exo-planet detection as an inverse problem where a model of the residual speckles is fit on the set of multi-spectral images and, possibly, multiple exposures. In order to reduce the number of degrees of freedom, we impose specific constraints on the spatio-spectral distribution of stellar speckles. These constraints are deduced from a multi-spectral Taylor series expansion of the diffraction pattern for an on-axis source which implies that the speckles are a combination of spatial modes with deterministic chromatic magnification and weighting. Using simulated data, the efficiency of speckle removal by fitting the proposed multi-spectral model is compared to the result of using an approximation

Hyper-spectral imaging in scanning-confocal-fluorescence microscopy using a novel broadband diffractive optic

NASA Astrophysics Data System (ADS)

Wang, Peng; Ebeling, Carl G.; Gerton, Jordan; Menon, Rajesh

In this paper, we demonstrate hyper-spectral imaging of fluorescent microspheres in a scanning-confocal-fluorescence microscope by spatially dispersing the spectra using a novel broadband diffractive optic, and applying a nonlinear optimization technique to extract the full-incident spectra. This broadband diffractive optic has a designed optical efficiency of over 90% across the entire visible spectrum. We used this technique to create two-color images of two fluorophores and also extracted their emission spectra with good fidelity. This method can be extended to image both spatially and spectrally overlapping fluorescent samples. Full control in the number of emission spectra and the feasibility of enhanced imaging speed are demonstrated as well.

Denaturing of single electrospun fibrinogen fibers studied by deep ultraviolet fluorescence microscopy.

PubMed

Kim, Jeongyong; Song, Hugeun; Park, Inho; Carlisle, Christine R; Bonin, Keith; Guthold, Martin

2011-03-01

Deep ultraviolet (DUV) microscopy is a fluorescence microscopy technique to image unlabeled proteins via the native fluorescence of some of their amino acids. We constructed a DUV fluorescence microscope, capable of 280 nm wavelength excitation by modifying an inverted optical microscope. Moreover, we integrated a nanomanipulator-controlled micropipette into this instrument for precise delivery of picoliter amounts of fluid to selected regions of the sample. In proof-of-principle experiments, we used this instrument to study, in situ, the effect of a denaturing agent on the autofluorescence intensity of single, unlabeled, electrospun fibrinogen nanofibers. Autofluorescence emission from the nanofibers was excited at 280 nm and detected at ∼350 nm. A denaturant solution was discretely applied to small, select sections of the nanofibers and a clear local reduction in autofluorescence intensity was observed. This reduction is attributed to the dissolution of the fibers and the unfolding of proteins in the fibers. Copyright © 2010 Wiley-Liss, Inc.

Multimodal Spectral Imaging of Cells Using a Transmission Diffraction Grating on a Light Microscope

PubMed Central

Isailovic, Dragan; Xu, Yang; Copus, Tyler; Saraswat, Suraj; Nauli, Surya M.

2011-01-01

A multimodal methodology for spectral imaging of cells is presented. The spectral imaging setup uses a transmission diffraction grating on a light microscope to concurrently record spectral images of cells and cellular organelles by fluorescence, darkfield, brightfield, and differential interference contrast (DIC) spectral microscopy. Initially, the setup was applied for fluorescence spectral imaging of yeast and mammalian cells labeled with multiple fluorophores. Fluorescence signals originating from fluorescently labeled biomolecules in cells were collected through triple or single filter cubes, separated by the grating, and imaged using a charge-coupled device (CCD) camera. Cellular components such as nuclei, cytoskeleton, and mitochondria were spatially separated by the fluorescence spectra of the fluorophores present in them, providing detailed multi-colored spectral images of cells. Additionally, the grating-based spectral microscope enabled measurement of scattering and absorption spectra of unlabeled cells and stained tissue sections using darkfield and brightfield or DIC spectral microscopy, respectively. The presented spectral imaging methodology provides a readily affordable approach for multimodal spectral characterization of biological cells and other specimens. PMID:21639978

Multi-spectral imaging of oxygen saturation

NASA Astrophysics Data System (ADS)

Savelieva, Tatiana A.; Stratonnikov, Aleksander A.; Loschenov, Victor B.

2008-06-01

The system of multi-spectral imaging of oxygen saturation is an instrument that can record both spectral and spatial information about a sample. In this project, the spectral imaging technique is used for monitoring of oxygen saturation of hemoglobin in human tissues. This system can be used for monitoring spatial distribution of oxygen saturation in photodynamic therapy, surgery or sports medicine. Diffuse reflectance spectroscopy in the visible range is an effective and extensively used technique for the non-invasive study and characterization of various biological tissues. In this article, a short review of modeling techniques being currently in use for diffuse reflection from semi-infinite turbid media is presented. A simple and practical model for use with a real-time imaging system is proposed. This model is based on linear approximation of the dependence of the diffuse reflectance coefficient on relation between absorbance and reduced scattering coefficient. This dependence was obtained with the Monte Carlo simulation of photon propagation in turbid media. Spectra of the oxygenated and deoxygenated forms of hemoglobin differ mostly in the red area (520 - 600 nm) and have several characteristic points there. Thus four band-pass filters were used for multi-spectral imaging. After having measured the reflectance, the data obtained are used for fitting the concentration of oxygenated and free hemoglobin, and hemoglobin oxygen saturation.

Ultrafast optical transistor and router of multi-order fluorescence and spontaneous parametric four-wave mixing in Pr³⁺:YSO.

PubMed

Wen, Feng; Ali, Imran; Hasan, Abdulkhaleq; Li, Changbiao; Tang, Haijun; Zhang, Yufei; Zhang, Yanpeng

2015-10-15

We study the realization of an optical transistor (switch and amplifier) and router in multi-order fluorescence (FL) and spontaneous parametric four-wave mixing (SP-FWM). We estimate that the switching speed is about 15 ns. The router action results from the Autler-Townes splitting in spectral or time domain. The switch and amplifier are realized by dressing suppression and enhancement in FL and SP-FWM. The optical transistor and router can be controlled by multi-parameters (i.e., power, detuning, or polarization).

Time- and spectrally resolved characteristics of flavin fluorescence in U87MG cancer cells in culture

NASA Astrophysics Data System (ADS)

Horilova, Julia; Cunderlikova, Beata; Marcek Chorvatova, Alzbeta

2015-05-01

Early detection of cancer is crucial for the successful diagnostics of its presence and its subsequent treatment. To improve cancer detection, we tested the progressive multimodal optical imaging of U87MG cells in culture. A combination of steady-state spectroscopic methods with the time-resolved approach provides a new insight into the native metabolism when focused on endogenous tissue fluorescence. In this contribution, we evaluated the metabolic state of living U87MG cancer cells in culture by means of endogenous flavin fluorescence. Confocal microscopy and time-resolved fluorescence imaging were employed to gather spectrally and time-resolved images of the flavin fluorescence. We observed that flavin fluorescence in U87MG cells was predominantly localized outside the cell nucleus in mitochondria, while exhibiting a spectral maximum under 500 nm and fluorescence lifetimes under 1.4 ns, suggesting the presence of bound flavins. In some cells, flavin fluorescence was also detected inside the cell nuclei in the nucleoli, exhibiting longer fluorescence lifetimes and a red-shifted spectral maximum, pointing to the presence of free flavin. Extra-nuclear flavin fluorescence was diminished by 2-deoxyglucose, but failed to increase with 2,4-dinitrophenol, the uncoupler of oxidative phosphorylation, indicating that the cells use glycolysis, rather than oxidative phosphorylation for functioning. These gathered data are the first step toward monitoring the metabolic state of U87MG cancer cells.

Fluorescence spectral analysis of resinite macerals from coals of the Hanna Formation, Wyoming, U.S.A.

USGS Publications Warehouse

Teerman, S.C.; Crelling, J.C.; Glass, G.B.

1987-01-01

Flourescence spectral analysis indicates that resinite macerals from Tertiary Hanna Formation coals (Hanna Coal Field, southcentral Wyoming, U.S.A.) can be separated into five distinct groups. The first resinite group fluoresces a a medium green (in blue light); its average spectral maximum occurs at or below 440 mm with a red/green quotient of 0.22. The second resinite group fluoresces yellow-green with an average spectral maximum of 500 nm and a red/green quotient of 0.53. The third resinite group displays a yellow fluorescence having an average spectral maximum of 580 nm and a red/green quotient of 0.86. The fourth resinite group fluorescence orange-brown having an average spectral maximum of 610 nm and a red/green quotient of 1.20. These four groups mostly occur as primary globular resinites exhibiting scratches and fractures, indicating that they are brittle, solid substances. Primary cell-filling and secondary fracture-filling resinites also occur in these four groups. The fifth group only occurs as a secondary void-filling material and lacks evidence of br of brittle properties. It fluoresces a reddish-brown, has a spectral maximum at 690 nm, and a red/green quotient of 1.54. The fifth group has properties resembling exsudatinite. The five resinite groups can be separated on the basis of their nine spectral properties alone, without qualitative petrographic interpretation. The relative quantities of the five resinite groups vary among Hanna Formation coals. The origins of these five resinite groups are probably related to their botanical properties and pre- and post-depossitional conditions. Overall, Hanna Formation resinites have petrographic characteristics similar to other North American resinites; however, only four resinite groups have been distinguished in in certain coals from Utah and New Mexico (U.S.A.), and western Canada. ?? 1987.

A DETAILED FAR-ULTRAVIOLET SPECTRAL ATLAS OF MAIN-SEQUENCE B STARS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smith, Myron A.

2010-02-01

We have constructed a detailed spectral atlas covering the wavelength region 930-1225 A for 10 sharp-lined B0-B9 stars near the main sequence. Most of the spectra we assembled are from the archives of the Far Ultraviolet Spectroscopic Explorer satellite, but for nine stars, wavelength coverage above 1188 A was taken from high-resolution International Ultraviolet Explorer or echelle Hubble Space Telescope/Space Telescope Imaging Spectrograph spectra. To represent the tenth star at type B0.2 V, we used the Copernicus atlas of {tau} Sco. We made extensive line identifications in the region 949-1225 A of all atomic features having published oscillator strengths atmore » types B0, B2, and B8. These are provided as a supplementary data product-hence the term detailed atlas. Our list of found features totals 2288, 1612, and 2469 lines, respectively. We were able to identify 92%, 98%, and 98% of these features with known atomic transitions with varying degrees of certainty in these spectra. The remaining lines do not have published oscillator strengths. Photospheric lines account for 94%, 87%, and 91%, respectively, of all our identifications, with the remainder being due to interstellar (usually molecular H{sub 2}) lines. We also discuss the numbers of lines with respect to the distributions of various ions for these three most studied spectral subtypes. A table is also given of 162 least blended lines that can be used as possible diagnostics of physical conditions in B star atmospheres.« less

Spectral Neugebauer-based color halftone prediction model accounting for paper fluorescence.

PubMed

Hersch, Roger David

2014-08-20

We present a spectral model for predicting the fluorescent emission and the total reflectance of color halftones printed on optically brightened paper. By relying on extended Neugebauer models, the proposed model accounts for the attenuation by the ink halftones of both the incident exciting light in the UV wavelength range and the emerging fluorescent emission in the visible wavelength range. The total reflectance is predicted by adding the predicted fluorescent emission relative to the incident light and the pure reflectance predicted with an ink-spreading enhanced Yule-Nielsen modified Neugebauer reflectance prediction model. The predicted fluorescent emission spectrum as a function of the amounts of cyan, magenta, and yellow inks is very accurate. It can be useful to paper and ink manufacturers who would like to study in detail the contribution of the fluorescent brighteners and the attenuation of the fluorescent emission by ink halftones.

Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jin, Qiaoling; Vogt, Stefan; Lai, Barry

Rapidly-frozen hydrated (cryopreserved) specimens combined with cryo-scanning x-ray fluorescence microscopy provide an ideal approach for investigating elemental distributions in biological cells and tissues. However, because cryopreservation does not deactivate potentially infectious agents associated with Risk Group 2 biological materials, one must be concerned with contamination of expensive and complicated cryogenic x-ray microscopes when working with such materials. We employed ultraviolet germicidal irradiation to decontaminate previously cryopreserved cells under liquid nitrogen, and then investigated its effects on elemental distributions under both frozen hydrated and freeze dried states with x-ray fluorescence microscopy. We show that the contents and distributions of most biologicallymore » important elements remain nearly unchanged when compared with non-ultraviolet-irradiated counterparts, even after multiple cycles of ultraviolet germicidal irradiation and cryogenic x-ray imaging. This provides a potential pathway for rendering Risk Group 2 biological materials safe for handling in multiuser cryogenic x-ray microscopes without affecting the fidelity of the results.« less

Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis

DOE PAGES

Jin, Qiaoling; Vogt, Stefan; Lai, Barry; ...

2015-02-23

Rapidly-frozen hydrated (cryopreserved) specimens combined with cryo-scanning x-ray fluorescence microscopy provide an ideal approach for investigating elemental distributions in biological cells and tissues. However, because cryopreservation does not deactivate potentially infectious agents associated with Risk Group 2 biological materials, one must be concerned with contamination of expensive and complicated cryogenic x-ray microscopes when working with such materials. We employed ultraviolet germicidal irradiation to decontaminate previously cryopreserved cells under liquid nitrogen, and then investigated its effects on elemental distributions under both frozen hydrated and freeze dried states with x-ray fluorescence microscopy. We show that the contents and distributions of most biologicallymore » important elements remain nearly unchanged when compared with non-ultraviolet-irradiated counterparts, even after multiple cycles of ultraviolet germicidal irradiation and cryogenic x-ray imaging. This provides a potential pathway for rendering Risk Group 2 biological materials safe for handling in multiuser cryogenic x-ray microscopes without affecting the fidelity of the results.« less

Wavelet packets for multi- and hyper-spectral imagery

NASA Astrophysics Data System (ADS)

Benedetto, J. J.; Czaja, W.; Ehler, M.; Flake, C.; Hirn, M.

2010-01-01

State of the art dimension reduction and classification schemes in multi- and hyper-spectral imaging rely primarily on the information contained in the spectral component. To better capture the joint spatial and spectral data distribution we combine the Wavelet Packet Transform with the linear dimension reduction method of Principal Component Analysis. Each spectral band is decomposed by means of the Wavelet Packet Transform and we consider a joint entropy across all the spectral bands as a tool to exploit the spatial information. Dimension reduction is then applied to the Wavelet Packets coefficients. We present examples of this technique for hyper-spectral satellite imaging. We also investigate the role of various shrinkage techniques to model non-linearity in our approach.

Laser-induced fluorescence imaging of subsurface tissue structures with a volume holographic spatial-spectral imaging system.

PubMed

Luo, Yuan; Gelsinger-Austin, Paul J; Watson, Jonathan M; Barbastathis, George; Barton, Jennifer K; Kostuk, Raymond K

2008-09-15

A three-dimensional imaging system incorporating multiplexed holographic gratings to visualize fluorescence tissue structures is presented. Holographic gratings formed in volume recording materials such as a phenanthrenquinone poly(methyl methacrylate) photopolymer have narrowband angular and spectral transmittance filtering properties that enable obtaining spatial-spectral information within an object. We demonstrate this imaging system's ability to obtain multiple depth-resolved fluorescence images simultaneously.

Voigt spectral profiles in two-photon resonance fluorescence

DOE Office of Scientific and Technical Information (OSTI.GOV)

Alexanian, Moorad; Bose, Subir K.; Department of Physics, University of Central Florida, Orlando, Florida 32816

2007-11-15

A recent work on two-photon fluorescence is extended by considering the pump field to be a coherent state, which represents a laser field operating well above threshold. The dynamical conditions are investigated under which the two-photon spectrum gives rise, in addition to a Lorentzian line shape at the pump frequency, to two Voigt spectral sideband profiles. Additional conditions are found under which the Voigt profile behaves like either a Gaussian or a Lorentzian line shape.

[Near ultraviolet absorption spectral properties of chromophoric dissolved organic matter in the north area of Yellow Sea].

PubMed

Wang, Lin; Zhao, Dong-Zhi; Yang, Jian-Hong; Chen, Yan-Long

2010-12-01

Chromophoric dissolved organic matter (CDOM) near ultraviolet absorption spectra contains CDOM molecular structure, composition and other important physical and chemical information. Based on the measured data of CDOM absorption coefficient in March 2009 in the north area of Yellow Sea, the present paper analyzed near ultraviolet absorption spectral properties of CDOM. The results showed that due to the impact of near-shore terrigenous input, the composition of CDOM is quite different in the north area of Yellow Sea, and this area is a typical case II water; fitted slope with specific range of spectral band and absorption coefficient at specific band can indicate the relative size of CDOM molecular weight, correlation between spectral slope of the Sg,275-300), Sg,300-350, Sg,350-400 and Sg,250-275 and the relative size of CDOM molecular weight indicative parameter M increases in turn and the highest is up to 0.95. Correlation between a(g)(lambda) and M value increases gradually with the increase in wavelength, and the highest is up to 0.92 at 400 nm; being correlated or not between spectral slope and absorption coefficient is decided by the fitting-band wavelength range for the spectra slope and the wavelength for absorption coefficient. Correlation between Sg,275-300 and a(g)(400) is the largest, up to 0.87.

Physical Interpretation of the Correlation Between Multi-Angle Spectral Data and Canopy Height

NASA Technical Reports Server (NTRS)

Schull, M. A.; Ganguly, S.; Samanta, A.; Huang, D.; Shabanov, N. V.; Jenkins, J. P.; Chiu, J. C.; Marshak, A.; Blair, J. B.; Myneni, R. B.;

Airborne Multi-Spectral Minefield Survey

DTIC Science & Technology

2005-05-01

Swedish Defence Research Agency), GEOSPACE (Austria), GTD ( Ingenieria de Sistemas y Software Industrial, Spain), IMEC (Ineruniversity MicroElectronic...RTO-MP-SET-092 18 - 1 UNCLASSIFIED/UNLIMITED UNCLASSIFIED/UNLIMITED Airborne Multi-Spectral Minefield Survey Dirk-Jan de Lange, Eric den...actions is the severe lack of baseline information. To respond to this in a rapid way, cost-efficient data acquisition methods are a key issue. de

[Assessment of mitochondrial metabolic oxidative state in living cardiomyocytes with spectrally-resolved fluorescence lifetime spectroscopy of NAD(P)H].

PubMed

Cheng, Ying; Ren, Mingming; Niu, Yanyan; Qiao, Jianhua; Aneba, S; Chorvat, D; Chorvatova, A

2009-12-01

The primary function of cardiac mitochondria is the production of ATP to support heart contraction. Examination of the mitochondrial redox state is therefore crucially important to sensitively detect early signs of mitochondrial function in pathophysiological conditions, such as ischemia, diabetes and heart failure. We study fingerprinting of mitochondrial metabolic oxidative state in living cardiomyocytes with spectrally-resolved fluorescence lifetime spectroscopy of NAD(P)H, the principal electron donor in mitochondrial respiration responsible for vital ATP supply. Here NAD(P)H is studied as a marker for non-invasive fluorescent probing of the mitochondrial function. NAD(P) H fluorescence is recorded in cardiac cells following excitation with 375nm UV-light and detection by spectrally-resolved time-correlated single photon counting (TCSPC), based on the simultaneous measurement of the fluorescence spectra and fluorescence lifetimes. Modulation of NADH production and/or mitochondrial respiration is tested to study dynamic characteristics of NAD(P) H fluorescence decay. Our results show that at least a 3-exponential decay model, with 0.4-0.7ns, 1.2-1.9ns and 8.0-13. Ons lifetime pools is necessary to describe cardiomyocyte autofluorescence (AF) within 420-560nm spectral range. Increased mitochondrial NADH production by ketone bodies enhanced the fluorescence intensity, without significant change in fluorescent lifetimes. Rotenone, the inhibitor of Complex I of the mitochondrial respiratory chain, increased AF intensity and shortened the average fluorescence lifetime. Dinitrophenol (DNP), an uncoupling agent of the mitochondrial oxidative phosphorylation, lowered AF intensity, broadened the spectral shoulder at 520 nm and increased the average fluorescence lifetime. These effects are comparable to the study of NADH fluorescence decay in vitro. In the present contribution we demonstrated that spectrally-resolved fluorescence lifetime technique provides promising new

[Study of the Detecting System of CH4 and SO2 Based on Spectral Absorption Method and UV Fluorescence Method].

PubMed

Wang, Shu-tao; Wang, Zhi-fang; Liu, Ming-hua; Wei, Meng; Chen, Dong-ying; Wang, Xing-long

2016-01-01

According to the spectral absorption characteristics of polluting gases and fluorescence characteristics, a time-division multiplexing detection system is designed. Through this system we can detect Methane (CH4) and sulfur dioxide (SO2) by using spectral absorption method and the SO2 can be detected by using UV fluorescence method. The system consists of four parts: a combination of a light source which could be switched, the common optical path, the air chamber and the signal processing section. The spectral absorption characteristics and fluorescence characteristics are measured first. Then the experiment of detecting CH4 and SO2 through spectral absorption method and the experiment of detecting SO2 through UV fluorescence method are conducted, respectively. Through measuring characteristics of spectral absorption and fluorescence, we get excitation wavelengths of SO2 and CH4 measured by spectral absorption method at the absorption peak are 280 nm and 1.64 μm, respectively, and the optimal excitation wavelength of SO2 measured by UV fluorescence method is 220 nm. we acquire the linear relation between the concentration of CH4 and relative intensity and the linear relation between the concentration of SO2 and output voltage after conducting the experiment of spectral absorption method, and the linearity are 98.7%, 99.2% respectively. Through the experiment of UV fluorescence method we acquire that the relation between the concentration of SO2 and the voltage is linear, and the linearity is 99.5%. Research shows that the system is able to be applied to detect the polluted gas by absorption spectrum method and UV fluorescence method. Combing these two measurement methods decreases the costing and the volume, and this system can also be used to measure the other gases. Such system has a certain value of application.

Optical Sensing of Ecosystem Carbon Fluxes Combining Spectral Reflectance Indices with Solar Induced Fluorescence

NASA Astrophysics Data System (ADS)

Huemmrich, K. F.; Middleton, E.; Corp, L. A.; Campbell, P. K.; Kustas, W. P.

2014-12-01

Optical sampling of spectral reflectance and solar induced fluorescence provide information on the physiological status of vegetation that can be used to infer stress responses and estimates of production. Multiple repeated observations are required to observe the effects of changing environmental conditions on vegetation. This study examines the use of optical signals to determine inputs to a light use efficiency (LUE) model describing productivity of a cornfield where repeated observations of carbon flux, spectral reflectance and fluorescence were collected. Data were collected at the Optimizing Production Inputs for Economic and Environmental Enhancement (OPE3) fields (39.03°N, 76.85°W) at USDA Beltsville Agricultural Research Center. Agricultural Research Service researchers measured CO2 fluxes using eddy covariance methods throughout the growing season. Optical measurements were made from the nearby tower supporting the NASA FUSION sensors. The sensor system consists of two dual channel, upward and downward looking, spectrometers used to simultaneously collect high spectral resolution measurements of reflected and fluoresced light from vegetation canopies. Estimates of chlorophyll fluorescence, combined with measures of vegetation pigment content and the Photosynthetic Reflectance Index (PRI) derived from the spectral reflectance are compared with CO2 fluxes over diurnal periods for multiple days. PRI detects changes in Xanthophyll cycle pigments using reflectance at 531 nm compared to a reference band at 570 nm. The relationships among the different optical measurements indicate that they are providing different types of information on the vegetation and that combinations of these measurements provide improved retrievals of CO2 fluxes than any index alone.

Optical Sensing of Ecosystem Carbon Fluxes Combining Spectral Reflectance Indices with Solar Induced Fluorescence

NASA Astrophysics Data System (ADS)

Huemmrich, K. F.; Corp, L.; Campbell, P. K.; Cook, B. D.; Middleton, E.; Cheng, Y.; Zhang, Q.; Russ, A.; Kustas, W. P.

2013-12-01

Optical sampling of spectral reflectance and solar induced fluorescence provide information on the physiological status of vegetation that can be used to infer stress responses and estimates of production. Multiple repeated observations can observe the effects of changing environmental conditions on vegetation. This study examines the use of optical signals to determine inputs to a light use efficiency (LUE) model describing productivity of a cornfield where repeated observations of carbon flux, spectral reflectance and fluorescence were collected. Data were collected at the Optimizing Production Inputs for Economic and Environmental Enhancement (OPE3) fields (39.03°N, 76.85°W) at USDA Beltsville Agricultural Research Center. Agricultural Research Service researchers measured CO2 fluxes using eddy covariance methods throughout the growing season. Optical measurements were made from the nearby tower supporting the NASA FUSION sensors. This sensor system consists of two dual channel, upward and downward looking, spectrometers used to simultaneously collect high spectral resolution measurements of reflected and fluoresced light from vegetation canopies. Estimates of chlorophyll fluorescence, combined with measures of vegetation pigment content and the Photosynthetic Reflectance Index (PRI) derived from the spectral reflectance are compared with CO2 fluxes over diurnal periods for multiple days. PRI detects changes in Xanthophyll cycle pigments using reflectance at 531 nm compared to a reference band at 570 nm. The relationships among the different optical measurements indicate that they are providing different types of information on the vegetation and that combinations of these measurements provide improved retrievals of CO2 fluxes than any index alone.

Spectral classification with the International Ultraviolet Explorer: An atlas of B-type spectra

NASA Technical Reports Server (NTRS)

Rountree, Janet; Sonneborn, George

1993-01-01

New criteria for the spectral classification of B stars in the ultraviolet show that photospheric absorption lines in the 1200-1900A wavelength region can be used to classify the spectra of B-type dwarfs, subgiants, and giants on a 2-D system consistent with the optical MK system. This atlas illustrates a large number of such spectra at the scale used for classification. These spectra provide a dense matrix of standard stars, and also show the effects of rapid stellar rotation and stellar winds on the spectra and their classification. The observational material consists of high-dispersion spectra from the International Ultraviolet Explorer archives, resampled to a resolution of 0.25 A, uniformly normalized, and plotted at 10 A/cm. The atlas should be useful for the classification of other IUE high-dispersion spectra, especially for stars that have not been observed in the optical.

Ultraviolet-visible and fluorescence spectroscopy can be used as a diagnostic tool for gamma irradiation detection in vivo.

PubMed

K-Abdelhalim, Mohamed Anwar; Moussa, Sherif A-Abdelmottaleb

2016-09-01

The spectroscopic properties can indicate important features about the nature and severity of the disease. However, no earlier studies have been used the spectroscopic properties as a diagnostic tool for radiation detection. This study was aimed to use ultraviolet-visible and fluorescence spectroscopy as a diagnostic tool for gamma irradiation detection in rats in vivo. Adult male rats were exposed to 25, 50, 75 and 100 Gray as single dose, using Cobalt-60 (Co-60) source with a dose rate of 0.883 centi Gray/sec (cGy/s). Ultraviolet and fluorescence spectroscopy of rat's blood serum were measured. After gamma irradiation of rats in vivo, the blood serum absorbance peaks for 25, 50, 75 and 100 Gray (Gy) decreased and shifted towards the ultra violet wavelength. A maximal change in fluorescence intensity of blood serum at 350 nm was obtained when exciting light at 194 nm after irradiation. The fluorescence intensity also decreased with the dose. The highest radiation gamma dose might be accompanied with the highest oxidative stress. This study suggests that at the above mentioned gamma radiation doses, the blood is highly fragmented; with low aggregation at 25 Gy and with high aggregation at 50-100 Gy.

Spectral and Fluorescent Studies of the Interaction of an Anionic Oxacarbocyanine Dye with Bovine Serum Albumin

NASA Astrophysics Data System (ADS)

Pronkin, P. G.; Tatikolov, A. S.

2017-01-01

The influence of the formation of noncovalent intermolecular complexes with bovine serum albumin (BSA) on the spectral and fluorescent properties of the anionic oxacarbocyanine dye 3,3'-di-(γ-sulfopropyl)-5,5'-diphenyl-9-ethyloxacarbocyanine betaine (OCC) was studied. Binding of OCC to BSA increased significantly the dye fluorescence. Changes in the absorption and fluorescence spectra of OCC upon interaction with BSA argued in favor of a shift of the dye cis-trans equilibrium in the complex. The effects of adding albumin-denaturing compounds (urea, sodium dodecyl sulfate) on the spectral and fluorescent properties of the dye in the OCC-BSA complex were studied. It was concluded that OCC can act as a probe for albumins and can be used to study protein denaturing.

Degradation of microbial fluorescence biosignatures by solar ultraviolet radiation on Mars

NASA Astrophysics Data System (ADS)

Dartnell, Lewis R.; Patel, Manish R.

2014-04-01

Recent and proposed robotic missions to Mars are equipped with implements to expose or excavate fresh material from beneath the immediate surface. Once brought into the open, any organic molecules or potential biosignatures of present or past life will be exposed to the unfiltered solar ultraviolet (UV) radiation and face photolytic degradation over short time courses. The key question, then, is what is the window of opportunity for detection of recently exposed samples during robotic operations? Detection of autofluorescence has been proposed as a simple method for surveying or triaging samples for organic molecules. Using a Mars simulation chamber we conduct UV exposures on thin frozen layers of two model microorganisms, the radiation-resistant polyextremophile Deinococcus radiodurans and the cyanobacterium Synechocystis sp. PCC 6803. Excitation-emission matrices (EEMs) are generated of the full fluorescence response to quantify the change in signal of different cellular fluorophores over Martian equivalent time. Fluorescence of Deinococcus cells, protected by a high concentration of carotenoid pigments, was found to be relatively stable over 32 h of Martian UV irradiation, with around 90% of the initial signal remaining. By comparison, fluorescence from protein-bound tryptophan in Synechocystis is much more sensitive to UV photodegradation, declining to 50% after 64 h exposure. The signal most readily degraded by UV irradiation is fluorescence of the photosynthetic pigments - diminished to only 35% after 64 h. This sensitivity may be expected as the biological function of chlorophyll and phycocyanin is to optimize the harvesting of light energy and so they are readily photobleached. A significant increase in a ~450 nm emission feature is interpreted as accumulation of fluorescent cellular degradation products from photolysis. Accounting for diurnal variation in Martian sunlight, this study calculates that frozen cellular biosignatures would remain detectable by

Can the circadian system of a diurnal and a nocturnal rodent entrain to ultraviolet light?

PubMed

Hut, R A; Scheper, A; Daan, S

2000-01-01

Spectral measurements of sunlight throughout the day show close correspondence between the timing of above ground activity of the European ground squirrel and the presence of ultraviolet light in the solar spectrum. However, in a standard entrainment experiment ground squirrels show no entrainment to ultraviolet light, while Syrian hamsters do entrain under the same protocol. Presented transmittance spectra for lenses, corneas, and vitreous bodies may explain the different results of the entrainment experiment. We found ultraviolet light transmittance in the colourless hamster lens (50% cut-off at 341 nm), but not in the yellow ground squirrel lens (50% cut-off around 493 nm). Ultraviolet sensitivity in the ground squirrels based upon possible fluorescence mechanisms was not evident. Possible functions of ultraviolet lens filters in diurnal mammals are discussed, and compared with nocturnal mammals and diurnal birds. Species of the latter two groups lack ultraviolet filtering properties of their lenses and their circadian system is known to respond to ultraviolet light, a feature that does not necessarily has to depend on ultraviolet photoreceptors. Although the circadian system of several species responds to ultraviolet light, we argue that the role of ultraviolet light as a natural Zeitgeber is probably limited.

Optimized Multi-Spectral Filter Array Based Imaging of Natural Scenes.

PubMed

Li, Yuqi; Majumder, Aditi; Zhang, Hao; Gopi, M

2018-04-12

Multi-spectral imaging using a camera with more than three channels is an efficient method to acquire and reconstruct spectral data and is used extensively in tasks like object recognition, relighted rendering, and color constancy. Recently developed methods are used to only guide content-dependent filter selection where the set of spectral reflectances to be recovered are known a priori. We present the first content-independent spectral imaging pipeline that allows optimal selection of multiple channels. We also present algorithms for optimal placement of the channels in the color filter array yielding an efficient demosaicing order resulting in accurate spectral recovery of natural reflectance functions. These reflectance functions have the property that their power spectrum statistically exhibits a power-law behavior. Using this property, we propose power-law based error descriptors that are minimized to optimize the imaging pipeline. We extensively verify our models and optimizations using large sets of commercially available wide-band filters to demonstrate the greater accuracy and efficiency of our multi-spectral imaging pipeline over existing methods.

Optimized Multi-Spectral Filter Array Based Imaging of Natural Scenes

PubMed Central

Li, Yuqi; Majumder, Aditi; Zhang, Hao; Gopi, M.

2018-01-01

Multi-spectral imaging using a camera with more than three channels is an efficient method to acquire and reconstruct spectral data and is used extensively in tasks like object recognition, relighted rendering, and color constancy. Recently developed methods are used to only guide content-dependent filter selection where the set of spectral reflectances to be recovered are known a priori. We present the first content-independent spectral imaging pipeline that allows optimal selection of multiple channels. We also present algorithms for optimal placement of the channels in the color filter array yielding an efficient demosaicing order resulting in accurate spectral recovery of natural reflectance functions. These reflectance functions have the property that their power spectrum statistically exhibits a power-law behavior. Using this property, we propose power-law based error descriptors that are minimized to optimize the imaging pipeline. We extensively verify our models and optimizations using large sets of commercially available wide-band filters to demonstrate the greater accuracy and efficiency of our multi-spectral imaging pipeline over existing methods. PMID:29649114

Retinal oxygen saturation evaluation by multi-spectral fundus imaging

NASA Astrophysics Data System (ADS)

Khoobehi, Bahram; Ning, Jinfeng; Puissegur, Elise; Bordeaux, Kimberly; Balasubramanian, Madhusudhanan; Beach, James

2007-03-01

Purpose: To develop a multi-spectral method to measure oxygen saturation of the retina in the human eye. Methods: Five Cynomolgus monkeys with normal eyes were anesthetized with intramuscular ketamine/xylazine and intravenous pentobarbital. Multi-spectral fundus imaging was performed in five monkeys with a commercial fundus camera equipped with a liquid crystal tuned filter in the illumination light path and a 16-bit digital camera. Recording parameters were controlled with software written specifically for the application. Seven images at successively longer oxygen-sensing wavelengths were recorded within 4 seconds. Individual images for each wavelength were captured in less than 100 msec of flash illumination. Slightly misaligned images of separate wavelengths due to slight eye motion were registered and corrected by translational and rotational image registration prior to analysis. Numerical values of relative oxygen saturation of retinal arteries and veins and the underlying tissue in between the artery/vein pairs were evaluated by an algorithm previously described, but which is now corrected for blood volume from averaged pixels (n > 1000). Color saturation maps were constructed by applying the algorithm at each image pixel using a Matlab script. Results: Both the numerical values of relative oxygen saturation and the saturation maps correspond to the physiological condition, that is, in a normal retina, the artery is more saturated than the tissue and the tissue is more saturated than the vein. With the multi-spectral fundus camera and proper registration of the multi-wavelength images, we were able to determine oxygen saturation in the primate retinal structures on a tolerable time scale which is applicable to human subjects. Conclusions: Seven wavelength multi-spectral imagery can be used to measure oxygen saturation in retinal artery, vein, and tissue (microcirculation). This technique is safe and can be used to monitor oxygen uptake in humans. This work

Comparison of spectral radiance responsivity calibration techniques used for backscatter ultraviolet satellite instruments

NASA Astrophysics Data System (ADS)

Kowalewski, M. G.; Janz, S. J.

2015-02-01

Methods of absolute radiometric calibration of backscatter ultraviolet (BUV) satellite instruments are compared as part of an effort to minimize pre-launch calibration uncertainties. An internally illuminated integrating sphere source has been used for the Shuttle Solar BUV, Total Ozone Mapping Spectrometer, Ozone Mapping Instrument, and Global Ozone Monitoring Experiment 2 using standardized procedures traceable to national standards. These sphere-based spectral responsivities agree to within the derived combined standard uncertainty of 1.87% relative to calibrations performed using an external diffuser illuminated by standard irradiance sources, the customary spectral radiance responsivity calibration method for BUV instruments. The combined standard uncertainty for these calibration techniques as implemented at the NASA Goddard Space Flight Center’s Radiometric Calibration and Development Laboratory is shown to less than 2% at 250 nm when using a single traceable calibration standard.

Clinical measurements analysis of multi-spectral photoplethysmograph biosensors

NASA Astrophysics Data System (ADS)

Asare, Lasma; Kviesis-Kipge, Edgars; Spigulis, Janis

2014-05-01

The developed portable multi-spectral photoplethysmograph (MS-PPG) optical biosensor device, intended for analysis of peripheral blood volume pulsations at different vascular depths, has been clinically verified. Multi-spectral monitoring was performed by means of a four - wavelengths (454 nm, 519 nm, 632 nm and 888 nm) light emitted diodes and photodiode with multi-channel signal output processing. Two such sensors can be operated in parallel and imposed on the patient's skin. The clinical measurements confirmed ability to detect PPG signals at four wavelengths simultaneously and to record temporal differences in the signal shapes (corresponding to different penetration depths) in normal and pathological skin. This study analyzed wavelengths relations between systole and diastole peak difference at various tissue depths in normal and pathological skin. The difference between parameters of healthy and pathological skin at various skin depths could be explain by oxy- and deoxyhemoglobin dominance at different wavelengths operated in sensor. The proposed methodology and potential clinical applications in dermatology for skin assessment are discussed.

High performance multi-spectral interrogation for surface plasmon resonance imaging sensors.

PubMed

Sereda, A; Moreau, J; Canva, M; Maillart, E

2014-04-15

Surface plasmon resonance (SPR) sensing has proven to be a valuable tool in the field of surface interactions characterization, especially for biomedical applications where label-free techniques are of particular interest. In order to approach the theoretical resolution limit, most SPR-based systems have turned to either angular or spectral interrogation modes, which both offer very accurate real-time measurements, but at the expense of the 2-dimensional imaging capability, therefore decreasing the data throughput. In this article, we show numerically and experimentally how to combine the multi-spectral interrogation technique with 2D-imaging, while finding an optimum in terms of resolution, accuracy, acquisition speed and reduction in data dispersion with respect to the classical reflectivity interrogation mode. This multi-spectral interrogation methodology is based on a robust five parameter fitting of the spectral reflectivity curve which enables monitoring of the reflectivity spectral shift with a resolution of the order of ten picometers, and using only five wavelength measurements per point. In fine, such multi-spectral based plasmonic imaging system allows biomolecular interaction monitoring in a linear regime independently of variations of buffer optical index, which is illustrated on a DNA-DNA model case. © 2013 Elsevier B.V. All rights reserved.

Room temperature spectrally resolved single-molecule spectroscopy reveals new spectral forms and photophysical versatility of aequorea green fluorescent protein variants.

PubMed

Blum, Christian; Meixner, Alfred J; Subramaniam, Vinod

2004-12-01

It is known from ensemble spectroscopy at cryogenic temperatures that variants of the Aequorea green fluorescent protein (GFP) occur in interconvertible spectroscopically distinct forms which are obscured in ensemble room temperature spectroscopy. By analyzing the fluorescence of the GFP variants EYFP and EGFP by spectrally resolved single-molecule spectroscopy we were able to observe spectroscopically different forms of the proteins and to dynamically monitor transitions between these forms at room temperature. In addition to the predominant EYFP B-form we have observed the blue-shifted I-form thus far only seen at cryogenic temperatures and have followed transitions between these forms. Further we have identified for EYFP and for EGFP three more, so far unknown, forms with red-shifted fluorescence. Transitions between the predominant forms and the red-shifted forms show a dark time which indicates the existence of a nonfluorescent intermediate. The spectral position of the newly-identified red-shifted forms and their formation via a nonfluorescent intermediate hint that these states may account for the possible photoactivation observed in bulk experiments. The comparison of the single-protein spectra of the red-shifted EYFP and EGFP forms with single-molecule fluorescence spectra of DsRed suggest that these new forms possibly originate from an extended chromophoric pi-system analogous to the DsRed chromophore.

Time-resolved spectral studies of blue-green fluorescence of artichoke (Cynara cardunculus L. Var. Scolymus) leaves: identification of chlorogenic acid as one of the major fluorophores and age-mediated changes.

PubMed

Morales, Fermín; Cartelat, Aurélie; Alvarez-Fernández, Ana; Moya, Ismael; Cerovic, Zoran G

2005-12-14

Synchrotron radiation and the time-correlated single-photon counting technique were used to investigate the spectral and time-resolved characteristics of blue-green fluorescence (BGF) of artichoke leaves. Leaves emitted BGF under ultraviolet (UV) excitation; the abaxial side was much more fluorescent than the adaxial side, and in both cases, the youngest leaves were much more fluorescent than the oldest ones. The BGF of artichoke leaves was dominated by the presence of hydroxycinnamic acids. A decrease in the percentage of BGF attributable to the very short kinetic component (from 42 to 20%), in the shape of the BGF excitation spectra, and chlorogenic acid concentrations indicate that there is a loss of hydroxycinnamic acid with leaf age. Studies on excitation, emission, and synchronized fluorescence spectra of leaves and trichomes and chlorogenic acid contents indicate that chlorogenic acid is one of the main blue-green fluorophores in artichoke leaves. Results of the present study indicate that 20-42% (i.e., the very short kinetic component) of the overall BGF is emitted by chlorogenic acid. Time-resolved BGF measurements could be a means to extract information on chlorogenic acid fluorescence from the overall leaf BGF.

Spectral analysis of natural solar ultraviolet B to promote synthesis of vitamin D

NASA Astrophysics Data System (ADS)

Hung, Min-Wei; Lin, Yu-Hsuan; Chang, Han-Chao; Huang, Kuo-Cheng

2016-10-01

This paper presents a spectral analysis system for the measurement of solar ultraviolet B over long durations. The proposed system provides high resolution at low cost in a highly robust and flexible format. We obtained information pertaining to the absolute irradiance of sunlight in a fixed location with the aim of identifying the best period in which to seek exposure to the sun with regard to maximizing the synthesis of vitamin D while minimizing damage to the skin. This study also provides a means of establishing a database for the development of healthy lamp technology.

Canadian Thesis Abstracts: Synthèse spectrale de jeunes populations stellaires dans; l'ultraviolet lointain

NASA Astrophysics Data System (ADS)

Pellerin, Anne

2005-02-01

Le but de cette thèse était de développer et tester la technique de synthè;se spectrale évolutive aux longueurs d'onde de l'ultraviolet lointain. Jusquà récemment, cette technique n'était appliqué quà des données au-delà de 1200 Å. Le lancement du satellite FUSE en 1999 a permis d'explorer le domaine de l'ultraviolet lointain (900-1200 Å) avec une grande résolution spectrale. J'ai donc utilisé les spectres du satellite FUSE de 228 étoiles chaudes de type O et B, de 24 galaxies à sursauts de formation d'étoiles et de quatre galaxies Seyfert. Dans un premier temps, j'ai caractérisé le comportement des profils de raies stellaires en fonction du type spectral, de la classe de luminosité et de la métallicité des étoiles. Les raies O vi >>1031.9, 1037.6, S iv >>1062.7, 1073.0, 1073.5, P v>>1118.0, 1128.0 et C iii >1175.6 ont été identifiées comme étant des indicateurs stellaires potentiellement intéressants pour la synthèse spectrale. Le domaine de longueur d'onde inférieur à 1000 Å couvert par FUSEmontre aussi des signatures stellaires mais qui sont peu intéressantes pour la synthèse en raison de la contamination interstellaire. J'ai ensuite crééé; une bibliothèque de spectres FUSE qui a été intégrée au code de synthèse LavalSBafin de produire des spectres de synthèse dans l'ultraviolet lointain pour diverses populations stellaires théoriques. Il s'est avéré que les raies de P vet de C iii sont d'excellents indicateurs d'âge, de métallicité et de fonction de masse initiale de la population stellaire, tandis que les raies de O vi et de S ivne sont pas aussi efficaces. La comparaison des spectres FUSEde galaxies avec les spectres synthétiques a révèlé des âges entre 2.5 et 18 millions d'années pour un large éventail de métallicités. On trouve aussi une forte dominance du mode instantané de formation stellaire. Ce travail a aussi permis d'estimer quantitativement l'extinction interne et les masses

Pancreatic tissue assessment using fluorescence and reflectance spectroscopy

NASA Astrophysics Data System (ADS)

Chandra, Malavika; Heidt, David; Simeone, Diane; McKenna, Barbara; Scheiman, James; Mycek, Mary-Ann

2007-07-01

The ability of multi-modal optical spectroscopy to detect signals from pancreatic tissue was demonstrated by studying human pancreatic cancer xenografts in mice and freshly excised human pancreatic tumor tissue. Measured optical spectra and fluorescence decays were correlated with tissue morphological and biochemical properties. The measured spectral features and decay times correlated well with expected pathological differences in normal, pancreatitis and adenocarcinoma tissue states. The observed differences between the fluorescence and reflectance properties of normal, pancreatitis and adenocarcinoma tissue indicate a possible application of multi-modal optical spectroscopy to differentiating between the three tissue classifications.

Automated road network extraction from high spatial resolution multi-spectral imagery

NASA Astrophysics Data System (ADS)

Zhang, Qiaoping

For the last three decades, the Geomatics Engineering and Computer Science communities have considered automated road network extraction from remotely-sensed imagery to be a challenging and important research topic. The main objective of this research is to investigate the theory and methodology of automated feature extraction for image-based road database creation, refinement or updating, and to develop a series of algorithms for road network extraction from high resolution multi-spectral imagery. The proposed framework for road network extraction from multi-spectral imagery begins with an image segmentation using the k-means algorithm. This step mainly concerns the exploitation of the spectral information for feature extraction. The road cluster is automatically identified using a fuzzy classifier based on a set of predefined road surface membership functions. These membership functions are established based on the general spectral signature of road pavement materials and the corresponding normalized digital numbers on each multi-spectral band. Shape descriptors of the Angular Texture Signature are defined and used to reduce the misclassifications between roads and other spectrally similar objects (e.g., crop fields, parking lots, and buildings). An iterative and localized Radon transform is developed for the extraction of road centerlines from the classified images. The purpose of the transform is to accurately and completely detect the road centerlines. It is able to find short, long, and even curvilinear lines. The input image is partitioned into a set of subset images called road component images. An iterative Radon transform is locally applied to each road component image. At each iteration, road centerline segments are detected based on an accurate estimation of the line parameters and line widths. Three localization approaches are implemented and compared using qualitative and quantitative methods. Finally, the road centerline segments are grouped into a

A new multi-spectral feature level image fusion method for human interpretation

NASA Astrophysics Data System (ADS)

Leviner, Marom; Maltz, Masha

2009-03-01

Various different methods to perform multi-spectral image fusion have been suggested, mostly on the pixel level. However, the jury is still out on the benefits of a fused image compared to its source images. We present here a new multi-spectral image fusion method, multi-spectral segmentation fusion (MSSF), which uses a feature level processing paradigm. To test our method, we compared human observer performance in a three-task experiment using MSSF against two established methods: averaging and principle components analysis (PCA), and against its two source bands, visible and infrared. The three tasks that we studied were: (1) simple target detection, (2) spatial orientation, and (3) camouflaged target detection. MSSF proved superior to the other fusion methods in all three tests; MSSF also outperformed the source images in the spatial orientation and camouflaged target detection tasks. Based on these findings, current speculation about the circumstances in which multi-spectral image fusion in general and specific fusion methods in particular would be superior to using the original image sources can be further addressed.

Molecular hydrogen fluorescence and accretion in far-ultraviolet spectra of classical T Tauri stars

NASA Astrophysics Data System (ADS)

Herczeg, Gregory J.

2005-11-01

Far-ultraviolet spectra of classical T Tauri stars reveal accretion, outflows, and H 2 fluorescence. The E140M echelle spectrograph on HST /STIS and the FUSE satellite offer high spectral resolution and broad wavelength coverage, and enables our unique and detailed analysis of the H 2 lines. A strong and broad Lya emission line excites warm H 2 into many levels of the B and C electronic states, from which we can detect as many as 200 H 2 emission lines. These H2 lines are narrow and often asymmetric, with excess blueshifted emission that can extend to 100 km s -1 from some sources. The fluorescent H 2 emission probes diverse environments around CTTSs. High spectral and spatial resolution are essential for identifying the location and studying the kinematics of the gas, which constrain the origin of the H 2 emission. Several other spectral characteristics, including absorption of H2 emission by the wind and H 2 absorption lines, also provide valuable diagnostics of the origin of this emission. The H 2 emission is most likely produced at the surface of a circumstellar disk in some sources, but is produced by outflows from other sources. DF Tau appears to show H 2 emission from both a disk and an outflow. The excitation of H 2 can be determined from relative line strengths by measuring self-absorption in lines with low-energy lower levels, or by reconstructing the Lya profile incident upon the warm H 2 using the total flux from a single upper level and the opacity in the pumping transition. Based on those diagnostics and the rich H 2 spectrum of TW Hya, the H 2 at the warm disk surface has a column density of log N (H 2 ) = [Special characters omitted.] , a temperature T = [Special characters omitted.] K, and a filling factor of H 2 , as seen by the source of Lya emission, of 0.25 +/- 0.08 (all 2s error bars). The total FUV luminosity from CTTSs ranges from 2 x 10 -3 to 3 x 10 -2 [Special characters omitted.] , much of which is in the Lya line. With the exception of

Analysis of Spectral Features of Seawaterbiooptical Components Fluorescence from the Excitation-emission Matrix

NASA Astrophysics Data System (ADS)

Salyuk, P. A.; Nagorny, I. G.

The paper presents the method for processing of excitation-emission matrix of sea water and the allocation of the spectral characteristics of different types of colored dissolved organic matter (CDOM) and phytoplankton cells in seawater. The method consists of identification of regularly observed fluorescence peaks of CDOM in marine waters of different type and definition of the spectral ranges, where the predominant influence of these peaks are observed.

Complementary shifts in photoreceptor spectral tuning unlock the full adaptive potential of ultraviolet vision in birds.

PubMed

Toomey, Matthew B; Lind, Olle; Frederiksen, Rikard; Curley, Robert W; Riedl, Ken M; Wilby, David; Schwartz, Steven J; Witt, Christopher C; Harrison, Earl H; Roberts, Nicholas W; Vorobyev, Misha; McGraw, Kevin J; Cornwall, M Carter; Kelber, Almut; Corbo, Joseph C

2016-07-12

Color vision in birds is mediated by four types of cone photoreceptors whose maximal sensitivities (λmax) are evenly spaced across the light spectrum. In the course of avian evolution, the λmax of the most shortwave-sensitive cone, SWS1, has switched between violet (λmax > 400 nm) and ultraviolet (λmax < 380 nm) multiple times. This shift of the SWS1 opsin is accompanied by a corresponding short-wavelength shift in the spectrally adjacent SWS2 cone. Here, we show that SWS2 cone spectral tuning is mediated by modulating the ratio of two apocarotenoids, galloxanthin and 11’,12’-dihydrogalloxanthin, which act as intracellular spectral filters in this cell type. We propose an enzymatic pathway that mediates the differential production of these apocarotenoids in the avian retina, and we use color vision modeling to demonstrate how correlated evolution of spectral tuning is necessary to achieve even sampling of the light spectrum and thereby maintain near-optimal color discrimination.

The role of ultraviolet-A reflectance and ultraviolet-A induced fluorescence in the appearance of budgerigar plumage: insights from spectrofluorometry and reflectance spectrophotometry.

PubMed Central

Pearn, Sophie M; Bennett, Andrew T D; Cuthill, Innes C

2003-01-01

Fluorescence has so far been found in 52 parrot species when illuminated with ultraviolet-A (UVA) 'black' lamps, and two attempts have been made to determine whether such fluorescence plays any role in sexual signalling. However, the contribution of the reflectance versus fluorescence to the total radiance from feathers, even in the most studied species to date (budgerigars), is unclear. Nor has the plumage of this study species been systematically assessed to determine the distribution of fluorescent patches. We therefore used spectrofluorometry to determine which areas of budgerigars fluoresce and the excitation and emission spectra involved; this is the first time that such a technique has been applied to avian plumage. We found that both the yellow crown and (normally hidden) white downy chest feathers exhibit strong UVA-induced fluorescence, with peak emissions at 527 nm and 436 nm, respectively. Conversely, the bright-green chest and dark-blue tail feathers do not fluoresce. When comparing reflectance spectra (400-700 nm) from the yellow crown using illuminants with a proportion of UVA comparable to daylight, and illuminants with all UVA removed, no measurable difference resulting from fluorescence was found. This suggests that under normal daylight the contribution of fluorescence to radiance is probably trivial. Furthermore, these spectra revealed that males had fluorescent crowns with substantially higher reflectance than those of females, in both the UV waveband and at longer wavelengths. Reflectance spectrophotometry was also performed on a number of live wild-type male budgerigars to investigate the chromatic contrast between the different plumage areas. This showed that many plumage regions are highly UV-reflective. Overall our results suggest that rapid surveys using UVA black lamps may overestimate the contribution of fluorescence to plumage coloration, and that any signalling role of fluorescence emissions, at least from the yellow crown of

The Loopy Ultraviolet Line Profiles of RU Lupi: Accretion, Outflows, and Fluorescence

NASA Astrophysics Data System (ADS)

Herczeg, Gregory J.; Walter, Frederick M.; Linsky, Jeffrey L.; Gahm, Gösta F.; Ardila, David R.; Brown, Alexander; Johns-Krull, Christopher M.; Simon, Michal; Valenti, Jeff A.

2005-06-01

We present far-ultraviolet (FUV) spectra of the classical T Tauri star RU Lup covering the 912-1710 Å spectral range, as observed by the Hubble Space Telescope STIS and the Far Ultraviolet Spectroscopic Explorer satellite. We use these spectra, which are rich in emission and absorption lines, to probe both the accreting and outflowing gas. Absorption in the Lyα profile constrains the extinction to AV~0.07 mag, which we confirm with other diagnostics. We estimate a mass accretion rate of (5+/-2)×10-8 Msolar yr-1 using the optical-NUV accretion continuum. The accreting gas is also detected in bright, broad lines of C IV, Si IV, and N V, which all show complex structures across the line profile. Many other emission lines, including those of H2 and Fe II, are pumped by Lyα. RU Lup's spectrum varies significantly in the FUV; our STIS observations occurred when RU Lup was brighter than several other observations in the FUV, possibly because of a high mass accretion rate.

Visual perception enhancement for detection of cancerous oral tissue by multi-spectral imaging

NASA Astrophysics Data System (ADS)

Wang, Hsiang-Chen; Tsai, Meng-Tsan; Chiang, Chun-Ping

2013-05-01

Color reproduction systems based on the multi-spectral imaging technique (MSI) for both directly estimating reflection spectra and direct visualization of oral tissues using various light sources are proposed. Images from three oral cancer patients were taken as the experimental samples, and spectral differences between pre-cancerous and normal oral mucosal tissues were calculated at three time points during 5-aminolevulinic acid photodynamic therapy (ALA-PDT) to analyze whether they were consistent with disease processes. To check the successful treatment of oral cancer with ALA-PDT, oral cavity images by swept source optical coherence tomography (SS-OCT) are demonstrated. This system can also reproduce images under different light sources. For pre-cancerous detection, the oral images after the second ALA-PDT are assigned as the target samples. By using RGB LEDs with various correlated color temperatures (CCTs) for color difference comparison, the light source with a CCT of about 4500 K was found to have the best ability to enhance the color difference between pre-cancerous and normal oral mucosal tissues in the oral cavity. Compared with the fluorescent lighting commonly used today, the color difference can be improved by 39.2% from 16.5270 to 23.0023. Hence, this light source and spectral analysis increase the efficiency of the medical diagnosis of oral cancer and aid patients in receiving early treatment.

Spectral Fluorescence Properties of an Anionic Oxacarbocyanine Dye in Complexes with Human Serum Albumin

NASA Astrophysics Data System (ADS)

Pronkin, P. G.; Tatikolov, A. S.

2015-07-01

The spectral fluorescence properties of the anionic oxacarbocyanine dye 3,3'-di-(γ-sulfopropyl)-5,5'-diphenyl-9-ethyloxacarbocyanine betaine (OCC) were studied in solutions and in complexes with human serum albumin (HSA). Interaction with HSA leads to a significant increase in the fluorescence of the dye. We studied quenching of the fluorescence of OCC in a complex with HSA by ibuprofen and warfarin. Data on quenching of fluorescence by ibuprofen indicate binding of the dye to binding site II of subdomain IIIA in the HSA molecule. Synchronous fluorescence spectra of human serum albumin in the presence of OCC showed that complexation with OCC does not lead to appreciable rearrangement of the protein molecule at the binding site.

A conjunct near-surface spectroscopy system for fix-angle and multi-angle continuous measurements of canopy reflectance and sun-induced chlorophyll fluorescence

NASA Astrophysics Data System (ADS)

Zhang, Qian; Fan, Yifeng; Zhang, Yongguang; Chou, Shuren; Ju, Weimin; Chen, Jing M.

2016-09-01

An automated spectroscopy system, which is divided into fix-angle and multi-angle subsystems, for collecting simultaneous, continuous and long-term measurements of canopy hyper-spectra in a crop ecosystem is developed. The fix-angle subsystem equips two spectrometers: one is HR2000+ (OceanOptics) covering the spectral range 200-1100 nm with 1.0 nm spectral resolution, and another one is QE65PRO (OceanOptics) providing 0.1 nm spectral resolution within the 730-780 nm spectral range. Both spectrometers connect a cosine-corrected fiber-optic fixed up-looking to collect the down-welling irradiance and a bare fiber-optic to measure the up-welling radiance from the vegetation. An inline fiber-optic shutter FOS-2x2-TTL (OceanOptics) is used to switch between input fibers to collect the signal from either the canopy or sky at one time. QE65PRO is used to permit estimation of vegetation Sun-Induced Fluorescence (SIF) in the O2-A band. The data collection scheme includes optimization of spectrometer integration time to maximize the signal to noise ratio and measurement of instrument dark currency. The multi-angle subsystem, which can help understanding bidirectional reflectance effects, alternatively use HR4000 (OceanOptics) providing 0.1 nm spectral resolution within the 680-800 nm spectral range to measure multi-angle SIF. This subsystem additionally includes a spectrometer Unispec-DC (PPSystems) featuring both up-welling and down-welling channels with 3 nm spectral resolution covering the 300-1100 nm spectral range. Two down-looking fiber-optics are mounted on a rotating device PTU-D46 (FLIR Systems), which can rotate horizontally and vertically at 10° angular step widths. Observations can be used to calculate canopy reflectance, vegetation indices and SIF for monitoring plant physiological processes.

Spectrally resolving and scattering-compensated x-ray luminescence/fluorescence computed tomography

PubMed Central

Cong, Wenxiang; Shen, Haiou; Wang, Ge

2011-01-01

The nanophosphors, or other similar materials, emit near-infrared (NIR) light upon x-ray excitation. They were designed as optical probes for in vivo visualization and analysis of molecular and cellular targets, pathways, and responses. Based on the previous work on x-ray fluorescence computed tomography (XFCT) and x-ray luminescence computed tomography (XLCT), here we propose a spectrally-resolving and scattering-compensated x-ray luminescence/fluorescence computed tomography (SXLCT or SXFCT) approach to quantify a spatial distribution of nanophosphors (other similar materials or chemical elements) within a biological object. In this paper, the x-ray scattering is taken into account in the reconstruction algorithm. The NIR scattering is described in the diffusion approximation model. Then, x-ray excitations are applied with different spectra, and NIR signals are measured in a spectrally resolving fashion. Finally, a linear relationship is established between the nanophosphor distribution and measured NIR data using the finite element method and inverted using the compressive sensing technique. The numerical simulation results demonstrate the feasibility and merits of the proposed approach. PMID:21721815

Nontemplated Approach to Tuning the Spectral Propertiesof Cyanine-Based Fluorescent NanoGUMBOS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Das, Susmita; Bwambok, David; El-Zahab, Bilal

2010-01-01

Template-free controlled aggregation and spectral properties in fluorescent organic nanoparticles (FONs) is highly desirable for various applications.Herein, we report a nontemplated method for controlling the aggregation in near-infrared (NIR) cyanine-based nanoparticles derived from a group of uniformmaterials based on organic salts (GUMBOS). Cationic heptamethine cyanine dye 1,10,3,3,30,30-hexamethylindotricarbocyanine (HMT) was coupled with five different anions, viz., [NTf2 -], [BETI-], [TFPB-], [AOT-], and [TFP4B-], by an ion-exchange method to obtain the respective GUMBOS. The nanoGUMBOS obtained via a reprecipitation method were primarily amorphous and spherical (30-100 nm) as suggested by selected area electron diffraction (SAED) and transmission electron microscopy (TEM). The formationmore » of tunable self-assemblies within the nanoGUMBOS was characterized using absorption and fluorescence spectroscopy in conjunction with molecular dynamics simulations. Counterion-controlled spectral properties observed in the nanoGUMBOS were attributed to variations in J/H ratios with different anions. Association with the [AOT-] anion afforded predominant J aggregation enabling the highest fluorescence intensity, whereas [TFP4B-] disabled the fluorescence due to predominantHaggregation in the nanoparticles. Analyses of the stacking angle of the cations based on molecular dynamic simulation results in [HMT][NTf2], [HMT][BETI], and [HMT][AOT] dispersed in water and a visual analysis of the representative simulation snapshots also imply that the type of aggregation was controlled through the counterion associated with the dye cation.« less

Fluorescence quantum yield of carbon dioxide for quantitative UV laser-induced fluorescence in high-pressure flames

NASA Astrophysics Data System (ADS)

Lee, T.; Bessler, W. G.; Yoo, J.; Schulz, C.; Jeffries, J. B.; Hanson, R. K.

2008-11-01

The fluorescence quantum yield for ultraviolet laser-induced fluorescence of CO2 is determined for selected excitation wavelengths in the range 215-250 nm. Wavelength-resolved laser-induced fluorescence (LIF) spectra of CO2, NO, and O2 are measured in the burned gases of a laminar CH4/air flame ( φ=0.9 and 1.1) at 20 bar with additional NO seeded into the flow. The fluorescence spectra are fit to determine the relative contribution of the three species to infer an estimate of fluorescence quantum yield for CO2 that ranges from 2-8×10-6 depending on temperature and excitation wavelength with an estimated uncertainty of ±0.5×10-6. The CO2 fluorescence signal increases linearly with gas pressure for flames with constant CO2 mole fraction for the 10 to 60 bar range, indicating that collisional quenching is not an important contributor to the CO2 fluorescence quantum yield. Spectral simulation calculations are used to choose two wavelengths for excitation of CO2, 239.34 and 242.14 nm, which minimize interference from LIF of NO and O2. Quantitative LIF images of CO2 are demonstrated using these two excitation wavelengths and the measured fluorescence quantum yield.

Discrimination of corn from monocotyledonous weeds with ultraviolet (UV) induced fluorescence.

PubMed

Panneton, Bernard; Guillaume, Serge; Samson, Guy; Roger, Jean-Michel

2011-01-01

In production agriculture, savings in herbicides can be achieved if weeds can be discriminated from crop, allowing the targeting of weed control to weed-infested areas only. Previous studies demonstrated the potential of ultraviolet (UV) induced fluorescence to discriminate corn from weeds and recently, robust models have been obtained for the discrimination between monocots (including corn) and dicots. Here, we developed a new approach to achieve robust discrimination of monocot weeds from corn. To this end, four corn hybrids (Elite 60T05, Monsanto DKC 26-78, Pioneer 39Y85 (RR), and Syngenta N2555 (Bt, LL)) and four monocot weeds (Digitaria ischaemum (Schreb.) I, Echinochloa crus-galli (L.) Beauv., Panicum capillare (L.), and Setaria glauca (L.) Beauv.) were grown either in a greenhouse or in a growth cabinet and UV (327 nm) induced fluorescence spectra (400 to 755 nm) were measured under controlled or uncontrolled ambient light intensity and temperature. This resulted in three contrasting data sets suitable for testing the robustness of discrimination models. In the blue-green region (400 to 550 nm), the shape of the spectra did not contain any useful information for discrimination. Therefore, the integral of the blue-green region (415 to 455 nm) was used as a normalizing factor for the red fluorescence intensity (670 to 755 nm). The shape of the normalized red fluorescence spectra did not contribute to the discrimination and in the end, only the integral of the normalized red fluorescence intensity was left as a single discriminant variable. Applying a threshold on this variable minimizing the classification error resulted in calibration errors ranging from 14.2% to 15.8%, but this threshold varied largely between data sets. Therefore, to achieve robustness, a model calibration scheme was developed based on the collection of a calibration data set from 75 corn plants. From this set, a new threshold can be estimated as the 85% quantile on the cumulative frequency

Multi-pass encoding of hyperspectral imagery with spectral quality control

NASA Astrophysics Data System (ADS)

Wasson, Steven; Walker, William

2015-05-01

Multi-pass encoding is a technique employed in the field of video compression that maximizes the quality of an encoded video sequence within the constraints of a specified bit rate. This paper presents research where multi-pass encoding is extended to the field of hyperspectral image compression. Unlike video, which is primarily intended to be viewed by a human observer, hyperspectral imagery is processed by computational algorithms that generally attempt to classify the pixel spectra within the imagery. As such, these algorithms are more sensitive to distortion in the spectral dimension of the image than they are to perceptual distortion in the spatial dimension. The compression algorithm developed for this research, which uses the Karhunen-Loeve transform for spectral decorrelation followed by a modified H.264/Advanced Video Coding (AVC) encoder, maintains a user-specified spectral quality level while maximizing the compression ratio throughout the encoding process. The compression performance may be considered near-lossless in certain scenarios. For qualitative purposes, this paper presents the performance of the compression algorithm for several Airborne Visible/Infrared Imaging Spectrometer (AVIRIS) and Hyperion datasets using spectral angle as the spectral quality assessment function. Specifically, the compression performance is illustrated in the form of rate-distortion curves that plot spectral angle versus bits per pixel per band (bpppb).

Open-source do-it-yourself multi-color fluorescence smartphone microscopy

PubMed Central

Sung, Yulung; Campa, Fernando; Shih, Wei-Chuan

2017-01-01

Fluorescence microscopy is an important technique for cellular and microbiological investigations. Translating this technique onto a smartphone can enable particularly powerful applications such as on-site analysis, on-demand monitoring, and point-of-care diagnostics. Current fluorescence smartphone microscope setups require precise illumination and imaging alignment which altogether limit its broad adoption. We report a multi-color fluorescence smartphone microscope with a single contact lens-like add-on lens and slide-launched total-internal-reflection guided illumination for three common tasks in investigative fluorescence microscopy: autofluorescence, fluorescent stains, and immunofluorescence. The open-source, simple and cost-effective design has the potential for do-it-yourself fluorescence smartphone microscopy. PMID:29188104

Investigating the 3.3 micron infrared fluorescence from naphthalene following ultraviolet excitation

NASA Technical Reports Server (NTRS)

Williams, Richard M.; Leone, Stephen R.

1994-01-01

Polycyclic aromatic hydrocarbon (PAH) type molecules are proposed as the carriers of the unidentified infrared (UIR) bands. Detailed studies of the 3.3 micrometer infrared emission features from naphthalene, the simplest PAH, following ultraviolet laser excitation are used in the interpretation of the 3.29 micrometer (3040 cm(sup -1)) UIR band. A time-resolved Fourier transform spectrometer is used to record the infrared emission spectrum of gas-phase naphthalene subsequent to ultraviolet excitation facilitated by an excimer laser operated at either 193 nm or 248 nm. The emission spectra differ significantly from the absorption spectrum in the same spectral region. Following 193 nm excitation the maximum in the emission profile is red-shifted 45 cm(sup -1) relative to the absorption maximum; a 25 cm(sup -1) red-shift is observed after 248 nm excitation. The red-shifting of the emission spectrum is reduced as collisional and radiative relaxation removes energy from the highly vibrationally excited molecules. Coupling between the various vibrational modes is thought to account for the differences between absorption and emission spectra. Strong visible emission is also observed following ultraviolet excitation. Visible emission may play an important role in the rate of radiative relaxation, which according to the interstellar PAH hypothesis occurs only by the slow emission of infrared photons. Studying the visible emission properties of PAH type molecules may be useful in the interpretation of the DIB's observed in absorption.

A Detailed Far-ultraviolet Spectral Atlas of O-type Stars

NASA Astrophysics Data System (ADS)

Smith, Myron A.

2012-10-01

In this paper, we present a spectral atlas covering the wavelength interval 930-1188 Å for O2-O9.5 stars using Far-Ultraviolet Spectroscopic Explorer archival data. The stars selected for the atlas were drawn from three populations: Galactic main-sequence (classes III-V) stars, supergiants, and main-sequence stars in the Magellanic Clouds, which have low metallicities. For several of these stars, we have prepared FITS files comprised of pairs of merged spectra for user access via the Multimission Archive at Space Telescope (MAST). We chose spectra from the first population with spectral types O4, O5, O6, O7, O8, and O9.5 and used them to compile tables and figures with identifications of all possible atmospheric and interstellar medium lines in the region 949-1188 Å. Our identified line totals for these six representative spectra are 821 (500), 992 (663), 1077 (749), 1178 (847), 1359 (1001), and 1798 (1392) lines, respectively, where the numbers in parentheses are the totals of lines formed in the atmospheres, according to spectral synthesis models. The total number of unique atmospheric identifications for the six main-sequence O-star template spectra is 1792, whereas the number of atmospheric lines in common to these spectra is 300. The number of identified lines decreases toward earlier types (increasing effective temperature), while the percentages of "missed" features (unknown lines not predicted from our spectral syntheses) drop from a high of 8% at type B0.2, from our recently published B-star far-UV atlas, to 1%-3% for type O spectra. The percentages of overpredicted lines are similar, despite their being much higher for B-star spectra. We discuss the statistics of line populations among the various elemental ionization states. Also, as an aid to users we list those isolated lines that can be used to determine stellar temperatures and the presence of possible chemical anomalies. Finally, we have prepared FITS files that give pairs of merged spectra for

Test of multi-object exoplanet search spectral interferometer

NASA Astrophysics Data System (ADS)

Zhang, Kai; Wang, Liang; Jiang, Haijiao; Zhu, Yongtian; Hou, Yonghui; Dai, Songxin; Tang, Jin; Tang, Zhen; Zeng, Yizhong; Chen, Yi; Wang, Lei; Hu, Zhongwen

2014-07-01

Exoplanet detection, a highlight in the current astronomy, will be part of puzzle in astronomical and astrophysical future, which contains dark energy, dark matter, early universe, black hole, galactic evolution and so on. At present, most of the detected Exoplanets are confirmed through methods of radial velocity and transit. Guo shoujing Telescope well known as LAMOST is an advanced multi-object spectral survey telescope equipped with 4000 fibers and 16 low resolution fiber spectrographs. To explore its potential in different astronomical activities, a new radial velocity method named Externally Dispersed Interferometry (EDI) is applied to serve Exoplanet detection through combining a fixed-delay interferometer with the existing spectrograph in medium spectral resolution mode (R=5,000-10,000). This new technology has an impressive feature to enhance radial velocity measuring accuracy of the existing spectrograph through installing a fixed-delay interferometer in front of spectrograph. This way produces an interference spectrum with higher sensitivity to Doppler Effect by interference phase and fixed delay. This relative system named Multi-object Exoplanet Search Spectral Interferometer (MESSI) is composed of a few parts, including a pair of multi-fiber coupling sockets, a remote control iodine subsystem, a multi-object fixed delay interferometer and the existing spectrograph. It covers from 500 to 550 nm and simultaneously observes up to 21 stars. Even if it's an experimental instrument at present, it's still well demonstrated in paper that how MESSI does explore an effective way to build its own system under the existing condition of LAMOST and get its expected performance for multi-object Exoplanet detection, especially instrument stability and its special data reduction. As a result of test at lab, inside temperature of its instrumental chamber is stable in a range of +/-0.5degree Celsius within 12 hours, and the direct instrumental stability without further

Spectral Diversity and Regulation of Coral Fluorescence in a Mesophotic Reef Habitat in the Red Sea.

PubMed

Eyal, Gal; Wiedenmann, Jörg; Grinblat, Mila; D'Angelo, Cecilia; Kramarsky-Winter, Esti; Treibitz, Tali; Ben-Zvi, Or; Shaked, Yonathan; Smith, Tyler B; Harii, Saki; Denis, Vianney; Noyes, Tim; Tamir, Raz; Loya, Yossi

2015-01-01

The phenomenon of coral fluorescence in mesophotic reefs, although well described for shallow waters, remains largely unstudied. We found that representatives of many scleractinian species are brightly fluorescent at depths of 50-60 m at the Interuniversity Institute for Marine Sciences (IUI) reef in Eilat, Israel. Some of these fluorescent species have distribution maxima at mesophotic depths (40-100 m). Several individuals from these depths displayed yellow or orange-red fluorescence, the latter being essentially absent in corals from the shallowest parts of this reef. We demonstrate experimentally that in some cases the production of fluorescent pigments is independent of the exposure to light; while in others, the fluorescence signature is altered or lost when the animals are kept in darkness. Furthermore, we show that green-to-red photoconversion of fluorescent pigments mediated by short-wavelength light can occur also at depths where ultraviolet wavelengths are absent from the underwater light field. Intraspecific colour polymorphisms regarding the colour of the tissue fluorescence, common among shallow water corals, were also observed for mesophotic species. Our results suggest that fluorescent pigments in mesophotic reefs fulfil a distinct biological function and offer promising application potential for coral-reef monitoring and biomedical imaging.

Molecular fluorescence as a monitor of minor stratospheric constituents

NASA Technical Reports Server (NTRS)

Schofield, K.

1975-01-01

The potential of molecular fluorescence was assessed as a stratospheric monitor of the concentrations of various minor species. Seventeen molecules were considered and all spectral regions from the vacuum ultraviolet through to the infrared are included. The exercise has produced few surprises; however, further confirmation has emerged as to the feasibility of this sensitive technique for monitoring stratospheric OH, NO2, and SO2, sufficient to warrant its development as analytical flight hardware for these species. All the other molecules have been eliminated with the exception of C10 which appears to have promise but requires additional information before its detection limit can be calculated; its fluorescence spectrum is as yet uninvestigated. A handbook for molecular fluorescence is presented, and a compilation of all reported studies for simple molecules using line source excitation is included.

Complementary shifts in photoreceptor spectral tuning unlock the full adaptive potential of ultraviolet vision in birds

PubMed Central

Toomey, Matthew B; Lind, Olle; Frederiksen, Rikard; Curley, Robert W; Riedl, Ken M; Wilby, David; Schwartz, Steven J; Witt, Christopher C; Harrison, Earl H; Roberts, Nicholas W; Vorobyev, Misha; McGraw, Kevin J; Cornwall, M Carter; Kelber, Almut; Corbo, Joseph C

2016-01-01

Color vision in birds is mediated by four types of cone photoreceptors whose maximal sensitivities (λmax) are evenly spaced across the light spectrum. In the course of avian evolution, the λmax of the most shortwave-sensitive cone, SWS1, has switched between violet (λmax > 400 nm) and ultraviolet (λmax < 380 nm) multiple times. This shift of the SWS1 opsin is accompanied by a corresponding short-wavelength shift in the spectrally adjacent SWS2 cone. Here, we show that SWS2 cone spectral tuning is mediated by modulating the ratio of two apocarotenoids, galloxanthin and 11’,12’-dihydrogalloxanthin, which act as intracellular spectral filters in this cell type. We propose an enzymatic pathway that mediates the differential production of these apocarotenoids in the avian retina, and we use color vision modeling to demonstrate how correlated evolution of spectral tuning is necessary to achieve even sampling of the light spectrum and thereby maintain near-optimal color discrimination. DOI: http://dx.doi.org/10.7554/eLife.15675.001 PMID:27402384

Intrinsic Fluorescence as a Spectral Probe for Protein Denaturation Studies in the Presence of Honey

NASA Astrophysics Data System (ADS)

Wong, Y. H.; Kadir, H. A.; Tayyab, S.

2015-11-01

Honey was found to quench the intrinsic fluorescence of bovine serum albumin (BSA) in a concentration dependent manner, showing complete quenching in the presence of 5% (w/v) honey. Increasing the protein concentration up to 5.0 μM did not lead to the recovery of the protein fluorescence. Urea denaturation of BSA, which otherwise shows a two-step, three-state transition, using intrinsic fluorescence of the protein as the probe failed to produce any result in the presence of 5% (w/v) honey. Thus, intrinsic fluorescence cannot be used as a spectral probe for protein denaturation studies in the presence of honey.

Multi-objective based spectral unmixing for hyperspectral images

NASA Astrophysics Data System (ADS)

Xu, Xia; Shi, Zhenwei

2017-02-01

Sparse hyperspectral unmixing assumes that each observed pixel can be expressed by a linear combination of several pure spectra in a priori library. Sparse unmixing is challenging, since it is usually transformed to a NP-hard l0 norm based optimization problem. Existing methods usually utilize a relaxation to the original l0 norm. However, the relaxation may bring in sensitive weighted parameters and additional calculation error. In this paper, we propose a novel multi-objective based algorithm to solve the sparse unmixing problem without any relaxation. We transform sparse unmixing to a multi-objective optimization problem, which contains two correlative objectives: minimizing the reconstruction error and controlling the endmember sparsity. To improve the efficiency of multi-objective optimization, a population-based randomly flipping strategy is designed. Moreover, we theoretically prove that the proposed method is able to recover a guaranteed approximate solution from the spectral library within limited iterations. The proposed method can directly deal with l0 norm via binary coding for the spectral signatures in the library. Experiments on both synthetic and real hyperspectral datasets demonstrate the effectiveness of the proposed method.

Multicolor Super-Resolution Fluorescence Imaging via Multi-Parameter Fluorophore Detection

PubMed Central

Bates, Mark; Dempsey, Graham T; Chen, Kok Hao; Zhuang, Xiaowei

2012-01-01

Understanding the complexity of the cellular environment will benefit from the ability to unambiguously resolve multiple cellular components, simultaneously and with nanometer-scale spatial resolution. Multicolor super-resolution fluorescence microscopy techniques have been developed to achieve this goal, yet challenges remain in terms of the number of targets that can be simultaneously imaged and the crosstalk between color channels. Herein, we demonstrate multicolor stochastic optical reconstruction microscopy (STORM) based on a multi-parameter detection strategy, which uses both the fluorescence activation wavelength and the emission color to discriminate between photo-activatable fluorescent probes. First, we obtained two-color super-resolution images using the near-infrared cyanine dye Alexa 750 in conjunction with a red cyanine dye Alexa 647, and quantified color crosstalk levels and image registration accuracy. Combinatorial pairing of these two switchable dyes with fluorophores which enhance photo-activation enabled multi-parameter detection of six different probes. Using this approach, we obtained six-color super-resolution fluorescence images of a model sample. The combination of multiple fluorescence detection parameters for improved fluorophore discrimination promises to substantially enhance our ability to visualize multiple cellular targets with sub-diffraction-limit resolution. PMID:22213647

Retrieving Biome Types from Multi-angle Spectral Data

NASA Astrophysics Data System (ADS)

Schull, M. A.; Xu, L.; Latorre, P.; Samanta, A.; Myneni, R. B.; Knyazikhin, Y.

2009-12-01

Many studies have been conducted to demonstrate the ability of multi-angle spectral data to discriminate plant dominant species. Most have employed the use of empirically based techniques, which are site specific, requires some initial training based on characteristics of known leaf and/or canopy spectra and therefore may not be extendable to operational use or adapted to changing/unknown land cover. An ancillary objective of the MISR LAI/FPAR algorithm is classification of global vegetation into biome types. The algorithm is based on the 3D radiative transfer equation. Its performance suggests that is has valid LAI retrievals and correct biome identification in about 20% of the pixels. However with a probability of about 70%, uncertainties in LAI retrievals due to biome misclassification do not exceed uncertainties in the observations. In this poster we present an approach to improve reliability of the distribution of biomes and dominant species from multi angle spectral data. The radiative transfer theory of canopy spectral invariants underlies the approach, which facilitates parameterization of the canopy bidirectional reflectance factor in terms of the leaf spectrum and two spectrally invariant and structurally varying variables - recollision and directional escape probabilities. Theoretical and empirical analyses of ground and airborne data acquired by AVIRIS, AirMISR over two sites in New England and CHRIS/PROBA over BARAX site in Spain suggest that the canopy spectral invariants convey information about canopy structure at both the macro and micro scales. These properties allow for the natural separation of biome classes based on the location of points on the total escape probability vs the proportional escape ratio log-log plane.

Two-photon optical imaging, spectral and fluorescence lifetime analysis to discriminate urothelial carcinoma grades.

PubMed

Pradère, B; Poulon, F; Compérat, E; Lucas, I; Bazin, D; Doizi, S; Cussenot, O; Traxer, O; Abi Haidar, D

2018-05-28

In the framework of urologic oncology, mini-invasive procedures have increased in the last few decades particularly for urothelial carcinoma. One of the essential elements in the management of this disease is still the diagnosis, which strongly influences the choice of treatment. The histopathologic evaluation of the tumor grade is a keystone of diagnosis, and tumor characterization is not possible with just a macroscopic evaluation. Even today intraoperative evaluation remains difficult despite the emergence of new technologies which use exogenous fluorophore. This study assessed an optical multimodal technique based on endogenous fluorescence, combining qualitative and quantitative analysis, for the diagnostic of urothelial carcinoma. It was found that the combination of two photon fluorescence, second harmonic generation microscopy, spectral analysis and fluorescence lifetime imaging were all able to discriminate tumor from healthy tissue, and to determine the grade of tumors. Spectral analysis of fluorescence intensity and the redox ratio used as quantitative evaluations showed statistical differences between low grade and high grade tumors. These results showed that multimodal optical analysis is a promising technology for the development of an optical fiber setup designed for an intraoperative diagnosis of urothelial carcinoma in the area of endourology. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Time-resolved detection of aromatic compounds on planetary surfaces by ultraviolet laser induced fluorescence and Raman spectroscopy

NASA Astrophysics Data System (ADS)

Eshelman, E.; Daly, M. G.; Slater, G.; Cloutis, E.

2015-12-01

Raman spectroscopic instruments are highly capable in the search for organics on Mars due to the potential to perform rapid and nondestructive measurements on unprepared samples. Upcoming and future Raman instruments are likely to also incorporate laser-induced fluorescence (LIF) capabilities, which can be added for modest cost and complexity. We demonstrate that it is possible to obtain sub-ns fluorescence lifetime measurements of Mars-relevant organics and minerals if a fast time-gating capability is used with an intensified detector and a short ultraviolet laser pulse. This serves a primary purpose of discriminating mineral from short-lived (less than 10 ns) organic fluorescence, considered a potential biosignature. Additionally, lifetime measurements may assist in determining if more than one fluorescing species is present and provide information concerning the molecular structure as well as the local environment. Fast time-gating is also useful at longer visible or near-IR wavelengths, as this approach increases the sensitivity of the instrument to organic material by removing the majority of the fluorescence background from the Raman signal and reducing the effect of ambient light.

Fluorescence imaging spectrometer optical design

NASA Astrophysics Data System (ADS)

Taiti, A.; Coppo, P.; Battistelli, E.

2015-09-01

The optical design of the FLuORescence Imaging Spectrometer (FLORIS) studied for the Fluorescence Explorer (FLEX) mission is discussed. FLEX is a candidate for the ESA's 8th Earth Explorer opportunity mission. FLORIS is a pushbroom hyperspectral imager foreseen to be embarked on board of a medium size satellite, flying in tandem with Sentinel-3 in a Sun synchronous orbit at a height of about 815 km. FLORIS will observe the vegetation fluorescence and reflectance within a spectral range between 500 and 780 nm. Multi-frames acquisitions on matrix detectors during the satellite movement will allow the production of 2D Earth scene images in two different spectral channels, called HR and LR with spectral resolution of 0.3 and 2 nm respectively. A common fore optics is foreseen to enhance by design the spatial co-registration between the two spectral channels, which have the same ground spatial sampling (300 m) and swath (150 km). An overlapped spectral range between the two channels is also introduced to simplify the spectral coregistration. A compact opto-mechanical solution with all spherical and plane optical elements is proposed, and the most significant design rationales are described. The instrument optical architecture foresees a dual Babinet scrambler, a dioptric telescope and two grating spectrometers (HR and LR), each consisting of a modified Offner configuration. The developed design is robust, stable vs temperature, easy to align, showing very high optical quality along the whole field of view. The system gives also excellent correction for transverse chromatic aberration and distortions (keystone and smile).

[Nitrogen stress measurement of canola based on multi-spectral charged coupled device imaging sensor].

PubMed

Feng, Lei; Fang, Hui; Zhou, Wei-Jun; Huang, Min; He, Yong

2006-09-01

Site-specific variable nitrogen application is one of the major precision crop production management operations. Obtaining sufficient crop nitrogen stress information is essential for achieving effective site-specific nitrogen applications. The present paper describes the development of a multi-spectral nitrogen deficiency sensor, which uses three channels (green, red, near-infrared) of crop images to determine the nitrogen level of canola. This sensor assesses the nitrogen stress by means of estimated SPAD value of the canola based on canola canopy reflectance sensed using three channels (green, red, near-infrared) of the multi-spectral camera. The core of this investigation is the calibration methods between the multi-spectral references and the nitrogen levels in crops measured using a SPAD 502 chlorophyll meter. Based on the results obtained from this study, it can be concluded that a multi-spectral CCD camera can provide sufficient information to perform reasonable SPAD values estimation during field operations.

Ultraviolet spectral variations of symbiotic nova PU Vul during and after second eclipse

NASA Astrophysics Data System (ADS)

Sanad, M. R.

2016-12-01

I have analyzed spectral data of the symbiotic nova PU Vul observed with the International Ultraviolet Explorer (IUE) during the period 1993-1996. The study concentrated on the two sources of nebular emitting regions, the first is a nebula around the white dwarf partially eclipsed by a cool giant star and the second is a very extended nebular region not affected by the eclipse of the giant star. I concentrated on the N IV] 1486 Å and C IV 1550 Å emission lines produced in the first region and N III] 1750 Å and C III] 1909 Å emission lines produced in the second region very far from the giant star.

High-power ultra-broadband frequency comb from ultraviolet to infrared by high-power fiber amplifiers.

PubMed

Yang, Kangwen; Li, Wenxue; Yan, Ming; Shen, Xuling; Zhao, Jian; Zeng, Heping

2012-06-04

A high-power ultra-broadband frequency comb covering the spectral range from ultraviolet to infrared was generated directly by nonlinear frequency conversion of a multi-stage high-power fiber comb amplifier. The 1030-nm infrared spectral fraction of a broadband Ti:sapphire femtosecond frequency comb was power-scaled up to 100 W average power by using a large-mode-area fiber chirped-pulse amplifier. We obtained a frequency-doubled green comb at 515 nm and frequency-quadrupled ultraviolet pulses at 258 nm with the average power of 12.8 and 1.62 W under the input infrared power of 42.2 W, respectively. The carrier envelope phase stabilization was accomplished with an ultra-narrow line-width of 1.86 mHz and a quite low accumulated phase jitter of 0.41 rad, corresponding to a timing jitter of 143 as.

Spectral Phasor approach for fingerprinting of photo-activatable fluorescent proteins Dronpa, Kaede and KikGR

PubMed Central

Cutrale, Francesco; Salih, Anya; Gratton, Enrico

2013-01-01

The phasor global analysis algorithm is common for fluorescence lifetime applications, but has only been recently proposed for spectral analysis. Here the phasor representation and fingerprinting is exploited in its second harmonic to determine the number and spectra of photo-activated states as well as their conversion dynamics. We follow the sequence of photo-activation of proteins over time by rapidly collecting multiple spectral images. The phasor representation of the cumulative images provides easy identification of the spectral signatures of each photo-activatable protein. PMID:24040513

Spectral Diversity and Regulation of Coral Fluorescence in a Mesophotic Reef Habitat in the Red Sea

PubMed Central

Eyal, Gal; Wiedenmann, Jörg; Grinblat, Mila; D’Angelo, Cecilia; Kramarsky-Winter, Esti; Treibitz, Tali; Ben-Zvi, Or; Shaked, Yonathan; Smith, Tyler B.; Harii, Saki; Denis, Vianney; Noyes, Tim; Tamir, Raz; Loya, Yossi

2015-01-01

The phenomenon of coral fluorescence in mesophotic reefs, although well described for shallow waters, remains largely unstudied. We found that representatives of many scleractinian species are brightly fluorescent at depths of 50–60 m at the Interuniversity Institute for Marine Sciences (IUI) reef in Eilat, Israel. Some of these fluorescent species have distribution maxima at mesophotic depths (40–100 m). Several individuals from these depths displayed yellow or orange-red fluorescence, the latter being essentially absent in corals from the shallowest parts of this reef. We demonstrate experimentally that in some cases the production of fluorescent pigments is independent of the exposure to light; while in others, the fluorescence signature is altered or lost when the animals are kept in darkness. Furthermore, we show that green-to-red photoconversion of fluorescent pigments mediated by short-wavelength light can occur also at depths where ultraviolet wavelengths are absent from the underwater light field. Intraspecific colour polymorphisms regarding the colour of the tissue fluorescence, common among shallow water corals, were also observed for mesophotic species. Our results suggest that fluorescent pigments in mesophotic reefs fulfil a distinct biological function and offer promising application potential for coral-reef monitoring and biomedical imaging. PMID:26107282

Fluorescence and Spectral Imaging

PubMed Central

DaCosta, Ralph S.; Wilson, Brian C.; Marcon, Norman E.

2007-01-01

Early identification of dysplasia remains a critical goal for diagnostic endoscopy since early discovery directly improves patient survival because it allows endoscopic or surgical intervention with disease localized without lymph node involvement. Clinical studies have successfully used tissue autofluorescence with conventional white light endoscopy and biopsy for detecting adenomatous colonic polyps, differentiating benign hyperplastic from adenomas with acceptable sensitivity and specificity. In Barrett's esophagus, the detection of dysplasia remains problematic because of background inflammation, whereas in the squamous esophagus, autofluorescence imaging appears to be more dependable. Point fluorescence spectroscopy, although playing a crucial role in the pioneering mechanistic development of fluorescence endoscopic imaging, does not seem to have a current function in endoscopy because of its nontargeted sampling and suboptimal sensitivity and specificity. Other point spectroscopic modalities, such as Raman spectroscopy and elastic light scattering, continue to be evaluated in clinical studies, but still suffer the significant disadvantages of being random and nonimaging. A recent addition to the fluorescence endoscopic imaging arsenal is the use of confocal fluorescence endomicroscopy, which provides real-time optical biopsy for the first time. To improve detection of dysplasia in the gastrointestinal tract, a new and exciting development has been the use of exogenous fluorescence contrast probes that specifically target a variety of disease-related cellular biomarkers using conventional fluorescent dyes and novel potent fluorescent nanocrystals (i.e., quantum dots). This is an area of great promise, but still in its infancy, and preclinical studies are currently under way. PMID:18167619

Multi-species Identification of Polymorphic Peptide Variants via Propagation in Spectral Networks

DOE Office of Scientific and Technical Information (OSTI.GOV)

Na, Seungjin; Payne, Samuel H.; Bandeira, Nuno

The spectral networks approach enables the detection of pairs of spectra from related peptides and thus allows for the propagation of annotations from identified peptides to unidentified spectra. Beyond allowing for unbiased discovery of unexpected post-translational modifications, spectral networks are also applicable to multi-species comparative proteomics or metaproteomics to identify numerous orthologous versions of a protein. We present algorithmic and statistical advances in spectral networks that have made it possible to rigorously assess the statistical significance of spectral pairs and accurately estimate the error rate of identifications via propagation. In the analysis of three related Cyanothece species, a model organismmore » for biohydrogen production, spectral networks identified peptides with highly divergent sequences with up to dozens of variants per peptide, including many novel peptides in species that lack a sequenced genome. Furthermore, spectral networks strongly suggested the presence of novel peptides even in genomically characterized species (i.e. missing from databases) in that a significant portion of unidentified multi-species networks included at least two polymorphic peptide variants.« less

An approach to estimate spatial distribution of analyte within cells using spectrally-resolved fluorescence microscopy

NASA Astrophysics Data System (ADS)

Sharma, Dharmendar Kumar; Irfanullah, Mir; Basu, Santanu Kumar; Madhu, Sheri; De, Suman; Jadhav, Sameer; Ravikanth, Mangalampalli; Chowdhury, Arindam

2017-03-01

While fluorescence microscopy has become an essential tool amongst chemists and biologists for the detection of various analyte within cellular environments, non-uniform spatial distribution of sensors within cells often restricts extraction of reliable information on relative abundance of analytes in different subcellular regions. As an alternative to existing sensing methodologies such as ratiometric or FRET imaging, where relative proportion of analyte with respect to the sensor can be obtained within cells, we propose a methodology using spectrally-resolved fluorescence microscopy, via which both the relative abundance of sensor as well as their relative proportion with respect to the analyte can be simultaneously extracted for local subcellular regions. This method is exemplified using a BODIPY sensor, capable of detecting mercury ions within cellular environments, characterized by spectral blue-shift and concurrent enhancement of emission intensity. Spectral emission envelopes collected from sub-microscopic regions allowed us to compare the shift in transition energies as well as integrated emission intensities within various intracellular regions. Construction of a 2D scatter plot using spectral shifts and emission intensities, which depend on the relative amount of analyte with respect to sensor and the approximate local amounts of the probe, respectively, enabled qualitative extraction of relative abundance of analyte in various local regions within a single cell as well as amongst different cells. Although the comparisons remain semi-quantitative, this approach involving analysis of multiple spectral parameters opens up an alternative way to extract spatial distribution of analyte in heterogeneous systems. The proposed method would be especially relevant for fluorescent probes that undergo relatively nominal shift in transition energies compared to their emission bandwidths, which often restricts their usage for quantitative ratiometric imaging in

An approach to estimate spatial distribution of analyte within cells using spectrally-resolved fluorescence microscopy.

PubMed

Sharma, Dharmendar Kumar; Irfanullah, Mir; Basu, Santanu Kumar; Madhu, Sheri; De, Suman; Jadhav, Sameer; Ravikanth, Mangalampalli; Chowdhury, Arindam

2017-01-18

While fluorescence microscopy has become an essential tool amongst chemists and biologists for the detection of various analyte within cellular environments, non-uniform spatial distribution of sensors within cells often restricts extraction of reliable information on relative abundance of analytes in different subcellular regions. As an alternative to existing sensing methodologies such as ratiometric or FRET imaging, where relative proportion of analyte with respect to the sensor can be obtained within cells, we propose a methodology using spectrally-resolved fluorescence microscopy, via which both the relative abundance of sensor as well as their relative proportion with respect to the analyte can be simultaneously extracted for local subcellular regions. This method is exemplified using a BODIPY sensor, capable of detecting mercury ions within cellular environments, characterized by spectral blue-shift and concurrent enhancement of emission intensity. Spectral emission envelopes collected from sub-microscopic regions allowed us to compare the shift in transition energies as well as integrated emission intensities within various intracellular regions. Construction of a 2D scatter plot using spectral shifts and emission intensities, which depend on the relative amount of analyte with respect to sensor and the approximate local amounts of the probe, respectively, enabled qualitative extraction of relative abundance of analyte in various local regions within a single cell as well as amongst different cells. Although the comparisons remain semi-quantitative, this approach involving analysis of multiple spectral parameters opens up an alternative way to extract spatial distribution of analyte in heterogeneous systems. The proposed method would be especially relevant for fluorescent probes that undergo relatively nominal shift in transition energies compared to their emission bandwidths, which often restricts their usage for quantitative ratiometric imaging in

Effects of alcohols on fluorescence intensity and color of a discharged-obelin-based biomarker.

PubMed

Alieva, Roza R; Belogurova, Nadezhda V; Petrova, Alena S; Kudryasheva, Nadezhda S

2014-05-01

Photoproteins are responsible for bioluminescence of marine coelenterates; bioluminescent and fluorescent biomarkers based on photoproteins are useful for monitoring of calcium-dependent processes in medical investigations. Here, we present the analysis of intensity and color of light-induced fluorescence of Ca(2+)-discharged photoprotein obelin in the presence of alcohols (ethanol and glycerol). Complex obelin spectra obtained at different concentrations of the alcohols at 350- and 280-nm excitation (corresponding to polypeptide-bound coelenteramide and tryptophan absorption regions) were deconvoluted into Gaussian components; fluorescent intensity and contributions of the components to experimental spectra were analyzed. Five Gaussian components were found in different spectral regions-ultraviolet (tryptophan emission), blue-green (coelenteramide emission), and red (hypothetical indole-coelenteramide exciplex emission). Inhibition coefficients and contributions of the components to experimental fluorescent spectra showed that presence of alcohols increased contributions of ultraviolet, violet, and red components, but decreased contributions of components in the blue-green region. The effects were related to (1) changes of proton transfer efficiency in fluorescent S*1 state of coelenteramide in the obelin active center and (2) formation of indole-coelenteramide exciplex at 280-nm photoexcitation. The data show that variation of fluorescence color and intensity in the presence of alcohols and dependence of emission spectra on excitation wavelength should be considered while applying the discharged obelin as a fluorescence biomarker.

Forest height Mapping using the fusion of Lidar and MULTI-ANGLE spectral data

NASA Astrophysics Data System (ADS)

Pang, Y.; Li, Z.

2016-12-01

Characterizing the complexity of forest ecosystem over large area is highly complex. Light detection and Ranging (LIDAR) approaches have demonstrated a high capacity to accurately estimate forest structural parameters. A number of satellite mission concepts have been proposed to fuse LiDAR with other optical imagery allowing Multi-angle spectral observations to be captured using the Bidirectional Reflectance Distribution Function (BRDF) characteristics of forests. China is developing the concept of Chinese Terrestrial Carbon Mapping Satellite. A multi-beam waveform Lidar is the main sensor. A multi-angle imagery system is considered as the spatial mapping sensor. In this study, we explore the fusion potential of Lidar and multi-angle spectral data to estimate forest height across different scales. We flew intensive airborne Lidar and Multi-angle hyperspectral data in Genhe Forest Ecological Research Station, Northeast China. Then extended the spatial scale with some long transect flights to cover more forest structures. Forest height data derived from airborne lidar data was used as reference data and the multi-angle hyperspectral data was used as model inputs. Our results demonstrate that the multi-angle spectral data can be used to estimate forest height with the RMSE of 1.1 m with an R2 approximately 0.8.

Ultraviolet and optical spectral morphology of Melnick 42 and Radcliffe 136a in 30 Doradus

NASA Technical Reports Server (NTRS)

Walborn, Nolan R.; Ebbets, Dennis C.; Parker, Joel WM.; Nichols-Bohlin, Joy; White, Richard L.

1992-01-01

HST/GHRS ultraviolet spectrograms of the individual O3 If*/WN6-A object Mk 42 in 30 Dor and the adjacent, central multiple system R136a are compared with each other and with an appropriate sequence of O3 If* and WN6-A standards from the IUE archive. The analogous spectral montages covering the blue-violet regino, based on new, homogeneous, digital observations of the same stars with the CTIO 4 m telescope, are also presented. These comparisons show clearly the intermediate O3/WN nature of the Mk 42 spectrum, in terms of both emission-line strength (increasing with envelope density) and stellar-wind velocity (decreasing with envelope density). It is also shown that R136a possesses stronger WN spectral characteristics than Mk 42, in agreement with HST narrow-band imaging by the WF/PC Team.

Fluorescence lidar multi-color imaging of vegetation

NASA Technical Reports Server (NTRS)

Johansson, J.; Wallinder, E.; Edner, H.; Svanberg, S.

1992-01-01

Multi-color imaging of vegetation fluorescence following laser excitation is reported for distances of 50 m. A mobile laser radar system equipped with a Nd:YAG laser transmitter and a 40 cm diameter telescope was used. Image processing allows extraction of information related to the physiological status of the vegetation and might prove useful in forest decline research.

Ultraviolet-Visible (UV-Vis) and Fluorescence Spectroscopic Investigation of the Interactions of Ionic Liquids and Catalase.

PubMed

Dong, Xing; Fan, Yunchang; Yang, Peng; Kong, Jichuan; Li, Dandan; Miao, Juan; Hua, Shaofeng; Hu, Chaobing

2016-11-01

The inhibitory effects of nine ionic liquids (ILs) on the catalase activity were investigated using fluorescence, absorption ultraviolet-visible spectroscopy. The interactions of ILs and catalase on the molecular level were studied. The experimental results indicated that ILs could inhibit the catalase activity and their inhibitory abilities depended on their chemical structures. Fluorescence experiments showed that hydrogen bonding played an important role in the interaction process. The inhibitory abilities of ILs on catalase activity could be simply described by their hydrophobicity and hydrogen bonding abilities. Unexpected less inhibitory effects of trifluoromethanesulfonate (TfO - ) might be ascribed to its larger size, which makes it difficult to go through the substrate channel of catalase to the active site. © The Author(s) 2016.

Design of a multi-spectral imager built using the compressive sensing single-pixel camera architecture

NASA Astrophysics Data System (ADS)

McMackin, Lenore; Herman, Matthew A.; Weston, Tyler

2016-02-01

We present the design of a multi-spectral imager built using the architecture of the single-pixel camera. The architecture is enabled by the novel sampling theory of compressive sensing implemented optically using the Texas Instruments DLP™ micro-mirror array. The array not only implements spatial modulation necessary for compressive imaging but also provides unique diffractive spectral features that result in a multi-spectral, high-spatial resolution imager design. The new camera design provides multi-spectral imagery in a wavelength range that extends from the visible to the shortwave infrared without reduction in spatial resolution. In addition to the compressive imaging spectrometer design, we present a diffractive model of the architecture that allows us to predict a variety of detailed functional spatial and spectral design features. We present modeling results, architectural design and experimental results that prove the concept.

Spectral characterization of biological aerosol particles using two-wavelength excited laser-induced fluorescence and elastic scattering measurements.

PubMed

Sivaprakasam, Vasanthi; Lin, Horn-Bond; Huston, Alan L; Eversole, Jay D

2011-03-28

A two-wavelength laser-induced fluorescence (LIF) instrument has been developed and used to characterize individual biological aerosol particles, including biological warfare (BW) agent surrogates. Fluorescence in discrete spectral bands from widely different species, and also from similar species under different growth conditions were measured and compared. The two-wavelength excitation approach was found to increase discrimination among several biological materials, and especially with respect to diesel exhaust particles, a common interferent for LIF BW detection systems. The spectral characteristics of a variety of biological materials and ambient air components have been studied as a function of aerosol particle size and incident fluence.

Biochemical Detection and Identification False Alarm Rate Dependence on Wavelength Using Laser Induced Fluorescence

NASA Technical Reports Server (NTRS)

Bhartia, R.; Hug, W. F.; Sala, E. C.; Sijapati, K.; Lane, A. L.; Reid, R. D.; Conrad, P. G.

2006-01-01

Most organic and many inorganic materials absorb strongly in specific wavelength ranges in the deep UV between about 220nm and 300nm. Excitation within these absorption bands results in native fluorescence emission. Each compound or composite material, such as a bacterial spore, has a unique excitation-emission fingerprint that can be used to provide information about the material. The sensitivity and specificity with which these materials can be detected and identified depends on the excitation wavelength and the number and location of observation wavelengths.We will present data on our deep ultraviolet Targeted Ultraviolet Chemical Sensors that demonstrate the sensitivity and specificity of the sensors. In particular, we will demonstrate the ability to quantitatively differentiate a wide range of biochemical agent targets against a wide range of background materials. We will describe the relationship between spectral resolution and specificity in target identification, as well as simple, fast, algorithms to identify materials.Hand-held, battery operated instruments using a deep UV laser and multi-band detection have been developed and deployed on missions to the Antarctic, the Arctic, and the deep ocean with the capability of detecting a single bacterial spore and to differentiate a wide range of organic and biological compounds.

GAMA/H-ATLAS: the ultraviolet spectral slope and obscuration in galaxies

NASA Astrophysics Data System (ADS)

Wijesinghe, D. B.; da Cunha, E.; Hopkins, A. M.; Dunne, L.; Sharp, R.; Gunawardhana, M.; Brough, S.; Sadler, E. M.; Driver, S.; Baldry, I.; Bamford, S.; Liske, J.; Loveday, J.; Norberg, P.; Peacock, J.; Popescu, C. C.; Tuffs, R.; Andrae, E.; Auld, R.; Baes, M.; Bland-Hawthorn, J.; Buttiglione, S.; Cava, A.; Cameron, E.; Conselice, C. J.; Cooray, A.; Croom, S.; Dariush, A.; Dezotti, G.; Dye, S.; Eales, S.; Frenk, C.; Fritz, J.; Hill, D.; Hopwood, R.; Ibar, E.; Ivison, R.; Jarvis, M.; Jones, D. H.; van Kampen, E.; Kelvin, L.; Kuijken, K.; Maddox, S. J.; Madore, B.; Michałowski, M. J.; Nichol, B.; Parkinson, H.; Pascale, E.; Pimbblet, K. A.; Pohlen, M.; Prescott, M.; Rhodighiero, G.; Robotham, A. S. G.; Rigby, E. E.; Seibert, M.; Sergeant, S.; Smith, D. J. B.; Temi, P.; Sutherland, W.; Taylor, E.; Thomas, D.; van der Werf, P.

2011-08-01

We use multiwavelength data from the Galaxy And Mass Assembly (GAMA) and Herschel-ATLAS (H-ATLAS) surveys to compare the relationship between various dust obscuration measures in galaxies. We explore the connections between the ultraviolet (UV) spectral slope, β, the Balmer decrement and the far-infrared (FIR) to 150 nm far-ultraviolet (FUV) luminosity ratio. We explore trends with galaxy mass, star formation rate (SFR) and redshift in order to identify possible systematics in these various measures. We reiterate the finding of other authors that there is a large scatter between the Balmer decrement and the β parameter, and that β may be poorly constrained when derived from only two broad passbands in the UV. We also emphasize that FUV-derived SFRs, corrected for dust obscuration using β, will be overestimated unless a modified relation between β and the attenuation factor is used. Even in the optimum case, the resulting SFRs have a significant scatter, well over an order of magnitude. While there is a stronger correlation between the IR-to-FUV luminosity ratio and β parameter than with the Balmer decrement, neither of these correlations are particularly tight, and dust corrections based on β for high-redshift galaxy SFRs must be treated with caution. We conclude with a description of the extent to which the different obscuration measures are consistent with each other as well as the effects of including other galactic properties on these correlations.

The ultraviolet bands of the CO2/plus/ ion in comets

NASA Astrophysics Data System (ADS)

Festou, M. C.; Feldman, P. D.; Weaver, H. A.

1982-05-01

Eight comets are studied with the International Ultraviolet Explorer spectrographs. The existence of the CO2(plus) ion in a comet is confirmed through the presence of the 2890 A doublet in at least three of these objects. Spatial and spectral resolution obtained in comets Bradfield (1979 X) and Seargent (1978 XV) permit a discussion of the production mechanisms of this ion. The spectra reveal new ionic features in the 3100-3400 A range, which are attributed to resonance fluorescence of the Fox-Duffendack-Barker system of the CO2(plus) ion and, near 3350 A, to the OH(plus) ion.

Interaction of the minocycline with extracelluar protein and intracellular protein by multi-spectral techniques and molecular docking

NASA Astrophysics Data System (ADS)

Fang, Qing; Wang, Yirun; Hu, Taoying; Liu, Ying

2017-02-01

The interaction of minocyeline (MNC) with extracelluar protein (lysozyme, LYSO) or intracellular protein (bovine hemoglobin, BHb) was investigated using multi-spectral techniques and molecular docking in vitro. Fluorescence studies suggested that MNC quenched LYSO/BHb fluorescence in a static mode with binding constants of 2.01 and 0.26 × 104 L•mol-1 at 298 K, respectively. The LYZO-MNC system was more easily influenced by temperature (298 and 310 K) than the BHb-MNC system. The thermodynamic parameters demonstrated that hydrogen bonds and van der Waals forces played the major role in the binding process. Based on the Förster theory of nonradiative energy transfer, the binding distances between MNC and the inner tryptophan residues of LYSO and BHb were calculated to be 4.34 and 3.49 nm, respectively. Furthermore, circular dichroism spectra (CD), Fourier transforms infrared (FTIR), UV-vis, and three-dimensional fluorescence spectra results indicated the secondary structures of LYSO and BHb were partially destroyed by MNC with the α-helix percentage of LYZO-MNC increased (17.8-28.6%) while that of BHb-MNC was decreased (41.6-39.6%). UV-vis spectral results showed these binding interactions could cause conformational and some micro-environmental changes of LYSO and BHb. In accordance with the results of molecular docking, In LYZO-MNC system, MNC was mainly bound in the active site hinge region where Trp-62 and Trp-63 are located, and in MNC-BHb system, MNC was close to the subunit α 1 of BHb, molecular docking analysis supported the thermodynamic results well. The work contributes to clarify the mechanism of MNC with two proteins at molecular level.

The 1997 North American Interagency Intercomparison of Ultraviolet Spectroradiometers Including Narrowband Filter Radiometers

PubMed Central

Lantz, Kathleen; Disterhoft, Patrick; Early, Edward; Thompson, Ambler; DeLuisi, John; Berndt, Jerry; Harrison, Lee; Kiedron, Peter; Ehramjian, James; Bernhard, Germar; Cabasug, Lauriana; Robertson, James; Mou, Wanfeng; Taylor, Thomas; Slusser, James; Bigelow, David; Durham, Bill; Janson, George; Hayes, Douglass; Beaubien, Mark; Beaubien, Arthur

2002-01-01

The fourth North American Intercomparison of Ultraviolet Monitoring Spectroradiometers was held September 15 to 25, 1997 at Table Mountain outside of Boulder, Colorado, USA. Concern over stratospheric ozone depletion has prompted several government agencies in North America to establish networks of spectroradiometers for monitoring solar ultraviolet irradiance at the surface of the Earth. The main purpose of the Intercomparison was to assess the ability of spectroradiometers to accurately measure solar ultraviolet irradiance, and to compare the results between instruments of different monitoring networks. This Intercomparison was coordinated by NIST and NOAA, and included participants from the ASRC, EPA, NIST, NSF, SERC, USDA, and YES. The UV measuring instruments included scanning spectroradiometers, spectrographs, narrow band multi-filter radiometers, and broadband radiometers. Instruments were characterized for wavelength accuracy, bandwidth, stray-light rejection, and spectral irradiance responsivity. The spectral irradiance responsivity was determined two to three times outdoors to assess temporal stability. Synchronized spectral scans of the solar irradiance were performed over several days. Using the spectral irradiance responsivities determined with the NIST traceable standard lamp, and a simple convolution technique with a Gaussian slit-scattering function to account for the different bandwidths of the instruments, the measured solar irradiance from the spectroradiometers excluding the filter radiometers at 16.5 h UTC had a relative standard deviation of ±4 % for wavelengths greater than 305 nm. The relative standard deviation for the solar irradiance at 16.5 h UTC including the filter radiometer was ±4 % for filter functions above 300 nm. PMID:27446717

Multi-Centrality Graph Spectral Decompositions and Their Application to Cyber Intrusion Detection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Pin-Yu; Choudhury, Sutanay; Hero, Alfred

Many modern datasets can be represented as graphs and hence spectral decompositions such as graph principal component analysis (PCA) can be useful. Distinct from previous graph decomposition approaches based on subspace projection of a single topological feature, e.g., the centered graph adjacency matrix (graph Laplacian), we propose spectral decomposition approaches to graph PCA and graph dictionary learning that integrate multiple features, including graph walk statistics, centrality measures and graph distances to reference nodes. In this paper we propose a new PCA method for single graph analysis, called multi-centrality graph PCA (MC-GPCA), and a new dictionary learning method for ensembles ofmore » graphs, called multi-centrality graph dictionary learning (MC-GDL), both based on spectral decomposition of multi-centrality matrices. As an application to cyber intrusion detection, MC-GPCA can be an effective indicator of anomalous connectivity pattern and MC-GDL can provide discriminative basis for attack classification.« less

Spectrally resolved fluorescence lifetime imaging to investigate cell metabolism in malignant and nonmalignant oral mucosa cells

NASA Astrophysics Data System (ADS)

Rück, Angelika; Hauser, Carmen; Mosch, Simone; Kalinina, Sviatlana

2014-09-01

Fluorescence-guided diagnosis of tumor tissue is in many cases insufficient, because false positive results interfere with the outcome. Improvement through observation of cell metabolism might offer the solution, but needs a detailed understanding of the origin of autofluorescence. With respect to this, spectrally resolved multiphoton fluorescence lifetime imaging was investigated to analyze cell metabolism in metabolic phenotypes of malignant and nonmalignant oral mucosa cells. The time-resolved fluorescence characteristics of NADH were measured in cells of different origins. The fluorescence lifetime of bound and free NADH was calculated from biexponential fitting of the fluorescence intensity decay within different spectral regions. The mean lifetime was increased from nonmalignant oral mucosa cells to different squamous carcinoma cells, where the most aggressive cells showed the longest lifetime. In correlation with reports in the literature, the total amount of NADH seemed to be less for the carcinoma cells and the ratio of free/bound NADH was decreased from nonmalignant to squamous carcinoma cells. Moreover for squamous carcinoma cells a high concentration of bound NADH was found in cytoplasmic organelles (mainly mitochondria). This all together indicates that oxidative phosphorylation and a high redox potential play an important role in the energy metabolism of these cells.

Spectral gain profile of a multi-stack terahertz quantum cascade laser

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bachmann, D., E-mail: dominic.bachmann@tuwien.ac.at; Deutsch, C.; Krall, M.

2014-11-03

The spectral gain of a multi-stack terahertz quantum cascade laser, composed of three active regions with emission frequencies centered at 2.3, 2.7, and 3.0 THz, is studied as a function of driving current and temperature using terahertz time-domain spectroscopy. The optical gain associated with the particular quantum cascade stacks clamps at different driving currents and saturates to different values. We attribute these observations to varying pumping efficiencies of the respective upper laser states and to frequency dependent optical losses. The multi-stack active region exhibits a spectral gain full width at half-maximum of 1.1 THz. Bandwidth and spectral position of themore » measured gain match with the broadband laser emission. As the laser action ceases with increasing operating temperature, the gain at the dominant lasing frequency of 2.65 THz degrades sharply.« less

Avalanche multiplication in AlGaN-based heterostructures for the ultraviolet spectral range

NASA Astrophysics Data System (ADS)

Hahn, L.; Fuchs, F.; Kirste, L.; Driad, R.; Rutz, F.; Passow, T.; Köhler, K.; Rehm, R.; Ambacher, O.

2018-04-01

AlxGa1-xN based avalanche photodiodes grown on sapphire substrate with Al-contents of x = 0.65 and x = 0.60 have been examined under back- and frontside illumination with respect to their avalanche gain properties. The photodetectors suitable for the solar-blind ultraviolet spectral regime show avalanche gain for voltages in excess of 30 V reverse bias in the linear gain mode. Devices with a mesa diameter of 100 μm exhibit stable avalanche gain below the break through threshold voltage, exceeding a multiplication gain of 5500 at 84 V reverse bias. A dark current below 1 pA can be found for reverse voltages up to 60 V.

Investigation of ultraviolet fluxes of normal and peculiar stars

NASA Technical Reports Server (NTRS)

Deutschman, W. A.; Schild, R. E.

1974-01-01

Data from Project Celescope, a program that photographed the ultraviolet sky, in order to study several problems in current astrophysics are analyzed. Two star clusters, the Pleiades and the Hyades, reveal differences between the two that we are unable to explain simply from their differences in chemical abundance, rotation, or reddening. Data for Orion show large scatter, which appears to be in the sense that the Orion stars are too faint for their ground-based photometry. Similarly, many supergiants in the association Sco OB1 are too faint in the ultraviolet, but the ultraviolet brightness appears to be only poorly correlated with spectral type. Ultraviolet Celescope data for several groups of peculiar stars have also been analyzed. The strong He I stars are too faint in the ultraviolet, possibly owing to enhancement of O II continuous opacity due to oxygen overabundance. The Be stars appear to have ultraviolet colors normal for their MK spectral types. The P Cygni stars are considerably fainter than main-sequence stars of comparable spectral type, probably owing, at least in part, to line blocking by resonance lines of multiply ionized light metals. The Wolf-Rayet stars have ultraviolet color temperatures of O stars.

SCOUSE: Semi-automated multi-COmponent Universal Spectral-line fitting Engine

NASA Astrophysics Data System (ADS)

Henshaw, J. D.; Longmore, S. N.; Kruijssen, J. M. D.; Davies, B.; Bally, J.; Barnes, A.; Battersby, C.; Burton, M.; Cunningham, M. R.; Dale, J. E.; Ginsburg, A.; Immer, K.; Jones, P. A.; Kendrew, S.; Mills, E. A. C.; Molinari, S.; Moore, T. J. T.; Ott, J.; Pillai, T.; Rathborne, J.; Schilke, P.; Schmiedeke, A.; Testi, L.; Walker, D.; Walsh, A.; Zhang, Q.

2016-01-01

The Semi-automated multi-COmponent Universal Spectral-line fitting Engine (SCOUSE) is a spectral line fitting algorithm that fits Gaussian files to spectral line emission. It identifies the spatial area over which to fit the data and generates a grid of spectral averaging areas (SAAs). The spatially averaged spectra are fitted according to user-provided tolerance levels, and the best fit is selected using the Akaike Information Criterion, which weights the chisq of a best-fitting solution according to the number of free-parameters. A more detailed inspection of the spectra can be performed to improve the fit through an iterative process, after which SCOUSE integrates the new solutions into the solution file.

The difficulty of ultraviolet emssion from supernovae

NASA Technical Reports Server (NTRS)

Colgate, S. A.

1971-01-01

There are certain conceptual difficulties in the theory of the generation of ultraviolet radiation which is presumed for the creation of the optical fluorescence mechanism of supernova light emission and ionization of a nebula as large as the Gum nebula. Requirements concerning the energy distribution of the ultraviolet photons are: 1) The energy of the greater part of the photons must be sufficient to cause both helium fluorescence and hydrogen ionization. 2) If the photons are emitted in an approximate black body spectrum, the fraction of energy emitted in the optical must be no more than what is already observed. Ultraviolet black body emission depends primarily on the energy source. The probability that the wide mixture of elements present in the interstellar medium and supernova ejecta results in an emission localized in a limited region with less than 0.001 emission in the visible, for either ionization or fluorescence ultraviolet, is remote. Therefore transparent emission must be excluded as unlikely, and black body or at least quasi-black-body emission is more probable.

Exploring the Origin of Blue and Ultraviolet Fluorescence in Graphene Oxide.

PubMed

Kozawa, Daichi; Miyauchi, Yuhei; Mouri, Shinichiro; Matsuda, Kazunari

2013-06-20

We studied the fluorescence (FL) properties of highly exfoliated graphene oxide (GO) in aqueous solution using continuous-wave and time-resolved FL spectroscopy. The FL spectra of highly exfoliated GO showed two distinct peaks at ∼440 (blue) and ∼300 nm [ultraviolet (UV)]. The FL of GO in the UV region at ∼300 nm was observed for the first time. The average FL lifetimes of the emission peaks at ∼440 and ∼300 nm are 8-13 and 6-8 ns, respectively. The experimentally observed peak wavelengths of pH-dependent FL, FL excitation spectra, and the FL lifetimes are nearly coincident with those of aromatic compounds bound with oxygen functional groups, which suggests that the FL comes from sp(2) fragments consisting of small numbers of aromatic rings with oxygen functional groups acting as FL centers in the GO.

Norcyanine dyes with benzo[c,d]indolium moiety: Spectral sensitivity with pH change for fluorescence pH imaging in living cells.

PubMed

Guan, Li; Liu, Qi; Zhang, Borui; Wang, Lanying

2017-01-01

Fluorescence pH imaging in living cells is a rapidly expanding research direction, however, it relies on the development of pH-sensitive fluorescent imaging agents. Here four norcyanine dyes with benzo[c,d]indolium moiety, exhibiting high spectral sensitivity with pH changes, were synthesized for fluorescence pH imaging in living cells, and characterized by 1 H NMR, 13 C NMR, IR, UV-Vis and HRMS. The investigation of their spectral properties in methanol and water showed that the absorption and emission maxima were in the region 488-618nm and 583-651nm, respectively, and four dyes exhibited high photostability. The pH spectral titrations showed that selective dye D1 had pH-dependent absorption spectral changes within the pH range of 2.4 to 9.4, and high fluorescent spectral sensitivity at pH5.0-8.0, with a pK a of 5.0. A cell association study indicated that dye D1 exhibited no or mild cytotoxicity at the application dose and duration, and could be accumulated in cells and mainly distributed in the cytoplasm, giving red fluorescence imaging. In particular, dye D1 could achieve pH-dependent fluorescence imaging in living cells with the increase of pH from 3.0 to 8.0, at excitation wavelength of 543nm and receiving wavelength of 655-755nm, which was valuable for studying the weak acidic, neutral and weak alkaline biological tissue compartments. Copyright © 2016 Elsevier B.V. All rights reserved.

Peculiarities of the statistics of spectrally selected fluorescence radiation in laser-pumped dye-doped random media

NASA Astrophysics Data System (ADS)

Yuvchenko, S. A.; Ushakova, E. V.; Pavlova, M. V.; Alonova, M. V.; Zimnyakov, D. A.

2018-04-01

We consider the practical realization of a new optical probe method of the random media which is defined as the reference-free path length interferometry with the intensity moments analysis. A peculiarity in the statistics of the spectrally selected fluorescence radiation in laser-pumped dye-doped random medium is discussed. Previously established correlations between the second- and the third-order moments of the intensity fluctuations in the random interference patterns, the coherence function of the probe radiation, and the path difference probability density for the interfering partial waves in the medium are confirmed. The correlations were verified using the statistical analysis of the spectrally selected fluorescence radiation emitted by a laser-pumped dye-doped random medium. Water solution of Rhodamine 6G was applied as the doping fluorescent agent for the ensembles of the densely packed silica grains, which were pumped by the 532 nm radiation of a solid state laser. The spectrum of the mean path length for a random medium was reconstructed.

An improved feature extraction algorithm based on KAZE for multi-spectral image

NASA Astrophysics Data System (ADS)

Yang, Jianping; Li, Jun

2018-02-01

Multi-spectral image contains abundant spectral information, which is widely used in all fields like resource exploration, meteorological observation and modern military. Image preprocessing, such as image feature extraction and matching, is indispensable while dealing with multi-spectral remote sensing image. Although the feature matching algorithm based on linear scale such as SIFT and SURF performs strong on robustness, the local accuracy cannot be guaranteed. Therefore, this paper proposes an improved KAZE algorithm, which is based on nonlinear scale, to raise the number of feature and to enhance the matching rate by using the adjusted-cosine vector. The experiment result shows that the number of feature and the matching rate of the improved KAZE are remarkably than the original KAZE algorithm.

Fluorescence lifetime imaging and Fourier transform infrared spectroscopy of Michelangelo's David.

PubMed

Comelli, Daniela; Valentini, Gianluca; Cubeddu, Rinaldo; Toniolo, Lucia

2005-09-01

We developed a combined procedure for the analysis of works of art based on a portable system for fluorescence imaging integrated with analytical measurements on microsamples. The method allows us to localize and identify organic and inorganic compounds present on the surface of artworks. The fluorescence apparatus measures the temporal and spectral features of the fluorescence emission, excited by ultraviolet (UV) laser pulses. The kinetic of the emission is studied through a fluorescence lifetime imaging system, while an optical multichannel analyzer measures the fluorescence spectra of selected points. The chemical characterization of the compounds present on the artistic surfaces is then performed by means of analytical measurements on microsamples collected with the assistance of the fluorescence maps. The previous concepts have been successfully applied to study the contaminants on the surface of Michelangelo's David. The fluorescence analysis combined with Fourier transform infrared (FT-IR) measurements revealed the presence of beeswax, which permeates most of the statue surface, and calcium oxalate deposits mainly arranged in vertical patterns and related to rain washing.

Multi-spectral optical scanners for commercial earth observation missions

NASA Astrophysics Data System (ADS)

Schröter, Karin; Engel, Wolfgang; Berndt, Klaus

2017-11-01

In recent years, a number of commercial Earth observation missions have been initiated with the aim to gather data in the visible and near-infrared wavelength range. Some of these missions aim at medium resolution (5 to 10 m) multi-spectral imaging with the special background of daily revisiting. Typical applications aim at monitoring of farming area for growth control and harvest prediction, irrigation control, or disaster monitoring such as hail damage in farming, or flood survey. In order to arrive at profitable business plans for such missions, it is mandatory to establish the space segment, i.e. the spacecraft with their opto -electronic payloads, at minimum cost while guaranteeing maximum reliability for mission success. As multiple spacecraft are required for daily revisiting, the solutions are typically based on micro-satellites. This paper presents designs for multi-spectral opto-electric scanners for this type of missions. These designs are drive n by minimum mass and power budgets of microsatellites, and the need for minimum cost. As a consequence, it is mandatory to arrive at thermally robust, compact telescope designs. The paper gives a comparison between refractive, catadioptric, and TMA optics. For mirror designs, aluminium and Zerodur mirror technologies are briefly discussed. State-of-the art focal plane designs are presented. The paper also addresses the choice of detector technologies such as CCDs and CMOS Active Pixel Sensors. The electronics of the multi-spectral scanners represent the main design driver regarding power consumption, reliability, and (most often) cost. It can be subdivided into the detector drive electronics, analog and digital data processing chains, the data mass memory unit, formatting and down - linking units, payload control electronics, and local power supply. The paper gives overviews and trade-offs between data compression strategies and electronics solutions, mass memory unit designs, and data formatting approaches

Global analysis of microscopic fluorescence lifetime images using spectral segmentation and a digital micromirror spatial illuminator.

PubMed

Bednarkiewicz, Artur; Whelan, Maurice P

2008-01-01

Fluorescence lifetime imaging (FLIM) is very demanding from a technical and computational perspective, and the output is usually a compromise between acquisition/processing time and data accuracy and precision. We present a new approach to acquisition, analysis, and reconstruction of microscopic FLIM images by employing a digital micromirror device (DMD) as a spatial illuminator. In the first step, the whole field fluorescence image is collected by a color charge-coupled device (CCD) camera. Further qualitative spectral analysis and sample segmentation are performed to spatially distinguish between spectrally different regions on the sample. Next, the fluorescence of the sample is excited segment by segment, and fluorescence lifetimes are acquired with a photon counting technique. FLIM image reconstruction is performed by either raster scanning the sample or by directly accessing specific regions of interest. The unique features of the DMD illuminator allow the rapid on-line measurement of global good initial parameters (GIP), which are supplied to the first iteration of the fitting algorithm. As a consequence, a decrease of the computation time required to obtain a satisfactory quality-of-fit is achieved without compromising the accuracy and precision of the lifetime measurements.

Multi-spectral fiber spectroscopy in 0,4-16μm range for biomedical applications(Conference Presentation)

NASA Astrophysics Data System (ADS)

Artyushenko, Viacheslav

2017-02-01

Various biomedical applications of fiber optics in a broad spectral range 0,4-16μm span from endoscopic imaging and Photo Dynamic Diagnostics (PDD) to laser power delivery for minimal invasive laser surgery, tissue coagulation and welding, Photo Dynamic Therapy (PDT), etc. Present review will highlight the latest results in advanced fiber solutions for a precise tissue diagnostics and control of some therapy methods - for so called "theranostic". Spectral fiber sensing for label free analysis of tissue composition helps to differentiate malignant and normal tissue to secure minimal invasive, but complete tumor removal or treatment. All key methods of Raman, fluorescence, diffuse reflection & MIR-absorption spectroscopy will be compared when used for the same spot of tissue - to select the most specific, sensitive and accurate method or to combine them for the synergy enhanced effect. The most informative spectral features for distinct organs/ tumor can be used to design special fiber sensors to be developed for portable and low cost applications with modern IT-features. Examples of multi-spectral tissue diagnostics promising for the future clinical applications will be presented to enable reduced mortality from cancer in the future. Translation of described methods into clinical practice will be discussed in comparison with the other method of optical diagnostics which should enhance modern medicine by less invasive, more precise and more effective methods of therapy to be fused with in-vivo diagnostics sensors & systems.

Fluorescence spectral shift of QD films with electron injection: Dependence on counterion proximity

NASA Astrophysics Data System (ADS)

Lu, Meilin; Li, Bo; Zhang, Yaxin; Liu, Weilong; Yang, Yanqiang; Wang, Yuxiao; Yang, Qingxin

2017-05-01

Due to the promising application of quantum dot (QD) films in solar cells, LEDs and environmental detectors, the fluorescence of charged QD films has achieved much attention during recent years. In this work, we observe the spectral shift of photoluminescence (PL) in charged CdSe/ZnS QD films controlled by electrochemical potential. The spectral center under negative bias changes from red-shift to blue-shift while introducing smaller inorganic counterions (potassium ions) into the electrolyte. This repeatable effect is attributed to the enhanced electron injection with smaller cations and the electronic perturbations of QD luminescence by these excess charges.

Experimental feasibility of the airborne measurement of absolute oil fluorescence spectral conversion efficiency

NASA Technical Reports Server (NTRS)

Hoge, F. E.; Swift, R. N.

1983-01-01

Airborne lidar oil spill experiments carried out to determine the practicability of the AOFSCE (absolute oil fluorescence spectral conversion efficiency) computational model are described. The results reveal that the model is suitable over a considerable range of oil film thicknesses provided the fluorescence efficiency of the oil does not approach the minimum detection sensitivity limitations of the lidar system. Separate airborne lidar experiments to demonstrate measurement of the water column Raman conversion efficiency are also conducted to ascertain the ultimate feasibility of converting such relative oil fluorescence to absolute values. Whereas the AOFSCE model is seen as highly promising, further airborne water column Raman conversion efficiency experiments with improved temporal or depth-resolved waveform calibration and software deconvolution techniques are thought necessary for a final determination of suitability.

A study on the interaction of horse heart cytochrome c with some conventional and ionic liquid surfactants probed by ultraviolet-visible and fluorescence spectroscopic techniques

NASA Astrophysics Data System (ADS)

Mondal, Satyajit; Das, Bijan

2018-06-01

The interactions of a protein cytochrome c with some selected conventional and ionic liquid surfactants have been investigated at pH 7.4 using ultraviolet-visible and fluorescence spectroscopic techniques. We used four conventional surfactants - cetyltrimethylammonium bromide (CTAB), dodecyltrimethylammonium bromide (DTAB), sodium N-dodecanoylsarcosinate (SDDS), and N-decanoyl-N-methylglucamine (Mega 10), and a surface active ionic liquid 1-hexadecyl-3-methylimidazolium chloride (C16MeImCl). All the investigated surfactants were found to induce an unfolding of the protein cytochrome c. In presence of CTAB, SDDS and C16MeImCl, the heme iron atom was found to loose methionine from its axial position. Differential binding of the surfactant monomers and their micelles to the protein molecules was inferred. The ionic surfactants were found to be more effective than the nonionic one in unfolding the investigated protein. However, the extent of binding of CTAB/C16MeImCl to cytochrome c reaches a plateau past the critical micellization concentration (cmc) of the surfactant. For each of the cytochrome c-DTAB, cytochrome c-SDDS and cytochrome c-Mega 10 system, although there exists an inflection in the surfactant-binding, saturation point could not be detected. It has been demonstrated from the ultraviolet-visible spectral studies that the oxidation state of iron in cytochrome c does not change when the protein binds with the investigated surfactants.

Hyperspectral fluorescence imaging with multi wavelength LED excitation

NASA Astrophysics Data System (ADS)

Luthman, A. Siri; Dumitru, Sebastian; Quirós-Gonzalez, Isabel; Bohndiek, Sarah E.

2016-04-01

Hyperspectral imaging (HSI) can combine morphological and molecular information, yielding potential for real-time and high throughput multiplexed fluorescent contrast agent imaging. Multiplexed readout from targets, such as cell surface receptors overexpressed in cancer cells, could improve both sensitivity and specificity of tumor identification. There remains, however, a need for compact and cost effective implementations of the technology. We have implemented a low-cost wide-field multiplexed fluorescence imaging system, which combines LED excitation at 590, 655 and 740 nm with a compact commercial solid state HSI system operating in the range 600 - 1000 nm. A key challenge for using reflectance-based HSI is the separation of contrast agent fluorescence from the reflectance of the excitation light. Here, we illustrate how it is possible to address this challenge in software, using two offline reflectance removal methods, prior to least-squares spectral unmixing. We made a quantitative comparison of the methods using data acquired from dilutions of contrast agents prepared in well-plates. We then established the capability of our HSI system for non-invasive in vivo fluorescence imaging in small animals using the optimal reflectance removal method. The HSI presented here enables quantitative unmixing of at least four fluorescent contrast agents (Alexa Fluor 610, 647, 700 and 750) simultaneously in living mice. A successful unmixing of the four fluorescent contrast agents was possible both using the pure contrast agents and with mixtures. The system could in principle also be applied to imaging of ex vivo tissue or intraoperative imaging in a clinical setting. These data suggest a promising approach for developing clinical applications of HSI based on multiplexed fluorescence contrast agent imaging.

Initial clinical testing of a multi-spectral imaging system built on a smartphone platform

NASA Astrophysics Data System (ADS)

Mink, Jonah W.; Wexler, Shraga; Bolton, Frank J.; Hummel, Charles; Kahn, Bruce S.; Levitz, David

2016-03-01

Multi-spectral imaging systems are often expensive and bulky. An innovative multi-spectral imaging system was fitted onto a mobile colposcope, an imaging system built around a smartphone in order to image the uterine cervix from outside the body. The multi-spectral mobile colposcope (MSMC) acquires images at different wavelengths. This paper presents the clinical testing of MSMC imaging (technical validation of the MSMC system is described elsewhere 1 ). Patients who were referred to colposcopy following abnormal screening test (Pap or HPV DNA test) according to the standard of care were enrolled. Multi-spectral image sets of the cervix were acquired, consisting of images from the various wavelengths. Image acquisition took 1-2 sec. Areas suspected for dysplasia under white light imaging were biopsied, according to the standard of care. Biopsied sites were recorded on a clockface map of the cervix. Following the procedure, MSMC data was processed from the sites of biopsied sites. To date, the initial histopathological results are still outstanding. Qualitatively, structures in the cervical images were sharper at lower wavelengths than higher wavelengths. Patients tolerated imaging well. The result suggests MSMC holds promise for cervical imaging.

Field Studies of Broadband Aerosol Optical Extinction in the Ultraviolet Spectral Region

NASA Astrophysics Data System (ADS)

Washenfelder, R. A.; Attwood, A.; Brock, C. A.; Brown, S. S.

2013-12-01

Aerosols influence the Earth's radiative budget by scattering and absorbing incoming solar radiation. The optical properties of aerosols vary as a function of wavelength, but few measurements have reported the wavelength dependence of aerosol extinction cross sections and complex refractive indices. In the case of brown carbon, its wavelength-dependent absorption in the ultraviolet spectral region has been suggested as an important component of aerosol radiative forcing. We describe a new field instrument to measure aerosol optical extinction as a function of wavelength, using cavity enhanced spectroscopy with a broadband light source. The instrument consists of two broadband channels which span the 360-390 and 385-420 nm spectral regions using two light emitting diodes (LED) and a grating spectrometer with charge-coupled device (CCD) detector. We deployed this instrument during the Fire Lab at Missoula Experiment during Fall 2012 to measure biomass burning aerosol, and again during the Southern Oxidant and Aerosol Study in summer 2013 to measure organic aerosol in the Southeastern U.S. In both field experiments, we determined aerosol optical extinction as a function of wavelength and can interpret this together with size distribution and composition measurements to characterize the aerosol optical properties and radiative forcing.

[Spectral diversity among the members of the family of Green Fluorescent Protein in hydroid jellyfish (Cnidaria, Hydrozoa)].

PubMed

Ianushevich, Iu G; Shagin, D A; Fradkov, A F; Shakhbazov, K S; Barsova, E V; Gurskaia, N G; Labas, Iu A; Matts, M V; Luk'ianov, k A; Lul'ianov, S A

2005-01-01

The cDNAs encoding the genes of new proteins homologous to the well-known Green Fluorescent Protein (GFP) from the hydroid jellyfish Aequorea victoria were cloned. Two green fluorescent proteins from one un-identified anthojellyfish, a yellow fluorescent protein from Phialidium sp., and a nonfluorescent chromoprotein from another unidentified anthojellyfish were characterized. Thus, a broad diversity of GFP-like proteins among the organisms of the class Hydrozoa in both spectral properties and primary structure was shown.

Fluorescence observations of LDEF exposed materials as an indicator of induced material reactions

NASA Technical Reports Server (NTRS)

Linton, Roger C.; Whitaker, Ann F.; Kamenetzky, Rachel R.

1993-01-01

Observations and measurements of induced changes in the fluorescent emission of materials exposed to the space environment on the Long Duration Exposure Facility (LDEF) have revealed systematic patterns of material-dependent behavior. These results have been supplemented by inspection of similar materials exposed on previous Space Shuttle Missions and in laboratory testing. The space environmental factors affecting the fluorescence of exposed materials have been found to include (but are not necessarily limited to) solar ultraviolet (UV) radiation, atomic oxygen (AO), thermal vacuum exposure, and synergistic combinations of these factors. Observed changes in material fluorescent behavior include stimulation, quenching, and spectral band shifts of emission. For example, the intrinsic yellow fluorescence of zinc oxide pigmented thermal control coatings undergoes quenching as a result of exposure, while coloration is stimulated in the fluorescent emission of several polyurethane coating materials. The changes in fluorescent behavior of these materials are shown to be a revealing indicator of induced material reactions as a result of space environmental exposure.

Spectral Interferences Manganese (Mn) - Europium (Eu) Lines in X-Ray Fluorescence Spectrometry Spectrum

NASA Astrophysics Data System (ADS)

Tanc, Beril; Kaya, Mustafa; Gumus, Lokman; Kumral, Mustafa

2016-04-01

X-ray fluorescence (XRF) spectrometry is widely used for quantitative and semi quantitative analysis of many major, minor and trace elements in geological samples. Some advantages of the XRF method are; non-destructive sample preparation, applicability for powder, solid, paste and liquid samples and simple spectrum that are independent from chemical state. On the other hand, there are some disadvantages of the XRF methods such as poor sensitivity for low atomic number elements, matrix effect (physical matrix effects, such as fine versus course grain materials, may impact XRF performance) and interference effect (the spectral lines of elements may overlap distorting results for one or more elements). Especially, spectral interferences are very significant factors for accurate results. In this study, semi-quantitative analyzed manganese (II) oxide (MnO, 99.99%) was examined. Samples were pelleted and analyzed with XRF spectrometry (Bruker S8 Tiger). Unexpected peaks were obtained at the side of the major Mn peaks. Although sample does not contain Eu element, in results 0,3% Eu2O3 was observed. These result can occur high concentration of MnO and proximity of Mn and Eu lines. It can be eliminated by using correction equation or Mn concentration can confirm with other methods (such as Atomic absorption spectroscopy). Keywords: Spectral Interferences; Manganese (Mn); Europium (Eu); X-Ray Fluorescence Spectrometry Spectrum.

The Ultraviolet Spectrum of the Jovian Dayglow

NASA Technical Reports Server (NTRS)

Liu, Weihong; Dalgarno, A.

1995-01-01

The ultraviolet spectra of molecular hydrogen H2 and HD due to solar fluorescence and photoelectron excitation are calculated and compared with the Jovian equatorial dayglow spectrum measured at 3 A resolution at solar maximum. The dayglow emission is accounted for in both brightness and spectral shape by the solar fluorescence and photoelectron excitation and requires no additional energy source. The emission is characterized by an atmospheric temperature of 530 K and an H2 column density of 10(exp 20) cm(exp -2). The dayglow spectrum contains a cascade contribution to the Lyman band emission from high-lying E and F states. Its relative weakness at short wavelengths is due to both self-absorption by H2 and absorption by CH4. Strong wavelength coincidences of solar emission lines and absorption lines of H2 and HD produce unique line spectra which can be identified in the dayglow spectrum. The strongest fluorescence is due to absorption of the solar Lyman-beta line at 1025.72 A by the P(1) line of the (6, 0) Lyman band of H2 at 1025.93 A. The fluorescence lines due to absorption of the solar O 6 line at 1031.91 A by vibrationally excited H2 via the Q(3) line of the (1, 1) Werner band at 1031.86 A are identified. The fluorescence lines provide a sensitive measure of the atmospheric temperature. There occurs an exact coincidence of the solar O 6 line at 1031.91 A and the R(0) line of the (6, 0) Lyman band of HD at 1031-91 A, but HD on Jupiter is difficult to detect due to the dominance of the H2 emission where the HD emission is particularly strong. Higher spectral resolution and higher sensitivity may make possible such a detection. The high resolution (0.3 A) spectra of H2 and HD are presented to stimulate search for the HD on Jupiter with the Hubble Space Telescope.

Multi scales based sparse matrix spectral clustering image segmentation

NASA Astrophysics Data System (ADS)

Liu, Zhongmin; Chen, Zhicai; Li, Zhanming; Hu, Wenjin

2018-04-01

In image segmentation, spectral clustering algorithms have to adopt the appropriate scaling parameter to calculate the similarity matrix between the pixels, which may have a great impact on the clustering result. Moreover, when the number of data instance is large, computational complexity and memory use of the algorithm will greatly increase. To solve these two problems, we proposed a new spectral clustering image segmentation algorithm based on multi scales and sparse matrix. We devised a new feature extraction method at first, then extracted the features of image on different scales, at last, using the feature information to construct sparse similarity matrix which can improve the operation efficiency. Compared with traditional spectral clustering algorithm, image segmentation experimental results show our algorithm have better degree of accuracy and robustness.

Nonlinear spectral imaging of biological tissues

NASA Astrophysics Data System (ADS)

Palero, J. A.

2007-07-01

The work presented in this thesis demonstrates live high resolution 3D imaging of tissue in its native state and environment. The nonlinear interaction between focussed femtosecond light pulses and the biological tissue results in the emission of natural autofluorescence and second-harmonic signal. Because biological intrinsic emission is generally very weak and extends from the ultraviolet to the visible spectral range, a broad-spectral range and high sensitivity 3D spectral imaging system is developed. Imaging the spectral characteristics of the biological intrinsic emission reveals the structure and biochemistry of the cells and extra-cellular components. By using different methods in visualizing the spectral images, discrimination between different tissue structures is achieved without the use of any stain or fluorescent label. For instance, RGB real color spectral images of the intrinsic emission of mouse skin tissues show blue cells, green hair follicles, and purple collagen fibers. The color signature of each tissue component is directly related to its characteristic emission spectrum. The results of this study show that skin tissue nonlinear intrinsic emission is mainly due to the autofluorescence of reduced nicotinamide adenine dinucleotide (phosphate), flavins, keratin, melanin, phospholipids, elastin and collagen and nonlinear Raman scattering and second-harmonic generation in Type I collagen. In vivo time-lapse spectral imaging is implemented to study metabolic changes in epidermal cells in tissues. Optical scattering in tissues, a key factor in determining the maximum achievable imaging depth, is also investigated in this work.

A COST EFFECTIVE MULTI-SPECTRAL SCANNER FOR NATURAL GAS DETECTION

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yudaya Sivathanu; Jongmook Lim; Vinoo Narayanan

The objective of this project is to design, fabricate and field demonstrate a cost effective, multi-spectral scanner for natural gas leak detection in transmission and distribution pipelines. During the first six months of the project, the design for a laboratory version of the multispectral scanner was completed. The optical, mechanical, and electronic design for the scanner was completed. The optical design was analyzed using Zeemax Optical Design software and found to provide sufficiently resolved performance for the scanner. The electronic design was evaluated using a bread board and very high signal to noise ratios were obtained. Fabrication of a laboratorymore » version of the multi-spectral scanner is currently in progress. A technology status report and a research management plan was also completed during the same period.« less

Combining Direct Broadcast Polar Hyper-spectral Soundings with Geostationary Multi-spectral Imagery for Producing Low Latency Sounding Products

NASA Astrophysics Data System (ADS)

Smith, W.; Weisz, E.; McNabb, J. M. C.

2017-12-01

A technique is described which enables the combination of high vertical resolution (1 to 2-km) JPSS hyper-spectral soundings (i.e., from AIRS, CrIS, and IASI) with high horizontal (2-km) and temporal (15-min) resolution GOES multi-spectral imagery (i.e., provided by ABI) to produce low latency sounding products with the highest possible spatial and temporal resolution afforded by the instruments.

Decorating multi-walled carbon nanotubes with quantum dots for construction of multi-color fluorescent nanoprobes.

PubMed

Jia, Nengqin; Lian, Qiong; Tian, Zhong; Duan, Xin; Yin, Min; Jing, Lihong; Chen, Shouhui; Shen, Hebai; Gao, Mingyuan

2010-01-29

Novel multi-color fluorescent nanoprobes were prepared by electrostatically assembling differently sized CdTe quantum dots on polyethylenimine (PEI) functionalized multi-walled carbon nanotubes (MWNTs). The structural and optical properties of the nano-assemblies (MWNTs-PEI-CdTe) were characterized by transmission electron microscopy (TEM), electron diffraction spectra (EDS), Raman spectroscopy, confocal microscopy and photoluminescence spectroscopy (PL), respectively. Electrochemical impedance spectroscopy (EIS) was also applied to investigate the electrostatic assembling among oxidized MWNTs, PEI and CdTe. Furthermore, confocal fluorescence microscopy was used to monitor the nano-assemblies' delivery into tumor cells. It was found that the nano-assemblies exhibit efficient intracellular transporting and strong intracellular tracking. These properties would make this luminescent nano-assembly an excellent building block for the construction of intracellular nanoprobes, which could hold great promise for biomedical applications.

Hidden Charge States in Soft-X-Ray Laser-Produced Nanoplasmas Revealed by Fluorescence Spectroscopy

NASA Astrophysics Data System (ADS)

Schroedter, L.; Müller, M.; Kickermann, A.; Przystawik, A.; Toleikis, S.; Adolph, M.; Flückiger, L.; Gorkhover, T.; Nösel, L.; Krikunova, M.; Oelze, T.; Ovcharenko, Y.; Rupp, D.; Sauppe, M.; Wolter, D.; Schorb, S.; Bostedt, C.; Möller, T.; Laarmann, T.

2014-05-01

Highly charged ions are formed in the center of composite clusters by strong free-electron laser pulses and they emit fluorescence on a femtosecond time scale before competing recombination leads to neutralization of the nanoplasma core. In contrast to mass spectrometry that detects remnants of the interaction, fluorescence in the extreme ultraviolet spectral range provides fingerprints of transient states of high energy density matter. Spectra from clusters consisting of a xenon core and a surrounding argon shell show that a small fraction of the fluorescence signal comes from multiply charged xenon ions in the cluster core. Initially, these ions are as highly charged as the ions in the outer shells of pure xenon clusters with charge states up to at least 11+.

IRIS Ultraviolet Spectral Properties of a Sample of X-Class Solar Flares

NASA Astrophysics Data System (ADS)

Butler, Elizabeth; Kowalski, Adam; Cauzzi, Gianna; Allred, Joel C.; Daw, Adrian N.

2018-06-01

The white-light (near-ultraviolet (NUV) and optical) continuum emission comprises the majority of the radiated energy in solar flares. However, there are nearly as many explanations for the origin of the white-light continuum radiation as there are white-light flares that have been studied in detail with spectra. Furthermore, there are rarely robust constraints on the time-resolved dynamics in the white-light emitting flare layers. We are conducting a statistical study of the properties of Fe II lines, Mg II lines, and NUV continuum intensity in bright flare kernels observed by the Interface Region Imaging Spectrograph (IRIS), in order to provide comprehensive constraints for radiative-hydrodynamic flare models. Here we present a new technique for identifying bright flare kernels and preliminary relationships among IRIS spectral properties for a sample of X-class solar flares.

Multi-tissue partial volume quantification in multi-contrast MRI using an optimised spectral unmixing approach.

PubMed

Collewet, Guylaine; Moussaoui, Saïd; Deligny, Cécile; Lucas, Tiphaine; Idier, Jérôme

2018-06-01

Multi-tissue partial volume estimation in MRI images is investigated with a viewpoint related to spectral unmixing as used in hyperspectral imaging. The main contribution of this paper is twofold. It firstly proposes a theoretical analysis of the statistical optimality conditions of the proportion estimation problem, which in the context of multi-contrast MRI data acquisition allows to appropriately set the imaging sequence parameters. Secondly, an efficient proportion quantification algorithm based on the minimisation of a penalised least-square criterion incorporating a regularity constraint on the spatial distribution of the proportions is proposed. Furthermore, the resulting developments are discussed using empirical simulations. The practical usefulness of the spectral unmixing approach for partial volume quantification in MRI is illustrated through an application to food analysis on the proving of a Danish pastry. Copyright © 2018 Elsevier Inc. All rights reserved.

The spectral properties of (-)-epigallocatechin 3-O-gallate (EGCG) fluorescence in different solvents: dependence on solvent polarity.

PubMed

Snitsarev, Vladislav; Young, Michael N; Miller, Ross M S; Rotella, David P

2013-01-01

(-)-Epigallocatechin 3-O-gallate (EGCG) a molecule found in green tea and known for a plethora of bioactive properties is an inhibitor of heat shock protein 90 (HSP90), a protein of interest as a target for cancer and neuroprotection. Determination of the spectral properties of EGCG fluorescence in environments similar to those of binding sites found in proteins provides an important tool to directly study protein-EGCG interactions. The goal of this study is to examine the spectral properties of EGCG fluorescence in an aqueous buffer (AB) at pH=7.0, acetonitrile (AN) (a polar aprotic solvent), dimethylsulfoxide (DMSO) (a polar aprotic solvent), and ethanol (EtOH) (a polar protic solvent). We demonstrate that EGCG is a highly fluorescent molecule when excited at approximately 275 nm with emission maxima between 350 and 400 nm depending on solvent. Another smaller excitation peak was found when EGCG is excited at approximately 235 nm with maximum emission between 340 and 400 nm. We found that the fluorescence intensity (FI) of EGCG in AB at pH=7.0 is significantly quenched, and that it is about 85 times higher in an aprotic solvent DMSO. The Stokes shifts of EGCG fluorescence were determined by solvent polarity. In addition, while the emission maxima of EGCG fluorescence in AB, DMSO, and EtOH follow the Lippert-Mataga equation, its fluorescence in AN points to non-specific solvent effects on EGCG fluorescence. We conclude that significant solvent-dependent changes in both fluorescence intensity and fluorescence emission shifts can be effectively used to distinguish EGCG in aqueous solutions from EGCG in environments of different polarity, and, thus, can be used to study specific EGCG binding to protein binding sites where the environment is often different from aqueous in terms of polarity.

Improvement of LOD in Fluorescence Detection with Spectrally Nonuniform Background by Optimization of Emission Filtering.

PubMed

Galievsky, Victor A; Stasheuski, Alexander S; Krylov, Sergey N

2017-10-17

The limit-of-detection (LOD) in analytical instruments with fluorescence detection can be improved by reducing noise of optical background. Efficiently reducing optical background noise in systems with spectrally nonuniform background requires complex optimization of an emission filter-the main element of spectral filtration. Here, we introduce a filter-optimization method, which utilizes an expression for the signal-to-noise ratio (SNR) as a function of (i) all noise components (dark, shot, and flicker), (ii) emission spectrum of the analyte, (iii) emission spectrum of the optical background, and (iv) transmittance spectrum of the emission filter. In essence, the noise components and the emission spectra are determined experimentally and substituted into the expression. This leaves a single variable-the transmittance spectrum of the filter-which is optimized numerically by maximizing SNR. Maximizing SNR provides an accurate way of filter optimization, while a previously used approach based on maximizing a signal-to-background ratio (SBR) is the approximation that can lead to much poorer LOD specifically in detection of fluorescently labeled biomolecules. The proposed filter-optimization method will be an indispensable tool for developing new and improving existing fluorescence-detection systems aiming at ultimately low LOD.

Fluorescence Spectral Properties of All4261 Binding with Phycocyanobilin in E.Coli

NASA Astrophysics Data System (ADS)

Ma, Q.; Zheng, X. J.; Zhou, Z.; Zhou, N.; Zhao, K. H.; Zhou, M.

2014-07-01

Cyanobacteriochromes (CBCRs) are chromophorylated proteins that acting as sensory photoreceptors in cyanobacteria. Based on the bioinformatics of All4261 in Nostoc sp. PCC7120, All4261 is a CBCR apoprotein composed of GAF domains in the N-terminal region. Via polymerase chain reaction with specific primers, All4261 was amplified with genome DNA of Nostoc sp. PCC7120 as template and then subcloned into the expression vector pET30(a+). To survey the fluorescence spectral properties, All4261 was coexpressed with the plasmid that catalyzes phycocyanobilin (PCB) biosynthesis, pACYC-ho1-pcyA, in E.coli BL21. Fluorescence emission spectra and excitation spectra showed that chromophorylated cells containing All4261-PCB had a fluorescence emission peak at 645 nm and a fluorescence excitation peak at 550 nm, but no reversible photoconversion. In order to identify the binding site of PCB in All4261, we obtained three variants All4261(C296L), All4261(C328A), and All4261(C339L), via sitedirected mutagenesis. The binding site was identified as C339 based on the lack of PCB binding of All4261(C339L).

Individual bioaerosol particle discrimination by multi-photon excited fluorescence.

PubMed

Kiselev, Denis; Bonacina, Luigi; Wolf, Jean-Pierre

2011-11-21

Femtosecond laser induced multi-photon excited fluorescence (MPEF) from individual airborne particles is tested for the first time for discriminating bioaerosols. The fluorescence spectra, analysed in 32 channels, exhibit a composite character originating from simultaneous two-photon and three-photon excitation at 790 nm. Simulants of bacteria aggregates (clusters of dyed polystyrene microspheres) and different pollen particles (Ragweed, Pecan, Mulberry) are clearly discriminated by their MPEF spectra. This demonstration experiment opens the way to more sophisticated spectroscopic schemes like pump-probe and coherent control. © 2011 Optical Society of America

Ultraviolet Spectroscopy of Asteroid(4) Vesta

NASA Technical Reports Server (NTRS)

Li, Jian-Yang; Bodewits, Dennis; Feaga, Lori M.; Landsman, Wayne; A'Hearn, Michael F.; Mutchler, Max J.; Russell, Christopher T.; McFadden, Lucy A.; Raymond, Carol A.

2011-01-01

We report a comprehensive review of the UV-visible spectrum and rotational lightcurve of Vesta combining new observations by Hubble Space Telescope and Swift with archival International Ultraviolet Explorer observations. The geometric albedos of Vesta from 220 nm to 953 nm arc derived by carefully comparing these observations from various instruments at different times and observing geometries. Vesta has a rotationally averaged geometric albedo of 0.09 at 250 nm, 0.14 at 300 nm, 0.26 at 373 nm, 0.38 at 673 nm, and 0.30 at 950 nm. The linear spectral slope in the ultraviolet displays a sharp minimum ncar sub-Earth longitude of 20deg, and maximum in the eastern hemisphere. This is completely consistent with the distribution of the spectral slope in the visible wavelength. The uncertainty of the measurement in the ultraviolet is approx.20%, and in the visible wavelengths better than 10%. The amplitude of Vesta's rotational lightcurves is approx.10% throughout the range of wavelengths we observed, but is smaller at 950 nm (approx.6%) ncar the 1-micron mafic band center. Contrary to earlier reports, we found no evidence for any difference between the phasing of the ultraviolet and visible/ncar-infrared lightcurves with respect to sub-Earth longitude. Vesta's average spectrum between 220 and 950 nm can well be described by measured reflectance spectra of fine particle howardite-like materials of basaltic achondrite meteorites. Combining this with the in-phase behavior of the ultraviolet, visible. and ncar-infrared lightcurves, and the spectral slopes with respect to the rotational phase, we conclude that there is no global ultraviolet/visible reversal on Vesta. Consequently, this implies lack of global space weathering on Vesta. Keyword,: Asteroid Vesta; Spectrophotometry; Spectroscopy; Ultraviolet observations; Hubble Space Telescope observations

A single-photon fluorescence and multi-photon spectroscopic study of atherosclerotic lesions

NASA Astrophysics Data System (ADS)

Smith, Michael S. D.; Ko, Alex C. T.; Ridsdale, Andrew; Schattka, Bernie; Pegoraro, Adrian; Hewko, Mark D.; Shiomi, Masashi; Stolow, Albert; Sowa, Michael G.

2009-06-01

In this study we compare the single-photon autofluorescence and multi-photon emission spectra obtained from the luminal surface of healthy segments of artery with segments where there are early atherosclerotic lesions. Arterial tissue was harvested from atherosclerosis-prone WHHL-MI rabbits (Watanabe heritable hyperlipidemic rabbit-myocardial infarction), an animal model which mimics spontaneous myocardial infarction in humans. Single photon fluorescence emission spectra of samples were acquired using a simple spectrofluorometer set-up with 400 nm excitation. Samples were also investigated using a home built multi-photon microscope based on a Ti:sapphire femto-second oscillator. The excitation wavelength was set at 800 nm with a ~100 femto-second pulse width. Epi-multi-photon spectroscopic signals were collected through a fibre-optics coupled spectrometer. While the single-photon fluorescence spectra of atherosclerotic lesions show minimal spectroscopic difference from those of healthy arterial tissue, the multi-photon spectra collected from atherosclerotic lesions show marked changes in the relative intensity of two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) signals when compared with those from healthy arterial tissue. The observed sharp increase of the relative SHG signal intensity in a plaque is in agreement with the known pathology of early lesions which have increased collagen content.

a New Effective way on Vegetation Mornitoring Using Multi-Spectral Canopy LIDAR

NASA Astrophysics Data System (ADS)

Bo, Z.; Wei, G.; Shuo, S.; Shalei, S.; Yingying, M.

2012-07-01

Airborne Laser Scanning (ALS) has been a well-established tool for the measurement of surface topography as well as for the estimation of biophysical canopy variables, such as tree height and vegetation density. By combining GPS and INS together, ALS could acquire surface information effectively in getting the mass production of DEM and DOM. However, up to now most approaches are built upon single-wavelength Lidar system, which could only provide structure information of the vegetation canopy, the intensity information was rarely used to monitor vegetation growing state as its limitation on spectral characteristics. On the other hand, positive multi/hyper-spectral imaging instruments highly rely on the effects of weather, shadow and the background noise etc. The attempts to fuse single-wavelength Lidar data with multi/hyper-spectral data also been effected this way. Thus, a concept for a multi-wavelength, active canopy Lidar has been tested in this paper. The proposed instrument takes measurement at two vegetation-sensitive bands separately at 556 nm and 780 nm, which, according to the correlation analysis between the wavelengths and biochemical content with plenty of ground ASD reflectance dataset, showed a high correlation coefficient on the chlorophyll concentration as well as nitrogen content. The instrumentation of the multi-wavelength canopy Lidar employs low power, solid and semiconductor laser diodes as its laser source and the receiver consists of two channels, one for 556 nm back-scatter signal and the other for 780 nm. The system calibration has also been done by using a standard white board. Multi-wavelength back-scatter signals were collected from a scene consists of stones, healthy broad-leaf trees and unhealthy trees that suffer from disease(part of its leaves were yellow). It is shown that the multi-wavelength canopy Lidar could not only capture the structure information, but also could pick up the spectral characteristics. A further test of three

Broadband cavity enhanced spectroscopy in the ultraviolet spectral region for measurements of nitrogen dioxide and formaldehyde

NASA Astrophysics Data System (ADS)

Washenfelder, R. A.; Attwood, A. R.; Flores, J. M.; Rudich, Y.; Brown, S. S.

2015-09-01

Formaldehyde (CH2O) is the most abundant aldehyde in the atmosphere, and strongly affects photochemistry through its photolysis. We describe simultaneous measurements of CH2O and nitrogen dioxide (NO2) using broadband cavity enhanced spectroscopy in the ultraviolet spectral region. The light source consists of a continuous-wave diode laser focused into a Xenon bulb to produce a plasma that emits high-intensity, broadband light. The plasma discharge is optically filtered and coupled into a 1 m optical cavity. The reflectivity of the cavity mirrors is 0.99933 ± 0.00003 (670 ppm loss) at 338 nm, as determined from the known Rayleigh scattering of He and zero air. This mirror reflectivity corresponds to an effective path length of 1.49 km within the 1 m cell. We measure the cavity output over the 315-350 nm spectral region using a grating monochromator and charge-coupled device (CCD) array detector. We use published reference spectra with spectral fitting software to simultaneously retrieve CH2O and NO2 concentrations. Independent measurements of NO2 standard additions by broadband cavity enhanced spectroscopy and cavity ringdown spectroscopy agree within 2 % (slope for linear fit = 0.98 ± 0.03 with r2 = 0.998). Standard additions of CH2O measured by broadband cavity enhanced spectroscopy and calculated based on flow dilution are also well-correlated, with r2 = 0.9998. During constant, mixed additions of NO2 and CH2O, the 30 s measurement precisions (1σ) of the current configuration were 140 and 210 pptv, respectively. The current 1-min detection limit for extinction measurements at 315-350 nm provides sufficient sensitivity for measurement of trace gases in laboratory experiments and ground-based field experiments. Additionally, the instrument provides highly accurate, spectroscopically-based trace gas detection that may complement higher precision techniques based on non-absolute detection methods. In addition to trace gases, this approach will be appropriate for

Cloud-based processing of multi-spectral imaging data

NASA Astrophysics Data System (ADS)

Bernat, Amir S.; Bolton, Frank J.; Weiser, Reuven; Levitz, David

2017-03-01

Multispectral imaging holds great promise as a non-contact tool for the assessment of tissue composition. Performing multi - spectral imaging on a hand held mobile device would allow to bring this technology and with it knowledge to low resource settings to provide a state of the art classification of tissue health. This modality however produces considerably larger data sets than white light imaging and requires preliminary image analysis for it to be used. The data then needs to be analyzed and logged, while not requiring too much of the system resource or a long computation time and battery use by the end point device. Cloud environments were designed to allow offloading of those problems by allowing end point devices (smartphones) to offload computationally hard tasks. For this end we present a method where the a hand held device based around a smartphone captures a multi - spectral dataset in a movie file format (mp4) and compare it to other image format in size, noise and correctness. We present the cloud configuration used for segmenting images to frames where they can later be used for further analysis.

Detection of Free Thiols and Fluorescence Response of Phycoerythrin Chromophore after Ultraviolet-B Radiation Stress.

PubMed

Kannaujiya, Vinod K; Sinha, Rajeshwar P

2017-03-01

The chemistry of thiol-chromophore linkage plays a central role in the nature of fluorescence of phycoerythrin (PE). Interaction of thiol and chromophore is crucial for the energy transfer, redox signal and inhibition of oxidative damage. In the present investigation the effects of ultraviolet-B radiation on an emission fluorescence intensity and wavelength shift in PE due to interaction between thiol and chromophore by remarkable strategy of detection technique was studied. Purification of PE was done by using a gel permeation and ion exchange chromatography that yielded a quite high purity index (6.40) in a monomeric (αβ) form. UV-B radiation accelerated the quenching efficiency (24.9 ± 1.52%) by reducing fluorescence emission intensity of thiol linked chromophore after 240 min of UV-B exposure. However, after blocking of transiently released free thiol by N-ethylmaleimide, quenching efficiency was increased (36.8 ± 2.80%) with marked emission wavelength shift towards shorter wavelengths up to 562 nm as compared to 575 nm in control. Emission fluorescence of free thiol was at maximum after 240 min that was detected specifically by monobromobimane (mBrB) molecular probe. The association/dissociation of bilin chromophore was analyzed by SDS- and Native-PAGE that also indicated a complete reduction in emission fluorescence. Our work clearly shows an early detection of free thiols and relative interaction with chromophore after UV-B radiation which might play a significant role in structural and functional integrity of terminal PE.

Aluminum nanostructures for ultraviolet plasmonics

NASA Astrophysics Data System (ADS)

Martin, Jérôme; Khlopin, Dmitry; Zhang, Feifei; Schuermans, Silvère; Proust, Julien; Maurer, Thomas; Gérard, Davy; Plain, Jérôme

2017-08-01

An electromagnetic field is able to produce a collective oscillation of free electrons at a metal surface. This allows light to be concentrated in volumes smaller than its wavelength. The resulting waves, called surface plasmons can be applied in various technological applications such as ultra-sensitive sensing, Surface Enhanced Raman Spectroscopy, or metal-enhanced fluorescence, to name a few. For several decades plasmonics has been almost exclusively studied in the visible region by using nanoparticles made of gold or silver as these noble metals support plasmonic resonances in the visible and near-infrared range. Nevertheless, emerging applications will require the extension of nano-plasmonics toward higher energies, in the ultraviolet range. Aluminum is one of the most appealing metal for pushing plasmonics up to ultraviolet energies. The subsequent applications in the field of nano-optics are various. This metal is therefore a highly promising material for commercial applications in the field of ultraviolet nano-optics. As a consequence, aluminum (or ultraviolet, UV) plasmonics has emerged quite recently. Aluminium plasmonics has been demonstrated efficient for numerous potential applications including non-linear optics, enhanced fluorescence, UV-Surface Enhanced Raman Spectroscopy, optoelectronics, plasmonic assisted solid-state lasing, photocatalysis, structural colors and data storage. In this article, different preparation methods developed in the laboratory to obtain aluminum nanostructures with different geometries are presented. Their optical and morphological characterizations of the nanostructures are given and some proof of principle applications such as fluorescence enhancement are discussed.

Wide-field spectrally resolved quantitative fluorescence imaging system: toward neurosurgical guidance in glioma resection

NASA Astrophysics Data System (ADS)

Xie, Yijing; Thom, Maria; Ebner, Michael; Wykes, Victoria; Desjardins, Adrien; Miserocchi, Anna; Ourselin, Sebastien; McEvoy, Andrew W.; Vercauteren, Tom

2017-11-01

In high-grade glioma surgery, tumor resection is often guided by intraoperative fluorescence imaging. 5-aminolevulinic acid-induced protoporphyrin IX (PpIX) provides fluorescent contrast between normal brain tissue and glioma tissue, thus achieving improved tumor delineation and prolonged patient survival compared with conventional white-light-guided resection. However, commercially available fluorescence imaging systems rely solely on visual assessment of fluorescence patterns by the surgeon, which makes the resection more subjective than necessary. We developed a wide-field spectrally resolved fluorescence imaging system utilizing a Generation II scientific CMOS camera and an improved computational model for the precise reconstruction of the PpIX concentration map. In our model, the tissue's optical properties and illumination geometry, which distort the fluorescent emission spectra, are considered. We demonstrate that the CMOS-based system can detect low PpIX concentration at short camera exposure times, while providing high-pixel resolution wide-field images. We show that total variation regularization improves the contrast-to-noise ratio of the reconstructed quantitative concentration map by approximately twofold. Quantitative comparison between the estimated PpIX concentration and tumor histopathology was also investigated to further evaluate the system.

Laser- and Multi-Spectral Monitoring of Natural Objects from UAVs

NASA Astrophysics Data System (ADS)

Reiterer, Alexander; Frey, Simon; Koch, Barbara; Stemmler, Simon; Weinacker, Holger; Hoffmann, Annemarie; Weiler, Markus; Hergarten, Stefan

2016-04-01

The paper describes the research, development and evaluation of a lightweight sensor system for UAVs. The system is composed of three main components: (1) a laser scanning module, (2) a multi-spectral camera system, and (3) a processing/storage unit. All three components are newly developed. Beside measurement precision and frequency, the low weight has been one of the challenging tasks. The current system has a total weight of about 2.5 kg and is designed as a self-contained unit (incl. storage and battery units). The main features of the system are: laser-based multi-echo 3D measurement by a wavelength of 905 nm (totally eye save), measurement range up to 200 m, measurement frequency of 40 kHz, scanning frequency of 16 Hz, relative distance accuracy of 10 mm. The system is equipped with both GNSS and IMU. Alternatively, a multi-visual-odometry system has been integrated to estimate the trajectory of the UAV by image features (based on this system a calculation of 3D-coordinates without GNSS is possible). The integrated multi-spectral camera system is based on conventional CMOS-image-chips equipped with a special sets of band-pass interference filters with a full width half maximum (FWHM) of 50 nm. Good results for calculating the normalized difference vegetation index (NDVI) and the wide dynamic range vegetation index (WDRVI) have been achieved using the band-pass interference filter-set with a FWHM of 50 nm and an exposure times between 5.000 μs and 7.000 μs. The system is currently used for monitoring of natural objects and surfaces, like forest, as well as for geo-risk analysis (landslides). By measuring 3D-geometric and multi-spectral information a reliable monitoring and interpretation of the data-set is possible. The paper gives an overview about the development steps, the system, the evaluation and first results.

Deep multi-spectral ensemble learning for electronic cleansing in dual-energy CT colonography

NASA Astrophysics Data System (ADS)

Tachibana, Rie; Näppi, Janne J.; Hironaka, Toru; Kim, Se Hyung; Yoshida, Hiroyuki

2017-03-01

We developed a novel electronic cleansing (EC) method for dual-energy CT colonography (DE-CTC) based on an ensemble deep convolution neural network (DCNN) and multi-spectral multi-slice image patches. In the method, an ensemble DCNN is used to classify each voxel of a DE-CTC image volume into five classes: luminal air, soft tissue, tagged fecal materials, and partial-volume boundaries between air and tagging and those between soft tissue and tagging. Each DCNN acts as a voxel classifier, where an input image patch centered at the voxel is generated as input to the DCNNs. An image patch has three channels that are mapped from a region-of-interest containing the image plane of the voxel and the two adjacent image planes. Six different types of spectral input image datasets were derived using two dual-energy CT images, two virtual monochromatic images, and two material images. An ensemble DCNN was constructed by use of a meta-classifier that combines the output of multiple DCNNs, each of which was trained with a different type of multi-spectral image patches. The electronically cleansed CTC images were calculated by removal of regions classified as other than soft tissue, followed by a colon surface reconstruction. For pilot evaluation, 359 volumes of interest (VOIs) representing sources of subtraction artifacts observed in current EC schemes were sampled from 30 clinical CTC cases. Preliminary results showed that the ensemble DCNN can yield high accuracy in labeling of the VOIs, indicating that deep learning of multi-spectral EC with multi-slice imaging could accurately remove residual fecal materials from CTC images without generating major EC artifacts.

Terrain Categorization using LIDAR and Multi-Spectral Data

DTIC Science & Technology

2007-01-01

the same spatial resolution cell will be distinguished. 3. PROCESSING The LIDAR data set used in this study was from a discrete-return...smoothing in the spatial dimension. While it was possible to distinguish different classes of materials using this technique, the spatial resolution was...alone and a combination of the two data-types. Results are compared to significant ground truth information. Keywords: LIDAR, multi- spectral

Influence of Luminol Doping of Poly(o-phenylenediamine) on the Spectral, Morphological, and Fluorescent properties: A Potential Fluorescent Marker for Early detection and Diagnosis of Leishmania donovani.

PubMed

Riaz, Ufana; Jadoun, Sapana; Kumar, Prabhat; Arish, Mohd; Rub, Abdur; Ashraf, S M

2017-09-27

There has been a steady progress in the development of doped conjugated polymers to remarkably improve their photo physical properties for their application as biomarkers. With a view to enhance the spectral, morphological, and photo physical properties of poly(o-phenylenediamine) (POPD), the present work reports the synthesis of poly(o-phenylenediamine) and doping of this polymer using luminol. The formation of luminol-doped POPD was confirmed by infrared and ultraviolet-visible spectroscopies and X-ray diffraction studies. The energy band gap values and oscillator strength of luminol in acidic, basic, and neutral media were computed by density functional theory calculations using the B3LYP/6-31G (d) basis set and were compared with experimental data. The luminol doped POPDs show significant in vitro anti-leishmanial activity. Live cell imaging also proved that these molecules bind with the organelle of Leishmania also. These luminol doped POPDs were found non-toxic at the used concentrations on THP-1 derived human macrophage cells through methyl tetrazolium (MTT) assay. The results revealed that luminol doped POPDs were potentially non-toxic to human cells though exhibited immense potential to be used as a fluorescent marker to label Leishmania donovani for diagnostic and other studies.

Laboratory calibration of density-dependent lines in the extreme ultraviolet spectral region

NASA Astrophysics Data System (ADS)

Lepson, J. K.; Beiersdorfer, P.; Gu, M. F.; Desai, P.; Bitter, M.; Roquemore, L.; Reinke, M. L.

2012-05-01

We have been making spectral measurements in the extreme ultraviolet (EUV) from different laboratory sources in order to investigate the electron density dependence of various astrophysically important emission lines and to test the atomic models underlying the diagnostic line ratios. The measurement are being performed at the Livermore EBIT-I electron beam ion trap, the National Spherical Torus Experiment (NSTX) at Princeton, and the Alcator C-Mod tokamak at the Massachusetts Institute of Technology, which together span an electron density of four orders of magnitude and which allow us to test the various models at high and low density limits. Here we present measurements of Fe XXII and Ar XIV, which include new data from an ultra high resolution (λ/Δλ >4000) spectrometer at the EBIT-I facility. We found good agreement between the measurements and modeling calculations for Fe XXII, but poorer agreement for Ar XIV.

Fusion of multi-spectral and panchromatic images based on 2D-PWVD and SSIM

NASA Astrophysics Data System (ADS)

Tan, Dongjie; Liu, Yi; Hou, Ruonan; Xue, Bindang

2016-03-01

A combined method using 2D pseudo Wigner-Ville distribution (2D-PWVD) and structural similarity(SSIM) index is proposed for fusion of low resolution multi-spectral (MS) image and high resolution panchromatic (PAN) image. First, the intensity component of multi-spectral image is extracted with generalized IHS transform. Then, the spectrum diagrams of the intensity components of multi-spectral image and panchromatic image are obtained with 2D-PWVD. Different fusion rules are designed for different frequency information of the spectrum diagrams. SSIM index is used to evaluate the high frequency information of the spectrum diagrams for assigning the weights in the fusion processing adaptively. After the new spectrum diagram is achieved according to the fusion rule, the final fusion image can be obtained by inverse 2D-PWVD and inverse GIHS transform. Experimental results show that, the proposed method can obtain high quality fusion images.

Laser measurement of the spectral extinction coefficients of fluorescent, highly absorbing liquids. [crude petroleum oils

NASA Technical Reports Server (NTRS)

Hoge, F. E.

1982-01-01

A conceptual method is developed to deduce rapidly the spectral extinction coefficient of fluorescent, highly absorbing liquids, such as crude or refined petroleum oils. The technique offers the advantage of only requiring one laser wavelength and a single experimental assembly and execution for any specific fluorescent liquid. The liquid is inserted into an extremely thin wedge-shaped cavity for stimulation by a laser from one side and flurescence measurement on the other side by a monochromator system. For each arbitrarily selected extinction wavelength, the wedge is driven slowly to increasing thicknesses until the fluorescence extinguishes. The fluorescence as a function of wedge thickness permits a determination of the extinction coefficient using an included theoretical model. When the monochromator is set to the laser emission wavelength, the extinction coefficient is determined using the usual on-wavelength signal extinction procedure.

Intrinsic fluorescence excitation-emission matrix spectral features of cottonseed protein fractions and the effects of denaturants

USDA-ARS?s Scientific Manuscript database

To better understand the functional and physicochemical properties of cottonseed protein, we investigated the intrinsic fluorescence excitation-emission matrix (EEM) spectral features of cottonseed protein isolate (CSPI) and sequentially extracted water (CSPw) and alkali (CSPa) protein fractions, an...

Multi-spectral imaging with infrared sensitive organic light emitting diode

PubMed Central

Kim, Do Young; Lai, Tzung-Han; Lee, Jae Woong; Manders, Jesse R.; So, Franky

2014-01-01

Commercially available near-infrared (IR) imagers are fabricated by integrating expensive epitaxial grown III-V compound semiconductor sensors with Si-based readout integrated circuits (ROIC) by indium bump bonding which significantly increases the fabrication costs of these image sensors. Furthermore, these typical III-V compound semiconductors are not sensitive to the visible region and thus cannot be used for multi-spectral (visible to near-IR) sensing. Here, a low cost infrared (IR) imaging camera is demonstrated with a commercially available digital single-lens reflex (DSLR) camera and an IR sensitive organic light emitting diode (IR-OLED). With an IR-OLED, IR images at a wavelength of 1.2 µm are directly converted to visible images which are then recorded in a Si-CMOS DSLR camera. This multi-spectral imaging system is capable of capturing images at wavelengths in the near-infrared as well as visible regions. PMID:25091589

Multi-spectral imaging with infrared sensitive organic light emitting diode

NASA Astrophysics Data System (ADS)

Kim, Do Young; Lai, Tzung-Han; Lee, Jae Woong; Manders, Jesse R.; So, Franky

2014-08-01

Commercially available near-infrared (IR) imagers are fabricated by integrating expensive epitaxial grown III-V compound semiconductor sensors with Si-based readout integrated circuits (ROIC) by indium bump bonding which significantly increases the fabrication costs of these image sensors. Furthermore, these typical III-V compound semiconductors are not sensitive to the visible region and thus cannot be used for multi-spectral (visible to near-IR) sensing. Here, a low cost infrared (IR) imaging camera is demonstrated with a commercially available digital single-lens reflex (DSLR) camera and an IR sensitive organic light emitting diode (IR-OLED). With an IR-OLED, IR images at a wavelength of 1.2 µm are directly converted to visible images which are then recorded in a Si-CMOS DSLR camera. This multi-spectral imaging system is capable of capturing images at wavelengths in the near-infrared as well as visible regions.

Filter Enhances Fluorescent-Penetrant-Inspecting Borescope

NASA Technical Reports Server (NTRS)

Molina, Orlando G.

1990-01-01

Slip-on eyepiece for commercial ultraviolet-light borescope reduces both amount of short-wave ultraviolet light that reaches viewer's eye and apparent intensity of unwanted reflections of white light from surfaces undergoing inspection. Fits on stock eyepiece of borescope, which illuminates surface inspected with intense ultraviolet light. Surface, which is treated with fluorescent dye, emits bright-green visible light wherever dye penetrates - in cracks and voids. Eyepiece contains deep-yellow Wratten 15 (G) filter, which attenuates unwanted light strongly but passes yellow-green fluorescence so defects seen clearly.

A study on the interaction of horse heart cytochrome c with some conventional and ionic liquid surfactants probed by ultraviolet-visible and fluorescence spectroscopic techniques.

PubMed

Mondal, Satyajit; Das, Bijan

2018-06-05

The interactions of a protein cytochrome c with some selected conventional and ionic liquid surfactants have been investigated at pH7.4 using ultraviolet-visible and fluorescence spectroscopic techniques. We used four conventional surfactants - cetyltrimethylammonium bromide (CTAB), dodecyltrimethylammonium bromide (DTAB), sodium N-dodecanoylsarcosinate (SDDS), and N-decanoyl-N-methylglucamine (Mega 10), and a surface active ionic liquid 1-hexadecyl-3-methylimidazolium chloride (C 16 MeImCl). All the investigated surfactants were found to induce an unfolding of the protein cytochrome c. In presence of CTAB, SDDS and C 16 MeImCl, the heme iron atom was found to loose methionine from its axial position. Differential binding of the surfactant monomers and their micelles to the protein molecules was inferred. The ionic surfactants were found to be more effective than the nonionic one in unfolding the investigated protein. However, the extent of binding of CTAB/C 16 MeImCl to cytochrome c reaches a plateau past the critical micellization concentration (cmc) of the surfactant. For each of the cytochrome c-DTAB, cytochrome c-SDDS and cytochrome c-Mega 10 system, although there exists an inflection in the surfactant-binding, saturation point could not be detected. It has been demonstrated from the ultraviolet-visible spectral studies that the oxidation state of iron in cytochrome c does not change when the protein binds with the investigated surfactants. Copyright © 2018 Elsevier B.V. All rights reserved.

Ultraviolet Views of Enceladus, Tethys, and Dione

NASA Technical Reports Server (NTRS)

Hansen, C. J.; Hendrix, A. R.

2005-01-01

The Cassini Ultraviolet Imaging Spectrograph (UVIS) has collected ultraviolet observations of many of Saturn's icy moons since Cassini's insertion into orbit around Saturn. We will report on results from Enceladus, Tethys and Dione, orbiting in the Saturn system at distances of 3.95, 4.88 and 6.26 Saturn radii, respectively. Icy satellite science objectives of the UVIS include investigations of surface age and evolution, surface composition and chemistry, and tenuous exospheres. We address these objectives by producing albedo maps, and reflection and emission spectra, and observing stellar occultations. UVIS has four channels: EUV: Extreme Ultraviolet (55 nm to 110 nm), FUV: Far Ultraviolet (110 to 190 nm), HSP: High Speed Photometer, and HDAC: Hydrogen-Deuterium Absorption Cell. The EUV and FUV spectrographs image onto a 2-dimensional detector, with 64 spatial rows by 1024 spectral columns. To-date we have focused primarily on the far ultraviolet data acquired with the low resolution slit width (4.8 angstrom spectral resolution). Additional information is included in the original extended abstract.

Portable laser synthesizer for high-speed multi-dimensional spectroscopy

DOEpatents

Demos, Stavros G [Livermore, CA; Shverdin, Miroslav Y [Sunnyvale, CA; Shirk, Michael D [Brentwood, CA

2012-05-29

Portable, field-deployable laser synthesizer devices designed for multi-dimensional spectrometry and time-resolved and/or hyperspectral imaging include a coherent light source which simultaneously produces a very broad, energetic, discrete spectrum spanning through or within the ultraviolet, visible, and near infrared wavelengths. The light output is spectrally resolved and each wavelength is delayed with respect to each other. A probe enables light delivery to a target. For multidimensional spectroscopy applications, the probe can collect the resulting emission and deliver this radiation to a time gated spectrometer for temporal and spectral analysis.

Spectrally reconfigurable integrated multi-spot particle trap.

PubMed

Leake, Kaelyn D; Olson, Michael A B; Ozcelik, Damla; Hawkins, Aaron R; Schmidt, Holger

2015-12-01

Optical manipulation of small particles in the form of trapping, pushing, or sorting has developed into a vast field with applications in the life sciences, biophysics, and atomic physics. Recently, there has been increasing effort toward integration of particle manipulation techniques with integrated photonic structures on self-contained optofluidic chips. Here, we use the wavelength dependence of multi-spot pattern formation in multimode interference (MMI) waveguides to create a new type of reconfigurable, integrated optical particle trap. Interfering lateral MMI modes create multiple trapping spots in an intersecting fluidic channel. The number of trapping spots can be dynamically controlled by altering the trapping wavelength. This novel, spectral reconfigurability is utilized to deterministically move single and multiple particles between different trapping locations along the channel. This fully integrated multi-particle trap can form the basis of high throughput biophotonic assays on a chip.

A portable confocal hyperspectral microscope without any scan or tube lens and its application in fluorescence and Raman spectral imaging

NASA Astrophysics Data System (ADS)

Li, Jingwei; Cai, Fuhong; Dong, Yongjiang; Zhu, Zhenfeng; Sun, Xianhe; Zhang, Hequn; He, Sailing

2017-06-01

In this study, a portable confocal hyperspectral microscope is developed. In traditional confocal laser scanning microscopes, scan lens and tube lens are utilized to achieve a conjugate relationship between the galvanometer and the back focal plane of the objective, in order to achieve a better resolution. However, these lenses make it difficult to scale down the volume of the system. In our portable confocal hyperspectral microscope (PCHM), the objective is placed directly next to the galvomirror. Thus, scan lens and tube lens are not included in our system and the size of this system is greatly reduced. Furthermore, the resolution is also acceptable in many biomedical and food-safety applications. Through reducing the optical length of the system, the signal detection efficiency is enhanced. This is conducive to realizing both the fluorescence and Raman hyperspectral imaging. With a multimode fiber as a pinhole, an improved image contrast is also achieved. Fluorescent spectral images for HeLa cells/fingers and Raman spectral images of kumquat pericarp are present. The spectral resolution and spatial resolutions are about 0.4 nm and 2.19 μm, respectively. These results demonstrate that this portable hyperspectral microscope can be used in in-vivo fluorescence imaging and in situ Raman spectral imaging.

Stable multi-domain spectral penalty methods for fractional partial differential equations

NASA Astrophysics Data System (ADS)

Xu, Qinwu; Hesthaven, Jan S.

2014-01-01

We propose stable multi-domain spectral penalty methods suitable for solving fractional partial differential equations with fractional derivatives of any order. First, a high order discretization is proposed to approximate fractional derivatives of any order on any given grids based on orthogonal polynomials. The approximation order is analyzed and verified through numerical examples. Based on the discrete fractional derivative, we introduce stable multi-domain spectral penalty methods for solving fractional advection and diffusion equations. The equations are discretized in each sub-domain separately and the global schemes are obtained by weakly imposed boundary and interface conditions through a penalty term. Stability of the schemes are analyzed and numerical examples based on both uniform and nonuniform grids are considered to highlight the flexibility and high accuracy of the proposed schemes.

Running of scalar spectral index in multi-field inflation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gong, Jinn-Ouk, E-mail: jinn-ouk.gong@apctp.org

We compute the running of the scalar spectral index in general multi-field slow-roll inflation. By incorporating explicit momentum dependence at the moment of horizon crossing, we can find the running straightforwardly. At the same time, we can distinguish the contributions from the quasi de Sitter background and the super-horizon evolution of the field fluctuations.

The source of multi spectral energy of solar energetic electron

DOE Office of Scientific and Technical Information (OSTI.GOV)

Herdiwijaya, Dhani

2015-04-16

We study the solar energetic electron distribution obtained from ACE and GOES satellites which have different altitudes and electron spectral energy during the year 1997 to 2011. The electron spectral energies were 0.038–0.315 MeV from EPAM instrument onboard ACE satellite and >2 MeV from GOES satellite. We found that the low electron energy has no correlation with high energy. In spite of we have corrected to the altitude differences. It implied that they originated from time dependent events with different sources and physical processes at the solar atmosphere. The sources of multi spectral energetic electron were related to flare andmore » CME phenomena. However, we also found that high energetic electron comes from coronal hole.« less

Synthetic Spectral Analysis of the Far Ultraviolet Spectra of the Old Nova HR Del

NASA Astrophysics Data System (ADS)

Robertson, Jordan; Sion, E.

2012-05-01

We present a synthetic spectral analysis of the archival IUE far ultraviolet spectra of the post-nova, HR Del (Nova Del 1967). The system has an estimated white dwarf mass of 0.55 Msun (Ritter and Kolb 2003), orbital period P_orb = 0.214165 days, estimated orbital inclination of 40 degrees (Keurster 1988) and distance determinations in the literature ranging from 970 pc to 285 pc. The spectra reveal P Cygni profiles indicative of wind outflow from the disk and closely resemble the IUE spectra of UX UMa nova-likes, which have never had recorded outbursts. We de-reddened the archival IUE spectra using E(B-V) = 0.16. Our synthetic spectral analysis utilized optically thick, steady state accretion disk models and white dwarf model atmospheres that we constructed using TLUSTY and SYNSPEC (Hubeny 1988, Hubeny and Lanz (1995). Our input parameters were the white dwarf mass, inclination and a range of accretion rates for which we found the best-fitting model. We report the results of our model fitting and compare HR Del with other post-novae at comparable times past their nova outburst. This work was supported by NSF grant 0807892 to Villanova University

Diurnal Variability in Chlorophyll-a, Carotenoids, CDOM and SO₄(2-) Intensity of Offshore Seawater Detected by an Underwater Fluorescence-Raman Spectral System.

PubMed

Chen, Jing; Ye, Wangquan; Guo, Jinjia; Luo, Zhao; Li, Ying

2016-07-13

A newly developed integrated fluorescence-Raman spectral system (λex = 532 nm) for detecting Chlorophyll-a (chl-a), Chromophoric Dissolved Organic Matter (CDOM), carotenoids and SO₄(2-) in situ was used to successfully investigate the diurnal variability of all above. Simultaneously using the integration of fluorescence spectroscopy and Raman spectroscopy techniques provided comprehensive marine information due to the complementarity between the different excitation mechanisms and different selection rules. The investigation took place in offshore seawater of the Yellow Sea (36°05'40'' N, 120°31'32'' E) in October 2014. To detect chl-a, CDOM, carotenoids and SO₄(2-), the fluorescence-Raman spectral system was deployed. It was found that troughs of chl-a and CDOM fluorescence signal intensity were observed during high tides, while the signal intensity showed high values with larger fluctuations during ebb-tide. Chl-a and carotenoids were influenced by solar radiation within a day cycle by different detection techniques, as well as displaying similar and synchronous tendency. CDOM fluorescence cause interference to the measurement of SO₄(2-). To avoid such interference, the backup Raman spectroscopy system with λex = 785 nm was employed to detect SO₄(2-) concentration on the following day. The results demonstrated that the fluorescence-Raman spectral system has great potential in detection of chl-a, carotenoids, CDOM and SO₄(2-) in the ocean.

An instrument for the simultaneous acquisition of size, shape, and spectral fluorescence data from single aerosol particles

NASA Astrophysics Data System (ADS)

Hirst, Edwin; Kaye, Paul H.; Foot, Virginia E.; Clark, James M.; Withers, Philip B.

2004-12-01

We describe the construction of a bio-aerosol monitor designed to capture and record intrinsic fluorescence spectra from individual aerosol particles carried in a sample airflow and to simultaneously capture data relating to the spatial distribution of elastically scattered light from each particle. The spectral fluorescence data recorded by this PFAS (Particle Fluorescence and Shape) monitor contains information relating to the particle material content and specifically to possible biological fluorophores. The spatial scattering data from PFAS yields information relating to particle size and shape. The combination of these data can provide a means of aiding the discrimination of bio-aerosols from background or interferent aerosol particles which may have similar fluorescence properties but exhibit shapes and/or sizes not normally associated with biological particles. The radiation used both to excite particle fluorescence and generate the necessary spatially scattered light flux is provided by a novel compact UV fiber laser operating at 266nm wavelength. Particles drawn from the ambient environment traverse the laser beam in single file. Intrinsic particle fluorescence in the range 300-570nm is collected via an ellipsoidal concentrator into a concave grating spectrometer, the spectral data being recorded using a 16-anode linear array photomultiplier detector. Simultaneously, the spatial radiation pattern scattered by the particle over 5°-30° scattering angle and 360° of azimuth is recorded using a custom designed 31-pixel radial hybrid photodiode array. Data from up to ~5,000 particles per second may be acquired for analysis, usually performed by artificial neural network classification.

Imaging Cellular Dynamics with Spectral Relaxation Imaging Microscopy: Distinct Spectral Dynamics in Golgi Membranes of Living Cells.

PubMed

Lajevardipour, Alireza; Chon, James W M; Chattopadhyay, Amitabha; Clayton, Andrew H A

2016-11-22

Spectral relaxation from fluorescent probes is a useful technique for determining the dynamics of condensed phases. To this end, we have developed a method based on wide-field spectral fluorescence lifetime imaging microscopy to extract spectral relaxation correlation times of fluorescent probes in living cells. We show that measurement of the phase and modulation of fluorescence from two wavelengths permit the identification and determination of excited state lifetimes and spectral relaxation correlation times at a single modulation frequency. For NBD fluorescence in glycerol/water mixtures, the spectral relaxation correlation time determined by our approach exhibited good agreement with published dielectric relaxation measurements. We applied this method to determine the spectral relaxation dynamics in membranes of living cells. Measurements of the Golgi-specific C 6 -NBD-ceramide probe in living HeLa cells revealed sub-nanosecond spectral dynamics in the intracellular Golgi membrane and slower nanosecond spectral dynamics in the extracellular plasma membrane. We interpret the distinct spectral dynamics as a result of structural plasticity of the Golgi membrane relative to more rigid plasma membranes. To the best of our knowledge, these results constitute one of the first measurements of Golgi rotational dynamics.

Broadband cavity-enhanced absorption spectroscopy in the ultraviolet spectral region for measurements of nitrogen dioxide and formaldehyde

NASA Astrophysics Data System (ADS)

Washenfelder, R. A.; Attwood, A. R.; Flores, J. M.; Zarzana, K. J.; Rudich, Y.; Brown, S. S.

2016-01-01

Formaldehyde (CH2O) is the most abundant aldehyde in the atmosphere, and it strongly affects photochemistry through its photolysis. We describe simultaneous measurements of CH2O and nitrogen dioxide (NO2) using broadband cavity-enhanced absorption spectroscopy in the ultraviolet spectral region. The light source consists of a continuous-wave diode laser focused into a Xenon bulb to produce a plasma that emits high-intensity, broadband light. The plasma discharge is optically filtered and coupled into a 1 m optical cavity. The reflectivity of the cavity mirrors is 0.99930 ± 0.00003 (1- reflectivity = 700 ppm loss) at 338 nm, as determined from the known Rayleigh scattering of He and zero air. This mirror reflectivity corresponds to an effective path length of 1.43 km within the 1 m cell. We measure the cavity output over the 315-350 nm spectral region using a grating monochromator and charge-coupled device array detector. We use published reference spectra with spectral fitting software to simultaneously retrieve CH2O and NO2 concentrations. Independent measurements of NO2 standard additions by broadband cavity-enhanced absorption spectroscopy and cavity ring-down spectroscopy agree within 2 % (slope for linear fit = 1.02 ± 0.03 with r2 = 0.998). Standard additions of CH2O measured by broadband cavity-enhanced absorption spectroscopy and calculated based on flow dilution are also well correlated, with r2 = 0.9998. During constant mixed additions of NO2 and CH2O, the 30 s measurement precisions (1σ) of the current configuration were 140 and 210 pptv, respectively. The current 1 min detection limit for extinction measurements at 315-350 nm provides sufficient sensitivity for measurement of trace gases in laboratory experiments and ground-based field experiments. Additionally, the instrument provides highly accurate, spectroscopically based trace gas detection that may complement higher precision techniques based on non