Habitat relationships and nest site characteristics of cavity-nesting birds in cottonwood floodplains

USGS Publications Warehouse

Sedgwick, James A.; Knopf, Fritz L.

1990-01-01

We examined habitat relationships and nest site characteristics for 6 species of cavity-nesting birds--American kestrel (Falco sparverius), northern flicker (Colaptes auratus), red-headed woodpecker (Melanerpes erythrocephalus), black-capped chickadee (Parus atricapillus), house wren (Troglodytes aedon), and European starling (Sturnus vulgaris)--in a mature plains cottonwood (Populus sargentii) bottomland along the South Platte River in northeastern Colorado in 1985 and 1986. We examined characteristics of cavities, nest trees, and the habitat surrounding nest trees. Density of large trees (>69 cm dbh), total length of dead limbs ≥10 cm diameter (TDLL), and cavity density were the most important habitat variables; dead limb length (DLL), dbh, and species were the most important tree variables; and cavity height, cavity entrance diameter, and substrate condition at the cavity (live vs. dead) were the most important cavity variables in segregating cavity nesters along habitat, tree, and cavity dimensions, respectively. Random sites differed most from cavity-nesting bird sites on the basis of dbh, DLL, limb tree density (trees with ≥1 m dead limbs ≥10 cm diameter), and cavity density. Habitats of red-headed woodpeckers and American kestrels were the most unique, differing most from random sites. Based on current trends in cottonwood demography, densities of cavity-nesting birds will probably decline gradually along the South Platte River, paralleling a decline in DLL, limb tree density, snag density, and the concurrent lack of cottonwood regeneration.

In vivo optical characterization of pediatric epileptogenic lesions

NASA Astrophysics Data System (ADS)

Lin, W.-C.; Ragheb, J.; Bhatia, S.; Johnson, Mahlon D.; Sandberg, D.; Fernandez, A.; Morrison, G.; Duchowny, M.; Jayakar, P.

2007-02-01

Epileptogenic lesions and their margins are often difficult to define intraoperatively. We hypothesize that optical spectroscopy can detect unique pathophysiological features of epileptogenic lesions in children and hence differentiate them from normal brain. This hypothesis was tested by comparing the in vivo optical and fluorescence characteristics of epileptogenic brain lesions (non-neoplastic) with those of normal brain. Patients were recruited from those receiving epilepsy surgeries at Miami Children's Hospital. Using a portable spectroscopic system, optical characterization of brain was performed intraoperatively. Fluorescence spectra were measured at 337 nm excitation, and diffuse reflectance spectra were measured between 400 and 850 nm. To date, seven epilepsy patients have been enrolled in the study. A couple interesting trends have been observed in the recorded optical spectra. First, sites within the resection zone, as defined by the intracranial electroencephalogram data, often show higher diffuse reflectance signals than normal sites do. This is especially prominent around 500 nm and between 650 and 850 nm. Secondly, several investigated sites with abnormal electroencephalogram and/or pathology show a unique blue shift in their fluorescence spectra, which is not seen in other cases.

A Universal Isotherm Model to Capture Adsorption Uptake and Energy Distribution of Porous Heterogeneous Surface.

PubMed

Ng, Kim Choon; Burhan, Muhammad; Shahzad, Muhammad Wakil; Ismail, Azahar Bin

2017-09-06

The adsorbate-adsorbent thermodynamics are complex as it is influenced by the pore size distributions, surface heterogeneity and site energy distribution, as well as the adsorbate properties. Together, these parameters defined the adsorbate uptake forming the state diagrams, known as the adsorption isotherms, when the sorption site energy on the pore surfaces are favorable. The available adsorption models for describing the vapor uptake or isotherms, hitherto, are individually defined to correlate to a certain type of isotherm patterns. There is yet a universal approach in developing these isotherm models. In this paper, we demonstrate that the characteristics of all sorption isotherm types can be succinctly unified by a revised Langmuir model when merged with the concepts of Homotattic Patch Approximation (HPA) and the availability of multiple sets of site energy accompanied by their respective fractional probability factors. The total uptake (q/q*) at assorted pressure ratios (P/P s ) are inextricably traced to the manner the site energies are spread, either naturally or engineered by scientists, over and across the heterogeneous surfaces. An insight to the porous heterogeneous surface characteristics, in terms of adsorption site availability has been presented, describing the unique behavior of each isotherm type.

Linking water quality guidelines to the natural characteristics of catchments in order to support distinct aquatic ecosystems: Water quality guidelines for suspended particulate matter

NASA Astrophysics Data System (ADS)

Bilotta, G. S.; Grove, M. K.; Harrison, C.; Joyce, C. B.; Peacock, C.

2012-12-01

The natural characteristics of a catchment provide a template that controls the background rates of geomorphological processes operating within that catchment, which in-turn determines the background physico-chemical and hydro-morphological characteristics of the catchment's surface waters. Large differences in the natural characteristics of catchments (e.g. geology, topography, climate), lead to unique physico-chemical and hydro-morphological conditions that support unique freshwater communities. However, this uniqueness is not always recognised in international water quality guidelines, which often attempt to apply blanket water-quality guidelines to 'protect' a wide range of ecosystems. In this paper we investigate the natural characteristics that control background concentrations of suspended particulate matter (SPM - including nano-scale particles to sand-sized sediments), which is a well-known cause of ecological degradation. At present, the management of SPM is hampered by a lack of understanding of the SPM conditions that water quality managers should aim to achieve in contrasting environments in order to support good ecological status. To address this, in this paper we examine the SPM preferences of contrasting biological communities that are in reference condition (minimal anthropogenic disturbance and high ecological status). We analyse historical SPM data collected on a monthly basis from a wide range of reference-condition temperate environments (638 stream/river sites comprising 42 different biological community-types). This analysis reveals that there are statistically significant differences (One-way ANOVA p < 0.001) between the background SPM concentrations observed in contrasting communities that are in reference condition. Mean background SPM concentrations for contrasting communities ranged from 1.7 to 26.2 mg L-1 (i.e. more than a 15-fold difference). We propose a model for predicting environment-specific water quality guidelines for SPM. In order to develop this model, the 638 reference-condition sites were first classified into one of five mean background SPM ranges (0.00-5.99, 6.00-11.99, 12.00-17.99, 18.00-23.99 and >24.00 mg L-1). Stepwise Multiple Discriminant Analysis (MDA) of these ranges showed that a site's SPM range can be predicted as a function of: mean annual air temperature, mean annual precipitation, mean altitude of upstream catchment, distance from source, slope to source, channel width and depth, the percentage of catchment area comprised of clay, chalk, and hard rock solid geology, and the percentage of the catchment area comprised of blown sand/landslide material as the surface (drift) material. Although the model is still being improved and developed, this research highlights the need to link water quality guidelines to the natural characteristics of catchments and the physico-chemical preferences of the biological communities that would naturally inhabit them.

Landscape genetics of high mountain frog metapopulations

USGS Publications Warehouse

Murphy, M.A.; Dezzani, R.; Pilliod, D.S.; Storfer, A.

2010-01-01

Explaining functional connectivity among occupied habitats is crucial for understanding metapopulation dynamics and species ecology. Landscape genetics has primarily focused on elucidating how ecological features between observations influence gene flow. Functional connectivity, however, may be the result of both these between-site (landscape resistance) landscape characteristics and at-site (patch quality) landscape processes that can be captured using network based models. We test hypotheses of functional connectivity that include both between-site and at-site landscape processes in metapopulations of Columbia spotted frogs (Rana luteiventris) by employing a novel justification of gravity models for landscape genetics (eight microsatellite loci, 37 sites, n = 441). Primarily used in transportation and economic geography, gravity models are a unique approach as flow (e.g. gene flow) is explained as a function of three basic components: distance between sites, production/attraction (e.g. at-site landscape process) and resistance (e.g. between-site landscape process). The study system contains a network of nutrient poor high mountain lakes where we hypothesized a short growing season and complex topography between sites limit R. luteiventris gene flow. In addition, we hypothesized production of offspring is limited by breeding site characteristics such as the introduction of predatory fish and inherent site productivity. We found that R. luteiventris connectivity was negatively correlated with distance between sites, presence of predatory fish (at-site) and topographic complexity (between-site). Conversely, site productivity (as measured by heat load index, at-site) and growing season (as measured by frost-free period between-sites) were positively correlated with gene flow. The negative effect of predation and positive effect of site productivity, in concert with bottleneck tests, support the presence of source-sink dynamics. In conclusion, gravity models provide a powerful new modelling approach for examining a wide range of both basic and applied questions in landscape genetics.

A temporal and spatial assessment of TBT concentrations at dredged material disposal sites around the coast of England and Wales.

PubMed

Bolam, Thi; Barry, Jon; Law, Robin J; James, David; Thomas, Boby; Bolam, Stefan G

2014-02-15

Despite legislative interventions since the 1980s, contemporary concentrations of organotin compounds in marine sediments still impose restrictions on the disposal of dredged material in the UK. Here, we analyse temporal and spatial data to assess the effectiveness of the ban on the use of TBT paints in reducing concentrations at disposal sites. At a national scale, there was a statistically significant increase in the proportion of samples in which the concentration was below the limit of detection (LOD) from 1998 to 2010. This was observed for sediments both inside and outside the disposal sites. However, this temporal decline in organotin concentration is disposal site-specific. Of the four sites studied in detail, two displayed significant increases in proportion of samples below LOD over time. We argue that site-specificity in the effectiveness of the TBT ban results from variations in historical practices at source and unique environmental characteristics of each site. Crown Copyright © 2013. Published by Elsevier Ltd. All rights reserved.

Did You Hear the One About the Doctor? An Examination of Doctor Jokes Posted on Facebook

PubMed Central

Haney, Carol Sue; Weeks, William B; Sirovich, Brenda E; Anthony, Denise L

2014-01-01

Background Social networking sites such as Facebook have become immensely popular in recent years and present a unique opportunity for researchers to eavesdrop on the collective conversation of current societal issues. Objective We sought to explore doctor-related humor by examining doctor jokes posted on Facebook. Methods We performed a cross-sectional study of 33,326 monitored Facebook users, 263 (0.79%) of whom posted a joke that referenced doctors on their Facebook wall during a 6-month observation period (December 15, 2010 to June 16, 2011). We compared characteristics of so-called jokers to nonjokers and identified the characteristics of jokes that predicted joke success measured by having elicited at least one electronic laugh (eg, an LOL or “laughing out loud”) as well as the total number of Facebook “likes” the joke received. Results Jokers told 156 unique doctor jokes and were the same age as nonjokers but had larger social networks (median Facebook friends 227 vs 132, P<.001) and were more likely to be divorced, separated, or widowed (P<.01). In 39.7% (62/156) of unique jokes, the joke was at the expense of doctors. Jokes at the expense of doctors compared to jokes not at the expense of doctors tended to be more successful in eliciting an electronic laugh (46.5% vs 37.3%), although the association was statistically insignificant. In our adjusted models, jokes that were based on current events received considerably more Facebook likes (rate ratio [RR] 2.36, 95% CI 0.97-5.74). Conclusions This study provides insight into the use of social networking sites for research pertaining to health and medicine, including the world of doctor-related humor. PMID:24550095

Did you hear the one about the doctor? An examination of doctor jokes posted on Facebook.

PubMed

Davis, Matthew A; Haney, Carol Sue; Weeks, William B; Sirovich, Brenda E; Anthony, Denise L

2014-02-13

Social networking sites such as Facebook have become immensely popular in recent years and present a unique opportunity for researchers to eavesdrop on the collective conversation of current societal issues. We sought to explore doctor-related humor by examining doctor jokes posted on Facebook. We performed a cross-sectional study of 33,326 monitored Facebook users, 263 (0.79%) of whom posted a joke that referenced doctors on their Facebook wall during a 6-month observation period (December 15, 2010 to June 16, 2011). We compared characteristics of so-called jokers to nonjokers and identified the characteristics of jokes that predicted joke success measured by having elicited at least one electronic laugh (eg, an LOL or "laughing out loud") as well as the total number of Facebook "likes" the joke received. Jokers told 156 unique doctor jokes and were the same age as nonjokers but had larger social networks (median Facebook friends 227 vs 132, P<.001) and were more likely to be divorced, separated, or widowed (P<.01). In 39.7% (62/156) of unique jokes, the joke was at the expense of doctors. Jokes at the expense of doctors compared to jokes not at the expense of doctors tended to be more successful in eliciting an electronic laugh (46.5% vs 37.3%), although the association was statistically insignificant. In our adjusted models, jokes that were based on current events received considerably more Facebook likes (rate ratio [RR] 2.36, 95% CI 0.97-5.74). This study provides insight into the use of social networking sites for research pertaining to health and medicine, including the world of doctor-related humor.

Modeling uranium(VI) adsorption onto montmorillonite under varying carbonate concentrations: A surface complexation model accounting for the spillover effect on surface potential

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tournassat, C.; Tinnacher, R. M.; Grangeon, S.

The prediction of U(VI) adsorption onto montmorillonite clay is confounded by the complexities of: (1) the montmorillonite structure in terms of adsorption sites on basal and edge surfaces, and the complex interactions between the electrical double layers at these surfaces, and (2) U(VI) solution speciation, which can include cationic, anionic and neutral species. Previous U(VI)-montmorillonite adsorption and modeling studies have typically expanded classical surface complexation modeling approaches, initially developed for simple oxides, to include both cation exchange and surface complexation reactions. However, previous models have not taken into account the unique characteristics of electrostatic surface potentials that occur at montmorillonitemore » edge sites, where the electrostatic surface potential of basal plane cation exchange sites influences the surface potential of neighboring edge sites (‘spillover’ effect).« less

Modeling uranium(VI) adsorption onto montmorillonite under varying carbonate concentrations: A surface complexation model accounting for the spillover effect on surface potential

DOE PAGES

Tournassat, C.; Tinnacher, R. M.; Grangeon, S.; ...

2017-10-06

The prediction of U(VI) adsorption onto montmorillonite clay is confounded by the complexities of: (1) the montmorillonite structure in terms of adsorption sites on basal and edge surfaces, and the complex interactions between the electrical double layers at these surfaces, and (2) U(VI) solution speciation, which can include cationic, anionic and neutral species. Previous U(VI)-montmorillonite adsorption and modeling studies have typically expanded classical surface complexation modeling approaches, initially developed for simple oxides, to include both cation exchange and surface complexation reactions. However, previous models have not taken into account the unique characteristics of electrostatic surface potentials that occur at montmorillonitemore » edge sites, where the electrostatic surface potential of basal plane cation exchange sites influences the surface potential of neighboring edge sites (‘spillover’ effect).« less

LLRW disposal facility siting approaches: Connecticut`s innovative volunteer approach

DOE Office of Scientific and Technical Information (OSTI.GOV)

Forcella, D.; Gingerich, R.E.; Holeman, G.R.

1994-12-31

The Connecticut Hazardous Waste Management Service (CHWMS) has embarked on a volunteer approach to siting a LLRW disposal facility in Connecticut. This effort comes after an unsuccessful effort to site a facility using a step-wise, criteria-based site screening process that was a classic example of the decide/announce/defend approach. While some of the specific features of the CHWMS` volunteer process reflect the unique challenge presented by the state`s physical characteristics, political structure and recent unsuccessful siting experience, the basic elements of the process are applicable to siting LLRW disposal facilities in many parts of the United States. The CHWMS` volunteer processmore » is structured to reduce the {open_quotes}outrage{close_quotes} dimension of two of the variables that affect the public`s perception of risk. The two variables are the degree to which the risk is taken on voluntarily (voluntary risks are accepted more readily than those that are imposed) and the amount of control one has over the risk (risks under individual control are accepted more readily than those under government control). In the volunteer process, the CHWMS will only consider sites that have been been voluntarily offered by the community in which they are located and the CHWMS will share control over the development and operation of the facility with the community. In addition to these elements which have broad applicability, the CHWMS has tailored the volunteer approach to take advantage of the unique opportunities made possible by the earlier statewide site screening process. Specifically, the approach presents a {open_quotes}win-win{close_quotes} situation for elected officials in many communities if they decide to participate in the process.« less

Identification of human-to-human transmissibility factors in PB2 proteins of influenza A by large-scale mutual information analysis

PubMed Central

Miotto, Olivo; Heiny, AT; Tan, Tin Wee; August, J Thomas; Brusic, Vladimir

2008-01-01

Background The identification of mutations that confer unique properties to a pathogen, such as host range, is of fundamental importance in the fight against disease. This paper describes a novel method for identifying amino acid sites that distinguish specific sets of protein sequences, by comparative analysis of matched alignments. The use of mutual information to identify distinctive residues responsible for functional variants makes this approach highly suitable for analyzing large sets of sequences. To support mutual information analysis, we developed the AVANA software, which utilizes sequence annotations to select sets for comparison, according to user-specified criteria. The method presented was applied to an analysis of influenza A PB2 protein sequences, with the objective of identifying the components of adaptation to human-to-human transmission, and reconstructing the mutation history of these components. Results We compared over 3,000 PB2 protein sequences of human-transmissible and avian isolates, to produce a catalogue of sites involved in adaptation to human-to-human transmission. This analysis identified 17 characteristic sites, five of which have been present in human-transmissible strains since the 1918 Spanish flu pandemic. Sixteen of these sites are located in functional domains, suggesting they may play functional roles in host-range specificity. The catalogue of characteristic sites was used to derive sequence signatures from historical isolates. These signatures, arranged in chronological order, reveal an evolutionary timeline for the adaptation of the PB2 protein to human hosts. Conclusion By providing the most complete elucidation to date of the functional components participating in PB2 protein adaptation to humans, this study demonstrates that mutual information is a powerful tool for comparative characterization of sequence sets. In addition to confirming previously reported findings, several novel characteristic sites within PB2 are reported. Sequence signatures generated using the characteristic sites catalogue characterize concisely the adaptation characteristics of individual isolates. Evolutionary timelines derived from signatures of early human influenza isolates suggest that characteristic variants emerged rapidly, and remained remarkably stable through subsequent pandemics. In addition, the signatures of human-infecting H5N1 isolates suggest that this avian subtype has low pandemic potential at present, although it presents more human adaptation components than most avian subtypes. PMID:18315849

Selection for avian leukosis virus integration sites determines the clonal progression of B-cell lymphomas

PubMed Central

Malhotra, Sanandan; Justice, James; Morgan, Robin

2017-01-01

Avian leukosis virus (ALV) is a simple retrovirus that causes a wide range of tumors in chickens, the most common of which are B-cell lymphomas. The viral genome integrates into the host genome and uses its strong promoter and enhancer sequences to alter the expression of nearby genes, frequently inducing tumors. In this study, we compare the preferences for ALV integration sites in cultured cells and in tumors, by analysis of over 87,000 unique integration sites. In tissue culture we observed integration was relatively random with slight preferences for genes, transcription start sites and CpG islands. We also observed a preference for integrations in or near expressed and spliced genes. The integration pattern in cultured cells changed over the course of selection for oncogenic characteristics in tumors. In comparison to tissue culture, ALV integrations are more highly selected for proximity to transcription start sites in tumors. There is also a significant selection of ALV integrations away from CpG islands in the highly clonally expanded cells in tumors. Additionally, we utilized a high throughput method to quantify the magnitude of clonality in different stages of tumorigenesis. An ALV-induced tumor carries between 700 and 3000 unique integrations, with an average of 2.3 to 4 copies of proviral DNA per infected cell. We observed increasing tumor clonality during progression of B-cell lymphomas and identified gene players (especially TERT and MYB) and biological processes involved in tumor progression. PMID:29099869

External Barium Affects the Gating of KCNQ1 Potassium Channels and Produces a Pore Block via Two Discrete Sites

PubMed Central

Gibor, Gilad; Yakubovich, Daniel; Peretz, Asher; Attali, Bernard

2004-01-01

The pore properties and the reciprocal interactions between permeant ions and the gating of KCNQ channels are poorly understood. Here we used external barium to investigate the permeation characteristics of homomeric KCNQ1 channels. We assessed the Ba2+ binding kinetics and the concentration and voltage dependence of Ba2+ steady-state block. Our results indicate that extracellular Ba2+ exerts a series of complex effects, including a voltage-dependent pore blockade as well as unique gating alterations. External barium interacts with the permeation pathway of KCNQ1 at two discrete and nonsequential sites. (a) A slow deep Ba2+ site that occludes the channel pore and could be simulated by a model of voltage-dependent block. (b) A fast superficial Ba2+ site that barely contributes to channel block and mostly affects channel gating by shifting rightward the voltage dependence of activation, slowing activation, speeding up deactivation kinetics, and inhibiting channel inactivation. A model of voltage-dependent block cannot predict the complex impact of Ba2+ on channel gating in low external K+ solutions. Ba2+ binding to this superficial site likely modifies the gating transitions states of KCNQ1. Both sites appear to reside in the permeation pathway as high external K+ attenuates Ba2+ inhibition of channel conductance and abolishes its impact on channel gating. Our data suggest that despite the high degree of homology of the pore region among the various K+ channels, KCNQ1 channels display significant structural and functional uniqueness. PMID:15226366

Innate and adaptive immune responses in male and female reproductive tracts in homeostasis and following HIV infection

PubMed Central

Nguyen, Philip V; Kafka, Jessica K; Ferreira, Victor H; Roth, Kristy; Kaushic, Charu

2014-01-01

The male and female reproductive tracts are complex microenvironments that have diverse functional demands. The immune system in the reproductive tract has the demanding task of providing a protective environment for a fetal allograft while simultaneously conferring protection against potential pathogens. As such, it has evolved a unique set of adaptations, primarily under the influence of sex hormones, which make it distinct from other mucosal sites. Here, we discuss the various components of the immune system that are present in both the male and female reproductive tracts, including innate soluble factors and cells and humoral and cell-mediated adaptive immunity under homeostatic conditions. We review the evidence showing unique phenotypic and functional characteristics of immune cells and responses in the male and female reproductive tracts that exhibit compartmentalization from systemic immunity and discuss how these features are influenced by sex hormones. We also examine the interactions among the reproductive tract, sex hormones and immune responses following HIV-1 infection. An improved understanding of the unique characteristics of the male and female reproductive tracts will provide insights into improving clinical treatments of the immunological causes of infertility and the design of prophylactic interventions for the prevention of sexually transmitted infections. PMID:24976268

Feasibility study on cross-linked biopolymeric concrete encapsulating selenium glass wastes.

PubMed

Kim, Daeik; Park, Joon-Seok; Yen, Teh Fu

2012-08-01

Feasibility study was conducted to encapsulate the selenium (Se) contained in glass waste, using the biopolymer-modified concrete. Biopolymer has unique characteristics to provide the chemical sites to metals or toxic compounds through the three-dimensional cross-linked structure. Very minute amount of biopolymer enhanced the characteristics of cementitious material. The resulting biopolymeric composite with selenium glass waste showed 20% higher compressive strength than ordinary concrete and the lower leaching concentration than the equipment detection limit. For a qualitative measurement, X-ray diffraction (XRD; X-ray powder diffractogram) was used to characterize the biopolymeric concrete. The optimum waste content percentage with appropriate biopolymer concrete mixture ratio was identified for its possible commercial use.

Environmental Assessment for Termination of Lease and the Transfer of Property Back to the Landowner for the Morgan City, LA, Tethered Aerostat Radar System (TARS) Site

DTIC Science & Technology

2010-08-01

infrastructure would be affected under Alternative A since all buildings, utility systems, roads, pavements, fences, etc. would be removed. Physical... affect the TARS program objectives. 1.4 ORGANIZATION OF THE DOCUMENT This EA is organized into seven chapters plus appendices. Chapter 1...Action affects public health or safety; • Unique characteristics of the geographic area such as proximity to historic or cultural resources, park

Habitat and movement of lake sturgeon in the upper Mississippi River system, USA

USGS Publications Warehouse

Knights, Brent C.; Vallazza, Jonathon M.; Zigler, Steven J.; Dewey, Michael R.

2002-01-01

Lake sturgeon Acipenser fluvescens, which are now protected from harvest, are considered rare in the upper Mississippi River and little information is available on the remaining populations. Transmitters were implanted into 31 lake sturgeon from two sites in the upper Mississippi River to describe their habitats and movement. The areas surrounding the tagging sites were core areas for both groups of lake sturgeon based on the high use (about 50% of locations by group) and frequent return to these areas by many of the tagged fish. Core areas contained sites with unique hydraulic characteristics, such that depositional substrates were common yet flow was present; these areas probably provide important feeding habitat for lake sturgeon. Minimal geographical overlap in range occurred between groups, suggesting that river reaches and associated core areas were unique to groups or substocks of fish. Lake sturgeon exhibited complex movement behaviors and had ranges of 3-198 km (median, 56 km) during the study. Tagged fish moved both downstream and upstream through upper Mississippi River navigation dams. However, dams appeared to be intermittent barriers to upstream passage because upstream passage events (10 fish, 19 passages) were fewer than downstream events (13 fish, 35 passages). Extensive use of the Wisconsin River by one group of lake sturgeon tagged in the upper Mississippi River has implications regarding management of a threatened population that transcends regulatory boundaries. Our study indicates that lake sturgeon In the upper Mississippi River system share many movement and habitat use characteristics with populations in other systems. However, significant data gaps preclude development of cogent management strategies, including information on population numbers and dynamics, identification of spawning areas, relations between groups, and assessment of the effects of commercial navigation.

Respondent-Driven Sampling in a Multi-Site Study of Black and Latino Men Who Have Sex with Men.

PubMed

Murrill, Christopher S; Bingham, Trista; Lauby, Jennifer; Liu, Kai-Lih; Wheeler, Darrell; Carballo-Diéguez, Alex; Marks, Gary; Millett, Gregorio A

2016-02-01

Respondent-driven sampling (RDS) was used to recruit four samples of Black and Latino men who have sex with men (MSM) in three metropolitan areas to measure HIV prevalence and sexual and drug use behaviors. We compared demographic and behavioral risk characteristics of participants across sites, assessed the extent to which the RDS statistical adjustment procedure provides estimates that differ from the crude results, and summarized our experiences using RDS. From June 2005 to March 2006 a total of 2,235 MSM were recruited and interviewed: 614 Black MSM and 516 Latino MSM in New York City, 540 Black MSM in Philadelphia, and 565 Latino MSM in Los Angeles County. Crude point estimates for demographic characteristics, behavioral risk factors and HIV prevalence were calculated for each of the four samples. RDS Analysis Tool was used to obtain population-based estimates of each sampled population's characteristics. RDS adjusted estimates were similar to the crude estimates for each study sample on demographic characteristics such as age, income, education and employment status. Adjusted estimates of the prevalence of risk behaviors were lower than the crude estimates, and for three of the study samples, the adjusted HIV prevalence estimates were lower than the crude estimates. However, even the adjusted HIV prevalence estimates were higher than what has been previously estimated for these groups of MSM in these cities. Each site faced unique circumstances in implementing RDS. Our experience in using RDS among Black and Latino MSM resulted in diverse recruitment patterns and uncertainties in the estimated HIV prevalence and risk behaviors by study site. Copyright © 2016. Published by Elsevier Inc.

Cloning and polymorphisms of yak lactate dehydrogenase B gene.

PubMed

Wang, Guosheng; Zhao, Xingbo; Zhong, Juming; Cao, Meng; He, Qinghua; Liu, Zhengxin; Lin, Yaqiu; Xu, Yaou; Zheng, Yucai

2013-06-05

The main objective of this work was to study the unique polymorphisms of the lactate dehydrogenase-1 (LDH1) gene in yak (Bos grunniens). Native polyacrylamide gel electrophoresis revealed three phenotypes of LDH1 (a tetramer of H subunit) in yak heart and longissimus muscle extracts. The corresponding gene, ldhb, encoding H subunits of three LDH1 phenotypes was obtained by RT-PCR. A total of six nucleotide differences were detected in yak ldhb compared with that of cattle, of which five mutations cause amino acid substitutions. Sequence analysis shows that the G896A and C689A, mutations of ldhb gene, result in alterations of differently charged amino acids, and create the three phenotypes (F, M, and S) of yak LDH1. Molecular modeling of the H subunit of LDH indicates that the substituted amino acids are not located within NAD+ or substrate binding sites. PCR-RFLP examination of G896A mutation demonstrated that most LDH1-F samples are actually heterozygote at this site. These results help to elucidate the molecular basis and genetic characteristic of the three unique LDH1 phenotypes in yak.

Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene

PubMed Central

Wang, Guosheng; Zhao, Xingbo; Zhong, Juming; Cao, Meng; He, Qinghua; Liu, Zhengxin; Lin, Yaqiu; Xu, Yaou; Zheng, Yucai

2013-01-01

The main objective of this work was to study the unique polymorphisms of the lactate dehydrogenase-1 (LDH1) gene in yak (Bos grunniens). Native polyacrylamide gel electrophoresis revealed three phenotypes of LDH1 (a tetramer of H subunit) in yak heart and longissimus muscle extracts. The corresponding gene, ldhb, encoding H subunits of three LDH1 phenotypes was obtained by RT-PCR. A total of six nucleotide differences were detected in yak ldhb compared with that of cattle, of which five mutations cause amino acid substitutions. Sequence analysis shows that the G896A and C689A, mutations of ldhb gene, result in alterations of differently charged amino acids, and create the three phenotypes (F, M, and S) of yak LDH1. Molecular modeling of the H subunit of LDH indicates that the substituted amino acids are not located within NAD+ or substrate binding sites. PCR-RFLP examination of G896A mutation demonstrated that most LDH1-F samples are actually heterozygote at this site. These results help to elucidate the molecular basis and genetic characteristic of the three unique LDH1 phenotypes in yak. PMID:23739677

Nondestructive detection and measurement of hydrogen embrittlement

DOEpatents

Alex, Franklin; Byrne, Joseph Gerald

1977-01-01

A nondestructive system and method for the determination of the presence and extent of hydrogen embrittlement in metals, alloys, and other crystalline structures subject thereto. Positron annihilation characteristics of the positron-electron annihilation within the tested material provide unique energy distribution curves for each type of material tested at each respective stage of hydrogen embrittlement. Gamma radiation resulting from such annihilation events is detected and statistically summarized by appropriate instrumentation to reveal the variations of electron activity within the tested material caused by hydrogen embrittlement therein. Such data from controlled tests provides a direct indication of the relative stages of hydrogen embrittlement in the form of unique energy distribution curves which may be utilized as calibration curves for future comparison with field tests to give on-site indication of progressive stages of hydrogen embrittlement.

Fundamentals of pulmonary drug delivery.

PubMed

Groneberg, D A; Witt, C; Wagner, U; Chung, K F; Fischer, A

2003-04-01

Aerosol administration of peptide-based drugs plays an important role in the treatment of pulmonary and systemic diseases and the unique cellular properties of airway epithelium offers a great potential to deliver new compounds. As the relative contributions from the large airways to the alveolar space are important to the local and systemic availability, the sites and mechanism of uptake and transport of different target compounds have to be characterized. Among the different respiratory cells, the ciliated epithelial cells of the larger and smaller airways and the type I and type II pneumocytes are the key players in pulmonary drug transport. With their diverse cellular characteristics, each of these cell types displays a unique uptake possibility. Next to the knowledge of these cellular aspects, the nature of aerosolized drugs, characteristics of delivery systems and the depositional and pulmonary clearance mechanisms display major targets to optimize pulmonary drug delivery. Based on the growing knowledge on pulmonary cell biology and pathophysiology due to modern methods of molecular biology, the future characterization of pulmonary drug transport pathways can lead to new strategies in aerosol drug therapy.

Recognizing critical mine spoil health characteristics to design ...

EPA Pesticide Factsheets

Biochar can be used as an amendment to remediate metal-contaminated mine spoils for improved site phytostabilization. For successful phytostabilization to occur, biochar amendments must improve mine spoil health with respect to plant rooting plus uptake of water and nutrients. An inappropriate biochar may negatively impact plant growth conditions resulting in poor plant establishment and growth. Matching the appropriate biochar for each mine site requires reconnaissance of spoil chemical and physical conditions and then identifying which properties need rectified to promote plant growth. A rectification hierarchy needs to be established with the primary limiting factor being addressed first, then successive limitations addressed simultaneously or thereafter. We posit that spoils at each site will have a unique chemical, physical, and biological signature that will affect plant growth. For example, some spoils may be extremely acidic, possess phytotoxic concentrations of heavy metals, or have physical conditions that limits water storage and root penetration. Quantifying these and other conditions beforehand allows for the production of designer biochar with specific characteristics tailored for specific plant growth deficiencies within each spoil. Additionally, we recommend the use of proximally located, undisturbed soils to establish spoil remediation targets. In our work, we have developed a decision-tree flow-chart that identifies salient chemical,

SUMMARY OF TECNIQUES AND UNIQUE USES FOR DIRECT PUSH METHODS IN SITE CHARACTERIZATION ON CONTAMINATED FIELD SITES

EPA Science Inventory

At many of the sites where we have been asked to assist in site characterization, we have discovered severe discrepancies that new technologies may be able to prevent. This presentation is designed to illustrate these new technologies or unique uses of existing technology and the...

Insights into finding a mismatch through the structure of a mispaired DNA bound by a rhodium intercalator

PubMed Central

Pierre, Valérie C.; Kaiser, Jens T.; Barton, Jacqueline K.

2007-01-01

We report the 1.1-Å resolution crystal structure of a bulky rhodium complex bound to two different DNA sites, mismatched and matched in the oligonucleotide 5′-(dCGGAAATTCCCG)2-3′. At the AC mismatch site, the structure reveals ligand insertion from the minor groove with ejection of both mismatched bases and elucidates how destabilized mispairs in DNA may be recognized. This unique binding mode contrasts with major groove intercalation, observed at a matched site, where doubling of the base pair rise accommodates stacking of the intercalator. Mass spectral analysis reveals different photocleavage products associated with the two binding modes in the crystal, with only products characteristic of mismatch binding in solution. This structure, illustrating two clearly distinct binding modes for a molecule with DNA, provides a rationale for the interrogation and detection of mismatches. PMID:17194756

Positive selection sites in tertiary structure of Leguminosae chalcone isomerase 1.

PubMed

Wang, R K; Zhan, S F; Zhao, T J; Zhou, X L; Wang, C E

2015-03-20

Isoflavonoids and the related synthesis enzyme, chalcone isomerase 1 (CHI1), are unique in the Leguminosae, with diverse biological functions. Among the Leguminosae, the soybean is an important oil, protein crop, and model plant. In this study, we aimed to detect the generation pattern of Leguminosae CHI1. Genome-wide sequence analysis of CHI in 3 Leguminosae and 3 other closely related model plants was performed; the expression levels of soybean chalcone isomerases were also analyzed. By comparing positively selected sites and their protein structures, we retrieved the evolution patterns for Leguminosae CHI1. A total of 28 CHI and 7 FAP3 (CHI4) genes were identified and separated into 4 clades: CHI1, CHI2, CHI3, and FAP3. Soybean genes belonging to the same chalcone isomerase subfamily had similar expression patterns. CHI1, the unique chalcone isomerase subfamily in Leguminosae, showed signs of significant positive selection as well as special expression characteristics, indicating an accelerated evolution throughout its divergence. Eight sites were identified as undergoing positive selection with high confidence. When mapped onto the tertiary structure of CHI1, these 8 sites were observed surrounding the enzyme substrate only; some of them connected to the catalytic core of CHI. Thus, we inferred that the generation of Leguminosae CHI1 is dependent on the positively selected amino acids surrounding its catalytic substrate. In other words, the evolution of CHI1 was driven by specific selection or processing conditions within the substrate.

Differential Sox10 Genomic Occupancy in Myelinating Glia

PubMed Central

Lopez-Anido, Camila; Sun, Guannan; Koenning, Matthias; Srinivasan, Rajini; Hung, Holly A.; Emery, Ben; Keles, Sunduz; Svaren, John

2015-01-01

Myelin is formed by specialized myelinating glia: oligodendrocytes and Schwann cells in the central and peripheral nervous systems, respectively. While there are distinct developmental aspects and regulatory pathways in these two cell types, myelination in both systems requires the transcriptional activator Sox10. Sox10 interacts with cell type-specific transcription factors at some loci to induce myelin gene expression, but it is largely unknown how Sox10 transcriptional networks globally compare between oligodendrocytes and Schwann cells. We used in vivo ChIP-Seq analysis of spinal cord and peripheral nerve (sciatic nerve) to identify unique and shared Sox10 binding sites and assess their correlation with active enhancers and transcriptional profiles in oligodendrocytes and Schwann cells. Sox10 binding sites overlap with active enhancers and critical cell type-specific regulators of myelination, such as Olig2 and Myrf in oligodendrocytes, and Egr2/Krox20 in Schwann cells. Sox10 sites also associate with genes critical for myelination in both oligodendrocytes and Schwann cells, and are found within super-enhancers previously defined in brain. In Schwann cells, Sox10 sites contain binding motifs of putative partners in the Sp/Klf, Tead, and nuclear receptor protein families. Specifically, siRNA analysis of nuclear receptors Nr2f1 and Nr2f2 revealed downregulation of myelin genes Mbp and Ndrg1 in primary Schwann cells. Our analysis highlights different mechanisms that establish cell type-specific genomic occupancy of Sox10, which reflects the unique characteristics of oligodendrocyte and Schwann cell differentiation. PMID:25974668

Seafloor massive sulfide deposits support unique megafaunal assemblages: Implications for seabed mining and conservation.

PubMed

Boschen, Rachel E; Rowden, Ashley A; Clark, Malcolm R; Pallentin, Arne; Gardner, Jonathan P A

2016-04-01

Mining of seafloor massive sulfides (SMS) is imminent, but the ecology of assemblages at SMS deposits is poorly known. Proposed conservation strategies include protected areas to preserve biodiversity at risk from mining impacts. Determining site suitability requires biological characterisation of the mine site and protected area(s). Video survey of a proposed mine site and protected area off New Zealand revealed unique megafaunal assemblages at the mine site. Significant relationships were identified between assemblage structure and environmental conditions, including hydrothermal features. Unique assemblages occurred at both active and inactive chimneys and are particularly at risk from mining-related impacts. The occurrence of unique assemblages at the mine site suggests that the proposed protected area is insufficient alone and should instead form part of a network. These results provide support for including hydrothermally active and inactive features within networks of protected areas and emphasise the need for quantitative survey data of proposed sites. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

Identification and Characterization of a Novel Member of the Radical AdoMet Enzyme Superfamily and Implications for the Biosynthesis of the Hmd Hydrogenase Active Site Cofactor▿ †

PubMed Central

McGlynn, Shawn E.; Boyd, Eric S.; Shepard, Eric M.; Lange, Rachel K.; Gerlach, Robin; Broderick, Joan B.; Peters, John W.

2010-01-01

The genetic context, phylogeny, and biochemistry of a gene flanking the H2-forming methylene-H4-methanopterin dehydrogenase gene (hmdA), here designated hmdB, indicate that it is a new member of the radical S-adenosylmethionine enzyme superfamily. In contrast to the characteristic CX3CX2C or CX2CX4C motif defining this family, HmdB contains a unique CX5CX2C motif. PMID:19897660

Unusual inhomogeneous microstructures in charge glass state of PbCrO3

NASA Astrophysics Data System (ADS)

Kurushima, Kosuke; Tsukasaki, Hirofumi; Ogata, Takahiro; Sakai, Yuki; Azuma, Masaki; Ishii, Yui; Mori, Shigeo

2018-05-01

We investigated the microstructures and local structures of perovskite PbCrO3, which shows a metal-to-insulator transition and a 9.8% volume collapse, by electron diffraction, high-resolution transmission electron microscopy (TEM), and high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM). It is revealed that the charge glass state is characterized by the unique coexistence of the crystalline state with a cubic symmetry on average and the noncrystalline state. HAADF-STEM observation at atomic resolution revealed that Pb ions were displaced from the ideal A site position of the cubic perovskite structure, which gives rise to characteristic diffuse scatterings around the fundamental Bragg reflections. These structural inhomogeneities are crucial to the understanding of the unique physical properties in the charge glass state of PbCrO3.

Caspase-2 Is Localized at the Golgi Complex and Cleaves Golgin-160 during Apoptosis

PubMed Central

Mancini, Marie; Machamer, Carolyn E.; Roy, Sophie; Nicholson, Donald W.; Thornberry, Nancy A.; Casciola-Rosen, Livia A.; Rosen, Antony

2000-01-01

Caspases are an extended family of cysteine proteases that play critical roles in apoptosis. Animals deficient in caspases-2 or -3, which share very similar tetrapeptide cleavage specificities, exhibit very different phenotypes, suggesting that the unique features of individual caspases may account for distinct regulation and specialized functions. Recent studies demonstrate that unique apoptotic stimuli are transduced by distinct proteolytic pathways, with multiple components of the proteolytic machinery clustering at distinct subcellular sites. We demonstrate here that, in addition to its nuclear distribution, caspase-2 is localized to the Golgi complex, where it cleaves golgin-160 at a unique site not susceptible to cleavage by other caspases with very similar tetrapeptide specificities. Early cleavage at this site precedes cleavage at distal sites by other caspases. Prevention of cleavage at the unique caspase-2 site delays disintegration of the Golgi complex after delivery of a pro-apoptotic signal. We propose that the Golgi complex, like mitochondria, senses and integrates unique local conditions, and transduces pro-apoptotic signals through local caspases, which regulate local effectors. PMID:10791974

Structural characteristics of alkaline phosphatase from the moderately halophilic bacterium Halomonas sp. 593

DOE Office of Scientific and Technical Information (OSTI.GOV)

Arai, Shigeki; Yonezawa, Yasushi; Ishibashi, Matsujiro

2014-03-01

In order to clarify the structural basis of the halophilic characteristics of an alkaline phosphatase derived from the moderate halophile Halomonas sp. 593 (HaAP), the tertiary structure of HaAP was determined to 2.1 Å resolution by X-ray crystallography. The structural properties of surface negative charge and core hydrophobicity were shown to be intermediate between those characteristic of halophiles and non-halophiles, and may explain the unique functional adaptation to a wide range of salt concentrations. Alkaline phosphatase (AP) from the moderate halophilic bacterium Halomonas sp. 593 (HaAP) catalyzes the hydrolysis of phosphomonoesters over a wide salt-concentration range (1–4 M NaCl). Inmore » order to clarify the structural basis of its halophilic characteristics and its wide-range adaptation to salt concentration, the tertiary structure of HaAP was determined by X-ray crystallography to 2.1 Å resolution. The unit cell of HaAP contained one dimer unit corresponding to the biological unit. The monomer structure of HaAP contains a domain comprised of an 11-stranded β-sheet core with 19 surrounding α-helices similar to those of APs from other species, and a unique ‘crown’ domain containing an extended ‘arm’ structure that participates in formation of a hydrophobic cluster at the entrance to the substrate-binding site. The HaAP structure also displays a unique distribution of negatively charged residues and hydrophobic residues in comparison to other known AP structures. AP from Vibrio sp. G15-21 (VAP; a slight halophile) has the highest similarity in sequence (70.0% identity) and structure (C{sup α} r.m.s.d. of 0.82 Å for the monomer) to HaAP. The surface of the HaAP dimer is substantially more acidic than that of the VAP dimer (144 exposed Asp/Glu residues versus 114, respectively), and thus may enable the solubility of HaAP under high-salt conditions. Conversely, the monomer unit of HaAP formed a substantially larger hydrophobic interior comprising 329 C atoms from completely buried residues, whereas that of VAP comprised 264 C atoms, which may maintain the stability of HaAP under low-salt conditions. These characteristics of HaAP may be responsible for its unique functional adaptation permitting activity over a wide range of salt concentrations.« less

Correlation of Rock Spectra with Quantitative Morphologic Indices: Evidence for a Single Rock Type at the Mars Pathfinder Landing Site

NASA Technical Reports Server (NTRS)

Yingst, R. A.; Biedermann, K. L.; Pierre, N. M.; Haldemann, A. F. C.; Johnson, J. R.

2005-01-01

The Mars Pathfinder (MPF) landing site was predicted to contain a broad sampling of rock types varying in mineralogical, physical, mechanical and geochemical characteristics. Although rocks have been divided into several spectral categories based on Imager for Mars Pathfinder (IMP) visible/near-infrared data, efforts in isolating and classifying spectral units among MPF rocks and soils have met with varying degrees of success, as many factors influencing spectral signatures cannot be quantified to a sufficient level to be removed. It has not been fully determined which spectral categories stem from intrinsic mineralogical differences between rocks or rock surfaces, and which result from factors such as physical or chemical weathering. This has made isolation of unique rock mineralogies difficult. Morphology, like composition, is a characteristic tied to the intrinsic properties and geologic and weathering history of rocks. Rock morphologies can be assessed quantitatively and compared with spectral data, to identify and classify rock types at the MPF landing site. They can also isolate actual rock spectra from spectral types that are surficial in origin, as compositions associated with mantling dust or chemical coatings would presumably not influence rock morphology during weathering events. We previously reported on an initial classification of rocks using the quantitative morphologic indices of size, roundness, sphericity and elongation. Here, we compare this database of rock characteristics with associated rock surface spectra to improve our ability to discriminate between spectra associated with rock types and those from other sources.

Characteristics of patients seeking health information online via social health networks versus general Internet sites: a comparative study.

PubMed

Magnezi, Racheli; Grosberg, Dafna; Novikov, Ilya; Ziv, Arnona; Shani, Mordechai; Freedman, Laurence S

2015-03-01

Camoni.co.il, a Hebrew-language social health network offers advice, consultation, and connection to others with chronic illness. This study compared characteristics and objectives of Camoni.co.il users and individuals seeking medical information through general Internet sites. Similar questionnaires were sent to 1009 Internet and 900 Camoni users. Cluster analysis defined four modes of online social health network use: "acquiring information and support", "communicating", "networking" and "browsing". Six hundred and five Internet and 125 Camoni users responded. Diabetes, hypertension, obesity and lung diseases were found more often among general Internet users than Camoni users. Among Camoni users, "acquiring information and support" was the main motivation for individuals over age 55 years, women, those with lower income, chronic pain, obesity and depression. "Communicating" was the main incentive of men, those 20-34 years old, those with less education, or an eating disorder. "Networking" was the most significant motivation for those with multiple sclerosis or depression. Browsing was most frequent among individuals with multiple sclerosis. Identifying needs of social health network surfers will allow planning unique contents and enhancing social health sites. Physicians might advise patients to use them to obtain support and information regarding their conditions, possibly leading to improved compliance and self-management.

Ceramic-based microelectrode arrays: recording surface characteristics and topographical analysis

PubMed Central

Talauliker, Pooja M.; Price, David A.; Burmeister, Jason J.; Nagari, Silpa; Quintero, Jorge E.; Pomerleau, Francois; Huettl, Peter; Hastings, J. Todd; Gerhardt, Greg A.

2011-01-01

Amperometric measurements using microelectrode arrays (MEAs) provide spatially and temporally resolved measures of neuromolecules in the central nervous system of rats, mice and non-human primates. Multi-site MEAs can be mass fabricated on ceramic (Al2O3) substrate using photolithographic methods, imparting a high level of precision and reproducibility in a rigid but durable recording device. Although the functional capabilities of MEAs have been previously documented for both anesthetized and freely-moving paradigms, the performance enabling intrinsic physical properties of the MEA device have not heretofore been presented. In these studies, spectral analysis confirmed that the MEA recording sites were primarily composed of elemental platinum (Pt°). In keeping with the precision of the photolithographic process, scanning electron microscopy revealed that the Pt recording sites have unique microwell geometries post-fabrication. Atomic force microscopy demonstrated that the recording surfaces have nanoscale irregularities in the form of elevations and depressions, which contribute to increased current per unit area that exceeds previously reported microelectrode designs. The ceramic substrate on the back face of the MEA was characterized by low nanoscale texture and the ceramic sides consisted of an extended network of ridges and cavities. Thus, individual recording sites have a unique Pt° composition and surface profile that has not been previously observed for Pt-based microelectrodes. These features likely impact the physical chemistry of the device, which may influence adhesion of biological molecules and tissue as well as electrochemical recording performance post-implantation. This study is a necessary step towards understanding and extending the performance abilities of MEAs in vivo. PMID:21513736

Voluntary switching between identities in dissociative identity disorder: A functional MRI case study.

PubMed

Savoy, R L; Frederick, B B; Keuroghlian, A S; Wolk, P C

2012-01-01

Patients who suffer from dissociative identity disorder present unique scientific and clinical challenges for psychology and psychiatry. We have been fortunate in working with a patient who-while undergoing functional MRI-can switch rapidly and voluntarily between her main personality (a middle-aged, high-functioning woman) and an alternate personality (a 4-6-year-old girl). A unique task was designed to isolate the processes occurring during the switches between these personalities. Data are from two imaging sessions, conducted months apart, each showing the same activated areas during switches between these personalities. The activated areas include the following: the primary sensory and motor cortex, likely associated with characteristic facial movements made during switching; the nucleus accumbens bilaterally, possibly associated with aspects of reward connected with switching; and prefrontal sites, presumably associated with the executive control involved in the switching of personalities.

Girls with Emotional Disturbance and a History of Arrest: Characteristics and School-Based Predictors of Arrest

ERIC Educational Resources Information Center

Gage, Nicholas A.; Josephs, Nikki L.; Lunde, Kimberly

2012-01-01

Research suggests that girls receiving special education services for Emotional Disturbance (ED) may have unique characteristics and needs. Similarly, juvenile justice research has identified unique characteristics of court-involved girls. This study examined characteristics of girls with ED and a history of arrest. Additionally, classroom-based…

Social Inquiry and Social Action: Priorities for Preparing School Leaders

ERIC Educational Resources Information Center

Gale, Trevor

2010-01-01

Schools are uniquely placed in democracies. Among other things, they are sites of learning about things democratic, including learning "through" as one (important) way of learning "about." There are other sites in which to learn about and through democracies but schooling's uniqueness is that it is a site through which all must pass. So…

Reconstruction of Peripelvic Oncologic Defects.

PubMed

Weichman, Katie E; Matros, Evan; Disa, Joseph J

2017-10-01

After studying this article, the participant should be able to: 1. Understand the anatomy of the peripelvic area. 2. Understand the advantages and disadvantages of performing peripelvic reconstruction in patients undergoing oncologic resection. 3. Select the appropriate local, pedicled, or free-flap reconstruction based on the location of the defect and donor-site characteristics. Peripelvic reconstruction most commonly occurs in the setting of oncologic ablative surgery. The peripelvic area contains several distinct reconstructive regions, including vagina, vulva, penis, and scrotum. Each area provides unique reconstructive considerations. In addition, prior or future radiation therapy or chemotherapy along with cancer cachexia can increase the complexity of reconstruction.

Future perspectives for Antarctic infrared astronomy at Dome C

NASA Astrophysics Data System (ADS)

Ferrari-Toniolo, M.

A brief presentation is done about the objectives, the justifications and the purposes of the PNRA Project entitled: ``A preliminary study of a large Antarctic IR Telescope for Dome C'', with a mention to the up-to-now history and the planned development for the next years. This research does not pretend to be either in competition or in opposition to any of the proposals in progress for IR Astronomical Observations from Antarctica, but wishes to be a contribution toward a full future utilization of the unique site characteristics expected from the High Plateau for IR Astronomy.

Parametric sensitivity analysis of leachate transport simulations at landfills.

PubMed

Bou-Zeid, E; El-Fadel, M

2004-01-01

This paper presents a case study in simulating leachate generation and transport at a 2000 ton/day landfill facility and assesses leachate migration away from the landfill in order to control associated environmental impacts, particularly on groundwater wells down gradient of the site. The site offers unique characteristics in that it is a former quarry converted to a landfill and is planned to have refuse depths that could reach 100 m, making it one of the deepest in the world. Leachate quantity and potential percolation into the subsurface are estimated using the Hydrologic Evaluation of Landfill Performance (HELP) model. A three-dimensional subsurface model (PORFLOW) was adopted to simulate ground water flow and contaminant transport away from the site. A comprehensive sensitivity analysis to leachate transport control parameters was also conducted. Sensitivity analysis suggests that changes in partition coefficient, source strength, aquifer hydraulic conductivity, and dispersivity have the most significant impact on model output indicating that these parameters should be carefully selected when similar modeling studies are performed. Copyright 2004 Elsevier Ltd.

Preparation and high-resolution microscopy of gold cluster labeled nucleic acid conjugates and nanodevices

PubMed Central

Powell, Richard D.; Hainfeld, James F.

2013-01-01

Nanogold and undecagold are covalently linked gold cluster labels which enable the identification and localization of biological components with molecular precision and resolution. They can be prepared with different reactivities, which means they can be conjugated to a wide variety of molecules, including nucleic acids, at specific, unique sites. The location of these sites can be synthetically programmed in order to preserve the binding affinity of the conjugate and impart novel characteristics and useful functionality. Methods for the conjugation of undecagold and Nanogold to DNA and RNA are discussed, and applications of labeled conjugates to the high-resolution microscopic identification of binding sites and characterization of biological macromolecular assemblies are described. In addition to providing insights into their molecular structure and function, high-resolution microscopic methods also show how Nanogold and undecagold conjugates can be synthetically assembled, or self-assemble, into supramolecular materials to which the gold cluster labels impart useful functionality. PMID:20869258

DOE Office of Scientific and Technical Information (OSTI.GOV)

Taneja, Bhupesh; Patel, Asmita; Slesarev, Alexei

Topoisomerases are involved in controlling and maintaining the topology of DNA and are present in all kingdoms of life. Unlike all other types of topoisomerases, similar type IB enzymes have only been identified in bacteria and eukarya. The only putative type IB topoisomerase in archaea is represented by Methanopyrus kandleri topoisomerase V. Despite several common functional characteristics, topoisomerase V shows no sequence similarity to other members of the same type. The structure of the 61 kDa N-terminal fragment of topoisomerase V reveals no structural similarity to other topoisomerases. Furthermore, the structure of the active site region is different, suggesting nomore » conservation in the cleavage and religation mechanism. Additionally, the active site is buried, indicating the need of a conformational change for activity. The presence of a topoisomerase in archaea with a unique structure suggests the evolution of a separate mechanism to alter DNA.« less

High transmittance of X-rays in the utricular otolith of upside-down swimming catfish, Synodontis nigriventris.

PubMed

Ohnishi, Ken; Yamamoto, Toshihiro; Ogawa, Yoichi; Takahashi, Akihisa; Yamashita, Masayuki; Ohnishi, Takeo

2002-03-01

The upside-down swimming catfish (Synodontis nigriventris) has unique behavior, i.e., it frequently shows a stable upside-down posture during swimming and resting. To examine whether the unique postural control in S. nigriventris results from the characteristics of the vestibular organ, we observed the morphological aspects of the otolith and the orientation of sensory hair cells in the utricle. Soft X-ray densitometry analysis showed that the transmittance of soft X-rays in the otolith of S. nigriventris was higher than that in a closely related species (Synodontis multipunctatus) belonging to Synodontis family, goldfish (Carassius auratus) or miniature catfish (Corydoras paleatus) which shows upside-up swimming. The higher transmittance of soft X-rays suggests that the density of the otolith in S. nigriventris is lower than that in S. multipunctatus, C. auratus or C. paleatus. It is possible that the low density of the otolith may have a relation to the control of the unique upside-down posture of S. nigriventris. The hair cells in S. nigriventris were present at the ventral to ventro-lateral site of the utricular epithelium, forming a single hair cell layer as in the other 3 species of fish. The orientation of the sensory hair cells does not appear to cause the unique postural control.

Universality and correlations in individuals wandering through an online extremist space

NASA Astrophysics Data System (ADS)

Cao, Z.; Zheng, M.; Vorobyeva, Y.; Song, C.; Johnson, N. F.

2018-03-01

The "out of the blue" nature of recent terror attacks and the diversity of apparent motives highlight the importance of understanding the online trajectories that individuals follow prior to developing high levels of extremist support. Here we show that the physics of stochastic walks, with and without temporal correlation, provides a unifying description of these online trajectories. Our unique data set comprising all users of a global social media site reveals universal characteristics in individuals' online lifetimes. Our accompanying theory generates analytical and numerical solutions that describe the characteristics shown by individuals that go on to develop high levels of extremist support and those that do not. Going forward, it is conceivable that a deeper understanding of these temporal and many-body correlations may eventually contribute to the important task of better countering the spread of radical propaganda online.

Use of ecoacoustics to determine biodiversity patterns across ecological gradients.

PubMed

Grant, Paul B C; Samways, Michael J

2016-12-01

The variety of local animal sounds characterizes a landscape. We used ecoacoustics to noninvasively assess the species richness of various biotopes typical of an ecofriendly forest plantation with diverse ecological gradients and both nonnative and indigenous vegetation. The reference area was an adjacent large World Heritage Site protected area (PA). All sites were in a global biodiversity hotspot. Our results showed how taxa segregated into various biotopes. We identified 65 singing species, including birds, frogs, crickets, and katydids. Large, natural, protected grassland sites in the PA had the highest mean acoustic diversity (14.1 species/site). Areas covered in nonnative timber or grass species were devoid of acoustic species. Sites grazed by native and domestic megaherbivores were fairly rich (5.1) in acoustic species but none were unique to this habitat type, where acoustic diversity was greater than in intensively managed grassland sites (0.04). Natural vegetation patches inside the plantation mosaic supported high mean acoustic diversity (indigenous forests 7.6, grasslands 8.0, wetlands 9.1), which increased as plant heterogeneity and patch size increased. Indigenous forest patches within the plantation mosaic contained a highly characteristic acoustic species assemblage, emphasizing their complementary contribution to local biodiversity. Overall, acoustic signals determined spatial biodiversity patterns and can be a useful tool for guiding conservation. © 2016 Society for Conservation Biology.

Modifying the photoelectric behavior of bacteriorhodopsin by site-directed mutagenesis: electrochemical and genetic engineering approaches to molecular devices

NASA Astrophysics Data System (ADS)

Hong, F. T.; Hong, F. H.; Needleman, R. B.; Ni, B.; Chang, M.

1992-07-01

Bacteriorhodopsins (bR's) modified by substitution of the chromophore with synthetic vitamin A analogues or by spontaneous mutation have been reported as successful examples of using biomaterials to construct molecular optoelectronic devices. The operation of these devices depends on desirable optical properties derived from molecular engineering. This report examines the effect of site-directed mutagenesis on the photoelectric behavior of bR thin films with an emphasis on their application to the construction of molecular devices based on their unique photoelectric behavior. We examine the photoelectric signals induced by a microsecond light pulse in thin films which contain reconstituted oriented purple membrane sheets isolated from several mutant strains of Halobacterium halobium. A recently developed expression system is used to synthesize mutant bR's in their natural host, H. halobium. We then use a unique analytical method (tunable voltage clamp method) to investigate the effect of pH on the relaxation of two components of the photoelectric signals, B1 and B2. We found that for the four mutant bR's examined, the pH dependence of the B2 component varies significantly. Our results suggest that genetic engineering approaches can produce mutant bR's with altered photoelectric characteristics that can be exploited in the construction of devices.

Adolescent bariatric surgery program characteristics: the Teen Longitudinal Assessment of Bariatric Surgery (Teen-LABS) study experience.

PubMed

Michalsky, Marc P; Inge, Thomas H; Teich, Steven; Eneli, Ihuoma; Miller, Rosemary; Brandt, Mary L; Helmrath, Michael; Harmon, Carroll M; Zeller, Meg H; Jenkins, Todd M; Courcoulas, Anita; Buncher, Ralph C

2014-02-01

The number of adolescents undergoing weight loss surgery (WLS) has increased in response to the increasing prevalence of severe childhood obesity. Adolescents undergoing WLS require unique support, which may differ from adult programs. The aim of this study was to describe institutional and programmatic characteristics of centers participating in Teen Longitudinal Assessment of Bariatric Surgery (Teen-LABS), a prospective study investigating safety and efficacy of adolescent WLS. Data were obtained from the Teen-LABS database, and site survey completed by Teen-LABS investigators. The survey queried (1) institutional characteristics, (2) multidisciplinary team composition, (3) clinical program characteristics, and (4) clinical research infrastructure. All centers had extensive multidisciplinary involvement in the assessment, pre-operative education, and post-operative management of adolescents undergoing WLS. Eligibility criteria and pre-operative clinical and diagnostic evaluations were similar between programs. All programs have well-developed clinical research infrastructure, use adolescent-specific educational resources, and maintain specialty equipment, including high weight capacity diagnostic imaging equipment. The composition of clinical team and institutional resources is consistent with current clinical practice guidelines. These characteristics, coupled with dedicated research staff, have facilitated enrollment of 242 participants into Teen-LABS. © 2013 Published by Elsevier Inc.

Adolescent Bariatric Surgery Program Characteristics: The Teen Longitudinal Assessment of Bariatric Surgery (Teen-LABS) Study Experience

PubMed Central

Michalsky, M.P.; Inge, T.H.; Teich, S.; Eneli, I.; Miller, R.; Brandt, M.L.; Helmrath, M.; Harmon, C.M.; Zeller, M.H.; Jenkins, T.M.; Courcoulas, A.; Buncher, C.R.

2013-01-01

Background The number of adolescents undergoing weight loss surgery (WLS) has increased in response to the increasing prevalence of severe childhood obesity. Adolescents undergoing WLS require unique support, which may differ from adult programs. The aim of this study was to describe institutional and programmatic characteristics of centers participating in Teen-Longitudinal Assessment of Bariatric Surgery (Teen-LABS), a prospective study investigating safety and efficacy of adolescent WLS. Methods Data were obtained from the Teen-LABS database and site survey completed by Teen-LABS investigators. The survey queried (1) institutional characteristics, (2) multidisciplinary team composition, (3) clinical program characteristics, and (4) clinical research infrastructure. Results All centers had extensive multidisciplinary involvement in the assessment, preoperative education and post-operative management of adolescents undergoing WLS. Eligibility criteria, pre-operative clinical and diagnostic evaluations were similar between programs. All programs have well developed clinical research infrastructure, use adolescent-specific educational resources, and maintain specialty equipment, including high weight capacity diagnostic imaging equipment. Conclusions The composition of clinical team and institutional resources are consistent with current clinical practice guidelines. These characteristics, coupled with dedicated research staff, have facilitated enrollment of 242 participants into Teen-LABS. PMID:24491361

Establishing the Antarctic Dome C community reference standard site towards consistent measurements from Earth observation satellites

USGS Publications Warehouse

Cao, C.; Uprety, S.; Xiong, J.; Wu, A.; Jing, P.; Smith, D.; Chander, G.; Fox, N.; Ungar, S.

2010-01-01

Establishing satellite measurement consistency by using common desert sites has become increasingly more important not only for climate change detection but also for quantitative retrievals of geophysical variables in satellite applications. Using the Antarctic Dome C site (75°06′S, 123°21′E, elevation 3.2 km) for satellite radiometric calibration and validation (Cal/Val) is of great interest owing to its unique location and characteristics. The site surface is covered with uniformly distributed permanent snow, and the atmospheric effect is small and relatively constant. In this study, the long-term stability and spectral characteristics of this site are evaluated using well-calibrated satellite instruments such as the Moderate Resolution Imaging Spectroradiometer (MODIS) and Sea-viewing Wide Field-of-view Sensor (SeaWiFS). Preliminary results show that despite a few limitations, the site in general is stable in the long term, the bidirectional reflectance distribution function (BRDF) model works well, and the site is most suitable for the Cal/Val of reflective solar bands in the 0.4–1.0 µm range. It was found that for the past decade, the reflectivity change of the site is within 1.35% at 0.64 µm, and interannual variability is within 2%. The site is able to resolve calibration biases between instruments at a level of ~1%. The usefulness of the site is demonstrated by comparing observations from seven satellite instruments involving four space agencies, including OrbView-2–SeaWiFS, Terra–Aqua MODIS, Earth Observing 1 (EO-1) – Hyperion, Meteorological Operational satellite programme (MetOp) – Advanced Very High Resolution Radiometer (AVHRR), Envisat Medium Resolution Imaging Spectrometer (MERIS) – dvanced Along-Track Scanning Radiometer (AATSR), and Landsat 7 Enhanced Thematic Mapper Plus (ETM+). Dome C is a promising candidate site for climate quality calibration of satellite radiometers towards more consistent satellite measurements, as part of the framework for climate change detection and data quality assurance for the Global Earth Observation System of Systems (GEOSS).

Targeting of Repeated Sequences Unique to a Gene Results in Significant Increases in Antisense Oligonucleotide Potency

PubMed Central

Vickers, Timothy A.; Freier, Susan M.; Bui, Huynh-Hoa; Watt, Andrew; Crooke, Stanley T.

2014-01-01

A new strategy for identifying potent RNase H-dependent antisense oligonucleotides (ASOs) is presented. Our analysis of the human transcriptome revealed that a significant proportion of genes contain unique repeated sequences of 16 or more nucleotides in length. Activities of ASOs targeting these repeated sites in several representative genes were compared to those of ASOs targeting unique single sites in the same transcript. Antisense activity at repeated sites was also evaluated in a highly controlled minigene system. Targeting both native and minigene repeat sites resulted in significant increases in potency as compared to targeting of non-repeated sites. The increased potency at these sites is a result of increased frequency of ASO/RNA interactions which, in turn, increases the probability of a productive interaction between the ASO/RNA heteroduplex and human RNase H1 in the cell. These results suggest a new, highly efficient strategy for rapid identification of highly potent ASOs. PMID:25334092

Characteristics and Concepts of Dynamic Hub Proteins in DNA Processing Machinery from Studies of RPA

PubMed Central

Sugitani, Norie; Chazin, Walter J.

2015-01-01

DNA replication, damage response and repair require the coordinated action of multi-domain proteins operating within dynamic multi-protein machines that act upon the DNA substrate. These modular proteins contain flexible linkers of various lengths, which enable changes in the spatial distribution of the globular domains (architecture) that harbor their essential biochemical functions. This mobile architecture is uniquely suited to follow the evolving substrate landscape present over the course of the specific process performed by the multi-protein machinery. A fundamental advance in understanding of protein machinery is the realization of the pervasive role of dynamics. Not only is the machine undergoing dynamic transformations, but the proteins themselves are flexible and constantly adapting to the progression through the steps of the overall process. Within this dynamic context the activity of the constituent proteins must be coordinated, a role typically played by hub proteins. A number of important characteristics of modular proteins and concepts about the operation of dynamic machinery have been discerned. These provide the underlying basis for the action of the machinery that reads DNA, and responds to and repairs DNA damage. Here, we introduce a number of key characteristics and concepts, including the modularity of the proteins, linkage of weak binding sites, direct competition between sites, and allostery, using the well recognized hub protein replication protein A (RPA). PMID:25542993

Tracking Hole Transport in DNA Hairpins Using a Phenylethynylguanine Nucleobase.

PubMed

Brown, Kristen E; Singh, Arunoday P N; Wu, Yi-Lin; Mishra, Ashutosh Kumar; Zhou, Jiawang; Lewis, Frederick D; Young, Ryan M; Wasielewski, Michael R

2017-08-30

The hole transport dynamics of DNA hairpins possessing a stilbene electron acceptor and donor along with a modified guanine (G) nucleobase, specifically 8-(4'-phenylethynyl)deoxyguanosine, or EG, have been investigated. The nearly indistinguishable oxidation potentials of EG and G and unique spectroscopic characteristics of EG +• make it well-suited for directly observing transient hole occupation during charge transport between a stilbene electron donor and acceptor. In contrast to the cation radical G +• , EG +• possesses a strong absorption near 460 nm and has a distinct Raman-active ethynyl stretch. Both spectroscopic characteristics are easily distinguished from those of the stilbene donor/acceptor radical ion chromophores. Employing EG, we observe its role as a shallow hole trap, or as an intermediate hole transport site when a deeper trap state is present. Using a combination of ultrafast absorption and stimulated Raman spectroscopies, the hole-transport dynamics are observed to be similar in systems having EG vs G bases, with small perturbations to the charge transport rates and yields. These results show EG can be deployed at specified locations throughout the sequence to report on hole occupancy, thereby enabling detailed monitoring of the hole transport dynamics with base-site specificity.

A Profile of Indian Health Service Emergency Departments.

PubMed

Bernard, Kenneth; Hasegawa, Kohei; Sullivan, Ashley; Camargo, Carlos

2017-06-01

The Indian Health Service provides health care to eligible American Indians and Alaskan Natives. No published data exist on emergency services offered by this unique health care system. We seek to determine the characteristics and capabilities of Indian Health Service emergency departments (EDs). All Indian Health Service EDs were surveyed about demographics and operational characteristics for 2014 with the National Emergency Department Inventory survey (available at http://www.emnet-nedi.org/). Of the forty eligible sites, there were 34 respondents (85% response rate). Respondents reported a total of 637,523 ED encounters, ranging from 521 to 63,200 visits per site. Overall, 85% (95% confidence interval 70% to 94%) had continuous physician coverage. Of all physicians staffing the ED, a median of 13% (interquartile range 0% to 50%) were board certified or board prepared in emergency medicine. Overall, 50% (95% confidence interval 34% to 66%) of respondents reported that their ED was operating over capacity. Indian Health Service EDs varied widely in visit volume, with many operating over capacity. Most were not staffed by board-certified or -prepared emergency physicians. Most lacked access to specialty consultation and telemedicine capabilities. Copyright © 2016 American College of Emergency Physicians. Published by Elsevier Inc. All rights reserved.

Lunar Skylights and Their Chemical Compositions

NASA Astrophysics Data System (ADS)

Wong, J.; Torres, J.; FitzHoward, S.; Luu, E.; Hua, J.; Irby, R.

2013-12-01

In 2009, the Japanese orbiter, SELenological and Engineering Explorer (SELENE) discovered a skylight on the near side of the moon. Skylights are collapsed ceilings of rilles, thought to be caused by moonquakes, meteoroids, or incomplete formation of these lava tube ceilings. Since then, NASA's Lunar Reconnaissance Orbiter has discovered two more skylights, also located on the near side of the moon. Previous research has shown that the physical characteristics of known rilles, can be used as indicators of the presence of yet undiscovered rille and lava dome locations across the lunar surface. We hypothesize that skylights have a signature chemical composition that is unique, and can be used to predict the location of additional skylights on the surface of the moon. For this study, we compared chemical composition data of the three mare sites containing skylights with the 21 mare sites without skylights. Using the software JMARS for the Moon, we compiled multiple datasets to measure the concentrations of 13 different chemical compounds including calcium, iron oxide, titanium dioxide, and thorium. We then conducted a two-tailed T-test of the data, which generated probability values for the mean differences across all 13 chemical compounds of the maria sites with skylights and the maria sites without skylights. Our results show that there is no statistical difference in chemical composition across all of the maria sites examined. Therefore, we conclude that chemical composition does not predict or indicate potential skylight locations on the moon. Further research on other skylight characteristics, for example depth and surrounding underground lava channels, may shed light on the relationships between mare and skylights locations. Three Skylight Locations Found on Lunar Surface 100m View of Mare Tranquilitatis Skylight

Discussion of impact of relics activation on protection and utilization approaches-take the old summer palace as an example

NASA Astrophysics Data System (ADS)

Xiaoqi, J.

2015-08-01

As the popularization of cultural relics and the rapid development of cultural tourism industry, a large number of cultural relic tourism resources goes into public eyes. Activation of relics has became an important way for tourist to contact and understand culture relics. The way of how to properly interpret the historical sense and cultural uniqueness to the masses of tourists in order to achieve social service functions of relic resources has always been research focal point of site protection and utilization, so nowadays it has important significance to protection and utilization of heritage resources in our country. From the point of activation of relics and based on the analysis of resource characteristic, the paper in depth discuss ways of activation of relics of the Old Summer Palace, in order to provide reference for sustainable development of sites tourism in China.

Site-specific polarizabilities as descriptors of metallic behavior in atomic clusters

NASA Astrophysics Data System (ADS)

Jackson, Koblar; Jellinek, Julius

The electric dipole polarizability of a cluster is a measure of its response to an applied electric field. The site specific polarizability method decomposes the total cluster polarizability into contributions from individual atoms and also allows it to be partitioned into charge transfer and electric dipole contributions. By systematically examining the trends in these quantities for several types of metal atom clusters over a wide range of cluster sizes, we find common characteristics that uniquely link the behavior of the clusters to that of the corresponding bulk metals for clusters as small as 10 atoms. We discuss these trends and compare and contrast them with results for non-metal clusters. This work was supported by the Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences and Biosciences, U.S. Department of Energy under Grant SC0001330 (KAJ) and Contract No. DE-AC02-06CH11357 (JJ).

Integration of genotoxicity and population genetic analyses in kangaroo rats (Dipodomys merriami) exposed to radionuclide contamination at the Nevada Test Site, USA

USGS Publications Warehouse

Theodorakis, Christopher W.; Bickham, John W.; Lamb, Trip; Medica, Philip A.; Lyne, T. Barrett

2001-01-01

We examined effects of radionuclide exposure at two atomic blast sites on kangaroo rats (Dipodomys merriami) at the Nevada Test Site, Nevada, USA, using genotoxicity and population genetic analyses. We assessed chromosome damage by micronucleus and flow cytometric assays and genetic variation by randomly amplified polymorphic DNA (RAPD) and mitochondrial DNA (mtDNA) analyses. The RAPD analysis showed no population structure, but mtDNA exhibited differentiation among and within populations. Genotoxicity effects were not observed when all individuals were analyzed. However, individuals with mtDNA haplotypes unique to the contaminated sites had greater chromosomal damage than contaminated-site individuals with haplotypes shared with reference sites. When interpopulation comparisons used individuals with unique haplotypes, one contaminated site had greater levels of chromosome damage than one or both of the reference sites. We hypothesize that shared-haplotype individuals are potential migrants and that unique-haplotype individuals are potential long-term residents. A parsimony approach was used to estimate the minimum number of migration events necessary to explain the haplotype distributions on a phylogenetic tree. The observed predominance of migration events into the contaminated sites supported our migration hypothesis. We conclude the atomic blast sites are ecological sinks and that immigration masks the genotoxic effects of radiation on the resident populations.

Magnetic suspension actuator concepts and applications

NASA Technical Reports Server (NTRS)

Kroeger, John

1993-01-01

The fundamental aspect which makes magnetic suspension systems possible is the magnetic phenomena by which significant forces can be generated. Each of these force-producing phenomena has unique characteristics and is implementable in a unique fashion, such that each performs the magnetic suspension task differently than the others. A practical overview of the force-producing concepts, their unique characteristics, and their typical methods of application is provided.

Miniature spectrometer and multispectral imager as a potential diagnostic aid in dermatology

NASA Astrophysics Data System (ADS)

Zeng, Haishan; MacAulay, Calum E.; McLean, David I.; Lui, Harvey; Palcic, Branko

1995-04-01

A miniature spectrometer system has been constructed for both reflectance and autofluorescence spectral measurements of skin. The system is based on PC plug-in spectrometer, therefore, it is miniature and easy to operate. The spectrometer has been used clinically to collect spectral data from various skin lesions including skin cancer. To date, 48 patients with a total of 71 diseased skin sites have been measured. Analysis of these preliminary data suggests that unique spectral characteristics exist for certain types of skin lesions, i.e. seborrheic keratosis, psoriasis, etc.. These spectral characteristics will help the differential diagnosis in Dermatology practice. In conjunction with the spectral point measurements, we are building and testing a multispectral imaging system to measure the spatial distribution of skin reflectance and autofluorescence. Preliminary results indicate that a cutaneous squamous cell carcinoma has a weak autofluorescence signal at the edge of the lesion, but a higher autofluorescence signal in the central area.

Clinical analysis of 48 cases of inverse psoriasis: a hospital-based study.

PubMed

Wang, Gang; Li, Chunying; Gao, Tianwen; Liu, Yufeng

2005-01-01

Inverse psoriasis, rare in clinical practice, refers to psoriasis only or mainly occurring at flexural sites, such as the axilla, antecubital fossae, popliteal fossae, and inguinal creases. It is also known as flexural psoriasis. With a total collection of psoriatic cases from September 2002 to December 2003 at Xijing hospital, we made a retrospective analysis of the disease history, clinical characteristics, and treatment of the patients affected with inverse psoriasis. The results showed that the major clinical manifestations of inverse psoriasis were sharply demarcated erythematous plaques with varying degrees of infiltration and minimal or no scales. Affected areas often involve the groin, axilla, genitals, and umbilicus. The disease responds well to the narrow band UVB therapy. Compared with common psoriasis, inverse psoriasis has similar and unique characteristics in terms of the affected areas, clinical symptoms, and responses to the treatment.

Hydrologic data for computation of sediment discharge : Toutle and North Fork Toutle Rivers near Mount St. Helens, Washington, water years 1980-84

USGS Publications Warehouse

Childers, Dallas; Hammond, Stephen E.; Johnson, William P.

1988-01-01

Immediately after the devastating May 18, 1980, eruption of Mount St. Helens, a program was initiated by the U.S. Geological Survey to study the streamflow and sediment characteristics of streams impacted by the eruption. Some of the data gathered in that program are presented in this report. Data are presented for two key sites in the Toutle River basin: North Fork Toutle River near Kid Valley, and Toutle River at Tower Road, near Silver Lake. The types of data presented are appropriate for use with sediment transport formulas; however, the data are also intended for use in a wide variety of additional applications. The data presented in this report are unique because they delineate flow conditions possessing great potential fo sediment transport. The data define unusually high suspended-sediment concentration. Data defining hydraulic, peak discharge, suspended-sediment, and bed-material characteristics are presented. (USGS)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pind, C.

The SECURE heating reactor was designed by ASEA-ATOM as a realistic alternative for district heating in urban areas and for supplying heat to process industries. SECURE has unique safety characteristics, that are based on fundamental laws of physics. The safety does not depend on active components or operator intervention for shutdown and cooling of the reactor. The inherent safety characteristics of the plant cannot be affected by operator errors. Due to its very low environment impact, it can be sited close to heat consumers. The SECURE heating reactor has been shown to be competitive in comparison with other alternatives formore » heating Helsinki and Seoul. The SECURE heating reactor forms a basis for the power-producing SECURE-P reactor known as PIUS (Process Inherent Ultimate Safety), which is based on the same inherent safety principles. The thermohydraulic function and transient response have been demonstrated in a large electrically heated loop at the ASEA-ATOM laboratories.« less

Structural characteristics of alkaline phosphatase from the moderately halophilic bacterium Halomonas sp. 593

PubMed Central

Arai, Shigeki; Yonezawa, Yasushi; Ishibashi, Matsujiro; Matsumoto, Fumiko; Adachi, Motoyasu; Tamada, Taro; Tokunaga, Hiroko; Blaber, Michael; Tokunaga, Masao; Kuroki, Ryota

2014-01-01

Alkaline phosphatase (AP) from the moderate halophilic bacterium Halomonas sp. 593 (HaAP) catalyzes the hydrolysis of phosphomonoesters over a wide salt-concentration range (1–4 M NaCl). In order to clarify the structural basis of its halophilic characteristics and its wide-range adaptation to salt concentration, the tertiary structure of HaAP was determined by X-ray crystallography to 2.1 Å resolution. The unit cell of HaAP contained one dimer unit corresponding to the biological unit. The monomer structure of HaAP contains a domain comprised of an 11-stranded β-sheet core with 19 surrounding α-helices similar to those of APs from other species, and a unique ‘crown’ domain containing an extended ‘arm’ structure that participates in formation of a hydrophobic cluster at the entrance to the substrate-binding site. The HaAP structure also displays a unique distribution of negatively charged residues and hydrophobic residues in comparison to other known AP structures. AP from Vibrio sp. G15-21 (VAP; a slight halophile) has the highest similarity in sequence (70.0% identity) and structure (Cα r.m.s.d. of 0.82 Å for the monomer) to HaAP. The surface of the HaAP dimer is substantially more acidic than that of the VAP dimer (144 exposed Asp/Glu residues versus 114, respectively), and thus may enable the solubility of HaAP under high-salt conditions. Conversely, the monomer unit of HaAP formed a substantially larger hydrophobic interior comprising 329 C atoms from completely buried residues, whereas that of VAP comprised 264 C atoms, which may maintain the stability of HaAP under low-salt conditions. These characteristics of HaAP may be responsible for its unique functional adaptation permitting activity over a wide range of salt concentrations. PMID:24598750

Sensing site-specific structural characteristics and chirality using vibrational circular dichroism of isotope labeled peptides.

PubMed

Keiderling, Timothy A

2017-12-01

Isotope labeling has a long history in chemistry as a tool for probing structure, offering enhanced sensitivity, or enabling site selection with a wide range of spectroscopic tools. Chirality sensitive methods such as electronic circular dichroism are global structural tools and have intrinsically low resolution. Consequently, they are generally insensitive to modifications to enhance site selectivity. The use of isotope labeling to modify vibrational spectra with unique resolvable frequency shifts can provide useful site-specific sensitivity, and these methods have been recently more widely expanded in biopolymer studies. While the spectral shifts resulting from changes in isotopic mass can provide resolution of modes from specific parts of the molecule and can allow detection of local change in structure with perturbation, these shifts alone do not directly indicate structure or chirality. With vibrational circular dichroism (VCD), the shifted bands and their resultant sign patterns can be used to indicate local conformations in labeled biopolymers, particularly if multiple labels are used and if their coupling is theoretically modeled. This mini-review discusses selected examples of the use of labeling specific amides in peptides to develop local structural insight with VCD spectra. © 2017 Wiley Periodicals, Inc.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nichols, Teresa A.; Lapsa, Melissa Voss

Oak Ridge National Laboratory (ORNL) is both the largest science and energy laboratory of the US Department of Energy (DOE) and one of the oldest national laboratories still operating at its original site. These characteristics provide the Sustainable Campus Initiative (SCI) both a unique opportunity and a unique challenge to integrate sustainability into facilities and activities. As outlined in this report, SCI is leveraging the outcomes of ORNL’s DOE-sponsored research and development programs to maximize the efficient use of energy and natural resources across ORNL. Wherever possible, ORNL is integrating technical innovations into new and existing facilities, systems, and processesmore » with a widespread approach to achieving Executive Order 13514. ORNL continues to pursue and deploy innovative solutions and initiatives to advance regional, national, and worldwide sustainability and continues to transform its culture and engage employees in supporting sustainability at work, at home, and in the community. Table 1 summarizes ORNL's FY 2013 performance and planned actions to attain future goals. ORNL has achieved numerous successes during FY 2013, which are described in detail throughout this document.« less

Symbolic emblems of the Levantine Aurignacians as a regional entity identifier (Hayonim Cave, Lower Galilee, Israel).

PubMed

Tejero, José-Miguel; Belfer-Cohen, Anna; Bar-Yosef, Ofer; Gutkin, Vitaly; Rabinovich, Rivka

2018-05-15

The Levantine Aurignacian is a unique phenomenon in the local Upper Paleolithic sequence, showing greater similarity to the West European classic Aurignacian than to the local Levantine archaeological entities preceding and following it. Herewith we highlight another unique characteristic of this entity, namely, the presence of symbolic objects in the form of notched bones (mostly gazelle scapulae) from the Aurignacian levels of Hayonim Cave, Lower Galilee, Israel. Through both macroscopic and microscopic analyses of the items, we suggest that they are not mere cut marks but rather are intentional (decorative?) human-made markings. The significance of this evidence for symbolic behavior is discussed in its chrono-cultural and geographical contexts. Notched bones are among the oldest symbolic expressions of anatomically modern humans. However, unlike other Paleolithic sites where such findings were reported in single numbers, the number of these items recovered at Hayonim Cave is sufficient to assume they possibly served as an emblem of the Levantine Aurignacian.

Comparison of mosquito control programs in seven urban sites in Africa, the Middle East, and the Americas.

PubMed

Impoinvil, Daniel E; Ahmad, Sajjad; Troyo, Adriana; Keating, Joseph; Githeko, Andrew K; Mbogo, Charles M; Kibe, Lydiah; Githure, John I; Gad, Adel M; Hassan, Ali N; Orshan, Laor; Warburg, Alon; Calderón-Arguedas, Olger; Sánchez-Loría, Victoria M; Velit-Suarez, Rosanna; Chadee, Dave D; Novak, Robert J; Beier, John C

2007-10-01

Mosquito control programs at seven urban sites in Kenya, Egypt, Israel, Costa Rica, and Trinidad are described and compared. Site-specific urban and disease characteristics, organizational diagrams, and strengths, weaknesses, obstacles and threats (SWOT) analysis tools are used to provide a descriptive assessment of each mosquito control program, and provide a comparison of the factors affecting mosquito abatement. The information for SWOT analysis is collected from surveys, focus-group discussions, and personal communication. SWOT analysis identified various issues affecting the efficiency and sustainability of mosquito control operations. The main outcome of our work was the description and comparison of mosquito control operations within the context of each study site's biological, social, political, management, and economic conditions. The issues identified in this study ranged from lack of inter-sector collaboration to operational issues of mosquito control efforts. A lack of sustainable funding for mosquito control was a common problem for most sites. Many unique problems were also identified, which included lack of mosquito surveillance, lack of law enforcement, and negative consequences of human behavior. Identifying common virtues and shortcomings of mosquito control operations is useful in identifying "best practices" for mosquito control operations, thus leading to better control of mosquito biting and mosquito-borne disease transmission.

Siting industrial waste land disposal facilities in Thailand: A risk based approach

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fingleton, D.J.; Habegger, L.; Peters, R.

The Thailand Industrial Works Department (IWD) has established a toxic industrial waste Central Treatment and Stabilization Center (CTSC) for textile dyeing and electroplating industries located in the Thonburi region of the Bangkok metropolitan area. Industrial waste is treated, stabilized, and stored at the CTSC. Although the IWD plans to ship the stabilized sludge to the Ratchaburi Province in western Thailand for burial, the location for the land disposal site has not been selected. Assessing the relative health risks from exposure to toxic chemicals released from an industrial waste land disposal site is a complicated, data-intensive process that requires a multidisciplinarymore » approach. This process is further complicated by the unique physical and cultural characteristics exhibited by the rapidly industrializing Thai economy. The purpose of this paper is to describe the research approach taken and to detail the constraints to health risk assessments in Thailand. issues discussed include data availability and quality, effectiveness of control or mitigation methods, cultural differences, and the basic assumptions inherent in many of the risk assessment components.« less

Water column and bed-sediment core samples collected from Brownlee Reservoir near Oxbow, Oregon, 2012

USGS Publications Warehouse

Fosness, Ryan L.; Naymik, Jesse; Hopkins, Candice B.; DeWild, John F.

2013-01-01

The U.S. Geological Survey, in cooperation with Idaho Power Company, collected water-column and bed-sediment core samples from eight sites in Brownlee Reservoir near Oxbow, Oregon, during May 5–7, 2012. Water-column and bed-sediment core samples were collected at each of the eight sites and analyzed for total mercury and methylmercury. Additional bed-sediment core samples, collected from three of the eight sites, were analyzed for pesticides and other organic compounds, trace metals, and physical characteristics, such as particle size. Total mercury and methylmercury were detected in each of the water column and bed-sediment core samples. Only 17 of the 417 unique pesticide and organic compounds were detected in bed-sediment core samples. Concentrations of most organic wastewater compounds detected in bed sediment were less than the reporting level. Trace metals detected were greater than the reporting level in all the bed-sediment core samples submitted for analysis. The particle size distribution of bed-sediment core samples was predominantly clay mixed with silt.

X-ray diffraction and Raman spectroscopy study of white decorations on tricolored ceramics from Northwestern Argentina

NASA Astrophysics Data System (ADS)

Freire, E.; Acevedo, V.; Halac, E. B.; Polla, G.; López, M.; Reinoso, M.

2016-03-01

White virgules, commas, and dot designs on tricolored ceramics are sporadically found in different archaeological sites located in Northwestern Argentina area, as Puna and Quebrada de Humahuaca. This decorating style has been reported in several articles, but few previous archaeometric studies have been carried out on the pigment composition. Fragments from Puna and Quebrada archaeological sites, belonging to Regional Development Period (900-1430 AD), were analyzed by X-ray diffraction and Raman spectroscopy in order to characterize the pigments employed. Red and black pigments are based on iron and manganese oxides, as it has been extensively reported for the NW Argentina area. White pigments from white virgules, comma, and dot designs have shown different composition. Hydroxyapatite was found in samples from Doncellas site (North Puna region), and calcium and calcium-magnesium containing compounds, as vaterite and dolomite, along with titanium containing compounds were detected on samples from Abralaite (Central Puna region) and Gasoducto (Quebrada de Humahuaca region). It has been concluded that pigment composition is not characteristic of a unique region.

X-ray diffraction and Raman spectroscopy study of white decorations on tricolored ceramics from Northwestern Argentina.

PubMed

Freire, E; Acevedo, V; Halac, E B; Polla, G; López, M; Reinoso, M

2016-03-15

White virgules, commas, and dot designs on tricolored ceramics are sporadically found in different archaeological sites located in Northwestern Argentina area, as Puna and Quebrada de Humahuaca. This decorating style has been reported in several articles, but few previous archaeometric studies have been carried out on the pigment composition. Fragments from Puna and Quebrada archaeological sites, belonging to Regional Development Period (900-1430 AD), were analyzed by X-ray diffraction and Raman spectroscopy in order to characterize the pigments employed. Red and black pigments are based on iron and manganese oxides, as it has been extensively reported for the NW Argentina area. White pigments from white virgules, comma, and dot designs have shown different composition. Hydroxyapatite was found in samples from Doncellas site (North Puna region), and calcium and calcium-magnesium containing compounds, as vaterite and dolomite, along with titanium containing compounds were detected on samples from Abralaite (Central Puna region) and Gasoducto (Quebrada de Humahuaca region). It has been concluded that pigment composition is not characteristic of a unique region. Copyright © 2015 Elsevier B.V. All rights reserved.

Stimulus-responsive zinc oxide-functionalized macromolecular humic acid nanocarrier for enhancement of antibacterial activity of ciprofloxacin hydrochloride.

PubMed

Murugesan, Gowri; Latha, Nachimuthu; Suganya, Kannan; Murugan, Marudhamuthu; Munusamy, Murugan A; Rajan, Mariappan

2018-07-15

Macromolecular of naturally occurring humic acid (HA) have garnered interest in the chemical, biological and medicine industry owing to their unique behavior, i.e., strong adsorptive and non-toxic nature. Here, we investigated the functionalization of organic (HA) with inorganic (ZnO) hybrid nanoparticles for topical and site-targeted delivery of ciprofloxacin by simple emulsification techniques. Ciprofloxacin (CIPRO)-encapsulated hybrid nanocarrier constitute an attractive novel drug delivery vehicle for sustained release of antibiotics to bacterial infection sites in an extended and controlled manner. The analytical characteristics of the designed system were thoroughly investigated by FTIR, XRD, SEM/EDAX, and TEM. The drug release of ciprofloxacin over 24h was 87.5%, 98.03%, 97.44%, and 97.24% for pH2.5, 5.5, 6.8, and 8.0, respectively. The antibacterial activities results confirmed that the CIPRO-encapsulated hybrid nanocarrier showed excellent growth inhibition against microorganisms. This hybrid nanocarrier loaded with antibiotics represents a promising approach for targeted and controlled drug delivery to infected sites. Copyright © 2018 Elsevier B.V. All rights reserved.

Evolution of a mass spectrometry-grade protease with PTM-directed specificity.

PubMed

Tran, Duc T; Cavett, Valerie J; Dang, Vuong Q; Torres, Héctor L; Paegel, Brian M

2016-12-20

Mapping posttranslational modifications (PTMs), which diversely modulate biological functions, represents a significant analytical challenge. The centerpiece technology for PTM site identification, mass spectrometry (MS), requires proteolytic cleavage in the vicinity of a PTM to yield peptides for sequencing. This requirement catalyzed our efforts to evolve MS-grade mutant PTM-directed proteases. Citrulline, a PTM implicated in epigenetic and immunological function, made an ideal first target, because citrullination eliminates arginyl tryptic sites. Bead-displayed trypsin mutant genes were translated in droplets, the mutant proteases were challenged to cleave bead-bound fluorogenic probes of citrulline-dependent proteolysis, and the resultant beads (1.3 million) were screened. The most promising mutant efficiently catalyzed citrulline-dependent peptide bond cleavage (k cat /K M = 6.9 × 10 5 M -1 ⋅s -1 ). The resulting C-terminally citrullinated peptides generated characteristic isotopic patterns in MALDI-TOF MS, and both a fragmentation product y 1 ion corresponding to citrulline (176.1030 m/z) and diagnostic peak pairs in the extracted ion chromatograms of LC-MS/MS analysis. Using these signatures, we identified citrullination sites in protein arginine deiminase 4 (12 sites) and in fibrinogen (25 sites, two previously unknown). The unique mass spectral features of PTM-dependent proteolytic digest products promise a generalized PTM site-mapping strategy based on a toolbox of such mutant proteases, which are now accessible by laboratory evolution.

Nest-site selection and nest success of an Arctic-breeding passerine, Smith's Longspur, in a changing climate

USGS Publications Warehouse

McFarland, Heather R.; Kendall, Steve J.; Powell, Abby

2017-01-01

Despite changes in shrub cover and weather patterns associated with climate change in the Arctic, little is known about the breeding requirements of most passerines tied to northern regions. We investigated the nesting biology and nest habitat characteristics of Smith's Longspurs (Calcarius pictus) in 2 study areas in the Brooks Range of Alaska, USA. First, we examined variation in nesting phenology in relation to local temperatures. We then characterized nesting habitat and analyzed nest-site selection for a subset of nests (n = 86) in comparison with paired random points. Finally, we estimated the daily survival rate of 257 nests found in 2007–2013 with respect to both habitat characteristics and weather variables. Nest initiation was delayed in years with snow events, heavy rain, and freezing temperatures early in the breeding season. Nests were typically found in open, low-shrub tundra, and never among tall shrubs (mean shrub height at nests = 26.8 ± 6.7 cm). We observed weak nest-site selection patterns. Considering the similarity between nest sites and paired random points, coupled with the unique social mating system of Smith's Longspurs, we suggest that habitat selection may occur at the neighborhood scale and not at the nest-site scale. The best approximating model explaining nest survival suggested a positive relationship with the numbers of days above 21°C that an individual nest experienced; there was little support for models containing habitat variables. The daily nest survival rate was high (0.972–0.982) compared with that of most passerines in forested or grassland habitats, but similar to that of passerines nesting on tundra. Considering their high nesting success and ability to delay nest initiation during inclement weather, Smith's Longspurs may be resilient to predicted changes in weather regimes on the breeding grounds. Thus, the greatest threat to breeding Smith's Longspurs associated with climate change may be the loss of low-shrub habitat types, which could significantly change the characteristics of breeding areas.

The useful potential of using existing data to uniquely identify predictable wind events and regimes, part 1

NASA Technical Reports Server (NTRS)

Trettel, D. W.; Aquino, J. T.; Piazza, T. R.; Taylor, L. E.; Trask, D. C.

1982-01-01

Correlations between standard meteorological data and wind power generation potential were developed. Combined with appropriate wind forecasts, these correlations can be useful to load dispatchers to supplement conventional energy sources. Hourly wind data were analyzed for four sites, each exhibiting a unique physiography. These sites are Amarillo, Texas; Ludington, Michigan; Montauk Point, New York; and San Gorgonio, California. Synoptic weather maps and tables are presented to illustrate various wind 'regimes' at these sites.

VISA--Vector Integration Site Analysis server: a web-based server to rapidly identify retroviral integration sites from next-generation sequencing.

PubMed

Hocum, Jonah D; Battrell, Logan R; Maynard, Ryan; Adair, Jennifer E; Beard, Brian C; Rawlings, David J; Kiem, Hans-Peter; Miller, Daniel G; Trobridge, Grant D

2015-07-07

Analyzing the integration profile of retroviral vectors is a vital step in determining their potential genotoxic effects and developing safer vectors for therapeutic use. Identifying retroviral vector integration sites is also important for retroviral mutagenesis screens. We developed VISA, a vector integration site analysis server, to analyze next-generation sequencing data for retroviral vector integration sites. Sequence reads that contain a provirus are mapped to the human genome, sequence reads that cannot be localized to a unique location in the genome are filtered out, and then unique retroviral vector integration sites are determined based on the alignment scores of the remaining sequence reads. VISA offers a simple web interface to upload sequence files and results are returned in a concise tabular format to allow rapid analysis of retroviral vector integration sites.

Soil Microbial Forensics.

PubMed

Santiago-Rodriguez, Tasha M; Cano, Raúl J

2016-08-01

Soil microbial forensics can be defined as the study of how microorganisms can be applied to forensic investigations. The field of soil microbial forensics is of increasing interest and applies techniques commonly used in diverse disciplines in order to identify microbes and determine their abundances, complexities, and interactions with soil and surrounding objects. Emerging new techniques are also providing insights into the complexity of microbes in soil. Soil may harbor unique microbes that may reflect specific physical and chemical characteristics indicating site specificity. While applications of some of these techniques in the field of soil microbial forensics are still in early stages, we are still gaining insight into how microorganisms may be more robustly used in forensic investigations.

Systems of care for individuals with autism spectrum disorder and serious behavioral disturbance through the lifespan.

PubMed

Lubetsky, Martin J; Handen, Benjamin L; Lubetsky, Michelle; McGonigle, John J

2014-01-01

Individuals with Autism Spectrum Disorder present with unique characteristics, and the interventions designed to address associated challenging behaviors must be highly individualized to best meet their needs and those of their families. This article reviews systems of care to support the child, adolescent, or adult with Autism Spectrum Disorder and/or Intellectual Disability. The review describes mental health/behavioral health services, Intellectual Disability and other support systems, and the systems involved in a child and adolescent's life and transition to adulthood. The types of systems and services, as well as barriers, are delineated with a brief listing of Web sites and references. Copyright © 2014 Elsevier Inc. All rights reserved.

The rules of engagement: comparing two social protest movements on YouTube.

PubMed

Vraga, Emily K; Bode, Leticia; Wells, Chris; Driscoll, Kevin; Thorson, Kjerstin

2014-03-01

Social media Web sites such as YouTube offer activists unique opportunities to reach out to new audiences through a variety of diverse appeals. Yet the rules of engagement on social media should depend on the structures, goals, and characteristics of the movements engaging in this outreach. To explore how differences in social movements translate into online activism, we employ a paired case study approach, comparing YouTube artifacts for two political mobilizations: the Occupy Movement and California's Proposition 8 ballot initiative concerning same sex marriage. Across movements, we examine the popularity of videos and their characteristics, and whether the type of video consistently predicts video engagement. We find that "social media activism" is not a unitary phenomenon; the two mobilizations produced unique YouTube ecologies. Occupy Wall Street videos tended on average to produce less engagement and focused on filmed live events and amateur content. Meanwhile, Proposition 8 videos usually produced more engagement and bridged more diverse formats: from professionalized and scripted content to live event footage and unscripted monologues to the camera. Therefore, our study suggests that social activism in online spaces such as YouTube is not easily defined, but is adapted to suit movement needs-which makes social media a popular and flexible venue for activism but also highlights the challenges for scholars studying such venues.

76 FR 23333 - Notice of Proposed Withdrawal Extension and Opportunity for Public Meeting; Wyoming

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-26

... Land Management, Interior. ACTION: Notice. SUMMARY: The United States Department of Agriculture (USDA... mining laws to protect unique topographic characteristics and recreation values of the Snowy Range Area... withdrawal extension is to continue to protect the unique topographic characteristics of the Snowy Range Area...

Optimizing Restriction Site Placement for Synthetic Genomes

NASA Astrophysics Data System (ADS)

Montes, Pablo; Memelli, Heraldo; Ward, Charles; Kim, Joondong; Mitchell, Joseph S. B.; Skiena, Steven

Restriction enzymes are the workhorses of molecular biology. We introduce a new problem that arises in the course of our project to design virus variants to serve as potential vaccines: we wish to modify virus-length genomes to introduce large numbers of unique restriction enzyme recognition sites while preserving wild-type function by substitution of synonymous codons. We show that the resulting problem is NP-Complete, give an exponential-time algorithm, and propose effective heuristics, which we show give excellent results for five sample viral genomes. Our resulting modified genomes have several times more unique restriction sites and reduce the maximum gap between adjacent sites by three to nine-fold.

Low-flow characteristics of streams in Virginia

USGS Publications Warehouse

Hayes, Donald C.

1991-01-01

Streamflow data were collected and low-flow characteristics computed for 715 gaged sites in Virginia Annual minimum average 7-consecutive-day flows range from 0 to 2,195 cubic feet per second for a 2-year recurrence interval and from 0 to 1,423 cubic feet per second for a 10-year recurrence interval. Drainage areas range from 0.17 to 7,320 square miles. Existing and discontinued gaged sites are separated into three types: long-term continuous-record sites, short-term continuous-record sites, and partial-record sites. Low-flow characteristics for long-term continuous-record sites are determined from frequency curves of annual minimum average 7-consecutive-day flows . Low-flow characteristics for short-term continuous-record sites are estimated by relating daily mean base-flow discharge values at a short-term site to concurrent daily mean discharge values at nearby long-term continuous-record sites having similar basin characteristics . Low-flow characteristics for partial-record sites are estimated by relating base-flow measurements to daily mean discharge values at long-term continuous-record sites. Information from the continuous-record sites and partial-record sites in Virginia are used to develop two techniques for estimating low-flow characteristics at ungaged sites. A flow-routing method is developed to estimate low-flow values at ungaged sites on gaged streams. Regional regression equations are developed for estimating low-flow values at ungaged sites on ungaged streams. The flow-routing method consists of transferring low-flow characteristics from a gaged site, either upstream or downstream, to a desired ungaged site. A simple drainage-area proration is used to transfer values when there are no major tributaries between the gaged and ungaged sites. Standard errors of estimate for108 test sites are 19 percent of the mean for estimates of low-flow characteristics having a 2-year recurrence interval and 52 percent of the mean for estimates of low-flow characteristics having a 10-year recurrence interval . A more complex transfer method must be used when major tributaries enter the stream between the gaged and ungaged sites. Twenty-four stream networks are analyzed, and predictions are made for 84 sites. Standard errors of estimate are 15 percent of the mean for estimates of low-flow characteristics having a 2-year recurrence interval and 22 percent of the mean for estimates of low-flow characteristics having a 10-year recurrence interval. Regional regression equations were developed for estimating low-flow values at ungaged sites on ungaged streams. The State was divided into eight regions on the basis of physiography and geographic grouping of the residuals computed in regression analyses . Basin characteristics that were significant in the regression analysis were drainage area, rock type, and strip-mined area. Standard errors of prediction range from 60 to139 percent for estimates of low-flow characteristics having a 2-year recurrence interval and 90 percent to 172 percent for estimates of low-flow characteristics having a 10-year recurrence interval.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Armstrong, Christopher R.; Brant, Heather A.; Nuessle, Patterson R.

Owing to the rich history of heavy element production in the unique high flux reactors that operated at the Savannah River Site, USA (SRS) decades ago, trace quantities of plutonium with highly unique isotopic characteristics still persist today in the SRS terrestrial environment. Development of an effective sampling, processing, and analysis strategy enables detailed monitoring of the SRS environment, revealing plutonium isotopic compositions, e.g., 244Pu, that reflect the unique legacy of plutonium production at SRS. This work describes the first long-term investigation of anthropogenic 244Pu occurrence in the environment. Environmental samples, consisting of collected foot borne debris, were taken atmore » SRS over an eleven year period, from 2003 to 2014. Separation and purification of trace plutonium was carried out followed by three stage thermal ionization mass spectrometry (3STIMS) measurements for plutonium isotopic content and isotopic ratios. Furthermore, significant 244Pu was measured in all of the years sampled with the highest amount observed in 2003. The 244Pu content, in femtograms (fg = 10 –15 g) per gram, ranged from 0.31 fg/g to 44 fg/g in years 2006 and 2003 respectively. In all years, the 244Pu/ 239Pu atom ratios were significantly higher than global fallout, ranging from 0.003 to 0.698 in years 2014 and 2003 respectively.« less

Construction safety monitoring based on the project's characteristic with fuzzy logic approach

NASA Astrophysics Data System (ADS)

Winanda, Lila Ayu Ratna; Adi, Trijoko Wahyu; Anwar, Nadjadji; Wahyuni, Febriana Santi

2017-11-01

Construction workers accident is the highest number compared with other industries and falls are the main cause of fatal and serious injuries in high rise projects. Generally, construction workers accidents are caused by unsafe act and unsafe condition that can occur separately or together, thus a safety monitoring system based on influencing factors is needed to achieve zero accident in construction industry. The dynamic characteristic in construction causes high mobility for workers while doing the task, so it requires a continuously monitoring system to detect unsafe condition and to protect workers from potential hazards. In accordance with the unique nature of project, fuzzy logic approach is one of the appropriate methods for workers safety monitoring on site. In this study, the focus of discussion is based on the characteristic of construction projects in analyzing "potential hazard" and the "protection planning" to be used in accident prevention. The data have been collected from literature review, expert opinion and institution of safety and health. This data used to determine hazard identification. Then, an application model is created using Delphi programming. The process in fuzzy is divided into fuzzification, inference and defuzzification, according to the data collection. Then, the input and final output data are given back to the expert for assessment as a validation of application model. The result of the study showed that the potential hazard of construction workers accident could be analysed based on characteristic of project and protection system on site and fuzzy logic approach can be used for construction workers accident analysis. Based on case study and the feedback assessment from expert, it showed that the application model can be used as one of the safety monitoring tools.

Life-history and habitat features influence the within-river genetic structure of Atlantic salmon.

PubMed

Vähä, Juha-Pekka; Erkinaro, Jaakko; Niemelä, Eero; Primmer, Craig R

2007-07-01

Defining populations and identifying ecological and life-history characteristics affecting genetic structure is important for understanding species biology and hence, for managing threatened or endangered species or populations. In this study, populations of the world's largest indigenous Atlantic salmon (Salmo salar) stock were first inferred using model-based clustering methods, following which life-history and habitat variables best predicting the genetic diversity of populations were identified. This study revealed that natal homing of Atlantic salmon within the Teno River system is accurate at least to the tributary level. Generally, defining populations by main tributaries was observed to be a reasonable approach in this large river system, whereas in the mainstem of the river, the number of inferred populations was fewer than the number of distinct sampling sites. Mainstem and headwater populations were genetically more diverse and less diverged, while each tributary fostered a distinct population with high genetic differentiation and lower genetic diversity. Population structure and variation in genetic diversity among populations were poorly explained by geographical distance. In contrast, age-structure, as estimated by the proportion of multisea-winter spawners, was the most predictive variable in explaining the variation in the genetic diversity of the populations. This observation, being in agreement with theoretical predictions, emphasizes the essence of large multisea-winter females in maintaining the genetic diversity of populations. In addition, the unique genetic diversity of populations, as estimated by private allele richness, was affected by the ease of accessibility of a site, with more difficult to access sites having lower unique genetic diversity. Our results show that despite this species' high capacity for migration, tributaries foster relatively closed populations with little gene flow which will be important to consider when developing management strategies for the system.

Management of climatic heat stress risk in construction: a review of practices, methodologies, and future research.

PubMed

Rowlinson, Steve; Yunyanjia, Andrea; Li, Baizhan; Chuanjingju, Carrie

2014-05-01

Climatic heat stress leads to accidents on construction sites brought about by a range of human factors emanating from heat induced illness, and fatigue leading to impaired capability, physical and mental. It is an occupational characteristic of construction work in many climates and the authors take the approach of re-engineering the whole safety management system rather than focusing on incremental improvement, which is current management practice in the construction industry. From a scientific viewpoint, climatic heat stress is determined by six key factors: (1) air temperature, (2) humidity, (3) radiant heat, and (4) wind speed indicating the environment, (5) metabolic heat generated by physical activities, and (6) "clothing effect" that moderates the heat exchange between the body and the environment. By making use of existing heat stress indices and heat stress management processes, heat stress risk on construction sites can be managed in three ways: (1) control of environmental heat stress exposure through use of an action-triggering threshold system, (2) control of continuous work time (CWT, referred by maximum allowable exposure duration) with mandatory work-rest regimens, and (3) enabling self-paced working through empowerment of employees. Existing heat stress practices and methodologies are critically reviewed and the authors propose a three-level methodology for an action-triggering, localized, simplified threshold system to facilitate effective decisions by frontline supervisors. The authors point out the need for "regional based" heat stress management practices that reflect unique climatic conditions, working practices and acclimatization propensity by local workers indifferent geographic regions. The authors set out the case for regional, rather than international, standards that account for this uniqueness and which are derived from site-based rather than laboratory-based research. Copyright © 2013 Elsevier Ltd. All rights reserved.

The Family Environment and Developmental Psychopathology: The Unique and Interactive Effects of Depression, Attention, and Conduct Problems

ERIC Educational Resources Information Center

George, Carrie; Herman, Keith C.; Ostrander, Rick

2006-01-01

Prior studies have found remarkable similarity in the family characteristics across a wide range of child psychopathologies. This study investigated the unique relationships between symptoms of depression, conduct problems/aggression, and inattention/hyperactivity and characteristics of the family environment. Parents and teachers completed…

Functional Studies on Oligotropha carboxidovorans Molybdenum-Copper CO Dehydrogenase Produced in Escherichia coli.

PubMed

Kaufmann, Paul; Duffus, Benjamin R; Teutloff, Christian; Leimkühler, Silke

2018-04-30

The Mo/Cu-dependent CO dehydrogenase (CODH) from Oligotropha carboxidovorans is an enzyme that is able to catalyze both the oxidation of CO to CO 2 and the oxidation of H 2 to protons and electrons. Despite the close to atomic resolution structure (1.1 Å), significant uncertainties have remained with regard to the reaction mechanism of substrate oxidation at the unique Mo/Cu center, as well as the nature of intermediates formed during the catalytic cycle. So far, the investigation of the role of amino acids at the active site was hampered by the lack of a suitable expression system that allowed for detailed site-directed mutagenesis studies at the active site. Here, we report on the establishment of a functional heterologous expression system of O. carboxidovorans CODH in Escherichia coli. We characterize the purified enzyme in detail by a combination of kinetic and spectroscopic studies and show that it was purified in a form with characteristics comparable to those of the native enzyme purified from O. carboxidovorans. With this expression system in hand, we were for the first time able to generate active-site variants of this enzyme. Our work presents the basis for more detailed studies of the reaction mechanism for CO and H 2 oxidation of Mo/Cu-dependent CODHs in the future.

Targeting the lymphatics using dendritic polymers (dendrimers).

PubMed

Kaminskas, Lisa M; Porter, Christopher J H

2011-09-10

Dendrimers are unique biomaterials that are constructed by the stepwise addition of layers (generations) of polymer around a central core. They can be constructed with a range of molecular weights and have a polyfunctional surface that facilitates the attachment of drugs and pharmacokinetic modifiers such PEG or targeting moieties. These properties have led to considerable interest in the development of dendrimers for a range of biomedical applications. After subcutaneous administration, larger dendrimers in particular (> 8 nm), preferentially drain from the injection site into the peripheral lymphatic capillaries and therefore have potential as lymphatic imaging agents for magnetic resonance and optical fluorescence lymphangiography and as vectors for drug-targeting to lymphatic sites of disease progression. In general, lymphatic targeting of dendrimers is enhanced by increasing size although ultimately larger constructs may be incompletely absorbed from the injection site. Increasing hydrophilicity and reducing surface charge enhances drainage from subcutaneous injection sites, but the reverse is true of uptake into lymph nodes where charge and hydrophobicity promote retention. Larger hydrophilic dendrimers are also capable of extravasation from the systemic circulation, absorption into the lymphatic system and recirculation into the blood. Lymphatic recirculation may therefore be a characteristic of PEGylated dendrimers with long systemic circulation times. Copyright © 2011 Elsevier B.V. All rights reserved.

Comparison of mosquito control programs in seven urban sites in Africa, the Middle East, and the Americas

PubMed Central

Impoinvil, Daniel E.; Ahmad, Sajjad; Troyo, Adriana; Keating, Joseph; Githeko, Andrew K.; Mbogo, Charles M; Kibe, Lydiah; Githure, John I.; Gad, Adel M.; Hassan, Ali N.; Orshan, Laor; Warburg, Alon; Calderón-Arguedas, Olger; Sánchez-Loría, Victoria M.; Velit-Suarez, Rosanna; Chadee, Dave D.; Novak, Robert J.; Beier, John C.

2007-01-01

Mosquito control programs at seven urban sites in Kenya, Egypt, Israel, Costa Rica, and Trinidad are described and compared. Site-specific urban and disease characteristics, organizational diagrams, and strengths, weaknesses, obstacles and threats (SWOT) analysis tools are used to provide a descriptive assessment of each mosquito control program, and provide a comparison of the factors affecting mosquito abatement. The information for SWOT analysis is collected from surveys, focus group discussions, and personal communication. SWOT analysis identified various issues affecting the efficiency and sustainability of mosquito control operations. The main outcome of our work was the description and comparison of mosquito control operations within the context of each study site’s biological, social, political, management, and economic conditions. The issues identified in this study ranged from lack of inter-sector collaboration to operational issues of mosquito control efforts. A lack of sustainable funding for mosquito control was a common problem for most sites. Many unique problems were also identified, which included lack of mosquito surveillance, lack of law enforcement, and negative consequences of human behavior. Identifying common virtues and shortcomings of mosquito control operations is useful in identifying “best practices” for mosquito control operations, thus leading to better control of mosquito biting and mosquito-borne disease transmission. PMID:17316882

Factors influencing unsafe behaviors and accidents on construction sites: a review.

PubMed

Khosravi, Yahya; Asilian-Mahabadi, Hassan; Hajizadeh, Ebrahim; Hassanzadeh-Rangi, Narmin; Bastani, Hamid; Behzadan, Amir H

2014-01-01

Construction is a hazardous occupation due to the unique nature of activities involved and the repetitiveness of several field behaviors. The aim of this methodological and theoretical review is to explore the empirical factors influencing unsafe behaviors and accidents on construction sites. In this work, results and findings from 56 related previous studies were investigated. These studies were categorized based on their design, type, methods of data collection, analytical methods, variables, and key findings. A qualitative content analysis procedure was used to extract variables, themes, and factors. In addition, all studies were reviewed to determine the quality rating and to evaluate the strength of provided evidence. The content analysis identified 8 main categories: (a) society, (b) organization, (c) project management, (d) supervision, (e) contractor, (f) site condition, (g) work group, and (h) individual characteristics. The review highlighted the importance of more distal factors, e.g., society and organization, and project management, that may contribute to reducing the likelihood of unsafe behaviors and accidents through the promotion of site condition and individual features (as proximal factors). Further research is necessary to provide a better understanding of the links between unsafe behavior theories and empirical findings, challenge theoretical assumptions, develop new applied theories, and make stronger recommendations.

Physical, Chemical, and Biological Methods and Data from the Urban Land-Use-Gradient Study, Des Plaines and Fox River Basins, Illinois, 1999-2001

USGS Publications Warehouse

Adolphson, Debbie L.; Arnold, Terri L.; Fitzpatrick, Faith A.; Harris, Mitchell A.; Richards, Kevin D.; Scudder, Barbara C.; Stewart, Jana S.

2001-01-01

Physical, chemical, and biological data were collected at 46 sites in the Fox and Des Plaines River Basins as part of the upper Illinois River Basin study of the U.S. Geological Survey?s National Water-Quality Assessment Program. The data, collected from 1999 to 2001, will be used to determine the effects of urbanization on streams in the Chicago, Illinois, metropolitan area. To examine the possible effects of urbanization on stream-water quality, the sampling sites were selected to represent a gradient of land use changing from agriculture into urban. Urban land use for the selected sites ranged from less than 1 percent urban to 92 percent urban. Data-collection methods are presented in the text portion of this report. Physical characteristics of the stream that were collected include descriptive and qualitative habitat and geomorphic measures. Water samples were analyzed for nutrients (nitrogen and phosphorus), 11 major ions, 46 wastewater indicators, pH, and specific conductance. Aquatic communities were sampled to identify and quantify populations of selected algae, benthic macroinvertebrates, and fish. There were 72 unique fish species collected at all of the sites. The number of benthic macroinvertebrate taxa collected at all the sites ranged from 15 to 48. The data and the associated data documentation are presented on a CD-ROM included with this report.

Homo floresiensis and the late Pleistocene environments of eastern Indonesia: defining the nature of the relationship

NASA Astrophysics Data System (ADS)

Westaway, K. E.; Morwood, M. J.; Sutikna, T.; Moore, M. W.; Rokus, A. D.; van den Bergh, G. D.; Roberts, R. G.; Saptomo, E. W.

2009-12-01

Evidence from Liang Bua, a limestone cave on the island of Flores in East Indonesia, provides a unique opportunity to explore the long term relationship between hominins and their environment. Occupation deposits at the site span ˜95 ka and contain abundant stone artefacts, well preserved faunal remains and evidence for an endemic species of hominin: Homo floresiensis. Work at the site included detailed geomorphological and environmental analysis, which has enabled comparisons to be drawn between changes in the occupational intensity in the cave, using stone tool and faunal counts, and changes in the environmental conditions, using the characteristics of the sedimentary layers in the cave and speleothem records. These comparisons demonstrate that H. floresiensis endured rapidly fluctuating environmental conditions over the last ˜100 ka, which influenced the geomorphological processes in the cave and their occupational conditions. The intensity of occupation in the cave changed significantly between 95 and 17 ka, with peaks in occupation occurring at 100-95, 74-61 and 18-17 ka. These correlate with episodes of channel formation and erosion in the cave, which in turn correspond with high rainfall, thick soils and high bio-productivity outside. In contrast, periods of low occupational intensity correlate with reduced channel activity and pooling associated with drier periods from 94 to 75 and 36 to 19 ka. This apparent link between intensity of hominin use of the cave and the general conditions outside relates to the expansion and contraction of the rainforest and the ability of H. floresiensis to adapt to habitat changes. This interpretation implies that these diminutive hominins were able to survive abrupt and prolonged environmental changes by changing their favoured occupation sites. These data provide the basis for a model of human-environment interactions on the island of Flores. With the addition of extra data from other sites on Flores, this model will provide a greater understanding of H. floresiensis as a unique human species.

Crystal structure of class III chitinase from pomegranate provides the insight into its metal storage capacity.

PubMed

Masuda, Taro; Zhao, Guanghua; Mikami, Bunzo

2015-01-01

Chitinase hydrolyzes the β-1,4-glycosidic bond in chitin. In higher plants, this enzyme has been regarded as a pathogenesis-related protein. Recently, we identified a class III chitinase, which functions as a calcium storage protein in pomegranate (Punica granatum) seed (PSC, pomegranate seed chitinase). Here, we solved a crystal structure of PSC at 1.6 Å resolution. Although its overall structure, including the structure of catalytic site and non-proline cis-peptides, was closely similar to those of other class III chitinases, PSC had some unique structural characteristics. First, there were some metal-binding sites with coordinated water molecules on the surface of PSC. Second, many unconserved aspartate residues were present in the PSC sequence which rendered the surface of PSC negatively charged. This acidic electrostatic property is in contrast to that of hevamine, well-characterized plant class III chitinase, which has rather a positively charged surface. Thus, the crystal structure provides a clue for metal association property of PSC.

High Temperature and High Pressure Mixtures of Iron Oxides from the Impact Event at the Bee Bluff Crypto-Meteorite Impact Crater of South Texas

NASA Astrophysics Data System (ADS)

Graham, R. A.

2012-10-01

Disturbed geology within a several km diameter surface area of sedimentary Carrizo Sandstone near Uvalde, Texas, indicates the presence of a partially buried meteorite impact crater. Identification of its impact origin is supported by detailed studies but quartz grains recovered from distances of about100 km from the structure also show planar deformation features (PDFs). While PDFs are recognized as uniquely from impact processes, quantitative interpretation requires extension of Hugoniot materials models to more realistic grain-level, mixture models. Carrizo sandstone is a porous mixture of fine quartz and goethite. At impact pressures of tens of GPa, goethite separates into hematite and water vapor upon release of impact pressure. Samples from six different locations up to 50 km from the impact site preserve characteristic features resulting from mixtures of goethite, its water vapor, hematite and quartz. Spheroids resulting from local radial acceleration of mixed density, hot products are common at various sites. Local hydrodynamic instabilities cause similar effects.

High-Performance Simulations of the Diffusion Characteristics of a Pentacene Derivative on Gold Surfaces

NASA Astrophysics Data System (ADS)

Miller, Ryan; Larson, Amanda; Pohl, Karsten

Pentacene serves as a backbone for several molecules that provide attractive qualities for organic photovoltaic devices. One of these pentacene derivatives is 5 6,7-trithiapentacene-13-one (TTPO), which is unique in that it achieves its lowest energy configuration on Au(1 1 1) surfaces with the thiol group angled down towards the surface, allowing many molecules to pack closely together and form molecular nanowires. However, TTPO diffuses on flat surfaces, making it difficult for the self-assembly process to be initiated. With the help of the low-energy sites in surface defects and Au(7 8 8) step edges, TTPO molecules can be anchored in place on surfaces, allowing for chain formation to begin. By using high-performance Density Functional Theory based molecular dynamics calculations, the molecules can be shown to stay localized to these bonding sites and serve as a basis for chain formation. In addition, by simulating various temperatures with a Nose-Hoover thermostat, we can analyze how temperature affects anchoring ability and diffusion properties.

Estimates of monthly streamflow characteristics at selected sites in the upper Missouri River basin, Montana, base period water years 1937-86

USGS Publications Warehouse

Parrett, Charles; Johnson, D.R.; Hull, J.A.

1989-01-01

Estimates of streamflow characteristics (monthly mean flow that is exceeded 90, 80, 50, and 20 percent of the time for all years of record and mean monthly flow) were made and are presented in tabular form for 312 sites in the Missouri River basin in Montana. Short-term gaged records were extended to the base period of water years 1937-86, and were used to estimate monthly streamflow characteristics at 100 sites. Data from 47 gaged sites were used in regression analysis relating the streamflow characteristics to basin characteristics and to active-channel width. The basin-characteristics equations, with standard errors of 35% to 97%, were used to estimate streamflow characteristics at 179 ungaged sites. The channel-width equations, with standard errors of 36% to 103%, were used to estimate characteristics at 138 ungaged sites. Streamflow measurements were correlated with concurrent streamflows at nearby gaged sites to estimate streamflow characteristics at 139 ungaged sites. In a test using 20 pairs of gages, the standard errors ranged from 31% to 111%. At 139 ungaged sites, the estimates from two or more of the methods were weighted and combined in accordance with the variance of individual methods. When estimates from three methods were combined the standard errors ranged from 24% to 63 %. A drainage-area-ratio adjustment method was used to estimate monthly streamflow characteristics at seven ungaged sites. The reliability of the drainage-area-ratio adjustment method was estimated to be about equal to that of the basin-characteristics method. The estimate were checked for reliability. Estimates of monthly streamflow characteristics from gaged records were considered to be most reliable, and estimates at sites with actual flow record from 1937-86 were considered to be completely reliable (zero error). Weighted-average estimates were considered to be the most reliable estimates made at ungaged sites. (USGS)

The VA Ostomy Health-Related Quality of Life Study: objectives, methods, and patient sample.

PubMed

Krouse, Robert S; Mohler, M Jane; Wendel, Christopher S; Grant, Marcia; Baldwin, Carol M; Rawl, Susan M; McCorkle, Ruth; Rosenfeld, Kenneth E; Ko, Clifford Y; Schmidt, C Max; Coons, Stephen Joel

2006-04-01

To present the design and methods of a multisite study of health-related quality of life (HR-QOL) in veterans living with ostomies. Veterans from Tucson, Indianapolis, and Los Angeles VA Medical Centers were surveyed using the validated City of Hope ostomy-specific tool (mCOH-QOL-Ostomy) and the SF-36V. Cases (ostomates) had a major gastrointestinal procedure that required an intestinal stoma, while controls had similar procedures for which an ostomy was not required. Ostomy subjects were recruited for four focus groups in each of two sites divided by ostomy type (colostomy versus ileostomy) and overall mCOH-QOL-Ostomy HR-QOL score (highest versus lowest quartile). The focus groups further evaluated barriers, concerns, and adaptation methods and skills. This report presents recruitment results, reliability of survey instruments, and demographic characteristics of the sample. The overall response (i.e., recruitment) rate across all sites was 48% and by site was 53%, 57%, and 37%, respectively (p < 0.001). Internal consistency reliability estimates indicated that both instruments remain reliable in this population (Cronbach's alpha for HR-QOL domains/scales: 0.71-0.96). Cases and controls were similar in demographic characteristics. Proportions of minority subjects matched projections from the site patient populations. Subjects with ostomies had significantly longer time since surgery than controls (p < 0.001). Focus groups were comprised of two to six subjects per group and demonstrated racial diversity at the Los Angeles site. The unique design of our study of VA patients with ostomies is an illustration of a successful mixed methods approach to HR-QOL research. We collected meaningful quantitative and qualitative data that will be used in the development of new approaches to care that will lead to improved functioning and well-being in persons living with ostomies. Subsequent reports will provide the results of this research project.

Population dynamics of Aedes aegypti from a dengue hyperendemic urban setting in Colombia.

PubMed

Ocampo, Clara B; Wesson, Dawn M

2004-10-01

This study evaluated if the Aedes aegypti population in the city of Cali, Colombia was composed of genetically distinct local populations with different levels of insecticide resistance and dengue vector competence. Insecticide resistance was assayed biochemically and was associated with varying levels of mixed-function oxidases and non-specific esterases. The genes encoding those enzymes were under selective pressure from insecticides used to suppress Ae. aegypti populations. Vector competence showed heterogeneity among the vector populations ranging from 19% to 60%. Population genetic analysis of random amplified polymorphic DNA-polymerase chain reaction products, expressed as genetic distance, Wright's F(st), and migration rate (Nm), demonstrated moderate genetic differentiation among Ae. aegypti from four sites (F(st) = 0.085). The results from all characteristics evaluated in the study demonstrated spatial and temporal variation between Ae. aegypti populations. At any specific time, the local populations of Ae. aegypti were genetically differentiated and unique with respect to insecticide resistance and vector competence. Both characteristics changed independently.

Longitudinal Distribution of the Functional Feeding Groups of Aquatic Insects in Streams of the Brazilian Cerrado Savanna.

PubMed

Brasil, L S; Juen, L; Batista, J D; Pavan, M G; Cabette, H S R

2014-10-01

We demonstrate that the distribution of the functional feeding groups of aquatic insects is related to hierarchical patch dynamics. Patches are sites with unique environmental and functional characteristics that are discontinuously distributed in time and space within a lotic system. This distribution predicts that the occurrence of species will be based predominantly on their environmental requirements. We sampled three streams within the same drainage basin in the Brazilian Cerrado savanna, focusing on waterfalls and associated habitats (upstream, downstream), representing different functional zones. We collected 2,636 specimens representing six functional feeding groups (FFGs): brushers, collector-gatherers, collector-filterers, shredders, predators, and scrapers. The frequency of occurrence of these groups varied significantly among environments. This variation appeared to be related to the distinct characteristics of the different habitat patches, which led us to infer that the hierarchical patch dynamics model can best explain the distribution of functional feeding groups in minor lotic environments, such as waterfalls.

Historical space psychology: Early terrestrial explorations as Mars analogues

NASA Astrophysics Data System (ADS)

Suedfeld, Peter

2010-03-01

The simulation and analogue environments used by psychologists to circumvent the difficulties of conducting research in space lack many of the unique characteristics of future explorations, especially the mission to Mars. This paper suggests that appropriate additional analogues would be the multi-year maritime and terrestrial explorations that mapped the surface of the Earth in previous centuries. These, like Mars, often involved a hazardous trek through unknown territory, flanked by extended, dangerous voyages to and from the exploration sites. Characteristic issues included interpersonal relationships under prolonged stress, stretches of boredom interspersed with intense work demands, the impossibility of rescue, resupply, or other help from home, chronic danger, physical discomfort and lack of privacy, and the crucial role of the leader. Illustrative examples of one important factor, leadership style, are discussed. The examination of such expeditions can help to identify the psychological stressors that are likely to be experienced by Mars explorers, and can also indicate countermeasures to reduce the damaging impact of those stressors.

The structure of myostatin:follistatin 288: insights into receptor utilization and heparin binding

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cash, Jennifer N.; Rejon, Carlis A.; McPherron, Alexandra C.

2009-09-29

Myostatin is a member of the transforming growth factor-{beta} (TGF-{beta}) family and a strong negative regulator of muscle growth. Here, we present the crystal structure of myostatin in complex with the antagonist follistatin 288 (Fst288). We find that the prehelix region of myostatin very closely resembles that of TGF-{beta} class members and that this region alone can be swapped into activin A to confer signalling through the non-canonical type I receptor Alk5. Furthermore, the N-terminal domain of Fst288 undergoes conformational rearrangements to bind myostatin and likely acts as a site of specificity for the antagonist. In addition, a unique continuousmore » electropositive surface is created when myostatin binds Fst288, which significantly increases the affinity for heparin. This translates into stronger interactions with the cell surface and enhanced myostatin degradation in the presence of either Fst288 or Fst315. Overall, we have identified several characteristics unique to myostatin that will be paramount to the rational design of myostatin inhibitors that could be used in the treatment of muscle-wasting disorders.« less

Japanese encephalitis virus NS1' protein depends on pseudoknot secondary structure and is cleaved by caspase during virus infection and cell apoptosis.

PubMed

Sun, Jin; Yu, Yongxin; Deubel, Vincent

2012-09-01

Japanese encephalitis virus (JEV) is a flavivirus with a complex life cycle involving mosquito vectors that mainly target birds and pigs, and causes severe encephalitis in children in Asia. Neurotropic flaviviruses of the JEV serogroup have a particular characteristic of expressing a unique nonstructural NS1' protein, which is a prolongation of NS1 at the C terminus by 52 amino acids derived from a pseudoknot-driven-1 translation frameshift. Protein NS1' is associated with virus neuro-invasiveness. In this study, the need of the pseudoknot structure for NS1' synthesis was confirmed. By using a specific antibody against the prolonged peptide, NS1' was found to be absent from the JEV SA14-14-2 vaccine strain, resulting from a single nucleotide silent mutation in the pseudoknot. A partial cleavage of NS1' at a specific site of its C-terminal appendix recognized by caspases and inhibited by caspase inhibitors suggests a unique feature of intracellular NS1'. Copyright © 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

A single R36Q mutation in the matrix protein of pigeon paramyxovirus type 1 reduces virus replication and shedding in pigeons.

PubMed

Xu, Haixu; Song, Qingqing; Zhu, Jie; Liu, Jiajia; Cheng, Xin; Hu, Shunlin; Wu, Shuang; Wang, Xiaoquan; Liu, Xiaowen; Liu, Xiufan

2016-07-01

Pigeon paramyxovirus type 1 (PPMV-1) is considered an antigenic and variant of avian paramyxovirus type 1 (APMV-1) that has adapted to pigeons as hosts. However, how this host-specific adaption of PPMV-1 is related to its biological characteristics is unknown. In this study, seven unique amino acids in PPMV-1 that are not present in other APMV-1 strains (n = 39 versus n = 106) were identified. R36 of the M protein was found to be not only a unique amino acid but also a positive-selection site. To investigate the role of R36 in host adaptation, a recombinant PPMV-1 with R36Q mutation was constructed. Our results indicated that the an R36Q mutation significantly attenuates pathogenicity in chickens, viral growth in both chicken embryo fibroblasts (CEFs) and pigeon embryo fibroblasts (PEFs), and virus replication and shedding in pigeons in comparison with the wild-type virus, suggesting that R36 is a key residue that evolved during the adaptation of PPMV-1 in pigeons.

[Exploring New Drug Targets through the Identification of Target Molecules of Bioactive Natural Products].

PubMed

Arai, Masayoshi

2016-01-01

With the development of cell biology and microbiology, it has become easy to culture many types of animal cells and microbes, and they are frequently used for phenotypic screening to explore medicinal seeds. On the other hand, it is recognized that cells and pathogenic microbes present in pathologic sites and infected regions of the human body display unique properties different from those under general culture conditions. We isolated several bioactive compounds from marine medicinal resources using constructed bioassay-guided separation focusing on the unique changes in the characteristics of cells and pathogenic microbes (Mycobacterium spp.) in the human body under disease conditions. In addition, we also carried out identification studies of target molecules of the bioactive compounds by methods utilizing the gene expression profile, transformants of cells or microbes, synthetic probe molecules of the isolated compounds, etc., since bioactive compounds isolated from the phenotypic screening system often target new molecules. This review presents our phenotypic screening systems, isolation of bioactive compounds from marine medicinal resources, and target identification of bioactive compounds.

Factors Associated With Time to Site Activation, Randomization, and Enrollment Performance in a Stroke Prevention Trial.

PubMed

Demaerschalk, Bart M; Brown, Robert D; Roubin, Gary S; Howard, Virginia J; Cesko, Eldina; Barrett, Kevin M; Longbottom, Mary E; Voeks, Jenifer H; Chaturvedi, Seemant; Brott, Thomas G; Lal, Brajesh K; Meschia, James F; Howard, George

2017-09-01

Multicenter clinical trials attempt to select sites that can move rapidly to randomization and enroll sufficient numbers of patients. However, there are few assessments of the success of site selection. In the CREST-2 (Carotid Revascularization and Medical Management for Asymptomatic Carotid Stenosis Trials), we assess factors associated with the time between site selection and authorization to randomize, the time between authorization to randomize and the first randomization, and the average number of randomizations per site per month. Potential factors included characteristics of the site, specialty of the principal investigator, and site type. For 147 sites, the median time between site selection to authorization to randomize was 9.9 months (interquartile range, 7.7, 12.4), and factors associated with early site activation were not identified. The median time between authorization to randomize and a randomization was 4.6 months (interquartile range, 2.6, 10.5). Sites with authorization to randomize in only the carotid endarterectomy study were slower to randomize, and other factors examined were not significantly associated with time-to-randomization. The recruitment rate was 0.26 (95% confidence interval, 0.23-0.28) patients per site per month. By univariate analysis, factors associated with faster recruitment were authorization to randomize in both trials, principal investigator specialties of interventional radiology and cardiology, pre-trial reported performance >50 carotid angioplasty and stenting procedures per year, status in the top half of recruitment in the CREST trial, and classification as a private health facility. Participation in StrokeNet was associated with slower recruitment as compared with the non-StrokeNet sites. Overall, selection of sites with high enrollment rates will likely require customization to align the sites selected to the factor under study in the trial. URL: http://www.clinicaltrials.gov. Unique identifier: NCT02089217. © 2017 American Heart Association, Inc.

Human talus bones from the Middle Pleistocene site of Sima de los Huesos (Sierra de Atapuerca, Burgos, Spain).

PubMed

Pablos, Adrián; Martínez, Ignacio; Lorenzo, Carlos; Gracia, Ana; Sala, Nohemi; Arsuaga, Juan Luis

2013-07-01

Here we present and describe comparatively 25 talus bones from the Middle Pleistocene site of the Sima de los Huesos (SH) (Sierra de Atapuerca, Burgos, Spain). These tali belong to 14 individuals (11 adult and three immature). Although variation among Middle and Late Pleistocene tali tends to be subtle, this study has identified unique morphological characteristics of the SH tali. They are vertically shorter than those of Late Pleistocene Homo sapiens, and show a shorter head and a broader lateral malleolar facet than all of the samples. Moreover, a few shared characters with Neanderthals are consistent with the hypothesis that the SH population and Neanderthals are sister groups. These shared characters are a broad lateral malleolar facet, a trochlear height intermediate between modern humans and Late Pleistocene H. sapiens, and a short middle calcaneal facet. It has been possible to propose sex assignment for the SH tali based on their size. Stature estimates based on these fossils give a mean stature of 174.4 cm for males and 161.9 cm for females, similar to that obtained based on the long bones from this same site. Copyright © 2013 Elsevier Ltd. All rights reserved.

Utilizing environmental friendly iron as a substitution element in spinel structured cathode materials for safer high energy lithium-ion batteries

DOE PAGES

Hu, Enyuan; Bak, Seong -Min; Liu, Yijin; ...

2015-12-03

Suppressing oxygen release from lithium ion battery cathodes during heating is a critical issue for the improvement of the battery safety characteristics because oxygen can exothermically react with the flammable electrolyte and cause thermal runaway. Previous studies have shown that oxygen release can be reduced by the migration of transition metal cations from octahedral sites to tetrahedral sites during heating. Such site-preferred migration is determined by the electronic structure of cations. In addition, taking advantage of the unique electronic structure of the environmental friendly Fe, this is selected as substitution element in a high energy density material LiNi 0.5Mn 1.5Omore » 4 to improve the thermal stability. The optimized LiNi 0.33Mn 1.33Fe 0.33O 4 material shows significantly improved thermal stability compared with the unsubstituted one, demonstrated by no observed oxygen release at temperatures as high as 500°C. Due to the electrochemical contribution of Fe, the high energy density feature of LiNi 0.5Mn 1.5O 4 is well preserved.« less

A landscape character assessment of three terraced areas in Campania region, Italy

NASA Astrophysics Data System (ADS)

Gravagnuolo, Antonia; Ronza, Maria; Di Martino, Ferdinando; De Rosa, Fortuna

2017-04-01

Agricultural terraces represent the territorial structure of many cultural landscapes in the Campania region, Italy. Historic urban/rural settlements and hydraulic-agrarian systems have been developed on mountains and hills, producing diverse cultural landscapes depending on the specific geological, pedological and geomorphological characteristics, which influenced the character and functions of terraces. These unique landscapes are multi-functional and provide many ecosystem services: provisioning (food, water retention, building materials); regulating and maintenance (hydrogeological stability, soil fertility, protection from soil erosion, maintenance of genetic diversity, habitat); cultural services (heritage and traditional knowledge conservation, tourism and recreation, spiritual experience, education, aesthetic quality). Three terraced landscapes in Campania are analysed, which present a rich diversity in the geological structure and formal/functional characteristics: the Roccamonfina vulcanic area, a highly fertile and lapillous soil; the Monte di Bulgheria, a clay-rich area; and finally the well-known UNESCO World Heritage site of the Amalfi Coast, a calcareous, steep rock faced area. A landscape character assessment of the three sites is processed, identifying the biophysical structure of the sites, natural systems and land use, and cultural and anthropic elements. Terraced landscapes in Campania can be regenerated, taking again an active social and economic role for the society, enhancing their multifunctionality as a key source of wellbeing. Ecosystem services are mapped and evaluated to assess benefits and costs in a multidimensional framework. Spatial analysis in GIS environment supports this process, providing a decision-support tool for mapping and assessment of terraced landscapes, to convert their actual and potential value into a resource of economic sustainable development.

Techniques for Estimating the Magnitude and Frequency of Peak Flows on Small Streams in Minnesota Based on Data through Water Year 2005

USGS Publications Warehouse

Lorenz, David L.; Sanocki, Chris A.; Kocian, Matthew J.

2010-01-01

Knowledge of the peak flow of floods of a given recurrence interval is essential for regulation and planning of water resources and for design of bridges, culverts, and dams along Minnesota's rivers and streams. Statistical techniques are needed to estimate peak flow at ungaged sites because long-term streamflow records are available at relatively few places. Because of the need to have up-to-date peak-flow frequency information in order to estimate peak flows at ungaged sites, the U.S. Geological Survey (USGS) conducted a peak-flow frequency study in cooperation with the Minnesota Department of Transportation and the Minnesota Pollution Control Agency. Estimates of peak-flow magnitudes for 1.5-, 2-, 5-, 10-, 25-, 50-, 100-, and 500-year recurrence intervals are presented for 330 streamflow-gaging stations in Minnesota and adjacent areas in Iowa and South Dakota based on data through water year 2005. The peak-flow frequency information was subsequently used in regression analyses to develop equations relating peak flows for selected recurrence intervals to various basin and climatic characteristics. Two statistically derived techniques-regional regression equation and region of influence regression-can be used to estimate peak flow on ungaged streams smaller than 3,000 square miles in Minnesota. Regional regression equations were developed for selected recurrence intervals in each of six regions in Minnesota: A (northwestern), B (north central and east central), C (northeastern), D (west central and south central), E (southwestern), and F (southeastern). The regression equations can be used to estimate peak flows at ungaged sites. The region of influence regression technique dynamically selects streamflow-gaging stations with characteristics similar to a site of interest. Thus, the region of influence regression technique allows use of a potentially unique set of gaging stations for estimating peak flow at each site of interest. Two methods of selecting streamflow-gaging stations, similarity and proximity, can be used for the region of influence regression technique. The regional regression equation technique is the preferred technique as an estimate of peak flow in all six regions for ungaged sites. The region of influence regression technique is not appropriate for regions C, E, and F because the interrelations of some characteristics of those regions do not agree with the interrelations throughout the rest of the State. Both the similarity and proximity methods for the region of influence technique can be used in the other regions (A, B, and D) to provide additional estimates of peak flow. The peak-flow-frequency estimates and basin characteristics for selected streamflow-gaging stations and regional peak-flow regression equations are included in this report.

Framework for Site Characterization for Monitored Natural Attenuation of Volatile Organic Compounds in Ground Water

EPA Science Inventory

Monitored Natural Attenuation (MNA) is unique among remedial technologies in relying entirely on natural processes to achieve site-specific objectives. Site characterization is essential to provide site-specific data and interpretations for the decision-making process (i.e., to ...

Molecular characterization of an ependymin precursor from goldfish brain.

PubMed

Königstorfer, A; Sterrer, S; Eckerskorn, C; Lottspeich, F; Schmidt, R; Hoffmann, W

1989-01-01

Ependymins are thought to be implicated in fundamental processes involved in plasticity of the goldfish CNS. Gas-phase sequencing of purified ependymins beta and gamma revealed that they share the same N-terminal sequence. Each sequence displays microheterogeneities at several positions. Based on the protein sequences obtained, we constructed synthetic oligonucleotides and used them as hybridization probes for screening cDNA libraries of goldfish brain. In this article we describe the full-length sequence of a mRNA encoding a precursor of ependymins. A cleavable signal sequence characteristic of secretory proteins is located at the N-terminal end, followed directly by the ependymin sequence. Also, two potential N-glycosylation sites were detected. A computer search revealed that ependymins form a novel family of unique proteins.

Where's the Psychology? A Commentary on "Unique Characteristics of Diagnostic Classification Models: A Comprehensive Review of the Current State-of-the-Art"

ERIC Educational Resources Information Center

Leighton, Jacqueline P.

2008-01-01

In this commentary, the author asks the analogous question, "where's the psychology?" Not because the authors of the focus article "Unique Characteristics of Diagnostic Classification Models: A Comprehensive Review of the Current State-of-the-Art" have not provided a solid review of the technical aspects of Diagnostic…

Operational Suitability Guide. Volume 2. Templates

DTIC Science & Technology

1990-05-01

Intended mission, and the required technical and operational characteristics. The mission must be adequately defined and key hardware and software ...operational availability. With the use of fault-tolerant computer hardware and software , the system R&M will significantly improve end-to-end...should Include both hardware and software elements, as appropriate. Unique characteristics or unique support concepts should be Identified if they result

Community-level characteristics associated with variation in rates of homelessness among families and single adults.

PubMed

Fargo, Jamison D; Munley, Ellen A; Byrne, Thomas H; Montgomery, Ann Elizabeth; Culhane, Dennis P

2013-12-01

We modeled rates of family and single-adult homelessness in the United States in metropolitan and nonmetropolitan regions and as a function of community-level demographic, behavioral, health, economic, and safety net characteristics. We entered community-level characteristics and US Department of Housing and Urban Development point-in-time counts for a single night in January 2009 into separate mixed-effects statistical analyses that modeled homelessness rates for 4 subpopulations: families and single adults in metropolitan and nonmetropolitan regions. Community-level factors accounted for 25% to 50% of the variance in homelessness rates across models. In metropolitan regions, alcohol consumption, social support, and several economic indicators were uniquely associated with family homelessness, and drug use and homicide were uniquely associated with single-adult homelessness. In nonmetropolitan regions, life expectancy, religious adherence, unemployment, and rent burden were uniquely associated with family homelessness, and health care access, crime, several economic indicators, and receipt of Supplemental Security Income were uniquely associated with single-adult homelessness. Considering homeless families and single adults separately enabled more precise modeling of associations between homelessness rates and community-level characteristics, indicating targets for interventions to reduce homelessness among these subpopulations.

Community-Level Characteristics Associated With Variation in Rates of Homelessness Among Families and Single Adults

PubMed Central

Fargo, Jamison D.; Munley, Ellen A.; Byrne, Thomas H.; Montgomery, Ann Elizabeth; Culhane, Dennis P.

2013-01-01

Objectives. We modeled rates of family and single-adult homelessness in the United States in metropolitan and nonmetropolitan regions and as a function of community-level demographic, behavioral, health, economic, and safety net characteristics. Methods. We entered community-level characteristics and US Department of Housing and Urban Development point-in-time counts for a single night in January 2009 into separate mixed-effects statistical analyses that modeled homelessness rates for 4 subpopulations: families and single adults in metropolitan and nonmetropolitan regions. Results. Community-level factors accounted for 25% to 50% of the variance in homelessness rates across models. In metropolitan regions, alcohol consumption, social support, and several economic indicators were uniquely associated with family homelessness, and drug use and homicide were uniquely associated with single-adult homelessness. In nonmetropolitan regions, life expectancy, religious adherence, unemployment, and rent burden were uniquely associated with family homelessness, and health care access, crime, several economic indicators, and receipt of Supplemental Security Income were uniquely associated with single-adult homelessness. Conclusions. Considering homeless families and single adults separately enabled more precise modeling of associations between homelessness rates and community-level characteristics, indicating targets for interventions to reduce homelessness among these subpopulations. PMID:24148057

New biometric modalities using internal physical characteristics

NASA Astrophysics Data System (ADS)

Mortenson, Juliana (Brooks)

2010-04-01

Biometrics is described as the science of identifying people based on physical characteristics such as their fingerprints, facial features, hand geometry, iris patterns, palm prints, or speech recognition. Notably, all of these physical characteristics are visible or detectable from the exterior of the body. These external characteristics can be lifted, photographed, copied or recorded for unauthorized access to a biometric system. Individual humans are unique internally, however, just as they are unique externally. New biometric modalities have been developed which identify people based on their unique internal characteristics. For example, "BoneprintsTM" use acoustic fields to scan the unique bone density pattern of a thumb pressed on a small acoustic sensor. Thanks to advances in piezoelectric materials the acoustic sensor can be placed in virtually any device such as a steering wheel, door handle, or keyboard. Similarly, "Imp-PrintsTM" measure the electrical impedance patterns of a hand to identify or verify a person's identity. Small impedance sensors can be easily embedded in devices such as smart cards, handles, or wall mounts. These internal biometric modalities rely on physical characteristics which are not visible or photographable, providing an added level of security. In addition, both the acoustic and impedance methods can be combined with physiologic measurements such as acoustic Doppler or impedance plethysmography, respectively. Added verification that the biometric pattern came from a living person can be obtained. These new biometric modalities have the potential to allay user concerns over protection of privacy, while providing a higher level of security.*

Ambient concentrations and insights on organic and elemental carbon dynamics in São Paulo, Brazil

NASA Astrophysics Data System (ADS)

Monteiro dos Santos, Djacinto A.; Brito, Joel F.; Godoy, José Marcus; Artaxo, Paulo

2016-11-01

The São Paulo Metropolitan Area (SPMA) is a megacity with about 20 million people and about 8 million vehicles, most of which are fueled with a significant fraction of ethanol - making it a unique case worldwide. This study presents organic and elemental carbon measurements using thermal-optical analysis from quartz filters collected in four sampling sites within the SPMA. Overall Organic Carbon (OC) concentration was comparable at all sites, where Street Canyon had the highest concentration (3.37 μg m-3) and Park site the lowest (2.65 μg m-3). Elemental Carbon (EC), emitted as result of incomplete combustion, has been significantly higher at the Street Canyon site (6.11 μg m-3) in contrast to all other three sites, ranging from 2.25 μg m-3 (Downtown) to 1.50 μg m-3 (Park). For all sampling sites, the average OC:EC ratio are found on the lower bound (<2) of what is usually observed for other megacities, highlighting the significant contribution of EC in Sao Paulo. At the Street Canyon site, average OC:EC ratio was 0.56, to our knowledge the lowest value ever observed for any urban site. An approach for apportionment between primary and secondary organic carbon based on primary OC:EC ratio was evaluated. The secondary OC was estimated to be 30-40% of total OC concentrations throughout the various sampling sites. The organic carbon dynamics has been further studied using each of the thermally-derived organic carbon fractions. Each of these has been studied regarding their correlation with EC and the correlation between different sites. The analyses have identified that the OC3 and OC4, i.e., the carbon fraction which evolves from the filter at temperatures above 450 °C, presents a regional behavior, with high correlation among all sites. Conversely, OC1, the first fraction to evolve, has depicted a more local characteristic. Furthermore, the fraction of OC which becomes char during the temperature increase under inert atmosphere (the Pyrolytic Carbon-PC) has been the only fraction not to present a significant correlation with EC. Since that EC is assumed to be a primary emission marker, it indicates that PC is not significant in traffic emissions. This study provided innovative insights of the organic aerosol content associated with air pollution dynamics in a megacity impacted by a unique vehicular fleet. It also shows the need of implementation of EURO VI technology and to improve mass transport systems such a metro and more bus corridors to allow better transport for 19 million people in the SPMA.

SUMMARY OF TECHNIQUES AND UNIQUE USES FOR DIRECT PUSH METHODS IN SITE CHARACTERIZATION ON CONTAMINATED FIELD SITES

EPA Science Inventory

Site characterization of subsurface contaminant transport is often hampered by a lack of knowledge of site heterogeneity and temporal variations in hydrogeochemistry. Two case studies are reviewed to illustrate the utility of macro-scale mapping information along with spatially-...

Comparison of biological and genomic characteristics between a newly isolated mink enteritis parvovirus MEV-LHV and an attenuated strain MEV-L.

PubMed

Mao, Yaping; Wang, Jigui; Hou, Qiang; Xi, Ji; Zhang, Xiaomei; Bian, Dawei; Yu, Yongle; Wang, Xi; Liu, Weiquan

2016-06-01

A virus isolated from mink showing clinical signs of enteritis was identified as a high virulent mink enteritis parvovirus (MEV) based on its biological characteristics in vivo and in vitro. Mink, challenged with this strain named MEV-LHV, exhibited severe pathological lesions as compared to those challenged with attenuated strain MEV-L. MEV-LHV also showed higher infection and replication efficiencies in vitro than MEV-L. Sequence of the complete genome of MEV-LHV was determined and analyzed in comparison with those in GenBank, which revealed that MEV-LHV shared high homology with virulent strain MEV SD12/01, whereas MEV-L was closely related to Abashiri and vaccine strain MEVB, and belonged to a different branch of the phylogenetic tree. The genomes of the two strains differed by insertions and deletions in their palindromic termini and specific unique mutations (especially VP2 300) in coding sequences which may be involved in viral replication and pathogenicity. The results of this study provide a better understanding of the biological and genomic characteristics of MEV and identify certain regions and sites that may be involved in viral replication and pathogenicity.

Ub-ISAP: a streamlined UNIX pipeline for mining unique viral vector integration sites from next generation sequencing data.

PubMed

Kamboj, Atul; Hallwirth, Claus V; Alexander, Ian E; McCowage, Geoffrey B; Kramer, Belinda

2017-06-17

The analysis of viral vector genomic integration sites is an important component in assessing the safety and efficiency of patient treatment using gene therapy. Alongside this clinical application, integration site identification is a key step in the genetic mapping of viral elements in mutagenesis screens that aim to elucidate gene function. We have developed a UNIX-based vector integration site analysis pipeline (Ub-ISAP) that utilises a UNIX-based workflow for automated integration site identification and annotation of both single and paired-end sequencing reads. Reads that contain viral sequences of interest are selected and aligned to the host genome, and unique integration sites are then classified as transcription start site-proximal, intragenic or intergenic. Ub-ISAP provides a reliable and efficient pipeline to generate large datasets for assessing the safety and efficiency of integrating vectors in clinical settings, with broader applications in cancer research. Ub-ISAP is available as an open source software package at https://sourceforge.net/projects/ub-isap/ .

HIV integration sites in latently infected cell lines: evidence of ongoing replication.

PubMed

Symons, Jori; Chopra, Abha; Malatinkova, Eva; De Spiegelaere, Ward; Leary, Shay; Cooper, Don; Abana, Chike O; Rhodes, Ajantha; Rezaei, Simin D; Vandekerckhove, Linos; Mallal, Simon; Lewin, Sharon R; Cameron, Paul U

2017-01-13

Assessing the location and frequency of HIV integration sites in latently infected cells can potentially inform our understanding of how HIV persists during combination antiretroviral therapy. We developed a novel high throughput sequencing method to evaluate HIV integration sites in latently infected cell lines to determine whether there was virus replication or clonal expansion in these cell lines observed as multiple integration events at the same position. We modified a previously reported method using random DNA shearing and PCR to allow for high throughput robotic processing to identify the site and frequency of HIV integration in latently infected cell lines. Latently infected cell lines infected with intact virus demonstrated multiple distinct HIV integration sites (28 different sites in U1, 110 in ACH-2 and 117 in J1.1 per 150,000 cells). In contrast, cell lines infected with replication-incompetent viruses (J-Lat cells) demonstrated single integration sites. Following in vitro passaging of the ACH-2 cell line, we observed a significant increase in the frequency of unique HIV integration sites and there were multiple mutations and large deletions in the proviral DNA. When the ACH-2 cell line was cultured with the integrase inhibitor raltegravir, there was a significant decrease in the number of unique HIV integration sites and a transient increase in the frequency of 2-LTR circles consistent with virus replication in these cells. Cell lines latently infected with intact HIV demonstrated multiple unique HIV integration sites indicating that these cell lines are not clonal and in the ACH-2 cell line there was evidence of low level virus replication. These findings have implications for the use of latently infected cell lines as models of HIV latency and for the use of these cells as standards.

Anthropogenic plutonium-244 in the environment: Insights into plutonium’s longest-lived isotope

DOE PAGES

Armstrong, Christopher R.; Brant, Heather A.; Nuessle, Patterson R.; ...

2016-02-22

Owing to the rich history of heavy element production in the unique high flux reactors that operated at the Savannah River Site, USA (SRS) decades ago, trace quantities of plutonium with highly unique isotopic characteristics still persist today in the SRS terrestrial environment. Development of an effective sampling, processing, and analysis strategy enables detailed monitoring of the SRS environment, revealing plutonium isotopic compositions, e.g., 244Pu, that reflect the unique legacy of plutonium production at SRS. This work describes the first long-term investigation of anthropogenic 244Pu occurrence in the environment. Environmental samples, consisting of collected foot borne debris, were taken atmore » SRS over an eleven year period, from 2003 to 2014. Separation and purification of trace plutonium was carried out followed by three stage thermal ionization mass spectrometry (3STIMS) measurements for plutonium isotopic content and isotopic ratios. Furthermore, significant 244Pu was measured in all of the years sampled with the highest amount observed in 2003. The 244Pu content, in femtograms (fg = 10 –15 g) per gram, ranged from 0.31 fg/g to 44 fg/g in years 2006 and 2003 respectively. In all years, the 244Pu/ 239Pu atom ratios were significantly higher than global fallout, ranging from 0.003 to 0.698 in years 2014 and 2003 respectively.« less

Anthropogenic plutonium-244 in the environment: Insights into plutonium’s longest-lived isotope

PubMed Central

Armstrong, Christopher R.; Brant, Heather A.; Nuessle, Patterson R.; Hall, Gregory; Cadieux, James R.

2016-01-01

Owing to the rich history of heavy element production in the unique high flux reactors that operated at the Savannah River Site, USA (SRS) decades ago, trace quantities of plutonium with highly unique isotopic characteristics still persist today in the SRS terrestrial environment. Development of an effective sampling, processing, and analysis strategy enables detailed monitoring of the SRS environment, revealing plutonium isotopic compositions, e.g., 244Pu, that reflect the unique legacy of plutonium production at SRS. This work describes the first long-term investigation of anthropogenic 244Pu occurrence in the environment. Environmental samples, consisting of collected foot borne debris, were taken at SRS over an eleven year period, from 2003 to 2014. Separation and purification of trace plutonium was carried out followed by three stage thermal ionization mass spectrometry (3STIMS) measurements for plutonium isotopic content and isotopic ratios. Significant 244Pu was measured in all of the years sampled with the highest amount observed in 2003. The 244Pu content, in femtograms (fg = 10−15 g) per gram, ranged from 0.31 fg/g to 44 fg/g in years 2006 and 2003 respectively. In all years, the 244Pu/239Pu atom ratios were significantly higher than global fallout, ranging from 0.003 to 0.698 in years 2014 and 2003 respectively. PMID:26898531

Differential DNA methylation and lymphocyte proportions in a Costa Rican high longevity region.

PubMed

McEwen, Lisa M; Morin, Alexander M; Edgar, Rachel D; MacIsaac, Julia L; Jones, Meaghan J; Dow, William H; Rosero-Bixby, Luis; Kobor, Michael S; Rehkopf, David H

2017-01-01

The Nicoya Peninsula in Costa Rica has one of the highest old-age life expectancies in the world, but the underlying biological mechanisms of this longevity are not well understood. As DNA methylation is hypothesized to be a component of biological aging, we focused on this malleable epigenetic mark to determine its association with current residence in Nicoya versus elsewhere in Costa Rica. Examining a population's unique DNA methylation pattern allows us to differentiate hallmarks of longevity from individual stochastic variation. These differences may be characteristic of a combination of social, biological, and environmental contexts. In a cross-sectional subsample of the Costa Rican Longevity and Healthy Aging Study, we compared whole blood DNA methylation profiles of residents from Nicoya ( n  = 48) and non-Nicoya (other Costa Rican regions, n  = 47) using the Infinium HumanMethylation450 microarray. We observed a number of differences that may be markers of delayed aging, such as bioinformatically derived differential CD8+ T cell proportions. Additionally, both site- and region-specific analyses revealed DNA methylation patterns unique to Nicoyans. We also observed lower overall variability in DNA methylation in the Nicoyan population, another hallmark of younger biological age. Nicoyans represent an interesting group of individuals who may possess unique immune cell proportions as well as distinct differences in their epigenome, at the level of DNA methylation.

Concentration and mindfulness meditations: unique forms of consciousness?

PubMed

Dunn, B R; Hartigan, J A; Mikulas, W L

1999-09-01

Electroencephalographic (EEG) recordings from 19 scalp recording sites were used to differentiate among two posited unique forms of mediation, concentration and mindfulness, and a normal relaxation control condition. Analyzes of all traditional frequency bandwidth data (i.e., delta 1-3 Hz; theta, 4-7 Hz; alpha, 8-12 Hz; beta 1, 13-25 Hz; beta 2, 26-32 Hz) showed strong mean amplitude frequency differences between the two meditation conditions and relaxation over numerous cortical sites. Furthermore, significant differences were obtained between concentration and mindfulness states at all bandwidths. Taken together, our results suggest that concentration and mindfulness "meditations" may be unique forms of consciousness and are not merely degrees of a state of relaxation.

Evaluation of soil contamination by explosives and metals at the Land Force Central Area Training Centre (LFCA TC) Meaford, Ontario (Phase I)

DTIC Science & Technology

2009-05-01

found in ammunition. The site characterization allowed the development of a unique expertise and positioned our departments to better understand the...environnementaux des composés énergétiques que l’on retrouve dans les munitions. La caractérisation des sites a permis de développer une expertise unique et a...éventualités pour prendre des mesures correctives, si nécessaire. Les premiers sites d’entraînement à être évalués ont été des bases de l’armée, telles que

On the quantitative inventory of the riverscape

USGS Publications Warehouse

Leopold, Luna Bergere; O'Brien Marchand, Maura

1968-01-01

In the vicinity of Berkeley, California, 24 minor valleys were described in terms of factors chosen to represent aspects of the river landscape. A total of 28 factors were evaluated at each site. Some were directly measurable, others were estimated, but each observation was assigned to one of five categories for that factor. Each factor for each site was then expressed as a uniqueness ratio, which depended on the number of sites being in the same category. The uniqueness ratio is believed to represent one way the scarcity of a given riverscape can be ranked quantitatively without bias based on notions of good or bad, and without assigning monetary value.

Subcortical electrostimulation to identify network subserving motor control.

PubMed

Schucht, Philippe; Moritz-Gasser, Sylvie; Herbet, Guillaume; Raabe, Andreas; Duffau, Hugues

2013-11-01

Recent anatomical-functional studies have transformed our understanding of cerebral motor control away from a hierarchical structure and toward parallel and interconnected specialized circuits. Subcortical electrical stimulation during awake surgery provides a unique opportunity to identify white matter tracts involved in motor control. For the first time, this study reports the findings on motor modulatory responses evoked by subcortical stimulation and investigates the cortico-subcortical connectivity of cerebral motor control. Twenty-one selected patients were operated while awake for frontal, insular, and parietal diffuse low-grade gliomas. Subcortical electrostimulation mapping was used to search for interference with voluntary movements. The corresponding stimulation sites were localized on brain schemas using the anterior and posterior commissures method. Subcortical negative motor responses were evoked in 20/21 patients, whereas acceleration of voluntary movements and positive motor responses were observed in three and five patients, respectively. The majority of the stimulation sites were detected rostral of the corticospinal tract near the vertical anterior-commissural line, and additional sites were seen in the frontal and parietal white matter. The diverse interferences with motor function resulting in inhibition and acceleration imply a modulatory influence of the detected fiber network. The subcortical stimulation sites were distributed veil-like, anterior to the primary motor fibers, suggesting descending pathways originating from premotor areas known for negative motor response characteristics. Further stimulation sites in the parietal white matter as well as in the anterior arm of the internal capsule indicate a large-scale fronto-parietal motor control network. Copyright © 2012 Wiley Periodicals, Inc.

Kinemage of action - Proposed reaction mechanism of glutamate-1-semialdehyde aminomutase at an atomic level

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sorensen, John L., E-mail: John_Sorensen@umanitoba.ca; Stetefeld, Joerg, E-mail: stetefel@cc.umanitoba.ca

2011-10-07

Highlights: {yields} Inhibitors of tetrapyrrole cofactor biosynthesis may be useful antibiotics. {yields} Mechanism of critical enzyme, glutamate-1-semialdehyde aminomutase, is presented. {yields} Unique vitamin B6-dependant enzyme traps intermediate in active site. {yields} Molecular dynamics show that a re-orientation of the substrate is required. -- Abstract: Glutamate-1-semialdehyde aminomutase (GSAM), a key enzyme in tetrapyrrole cofactor biosynthesis, performs a unique transamination on a single substrate. The substrate, glutamate-1-semialdehyde (GSA), undergoes a reaction that exchanges the position of an amine and a carbonyl group to produce 5-aminolevulinic acid (ALA). This transamination reaction is unique in the fact that is does not require an externalmore » cofactor to act as a nitrogen donor or acceptor in this transamination reaction. One of the other remarkable features of the catalytic mechanism is the release free in the enzyme active site of the intermediate 4,5-diaminovaleric acid (DAVA). The action of a gating loop prevents the escape of DAVA from the active site. In a MD simulation approach, using snapshots provided by X-ray crystallography and protein crystal absorption spectrometry data, the individual catalytic steps in this unique intramolecular transamination have been elucidated.« less

Identification and characterization of (/sup 3/H)-rauwolscine binding to alpha2-adrenoceptors in the canine saphenous vein

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gout, B.

1988-01-01

The biochemical exploration of the alpha2-adrenergic receptors was investigated in the canine saphenous vein using the highly selective alpha2-adrenergic antagonist rauwolscine as a tritiated ligand. Following an enzymatic digestive pretreatment, the authors isolated a purified smooth muscle cell membranes fraction from saphenous veins in quantity sufficient to permit them to study the venous alpha2-adrenoreceptor content. The binding of tritiated rauwolscine was rapid, specific, saturable and reversible. The presence of high affinity sites with a density of binding Bmax of 125.2 /+ -/ 43.1 fmol/mg protein was demonstrated on a unique class of non interacting sites. The kinetically derived Kd wasmore » 1.28 nM, in good agreement with the value obtained from saturation isotherms. The pharmacological profile of these sites was assessed by the comparison of the potency of alpha-adrenergic agonists and antagonists to inhibit 1 nM (/sup 3/H)-rauwolscine. Their efficacy was respectively: rauwolscine > phentolamine > RX 781094 > clonidine >> prazosin > (-)-phenylephrine > (-)-noradrenaline. The results showed that (/sup 3/H)-rauwolscine bound specifically to sites in their membranal preparation, which had the pharmacological characteristics of the alpha2-adrenoceptors. The correlation between biochemical and pharmacological data revealed the usefulness of binding methods in the further study of adrenergic mechanisms in the canine saphenous vein.« less

Identification of one of the apurinic/apyrimidinic lyase active sites of topoisomerase V by structural and functional studies

PubMed Central

Rajan, Rakhi; Prasad, Rajendra; Taneja, Bhupesh; Wilson, Samuel H.; Mondragón, Alfonso

2013-01-01

Topoisomerase V (Topo-V) is the only member of a novel topoisomerase subtype. Topo-V is unique because it is a bifunctional enzyme carrying both topoisomerase and DNA repair lyase activities within the same protein. Previous studies had shown that the topoisomerase domain spans the N-terminus of the protein and is followed by 12 tandem helix–hairpin–helix [(HhH)2] domains. There are at least two DNA repair lyase active sites for apurinic/apyrimidinic (AP) site processing, one within the N-terminal region and the second within the C-terminal domain of Topo-V, but their exact locations and characteristics are unknown. In the present study, the N-terminal 78-kDa fragment of Topo-V (Topo-78), containing the topoisomerase domain and one of the lyase DNA repair domains, was characterized by structural and biochemical studies. The results show that an N-terminal 69-kDa fragment is the minimal fragment with both topoisomerase and AP lyase activities. The lyase active site of Topo-78 is at the junction of the fifth and sixth (HhH)2 domains. From the biochemical and structural data, it appears that Lys571 is the most probable nucleophile responsible for the lyase activity. Our experiments also suggest that Topo-V most likely acts as a Class I AP endonuclease in vivo. PMID:23125368

NACP Synthesis: Evaluating modeled carbon state and flux variables against multiple observational constraints (Invited)

NASA Astrophysics Data System (ADS)

Thornton, P. E.; Nacp Site Synthesis Participants

2010-12-01

The North American Carbon Program (NACP) synthesis effort includes an extensive intercomparison of modeled and observed ecosystem states and fluxes preformed with multiple models across multiple sites. The participating models span a range of complexity and intended application, while the participating sites cover a broad range of natural and managed ecosystems in North America, from the subtropics to arctic tundra, and coastal to interior climates. A unique characteristic of this collaborative effort is that multiple independent observations are available at all sites: fluxes are measured with the eddy covariance technique, and standard biometric and field sampling methods provide estimates of standing stock and annual production in multiple categories. In addition, multiple modeling approaches are employed to make predictions at each site, varying, for example, in the use of diagnostic vs. prognostic leaf area index. Given multiple independent observational constraints and multiple classes of model, we evaluate the internal consistency of observations at each site, and use this information to extend previously derived estimates of uncertainty in the flux observations. Model results are then compared with all available observations and models are ranked according to their consistency with each type of observation (high frequency flux measurement, carbon stock, annual production). We demonstrate a range of internal consistency across the sites, and show that some models which perform well against one observational metric perform poorly against others. We use this analysis to construct a hypothesis for combining eddy covariance, biometrics, and other standard physiological and ecological measurements which, as data collection proceeded over several years, would present an increasingly challenging target for next generation models.

Characteristics of the original patient navigation programs to reduce disparities in the diagnosis and treatment of breast cancer.

PubMed

Vargas, Roberto B; Ryan, Gery W; Jackson, Catherine A; Rodriguez, Rian; Freeman, Harold P

2008-07-15

Patient navigation is an intervention developed to reduce disparities in cancer care that is being widely replicated and receiving considerable support for demonstration projects and research to test its effectiveness. In the current study, the authors present an in-depth descriptive analysis of the original patient navigation programs to inform current and future program development. A qualitative multistakeholder case study using interviews and site visits of the first patient navigation site and 2 sites subsequently developed by the leadership of the original site were evaluated. At these sites, patient navigation is a system, as opposed to a person, comprised primarily of navigators and directors that work together to remove barriers and facilitate access in a well-defined course of care; navigators were from the community or were culturally similar to the patient population served but were also paid employees of the clinical care site with detailed knowledge of the clinical course patients must traverse to complete care plans. Directors had administrative authority over the clinical facility and social capital across institutions, and communicated regularly and openly with navigators to implement system level changes to remove barriers to care. Contextual factors such as policies supporting breast cancer care also influenced the implementation of these programs. The first patient navigation programs combined community and culturally sensitive care-coordination with aspects of disease management programs to reduce racial, ethnic, and poverty-driven disparities in care. Future efforts to replicate and evaluate patient navigation should take into account these unique aspects of the original patient navigation programs. (c) 2008 American Cancer Society.

Root Water Uptake and Soil Moisture Pattern Dynamics - Capturing Connections, Controls and Causalities

NASA Astrophysics Data System (ADS)

Blume, T.; Heidbuechel, I.; Hassler, S. K.; Simard, S.; Guntner, A.; Stewart, R. D.; Weiler, M.

2015-12-01

We hypothesize that there is a shift in controls on landscape scale soil moisture patterns when plants become active during the growing season. Especially during the summer soil moisture patterns are not only controlled by soils, topography and related abiotic site characteristics but also by root water uptake. Root water uptake influences soil moisture patterns both in the lateral and vertical direction. Plant water uptake from different soil depths is estimated based on diurnal fluctuations in soil moisture content and was investigated with a unique setup of 46 field sites in Luxemburg and 15 field sites in Germany. These sites cover a range of geologies, soils, topographic positions and types of vegetation. Vegetation types include pasture, pine forest (young and old) and different deciduous forest stands. Available data at all sites includes information at high temporal resolution from 3-5 soil moisture and soil temperature profiles, matrix potential, piezometers and sapflow sensors as well as standard climate data. At sites with access to a stream, discharge or water level is also recorded. The analysis of soil moisture patterns over time indicates a shift in regime depending on season. Depth profiles of root water uptake show strong differences between different forest stands, with maximum depths ranging between 50 and 200 cm. Temporal dynamics of signal strength within the profile furthermore suggest a locally shifting spatial distribution of root water uptake depending on water availability. We will investigate temporal thresholds (under which conditions spatial patterns of root water uptake become most distinct) as well as landscape controls on soil moisture and root water uptake dynamics.

CLIP-seq analysis of multi-mapped reads discovers novel functional RNA regulatory sites in the human transcriptome.

PubMed

Zhang, Zijun; Xing, Yi

2017-09-19

Crosslinking or RNA immunoprecipitation followed by sequencing (CLIP-seq or RIP-seq) allows transcriptome-wide discovery of RNA regulatory sites. As CLIP-seq/RIP-seq reads are short, existing computational tools focus on uniquely mapped reads, while reads mapped to multiple loci are discarded. We present CLAM (CLIP-seq Analysis of Multi-mapped reads). CLAM uses an expectation-maximization algorithm to assign multi-mapped reads and calls peaks combining uniquely and multi-mapped reads. To demonstrate the utility of CLAM, we applied it to a wide range of public CLIP-seq/RIP-seq datasets involving numerous splicing factors, microRNAs and m6A RNA methylation. CLAM recovered a large number of novel RNA regulatory sites inaccessible by uniquely mapped reads. The functional significance of these sites was demonstrated by consensus motif patterns and association with alternative splicing (splicing factors), transcript abundance (AGO2) and mRNA half-life (m6A). CLAM provides a useful tool to discover novel protein-RNA interactions and RNA modification sites from CLIP-seq and RIP-seq data, and reveals the significant contribution of repetitive elements to the RNA regulatory landscape of the human transcriptome. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Thyroid Disease and Surgery in CHEER: The Nation’s Otolaryngology-Head and Neck Surgery Practice Based Network

PubMed Central

Parham, Kourosh; Chapurin, Nikita; Schulz, Kris; Shin, Jennifer J.; Pynnonen, Melissa A.; Witsell, David L.; Langman, Alan; Nguyen-Huynh, Anh; Ryan, Sheila E.; Vambutas, Andrea; Wolfley, Anne; Roberts, Rhonda; Lee, Walter T.

2017-01-01

Objectives 1) Describe thyroid-related diagnoses and procedures in CHEER across academic and community sites. 2) Compare management of malignant thyroid disease across these sites, and 3) Provide practice based data related to flexible laryngoscopy vocal fold assessment before and after thyroid surgery based on AAO-HNSF Clinical Practice Guidelines. Study Design Review of retrospective data collection (RDC) database of the CHEER network using ICD-9 and CPT codes related to thyroid conditions. Setting Multisite practice based network. Subjects and Methods There were 3,807 thyroid patients (1,392 malignant; 2,415 benign) with 10,160 unique visits identified from 1 year of patient data in the RDC. Analysis was performed for identified cohort of patients using demographics, site characteristics and diagnostic and procedural distribution. Results Mean number of patients with thyroid disease per site was 238 (range 23–715). In community practices, 19% of patients with thyroid disease had cancer versus 45% in the academic setting (p<0.001). While academic sites manage more cancer patients, community sites are also surgically treating thyroid cancer, and performed more procedures per cancer patient (4.2 vs. 3.5, p<0.001). Vocal fold function was assessed by flexible laryngoscopy in 34.0% of pre-operative patients and in 3.7% post-operatively. Conclusion This is the first overview of malignant and benign thyroid disease through CHEER. It shows how the RDC can be used alone and with national guidelines to inform of clinical practice patterns in academic and community sites. This demonstrates the potential for future thyroid related studies utilizing the Otolaryngology-H&N Surgery’s practice-based research network. PMID:27371622

The earliest long-distance obsidian transport: Evidence from the ∼200 ka Middle Stone Age Sibilo School Road Site, Baringo, Kenya.

PubMed

Blegen, Nick

2017-02-01

This study presents the earliest evidence of long-distance obsidian transport at the ∼200 ka Sibilo School Road Site (SSRS), an early Middle Stone Age site in the Kapthurin Formation, Kenya. The later Middle Pleistocene of East Africa (130-400 ka) spans significant and interrelated behavioral and biological changes in human evolution including the first appearance of Homo sapiens. Despite the importance of the later Middle Pleistocene, there are relatively few archaeological sites in well-dated contexts (n < 10) that document hominin behavior from this time period. In particular, geochemically informed evidence of long-distance obsidian transport, important for investigating expansion of intergroup interactions in hominin evolution, is rare from the Middle Pleistocene record of Africa. The SSRS offers a unique contribution to this small but growing dataset. Tephrostratigraphic analysis of tuffs encasing the SSRS provides a minimum age of ∼200 ka for the site. Levallois points and methods of core preparation demonstrate characteristic Middle Stone Age lithic technologies present at the SSRS. A significant portion (43%) of the lithic assemblage is obsidian. The SSRS obsidian comes from three different sources located at distances of 25 km, 140 km and 166 km from the site. The majority of obsidian derives from the farthest source, 166 km to the south of the site. The SSRS thus provides important new evidence that long-distance raw material transport, and the expansion of hominin intergroup interactions that this entails, was a significant feature of hominin behavior ∼200 ka, the time of the first appearance of H. sapiens, and ∼150,000 years before similar behaviors were previously documented in the region. Copyright © 2016 Elsevier Ltd. All rights reserved.

Calcareous forest seepages acting as biodiversity hotspots and refugia for woodland snail faunas

NASA Astrophysics Data System (ADS)

Horsák, Michal; Tajovská, Eva; Horsáková, Veronika

2017-07-01

Land-snail species richness has repeatedly been found to increase with the increasing site calcium content and humidity. These two factors, reported as the main drivers of land-snail assemblage diversity, are also among the main habitat characteristics of calcareous seepages. Here we explore local species richness and compositional variation of forest spring-fed patches (i.e. seepages), to test the hypothesis that these habitats might act as biodiversity hotspots and refugia of regional snail faunas. In contrast to treeless spring fens, only little is known about land snail faunas inhabiting forest seepages. Studying 25 isolated calcareous forest seepages, evenly distributed across the White Carpathians Protected Landscape Area (SE Czech Republic), we found that these sites, albeit spatially very limited, can harbour up to 66% of the shelled land-snail species known to occur in this well-explored protected area (in total 83 species). By comparing land snail assemblages of the studied seepages with those occurring in the woodland surroundings of each site as well as those previously sampled in 28 preserved forest sites within the study area, we found the seepages to be among the most species rich sites. Although the numbers of species did not statistically differ among these three systems, we found highly significant differences in species composition. Seepage faunas were composed of many species significantly associated with spring sites, in contrast to the assemblages of both surrounding and preserved forest sites. Our results highly support the hypothesis that calcareous forest seepages might serve as refugia and biodiversity hotspots of regional land snail faunas. Protection of these unique habitats challenges both conservation plans and forest management guidelines as they might act as sources for the recolonization and restoration of forest snail assemblages particularly in areas impoverished by harvesting and clearcutting.

Driver speed selection on high-speed two-lane highways: Comparing speed profiles between uniform and differential speed limits.

PubMed

Russo, Brendan J; Savolainen, Peter T; Gates, Timothy J; Kay, Jonathan J; Frazier, Sterling

2017-07-04

Although a considerable amount of prior research has investigated the impacts of speed limits on traffic safety and operations, much of this research, and nearly all of the research related to differential speed limits, has been specific to limited access freeways. The unique safety and operational issues on highways without access control create difficulty relating the conclusions from prior freeway-related speed limit research to 2-lane highways, particularly research on differential limits due to passing limitations and subsequent queuing. Therefore, the objective of this study was to assess differences in driver speed selection with respect to the posted speed limit on rural 2-lane highways, with a particular emphasis on the differences between uniform and differential speed limits. Data were collected from nearly 59,000 vehicles across 320 sites in Montana and 4 neighboring states. Differences in mean speeds, 85th percentile speeds, and the standard deviation in speeds for free-flowing vehicles were examined across these sites using ordinary least squares regression models. Ultimately, the results of the analysis show that the mean speed, 85th percentile speed, and variability in travel speeds for free-flowing vehicles on 2-lane highways are generally lower at locations with uniform 65 mph speed limits, compared to locations with differential limits of 70 mph for cars and 60 mph for trucks. In addition to posted speed limits, several site characteristics were shown to influence speed selection including shoulder widths, frequency of horizontal curves, percentage of the segment that included no passing zones, and hourly volumes. Differences in vehicle speed characteristics were also observed between states, indicating that speed selection may also be influenced by local factors, such as driver population or enforcement.

Marine biology, intertidal ecology, and a new place for biology.

PubMed

Benson, Keith R

2015-01-01

At the present time, there is considerable interest for the physical setting of science, that is, its actual 'place' of practice. Among historians of biology, place has been considered to be a crucial component for the study of ecology. Other historians have noted the 'built' environments (laboratories) for the study of biology along the seashore, even referring to these places in terms more applicable to vacation sites. In this paper, I examine the place of intertidal ecology investigations, both in terms of the physical space and the built space. Part of the examination will investigate the aesthetic aspect of the Pacific Coast, part will evaluate the unique character of the intertidal zone, and part will consider the construction of natural laboratories and built laboratories as characteristic places for biology.

Humans thrived in South Africa through the Toba eruption about 74,000 years ago

NASA Astrophysics Data System (ADS)

Smith, Eugene I.; Jacobs, Zenobia; Johnsen, Racheal; Ren, Minghua; Fisher, Erich C.; Oestmo, Simen; Wilkins, Jayne; Harris, Jacob A.; Karkanas, Panagiotis; Fitch, Shelby; Ciravolo, Amber; Keenan, Deborah; Cleghorn, Naomi; Lane, Christine S.; Matthews, Thalassa; Marean, Curtis W.

2018-03-01

Approximately 74 thousand years ago (ka), the Toba caldera erupted in Sumatra. Since the magnitude of this eruption was first established, its effects on climate, environment and humans have been debated. Here we describe the discovery of microscopic glass shards characteristic of the Youngest Toba Tuff—ashfall from the Toba eruption—in two archaeological sites on the south coast of South Africa, a region in which there is evidence for early human behavioural complexity. An independently derived dating model supports a date of approximately 74 ka for the sediments containing the Youngest Toba Tuff glass shards. By defining the input of shards at both sites, which are located nine kilometres apart, we are able to establish a close temporal correlation between them. Our high-resolution excavation and sampling technique enable exact comparisons between the input of Youngest Toba Tuff glass shards and the evidence for human occupation. Humans in this region thrived through the Toba event and the ensuing full glacial conditions, perhaps as a combined result of the uniquely rich resource base of the region and fully evolved modern human adaptation.

Fluorescent components and spatial patterns of chromophoric dissolved organic matters in Lake Taihu, a large shallow eutrophic lake in China.

PubMed

Yao, Bo; Hu, Chunming; Liu, Qingquan

2016-11-01

Water samples at both surface and bottom layers were taken from 102 sites in Lake Taihu to study the fluorescent components and spatial patterns of chromophoric dissolved organic matters (CDOM). Three-dimensional excitation-emission matrix data obtained from the samples were analyzed by parallel factor approach in which four humic-like and two protein-like fluorescent components (named C1-C6) were identified. The results showed that fluorescence intensities were higher in the northern and western lake regions, and notable declines of fluorescence maxima (F max ) were observed from the northwest to the center and then to the southeast of the lake. Calculated biological index (BIX) values ranged from 0.88 to 1.44 and humification index (HIX) values from 0.64 to 3.37 for all the samples. The spatial variations of BIX and HIX values suggested stronger allochthonous CDOM characteristics in Zhushan Bay and the western area and autochthonous characteristics in the southern and eastern areas. Vertically, the average F max value of the surface samples was about 6 % less than that of the bottom samples, but noticeable variations existed among different sampling sites and components. These distribution characteristics of CDOM were mainly attributed to the spatial heterogeneity of sources and wind-induced transportation process. Interestingly, the C6 component (Ex max /Em max  = 250/455 nm) seemed to be unique in samples from Zhushan Bay and probably resulted from the discharge of the Taige River. Therefore, it could be used as an indicator of point-source discharge and a tracer to study the fate of CDOM in the lake.

Effective and less invasive diagnostic strategy for gastrointestinal GVHD

PubMed Central

Endo, Katsuya; Fujishima, Fumiyoshi; Kuroha, Masatake; Moroi, Rintaro; Onodera, Motoyuki; Naito, Takeo; Kanazawa, Yoshitake; Kimura, Tomoya; Shiga, Hisashi; Kakuta, Yoichi; Kinouchi, Yoshitaka; Shimosegawa, Tooru

2018-01-01

Background and study aims  Rectosigmoidoscopy with biopsy has been regarded to be a useful procedure to diagnose gastrointestinal graft-versus-host disease (GVHD). However, little is known about the specific colonoscopic features of gastrointestinal GVHD. In this study, we focused on the 4 unique colonoscopic findings – orange peel appearance, spotty redness, small mucosal sloughing, and diffuse mucosal defect – which are possible specific findings of gastrointestinal GVHD. We aimed to estimate the usefulness of these four unique colonoscopic findings in the rectosigmoid portion to diagnose gastrointestinal GVHD. Patients and methods  Seventy patients who were histologically diagnosed with gastrointestinal GVHD at our institute were retrospectively enrolled. Colonoscopic findings were reviewed, focusing on the four characteristic findings. The percentage of the positive cases for the characteristic findings was calculated. The final scoping portion and the number of cases showing any of the four characteristic findings in the rectosigmoid portion were also evaluated. The relationships between biopsy sites and the histological findings were also evaluated. Results  Orange peel appearance was observed in 66 cases (94.3 %). Spotty redness was observed in 45 cases (64.3 %). Small mucosal sloughing was observed in 49 cases (70.0 %). Diffuse mucosal defect was observed in six cases (8.6 %). The number of cases that were concurrently positive for one, two, and three findings were 16 (20.8 %), 20 (26.0 %), and 34 (48.6 %), respectively. Fifty-eight cases (82.9 %) were investigated up to the rectosigmoid portion, and 12 (17.1 %) were investigated beyond the sigmoid colon. All of the cases showed at least 1 of the 4 characteristics in the rectosigmoid portion. The percentage of crypt apoptosis in the biopsy specimen from orange peel appearance, spotty redness, small mucosal sloughing, and diffuse mucosal defect were 87.5 %, 83.3 %, 87.2 %, and 88.9 %, respectively. Conclusion  Orange peel appearance, spotty redness, small mucosal sloughing, and diffuse mucosal defect are the characteristic colonoscopic findings useful for diagnosis of gastrointestinal GVHD. These findings are frequently observed in the rectosigmoid portion. The histological detection rates for crypt cell apoptosis from these findings are high. Identifying the four characteristic findings on rectosigmoidoscopy and taking biopsies from these areas could be essential for the diagnostic strategy for gastrointestinal GVHD. PMID:29507868

Crash Frequency Modeling Using Real-Time Environmental and Traffic Data and Unbalanced Panel Data Models

PubMed Central

Chen, Feng; Chen, Suren; Ma, Xiaoxiang

2016-01-01

Traffic and environmental conditions (e.g., weather conditions), which frequently change with time, have a significant impact on crash occurrence. Traditional crash frequency models with large temporal scales and aggregated variables are not sufficient to capture the time-varying nature of driving environmental factors, causing significant loss of critical information on crash frequency modeling. This paper aims at developing crash frequency models with refined temporal scales for complex driving environments, with such an effort providing more detailed and accurate crash risk information which can allow for more effective and proactive traffic management and law enforcement intervention. Zero-inflated, negative binomial (ZINB) models with site-specific random effects are developed with unbalanced panel data to analyze hourly crash frequency on highway segments. The real-time driving environment information, including traffic, weather and road surface condition data, sourced primarily from the Road Weather Information System, is incorporated into the models along with site-specific road characteristics. The estimation results of unbalanced panel data ZINB models suggest there are a number of factors influencing crash frequency, including time-varying factors (e.g., visibility and hourly traffic volume) and site-varying factors (e.g., speed limit). The study confirms the unique significance of the real-time weather, road surface condition and traffic data to crash frequency modeling. PMID:27322306

Combining density functional theory (DFT) and collision cross-section (CCS) calculations to analyze the gas-phase behaviour of small molecules and their protonation site isomers.

PubMed

Boschmans, Jasper; Jacobs, Sam; Williams, Jonathan P; Palmer, Martin; Richardson, Keith; Giles, Kevin; Lapthorn, Cris; Herrebout, Wouter A; Lemière, Filip; Sobott, Frank

2016-06-20

Electrospray ion mobility-mass spectrometry (IM-MS) data show that for some small molecules, two (or even more) ions with identical sum formula and mass, but distinct drift times are observed. In spite of showing their own unique and characteristic fragmentation spectra in MS/MS, no configurational or constitutional isomers are found to be present in solution. Instead the observation and separation of such ions appears to be inherent to their gas-phase behaviour during ion mobility experiments. The origin of multiple drift times is thought to be the result of protonation site isomers ('protomers'). Although some important properties of protomers have been highlighted by other studies, correlating the experimental collision cross-sections (CCSs) with calculated values has proven to be a major difficulty. As a model, this study uses the pharmaceutical compound melphalan and a number of related molecules with alternative (gas-phase) protonation sites. Our study combines density functional theory (DFT) calculations with modified MobCal methods (e.g. nitrogen-based Trajectory Method algorithm) for the calculation of theoretical CCS values. Calculated structures can be linked to experimentally observed signals, and a strong correlation is found between the difference of the calculated dipole moments of the protomer pairs and their experimental CCS separation.

Assessment of Gaseous Oxidized Mercury Measurement Accuracy at an Atmospheric Mercury Network (AMNet) Site

NASA Astrophysics Data System (ADS)

Luke, W. T.

2016-12-01

Recent laboratory and field research has documented and explored the biases and inaccuracies of the measurement of gaseous oxidized mercury (GOM) compounds using KCl-coated denuders. We report on the development of a simple, automated GOM calibration source and its deployment at NOAA/Air Resources Laboratory's Atmospheric Mercury Network (AMNet) site at the Mauna Loa Observatory (MLO) on the island of Hawaii. NOAA/ARL has developed a permeation-tube based calibration source with an extremely simple flow path that minimizes surface adsorptive effects and losses. The source was used to inject HgBr2 into one of two side-by-side Tekran® mercury speciation systems at MLO to characterize GOM measurement accuracy under a variety of atmospheric conditions. Due to its unique topography and meteorology, MLO experiences katabatic (upslope/downslope) mesoscale flow superimposed on the synoptic trade wind circulation of the tropics. Water vapor, ozone, and other trace atmospheric constituents often display pronounced diurnal variations at the site, which frequently encounters air characteristic of the middle free troposphere at night, and of the tropical marine boundary layer during the day. Results presented here will assist in the better understanding of the biases underlying GOM measurements in global mercury monitoring networks and may allow the development of correction factors for ambient data.

Site characteristics and prey abundance at foraging sites used by Lesser Scaup (Aythya affinis) wintering in Florida

USGS Publications Warehouse

Herring, Garth; Collazo, Jaime

2009-01-01

We examined site characteristics and prey abundances where wintering Aythya affinis (Lesser Scaup; hereafter scaup) foraged within three regions of the Indian River Lagoon system in central Florida. We observed that scaup concentrated in the Indian and Banana rivers; however, density of prey items did not differ between foraging sites and random sites. We also found that site characteristics were similar between foraging and random sites. Differences in site characteristics between random points across all three regions did not explain the distribution of Foraging scaup (no scaup foraged in the Mosquito Lagoon); however, prey densities were approximately 3 times lower in the Mosquito Lagoon region. Our study suggests that current habitat conditions within the northern Indian River Lagoon system meet the overwintering requirements of scaup; however, prey densities in the Mosquito Lagoon may have been too low to be profitable for foraging scaup during the period of our study.

Microbial biogeography of wine grapes is conditioned by cultivar, vintage, and climate

PubMed Central

Bokulich, Nicholas A.; Thorngate, John H.; Richardson, Paul M.; Mills, David A.

2014-01-01

Wine grapes present a unique biogeography model, wherein microbial biodiversity patterns across viticultural zones not only answer questions of dispersal and community maintenance, they are also an inherent component of the quality, consumer acceptance, and economic appreciation of a culturally important food product. On their journey from the vineyard to the wine bottle, grapes are transformed to wine through microbial activity, with indisputable consequences for wine quality parameters. Wine grapes harbor a wide range of microbes originating from the surrounding environment, many of which are recognized for their role in grapevine health and wine quality. However, determinants of regional wine characteristics have not been identified, but are frequently assumed to stem from viticultural or geological factors alone. This study used a high-throughput, short-amplicon sequencing approach to demonstrate that regional, site-specific, and grape-variety factors shape the fungal and bacterial consortia inhabiting wine-grape surfaces. Furthermore, these microbial assemblages are correlated to specific climatic features, suggesting a link between vineyard environmental conditions and microbial inhabitation patterns. Taken together, these factors shape the unique microbial inputs to regional wine fermentations, posing the existence of nonrandom “microbial terroir” as a determining factor in regional variation among wine grapes. PMID:24277822

The microbiomes and metagenomes of forest biochars

NASA Astrophysics Data System (ADS)

Noyce, Genevieve L.; Winsborough, Carolyn; Fulthorpe, Roberta; Basiliko, Nathan

2016-05-01

Biochar particles have been hypothesized to provide unique microhabitats for a portion of the soil microbial community, but few studies have systematically compared biochar communities to bulk soil communities. Here, we used a combination of sequencing techniques to assess the taxonomic and functional characteristics of microbial communities in four-year-old biochar particles and in adjacent soils across three forest environments. Though effects varied between sites, the microbial community living in and around the biochar particles had significantly lower prokaryotic diversity and higher eukaryotic diversity than the surrounding soil. In particular, the biochar bacterial community had proportionally lower abundance of Acidobacteria, Planctomycetes, and β-Proteobacteria taxa, compared to the soil, while the eukaryotic biochar community had an 11% higher contribution of protists belonging to the Aveolata superphylum. Additionally, we were unable to detect a consistent biochar effect on the genetic functional potential of these microbial communities for the subset of the genetic data for which we were able to assign functions through MG-RAST. Overall, these results show that while biochar particles did select for a unique subset of the biota found in adjacent soils, effects on the microbial genetic functional potential appeared to be specific to contrasting forest soil environments.

Implant bone integration importance in forensic identification.

PubMed

De Angelis, Danilo; Cattaneo, Cristina

2015-03-01

Odontological identification consists of the comparison of antemortem dental information regarding a missing person with postmortem data from an unidentified corpse or human remains. Usually, the comparison concerns morphologic features that the operator chooses among all the visible characteristics because of inter-individual uniqueness; for this reason, implants can be of enormous assistance. A case concerning the recovery of a burnt oral implant, connected to a bone fragment, among 2780 charred bone fragments, suspected to have belonged to a victim of homicide, is presented to demonstrate that dental implants and their site of bone integration represent a very precious element for personal forensic identification. Because of their morphological invariability in time and because of their morphologic uniqueness, they were used as evidence to associate unidentified human charred remains to a missing person where DNA analysis failed to do so. The case illustrates the fundamental contribution, not yet described in literature, given by the clinical aspects of tooth replacement with dental implants to a forensic discipline. Clinical practitioners should therefore be aware of the great importance of their work and of dental records in a forensic identification scenario. © 2014 American Academy of Forensic Sciences.

Microbial biogeography of wine grapes is conditioned by cultivar, vintage, and climate.

PubMed

Bokulich, Nicholas A; Thorngate, John H; Richardson, Paul M; Mills, David A

2014-01-07

Wine grapes present a unique biogeography model, wherein microbial biodiversity patterns across viticultural zones not only answer questions of dispersal and community maintenance, they are also an inherent component of the quality, consumer acceptance, and economic appreciation of a culturally important food product. On their journey from the vineyard to the wine bottle, grapes are transformed to wine through microbial activity, with indisputable consequences for wine quality parameters. Wine grapes harbor a wide range of microbes originating from the surrounding environment, many of which are recognized for their role in grapevine health and wine quality. However, determinants of regional wine characteristics have not been identified, but are frequently assumed to stem from viticultural or geological factors alone. This study used a high-throughput, short-amplicon sequencing approach to demonstrate that regional, site-specific, and grape-variety factors shape the fungal and bacterial consortia inhabiting wine-grape surfaces. Furthermore, these microbial assemblages are correlated to specific climatic features, suggesting a link between vineyard environmental conditions and microbial inhabitation patterns. Taken together, these factors shape the unique microbial inputs to regional wine fermentations, posing the existence of nonrandom "microbial terroir" as a determining factor in regional variation among wine grapes.

The microbiomes and metagenomes of forest biochars

PubMed Central

Noyce, Genevieve L.; Winsborough, Carolyn; Fulthorpe, Roberta; Basiliko, Nathan

2016-01-01

Biochar particles have been hypothesized to provide unique microhabitats for a portion of the soil microbial community, but few studies have systematically compared biochar communities to bulk soil communities. Here, we used a combination of sequencing techniques to assess the taxonomic and functional characteristics of microbial communities in four-year-old biochar particles and in adjacent soils across three forest environments. Though effects varied between sites, the microbial community living in and around the biochar particles had significantly lower prokaryotic diversity and higher eukaryotic diversity than the surrounding soil. In particular, the biochar bacterial community had proportionally lower abundance of Acidobacteria, Planctomycetes, and β-Proteobacteria taxa, compared to the soil, while the eukaryotic biochar community had an 11% higher contribution of protists belonging to the Aveolata superphylum. Additionally, we were unable to detect a consistent biochar effect on the genetic functional potential of these microbial communities for the subset of the genetic data for which we were able to assign functions through MG-RAST. Overall, these results show that while biochar particles did select for a unique subset of the biota found in adjacent soils, effects on the microbial genetic functional potential appeared to be specific to contrasting forest soil environments. PMID:27212657

The natural armors of fish: A comparison of the lamination pattern and structure of scales

DOE Office of Scientific and Technical Information (OSTI.GOV)

Murcia, Sandra; Lavoie, Ellen; Linley, Tim

Fish scales exhibit a unique balance of flexibility, strength and toughness, which is essential to provide protection without encumbering locomotion. Although the mechanical behavior and structure of this natural armor are of recent interest, a comparison of these qualities from scales of different fish species has not been reported. In this investigation the armor of fish with different locomotion, size and protection needs were analyzed. Scales from the Arapaima gigas, the tarpon (Megalops atlanticus) and the carp (Cyprinus carpio) were compared in terms of the stacking sequence of individual plies and their microstructure. The scales were also compared with respectmore » to anatomical position to distinguish site-specific functional differences. Results show that the lamination sequence of plies for the carp and tarpon exhibit a Bouligand structure with relative rotation of 75° between consecutive plies. The arapaima scales exhibit a cross-ply structure, with 90° rotation between adjacent plies. In addition, results indicate that the volume fraction of reinforcement, the number of plies and the variations in thickness with anatomical position are unique amongst the three fish. These characteristics should be considered in evaluations focused on the mechanical behavior.« less

Development of a Dynamically Scaled Generic Transport Model Testbed for Flight Research Experiments

NASA Technical Reports Server (NTRS)

Jordan, Thomas; Langford, William; Belcastro, Christine; Foster, John; Shah, Gautam; Howland, Gregory; Kidd, Reggie

2004-01-01

This paper details the design and development of the Airborne Subscale Transport Aircraft Research (AirSTAR) test-bed at NASA Langley Research Center (LaRC). The aircraft is a 5.5% dynamically scaled, remotely piloted, twin-turbine, swept wing, Generic Transport Model (GTM) which will be used to provide an experimental flight test capability for research experiments pertaining to dynamics modeling and control beyond the normal flight envelope. The unique design challenges arising from the dimensional, weight, dynamic (inertial), and actuator scaling requirements necessitated by the research community are described along with the specific telemetry and control issues associated with a remotely piloted subscale research aircraft. Development of the necessary operational infrastructure, including operational and safety procedures, test site identification, and research pilots is also discussed. The GTM is a unique vehicle that provides significant research capacity due to its scaling, data gathering, and control characteristics. By combining data from this testbed with full-scale flight and accident data, wind tunnel data, and simulation results, NASA will advance and validate control upset prevention and recovery technologies for transport aircraft, thereby reducing vehicle loss-of-control accidents resulting from adverse and upset conditions.

In Situ Preparation of Biomimetic Thin Films and Their Surface-Shielding Effect for Organisms in High Vacuum

PubMed Central

Muranaka, Yoshinori; Shimomura, Masatsugu; Hariyama, Takahiko

2013-01-01

Self-standing biocompatible films have yet to be prepared by physical or chemical vapor deposition assisted by plasma polymerization because gaseous monomers have thus far been used to create only polymer membranes. Using a nongaseous monomer, we previously found a simple fabrication method for a free-standing thin film prepared from solution by plasma polymerization, and a nano-suit made by polyoxyethylene (20) sorbitan monolaurate can render multicellular organisms highly tolerant to high vacuum. Here we report thin films prepared by plasma polymerization from various monomer solutions. The films had a flat surface at the irradiated site and were similar to films produced by vapor deposition of gaseous monomers. However, they also exhibited unique characteristics, such as a pinhole-free surface, transparency, solvent stability, flexibility, and a unique out-of-plane molecular density gradient from the irradiated to the unirradiated surface of the film. Additionally, covering mosquito larvae with the films protected the shape of the organism and kept them alive under the high vacuum conditions in a field emission-scanning electron microscope. Our method will be useful for numerous applications, particularly in the biological sciences. PMID:24236023

In situ sensing of subsurface contamination--part I: near-infrared spectral characterization of alkanes, aromatics, and chlorinated hydrocarbons.

PubMed

Klavarioti, Maria; Kostarelos, Konstantinos; Pourjabbar, Anahita; Ghandehari, Masoud

2014-05-01

There is an imperative need for a chemical sensor capable of remote, in situ, long-term monitoring of chemical species at sites containing toxic chemical spills, specifically at chemical waste dumps, landfills, and locations with underground storage tanks. In the current research, a series of experiments were conducted measuring the near-infrared optical absorption of alkanes, aromatics, and chlorinated hydrocarbons. A spectral library was then developed to characterize the optical spectra of liquid hydrocarbons. Near-infrared analysis was chosen due to compatibility with optical fibers. The goal was to differentiate between classes of hydrocarbons and to also discriminate between compounds within a class of similar molecular structures. It was observed that unique absorption spectra can be obtained for each hydrocarbon, and this uniqueness can be used to discriminate between hydrocarbons from different families. Statistical analyses, namely, principal component analysis (PCA) and correlation coefficient (Spearman and Pearson methods), were attempted to match absorption spectra from an unknown hydrocarbon with the database with limited success. An algorithm was subsequently written to identify the characteristic peaks of each hydrocarbon that could be used to match data from an unknown chemical species with the database.

Stable isotope, site-specific mass tagging for protein identification

DOEpatents

Chen, Xian

2006-10-24

Proteolytic peptide mass mapping as measured by mass spectrometry provides an important method for the identification of proteins, which are usually identified by matching the measured and calculated m/z values of the proteolytic peptides. A unique identification is, however, heavily dependent upon the mass accuracy and sequence coverage of the fragment ions generated by peptide ionization. The present invention describes a method for increasing the specificity, accuracy and efficiency of the assignments of particular proteolytic peptides and consequent protein identification, by the incorporation of selected amino acid residue(s) enriched with stable isotope(s) into the protein sequence without the need for ultrahigh instrumental accuracy. Selected amino acid(s) are labeled with .sup.13C/.sup.15N/.sup.2H and incorporated into proteins in a sequence-specific manner during cell culturing. Each of these labeled amino acids carries a defined mass change encoded in its monoisotopic distribution pattern. Through their characteristic patterns, the peptides with mass tag(s) can then be readily distinguished from other peptides in mass spectra. The present method of identifying unique proteins can also be extended to protein complexes and will significantly increase data search specificity, efficiency and accuracy for protein identifications.

Performance Art at the Campusphere: Pedagogical Experiments On-Site

ERIC Educational Resources Information Center

Ben-Shaul, Daphna

2018-01-01

Following a unique practice and research laboratory entitled "Performance: Site/Self" that took place in 2013-2015, this article discusses the implementation of performance art at an academic site--the Tel Aviv University campus. This pedagogical and artistic initiative, characterised by the transgressive pedagogy of performance art…

Effects of Source RDP Models and Near-source Propagation: Implication for Seismic Yield Estimation

NASA Astrophysics Data System (ADS)

Saikia, C. K.; Helmberger, D. V.; Stead, R. J.; Woods, B. B.

- It has proven difficult to uniquely untangle the source and propagation effects on the observed seismic data from underground nuclear explosions, even when large quantities of near-source, broadband data are available for analysis. This leads to uncertainties in our ability to quantify the nuclear seismic source function and, consequently the accuracy of seismic yield estimates for underground explosions. Extensive deterministic modeling analyses of the seismic data recorded from underground explosions at a variety of test sites have been conducted over the years and the results of these studies suggest that variations in the seismic source characteristics between test sites may be contributing to the observed differences in the magnitude/yield relations applicable at those sites. This contributes to our uncertainty in the determination of seismic yield estimates for explosions at previously uncalibrated test sites. In this paper we review issues involving the relationship of Nevada Test Site (NTS) source scaling laws to those at other sites. The Joint Verification Experiment (JVE) indicates that a magnitude (mb) bias (δmb) exists between the Semipalatinsk test site (STS) in the former Soviet Union (FSU) and the Nevada test site (NTS) in the United States. Generally this δmb is attributed to differential attenuation in the upper-mantle beneath the two test sites. This assumption results in rather large estimates of yield for large mb tunnel shots at Novaya Zemlya. A re-examination of the US testing experiments suggests that this δmb bias can partly be explained by anomalous NTS (Pahute) source characteristics. This interpretation is based on the modeling of US events at a number of test sites. Using a modified Haskell source description, we investigated the influence of the source Reduced Displacement Potential (RDP) parameters ψ ∞ , K and B by fitting short- and long-period data simultaneously, including the near-field body and surface waves. In general, estimates of B and K are based on the initial P-wave pulse, which various numerical analyses show to be least affected by variations in near-source path effects. The corner-frequency parameter K is 20% lower at NTS (Pahute) than at other sites, implying larger effective source radii. The overshoot parameter B appears to be low at NTS (although variable) relative to other sites and is probably due to variations in source conditions. For a low B, the near-field data require a higher value of ψ ∞ to match the long-period MS and short-period mb observations. This flexibility in modeling proves useful in comparing released FSU yields against predictions based on mb and MS.

Hydromorphological parameters of natural channel behavior in conditions of the Hercynian System and the flysch belt of the Western Carpathians on the territory of the Czech Republic

NASA Astrophysics Data System (ADS)

Kujanová, Kateřina; Matoušková, Milada; Kliment, Zdeněk

2016-04-01

A fundamental prerequisite for assessing the current ecological status of streams is the establishment of reference conditions for each stream type that serve as a benchmark. The hydromorphological reference conditions reflect the natural channel behavior, which is extremely variable. Significant parameters of natural channel behavior were determined using a combination of four selected statistical methods: Principal Component Analysis, Agglomerative Hierarchical Clustering, correlation, and regression. Macroscale analyses of data about altitude, stream order, channel slope, valley floor slope, sinuosity, and characteristics of the hydrological regime were conducted for 3197 reaches of major rivers in the Czech Republic with total length of 15,636 km. On the basis of selected significant parameters and their threshold values, channels were classified into groups of river characteristics based on shared behaviors. The channel behavior within these groups was validated using hydromorphological characteristics of natural channels determined during field research at reference sites. Classification of channels into groups confirmed the fundamental differences between channel behavior under conditions of the Hercynian System and the flysch belt of the Western Carpathians in the Czech Republic and determined a specific group in the flattened high areas of mountains in the Bohemian Massif. Validating confirmed the distinctions between groups of river characteristics and the uniqueness of each one; it also emphasized the benefits of using qualitative data and riparian zone characteristics for describing channel behavior. Channel slope, entrenchment ratio, bed structure, and d50 were determined as quantitative characteristics of natural channel behavior.

The lost micro-deserts of the Patuxent River using landscape history, insect and plant specimens, and field work to detect and define a unique community

USGS Publications Warehouse

Droege, S.; Davis, C.A.; Steiner, W.E.; =Mawdsley, J.

2009-01-01

Historical and recent records of both plants and insects are synthesized for uplands along the eastern edge of Maryland?s Patuxent River from the edge of the Piedmont south to Jug Bay. This strip is characterized by deep sandy soils found in the Evesboro and Galestown sandy loams soil series. Within this narrow strip there exists a unique flora and fauna adapted to open dry sandy soils and occurring in small remnant patches associated with old sand mining operations and scattered protected areas. We illustrate the uniqueness of these sites using four groups, vascular plants, tenebrionid beetles (Coleoptera: Tenebrionidae), tiger beetles (Coleoptera: Cicindelidae), and bees (Hymenoptera: Apoidea: Anthophila). Within each of these groups, rare species were detected whose populations were locally restricted to this soil type and whose nearest known populations were often hundreds of kilometers away. In addition to documenting the direct conservation importance of these small sandy openings along the Patuxent, we contrast the lack of any indication from vertebrate inventories that this region is unique. The combination of plant and insect inventories appears to be a better means of clarifying a site?s importance than does any survey of a single taxonomic group.

Infiltration Processes and Flow Velocities Across the Landscape: When and Where is Macropore Flow Relevant?

NASA Astrophysics Data System (ADS)

Demand, D.; Blume, T.; Weiler, M.

2017-12-01

Preferential flow in macropores significantly affects the distributions of water and solutes in soil and many studies showed its relevance worldwide. Although some models include this process as a second pore domain, little is known about the spatial patterns and temporal dynamics. For example, while flow in the matrix is usually modeled and parameterized based on soil texture, an influence of texture on non-capillary flow for a given land-use class is poorly understood. To investigate the temporal and spatial dynamics on preferential flow we used a four-year soil moisture dataset from the mesoscale Attert catchment (288 km²) in Luxembourg. This dataset contains time series from 126 soil profiles in different textures and two land-use classes (forest, grassland). The soil moisture probes were installed in 10, 30 and 50 cm depth and measured in a 5-minute temporal resolution. Events were defined by a soil moisture increase higher than the instrument noise after a precipitation sum of more than 1 mm. Precipitation was measured next to the profiles so that each location could be associated to its unique precipitation characteristics. For every event and profile the soil moisture reaction was classified in sequential (ordered by depth) and non-sequential response. A non-sequential soil moisture reaction was used as an indicator of preferential flow. For sequential flow, the velocity was determined by the first reaction between two vertically adjacent sensors. The sensor reaction and wetting front velocity was analyzed in the context of precipitation characteristics and initial soil water content. Grassland sites showed a lower proportion of non-sequential flow than forest sites. For forest, non-sequential response is dependent on texture, rainfall intensity and initial water content. This is less distinct for the grassland sites. Furthermore, sequential reactions show higher flow velocities at sites, which also have high percentage of non-sequential response. In contrast, grassland sites show a more homogenous wetting front independent of soil texture. Compared against common modelling approaches of soil water flow, measured velocities show clear evidence of preferential flow, especially for forest soils. The analysis also shows that vegetation can alter the soil properties above the textural properties alone.

Hydrologic Source Term Processes and Models for the Clearwater and Wineskin Tests, Rainier Mesa, Nevada National Security Site

DOE Office of Scientific and Technical Information (OSTI.GOV)

Carle, Steven F.

2011-05-04

This report describes the development, processes, and results of a hydrologic source term (HST) model for the CLEARWATER (U12q) and WINESKIN (U12r) tests located on Rainier Mesa, Nevada National Security Site, Nevada (Figure 1.1). Of the 61 underground tests (involving 62 unique detonations) conducted on Rainier Mesa (Area 12) between 1957 and 1992 (USDOE, 2015), the CLEARWATER and WINESKIN tests present many unique features that warrant a separate HST modeling effort from other Rainier Mesa tests.

Formation and Evolution of Lakshmi Planum (V-7), Venus: Assessment of Models using Observations from Geological Mapping

NASA Technical Reports Server (NTRS)

Ivanov, M. A.; Head, James W.

2008-01-01

Lakshmi Planum is a high-standing plateau (3.5-4.5 km above MPR) surrounded by the highest mountain ranges on Venus. Lakshmi represents a unique type of elevated region different from dome-shaped and rifted rises and tessera-bearing crustal plateaus. The unique characteristics of Lakshmi suggest that it formed by an unusual combination of processes and played an important role in Venus geologic history. Lakshmi was studied with Venera-15/16 and Magellan data, resulting in two classes of models, divergent and convergent, to explain its unusual topographic and morphologic characteristics. Divergent models explain Lakshmi as a site of mantle upwelling due to rising and subsequent collapse of a mantle diapir; such models explain emplacement of a lava plateau inside Lakshmi and, in some circumstances, formation of the mountain ranges. The convergent models consider Lakshmi as a locus of mantle downwelling, convergence, underthrusting, and possible subduction. Key features in these models are the mountain ranges, high topography of Lakshmi interior, and the large volcanic centers in the plateau center. These divergent and convergent models entail principally different mechanisms of formation and suggest different geodynamic regimes on Venus. Almost all models make either explicit or implicit predictions about the type and sequence of major events during formation and evolution of Lakshmi and thus detailed geological mapping can be used to test them. Here we present the results of such geological mapping (the V-7 quadrangle, 50-75degN, 300-360degE; scale 1:5M) that allows testing the proposed models for Lakshmi.

16 CFR 1031.18 - Method of review and comment.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Commission staff is involved shall have a unique Web link on the Commission Web site with relevant... forwarded to appropriate staff for consideration and/or response. (c) On the voluntary standards Web site...

16 CFR 1031.18 - Method of review and comment.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Commission staff is involved shall have a unique Web link on the Commission Web site with relevant... forwarded to appropriate staff for consideration and/or response. (c) On the voluntary standards Web site...

16 CFR 1031.18 - Method of review and comment.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Commission staff is involved shall have a unique Web link on the Commission Web site with relevant... forwarded to appropriate staff for consideration and/or response. (c) On the voluntary standards Web site...

Unique Prokaryotic Consortia in Geochemically Distinct Sediments from Red Sea Atlantis II and Discovery Deep Brine Pools

PubMed Central

Siam, Rania; Mustafa, Ghada A.; Sharaf, Hazem; Moustafa, Ahmed; Ramadan, Adham R.; Antunes, Andre; Bajic, Vladimir B.; Stingl, Uli; Marsis, Nardine G. R.; Coolen, Marco J. L.; Sogin, Mitchell; Ferreira, Ari J. S.; Dorry, Hamza El

2012-01-01

The seafloor is a unique environment, which allows insights into how geochemical processes affect the diversity of biological life. Among its diverse ecosystems are deep-sea brine pools - water bodies characterized by a unique combination of extreme conditions. The ‘polyextremophiles’ that constitute the microbial assemblage of these deep hot brines have not been comprehensively studied. We report a comparative taxonomic analysis of the prokaryotic communities of the sediments directly below the Red Sea brine pools, namely, Atlantis II, Discovery, Chain Deep, and an adjacent brine-influenced site. Analyses of sediment samples and high-throughput pyrosequencing of PCR-amplified environmental 16S ribosomal RNA genes (16S rDNA) revealed that one sulfur (S)-rich Atlantis II and one nitrogen (N)-rich Discovery Deep section contained distinct microbial populations that differed from those found in the other sediment samples examined. Proteobacteria, Actinobacteria, Cyanobacteria, Deferribacteres, and Euryarchaeota were the most abundant bacterial and archaeal phyla in both the S- and N-rich sections. Relative abundance-based hierarchical clustering of the 16S rDNA pyrotags assigned to major taxonomic groups allowed us to categorize the archaeal and bacterial communities into three major and distinct groups; group I was unique to the S-rich Atlantis II section (ATII-1), group II was characteristic for the N-rich Discovery sample (DD-1), and group III reflected the composition of the remaining sediments. Many of the groups detected in the S-rich Atlantis II section are likely to play a dominant role in the cycling of methane and sulfur due to their phylogenetic affiliations with bacteria and archaea involved in anaerobic methane oxidation and sulfate reduction. PMID:22916172

Analysis of Traffic Signals on a Software-Defined Network for Detection and Classification of a Man-in-the-Middle Attack

DTIC Science & Technology

2017-09-01

unique characteristics of reported anomalies in the collected traffic signals to build a classification framework. Other cyber events, such as a...Furthermore, we identify unique characteristics of reported anomalies in the collected traffic signals to build a classification framework. Other cyber...2]. The applications build flow rules using network topology information provided by the control plane [1]. Since the control plane is able to

Flow-Signature Analysis of Water Consumption in Nonresidential Building Water Networks Using High-Resolution and Medium-Resolution Smart Meter Data: Two Case Studies

NASA Astrophysics Data System (ADS)

Clifford, Eoghan; Mulligan, Sean; Comer, Joanne; Hannon, Louise

2018-01-01

Real-time monitoring of water consumption activities can be an effective mechanism to achieve efficient water network management. This approach, largely enabled by the advent of smart metering technologies, is gradually being practiced in domestic and industrial contexts. In particular, identifying water consumption habits from flow-signatures, i.e., the specific end-usage patterns, is being investigated as a means for conservation in both the residential and nonresidential context. However, the quality of meter data is bivariate (dependent on number of meters and data temporal resolution) and as a result, planning a smart metering scheme is relatively difficult with no generic design approach available. In this study, a comprehensive medium-resolution to high-resolution smart metering program was implemented at two nonresidential trial sites to evaluate the effect of spatial and temporal data aggregation. It was found that medium-resolution water meter data were capable of exposing regular, continuous, peak use, and diurnal patterns which reflect group wide end-usage characteristics. The high-resolution meter data permitted flow-signature at a personal end-use level. Through this unique opportunity to observe water usage characteristics via flow-signature patterns, newly defined hydraulic-based design coefficients determined from Poisson rectangular pulse were developed to intuitively aid in the process of pattern discovery with implications for automated activity recognition applications. A smart meter classification and siting index was introduced which categorizes meter resolution in terms of their suitable application.

Observational study of atmospheric surface layer and coastal weather in northern Qatar

NASA Astrophysics Data System (ADS)

Samanta, Dhrubajyoti; Sadr, Reza

2016-04-01

Atmospheric surface layer is the interaction medium between atmosphere and Earth's surface. Better understanding of its turbulence nature is essential in characterizing the local weather, climate variability and modeling of turbulent exchange processes. The importance of Middle East region, with its unique geographical, economical and weather condition is well recognized. However, high quality micrometeorological observational studies are rare in this region. Here we show experimental results from micrometeorological observations from an experimental site in the coastal region of Qatar during August-December 2015. Measurements of winds are obtained from three sonic anemometers installed on a 9 m tower placed at Al Ghariyah beach in northern Qatar (26.08 °N, 51.36 °E). Different surface layer characteristics is analyzed and compared with earlier studies in equivalent weather conditions. Monthly statistics of wind speed, wind direction, temperature, humidity and heat index are made from concurrent observations from sonic anemometer and weather station to explore variations with surface layer characteristics. The results also highlights potential impact of sea breeze circulation on local weather and atmospheric turbulence. The observed daily maximum temperature and heat index during morning period may be related to sea breeze circulations. Along with the operational micrometeorological observation system, a camera system and ultrasonic wave measurement system are installed recently in the site to study coastline development and nearshore wave dynamics. Overall, the complete observational set up is going to provide new insights about nearshore wind dynamics and wind-wave interaction in Qatar.

Are BTK and PLCG2 mutations necessary and sufficient for ibrutinib resistance in chronic lymphocytic leukemia?

PubMed

Lampson, Benjamin L; Brown, Jennifer R

2018-03-01

Ibrutinib is the first BTK inhibitor to show efficacy in chronic lymphocytic leukemia (CLL) and is also the first BTK inhibitor to which patients have developed resistance. Mutations in BTK and PLCG2 are found in ≈80% of CLL patients with acquired resistance to ibrutinib, but it remains unclear if these mutations are merely associated with disease relapse or directly cause it. Areas covered: Unique properties of both CLL and ibrutinib that complicate attempts to definitively conclude whether BTK/PLCG2 mutations are passengers or drivers of ibrutinib-resistant disease are reviewed. Characteristics of mutations that drive drug resistance are summarized and whether BTK/PLCG2 mutations possess these is discussed. These characteristics include (1) identification in multiple patients with acquired resistance, (2) in vitro validation of drug-resistant properties, (3) mutual exclusivity with one another, (4) increasing frequency over time on drug, and (5) high frequency at the time and site of clinical relapse. Expert commentary: While BTK/PLCG2 mutations have characteristics suggesting that they can drive ibrutinib resistance, this conclusion remains formally unproven until specific inhibition of such mutations is shown to cause regression of ibrutinib-resistant CLL. Data suggest that alternative mechanisms of resistance do exist in some patients.

Deepest and hottest hydrothermal activity in the Okinawa Trough: the Yokosuka site at Yaeyama Knoll

PubMed Central

Miyazaki, Junichi; Kawagucci, Shinsuke; Makabe, Akiko; Takahashi, Ayu; Kitada, Kazuya; Torimoto, Junji; Matsui, Yohei; Tasumi, Eiji; Shibuya, Takazo; Nakamura, Kentaro; Horai, Shunsuke; Sato, Shun; Ishibashi, Jun-ichiro; Kanzaki, Hayato; Nakagawa, Satoshi; Hirai, Miho; Takaki, Yoshihiro; Okino, Kyoko; Watanabe, Hiromi Kayama; Kumagai, Hidenori

2017-01-01

Since the initial discovery of hydrothermal vents in 1977, these ‘extreme’ chemosynthetic systems have been a focus of interdisciplinary research. The Okinawa Trough (OT), located in the semi-enclosed East China Sea between the Eurasian continent and the Ryukyu arc, hosts more than 20 known vent sites but all within a relatively narrow depth range (600–1880 m). Depth is a significant factor in determining fluid temperature and chemistry, as well as biological composition. However, due to the narrow depth range of known sites, the actual influence of depth here has been poorly resolved. Here, the Yokosuka site (2190 m), the first OT vent exceeding 2000 m depth is reported. A highly active hydrothermal vent site centred around four active vent chimneys reaching 364°C in temperature, it is the hottest in the OT. Notable Cl depletion (130 mM) and both high H2 and CH4 concentrations (approx. 10 mM) probably result from subcritical phase separation and thermal decomposition of sedimentary organic matter. Microbiota and fauna were generally similar to other sites in the OT, although with some different characteristics. In terms of microbiota, the H2-rich vent fluids in Neuschwanstein chimney resulted in the dominance of hydrogenotrophic chemolithoautotrophs such as Thioreductor and Desulfobacterium. For fauna, the dominance of the deep-sea mussel Bathymodiolus aduloides is surprising given other nearby vent sites are usually dominated by B. platifrons and/or B. japonicus, and a sponge field in the periphery dominated by Poecilosclerida is unusual for OT vents. Our insights from the Yokosuka site implies that although the distribution of animal species may be linked to depth, the constraint is perhaps not water pressure and resulting chemical properties of the vent fluid but instead physical properties of the surrounding seawater. The potential significance of these preliminary results and prospect for future research on this unique site are discussed. PMID:29308272

[Effect of forest management on the herpetofauna of a temperate forest of western Oaxaca, Mexico].

PubMed

Aldape-López, César Tonatiuh; Santos-Moreno, Antonio

2016-09-01

The development of silvicultural techniques has as main objective to maximize the production of timber, whereas at the same time minimize the impact generated during and after forest intervention in the local diversity. However, these activities change local climate, and this, in turn, alter the composition of natural communities. The effect of these changes may be greater in those taxonomic groups with high sensitivity to habitat disturbance, such as amphibians and reptiles, which are the unique terrestrial ectothermic vertebrates. The present study aims to know the differences in diversity of amphibians and reptiles in a temperate forest under two silvicultural treatments, one of low and the other of high intensity, as well as from one, five and ten years of regeneration since the last logging event, Sierra Sur of Oaxaca, Mexico. Records of 21 species of herpetofauna (six amphibians and 15 reptiles) were obtained. The total species richness was similar in both treatments; however, the composition varied between sites with different recovery times. Higher abundance of amphibian was presented on sites with the low-intensity treatment, while reptiles were more abundant at sites with intensive treatment. Compared to a mature forest without management, sites with intensive treatment have more rare species, although the values of true diversity of amphibians were similar between treatments with different intensities, while for reptiles sites under treatment showed less diversity that unmanaged site: 33 % for intensive treatment and 28 % at sites with low intensity with respect to one control site. Complementary Analysis showed a difference of 86 % between the compositions of species in sites with intensive treatment. The treatment intensity was associated with an increase in the number of species, but the way they respond to changes in habitat depends largely on the population characteristics of each species and its ability to adapt to new conditions.

Deepest and hottest hydrothermal activity in the Okinawa Trough: the Yokosuka site at Yaeyama Knoll

NASA Astrophysics Data System (ADS)

Miyazaki, Junichi; Kawagucci, Shinsuke; Makabe, Akiko; Takahashi, Ayu; Kitada, Kazuya; Torimoto, Junji; Matsui, Yohei; Tasumi, Eiji; Shibuya, Takazo; Nakamura, Kentaro; Horai, Shunsuke; Sato, Shun; Ishibashi, Jun-ichiro; Kanzaki, Hayato; Nakagawa, Satoshi; Hirai, Miho; Takaki, Yoshihiro; Okino, Kyoko; Watanabe, Hiromi Kayama; Kumagai, Hidenori; Chen, Chong

2017-12-01

Since the initial discovery of hydrothermal vents in 1977, these `extreme' chemosynthetic systems have been a focus of interdisciplinary research. The Okinawa Trough (OT), located in the semi-enclosed East China Sea between the Eurasian continent and the Ryukyu arc, hosts more than 20 known vent sites but all within a relatively narrow depth range (600-1880 m). Depth is a significant factor in determining fluid temperature and chemistry, as well as biological composition. However, due to the narrow depth range of known sites, the actual influence of depth here has been poorly resolved. Here, the Yokosuka site (2190 m), the first OT vent exceeding 2000 m depth is reported. A highly active hydrothermal vent site centred around four active vent chimneys reaching 364°C in temperature, it is the hottest in the OT. Notable Cl depletion (130 mM) and both high H2 and CH4 concentrations (approx. 10 mM) probably result from subcritical phase separation and thermal decomposition of sedimentary organic matter. Microbiota and fauna were generally similar to other sites in the OT, although with some different characteristics. In terms of microbiota, the H2-rich vent fluids in Neuschwanstein chimney resulted in the dominance of hydrogenotrophic chemolithoautotrophs such as Thioreductor and Desulfobacterium. For fauna, the dominance of the deep-sea mussel Bathymodiolus aduloides is surprising given other nearby vent sites are usually dominated by B. platifrons and/or B. japonicus, and a sponge field in the periphery dominated by Poecilosclerida is unusual for OT vents. Our insights from the Yokosuka site implies that although the distribution of animal species may be linked to depth, the constraint is perhaps not water pressure and resulting chemical properties of the vent fluid but instead physical properties of the surrounding seawater. The potential significance of these preliminary results and prospect for future research on this unique site are discussed.

Identification of a unique Ca2+-binding site in rat acid-sensing ion channel 3.

PubMed

Zuo, Zhicheng; Smith, Rachel N; Chen, Zhenglan; Agharkar, Amruta S; Snell, Heather D; Huang, Renqi; Liu, Jin; Gonzales, Eric B

2018-05-25

Acid-sensing ion channels (ASICs) evolved to sense changes in extracellular acidity with the divalent cation calcium (Ca 2+ ) as an allosteric modulator and channel blocker. The channel-blocking activity is most apparent in ASIC3, as removing Ca 2+ results in channel opening, with the site's location remaining unresolved. Here we show that a ring of rat ASIC3 (rASIC3) glutamates (Glu435), located above the channel gate, modulates proton sensitivity and contributes to the formation of the elusive Ca 2+ block site. Mutation of this residue to glycine, the equivalent residue in chicken ASIC1, diminished the rASIC3 Ca 2+ block effect. Atomistic molecular dynamic simulations corroborate the involvement of this acidic residue in forming a high-affinity Ca 2+ site atop the channel pore. Furthermore, the reported observations provide clarity for past controversies regarding ASIC channel gating. Our findings enhance understanding of ASIC gating mechanisms and provide structural and energetic insights into this unique calcium-binding site.

The challenge of spin–orbit-tuned ground states in iridates: a key issues review

NASA Astrophysics Data System (ADS)

Cao, Gang; Schlottmann, Pedro

2018-04-01

Effects of spin–orbit interactions in condensed matter are an important and rapidly evolving topic. Strong competition between spin–orbit, on-site Coulomb and crystalline electric field interactions in iridates drives exotic quantum states that are unique to this group of materials. In particular, the ‘J eff  =  ½’ Mott state served as an early signal that the combined effect of strong spin–orbit and Coulomb interactions in iridates has unique, intriguing consequences. In this Key Issues Review, we survey some current experimental studies of iridates. In essence, these materials tend to defy conventional wisdom: absence of conventional correlations between magnetic and insulating states, avoidance of metallization at high pressures, ‘S-shaped’ I–V characteristic, emergence of an odd-parity hidden order, etc. It is particularly intriguing that there exist conspicuous discrepancies between current experimental results and theoretical proposals that address superconducting, topological and quantum spin liquid phases. This class of materials, in which the lattice degrees of freedom play a critical role seldom seen in other materials, evidently presents some profound intellectual challenges that call for more investigations both experimentally and theoretically. Physical properties unique to these materials may help unlock a world of possibilities for functional materials and devices. We emphasize that, given the rapidly developing nature of this field, this Key Issues Review is by no means an exhaustive report of the current state of experimental studies of iridates.

Barriers, facilitators, and benefits of implementation of dialectical behavior therapy in routine care: results from a national program evaluation survey in the Veterans Health Administration.

PubMed

Landes, Sara J; Rodriguez, Allison L; Smith, Brandy N; Matthieu, Monica M; Trent, Lindsay R; Kemp, Janet; Thompson, Caitlin

2017-12-01

National implementation of evidence-based psychotherapies (EBPs) in the Veterans Health Administration (VHA) provides important lessons on the barriers and facilitators to implementation in a large healthcare system. Little is known about barriers and facilitators to the implementation of a complex EBP for emotional and behavioral dysregulation-dialectical behavioral therapy (DBT). The purpose of this study was to understand VHA clinicians' experiences with barriers, facilitators, and benefits from implementing DBT into routine care. This national program evaluation survey measured site characteristics of VHA sites (N = 59) that had implemented DBT. DBT was most often implemented in general mental health outpatient clinics. While 42% of sites offered all four modes of DBT, skills group was the most frequently implemented mode. Fifty-nine percent of sites offered phone coaching in any form, yet only 11% of those offered it all the time. Providers were often provided little to no time to support implementation of DBT. Barriers that were difficult to overcome were related to phone coaching outside of business hours. Facilitators to implementation included staff interest and expertise. Perceived benefits included increased hope and functioning for clients, greater self-efficacy and compassion for providers, and ability to treat unique symptoms for clinics. There was considerable variability in the capacity to address implementation barriers among sites implementing DBT in VHA routine care. Mental health policy makers should note the barriers and facilitators reported here, with specific attention to phone coaching barriers.

Long-term urban carbon dioxide observations reveal spatial and temporal dynamics related to urban characteristics and growth

DOE PAGES

Mitchell, Logan E.; Lin, John C.; Bowling, David R.; ...

2018-03-05

Cities are concentrated areas of CO 2 emissions and have become the foci of policies for mitigation actions. However, atmospheric measurement networks suitable for evaluating urban emissions over time are scarce. Here we present a unique long-term (decadal) record of CO 2 mole fractions from five sites across Utah’s metropolitan Salt Lake Valley. We examine “excess” CO 2 above background conditions resulting from local emissions and meteorological conditions. We ascribe CO 2 trends to changes in emissions, since we did not find longterm trends in atmospheric mixing proxies. Three contrasting CO 2 trends emerged across urban types: negative trends atmore » a residentialindustrial site, positive trends at a site surrounded by rapid suburban growth, and relatively constant CO 2 over time at multiple sites in the established, residential, and commercial urban core. Analysis of populationwithin the atmospheric footprints of the different sites reveals approximately equal increases in population influencing the observed CO 2, implying a nonlinear relationshipwith CO 2 emissions: Population growth in rural areas that experienced suburban development was associated with increasing emissions while population growth in the developed urban core was associated with stable emissions. Four state-of-the-art global-scale emission inventories also have a nonlinear relationship with population density across the city; however, in contrast to our observations, they all have nearly constant emissions over time. Our results indicate that decadal scale changes in urban CO 2 emissions are detectable through monitoring networks and constitute a valuable approach to evaluate emission inventories and studies of urban carbon cycles.« less

Long-term urban carbon dioxide observations reveal spatial and temporal dynamics related to urban characteristics and growth

NASA Astrophysics Data System (ADS)

Mitchell, Logan E.; Lin, John C.; Bowling, David R.; Pataki, Diane E.; Strong, Courtenay; Schauer, Andrew J.; Bares, Ryan; Bush, Susan E.; Stephens, Britton B.; Mendoza, Daniel; Mallia, Derek; Holland, Lacey; Gurney, Kevin R.; Ehleringer, James R.

2018-03-01

Cities are concentrated areas of CO2 emissions and have become the foci of policies for mitigation actions. However, atmospheric measurement networks suitable for evaluating urban emissions over time are scarce. Here we present a unique long-term (decadal) record of CO2 mole fractions from five sites across Utah’s metropolitan Salt Lake Valley. We examine “excess” CO2 above background conditions resulting from local emissions and meteorological conditions. We ascribe CO2 trends to changes in emissions, since we did not find long-term trends in atmospheric mixing proxies. Three contrasting CO2 trends emerged across urban types: negative trends at a residential-industrial site, positive trends at a site surrounded by rapid suburban growth, and relatively constant CO2 over time at multiple sites in the established, residential, and commercial urban core. Analysis of population within the atmospheric footprints of the different sites reveals approximately equal increases in population influencing the observed CO2, implying a nonlinear relationship with CO2 emissions: Population growth in rural areas that experienced suburban development was associated with increasing emissions while population growth in the developed urban core was associated with stable emissions. Four state-of-the-art global-scale emission inventories also have a nonlinear relationship with population density across the city; however, in contrast to our observations, they all have nearly constant emissions over time. Our results indicate that decadal scale changes in urban CO2 emissions are detectable through monitoring networks and constitute a valuable approach to evaluate emission inventories and studies of urban carbon cycles.

Occurrence of Polyphenols, Organic Acids, and Sugars among Diverse Elderberry Genotypes Grown in Three Missouri (USA) Locations

PubMed Central

Thomas, A.L.; Byers, P.L.; Gu, S.; Avery, J.D.; Kaps, M.; Datta, A.; Fernando, L.; Grossi, P.; Rottinghaus, G.E.

2016-01-01

Elderberry (Sambucus spp.) is an emerging horticultural crop used in a variety of foods, wines, and dietary supplements. A better understanding of the elderberry juice complex including its putative health-promoting compounds in relation to genetic and environmental parameters is needed. A multi-location planting of nine elderberry genotypes was established in 2008 at three geographically-diverse sites in Missouri, USA. Fruits were harvested from replicated plots 2009-2011, frozen, and later prepared for laboratory analysis. Polyphenols, organic acids, and sugars were quantified by HPLC and the results evaluated for response to genotype, site, and year. The American genotypes ‘Ocoee’ and ‘Ozark’ were consistently higher in chlorogenic acids compared to other genotypes, whereas ‘Ocoee’ was significantly higher in rutin than ‘Ozark’. The European ‘Marge’ was significantly higher in isoquercitrin and other flavonoids compared to most North American genotypes. Significant differences in polyphenols were also detected among sites and production years. Malic, citric, and tartaric acids varied significantly among genotypes, sites, and years, whereas succinic, shikimic, and fumaric acids generally did not. Levels of lactic, acetic, and propionic acids were negligible in most samples. The American genotype ‘Ocoee’ was higher in citric and tartaric acids, while lower in malic acid. The sugars glucose and fructose also responded significantly to genotype, site, and year. ‘Ocoee’, ‘Ozark’, and ‘Marge’ perform very well in Missouri horticulturally and appear to have additional potential as cultivars based on their unique juice characteristics. PMID:27156707

Long-term urban carbon dioxide observations reveal spatial and temporal dynamics related to urban characteristics and growth

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mitchell, Logan E.; Lin, John C.; Bowling, David R.

Cities are concentrated areas of CO 2 emissions and have become the foci of policies for mitigation actions. However, atmospheric measurement networks suitable for evaluating urban emissions over time are scarce. Here we present a unique long-term (decadal) record of CO 2 mole fractions from five sites across Utah’s metropolitan Salt Lake Valley. We examine “excess” CO 2 above background conditions resulting from local emissions and meteorological conditions. We ascribe CO 2 trends to changes in emissions, since we did not find longterm trends in atmospheric mixing proxies. Three contrasting CO 2 trends emerged across urban types: negative trends atmore » a residentialindustrial site, positive trends at a site surrounded by rapid suburban growth, and relatively constant CO 2 over time at multiple sites in the established, residential, and commercial urban core. Analysis of populationwithin the atmospheric footprints of the different sites reveals approximately equal increases in population influencing the observed CO 2, implying a nonlinear relationshipwith CO 2 emissions: Population growth in rural areas that experienced suburban development was associated with increasing emissions while population growth in the developed urban core was associated with stable emissions. Four state-of-the-art global-scale emission inventories also have a nonlinear relationship with population density across the city; however, in contrast to our observations, they all have nearly constant emissions over time. Our results indicate that decadal scale changes in urban CO 2 emissions are detectable through monitoring networks and constitute a valuable approach to evaluate emission inventories and studies of urban carbon cycles.« less

Role of block copolymer-micelle nanocomposites in dye-bovine serum albumin binding: a combined experimental and molecular docking study.

PubMed

Manna, Anamika; Chakravorti, Sankar

2013-02-02

The role of a nanocomposite (NC), composed of intercalation of the diblock copolymer polyethylene-b-polyethylene glycol (PE-b-PEG) with the anionic surfactant sodium dodecyl sulphate (SDS), on the binding characteristics of bovine serum albumin (BSA) with a dye (1,8-naphthalimide, NAPMD) compared to the interaction between the same players in aqueous solution has been examined comprehensively in this paper. Static quenching due to complex formation in both NC medium and in buffer solution has been inferred on the basis of considerable changes in the absorption spectra of BSA on addition of NAPMD, of which the interaction is found to be stronger in NC medium. Temperature dependent fluorescence data also confirm an effective static quenching and stronger binding of NAPMD with BSA in NC medium. Peptide chain unfolding and denaturing of BSA in NC medium have been confirmed from steady state and time-resolved emission and circular dichroism data. This exposes both the tyrosine and tryptophan moieties as a unique case. Increased energy transfer between NAPMD and the tryptophan residue in the unfolded form of BSA helps in the appearance of tyrosine fluorescence in NC medium by quenching the tryptophan band. Ionization of the hydroxyl group in the aromatic ring of the tyrosine residue by the PEG group present in the NC medium produces a downshift of the tyrosine fluorescence band. The use of site selective markers confirms that NAPMD is near tryptophan in Sudlow's site I in NC medium and in buffer solution it is away from tryptophan in Sudlow's site II. The theoretical docking studies also vindicate the results of binding of NAPMD with BSA in site I or site II in NC and buffer media, as observed from different emission experiments including the site selective markers study.

Identifying potential selective fluorescent probes for cancer-associated protein carbonic anhydrase IX using a computational approach.

PubMed

Kamstra, Rhiannon L; Floriano, Wely B

2014-11-01

Carbonic anhydrase IX (CAIX) is a biomarker for tumor hypoxia. Fluorescent inhibitors of CAIX have been used to study hypoxic tumor cell lines. However, these inhibitor-based fluorescent probes may have a therapeutic effect that is not appropriate for monitoring treatment efficacy. In the search for novel fluorescent probes that are not based on known inhibitors, a database of 20,860 fluorescent compounds was virtually screened against CAIX using hierarchical virtual ligand screening (HierVLS). The screening database contained 14,862 compounds tagged with the ATTO680 fluorophore plus an additional 5998 intrinsically fluorescent compounds. Overall ranking of compounds to identify hit molecular probe candidates utilized a principal component analysis (PCA) approach. Four potential binding sites, including the catalytic site, were identified within the structure of the protein and targeted for virtual screening. Available sequence information for 23 carbonic anhydrase isoforms was used to prioritize the four sites based on the estimated "uniqueness" of each site in CAIX relative to the other isoforms. A database of 32 known inhibitors and 478 decoy compounds was used to validate the methodology. A receiver-operating characteristic (ROC) analysis using the first principal component (PC1) as predictive score for the validation database yielded an area under the curve (AUC) of 0.92. AUC is interpreted as the probability that a binder will have a better score than a non-binder. The use of first component analysis of binding energies for multiple sites is a novel approach for hit selection. The very high prediction power for this approach increases confidence in the outcome from the fluorescent library screening. Ten of the top scoring candidates for isoform-selective putative binding sites are suggested for future testing as fluorescent molecular probe candidates. Copyright © 2014 Elsevier Inc. All rights reserved.

KatB, a cyanobacterial Mn-catalase with unique active site configuration: Implications for enzyme function.

PubMed

Bihani, Subhash C; Chakravarty, Dhiman; Ballal, Anand

2016-04-01

Manganese catalases (Mn-catalases), a class of H2O2 detoxifying proteins, are structurally and mechanistically distinct from the commonly occurring catalases, which contain heme. Active site of Mn-catalases can serve as template for the synthesis of catalase mimetics for therapeutic intervention in oxidative stress related disorders. However, unlike the heme catalases, structural aspects of Mn-catalases remain inadequately explored. The genome of the ancient cyanobacterium Anabaena PCC7120, shows the presence of two Mn-catalases, KatA and KatB. Here, we report the biochemical and structural characterization of KatB. The KatB protein (with a C-terminal his-tag) was over-expressed in Escherichia coli and purified by affinity chromatography. On the addition of Mn(2+) to the E. coli growth medium, a substantial increase in production of the soluble KatB protein was observed. The purified KatB protein was an efficient catalase, which was relatively insensitive to inhibition by azide. Crystal structure of KatB showed a hexameric assembly with four-helix bundle fold, characteristic of the Ferritin-like superfamily. With canonical Glu4His2 coordination geometry and two terminal water ligands, the KatB active site was distinctly different from that of other Mn-catalases. Interestingly, the KatB active site closely resembled the active sites of ruberythrin/bacterioferritin, bi-iron members of the Ferritin-like superfamily. The KatB crystal structure provided fundamental insights into the evolutionary relationship within the Ferritin-like superfamily and further showed that Mn-catalases can be sub-divided into two groups, each with a distinct active site configuration. Copyright © 2016 Elsevier Inc. All rights reserved.

Divergent Drinking Patterns of Restaurant Workers: The Influence of Social Networks and Job Position.

PubMed

Duke, Michael R; Ames, Genevieve M; Moore, Roland S; Cunradi, Carol B

2013-01-01

Restaurant workers have higher rates of problem drinking than most occupational groups. However, little is known about the environmental risks and work characteristics that may lead to these behaviors. An exploration of restaurant workers' drinking networks may provide important insights into their alcohol consumption patterns, thus guiding workplace prevention efforts. Drawing from social capital theory, this paper examines the unique characteristics of drinking networks within and between various job categories. Our research suggests that these multiple, complex networks have unique risk characteristics, and that self-selection is based on factors such as job position and college attendance, among other factors.

Divergent Drinking Patterns of Restaurant Workers: The Influence of Social Networks and Job Position

PubMed Central

Ames, Genevieve M.; Moore, Roland S.; Cunradi, Carol B.

2013-01-01

Restaurant workers have higher rates of problem drinking than most occupational groups. However, little is known about the environmental risks and work characteristics that may lead to these behaviors. An exploration of restaurant workers’ drinking networks may provide important insights into their alcohol consumption patterns, thus guiding workplace prevention efforts. Drawing from social capital theory, this paper examines the unique characteristics of drinking networks within and between various job categories. Our research suggests that these multiple, complex networks have unique risk characteristics, and that self-selection is based on factors such as job position and college attendance, among other factors. PMID:23687470

Raising Awareness of Individual Creative Potential in Bioscientists Using a Web-Site Based Approach

ERIC Educational Resources Information Center

Adams, David J.; Hugh-Jones, Siobhan; Sutherland, Ed

2010-01-01

We report the preliminary results of work with a unique, web-site-based approach designed to help individual bioscientists identify and develop their individual creative capacity. The site includes a number of features that encourage individuals to interact with creativity techniques, communicate with colleagues remotely using an electronic notice…

Blue Ribbon Web Sites Contest Winners.

ERIC Educational Resources Information Center

Southworth, Samuel A.

2001-01-01

Presents a collection of prize-winning Web sites created by K-8 teachers nationwide. Some of the unique features of the Web sites include an online student-written newspaper; a sing-along section; a chronicle of the past 3 years of classes to see how the classes have evolved; and student art and writing projects. (SM)

Moving Forward: Two Paradigms and Takeaways

ERIC Educational Resources Information Center

Rocco, Tonette S.

2011-01-01

Disability is not something one experiences in isolation of other characteristics. Sociocultural characteristics, such as race, class, gender, sexual orientation, age and disability, interact with each other in various combinations forming unique adults. When interacting with other characteristics, disability can dominate the other…

Comprehensive Characterization a Tidal Energy Site (Invited)

NASA Astrophysics Data System (ADS)

Polagye, B. L.; Thomson, J. M.; Bassett, C. S.; Epler, J.; Northwest National Marine Renewable Energy Center

2010-12-01

Northern Admiralty Inlet, Puget Sound, Washington is the proposed location of a pilot tidal energy project. Site-specific characterization of the physical and biological environment is required for device engineering and environmental analysis. However, the deep water and strong currents which make the site attractive for tidal energy development also pose unique challenges to collecting comprehensive information. This talk focuses on efforts to optimally site hydrokinetic turbines and estimate their acoustic impact, based on 18 months of field data collected to date. Additional characterization efforts being undertaken by the University of Washington branch of the Northwest National Marine Renewable Energy Center and its partners include marine mammal presence and behavior, water quality, seabed geology, and biofouling potential. Because kinetic power density varies with the cube of horizontal current velocity, an accurate map of spatial current variations is required to optimally site hydrokinetic turbines. Acoustic Doppler profilers deployed on the seabed show operationally meaningful variations in flow characteristics (e.g., power density, directionality, vertical shear) and tidal harmonic constituents over length scales of less than 100m. This is, in part, attributed to the proximity of this site to a headland. Because of these variations, interpolation between stationary measurement locations introduces potentially high uncertainty. The use of shipboard acoustic Doppler profilers is shown to be an effective tool for mapping peak currents and, combined with information from seabed profilers, may be able to resolve power density variations in the project area. Because noise levels from operating turbines are expected to exceed regulatory thresholds for incidental harassment of marine mammals known to be present in the project area, an estimate of the acoustic footprint is required to permit the pilot project. This requires site-specific descriptions of pre-existing ambient noise levels and the transmission loss (or practical spreading) at frequencies of interest. Recording hydrophones deployed on the seabed are used to quantify ambient noise, but are contaminated by self-noise during periods of strong currents. An empirical estimate of transmission loss is obtained from a source of opportunity - a passenger ferry which operates for more than twelve hours each day. By comparing recorded sound pressure levels against the location of the passenger ferry and other vessels (logged by an AIS receiver), the empirical transmission loss and source level for the ferry are obtained. Measurements of current velocity and underwater noise can apply routine oceanographic instruments and techniques. More unique measurements will be more challenging, such as high resolution sampling of current structure upstream and downstream of an operating device tens of meters off the seabed. Innovative approaches are required for cost effective characterization of tidal energy sites and monitoring of operating projects.

Personomics: The Missing Link in the Evolution from Precision Medicine to Personalized Medicine.

PubMed

Ziegelstein, Roy C

2017-10-16

Clinical practice guidelines have been developed for many common conditions based on data from randomized controlled trials. When medicine is informed solely by clinical practice guidelines, however, the patient is not treated as an individual, but rather a member of a group. Precision medicine, as defined herein, characterizes unique biological characteristics of the individual or of specimens obtained from an individual to tailor diagnostics and therapeutics to a specific patient. These unique biological characteristics are defined by the tools of precision medicine: genomics, proteomics, metabolomics, epigenomics, pharmacogenomics, and other "-omics." Personalized medicine, as defined herein, uses additional information about the individual derived from knowing the patient as a person. These unique personal characteristics are defined by tools known as personomics which takes into account an individual's personality, preferences, values, goals, health beliefs, social support network, financial resources, and unique life circumstances that affect how and when a given health condition will manifest in that person and how that condition will respond to treatment. In this paradigm, precision medicine may be considered a necessary step in the evolution of medical care to personalized medicine, with personomics as the missing link.

Chapter A. The Loma Prieta, California, Earthquake of October 17, 1989 - Strong Ground Motion

USGS Publications Warehouse

Borcherdt, Roger D.

1994-01-01

Strong ground motion generated by the Loma Prieta, Calif., earthquake (MS~7.1) of October 17, 1989, resulted in at least 63 deaths, more than 3,757 injuries, and damage estimated to exceed $5.9 billion. Strong ground motion severely damaged critical lifelines (freeway overpasses, bridges, and pipelines), caused severe damage to poorly constructed buildings, and induced a significant number of ground failures associated with liquefaction and landsliding. It also caused a significant proportion of the damage and loss of life at distances as far as 100 km from the epicenter. Consequently, understanding the characteristics of the strong ground motion associated with the earthquake is fundamental to understanding the earthquake's devastating impact on society. The papers assembled in this chapter address this problem. Damage to vulnerable structures from the earthquake varied substantially with the distance from the causative fault and the type of underlying geologic deposits. Most of the damage and loss of life occurred in areas underlain by 'soft soil'. Quantifying these effects is important for understanding the tragic concentrations of damage in such areas as Santa Cruz and the Marina and Embarcadero Districts of San Francisco, and the failures of the San Francisco-Oakland Bay Bridge and the Interstate Highway 880 overpass. Most importantly, understanding these effects is a necessary prerequisite for improving mitigation measures for larger earthquakes likely to occur much closer to densely urbanized areas in the San Francisco Bay region. The earthquake generated an especially important data set for understanding variations in the severity of strong ground motion. Instrumental strong-motion recordings were obtained at 131 sites located from about 6 to 175 km from the rupture zone. This set of recordings, the largest yet collected for an event of this size, was obtained from sites on various geologic deposits, including a unique set on 'soft soil' deposits (artificial fill and bay mud). These exceptional ground-motion data are used by the authors of the papers in this chapter to infer radiation characteristics of the earthquake source, identify dominant propagation characteristics of the Earth?s crust, quantify amplification characteristics of near-surface geologic deposits, develop general amplification factors for site-dependent building-code provisions, and revise earthquake-hazard assessments for the San Francisco Bay region. Interpretations of additional data recorded in well-instrumented buildings, dams, and freeway overpasses are provided in other chapters of this report.

Integrating peatlands into the coupled Canadian Land Surface Scheme (CLASS) v3.6 and the Canadian Terrestrial Ecosystem Model (CTEM) v2.0

NASA Astrophysics Data System (ADS)

Wu, Yuanqiao; Verseghy, Diana L.; Melton, Joe R.

2016-08-01

Peatlands, which contain large carbon stocks that must be accounted for in the global carbon budget, are poorly represented in many earth system models. We integrated peatlands into the coupled Canadian Land Surface Scheme (CLASS) and the Canadian Terrestrial Ecosystem Model (CTEM), which together simulate the fluxes of water, energy, and CO2 at the land surface-atmosphere boundary in the family of Canadian Earth system models (CanESMs). New components and algorithms were added to represent the unique features of peatlands, such as their characteristic ground floor vegetation (mosses), the slow decomposition of carbon in the water-logged soils and the interaction between the water, energy, and carbon cycles. This paper presents the modifications introduced into the CLASS-CTEM modelling framework together with site-level evaluations of the model performance for simulated water, energy and carbon fluxes at eight different peatland sites. The simulated daily gross primary production (GPP) and ecosystem respiration are well correlated with observations, with values of the Pearson correlation coefficient higher than 0.8 and 0.75 respectively. The simulated mean annual net ecosystem production at the eight test sites is 87 g C m-2 yr-1, which is 22 g C m-2 yr-1 higher than the observed annual mean. The general peatland model compares well with other site-level and regional-level models for peatlands, and is able to represent bogs and fens under a range of climatic and geographical conditions.

Integrating peatlands into the coupled Canadian Land Surface Scheme (CLASS) v3.6 and the Canadian Terrestrial Ecosystem Model (CTEM) v2.0

NASA Astrophysics Data System (ADS)

Wu, Y.; Verseghy, D. L.; Melton, J. R.

2015-11-01

Peatlands, which contain large carbon stocks that must be accounted for in the global carbon budget, are poorly represented in many earth system models. We integrated peatlands into the coupled Canadian Land Surface Scheme (CLASS) and the Canadian Terrestrial Ecosystem Model (CTEM), which together simulate the fluxes of water, energy and CO2 at the land surface-atmosphere boundary in the family of Canadian Earth System Models (CanESMs). New components and algorithms were added to represent the unique features of peatlands, such as their characteristic ground floor vegetation (mosses), the slow decomposition of carbon in the water-logged soils and the interaction between the water, energy and carbon cycles. This paper presents the modifications introduced into the CLASS-CTEM modelling framework together with site-level evaluations of the model performance for simulated water, energy and carbon fluxes at eight different peatland sites. The simulated daily gross primary production and ecosystem respiration are well correlated with observations, with values of the Pearson correlation coefficient higher than 0.8 and 0.75 respectively. The simulated mean annual net ecosystem production at the eight test sites is 87 g C m-2 yr-1, which is 22 g C m-2 yr-1 higher than the observed annual mean. The general peatland model compares well with other site-level and regional-level models for peatlands, and is able to represent bogs and fens under a range of climatic and geographical conditions.

The unexploded ordnance (UXO) contamination problem and the state of the art of UXO remediation technologies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Thomsen, K.O.; Richardson, C.B.; Valder, K.M.

1996-12-31

Millions of acres of US government property are contaminated with unexploded ordnance (UXO) as a result of weapons system testing and troop training activities conducted over the past century at Department of Defense (DoD) sites. Recent DoD downsizing has resulted in the closing of many military bases, many of which are contaminated with UXO. One unexpected result of DoD`s downsizing is the attention focused on the unique problems associated with UXO remediation at these closed military bases. The U.S. Army Environmental Center (U SAEC) is the lead DoD agency for UXO clearance technology demonstrations, evaluation, and technology transfer. USAEC directedmore » the Naval Explosive Ordnance Disposal Technology Division (NAVEODTECHDIV) to serve as the technical lead for the advanced technology demonstration (ATD) program. In 1994, USAEC and NAVEODTECHDIV created controlled test facilities at the U.S. Army Jefferson Proving Ground in Madison, Indiana, to demonstrate and evaluate commercial UXO clearance systems and technologies. Phase I controlled site demonstrations were conducted during the summer of 1994. These demonstrations were followed by the Phase II controlled site demonstrations at JPG. This paper presents the results of the Phase II controlled site demonstrations. The overall performance of the demonstrators is presented along with the operational characteristics and limitations of the various systems and technologies evaluated. Individual demonstrator performance statistics are evaluated by sensor type and sensor transport method.« less

Early biocompatibility of crosslinked and non-crosslinked biologic meshes in a porcine model of ventral hernia repair.

PubMed

Melman, L; Jenkins, E D; Hamilton, N A; Bender, L C; Brodt, M D; Deeken, C R; Greco, S C; Frisella, M M; Matthews, B D

2011-04-01

Biologic meshes have unique physical properties as a result of manufacturing techniques such as decellularization, crosslinking, and sterilization. The purpose of this study is to directly compare the biocompatibility profiles of five different biologic meshes, AlloDerm(®) (non-crosslinked human dermal matrix), PeriGuard(®) (crosslinked bovine pericardium), Permacol(®) (crosslinked porcine dermal matrix), Strattice(®) (non-crosslinked porcine dermal matrix), and Veritas(®) (non-crosslinked bovine pericardium), using a porcine model of ventral hernia repair. Full-thickness fascial defects were created in 20 Yucatan minipigs and repaired with the retromuscular placement of biologic mesh 3 weeks later. Animals were euthanized at 1 month and the repair sites were subjected to tensile testing and histologic analysis. Samples of unimplanted (de novo) meshes and native porcine abdominal wall were also analyzed for their mechanical properties. There were no significant differences in the biomechanical characteristics between any of the mesh-repaired sites at 1 month postimplantation or between the native porcine abdominal wall without implanted mesh and the mesh-repaired sites (P > 0.05 for all comparisons). Histologically, non-crosslinked materials exhibited greater cellular infiltration, extracellular matrix (ECM) deposition, and neovascularization compared to crosslinked meshes. While crosslinking differentiates biologic meshes with regard to cellular infiltration, ECM deposition, scaffold degradation, and neovascularization, the integrity and strength of the repair site at 1 month is not significantly impacted by crosslinking or by the de novo strength/stiffness of the mesh.

Perceptual Characteristics of Female Voices.

ERIC Educational Resources Information Center

Batstone, Susan; Tuomi, Seppo K.

1981-01-01

Male and females listeners rated 21 young female voices on seven scales representing unique vocal features. Voices were described as "passive", or traditionally female, and "active," characterized as "lively,""colorful," and "sexy." Females found active characteristics more salient; males preferred the passive characteristics. Implications for…

Accumulation of slightly deleterious mutations in the mitochondrial genome: a hallmark of animal domestication.

PubMed

Hughes, Austin L

2013-02-15

The hypothesis that domestication leads to a relaxation of purifying selection on mitochondrial (mt) genomes was tested by comparative analysis of mt genes from dog, pig, chicken, and silkworm. The three vertebrate species showed mt genome phylogenies in which domestic and wild isolates were intermingled, whereas the domestic silkworm (Bombyx mori) formed a distinct cluster nested within its closest wild relative (Bombyx mandarina). In spite of these differences in phylogenetic pattern, significantly greater proportions of nonsynonymous SNPs than of synonymous SNPs were unique to the domestic populations of all four species. Likewise, in all four species, significantly greater proportions of RNA-encoding SNPs than of synonymous SNPs were unique to the domestic populations. Thus, domestic populations were characterized by an excess of unique polymorphisms in two categories generally subject to purifying selection: nonsynonymous sites and RNA-encoding sites. Many of these unique polymorphisms thus seem likely to be slightly deleterious; the latter hypothesis was supported by the generally lower gene diversities of polymorphisms unique to domestic populations in comparison to those of polymorphisms shared by domestic and wild populations. Copyright © 2012 Elsevier B.V. All rights reserved.

Computerized stratified random site-selection approaches for design of a ground-water-quality sampling network

USGS Publications Warehouse

Scott, J.C.

1990-01-01

Computer software was written to randomly select sites for a ground-water-quality sampling network. The software uses digital cartographic techniques and subroutines from a proprietary geographic information system. The report presents the approaches, computer software, and sample applications. It is often desirable to collect ground-water-quality samples from various areas in a study region that have different values of a spatial characteristic, such as land-use or hydrogeologic setting. A stratified network can be used for testing hypotheses about relations between spatial characteristics and water quality, or for calculating statistical descriptions of water-quality data that account for variations that correspond to the spatial characteristic. In the software described, a study region is subdivided into areal subsets that have a common spatial characteristic to stratify the population into several categories from which sampling sites are selected. Different numbers of sites may be selected from each category of areal subsets. A population of potential sampling sites may be defined by either specifying a fixed population of existing sites, or by preparing an equally spaced population of potential sites. In either case, each site is identified with a single category, depending on the value of the spatial characteristic of the areal subset in which the site is located. Sites are selected from one category at a time. One of two approaches may be used to select sites. Sites may be selected randomly, or the areal subsets in the category can be grouped into cells and sites selected randomly from each cell.

Development of tough, moisture resistant laminating resins

NASA Technical Reports Server (NTRS)

Brand, R. A.; Harrison, E. S.

1982-01-01

Tough, moisture resistant laminating resins for employment with graphite fibers were developed. The new laminating resins exhibited cost, handleability and processing characteristics equivalent to 394K (250 F) curing epoxies. The laminating resins were based on bisphenol A dicyanate and monofunctional cyanates with hydrophobic substituents. These resins sorb only small quantities of moisture at equilibrium (0.5% or less) with minimal glass transition temperature depression and represent an improvement over epoxies which sorb around 2% moisture at equilibrium. Toughening was accomplished by the precipitation of small diameter particles of butadiene nitrile rubber throughout the resin matrix. The rubber domains act as microcrack termini and energy dissipation sites, allowing increased stress accommodation prior to catastrophic failure. A unique blend of amine terminated butadiene nitrile elastomer (MW 2,000) and a high nitrile content butadiene nitrile rubber yielded the desired resin morphology.

DNA fragment sizing and sorting by laser-induced fluorescence

DOEpatents

Hammond, Mark L.; Jett, James H.; Keller, Richard A.; Marrone, Babetta L.; Martin, John C.

1996-01-01

A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.

Inhibitor-binding mode of homobelactosin C to proteasomes: New insights into class I MHC ligand generation

PubMed Central

Groll, Michael; Larionov, Oleg V.; Huber, Robert; de Meijere, Armin

2006-01-01

Most class I MHC ligands are generated from the vast majority of cellular proteins by proteolysis within the ubiquitin–proteasome pathway and are presented on the cell surface by MHC class I molecules. Here, we present the crystallographic analysis of yeast 20S proteasome in complex with the inhibitor homobelactosin C. The structure reveals a unique inhibitor-binding mode and provides information about the composition of proteasomal primed substrate-binding sites. IFN-γ inducible substitution of proteasomal constitutive subunits by immunosubunits modulates characteristics of generated peptides, thus producing fragments with higher preference for binding to MHC class I molecules. The structural data for the proteasome:homobelactosin C complex provide an explanation for involvement of immunosubunits in antigen generation and open perspectives for rational design of ligands, inhibiting exclusively constitutive proteasomes or immunoproteasomes. PMID:16537370

PNA binding to the non-template DNA strand interferes with transcription, suggesting a blockage mechanism mediated by R-loop formation.

PubMed

Belotserkovskii, Boris P; Hanawalt, Philip C

2015-11-01

Peptide Nucleic Acids (PNAs) are artificial DNA mimics with superior nucleic acid binding capabilities. T7 RNA polymerase (T7 RNAP) transcription upon encountering PNA bound to the non-template DNA strand was studied in vitro. A characteristic pattern of blockage signals was observed, extending downstream from the PNA binding site, similar to that produced by G-rich homopurine-homopyrimidine (hPu-hPy) sequences and likely caused by R-loop formation. Since blocked transcription complexes in association with stable R-loops may interfere with replication and in some cases trigger apoptosis, targeted R-loop formation might be employed to inactivate selected cells, such as those in tumors, based upon their unique complement of expressed genes. © 2014 The Authors. Molecular Carcinogenesis published by Wiley Periodicals, Inc.

Enhanced photocurrent in engineered bacteriorhodopsin monolayer.

PubMed

Patil, Amol V; Premaruban, Thenhuan; Berthoumieu, Olivia; Watts, Anthony; Davis, Jason J

2012-01-12

The integration of the transmembrane protein bacteriorhodopsin (BR) with man-made electrode surfaces has attracted a great deal of interest for some two decades or more and holds significant promise from the perspective of derived photoresponse or energy capture interfaces. Here we demonstrate that a novel and strategically engineered cysteine site (M163C) can be used to intimately and effectively couple delipidated BR to supporting metallic electrode surfaces. By virtue of the combined effects of the greater surface molecular density afforded by delipidation, and the vicinity of the electrostatic changes associated with proton pumping to the transducing metallic continuum, the resulting films generate a considerably greater photocurrent density on wavelength-selective illumination than previously achievable with monolayers of BR. Given the uniquely photoresponsive, wavelength-selective, and photostable characteristics of this protein, the work has implications for utilization in solar energy capture and photodetector devices.

The characteristics and experiences of anticipatory mourning in caregivers of teenagers and young adults.

PubMed

Griffith, Rachel; Davies, Kerry; Lavender, Verna

2015-11-01

This article reports a systematic review of literature undertaken to identify characteristics and experiences of anticipatory mourning in caregivers of teenagers and young adults with life-limiting or life-threatening conditions. A comprehensive literature search was conducted using the key words 'anticipatory', 'mourning', 'grief', and synonyms. This review focused on six studies that met inclusion criteria and reported characteristics of anticipatory mourning in caregivers of teenagers and young adults. Characteristics and experiences were sorted into four main themes: symptoms; a sense of loss; caregiver behaviour; and the unique experience of caring for, or losing, a teenager or young adult. The review suggests that there are characteristics and experiences of anticipatory mourning that are unique to caregivers of this age group. The review also suggests that consideration of anticipatory mourning is important in offering holistic care to young adults and their caregivers, and points to the need for further research in this area.

Inequalities in neighbourhood socioeconomic characteristics: potential evidence-base for neighbourhood health planning

PubMed Central

Odoi, Agricola; Wray, Ron; Emo, Marion; Birch, Stephen; Hutchison, Brian; Eyles, John; Abernathy, Tom

2005-01-01

Background Population health planning aims to improve the health of the entire population and to reduce health inequities among population groups. Socioeconomic factors are increasingly being recognized as major determinants of many aspects of health and causes of health inequities. Knowledge of socioeconomic characteristics of neighbourhoods is necessary to identify their unique health needs and enhance identification of socioeconomically disadvantaged populations. Careful integration of this knowledge into health planning activities is necessary to ensure that health planning and service provision are tailored to unique neighbourhood population health needs. In this study, we identify unique neighbourhood socioeconomic characteristics and classify the neighbourhoods based on these characteristics. Principal components analysis (PCA) of 18 socioeconomic variables was used to identify the principal components explaining most of the variation in socioeconomic characteristics across the neighbourhoods. Cluster analysis was used to classify neighbourhoods based on their socioeconomic characteristics. Results Results of the PCA and cluster analysis were similar but the latter were more objective and easier to interpret. Five neighbourhood types with distinguishing socioeconomic and demographic characteristics were identified. The methodology provides a more complete picture of the neighbourhood socioeconomic characteristics than when a single variable (e.g. income) is used to classify neighbourhoods. Conclusion Cluster analysis is useful for generating neighbourhood population socioeconomic and demographic characteristics that can be useful in guiding neighbourhood health planning and service provision. This study is the first of a series of studies designed to investigate health inequalities at the neighbourhood level with a view to providing evidence-base for health planners, service providers and policy makers to help address health inequity issues at the neighbourhood level. Subsequent studies will investigate inequalities in health outcomes both within and across the neighbourhood types identified in the current study. PMID:16092969

Influence of Clinical Trial Site Enrollment on Patient Characteristics, Protocol Completion, and End Points: Insights From the ASCEND-HF Trial (Acute Study of Clinical Effectiveness of Nesiritide in Decompensated Heart Failure).

PubMed

Greene, Stephen J; Hernandez, Adrian F; Sun, Jie-Lena; Metra, Marco; Butler, Javed; Ambrosy, Andrew P; Ezekowitz, Justin A; Starling, Randall C; Teerlink, John R; Schulte, Phillip J; Voors, Adriaan A; Armstrong, Paul W; O'Connor, Christopher M; Mentz, Robert J

2016-09-01

Most international acute heart failure trials have failed to show benefit with respect to key end points. The impact of site enrollment and protocol execution on trial performance is unclear. We assessed the impact of varying site enrollment volume among all 7141 acute heart failure patients from the ASCEND-HF trial (Acute Study of Clinical Effectiveness of Nesiritide in Decompensated Heart Failure). Overall, 398 sites enrolled ≥1 patient, and median enrollment was 12 patients (interquartile range, 5-23). Patients from high enrolling sites (>60 patients/site) tended to have lower ejection fraction, worse New York Heart Association functional class, and lower utilization of guideline-directed medical therapy but fewer comorbidities and lower B-type natriuretic peptide level. Every 10 patient increase (up to 100 patients) in site enrollment correlated with lower likelihood of protocol noncompletion (odds ratio, 0.93; 95% confidence interval [CI], 0.89-0.98). After adjustment, increasing site enrollment predicted higher risk of persistent dyspnea at 6 hours (per 10 patient increase: odds ratio 1.02; 95% CI, 1.01-1.03) but not at 24 hours (odds ratio, 0.99; 95% CI, 0.98-1.00). Higher site enrollment was independently associated with lower risk of 30-day death or rehospitalization (per 10 patient increase: odds ratio, 0.98, 95% CI, 0.96-0.99) but not 180-day mortality (hazard ratio, 0.99; 95% CI, 0.98-1.01). The influence of increasing site enrollment on clinical end points varied across geographic regions with strongest associations in Latin America and Asia-Pacific (all interaction P<0.01). In this large, acute heart failure trial, site enrollment correlated with protocol completion and was independently associated with trial end points. Individual and regional site performance present challenges to be considered in design of future acute heart failure trials. URL: http://www.clinicaltrials.gov. Unique identifier: NCT00475852. © 2016 American Heart Association, Inc.

Trypanosome RNA polymerases and transcription factors: sensible trypanocidal drug targets?

PubMed

Vanhamme, Luc

2008-11-01

Trypanosomes and Leishmaniae are the agents of several important parasitic diseases threatening hundreds of million human beings worldwide. As they diverged early in evolution, they display original molecular characteristics. These peculiarities are each defining putative specific targets for anti-parasitic drugs. Transcription displays its lot of unique characteristics in trypanosomes and will be taken as an example to uncover these targets. Unique features of transcription in trypanosomes include constitutive and poly-cistronic transcription by RNA polymerase II as well as transcription of protein-coding genes by RNA polymerase I. It is becoming clear that these unique mechanisms are performed by dedicated molecular players. The first of them have been recently characterized. They are reviewed and their suitability as drug targets is commented.

One Course, One Web Site--Of Course? Maybe Not!

ERIC Educational Resources Information Center

Cohn, Ellen R.

2004-01-01

Colleges and universities increasingly employ commercial Web-based course management systems (such as Blackboard and WebCT). How is it, then, that these institutions unquestioningly allocate a unique Web site to each class? Why establish one Web site for one course when other options provide so many benefits? Why isn't there a clamor for…

Examining the Presence of Social Media on University Web Sites

ERIC Educational Resources Information Center

Greenwood, Grant

2012-01-01

Over the past few years, social networking has exploded into a massive medium that has captured the attention of a large portion of the American population. The ever-growing social networking site(s) (SNS) movement has filled a networking gap and thus, has presented higher education institutions with unique opportunities (Reid 2009) to further…

Multifocal multi-site Warthin tumour.

PubMed

Hilton, Jennifer M; Phillips, John S; Hellquist, Henrik B; Premachandra, Don J

2008-12-01

The unique case of a 55-year-old man with multifocal adenolymphoma (Warthin's tumour) of both parotid glands, the neck and post-nasal space is presented. Warthin tumour is almost exclusively a parotid tumour but is known to be bilateral in 7-10% of cases and multifocal in 2% of cases. Most extraglandular Warthin tumours have been located in neck lymph nodes and only a few cases have been reported from other sites. The presented case is unique in having synchronous and metachronous Warthin tumours, as well as one of the tumours being neither truly parotid, nor within a lymph node.

Newberry EGS Seismic Velocity Model

DOE Data Explorer

Templeton, Dennise

2013-10-01

We use ambient noise correlation (ANC) to create a detailed image of the subsurface seismic velocity at the Newberry EGS site down to 5 km. We collected continuous data for the 22 stations in the Newberry network, together with 12 additional stations from the nearby CC, UO and UW networks. The data were instrument corrected, whitened and converted to single bit traces before cross correlation according to the methodology in Benson (2007). There are 231 unique paths connecting the 22 stations of the Newberry network. The additional networks extended that to 402 unique paths crossing beneath the Newberry site.

Onco-Regulon: an integrated database and software suite for site specific targeting of transcription factors of cancer genes

PubMed Central

Tomar, Navneet; Mishra, Akhilesh; Mrinal, Nirotpal; Jayaram, B.

2016-01-01

Transcription factors (TFs) bind at multiple sites in the genome and regulate expression of many genes. Regulating TF binding in a gene specific manner remains a formidable challenge in drug discovery because the same binding motif may be present at multiple locations in the genome. Here, we present Onco-Regulon (http://www.scfbio-iitd.res.in/software/onco/NavSite/index.htm), an integrated database of regulatory motifs of cancer genes clubbed with Unique Sequence-Predictor (USP) a software suite that identifies unique sequences for each of these regulatory DNA motifs at the specified position in the genome. USP works by extending a given DNA motif, in 5′→3′, 3′ →5′ or both directions by adding one nucleotide at each step, and calculates the frequency of each extended motif in the genome by Frequency Counter programme. This step is iterated till the frequency of the extended motif becomes unity in the genome. Thus, for each given motif, we get three possible unique sequences. Closest Sequence Finder program predicts off-target drug binding in the genome. Inclusion of DNA-Protein structural information further makes Onco-Regulon a highly informative repository for gene specific drug development. We believe that Onco-Regulon will help researchers to design drugs which will bind to an exclusive site in the genome with no off-target effects, theoretically. Database URL: http://www.scfbio-iitd.res.in/software/onco/NavSite/index.htm PMID:27515825

[Ecological agriculture: future of agriculture for Chinese material medica].

PubMed

Guo, Lan-Ping; Wang, Tie-Lin; Yang, Wan-Zhen; Zhou, Liang-Yun; Chen, Nai-Fu; Han, Bang-Xing; Huang, Lu-Qi

2017-01-01

The ecological agriculture of traditional Chinese medicine (TCM) is generally acknowledged as the most advanced agricultural mode. However, it's still a doubt whether ecological agriculture could be widely applied in TCM agriculture. In this study, we first analyze both the differences and relationships between ecological and organic agriculture, which suggesting that ecological agriculture does not need all the inputs as traditional agriculture. After introducing the situation of ecological agriculture from all across the world, we analyze the differences and characteristics between ecological and chemical agricultures. Considered with the big challenge caused by chemical agriculture, we pointed out that ecological agriculture could definitely replace chemical agriculture. Last but not the least, combined with the situation and problems of Chinese agriculture, we analyze the distinctive advantages of TCM ecological agriculture from 3 aspects as its unique quality characteristics, its unique habitat requirements in production and its unique application and market characteristics, respectively. In conclusion, ecological agriculture is the straight way of TCM agriculture. Copyright© by the Chinese Pharmaceutical Association.

Site-characteristic and hydrologic data for selected wells and springs on Federal land in Clark County, Nevada

USGS Publications Warehouse

Pavelko, Michael T.

2014-01-01

Site-characteristic and hydrologic data for selected wells and springs on U.S. Bureau of Land Management, National Park Service, U.S. Fish and Wildlife Service, and U.S. Forest Service land in Clark County, Nevada, were updated in the U.S. Geological Survey’s National Water Information System (NWIS) to facilitate multi-agency research. Data were researched and reviewed, sites were visited, and NWIS data were updated for 231 wells and 198 springs, including 36 wells and 67 springs that were added to NWIS and 44 duplicate sites that were deleted. The site-characteristic and hydrologic data collected, reviewed, edited, and added to NWIS include locations, well water levels, spring discharges, and water chemistry. Site-characteristic and hydrologic data can be accessed from links to the NWIS web interface; data not available through the web interface are presented in appendixes to this report.

Design manual for low water stream crossings.

DOT National Transportation Integrated Search

1983-10-01

The purpose of this manual is to provide design guidelines for : low water stream crossings (LWSCs). Rigid criteria for determining the : applicability of a LWSC to a given site are not established since each : site is unique in terms of physical, so...

Assessment of Mars Exploration Rover Landing Site Predictions

NASA Technical Reports Server (NTRS)

Golombek, M. P.; Arvidson, R. E.; Bell, J. F., III; Christensen, P. R.; Crisp, J. A.; Ehlmann, B. L.; Fergason, R. L.; Grant, J. A.; Haldemann, A. F. C.; Parker, T. J.;

Crystal structure and characterization of a novel L-serine ammonia-lyase from Rhizomucor miehei

DOE Office of Scientific and Technical Information (OSTI.GOV)

Qin, Zhen; Yan, Qiaojuan; Ma, Qingjun

L-serine ammonia-lyase, as a member of the β-family of pyridoxal-5′-phosphate (PLP) dependent enzymes, catalyzes the conversion of L-serine (L-threonine) to pyruvate (α-ketobutyrate) and ammonia. The crystal structure of L-serine ammonia-lyase from Rhizomucor miehei (RmSDH) was solved at 1.76 Å resolution by X-ray diffraction method. The overall structure of RmSDH had the characteristic β-family PLP dependent enzyme fold. It consisted of two distinct domains, both of which show the typical open twisted α/β structure. A PLP cofactor was located in the crevice between the two domains, which was attached to Lys52 by a Schiff-base linkage. Unique residue substitutions (Gly78, Pro79, Ser146, Ser147more » and Thr312) were discovered at the catalytic site of RmSDH by comparison of structures of RmSDH and other reported eukaryotic L-serine ammonia-lyases. Optimal pH and temperature of the purified RmSDH were 7.5 and 40 °C, respectively. It was stable in the pH range of 7.0–9.0 and at temperatures below 40 °C. This is the first crystal structure of a fungal L-serine ammonia-lyase. It will be useful to study the catalytic mechanism of β-elimination enzymes and will provide a basis for further enzyme engineering. - Highlights: • The crystal structure of a fungal L-serine ammonia-lyase (RmSDH) was solved. • Five unique residue substitutions are found at the catalytic site of RmSDH. • RmSDH was expressed in Pichia. pastoris and biochemically characterized. • RmSDH has potential application in splitting D/L-serine.« less

Analysis of the Secretome and Identification of Novel Constituents from Culture Filtrate of Bacillus Calmette-Guérin Using High-resolution Mass Spectrometry*

PubMed Central

Zheng, Jianhua; Ren, Xianwen; Wei, Candong; Yang, Jian; Hu, Yongfeng; Liu, Liguo; Xu, Xingye; Wang, Jin; Jin, Qi

2013-01-01

Tuberculosis (TB) is an infectious bacterial disease that causes morbidity and mortality, especially in developing countries. Although its efficacy against TB has displayed a high degree of variability (0%–80%) in different trials, Mycobacterium bovis bacillus Calmette-Guérin (BCG) has been recognized as an important weapon for preventing TB worldwide for over 80 years. Because secreted proteins often play vital roles in the interaction between bacteria and host cells, the secretome of mycobacteria is considered to be an attractive reservoir of potential candidate antigens for the development of novel vaccines and diagnostic reagents. In this study, we performed a proteomic analysis of BCG culture filtrate proteins using SDS-PAGE and high-resolution Fourier transform mass spectrometry. In total, 239 proteins (1555 unique peptides) were identified, including 185 secreted proteins or lipoproteins. Furthermore, 17 novel protein products not annotated in the BCG database were detected and validated by means of RT-PCR at the transcriptional level. Additionally, the translational start sites of 52 proteins were confirmed, and 22 proteins were validated through extension of the translational start sites based on N-terminus-derived peptides. There are 103 secreted proteins that have not been reported in previous studies on the mycobacterial secretome and are unique to our study. The physicochemical characteristics of the secreted proteins were determined. Major components from the culture supernatant, including low-molecular-weight antigens, lipoproteins, Pro-Glu and Pro-Pro-Glu family proteins, and Mce family proteins, are discussed; some components represent potential predominant antigens in the humoral and cellular immune responses. PMID:23616670

Genetic and antigenic relationship of foot-and-mouth disease virus serotype O isolates with the vaccine strain O1/BFS.

PubMed

Xu, Wanhong; Zhang, Zhidong; Nfon, Charles; Yang, Ming

2018-05-15

Foot-and-mouth disease serotype O viruses (FMDV/O) are responsible for the most outbreaks in FMD endemic countries. O1/BFS is one of the recommended FMD/O vaccine strains by World Reference Laboratory for FMD. In the current study, FMDV/O1 BFS vaccine strain and serotype O field isolates (45) were analyzed phylogenetically and antigenically to gain more insight into the genetic and antigenic characteristics of the vaccine strain and field isolates. O1/BFS showed similarity with 89% of the field isolates using a virus neutralization test (VNT). The P1 region encoding the FMDV capsid was sequenced and analysed for 46 strains of FMDV/O. Phylogenetic analysis showed these viruses originated from five continents and covered eight of 11 reported topotypes. Five isolates that demonstrated low antigenic similarities with O1/BFS were analyzed for their antigenic variation at the known neutralizing antigenic sites. Three of the five isolates demonstrated unique amino acid substitutions at various antigenic sites. No unique amino acid substitutions were observed for the other two unmatched isolates. Positively selected residues were identified on the surface of the FMD virus capsid supporting that it is important to continuously monitor field isolates for their antigenic and phenotypic changes. In conclusion, the vaccine strain O1/BFS is likely to confer protection against 89% of the 45 FMDV/O isolates based on VNT. Thus O1/BFS vaccine strain is still suitable for use in global FMD serotype O outbreak control. Combining data from phylogenetic, molecular and antigenic analysis can provide improvements in the process of vaccine selection. Crown Copyright © 2018. Published by Elsevier Ltd. All rights reserved.

Characteristics and Effectiveness of the U.S. State E-Government-to-Business Services

ERIC Educational Resources Information Center

Zhao, Jensen J.; Truell, Allen; Alexander, Melody W.

2008-01-01

This study examined the user-interface characteristics and effectiveness of the e-government-to-business (G2B) sites of the 50 U.S. states and Washington, D.C. A group of 306 online users were trained to assess the sites. The findings indicate that the majority of the state G2B sites included the user-interface characteristics that provided online…

Signatures of Extended Storage of Used Nuclear Fuel in Casks

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rauch, Eric Benton

2016-09-28

As the amount of used nuclear fuel continues to grow, more and more used nuclear fuel will be transferred to storage casks. A consolidated storage facility is currently in the planning stages for storing these casks, where at least 10,000 MTHM of fuel will be stored. This site will have potentially thousands of casks once it is operational. A facility this large presents new safeguards and nuclear material accounting concerns. A new signature based on the distribution of neutron sources and multiplication within casks was part of the Department of Energy Office of Nuclear Energy’s Material Protection, Account and Controlmore » Technologies (MPACT) campaign. Under this project we looked at fingerprinting each cask's neutron signature. Each cask has a unique set of fuel, with a unique spread of initial enrichment, burnup, cooling time, and power history. The unique set of fuel creates a unique signature of neutron intensity based on the arrangement of the assemblies. The unique arrangement of neutron sources and multiplication produces a reliable and unique identification of the cask that has been shown to be relatively constant over long time periods. The work presented here could be used to restore from a loss of continuity of knowledge at the storage site. This presentation will show the steps used to simulate and form this signature from the start of the effort through its conclusion in September 2016.« less

Post-Closure Challenges of U.S. Department of Energy Sites in Desert Environments of the Southwestern United States - 12095

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gil, April; Steckley, Deborah; Gauthier, Cassie

2012-07-01

U.S. Department of Energy (DOE) sites located in harsh desert environments of the Four Corners region of the southwestern United States require diligence and continual maintenance to ensure the remediation systems function as designed to protect human health and the environment. The geology and climate of this area create issues that are unique to these sites. Geologic formations contain naturally occurring constituents that are often the same as the residual contaminants remaining from historical milling activities at the sites. Although annual precipitation is low, when precipitation events occur they can be of extreme intensity, resulting in erosion and flooding thatmore » can quickly destroy infrastructure and rapidly change site conditions. Winds can cause sand storms and sand mounding that effect site features. These challenging environmental conditions, along with the remote locations of the sites, require active management beyond what was originally envisioned for uranium disposal sites to address concerns in a safe and cost-effective manner. The unique environment of the Four Corners region creates many challenges to the LTSM of LM sites in southwestern United States. The remediation efforts and approaches to infrastructure have to be specifically structured to work in this environment. Often, the systems and structures have to be modified based on lessons learned on how to best adapt to these difficult conditions and remote locations. These sites require continual maintenance and additional efforts compared to many other LM sites. (authors)« less

Baseline report - tall upland shrubland at the Rocky Flats Environmental Technology Site

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

Rocky Flats Environmental Technology Site (Site) is located on the Colorado Piedmont east of the Front Range between Boulder and Golden. At an elevation of approximately 6,000 feet, the Site contains a unique ecotonal mixture of mountain and prairie plant species, resulting from the topography and close proximity to the mountain front. The Buffer Zone surrounding the Industrial Area is one of the largest remaining undeveloped areas of its kind along the Colorado Piedmont. A number of plant communities at the Site have been identified as increasingly rare and unique by Site ecologists and the Colorado Natural Heritage Program (CNHP).more » These include the xeric tallgrass prairie, tall upland shrubland, wetlands, and Great Plains riparian woodland communities. Many of these communities support populations of increasingly rare animals as well, including the Preble`s meadow jumping mouse, grasshopper sparrow, loggerhead shrike, Merriam`s shrew, black crowned night heron, and Hops blue and Argos skipper butterflies. One of the more interesting and important plant communities at the Site is the tall upland shrubland community. It has been generally overlooked by previous Site ecological studies, probably due to its relatively small size; only 34 acres total. Although mentioned in a plant community ordination study conducted by Clark et al. and also in the Site baseline ecological study, few data were available on this plant community before the present study.« less

Population-specific recombination sites within the human MHC region.

PubMed

Lam, T H; Shen, M; Chia, J-M; Chan, S H; Ren, E C

2013-08-01

Genetic rearrangement by recombination is one of the major driving forces for genome evolution, and recombination is known to occur in non-random, discreet recombination sites within the genome. Mapping of recombination sites has proved to be difficult, particularly, in the human MHC region that is complicated by both population variation and highly polymorphic HLA genes. To overcome these problems, HLA-typed individuals from three representative populations: Asian, European and African were used to generate phased HLA haplotypes. Extended haplotype homozygosity (EHH) plots constructed from the phased haplotype data revealed discreet EHH drops corresponding to recombination events and these signatures were observed to be different for each population. Surprisingly, the majority of recombination sites detected are unique to each population, rather than being common. Unique recombination sites account for 56.8% (21/37 of total sites) in the Asian cohort, 50.0% (15/30 sites) in Europeans and 63.2% (24/38 sites) in Africans. Validation carried out at a known sperm typing recombination site of 45 kb (HLA-F-telomeric) showed that EHH was an efficient method to narrow the recombination region to 826 bp, and this was further refined to 660 bp by resequencing. This approach significantly enhanced mapping of the genomic architecture within the human MHC, and will be useful in studies to identify disease risk genes.

Coordination Environment of a Site-Bound Metal Ion in the Hammerhead Ribozyme Determined by 15N and 2H ESEEM Spectroscopy

PubMed Central

Vogt, Matthew; Lahiri, Simanti; Hoogstraten, Charles G.; Britt, R. David; DeRose, Victoria J.

2010-01-01

Although site-bound Mg2+ ions have been proposed to influence RNA structure and function, establishing the molecular properties of such sites has been challenging due largely to the unique electrostatic properties of the RNA biopolymer. We have previously determined that, in solution, the hammerhead ribozyme (a self-cleaving RNA) has a high-affinity metal ion binding site characterized by a Kd,app < 10 µM for Mn2+ in 1 M NaCl and speculated that this site has functional importance in the ribozyme cleavage reaction. Here we determine both the precise location and the hydration level of Mn2+ in this site using ESEEM (electron spin–echo envelope modulation) spectroscopy. Definitive assignment of the high-affinity site to the activity-sensitive A9/G10.1 region is achieved by site-specific labeling of G10.1 with 15N guanine. The coordinated metal ion retains four water ligands as measured by 2H ESEEM spectroscopy. The results presented here show that a functionally important, specific metal binding site is uniquely populated in the hammerhead ribozyme even in a background of high ionic strength. Although it has a relatively high thermodynamic affinity, this ion remains partially hydrated and is chelated to the RNA by just two ligands. PMID:17177426

SOLVENT EXTRACTION PROCESSES: A SURVEY OF SYSTEMS IN THE SITE PROGRAM

EPA Science Inventory

Solvent extraction of contaminated soils, sludges and sediments has been successfully completed at a number ofSuperfund sites. Each commercialized process uses a unique operating system to extract organic contaminants from solids. These operating systems may be classified by the ...

Habitat characteristics of larval mosquitoes in zoos of South Carolina, USA.

PubMed

Tuten, Holly C

2011-06-01

To investigate whether the unique assemblage of habitats in zoos could affect mosquito oviposition behavior and to provide zoos with suggestions for mosquito control, larvae were sampled and associated habitat variables were measured in 2 zoos in South Carolina, U.S.A. Fifty-nine sites were sampled from March 2008 to January 2009. A total of 1630 larvae representing 16 species was collected and identified. The dominant species was Aedes albopictus (46.0%), followed by Ae. triseriatus (23.6%), Culex restuans (12.4%), and Cx. pipiens complex (9.7%). Principal components and multiple logistic regression analyses showed that across both zoos the distribution of Ae. albopictus larvae was predicted by ambient and site temperature, precipitation, dissolved oxygen, and container habitats. The distribution of Ae. triseriatus larvae was predicted by natural containers and shade height < or =2 m. Overall larval mosquito presence (regardless of species) was predicted by ambient and site temperature, precipitation, dissolved oxygen, presence of natural habitats, and absence of aquatic vegetation. Additionally, C8 values of pairwise species associations indicated significant habitat-based relationships between Ae. albopictus and Ae. triseriatus, and Cx. pipiens complex and Cx. restuans. In general, species-habitat associations conformed to previously published studies. Recommendations to zoo personnel include elimination of artificial container habitats, reduction of shade sources < or =2 m over aquatic habitats, use of approved mosquito larvicides, and training in recognizing and mitigating larval mosquito habitats.

Desert bighorn sheep lambing habitat: Parturition, nursery, and predation sites

USGS Publications Warehouse

Karsch, Rebekah C.; Cain, James W.; Rominger, Eric M.; Goldstein, Elise J.

2016-01-01

Fitness of female ungulates is determined by neonate survival and lifetime reproductive success. Therefore, adult female ungulates should adopt behaviors and habitat selection patterns that enhance survival of neonates during parturition and lactation. Parturition site location may play an important role in neonatal mortality of desert bighorn sheep (Ovis canadensis mexicana) when lambs are especially vulnerable to predation, but parturition sites are rarely documented for this species. Our objectives were to assess environmental characteristics at desert bighorn parturition, lamb nursery, and predation sites and to assess differences in habitat characteristics between parturition sites and nursery group sites, and predation sites and nursery group sites. We used vaginal implant transmitters (VITs) to identify parturition sites and capture neonates. We then compared elevation, slope, terrain ruggedness, and visibility at parturition, nursery, and lamb predation sites with paired random sites and compared characteristics of parturition sites and lamb predation sites to those of nursery sites. When compared to random sites, odds of a site being a parturition site were highest at intermediate slopes and decreased with increasing female visibility. Odds of a site being a predation site increased with decreasing visibility. When compared to nursery group sites, odds of a site being a parturition site had a quadratic relationship with elevation and slope, with odds being highest at intermediate elevations and intermediate slopes. When we compared predation sites to nursery sites, odds of a site being a predation were highest at low elevation areas with high visibility and high elevation areas with low visibility likely because of differences in hunting strategies of coyote (Canis latrans) and puma (Puma concolor). Parturition sites were lower in elevation and slope than nursery sites. Understanding selection of parturition sites by adult females and how habitat characteristics at these sites differ from those at predation and nursery sites can provide insight into strategies employed by female desert bighorn sheep and other species during and after parturition to promote neonate survival.

sc-PDB: a 3D-database of ligandable binding sites—10 years on

PubMed Central

Desaphy, Jérémy; Bret, Guillaume; Rognan, Didier; Kellenberger, Esther

2015-01-01

The sc-PDB database (available at http://bioinfo-pharma.u-strasbg.fr/scPDB/) is a comprehensive and up-to-date selection of ligandable binding sites of the Protein Data Bank. Sites are defined from complexes between a protein and a pharmacological ligand. The database provides the all-atom description of the protein, its ligand, their binding site and their binding mode. Currently, the sc-PDB archive registers 9283 binding sites from 3678 unique proteins and 5608 unique ligands. The sc-PDB database was publicly launched in 2004 with the aim of providing structure files suitable for computational approaches to drug design, such as docking. During the last 10 years we have improved and standardized the processes for (i) identifying binding sites, (ii) correcting structures, (iii) annotating protein function and ligand properties and (iv) characterizing their binding mode. This paper presents the latest enhancements in the database, specifically pertaining to the representation of molecular interaction and to the similarity between ligand/protein binding patterns. The new website puts emphasis in pictorial analysis of data. PMID:25300483

Discovery of a small-molecule HIV-1 integrase inhibitor-binding site | Center for Cancer Research

Cancer.gov

The lowest energy-binding conformation of an inhibitor bound to the dimeric interface of HIV-1 integrase core domain. The yellow region represents a unique allosteric binding site identified by affinity labeling and mass spectrometry and validated through mutagenesis. This site can provide a potential platform for the rational design of inhibitors selective for disruption of

sc-PDB: a 3D-database of ligandable binding sites--10 years on.

PubMed

Desaphy, Jérémy; Bret, Guillaume; Rognan, Didier; Kellenberger, Esther

2015-01-01

The sc-PDB database (available at http://bioinfo-pharma.u-strasbg.fr/scPDB/) is a comprehensive and up-to-date selection of ligandable binding sites of the Protein Data Bank. Sites are defined from complexes between a protein and a pharmacological ligand. The database provides the all-atom description of the protein, its ligand, their binding site and their binding mode. Currently, the sc-PDB archive registers 9283 binding sites from 3678 unique proteins and 5608 unique ligands. The sc-PDB database was publicly launched in 2004 with the aim of providing structure files suitable for computational approaches to drug design, such as docking. During the last 10 years we have improved and standardized the processes for (i) identifying binding sites, (ii) correcting structures, (iii) annotating protein function and ligand properties and (iv) characterizing their binding mode. This paper presents the latest enhancements in the database, specifically pertaining to the representation of molecular interaction and to the similarity between ligand/protein binding patterns. The new website puts emphasis in pictorial analysis of data. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

Structure-Function Studies with the Unique Hexameric Form II Ribulose-1,5-bisphosphate Carboxylase/Oxygenase (Rubisco) from Rhodopseudomonas palustris*

PubMed Central

Satagopan, Sriram; Chan, Sum; Perry, L. Jeanne; Tabita, F. Robert

2014-01-01

The first x-ray crystal structure has been solved for an activated transition-state analog-bound form II ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). This enzyme, from Rhodopseudomonas palustris, assembles as a unique hexamer with three pairs of catalytic large subunit homodimers around a central 3-fold symmetry axis. This oligomer arrangement is unique among all known Rubisco structures, including the form II homolog from Rhodospirillum rubrum. The presence of a transition-state analog in the active site locked the activated enzyme in a “closed” conformation and revealed the positions of critical active site residues during catalysis. Functional roles of two form II-specific residues (Ile165 and Met331) near the active site were examined via site-directed mutagenesis. Substitutions at these residues affect function but not the ability of the enzyme to assemble. Random mutagenesis and suppressor selection in a Rubisco deletion strain of Rhodobacter capsulatus identified a residue in the amino terminus of one subunit (Ala47) that compensated for a negative change near the active site of a neighboring subunit. In addition, substitution of the native carboxyl-terminal sequence with the last few dissimilar residues from the related R. rubrum homolog increased the enzyme's kcat for carboxylation. However, replacement of a longer carboxyl-terminal sequence with termini from either a form III or a form I enzyme, which varied both in length and sequence, resulted in complete loss of function. From these studies, it is evident that a number of subtle interactions near the active site and the carboxyl terminus account for functional differences between the different forms of Rubiscos found in nature. PMID:24942737

Structure-function studies with the unique hexameric form II ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from Rhodopseudomonas palustris.

PubMed

Satagopan, Sriram; Chan, Sum; Perry, L Jeanne; Tabita, F Robert

2014-08-01

The first x-ray crystal structure has been solved for an activated transition-state analog-bound form II ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). This enzyme, from Rhodopseudomonas palustris, assembles as a unique hexamer with three pairs of catalytic large subunit homodimers around a central 3-fold symmetry axis. This oligomer arrangement is unique among all known Rubisco structures, including the form II homolog from Rhodospirillum rubrum. The presence of a transition-state analog in the active site locked the activated enzyme in a "closed" conformation and revealed the positions of critical active site residues during catalysis. Functional roles of two form II-specific residues (Ile(165) and Met(331)) near the active site were examined via site-directed mutagenesis. Substitutions at these residues affect function but not the ability of the enzyme to assemble. Random mutagenesis and suppressor selection in a Rubisco deletion strain of Rhodobacter capsulatus identified a residue in the amino terminus of one subunit (Ala(47)) that compensated for a negative change near the active site of a neighboring subunit. In addition, substitution of the native carboxyl-terminal sequence with the last few dissimilar residues from the related R. rubrum homolog increased the enzyme's kcat for carboxylation. However, replacement of a longer carboxyl-terminal sequence with termini from either a form III or a form I enzyme, which varied both in length and sequence, resulted in complete loss of function. From these studies, it is evident that a number of subtle interactions near the active site and the carboxyl terminus account for functional differences between the different forms of Rubiscos found in nature. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

How spatio-temporal habitat connectivity affects amphibian genetic structure.

PubMed

Watts, Alexander G; Schlichting, Peter E; Billerman, Shawn M; Jesmer, Brett R; Micheletti, Steven; Fortin, Marie-Josée; Funk, W Chris; Hapeman, Paul; Muths, Erin; Murphy, Melanie A

2015-01-01

Heterogeneous landscapes and fluctuating environmental conditions can affect species dispersal, population genetics, and genetic structure, yet understanding how biotic and abiotic factors affect population dynamics in a fluctuating environment is critical for species management. We evaluated how spatio-temporal habitat connectivity influences dispersal and genetic structure in a population of boreal chorus frogs (Pseudacris maculata) using a landscape genetics approach. We developed gravity models to assess the contribution of various factors to the observed genetic distance as a measure of functional connectivity. We selected (a) wetland (within-site) and (b) landscape matrix (between-site) characteristics; and (c) wetland connectivity metrics using a unique methodology. Specifically, we developed three networks that quantify wetland connectivity based on: (i) P. maculata dispersal ability, (ii) temporal variation in wetland quality, and (iii) contribution of wetland stepping-stones to frog dispersal. We examined 18 wetlands in Colorado, and quantified 12 microsatellite loci from 322 individual frogs. We found that genetic connectivity was related to topographic complexity, within- and between-wetland differences in moisture, and wetland functional connectivity as contributed by stepping-stone wetlands. Our results highlight the role that dynamic environmental factors have on dispersal-limited species and illustrate how complex asynchronous interactions contribute to the structure of spatially-explicit metapopulations.

Comparing scat detection dogs, cameras, and hair snares for surveying carnivores

USGS Publications Warehouse

Long, Robert A.; Donovan, T.M.; MacKay, Paula; Zielinski, William J.; Buzas, Jeffrey S.

2007-01-01

Carnivores typically require large areas of habitat, exist at low natural densities, and exhibit elusive behavior - characteristics that render them difficult to study. Noninvasive survey methods increasingly provide means to collect extensive data on carnivore occupancy, distribution, and abundance. During the summers of 2003-2004, we compared the abilities of scat detection dogs, remote cameras, and hair snares to detect black bears (Ursus americanus), fishers (Martes pennanti), and bobcats (Lynx rufus) at 168 sites throughout Vermont. All 3 methods detected black bears; neither fishers nor bobcats were detected by hair snares. Scat detection dogs yielded the highest raw detection rate and probability of detection (given presence) for each of the target species, as well as the greatest number of unique detections (i.e., occasions when only one method detected the target species). We estimated that the mean probability of detecting the target species during a single visit to a site with a detection dog was 0.87 for black bears, 0.84 for fishers, and 0.27 for bobcats. Although the cost of surveying with detection dogs was higher than that of remote cameras or hair snares, the efficiency of this method rendered it the most cost-effective survey method.

Post-invasion demography of prehistoric humans in South America.

PubMed

Goldberg, Amy; Mychajliw, Alexis M; Hadly, Elizabeth A

2016-04-14

As the last habitable continent colonized by humans, the site of multiple domestication hotspots, and the location of the largest Pleistocene megafaunal extinction, South America is central to human prehistory. Yet remarkably little is known about human population dynamics during colonization, subsequent expansions, and domestication. Here we reconstruct the spatiotemporal patterns of human population growth in South America using a newly aggregated database of 1,147 archaeological sites and 5,464 calibrated radiocarbon dates spanning fourteen thousand to two thousand years ago (ka). We demonstrate that, rather than a steady exponential expansion, the demographic history of South Americans is characterized by two distinct phases. First, humans spread rapidly throughout the continent, but remained at low population sizes for 8,000 years, including a 4,000-year period of 'boom-and-bust' oscillations with no net growth. Supplementation of hunting with domesticated crops and animals had a minimal impact on population carrying capacity. Only with widespread sedentism, beginning ~5 ka, did a second demographic phase begin, with evidence for exponential population growth in cultural hotspots, characteristic of the Neolithic transition worldwide. The unique extent of humanity's ability to modify its environment to markedly increase carrying capacity in South America is therefore an unexpectedly recent phenomenon.

Sensitive and Structure-Informative N-Glycosylation Analysis by MALDI-MS; Ionization, Fragmentation, and Derivatization

PubMed Central

Nishikaze, Takashi

2017-01-01

Mass spectrometry (MS) has become an indispensable tool for analyzing post translational modifications of proteins, including N-glycosylated molecules. Because most glycosylation sites carry a multitude of glycans, referred to as “glycoforms,” the purpose of an N-glycosylation analysis is glycoform profiling and glycosylation site mapping. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has unique characteristics that are suited for the sensitive analysis of N-glycosylated products. However, the analysis is often hampered by the inherent physico-chemical properties of N-glycans. Glycans are highly hydrophilic in nature, and therefore tend to show low ion yields in both positive- and negative-ion modes. The labile nature and complicated branched structures involving various linkage isomers make structural characterization difficult. This review focuses on MALDI-MS-based approaches for enhancing analytical performance in N-glycosylation research. In particular, the following three topics are emphasized: (1) Labeling for enhancing the ion yields of glycans and glycopeptides, (2) Negative-ion fragmentation for less ambiguous elucidation of the branched structure of N-glycans, (3) Derivatization for the stabilization and linkage isomer discrimination of sialic acid residues. PMID:28794918

Hidden in plain sight: a crowdsourced public art contest to make automated external defibrillators more visible.

PubMed

Merchant, Raina M; Griffis, Heather M; Ha, Yoonhee P; Kilaru, Austin S; Sellers, Allison M; Hershey, John C; Hill, Shawndra S; Kramer-Golinkoff, Emily; Nadkarni, Lindsay; Debski, Margaret M; Padrez, Kevin A; Becker, Lance B; Asch, David A

2014-12-01

We sought to explore the feasibility of using a crowdsourcing study to promote awareness about automated external defibrillators (AEDs) and their locations. The Defibrillator Design Challenge was an online initiative that asked the public to create educational designs that would enhance AED visibility, which took place over 8 weeks, from February 6, 2014, to April 6, 2014. Participants were encouraged to vote for AED designs and share designs on social media for points. Using a mixed-methods study design, we measured participant demographics and motivations, design characteristics, dissemination, and Web site engagement. Over 8 weeks, there were 13 992 unique Web site visitors; 119 submitted designs and 2140 voted. The designs were shared 48 254 times on Facebook and Twitter. Most designers-voters reported that they participated to contribute to an important cause (44%) rather than to win money (0.8%). Design themes included: empowerment, location awareness, objects (e.g., wings, lightning, batteries, lifebuoys), and others. The Defibrillator Design Challenge engaged a broad audience to generate AED designs and foster awareness. This project provides a framework for using design and contest architecture to promote health messages.

Hidden in Plain Sight: A Crowdsourced Public Art Contest to Make Automated External Defibrillators More Visible

PubMed Central

Griffis, Heather M.; Kilaru, Austin S.; Sellers, Allison M.; Hershey, John C.; Hill, Shawndra S.; Kramer-Golinkoff, Emily; Nadkarni, Lindsay; Debski, Margaret M.; Padrez, Kevin A.; Becker, Lance B.; Asch, David A.

2014-01-01

Objectives. We sought to explore the feasibility of using a crowdsourcing study to promote awareness about automated external defibrillators (AEDs) and their locations. Methods. The Defibrillator Design Challenge was an online initiative that asked the public to create educational designs that would enhance AED visibility, which took place over 8 weeks, from February 6, 2014, to April 6, 2014. Participants were encouraged to vote for AED designs and share designs on social media for points. Using a mixed-methods study design, we measured participant demographics and motivations, design characteristics, dissemination, and Web site engagement. Results. Over 8 weeks, there were 13 992 unique Web site visitors; 119 submitted designs and 2140 voted. The designs were shared 48 254 times on Facebook and Twitter. Most designers–voters reported that they participated to contribute to an important cause (44%) rather than to win money (0.8%). Design themes included: empowerment, location awareness, objects (e.g., wings, lightning, batteries, lifebuoys), and others. Conclusions. The Defibrillator Design Challenge engaged a broad audience to generate AED designs and foster awareness. This project provides a framework for using design and contest architecture to promote health messages. PMID:25320902

Partial reconstitution of photoreceptor cGMP phosphodiesterase characteristics in cGMP phosphodiesterase-5.

PubMed

Granovsky, A E; Artemyev, N O

2001-06-15

Photoreceptor cGMP phosphodiesterases (PDE6) are uniquely qualified to serve as effector enzymes in the vertebrate visual transduction cascade. In the dark-adapted photoreceptors, the activity of PDE6 is blocked via tight association with the inhibitory gamma-subunits (Pgamma). The Pgamma block is removed in the light-activated PDE6 by the visual G protein, transducin. Transducin-activated PDE6 exhibits an exceptionally high catalytic rate of cGMP hydrolysis ensuring high signal amplification. To identify the structural determinants for the inhibitory interaction with Pgamma and the remarkable cGMP hydrolytic ability, we sought to reproduce the PDE6 characteristics by mutagenesis of PDE5, a related cyclic GMP-specific, cGMP-binding PDE. PDE5 is insensitive to Pgamma and has a more than 100-fold lower k(cat) for cGMP hydrolysis. Our mutational analysis of chimeric PDE5/PDE6alpha' enzymes revealed that the inhibitory interaction of cone PDE6 catalytic subunits (PDE6alpha') with Pgamma is mediated primarily by three hydrophobic residues at the entry to the catalytic pocket, Met(758), Phe(777), and Phe(781). The maximal catalytic rate of PDE5 was enhanced by at least 10-fold with substitutions of PDE6alpha'-specific glycine residues for the corresponding PDE5 alanine residues, Ala(608) and Ala(612). The Gly residues are adjacent to the highly conserved metal binding motif His-Asn-X-X-His, which is essential for cGMP hydrolysis. Our results suggest that the unique Gly residues allow the PDE6 metal binding site to adopt a more favorable conformation for cGMP hydrolysis.

Characteristics of American marten den sites in Wyoming

Treesearch

Leonard F. Ruggiero; Dean E. Pearson; Stephen E. Henry

1998-01-01

We examined characteristics of den structures and den sites used by female American marten (Martes americana) for natal and maternal dens in the Sierra Madre Range, Wyoming. During 1988-95, we located 18 natal dens (parturition sites) and 97 maternal dens (sites where kits were present exclusive of parturition) used by 10 female marten. Important den structures...

Unique Features of Halophilic Proteins.

PubMed

Arakawa, Tsutomu; Yamaguchi, Rui; Tokunaga, Hiroko; Tokunaga, Masao

2017-01-01

Proteins from moderate and extreme halophiles have unique characteristics. They are highly acidic and hydrophilic, similar to intrinsically disordered proteins. These characteristics make the halophilic proteins soluble in water and fold reversibly. In addition to reversible folding, the rate of refolding of halophilic proteins from denatured structure is generally slow, often taking several days, for example, for extremely halophilic proteins. This slow folding rate makes the halophilic proteins a novel model system for folding mechanism analysis. High solubility and reversible folding also make the halophilic proteins excellent fusion partners for soluble expression of recombinant proteins.

The Genomic Evolution of Prostate Cancer

DTIC Science & Technology

2014-10-01

Mutation characteristics. (a) Number of high-confidence somatic mutations across all foci. Non- silent , non- silent mutations; Unique, number of unique...genes harboring a non- silent mutation; Reported, gene reported to be mutated in references 9–12 and 14. (b) Spectrum of unique high confidence somatic...epigenetic and micr- oRNA-mediated inactivation of LRP1B, a modulator of the extracellular environment of thyroid cancer cells. Oncogene 2011; 30

Methods for estimating streamflow characteristics at ungaged sites in western Montana based on data through water year 2009: Chapter G in Montana StreamStats

USGS Publications Warehouse

McCarthy, Peter M.; Sando, Roy; Sando, Steven K.; Dutton, DeAnn M.

2016-04-05

All of the data used to calculate basin characteristics were derived from publicly available data sources and are available through the U.S. Geological Survey Streamstats program (http://water.usgs.gov/osw/streamstats/) for Montana. The primary purpose of the Montana StreamStats application is to provide estimates of basin characteristics and streamflow characteristics for user-selected ungaged sites on Montana streams. The regional regression equations presented in this report have been loaded to the Montana StreamStats application and can be used to derive streamflow characteristics for ungaged sites.

Arsenic Adsorption and As (III) Oxidation on TiO2 Nanoparticles: Macroscopic and Spectroscopic Investigations

EPA Science Inventory

Engineered nanoparticles (NPs) (particle sizes ranging from 1-100 nm) have unique physical and chemical properties that differ fundamentally from their macro-sized counterparts. In addition to their smaller particle size, nanoparticles possess unique characteristics such as larg...

Strategies for Increasing Academic Achievement in Higher Education

ERIC Educational Resources Information Center

Ensign, Julene; Woods, Amelia Mays

2014-01-01

Higher education today faces unique challenges. Decreasing student engagement, increasing diversity, and limited resources all contribute to the issues being faced by students, educators, and administrators alike. The unique characteristics and expectations that students bring to their professional programs require new methods of addressing…

What determines the relationship between color naming, unique hues, and sensory singularities: Illuminations, surfaces, or photoreceptors?

PubMed

Witzel, Christoph; Cinotti, François; O'Regan, J Kevin

2015-01-01

The relationship between the sensory signal of the photoreceptors on one hand and color appearance and language on the other hand is completely unclear. A recent finding established a surprisingly accurate correlation between focal colors, unique hues, and so-called singularities in the laws governing how sensory signals for different surfaces change across illuminations. This article examines how this correlation with singularities depends on reflectances, illuminants, and cone sensitivities. Results show that this correlation holds for a large range of illuminants and for a large range of sensors, including sensors that are fundamentally different from human photoreceptors. In contrast, the spectral characteristics of the reflectance spectra turned out to be the key factor that determines the correlation between focal colors, unique hues, and sensory singularities. These findings suggest that the origins of color appearance and color language may be found in particular characteristics of the reflectance spectra that correspond to focal colors and unique hues.

Nest Site Characteristics of Cavity Nesting Birds in Central Missouri

Treesearch

Jeffery D. Brawn; Bernice Tannenbaum; Keith E. Evans

1984-01-01

Two study sites in central Missouri oak-hickory forests were searched for nest sites of cavity nesting birds. Researchers located and measured 133 nests of 11 species. Cavity nesting bird habitat selection is affected by both snag characteristics and vegetation structure.

Study on the Antimicrobial Properties of Citrate-Based Biodegradable Polymers

PubMed Central

Su, Lee-Chun; Xie, Zhiwei; Zhang, Yi; Nguyen, Kytai Truong; Yang, Jian

2014-01-01

Citrate-based polymers possess unique advantages for various biomedical applications since citric acid is a natural metabolism product, which is biocompatible and antimicrobial. In polymer synthesis, citric acid also provides multiple functional groups to control the crosslinking of polymers and active binding sites for further conjugation of biomolecules. Our group recently developed a number of citrate-based polymers for various biomedical applications by taking advantage of their controllable chemical, mechanical, and biological characteristics. In this study, various citric acid derived biodegradable polymers were synthesized and investigated for their physicochemical and antimicrobial properties. Results indicate that citric acid derived polymers reduced bacterial proliferation to different degrees based on their chemical composition. Among the studied polymers, poly(octamethylene citrate) showed ~70–80% suppression to microbe proliferation, owing to its relatively higher ratio of citric acid contents. Crosslinked urethane-doped polyester elastomers and biodegradable photoluminescent polymers also exhibited significant bacteria reduction of ~20 and ~50% for Staphylococcus aureus and Escherichia coli, respectively. Thus, the intrinsic antibacterial properties in citrate-based polymers enable them to inhibit bacteria growth without incorporation of antibiotics, silver nanoparticles, and other traditional bacteria-killing agents suggesting that the citrate-based polymers are unique beneficial materials for wound dressing, tissue engineering, and other potential medical applications where antimicrobial property is desired. PMID:25023605

Cell-free unnatural amino acid incorporation with alternative energy systems and linear expression templates.

PubMed

Shrestha, Prashanta; Smith, Mark Thomas; Bundy, Bradley Charles

2014-01-25

Site-specific incorporation of unnatural amino acids (uAAs) during protein synthesis expands the proteomic code through the addition of unique residue chemistry. This field provides a unique tool to improve pharmacokinetics, cancer treatments, vaccine development, proteomics and protein engineering. The limited ability to predict the characteristics of proteins with uAA-incorporation creates a need for a low-cost system with the potential for rapid screening. Escherichia coli-based cell-free protein synthesis is a compelling platform for uAA incorporation due to the open and accessible nature of the reaction environment. However, typical cell-free systems can be expensive due to the high cost of energizing reagents. By employing alternative energy sources, we reduce the cost of uAA-incorporation in CFPS by 55%. While alternative energy systems reduce cost, the time investment to develop gene libraries can remain cumbersome. Cell-free systems allow the direct use of PCR products known as linear expression templates, thus alleviating tedious plasmid library preparations steps. We report the specific costs of CFPS with uAA incorporation, demonstrate that LETs are suitable expression templates with uAA-incorporation, and consider the substantial reduction in labor intensity using LET-based expression for CFPS uAA incorporation. Copyright © 2013 Elsevier B.V. All rights reserved.

A multi-case study of school gardens in Southwest Montana

NASA Astrophysics Data System (ADS)

Yates, Kimberly Ann

Many children today spend little time outdoors exploring the natural world and a great deal of time inside viewing the television or playing video games. This new condition of childhood has many negative ramifications, such as poor social development, childhood obesity, and a lack of feeling connected to the environment. One instructional tool being used by some schools to address these rising concerns is a school garden. School gardens can provide an opportunity for students to experience learning in a real-world application, outside of the classroom walls. This qualitative multi-case study explores three school gardens in Southwest Montana and tells each of their unique stories. Through the process of participant observation, interviews, and the collection of multiple data sources, a thorough description is given of the history behind the gardens, how they have impacted the teachers and students, what challenges they have faced, and the common characteristics found in a successful school garden program. During the data analysis process, themes for each case study site were revealed. The results of this study found that each school garden was unique in character and purpose and that a number of dedicated garden supporters are essential to the success of a garden program. In conclusion, suggestions and resources were provided for practitioners interested in pursuing a garden program.

The Coevolution of Life and Environment on Mars: An Ecosystem Perspective on the Robotic Exploration of Biosignatures

NASA Astrophysics Data System (ADS)

Cabrol, Nathalie A.

2018-01-01

Earth's biological and environmental evolution are intertwined and inseparable. This coevolution has become a fundamental concept in astrobiology and is key to the search for life beyond our planet. In the case of Mars, whether a coevolution took place is unknown, but analyzing the factors at play shows the uniqueness of each planetary experiment regardless of similarities. Early Earth and early Mars shared traits. However, biological processes on Mars, if any, would have had to proceed within the distinctive context of an irreversible atmospheric collapse, greater climate variability, and specific planetary characteristics. In that, Mars is an important test bed for comparing the effects of a unique set of spatiotemporal changes on an Earth-like, yet different, planet. Many questions remain unanswered about Mars' early environment. Nevertheless, existing data sets provide a foundation for an intellectual framework where notional coevolution models can be explored. In this framework, the focus is shifted from planetary-scale habitability to the prospect of habitats, microbial ecotones, pathways to biological dispersal, biomass repositories, and their meaning for exploration. Critically, as we search for biosignatures, this focus demonstrates the importance of starting to think of early Mars as a biosphere and vigorously integrating an ecosystem approach to landing site selection and exploration.

Anaerobic Ammonium-Oxidizing Bacteria: Unique Microorganisms with Exceptional Properties

PubMed Central

Jetten, Mike S. M.

2012-01-01

Summary: Anaerobic ammonium-oxidizing (anammox) bacteria defy many microbiological concepts and share numerous properties with both eukaryotes and archaea. Among their most intriguing characteristics are their compartmentalized cell plan and archaeon-like cell wall. Here we review our current knowledge about anammox cell biology. The anammox cell is divided into three separate compartments by bilayer membranes. The anammox cell consists of (from outside to inside) the cell wall, paryphoplasm, riboplasm, and anammoxosome. Not much is known about the composition or function of both the anammox cell wall and the paryphoplasm compartment. The cell wall is proposed to be proteinaceous and to lack both peptidoglycan and an outer membrane typical of Gram-negative bacteria. The function of the paryphoplasm is unknown, but it contains the cell division ring. The riboplasm resembles the standard cytoplasmic compartment of other bacteria; it contains ribosomes and the nucleoid. The anammoxosome occupies most of the cell volume and is a so-called “prokaryotic organelle” analogous to the eukaryotic mitochondrion. This is the site where the anammox reaction takes place, coupled over the curved anammoxosome membrane, possibly giving rise to a proton motive force and subsequent ATP synthesis. With these unique properties, anammox bacteria are food for thought concerning the early evolution of the domains Bacteria, Archaea, and Eukarya. PMID:22933561

Searching for neuKREEP: An EMP study of Apollo 11 Group A basalts

NASA Technical Reports Server (NTRS)

Jerde, Eric A.; Taylor, Lawrence A.

1993-01-01

The Apollo 11 and 17 landing sites are characterized by the presence of high-Ti basalts (TiO2 greater than 6 percent). The Group A basalts of Apollo 11 have elevated K compositions (greater than 2000 ppm); and are enriched in incompatible trace elements relative to the other types of high-Ti basalt found in the region. These unique basalts also are the youngest of all high-Ti basalts, with an age of 3.56 +/- 0.02 Ga. Recent modelling of the Apollo 11 Group A basalts by Jerde et al. has demonstrated that this unique variety of high-Ti basalt may have formed through fractionation of a liquid with the composition of the Apollo 11 orange glass, coupled with assimilation of evolved material (dubbed neuKREEP and having similarities to lunar quartz monzodiorite). Assimilation of this material would impart its REE signature on the liquid, resulting in the elevated REE abundances observed. Minerals such as whitlockite which contain a large portion of the REE budget can be expected to reflect the REE characteristics of the assimilant. To this end, an examination of the whitlockite present in the Apollo 11 Group A basalts was undertaken to search for evidence of the neuKREEP material assimilated.

Macrophages phagocytose nonopsonized silica particles using a unique microtubule-dependent pathway

PubMed Central

Gilberti, Renée M.; Knecht, David A.

2015-01-01

Silica inhalation leads to the development of the chronic lung disease silicosis. Macrophages are killed by uptake of nonopsonized silica particles, and this is believed to play a critical role in the etiology of silicosis. However, the mechanism of nonopsonized-particle uptake is not well understood. We compared the molecular events associated with nonopsonized- and opsonized-particle phagocytosis. Both Rac and RhoA GTPases are activated upon nonopsonized-particle exposure, whereas opsonized particles activate either Rac or RhoA. All types of particles quickly generate a PI(3,4,5)P3 and F-actin response at the particle attachment site. After formation of a phagosome, the events related to endolysosome-to-phagosome fusion do not significantly differ between the pathways. Inhibitors of tyrosine kinases, actin polymerization, and the phosphatidylinositol cascade prevent opsonized- and nonopsonized-particle uptake similarly. Inhibition of silica particle uptake prevents silica-induced cell death. Microtubule depolymerization abolished uptake of complement-opsonized and nonopsonized particles but not Ab-opsonized particles. Of interest, regrowth of microtubules allowed uptake of new nonopsonized particles but not ones bound to cells in the absence of microtubules. Although complement-mediated uptake requires macrophages to be PMA-primed, untreated cells phagocytose nonopsonized silica and latex. Thus it appears that nonopsonized-particle uptake is accomplished by a pathway with unique characteristics. PMID:25428990

Science. [SITE 2001 Section].

ERIC Educational Resources Information Center

Roach, Linda E., Ed.

This document contains the following papers on science from the SITE (Society for Information Technology & Teacher Education) 2001 conference: (1) "Using a Computer Simulation before Dissection To Help Students Learn Anatomy" (Joseph Paul Akpan and Thomas Andre); (2) "EARTH2CLASS: A Unique Workshop/On-Line/Distance-Learning…

Chapter 19: Catalysis by Metal Carbides and Nitrides

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schaidle, Joshua A; Nash, Connor P; Yung, Matthew M

Early transition metal carbides and nitrides (ETMCNs), materials in which carbon or nitrogen occupies interstitial sites within a parent metal lattice, possess unique physical and chemical properties that motivate their use as catalysts. Specifically, these materials possess multiple types of catalytic sites, including metallic, acidic, and basic sites, and as such, exhibit reactivities that differ from their parent metals. Moreover, their surfaces are dynamic under reaction conditions. This chapter reviews recent (since 2010) experimental and computational investigations into the catalytic properties of ETMCN materials for applications including biomass conversion, syngas and CO2 upgrading, petroleum and natural gas refining, and electrocatalyticmore » energy conversion, energy storage, and chemicals production, and attempts to link catalyst performance to active site identity/surface structure in order to elucidate the present level of understanding of structure-function relationships for these materials. The chapter concludes with a perspective on leveraging the unique properties of these materials to design and develop improved catalysts through a dedicated, multidisciplinary effort.« less

Determinants of Visitor Pro-Environmental Intentions on Two Small Greek Islands: Is Ecotourism Possible at Coastal Protected Areas?

NASA Astrophysics Data System (ADS)

Kafyri, Andriani; Hovardas, Tasos; Poirazidis, Konstantinos

2012-07-01

A relatively under-researched question is whether there is a possibility of influencing environmentally aware tourists regarding ecotourism at destinations that continue to develop under a pattern of mass `seaside' tourism. Our objective was to assess the pro-environmental intentions of visitors at two small Greek islands, which are within a Natura 2000 site, specifically Paxoi and Antipaxoi. Intentions involved willingness to receive information about the protected area, willingness to accept pro-environmental limitations on recreational experience, and willingness-to-pay a conditional environmental conservation value added tax. In addition, we aimed to identify determinants of visitor pro-environmental intentions among visitor and visit characteristics, visitor satisfaction, and self-reported environmental knowledge, as well as anticipated outcomes of tourism development and suggestions for protected area management. We randomly collected 324 usable questionnaires during the summer season; 242 (74.69 %) by Greek visitors and 82 (25.31 %) by foreign visitors. Visitor satisfaction was quite high; however, visitors reported low levels of environmental knowledge. Our findings showed that the unique characteristics of the destination were not salient among visitors and that there is a lack of effective outreach campaigns, interpretation, and on-site environmental education programs. However, our study revealed high levels of visitor pro-environmental intentions that might support the promotion of ecotourism on the two islands. We provide recommendations based on determinants of visitor pro-environmental intentions, which might assist towards advancing visitor participation in environmental education projects, environmentally responsible behavior among visitors, and financial contribution to environmental conservation by visitors.

Determinants of visitor pro-environmental intentions on two small Greek islands: is ecotourism possible at coastal protected areas?

PubMed

Kafyri, Andriani; Hovardas, Tasos; Poirazidis, Konstantinos

2012-07-01

A relatively under-researched question is whether there is a possibility of influencing environmentally aware tourists regarding ecotourism at destinations that continue to develop under a pattern of mass 'seaside' tourism. Our objective was to assess the pro-environmental intentions of visitors at two small Greek islands, which are within a Natura 2000 site, specifically Paxoi and Antipaxoi. Intentions involved willingness to receive information about the protected area, willingness to accept pro-environmental limitations on recreational experience, and willingness-to-pay a conditional environmental conservation value added tax. In addition, we aimed to identify determinants of visitor pro-environmental intentions among visitor and visit characteristics, visitor satisfaction, and self-reported environmental knowledge, as well as anticipated outcomes of tourism development and suggestions for protected area management. We randomly collected 324 usable questionnaires during the summer season; 242 (74.69 %) by Greek visitors and 82 (25.31 %) by foreign visitors. Visitor satisfaction was quite high; however, visitors reported low levels of environmental knowledge. Our findings showed that the unique characteristics of the destination were not salient among visitors and that there is a lack of effective outreach campaigns, interpretation, and on-site environmental education programs. However, our study revealed high levels of visitor pro-environmental intentions that might support the promotion of ecotourism on the two islands. We provide recommendations based on determinants of visitor pro-environmental intentions, which might assist towards advancing visitor participation in environmental education projects, environmentally responsible behavior among visitors, and financial contribution to environmental conservation by visitors.

Human body as a set of biometric features identified by means of optoelectronics

NASA Astrophysics Data System (ADS)

Podbielska, Halina; Bauer, Joanna

2005-09-01

Human body posses many unique, singular features that are impossible to copy or forge. Nowadays, to establish and to ensure the public security requires specially designed devices and systems. Biometrics is a field of science and technology, exploiting human body characteristics for people recognition. It identifies the most characteristic and unique ones in order to design and construct systems capable to recognize people. In this paper some overview is given, presenting the achievements in biometrics. The verification and identification process is explained, along with the way of evaluation of biometric recognition systems. The most frequently human biometrics used in practice are shortly presented, including fingerprints, facial imaging (including thermal characteristic), hand geometry and iris patterns.

Site characteristics of red spruce witness tree locations in the uplands of West Virginia, USA

Treesearch

Melissa Thomas-Van Gundy; Michael Strager; James. Rentch

2012-01-01

Knowledge, both of the historical range of spruce-dominated forests and associated site conditions, is needed by land managers to help define restoration goals and potential sites for restoration. We used an existing digital database of witness trees listed in deeds from 1752 to 1899 to compare characteristics of red spruce (Picea rubens Sarg.) sites...

Vegetative characteristics of swift fox denning and foraging sites in southwestern South Dakota

Treesearch

Daniel W. Uresk; Kieth E. Severson; Jody Javersak

2003-01-01

Vegetative characteristics of swift fox (Vulpes velox) denning and foraging habitats were studied in southwestern South Dakota. We followed 14 radio-collared foxes over a two-year period and identified 17 den sites and 82 foraging sites. Height-density of vegetation (visual obstruction reading, VOR) was determined on each den and foraging site and on...

Measuring corporate culture to ensure mission fulfillment.

PubMed

Hulsebus-Fong, C

1988-11-01

Ancilla Systems, Inc., Elk Grove Village, Il, developed a mission-based performance evaluation program to provide tangible evidence of mission fulfillment and ensure the provision of high-quality healthcare. the program--Characteristics of Service--translates the language of healthcare action and evaluates corporate culture to ensure that it fulfills the expectations of its sponsor. The nine Characteristics of Service are: Respect for the dignity of all persons. Orientation toward the family unit. Quality and personalized services. Local health systems with a spectrum of services responsive to the unique needs of the community. Formal and informal partnerships with physicians. Active participation and collaboration with related community service agencies and other healthcare providers. Faithfulness to Catholic identity through close relationships with Church and religious institute resources. Effective political advocacy through education. Research and development of innovative approaches to healthcare. In establishing the behavior standards that would exemplify the characteristics, program developers used terms that correspond to specific, observable, measurable performance. All healthcare facilities are evaluated on how well they meet the behavior standards. The evaluation process includes data collection, analysis, and a final report. Data collection begins with a review of regular hospital-conducted surveys, which provide quantifiable information to measure performance against key expected behaviors. Additional data are derived from medical staff development plans and the monthly quality assurance audit. On-site surveys fill information gaps that remain after all written reports are collected.

Analysis of the Seismic Events Apparently Associated with the 3 September 2017 DPRK Declared Nuclear Explosion

NASA Astrophysics Data System (ADS)

Walter, W. R.; Dodge, D. A.; Ichinose, G.; Myers, S. C.; Ford, S. R.; Pitarka, A.; Pyle, M. L.; Pasyanos, M.; Matzel, E.; Rodgers, A. J.; Mellors, R. J.; Hauk, T. F.; Kroll, K.

2017-12-01

On September 3, 2017, an mb 6.3 seismic event was reported by the USGS in the vicinity of the DPRK nuclear test site at Punggye-ri. Shortly afterwards DPRK declared it had conducted a nuclear explosion. The seismic signals indicate this event is roughly an order of magnitude larger than the largest of the previous five DPRK declared nuclear tests. In addition to its size, this explosion was different from previous DPRK tests in being associated with a number of additional seismic events. Approximately eight and a half minutes after the explosion a seismic event reported as ML 4.0 by the USGS occurred. Regional waveform modeling indicated this event had a collapse mechanism (e.g. Ichinose et al., 2017, written communication). On September 23 and again on October 12, 2017, seismic events were reported near the DPRK test site by the USGS and the CTBTO (on 9/23/17 two events: USGS ML 3.6 and USGS ML 2.6; and on 10/12/17 one event: USGS mb(Lg) 2.9). Aftershocks following underground nuclear testing are expected, though at much lower magnitudes and rates than for comparably sized earthquakes. This difference in aftershock production has been proposed by Ford and Walter (2010), and others as a potential source-type discriminant. Seismic signals from the collapse of cavities formed by underground nuclear testing have also been previously observed. For example, the mb 5.7 nuclear test ATRISCO in Nevada in 1982 was followed twenty minutes later by a collapse with an mb of 4.0. Here we examine the seismic characteristics of nuclear tests, post-test collapses and post-test aftershocks from both the former Nevada test site and the DPRK test site to better understand the differences between these different source-type signals. In particular we look at discriminants such as P/S ratios, to see if there are unique characteristics to post-test collapses and aftershocks. Finally, we apply correlation methods to continuous data at regional stations to look for additional seismic signals that might have an apparent association with the DPRK nuclear testing, post-testing collapses and post-test induced seismicity.

The multiple nucleotide-divalent cation binding modes of Saccharomyces cerevisiae CK2α indicate a possible co-substrate hydrolysis product (ADP/GDP) release pathway.

PubMed

Liu, Huihui; Wang, Hong; Teng, Maikun; Li, Xu

2014-02-01

CK2 is a ubiquitous and conserved protein kinase in eukaryotic organisms and is important in many biological processes. It is unique in maintaining constitutive activity and in using both ATP and GTP as phosphor donors. In this study, crystal structures of recombinant Saccharomyces cerevisiae CK2α (scCK2α) complexed with GMPPNP, ATP and AMPPN with either Mg2+ or Mn2+ as the coordinated divalent cation are presented. The overall structure of scCK2α shows high similarity to its homologous proteins by consisting of two domains with the co-substrate lying in the cleft between them. However, three characteristic features distinguish scCK2α from its homologues. Firstly, the Lys45-Glu53 and Arg48-Glu53 interactions in scCK2α lead Lys50 to adopt a unique conformation that is able to stabilize the γ-phosphate of the co-substrate, which makes the existence of the `essential divalent cation' not so essential. The multiple nucleotide-divalent cation binding modes of the active site of scCK2α are apparently different from the two-divalent-cation-occupied active site of Zea mays CK2α and human CK2α. Secondly, conformational change of Glu53 in scCK2α-AMPPN breaks its interaction with Lys45 and Arg48; as a result, the co-substrate binding pocket becomes more open. This may suggest a clue to a possible ADP/GDP-release pathway, because the NE1 atom of the Trp in the `DWG motif' of CK2α forms a hydrogen bond to the O atom of Leu212, which seems to make ADP release by means of the `DFG-in flip to DFG-out' model found in most eukaryotic protein kinases impossible. Coincidentally, two sulfate ions which may mimic two phosphate groups were captured by Arg161 and Lys197 around the pocket. Mutagenesis and biochemical experiments on R161A and K197A mutants support the above proposal. Finally, scCK2α is unique in containing an insertion region whose function had not been identified in previous research. It is found that the insertion region contributes to maintaining the constitutively active conformation of the scCK2α catalytic site, but does not participate in interaction with the regulatory subunits.

Nitrogen-Doped Carbon Nanotube/Graphite Felts as Advanced Electrode Materials for Vanadium Redox Flow Batteries.

PubMed

Wang, Shuangyin; Zhao, Xinsheng; Cochell, Thomas; Manthiram, Arumugam

2012-08-16

Nitrogen-doped carbon nanotubes have been grown, for the first time, on graphite felt (N-CNT/GF) by a chemical vapor deposition approach and examined as an advanced electrode for vanadium redox flow batteries (VRFBs). The unique porous structure and nitrogen doping of N-CNT/GF with increased surface area enhances the battery performance significantly. The enriched porous structure of N-CNTs on graphite felt could potentially facilitate the diffusion of electrolyte, while the N-doping could significantly contribute to the enhanced electrode performance. Specifically, the N-doping (i) modifies the electronic properties of CNT and thereby alters the chemisorption characteristics of the vanadium ions, (ii) generates defect sites that are electrochemically more active, (iii) increases the oxygen species on CNT surface, which is a key factor influencing the VRFB performance, and (iv) makes the N-CNT electrochemically more accessible than the CNT.

Evaluation of the usefulness of various simulation technology options for TERPS enhancement

NASA Technical Reports Server (NTRS)

Phatak, A. V.; Sorensen, J. A.

1986-01-01

Current approved terminal instrument procedures (TERPS) do not permit the full exploitation of the helicopter's unique flying characteristics. Enhanced TERPS need to be developed for a host of non-standard landing sites and navigation aids. Precision navigation systems such as microwave landing systems (MLS) and the Global Positioning System (GPS) open the possibility of curved paths, steep glide slopes, and decelerating helicopter approaches. This study evaluated the feasibility, benefits, and liabilities of using helicopter cockpit simulators in place of flight testing to develop enhanced TERPS criteria for non-standard flight profiles and navigation equipment. Near-term (2 to 5 year) requirements for conducting simulator studies to verify that they produce suitable data comparable to that obtained from previous flight tests are discussed. The long-term (5 to 10 year) research and development requirements to provide necessary modeling for continued simulator-based testing to develop enhanced TERPS criteria are also outlined.

Implementation of two new resource management information systems in Australia

NASA Astrophysics Data System (ADS)

Kessell, Stephen R.; Good, Roger B.; Hopkins, Angas J. M.

1984-05-01

This paper describes the development and implementation of PREPLAN, A Pristine Environment Planning Language and Simulator, for two conservation areas in Australia, Kosciusko National Park (New South Wales) and Tutanning Nature Reserve (Western Australia). PREPLAN was derived from the North American gradient modeling systems and the Forest Planning Language and Simulator (FORPLAN), but includes unique characteristics not previously available. PREPLAN includes an integrated resource management data base, modules for predicting site-specific vegetation, fuels, animals, fire behavior, and fire effects, and an English language instruction set. PREPLAN was developed specifically to provide available information and understanding of ecosystems to managers in a readily accessible and usable form, and to provide the motivation to conduct additional required research projects. An evaluation of the system's advantages and limitations is presented, and the way the utilization of such systems is improving natural area decision making throughout Australia is discussed.

Liver-resident NK cells and their potential functions.

PubMed

Peng, Hui; Sun, Rui

2017-09-18

Natural killer (NK) cells represent a heterogeneous population of innate lymphocytes with phenotypically and functionally distinct subsets. In particular, recent studies have identified a unique subset of NK cells residing within the liver that are maintained as tissue-resident cells, confer antigen-specific memory responses and exhibit different phenotypical and developmental characteristics compared with conventional NK (cNK) cells. These findings have encouraged researchers to uncover tissue-resident NK cells at other sites, and detailed analyses have revealed that these tissue-resident NK cells share many similarities with liver-resident NK cells and tissue-resident memory T cells. Here, we present a brief historical perspective on the discovery of liver-resident NK cells and discuss their relationship to cNK cells and other emerging NK cell subsets and their potential functions.Cellular &Molecular Immunology advance online publication, 18 September 2017; doi:10.1038/cmi.2017.72.

Patient-centered medical home model: do school-based health centers fit the model?

PubMed

Larson, Satu A; Chapman, Susan A

2013-01-01

School-based health centers (SBHCs) are an important component of health care reform. The SBHC model of care offers accessible, continuous, comprehensive, family-centered, coordinated, and compassionate care to infants, children, and adolescents. These same elements comprise the patient-centered medical home (PCMH) model of care being promoted by the Affordable Care Act with the hope of lowering health care costs by rewarding clinicians for primary care services. PCMH survey tools have been developed to help payers determine whether a clinician/site serves as a PCMH. Our concern is that current survey tools will be unable to capture how a SBHC may provide a medical home and therefore be denied needed funding. This article describes how SBHCs might meet the requirements of one PCMH tool. SBHC stakeholders need to advocate for the creation or modification of existing survey tools that allow the unique characteristics of SBHCs to qualify as PCMHs.

Snake velvet black: Hierarchical micro- and nanostructure enhances dark colouration in Bitis rhinoceros

NASA Astrophysics Data System (ADS)

Spinner, Marlene; Kovalev, Alexander; Gorb, Stanislav N.; Westhoff, Guido

2013-05-01

The West African Gaboon viper (Bitis rhinoceros) is a master of camouflage due to its colouration pattern. Its skin is geometrically patterned and features black spots that purport an exceptional spatial depth due to their velvety surface texture. Our study shades light on micromorphology, optical characteristics and principles behind such a velvet black appearance. We revealed a unique hierarchical pattern of leaf-like microstructures striated with nanoridges on the snake scales that coincides with the distribution of black colouration. Velvet black sites demonstrate four times lower reflectance and higher absorbance than other scales in the UV - near IR spectral range. The combination of surface structures impeding reflectance and absorbing dark pigments, deposited in the skin material, provides reflecting less than 11% of the light reflected by a polytetrafluoroethylene diffuse reflectance standard in any direction. A view-angle independent black structural colour in snakes is reported here for the first time.

Graphene and graphene nanocomposites for the removal of aromatic organic compounds from the water: systematic review

NASA Astrophysics Data System (ADS)

Monsores Paixão, Monique; Tadeu Gomes Vianna, Marco; Marques, Marcia

2018-01-01

Aromatic organic pollutants are highly toxic to the human and environmental health and are considered as priority pollutants by regulatory agencies. Managing contaminated sites with organic pollutants is one of the major environmental challenges today. Of all technologies that have been proposed to remove contaminants, adsorption is recognized worldwide as an attractive option due to its versatility, wide applicability and economic viability. Recent studies report the use of graphene (GN), a recently carbon nanomaterial, and its derivatives in sorption processes for the removal of aromatic organic compounds. The present review has shown that GN structures are a promising alternative to traditional adsorbent materials, with excellent results in the removal of organic compounds from water, due to their unique structural characteristics and great adsorption capacity for organic compounds. Although, there is still a long way to go until that practical applications can be implemented.

Endothelin induces two types of contractions of rat uterus: phasic contractions by way of voltage-dependent calcium channels and developing contractions through a second type of calcium channels

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kozuka, M.; Ito, T.; Hirose, S.

1989-02-28

Effects of endothelin on nonvascular smooth muscle have been examined using rat uterine horns and two modes of endothelin action have been revealed. Endothelin (0.3 nM) caused rhythmic contractions of isolated uterus in the presence of extracellular calcium. The rhythmic contractions were completely inhibited by calcium channel antagonists. These characteristics of endothelin-induced contractions were very similar to those induced by oxytocin. Binding assays using /sup 125/I-endothelin showed that endothelin and the calcium channel blockers did not compete for the binding sites. However, endothelin was unique in that it caused, in addition to rhythmic contractions, a slowly developing monophasic contraction thatmore » was insensitive to calcium channel blockers. This developing contraction became dominant at higher concentrations of endothelin and was also calcium dependent.« less

Field test analysis of concentrator photovoltaic system focusing on average photon energy and temperature

NASA Astrophysics Data System (ADS)

Husna, Husyira Al; Ota, Yasuyuki; Minemoto, Takashi; Nishioka, Kensuke

2015-08-01

The concentrator photovoltaic (CPV) system is unique and different from the common flat-plate PV system. It uses a multi-junction solar cell and a Fresnel lens to concentrate direct solar radiation onto the cell while tracking the sun throughout the day. The cell efficiency could reach over 40% under high concentration ratio. In this study, we analyzed a one year set of environmental condition data of the University of Miyazaki, Japan, where the CPV system was installed. Performance ratio (PR) was discussed to describe the system’s performance. Meanwhile, the average photon energy (APE) was used to describe the spectrum distribution at the site where the CPV system was installed. A circuit simulator network was used to simulate the CPV system electrical characteristics under various environmental conditions. As for the result, we found that the PR of the CPV systems depends on the APE level rather than the cell temperature.

Street as Public Space - Measuring Street Life of Kuala Lumpur

NASA Astrophysics Data System (ADS)

Sulaiman, Normah; Ayu Abdullah, Yusfida; Hamdan, Hazlina

2017-10-01

Kuala Lumpur has envisioning in becoming World Class City by the year 2020. Essential elements of form and function of the urban environment are streets. Streets showcase the community and connect people. It’s one of the most comfortable social environment that provides aesthetical and interaction pleasure for everyone. Classified as main shopping streets in the local Kuala Lumpur urban design guidelines, Jalan Masjid India (JMI) has its uniqueness of shopping experience and social interaction. This conceptual paper will study the physical and cultural characteristics of the street that will generate the street character by mapping its original characters. The findings will focus on strengthening the methodology applied to promote improvements in evaluating it as a great public space. Results will also contribute to understanding the overall site context, the street connectivity, and urban dynamics. This paper is part of a larger study that addresses on transforming the sociability of public space.

High Voltage Power Transmission for Wind Energy

NASA Astrophysics Data System (ADS)

Kim, Young il

The high wind speeds and wide available area at sea have recently increased the interests on offshore wind farms in the U.S.A. As offshore wind farms become larger and are placed further from the shore, the power transmission to the onshore grid becomes a key feature. Power transmission of the offshore wind farm, in which good wind conditions and a larger installation area than an onshore site are available, requires the use of submarine cable systems. Therefore, an underground power cable system requires unique design and installation challenges not found in the overhead power cable environment. This paper presents analysis about the benefit and drawbacks of three different transmission solutions: HVAC, LCC/VSC HVDC in the grid connecting offshore wind farms and also analyzed the electrical characteristics of underground cables. In particular, loss of HV (High Voltage) subsea power of the transmission cables was evaluated by the Brakelmann's theory, taking into account the distributions of current and temperature.

A LANDSAT study of ephemeral and perennial rangeland vegetation and soils

NASA Technical Reports Server (NTRS)

Bentley, R. G., Jr. (Principal Investigator); Salmon-Drexler, B. C.; Bonner, W. J.; Vincent, R. K.

1976-01-01

The author has identified the following significant results. Several methods of computer processing were applied to LANDSAT data for mapping vegetation characteristics of perennial rangeland in Montana and ephemeral rangeland in Arizona. The choice of optimal processing technique was dependent on prescribed mapping and site condition. Single channel level slicing and ratioing of channels were used for simple enhancement. Predictive models for mapping percent vegetation cover based on data from field spectra and LANDSAT data were generated by multiple linear regression of six unique LANDSAT spectral ratios. Ratio gating logic and maximum likelihood classification were applied successfully to recognize plant communities in Montana. Maximum likelihood classification did little to improve recognition of terrain features when compared to a single channel density slice in sparsely vegetated Arizona. LANDSAT was found to be more sensitive to differences between plant communities based on percentages of vigorous vegetation than to actual physical or spectral differences among plant species.

Involvement of opsins in mammalian sperm thermotaxis

PubMed Central

Pérez-Cerezales, Serafín; Boryshpolets, Sergii; Afanzar, Oshri; Brandis, Alexander; Nevo, Reinat; Kiss, Vladimir; Eisenbach, Michael

2015-01-01

A unique characteristic of mammalian sperm thermotaxis is extreme temperature sensitivity, manifested by the capacity of spermatozoa to respond to temperature changes of <0.0006 °C as they swim their body-length distance. The identity of the sensing system that confers this exceptional sensitivity on spermatozoa is not known. Here we show that the temperature-sensing system of mammalian spermatozoa involves opsins, known to be G-protein-coupled receptors that act as photosensors in vision. We demonstrate by molecular, immunological, and functional approaches that opsins are present in human and mouse spermatozoa at specific sites, which depend on the species and the opsin type, and that they are involved in sperm thermotaxis via two signalling pathways—the phospholipase C and the cyclic-nucleotide pathways. Our results suggest that, depending on the context and the tissue, mammalian opsins act not only as photosensors but also as thermosensors. PMID:26537127

Snake velvet black: hierarchical micro- and nanostructure enhances dark colouration in Bitis rhinoceros.

PubMed

Spinner, Marlene; Kovalev, Alexander; Gorb, Stanislav N; Westhoff, Guido

2013-01-01

The West African Gaboon viper (Bitis rhinoceros) is a master of camouflage due to its colouration pattern. Its skin is geometrically patterned and features black spots that purport an exceptional spatial depth due to their velvety surface texture. Our study shades light on micromorphology, optical characteristics and principles behind such a velvet black appearance. We revealed a unique hierarchical pattern of leaf-like microstructures striated with nanoridges on the snake scales that coincides with the distribution of black colouration. Velvet black sites demonstrate four times lower reflectance and higher absorbance than other scales in the UV-near IR spectral range. The combination of surface structures impeding reflectance and absorbing dark pigments, deposited in the skin material, provides reflecting less than 11% of the light reflected by a polytetrafluoroethylene diffuse reflectance standard in any direction. A view-angle independent black structural colour in snakes is reported here for the first time.

3' Homologous Free Ends are Required for Stable Joint Molecule Formation by the RecA and Single-Stranded Binding Proteins of Escherichia coli

NASA Astrophysics Data System (ADS)

Konforti, Boyana B.; Davis, Ronald W.

1987-02-01

The RecA protein of Escherichia coli is important for genetic recombination in vivo and can promote synapsis and strand exchange in vitro. The DNA pairing and strand exchange reactions have been well characterized in reactions with circular single strands and linear duplexes, but little is known about these two processes using substrates more characteristic of those likely to exist in the cell. Single-stranded linear DNAs were prepared by separating strands of duplex molecules or by cleaving single-stranded circles at a unique restriction site created by annealing a short defined oligonucleotide to the circle. Analysis by gel electrophoresis and electron microscopy revealed that, in the presence of RecA and single-stranded binding proteins, a free 3' homologous end is essential for stable joint molecule formation between linear single-stranded and circular duplex DNA.

INVESTIGATING UNCERTAINTY AND SENSITIVITY IN INTEGRATED, MULTIMEDIA ENVIRONMENTAL MODELS: TOOLS FOR FRAMES-3MRA

EPA Science Inventory

Elucidating uncertainty and sensitivity structures in environmental models can be a difficult task, even for low-order, single-medium constructs driven by a unique set of site-specific data. Quantitative assessment of integrated, multimedia models that simulate hundreds of sites...

30 CFR 46.11 - Site-specific hazard awareness training.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Section 46.11 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR EDUCATION AND... workers; (4) Customers, including commercial over-the-road truck drivers; (5) Construction workers or... procedures. The training must address site-specific health and safety risks, such as unique geologic or...

10 CFR 100.10 - Factors to be considered when evaluating sites.

Code of Federal Regulations, 2013 CFR

2013-01-01

... reactor incorporates unique or unusual features having a significant bearing on the probability or consequences of accidental release of radioactive materials; (4) The safety features that are to be engineered... radioactive fission products. In addition, the site location and the engineered features included as...

10 CFR 100.10 - Factors to be considered when evaluating sites.

Code of Federal Regulations, 2012 CFR

2012-01-01

... reactor incorporates unique or unusual features having a significant bearing on the probability or consequences of accidental release of radioactive materials; (4) The safety features that are to be engineered... radioactive fission products. In addition, the site location and the engineered features included as...

10 CFR 100.10 - Factors to be considered when evaluating sites.

Code of Federal Regulations, 2014 CFR

2014-01-01

... reactor incorporates unique or unusual features having a significant bearing on the probability or consequences of accidental release of radioactive materials; (4) The safety features that are to be engineered... radioactive fission products. In addition, the site location and the engineered features included as...

Reflections on Museums as Effective Field Sites for Teacher Candidates

ERIC Educational Resources Information Center

Clark, Megan; Ensminger, David; Incandela, Colleen; Moisan, Heidi

2016-01-01

A unique partnership among six museums and Loyola University Chicago's "Teaching Learning and Leading with Schools and Communities" teacher preparation program provided cross-disciplinary field sites for understanding and witnessing developmental and learning theories. Pre-service teacher candidates were able to identify constructs and…

Digital Biological Converter

DTIC Science & Technology

2013-06-28

of cuts that each fragment should be cut into so the fragments are no greater than a specific length threshold. Additionally, vector sequences and...restriction sites are attached to each fragment while ensuring the restriction sites are unique to each sequence. The vector sequences serve as hooks...for assembly into vector for cloning purposes, and also as primer binding domains for PCR ampl ification. The restriction sites are added to

Spatial analysis of agri-environmental policy uptake and expenditure in Scotland.

PubMed

Yang, Anastasia L; Rounsevell, Mark D A; Wilson, Ronald M; Haggett, Claire

2014-01-15

Agri-environment is one of the most widely supported rural development policy measures in Scotland in terms of number of participants and expenditure. It comprises 69 management options and sub-options that are delivered primarily through the competitive 'Rural Priorities scheme'. Understanding the spatial determinants of uptake and expenditure would assist policy-makers in guiding future policy targeting efforts for the rural environment. This study is unique in examining the spatial dependency and determinants of Scotland's agri-environmental measures and categorised options uptake and payments at the parish level. Spatial econometrics is applied to test the influence of 40 explanatory variables on farming characteristics, land capability, designated sites, accessibility and population. Results identified spatial dependency for each of the dependent variables, which supported the use of spatially-explicit models. The goodness of fit of the spatial models was better than for the aspatial regression models. There was also notable improvement in the models for participation compared with the models for expenditure. Furthermore a range of expected explanatory variables were found to be significant and varied according to the dependent variable used. The majority of models for both payment and uptake showed a significant positive relationship with SSSI (Sites of Special Scientific Interest), which are designated sites prioritised in Scottish policy. These results indicate that environmental targeting efforts by the government for AEP uptake in designated sites can be effective. However habitats outside of SSSI, termed here the 'wider countryside' may not be sufficiently competitive to receive funding in the current policy system. Copyright © 2013 Elsevier Ltd. All rights reserved.

Contemporaneous trace and body fossils from a late Pleistocene Lakebed in Victoria, Australia, allow assessment of bias in the fossil record.

PubMed

Camens, Aaron Bruce; Carey, Stephen Paul

2013-01-01

The co-occurrence of vertebrate trace and body fossils within a single geological formation is rare and the probability of these parallel records being contemporaneous (i.e. on or near the same bedding plane) is extremely low. We report here a late Pleistocene locality from the Victorian Volcanic Plains in south-eastern Australia in which demonstrably contemporaneous, but independently accumulated vertebrate trace and body fossils occur. Bite marks from a variety of taxa are also present on the bones. This site provides a unique opportunity to examine the biases of these divergent fossil records (skeletal, footprints and bite marks) that sampled a single fauna. The skeletal record produced the most complete fauna, with the footprint record indicating a markedly different faunal composition with less diversity and the feeding traces suggesting the presence, amongst others, of a predator not represented by either the skeletal or footprint records. We found that the large extinct marsupial predator Thylacoleo was the only taxon apparently represented by all three records, suggesting that the behavioral characteristics of large carnivores may increase the likelihood of their presence being detected within a fossil fauna. In contrast, Diprotodon (the largest-ever marsupial) was represented only by trace fossils at this site and was absent from the site's skeletal record, despite its being a common and easily detected presence in late Pleistocene skeletal fossil faunas elsewhere in Australia. Small mammals absent from the footprint record for the site were represented by skeletal fossils and bite marks on bones.

Contemporaneous Trace and Body Fossils from a Late Pleistocene Lakebed in Victoria, Australia, Allow Assessment of Bias in the Fossil Record

PubMed Central

Camens, Aaron Bruce; Carey, Stephen Paul

2013-01-01

The co-occurrence of vertebrate trace and body fossils within a single geological formation is rare and the probability of these parallel records being contemporaneous (i.e. on or near the same bedding plane) is extremely low. We report here a late Pleistocene locality from the Victorian Volcanic Plains in south-eastern Australia in which demonstrably contemporaneous, but independently accumulated vertebrate trace and body fossils occur. Bite marks from a variety of taxa are also present on the bones. This site provides a unique opportunity to examine the biases of these divergent fossil records (skeletal, footprints and bite marks) that sampled a single fauna. The skeletal record produced the most complete fauna, with the footprint record indicating a markedly different faunal composition with less diversity and the feeding traces suggesting the presence, amongst others, of a predator not represented by either the skeletal or footprint records. We found that the large extinct marsupial predator Thylacoleo was the only taxon apparently represented by all three records, suggesting that the behavioral characteristics of large carnivores may increase the likelihood of their presence being detected within a fossil fauna. In contrast, Diprotodon (the largest-ever marsupial) was represented only by trace fossils at this site and was absent from the site's skeletal record, despite its being a common and easily detected presence in late Pleistocene skeletal fossil faunas elsewhere in Australia. Small mammals absent from the footprint record for the site were represented by skeletal fossils and bite marks on bones. PMID:23301008

Building a Global Network of Hydro-climatology Sites in Cloud-affected Tropical Montane Forests

NASA Astrophysics Data System (ADS)

Moore, G. W.; Asbjornsen, H.; Bruijnzeel, S., Sr.; Berry, Z. C.; Giambelluca, T. W.; Martin, P.; Mulligan, M.

2015-12-01

Tropical montane forests are characteristically wet environments with low evapotranspiration and sometimes significant contributions from fog interception. They are often located at headwater catchments critical for water supplies, but ecohydroclimate data in these regions are sparse. Such evidence may be crucial for assessing climate alterations in these sensitive ecosystems. As part of a global effort led by the Tropical Montane Cloud Forest Research Coordination Network (Cloudnet - http://cloudnet.agsci.colostate.edu), we aim to extend the network of tropical montane forest sites and establish robust protocols for measuring key ecohydroclimatic parameters, including fog interception, windblown rain, throughfall, leaf wetness, and micrometeorological conditions. Specific recommendations for standardized protocols include (1) rain and fog collectors uniquely designed to separately quantify fog interception from direct rain inputs, even in windy conditions, (2) trough-style throughfall gages that collect 40 times the area of a typical tipping bucket gage with added features to reduce splash-out, (3) clusters of leaf wetness sensors to differentiate frequency and duration of wetness caused by rain and fog on windward and leeward exposures, and (4) basic micrometeorological sensors for solar radiation, temperature, humidity, and wind. At sites where resources allow for additional measurements, we developed protocols for quantifying soil moisture, soil saturation, and plant water uptake from both roots and leaves (i.e. foliar absorption), since these are also important drivers in these systems. Participating sites will be invited to contribute to a global meta-analysis that will provide new insights into the ecohydrology of cloud-affected tropical montane forests.

Unusual Characteristics of the DNA Binding Domain of Epigenetic Regulatory Protein MeCP2 Determine Its Binding Specificity

PubMed Central

2015-01-01

The protein MeCP2 mediates epigenetic regulation by binding methyl-CpG (mCpG) sites on chromatin. MeCP2 consists of six domains of which one, the methyl binding domain (MBD), binds mCpG sites in duplex DNA. We show that solution conditions with physiological or greater salt concentrations or the presence of nonspecific competitor DNA is necessary for the MBD to discriminate mCpG from CpG with high specificity. The specificity for mCpG over CpG is >100-fold under these solution conditions. In contrast, the MBD does not discriminate hydroxymethyl-CpG from CpG. The MBD is unusual among site-specific DNA binding proteins in that (i) specificity is not conferred by the enhanced affinity for the specific site but rather by suppression of its affinity for generic DNA, (ii) its specific binding to mCpG is highly electrostatic, and (iii) it takes up as well as displaces monovalent cations upon DNA binding. The MBD displays an unusually high affinity for single-stranded DNA independent of modification or sequence. In addition, the MBD forms a discrete dimer on DNA via a noncooperative binding pathway. Because the affinity of the second monomer is 1 order of magnitude greater than that of nonspecific binding, the MBD dimer is a unique molecular complex. The significance of these results in the context of neuronal function and development and MeCP2-related developmental disorders such as Rett syndrome is discussed. PMID:24828757

49 CFR 325.53 - Site characteristics; stationary test.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Section 325.53 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Stationary Test § 325.53 Site characteristics; stationary test. (a)(1) The motor vehicle to be tested shall be parked on the test site. A...

49 CFR 325.53 - Site characteristics; stationary test.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Section 325.53 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Stationary Test § 325.53 Site characteristics; stationary test. (a)(1) The motor vehicle to be tested shall be parked on the test site. A...

49 CFR 325.53 - Site characteristics; stationary test.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Section 325.53 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Stationary Test § 325.53 Site characteristics; stationary test. (a)(1) The motor vehicle to be tested shall be parked on the test site. A...

49 CFR 325.53 - Site characteristics; stationary test.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Section 325.53 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Stationary Test § 325.53 Site characteristics; stationary test. (a)(1) The motor vehicle to be tested shall be parked on the test site. A...

49 CFR 325.53 - Site characteristics; stationary test.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Section 325.53 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR... MOTOR CARRIER NOISE EMISSION STANDARDS Measurement of Noise Emissions; Stationary Test § 325.53 Site characteristics; stationary test. (a)(1) The motor vehicle to be tested shall be parked on the test site. A...

Construction of a polyhedron decorated MOF with a unique network through the combination of two classic secondary building units.

PubMed

Ren, Guo-Jian; Chang, Ze; Xu, Jian; Hu, Zhenpeng; Liu, Yan-Qing; Xu, Yue-Ling; Bu, Xian-He

2016-02-04

A novel decorated metal-organic polyhedron (MOP) based metal-organic framework with a unique 4,9-connected network is successfully constructed, which displays a relatively strong interaction toward H2 and CO2 probably due to the existence of open metal sites in the secondary building units.

Solute-specific patterns and drivers of urban stream chemistry revealed by long-term monitoring in Baltimore, Maryland

NASA Astrophysics Data System (ADS)

Reisinger, A. J.; Woytowitz, E.; Majcher, E.; Rosi, E. J.; Groffman, P.

2017-12-01

Urban streams receive a myriad of chemical inputs from the surrounding landscape due to altered lithology (asphalt, concrete), leaky sewage infrastructure, and other human activities (road salt, fertilizer, industrial wastes, wastewater effluent), potentially leading to multiple chemical stressors occurring simultaneously. To evaluate potential drivers of water chemistry change, we used approximately 20 years of weekly water chemistry monitoring data from streams in the Baltimore Ecosystem Study (BES) to quantify trends of annual loads and flow-weighted concentrations for multiple solutes of interest, including nitrate (NO3-), phosphate (PO43-), total nitrogen (TN), total phosphorus (TP), chloride (Cl-), and sulfate (SO42-) and subsequently examined various gray and green infrastructure characteristics at the watershed scale. For example, we quantified annual volume and duration of reported sanitary sewer overflows (SSO) and cumulative storage volume and area of various best management practices (BMPs). Site- and solute-specific trends differed, but across our monitoring network we found evidence for decreasing annual export for multiple solutes. Additionally, we found that changes in gray- and green-infrastructure characteristics were related to changes in water quality at our most downstream (most urban) monitoring site. For example, annual NO3- loads increased with longer cumulative SSO duration, whereas annual PO43- and TP loads decreased with a cumulative BMP area in the watershed. Further, we used same long-term water chemistry data and multivariate analyses to investigate whether urban streams have unique water chemistry fingerprints representing the multiple chemical stressors at a given site, which could provide insight into sources and impacts of water-quality impairment. These analyses and results illustrate the major role gray and green infrastructure play in influencing water quality in urban environments, and illustrate that focusing on a variety of chemical stressors is necessary to gain a broader understanding of the issues affecting urban water quality.

Fingerprints and cardiovascular risk. The San Valentino fingerprint vascular screening project (SanVal/FP).

PubMed

Belcaro, G; Cesarone, M R; Ledda, A; Cornelli, U; Dugall, M; Di Renzo, A; Hosoi, M; Stuard, S; Vinciguerra, G; Pellegrini, L; Gizzi, G

2008-10-01

Fingerprints (FP), characteristic of humans, are impressions due to skin marks (ridges) on fingertips. Ridges are present on fingers/hands forming curved lines of different sizes/patterns. The point where a line stops or splits is defined typica' (their number/amount constitute identification patterns). FP are permanent and unique. This study compared FP patterns with cardiovascular risk factors: 7 main types of FP were used: 1. Arch: lines form waves from one site to the other side. 2. Tentarch: like arches but with a rising stick in the middle. 3. Loop: lines coming from one site returning in the middle to the same site. 4. Double loop: like loops but with two loops inside: one standing, one hanging. 5. Pocked loop: like the loop but with a small circle in the turning point. 6. Whorl: lines make circles. 7. Mixed figure: composed of different figures. There are two kinds of real typica: A. Ending line; B. Splitting lines (bifurcations). Several combinations may result. Ultrasound evaluation of carotid/femoral arteries in asymptomatic subjects. Arteries were evaluated with high-resolution ultrasound at the bifurcations. Four classes were defined: 1: normal intima-media (IMT) complex; 2: IMT thickening; 3: non-stenosing plaques (<50% stenosis); 4: stenosing plaque (>50%). Subjects in classes 1, 2, 3 were included into the analysis made comparing FP patterns and ultrasound. For each FP pattern: A. the main proportion of subjects with cardiovacular risk factors (91%) had arches (41.2%) and loops (either single, 38.2% or double 11.7% for a total of 49.9%). B. The remaining classes were statistically less important. C. The number of ridges per square mm was comparable in all pattern classes. D. The analysis of typica and other ridges characteristics requires a more elaborated system. Future research must define simple, low cost screening methods for preselection of subjects at higher cardiovascular risk or for exclusion of low risk subjects. The evaluation of fingerprint pattern may be useful to define risk groups.

Practical relevance of pattern uniqueness in forensic science.

PubMed

Jayaprakash, Paul T

2013-09-10

Uniqueness being unprovable, it has recently been argued that individualization in forensic science is irrelevant and, probability, as applied for DNA profiles, should be applied for all identifications. Critiques against uniqueness have omitted physical matching, a realistic and tangible individualization that supports uniqueness. Describing case examples illustrating pattern matches including physical matching, it is indicated that individualizations are practically relevant for forensic science as they establish facts on a definitive basis providing firm leads benefitting criminal investigation. As a tenet of forensic identification, uniqueness forms a fundamental paradigm relevant for individualization. Evidence on the indeterministic and stochastic causal pathways of characteristics in patterns available in the related fields of science sufficiently supports the proposition of uniqueness. Characteristics involved in physical matching and matching achieved in patterned evidence existing in the state of nature are not events amenable for counting; instead these are ensemble of visible units occupying the entire pattern area stretching the probability of re-occurrence of a verisimilitude pattern into infinity offering epistemic support to uniqueness. Observational methods are as respectable as instrumental or statistical methods since they are capable of generating results that are tangible and obviously valid as in physical matching. Applying the probabilistic interpretation used for DNA profiles to the other patterns would be unbefitting since these two are disparate, the causal pathways of the events, the loci, in the manipulated DNA profiles being determinable. While uniqueness enables individualizations, it does not vouch for eliminating errors. Instead of dismissing uniqueness and individualization, accepting errors as human or system failures and seeking remedial measures would benefit forensic science practice and criminal investigation. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Reading and Spelling Skills in German Third Graders: Examining the Role of Student and Context Characteristics

ERIC Educational Resources Information Center

Suchodoletz, Antje; Larsen, Ross A. A.; Gunzenhauser, Catherine; Fäsche, Anika

2015-01-01

Background: Educational processes and outcomes are influenced by a multitude of factors, including individual and contextual characteristics. Recently, studies have demonstrated that student and context characteristics may produce unique and cumulative effects on educational outcomes. Aims: The study aimed to investigate (1) the relative…

The crystal structure of NADPH:ferredoxin reductase from Azotobacter vinelandii.

PubMed Central

Sridhar Prasad, G.; Kresge, N.; Muhlberg, A. B.; Shaw, A.; Jung, Y. S.; Burgess, B. K.; Stout, C. D.

1998-01-01

NADPH:ferredoxin reductase (AvFPR) is involved in the response to oxidative stress in Azotobacter vinelandii. The crystal structure of AvFPR has been determined at 2.0 A resolution. The polypeptide fold is homologous with six other oxidoreductases whose structures have been solved including Escherichia coli flavodoxin reductase (EcFldR) and spinach, and Anabaena ferredoxin:NADP+ reductases (FNR). AvFPR is overall most homologous to EcFldR. The structure is comprised of a N-terminal six-stranded antiparallel beta-barrel domain, which binds FAD, and a C-terminal five-stranded parallel beta-sheet domain, which binds NADPH/NADP+ and has a classical nucleotide binding fold. The two domains associate to form a deep cleft where the NADPH and FAD binding sites are juxtaposed. The structure displays sequence conserved motifs in the region surrounding the two dinucleotide binding sites, which are characteristic of the homologous enzymes. The folded over conformation of FAD in AvFPR is similar to that in EcFldR due to stacking of Phe255 on the adenine ring of FAD, but it differs from that in the FNR enzymes, which lack a homologous aromatic residue. The structure of AvFPR displays three unique features in the environment of the bound FAD. Two features may affect the rate of reduction of FAD: the absence of an aromatic residue stacked on the isoalloxazine ring in the NADPH binding site; and the interaction of a carbonyl group with N10 of the flavin. Both of these features are due to the substitution of a conserved C-terminal tyrosine residue with alanine (Ala254) in AvFPR. An additional unique feature may affect the interaction of AvFPR with its redox partner ferredoxin I (FdI). This is the extension of the C-terminus by three residues relative to EcFldR and by four residues relative to FNR. The C-terminal residue, Lys258, interacts with the AMP phosphate of FAD. Consequently, both phosphate groups are paired with a basic group due to the simultaneous interaction of the FMN phosphate with Arg51 in a conserved FAD binding motif. The fourth feature, common to homologous oxidoreductases, is a concentration of 10 basic residues on the face of the protein surrounding the active site, in addition to Arg51 and Lys258. PMID:9865948

BEYOND THE INDICES: RELATIONS OF HABITAT AND FISH CHARACTERISTICS IN THE GEORGIA PIEDMONT

EPA Science Inventory

The Georgia Department of Natural Resources has conducted biological sampling at 180 stream sites in the Georgia Piedmont (1998-99) and recorded several trophic and abundance characteristics of the fish assemblages and habitat at each site. These characteristics were combined to ...

AmeriFlux US-MOz Missouri Ozark Site

DOE Data Explorer

Gu, Lianhong [Oak Ridge National Laboratory

2016-01-01

This is the AmeriFlux version of the carbon flux data for the site US-MOz Missouri Ozark Site. Site Description - The site is located in the University of Missouri Baskett Wildlife Research area, situated in the Ozark region of central Missouri. The site is uniquely located in the ecologically important transitional zone between the central hardwood region and the central grassland region of the US. The land has been publically owned since the 1930s, and is on a land tract that was forested with the same dominant species before settlement in the early 1800s.

Meeting the Needs of Students with Coexisting Visual Impairments and Learning Disabilities

ERIC Educational Resources Information Center

Jones, Beth A.; Hensley-Maloney, Lauren

2015-01-01

The coexistence of visual impairments and learning disabilities presents unique challenges. It is imperative that teachers be apprised of the characteristics of this population as well as instructional strategies targeted at meeting their unique needs. The authors highlight typical patterns of performance and provide suggestions for effective…

Problems and Prospects of SWAT Model Application on an Arid/Semi-arid Watershed in Arizona

EPA Science Inventory

Hydrological characteristics in the semi-arid southwest create unique challenges to watershed modelers. Streamflow in these regions is largely dependent on seasonal, short term, and high intensity rainfall events. The objectives of this study are: 1) to analyze the unique hydrolo...

Using Video in Higher Education. IET Paper on Broadcasting No. 243.

ERIC Educational Resources Information Center

Bates, A. W.

Television has unique teaching functions that are significant for university education, and new developments in technology enable television to overcome some of its previous difficulties and weaknesses. Television's presentational power gives it two unique teaching characteristics: its ability to provide learning materials otherwise unavailable to…

Problems and Prospects of Swat Model Application on an Arid/Semi-Arid Watershed in Arizona

EPA Science Inventory

Hydrological characteristics in the semi-arid southwest create unique challenges to watershed modellers. Streamflow in these regions is largely dependent on seasonal, short term, and high intensity rainfall events. The objectives of this study are: 1) to analyze the unique hydrol...

Predicting DNAPL Source Zone and Plume Response Using Site-Measured Characteristics

DTIC Science & Technology

2017-05-19

FINAL REPORT Predicting DNAPL Source Zone and Plume Response Using Site- Measured Characteristics SERDP Project ER-1613 MAY 2017...Final Report 3. DATES COVERED (From - To) 2007 - 2017 4. TITLE AND SUBTITLE PREDICTING DNAPL SOURCE ZONE AND PLUME RESPONSE USING SITE- MEASURED ...historical record of concentration and head measurements , particularly in the near-source region. For each site considered, currently available data

Patient characteristics, resource use and outcomes associated with general internal medicine hospital care: the General Medicine Inpatient Initiative (GEMINI) retrospective cohort study

PubMed Central

Verma, Amol A.; Guo, Yishan; Kwan, Janice L.; Lapointe-Shaw, Lauren; Rawal, Shail; Tang, Terence; Weinerman, Adina; Cram, Peter; Dhalla, Irfan A.; Hwang, Stephen W.; Laupacis, Andreas; Mamdani, Muhammad M.; Shadowitz, Steven; Upshur, Ross; Reid, Robert J.; Razak, Fahad

2017-01-01

Background: The precise scope of hospital care delivered under general internal medicine services remains poorly quantified. The purpose of this study was to describe the demographic characteristics, medical conditions, health outcomes and resource use of patients admitted to general internal medicine at 7 hospital sites in the Greater Toronto Area. Methods: This was a retrospective cohort study involving all patients who were admitted to or discharged from general internal medicine at the study sites between Apr. 1, 2010, and Mar. 31, 2015. Clinical data from hospital electronic information systems were linked to administrative data from each hospital. We examined trends in resource use and patient characteristics over the study period. Results: There were 136 208 admissions to general internal medicine involving 88 121 unique patients over the study period. General internal medicine admissions accounted for 38.8% of all admissions from the emergency department and 23.7% of all hospital bed-days. Over the study period, the number of admissions to general internal medicine increased by 32.4%; there was no meaningful change in the median length of stay or cost per hospital stay. The median patient age was 73 (interquartile range [IQR] 57-84) years, and the median number of coexisting conditions was 6 (IQR 3-9). The median acute length of stay was 4.6 (IQR 2.5-8.6) days, and the median total cost per hospital stay was $5850 (IQR $3915-$10 061). Patients received at least 1 computed tomography scan in 52.2% of admissions. The most common primary discharge diagnoses were pneumonia (5.0% of admissions), heart failure (4.7%), chronic obstructive pulmonary disease (4.1%), urinary tract infection (4.0%) and stroke (3.6%). Interpretation: Patients admitted to general internal medicine services represent a large, heterogeneous, resource-intensive and growing population. Understanding and improving general internal medicine care is essential to promote a high-quality, sustainable health care system. PMID:29237706

32 CFR 806.15 - FOIA exemptions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... safeguarding social security numbers (SSN). It states: “SSNs are personal and unique to each individual... accessible DoD web sites unless to do so is clearly authorized by law and implementing regulation and policy. Personal information should not be posted at nonpublicly accessible web sites unless it is mission...

Savannah River Site Robotics

ScienceCinema

None

2018-04-16

Meet Sandmantis and Frankie, two advanced robotic devices that are key to cleanup at Savannah River Site. Sandmantis cleans hard, residual waste off huge underground storage tanks. Frankie is equipped with unique satellite capabilities and sensing abilties that can determine what chemicals still reside in the tanks in a cost effective manner.

A new landscape classification system for monitoring and assessment of pastures

USDA-ARS?s Scientific Manuscript database

Pasturelands in the United States span a broad range of climate, soils, physical sites, and management. Rather than treat each site as a unique entity, this diversity must be classified into basic units for research and management purposes. A similar system based on ecological principles is needed f...

Savannah River Site Robotics

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

Meet Sandmantis and Frankie, two advanced robotic devices that are key to cleanup at Savannah River Site. Sandmantis cleans hard, residual waste off huge underground storage tanks. Frankie is equipped with unique satellite capabilities and sensing abilties that can determine what chemicals still reside in the tanks in a cost effective manner.

Landscapes, depositional environments and human occupation at Middle Paleolithic open-air sites in the southern Levant, with new insights from Nesher Ramla, Israel

NASA Astrophysics Data System (ADS)

Zaidner, Yossi; Frumkin, Amos; Friesem, David; Tsatskin, Alexander; Shahack-Gross, Ruth

2016-04-01

Middle Paleolithic human occupation in the Levant (250-50 ka ago) has been recorded in roofed (cave and rockshelter) and open-air sites. Research at these different types of sites yielded different perspectives on the Middle Paleolithic human behavior and evolution. Until recently, open-air Middle Paleolithic sites in the Levant were found in three major sedimentary environments: fluvial, lake-margin and spring. Here we describe a unique depositional environment and formation processes at the recently discovered open-air site of Nesher Ramla (Israel) and discuss their contribution to understanding site formation processes in open-air sites in the Levant. The site is 8-m-thick Middle Paleolithic sequence (OSL dated to 170-80 ka) that is located in a karst sinkhole formed by gravitational deformation and sagging into underground voids. The sedimentary sequence was shaped by gravitational collapse, cyclic colluviation of soil and gravel into the depression, waterlogging, in situ pedogenesis and human occupation. Original bedding and combustion features are well-preserved in the Lower archaeological sequence, a rare occurrence in comparison to other open-air archaeological sites. This phenomenon coincides with episodes of fast sedimentation/burial, which also allowed better preservation of microscopic remains such as ash. The Upper archaeological sequence does not exhibit bedding or preservation of ash, despite presence of heat-affected lithic artifacts, which makes it similar to other open-air sites in the Levant. We suggest that rate of burial is the major factor that caused the difference between the Upper and Lower sequences. The differences in the burial rate may be connected to environmental and vegetation changes at the end of MIS 6. We also identified an interplay between sediment in-wash and density of human activity remains, i.e. during episodes of low natural sediment input the density of artifacts is higher relative to episodes with high rate of sediment in-wash. The detailed analysis of natural and anthropogenic processes at Nesher Ramla suggests a much wider spectrum of processes than previously reported for southern Levantine Paleolithic sites. Nesher Ramla shares certain depositional and post-depositional characteristics with both cave and open-air sites and provides a better insight into processes which control both types of sites.

Selecting a Roof Membrane.

ERIC Educational Resources Information Center

Waldron, Larry W.

1990-01-01

Offers a brief synopsis of the unique characteristics of the following roof membranes: (1) built-up roofing; (2) elastoplastic membranes; (3) modified bitumen membranes; (4) liquid applied membranes; and (5) metal roofing. A chart compares the characteristics of the raw membranes only. (MLF)

The importance of scale-dependent ravine characteristics on breeding-site selection by the Burrowing Parrot, Cyanoliseus patagonus

PubMed Central

Rios, Rodrigo S.; Vargas-Rodriguez, Renzo; Novoa-Jerez, Jose-Enrique; Squeo, Francisco A.

2017-01-01

In birds, the environmental variables and intrinsic characteristics of the nest have important fitness consequences through its influence on the selection of nesting sites. However, the extent to which these variables interact with variables that operate at the landscape scale, and whether there is a hierarchy among the different scales that influences nest-site selection, is unknown. This interaction could be crucial in burrowing birds, which depend heavily on the availability of suitable nesting locations. One representative of this group is the burrowing parrot, Cyanoliseus patagonus that breeds on specific ravines and forms large breeding colonies. At a particular site, breeding aggregations require the concentration of adequate environmental elements for cavity nesting, which are provided by within ravine characteristics. Therefore, intrinsic ravine characteristics should be more important in determining nest site selection compared to landscape level characteristics. Here, we assess this hypothesis by comparing the importance of ravine characteristics operating at different scales on nest-site selection and their interrelation with reproductive success. We quantified 12 characteristics of 105 ravines in their reproductive habitat. For each ravine we quantified morphological variables, distance to resources and disturbance as well as nest number and egg production in order to compare selected and non-selected ravines and determine the interrelationship among variables in explaining ravine differences. In addition, the number of nests and egg production for each reproductive ravine was related to ravine characteristics to assess their relation to reproductive success. We found significant differences between non-reproductive and reproductive ravines in both intrinsic and extrinsic characteristics. The multidimensional environmental gradient of variation between ravines, however, shows that differences are mainly related to intrinsic morphological characteristics followed by extrinsic variables associated to human disturbance. Likewise, within reproductive ravines, intrinsic characteristics are more strongly related to the number of nests. The probability of producing eggs, however, was related only to distance to roads and human settlements. Patterns suggest that C. patagonus mainly selects nesting sites based on intrinsic morphological characteristics of ravines. Scale differences in the importance of ravine characteristics could be a consequence of the particular orography of the breeding habitat. The arrangement of resources is associated to the location of the gullies rather than to individual ravines, determining the spatial availability and disposition of resources and disturbances. Thus, nest selection is influenced by intrinsic characteristics that maximize the fitness of individuals. Scaling in nest-selection is discussed under an optimality approach that partitions patch selection based on foraging theory. PMID:28462019

10 CFR 960.5-2 - Technical guidelines.

Code of Federal Regulations, 2014 CFR

2014-01-01

..., operation, and closure. The first group includes conditions on population density and distribution, site... the surface characteristics of the site, the characteristics of the host rock and surrounding strata...

10 CFR 960.5-2 - Technical guidelines.

Code of Federal Regulations, 2011 CFR

2011-01-01

..., operation, and closure. The first group includes conditions on population density and distribution, site... the surface characteristics of the site, the characteristics of the host rock and surrounding strata...

10 CFR 960.5-2 - Technical guidelines.

Code of Federal Regulations, 2013 CFR

2013-01-01

..., operation, and closure. The first group includes conditions on population density and distribution, site... the surface characteristics of the site, the characteristics of the host rock and surrounding strata...

10 CFR 960.5-2 - Technical guidelines.

Code of Federal Regulations, 2012 CFR

2012-01-01

..., operation, and closure. The first group includes conditions on population density and distribution, site... the surface characteristics of the site, the characteristics of the host rock and surrounding strata...

Human embryonic stem cell phosphoproteome revealed by electron transfer dissociation tandem mass spectrometry.

PubMed

Swaney, Danielle L; Wenger, Craig D; Thomson, James A; Coon, Joshua J

2009-01-27

Protein phosphorylation is central to the understanding of cellular signaling, and cellular signaling is suggested to play a major role in the regulation of human embryonic stem (ES) cell pluripotency. Here, we describe the use of conventional tandem mass spectrometry-based sequencing technology--collision-activated dissociation (CAD)--and the more recently developed method electron transfer dissociation (ETD) to characterize the human ES cell phosphoproteome. In total, these experiments resulted in the identification of 11,995 unique phosphopeptides, corresponding to 10,844 nonredundant phosphorylation sites, at a 1% false discovery rate (FDR). Among these phosphorylation sites are 5 localized to 2 pluripotency critical transcription factors--OCT4 and SOX2. From these experiments, we conclude that ETD identifies a larger number of unique phosphopeptides than CAD (8,087 to 3,868), more frequently localizes the phosphorylation site to a specific residue (49.8% compared with 29.6%), and sequences whole classes of phosphopeptides previously unobserved.

Unique Rhizosphere Micro-characteristics Facilitate Phytoextraction of Multiple Metals in Soil by the Hyperaccumulating Plant Sedum alfredii.

PubMed

Hou, Dandi; Wang, Kai; Liu, Ting; Wang, Haixin; Lin, Zhi; Qian, Jie; Lu, Lingli; Tian, Shengke

2017-05-16

Understanding the strategies that the roots of hyperaccumulating plants use to extract heavy metals from soils is important for optimizing phytoremediation. The rhizosphere characteristics of Sedum alfredii, a hyperaccumulator, were investigated 6 months after it had been planted in weathered field soils contaminated with 5.8 μg of Cd g -1 , 1985.1 μg of Zn g -1 , 667.5 μg of Pb g -1 , and 698.8 μg of Cu g -1 . In contrast with the non-hyperaccumulating ecotype (NHE), the hyperaccumulating ecotype (HE) of S. alfredii was more tolerant to the metals, and higher levels of Cd and Zn accumulated. The HE was characterized by a unique rhizosphere, including extensive root systems, a reduced soil pH, a higher metal bioavailability, and increased rhizomicrobial activity. The bioavailability of metals was significantly correlated with the HE's unique bacterial communities (P < 0.005). The HE harbored abundant Streptomyces (9.43%, family Streptomycetaceae), Kribbella (1.08%, family Nocardioidaceae), and an unclassified genus (1.09%, family Nocardioidaceae) in its rhizosphere, a composition that differed from that of the NHE. PICRUSt analysis predicted high relative abundances of imputed functional profiles in the HE rhizosphere related to membrane transport and amino acid metabolism. This study reveals the rhizosphere characteristics, particularly the unique bacterial rhizobiome of a hyperaccumulator, that might provide a new approach to facilitating heavy metal phytoextraction.

The permuted generator hypothesis for the origin of a genetic code

NASA Technical Reports Server (NTRS)

Folsome, C.

1977-01-01

Protocells had no known means of ensuring that their randomly collected proteins would be duplicated. A possible, albeit inexact, mechanism for protein synthesis in a primitive t-RNA is presented, whereby an oligonucleotide (12 units) in a circular configuration is able to align a generator site with amino acid discriminator sites. In this way, unique anticodons could be specified for each site and replication could occur.

Denitrification and Phosphorus Sequestration in Restored Oyster Beds in the Indian River Lagoon, Florida, USA

NASA Astrophysics Data System (ADS)

Gallagher, S. M.; Schmidt, C. A.; Walters, L.

2016-12-01

In 2016, an algae bloom in the St. Lucie River in Florida led the governor to declare a state of emergency. The river is part of a connected system of estuaries along the Atlantic coast of Florida called the Indian River Lagoon (IRL). As with many estuaries around the world, nutrient loading in the IRL has led to periodic eutrophication. As a result, much research has been done to address nutrients in these systems. Previous estuary studies have related oyster restoration to denitrification and phosphorus sequestration in their bed sediment. To this point, these studies have been inconclusive, and have only focused on seasonal variation in nutrient cycling. In 2007, yearly oyster bed installation and restoration began in a study area in the IRL. By 2016, beds aged up to eleven years were available for sampling. This unique advantage allowed investigation of bed sediment and nutrient cycling over long periods of time. Sediment from the IRL was measured for organic matter, microbial weight, carbon, nitrogen, and phosphorus. Denitrification was measured using an acetylene block technique. A statistical analysis was used to find differences in sediment characteristics and denitrification between restored beds and control sites over time. In addition, sequencing of 16S rRNA DNA and a variety of denitrifying genes was used to identify bacterial species and their denitrifying capability in the sediment. The ability to sequence denitrification genes in established oyster beds over a period of years was also unique to this study. Significant differences were found in soil properties, denitrification rates, and phosphorus sequestration between control sites and restored oyster beds. Gene sequencing also found differences in bacterial populations between the sites. Oyster bed restoration resulted in a rapid increase in nutrient removal as beds developed over three years, but additional benefits were limited as restoration progressed further. This study adds an investigation of IRL oysters to existing knowledge of nutrient removal by oysters in other estuaries. These results help clarify single year studies focused on seasonal changes by showing a rapid increase in oyster bed nutrient removal over a period of three years.

Design and Field Test of a Mass Efficient Crane for Lunar Payload Handling and Inspection: The Lunar Surface Manipulation System

NASA Technical Reports Server (NTRS)

Doggett, William R.; King, Bruce D.; Jones, Thomas Carno; Dorsey, John T.; Mikulas, Martin M.

2008-01-01

Devices for lifting, translating and precisely placing payloads are critical for efficient Earthbased construction operations. Both recent and past studies have demonstrated that devices with similar functionality will be needed to support lunar outpost operations. Lunar payloads include: a) prepackaged hardware and supplies which must be unloaded from landers and then accurately located at their operational site, b) sensor packages used for periodic inspection of landers, habitat surfaces, etc., and c) local materials such as regolith which require grading, excavation and placement. Although several designs have been developed for Earth based applications, these devices lack unique design characteristics necessary for transport to and use on the harsh lunar surface. These design characteristics include: a) composite components, b) compact packaging for launch, c) simple in-field reconfiguration and repair, and d) support for tele-operated or automated operations. Also, in contrast to Earth-based construction, where special purpose devices dominate a construction site, a lunar outpost will require versatile devices which provide operational benefit from initial construction through sustained operations. This paper will detail the design of a unique, high performance, versatile lifting device designed for operations on the lunar surface. The device is called the Lunar Surface Manipulation System to highlight the versatile nature of the device which supports conventional cable suspended crane operations as well as operations usually associated with a manipulator such as precise positioning where the payload is rigidly grappled by a tool attached to the tip of the device. A first generation test-bed to verify design methods and operational procedures is under development at the NASA Langley Research Center and recently completed field tests at Moses Lake Washington. The design relied on non-linear finite element analysis which is shown to correlate favorably with laboratory experiments. A key design objective, reviewed in this paper, is the device s simplicity, resulting from a focus on the minimum set of functions necessary to perform payload offload. Further development of the device has the potential for significant mass savings, with a high performance device incorporating composite elements estimated to have a mass less than 3% of the mass of the maximum lunar payload lifted at the tip. The paper will conclude with future plans for expanding the operational versatility of the device.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kim, Hyun You; Liu, Ping

Mixed metal oxides have attracted considerable attention in heterogeneous catalysis due to the unique stability, reactivity, and selectivity. Here, the activity and stability of the CuTiO x monolayer film supported on Cu(111), CuTiO x/Cu(111), during CO oxidation was explored using density functional theory (DFT). The unique structural frame of CuTiO x is able to stabilize and isolate a single Cu + site on the terrace, which is previously proposed active for CO oxidation. Furthermore, it is not the case, where the reaction via both the Langmuir–Hinshelwood (LH) and the Mars-van Krevelen (M-vK) mechanisms are hindered on such single Cu +more » site. Upon the formation of step-edges, the synergy among Cu δ+ sites, TiO x matrix, and Cu(111) is able to catalyze the reaction well. Depending on temperatures and partial pressure of CO and O 2, the surface structure varies, which determines the dominant mechanism. In accordance with our results, the Cu δ+ ion alone does not work well for CO oxidation in the form of single sites, while the synergy among multiple active sites is necessary to facilitate the reaction.« less

Determination of solute site occupancies within γ' precipitates in nickel-base superalloys via orientation-specific atom probe tomography

DOE PAGES

Meher, Subhashish; Rojhirunsakool, Tanaporn; Nandwana, Peeyush; ...

2015-04-28

In this study, the analytical limitations in atom probe tomography such as resolving a desired set of atomic planes, for solving complex materials science problems, have been overcome by employing a well-developed unique and reproducible crystallographic technique, involving synergetic coupling of orientation microscopy with atom probe tomography. The crystallographic information in atom probe reconstructions has been utilized to determine the solute site occupancies in Ni-Al-Cr based superalloys accurately. The structural information in atom probe reveals that both Al and Cr occupy the same sub-lattice within the L1 2-ordered g precipitates to form Ni 3(Al,Cr) precipitates in a Ni-14Al-7Cr(at.%) alloy. Interestingly,more » the addition of Co, which is a solid solution strengthener, to a Ni-14Al-7Cr alloy results in the partial reversal of Al site occupancy within g precipitates to form (Ni,Al) 3(Al,Cr,Co) precipitates. This unique evidence of reversal of Al site occupancy, resulting from the introduction of other solutes within the ordered structures, gives insights into the relative energetics of different sub-lattice sites when occupied by different solutes.« less

CDC/NACCHO Accreditation Support Initiative: advancing readiness for local and tribal health department accreditation.

PubMed

Monteiro, Erinn; Fisher, Jessica Solomon; Daub, Teresa; Zamperetti, Michelle Chuk

2014-01-01

Health departments have various unique needs that must be addressed in preparing for national accreditation. These needs require time and resources, shortages that many health departments face. The Accreditation Support Initiative's goal was to test the assumption that even small amounts of dedicated funding can help health departments make important progress in their readiness to apply for and achieve accreditation. Participating sites' scopes of work were unique to the needs of each site and based on the proposed activities outlined in their applications. Deliverables and various sources of data were collected from sites throughout the project period (December 2011-May 2012). Awardees included 1 tribal and 12 local health departments, as well as 5 organizations supporting the readiness of local and tribal health departments. Sites dedicated their funding toward staff time, accreditation fees, completion of documentation, and other accreditation readiness needs and produced a number of deliverables and example documents. All sites indicated that they made accreditation readiness gains that would not have occurred without this funding. Preliminary evaluation data from the first year of the Accreditation Support Initiative indicate that flexible funding arrangements may be an effective way to increase health departments' accreditation readiness.

Complex catalytic behaviors of CuTiO x mixed-oxide during CO oxidation

DOE PAGES

Kim, Hyun You; Liu, Ping

2015-09-21

Mixed metal oxides have attracted considerable attention in heterogeneous catalysis due to the unique stability, reactivity, and selectivity. Here, the activity and stability of the CuTiO x monolayer film supported on Cu(111), CuTiO x/Cu(111), during CO oxidation was explored using density functional theory (DFT). The unique structural frame of CuTiO x is able to stabilize and isolate a single Cu + site on the terrace, which is previously proposed active for CO oxidation. Furthermore, it is not the case, where the reaction via both the Langmuir–Hinshelwood (LH) and the Mars-van Krevelen (M-vK) mechanisms are hindered on such single Cu +more » site. Upon the formation of step-edges, the synergy among Cu δ+ sites, TiO x matrix, and Cu(111) is able to catalyze the reaction well. Depending on temperatures and partial pressure of CO and O 2, the surface structure varies, which determines the dominant mechanism. In accordance with our results, the Cu δ+ ion alone does not work well for CO oxidation in the form of single sites, while the synergy among multiple active sites is necessary to facilitate the reaction.« less

Ali Observatory in Tibet: a unique northern site for future CMB ground-based observations

NASA Astrophysics Data System (ADS)

Su, Meng

2015-08-01

Ground-based CMB observations have been performed at the South Pole and the Atacama desert in Chile. However, a significant fraction of the sky can not be observed from just these two sites. For a full sky coverage from the ground in the future, a northern site for CMB observation, in particular CMB polarization, is required. Besides the long-thought site in Greenland, the high altitude Tibet plateau provides another opportunity. I will describe the Ali Observatory in Tibet, located at N32°19', E80°01', as a potential site for ground-based CMB observations. The new site is located on almost 5100m mountain, near Gar town, where is an excellent site for both infrared and submillimeter observations. Study with the long-term database of ground weather stations and archival satellite data has been performed. The site has enough relative height on the plateau and is accessible by car. The Shiquanhe town is 40 mins away by driving, and a recently opened airport with 40 mins driving, the site also has road excess, electricity, and optical fiber with fast internet. Preliminary measurement of the Precipitable Water Vapor is ~one quarter less than 0.5mm per year and the long term monitoring is under development. In addition, surrounding higher sites are also available and could be further developed if necessary. Ali provides unique northern sky coverage and together with the South Pole and the Atacama desert, future CMB observations will be able to cover the full sky from ground.

Droppin' Knowledge: Black Women's Communication and Informal Learning in an Online Community

ERIC Educational Resources Information Center

Steptoe, Leslye Carynn

2011-01-01

The experiences of black women offer a unique perspective on how life is lived at the juncture of race and gender in the United States. This case study of an online community for black women centers on the site's potentiality as an online learning community as well as a uniquely black woman's space. It also explores interrelated aspects of…

Grooker, KartOO, Addict-o-Matic and More: Really Different Search Engines

ERIC Educational Resources Information Center

Descy, Don E.

2009-01-01

There are hundreds of unique search engines in the United States and thousands of unique search engines around the world. If people get into search engines designed just to search particular web sites, the number is in the hundreds of thousands. This article looks at: (1) clustering search engines, such as KartOO (www.kartoo.com) and Grokker…

Soils

Treesearch

John R. Jones; Norbert V. DeByle

1985-01-01

Edaphic and climatic characteristics of a site quite well define the quality of that site for plant growth. The importance of soil characteristics to the growth and well-being of aspen in the West is apparent from observations by many authors, from inferences resulting from work with other trees and agricultural crops, and from detailed study of aspen soils and site...

What users want in e-commerce design: effects of age, education and income.

PubMed

Lightner, Nancy J

2003-01-15

Preferences for certain characteristics of an online shopping experience may be related to demographic data. This paper discusses the characteristics of that experience, demographic data and preferences by demographic group. The results of an online survey of 488 individuals in the United States indicate that respondents are generally satisfied with their online shopping experiences, with security, information quality and information quantity ranking first in importance overall. The sensory impact of a site ranked last overall of the seven characteristics measured. Preferences for these characteristics in e-commerce sites were differentiated by age, education and income. The sensory impact of sites became less important as respondents increased in age, income or education. As the income of respondents increased, the importance of the reputation of the vendor rose. Web site designers may incorporate these findings into the design of e-commerce sites in an attempt to increase the shopping satisfaction of their users. Results from the customer relationship management portion of the survey suggest that current push technologies and site personalization are not an effective means of achieving user satisfaction.

Cloning of Pf3, a filamentous bacteriophage of Pseudomonas aeruginosa, into the pBD214 vector of Bacillus subtilis.

PubMed Central

Putterman, D G; Gryczan, T J; Dubnau, D; Day, L A

1983-01-01

The genome of Pf3, a filamentous single-stranded DNA bacteriophage of Pseudomonas aeruginosa (a gram-negative organism) was cloned into pBD214, a plasmid cloning vector of Bacillus subtilis (a gram-positive organism). Cloning in the gram-positive organism was done to avoid anticipated lethal effects. The entire Pf3 genome was inserted in each orientation at a unique Bc/I site within a thymidylate synthetase gene (from B. subtilis phage beta 22) on the plasmid. Additional clones were made by inserting EcoRI fragments of Pf3 DNA into a unique EcoRI site within this gene. Images PMID:6306273

Electronic-nose applications for fruit identification, ripeness, and quality grading

Treesearch

Manuela Baietto; Dan Wilson

2015-01-01

Fruits produce a wide range of volatile organic compounds that impart their characteristically distinct aromas and contribute to unique flavor characteristics. Fruit aroma and flavor characteristics are of key importance in determining consumer acceptance in commercial fruit markets based on individual preference. Fruit producers, suppliers and retailers traditionally...

Cleopatra's Egypt. A Museum Exhibition Explores Egypt during the Age of the Ptolemies.

ERIC Educational Resources Information Center

Bianchi, Robert S.

1989-01-01

Describes the historical premises behind a traveling exhibit of Egyptian Hellenistic art from the Ptolemic Period. Focuses on the uniquely Egyptian characteristics of this art, including religious symbolism, costume elements, and characteristics of craftsmanship. (LS)

Who is looking for an internship and successful in obtaining one? Examining application data from REU programs funded through NSF GEO

NASA Astrophysics Data System (ADS)

Hubenthal, M.; Kelly, M.

2017-12-01

The Directorate for Geosciences (GEO) at the National Science Foundation (NSF) is currently funding 60 Research Experiences for Undergraduate (REU) sites. Each site offers opportunities for 8 to 12 undergraduates to participate in research within solid earth, oceans, atmospheric and cryosphere sciences. Because applicant data is collected at individual REU sites, the exact number of unique applicants to all REU sites, and the demographics of this national applicant pool has not been previously reported. While some sites do provide some of this information to NSF in annual reports, obtaining and combining such data is problematic because the percentage of individuals that apply to multiple programs is unknown and generally believed anecdotally to be high, especially for students traditionally underrepresented in the geosciences. Understanding both the scale and makeup of the national applicant pool is important for several reasons. First, very little is known about how the supply and geographic location of slots in REU programs compares to the demand from undergraduate STEM majors interested in research experiences. Second, research into internship programs and their role in the career development process are limited by a lack of baseline data that includes both successful and unsuccessful internship applicants across the various sub-disciplines of the Earth sciences. Finally, designing and refining efforts to engage underrepresented populations in STEM research, and measuring the impact of such efforts is difficult without baseline data for comparison. We will present aggregate application data from up to 20 GEO REU funded programs. These programs represent Oceans, Atmospheres and Earth Science research areas and includes over a thousand applicants. Preliminary analysis suggests the number of unique applicants in the pool is higher than anecdotally predicted. Similarly, unique applicants from underrepresented communities also appears higher than anticipated.

[Adsorption of heavy metals on the surface of birnessite relationship with its Mn average oxidation state and adsorption sites].

PubMed

Wang, Yan; Tan, Wen-Feng; Feng, Xiong-Han; Qiu, Guo-Hong; Liu, Fan

2011-10-01

Adsorption characteristics of mineral surface for heavy metal ions are largely determined by the type and amount of surface adsorption sites. However, the effects of substructure variance in manganese oxide on the adsorption sites and adsorption characteristics remain unclear. Adsorption experiments and powder X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) were combined to examine the adsorption characteristics of Pb2+, Cu2+, Zn2+ and Cd2+ sequestration by birnessites with different Mn average oxidation state (AOS), and the Mn AOS dependent adsorption sites and adsorption characteristics. The results show that the maximum adsorption capacity of Pb2+, Cu2+, Zn2+ and Cd2+ increased with increasing birnessite Mn AOS. The adsorption capacity followed the order of Pb2+ > Cu2+ > Zn2+ > Cd2+. The observations suggest that there exist two sites on the surface of birnessite, i. e., high-binding-energy site (HBE site) and low-binding-energy site (LBE site). With the increase of Mn AOS for birnessites, the amount of HBE sites for heavy metal ions adsorption remarkably increased. On the other hand, variation in the amount of LBE sites was insignificant. The amount of LBE sites is much more than those of HBE sites on the surface of birnessite with low Mn AOS. Nevertheless, both amounts on the surface of birnessite with high Mn AOS are very close to each other. Therefore, the heavy metal ions adsorption capacity on birnessite is largely determined by the amount of HBE sites. On birnessite surface, adsorption of Cu2+, Zn2+, and Cd2+ mostly occurred at HBE sites. In comparison with Zn2+ and Cd2+, more Cu2+ adsorbed on the LBW sites. Pb2+ adsorption maybe occupy at both LBE sites and HBE sites simultaneously.

Advancing Sustainable Development through Understanding the Coastal Environment: The Garolim Bay in the West Coast of Korea.

NASA Astrophysics Data System (ADS)

Park, M. J.

2017-12-01

The coastal environment worldwide is increasingly more populated and developed in recent years. As a result, the natural coastal environment which often provides habitats for various plants and animals and nursery grounds for fishes has become scarce. However, it is well-known that the natural coastal environment is important for the well-being of the ecosystem including human. In addition, it may contribute to the local economy by attracting many visitors who like to appreciate and enjoy the nature. Although there may be efforts to preserve the natural coastal environment by governments and environmental groups, there may be also needs to develop the coastal environment. In order to preserve the natural environment without sacrificing the local economy, it would be important to appreciate the unique characteristics of the coastal environment that may contribute to attracting many visitors. In this study, we discuss some unique characteristics of the coastal environment located in the west coast of Korea facing the eastern Yellow Sea. The Garolim Bay is a semi-enclosed bay with the narrow opening of about 2 km and the length of about 20 km, and has a spring tidal range over 6 m. It is well known for vast tidal flats and healthy ecosystems that supports high productive and diverse marine lives including spotted seals. The tidal wave propagates north along the eastern Yellow Sea and currents become strong off the Garolim Bay due to the promontory effect and mixing of the water column results in cooling of surface water. Due to its characteristic offshore environment, narrow opening and abrupt turning over 120 degrees in the midst of acceleration of strong currents, the Garolim Bay presents the unique physical and geological environment resulting in characteristic physical, biological and geological features. In fact, the name of the bay `Garolim' meaning 'Dew-induced Forest', may have resulted from these unique physical and geological characteristics of the bay.

Examining the Development of Self-Authorship among Student Veterans

ERIC Educational Resources Information Center

Stone, Sharon L. M.

2014-01-01

The literature has shown that student veterans arrive in college with unique characteristics and also face unique challenges (Black et al., 2007; Bonar & Domenici, 2011; Church, 2009; DiRamio & Jarvis, 2011). There is also some evidence that student veterans develop complex ways of making meaning at younger ages than students in the…

Withstanding a record drought: Dry season sap flow and safety margins of canopy epiphytes in three sites along an elevation gradient in a tropical montane cloud forest

NASA Astrophysics Data System (ADS)

Gotsch, S. G.; Darby, A.; Glunk, A.; Murray, J.; Draguljic, D.

2016-12-01

Tropical montane cloud forests (TMCFs) are projected to experience shifts in microclimate due to changes in precipitation patterns and cloud base heights. Understanding how the TMCF will be affected by such changes is critical since these forests harbor a great number of endemic species and the intact forest plays an important role in local and regional hydrology. The epiphyte community is one of the characteristic components of this unique ecosystem. Epiphytes contribute greatly to the biomass and diversity of the forest, and play an important role in the TMCF water cycle. The ecosystem role played by the epiphyte community may be lost if these species, which lack roots to the ground, cannot withstand projected changes in climate. We measured dry season sap flow in canopy epiphytes in three sites along an elevation gradient, in Monteverde Costa Rica during an extreme drought event. In addition, we measured predawn and midday leaf water potentials, and dry season pressure-volume curves on focal species. We found that during a month-long dry period, species in all sites reduced transpiration considerably, and for a two-week period, sap flow rates were close to zero. During this time, predawn and midday leaf water potentials remained high (> -1.0 MPa) due to the utilization of stored leaf water and strong stomatal regulation. At the end of the dry season, midday leaf water potentials were approximately 2x lower in the driest versus the wettest site (AVG: -0.94 vs. -0.47 MPa) although hydraulic safety margins were greater at the driest sites. Our results indicate that epiphyte communities vary in their water use strategies and that the epiphyte community in wetter sites is more likely to experience hydraulic failure if there are increases in the number of days without precipitation and/or increases in temperature.

HiRISE observations of Recurring Slope Lineae (RSL) during southern summer on Mars

USGS Publications Warehouse

Ojha, Lujendra; McEwen, Alfred; Dundas, Colin; Byrne, Shane; Mattson, Sarah; Wray, James; Masse, Marion; Schaefer, Ethan

2014-01-01

Recurring Slope Lineae (RSL) are active features on Mars that might require flowing water. Most examples observed through 2011 formed on steep, equator-facing slopes in the southern mid-latitudes. They form and grow during warm seasons and fade and often completely disappear during colder seasons, but recur over multiple Mars years. They are recognizable by their incremental growth, relatively low albedo and downhill orientation. We examined all images acquired by HiRISE during Ls 250–10° (slightly longer than southern summer, Ls 270–360°) of Mars years 30–31 (03/2011–10/2011), and supplemented our results with data from previous studies to better understand the geologic context and characteristics of RSL. We also confirmed candidate and likely sites from previous studies and discovered new RSL sites. We report 13 confirmed RSL sites, including the 7 in McEwen et al. (McEwen et al. [2011]. Science 333(6043), 740–743]. The observed seasonality, latitudinal and slope orientation preferences, and THEMIS bright- ness temperatures indicate that RSL require warm temperatures to form. We conclude that RSL are a unique phenomenon on Mars, clearly distinct from other slope processes that occur at high latitudes associated with seasonal CO2 frost, and episodic mass wasting on equatorial slopes. However, only 41% (82 out of 200) of the sites that present apparently suitable conditions for RSL formation (steep, equator-facing rocky slopes with bedrock exposure) in the southern mid-latitudes (28–60°S) contain any candidate RSL, with confirmed RSL present only in 7% (13 sites) of those locations. Significant variability in abundance, size and exact location of RSL is also observed at most sites, indicating additional controls such as availability of water or salts that might be playing a crucial role.

Variation in characteristics of ambient particulate matter at eight locations in the Netherlands - The RAPTES project

NASA Astrophysics Data System (ADS)

Strak, Maciej; Steenhof, Maaike; Godri, Krystal J.; Gosens, Ilse; Mudway, Ian S.; Cassee, Flemming R.; Lebret, Erik; Brunekreef, Bert; Kelly, Frank J.; Harrison, Roy M.; Hoek, Gerard; Janssen, Nicole A. H.

2011-08-01

Numerous epidemiological studies have shown health effects related to short- and long-term exposure to elevated levels of ambient particulate matter (PM). It is not clear however which specific characteristics (e.g., size, components) or sources of PM are responsible for the observed effects. The aim of RAPTES (Risk of Airborne Particles: a Toxicological-Epidemiological hybrid Study) was to investigate which specific physical, chemical or oxidative characteristics of ambient PM are associated with adverse effects of PM on health. This was done by performing experimental exposure of human volunteers to air pollution at several real-world settings that had high contrast and low correlation between several PM characteristics. For this goal, eight sites in the Netherlands that differed in local PM emission sources were chosen for extensive air pollution characterization. Measurement sites included an underground train station, three different road traffic sites, an animal farm, a sea harbor, a site located in the vicinity of steelworks, and an urban background site. Five- to six-hours average concentration measurements at each site were made between June 2007 and October 2009. We measured PM 10, PM 2.5, particle number concentration (PNC), oxidative potential of PM, absorbance, endotoxin content, as well as elemental and chemical composition of PM, and gaseous pollutants concentrations. This paper presents a detailed characterization of particulate air pollution at the sampling sites. We found significant differences in all PM characteristics between the sites. The underground train station, compared to each outdoor location, had substantially higher concentrations of nearly all PM characteristics. The average PM 10 and PM 2.5 mass concentrations at the underground train station were 394 μg m -3 and 137 μg m -3, respectively, which was 14.1 and 7.6 times higher than the urban background. The sum of the concentrations of trace metals in fine and coarse PM was nearly 20 times above the outdoor levels. Elemental carbon (EC) was elevated at the underground site in the fine but also in the coarse mode, in contrast to the traffic sites where EC was predominantly found in fine PM. The highest concentrations and contrasts in PNC were at the traffic sites (between 45,000 and 80,000 particles cm -3), which was several times higher than measured at any other site. Correlations of PNC with metals, PM 10, PM 2.5 and absorbance were low to moderate, while correlations between PM 10, PM 2.5 and the metals Cu and Fe were high. After excluding the underground train station data, correlations between PM10, EC and metals decreased whereas the correlation between PNC and EC increased. We conclude that we were able to successfully identify and characterize real-world situations with very different particle characteristics. High contrast and low correlations between PM characteristics, as well as consistency of these differences across sampling campaigns, provide a good basis for identifying health relevant PM characteristics in the upcoming analysis.

Combined estimation of kappa and shear-wave velocity profile of the Japanese rock reference

NASA Astrophysics Data System (ADS)

Poggi, Valerio; Edwards, Benjamin; Fäh, Donat

2013-04-01

The definition of a common soil or rock reference is a key issue in probabilistic seismic hazard analysis (PSHA), microzonation studies, local site-response analysis and, more generally, when predicted or observed ground motion is compared for sites of different characteristics. A scaling procedure, which accounts for a common reference, is then necessary to avoid bias induced by the differences in the local geology. Nowadays methods requiring the definition of a reference condition generally prescribe the characteristic of a rock reference, calibrated using indirect estimation methods based on geology or on surface proxies. In most cases, a unique average shear-wave velocity value is prescribed (e.g. Vs30 = 800m/s as for class A of the EUROCODE8). Some attempts at defining the whole shape of a reference rock velocity profile have been described, often without a clear physical justification of how such a selection was performed. Moreover, in spite of its relevance in affecting the high-frequency part of the spectrum, the definition of the associated reference attenuation is in most cases missing or, when present, still remains quite uncertain. In this study we propose an approach that is based on the comparison between empirical anelastic amplification functions from spectral modeling of earthquakes and average S-wave velocities computed using the quarter-wavelength approach. The method is an extension of the approach originally proposed by Poggi et al. (2011) for Switzerland, and is here applied to Japan. For the analysis we make use of a selection of 36 stiff-soil and rock sites from the Japanese KiK-net network, for which a measured velocity profile is available. With respect to the previous study, however, we now analyze separately the elastic and anelastic contributions of the estimated empirical amplification. In a first step - which is consistent with the original work - only the elastic part of the amplification spectrum is considered. This procedure allows the retrieval of the shape of the velocity profile that is characterized by no relative amplification within the network. Subsequently, the contribution of intrinsic attenuation is analyzed, disaggregated from the anelastic function by using the frequency independent (and site-dependent) attenuation operator kappa (κ). By comparing the dependency of κ with the quarter-wavelength velocity at selected sites, a frequency-dependent predictive equation is established to model the attenuation characteristics of an arbitrary rock or stiff-soil velocity model, such as the reference model obtained in the first step. The result of this application can be used to model the site-dependent attenuation for any rock and stiff-soil site for which an estimation of the velocity profile or its corresponding quarter-wavelength velocity representation is available. As an additional output of the present study, we also propose a simplified method to estimate kappa from the average velocity estimates over the first 30m (Vs30). We provide an example of such predictions for a range of Vs30 velocities up to 2000m/s.

Use of biological characteristics of common carp (Cyprinus carpio) to indicate exposure to hormonally active agents in selected Minnesota streams, 1999

USGS Publications Warehouse

Lee, Kathy E.; Blazer, Vicki; Denslow, Nancy D.; Goldstein, Robert M.; Talmage, Philip J.

2000-01-01

The presence of HAAs in selected Minnesota streams was indicated by biological characteristics in common carp. Biological characteristics used in this study identified WWTP effluent as a potential source of HAAs. Additionally, fish located at sites upstream of WWTP effluent primarily draining agricultural land show indications of HAAs, which may be the result of agricultural runoff or other sources of HAAs. There was variability among all sites and among sites within each site group. Differences among sites may be due to differences in water chemistry or fish exposure time. Natural variation in the biological characteristics may account for some of the differences observed in this study. This study and others indicate the presence of HAAs in surface water and the potential signs of endocrine disruption in resident fish populations. Detailed controlled studies could confirm the effects of particular chemicals such as pesticides or components of WWTPs on fish reproduction and population structure.

The Classroom Environment Questionnaire (CEQ): Development and preliminary structural validity.

PubMed

Lyons, Carissa; Brown, Ted; Bourke-Taylor, Helen

2018-04-16

Occupational therapists offer a unique perspective regarding the contribution of the environment to occupational performance. Therefore, a scale that measures the unique characteristics of the primary school classroom environment where children complete their daily schoolwork occupations is needed. The aim of this study was to develop and psychometrically evaluate a new teacher-report questionnaire that measures a number of environmental characteristics of primary school classrooms. Participants (N = 117) completed the Classroom Environment Questionnaire (CEQ), which utilises a 4-point Likert scale where teachers rate 51 environmental characteristics of their classroom. Teachers also rate the extent to which they believe the physical, social, temporal, institutional and cultural classroom environmental domains contribute to students' schoolwork performance using a 10-point scale. The structural validity of the CEQ was examined using principal component analysis (PCA). Inter-item correlations were examined using Pearson r correlations, while the internal consistency of the CEQ was assessed using Cronbach's alpha. PCA revealed the CEQ to be multidimensional, with 31 items loading onto nine viable factors, representing the unique nature of classroom environments. Based on the PCA results, 20 items were removed from the CEQ. Cronbach's alpha and correlation analysis indicated that most CEQ subsections had acceptable internal consistency (alpha range 0.70-0.82), with four subsections demonstrating a lower level of internal consistency (alpha range 0.55-0.69). Preliminary structural validity and internal consistency analysis findings confirm that the CEQ has potential to be a useful scale for professionals wishing to examine the unique characteristics of primary school classrooms that influence the occupational performance of students. Ongoing analyses will be undertaken to further explore the CEQ's validity and reliability. © 2018 Occupational Therapy Australia.

CHARACTERIZATION OF LARGE PARTICLES AT A RURAL SITE IN THE EASTERN UNITED STATES: MASS DISTRIBUTION AND INDIVIDUAL PARTICLE ANALYSIS

EPA Science Inventory

A unique combination of an effective sampler and analysis of individual particles has been used in studying large particles (> 5 micrometers) at a rural site in Eastern United States. The sampler is a modified 'high volume' rotary inertial impactor, which consists of four collect...

Off-Site Storage and Special Collections: A Study in Use and Impact in ARL Libraries in the United States

ERIC Educational Resources Information Center

Priddle, Charlotte; McCann, Laura

2015-01-01

Special collections libraries collect and preserve materials of intellectual and cultural heritage, providing access to unique research resources. As their holdings continue to expand, special collections in research libraries confront increased space pressures. Off-site storage facilities, used frequently by research libraries for general…

Top Ten Reasons to Seek Professional Challenges

ERIC Educational Resources Information Center

Salsberry, Trudy

2006-01-01

For three weeks, this author served as a stateside evaluator conducting school site visits at four Department of Defense Dependents Schools (DODDs) located in The Netherlands. Those three weeks in The Netherlands were filled with forming new relationships with team members at each school site, learning about the unique needs of children who are…

A Friend in Your Neighborhood: Local Risk Communication in a Technical Writing Classroom

ERIC Educational Resources Information Center

Rhodes, Lynne

2009-01-01

When examined rhetorically, Savannah River Site Community Preparedness Information calendars from 1994, 2004, and 2008 represent living rhetorical practices aimed at changing the public mind. My technical communication classroom at USC Aiken is uniquely situated for us to examine documents constantly generated by the site's Public Affairs…

Differential Transcription Factor Use by the KIR2DL4 Promoter Under Constitutive and IL-2/15-Treated Conditions

PubMed Central

Presnell, Steven R.; Zhang, Lei; Chlebowy, Corrin N.; Al-Attar, Ahmad; Lutz, Charles T.

2012-01-01

KIR2DL4 is unique among human KIR genes in expression, cellular localization, structure, and function, yet the transcription factors required for its expression have not been identified. Using mutagenesis, electrophoretic mobility shift assay, and co-transfection assays, we identified two redundant Runx binding sites in the 2DL4 promoter as essential for constitutive 2DL4 transcription, with contributions by a CRE site and initiator elements. IL-2-and IL-15-stimulated human NK cell lines increased 2DL4 promoter activity, which required functional Runx, CRE, and Ets sites. Chromatin immunoprecipitation experiments show that Runx3 and Ets1 bind the 2DL4 promoter in situ. 2DL4 promoter activity had similar transcription factor requirements in T cells. Runx, CRE, and Ets binding motifs are present in 2DL4 promoters from across primate species, but other postulated transcription factor binding sites are not preserved. Differences between 2DL4 and clonally-restricted KIR promoters suggest a model that explains the unique 2DL4 expression pattern in human NK cells. PMID:22467658

A unique H2A histone variant occupies the transcriptional start site of active genes.

PubMed

Soboleva, Tatiana A; Nekrasov, Maxim; Pahwa, Anuj; Williams, Rohan; Huttley, Gavin A; Tremethick, David J

2011-12-04

Transcriptional activation is controlled by chromatin, which needs to be unfolded and remodeled to ensure access to the transcription start site (TSS). However, the mechanisms that yield such an 'open' chromatin structure, and how these processes are coordinately regulated during differentiation, are poorly understood. We identify the mouse (Mus musculus) H2A histone variant H2A.Lap1 as a previously undescribed component of the TSS of active genes expressed during specific stages of spermatogenesis. This unique chromatin landscape also includes a second histone variant, H2A.Z. In the later stages of round spermatid development, H2A.Lap1 dynamically loads onto the inactive X chromosome, enabling the transcriptional activation of previously repressed genes. Mechanistically, we show that H2A.Lap1 imparts unique unfolding properties to chromatin. We therefore propose that H2A.Lap1 coordinately regulates gene expression by directly opening the chromatin structure of the TSS at genes regulated during spermatogenesis.

Kinetic Contributions to Gating by Interactions Unique to N-methyl-d-aspartate (NMDA) Receptors*

PubMed Central

Borschel, William F.; Cummings, Kirstie A.; Tindell, LeeAnn K.; Popescu, Gabriela K.

2015-01-01

Among glutamate-gated channels, NMDA receptors produce currents that subside with unusually slow kinetics, and this feature is essential to the physiology of central excitatory synapses. Relative to the homologous AMPA and kainate receptors, NMDA receptors have additional intersubunit contacts in the ligand binding domain that occur at both conserved and non-conserved sites. We examined GluN1/GluN2A single-channel currents with kinetic analyses and modeling to probe these class-specific intersubunit interactions for their role in glutamate binding and receptor gating. We found that substitutions that eliminate such interactions at non-conserved sites reduced stationary gating, accelerated deactivation, and imparted sensitivity to aniracetam, an AMPA receptor-selective positive modulator. Abolishing unique contacts at conserved sites also reduced stationary gating and accelerated deactivation. These results show that contacts specific to NMDA receptors, which brace the heterodimer interface within the ligand binding domain, stabilize actively gating receptor conformations and result in longer bursts and slower deactivations. They support the view that the strength of the heterodimer interface modulates gating in both NMDA and non-NMDA receptors and that unique interactions at this interface are responsible in part for basic differences between the kinetics of NMDA and non-NMDA currents at glutamatergic synapses. PMID:26370091

Hydrodynamic Impacts on Coastal Erosion and Deposition Processes in Cu Lao Dung (Soc Trang) and Rach Goc (Ca Mau)

NASA Astrophysics Data System (ADS)

Vo-Luong, H. P.; Phong, N. H.; Tran, D. X.; Ogston, A. S.

2016-02-01

Coastal mangrove forests are special and unique vegetation found in the tropics. They are classified as the most vulnerable ecosystem in coastal ecosystems. Moreover, mangrove forests are dynamic systems that directly influence coastal erosion and deposition processes. Cu Lao Dung (Soc Trang province) and Rach Goc (Ca Mau province) are chosen as studied sites. Although they both belong to the Mekong Delta on the eastern coast of Viet Nam, coastal erosion and deposition processes are different: Cu Lao Dung tends to be aggradational while the Rach Goc area is seriously erosional. This study aims to focus on the impact of hydrodynamics in the coastal processes at the Cu Lao Dung and Rach Goc sites. As part of field measurements in NE and SW monsoons (2014-2015), wave, current, river discharge, suspended sediment concentration, and bathymetry were measured. These data were collected in shallow sub-tidal water, on the muddy flat and within the mangrove forest. The observed data show that waves and current are dissipated quickly from shallow coastal water into the mangroves. They also depend on topography changes, characteristic in mangroves. Suspended sediment concentration increases from shallow water into the mangroves, and fluctuates according to tides, waves and currents. The analyzed data in Cu Lao Dung and Rach Goc are compared and from that, the main factors causing the erosion and deposition in these studied sites are explained.

The biological activity of botulinum neurotoxin type C is dependent upon novel types of ganglioside binding sites.

PubMed

Strotmeier, Jasmin; Gu, Shenyan; Jutzi, Stephan; Mahrhold, Stefan; Zhou, Jie; Pich, Andreas; Eichner, Timo; Bigalke, Hans; Rummel, Andreas; Jin, Rongsheng; Binz, Thomas

2011-07-01

The seven botulinum neurotoxins (BoNT) cause muscle paralysis by selectively cleaving core components of the vesicular fusion machinery. Their extraordinary activity primarily relies on highly specific entry into neurons. Data on BoNT/A, B, E, F and G suggest that entry follows a dual receptor interaction with complex gangliosides via an established ganglioside binding region and a synaptic vesicle protein. Here, we report high resolution crystal structures of the BoNT/C cell binding fragment alone and in complex with sialic acid. The WY-motif characteristic of the established ganglioside binding region was located on an exposed loop. Sialic acid was co-ordinated at a novel position neighbouring the binding pocket for synaptotagmin in BoNT/B and G and the sialic acid binding site in BoNT/D and TeNT respectively. Employing synaptosomes and immobilized gangliosides binding studies with BoNT/C mutants showed that the ganglioside binding WY-loop, the newly identified sialic acid-co-ordinating pocket and the area corresponding to the established ganglioside binding region of other BoNTs are involved in ganglioside interaction. Phrenic nerve hemidiaphragm activity tests employing ganglioside deficient mice furthermore evidenced that the biological activity of BoNT/C depends on ganglioside interaction with at least two binding sites. These data suggest a unique cell binding and entry mechanism for BoNT/C among clostridial neurotoxins. © 2011 Blackwell Publishing Ltd.

What is Known About Health and Morbidity in the Pediatric Population of Muslim Bedouins in Southern Israel: A Descriptive Review of the Literature from the Past Two Decades.

PubMed

Treister-Goltzman, Yulia; Peleg, Roni

2015-06-01

The Bedouins comprise one of the ethnic groups in Israeli society. They are Muslims, most of who live in the Negev desert region of southern Israel and live by their unique traditions and customs. At the present they are going through a period of "society in transition", a unique condition that has ramifications for health and morbidity. In recent years the number of publications on the health of Bedouins in the Negev has increased. Recognition of unique socio-economic features, characteristics of health and diseases can help the medical team treat various health problems in this population as well as other populations with similar characteristics. In the present paper we survey and discuss publications on the health of Bedouin children over the past 20 years.

The News about Hispanics.

ERIC Educational Resources Information Center

Russell, Cheryl

1983-01-01

Describes the geographic distribution, demographic characteristics, and consumer preferences of Puerto Ricans, Mexicans, and Cubans in the United States. Suggests that a projected rapid growth in numbers of Hispanics with unique characteristics will produce a consumer market that will require special attention from businesses. (Author/MJL)

Perimeter intrusion detection and assessment system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eaton, M.J.; Jacobs, J.; McGovern, D.E.

1977-11-01

To obtain an effective perimeter intrusion detection system requires careful sensor selection, procurement, and installation. The selection process involves a thorough understanding of the unique site features and how these features affect the performance of each type of sensor. It is necessary to develop procurement specifications to establish acceptable sensor performance limits. Careful explanation and inspection of critical installation dimensions is required during on-site construction. The implementation of these activities at a particular site is discussed.

Habitat segregation of Ambystoma gracile and Ambystoma macrodactylum in mountain ponds, Mount Rainer National Park, Washington, USA

USGS Publications Warehouse

Hoffman, Robert L.; Larson, Gary L.; Brokes, Brendan J.

2003-01-01

Ambystoma gracile (Baird) and Ambystoma macrodactylum Baird are common salamander species occupying key trophic positions in mountain ponds and lakes of Mount Rainier National Park (MORA). Based on amphibian surveys conducted in ponds and lakes in the northeast quadrant of MORA during the summers of 1993a??1995, 20 sites were resampled in 1996 to document and evaluate distributions of the two species relative to site habitat characteristics. Distributions of larvae were assessed either by nearshore snorkel or visual encounter surveys of each study site. Twelve environmental variables representing pond and lake physical characteristics, water quality, and nutrient concentrations were measured. The occurrences at each site of three major habitat components (i.e., pond bottom firmness, coarse woody debris, and emergent/aquatic vegetation) were qualitatively estimated. Allotopic distributions of larval populations of both species were related to site elevation, maximum depth, organic content of bottom substrates, and surface area. Ambystoma gracile sites were larger, deeper, lower in elevation, had flocculent sediment higher in organic content, abundant coarse woody debris, and limited emergent/aquatic vegetation relative to A. macrodactylum sites. Ambystoma macrodactylum sites were smaller, shallower, higher in elevation, had firm sediments low in organic content, and had a greater occurrence of emergent/aquatic vegetation than did A. gracile sites. Two sites supported syntopic populations and exhibited many of the habitat characteristics observed at sites inhabited by each species. The distribution of each species in MORA was related to the interconnection between habitat characteristics of ponds and lakes and species life-history patterns and possibly interspecific interactions.

A new 0.9 Ma oxygen isotope stratigraphy for a shallow-water sedimentary transect across three IODP 317 sites in the Canterbury Bight of southwest Pacific Ocean

NASA Astrophysics Data System (ADS)

Ding, Xuan; Wu, YingYing

2016-04-01

Sedimentary records in shallow-water environment provide unique opportunity to further our understanding on the regional relative sea level changes in relation to global climate change. Here we present a new 0.9 Ma oxygen isotope stratigraphy for a shallow-water sedimentary transect across three IODP 317 sites in the Canterbury Bight of southwest Pacific Ocean. The three sites are located on the eastern margin of the South Island of New Zealand, including a continental slope site, IODP317-U1352 and two continental shelf sites, IODP317-U1354 and IODP317-U1351. We first generated high resolution benthic foraminifers (Nonionella flemingi) δ18O records for the three sites and a planktonic (Globigerina bulloides) record for the U1352B. An initial chronological framework for the benthic δ18O record of the U1352B was constructed using 8 accelerator mass spectrometry (AMS) radiocarbon dates and 4 biostratigraphic events. Then a refined age model was established by correlating the U1352B benthic δ18O record with the EDC δD record on the AICC2012 time-scale, and the LR04 benthic δ18O stack. Although the U1354B and U1351B have lower sedimentation rates, their benthic δ18O records correlate well with that of U1352B. In order to ensure the accuracy of the chronostratigraphic framework established, we also analyzed the characteristics of sedimentary grain size and the planktonic and benthic δ18O values. In accord with the adjacent sites, the results show that the melt of Southern Alps glaciers due to the warming climate during MIS 11 and 5.5 led to the increased fresh water delivery, with massive terrigenous deposit; and the warm SST during the MIS7 is related with the STF migration, which led to strong current activity, with coarser grain size. Meanwhile, records of benthic δ18O, sedimentation rate and content of >63μm coarse fraction of site U1352 all indicate the MIS 20 was indeed a colder interval compared to subsequent glacial times.

Observations of Cold Pool Properties during GoAmazon2014/5

NASA Astrophysics Data System (ADS)

Mayne, S. L.; Schumacher, C.; MacDonald, L.; Turner, D. D.

2017-12-01

Convectively generated cold pools are instrumental in both the development of the sub-cloud layer and the organization of deep convection. Despite this, analyses of cold pools in the tropics are constrained by a lack of observational data; insight into the phenomena therefore relies heavily on numerical models. GoAmazon2014/5, a 2-year DOE-sponsored field campaign centered on Manacapuru, Brazil in the central Amazon, provides a unique opportunity to characterize tropical cold pools and allows for the comparison of observational data with theoretical results from model cold pool simulations and parameterizations. This investigation analyzes radar, disdrometer, and profiler measurements at the DOE mobile facility site to study tropical cold pool characteristics. The Brazilian military (SIPAM) operational S-band radar in Manaus is used to provide a broad context of convective systems, while measurements from Parsivel disdrometers are used to assess drop-size distributions (DSDs) at the surface. A unique aspect of this research is the use of the Atmospheric Emitted Radiance Interferometer (AERI) instrument, which utilizes down-welling IR measurements to obtain vertical profiles of thermodynamic quantities such as temperature and water vapor in the lowest few km of the atmosphere. Combined with surface observations and sounding data, these datasets will result in a thorough investigation of the horizontal and vertical characteristics of cold pools over the tropical rain forest. Preliminary analyses of 20 events reveal a mean cold pool height of 220 m and a mean radius of approximately 8.5 km. The average cold pool experienced a temperature (specific humidity) decrease of approximately 1 K (0.4 g/kg) at the surface. The temperature decrease is consistent with modeling studies and limited observations from previous studies over the tropics. The small decrease in specific humidity is attributed to the high moisture content within the cold pools. AERI retrievals of potential temperature and specific humidity profiles show promising similarities with theoretical results produced using the cold pool parameterization presented by Del Genio et al. (2015); however, results are sensitive to both the mass of air injected into the cold pool after its formation, and the thermodynamic characteristics of the downdraft.

Combining Comprehensive Analysis of Off-Site Lambda Phage Integration with a CRISPR-Based Means of Characterizing Downstream Physiology.

PubMed

Tanouchi, Yu; Covert, Markus W

2017-09-19

During its lysogenic life cycle, the phage genome is integrated into the host chromosome by site-specific recombination. In this report, we analyze lambda phage integration into noncanonical sites using next-generation sequencing and show that it generates significant genetic diversity by targeting over 300 unique sites in the host Escherichia coli genome. Moreover, these integration events can have important phenotypic consequences for the host, including changes in cell motility and increased antibiotic resistance. Importantly, the new technologies that we developed to enable this study-sequencing secondary sites using next-generation sequencing and then selecting relevant lysogens using clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9-based selection-are broadly applicable to other phage-bacterium systems. IMPORTANCE Bacteriophages play an important role in bacterial evolution through lysogeny, where the phage genome is integrated into the host chromosome. While phage integration generally occurs at a specific site in the host chromosome, it is also known to occur at other, so-called secondary sites. In this study, we developed a new experimental technology to comprehensively study secondary integration sites and discovered that phage can integrate into over 300 unique sites in the host genome, resulting in significant genetic diversity in bacteria. We further developed an assay to examine the phenotypic consequence of such diverse integration events and found that phage integration can cause changes in evolutionarily relevant traits such as bacterial motility and increases in antibiotic resistance. Importantly, our method is readily applicable to other phage-bacterium systems. Copyright © 2017 Tanouchi and Covert.

Unique Characteristics of High-Cost Users of Medical Care With Comorbid Mental Illness or Addiction in a Population-Based Cohort.

PubMed

Hensel, Jennifer M; Taylor, Valerie H; Fung, Kinwah; de Oliveira, Claire; Vigod, Simone N

To understand whether high-cost users of medical care with and without comorbid mental illness or addiction differ in terms of their sociodemographic and health characteristics. Unique characteristics would warrant different considerations for interventions and service design aimed at reducing unnecessary health care utilization and associated costs. From the top 10% of Ontarians ranked by total medical care costs during fiscal year 2011/2012 (N = 314,936), prior 2-year mental illness or addiction diagnoses were determined from administrative data. Sociodemographics, medical illness characteristics, medical costs, and utilization were compared between those high-cost users of medical care with and without comorbid mental illness or addiction. Odds of being a frequent user of inpatient (≥3 admissions) and emergency (≥5 visits) services were compared between groups, adjusting for age, sex, socioeconomic status and medical illness characteristics. High-cost users of medical care with comorbid mental illness or addiction were younger, had a lower socioeconomic status, had greater historical medical morbidity, and had higher total medical care costs (mean excess of $2,031/user) than those without. They were more likely to be frequent users of inpatient (12.8% vs 10.2%; adjusted OR, 1.14; 95% CI: 1.12-1.17) and emergency (8.4% vs 4.8%; adjusted OR, 1.55; 95% CI: 1.50-1.59) services. Effect sizes were larger in major mood, psychotic, and substance use disorder subgroups. High-cost medical care users with mental illness or addiction have unique characteristics with respect to sociodemographics and service utilization patterns to consider in interventions and policies for this patient group. Copyright © 2018 Academy of Consultation-Liaison Psychiatry. Published by Elsevier Inc. All rights reserved.

Identification of residues that control Li+ versus Na+ dependent Ca2+ exchange at the transport site of the mitochondrial NCLX.

PubMed

Roy, Soumitra; Dey, Kuntal; Hershfinkel, Michal; Ohana, Ehud; Sekler, Israel

2017-06-01

The Na + /Ca 2+ /Li + exchanger (NCLX) is a member of the Na + /Ca 2+ exchanger family. NCLX is unique in its capacity to transport both Na + and Li + , unlike other members, which are Na + selective. The major aim of this study was twofold, i.e., to identify NCLX residues that confer Li + or Na + selective Ca 2+ transport and map their putative location on NCLX cation transport site. We combined molecular modeling to map transport site of NCLX with euryarchaeal H + /Ca 2+ exchanger, CAX_Af, and fluorescence analysis to monitor Li + versus Na + dependent mitochondrial Ca 2+ efflux of transport site mutants of NCLX in permeabilized cells. Mutation of Asn149, Pro152, Asp153, Gly176, Asn467, Ser468, Gly494 and Asn498 partially or strongly abolished mitochondrial Ca 2+ exchange activity in intact cells. In permeabilized cells, N149A, P152A, D153A, N467Q, S468T and G494S demonstrated normal Li + /Ca 2+ exchange activity but a reduced Na + /Ca 2+ exchange activity. On the other hand, D471A showed dramatically reduced Li + /Ca 2+ exchange, but Na + /Ca 2+ exchange activity was unaffected. Finally, simultaneous mutation of four putative Ca 2+ binding residues was required to completely abolish both Na + /Ca 2+ and Li + /Ca 2+ exchange activities. We identified distinct Na + and Li + selective residues in the NCLX transport site. We propose that functional segregation in Li + and Na + sites reflects the functional properties of NCLX required for Ca 2+ exchange under the unique membrane potential and ion gradient across the inner mitochondrial membrane. The results of this study provide functional insights into the unique Li + and Na + selectivity of the mitochondrial exchanger. This article is part of a Special Issue entitled: ECS Meeting edited by Claus Heizmann, Joachim Krebs and Jacques Haiech. Copyright © 2017 Elsevier B.V. All rights reserved.

Surface-water-quality assessment of the Yakima River Basin in Washington: Overview of major findings, 1987-91

USGS Publications Warehouse

Morace, Jennifer L.; Fuhrer, Gregory J.; Rinella, Joseph F.; McKenzie, Stuart W.; Gannett, Marshall W.; Bramblett, Karen L.; Pogue, Ted R.; Skach, Kenneth A.; Embrey, Sandra S.; Cuffney, Thomas F.; Meador, Michael R.; Porter, Stephen D.; Gurtz, Martin E.

1999-01-01

An ecological assessment of the Yakima River Basin ranked physical, chemical, and biological conditions at impaired (degraded) sites against reference sites in an effort to understand how land use changes physical and chemical site characteristics and how biota respond to these changes. For this assessment, the basin was divided into four natural ecological categories: (1) Cascades ecoregion, (2) Eastern Cascades Slopes and Foothills ecoregion, (3) Columbia Basin ecoregion, and (4) large rivers. Each of these categories has a unique combination of climate and landscape features that produces a distinctive terrestrial vegetation assemblage. In the combined Cascades and Eastern Cascades site group, which had the fewest impaired sites, the metals index was the only physical and chemical index that indicated any impairment. The moderate levels of impairment noted in the invertebrate and algal communities were not, however, associated with metals, and may have been related to the effects of logging, although the intensity of logging was not directly quantified in this study. Sites in the Columbia Basin site group were all moderately or severely impaired with the exception of the two reference sites (Umtanum Creek and Satus Creek below Dry Creek), which showed no physical, chemical, or biological impairment. Three sites were heavily affected by agriculture (Granger Drain, Moxee Drain, and Spring Creek) and were listed as severely impaired by most of the physical, chemical, and biological condition indices. Agriculture was the primary cause of the impairment of biological communities in this site group. The primary physical and chemical indicators of agricultural effects were nutrients, pesticides, dissolved solids, and substrate embeddedness, which all tended to increase with agricultural intensity. The biological effects of agriculture were manifested by a decrease in the abundance and number of native species of fish and invertebrates, a shift in algal communities to species indicative of eutrophic conditions, and higher abundances. There was also an increase in the abundance and number of nonnative fish species due to the prevalence of fish that are largely tolerant of nutrient-rich conditions. Main stem (large river) sites downstream from the city of Yakima exhibited severe impairment of fish communities associated with high levels of pesticides in fish tissues and the presence of external anomalies on fish.

Maternal education and intelligence predict offspring diet and nutritional status.

PubMed

Wachs, Theodore D; Creed-Kanashiro, Hilary; Cueto, Santiago; Jacoby, Enrique

2005-09-01

The traditional assumption that children's nutritional deficiencies are essentially due either to overall food scarcity or to a lack of family resources to purchase available food has been increasingly questioned. Parental characteristics represent 1 type of noneconomic factor that may be related to variability in children's diets and nutritional status. We report evidence on the relation of 2 parental characteristics, maternal education level and maternal intelligence, to infant and toddler diet and nutritional status. Our sample consisted of 241 low-income Peruvian mothers and their infants assessed from 3 to 12 mo, with a further follow-up of 104 of these infants at 18 mo of age. Using a nonexperimental design, we related measures of level of maternal education, maternal intelligence, and family socioeconomic status to infant anthropometry, duration of exclusive breast-feeding, adequacy of dietary intake, and iron status. Results indicated unique positive relations between maternal education level and the extent of exclusive breast-feeding. Significant relations between maternal education and offspring length were partially mediated by maternal height. There also were unique positive relations between maternal intelligence and quality of offspring diet and hemoglobin level. All findings remained significant even after controlling for family socioeconomic characteristics. This pattern of results illustrates the importance of parental characteristics in structuring the adequacy of offspring diet. Maternal education and intelligence appear to have unique influences upon different aspects of the diet and nutritional status of offspring.

Well-posedness of characteristic symmetric hyperbolic systems

NASA Astrophysics Data System (ADS)

Secchi, Paolo

1996-06-01

We consider the initial-boundary-value problem for quasi-linear symmetric hyperbolic systems with characteristic boundary of constant multiplicity. We show the well-posedness in Hadamard's sense (i.e., existence, uniqueness and continuous dependence of solutions on the data) of regular solutions in suitable functions spaces which take into account the loss of regularity in the normal direction to the characteristic boundary.

A Digital Hydrologic Network Supporting NAWQA MRB SPARROW Modeling--MRB_E2RF1WS

USGS Publications Warehouse

Brakebill, J.W.; Terziotti, S.E.

2011-01-01

A digital hydrologic network was developed to support SPAtially Referenced Regression on Watershed attributes (SPARROW) models within selected regions of the United States. These regions correspond with the U.S. Geological Survey's National Water Quality Assessment (NAWQA) Program Major River Basin (MRB) study units 2, 3, 4, 5, and 7 (Preston and others, 2009). MRB2, covers the South Atlantic-Gulf and Tennessee River basins. MRB3, covers the Great Lakes, Ohio, Upper Mississippi, and Souris-Red-Rainy River basins. MRB4, covers the Missouri River basins. MRB5, covers the Lower Mississippi, Arkansas-White-Red, and Texas-Gulf River basins. MRB7, covers the Pacific Northwest River basins. The digital hydrologic network described here represents surface-water pathways (MRB_E2RF1) and associated catchments (MRB_E2RF1WS). It serves as the fundamental framework to spatially reference and summarize explanatory information supporting nutrient SPARROW models (Brakebill and others, 2011; Wieczorek and LaMotte, 2011). The principal geospatial dataset used to support this regional effort was based on an enhanced version of a 1:500,000 scale digital stream-reach network (ERF1_2) (Nolan et al., 2002). Enhancements included associating over 3,500 water-quality monitoring sites to the reach network, improving physical locations of stream reaches at or near monitoring locations, and generating drainage catchments based on 100m elevation data. A unique number (MRB_ID) identifies each reach as a single unit. This unique number is also shared by the catchment area drained by the reach, thus spatially linking the hydrologically connected streams and the respective drainage area characteristics. In addition, other relevant physical, environmental, and monitoring information can be associated to the common network and accessed using the unique identification number.

A Digital Hydrologic Network Supporting NAWQA MRB SPARROW Modeling--MRB_E2RF1

USGS Publications Warehouse

Brakebill, J.W.; Terziotti, S.E.

2011-01-01

A digital hydrologic network was developed to support SPAtially Referenced Regression on Watershed attributes (SPARROW) models within selected regions of the United States. These regions correspond with the U.S. Geological Survey's National Water Quality Assessment (NAWQA) Program Major River Basin (MRB) study units 2, 3, 4, 5, and 7 (Preston and others, 2009). MRB2, covers the South Atlantic-Gulf and Tennessee River basins. MRB3, covers the Great Lakes, Ohio, Upper Mississippi, and Souris-Red-Rainy River basins. MRB4, covers the Missouri River basins. MRB5, covers the Lower Mississippi, Arkansas-White-Red, and Texas-Gulf River basins. MRB7, covers the Pacific Northwest River basins. The digital hydrologic network described here represents surface-water pathways (MRB_E2RF1) and associated catchments (MRB_E2RF1WS). It serves as the fundamental framework to spatially reference and summarize explanatory information supporting nutrient SPARROW models (Brakebill and others, 2011; Wieczorek and LaMotte, 2011). The principal geospatial dataset used to support this regional effort was based on an enhanced version of a 1:500,000 scale digital stream-reach network (ERF1_2) (Nolan et al., 2002). Enhancements included associating over 3,500 water-quality monitoring sites to the reach network, improving physical locations of stream reaches at or near monitoring locations, and generating drainage catchments based on 100m elevation data. A unique number (MRB_ID) identifies each reach as a single unit. This unique number is also shared by the catchment area drained by the reach, thus spatially linking the hydrologically connected streams and the respective drainage area characteristics. In addition, other relevant physical, environmental, and monitoring information can be associated to the common network and accessed using the unique identification number.

The Unique Morgue Ubiquitination Protein Is Conserved in a Diverse but Restricted Set of Invertebrates

PubMed Central

Zhou, Ying; Carpenter, Zachary W.; Brennan, Gregory

2009-01-01

Drosophila Morgue is a unique ubiquitination protein that facilitates programmed cell death and associates with DIAP1, a critical cell death inhibitor with E3 ubiquitin ligase activity. Morgue possesses a unique combination of functional domains typically associated with distinct types of ubiquitination enzymes. This includes an F box characteristic of the substrate-binding subunit in Skp, Cullin, and F box (SCF)-type ubiquitin E3 ligase complexes and a variant ubiquitin E2 conjugase domain where the active site cysteine is replaced by a glycine. Morgue also contains a single C4-type zinc finger motif. This architecture suggests potentially novel ubiquitination activities for Morgue. In this study, we address the evolutionary origins of this distinctive protein utilizing a combination of bioinformatics and molecular biology approaches. We find that Morgue exhibits widespread but restricted phylogenetic distribution among metazoans. Morgue proteins were identified in a wide range of Protostome phyla, including Arthropoda, Annelida, Mollusca, Nematoda, and Platyhelminthes. However, with one potential exception, Morgue was not detected in Deuterostomes, including Chordates, Hemichordates, or Echinoderms. Morgue was also not found in Ctenophora, Cnidaria, Placozoa, or Porifera. Characterization of Morgue sequences within specific animal lineages suggests that gene deletion or acquisition has occurred during divergence of nematodes and that at least one arachnid expresses an atypical form of Morgue consisting only of the variant E2 conjugase domain. Analysis of the organization of several morgue genes suggests that exon-shuffling events have contributed to the evolution of the Morgue protein. These results suggest that Morgue mediates conserved and distinctive ubiquitination functions in specific cell death pathways. PMID:19602541

Evolution of Wholesale Electricity Market Design with Increasing Levels of Renewable Generation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ela, E.; Milligan, M.; Bloom, A.

2014-09-01

Variable generation such as wind and photovoltaic solar power has increased substantially in recent years. Variable generation has unique characteristics compared to the traditional technologies that supply energy in the wholesale electricity markets. These characteristics create unique challenges in planning and operating the power system, and they can also influence the performance and outcomes from electricity markets. This report focuses on two particular issues related to market design: revenue sufficiency for long-term reliability and incentivizing flexibility in short-term operations. The report provides an overview of current design and some designs that have been proposed by industry or researchers.

Improved Design of Optical MEMS Using the SUMMiT Fabrication Process

DOE Office of Scientific and Technical Information (OSTI.GOV)

Michalicek, M.A.; Comtois, J.H.; Barron, C.C.

This paper describes the design and fabrication of optical Microelectromechanical Systems (MEMS) devices using the Sandia Ultra planar Multilevel MEMS Technology (SUMMiT) fabrication process. This state of the art process, offered by Sandia National Laboratories, provides unique and very advantageous features which make it ideal for optical devices. This enabling process permits the development of micromirror devices with near ideal characteristics which have previously been unrealizable in standard polysilicon processes. This paper describes such characteristics as elevated address electrodes, individual address wiring beneath the device, planarized mirror surfaces, unique post-process metallization, and the best active surface area to date.

The China Patient-Centered Evaluative Assessment of Cardiac Events (China PEACE) retrospective study of acute myocardial infarction: study design.

PubMed

Dharmarajan, Kumar; Li, Jing; Li, Xi; Lin, Zhenqiu; Krumholz, Harlan M; Jiang, Lixin

2013-11-01

Cardiovascular diseases are rising as a cause of death and disability in China. To improve outcomes for patients with these conditions, the Chinese government, academic researchers, clinicians, and >200 hospitals have created China Patient-Centered Evaluative Assessment of Cardiac Events (China PEACE), a national network for research and performance improvement. The first study from China PEACE, the Retrospective Study of Acute Myocardial Infarction (China PEACE-Retrospective AMI Study), is designed to promote improvements in acute myocardial infarction (AMI) quality of care by generating knowledge about the characteristics, treatments, and outcomes of patients hospitalized with AMI across a representative sample of Chinese hospitals during the past decade. The China PEACE-Retrospective AMI Study will examine >18 000 patient records from 162 hospitals identified using a 2-stage cluster sampling design within economic-geographic regions. Records were chosen from 2001, 2006, and 2011 to identify temporal trends. Data quality will be monitored by a central coordinating center and will, in particular, address case ascertainment, data abstraction, and data management. Analyses will examine patient characteristics, diagnostic testing patterns, in-hospital treatments, in-hospital outcomes, and variation in results by time and site of care. In addition to publications, data will be shared with participating hospitals and the Chinese government to develop strategies to promote quality improvement. The China PEACE-Retrospective AMI Study is the first to leverage the China PEACE platform to better understand AMI across representative sites of care and during the past decade in China. The China PEACE collaboration among government, academicians, clinicians, and hospitals is poised to translate research about trends and patterns of AMI practices and outcomes into improved care for patients. URL: http://www.clinicaltrials.gov. Unique identifier: NCT01624883.

An Event-Level Analysis of Condomless Anal Intercourse with a HIV-Discordant or HIV Status-Unknown Partner Among Black Men Who Have Sex with Men from a Multi-site Study.

PubMed

Yang, Cui; Latkin, Carl; Tobin, Karin; Seal, David; Koblin, Beryl; Chander, Geetanjali; Siconolfi, Daniel; Flores, Stephen; Spikes, Pilgrim

2018-05-19

Despite the high HIV incidence and prevalence among black men who have sex with men (BMSM), little research has examined partner characteristics, partner seeking venue, sexual position, substance use, and sexual risk behavior at the sex event-level among BMSM. Using the baseline data from a multi-site study of 807 BMSM stratified by their HIV status, the goal of this study was to conduct a detailed event-level analysis of 1577 male anal sex events to assess the factors associated with condomless anal intercourse (CLAI) with a HIV-discordant or HIV status-unknown partner. We found CLAI with an HIV-discordant or unknown HIV status partner among HIV-negative BMSM was negatively associated with having sex with a main partner, and was positively associated with taking both receptive and insertive sexual positions during sex. As compared to a sex partner met at bar, night club or dance club, HIV-positive BMSM were less likely to engage in CLAI with HIV-discordant and unknown HIV status partner met at party or friend's house or at community organizations. HIV-positive BMSM had lower odds of engaging in CLAI with HIV-discordant and unknown HIV status partner if they had insertive sexual position or both receptive and insertive sexual positions. These results underscore the importance of delineating unique sex event-level factors associated with sexual risk behavior depending on individuals' HIV status. Our findings suggest event-level partner characteristics, sexual position, and partner seeking venues may contribute to disparities in HIV incidence.

Discovering transcription factor binding sites in highly repetitive regions of genomes with multi-read analysis of ChIP-Seq data.

PubMed

Chung, Dongjun; Kuan, Pei Fen; Li, Bo; Sanalkumar, Rajendran; Liang, Kun; Bresnick, Emery H; Dewey, Colin; Keleş, Sündüz

2011-07-01

Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) is rapidly replacing chromatin immunoprecipitation combined with genome-wide tiling array analysis (ChIP-chip) as the preferred approach for mapping transcription-factor binding sites and chromatin modifications. The state of the art for analyzing ChIP-seq data relies on using only reads that map uniquely to a relevant reference genome (uni-reads). This can lead to the omission of up to 30% of alignable reads. We describe a general approach for utilizing reads that map to multiple locations on the reference genome (multi-reads). Our approach is based on allocating multi-reads as fractional counts using a weighted alignment scheme. Using human STAT1 and mouse GATA1 ChIP-seq datasets, we illustrate that incorporation of multi-reads significantly increases sequencing depths, leads to detection of novel peaks that are not otherwise identifiable with uni-reads, and improves detection of peaks in mappable regions. We investigate various genome-wide characteristics of peaks detected only by utilization of multi-reads via computational experiments. Overall, peaks from multi-read analysis have similar characteristics to peaks that are identified by uni-reads except that the majority of them reside in segmental duplications. We further validate a number of GATA1 multi-read only peaks by independent quantitative real-time ChIP analysis and identify novel target genes of GATA1. These computational and experimental results establish that multi-reads can be of critical importance for studying transcription factor binding in highly repetitive regions of genomes with ChIP-seq experiments.

Ligand Displacement Reaction Paths in a Diiron Hydrogenase Active Site Model Complex.

PubMed

Blank, Jan H; Moncho, Salvador; Lunsford, Allen M; Brothers, Edward N; Darensbourg, Marcetta Y; Bengali, Ashfaq A

2016-08-26

The mechanism and energetics of CO, 1-hexene, and 1-hexyne substitution from the complexes (SBenz)2 [Fe2 (CO)6 ] (SBenz=SCH2 Ph) (1-CO), (SBenz)2 [Fe2 (CO)5 (η(2) -1-hexene)] (1-(η(2) -1-hexene)), and (SBenz)2 [Fe2 (CO)5 (η(2) -1-hexyne)] (1-(η(2) -1-hexyne)) were studied by using time-resolved infrared spectroscopy. Exchange of both CO and 1-hexyne by P(OEt)3 and pyridine, respectively, proceeds by a bimolecular mechanism. As similar activation enthalpies are obtained for both reactions, the rate-determining step in both cases is assumed to be the rotation of the Fe(CO)2 L (L=CO or 1-hexyne) unit to accommodate the incoming ligand. The kinetic profile for the displacement of 1-hexene is quite different than that for the alkyne and, in this case, both reaction channels, that is, dissociative (SN 1) and associative (SN 2), were found to be competitive. Because DFT calculations predict similar binding enthalpies of alkene and alkyne to the iron center, the results indicate that the bimolecular pathway in the case of the alkyne is lower in free energy than that of the alkene. In complexes of this type, subtle changes in the departing ligand characteristics and the nature of the mercapto bridge can influence the exchange mechanism, such that more than one reaction pathway is available for ligand substitution. The difference between this and the analogous study of (μ-pdt)[Fe(CO)3 ]2 (pdt=S(CH2 )3 S) underscores the unique characteristics of a three-atom S-S linker in the active site of diiron hydrogenases. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

A modeling study for structure features of β-N-acetyl-D-hexosaminidase from Ostrinia furnacalis and its novel inhibitor allosamidin: species selectivity and multi-target characteristics.

PubMed

Wang, Yanli; Liu, Tian; Yang, Qing; Li, Zhong; Qian, Xuhong

2012-04-01

Insect β-N-acetyl-D-hexosaminidase, a chitin degrading enzyme, is physiologically important during the unique life cycle of the insect. OfHex1, a β-N-acetyl-D-hexosaminidase from the insect, Ostrinia furna, which was obtained by our laboratory (Gen Bank No.: ABI81756.1), was studied by molecular modeling as well as by molecular docking with its inhibitor, allosamidin. 3D model of OfHex1 was built through the ligand-supported homology modeling approach. The binding modes of its substrate and inhibitor were proposed through docking and cluster analysis. The pocket's size and shape of OfHex1 differ from that of human β-N-acetyl-D-hexosaminidase, which determined that allosamidin can selectively inhibit OfHex1 instead of human β-N-acetyl-D-hexosaminidase. Moreover, the multi-target characteristics of allosamidin that inhibit enzymes from different families, OfHex1 (EC 3.2.1.52; GH20) and chitinase (EC 3.2.1.14; GH18), were compared. The common -1/+1 sugar-binding site of chitinase and OfHex1, and the -2/-3 sugar-binding site in chitinase contribute to the binding of allosamidin. This work, at molecular level, proved that OfHex1 could be a potential species-specific target for novel green pesticide design and also provide the possibility to develop allosamidin or its derivatives as a new type of insecticide to 'hit two birds with one stone', which maybe become a novel strategy in pest control. © 2011 John Wiley & Sons A/S.

Distinguishing tracheal and esophageal tissues with hyperspectral imaging and fiber-optic sensing

NASA Astrophysics Data System (ADS)

Nawn, Corinne D.; Souhan, Brian E.; Carter, Robert, III; Kneapler, Caitlin; Fell, Nicholas; Ye, Jing Yong

2016-11-01

During emergency medical situations, where the patient has an obstructed airway or necessitates respiratory support, endotracheal intubation (ETI) is the medical technique of placing a tube into the trachea in order to facilitate adequate ventilation of the lungs. Complications during ETI, such as repeated attempts, failed intubation, or accidental intubation of the esophagus, can lead to severe consequences or ultimately death. Consequently, a need exists for a feedback mechanism to aid providers in performing successful ETI. Our study examined the spectral reflectance properties of the tracheal and esophageal tissue to determine whether a unique spectral profile exists for either tissue for the purpose of detection. The study began by using a hyperspectral camera to image excised pig tissue samples exposed to white and UV light in order to capture the spectral reflectance properties with high fidelity. After identifying a unique spectral characteristic of the trachea that significantly differed from esophageal tissue, a follow-up investigation used a fiber optic probe to confirm the detectability and consistency of the different reflectance characteristics in a pig model. Our results characterize the unique and consistent spectral reflectance characteristic of tracheal tissue, thereby providing foundational support for exploiting spectral properties to detect the trachea during medical procedures.

Single Subject Research: Applications to Special Education

ERIC Educational Resources Information Center

Cakiroglu, Orhan

2012-01-01

Single subject research is a scientific research methodology that is increasingly used in the field of special education. Therefore, understanding the unique characteristics of single subject research methodology is critical both for educators and practitioners. Certain characteristics make single subject research one of the most preferred…

Combustion characteristics of crude jatropha oil droplets using rhodium liquid as a homogeneous combustion catalyst

NASA Astrophysics Data System (ADS)

Nanlohy, Hendry Y.; Wardana, I. N. G.; Hamidi, N.; Yuliati, L.

2018-01-01

Combustion characteristics of crude jatropha oil droplet at room temperature with and without catalyst have been studied experimentally. Its combustion characteristics have been observed by igniting the oil droplet on a junction of a thermocouple, and the combustion characteristics of oil droplets are observed using a high-speed camera. The results show that the uniqueness of crude jatropha oil as alternative fuel is evidenced by the different stages of combustion caused by thermal cracking in burning droplets. The results also show that the role of the catalyst is not only an accelerator agent, but there are other unique functions and roles as a stabilizer. Moreover, the results also found that the catalyst was able to shorten the ignition timing and burnout time. This phenomenon proves that the presence of catalysts alters and weakens the structure of the triglyceride geometry so that the viscosity and flash point is reduced, the fuel absorbs heat well and flammable.

Section 10: Ground Water - Waste Characteristics & Targets

EPA Pesticide Factsheets

HRS Training. The waste characteristics factor category in the ground water pathway is made up of two components: the toxicity/mobility of the most hazardous substance associated with the site and the hazardous waste quantity at the site.

The Uniqueness of EFL Teachers: Perceptions of Japanese Learners

ERIC Educational Resources Information Center

Lee, Joseph J.

2010-01-01

Building on the work of Borg (2006), this article reports on a study of Japanese English as a foreign language (EFL) learners' perceptions of some of the unique characteristics of EFL teachers that distinguish them from teachers of other subjects. The data were collected by means of a questionnaire to which 163 college-level EFL students in Japan…

Using Learning-Strategies Instruction with Students Who Are Gifted and Learning Disabled

ERIC Educational Resources Information Center

Bisland, Amy

2004-01-01

Regardless of prevalence, students who are gifted and learning disabled do exist in America's public schools, and they have unique needs that must be met through our education system (Bees, 1998). Special education teachers, regular education teachers, and teachers of the gifted should be aware of the unique characteristics of students who are…

Examining Shared and Unique Aspects of Social Anxiety Disorder and Autism Spectrum Disorder Using Factor Analysis

ERIC Educational Resources Information Center

White, Susan W.; Bray, Bethany C.; Ollendick, Thomas H.

2012-01-01

Social Anxiety Disorder (SAD) and Autism Spectrum Disorders (ASD) are fairly common psychiatric conditions that impair the functioning of otherwise healthy young adults. Given that the two conditions frequently co-occur, measurement of the characteristics unique to each condition is critical. This study evaluated the structure and construct…

Special Diabetes Program for Indians: Retention in Cardiovascular Risk Reduction

PubMed Central

Manson, Spero M.; Jiang, Luohua; Zhang, Lijing; Beals, Janette; Acton, Kelly J.; Roubideaux, Yvette

2011-01-01

Purpose: This study examined the associations between participant and site characteristics and retention in a multisite cardiovascular disease risk reduction project. Design and Methods: Data were derived from the Special Diabetes Program for Indians Healthy Heart Demonstration Project, an intervention to reduce cardiovascular risk among American Indians and Alaska Natives with diabetes. In 2006, a total of 1,072 participants from 30 participating sites completed baseline questionnaires measuring demographics and sociobehavioral factors. They also underwent a medical examination at baseline and were reassessed annually after baseline. A Provider Annual Questionnaire was administered to staff members of each grantee site at the end of each year to assess site characteristics. Generalized estimating equation models were used to evaluate the relationships between participant and site characteristics and retention 1 year after baseline. Results: Among enrolled participants, 792 (74%) completed their first annual assessment. Participants who completed the first annual assessment tended to be older and had, at baseline, higher body mass index and higher level of physical activity. Site characteristics associated with retention included average age of staff, proportion of female staff members, and percentage of staff members having completed graduate or professional school. Implications: Understanding successful retention must reach beyond individual characteristics of participants to include features of the settings that house the interventions. PMID:21565816

Improving Web Searches: Case Study of Quit-Smoking Web Sites for Teenagers

PubMed Central

Skinner, Harvey

2003-01-01

Background The Web has become an important and influential source of health information. With the vast number of Web sites on the Internet, users often resort to popular search sites when searching for information. However, little is known about the characteristics of Web sites returned by simple Web searches for information about smoking cessation for teenagers. Objective To determine the characteristics of Web sites retrieved by search engines about smoking cessation for teenagers and how information quality correlates with the search ranking. Methods The top 30 sites returned by 4 popular search sites in response to the search terms "teen quit smoking" were examined. The information relevance and quality characteristics of these sites were evaluated by 2 raters. Objective site characteristics were obtained using a page-analysis Web site. Results Only 14 of the 30 Web sites are of direct relevance to smoking cessation for teenagers. The readability of about two-thirds of the 14 sites is below an eighth-grade school level and they ranked significantly higher (Kendall rank correlation, tau = -0.39, P= .05) in search-site results than sites with readability above or equal to that grade level. Sites that ranked higher were significantly associated with the presence of e-mail address for contact (tau = -0.46, P= .01), annotated hyperlinks to external sites (tau = -0.39, P= .04), and the presence of meta description tag (tau = -0.48, P= .002). The median link density (number of external sites that have a link to that site) of the Web pages was 6 and the maximum was 735. A higher link density was significantly associated with a higher rank (tau = -0.58, P= .02). Conclusions Using simple search terms on popular search sites to look for information on smoking cessation for teenagers resulted in less than half of the sites being of direct relevance. To improve search efficiency, users could supplement results obtained from simple Web searches with human-maintained Web directories and learn to refine their searches with more advanced search syntax. PMID:14713656

Lipid binding by the Unique and SH3 domains of c-Src suggests a new regulatory mechanism

PubMed Central

Pérez, Yolanda; Maffei, Mariano; Igea, Ana; Amata, Irene; Gairí, Margarida; Nebreda, Angel R.; Bernadó, Pau; Pons, Miquel

2013-01-01

c-Src is a non-receptor tyrosine kinase involved in numerous signal transduction pathways. The kinase, SH3 and SH2 domains of c-Src are attached to the membrane-anchoring SH4 domain through the flexible Unique domain. Here we show intra- and intermolecular interactions involving the Unique and SH3 domains suggesting the presence of a previously unrecognized additional regulation layer in c-Src. We have characterized lipid binding by the Unique and SH3 domains, their intramolecular interaction and its allosteric modulation by a SH3-binding peptide or by Calcium-loaded calmodulin binding to the Unique domain. We also show reduced lipid binding following phosphorylation at conserved sites of the Unique domain. Finally, we show that injection of full-length c-Src with mutations that abolish lipid binding by the Unique domain causes a strong in vivo phenotype distinct from that of wild-type c-Src in a Xenopus oocyte model system, confirming the functional role of the Unique domain in c-Src regulation. PMID:23416516

How spatio-temporal habitat connectivity affects amphibian genetic structure

PubMed Central

Watts, Alexander G.; Schlichting, Peter E.; Billerman, Shawn M.; Jesmer, Brett R.; Micheletti, Steven; Fortin, Marie-Josée; Funk, W. Chris; Hapeman, Paul; Muths, Erin; Murphy, Melanie A.

2015-01-01

Heterogeneous landscapes and fluctuating environmental conditions can affect species dispersal, population genetics, and genetic structure, yet understanding how biotic and abiotic factors affect population dynamics in a fluctuating environment is critical for species management. We evaluated how spatio-temporal habitat connectivity influences dispersal and genetic structure in a population of boreal chorus frogs (Pseudacris maculata) using a landscape genetics approach. We developed gravity models to assess the contribution of various factors to the observed genetic distance as a measure of functional connectivity. We selected (a) wetland (within-site) and (b) landscape matrix (between-site) characteristics; and (c) wetland connectivity metrics using a unique methodology. Specifically, we developed three networks that quantify wetland connectivity based on: (i) P. maculata dispersal ability, (ii) temporal variation in wetland quality, and (iii) contribution of wetland stepping-stones to frog dispersal. We examined 18 wetlands in Colorado, and quantified 12 microsatellite loci from 322 individual frogs. We found that genetic connectivity was related to topographic complexity, within- and between-wetland differences in moisture, and wetland functional connectivity as contributed by stepping-stone wetlands. Our results highlight the role that dynamic environmental factors have on dispersal-limited species and illustrate how complex asynchronous interactions contribute to the structure of spatially-explicit metapopulations. PMID:26442094

Diagnosis of meningioma by time-resolved fluorescence spectroscopy.

PubMed

Butte, Pramod V; Pikul, Brian K; Hever, Aviv; Yong, William H; Black, Keith L; Marcu, Laura

2005-01-01

We investigate the use of time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) as an adjunctive tool for the intraoperative rapid evaluation of tumor specimens and delineation of tumor from surrounding normal tissue. Tissue autofluorescence is induced with a pulsed nitrogen laser (337 nm, 1.2 ns) and the intensity decay profiles are recorded in the 370 to 500 nm spectral range with a fast digitizer (0.2 ns resolution). Experiments are conducted on excised specimens (meningioma, dura mater, cerebral cortex) from 26 patients (97 sites). Spectral intensities and time-dependent parameters derived from the time-resolved spectra of each site are used for tissue characterization. A linear discriminant analysis algorithm is used for tissue classification. Our results reveal that meningioma is characterized by unique fluorescence characteristics that enable discrimination of tumor from normal tissue with high sensitivity (>89%) and specificity (100%). The accuracy of classification is found to increase (92.8% cases in the training set and 91.8% in the cross-validated set correctly classified) when parameters from both the spectral and the time domain are used for discrimination. Our findings establish the feasibility of using TR-LIFS as a tool for the identification of meningiomas and enables further development of real-time diagnostic tools for analyzing surgical tissue specimens of meningioma or other brain tumors.

Diagnosis of meningioma by time-resolved fluorescence spectroscopy

PubMed Central

Butte, Pramod V.; Pikul, Brian K.; Hever, Aviv; Yong, William H.; Black, Keith L.; Marcu, Laura

2010-01-01

We investigate the use of time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) as an adjunctive tool for the intraoperative rapid evaluation of tumor specimens and delineation of tumor from surrounding normal tissue. Tissue autofluorescence is induced with a pulsed nitrogen laser (337 nm, 1.2 ns) and the intensity decay profiles are recorded in the 370 to 500 nm spectral range with a fast digitizer (0.2 ns resolution). Experiments are conducted on excised specimens (meningioma, dura mater, cerebral cortex) from 26 patients (97 sites). Spectral intensities and time-dependent parameters derived from the time-resolved spectra of each site are used for tissue characterization. A linear discriminant analysis algorithm is used for tissue classification. Our results reveal that meningioma is characterized by unique fluorescence characteristics that enable discrimination of tumor from normal tissue with high sensitivity (>89%) and specificity (100%). The accuracy of classification is found to increase (92.8% cases in the training set and 91.8% in the cross-validated set correctly classified) when parameters from both the spectral and the time domain are used for discrimination. Our findings establish the feasibility of using TR-LIFS as a tool for the identification of meningiomas and enables further development of real-time diagnostic tools for analyzing surgical tissue specimens of meningioma or other brain tumors. PMID:16409091

Time-lapse seismic study of levees in southern New Mexico

USGS Publications Warehouse

Ivanov, J.; Miller, R.D.; Stimac, N.; Ballard, R.F.; Dunbar, J. Joseph; Smullen, S. Steve

2006-01-01

The primary objective of this work was to measure changes in compressional- (Vp) and shear-wave (Vs) velocities in an earthen levee during a ponding experiment designed to simulate flood conditions on the Rio Grande in south New Mexico. Although similar to such experiment, performed an year earlier on the Rio Grande in south Texas, the levee seismic response results are different. This work was similar to previous Preliminary testing at three levee sites, all within a 1 km radius and each with unique physical, EM, and core characteristics, was completed and a single low-conductivity, highly fractured site was selected for investigation. Several different types of seismic data were recorded. Seismic data analysis techniques appraised included P-refraction tomography and Rayleigh surface-wave analysis using multichannel analysis of surface waves (MASW). P-wave velocity change (decrease) was rapid and isolated to one section within the pool confines, which already had anomalously high velocity most likely related to burrowing animals modification of the levee structure. S-wave velocity change was gradual and could be observed along the whole width of the pond within and below the levee. The results within the levee sand core were consistent with the observations of sand S-wave velocity changed due to saturation. ?? 2005 Society of Exploration Geophysicists.

Coastal Wind Profiles In The Mediterranean Area From A Wind Lidar During A Two Year Period

NASA Astrophysics Data System (ADS)

Gullì, Daniel; Avolio, Elenio; Calidonna, Claudia Roberta; Lo Feudo, Teresa; Torcasio, Rosa Claudia; Sempreviva, Anna Maria

2017-04-01

Reliable measurements of vertical profiles of wind speed and direction are the basis for testing models and methodologies of use for wind energy assessment. Modelling coastal areas further introduce the challenge of the coastal discontinuity, which is often not accurately resolved in meso-scale numerical model. Here, we present the analysis of two year of 10-minute averaged wind speed and direction vertical profiles collected during a two-year period from a Wind- lidar ZEPHIR 300® at a coastal suburban area. The lidar is located at the SUPER SITE of CNR-ISAC section of Lamezia Terme, Italy and both dataset and site are unique in the Mediterranean area. The instrument monitors at 10 vertical levels, from 10 m up to 300 m. The analysis is classified according to season, and wind directions for offshore and offshore flow. For onshore flow, we note an atmospheric layer at around 100 m that likely represents the effect an internal boundary layer caused by the sharp coastal discontinuity of the surface characteristics. For offshore flows, the profiles show a layer ranging between 80m and 100m, which might be ascribed to the land night time boundary layer combined to the impact of the building around the mast.

Sequence motifs and prokaryotic expression of the reptilian paramyxovirus fusion protein

USGS Publications Warehouse

Franke, J.; Batts, W.N.; Ahne, W.; Kurath, G.; Winton, J.R.

2006-01-01

Fourteen reptilian paramyxovirus isolates were chosen to represent the known extent of genetic diversity among this novel group of viruses. Selected regions of the fusion (F) gene were sequenced, analyzed and compared. The F gene of all isolates contained conserved motifs homologous to those described for other members of the family Paramyxoviridae including: signal peptide, transmembrane domain, furin cleavage site, fusion peptide, N-linked glycosylation sites, and two heptad repeats, the second of which (HRB-LZ) had the characteristics of a leucine zipper. Selected regions of the fusion gene of isolate Gono-GER85 were inserted into a prokaryotic expression system to generate three recombinant protein fragments of various sizes. The longest recombinant protein was cleaved by furin into two fragments of predicted length. Western blot analysis with virus-neutralizing rabbit-antiserum against this isolate demonstrated that only the longest construct reacted with the antiserum. This construct was unique in containing 30 additional C-terminal amino acids that included most of the HRB-LZ. These results indicate that the F genes of reptilian paramyxoviruses contain highly conserved motifs typical of other members of the family and suggest that the HRB-LZ domain of the reptilian paramyxovirus F protein contains a linear antigenic epitope. ?? Springer-Verlag 2005.

Predictors of adherence among community users of a cognitive behavior therapy website

PubMed Central

Batterham, Philip J; Neil, Alison L; Bennett, Kylie; Griffiths, Kathleen M; Christensen, Helen

2008-01-01

Objective To investigate the predictors of early and late dropout among community users of the MoodGYM website, a five module online intervention for reducing the symptoms of depression. Method Approximately 82,000 users accessed the site in 2006, of which 27% completed one module and 10% completed two or more modules. Adherence was modeled as a trichotomous variable representing non-starters (0 modules), early dropouts (1 module) and late dropouts (2–5 modules). Predictor variables included age, gender, education, location, referral source, depression severity, anxiety severity, dysfunctional thinking, and change in symptom count. Results Better adherence was predicted by higher depression severity, higher anxiety severity, a greater level of dysfunctional thinking, younger age, higher education, being female, and being referred to the site by a mental health professional. In addition, users whose depression severity had improved or remained stable after the first intervention module had higher odds of completing subsequent modules. Conclusions While the effect of age and the null effect of location were in accordance with prior adherence research, the significant effects of gender, education and depression severity were not, and may reflect user characteristics, the content of the intervention and unique aspects of online interventions. Further research directions are suggested to investigate the elements of open access online interventions that facilitate adherence. PMID:19920949

Coverage of the Ebola Virus Disease Epidemic on YouTube.

PubMed

Basch, Corey H; Basch, Charles E; Ruggles, Kelly V; Hammond, Rodney

2015-10-01

The recent Ebola virus disease (EVD) outbreak in 2014-2015 has been the largest and longest lasting to date. Media coverage about the outbreak has been extensive, but there are large gaps in our understanding of the ways in which widely accessed social media sites are used during times of public health crisis. The purpose of this study was to analyze widely viewed videos about EVD on the YouTube video-sharing site. We coded the source, content, and characteristics of the 100 most widely viewed videos about EVD on YouTube. The videos included in the sample were viewed more than 73 million times. The death toll in West Africa was mentioned in nearly one-third of the videos. Over one-third of the videos mentioned how EVD was generally transmitted. There was little mention of treatment and no mention of the need for US funding of disaster preparedness; coordination between local, state, and federal governments; or beds ready for containment. No significant differences in the number of views were identified between video sources with the exception of a significantly higher number of views for "consumer videos" compared with "commercial television videos." With 1 billion unique users a month, YouTube has potential for both enhancing education and spreading misinformation.

The value and potential of multisited ethnography for science education research: a review of Jrène Rahm's Science in the Making at the Margin

NASA Astrophysics Data System (ADS)

Beeman-Cadwallader, Nicole

2012-03-01

Rahm sought to illuminate how children and youth make meaning of science in three out-of-school time programs, and the identity work that is done through the trajectory of their youth toward their young adulthood. Through using multisited ethnography, she asserts that we can learn more about what the youth say about their science learning and science literacy development in these programs. She has revealed through her rich data the potential value of multisited ethnography for science education research. A similar theme of insider/outsider status to science emerged in each of the three sites. The nature of the data collected in Rahm's multisited ethnography allowed for the unveiling of the similarities in this theme, but also made evident the nuanced ways that this theme presented itself differently across the three sites. The additional potential came through in the varied data Rahm could have delved into and did not. A unique characteristic of ethnographic research, that is not necessarily present in other research methods, is that a wide array of varied data is collected. An analysis of documents, images, and observational field notes, which go beyond interview data, may provide deeper understandings previously untouched in science education.

Structural and Functional Characterization of a Secreted Hookworm Macrophage Migration Inhibitory Factor (MIF) that Interacts with the Human MIF Receptor CD74

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cho,Y.; Jones, B.; Vermeire, J.

2007-01-01

Hookworms, parasitic nematodes that infect nearly one billion people worldwide, are a major cause of anemia and malnutrition. We hypothesize that hookworms actively manipulate the host immune response through the production of specific molecules designed to facilitate infection by larval stages and adult worm survival within the intestine. A full-length cDNA encoding a secreted orthologue of the human cytokine, Macrophage Migration Inhibitory Factor (MIF) has been cloned from the hookworm Ancylostoma ceylanicum. Elucidation of the three-dimensional crystal structure of recombinant AceMIF (rAceMIF) revealed an overall structural homology with significant differences in the tautomerase sites of the human and hookworm proteins.more » The relative bioactivities of human and hookworm MIF proteins were compared using in vitro assays of tautomerase activity, macrophage migration, and binding to MIF receptor CD74. The activity of rAceMIF was not inhibited by the ligand ISO-1, which was previously determined to be an inhibitor of the catalytic site of human MIF. These data define unique immunological, structural, and functional characteristics of AceMIF, thereby establishing the potential for selectively inhibiting the hookworm cytokine as a means of reducing parasite survival and disease pathogenesis.« less

How spatio-temporal habitat connectivity affects amphibian genetic structure

USGS Publications Warehouse

Watts, Alexander G.; Schlichting, P; Billerman, S; Jesmer, B; Micheletti, S; Fortin, M.-J.; Funk, W.C.; Hapeman, P; Muths, Erin L.; Murphy, M.A.

2015-01-01

Heterogeneous landscapes and fluctuating environmental conditions can affect species dispersal, population genetics, and genetic structure, yet understanding how biotic and abiotic factors affect population dynamics in a fluctuating environment is critical for species management. We evaluated how spatio-temporal habitat connectivity influences dispersal and genetic structure in a population of boreal chorus frogs (Pseudacris maculata) using a landscape genetics approach. We developed gravity models to assess the contribution of various factors to the observed genetic distance as a measure of functional connectivity. We selected (a) wetland (within-site) and (b) landscape matrix (between-site) characteristics; and (c) wetland connectivity metrics using a unique methodology. Specifically, we developed three networks that quantify wetland connectivity based on: (i) P. maculata dispersal ability, (ii) temporal variation in wetland quality, and (iii) contribution of wetland stepping-stones to frog dispersal. We examined 18 wetlands in Colorado, and quantified 12 microsatellite loci from 322 individual frogs. We found that genetic connectivity was related to topographic complexity, within- and between-wetland differences in moisture, and wetland functional connectivity as contributed by stepping-stone wetlands. Our results highlight the role that dynamic environmental factors have on dispersal-limited species and illustrate how complex asynchronous interactions contribute to the structure of spatially-explicit metapopulations.

Severe Unresponsive Hypoglycemia Associated with Neuroendocrine Tumor of Unknown Primary Site - 18 Years after Rectal Cancer Surgery. Case Report.

PubMed

Rusu, Octavia Cristina; Costea, Radu Virgil; Popa, Cristian Constantin; Iliesiu, Andreea; Dumitru, Adrian; Becheanu, Gabriel; Neagu, Stefan Ilie

2015-09-01

Neuroendocrine tumors are derived from cells that have the unique ability to synthesize, store and secrete a variety of metabolically active substances, peptides and amines, characteristic of the tissue of origin, which can cause distinct clinical syndromes. We present the case of a 58-year-old patient diagnosed and surgically treated in January 1996 for stage III inferior rectal cancer, who was readmitted after 18 years presenting persistent diarrheic syndrome and asthenia. Investigations performed (abdominal CT) showed multiple liver metastases, initially suspected as being related to the rectal cancer. Biopsy of liver metastases and pathological and immunohistochemical analysis demonstrated the neuroendocrine origin (moderately differentiated neuroendocrine tumor). Seven months after the identification of liver metastases and after initiation of oncological therapy with Interferon and Somatostatin, the patient presented severe hypoglycemia (serum glucose 13-70 mg/dl) proved to be due to insulin-like factors (serum insulin level 64.9 ìU/ml) secreted by metastases. Due to the aggressive evolution of neuroendocrine tumor, with multiple episodes of severe hypoglycemia, resistant to treatment, the patient died approximately one month after the occurrence of hypoglycemic episodes. Despite comprehensive tests (abdominal CT scan, colonoscopy, bone scintigraphy and PET/CT), the primary site of the neuroendocrine tumors remained unknown.

Bench-Scale and Pilot-Scale Treatment Technologies for the ...

EPA Pesticide Factsheets

Coal mine water (CMW) is typically treated to remove suspended solids, acidity, and soluble metals, but high concentrations of total dissolved solids (TDS) have been reported to impact the environment at several CMW discharge points. Consequently, various states have established TDS wastewater regulations and the US EPA has proposed a benchmark conductivity limit to reduce TDS impacts in streams near mining sites. Traditional CMW treatment effectively removes some TDS components, but is not effective in removing major salt ions due to their higher solubility. This paper describes the basic principles, effectiveness, advantages and disadvantages of various TDS removal technologies (adsorption, bioremediation, capacitive deionization, desalination, electro-chemical ion exchange, electrocoagulation, electrodialysis, ion exchange, membrane filtration, precipitation, and reverse osmosis) that have at least been tested in bench- and pilot-scale experiments. Recent discussions about new regulations to include total dissolved solids TDS) limits would propel interest in the TDS removal technologies focused on coal mine water. TDS removal is not a new concept and has been developed using different technologies for a number of applications, but coal mine water has unique characteristics (depending on the site, mining process, and solid-water-oxygen interactions), which make it unlikely to have a single technology predominating over others. What are some novel technolog

Invasion and morphological variation of the non-indigenous barnacle Chthamalus challengeri (Hoek, 1883) in Yangshan Port and its surrounding areas

NASA Astrophysics Data System (ADS)

Liu, Yan; Xue, Junzeng; Lin, Junda; Wu, Huixian

2015-06-01

Invasive species generally possess unique characteristics that allow them to survive the invasion process in order to establish and spread in new habitats. Successful invaders must resist both physical and physiological stresses associated with the changing environment. A common littoral barnacle, Chthamalus challengeri Hoek, 1883 (Crustacea, Cirripedia), which is native to Japan, South Korea and northern China, has become established in the high-littoral zone adjacent to Yangshan Port, Shanghai, China. A comparison of the morphology of Chthamlus species from Zhoushan archipelago with previous description indicates the occurrence of C. challengeri. The new immigrant becomes a dominant species in certain high-intertidal habitats of the adjacent area to of Yangshan Port. C. challengeri was found in part of sampling sites in Zhoushan in 2010; however, it dispersed to all the eleven sampling sites in 2012. Densities of C.challengeri had increased over 10 times in the last 2 years, with the highest mean value reaching 39533 ± 6243 ind. m-2 in the new habitat. The specific ratios of both operculum area ( Sa) to base area ( SA) and average height of parietal plates ( H) to length of base ( L) revealed that C. challengeri displays morphological changes to resist stronger currents in the new habitats for invasion.

Unique local bone tissue characteristics in iliac crest bone biopsy from adolescent idiopathic scoliosis with severe spinal deformity

PubMed Central

Wang, Zhiwei; Chen, Huanxiong; Yu, Y. Eric; Zhang, Jiajun; Cheuk, Ka-Yee; Ng, Bobby K. W.; Qiu, Yong; Guo, X. Edward; Cheng, Jack C. Y.; Lee, Wayne Y. W.

2017-01-01

Adolescent idiopathic scoliosis is a complex disease with unclear etiopathogenesis. Systemic and persistent low bone mineral density is an independent prognostic factor for curve progression. The fundamental question of how bone quality is affected in AIS remains controversy because there is lack of site-matched control for detailed analysis on bone-related parameters. In this case-control study, trabecular bone biopsies from iliac crest were collected intra-operatively from 28 severe AIS patients and 10 matched controls with similar skeletal and sexual maturity, anthropometry and femoral neck BMD Z-score to control confounding effects. In addition to static histomorphometry, micro-computed tomography (μCT) and real time-PCR (qPCR) analyses, individual trabecula segmentation (ITS)-based analysis, finite element analysis (FEA), energy dispersive X-ray spectroscopy (EDX) were conducted to provide advanced analysis of structural, mechanical and mineralization features. μCT and histomorphometry showed consistently reduced trabecular number and connectivity. ITS revealed predominant change in trabecular rods, and EDX confirmed less mineralization. The structural and mineralization abnormality led to slight reduction in apparent modulus, which could be attributed to differential down-regulation of Runx2, and up-regulation of Spp1 and TRAP. In conclusion, this is the first comprehensive study providing direct evidence of undefined unique pathological changes at different bone hierarchical levels in AIS. PMID:28054655

Emerging 0D Transition-Metal Dichalcogenides for Sensors, Biomedicine, and Clean Energy.

PubMed

Li, Bang Lin; Setyawati, Magdiel Inggrid; Zou, Hao Lin; Dong, Jiang Xue; Luo, Hong Qun; Li, Nian Bing; Leong, David Tai

2017-08-01

Following research on two-dimensional (2D) transition metal dichalcogenides (TMDs), zero-dimensional (0D) TMDs nanostructures have also garnered some attention due to their unique properties; exploitable for new applications. The 0D TMDs nanostructures stand distinct from their larger 2D TMDs cousins in terms of their general structure and properties. 0D TMDs possess higher bandgaps, ultra-small sizes, high surface-to-volume ratios with more active edge sites per unit mass. So far, reported 0D TMDs can be mainly classified as quantum dots, nanodots, nanoparticles, and small nanoflakes. All exhibited diverse applications in various fields due to their unique and excellent properties. Of significance, through exploiting inherent characteristics of 0D TMDs materials, enhanced catalytic, biomedical, and photoluminescence applications can be realized through this exciting sub-class of TMDs. Herein, we comprehensively review the properties and synthesis methods of 0D TMDs nanostructures and focus on their potential applications in sensor, biomedicine, and energy fields. This article aims to educate potential adopters of these excitingly new nanomaterials as well as to inspire and promote the development of more impactful applications. Especially in this rapidly evolving field, this review may be a good resource of critical insights and in-depth comparisons between the 0D and 2D TMDs. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

The Coevolution of Life and Environment on Mars: An Ecosystem Perspective on the Robotic Exploration of Biosignatures.

PubMed

Cabrol, Nathalie A

2018-01-01

Earth's biological and environmental evolution are intertwined and inseparable. This coevolution has become a fundamental concept in astrobiology and is key to the search for life beyond our planet. In the case of Mars, whether a coevolution took place is unknown, but analyzing the factors at play shows the uniqueness of each planetary experiment regardless of similarities. Early Earth and early Mars shared traits. However, biological processes on Mars, if any, would have had to proceed within the distinctive context of an irreversible atmospheric collapse, greater climate variability, and specific planetary characteristics. In that, Mars is an important test bed for comparing the effects of a unique set of spatiotemporal changes on an Earth-like, yet different, planet. Many questions remain unanswered about Mars' early environment. Nevertheless, existing data sets provide a foundation for an intellectual framework where notional coevolution models can be explored. In this framework, the focus is shifted from planetary-scale habitability to the prospect of habitats, microbial ecotones, pathways to biological dispersal, biomass repositories, and their meaning for exploration. Critically, as we search for biosignatures, this focus demonstrates the importance of starting to think of early Mars as a biosphere and vigorously integrating an ecosystem approach to landing site selection and exploration. Key Words: Astrobiology-Biosignatures-Coevolution of Earth and life-Mars. Astrobiology 18, 1-27.

The Coevolution of Life and Environment on Mars: An Ecosystem Perspective on the Robotic Exploration of Biosignatures

PubMed Central

2018-01-01

Abstract Earth's biological and environmental evolution are intertwined and inseparable. This coevolution has become a fundamental concept in astrobiology and is key to the search for life beyond our planet. In the case of Mars, whether a coevolution took place is unknown, but analyzing the factors at play shows the uniqueness of each planetary experiment regardless of similarities. Early Earth and early Mars shared traits. However, biological processes on Mars, if any, would have had to proceed within the distinctive context of an irreversible atmospheric collapse, greater climate variability, and specific planetary characteristics. In that, Mars is an important test bed for comparing the effects of a unique set of spatiotemporal changes on an Earth-like, yet different, planet. Many questions remain unanswered about Mars' early environment. Nevertheless, existing data sets provide a foundation for an intellectual framework where notional coevolution models can be explored. In this framework, the focus is shifted from planetary-scale habitability to the prospect of habitats, microbial ecotones, pathways to biological dispersal, biomass repositories, and their meaning for exploration. Critically, as we search for biosignatures, this focus demonstrates the importance of starting to think of early Mars as a biosphere and vigorously integrating an ecosystem approach to landing site selection and exploration. Key Words: Astrobiology—Biosignatures—Coevolution of Earth and life—Mars. Astrobiology 18, 1–27. PMID:29252008

Archaeological Feedback as a Research Methodology in Near-Surface Geophysics

NASA Astrophysics Data System (ADS)

Maillol, J.; Ortega-Ramírez, J.; Berard, B.

2005-05-01

A unique characteristic of archaeological geophysics is to present the researchers in applied geophysics with the opportunity to verify their interpretation of geophysical data through the direct observation of often extremely detailed excavations. This is usually known as archaeological feedback. Archaeological materials have been slowly buried over periods ranging from several hundreds to several thousands of years, undergoing natural sedimentary and soil-forming processes. Once excavated, archaeological features therefore constitute more realistic test subjects than the targets artifically buried in common geophysical test sites. We are presenting the outcome of several such verification tests aimed at clarifying issues in geometry and spatial resolution of ground penetrating radar (GPR) images. On the site of a Roman villa in SE Portugal 500 Mhz GPR images are shown to depict very accurately the position and geometry of partially excavated remains. In the Maya city of Palenque, Mexico, 900 Mhz data allows the depth of tombs and natural cavities to be determined with cm accuracy. The predicted lateral extent of the cavities is more difficult to match with the reality due to the cluttering caused by high frequency. In the rainforest of Western Africa, 500 MHz GPR was used to prospect for stone tool sites. When very careful positioning and high density data sampling is achieved, stones can be accurately located and retrieved at depths exceeding 1 m with maximum positioning errors of 12cm horizontally and 2 cm vertically. In more difficult data collection conditions however, errors in positioning are shown to actually largely exceed the predictions based on quantitative theoretical resolution considerations. Geophysics has long been recognized as a powerful tool for prospecting and characterizing archaeological sites. Reciprocally, these results show that archaeology is an unparalleled test environment for the assesment and development of high resolution geophysical methods.

A degree-day model of sheep grazing influence on alfalfa weevil and crop characteristics.

PubMed

Goosey, Hayes B

2012-02-01

Domestic sheep (Ovis spp.) grazing is emerging as an integrated pest management tactic for alfalfa weevil, Hypera postica (Gyllenhal), management and a degree-day model is needed as a decision and support tool. In response to this need, grazing exclosures with unique degree-days and stocking rates were established at weekly intervals in a central Montana alfalfa field during 2008 and 2009. Analyses indicate that increased stocking rates and grazing degree-days were associated with decreased crop levels of weevil larvae. Larval data collected from grazing treatments were regressed against on-site and near-site temperatures that produced the same accuracy. The near-site model was chosen to encourage producer acceptance. The regression slope differed from zero, had an r2 of 0.83, and a root mean square error of 0.2. Crop data were collected to achieve optimal weevil management with forage quality and yield. Differences were recorded in crude protein, acid and neutral detergent fibers, total digestible nutrients, and mean stage by weight. Stem heights differed with higher stocking rates and degree-days recording the shortest alfalfa canopy height at harvest. The degree-day model was validated at four sites during 2010 with a mean square prediction error of 0.74. The recommendation from this research is to stock alfalfa fields in the spring before 63 DD with rates between 251 and 583 sheep days per hectare (d/ha). Sheep should be allowed to graze to a minimum of 106 and maximum of 150 DD before removal. This model gives field entomologists a new method for implementing grazing in an integrated pest management program.

Experience of Primary Care among Homeless Individuals with Mental Health Conditions

PubMed Central

Chrystal, Joya G.; Glover, Dawn L.; Young, Alexander S.; Whelan, Fiona; Austin, Erika L.; Johnson, Nancy K.; Pollio, David E.; Holt, Cheryl L.; Stringfellow, Erin; Gordon, Adam J.; Kim, Theresa A.; Daigle, Shanette G.; Steward, Jocelyn L.; Kertesz, Stefan G

2015-01-01

The delivery of primary care to homeless individuals with mental health conditions presents unique challenges. To inform healthcare improvement, we studied predictors of favorable primary care experience among homeless persons with mental health conditions treated at sites that varied in degree of homeless-specific service tailoring. This was a multi-site, survey-based comparison of primary care experiences at three mainstream primary care clinics of the Veterans Administration (VA), one homeless-tailored VA clinic, and one tailored non-VA healthcare program. Persons who accessed primary care service two or more times from July 2008 through June 2010 (N = 366) were randomly sampled. Predictor variables included patient and organization characteristics suggested by the patient perception model developed by Sofaer and Firminger (2005), with an emphasis on mental health. The primary care experience was assessed with the Primary Care Quality-Homeless (PCQ-H) questionnaire, a validated survey instrument. Multiple regression identified predictors of positive experiences (i.e. higher PCQ-H total score). Significant predictors of a positive experience included a site offering tailored service design, perceived choice among providers, and currently domiciled status. There was an interaction effect between site and severe psychiatric symptoms. For persons with severe psychiatric symptoms, a homeless-tailored service design was significantly associated with a more favorable primary care experience. For persons without severe psychiatric symptoms, this difference was not significant. This study supports the importance of tailored healthcare delivery designed for homeless persons’ needs, with such services potentially holding special relevance for persons with mental health conditions. To improve patient experience among the homeless, organizations may want to deliver services that are tailored to homelessness and offer a choice of providers. PMID:25659142

Experience of primary care among homeless individuals with mental health conditions.

PubMed

Chrystal, Joya G; Glover, Dawn L; Young, Alexander S; Whelan, Fiona; Austin, Erika L; Johnson, Nancy K; Pollio, David E; Holt, Cheryl L; Stringfellow, Erin; Gordon, Adam J; Kim, Theresa A; Daigle, Shanette G; Steward, Jocelyn L; Kertesz, Stefan G

2015-01-01

The delivery of primary care to homeless individuals with mental health conditions presents unique challenges. To inform healthcare improvement, we studied predictors of favorable primary care experience among homeless persons with mental health conditions treated at sites that varied in degree of homeless-specific service tailoring. This was a multi-site, survey-based comparison of primary care experiences at three mainstream primary care clinics of the Veterans Administration (VA), one homeless-tailored VA clinic, and one tailored non-VA healthcare program. Persons who accessed primary care service two or more times from July 2008 through June 2010 (N = 366) were randomly sampled. Predictor variables included patient and organization characteristics suggested by the patient perception model developed by Sofaer and Firminger (2005), with an emphasis on mental health. The primary care experience was assessed with the Primary Care Quality-Homeless (PCQ-H) questionnaire, a validated survey instrument. Multiple regression identified predictors of positive experiences (i.e. higher PCQ-H total score). Significant predictors of a positive experience included a site offering tailored service design, perceived choice among providers, and currently domiciled status. There was an interaction effect between site and severe psychiatric symptoms. For persons with severe psychiatric symptoms, a homeless-tailored service design was significantly associated with a more favorable primary care experience. For persons without severe psychiatric symptoms, this difference was not significant. This study supports the importance of tailored healthcare delivery designed for homeless persons' needs, with such services potentially holding special relevance for persons with mental health conditions. To improve patient experience among the homeless, organizations may want to deliver services that are tailored to homelessness and offer a choice of providers.

Three years of hillslope sediment yields following the Valley Complex fires, western Montana

Treesearch

Peter R. Robichaud; Joseph W. Wagenbrenner; Robert E. Brown; Kevin M. Spigel

2009-01-01

The 2000 Bitterroot Valley wildfires provided an opportunity to measure post-fire effects and recovery rates. We established 24 small (0.01 ha [0.02 acre]) plots in four high-severity burn sites. We measured sediment yields at each site with silt fences. We also measured rainfall characteristics, soil water repellency, vegetative cover, and other site characteristics....

Sentiment Analysis of Web Sites Related to Vaginal Mesh Use in Pelvic Reconstructive Surgery.

PubMed

Hobson, Deslyn T G; Meriwether, Kate V; Francis, Sean L; Kinman, Casey L; Stewart, J Ryan

2018-05-02

The purpose of this study was to utilize sentiment analysis to describe online opinions toward vaginal mesh. We hypothesized that sentiment in legal Web sites would be more negative than that in medical and reference Web sites. We generated a list of relevant key words related to vaginal mesh and searched Web sites using the Google search engine. Each unique uniform resource locator (URL) was sorted into 1 of 6 categories: "medical", "legal", "news/media", "patient generated", "reference", or "unrelated". Sentiment of relevant Web sites, the primary outcome, was scored on a scale of -1 to +1, and mean sentiment was compared across all categories using 1-way analysis of variance. Tukey test evaluated differences between category pairs. Google searches of 464 unique key words resulted in 11,405 URLs. Sentiment analysis was performed on 8029 relevant URLs (3472 legal, 1625 "medical", 1774 "reference", 666 "news media", 492 "patient generated"). The mean sentiment for all relevant Web sites was +0.01 ± 0.16; analysis of variance revealed significant differences between categories (P < 0.001). Web sites categorized as "legal" and "news/media" had a slightly negative mean sentiment, whereas those categorized as "medical," "reference," and "patient generated" had slightly positive mean sentiments. Tukey test showed differences between all category pairs except the "medical" versus "reference" in comparison with the largest mean difference (-0.13) seen in the "legal" versus "reference" comparison. Web sites related to vaginal mesh have an overall mean neutral sentiment, and Web sites categorized as "medical," "reference," and "patient generated" have significantly higher sentiment scores than related Web sites in "legal" and "news/media" categories.

Site transition framework for long-term surveillance and maintenance

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

2014-04-01

This document provides a framework for all U.S. Department of Energy (DOE) facilities and sites where DOE may have anticipated long-term surveillance and maintenance (LTSM) responsibilities. It is a tool to help facilitate a smooth transition from remediation to LTSM, providing a systematic process for affected parties to utilize in analyzing the baseline to understand and manage the actions from EM mission completion through a site’s transition into LTSM. The framework is not meant to provide an exhaustive list of the specific requirement and information that are needed. Sites will have unique considerations that may not be adequately addressed bymore » this tool, and it is anticipated that a team comprised of the transferring and receiving organization will use judgment in utilizing this augmenting with other DOE guidance. However the framework should be followed to the extent possible at each site; and adapted to accommodate unique site-specific requirements, needs, and documents. Since the objective of the tool is facilitate better understanding of the conditions of the site and the actions required for transfer, the transition team utilizing the checklist is expected to consult with management of both the receiving and transferring organization to verify that major concerns are addressed. Ideally, this framework should be used as early in the remediation process as possible. Subsequent applications of the Site Transition Framework (STF) to the site should be conducted periodically and used to verify that all appropriate steps have been or will be taken to close-out the site and that actions by both organization are identified to transfer the site to LTSM. The requirements are provided herein.« less

Remodeling characteristics and collagen distribution in synthetic mesh materials explanted from human subjects after abdominal wall reconstruction: an analysis of remodeling characteristics by patient risk factors and surgical site classifications

PubMed Central

Cavallo, Jaime A.; Roma, Andres A.; Jasielec, Mateusz S.; Ousley, Jenny; Creamer, Jennifer; Pichert, Matthew D.; Baalman, Sara; Frisella, Margaret M.; Matthews, Brent D.

2014-01-01

Background The purpose of this study was to evaluate the associations between patient characteristics or surgical site classifications and the histologic remodeling scores of synthetic meshes biopsied from their abdominal wall repair sites in the first attempt to generate a multivariable risk prediction model of non-constructive remodeling. Methods Biopsies of the synthetic meshes were obtained from the abdominal wall repair sites of 51 patients during a subsequent abdominal re-exploration. Biopsies were stained with hematoxylin and eosin, and evaluated according to a semi-quantitative scoring system for remodeling characteristics (cell infiltration, cell types, extracellular matrix deposition, inflammation, fibrous encapsulation, and neovascularization) and a mean composite score (CR). Biopsies were also stained with Sirius Red and Fast Green, and analyzed to determine the collagen I:III ratio. Based on univariate analyses between subject clinical characteristics or surgical site classification and the histologic remodeling scores, cohort variables were selected for multivariable regression models using a threshold p value of ≤0.200. Results The model selection process for the extracellular matrix score yielded two variables: subject age at time of mesh implantation, and mesh classification (c-statistic = 0.842). For CR score, the model selection process yielded two variables: subject age at time of mesh implantation and mesh classification (r2 = 0.464). The model selection process for the collagen III area yielded a model with two variables: subject body mass index at time of mesh explantation and pack-year history (r2 = 0.244). Conclusion Host characteristics and surgical site assessments may predict degree of remodeling for synthetic meshes used to reinforce abdominal wall repair sites. These preliminary results constitute the first steps in generating a risk prediction model that predicts the patients and clinical circumstances for which non-constructive remodeling of an abdominal wall repair site with synthetic mesh reinforcement is most likely to occur. PMID:24442681

Exploring the Real Thing: A Guide to Educational Programs at National Park Sites in Massachusetts.

ERIC Educational Resources Information Center

Emerson, Rae; Hoermann, Elizabeth; Stearns, Liza

As the steward of the nation's premier historic, natural, and recreational resources, the National Park Service manages 15 unique areas within the Commonwealth of Massachusetts. Each of these sites offers hands-on programs specifically geared to meet the needs of teachers and students. The programs support the Massachusetts Curriculum Frameworks…

Stopping Web Plagiarists from Stealing Your Content

ERIC Educational Resources Information Center

Goldsborough, Reid

2004-01-01

This article gives tips on how to avoid having content stolen by plagiarists. Suggestions include: using a Web search service such as Google to search for unique strings of text at the individuals site to uncover other sites with the same content; buying a infringement-detection program; or hiring a public relations firm to do the work. There are…

Wilderness stewardship challenges in the uKhahlamba Drakensberg Park World Heritage Site

Treesearch

Sonja Krüger

2007-01-01

The location of the uKhahlamba Drakensberg Park wilderness areas along an international border and within a World Heritage Site and Transfrontier Conservation Area, provides unique opportunities and challenges for the stewardship of these areas. Although the wilderness areas were proclaimed more than 30 years ago, wilderness-specific planning, management and monitoring...

Nucleotide and deduced amino acid sequence of the envelope gene of the Vasilchenko strain of TBE virus; comparison with other flaviviruses.

PubMed

Gritsun, T S; Frolova, T V; Pogodina, V V; Lashkevich, V A; Venugopal, K; Gould, E A

1993-02-01

A strain of tick-borne encephalitis virus known as Vasilchenko (Vs) exhibits relatively low virulence characteristics in monkeys, Syrian hamsters and humans. The gene encoding the envelope glycoprotein of this virus was cloned and sequenced. Alignment of the sequence with those of other known tick-borne flaviviruses and identification of the recognised amino acid genetic marker EHLPTA confirmed its identity as a member of the TBE complex. However, Vs virus was distinguishable from eastern and western tick-borne serotypes by the presence of the sequence AQQ at amino acid positions 232-234 and also by the presence of other specific amino acid substitutions which may be genetic markers for these viruses and could determine their pathogenetic characteristics. When compared with other tick-borne flaviviruses, Vs virus had 12 unique amino acid substitutions including an additional potential glycosylation site at position (315-317). The Vs virus strain shared closest nucleotide and amino acid homology (84.5% and 95.5% respectively) with western and far eastern strains of tick-borne encephalitis virus. Comparison with the far eastern serotype of tick-borne encephalitis virus, by cross-immunoelectrophoresis of Vs virions and PAGE analysis of the extracted virion proteins, revealed differences in surface charge and virus stability that may account for the different virulence characteristics of Vs virus. These results support and enlarge upon previous data obtained from molecular and serological analysis.

Characteristics of successful puma kill sites of elk in the Black Hills, South Dakota

Treesearch

Chadwick P. Lehman; Christopher T. Rota; Mark A. Rumble; Joshua J. Millspaugh

2017-01-01

Elk Cervus canadensis nelsoni in the Black Hills, South Dakota, have been declining since 2006 and there is concern by resource managers and hunters that puma Puma concolor predation may be contributing to declining herds. We evaluated characteristics at sites where puma successfully killed elk in the Black Hills of South Dakota. We evaluated characteristics at coarse...

Identification of high-efficiency 3'GG gRNA motifs in indexed FASTA files with ngg2.

PubMed

Roberson, Elisha D O

CRISPR/Cas9 is emerging as one of the most-used methods of genome modification in organisms ranging from bacteria to human cells. However, the efficiency of editing varies tremendously site-to-site. A recent report identified a novel motif, called the 3'GG motif, which substantially increases the efficiency of editing at all sites tested in C. elegans . Furthermore, they highlighted that previously published gRNAs with high editing efficiency also had this motif. I designed a python command-line tool, ngg2, to identify 3'GG gRNA sites from indexed FASTA files. As a proof-of-concept, I screened for these motifs in six model genomes: Saccharomyces cerevisiae , Caenorhabditis elegans , Drosophila melanogaster , Danio rerio , Mus musculus , and Homo sapiens. I also scanned the genomes of pig ( Sus scrofa ) and African elephant ( Loxodonta africana ) to demonstrate the utility in non-model organisms. I identified more than 60 million single match 3'GG motifs in these genomes. Greater than 61% of all protein coding genes in the reference genomes had at least one unique 3'GG gRNA site overlapping an exon. In particular, more than 96% of mouse and 93% of human protein coding genes have at least one unique, overlapping 3'GG gRNA. These identified sites can be used as a starting point in gRNA selection, and the ngg2 tool provides an important ability to identify 3'GG editing sites in any species with an available genome sequence.

Lesion bypass activity of DNA polymerase θ (POLQ) is an intrinsic property of the pol domain and depends on unique sequence inserts.

PubMed

Hogg, Matthew; Seki, Mineaki; Wood, Richard D; Doublié, Sylvie; Wallace, Susan S

2011-01-21

DNA polymerase θ (POLQ, polθ) is a large, multidomain DNA polymerase encoded in higher eukaryotic genomes. It is important for maintaining genetic stability in cells and helping protect cells from DNA damage caused by ionizing radiation. POLQ contains an N-terminal helicase-like domain, a large central domain of indeterminate function, and a C-terminal polymerase domain with sequence similarity to the A-family of DNA polymerases. The enzyme has several unique properties, including low fidelity and the ability to insert and extend past abasic sites and thymine glycol lesions. It is not known whether the abasic site bypass activity is an intrinsic property of the polymerase domain or whether helicase activity is also required. Three "insertion" sequence elements present in POLQ are not found in any other A-family DNA polymerase, and it has been proposed that they may lend some unique properties to POLQ. Here, we analyzed the activity of the DNA polymerase in the absence of each sequence insertion. We found that the pol domain is capable of highly efficient bypass of abasic sites in the absence of the helicase-like or central domains. Insertion 1 increases the processivity of the polymerase but has little, if any, bearing on the translesion synthesis properties of the enzyme. However, removal of insertions 2 and 3 reduces activity on undamaged DNA and completely abrogates the ability of the enzyme to bypass abasic sites or thymine glycol lesions. Copyright © 2010 Elsevier Ltd. All rights reserved.

Lived Experiences of Low Socioeconomic Millennial Generation College Students

ERIC Educational Resources Information Center

Thacker, Kelly L.

2012-01-01

The characteristics and needs of college students across the United States are ever-changing. As Millennial generation students, born between 1982 and 2003 (Howe & Strauss, 2000), attend college, unique characteristics are present. Commonalities within the Millennial generation have been identified; however, socioeconomic status can impact a…

Revealing the Secrets of Stonehenge Through the Application of Laser Scanning, Photogrammetry and Visualisation Techniques

NASA Astrophysics Data System (ADS)

Bryan, P. G.; Abbott, M.; Dodson, A. J.

2013-07-01

Stonehenge is perhaps the most famous prehistoric monument in the world. Begun as a simple earthwork enclosure, it was built in several stages with the unique lintelled stone circle being erected in the Neolithic period in around 2,500 BC. Today Stonehenge, together with Avebury and other associated sites, form the heart of a World Heritage Site (WHS) with a unique and dense concentration of outstanding prehistoric monuments. In 2011 English Heritage (EH) embarked on a new survey of the monument. Undertaken by the Greenhatch Group, a commercial survey company based near Derby, a combination of laser scanning and photogrammetric approaches were used to generate the required scale and detailed level of output required by English Heritage. This paper will describe the background to this project and its context within previous survey activities at this World Heritage Site. It will explain the data acquisition technology and processes undertaken on site, the datasets derived from post-processing and their filtering and analysis within both subsequent research projects. Alongside a description of how the data is currently being exploited and proposed future applications within the conservation and management of the site, it will finish by considering the impact of developing geospatial imaging technologies.

Structural Insights into the Protease-like Antigen Plasmodium falciparum SERA5 and Its Noncanonical Active-Site Serine

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hodder, Anthony N.; Malby, Robyn L.; Clarke, Oliver B.

The sera genes of the malaria-causing parasite Plasmodium encode a family of unique proteins that are maximally expressed at the time of egress of parasites from infected red blood cells. These multi-domain proteins are unique, containing a central papain-like cysteine-protease fragment enclosed between the disulfide-linked N- and C-terminal domains. However, the central fragment of several members of this family, including serine repeat antigen 5 (SERA5), contains a serine (S596) in place of the active-site cysteine. Here we report the crystal structure of the central protease-like domain of Plasmodium falciparum SERA5, revealing a number of anomalies in addition to the putativemore » nucleophilic serine: (1) the structure of the putative active site is not conducive to binding substrate in the canonical cysteine-protease manner; (2) the side chain of D594 restricts access of substrate to the putative active site; and (3) the S{sub 2} specificity pocket is occupied by the side chain of Y735, reducing this site to a small depression on the protein surface. Attempts to determine the structure in complex with known inhibitors were not successful. Thus, despite having revealed its structure, the function of the catalytic domain of SERA5 remains an enigma.« less

Local nutrient regimes determine site-specific environmental triggers of cyanobacterial and microcystin variability in urban lakes

NASA Astrophysics Data System (ADS)

Sinang, S. C.; Reichwaldt, E. S.; Ghadouani, A.

2014-10-01

Toxic cyanobacterial blooms in urban lakes present serious health hazards to humans and animals and require effective management strategies. In the management of toxic cyanobacteria blooms, understanding the roles of environmental factors is crucial. To date, a range of environmental factors have been proposed as potential triggers for the spatiotemporal variability of cyanobacterial biomass and microcystins in freshwater systems. However, the environmental triggers of cyanobacteria and microcystin variability remain a subject of debate due to contrasting findings. This issue has raised the question if the environmental triggers are site-specific and unique between water bodies. In this study, we investigated the site-specificity of environmental triggers for cyanobacterial bloom and cyanotoxins dynamics. Our study suggests that cyanobacterial dominance and cyanobacterial microcystin content variability were significantly correlated to phosphorus and iron concentrations. However, the correlations between phosphorus and iron with cyanobacterial biomass and microcystin variability were not consistent between lakes, thus suggesting a site specificity of these environmental factors. The discrepancies in the correlations could be explained by differences in local nutrient concentration and the cyanobacterial community in the systems. The findings of this study suggest that identification of site-specific environmental factors under unique local conditions is an important strategy to enhance positive outcomes in cyanobacterial bloom control measures.

Are seed and dispersal characteristics of plants capable of predicting colonization of post-mining sites?

PubMed

Horáčková, Martina; Řehounková, Klára; Prach, Karel

2016-07-01

Seed characteristics play an important role in the colonization and subsequent persistence of species during succession in disturbed sites and thus may contribute to being able to predict restoration success. In the present study, we investigated how various seed characteristics participated in 11 spontaneous successional series running in different mining sites (spoil heaps, extracted sand and sand-gravel pits, extracted peatlands, and stone quarries) in the Czech Republic, Central Europe. Using 1864 samples from 1- to 100-years-old successional stages, we tested whether species optimum along the succession gradient could be predicted using 10 basic species traits connected with diaspores and dispersal. Seed longevity, diaspore mass, endozoochory, and autochory appeared to be the best predictors. The results indicate that seed characteristics can predict to a certain degree spontaneous vegetation succession, i.e., passive restoration, in the mining sites. A screening of species available in the given landscape (regional and local species pools) may help to identify those species which would potentially colonize the disturbed sites. Extensive databases of species traits, nowadays available for the Central European flora, enable such screening.

Monolithically Integrated High-β Nanowire Lasers on Silicon.

PubMed

Mayer, B; Janker, L; Loitsch, B; Treu, J; Kostenbader, T; Lichtmannecker, S; Reichert, T; Morkötter, S; Kaniber, M; Abstreiter, G; Gies, C; Koblmüller, G; Finley, J J

2016-01-13

Reliable technologies for the monolithic integration of lasers onto silicon represent the holy grail for chip-level optical interconnects. In this context, nanowires (NWs) fabricated using III-V semiconductors are of strong interest since they can be grown site-selectively on silicon using conventional epitaxial approaches. Their unique one-dimensional structure and high refractive index naturally facilitate low loss optical waveguiding and optical recirculation in the active NW-core region. However, lasing from NWs on silicon has not been achieved to date, due to the poor modal reflectivity at the NW-silicon interface. We demonstrate how, by inserting a tailored dielectric interlayer at the NW-Si interface, low-threshold single mode lasing can be achieved in vertical-cavity GaAs-AlGaAs core-shell NW lasers on silicon as measured at low temperature. By exploring the output characteristics along a detection direction parallel to the NW-axis, we measure very high spontaneous emission factors comparable to nanocavity lasers (β = 0.2) and achieve ultralow threshold pump energies ≤11 pJ/pulse. Analysis of the input-output characteristics of the NW lasers and the power dependence of the lasing emission line width demonstrate the potential for high pulsation rates ≥250 GHz. Such highly efficient nanolasers grown monolithically on silicon are highly promising for the realization of chip-level optical interconnects.

Unique attributes of cyanobacterial metabolism revealed by improved genome-scale metabolic modeling and essential gene analysis

DOE PAGES

Broddrick, Jared T.; Rubin, Benjamin E.; Welkie, David G.; ...

2016-12-20

The model cyanobacterium, Synechococcus elongatus PCC 7942, is a genetically tractable obligate phototroph that is being developed for the bioproduction of high-value chemicals. Genome-scale models (GEMs) have been successfully used to assess and engineer cellular metabolism; however, GEMs of phototrophic metabolism have been limited by the lack of experimental datasets for model validation and the challenges of incorporating photon uptake. In this paper, we develop a GEM of metabolism in S. elongatus using random barcode transposon site sequencing (RB-TnSeq) essential gene and physiological data specific to photoautotrophic metabolism. The model explicitly describes photon absorption and accounts for shading, resulting inmore » the characteristic linear growth curve of photoautotrophs. GEM predictions of gene essentiality were compared with data obtained from recent dense-transposon mutagenesis experiments. This dataset allowed major improvements to the accuracy of the model. Furthermore, discrepancies between GEM predictions and the in vivo dataset revealed biological characteristics, such as the importance of a truncated, linear TCA pathway, low flux toward amino acid synthesis from photorespiration, and knowledge gaps within nucleotide metabolism. Finally, coupling of strong experimental support and photoautotrophic modeling methods thus resulted in a highly accurate model of S. elongatus metabolism that highlights previously unknown areas of S. elongatus biology.« less

National Characteristics of Lymphatic Malformations in Children: Inpatient Estimates and Trends in the United States, 2000 to 2009.

PubMed

Cheng, Jeffrey; Liu, Beiyu; Farjat, Alfredo E; Routh, Jonathan

2018-04-01

With ever increasing demands to manage finite resources for health care utilization, we performed an investigation to identify inpatient clinical characteristics and trends in children with lymphatic malformations using the Kids' Inpatient Database, years 2000 to 2009, to help identify populations best suited for resource deployment. Subjects included children 18 years and below with International Classification of Diseases (ICD), ninth revision code: 228.1-lymphangioma, any site. In the United States, between 2000 and 2009, inpatient pediatric patients with lymphatic malformations most commonly affected children aged 3 years and younger, urban hospital locations, and the South and West regions. There was no significant change in age of children with lymphatic malformations or the distribution of their age from year to year, P=0.948 and 0.4223, respectively. No significant evidence for seasonal variation or effect on inpatient admission was identified, P=0.7071. A great majority of admissions (>96%) were in urban locations across each year. There was also no significant change in breakdown of admissions by geographic location, P=0.7133. Further investigation may help to elucidate how to improve access to multidisciplinary vascular anomalies teams to optimize care for these children with unique and complex lymphatic malformations.

Unique attributes of cyanobacterial metabolism revealed by improved genome-scale metabolic modeling and essential gene analysis

PubMed Central

Broddrick, Jared T.; Rubin, Benjamin E.; Welkie, David G.; Du, Niu; Mih, Nathan; Diamond, Spencer; Lee, Jenny J.; Golden, Susan S.; Palsson, Bernhard O.

2016-01-01

The model cyanobacterium, Synechococcus elongatus PCC 7942, is a genetically tractable obligate phototroph that is being developed for the bioproduction of high-value chemicals. Genome-scale models (GEMs) have been successfully used to assess and engineer cellular metabolism; however, GEMs of phototrophic metabolism have been limited by the lack of experimental datasets for model validation and the challenges of incorporating photon uptake. Here, we develop a GEM of metabolism in S. elongatus using random barcode transposon site sequencing (RB-TnSeq) essential gene and physiological data specific to photoautotrophic metabolism. The model explicitly describes photon absorption and accounts for shading, resulting in the characteristic linear growth curve of photoautotrophs. GEM predictions of gene essentiality were compared with data obtained from recent dense-transposon mutagenesis experiments. This dataset allowed major improvements to the accuracy of the model. Furthermore, discrepancies between GEM predictions and the in vivo dataset revealed biological characteristics, such as the importance of a truncated, linear TCA pathway, low flux toward amino acid synthesis from photorespiration, and knowledge gaps within nucleotide metabolism. Coupling of strong experimental support and photoautotrophic modeling methods thus resulted in a highly accurate model of S. elongatus metabolism that highlights previously unknown areas of S. elongatus biology. PMID:27911809

Large Historical Earthquakes and Tsunami Hazards in the Western Mediterranean: Source Characteristics and Modelling

NASA Astrophysics Data System (ADS)

Harbi, Assia; Meghraoui, Mustapha; Belabbes, Samir; Maouche, Said

2010-05-01

The western Mediterranean region was the site of numerous large earthquakes in the past. Most of these earthquakes are located at the East-West trending Africa-Eurasia plate boundary and along the coastline of North Africa. The most recent recorded tsunamigenic earthquake occurred in 2003 at Zemmouri-Boumerdes (Mw 6.8) and generated ~ 2-m-high tsunami wave. The destructive wave affected the Balearic Islands and Almeria in southern Spain and Carloforte in southern Sardinia (Italy). The earthquake provided a unique opportunity to gather instrumental records of seismic waves and tide gauges in the western Mediterranean. A database that includes a historical catalogue of main events, seismic sources and related fault parameters was prepared in order to assess the tsunami hazard of this region. In addition to the analysis of the 2003 records, we study the 1790 Oran and 1856 Jijel historical tsunamigenic earthquakes (Io = IX and X, respectively) that provide detailed observations on the heights and extension of past tsunamis and damage in coastal zones. We performed the modelling of wave propagation using NAMI-DANCE code and tested different fault sources from synthetic tide gauges. We observe that the characteristics of seismic sources control the size and directivity of tsunami wave propagation on both northern and southern coasts of the western Mediterranean.

Analysis of trace levels of sulfonamide and tetracycline antimicrobials in groundwater and surface water using solid-phase extraction and liquid chromatography/mass spectrometry

USGS Publications Warehouse

Lindsey, M.E.; Meyer, M.; Thurman, E.M.

2001-01-01

A method has been developed for the trace analysis of two classes of antimicrobials consisting of six sulfonamides (SAs) and five tetracyclines (TCs), which commonly are used for veterinary purposes and agricultural feed additives and are suspected to leach into ground and surface water. The method used solid-phase extraction and liquid chromatography/mass spectrometry (LC/MS) with positive ion electrospray. The unique combination of a metal chelation agent (Na2EDTA) with a macroporous copolymer resulted in quantitative recoveries by solid-phase extraction (mean recovery, 98 ?? 12%) at submicrogram-per-liter concentrations. An ammonium formate/formic acid buffer with a methanol/water gradient was used to separate the antimicrobials and to optimize the signal intensity. Mass spectral fragmentation and ionization characteristics were determined for each class of compounds for unequivocal identification. For all SAs, a characteristic m/z 156 ion representing the sulfanilyl fragment was identified. TCs exhibited neutral losses of 17 amu resulting from the loss of ammonia and 35 amu from the subsequent loss of water. Unusual matrix effects were seen only for TCs in this first survey of groundwater and surface water samples from sites around the United States, requiring that TCs be quantitated using the method of standard additions.

Exciton-to-Dopant Energy Transfer in Mn-Doped Cesium Lead Halide Perovskite Nanocrystals.

PubMed

Parobek, David; Roman, Benjamin J; Dong, Yitong; Jin, Ho; Lee, Elbert; Sheldon, Matthew; Son, Dong Hee

2016-12-14

We report the one-pot synthesis of colloidal Mn-doped cesium lead halide (CsPbX 3 ) perovskite nanocrystals and efficient intraparticle energy transfer between the exciton and dopant ions resulting in intense sensitized Mn luminescence. Mn-doped CsPbCl 3 and CsPb(Cl/Br) 3 nanocrystals maintained the same lattice structure and crystallinity as their undoped counterparts with nearly identical lattice parameters at ∼0.2% doping concentrations and no signature of phase separation. The strong sensitized luminescence from d-d transition of Mn 2+ ions upon band-edge excitation of the CsPbX 3 host is indicative of sufficiently strong exchange coupling between the charge carriers of the host and dopant d electrons mediating the energy transfer, essential for obtaining unique properties of magnetically doped quantum dots. Highly homogeneous spectral characteristics of Mn luminescence from an ensemble of Mn-doped CsPbX 3 nanocrystals and well-defined electron paramagnetic resonance spectra of Mn 2+ in host CsPbX 3 nanocrystal lattices suggest relatively uniform doping sites, likely from substitutional doping at Pb 2+ . These observations indicate that CsPbX 3 nanocrystals, possessing many superior optical and electronic characteristics, can be utilized as a new platform for magnetically doped quantum dots expanding the range of optical, electronic, and magnetic functionality.

Vegetation correlates of gibbon density in the peat-swamp forest of the Sabangau catchment, Central Kalimantan, Indonesia.

PubMed

Hamard, Marie; Cheyne, Susan M; Nijman, Vincent

2010-06-01

Understanding the complex relationship between primates and their habitats is essential for effective conservation plans. Peat-swamp forest has recently been recognized as an important habitat for the Southern Bornean gibbon (Hylobates albibarbis), but information is scarce on the factors that link gibbon density to characteristics of this unique ecosystem. Our aims in this study were firstly to estimate gibbon density in different forest subtypes in a newly protected, secondary peat-swamp forest in the Sabangau Catchment, Indonesia, and secondly to identify which vegetation characteristics correlate with gibbon density. Data collection was conducted in a 37.1 km(2) area, using auditory sampling methods and vegetation "speed plotting". Gibbon densities varied between survey sites from 1.39 to 3.92 groups/km(2). Canopy cover, tree height, density of large trees and food availability were significantly correlated with gibbon density, identifying the preservation of tall trees and good canopy cover as a conservation priority for the gibbon population in the Sabangau forest. This survey indicates that selective logging, which specifically targets large trees and disrupts canopy cover, is likely to have adverse effects on gibbon populations in peat-swamp forests, and calls for greater protection of these little-studied ecosystems. (c) 2010 Wiley-Liss, Inc.

Unique attributes of cyanobacterial metabolism revealed by improved genome-scale metabolic modeling and essential gene analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Broddrick, Jared T.; Rubin, Benjamin E.; Welkie, David G.

The model cyanobacterium, Synechococcus elongatus PCC 7942, is a genetically tractable obligate phototroph that is being developed for the bioproduction of high-value chemicals. Genome-scale models (GEMs) have been successfully used to assess and engineer cellular metabolism; however, GEMs of phototrophic metabolism have been limited by the lack of experimental datasets for model validation and the challenges of incorporating photon uptake. In this paper, we develop a GEM of metabolism in S. elongatus using random barcode transposon site sequencing (RB-TnSeq) essential gene and physiological data specific to photoautotrophic metabolism. The model explicitly describes photon absorption and accounts for shading, resulting inmore » the characteristic linear growth curve of photoautotrophs. GEM predictions of gene essentiality were compared with data obtained from recent dense-transposon mutagenesis experiments. This dataset allowed major improvements to the accuracy of the model. Furthermore, discrepancies between GEM predictions and the in vivo dataset revealed biological characteristics, such as the importance of a truncated, linear TCA pathway, low flux toward amino acid synthesis from photorespiration, and knowledge gaps within nucleotide metabolism. Finally, coupling of strong experimental support and photoautotrophic modeling methods thus resulted in a highly accurate model of S. elongatus metabolism that highlights previously unknown areas of S. elongatus biology.« less

The HMX/NKX homeodomain protein MLS-2 specifies the identity of the AWC sensory neuron type via regulation of the ceh-36 Otx gene in C. elegans

PubMed Central

Kim, Kyuhyung; Kim, Rinho; Sengupta, Piali

2010-01-01

The differentiated features of postmitotic neurons are dictated by the expression of specific transcription factors. The mechanisms by which the precise spatiotemporal expression patterns of these factors are regulated are poorly understood. In C. elegans, the ceh-36 Otx homeobox gene is expressed in the AWC sensory neurons throughout postembryonic development, and regulates terminal differentiation of this neuronal subtype. Here, we show that the HMX/NKX homeodomain protein MLS-2 regulates ceh-36 expression specifically in the AWC neurons. Consequently, the AWC neurons fail to express neuron type-specific characteristics in mls-2 mutants. mls-2 is expressed transiently in postmitotic AWC neurons, and directly initiates ceh-36 expression. CEH-36 subsequently interacts with a distinct site in its cis-regulatory sequences to maintain its own expression, and also directly regulates the expression of AWC-specific terminal differentiation genes. We also show that MLS-2 acts in additional neuron types to regulate their development and differentiation. Our analysis describes a transcription factor cascade that defines the unique postmitotic characteristics of a sensory neuron subtype, and provides insights into the spatiotemporal regulatory mechanisms that generate functional diversity in the sensory nervous system. PMID:20150279

Characteristics of sites and trees affected by rapid white oak mortality as reported by forestry professionals in Missouri

Treesearch

Sharon E. Reed; James T. English; Rose-Marie Muzika; John M. Kabrick; Simeon. Wright

2017-01-01

A new syndrome was named rapid white oak mortality in 2011 to describe the rapid death of white oak trees (Quercus alba L.) within one growing season. A survey with 24 questions about stand and site characteristics, site history, and symptoms was distributed to forestry professionals to gather information about the new syndrome. Sixty-three reports...

Human embryonic stem cell phosphoproteome revealed by electron transfer dissociation tandem mass spectrometry

PubMed Central

Swaney, Danielle L.; Wenger, Craig D.; Thomson, James A.; Coon, Joshua J.

2009-01-01

Protein phosphorylation is central to the understanding of cellular signaling, and cellular signaling is suggested to play a major role in the regulation of human embryonic stem (ES) cell pluripotency. Here, we describe the use of conventional tandem mass spectrometry-based sequencing technology—collision-activated dissociation (CAD)—and the more recently developed method electron transfer dissociation (ETD) to characterize the human ES cell phosphoproteome. In total, these experiments resulted in the identification of 11,995 unique phosphopeptides, corresponding to 10,844 nonredundant phosphorylation sites, at a 1% false discovery rate (FDR). Among these phosphorylation sites are 5 localized to 2 pluripotency critical transcription factors—OCT4 and SOX2. From these experiments, we conclude that ETD identifies a larger number of unique phosphopeptides than CAD (8,087 to 3,868), more frequently localizes the phosphorylation site to a specific residue (49.8% compared with 29.6%), and sequences whole classes of phosphopeptides previously unobserved. PMID:19144917

Review and Analysis of Algorithmic Approaches Developed for Prognostics on CMAPSS Dataset

DTIC Science & Technology

2014-12-23

publications for benchmarking prognostics algorithms. The turbofan degradation datasets have received over seven thousand unique downloads in the last five...approaches that researchers have taken to implement prognostics using these turbofan datasets. Some unique characteristics of these datasets are also...Description of the five turbofan degradation datasets available from NASA repository. Datasets #Fault Modes #Conditions #Train Units #Test Units

The unique radar scattering properties of silicic lava flows and domes

NASA Technical Reports Server (NTRS)

Plaut, Jeffrey J.; Stofan, Ellen R.; Anderson, Steven W.; Crown, David A.

1995-01-01

Silicic (silica-rich) lava flows, such as rhyolite, rhyodacite, and dacite, possess unique physical properties primarily because of the relatively high viscosity of the molten lava. Silicic flows tend to be thicker than basaltic flows, and the resulting large-scale morphology is typically a steep-sided dome or flow lobe, with aspect ratios (height/length) sometimes approaching unity. The upper surfaces of silicic domes and flows are normally emplaced as relatively cool, brittle slabs that fracture as they are extruded from the central vent areas, and are then rafted away toward the flow margin as a brittle carapace above a more ductile interior layer. This mode of emplacement results in a surface with unique roughness characteristics, which can be well-characterized by multiparameter synthetic aperture radar (SAR) observations. In this paper, we examine the scattering properties of several silicic domes in the Inyo volcanic chain in the Eastern Sierra of California, using AIRSAR and TOPSAR data. Field measurements of intermediate-scale (cm to tens of m) surface topography and block size are used to assess the mechanisms of the scattering process, and to quantify the unique roughness characteristics of the flow surfaces.

Preparing Generation Z for the Teaching Profession

ERIC Educational Resources Information Center

Carter, Tim

2018-01-01

Generation Z, also known as the Homeland Generation, is the most recent generational cohort to enter the university setting. As with other generational cohorts, various shaping factors have impacted this group contributing to its unique and defining characteristics. When carefully considered, these characteristics may provide insight into how to…

Students with Emotional Disturbances: How Can School Counselors Serve?

ERIC Educational Resources Information Center

Miller, Lynne Guillot; Rainey, John S.

2008-01-01

Students with Emotional Disturbances (ED) possess unique characteristics that require additional care from school counselors, teachers, and other school personnel. Information pertaining to the prevalence of ED among students and the common characteristics of students with ED is reviewed. Additionally, ideas and effective approaches that will aid…

Maternal Sensitivity and Child Responsiveness: Associations with Social Context, Maternal Characteristics, and Child Characteristics in a Multivariate Analysis

ERIC Educational Resources Information Center

Bornstein, Marc H.; Hendricks, Charlene; Haynes, O. Maurice; Painter, Kathleen M.

2007-01-01

This study examined unique associations of multiple distal context variables (family socioeconomic status [SES], maternal employment, and paternal parenting) and proximal maternal (personality, intelligence, and knowledge; behavior, self-perceptions, and attributions) and child (age, gender, representation, language, and sociability)…

Some Current Findings on Brain Characteristics of the Mathematically Gifted Adolescent

ERIC Educational Resources Information Center

O'Boyle, Michael W.

2005-01-01

A number of studies investigating the brain characteristics of mathematically gifted youth indicate that they possess a unique functional organisation as compared to those of average math ability (O'Boyle, et al., 1995). Specifically, data from a variety of behavioural and psychophysiological experiments tend to suggest enhanced processing…

Biological characteristics and population status of steelhead (Oncorhynchus mykiss) in southeast Alaska.

Treesearch

Samuel C. Lohr; Mason D. Bryant

1999-01-01

We reviewed existing data to determine the range and distribution of steelhead (Oncorhynchus mykiss) in southeast Alaska, summarized biological characteristics, and determined population status of steelhead stocks. Unique or sensitive stocks that may require consideration in planning land management activities are identified within the data...

Malaysian Gifted Students' Use of English Language Learning Strategies

ERIC Educational Resources Information Center

Yunus, Melor Md; Sulaiman, Nur Ainil; Embi, Mohammed Amin

2013-01-01

Many studies have been done on language learning strategies employed by different type of learners and in various contexts. However, very little studies have been done on gifted students regarding language learning. Gifted students have unique characteristics and have different ways of thinking and learning. These characteristics affect how they…

Trip generation rates, peaking characteristics, and vehicle mix characteristics of special West Virginia generators : Volume 2, appendices.

DOT National Transportation Integrated Search

2000-02-01

For a number of land uses, published trip rates were not appropriate for application in West Virginia. There are a number of so-called special genertators, which are either unique to West Virginia (i.e., regional jails) or have assumed increased impo...

Trip generation rates, peaking characteristics, and vehicle mix characteristics of special West Virginia generators : executive summary.

DOT National Transportation Integrated Search

2000-02-01

For a number of land uses, published trip rates were not appropriate for application in West Virginia. There are a number of so-called special generators, which are either unique to West Virginia (i.e., regional jails) or have assumed increased impor...

Internal Consistency and Associated Characteristics of Informant Discrepancies in Clinic Referred Youths Age 11 to 17 Years

PubMed Central

De Los Reyes, Andres; Youngstrom, Eric A.; Pabón, Shairy C.; Youngstrom, Jennifer K.; Feeny, Norah C.; Findling, Robert L.

2011-01-01

In this study, we examined the internal consistency of informant discrepancies in reports of youth behavior and emotional problems and their unique relations with youth, caregiver, and family characteristics. In a heterogeneous multisite clinic sample of 420 youths (ages 11 to 17 years), high internal consistency estimates were observed across measures of informant discrepancies. Further, latent profile analyses identified systematic patterns of discrepancies, characterized by their magnitude and direction (i.e., which informant reported greater youth problems). Additionally, informant discrepancies systematically and uniquely related to informants' own perspectives of youth mood problems, and these relations remained significant after taking into account multiple informants' reports of informant characteristics widely known to relate to informant discrepancies. These findings call into the question the prevailing view of informant discrepancies as indicative of unreliability and/or bias on the part of informants' reports of youths' behavior. PMID:21229442

Magma Differentiation Processes That Develop an "Enriched" Signature in the Izu Bonin Rear Arc: Evidence from Drilling at IODP Site U1437

NASA Astrophysics Data System (ADS)

Heywood, L. J.; DeBari, S. M.; Schindlbeck, J. C.; Escobar-Burciaga, R. D.

2015-12-01

The Izu Bonin rear arc represents a unique laboratory to study the development of continental crust precursors at an intraoceanic subduction zone., Volcanic output in the Izu Bonin rear arc is compositionally distinct from the Izu Bonin main volcanic front, with med- to high-K and LREE-enrichment similar to the average composition of the continental crust. Drilling at IODP Expedition 350 Site U1437 in the Izu Bonin rear arc obtained volcaniclastic material that was deposited from at least 13.5 Ma to present. IODP Expedition 350 represents the first drilling mission in the Izu Bonin rear arc region. This study presents fresh glass and mineral compositions (obtained via EMP and LA-ICP-MS) from unaltered tephra layers in mud/mudstone (Lithostratigraphic Unit I) and lapillistone (Lithostratigraphic Unit II) <4.5 Ma to examine the geochemical signature of Izu Bonin rear arc magmas. Unit II samples are coarse-grained tephras that are mainly rhyolitic in composition (72.1-77.5 wt. % SiO2, 3.2-3.9 wt. % K2O and average Mg# 24) and LREE-enriched. These rear-arc rhyolites have an average La/Sm of 2.6 with flat HREEs, average Th/La of 0.15, and Zr/Y of 4.86. Rear-arc rhyolite trace element signature is distinct from felsic eruptive products from the Izu Bonin main volcanic front, which have lower La/Sm and Th/La as well as significantly lower incompatible element concentrations. Rear arc rhyolites have similar trace element ratios to rhyolites from the adjacent but younger backarc knolls and actively-extending rift regions, but the latter is typified by lower K2O, as well as a smaller degree of enrichment in incompatible elements. Given these unique characteristics, we explore models for felsic magma formation and intracrustal differentiation in the Izu Bonin rear arc.

Nanoparticle drug-delivery systems for peritoneal cancers: a case study of the design, characterization and development of the expansile nanoparticle.

PubMed

Colby, Aaron H; Oberlies, Nicholas H; Pearce, Cedric J; Herrera, Victoria L M; Colson, Yolonda L; Grinstaff, Mark W

2017-05-01

Nanoparticle (NP)-based drug-delivery systems are frequently employed to improve the intravenous administration of chemotherapy; however, few reports explore their application as an intraperitoneal therapy. We developed a pH-responsive expansile nanoparticle (eNP) specifically designed to leverage the intraperitoneal route of administration to treat intraperitoneal malignancies, such as mesothelioma, ovarian, and pancreatic carcinomatoses. This review describes the design, evaluation, and evolution of the eNP technology and, specifically, a Materials-Based Targeting paradigm that is unique among the many active- and passive-targeting strategies currently employed by NP-delivery systems. pH-responsive eNP swelling is responsible for the extended residence at the target tumor site as well as the subsequent improvement in tumoral drug delivery and efficacy observed with paclitaxel-loaded eNPs (PTX-eNPs) compared to the standard clinical formulation of paclitaxel, Taxol®. Superior PTX-eNP efficacy is demonstrated in two different orthotopic models of peritoneal cancer-mesothelioma and ovarian cancer; in a third model-of pancreatic cancer-PTX-eNPs demonstrated comparable efficacy to Taxol with reduced toxicity. Furthermore, the unique structural and responsive characteristics of eNPs enable them to be used in three additional treatment paradigms, including: treatment of lymphatic metastases in breast cancer; use as a highly fluorescent probe to visually guide the resection of peritoneal implants; and, in a two-step delivery paradigm for concentrating separately administered NP and drug at a target site. This case study serves as an important example of using the targeted disease-state's pathophysiology to inform the NP design as well as the method of use of the delivery system. WIREs Nanomed Nanobiotechnol 2017, 9:e1451. doi: 10.1002/wnan.1451 For further resources related to this article, please visit the WIREs website. © 2017 Wiley Periodicals, Inc.

Biobanking in a Challenging African Environment: Unique Experience from the SIREN Project.

PubMed

Akinyemi, Rufus O; Akinwande, Kazeem; Diala, Samuel; Adeleye, Osi; Ajose, Abiodun; Issa, Kehinde; Owusu, Dorcas; Boamah, Isaac; Yahaya, Isah Suleiman; Jimoh, Abdulraheem Olayemi; Imoh, Lucius; Fakunle, Gregory; Akpalu, Albert; Sarfo, Fred; Wahab, Kolawole; Sanya, Emmanuel; Owolabi, Lukman; Obiako, Reginald; Osaigbovo, Godwin; Komolafe, Morenikeji; Fawale, Michael; Adebayo, Philip; Olowoyo, Paul; Obiabo, Yahaya; Sunmonu, Taofiki; Chukwuonye, Ijezie; Balogun, Olayemi; Adeoye, Basirat; Oladele, Florence; Olowoniyi, Peter; Adeyemi, Frederick; Lezzi, Arthur; Falayi, Ajibola Tunde; Fasanya, Michael; Ogunwale, Kolawole; Adeola, Olabisi; Olomu, Omolara; Aridegbe, Olumayowa; Laryea, Ruth; Uvere, Ezinne; Faniyan, Moyinoluwalogo; Melikam, Ezinne; Tagge, Raelle; Akpa, Onoja; Akinyemi, Joshua; Arulogun, Oyedunni; Tiwari, Hemant K; Ovbiagele, Bruce; Owolabi, Mayowa

2018-05-07

Africa was previously insufficiently represented in the emerging discipline of biobanking despite commendable early efforts. However, with the Human, Heredity, and Health in Africa (H3Africa) initiative, biorepository science has been bolstered, regional biobanks are springing up, and awareness about biobanks is growing on the continent. The Stroke Investigative Research and Educational Network (SIREN) project is a transnational, multicenter, hospital and community-based study involving over 3000 cases and 3000 controls recruited from 16 sites in Ghana and Nigeria. SIREN aims to explore and unravel the genetic and environmental factors that interact to produce the peculiar phenotypic and clinical characteristics of stroke as seen in people of African ancestry and facilitate the development of new diagnostics, therapeutics, and preventative strategies. The aim of this article is to describe our experience with the development of the procedure for collection, processing, storage, and shipment of biological samples (blood, serum, plasma, buffy coat, red cell concentrates, and DNA) and brain imaging across coordinating and participating sites within the SIREN Project. The SIREN network was initiated in 2014 with support and funding from the H3Africa Initiative. The SIREN Biobank currently has 3015 brain images, 92,950 blood fractions (serum, plasma, red cell concentrates, and buffy coat) accrued from 8450 recruited subjects, and quantified and aliquoted good-quality DNA extracts from 6150 study subjects. This represents an invaluable resource for future research with expanding genomic and trans-omic technologies. This will facilitate the involvement of indigenous African samples in cutting-edge stroke genomics and trans-omics research. It is, however, critical to effectively engage African stroke patients and community members who have contributed precious biological materials to the SIREN Biobank to generate appropriate evidence base for dealing with ethical, legal, and social issues of privacy, autonomy, identifiability, biorights, governance issues, and public understanding of stroke biobanking in the context of unique African culture, language, and belief systems.

Unique Construction and Social Experiences in Residential Remediation Sites - 13423

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jung, Paul; Scarborough, Rebecca

2013-07-01

Sevenson Environmental Services, Inc., (Sevenson) has performed several radiological remediation projects located in residential urban areas. Over the course of these projects, there has been a wide variety of experiences encountered from construction related issues to unique social situations. Some of the construction related issues included the remediation of interior basements where contaminated material was located under the footers of the structure or was used in the mortar between cinder block or field stone foundations. Other issues included site security, maintaining furnaces or other utilities, underpinning, backfilling and restoration. In addition to the radiological hazards associated with this work theremore » were occupational safety and industrial hygiene issues that had to be addressed to ensure the safety and health of neighboring properties and residents. The unique social situations at these job sites have included arson, theft/stolen property, assault/battery, prostitution, execution of arrest warrants for residents, discovery of drugs and paraphernalia, blood borne pathogens, and unexploded ordnance. Some of these situations have become a sort of comical urban legend throughout the organization. One situation had historical significance, involving the demolition of a house to save a tree older than the Declaration of Independence. All of these projects typically involve the excavation of early 20. century items such as advertisement signs, various old bottles (milk, Listerine, perfume, whisky) and other miscellaneous common trash items. (authors)« less

Construction of New Campylobacter Cloning Vectors and a New Mutational Cat Cassette

DTIC Science & Technology

1993-01-01

mutational cat cassette PE - 61102A PR - 3M161102 6. AUTHOR(S) TA - BS13AK Yao R, Aim RA, Trust TJ, Guerry P WU- 1291 7. PERFORMING ORGANIZATION NAME(S) AND...mutational cat cassette %~ccesion For (Site-specific mutagenesis; recombinant DNA; multiple cloning site; PCR; shuttle vectors) NTIS CRA&I OTIC TAB E...campylobacter portion of these vectors, only three CAT , Cm acetyllraaseriase; car, gene encoding CAT , Cm, restriction sites in the IacZ MCS remain unique

IMS Infrasound Monitoring Test Site at Trafelberg, Austria: Preliminary Results from In-Situ Response Measurements of Infrasound Elements

DTIC Science & Technology

2010-09-01

IMS INFRASOUND MONITORING TEST SITE AT TRAFELBERG, AUSTRIA: PRELIMINARY RESULTS FROM IN-SITU RESPONSE MEASUREMENTS OF INFRASOUND ELEMENTS Thomas...The International Monitoring System (IMS) of the Comprehensive Nuclear Test-Ban Treaty Organization (CTBTO) has a unique infrasound test site...all four infrasound elements at I99 were made from 0.008 Hz to several Hz. For all four elements, from 0.01 to 0.1 Hz, the measured magnitude and

Riparian and Associated Habitat Characteristics Related to Nutrient Concentrations and Biological Responses of Small Streams in Selected Agricultural Areas, United States, 2003-04

USGS Publications Warehouse

Zelt, Ronald B.; Munn, Mark D.

2009-01-01

Physical factors, including both in-stream and riparian habitat characteristics that limit biomass or otherwise regulate aquatic biological condition, have been identified by previous studies. However, linking the ecological significance of nutrient enrichment to habitat or landscape factors that could allow for improved management of streams has proved to be a challenge in many regions, including agricultural landscapes, where many ecological stressors are strong and the variability among watersheds typically is large. Riparian and associated habitat characteristics were sampled once during 2003-04 for an intensive ecological and nutrients study of small perennial streams in five contrasting agricultural landscapes across the United States to determine how biological communities and ecosystem processes respond to varying levels of nutrient enrichment. Nutrient concentrations were determined in stream water at two different sampling times per site and biological samples were collected once per site near the time of habitat characterization. Data for 141 sampling sites were compiled, representing five study areas, located in parts of the Delmarva Peninsula (Delaware and Maryland), Georgia, Indiana, Ohio, Nebraska, and Washington. This report examines the available data for riparian and associated habitat characteristics to address questions related to study-unit contrasts, spatial scale-related differences, multivariate correlation structure, and bivariate relations between selected habitat characteristics and either stream nutrient conditions or biological responses. Riparian and associated habitat characteristics were summarized and categorized into 22 groups of habitat variables, with 11 groups representing land-use and land-cover characteristics and 11 groups representing other riparian or in-stream habitat characteristics. Principal components analysis was used to identify a reduced set of habitat variables that describe most of the variability among the sampled sites. The habitat characteristics sampled within the five study units were compared statistically. Bivariate correlations between riparian habitat variables and either nutrient-chemistry or biological-response variables were examined for all sites combined, and for sites within each study area. Nutrient concentrations were correlated with the extent of riparian cropland. For nitrogen species, these correlations were more frequently at the basin scale, whereas for phosphorus, they were about equally frequent at the segment and basin scales. Basin-level extents of riparian cropland and reach-level bank vegetative cover were correlated strongly with both total nitrogen and dissolved inorganic nitrogen (DIN) among multiple study areas, reflecting the importance of agricultural land-management and conservation practices for reducing nitrogen delivery from near-stream sources. When sites lacking segment-level wetlands were excluded, the negative correlation of riparian wetland extent with DIN among 49 sites was strong at the reach and segment levels. Riparian wetland vegetation thus may be removing dissolved nutrients from soil water and shallow groundwater passing through riparian zones. Other habitat variables that correlated strongly with nitrogen and phosphorus species included suspended sediment, light availability, and antecedent water temperature. Chlorophyll concentrations in seston were positively correlated with phosphorus concentrations for all sites combined. Benthic chlorophyll was correlated strongly with nutrient concentrations in only the Delmarva study area and only in fine-grained habitats. Current velocity or hydraulic scour could explain correlation patterns for benthic chlorophyll among Georgia sites, whereas chlorophyll in seston was correlated with antecedent water temperature among Washington and Delmarva sites. The lack of any consistent correlation pattern between habitat characteristics and organic material density (ash-free dry mass)

"That Dog Won't Hunt!": Exemplary School Change Efforts within the Kentucky Reform.

ERIC Educational Resources Information Center

Wolf, Shelby A.; Borko, Hilda; Elliott, Rebekah L.; McIver, Monette C.

2000-01-01

Presents case studies of four exemplary schools as they worked to meet the demands of the Kentucky Education Reform Act (KERA) and the assessment system, the Kentucky Instructional Results Information System. Finds that the ways schools met the challenges were unique to each site, but outlines aspect common to teachers in all four sites. (SLD)

The use of Forest Service experimental forests and ranges for long-term research on invasive species

Treesearch

Ralph Holiday Crawford; Gary W. Miller

2010-01-01

The 81 experimental forests and ranges (EFRs) research sites make the U.S. Department of Agriculture (USDA), Forest Service unique among land management agencies. The EFRs were established for conducting applied research that serves as a basis for managing forests and rangelands. Most EFR research sites have long histories of experimentation and research that provide...

Health Care Insights: A Unique Introduction to the Health Care Industry. [Fourth Edition]. Career Exploration.

ERIC Educational Resources Information Center

GMS Partners, Inc. Silver Spring, MD.

The Insights program begins the journey of becoming ready to work by introducing students to the varied career opportunities, skills, and technologies in today's health care industry. Students travel as a class or group to the host site for a series of seminars. While on site, the students and their accompanying teachers meet with professionals…

Effectiveness of seining after electrofishing to characterize stream fish communities

USGS Publications Warehouse

Meador, Michael R.

2012-01-01

The richness and composition of species collected uniquely to electrofishing and subsequent seining efforts were examined at 271 stream sites across the USA by using wadeable electrofishing methods (backpack or barge electrofishing) or boat electrofishing followed by seining. Seining after wadeable electrofishing resulted in the collection of new species at 42% of sites, whereas seining after boat electrofishing resulted in the collection of new species at 87% of sites. Mean percentage of total observed fish species richness that was collected uniquely by seining was 6% (representing one new species, on average) after wadeable electrofishing compared with 18% (four new species, on average) after boat electrofishing. Shannon–Wiener diversity index values were not significantly different between data from combined sampling (electrofishing and seining) and data from wadeable electrofishing (P = 0.490) but were significantly different between boat electrofishing and combined sampling (P = 0.004). Seining efforts after boat electrofishing can provide critical information that allows for a more complete characterization of the fish community. For sampling in wadeable streams, decisions regarding the use of seines should consider the effectiveness of electrofishing at a given site and the use of seines as an alternative primary sampling gear rather than as a supplement to electrofishing.

Unique Associations between Childhood Temperament Characteristics and Subsequent Psychopathology Symptom Trajectories from Childhood to Early Adolescence.

PubMed

Forbes, Miriam K; Rapee, Ronald M; Camberis, Anna-Lisa; McMahon, Catherine A

2017-08-01

Existing research suggests that temperamental traits that emerge early in childhood may have utility for early detection and intervention for common mental disorders. The present study examined the unique relationships between the temperament characteristics of reactivity, approach-sociability, and persistence in early childhood and subsequent symptom trajectories of psychopathology (depression, anxiety, conduct disorder, and attention-deficit hyperactivity disorder; ADHD) from childhood to early adolescence. Data were from the first five waves of the older cohort from the Longitudinal Study of Australian Children (n = 4983; 51.2% male), which spanned ages 4-5 to 12-13. Multivariate ordinal and logistic regressions examined whether parent-reported child temperament characteristics at age 4-5 predicted the study child's subsequent symptom trajectories for each domain of psychopathology (derived using latent class growth analyses), after controlling for other presenting symptoms. Temperament characteristics differentially predicted the symptom trajectories for depression, anxiety, conduct disorder, and ADHD: Higher levels of reactivity uniquely predicted higher symptom trajectories for all 4 domains; higher levels of approach-sociability predicted higher trajectories of conduct disorder and ADHD, but lower trajectories of anxiety; and higher levels of persistence were related to lower trajectories of conduct disorder and ADHD. These findings suggest that temperament is an early identifiable risk factor for the development of psychopathology, and that identification and timely interventions for children with highly reactive temperaments in particular could prevent later mental health problems.

Orchestration of Structural, Stereoelectronic, and Hydrogen-Bonding Effects in Stabilizing Triplexes from Engineered Chimeric Collagen Peptides (Pro(X)-Pro(Y)-Gly)6 Incorporating 4(R/S)-Aminoproline.

PubMed

Umashankara, Muddegowda; Sonar, Mahesh V; Bansode, Nitin D; Ganesh, Krishna N

2015-09-04

Collagens are an important family of structural proteins found in the extracellular matrix with triple helix as the characteristic structural motif. The collagen triplex is made of three left-handed polyproline II (PPII) helices with each PPII strand consisting of repetitive units of the tripeptide motif X-Y-Gly, where the amino acids X and Y are most commonly proline (Pro) and 4R-hydroxyproline (Hyp), respectively. A C4-endo pucker at X-site and C4-exo pucker at Y-site have been proposed to be the key for formation of triplex, and the nature of pucker is dependent on both the electronegativity and stereochemistry of the substituent. The present manuscript describes a new class of collagen analogues-chimeric cationic collagens-wherein both X- and Y-sites in collagen triad are simultaneously substituted by a combination of 4(R/S)-(OH/NH2/NH3(+)/NHCHO)-prolyl units and triplex stabilities measured at different pHs and in EG:H2O. Based on the results a model has been proposed with the premise that any factors which specifically favor the ring puckers of C4-endo at X-site and C4-exo at Y-site stabilize the PPII conformation and hence the derived triplexes. The pH-dependent triplex stability uniquely observed with ionizable 4-amino substituent on proline enables one to define the critical combination of factors C4-(exo/endo), intraresidue H-bonding, stereoelectronic (R/S) and n → π* interactions in dictating the triplex strength. The ionizable NH2 substituent at C4 in R/S configuration is thus a versatile probe for delineating the triplex stabilizing factors and the results have potential for designing of collagen analogues with customized properties for material and biological applications.

Soil characteristics of semidesert soils along a precipitation gradient in the Negev (Israel)

NASA Astrophysics Data System (ADS)

Steckenmesser, Daniel; Drahorad, Sylvie; Felix-Henningsen, Peter

2010-05-01

The sand dunes of the north-western Negev desert (Israel) show a unique precipitation gradient on a short distance. This area is build up by the same parent material and suited to investigate the influence of changes in rainfall on soil characteristics in semi-desert ecosystems. The study site is the western extension of the Sinai sand field, the sand dunes are stabilised by biological soil crusts and perennial vegetation like Retama raetam. Along this precipitation gradient the three investigation areas Nizzana South (90mm ^a-), Nizzana 84 (130mm ^a-1) and Nizzana 69 (170mm ^a-1) are situated. At every study site two soil profiles were investigated, each under the legume Retama raetam and in the bare interspace covered by biological soil crusts. The soil samples were taken at the interdune positions at every study site. The soil sampling included the biological soil crust, the topsoil and the subsoil up to 1,5 m. The narrow sampling of 20cm wide steps allow a mapping of the distribution of nutrients, carbonates and soluble salts of in order to show the impact of perennial plants and rainfall on soil properties. Soluble salts and nutrients were measured in a 1:5 water extraction, calcium carbonate was determined according to Scheibler. The data shows a strong influence of perennial shrubs on the deposition of dust and the redistribution of nutrients compared to the bare interspace. The distribution of highly and less soluble salts below the perennial shrub proofs a shallower water infiltration than in the comparable interspace area. The interspace between the plants is covered by a biological soil crust, which also strongly influences the matter fluxes by nutrient-fixation, creation of runoff and stabilization of the soil surface. These biological soil crusts show higher amounts of elements than the subsoils. The comparison of the three areas along the rainfall gradient shows higher inputs of soluble salts with increasing precipitation due to wet deposition, while carbonate contents are negatively correlated with decreasing precipitation. This is related to a higher dust input in the southern study site, which was generated in the lime stone Negev. Higher amounts of rainfall introduce higher element leaching. Perennial plants cover the surface and reduce infiltration. Inputs into the soils through dust have to be evaluated for every location to separate between effects of deposition and rainfall.

Composition, distribution, and potential toxicity of organochlorine mixtures in bed sediments of streams

USGS Publications Warehouse

Phillips, Patrick J.; Nowell, Lisa H.; Gilliom, Robert J.; Nakagaki, Naomi; Murray, Karen; VanAlstyne, Carolyn

2010-01-01

Mixtures of organochlorine compounds have the potential for additive or interactive toxicity to organisms exposed in the stream. This study uses a variety of methods to identify mixtures and a modified concentration-addition approach to estimate their potential toxicity at 845 stream sites across the United States sampled between 1992 and 2001 for organochlorine pesticides and polychlorinated biphenyls (PCBs) in bed sediment. Principal-component (PC) analysis identified five PCs that account for 77% of the total variance in 14 organochlorine compounds in the original dataset. The five PCs represent: (1) chlordane-related compounds and dieldrin; (2) p,p′-DDT and its degradates; (3) o,p′-DDT and its degradates; (4) the pesticide degradates oxychlordane and heptachlor epoxide; and (5) PCBs. The PC analysis grouped compounds that have similar chemical structure (such as parent compound and degradate), common origin (in the same technical pesticide mixture), and(or) similar relation of concentrations to land use. For example, the highest concentrations of chlordane compounds and dieldrin occurred at urban sites, reflecting past use of parent pesticides for termite control. Two approaches to characterizing mixtures—PC-based mixtures and unique mixtures—were applied to all 299 samples with a detection of two or more organochlorine compounds. PC-based mixtures are defined by the presence (in the sample) of one or more compounds associated with that PC. Unique mixtures are defined as a specific combination of two or more compounds detected in a sample, regardless of how many other compounds were also detected in that sample. The simplest PC-based mixtures (containing compounds from 1 or 2 PCs) commonly occurred in a variety of land use settings. Complex mixtures (containing compounds from 3 or more PCs) were most common in samples from urban and mixed/urban sites, especially in the Northeast, reflecting high concentrations of multiple chlordane, dieldrin, DDT-related compounds, and(or) PCBs. The most commonly occurring unique mixture (p,p′-DDE, p,p′-DDD) occurred in both simple and complex PC-based mixtures, and at both urban and agricultural sites. Mean Probable Effect Concentration Quotients (PEC-Q) values, which estimate the potential toxicity of organochlorine contaminant mixtures, were highest for complex mixtures. Mean PEC-Q values were highest for urban sites in the Northeast, followed by mixed/urban sites in the Northeast and agricultural sites in cotton growing areas. These results demonstrate that the PEC-Q approach can be used in combination with PC-based and unique mixture analyses to relate potential aquatic toxicity of contaminant mixtures to mixture complexity, land use, and other surrogates for contaminant sources.

Characteristics of aeroelastic instabilities in turbomachinery - NASA full scale engine test results

NASA Technical Reports Server (NTRS)

Lubomski, J. F.

1979-01-01

Several aeromechanical programs were conducted in the NASA/USAF Joint Engine System Research Programs. The scope of these programs, the instrumentation, data acquisition and reduction, and the test results are discussed. Data pertinent to four different instabilities were acquired; two types of stall flutter, choke flutter and a system mode instability. The data indicates that each instability has its own unique characteristics. These characteristics are described.

Temperature dependent characteristics of the random telegraph noise on contact resistive random access memory

NASA Astrophysics Data System (ADS)

Chang, Liang-Shun; Lin, Chrong Jung; King, Ya-Chin

2014-01-01

The temperature dependent characteristics of the random telegraphic noise (RTN) on contact resistive random access memory (CRRAM) are studied in this work. In addition to the bi-level switching, the occurrences of the middle states in the RTN signal are investigated. Based on the unique its temperature dependent characteristics, a new temperature sensing scheme is proposed for applications in ultra-low power sensor modules.

Physical, chemical, and biological data for two sites on the upper Kenai River, Alaska, 1998

USGS Publications Warehouse

Dorava, Joseph M.; Ness, Lee

1999-01-01

Water-quality data were collected and stream characteristics were documented from two sites along the upper Kenai River in the Kenai National Wildlife Refuge, Alaska. These data were collected to describe the current status of the sites and to provide baseline information from which changes in the future could be evaluated. Physical characteristics included channel geometry surveys, and measurements of channel widths and water discharge at each site. Chemical data included stream water temperature, dissolved-oxygen concentration, pH, specific conductance, E. coli and fecal coliform counts, and nutrient concentration. Data on concentrations of trace elements and various organic compounds in bed sediments and the tissue of slimy sculpin were also collected. Biological characteristics were evaluated using measurements of the bacteria, benthic macroinvertebrate, and fish communities.

Secrecy From Parents and Type 1 Diabetes Management in Late Adolescence

PubMed Central

Wiebe, Deborah J.; Van Bogart, Karina; Turner, Sara L.; Tucker, Christy; Butner, Jonathan E.; Berg, Cynthia A.

2015-01-01

Objectives This study examined (a) associations of parent–adolescent relationship characteristics and adolescent problem behavior with late adolescents’ secrecy from parents about type 1 diabetes management, and (b) whether secrecy was associated with diabetes and psychological outcomes independently of these factors. Methods Adolescents (N = 247, Mage = 17.76 years) completed survey measures of diabetes-related secrecy from parents, disclosure, parental acceptance, parental knowledge, and conduct problems. Mothers and adolescents reported on adolescent adherence to diabetes regimens and adolescents reported their depressive symptoms. Glycemic control was obtained from HbA1c test kits. Results Adolescent-reported disclosure to parents was uniquely negatively associated with secrecy from parents. Controlling for relationship variables, conduct problems, and sociodemographic and illness-related variables, secrecy from mothers was uniquely associated with poorer glycemic control and secrecy from both parents was associated with lower adherence. Conclusions Secrecy about type 1 diabetes management is uniquely associated with diabetes outcomes independent of other relationship characteristics and problem behaviors. PMID:26136405

Clinical-Physiological Considerations in Patients Undergoing Staged Palliation for a Functionally Single Ventricle.

PubMed

Penny, Daniel J; Krishnamurthy, Rajesh

2016-08-01

The objectives of this review are to discuss the pathophysiology of the circulation with a functionally univentricular heart, with a focus on the unique physiologic characteristics, which provide the underpinnings for the management of these complex patients. MEDLINE and PubMed. The circulation of the patient with a functionally univentricular heart displays unique physiologic characteristics, which are quite different from those of the normal biventricular circulation. There are profound differences within the heart itself in terms of ventricular function, interventricular interactions, and myocardial architecture, which are likely to have significant implications for the efficiency of ventricular ejection and metabolism. The coupling between the systemic ventricle and the aorta also displays unique features. The 3D orientation of the Fontan anastomosis itself can profoundly impact cardiac output, although the "portal" pulmonary arterial bed is a crucial determinant of overall cardiovascular function. As a result, disease-specific approaches to improve cardiovascular function are required at all stages during the care of these complex patients.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Duberstein, Corey A.; Simmons, Mary Ann; Sackschewsky, Michael R.

Mitigation threshold guidelines for the Hanford Site are based on habitat requirements of the sage sparrow (Amphispiza belli) and only apply to areas with a mature sagebrush (Artemisia tridentata) overstory and a native understory. The sage sparrow habitat requirements are based on literature values and are not specific to the Hanford Site. To refine these guidelines for the Site, a multi-year study was undertaken to quantify habitat characteristics of sage sparrow territories. These characteristics were then used to develop a habitat suitability index (HSI) model which can be used to estimate the habitat value of specific locations on the Site.

Human Performance Issues of Lunar-Sited Teleoperations

NASA Technical Reports Server (NTRS)

Kaiser, Mary K.; Null, Cynthia H. (Technical Monitor)

1995-01-01

Teleoperations in terrestrial environments present a number of challenges to system operators and designers. Transmission lags, restricted visual fields, and reduced or distorted tactile/kinesthetic feedback can compromise performance, especially for innerloop control tasks (e.g., vehicle or manipulator control). These problems are likely to be exacerbated in lunar operations, since teleoperation may occur across large distances. Further, the lunar environment will introduce unique concerns. For example, the teleoperated systems will reflect the reduced gravity of the moon. In addition to the novelty of these dynamics, operators will often have to cope with them while physically located in a terrestrial or microgravity environment. Similarly, the optical characteristics of the lunar environment differ from our usual experience (e.g., lack of atmospheric attenuation) in ways that may impact normative depth, distance, and motion perception. These human factors issues are related to the question of humans adapting to a lunar environment. However, teleoperations requires the operator to maintain functionality in both the control station and end-effector environments, defeating more straightforward environmental adaptation strategies.

Getting into the brain: liposome-based strategies for effective drug delivery across the blood–brain barrier

PubMed Central

Vieira, Débora B; Gamarra, Lionel F

2016-01-01

This review summarizes articles that have been reported in literature on liposome-based strategies for effective drug delivery across the blood–brain barrier. Due to their unique physicochemical characteristics, liposomes have been widely investigated for their application in drug delivery and in vivo bioimaging for the treatment and/or diagnosis of neurological diseases, such as Alzheimer’s, Parkinson’s, stroke, and glioma. Several strategies have been used to deliver drug and/or imaging agents to the brain. Covalent ligation of such macromolecules as peptides, antibodies, and RNA aptamers is an effective method for receptor-targeting liposomes, which allows their blood–brain barrier penetration and/or the delivery of their therapeutic molecule specifically to the disease site. Additionally, methods have been employed for the development of liposomes that can respond to external stimuli. It can be concluded that the development of liposomes for brain delivery is still in its infancy, although these systems have the potential to revolutionize the ways in which medicine is administered. PMID:27799765

Plastid, nuclear and reverse transcriptase sequences in the mitochondrial genome of Oenothera: is genetic information transferred between organelles via RNA?

PubMed Central

Schuster, W; Brennicke, A

1987-01-01

We describe an open reading frame (ORF) with high homology to reverse transcriptase in the mitochondrial genome of Oenothera. This ORF displays all the characteristics of an active plant mitochondrial gene with a possible ribosome binding site and 39% T in the third codon position. It is located between a sequence fragment from the plastid genome and one of nuclear origin downstream from the gene encoding subunit 5 of the NADH dehydrogenase. The nuclear derived sequence consists of 528 nucleotides from the small ribosomal RNA and contains an expansion segment unique to nuclear rRNAs. The plastid sequence contains part of the ribosomal protein S4 and the complete tRNA(Ser). The observation that only transcribed sequences have been found i more than one subcellular compartment in higher plants suggests that interorganellar transfer of genetic information may occur via RNA and subsequent local reverse transcription and genomic integration. PMID:14650433