33 CFR 117.633 - Grand River.

Code of Federal Regulations, 2014 CFR

2014-07-01

... March 16 through December 14, from 6:30 a.m. to 8:30 p.m., seven days a week, once an hour, on the half-hour; except the draw need not open for pleasure craft at 7:30 a.m., 12:30 p.m., and 5:30 p.m. on... be passed through the draw of each bridge as soon as possible. (b) The draw of the CSX Transportation...

33 CFR 117.633 - Grand River.

Code of Federal Regulations, 2013 CFR

2013-07-01

... March 16 through December 14, from 6:30 a.m. to 8:30 p.m., seven days a week, once an hour, on the half-hour; except the draw need not open for pleasure craft at 7:30 a.m., 12:30 p.m., and 5:30 p.m. on... be passed through the draw of each bridge as soon as possible. (b) The draw of the CSX Transportation...

33 CFR 117.633 - Grand River.

Code of Federal Regulations, 2011 CFR

2011-07-01

... March 16 through December 14, from 6:30 a.m. to 8:30 p.m., seven days a week, once an hour, on the half-hour; except the draw need not open for pleasure craft at 7:30 a.m., 12:30 p.m., and 5:30 p.m. on... be passed through the draw of each bridge as soon as possible. (b) The draw of the CSX Transportation...

33 CFR 117.833 - Pasquotank River.

Code of Federal Regulations, 2013 CFR

2013-07-01

... City, shall open on signal; except that between 7 a.m. and 9 a.m., and 4 p.m. and 6 p.m., Monday through Friday, the draw need open only at 7:30 a.m., 8:30 a.m., 4:30 p.m., and 5:30 p.m. for any pleasure vessels waiting to pass. [CGD05-94-118, 60 FR 40098, Aug. 7, 1995, as amended by CGD05-97-009, 62 FR 66006...

33 CFR 117.833 - Pasquotank River.

Code of Federal Regulations, 2014 CFR

2014-07-01

... City, shall open on signal; except that between 7 a.m. and 9 a.m., and 4 p.m. and 6 p.m., Monday through Friday, the draw need open only at 7:30 a.m., 8:30 a.m., 4:30 p.m., and 5:30 p.m. for any pleasure vessels waiting to pass. [CGD05-94-118, 60 FR 40098, Aug. 7, 1995, as amended by CGD05-97-009, 62 FR 66006...

33 CFR 117.833 - Pasquotank River.

Code of Federal Regulations, 2012 CFR

2012-07-01

... City, shall open on signal; except that between 7 a.m. and 9 a.m., and 4 p.m. and 6 p.m., Monday through Friday, the draw need open only at 7:30 a.m., 8:30 a.m., 4:30 p.m., and 5:30 p.m. for any pleasure vessels waiting to pass. [CGD05-94-118, 60 FR 40098, Aug. 7, 1995, as amended by CGD05-97-009, 62 FR 66006...

33 CFR 117.833 - Pasquotank River.

Code of Federal Regulations, 2011 CFR

2011-07-01

... City, shall open on signal; except that between 7 a.m. and 9 a.m., and 4 p.m. and 6 p.m., Monday through Friday, the draw need open only at 7:30 a.m., 8:30 a.m., 4:30 p.m., and 5:30 p.m. for any pleasure vessels waiting to pass. [CGD05-94-118, 60 FR 40098, Aug. 7, 1995, as amended by CGD05-97-009, 62 FR 66006...

rBCG30-Induced Immunity and Cross-Protection against Mycobacterium leprae Challenge Are Enhanced by Boosting with the Mycobacterium tuberculosis 30-Kilodalton Antigen 85B

PubMed Central

Gillis, Thomas P.; Tullius, Michael V.

2014-01-01

Leprosy remains a major global health problem and typically occurs in regions in which tuberculosis is endemic. Vaccines are needed that protect against both infections and do so better than the suboptimal Mycobacterium bovis BCG vaccine. Here, we evaluated rBCG30, a vaccine previously demonstrated to induce protection superior to that of BCG against Mycobacterium tuberculosis and Mycobacterium bovis challenge in animal models, for efficacy against Mycobacterium leprae challenge in a murine model of leprosy. rBCG30 overexpresses the M. tuberculosis 30-kDa major secretory protein antigen 85B, which is 85% homologous with the M. leprae homolog (r30ML). Mice were sham immunized or immunized intradermally with BCG or rBCG30 and challenged 2.5 months later by injection of viable M. leprae into each hind footpad. After 7 months, vaccine efficacy was assessed by enumerating the M. leprae bacteria per footpad. Both BCG and rBCG30 induced significant protection against M. leprae challenge. In the one experiment in which a comparison between BCG and rBCG30 was feasible, rBCG30 induced significantly greater protection than did BCG. Immunization of mice with purified M. tuberculosis or M. leprae antigen 85B also induced protection against M. leprae challenge but less so than BCG or rBCG30. Notably, boosting rBCG30 with M. tuberculosis antigen 85B significantly enhanced r30ML-specific immune responses, substantially more so than boosting BCG, and significantly augmented protection against M. leprae challenge. Thus, rBCG30, a vaccine that induces improved protection against M. tuberculosis, induces cross-protection against M. leprae that is comparable or potentially superior to that induced by BCG, and boosting rBCG30 with antigen 85B further enhances immune responses and protective efficacy. PMID:25001602

rBCG30-induced immunity and cross-protection against Mycobacterium leprae challenge are enhanced by boosting with the Mycobacterium tuberculosis 30-kilodalton antigen 85B.

PubMed

Gillis, Thomas P; Tullius, Michael V; Horwitz, Marcus A

2014-09-01

Leprosy remains a major global health problem and typically occurs in regions in which tuberculosis is endemic. Vaccines are needed that protect against both infections and do so better than the suboptimal Mycobacterium bovis BCG vaccine. Here, we evaluated rBCG30, a vaccine previously demonstrated to induce protection superior to that of BCG against Mycobacterium tuberculosis and Mycobacterium bovis challenge in animal models, for efficacy against Mycobacterium leprae challenge in a murine model of leprosy. rBCG30 overexpresses the M. tuberculosis 30-kDa major secretory protein antigen 85B, which is 85% homologous with the M. leprae homolog (r30ML). Mice were sham immunized or immunized intradermally with BCG or rBCG30 and challenged 2.5 months later by injection of viable M. leprae into each hind footpad. After 7 months, vaccine efficacy was assessed by enumerating the M. leprae bacteria per footpad. Both BCG and rBCG30 induced significant protection against M. leprae challenge. In the one experiment in which a comparison between BCG and rBCG30 was feasible, rBCG30 induced significantly greater protection than did BCG. Immunization of mice with purified M. tuberculosis or M. leprae antigen 85B also induced protection against M. leprae challenge but less so than BCG or rBCG30. Notably, boosting rBCG30 with M. tuberculosis antigen 85B significantly enhanced r30ML-specific immune responses, substantially more so than boosting BCG, and significantly augmented protection against M. leprae challenge. Thus, rBCG30, a vaccine that induces improved protection against M. tuberculosis, induces cross-protection against M. leprae that is comparable or potentially superior to that induced by BCG, and boosting rBCG30 with antigen 85B further enhances immune responses and protective efficacy. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Effects of Laparoscopic Roux-en-Y Gastric Bypass for Type 2 Diabetes Mellitus: Comparison of BMI > 30 and < 30 kg/m2.

PubMed

Ke, Zhigang; Li, Fan; Chen, Jing; Gao, Yu; Zhou, Xunmei; Sun, Fang; Li, Chunxue; Liu, Baohua; Li, Qiang; Zhu, Zhiming; Tong, Weidong

2017-11-01

Recently, many studies focused on type 2 diabetes mellitus (T2DM) patients with body mass index (BMI) < 30 kg/m 2 and suggested that those patients might benefit from Roux-en-Y gastric bypass (RYGB). However, evidence on its effectiveness to improve T2DM patients with BMI < 30 kg/m 2 is still lacking. The aim of this study is to explore whether T2DM patients with BMI < 30 kg/m 2 get similar surgical effect from RYGB compared with those patients with BMI > 30 kg/m 2 . Seventy patients with uncontrolled T2DM underwent laparoscopic RYGB from May 2010 to December 2015 in the GI Department of Daping Hospital. Weight, BMI, waist circumference, glucose, and lipid metabolic parameters were collected and evaluated at baseline and 1, 3, 6, 12, and 24 months postsurgery. Patients with BMI < 30 kg/m 2 were compared with those with BMI > 30 kg/m 2 . Among the 70 patients, 47 (67.1%) BMI < 30 kg/m 2 , and 23 (32.9%) BMI > 30 kg/m 2 . Patients with BMI < 30 kg/m 2 are significantly older; they are female predominant and have longer duration of diabetes. The complete remission of T2DM was 28.2% of the BMI < 30 kg/m 2 group and 57.9% of the BMI > 30 kg/m 2 group (p = 0.029). There was no significant difference in the change of glucose and lipid metabolic parameters of both groups. FPG, 2hPG, and HbA1c% levels were significantly improved after 1 month (p < 0.05), and then remained essentially stable from the sixth month in both groups. The 2-year study has shown that RYGB is a safe and effective procedure in treating T2DM with BMI < 30 kg/m 2 , although the complete remission of T2DM in the BMI < 30 kg/m 2 group is lower than the BMI > 30 kg/m 2 group.

Inventory of File naefs_geavg.t12z.ndgd_conusf30.grib2

Science.gov Websites

fcst Pressure [Pa] ens-mean 002 2 m above ground TMP 30 hour fcst Temperature [K] ens-mean 003 2 m above ground RH 30 hour fcst Relative Humidity [%] ens-mean 004 2 m above ground DPT 30 hour fcst Dew Point Temperature [K] ens-mean 005 10 m above ground UGRD 30 hour fcst U-Component of Wind [m/s] ens

Comparison of Allergenicity at Gly m 4 and Gly m Bd 30K of Soybean after Genetic Modification.

PubMed

Tsai, Jaw-Ji; Chang, Ching-Yun; Liao, En-Chih

2017-02-15

Despite rapid growth of genetically modified (GM) crops, effective evaluations of genetic modification on allergenicity are still lacking. Gly m Bd 30K is cross-reactive with cow's milk protein casein, Gly m 4, and with birch pollen allergen Bet v 1. Here we compared the allergenicity between GM and non-GM soybeans with respect to the foci Gly m 4 and Gly m Bd 30K. Recombinant allergens of Gly m Bd 30K and Gly m 4 were generated and polyclonal antibodies raised to identify these two allergenic components in soybeans. GM soybean was first PCR-confirmed using 35S promoter. A total of 20 soybeans (half GM, half non-GM) obtained from a food market were used to assess their allergenicity based on IgE-binding and histamine release. The concentrations of Gly m Bd 30K and Gly m 4 in soybeans were then determined. Most soybean-allergic patients (9 of 10) showed IgE-positive reactions to the allergen of 30 kDa in molecular weight. That allergen turned out to be Glycine max Gly m Bd 30K based on LC-MS/MS analyses. Gly m Bd 30K is therefore the major allergen in the soybean. An increase in the transcription of both the Gly m 4 (stress-induced protein SAM22) and Gly m Bd 28K (soybean allergen precursor) was found after genetic modification. The protein concentrations of Gly m 4 and Gly m Bd 30K were not statistically significant different between non-GM and GM soybeans. There were also no statistical significances between them in the tests of IgE binding and histamine release. In conclusion, soybeans showed similar concentrations of Gly m Bd 30K and Gly m 4 regardless of genetic modification or absence thereof. The allergenicity of both Gly m Bd 30K and Gly m 4 was therefore not altered after genetic modification. Patients showing hypersensitivity to soybeans and who had pre-existing allergy to birch pollen and cow's milk casein might not further increase their allergic reactions following exposures to the GM soybeans.

33 CFR 207.170c - Kissimmee River, navigation locks between Lake Tohopekaliga and Lake Okeechobee, Fla.; use...

Code of Federal Regulations, 2010 CFR

2010-07-01

....m. Saturday and Sunday Mar. 1 through Oct. 31 5:30 a.m. to 7:30 p.m. Do Nov. 1 through Feb. 28 5:30 a.m. to 6:30 p.m. Lock S-65A Seven days a week All year 8:00 a.m. to 5:00 p.m. Locks S-65B, S-65C, and S-65D Monday through Friday All year 8:00 a.m. to 5:00 p.m. Saturday and Sunday Mar. 1 through Oct...

33 CFR 207.170c - Kissimmee River, navigation locks between Lake Tohopekaliga and Lake Okeechobee, Fla.; use...

Code of Federal Regulations, 2013 CFR

2013-07-01

....m. Saturday and Sunday Mar. 1 through Oct. 31 5:30 a.m. to 7:30 p.m. Do Nov. 1 through Feb. 28 5:30 a.m. to 6:30 p.m. Lock S-65A Seven days a week All year 8:00 a.m. to 5:00 p.m. Locks S-65B, S-65C, and S-65D Monday through Friday All year 8:00 a.m. to 5:00 p.m. Saturday and Sunday Mar. 1 through Oct...

33 CFR 207.170c - Kissimmee River, navigation locks between Lake Tohopekaliga and Lake Okeechobee, Fla.; use...

Code of Federal Regulations, 2014 CFR

2014-07-01

....m. Saturday and Sunday Mar. 1 through Oct. 31 5:30 a.m. to 7:30 p.m. Do Nov. 1 through Feb. 28 5:30 a.m. to 6:30 p.m. Lock S-65A Seven days a week All year 8:00 a.m. to 5:00 p.m. Locks S-65B, S-65C, and S-65D Monday through Friday All year 8:00 a.m. to 5:00 p.m. Saturday and Sunday Mar. 1 through Oct...

33 CFR 207.170c - Kissimmee River, navigation locks between Lake Tohopekaliga and Lake Okeechobee, Fla.; use...

Code of Federal Regulations, 2012 CFR

2012-07-01

....m. Saturday and Sunday Mar. 1 through Oct. 31 5:30 a.m. to 7:30 p.m. Do Nov. 1 through Feb. 28 5:30 a.m. to 6:30 p.m. Lock S-65A Seven days a week All year 8:00 a.m. to 5:00 p.m. Locks S-65B, S-65C, and S-65D Monday through Friday All year 8:00 a.m. to 5:00 p.m. Saturday and Sunday Mar. 1 through Oct...

33 CFR 207.170c - Kissimmee River, navigation locks between Lake Tohopekaliga and Lake Okeechobee, Fla.; use...

Code of Federal Regulations, 2011 CFR

2011-07-01

....m. Saturday and Sunday Mar. 1 through Oct. 31 5:30 a.m. to 7:30 p.m. Do Nov. 1 through Feb. 28 5:30 a.m. to 6:30 p.m. Lock S-65A Seven days a week All year 8:00 a.m. to 5:00 p.m. Locks S-65B, S-65C, and S-65D Monday through Friday All year 8:00 a.m. to 5:00 p.m. Saturday and Sunday Mar. 1 through Oct...

75 FR 33506 - Safety Zones: Fireworks Displays in the Captain of the Port Portland Zone

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-14

... Festival Fireworks Display, Portland OR: May 28, 2010 from 8:30 p.m. until 10:30 p.m. (2) Cedco Inc...) Oregon Food Bank Blues Festival Fireworks, Portland, OR: July 4, 2010 from 8:30 p.m. through 11:30 p.m... Waterfront Festival, Aberdeen, WA: July 4, 2010 from 9 p.m. through 11 p.m. (10) Arlington Chamber of...

M30 expression demonstrates apoptotic cells, correlates with in situ end-labeling, and is associated with Ki-67 expression in large intestinal neoplasms.

PubMed

Carr, N J

2000-12-01

The monoclonal antibody M30 recognizes a neoepitope of cytokeratin 18 produced during apoptosis. It is reactive in formalin-fixed, paraffin-embedded tissue and has great potential in the study of apoptosis in clinical and experimental material. To compare the results of M30 immunoexpression with a more established technique of demonstrating apoptosis in tissue sections, in situ end-labeling. A secondary objective was to compare the results with immunoexpression of the proliferation-associated antigen Ki-67. Retrospective analysis of adenomas and adenocarcinomas of the large intestine. Immunohistochemistry for M30 and Ki-67, and in situ end-labeling. Formalin-fixed, paraffin-embedded tissue was used. The number of cells positive for M30, Ki-67, and in situ end-labeling, expressed as a proportion of the total number of cells counted. A strong positive correlation was found between in situ end-labeling and expression of M30, although the counts were widely scattered around the regression line. Counts of Ki-67 were strongly correlated with both M30 expression and in situ end-labeling. Immunoexpression of M30 was generally easier to interpret than in situ end-labeling, and the procedures for M30 immunohistochemistry were technically less exacting. These findings support the application of M30 immunoreactivity in the study of apoptosis.

A new regulatory pathway of mRNA export by an F-box protein, Mdm30.

PubMed

Durairaj, Geetha; Lahudkar, Shweta; Bhaumik, Sukesh R

2014-02-01

Mdm30, an F-box protein in yeast, has been recently shown to promote mRNA export. However, it remains unknown how Mdm30 facilitates mRNA export. Here, we show that Mdm30 targets the Sub2 component of the TREX (Transcription/Export) complex for ubiquitylation and subsequent proteasomal degradation. Such a targeted degradation of Sub2 enhances the recruitment of the mRNA export adaptor, Yra1, to the active genes to promote mRNA export. Together, these results elucidate that Mdm30 promotes mRNA export by lowering Sub2's stability and consequently enhancing Yra1 recruitment, thus illuminating new regulatory mechanisms of mRNA export by Mdm30.

33 CFR 117.559 - Isle of Wight (Sinepuxent) Bay.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 4, the draw need not open from 9:30 p.m. until 10:30 p.m. to accommodate the annual July 4th... need not open from 9:30 p.m. until 10:30 p.m. on July 5th to accommodate the annual July 4th fireworks...

33 CFR 117.559 - Isle of Wight (Sinepuxent) Bay.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 4, the draw need not open from 9:30 p.m. until 10:30 p.m. to accommodate the annual July 4th... need not open from 9:30 p.m. until 10:30 p.m. on July 5th to accommodate the annual July 4th fireworks...

Vertical Accuracy Assessment of 30-M Resolution Alos, Aster, and Srtm Global Dems Over Northeastern Mindanao, Philippines

NASA Astrophysics Data System (ADS)

Santillan, J. R.; Makinano-Santillan, M.

2016-06-01

The ALOS World 3D - 30 m (AW3D30), ASTER Global DEM Version 2 (GDEM2), and SRTM-30 m are Digital Elevation Models (DEMs) that have been made available to the general public free of charge. An important feature of these DEMs is their unprecedented horizontal resolution of 30-m and almost global coverage. The very recent release of these DEMs, particularly AW3D30 and SRTM- 30 m, calls for opportunities for the conduct of localized assessment of the DEM's quality and accuracy to verify their suitability for a wide range of applications in hydrology, geomorphology, archaelogy, and many others. In this study, we conducted a vertical accuracy assessment of these DEMs by comparing the elevation of 274 control points scattered over various sites in northeastern Mindanao, Philippines. The elevations of these control points (referred to the Mean Sea Level, MSL) were obtained through 3rd order differential levelling using a high precision digital level, and their horizontal positions measured using a global positioning system (GPS) receiver. These control points are representative of five (5) land-cover classes namely brushland (45 points), built-up (32), cultivated areas (97), dense vegetation (74), and grassland (26). Results showed that AW3D30 has the lowest Root Mean Square Error (RMSE) of 5.68 m, followed by SRTM-30 m (RMSE = 8.28 m), and ASTER GDEM2 (RMSE = 11.98 m). While all the three DEMs overestimated the true ground elevations, the mean and standard deviations of the differences in elevations were found to be lower in AW3D30 compared to SRTM-30 m and ASTER GDEM2. The superiority of AW3D30 over the other two DEMS was also found to be consistent even under different landcover types, with AW3D30's RMSEs ranging from 4.29 m (built-up) to 6.75 m (dense vegetation). For SRTM-30 m, the RMSE ranges from 5.91 m (built-up) to 10.42 m (brushland); for ASTER GDEM2, the RMSE ranges from 9.27 m (brushland) to 14.88 m (dense vegetation). The results of the vertical accuracy assessment suggest that the AW3D30 is more accurate than SRTM-30 m and ASTER GDEM2, at least for the areas considered in this study. On the other hand, the tendencies of the three DEMs to overestimate true ground elevation can be considered an important finding that users of the DEMs in the Philippines should be aware of, and must be considered into decisions regarding use of these data products in various applications.

17 CFR 230.110 - Business hours of the Commission.

Code of Federal Regulations, 2013 CFR

2013-04-01

... open each day, except Saturdays, Sundays, and Federal holidays, from 9 a.m. to 5:30 p.m., Eastern... federal holidays, from 8 a.m. to 5:30 p.m., Eastern Standard Time or Eastern Daylight Saving Time... Commission each day, except Saturdays, Sundays and federal holidays, from 5:30 p.m. to 10 p.m., Eastern...

17 CFR 230.110 - Business hours of the Commission.

Code of Federal Regulations, 2014 CFR

2014-04-01

... open each day, except Saturdays, Sundays, and Federal holidays, from 9 a.m. to 5:30 p.m., Eastern... federal holidays, from 8 a.m. to 5:30 p.m., Eastern Standard Time or Eastern Daylight Saving Time... Commission each day, except Saturdays, Sundays and federal holidays, from 5:30 p.m. to 10 p.m., Eastern...

17 CFR 230.110 - Business hours of the Commission.

Code of Federal Regulations, 2012 CFR

2012-04-01

... open each day, except Saturdays, Sundays, and Federal holidays, from 9 a.m. to 5:30 p.m., Eastern... federal holidays, from 8 a.m. to 5:30 p.m., Eastern Standard Time or Eastern Daylight Saving Time... Commission each day, except Saturdays, Sundays and federal holidays, from 5:30 p.m. to 10 p.m., Eastern...

17 CFR 230.110 - Business hours of the Commission.

Code of Federal Regulations, 2011 CFR

2011-04-01

... open each day, except Saturdays, Sundays, and Federal holidays, from 9 a.m. to 5:30 p.m., Eastern... federal holidays, from 8 a.m. to 5:30 p.m., Eastern Standard Time or Eastern Daylight Saving Time... Commission each day, except Saturdays, Sundays and federal holidays, from 5:30 p.m. to 10 p.m., Eastern...

77 FR 14783 - Notification of a Public Meeting and Two Public Teleconferences for the Clean Air Scientific...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-13

... face-to-face public meeting will be held on Tuesday, April 10, 2012, from 8:30 a.m. to 5:30 p.m. (Eastern Time) and on Wednesday, April 11, 2012, from 8:30 a.m. to 12:30 p.m. (Eastern Time). The follow-up.... to 3 p.m. (Eastern Time). The quality review public teleconference of the Chartered CASAC will be...

Trehalose improves semen antioxidant enzymes activity, post-thaw quality, and fertility in Nili Ravi buffaloes (Bubalus bubalis).

PubMed

Iqbal, Sajid; Andrabi, Syed Murtaza Hassan; Riaz, Amjad; Durrani, Aneela Zameer; Ahmad, Nasim

2016-03-15

Our objectives were to study the effect of trehalose in extender on (1) antioxidant enzymes profile during cryopreservation (after dilution, before freezing, and after thawing), (2) in vitro quality (after thawing), and (3) in vivo fertility of Nili Ravi buffalo (Bubalus bubalis) bull spermatozoa. Semen samples (n = 20) from four buffalo bulls were diluted in Tris-citric acid-based extender having different concentrations of trehalose (0.0, 15, 30, 45, and 60 mM) and frozen in French straws. At post dilution, profile of sperm catalase (U/mL) was higher (P < 0.05) in extenders containing 15, 30, and 45 mM of trehalose as compared to control. Although profiles of superoxide dismutase (U/mL) and total glutathione (μM) were higher (P < 0.05) in extenders containing 15 and 30 mM of trehalose as compared to control. At prefreezing, sperm catalase, superoxide dismutase, and total glutathione profiles were higher (P < 0.05) in all the treatment groups as compared to control. At post thawing, the profiles of catalase and total glutathione were higher (P < 0.05) in extender containing 30-mM trehalose as compared to other treatment groups and control. Whereas, profile of superoxide dismutase was higher (P < 0.05) in extenders containing 30, 45, and 60 mM of trehalose as compared to control and 15mM group. Post thaw total sperm motility (%) was higher (P < 0.05) in extender containing 30-mM trehalose as compared to control and 15 and 60-mM groups. Although sperm progressive motility (%), rapid velocity (%), average path velocity (μm/s), straight line velocity (μm/s), curvilinear velocity (μm/s), plasma membrane (structural and functional, %), acrosome (%), and DNA (%) integrity were higher (P < 0.05) in extender containing 30 mM trehalose as compared to other treatment groups and control. The fertility rates (61% vs. 43%) were higher (P < 0.05) in buffaloes inseminated with semen doses cryopreserved in extender containing 30 mM of trehalose than the control. It is concluded that addition of 30-mM trehalose in extender improves the semen antioxidant enzymes activity, post thaw quality, and fertility in Nili Ravi buffaloes. Copyright © 2016 Elsevier Inc. All rights reserved.

Developing a 30-m grassland productivity estimation map for central Nebraska using 250-m MODIS and 30-m Landsat-8 observations

USGS Publications Warehouse

Gu, Yingxin; Wylie, Bruce K.

2015-01-01

Accurately estimating aboveground vegetation biomass productivity is essential for local ecosystem assessment and best land management practice. Satellite-derived growing season time-integrated Normalized Difference Vegetation Index (GSN) has been used as a proxy for vegetation biomass productivity. A 250-m grassland biomass productivity map for the Greater Platte River Basin had been developed based on the relationship between Moderate Resolution Imaging Spectroradiometer (MODIS) GSN and Soil Survey Geographic (SSURGO) annual grassland productivity. However, the 250-m MODIS grassland biomass productivity map does not capture detailed ecological features (or patterns) and may result in only generalized estimation of the regional total productivity. Developing a high or moderate spatial resolution (e.g., 30-m) productivity map to better understand the regional detailed vegetation condition and ecosystem services is preferred. The 30-m Landsat data provide spatial detail for characterizing human-scale processes and have been successfully used for land cover and land change studies. The main goal of this study is to develop a 30-m grassland biomass productivity estimation map for central Nebraska, leveraging 250-m MODIS GSN and 30-m Landsat data. A rule-based piecewise regression GSN model based on MODIS and Landsat (r = 0.91) was developed, and a 30-m MODIS equivalent GSN map was generated. Finally, a 30-m grassland biomass productivity estimation map, which provides spatially detailed ecological features and conditions for central Nebraska, was produced. The resulting 30-m grassland productivity map was generally supported by the SSURGO biomass production map and will be useful for regional ecosystem study and local land management practices.

76 FR 58782 - Mid-Atlantic Fishery Management Council (MAFMC); Public Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-22

.... SUMMARY: The Mid-Atlantic Fishery Management Council (Council), its Research Set-Aside (RSA) Committee... INFORMATION: On Tuesday, October 11, the RSA Committee will meet from 8:30 a.m. until 10:30 a.m. The SMB..., the Council will convene at 8:30 a.m. The RSA Program Review Report will be heard from 8:30 a.m. until...

33 CFR 117.1061 - Tacoma Harbor.

Code of Federal Regulations, 2011 CFR

2011-07-01

... at least two hours notice is given. However the draw need not be opened from 6:30 a.m. to 8:30 a.m. and 3:30 p.m. to 5:30 p.m. Monday through Friday, except Federal holidays for vessels of less than 1... emergencies, openings shall be made as soon as possible upon notification to the Washington State Department...

75 FR 4594 - Board of Governors; Sunshine Act Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-28

... POSTAL SERVICE Board of Governors; Sunshine Act Meeting DATE AND TIME: Tuesday, February 9, 2010, at 8:30 a.m.; 1:30 p.m. and 3:15 p.m. PLACE: Washington, DC, at U.S. Postal Service Headquarters, 475 L'Enfant Plaza, SW., in the Benjamin Franklin Room. STATUS: February 9 at 8:30 a.m.--Closed; 1:30 p...

30 AND 4HNE ARE SEQUESTERED IN DIFFERENT AGGRESOMES IN THE SAME HEPATOCYTES

PubMed Central

Amidi, Fataneh; French, Barbara A; Chung, David; Halsted, Charles H.; Medici, Valentina; French, Samuel W.

2007-01-01

M-30 and 4HNE adducts are two markers of active liver disease. M-30 is a serologic marker and 4HNE adducts are histologic markers. M-30 is a marker for apoptosis because it is a fragment of cytokeratin-18 left over from proteolysis by caspase 3. 4HNE is a marker of oxidative stress because it results from lipid peroxidation. Both markers are commonly found in nonalcoholic steatohepatitis and in alcoholic hepatitis. Liver biopsies from patients with steatohepatitis, 11 alcoholic and 11 non-alcoholics were stained for 4HNE and M-30. Almost all of the biopsies in both groups showed 4HNE and M-30 positive aggresomes in hepatocytes. Mallory Denk bodies (MDB) stained variably positive for M-30, whereas 4HNE was present in aggresomes independent of MDBs. However, they were sometimes located in hepatocytes which also contained MDBs as shown by confocal microscopy of double stained biopsies. The results indicate that the formation of M-30 and 4HNE aggresomes occurs through different pathways of liver cell injury in both types of steatohepatitis. PMID:17963745

Estimating V̄s(30) (or NEHRP site classes) from shallow velocity models (depths < 30 m)

USGS Publications Warehouse

Boore, David M.

2004-01-01

The average velocity to 30 m [V??s(30)] is a widely used parameter for classifying sites to predict their potential to amplify seismic shaking. In many cases, however, models of shallow shear-wave velocities, from which V??s(30) can be computed, do not extend to 30 m. If the data for these cases are to be used, some method of extrapolating the velocities must be devised. Four methods for doing this are described here and are illustrated using data from 135 boreholes in California for which the velocity model extends to at least 30 m. Methods using correlations between shallow velocity and V??s(30) result in significantly less bias for shallow models than the simplest method of assuming that the lowermost velocity extends to 30 m. In addition, for all methods the percent of sites misclassified is generally less than 10% and falls to negligible values for velocity models extending to at least 25 m. Although the methods using correlations do a better job on average of estimating V??s(30), the simplest method will generally result in a lower value of V??s(30) and thus yield a more conservative estimate of ground motion [which generally increases as V??s(30) decreases].

77 FR 54554 - Notice of Public Meetings of Committees of the Administrative Conference of the United States

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-05

... Administration and Management: Tuesday, September 25, 2012 from 9:30 a.m. to 12:30 p.m. and Wednesday, October 24, 2012, from 2 p.m. to 5 p.m. Committee on Collaborative Governance: Thursday, September 20, 2012 from 3 p.m. to 5 p.m. and Monday, October 15, 2012, from 2 p.m. to 4:30 p.m. Committee on Judicial Review...

33 CFR 117.843 - Trent River.

Code of Federal Regulations, 2010 CFR

2010-07-01

.... 70 bridge, mile 0.0, at New Bern: (1) Need not open from 6:30 a.m. to 8:30 a.m. and from 4:00 p.m. to 6:00 p.m., Monday through Friday, for pleasure vessels. However, the draw shall open at 7:30 a.m. and 5:00 p.m. for any vessel waiting to pass. (2) Need not open from 2:00 p.m. to 7:00 p.m. from May...

33 CFR 117.843 - Trent River.

Code of Federal Regulations, 2012 CFR

2012-07-01

.... 70 bridge, mile 0.0, at New Bern: (1) Need not open from 6:30 a.m. to 8:30 a.m. and from 4:00 p.m. to 6:00 p.m., Monday through Friday, for pleasure vessels. However, the draw shall open at 7:30 a.m. and 5:00 p.m. for any vessel waiting to pass. (2) Need not open from 2:00 p.m. to 7:00 p.m. from May...

33 CFR 117.843 - Trent River.

Code of Federal Regulations, 2013 CFR

2013-07-01

.... 70 bridge, mile 0.0, at New Bern: (1) Need not open from 6:30 a.m. to 8:30 a.m. and from 4:00 p.m. to 6:00 p.m., Monday through Friday, for pleasure vessels. However, the draw shall open at 7:30 a.m. and 5:00 p.m. for any vessel waiting to pass. (2) Need not open from 2:00 p.m. to 7:00 p.m. from May...

33 CFR 117.824 - Neuse River.

Code of Federal Regulations, 2010 CFR

2010-07-01

.... 17 bridge, mile 33.7, at New Bern: (1) Need not open from 6:30 a.m. to 8:30 a.m. and from 4:00 p.m. to 6:00 p.m., Monday through Friday, for pleasure vessels. However, the draw shall open at 7:30 a.m. and 5:00 p.m., for any vessel waiting to pass. (2) Need not open from 2:00 p.m. to 7:00 p.m. from May...

33 CFR 117.843 - Trent River.

Code of Federal Regulations, 2014 CFR

2014-07-01

.... 70 bridge, mile 0.0, at New Bern: (1) Need not open from 6:30 a.m. to 8:30 a.m. and from 4:00 p.m. to 6:00 p.m., Monday through Friday, for pleasure vessels. However, the draw shall open at 7:30 a.m. and 5:00 p.m. for any vessel waiting to pass. (2) Need not open from 2:00 p.m. to 7:00 p.m. from May...

33 CFR 117.843 - Trent River.

Code of Federal Regulations, 2011 CFR

2011-07-01

.... 70 bridge, mile 0.0, at New Bern: (1) Need not open from 6:30 a.m. to 8:30 a.m. and from 4:00 p.m. to 6:00 p.m., Monday through Friday, for pleasure vessels. However, the draw shall open at 7:30 a.m. and 5:00 p.m. for any vessel waiting to pass. (2) Need not open from 2:00 p.m. to 7:00 p.m. from May...

33 CFR 117.824 - Neuse River.

Code of Federal Regulations, 2011 CFR

2011-07-01

.... 17 bridge, mile 33.7, at New Bern: (1) Need not open from 6:30 a.m. to 8:30 a.m. and from 4:00 p.m. to 6:00 p.m., Monday through Friday, for pleasure vessels. However, the draw shall open at 7:30 a.m. and 5:00 p.m., for any vessel waiting to pass. (2) Need not open from 2:00 p.m. to 7:00 p.m. from May...

77 FR 75150 - Department of Defense Task Force on the Care, Management, and Transition of Recovering Wounded...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-19

... Research and Materiel Command TBI Research Efforts Briefing 10:30 a.m.-11:30 a.m. Veteran Affairs VR&E... Health Affairs (HA) 2:30 p.m.-2:45 p.m. Break 2:45 p.m.-4:45 p.m. Defense Centers of Health on Psychological Health and Traumatic Brain Injury (DCoE PH and TBI) Updates 4:45 p.m.-5:00 p.m. Wrap Up Public's...

Regional correlations of VS30 averaged over depths less than and greater than 30 meters

USGS Publications Warehouse

Boore, David M.; Thompson, Eric M.; Cadet, Héloïse

2011-01-01

Using velocity profiles from sites in Japan, California, Turkey, and Europe, we find that the time-averaged shear-wave velocity to 30 m (VS30), used as a proxy for site amplification in recent ground-motion prediction equations (GMPEs) and building codes, is strongly correlated with average velocities to depths less than 30 m (VSz, with z being the averaging depth). The correlations for sites in Japan (corresponding to the KiK-net network) show that VSz is systematically larger for a given VSz than for profiles from the other regions. The difference largely results from the placement of the KiK-net station locations on rock and rocklike sites, whereas stations in the other regions are generally placed in urban areas underlain by sediments. Using the KiK-net velocity profiles, we provide equations relating VS30 to VSz for z ranging from 5 to 29 m in 1-m increments. These equations (and those for California velocity profiles given in Boore, 2004b) can be used to estimate VS30 from VSz for sites in which velocity profiles do not extend to 30 m. The scatter of the residuals decreases with depth, but, even for an averaging depth of 5 m, a variation in logVS30 of ±1 standard deviation maps into less than a 20% uncertainty in ground motions given by recent GMPEs at short periods. The sensitivity of the ground motions to VS30 uncertainty is considerably larger at long periods (but is less than a factor of 1.2 for averaging depths greater than about 20 m). We also find that VS30 is correlated with VSz for z as great as 400 m for sites of the KiK-net network, providing some justification for using VS30 as a site-response variable for predicting ground motions at periods for which the wavelengths far exceed 30 m.

36 CFR 1253.6 - Records Centers.

Code of Federal Regulations, 2010 CFR

2010-07-01

... hours are 8 a.m. to 4:30 p.m., Monday through Friday. The telephone number is 781-663-0139. (b) NARA...:30 p.m. The telephone number is 413-236-3600. (c) NARA—Mid Atlantic Region (Northeast Philadelphia) is located at 14700 Townsend Rd., Philadelphia, PA 19154-1096. The hours are 8 a.m. to 4:30 p.m...

Large Deployable Reflector (LDR)

NASA Technical Reports Server (NTRS)

Alff, W. H.

1980-01-01

The feasibility and costs were determined for a 1 m to 30 m diameter ambient temperature, infrared to submillimeter orbiting astronomical telescope which is to be shuttle-deployed, free-flying, and have a 10 year orbital life. Baseline concepts, constraints on delivery and deployment, and the sunshield required are examined. Reflector concepts, the optical configuration, alignment and pointing, and materials are also discussed. Technology studies show that a 10 m to 30 m diameter system which is background and diffraction limited at 30 micron m is feasible within the stated time frame. A 10 m system is feasible with current mirror technology, while a 30 m system requires technology still in development.

Adaptive statistical iterative reconstruction and Veo: assessment of image quality and diagnostic performance in CT colonography at various radiation doses.

PubMed

Yoon, Min A; Kim, Se Hyung; Lee, Jeong Min; Woo, Hyoun Sik; Lee, Eun Sun; Ahn, Se Jin; Han, Joon Koo

2012-01-01

To evaluate the diagnostic performance of computed tomography (CT) colonography (CTC) reconstructed with different levels of adaptive statistical iterative reconstruction (ASiR, GE Healthcare) and Veo (model-based iterative reconstruction, GE Healthcare) at various tube currents in detection of polyps in porcine colon phantoms. Five porcine colon phantoms with 46 simulated polyps were scanned at different radiation doses (10, 30, and 50 mA s) and were reconstructed using filtered back projection (FBP), ASiR (20%, 40%, and 60%) and Veo. Eleven data sets for each phantom (10-mA s FBP, 10-mA s 20% ASiR, 10-mA s 40% ASiR, 10-mA s 60% ASiR, 10-mA s Veo, 30-mA s FBP, 30-mA s 20% ASiR, 30-mA s 40% ASiR, 30-mA s 60% ASiR, 30-mA s Veo, and 50-mA s FBP) yielded a total of 55 data sets. Polyp detection sensitivity and confidence level of 2 independent observers were evaluated with the McNemar test, the Fisher exact test, and receiver operating characteristic curve analysis. Comparative analyses of overall image quality score, measured image noise, and interpretation time were also performed. Per-polyp detection sensitivities and specificities were highest in 10-mA s Veo, 30-mA s FBP, 30-mA s 60% ASiR, and 50-mA s FBP (sensitivity, 100%; specificity, 100%). The area-under-the-curve values for the overall performance of each data set was also highest (1.000) at 50-mA s FBP, 30-mA s FBP, 30-mA s 60% ASiR, and 10-mA s Veo. Images reconstructed with ASiR showed statistically significant improvement in per-polyp detection sensitivity as the percent level of per-polyp sensitivity increased (10-mA s FBP vs 10-mA s 20% ASiR, P = 0.011; 10-mA s FBP vs 10-mA s 40% ASiR, P = 0.000; 10-mA s FBP vs 10-mA s 60% ASiR, P = 0.000; 10-mA s 20% ASiR vs 40% ASiR, P = 0.034). Overall image quality score was highest at 30-mA s Veo and 50-mA s FBP. The quantitative measurement of the image noise was lowest at 30-mA s Veo and second lowest at 10-mA s Veo. There was a trend of decrease in time required for image interpretation as the percent level of ASiR increased, and ASiR or Veo was used instead of FBP. However, differences from comparative analyses of overall image quality score, measured image noise, and interpretation time did not reach statistical significance. ASiR and Veo showed improved diagnostic performance with excellent sensitivity and specificity with less image noise and good image quality compared with FBP reconstruction of same radiation dose. Our study confirmed feasibility of low-dose CTC with iterative reconstruction as a promising screening tool with excellent diagnostic performance similar to that of the standard-dose CTC with FBP.

MASTER: bright PSN

NASA Astrophysics Data System (ADS)

Pogrosheva, T.; Lipunov, V.; Kornilov, V.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Gress, O.; Gabovich, A.; Gorbunov, I.; Senik, V.; Vladimirov, V.; Vlasenko, D.; Kuvshinov, D.

2018-05-01

MASTER-Tavrida auto-detection system ( Lipunov et al., "MASTER Global Robotic Net",Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 11h 02m 30.30s +55d 35m 55.5s on 2018-05-25.79944 UT. The OT unfiltered magnitude is 17.3m (limit 19.0m).

The Construal of Midwives by Pregnant Women with a Body Mass Index Greater Than or Equal to 30 kg/m2 (BMI ≥ 30 kg/m2 ): A Repertory Grid Study.

PubMed

Hodgkinson, Emma L; Smith, Debbie M; Hare, Dougal Julian; Wittkowski, Anja

2017-03-01

To explore the construal of midwives by pregnant women with a body mass index greater than 30 kg/m 2 (BMI ≥ 30 kg/m 2 ). Ten pregnant women with a BMI ≥ 30 kg/m 2 were recruited from antenatal clinics at a maternity hospital in the North West of England. Each participant completed a repertory grid. The participants chose people to match roles including themselves, pregnant women, midwives of different BMIs and hypothetical elements. They also generated psychological constructs to describe them. Pregnant women with a BMI ≥ 30 kg/m 2 construed themselves as vulnerable and self-conscious. Some women endorsed obesity-related stereotypes for themselves and felt responsible for their weight. The midwife with a BMI 18 < 30 kg/m 2 was considered to be most similar to the ideal midwife, while the midwife with a BMI ≤ 18 kg/m 2 was construed as having an undesirable interpersonal style. The midwife with a BMI ≥ 40 kg/m 2 was often construed as sharing similar experiences to the pregnant women with a BMI ≥ 30 kg/m 2 , such as struggling with the psychological consequences of a raised BMI. Some women construed the midwife with a BMI 30 < 40 kg/m 2 in a positive way, whereas others viewed it as sharing similar feelings about weight as the midwife with a BMI ≥ 40 kg/m 2 . The pregnant women with a BMI ≥ 30 kg/m 2 in this study described perceptions of themselves and the midwives responsible for their care, which may affect their engagement and satisfaction with services. Pregnant women with a BMI ≥ 30 kg/m 2 should be involved in service development activities to ensure the structure of services and the language used by midwives are acceptable and do not confirm weight-related stereotypes. Copyright © 2016 John Wiley & Sons, Ltd. Pregnant women with a BMI ≥ 30 kg/m 2 construe themselves as vulnerable and self-conscious and perceive themselves responsible for their weight. Pregnant women with a BMI ≥ 30 kg/m 2 construe midwives with a low BMI as having an undesirable, cold, interpersonal style. Midwives with a raised BMI are construed as similar to the women, because they share the uncomfortable psychological consequences of a raised BMI. The nature of pregnant women's construal may affect their engagement and satisfaction with maternity services and midwifery care. Copyright © 2016 John Wiley & Sons, Ltd.

[Interspecific interaction between Moina mongolica and Brachionus plicatilis].

PubMed

Chen, Taoying; Wang, Yan; Huang, Changzhi; Hu, Shiheng; Zhang, Jun

2004-07-01

In a laboratory experiment, Moina mongolica and Brachionus plicatilis were polycultured at four relatively inoculating densities, i.e., 0.06/0.30, 0.10/0.30, 0.30/0.30 and 0.60/0.30 (ind x ml(-1)/ind x ml(-1)), while M. mongolica monocultured at 0.06, 0.10, 0.30 and 0.60 ind x ml(-1) and B. plicatilis at 0.30 ind x ml(-1) were used as the control. Interspecific interference did exist between M. mongolica and B. plicatilis when these two species coexisted in a microcosm. In the polycultured microcosms, depressed population density of M. mongolica resulted from the coexistence of B. plicatilis, and M. mongolica maintained at extremely low density. In opposite, M. mongolica had negligibly negative influence on the population of B. plicatilis. Further experiment showed that the mortality of M. mongolica deprived of food for 120 h was 100%, while 90% of B. plicatilis survived after 144 h of food deprivation. The relatively strong capacity of B. plicatilis in tolerating starvation may be one of the important reasons for it wins the competition against M. mongolica.

78 FR 28743 - Safety Zones; Fireworks Displays in the Sector Columbia River Captain of the Port Zone Columbia...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-16

..., OR: May 3, 2013 from 9:30 p.m. until 10 p.m. (2) Portland Rose Festival Fireworks Display, Portland..., Astoria, OR: July 4, 2013, from 10 p.m. until 10:30 p.m. (6) Waterfront Blues Festival Fireworks, Portland...: July 6, 2013, from 10 p.m. until 10:30 p.m. (12) Splash Aberdeen Waterfront Festival, Aberdeen, WA...

The Dorothy I. Height Center for Health Equity and Evaluation Research (CHEER)

DTIC Science & Technology

2013-12-01

Building, Lecture Hall A-436, 4th Floor – One Main Street, Houston, TX 77002 2011 FALL SCHEDULE - REVISED 5:30 p.m. – 8:30 p.m., TUESDAY - WEEKLY...August 23, 2011 – December 6, 2011 August 23, 2011 - Tuesday , 5:30-6:30 p.m., Academic Bldg., Lecture Hall A-436 “Overview of Class Requirements...Center, Houston, TX. 6:30-6:45 - 15 Minute Break August 23, 2011 - Tuesday , 6:45-7:45 p.m., Academic Bldg., Lecture Hall A-436 “Health

Ammunition Cost Research Study. Volume I.

DTIC Science & Technology

1983-01-01

0.048 4,000,000 1.72 1.74 M717 PD 0.25 571,490 8.22 6.13 M567 PD 1.30 300,000 10.43 17.22 M503A2 PD 0.34 814,701 10.64 7.28 M739 PD 1.43 915,837 10.65...M503A2 PD 0.34 252,336 10.73 7.58 M567 PD 1.30 1,071,100 11.23 16.29 M739 PD 1.43 915,837 11.30 17.47 M720 PD 2.10 90,000 11.41 23.46 m567 PD 1.30...14.86 12.91 M524A6 PD 1.27 3,982,150 15.11 13.04 M524A6 PD 1.27 3,181,102 15.44 13.81 M739 PD 1.43 3,168,072 15.47 14.86 M524A6 PD 1.27 3,045,000 17.37

Estimation of Vs30 Soil Profile Structure of Singapore from Microtremor Records

NASA Astrophysics Data System (ADS)

Walling, M. Y.; Megawati, K.; Zhu, C.

2012-04-01

Singapore lies at the southern tip of the Malay Peninsula, covering a land area of 600 km2 and with a population exceeding 5 million. Array microtremor recording were carried out in Singapore for 40 sites that encompasses the sites of all the major geological formations. The Spatial Autocorrelation (SPAC) method is employed to determine the phase velocity dispersion curves and subsequently inverted to determine the shallow shear-wave velocity (V s) and soil stratigraphy. The depth of penetration is generally about 30 m - 40 m for most of the sites. For the present study, the V s estimation is restricted to the upper 30 m of the soil (V s30), confirming with the IBC (2006). The Reclaimed Land and the young Quaternary soft soil deposit of Kallang Formation show low V s30 values ranging from 207 m/s - 247 m/s, belonging to site E and at the boundary of site E and D. The Old Alluvium formation shows higher V s30 values ranging from 362 m/s - 563 m/s and can be classified under site C. The estimated V s30 for the sedimentary sequence of Jurong Formation reveal site C classification, with the V s30 range from 317 m/s - 712 m/s. On the other hand, the Bukit Timah Granite body shows low V s30 ranging from 225 m/s - 387 m/s, with most of the sites concentrated under site D classification and few sites at the boundary of sites D and C, for the upper 30 m. This low V s30 value of the granitic body can be explained in the light of intense weathering that the granite body has undergone for the upper layer, which is also supported from borehole records. The SPAC results are compared with nearby borehole data and they show a good correlation for sites that have soft soil formation and for the weathered granite body. The correlation confirms the reliability of SPAC method that can be applied for highly populated urbanized places like Singapore. The present research finding will be useful for further studies of site response analysis, site characterization and ground motion simulation.

HIV-1 Drug Resistance and Second-Line Treatment in Children Randomized to Switch at Low Versus Higher RNA Thresholds.

PubMed

Harrison, Linda; Melvin, Ann; Fiscus, Susan; Saidi, Yacine; Nastouli, Eleni; Harper, Lynda; Compagnucci, Alexandra; Babiker, Abdel; McKinney, Ross; Gibb, Diana; Tudor-Williams, Gareth

2015-09-01

The PENPACT-1 trial compared virologic thresholds to determine when to switch to second-line antiretroviral therapy (ART). Using PENPACT-1 data, we aimed to describe HIV-1 drug resistance accumulation on first-line ART by virologic threshold. PENPACT-1 had a 2 × 2 factorial design, randomizing HIV-infected children to start protease inhibitor (PI) versus nonnucleoside reverse transcriptase inhibitor (NNRTI)-based ART, and switch at a 1000 copies/mL versus 30,000 copies/mL threshold. Switch criteria were not achieving the threshold by week 24, confirmed rebound above the threshold thereafter, or Center for Disease Control and Prevention stage C event. Resistance tests were performed on samples ≥1000 copies/mL before switch, resuppression, and at 4-years/trial end. Sixty-seven children started PI-based ART and were randomized to switch at 1000 copies/mL (PI-1000), 64 PIs and 30,000 copies/mL (PI-30,000), 67 NNRTIs and 1000 copies/mL (NNRTI-1000), and 65 NNRTI and 30,000 copies/mL (NNRTI-30,000). Ninety-four (36%) children reached the 1000 copies/mL switch criteria during 5-year follow-up. In 30,000 copies/mL threshold arms, median time from 1000 to 30,000 copies/mL switch criteria was 58 (PI) versus 80 (NNRTI) weeks (P = 0.81). In NNRTI-30,000, more nucleoside reverse transcriptase inhibitor (NRTI) resistance mutations accumulated than other groups. NNRTI mutations were selected before switching at 1000 copies/mL (23% NNRTI-1000, 27% NNRTI-30,000). Sixty-two children started abacavir + lamivudine, 166 lamivudine + zidovudine or stavudine, and 35 other NRTIs. The abacavir + lamivudine group acquired fewest NRTI mutations. Of 60 switched to second-line, 79% PI-1000, 63% PI-30,000, 64% NNRTI-1000, and 100% NNRTI-30,000 were <400 copies/mL 24 weeks later. Children on first-line NNRTI-based ART who were randomized to switch at a higher virologic threshold developed the most resistance, yet resuppressed on second-line. An abacavir + lamivudine NRTI combination seemed protective against development of NRTI resistance.

33 CFR 117.465 - Lafourche Bayou.

Code of Federal Regulations, 2013 CFR

2013-07-01

... (Golden Meadow) Bridge, mile 23.9, at Golden Meadow (2) Galliano Pontoon Bridge, mile 27.8, at Galliano (3) SR 308 (South Lafourche (Tarpon)) Bridge, mile 30.6, at Galliano, need not open for the passage of... a.m.; from 2 p.m. to 4 p.m. and from 4:30 p.m. to 5:30 p.m. (4) Cote Blanche Pontoon Bridge, mile 33...

33 CFR 117.465 - Lafourche Bayou.

Code of Federal Regulations, 2014 CFR

2014-07-01

... (Golden Meadow) Bridge, mile 23.9, at Golden Meadow (2) Galliano Pontoon Bridge, mile 27.8, at Galliano (3) SR 308 (South Lafourche (Tarpon)) Bridge, mile 30.6, at Galliano, need not open for the passage of... a.m.; from 2 p.m. to 4 p.m. and from 4:30 p.m. to 5:30 p.m. (4) Cote Blanche Pontoon Bridge, mile 33...

78 FR 38354 - Center for Scientific Review; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-26

...; Member Conflict: Psychological Health, Development and Aging. Date: July 22, 2013. Time: 2:30 p.m. to 4... Obesity. Date: July 24, 2013. Time: 10:00 a.m. to 12:00 p.m. Agenda: To review and evaluate grant... Emphasis Panel; PAR Panel: Biodemography of Aging. Date: July 24, 2013. Time: 12:30 p.m. to 4:30 p.m...

Regional correlations of V s30 and velocities averaged over depths less than and greater than 30 meters

USGS Publications Warehouse

Boore, D.M.; Thompson, E.M.; Cadet, H.

2011-01-01

Using velocity profiles from sites in Japan, California, Turkey, and Europe, we find that the time-averaged shear-wave velocity to 30 m (V S30), used as a proxy for site amplification in recent ground-motion prediction equations (GMPEs) and building codes, is strongly correlated with average velocities to depths less than 30 m (V Sz, with z being the averaging depth). The correlations for sites in Japan (corresponding to the KiK-net network) show that V S30 is systematically larger for a given V Sz than for profiles from the other regions. The difference largely results from the placement of the KiK-net station locations on rock and rocklike sites, whereas stations in the other regions are generally placed in urban areas underlain by sediments. Using the KiK-net velocity profiles, we provide equations relating V S30 to V Sz for z ranging from 5 to 29 m in 1-m increments. These equations (and those for California velocity profiles given in Boore, 2004b) can be used to estimate V S30 from V Sz for sites in which velocity profiles do not extend to 30 m. The scatter of the residuals decreases with depth, but, even for an averaging depth of 5 m, a variation in log V S30 of 1 standard deviation maps into less than a 20% uncertainty in ground motions given by recent GMPEs at short periods. The sensitivity of the ground motions to V S30 uncertainty is considerably larger at long periods (but is less than a factor of 1.2 for averaging depths greater than about 20 m). We also find that V S30 is correlated with V Sz for z as great as 400 m for sites of the KiK-net network, providing some justification for using V S30 as a site-response variable for predicting ground motions at periods for which the wavelengths far exceed 30 m.

75 FR 69473 - Sunshine Act Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-12

... Considered December 2 9 a.m.-9:30 a.m. Welcome and Introductions 9:30 a.m.-1 p.m. Strategic Planning 1 p.m.-3 p.m. Forum Planning 3 p.m.-5 p.m. Report and Discussion on Health Care Reform Working Group December...

75 FR 13313 - Advisory Committee for Geosciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-19

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Geosciences; Notice of Meeting In accordance with the Federal Advisory Committee Act (Pub. L. 92- 463, as amended), the National Science Foundation...:30 a.m.-5 p.m. April 15, 2010 8:30, a.m.-1:30 p.m. Place: Stafford I, Room 1235, National Science...

33 CFR 117.621 - Fore River.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Weymouth SR3A bridge, mile 3.5 between Quincy Point and North Weymouth, Massachusetts, shall open on signal, except that: (a) From 6:30 a.m. to 9 a.m. and from 4:30 p.m. to 6:30 p.m., Monday through Friday, except... times for self-propelled vessels greater than 10,000 gross tons. (c) From noon to 6 p.m. on Thanksgiving...

33 CFR 117.621 - Fore River.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Weymouth SR3A bridge, mile 3.5 between Quincy Point and North Weymouth, Massachusetts, shall open on signal, except that: (a) From 6:30 a.m. to 9 a.m. and from 4:30 p.m. to 6:30 p.m., Monday through Friday, except... times for self-propelled vessels greater than 10,000 gross tons. (c) From noon to 6 p.m. on Thanksgiving...

33 CFR 117.621 - Fore River.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Weymouth SR3A bridge, mile 3.5 between Quincy Point and North Weymouth, Massachusetts, shall open on signal, except that: (a) From 6:30 a.m. to 9 a.m. and from 4:30 p.m. to 6:30 p.m., Monday through Friday, except... times for self-propelled vessels greater than 10,000 gross tons. (c) From noon to 6 p.m. on Thanksgiving...

33 CFR 117.621 - Fore River.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Weymouth SR3A bridge, mile 3.5 between Quincy Point and North Weymouth, Massachusetts, shall open on signal, except that: (a) From 6:30 a.m. to 9 a.m. and from 4:30 p.m. to 6:30 p.m., Monday through Friday, except... times for self-propelled vessels greater than 10,000 gross tons. (c) From noon to 6 p.m. on Thanksgiving...

33 CFR 117.621 - Fore River.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Weymouth SR3A bridge, mile 3.5 between Quincy Point and North Weymouth, Massachusetts, shall open on signal, except that: (a) From 6:30 a.m. to 9 a.m. and from 4:30 p.m. to 6:30 p.m., Monday through Friday, except... times for self-propelled vessels greater than 10,000 gross tons. (c) From noon to 6 p.m. on Thanksgiving...

77 FR 66713 - Special Local Regulation; Southern California Annual Marine Events for the San Diego Captain of...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-07

... enforce the Special Local Regulations in 33 CFR 100.1101 from 7 a.m. to 11:30 a.m. on November 11, 2012 on....1101 will be enforced on November 11, 2012 from 7 a.m. until 11:30 a.m. FOR FURTHER INFORMATION CONTACT... in 33 CFR 100.1101 on November 11, 2012 from 7 a.m. to 11:30 a.m. in support of the annual San Diego...

Expression of Momordica charantia MAP30 and its antitumor effect on bladder cancer cells.

PubMed

Hlin, Hao; Zhi-Guo, Zhang; Cong-Hui, Han; Yan, Zhao; Qing, Liang; Bo, Jiang; Hou-Guang, He; Jun-Jie, Zhang; Pei-Ying, Zhang

2016-06-01

Momordica charantia (MC) is an edible medicinal plant that is known for its diversified biological functions. Momordica Antiviral Protein 30kD (MAP30) is a type I single chain ribosome-inactivating protein (RIP) isolated from the mature fruit and seeds of MC. Since MAP30 content in MC is limited, the study aim was to generate the recombinant MAP30 protein using prokaryotic expression system and determine its apoptotic/growth inhibitory effects on bladder cancer 5637 cells. MAP30 gene was amplified by PCR from MC genomic DNA and identified by sequencing. The target gene was inserted into pET-28a (+) vector and transformed into E. coli BL21 (DE3) cells. Positive clones were selected by PCR. Recombinant protein was efficiently expressed under induction with 1.0 mM Isopropylthio-β-D-galactoside (IPTG) at 30° C for 4 hours. Cytotoxicity studies were performed using MTT assay by treating 5637 bladder cancer cells with 100 µg/mL, 200 µg/mL, and 400 µg/mL concentrations of MAP30 for 24 hours and 48 hours, respectively. Flow cytometry was used to measure the apoptosis of MAP30-treatedcells in time course experiments. Full-length MAP30 gene was successfully expressed in Escherichia coli (E. coli) BL21 strain and MAP30 recombinant protein inhibited the growth of bladder cancer 5637 cells at 200 µg/mL and 400 µg/mL concentrations by inducing apoptosis of target cells in a dose- and time-dependent manner. It was, therefore, concluded that the MAP30 recombinant protein displayed potent antitumor activity in vitro.

Value of soluble CD30 in liver transplantation.

PubMed

Fábrega, E; Unzueta, M G; Cobo, M; Casafont, F; Amado, J A; Romero, F P

2007-09-01

CD30 is a membrane glycoprotein that belongs to the tumor necrosis factor superfamily. It is expressed on activated T cells. After activation of CD30(+) T cells, a soluble form of CD30 (sCD30) released into the bloodstream, can be measured in the serum. The aim of our study was to investigate the time course of serum levels of sCD30 during hepatic allograft rejection. Serum levels of sCD30 were determined in 30 healthy subjects and 50 hepatic transplant recipients. These patients were divided into two groups: group I, 35 patients without rejection; and group II, 15 patients with acute rejection. Samples were collected on day 1 and 7 after transplantation and on the day of liver biopsy. The concentrations of sCD30 were similar in the rejection (40.4 +/- 16.5 U/mL) and nonrejection groups (43.0 +/- 18.2 U/mL) on postoperative day 1. We observed a significant increase in sCD30 levels in the rejection group on postoperative day 7 (76.3 +/- 61.8 U/mL vs 46.8 +/- 20.5 U/mL; P = .01). The difference increased when a diagnosis of acute rejection had been established: namely 133.0 +/- 113.5 U/mL versus 40.1 +/- 22.0 U/mL; (P = .001). These levels were also significantly higher during the entire postoperative period in all the patients, with or without rejection, than those observed in healthy controls (26.6 +/- 5.3 U/mL; P = .005). The release of circulating sCD30 is a prominent feature coinciding with the first episode of hepatic allograft rejection. So, monitoring of sCD30 levels may be useful for the early diagnosis of an acute rejection episode.

78 FR 24239 - Advisory Committee for Engineering; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-24

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Engineering; Notice of Meeting In accordance with Federal Advisory Committee Act (Pub. L. 92-463, as amended), the National Science Foundation... Engineering (1115). Date/Time: May 16, 2013: 12:30 p.m. to 5:30 p.m., May 17, 2013: 8:30 a.m. to 2:00 p.m...

78 FR 21861 - Tribal Consultation on the Draft Regulations Governing the Tribal Transportation Program

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-12

... regarding the following topics: On July 6, 2012, Moving Ahead for Progress in the 21st Century Act (MAP-21...:30 p.m. May 16, 2013 Phoenix, AZ 9 a.m.-4:30 p.m. May 21, 2013 Minneapolis, MN 9 a.m.-4:30 p.m... President Obama and became effective on October 1, 2012. Section 1119 of MAP-21 struck the existing laws...

78 FR 54371 - Open Meeting of the Federal Advisory Committee on Insurance

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-03

... 20220, from 1:30-4:30 p.m. Eastern Time. The meeting is open to the public, and the site is accessible..., from 1:30-4:30 p.m. Eastern Time. ADDRESSES: The Federal Advisory Committee on Insurance meeting will...:00 p.m. Eastern Time on Friday, September 13, 2013, to inform the Office that they would like to...

A TALE OF THREE MYSTERIOUS SPECTRAL FEATURES IN CARBON-RICH EVOLVED STARS: THE 21 μm, 30 μm, AND “UNIDENTIFIED INFRARED” EMISSION FEATURES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mishra, Ajay; Li, Aigen; Jiang, B. W., E-mail: amishra@mail.missouri.edu, E-mail: lia@missouri.edu, E-mail: bjiang@bnu.edu.cn

2015-03-20

The mysterious “21 μm” emission feature seen almost exclusively in the short-lived protoplanetary nebula (PPN) phase of stellar evolution remains unidentified since its discovery two decades ago. This feature is always accompanied by the equally mysterious, unidentified “30 μm” feature and the so-called “unidentified infrared” (UIR) features at 3.3, 6.2, 7.7, 8.6, and 11.3 μm which are generally attributed to polycyclic aromatic hydrocarbon (PAH) molecules. The 30 μm feature is commonly observed in all stages of stellar evolution from the asymptotic giant branch through PPN to the planetary nebula phase. We explore the interrelations among the mysterious 21, 30 μm,more » and UIR features of the 21 μm sources. We derive the fluxes emitted in the observed UIR, 21, and 30 μm features from published Infrared Space Observatory or Spitzer/IRS spectra. We find that none of these spectral features correlate with each other. This argues against a common carrier (e.g., thiourea) for both the 21 μm feature and the 30 μm feature. This also does not support large PAH clusters as a possible carrier for the 21 μm feature.« less

78 FR 21346 - Emerging Technology and Research Advisory Committee; Notice of Partially Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-10

...:30 a.m. 1. Opening Remarks. 2. Panel Discussion on Deemed Exports. 3. Emerging Technologies--Results..., April 26 Open Session: 8:30 a.m.-3:30 p.m. 1. Discussion on status of the Export Control Reform...

77 FR 59938 - Center for Scientific Review Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-01

... Panel; Program Project: Drug Addiction. Date: October 30-31, 2012. Time: 8:00 a.m. to 8:30 p.m. Agenda... Biomedical Computational Science and Technology Initiative. Date: October 30, 2012. Time: 3:00 p.m. to 4:00 p...

Essentials of MDS 3.0 section M: skin conditions.

PubMed

Levine, Jeffrey M; Roberson, Sharon; Ayello, Elizabeth A

2010-06-01

To provide information about the impending changes in the Minimum Data Set (MDS) Section M on skin conditions and its implications for practice. This continuing education activity is intended for physicians and nurses with an interest in skin and wound care. After participating in this educational activity, the participant should be better able to: 1. Compare section M of MDS version 2.0 to MDS version 3.0. 2. Apply the complexities of the MDS 3.0 section M for coding. 3. Demonstrate accurate and complete documentation of wounds as per MDS 3.0 section M.

Proceedings of Symposium on Physics of Target Implosion and Pulsed Power Techniques Held in Yokohama, Japan on 30 September-1 October 1987

DTIC Science & Technology

1988-02-01

Japan. -- ,, mnm mmmm m m mil II m mlmmmm PREFACE A It is reported that by using the Nd glass laser, experiments of beam-target interaction are carried...Beam 8. 14.00-14.30 T. Ishimoto and T. Kato (Waseda Univ.) Electron Current Effect on Stability of Plasma Channel 9. 14.30-15.00 S. Kawata, M ...Matsumoto and Y. Masubuchi (Tech. Univ. Nagaoka) Numerical Simulation in LIB ICF 10. 15.00-15.30 J. M . Perlado (Univ. Politec. Madrid) Simulation Code for ICF

75 FR 35648 - Safety Zones; Annual Fireworks Events in the Captain of the Port Detroit Zone

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-23

... regulation will also be enforced from 9:30 p.m. to 11 p.m. on July 4, 2010. Sec. 165.941(a)(37) Caseville... weather on July 3, 2010, this regulation will also be enforced from 9:30 p.m. to 11 p.m. on July 4, 2010... regulation will also be enforced from 9:30 p.m. to 11 p.m. on July 4, 2010. Sec. 165.941(a)(36) Grosse Point...

A 1400 MHz sky survey. II - Confusion-limited maps covering ascension between 19 H 30 M and 7 H 30 m, declination between -5 deg and +82 deg

NASA Astrophysics Data System (ADS)

Condon, J. J.; Broderick, J. J.

1986-05-01

The NRAO 91 m transit telescope and rebuilt four-feed receiver were used to make a 1400 MHz continuum survey with 12.7 arcmin x 11.1 arcmin resolution. New maps covering ascension between 19 h 30 m and 7 h 30 m supplement those in the right ascension range between 7 h 30 m and 19 h 30 m (Condon and Broderick, 1985) to complete the roughly 6.8 sr declination band between -5 deg and +82 deg. Both sets of maps are confusion-limited and contain roughly 3000 sources per sr stronger than 0.15 Jy. They are available on FITS tapes and can be displayed and analyzed with standard AIPS programs. A procedure for making radio 'identifications' automatically from the map tapes is given. A 1400 MHz radio sky atlas of contour plots and coordinate-grid overlays covering declination between -5 deg and +82 deg with a roughly 200 arcsec/mm scale was also produced.

77 FR 40588 - Pacific Fishery Management Council; Public Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-10

... Council's (Council) Ad Hoc Amendment 24 Workgroup will hold a work session, which is open to the public. DATES: The Workgroup's work session will occur from 1 p.m. to 4:30 p.m. on Wednesday, August 1 and continue on Thursday, August 2, 2012, from 8:30 a.m. to 4:30 p.m. ADDRESSES: The work sessions will be held...

76 FR 45516 - Gulf of Mexico Fishery Management Council (Council); Public Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-29

... meeting will be held August 15-19, 2011. ADDRESSES: The meeting will be held at the Crowne Plaza Hotel... Collection; Budget/Personnel; and Administrative Policy. 12:30 p.m.-4:30 p.m.--The Council will receive... Panels. 9 a.m.-9:30 a.m.--Closed Session--The Budget/Personnel Committee/ Full Council will meet to...

Electronic Structure of I-M8Ga16Sn30 (M = Ba, Sr, Yb) by First-Principles Calculation

NASA Astrophysics Data System (ADS)

Wang, Jin-song; Liu, Hong-xia; Deng, Shuping; Li, De-cong; Shen, Lan-xian; Cheng, Feng; Deng, Shu-kang

2017-05-01

Sn-based clathrates possess excellent thermoelectric properties ascribed to their higher Seebeck coefficient and lower thermal conductivity. Guest atoms significantly modulate the thermoelectric properties of Sn-based calculates because of their diverse atomic radius and interactions with framework atoms. Thus, we explored the electronic structure of I-M8Ga16Sn30 (M = Ba, Sr, Yb) by first-principles calculation. Results revealed significant differences between Yb8Ga16Sn30 and M8Ga16Sn30 (M = Ba, Sr,). In particular, the Yb-filled compound substitution possesses lowest formation energy and the off-center distance of the Yb atom is the largest compared with the other structures. I-M8Ga16Sn30 (M = Ba, Sr, Yb) is an indirect band gap semiconductor, and the enhanced hybridization effect between the guest and framework atoms' orbits exists because the Yb f orbit results in a decrease in band gap. Ba- and Sr-filled clathrates have similar valence bands but slightly different conduction bands; however, Yb8Ga16Sn30 possess the spiculate density of states near the Fermi level that reveals excellent thermoelectric properties.

High serum soluble CD30 does not predict acute rejection in liver transplant patients.

PubMed

Matinlauri, I; Höckerstedt, K; Isoniemi, H

2006-12-01

Increased pre- and posttransplantation values of soluble CD30 (sCD30) have been shown to be associated with acute kidney transplant rejection. We sought to study whether high sCD30 could predict rejection early after liver transplantation. The study population included 54 consecutive liver transplant patients, whose samples were collected before liver transplantation and at discharge, which was at a mean time of 3 weeks after transplantation. During the first 6 months posttransplantation, 22 patients experienced an acute rejection episode. Serum sCD30 concentrations were measured by an enzyme-linked immunoassay; changes in serum sCD30 levels posttransplantation were also expressed as relative values compared with pretransplantation results. Liver patients before transplantation displayed higher serum sCD30 values compared with healthy controls: mean values +/- SD were 93 +/- 58 IU/mL vs 17 +/- 8 IU/mL, respectively. At 3 weeks after transplantation the mean sCD30 concentration in liver transplant patients decreased to 59 +/- 42 IU/mL (P = .005). The mean pretransplantation serum sCD30 value was slightly lower among rejecting vs nonrejecting patients: 78 +/- 43 IU/mL vs 104 +/- 65 IU/mL (P = NS). Posttransplantation values in both groups decreased significantly: 47 +/- 34 IU/mL in patients with rejection (P = .014) vs 69 +/- 45 IU/mL in patients without rejection (P = .012). The relative value at 3 weeks posttransplantation decreased slightly more among patients with vs without rejection (70% vs 88%; NS). No correlation was found between serum sCD30 and anti-HLA class I antibodies or crossmatch positivity. In conclusion, neither pre- nor posttransplantation sCD30 levels were associated with acute rejection in liver transplant patients.

International Symposium on Molecular Spectroscopy (70th)

DTIC Science & Technology

2015-06-22

N DAY June 22 8:30 AM TU ESDAY June 23 8:30 AM W EDN ESDAY June 24 8:30 AM THU RSDAY June 25 8:30 AM FRIDAY June 26 8:30 AM Foellinger Auditorium M A...energy surfaces Room 100 N oyes Laboratory TB M ini-sym posium : Accelerator-Based Spectroscopy RB M ini-sym posium : Accelerator-Based...Conform ers, isom ers, chirality, stereochem istry RD Astronom y FD Atm ospheric science Room 217 N oyes Laboratory TE Instrum

Elevation validation and geomorphic metric comparison with focus on ASTER GDEM2, SRTM- C, ALOS World 3D, and TanDEM-X

NASA Astrophysics Data System (ADS)

Purinton, Benjamin; Bookhagen, Bodo

2017-04-01

Geomorphologists use digital elevation models (DEMs) to quantify changes in topography - often without rigorous accuracy assessments. In this study we validate and compare elevation accuracy and derived geomorphic metrics from the current generation of satellite-derived DEMs on the southern Central Andean Plateau. The average elevation of 3.7 km, diverse topography and relief, lack of vegetation, and clear skies create ideal conditions for remote sensing in this study area. DEMs at resolutions of 5-30 m are sourced from open-access, research agreement, and commercial outlets, with a focus on the 30 m SRTM-C, 30 m ASTER GDEM2, 12 m TanDEM-X, and 5 m ALOS World 3D data. In addition to these edited products, manually generated DEMs included 10 m single-CoSSC TerraSAR-X / TanDEM-X DEMs and a 30 m stacked ASTER L1A stereopair DEM. We assessed vertical accuracy by comparing standard deviations (SD) of the DEM elevation versus 307,509 differential GPS (dGPS) measurements with < 0.5 m vertical accuracy, acquired across 4,000 m of elevation. Vertical SD was 3.33 m, 9.48 m, 6.93 m, 1.97 m, 2.02-3.83 m, and 1.64 m for the 30 m SRTM-C, 30 m ASTER GDEM2, 30 m stacked ASTER, 12 m TanDEM-X, 10 m single-CoSSC TerraSAR-X / TanDEM-X DEMs, and 5 m ALOS World 3D, respectively. Analysis of vertical uncertainty with respect to terrain elevation, slope, and aspect revealed the high performance across these attributes of the 30 m SRTM-C, 12 m TanDEM-X, and 5 m ALOS World 3D DEMs. The 10 m single-CoSSC TerraSAR-X / TanDEM-X DEMs and the 30 m ASTER GDEM2 displayed slight aspect biases, which were removed in their stacked counterparts (TanDEM-X and the stacked ASTER DEMs). We selected the high quality 30 m SRTM-C, 12 m TanDEM-X, and 5 m ALOS World 3D for geomorphic metric comparison in a 66 sqkm catchment with a clear river knickpoint. For trunk channel profiles analyzed with chi plots, consistent m/n values of 0.49-0.57 were found regardless of DEM resolution or SD. Hillslopes were analyzed upstream and downstream of the knickpoint by calculating slope and curvature distributions and plotting slope, curvature, and drainage area to assess the hillslope-to-valley transition. While slope and hillslope length measurements vary little between datasets, curvature displays higher magnitude measurements with fining resolution. To assess DEM noise and periodicity in the landscape we employed a Fourier analysis to identify DEM frequencies and their spectral power. The optical 5 m ALOS World 3D DEM shows high-frequency noise in 2-8 pixel steps, with no corresponding landscape features in this highly diffusive, vegetation-free environment. Finally, we explore the geomorphometric potential of the higher-quality 12 m TanDEM-X DEM through a hillslope length and surface roughness assessment across steep environmental, climatic and topographic gradients in the Quebrada del Toro catchment, west of Salta, Argentina.

Surface seismic measurements of near-surface P-and S-wave seismic velocities at earthquake recording stations, Seattle, Washington

USGS Publications Warehouse

Williams, R.A.; Stephenson, W.J.; Frankel, A.D.; Odum, J.K.

1999-01-01

We measured P-and S-wave seismic velocities to about 40-m depth using seismic-refraction/reflection data on the ground surface at 13 sites in the Seattle, Washington, urban area, where portable digital seismographs recently recorded earthquakes. Sites with the lowest measured Vs correlate with highest ground motion amplification. These sites, such as at Harbor Island and in the Duwamish River industrial area (DRIA) south of the Kingdome, have an average Vs in the upper 30 m (V??s30) of 150 to 170 m/s. These values of V??s30 place these sites in soil profile type E (V??s30 < 180 m/s). A "rock" site, located at Seward Park on Tertiary sedimentary deposits, has a V??S30 of 433 m/s, which is soil type C (V??s30: 360 to 760 m/s). The Seward Park site V??s30 is about equal to, or up to 200 m/s slower than sites that were located on till or glacial outwash. High-amplitude P-and S-wave seismic reflections at several locations appear to correspond to strong resonances observed in earthquake spectra. An S-wave reflector at the Kingdome at about 17 to 22 m depth probably causes strong 2-Hz resonance that is observed in the earthquake data near the Kingdome.

76 FR 13601 - Shasta County Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-14

...) will meet at the USDA Service Center in Redding, California, on March 30, 2011, from 8:30 a.m. to 12... monitoring efforts, and a timeline for the upcoming year. DATES: Wednesday, March 30 at 8:30 a.m. ADDRESSES...

48 CFR 515.204-1 - Uniform contract format.

Code of Federal Regulations, 2011 CFR

2011-10-01

... CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Solicitation and Receipt of Proposals and.... to 4:30 p.m. Requests for preaward debriefings postmarked or otherwise submitted after 4:30 p.m. will... after 4:30 p.m. will be considered received and filed the following business day.” ...

75 FR 11870 - Meeting of the Ocean Research and Resources Advisory Panel

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-12

... and Resources Advisory Panel (ORRAP) will hold its first regularly scheduled meeting of the year. The meeting will be open to the public. DATES: The meeting will be held on Monday, March 15, 2010, from 8:30 a.m. to 5:30 p.m. and on Tuesday, March 16, 2010, from 8:30 a.m. to 2 p.m. ADDRESSES: The meeting will...

76 FR 34862 - Safety Zones: Fireworks Displays in the Captain of the Port Columbia River Zone

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-15

....1315 will be enforced as follows: (1) Portland Rose Festival Fireworks Display, Portland, OR: From 8:30..., Astoria, OR: From 8:30 p.m. until 11:30 p.m. on July 4, 2011. (5) Oregon Food Bank Blues Festival..., 2011 from 9 p.m. to 11 p.m. (11) Splash Aberdeen Waterfront Festival, Aberdeen, WA: On July 4, 2011...

Polyoxotungstates now also with pentagonal units: supramolecular chemistry and tuning of magnetic exchange in {(M)M5}12V30 Keplerates (M = Mo, W).

PubMed

Todea, Ana Maria; Merca, Alice; Bögge, Hartmut; Glaser, Thorsten; Engelhardt, Larry; Prozorov, Ruslan; Luban, Marshall; Müller, Achim

2009-06-21

The deliberate synthesis of the Keplerate [K(20) subset{(W)W(5)O(21)(SO(4))}(12)(VO)(30)(SO(4))(H(2)O)(63)](18-) with 20 pores all closed by K(+) in a supramolecular fashion proves that it is possible to follow new routes in polyoxotungstate chemistry based on pentagonal {(W)W(5)}-type units and to tune magnetic exchange couplings in {(M)M(5)}(12)M'(30) type Keplerates.

76 FR 7182 - National Assessment Governing Board; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-09

... Session--12 p.m.-4:15 p.m. Executive Committee: Open Session--4:30 p.m. to 5:15 p.m.; Closed Session--5:15 p.m. to 6 p.m. March 4: Full Board: Open Session--8:15 a.m. to 10 a.m.; Closed Session-- 12:45 p.m...: Closed Session--10:20 a.m. to 12:0 p.m.; Open session 12:25 p.m.-12:30 p.m. Committee on Standards...

Up-regulation of hypoxia-inducible factor (HIF)-1α and HIF-target genes in cortical neurons by the novel multifunctional iron chelator anti-Alzheimer drug, M30.

PubMed

Avramovich-Tirosh, Y; Bar-Am, O; Amit, T; Youdim, M B H; Weinreb, O

2010-06-01

Based on a multimodal drug design paradigm, we have synthesized a multifunctional non-toxic, brain permeable iron chelator, M30, possessing the neuroprotective propargylamine moiety of the anti-Parkinsonian drug, rasagiline (Azilect) and antioxidant-iron chelator moiety of an 8-hydroxyquinoline derivative of our iron chelator, VK28. M30 was recently found to confer potential neuroprotective effects in vitro and in various preclinical neurodegenerative models and regulate the levels and processing of the Alzheimer's amyloid precursor protein and its toxic amyloidogenic derivative, Abeta. Here, we show that M30 activates the hypoxia-inducible factor (HIF)-1alpha signaling pathway, thus promoting HIF-1alpha mRNA and protein expression levels, as well as increasing transcription of HIF-1alpha-dependent genes, including vascular endothelial growth factor, erythropoietin, enolase-1, p21 and tyrosine hydroxylase in rat primary cortical cells. In addition, M30 also increased the expression levels of the transcripts of brain derived neurotrophic factor (BDNF) and growth-associated protein-43 (GAP-43). Regarding aspects of relevance to Alzheimer's disease (AD), western blotting analysis of glycogen synthase kinase- 3beta (GSK-3beta) signaling pathway revealed that M30 enhanced the levels of phospho-AKT (Ser473) and phospho- GSK-3beta (Ser9) and attenuated Tau phosphorylation. M30 was also shown to protect cultured cortical neurons against Abeta(25-35) toxicity. All these multimodal pharmacological activities of M30 might be beneficial for its potent efficacy in the prevention and treatment of neurodegenerative conditions, such as Parkinson's disease and AD in which oxidative stress and iron-mediated toxicity are involved.

Assessing the utility of meteorological drought indices in monitoring summer drought based on soil moisture in Chongqing, China

NASA Astrophysics Data System (ADS)

Chen, Hui; Wu, Wei; Liu, Hong-Bin

2018-04-01

Numerous drought indices have been developed to analyze and monitor drought condition, but they are region specific and limited by various climatic conditions. In southwest China, summer drought mainly occurs from June to September, causing destructive and profound impact on agriculture, society, and ecosystems. The current study assesses the availability of meteorological drought indices in monitoring summer drought in this area at 5-day scale. The drought indices include the relative moisture index ( M), the standardized precipitation index (SPI), the standardized precipitation evapotranspiration index (SPEI), the composite index of meteorological drought (CIspi), and the improved composite index of meteorological drought (CIwap). Long-term daily precipitation and temperature from 1970 to 2014 are used to calculate 30-day M ( M 30), SPI (SPI30), SPEI (SPEI30), 90-day SPEI (SPEI90), CIspi, and CIwap. The 5-day soil moisture observations from 2010 to 2013 are applied to assess the performance of these drought indices. Correlation analysis, overall accuracy, and kappa coefficient are utilized to investigate the relationships between soil moisture and drought indices. Correlation analysis indicates that soil moisture is well correlated with CIwap, SPEI30, M 30, SPI30, and CIspi except SPEI90. Moreover, drought classifications identified by M 30 are in agreement with that of the observed soil moisture. The results show that M 30 based on precipitation and potential evapotranspiration is an appropriate indicator for monitoring drought condition at a finer scale in the study area. According to M 30, summer drought during 1970-2014 happened in each year and showed a slightly upward tendency in recent years.

33 CFR 117.822 - Beaufort Channel, NC.

Code of Federal Regulations, 2010 CFR

2010-07-01

... DRAWBRIDGE OPERATION REGULATIONS Specific Requirements North Carolina § 117.822 Beaufort Channel, NC. The... bridge need not open between the hours of 6:30 a.m. to 8 a.m. and 4:30 p.m. to 6 p.m. (b) From 10 p.m. to...

77 FR 9731 - Voluntary Service National Advisory Committee; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-17

...) National Advisory Committee (NAC) will be held March 14-16, 2012, at the Charleston Marriott, 170 Lockwood Boulevard, Charleston, South Carolina. On March 14, the meeting will begin at 8 a.m. and end at 11:30 a.m. On March 15-16, the meeting will begin at 8:30 a.m. and end at 4:30 p.m. The meeting is open to the...

77 FR 71023 - Self-Regulatory Organizations; New York Stock Exchange LLC; Notice of Filing and Immediate...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-28

... Acceptance Cut-Off Time Cannot Be Past 4:30 p.m. November 21, 2012. Pursuant to Section 19(b)(1) of the... that the order acceptance cut-off time cannot be past 4:30 p.m. The text of the proposed rule change is... order acceptance cut-off time cannot be past 4:30 p.m. (or 30 minutes after the scheduled close in the...

76 FR 53714 - Notice of Request for the Approval of a New Information Collection

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-29

... Transportation, 1200 New Jersey Avenue, SE., Docket Operations, M-30, West Building, Ground Floor, Room W12- 140...., Docket Operations, M-30, West Building, Ground Floor, Room W12-140, Washington, DC 20590-0001 between 9 a... New Jersey Avenue, SE., Docket Operations, M-30, West Building, [[Page 53715

77 FR 41204 - Sunshine Act Meetings; National Science Board

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-12

... Activities Introduction of Chapter Authors and Discussion of Science and Engineering Indicators 2014... introduction) 8:20-8:30 a.m. (SCF) 8:30-11:00 a.m. (previously noticed continuation of CPP meeting) 11:00-11:45... Chairman's Closing Remarks Committee on Science & Engineering Indicators (SEI) Open Session: 1:30-4:00 p.m...

76 FR 41693 - Safety Zone; Annual Events Requiring Safety Zones in Milwaukee Harbor, Milwaukee, WI

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-15

..., 2010 from 10 p.m. through 10:45 p.m. (2) German Festival fireworks display on July 29, 2011 from 9:45 p.m. through 10:30 p.m.; on July 30, 2011 from 10:15 p.m. through 11 p.m. (3) Irish Festival fireworks...

Ground and surface temperature variability for remote sensing of soil moisture in a heterogeneous landscape

USGS Publications Warehouse

Giraldo, M.A.; Bosch, D.; Madden, M.; Usery, L.; Finn, M.

2009-01-01

At the Little River Watershed (LRW) heterogeneous landscape near Tifton Georgia US an in situ network of stations operated by the US Department of Agriculture-Agriculture Research Service-Southeast Watershed Research Lab (USDA-ARS-SEWRL) was established in 2003 for the long term study of climatic and soil biophysical processes. To develop an accurate interpolation of the in situ readings that can be used to produce distributed representations of soil moisture (SM) and energy balances at the landscape scale for remote sensing studies, we studied (1) the temporal and spatial variations of ground temperature (GT) and infra red temperature (IRT) within 30 by 30 m plots around selected network stations; (2) the relationship between the readings from the eight 30 by 30 m plots and the point reading of the network stations for the variables SM, GT and IRT; and (3) the spatial and temporal variation of GT and IRT within agriculture landuses: grass, orchard, peanuts, cotton and bare soil in the surrounding landscape. The results showed high correlations between the station readings and the adjacent 30 by 30 m plot average value for SM; high seasonal independent variation in the GT and IRT behavior among the eight 30 by 30 m plots; and site specific, in-field homogeneity in each 30 by 30 m plot. We found statistical differences in the GT and IRT between the different landuses as well as high correlations between GT and IRT regardless of the landuse. Greater standard deviations for IRT than for GT (in the range of 2-4) were found within the 30 by 30 m, suggesting that when a single point reading for this variable is selected for the validation of either remote sensing data or water-energy models, errors may occur. The results confirmed that in this landscape homogeneous 30 by 30 m plots can be used as landscape spatial units for soil moisture and ground temperature studies. Under this landscape conditions small plots can account for local expressions of environmental processes, decreasing the errors and uncertainties in remote sensing estimates caused by landscape heterogeneity.

The Predictive Factors for Diabetic Remission in Chinese Patients with BMI > 30 kg/m2 and BMI < 30 kg/m2 Are Different.

PubMed

Liang, Hui; Cao, Qing; Liu, Huan; Guan, Wei; Wong, Claudia; Tong, Daniel

2018-01-15

Roux-en-Y gastric bypass has been proven to be beneficial for patients with obesity and type 2 diabetes mellitus (T2DM). In less-obese patient (BMI 30-35 kg/m 2 ), surgical treatment is indicated when medication fails to control the T2DM. Asian develops diabetes at a lower BMI. For lower-BMI patients, the rate of diabetes amelioration varies significantly with patients of higher BMI after surgical treatment. The factors that contribute to the post-operative diabetes response rate in lower-BMI patients have not been elucidated. Between 2010 and 2014, a total of 144 patients who underwent gastric bypass for the treatment of T2DM were included for study. Patients were divided into two groups for subgroup analysis, namely BMI > 30 kg/m 2 and BMI < 30 kg/m 2 . Factors affecting the remission rate were examined. Of the studied patients, the DM remission rate for the high-BMI group (BMI > 30 kg/m 2 ) was 80% (n = 90) whereas for the lower BMI (BMI < 30 kg/m 2 ) was 50% (n = 54), p < 0.001. For high-BMI group, low HbA1c and high fasting C-peptide are predictive factors whereas for lower-BMI group, along with elevated C-peptide level, disease duration is the positive predictive factor for DM remission. Patients with BMI > 30 kg/m 2 and those with BMI < 30 kg/m 2 have distinct remission predicting factors. Low HbA1c is a predictor of remission in low-high-BMI patients while duration of diabetes is for high-low-BMI patients. C-peptide is a predictor of remission in both groups. Further large-scale studies are required to define the predictors of diabetes remission after gastric bypass in low- and high-BMI patients.

Relationship between liver injury and serum cytokeratin 18 levels in asymptomatic hepatitis B virus carriers and in patients with chronic hepatitis B infection.

PubMed

Balkan, Ayhan; Yılmaz, Nimet; Balkan, Yasemin; Koruk, Irfan; Örkmez, Mustafa; Aydınlı, Musa; Koruk, Mehmet

2017-06-01

Apoptosis represents a well-known mechanism of cell death involved in most chronic liver injuries. Our aim was to investigate the serum fragment level of cytokeratin 18 (CK18), M30, in asymptomatic hepatitis B virus (HBV) carriers and patients with chronic hepatitis B (CHB) and to evaluate the relationship between serum M30 levels and the severity of hepatic injury. Asymptomatic HBV carriers (n=169), patients with CHB (n=100), and healthy control subjects (n=43) were enrolled in the study. Serum CK18 (M30) levels were analysed in all subjects. Liver biopsy for histopathological assessment was performed in asymptomatic HBV carriers and in patients with CHB infection. Serum CK18 (M30) levels were significantly higher in asymptomatic HBV carriers (198.77±77.62U/L) than in healthy control subjects (146.92±40.18U/L). Patients with CHB (283.02±147.45U/L) had significantly higher CK18 (M30) levels than asymptomatic HBV carriers (p=0.001). The diagnostic efficacy of CK18 (M30) levels in distinguishing patients with HBeAg-negative CHB from asymptomatic HBV carriers was found to be moderate (c-statistics: 0.695), and the diagnostic cut-off value of CK18 (M30) was 262U/L (specificity: 85%, sensitivity: 48%, positive likelihood ratio: 3.35, and negative likelihood ratio: 0.60). There was a positive correlation between serum CK18 (M30) levels and histological activity index scores in asymptomatic HBV carriers and patients with CHB. Serum CK18 (M30) levels may be a valuable indicator in distinguishing asymptomatic HBV carriers from patients with HBeAg-negative CHB when considered together with ALT and HBV-DNA levels. Copyright © 2017 Pan-Arab Association of Gastroenterology. Published by Elsevier B.V. All rights reserved.

Long-term Treatment with Finasteride Resulted in a Significant Improvement Relative to Placebo in Clinical Progression of Benign Prostatic Hyperplasia (BPH) in Men with Enlarged Prostates (≥30 mL), But Not in Those with Smaller Prostates (<30 mL): Data from the Medical Therapy of Prostatic Symptoms (MTOPS) Trial

PubMed Central

Kaplan, Steven A.; Lee, Jeannette Y.; Meehan, Alan G.; Kusek, John W.

2013-01-01

Purpose This post hoc analysis of the Medical Therapy of Prostatic Symptoms (MTOPS) trial examined the effect of finasteride alone compared to placebo on clinical progression of benign prostatic hyperplasia (BPH) in men with baseline prostate volume (PV) <30 mL and ≥30 mL. Materials and Methods Men were randomized to placebo (n=737), doxazosin alone (4 to 8 mg) (n=756), finasteride alone (5 mg) (n=768), or doxazosin plus finasteride (n=786) (average duration of follow-up was 4.5 yrs); ~50% of patients had a baseline PV ≥30 mL. The present analysis was based on the finasteride alone and placebo arms only and included patients for whom baseline and end of study data were available. We examined the effect of treatment on the cumulative percentage of men who did not experience clinical progression of BPH by study end. Results In men with baseline PV ≥30 mL, treatment with finasteride produced a significant (p<0.001) increase relative to placebo in the cumulative percentage of patients who did not experience clinical progression of BPH (finasteride, 88.1%, versus placebo, 77.8%). There was no significant (p=0.441) between-group difference in men with baseline PV <30 mL (91.4% versus 89.1%, respectively). Conclusions Long-term treatment with finasteride led to a significant beneficial effect compared to placebo on clinical progression of BPH in LUTS patients with enlarged prostates (baseline PV ≥30 mL). Finasteride had no significant effect, compared to placebo on clinical progression of BPH in LUTS patients with smaller prostates (baseline PV <30 mL). PMID:21334655

Electronic Tongue Response to Chemicals in Orange Juice that Change Concentration in Relation to Harvest Maturity and Citrus Greening or Huanglongbing (HLB) Disease

PubMed Central

Raithore, Smita; Bai, Jinhe; Plotto, Anne; Manthey, John; Irey, Mike; Baldwin, Elizabeth

2015-01-01

In an earlier study, an electronic tongue system (e-tongue) has been used to differentiate between orange juice made from healthy fruit and from fruit affected by the citrus greening or Huanglongbing (HLB) disease. This study investigated the reaction of an e-tongue system to the main chemicals in orange juice that impact flavor and health benefits and are also impacted by HLB. Orange juice was spiked with sucrose (0.2–5.0 g/100 mL), citric acid (0.1%–3.0% g/100 mL) and potassium chloride (0.1–3.0 g/100 mL) as well as the secondary metabolites nomilin (1–30 µg/mL), limonin (1–30 µg/mL), limonin glucoside (30–200 µg/mL), hesperidin (30–400 µg/mL) and hesperetin (30–400 µg/mL). Performance of Alpha MOS sensor sets #1 (pharmaceutical) and #5 (food) were compared for the same samples, with sensor set #1 generally giving better separation than sensor set #5 for sucrose, sensor set #5 giving better separation for nomilin and limonin, both sets being efficient at separating citric acid, potassium chloride, hesperitin and limonin glucoside, and neither set discriminating hesperidin efficiently. Orange juice made from fruit over the harvest season and from fruit harvested from healthy or HLB-affected trees were separated by harvest maturity, disease state and disease severity. PMID:26633411

33 CFR 117.736 - Oceanport Creek.

Code of Federal Regulations, 2011 CFR

2011-07-01

... May 15 through September 15 between 5 a.m. and 9 p.m.; except that, the drawspan need not open 6 a.m. to 7:45 a.m. and 5:30 p.m. to 7:30 p.m on weekdays, excluding all federal holidays except for Martin... between 9 p.m. and 5 a.m., and from September 16 through May 14; except that, the drawspan need not be...

33 CFR 117.736 - Oceanport Creek.

Code of Federal Regulations, 2012 CFR

2012-07-01

... May 15 through September 15 between 5 a.m. and 9 p.m.; except that, the drawspan need not open 6 a.m. to 7:45 a.m. and 5:30 p.m. to 7:30 p.m on weekdays, excluding all federal holidays except for Martin... between 9 p.m. and 5 a.m., and from September 16 through May 14; except that, the drawspan need not be...

33 CFR 117.736 - Oceanport Creek.

Code of Federal Regulations, 2013 CFR

2013-07-01

... May 15 through September 15 between 5 a.m. and 9 p.m.; except that, the drawspan need not open 6 a.m. to 7:45 a.m. and 5:30 p.m. to 7:30 p.m on weekdays, excluding all federal holidays except for Martin... between 9 p.m. and 5 a.m., and from September 16 through May 14; except that, the drawspan need not be...

33 CFR 117.736 - Oceanport Creek.

Code of Federal Regulations, 2014 CFR

2014-07-01

... May 15 through September 15 between 5 a.m. and 9 p.m.; except that, the drawspan need not open 6 a.m. to 7:45 a.m. and 5:30 p.m. to 7:30 p.m on weekdays, excluding all federal holidays except for Martin... between 9 p.m. and 5 a.m., and from September 16 through May 14; except that, the drawspan need not be...

36 CFR 1253.7 - Regional Archives.

Code of Federal Regulations, 2010 CFR

2010-07-01

.... Hours are Monday, Tuesday, Wednesday, Friday, 7 a.m. to 4:30 p.m., Thursday, 7 a.m. to 9 p.m., and some... located at 10 Conte Drive, Pittsfield, MA 01201-8230. The hours are 8 a.m. to 4:30 p.m., Monday through... 7358 S. Pulaski Rd., Chicago, IL 60629-5898. The hours are 8 a.m. to 4:15 p.m., Monday through Friday...

33 CFR 117.221 - Saugatuck River.

Code of Federal Regulations, 2013 CFR

2013-07-01

.... (3) From June 1-September 30, open on signal 5 a.m.-9 p.m., except as provided in paragraph (b)(1)(i...) Weekdays from 7 a.m. to 8:10 a.m. and 5:30 p.m. to 7 p.m. except on Federal holidays; (ii) From 9 p.m. to 5... and Federal holidays 7 a.m.-4 p.m.; (iii) If at least eight hours notice is given: daily, from 5 a.m...

33 CFR 117.221 - Saugatuck River.

Code of Federal Regulations, 2012 CFR

2012-07-01

.... (3) From June 1-September 30, open on signal 5 a.m.-9 p.m., except as provided in paragraph (b)(1)(i...) Weekdays from 7 a.m. to 8:10 a.m. and 5:30 p.m. to 7 p.m. except on Federal holidays; (ii) From 9 p.m. to 5... and Federal holidays 7 a.m.-4 p.m.; (iii) If at least eight hours notice is given: daily, from 5 a.m...

33 CFR 117.221 - Saugatuck River.

Code of Federal Regulations, 2010 CFR

2010-07-01

.... (3) From June 1-September 30, open on signal 5 a.m.-9 p.m., except as provided in paragraph (b)(1)(i...) Weekdays from 7 a.m. to 8:10 a.m. and 5:30 p.m. to 7 p.m. except on Federal holidays; (ii) From 9 p.m. to 5... and Federal holidays 7 a.m.-4 p.m.; (iii) If at least eight hours notice is given: daily, from 5 a.m...

33 CFR 117.221 - Saugatuck River.

Code of Federal Regulations, 2011 CFR

2011-07-01

.... (3) From June 1-September 30, open on signal 5 a.m.-9 p.m., except as provided in paragraph (b)(1)(i...) Weekdays from 7 a.m. to 8:10 a.m. and 5:30 p.m. to 7 p.m. except on Federal holidays; (ii) From 9 p.m. to 5... and Federal holidays 7 a.m.-4 p.m.; (iii) If at least eight hours notice is given: daily, from 5 a.m...

33 CFR 117.221 - Saugatuck River.

Code of Federal Regulations, 2014 CFR

2014-07-01

.... (3) From June 1-September 30, open on signal 5 a.m.-9 p.m., except as provided in paragraph (b)(1)(i...) Weekdays from 7 a.m. to 8:10 a.m. and 5:30 p.m. to 7 p.m. except on Federal holidays; (ii) From 9 p.m. to 5... and Federal holidays 7 a.m.-4 p.m.; (iii) If at least eight hours notice is given: daily, from 5 a.m...

75 FR 5602 - Center for Scientific Review; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-03

... personal privacy. Name of Committee: Genes, Genomes, and Genetics Integrated Review Group; Ethical, Legal, and Social Implications of Human Genetics Study Section. Date: February 23, 2010. Time: 8:30 a.m. to 4... Conflict: Cancer Genetics and Others. Date: February 26, 2010. Time: 10:30 a.m. to 1:30 p.m. Agenda: To...

75 FR 67149 - Board of Governors; Sunshine Act Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-01

... POSTAL SERVICE Board of Governors; Sunshine Act Meeting DATES AND TIMES: Thursday, November 11, 2010, at 10:30 a.m.; and Friday, November 12, at 8:30 a.m. and 11:30 a.m. PLACE: Washington, DC, at U.S. Postal Service Headquarters, 475 L'Enfant Plaza, SW., in the Benjamin Franklin Room. STATUS: Thursday...

77 FR 2734 - National Advisory Council on Migrant Health; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-19

... Advisory Council on Migrant Health. Dates and Times: February 7, 2012, 8:30 a.m. to 5 p.m.; February 8, 2012, 8:30 a.m. to 5 p.m. Place: Crystal Gateway Marriott Hotel, 1700 Jefferson Davis Highway...

33 CFR 117.619 - Taunton River.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Brightman Street (Route-6) Bridge at mile 1.8, between Fall River and Somerset, shall operate as follows: (b) The draw shall open on signal between 5 a.m. and 9 p.m., daily. From 9 p.m. through 5 a.m. the draw... craft from 7 a.m. to 9:30 a.m. and from 4 p.m. to 6:30 p.m., Monday through Friday, except holidays. The...

33 CFR 117.619 - Taunton River.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Brightman Street (Route-6) Bridge at mile 1.8, between Fall River and Somerset, shall operate as follows: (b) The draw shall open on signal between 5 a.m. and 9 p.m., daily. From 9 p.m. through 5 a.m. the draw... craft from 7 a.m. to 9:30 a.m. and from 4 p.m. to 6:30 p.m., Monday through Friday, except holidays. The...

33 CFR 117.619 - Taunton River.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Brightman Street (Route-6) Bridge at mile 1.8, between Fall River and Somerset, shall operate as follows: (b) The draw shall open on signal between 5 a.m. and 9 p.m., daily. From 9 p.m. through 5 a.m. the draw... craft from 7 a.m. to 9:30 a.m. and from 4 p.m. to 6:30 p.m., Monday through Friday, except holidays. The...

33 CFR 117.1041 - Duwamish Waterway.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., mile 2.5, need not be opened for the passage of vessels from 6 a.m. to 9 a.m. and from 3 p.m. to 6 p.m... passage of vessels from 6:30 a.m. to 8:00 a.m. and 3:30 p.m. to 5:00 p.m., Monday through Friday, except... of each bridge across the Duwamish Waterway shall open on signal, except as follows: (1) From Monday...

33 CFR 117.1041 - Duwamish Waterway.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., mile 2.5, need not be opened for the passage of vessels from 6 a.m. to 9 a.m. and from 3 p.m. to 6 p.m... passage of vessels from 6:30 a.m. to 8:00 a.m. and 3:30 p.m. to 5:00 p.m., Monday through Friday, except... of each bridge across the Duwamish Waterway shall open on signal, except as follows: (1) From Monday...

Effect of the 2.0 mg/m3 coal mine dust standard on underground environmental dust levels.

PubMed

Parobeck

1975-08-01

The 1969 Federal Coal Mine Health and Safety Act established environmental dust standards for underground coal mines. The Act requires that the average concentration of respirable dust in the active workings of a mine be maintained at or below 3.0 mg/m3; and, that effective December 30, 1972, the 3.0 mg/m3 standard be reduced to 2.0 mg/m3. This paper discusses the current status of dust levels in our underground coal mines, the effect of the 2.0 mg/m3 standard on underground dust levels, and associates the current levels with specific operations and occupations. The comparison is made between current levels and those existing prior to December 30, 1972.

Demonstration/Validation of the Snap Sampler

DTIC Science & Technology

2011-06-01

0 10 20 30 40 50 60 Low Flow (mg/L) Sn ap S am pl er (m g/ L) Total Iron 0 5 10 15 20 25 0 5 10 15 20 25 Low Flow (mg/L) Sn ap S am pl er...concentrations of Mg. 0 10 20 30 40 50 60 0 10 20 30 40 50 60 Total Mg (mg/L) Fi lte re d M g (m g/ L) Figure 5 -4. Plot comparing concentra- tions...TECHNOLOGY .................... 5   3.0  DEMONSTRATION DESIGN

Monensin and a blend of castor oil and cashew nut shell liquid used in a high-concentrate diet abruptly fed to Nellore cattle.

PubMed

Zotti, C A; Silva, A P; Carvalho, R; Marino, C T; Rodrigues, P H M; Silva, L F P; McAllister, T A; Leme, P R

2017-09-01

Monensin and functional oils (FO) were supplemented to a high-concentrate diet abruptly fed to 12 ruminally cannulated Zebu steers to study their effects on rumen fermentation, blood metabolites, and , , and relative population. A randomized complete block design with repeated measures over time within 2 experimental periods of 21 d each was used. Treatments were a control (CTR; with no additives), FO (included at 400 mg/kg), and monensin included at 30 mg/kg (M30) or 40 mg/kg (M40). All steers were fed the same high-concentrate basal diet, which consisted of 92.25% concentrate. The first 60 h after transition showed a treatment and hour interaction for ruminal propionate proportion ( = 0.028), and no change in acetate molar proportion ( = 0.633), rumen pH ( = 0.370), and time the rumen pH remained below 5.6 ( = 0.242) were observed. The acetate:propionate ratio decreased ( = 0.020) when monensin was fed in both concentrations (2.30 for the M30 treatment and 2.32 for the M40 treatment) compared with when the CTR was fed (2.85), without being different when the FO (2.71) treatment was fed. Only the M30 treatment did not show pH below 5.2 (P=0.047) over the 60 h after the abrupt transition. Within the entire period, DMI ( = 0.008) and mean ruminal pH ( = 0.040) as well as molar proportions of propionate ( = 0.034) and valerate ( = 0.031) had significant interactions between treatment and day. Total VFA concentration was greater ( = 0.017) for the M30 (117.36 m) and CTR treatments (115.77 m) compared with the M40 treatment (105.02 m), without being different for the FO treatment (111.55 m). Treatments did not change feed behavior parameters. Blood HCO ( = 0.006) and total carbon dioxide ( = 0.003) were greater for the M30 (27.8 and 29.3 mmol/L, respectively) and FO treatments (28.3 and 29.7 mmol/L, respectively) compared with the CTR treatment (25.7 and 26.9 mmol/L, respectively). ( < 0.0001) and ( < 0.0001) decreased their population throughout days, whereas ( = 0.026) increased its population. Independent of ciliated protozoa genera, the greatest ( < 0.0001) protozoa counts were observed for the CTR treatment (52.7 × 10/mL), intermediate for the FO treatment (35.3 x10/mL), and least for steers fed monensin in both concentrations (15 × 10/mL for the M30 treatment and 14 × 10/mL for the M40 treatment). Feed additives had different effects to reduce the subacute acidosis. The use of the FO and M40 treatments did not change most of the rumen fermentation variables, especially in the first week after abrupt transition, when the M30 treatment provided higher protection against acidosis.

33 CFR 117.541 - Baltimore Harbor-Patapsco River.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Patapsco River at Baltimore, shall open on signal from 5 a.m. to 6:30 a.m., 9:30 a.m. to 4 p.m., and 6 p.m... during this period. When a vessel desires to pass the draw from 9 p.m. to 5 a.m., notice shall be given... residence after 9 p.m. If the notice is given from 5 a.m. to 9 p.m. or if at least one half hour has elapsed...

33 CFR 117.541 - Baltimore Harbor-Patapsco River.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Patapsco River at Baltimore, shall open on signal from 5 a.m. to 6:30 a.m., 9:30 a.m. to 4 p.m., and 6 p.m... during this period. When a vessel desires to pass the draw from 9 p.m. to 5 a.m., notice shall be given... residence after 9 p.m. If the notice is given from 5 a.m. to 9 p.m. or if at least one half hour has elapsed...

76 FR 63666 - Sunshine Act Meeting of the National Museum and Library Services Board

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-13

... Library Services Board AGENCY: Institute of Museum and Library Services (IMLS), NFAH. ACTION: Notice of... Library Services Board. This notice also describes the function of the Board. Notice of the meeting is... Library Service Board Meeting: 9:30 a.m.-11:30 a.m. Executive Session (Closed to the Public) 1:30 p.m.-4...

75 FR 1088 - Board of Governors; Sunshine Act Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-08

... POSTAL SERVICE Board of Governors; Sunshine Act Meeting TIMES AND DATES: 3:30 p.m., Monday, January 11, 2010; and 7:30 a.m., Tuesday, January 12, 2010. PLACE: Newport Beach, California, at the Fairmont Hotel, 4500 MacArthur Boulevard. STATUS: (Closed) MATTERS TO BE CONSIDERED: Monday, January 11, at 3:30 p.m. (Closed) 1. Strategic Issues. 2....

A saxitoxin-binding aptamer with higher affinity and inhibitory activity optimized by rational site-directed mutagenesis and truncation.

PubMed

Zheng, X; Hu, B; Gao, S X; Liu, D J; Sun, M J; Jiao, B H; Wang, L H

2015-07-01

Saxitoxin (STX), a member of the family of paralytic shellfish poisoning toxins, poses toxicological and ecotoxicological risks. To develop an analytical recognition element for STX, a DNA aptamer (APT(STX1)) was previously discovered via an iterative process known as Systematic Evolution of Ligands by Exponential Enrichment (SELEX) by Handy et al. Our study focused on generating an improved aptamer based on APT(STX1) through rational site-directed mutation and truncation. In this study, we generated the aptamer, M-30f, with a 30-fold higher affinity for STX compared with APT(STX1). The Kd value for M-30f was 133 nM, which was calculated by Bio-Layer Interferometry. After optimization, we detected and compared the interaction of STX with aptamers (APT(STX1) or M-30f) through several techniques (ELISA, cell bioassay, and mouse bioassay). Both aptamers' STX-binding ability was demonstrated in all three methods. Moreover, M-30f performs better than its parent sequence with higher suppressive activity against STX. As a molecular recognition element, M-30f has good prospects for practical application. Copyright © 2015 Elsevier Ltd. All rights reserved.

78 FR 69701 - Center for Scientific Review; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-20

... Panel; PAR 13-061: Tuberculosis. Date: December 13, 2013. Time: 10:00 a.m. to 6:30 p.m. Agenda: To... Emphasis Panel; Tuberculosis. Date: December 13, 2013. Time: 3:30 p.m. to 5:00 p.m. Agenda: To review and...

77 FR 21592 - Proposal Review Panel for Materials Research; Notice of Meeting: Correction

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-10

... 19362, column 3, line 3, please replace: ``7 p.m.-8:30p.m. Open-Poster Session'' with ``7 p.m.-10:30p.m. Open-Poster Session''. Dated: April 5, 2012. Susanne Bolton, Committee Management Officer. [FR Doc...

76 FR 69745 - Center for Scientific Review; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-09

...: Cell Biology. Date: December 8-9, 2011. Time: 8 a.m. to 5:30 p.m. Agenda: To review and evaluate grant..., Program Project: Developmental Pharmacology. Date: December 14-15, 2011. Time: 8 a.m. to 5:30 p.m. Agenda...

Does pretransplant soluble CD30 serum concentration affect deceased-donor kidney graft function 3 years after transplantation?

PubMed

Kovac, J; Arnol, M; Vidan-Jeras, B; Bren, A F; Kandus, A

2008-06-01

Elevated serum concentrations of soluble CD30 molecule (sCD30) have been related to acute cellular rejection and poor graft outcomes in kidney transplantation. This historical cohort study investigated the association of pretransplant sCD30 serum concentrations with kidney graft function expressed as estimated glomerular filtration rate (GFR) at 3 years after transplantation. Pretransplant sera from 176 adult deceased-donor kidney graft recipients were tested for sCD30 content using a commercially available automated enzyme-linked immunosorbent assay. The immunosuppression consisted of induction therapy with monoclonal anti-CD25 antibodies and a maintenance regimen of cyclosporine (CsA)-based therapy. GFR was estimated (eGFR) by the four-variable Modification of Diet in Renal Disease (MDRD) Study equation. According to the distribution of pretransplant sCD30 levels (median 66.7 U/mL; interquartile range, 46.6 to 98.6 U/mL), a concentration of 66 U/mL or higher was defined as high (n = 89) and below 66 U/mL as low (n = 87). Three years after transplantation, eGFR was not significantly different among recipients in high versus low sCD30 groups (69 +/- 23 mL/min/1.73m2 vs 66 +/- 21 mL/min/1.73m2; P = .327) and there was no correlation between eGFR and pretransplant sCD30 levels (r2 = 0.001; P = .73). Upon multivariate regression analysis, donor age, recipient body mass index at transplantation, and acute rejection episodes were independent variables affecting eGFR at 3 years after transplantation. This study showed that pretransplant sCD30 serum concentrations were not associated with deceased-donor kidney graft function at 3 years after transplantation. The immunosuppression with anti-CD25 antibodies and a triple CsA-based maintenance regimen could possibly be decisive for our findings.

Evaluation of serum sCD30 in renal transplantation patients with and without acute rejection.

PubMed

Cervelli, C; Fontecchio, G; Scimitarra, M; Azzarone, R; Famulari, A; Pisani, F; Battistoni, C; Di Iulio, B; Fracassi, D; Scarnecchia, M A; Papola, F

2009-05-01

Despite new immunosuppressive approaches, acute rejection episodes (ARE) are still a major cause of early kidney dysfunction with a negative impact on long-term allograft survival. Noninvasive markers able to identify renal ARE earlier than creatinine measurement include sCD30. We sought to establish whether circulating levels of sCD30 in pretransplantation and posttransplantation periods were of clinical relevance to avoid graft damage. Quantitative detection of serum sCD30 was performed using an enzyme-linked immunosorbent assay. Our results demonstrated that the mean concentrations of sCD30 were significantly higher in the sera of renal transplant recipients with ARE (30.04 U/mL) and in uremic patients on the waiting list (37.7 U/mL) compared with healthy controls (HC; 9.44 U/mL), but not nonrejecting patients (12.01 U/mL). Statistical analysis revealed a strong association between high sCD30 levels in posttransplantation sera and ARE risk. This study suggested that sCD30 levels were a reliable predictor of ARE among deceased-donor kidney recipients.

Estrogen Enhances Linkage in the Vascular Endothelial Calmodulin Network via a Feedforward Mechanism at the G Protein-coupled Estrogen Receptor 1*

PubMed Central

Tran, Quang-Kim; Firkins, Rachel; Giles, Jennifer; Francis, Sarah; Matnishian, Vahe; Tran, Phuong; VerMeer, Mark; Jasurda, Jake; Burgard, Michelle Ann; Gebert-Oberle, Briana

2016-01-01

Estrogen exerts many effects on the vascular endothelium. Calmodulin (CaM) is the transducer of Ca2+ signals and is a limiting factor in cardiovascular tissues. It is unknown whether and how estrogen modifies endothelial functions via the network of CaM-dependent proteins. Here we show that 17β-estradiol (E2) up-regulates total CaM level in endothelial cells. Concurrent measurement of Ca2+ and Ca2+-CaM indicated that E2 also increases free Ca2+-CaM. Pharmacological studies, gene silencing, and receptor expression-specific cell studies indicated that the G protein-coupled estrogen receptor 1 (GPER/GPR30) mediates these effects via transactivation of EGFR and subsequent MAPK activation. The outcomes were then examined on four distinct members of the intracellular CaM target network, including GPER/GPR30 itself and estrogen receptor α, the plasma membrane Ca2+-ATPase (PMCA), and endothelial nitric-oxide synthase (eNOS). E2 substantially increases CaM binding to estrogen receptor α and GPER/GPR30. Mutations that reduced CaM binding to GPER/GPR30 in separate binding domains do not affect GPER/GPR30-Gβγ preassociation but decrease GPER/GPR30-mediated ERK1/2 phosphorylation. E2 increases CaM-PMCA association, but the expected stimulation of Ca2+ efflux is reversed by E2-stimulated tyrosine phosphorylation of PMCA. These effects sustain Ca2+ signals and promote Ca2+-dependent CaM interactions with other CaM targets. Consequently, E2 doubles CaM-eNOS interaction and also promotes dual phosphorylation of eNOS at Ser-617 and Ser-1179. Calculations using in-cell and in vitro data revealed substantial individual and combined contribution of these effects to total eNOS activity. Taken together, E2 generates a feedforward loop via GPER/GPR30, which enhances Ca2+/CaM signals and functional linkage in the endothelial CaM target network. PMID:26987903

Estrogen Enhances Linkage in the Vascular Endothelial Calmodulin Network via a Feedforward Mechanism at the G Protein-coupled Estrogen Receptor 1.

PubMed

Tran, Quang-Kim; Firkins, Rachel; Giles, Jennifer; Francis, Sarah; Matnishian, Vahe; Tran, Phuong; VerMeer, Mark; Jasurda, Jake; Burgard, Michelle Ann; Gebert-Oberle, Briana

2016-05-13

Estrogen exerts many effects on the vascular endothelium. Calmodulin (CaM) is the transducer of Ca(2+) signals and is a limiting factor in cardiovascular tissues. It is unknown whether and how estrogen modifies endothelial functions via the network of CaM-dependent proteins. Here we show that 17β-estradiol (E2) up-regulates total CaM level in endothelial cells. Concurrent measurement of Ca(2+) and Ca(2+)-CaM indicated that E2 also increases free Ca(2+)-CaM. Pharmacological studies, gene silencing, and receptor expression-specific cell studies indicated that the G protein-coupled estrogen receptor 1 (GPER/GPR30) mediates these effects via transactivation of EGFR and subsequent MAPK activation. The outcomes were then examined on four distinct members of the intracellular CaM target network, including GPER/GPR30 itself and estrogen receptor α, the plasma membrane Ca(2+)-ATPase (PMCA), and endothelial nitric-oxide synthase (eNOS). E2 substantially increases CaM binding to estrogen receptor α and GPER/GPR30. Mutations that reduced CaM binding to GPER/GPR30 in separate binding domains do not affect GPER/GPR30-Gβγ preassociation but decrease GPER/GPR30-mediated ERK1/2 phosphorylation. E2 increases CaM-PMCA association, but the expected stimulation of Ca(2+) efflux is reversed by E2-stimulated tyrosine phosphorylation of PMCA. These effects sustain Ca(2+) signals and promote Ca(2+)-dependent CaM interactions with other CaM targets. Consequently, E2 doubles CaM-eNOS interaction and also promotes dual phosphorylation of eNOS at Ser-617 and Ser-1179. Calculations using in-cell and in vitro data revealed substantial individual and combined contribution of these effects to total eNOS activity. Taken together, E2 generates a feedforward loop via GPER/GPR30, which enhances Ca(2+)/CaM signals and functional linkage in the endothelial CaM target network. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

MiR-30e suppresses proliferation of hepatoma cells via targeting prolyl 4-hydroxylase subunit alpha-1 (P4HA1) mRNA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Feng, Guoxing; Shi, Hui; Li, Jiong

Aberrant microRNA expression has been shown to be characteristic of many cancers. It has been reported that the expression levels of miR-30e are decreased in liver cancer tissues. However, the role of miR-30e in hepatocellular carcinoma remains poorly understood. In the present study, we investigated the significance of miR-30e in hepatocarcinogenesis. Bioinformatics analysis reveals a putative target site of miR-30e in the 3′-untranslated region (3′UTR) of prolyl 4-hydroxylase subunit alpha-1 (P4HA1) mRNA. Moreover, luciferase reporter gene assays verified that miR-30e directly targeted 3′UTR of P4HA1 mRNA. Then, we demonstrated that miR-30e was able to reduce the expression of P4HA1 atmore » the levels of mRNA and protein using reverse transcription-polymerase chain reaction and Western blot analysis. Enforced expression of miR-30e suppressed proliferation of HepG2 cells by 5-ethynyl-2-deoxyuridine (EdU) assay and reduced colony formation of these cells by colony formation analysis. Conversely, anti-miR-30e enhanced the proliferation of hepatoma cells in vitro. Interestingly, the ectopic expression of P4HA1 could efficiently rescue the inhibition of cell proliferation mediated by miR-30e in HepG2 cells. Meanwhile, silencing of P4HA1 abolished the anti-miR-30e-induced proliferation of cells. Clinically, quantitative real-time PCR showed that miR-30e was down-regulated in liver tumor tissues relative to their peritumor tissues. The expression levels of miR-30e were negatively correlated to those of P4HA1 mRNA in clinical liver tumor tissues. Thus, we conclude that miR-30e suppresses proliferation of hepatoma cells through targeting P4HA1 mRNA. Our finding provides new insights into the mechanism of hepatocarcinogenesis. - Highlights: • P4HA1 is a novel target gene of miR-30e. • P4HA1 is increased in clinical HCC tissues. • MiR-30e is negatively correlated with P4HA1 in clinical HCC tissues. • MiR-30e suppresses the proliferation of HCC cells through targeting P4HA1 mRNA.« less

High pre-transplant soluble CD30 levels are predictive of the grade of rejection.

PubMed

Rajakariar, Ravindra; Jivanji, Naina; Varagunam, Mira; Rafiq, Mohammad; Gupta, Arun; Sheaff, Michael; Sinnott, Paul; Yaqoob, M M

2005-08-01

In renal transplantation, serum soluble CD30 (sCD30) levels in graft recipients are associated with increased rejection and graft loss. We investigated whether pre-transplant sCD30 concentrations are predictive of the grade of rejection. Pre-transplant sera of 51 patients with tubulointerstitial rejection (TIR), 16 patients with vascular rejection (VR) and an age-matched control group of 41 patients with no rejection (NR) were analyzed for sCD30. The transplant biopsies were immunostained for C4d. The median sCD30 level was significantly elevated in the group with VR (248 Units (U)/mL, range: 92-802) when compared with TIR (103 U/mL, range: 36-309, p<0.001) and NR (179 U/mL, range: 70-343, p<0.03). Moreover, patients with TIR had significantly lower sCD30 levels compared to NR. Based on C4d staining, a TH2 driven process, the median sCD30 levels were significantly raised in C4d+ patients compared with C4d- group (177 U/mL vs. 120 U/mL, p<0.05). sCD30 levels measured at time of transplantation correlate with the grade of rejection. High pre-transplant levels are associated with antibody-mediated rejection which carries a poorer prognosis. sCD30 could be another tool to assess immunological risk prior to transplantation and enable a patient centered approach to immunosuppression.

Time Dependent Holographic Interferometry and Finite-Element Analysis of Heat Transfer Within a Rectangular Enclosure

DTIC Science & Technology

1976-09-01

describing the system are correctly assembled, a library subroutine (LEQT2F) functioning as a linear equation solver is called and the desired nodal... mooc ^30 •»-< * #00 + O00ɜ-tH Q(M I > •.-4(M-M- +30 ^f0*O I >o • -t-W-QOO 30 t^*Q(Mw O tO^ I o«o»-< •^ + 00 I fH (M(M3 -*>* (M +«o o fOOO *3Q • — » aoo...Documentation Center 2 Cameron Station Alexandria, Virginia 22314 Library , Code 0212 2 Naval Postgraduate School Monterey, California 93940 3

Impairment of autophagy by TTR V30M aggregates: in vivo reversal by TUDCA and curcumin.

PubMed

Teixeira, Cristina A; Almeida, Maria do Rosário; Saraiva, Maria João

2016-09-01

Transthyretin (TTR)-related amyloidoses are diseases characterized by extracellular deposition of amyloid fibrils and aggregates in tissues composed of insoluble misfolded TTR that becomes toxic. Previous studies have demonstrated the ability of small compounds in preventing and reversing TTR V30M deposition in transgenic mice gastrointestinal (GI) tract as well as lowering biomarkers associated with cellular stress and apoptotic mechanisms. In the present study we aimed to study TTR V30M aggregates effect in autophagy, a cellular mechanism crucial for cell survival that has been implicated in the development of several neurodegenerative diseases. We were able to demonstrate in cell culture that TTR V30M aggregates cause a partial impairment of the autophagic machinery as shown by p62 accumulation, whereas early steps of the autophagic flux remain unaffected as shown by autophagosome number evaluation and LC3 turnover assay. Our studies performed in TTR V30M transgenic animals demonstrated that tauroursodeoxycholic acid (TUDCA) and curcumin effectively reverse p62 accumulation in the GI tract pointing to the ability of both compounds to modulate autophagy additionally to mitigate apoptosis. Overall, our in vitro and in vivo studies establish an association between TTR V30M aggregates and autophagy impairment and suggest the use of autophagy modulators as an additional and alternative therapeutic approach for the treatment of TTR V30M-related amyloidosis. © 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society.

33 CFR 117.273 - Canaveral Barge Canal.

Code of Federal Regulations, 2012 CFR

2012-07-01

....m. to 8:15 a.m. and from 3:10 p.m. to 5:59 p.m., Monday through Friday, except Federal holidays, the drawspan need not open. From 10:01 p.m. to 5:59 a.m., everyday, the drawspan must open on signal if at... Drawbridge, mile 5.5 at Port Canaveral, must open on signal; except that, from 6:30 a.m. to 8 a.m. and 3:30 p...

33 CFR 117.273 - Canaveral Barge Canal.

Code of Federal Regulations, 2010 CFR

2010-07-01

....m. to 8:15 a.m. and from 3:10 p.m. to 5:59 p.m., Monday through Friday, except Federal holidays, the drawspan need not open. From 10:01 p.m. to 5:59 a.m., everyday, the drawspan must open on signal if at... Drawbridge, mile 5.5 at Port Canaveral, must open on signal; except that, from 6:30 a.m. to 8 a.m. and 3:30 p...

33 CFR 117.273 - Canaveral Barge Canal.

Code of Federal Regulations, 2014 CFR

2014-07-01

....m. to 8:15 a.m. and from 3:10 p.m. to 5:59 p.m., Monday through Friday, except Federal holidays, the drawspan need not open. From 10:01 p.m. to 5:59 a.m., everyday, the drawspan must open on signal if at... Drawbridge, mile 5.5 at Port Canaveral, must open on signal; except that, from 6:30 a.m. to 8 a.m. and 3:30 p...

33 CFR 117.273 - Canaveral Barge Canal.

Code of Federal Regulations, 2011 CFR

2011-07-01

....m. to 8:15 a.m. and from 3:10 p.m. to 5:59 p.m., Monday through Friday, except Federal holidays, the drawspan need not open. From 10:01 p.m. to 5:59 a.m., everyday, the drawspan must open on signal if at... Drawbridge, mile 5.5 at Port Canaveral, must open on signal; except that, from 6:30 a.m. to 8 a.m. and 3:30 p...

33 CFR 117.273 - Canaveral Barge Canal.

Code of Federal Regulations, 2013 CFR

2013-07-01

....m. to 8:15 a.m. and from 3:10 p.m. to 5:59 p.m., Monday through Friday, except Federal holidays, the drawspan need not open. From 10:01 p.m. to 5:59 a.m., everyday, the drawspan must open on signal if at... Drawbridge, mile 5.5 at Port Canaveral, must open on signal; except that, from 6:30 a.m. to 8 a.m. and 3:30 p...

33 CFR 117.566 - Patapsco River-Middle Branch.

Code of Federal Regulations, 2014 CFR

2014-07-01

... River at Baltimore, will open on signal from 5 a.m. to 6:30 a.m., 9:30 a.m. to 4 p.m., and 6 p.m. to 9... this period. When a vessel desires to pass the draw from 9 p.m. to 5 a.m., notice will be given to the.... If the notice is given from 5 a.m. to 9 p.m. or if at least one half hour has elapsed since the...

33 CFR 117.566 - Patapsco River-Middle Branch.

Code of Federal Regulations, 2012 CFR

2012-07-01

... River at Baltimore, will open on signal from 5 a.m. to 6:30 a.m., 9:30 a.m. to 4 p.m., and 6 p.m. to 9... this period. When a vessel desires to pass the draw from 9 p.m. to 5 a.m., notice will be given to the.... If the notice is given from 5 a.m. to 9 p.m. or if at least one half hour has elapsed since the...

33 CFR 117.566 - Patapsco River-Middle Branch.

Code of Federal Regulations, 2013 CFR

2013-07-01

... River at Baltimore, will open on signal from 5 a.m. to 6:30 a.m., 9:30 a.m. to 4 p.m., and 6 p.m. to 9... this period. When a vessel desires to pass the draw from 9 p.m. to 5 a.m., notice will be given to the.... If the notice is given from 5 a.m. to 9 p.m. or if at least one half hour has elapsed since the...

Near-Surface Shear Wave Velocity Versus Depth Profiles, VS30, and NEHRP Classifications for 27 Sites in Puerto Rico

USGS Publications Warehouse

Odum, Jack K.; Williams, Robert A.; Stephenson, William J.; Worley, David M.; von Hillebrandt-Andrade, Christa; Asencio, Eugenio; Irizarry, Harold; Cameron, Antonio

2007-01-01

In 2004 and 2005 the Puerto Rico Seismic Network (PRSN), Puerto Rico Strong Motion Program (PRSMP) and the Geology Department at the University of Puerto Rico-Mayaguez (UPRM) collaborated with the U.S. Geological Survey to study near-surface shear-wave (Vs) and compressional-wave (Vp) velocities in and around major urban areas of Puerto Rico. Using noninvasive seismic refraction-reflection profiling techniques, we acquired velocities at 27 locations. Surveyed sites were predominantly selected on the premise that they were generally representative of near-surface materials associated with the primary geologic units located within the urbanized areas of Puerto Rico. Geologic units surveyed included Cretaceous intrusive and volcaniclastic bedrock, Tertiary sedimentary and volcanic units, and Quaternary unconsolidated eolian, fluvial, beach, and lagoon deposits. From the data we developed Vs and Vp depth versus velocity columns, calculated average Vs to 30-m depth (VS30), and derived NEHRP (National Earthquake Hazards Reduction Program) site classifications for all sites except one where results did not reach 30-m depth. The distribution of estimated NEHRP classes is as follows: three class 'E' (VS30 below 180 m/s), nine class 'D' (VS30 between 180 and 360 m/s), ten class 'C' (VS30 between 360 and 760 m/s), and four class 'B' (VS30 greater than 760 m/s). Results are being used to calibrate site response at seismograph stations and in the development of regional and local shakemap models for Puerto Rico.

75 FR 52357 - Center for Scientific Review; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-25

... Disorders of Aging Study Section. Date: September 30-October 1, 2010. Time: 8:30 a.m. to 6 p.m. Agenda: To..., Stress, and Health. Date: October 5, 2010. Time: 1 p.m. to 5 p.m. Agenda: To review and evaluate grant...

76 FR 29769 - National Advisory Council on the National Health Service Corps; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-23

...: June 22, 2011-8:30 a.m.-4:30 p.m. June 23, 2011--8 a.m.--12 p.m. Place: Saddlebrook Tampa, 5700...-based strategies for clinician retention, and a new communications tool for clinicians. A portion of the...

78 FR 2259 - President's Council of Advisors on Science and Technology (PCAST)

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-10

...'s Climate and Energy letter report. DATES: Thursday, January 24, 2013 from 5:00 p.m. to 5:30 p.m..., 2013 from 5:00 p.m. to 5:30 p.m. During the conference call, PCAST will discuss its Climate and Energy...

Characterization of vertical electric fields 500 m and 30 m from triggered lightning

NASA Astrophysics Data System (ADS)

Rubenstein, M.; Rachidi, F.; Uman, M. A.; Thottappillil, R.; Rakov, V. A.; Nucci, C. A.

1995-05-01

Vertical electric field waveforms of leader-return stroke sequences measured 500 m and 30 m from rocket-triggered lightning are presented. The 500-m data were recorded during the summer of 1986, the 30-m data during the summer of 1991, both at the NASA Kennedy Space Center, Florida. The 40 leader-return stroke field waveforms at 500 m and the 8 waveforms at 30 m all appear as asymmetrical V-shaped pulses, the bottom of the V being associated with the transition from the leader to the return stroke. Only two waveforms at 30 m were suitable for quantitative analysis. The widths of the V at half of peak value for these are 1.8 and 5.0 μs, while for the 500-m data they are 1 to 2 orders of magnitude greater, with a median value of 100 μs. Applying a widely used and simple leader model to the measured leader electric fields at 500 m, we infer, for the bottom kilometer or so of the leader channel, leader speeds between 2×106 and 2×107 m/s and leader charges per unit length of 0.02×10-3 to 0.08×10-3 C/m. From the two measured leader electric field changes at 30 m we infer, using the same leader model, for the bottom 100 meters or so of the leader channel, speeds of 3×107 and 1×107 m/s (the corresponding measured waveform half widths are 1.8 μs and 5.0 μs) and charges per unit length of 0.14×10-3 and 0.02×10-3 C/m (the corresponding measured leader field changes are 81 kV/m and 12 kV/m). The corresponding measured return stroke peak currents for the above two cases are 40 kA and 7 kA, respectively. A positive correlation is observed between the magnitude of the leader field change at 500 m and the ensuing return stroke current peak.

76 FR 22448 - Delays in Processing of Special Permits Applications

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-21

..., AR. 8826-M Phoenix Air Group, Cartersville, 4 05-31-2011 GA. 10869-M Norris Cylinder Company, 4 05-31.... 10656-M Conference of Radiation Control 4 06-30-2011 Program Directors, Inc., Frankfort, KY. 11406-M Conference of Radiation Control 4 06-30-2011 Program Directors, Inc., Frankfort, KY. 14854-M Airgas, Inc...

76 FR 56493 - Notice of Delays in Processing of Special Permits Applications

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-13

.... 14741-M Weatherford 4 10-31-2011 International, Fort Worth, TX. 8826-M Phoenix Air 4 10-31-2011 Group... Seattle, WA. 10656-M Conference of 4 11-30-2011 Radiation Control Program Directors, Inc., Frankfort, KY. 11406-M Conference of 4 11-30-2011 Radiation Control Program Directors, Inc., Frankfort, KY. 12629-M TEA...

Alabama Public Scoping Meeting | NOAA Gulf Spill Restoration

Science.gov Websites

: Mobile, AL Start Time: 6:30 p.m. Central Time Description: As part of the public scoping process, the co open at 6:30 p.m. and the meeting will begin at 7:30 p.m. Location: The Battle House Renaissance Mobile Hotel & Spa 26 North Royal Street Mobile, AL 36602 (google map of location) Gulf Spill Restoration

16 CFR § 1505.5 - Electrical design and construction.

Code of Federal Regulations, 2013 CFR

2013-01-01

... lampholder contact which is at a potential of more than 30 volts r.m.s. (42.4 volts peak) to any other part... incandescent lamp having a voltage of more than 30 volts r.m.s. (42.4 volts peak) between any of its electrodes... any other part or ground is greater than 30 volts r.m.s. (42.4 volts peak), the contacts shall be...

16 CFR 1505.5 - Electrical design and construction.

Code of Federal Regulations, 2010 CFR

2010-01-01

... contact which is at a potential of more than 30 volts r.m.s. (42.4 volts peak) to any other part or to... having a voltage of more than 30 volts r.m.s. (42.4 volts peak) between any of its electrodes or... ground is greater than 30 volts r.m.s. (42.4 volts peak), the contacts shall be located in an insulating...

16 CFR 1505.5 - Electrical design and construction.

Code of Federal Regulations, 2011 CFR

2011-01-01

... contact which is at a potential of more than 30 volts r.m.s. (42.4 volts peak) to any other part or to... having a voltage of more than 30 volts r.m.s. (42.4 volts peak) between any of its electrodes or... ground is greater than 30 volts r.m.s. (42.4 volts peak), the contacts shall be located in an insulating...

16 CFR 1505.5 - Electrical design and construction.

Code of Federal Regulations, 2014 CFR

2014-01-01

... contact which is at a potential of more than 30 volts r.m.s. (42.4 volts peak) to any other part or to... having a voltage of more than 30 volts r.m.s. (42.4 volts peak) between any of its electrodes or... ground is greater than 30 volts r.m.s. (42.4 volts peak), the contacts shall be located in an insulating...

16 CFR 1505.5 - Electrical design and construction.

Code of Federal Regulations, 2012 CFR

2012-01-01

... contact which is at a potential of more than 30 volts r.m.s. (42.4 volts peak) to any other part or to... having a voltage of more than 30 volts r.m.s. (42.4 volts peak) between any of its electrodes or... ground is greater than 30 volts r.m.s. (42.4 volts peak), the contacts shall be located in an insulating...

76 FR 65750 - Advisory Board Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-24

... DEPARTMENT OF JUSTICE National Institute of Corrections Advisory Board Meeting Time and Date: 8 a.m. to 4:30 p.m. on Wednesday, November 2, 2011, 8 a.m. to 4:30 p.m. on Thursday, November 3, 2011. Place: Stanford University Law School, 550 Nathan Abbott Way, Stanford, California, (650) 724-6258...

Escherichia coli Ribosomal Protein S1 Unfolds Structured mRNAs Onto the Ribosome for Active Translation Initiation

PubMed Central

Duval, Mélodie; Korepanov, Alexey; Fuchsbauer, Olivier; Fechter, Pierre; Haller, Andrea; Fabbretti, Attilio; Choulier, Laurence; Micura, Ronald; Klaholz, Bruno P.; Romby, Pascale; Springer, Mathias; Marzi, Stefano

2013-01-01

Regulation of translation initiation is well appropriate to adapt cell growth in response to stress and environmental changes. Many bacterial mRNAs adopt structures in their 5′ untranslated regions that modulate the accessibility of the 30S ribosomal subunit. Structured mRNAs interact with the 30S in a two-step process where the docking of a folded mRNA precedes an accommodation step. Here, we used a combination of experimental approaches in vitro (kinetic of mRNA unfolding and binding experiments to analyze mRNA–protein or mRNA–ribosome complexes, toeprinting assays to follow the formation of ribosomal initiation complexes) and in vivo (genetic) to monitor the action of ribosomal protein S1 on the initiation of structured and regulated mRNAs. We demonstrate that r-protein S1 endows the 30S with an RNA chaperone activity that is essential for the docking and the unfolding of structured mRNAs, and for the correct positioning of the initiation codon inside the decoding channel. The first three OB-fold domains of S1 retain all its activities (mRNA and 30S binding, RNA melting activity) on the 30S subunit. S1 is not required for all mRNAs and acts differently on mRNAs according to the signals present at their 5′ ends. This work shows that S1 confers to the ribosome dynamic properties to initiate translation of a large set of mRNAs with diverse structural features. PMID:24339747

33 CFR 117.573 - Stoney Creek.

Code of Federal Regulations, 2013 CFR

2013-07-01

... opened only at 7:30 a.m. and 5 p.m. if any vessels are waiting to pass. (b) From 11 a.m. to 7 p.m. on Saturday and from 12 p.m. to 5 p.m. on Sunday, the draw need be opened only on the hour and half hour. (c... (S173) bridge, mile 0.9, in Riviera shall open on signal, except: (a) From 6:30 a.m. to 9 a.m. and from...

33 CFR 117.573 - Stoney Creek.

Code of Federal Regulations, 2011 CFR

2011-07-01

... opened only at 7:30 a.m. and 5 p.m. if any vessels are waiting to pass. (b) From 11 a.m. to 7 p.m. on Saturday and from 12 p.m. to 5 p.m. on Sunday, the draw need be opened only on the hour and half hour. (c... (S173) bridge, mile 0.9, in Riviera shall open on signal, except: (a) From 6:30 a.m. to 9 a.m. and from...

33 CFR 117.573 - Stoney Creek.

Code of Federal Regulations, 2010 CFR

2010-07-01

... opened only at 7:30 a.m. and 5 p.m. if any vessels are waiting to pass. (b) From 11 a.m. to 7 p.m. on Saturday and from 12 p.m. to 5 p.m. on Sunday, the draw need be opened only on the hour and half hour. (c... (S173) bridge, mile 0.9, in Riviera shall open on signal, except: (a) From 6:30 a.m. to 9 a.m. and from...

33 CFR 117.573 - Stoney Creek.

Code of Federal Regulations, 2012 CFR

2012-07-01

... opened only at 7:30 a.m. and 5 p.m. if any vessels are waiting to pass. (b) From 11 a.m. to 7 p.m. on Saturday and from 12 p.m. to 5 p.m. on Sunday, the draw need be opened only on the hour and half hour. (c... (S173) bridge, mile 0.9, in Riviera shall open on signal, except: (a) From 6:30 a.m. to 9 a.m. and from...

33 CFR 117.573 - Stoney Creek.

Code of Federal Regulations, 2014 CFR

2014-07-01

... opened only at 7:30 a.m. and 5 p.m. if any vessels are waiting to pass. (b) From 11 a.m. to 7 p.m. on Saturday and from 12 p.m. to 5 p.m. on Sunday, the draw need be opened only on the hour and half hour. (c... (S173) bridge, mile 0.9, in Riviera shall open on signal, except: (a) From 6:30 a.m. to 9 a.m. and from...

Validation of digital elevation models (DEMs) and comparison of geomorphic metrics on the southern Central Andean Plateau

NASA Astrophysics Data System (ADS)

Purinton, Benjamin; Bookhagen, Bodo

2017-04-01

In this study, we validate and compare elevation accuracy and geomorphic metrics of satellite-derived digital elevation models (DEMs) on the southern Central Andean Plateau. The plateau has an average elevation of 3.7 km and is characterized by diverse topography and relief, lack of vegetation, and clear skies that create ideal conditions for remote sensing. At 30 m resolution, SRTM-C, ASTER GDEM2, stacked ASTER L1A stereopair DEM, ALOS World 3D, and TanDEM-X have been analyzed. The higher-resolution datasets include 12 m TanDEM-X, 10 m single-CoSSC TerraSAR-X/TanDEM-X DEMs, and 5 m ALOS World 3D. These DEMs are state of the art for optical (ASTER and ALOS) and radar (SRTM-C and TanDEM-X) spaceborne sensors. We assessed vertical accuracy by comparing standard deviations of the DEM elevation versus 307 509 differential GPS measurements across 4000 m of elevation. For the 30 m DEMs, the ASTER datasets had the highest vertical standard deviation at > 6.5 m, whereas the SRTM-C, ALOS World 3D, and TanDEM-X were all < 3.5 m. Higher-resolution DEMs generally had lower uncertainty, with both the 12 m TanDEM-X and 5 m ALOS World 3D having < 2 m vertical standard deviation. Analysis of vertical uncertainty with respect to terrain elevation, slope, and aspect revealed the low uncertainty across these attributes for SRTM-C (30 m), TanDEM-X (12-30 m), and ALOS World 3D (5-30 m). Single-CoSSC TerraSAR-X/TanDEM-X 10 m DEMs and the 30 m ASTER GDEM2 displayed slight aspect biases, which were removed in their stacked counterparts (TanDEM-X and ASTER Stack). Based on low vertical standard deviations and visual inspection alongside optical satellite data, we selected the 30 m SRTM-C, 12-30 m TanDEM-X, 10 m single-CoSSC TerraSAR-X/TanDEM-X, and 5 m ALOS World 3D for geomorphic metric comparison in a 66 km2 catchment with a distinct river knickpoint. Consistent m/n values were found using chi plot channel profile analysis, regardless of DEM type and spatial resolution. Slope, curvature, and drainage area were calculated and plotting schemes were used to assess basin-wide differences in the hillslope-to-valley transition related to the knickpoint. While slope and hillslope length measurements vary little between datasets, curvature displays higher magnitude measurements with fining resolution. This is especially true for the optical 5 m ALOS World 3D DEM, which demonstrated high-frequency noise in 2-8 pixel steps through a Fourier frequency analysis. The improvements in accurate space-radar DEMs (e.g., TanDEM-X) for geomorphometry are promising, but airborne or terrestrial data are still necessary for meter-scale analysis.

Comparative study of three modified numerical spectrophotometric methods: An application on pharmaceutical ternary mixture of aspirin, atorvastatin and clopedogrel

NASA Astrophysics Data System (ADS)

Issa, Mahmoud Mohamed; Nejem, R.'afat Mahmoud; Shanab, Alaa Abu; Hegazy, Nahed Diab; Stefan-van Staden, Raluca-Ioana

2014-07-01

Three novel numerical methods were developed for the spectrophotometric multi-component analysis of capsules and synthetic mixtures of aspirin, atorvastatin and clopedogrel without any chemical separation. The subtraction method is based on the relationship between the difference in absorbance at four wavelengths and corresponding concentration of analyte. In this method, the linear determination ranges were 0.8-40 μg mL-1 aspirin, 0.8-30 μg mL-1 atorvastatin and 0.5-30 μg mL-1 clopedogrel. In the quotient method, 0.8-40 μg mL-1 aspirin, 0.8-30 μg mL-1 atorvastatin and 1.0-30 μg mL-1 clopedogrel were determine from spectral data at the wavelength pairs that show the same ratio of absorbance for other two species. Standard addition method was used for resolving ternary mixture of 1.0-40 μg mL-1 aspirin, 0.8-30 μg mL-1 atorvastatin and 2.0-30 μg mL-1 clopedogrel. The proposed methods were validated. The reproducibility and repeatability were found satisfactory which evidence was by low values of relative standard deviation (<2%). Recovery was found to be in the range (99.6-100.8%). By adopting these methods, the time taken for analysis was reduced as these methods involve very limited steps. The developed methods were applied for simultaneous analysis of aspirin, atorvastatin and clopedogrel in capsule dosage forms and results were in good concordance with alternative liquid chromatography.

Physiological and performance effects of carbohydrate gels consumed prior to the extra-time period of prolonged simulated soccer match-play.

PubMed

Harper, Liam D; Briggs, Marc A; McNamee, Ged; West, Daniel J; Kilduff, Liam P; Stevenson, Emma; Russell, Mark

2016-06-01

The physiological and performance effects of carbohydrate-electrolyte gels consumed before the 30min extra-time period of prolonged soccer-specific exercise were investigated. Randomised, double-blind, crossover. Eight English Premier League academy soccer players performed 120min of soccer-specific exercise on two occasions while consuming fluid-electrolyte beverages before exercise, at half-time and 90min. Carbohydrate-electrolyte (0.7±0.1gkg(-1) BM) or energy-free placebo gels were consumed ∼5min before extra-time. Blood samples were taken before exercise, at half-time and every 15min during exercise. Physical (15-m and 30-m sprint speed, 30-m sprint maintenance and countermovement jump height) and technical (soccer dribbling) performance was assessed throughout each trial. Carbohydrate-electrolyte gels improved dribbling precision (+29±20%) and raised blood glucose concentrations by 0.7±0.8mmoll(-1) during extra-time (both p<0.01). Supplementation did not affect sprint velocities (15m and 30m), 30-m sprint maintenance or dribbling speed as reductions compared to 0-15min values occurred at 105-120min irrespective of trial (all p<0.05). Plasma osmolality and blood sodium concentrations increased post-exercise vs. the opening 15min (p<0.05) but no effect of supplementation existed. Selected markers of physical performance (jump height, 30-m sprint velocity and 30-m repeated sprint maintenance) also reduced by >3% during half-time (all p<0.05). Carbohydrate-electrolyte gel ingestion raised blood glucose concentrations and improved dribbling performance during the extra-time period of simulated soccer match-play. Supplementation did not attenuate reductions in physical performance and hydration status that occurred during extra-time. Copyright © 2015 Sports Medicine Australia. Published by Elsevier Ltd. All rights reserved.

Medullary serotonergic neurones modulate the ventilatory response to hypercapnia, but not hypoxia in conscious rats.

PubMed

Taylor, Natalie C; Li, Aihua; Nattie, Eugene E

2005-07-15

Serotonergic neurones in the mammalian medullary raphe region (MRR) have been implicated in central chemoreception and the modulation of the ventilatory response to hypercapnia, and may also be involved in the ventilatory response to hypoxia. In this study, we ask whether ventilatory responses across arousal states are affected when the 5-hydroxytryptamine 1A receptor (5-HT1A) agonist (R)-(+)-8-hydroxy-2(di-n-propylamino)tetralin (DPAT) is microdialysed into the MRR of the unanaesthetized adult rat. Microdialysis of 1, 10 and 30 mM DPAT into the MRR significantly decreased absolute ventilation values(VE) during 7% CO2 breathing by 21%, 19% and 30%, respectively, in wakefulness compared to artificial cerebrospinal fluid (aCSF) microdialysis, due to decreases in tidal volume (VT) and not in frequency (f), similar to what occurred during non-rapid eye movement (NREM) sleep. The concentration-dependence of the hypercapnic ventilatory effect might be due to differences in tissue distribution of DPAT. DPAT (30 mM) changed room air breathing pattern by increasing f and decreasing VT. As evidenced by a sham control group, repeated experimentation and microdialysis of aCSF alone had no effect on the ventilatory response to 7% CO2 during wakefulness or sleep. Unlike during hypercapnia, microdialysis of 30 mM DPAT into the MRR did not change the ventilatory response to 10% O2. Additionally, 10 and 30 mM DPAT MRR microdialysis decreased body temperature, and 30 mM DPAT increased the percentage of experimental time in wakefulness. We conclude that serotonergic activity in the MRR plays a role in the ventilatory response to hypercapnia, but not to hypoxia, and that MRR 5-HT1A receptors are also involved in thermoregulation and arousal.

A randomized, double-blind, placebo-controlled pilot study of IV morphine-6-glucuronide for postoperative pain relief after knee replacement surgery.

PubMed

Romberg, Raymonda; van Dorp, Eveline; Hollander, Justus; Kruit, Michel; Binning, Alexander; Smith, Terry; Dahan, Albert

2007-01-01

To determine the dose-response effect of intravenous morphine-6-glucuronide (M6G) on acute postoperative pain. Patients undergoing knee replacement surgery under spinal anesthesia were randomly assigned to 1 of 4 single intravenous M6G doses, 0 (placebo), 10, 20, or 30 mg/70 kg, administered 150 minutes after the spinal anesthetic was given. Analgesic effects were evaluated by determining the cumulative patient controlled analgesia (PCA) morphine dose, consumed over a 12 and 24 hours period, after the initial dose of M6G. For pain assessments, a 10 cm visual analog scale was used. Data from 41 patients were evaluated (n=10, 10, 10, and 11 in the 0, 10, 20, and 30 mg M6G groups). Only at the highest M6G dose (30 mg/70 kg), morphine PCA consumption was significantly less compared with placebo: over the first 12 postoperative hours mean PCA morphine consumption was 3.0+/-2.0 mg/h after placebo and 1.4+/-0.5 mg/h after 30 mg M6G (P=0.03); over the first 24 h mean PCA morphine consumption was 2.5+/-2.1 mg after placebo and 1.0+/-0.4 mg after 30 mg M6G (P=0.04) (mean+/-SD). Visual analog scale values were similar across all groups during these time periods. The analgesic effect of M6G in postoperative pain was demonstrated with 30 mg/70 kg M6G superior to placebo. At this dose, M6G has a long duration of action as determined by a reduction in the use of morphine PCA over 12 and 24 hours.

The inhibitory effect of 3-amino-1,2,4-triazole on relaxation induced by hydroxylamine and sodium azide but not hydrogen peroxide or glyceryl trinitrate in rat aorta.

PubMed Central

Mian, K. B.; Martin, W.

1995-01-01

1. In this study we investigated the role of catalase in relaxation induced by hydroxylamine, sodium azide, glyceryl trinitrate and hydrogen peroxide in isolated rings of rat aorta. 2. Hydrogen peroxide (1 microM-1 mM)-induced concentration-dependent relaxation of phenylephrine (PE)-induced tone in endothelium-containing rings. In endothelium-denuded rings, however, higher concentrations (30 microM-1 mM) of hydrogen peroxide were required to produce relaxation. The endothelium-dependent component of hydrogen peroxide-induced relaxation was abolished following pretreatment with N(O)-nitro-L-arginine methyl ester (L-NAME, 30 microM). L-NAME (30 microM) had no effect, however, on hydrogen peroxide-induced relaxation in endothelium-denuded rings. 3. Pretreatment of endothelium-denuded rings with catalase (1000 u ml-1) blocked relaxation induced by hydrogen peroxide (10 microM-1 mM). The ability of catalase to inhibit hydrogen peroxide-induced relaxation was partially blocked following incubation with 3-amino-1,2, 4-triazole (AT, 50 mM) for 30 min and completely blocked at 90 min. 4. Pretreatment of endothelium-denuded rings with methylene blue (MeB, 30 microM) inhibited relaxation induced by hydrogen peroxide (10 microM-1 mM), sodium azide (1-300 nM), hydroxylamine (1-300 nM) and glyceryl trinitrate (1-100 nM) suggesting that each acted by stimulation of soluble guanylate cyclase. 5. Pretreatment of endothelium-denuded rings with AT (1-50 mM, 90 min) to inhibit endogenous catalase blocked relaxation induced by sodium azide (1-300 nM) and hydroxylamine (1-300 nM) but had no effect on relaxation induced by hydrogen peroxide (10 microM-1 mM) or glyceryl trinitrate (1-100 nM). 6. In a cell-free system, incubation of sodium azide (10 microM-3 mM) and hydroxylamine (10 microM-30 mM) but not glyceryl trinitrate (10 microM-1 mM) with catalase (1000 u ml-1) in the presence of hydrogen peroxide (1 mM) led to production of nitrite, a major breakdown product of nitric oxide. AT (1-100 mM) inhibited, in a concentration-dependent manner, the formation of nitrite from azide in the presence of hydrogen peroxide. 7. These data suggest that metabolism by catalase plays an important role in the relaxation induced by hydroxylamine and sodium azide in isolated rings of rat aorta. Relaxation appears to be due to formation of nitric oxide and activation of soluble guanylate cyclase. In contrast, metabolism by catalase does not appear to be involved in the relaxant actions of hydrogen peroxide or glyceryl trinitrate. PMID:8719811

New Standards for Diagnosing Hypertension Are Met with Skepticism | Poster

Cancer.gov

Check your blood pressure for free at Occupational Health Services every Tuesday and Thursday as part of Blood Pressure Awareness Month. Free screening is available every Tuesday during the Farmers’ Market in Building 549 from 11:30 a.m. to 1:30 p.m, and every Tuesday and Thursday in Building 426 from 12:30 to 1 p.m. Sarah Hooper, manager of Occupational Health Services (OHS),

The effects of verapamil and diltiazem on N-, P- and Q-type calcium channels mediating dopamine release in rat striatum

PubMed Central

Dobrev, Dobromir; Milde, Alexander S; Andreas, Klaus; Ravens, Ursula

1999-01-01

The putative inhibitory effects of verapamil and diltiazem on neuronal non-L-type Ca2+ channels were studied by investigating their effects on either K+- or veratridine-evoked [3H]-dopamine ([3H]-DA) release in rat striatal slices. Involvement of N-, P- and Q-type channels was identified by sensitivity of [3H]-DA release to ω-conotoxin GVIA (ω-CTx-GVIA), ω-agatoxin IVA (ω-Aga-IVA) and ω-conotoxin MVIIC (ω-CTx-MVIIC), respectively.KCl (50 mM)-evoked [3H]-DA release was abolished in the absence of Ca2+, and was insensitive to dihydropyridines (up to 30 μM). It was significantly blocked by ω-CTx-GVIA (1 μM), ω-Aga-IVA (30 nM) and was confirmed to be abolished by ω-CTx-MVIIC (3 μM), indicating involvement of N-, P- and Q-type channel subtypes.Verapamil and diltiazem inhibited K+-evoked [3H]-DA release in a concentration-dependent manner. The inhibitory effects of verapamil or diltiazem (each 30 μM) were fully additive to the effect of ω-CTx-GVIA (1 μM), whereas co-application with ω-Aga-IVA (30 nM) produced similar effects to those of ω-Aga-IVA alone.As shown previously, veratridine-evoked [3H]-DA release in Ca2+ containing medium exclusively involves Q-type Ca2+ channels. Here, diltiazem (30 μM) did not inhibit veratridine-evoked [3H]-DA release, whereas verapamil (30 μM) partially inhibited it, indicating possible involvement of Q-type channels in verapamil-induced inhibition. However, verapamil (30 μM) inhibited this release even in the absence of extracellular Ca2+, suggesting that Na+ rather than Q-type Ca2+ channels are involved.Taken together, our results suggest that verapamil can block P- and at higher concentrations possibly N- and Q-type Ca2+ channels linked to [3H]-DA release, whereas diltiazem appears to block P-type Ca2+ channels only. PMID:10385261

77 FR 52023 - Meeting of the National Drinking Water Advisory Council

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-28

... from 8:30 a.m. to 5:00 p.m., Central Time, and on October 5, 2012, from 8:30 a.m. to 3:00 p.m., Central Time. ADDRESSES: The meeting will be held at EPA's Chicago Regional Office (EPA Region 5) at the Ralph... the public's input (1:00 p.m.-2:00 p.m., Central Time) at the meeting on October 5, 2012. Oral...

75 FR 56516 - Federal Advisory Committee; Military Leadership Diversity Commission (MLDC)

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-16

... Steven A. Hady, Designated Federal Officer, MLDC, at (703) 602-0838 or (571) 882-0140, 1851 South Bell... September 27 (from 7 p.m. to 9:30 p.m.), September 28 (from 8 a.m. to 8 p.m.), and September 29, 2010 (from 8 a.m. to 5:30 p.m.). An Administrative Working Meeting that is scheduled for September 27 from 7 to...

Site-activated chelators derived from anti-Parkinson drug rasagiline as a potential safer and more effective approach to the treatment of Alzheimer's disease.

PubMed

Zheng, Hailin; Fridkin, Mati; Youdim, Moussa B H

2010-12-01

chelators can modulate β-amyloid accumulation, protect against tau hyperphosphorylation, and block metal-related oxidative stress, and thereby hold considerable promise as effective anti-AD drugs. At present, a growing interest is focusing on increasing the efficacy and targeting of chelators through drug design. To this end, we have developed a new class of multifunctional prochelators from three FDA- approved drugs rasagiline, rivastigmine, and donepezil or tacrine. HLA20 A was designed by merging the important pharmacophores of rasagiline, rivastigmine, and donepezil into our newly developed multifunctional chelator HLA20. M30D was constructed using the key pharmacophoric moieties from rasagiline, rivastigmine, and tacrine. Experiments showed that both compounds possess potent anti-acetylcholinesterase (AChE) activity in vitro with weak inhibition of butyrylcholinesterase (BuChE), and without significant metal-binding activity. M30D was found also to be a highly potent MAO A inhibitor with moderate inhibition of MAO B in vitro. Both HLA20 and M30D can be activated by inhibition of AChE to release active chelators HLA20 and M30, respectively. HLA20 and M30 have been shown to be able to modulate amyloid precursor protein regulation and beta-amyloid reduction, suppress oxidative stress, and passivate excess metal ions (Fe, Cu, and Zn). Compared with the activated chelator HLA20 or M30, both HLA20A and M30D exhibited lower cytotoxicity in SH-SY5Y neuroblastoma cells, substantiating the prochelator strategy for minimizing toxicity associated with poor targeted chelators.

Effect of individual SCFA on the epithelial barrier of sheep rumen under physiological and acidotic luminal pH conditions.

PubMed

Greco, Gabriele; Hagen, Franziska; Meißner, Svenja; Shen, Zanming; Lu, Zhongyan; Amasheh, Salah; Aschenbach, Jörg R

2018-02-15

The objective of this study was to investigate whether individual short-chain fatty acids (SCFA) have a different potential to either regulate the formation of the ruminal epithelial barrier (REB) at physiological pH or to damage the REB at acidotic ruminal pH. Ruminal epithelia of sheep were incubated in Ussing chambers on their mucosal side in buffered solutions (pH 6.1 or 5.1) containing no SCFA (control), 30 mM of either acetate, propionate or butyrate, or 100 mM acetate. Epithelial conductance (Gt), short-circuit current (Isc), and fluorescein flux rates were measured over 7 h. Thereafter, mRNA and protein abundance, as well as localization of the tight junction proteins claudin (Cldn)-1, -4, -7, and occludin were analyzed. At pH 6.1, butyrate increased Gt and decreased Isc, with additional decreases in claudin-7 mRNA and protein abundance (each P < 0.05) and disappearance of Cldn-7 immunosignals from the stratum corneum. By contrast, the mRNA abundance of Cldn-1 and/or Cldn-4 were upregulated by 30 mM propionate, 30 mM butyrate, or 100 mM acetate (P < 0.05), however, without coordinated changes in protein abundance. At luminal pH 5.1, neither Gt, Isc, nor TJ protein abundance was altered in the absence of SCFA; only fluorescein flux rates were slightly increased (P < 0.05) and fluorescein signals were no longer restricted to the stratum corneum. The presence of acetate, propionate, or butyrate at pH 5.1 increased fluorescein flux rates and Gt, and decreased Isc (each P < 0.05). Protein abundance of Cldn-1 was decreased in all SCFA treatments but 30 mM butyrate; abundance of Cldn -4 and -7 was decreased in all SCFA treatments but 30 mM acetate; and abundance of occludin was decreased in all SCFA treatments but 30 mM propionate (each P < 0.05). Immunofluorescence staining of SCFA-treated tissues at pH 5.1 showed disappearance of Cldn-7, discontinuous pattern for Cldn-4 and blurring of occludin and Cldn-1 signals in tight junction complexes. The fluorescein dye appeared to freely diffuse into deeper cell layers. The strongest increase in Gt and consistent decreases in the abundance and immunosignals of tight junction proteins were observed with 100 mM acetate at pH 5.1. We conclude that SCFA may contribute differently to the REB formation at luminal pH 6.1 with possible detrimental effects of butyrate at 30 mM concentration. At luminal pH 5.1, all SCFA elicited REB damage with concentration appearing more critical than SCFA species.

75 FR 6729 - Meeting of National Council on the Humanities

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-10

... Applications and Programs Before the Council 10:30 a.m. until Adjourned Digital Humanities--Room M-07 Education...: Committee Meetings (Open to the Public) Policy Discussion 9-10:30 a.m. Digital Humanities--Room M-07 Education Programs and Federal/State Partnership--Room 510A Preservation and Access--Room 415 Public...

76 FR 20051 - Advisory Committee for International Science & Engineering; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-11

... NATIONAL SCIENCE FOUNDATION Advisory Committee for International Science & Engineering; Notice of... Engineering ( 25104). Date/Time: April 25, 2011; 8:30 a.m. to 5 p.m. April 26, 2011; 8:30 a.m. to 12 p.m... International Science and Engineering, Reports from Advisory Committee Working Groups. April 26, 2011 AM...

77 FR 48552 - Meetings of Humanities Panel

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-14

... room numbers. FOR FURTHER INFORMATION CONTACT: Lisette Voyatzis, Committee Management Officer, 1100...: September 6, 2012. Time: 8:30 a.m. to 5:00 p.m. Room: 415. This meeting will discuss applications for the... Access. 2. Date: September 6, 2012. Time: 8:30 a.m. to 5:00 p.m. Room: 421. This meeting will discuss...

77 FR 778 - Council Coordination Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-06

... meeting of the Council Coordination Committee (CCC), consisting of the Regional Fishery Management Council... this meeting and provide reports to the CCC for its information and discussion. All sessions are open...:45 p.m.--Other Business, updates, and next annual CCC Meeting. 4:45 p.m.-5:30 p.m.--Wrap-up. 5:30 p.m...

Development of Bottom Oil Recovery Systems. Revised

DTIC Science & Technology

2014-02-01

designed a recovery system based on dredging technology. It could handle harsh wind /wave conditions but has significant logistical requirements, due...Knots m/s Meter(s) per second M/T Motor tanker M/V Motor vessel m Meter or meters m2 Square meters m3 Cubic meters MBTA Migratory Bird ...usable for some bottom types. Wind 30 kts (45-kt gusts) Wave 0-2m (0-5ft) Current 0-2 kts Lightning ɝmiles Minimum depth of about 9m (30 ft

Role of 11β-OH-C(19) and C(21) steroids in the coupling of 11β-HSD1 and 17β-HSD3 in regulation of testosterone biosynthesis in rat Leydig cells.

PubMed

Latif, Syed A; Shen, Mae; Ge, Ren-Shan; Sottas, Chantal M; Hardy, Matthew P; Morris, David J

2011-06-01

Here we describe further experiments to support our hypothesis that bidirectional 11β-HSD1-dehydrogenase in Leydig cells is a NADP(H) regenerating system. In the absence of androstenedione (AD), substrate for 17β-HSD3, incubation of Leydig cells with corticosterone (B) or several C(19)- and C(21)-11β-OH-steroids, in the presence of [(3)H]-11-dehydro-corticosterone (A), stimulated 11β-HSD1-reductase activity. However, in presence of 30 μM AD, testosterone (Teso) synthesis is stimulated from 4 to 197 picomole/25,000 cells/30 min and concomitantly inhibited 11β-HSD1-reductase activity, due to competition for the common cofactor NADPH needed for both reactions. Testo production was further significantly increased (p<0.05) to 224-267 picomole/25,000 cells/30 min when 10 μM 11β-OH-steroids (in addition to 30 μM AD) were also included. Similar results were obtained in experiments conducted with lower concentrations of AD (5 μM), and B or A (500 nM). Incubations of 0.3-6.0 μM of corticosterone (plus or minus 30 μM AD) were then performed to test the effectiveness of 17β-HSD3 as a possible NADP(+) regenerating system. In the absence of AD, increasing amounts (3-44 pmol/25,000 cells/30 min) of 11-dehydro-corticosterone were produced with increasing concentrations of corticosterone in the medium. When 30 μM AD was included, the rate of 11-dehydro-corticosterone formation dramatically increased 1.3-5-fold producing 4-210 pmol/25,000 cells/30 min of 11-dehydro-corticosterone. We conclude that 11β-HSD1 is enzymatically coupled to 17β-HSD3, utilizing NADPH and NADP in intermeshed regeneration systems. Copyright © 2011 Elsevier Inc. All rights reserved.

75 FR 62551 - Center for Scientific Review; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-12

... Conflict: Biobehavioral Mechanisms of Emotion, Stress and Health. Date: November 3, 2010. Time: 2 p.m. to 5...-08-222: International Brain Disorders. Date: November 18, 2010. Time: 8:30 a.m. to 6:30 p.m. Agenda...

78 FR 38410 - Arts Advisory Panel Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-26

... (application review): Room 730. This meeting will be closed. Dates: July 15, 2013; 9:00 a.m. to 5:30 p.m. Music...:30 p.m. Music (application review): Room 714. This meeting will be closed. Dates: July 16, 2013; 10...

Manufacturing Methods and Technology. Project Summary Reports.

DTIC Science & Technology

1980-06-01

Tung- sten Inert Gas (TIG) welding of aluminum components. 41 X 4 (,B)IRAIt AW k A r A’I 4 " 6 A T A k I, A ., : , . Figure 1 - Modular Packages for...Telescope and M134 Mount 75 6 M102 Howitzer M113 Panoramic Telescope and M134 Mount 100 4 )21l4 Quiadrant 1.50 2 XM11A Elbow Telescope 130 5 M29...No. Temperature( OF) Time at temperature(min.) 1 200 30 2 200 6o 3 300 30 4 300 60 5 4DO 30 6 4 +0 60 Springs compressed to a stress level of 150,000

In-Vitro Analysis of Glucose and Quercetin Effects on m-TOR and Nrf-2 Expression in HepG2 Cell Line (Diabetes and Cancer Connection).

PubMed

Yarahmadi, Amir; Khademi, Fatemeh; Mostafavi-Pour, Zohreh; Zal, Fatemeh

2018-05-21

Some types of cancers show a strong relationship with diabetes and play a central role in mortality in the patient population suffering from diabetes mellitus. In this study, HepG2 cells have been used to investigate the toxic effects of hyperglycemia and/or quercetin (Q) on mammalian target of rapamycin (m-TOR) and nuclear factor erythroid 2-related factor 2 (Nrf-2) expression as central molecules involved in cancer. HepG2 cells were cultured with different concentrations of glucose (5.5, 30, and 50 mM) and/or Q (25 µM) for 48 and 72 h. Effects of glucose and/or Q on m-TOR and Nrf-2 expression were assayed by quantitative real-time PCR (qRT-PCR). qRT-PCR results revealed that 30 and 50 mM of glucose increased m-TOR expression at 48 h, although after 72 h, only 30 mM had an increasing effect. At 50 mM, glucose-induced Nrf-2 gene expression after both 48 and 72 h. The results also showed that 25 µM of Q reduced m-TOR and Nrf-2 expression at both 30 and 50 mM after 48 and 72 h incubation. Q has potential effects on reducing oxidative stress caused by hyperglycemia and during diabetes may be able to modulate some carcinogenic signaling pathways.

77 FR 32021 - Safety Zones: Fireworks Displays in the Captain of the Port Columbia River Zone

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-31

... follows: (1) Portland Rose Festival Fireworks Display, Portland, OR: May 25, 2012 from 8:30 p.m. until 11... p.m. until 11:30 p.m. (5) Oregon Food Bank Blues Festival Fireworks, Portland, OR: July 4, 2012 from.... (12) Splash Aberdeen Waterfront Festival, Aberdeen, WA: July 4, 2012 from 9 p.m. until 11 p.m. (13...

Relationship of the stretch-shortening cycle to sprint performance in trained female athletes.

PubMed

Hennessy, L; Kilty, J

2001-08-01

This study assessed the relationship of long and short stretch-shortening cycle test scores to sprint performances in trained female athletes. Seventeen trained, female, high school, competitive sprinters completed the following tests: countermovement jump for vertical distance (CMJ), bounce drop jump for height with minimum ground contact time (BDJ index), and ground contact time (GCT) during the BDJ and a 5-step bound (5B) test. Group mean and SD values were as follows: height, 167.7 +/- 3.7 cm; body mass, 59.9 +/- 7.2 kg; and percentage of body fat (PF), 20.3 +/- 1.8%. Sprint performances at 30-, 100-, and 300-m distances were assessed. Stretch-shortening cycle performance and sprint results (mean +/- SD) were as follows: CMJ, 33.8 +/- 3.8 cm; BDJ index, 166.7 +/- 24.7 cm/s; 5B test, 10.98 +/- 0.76 m; 30-m sprint, 4.58 +/- 0.17 seconds; 100-m sprint, 12.9 +/- 0.61 seconds; and 300-m sprint, 45.03 +/- 2.94 seconds. Correlations indicated that no relationship existed between PF and the dependent sprint variables. Significant correlations (p < 0.05) existed between CMJ and 30-m (r = -0.60), 100-m (r = -0.64), and 300-m (r = -0.55) sprint times; BDJ index and 30-m (r = -0.79) and 100-m (r = -0.75) sprint times; and 5B test and 300-m sprint time (r = -0.54). Multiple regression analysis found significant T values for BDJ index with 30- and 100-m sprints and CMJ and PF with 300 m. Results indicated that the BDJ index and CMJ tests were significantly related to sprint performances in female athletes.

76 FR 21780 - Notice of Public Meeting; Central Montana Resource Advisory Council

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-18

... during normal business hours. The FIRS is available 24 hours a day, 7 days a week to leave a message or... meeting will be held May 3 and 4, 2011. The May 3 meeting will begin at 10 a.m. with a 30-minute public comment period and will adjourn at 5:30 p.m. The May 4 meeting will begin at 8 a.m. with a 30- minute...

78 FR 55266 - National Institute of Diabetes and Digestive and Kidney Diseases; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-10

... Diseases and Nutrition. Date: October 22, 2013. Time: 8:00 a.m. to 4:00 p.m. Agenda: To review and evaluate... in Diabetic Ketoacidosis (U34) Date: October 21, 2013. Time: 9:30 a.m. to 11:30 a.m. Agenda: To... Panel; DDK-C Conflicts Date: October 22, 2013. Time: 4:00 p.m. to 6:00 p.m. Agenda: To review and...

Selected Mildly Obese Donors Can Be Used Safely in Simultaneous Pancreas and Kidney Transplantation.

PubMed

Alhamad, Tarek; Malone, Andrew F; Lentine, Krista L; Brennan, Daniel C; Wellen, Jason; Chang, Su-Hsin; Chakkera, Harini A

2017-06-01

Donor obesity, defined as donor body mass index (D-BMI) of 30 kg/m or greater, has been associated with increased risk of technical failure and poor pancreas allograft outcomes. Many transplant centers establish a threshold of D-BMI of 30 kg/m to decline donor offers for pancreas transplantation. However, no previous studies differentiate the impact of mild (D-BMI, 30-35 kg/m) versus severe obesity (D-BMI, ≥35 kg/m) on pancreas allograft outcomes. We examined Organ Procurement Transplant Network database records for 9916 simultaneous pancreas-kidney transplants (SPKT) performed between 2000 and 2013. We categorized donor body mass index (D-BMI) into 4 groups: 20 to 25 (n = 5724), 25 to 30 (n = 3303), 30 to 35 (n = 751), and 35 to 50 kg/m (n= 138). Associations of D-BMI with pancreas and kidney allograft failure were assessed by multivariate Cox regression adjusted for recipient, donor, and transplant factors. Compared with D-BMI 20 to 25 kg/m, only D-BMI 35 to 50 kg/m was associated with significantly higher pancreas allograft [adjusted hazard ratio [aHR], 1.37; 95% confidence interval (CI], 1.04-1.79] and kidney allograft (aHR, 1.36; CI, 1.02-1.82) failure over the study period (13 years). Donor BMI 30 to 35 kg/m did not impact pancreas allograft (aHR, 0.99; CI, 0.86-1.37) or kidney allograft (aHR, 0.98; CI, 0.84-1.15) failure. Similar patterns were noted at 3 months, and 1, 5, and 10 years posttransplant. These data support that pancreata from mildly obese donors (BMI, 30-35 kg/m) can be safely used for transplantation, with comparable short-term and long-term outcomes as organs from lean donors. Consideration of pancreata from obese donors may decrease the pancreas discard rate.

Shifting reef fish assemblages along a depth gradient in Pohnpei, Micronesia

PubMed Central

Copus, Joshua M.; Coffey, Daniel M.; Whitton, Robert K.; Bowen, Brian W.

2018-01-01

Mesophotic coral ecosystems (MCEs) continue to be understudied, especially in island locations spread across the Indo-Pacific Ocean. Pohnpei is the largest island in the Federated States of Micronesia, with a well-developed barrier reef, and steep slopes that descend to more than 1,000 m. Here we conducted visual surveys along a depth gradient of 0 to 60 m in addition to video surveys that extend to 130 m, with 72 belt transects and 12 roving surveys using closed-circuit rebreathers, to test for changes in reef fish composition from shallow to mesophotic depths. We observed 304 fish species across 47 families with the majority confined to shallow habitat. Taxonomic and trophic positions at 30 m showed similar compositions when compared against all other depths. However, assemblages were comprised of a distinct shallow (<30 m) and deep (>30 m) group, suggesting 30 m as a transition zone between these communities. Shallow specialists had a high probability of being herbivores and deep specialists had a higher probability of being planktivores. Acanthuridae (surgeonfishes), Holocentridae (soldierfishes), and Labridae (wrasses) were associated primarily with shallow habitat, while Pomacentridae (damselfishes) and Serranidae (groupers) were associated with deep habitat. Four species may indicate Central Pacific mesophotic habitat: Chromis circumaurea, Luzonichthys seaver, Odontanthias borbonius, and an undescribed slopefish (Symphysanodon sp.). This study supports the 30 m depth profile as a transition zone between shallow and mesophotic ecosystems (consistent with accepted definitions of MCEs), with evidence of multiple transition zones below 30 m. Disturbances restricted to either region are not likely to immediately impact the other and both ecosystems should be considered separately in management of reefs near human population centers. PMID:29707432

Plasma levels of soluble CD30 in kidney graft recipients as predictors of acute allograft rejection.

PubMed

Ayed, K; Abdallah, T B; Bardi, R; Abderrahim, E; Kheder, A

2006-09-01

In renal transplant recipients elevated soluble serum CD30 levels are associated with increased rejection and graft loss. We sought to determine the sCD30 plasma levels before and after kidney transplantation and to assess whether sCD30 was a predictive factor of immunological risk. sCD30 plasma levels were determined by an enzyme-linked immunosorbent assay assay in 52 kidney graft recipients before as well as 7, 15, and 21 days after transplantation. Eighteen patients developed acute allograft rejection (group I) and 34 patients showed uneventful courses (group II). Before transplantation sCD30 plasma levels were elevated in both groups (mean: 162.6 +/- 89.5 U/mL). After transplantation, group I recipients with acute rejection showed higher relative levels of plasma sCD30 on days 7 and 15 (120.8 +/- 74.6 U/mL and 210.6 +/- 108.7 U/mL respectively) compared with group II patients without rejection (95 +/- 45 U/mL and 59.4 +/- 31.6 U/mL), a difference that was significant for group I (P = .0003) and not significant for group II (P = .09). On day 21, sCD30 decreased in the two groups but remained higher among group I patients (120.6 +/- 92.7 U/mL). HLA antibodies were positive in 18 patients (34.6%) with 9 (50%) experiencing at last one episode of acute rejection. Among 34 patients negative for anti-HLA antibodies, nine displayed acute rejection only (26.4%), a difference that was not significant (P > .05). If we consider 100 U/mL as the minimum predictive level for allograft rejection, our results suggested that levels of sCD30 should be taken into consideration with the presence of HLA-antibodies detectable before and after transplantation, especially in patients with more than three HLA mismatches [RR = 3.20 (0.94 < RR < 10.91)]. These data suggested that measurement of plasma sCD30 is a useful procedure for the recognition of rejection in its earliest stages.

75 FR 40726 - Safety Zones: Annual Events Requiring Safety Zones in the Captain of the Port Buffalo Zone

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-14

... July 5, 2010 from 10 p.m. to 10:30 p.m. 12. Mentor Harbor Yacht Club Fireworks Celebration on Lake Erie, Mentor Harbor, OH in 33 CFR 165.939(a)(19) on July 3, 2010 from 10 p.m. to 10:30 p.m. 13. City of...

78 FR 49550 - Meetings of Humanities Panel

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2013-08-14

... Division of Education Programs. 2. Date: September 09, 2013. Time: 8:30 a.m. to 5:00 p.m. Room: 421. This... Initiatives at Tribal Colleges and Universities grant program, submitted to the Division of Education Programs... Division of Education Programs. 6. Date: September 12, 2013. Time: 8:30 a.m. to 5:00 p.m. Room: 421. This...

77 FR 76058 - National Heart, Lung, and Blood Institute; Notice of Closed Meetings

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2012-12-26

.... Time: 2:00 p.m. to 3:00 p.m. Agenda: To review and evaluate contract proposals. Place: Crystal City.... Date: January 15, 2013. Time: 8:30 a.m. to 11:30 a.m. Agenda: To review and evaluate contract proposals. Place: Crystal City Marriott, 1999, Jefferson Davis Highway, Arlington, VA 22202. Contact Person...

77 FR 27115 - Newport to Bermuda Regatta, Narragansett Bay, Newport, RI

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-09

... Bermuda Regatta, Narragansett Bay, Rhode Island, from 10 a.m. to 3:30 p.m. on Friday, June 15, 2012... will be enforced from 10 a.m. to 3:30 p.m. on June 15, 2012. FOR FURTHER INFORMATION CONTACT: Mr. Edward LeBlanc, Chief, Waterways Management Division, Sector Southeastern New England, (401) 435-2351...

76 FR 24921 - Proposal Review Panel for Chemistry; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-03

... NATIONAL SCIENCE FOUNDATION Proposal Review Panel for Chemistry; Notice of Meeting In accordance... announces the following meeting: Name: Proposal Review Panel for Chemistry, 1191. Date and Time: May 12, 2011, 8:30 a.m.-5 p.m.; May 13, 2011, 8:30 a.m.-2 p.m. Place: Center for Chemistry at the Space-Time...

17 CFR 240.0-2 - Business hours of the Commission.

Code of Federal Regulations, 2014 CFR

2014-04-01

..., except Saturdays, Sundays, and Federal holidays, from 9 a.m. to 5:30 p.m., Eastern Standard Time or... Saturdays, Sundays and federal holidays, from 8 a.m. to 5:30 p.m., Eastern Standard Time or Eastern Daylight... the filing of documents pursuant to the Act or the rules and regulations thereunder are as set forth...

17 CFR 240.0-2 - Business hours of the Commission.

Code of Federal Regulations, 2013 CFR

2013-04-01

..., except Saturdays, Sundays, and Federal holidays, from 9 a.m. to 5:30 p.m., Eastern Standard Time or... Saturdays, Sundays and federal holidays, from 8 a.m. to 5:30 p.m., Eastern Standard Time or Eastern Daylight... the filing of documents pursuant to the Act or the rules and regulations thereunder are as set forth...

17 CFR 232.12 - Business hours of the Commission.

Code of Federal Regulations, 2014 CFR

2014-04-01

..., is open each day, except Saturdays, Sundays, and federal holidays, from 9 a.m. to 5:30 p.m., Eastern... federal holidays, from 8 a.m. to 5:30 p.m., Eastern Standard Time or Eastern Daylight Saving Time... the filing of documents pursuant to the Acts or the rules and regulations thereunder are as set forth...

17 CFR 232.12 - Business hours of the Commission.

Code of Federal Regulations, 2013 CFR

2013-04-01

..., is open each day, except Saturdays, Sundays, and federal holidays, from 9 a.m. to 5:30 p.m., Eastern... federal holidays, from 8 a.m. to 5:30 p.m., Eastern Standard Time or Eastern Daylight Saving Time... the filing of documents pursuant to the Acts or the rules and regulations thereunder are as set forth...

75 FR 9001 - Advisory Committee for Environmental Research and Education; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-26

.... March 19, 2010 Discussion on Expanding NSF Research Portfolio for Science, Engineering and Education for... NATIONAL SCIENCE FOUNDATION Advisory Committee for Environmental Research and Education; Notice of... Research and Education (9487). Dates: March 18, 2010, 8:30 a.m.-5 p.m. March 19, 2010, 8:30 a.m.-1 p.m...

76 FR 7881 - Advisory Committee for Environmental Research and Education; Notice of Meeting

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2011-02-11

.... March 17 Update on NSF's Science, Engineering and Education for Sustainability portfolio (SEES). Dated... NATIONAL SCIENCE FOUNDATION Advisory Committee for Environmental Research and Education; Notice of... and Education, 9487. Dates: March 16, 2011, 8:30 a.m.-5 p.m. and March 17, 2011, 8:30 a.m.-2 p.m...

77 FR 6826 - Advisory Committee for Environmental Research and Education; Notice of Meeting

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2012-02-09

... Thursday, March 15, 2012 Update on NSF's Science, Engineering and Education for Sustainability portfolio... NATIONAL SCIENCE FOUNDATION Advisory Committee for Environmental Research and Education; Notice of... and Education (9487). Dates: March 14, 2012, 8:30 a.m.- 5 p.m.; March 15, 2012, 8:30 a.m.-2 p.m. Place...

75 FR 2114 - Military Leadership Diversity Commission (MLDC); Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-14

... Administrative Working Meeting; 8 a.m. to 5:30 p.m., February 11, 2010; 8 a.m. to 5:30 p.m., February 12, 2010..., Hampton, VA 23669. FOR FURTHER INFORMATION CONTACT: Master Chief Steven A. Hady, Designated Federal Officer, MLDC, at (703) 602-0838 or (571) 882-0140, 1851 South Bell Street, Suite 532, Arlington, Va. E...

75 FR 37307 - Drawbridge Operation Regulations; Milwaukee, Menomonee, and Kinnickinnic Rivers and South...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-29

... effective from 9:30 p.m. to 1 a.m. on June 24, 2010 and July 3, 2010. A rain date of June 25 and July 4... including the rain date of June 25, 2010 the bridges need not open for any vessel from 9:30 p.m. to 1 a.m...

Low Frequency Seismo-Acoustic Propagation in a Sloping Ocean Environment: Measured Results and Numerical Predictions

DTIC Science & Technology

1987-11-01

15407 - 1525,1 m/s Cp = 1900 m/ s , op = 0,2, p= 1.8 L . Cp = 2340 m/ s . Op = 02, p= 1,9 Cp = 3000 m/ s . Op = 02, P = 2,2 Cj = 1500 m/s, oj = 02 Cp...4300 m/ s , op = 005, p = 24 Cc = 2150 m/s, o^ = 01 20 Figure 5. Geoacoustic input parameters for SAFARI model. 0.0 10.0 30.0 30.0 RANGE IKM

Ionic mechanisms of transient inward current in the absence of Na(+)-Ca2+ exchange in rabbit cardiac Purkinje fibres.

PubMed Central

Han, X; Ferrier, G R

1992-01-01

1. Membrane currents were measured with a two-microelectrode technique in voltage clamped rabbit cardiac Purkinje fibres under conditions known to cause intracellular calcium overload and to eliminate or minimize Na(+)-Ca2+ exchange. 2. Increasing [Ca2+]o from 2.5 to 5 mM or above and substituting external sodium with either sucrose, choline or Li+ induced an oscillatory transient inward current (TI) which peaked 200-300 ms after repolarization from a previous depolarizing pulse. The TI quickly disappeared upon return to normal Tyrode solution. Both the rate and configuration of action potentials of Purkinje fibres also returned to control upon return to Tyrode solution after 30 min of high Ca2+ exposure, if the Ca2+ concentration was 30 mM or less. 3. The TI in Na(+)-free solution was Ca2+ dependent. Either zero or low (2.5 mM) [Ca2+]o, or replacement of [Ca2+]o by BaCl prevented induction of the TI current upon repolarization from a previous depolarizing pulse. 4. In the presence of 30 mM-CaCl2 and with choline chloride as the substitute for NaCl, TI had a distinct reversal potential (Erev) of -25 mV. The time-to-peak TI, either inward or outward, did not shift significantly with change in voltage. Both inward and outward TI were simultaneously abolished by exposure to 1 microM-ryanodine, suggesting they were both activated by transient release of Ca2+ from the sarcoplasmic reticulum. The occurrence of TI in the absence of [Na+]o is not compatible with an electrogenic Na(+)-Ca2+ exchange mechanism. The existence of a clear-cut reversal potential suggests that an ionic channel may be responsible for the TI under these conditions. 5. Both the magnitude of peak TI and the Erev were affected by changes of CaCl2 concentration. (i) Under steady-state conditions, peak inward TI was significantly increased when the [Ca2+]o was elevated from 5 to 15 mM. The peak TI in the outward direction was significantly increased when [Ca2+]o was elevated from 15 to 30 mM; however, the difference in peak inward TI at 15 and 30 mM [Ca2+]o was small. (ii) Clear-cut reversals of TI were found at Ca2+ concentrations of 10 mM (Erev = -19.5 mV) or greater, and elevation of [Ca2+]o to 20, 30, 50 and 105 mM shifted the Erev to more negative potentials. (iii) In the presence of 5 mM [Ca2+]o the inward TI declined to zero at about -30 mV, and test voltages between -55 and +5 mV failed to reveal a distinct outward TI.(ABSTRACT TRUNCATED AT 400 WORDS) PMID:1284077

77 FR 42774 - Notice of Public Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-20

... effectiveness in case processing at the Commission level. The Review Commission encourages its stakeholders and... Commission (Review Commission) invites the public to a meeting on enhancing efficiency in case processing at the Commission level. Date and Time: August 30, 2012, from 10:30 a.m. until 12:30 p.m. ADDRESSES...

Association of Metformin Use With Risk of Lactic Acidosis Across the Range of Kidney Function: A Community-Based Cohort Study.

PubMed

Lazarus, Benjamin; Wu, Aozhou; Shin, Jung-Im; Sang, Yingying; Alexander, G Caleb; Secora, Alex; Inker, Lesley A; Coresh, Josef; Chang, Alex R; Grams, Morgan E

2018-06-04

Approximately 1 million patients in the United States with type 2 diabetes mellitus and mild-to-moderate kidney disease do not receive guideline-directed therapy with metformin. This may reflect uncertainty regarding the risk of acidosis in patients with chronic kidney disease. To quantify the association between metformin use and hospitalization with acidosis across the range of estimated glomerular filtration rate (eGFR), accounting for change in eGFR stage over time. Community-based cohort of 75 413 patients with diabetes in Geisinger Health System, with time-dependent assessment of eGFR stage from January 2004 until January 2017. Results were replicated in 67 578 new metformin users and 14 439 new sulfonylurea users from 2010 to 2015, sourced from 350 private US health systems. Metformin use. Hospitalization with acidosis (International Classification of Diseases, Ninth Revision, Clinical Modification code of 276.2). In the primary cohort (n = 75 413), mean (SD) patient age was 60.4 (15.5) years, and 51% (n = 38 480) of the participants were female. There were 2335 hospitalizations with acidosis over a median follow-up of 5.7 years (interquartile range, 2.5-9.9 years). Compared with alternative diabetes management, time-dependent metformin use was not associated with incident acidosis overall (adjusted hazard ratio [HR], 0.98; 95% CI, 0.89-1.08) or in patients with eGFR 45 to 59 mL/min/1.73 m2 (adjusted HR, 1.16; 95% CI, 0.95-1.41) and eGFR 30 to 44 mL/min/1.73 m2 (adjusted HR, 1.09; 95% CI, 0.83-1.44). On the other hand, metformin use was associated with an increased risk of acidosis at eGFR less than 30 mL/min/1.73 m2 (adjusted HR, 2.07; 95% CI, 1.33-3.22). Results were consistent when new metformin users were compared with new sulfonylurea users (adjusted HR for eGFR 30-44 mL/min/1.73 m2, 0.77; 95% CI, 0.29-2.05), in a propensity-matched cohort (adjusted HR for eGFR 30-44 mL/min/1.73 m2, 0.71; 95% CI, 0.45-1.12), when baseline insulin users were excluded (adjusted HR for eGFR 30-44 mL/min/1.73 m2, 1.16; 95% CI, 0.87-1.57), and in the replication cohort (adjusted HR for eGFR 30-44 mL/min/1.73 m2, 0.86; 95% CI, 0.37-2.01). In 2 real-world clinical settings, metformin use was associated with acidosis only at eGFR less than 30 mL/min/1.73 m2. Our results support cautious use of metformin in patients with type 2 diabetes and eGFR of at least 30 mL/min/1.73 m2.

Dog and Cat Interactions in a Remote Aboriginal Community.

PubMed

Kennedy, Brooke; Brown, Wendy Y; Vernes, Karl; Körtner, Gerhard; Butler, James R A

2018-04-26

This study examined dog and cat demographics, roaming behaviours, and interspecific interactions in a remote Aboriginal island community using multiple methods. Our results revealed temporal differences between the roaming behaviours of dogs, cats, and wildlife. Dogs showed crepuscular behaviour, being active around dawn (5:30 a.m. to 9:30 a.m.) and dusk (6:00 p.m. and 11:35 p.m.). The majority of cats were active between dawn (6:30 a.m.) and dusk (7:30 p.m.) and travelled shorter distances than dogs. However, some cats were also observed roaming between dusk and dawn, and were likely to be hunting since flightless wildlife were also recorded on our remote-sensing cameras during this time. These baseline data provide evidence to suggest that new management programs are needed to reduce the number of roaming cats and therefore their potential impacts on native wildlife. Collaborations between Aboriginal owners and other stakeholders is necessary to design innovative and effective animal management and policy on the island.

2,4,6-Trichlorophenylhydrazine Schiff bases as DPPH radical and super oxide anion scavengers.

PubMed

Khan, Khalid Mohammed; Shah, Zarbad; Ahmad, Viqar Uddin; Khan, Momin; Taha, Muhammad; Rahim, Fazal; Ali, Sajjad; Ambreen, Nida; Perveen, Shahnaz; Choudhary, M Iqbal; Voelter, Wolfgang

2012-05-01

Syntheses of thirty 2,4,6-trichlorophenylhydrazine Schiff bases 1-30 were carried out and evaluated for their in vitro DPPH radical and super oxide anion scavenging activities. Compounds 1-30 have shown a varying degree of DPPH radical scavenging activity and their IC50 values range between 4.05-369.30 µM. The compounds 17, 28, 18, 14, 8, 15, 12, 2, 29, and 7 exhibited IC50 values ranging between 4.05±0.06-24.42±0.86 µM which are superior to standard n-propylgallate (IC50=30.12±0.27 µM). Selected compounds have shown a varying degree of superoxide anion radical scavenger activity and their IC50 values range between 91.23-406.90 µM. The compounds 28, 8, 17, 15, and 14, showed IC50 values between 91.23±1.2-105.31±2.29 µM which are superior to standard n-propylgallate (IC50=106.34±1.6 µM).

[Clinicopathologic features of primary mucosal CD30-positive T-cell lymphoproliferative disorders in head and neck region].

PubMed

Liu, F; Li, M; Zhang, L Y; Guo, L; Hu, W W; Rao, H L

2018-06-08

Objective: To study clinicopathologic features, prognosis and differential diagnoses of primary mucosal CD30-positive T-cell lymphoproliferative disorders of the head and neck(mCD30(+) TLPD-head and neck). Methods: Three cases of mCD30(+) TLPD-head and neck were collected from January 2014 to April 2017 at Sun Yat-Sen University Foshan Hospital. A literature review of mCD30(+) TLPD of head and neck was provided. Results: All three cases presented with either bulging/exophytic nodule or mucosal ulcer/erosion. Morphologically, the tumor consisted of diffuse proliferation of uniform, large atypical mononuclear lymphoid cells that showed irregular or polymorphic nuclei with small nucleoli, and abundant pale or amphophilic cytoplasm. Hallmark cells with eccentric, horseshoe, kidney-like, or doughnut-shaped nuclei were present. While mitotic figures were present, no tumor necrosis was found. Eosinophilc infiltration was obvious in the background. The atypical large lymphoid cells had a immunophenotype of CD30(+) /CD3(+) /CD4(+) /CD56(-) along with positive cytotoxic molecule. While being negative for EBER/ALK/CD20/CD8, TCR rearrangement was found in 2 out of 3 cases. Three patients were cured after excision without relapse and metastasis.The two patients with TCR rearrangement didn't show aggressive clinical course. Conclusions: mCD30(+) TLPD-head and neck is a rare benign lymphoproliferative disorder with spontaneous regression. It should be differentiated from cutaneous CD30(+) anaplstic large cell lymphoma, lymphomatoid papulosis, and EBV-related mucocutaneous ulcer. Correct recognition of mCD30(+) TLPD of head and neck is important to avoid overtreatment.

Is The Real In Vivo Nitric Oxide Concentration Pico or Nano Molar? Influence of Electrode Size on Unstirred Layers and NO Consumption

PubMed Central

Bohlen, H. Glenn

2012-01-01

Objective There is a debate if the nitric oxide concentration ([NO]) required to influence vascular smooth muscle is below 50 nM or much higher. 30 μm and larger diameter electrodes report [NO] below 50 nM, whereas diameters of < 10–12 μm report hundreds of nM. This study examined how size of electrodes influenced [NO] measurement due to NO consumption and unstirred layer issues. Methods Electrodes were 2 mm disk, 30μm X 2 mm carbon fiber, and single 7μm diameter carbon fiber within open tip microelectrode, and exposed 7 μm carbon fiber of ~15 μm to 2 mm length. Results All electrodes demonstrated linear calibrations with sufficient stirring. As stirring slowed, 30 μm and 2 mm electrodes reported much lower [NO] due to unstirred layers and high NO consumption. The three 7 μm microelectrodes had minor stirring issues. With limited stirring with NO present, 7 μm open tip microelectrodes advanced toward 30 μm and 2 mm electrodes experienced dramatically decreased current within 10–50μm of the larger electrodes due to high NO consumption. None of the 7 μm microelectrodes interacted. Conclusions The data indicate large electrodes underestimate [NO] due to excessive NO consumption under conditions where unstirred layers are unavoidable and true microelectrodes are required for valid measurements. PMID:22925222

Relaxivity of Ferumoxytol at 1.5 T and 3.0 T.

PubMed

Knobloch, Gesine; Colgan, Timothy; Wiens, Curtis N; Wang, Xiaoke; Schubert, Tilman; Hernando, Diego; Sharma, Samir D; Reeder, Scott B

2018-05-01

The aim of this study was to determine the relaxation properties of ferumoxytol, an off-label alternative to gadolinium-based contrast agents, under physiological conditions at 1.5 T and 3.0 T. Ferumoxytol was diluted in gradually increasing concentrations (0.26-4.2 mM) in saline, human plasma, and human whole blood. Magnetic resonance relaxometry was performed at 37°C at 1.5 T and 3.0 T. Longitudinal and transverse relaxation rate constants (R1, R2, R2*) were measured as a function of ferumoxytol concentration, and relaxivities (r1, r2, r2*) were calculated. A linear dependence of R1, R2, and R2* on ferumoxytol concentration was found in saline and plasma with lower R1 values at 3.0 T and similar R2 and R2* values at 1.5 T and 3.0 T (1.5 T: r1saline = 19.9 ± 2.3 smM; r1plasma = 19.0 ± 1.7 smM; r2saline = 60.8 ± 3.8 smM; r2plasma = 64.9 ± 1.8 smM; r2*saline = 60.4 ± 4.7 smM; r2*plasma = 64.4 ± 2.5 smM; 3.0 T: r1saline = 10.0 ± 0.3 smM; r1plasma = 9.5 ± 0.2 smM; r2saline = 62.3 ± 3.7 smM; r2plasma = 65.2 ± 1.8 smM; r2*saline = 57.0 ± 4.7 smM; r2*plasma = 55.7 ± 4.4 smM). The dependence of relaxation rates on concentration in blood was nonlinear. Formulas from second-order polynomial fittings of the relaxation rates were calculated to characterize the relationship between R1blood and R2 blood with ferumoxytol. Ferumoxytol demonstrates strong longitudinal and transverse relaxivities. Awareness of the nonlinear relaxation behavior of ferumoxytol in blood is important for ferumoxytol-enhanced magnetic resonance imaging applications and for protocol optimization.

33 CFR 165.T01-0477 - Safety Zones; Fireworks Displays in Captain of the Port Long Island Sound Zone.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Fireworks • Date: June 23, 2012.• Rain Date: June 24, 2012. • Time: 8:30 p.m. to 10:30 p.m. • Location: A...°18′21.92″ W (NAD 83). 2Salute to Veterans Fireworks • Date: June 23, 2012.• Rain Date: June 30, 2012...″ W (NAD 83). 3Goren Wedding Fireworks • Date: June 30, 2012.• Rain Date: July 1, 2012. • Time: 8:30 p...

33 CFR 117.821 - Atlantic Intracoastal Waterway, Albermarle Sound to Sunset Beach.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., (Newport River) at Morehead City, shall operate as follows: (1) During the day from 5 a.m. to 8:30 p.m... delay the required opening up to 10 minutes past the hour or half hour. (c) From 5 a.m. on October 17... and to perform periodic maintenance. (2) At night, need not open 8:30 p.m. to 5 a.m. except an opening...

Inventory of File sref_em.t03z.pgrb212.p1.f00.grib2

Science.gov Websites

Relative Humidity [%] 014.1 10 m above ground UGRD analysis U-Component of Wind [m/s] 014.2 10 m above ground VGRD analysis V-Component of Wind [m/s] 015 surface WEASD analysis Water Equivalent of Accumulated Wind [m/s] 032.2 30-0 mb above ground VGRD analysis V-Component of Wind [m/s] 033 30-0 mb above ground

Inventory of File sref_em.t03z.pgrb221.p1.f00.grib2

Science.gov Websites

Relative Humidity [%] 014.1 10 m above ground UGRD analysis U-Component of Wind [m/s] 014.2 10 m above ground VGRD analysis V-Component of Wind [m/s] 015 surface WEASD analysis Water Equivalent of Accumulated Wind [m/s] 032.2 30-0 mb above ground VGRD analysis V-Component of Wind [m/s] 033 30-0 mb above ground

Vs30 mapping at selected sites within the Greater Accra Metropolitan Area

NASA Astrophysics Data System (ADS)

Nortey, Grace; Armah, Thomas K.; Amponsah, Paulina

2018-06-01

A large part of Accra is underlain by a complex distribution of shallow soft soils. Within seismically active zones, these soils hold the most potential to significantly amplify seismic waves and cause severe damage, especially to structures sited on soils lacking sufficient stiffness. This paper presents preliminary site classification for the Greater Accra Metropolitan Area of Ghana (GAMA), using experimental data from two-dimensional (2-D) Multichannel Analysis of Surface Wave (MASW) technique. The dispersive characteristics of fundamental mode Rayleigh type surface waves were utilized for imaging the shallow subsurface layers (approx. up to 30 m depth) by estimating the 1D (depth) and 2D (depth and surface location) shear wave velocities at 5 selected sites. The average shear wave velocity for 30 m depth (Vs30), which is critical in evaluating the site response of the upper 30 m, was estimated and used for the preliminary site classification of the GAM area, as per NEHRP (National Earthquake Hazards Reduction Program). Based on the Vs30 values obtained in the study, two common site types C, and D corresponding to shallow (>6 m < 30 m) weathered rock and deep (up 30 m thick) stiff soils respectively, have been identified within the study area. Lower velocity profiles are inferred for the residual soils (sandy to silty clays), derived from the Accraian Formation that lies mainly within Accra central. Stiffer soil sites lie to the north of Accra, and to the west near Nyanyano. The seismic response characteristics over the residual soils in the GAMA have become apparent using the MASW technique. An extensive site effect map and a more robust probabilistic seismic hazard analysis can now be efficiently built for the metropolis, by considering the site classes and design parameters obtained from this study.

Comparison of step-by-step kinematics in repeated 30m sprints in female soccer players.

PubMed

van den Tillaar, Roland

2018-01-04

The aim of this study was to compare kinematics in repeated 30m sprints in female soccer players. Seventeen subjects performed seven 30m sprints every 30s in one session. Kinematics were measured with an infrared contact mat and laser gun, and running times with an electronic timing device. The main findings were that sprint times increased in the repeated sprint ability test. The main changes in kinematics during the repeated sprint ability test were increased contact time and decreased step frequency, while no change in step length was observed. The step velocity increased in almost each step until the 14, which occurred around 22m. After this, the velocity was stable until the last step, when it decreased. This increase in step velocity was mainly caused by the increased step length and decreased contact times. It was concluded that the fatigue induced in repeated 30m sprints in female soccer players resulted in decreased step frequency and increased contact time. Employing this approach in combination with a laser gun and infrared mat for 30m makes it very easy to analyse running kinematics in repeated sprints in training. This extra information gives the athlete, coach and sports scientist the opportunity to give more detailed feedback and help to target these changes in kinematics better to enhance repeated sprint performance.

North Pacific Ocean. Subtropical Front North of Hawaii, R/V Thomas G. Thompson: 7 January - 10 February 1974. CTD Data Report,

DTIC Science & Technology

1987-01-01

3 140 421.7 18.9 05 pM 74 318 a&15.0 N 153 30. 0 W 5894 1016.2 5 190 M2.1 19.2 06pM 74 320 28 0.0ON 153 31.0 W 5349 10.4.0 a ISO 21. 7 18.9 06 PW 74...1018.7r 13 ISO 32.8 19.4 ON pM V4 8M5 21 30.0 N 153 30. 0 W 4709 1018.4 21 70 23.1 30.0 06 pm 74 826 21 18.0 N 153 33.0 W 4987 1019.4 16 70 33.3 30.0 06...is4Go6107304a 1461 3 2 9 78. 7927 8444ll 7’ PC 31260 7’ 4G3 be6 Is’to 10946 0 14614 a67 ISO 0 𔄃 84 464 2’ a’s 83 :69 2’ 466 66 2 14 662 1:167 1 1461 6

Special Inspector General for Iraq Reconstruction. Quarterly Report to the United States Congress

DTIC Science & Technology

2007-10-30

InSPeCtoR GeneRaL FoR IRaQ ReConStRUCtIon Fu nd in g M ec ha ni sm Co ns o li da te d Ap pr o pr ia ti o ns Re so lu ti o n, 2 00 3 Em er ge nc y W...8/25/2006) $30M (10/16/2006) $30M QRF (8/25/2006) $3.007B (7/13/2006) $30M FAD FAD RAD FADFAD OA FY 2006 P.L. 109-234 (6/15/2006) $3.007B ISFF...FAD Funding Authorization Document M Million OA Obligation Authority RAD Resource Allocation Document SF 132 Apportionment and Reapportionment

Identification of an Internal Ribosome Entry Segment in the 5′ Region of the Mouse VL30 Retrotransposon and Its Use in the Development of Retroviral Vectors

PubMed Central

López-Lastra, Marcelo; Ulrici, Sandrine; Gabus, Caroline; Darlix, Jean-Luc

1999-01-01

Mouse virus-like 30S RNAs (VL30m) constitute a family of retrotransposons, present at 100 to 200 copies, dispersed in the mouse genome. They display little sequence homology to Moloney murine leukemia virus (MoMLV), do not encode virus-like proteins, and have not been implicated in retroviral carcinogenesis. However, VL30 RNAs are efficiently packaged into MLV particles that are propagated in cell culture. In this study, we addressed whether the 5′ region of VL30m could replace the 5′ leader of MoMLV functionally in a recombinant vector construct. Our data confirm that the putative packaging sequence of VL30 is located within the 5′ region (nucleotides 362 to 1149 with respect to the cap structure) and that it can replace the packaging sequence of MoMLV. We also show that VL30m contains an internal ribosome entry segment (IRES) in the 5′ region, as do MoMLV, Friend murine leukemia virus, Harvey murine sarcoma virus, and avian reticuloendotheliosis virus type A. Our data show that both the packaging and IRES functions of the 5′ region of VL30m RNA can be efficiently used to develop retrotransposon-based vectors. PMID:10482590

Identification of an internal ribosome entry segment in the 5' region of the mouse VL30 retrotransposon and its use in the development of retroviral vectors.

PubMed

López-Lastra, M; Ulrici, S; Gabus, C; Darlix, J L

1999-10-01

Mouse virus-like 30S RNAs (VL30m) constitute a family of retrotransposons, present at 100 to 200 copies, dispersed in the mouse genome. They display little sequence homology to Moloney murine leukemia virus (MoMLV), do not encode virus-like proteins, and have not been implicated in retroviral carcinogenesis. However, VL30 RNAs are efficiently packaged into MLV particles that are propagated in cell culture. In this study, we addressed whether the 5' region of VL30m could replace the 5' leader of MoMLV functionally in a recombinant vector construct. Our data confirm that the putative packaging sequence of VL30 is located within the 5' region (nucleotides 362 to 1149 with respect to the cap structure) and that it can replace the packaging sequence of MoMLV. We also show that VL30m contains an internal ribosome entry segment (IRES) in the 5' region, as do MoMLV, Friend murine leukemia virus, Harvey murine sarcoma virus, and avian reticuloendotheliosis virus type A. Our data show that both the packaging and IRES functions of the 5' region of VL30m RNA can be efficiently used to develop retrotransposon-based vectors.

75 FR 10309 - Announcement of National Geospatial Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-05

... Geospatial Advisory Committee (NGAC) will meet on March 24-25, 2010 at the One Washington Circle Hotel, 1... a.m. to 5 p.m. on March 24 and from 8:30 a.m. to 4:30 p.m. on March 25. FOR FURTHER INFORMATION... Guidance/NGAC Action Plan The meeting will include an opportunity for public comment on March 25. Comments...

77 FR 7594 - Advisory Committee on Infant Mortality; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-13

... Infant Mortality (ACIM). Dates and Times: March 8, 2012, 8:30 a.m.-6 p.m.; March 9, 2012, 8:30 a.m.-3 p.m... mortality and improving the health status of infants and pregnant women; and factors affecting the continuum...; a Maternal and Child Health Bureau (MCHB) update; an update from the Committee's four workgroups...

76 FR 29772 - National Human Genome Research Institute; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-23

... Institute Special Emphasis Panel; ELSI-SEP. Date: June 21, 2011. Time: 8 a.m. to 5:30 p.m. Agenda: To review... Emphasis Panel; Data Analysis-SEP. Date: June 27, 2011. Time: 8 a.m. to 5:30 p.m. Agenda: To review and evaluate grant applications. Place: Courtyard Arlington Crystal City/Reagan National Airport, 2899...

33 CFR 117.622 - West Bay.

Code of Federal Regulations, 2014 CFR

2014-07-01

... a.m. to 6 p.m. (5) At all other times from May 1 through October 31, the draw shall open on signal..., at Osterville, shall operate as follows: (1) From November 1 through April 30, the draw shall open on... shall open on signal from 8 a.m. to 6 p.m. (3) From June 16 through September 30, the draw shall open on...

33 CFR 117.622 - West Bay.

Code of Federal Regulations, 2013 CFR

2013-07-01

... a.m. to 6 p.m. (5) At all other times from May 1 through October 31, the draw shall open on signal..., at Osterville, shall operate as follows: (1) From November 1 through April 30, the draw shall open on... shall open on signal from 8 a.m. to 6 p.m. (3) From June 16 through September 30, the draw shall open on...

33 CFR 117.622 - West Bay

Code of Federal Regulations, 2011 CFR

2011-07-01

... a.m. to 6 p.m. (5) At all other times from May 1 through October 31, the draw shall open on signal..., at Osterville, shall operate as follows: (1) From November 1 through April 30, the draw shall open on... shall open on signal from 8 a.m. to 6 p.m. (3) From June 16 through September 30, the draw shall open on...

Cannabinoid CB1 receptor and endothelium-dependent hyperpolarization in guinea-pig carotid, rat mesenteric and porcine coronary arteries

PubMed Central

Chataigneau, T; Félétou, M; Thollon, C; Villeneuve, N; Vilaine, J- P; Duhault, J; Vanhoutte, P M

1998-01-01

The purpose of these experiments was to determine whether or not the endothelium-dependent hyperpolarizations of the vascular smooth muscle cells (observed in the presence of inhibitors of nitric oxide synthase and cyclo-oxygenase) can be attributed to the production of an endogenous cannabinoid.Membrane potential was recorded in the guinea-pig carotid, rat mesenteric and porcine coronary arteries by intracellular microelectrodes.In the rat mesenteric artery, the cannabinoid receptor antagonist, SR 141716 (1 μM), did not modify either the resting membrane potential of smooth muscle cells or the endothelium-dependent hyperpolarization induced by acetylcholine (1 μM) (17.3±1.8 mV, n=4 and 17.8±2.6 mV, n=4, in control and presence of SR 141716, respectively). Anandamide (30 μM) induced a hyperpolarization of the smooth muscle cells (12.6±1.4 mV, n=13 and 2.0±3.0 mV, n=6 in vessels with and without endothelium, respectively) which could not be repeated in the same tissue, whereas acetylcholine was still able to hyperpolarize the preparation. The hyperpolarization induced by anandamide was not significantly influenced by SR 141716 (1 μM). HU-210 (30 μM), a synthetic CB1 receptor agonist, and palmitoylethanolamide (30 μM), a CB2 receptor agonist, did not influence the membrane potential of the vascular smooth muscle cells.In the rat mesenteric artery, the endothelium-dependent hyperpolarization induced by acetylcholine (1 μM) (19.0±1.7 mV, n=6) was not altered by glibenclamide (1 μM; 17.7±2.3 mV, n=3). However, the combination of charybdotoxin (0.1 μM) plus apamin (0.5 μM) abolished the acetylcholine-induced hyperpolarization and under these conditions, acetylcholine evoked a depolarization (7.7±2.7 mV, n=3). The hyperpolarization induced by anandamide (30 μM) (12.6±1.4 mV, n=13) was significantly inhibited by glibenclamide (4.0±0.4 mV, n=4) but not significantly affected by the combination of charybdotoxin plus apamin (17.3±2.3 mV, n=4).In the guinea-pig carotid artery, acetylcholine (1 μM) evoked endothelium-dependent hyperpolarization (18.8±0.7 mV, n=15). SR 141716 (10 nM to 10 μM), caused a direct, concentration-dependent hyperpolarization (up to 10 mV at 10 μM) and a significant inhibition of the acetylcholine-induced hyperpolarization. Anandamide (0.1 to 3 μM) did not influence the membrane potential. At a concentration of 30 μM, the cannabinoid agonist induced a non-reproducible hyperpolarization (5.6±1.3 mV, n=10) with a slow onset. SR 141716 (1 μM) did not affect the hyperpolarization induced by 30 μM anandamide (5.3±1.5 mV, n=3).In the porcine coronary artery, anandamide up to 30 μM did not hyperpolarize or relax the smooth muscle cells. The endothelium-dependent hyperpolarization and relaxation induced by bradykinin were not influenced by SR 141716 (1 μM).These results indicate that the endothelium-dependent hyperpolarizations, observed in the guinea-pig carotid, rat mesenteric and porcine coronary arteries, are not related to the activation of cannabinoid CB1 receptors. PMID:9535027

Identification and Targeting of Tyrosine Kinase Activity in Prostate Cancer Initiation, Progression, and Metastasis

DTIC Science & Technology

2013-12-01

University) "Effectors of the DNA damage and radiotherapy response in cancer" 9:20 pm - 9:30 pm Discussion TUESDAY 7:30 am - 8:30 am Breakfast 9:00...M. Morris , Hideki Onagi, Timothy M. Altamore, Allan B. Gamble, Christopher J. Easton Prohormone-substrate peptide sequence recognition by

77 FR 25207 - Advisory Committee for Social, Behavioral and Economic Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-27

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Social, Behavioral and Economic Sciences..., Behavioral and Economic Sciences ( 1171). Date/Time: May 17, 2012; 8:30 a.m. to 5:30 p.m.; May 18, 2012; 8:30..., Directorate for Social, Behavioral and Economic Sciences, National Science Foundation, 4201 Wilson Boulevard...

75 FR 25886 - Advisory Committee for Social, Behavioral, and Economic Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-10

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Social, Behavioral, and Economic Sciences..., Behavioral, and Economic Sciences ( 1171). Date/Time: May 20, 2010; 8:30 a.m. to 5:30 p.m. May 21, 2010; 8:30..., Directorate for Social, Behavioral, and Economic Sciences, National Science Foundation, 4201 Wilson Boulevard...

77 FR 61790 - Committee on Equal Opportunities in Science and Engineering (CEOSE); Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-11

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunities in Science and Engineering (CEOSE... Opportunities in Science and Engineering (1173). Dates/Time: October 30, 2012, 10 a.m.-5:30 p.m.; October 31... science and engineering. Agenda Tuesday, October 30, 2012 Opening Statement by the CEOSE Chair...

75 FR 33652 - Committee on Equal Opportunities in Science and Engineering (CEOSE); Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-14

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunities in Science and Engineering (CEOSE... Opportunities in Science and Engineering (1173). Dates/Time: June 29, 2010, 8:30 p.m.-5:30 p.m. June 30, 2010, 8... NSF concerning broadening participation in science and engineering. Agenda Monday, June 29, 2010...

mRNA bound to the 30S subunit is a HigB toxin substrate

PubMed Central

Schureck, Marc A.; Maehigashi, Tatsuya; Miles, Stacey J.; Marquez, Jhomar; Dunham, Christine M.

2016-01-01

Activation of bacterial toxins during stress results in cleavage of mRNAs in the context of the ribosome. These toxins are thought to function as global translational inhibitors yet recent studies suggest each may have distinct mRNA specificities that result in selective translation for bacterial survival. Here we demonstrate that mRNA in the context of a bacterial 30S subunit is sufficient for ribosome-dependent toxin HigB endonucleolytic activity, suggesting that HigB interferes with the initiation step of translation. We determined the X-ray crystal structure of HigB bound to the 30S, revealing that two solvent-exposed clusters of HigB basic residues directly interact with 30S 16S rRNA helices 18, 30, and 31. We further show that these HigB residues are essential for ribosome recognition and function. Comparison with other ribosome-dependent toxins RelE and YoeB reveals that each interacts with similar features of the 30S aminoacyl (A) site yet does so through presentation of diverse structural motifs. PMID:27307497

Levels of soluble CD30 in cord blood and peripheral blood during childhood are not correlated with the development of atopic disease or a family history of atopy.

PubMed

Holmlund, U; Bengtsson, A; Nilsson, C; Kusoffsky, E; Lilja, G; Scheynius, A; Sverremark-Ekström, E

2003-11-01

The CD30 molecule has been linked to Th2 responses. Furthermore, elevated levels of the soluble form of CD30 (sCD30) in blood as well as of the expression of CD30 on the plasma membrane of T cells are associated with atopic disease. To assess the potential usefulness of sCD30 levels as a prognostic indicator of and/or diagnostic marker for the development of atopic disease in children. sCD30 levels in cord blood and peripheral blood from 36 2-year-old (10 atopic and 26 non-atopic) and 74 7-year-old (35 atopic and 39 non-atopic) children were determined employing an ELISA procedure. Atopy was diagnosed on the basis of clinical evaluation in combination with a positive skin prick test. No significant correlation between sCD30 levels in cord blood and the development of atopic disease at 2 or 7 years of age was observed. At 7 years of age, the circulating sCD30 levels in children with atopic disease (median 41 U/mL, range 6-503 U/mL) did not differ from the corresponding values for non-atopic subjects (median 41 U/mL, range 8-402 U/mL). The same was true for children at 2 years of age. Furthermore, the sCD30 levels of children who had developed atopic eczema/dermatitis syndrome by the age of 7 years (median 49 U/mL, range 14-503 U/mL) were not significantly elevated in comparison with those of the non-atopic children. Finally, neither sCD30 levels in cord blood nor peripheral blood at 2 or 7 years of age could be linked to a family history of atopy. These findings indicate that the sCD30 concentration in cord blood is not a reliable prognostic indicator of, nor a useful diagnostic marker for, atopic disease in children up to 7 years of age. If such correlations do exist, they might be masked by age-dependent variations in the circulating levels of sCD30, which may reflect individual differences in the maturation of children's immunological responses.

Plasma oxalate levels in prevalent hemodialysis patients and potential implications for ascorbic acid supplementation.

PubMed

Liu, Yuguan; Weisberg, Lawrence S; Langman, Craig B; Logan, Amanda; Hunter, Krystal; Prasad, Deepali; Avila, Jose; Venkatchalam, Thaliga; Berns, Jeffrey S; Handelman, Garry J; Sirover, William D

2016-10-01

Ascorbic acid (AA) supplementation may increase hemoglobin levels and decrease erythropoiesis-stimulating agent dose requirement in patients with end stage renal disease (ESRD). While plasma AA levels >100μM may be supratherapeutic, levels of at least 30μM may be needed to improve wound healing and levels may need to reach 70μM to optimize erythropoiesis. Of concern, oxalate (Ox), an AA metabolite, can accumulate in ESRD. Historically, if plasma Ox levels remain ≥30μM, oxalosis was of concern. Contemporary hemodialysis (HD) efficiencies may decrease the risk of oxalosis by maintaining pre-HD Ox levels <30μM. This study focuses on the plasma Ox levels in HD patients. A prospective, observational study of 197 HD patients with pre-HD AA levels and pre-HD and post-HD Ox levels. Mean plasma Ox levels decreased 71% during the intradialytic period (22.3±11.1μM to 6.4±3.2μM, P<0.001). In regression analysis, pre-HD plasma AA levels ≤100μM were not associated with a pre-HD plasma Ox level≥30μM, even if ferritin levels were increased. Pre-HD plasma Ox levels ≥20 or ≥30μM were not associated with lower cumulative 4-year survival. Pre-HD plasma AA levels up to 100μM in HD patients do not appear to be associated with an increased risk of developing secondary oxalosis, as the corresponding pre-HD plasma Ox level appears to be maintained at tolerable levels. Copyright © 2016 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

75 FR 57537 - Sunshine Act; Notice of Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-21

..., 2010 9:30 a.m. Briefing on Security Issues (Closed--Ex. 1). * * * * * * The schedule for Commission..., 2010 1 p.m. Briefing on Resolution of Generic Safety Issue (GSI)--191, Assessment of Debris... 18, 2010 1:30 p.m. NRC All Employees Meeting (Public Meeting) Marriott Bethesda North Hotel, 5701...

48 CFR 515.506 - Postaward debriefing of offerors.

Code of Federal Regulations, 2010 CFR

2010-10-01

... CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Preaward, Award, and Postaward Notifications... receipt of a request for a post award debriefing, GSA's hours of operation are 8:00 a.m. to 4:30 p.m. Request received after 4:30 p.m. will be considered received the following business day. ...

48 CFR 515.506 - Postaward debriefing of offerors.

Code of Federal Regulations, 2011 CFR

2011-10-01

... CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Preaward, Award, and Postaward Notifications... receipt of a request for a post award debriefing, GSA's hours of operation are 8:00 a.m. to 4:30 p.m. Request received after 4:30 p.m. will be considered received the following business day. ...

The USAF Stability and Control Digital DATCOM. Volume I. Users Manual

DTIC Science & Technology

1979-04-01

8217 Increment in lift due to thrust, 13 Eqn. 4.6.1-c 21 XBARP Y 13 22 DEUDA 3e /3a( 4.6.1 Eqn. 4.6.i-m 13: ~u 23-42 DCLNP (ACL)Np 4.6.1 Eqn. 4.6.1-I 13 43-62...T) 30 22 XBARIN XIN 30 23 XIN0CR IN/Cr 30 24 DEUDA ac /a 4.4.1 Eqn. 4.6.1-m 30u 25 EPSLON 30 26 ATJ (cT)j 4.6.1 Eqn. 4.6.1-a 30 27-46 DCLNJ (ACL)N

Inventory of File gfs.t06z.pgrb2.0p25.anl

Science.gov Websites

UGRD analysis U-Component of Wind [m/s] 005 10 mb VGRD analysis V-Component of Wind [m/s] 006 10 mb -Component of Wind [m/s] 011 20 mb VGRD analysis V-Component of Wind [m/s] 012 20 mb ABSV analysis Absolute UGRD analysis U-Component of Wind [m/s] 018 30 mb VGRD analysis V-Component of Wind [m/s] 019 30 mb

Inventory of File gfs.t06z.pgrb2.0p50.anl

Science.gov Websites

UGRD analysis U-Component of Wind [m/s] 005 10 mb VGRD analysis V-Component of Wind [m/s] 006 10 mb -Component of Wind [m/s] 011 20 mb VGRD analysis V-Component of Wind [m/s] 012 20 mb ABSV analysis Absolute UGRD analysis U-Component of Wind [m/s] 018 30 mb VGRD analysis V-Component of Wind [m/s] 019 30 mb

Inventory of File sref_nmb.t03z.pgrb212.p1.f00.grib2

Science.gov Websites

Relative Humidity [%] 014.1 10 m above ground UGRD analysis U-Component of Wind [m/s] 014.2 10 m above ground VGRD analysis V-Component of Wind [m/s] 015 surface WEASD analysis Water Equivalent of Accumulated Relative Humidity [%] 033.1 30-0 mb above ground UGRD analysis U-Component of Wind [m/s] 033.2 30-0 mb

Inventory of File sref_nmm.t03z.pgrb212.p1.f00.grib2

Science.gov Websites

Relative Humidity [%] 014.1 10 m above ground UGRD analysis U-Component of Wind [m/s] 014.2 10 m above ground VGRD analysis V-Component of Wind [m/s] 015 surface WEASD analysis Water Equivalent of Accumulated Relative Humidity [%] 033.1 30-0 mb above ground UGRD analysis U-Component of Wind [m/s] 033.2 30-0 mb

Effects of long-term treatment with the luteinizing hormone-releasing hormone (LHRH) agonist Decapeptyl and the LHRH antagonist Cetrorelix on the levels of pituitary LHRH receptors and their mRNA expression in rats

PubMed Central

Horvath, Judit E.; Bajo, Ana M.; Schally, Andrew V.; Kovacs, Magdolna; Herbert, Francine; Groot, Kate

2002-01-01

The effects of depot formulations of the luteinizing hormone-releasing hormone (LHRH) agonist Decapeptyl (25 μg/day) for 30 days or LHRH antagonist Cetrorelix pamoate (100 μg/day) for 30 days and daily injections of 100 μg of Decapeptyl for 10 days on the expression of mRNA for pituitary LHRH receptor (LHRH-R) and the levels of LHRH-R protein were evaluated in rats. Serum sex steroid concentrations and the weights of the reproductive organs were greatly reduced in all groups treated with analogs, demonstrating an efficient blockade of the pituitary–gonadal axis. Decapeptyl microcapsules elevated serum LH in female rats, but decreased it in male rats. LHRH-R mRNA expression in female pituitaries was reduced to 41% and 56–65% on days 10 and 30, respectively, whereas LHRH-R protein was 64% of control on day 10 and returned to pretreatment levels on day 30. Decapeptyl microcapsules reduced LHRH-R mRNA expression in male pituitaries to 58% on day 30 but not LHRH-R protein. Daily injections of Decapeptyl caused a desensitization of LH responses in female rats, while raising LHRH-R mRNA expression in female rats by 23% and LHRH-R protein levels by 119%. Cetrorelix pamoate reduced serum LH in female rats and diminished LHRH-R mRNA to 30% and 26% and LHRH-R protein to 57% and 48% on days 10 and 30, respectively. Elevated LHRH-R protein levels of ovariectomized rats were reduced after 10-day treatment with Cetrorelix or 100 μg/day Decapeptyl. Thus, changes in the mRNA expression after treatment with Cetrorelix, but not always Decapeptyl, paralleled those of LHRH-R protein. The inhibitory effect of Cetrorelix on serum LH, pituitary LHRH-R mRNA, and LHRH-R protein was greater than that of Decapeptyl. PMID:12409615

Modeling and Simulation of Ceramic Arrays to Improve Ballaistic Performance

DTIC Science & Technology

2013-09-09

targets with .30cal AP M2 projectile using SPH elements. -Model validation runs were conducted based on the DoP experiments described in reference...effect of material properties on DoP 15. SUBJECT TERMS .30cal AP M2 Projectile, 762x39 PS Projectile, SPH , Aluminum 5083, SiC, DoP Expeminets...and ceramic-faced aluminum targets with „30cal AP M2 projectile using SPH elements. □ Model validation runs were conducted based on the DoP

Carbon and Nitrogen Mineralization in Relation to Soil Particle-Size Fractions after 32 Years of Chemical and Manure Application in a Continuous Maize Cropping System.

PubMed

Cai, Andong; Xu, Hu; Shao, Xingfang; Zhu, Ping; Zhang, Wenju; Xu, Minggang; Murphy, Daniel V

2016-01-01

Long-term manure application is recognized as an efficient management practice to enhance soil organic carbon (SOC) accumulation and nitrogen (N) mineralization capacity. A field study was established in 1979 to understand the impact of long-term manure and/or chemical fertilizer application on soil fertility in a continuous maize cropping system. Soil samples were collected from field plots in 2012 from 9 fertilization treatments (M0CK, M0N, M0NPK, M30CK, M30N, M30NPK, M60CK, M60N, and M60NPK) where M0, M30, and M60 refer to manure applied at rates of 0, 30, and 60 t ha(-1) yr(-1), respectively; CK indicates no fertilizer; N and NPK refer to chemical fertilizer in the forms of either N or N plus phosphorus (P) and potassium (K). Soils were separated into three particle-size fractions (2000-250, 250-53, and <53 μm) by dry- and wet-sieving. A laboratory incubation study of these separated particle-size fractions was used to evaluate the effect of long-term manure, in combination with/without chemical fertilization application, on the accumulation and mineralization of SOC and total N in each fraction. Results showed that long-term manure application significantly increased SOC and total N content and enhanced C and N mineralization in the three particle-size fractions. The content of SOC and total N followed the order 2000-250 μm > 250-53 μm > 53 μm fraction, whereas the amount of C and N mineralization followed the reverse order. In the <53 μm fraction, the M60NPK treatment significantly increased the amount of C and N mineralized (7.0 and 10.1 times, respectively) compared to the M0CK treatment. Long-term manure application, especially when combined with chemical fertilizers, resulted in increased soil microbial biomass C and N, and a decreased microbial metabolic quotient. Consequently, long-term manure fertilization was beneficial to both soil C and N turnover and microbial activity, and had significant effect on the microbial metabolic quotient.

M cell-targeting strategy facilitates mucosal immune response and enhances protection against CVB3-induced viral myocarditis elicited by chitosan-DNA vaccine.

PubMed

Ye, Ting; Yue, Yan; Fan, Xiangmei; Dong, Chunsheng; Xu, Wei; Xiong, Sidong

2014-07-31

Efficient delivery of antigen to mucosal associated lymphoid tissue is a first and critical step for successful induction of mucosal immunity by vaccines. Considering its potential transcytotic capability, M cell has become a more and more attractive target for mucosal vaccines. In this research, we designed an M cell-targeting strategy by which mucosal delivery system chitosan (CS) was endowed with M cell-targeting ability via conjugating with a CPE30 peptide, C terminal 30 amino acids of clostridium perfringens enterotoxin (CPE), and then evaluated its immune-enhancing ability in the context of coxsackievirus B3 (CVB3)-specific mucosal vaccine consisting of CS and a plasmid encoding CVB3 predominant antigen VP1. It had shown that similar to CS-pVP1, M cell-targeting CPE30-CS-pVP1 vaccine appeared a uniform spherical shape with about 300 nm diameter and +22 mV zeta potential, and could efficiently protect DNA from DNase I digestion. Mice were orally immunized with 4 doses of CPE30-CS-pVP1 containing 50 μg pVP1 at 2-week intervals and challenged with CVB3 4 weeks after the last immunization. Compared with CS-pVP1 vaccine, CPE30-CS-pVP1 vaccine had no obvious impact on CVB3-specific serum IgG level and splenic T cell immune responses, but significantly increased specific fecal SIgA level and augmented mucosal T cell immune responses. Consequently, much milder myocarditis and lower viral load were witnessed in CPE30-CS-pVP1 immunized group. The enhanced immunogenicity and immunoprotection were associated with the M cell-targeting ability of CPE30-CS-pVP1 which improved its mucosal uptake and transcytosis. Our findings indicated that CPE30-CS-pVP1 may represent a novel prophylactic vaccine against CVB3-induced myocarditis, and this M cell-targeting strategy indeed could be applied as a promising and universal platform for mucosal vaccine development. Copyright © 2014 Elsevier Ltd. All rights reserved.

VS Characterization of Hard-Rock DAM Sites in British Columbia

NASA Astrophysics Data System (ADS)

Addo, K. O.; Catchings, R.; Yong, A.; Goldman, M.; Chan, J. H.; Martin, A. J.

2017-12-01

We present results consisting of shear-wave velocity (VS) profiles and the time-averaged VS in the uppermost 30 m (VS30) measured with multiple noninvasive seismic methods and acquired at five hydro dam locations in British Columbia, Canada. VS30 is typically the main parameter used to account for site amplification in ground motion models (GMMs), including models for western (WNA) and central/eastern North America (CENA). As VS30 quantifies soil shear stiffness, which affects frequency content and damping within shallow sediments, it correlates with the shallow-crustal damping parameter, kappa (k), and particularly the site component of kappa (k0). The upper limit on k0-VS30-scaling is in the range of 1100 to 1500 m/s (or less) and the lack of data from stiffer sites reflects the scarcity of direct VS measurements for such site conditions in North America. Hard-rock sites (VS30 > 1500 m/s) are of great engineering interest, but the lack of such measurements increases epistemic uncertainties in the GMMs. Moreover, it is currently not possible to correlate site-to-site variations in k0 with VS30 for such conditions because most hard-rock sites are assigned a generic VS30 of 2000 m/s, due to the lack of measured VS30 values. For the British Columbia sites, our preliminary analysis of field records indicates near-surface shear-wave velocities in excess of 2500 m/s in the upper few meters. Additional analysis of body- and surface-waves will include: refraction tomography, multi-channel analysis of surface waves (MASW), reflection, extended-spatial-autocorrelation, horizontal-to-vertical spectral ratio, and multi-spectral analysis of surface waves

Genomic Analysis of Multidrug-Resistant Escherichia coli from North Carolina Community Hospitals: Ongoing Circulation of CTX-M-Producing ST131-H30Rx and ST131-H30R1 Strains.

PubMed

Kanamori, Hajime; Parobek, Christian M; Juliano, Jonathan J; Johnson, James R; Johnston, Brian D; Johnson, Timothy J; Weber, David J; Rutala, William A; Anderson, Deverick J

2017-08-01

Escherichia coli sequence type 131 (ST131) predominates globally among multidrug-resistant (MDR) E. coli strains. We used whole-genome sequencing (WGS) to investigate 63 MDR E. coli isolates from 7 North Carolina community hospitals (2010 to 2015). Of these, 39 (62%) represented ST131, including 37 (95%) from the ST131- H 30R subclone: 10 (27%) from its H 30R1 subset and 27 (69%) from its H 30Rx subset. ST131 core genomes differed by a median of 15 (range, 0 to 490) single-nucleotide variants (SNVs) overall versus only 7 within H 30R1 (range, 3 to 12 SNVs) and 11 within H 30Rx (range, 0 to 21). The four isolates with identical core genomes were all H 30Rx. Epidemiological and clinical characteristics did not vary significantly by strain type, but many patients with MDR E. coli or H 30Rx infection were critically ill and had poor outcomes. H 30Rx isolates characteristically exhibited fluoroquinolone resistance and CTX-M-15 production, had a high prevalence of trimethoprim-sulfamethoxazole resistance (89%), sul1 (89%), and dfrA17 (85%), and were enriched for specific virulence traits, and all qualified as extraintestinal pathogenic E. coli The high overall prevalence of CTX-M-15 appeared to be possibly attributable to its association with the ST131- H 30Rx subclone and IncF[F2:A1:B-] plasmids. Some phylogenetically clustered non-ST131 MDR E. coli isolates also had distinctive serotypes/ fimH types, fluoroquinolone mutations, CTX-M variants, and IncF types. Thus, WGS analysis of our community hospital source MDR E. coli isolates suggested ongoing circulation and differentiation of E. coli ST131 subclones, with clonal segregation of CTX-M variants, other resistance genes, Inc-type plasmids, and virulence genes. Copyright © 2017 American Society for Microbiology.

Post-transplant monitoring of soluble CD30 level as predictor of graft outcome: a single center experience from China.

PubMed

Wang, Dong; Wu, Weizhen; Yang, Shunliang; Wang, Qinghua; Tan, Jianming

2012-12-01

There are no reliable parameters for post-transplantation immunological monitoring, which might enable recipient-tailored immunosuppressive therapy. 250 renal graft recipients were enrolled and detected for sCD30 level pre-transplantation, and on days 5 and 14, and on months 1, 3, 6, 12, 24, 36, 48 and 60 post-transplantation. Analysis was performed on correlation between sCD30 level and acute rejection, lung infection, or graft loss respectively. sCD30 levels descended to a nadir with a mean of 10.2 ± 3.8 U/mL on day 30 post-transplantation, then rose gradually, and approached 21.8 ± 10.1 U/mL on month 3, 34.2 ± 16.5 U/mL on month 6, and 42.9 ± 29.5 U/mL on month 12, then presented a stable level. Recipients with AR had significantly higher sCD30 levels than those without AR on days 5 and 14 post-transplantation. Recipients with pneumonia had significantly lower sCD30 levels within 3 months post-transplantation than those without pneumonia. Significantly higher sCD30 levels were recorded in recipients who suffered graft loss than those with normal graft function on days 5 and 14, and on months 6, 12, and 24. High sCD30 level (≥ 48.3 U/mL) at month 12 post-transplantation has an obvious detrimental effect on renal graft survival (p=0.000, HR=9.075). Serum sCD30 level might reflect immune state of renal graft recipients. Post-transplantation sequential monitoring of sCD30 level is necessary, which might not only identify recipients at the risk of acute rejection and graft loss, but also chosen as an independent predictor of pneumonia in renal transplant recipients. Copyright © 2012 Elsevier B.V. All rights reserved.

Electronic Tongue Response to Chemicals in Orange Juice that Change Concentration in Relation to Harvest Maturity and Citrus Greening or Huanglongbing (HLB) Disease.

PubMed

Raithore, Smita; Bai, Jinhe; Plotto, Anne; Manthey, John; Irey, Mike; Baldwin, Elizabeth

2015-12-02

In an earlier study, an electronic tongue system (e-tongue) has been used to differentiate between orange juice made from healthy fruit and from fruit affected by the citrus greening or Huanglongbing (HLB) disease. This study investigated the reaction of an e-tongue system to the main chemicals in orange juice that impact flavor and health benefits and are also impacted by HLB. Orange juice was spiked with sucrose (0.2-5.0 g/100 mL), citric acid (0.1%-3.0% g/100 mL) and potassium chloride (0.1-3.0 g/100 mL) as well as the secondary metabolites nomilin (1-30 µg/mL), limonin (1-30 µg/mL), limonin glucoside (30-200 µg/mL), hesperidin (30-400 µg/mL) and hesperetin (30-400 µg/mL). Performance of Alpha MOS sensor sets #1 (pharmaceutical) and #5 (food) were compared for the same samples, with sensor set #1 generally giving better separation than sensor set #5 for sucrose, sensor set #5 giving better separation for nomilin and limonin, both sets being efficient at separating citric acid, potassium chloride, hesperitin and limonin glucoside, and neither set discriminating hesperidin efficiently. Orange juice made from fruit over the harvest season and from fruit harvested from healthy or HLB-affected trees were separated by harvest maturity, disease state and disease severity.

Impairment of endothelium-dependent relaxation of rat aortas by homocysteine thiolactone and attenuation by captopril.

PubMed

Liu, Yu-Hui; You, Yu; Song, Tao; Wu, Shu-Jing; Liu, Li-Ying

2007-08-01

To explore the effects of angiotensin-converting enzyme (ACE) inhibitors on endothelial dysfunction induced by homocysteine thiolactone (HTL). Both endothelium-dependent relaxation and nondependent relaxation of thoracic aortic rings in rats induced by acetylcholine (Ach) or sodium nitroprusside (SNP) and biochemical parameters including malondialdehyde (MDA) and nitric oxide (NO) were measured in rat isolated aorta. Exposure of aortic rings to HTL (3 to 30 mM) for 90 minutes made a significant inhibition of endothelium-dependent relaxation induced by Ach, decreased contents of NO, and increased MDA concentration in aortic tissue. After incubation of aortic rings with captopril (0.003 to 0.03 mM) attenuated the inhibition of endothelium-dependent relaxation (EDR) and significantly resisted the decrease of NO content and elevation of MDA concentration caused by HTL (30 mmol/L) in aortic tissues, a similarly protective effect was observed when the aortic rings were incubated with both N-acetylcysteine (0.05 mM). Treatment with enalaprilat (0.003 to 0.01 mM) made no significant difference with the HTL (30 mM) group regarding EDR, but enalaprilat (0.03 mM) and losartan (0.03 mM) could partly restore the EDR in response to HTL (30 mM). Captopril was more effective than enalaprilat and losartan in attenuation of the inhibition of on acetylcholine-stimulated aortic relaxation by HTL in the same concentration. Moreover, superoxide dismutase (SOD, 200 U/mL), which is a scavenger of superoxide anions, apocynin (0.03 mM), which is an inhibitor of NADPH oxidase, and l-Arginine (3 mmol/L), a precursor of nitric oxide (NO), could reduce HTL (30 mM)-induced inhibition of EDR. After pretreatment with not only the NO synthase inhibitor Nomega-nitro-l-arginine methyl ester (L-NAME, 0.01 mM) but also the free sulfhydryl group blocking agent p-hydroxymercurybenzoate (PHMB, 0.05 mM) could abolish the protection of captopril and N-acetylcysteine, respectively. These results suggest that mechanisms of endothelial dysfunction induced by HTL may include the decrease of NO and the generation of oxygen free radicals and that captopril can restore the inhibition of EDR induced by HTL in isolated rat aorta, which may be related to scavenging oxygen free radicals and may be sulfhydryl-dependent.

An Evaluation of Contaminant Flux Rates from Sediments of Sinclair Inlet, WA, Using a Benthic Flux Sampling Device

DTIC Science & Technology

1993-02-01

rral SMNI,0&T0M 4.10 70 4.10 4.30 Caofwla 0.30 ChLqbw Mkme - 4m Iw $1NIAl" 42)7 *1 am• am7 am 0.30 27003 a00 am7 Oki O:OYIE󈧏 OM3 Qltnbw Am - lt0040...c20 020 0.m IftrodlYr rib ~wmW~ AM 7IG40 a.W2 130516.4 .42 12 20 .02 a20 42 0am ISO 0a= RS*m rib Sommom 402 83 ta20 42 l6.m .42 020 220 020 HI d M...omm 0~ $11,2824-71 1230 TV 121 12 0 axI~ IMl Ow V~al" - 40.65 km S3F42001 ISO ft 0.00 Im5 000 I .50 1.50 0.00 aoM S~dGEcFr=.i 0,54 Chrbk~m led M󈧄 r2

76 FR 51054 - Notice of Utah's Resource Advisory Council (RAC) Conference Call Meetings on the Statewide Travel...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-17

..., 2011, from 3 p.m. until 4:30 p.m. (Mountain Time). The entire RAC will host a conference call on the same topic on September 15, 2011, from 11 a.m.-12:30 p.m. (Mountain Time). ADDRESSES: On September 8... meals are the responsibility of the participating public. Juan Palma, State Director. [FR Doc. 2011...

76 FR 11248 - Secretary's Advisory Committee on Human Research Protections; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-01

.... SUMMARY: Pursuant to Section 10(a) of the Federal Advisory Committee Act, Public Law 92-463, as amended (5.../sachrp/mtgings/index.html . DATES: The meeting will be held on Tuesday, March 8, 2011 from 8:30 a.m. until 5 p.m. and Wednesday, March 9, 2011 from 8:30 a.m. until 5 p.m. ADDRESSES: U.S. Department of...

77 FR 48453 - Connect America Fund; High-Cost Universal Service Support; Universal Service Reform-Mobility Fund

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2012-08-14

... from 8:00 a.m. to 4:30 p.m. Eastern Time (ET) Monday through Thursday or from 8:00 a.m. to 11:30 a.m... that are not Tribally-owned or controlled; and (5) Declines to adopt a series of performance... providers. The Commission determined that both the auction design and natural advantages of carriers with...

77 FR 67826 - Office of the Director, National Institutes of Health; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-14

... Committee Act, as amended (5 U.S.C. App.), notice is hereby given of a meeting of the Recombinant DNA... Committee: Recombinant DNA Advisory Committee Date: December 4, 2012. Time: 1:50 p.m. to 5:30 p.m. Date: December 5, 2012. Time: 8:30 a.m. to 3:00 p.m. Agenda: The NIH Recombinant DNA Advisory Committee (RAC...

78 FR 21197 - Advisory Committee on Structural Safety of Department of Veterans Affairs Facilities, Notice of...

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2013-04-09

...:30 a.m. until 12:30 p.m. on April 26. The meeting is open to the public. The purpose of the Committee... should be sent to Krishna K. Banga, Senior Structural Engineer, Facilities Standards Service, Office of..., 2012 (Tele-Conf.) meetings--10:00 a.m. Break for lunch--12:00 p.m. Structural and Fire-Safety sub...

76 FR 27375 - Medical Review Board Public Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-11

... Period 11:45A-12:45 p.m. Break for Lunch 12:45-2 p.m. Update on 2007 Evidence Report on Sleep Apnea; Overview of 2007 Medical Expert Panel Opinions on Sleep Apnea 2-2:30 p.m. Review on Previous MRB recommendations on Sleep Apnea 2:30 -4 p.m. Public Comment Period Breaks will be announced on meeting day and may...

STS-30 Commander David M. Walker during preflight press conference at JSC

NASA Technical Reports Server (NTRS)

1989-01-01

During preflight press conference, STS-30 Commander David M. Walker monitors a question from a news media representative. The event was held in the JSC Auditorium and Public Affairs Facility Bldg 2 briefing room. STS-30 mission will fly onboard Atlantis, Orbiter Vehicle (OV) 104, and is scheduled for an April 28 liftoff.

76 FR 10039 - National Institute of Diabetes and Digestive and Kidney Diseases; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-23

... Diabetes and Digestive and Kidney Diseases Special Emphasis Panel; Chronic Pelvic Pain Clinical Study. Date... Digestive and Kidney Diseases Special Emphasis Panel; Ulcerative Colitis Clinical Trials. Date: March 29... Diseases Special Emphasis Panel; Urology Clinical Trials. Date: March 30, 2011. Time: 2:30 p.m. to 3:30 p.m...

Pilot-scale gasification of woody biomass

Treesearch

Thomas Elder; Leslie H. Groom

2011-01-01

The gasification of pine and mixed-hardwood chips has been carried out in a pilot-scale system at a range of gas flow rates. Consuming ~17-30 kgh-1 of feedstock, the producer gas was composed of ~200 dm3 m-3 carbon monoxide, 12 dm3 m-3 carbon dioxide, 30 dm3 m-3 methane and 190 dm3 m-3 hydrogen, with an energy content of ~6 MJ m-3 for both feedstocks. It was found that...

Enhanced biocompatibility and osteogenic potential of mesoporous magnesium silicate/polycaprolactone/wheat protein composite scaffolds.

PubMed

Kang, Yun Gyeong; Wei, Jie; Shin, Ji Won; Wu, Yan Ru; Su, Jiacan; Park, Young Shik; Shin, Jung-Woog

2018-01-01

Successful bone tissue engineering using scaffolds is primarily dependent on the properties of the scaffold, including biocompatibility, highly interconnected porosity, and mechanical integrity. In this study, we propose new composite scaffolds consisting of mesoporous magnesium silicate (m_MS), polycaprolactone (PCL), and wheat protein (WP) manufactured by a rapid prototyping technique to provide a micro/macro porous structure. Experimental groups were set based on the component ratio: (1) WP0% (m_MS:PCL:WP =30:70:0 weight per weight; w/w); (2) WP15% (m_MS:PCL:WP =30:55:15 w/w); (3) WP30% (m_MS:PCL:WP =30:40:30 w/w). Evaluation of the properties of fabricated scaffolds indicated that increasing the amount of WP improved the surface hydrophilicity and biodegradability of m_MS/PCL/WP composites, while reducing the mechanical strength. Moreover, experiments were performed to confirm the biocompatibility and osteogenic differentiation of human mesenchymal stem cells (MSCs) according to the component ratio of the scaffold. The results confirmed that the content of WP affects proliferation and osteogenic differentiation of MSCs. Based on the last day of the experiment, ie, the 14th day, the proliferation based on the amount of DNA was the best in the WP30% group, but all of the markers measured by PCR were the most expressed in the WP15% group. These results suggest that the m_MS/PCL/WP composite is a promising candidate for use as a scaffold in cell-based bone regeneration.

High Dietary Fructose Intake on Cardiovascular Disease Related Parameters in Growing Rats.

PubMed

Yoo, SooYeon; Ahn, Hyejin; Park, Yoo Kyoung

2016-12-26

The objective of this study was to determine the effects of a high-fructose diet on cardiovascular disease (CVD)-related parameters in growing rats. Three-week-old female Sprague Dawley rats were randomly assigned to four experimental groups; a regular diet group (RD: fed regular diet based on AIN-93G, n = 8), a high-fructose diet group (30Frc: fed regular diet with 30% fructose, n = 8), a high-fat diet group (45Fat: fed regular diet with 45 kcal% fat, n = 8) or a high fructose with high-fat diet group (30Frc + 45Fat, fed diet 30% fructose with 45 kcal% fat, n = 8). After an eight-week treatment period, the body weight, total-fat weight, serum glucose, insulin, lipid profiles and pro-inflammatory cytokines, abdominal aortic wall thickness, and expressions of eNOS and ET-1 mRNA were analyzed. The result showed that total-fat weight was higher in the 30Frc, 45Fat, and 30Frc + 45Fat groups compared to the RD group ( p < 0.05). Serum triglyceride (TG) levels were highest in the 30Frc group than the other groups ( p < 0.05). The abdominal aorta of 30Frc, 45Fat, and 30Frc + 45Fat groups had higher wall thickness than the RD group ( p < 0.05). Abdominal aortic eNOS mRNA level was decreased in 30Frc, 45Fat, and 30Frc + 45Fat groups compared to the RD group ( p < 0.05), and also 45Fat and 30Frc + 45Fat groups had decreased mRNA expression of eNOS compared to the 30Frc group ( p < 0.05). ET-1 mRNA level was higher in 30Frc, 45Fat, and 30Frc + 45Fat groups than the RD group ( p < 0.05). Both high fructose consumption and high fat consumption in growing rats had similar negative effects on CVD-related parameters.

77 FR 32601 - Meeting of the Defense Advisory Committee on Women in the Services (DACOWITS)

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-01

... Sexual Assault Prevention and Response Office (SAPRO) on sexual assault prevention measures. Additionally...:30 p.m.; June 27, 2012, 8:30 a.m.-12 p.m. ADDRESSES: 300 Army Navy Drive, Arlington, VA 22202. FOR...--Summary of Installation Visits. --Briefing--Sexual Assault Prevention Measures, SAPRO. Wednesday, June 27...

75 FR 13313 - Advisory Committee for International Science and Engineering; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-19

... NATIONAL SCIENCE FOUNDATION Advisory Committee for International Science and Engineering; Notice... Science and Engineering ( 25104). Date/Time: April 19, 2010--8:30 a.m. to 5 p.m. April 20, 2010--8:30 a.m... Engineering. Agenda April 19, 2010 AM: Introductions and Updates--Presentation and Discussion of 2010...

77 FR 13367 - Advisory Committee for International Science and Engineering; Notice of Meeting

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2012-03-06

... NATIONAL SCIENCE FOUNDATION Advisory Committee for International Science and Engineering; Notice... Science and Engineering (25104). Date and Time: March 19, 2012, 8:30 a.m.-5 p.m. March 20, 2012, 8:30 a.m.... Type of Meeting: Open. Contact Person: Robert Webber, Office of International Science and Engineering...

78 FR 13384 - Advisory Committee for International Science and Engineering; Notice of Meeting

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2013-02-27

... NATIONAL SCIENCE FOUNDATION Advisory Committee for International Science and Engineering; Notice... Science and Engineering (25104). Date/Time: March 14, 2013 9:30 a.m.-5:00 p.m. March 15, 2013 8:30 a.m.-12... of International Science and Engineering, National Science Foundation, 4201 Wilson Blvd., Arlington...

77 FR 66193 - Board of Governors; Sunshine Act Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-02

... POSTAL SERVICE Board of Governors; Sunshine Act Meeting DATES AND TIMES: Wednesday, November 14, 2012, at 10:00 a.m.; Thursday, November 15, at 8:30 a.m. and 10:30 a.m. PLACE: Washington, DC, at U.S. Postal Service Headquarters, 475 L'Enfant Plaza SW., in the Benjamin Franklin Room. STATUS: Wednesday...

Evaluation of the 'liking' and 'wanting' properties of umami compound in rats.

PubMed

Uematsu, Akira; Tsurugizawa, Tomokazu; Kitamura, Akihiko; Ichikawa, Reiko; Iwatsuki, Ken; Uneyama, Hisayuki; Torii, Kunio

2011-03-28

Food reward is neurologically and psychologically divided into at least two properties; 'liking' and 'wanting'. Although umami taste enhances food palatability, the liking and wanting properties of umami taste, and the underlying neural mechanisms for these properties are not clear. Here, we compared sucrose (0, 10, 30, 120 and 480 mM) and monosodium l-glutamate (MSG; 0, 10, 30, 60 and 120 mM) solutions using a taste reactivity test to evaluate liking, and fixed/progressive-ratio operant licking tasks to evaluate wanting. To determine the underlying neural mechanisms, we also conducted systemic blockade of opioid receptors in both tests. In the taste reactivity test, the hedonic reactions to 30, 60 and 120 mM MSG were greater than those to water (0mM) but lower than those to 480 mM sucrose. In the operant task, the intake, number of licks, and breakpoint to MSG reached peaks at around 60mM but they were lower than those to 30-480 mM sucrose. The systemic naloxone treatment decreased the hedonic responses to MSG and sucrose, and reduced the incentive salience of MSG but not sucrose. These findings indicate that the hedonic response and incentive salience of MSG is lower than those of sucrose when compared at the maximum response and that the incentive salience of MSG is lower than sucrose even where the hedonic response is similar. The present study also suggest that the hedonic response and incentive salience of umami compound is modulated by brain opioid signaling. Copyright © 2011 Elsevier Inc. All rights reserved.

An extraterrestrial event at the tertiary-quaternary boundary

NASA Technical Reports Server (NTRS)

Peng, H.

1994-01-01

During the North Pacific Manganese Nodules Investigation in 1983, we collected a large-diameter core M14 (8 deg. 00.15 N, 176 deg, 10.65 W; 3991-m depth of water). This gravity core is 420 cm long in total. It is composed of calcureous ooze at 0-320 cm and siliceous ooze at 320-420 cm of depth. A great number of microtektites were found at 270-300 cm (average content is 15 microtektites per 100-g sediment). Because abundant microtektites are restricted to a 30-m-thick zone of deep-sea core, we called this zone the microtektite layer. The age of the sedimentary stratum containg microtektites is 2.14-2.30 m.y., corresponding to the Pliocene to Pleistocene Periods as indicated by paleomagnetic and paleontologic analysis. We can give the following two primary conclusions: (1) the core M14 microtektites are products of a major meteorite or asteroid impact 2.14-2.30 m.y. ago; and (2) the age of 2.14-2.30 m.y. is an important geological age; perhaps it is just a correct age for dividing the Tertiary/Quarternary boundary. This result is quite close to previous data.

Effects of 4 Weeks of β-Alanine Supplementation on Swim-Performance Parameters in Water Polo Players.

PubMed

Brisola, Gabriel Motta Pinheiro; Milioni, Fabio; Papoti, Marcelo; Zagatto, Alessandro Moura

2017-08-01

In water polo, several high-intensity efforts are performed, leading to the fatigue process due to accumulation of hydrogen ions, and thus β-alanine supplementation could be an efficient strategy to increase the intramuscular acid buffer. Purpose To investigate whether 4 wk of β-alanine supplementation enhances parameters related to water polo performance. Methods Twenty-two highly trained male water polo players of national level were randomly assigned to receive 28 d of either β-alanine or a placebo (4.8 g/d of the supplement in the first 10 d and 6.4 g/d in the final 18 d). The participants performed 30-s maximal tethered swimming (30TS), 200-m swimming (P200m), and 30-s crossbar jumps (30CJ) before and after the supplementation period. Results The β-alanine group presented significant increases in 30TS for mean force (P = .04; Δ = 30.5% ± 40.4%) and integral of force (P = .05; Δ = 28.0% ± 38.0%), as well as P200m (P = .05; Δ = -2.2% ± 2.6%), while the placebo group did not significantly differ for mean force (P = .13; Δ = 24.1% ± 33.7%), integral of force (P = .12; Δ = 24.3% ± 35.1%), or P200m (P = .10; Δ = -1.6% ± 3.8%). However, there was no significant group effect for any variable, and the magnitude-based-inference analysis showed unclear outcomes between groups (Cohen d ± 95%CL mean force = 0.16 ± 0.83, integral of force = 0.12 ± 0.84, and P200m = 0.05 ± 0.30). For 30CJ the results were similar, with improvements in both groups (placebo, Δ = 14.9% ± 14.1%; β-alanine, Δ = 16.9% ± 18.5%) but with no significant interaction effect between groups and an unclear effect (0.14 ± 0.75). Conclusion Four weeks of β-alanine supplementation does not substantially improve performance of 30TS, P200m, or 30CJ in highly trained water polo athletes compared with a control group.

Schedule Analytics

DTIC Science & Technology

2016-04-30

1990’s to Today MAIS MDAP 12.75 Years 7.9 Years 43 Months 68 Months 42 Months A B C IOC 31m 41m 23m FDD A B C A B C Materiel...to FDD 26 2m 9m 16m 41m 72m Months MS A to MS B MS B to MS C MS C to FDD 10 20 30 40 50 60 70 80 90...vs. Schedule 0 10 20 30 40 50 60 70 80 90 100 110 120 >$1B $500M-$1B <$500M M on th s MAIS MS A – MS B MS B – MS C MS C – FDD Total © 2016 The MITRE

Which DEM is the best for glaciology? -Evaluation of global-scale DEM products-

NASA Astrophysics Data System (ADS)

Nagai, Hiroto; Tadono, Takeo

2017-04-01

Digital elevation models (DEMs) are fundamental geospatial data to study glacier distribution, changes, dynamics, mass balance and various geomorphological conditions. This study evaluates latest global-scale free DEMs in order to clarify their superiority and inferiority in glaciological uses. Three DEMs are now available; the 1-arcsec. product obtained from the Shuttle Radar Topographic Mission (SRTM1), the second version of Global Digital Elevation Model of the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER GDEM2), and the first resampled dataset acquired by the Advanced Land observing Satellite, namely ALOS World 3D-30m (AW3D30). These DEMs have common specifications of global coverage (<60°S/N for SRTM1), freely downloadable via internet, and 1-arcsec. ( 30 m) pixel spacing. We carried out quantitative accuracy evaluation and spatial analysis of missing data (i.e. "void") distribution for these DEMs. Elevation values of the three DEMs are validated at check points (CPs), where elevation was measured by Geospatial Information Authority of Japan, in (A) the Japan Alps (as steep mountains with glaciation), in (B) Mt. Fuji (as monotonous hillslope), and in (C) the Tone river basin (as an flat plain). In all study sites, AW3D30 has the smallest errors against the CP elevation values (A: -6.1±8.6 m, B: +0.1±3.9 m, C: +0.1±2.5 m as the mean value and standard deviation of elevation differences). SRTM1 is secondly accurate (A: -17.8±16.3 m, B: +1.3±6.4 m, C: +0.1±3.1 m,), followed by ASTER GDEM2 (A: -13.9±20.8 m, B: -3.9±10.0 m, C: +4.3±3.8 m,). This accuracy differences among the DEMs are greater in steeper terrains (A>B>C). In the Tone river basin, SRTM1 has equivalent accuracy to AW3D30. High resolution (2.5 m) of the original stereo-pair images for AW3D30 (i.e. ALOS PRISM imagery) contributes for the best absolute accuracy. Glaciers on rather flat terrains are usually distributed in higher latitude (e.g. Antarctica and Greenland), where SRTM1 is unable. Glaciers at mid-to-low latitudes glaciers are usually distributed in high and steep mountains, where SRTM1 has lower accuracy than AW3D30. AW3D30 would contributes as a preferable option for glaciology in a global scale. At the tops of high mountains in the Nepal Himalaya, however, AW3D30 has a large area of data missing due to snow cover. This inferiority should be improved by filling with other datasets in the next version. ASTER GDEM2 has less area of data missing in the Nepal Himalaya, which would contribute for coarse uses such as generation of river basin, brief drawing of a topographic map, etc.

Comparison of Efficacy and Safety of Liraglutide 3.0 mg in Individuals with BMI above and below 35 kg/m²: A Post-hoc Analysis

PubMed Central

le Roux, Carel; Aroda, Vanita; Hemmingsson, Joanna; Cancino, Ana Paula; Christensen, Rune; Pi-Sunyer, Xavier

2018-01-01

Objective To investigate whether the efficacy and safety of liraglutide 3.0 mg differed between two subgroups, BMI 27 to <35 and BMI ≥ 35 kg/m², in individuals without and with type 2 diabetes (T2D). Methods A post-hoc analysis of two 56-week, randomized, double-blind, placebo-controlled trials (SCALE Obesity and Prediabetes; SCALE Diabetes). Subgroup differences in treatment effects of liraglutide 3.0 mg were evaluated by testing the interaction between treatment group and baseline BMI subgroup. Results Significantly greater weight loss (0–56 weeks) was observed with liraglutide 3.0 mg versus placebo in all patient groups while on treatment. There was no evidence that the weight-lowering effect of liraglutide 3.0 mg differed between BMI subgroups (interaction p > 0.05). Similarly, for most secondary endpoints significantly greater improvements were observed with liraglutide 3.0 mg versus placebo, with no indication treatment effects differing between subgroups. The safety profile of liraglutide 3.0 mg was broadly similar across BMI subgroups. Conclusion This post-hoc analysis did not indicate any differences in the treatment effects, or safety profile, of liraglutide 3.0 mg for individuals with BMI 27 to <35 or ≥35 kg/m². Liraglutide 3.0 mg can therefore be considered for individuals with a BMI of ≥35 as well as for those with a BMI of 27 to <35 kg/m². PMID:29145215

Evidence for P(2)-purinoceptors contribution in H(2)O(2)-induced contraction of rat aorta in the absence of endothelium.

PubMed

Shen, J Z; Zheng, X F; Kwan, C Y

2000-08-18

H(2)O(2) can contract many arteries, however the underlying mechanisms are not fully understood. This study aims to test whether H(2)O(2)-induced vasoconstriction could be functionally attributed to the activation of P(2)-purinoceptors in rat aorta and to explore its possible signaling mechanisms. Isometric tension recording of H(2)O(2) and ATP-induced contractions of rat aortic rings were compared in the absence or presence of various pharmacological tools to identify their possible common signaling pathways. Both H(2)O(2) and ATP induced transient phasic contractions in a concentration-dependent manner (1-1000 microM). Removal of endothelium potentiated the contractile responses to H(2)O(2) and to ATP. H(2)O(2) (30 microM)-induced phasic contraction could be abolished by catalase (800 U/ml), but not affected by SOD (150 U/ml), DMSO (5 mM) and apyrase (5 U/ml), suggesting no involvement of O(2)(-), hydroxyl free radicals and ATP release. Also, several receptor antagonists including phentolamine, atropine, methysergide and chlorpheniramine (each 3 microM) were without effect on H(2)O(2) (30 microM)-induced phasic contraction, suggesting no involvement of typical neurotransmitter release. However, both H(2)O(2) (30 microM) and ATP (1 mM)-induced phasic contractions not only presented homologous desensitization, but also showed heterogeneous desensitization. Furthermore, the phasic contractions in response to H(2)O(2) (30 microM) or ATP (100 microM) could be inhibited or abolished in a concentration dependent manner by RB-2 and suramin (10-100 microM), two widely used P(2)-purinoceptor antagonists, with only partial inhibition by Evans blue (300 microM), a moderately selective P(2x) receptor blocker, or by alpha-beta-methylene-ATP (100 microM), a selective P(2x) receptor desensitizer. On the other hand, both H(2)O(2) (30 microM) and ATP (100 microM)-induced phasic contractions were also attenuated, to different degree, by inhibitors of several enzymes including PLC, PKC, PLA(2) and cyclooxygenase. Lastly, removal of extracellular Ca(2+) or pretreatment with procaine (10 mM) and dantrolene (30 microM), two putative intracellular Ca(2+) release blockers, or with Ni(2+) (100 microM) and tetrandrine (5 microM), two Ca(2+) channel blockers, all significantly inhibited H(2)O(2) and ATP-induced contractions. However, nifedipine (1 microM), a voltage-dependent L-type Ca(2+) channel blocker, was without effect. Our results demonstrate that H(2)O(2)-induced phasic contraction of rat aorta involves, at least in part, the activation of P(2)-purinoceptors in the aortic smooth muscle cells

Global Web-Enabled Landsat Data (Invited)

NASA Astrophysics Data System (ADS)

Roy, D. P.; Kovalskyy, V.; Kommareddy, I.; Votava, P.; Nemani, R. R.; Egorov, A.; Hansen, M.; Yan, L.

2013-12-01

The 40+ year series of Landsat satellites provides the longest temporal record of space-based observations acquired with spatial resolutions appropriate for monitoring anthropogenic change. The need for 'higher-level' Landsat products, i.e., beyond currently available radiometrically and geometrically corrected Landsat scenes, has been advocated by the user community and by the Landsat science team. The NASA funded Web-enabled Landsat Data (WELD) project has demonstrated this capability by systematically generating 30m weekly, seasonal, monthly and annual composited Landsat mosaics of the conterminous United States (CONUS) and Alaska for 10+ years (http://weld.cr.usgs.gov/). Recently, the WELD code has been ported to the NASA Earth Exchange (NEX) high performance super computing and data platform to generate global 30m WELD products from contemporaneous Landsat 5 and 7 data. The WELD products and select applications that take advantage of the consistently processed WELD time series are showcased. Prototype global monthly 30m products and plans to expand the production to provide Landsat 30m higher level products for any terrestrial non-Antarctic location for six 3-year epochs from 1985 to 2010 are presented. Prototype monthly global NEX 30m WELD product

How do you manage early or late clock-ins? .

PubMed

Marlowe, Cathy L

2013-08-01

My current practice has historically been very strict with hourly employees. The workday is 8:30 a.m. to 5 p.m. for most employees. They are to clock in and out at exactly those times unless overtime has been approved. (Staff working with late patients are exempt.) My business manager is constantly fighting over clock-ins 15 to 20 minutes early or late, with similar clock-outs. Staff may have a stop to make and would like to make up those 15 minutes. We do grant paid time off in an initial minimum block of 30 minutes to assist with some of this. ... The issue is not overtime, as we're well aware that we have to pay for it when appropriate. It's simply to streamline our policy and make it fair. ... I'm looking for practice policies that deal with employee variances from the 8 a.m. to 4:30 p.m. clock-ins. For example, someone wants to come in 30 minutes early in order to leave 30 minutes early for an appointment. Or someone comes in 22 minutes late due to traffic and wants either to make it up or to take the minimal half-hour paid-time-off time.

Generation of the 30 M-Mesh Global Digital Surface Model by Alos Prism

NASA Astrophysics Data System (ADS)

Tadono, T.; Nagai, H.; Ishida, H.; Oda, F.; Naito, S.; Minakawa, K.; Iwamoto, H.

2016-06-01

Topographical information is fundamental to many geo-spatial related information and applications on Earth. Remote sensing satellites have the advantage in such fields because they are capable of global observation and repeatedly. Several satellite-based digital elevation datasets were provided to examine global terrains with medium resolutions e.g. the Shuttle Radar Topography Mission (SRTM), the global digital elevation model by the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER GDEM). A new global digital surface model (DSM) dataset using the archived data of the Panchromatic Remote-sensing Instrument for Stereo Mapping (PRISM) onboard the Advanced Land Observing Satellite (ALOS, nicknamed "Daichi") has been completed on March 2016 by Japan Aerospace Exploration Agency (JAXA) collaborating with NTT DATA Corp. and Remote Sensing Technology Center, Japan. This project is called "ALOS World 3D" (AW3D), and its dataset consists of the global DSM dataset with 0.15 arcsec. pixel spacing (approx. 5 m mesh) and ortho-rectified PRISM image with 2.5 m resolution. JAXA is also processing the global DSM with 1 arcsec. spacing (approx. 30 m mesh) based on the AW3D DSM dataset, and partially releasing it free of charge, which calls "ALOS World 3D 30 m mesh" (AW3D30). The global AW3D30 dataset will be released on May 2016. This paper describes the processing status, a preliminary validation result of the AW3D30 DSM dataset, and its public release status. As a summary of the preliminary validation of AW3D30 DSM, 4.40 m (RMSE) of the height accuracy of the dataset was confirmed using 5,121 independent check points distributed in the world.

Maternal obesity is the new challenge; a qualitative study of health professionals' views towards suitable care for pregnant women with a Body Mass Index (BMI) ≥ 30 kg/m².

PubMed

Smith, Debbie M; Cooke, Alison; Lavender, Tina

2012-12-19

An increase in the number of women with maternal obesity (Body Mass Index [BMI] ≥30 kg/m2) has had a huge impact on the delivery of maternity services. As part of a programme of feasibility work to design an antenatal lifestyle programme for women with a BMI ≥30 kg/m2, the current study explored health professionals' experiences of caring for women with a BMI ≥30 kg/m2 and their views of the proposed lifestyle programme. Semi-structured interviews with 30 health professionals (including midwives, sonographers, anaesthetists and obstetricians) were conducted and analysed using thematic analysis. Recruitment occurred in two areas in the North West of England in early 2011. Three themes were evident. Firstly, obesity was seen as a conversation stopper; obesity can be a challenge to discuss. Secondly, obesity was seen as a maternity issue; obesity has a direct impact on maternity care and therefore intervention is needed. Finally, the long-term impact of maternal obesity intervention; lifestyle advice in pregnancy has the potential to break the cyclic obesity relationship. The health professionals believed that antenatal lifestyle advice can play a key role in addressing the public health issue of obesity as pregnancy is a time of increased motivation for women with a BMI ≥30 kg/m2. Maternal obesity is a challenge and details of the training content required for health professionals to feel confident to approach the issue of maternal obesity with women are presented. Support for the antenatal lifestyle programme for women with a BMI ≥30 kg/m2 highlights the need for further exploration of the impact of interventions on health promotion.

Houston-Galveston Navigation Channels Texas Project. Report 1. Galveston Bay Field Investigation

DTIC Science & Technology

1994-07-01

Botim (Continued) 1810 14.8 2.8 314 1909 14.6 3.2 312 2018 14.0 3.3 308 2109 14.0 3.1 310 2210 14.0 2.3 319 2311 13.8 0.6 324 0011 14.3 0.8 139 0110...0.6 360 1216 3.0 0.4 345 1321 3.0 0.3 318 1412 3.0 0.4 345 1520 3.0 0.7 12 1619 3.0 1.0 10 1723 3.0 1.4 360 1615 3.0 1.5 354 1924 3.0 1.5 350 2018 3.0...nhV jispunded S~d~mM csar n_____ _____ 1811 10.2 24.25 30 1915 10.2 21.76 30 2018 10.5 20.07 26 2115 10.2 19.23 40 2214 10.0 18.22 10 2313 10.5 19.74

76 FR 7230 - Notice of Public Meeting, Coeur d'Alene District Resource Advisory Council Meeting; Idaho

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-09

... Advisory Council (RAC) will meet as indicated below. DATES: March 22, 2011. The meeting will start at 8 a.m. and end at about 3:30 p.m. with the public comment period from 1 p.m. to 1:30 p.m. The meeting will be... FURTHER INFORMATION CONTACT: Lisa Wagner, RAC Coordinator, BLM Coeur d'Alene District, 3815 Schreiber Way...

76 FR 15302 - Notice of Public Hearing for the Draft Environmental Impact Statement for the TRIDENT Support...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-21

.... April 21, 2011, at the Seattle Central Library, 1000 Fourth Avenue, Seattle, WA 98104. All meetings will start with an open house session from 6 p.m. to 7:30 p.m. followed by a presentation and public comment period from 7:30 p.m. to 9 p.m. The open house sessions will allow individuals the opportunity to review...

75 FR 8062 - Science Advisory Board Staff Office; Notification of a Public Meeting and Public Teleconference(s...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-23

... (January 2010) and Quantitative Health Risk Assessment for Particulate Matter--Second External Review Draft... teleconference will be held on April 9, 2010 in the event more time is needed to discuss EPA's Policy Assessment...:30 a.m. to 5 p.m. (Eastern Time) and March 11, 2010 from 8:30 a.m. to 2 p.m. (Eastern Time). A public...

77 FR 74817 - Approval and Promulgation of Air Quality Implementation Plans; Wisconsin; Disapproval of PM2.5

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-18

... hours of business are Monday through Friday, 8:30 a.m. to 4:30 p.m., excluding Federal holidays... to be Confidential Business Information (CBI) or other information whose disclosure is restricted by...) and (4m); (6) amend NR 405.02(25k)(intro.); (7) create NR 405.02(27)(a)5m in Table A; (8) amend NR 405...

Microscale 3D Liver Bioreactor for In Vitro Hepatotoxicity Testing under Perfusion Conditions.

PubMed

Freyer, Nora; Greuel, Selina; Knöspel, Fanny; Gerstmann, Florian; Storch, Lisa; Damm, Georg; Seehofer, Daniel; Foster Harris, Jennifer; Iyer, Rashi; Schubert, Frank; Zeilinger, Katrin

2018-03-15

The accurate prediction of hepatotoxicity demands validated human in vitro models that can close the gap between preclinical animal studies and clinical trials. In this study we investigated the response of primary human liver cells to toxic drug exposure in a perfused microscale 3D liver bioreactor. The cellularized bioreactors were treated with 5, 10, or 30 mM acetaminophen (APAP) used as a reference substance. Lactate production significantly decreased upon treatment with 30 mM APAP ( p < 0.05) and ammonia release significantly increased in bioreactors treated with 10 or 30 mM APAP ( p < 0.0001), indicating APAP-induced dose-dependent toxicity. The release of prostaglandin E2 showed a significant increase at 30 mM APAP ( p < 0.05), suggesting an inflammatory reaction towards enhanced cellular stress. The expression of genes involved in drug metabolism, antioxidant reactions, urea synthesis, and apoptosis was differentially influenced by APAP exposure. Histological examinations revealed that primary human liver cells in untreated control bioreactors were reorganized in tissue-like cell aggregates. These aggregates were partly disintegrated upon APAP treatment, lacking expression of hepatocyte-specific proteins and transporters. In conclusion, our results validate the suitability of the microscale 3D liver bioreactor to detect hepatotoxic effects of drugs in vitro under perfusion conditions.

Microscale 3D Liver Bioreactor for In Vitro Hepatotoxicity Testing under Perfusion Conditions

PubMed Central

Freyer, Nora; Greuel, Selina; Knöspel, Fanny; Gerstmann, Florian; Storch, Lisa; Damm, Georg; Seehofer, Daniel; Foster Harris, Jennifer; Iyer, Rashi; Schubert, Frank; Zeilinger, Katrin

2018-01-01

The accurate prediction of hepatotoxicity demands validated human in vitro models that can close the gap between preclinical animal studies and clinical trials. In this study we investigated the response of primary human liver cells to toxic drug exposure in a perfused microscale 3D liver bioreactor. The cellularized bioreactors were treated with 5, 10, or 30 mM acetaminophen (APAP) used as a reference substance. Lactate production significantly decreased upon treatment with 30 mM APAP (p < 0.05) and ammonia release significantly increased in bioreactors treated with 10 or 30 mM APAP (p < 0.0001), indicating APAP-induced dose-dependent toxicity. The release of prostaglandin E2 showed a significant increase at 30 mM APAP (p < 0.05), suggesting an inflammatory reaction towards enhanced cellular stress. The expression of genes involved in drug metabolism, antioxidant reactions, urea synthesis, and apoptosis was differentially influenced by APAP exposure. Histological examinations revealed that primary human liver cells in untreated control bioreactors were reorganized in tissue-like cell aggregates. These aggregates were partly disintegrated upon APAP treatment, lacking expression of hepatocyte-specific proteins and transporters. In conclusion, our results validate the suitability of the microscale 3D liver bioreactor to detect hepatotoxic effects of drugs in vitro under perfusion conditions. PMID:29543727

Rapid presumptive identification of the Mycobacterium tuberculosis-bovis complex by radiometric determination of heat stable urease

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gandy, J.H.; Pruden, E.L.; Cox, F.R.

1983-12-01

Simple and rapid Bactec methodologies for the determination of neat (unaltered) and heat stable urease activity of mycobacteria are presented. Clinical isolates (63) and stock cultures (32)--consisting of: M. tuberculosis (19), M. bovis (5), M. kansasii (15), M. marinum (4), M. simiae (3), M. scrofulaceum (16), M. gordonae (6), M. szulgai (6), M. flavescens (1), M. gastri (1), M. intracellulare (6), M. fortuitum-chelonei complex (12), and M. smegmatis (1)--were tested for neat urease activity by Bactec radiometry. Mycobacterial isolates (50-100 mg wet weight) were incubated at 35 degrees C for 30 minutes with microCi14C-urea. Urease-positive mycobacteria gave Bactec growth indexmore » (GI) values greater than 100 units, whereas urease-negative species gave values less than 10 GI units. Eighty-three isolates possessing neat urease activity were heated at 80 degrees C for 30 minutes followed by incubation at 35 degrees C for 30 minutes with 1 microCi14C-urea. Mycobacterium tuberculosis-bovis complex demonstrated heat-stable urease activity (GI more than 130 units) and could be distinguished from mycobacteria other than tuberculosis (MOTT), which gave GI values equal to or less than 40 units.« less

Modeling and Simulation of Ceramic Arrays to Improve Ballaistic Performance

DTIC Science & Technology

2013-07-01

Ref: ARL-TR- 2219 , 2000.) Al 5083 .30 Caliber AP-M2 E ^Projectile — 3.918 mm tAj = 76.2 mm H = 20.0 mm VP = 400 - 900 m/s Al ^ Al H 2013...reference - ARL-TR- 2219 , 2000. 15. SUBJECT TERMS .30cal AP M2 Projectile, 762x39 PS Projectile, SPH, Aluminum 5083, SiC, DoP Expeminets, AutoDyn...on the DoP experiments described in reference - ARL-TR- 2219 , 2000. 2013 © University of Delaware DOP OF .30cal PROJECTILE INTO MONOLITHIC ALUMINUM

New Standards for Diagnosing Hypertension Are Met with Skepticism | Poster

Cancer.gov

Check your blood pressure for free at Occupational Health Services every Tuesday and Thursday as part of Blood Pressure Awareness Month. Free screening is available every Tuesday during the Farmers’ Market in Building 549 from 11:30 a.m. to 1:30 p.m, and every Tuesday and Thursday in Building 426 from 12:30 to 1 p.m. Sarah Hooper, manager of Occupational Health Services (OHS), said the health of the employees at NCI at Frederick is important to OHS, and OHS staff wants the community to stay informed.

2007 U.S. Coast Guard Innovation Expo Volume 1

DTIC Science & Technology

2007-11-01

Orleans Ballroom Foyer 3:00 p.m. - 7:30 p.m. Expo Floor Opens Exhibit Hall E; 1st level 6:00 p.m. - 7:30 p.m. NDIA Welcome Reception ... Reception “Bamboula” Spanish Plaza/Riverwalk (shuttle bus service from convention center to Spanish Plaza) • • • • 7:00 a.m. - 5...physiological cues of “hostile intent” Double or triple wide trailer tested at various sites around the country Resilient Electric Grid – System that

High soluble CD30 levels and associated anti-HLA antibodies in patients with failed renal allografts.

PubMed

Karahan, Gonca E; Caliskan, Yasar; Ozdilli, Kursat; Kekik, Cigdem; Bakkaloglu, Huseyin; Caliskan, Bahar; Turkmen, Aydin; Sever, Mehmet S; Oguz, Fatma S

2017-01-13

Serum soluble CD30 (sCD30), a 120-kD glycoprotein that belongs to the tumor necrosis factor receptor family, has been suggested as a marker of rejection in kidney transplant patients. The aim of this study was to evaluate the relationship between sCD30 levels and anti-HLA antibodies, and to compare sCD30 levels in patients undergoing hemodialysis (HD) with and without failed renal allografts and transplant recipients with functioning grafts. 100 patients undergoing HD with failed grafts (group 1), 100 patients undergoing HD who had never undergone transplantation (group 2), and 100 kidney transplant recipients (group 3) were included in this study. Associations of serum sCD30 levels and anti-HLA antibody status were analyzed in these groups. The sCD30 levels of group 1 and group 2 (154 ± 71 U/mL and 103 ± 55 U/mL, respectively) were significantly higher than those of the transplant recipients (group 3) (39 ± 21 U/mL) (p<0.001 and p<0.001). The serum sCD30 levels in group 1 (154 ± 71 U/mL) were also significantly higher than group 2 (103 ± 55 U/mL) (p<0.001). Anti-HLA antibodies were detected in 81 (81%) and 5 (5%) of patients in groups 1 and 2, respectively (p<0.001). When multiple regression analysis was performed to predict sCD30 levels, the independent variables in group 1 were the presence of class I anti-HLA antibodies (β = 0.295; p = 0.003) and age (β = -0.272; p = 0.005), and serum creatinine (β = 0.218; p = 0.027) and presence of class II anti-HLA antibodies (standardized β = 0.194; p = 0.046) in group 3. Higher sCD30 levels and anti-HLA antibodies in patients undergoing HD with failed renal allografts may be related to higher inflammatory status in these patients.

Evaluation of Antimicrobial Activities of Sequential Spray Applications of Decontamination Treatments on Chicken Carcasses

PubMed Central

Benli, Hakan; Sanchez-Plata, Marcos X.; Ilhak, Osman Irfan; Núñez De González, Maryuri T.; Keeton, Jimmy T.

2015-01-01

The objective of this study was to evaluate the effects of sequential applications of ɛ-polylysine (EPL) or lauramide arginine ethyl ester (LAE) sprays followed by an acidic calcium sulfate (ACS) spray on inoculated chicken carcasses to reduce Salmonella (Salmonella enterica serovars including Salmonella typhimurium and Salmonella enteritidis) contamination during 6 days of storage (4.4°C). Secondly, reductions of the resident microflora were studied on uninoculated chicken carcasses following the sequential application of the treatments, chilling and 10 days of storage at 4.4°C. The treatment of Salmonella inoculated carcasses with 300 mg/L EPL followed by 30% ACS (EPL300-ACS30) sprays reduced Salmonella counts initially by 1.5 log cfu/mL and then by 1.2 log cfu/mL (p<0.05) following 6 days of storage at 4.4°C. Likewise, 200 mg/L LAE followed by 30% ACS (LAE200-ACS30) treatment reduced initial Salmonella counts on poultry carcasses by 1.8, 1.4 and 1.8 log cfu/mL (p<0.05), respectively, after 0, 3, and 6 days storage. Immediately after the treatments, EPL300-ACS30 and LAE200-ACS30 both reduced Escherichia coli counts significantly by 2.6 and 2.9 log cfu/mL, respectively. EPL300-ACS30 and LAE200-ASC30 were effective in lowering psychrotroph counts by 1 log cfu/mL on day 10 when compared to the control and distilled water treatments. This study demonstrated that EPL300-ACS30 and LAE200-ACS30 were effective in reducing Salmonella on inoculated chicken carcasses both after treatment and during the storage at 4.4°C for up to 6 days. In addition, reductions in psychrotroph counts indicated that these treatments might have the potential to increase the shelf-life of poultry carcasses. PMID:25656180

Additive Neuroprotective Effects of the Multifunctional Iron Chelator M30 with Enriched Diet in a Mouse Model of Amyotrophic Lateral Sclerosis.

PubMed

Golko-Perez, Sagit; Mandel, Silvia; Amit, Tamar; Kupershmidt, Lana; Youdim, Moussa B H; Weinreb, Orly

2016-02-01

Amyotrophic lateral sclerosis (ALS) is the most common degenerative disease of the motoneuron system, involving various abnormalities, such as mitochondrial dysfunction, oxidative stress, transitional metal accumulation, neuroinflammation, glutamate excitotoxicity, apoptosis, decreased supply of trophic factors, cytoskeletal abnormalities, and extracellular superoxide dismutase (SOD)-1 toxicity. These multiple disease etiologies implicated in ALS gave rise to the perception that future therapeutic approaches for the disease should be aimed at targeting multiple pathological pathways. In line with this view, we have evaluated in the current study the therapeutic effects of low doses of the novel multifunctional monoamine oxidase (MAO) inhibitor/iron-chelating compound, M30 in combination with high Calorie Energy supplemented Diet (CED) in the SOD1-G93A transgenic mouse model of ALS. Our results demonstrated that the combined administration of M30 with CED produced additive neuroprotective effects on motor performance and increased survival of SOD1-G93A mice. We also found that both M30 and M30/CED regimens caused a significant inhibition of MAO-A and -B activities and decreased the turnover of dopamine in the brain of SOD1-G93A mice. In addition, M30/CED combined treatment resulted in a significant increase in mRNA expression levels of various mitochondrial biogenesis and metabolism regulators, such as peroxisome proliferator-activated receptor-γ (PPARγ)-co activator 1 alpha (PGC-1α), PPARγ, uncoupling protein 1, and insulin receptor in the gastrocnemius muscle of SOD1-G93A mice. These results suggest that a combination of drug/agents with different, but complementary mechanisms may be beneficial in the treatment of ALS.

Variation in rod and cone density from the fovea to the mid-periphery in healthy human retinas using adaptive optics scanning laser ophthalmoscopy

PubMed Central

Wells-Gray, E M; Choi, S S; Bries, A; Doble, N

2016-01-01

Purpose To characterize the rod and cone photoreceptor mosaic at retinal locations spanning the central 60° in vivo using adaptive optics scanning laser ophthalmoscopy (AO-SLO) in healthy human eyes. Methods AO-SLO images (0.7 × 0.9°) were acquired at 680 nm from 14 locations from 30° nasal retina (NR) to 30° temporal retina (TR) in 5 subjects. Registered averaged images were used to measure rod and cone density and spacing within 60 × 60 μm regions of interest. Voronoi analysis was performed to examine packing geometry at all locations. Results Average peak cone density near the fovea was 164 000±24 000 cones/mm2 and decreased to 6700±1500 and 5400±700 cones/mm2 at 30° NR and 30° TR, respectively. Cone-to-cone spacing increased from 2.7±0.2 μm at the fovea to 14.6±1.4 μm at 30° NR and 16.3±0.7 μm at 30° TR. Rod density peaked at 25° NR (124 000±20 000 rods/mm2) and 20° TR (120 000±12 000 rods/mm2) and decreased at higher eccentricities. Center-to-center rod spacing was lowest nasally at 25° (2.1±0.1 μm). Temporally, rod spacing was lowest at 20° (2.2±0.1 μm) before increasing to 2.3±0.1 μm at 30° TR. Conclusions Both rod and cone densities showed good agreement with histology and prior AO-SLO studies. The results demonstrate the ability to image at higher retinal eccentricities than reported previously. This has clinical importance in diseases that initially affect the peripheral retina such as retinitis pigmentosa. PMID:27229708

77 FR 15846 - Advisory Committee on Minority Veterans; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-16

... 24, from 9 a.m. to 3:30 p.m., Fort Sam Houston National Cemetery, 1520 Harry Wurzbach Road, San Antonio, TX; and VA South Bexar County Community Outpatient Center, 4610 South Cross, San Antonio, TX...:30 p.m.; VA Health Care Center at Harlingen, 2701 South 77 Sunshine Strip, Harlingen, TX; and...

75 FR 79006 - Council on Graduate Medical Education; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-17

... Graduate Medical Education; Notice of Meeting In accordance with section 10(a)(2) of the Federal Advisory... Medical Education (COGME). Dates and Times: January 19, 2011, 8:30 a.m.-4 p.m., January 20, 2011, 8:30 a.m... first day will consist of presentations covering various aspects of graduate medical education, Bureau...

76 FR 12136 - Advisory Committee for Geosciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-04

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Geosciences; Notice of Meeting In accordance with the Federal Advisory Committee Act (Pub. L. 92- 463, as amended), the National Science Foundation announces the following meeting: Name: Advisory Committee for Geosciences (1755). Dates: April 13, 2011; 8:30 a.m.- 5 p.m., April 14, 2011; 8:30 a.m...

77 FR 22823 - Sunshine Act Meetings; Board of Governors

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-17

... POSTAL SERVICE Sunshine Act Meetings; Board of Governors DATES AND TIMES: Thursday, May 3, 2012, at 10 a.m.; and Friday, May 4, at 8:30 a.m. and 10:30 a.m. PLACE: Washington, DC, at U.S. Postal Service Headquarters, 475 L'Enfant Plaza SW., in the Benjamin Franklin Room. STATUS: Thursday, May 3 at 10...

78 FR 5213 - Board of Governors; Sunshine Act Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-24

... POSTAL SERVICE Board of Governors; Sunshine Act Meeting DATES AND TIMES: Thursday, February 7, 2013, at 10:00 a.m.; and Friday, February 8, at 8:30 a.m. and 10:30 a.m. PLACE: Washington, DC, at U.S. Postal Service Headquarters, 475 L'Enfant Plaza SW., in the Benjamin Franklin Room. STATUS: Thursday...

77 FR 43128 - Board of Governors Sunshine Act Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-23

... POSTAL SERVICE Board of Governors Sunshine Act Meeting DATES AND TIMES: Wednesday, August 8, 2012, at 10:00 a.m.; Thursday, August 9, at 8:30 a.m. and 10:30 a.m. PLACE: Washington, DC, at U.S. Postal Service Headquarters, 475 L'Enfant Plaza SW., in the Benjamin Franklin Room. STATUS: Wednesday, August 8...

76 FR 43737 - Board of Governors; Sunshine Act Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-21

... POSTAL SERVICE Board of Governors; Sunshine Act Meeting DATES AND TIMES: Thursday, August 4, 2011, at 10 a.m.; and Friday, August 5, at 8:30 a.m. and 10:30 a.m. PLACE: Washington, DC, at U.S. Postal Service Headquarters, 475 L'Enfant Plaza, S.W., in the Benjamin Franklin Room. STATUS: Thursday, August 4...

Relationships and Predictive Capabilities of Jump Assessments to Soccer-Specific Field Test Performance in Division I Collegiate Players.

PubMed

Lockie, Robert G; Stage, Alyssa A; Stokes, John J; Orjalo, Ashley J; Davis, DeShaun L; Giuliano, Dominic V; Moreno, Matthew R; Risso, Fabrice G; Lazar, Adrina; Birmingham-Babauta, Samantha A; Tomita, Tricia M

2016-12-03

Leg power is an important characteristic for soccer, and jump tests can measure this capacity. Limited research has analyzed relationships between jumping and soccer-specific field test performance in collegiate male players. Nineteen Division I players completed tests of: leg power (vertical jump (VJ), standing broad jump (SBJ), left- and right-leg triple hop (TH)); linear (30 m sprint; 0⁻5 m, 5⁻10 m, 0⁻10, 0⁻30 m intervals) and change-of-direction (505) speed; soccer-specific fitness (Yo-Yo Intermittent Recovery Test Level 2); and 7 × 30-m sprints to measure repeated-sprint ability (RSA; total time (TT), performance decrement (PD)). Pearson's correlations ( r ) determined jump and field test relationships; stepwise regression ascertained jump predictors of the tests ( p < 0.05). All jumps correlated with the 0⁻5, 0⁻10, and 0⁻30 m sprint intervals ( r = -0.65⁻-0.90). VJ, SBJ, and left- and right-leg TH correlated with RSA TT ( r = -0.51⁻-0.59). Right-leg TH predicted the 0⁻5 and 0⁻10 m intervals (R² = 0.55⁻0.81); the VJ predicted the 0⁻30 m interval and RSA TT (R² = 0.41⁻0.84). Between-leg TH asymmetry correlated with and predicted left-leg 505 and RSA PD ( r = -0.68⁻0.62; R² = 0.39⁻0.46). Improvements in jumping ability could contribute to faster speed and RSA performance in collegiate soccer players.

The Impact of In Vitro Accelerated Aging, Approximating 30 and 60 Years In Vivo, on Commercially Available Zirconia Dental Implants.

PubMed

Monzavi, Mona; Noumbissi, Sammy; Nowzari, Hessam

2017-04-01

Despite increased popularity of Zirconia dental implants, concerns have been raised regarding low temperature degradation (LTD) and its effect on micro-structural integrity. This study evaluated the effect of LTD on four types of Zirconia dental implants at 0, 30, and 60 years of artificial aging. The impact of aging on t-m transformation and micro crack formation was measured. Accelerated aging at 15 and 30 hours, approximating 30 and 60 years in vivo, aged 36 Zirconia dental implants: Z systems ® (A), Straumann ® (B), Ceraroot ® (C), and Zeramex ® (D). Focused ion beam-scanning electron microscopic analysis determined the micro structural features, phase transformation, and the formation of micro cracks. At 15 hours, type A implant presented with micro cracks and t-m transformation of 0.9 µm and 3.1 µm, respectively. At 30 hours, micro cracks remained shallow (1 µm). At 15 hours, type B implant presented micro cracks (0.7 µm) and grain transformation (1.2 µm). At 30 hours, these features remained superficial at 0.6 and 1.5 µm, respectively. Type C implant presented surface micro cracks of 0.3 µm at 15 hours. The depth of t-m transformation slightly increased to 1.4 µm. At 30 hours, number of micro cracks increased at the surface to an average depth of 1.5 µm. Depth of t-m transformation increased to an average of 2.5 µm. At 15 hours, micro cracks remained superficial (0.8 µm) for type D implant and depth of t-m transformation increased to 2.3 µm. At 30 hours, the depth of micro cracks increased to an average of 1.3 µm followed by increased t-m transformation to a depth of 4.1 µm. Depth of grain transformation remained within 1-4 µm from the surface. The effect of aging was minimal for all Zirconia implants. © 2016 Wiley Periodicals, Inc.

Site Characterization at Napa Strong Motion Sites Using Tomography, MASW, and MALW

NASA Astrophysics Data System (ADS)

Chan, J. H.; Catchings, R.; Goldman, M.; Criley, C.

2015-12-01

The 24 August 2014 Mw 6.0 South Napa earthquake caused $300 million in damage to private and commercial properties. Previous studies indicate areas underlain by deposits with low average shear-wave velocity to 30 m depth (Vs30) can experience extensive structural damage during earthquakes. Thus, Vs30 is considered a predictor of the influence of local geology on strong shaking from earthquakes. The goal of our study was to evaluate Vs30 at six accelerograph stations in the City and County of Napa and in the City of Vallejo. We used active seismic sources and 4.5-Hz sensors recorded on 120 channels to investigate the shallow velocity structure. Geophones and shots were spaced at 3 m along each profile, which ranged in length from 85 to 260 m. We used a 226-kg accelerated weight-drop and a seisgun to generate P and Rayleigh waves for P-wave tomography and MASW, and we used a 3.5-kg sledgehammer and block to generate S and Love waves for S-wave tomography and MALW. One of the six accelerographs was housed inside Napa Fire Station #3, where the local surface geology consists of late Pleistocene to Holocene alluvium and alluvial fan deposits. The average Vs30 determined from MASW (Rayleigh waves) is 312 m/s, and the average Vs30 from MALW (Love waves) is 340 m/s, with an average velocity difference of about 8% between the two methods. These average values are both slightly less than the average Vs30 of 375 m/s determined from S-wave tomography, which suggests the three methods are complimentary and can be useful in evaluating site response. The 0.42g median horizontal peak ground acceleration (PGA) recorded at Napa Fire Station #3 indicates the area experienced strong shaking during the Mw 6.0 South Napa earthquake, consistent with expectations for a site with relatively low average Vs30.

Crystal Initiation Structures in Developing Enamel: Possible Implications for Caries Dissolution of Enamel Crystals

PubMed Central

Robinson, Colin; Connell, Simon D.

2017-01-01

Investigations of developing enamel crystals using Atomic and Chemical Force Microscopy (AFM, CFM) have revealed a subunit structure. Subunits were seen in height images as collinear swellings about 30 nM in diameter on crystal surfaces. In friction mode they were visible as positive regions. These were similar in size (30–50 nM) to collinear spherical structures, presumably mineral matrix complexes, seen in developing enamel using a freeze fracturing/freeze etching procedure. More detailed AFM studies on mature enamel suggested that the 30–50 nM structures were composed of smaller units, ~10–15 nM in diameter. These were clustered in hexagonal or perhaps a spiral arrangement. It was suggested that these could be the imprints of initiation sites for mineral precipitation. The investigation aimed at examining original freeze etched images at high resolution to see if the smaller subunits observed using AFM in mature enamel were also present in developing enamel i.e., before loss of the organic matrix. The method used was freeze etching. Briefly samples of developing rat enamel were rapidly frozen, fractured under vacuum, and ice sublimed from the fractured surface. The fractured surface was shadowed with platinum or gold and the metal replica subjected to high resolution TEM. For AFM studies high-resolution tapping mode imaging of human mature enamel sections was performed in air under ambient conditions at a point midway between the cusp and the cervical margin. Both AFM and freeze etch studies showed structures 30–50 nM in diameter. AFM indicated that these may be clusters of somewhat smaller structures ~10–15 nM maybe hexagonally or spirally arranged. High resolution freeze etching images of very early enamel showed ~30–50 nM spherical structures in a disordered arrangement. No smaller units at 10–15 nM were clearly seen. However, when linear arrangements of 30–50 nM units were visible the picture was more complex but also smaller units including ~10–15 nM units could be observed. Conclusions: Structures ~10–15 nM in diameter were detected in developing enamel. While the appearance was complex, these were most evident when the 30–5 nM structures were in linear arrays. Formation of linear arrays of subunits may be associated with the development of mineral initiation sites and attendant processing of matrix proteins. PMID:28670283

78 FR 26054 - National Institute on Aging; Notice of Closed Meetings

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2013-05-03

... Validation of Novel Targets for Alzheimer's Disease. Date: June 25, 2013. Time: 11:30 a.m. to 5:30 p.m... Committee: National Institute on Aging Special Emphasis Panel; Drug Development for Alzheimer's Disease...

77 FR 41793 - Center for Scientific Review; Notice of Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-16

... and Population Studies: Special Topics. Date: July 30, 2012. Time: 3:30 p.m. to 4 p.m. Agenda: To.... (Catalogue of Federal Domestic Assistance Program Nos. 93.306, Comparative Medicine; 93.333, Clinical...

Clinical consequences of chemotherapy dose reduction in obese patients with stage III colon cancer: A retrospective analysis from the PETACC 3 study.

PubMed

Stocker, Gertraud; Hacker, Ulrich T; Fiteni, Frédéric; John Mahachie, Jestinah; Roth, Arnaud D; Van Cutsem, Eric; Peeters, Marc; Lordick, Florian; Mauer, Murielle

2018-06-12

Dose reduction in obese cancer patients has been replaced by fully weight-based dosing recommendations. No data, however, are available on the effects of dose reduction in obese stage III colon cancer patients undergoing adjuvant chemotherapy. Survival outcomes and toxicity data of obese (body mass index [BMI] ≥30 kg/m 2 ), stage III colon cancer patients treated within the phase III PETACC 3 trial comparing leucovorin, 5-FU (LV5FU2) with LV5FU2 plus irinotecan were analysed retrospectively according to chemotherapy dosing at first infusion (i.e. fully weight-based dosed - versus dose-reduced group). Multivariate analyses on relapse free survival (RFS) and overall survival (OS) were conducted to adjust for baseline prognostic factors using Cox regression model. 13.4% (280 of 2094 patients) had a BMI ≥ 30 kg/m 2 , and 5.3% had both a BMI ≥ 30 kg/m 2 and a body surface area (BSA) ≥2 m 2 . Dose reductions occurred in 16.1% of patients with a BMI ≥ 30 kg/m 2 and 32.4% with BMI ≥ 30 kg/m 2 and BSA ≥ 2 m 2 , respectively. In patients with BMI ≥ 30 kg/m 2 , multivariate analysis demonstrated a trend towards better RFS in the fully dosed compared to the dose-reduced group (Hazard ratio (HR): 0.69, 95% CI: 0.43-1.09; p = 0.11); however, there was no statistically significant difference in OS. In patients with BMI ≥ 30 kg/m 2 and BSA ≥ 2 m 2 , multivariate analysis demonstrated better RFS in fully dosed compared with dose-reduced patients (HR: 0.48, 95% CI: 0.27-0.85; p = 0.01) and a strong trend towards better OS (HR: 0.53, 95% CI: 0.28-1.01; p = 0.052). This group comprised predominantly of men. Data support the recommendation of using fully dosed chemotherapy for the adjuvant treatment in obese patients with colon cancer. Copyright © 2018 Elsevier Ltd. All rights reserved.

A 3.0-kb deletion including an erythroid cell-specific regulatory element in intron 1 of the ABO blood group gene in an individual with the Bm phenotype.

PubMed

Sano, R; Kuboya, E; Nakajima, T; Takahashi, Y; Takahashi, K; Kubo, R; Kominato, Y; Takeshita, H; Yamao, H; Kishida, T; Isa, K; Ogasawara, K; Uchikawa, M

2015-04-01

We developed a sequence-specific primer PCR (SSP-PCR) for detection of a 5.8-kb deletion (B(m) 5.8) involving an erythroid cell-specific regulatory element in intron 1 of the ABO blood group gene. Using this SSP-PCR, we performed genetic analysis of 382 individuals with Bm or ABm. The 5.8-kb deletion was found in 380 individuals, and disruption of the GATA motif in the regulatory element was found in one individual. Furthermore, a novel 3.0-kb deletion involving the element (B(m) 3.0) was demonstrated in the remaining individual. Comparisons of single-nucleotide polymorphisms and microsatellites in intron 1 between B(m) 5.8 and B(m) 3.0 suggested that these deletions occurred independently. © 2014 International Society of Blood Transfusion.

Low 25-Hydroxyvitamin D Concentrations and Risk of Incident Cognitive Impairment in Black and White Older Adults: The Health ABC Study.

PubMed

Kilpatrick, Laurel; Houston, Denise K; Wilson, Valerie K; Lovato, James; Ayonayon, Hilsa N; Cauley, Jane A; Harris, Tamara; Simonsick, Eleanor M; Yaffe, Kristine; Kritchevsky, Stephen B; Sink, Kaycee M

2018-01-01

Using data from the Health, Aging, and Body Composition study, we examined whether low 25-hydroxyvitamin D (25[OH]D) concentrations were associated with prevalent or incident cognitive impairment. Serum 25(OH)D concentrations were measured in 2,786 older adults and categorized as <20 ng/mL, 20 to <30 ng/mL, or ≥30 ng/mL. Cognitive impairment was defined as a score >1.5 standard deviations below race and education specific means on either digit symbol substitution test or modified mini-mental state test. Logistic regression determined the odds of cognitive impairment at baseline and year 5 by 25(OH)D category. 25(OH)D concentrations were <30 ng/mL in 57.3% of whites and 84.6% of blacks. After excluding participants with baseline cognitive impairment (n = 340), 13% of whites and 13% of blacks developed cognitive impairment by year 5. In whites, 25(OH)D concentrations <30 ng/mL were not associated with prevalent or incident cognitive impairment. Black participants with 25(OH)D concentrations <20 ng/mL had a higher odds of prevalent, but not incident cognitive impairment (OR (95% CI): 2.05 (1.08-3.91), p = 0.03) compared to participants with 25(OH)D concentrations ≥30 ng/mL. Low 25(OH)D concentrations were associated with twofold higher odds of prevalent cognitive impairment in blacks.

Block of Inactivation-deficient Na+ Channels by Local Anesthetics in Stably Transfected Mammalian Cells

PubMed Central

Wang, Sho-Ya; Mitchell, Jane; Moczydlowski, Edward; Wang, Ging Kuo

2004-01-01

According to the classic modulated receptor hypothesis, local anesthetics (LAs) such as benzocaine and lidocaine bind preferentially to fast-inactivated Na+ channels with higher affinities. However, an alternative view suggests that activation of Na+ channels plays a crucial role in promoting high-affinity LA binding and that fast inactivation per se is not a prerequisite for LA preferential binding. We investigated the role of activation in LA action in inactivation-deficient rat muscle Na+ channels (rNav1.4-L435W/L437C/A438W) expressed in stably transfected Hek293 cells. The 50% inhibitory concentrations (IC50) for the open-channel block at +30 mV by lidocaine and benzocaine were 20.9 ± 3.3 μM (n = 5) and 81.7 ± 10.6 μM (n = 5), respectively; both were comparable to inactivated-channel affinities. In comparison, IC50 values for resting-channel block at −140 mV were >12-fold higher than those for open-channel block. With 300 μM benzocaine, rapid time-dependent block (τ ≈ 0.8 ms) of inactivation-deficient Na+ currents occurred at +30 mV, but such a rapid time-dependent block was not evident at −30 mV. The peak current at −30 mV, however, was reduced more severely than that at +30 mV. This phenomenon suggested that the LA block of intermediate closed states took place notably when channel activation was slow. Such closed-channel block also readily accounted for the LA-induced hyperpolarizing shift in the conventional steady-state inactivation measurement. Our data together illustrate that the Na+ channel activation pathway, including most, if not all, transient intermediate closed states and the final open state, promotes high-affinity LA binding. PMID:15545401

Clinical Course and Outcomes of Small Supratentorial Intracerebral Hematomas.

PubMed

Behrouz, Réza; Misra, Vivek; Godoy, Daniel A; Topel, Christopher H; Masotti, Luca; Klijn, Catharina J M; Smith, Craig J; Parry-Jones, Adrian R; Slevin, Mark A; Silver, Brian; Willey, Joshua Z; Masjuán Vallejo, Jaime; Nzwalo, Hipólito; Popa-Wagner, Aurel; Malek, Ali R; Hafeez, Shaheryar; Di Napoli, Mario

2017-06-01

Intracerebral hemorrhage (ICH) volume, particularly if ≥30 mL, is a major determinant of poor outcome. We used a multinational ICH data registry to study the characteristics, course, and outcomes of supratentorial hematomas with volumes <30 mL. Basic characteristics, clinical and radiological course, and 30-day outcomes of these patients were recorded. Outcomes were categorized as early neurological deterioration (END), hematoma expansion, Glasgow Outcome Scale (GOS), and in-hospital death. Poor outcome was defined as composite of in-hospital death and severe disability (GOS ≤ 3). Comparison was conducted based on hemorrhage location. Logistic regression using dichotomized outcome scales was applied to determine predictors of poor outcome. Among 375 cases of supratentorial ICH with volumes <30 mL, expansion and END rates were 19.2% and 7.5%, respectively. Hemorrhage growth was independently associated with END (odds ratio: 28.7, 95% confidence interval [CI]: 8.51-96.5; P < .0001). Expansion rates did not differ according to ICH location. Overall, 13.9% (exact binomial 95% CI: 10.5-17.8) died in the hospital and 29.1% (CI: 24.5-34.0) had severe disability at 30 days; there was a cumulative poor outcome rate of 42.9% (CI: 37.9-48.1). Age, admission Glasgow Coma Scale, intraventricular extension, and END were independently associated with poor outcome. There was no difference in poor outcome rates between lobar and deep locations (40.2% versus 43.8%, P = .56). Patients with supratentorial ICH <30 mL have high rates of poor outcome at 30 days, regardless of location. Nearly 1 in 5 hematomas <30 mL expands, leading to END or death. Copyright © 2017 National Stroke Association. Published by Elsevier Inc. All rights reserved.

Intracellular human papillomavirus E6, E7 mRNA quantification predicts CIN 2+ in cervical biopsies better than Papanicolaou screening for women regardless of age.

PubMed

Pierry, Deirdre; Weiss, Gerald; Lack, Benjamin; Chen, Victor; Fusco, Judy

2012-08-01

Cervical cancer screening in women younger than 30 years relies on cervical cytology because of the poor performance of human papillomavirus (HPV) DNA testing in this age group. To determine the performance of in-cell HPV E6, E7 mRNA quantification (HPV OncoTect) for the detection of high-grade cervical intraepithelial neoplasia in women younger than 30 years. We analyzed 3133 cytology specimens from a screening population of women aged 19-75 years investigate HPV OncoTect as a triage/secondary screening test for atypical squamous cells of undetermined significance (ASCUS) and low-grade squamous intraepithelial lesion (LSIL) cytology in women younger than 30 years. Test results were compared to histology in 246 cases. The sensitivity of E6, E7 mRNA was 89% for CIN 2+ and 100% for CIN 3+ lesions in women 30 years and older. In women younger than 30 years, the sensitivity of E6, E7 mRNA for CIN 2+ lesions was 88% for CIN 2+ and 92% for CIN 3+ lesions. Abnormal cytology (≥ASCUS) exhibited a sensitivity of 89% for CIN 2+ and 100% for CIN 3+ in women 30 years and older and 96% sensitivity for CIN 2+ and 93% sensitivity for CIN 3+ in women younger than 30. The specificity of E6, E7 mRNA was >80% for CIN 2+ and CIN 3+ in both groups of women compared to a specificity of abnormal cytology of <10% for CIN 2+ and CIN 3+ in both groups. HPV OncoTect demonstrates a performance that would be effective for ASCUS/LSIL triage in women including those younger than 30 years.

Design and testing of microfabricated surgical tools for large animal probe insertion

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jorgensen, Shelly

Neural probes provide therapeutic stimulation for neuropsychiatric disorders or record neural activity to investigate the workings of the brain. Researchers utilize 6 mm long temporary silicon stiffeners attached with biodissolvable adhesive to insert flexible neural probes into rat brains, but increasing the probe length fivefold makes inserting large animal probes a significant challenge because of an increased potential for buckling. This study compared the insertion success rates of 6 mm and 30 mm long silicon stiffeners that were 80 μm wide and 30 μm thick, and ascertained the material thickness and modulus of elasticity that would provide successful insertion formore » a 30 mm probe. Using a microdrive, stiffeners were inserted into an agarose brain phantom at controlled insertion speeds while being video-recorded. Twenty-five percent of the 30 mm silicon stiffeners fully inserted at speeds approximately four times higher than the target rate of 0.13 mm/s, while 100 percent of the 6 mm silicon stiffeners inserted successfully at target speed. Critical buckling loads (P cr) were calculated for the 6 mm and 30 mm silicon stiffeners, and for 30 mm diamond and tungsten stiffeners, with thicknesses varying from 30-80 μm. Increasing the thickness of the material by 10 μm, 20 μm and 30 μm improved the P cr by 2.4, 4.7 and 8.2 times, respectively, independent of the material, and substituting diamond for silicon multiplied the buckling capacity by 5.0 times. Stiffeners made of silicon for large animal probe insertion are not strong enough to withstand buckling upon insertion without a significant increase in thickness. Replacing silicon with diamond and increasing the thickness of the stiffener to 50 μm would afford a stiffener with the same P cr capacity as the 6 mm silicon stiffener that had a 100 percent insertion success rate. Experiments should continue with diamond to determine a minimum thickness that will ensure successful insertions and provide an adequate margin of safety.« less

75 FR 55677 - Notice of Enforcement for Special Local Regulation; Thunderboat Regatta; Mission Bay, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-14

... of the Port. DATES: The regulations in 33 CFR 100.1101 will be enforced from 7 a.m. to 5:30 p.m. on... Guard will enforce the special local regulation for the Thunderboat Regatta in 33 CFR 100.1101 on... September 19, 2010, from 7 a.m. PST to 5:30 p.m. Under the provisions of 33 CFR 100.1101, a vessel may not...

Effects of sampling interval on spatial patterns and statistics of watershed nitrogen concentration

USGS Publications Warehouse

Wu, S.-S.D.; Usery, E.L.; Finn, M.P.; Bosch, D.D.

2009-01-01

This study investigates how spatial patterns and statistics of a 30 m resolution, model-simulated, watershed nitrogen concentration surface change with sampling intervals from 30 m to 600 m for every 30 m increase for the Little River Watershed (Georgia, USA). The results indicate that the mean, standard deviation, and variogram sills do not have consistent trends with increasing sampling intervals, whereas the variogram ranges remain constant. A sampling interval smaller than or equal to 90 m is necessary to build a representative variogram. The interpolation accuracy, clustering level, and total hot spot areas show decreasing trends approximating a logarithmic function. The trends correspond to the nitrogen variogram and start to level at a sampling interval of 360 m, which is therefore regarded as a critical spatial scale of the Little River Watershed. Copyright ?? 2009 by Bellwether Publishing, Ltd. All right reserved.

Enhanced biocompatibility and osteogenic potential of mesoporous magnesium silicate/polycaprolactone/wheat protein composite scaffolds

PubMed Central

Kang, Yun Gyeong; Wei, Jie; Shin, Ji Won; Wu, Yan Ru; Su, Jiacan; Park, Young Shik; Shin, Jung-Woog

2018-01-01

Background Successful bone tissue engineering using scaffolds is primarily dependent on the properties of the scaffold, including biocompatibility, highly interconnected porosity, and mechanical integrity. Methods In this study, we propose new composite scaffolds consisting of mesoporous magnesium silicate (m_MS), polycaprolactone (PCL), and wheat protein (WP) manufactured by a rapid prototyping technique to provide a micro/macro porous structure. Experimental groups were set based on the component ratio: (1) WP0% (m_MS:PCL:WP =30:70:0 weight per weight; w/w); (2) WP15% (m_MS:PCL:WP =30:55:15 w/w); (3) WP30% (m_MS:PCL:WP =30:40:30 w/w). Results Evaluation of the properties of fabricated scaffolds indicated that increasing the amount of WP improved the surface hydrophilicity and biodegradability of m_MS/PCL/WP composites, while reducing the mechanical strength. Moreover, experiments were performed to confirm the biocompatibility and osteogenic differentiation of human mesenchymal stem cells (MSCs) according to the component ratio of the scaffold. The results confirmed that the content of WP affects proliferation and osteogenic differentiation of MSCs. Based on the last day of the experiment, ie, the 14th day, the proliferation based on the amount of DNA was the best in the WP30% group, but all of the markers measured by PCR were the most expressed in the WP15% group. Conclusion These results suggest that the m_MS/PCL/WP composite is a promising candidate for use as a scaffold in cell-based bone regeneration. PMID:29520139

Carbon and Nitrogen Mineralization in Relation to Soil Particle-Size Fractions after 32 Years of Chemical and Manure Application in a Continuous Maize Cropping System

PubMed Central

Shao, Xingfang; Zhu, Ping; Zhang, Wenju; Xu, Minggang; Murphy, Daniel V.

2016-01-01

Long-term manure application is recognized as an efficient management practice to enhance soil organic carbon (SOC) accumulation and nitrogen (N) mineralization capacity. A field study was established in 1979 to understand the impact of long-term manure and/or chemical fertilizer application on soil fertility in a continuous maize cropping system. Soil samples were collected from field plots in 2012 from 9 fertilization treatments (M0CK, M0N, M0NPK, M30CK, M30N, M30NPK, M60CK, M60N, and M60NPK) where M0, M30, and M60 refer to manure applied at rates of 0, 30, and 60 t ha−1 yr−1, respectively; CK indicates no fertilizer; N and NPK refer to chemical fertilizer in the forms of either N or N plus phosphorus (P) and potassium (K). Soils were separated into three particle-size fractions (2000–250, 250–53, and <53 μm) by dry- and wet-sieving. A laboratory incubation study of these separated particle-size fractions was used to evaluate the effect of long-term manure, in combination with/without chemical fertilization application, on the accumulation and mineralization of SOC and total N in each fraction. Results showed that long-term manure application significantly increased SOC and total N content and enhanced C and N mineralization in the three particle-size fractions. The content of SOC and total N followed the order 2000–250 μm > 250–53μm > 53 μm fraction, whereas the amount of C and N mineralization followed the reverse order. In the <53 μm fraction, the M60NPK treatment significantly increased the amount of C and N mineralized (7.0 and 10.1 times, respectively) compared to the M0CK treatment. Long-term manure application, especially when combined with chemical fertilizers, resulted in increased soil microbial biomass C and N, and a decreased microbial metabolic quotient. Consequently, long-term manure fertilization was beneficial to both soil C and N turnover and microbial activity, and had significant effect on the microbial metabolic quotient. PMID:27031697

MiR-30c: a novel regulator of salt tolerance in tilapia.

PubMed

Yan, Biao; Guo, Jin-Tao; Zhao, Li-Hui; Zhao, Jin-Liang

2012-08-24

miRNAs comprise a class of ~22 nt noncoding RNAs that modulate the stability and/or translational potential of their mRNA targets. Emerging data suggest that stress conditions can alter the biogenesis of miRNAs, thereby changing the expression of mRNA targets. Here, we reveal that miR-30c, a kidney-enriched miRNA, emerges as a crucial osmoregulator in Nile tilapia. miR-30c loss of function leads to an inability to respond to osmotic stress. We identify HSP70 as one of the direct regulatory targets of miR-30c. miR-30c directly regulates HSP70 by targeting its 3'-UTR, and inhibition of miR-30c substantially increases HSP70 mRNA level in vivo. Taken together, our experiments suggest that miRNAs participate in a regulatory circuit that allows rapid gene program transitions in response to osmotic stress. miR-30c may be developed as a molecular marker to assist to breed or genetically engineer salt tolerant species. Copyright © 2012 Elsevier Inc. All rights reserved.

77 FR 19179 - Emerging Technology and Research Advisory Committee; Notice of Partially Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-30

...: 8:30 a.m.-3:30 p.m. 1. ETRAC Committee Business. 2. Nanotechnology--Nanocoated Materials. 3. Science and Engineering Indicators. 4. ETRAC Committee Discussion. 5. Planning for Next Meeting. The open...

75 FR 57042 - Center for Scientific Review; Notice of Closed Meetings

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2010-09-17

...: Center for Scientific Review Special Emphasis Panel; PAR-08-062: Alzheimer's Disease Pilot Clinical... Diseases. Date: October 25, 2010. Time: 4:30 p.m. to 5:30 p.m. Agenda: To review and evaluate grant...

78 FR 17937 - National Institute of Biomedical Imaging and Bioengineering; Meeting

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2013-03-25

... F. Bolger Center, 9600 Newbridge Drive, Potomac, MD 20854. Closed: 12:30 p.m. to 3:30 p.m. Agenda... Newbridge Drive, Potomac, MD 20854. Contact Person: Anthony Demsey, Ph.D., Director, National Institute of...

75 FR 33629 - Center for Scientific Review; Notice of Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-14

...: Cancer Molecular Therapy. Date: June 17, 2010. Time: 1:30 p.m. to 3:30 p.m. Agenda: To review and... Federal Domestic Assistance Program Nos. 93.306, Comparative Medicine; 93.333, Clinical Research, 93.306...

Rapamycin inhibits oxidative/nitrosative stress and enhances angiogenesis in high glucose-treated human umbilical vein endothelial cells: Role of autophagy.

PubMed

Rezabakhsh, Aysa; Ahmadi, Mahdi; Khaksar, Majid; Montaseri, Azadeh; Malekinejad, Hassan; Rahbarghazi, Reza; Garjani, Alireza

2017-09-01

Chronic hyperglycemia is a potent risk factor of abnormal angiogenesis with various tissue diseases. Autophagy, as an alternative cell response, is mostly generated by a vast array of insults. Applying autophagic response contributes to normal cell retrieval circumstance during various insults. We aimed to show whether stimulation/inhibition of autophagy could reduce or exacerbate oxidative status and angiogenic potential in endothelial cells after exposure to 30mM glucose. HUVECs were incubated with the combined regime of 100nM Rapamycin and 30mM glucose over a period of 72h. The effect of rapamycin on cell viability, malondialdehyde levels, and nitric oxide were monitored by convenient assays. Intracellular ROS level was measured by flow cytometric analysis and DCFDA. HUVECs migration and angiogenic properties were assessed using scratch test and tubulogenesis assay. The expression of autophagic modulators LC3, Becline-1 and P62 was measured by using western blotting. Data showed 30mM glucose reduced cell viability, migration and in vitro tubulogenesis and level of ROS and nitric oxide were found to increased (p<0.05). Rapamycin had potential to increase cell survival and significantly decreased the total levels of oxidative stress markers after cell exposure to 30mM glucose (p<0.05). Rapamycin potentially improved the detrimental effect of 30mM glucose on cell migration and tubulogenesis capacity (p<0.05). Effective autophagic response was stimulated by rapamycin by increasing beclin-1, and the LC3-II/I ratio and reducing intracellular P62 level (p<0.05), resulting in the improvement of cell health and function. Together, rapamycin protected HUVECs from damages caused by high glucose concentration. This effect was possibly mediated by autophagy-dependent pathway. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

CMS MDS 3.0 Section M Skin Conditions in Long-term Care: Pressure Ulcers, Skin Tears, and Moisture-Associated Skin Damage Data Update.

PubMed

Ayello, Elizabeth A

2017-09-01

The purpose of this learning activity is to provide information about the updates to the Centers for Medicare & Medicaid Services (CMS) MDS 3.0 Section M, Skin Conditions documentation in long-term care. This continuing education activity is intended for physicians, physician assistants, nurse practitioners, and nurses with an interest in skin and wound care. After participating in this educational activity, the participant should be better able to:1. Explain the use of the CMS MDS 3.0 tool for documenting skin problems in long-term care.2. Demonstrate examples of proper documentation for specific skin problems. This manuscript reviews some of the key parts of the October 2016 revised Long-term Care Resident Assessment Instrument manual for Minimum Data Set (MDS) 3.0 Section M Skin Conditions. It also reports the Centers for Medicare & Medicaid's publicly reported frequency data in long-term care for selected items on the MDS 3.0 Section M Skin Conditions. Percentages and trends of pressure ulcers/injuries, skin tears, and moisture-associated skin damage are assessed.

Comparison of Efficacy and Safety of Liraglutide 3.0 mg in Individuals with BMI above and below 35 kg/m²: A Post-hoc Analysis.

PubMed

le Roux, Carel; Aroda, Vanita; Hemmingsson, Joanna; Cancino, Ana Paula; Christensen, Rune; Pi-Sunyer, Xavier

2017-01-01

To investigate whether the efficacy and safety of liraglutide 3.0 mg differed between two subgroups, BMI 27 to <35 and BMI ≥ 35 kg/m², in individuals without and with type 2 diabetes (T2D). A post-hoc analysis of two 56-week, randomized, double-blind, placebo-controlled trials (SCALE Obesity and Prediabetes; SCALE Diabetes). Subgroup differences in treatment effects of liraglutide 3.0 mg were evaluated by testing the interaction between treatment group and baseline BMI subgroup. Significantly greater weight loss (0-56 weeks) was observed with liraglutide 3.0 mg versus placebo in all patient groups while on treatment. There was no evidence that the weight-lowering effect of liraglutide 3.0 mg differed between BMI subgroups (interaction p > 0.05). Similarly, for most secondary endpoints significantly greater improvements were observed with liraglutide 3.0 mg versus placebo, with no indication treatment effects differing between subgroups. The safety profile of liraglutide 3.0 mg was broadly similar across BMI subgroups. This post-hoc analysis did not indicate any differences in the treatment effects, or safety profile, of liraglutide 3.0 mg for individuals with BMI 27 to <35 or ≥35 kg/m². Liraglutide 3.0 mg can therefore be considered for individuals with a BMI of ≥35 as well as for those with a BMI of 27 to <35 kg/m². © 2017 The Author(s) Published by S. Karger GmbH, Freiburg.

Evaluation of (99)  (m)TcN-MPO as a new myocardial perfusion imaging agent in normal dogs and in an acute myocardial infarction canine model: comparison with (99)  (m)Tc-sestamibi.

PubMed

Bu, Lihong; Li, Renfei; Jin, Zhongnan; Wen, Xiaofei; Liu, Shuang; Yang, Baofeng; Shen, Baozhong; Chen, Xiaoyuan

2011-02-01

(99)  (m)TcN-MPO ([(99)  (m)TcN(mpo)(PNP5)](+): mpo = 2-mercaptopyridine oxide and PNP5 = N-ethoxyethyl-N,N-bis[2-(bis(3-methoxypropyl)phosphino)ethyl]amine) is a cationic (99)  (m)Tc-nitrido complex, which has favorable biodistribution and myocardial uptake with rapid liver clearance in Sprague Dawley rats. The objective of this study was to compare the biodistribution and pharmacokinetics of (99)  (m)TcN-MPO and (99)  (m)Tc-Sestamibi in normal dogs, and to evaluate the potential of (99)  (m)TcN-MPO as a myocardial perfusion agent in canines with acute myocardial infarction. Five normal mongrel dogs were injected intravenously with (99)  (m)TcN-MPO. Venous blood samples were collected via a femoral vein catheter at 0.5, 1, 2, 3, 4, 5, 10, 20, 30, 40, 60, and 90 min post-injection (p.i.). Anterior-posterior planar images were acquired by γ-camera at 10, 20, 30, 60, 90, and 120 min p.i. Regions of interest (ROIs) were drawn around the heart, liver, and lungs. The heart/liver and heart/lung ratios were calculated by dividing the mean counts in heart ROI by the mean counts in the liver and lung ROI, respectively. For comparison, (99)  (m)Tc-sestamibi was also evaluated in the same five dogs. The interval period between the two examinations was 1 week to eliminate possible interference between these two radiotracers. In addition, single positron emission computed tomography (SPECT) images in the canine infarct model were collected 24 h after myocardial infarction at 30 and 60 min after the administration of (99)  (m)TcN-MPO (n = 4) or (99)  (m)Tc-Sestamibi (n = 4). It was found that (99)  (m)TcN-MPO and (99)  (m)Tc-Sestamibi displayed very similar blood clearance characteristics during the first 90 min p.i. Both (99)  (m)TcN-MPO and (99)  (m)Tc-Sestamibi had a rapid blood clearance with less than 50% of initial radioactivity remaining at 1 min and less than 5% at 30 min p.i. (99)  (m)TcN-MPO and (99)  (m)Tc-Sestamibi both showed good heart/lung contrast. The heart/liver ratio of (99)  (m)TcN-MPO increased with time (0.53 ± 0.06 at 10 min, 0.90 ± 0.062 at 30 min, and 1.22 ± 0.06 at 60 min p.i.), whereas the heart/liver ratio of (99)  (m)Tc-Sestamibi remained low at all time points (0.50 ± 0.03 at 10 min, 0.64 ± 0.03 at 30 min, and 0.60 ± 0.02 at 60 min p.i.). SPECT imaging studies in canines with acute myocardial infarction indicated that good visualization of the left ventricular wall and perfusion defects could be achieved at 30 min after administration of (99)  (m)TcN-MPO but not after (99)  (m)Tc-Sestamibi. The combination of reasonable heart uptake with rapid hepatobiliary excretion makes (99)  (m)TcN-MPO a promising new radiotracer for myocardial perfusion imaging.

High prevalence of Escherichia coli sequence type 131 among antimicrobial-resistant E. coli isolates from geriatric patients.

PubMed

Ho, Pak-Leung; Chu, Yuki Pui-Shan; Lo, Wai-U; Chow, Kin-Hung; Law, Pierra Y; Tse, Cindy Wing-Sze; Ng, Tak-Keung; Cheng, Vincent Chi-Chung; Que, Tak-Lun

2015-03-01

Previous work on the subclones within Escherichia coli ST131 predominantly involved isolates from Western countries. This study assessed the prevalence and antimicrobial resistance attributed to this clonal group. A total of 340 consecutive, non-duplicated urinary E. coli isolates originating from four clinical laboratories in Hong Kong in 2013 were tested. ST131 prevalence among the total isolates was 18.5 % (63/340) and was higher among inpatient isolates (23.0 %) than outpatient isolates (11.8 %, P<0.001), and higher among isolates from patients aged ≥65 years than from patients aged 18-50 years and 51-64 years (25.4 vs 3.4 and 4.0 %, respectively, P<0.001). Of the 63 ST131 isolates, 43 (68.3 %) isolates belonged to the H30 subclone, whereas the remaining isolates belonged to H41 (n = 17), H54 (n = 2) and H22 (n = 1). All H30 isolates were ciprofloxacin-resistant, of which 18.6 % (8/43) belonged to the H30-Rx subclone. Twenty-six (41.3 %) ST131 isolates were ESBL-producers, of which 19 had blaCTX-M-14 (12 non-H30-Rx, two H30-Rx and five H41), six had blaCTX-M-15 (five non-H30-Rx and one H30-Rx) and one was blaCTX-M-negative (H30). In conclusion, ST131 accounts for a large share of the antimicrobial-resistant E. coli isolates from geriatric patients. Unlike previous reports, ESBL-producing ST131 strains mainly belonged to non-H30-Rx rather than the H30-Rx subclone, with blaCTX-M-14 as the dominant enzyme type. © 2015 The Authors.

77 FR 69931 - Art Advisory Panel; Notice of Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-21

... 6, 2012, at 290 Broadway, Foley Square, New York, New York 10007 at 9:30 a.m. FOR FURTHER INFORMATION CONTACT: Ruth M. Vriend, C:AP:SO:ART, 1111 Constitution Ave. NW., Washington, DC 20024. Telephone... Advisory Panel will be held on December 6, 2012, at 290 Broadway, Foley Square at 9:30 a.m., New York, NY...

77 FR 61019 - Aquatic Nuisance Species Task Force Meeting

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2012-10-05

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33 CFR 117.736 - Oceanport Creek.

Code of Federal Regulations, 2010 CFR

2010-07-01

... except for Martin Luther King Day. Public vessels of the United States must be passed as soon as possible.... to 7:45 a.m. and 5:30 p.m. to 7:30 p.m on weekdays, excluding all federal holidays except for Martin Luther King Day. The drawspan must open on signal upon four hours notice from May 15 through September 15...

78 FR 25490 - Board of Governors; Sunshine Act Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-01

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Vehicle Test Facilities at Aberdeen Test Center and Yuma Test Center

DTIC Science & Technology

2012-02-27

boulders, concrete, steel 30.5 m 100 ft Corrosion Facility 27 Various corrosive environments NA NA Abrasive Mud Course 28 Sand loam 73 x 290 m 240 x...950 ft Fording Basin 30 Concrete 82 m 270 ft Underwater Fording Facility 31 Concrete 96 m 315 ft Amphibian Ramp 31 Bituminous concrete 6 x 15 m...Courses Fording Basin 97 Concrete (L x W) 67 x 25 m 220 x 82 ft Kofa Dust Course 98 Sand, Dust 3.2 km 2.0 mi Cibola Dust Course 99 Sand, Dust 6.3 km 3.9

Action potentials in primary osteoblasts and in the MG-63 osteoblast-like cell line.

PubMed

Pangalos, Maria; Bintig, Willem; Schlingmann, Barbara; Feyerabend, Frank; Witte, Frank; Begandt, Daniela; Heisterkamp, Alexander; Ngezahayo, Anaclet

2011-06-01

Whole-cell patch-clamp analysis revealed a resting membrane potential of -60 mV in primary osteoblasts and in the MG-63 osteoblast-like cells. Depolarization-induced action potentials were characterized by duration of 60 ms, a minimal peak-to-peak distance of 180 ms, a threshold value of -20 mV and a repolarization between the spikes to -45 mV. Expressed channels were characterized by application of voltage pulses between -150 mV and 90 mV in 10 mV steps, from a holding potential of -40 mV. Voltages below -60 mV induced an inward current. Depolarizing voltages above -30 mV evoked two currents: (a) a fast activated and inactivated inward current at voltages between -30 and 30 mV, and (b) a delayed-activated outward current that was induced by voltages above -30 mV. Electrophysiological and pharmacological parameters indicated that hyperpolarization activated strongly rectifying K(+) (K(ir)) channels, whereas depolarization activated tetrodotoxin sensitive voltage gated Na(+) (Na(v)) channels as well as delayed, slowly activated, non-inactivating, and tetraethylammonium sensitive voltage gated K(+) (K(v)) channels. In addition, RT-PCR showed expression of Na(v)1.3, Na(v)1.4, Na(v)1.5, Na(v)1.6, Na(v)1.7, and K(ir)2.1, K(ir)2.3, and K(ir)2.4 as well as K(v)2.1. We conclude that osteoblasts express channels that allow firing of action potentials.

78 FR 37244 - Proposal Review Panel for Ocean Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-20

... Meeting: Formal fourth year review of the Moorea Coral Reef Long-Term Ecological Research Project. Agenda... Presentations (open). 4:30 p.m.-5:30 p.m.--Demonstrations--Moorea Coral Reef (MCR) dry lab and wet lab (closed...

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2013-10-29

...: 8:30 a.m.-4:30 p.m. Eastern Time, November 15, 2013. Place: Teleconference. Status: The meeting is... matters, including the: (1) Review of extramural research concepts for funding opportunity announcements...

76 FR 70154 - National Heart, Lung, and Blood Institute; Notice of Closed Meetings

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2011-11-10

...: Endothelium and cardiovascular function. Date: December 2, 2011. Time: 8:30 a.m. to 4:30 p.m. Agenda: To... Vascular Diseases Research; 93.838, Lung Diseases Research; 93.839, Blood Diseases and Resources Research...

76 FR 12980 - Center for Scientific Review; Notice of Closed Meetings

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2011-03-09

... Emphasis Panel; Program Project: Cell Biology. Date: March 29-30, 2011. Time: 8 a.m. to 5 p.m. Agenda: To... Review Special Emphasis Panel; Program Project: NeuroAIDS Applications. Date: March 30-31, 2011. Time: 8...

76 FR 18512 - Shasta County Resource Advisory Committee

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2011-04-04

...) will meet at the USDA Service Center in Redding, California, on April 27, 2011, from 8:30 a.m. to 12... monitoring efforts for the upcoming year. DATES: Wednesday, April 27 at 8:30 a.m. ADDRESSES: The meeting will...

75 FR 18482 - Shasta County Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-12

...) will meet at the USDA Service Center in Redding, California, on April 28, 2010, from 8:30 a.m. to 12... monitoring efforts and a timeline for the upcoming year. DATES: Wednesday, April 28 at 8:30 a.m. ADDRESSES...

75 FR 11105 - Shasta County Resource Advisory Committee

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2010-03-10

...) will meet at the USDA Service Center in Redding, California, on March 31, 2010, from 8:30 a.m. to 12... monitoring efforts, and a timeline for the upcoming year. DATES: Wednesday, March 31 at 8:30 a.m. ADDRESSES...

76 FR 77268 - National Science Board; Sunshine Act Meetings; Notice Revised

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-12

... 2013 Budget Development Policies and planning for budget processes for FY 2014 and beyond Committee on... portfolio: getting from theory to scale Task Force on Merit Review (MR) Open Session: 2:30 p.m.-3:30 p.m...

78 FR 16273 - Center for Scientific Review; Notice of Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-14

...: Cardiovascular Clinical and Translational Studies Date: April 4, 2013. Time: 1:30 p.m. to 3:30 p.m. Agenda: To... Domestic Assistance Program Nos. 93.306, Comparative Medicine; 93.333, Clinical Research, 93.306, 93.333...

Florida Public Scoping Meeting | NOAA Gulf Spill Restoration

Science.gov Websites

Louisiana Mississippi Texas Region-wide Open Ocean Data Media & News Publications Press Releases Story open at 6:30 p.m., and the meeting will begin at 7:30 p.m. Bayview Community Center 2001 Lloyd Street

Women with a BMI ≥ 30kg/m² and their experience of maternity care: A meta ethnographic synthesis.

PubMed

Jones, Catriona; Jomeen, Julie

2017-10-01

this paper is a report of a systematic review and meta-ethnography of the experiences of women with body mass index (BMI) ≥ 30kg/m² and their experience of maternity care. systematic review methods identified 12 qualitative studies about women's experiences of maternity care when their BMI ≥ 30kg/m². Findings from the identified studies were synthesised into themes, using metaethnography. SYNTHESIS AND FINDINGS: the meta-ethnography produced four key concepts; Initial encounters, Negotiating risk, Missing out and The positive intervention, which represent the experiences of maternity care for women with BMI ≥ 30kg/m² KEY CONCLUSION: many women with BMI ≥ 30kg/m² appear to be dissatisfied with the approaches taken to discuss weight status during maternity encounters. When weight is not addressed during these encounters women appear to be equally dissatisfied. The absence of open and honest discussions about weight, the feeling of being denied of a normal experience, and an over emphasis on the risks imposed upon pregnancy and childbirth by obesity, leave women feeling dissatisfied and disenfranchised. Sensitive care and practical advice about diet and exercise can help women move towards feeling more in control of their weight management. Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

Field Testing New Plot Designs and Methods for Determining Hydrophytic Vegetation during Wetland Delineations in the United States

DTIC Science & Technology

2014-03-01

Trees and woody vines are sampled in large plots with 9 m (30 ft) radii. Saplings, shrubs , and herbs are sampled in nested smaller plots with 2 m (5 ft... woody vines in 9 m (30 ft) radius plots and saplings, shrubs , and herbaceous species in 2 m (5 ft) radius plots. In herbaceous meadows, only the 2 m (5...suggests stratifying vegetation by growth forms of trees, shrubs , herbs, and vines and sampling plant communities by using nested circular plots

Intramembraneous Particle Cluster and Cytoplasmic Vesicles in Mice with Nephrogenic Defects of Urinary Concentration.

DTIC Science & Technology

1987-01-01

DiBona , D.R., M.M. Civan, and A. Leaf. The cellular specificity of the effect of vasopressin on toad urinary bladder. J. Membr. Biol. 1:79-91, 1969. 30...Chem. 240:4524-4526, 1965. 62. Hardy, M.A., and D.R. DiBona . Microfilaments and the hydrosmotic action of vasopressin in toad urinary bladder. Am. J... DiBona , D.R., M.M. Civan, and A. Leaf. The cellular specificity of the effect of vasopressin on toad urinary bladder. J. Membr. Biol. 1:79-91, 1969. 30

Contrasting cardiovascular properties of the µ-opioid agonists morphine and methadone in the rat.

PubMed

Tung, Kenneth H; Angus, James A; Wright, Christine E

2015-09-05

Morphine and methadone share the property of μ-opioid receptor agonism yet have markedly different cardiovascular actions suggesting additional properties are at play. We investigated the i.v. dose-response relationships of the opioids on cardiovascular metameters in anaesthetised rats in the absence or presence of H1- and H2-receptor antagonism and the μ-opioid antagonist naloxone. In vitro tissue assays were employed to define more clearly cardiac and vascular mechanisms of action. Morphine (9, 30, 90mg/kg i.v.) decreased heart rate (HR) and mean arterial pressure (MAP) - responses that were blocked by naloxone pretreatment (10mg/kg i.v.). In contrast, methadone (3, 10, 30mg/kg i.v.) caused dramatic short-lived (1-3min) bradycardia, hypotension and lengthening of the QT interval before stabilising 5min after i.v. dosing. Only the steady-state responses of HR and MAP were blocked by naloxone. Mepyramine (10mg/kg i.v.) and cimetidine (100mg/kg i.v.) also blocked the naloxone-sensitive components. In isolated small mesenteric arteries precontracted by K(+) 62mM or endothelin-1, methadone (1-30μM) relaxed vessels while morphine (1-100μM) had no effect. Pretreatment with naloxone (10μM), indomethacin (30μM) or nitro-l-arginine (100μM) did not affect the relaxation to methadone. In rat isolated left atria, morphine and methadone inhibited inotropic responses at high concentrations (100μM). In rat papillary muscle and right atria, methadone was more than 30 times more potent at lengthening the refractory period and slowing the atrial rate than morphine. We conclude that methadone is a potent vasodilator agent, possibly through blocking L-type calcium channels. Copyright © 2015 Elsevier B.V. All rights reserved.

Relationships and Predictive Capabilities of Jump Assessments to Soccer-Specific Field Test Performance in Division I Collegiate Players

PubMed Central

Lockie, Robert G.; Stage, Alyssa A.; Stokes, John J.; Orjalo, Ashley J.; Davis, DeShaun L.; Giuliano, Dominic V.; Moreno, Matthew R.; Risso, Fabrice G.; Lazar, Adrina; Birmingham-Babauta, Samantha A.; Tomita, Tricia M.

2016-01-01

Leg power is an important characteristic for soccer, and jump tests can measure this capacity. Limited research has analyzed relationships between jumping and soccer-specific field test performance in collegiate male players. Nineteen Division I players completed tests of: leg power (vertical jump (VJ), standing broad jump (SBJ), left- and right-leg triple hop (TH)); linear (30 m sprint; 0–5 m, 5–10 m, 0–10, 0–30 m intervals) and change-of-direction (505) speed; soccer-specific fitness (Yo-Yo Intermittent Recovery Test Level 2); and 7 × 30-m sprints to measure repeated-sprint ability (RSA; total time (TT), performance decrement (PD)). Pearson’s correlations (r) determined jump and field test relationships; stepwise regression ascertained jump predictors of the tests (p < 0.05). All jumps correlated with the 0–5, 0–10, and 0–30 m sprint intervals (r = −0.65–−0.90). VJ, SBJ, and left- and right-leg TH correlated with RSA TT (r = −0.51–−0.59). Right-leg TH predicted the 0–5 and 0–10 m intervals (R2 = 0.55–0.81); the VJ predicted the 0–30 m interval and RSA TT (R2 = 0.41–0.84). Between-leg TH asymmetry correlated with and predicted left-leg 505 and RSA PD (r = −0.68–0.62; R2 = 0.39–0.46). Improvements in jumping ability could contribute to faster speed and RSA performance in collegiate soccer players. PMID:29910304

Psychometric properties of the 30-m walking test in patients with degenerative cervical myelopathy: results from two prospective multicenter cohort studies.

PubMed

Bohm, Parker E; Fehlings, Michael G; Kopjar, Branko; Tetreault, Lindsay A; Vaccaro, Alexander R; Anderson, Karen K; Arnold, Paul M

2017-02-01

The timed 30-m walking test (30MWT) is used in clinical practice and in research to objectively quantify gait impairment. The psychometric properties of 30MWT have not yet been rigorously evaluated. This study aimed to determine test-retest reliability, divergent and convergent validity, and responsiveness to change of the 30MWT in patients with degenerative cervical myelopathy (DCM). A retrospective observational study was carried out. The sample consisted of patients with symptomatic DCM enrolled in the AOSpine North America or AOSpine International cervical spondylotic myelopathy studies at 26 sites. Modified Japanese Orthopaedic Association scale (mJOA), Nurick scale, 30MWT, Neck Disability Index (NDI), and Short-Form-36 (SF-36v2) physical component score (PCS) and mental component score (MCS) were the outcome measures. Data from two prospective multicenter cohort myelopathy studies were merged. Each patient was evaluated at baseline and 6 months postoperatively. Of 757 total patients, 682 (90.09%) attempted to perform the 30MWT at baseline. Of these 682 patients, 602 (88.12%) performed the 30MWT at baseline. One patient was excluded, leaving601 in the analysis. At baseline, 81 of 682 (11.88%) patients were unable to perform the test, and their mJOA, NDI, and SF-36v2 PCS scores were lower compared with those who performed the test at baseline. In patients who performed the 30MWT at baseline, there was very high correlation among the three baseline 30MWT measurements (r=0.9569-0.9919). The 30MWT demonstrated good convergent and divergent validity. It was moderately correlated with the Nurick (r=0.4932), mJOA (r=-0.4424), and SF-36v2 PCS (r=-0.3537) (convergent validity) and poorly correlated with the NDI (r=0.2107) and SF-36v2 MCS (r=-0.1984) (divergent validity). Overall, the 30MWT was not responsive to change (standardized response mean [SRM]=0.30). However, for patients who had a baseline time above the median value of 29 seconds, the SRM was 0.45. The 30MWT shows high test-retest reliability and good divergent and convergent validity. It is responsive to change only in patients with more severe myelopathy. The 30MWT is a simple, quick, and affordable test, and should be used as an ancillary test to evaluate gait parameters in patients with DCM. Copyright © 2016 Elsevier Inc. All rights reserved.

40 CFR Table 30 to Subpart G of... - Roof Fitting Loss Factors, KFa, KFb, and m, a and Typical Number of Fittings, NT

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 10 2013-07-01 2013-07-01 false Roof Fitting Loss Factors, KFa, KFb, and m, a and Typical Number of Fittings, NT 30 Table 30 to Subpart G of Part 63 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE...

40 CFR Table 30 to Subpart G of... - Roof Fitting Loss Factors, KFa, KFb, and m, a and Typical Number of Fittings, NT

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 10 2012-07-01 2012-07-01 false Roof Fitting Loss Factors, KFa, KFb, and m, a and Typical Number of Fittings, NT 30 Table 30 to Subpart G of Part 63 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE...

40 CFR Table 30 to Subpart G of... - Roof Fitting Loss Factors, KFa, KFb, and m, a and Typical Number of Fittings, NT

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 10 2014-07-01 2014-07-01 false Roof Fitting Loss Factors, KFa, KFb, and m, a and Typical Number of Fittings, NT 30 Table 30 to Subpart G of Part 63 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE...

40 CFR Table 30 to Subpart G of... - Roof Fitting Loss Factors, KFa, KFb, and m, a and Typical Number of Fittings, NT

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 9 2011-07-01 2011-07-01 false Roof Fitting Loss Factors, KFa, KFb, and m, a and Typical Number of Fittings, NT 30 Table 30 to Subpart G of Part 63 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE...

40 CFR Table 30 to Subpart G of... - Roof Fitting Loss Factors, KFa, KFb, and m, a and Typical Number of Fittings, NT

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 9 2010-07-01 2010-07-01 false Roof Fitting Loss Factors, KFa, KFb, and m, a and Typical Number of Fittings, NT 30 Table 30 to Subpart G of Part 63 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE...

Reduction of non-Betalactam Antibiotics COD by Combined Coagulation and Advanced Oxidation Processes.

PubMed

Yazdanbakhsh, Ahmad Reza; Mohammadi, Amir Sheikh; Alinejad, Abdol Azim; Hassani, Ghasem; Golmohammadi, Sohrab; Mohseni, Seyed Mohsen; Sardar, Mahdieh; Sarsangi, Vali

2016-11-01

  The present study evaluates the reduction of antibiotic COD from wastewater by combined coagulation and advanced oxidation processes (AOPS). The reduction of Azithromycin COD by combined coagulation and Fenton-like processes reached a maximum 96.9% at a reaction time of 30 min, dosage of ferric chloride 120 mg/L, dosages of Fe0 and H2O2of 0.36mM/L and 0.38 mM/L, respectively. Also, 97.9% of Clarithromycin COD reduction, was achieved at a reaction time of 30 min, dosage of ferric chloride 120 mg/L, dosages of Fe0 and H2O2 of 0.3 mM/L and 0.3mM/L, respectively. The results of kinetic studies were best fitted to the pseudo first order equation. The results showed a higher rate constant value for combined coagulation and Fenton-like processes [(kap = 0.022 min-1 and half-life time of 31.5 min for Azithromycin) and (kap = 0.023 min-1 and half-life time of 30.1 min for Clarithromycin)].

Effects of sildenafil on cardiac repolarization.

PubMed

Chiang, Chern-En; Luk, Hsiang-Ning; Wang, Tsui-Min; Ding, Philip Yu-An

2002-08-01

Sudden death has occasionally been reported in patients taking sildenafil. The objective of this study was to investigate the effect of sildenafil on cardiac repolarization. We used conventional microelectrode recording technique in isolated guinea pig papillary muscles and canine Purkinje fibers, whole-cell patch clamp techniques in guinea pig ventricular myocytes, and in vivo ECG measurements in guinea pigs. Action potential duration at 90% repolarization (APD(90)) was not affected by sildenafil in the therapeutic ranges (< or =1 microM), but shortened by higher concentration (> or =10 microM) in both guinea pig papillary muscles and canine Purkinje fibers. D-Sotalol prolonged APD(90) in the same preparations with concentrations > or =1 microM in a reverse frequency-dependent manner. Co-administration of sildenafil (10 and 30 microM) abolished the APD-prolonging effects of D-sotalol (30 microM) and amiodarone (100 microM). Sildenafil, with concentrations up to 30 microM, had no significant effect on both the rapid (I(Kr)) and the slow (I(Ks)) components of the delayed rectifier potassium currents in guinea pig ventricular myocytes. Sildenafil dose-dependently blocked L-type Ca(2+) current (I(Ca,L)), but had no effect on persistent Na(+) current in guinea pig ventricular myocytes. ECG recordings in intact guinea pigs revealed significant shortening of QTc interval by sildenafil (10 and 30 mg/kg orally). The QT-prolonging effects by D,L-sotalol (50 mg/kg) and amiodarone (100 mg/kg) were abolished by sildenafil (30 mg/kg). Sildenafil does not prolong cardiac repolarization. Instead, in supra-therapeutic concentrations, it accelerates cardiac repolarization, presumably through its blocking effect on I(Ca,L).

Remote Sensing of Wildland Fire-Induced Risk Assessment at the Community Level.

PubMed

Ahmed, M Razu; Rahaman, Khan Rubayet; Hassan, Quazi K

2018-05-15

Wildland fires are some of the critical natural hazards that pose a significant threat to the communities located in the vicinity of forested/vegetated areas. In this paper, our overall objective was to study the structural damages due to the 2016 Horse River Fire (HRF) that happened in Fort McMurray (Alberta, Canada) by employing primarily very high spatial resolution optical satellite data, i.e., WorldView-2. Thus, our activities included the: (i) estimation of the structural damages; and (ii) delineation of the wildland-urban interface (WUI) and its associated buffers at certain intervals, and their utilization in assessing potential risks. Our proposed method of remote sensing-based estimates of the number of structural damages was compared with the ground-based information available from the Planning and Development Recovery Committee Task Force of Regional Municipality of Wood Buffalo (RMWB); and found a strong linear relationship (i.e., r² value of 0.97 with a slope of 0.97). Upon delineating the WUI and its associated buffer zones at 10 m, 30 m, 50 m, 70 m and 100 m distances; we found existence of vegetation within the 30 m buffers from the WUI for all of the damaged structures. In addition, we noticed that the relevant authorities had removed vegetation in some areas between 30 m and 70 m buffers from the WUI, which was proven to be effective in order to protect the structures in the adjacent communities. Furthermore, we mapped the wildland fire-induced vulnerable areas upon considering the WUI and its associated buffers. Our analysis revealed that approximately 30% of the areas within the buffer zones of 10 m and 30 m were vulnerable due to the presence of vegetation; in which, approximately 7% were burned during the 2016 HRF event that led the structural damages. Consequently, we suggest to remove the existing vegetation within these critical zones and also monitor the region at a regular interval in order to reduce the wildland fire-induced risk.

Speed and agility of 12- and 14-year-old elite male basketball players.

PubMed

Jakovljevic, Sasa T; Karalejic, Milivoje S; Pajic, Zoran B; Macura, Marija M; Erculj, Frane F

2012-09-01

The aims of this study were (a) to identify and compare the speed and agility of 12- and 14-year-old elite male basketball players and (b) to investigate relations between speed and agility for both age groups of basketball players, to help coaches to improve their work. Sixty-four players aged 12 (M = 11.98 years, SD = 0.311) and 54 players aged 14 (M = 14.092 years, SD = 0.275) were tested. Three agility tests: agility t-test, zigzag agility drill, and agility run 4 × 15 m and 3 speed tests: 20-m run, 30-m run, and 50-m run were applied. Fourteen-year-old players achieved significantly better results in all speed and agility tests compared with 12-year-old players. The correlation coefficient (r = 0.81, p = 0.001) showed that 12-year-old players have the same ability in the 30- and 50-m runs. The other correlation coefficient (r = 0.59, p = 0.001) indicated that 20- and 30-m runs had inherently different qualities. The correlation coefficients between agility tests were <0.71, and therefore, each test in this group represents a specific task. In 14-year-old players, the correlation coefficients between the speed test results were <0.71. In contrast, the correlation coefficients between the agility tests were >0.71, which means that all the 3 tests represent the same quality. During the speed training of 12-year-old players, it is advisable to focus on shorter running distances, up to 30 m. During the agility training of the same players, it is useful to apply exercises with various complexities. In speed training of the 14-year-old players, the 30- and 50-m runs should be applied, and agility training should include more specific basketball movements and activities.

Silicon isotope fractionation in rice and cucumber plants over a life cycle: Laboratory studies at different external silicon concentrations

NASA Astrophysics Data System (ADS)

Sun, Yan; Wu, Lianghuan; Li, Xiaoyan; Sun, Li; Gao, Jianfei; Ding, Tiping

2016-11-01

Understanding the variations of silicon isotopes in terrestrial higher plants can be helpful toward elucidating the global biogeochemical silicon cycle. We studied silicon isotope fractionation in rice and cucumber plants over their entire life cycles. These two different silicon-absorbing plants were grown hydroponically at different external silicon concentrations. The ranges of δ30Si values in rice were -1.89‰ to 1.69‰, -1.81‰ to 1.96‰, and -2.08‰ to 2.02‰ at 0.17 mM, 1.70 mM, and 8.50 mM silicon concentrations, respectively. The ranges of δ30Si values in cucumber were -1.38‰ to 1.21‰, -1.33‰ to 1.26‰, and -1.62‰ to 1.40‰ at 0.085 mM, 0.17 mM, and 1.70 mM external silicon concentrations, respectively. A general increasing trend in δ30Si values from lower to upper plant parts reflected the preferential incorporation of lighter silicon isotopes from transpired water to biogenic opal. Furthermore, the active uptake mechanism regulated by several transporters might have also played an important role in the preferential transport of heavy silicon isotopes into aboveground plant parts. This suggested that silicon isotope fractionation in both rice and cucumber was a Rayleigh-like process. The data on δ30Si values for the whole plants and nutrient solutions indicated that biologically mediated silicon isotope fractionation occurred during silicon uptake by roots. At lower external silicon concentrations, heavy silicon isotopes entered plants more readily than light silicon isotopes. Conversely, at higher external silicon concentrations, light silicon isotopes entered plants more readily than heavy silicon isotopes.

Carbonic anhydrase 2-like and Na⁺-K⁺-ATPase α gene expression in medaka (Oryzias latipes) under carbonate alkalinity stress.

PubMed

Yao, Zongli; Lai, Qifang; Hao, Zhuoran; Chen, Ling; Lin, Tingting; Zhou, Kai; Wang, Hui

2015-12-01

High carbonate alkalinity is one of the major stress factors for living organisms in saline-alkaline water areas. Acute and chronic effects of carbonate alkalinity on expression of two genes, carbonic anhydrase 2-like (CA2-like) and Na(+)-K(+)-ATPase α subunit (NKA-α) mRNA in medaka (Oryzias latipes) were evaluated to better understand the responses important for coping with a carbonate alkalinity stress. In the acute exposure experiment, the expression of CA2-like and NKA-α mRNA in the gill and kidney of medaka were examined from 0 h to 7 days exposed to 30.4 mM carbonate alkalinity water. Exposure to high carbonate alkalinity resulted in a transitory alkalosis, followed by a transient increase in gill and kidney CA2-like and NKA-α mRNA expression. In the chronic exposure experiment, the expression of these two genes was examined in the gill and kidney at 50 days post-exposure to six different carbonate alkalinity concentrations ranging from 1.5 to 30.4 mM. Gill and kidney CA2-like mRNA levels in 30.4 mM were approximately 10 and 30 times higher than that of the control (1.5 mM), respectively. Less differences were found in NKA-α expression in the 50-days exposure. The results indicate that when transferred to high carbonate alkalinity water, a transitory alkalosis may occur in medaka, followed by compensatory acid-base and ion regulatory responses. Thus, CA2-like and NKA-α are at least two of the important factors that contribute to the regulation of alkalinity stress.

Artificial neural network-based model for the prediction of optimal growth and culture conditions for maximum biomass accumulation in multiple shoot cultures of Centella asiatica.

PubMed

Prasad, Archana; Prakash, Om; Mehrotra, Shakti; Khan, Feroz; Mathur, Ajay Kumar; Mathur, Archana

2017-01-01

An artificial neural network (ANN)-based modelling approach is used to determine the synergistic effect of five major components of growth medium (Mg, Cu, Zn, nitrate and sucrose) on improved in vitro biomass yield in multiple shoot cultures of Centella asiatica. The back propagation neural network (BPNN) was employed to predict optimal biomass accumulation in terms of growth index over a defined culture duration of 35 days. The four variable concentrations of five media components, i.e. MgSO 4 (0, 0.75, 1.5, 3.0 mM), ZnSO 4 (0, 15, 30, 60 μM), CuSO 4 (0, 0.05, 0.1, 0.2 μM), NO 3 (20, 30, 40, 60 mM) and sucrose (1, 3, 5, 7 %, w/v) were taken as inputs for the ANN model. The designed model was evaluated by performing three different sets of validation experiments that indicated a greater similarity between the target and predicted dataset. The results of the modelling experiment suggested that 1.5 mM Mg, 30 μM Zn, 0.1 μM Cu, 40 mM NO 3 and 6 % (w/v) sucrose were the respective optimal concentrations of the tested medium components for achieving maximum growth index of 1654.46 with high centelloside yield (62.37 mg DW/culture) in the cultured multiple shoots. This study can facilitate the generation of higher biomass of uniform, clean, good quality C. asiatica herb that can efficiently be utilized by pharmaceutical industries.

75 FR 39192 - Airworthiness Directives; Arrow Falcon Exporters, Inc. (Previously Utah State University), et al...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-08

... Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue, SE., Washington, DC 20590. Hand Delivery: U.S. Department of Transportation, Docket Operations, M-30, West Building Ground Floor... [[Page 39193

76 FR 33771 - Eunice Kennedy Shriver National Institute of Child Health & Human Development; Notice of Closed...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-09

... Institute of Child Health and Human Development Special Emphasis Panel, Intellectual and Developmental Disabilities Research Centers 2011 (P30) Review. Date: June 29-30, 2011. Time: 8 a.m. to 6 p.m. Agenda: To...

77 FR 14027 - National Institute of Diabetes and Digestive and Kidney Diseases Diabetes Mellitus Interagency...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-08

..., 2012, from 8:30 a.m. to 11:30 a.m. at the Neuroscience Building, Room C, 6001 Executive Boulevard... year, provide an opportunity for members to learn about and discuss current and future diabetes...

75 FR 66757 - Stakeholder Input; Listening Session Seeking Suggestions for Improving the Next National...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-29

..., 1301 Constitution Ave., NW., Washington, DC. The Public Reading Room is open from 8:30 a.m. to 4:30 p.m... leachate from anti-fouling hull coatings; aqueous film forming foam (AFFF); boiler/economizer blowdown...

78 FR 36034 - Citizens Coinage Advisory Committee; Public Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-14

.... Time: 9:30 a.m. to 2:30 p.m. Location: Conference Room A, United States Mint, 801 9th Street NW... the CCAC; 801 9th Street NW.; Washington, DC 20220; or call 202-354-7200. Any member of the public...

78 FR 59426 - Citizens Coinage Advisory Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-26

.... Time: 9:30 a.m. to 1:30 p.m. Location: Conference Room A, United States Mint, 801 9th Street NW... the CCAC; 801 9th Street NW.; Washington, DC 20220; or call 202-354-7200. Any member of the public...

76 FR 58077 - RTCA Program Management Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-19

... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration RTCA Program Management Committee AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of RTCA Program Management Committee... Management Committee. DATES: The meeting will be held September 28, 2011 from 8:30 a.m. to 1:30 p.m...

75 FR 52590 - RTCA Program Management Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-26

... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration RTCA Program Management Committee AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of RTCA Program Management Committee... Management Committee. DATES: The meeting will be held September 15, 2010 from 8:30 a.m. to 1:30 p.m...

76 FR 66731 - Office of the Director, National Institutes of Health, Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-27

...:30 p.m. to 3:30 p.m. Agenda: The Council will receive information on the Obesity Initiative, Science Education and new initiatives at NIH. Place: National Institutes of Health, Building 31, C Wing, 6th Floor...

76 FR 9223 - Drawbridge Operation Regulation; Upper Mississippi River, Rock Island, IL

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-17

... is necessary to allow the Quad City Marathon to cross the bridge. This deviation allows the bridge to...:30 a.m. to 11:30 a.m., September 25, 2011, while a marathon is held between the cities of Davenport...

77 FR 5398 - Drawbridge Operation Regulation; Upper Mississippi River, Rock Island, IL

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-03

... is necessary to allow the Quad City Marathon to cross the bridge. This deviation allows the bridge to...:30 a.m. to 11:30 a.m., September 23, 2012, while a marathon is held between the cities of Davenport...

77 FR 66619 - Partnerships To Advance the National Occupational Research Agenda (NORA)

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-06

...)''. Public Meeting Time and Date: 10 a.m.-3:30 p.m. EST, January 30, 2013. Place: Patriots Plaza, 395 E... that is working on several NORA priorities may also provide an update. After each update, there will be...

Toxicology of oxides of nitrogen. I. Toxic concentrations

DOE Office of Scientific and Technical Information (OSTI.GOV)

La Towsky, L.W.; MacQuiddy, E.L.; Tollman, J.P.

1941-01-01

One hundred twelve rabbits, cats, guinea pigs, rats, and mice were exposed to 30 to 1000 ppM NO/sub 2/. Average time to produce death was: 30 ppM - none in 3 hr, 100 ppM - 318 min, 150 ppM - 90 min, 400 ppM - 58 min, 600 ppM - 32 min, 800 ppM - 29 min, 1000 ppM - 19 min. Rats and mice were more sensitive, sometimes exhibiting delayed effects (e.g., 2 to 3 hr at 55 ppM). Cats were most sensitive in that they thrash excitedly, thus breathing more. Methemoglobin was frequently seen in blood during exposure.more » Death was from edema (most common), asphyxia, or pneumonitis (secondary).« less

Calcium deprivation increases the palatability of calcium solutions in rats.

PubMed

McCaughey, Stuart A; Forestell, Catherine A; Tordoff, Michael G

2005-02-15

Calcium-deprived rats have elevated intakes of CaCl2, other calcium salts, and some non-calcium compounds. We used taste reactivity to examine the effects of calcium deprivation on the palatability of CaCl2 and other solutions. Nine male Sprague-Dawley rats were calcium-deprived by maintenance on a low-calcium diet, and eight replete rats were used as controls. All rats were videotaped during intraoral infusion of the following solutions: 30 and 300 mM CaCl2, 30 mM calcium lactate, 100 and 600 mM NaCl, 30 mM MgCl2, 1 mM quinine.HCl, 2.5 mM sodium saccharin, and deionized water. We counted individual orofacial and somatic movements elicited by the infusions and used them to calculate total ingestive and aversive scores. Relative to controls, calcium-deprived rats gave a significantly larger number of tongue protrusions and had higher total ingestive scores for CaCl2, calcium lactate, NaCl, and MgCl2. Our results suggest that CaCl2, calcium lactate, NaCl, and MgCl2 taste more palatable to rats when they are calcium-deprived than replete, and this may be responsible for the increased intake of these solutions following calcium deprivation.

Prediction of presence of kidney disease in a general patient population undergoing intravenous iodinated contrast enhanced computed tomography.

PubMed

Moos, Shira I; Stoker, Jaap; Nagan, Gajenthiran; de Weijert, Roderick S; van Vemde, David N H; Bipat, Shandra

2014-06-01

To assess which risk factors can be used to reduce superfluous estimated glomerular filtration rate (eGFR) measurements before intravenous contrast medium administration. In consecutive patients, all decreased eGFR risk factors were assessed: diabetes mellitus (DM), history of urologic/nephrologic disease (HUND), nephrotoxic medication, cardiovascular disease, hypertension, age > 60 years, anaemia, malignancy and multiple myeloma/M. Waldenström. We studied four models: (1) all risk factors, (2) DM, HUND, hypertension, age > 60 years; (3) DM, HUND, cardiovascular disease, hypertension; (4) DM, HUND, age > 75 years and congestive heart failure. For each model, association with eGFR < 60 ml/min/1.73 m(2) or eGFR < 45 ml/min/1.73 m(2) was studied. A total of 998 patients, mean age 59.94 years were included; 112 with eGFR < 60 ml/min/1.73 m(2) and 30 with eGFR < 45 ml/min/1.73 m(2). Model 1 detected 816 patients: 108 with eGFR < 60 ml/min/1.73 m(2) and all 30 with eGFR < 45 ml/min/1.73 m(2). Model 2 detected 745 patients: 108 with eGFR < 60 ml/min/1.73 m(2) and all 30 with eGFR < 45 ml/min/1.73 m(2). Model 3 detected 622 patients: 100 with eGFR < 60 ml/min/1.73 m(2) and all 30 with eGFR < 45 ml/min/1.73 m(2). Model 4 detected 440 patients: 86 with eGFR < 60 ml/min/1.73 m(2) and all 30 with eGFR < 45 ml/min/1.73 m(2). Associations were significant (p < 0.001). Model 4 is most effective, resulting in the lowest proportion of superfluous eGFR measurements while detecting all patients with eGFR < 45 ml/min/1.73 m(2) and most with eGFR < 60 ml/min/1.73 m(2). A major risk factor for contrast-induced nephropathy (CIN) is kidney disease. Risk factors are used to identify patients with pre-existent kidney disease. Evidence for risk factors to identify patients with kidney disease is limited. The number of eGFR measurements to detect kidney disease can be reduced.

78 FR 938 - Notification of a Public Meeting and a Public Teleconference of the Clean Air Scientific Advisory...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-07

.... to 4:00 p.m. (Eastern Time). ADDRESSES: The CASAC Lead Review Panel face-to-face public meeting will... Quality Standards (First External Review Draft--January 2013). DATES: The CASAC Lead Review Panel face-to-face public meeting will be held on Tuesday, February 5, 2013, from 8:30 a.m. to 5:30 p.m. (Eastern...

75 FR 19652 - National Advisory Council on Migrant Health; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-15

... Advisory Council on Migrant Health; Notice of Meeting In accordance with section 10(a)(2) of the Federal... Advisory Council on Migrant Health. Dates and Times: May 3, 2010, 8:30 a.m. to 5 p.m. May 4, 2010, 8:30 a.m... of the meeting is to discuss services and issues related to the health of migrant and seasonal...

76 FR 39774 - Drawbridge Operation Regulation; Black Warrior River, Demopolis, Marengo County, AL

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-07

.... Frank, Chief, Bridge Administration Branch, U.S. Coast Guard. [FR Doc. 2011-17106 Filed 7-6-11; 8:45 am... issued a temporary deviation from the regulation governing the operation of the Yo-Yo vertical lift span... to navigation from 8 a.m. through 10:30 a.m. and then from noon through 2:30 p.m. each day for 6...

29 CFR 1915.158 - Lifesaving equipment.

Code of Federal Regulations, 2014 CFR

2014-07-01

... floating vessels under 200 feet (61 m) in length, at least one 30-inch (0.76 m) U.S. Coast Guard approved ring life buoy with line attached shall be located at the gangway. (3) At least one 30-inch (0.76 m) U... pursuant to 46 CFR part 160 (Type I, II, III, or V PFD) and marked for use as a work vest, for commercial...

29 CFR 1915.158 - Lifesaving equipment.

Code of Federal Regulations, 2012 CFR

2012-07-01

... floating vessels under 200 feet (61 m) in length, at least one 30-inch (0.76 m) U.S. Coast Guard approved ring life buoy with line attached shall be located at the gangway. (3) At least one 30-inch (0.76 m) U... pursuant to 46 CFR part 160 (Type I, II, III, or V PFD) and marked for use as a work vest, for commercial...

29 CFR 1915.158 - Lifesaving equipment.

Code of Federal Regulations, 2013 CFR

2013-07-01

... floating vessels under 200 feet (61 m) in length, at least one 30-inch (0.76 m) U.S. Coast Guard approved ring life buoy with line attached shall be located at the gangway. (3) At least one 30-inch (0.76 m) U... pursuant to 46 CFR part 160 (Type I, II, III, or V PFD) and marked for use as a work vest, for commercial...

75 FR 22724 - Drawbridge Operation Regulation; Chambers Creek, Steilacoom, WA, Schedule Change

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-30

... which two-hour notice would have been required for openings from 3:30 p.m. to 7 a.m. every day. The NPRM.... The primary point of objection was that 3:30 p.m. was too early in the day, especially in summer... the end of a day of boating. The Coast Guard received a total of 17 written responses. At least seven...

76 FR 34867 - Safety Zones; Annual Fireworks Events in the Captain of the Port Detroit Zone

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-15

....941(a)(30) Bay-Rama Fishfly Festival Fireworks, New Baltimore, MI This safety zone will be enforced...) Bay City Fireworks Festival, Bay City, MI This safety zone will be enforced daily from 9:30 p.m. to 11...:45 p.m. to 10:45 p.m. on July 3, 2011. Section 165.941(a)(43) Lexington Independence Festival...

75 FR 35652 - Safety Zone; Northern California Annual Fireworks Events, Fourth of July Fireworks, South Lake...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-23

... Zone; Northern California Annual Fireworks Events, Fourth of July Fireworks, South Lake Tahoe Gaming... will enforce Lights on the Lake Fireworks Display safety zone for South Lake Tahoe, from 8:30 a.m. on... the Lake Fireworks in 33 CFR 165.1191 on July 4, 2010, from 8:30 a.m. on July 1, 2010 through 10 p.m...

17 CFR 260.0-5 - Business hours of the Commission.

Code of Federal Regulations, 2011 CFR

2011-04-01

...., Washington, DC 20549, is open each day, except Saturdays, Sundays and federal holidays, from 9 a.m. to 5:30 p... federal holidays, from 8 a.m. to 5:30 p.m., Eastern Standard Time or Eastern Daylight Saving Time.... 18, 1993, as amended at 65 FR 24802, Apr. 27, 2000; 68 FR 25800, May 13, 2003; 73 FR 32228, June 5...

17 CFR 260.0-5 - Business hours of the Commission.

Code of Federal Regulations, 2012 CFR

2012-04-01

...., Washington, DC 20549, is open each day, except Saturdays, Sundays and federal holidays, from 9 a.m. to 5:30 p... federal holidays, from 8 a.m. to 5:30 p.m., Eastern Standard Time or Eastern Daylight Saving Time.... 18, 1993, as amended at 65 FR 24802, Apr. 27, 2000; 68 FR 25800, May 13, 2003; 73 FR 32228, June 5...

17 CFR 260.0-5 - Business hours of the Commission.

Code of Federal Regulations, 2014 CFR

2014-04-01

...., Washington, DC 20549, is open each day, except Saturdays, Sundays and federal holidays, from 9 a.m. to 5:30 p... federal holidays, from 8 a.m. to 5:30 p.m., Eastern Standard Time or Eastern Daylight Saving Time.... 18, 1993, as amended at 65 FR 24802, Apr. 27, 2000; 68 FR 25800, May 13, 2003; 73 FR 32228, June 5...

17 CFR 260.0-5 - Business hours of the Commission.

Code of Federal Regulations, 2013 CFR

2013-04-01

...., Washington, DC 20549, is open each day, except Saturdays, Sundays and federal holidays, from 9 a.m. to 5:30 p... federal holidays, from 8 a.m. to 5:30 p.m., Eastern Standard Time or Eastern Daylight Saving Time.... 18, 1993, as amended at 65 FR 24802, Apr. 27, 2000; 68 FR 25800, May 13, 2003; 73 FR 32228, June 5...

78 FR 27256 - Meeting of the California Desert District Advisory Council

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-09

..., from 8 a.m. to 4:30 p.m. in Ridgecrest, Calif. at a location to be announced later. There also will be a DAC Business Meeting on Friday, June 7, from noon to 4:30 p.m. at the Jawbone Station Visitors... Saturday meeting will include a focus on the West Mojave Plan, as well as updates by council members, the...

78 FR 79026 - Board of Governors; Sunshine Act Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-27

... POSTAL SERVICE Board of Governors; Sunshine Act Meeting DATES AND TIMES: January 7, 2014, at 2:30 p.m., and January 8, 2014, at 7:15 a.m. PLACE: Las Vegas, Nevada. STATUS: Closed. Matters To Be Considered Tuesday, January 7, 2014, at 2:30 p.m. 1. Strategic Issues. 2. Pricing. 3. Governors' Executive Session. Wednesday, January 8, 2014, at 7:15...

Maintenance of platelet in vitro properties during 7-day storage in M-sol with a 30-hour interruption of agitation.

PubMed

Wagner, Stephen J; Myrup, Andrew; Awatefe, Helen; Thompson-Montgomery, Dedeene; Hirayama, Junichi; Skripchenko, Andrey

2008-12-01

Extensive periods without agitation can occasionally occur during platelet (PLT) shipment and can affect PLT quality during 5- to 7-day storage. The use of buffer-containing PLT additive solutions (ASs) may better preserve PLT quality during storage by maintaining PLT pH and other in vitro variables. A newly described bicarbonate-containing AS, M-sol, was compared to plasma for preservation of whole blood-derived PLT concentrates in which a 30-hour interruption of agitation was included. ABO-identical PLT-rich plasma intermediate products were pooled in sets of four, split, and centrifuged with subsequent plasma expression (n = 12). Two units were resuspended with M-sol AS to produce a 70 percent solution/30 percent plasma PLT concentrate; 2 units were resuspended in 100 percent plasma. One M-sol resuspended unit and 1 plasma unit were held on a laboratory bench in a standard shipping box for 30 hours between Day 2 and Day 3, while the other M-sol and plasma unit were continuously agitated. Standard in vitro testing for PLT quality variables on each set of 4 units was performed during storage (n = 12). Interrupting agitation of PLTs suspended in M-sol resulted in less of a pH decrement during storage than that of PLTs suspended in 100 percent plasma. On Days 5 and 7, the pH differences between M-sol and plasma units were 0.56 and 0.75 pH units, respectively (p < 0.0003). In addition, PLTs suspended in M-sol and subjected to an interruption of agitation had lesser Day 7 CD62+ cells, glucose utilization, and lactate production and greater hypotonic stress response, morphology, swirling, and aggregation response than those suspended in plasma (p

Serum levels of ET-1, M30, and angiopoietins-1 and -2 in HELLP syndrome and preeclampsia compared to controls.

PubMed

Karakus, Savas; Bozoklu Akkar, Ozlem; Yildiz, Caglar; Sancakdar, Enver; Cetin, Meral; Cetin, Ali

2016-02-01

We aimed to compare the serum levels of ET-1, M30, and Angs-1 and -2 in patients with preeclampsia or HELLP syndrome, and normal controls. In this cross-sectional study of 74 pregnant women, serum levels of ET-1, M30, and Angs-1 and -2 were measured in preeclamptic patients with or without HELLP syndrome. 74 pregnant women; 37 had healthy pregnancies, 25 had preeclampsia (PE), and 12 had HELLP syndrome. The age, body mass index, gravidity, and parity of patients with normal pregnancy, PE, and HELLP syndrome were comparable (p > 0.05). In HELLP syndrome, compared to healthy or preeclamptic pregnancies, platelet count was lower (p < 0.05) and the values of hepatic function tests were higher (p < 0.05). In HELLP syndrome, ET-1, M30, and Ang-2 were higher compared to healthy or preeclamptic pregnancies (p < 0.05); however, they increased in preeclamptic pregnancies compared to healthy pregnancies though not significant (p > 0.05). In PE or HELLP syndrome, Ang-1 was higher compared to a healthy pregnancy (p < 0.05); however, in HELLP syndrome, it was also higher than in PE though not significant (p > 0.05). We found no significant correlation among these biomarkers and hematological and biochemical parameters (p > 0.05). For the diagnosis of HELLP syndrome, increased levels of ET-1, M30, and Angs-1 and -2 appear as promising biomarkers after determination of their standardized threshold levels after further studies. As an apoptosis-related biomarker, serum M30 level has a merit to be the most promising test for prediction or differential diagnosis of HELLP syndrome in PE patients.

The silencing effect of miR-30a on ITGA4 gene expression in vitro: an approach for gene therapy.

PubMed

Darzi, Leila; Boshtam, Maryam; Shariati, Laleh; Kouhpayeh, Shirin; Gheibi, Azam; Mirian, Mina; Rahimmanesh, Ilnaz; Khanahmad, Hossein; Tabatabaiefar, Mohammad Amin

2017-12-01

Integrins are adhesion molecules which play crucial roles in cell-cell and cell-extracellular matrix interactions. Very late antigen-4 or α4β1 and lymphocyte Peyer's patch adhesion molecule-1 or α4β7, are key factors in the invasion of tumor cells and metastasis. Based on the previous reports, integrin α4 ( ITGA4 ) is overexpressed in some immune disorders and cancers. Thus, inhibition of ITGA4 could be a therapeutic strategy. In the present study, miR-30a was selected in order to suppress ITGA4 expression. The ITGA4 3' UTR was amplified, cloned in the Z2827-M67-( ITGA4 ) plasmid and named as Z2827-M67/3'UTR. HeLa cells were divided into five groups; (1) untreated without any transfection, (2) mock with Z2827-M67/3'UTR transfection and X-tremeGENE reagent, (3) negative control with Z2827-M67/3'UTR transfection alone, (4) test with miR-30a mimic and Z2827-M67/3'UTR transfection and (5) scramble with miR-30a scramble and Z2827-M67/3'UTR transfection. The MTT assay was performed to evaluate cell survival and cytotoxicity in each group. Real-time RT-PCR was applied for the ITGA4 expression analysis. The findings of this study showed that miR-30a downregulated ITGA4 expression and had no effect on the cell survival. Due to the silencing effect of miR-30a on the ITGA4 gene expression, this agent could be considered as a potential tool for cancer and immune disorders therapy.

The silencing effect of miR-30a on ITGA4 gene expression in vitro: an approach for gene therapy

PubMed Central

Darzi, Leila; Boshtam, Maryam; Shariati, Laleh; Kouhpayeh, Shirin; Gheibi, Azam; Mirian, Mina; Rahimmanesh, Ilnaz; Khanahmad, Hossein; Tabatabaiefar, Mohammad Amin

2017-01-01

Integrins are adhesion molecules which play crucial roles in cell-cell and cell-extracellular matrix interactions. Very late antigen-4 or α4β1 and lymphocyte Peyer’s patch adhesion molecule-1 or α4β7, are key factors in the invasion of tumor cells and metastasis. Based on the previous reports, integrin α4 (ITGA4) is overexpressed in some immune disorders and cancers. Thus, inhibition of ITGA4 could be a therapeutic strategy. In the present study, miR-30a was selected in order to suppress ITGA4 expression. The ITGA4 3' UTR was amplified, cloned in the Z2827-M67-(ITGA4) plasmid and named as Z2827-M67/3'UTR. HeLa cells were divided into five groups; (1) untreated without any transfection, (2) mock with Z2827-M67/3'UTR transfection and X-tremeGENE reagent, (3) negative control with Z2827-M67/3'UTR transfection alone, (4) test with miR-30a mimic and Z2827-M67/3'UTR transfection and (5) scramble with miR-30a scramble and Z2827-M67/3'UTR transfection. The MTT assay was performed to evaluate cell survival and cytotoxicity in each group. Real-time RT-PCR was applied for the ITGA4 expression analysis. The findings of this study showed that miR-30a downregulated ITGA4 expression and had no effect on the cell survival. Due to the silencing effect of miR-30a on the ITGA4 gene expression, this agent could be considered as a potential tool for cancer and immune disorders therapy. PMID:29204174

MASTER-OAFA: Fermi GRB faded optical counterpart detection

NASA Astrophysics Data System (ADS)

Pogrosheva, T.; Lipunov, V.; Podesta, R.; Levato, H.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Gress, O.; Kornilov, V.; Vladimirov, V.; Chazov, V.; Gorbunov, I.; Krylov, A.; Shumkov, V.; Kuvshinov, D.

2017-02-01

During Fermi GBM 508295323 trigger ( GRB_TIME: 2017-02-09 01:08:38.08 UT https://gcn.gsfc.nasa.gov/other/508295323.fermi ) inspection MASTER-OAFA auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered new OT source (Podesta et al. GCN #20650) at (RA, Dec) = 07h 23m 07.30s -52d 14m 46.6s on 2017-02-09 02:07:07.478UT with unfiltered m_OT=17.4 (mlimit=18.1m).

Effects of Sensing Capability on Ground Platform Survivability During Ground Forces Maneuver Operations

DTIC Science & Technology

2014-09-01

Hellfire missiles, Hydra -70mm rockets, and M230 30mm automatic cannon (Boeing 2014). Hellfire missiles have shaped-charge HEAT warheads and are...capable of destroying an MBT. These missiles have an operational range between 500 m to 8,000 m (AeroWeb 2014). The Hydra -70mm rocket is also capable of...platforms, but it lacks precision (Army Technology 2014). Similar to the Hydra -70mm rocket, the M230 30mm cannon is effective against soft skin

Helmet Tracker Requirements and Measurement Verification

DTIC Science & Technology

2007-02-01

D . P . Meador, "A comparison of head-steered and aircraft-fixed infrared...150 180 Azimuth (deg) In du ce d E le va tio n E rr or (m ill iR ad ) -0.30 -0.20 -0.10 0.00 0.10 0.20 0.30 In du ce d A zi m ut h E rr or (m ic...J. Lewandowski, W. Stephens and L. A. Haworth (eds.), 2465, 184-193, SPIE, Bellingham, WA, 1995. 2. E. E. Geiselman, P . R. Havig and M. T.

Comparison of the Bayer VERSANT HCV RNA 3.0 and the Roche COBAS Amplicor HCV Monitor, Version 2.0, assays in HCV genotype 4 infection.

PubMed

Jessner, W; Watkins-Riedel, T; Müller, C; Formann, E; Gschwantler, M; Ferenci, P

2007-11-01

Prediction of treatment response is clinically important in chronic hepatitis C virus (HCV) genotype 4 infection. Early viral kinetics is useful in this respect for genotype 1 but interpretation is dependent on assay linearity and reproducibility. The VERSANT HCV RNA 3.0 (bDNA-3.0) and the COBAS Amplicor HCV Monitor 2.0 (HCM-2.0) have been widely used quantitative assays. We wanted to comparatively evaluate the two tests in a large genotype 4 sample. Genotyping was performed by NS5b sequencing. Viral load was tested in parallel in 32 patients at least six times on antiviral therapy with interferon alpha (IFNalpha). Totally, 198 samples within a quantitative range from undetectable to about 7 x 10(6) IU/mL (bDNA-3.0) were obtained and compared. Twenty-two samples with viral load above 500 000 IU/mL tested by HCM-2.0 were 1:100 diluted and retested. Quantitative values were fitted to a third order polynomial (M = 0.118303 + 1.07503 x V+ 0.0112128 x V(2) - 0.0055504 x V(3); M...HCM-2.0, V...bDNA-3.0, both log IU/mL) showing progressive nonlinearity of HCM-2.0 above 100 000 IU/mL but better clinical sensitivity with respect to bDNA-3.0. Dilution lead to a gain of at least a factor of 2.7 and thus, overestimation compared with bDNA-3.0. Deviation from linearity and overestimation upon dilution by HCM-2.0 are similar with HCV genotype 4, compared with other HCV genotypes. Differences in test performance were not detected for subtypes but for individual patients possibly related to specific quasi-species patterns. The interpretation of viral kinetic data becomes difficult due to overestimation upon dilution of baseline values by HCM-2.0.

Antimutagenic and antioxidant activity of a protein fraction from aerial parts of Urtica dioica.

PubMed

Di Sotto, Antonella; Mazzanti, Gabriela; Savickiene, Nijole; Staršelskytė, Rasa; Baksenskaite, Vaida; Di Giacomo, Silvia; Vitalone, Annabella

2015-06-01

Urtica dioica L. (Urticaceae), stinging nettle, has been employed as a folklore remedy for a wide spectrum of ailments, including urinary disorders, prostatic hyperplasia, and liver diseases. It has been also used traditionally for cancer treatment. To evaluate the potential chemopreventive properties of a protein fraction from the aerial part of Urtica dioica (namely UDHL30). UDHL30 has been tested for the antimutagenic activity in bacteria (50-800 μg/plate; Ames test by the preincubation method) and for the cytotoxicity on human hepatoma HepG2 cells (0.06-2 mg/mL; 24 and 48 h incubation). Moreover, the antioxidant activity of UDHL30 (0.1-1200 μg/mL; ABTS and superoxide-radical scavenger assays) was evaluated as potential protective mechanisms. UDHL30 was not cytotoxic on HepG2 cells up to 2 mg/mL; conversely, it exhibited a strong antimutagenic activity against the mutagen 2-aminoanthracene (2AA) in all strains tested (maximum inhibition of 56, 78, and 61% in TA98, TA100, and WP2uvrA strains, respectively, at 800 μg/plate). In addition, a remarkable scavenging activity against ABTS radical and superoxide anion (IC50 values of 19.9 ± 1.0 μg/mL and 75.3 ± 0.9 μg/mL, respectively) was produced. UDHL30 possesses antimutagenic and radical scavenging properties. Being 2AA a pro-carcinogenic agent, we hypothesize that the antimutagenicity of UDHL30 can be due to the inhibition of CYP450-isoenzymes, involved in the mutagen bioactivation. The radical scavenger ability could contribute to 2AA-antimutagenicity. These data encourage further studies in order to better define the potential usefulness of UDHL30 in chemoprevention.

Predictors of 30-day mortality in patients admitted to ED for acute heart failure.

PubMed

Marchetti, Matthieu; Benedetti, Antoine; Mimoz, Olivier; Lardeur, Jean-Yves; Guenezan, Jérémy; Marjanovic, Nicolas

2017-03-01

Acute heart failure (AHF) is a leading cause of admission in emergency departments (ED). It is associated with significant in-hospital mortality, suggesting that there is room for improvement of care. Our aims were to investigate clinical patterns, biological characteristics and determinants of 30-day mortality. We conducted a single site, retrospective review of adult patients (≥18years) admitted to ED for AHF over a 12-month period. Data collected included demographics, clinical, biological and outcomes data. Epidemiologic data were collected at baseline, and patients were followed up during a 30-day period. There were a total of 322 patients. Mean age was 83.9±9.1years, and 47% of the patients were men. Among them, 59 patients (18.3%) died within 30days of admission to the ED. The following three characteristics were associated with increased mortality: age>85years (OR=1.5[95%CI:0.8-2.7], p=0.01), creatinine clearance <30mL/min (OR=2.6[95%CI:1.4-5], p<0.001) and Nt-proBNP >5000pg/mL (OR=2.2[95%CI:1.2-4], p<0.001). The best Nt-proBNP cut-off value to predict first-day mortality was 9000pg/mL (area under the curve (AUC) [95%CI] of 0.790 [0.634-0.935], p<0.001). For 7-day mortality, it was 7900pg/mL (0.698 [0.578-0.819], p<0.001) and for 30-day mortality, 5000pg/mL (0.667 [0.576-0.758], p<0.001). Nt-proBNP level on admission, age and creatinine clearance, are predictive of 30-day mortality in adult patients admitted to ED for AHF. Copyright © 2016 Elsevier Inc. All rights reserved.

Senescence marker protein 30 (SMP30)/regucalcin (RGN) expression decreases with aging, acute liver injuries and tumors in zebrafish

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fujisawa, Koichi; Terai, Shuji, E-mail: terais@yamaguchi-u.ac.jp; Hirose, Yoshikazu

2011-10-22

Highlights: {yields} Zebrafish SMP30/RGN mRNA expression decreases with aging. {yields} Decreased expression was observed in liver tumors as compared to the surrounding area. {yields} SMP30/RGN is important for liver proliferation and tumorigenesis. -- Abstract: Senescence marker protein 30 (SMP30)/regucalcin (RGN) is known to be related to aging, hepatocyte proliferation and tumorigenesis. However, expression and function of non-mammalian SMP30/RGN is poorly understood. We found that zebrafish SMP30/RGN mRNA expression decreases with aging, partial hepatectomy and thioacetamide-induced acute liver injury. SMP30/RGN expression was also greatly decreased in a zebrafish liver cell line. In addition, we induced liver tumors in adult zebrafish bymore » administering diethylnitrosamine. Decreased expression was observed in foci, hepatocellular carcinomas, cholangiocellular carcinomas and mixed tumors as compared to the surrounding area. We thus showed the importance of SMP30/RGN in liver proliferation and tumorigenesis.« less

Influence of advection on measurements of the net ecosystem-atmosphere exchange of CO2 from a very tall tower

NASA Astrophysics Data System (ADS)

Yi, C.; Davis, K. J.; Bakwin, P. S.; Berger, B. W.; Marr, L. C.

2000-04-01

In most studies of the net ecosystem-atmosphere exchange of CO2 (NEE) using tower-based eddy covariance (EC) systems it has been assumed that advection is negligible. In this study we use a scalar conservation budget method to estimate the contribution of advection to NEE measurements from a very tall tower in northern Wisconsin. We examine data for June-August 1997. Measured NEE0, calculated as the sum of the EC flux plus the rate of change of storage below the EC measurement level, is expected to be constant with measurement height, and we take the differences between levels as a measure of advection. We find that the average difference in total advection ΔFCadtot between 30 and 122 m is as large as 6 μmol m-2s-1 during the morning transition from stable to convective conditions and the average difference ΔFCadtot between 122 and 396 m is as large as 4 μmol m-2s-1 during daytime. For the month of July, advection between 30 and 122 m is 27% of the diurnally integrated NEE0 at 122 m, and advection between 122 and 396 m accounts for 5% of the NEE0 observed at 396 m. The observed differences of advection often have significant correlation with the vertical integral of wind speed within the same layer. This indicates that the horizontal advection contribution to NEE could be significant. Direct observations of the vertical gradient in CO2 show that ΔFCadtot cannot be explained by vertical advection alone. It is hypothesized that differing flux footprints and pooling of CO2 in the heterogeneous landscape causes the advection contribution. The magnitudes of the total advection component FCadtot of NEE at the 30 m level are roughly estimated by a linear extrapolation. A peak in FCadtot at 30 m of ˜ 3 μmol m-2 s-1 during the morning transition is predicted for all three months. The July integrated FCadtot is estimated to be 10% of the diurnally integrated NEE0 at 30 m.

78 FR 12768 - National Institute of Diabetes and Digestive and Kidney Diseases; Notice of Closed Meeting

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... constitute a clearly unwarranted invasion of personal privacy. Name of Committee: National Institute of... (X01): Hepatitis E and Advanced Chronic Hepatitis C. Date: March 27, 2013 Time: 12:30 p.m. to 1:30 p.m...

75 FR 27350 - National Institute of Diabetes and Digestive and Kidney Diseases; Notice of Closed Meeting

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..., Endocrinology and Metabolic Diseases. Date: June 7, 2010. Time: 1:30 p.m. to 4:30 p.m. Agenda: To review and... Assistance Program Nos. 93.847, Diabetes, Endocrinology and Metabolic Research; 93.848, Digestive Diseases...

77 FR 14815 - National Institute of Diabetes and Digestive and Kidney Diseases Notice of Closed Meetings

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... Selected Type 1 Diabetes Clinical Studies (DP3). Date: April 2, 2012. Time: 2:30 p.m. to 5:30 p.m. Agenda...; 93.848, Digestive Diseases and Nutrition Research; 93.849, Kidney Diseases, Urology and Hematology...

75 FR 54092 - North Pacific Fishery Management Council; Public Meeting

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76 FR 34124 - RTCA Program Management Committee

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75 FR 71182 - RTCA Program Management Committee

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... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration RTCA Program Management Committee AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of RTCA Program Management Committee... Management Committee. DATES: The meeting will be held June 10, 2010 from 8:30 a.m. to 1:30 p.m. ADDRESSES...

Salt preference: age and sex related variability.

PubMed

Verma, Punam; Mittal, Sunita; Ghildiyal, Archana; Chaudhary, Lalita; Mahajan, K K

2007-01-01

Salt preference was assessed in 60 adults of 18-21 yrs of age (30 males and 30 females) and in 60 children of 7-12 yrs of age (30 boys and 30 girls). Subjects rated the preference on Likert scale for popcorns of five salt concentrations (OM, 1M, 2M, 3M and +3M). Statistical analysis using Two way ANOVA revealed statistically significant effect of age and sex on salt preference (F4,100 = 15.027, P < 0.01) and One Way ANOVA revealed statistically significant sex difference in salt preference of adults (F4,50 = 16.26, P < 0.01) but no statistically significant sex difference in salt preference of children (F4,50 = 4.08, P > 0.05). Dietary experiences during development and more physical activity in children may be responsible for higher salt preference in children while finding no sex variability in children favours the role of sex hormones in salt preference of male and females.

Physical exertion and immediate mental performance of sixth-grade children.

PubMed

McNaughten, D; Gabbard, C

1993-12-01

The intent of this investigation was to examine the potential influence of varying durations of physical exertion at different times of the day on immediate mathematical performance by 120 sixth-grade boys and girls. Subjects were assigned to two control and two treatment groups (Solomon Four-group Design), with treated subjects administered physical exertion (paced walking at controlled moderate intensity) for durations of 20, 30, and 40 min. at three different times of the school day [8:30 a.m., 11:50 a.m. (before lunch), 2:20 p.m.] over 3 weeks. After each exertion session, subjects were immediately administered a 90-sec. mathematical computation test. Analysis indicated no significant differences in mathematical performance at any duration in the morning, but scores were significantly higher at 11:50 a.m. and 2:20 p.m. at 30- and 40-min. durations in comparison to the 20-min. duration. There were no differences by gender of subject.

IDA/OSD R&M (Institute for Defense Analyses/Office of the Secretary of Defense Reliability and Maintainability) Study Bibliography.

DTIC Science & Technology

1983-08-01

30 -IOLW. c • o:,,tas z ".9’ IM moOm X-272 IDA... 30 -2 • . ,, ... .L.. . .i W . . .’ I".• : V.*.’’. ;, .. . ... ," ... ,. . - 4..; ,,, .,, R&M STUDY,. DOCUMENTS LIST ( by Subject )-.:,’.i AS OF: 22...DE60. Maintainability Standardization Document Program Plan, Revision 1, Draft, DoD, 30 April 1982. DE82. Acquisition and Management of

77 FR 73106 - Self-Regulatory Organizations; NYSE MKT LLC; Notice of Filing and Immediate Effectiveness of...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-07

... Amending Rule 123C(9)(a)(1)(ii)--Equities To Delete the Requirement That the Order Acceptance Cut-Off Time... order acceptance cut-off time cannot be past 4:30 p.m. The text of the proposed rule change is available... acceptance cut-off time cannot be past 4:30 p.m. (or 30 minutes after the scheduled close in the case of an...

Role of the neutral endopeptidase 24.11 in the conversion of big endothelins in guinea-pig lung parenchyma.

PubMed Central

Lebel, N.; D'Orléans-Juste, P.; Fournier, A.; Sirois, P.

1996-01-01

1. We have studied the conversion of big endothelin-1 (big ET-1), big endothelin-2 (big ET-2) and big endothelin-3 (big ET-3) and characterized the enzyme involved in the conversion of the three peptides in guinea-pig lung parenchyma (GPLP). 2. Endothelin-1 (ET-1), endothelin-2 (ET-2) and endothelin-3 (ET-3) (10 nM to 100 nM) caused similar concentration-dependent contractions of strips of GPLP. 3. Big ET-1 and big ET-2 also elicited concentration-dependent contractions of GPLP strips. In contrast, big ET-3, up to a concentration of 100 nM, failed to induce a contraction of the GPLP. 4. Incubation of strips of GPLP with the dual endothelin converting enzyme (ECE) and neutral endopeptidase (NEP) inhibitor, phosphoramidon (10 microM), as well as two other NEP inhibitors thiorphan (10 microM) or SQ 28,603 (10 microM) decreased by 43% (P < 0.05), 42% (P < 0.05) and 40% (P < 0.05) the contractions induced by 30 nM of big ET-1 respectively. Captopril (10 microM), an angiotensin-converting enzyme inhibitor, had no effect on the contractions induced by big ET-1. 5. The incubation of strips of GPLP with phosphoramidon (10 microM), thiorphan (10 microM) or SQ 28,603 (10 microM) also decreased by 74% (P < 0.05), 34% and 50% (P < 0.05) the contractions induced by 30 nM big ET-2 respectively. As for the contractions induced by big ET-1, captopril (10 microM) had no effect on the concentration-dependent contractions induced by big ET-2. 6. Phosphoramidon (10 microM), thiorphan (10 microM) and SQ 28,603 (10 microM) significantly potentiated the contractions of strips of GPLP induced by both ET-1 (30 nM) and ET-3 (30 nM). However, the enzymatic inhibitors did not significantly affect the contractions induced by ET-2 (30 nM) in this tissue. 7. These results suggest that the effects of big ET-1 and big ET-2 result from the conversion to ET-1 and ET-2 by at least one enzyme sensitive to phosphoramidon, thiorphan and SQ 28,603. This enzyme corresponds possibly to EC 3.4.24.11 (NEP 24.11) and could also be responsible for the degradation of ETs in the GPLP. Images Figure 4 PMID:8825361

Impact of nano zero valent iron (NZVI) on methanogenic activity and population dynamics in anaerobic digestion.

PubMed

Yang, Yu; Guo, Jialiang; Hu, Zhiqiang

2013-11-01

Nano zero valent iron (NZVI), although being increasingly used for environmental remediation, has potential negative impact on methanogenesis in anaerobic digestion. In this study, NZVI (average size = 55 ± 11 nm) showed inhibition of methanogenesis due to its disruption of cell integrity. The inhibition was coincident with the fast hydrogen production and accumulation due to NZVI dissolution under anaerobic conditions. At the concentrations of 1 mM and above, NZVI reduced methane production by more than 20%. At the concentration of 30 mM, NZVI led to a significant increase in soluble COD (an indication of cell disruption) and volatile fatty acids in the mixed liquor along with an accumulation of H2, resulting in a reduction of methane production by 69% (±4% [standard deviation]). By adding a specific methanogenesis inhibitor-sodium 2-bromoethanesulfonate (BES) to the anaerobic sludge containing 30 mM NZVI, the amount of H2 produced was only 79% (±1%) of that with heat-killed sludge, indicating the occurrence of bacterially controlled hydrogen utilization processes. Quantitative PCR data was in accordance with the result of methanogenesis inhibition, as the level of methanogenic population (dominated by Methanosaeta) in the presence of 30 mM NZVI decreased significantly compared to that of the control. On the contrary, ZVI powder (average size <212 μm) at the same concentration (30 mM) increased methane production presumably due to hydrogenotrophic methanogenesis of hydrogen gas that was slowly released from the NZVI powder. While it is a known fact that NZVI disrupts cell membranes, which inhibited methanogenesis described herein, the results suggest that the rapid hydrogen production due to NZVI dissolution also contribute to methanogenesis inhibition and lead to bacterially controlled hydrogenotrophic processes. Copyright © 2013 Elsevier Ltd. All rights reserved.

Downscaling 250-m MODIS growing season NDVI based on multiple-date landsat images and data mining approaches

USGS Publications Warehouse

Gu, Yingxin; Wylie, Bruce K.

2015-01-01

The satellite-derived growing season time-integrated Normalized Difference Vegetation Index (GSN) has been used as a proxy for vegetation biomass productivity. The 250-m GSN data estimated from the Moderate Resolution Imaging Spectroradiometer (MODIS) sensors have been used for terrestrial ecosystem modeling and monitoring. High temporal resolution with a wide range of wavelengths make the MODIS land surface products robust and reliable. The long-term 30-m Landsat data provide spatial detailed information for characterizing human-scale processes and have been used for land cover and land change studies. The main goal of this study is to combine 250-m MODIS GSN and 30-m Landsat observations to generate a quality-improved high spatial resolution (30-m) GSN database. A rule-based piecewise regression GSN model based on MODIS and Landsat data was developed. Results show a strong correlation between predicted GSN and actual GSN (r = 0.97, average error = 0.026). The most important Landsat variables in the GSN model are Normalized Difference Vegetation Indices (NDVIs) in May and August. The derived MODIS-Landsat-based 30-m GSN map provides biophysical information for moderate-scale ecological features. This multiple sensor study retains the detailed seasonal dynamic information captured by MODIS and leverages the high-resolution information from Landsat, which will be useful for regional ecosystem studies.

Late-night hours draw busy patients.

PubMed

1999-10-01

Planned Parenthood of Houston and southeast Texas is currently implementing a reproductive health care clinic with night-time hours. The clinic provides for the reproductive health care needs of college students with after-class jobs, women with two jobs or with night-shift employment, and all other women who do not have time to go to a daytime clinic. The clinic operates twice a month on Fridays and was initially open 10:30 p.m. - 7 a.m., but now it has changed its hours to 7:30 p.m. - 3:30 a.m. The clinic is staffed by one clinician and two clinic assistants, which attend to an average of 17-22 patients per night's schedule. Women who use the clinic keep their appointments better and do not have to wait so long for care.

Assessment of Required Accuracy of Digital Elevation Data for Hydrologic Modeling

NASA Technical Reports Server (NTRS)

Kenward, T.; Lettenmaier, D. P.

1997-01-01

The effect of vertical accuracy of Digital Elevation Models (DEMs) on hydrologic models is evaluated by comparing three DEMs and resulting hydrologic model predictions applied to a 7.2 sq km USDA - ARS watershed at Mahantango Creek, PA. The high resolution (5 m) DEM was resempled to a 30 m resolution using method that constrained the spatial structure of the elevations to be comparable with the USGS and SIR-C DEMs. This resulting 30 m DEM was used as the reference product for subsequent comparisons. Spatial fields of directly derived quantities, such as elevation differences, slope, and contributing area, were compared to the reference product, as were hydrologic model output fields derived using each of the three DEMs at the common 30 m spatial resolution.

78 FR 21599 - Defense Acquisition University Board of Visitors; Notice of Federal Advisory Committee Meeting

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2013-04-11

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77 FR 27278 - National Emergency Medical Services Advisory Council (NEMSAC); Notice of Federal Advisory...

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... announces the date, time, and location of the meeting, which will be open to the public. The purpose of... a.m. to 12 p.m. EDT. A public comment period will take place on May 30, 2012 between 3:30 p.m. and 4... given under the Federal Advisory Committee Act, Public Law 92-463, as amended (5 U.S.C. App.). The...

76 FR 41221 - Federal Advisory Committee; Defense Health Board (DHB) Meeting

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2011-07-13

... Federal Advisory Committee Act of 1972 (5 U.S.C., Appendix as amended), the Sunshine in the Government Act of 1976 (5 U.S.C. 552b, as amended), and 41 CFR 102-3.150, and in accordance with Section 10(a)(2) of...:30 p.m. and from 1:30 to 5 p.m. DATES: The meeting will be held-- August 8, 2011 8-9 a.m...

76 FR 58300 - Affirmative Decisions on Petitions for Modification Granted in Whole or in Part

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77 FR 48165 - Center for Scientific Review; Notice of Closed Meeting

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2012-08-13

... Functional Neurobiology. Date: September 5, 2012. Time: 2:30 p.m. to 4:30 p.m. Agenda: To review and evaluate..., Comparative Medicine; 93.333, Clinical Research; 93.306, 93.333, 93.337, 93.393-93.396, 93.837-93.844, 93.846...

75 FR 32239 - Notice of Availability and Notice of Public Hearing for the Environmental Assessment for the...

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MG132 as a proteasome inhibitor induces cell growth inhibition and cell death in A549 lung cancer cells via influencing reactive oxygen species and GSH level.

PubMed

Han, Yong Hwan; Park, Woo Hyun

2010-07-01

Carbobenzoxy-Leu-Leu-leucinal (MG132) as a proteasome inhibitor has been shown to induce apoptotic cell death through formation of reactive oxygen species (ROS). In the present study, we evaluated the effects of MG132 on the growth of A549 lung cancer cells in relation to cell growth, ROS and glutathione (GSH) levels. Treatment with MG132 inhibited the growth of A549 cells with an IC(50) of approximately 20 microM at 24 hours. DNA flow cytometric analysis indicated that 0.5 approximately 30 microM MG132 induced a G1 phase arrest of the cell cycle in A549 cells. Treatment with 10 or 30 microM MG132 also induced apoptosis, as evidenced by sub-G1 cells and annexin V staining cells. This was accompanied by the loss of mitochondrial membrane potential (MMP; Delta psi m). The intracellular ROS levels including O(2) (*-) were strongly increased in 10 or 30 microM MG132-treated A549 cells but were down-regulated in 0.1, 0.5 or 1 microM MG132-treated cells. Furthermore, 10 or 30 microM MG132 increased mitochondrial O(2) (*- ) level but 0.1, 0.5 or 1 microM MG132 decreased that. In addition, 10 or 30 microM MG132 induced GSH depletion in A549 cells. In conclusion, MG132 inhibited the growth of human A549 cells via inducing the cell cycle arrest as well as triggering apoptosis, which was in part correlated with the changes of ROS and GSH levels. Our present data provide important information on the anti-growth mechanisms of MG132 in A549 lung cancer cells in relation to ROS and GSH.

An Experimental Test of Buffer Utility as a Technique for Managing Pool-Breeding Amphibians

PubMed Central

Veysey Powell, Jessica S.; Babbitt, Kimberly J.

2015-01-01

Vegetated buffers are used extensively to manage wetland-dependent wildlife. Despite widespread application, buffer utility has not been experimentally validated for most species. To address this gap, we conducted a six-year, landscape-scale experiment, testing how buffers of different widths affect the demographic structure of two amphibian species at 11 ephemeral pools in a working forest of the northeastern U.S. We randomly assigned each pool to one of three treatments (i.e., reference, 100m buffer, 30m buffer) and clearcut to create buffers. We captured all spotted salamanders and wood frogs breeding in each pool and examined the impacts of treatment and hydroperiod on breeding-population abundance, sex ratio, and recapture rate. The negative effects of clearcutting tended to increase as forest-buffer width decreased and be strongest for salamanders and when other stressors were present (e.g., at short-hydroperiod pools). Recapture rates were reduced in the 30m, but not 100m, treatment. Throughout the experiment for frogs, and during the first year post-cut for salamanders, the predicted mean proportion of recaptured adults in the 30m treatment was only 62% and 40%, respectively, of that in the reference treatment. Frog sex ratio and abundance did not differ across treatments, but salamander sex ratios were increasingly male-biased in both cut treatments. By the final year, there were on average, only about 40% and 65% as many females predicted in the 100m and 30m treatments, respectively, compared to the first year. Breeding salamanders at short-hydroperiod pools were about 10% as abundant in the 100m versus reference treatment. Our study demonstrates that buffers partially mitigate the impacts of habitat disturbance on wetland-dependent amphibians, but buffer width and hydroperiod critically mediate that process. We provide the first experimental evidence showing that 30-m-wide buffers may be insufficient for maintaining resilient breeding populations of pool-dependent amphibians, at least during the first six years post-disturbance. PMID:26196129

Short- or long-rest intervals during repeated-sprint training in soccer?

PubMed

Iaia, F Marcello; Fiorenza, Matteo; Larghi, Luca; Alberti, Giampietro; Millet, Grégoire P; Girard, Olivier

2017-01-01

The present study compared the effects of two repeated-sprint training (RST) programs, differing in duration of the between-sprint rest intervals, on various soccer-related exercise performances. For 5 weeks during the competitive season, twenty-nine young trained male soccer players either replaced two of their habitual fitness conditioning sessions with RST characterized by short (5-15; n = 9) or long (5-30; n = 10) rest intervals, or served as control (n = 10). The 5-15 and 5-30 protocols consisted of 6 repetitions of 30-m (~5 s) straight-line sprints interspersed with 15 s or 30 s of passive recovery, respectively. 5-15 improved 200-m sprint time (2.0±1.5%; p<0.05) and had a likely positive impact on 20-m sprint performance, whereas 5-30 lowered the 20-m sprint time (2.7±1.6%; p<0.05) but was only possibly effective for enhancing the 200-m sprint performance. The distance covered during the Yo-Yo Intermittent Recovery Test Level 2 increased following 5-15 (11.4±5.0%; p<0.05), which was possibly better than the non-significant 6.5% enhancement observed in 5-30. Improvements in the total time of a repeated-sprint ability test were possibly greater following 5-30 (3.6±0.9%; p<0.05) compared to 5-15 (2.6±1.1%; p<0.05). Both RST interventions led to similar beneficial (p<0.05) reductions in the percentage decrement score (~30%) of the repeated-sprint ability test as well as in blood lactate concentration during submaximal exercise (17-18%). No changes occurred in the control group. In soccer players, RST over a 5-week in-season period is an efficient means to simultaneously develop different components of fitness relevant to match performance, with different benefits induced by shorter compared to longer rest intervals.

Short- or long-rest intervals during repeated-sprint training in soccer?

PubMed Central

Iaia, F. Marcello; Fiorenza, Matteo; Larghi, Luca; Alberti, Giampietro; Millet, Grégoire P.; Girard, Olivier

2017-01-01

The present study compared the effects of two repeated-sprint training (RST) programs, differing in duration of the between-sprint rest intervals, on various soccer-related exercise performances. For 5 weeks during the competitive season, twenty-nine young trained male soccer players either replaced two of their habitual fitness conditioning sessions with RST characterized by short (5–15; n = 9) or long (5–30; n = 10) rest intervals, or served as control (n = 10). The 5–15 and 5–30 protocols consisted of 6 repetitions of 30-m (~5 s) straight-line sprints interspersed with 15 s or 30 s of passive recovery, respectively. 5–15 improved 200-m sprint time (2.0±1.5%; p<0.05) and had a likely positive impact on 20-m sprint performance, whereas 5–30 lowered the 20-m sprint time (2.7±1.6%; p<0.05) but was only possibly effective for enhancing the 200-m sprint performance. The distance covered during the Yo-Yo Intermittent Recovery Test Level 2 increased following 5–15 (11.4±5.0%; p<0.05), which was possibly better than the non-significant 6.5% enhancement observed in 5–30. Improvements in the total time of a repeated-sprint ability test were possibly greater following 5–30 (3.6±0.9%; p<0.05) compared to 5–15 (2.6±1.1%; p<0.05). Both RST interventions led to similar beneficial (p<0.05) reductions in the percentage decrement score (~30%) of the repeated-sprint ability test as well as in blood lactate concentration during submaximal exercise (17–18%). No changes occurred in the control group. In soccer players, RST over a 5-week in-season period is an efficient means to simultaneously develop different components of fitness relevant to match performance, with different benefits induced by shorter compared to longer rest intervals. PMID:28199402

Effects of capture-related injury on postcapture movement of white-tailed deer.

PubMed

Dechen Quinn, Amy C; Williams, David M; Porter, William F; Fitzgerald, Scott D; Hynes, Kevin

2014-04-01

Capture-related injuries or deaths of wildlife study subjects pose concerns to researchers, from considerations for animal welfare to inflated project costs and biased data. Capture myopathy (CM) is an injury that can affect an animal's survival ≤ 30 days postrelease, but is often difficult to detect without close monitoring and immediate necropsy. We evaluated the influence of capture and handling on postcapture movement in an attempt to characterize movement rates of animals suffering from CM. We captured and global positioning system-collared 95 white-tailed deer (Odocoileus virginianus) in central and northern New York during 2006-2008. Six juveniles died within 30 days postrelease, and necropsy reports indicated that two suffered CM (2%). We compared postcapture movement rates for juveniles that survived >30 days with those that died ≤ 30 days postcapture. Survivor movement rates (43.74 m/hr, SD = 3.53, n = 28) were significantly higher than rates for deer that died within 30 days (17.70 m/hr, SD = 1.57, n = 6) (P<0.01). Additionally, movement rates of juveniles that died of CM (15.1 m/hr) were 5.1 m/hr lower than those for juveniles that died of other causes ≤ 30 days postcapture (20.2 m/hr), but we were unable to evaluate this statistically because of insufficient sample size. We found no difference in vital rates (temperature, heart rate, respiration rate) during handling between survivors and juveniles that died within 30 days postcapture but observed that survivors were in better body condition at capture. These results suggest that deer likely to die within the 30-day CM window can be identified soon after capture, provided that intensive movement data are collected. Further, even if necropsy reports are unavailable, these animals should be censored from analysis because their behavior is not representative of movements of surviving animals.

Degradation and decoloration of textiles wastewater by electron beam irradiation: Effect of energy, current and absorbed dose

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bakar, Khomsaton Abu; Zulkafli,; Hashim, Siti A'aisah

2014-09-03

In this study, electron beam accelerator (EB) was used to treat textiles wastewater from Rawang Industrial Park, Selangor. The objectives were to determine effective energy, beam current and absorbed dose required for decoloration and degradation of the textiles effluent. The textiles effluent was irradiated in a batch with various energy of 1MeV to 3MeV at constant beam current of 30mA. It was observed that removal of color and COD increases with higher beam energy. The EB energy of 1MeV effectively to removed 58% color and 19% COD. For textile effluent sample irradiated at fix energy of 1MeV and 3Mev butmore » at different beam current 10mA, 20mA and 30mA. It was observed that removal of color and COD increases with the increased of beam current at each energy. However removal of color was significantly better at 1Mev as compared to 3Mev. In the case of textiles effluent, irradiated at doses of 17, 20,25,30, 35, 100 and 200kGy using 30 kW power of EB (1Mev, 30mA), results shows removal of BOD{sub 5}, COD and color were in the range 9%-33%, 14%-38% and 43%-78% respectively.« less

Optimal bearing gap of a multiarc radial bearing in a hydrodynamically levitated centrifugal blood pump for the reduction of hemolysis.

PubMed

Kosaka, Ryo; Yasui, Kazuya; Nishida, Masahiro; Kawaguchi, Yasuo; Maruyama, Osamu; Yamane, Takashi

2014-09-01

We have developed a hydrodynamically levitated centrifugal pump as a bridge-to-decision device. The purpose of the present study is to determine the optimal bearing gap of a multiarc radial bearing in the developed blood pump for the reduction of hemolysis. We prepared eight pump models having bearing gaps of 20, 30, 40, 80, 90, 100, 180, and 250 μm. The driving conditions were set to a pressure head of 200 mm Hg and a flow rate of 4 L/min. First, the orbital radius of the impeller was measured for the evaluation of the impeller stability. Second, the hemolytic property was evaluated in an in vitro hemolysis test. As a result, the orbital radius was not greater than 15 μm when the bearing gap was between 20 and 100 μm. The relative normalized index of hemolysis (NIH) ratios in comparison with BPX-80 were 37.67 (gap: 20 μm), 0.95 (gap: 30 μm), 0.96 (gap: 40 μm), 0.82 (gap: 80 μm), 0.77 (gap: 90 μm), 0.92 (gap: 100 μm), 2.76 (gap: 180 μm), and 2.78 (gap: 250 μm). The hemolysis tended to increase at bearing gaps of greater than 100 μm due to impeller instability. When the bearing gap decreased from 30 to 20 μm, the relative NIH ratios increased significantly from 0.95 to 37.67 times (P < 0.01) due to high shear stress. We confirmed that the optimal bearing gap was determined between 30 and 100 μm in the developed blood pump for the reduction of hemolysis. Copyright © 2014 International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.

Microscopic Study of the Influence of Impurities on Interface Bonding.

DTIC Science & Technology

1985-01-25

with different impurities (P,S) levels were specially made by Armco, Inc. In addition to these four alloys, commercial alloys including Armco’s Nitronic ...25 30 40 50 60 70 Percent Intergranular, % IG Figu re A. 10 Variation of K and plane stress Keff with IGA for the Fe-P-W specimens. 20 AIO 40 Fe-P-Mo...iIS- Ke f f q !m=- 0.218 35 E 30 - 30- ~m=-0. 145 25 20 25 30 40 50 60 70 Percent Intergranular, % IG Figurej A.9 Vriation of K and plane stress Keff

MASTER OT J072007.30+451611.6: Cataclysmic Variable with an Extreme Hot Spot

NASA Astrophysics Data System (ADS)

Denisenko, D.

2018-05-01

MASTER team has reported an unusual object MASTER OT J072007.30+451611.6 in ATel #11620 (T. Pogrosheva et al.). The object was observed at 17.0-17.2m during 9 minutes from 22:15:26 to 22:24:30 UT on 2018-04-26, but was below the detection limit (18.8m) 45 minutes before the first positive observation and 43 minutes after the last one. As noted by S. Otero in AAVSO VSX entry, MASTER OT J072007.30+451611.6 has an X-ray counterpart XMMSL2 J072007.4+451615.

Rapamycin impairs metabolism-secretion coupling in rat pancreatic islets by suppressing carbohydrate metabolism.

PubMed

Shimodahira, Makiko; Fujimoto, Shimpei; Mukai, Eri; Nakamura, Yasuhiko; Nishi, Yuichi; Sasaki, Mayumi; Sato, Yuichi; Sato, Hiroki; Hosokawa, Masaya; Nagashima, Kazuaki; Seino, Yutaka; Inagaki, Nobuya

2010-01-01

Rapamycin, an immunosuppressant used in human transplantation, impairs beta-cell function, but the mechanism is unclear. Chronic (24 h) exposure to rapamycin concentration dependently suppressed 16.7 mM glucose-induced insulin release from islets (1.65+/-0.06, 30 nM rapamycin versus 2.35+/-0.11 ng/islet per 30 min, control, n=30, P<0.01) without affecting insulin and DNA contents. Rapamycin also decreased alpha-ketoisocaproate-induced insulin release, suggesting reduced mitochondrial carbohydrate metabolism. ATP content in the presence of 16.7 mM glucose was significantly reduced in rapamycin-treated islets (13.42+/-0.47, rapamycin versus 16.04+/-0.46 pmol/islet, control, n=30, P<0.01). Glucose oxidation, which indicates the velocity of metabolism in the Krebs cycle, was decreased by rapamycin in the presence of 16.7 mM glucose (30.1+/-2.7, rapamycin versus 42.2+/-3.3 pmol/islet per 90 min, control, n=9, P<0.01). Immunoblotting revealed that the expression of complex I, III, IV, and V was not affected by rapamycin. Mitochondrial ATP production indicated that the respiratory chain downstream of complex II was not affected, but that carbohydrate metabolism in the Krebs cycle was reduced by rapamycin. Analysis of enzymes in the Krebs cycle revealed that activity of alpha-ketoglutarate dehydrogenase (KGDH), which catalyzes one of the slowest reactions in the Krebs cycle, was reduced by rapamycin (10.08+/-0.82, rapamycin versus 13.82+/-0.84 nmol/mg mitochondrial protein per min, control, n=5, P<0.01). Considered together, these findings indicate that rapamycin suppresses high glucose-induced insulin secretion from pancreatic islets by reducing mitochondrial ATP production through suppression of carbohydrate metabolism in the Krebs cycle, together with reduced KGDH activity.

Marginal and internal adaptation of ceramic crown restorations fabricated with CAD/CAM technology and the heat-press technique.

PubMed

Mously, Hisham A; Finkelman, Matthew; Zandparsa, Roya; Hirayama, Hiroshi

2014-08-01

The accuracy of chairside computer-aided design and computer-aided manufacturing (CAD/CAM) restorations is questionable, and the effect of the die spacer settings is not well stated in the literature. The purpose of the study was to evaluate the marginal and internal adaptation of E4D crowns fabricated with different spacer thicknesses and to compare these crowns with those fabricated with the heat-press technique. The E4D system was used to fabricate 30 crowns for the first 3 groups, with different spacer thickness settings: 30 μm, 60 μm, and 100 μm. In the fourth group, 10 lithium disilicate crowns were fabricated with the heat-press technique. The occlusal gap, axial gap, vertical marginal gap, and absolute marginal discrepancy were evaluated by x-ray microtomography. Statistical significance was assessed with the Kruskal-Wallis test (α=.05). For post hoc analyses, the Mann-Whitney U test was used alongside the Bonferroni correction for multiple comparisons (α=.008). Within the CAD/CAM groups, the 30-μm spacer thickness resulted in the lowest median axial gap (90.04 μm), whereas the 60-μm spacer thickness resulted in the lowest median occlusal gap (152.39 μm). The median marginal gap values of the CAD/CAM-60 group (49.35 μm) and CAD/CAM-100 group (46.65 μm) were lower than those of the CAD/CAM-30 group (55.18 μm). No significant differences among the CAD/CAM groups were observed for absolute marginal discrepancy. The heat-press group had significantly different values than those of the CAD/CAM groups. The spacer thickness and fabrication technique affected the adaptation of ceramic crowns. The heat-press group yielded the best marginal and internal crown adaptation results. The 30- or 60-μm spacer settings are recommended for the E4D CAD/CAM system. Copyright © 2014 Editorial Council for the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.

Effect of different ventilation regimens on ewes' milk and Canestrato Pugliese cheese quality in summer.

PubMed

Albenzio, Marzia; Santillo, Antonella; Caroprese, Mariangela; Marino, Rosaria; Centoducati, Pasquale; Sevi, Agostino

2005-11-01

The influence of three different ventilation regimens on air pollution in sheep houses and on the quality of ewe milk and of Canestrato Pugliese cheese was investigated during the summer season. The experimental treatments were low ventilation regimen (VR=35 m3/h per ewe) split in 30-min ventilation cycles (LOV-30); moderate ventilation regimen (VR=70 m3/h per ewe) split in 30-min ventilation cycles (MOV-30); moderate ventilation regimen (VR=70 m3/h per ewe) split in 60-min ventilation cycles (MOV-60). The LOV-30 milk had higher microbial load and bulk milk somatic cell count (BMSCC) and resulted in a weaker casein matrix in the curd compared with the MOV-30 and MOV-60 treatments. At 45 d of ripening, the LOV-30 cheeses had a lower casein content and higher non-casein nitrogen (NCN) and water-soluble nitrogen (WSN) contents than the MOV-30 and MOV-60 cheeses. Urea-polyacrylamide gel electrophoresis (urea-PAGE) of the pH 4.6-soluble N extract showed that the MOV-60 cheeses had fewer bands derived from casein (CN) hydrolysis than the LOV-30 or MOV-30 cheeses, despite its having exhibited the highest plasmin (PL) activity levels. Our results suggest that the ventilation regimen is critical in dairy sheep housing for optimizing the hygienic quality of ewe milk and the proteolytic processes occurring in Canestrato Pugliese cheese during ripening.

Optimizing high-resolution melting analysis for the detection of mutations of GPR30/GPER-1 in breast cancer.

PubMed

Aihara, Masamune; Yamamoto, Shigeru; Nishioka, Hiroko; Inoue, Yutaro; Hamano, Kimikazu; Oka, Masaaki; Mizukami, Yoichi

2012-06-15

G protein-coupled receptor 30/G protein estrogen receptor-1 (GPR30/GPER-1) is a novel membrane receptor for estrogen whose mRNA is expressed at high levels in estrogen-dependent cells such as breast cancer cell lines. However, mutations in GRP30 related to diseases remain unreported. To detect unknown mutations in the GPR30 open reading frame (ORF) quickly, the experimental conditions for high-resolution melting (HRM) analysis were examined for PCR primers, Taq polymerases, saturation DNA binding dyes, Mg(2+) concentration, and normalized temperatures. Nine known SNPs and 13 artificial point mutations within the GPR30 ORF, as well as single nucleotide variants in DNA extracted from subjects with breast cancers were tested under the optimal experimental conditions. The combination of Expand High Fidelity(PLUS) and SYTO9 in the presence of 2.0 mM MgCl(2) produced the best separation in melting curves of mutations in all regions of the GPR30 ORF. Under these experimental conditions, the mutations were clearly detected in both heterozygotes and homozygotes. HRM analysis of GPR30 using genomic DNA from subjects with breast cancers showed a novel single nucleotide variant, 111C>T in GPR30 and 4 known SNPs. The experimental conditions determined in this study for HRM analysis are useful for high throughput assays to detect unknown mutations within the GPR30 ORF. Copyright © 2012 Elsevier B.V. All rights reserved.

[Verification of the eclossion mark on the sagittal otolith of the larvae of Sardinella aurita (Pisces: Clupeidae)].

PubMed

Balza, M A; Marín, B

2000-12-01

The study of otolith in larvae is important to determine fish age and growth, essential parameters in the study and management of fisheries resources. In this study, the formation of the hatching mark in Sardinella aurita was verified on ichthyoplankton samples collected off southern Cubagua island, Venezuela, from May 1998 to January 1999. The embryos were kept alive using a culture system until they hatched and daily a group of 10 to 30 larvae were fixed in 95% ethanol. An image analysis system was used to measure morphometric characteristics of larvae and sagittal otoliths. Following are mean values in newly hatched larvae: otolith hatching mark distance from nucleus 4.78 m (I.C. 0.36 m, p 0.05 n = 30), increase width 1.46 m (I.C. 0.17 microm, p 0.05, n = 30) and diameter 14.28 m (IC 1.11 m, p 0.05, n = 30). The mean standard length of larvae at age 0 was 3.31 mm (I.C. 0.08 mm, p 0.05, n = 200). The identification of the hatching mark allows the exact calculation of the number of rings in larvae from the natural environment.

Wheeled and Tracked Vehicle Endurance Testing

DTIC Science & Technology

2014-10-02

Vehicle (ATV) 10 10 50 30 Fire Trucks - Crash and Rescue, Brush, Structural 49/56/50 22/16/50 - 29/28/0 a Wheeled Combat 30 40 15 15 Roboticb - 30 50... Wheeled Light W-M = Wheeled Medium W-H = Wheeled Heavy LM-TT = Light/Medium Truck H-TT = Heavy Truck Tractor/Trailer M = Motorcycle FT = Fire ...TYPE Final 3. DATES COVERED (From - To) 4. TITLE AND SUBTITLE Test Operations Procedure (TOP) 02-2-506A Wheeled and Tracked Vehicle Endurance

78 FR 73744 - Airworthiness Directives; The Boeing Company Airplanes

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2013-12-09

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77 FR 66874 - Notice of Sunshine Act Meetings

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2013-08-16

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2010-06-28

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2013-12-27

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Development of a NEHRP site classification map of Chiang Mai city, Thailand, based on shear-wave velocity using the MASW technique

NASA Astrophysics Data System (ADS)

Thitimakorn, Thanop

2013-08-01

To account for site amplification and seismic hazard mapping, the shear-wave velocity (Vs) profile to a depth of 30 m (Vs (30)) is an important parameter and can be used to calculate the ground motion for specific site conditions. In this study, the near-surface Vs profiles of soils were collected at 44 sites in Chiang Mai city using the multi-channel analysis of surface-wave technique. The Vs of each tested location was average weighted to Vs (30) based on the National Earthquake Hazards Reduction Program (NEHRP) criteria. The average Vs (30) value of the alluvium soils was about 362 m s-1, which falls between NEHRP site classes C and D. The average Vs (30) values of flood plain, fluvial clay and natural levee soils (at 300, 299 and 311 m s-1, respectively) all equated to NEHRP class D. The colluvial deposits in the north-western part of the city were mainly composed of gravel, coarse sand and rock fragments, and were assigned to class C (average Vs (30) of 412 m s-1). Soils with lower Vs values will experience higher earthquake ground shaking than those of the bedrock. Accordingly the major part of Chiang Mai city may experience substantial ground shaking due to the amplification in the soft soils.