75 FR 429 - Notice of Intent To Prepare an Environmental Impact Statement for the Vantage to Pomona Heights...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-05

...; HAG10-0008] Notice of Intent To Prepare an Environmental Impact Statement for the Vantage to Pomona... Yakima Training Center (YTC) intend to prepare an Environmental Impact Statement (EIS) for the Vantage to.... ADDRESSES: You may submit comments or resource information related to the Vantage to Pomona Heights 230 kV...

19. Photocopy of photograph (Frashers Fotos, Pomona California, 3 July ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

19. Photocopy of photograph (Frashers Fotos, Pomona California, 3 July 1932, original print in possession of National Park Service, Petrified Forest National Park), 'DEDICATION CEREMONIES RIO PUERCO BRIDGE, PETRIFIED FOREST, ARIZONA - JULY 3, 1932.' - Rio Puerco Bridge, Mainline Road, spanning Rio Puerco, Holbrook, Navajo County, AZ

20. Photocopy of photograph (Frashers Fotos, Pomona California, 3 July ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

20. Photocopy of photograph (Frashers Fotos, Pomona California, 3 July 1932, original print in possession of National Park Service, Petrified Forest National Park), 'ST. JOHNS HIGH SCHOOL BAND, DEDICATION RIO PUERCO BRIDGE, PETRIFIED FOREST, ARIZONA - JULY 3, 1932.' - Rio Puerco Bridge, Mainline Road, spanning Rio Puerco, Holbrook, Navajo County, AZ

Studies on equine recurrent uveitis. II: The role of infection with Leptospira interrogans serovar pomona.

PubMed

Halliwell, R E; Brim, T A; Hines, M T; Wolf, D; White, F H

1985-10-01

An enzyme linked immunosorbent assay was developed for the detection of immunoglobulin class specific antibodies to Leptospira interrogans serovar pomona in the serum and aqueous humor of horses. Serum antibody was also assayed by microscopic agglutination tests. Although higher levels of antibody were found in sera from horses with signs of uveitis, the association was not statistically significant. Antibodies to pomona were detected in the aqueous of 12 eyes from the 101 horses sampled at a slaughterhouse, and in most instances, a comparison of the aqueous/serum antibody level with that of the total aqueous/serum IgG level indicated intraocular antibody synthesis. Antibodies were also found in 4 aqueous (or vitreous) samples out of 9 obtained from horses with clinically documented uveitis and the above comparison again indicated intraocular antibody synthesis. The data point to an important role for pomona as an etiology of equine recurrent uveitis but also emphasize that the initiating cause for this disease is often obscure in that association with leptospirosis cannot be shown in many instances.

Clinical, serological and echocardiographic examination of healthy field dogs before and after vaccination with a commercial tetravalent leptospirosis vaccine.

PubMed

Spiri, Andrea M; Rodriguez-Campos, Sabrina; Matos, José M; Glaus, Tony M; Riond, Barbara; Reusch, Claudia E; Hofmann-Lehmann, Regina; Willi, Barbara

2017-05-25

Leptospirosis is a re-emerging bacterial zoonosis caused by spirochetes of the genus Leptospira. Severe disease has been reported in dogs in Europe despite vaccination with bivalent Leptospira vaccines. Recently, a tetravalent canine Leptospira vaccine (Nobivac® L4) was licenced in Europe. The goal of this study was to investigate clinical signs, microscopic agglutination test (MAT) titres, haematology, blood biochemistry, cardiac (c) Troponin I levels and echocardiography before and after vaccination with this tetravalent vaccine. Forty-eight healthy dogs were prospectively enrolled and vaccinated twice, 3-4 weeks apart (T0 and T1). Before vaccination (T0) and 16-31 days after the second vaccination (T2), MAT (n = 48), haematology (n = 48), blood biochemistry (n = 36) and cTroponin I measurements (n = 29) were performed, and MAT was repeated 347-413 days after the second vaccination (T3, n = 44). Echocardiography was performed before the first and second vaccination (T0 and T1, n = 24). Mild and transient clinical signs within 5 days following the first and second vaccination occurred in 23% and 10% of the dogs, respectively. Before the first vaccination (T0), all dogs showed negative MAT titres for the tested serovars except for Canicola (50% with titres 100-400). At T2, positive MAT titres to the serovars Canicola (100%), Australis (89%), Grippotyphosa (86%), Bratislava (60%), Autumnalis (58%), Copenhageni (42%), Pomona (12%), Pyrogenes (8%) and Icterohaemorrhagiae (2%) were found. Median to high titres (≥ 400) were most common to the serovar Canicola (92%) and less common to the serovars Australis (41%), Grippotyphosa (21%), Bratislava (12%), Autumnalis (4%), Pyrogenes (4%) and Pomona (2%). At T3, positive MAT titres (titre range: 100-400) were found in 2-18% of the dogs to serovars of the vaccine serogroups and in 2-18% of the dogs to the non-vaccine serovars Pomona, Autumnalis, Pyrogenes and Ballum. Haematology, blood biochemistry, c

Cross-Sectional Serological Survey for Leptospira spp. in Beef and Dairy Cattle in Two Districts in Uganda.

PubMed

Dreyfus, Anou; Odoch, Terence; Alinaitwe, Lordrick; Rodriguez-Campos, Sabrina; Tsegay, Amanuel; Jaquier, Valentine; Kankya, Clovice

2017-11-21

Seroprevalence of Leptospira spp. in cattle is unknown in Uganda. The aim of this study was to estimate the seroprevalence of L. interrogans Icterohaemorrhagiae, Pomona, L. kirschneri Butembo, Grippotyphosa, L. borgpetersenii Nigeria, Hardjo, Wolfii, and Kenya and an overall seroprevalence in cattle from Kole and Mbale districts. Two hundred-seventy five bovine sera from 130 small holder farms from Kole ( n = 159) and Mbale ( n = 116), collected between January and July 2015, were tested for antibodies against eight Leptospira strains by Microscopic Agglutination Test. A titer of ≥100 was considered seropositive, indicating past exposure. Overall, the seroprevalence was 19.27% (95% CI 14.9-24.5%). Pomona seroprevalence was highest with 9.45% (6.4-13.7%), followed by Kenya 5.09% (2.9-8.6%), Nigeria 4.00% (2.1-7.2%), Wolfii 3.27% (1.6-6.3%), Butembo 1.86% (0.7-4.4%), Hardjo 1.45% (0.5-3.9%), and Icterohaemorragiae and Grippotyphosa with less than 1% positive. Seroprevalence did not differ between districts and gender ( p ≥ 0.05). Seven animals had titers ≥400. Cross-reactions or exposure to ≥1 serovar was measured in 43% of serum samples. Seroprevalence of 19% implies exposure of cattle to leptospires.

First Genome Sequence of Leptospira interrogans Serovar Pomona, Isolated from a Bovine Abortion

PubMed Central

Varni, Vanina; Koval, Ariel; Nagel, Ariel; Ruybal, Paula

2016-01-01

Leptospirosis is a widespread zoonosis and a re-emergent disease of global distribution with major relevance in veterinary production. Here, we report the whole-genome sequence of Leptospira interrogans serovar Pomona strain AKRFB, isolated from a bovine abortion during a leptospirosis outbreak in Argentina. PMID:27198013

Serologic survey for leptospirae in European brown bears (Ursus arctos) in Croatia.

PubMed

Modrić, Z; Huber, D

1993-10-01

From 1981 to 1991, sera of 42 European brown bears (Ursus arctos) from three areas in Croatia were tested for antibodies against 12 Leptospira interrogans serovars: grippotyphosa, sejroe, australis, pomona, canicola, icterohaemorrhagiae, tarassovi, saxkoebing, ballum, bataviae, poi, and hardjo. Diagnostic levels of antibody were found in 17 (40%) of 42 sera. Evidence of exposure to at least one of the serovars was found in seven of 14 free-ranging bears from the Lika region, four of 12 free-ranging bears from the Gorski Kotar region, zero of six orphaned cubs from the Gorski Kotar region, and six of 10 captive bears from the Zagreb Zoo. Based on the antibody titers, we implicated the following serovars: australis in five bears, sejroe in two bears, canicola in one bear, and icterohaemorrhagiae in one bear. There was a strong correlation between serovars implicated by this survey and serovars previously isolated from small mammals in Croatia.

The complete mitochondrial genomes of the Fenton′s wood white, Leptidea morsei, and the lemon emigrant, Catopsilia pomona

PubMed Central

Hao, Juan-Juan; Hao, Jia-Sheng; Sun, Xiao-Yan; Zhang, Lan-Lan; Yang, Qun

2014-01-01

Abstract The complete mitochondrial genomes of Leptidea morsei Fenton (Lepidoptera: Pieridae: Dis-morphiinae) and Catopsilia pomona (F.) (Lepidoptera: Pieridae: Coliadinae) were determined to be 15,122 and 15,142 bp in length, respectively, with that of L . morsei being the smallest among all known butterflies. Both mitogenomes contained 37 genes and an A+T-rich region, with the gene order identical to those of other butterflies, except for the presence of a tRNA-like insertion, tRNA Leu (UUR), in C . pomona . The nucleotide compositions of both genomes were higher in A and T (80.2% for L . morsei and 81.3% for C . pomona ) than C and G; the A+T bias had a significant effect on the codon usage and the amino acid composition. The protein-coding genes utilized the standard mitochondrial start codon ATN, except the COI gene using CGA as the initiation codon, as reported in other butterflies. The intergenic spacer sequence between the tRNA Ser (UCN) and ND1 genes contained the ATACTAA motif. The A+T-rich region harbored a poly-T stretch and a conserved ATAGA motif located at the end of the region. In addition, there was a triplicated 23 bp repeat and a microsatellite-like (TA) 9 (AT) 3 element in the A+T-rich region of the L. morsei mitogenome , while in C . pomona, there was a duplicated 24 bp repeat element and a microsatellite-like (TA) 9 element. The phylogenetic trees of the main butterfly lineages (Hesperiidae, Papilionidae, Pieridae, Nymphalidae, Lycaenidae, and Riodinidae) were reconstructed with maximum likelihood and Bayesian inference methods based on the 13 concatenated nucleotide sequences of protein-coding genes, and both trees showed that the Pieridae family is sister to Lycaenidae. Although this result contradicts the traditional morphologically based views, it agrees with other recent studies based on mitochondrial genomic data. PMID:25368074

Archaeological Inventory and Evaluation at Milford, Melvern and Pomona Lakes, Eastern Kansas

DTIC Science & Technology

1988-01-01

Milford, Melvern and Pomona Lakes, Kansas US Army Corps of Engneers Environmental Systems Analysis , Inc. Kansas City District Cultural Resources... Analysis , Inc. Cultural Resources Division Accesikn For Kansas City, Kansas NTIS CRA&I UTIC TA’R Larry J. Schits’ Editor Principal Investigator i. 1988...PERFORMING ORGANIZATION NAME AND ADDRESS S0. PROGRAM ELEMENT. PROJECT. TASK AREA & WORK UNIT NUMBERS Environmental Systems Analysis , Inc. P.O. Box

Longitudinal serological survey and herd-level risk factors for Leptospira spp. serovars Hardjo-bovis and Pomona on deer farms with sheep and/or beef cattle.

PubMed

Subharat, S; Wilson, Pr; Heuer, C; Collins-Emerson, Jm

2012-07-01

To investigate the seroprevalence of Leptospira spp. serovars Hardjo-bovis and Pomona on deer and mixed deer, sheep and/or beef cattle farms in the lower North Island of New Zealand and to examine associations between putative risk factors for seropositive deer herds. Serological screening was conducted on 19 commercial deer farms, 16 with sheep and/or beef cattle, between August and October each year between 2006 and 2008. No leptospiral vaccination had been conducted on the farms. On each farm every year, serum samples were collected from a random sample of 20 or more rising 2-year-old replacement animals from each species. The microscopic agglutination test (MAT) was used to detect leptospiral antibodies against Leptospira borgpetersenii serovar Hardjo-bovis and Leptospira interrogans serovar Pomona. For both serovars, a titre of ≥1:48 was considered positive and a herd was considered seropositive if >3 of 20 serum samples were positive. Information on potential herd-level risk factors for deer herds being seropositive was obtained from a questionnaire completed by the farm owner or manager. The mean percentage of deer, cattle and sheep herds seropositive for Hardjo-bovis alone over 3 years was 42%, 53% and 54%, respectively, and for serovar Pomona was 7%, 5% and 0%, respectively. Antibodies to both serovars were found in 23%, 16% and 10% of deer, cattle and sheep herds, respectively. At the individual animal level, 228/1,107 (21%) deer, 308/767 (40%) cattle and 369/1,244 (30%) sheep were seropositive for Hardjo-bovis, 102 (9%) deer, 51 (7%) cattle and 23 (2%) sheep were seropositive for Pomona, and 49 (4%) deer, 28 (4%) cattle and 18 (1%) sheep were seropositive for both serovars. Deer herds were more likely to be seropositive for Hardjo-bovis in 2006 than 2008 (p=0.008), when seropositive in the preceding year (p=0.016) and on hilly compared with flat topography (p<0.001). Deer herds were more likely to be seropositive for Pomona when seropositive in the

Seroprevalence of Leptospirosis in Working Dogs.

PubMed

Lau, S F; Wong, J Y; Khor, K H; Roslan, M A; Abdul Rahman, M S; Bejo, S K; Radzi, R; Bahaman, A R

2017-12-01

Working dogs are canine animals that have been trained to assist human beings in carrying out various tasks. They help in guarding property, performing rescues, assisting the visually impaired or physically handicapped, searching for drugs, explosives, and others. Leptospirosis is one of the most widespread zoonotic diseases in the world and a commonly occurring disease of the tropics and subtropics. In Malaysia, all working dogs are normally vaccinated with serovars, Pomona, Icterohaemorrhagiae, Canicola, and Grippotyphosa based on protocols recommended from other countries. The duration of immunity in vaccinated dogs for Leptospira can last up to 13 months; however, there is no full crossprotection between the different serovars. Five representative canine units from different government agencies in Malaysia (n = 96 dogs) were recruited in this study. For detection, the microscopic agglutination test was performed by incubating the serum from dogs with various serovars of leptospires, namely, Icterohaemorrhagiae, Canicola, Pomona, Grippotyphosa, Australis, Bataviae, Javanica, Tarassovi, Hebdomadis, Lai, and Pyrogenes. The plasma obtained was used for polymerase chain reaction (PCR) analysis, for the detection of 16S rRNA, and lipL 32 genes of Leptospira. Out of the 96 dogs sampled, only 3 dogs were positive toward serovars, Australis, Bataviae, and Javanica, based on the cutoff point at 1:80. The seroprevalence of canine leptospirosis in this population was 3.1% (n = 3/96). However, all 96 blood samples of working dogs tested negative for both pathogenic and nonpathogenic Leptospira genes. The results revealed that, by vaccination alone, working dogs were not fully protected against leptospirosis and could pose a risk to dog handlers. A preventative and control protocol for leptospirosis is warranted, and its implementation should be monitored and improved accordingly from time to time, in order to maintain a healthy condition in both working dogs and their

Serological survey on Leptospira infection in slaughtered swine in North-Central Italy.

PubMed

Bertelloni, F; Turchi, B; Vattiata, E; Viola, P; Pardini, S; Cerri, D; Fratini, F

2018-05-30

Swine can act as asymptomatic carriers of some Leptospira serovars. In this study, 1194 sera from 61 farms located in five different Regions of North-West Italy were collected from slaughtered healthy pigs. Presence of antibody against four Leptospira serovars was evaluated. Overall, 52.5% of analysed farms presented at least one positive animal and 34.4% presented at least one positive swine with titre ⩾1:400. A percentage of 16.6% sera was positive and 5.9% samples presented a positive titre ⩾1:400. Tuscany and Lombardy showed the highest percentage of positive farms (64.3% and 54.6%, respectively) and sera (28.5% and 13.3%, respectively), probably due to environmental conditions and potential risk factors, which promote maintenance and spreading of Leptospira in these areas. The main represented serogroups were Australis (21.3% positive farms, 8.2% positive sera) and Pomona (18.0% positive farms, 8.1% positive sera). In swine, these serogroups are the most detected worldwide; however, our results seem to highlight a reemerging of serogroup Pomona in pigs in investigated areas. A low percentage of sera (0.6%) scored positive to Canicola, leaving an open question on the role of pigs in the epidemiology of this serovar. Higher antibody titres were detected for serogroups Australis and Pomona. Swine leptospirosis is probably underestimated in Italy and could represent a potential risk for animal and human health.

Pomona College Dreamers and Achievers Profile

DOE Office of Scientific and Technical Information (OSTI.GOV)

Meyers, C. A.

As an undergraduate at Pomona, Carol Meyers ’00 enjoyed proofs and pure math, but she also had a hankering to solve real-world problems. This eventually led her to discover the field of operations research, in which she earned a PhD from MIT, and from there onto a career at a national laboratory. “National labs are great because they serve a role between academia and industry,” she says, “solving problems too large or too applied for academia, and insufficiently profitdriven for industry.” Her workplace, Lawrence Livermore National Laboratory, is home to the world’s largest laser, frequent home to the world’s fastestmore » supercomputer, and the namesake to element 116 (Livermorium) on the periodic table. During her 10+ years at the laboratory Carol has worked in the areas of energy grid modernization, nuclear counterterrorism, cyber security, stockpile stewardship, and supercomputing, often as a consultant providing mathematical modeling and optimization expertise. Carol has two young kids and serves as co-chair of the New Moms’ Group at her workplace. “Being in a supportive community of moms has really helped me at work,” she says, “because let’s face it – moms know how to get things done!” “People talk a lot about the importance of networking, but this doesn’t have to mean starting technical discussions with strangers. The ability to bond with and relate to other people is far more important, and many women do this very well. Some of my best work networking connections have come through our employer-affiliated daycare.”« less

Serological surveillance of Leptospirosis in Italy: two‑year national data (2010‑2011).

PubMed

Tagliabue, Silvia; Figarolli, Bianca Maria; D'Incau, Mario; Foschi, Giovanni; Gennero, Maria Silvia; Giordani, Roberta; Giordani, Roberta; Natale, Alda; Papa, Paola; Ponti, Nicoletta; Scaltrito, Domenico; Spadari, Luisa; Vesco, Gesualdo; Ruocco, Luigi

2016-06-30

Nowadays, leptospirosis is a re‑emerging widespread infectious disease often underestimate worldwide. The National Reference Centre for Leptospirosis (NRCL), at the Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Brescia (Italy), with the cooperation of all the other Istituti Zooprofilattici Sperimentali (IIZZSS), evaluated the distribution of such important zoonosis in Italy. Serological data obtained between 2010‑2011 by each laboratory were collected by the NRCL and discussed. Serum samples collected from 43,935 animal specimens were analysed by the Microscopic Agglutination Test (MAT), using a panel of 8 serogroups as antigens (Australis, Ballum, Canicola, Grippotyphosa, Icterohaemorrhagiae, Pomona, Sejroe, Tarassovi). A MAT cut‑off of 1:100 was used to identify the serological positivities, 6,279 sera showed positive titers. Bovine (46.9%), swine (27.5%), ovine and goat (7.4%), dog (6.9%), and wild boar (4.5%) samples were delivered to the Laboratories more frequently than equine and other species sera. Data analysis showed that the most common serogroups in Italy are: Australis present in dogs, wild boars, horses, hares, swine, foxes, and rodents; Sejroe detected in cattle, sheep, goats, and buffaloes; Icterohaemorrhagiae present in dogs, goats, and foxes; Pomona detected in swine, cattle, and wild species; Grippotyphosa reported in hares.

Recent Transmission of a Novel Alphacoronavirus, Bat Coronavirus HKU10, from Leschenault's Rousettes to Pomona Leaf-Nosed Bats: First Evidence of Interspecies Transmission of Coronavirus between Bats of Different Suborders

PubMed Central

Lau, Susanna K. P.; Li, Kenneth S. M.; Tsang, Alan K. L.; Shek, Chung-Tong; Wang, Ming; Choi, Garnet K. Y.; Guo, Rongtong; Wong, Beatrice H. L.; Poon, Rosana W. S.; Lam, Carol S. F.; Wang, Sylvia Y. H.; Fan, Rachel Y. Y.; Chan, Kwok-Hung; Zheng, Bo-Jian

2012-01-01

Although coronaviruses are known to infect various animals by adapting to new hosts, interspecies transmission events are still poorly understood. During a surveillance study from 2005 to 2010, a novel alphacoronavirus, BatCoV HKU10, was detected in two very different bat species, Ro-BatCoV HKU10 in Leschenault's rousettes (Rousettus leschenaulti) (fruit bats in the suborder Megachiroptera) in Guangdong and Hi-BatCoV HKU10 in Pomona leaf-nosed bats (Hipposideros pomona) (insectivorous bats in the suborder Microchiroptera) in Hong Kong. Although infected bats appeared to be healthy, Pomona leaf-nosed bats carrying Hi-BatCoV HKU10 had lower body weights than uninfected bats. To investigate possible interspecies transmission between the two bat species, the complete genomes of two Ro-BatCoV HKU10 and six Hi-BatCoV HKU10 strains were sequenced. Genome and phylogenetic analyses showed that Ro-BatCoV HKU10 and Hi-BatCoV HKU10 represented a novel alphacoronavirus species, sharing highly similar genomes except in the genes encoding spike proteins, which had only 60.5% amino acid identities. Evolution of the spike protein was also rapid in Hi-BatCoV HKU10 strains from 2005 to 2006 but stabilized thereafter. Molecular-clock analysis dated the most recent common ancestor of all BatCoV HKU10 strains to 1959 (highest posterior density regions at 95% [HPDs], 1886 to 2002) and that of Hi-BatCoV HKU10 to 1986 (HPDs, 1956 to 2004). The data suggested recent interspecies transmission from Leschenault's rousettes to Pomona leaf-nosed bats in southern China. Notably, the rapid adaptive genetic change in BatCoV HKU10 spike protein by ∼40% amino acid divergence after recent interspecies transmission was even greater than the ∼20% amino acid divergence between spike proteins of severe acute respiratory syndrome-related Rhinolophus bat coronavirus (SARSr-CoV) in bats and civets. This study provided the first evidence for interspecies transmission of coronavirus between bats of

Production of reactive oxygen (H2O2) and nitrogen (NO) intermediates and tnf-α in mice genetically selected for high (H) and low (L) antibody response and experimentally infected with Leptospira serovar pomona

PubMed Central

Haanwinckel, Maria Cristina Santos; de Oliveira, Silvio Luis

2011-01-01

The aim of the present study was to evaluate the activity of macrophages, and the production of TNF-α and antibodies against experimental infection by Leptospira serovar Pomona in mice genetically selected for High (H) or Low (L) humoral immune response. To evaluate macrophagic activity, peritoneal and splenic lavages were performed for determination of oxygen (H2O2) and nitrogen (NO) intermediates. The production of the tumor necrosis factor (TNF-α) was investigated through bioassays in serum and homogenates of splenic and hepatic cells of control and infected animals, as was as specific antibodies production. The immune response against serovar Pomona in those lines, was characterized by high antibody production, especially in later periods of the infectious process, whereas values of bacterial recovery in culture medium were lower. The production of reactives oxygen and nitrogen intermediate, also helped to eliminate Leptospira Pomona in both lines; H2O2 production an important factor in HIV-A, as well as NO production in LIV-A, especially in later post-inoculation periods. The same was detected for TNF-α. Results suggest that such lines could be an important model to investigate the pathogenesis and the immune response of animals against the several Leptospira serovars. PMID:24031688

Evaluation of a recombinant LipL41 antigen of Leptospira interrogans serovar canicola in ELISA for serodiagnosis of bovine leptospirosis.

PubMed

Mariya, R; Chaudhary, Pallab; Kumar, A A; Thangapandian, E; Amutha, R; Srivastava, S K

2006-11-01

The efficacy of a recombinant leptospiral lipoprotein LipL41 as an antigen for conducting enzyme-linked immunosorbent assay (ELISA) for diagnosis of bovine leptospirosis was evaluated. Using known positive and known negative cattle sera the recombinant antigen was found to be highly reactive in the concentration of 100 ng/well. Using a total of 321 field cattle sera the sensitivity of ELISA as compared to microscopic agglutination test (MAT) was calculated to be 100% whereas the specificity was 85.3%. The seropositivity of leptospirosis among bovine population was found to be 21.18% having the predominance of serovars Sejroe and Pomona. It was concluded that rLipL41 protein could be a putative diagnostic candidate for serodiagnosis of bovine leptospirosis.

Seroprevalence of Leptospirosis, Brucellosis, and Q Fever in a Wild Red Deer (Cervus elaphus) Population Kept in a Fenced Reserve in Absence of Contact with Livestock.

PubMed

San-Miguel Ayanz, Jose María; Garcia-Peña, Francisco Javier; García-Lunar, Paula; Ortega-Mora, Luis Miguel; Ruano, María José; Álvarez-García, Gema; Collantes-Fernández, Esther

2017-10-01

Wildlife health is of interest for public and animal health because wild animals have been identified as important sentinels for the surveillance for zoonotic pathogens. This work investigated Brucella spp., Coxiella burnetii, and Leptospira spp. infection seroprevalence in a free-ranging red deer population. The study was conducted in a fenced reserve with controlled hunting activity in central Spain with animals that did not have any contact with livestock. Sampling was performed at two time points before and 5 years after the implementation of new management measures, including a reduction in the red deer population in the reserve. In addition, the presence of Leptospira DNA was tested in placental and fetal samples from seropositive pregnant animals. Antibodies against Brucella and Coxiella were not detected in any sample. The seroprevalence of Leptospira was 9.4% (13/137) in the first sampling for serovars Canicola and Panama. Five years later, the prevalence rose to 38.5% (97/252) with Pomona, the only serovar detected. Animals older than 2 years (50%; 70/140) were more likely to be Pomona seropositive than animals ≤2 years old (25.2%; 27/107; p < 0.001). Leptospira DNA was not detected in any sample tested. In conclusion, wild red deer in this area without contact with livestock seem not to play an important role in Brucella spp. and C. burnetii maintenance. The high seroprevalence of Leptospira spp. serogroup Pomona could indicate a risk for people with narrow contact with these animals, but the carrier status was not assessed. Consequently, it is unknown if red deer would represent a risk for human infection. Considering that wild boar could be the source of infection to red deer, the role of wild boar in the spread of leptospirosis and the risk for human infection should be investigated.

ZTF Undergraduate Astronomy Institute at Caltech and Pomona College

NASA Astrophysics Data System (ADS)

Penprase, Bryan Edward; Bellm, Eric Christopher

2017-01-01

From the new Zwicky Transient Facility (ZTF), an NSF funded project based at Caltech, comes a new initiative for undergraduate research known as the Summer Undergraduate Astronomy Institute. The Institute brings together 15-20 students from across the world for an immersive experience in astronomy techniques before they begin their summer research projects. The students are primarly based at Caltech in their SURF program but also includes a large cohort of students enrolled in research internships at Pomona College in nearby Claremont CA. The program is intended to introduce students to research techniques in astronomy, laboratory and computational technologies, and to observational astronomy. Since many of the students are previously computer science or physics majors with little astronomy experience, this immersive experience has been extremely helpful for enabling students to learn about the terminologies, techniques and technologies of astronomy. The field trips to the Mount Wilson and Palomar telescopes deepen their knowledge and excitement about astronomy. Lectures about astronomical research from Caltech staff scientists and graduate students also provide context for the student research. Perhaps more importantly, the creation of a cohort of like-minded students, and the chance to reflect about careers in astronomy and research, give these students opportunities to consider themselves as future research scientists and help them immensely as they move forward in their careers. We discuss some of the social and intercultural aspects of the experience as well, as our cohorts typically include international students from many countries and several students from under-represented groups in science.

[Diagnostic serology of swine leptospirosis in Mexico 1995-2000].

PubMed

Cisneros Puebla, Miguel Angel; Moles Cervantes, Luis Pedro; Rosas, Dolores Gavaldón; Serranía, Nora Rojas; Torres Barranca, Jorge Isaac

2002-01-01

Results obtained from sample testing of 1970 swines from a number of Mexican farms were analyzed. Such samples had been received in the Leptospira Lab of Universidad Autonoma Metropolitana de Xochimilco from 1995 to 2000. Sera with titers equal to or higher than 1:1000 were considered positive; 39,8% of the animals were seropositive (784) and the most frequent serovarieties were bratislava, 22.5%; icterohaemorrhagiae strain Palo Alto, 14,5%; portland vere strain Sinaloa ACR, 13,8%; icterohaemorrhagiae, 11,1%; grippotyphosa, 8,9%; hardjo strain H89,7.2%; tarassovi,7.1%; panama, 5.8%, pomona and hardjo, 5.1%; wolffi, 3%; shermani, 2.4%; pyrogenes, 1.2%; canicola, 0.8%; hebdomadis, 0,5%. The bratislava serovariety has been reported as the cause of reproductive failure in several countries and it holds the first place in serological studies. Therefore, the present paper provides information for stating that this is one of the most significant serovarieties in Mexico.

USDA regulatory guidelines and practices for veterinary Leptospira vaccine potency testing.

PubMed

Srinivas, G B; Walker, A; Rippke, B

2013-09-01

Batch-release potency testing of leptospiral vaccines licensed by the United States Department of Agriculture (USDA) historically was conducted through animal vaccination-challenge models. The hamster vaccination-challenge assay was Codified in 1974 for bacterins containing Leptospira pomona, Leptospira icterohaemorrhagiae, and Leptospira canicola, and in 1975 for bacterins containing Leptospira grippotyphosa. In brief, 10 hamsters are vaccinated with a specified dilution of bacterin. After a holding period, the vaccinated hamsters, as well as nonvaccinated controls, are challenged with virulent Leptospira and observed for mortality. Eighty percent of vaccinated hamsters must survive in the face of a valid challenge. The high cost of the Codified tests, in terms of monetary expense and animal welfare, prompted the Center for Veterinary Biologics (CVB) to develop ELISA alternatives for them. Potency tests for other serogroups, such as Leptospira hardjo-bovis, that do not have Codified requirements for potency testing continue to be examined on a case-by-case basis. Published by Elsevier Ltd.

Serological investigation of Leptospira infection and its circulation in one intensive-type water buffalo farm in the Philippines.

PubMed

Villanueva, Marvin A; Mingala, Claro N; Gloriani, Nina G; Yanagihara, Yasutake; Isoda, Norikazu; Nakajima, Chie; Suzuki, Yasuhiko; Koizumi, Nobuo

2016-02-01

Water buffalo is an indispensable livestock in the Philippines. Leptospirosis is a serious zoonosis that can be fatal to humans and cause reproductive problems in livestock. Leptospirosis has been reported in some countries where water buffaloes are commercially raised, highlighting the Leptospira prevalence in this farming system, but information on leptospirosis in water buffalo farms in the Philippines is limited. In this study, we collected blood samples from rats (n = 21), and water buffaloes (n = 170) from different groups and locations in one intensive-type buffalo farm in the Philippines. Serum was analyzed by microscopic agglutination test (MAT). Anti-Leptospira antibodies reacting with serogroups Canicola, Icterohaemorrhagiae and Pomona were found in sera of 30% tested rats, and 48% of water buffalo sera tested positive for at least one Leptospira strain, in which serogroups Mini, Hebdomadis, Tarassovi and Pyrogenes were predominantly agglutinated. The number of seropositive young water buffaloes (< 1 year-old) was lower than that of older seropositive ones. Furthermore, sera from younger water buffaloes were reactive with single serotypes with low MAT titers, but older animals were reactive with multiple Leptospira strains with variable MAT titers. In addition, antibodies against serogroups Icterohaemorrhagiae and Pomona were detected in both animals. Finally, Leptospira infection was found associated with age and animal grouping, highlighting the impact of management in the persistence of leptospirosis at intensive-type buffalo farm settings in the Philippines. Further investigation and appropriate control strategies are required to prevent leptospirosis from causing risks to public health and economic losses to the water buffalo farming industry.

Passive surveillance of Leptospira infection in swine in Germany.

PubMed

Strutzberg-Minder, Katrin; Tschentscher, Astrid; Beyerbach, Martin; Homuth, Matthias; Kreienbrock, Lothar

2018-01-01

As no current data are available on the prevalence of leptospiral infection in swine in Germany, we analysed laboratory data from diagnostic examinations carried out on samples from swine all over Germany from January 2011 to September 2016. A total of 29,829 swine sera were tested by microscopic agglutination test (MAT) for antibodies against strains of eleven Leptospira serovars. Overall, 20.2% (6025) of the total sample collection tested positive for leptospiral infection. Seropositivity ranged between 16.3% (964) in 2011 and 30.9% (941) in 2016 (January to September only). Of all samples, 11.6% (57.3% of the positives) reacted with only one Leptospira serovar, and only 8.6% (42.7% of the positives) reacted simultaneously with two or more serovars. The most frequently detected serovar was Bratislava, which was found in 11.6% (3448) of all samples, followed by the serovars Australis in 7.3% (2185), Icterohaemorrhagiae in 4.0% (1191), Copenhageni in 4.0% (1182), Autumnalis in 3.7% (1054), Canicola in 2.0% (585), and Pomona in 1.2% (368). Modelling shows that both the year and the reason for testing at the laboratory had statistically strong effects on the test results; however, no interactions were determined between those factors. The results support the suggestion that the seropositivities found may be considered to indicate the state of leptospiral infections in the German swine population. Although data from passive surveillance are prone to selection bias, stratified analysis by initial reason for examination and analyses by model approaches may correct for biases. A prevalence of about 20% for a leptospiral infection is most probable for sows with reproductive problems in Germany, with an increasing trend. Swine in Germany are probably a reservoir host for serovar Bratislava, but in contrast to other studies not for Pomona and Tarassovi.

A serological survey of leptospirosis in cattle of rural communities in the province of KwaZulu-Natal, South Africa.

PubMed

Hesterberg, U W; Bagnall, R; Bosch, B; Perrett, K; Horner, R; Gummow, B

2009-03-01

A serological survey of leptospirosis in cattle originating from rural communities of the province of KwaZulu-Natal (KZN) in South Africa was carried out between March 2001 and December 2003. The survey was designed as a 2-stage survey, using the local diptank as the primary sampling point. In total, 2021 animals from 379 diptanks in 33 magisterial districts were sampled and tested with the microscopic agglutination test (MAT). The apparent prevalence at district level was adjusted for clustering and diagnostic test sensitivity and specificity and displayed in maps. The prevalence of leptospirosis in cattle originating from communal grazing areas of KZN was found to be 19.4% with a 95% confidence interval of 14.8-24.1%. At district level the prevalence of leptospirosis varied from 0 to 63% of cattle. Bovine leptospirosis was found to occur in communal grazing areas throughout the province with the exception of 2 districts. The southeastern regions showed a higher prevalence than other areas of the province; while in some of the northern and western districts a lower prevalence was noted. Several serovars were detected by the MAT and although Leptospira interrogans serovar pomona occurred most frequently, serovars tarrasovi, bratislava, hardjo, canicola and icterohaemorrhagica were also frequently identified. The findings of the survey are discussed.

Disease Surveillance of California Ground Squirrels ( Spermophilus beecheyi ) in a Drive-through Zoo in Oregon, USA.

PubMed

Beest, Julia Ter; Cushing, Andrew; McClean, Modesto; Hsu, Wendy; Bildfell, Robert

2017-07-01

Rodents and other small wild mammals are often considered to be pests and vectors for disease in zoos that house small populations of valuable threatened and endangered animals. In 2005, three nonhuman primates at a drive-through zoo in Oregon, US, acquired tularemia from an unknown source. Due to an abundance of California ground squirrels ( Spermophilus beecheyi ) on zoo grounds, we instituted serosurveillance of this species from July through September 2008 to determine the prevalence of antibodies against pathogens considered to be potentially transmissible to collection animals. Serologic testing was performed for Francisella tularensis ; Leptospira interrogans serovars Canicola, Grippotyphosa, Hardjo, Icterohemorrhagiae, and Pomona; Toxoplasma gondii ; and Yersinia pestis . All squirrels were seronegative for Yersinia pestis (0%; 0/45) and Toxoplasma gondii (0%; 0/20); there was a prevalence of 2% (1/45) for Francisella tularensis antibodies and 57% (24/42) were positive for various Leptospira serovars. Although it remains unclear whether ground squirrels present a significant risk for transmission of disease to zoo animals, vaccination of high-risk zoo animals against leptospirosis warrants consideration. Beyond this, continued vigilance and persistence with various forms of pest control may reduce the likelihood of disease transmission from wildlife hosts to animals in human care.

Opportunities and strategies to further reduce animal use for Leptospira vaccine potency testing.

PubMed

Walker, A; Srinivas, G B

2013-09-01

Hamsters are routinely infected with virulent Leptospira for two purposes in the regulation of biologics: the performance of Codified potency tests and maintenance of challenge culture for the Codified potency tests. Options for reducing animal use in these processes were explored in a plenary lecture at the "International Workshop on Alternative Methods for Leptospira Vaccine Potency Testing: State of the Science and the Way Forward" held at the Center for Veterinary Biologics in September 2012. The use of validated in vitro potency assays such as those developed by the U.S. Department of Agriculture for Leptospira (L.) canicola, Leptospira grippotyphosa, Leptospira pomona, and Leptospira icterohaemorrhagiae rather than the Codified hamster vaccination-challenge assay was encouraged. Alternatives such as reduced animal numbers in the hamster vaccination-challenge testing were considered for problematic situations. Specifically, the merits of sharing challenge controls, reducing group sizes, and eliminating animals for concurrent challenge dose titration were assessed. Options for maintaining virulent, stable cultures without serial passage through hamsters or with decreased hamster use were also discussed. The maintenance of virulent Leptospira without the use of live animals is especially difficult since a reliable means to maintain virulence after multiple in vitro passages has not yet been identified. Published by Elsevier Ltd.

Longitudinal study on the seroprevalence of avian influenza, leptospirosis, and tularemia in an urban population of raccoons (Procyon lotor) in Ontario, Canada.

PubMed

Jardine, Claire; Lindsay, L Robbin; Nicholson, Vivian M; Ojkic, Davor; Prescott, John F

2011-01-01

Raccoons (Procyon lotor) live at high densities, often in close association with people, in urban areas in Ontario and have been implicated as potential reservoirs of numerous zoonotic disease agents. We collected 137 blood samples from 61 apparently healthy raccoons in a small area of Toronto, Ontario, from June to October 2007 as part of a longitudinal study to determine the seasonal patterns of seroprevalence of Francisella tularensis, avian influenza, and Leptospira. In addition, we collected 35 urine samples by cystocentesis from 23 animals to look for evidence of urinary shedding of Leptospira. All samples were serologically negative for F. tularensis and avian influenza. Nineteen of 61 animals (31%) were positive for Leptospira antibodies in one or more trapping periods. The seroprevalence of Leptospira increased from 5% in June to 38% in October. Of the 19 positive animals, 14 were seropositive for serogroup Grippotyphosa, 4 for serogroup Pomona, and 1 for both serogroups Australis and Grippotyphosa. Raccoons were seronegative to serovars representative of serogroups Autumnalis, Canicola, Icterohaemorrhagiae, and Sejroe. Only one urine sample was culture positive for Leptospira (2.9%). Although we found evidence that raccoons in this study were exposed to leptospires belonging to serogroup Grippotyphosa, likely serovar Grippotyphosa, during the summer and able to shed leptospires in urine, further work is required to determine the importance of raccoons as reservoirs of Leptospira in Ontario.

Prevalence of antibodies to Leptospira in wild mammals trapped on livestock farms in Ontario, Canada.

PubMed

Allen, Samantha E; Ojkic, Davor; Jardine, Claire M

2014-07-01

To determine the prevalence and diversity of Leptospira serogroups circulating in wildlife on farms in Ontario, we tested samples from 51 raccoons (Procyon lotor), seven skunks (Mephitis mephitis), four rats (Rattus norvegicus), and three opossums (Didelphis virginiana) that were trapped on 27 livestock (swine [Sus scrofa], cattle [Bos taurus]) farms in 2010. Seventeen of 51 raccoons (33%; 95% confidence interval [CI], 21-48%) sampled were positive for at least one Leptospira serogroup using the microscopic agglutination test. None of the other 14 animals had detectable Leptospira antibodies. On swine farms, 13 of 30 raccoons (43%; 95% CI, 27-61%) were antibody positive, and on cattle farms, four of 21 raccoons (19%; 95% CI, 8-40%) were positive. Leptospira antibody prevalence in raccoons did not differ between swine and cattle farms. Raccoons were positive to serovars representative of serogroups Grippotyphosa, Australis, Icterohaemorrhagiae, and Pomona and were negative to serovars of serogroups Autumnalis, Canicola, and Sejroe. The prevalence of Leptospira antibodies in raccoons in this study is similar to what has been reported previously; however, the diversity of serogroups was higher in this study than what has been reported in raccoons from an urban area of Ontario, Canada. Understanding the prevalence and distribution of Leptospira serogroups in wildlife in Ontario, Canada, is important for the development and maintenance of appropriate disease management strategies in humans, livestock, and companion animals.

78 FR 756 - Notice of Availability of the Draft Environmental Impact Statement for the Proposed Vantage...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-04

... Vantage-Pomona Heights 230kV Transmission Line Project in Yakima, Grant, Benton, and Kittitas Counties, WA...) has prepared a Draft Environmental Impact Statement (EIS) for the Vantage- Pomona Heights 230kV... submit comments related to the Vantage-Pomona Heights 230kV Transmission Line Project by any of the...

Interspecies dynamics among bacteria associated with canine periodontal disease.

PubMed

Sanguansermsri, P; Nobbs, A H; Jenkinson, H F; Surarit, R

2018-02-01

The etiology and pathogenic mechanisms associated with canine periodontal disease are less well understood than the disease in humans. In this study we have reconstructed defined consortia biofilms in vitro of microorganisms identified as prevalent in a same-breed cohort of dogs with or without periodontal disease. Frederiksenia canicola and Neisseria canis were selected as potential early colonizers of salivary pellicle, and Fusobacterium nucleatum and Porphyromonas gulae were included as high incidence canine oral bacteria. N. canis formed a biofilm substratum under aerobic conditions, but was unable to tolerate anaerobic conditions. Fr. canicola exhibited synergistic biofilm growth with Po. gulae under anaerobic conditions, but displayed an antagonistic relationship with Fu. nucleatum. However, strong co-adhesion between Fu. nucleatum and Po. gulae was able to overcome the inhibitory effects of Fr. canicola to facilitate three-species biofilm formation. Parvimonas micra, an anaerobic, asaccharolytic Gram-positive coccus found only under disease conditions in vivo, was able to form biofilms in conjunction with Fr. canicola and Po. gulae. Furthermore, the specific proteolytic activities of biofilms containing Fr. canicola and Po. gulae or Fu. nucleatum and Po. gulae were increased several-fold upon the addition of Pa. micra. This suggests that anaerobic cocci such as Pa. micra might provide a catalyst for progressive tissue destruction, inflammation and alveolar bone loss in canine periodontal disease, in keeping with the keystone-pathogen hypothesis. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

DNA Vaccination of the American Crow (Corvus brachyrhynchos) Provides Partial Protection Against Lethal Challenge with West Nile Virus

DTIC Science & Technology

2007-01-01

mortality but did not provide sterile immunity. RESUMEN. La vacunación del cuervo Americano (Corvus brachyrhynchos) con vacuna de ADN proporciona...casi 100% fatal en el cuervo Americano (Corvus brachyrhynchos). Evaluamos cuatro formulaciones de vacunas en cuervos Americanos, incluyendo una vacuna de...ADN, una vacuna de ADN con adyuvante, ambas aplicadas por la vı́a intramuscular, una vacuna de ADN microencapsulada aplicada por la vı́a oral, y una

Health assessment of wild lowland tapir (Tapirus terrestris) populations in the Atlantic Forest and Pantanal biomes, Brazil (1996-2012).

PubMed

Medici, Emília Patrícia; Mangini, Paulo Rogerio; Fernandes-Santos, Renata Carolina

2014-10-01

Abstract The lowland tapir (Tapirus terrestris) is found in South America and is listed as Vulnerable to Extinction by the International Union for Conservation of Nature, Red List of Threatened Species. Health issues, particularly infectious diseases, are potential threats for the species. Health information from 65 wild tapirs from two Brazilian biomes, Atlantic Forest (AF) and Pantanal (PA), were collected during a long-term study (1996-2012). The study included physic, hematologic and biochemical evaluations, microbiologic cultures, urinalysis, and serologic analyses for antibodies against 13 infectious agents (viral and bacterial). The AF and PA tapirs were significantly different for several hematologic and biochemical parameters. Ten bacteria taxa were identified in the AF and 26 in the PA. Antibodies against five viruses were detected: Bluetongue virus, eastern equine encephalitis virus, western equine encephalitis virus, infectious bovine rhinotracheitis virus, and porcine parvovirus. A high prevalence of exposure to Leptospira interrogans (10 serovars: Autumnalis, Bratislava, Canicola, Copenhageni, Grippotyphosa, Hardjo, Hebdomadis, Icterohaemorrhagiae, Pomona, and Pyrogenes) was detected in both the AF and PA sites. A greater diversity of serovars and higher antibody titers were found in the PA. Statistically significant differences between sites were found for L. interrogans, equine encephalitis virus, and porcine parvovirus. Based on physical evaluations, both AF and PA populations were healthy. The differences in the overall health profile of the AF and PA tapir populations appear to be associated with environmental factors and infectious diseases ecology. The extensive datasets on hematology, biochemistry, urinalysis, and microbiology results from this paper can be used as reference values for wild tapirs.

Assessment of exposure to Leptospira serovars in veterinary staff and dog owners in contact with infected dogs.

PubMed

Barmettler, Reto; Schweighauser, Ariane; Bigler, Susanne; Grooters, Amy M; Francey, Thierry

2011-01-15

To assess patterns of seroreactivity to Leptospira serovars in veterinary professional staff and dog owners exposed to dogs with acute leptospirosis and to contrast these patterns in people with those observed in dogs. Cross-sectional study. Human subjects consisted of 91 people (50 veterinarians, 19 technical staff, 9 administrative personnel, and 13 dog owners) exposed to dogs with leptospirosis. Canine subjects consisted of 52 dogs with naturally occurring leptospirosis admitted to the University of Bern Vetsuisse Faculty Small Animal Clinic in 2007 and 2008. People were tested for seroreactivity to regionally prevalent Leptospira serovars by use of a complement fixation test. A questionnaire designed to identify risk factors associated with seropositivity was used to collect demographic information from each study participant. Dogs were tested for seroreactivity to Leptospira serovars by use of a microscopic agglutination test. On the basis of microscopic agglutination test results, infected dogs were seropositive for antibodies against Leptospira serovars as follows (in descending order): Bratislava (43/52 [83%]), Australis (43/52 [83%]), Grippotyphosa (18/52 [35%]), Pomona (12/52 [23%]), Autumnalis (6/52 [12%]), Icterohemorrhagiae (4/52 [8%]), Tarassovi (2/52 [4%]), and Canicola (1/52 [2%]). All 91 people were seronegative for antibodies against Leptospira serovars. Therefore, statistical evaluation of risk factors and comparison of patterns of seroreactivity to Leptospira serovars between human and canine subjects were limited to theoretical risks. Seroreactivity to Leptospira serovars among veterinary staff adhering to standard hygiene protocols and pet owners exposed to dogs with acute leptospirosis was uncommon.

Fifteen-month duration of immunity for the serovar Grippotyphosa fraction of a tetravalent canine leptospirosis vaccine.

PubMed

Grosenbaugh, Deborah A; Pardo, Maria Camila

2018-06-09

Forty-four specific pathogen-free beagles, median age 65 days, received two subcutaneous doses of either a commercially available, five-way combination vaccine or the same vaccine in combination with a tetravalent Leptospira bacterin (Canicola, Grippotyphosa, Icterohaemorrhagiae, Pomona). They were subsequently challenged with a pathogenic strain L kirschneri serovar Grippotyphosa 470 days following completion of the vaccination protocol. Titres of agglutinating serum antibodies were determined at various time points before and after both vaccination and challenge, along with postchallenge reisolation of the challenge organisms from blood and urine, and evaluation of renal histopathology. Clinical signs of generalised leptospirosis were not observed in any of the dogs after challenge. In order to demonstrate efficacy, leptospirosis was defined as having at least one positive urine sample and a positive renal histopathology score; or, in the absence of renal pathology, multiple positive urine samples. Leptospiremia was not demonstrated in any of the vaccinated dogs versus 27 per cent of the controls; leptospiruria was noted in 5 per cent of the vaccinates compared with 76 per cent of controls; and renal lesions were observed in 15 per cent of the vaccinates and 65 per cent controls. Using these criteria, the vaccine was able to significantly prevent leptospirosis (P=0.0001) in the vaccinated animals. This study establishes duration of immunity of at least 15 months for the prevention of disease and renal excretion of leptospires for the Leptospira serovar Grippotyphosa fraction of a quadrivalent Leptospira vaccine. © British Veterinary Association (unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

Leptospirosis in animals and human contacts in Egypt: broad range surveillance.

PubMed

Samir, Ahmed; Soliman, Rafik; El-Hariri, Mahmoud; Abdel-Moein, Khaled; Hatem, Mahmoud Essam

2015-01-01

Leptospirosis is a re-emerging zoonotic disease of humans and animals worldwide. The disease is caused by pathogenic species of the genus Leptospira. These organisms are maintained in nature via chronic renal infection of carrier animals, which excrete the organisms in their urine. Humans become infected through direct or indirect exposure to infected animals and their urine or through contact with contaminated water and soil. This study was conducted to investigate Leptospira infections as a re-emerging zoonosis that has been neglected in Egypt. Samples from 1,250 animals (270 rats, 168 dogs, 625 cows, 26 buffaloes, 99 sheep, 14 horses, 26 donkeys and 22 camels), 175 human contacts and 45 water sources were collected from different governorates in Egypt. The samples were collected from different body sites and prepared for culture, PCR and the microscopic agglutination test (MAT). The isolation rates of Leptospira serovars were 6.9%, 11.3% and 1.1% for rats, dogs and cows, respectively, whereas the PCR results revealed respective detection rates of 24%, 11.3% and 1.1% for rats, dogs and cows. Neither the other examined animal species nor humans yielded positive results via these two techniques. Only six Leptospira serovars (Icterohaemorrhagiae, Pomona, Canicola, Grippotyphosa, Celledoni and Pyrogenes) could be isolated from rats, dogs and cows. Moreover, the seroprevalence of leptospiral antibodies among the examined humans determined using MAT was 49.7%. The obtained results revealed that rats, dogs and cows were the most important animal reservoirs for leptospirosis in Egypt, and the high seroprevalence among human contacts highlights the public health implications of this neglected zoonosis.

Leptospirosis in an urban setting: cases diagnosed at a private medical center of Western Colombia, 2008-2012.

PubMed

Ramírez-Ramírez, María M; León-Castañeda, Olga M; Rodriguez-Morales, Alfonso J

2015-01-01

Leptospirosis has reemerged as significant threat particularly in developing countries, including those in Latin America. Data from Colombia is still limited and there are no published studies in the Western area of the country. Data on suspected cases were collected over the study period (2008- 2012). Cases were diagnosed clinically and confirmed by ELISA IgG and microscopic agglutination test (MAT) (titers ≥1:400). During the study period 264 suspected cases of leptospirosis were found. From those, 8.33% (22 cases) were microbiologically confirmed. Number of suspected cases increased in the period from 20 (2008) (40 cases/100,000 consultations) to 58 (2012) (120 cases/100,000 consultations). Regard sex distribution, 62.5% were males, 14% in the age group 21-30 y-old, from confirmed cases 95% live in urban areas of Pereira, 25.7% own dogs and 13.2% cats, 32.3% reporting rats at home as well 22.7% at work places. From confirmed cases 72.7% were hospitalized. Clinical findings found were: fever (60.2%), myalgias (47%), and headache (41.9%), among others. All the cases corresponded to Leptospira interrogans. Regard the serovars, in these patients 6 were identified: Australis (54.5%), Icterohaemorrhagiae (45.5%), Canicola (45.5%), Panama (45.5%), Pomona (36.3) and Grippotyphosa (1%). Thirty nine percent of the patients received antimicrobial therapy, 50% ceftriaxone. No deaths occurred. Leptospirosis is an emerging infectious disease that has changed from an occupational disease of veterinarians, farmers, butchers, and other animal handlers to a cause of epidemics in poor and decayed urban communities in developing countries, including those in Latin America such as Colombia.

Brief Report: Applying an Indicator Set to Survey the Health of People with Intellectual Disabilities in Europe

ERIC Educational Resources Information Center

Walsh, Patricia Noonan

2008-01-01

This report gives an account of applying a health survey tool by the "Pomona" Group that earlier documented the process of developing a set of health indicators for people with intellectual disabilities in Europe. The "Pomona" health indicator set mirrors the much larger set of health indicators prepared by the European…

[Detection of pathogenic leptospira in the waste water and sewage sludge of large pig breeding sites].

PubMed

Mînzat, R M; Tomescu, V

1975-01-01

Sewage effluent and sludge from the purification plant of 8 large piggeries was examined for the presence of pathogenic leptospires. By using the methods of Appelman and Van Thiel it was found that 43.1% of samples of effluent were contaminated with L. pomona and O. tarassovi. Altogether 33 strains of pomona and three mixed cultures of pomona and tarassovi were obtained. The isolated strains were shown to be pathogenic by experimental infection of guinea-pigs, rabbits and pregnant and non-pregnant sows. The average period of survival of pathogenic leptospires in sewage effluent was 24 to 48 hours, with a maximum of 96 hours. Leptospires were killed within 24 hours in decanted sludge, owing to its strong acidity.

INFECTIOUS DISEASE AND TOXICOLOGICAL MONITORING OF STRANDED PACIFIC HARBOR SEALS (PHOCA VITULINA RICHARDSI) IN COOK INLET AS SURROGATES FOR MONITORING ENDANGERED BELUGAS (DELPHINAPTERUS LEUCAS).

PubMed

Bauer, Kendra L; Goertz, Caroline E C; Belovarac, Jane A; Walton, Robert W; Dunn, J Lawrence; Tuomi, Pamela

2016-09-01

  Pacific harbor seals ( Phoca vitulina richardsi) and belugas ( Delphinapterus leucas ) eat many of the same prey species, occupy the same geographic area, and demonstrate site fidelity in Cook Inlet, Alaska. Although most direct research involving the critically endangered belugas is currently prohibited, studying harbor seals may provide important information about this beluga population. In recent years, harbor seal populations in Alaska have declined for unknown reasons. As part of its stranding program, the Alaska SeaLife Center (ASLC) managed 59 cases of live and dead stranded harbor seals from Cook Inlet between 1997 and 2011. Animals were screened for a variety of diseases and contaminants of concern. Animals were negative by serology to the following diseases: avian influenza, canine distemper virus, dolphin morbillivirus, porpoise morbillivirus, Leptospira canicola, L. grippotyphosa, L. pomona, Neospora caninum , Sarcocystis neurona , and Toxoplasma gondii . Positive titers were found against Brucella spp., phocine distemper virus, seal herpesvirus-1, L. bratislava, L. hardjo, and L. icterohemorrhagiae. All titers were stable or declining except in one animal with an increasing titer for seal herpesvirus-1. Fecal pathogen screenings identified normal flora as well as stable or declining low levels of potentially pathogenic and opportunistic bacteria, though most were of little concern for seal health. In most animals, toxicology screening showed that the majority of tested contaminants were below detectable limits. The level of evidence of exposure to pathogens of concern was low in harbor seals. Although the infectious disease burden and contaminant levels in belugas in Cook Inlet cannot be definitively determined without direct testing, pathogen and contaminant exposure is expected to be similar to that found in harbor seals in this region, as the harbor seals and belugas share the habitat and food resources.

Correlation of Bedrock Type with the Geography of Leptospirosis

PubMed Central

Kingscote, Barbara F.

1970-01-01

Leptospirosis occurs enzootically over most of Southern Ontario. Leptospira pomona is the serotype most commonly found in outbreaks. Antibodies to L. pomona occur frequently in the sera of deer in wilderness areas. The geographic location of leptospirosis presents a pattern which closely parallels the distribution of Paleozoic bedrock. By contrast, L. pomona infection is absent from areas underlain by Precambrian bedrock. Comparisons of water chemistry, soil type, habitat, and host and pathogen availability in these two geologically distinct environments have not defined the mechanisms involved in the disease pattern. Leptospires resembling saphophytic strains occur widely, regardless of bedrock type. High titers to L. biflexa, a saprophytic serotype, were found frequently in deer sera from a Precambrian area which was surveyed intensively. Antibodies to L. hardjo and L. sejroe occur in many bovine sera from a predominantly Precambrian area where Paleozoic outliers are numerous. Colloidal clay is common to leptospiral habitats. A microenvironment structured by the surface activity of clay is likely to be a key ecological factor in the landscape epizootiology of leptospirosis. In Ontario, bedrock composed of limestone and dolomite formed in the Paleozoic era appears to be a reliable ecological marker for Leptospira pomona infection. PMID:4246001

Cross-protection between experimental anti-leptospirosis bacterins

PubMed Central

Dib, Cristina Corsi; Gonçales, Amane Paldês; de Morais, Zenaide Maria; de Souza, Gisele Oliveira; Miraglia, Fabiana; Abreu, Patricia Antonia Estima; Vasconcellos, Silvio Arruda

2014-01-01

We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with two strains of Leptospira serogroup Pomona: Fromm strain of serovar Kennewicky, isolated from pigs in the United States, and strain GR6 of serovar Pomona isolated from pigs in Brazil. Both were added of aluminum hydroxide as an adjuvant. Experimental bacterins were tested with the hamster potency test in order to assess protection provided against the disease and against the establishment of kidney infection. Controls were polyvalent commercial vaccine produced with Leptospira strains isolated outside Brazil, which included a representative of Pomona serovar, or Sorensen solution added of aluminum hydroxide adjuvant. The challenge was performed with cross-strains of serogroup Pomona tested in accordance with international standards established for the potency test. After 21 days of the challenge, survivors were killed to evaluate the condition of Leptospira renal carrier. Experimental bacterins protected hamsters against homologous and heterologous strains, demonstrating the existence of cross-protection. The commercial vaccine protected the hamsters challenged with both strains, but there was a high proportion of animals diagnosed as renal carriers when the challenge was performed with strain GR6, isolated from pigs in Brazil. PMID:25477946

Diagnosis and seroprevalence of leptospirosis in California sea lions from coastal California.

PubMed

Colagross-Schouten, Angela M; Mazet, Jonna A K; Gulland, Frances M D; Miller, Melissa A; Hietala, Sharon

2002-01-01

The sensitivity and specificity of the microscopic agglutination test (MAT) as a method for detection of exposure to Leptospira spp. in California sea lions (Zalophus californianus) were determined. Sera came from individuals that demonstrated clinical signs of renal disease, had lesions suggestive of leptospirosis at necropsy, and had visible leptospires in silver stained kidney sections as positive controls. Sera from unexposed captive individuals were used as negative controls. The test was 100% sensitive at 1:3,200 for confirming renal infection and 100% specific at negative < 1:100 for detection of Leptospira interrogans scrovar pomona antibodies by MAT in California sea lions. Leptospira interrogans serovar pomona was used as a screening serovar because it has been isolated previously from the kidneys and placentas of California sea lions, and there appears to be cross-reactivity between serovar pomona and other serovars. Sera from 225 free-ranging California sea lions presented to one of three participating California (USA) coastal marine mammal rehabilitation centers in 1996 were then evaluated for antibodies to serovar pomona using the MAT. The overall seroprevalence was 38.2% (86/225), although the prevalence varied among locations from 100% (38/38) in animals at the Marine Mammal Care Center (Fort MacArthur, California, USA) to 0% (0/14) at SeaWorld California (San Diego, California). At The Marine Mammal Center (Sausalito, California) [prevalence 27.8% (48/173)], the majority of seropositive animals were subadults and adults, and males were 4.7 times more likely to be seropositive to serovar pomona than females. When combining results from all three centers, subadult and adult animals were more likely to be seropositive than pups and juvenile sea lions, and the highest proportion of seropositive animals presented during the autumn months. Serum elevations of blood urea nitrogen, creatinine, phosphorus, and/or calcium were associated with seropositivity

Immunologic Control of Diarrheal Disease Due to Enterotoxigenic Escherichia coli

DTIC Science & Technology

1984-01-01

Classical Enteropathogenic (Serotyped) Escherichia coli Strains of Proven Pathogenicity. Infect. Immun. 38:798-801, 1982. 8. Levine, M.M. Vacunas Contra...Microbiol., 18:808-815, 1983. 8 15. Levine, M.M., Lanata, C. Progresos en Vacunas Contra Diarrea Bacteriana. Adelantos Microbiol. Enferm. Inf., 2:67-117

[New considerations on the health of the persons with intellectual developmental disorders].

PubMed

Folch-Mas, Anabel; Cortés-Ruiz, María José; Salvador-Carulla, Luis; Kazah-Soneyra, Natalia; Irazábal-Giménez, Marcia; Muñoz-Lorente, Silvia; Tamarit-Cuadrado, Javier; Martínez-Leal, Rafael

2017-01-01

Recent literature indicates that people with Disorders of Intellectual Development (DID) experience health disparities in the pathologies that they present, and a worst access to health care. However, current evidence-based knowledge is still sparse outside the Anglo-Saxon countries. The POMONA-I and POMONA-II European projects aimed to collect information on the health status of people with DID in Europe. The POMONA-ESP project in Spain is meant to collect health information in a wide and representative sample of persons with DID. Also, there are studies that claim for the need of specialized services for people with DID at the public health system. There are also studies about the current state of the education and training about DID for students within the health sector. In this paper we review the latest evidences about the health of the persons with DID and we present the main research activities and care initiatives about this issue.

Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil

PubMed Central

Miraglia, Fabiana; Moreno, Andréa Mike; Gomes, Cleise Ribeiro; Paixão, Renata; Liuson, Esequiel; Morais, Zenaide Maria; Maiorka, Paulo; Seixas, Fabiana Kömmling; Dellagostin, Odir Antonio; Vasconcellos, Silvio Arruda

2008-01-01

With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts) and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers ≥ 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing, hyalines cylinders and hemorrhagic focuses, hepatic and uterine horns congestion were histological lesions observed in higher frequency in animals positive for leptospira. The silver impregnation (Warthin Starry) confirmed the presence of spirochetes in renal tubules of four females with positive leptospira cultures from kidneys. The serogroup of the five isolates was identified as Pomona by cross agglutination with reference polyclonal antibodies. Molecular characterization of the isolates was carried out by variable-number tandem-repeats analysis. All the isolates revealed a pattern distinct from the L. interrogans Pomona type strain, but identical to a previously identified pattern from strains isolated in Argentina belonging to serovar Pomona. PMID:24031254

Consensus on updating immunizations in patients with primary immunodeficiencies

PubMed

2018-04-01

Las inmunodeficiencias primarias (IDP) constituyen un grupo de enfermedades hereditarias que afectan el número y/o la función de los distintos componentes del sistema inmune. Su prevalencia es de 1:1000-2000 nacimientos. Comprenden defectos de la inmunidad adaptativa, defectos de la inmunidad innata, inmunodeficiencias con fenotipos característicos, trastornos de la regulación inmune, síndromes autoinflamatorios, defectos de los fagocitos y del sistema del complemento y defectos considerados fenocopias de IDP. La vacunación con vacunas inactivadas es segura y puede ser efectiva en muchas inmunodeficiencias; las vacunas vivas atenuadas pueden no ser protectoras en ciertas IDP o presentarse como enfermedad vacunal asociada a la inmunización, lo que conlleva una alta morbimortalidad. Con el objetivo de actualizar las recomendaciones de vacunas en pacientes con IDP, el Comité Nacional de Infectología y el Grupo de Trabajo de Inmunología trabajaron sobre las vacunas que podían indicarse a estos pacientes, convivientes y el equipo de salud.

Contribution of Leptospira, Neospora caninum and bovine viral diarrhea virus to fetal loss of beef cattle in New Zealand.

PubMed

Sanhueza, J M; Heuer, C; West, D

2013-10-01

The profitability of beef breeding farms in New Zealand depends principally on optimal reproductive performance. The aim of this study was to estimate the impact of four major pathogens, bovine viral diarrhea virus (BVDV), Neospora caninum (N. caninum), Leptospira borgpetersenii serovar Hardjo (Hardjo), and Leptospira interrogans serovar Pomona (Pomona), on rates of fetal loss in commercial beef breeding herds. Farms reporting fetal loss were recruited, and a blood sample from aborting cows (cases) was collected. Controls were normally calving cows from the same farm. At least four controls were selected from each farm contributing cases. Samples were tested using ELISA for detection of antibodies against BVDV and N. caninum, and microscopic agglutination test (MAT) for detection of antibody against Hardjo and Pomona. A selection of titer cut-offs was conducted to evaluate the relationship between fetal loss and seropositivity to each pathogen using conditional logistic regression. The cut-off titer with the strongest association with fetal loss was included in the multivariate model. A significant increased risk of fetal loss was found for animals seropositive to N. caninum (odds ratio (OR)=3.36; 95% confidence interval (95% CI)=1.27-8.89), Hardjo (OR=1.84; 95% CI=1.01-3.33), and Pomona in non-vaccinated cows (OR=14.91, 95% CI=1.73-128.84) at the ELISA titer ≥ 30, and MAT titers of ≥ 1:384 and ≥ 1:768 for a positive sample, respectively. A marginally non-significant increased risk of fetal loss was found for animals exposed to BVDV (OR=2.01; 95% CI=0.99-4.11) at the ELISA titer of ≤ 1. Vaccination did not affect ORs for Hardjo or BVDV and no herd vaccinated against N. caninum. Approximately 14.0% of all fetal loss in the beef breeding cattle population in New Zealand may be attributable to BVDV (3.5%), N. caninum (3.0%), Hardjo (4.7%), and Pomona (3.6%). Copyright © 2013 Elsevier B.V. All rights reserved.

Changes in epidemiology of leptospirosis in 2003–2004, a two El Niño Southern Oscillation period, Guadeloupe archipelago, French West Indies

PubMed Central

STORCK, C. HERRMANN; POSTIC, D.; LAMAURY, I.; PEREZ, J. M.

2008-01-01

SUMMARY Our study aimed at analysing the changes in epidemiological features of leptospirosis cases from the hospital of Pointe à Pitre in Guadeloupe in 2003–2004 compared to reliable data in 1994–2001. Leptospirosis incidence increased fourfold during 2002–2004, a period with two El Niño events. Whereas the main risk factors were unchanged (male gender, occupational exposure, contact with cattle or pigs) a major role of rodent exposure emerged (52%, P=0·02, multivariate analysis). Interestingly, mean age of cases shifted to the older population (51·7 years vs. 43 years, P<0·05). Moreover, the Ballum serogroup rose dramatically (36% of incidence) competing with the Icterohaemorragiae serogroup (62%). However, severe forms were less recorded. Our data suggest that the changes in leptospirosis features could be related to exceptional meteorological events and their consequences on rodent populations. We propose the monitoring of rodent population and climatic data as a tool of management of leptospirosis in Guadeloupe. PMID:18096102

76 FR 28000 - Procurement List Proposed Additions and Deletions

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-13

... Contracting Activity: GSA/FSS Household and Industrial Furniture, Arlington, VA. Coverage: A-List for the... Development of Growth & Educational Services, Inc., Pomona, CA. Contracting Activity: General Services...

[Detection of leptospirosis infection in certain wild and domestic animals in Mongolia].

PubMed

Anan'ina, Iu V; Korenberg, E I; Tserennorov, D; Savel'eva, O V; Batjav, D; Otgonbaatar, D; Enkhbold, N; Tsend, E; Erdenechimeg, B

2011-01-01

Serological examination for leptospirosis of domestic and certain species of wild animals in Mongolia. Collection of material from domestic and wild animals was performed in 2009--2010 in 7 aimags (regions) of Eastern, Central and Southern Mongolia. Serological study of filter paper dried blood samples obtained from 51 specimens of cattle and small cattle, camels, and 545 specimens of rodents of various species was performed in microagglutination reaction (MAR) of leptospirae with 13 reference strains. There is a presence in certain regions of Mongolia of anthropurgic loci of leptospirosis infection including arid zones where ecological conditions do not favor the development of epizootic process. The results of the study indicate the epizootic significance of Tarassovi serogroup leptospirae in cattle and Sejroe serogroup (probably hardjo serovar) in goats, sheep and camels. Results of serological studies of desert and steppe specimens of wild fauna of Mongolia suggest a possibility of circulation of leptospirae in natural foci. Detection in a significant percent of cases in tarbagan and long tailed ground squirrel blood sera of agglutinins to Pomona (mozdok) leptospirae with negative MAR results for Pomona (pomona) strain suggests a presence of a pathogen of a previously unknown serovar. However final conclusion could be made only after the isolation of cultures of the pathogen and their identification.

A new tetravalent canine leptospirosis vaccine provides at least 12 months immunity against infection.

PubMed

Klaasen, H L B M; van der Veen, M; Sutton, D; Molkenboer, M J C H

2014-03-15

A key success factor in the vaccination of dogs against leptospirosis is long term protection against establishment of the renal carrier state, in order to protect other dogs, as well as humans, against this re-emerging zoonotic disease. In this paper, we describe the ability of a new European tetravalent vaccine containing antigen from Leptospira interrogans (sensu lato) serogroups Icterohaemorrhagiae, Canicola, Grippotyphosa and Australis to control infection and renal excretion in dogs at 12 months after vaccination. In order to demonstrate the efficacy of all four vaccine components, four separate challenge studies were performed. For each study two groups of dogs were used (a group receiving the leptospirosis vaccine and a control group). Twelve months after the second vaccination all dogs in the vaccine and control groups were challenged, both intraperitoneally and conjunctivally, using a pathogenic challenge strain from one of four serogroups. Parameters recorded post-challenge were: clinical signs of disease, change in body temperature, total leucocyte count, thrombocyte count, presence of challenge organisms in blood, urine and kidney tissue, and evidence of interstitial nephritis at necropsy four weeks after challenge. The vaccine was able to either prevent or significantly reduce infection following challenge with the strains of all four serogroups. The vaccine was also able to prevent or significantly reduce renal infection following Canicola and Icterohaemorrhagiae challenge, and there was a trend of reduction of renal infection with Australis (serovar Bratislava). In the case of the Grippotyphosa study, challenge led to no detectable renal infection in any dog of the control group. In conclusion, in this study significant protective immunity was achieved in dogs 12 months after a basic vaccination schedule of two doses against strains of serogroups Canicola, Icterohaemorrhagiae, Grippotyphosa and Australis. Copyright © 2013 The Authors. Published by

Genotypes of pathogenic Leptospira spp isolated from rodents in Argentina

PubMed Central

Loffler, Sylvia Grune; Pavan, Maria Elisa; Vanasco, Bibiana; Samartino, Luis; Suarez, Olga; Auteri, Carmelo; Romero, Graciela; Brihuega, Bibiana

2014-01-01

Leptospirosis is the most widespread zoonosis in the world and significant efforts have been made to determine and classify pathogenic Leptospira strains. This zoonosis is maintained in nature through chronic renal infections of carrier animals, with rodents and other small mammals serving as the most important reservoirs. Additionally, domestic animals, such as livestock and dogs, are significant sources of human infection. In this study, a multiple-locus variable-number tandem repeat analysis (MLVA) was applied to genotype 22 pathogenic Leptospira strains isolated from urban and periurban rodent populations from different regions of Argentina. Three MLVA profiles were identified in strains belonging to the species Leptospira interrogans (serovars Icterohaemorrhagiae and Canicola); one profile was observed in serovar Icterohaemorrhagiae and two MLVA profiles were observed in isolates of serovars Canicola and Portlandvere. All strains belonging to Leptospira borgpetersenii serovar Castellonis exhibited the same MLVA profile. Four different genotypes were isolated from urban populations of rodents, including both mice and rats and two different genotypes were isolated from periurban populations. PMID:24676656

Serological survey for diseases in free-ranging coyotes (Canis latrans) in Yellowstone National Park, Wyoming.

PubMed

Gese, E M; Schultz, R D; Johnson, M R; Williams, E S; Crabtree, R L; Ruff, R L

1997-01-01

From October 1989 to June 1993, we captured and sampled 110 coyotes (Canis latrans) for various diseases in Yellowstone National Park, Wyoming (USA). Prevalence of antibodies against canine parvovirus (CPV) was 100% for adults (> 24 months old), 100% for yearlings (12 to 24 months old), and 100% for old pups (4 to 12 months old); 0% of the young pups (< 3 months old) had antibodies against CPV. Presence of antibodies against canine distemper virus (CDV) was associated with the age of the coyote, with 88%, 54%, 23%, and 0% prevalence among adults, yearlings, old pups, and young pups, respectively. Prevalence of CDV antibodies declined over time from 100% in 1989 to 33% in 1992. The prevalence of canine infectious hepatitis (ICH) virus antibodies was 97%, 82%, 54%, and 33%, for adults, yearlings, old pups, and young pups, respectively. The percentage of coyotes with ICH virus antibodies also declined over time from a high of 100% in 1989 to 31% in 1992, and 42% in 1993. Prevalence of antibodies against Yersinia pestis was 86%, 33%, 80%, and 7%, for adults, yearlings, old pups, and young pups, respectively, and changed over time from 57% in 1991 to 0% in 1993. The prevalence of antibodies against Francisella tularensis was 21%, 17%, 10%, and 20%, for adults, yearlings, old pups, and young pups, respectively. No coyotes had serologic evidence of exposure to brucellosis, either Brucella abortus or Brucella canis. No coyotes were seropositive to Leptospira interrogans (serovars canicola, hardjo, and icterohemorrhagiae). Prevalence of antibodies against L. interrogans serovar pomona was 7%, 0%, 0%, and 9%, for adults, yearlings, old pups, and young pups, respectively. Antibodies against L. interrogans serovar grippotyphosa were present in 17% of adults and 0% of yearlings, old pups, and young pups. Many infectious canine pathogens (CPV, CDV, ICH virus) are prevalent in coyotes in Yellowstone National Park, with CPV influencing coyote pup survival during the first 3 months

6. Historic American Buildings Survey Nathaniel R. Ewan, Photographer April ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. Historic American Buildings Survey Nathaniel R. Ewan, Photographer April 10, 1936 INTERIOR - STONE SUPPORT IN BASEMENT FOR FIRST FLOOR FIREPLACE - Pomona Hall, Park Boulevard & Euclid Avenue, Camden, Camden County, NJ

Leptospirosis in three workers on a dairy farm with unvaccinated cattle.

PubMed

Benschop, Jackie; Collins-Emerson, Julie; Maskill, Allie; O'Connor, Patrick; Tunbridge, Margaret; Yupiana, Yuni; Weston, Jenny

2017-09-22

We report a one-health investigation of three cases of leptospirosis on a dairy farm with unvaccinated cattle in New Zealand. The cases are discussed in the context of diagnostics, risk factors, persistence of symptoms and outbreak mitigation measures. Clinical and laboratory records from the human cases were reviewed and serological and molecular investigations were conducted into the Leptospira status of cattle and pigs on the farm. Cases presented early in their illness and all three were confirmed within seven days of onset of symptoms by urine PCR and within 18 days by convalescent MAT (two Hardjo, one Pomona). Cattle and pigs had serological evidence of recent infection with Hardjo/Pomona and Pomona/Copenhageni respectively. Pigs were slaughtered and cattle were vaccinated. Post-exposure prophylaxis was given to staff in-contact with the milking herd until the herd had antibiotic treatment at drying-off (approximately four months after the initial case). The utility of PCR testing for Leptospira DNA as both an early and rapid test for leptospirosis was demonstrated. Two of three cases reported persistence of symptoms at least six months after the acute episode and one of these remains unable to work. Risk mitigation measures such as post-exposure prophylaxis, animal vaccination, heightened clinical suspicion of leptospirosis and recognition of context specific risk factors (eg, effluent spreading) demonstrate the value of medical and veterinary experts working together.

RoboSimian Exits Vehicle

NASA Image and Video Library

2015-06-09

JPL's RoboSimian exits its vehicle following a brief drive through a slalom course at the DARPA Robotics Challenge in Pomona, California. This image was taken June 6, 2015. http://photojournal.jpl.nasa.gov/catalog/PIA19324

RoboSimian Driving

NASA Image and Video Library

2015-06-09

JPL's RoboSimian drives a four-wheeled vehicle through a slalom course at the DARPA Robotics Challenge Finals in Pomona, California. This image was taken on June 6, 2015. http://photojournal.jpl.nasa.gov/catalog/PIA19325

7 CFR Appendix B to Part 3434 - List of HSACU institutions, 2012-2013.

Code of Federal Regulations, 2014 CFR

2014-01-01

... OF FOOD AND AGRICULTURE HISPANIC-SERVING AGRICULTURAL COLLEGES AND UNIVERSITIES CERTIFICATION PROCESS... Bakersfield College California State Polytechnic University-Pomona California State University-Bakersfield... Beach California State University-Monterey Bay California State University-San Bernardino College of the...

Mammalian Toxicological Evaluations of TNT Wastewaters. Volume III. Acute and Subacute Mammalian Toxicity of Condensate Water.

DTIC Science & Technology

1979-04-01

Dellen Labs., Inc., Omaha, Nebr- 68134 I The Distemper and Hepatitis fractions are a modified live virus with a canine tissue culture origin. The...Leptospirosis fraction contains physically inactivated Leptospira Canicola and Icterohemorrhagiae Bacterin. DISTEMPER Modified Live Virus-Chick Tissue...ISTEMER-HEPATITIS-LEPTOSPI ROS IS DELCINE HL produced by Del len Labs., Inc., Omaha, Nebraska 68134 The Distemper and Hepatitis fractions are a modified

State Route 60 automated truck facility.

DOT National Transportation Integrated Search

2008-01-01

A research was conducted to evaluate a dedicated automated truck lane along a case study route 60 (Pomona : Freeway) to accommodate higher truck volume using AHS technologies and to estimate the associated costs of : such technologies. A cost analysi...

Foothill Transit Battery Electric Bus Demonstration Results : Second Report

DOT National Transportation Integrated Search

2017-06-01

This report summarizes results of a battery electric bus (BEB) evaluation at Foothill Transit, located in the San Gabriel and Pomona Valley region of Los Angeles County, California. Foothill Transit began a demonstration of three Proterra BEBs in Oct...

Foothill Transit Battery Electric Bus Demonstration Results

DOT National Transportation Integrated Search

2016-01-01

In October 2010, Foothill Transit began a demonstration of three Proterra battery electric buses (BEBs) in its service area located in the San Gabriel and Pomona Valley region of Los Angeles County, California. The agency had a goal of evaluating the...

21 CFR 522.1660a - Oxytetracycline solution, 200 milligrams/milliliter.

Code of Federal Regulations, 2014 CFR

2014-04-01

..., or intravenously for treatment of pneumonia and shipping fever complex associated with Pasteurella... retreatment of calves and yearlings for bacterial pneumonia is impractical, for treatment of infectious bovine.... coli, pneumonia caused by Pasteurella multocida, and leptospirosis caused by Leptospira pomona. (C) 9...

Online Prelectures: An Alternative to Textbook Reading Assignments

NASA Astrophysics Data System (ADS)

Sadaghiani, Homeyra R.

2012-05-01

To engage students in a more meaningful discussion of course material and prompt their higher thinking skills, most instructors expect students to read the course textbook for initial exposure to the course content before class. However, as many instructors are aware, most students do not read their textbook throughout the quarter.1,2 At California State Polytechnic University, Pomona (Cal Poly Pomona) we have adopted web-based multimedia learning modules (MLMs) as prelecture assignments to help students to prepare for the class activities. The MLMs place lecture contents into the hands and control of the learners; similar to "flipped"3 or "inverted"4 classroom approaches, this method allows students to receive key course content outside of class and apply and analyze the content actively during class. In addition to initial exposure to basic principle, the MLMs provide additional worked examples that cannot be thoroughly covered in class.

Transmission and Control of Leptospiral Infection in a Beef Cattle Herd in Southern Alberta

PubMed Central

Kingscote, Barbara

1986-01-01

Leptospiral antibodies were detected in unvaccinated cattle on a 17 000 hectare ranch in the arid southeast region of Alberta. Antibody to serovar hardjo was present before the breeding season in 7% of 42 yearling bulls, 86% of 29 two year old bulls and 5% of 519 cows. Pomona antibody was confined to 3.7% of the cows. Bulls were treated once with dihydrostreptomycin, 25 mg/kg. Bulls and cows were vaccinated twice at a six week interval, with pomona-hardjo-gripptotyphosa bacterin before breeding and cows were revaccinated the next year. Leptospires were demonstrated in urine, kidney and spinal fluid of vaccinated and treated cattle. New infections occurred on range in vaccinates. Eighteen months after the last vaccination, hardjo and pomona antibody prevalences in cows were 3.6 and 3.2% respectively. A group of 250 seronegative cows on the same ranch were not vaccinated. They remained seronegative throughout the 2.5 years of the study. These cows, in contrast to infected groups, were excluded from pastures adjacent to perimeter herds and grazing leases, and they were bred by artificial inseminstion. Rotation through pastures in common with infected groups, and exposure to seropositive heat detector bulls, did not result in seroconversion in these cows. The study showed the potential of range bulls to amplify and transmit hardjo infection, limitations to the value of treatment and vaccination with available agents, and the potential of management practices to maintain an uninfected herd in close proximity to cattle carrying hardjo infection. PMID:17422729

Lipids of parasitic and saprophytic leptospires.

PubMed

Johnson, R C; Livermore, B P; Walby, J K; Jenkin, H M

1970-09-01

The lipid composition of five parasitic and six saprophytic leptospires was compared. Lipids comprise 18 to 26% of the dry weight of the cells after chloroform-methanol extraction. No residual (bound) lipid was found after acid or alkaline hydrolysis of the extracted residue. The total lipid was composed of 60 to 70% phospholipid, and the remaining lipid was free fatty acids. The phospholipid fraction contained phosphatidylethanolamine as the major component, and phosphatidylglycerol and diphosphatidylglycerol were minor components with traces of lysophatidylethanolamine sometimes found. The major fatty acids of leptospires were hexadecanoic, hexadecenoic, and octadecenoic acids. Both the unusual cis-11-hexadecenoic acid and the more common cis-9-hexadecenoic acid were synthesized by the leptospires. Neither the parasitic nor the saprophytic leptospires can chain elongate fatty acids. However, they were capable of beta-oxidation of fatty acids. Both groups of leptospires desaturate fatty acids by an aerobic pathway. When the parasite canicola was cultivated on octadecanoic acid, 87% of the hexadecenoic acid was the 11 isomer, whereas the saprophyte semeranga consisted of 10% of this isomer. In addition, the saprophytic leptospires contained more tetradecanoic acid than the parasites. No differences were observed in the lipid composition of virulent and avirulent strains of canicola.

Lipids of Parasitic and Saprophytic Leptospires

PubMed Central

Johnson, R. C.; Livermore, B. P.; Walby, Judith K.; Jenkin, H. M.

1970-01-01

The lipid composition of five parasitic and six saprophytic leptospires was compared. Lipids comprise 18 to 26% of the dry weight of the cells after chloroform-methanol extraction. No residual (bound) lipid was found after acid or alkaline hydrolysis of the extracted residue. The total lipid was composed of 60 to 70% phospholipid, and the remaining lipid was free fatty acids. The phospholipid fraction contained phosphatidylethanolamine as the major component, and phosphatidylglycerol and diphosphatidylglycerol were minor components with traces of lysophatidylethanolamine sometimes found. The major fatty acids of leptospires were hexadecanoic, hexadecenoic, and octadecenoic acids. Both the unusual cis-11-hexadecenoic acid and the more common cis-9-hexadecenoic acid were synthesized by the leptospires. Neither the parasitic nor the saprophytic leptospires can chain elongate fatty acids. However, they were capable of β-oxidation of fatty acids. Both groups of leptospires desaturate fatty acids by an aerobic pathway. When the parasite canicola was cultivated on octadecanoic acid, 87% of the hexadecenoic acid was the 11 isomer, whereas the saprophyte semeranga consisted of 10% of this isomer. In addition, the saprophytic leptospires contained more tetradecanoic acid than the parasites. No differences were observed in the lipid composition of virulent and avirulent strains of canicola. PMID:16557833

University Library Online Reference Service Program Plan, 1986/87.

ERIC Educational Resources Information Center

Koga, James S.

This program plan for online reference service--the individualized assistance provided to a library patron using an online system--at California State Polytechnic University, Pomona, covers the areas of funding, eligibility for online services, search request eligibility, database eligibility, management of online services, reference faculty…

A Portable Bioinformatics Course for Upper-Division Undergraduate Curriculum in Sciences

ERIC Educational Resources Information Center

Floraino, Wely B.

2008-01-01

This article discusses the challenges that bioinformatics education is facing and describes a bioinformatics course that is successfully taught at the California State Polytechnic University, Pomona, to the fourth year undergraduate students in biological sciences, chemistry, and computer science. Information on lecture and computer practice…

Water Quantity and Quality Processes in Urban Wetlands and Green Stormwater Management Practices

EPA Science Inventory

I have been invited to give a presentation as part of the Environmental Studies Program’s weekly seminar series at the Richard Stockton College in Pomona, NJ. I will present my dissertation research on urban wetlands and the green infrastructure research here, including the park...

RoboSimian Cuts Hole in Wall

NASA Image and Video Library

2015-06-09

Using a cordless power drill, RoboSimian cuts a hole into a panel of drywall to complete one of the tasks in the DARPA Robotics Challenge Finals in Pomona, California. This image was taken on June 6, 2015. http://photojournal.jpl.nasa.gov/catalog/PIA19326

Molecular Phylogeny of Hantaviruses Harbored by Insectivorous Bats in Côte d’Ivoire and Vietnam

PubMed Central

Gu, Se Hun; Lim, Burton K.; Kadjo, Blaise; Arai, Satoru; Kim, Jeong-Ah; Nicolas, Violaine; Lalis, Aude; Denys, Christiane; Cook, Joseph A.; Dominguez, Samuel R.; Holmes, Kathryn V.; Urushadze, Lela; Sidamonidze, Ketevan; Putkaradze, Davit; Kuzmin, Ivan V.; Kosoy, Michael Y.; Song, Jin-Won; Yanagihara, Richard

2014-01-01

The recent discovery of genetically distinct hantaviruses in multiple species of shrews and moles prompted a further exploration of their host diversification by analyzing frozen, ethanol-fixed and RNAlater®-preserved archival tissues and fecal samples from 533 bats (representing seven families, 28 genera and 53 species in the order Chiroptera), captured in Asia, Africa and the Americas in 1981–2012, using RT-PCR. Hantavirus RNA was detected in Pomona roundleaf bats (Hipposideros pomona) (family Hipposideridae), captured in Vietnam in 1997 and 1999, and in banana pipistrelles (Neoromicia nanus) (family Vespertilionidae), captured in Côte d’Ivoire in 2011. Phylogenetic analysis, based on the full-length S- and partial M- and L-segment sequences using maximum likelihood and Bayesian methods, demonstrated that the newfound hantaviruses formed highly divergent lineages, comprising other recently recognized bat-borne hantaviruses in Sierra Leone and China. The detection of bat-associated hantaviruses opens a new era in hantavirology and provides insights into their evolutionary origins. PMID:24784569

High Accuracy Ground-based near-Earth-asteroid Astrometry using Synthetic Tracking

NASA Astrophysics Data System (ADS)

Zhai, C.; Shao, M.; Saini, N. S.; Sandhu, J. S.; Werne, T. A.; Choi, P.; Ely, T. A.; Jacobs, C.; Lazio, J.; Martin-Mur, T. J.; Owen, W. K.; Preston, R. A.; Turyshev, S. G.

2017-12-01

Accurate astrometry is crucial for determining the orbits of near-Earth-asteroids (NEAs). Further, the future of deep space high data rate communications is likely to be optical communications, such as the Deep Space Optical Communications package to be carried on the Psyche Discovery mission to the Psyche asteroid. We have recently upgraded our instrument on the Pomona College 1 m telescope, at JPL's Table Mountain Facility, for conducting synthetic tracking by taking many short exposure images. These images can be then combined in post-processing to track both asteroid and reference stars to yield accurate astrometry. Utilizing the precision of the current and future Gaia data releases, the JPL-Pomona College effort is now demonstrating precision astrometry on NEAs, which is likely to be of considerable value for cataloging NEAs. Further, treating NEAs as proxies of future spacecraft that carry optical communication lasers, our results serve as a measure of the astrometric accuracy that could be achieved for future plane-of-sky optical navigation.

Creating Schools and Strengthening Communities through Adaptive Reuse.

ERIC Educational Resources Information Center

Spector, Stephen

This publication focuses on four school adaptive reuse projects--in Phoenix, Arizona; Wake County, North Carolina; Pomona, California; and Trenton, New Jersey. Together, the projects illustrate the many benefits of adaptive reuse and show that mainstream school districts can meet the regulatory and political challenges necessary to make such…

Designing Networked Improvement in a Small-College Context

ERIC Educational Resources Information Center

Rachford, Jennifer L.; Brown, Travis M.; Sambolin, Hector L., Jr.; Seligman, Lenny

2017-01-01

This chapter demonstrates the complexity of pedagogical and curricular change as it unfolds through several overlapping phases of increasingly coordinated reflection and action around STEM initiatives at Pomona College. It argues for a networked model of research and practice, drawing on theory and lessons from improvement science and highlighting…

Integration, Reflection, Interpretation: Realizing the Goals of a General Education Capstone Course

ERIC Educational Resources Information Center

Fernandez, Nancy Page

2006-01-01

For the past 23 years, students at California State Polytechnic University at Pomona have benefited from its Interdisciplinary General Education Program (IGE)--a sequential, interdisciplinary general education program that culminates in a capstone course. IGE's history and structure support a strong culture of assessment. The program, founded in…

The Dynamics of Organizational Culture and Academic Planning

ERIC Educational Resources Information Center

Willson, Richard

2006-01-01

Planning approaches are in a dynamic relationship with organizational culture. This article uses a case study of academic planning at California State Polytechnic University, Pomona to draw a correspondence between types of organizational culture and planning approaches. The case study shows the differing conceptions of organizational culture held…

Beyond the Accusation of Plagiarism

ERIC Educational Resources Information Center

Gu, Qing; Brooks, Jane

2008-01-01

The paper explores the complexity of the notion of plagiarism from sociocultural and psychological perspectives. Plagiarism is a dynamic and multi-layered phenomenon [Russikoff, K., Fucaloro, L., Salkauskiene, D., 2003. "Plagiarism as a cross-cultural phenomenon." "The CAL Poly Pomona Journal of Interdisciplinary Studies" 16, 109-120.…

Interlaboratory and between-specimen comparisons of diagnostic tests for leptospirosis in sheep and cattle.

PubMed

Fang, Fang; Collins-Emerson, Julie M; Heuer, Cord; Hill, Fraser I; Tisdall, David J; Wilson, Peter R; Benschop, Jackie

2014-11-01

A study was performed to investigate interlaboratory test agreement between a research and a commercial veterinary diagnostic laboratory on blood and urine samples, and to investigate test agreement between blood, urine, and kidney samples (research laboratory) for leptospirosis diagnosis. Samples were sourced from 399 sheep and 146 beef cattle from a local abattoir. Interlaboratory agreement for real-time quantitative polymerase chain reaction (qPCR) results on urine samples was almost perfect (kappa = 0.90), despite the use of different amplification targets (DNA gyrase subunit B gene vs. 16s ribosomal RNA gene), chemistries (SYTO9 vs. TaqMan probe), and pre-PCR processing. Interlaboratory agreement for microscopic agglutination test (MAT) positivity was almost perfect (kappa = 0.93) for Leptospira borgpetersenii serovar Hardjo subtype Hardjobovis (Hardjobovis) but moderate (kappa = 0.53) for Leptospira interrogans serovar Pomona (Pomona). Among animals that had different titers recorded, higher Hardjobovis and lower Pomona titers were reported by the commercial laboratory than by the research laboratory (P < 0.005). These interlaboratory comparisons can assist researchers and diagnosticians in interpreting the sometimes discrepant test results. Within the research laboratory, the comparison of qPCR results on urine and kidney showed almost perfect agreement (kappa = 0.84), suggesting that the qPCR on these 2 specimens can be used interchangeably. The agreement between MAT positivity and urine and kidney qPCR results was fair (kappa = 0.32 and kappa = 0.33, respectively). However, the prevalence ratio of urine and kidney qPCR positivity in Hardjobovis-seropositive versus Hardjobovis-seronegative sheep indicated that Hardjobovis seropositivity found in sheep may be able to predict shedding or renal carriage. © 2014 The Author(s).

Healthy Persons with Intellectual Disabilities in an Inclusive Society

ERIC Educational Resources Information Center

van Schrojenstein Lantman-de Valk, H. M. J.

2009-01-01

Persons with intellectual disabilities (ID) have twice as many health problems than the general public. The author discusses evidence-based research on prevalence and best treatment of primary and secondary health problems in persons with ID. Citing the pan-European Pomona project, European data were collected on training for health professionals…

High School Students Compete in Solar-Powered Bike Race

Science.gov Websites

bicycle race designed to give students hands-on learning experiences with renewable energy technologies . What: The Colorado Solar BikeRayce is a competition for high school students to race solar-powered ) sponsors the competition. Who: High school students from Bollman Occupational Center (Thornton) and Pomona

75 FR 45114 - Determination of Insufficient Assets To Satisfy Claims Against Financial Institution in Receivership

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-02

...: Notice. SUMMARY: The FDIC has determined that insufficient assets exist in the receivership of PFF Bank... Claims Agent at (972) 761-8677. Written correspondence may also be mailed to FDIC as Receiver of PFF Bank... November 21, 2008, PFF Bank & Trust, Pomona, California, (FIN 10024) was closed by the Office of Thrift...

The prevalence of leptospirosis and its association with multifocal interstitial nephritis in swine at slaughter.

PubMed Central

Baker, T F; McEwen, S A; Prescott, J F; Meek, A H

1989-01-01

An abattoir survey was undertaken to determine the prevalence of leptospirosis and its association with lesions of multifocal interstitial nephritis (so-called "white spotted kidneys") in swine at slaughter. Both cross-sectional and case-control study designs were used. Of 197 kidneys from hogs randomly selected at slaughter, 11 (5.6%) had generalized grey-white foci typical of multifocal interstitial nephritis (MFIN). Antibody titers greater than or equal to 1:80 against Leptospira pomona were detected in nine (4.6%) hogs and against L. bratislava in 63 (32%) of these hogs. Leptospira pomona (kennewicki) was detected by immunofluorescence in 5/197 (2.5%) of randomly selected hogs. Leptospires identified as genotype kennewicki were isolated from six (9.8%) of 61 kidneys cultured. Leptospira bratislava was not detected by immunofluorescence or culture. There was a highly significant (p = 0.00) and strong association (odds ratio (OR) = 195) between high L. pomona titer (greater than or equal to 1:80) and the presence of leptospires in the kidneys, as detected by culture. There was also a significant (p = 0.046) and strong (OR = 8.10) association between multifocal interstitial nephritis and the presence of renal leptospires as detected by culture. The association between leptospiral titer and MFIN lesions in the prevalence survey group of animals was statistically significant (p = 0.031), but this association was not significant in the case-control study group (p = 0.071) The failure to identify L. bratislava despite serological evidence of infection suggests that some of these seropositive animals may have been transiently infected at an early age, that serological findings were falsely positive, or that immunofluorescence and isolation attempts failed to detect L. bratislava if they were indeed present in the kidneys.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:2766150

Undocumented in Honors

ERIC Educational Resources Information Center

Aramburo, Kimberly; Bhavsar, Suketu

2013-01-01

During her time at the Kellogg Honors College at Cal Poly Pomona, Suketu Bhavsar has encountered several high-achieving students who, after coming to trust her, have revealed themselves to her as undocumented. These students came to the United States as children through non-legal channels, generally brought by their families, who were searching…

The Egalitarianism of Honors at a Polytechnic University

ERIC Educational Resources Information Center

Coley, Soraya M.

2015-01-01

The Kellogg Honors College (KHC) is a distinctive community within Cal Poly Pomona (CPP), a public university in Southern California and one of twenty-three universities in the California State University (CSU) system. With over 22,000 students, CPP is the second-largest polytechnic university in the United States. The university's goal is to…

Strengthening At-Risk and Homeless Young Mothers and Children

ERIC Educational Resources Information Center

Medeiros, Debra; Vaulton, Wendy

2010-01-01

The Strengthening At Risk and Homeless Young Mothers and Children Initiative, funded by the Conrad N. Hilton Foundation, aims to improve the housing, health, and development of homeless and at-risk young families. This article describes the services provided in four program sites (Pomona, CA; Antelope Valley, CA; Minneapolis, MN; and Chicago, IL)…

The Costs and Benefits of Deferred Giving.

ERIC Educational Resources Information Center

Fink, Norman S.; Metzler, Howard C.

It is argued in this book that while there can be a significant payoff for deferred giving programs, it is important to determine their cost effectiveness. Modern business methods of cost accounting, benefits analysis, and actuarial and econometric forecasting are applied to the Pomona College plan, whose study was supported by Lilly Endowment,…

Designing an Introductory CIS Course to Attract and Retain Female (and Male) Students

ERIC Educational Resources Information Center

Soe, Louise; Guthrie, Ruth; Yakura, Elaine; Hwang, Drew

2012-01-01

In order to stem the decline of female majors and encourage the persistence of all students in the Computer Information Systems (CIS) Department at Cal Poly Pomona (CPP), the department faculty is instituting a new course to introduce incoming freshmen and transfer students into the major. This course will incorporate the career exploration that…

High Accuracy Ground-based near-Earth-asteroid Astrometry using Synthetic Tracking

NASA Astrophysics Data System (ADS)

Zhai, Chengxing; Shao, Michael; Saini, Navtej; Sandhu, Jagmit; Werne, Thomas; Choi, Philip; Ely, Todd A.; Jacobs, Chirstopher S.; Lazio, Joseph; Martin-Mur, Tomas J.; Owen, William M.; Preston, Robert; Turyshev, Slava; Michell, Adam; Nazli, Kutay; Cui, Isaac; Monchama, Rachel

2018-01-01

Accurate astrometry is crucial for determining the orbits of near-Earth-asteroids (NEAs). Further, the future of deep space high data rate communications is likely to be optical communications, such as the Deep Space Optical Communications package that is part of the baseline payload for the planned Psyche Discovery mission to the Psyche asteroid. We have recently upgraded our instrument on the Pomona College 1 m telescope, at JPL's Table Mountain Facility, for conducting synthetic tracking by taking many short exposure images. These images can be then combined in post-processing to track both asteroid and reference stars to yield accurate astrometry. Utilizing the precision of the current and future Gaia data releases, the JPL-Pomona College effort is now demonstrating precision astrometry on NEAs, which is likely to be of considerable value for cataloging NEAs. Further, treating NEAs as proxies of future spacecraft that carry optical communication lasers, our results serve as a measure of the astrometric accuracy that could be achieved for future plane-of-sky optical navigation.

KAPAO-Alpha: An On-The-Sky Testbed for Adaptive Optics on Small Aperture Telescopes

NASA Astrophysics Data System (ADS)

Morrison, Will; Choi, P. I.; Severson, S. A.; Spjut, E.; Contreras, D. S.; Gilbreth, B. N.; McGonigle, L. P.; Rudy, A. R.; Xue, A.; Baranec, C.; Riddle, R.

2012-05-01

We present initial in-lab and on-sky results of a natural guide star adaptive optics instrument, KAPAO-Alpha, being deployed on Pomona College’s 1-meter telescope at Table Mountain Observatory. The instrument is an engineering prototype designed to help us identify and solve design and integration issues before building KAPAO, a low-cost, dual-band, natural guide star AO system currently in active development and scheduled for first light in 2013. The Alpha system operates at visible wavelengths, employs Shack-Hartmann wavefront sensing, and is assembled entirely from commercially available components that include: off-the-shelf optics, a 140-actuator BMC deformable mirror, a high speed SciMeasure Lil’ Joe camera, and an EMCCD for science image acquisition. Wavefront reconstruction operating at 1-kHz speeds is handled with a consumer-grade computer running custom software adopted from the Robo-AO project. The assembly and integration of the Alpha instrument has been undertaken as a Pomona College undergraduate thesis. As part of the larger KAPAO project, it is supported by the National Science Foundation under Grant No. 0960343.

Immunization Schedules for Adults

MedlinePlus

... color [2 pages] PDF black & white [2 pages] Spanish Version (en español) Vacunas recomendadas para adultos según ... easy-to-read format in English and/or Spanish on your website. See examples of how the ...

Hepatitis B Vaccine: What You Need to Know

MedlinePlus

... información sobre vacunas están disponibles en español y en muchos otros idiomas. Visite www. immunize. org/ vis 1 Why get vaccinated? Hepatitis B is a serious disease that affects the liver. ...

Points of View: A Survey of Survey Courses--Are They Effective? A Case for Nonsurvey Introductory Biology Courses

ERIC Educational Resources Information Center

Becker, David

2005-01-01

In spring of 1998, the Biology Department at Pomona College changed from a two-semester survey introductory biology sequence to a core set of three courses, none of which is a traditional survey course. They had been wrestling for several years with a number of issues regarding the survey courses, including (1) what topics to include and exclude;…

Current Issues in Mental Retardation and Human Development: Selected Papers from the 1970 Staff Development Conferences of the President's Committee on Mental Retardation (Washington, D.C., 1971).

ERIC Educational Resources Information Center

Stedman, Donald J., Ed.

Six papers discuss some of the current issues in the field of mental retardation and human development. Epidemiology of mental retardation from a sociological and clinical point of view is analyzed by Jane R. Mercer, based on studies of mental retardation in the community in Pomona, California. The role of genetics and intra-uterine diagnosis of…

In California, 2 Small Colleges Abound in Fulbright Scholars

ERIC Educational Resources Information Center

Hermes, J. J.

2007-01-01

Nestled at the base of the San Gabriel Mountains about 35 miles east of Los Angeles, a small liberal-arts college is establishing itself as a factory for a coveted international export: students who land Fulbright scholarships to teach or do research abroad. In the last four years, 48 students from Pomona College, which enrolls about 1,500…

A "Do It Yourself Kit" for Implementing a High School Peer Counseling Program in Three Easy Steps!

ERIC Educational Resources Information Center

Marsico, John; Nelson, Judith

Selected high school sophomores and juniors at Pomona Senior High School in Arvada, Colorado, are trained in counseling and group communication skills in order to be able to facilitate small peer groups in elective classes. The purpose is to help students find personal meaning in their lives and be more successful in school. An attempt is made to…

Canine leptospirosis in Switzerland-A prospective cross-sectional study examining seroprevalence, risk factors and urinary shedding of pathogenic leptospires.

PubMed

Delaude, Alessandro; Rodriguez-Campos, Sabrina; Dreyfus, Anou; Counotte, Michel Jacques; Francey, Thierry; Schweighauser, Ariane; Lettry, Sophie; Schuller, Simone

2017-06-01

Leptospirosis is an important worldwide zoonosis. While human leptospirosis remains rare in Switzerland, the incidence of canine leptospirosis is unusually high compared to other European countries. The aims of this cross-sectional study were to determine the exposure of asymtomatic dogs to pathogenic Leptospira in Switzerland, to characterise risk factors associated with seropositivity and to determine the prevalence of urinary shedding. Sampling was stratified to cover the whole of Switzerland. Sera were tested by microscopic agglutination test for antibodies against a panel of 12 serovars. Urine was tested for pathogenic Leptospira using a LipL32 real-time PCR. Of 377 sera, 55.7% (95%CI 51.2-60.7) showed a reciprocal MAT titre of ≥1:40 and 24.9% (95%CI 20.7-29.4) of ≥1:100 to at least one serovar. Seropositivity (MAT ≥1:100) was most common to serovars Australis (14.9%; 95% CI 11.4-18.6) and Bratislava (8.8%; 95%CI 6.1-11.7), followed by Copenhageni (6.1%; 95%CI 3.7-8.5), Canicola (5%; 95%CI 2.9-7.4), Grippotyphosa (4.5%; 95%CI 2.7-6.9), Pomona (4%; 95%CI 2.1-6.1), Autumnalis (2.7%; 95%CI 1.3-4.2) and Icterohaemorrhagiae (1.6%; 95%CI 0.5-2.9). In unvaccinated dogs (n=84) the prevalence of a MAT titre ≥100 was 17.9% (95%CI 10.7-26.2), with a similar distribution of reactive serovars. Variables associated with seropositivity (≥1:40) to any serovar included age (OR 1.29/year; 95%CI: 1.1-1.5) and bioregion with higher risks in the regions Northern Alps (OR 14.5; 95%CI 2.2-292.7), Central Plateau (OR 12.3; 95%CI 2.0-244.1) and Jura (OR 11.2; 95%CI 1.7-226.7) compared to Southern Central Alps. Dogs living with horses were significantly more likely to have antibodies to serovar Bratislava (OR 4.68;95%CI 1.2-17.2). Hunting was a significant risk factor for seropositivtiy to serovar Grippotyphosa (OR 8.03; 95%CI 1.6-30.8). Urine qPCR positivity was uncommon (1/408 dogs; 0.2%; 95% CI0-0.7). These results demonstrate that dogs in Switzerland are commonly exposed

Androgen Deprivation Therapy and Cognitive Impairment

DTIC Science & Technology

2017-08-01

development of new strategies to optimize the physical and mental health of men with prostate cancer and improve the quality of life and well-being...CONTRACTING ORGANIZATION: Western University of Health Sciences Pomona, CA 91766 REPORT DATE: August 2017 TYPE OF REPORT: Annual PREPARED FOR...NAME(S) AND ADDRESS(ES) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION REPORT NUMBER Western University of Health Sciences 309 East Second Street

[Leptospirosis outbreak in calves from Corrientes Province, Argentina].

PubMed

Draghi, María G; Brihuega, Bibiana; Benítez, Daniel; Sala, Juan M; Biotti, Graciela M; Pereyra, Matilde; Homse, Alberto; Guariniello, Luciano

2011-01-01

Leptospirosis is an infectious disease resulting in significant economic losses in livestock production. This disease causes abortion, embryo death, death of calves within the first few days of life and mastitis. We report a leptospirosis outbreak in calf growing and fattening. Histopathological and hemoparasite studies, immunofluorescence, and bacterial cultures were performed. A strain of Leptospira interrogans serovar Pomona was isolated from samples collected from dead calves.

A novel tetravalent Leptospira bacterin protects against infection and shedding following challenge in dogs

PubMed Central

Klaasen, H. L. B. M.; van der Veen, M.; Molkenboer, M. J. C. H.; Sutton, D.

2013-01-01

Recent evidence based on the current epidemiological situation suggests that vaccines against canine leptospirosis in Europe should be directed against infection with Leptospira interrogans (sensu lato) serogroups Canicola, Icterohaemorrhagiae, Grippotyphosa and Australis. In the eight studies presented here, dogs were vaccinated with Nobivac L4 (MSD Animal Health), a new tetravalent inactivated vaccine containing antigen from four strains representing these four serogroups. The dogs were then challenged, together with unvaccinated control dogs, using heterologous strains from the same four serogroups. In four of the studies, pups without agglutinating antibodies against the four serogroups were vaccinated with Nobivac L4 vaccine. In a further four studies, Nobivac L4 vaccine was given 48 hours after administration of antiserum from vaccinated dogs designed to mimic the serological status of pups with maternally derived antibodies against these serogroups. In all eight studies, vaccine efficacy was assessed in terms of antibody response, clinical signs, fever, thrombocyte count, frequency of positive isolation of challenge organisms from blood, urine and kidney and frequency of interstitial nephritis. The results demonstrate that Nobivac L4 vaccine induces sterile immunity against leptospiraemia and renal infection with strains of serogroups Canicola, Icterohaemorrhagiae and Grippotyphosa, and induces sterile immunity against leptospiraemia with a strain of serogroup Australis. Since sterile immunity was achieved in pups pretreated with antiserum as well, it can be concluded that this vaccine is also likely to be efficacious in the face of maternally derived antibodies in pups from the age of six weeks. PMID:23180149

Isolation and clinical sample typing of human leptospirosis cases in Argentina.

PubMed

Chiani, Yosena; Jacob, Paulina; Varni, Vanina; Landolt, Noelia; Schmeling, María Fernanda; Pujato, Nazarena; Caimi, Karina; Vanasco, Bibiana

2016-01-01

Leptospira typing is carried out using isolated strains. Because of difficulties in obtaining them, direct identification of infective Leptospira in clinical samples is a high priority. Multilocus sequence typing (MLST) proved highly discriminatory for seven pathogenic species of Leptospira, allowing isolate characterization and robust assignment to species, in addition to phylogenetic evidence for the relatedness between species. In this study we characterized Leptospira strains circulating in Argentina, using typing methods applied to human clinical samples and isolates. Phylogenetic studies based on 16S ribosomal RNA gene sequences enabled typing of 8 isolates (6 Leptospira interrogans, one Leptospira wolffii and one Leptospira broomii) and 58 out of 85 (68.2%) clinical samples (55 L. interrogans, 2 Leptospira meyeri, and one Leptospira kirschneri). MLST results for the L. interrogans isolates indicated that five were probably Canicola serogroup (ST37) and one was probably Icterohaemorrhagiae serogroup (ST17). Eleven clinical samples (21.6%), provided MLST interpretable data: five were probably Pyrogenes serogroup (ST13), four Sejroe (ST20), one Autumnalis (ST22) and one Canicola (ST37). To the best of our knowledge this study is the first report of the use of an MLST typing scheme with seven loci to identify Leptospira directly from clinical samples in Argentina. The use of clinical samples presents the advantage of the possibility of knowing the infecting strain without resorting to isolates. This study also allowed, for the first time, the characterization of isolates of intermediate pathogenicity species (L. wolffii and L. broomii) from symptomatic patients. Copyright © 2015 Elsevier B.V. All rights reserved.

The Evaluation of Jet Injection for use in Veterinary Medicine,

DTIC Science & Technology

1976-05-01

therapeutic drugs. As the demand for food animal protein increases and as pet aninal populations increase, technical advances into more advantageous methods of...general areas at the present time. 1) As society’s demand for meat protein increases, mass production methods will become increasingly necessary in...vaccine, modified live virus, bovine t.nsue culture ’rigin, Leptospira pomona bacterin, Anciv- :;.erum Co., Division of Phillips Roxane, St. Toseph, Mo

Team RoboSimian

NASA Image and Video Library

2015-06-09

Many members of Team RoboSimian and a few guests gather with competition hardware at a "Meet the Robots" event during the DARPA Robotics Challenge Finals in Pomona, California, on June 6, 2015. The RoboSimian team at JPL is collaborating with partners at the University of California, Santa Barbara, and the California Institute of Technology in Pasadena. Caltech manages JPL for NASA. http://photojournal.jpl.nasa.gov/catalog/PIA19329

Description of a new tick species, Ixodes collaris n. sp. (Acari: Ixodidae), from bats (Chiroptera: Hipposideridae, Rhinolophidae) in Vietnam.

PubMed

Hornok, Sándor; Görföl, Tamás; Estók, Péter; Tu, Vuong Tan; Kontschán, Jenő

2016-06-10

In a recent study on ixodid bat ticks from Eurasia, a high genetic difference was found between Ixodes vespertilionis from Europe and Vietnam. Accordingly, it was proposed that I. vespertilionis is a species complex, with at least one additional, hitherto undescribed species. The aim of the present study was to investigate the morphology of bat ticks from Vietnam and to assess their taxonomic status in comparison with those collected in Europe. Ixodid bat ticks (two females and two nymphs) collected from the pomona leaf-nosed bat (Hipposideros pomona) (Hipposideridae) and intermediate horseshoe bat (Rhinolophus affinis) (Rhinolophidae) in Vietnam showed major morphological differences from European isolates of I. vespertilionis, including the shape of the scutum, the enclosure and shape of porose areas, the presence of a caudo-lateral collar-like ridge ventrally on the basis capituli, polytrich coxae with short setae, and grouped (non-linear) arrangement of anterior pit sensillae in Haller's organ. In this study the female and the nymph of an ixodid bat tick species from Vietnam are described for the first time. The genetic and morphological differences between I. vespertilionis Koch, 1844 and these bat ticks from Vietnam justify the status of the latter as a distinct species, Ixodes collaris Hornok n. sp.

KAPAO: a MEMS-based natural guide star adaptive optics system

NASA Astrophysics Data System (ADS)

Severson, Scott A.; Choi, Philip I.; Contreras, Daniel S.; Gilbreth, Blaine N.; Littleton, Erik; McGonigle, Lorcan P.; Morrison, William A.; Rudy, Alex R.; Wong, Jonathan R.; Xue, Andrew; Spjut, Erik; Baranec, Christoph; Riddle, Reed

2013-03-01

We describe KAPAO, our project to develop and deploy a low-cost, remote-access, natural guide star adaptive optics (AO) system for the Pomona College Table Mountain Observatory (TMO) 1-meter telescope. We use a commercially available 140-actuator BMC MEMS deformable mirror and a version of the Robo-AO control software developed by Caltech and IUCAA. We have structured our development around the rapid building and testing of a prototype system, KAPAO-Alpha, while simultaneously designing our more capable final system, KAPAO-Prime. The main differences between these systems are the prototype's reliance on off-the-shelf optics and a single visible-light science camera versus the final design's improved throughput and capabilities due to the use of custom optics and dual-band, visible and near-infrared imaging. In this paper, we present the instrument design and on-sky closed-loop testing of KAPAO-Alpha as well as our plans for KAPAO-Prime. The primarily undergraduate-education nature of our partner institutions, both public (Sonoma State University) and private (Pomona and Harvey Mudd Colleges), has enabled us to engage physics, astronomy, and engineering undergraduates in all phases of this project. This material is based upon work supported by the National Science Foundation under Grant No. 0960343.

KAPAO Prime: Design and Simulation

NASA Astrophysics Data System (ADS)

McGonigle, Lorcan

2012-11-01

KAPAO (KAPAO A Pomona Adaptive Optics instrument) is a dual-band natural guide star adaptive optics system designed to measure and remove atmospheric aberration from Pomona College's telescope atop Table Mountain. We present here, the final optical system, referred to as Prime, designed in Zemax Optical Design Software. Prime is characterized by diffraction limited imaging over the full 73'' field of view of our Andor Camera at f/33 as well as for our NIR Xenics camera at f/50. In Zemax, tolerances of 1% on OAP focal length and off-axis distance were shown to contribute an additional 4 nm of wavefront error (98% confidence) over the field of view of the Andor camera; the contribution from surface irregularity was determined analytically to be 40nm for OAPs specified to l/10 surface irregularity. Modeling of the temperature deformation of the breadboard in SolidWorks revealed 70 micron contractions along the edges of the board for a decrease of 75 F; when applied to OAP positions such displacements from the optimal layout are predicted to contribute an additional 20 nanometers of wavefront error. Flexure modeling of the breadboard due to gravity is on-going. We hope to begin alignment and testing of ``Prime'' in Q1 2013.

Leptospirosis in Sheep in Western Canada

PubMed Central

Kingscote, B.

1985-01-01

A survey of 930 ovine sera and kidneys from 33 sheep was conducted to assess the rate of leptospiral infection in sheep slaughtered in Alberta. Sera were tested for the presence of agglutinins to indigenous serovars of Leptospira interrogans. Kidneys with gross lesions were examined for the presence of leptospires by means of an indirect fluorescent antibody test (FAT) and by culture. Antibodies to serovars pomona and hardjo were present at rates of 1.0% and 0.4%, respectively, in sheep from Saskatchewan, Alberta and British Columbia. Sera from 120 feedlot lambs shipped from Oregon reacted to serovars pomona, hardjo and grippotyphosa at rates of 1.7%, 61.7% and 59.1%, respectively. Fluorescent antibody test detected serovars (presumptively) hardjo in 52% of Oregon feedlot lambs and grippotyphosa in 32% of the same group, a finding supported by the isolation of both these serovars from a pool of two fluorescent antibody test-positive kidneys. The grippotyphosa strain was highly virulent for hamsters, producing intense icterus and death. Leptospires, presumptively serovar grippotyphosa were demonstrated by fluorescent antibody test in one Alberta lamb kidney. The possibility of spreading leptospirosis by movement of breeding stock through public facilities and by assembling lambs in feedlots is discussed. PMID:17422531

Authorized and Operating Purposes of Corps of Engineers Reservoirs

DTIC Science & Technology

1992-07-01

Puerto Rico CERRILLOS DAM AND RESERVOIR Jacksonville E-9O PORTUGUES DAM AND RESERVOIR Jacksonville E-92 South Carolina HARTWELL DAM AND LAKE Savannah E...LAKE Missouri Kansas City E-12 POMONA LAKE Kansas Kansas City E-12 PORTUGUES DAM AND RESERVOIR Puerto Rico Jacksonville E-92 PRADO DAM (SANTA ANA...PROJECT Florida Jacksonville E-92 PORTUGUES DAM AND RESERVOIR Puerto Rico Jacksonville E-92 RODMAN LOCK AND DAM (CROSS FLORIDA BARGE CANAL Florida

NBodyLab: A Testbed for Undergraduates Utilizing a Web Interface to NEMO and MD-GRAPE2 Hardware

NASA Astrophysics Data System (ADS)

Johnson, V. L.; Teuben, P. J.; Penprase, B. E.

An N-body simulation testbed called NBodyLab was developed at Pomona College as a teaching tool for undergraduates. The testbed runs under Linux and provides a web interface to selected back-end NEMO modeling and analysis tools, and several integration methods which can optionally use an MD-GRAPE2 supercomputer card in the server to accelerate calculation of particle-particle forces. The testbed provides a framework for using and experimenting with the main components of N-body simulations: data models and transformations, numerical integration of the equations of motion, analysis and visualization products, and acceleration techniques (in this case, special purpose hardware). The testbed can be used by students with no knowledge of programming or Unix, freeing such students and their instructor to spend more time on scientific experimentation. The advanced student can extend the testbed software and/or more quickly transition to the use of more advanced Unix-based toolsets such as NEMO, Starlab and model builders such as GalactICS. Cosmology students at Pomona College used the testbed to study collisions of galaxies with different speeds, masses, densities, collision angles, angular momentum, etc., attempting to simulate, for example, the Tadpole Galaxy and the Antenna Galaxies. The testbed framework is available as open-source to assist other researchers and educators. Recommendations are made for testbed enhancements.

Pilot Cross-Sectional Study of Three Zoonoses (Lyme Disease, Tularaemia, Leptospirosis) among Healthy Blood Donors in Eastern Slovakia.

PubMed

Zákutná, Ľubica; Dorko, Erik; Rimárová, Kvetoslava; Kizeková, Marianna

2015-06-01

The aim of the study was to determine the seroprevalence of three zoonotic infections among healthy blood donors/volunteers in Eastern Slovakia. Sera from 124 blood donors were investigated for the presence of antibodies against Borrelia burgdorferi, Francisella tularensis and Leptospira pomona. The participants also completed the questionnaire about demographic, exposure and epidemiological characteristics. Two serological methods were used for the diagnosis: the enzyme linked protein A/G assay (ELPAGA) and the Western blot (WB). First, sera were screened by ELPAGA (except for leptospirosis). The observed seroprevalence was 15% for Lyme borreliosis (LB) and 4% for tularaemia (TUL). The results were confirmed by WB. Positive IgG antibodies (WB method) were detected only in 1.6% of examined for LB and 0.8% for TUL. Our results did not identify any antibodies against Leptospira pomona agent in the examined healthy blood donors group. ELPAGA seroprevalence for TUL was significantly higher in blood donors working in the agricultural area in the direct contact with hay, straw, manure, and agricultural land. Our outputs determine tick bite as a significant risk factor for LB. The study confirms the explosion of tick-borne diseases in the healthy population of people. The exposure risk for leptospirosis seems to be minimal. Copyright© by the National Institute of Public Health, Prague 2015.

Some Observations on the Diagnosis and Epidemiology of Leptospirosis in Swine

PubMed Central

Mitchell, D.; Robertson, A.; Corner, A. H.; Boulanger, P.

1966-01-01

The results of combined epidemiological, clinical, serological, bacteriological and histopathological studies following an outbreak of disease caused by L. pomona on a farm stocked with cattle, sheep, pigs, goats and horses maintained for experimental purposes, are reported. The incidence of infection was high in horses, cattle and pigs. A few low titres were seen in sheep. The goats were not infected. Apart from a single bovine abortion all the clinical symptoms observed occurred in pregnant sows. Seven of these aborted or gave birth to stillborn pigs within a six week period. Fifteen species of wildlife were trapped or shot on the farm during the year following the outbreak. L. pomona was isolated from four skunks and a porcupine. Epidemiological studies indicated that wildlife reservoir hosts were the primary source of infection for the domestic livestock. Leptospiruria and the serological response were studied in a group of eight infected sows. Microscopic agglutination titres of 102 or less could not be associated with leptospiruria and the duration of leptospiruria was found to range from a few weeks to over two years in individual sows. Direct dark-field examination of urine proved superior to guinea-pig inoculation as a method of detecting leptospiruria and it is suggested that the former technique could be adopted with advantage as a routine aid to diagnosis. PMID:4226190

Cultural Resources Survey of Fall River Lake, Kansas.

DTIC Science & Technology

1979-07-01

vessel 1s a globular jar with a constricted orifice. The pottery 1s most often undecorated but when decorated with Incision , 1t occurs mainly on the...stream and east of Fall River. Both phases are represented by pottery (1 Butler sherd and 2 Pomona Ware). Also found was a plano -convex endscraper...Additionally, a longitudinal Incised grooved boatstone was found which according to Flnnegan and Witty (1977:25) 1s "Woodland" 1n time and culture. It

The Pomona-Rincon Road and Its Place in the Regional Transportation Network

DTIC Science & Technology

1989-10-19

on American soil by diverting north of Pilot Knob (near Yuma in Imperial County) to Indian Wells, near Indio. This proved impossible, due to desert...Overland Mail, and then the Fort Yuma to Los Angeles Road. Portions of this route are still extant in the Prado Basin south of Euclid Avenue. The Serrano...Emigrant Trail. It became the route of the Butterfield Overland Mail, and then the Fort Yuma to Los Angeles Road. Portions of this route are still extant in

Characterization of Structural and Pigmentary Colors in Common Emigrant (Catopsilia Pomona) Butterfly

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ghate, Ekata; Kulkarni, G. R.; Bhoraskar, S. V.

2011-10-20

Study of structural colors in case of insects and butterflies is important for their biomimic and biophotonics applications. Structural color is the color which is produced by physical structures and their interaction with light while pigmentary color is produced by absorption of light by pigments. Common Emigrant butterfly is widely distributed in India. It is of moderate size with wing span of about 60-80 mm. The wings are broadly white with yellow or sulphur yellow coloration at places as well as few dark black patches. It belongs to family Pieridae. A study of structural color in case of Common Emigrantmore » butterfly has been carried out in the present work. The characterization of wing color was performed using absorption spectroscopy. Scanning electron microscopic study of the wings of Common Emigrant butterfly showed that three different types of scales are present on the wing surface dorsally. Diffracting structures are present in certain parts of the surfaces of the various scales. Bead like structures are embedded in the intricate structures of the scales. Absorption spectra revealed that a strong absorption peak is seen in the UV-range. Crystalline structure of beads was confirmed by the X-ray diffraction analysis.« less

Immune Responses and Protection of Aotus Monkeys Immunized with Irradiated Plasmodium vivax Sporozoites

DTIC Science & Technology

2011-01-01

The experimental protocol was approved by the Animal Ethical Committee of the Universidad del Valle (Cali). Parasite and irradiation. Plasmodium...three repeats ofpll (GDRADGPA) and (ANGAGNQPG) sequences, derived from VK210 and VK247 CS variants, respectively. A non-malaria related peptide Ptt30...Universidad del Valle and Centro Internacional de Vacunas, Cali, Colombia, E-mails: alejovi@hotmail.com, anbonelo@yahoo.com, alejcaste@yahoo.com

Leptospira seroprevalence and associations between seropositivity, clinical disease and host factors in horses

PubMed Central

Båverud, V; Gunnarsson, A; Engvall, E Olsson; Franzén, P; Egenvall, A

2009-01-01

Background A cross-sectional study was carried out to determine the seroprevalence of different serovars of Leptospira spp. and their association with clinical disease and host factors in Swedish horses. Methods Sera from 2017 horses brought to equine clinics during 1997–98 were investigated. The sera were examined by microscopic agglutination test for the presence of antibodies against the following L. interrogans serovars: Bratislava strain Jez, Icterohaemorrhagiae strain Kantorowicz and Pomona strain Pomona and also L. kirschneri sv Grippotyphosa strain Duyster and L. borgpetersenii sv Sejroe strain M 84. Host factors, disease factors, season, pasture access and outdoor confinement variables were analysed with respect to seropositivity to sv Bratislava and Icterohaemorrhagiae. Multivariable logistic regression was used to model seropositivity to sv Bratislava and Icterohaemorrhagiae (seroprevalence > 8%). Results The seroprevalence, at a cut-off 1:100, were for sv Bratislava (16.6%), Icterohaemorrhagiae (8.3%), Sejroe (1.2%), Pomona (0.5%) and Grippotyphosa (0.4%). In the multivariable analysis, it was demonstrated that seroprevalence increased with age for sv Bratislava and Icterohaemorrhagiae. For sv Bratislava the seasons April – June and October – December and for sv Icterohaemorrhagiae October – December had higher seroprevalences than other seasons. Horses not used for racing had higher levels of seropositivity to sv Bratislava. Furthermore, horses with respiratory problems as well as horses with fatigue had higher levels of seropositivity to sv Bratislava. Ponies and coldbloods, and horses with access to pasture, had lower seroprevalence for sv Icterohaemorrhagiae. Healthy horses had lower seroprevalence for sv Icterohaemorrhagiae, than non-healthy horses. Conclusion There was no significant association between clinical signs and disease and positive titres to sv Bratislava (except for the association between respiratory problems and fatigue and

The California-Arizona Minority Partnership for Astronomy Research and Education (CAMPARE): Partnering Students to Astronomy at the University of Arizona's Astronomy Camp

NASA Astrophysics Data System (ADS)

Saldivar, Hector; McCarthy, D.; Rudolph, A. L.

2012-01-01

The California-Arizona Minority Partnership for Astronomy Research and Education (CAMPARE) is an NSF-funded partnership between the Astronomy Program at Cal Poly Pomona and the University of Arizona Steward Observatory designed to promote participation of underrepresented minorities, including women, in astronomy research and education. By means of this program, Cal Poly Pomona undergraduates that are either Physics majors or minors are qualified to participate in the program alongside graduate students from the University of Arizona as a camp counselor at the University of Arizona's Astronomy Camp, one of the elite astronomy programs worldwide. Students that participate in the CAMPARE program are granted an opportunity to work in a hands-on environment by teaching astronomy to students from all over the world in a highly structured environment. The CAMPARE student selected for this program in Summer 2011 worked under the supervision of Dr. Don McCarthy, professor at the University of Arizona and Astronomy Camp director for over 20 years, learning to lead a group of students through daily activities and ensure that the students are learning to their maximum potential. Through this experience, the CAMPARE student learned to capture students’ interest in astronomy and was introduced to real life teaching, which has helped prepare him for future experiences to come. We acknowledge the NSF for funding under Award No. AST-0847170, a PAARE Grant for the Calfornia-Arizona Minority Partnership for Astronomy Research and Education (CAMPARE).

Ocular penetration of intravenously administered enrofloxacin in the horse.

PubMed

Divers, T J; Irby, N L; Mohammed, H O; Schwark, W S

2008-03-01

Information on antibiotic concentrations in the equine eye following systemic therapy is limited. Reports that Leptospira spp. are frequently present in the eyes of horses with recurrent uveitis, emphasises a need for studies on ocular concentrations of specific antibiotics. 1) Enrofloxacin, administered i.v. at 7.5 mg/kg bwt q. 24 h, results in aqueous humour concentrations greater than the reported minimum inhibitory concentration (MIC) for Leptospira pomona. 2) Aqueous humour paracentesis sufficiently disrupts the blood-aqueous humour barrier (BAB) to cause an increase in aqueous humour protein and enrofloxacin concentrations. Aqueous humour enrofloxacin and total protein concentrations were determined in 6 healthy, mature horses after i.v. administration of enrofloxacin. Paracentesis was performed on the left eye on Days 3 and 4, 1 h following enrofloxacin administration, to determine enrofloxacin concentrations in healthy eyes and in eyes with mechanical disruption of the BAB. Paracentesis was also performed on the right eye 23 h after enrofloxacin administration. Blood samples were collected from the horses at identical times to determine enrofloxacin aqueous humour:plasma ratios. Mean +/- s.d. enrofloxacin concentration in the aqueous humour 1 h post administration on Day 3 was 0.32 +/- 0.10 mg/l (range 0.18-0.47); and aqueous humour enrofloxacin, total protein and aqueous humour:plasma enrofloxacin ratios were higher on Day 4 than Day 3. Following disruption of the BAB, enrofloxacin concentrations were above the reported MIC for Leptospira pomona.

KAPAO Prime: Design and Simulation

NASA Astrophysics Data System (ADS)

McGonigle, Lorcan; Choi, P. I.; Severson, S. A.; Spjut, E.

2013-01-01

KAPAO (KAPAO A Pomona Adaptive Optics instrument) is a dual-band natural guide star adaptive optics system designed to measure and remove atmospheric aberration over UV-NIR wavelengths from Pomona College’s telescope atop Table Mountain. We present here, the final optical system, KAPAO Prime, designed in Zemax Optical Design Software that uses custom off-axis paraboloid mirrors (OAPs) to manipulate light appropriately for a Shack-Hartman wavefront sensor, deformable mirror, and science cameras. KAPAO Prime is characterized by diffraction limited imaging over the full 81” field of view of our optical camera at f/33 as well as over the smaller field of view of our NIR camera at f/50. In Zemax, tolerances of 1% on OAP focal length and off-axis distance were shown to contribute an additional 4 nm of wavefront error (98% confidence) over the field of view of our optical camera; the contribution from surface irregularity was determined analytically to be 40nm for OAPs specified to λ/10 surface irregularity (632.8nm). Modeling of the temperature deformation of the breadboard in SolidWorks revealed 70 micron contractions along the edges of the board for a decrease of 75°F when applied to OAP positions such displacements from the optimal layout are predicted to contribute an additional 20 nanometers of wavefront error. Flexure modeling of the breadboard due to gravity is on-going. We hope to begin alignment and testing of KAPAO Prime in Q1 2013.

Immunological inquiry for the epidemiology of leptospirosis in Canis familiaris in Salvador, Bahia, Brazil.

PubMed

Caldas, E M; Doria, J D; Martins, M A

1977-12-01

A total of 430 dogs were immunologically examined in Salvador, Bahia, Brazil, and 21.6% of them were positive. The animals were selected through an aleatory pattern, according to the division of the City in 18 residential zones a division which had been established by the vaccination campaign against canis hydrophobia. The serotypes icterohemorrhagiae and canicola were the most frequently registered. Between the sexes, the male presented the highest index of positivity, though the difference wasn't statistically significant. The period of age ranging from 12 to 48 months comprised 67.7% of the investigations. Mestizo dogs presented a higher frequency of positivity than any other race and the areas of Nordeste de Amaralina, Liberdade and Quintas showed the greatest percentages of positive dogs.

[Health among persons with intellectual disability in Spain: the European POMONA-II study].

PubMed

Martínez-Leal, Rafael; Salvador-Carulla, Luis; Gutiérrez-Colosía, Mencía Ruiz; Nadal, Margarida; Novell-Alsina, Ramón; Martorell, Almudena; González-Gordón, Rodrigo G; Mérida-Gutiérrez, M Reyes; Ángel, Silvia; Milagrosa-Tejonero, Luisa; Rodríguez, Alicia; García-Gutiérrez, Juan C; Pérez-Vicente, Amado; García-Ibáñez, José; Aguilera-Inés, Francisco

2011-10-01

International studies show that both the pattern of health and the healthcare provided for persons with intellectual disability (ID) and the general population are different. To obtain data about the state of health of persons with ID and to compare them with data about the general population. The P15 set of health indicators was used in a sample of 111 subjects with ID. The health data that were found were compared according to the subjects' type of residence and the 2006 National Health Survey was used to compare these data with those for the general population. RESULTS; The sample with ID presented 25 times more cases of epilepsy and twice as many cases of obesity. Twenty per cent presented pain in the mouth and the presence of sensory and mobility problems, as well as psychosis, was high. We also found, however, a low presence of pathologies like diabetes, hypertension, osteoarthritis and osteoporosis. They also displayed a lower rate of participation in prevention and health promotion programmes, a higher number of hospital admissions and a lower usage of emergency services. The pattern of health of persons with ID differs from that of the general population, and they use healthcare services differently. It is important to develop programmes of health promotion and professional training that are specifically designed to attend to the needs of persons with ID. Likewise, it is also necessary to implement health surveys that include data about this population.

Studies to Control Endemic Typhoid Fever in Chile

DTIC Science & Technology

1982-01-29

de las colecistopatias en Chile . Rev.Med.Chile 100:1376- 1381, 1972. 8. Medina, E., Kaempffer, A.M., DeCroizet, V.A., Yrrazaval, Toporowicz, M...Epidemoologia de las colecistopatias en Chile . II. Factores de importancia en estudios de autopsia. Rev.Med.Chile 100:1382-1389, 1972. 9. Marinovio, I...necesario ef ec tuar previamente ex5menes bactereol.’gicos de inocuidad y potencia_ de la vacuna en el Instituto de Salud Priblica de

The California-Arizona Minority Partnership for Astronomy Research and Education (CAMPARE): an Educational Experience for Undergraduates at the University of Arizona Alumni Association's Astronomy Camp.

NASA Astrophysics Data System (ADS)

Lemon, Courtney; McCarthy, D.; Rudolph, A.

2011-01-01

The California-Arizona Minority Partnership for Astronomy Research and Education (CAMPARE) is an NSF-funded partnership between the Astronomy Program at Cal Poly Pomona (CPP) and the University of Arizona Steward Observatory designed to promote participation of underrepresented minorities (including women) in astronomy research and education. As part of the education component of the program, CPP undergraduate physics majors and minors are eligible to work as a counselor at the University of Arizona's Astronomy Camp, one of the premier astronomy outreach opportunities in the world. CAMPARE students have the opportunity to work in this learn-by-doing environment with a wide range of students to gain first hand experience of teaching astronomy to students of a wide variety of ages in highly structured educational setting. Cal Poly Pomona students who are interested in education, both formal and informal, work in a variety of camps, from Girl Scout camps to camps for advanced high school students, to further their understanding of what it means to be a professional in the field of education. The CAMPARE student who participated in this program during summer 2010 had the opportunity to work under Dr. Don McCarthy, camp director of University of Arizona's Astronomy Camps for 20 years, and observe the interpersonal relations between campers and staff that is so vital to the learning the students receive. Through these observations, the CAMPARE student was able to learn to gauge students' interest in the material, and experience real life teaching and learning scenarios in the informal education realm.

Seroepidemiology of leptospirosis in dogs from rural and slum communities of Los Rios Region, Chile.

PubMed

Lelu, Maud; Muñoz-Zanzi, Claudia; Higgins, Brooke; Galloway, Renee

2015-02-12

Leptospirosis is a zoonotic disease of global importance and often neglected as a public health problem due to lack of awareness, under-diagnosis and under-reporting. Animals serve as a source of transmission through the shedding of Leptospira in their urine. Because of their proximity to humans, dogs may play a role in human infections. In order to assess and mitigate leptospirosis in dogs and the risk of transmission to humans it is important to understand the epidemiology of leptospirosis under natural conditions. This study aimed to characterize leptospirosis in owned dogs from three distinct community types. Blood, dog and household data were collected from 265 dogs in 190 households from 12 communities representing farms, rural villages, and urban slums in the Los Rios region, Chile. Serologic profiles with a 20-serovar microagglutination test panel were obtained. Binomial and multinomial logistic regression models were used to evaluate the associations between spatial, ecological, socio-economic variables and overall seropositivity as well as seropositivity to serogroup Canicola. Results from 247 dogs with no history of vaccination were used. Overall seroprevalence was 25.1% (62/247) with significant differences by community type: 10.9% (9/82) in dogs from farms, 22.3% (21/94) from rural villages, and 45.1% (32/71) from urban slums (p <0.001). This trend by community type was also observed for dogs with evidence of seropositivity to the Canicola serogroup. Factors associated with seropositive dogs included dog density and precipitation two-weeks prior to sampling. Presence of Leptospira positive puddles collected from the peri-domestic household environment was also associated with increased seropositivity. Results suggest that leptospirosis is actively maintained in the dog population in this study region with notably distinct patterns by community type. Dog populations from rural villages, and urban slums in particular, showed evidence of high levels of

Human leptospirosis in Brazil.

PubMed

Corrêa, M O

1975-06-01

Serological data on the prevalence of human leptospiroses in certain regions of Brazil are presented. Out of 467 diseased and clinically healthy persons, 40 were positive in the Amazonia. The most frequent serotypes were grippotyphosa (27.5%), panama (25%), icterohaemorrhagiae (10%) and woffi (10%). In 1966 and 1970, 279 cases were identified in Recife (northeastern Brazil) during outbreaks subsequent to floods. Among these 92.5% belonged to the icterohaemorrhagiae serotype. From 1947 to 1972, in São Paulo City (southeastern Brazil), of 18,233 patients with clinical signs of leptospirosis, 2,237 were positive with 86.5% belonging to icterohaemorrhagiae. In all Brazil, 32 strains of leptospires were isolated, 27 of which belonged to the icterohaemorrhagiae serotype and one strain for each wolffi, canicola, grippotyphosa, andamana and alexi serotypes.

A public-professional web-bridge for vaccines and vaccination: user concerns about vaccine safety.

PubMed

García-Basteiro, Alberto L; Alvarez-Pasquín, María-José; Mena, Guillermo; Llupià, Anna; Aldea, Marta; Sequera, Victor-Guillermo; Sanz, Sergi; Tuells, Jose; Navarro-Alonso, José-Antonio; de Arísteguí, Javier; Bayas, José-María

2012-05-28

Vacunas.org (http://www.vacunas.org), a website founded by the Spanish Association of Vaccinology offers a personalized service called Ask the Expert, which answers any questions posed by the public or health professionals about vaccines and vaccination. The aim of this study was to analyze the factors associated with questions on vaccination safety and determine the characteristics of questioners and the type of question asked during the period 2008-2010. A total of 1341 questions were finally included in the analysis. Of those, 30% were related to vaccine safety. Questions about pregnant women had 5.01 higher odds of asking about safety (95% CI 2.82-8.93) than people not belonging to any risk group. Older questioners (>50 years) were less likely to ask about vaccine safety compared to younger questioners (OR: 0.44, 95% CI 0.25-0.76). Questions made after vaccination or related to influenza (including H1N1) or travel vaccines were also associated with a higher likelihood of asking about vaccine safety. These results identify risk groups (pregnant women), population groups (older people) and some vaccines (travel and influenza vaccines, including H1N1) where greater efforts to provide improved, more-tailored vaccine information in general and on the Internet are required. Copyright © 2011 Elsevier Ltd. All rights reserved.

Cyclical changes in seroprevalence of leptospirosis in California sea lions: endemic and epidemic disease in one host species?

PubMed Central

Lloyd-Smith, James O; Greig, Denise J; Hietala, Sharon; Ghneim, George S; Palmer, Lauren; St Leger, Judy; Grenfell, Bryan T; Gulland, Frances MD

2007-01-01

Background Leptospirosis is a zoonotic disease infecting a broad range of mammalian hosts, and is re-emerging globally. California sea lions (Zalophus californianus) have experienced recurrent outbreaks of leptospirosis since 1970, but it is unknown whether the pathogen persists in the sea lion population or is introduced repeatedly from external reservoirs. Methods We analyzed serum samples collected over an 11-year period from 1344 California sea lions that stranded alive on the California coast, using the microscopic agglutination test (MAT) for antibodies to Leptospira interrogans serovar Pomona. We evaluated seroprevalence among yearlings as a measure of incidence in the population, and characterized antibody persistence times based on temporal changes in the distribution of titer scores. We conducted multinomial logistic regression to determine individual risk factors for seropositivity with high and low titers. Results The serosurvey revealed cyclical patterns in seroprevalence to L. interrogans serovar Pomona, with 4–5 year periodicity and peak seroprevalence above 50%. Seroprevalence in yearling sea lions was an accurate index of exposure among all age classses, and indicated on-going exposure to leptospires in non-outbreak years. Analysis of titer decay rates showed that some individuals probably maintain high titers for more than a year following exposure. Conclusion This study presents results of an unprecedented long-term serosurveillance program in marine mammals. Our results suggest that leptospirosis is endemic in California sea lions, but also causes periodic epidemics of acute disease. The findings call into question the classical dichotomy between maintenance hosts of leptospirosis, which experience chronic but largely asymptomatic infections, and accidental hosts, which suffer acute illness or death as a result of disease spillover from reservoir species. PMID:17986335

Presence of leptospires on genital tract of mares with reproductive problems.

PubMed

Hamond, Camila; Pestana, Cristiane P; Rocha-de-Souza, Cláudio Marcos; Cunha, Luis Eduardo R; Brandão, Felipe Z; Medeiros, Marco Alberto; Lilenbaum, Walter

2015-09-30

Leptospirosis is a zoonotic disease of global importance, and has a worldwide distribution. Equine leptospirosis is commonly manifested by recurrent uveitis, reproductive disorders, as abortions, embryonic absorption, stillbirth and the birth of weak foals. The aim of this study was to verify the presence of Leptospira sp or its DNA in genital tract of mares with reproductive problems. A total of 38 mares with reproductive problems were studied. All the mares were sampled for blood (for serology), urine (for culturing and qPCR), vaginal fluid-VF and endometrial biopsy-EB (for culturing, qPCR and indirect immunofluorescence). PCRs products were sequenced for secY gene. Seventeen (44.7%) serum samples were reactive, predominantly against serogroups Australis (76.4%) and Pomona (23.6%). No positive culture was obtained, but DNA was detected by qPCR on urine samples (26.3%), VF (44.7%) and EB (18.4%) collected 2 months or longer following diagnosis of early fetal death and endometritis. Leptospira cell aggregations were visible by indirect immunofluorescence on 57.1% (4/7) EBs and 17.6% (3/17) VFs. A total of 18 amplicons showed interpretable sequences. Out of those 18 amplicons, 15 presented 100% of identity with the species L. interrogans (sv Bratislava and Pomona), while three were L. borgpertersenii. This study suggests the presence of leptospires in the uterus of mares with reproductive problems. Moreover, serology was shown not to be indicated for the diagnosis of presumptive Leptospira infection in early gestation. The most common agent of the genital infection in those mares was L. interrogans, most probably sg Australis. Copyright © 2015 Elsevier B.V. All rights reserved.

Cal Poly Pomona NUE Project: Implementing Microscale and Nanoscale Investigations Throughout the Undergraduate Curriculum

PubMed Central

Vandervoort, Kurt; Brelles-Mariño, Graciela

2013-01-01

NUE funded work at California State Polytechnic University involved development and implementation of nanotechnology modules for physics courses spanning all levels of the undergraduate curriculum, from freshman service courses to senior level laboratories and independent research projects. These modules demonstrate the application of fundamental physics at the nanoscale that complement macroscopic investigations. The introductory level and some of the advanced level modules have been described previously in journal papers and will be outlined briefly here. The main focus of this article, however, is to describe some newer work involving nanoscale experiments that have been developed for senior level laboratories and independent research. These experiments involve applications as diverse as tunneling diodes, gas discharge plasmas for biofilm inactivation, and quantized conductance in gold nanowires. PMID:24163716

Developing Health Indicators for People with Intellectual Disabilities. The Method of the Pomona Project

ERIC Educational Resources Information Center

van Schrojenstein Lantman-de Valk, H.; Linehan, C.; Kerr, M.; Noonan-Walsh, P.

2007-01-01

Aim: Recently, attention has focused on the health inequalities experienced by people with intellectual disabilities (ID) when compared with the general population. To inform policies aimed at equalizing health opportunities, comparable evidence is needed about the aspects of their health that may be amenable to intervention. Method: Applying the…

Cal Poly Pomona NUE Project: Implementing Microscale and Nanoscale Investigations Throughout the Undergraduate Curriculum.

PubMed

Vandervoort, Kurt; Brelles-Mariño, Graciela

2013-06-01

NUE funded work at California State Polytechnic University involved development and implementation of nanotechnology modules for physics courses spanning all levels of the undergraduate curriculum, from freshman service courses to senior level laboratories and independent research projects. These modules demonstrate the application of fundamental physics at the nanoscale that complement macroscopic investigations. The introductory level and some of the advanced level modules have been described previously in journal papers and will be outlined briefly here. The main focus of this article, however, is to describe some newer work involving nanoscale experiments that have been developed for senior level laboratories and independent research. These experiments involve applications as diverse as tunneling diodes, gas discharge plasmas for biofilm inactivation, and quantized conductance in gold nanowires.

Detection of Leptospira spp. and Brucella abortus antibodies in free-living jaguars (Panthera onca) in two protected areas of northern Pantanal, Brazil.

PubMed

Onuma, Selma Samiko Miyazaki; Kantek, Daniel Luis Zanella; Crawshaw Júnior, Peter Gransden; Morato, Ronaldo Gonçalves; May-Júnior, Joares Adenilson; Morais, Zenaide Maria de; Ferreira Neto, José Soares; Aguiar, Daniel Moura de

2015-01-01

This study aimed to assess the exposure of free-living jaguars (Panthera onca) to Leptospira spp. and Brucella abortus in two conservation units in the Pantanal of Mato Grosso, Brazil. The presence of antibodies in blood samples of eleven jaguars was investigated using autochthonous antigens isolated in Brazil added to reference antigen collection applied to diagnosis of leptospirosis by Microscopic Agglutination Test (MAT). The Rose Bengal test was applied for B. abortus antibodies. Two (18.2%) jaguars were seroreactive for the Leptospira spp. antigen and the serovar considered as most infective in both animals was a Brazilian isolate of serovar Canicola (L01). All jaguars were seronegative for B. abortus. These data indicate that the inclusion of autochthonous antigens in serological studies can significantly increase the number of reactive animals, as well as modify the epidemiological profile of Leptospira spp. infection.

Fall 1991 Ocean Sciences Student Papers

NASA Astrophysics Data System (ADS)

1992-04-01

Michele Okihiro received an Outstanding Student Paper Award for a paper she presented at the AGU Fall 1991 Meeting entitled “Infragravity Bound Waves in Shallow and Deep Water.” Okihiro received a Bachelor of Arts degree in mathematics from Pomona College in 1980, a Bachelor of Science degree in civil engineering from the University of Hawaii in 1988, and a Master of Science degree in oceanography from the University of California at San Diego in 1986. Okihiro is currently working toward her doctorate in oceanography at the University of California at San Diego. Her research at Scripps Institution concerns infragravity waves and their role in forcing resonant harbor oscillations.

PATHOGENIC LEPTOSPIRA SEROVARS IN FREE-LIVING SEA LIONS IN THE GULF OF CALIFORNIA AND ALONG THE BAJA CALIFORNIA COAST OF MEXICO.

PubMed

Avalos-Téllez, Rosalía; Carrillo-Casas, Erika M; Atilano-López, Daniel; Godínez-Reyes, Carlos R; Díaz-Aparicio, Efrén; Ramírez-Delgado, David; Ramírez-Echenique, María F; Leyva-Leyva, Margarita; Suzán, Gerardo; Suárez-Güemes, Francisco

2016-04-28

The California sea lion ( Zalophus californianus ), a permanent inhabitant of the Gulf of California in Mexico, is susceptible to pathogenic Leptospira spp. infection, which can result in hepatic and renal damage and may lead to renal failure and death. During summer 2013, we used the microscopic agglutination test (MAT) to investigate the prevalence of anti-Leptospira antibodies in blood of clinically healthy sea lion pups from seven rookery islands on the Pacific Coast of Baja California (Pacific Ocean) and in the Gulf of California. We also used PCR to examine blood for Leptospira DNA. Isolation of Leptospira in liquid media was unsuccessful. We found higher antibody prevalence in sea lions from the rookery islands in the gulf than in those from the Pacific Coast. Antibodies against 11 serovars were identified in the Gulf of California population; the most frequent reactions were against serovars Bataviae (90%), Pyrogenes (86%), Wolffi (86%), Celledoni (71%), and Pomona (65%). In the Pacific Ocean population, MAT was positive against eight serovars, where Wolffi (88%), Pomona (75%), and Bataviae (70%) were the most frequent. Serum samples agglutinated with more than one Leptospira serovar. The maximum titer was 3,200. Each island had a different serology profile, and islands combined showed a distinct profile for each region. We detected pathogenic Leptospira DNA in 63% of blood samples, but we found no saprophytic Leptospira. Positive PCR results were obtained in blood samples with high and low MAT titers. Together, these two methods enhance the diagnosis and interpretation of sea lion leptospirosis. Our results may be related to human activities or the presence of other reservoirs with which sea lions interact, and they may also be related to sea lion stranding.

DETECTION OF Leptospira spp. AND Brucella abortus ANTIBODIES IN FREE-LIVING JAGUARS (Panthera onca) IN TWO PROTECTED AREAS OF NORTHERN PANTANAL, BRAZIL

PubMed Central

ONUMA, Selma Samiko Miyazaki; KANTEK, Daniel Luis Zanella; CRAWSHAW, Peter Gransden; MORATO, Ronaldo Gonçalves; MAY-JÚNIOR, Joares Adenilson; de MORAIS, Zenaide Maria; FERREIRA, José Soares; de AGUIAR, Daniel Moura

2015-01-01

 This study aimed to assess the exposure of free-living jaguars (Panthera onca) to Leptospira spp. and Brucella abortus in two conservation units in the Pantanal of Mato Grosso, Brazil. The presence of antibodies in blood samples of eleven jaguars was investigated using autochthonous antigens isolated in Brazil added to reference antigen collection applied to diagnosis of leptospirosis by Microscopic Agglutination Test (MAT). The Rose Bengal test was applied for B. abortus antibodies. Two (18.2%) jaguars were seroreactive for the Leptospira spp. antigen and the serovar considered as most infective in both animals was a Brazilian isolate of serovar Canicola (L01). All jaguars were seronegative for B. abortus. These data indicate that the inclusion of autochthonous antigens in serological studies can significantly increase the number of reactive animals, as well as modify the epidemiological profile of Leptospira spp. infection. PMID:25923900

Prevalence of leptospirosis in dairy cattle from small rural production units in Toluca Valley, State of Mexico.

PubMed

Leon, L L; Garcia, R C; Diaz, C O; Valdez, R B; Carmona, G C A; Velazquez, B L G

2008-12-01

In order to know the seroprevalence of Leptospira spp. in stabled dairy cattle, a study was conducted from 2004 to 2006 in which 416 sera were tested using a microscopic agglutination test conducted on microplates. A collection of culture reference antigens, each representing a serogroup, was used for these tests. Results showed that 10.33% (43) of the animals had antibody titers ranging from 1:100 to 1:1600. The main serovars detected in these tests were L. interrogans serovar hardjo and L. interrogans serovar canicola. It is important to note that these serovars represent a high risk for transmission to other susceptible animal species, between individuals, and to human health. This serological survey provides useful information establishing the presence or absence of these serovars in this type of herd. The range of antigens used in this study included serovars representative of all common serogroups.

La salud en personas con discapacidad intelectual en España: estudio europeo POMONA-II

PubMed Central

Martínez-Leal, Rafael; Salvador-Carulla, Luis; Gutiérrez-Colosía, Mencía Ruiz; Nadal, Margarida; Novell-Alsina, Ramón; Martorell, Almudena; González-Gordón, Rodrigo G.; Mérida-Gutiérrez, M. Reyes; Ángel, Silvia; Milagrosa-Tejonero, Luisa; Rodríguez, Alicia; García-Gutiérrez, Juan C.; Pérez-Vicente, Amado; García-Ibáñez, José; Aguilera-Inés, Francisco

2011-01-01

Introducción Estudios internacionales demuestran que existe un patrón diferenciado de salud y una disparidad en la atención sanitaria entre personas con discapacidad intelectual (DI) y población general. Objetivo Obtener datos sobre el estado de salud de las personas con DI y compararlos con datos de población general. Pacientes y métodos Se utilizó el conjunto de indicadores de salud P15 en una muestra de 111 sujetos con DI. Los datos de salud encontrados se compararon según el tipo de residencia de los sujetos y se utilizó la Encuesta Nacional de Salud 2006 para comparar estos datos con los de la población general. Resultados La muestra con DI presentó 25 veces más casos de epilepsia y el doble de obesidad. Un 20% presentó dolor bucal, y existió una alta presencia de problemas sensoriales, de movilidad y psicosis. Sin embargo, encontramos una baja presencia de patologías como la diabetes, la hipertensión, la osteoartritis y la osteoporosis. También presentaron una menor participación en programas de prevención y promoción de la salud, un mayor número de ingresos hospitalarios y un uso menor de los servicios de urgencia. Conclusiones El patrón de salud de las personas con DI difiere del de la población general, y éstas realizan un uso distinto de los servicios sanitarios. Es importante el desarrollo de programas de promoción de salud y de formación profesional específicamente diseñados para la atención de personas con DI, así como la implementación de encuestas de salud que incluyan datos sobre esta población. PMID:21948011

Asymptomatic and chronic carriage of Leptospira interrogans serovar Pomona in California sea lions (Zalophus californianus)

USDA-ARS?s Scientific Manuscript database

Since 1970, periodic outbreaks of leptospirosis, caused by pathogenic spirochetes in the genus Leptospira, have caused morbidity and mortality of California sea lions (Zalophus californianus) along the Pacific coast of North America. Yearly seasonal epizootics of varying magnitude occur between the ...

Polymerase chain reaction for detection of Leptospira spp. in clinical samples.

PubMed Central

Mérien, F; Amouriaux, P; Perolat, P; Baranton, G; Saint Girons, I

1992-01-01

A sensitive assay for Leptospira spp., the causative agent of leptospirosis, was developed on the basis of the polymerase chain reaction (PCR). A 331-bp sequence from the Leptospira interrogans serovar canicola rrs (16S) gene was amplified, and the PCR products were analyzed by DNA-DNA hybridization by using a 289-bp fragment internal to the amplified DNA. Specific PCR products also were obtained with DNA from the closely related nonpathogenic Leptospira biflexa but not with DNA from other spirochetes, such as Borrelia burgdorferi, Borrelia hermsii, Treponema denticola, Treponema pallidum, Spirochaeta aurantia, or more distant organisms such as Escherichia coli, Staphylococcus aureus, Mycobacterium tuberculosis, and Proteus mirabilis. The assay was able to detect as few as 10 bacteria. Leptospira DNA was detected in urine from experimentally infected mice. In addition, the test was found to be suitable for diagnosing leptospirosis in humans. Cerebrospinal fluid and urine from patients with leptospirosis were positive, whereas samples from control uninfected patients were negative. Images PMID:1400983

Role of sph2 Gene Regulation in Hemolytic and Sphingomyelinase Activities Produced by Leptospira interrogans.

PubMed

Narayanavari, Suneel A; Lourdault, Kristel; Sritharan, Manjula; Haake, David A; Matsunaga, James

2015-01-01

Pathogenic members of the genus Leptospira are the causative agents of leptospirosis, a neglected disease of public and veterinary health concern. Leptospirosis is a systemic disease that in its severest forms leads to renal insufficiency, hepatic dysfunction, and pulmonary failure. Many strains of Leptospira produce hemolytic and sphingomyelinase activities, and a number of candidate leptospiral hemolysins have been identified based on sequence similarity to well-characterized bacterial hemolysins. Five of the putative hemolysins are sphingomyelinase paralogs. Although recombinant forms of the sphingomyelinase Sph2 and other hemolysins lyse erythrocytes, none have been demonstrated to contribute to the hemolytic activity secreted by leptospiral cells. In this study, we examined the regulation of sph2 and its relationship to hemolytic and sphingomyelinase activities produced by several L. interrogans strains cultivated under the osmotic conditions found in the mammalian host. The sph2 gene was poorly expressed when the Fiocruz L1-130 (serovar Copenhageni), 56601 (sv. Lai), and L495 (sv. Manilae) strains were cultivated in the standard culture medium EMJH. Raising EMJH osmolarity to physiological levels with sodium chloride enhanced Sph2 production in all three strains. In addition, the Pomona subtype kennewicki strain LC82-25 produced substantially greater amounts of Sph2 during standard EMJH growth than the other strains, and sph2 expression increased further by addition of salt. When 10% rat serum was present in EMJH along with the sodium chloride supplement, Sph2 production increased further in all strains. Osmotic regulation and differences in basal Sph2 production in the Manilae L495 and Pomona strains correlated with the levels of secreted hemolysin and sphingomyelinase activities. Finally, a transposon insertion in sph2 dramatically reduced hemolytic and sphingomyelinase activities during incubation of L. interrogans at physiologic osmolarity

Herd- and animal-level risk factors for bovine leptospirosis in Tanga region of Tanzania.

PubMed

Schoonman, L; Swai, Emanuel Senyael

2010-10-01

Leptospirosis is the zoonosis of worldwide distribution and common cause of economic loss and ill health among animals and human populations. A cross-sectional seroprevalence study, using a microscopic agglutination test (MAT) with a threshold titre of >or=1:160, to elucidate disease magnitude, distribution and associated risk factors in cattle in Tanga, Tanzania was conducted from May 2003 to January 2004. Serum (n = 655) samples collected from randomly selected herds (n = 130) were tested for antibodies against four different Leptospira interrogans serovars (Bataviae, Tarassovi, Hardjo and Pomona) used in the agglutination test. Positive titres were detected in 30.3% [95% confidence intervals (CI) = 26.7-33.9] of cattle and 58.5% (95% CI = 49.5-67.1) of herds, respectively. Of the 198 MAT positive serum samples, 98 (49.5%) were positive against serovar Hardjo, 80 (40.4%) were positive against serovar Tarassovi, 12 (6.1%) was positive against serovar Bataviae and eight (4%) were positive against serovar Pomona. Associations found to be statistically significant in univariate analyses (at P < 0.1) were assessed by multivariable logistic regression to control for confounding factors. The results showed that risk factors for cattle were pasture grazing [odd ratio (OR) = 2.83, 95% CI = 1.57-5.12, P = 0.001], presence of goats/sheep on the farm (OR = 1.73, 95% CI = 1.17-2.56, P = 0.001) and age of the animal (OR = 2.05, 95% CI = 1.42-2.96, P = 0.001), while concrete floor housing was protective (OR = 0.47, 95% CI = 0.30-0.74, P = 0.001). Herds managed under pasture grazing system were more likely to be sero-positive than those managed under zero grazed practices (OR = 9.31; 95% CI = 3.67-23.64 for grazing herd). We concluded that bovine leptospirosis is an endemic and locally widespread disease in Tanga and suggest that it may play a role in zoonotic transmission to humans.

Shedding and seroprevalence of pathogenic Leptospira spp. in sheep and cattle at a New Zealand Abattoir.

PubMed

Fang, F; Collins-Emerson, J M; Cullum, A; Heuer, C; Wilson, P R; Benschop, J

2015-06-01

A cross-sectional study was carried out on sheep and cattle slaughtered at a New Zealand abattoir from September to November 2010 to investigate the supplier-specific shedding rate, renal carriage rate and seroprevalence of leptospires. In the 2008/2009 season, this abattoir experienced three human leptospirosis cases from 20 staff, of which two were hospitalized. Urine, kidney and blood samples were collected from carcasses of 399 sheep (six suppliers, 17 slaughter lines) and 146 cattle (three suppliers, 22 slaughter lines). The urine and kidney samples were tested by quantitative real-time PCR (qPCR), while serum samples (from coagulated blood samples) were tested by microscopic agglutination test (MAT). In total, 27% (73/274; 95% CI: 18-37) of urine samples tested positive by qPCR. Species-specific shedding rates (prevalence of positive urine qPCR) were 31% (95% CI: 17-48) for sheep and 21% (95% CI: 14-30) for cattle. For 545 kidney samples tested, 145 were qPCR positive (27%; 95% CI: 17-39). The average prevalence of kidney qPCR positivity was 29% (95% CI: 17-45) for sheep and 21% (95% CI: 15-28) for cattle. Three hundred and thirty of 542 sampled sheep and cattle had antibodies against Leptospira borgpetersenii serovar Hardjobovis (Hardjobovis) and/or Leptospira interrogans serovar Pomona (Pomona), based on reciprocal MAT titre ≥1 : 48 (overall seroprevalence of 61%; 95% CI: 48-73). Seroprevalence was 57% (95% CI: 40-72) for sheep and 73% (95% CI: 59-83) for cattle. Among the seropositive animals, 41% (70/170; 95% CI: 30-54) were shedding (tested positive by urine qPCR) and 42% (137/330; 95% CI: 30-54) had renal carriage (tested positive by kidney qPCR). Some risk management options for abattoirs or farms to prevent human leptospirosis infections include vaccination of maintenance hosts, the use of personal protective equipment, and the application of urine qPCR to detect shedding status of stock as surveillance and as an alert. © 2014 Blackwell Verlag

Launching and Undergraduate Earth System Science Curriculum with a Focus on Global Sustainability: the Loma Linda University Experience

NASA Astrophysics Data System (ADS)

Ford, R. E.; Dunbar, S. G.; Soret, S.; Wiafe, S.; Gonzalez, D.; Rossi, T.

2004-12-01

The vision of the School of Science and Technology (SST) at Loma Linda University (LLU) is to develop an interdisciplinary approach to doing science that bridges the social, biological, earth, and health sciences. It will provide opportunities for undergraduate, graduate, and professional students to apply new tools and concepts to the promotion of global service and citizenship while addressing issues of global poverty, health and disease, environmental degradation, poverty, and social inequality. A primary teaching strategy will be to involve students with faculty in applied field social and science policy research on "global sustainability" issues and problems in real places such as Fiji, Jamaica, Honduras, Bahamas, East Africa, and the US southwest (Great Basin, Salton Sea, coastal California, southern Utah). Recently we became a partner in the NASA/USRA ESSE21 Project (Earth System Science Education for the 21st Century). We bring to that consortium strengths and experience in areas such as social policy, sustainable development, medicine, environmental health, disaster mitigation, humanitarian relief, geoinformatics and bioinformatics. This can benefit ESSE21, the NASA Earth Enterprise Mission, and the wider geosciences education community by demonstrating the relevance of such tools, and methods outside the geosciences. Many of the graduate and undergraduate students who will participate in the new program come from around the world while many others represent underserved populations in the United States. The PI and Co-PIs have strong global as well as domestic experience serving underrepresented communities, e.g. Seth Wiafe from Ghana, Sam Soret from Spain, Stephen Dunbar from the South Pacific, and Robert Ford from Latin America and Africa. Our partnership in implementation will include other institutions such as: La Sierra University, the California State University, Pomona, Center for Geographic Information Science Research, ESRI, Inc., the University of

Characterization of Salmonella Occurring at High Prevalence in a Population of the Land Iguana Conolophus subcristatus in Galápagos Islands, Ecuador

PubMed Central

Franco, Alessia; Hendriksen, Rene S.; Lorenzetti, Serena; Onorati, Roberta; Gentile, Gabriele; Dell'Omo, Giacomo; Aarestrup, Frank M.; Battisti, Antonio

2011-01-01

The aim of the study was to elucidate the association between the zoonotic pathogen Salmonella and a population of land iguana, Colonophus subcristatus, endemic to Galápagos Islands in Ecuador. We assessed the presence of Salmonella subspecies and serovars and estimated the prevalence of the pathogen in that population. Additionally, we investigated the genetic relatedness among isolates and serovars utilising pulsed field gel electrophoresis (PFGE) on XbaI-digested DNA and determined the antimicrobial susceptibility to a panel of antimicrobials. The study was carried out by sampling cloacal swabs from animals (n = 63) in their natural environment on in the island of Santa Cruz. A high prevalence (62/63, 98.4%) was observed with heterogeneity of Salmonella subspecies and serovars, all known to be associated with reptiles and with reptile-associated salomonellosis in humans. Serotyping revealed 14 different serovars among four Salmonella enterica subspecies: S. enterica subsp. enterica (n = 48), S. enterica subsp. salamae (n = 2), S. enterica subsp. diarizonae (n = 1), and S. enterica subsp. houtenae (n = 7). Four serovars were predominant: S. Poona (n = 18), S. Pomona (n = 10), S. Abaetetuba (n = 8), and S.Newport (n = 5). The S. Poona isolates revealed nine unique XbaI PFGE patterns, with 15 isolates showing a similarity of 70%. Nine S. Pomona isolates had a similarity of 84%. One main cluster with seven (88%) indistinguishable isolates of S. Abaetetuba was observed. All the Salmonella isolates were pan-susceptible to antimicrobials representative of the most relevant therapeutic classes. The high prevalence and absence of clinical signs suggest a natural interaction of the different Salmonella serovars with the host species. The interaction may have been established before any possible exposure of the iguanas and the biocenosis to direct or indirect environmental factors influenced by the use of antimicrobials in agriculture

Characterization of Salmonella occurring at high prevalence in a population of the land iguana Conolophus subcristatus in Galápagos Islands, Ecuador.

PubMed

Franco, Alessia; Hendriksen, Rene S; Lorenzetti, Serena; Onorati, Roberta; Gentile, Gabriele; Dell'Omo, Giacomo; Aarestrup, Frank M; Battisti, Antonio

2011-01-01

The aim of the study was to elucidate the association between the zoonotic pathogen Salmonella and a population of land iguana, Colonophus subcristatus, endemic to Galápagos Islands in Ecuador. We assessed the presence of Salmonella subspecies and serovars and estimated the prevalence of the pathogen in that population. Additionally, we investigated the genetic relatedness among isolates and serovars utilising pulsed field gel electrophoresis (PFGE) on XbaI-digested DNA and determined the antimicrobial susceptibility to a panel of antimicrobials. The study was carried out by sampling cloacal swabs from animals (n = 63) in their natural environment on in the island of Santa Cruz. A high prevalence (62/63, 98.4%) was observed with heterogeneity of Salmonella subspecies and serovars, all known to be associated with reptiles and with reptile-associated salomonellosis in humans. Serotyping revealed 14 different serovars among four Salmonella enterica subspecies: S. enterica subsp. enterica (n = 48), S. enterica subsp. salamae (n = 2), S. enterica subsp. diarizonae (n = 1), and S. enterica subsp. houtenae (n = 7). Four serovars were predominant: S. Poona (n = 18), S. Pomona (n = 10), S. Abaetetuba (n = 8), and S. Newport (n = 5). The S. Poona isolates revealed nine unique XbaI PFGE patterns, with 15 isolates showing a similarity of 70%. Nine S. Pomona isolates had a similarity of 84%. One main cluster with seven (88%) indistinguishable isolates of S. Abaetetuba was observed. All the Salmonella isolates were pan-susceptible to antimicrobials representative of the most relevant therapeutic classes. The high prevalence and absence of clinical signs suggest a natural interaction of the different Salmonella serovars with the host species. The interaction may have been established before any possible exposure of the iguanas and the biocenosis to direct or indirect environmental factors influenced by the use of antimicrobials in agriculture

The Spanish human papillomavirus vaccine consensus group: a working model.

PubMed

Cortés-Bordoy, Javier; Martinón-Torres, Federico

2010-08-01

Successful implementation of Human Papillomavirus (HPV) vaccine in each country can only be achieved from a complementary and synergistic perspective, integrating all the different points of view of the diverse related professionals. It is this context where the Spanish HPV Vaccine Consensus Group (Grupo Español de Consenso sobre la Vacuna VPH, GEC-VPH) was created. GEC-VPH philosophy, objectives and experience are reported in this article, with particular attention to the management of negative publicity and anti-vaccine groups. Initiatives as GEC-VPH--adapted to each country's particular idiosyncrasies--might help to overcome the existing barriers and to achieve wide and early implementation of HPV vaccination.

First Light of the Renovated Thacher Observatory

NASA Astrophysics Data System (ADS)

O'Neill, Katie; Yin, Yao; Edwards, Nick; Swift, Jonathan

2017-01-01

The Thacher Observatory, originally a collaboration between UCLA (P.I. G. Abell), Caltech, Pomona College, and the Thacher School, was built in the early 1960s. The goal of the facility was to serve as a training ground for undergraduate and graduate students in Los Angeles area colleges and also to provide hands-on technical training and experience for Thacher students. It was the birthplace of the Summer Science Program which continues today at other campuses. The observatory has now been fully renovated and modernized with a new, 0.7m telescope and dome that can be controlled remotely and in an automated manner. Science programs involving accurate and precise photometry have been initiated, and we project that we will be presenting the first scientific results of the renovated observatory at this meeting.

Physics By Inquiry: Addressing Student Learning and Attitude

NASA Astrophysics Data System (ADS)

Sadaghiani, Homeyra R.

2008-10-01

In the last decade, the results of Physics Education Research and research-based instructional materials have been disseminated from traditional research universities to a wide variety of colleges and universities. Nevertheless, the ways in which different institutions implement these materials depend on their students and the institutional context. Even with the widespread use of these curriculums, the research documenting the effectiveness of these materials with different student populations is scarce. This paper describes the challenges associated with implementing Physics by Inquiry at California State Polytechnic University Pomona and confirms its effectiveness in promoting student conceptual knowledge of physics. However, despite the positive effect on student learning, the evidence suggests that the students did not appreciate the self-discovery aspect of the inquiry approach and characterized the learning process as difficult and unpleasant.

Antimicrobial susceptibilities and capsular types of invasive Streptococcus pneumoniae isolated in children in Mexico City.

PubMed

Echániz-Aviles, G; Velázquez-Meza, M E; Carnalla-Barajas, M N; Soto-Noguerón, A; Solórzano-Santos, F; Pérez Miravete, A; Gatica-Marquina, R; di Fabio, J L

1997-01-01

As part of the Sistema Regional de Vacunas (SIREVA) initiative, we conducted a surveillance study to determine the relative prevalence of capsular types of Streptococcus pneumoniae and antimicrobial susceptibility of invasive isolates in children less than 5 years old. We collected 220 isolates and found 33 of the 90 known types, with type 23F as the most common followed by types 6A+B, 14, 19F, and 19A. High penicillin resistance was found in 49 strains (22.2%), 31 belonging to type 23F. Twenty-nine (13.1%) were resistant to erythromycin, 95 (43.1%) were resistant to chloramphenicol, and 24 (10.9%) were resistant to cefotaxime. No strains were resistant to vancomycin.

Scientific Reasoning for Pre-service Elementary Teachers

NASA Astrophysics Data System (ADS)

Sadaghiani, Homeyra R.

2010-10-01

The objectives of K-12 teacher education science courses often focus on conceptual learning and improving students overall attitude towards science. It is often assumed that with the use of research-based curriculum material and more hands on inquiry approaches, without any explicit instruction, student scientific and critical thinking skills would also be enhanced. In the last three years, we have been investigating student scientific and evidence-based reasoning abilities in a K-8 pre-service science course at Cal Poly Pomona. After recognizing student difficulties understanding the elements of scientific reasoning, we have provided explicit feedback using a rubric to assist students to become more rigorous and reflective thinkers; to use appropriate and accurate vocabulary; exercise evidence-base reasoning; and develop skepticism with respect to their own views. We will share the rubric and report on the preliminary results.

KAPAO first light: the design, construction and operation of a low-cost natural guide star adaptive optics system

NASA Astrophysics Data System (ADS)

Severson, Scott A.; Choi, Philip I.; Badham, Katherine E.; Bolger, Dalton; Contreras, Daniel S.; Gilbreth, Blaine N.; Guerrero, Christian; Littleton, Erik; Long, Joseph; McGonigle, Lorcan P.; Morrison, William A.; Ortega, Fernando; Rudy, Alex R.; Wong, Jonathan R.; Spjut, Erik; Baranec, Christoph; Riddle, Reed

2014-07-01

We present the instrument design and first light observations of KAPAO, a natural guide star adaptive optics (AO) system for the Pomona College Table Mountain Observatory (TMO) 1-meter telescope. The KAPAO system has dual science channels with visible and near-infrared cameras, a Shack-Hartmann wavefront sensor, and a commercially available 140-actuator MEMS deformable mirror. The pupil relays are two pairs of custom off-axis parabolas and the control system is based on a version of the Robo-AO control software. The AO system and telescope are remotely operable, and KAPAO is designed to share the Cassegrain focus with the existing TMO polarimeter. We discuss the extensive integration of undergraduate students in the program including the multiple senior theses/capstones and summer assistantships amongst our partner institutions. This material is based upon work supported by the National Science Foundation under Grant No. 0960343.

[Reptile-associated Salmonellosis in a Breastfed Infant].

PubMed

Böhme, H; Fruth, A; Rebmann, F; Sontheimer, D; Rabsch, W

2009-01-01

Although salmonellosis is one of the most common childhood illnesses associated with enteric bacteria, breastfed babies do not often get infected with Salmonella. In such cases it is therefore necessary to look carefully for the source of infection. It is reported an 8-week-old female infant with acute haemorrhagic diarrhoea and fever. With symptomatic therapy and breast-feeding her condition improved without antibiotic therapy. Microbiological diagnosis and subtyping identified infection with Salmonella enterica serovar Pomona. The source of the infection was a bearded dragon ( Pogona SPP.) living in a neighborhood household. Monitoring of the child showed shedding of the bacterium over a nine month period. In all cases of salmonellosis in breastfed babies, the source of infection should be carefully determined. Small numbers of bacteria can cause a symptomatic infection in this age group. Therefore an intensive search of the transmission route is indicated to develop prevention strategies.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fecht, K.R.

Gable Mountain and Gable Butte are two ridges which form the only extensive outcrops of the Columbia River Basalt Group in the central portion of the Pasco Basin. The Saddle Mountains Basalt and two interbedded sedimentary units of the Ellensburg Formation crop out on the ridges. These include, from oldest to youngest, the Asotin Member (oldest), Esquatzel Member, Selah Interbed, Pomona Member, Rattlesnake Ridge Interbed, and Elephant Mountain Member (youngest). A fluvial plain composed of sediments from the Ringold and Hanford (informal) formations surrounds these ridges. The structure of Gable Mountain and Gable Butte is dominated by an east-west-trending majormore » fold and northwest-southeast-trending parasitic folds. Two faults associated with the uplift of these structures were mapped on Gable Mountain. The geomorphic expression of the Gable Mountain-Gable Butte area resulted from the comlex folding and subsequent scouring by post-basalt fluvial systems.« less

Further investigation of g factors for the lead monofluoride ground state

NASA Astrophysics Data System (ADS)

Skripnikov, L. V.; Petrov, A. N.; Titov, A. V.; Mawhorter, R. J.; Baum, A. L.; Sears, T. J.; Grabow, J.-U.

2015-09-01

We report the results of our theoretical study and analysis of earlier experimental data for the g -factor tensor components of the ground 2Π1 /2 state of the free PbF radical. The values were obtained both within the relativistic coupled-cluster method combined with the generalized relativistic effective core potential approach and with our fit of the experimental data from [R. J. Mawhorter, B. S. Murphy, A. L. Baum, T. J. Sears, T. Yang, P. M. Rupasinghe, C. P. McRaven, N. E. Shafer-Ray, L. D. Alphei, and J.-U. Grabow, Phys. Rev. A 84, 022508 (2011), 10.1103/PhysRevA.84.022508; A. L. Baum, B.A. thesis, Pomona College, 2011]. The obtained results agree very well with each other but contradict the previous fit performed in the cited works. Our final prediction for g factors is G∥=0.081 (5 ) ,G⊥=-0.27 (1 ) .

Using multimedia learning modules in a hybrid-online course in electricity and magnetism

NASA Astrophysics Data System (ADS)

Sadaghiani, Homeyra R.

2011-06-01

We have been piloting web-based multimedia learning modules (MLMs), developed by the Physics Education Research Group at the University of Illinois at Urbana Champaign (UIUC), as a “prelecture assignment” in several introductory physics courses at California State Polytechnic University at Pomona. In this study, we report the results from a controlled study utilizing modules on electricity and magnetism as a part of a blended hybrid-online course. We asked students in the experimental section to view the MLMs prior to attending the face-to-face class, and to make sure this would not result in additional instructional time, we reduced the weekly class time by one-third. We found that despite reduced class time, student-learning outcomes were not hindered; in fact, the implementation of the UIUC MLMs resulted in a positive effect on student performance on conceptual tests and classroom discussion questions.

Presence of Antibodies to Leptospira spp. in Black-tailed Prairie Dogs ( Cynomys ludovicianus ) and Beavers ( Castor canadensis ) in Northwestern Mexico.

PubMed

López-Pérez, Andrés M; Carreón-Arroyo, Gerardo; Atilano, Daniel; Vigueras-Galván, Ana L; Valdez, Carlos; Toyos, Daniel; Mendizabal, Daniel; López-Islas, Jonathan; Suzán, Gerardo

2017-10-01

Leptospires are widespread spirochete bacteria that infect mammals, including rodents and humans. We investigated the presence of Leptospira antibodies in two species of rodents from San Pedro River Basin (SPRB) in northwestern Mexico as part of the black-tailed prairie dog ( Cynomys ludovicianus ) monitoring plan and the North American beaver ( Castor canadensis ) reintroduction program. We sampled a total of 26 black-tailed prairie dogs and three beavers during October-November 2015. We detected antibodies against Leptospira spp. by microagglutination test in 12 (46%) prairie dogs and in two (67%) beavers. The antibody titers for seropositive rodents varied from 1:100 to 1:200, but none of the animals showed clinical signs of disease. We found seven Leptospira spp. serogroups (Autumnalis, Australis, Bataviae, Canicola, Celledoni, Grippotyphosa, and Sejroe) circulating in rodent species in SPRB. We did not find any differences between sex and age concerning Leptospira-positive rodents. Our findings suggest the presence of endemic cycles and potential risks of Leptospira infection in both species from SPRB. Although the impact of this infection on threatened species remains unclear, human activities and environmental stress might facilitate the emergence or reemergence of leptospirosis disease as has been reported elsewhere.

Leptospirosis on captive wild animals in Latin America.

PubMed

Vieira, Anahi S; Lilenbaum, Walter

2017-12-01

Leptospirosis is a worldwide bacterial infection, being more prevalent in tropical regions. Human beings, domestic animals and wildlife species are susceptible to a wide variety of serovars. Zoos have a great importance in keeping endangered species, increasing populations and accumulating knowledge on these species. Although some studies describe the occurrence of leptospirosis in captivity animals, a systematic review regarding the infection in these animals in tropical areas has never been conducted. Thus, the objective of this study was to systematically gather and analyze data regarding leptospirosis among captive wild animals in Latin America. A total of 141 species were studied, 93 genus, 44 families and 15 orders from three classes. Median seroprevalence ranged from 0 to 52% and predominant serogroups were Canicola and Icterohaemorrhagiae for mammalian, and Sejroe for reptiles. One important outcome is that, when kept on zoos, wildlife is more frequently infected by incidental strains instead of adapted strains. Preventive measures should consider periodic serosurvey of all animal species in captivity, as well as the use of commercial vaccines to reduce leptospiral infection and its hazards. Copyright © 2017 Elsevier Ltd. All rights reserved.

Further investigation of g factors for the lead monofluoride ground state

DOE PAGES

Skripnikov, L. V.; Petrov, A. N.; Titov, A. V.; ...

2015-09-15

We report the results of our theoretical study and analysis of earlier experimental data for the g-factor tensor components of the ground 2II 1/2 state of the free PbF radical. These values obtained both within the relativistic coupled-cluster method combined with the generalized relativistic effective core potential approach and with our fit of the experimental data from [R. J. Mawhorter, B. S. Murphy, A. L. Baum, T. J. Sears, T. Yang, P. M. Rupasinghe, C. P. McRaven, N. E. Shafer-Ray, L. D. Alphei, and J.-U. Grabow, Phys. Rev. A 84, 022508 (2011); A. L. Baum, B.A. thesis, Pomona College, 2011].more » The obtained results agree very well with each other but contradict the previous fit performed in the cited works. Our final prediction for g factors is G ∥=0.081(5),G ⊥=–0.27(1).« less

Foothill Transit Battery Electric Bus Demonstration Results: Second Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eudy, Leslie; Jeffers, Matthew

This report summarizes results of a battery electric bus (BEB) evaluation at Foothill Transit, located in the San Gabriel and Pomona Valley region of Los Angeles County, California. Foothill Transit is collaborating with the California Air Resources Board and the U.S. Department of Energy's (DOE's) National Renewable Energy Laboratory to evaluate its fleet of Proterra BEBs in revenue service. The focus of this evaluation is to compare performance of the BEBs to that of conventional technology and to track progress over time toward meeting performance targets. This project has also provided an opportunity for DOE to conduct a detailed evaluationmore » of the BEBs and charging infrastructure. This is the second report summarizing the results of the BEB demonstration at Foothill Transit and it provides data on the buses from August 2015 through December 2016. Data are provided on a selection of compressed natural gas buses as a baseline comparison.« less

Near-degeneracy in Excited Vibrational States of 207PbF

NASA Astrophysics Data System (ADS)

Mawhorter, Richard; Nguyen, Alexander; Kim, Yongrak; Biekert, Andreas; Sears, Trevor; Grabow, Jens-Uwe; Kudashov, A. D.; Skripnikov, L. V.; Titov, A. V.; Petrov, A. N.

2017-04-01

High-resolution Fourier transform microwave (FTMW) spectroscopy studies of 207PbF have demonstrated the near-degeneracy of two levels of opposite parity. These have attracted attention for the study of parity violation effects and the variation of fundamental constants using 207PbF. Further theoretical work has improved our detailed understanding of both 207PbF and 208PbF, and furthermore recently indicated that the finely split +/- parity levels grow monotonically closer for higher vibrational states. Our experimental results for v = 0-3 confirm this, and are in excellent agreement with our extended theoretical calculations up to v = 4; both will be presented. TJS acknowledges support from Contract No. DE-SC0012704 with the U.S. Department of Energy, Office of Science, supported by its Division of Chemical Sciences, Geosciences and Biosciences within the Office of Basic Energy Sciences., as do RM, AB, YK, & AN from Pomona College & J-UG from the Deutsche Forschungsgemeinschaft (DFG).

Coordinating and planning for human sexuality education.

PubMed

Jenkins, D M

1981-04-01

The coordinator provides parent education, teacher and staff inservice training, team-teaching experiences, student counseling and a broad range of support activities related to the wider community as well as to the schools involved. The need for a coordinator for programs may depend on size and/or diversity of the school population, professional readiness of teachers and types of programs to be implemented. Despite the advances made in the Pomona program, there continue to be such problems as occasional resistance from special interest groups, administrators and school staff, individual instructors' personal value conflicts, transitional nature of student population and turnover in teacher personnel. A coordinator for sexuality programs is certainly not a total remedy for such problems in all school districts; but as an essential element in the solution of those problems, he/she provides an approach which will help school districts in initiating and implementing programs with a greater degree of success.

Canine viral vaccines at a turning point--a personal perspective.

PubMed

Carmichael, L E

1999-01-01

data on duration of immunity become available. "Are too many kinds of vaccines being promoted for dogs?" Distemper and parvovirus vaccines are essential; canine adenovirus vaccines are recommended since the few cases brought to our attention in recent years have been in unvaccinated dogs. Vaccination against respiratory infections is recommended for most dogs, especially those in kennels, or if they are to be boarded. Need has not been clearly established for coronavirus vaccines; Lyme disease vaccines (see below) are useful in preventing illness in areas where the disease exists, but are unnecessary elsewhere since dogs respond rapidly to appropriate antibiotics; current Leptospira bacterins are without benefit since they contain serovars that fail to protect in most areas (noted below). Lyme disease (LD) was not considered here, but newer recombinant (OspA) vaccines are now available that appear to be safe and effective for at least 1 year and they have not caused vaccine-induced postvaccinal lameness, which has been documented with certain whole-cell Lyme disease bacterins. Lyme disease vaccines should be restricted to dogs in, or entering, endemic areas where infested ticks reside. More than 85% of LD cases occur in the mid-Atlantic and Northeastern States, about 10% in six Midwestern states (Michigan, Minnesota, and Wisconsin), and a smaller percentage in restricted areas of northern California and the Pacific Northwest. Leptospirosis also was not discussed here, but vaccines are commonly reported as a cause of anaphylaxis and current vaccines do not contain the serovars prevalent in most regions. The vast majority of cases diagnosed at the New York State Diagnostic Lab at Cornell are grippotyphosa and pomona serovars and there have been no recent cases caused by canicola or icterohemorrhagiae serovars. Because leptospirosis is an important disease of dogs, there is an urgent need for more research and the development of safer vaccines that contain the prevalent

A conserved region of leptospiral immunoglobulin-like A and B proteins as a DNA vaccine elicits a prophylactic immune response against leptospirosis.

PubMed

Forster, Karine M; Hartwig, Daiane D; Seixas, Fabiana K; Bacelo, Kátia L; Amaral, Marta; Hartleben, Cláudia P; Dellagostin, Odir A

2013-05-01

The leptospiral immunoglobulin-like (Lig) proteins LigA and LigB possess immunoglobulin-like domains with 90-amino-acid repeats and are adhesion molecules involved in pathogenicity. They are conserved in pathogenic Leptospira spp. and thus are of interest for use as serodiagnostic antigens and in recombinant vaccine formulations. The N-terminal amino acid sequences of the LigA and LigB proteins are identical, but the C-terminal sequences vary. In this study, we evaluated the protective potential of five truncated forms of LigA and LigB proteins from Leptospira interrogans serovar Canicola as DNA vaccines using the pTARGET mammalian expression vector. Hamsters immunized with the DNA vaccines were subjected to a heterologous challenge with L. interrogans serovar Copenhageni strain Spool via the intraperitoneal route. Immunization with a DNA vaccine encoding LigBrep resulted in the survival of 5/8 (62.5%) hamsters against lethal infection (P < 0.05). None of the control hamsters or animals immunized with the other vaccine preparations survived. The vaccine induced an IgG antibody response and, additionally, conferred sterilizing immunity in 80% of the surviving animals. Our results indicate that the LigBrep DNA vaccine is a promising candidate for inclusion in a protective leptospiral vaccine.

A Conserved Region of Leptospiral Immunoglobulin-Like A and B Proteins as a DNA Vaccine Elicits a Prophylactic Immune Response against Leptospirosis

PubMed Central

Forster, Karine M.; Hartwig, Daiane D.; Seixas, Fabiana K.; Bacelo, Kátia L.; Amaral, Marta; Hartleben, Cláudia P.

2013-01-01

The leptospiral immunoglobulin-like (Lig) proteins LigA and LigB possess immunoglobulin-like domains with 90-amino-acid repeats and are adhesion molecules involved in pathogenicity. They are conserved in pathogenic Leptospira spp. and thus are of interest for use as serodiagnostic antigens and in recombinant vaccine formulations. The N-terminal amino acid sequences of the LigA and LigB proteins are identical, but the C-terminal sequences vary. In this study, we evaluated the protective potential of five truncated forms of LigA and LigB proteins from Leptospira interrogans serovar Canicola as DNA vaccines using the pTARGET mammalian expression vector. Hamsters immunized with the DNA vaccines were subjected to a heterologous challenge with L. interrogans serovar Copenhageni strain Spool via the intraperitoneal route. Immunization with a DNA vaccine encoding LigBrep resulted in the survival of 5/8 (62.5%) hamsters against lethal infection (P < 0.05). None of the control hamsters or animals immunized with the other vaccine preparations survived. The vaccine induced an IgG antibody response and, additionally, conferred sterilizing immunity in 80% of the surviving animals. Our results indicate that the LigBrep DNA vaccine is a promising candidate for inclusion in a protective leptospiral vaccine. PMID:23486420

Evaluation of a recombinant LigB protein of Leptospira interrogans serovar Canicola in an enzyme-linked immunosorbent assay for the serodiagnosis of bovine leptospirosis.

PubMed

Sankar, Surya; Harshan, Hiron M; Somarajan, S R; Srivastava, S K

2010-06-01

A recombinant leptospiral lipoprotein, LigB, was evaluated for use in the diagnosis of bovine leptospirosis by enzyme-linked immunosorbent assay (rLigB IgG ELISA). The standard reference test (Microscopic agglutination test, MAT) of 200 serum samples from cattle suspected of leptospirosis showed that 95 (47.5%) samples had positive agglutination titres, which ranged from 100 to 1600. In rLigB IgG ELISA, 49% of the samples were positive. Sensitivity of IgG ELISA for 95 bovine sera, which had MAT titres of greater than or equal to 100, were 100%. ELISA showed a specificity of 97.1% with 105 bovine sera, which were negative at a 1:50 dilution in MAT for Leptospira interrogans serovars. The results of ELISA and MAT correspond very good. When analytical specificity of IgG ELISA was evaluated using bovine serum samples from animals showing the serum antibodies to other pathogens, no cross-reaction was observed. Thus the recombinant LigB IgG ELISA can be used instead of the MAT as an aid to the diagnosis of bovine leptospirosis. Copyright 2009. Published by Elsevier India Pvt Ltd.

Towards collaboration between unmanned aerial and ground vehicles for precision agriculture

NASA Astrophysics Data System (ADS)

Bhandari, Subodh; Raheja, Amar; Green, Robert L.; Do, Dat

2017-05-01

This paper presents the work being conducted at Cal Poly Pomona on the collaboration between unmanned aerial and ground vehicles for precision agriculture. The unmanned aerial vehicles (UAVs), equipped with multispectral/hyperspectral cameras and RGB cameras, take images of the crops while flying autonomously. The images are post processed or can be processed onboard. The processed images are used in the detection of unhealthy plants. Aerial data can be used by the UAVs and unmanned ground vehicles (UGVs) for various purposes including care of crops, harvest estimation, etc. The images can also be useful for optimized harvesting by isolating low yielding plants. These vehicles can be operated autonomously with limited or no human intervention, thereby reducing cost and limiting human exposure to agricultural chemicals. The paper discuss the autonomous UAV and UGV platforms used for the research, sensor integration, and experimental testing. Methods for ground truthing the results obtained from the UAVs will be used. The paper will also discuss equipping the UGV with a robotic arm for removing the unhealthy plants and/or weeds.

Antibodies to Various Zoonotic Pathogens Detected in Feral Swine (Sus scrofa) at Abattoirs in Texas, USA.

PubMed

Pedersen, Kerri; Bauer, Nathan E; Rodgers, Sandra; Bazan, Luis R; Mesenbrink, Brian T; Gidlewski, Thomas

2017-08-01

The zoonotic risk posed to employees by slaughtering feral swine (Sus scrofa) at two abattoirs in Texas was assessed by testing feral swine serum samples for exposure to influenza A virus, Leptospira, Trichinella spiralis, and Toxoplasma gondii. Blood was collected from a total of 376 feral swine between the two facilities during six separate collection periods in 2015. Antibodies to one or more serovars of Leptospira were identified in 48.9% of feral swine tested, with Bratislava and Pomona as the most commonly detected serovars, and antibodies to influenza A virus were detected in 14.1% of feral swine. Antibodies to T. gondii and T. spiralis were identified in 9.0 and 3.5%, respectively, of feral swine tested. Our results suggest that abattoir employees should be aware of the potential for exposure to various zoonotic pathogens when slaughtering feral swine, wear appropriate personal protective equipment, and participate in medical monitoring programs to ensure detection and prompt treatment. In addition, consumers of feral swine should cook the meat to the appropriate temperature and wash hands and kitchen surfaces thoroughly after preparing meat.

Detection of Neospora caninum DNA by polymerase chain reaction in bats from Southern China.

PubMed

Wang, Xu; Li, Jianhua; Gong, Pengtao; Li, Xianhe; Zhang, Li; He, Biao; Xu, Lin; Yang, Zhengtao; Liu, Quan; Zhang, Xichen

2018-03-23

Neospora caninum is an intracellular protozoan that infects many domestic and wild animals. Domestic dogs and other canids function as definitive hosts, while other mammals serve as natural intermediate hosts. In the present study, the brain tissues of bats collected in Yunnan Province, Southern China were tested by N. caninum specific-nested PCR, targeting the Nc-5 gene and the internal transcribed spacer 1 (ITS1) region of the ribosomal DNA to determine whether bats could be infected with N. caninum. N. caninum DNA was detected in 1.8% (4/227) of bats, i.e., 1.7% (1/60) in Rousettus leschenaultia, 1.7% (1/58) in Hipposideros pomona, 2.9% (2/69) in Rhinolophus pusillus, and none (0/40) in Myotis daubentoniid. The findings of the present study are only the first indication that bats could serve as an intermediate host, and further studies are necessary to confirm whether bats are involved in the transmission of N. caninum infections. Copyright © 2018 Elsevier B.V. All rights reserved.

Salmonella infection in healthy pet reptiles: Bacteriological isolation and study of some pathogenic characters.

PubMed

Bertelloni, Fabrizio; Chemaly, Marianne; Cerri, Domenico; Gall, Françoise Le; Ebani, Valentina Virginia

2016-06-01

The fecal samples from 213 captive reptiles were examined, and 29 (13.61%) Salmonella enterica isolates were detected: 14/62 (22.58%) from chelonians, 14/135 (10.37%) from saurians, and 1/16 (6.25%) from ophidians. The isolates were distributed among 14 different serotypes: Miami, Ebrie, Hermannsweder, Tiergarten, Tornov, Pomona, Poona, Goteborg, Abaetetube, Nyanza, Kumasi, Typhimurium, 50:b:z6, 9,12:z29:1,5, and a non-motile serotype with antigenic formula 1,4,[5],12:-:-. Salmonella typhimurium and 50:b:z6 isolates showed the spv plasmid virulence genes, responsible of the capability to induce extra-intestinal infections. In some cases, pulsed field gel electrophoresis revealed different profiles for the strains of the same serotypes, showing different origins, whereas a common source of infection was supposed when one pulsotype had been observed for isolates of a serovar. Twenty-seven (93.10%) isolates showed resistance to one or more antibiotics. Ceftazidime was active to all the tested isolates, whereas the highest percentages of strains were no susceptible to tigecycline (93.10%), streptomycin (89.66%), and sulfonamide (86.21%).

Serological Prevalence of Leptospirosis Among Rural Communities in the Rejang Basin, Sarawak, Malaysia.

PubMed

Suut, Lela; Mazlan, Mohamad Nur-Azim; Arif, Mohamad Taha; Yusoff, Haironi; Abdul Rahim, Nor-Aliza; Safii, Razitasham; Suhaili, Mohamad Raili

2016-07-01

Leptospirosis is an important zoonotic disease globally and is endemic in Malaysia. A study was conducted in the Rejang basin of Sarawak from June 2011 to May 2013 to determine the seroprevalence of leptospirosis among the communities and dominant infecting Leptospira serovars. A total of 508 human sera were analyzed using ELISA and the microscopic agglutination test (MAT). The seroprevalence of leptospirosis in the study area was 37.4%, with the highest prevalence in Kapit division. More women were positive for leptospirosis (59.5%), and the mean age of seropositive individuals was 42.2 (SD = 18.7) years. Antibody titers between 1:50 and 1:1600 were reported, and serovars djasiman (22.1%), shermani (13.2%), and pomona (7.9%) predominated, with varied distribution between geographical locations. This study highlighted the endemicity and diversity of existing Leptospira serovars within the community. This information should be communicated to local health personnel and communities at risk, and rapid diagnostic capability should be made available to local health facilities. © 2016 APJPH.

Congratulating the California State Polytechnic University, Pomona men's basketball team for winning the 2010 NCAA Division II Men's Basketball National Championship.

THOMAS, 111th Congress

Rep. Napolitano, Grace F. [D-CA-38

2010-05-06

House - 05/27/2010 Referred to the Subcommittee on Higher Education, Lifelong Learning, and Competitiveness. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

Environmental risk factors associated with leptospirosis among butchers and their associates in Jamaica.

PubMed

Brown, P D; McKenzie, M; Pinnock, M; McGrowder, D

2011-01-01

Leptospirosis, a spirochetal zoonosis, is considered an occupational disease of persons engaged in agriculture, sewage works, forestry, and butchery. To determine the environmental sources and the knowledge, attitude and practices for leptospirosis among butchers and slaughterhouse workers, as well as the seroprevalence of leptospirosis among cattle and pigs presented for slaughter. Using an interviewer administered questionnaire, all 110 butchers and other slaughterhouse workers in the parishes of Kingston and St. Andrew, Jamaica were surveyed. In addition, 179 blood samples from animals presented for slaughter were tested for anti-Leptospira antibodies using the microscopic agglutination test (MAT). Analyses indicated that people with the studied occupations are at risk for developing leptospirosis due to several environmental risk factors that exist in slaughterhouses. Among the risk factors, limited knowledge of the disease and its transmission, lower educational level attained, younger age and unhealthy behaviors (e.g., hand washing and improper or lack of use of personal protective gears), presence of stray dogs and rodents, and inadequate maintenance of physical plants, were found to be important. Of the total number of animal samples tested, 20 (11%) were positive. Canicola and Hardjo (among cattle) and Bratislava (among pigs) were the major seroreactors. Butchers and slaughterhouse workers engaged in animal handling and slaughtering could be frequently exposed to leptospirosis, and hence control strategies targeting at these populations should be implemented.

Colony-level assessment of Brucella and Leptospira in the Guadalupe fur seal, Isla Guadalupe, Mexico.

PubMed

Ziehl-Quirós, E Carolina; García-Aguilar, María C; Mellink, Eric

2017-01-24

The relatively small population size and restricted distribution of the Guadalupe fur seal Arctocephalus townsendi could make it highly vulnerable to infectious diseases. We performed a colony-level assessment in this species of the prevalence and presence of Brucella spp. and Leptospira spp., pathogenic bacteria that have been reported in several pinniped species worldwide. Forty-six serum samples were collected in 2014 from pups at Isla Guadalupe, the only place where the species effectively reproduces. Samples were tested for Brucella using 3 consecutive serological tests, and for Leptospira using the microscopic agglutination test. For each bacterium, a Bayesian approach was used to estimate prevalence to exposure, and an epidemiological model was used to test the null hypothesis that the bacterium was present in the colony. No serum sample tested positive for Brucella, and the statistical analyses concluded that the colony was bacterium-free with a 96.3% confidence level. However, a Brucella surveillance program would be highly recommendable. Twelve samples were positive (titers 1:50) to 1 or more serovars of Leptospira. The prevalence was calculated at 27.1% (95% credible interval: 15.6-40.3%), and the posterior analyses indicated that the colony was not Leptospira-free with a 100% confidence level. Serovars Icterohaemorrhagiae, Canicola, and Bratislava were detected, but only further research can unveil whether they affect the fur seal population.

Self-referencing thermometry in the nanoscale =

NASA Astrophysics Data System (ADS)

Brites, Carlos Antonio Delgado Sousa

Na ultima decada emergiu uma linha de investigacao muito activa em termometros nao invasivos e precisos que possam determinar temperatura a escala nanometrica. Esta investigacao foi fortemente estimulada pelas numerosas solicitacoes da nanotecnologia e da biomedicina, por exemplo. Uma das abordagens mais promissoras propoe o uso de ioes trivalentes de lantanideos que apresenta propriedades fotoluminescentes que dependem da temperatura. Neste trabalho demonstra-se que esta tecnica combina as vantagens de te um limite de deteccao de 0.5 graus com sensibilidade ate 4.5 % K-1. Este termometro molecular pode ser processado em filmes finos ou nanoparticulas, abrindo os campos de aplicacao a diferentes utilizacoes. As nanoparticulas de silica produzidas sao caracterizadas na presenca e na ausencia de ioes lantanideos. Sem o metal, as nanoparticulas de APTES/TEOS demonstram ser luminescentes sob excitacao UV sem necessidade de utilizar qualquer tratamento termico. O rendimento quantico de emissao depende apenas da proporcao dos silanos e pode atingir o valor de 0.15 +/- 0.02. A co-dopagem destas nanoparticulas com Eu3+ e Tb3+ permite obter sondas com resposta raciometrica, com a possibilidade de ajustar a gama de temperaturas de operacao e a sensibilidade, via desenho inteligente da matriz de suporte e dos ligandos de beta-dicetona que estao coordenados ao iao metalico. Quando processados como filmes, este termometro permite o mapeamento de temperaturas com resolucao espacial 1.8 mum. A racionalizacao da dependencia de temperatura e uma ferramenta util para desenvolver termometros que operam em gamas de temperatura especificos (e.g. gama de temperatura fisiologica, 290-340 K) com sensibilidade acima de 0.5 % K-1. A combinacao de esforcos de um grande numero de diversas disciplinas ira previsivelmente permitir o surgimento de termometros moleculares novos e sofisticados, preenchendo os principais requisitos das nanociencias.

Leptospirosis in beef herds from western Canada: serum antibody titers and vaccination practices.

PubMed

Van De Weyer, Leanne M; Hendrick, Steve; Rosengren, Leigh; Waldner, Cheryl L

2011-06-01

One study described the frequency of pre-breeding vaccination for leptospirosis in 205 cow-calf herds from across western Canada and the prevalence of positive Leptospira antibody titers in unvaccinated, weaned calves from 61 of these herds. The percentages of herds vaccinated for leptospirosis were 13.7% in 2001 and 8.4% in 2002. Of 1539 calves examined, 13 (0.8%) had a positive antibody titer for a Leptospira serovar; the most common serovar detected was hardjo. A second study examined the prevalence of positive Leptospira antibody titers during the summer grazing season in 313 vaccinated and 478 unvaccinated cows from 40 cow-calf herds in southern Saskatchewan. Antibody titers for 7 Leptospira serovars were measured during the grazing season. Of the non-vaccinated cows, 9.6% were positive in the spring for serovar pomona, 6.7% for serovar grippotyphosa, and 6.1% for serovar icterohaemorrhagiae; the corresponding percentages for the fall were 5.5%, 3.0%, and 1.3%, respectively. Of 781 vaccinated and unvaccinated cows that were sampled twice, 11.3% of vaccinated cows and 2.3% of unvaccinated cows had increases in Leptospira antibody titers during the grazing season.

A DNA fragment of Leptospira interrogans encodes a protein which shares epitopes with equine cornea.

PubMed

Lucchesi, P M; Parma, A E

1999-11-30

Horses infected with Leptospira interrogans present several clinical disorders, one of them being recurrent uveitis. An antigenic relationship between this bacterium and equine cornea has been described in previous studies. With the aim to make progress on defining the molecular basis and pathogenesis of equine recurrent uveitis, here we describe the cloning of one DNA fragment from a Leptospira interrogans serovar pomona genomic lambda gt11 library. Although there are references of transcription of leptospiral genes in E. coli from their own leptospiral promoters, in this recombinant construction the leptospiral DNA was located under the control of lacZ promoter since no expression could be detected in the absence of IPTG. This clone, isolated by expression screening with polyclonal serum raised against equine corneal proteins, encodes a 90 kDa protein of L. interrogans which crossreacts with equine cornea as proved Western-blotting. Antibodies directed against this leptospiral protein strongly recognised a 66 kDa equine corneal protein, one of those recognised by an anti-equine cornea serum. Our findings suggest that an immune response to 90 kDa protein participates in pathogenesis of equine uveitis.

Serovar prevalence of Leptospira in semirural India and the development of an IgM-based indirect ELISA.

PubMed

Chandy, Sara; Kirubanandhan, Lokeshwaran; Hemavathy, Priya; Khadeeja, Anees Mohammad; Kurian, Siby Jacob; Venkataraman, Krishnan; Mørch, Kristine; Mathai, Dilip; Manoharan, Anand

2017-03-31

Leptospirosis is a major public health problem in India. However, it has been underreported and under-diagnosed due to a lack of awareness of the disease, a functional surveillance system, and appropriate laboratory diagnostic facilities. This multicenter study aimed to understand the Leptospira serovars causing leptospirosis in seven secondary-level hospitals in six states in India. Since early and accurate diagnosis of leptospirosis is one of the challenges faced by clinicians in India due to the poor specificity and sensitivity of commercially available diagnostic systems, an in-house indirect enzyme-linked immunosorbent assay (ELISA) was developed. Genomic DNA from L. interrogans serovar Canicola was used for polymerase chain reaction amplification, cloning, and expression of the lipL32 gene in E. coli to amplify, clone, and express the lipL32 gene. Australis was the common serovar seen at all the study centers. Serovar Icterohaemorrhagiae was seen in samples from Tamil Nadu and Assam. In-house ELISA was standardized using the purified recombinant LipL32 polypeptide and was used to evaluate serum. Subsequently, acute serum samples from leptospirosis patients (n = 60) were screened. Compared to the gold standard, the microscopic agglutination test, sensitivity and specificity of the in-house ELISA was 95% and 90%, respectively. Understanding Leptospira serovars circulating in leptospirosis-endemic areas will help to formulate better vaccines. LipL32-based ELISA may serve as a valuable tool for early diagnosis of leptospirosis.

Sensitive Leptospira DNA detection using tapered optical fiber sensor.

PubMed

Zainuddin, Nurul H; Chee, Hui Y; Ahmad, Muhammad Z; Mahdi, Mohd A; Abu Bakar, Muhammad H; Yaacob, Mohd H

2018-03-23

This paper presents the development of tapered optical fiber sensor to detect a specific Leptospira bacteria DNA. The bacteria causes Leptospirosis, a deadly disease but with common early flu-like symptoms. Optical single mode fiber (SMF) of 125 μm diameter is tapered to produce 12 μm waist diameter and 15 cm length. The novel DNA-based optical fiber sensor is functionalized by incubating the tapered region with sodium hydroxide (NaOH), (3-Aminopropyl) triethoxysilane and glutaraldehyde. Probe DNA is immobilized onto the tapered region and subsequently hybridized by its complementary DNA (cDNA). The transmission spectra of the DNA-based optical fiber sensor are measured in the 1500 to 1600 nm wavelength range. It is discovered that the shift of the wavelength in the SMF sensor is linearly proportional with the increase in the cDNA concentrations from 0.1 to 1.0 nM. The sensitivity of the sensor toward DNA is measured to be 1.2862 nm/nM and able to detect as low as 0.1 fM. The sensor indicates high specificity when only minimal shift is detected for non-cDNA testing. The developed sensor is able to distinguish between actual DNA of Leptospira serovars (Canicola and Copenhageni) against Clostridium difficile (control sample) at very low (femtomolar) target concentrations. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Health assessment and seroepidemiologic survey of potential pathogens in wild Antillean manatees (Trichechus manatus manatus)

USGS Publications Warehouse

Sulzner, Kathryn; Johnson, Christine Kreuder; Bonde, Robert K.; Gomez, Nicole Auil; Powell, James; Nielsen, Klaus; Luttrell, M. Page; Osterhaus, A.D.M.E.; Aguirre, A. Alonso

2012-01-01

The Antillean manatee (Trichechus manatus manatus), a subspecies of the West Indian manatee, inhabits fresh, brackish, and warm coastal waters distributed along the eastern border of Central America, the northern coast of South America, and throughout the Wider Caribbean Region. Threatened primarily by human encroachment, poaching, and habitat degradation, Antillean manatees are listed as endangered by the International Union for the Conservation of Nature. The impact of disease on population viability remains unknown in spite of concerns surrounding the species' ability to rebound from a population crash should an epizootic occur. To gain insight on the baseline health of this subspecies, a total of 191 blood samples were collected opportunistically from wild Antillean manatees in Belize between 1997 and 2009. Hematologic and biochemical reference intervals were established, and antibody prevalence to eight pathogens with zoonotic potential was determined. Age was found to be a significant factor of variation in mean blood values, whereas sex, capture site, and season contributed less to overall differences in parameter values. Negative antibody titers were reported for all pathogens surveyed except for Leptospira bratislava, L. canicola, and L. icterohemorrhagiae, Toxoplasma gondii, and morbillivirus. As part of comprehensive health assessment in manatees from Belize, this study will serve as a benchmark aiding in early disease detection and in the discernment of important epidemiologic patterns in the manatees of this region. Additionally, it will provide some of the initial tools to explore the broader application of manatees as sentinel species of nearshore ecosystem health.

Health assessment and seroepidemiologic survey of potential pathogens in wild Antillean manatees (Trichechus manatus manatus).

PubMed

Sulzner, Kathryn; Kreuder Johnson, Christine; Bonde, Robert K; Auil Gomez, Nicole; Powell, James; Nielsen, Klaus; Luttrell, M Page; Osterhaus, A D M E; Aguirre, A Alonso

2012-01-01

The Antillean manatee (Trichechus manatus manatus), a subspecies of the West Indian manatee, inhabits fresh, brackish, and warm coastal waters distributed along the eastern border of Central America, the northern coast of South America, and throughout the Wider Caribbean Region. Threatened primarily by human encroachment, poaching, and habitat degradation, Antillean manatees are listed as endangered by the International Union for the Conservation of Nature. The impact of disease on population viability remains unknown in spite of concerns surrounding the species' ability to rebound from a population crash should an epizootic occur. To gain insight on the baseline health of this subspecies, a total of 191 blood samples were collected opportunistically from wild Antillean manatees in Belize between 1997 and 2009. Hematologic and biochemical reference intervals were established, and antibody prevalence to eight pathogens with zoonotic potential was determined. Age was found to be a significant factor of variation in mean blood values, whereas sex, capture site, and season contributed less to overall differences in parameter values. Negative antibody titers were reported for all pathogens surveyed except for Leptospira bratislava, L. canicola, and L. icterohemorrhagiae, Toxoplasma gondii, and morbillivirus. As part of comprehensive health assessment in manatees from Belize, this study will serve as a benchmark aiding in early disease detection and in the discernment of important epidemiologic patterns in the manatees of this region. Additionally, it will provide some of the initial tools to explore the broader application of manatees as sentinel species of nearshore ecosystem health.

Correlation of Miocene flows of the Columbia River Basalt Group from the central Columbia River Plateau to the coast of Oregon and Washington

USGS Publications Warehouse

Wells, Ray E.; Simpson, R.W.; Bentley, R.D.; Beeson, Melvin H.; Mangan, Margaret T.; Wright, Thomas L.

1989-01-01

Nearly twenty flows of the Columbia River Basalt Group (CRBG) can be paleomagnetically and chemically correlated westward as far as 500 km from the Columbia Plateau in Washington, through the Columbia Gorge, to the Coast Range of Oregon and Washington. In the Coast Range near Cathlamet, Washington, the CRBG flow stratigraphy includes 10 flows of Grande Ronde Basalt (1 low-MgO R2 flow, 6 low-MgO N2 flows, 3 high-MgO N2 flows), 2 flows of Wanapum Basalt (both flows of Sand Hollow from the Frenchman Springs Member), and the Pomona Member of the Saddle Mountains Basalt. Elsewhere in the Coast Range, additional Grande Ronde Basalt flows, including flows of Winterwater or Umtanum, and additional Wanapum flows, including the flows of Ginkgo, have been reported. Thus at least 18 to 20 CRBG flows reached the coast region. Several of these distal flows have distinctive chemical and magnetic characteristics that are shared by nearby isolated intrusions in Coast Range sedimentary rocks, thus strongly supporting recent suggestions that these intrusions are invasive bodies fed by CRBG flows. Magnetization directions from several flows indicate 16 to 30° of clockwise rotation of the coast with respect to the plateau since middle Miocene time.

Comparision of efficacy of two experimental bovine leptospira vaccines under laboratory and field.

PubMed

Balakrishnan, G; Roy, Parimal

2014-05-15

Two different inactivated vaccines for bovine leptospirosis were prepared with five different Leptospira serovars namely australis, ballum, hardjo, hebdomadis and pomona and adjuvanted with Montanide ISA 206 (Vaccine-I) or adjuvanted with Aluminium hydroxide gel (Vaccine-II) to evaluate the efficacy of the vaccines. The immunogenicity of the vaccines was studied in rabbits under experimental condition and in cattle under field condition for a period of 180 days. Antibody response against five different leptospira serovars ranged from 6.84 log2 to 9.64 log2 (GM-MAT titres) in rabbits at 180 days after vaccination with vaccine I, whereas in vaccine II, the titre value ranged from 5.64 log2 to 7.44 log2. In the case of cattle under field condition, vaccine I showed GM-MAT titres of 6.84 log2 to 7.69 log2 against five different serovars at 150 days following vaccination. Such titres were maintained following vaccination with vaccine II for 120 days only. It is concluded that vaccine I is a better vaccine than vaccine II. However both the vaccines showed high immune response of 5.64 log2 at six months of immunization. Vaccination did not cause any adverse reaction in the vaccinated cattle. Copyright © 2014 Elsevier B.V. All rights reserved.

Human Leptospira Isolates Circulating in Mayotte (Indian Ocean) Have Unique Serological and Molecular Features

PubMed Central

Bourhy, P.; Collet, L.; Lernout, T.; Zinini, F.; Hartskeerl, R. A.; van der Linden, Hans; Thiberge, J. M.; Diancourt, L.; Brisse, S.; Giry, C.; Pettinelli, F.

2012-01-01

Leptospirosis is one of the most widespread zoonoses in the world. However, there is a lack of information on circulating Leptospira strains in remote parts of the world. We describe the serological and molecular features of leptospires isolated from 94 leptospirosis patients in Mayotte, a French department located in the Comoros archipelago, between 2007 and 2010. Multilocus sequence typing identified these isolates as Leptospira interrogans, L. kirschneri, L. borgpetersenii, and members of a previously undefined phylogenetic group. This group, consisting of 15 strains, could represent a novel species. Serological typing revealed that 70% of the isolates belonged to the serogroup complex Mini/Sejroe/Hebdomadis, followed by the serogroups Pyrogenes, Grippotyphosa, and Pomona. However, unambiguous typing at the serovar level was not possible for most of the strains because the isolate could belong to more than one serovar or because serovar and species did not match the original classification. Our results indicate that the serovar and genotype distribution in Mayotte differs from what is observed in other regions, thus suggesting a high degree of diversity of circulating isolates worldwide. These results are essential for the improvement of current diagnostic tools and provide a starting point for a better understanding of the epidemiology of leptospirosis in this area of endemicity. PMID:22162544

Osteopathic approach to implementing and promoting interprofessional education.

PubMed

Mackintosh, Susan E; Adams, Clinton E; Singer-Chang, Gail; Hruby, Raymond J

2011-04-01

Multidisciplinary fragmentation contributes to myriad medical errors and as many as 98,000 patient deaths per year. The Institute of Medicine has proposed steps to improve healthcare delivery, including providing more opportunities for interdisciplinary training. The authors describe the interprofessional education (IPE) program at Western University of Health Sciences (WesternU) in Pomona, California. In 2007, 9 colleges at WesternU-including the College of Osteopathic Medicine of the Pacific-undertook an IPE initiative that resulted in creation of a 3-phase program. Part of the IPE development process involved identifying core competencies that were nontechnical and nonclinical and common to all healthcare professions. The IPE development and implementation process and the identified competencies were analyzed for their relationship to the tenets of osteopathic medicine and the core competencies of osteopathic medical education. Although these tenets and core competencies were not intentionally used in the development process of the WesternU IPE program, the analysis revealed that the major components of the program are congruent with the framework of osteopathic principles and practice. The osteopathic medical profession's founding principles, broad-based perspective, and health-promoting tenets put the profession in a position to emerge as one of the leading forces in IPE.

A new multivalent (DHPPi/L4R) canine combination vaccine prevents infection, shedding and clinical signs following experimental challenge with four Leptospira serovars.

PubMed

Wilson, Stephen; Stirling, Catrina; Thomas, Anne; King, Vickie; Plevová, Edita; Chromá, Ludmila; Siedek, Elisabeth; Illambas, Joanna; Salt, Jeremy; Sture, Gordon

2013-06-28

Although effective vaccines have been developed against the common Leptospira serovars, they are still reported in clinical cases, while others are increasingly prevalent. The results from four challenge studies following vaccination of dogs with a new combination vaccine (DHPPi/L4R) containing inactivated L. serovars, L. canicola, L. icterohaemorrhagiae, L. bratislava and L. grippotyphosa conducted to satisfy the requirements of the European Pharmacopoeia monograph (01/2008:0447), are reported. Six week old dogs received two vaccinations, three weeks apart, and were challenged 25 days later with different isolates of the L. serovars. Clinical observations were recorded, and blood, urine and tissue samples were collected for analysis. Following challenge, non-vaccinated dogs demonstrated various clinical signs, while no vaccinated dogs were affected; significant differences in mean clinical scores were observed. Measurable antibody titres to each Leptospira antigen were seen in vaccinated dogs 21 days following the first vaccination, with further increases in antibody titres observed following challenge with the respective Leptospira strain. Non-vaccinated dogs remained seronegative until challenge. Leptospira were re-isolated from the blood, urine, kidney and liver of all non-vaccinated dogs following challenge. In contrast no vaccinated dogs had Leptospira re-isolated from the same tissues. Significant differences were seen in number of days with positive isolation (blood and urine) and in number of dogs with positive samples (kidney and liver). In conclusion, vaccination of dogs with the new vaccine induces protective immunity 25 days after second vaccination with protection against infection, renal infection and clinical signs following challenge. Copyright © 2013 Elsevier Ltd. All rights reserved.

Health Assessment and Seroepidemiologic Survey of Potential Pathogens in Wild Antillean Manatees (Trichechus manatus manatus)

PubMed Central

Sulzner, Kathryn; Kreuder Johnson, Christine; Bonde, Robert K.; Auil Gomez, Nicole; Powell, James; Nielsen, Klaus; Luttrell, M. Page; Osterhaus, A. D. M. E.; Aguirre, A. Alonso

2012-01-01

The Antillean manatee (Trichechus manatus manatus), a subspecies of the West Indian manatee, inhabits fresh, brackish, and warm coastal waters distributed along the eastern border of Central America, the northern coast of South America, and throughout the Wider Caribbean Region. Threatened primarily by human encroachment, poaching, and habitat degradation, Antillean manatees are listed as endangered by the International Union for the Conservation of Nature. The impact of disease on population viability remains unknown in spite of concerns surrounding the species’ ability to rebound from a population crash should an epizootic occur. To gain insight on the baseline health of this subspecies, a total of 191 blood samples were collected opportunistically from wild Antillean manatees in Belize between 1997 and 2009. Hematologic and biochemical reference intervals were established, and antibody prevalence to eight pathogens with zoonotic potential was determined. Age was found to be a significant factor of variation in mean blood values, whereas sex, capture site, and season contributed less to overall differences in parameter values. Negative antibody titers were reported for all pathogens surveyed except for Leptospira bratislava, L. canicola, and L. icterohemorrhagiae, Toxoplasma gondii, and morbillivirus. As part of comprehensive health assessment in manatees from Belize, this study will serve as a benchmark aiding in early disease detection and in the discernment of important epidemiologic patterns in the manatees of this region. Additionally, it will provide some of the initial tools to explore the broader application of manatees as sentinel species of nearshore ecosystem health. PMID:22984521

Multiple-Purpose Project, Osage River Basin, Hundred and Ten Mile Creek Kansas, Pomona Lake, Operation and Maintenance Manual. Appendix VII. Construction Foundation Report. Revision.

DTIC Science & Technology

1983-10-01

Rock, from required excavation, was utilized in the embankment. Amonium nitrate and 60 percent dynamite were used. No blasting records were kept...AD A135 578 MULTPLE-PURPOSE PROJECT OSAGE RIVER BASIN HUNDRED AND 1;TEN MILE CREEK KAA U) CORPS OF ENGINEERS KANSASOASS DS C C ES C IT UNCLASIFE D ...ADDRESS 12. REPORT DATE Estimates & Specifications Section (MRKED-DE), 1977 Revised October 1983 Design Branch (MRKED- D ), Kansas City District, 13

The Library and the Economic Community; A Market Analysis of Information Needs of Business and Industry in the Communities of Pasadena and Pomona, California.

ERIC Educational Resources Information Center

Meyer, Robert S.; Rostvold, Gerhard N.

This study represents a market analysis of the needs, habits and desires of business firms concerning their acquisition and utilization of the information required in their work in order to improve public library services to business and industry through cooperative library action. The key recommendations were: the public library should be the…

Hematology, Parasitology, and Serology of Free-Ranging Coyotes (Canis latrans) from South Carolina.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miller, Debra, Lee; Schrecengost, Joshua; Merrill, Anita

2009-07-01

ABSTRACT: Blood and feces were collected from 34 adult (19 males, 15 females) and seven juvenile (three males, one female, three not reported) free-ranging coyotes (Canis latrans) on the US Department of Energy’s Savannah River Site (South Carolina, USA). Significant (P,0.05) hematologic differences by sex were noted for red blood cell counts, hemoglobin, and hematocrit. Biochemical differences by sex occurred only for albumen (P,0.05). Twentyone adults were antibody positive for at least one of four viruses: canine adenovirus type 1 (CAV-1; 68%), West Nile virus (WNV; 60%), Eastern equine encephalitis virus (EEEV; 38%), and Canine distemper virus (CDV; 15%). Ofmore » the seven Leptospira serovars tested for, seven (25%) of 28 adults were positive for one or more of five serovars: Pomona, Grippotyphosa, Icterohaemorrhagiae, Bratislava, and Autumnalis. Three (43%) of seven juveniles had seropositivity for a virus, one each for CDV, CAV-1, and WNV. No juveniles were seropositive for EEEV or any of the seven Leptospira serovars. Blood smears of 12 adults were positive for Dirofilaria immitis microfilaria, but blood smears from all juveniles were negative. Parvovirus was identified by electron microscopy from the feces of one adult. Ancylostoma spp., Trichuris spp., and Isospora spp. were observed in fecal samples. These data may aid in understanding the role of coyotes in disease ecology.« less

Tiny Turtles Purchased at Pet Stores are a Potential High Risk for Salmonella Human Infection in the Valencian Region, Eastern Spain.

PubMed

Marin, Clara; Vega, Santiago; Marco-Jiménez, Francisco

2016-07-01

Turtles may be considered unsafe pets, particularly in households with children. This study aimed to assess Salmonella carriage by turtles in pet stores and in private ownership to inform the public of the potential health risk, enabling informed choices around pet selection. During the period between September and October 2013, 24 pet stores and 96 private owners were sampled in the Valencian Region (Eastern Spain). Salmonella identification procedure was based on ISO 6579: 2002 recommendations (Annex D). Salmonella strains were serotyped in accordance with Kauffman-White-Le-Minor technique. The rate of isolation of Salmonella was very high from pet store samples (75.0% ± 8.8%) and moderate for private owners (29.0% ± 4.6%). Serotyping revealed 18 different serotypes among two Salmonella enterica subspecies: S. enterica subsp. enterica and S. enterica subsp. diarizonae. Most frequently isolated serotypes were Salmonella Typhimurium (39.5%, 17/43) and Salmonella Pomona (9.3%, 4/43). Serotypes identified have previously been reported in turtles, and child Salmonella infections associate with pet turtle exposure. The present study clearly demonstrates that turtles in pet stores, as well as in private owners, could be a direct or indirect source of a high risk of human Salmonella infections. In addition, pet stores should advise their customers of the potential risks associated with reptile ownership.

Serosurvey of Smooth Brucella, Leptospira spp. and Toxoplasma gondii in Free-Ranging Jaguars (Panthera onca) and Domestic Animals from Brazil.

PubMed

Furtado, Mariana Malzoni; Gennari, Solange Maria; Ikuta, Cassia Yumi; Jácomo, Anah Tereza de Almeida; de Morais, Zenaide Maria; Pena, Hilda Fátima de Jesus; Porfírio, Grasiela Edith de Oliveira; Silveira, Leandro; Sollmann, Rahel; de Souza, Gisele Oliveira; Tôrres, Natália Mundim; Ferreira Neto, José Soares

2015-01-01

This study investigated the exposure of jaguar populations and domestic animals to smooth Brucella, Leptospira spp. and Toxoplasma gondii in the Cerrado, Pantanal and Amazon biomes of Brazil. Between February 2000 and January 2010, serum samples from 31 jaguars (Panthera onca), 1,245 cattle (Bos taurus), 168 domestic dogs (Canis lupus familiaris) and 29 domestic cats (Felis catus) were collected and analysed by rose bengal test for smooth Brucella, microscopic agglutination test for Leptospira spp. and modified agglutination test for T. gondii. Cattle populations from all sites (9.88%) were exposed to smooth Brucella, but only one jaguar from Cerrado was exposed to this agent. Jaguars captured in the Cerrado (60.0%) and in the Pantanal (45.5%) were seropositive for different serovars of Leptospira spp., cattle (72.18%) and domestic dogs (13.1%) from the three sites and one domestic cat from Pantanal were also seropositive for the agent. The most prevalent serotype of Leptospira spp. identified in jaguars from the Cerrado (Grippotyphosa) and the Pantanal (Pomona) biomes were distinct from those found in the domestic animals sampled. Jaguars (100%), domestic dogs (38.28%) and domestic cats (82.76%) from the three areas were exposed to T. gondii. Our results show that brucellosis and leptospirosis could have been transmitted to jaguars by domestic animals; and jaguars probably play an important role in the maintenance of T. gondii in nature.

Philosophy of Education, 1972-1973. Proceedings of the Annual Meeting of the Far Western Philosophy of Education Society (21st, Pomona, California, December 1-3, 1972).

ERIC Educational Resources Information Center

Jelinek, James J., Ed.

The proceedings of the 21st annual meeting of the Far Western Philosophy of Education Society in 1972 are presented. The proceedings consisted of 20 addresses and responses. Titles include (1) Presidential Address: Competency-Based Education: Consensus Cognoscenti Versus Reconstructio Experientiae; (2) A Role for Philosophy in California and…

Serosurvey of Smooth Brucella, Leptospira spp. and Toxoplasma gondii in Free-Ranging Jaguars (Panthera onca) and Domestic Animals from Brazil

PubMed Central

Furtado, Mariana Malzoni; Gennari, Solange Maria; Ikuta, Cassia Yumi; Jácomo, Anah Tereza de Almeida; de Morais, Zenaide Maria; Pena, Hilda Fátima de Jesus; Porfírio, Grasiela Edith de Oliveira; Silveira, Leandro; Sollmann, Rahel; de Souza, Gisele Oliveira; Tôrres, Natália Mundim; Ferreira Neto, José Soares

2015-01-01

This study investigated the exposure of jaguar populations and domestic animals to smooth Brucella, Leptospira spp. and Toxoplasma gondii in the Cerrado, Pantanal and Amazon biomes of Brazil. Between February 2000 and January 2010, serum samples from 31 jaguars (Panthera onca), 1,245 cattle (Bos taurus), 168 domestic dogs (Canis lupus familiaris) and 29 domestic cats (Felis catus) were collected and analysed by rose bengal test for smooth Brucella, microscopic agglutination test for Leptospira spp. and modified agglutination test for T. gondii. Cattle populations from all sites (9.88%) were exposed to smooth Brucella, but only one jaguar from Cerrado was exposed to this agent. Jaguars captured in the Cerrado (60.0%) and in the Pantanal (45.5%) were seropositive for different serovars of Leptospira spp., cattle (72.18%) and domestic dogs (13.1%) from the three sites and one domestic cat from Pantanal were also seropositive for the agent. The most prevalent serotype of Leptospira spp. identified in jaguars from the Cerrado (Grippotyphosa) and the Pantanal (Pomona) biomes were distinct from those found in the domestic animals sampled. Jaguars (100%), domestic dogs (38.28%) and domestic cats (82.76%) from the three areas were exposed to T. gondii. Our results show that brucellosis and leptospirosis could have been transmitted to jaguars by domestic animals; and jaguars probably play an important role in the maintenance of T. gondii in nature. PMID:26605787

Speaking Out on Gender: Reflections on Women's Advancement in the STEM Disciplines

NASA Astrophysics Data System (ADS)

Wachs, Faye Linda; Nemiro, Jill

Faculty at Cal Poly Pomona initiated a campus-wide study to assess the experiences of women in the STEM (Science, Technology, Engineering, and Math) disciplines and to explore what factors were perceived as critical to advancement by successful women on campus. Focus groups with female faculty and administrators at various stages in their career were conducted to address questions of retention, tenure, promotion, and overall job satisfaction. Workload, work-family conflict, and climate emerge as key factors in faculty satisfaction and attributions of success. Ironically, the type of mentoring relationships and professional development cited as key by senior women were rendered improbable for junior female faculty by increasing workloads and work-family conflict. Gender schemas (Valian, 2004) continue to play a role in the increase in workloads and the type of work women are more likely to be asked to do. Women in departments that recognized and accommodated faculty needs, and included faculty in the decision making process, reported much higher levels of satisfaction and productivity than those in inflexible departments. Understanding these issues is critical to overcoming the effects of discrimination such as the continuing shortage of female faculty, especially at the top ranks. Addressing how gender schemas shape the type of work women do within departments and the relative valuation of that work in the RTP (retention, tenure, promotion) process is critical to creating an institutional climate in which female faculty can succeed.

Achieving Drug and Alcohol Abstinence Among Recently Incarcerated Homeless Women: A Randomized Controlled Trial Comparing Dialectical Behavioral Therapy-Case Management With a Health Promotion Program.

PubMed

Nyamathi, Adeline M; Shin, Sanghyuk S; Smeltzer, Jolene; Salem, Benissa E; Yadav, Kartik; Ekstrand, Maria L; Turner, Susan F; Faucette, Mark

Homeless female ex-offenders (homeless female offenders) exiting jail and prison are at a critical juncture during reentry and transitioning into the community setting. The purpose of the study was to compare the effect of a dialectical behavioral therapy-case management (DBT-CM) program with a health promotion (HP) program on achieving drug and alcohol abstinence among female parolees/probationers residing in the community. We conducted a multicenter parallel randomized controlled trial with 130 female parolees/probationers (aged 19-64 years) residing in the community randomly assigned to either DBT-CM (n = 65) or HP (n = 65). The trial was conducted in four community-based partner sites in Los Angeles and Pomona, California, from February 2015 to November 2016. Treatment assignment was carried out using a computer-based urn randomization program. The primary outcome was drug and alcohol use abstinence at 6-month follow up. Analysis was based on data from 116 participants with complete outcome data. Multivariable logistic regression revealed that the DBT-CM program remained an independent positive predictor of decrease in drug use among the DBT-CM participants at 6 months (p = .01) as compared with the HP program participants. Being non-White (p < .05) and having higher depressive symptom scores (p < .05) were associated with lower odds of drug use abstinence (i.e., increased the odds of drug use) at 6 months. DBT-CM increased drug and alcohol abstinence at 6-month follow-up, compared to an HP program.

Association of leptospiral seroreactivity and breed with uveitis and blindness in horses: 372 cases (1986-1993).

PubMed

Dwyer, A E; Crockett, R S; Kalsow, C M

1995-11-15

Recurrent uveitis, a leading cause of blindness in horses, often develops as a sequela to systemic leptospirosis. Over a 7-year period, 63 of 112 (56%) horses with uveitis were seropositive for Leptospira interrogans serovar pomona, but only 23 of 260 (9%) horses without uveitis were seropositive. Odds-ratio analysis revealed that seropositive horses were 13.2 times more likely to have uveitis than were seronegative horses. Of the 63 seropositive horses with uveitis, 59% developed blindness, compared with only 24% in the 49 seronegative horses with uveitis that lost vision in 1 or both eyes during the same period. Odds-ratio analysis revealed that seropositive horses with uveitis were 4.4 times more likely to lose vision than were seronegative horses with uveitis. Of the 112 horses with uveitis, 28 (25%) were Appaloosas, compared with only 10 of the 260 (4%) horses without uveitis (odds ratio, 8.3). In addition, 19 of the 28 (68%) Appaloosas with uveitis developed blindness, compared with only 30 of the 84 (36%) non-Appaloosas with uveitis that lost vision in 1 or both eyes (odds ratio, 3.8). This field study therefore confirmed a strong positive relationship between uveitis and leptospiral seroreactivity in horses. Furthermore, the data suggested that seropositive horses with uveitis were at increased risk of losing vision, compared with that in seronegative horses with uveitis, and that Appaloosas were at increased risk of developing uveitis and associated blindness, compared with that in non-Appaloosas.

Spectroscopy of TaN in Support of Fundamental Physics

NASA Astrophysics Data System (ADS)

Mawhorter, Richard; Sharfi, David; Kim, Yongrak; Kokkin, Damian; Bouchard, Jacob; Steimle, Timothy

2016-05-01

Tantalum nitride, TaN, has been recently identified as a leading candidate for extending the study of T, P-odd effects in the nuclear realm to include proton, neutron, and quark electric dipole moments (EDM) and beyond. This is primarily due to enhancements in the interaction of electrons with the nuclear magnetic quadrupole moment (MQM) and the resulting parity-violating effects. Study of the dispersed laser induced fluorescence resulting from the excitation of the 17570.80 (Ω = 0+) , 18427.38 (Ω = 0+) , 19216.80 (Ω = 1), and 19396.78 (Ω = 1) bands above the X1Σ+ (v = 0) ground state of TaN near 569 nm, 543 nm, 520 nm, and 515 nm has enabled a determination of the branching ratios and transition dipole moments of all 4 states. Radiative lifetimes of 454(32) ns, 479(12) ns, 333(4) ns, and 480(17) ns respectively were measured from an analysis of the fluorescence decay curves, and potential optical pumping approaches for both populating and detecting the parity-violation sensitive 3Δ1 state are proposed. Further experiments using CW laser excitation have enabled the observation of the hyperfine structure of several bands in the gateway 18427.38 (Ω = 0+) to X1Σ+ (v = 0) transition, and analysis of these complex spectra is underway. DK, JB, and TS acknowledge support from NSF CHE-1265885, as do RM, DS, and YK from Pomona College.

Protection of dogs against canine distemper by vaccination with a canarypox virus recombinant expressing canine distemper virus fusion and hemagglutinin glycoproteins.

PubMed

Pardo, M C; Bauman, J E; Mackowiak, M

1997-08-01

To evaluate the safety and efficacy of a live canarypox virus recombinant-canine distemper virus (CDV) combination vaccine against virulent CDV challenge exposure, and to document lack of interference among the other modified-live virus (MLV) components. 33 specific-pathogen-free (SPF) Beagle pups (7 to 10 weeks old). A canarypox virus recombinant-CDV combination vaccine was tested for safety and efficacy along with MLV components (canine adenovirus type 2, canine coronavirus, canine parainfluenza virus, and canine parvovirus) in 26 SPF Beagle pups. The combination vaccine was rehydrated with either Leptospira canicola-L icterohaemorrhagiae combination bacterin (vaccine 1) or sterile diluent (vaccine 2). An additional group of 7 seronegative SPF pups received the control MLV components devoid of the combination vaccine (vaccine 3). Two vaccinations were administered 21 days apart, either IM or SC. The dose of the combination vaccine used to inoculate these pups was 40 times lower than the recommended commercial dose. At 21 days after the booster vaccination, all pups were challenge exposed with a virulent CDV strain, then were observed for 21 days to record morbidity and mortality. Adverse local or generalized reactions were not induced by vaccinations. All vaccinates seroconverted to CDV. Serum antibody titers to MLV components were not different, with or without inclusion of the combination vaccine. After challenge exposure, morbidity and mortality in vaccinates were 0% (0/26); in control dogs, values were 100% morbidity and 86% mortality (6/7). Brain impression smear slides made from all dogs that did not survive challenge exposure were CDV positive by use of a direct fluorescein isothiocyanate method. The canarypox virus-CDV combination vaccine, administered SC or IM, is a safe product that elicits CDV seroconversion, does not interfere with other vaccine components, and protects vaccinated pups against virulent CDV challenge exposure.

Preliminary Investigations on the Distribution of Leptospira Serovars in Domestic Animals in North-west Morocco.

PubMed

Benkirane, A; Noury, S; Hartskeerl, R A; Goris, M G A; Ahmed, A; Nally, J E

2016-04-01

Leptospirosis is a neglected zoonosis of global importance with a complex epidemiology that affects humans, domestic and wild mammals. However, due to the diversity of clinical signs and difficulties of establishing a confirmatory laboratory diagnosis, the disease remains poorly investigated, particularly in the developing world. In Morocco, a descriptive study of the seroprevalence of Leptospira infection in animals has never been undertaken. To fill this gap, the current study was conducted on a subset of animals in north-west Morocco as a preliminary step towards understanding the epidemiological patterns of animal leptospirosis in the country. The study was conducted on 289 serum samples collected between January and April 2012 from dogs, cattle, sheep, goats and donkeys in the areas of Rabat-Temara, Sidi Kacem and Oulmes. All serum samples were tested by the MAT with 14 reference strains of the most prevalent pathogenic serovars of Leptospira and two serovars of non-pathogenic Leptospira. The overall seroprevalence of Leptospira in cattle, sheep, goats, dogs and donkeys was 15%, 18%, 20%, 21% and 20%, respectively. The most prevalent serogroups found in each species were Ballum, Sejroe, and Australis in cattle, Ballum, Australis and Sejroe in sheep, Australis and Ballum in goats, Javanica and Australis in donkey and Australis, Ballum and Canicola in dogs. Of all the serogroups tested in this study, Icterohaemorrhagiae, the only serogroup which has been previously reported in humans in Morocco, was rarely reactive. The majority of reactive sera were collected from low land areas. A large number of sera samples classified as seronegative when tested against pathogenic leptospires were positive when tested against non-pathogenic leptospires; this is suggestive of possible novel, as yet unclassified, Leptospira serovars in Morocco. Eleven of thirteen sheep urine samples were positive by real-time PCR confirming their role as Leptospira carriers in Morocco. © 2014

[The use of chest X-rays for surveillance of bacterial pneumonias in children in Latin America].

PubMed

Lagos, Rosanna; di Fabio, José Luis; Moënne, Karla; Muñoz M, Alma; Wasserman, Steven; de Quadros, Ciro

2003-05-01

The Division of Vaccines and Immunization of the Pan American Health Organization (PAHO) is promoting epidemiological surveillance of bacterial pneumonias in children in Latin America in order to generate scientific evidence to support future decisions concerning using vaccines to control such pneumonias in the countries of the Region of the Americas. The diagnosis of these diseases rarely includes bacteriological documentation of the causative agent. Therefore, studies of this type that are carried out around the world accept radiological images of alveolar consolidation as a confirmatory criterion for a presumptively bacterial pneumonia. This piece examines the theoretical rationale and requirements for using thorax radiology as an instrument for epidemiological surveillance of bacterial pneumonias. The piece also summarizes the activities carried out during 2 years of joint efforts between the Center for Vaccine Development (Centro para Vacunas en Desarrollo) of Chile and PAHO's Division of Vaccines and Immunization. During those 2 years, the two groups encouraged the epidemiological study of bacterial pneumonias in Latin American children, using internationally accepted criteria and definitions as well as tools and practical solutions adapted to the reality of the Region of the America. The activities carried out so far show both the need for and the feasibility of standardizing the interpretation of chest radiographs so that they can be used in epidemiological studies.

Investigating risk factors and possible infectious aetiologies of mummified fetuses on a large piggery in Australia.

PubMed

Dron, N; Hernández-Jover, M; Doyle, R E; Holyoake, P K

2014-12-01

To investigate risk factors and potential infectious aetiologies of an increased mummification rate (>2%) identified over time on a 1200-sow farrow-to-finish farm in Australia. Association of potential non-infectious risk factors and the mummification rate was investigated using 15 years of breeding herd data (40,940 litters) and logistic regression analysis. Samples from a limited number of mummified fetuses were taken to identify potential infectious aetiologies (porcine parvovirus, Leptospira pomona, porcine circovirus type 2, Bungowannah virus and enterovirus). Logistic regression analysis suggested that the mummification rate was significantly associated with sow breed and parity, year and total born and stillborn piglets per litter. The mummification rate was lower (P < 0.001) in Landrace (3.4%) and Large White (2.6%) sows than in Duroc sows (4.9%). Gilts (2.9%) had a lower (P < 0.001) mummification rate than older sows. The mummification rate increased with total born litter size and decreased with the number of stillborn piglets (P < 0.001). A clustering effect within individual sows was identified, indicating that some sows with mummified fetuses in a litter were more likely to have repeated mummifications in subsequent litters. No infectious agents were identified in the samples taken. Results from this study suggest that the increased mummification rate identified over time on this farm is likely to be a non-infectious multifactorial problem predisposing the occurrence of mummification. Further research is required to better understand the pathophysiology of mummification and the role that different non-infectious factors play in the occurrence of mummified fetuses. © 2014 Australian Veterinary Association.

Serologic Survey for Selected Viral and Bacterial Swine Pathogens in Colombian Collared Peccaries ( Pecari tajacu) and Feral Pigs ( Sus scrofa).

PubMed

Montenegro, Olga L; Roncancio, Nestor; Soler-Tovar, Diego; Cortés-Duque, Jimena; Contreras-Herrera, Jorge; Sabogal, Sandra; Acevedo, Luz Dary; Navas-Suárez, Pedro Enrique

2018-06-14

In South America, wild populations of peccaries coexist with domestic and feral pigs, with poorly understood consequences. We captured 58 collared peccaries ( Pecari tajacu) and 15 feral pigs ( Sus scrofa) in locations of Colombia where coexistence of these species is known. Blood samples were tested for antibodies against four viral agents, classical swine fever virus (CSFV), Aujeszky's disease virus (ADV), porcine circovirus (PCV-2), and vesicular stomatitis virus (New Jersey and Indiana subtypes) and two bacterial agents, Brucella spp. and six serovars of Leptospira interrogans. The prevalence of CSFV was 5% (3/58) in collared peccaries and 7% (1/15) in feral pigs. The prevalence of PCV-2 was 7% (1/15) in collared peccaries and 67% (2/3) in feral pigs. Vesicular stomatitis prevalence was 33% (8/24) in collared peccaries and 67% (4/6) in feral pigs. Leptospira prevalence was 78% (39/50) in collared peccary and 100% (8/8) in feral pigs; bratislava, grippotyphosa, icterohaemorrhagiae, and pomona were the most frequent serovars. Also, the only white-lipped peccary ( Tayassu pecari) sampled was positive for L. interrogans serovar bratislava and for vesicular stomatitis virus, New Jersey strain. No samples were positive for ADV or Brucella. The seroprevalence of antibodies against L. interrogans was similar to that observed in other studies. Icterohaemorrhagiae appears to be a common serovar among in situ and ex situ peccary populations. Positive antibodies against PVC-2 represent a novel report of exposure to this pathogen in Colombian peccaries. Our results indicate the possible transmission of various pathogens, important for pig farms, in the studied pig and peccaries.

Cloning and Molecular Characterization of an Immunogenic LigA Protein of Leptospira interrogans

PubMed Central

Palaniappan, Raghavan U. M.; Chang, Yung-Fu; Jusuf, S. S. D.; Artiushin, S.; Timoney, John F.; McDonough, Sean P.; Barr, Steve C.; Divers, Thomas J.; Simpson, Kenneth W.; McDonough, Patrick L.; Mohammed, Hussni O.

2002-01-01

A clone expressing a novel immunoreactive leptospiral immunoglobulin-like protein A of 130 kDa (LigA) from Leptospira interrogans serovar pomona type kennewicki was isolated by screening a genomic DNA library with serum from a mare that had recently aborted due to leptospiral infection. LigA is encoded by an open reading frame of 3,675 bp, and the deduced amino acid sequence consists of a series of 90-amino-acid tandem repeats. A search of the NCBI database found that homology of the LigA repeat region was limited to an immunoglobulin-like domain of the bacterial intimin binding protein of Escherichia coli, the cell adhesion domain of Clostridium acetobutylicum, and the invasin of Yersinia pestis. Secondary structure prediction analysis indicates that LigA consists mostly of beta sheets with a few alpha-helical regions. No LigA was detectable by immunoblot analysis of lysates of the leptospires grown in vitro at 30°C or when cultures were shifted to 37°C. Strikingly, immunohistochemistry on kidney from leptospira-infected hamsters demonstrated LigA expression. These findings suggest that LigA is specifically induced only in vivo. Sera from horses, which aborted as a result of natural Leptospira infection, strongly recognize LigA. LigA is the first leptospiral protein described to have 12 tandem repeats and is also the first to be expressed only during infection. Thus, LigA may have value in serodiagnosis or as a protective immunogen in novel vaccines. PMID:12379666

Lanthanide oxide and phosphate nanoparticles for thermometry and bimodal imaging =

NASA Astrophysics Data System (ADS)

Debasu, Mengistie Leweyehu

Nesta tese relatam-se estudos de fotoluminescencia de nanoparticulas de oxidos e fosfatos dopados com ioes trivalentes de lantanideos, respectivamente, nanobastonetes de (Gd,Eu)2O3 e (Gd,Yb,Er)2O3 e nanocristais de (Gd,Yb,Tb)PO4, demonstrando-se tambem aplicacoes destes materiais em revestimentos inteligentes, sensores de temperatura e bioimagem. Estuda-se a transferencia de energia entre os sitios de Eu3+ C2 e S6 dos nanobastonetes Gd2O3. A contribuicao dos mecanismos de transferencia de energia entre sitios para o tempo de subida 5D0(C2) e descartada a favor da relaxacao directa 5D1(C2) 5D0(C2) (i.e., transferencia de energia entre niveis). O maior tempo de decaimento do nivel 5D0(C2) nos nanobastonetes, relativamente ao valor medido para o mesmo material na forma de microcristais, e atribuido, quer a existencia de espacos livres entre nanobastonetes proximos (factor de enchimento ou fraccao volumica), quer a variacao do indice de refraccao efectivo do meio em torno dos ioes Eu3+. A dispersao de nanobastonetes de (Gd,Eu)2O3 em tres resinas epoxi comerciais atraves da cura por UV permite obter nanocompositos epoxi- (Gd,Eu)2O3. Relatam-se estudos cineticos e das propriedades termicas e de fotoluminescencia destes nanocompositos. Estes, preservam as tipicas propriedades de emissao do Eu3+, mostrando o potencial do metodo de cura por UV para obter revistimentos inteligentes e fotoactivos. Considera-se um avanco significativo a realizacao de uma nanoplataforma optica, incorporando aquecedor e termometro e capaz de medir uma ampla gama de temperaturas (300-2000 K) a escala nano, baseada em nanobastonetes de (Gd,Yb,Er)2O3 (termometros) cuja superficie se encontra revestida com nanoparticulas de ouro. A temperature local e calculada usando, quer a distribuicao de Boltzmann (300-1050 K) do racio de intensidades da conversao ascendente 2H11=2!4I15=2/4S3=2!4I15=2, quer a lei de Planck (1200-2000 K) para uma emissao de luz branca atribuida a radiacao do corpo negro

[Health information on the Internet and trust marks as quality indicators: vaccines case study].

PubMed

Mayer, Miguel Angel; Leis, Angela; Sanz, Ferran

2009-10-01

To find out the prevalence of quality trust marks present in websites and to analyse the quality of these websites displaying trust marks compared with those that do not display them, in order to put forward these trust marks as a quality indicator. Cross-sectional study. Internet. Websites on vaccines. Using "vacunas OR vaccines" as key words, the features of 40 web pages were analysed. These web pages were selected from the page results of two search engines, Google and Yahoo! Based on a total of 9 criteria, the average score of criteria fulfilled was 7 (95% CI 3.96-10.04) points for the web pages offered by Yahoo! and 7.3 (95% CI 3.86-10.74) offered by Google. Amongst web pages offered by Yahoo!, there were three with clearly inaccurate information, while there were four in the pages offered by Google. Trust marks were displayed in 20% and 30% medical web pages, respectively, and their presence reached statistical significance (P=0.033) when fulfilling the quality criteria compared with web pages where trust marks were not displayed. A wide variety of web pages was obtained by search engines and a large number of them with useless information. Although the websites analysed had a good quality, between 15% and 20% showed inaccurate information. Websites where trust marks were displayed had more quality than those that did not display one and none of them were included amongst those where inaccurate information was found.

Distribution of cytotoxic and DNA ADP-ribosylating activity in crude extracts from butterflies among the family Pieridae

PubMed Central

Matsumoto, Yasuko; Nakano, Tsuyoshi; Yamamoto, Masafumi; Matsushima-Hibiya, Yuko; Odagiri, Ken-Ichi; Yata, Osamu; Koyama, Kotaro; Sugimura, Takashi; Wakabayashi, Keiji

2008-01-01

Cabbage butterflies, Pieris rapae and Pieris brassicae, contain strong cytotoxic proteins, designated as pierisin-1 and -2, against cancer cell lines. These proteins exhibit DNA ADP-ribosylating activity. To determine the distribution of substances with cytotoxicity and DNA ADP-ribosylating activity among other species, crude extracts from 20 species of the family Pieridae were examined for cytotoxicity in HeLa cells and DNA ADP-ribosylating activity. Both activities were detected in extracts from 13 species: subtribes Pierina (Pieris rapae, Pieris canidia, Pieris napi, Pieris melete, Pieris brassicae, Pontia daplidice, and Talbotia naganum), Aporiina (Aporia gigantea, Aporia crataegi, Aporia hippia, and Delias pasithoe), and Appiadina (Appias nero and Appias paulina). All of these extracts contained substances recognized by anti-pierisin-1 antibodies, with a molecular mass of ≈100 kDa established earlier for pierisin-1. Moreover, sequences containing NAD-binding sites, conserved in ADP-ribosyltransferases, were amplified from genomic DNA from 13 species of butterflies with cytotoxicity and DNA ADP-ribosylating activity by PCR. Extracts from seven species, Appias lyncida, Leptosia nina, Anthocharis scolymus, Eurema hecabe, Catopsilia pomona, Catopsilia scylla, and Colias erate, showed neither cytotoxicity nor DNA ADP-ribosylating activity, and did not contain substances recognized by anti-pierisin-1 antibodies. Sequences containing NAD-binding sites were not amplified from genomic DNA from these seven species. Thus, pierisin-like proteins, showing cytotoxicity and DNA ADP-ribosylating activity, are suggested to be present in the extracts from butterflies not only among the subtribe Pierina, but also among the subtribes Aporiina and Appiadina. These findings offer insight to understanding the nature of DNA ADP-ribosylating activity in the butterfly. PMID:18256183

International Field Research with Undergraduate Students: Investigating Active Tectonics of the Nicoya Peninsula, Costa Rica

NASA Astrophysics Data System (ADS)

Marshall, J. S.; Gardner, T. W.; Protti, M.

2005-12-01

Over the past eight years, 18 undergraduate students from 12 U.S. and Costa Rican universities and colleges have participated in field research projects investigating coastal tectonics on the Nicoya Peninsula, Costa Rica. These projects have been organized around two different models: 1) a month-long "field camp" with 10 students and 5 project faculty (Keck Geology Consortium Project, 1998), and 2) several two-week field projects with 1-3 students and one faculty advisor (Cal Poly Pomona University and Trinity University). Under the direction of the authors, each of these projects has been carefully designed to provide a new piece to a larger research puzzle. The Nicoya Peninsula lies along Costa Rica's northern Pacific coast inboard of the Middle America Trench where the Cocos and Caribbean plates converge at 10 cm/yr. In 1950, the peninsula was shaken by a ~M 7.7 subduction earthquake that produced widespread damage and 0.5-1.0 m of coseismic coastal uplift. With a large slip deficit since 1950, the Nicoya Peninsula is viewed as a high-potential seismic gap. Field study of uplifted Quaternary marine terraces along the Nicoya coastline provides undergraduate students with a unique opportunity to examine rapid forearc deformation related to large subduction earthquakes. The field research conducted by each of these students provides the basis for a senior thesis at their home institution. In most cases, the students have focused their individual work on separate, but adjacent field areas. Collectively, each of these projects has generated significant data that contribute toward of an ongoing investigation of fore arc tectonics and subduction cycle earthquakes along the Costa Rican Pacific margin.

Lsa63, a newly identified surface protein of Leptospira interrogans binds laminin and collagen IV.

PubMed

Vieira, Monica L; de Morais, Zenaide M; Gonçales, Amane P; Romero, Eliete C; Vasconcellos, Silvio A; Nascimento, Ana L T O

2010-01-01

Leptospira interrogans is the etiological agent of leptospirosis, a zoonotic disease that affects populations worldwide. We have identified in proteomic studies a protein that is encoded by the gene LIC10314 and expressed in virulent strain of L. interrogans serovar Pomona. This protein was predicted to be surface exposed by PSORT program and contains a p83/100 domain identified by BLAST analysis that is conserved in protein antigens of several strains of Borrelia and Treponema spp. The proteins containing this domain have been claimed antigen candidates for serodiagnosis of Lyme borreliosis. Thus, we have cloned the LIC10314 and expressed the protein in Escherichia coli BL21-SI strain by using the expression vector pAE. The recombinant protein tagged with N-terminal hexahistidine was purified by metal-charged chromatography and characterized by circular dichroism spectroscopy. This protein is conserved among several species of pathogenic Leptospira and absent in the saprophytic strain L. biflexa. We confirm by liquid-phase immunofluorescence assays with living organisms that this protein is most likely a new surface leptospiral protein. The ability of the protein to mediate attachment to ECM components was evaluated by binding assays. The leptospiral protein encoded by LIC10314, named Lsa63 (Leptospiral surface adhesin of 63kDa), binds strongly to laminin and collagen IV in a dose-dependent and saturable fashion. In addition, Lsa63 is probably expressed during infection since it was recognized by antibodies of serum samples of confirmed-leptospirosis patients in convalescent phase of the disease. Altogether, the data suggests that this novel identified surface protein may be involved in leptospiral pathogenesis. 2009 The British Infection Society. Published by Elsevier Ltd. All rights reserved.

Plurality of Leptospira strains on slaughtered animals suggest a broader concept of adaptability of leptospires to cattle.

PubMed

Pinto, Priscila S; Pestana, Cristiane; Medeiros, Marco A; Lilenbaum, Walter

2017-08-01

Leptospirosis in bovines is in majority determined by the host-adapted serovars, mainly Hardjo (types Hardjoprajitno and Hardjobovis), that belong to the serogroup Sejroe. Members of other serogroups as Pomona and Tarassovi have been eventually reported, mainly when outbreaks occurs. Nevertheless, the real role of other strains (non-Hardjo) on determining disease or being transmitted by cattle free of apparent clinical signs of acute infection remains to be elucidated. In that context, the aim of the present study was to investigate the hypothesis that strains of serovars/serogroups other than Hardjo may also be maintained and shed by cattle free of clinical signs. Samples of urine and/or vaginal fluid were collected from 697 bovines from a slaughterhouse located close to Rio de Janeiro, Brazil. Culturing yielded 19 isolates what represents the largest number ever obtained in Brazil on similar studies. These strains were serogrouped and genetically characterized. Fifteen of those were described in other papers and four are first described on the present study. Isolates belong to three different species (Leptospira santarosai, L. alstonii and L. interrogans) and five serogroups (Sarmin, Tarassovi, Shermani, Grippotyphosa and Sejroe). The majority (84.2%) of the isolates belongs to the species L. santarosai, the most prevalent species on cattle in the studied region. Non-Hardjo (non-Sejroe) strains represent 57.9% of the isolates, what indicates an unexpected high diversity of serogroups obtained from these cattle. This suggest that non-Hardjo (non-Sejroe) strains may also be maintained and shed by cattle and that finding must be considered in the epidemiology and control of the disease. Copyright © 2017 Elsevier B.V. All rights reserved.

Outbreaks of Salmonellosis From Small Turtles.

PubMed

Walters, Maroya Spalding; Simmons, Latoya; Anderson, Tara C; DeMent, Jamie; Van Zile, Kathleen; Matthias, Laura P; Etheridge, Sonia; Baker, Ronald; Healan, Cheryl; Bagby, Rita; Reporter, Roshan; Kimura, Akiko; Harrison, Cassandra; Ajileye, Kadri; Borders, Julie; Crocker, Kia; Smee, Aaron; Adams-Cameron, Meg; Joseph, Lavin A; Tolar, Beth; Trees, Eija; Sabol, Ashley; Garrett, Nancy; Bopp, Cheryl; Bosch, Stacey; Behravesh, Casey Barton

2016-01-01

Turtle-associated salmonellosis (TAS), especially in children, is a reemerging public health issue. In 1975, small pet turtles (shell length <4 inches) sales were banned by federal law; reductions in pediatric TAS followed. Since 2006, the number of multistate TAS outbreaks has increased. We describe 8 multistate outbreaks with illness-onset dates occurring in 2011-2013. We conducted epidemiologic, environmental, and traceback investigations. Cases were defined as infection with ≥ 1 of 10 molecular subtypes of Salmonella Sandiego, Pomona, Poona, Typhimurium, and I 4,[5],12:i:-. Water samples from turtle habitats linked to human illnesses were cultured for Salmonella. We identified 8 outbreaks totaling 473 cases from 41 states, Washington DC, and Puerto Rico with illness onsets during May 2011-September 2013. The median patient age was 4 years (range: 1 month-94 years); 45% percent were Hispanic; and 28% were hospitalized. In the week preceding illness, 68% (187 of 273) of case-patients reported turtle exposure; among these, 88% (124 of 141) described small turtles. Outbreak strains were isolated from turtle habitats linked to human illnesses in seven outbreaks. Traceback investigations identified 2 Louisiana turtle farms as the source of small turtles linked to 1 outbreak; 1 outbreak strain was isolated from turtle pond water from 1 turtle farm. Eight multistate outbreaks associated with small turtles were investigated during 2011-2013. Children <5 years and Hispanics were disproportionately affected. Prevention efforts should focus on patient education targeting families with young children and Hispanics and enactment of state and local regulations to complement federal sales restrictions. Copyright © 2016 by the American Academy of Pediatrics.

An online review of plastic surgeons in southern California.

PubMed

Lewis, Priya; Kobayashi, Emily; Gupta, Subhas

2015-05-01

It has become commonplace for patients to access online reviews of physicians when making choices about health care, just as any consumer would in today's computer-dependent world. Previous studies have shown that online reviews of physicians are generally positive. However, 1 negative review has the potential to adversely affect business and reputations. To characterize the online presence of plastic surgeons in Southern California as portrayed by physician rating websites (PRWs). An extensive online database of board-certified plastic surgeons was used to generate a list of surgeons within a 50-mile radius of Pomona, CA. Ratings from the PRWs HealthGrades.com, Vitals.com, and UCompareHealthcare.com were cataloged by number of reviews and ratings. Two hundred sixty-three surgeons were evaluated with the most-represented cities being Beverly Hills (N=47), Los Angeles (N=31), and Newport Beach (N=27). Ninety-seven percent of the surgeons were rated on at least 1 of the 3 PRWs chosen. In general, surgeons were rated highly, with a mean rating of 85%, SD, 14% (P<0.01), with a mean of 11.0 ratings per PRW, SD 10.9 (P<0.01). Total online ratings ranged from 0 to 222 per surgeon. The median number of total reviews was 25 and the mean rating for those surgeons above and below the median were equivocal, at 86% and 85%, respectively (P=0.284). In this study, we found that plastic surgeons in Southern California have an online presence that can be influenced by their patients; they should be aware of this and conscious of their online reputations. Overall, the ratings were high, regardless of the number of reviews.

Survey for selected pathogens in wild pigs (Sus scrofa) from Guam, Marianna Islands, USA.

PubMed

Cleveland, Christopher A; DeNicola, Anthony; Dubey, J P; Hill, Dolores E; Berghaus, Roy D; Yabsley, Michael J

2017-06-01

Pigs (Sus scrofa) were introduced to Guam in the 1600's and are now present in high densities throughout the island. Wild pigs are reservoirs for pathogens of concern to domestic animals and humans. Exposure to porcine parvovirus, transmissible gastroenteritis, and Leptospira interrogans has been documented in domestic swine but data from wild pigs are lacking. The close proximity of humans, domestic animals, and wild pigs, combined with the liberal hunting of wild pigs, results in frequent opportunities for pathogen transmission. From February-March 2015, blood, tissue and ectoparasite samples were collected from 47 wild pigs. Serologic testing found exposure to Brucella spp. (2%), Toxoplasma gondii (11%), porcine reproductive and respiratory syndrome (PRRS) virus (13%), porcine circovirus type 2 (36%), pseudorabies virus (64%), Actinobacillus pleuropneumoniae (93%), Lawsonia intracellularis (93%), and porcine parvovirus (94%). Eleven (24%) samples had low titers (1:100) to Leptospira interrogans serovars Bratislava (n=6), Icterohaemorrhagiae (n=6), Pomona (n=2), and Hardjo (n=1). Kidney samples from nine pigs with Leptospira antibodies were negative for Leptospira antigens. Numerous pigs had Metastrongylus lungworms and three had Stephanurus dentatus. Lice (Hematopinus suis) and ticks (Amblyomma breviscutatum) were also detected. No antibodies to Influenza A viruses were detected. In contrast to the previous domestic swine survey, we found evidence of numerous pathogens in wild pigs including new reports of pseudorabies virus, PRRS virus, Brucella, and Leptospira in pigs on Guam. These findings highlight that domestic swine-wild pig interactions should be prevented and precautions are needed when handling wild pigs to minimize the risk of pathogen transmission. Copyright © 2017 Elsevier B.V. All rights reserved.

Inquiry-Based Learning in an Intermediate-Level Undergraduate Neotectonics Course

NASA Astrophysics Data System (ADS)

Reinen, L. A.

2007-12-01

Integrating student-conducted research into the curriculum can provide students with many educational benefits. Documented benefits include, among others, increased communication skills, the ability to work as part of a research team, and enhanced self-confidence in individual problem-solving skills (e.g., Kardash, J. Ed. Psych., 2000; Seymour, et al., Science Education, 2004). As part of a larger departmental goal of integrating student- conducted research into all levels of the Pomona College Geology Department curriculum (e.g., Reinen, et al., CUR-Q, 2006), I have recently developed an intermediate-level Neotectonics course with a strong component of inquiry-based learning. This course was offered for the first time during the spring semester 2007, and will continue to be offered each year. In a series of guided inquiries throughout the course, students investigate recent seismicity and tectonic geomorphology in Southern California. With each subsequent assignment, student contributions to the research direction increases (e.g., data used, area studied, question addressed, methods used), culminating in team proposals and research projects investigating specific student-generated questions of regional tectonics. Students collect data for these investigations from several sources: (1) databases available online (e.g., IRIS, Harvard earthquake catalog), (2) desktop experiments (e.g., the "earthquake machine"), (3) topographic maps, and (4) field observations. The objective of this paper is to present initial results from this teaching experiment and examples of the projects which have been executed, including the preparation students received to be able to use the available data. Discussion and suggestions (particularly about effective means of conducting a rigorous long-term assessment) are strongly encouraged.

Distribution of capsular types and antimicrobial susceptibility of invasive isolates of Streptococcus pneumoniae in Colombian children. Pneumococcal Study Group in Colombia.

PubMed

Castañeda, E; Leal, A L; Castillo, O; De La Hoz, F; Vela, M C; Arango, M; Trujillo, H; Levy, A; Gama, M E; Calle, M; Valencia, M L; Parra, W; Agudelo, N; Mejía, G I; Jaramillo, S; Montoya, F; Porras, H; Sánchez, A; Saa, D; Di Fabio, J L; Homma, A

1997-01-01

Streptococcus pneumoniae is the leading bacterial cause of childhood pneumonia in the developing world. This study describes the type distribution and antimicrobial susceptibility of invasive pneumococcal isolates from Colombian children and is part of the Sistema Regional de Vacunas (SIREVA), a PAHO regional initiative designed to determine the ideal serotype composition of a protein polysaccharide pneumococcal conjugate vaccine for use in children less than 5 years old in Latin America. In Colombia, during the study period, centres in Bogota, Medellin, and Cali collected 324 S. pneumoniae isolates from invasive diseases, 238 (73.5%) from children under the age of 2. Pneumonia was the clinical diagnosis in 41.3% cases, meningitis in 41%, and sepsis in 11.2%. The seven most frequent types included 14(21.9%), 5(10.5%), 23F(9.6%), 1(9%), 6B(9%), 19F(7.1%), and 6A(6.2%). The frequency of diminished susceptibility to penicillin (DSP) was 12%, with 8.9% of isolates showing intermediate level resistance and 3.1% showing high level resistance. Among DSP isolates, 23% were also resistant to cefotaxime, 33.3% to erythromycin, 48.7% to chloramphenicol, and 74.3% to trimethoprim/sulfamethoxazole. Multiple resistance was detected in 59% of the isolates that have DSP. Penicillin resistance was associated with types 23F (53.8%) and 14 (25.6%). These data provides information on capsular types prevalent in Colombia that will not only allow the formulation of an ideal vaccine for the region but also reinforce the need for ongoing regional surveillance.

Student performance on levels 1 and 2-CE of COMLEX-USA: do elective upper-level undergraduate science courses matter?

PubMed

Wong, Stanley K; Ramirez, Juan R; Helf, Scott C

2009-11-01

The effect of a variety of preadmission variables, including the number of elective preadmission upper-level science courses, on academic achievement is not well established. To investigate the relationship between number of preadmission variables and overall student academic achievement in osteopathic medical school. Academic records of osteopathic medical students in the 2008 and 2009 graduating classes of Western University of Health Sciences College of Osteopathic Medicine of the Pacific in Pomona, California, were analyzed. Multivariate linear regression analyses were performed to identify predictors of academic achievement based on Medical College Admission Test (MCAT) subscores, undergraduate grade point average (GPA), GPA in medical school basic science (preclinical GPA) and clinical clerkship (clinical GPA), and scores on the Comprehensive Osteopathic Medical Licensing Examination-USA (COMLEX-USA) Level 1 and Level 2-Cognitive Evaluation (CE). Records of 358 osteopathic medical students were evaluated. Analysis of beta coefficients suggested that undergraduate science GPA was the most important predictor of overall student academic achievement (P<.01). Biological sciences MCAT subscore was a more modest but still statistically significant predictor of preclinical GPA and COMLEX-USA Level 1 score (P<.01). Physical sciences MCAT subscore was also a statistically significant predictor of preclinical GPA, and verbal reasoning MCAT subscore was a statistically significant predictor of COMLEX-USA Level 2-CE score (both P<.01). Women had statistically significantly higher preclinical GPA and COMLEX-USA Level 2-CE scores than men (P<.05). Differences in some outcome variables were also associated with racial-ethnic background and age. Number of preadmission elective upper-level science courses taken by students before matriculation was not significantly correlated with any academic achievement variable. Although undergraduate science GPA and MCAT biological

Resurgence of leptospirosis in dogs in Ontario: recent findings

PubMed Central

Prescott, John F.; McEwen, Beverly; Taylor, Judith; Woods, J. Paul; Abrams-Ogg, Anthony; Wilcock, Brian

2002-01-01

A marked increase in leptospirosis in dogs was observed in 2000, part of an increasing trend observed in previous years in Ontario. The highest frequency of seropositive cases occurred from September to December 2000, with the peak in November. Large breed dogs were particularly affected. Clinical and clinicopathological data for 31 dogs admitted between 1998 and 2000 to the Ontario Veterinary College Veterinary Teaching Hospital were analyzed. Major clinical presenting features were acute onset of anorexia, depression, fever, and vomiting. Ninety percent of dogs, on admission, showed biochemical evidence of injury to several organs, notably combinations in the order of kidney, muscle, pancreas, and liver. Almost all dogs showed increased serum urea and creatinine levels, and the majority had increased total creatine kinase, bilirubin, alkaline phosphatase, and leukocytosis with neutrophilia. One-third were thrombocytopenic. Of dogs with liver-related abnormalities, most had evidence of cholestasis, with or without hepatocellular damage. Based on serologic studies, in the year 2000, the major serovar involved was autumnalis, but bratislava, grippotyphosa, and pomona were also implicated. The microscopic agglutination test often gave a confusing pattern of reactivities to the serovars that were tested. The high reactivity to serovar autumnalis may represent an erroneous or “paradoxical” reaction typical of early leptospiral serology. The year 2000 was the warmest in Ontario in each of the 4 fall months (September–December) of the previous decade, as well as being the third wettest in the fall period in the last decade. The increase in canine leptospirosis, therefore, may, in part, reflect climate change. The number of positive cases declined in 2001 by about one-third of those in 2000, but the number of submissions of sera for diagnosis increased markedly over previous years. Further work is required to isolate and to identify definitively serovars involved in

How Important Are Rats As Vectors of Leptospirosis in the Mekong Delta of Vietnam?

PubMed Central

Loan, Hoang Kim; Van Cuong, Nguyen; Takhampunya, Ratree; Kiet, Bach Tuan; Campbell, James; Them, Lac Ngoc; Bryant, Juliet E.; Tippayachai, Bousaraporn; Van Hoang, Nguyen; Morand, Serge; Hien, Vo Be

2015-01-01

Abstract Leptospirosis is a zoonosis known to be endemic in the Mekong Delta of Vietnam, even though clinical reports are uncommon. We investigated leptospira infection in rats purchased in food markets during the rainy season (October) (n=150), as well as those trapped during the dry season (February–March) (n=125) in the region using RT-PCR for the lipL32 gene, confirmed by 16S rRNA, as well as by the microscopic agglutination test (MAT). Results were compared with the serovar distribution of human cases referred from Ho Chi Minh City hospitals (2004–2012) confirmed by MAT (n=45). The MAT seroprevalence among rats was 18.3%. The highest MAT seroprevalence corresponded, in decreasing order, to: Rattus norvegicus (33.0%), Bandicota indica (26.5%), Rattus tanezumi (24.6%), Rattus exulans (14.3%), and Rattus argentiventer (7.1%). The most prevalent serovars were, in descending order: Javanica (4.6% rats), Lousiana (4.2%), Copenageni (4.2%), Cynopterie (3.7%), Pomona (2.9%), and Icterohaemorrhagiae (2.5%). A total of 16 rats (5.8%) tested positive by RT-PCR. Overall, larger rats tended to have a higher prevalence of detection. There was considerable agreement between MAT and PCR (kappa=0.28 [0.07–0.49]), although significantly more rats were positive by MAT (McNemar 29.9 (p<0.001). MAT prevalence was higher among rats during the rainy season compared with rats in the dry season. There are no current available data on leptospira serovars in humans in the Mekong Delta, although existing studies suggest limited overlapping between human and rat serovars. Further studies should take into account a wider range of potential reservoirs (i.e., dogs, pigs) as well as perform geographically linked co-sampling of humans and animals to establish the main sources of leptospirosis in the region. PMID:25629781

How important are rats as vectors of leptospirosis in the Mekong Delta of Vietnam?

PubMed

Loan, Hoang Kim; Van Cuong, Nguyen; Takhampunya, Ratree; Kiet, Bach Tuan; Campbell, James; Them, Lac Ngoc; Bryant, Juliet E; Tippayachai, Bousaraporn; Van Hoang, Nguyen; Morand, Serge; Hien, Vo Be; Carrique-Mas, Juan J

2015-01-01

Leptospirosis is a zoonosis known to be endemic in the Mekong Delta of Vietnam, even though clinical reports are uncommon. We investigated leptospira infection in rats purchased in food markets during the rainy season (October) (n=150), as well as those trapped during the dry season (February-March) (n=125) in the region using RT-PCR for the lipL32 gene, confirmed by 16S rRNA, as well as by the microscopic agglutination test (MAT). Results were compared with the serovar distribution of human cases referred from Ho Chi Minh City hospitals (2004-2012) confirmed by MAT (n=45). The MAT seroprevalence among rats was 18.3%. The highest MAT seroprevalence corresponded, in decreasing order, to: Rattus norvegicus (33.0%), Bandicota indica (26.5%), Rattus tanezumi (24.6%), Rattus exulans (14.3%), and Rattus argentiventer (7.1%). The most prevalent serovars were, in descending order: Javanica (4.6% rats), Lousiana (4.2%), Copenageni (4.2%), Cynopterie (3.7%), Pomona (2.9%), and Icterohaemorrhagiae (2.5%). A total of 16 rats (5.8%) tested positive by RT-PCR. Overall, larger rats tended to have a higher prevalence of detection. There was considerable agreement between MAT and PCR (kappa=0.28 [0.07-0.49]), although significantly more rats were positive by MAT (McNemar 29.9 (p<0.001). MAT prevalence was higher among rats during the rainy season compared with rats in the dry season. There are no current available data on leptospira serovars in humans in the Mekong Delta, although existing studies suggest limited overlapping between human and rat serovars. Further studies should take into account a wider range of potential reservoirs (i.e., dogs, pigs) as well as perform geographically linked co-sampling of humans and animals to establish the main sources of leptospirosis in the region.

[Detection of leptospira by culture of vitreous humor and detection of antibodies against leptospira in vitreous humor and serum of 225 horses with equine recurrent uveitis].

PubMed

Dorrego-Keiter, Elisa; Tóth, József; Dikker, Lieke; Sielhorst, Jutta; Schusser, Gerald Fritz

2016-01-01

In the ongoing discussion regarding the aetiopathogenesis of equine recurrent uveitis (ERU) it was the aim of the present study to elucidate the relationship of leptospira infection and ERU. In a population of 225 horses leptospira were examined in vitreous humor by culture and leptospira antibody were detected in vitreous humor and serum samples. Preoperative serum samples were collected from 221/225 ERU patients of different age, gender and breed. Undiluted vitreous humor was aseptically taken from 198/225 patients that underwent pars plana vitrectomy at the beginning of surgery and from 27/225 patients' eyeball after enucleation: Serum and vitreous humor were tested for specific leptospiral antibodies by microscopic agglutination test (MAT). Furthermore, vitreous humor was examined by culture. 20 patients which were euthanized due to a live-threatening disease other than ERU served as a control group. A total of 127/221 (57.5%) horses had serum antibodies (≥ 1:100). Most frequently antibodies against L. interrogans serovar Grippotyphosa were detected (79/127), followed by L. interrogans serovar lcterohaemorrhagiae (34/127) and L. interrogans serovar Bratislava (29/127). Only 79/225 horses (35.1%) had leptospiral antibodies in vitreous humor, in which L. interrogans serovar Grippotyphosa (67/79) was identified most frequently followed by L. interrogans serovar Pomona (18/79) and L. interrogans serovar lcterohaemorrhagiae (8/79) which was identified as single or multiple reaction. Isolation of leptospira from vitreous humor was positive in 34/212 horses (16%). 10/20 control horses had a positive antibody titer against leptospira in serum and 2/20 horses in vitreous humor, whereas there was no leptospira detected in culture. The result of 84% negative cultures from vitreous humor of 212 ERU patients is decisive for the diagnosis and therapy of ERU.

An assessment of zoonotic and production limiting pathogens in rusa deer (Cervus timorensis rusa) from Mauritius.

PubMed

Jori, F; Godfroid, J; Michel, A L; Potts, A D; Jaumally, M R; Sauzier, J; Roger, M

2014-08-01

A population of approximately 70,000 rusa deer (Cervus timorensis russa) represents the most important mammal species reared for food on the island of Mauritius, being the main source of red meat for the local population. However, very limited information is available on the circulation of pathogens affecting the productivity and health of this species. To produce baseline data on the circulation of infectious pathogens in rusa deer under production, a serological survey and/or direct pathogen detection for six selected infectious diseases was undertaken in 2007 in a sample of 53% of the herds reared in semi-free-ranging conditions in hunting estates. Seropositive results were recorded for Johne's disease with an indirect ELISA test (1.7%, n = 351), heartwater with an immunofluorescence antibody test (IFAT) (95.5%, n = 178) and leptospirosis with a Microscopic Agglutination Test (MAT) (25.9%, n = 363). Significant associations were found between seroprevalence to some of the leptospiral serogroups detected (Tarassovi, Pomona, Sejroe and Mini) and age of the animals, animal density or location of the estates (being more prevalent in hotter and more humid areas). In addition, Mycobacterium bovis and M. avium subspecies paratuberculosis were confirmed in two deer carcasses by culture and PCR, respectively. No antibodies against Brucella spp. nor Rift Valley Fever virus were detected with the use of respective indirect ELISA's. The results obtained suggest that the population of rusa deer from Mauritius is exposed to a wide range of pathogens which may affect their productivity. In addition, the results highlight the potential public health risks incurred by deer industry workers and consumers. This survey fills an important gap in knowledge regarding the health of tropical deer meat in Mauritius and justifies the need to implement more regular surveys of selected pathogens in the deer population. © 2013 Blackwell Verlag GmbH.

Lipopolysaccharide Specific Immunochromatography Based Lateral Flow Assay for Serogroup Specific Diagnosis of Leptospirosis in India

PubMed Central

Vanithamani, Shanmugam; Shanmughapriya, Santhanam; Narayanan, Ramasamy; Raja, Veerapandian; Kanagavel, Murugesan; Sivasankari, Karikalacholan; Natarajaseenivasan, Kalimuthusamy

2015-01-01

Background Leptospirosis is a re-emerging infectious disease that is under-recognized due to low-sensitivity and cumbersome serological tests. MAT is the gold standard test and it is the only serogroup specific test used till date. Rapid reliable alternative serogroup specific tests are needed for surveillance studies to identify locally circulating serogroups in the study area. Methods/Principal Findings In the present investigation the serological specificity of leptospiral lipopolysaccharides (LPS) was evaluated by enzyme linked immunosorbent assay (ELISA), dot blot assay and rapid immunochromatography based lateral flow assay (ICG-LFA). Sera samples from 120 MAT positive cases, 174 cases with febrile illness other than leptospirosis, and 121 seronegative healthy controls were evaluated for the diagnostic sensitivity and specificity of the developed assays. LPS was extracted from five locally predominant circulating serogroups including: Australis (27.5%), Autumnalis (11.7%), Ballum (25.8%), Grippotyphosa (12.5%), Pomona (10%) and were used as antigens in the diagnostics to detect IgM antibodies in patients’ sera. The sensitivity observed by IgM ELISA and dot blot assay using various leptospiral LPS was >90% for homologous sera. Except for Ballum LPS, no other LPS showed cross-reactivity to heterologous sera. An attempt was made to develop LPS based ICG-LFA for rapid and sensitive serogroup specific diagnostics of leptospirosis. The developed ICG-LFA showed sensitivity in the range between 93 and 100% for homologous sera. The Wilcoxon analysis showed LPS based ICG-LFA did not differ significantly from the gold standard MAT (P>0.05). Conclusion The application of single array of LPS for serogroup specific diagnosis is first of its kind. The developed assay could potentially be evaluated and employed for as MAT alternative. PMID:26340095

Identification and interspecies transmission of a novel bocaparvovirus among different bat species in China.

PubMed

Lau, Susanna K P; Ahmed, Syed Shakeel; Yeung, Hazel C; Li, Kenneth S M; Fan, Rachel Y Y; Cheng, Toni Y C; Cai, Jian-Piao; Wang, Ming; Zheng, Bo-Jian; Wong, Samson S Y; Woo, Patrick C Y; Yuen, Kwok-Yung

2016-12-01

We report the discovery of a novel bocaparvovirus, bat bocaparvovirus (BtBoV), in one spleen, four respiratory and 61 alimentary samples from bats of six different species belonging to three families, Hipposideridae, Rhinolophidae and Vespertilionidae. BtBoV showed a higher detection rate in alimentary samples of Rhinolophus sinicus (5.7 %) than those of other bat species (0.43-1.59 %), supporting R. sinicus as the primary reservoir and virus spillover to accidental bat species. BtBoV peaked during the lactating season of R. sinicus, and it was more frequently detected among female than male adult bats (P<0.05), and among lactating than non-lactating female bats (P<0.0001). Positive BtBoV detection was associated with lower body weight in lactating bats (P<0.05). Ten nearly complete BtBoV genomes from three bat species revealed a unique large ORF1 spanning NS1 and NP1 in eight genomes and conserved splicing signals leading to multiple proteins, as well as a unique substitution in the conserved replication initiator motif within NS1. BtBoV was phylogenetically distantly related to known bocaparvoviruses with ≤57.3 % genome identities, supporting BtBoV as a novel species. Ms-BtBoV from Miniopterus schreibersii and Hp-BtBoV from Hipposideros pomona demonstrated 97.2-99.9 % genome identities with Rs-BtBoVs from R. sinicus, supporting infection of different bat species by a single BtBoV species. Rs-BtBoV_str15 represents the first bat parvovirus genome with non-coding regions sequenced, which suggested the presence of head-to-tail genomic concatamers or episomal forms of the genome. This study represents the first to describe interspecies transmission in BoVs. The high detection rates in lactating female and juvenile bats suggest possible vertical transmission of BtBoV.

[Prokaryotic expression of trigeminy artificial fusion gene of Leptospira interrogans and the immunogenicity of its products].

PubMed

Luo, Dong-jiao; Qiu, Xiao-feng; Wang, Jiang; Yan, Jin; Wang, Hai-bin; Zhou, Jin-cheng; Yan, Jie

2008-11-01

To construct lipL32/1-lipL21-OmpL1/2 fusion gene of Leptospira interrogans and its prokaryotic expression system, and to identify the immunogenicity of its products. PCR using linking primers was applied to construct lipL32/1-lipL21-OmpL1/2 fusion gene and a prokaryotic expression system of the fusion gene was then established using routine genetic engineering technique. SDS-PAGE was used to examine output of the target recombinant protein rLipL32/1-LipL21-OmpL1/2. Double immunodiffusion and Western Blot assay were applied to identify immunogenicity of rLipL32/1-LipL21-OmpL1/2. lipL32/1-lipL21-OmpL1/2 fusion gene with correct sequence and its prokaryotic expression system E.coli BL21DE3pET42a-lipL32/1-lipL21-ompL1/2 was obtained in this study. The output of rLipL32/1-LipL21- OmpL1/2 after optimisation was 37.78 mg/L. The immunodiffusion titer of rabbit antiserum against rLipL32/1-LipL21-OmpL1/2 was 1:4. The rLipL32/1-LipL21-OmpL1/2 antiserum was able to recognize rLipL32/1-LipL21-OmpL1/2, rLipL32/1, rLipL21 and rOmpL1/2. Positive Western hybridization signals were found among rLipL32/1-LipL21-OmpL1/2 and rabbit antiserum against whole cell of strain 56601 and serum from patients infected with L.interrogans serogroups Icterohaemorrhagiae, Grippotyphosa, Autumnalis and Pomona. The fusion gene lipL32/1-lipL21-OmpL1/2 and its prokaryotic expression system were successfully constructed in this study. The expressed fusion protein can be used as the antigen for developing universal genetic engineering vaccine and universal serological tests of leptospirosis.

[Retrospective seroprevalence study of bovine leptospirosis in Mexico considering the ecological regions].

PubMed

Alvarez, Miguel Angel Luna; Moles y Cervantes, Luis Pedro; Rosas, Dolores Gavaldón; Vasquez, Carmen Nava; García, Félix Salazar

2005-01-01

The newly published information about the different ecological regions of Mexico was analyzed aimed at knowing the situation of bovine leptospirosis. A bibliographical search was made and the articles were chosen according to the following inclusion criteria: a) diagnosis technique: microscopic agglutination, b) positive criterion titres of 1:100 or higher, c) time period: 1991-2003, d) publications such as thesis, memoirs of congresses, non-scientific journals and journals with arbitrage, e) location by states. The duplicated information was considered as the exclusion criteria. The results of frequency and of serovarieties of leptospirosis were reported by state, considering the different ecological regions. Reference to 17 states is made. The arid and semi-arid region had a frequency of 37.8 % with a range from 31% to 59%, the prevalent serovars were H-89 strain (hardjo genotype hardjoprajitno), hardjo, wolffi and tarassovi. In the dry tropical region, there was a frequency of 45.9 % with a range from 27 to 72 %. The prevailing serovarieties were wolffi, hardjo and tarassovi. In the humid tropical region , the frequency was 63.8 % with a range between 31.7 and 84.6 %. The predominating serovarieties were H-89 strain (hardjo genotype hardjoprajitno), hardjo, wolffi and tarassovi. In the mild climate, the average frequency of leptospirosis was 39.4 % with a range from 22.1 to 54.3 %. The prevailing serovarieties were Palo Alto strain (icterohaemorrhagiae), Sinaloa ACR strain (portlandvere), bratislava, pyrogenes, pomona, and H-89 strain (hardjoprajitno), hardjo, wolffi and tarassovi. It was concluded that the presence of antobodies against L. interrogans is endemic in the different ecological regions of Mexico and that there is an elevated prevalence of serovarieties hardjo, wolffi y tarassovi; although in the temperate region, the Palo Alto strain (icterohaemorrhagiae), the Sinaloa ACR strain (portland vere) and Bratislava are present, too. Apparently, the

Prognosis and impact of equine recurrent uveitis.

PubMed

Gerding, J C; Gilger, B C

2016-05-01

Equine recurrent uveitis (ERU) is a leading cause of vision loss in horses. To assess the prognosis and impact of ERU on affected horses and their owners by evaluating the signalment, treatment and outcome (including the loss of use, vision assessment and economic loss). Retrospective impact study. Medical records of horses presenting to the North Carolina State University Veterinary Health Complex (NCSU-VHC) with ERU between 1999 and 2014 were reviewed. Signalment, clinical signs, ophthalmic examination findings, treatments and outcomes were evaluated. Owner questionnaires were completed regarding vision, job/role, monetary value, diagnostic and treatment costs, concurrent illness and outcomes. Records of 224 horses (338 eyes) with ERU were reviewed. There was an overrepresentation of Appaloosas (54; 24.1%), Hanoverians (11; 4.9%) and other Warmbloods (13; 5.8%). Ninety-six eyes (28.4%) were diagnosed as blind and 38 eyes (11.2%) with glaucoma on initial evaluation. Leptospirosis titres of serum and/or aqueous humour were obtained in 88 horses and were positive in 40 horses (45.5%), with L. pomona being the most frequently isolated serovar. Globe loss at the NCSU-VHC occurred in 41 ERU eyes (12.1% of total). Owner questionnaires were evaluated in 194 horses (86.6%) and 91 horses (46.9%) were reported blind in the affected eye(s). Fifty-seven horses (29.4%) did not return to their previous role, while 61 (31.4%) performed at a reduced level. Equine recurrent uveitis decreased the monetary value of 164 horses. Twenty-nine horses (14.9%) were euthanised and 37 (19.1%) underwent change in ownership as a direct result of ERU. The impact of ERU is attributed to the high frequency of blindness, globe loss and loss of function. Euthanasia and change of ownership are common sequelae to the progressive nature of ERU. These factors, along with financial costs of the disease, have a significant impact on affected horses and their owners. © 2015 EVJ Ltd.

Geomorphic evidence of active tectonics in the San Gorgonio Pass region of the San Andreas Fault system: an example of discovery-based research in undergraduate teaching

NASA Astrophysics Data System (ADS)

Reinen, L. A.; Yule, J. D.

2014-12-01

Student-conducted research in courses during the first two undergraduate years can increase learning and improve student self-confidence in scientific study, and is recommended for engaging and retaining students in STEM fields (PCAST, 2012). At Pomona College, incorporating student research throughout the geology curriculum tripled the number of students conducting research prior to their senior year that culminated in a professional conference presentation (Reinen et al., 2006). Here we present an example of discovery-based research in Neotectonics, a second-tier course predominantly enrolling first-and second-year students; describe the steps involved in the four week project; and discuss early outcomes of student confidence, engagement and retention. In the San Gorgonio Pass region (SGPR) in southern California, the San Andreas fault undergoes a transition from predominantly strike-slip to a complex system of faults with significant dip-slip, resulting in diffuse deformation and raising the question of whether a large earthquake on the San Andreas could propagate through the region (Yule, 2009). In spring 2014, seven students in the Neotectonics course conducted original research investigating quantifiable geomorphic evidence of tectonic activity in the SGPR. Students addressed questions of [1] unequal uplift in the San Bernardino Mountains, [2] fault activity indicated by stream knick points, [3] the role of fault style on mountain front sinuosity, and [4] characteristic earthquake slip determined via fault scarp degradation models. Students developed and revised individual projects, collaborated with each other on methods, and presented results in a public forum. A final class day was spent reviewing the projects and planning future research directions. Pre- and post-course surveys show increases in students' self-confidence in the design, implementation, and presentation of original scientific inquiries. 5 of 6 eligible students participated in research the

An Undergraduate Research Experience that Integrates Traditional Field Mapping, LiDAR, and 3D Numerical Modeling: Applying Lessons from a Recent Report from the National Academies of Sciences, Engineering, and Medicine in an Intermediate-Level Tectonic Landscapes Course

NASA Astrophysics Data System (ADS)

Reinen, L. A.; Brenner, K.

2017-12-01

Ongoing efforts to improve undergraduate education in science, technology, engineering, and mathematics (STEM) fields focus on increasing active student participation and decreasing traditional lecture-based teaching. Undergraduate research experiences (UREs), which engage students in the work of STEM professionals, are an example of these efforts. A recent report from the National Academies of Sciences, Engineering and Medicine (Undergraduate Research Experiences for STEM Students: Successes, Challenges, and Opportunities; 2017) provides characteristics of UREs, and indicates that participation in UREs increases student interest and persistence in STEM as well as provides opportunities to broaden student participation in these fields. UREs offer an excellent opportunity to engage students in research using the rapidly evolving technologies used by STEM professionals. In the fall of 2016, students in the Tectonic Landscapes class at Pomona College participated in a course-based URE that combined traditional field mapping methods with analysis of high-resolution topographic data (LiDAR) and 3D numerical modeling to investigate questions of active local faulting. During the first ten weeks students developed skills in: creation of fault maps from both field observations (GPS included) and high-resolution digital elevation models (DEMs), assessment of tectonic activity through analyses of DEMs of hill slope diffusion models and geomorphic indices, and evaluation of fault geometry hypotheses via 3D elastic modeling. Most of these assignments were focused on a single research site. While students primarily used Excel, ArcMap, and Poly3D, no previous knowledge of these was required or assumed. Through this iterative approach, students used increasingly more complex methods as well as gained greater ownership of the research process with time. The course culminated with a 4-week independent research project in which each student investigated a question of their own

Enhancing Human Health Using Space Imagery: Summary of Research

NASA Technical Reports Server (NTRS)

Finarelli, Margaret G.

2002-01-01

The International Space University (ISU) 2002 Summer Session was conducted in Pomona, California, June 29-August 30, 2002. Ninety-nine professionals and students from thirty-one countries attended the Summer Session. More than half of these students participated in the Student Research Design Project entitled, "HI-STAR: Health Improvements through Space Technologies and Resources." ISU's interdisciplinary Student Research Design Projects are intended to have great educational value for the participants and, at the same time, to result in a product that will be useful to the field. The HI-STAR project was a success on both counts. The mission of the ISU students' effort on HI-STAR was to develop and promote a global strategy to help combat malaria using space technology. Like the tiny yet powerful mosquito, HI-STAR is a small program that aspires to make a difference. Timely detection of malaria danger zones is essential to help health authorities and policy makers make decisions about how to manage limited resources for combating malaria. In 2001, the technical support network for prevention and control of malaria epidemics published a study called "Malaria Early Warning Systems: Concepts, Indicators and Partners." This study, funded by Roll Back Malaria, a World Health Organization initiative, offered a framework for a monitoring and early warning system. HI-STAR seeks to build on this proposal and enhance the space elements of the suggested framework. Malaria disease dynamics and distributions are related to environmental variables. From space, environmental conditions that support the growth of mosquito populations can be monitored, Malaria-specific information can be gathered from satellite-borne remote sensing instruments and ground-based sensors. This information can be integrated via geographic information systems (GIS) into a Malaria Information System (MIS) that can provide assessment analyses and risk maps as output. HI-STAR defines and suggests the

Shedding light on the subject: introduction to illumination engineering and design for multidiscipline engineering students

NASA Astrophysics Data System (ADS)

Ronen, Ram S.; Smith, R. Frank

1995-10-01

Educating engineers and architects in Illumination Engineering and related subjects has become a very important field and a very satisfying and rewarding one. Main reasons include the need to significantly conserve lighting energy and meet government regulations while supplying appropriate light levels and achieving aesthetical requirements. The proliferation of new lamps, luminaries and lighting controllers many of which are 'energy savers' also helps a trend to seek help from lighting engineers when designing new commercial and residential buildings. That trend is believed to continue and grow as benefits become attractive and new government conservation regulations take affect. To make things even better one notices that Engineering and Science students in most disciplines make excellent candidates for Illumination Engineers because of their background and teaching them can move ahead at a brisk pace and be a rewarding experience nevertheless. In the past two years, Cal Poly Pomona College of Engineering has been the beneficiary of a DOE/California grant. Its purpose was to precipitate and oversee light curricula in various California community colleges and also develop and launch an Illumination Engineering minor at Cal Poly University. Both objectives have successfully been met. Numerous community colleges throughout California developed and are offering a sequence of six lighting courses leading to a certificate; the first graduating class is now coming out of both Cypress and Consumnes Community Colleges. At Cal Poly University a four course/laboratory sequence leading to a minor in Illumination Engineering (ILE) is now offered to upper division students in the College of Engineering, College of Science and College of Architecture and Design. The ILE sequence will briefly be described. The first course, Introduction to Illumination Engineering and its laboratory are described in more detail alter. Various methods of instruction including lectures, self work

A Remotely Sensed and Paleomagnetic Perspective on the Bonelli Tuff of NW AZ and SE CA

NASA Astrophysics Data System (ADS)

Gomez, C. D.

2015-12-01

The southern Black and Cerbat Mountains of NW AZ and the Sacramento Mountains of SE CA preserve ignimbrites associated with multiple episodes of volcanic activity that span at least a million years. Unraveling the stratrigraphy of these deposits, as well as their eruptive centers, is critical for constraining the volcanic history of this ignimbrite, the 18.8 Ma Peach Spring Tuff, is the recently identified 17.7 Ma Tuff of Bonelli House (TB) (Ferguson & Cook 2015) and may also occur in the southern Black and Sacramento Mountains. To help determine the extent and possible source of the TB, we have performed a combined remote sensing and paleomagnetic study of this unit, including possible correlatives. Paleomagnetic work involved Remanence and anisotropic magnetic susceptibility methods. Drill samples were collected and processed at Scripps Institute of Oceanography & Pomona College. An AC current was run to obtain the Paleomag current, as opposed to the traditional of heating up the cores at specific intervals. Sacramento Mountains samples produced an average direction of 200.9 / -26.4, which contrasts the Peach Spring Tuff paleodirection of 036.4/33 (Wells & Hillhouse, 1989). An AMS direction was determined using a MFK1 Kappabridge instrument and consistently showed similar flow direction to that of the PST. In compiling our data on a map, we took into account the Whipple Detachment Fault, ~40 km westward (Lister & Davis, 1989). We were able to identify a spectral signature and remnant paleomagnetic direction for the TB and identify potential additional outcrops in the southern Black mountains. AMS showed us that the ignimbrites originated from a source in the Silver Creek Caldera, which may indicate the PST at TB were produced from a similar source. The remnant paleomagnetic direction allows us to closely correlate these tuff units as occurring within a similar timeframe. The contrasting paleodirection of the TB and the PST allows us to confidently say that the

Life in the Universe - Astronomy and Planetary Science Research Experience for Undergraduates at the SETI Institute

NASA Astrophysics Data System (ADS)

Chiar, J.; Phillips, C. B.; Rudolph, A.; Bonaccorsi, R.; Tarter, J.; Harp, G.; Caldwell, D. A.; DeVore, E. K.

2016-12-01

The SETI Institute hosts an Astrobiology Research Experience for Undergraduates (REU) program. Beginning in 2013, we partnered with the Physics and Astronomy Dept. at Cal Poly Pomona, a Hispanic-serving university, to recruit underserved students. Over 11 years, we have served 155 students. We focus on Astrobiology since the Institute's mission is to explore, understand and explain the origin, nature and prevalence of life in the universe. Our REU students work with mentors at the Institute - a non-profit organization located in California's Silicon Valley-and at the nearby NASA Ames Research Center. Projects span research on survival of microbes under extreme conditions, planetary geology, astronomy, the Search for Extraterrestrial Intelligence (SETI), extrasolar planets and more. The REU program begins with an introductory lectures by Institute scientists covering the diverse astrobiology subfields. A week-long field trip to the SETI Institute's Allen Telescope Array (Hat Creek Radio Astronomy Observatory in Northern California) and field experiences at hydrothermal systems at nearby Lassen Volcanic National Park immerses students in radio astronomy and SETI, and extremophile environments that are research sites for astrobiologists. Field trips expose students to diverse environments and allow them to investigate planetary analogs as our scientists do. Students also participate in local trips to the California Academy of Sciences and other nearby locations of scientific interest, and attend the weekly scientific colloquium hosted by the SETI Institute at Microsoft, other seminars and lectures at SETI Institute and NASA Ames. The students meet and present at a weekly journal club where they hone their presentation skills, as well as share their research progress. At the end of the summer, the REU interns present their research projects at a session of the Institute's colloquium. As a final project, students prepare a 2-page formal abstract and 15-minute

Use of the SMART Balance Master to quantify the effects of osteopathic manipulative treatment in patients with dizziness.

PubMed

Fraix, Marcel; Gordon, Ashlynn; Graham, Victoria; Hurwitz, Eric; Seffinger, Michael A

2013-05-01

Dizziness is the third most common complaint among outpatients and the most common complaint in patients aged 75 years or older. It can be incapacitating for patients, affecting both productivity and quality of life. To evaluate the effect of osteopathic manipulative treatment (OMT) for spinal somatic dysfunction in patients with dizziness lasting longer than 3 months. A prospective clinical cohort study that took place in 2011. Department of Physical Therapy laboratory at the Western University of Health Sciences College of Osteopathic Medicine in Pomona, California. Sixteen participants (2 male, 14 female; mean [range] age, 49 [13-75] years) with dizziness lasting at least 3 months (mean duration of symptoms, 84 months) and spinal somatic dysfunction, but no history of known stroke or brain disease, were recruited from the local community and evaluated for postural balance control before, immediately after, and 1 week after OMT. Four osteopathic physicians board certified in neuromusculoskeletal medicine/osteopathic manipulative medicine provided OMT, including muscle energy; high-velocity, low-amplitude; counterstrain; myofascial release; balanced ligamentous release; and cranial OMT techniques. Outcomes were assessed with the SMART Balance Master (NeuroCom), a validated instrument that provides graphic and quantitative analyses of sway and balance, and the Dizziness Handicap Inventory (DHI), a self-assessment inventory designed to assess precipitating physical factors associated with dizziness and functional and emotional consequences of vestibular disease. Paired t tests, performed to assess changes in mean composite scores for all challenge tests, revealed that balance was significantly improved both immediately and 1 week after OMT (both P<.001), with no significant difference between immediate and 1-week post-OMT scores (P=.20). The DHI scores, both total and subscale, improved significantly after OMT (P<.001), and changes in composite and DHI scores were

Changing trends in serotypes of S. pneumoniae isolates causing invasive and non-invasive diseases in unvaccinated population in Mexico (2000-2014).

PubMed

Carnalla-Barajas, María Noemí; Soto-Noguerón, Araceli; Sánchez-Alemán, Miguel Angel; Solórzano-Santos, Fortino; Velazquez-Meza, María Elena; Echániz-Aviles, Gabriela

2017-05-01

Introduction of pneumococcal conjugate vaccines (PCV) targeted against a limited number of serotypes substantially decreased invasive (IPD) and non-invasive pneumococcal diseases (NIPD) but it was accompanied by non-vaccine type replacement disease. After 9 years of introduction of PCV in Mexico, we analyze the evidence of the indirect effects on IPD and NIPD serotype distribution among groups not targeted to receive the vaccine. From January 2000 to December 2014, pneumococcal strains isolated from IPD and NIPD cases from patients ≥5 years of age from participant hospitals of the SIREVA II (Sistema Regional de Vacunas) network were serotyped. A regression analysis was performed considering year and proportion of serotypes included in the different vaccine formulations (PCV7, PCV10 and PCV13). The slope was obtained for each regression line and their correspondent p-value. The proportion of each serotype in the pre-PCV7 and post-PCV7 periods was evaluated by χ2 test. From a total of 1147 pneumococcal strains recovered, 570 corresponded to the pre-PCV7 and 577 to the post-PCV7 periods. The proportion of vaccine serotypes included in the three PCV formulations decreased by 2.4, 2.6 and 1.3%, respectively per year during the study period. A significant increase of serotype 19A was observed in the post-vaccine period in all age groups. A percentage of annual decline of serotypes causing IPD and NIPD included in PCV was detected among groups not targeted to receive the vaccine, probably due to herd effect. Considering pneumococcal serotype distribution is a dynamic process, we highlight the importance of surveillance programs. Copyright © 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.

Efficacy of a New Recrystallized Enrofloxacin Hydrochloride-Dihydrate against Leptospirosis in a Hamster Model.

PubMed

Carrascosa, Alma; Gutierrez, Lilia; De la Peña, Alejandro; Candanosa, Irma E; Tapia, Graciela; Sumano, Hector

2017-11-01

A trial on Syrian hamsters ( Mesocricetus auratus ) infected with Leptospira interrogans serovar Canicola was established to compare treatment efficacies of daily intramuscular (i.m.) injections of either 10 mg/kg of 5% enrofloxacin (Baytril [BE]; Bayer Animal Health, Mexico) or the same dose of enrofloxacin hydrochloride-dihydrate (enro-C). Hamsters were experimentally infected via the oral submucosa with 400 microorganisms/animal, in a sequential time schedule aligned to the initial treatment day, and were treated in groups as follows: a group treated with 5% enrofloxacin daily for 7 days after 24 h of infection (group BE 24 ); a group treated as described for group BE 24 but with enro-C (enro-C 24 ); a group also treated with 5% enrofloxacin but starting at 72 h after infection (BE 74 ); a group treated as described for group BE 74 but with injection of enro-C (enro-C 74 ). An untreated-uninfected control group (group CG - ) and an infected-untreated control group (group CG + ) were assembled ( n = 18 in all groups). Weights and temperatures of the hamsters were monitored daily for 28 days. After hamsters were euthanatized or following death, necropsy, histopathology, macroscopic agglutination tests (MAT), bacterial culture, and PCR were performed. The mortality rates were 38.8% in group BE 24 and 100% in group BE 74 No mortality was observed in group enro-C 24 , and 11.1% mortality was recorded in group enro-C 74 The mortality rates in groups CG + and CG - were 100% and zero, respectively. Combined necropsy and histopathologic findings revealed signs of septicemia and organ damage in groups BE 24 , BE 72 , and CG + Groups enro-C 24 and CG - showed no lesions. Moderated lesions were registered in 3 hamsters in group enro-C 72 MAT results were positive in 83.3% of BE 24 hamsters (83.3%) and 100% of BE 72 and CG + hamsters; MAT results were positive in 16.7% in group Enro-C 24 and 38.9% in group enro-C 72 Only 4/18 were PCR positive in group enro-C 72 and only 1

Chemists, Access, Statistics

NASA Astrophysics Data System (ADS)

Holmes, Jon L.

2000-06-01

IP-number access. Current subscriptions can be upgraded to IP-number access at little additional cost. We are pleased to be able to offer to institutions and libraries this convenient mode of access to subscriber only resources at JCE Online. JCE Online Usage Statistics We are continually amazed by the activity at JCE Online. So far, the year 2000 has shown a marked increase. Given the phenomenal overall growth of the Internet, perhaps our surprise is not warranted. However, during the months of January and February 2000, over 38,000 visitors requested over 275,000 pages. This is a monthly increase of over 33% from the October-December 1999 levels. It is good to know that people are visiting, but we would very much like to know what you would most like to see at JCE Online. Please send your suggestions to JCEOnline@chem.wisc.edu. For those who are interested, JCE Online year-to-date statistics are available. Biographical Snapshots of Famous Chemists: Mission Statement Feature Editor: Barbara Burke Chemistry Department, California State Polytechnic University-Pomona, Pomona, CA 91768 phone: 909/869-3664 fax: 909/869-4616 email: baburke@csupomona.edu The primary goal of this JCE Internet column is to provide information about chemists who have made important contributions to chemistry. For each chemist, there is a short biographical "snapshot" that provides basic information about the person's chemical work, gender, ethnicity, and cultural background. Each snapshot includes links to related websites and to a biobibliographic database. The database provides references for the individual and can be searched through key words listed at the end of each snapshot. All students, not just science majors, need to understand science as it really is: an exciting, challenging, human, and creative way of learning about our natural world. Investigating the life experiences of chemists can provide a means for students to gain a more realistic view of chemistry. In addition students

Detection and genome characterization of four novel bat hepadnaviruses and a hepevirus in China.

PubMed

Wang, Bo; Yang, Xing-Lou; Li, Wen; Zhu, Yan; Ge, Xing-Yi; Zhang, Li-Biao; Zhang, Yun-Zhi; Bock, Claus-Thomas; Shi, Zheng-Li

2017-02-22

In recent years, novel hepadnaviruses, hepeviruses, hepatoviruses, and hepaciviruses have been discovered in various species of bat around the world, indicating that bats may act as natural reservoirs for these hepatitis viruses. In order to further assess the distribution of hepatitis viruses in bat populations in China, we tested the presence of these hepatitis viruses in our archived bat liver samples that originated from several bat species and various geographical regions in China. A total of 78 bat liver samples (involving two families, five genera, and 17 species of bat) were examined using nested or heminested reverse transcription PCR (RT-PCR) with degenerate primers. Full-length genomic sequences of two virus strains were sequenced followed by phylogenetic analyses. Four samples were positive for hepadnavirus, only one was positive for hepevirus, and none of the samples were positive for hepatovirus or hepacivirus. The hepadnaviruses were discovered in the horseshoe bats, Rhinolophus sinicus and Rhinolophus affinis, and the hepevirus was found in the whiskered bat Myotis davidii. The full-length genomic sequences were determined for one of the two hepadnaviruses identified in R. sinicus (designated BtHBVRs3364) and the hepevirus (designated BtHEVMd2350). A sequence identity analysis indicated that BtHBVRs3364 had the highest degree of identity with a previously reported hepadnavirus from the roundleaf bat, Hipposideros pomona, from China, and BtHEVMd2350 had the highest degree of identity with a hepevirus found in the serotine bat, Eptesicus serotinus, from Germany, but it exhibited high levels of divergence at both the nucleotide and the amino acid levels. This is the first study to report that the Chinese horseshoe bat and the Chinese whiskered bat have been found to carry novel hepadnaviruses and a novel hepevirus, respectively. The discovery of BtHBVRs3364 further supports the significance of host switches evolution while opposing the co

Circulating serovars of Leptospira in cart horses of central and southern Ethiopia and associated risk factors.

PubMed

Tsegay, K; Potts, A D; Aklilu, N; Lötter, C; Gummow, B

2016-03-01

Little work has been done on diseases of horses in Ethiopia or tropical regions of the world. Yet, Ethiopia has the largest horse population in Africa and their horses play a pivotal role in their economy as traction animals. A serological and questionnaire survey was therefore conducted to determine the circulating serovars of Leptospira and their association with potential risk factors in the cart horse population of Central and Southern Ethiopia. A total of 184 out of 418 cart horses from 13 districts had antibody titres of 1:100 or greater to at least one of 16 serovars of Leptospira species in Central and Southern Ethiopian horses. A significantly higher seropositivity (62.1%) was noted in horses from the highland agroecology followed by midland (44.4%) and lowland (39.8%). Serovar Bratislava (34.5%) was the predominant serovar followed by serovars Djasiman (9.8%), Topaz (5.98%) and Pomona (5.3%). Age and location proved to be associated with seropositive horses with older horses being more commonly affected and the districts of Ziway (Batu) (Apparent Prevalence (AP)=65.5%), Shashemene (AP=48.3%) and Sebeta (AP=41.4%) having the highest prevalence. Multivariable logistic regression found risk factors significantly associated with Leptospira seropositive horses were drinking river water (OR=2.8) and horses 7-12 years old (OR=5) and risk factors specifically associated with serovar Bratislava seropositive horses were drinking river water (OR=2.5), horses ≥13 years (OR=3.5) and the presence of dogs in adjacent neighbouring properties (OR=0.3). Dogs had a protective effect against seropositivity to serovars Bratislava and Djasiman, which may be due to their ability to control rodents. The high seroprevalence confirm that leptospirosis is endemic among horses of Central and Southern Ethiopia. The predominance of serovar Bratislava supports the idea that serovar Bratislava may be adapted to and maintained by the horse population of Central and Southern Ethiopia

Risk factors and topographies for self-injurious behaviour in a sample of adults with intellectual developmental disorders.

PubMed

Folch, A; Cortés, M J; Salvador-Carulla, L; Vicens, P; Irazábal, M; Muñoz, S; Rovira, L; Orejuela, C; Haro, J M; Vilella, E; Martínez-Leal, R

2018-04-01

Self-injurious behaviour (SIB) is a prevalent form of challenging behaviour in people with intellectual developmental disorders (IDD). Existing research has yielded conflicting findings concerning the major risk factors involved, and in addition, SIB shows multiple topographies and presentations. Although presence of autism spectrum disorders (ASD) and severity of intellectual disability (ID) are known risk factors for SIB, there are no studies comparing SIB topographies by severity degrees of ID and ASD. The purpose of the present paper has been to identify risk factors and topographies for SIB in a representative, stratified and randomised sample of adults with IDD. This study was conducted on the basis of data collected by the POMONA-ESP project, in a sample of 833 adults with IDD. Data concerning demographic and health information, ASD symptoms, psychopathology and ID, have been analysed to determine the presence of risk factors for SIB among participants and to explore the occurrence and topographies of SIB across different severity levels of ID and ASD symptoms. Self-injurious behaviour prevalence in the sample was 16.2%. Younger age, oral pain, greater severity of ID, presence of dual diagnosis, psychiatric medication intake and higher scores on Childhood Autism Rating Scale were risk factors for SIB among participants, whereas number of areas with functioning limitations, place of residence, diagnosis of epilepsy and sex were not. SIB was more frequent in participants with ASD symptoms regardless of its severity level, and they displayed a higher number of different topographies of SIB. People with profound ID without co-morbid ASD symptoms showed similar results concerning SIB prevalence and topographies. Knowledge on risk factors and topographies of SIB might play a vital role in the development of prevention strategies and management of SIB in people with IDD. The mere presence of ASD symptoms, regardless of its severity level, can be a crucial factor to

Simultaneous analysis of psychotropic phenylalkylamines in oral fluid by GC-MS with automated SPE and its application to legal cases.

PubMed

Choi, Hyeyoung; Baeck, Seungkyung; Jang, Moonhee; Lee, Sooyeun; Choi, Hwakyung; Chung, Heesun

2012-02-10

Phenylalkylamine derivatives, such as methamphetamine (MA), amphetamine (AM), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), phentermine (PT), fenfluramine (FFA) and phenmetrazine (PM), and ketamine (KT) are widely abused recreational or anorectic drugs in Korea and are regulated under the Controlled Substance Act in Korea. Phenylalkylamines and ketamine analysis is normally performed using both urine and hair samples but there is no established method for the simultaneous analysis of all these phenylalkylamines and ketamine in oral fluids. Oral fluid is easy to collect/handle and can provide an indication of recent drug abuse. In this study, to confirm the presence of phenylalkylamine derivatives and ketamine in oral fluid after screening with an immunoassay, an analytical method using automated solid phase extraction (SPE) and gas chromatography-mass spectrometry (GC-MS) was developed and fully validated according to international guidelines. The applicability of the assay was demonstrated by analyzing of authentic oral fluid samples and the results of oral fluid analysis were compared with those in urine and hair to to evaluate the feasibility of oral fluid in forensic cases. The recovery of phenylalkylamines and ketamine from oral fluid collection devices was also assessed. Oral fluid specimens from 23 drug abuse suspects submitted by the police were collected using Salivette (Sarstedt, Nümbrecht, Germany), Quantisal (Immunalysis, Pomona, CA) or direct expectoration. The samples were screened using a biochip array analyzer (Evidence Investigator, Randox, Antrim, UK). For confirmation, the samples were analyzed by GC-MS in selected-ion monitoring (SIM) mode after extraction using automated SPE (RapidTrace, Zymark, MA, USA) with a mixed-mode cation exchange cartridge (CLEAN SCREEN, 130 mg/3 ml, UCT, PA, USA) and derivatization with trifluoroacetic anhydride (TFA). The results from the immunoassay were consistent with those from GC

Assessment of Research Interests of First-Year Osteopathic Medical Students.

PubMed

Carter, John; McClellan, Nicholas; McFaul, Derek; Massey, Blaine; Guenther, Elisabeth; Kisby, Glen

2016-07-01

According to a 2014 survey, 59% of students entering allopathic medical school reported previous research experience. However, limited data exist on the amount of research experience that students have before entering osteopathic medical school. A strong understanding of the research skills and level of interest of first-year osteopathic medical students is essential for developing research programs at osteopathic medical schools. Limited data exist on the amount of research experience that students have before starting osteopathic medical school. A strong understanding of the research skills and level of interest of first-year medical students is essential for developing research programs at osteopathic medical schools. To determine the amount of previous research experience of first-year osteopathic medical students, their level of interest in participating in research during medical school, the factors influencing their interest in research, and their research fields of interest. First-year osteopathic medical students (class of 2019) at the Western University of Health Sciences, College of Osteopathic Medicine of the Pacific in Pomona, California (WesternU/COMP), and Pacific-Northwest in Lebanon, Oregon (WesternU/COMP-Northwest), campuses were surveyed about their previous research experiences and whether they were interested in participating in research during medical school. Surveys were administered through an anonymous online portal. Responses were evaluated for evidence of interest in conducting research. Of the 346 osteopathic medical students invited to participate in the study, the response rate was 77% (N=266). A total of 167 from WesternU/COMP and 99 from the WesternU/COMP-Northwest responded. More than 215 students (81%) reported they had participated in research before entering medical school. In addition, 200 students (75%) either expressed a strong interest in participating in research during medical school or were currently conducting research

Cases of Coastal Zone Change and Land Use/Land Cover Change: a learning module that goes beyond the "how" of doing image processing and change detection to asking the "why" about what are the "driving forces" of global change.

NASA Astrophysics Data System (ADS)

Ford, R. E.

2006-12-01

In 2006 the Loma Linda University ESSE21 Mesoamerican Project (Earth System Science Education for the 21st Century) along with partners such as the University of Redlands and California State University, Pomona, produced an online learning module that is designed to help students learn critical remote sensing skills-- specifically: ecosystem characterization, i.e. doing a supervised or unsupervised classification of satellite imagery in a tropical coastal environment. And, it would teach how to measure land use / land cover change (LULC) over time and then encourage students to use that data to assess the Human Dimensions of Global Change (HDGC). Specific objectives include: 1. Learn where to find remote sensing data and practice downloading, pre-processing, and "cleaning" the data for image analysis. 2. Use Leica-Geosystems ERDAS Imagine or IDRISI Kilimanjaro to analyze and display the data. 3. Do an unsupervised classification of a LANDSAT image of a protected area in Honduras, i.e. Cuero y Salado, Pico Bonito, or Isla del Tigre. 4. Virtually participate in a ground-validation exercise that would allow one to re-classify the image into a supervised classification using the FAO Global Land Cover Network (GLCN) classification system. 5. Learn more about each protected area's landscape, history, livelihood patterns and "sustainability" issues via virtual online tours that provide ground and space photos of different sites. This will help students in identifying potential "training sites" for doing a supervised classification. 6. Study other global, US, Canadian, and European land use/land cover classification systems and compare their advantages and disadvantages over the FAO/GLCN system. 7. Learn to appreciate the advantages and disadvantages of existing LULC classification schemes and adapt them to local-level user needs. 8. Carry out a change detection exercise that shows how land use and/or land cover has changed over time for the protected area of your choice

A cluster of three cases of leptospirosis in dairy farm workers in New Zealand.

PubMed

McLean, Margot; Ruscoe, Quentin; Kline, Terence; King, Caleb; Nesdale, Annette

2014-01-24

We report a cluster of three cases of leptospirosis on a New Zealand dairy farm, with regard to clinical, laboratory, and environmental findings. The cluster is discussed against the annual incidence of leptospirosis in humans and cattle, and the vaccination of cattle as one means of preventing human cases on farms. The three cases were investigated by case interview and review of clinical and laboratory information. A site visit was made to the farm to assess environmental risk. Relevant veterinary information relating to the cattle herds was reviewed. Most of the symptoms exhibited by the three patients were consistent with primary phase leptospirosis. Different methods of laboratory diagnosis were used with each case. However, two cases were confirmed as leptospirosis and in both the causative agent was Leptospira borgpetersenii serovar (sv) Hardjo. The third case had a milder illness, received doxycycline early, and was regarded as a 'probable' case as there were no confirmatory diagnostic results. All three cases had worked on the same dairy farm during their incubation period, where the highest risk environment was the milking shed and potential exposure to urine splashes from infected cattle. Also there were inadequacies in the herd vaccination programme. There are options for minimising risk to dairy farm workers in New Zealand. No human vaccine exists in this country. Leptospira borgpetersenii serovar (sv) Hardjo (serovar Hardjo) is endemic in New Zealand dairy cattle without causing apparent disease. L. Pomona is a sporadic infection but can cause abortions. A cattle vaccine against these serovars was introduced in New Zealand in 1979, after which there was a general fall in notifications of human cases of leptospirosis. This was attributed to the overall decrease in these two serovars among the livestock population. Vaccination of farm livestock for leptospirosis is an integral factor in preventing human cases. We note the New Zealand initiative to

Paleomagnetism of the Miocene Columbia River Basalt Group in Oregon and Washington from the Pacific Coast to the Columbia Plateau: Magnetostratigraphy, Vertical-Axis Rotations, Paleosecular Variation, and Remagnetization

NASA Astrophysics Data System (ADS)

Hagstrum, J. T.; Wells, R. E.; Evarts, R. C.; Niem, A. R.; Sawlan, M. G.; Blakely, R. J.

2008-12-01

Identification of individual flows within the Columbia River Basalt Group (CRBG) has mostly relied on minor differences in geochemistry, but magnetic polarity has also proved useful in differentiating flows and establishing a temporal framework. Within the thick, rapidly erupted Grande Ronde Basalt four major polarity chrons (R1 to N2) have been identified. Because cooling times of CRBG flows are brief compared to rates of paleosecular variation (PSV), within-flow paleomagnetic directions are expected to be constant across the extensive east-west reaches of these flows. Vertical-axis rotations in OR and WA, driven by northward-oblique subduction of the Juan de Fuca plate, thus can be measured by comparing directions for western sampling localities to directions for the same flow units on the relatively stable Columbia Plateau. Clockwise rotations calculated for outcrop locations within the Coast Range (CR) block are uniformly about 30° (N=102 sites). East of the northwest-trending en échelon Mt. Angel-Gales Creek, Portland Hills, and northern unnamed fault zones, as well as north of the CR block's northern boundary (~Columbia River), clockwise rotations abruptly drop to about 15° (N=39 sites), with offsets in these bounding fault zones corresponding to the Portland and Willamette pull-apart basins. The general agreement of vertical- axis rotation rates estimated from CRBG magnetizations with those determined from modern GPS velocities indicates a relatively steady rate over the last 10 to 15 Myr. Unusual directions due to PSV, field excursions, or polarity transitions could provide useful stratigraphic markers. Individual flow directions, however, have not been routinely used to identify flows. One reason this has been difficult is that remagnetization is prevalent, particularly in the Coast Ranges, coupled with earlier demagnetization techniques that did not completely remove overprint components. Except for the Ginkgo and Pomona flows of the Wanapum and Saddle

Laboratory-based educational and outreach activities in the framework of a CAREER award at the University of Oregon

NASA Astrophysics Data System (ADS)

Bindeman, I. N.

2011-12-01

The Stable Isotope Laboratory at the University of Oregon has been used as a learning and outreach center in the framework of the 09 award entitled "Stable isotope insights into large-volume volcanic eruptions". The PI and other members of the group have actively recruitted undergraduate students, summer session and catalytic outreach undergraduates, and hosted international students, visitors, and collaborators from Russia, Iceland, France, the UK, Australia, and Switzerland. We also integrated closely with the Oregon-wide summer program that brings community college students to the University of Oregon for 2.5 months summer research residence (UCORE). In total we gave supervised five undergraduate students and three UCORE students. Additionally, we recruited undergraduates from U of Chicago, Colorado and Pomona Colleges to spend summers in the lab and in the field. In conjunction with the NSF funded PIRE program, two female graduate and one female undergraduate students participated in fieldwork in Kamchatka, and three Kamchatka undergraduates, and one Moscow graduate student visited the University Oregon. Students performed their own projects or Senior Theses and reported their results locally and at AGU conferences. We developed a management structure in which graduate students, a postdoc, and lab technician co-supervised students and visitors and this exposed them into the supervisory roles, contributed to the project progress, and liberated PI from micromanagement duties. The talk will present our experience with this management concept of a lab-based-learning initiative, which defines roles for each member of the lab. Our outreach activities included public lectures at community colleges by PI and a graduate student, and the topical Penrose conference co-organized by the PI, which attracted many students and visitors who collected their data in the lab. PI has introduced a voluntary fieldtrip as a part of his Volcanoes and Earthquake large enrollment class

Estimación de la Relación Costo-Efectividad de las Vacunas Neumocócicas Conjugadas Prevenar-13 y Synflorix®, Utilizadas en Los Programas de Vacunación de Población Infantil Mexicana.

PubMed

Gómez, Jorge A; Villaseñor-Sierra, Alberto; Aguilar, Gerardo Martínez; Manjarrez, Roberto Carreño; Cervantes-Apolinar, María Y

2016-12-01

To estimate the cost effectiveness associated with the use of pneumococcal conjugated vaccines, Prevenar-13 and Synflorix®, in the Mexican pediatric population. The cost-effectiveness ratio of instrumenting vaccination programs based upon the use of Prevenar-13 and Synflorix® in the Mexican pediatric population was estimated by using a Markov's simulation model. The robustness of the conclusions reached on cost-effectiveness for both vaccines was assayed through an univariate and probabilistic sensitivity analysis that included all of the parameters considered by the model. Synflorix® was dominant over Prevenar-13 in the cost-utility analysis; the former generated more quality-adjusted life years at a lower cost and with a lower incremental cost-utility ratio. Based on the cost-effective analysis, Prevenar-13 generated more life years gained but at a higher cost. The use of Prevenar-13 originated a higher incremental cost-effectiveness ratio and, therefore, it was not cost-effective as compared with Synflorix®. Even though the simulations for Prevenar-13 and Synflorix® revealed both of them to be cost-effective when used to instrument pediatric vaccination campaigns in Mexico, Synflorix® had a better cost-utility/effectiveness profile. In addition, although Prevenar-13 and Synflorix® produced equivalent health outcomes, the overall analysis predicted that Synflorix® would save 360 million Mexican pesos, as compared with Prevenar-13. Copyright © 2016 International Society for Pharmacoeconomics and Outcomes Research (ISPOR). Published by Elsevier Inc. All rights reserved.

Aftershocks and triggered events of the Great 1906 California earthquake

USGS Publications Warehouse

Meltzner, A.J.; Wald, D.J.

2003-01-01

The San Andreas fault is the longest fault in California and one of the longest strike-slip faults in the world, yet little is known about the aftershocks following the most recent great event on the San Andreas, the Mw 7.8 San Francisco earthquake on 18 April 1906. We conducted a study to locate and to estimate magnitudes for the largest aftershocks and triggered events of this earthquake. We examined existing catalogs and historical documents for the period April 1906 to December 1907, compiling data on the first 20 months of the aftershock sequence. We grouped felt reports temporally and assigned modified Mercalli intensities for the larger events based on the descriptions judged to be the most reliable. For onshore and near-shore events, a grid-search algorithm (derived from empirical analysis of modern earthquakes) was used to find the epicentral location and magnitude most consistent with the assigned intensities. For one event identified as far offshore, the event's intensity distribution was compared with those of modern events, in order to contrain the event's location and magnitude. The largest aftershock within the study period, an M ???6.7 event, occurred ???100 km west of Eureka on 23 April 1906. Although not within our study period, another M ???6.7 aftershock occurred near Cape Mendocino on 28 October 1909. Other significant aftershocks included an M ???5.6 event near San Juan Bautista on 17 May 1906 and an M ???6.3 event near Shelter Cove on 11 August 1907. An M ???4.9 aftershock occurred on the creeping segment of the San Andreas fault (southeast of the mainshock rupture) on 6 July 1906. The 1906 San Francisco earthquake also triggered events in southern California (including separate events in or near the Imperial Valley, the Pomona Valley, and Santa Monica Bay), in western Nevada, in southern central Oregon, and in western Arizona, all within 2 days of the mainshock. Of these trigerred events, the largest were an M ???6.1 earthquake near Brawley

Can Skateboarding Save the Planet? A Curricular Unit on Global Climate Change Developed Through the NASA LIFT-OFF Program

NASA Astrophysics Data System (ADS)

Pruett, L. E.; Burrell, S.; Chidester, C.; Metzger, E. P.

2010-12-01

The inclusion of global climate change education in California public high schools is constrained by several factors, including the planning time needed to effectively correlate state content standards to the multidisciplinary science of climate change, the lack of time in the curriculum, and budget constraints that limit resources for teachers. Recent efforts by the NASA LIFT-OFF program to support classroom teachers in the development of inquiry-based curricular materials have helped to alleviate many of these burdens. NASA LIFT-OFF is funded by a grant to the Alameda County Office of Education and involves a partnership between the Alameda, Santa Clara, and Los Angeles county offices of education and science faculty at California State University (CSU) East Bay, San Jose State University (SJSU), and Cal Poly Pomona. LIFT-OFF goals are to improve high school science teachers’ content knowledge through interactions with scientists from the CSU campuses, NASA, and the SETI Institute and to enhance their ability to plan and implement high-quality science inquiry in their classrooms. LIFT-OFF teachers at the three CSU campuses are developing instructional cases that use NASA resources and research-based pedagogical practices to explore engaging real-world questions. We participated in SJSU’s 2010 LIFT-OFF summer institute and worked as a team to develop a 12-day unit for high school students that focuses on the science behind global climate change. In addition to delivering science content, the unit engages students in critical thinking and evaluation. Students generate, access and interpret data, and use the knowledge gained to make small lifestyle changes that aid in the reduction of their greenhouse gas emissions. Not only does this unit of study empower students to make science-based decisions, it also incorporates diverse learning strategies, such as the use of visuals aids, language acquisition techniques to improve literacy, formative assessments and daily

The warm chain.

PubMed

Bowers, J Z

1985-01-01

1 series of events carried smallpox vaccination on an eastward odyssey; a 2nd path led westward from Spain to Spanish America, to the Philippines, and to China. Francisco Xavier Balmis (1753-1819), a Spanish physician, sailed around the world in 3 years, establishing vaccination boards in South America, the Philippines, and China. He led the "Real Expedicion Martima de la Vacuna," sponsored by the Bourbon King Charles IV; its success derived from arm-to-arm passage of the virus using orphan boys on the long voyages across the Atlantic and Pacific oceans. Venezuela was the 1st country to which Balmis introduced vaccine. He divided the expedition with his deputy, Slavany, who was to lead 1 group through the Spanish colonies in the Vice-Royalty of Peru; Balmis would lead the other group across the Vice-Royalty of New Spain to the far-distant colony in the Philippines. The establishment of central boards to regulate vaccination became one of Balmis's principal preoccupations. The board he created in Venezuela served as the model for those that he and Salvany were to establish later. Balmis sailed to Havana from La Guaira on May 8, 1804; Salvany led his team to Bogota. Balmis's singular success in Caracas, and Salvany's in Bogota, where Salvany also created a Central Vaccination Board, were attributable in large measure to the total support they received from church and civil authorites. The Portuguese throne had a comparable interest in its prize colony, Brazil. In 1804, Felisberto Calderia Brant Pontes sent a group of slave children from Bahia to Lisbon so that arm-to-arm transfer could be made on the return voyage. His effort was successful, and 1335 persons had been vaccinated in Bahia by June 1, 1804. In a decree of September 3, 1803 Charles IV directed that the Balmis expedition should continue to the Philippines. The advancement of health in the Philippines was a major commitment of the Spaniards. The Philippine vaccination expedition, headed by Balmis, sailed

Cosmic Blasts Much More Common, Astronomers Discover

NASA Astrophysics Data System (ADS)

2006-08-01

produce gamma rays and X-rays have disks of material rotating rapidly about the central object," Soderberg said. The powerful gamma ray bursts tap the tremendous gravitational energy of their black hole to produce strong beams of energetic radiation, while less-energetic X-ray bursts like the Feburary event tap energy from the strong magnetic field of the magnetar, the scientists speculated. "This discovery means that the 'zoo' of cosmic explosions has just gotten more numerous and more diverse. It also means that our understanding of how the cores of massive stars collapse to produce this variety of explosions is less complete than we had thought," Frail added. Multiwavelength follow-up observations were required by the team to measure the total energy release of the explosion. In particular, Soderberg adds that "Radio observations with the Very Large Array were additionally required to determine the geometry of the ejecta. We find that unlike typical GRBs which produce pencil-beam jets, this object more resembles a spherical explosion." In addition to Soderberg and Frail, the research team includes Shri Kulkarni. Ehud Nakar, Edo Berger, Brian Cameron, Avishay Gal-Yam, Re'em Sari, Mansi Kasiwal, Eran Ofek, Arne Rau, Brad Cenko, Eric Persson and Dae-Sik Moon of Caltech, Derrick Fox and Dave Burrows of Pennsylvania State University, Roger Chevalier of the University of Virginia, Tsvi Piran of the Hebrew University, Paul Price of the University of Hawaii, Brian Schmidt of Mount Stromlo Observatory in Australia, Guy Pooley of the Mullard Radio Astronomy Observatory in the UK, Bryan Penprase of Pomona College, and Neil Gehrels of the NASA Goddard Space Flight Center. The National Radio Astronomy Observatory is a facility of the National Science Foundation, operated under cooperative agreement by Associated Universities, Inc. http://www.nrao.edu/

Cosmic Blasts Much More Common, Astronomers Discover

NASA Astrophysics Data System (ADS)

2006-08-01

explosions is that the blasts that produce gamma rays and X-rays have disks of material rotating rapidly about the central object," Soderberg said. The powerful gamma ray bursts tap the tremendous gravitational energy of their black hole to produce strong beams of energetic radiation, while less-energetic X-ray bursts like the Feburary event tap energy from the strong magnetic field of the magnetar, the scientists speculated. "This discovery means that the 'zoo' of cosmic explosions has just gotten more numerous and more diverse. It also means that our understanding of how the cores of massive stars collapse to produce this variety of explosions is less complete than we had thought," Frail added. Multiwavelength follow-up observations were required by the team to measure the total energy release of the explosion. In particular, Soderberg adds that "Radio observations with the Very Large Array were additionally required to determine the geometry of the ejecta. We find that unlike typical GRBs which produce pencil-beam jets, this object more resembles a spherical explosion." In addition to Soderberg and Frail, the research team includes Shri Kulkarni. Ehud Nakar, Edo Berger, Brian Cameron, Avishay Gal-Yam, Re'em Sari, Mansi Kasiwal, Eran Ofek, Arne Rau, Brad Cenko, Eric Persson and Dae-Sik Moon of Caltech, Derrick Fox and Dave Burrows of Pennsylvania State University, Roger Chevalier of the University of Virginia, Tsvi Piran of the Hebrew University, Paul Price of the University of Hawaii, Brian Schmidt of Mount Stromlo Observatory in Australia, Guy Pooley of the Mullard Radio Astronomy Observatory in the UK, Bryan Penprase of Pomona College, and Neil Gehrels of the NASA Goddard Space Flight Center. The National Radio Astronomy Observatory is a facility of the National Science Foundation, operated under cooperative agreement by Associated Universities, Inc.

[Tuberculin reactivity among ninth-grade schoolchildren in the city of Havana, Cuba].

PubMed

Borroto Gutiérrez, Susana M; González Ochoa, Edilberto; Armas Pérez, Luisa; Urbino López-Chávez, Amelia; Martínez, Ana Maribel; Llanes, María Josefa; Sevy Court, José; Carreras Corzo, Libertad

2003-09-01

infección tuberculosa en escolares de 14 años de Ciudad de La Habana, Cuba. Métodos. Mediante un muestreo monoetápico por conglomerados se aplicaron 1 936 pruebas de Mantoux (tuberculina) a estudiantes de noveno grado del curso escolar 1999-2000 (cohorte de los nacidos en 1985) de 20 escuelas secundarias básicas seleccionadas aleatoriamente en Ciudad de La Habana, Cuba. Las pruebas se realizaron según la técnica estándar recomendada por la Organización Mundial de la Salud y se evaluaron a las 72 horas. Se calcularon los porcentajes de reactividad y el diámetro medio de las induraciones cutáneas en la cohorte y según el sexo. Las comparaciones de medias y porcentajes se realizaron mediante la prueba de ji al cuadrado con 95% de confiabilidad. Se usó el programa Epi Info v. 6.0. Resultados. Noventa y seis por ciento de las pruebas leídas fueron negativas (0-4 mm), 2,5% fueron dudosas (5-9 mm) y 1,5% resultaron positivas ( >/= 10 mm). El porcentaje de reactividad disminuyó a 0,1% cuando se utilizó un valor de corte de 15 mm. El diámetro medio de las induraciones fue de 0,41 mm y no se detectó ninguna diferencia estadísticamente significativa en función del sexo. Conclusiones. La proporción de personas que reaccionaron a la tuberculina, usando como punto de corte un diámetro de induración cutánea de 10 mm, fue muy baja (1,5%) en este estudio y mucho más baja (0,1%) cuando se utilizó 15 mm como punto de corte. Si se toma en cuenta la baja frecuencia de tuberculosis bacilífera en Cuba y que hay una relación inversa entre el tiempo transcurrido desde la aplicación de la vacuna a base del bacilo de Calmette-Guérin (BCG) y la intensidad de la respuesta a la tuberculina, las reacciones cutáneas con un diámetro de induración >/= 10 mm podrían ser la expresión de una infección natural, por lo que, en ese caso, la prevalencia puntual de infección tuberculosa en este grupo de escolares sería de 1,5%.

Library of Congress Model, Perspective View

NASA Technical Reports Server (NTRS)

2004-01-01

The Shuttle Radar Topography Mission (SRTM) has produced the first high-resolution, near-global elevation dataset of Earth. In recognition of this achievement, and as an illustration of the data, the United States Library of Congress now displays a 'solid terrain model' of Los Angeles and adjacent mountainous terrain. The model was created by carving a high-density foam block using computer-guided drills that referenced the SRTM dataset. The block was then covered with a Landsat satellite image using computer-guided paint guns that referenced both the Landsat image and the SRTM dataset. The view shown here mimics the actual model on display at the Library of Congress and was generated from the same satellite image and elevation data sets.

The model shows the Pacific Ocean and Santa Monica Mountains along the Malibu Coast (lower left), San Fernando Valley (left center), downtown Los Angeles (bottom center), San Gabriel and Pomona Valleys (lower right), San Gabriel Mountains (right center to far right), and part of the Mojave Desert (upper right). Colors are enhanced true color with added topographic shading, and elevation differences are exaggerated 1.5 times. The view is toward the north-northwest.

The Los Angeles region was chosen for the Library of Congress model because it illustrates so many ways that topography affects the daily lives of people. The region consists of a coastal plain, inland valleys, mountains up to 3068 meters (10,064 feet), and a desert interior. Topography blocks the landward influence of marine airmasses here such that summer temperatures often differ by 40 degrees Fahrenheit (22 C) across this region at a given moment even at similar elevations. Temperatures also typically cool with rising elevation, and winter storms drop most of their moisture in the mountains, leaving little rainfall for areas further inland, thus creating the deserts.

Topography also controls the land use pattern. The mountains are mostly very

Library of Congress Model, Anaglyph

NASA Technical Reports Server (NTRS)

2004-01-01

The Shuttle Radar Topography Mission (SRTM) has produced the first high-resolution, near-global elevation dataset of Earth. In recognition of this achievement, and as an illustration of the data, the United States Library of Congress now displays a 'solid terrain model' of Los Angeles and adjacent mountainous terrain. The model was created by carving a high-density foam block using computer-guided drills that referenced the SRTM dataset. The block was then covered with a Landsat satellite image using computer-guided paint guns that referenced both the Landsat image and the SRTM dataset. The view shown here mimics the actual model on display at the Library of Congress and was generated from the same satellite image and elevation data sets.

Anaglyph glasses are required to see this view in three-dimensions. Anaglyph glasses cover the left eye with a red filter and cover the right eye with a blue filter.

The model shows the Pacific Ocean and Santa Monica Mountains along the Malibu Coast (lower left), San Fernando Valley (left center), downtown Los Angeles (bottom center), San Gabriel and Pomona Valleys (lower right), San Gabriel Mountains (right center to far right), and part of the Mojave Desert (upper right). Colors are enhanced true color with added topographic shading, and elevation differences are exaggerated 1.5 times. The view is toward the north-northwest.

The Los Angeles region was chosen for the Library of Congress model because it illustrates so many ways that topography affects the daily lives of people. The region consists of a coastal plain, inland valleys, mountains up to 3068 meters (10,064 feet), and a desert interior. Topography blocks the landward influence of marine airmasses here such that summer temperatures often differ by 40 degrees Fahrenheit (22 C) across this region at a given moment even at similar elevations. Temperatures also typically cool with rising elevation, and winter storms drop most of their moisture in the

Evaluation of long-term water-level declines in basalt aquifers near Mosier, Oregon

USGS Publications Warehouse

Burns, Erick R.; Morgan, David S.; Lee, Karl K.; Haynes, Jonathan V.; Conlon, Terrence D.

2012-01-01

The Mosier area lies along the Columbia River in northwestern Wasco County between the cities of Hood River and The Dalles, Oregon. Major water uses in the area are irrigation, municipal supply for the city of Mosier, and domestic supply for rural residents. The primary source of water is groundwater from the Columbia River Basalt Group (CRBG) aquifers that underlie the area. Concerns regarding this supply of water arose in the mid-1970s, when groundwater levels in the orchard tract area began to steadily decline. In the 1980s, the Oregon Water Resources Department (OWRD) conducted a study of the aquifer system, which resulted in delineation of an administrative area where parts of the Pomona and Priest Rapids aquifers were withdrawn from further appropriations for any use other than domestic supply. Despite this action, water levels continued to drop at approximately the same, nearly constant annual rate of about 4 feet per year, resulting in a current total decline of between 150 and 200 feet in many wells with continued downward trends. In 2005, the Mosier Watershed Council and the Wasco Soil and Water Conservation District began a cooperative investigation of the groundwater system with the U.S. Geological Survey. The objectives of the study were to advance the scientific understanding of the hydrology of the basin, to assess the sustainability of the water supply, to evaluate the causes of persistent groundwater-level declines, and to evaluate potential management strategies. An additional U.S. Geological Survey objective was to advance the understanding of CRBG aquifers, which are the primary source of water across a large part of Oregon, Washington, and Idaho. In many areas, significant groundwater level declines have resulted as these aquifers were heavily developed for agricultural, municipal, and domestic water supplies. Three major factors were identified as possible contributors to the water-level declines in the study area: (1) pumping at rates that

Obituary: David Fulmer Bender, 1913-2004

NASA Astrophysics Data System (ADS)

Miller, Sylvia L.

2004-12-01

David Fulmer Bender died in San Diego, California, on 13 September 2004, at the age of 91. His heart stopped suddenly while he was dancing. His pioneering work in establishing comprehensive, computer-accessible ephemerides of asteroids and comets found many applications, including the first-ever visit to an asteroid, Gaspra, by an interplanetary spacecraft. Dave was born in Reno, Nevada, on 10 February 1913, to Homer Charles Bender and Susan Bowers Bender. The family moved to Spokane, Washington, while Dave was very young. His father was a civil engineer and a graduate of MIT, who helped design bridges and dams throughout the Northwest, including the Grand Coolie Dam. Dave had a brother, Phillip (now deceased), who was one year younger. Advancing rapidly in the Spokane school system, Dave finished high school when he was 15 years old. At 16 he moved to Pasadena, California, and began his studies at the California Institute of Technology (Caltech). In addition to pursuing his course work, he was active in track and football, a tendency toward physical exercise that stayed with him for the rest of his life. It was probably during these years that Dave heard a lecture by Albert Einstein, as mentioned to colleagues many years later. Dave received a BS degree in physics in 1933, an MS in 1934, and a PhD in 1937, all from Caltech. His dissertation was entitled, "The Index of Refraction of Air in the Photographic Infrared." During his sophomore year he found his way to Pomona College in Claremont, California, where he met his future wife, Elizabeth Boyden at a social gathering. They were married in 1935. Dave's academic career spanned the years from 1937 to 1970, initially at Louisiana State University, Vanderbilt University, and then Fisk. As a life-long pacifist and conscientious objector, Dave served alternate duty during World War II. In 1946 he joined the faculty of the physics department at Whittier College in California, where he became the department chair and