Host status of grapefruit and Valencia oranges for Anastrepha serpentina and Anastrepha ludens (Diptera: Tephritidae).

PubMed

Mangan, Robert L; Thomas, Donald B; Moreno, Aleena M Tarshis

2011-04-01

Anastrepha serpentina (Wiedemann) (Diptera: Tephritidae) is sporadically captured in the Rio Grande Valley of Texas. Although its preferred hosts are in the Sapotaceae family, several varieties of Citrus, including grapefruit and oranges are listed as alternate hosts. Although Mexican fruit fly, Anastrepha ludens (Loew), is known to be a major pest of Citrus, doubt exists as to the status of Citrus as a breeding host for A. serpentina. To evaluate the host status of commercial Citrus for A. serpentina we compared oviposition and development with that of A. ludens under laboratory conditions with 'Rio Red' grapefruit (Citrus paradisi MacFayden) and 'Valencia' oranges [Citrus sinensis (L.) Osbeck] in different stages of maturity. Both fly species oviposited in early season fruit in which the eggs and larvae died in the fruit albedo. Survival of either species to the adult stage occurred in later season grapefruit. In oranges, no A. serpentina larvae survived compared with 150 A. ludens surviving to adults. Survival on both Citrus species was much lower for A. serpentina, only approximately 5% of eggs eclosed into larvae in grapefruit compared with approximatley 50% for A. ludens. In oranges approximately 16% of A. serpentina eggs eclosed compared with approximately 76% for A. ludens. In grapefruit, only one fourth as many A. serpentina larvae survived to the adult stage compared with A. ludens. Additional experiments were performed in a greenhouse on small, caged trees of la coma (Sideroxylon celastrinum H.B.K.), a Texas species of Sapotaceae. The A. serpentina females readily oviposited into these berries and normal adults emerged. The present low incidence of the adults, coupled with the high mortality during development of the larvae, suggests that Texas citrus is unlikely to support a breeding population of A. serpentina.

Changes in Volatile and Non-Volatile Flavor Chemicals of "Valencia" Orange Juice over the Harvest Seasons.

PubMed

Bai, Jinhe; Baldwin, Elizabeth A; McCollum, Greg; Plotto, Anne; Manthey, John A; Widmer, Wilbur W; Luzio, Gary; Cameron, Randall

2016-01-04

Florida "Valencia" oranges have a wide harvest window, covering four months after first reaching the commercial maturity. However, the influence of harvest time on juice flavor chemicals is not well documented, with the exception of sugars and acids. Therefore, we investigated the major flavor chemicals, volatile (aroma), non-volatile (taste) and mouth feel attributes, in the two harvest seasons (March to June in 2007 and February to May in 2012). Bitter limonoid compounds, limonin and nomilin, decreased gradually. Out of a total of 94 volatiles, 32 increased, 47 peaked mid to late season, and 15 decreased. Juice insoluble solids and pectin content increased over the season; however, pectin methylesterase activity remained unchanged. Fruit harvested in the earlier months had lower flavor quality. Juice from later harvests had a higher sugar/acid ratio with less bitterness, while, many important aroma compounds occurred at the highest concentrations in the middle to late season, but occurred at lower concentrations at the end of the season. The results provide information to the orange juice processing industry for selection of optimal harvest time and for setting of precise blending strategy.

Effects of open-top chambers on 'Valencia' orange trees

DOE Office of Scientific and Technical Information (OSTI.GOV)

Olszyk, D.M.; Takemoto, B.K.; Kats, G.

1992-01-01

Young 'Valencia' orange trees (Citrus sinensis(L) Osbeck) were grown for four years in large open-top chambers with ambient (nonfiltered) air or in outside air to determine any effects of the chambers on the air pollutant susceptibility of the trees. Long-term ozone average concentrations (12 hours, growing season) were 8% lower, and cumulative ozone dose (hourly values >0.1 microL/L) was 29% lower in ambient chambers compared to outside air. Fruit yields were much higher (>39%) for ambient chamber trees than for outside trees over three harvests, due at least partly to less fruit drop during the growing season for ambient chambermore » trees. Ambient chamber trees were much larger than outside trees and produced over twice as much leaf material over four years of study. Leaves on ambient chamber trees were larger and less dense than on outside trees. Leaves on ambient chamber trees were under more stress than leaves on outside trees during summer months; with lower stomatal conductances (14% average) and transpiration rates (12%), and more negative leaf water pressure potentials (28%). In contrast, leaves on ambient chamber trees had higher net photosynthetic rates (13%) and higher leaf starch concentrations prior to tree flowering (31%), than leaves on outside trees. While these results indicated large long-term impacts on tree growth which must be considered when using open-top chambers, they did not indicate any net effect of chambers on the air pollutant susceptibility of trees which would limit the usefulness of chamber tree data for air quality impact assessment purposes.« less

The effect of citrus-derived oil on bovine blood neutrophil response in vitro

USDA-ARS?s Scientific Manuscript database

Research on the use of products with germicidal activity to treat or prevent microbial invasion as alternatives to antibiotic drug use is expanding. To our knowledge, potential for cold-pressed terpeneless Valencia orange oil (TCO) to control mastitis in dairy cows as an alternative therapy has not...

Deficit irrigation and maturation stage influence quality and flavonoid composition of 'Valencia' orange fruit.

PubMed

Grilo, Filipa S; Di Stefano, Vita; Lo Bianco, Riccardo

2017-04-01

Effects of continuous deficit irrigation (DI) and partial rootzone drying (PRD) treatments (50% ETc) in comparison with full irrigation (CI, 100% ETc) were investigated during 'Valencia' orange fruit maturation. Ultra-high-performance liquid chromatography/high-resolution mass spectrometry was used to quantify hesperidin, narirutin, tangeritin, nobiletin, didymin and neoeriocitrin in the fruit juice and peel. No significant effect of irrigation was found on yield, juice soluble solids or acidity. Juice color was not influenced by irrigation or harvest date, whereas peel color increased during maturation and was more pronounced in CI and PRD fruits. Juice acidity reached a peak in May, while soluble solids increased linearly throughout maturation. Hesperidin was the major flavanone detected during maturation, with concentrations 200-fold higher in the fruit peel than in the juice. In the peel, narirutin, didymin and neoeriocitrin decreased while hesperidin, nobiletin and tangeritin increased with maturation. Narirutin synthesis in the orange fruit was insensitive to irrigation strategy. In fruit peels, PRD and DI induced the decline of hesperidin, nobiletin and tangeritin only in June, whereas in the juice, deficit irrigation treatments induced an increase in hesperidin and didymin. These results suggest that deficit irrigation, in particular the conditions imposed with PRD, may cause a significant accumulation shift of total flavonoids from the fruit peel into the juice, with a positive impact on juice quality and nutritional value. Fruit compositional changes during maturation also suggest that late harvest can improve fruit palatability and nutritional quality under the cultural and environmental conditions of this study. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

Isolation and biological activities of decanal, linalool, valencene, and octanal from sweet orange oil.

PubMed

Liu, Kehai; Chen, Qiulin; Liu, Yanjun; Zhou, Xiaoyan; Wang, Xichang

2012-11-01

Product 1 (82.25% valencene), product 2 (73.36% decanal), product 3 (78.12% octanal), and product 4 (90.61% linalool) were isolated from sweet orange oil by combined usage of molecular distillation and column chromatography. The antioxidant activity of sweet orange oil and these products was investigated using 2,2-diphenyl-1-picrylhydrazyl and reducing power assays. In this test, product 1 (82.25% valencene), product 2 (73.36% decanal), and product 4 (90.61% linalool) had antioxidant activity, but lower than sweet orange oil. The antimicrobial activity was investigated in order to evaluate their efficacy against 5 microorganisms. The results showed that sweet orange oil, product 2 (73.36% decanal), product 3 (78.12% octanal), and product 4 (90.61% linalool) had inhibitory and bactericidal effect on the test microorganisms (except Penicillium citrinum). Valencene did not show any inhibitory effect. Saccharomyces cerivisiae was more susceptible, especially to the crude sweet orange oil (minimal inhibitory concentration 6.25 μL/mL). The cytotoxicity was evaluated on Hela cells using the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. All test samples showed significant cytotoxicity on the cell lines with IC(50) values much less than 20 μg/mL. © 2012 Institute of Food Technologists®

The effect of chloroform, orange oil and eucalyptol on root canal transportation in endodontic retreatment.

PubMed

Karataş, Ertuğrul; Kol, Elif; Bayrakdar, İbrahim Şevki; Arslan, Hakan

2016-04-01

The purpose of the present study was to assess the effect of solvents on root canal transportation in endodontic retreatment. Sixty extracted human permanent mandibular first molars with curved root canals were selected. All of the root canals were prepared using Twisted File Adaptive instruments (SybronEndo, Orange, CA, USA) and filled with gutta-percha and AH Plus sealer (Dentsply DeTrey, Konstanz, Germany) using the cold lateral compaction technique. The teeth were assigned to four retreatment groups as follows (n = 15): eucalyptol, chloroform, orange oil and control. The canals were scanned using cone-beam computed tomography scanning before and after instrumentation. The chloroform group showed a significantly higher mean transportation value than the orange oil and control groups at the 3 and 5 mm levels (P = 0.011 and P = 0.003, respectively). There was no significant difference among the orange oil, eucalyptol and control groups in terms of canal transportation (P > 0.61). The chloroform led to more canal transportation than the eucalyptol and orange oil during endodontic retreatment. © 2015 Australian Society of Endodontology.

Fumigant Activity of Sweet Orange Essential Oil Fractions Against Red Imported Fire Ants (Hymenoptera: Formicidae).

PubMed

Hu, Wei; Zhang, Ning; Chen, Hongli; Zhong, Balian; Yang, Aixue; Kuang, Fan; Ouyang, Zhigang; Chun, Jiong

2017-08-01

Sweet orange oil fractions were prepared by molecular distillation of cold-pressed orange oil from sample A (Citrus sinensis (L.) 'Hamlin' from America) and sample B (Citrus sinensis Osbeck 'Newhall' from China) respectively, and their fumigant activities against medium workers of red imported fire ants (Solenopsis invicta Buren) were investigated. The volatile composition of the orange oil fractions was identified and quantified using GC-MS. Fractions from sample A (A1, A2, and A3) contained 23, 37, and 48 chemical constituents, and fractions from sample B (B1, B2, and B3) contained 18, 29, and 26 chemical constituents, respectively. Monoterpenes were the most abundant components, accounting for 73.56% to 94.86% of total orange oil fractions, among which D-limonene (65.28-80.18%), β-pinene (1.71-5.58%), 3-carene (0.41-4.01%), β-phellandrene (0.58-2.10%), and linalool (0.31-2.20%) were major constituents. Fumigant bioassay indicated that all orange oil fractions exerted good fumigant toxicity against workers of fire ants at 3, 5, 10, and 20 mg/centrifuge tubes, and B1 had the strongest insecticidal potential, followed by A1, B2, A2, B3, and A3. The fractions composed of more high volatile molecules (A1 and B1) showed greater fumigant effects than others. Compounds linalool and D-limonene, which were the constituents of the orange oil, exhibited excellent fumigant toxicity against red imported fire ant workers. Linalool killed red imported fire ant workers completely at 5, 10, and 20 mg/tube after 8 h of treatment, and D-limonene induced >86% mortality at 8 h of exposure. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Extraction of essential oil from baby Java orange (Citrus sinensis) solid waste using water and steam distillation

NASA Astrophysics Data System (ADS)

Dewi, I. A.; Prastyo, A. M.; Wijana, S.

2018-03-01

Baby java orange (Citrus sinensis) is commonly consumed as juice. Processing of baby java orange leaves organic waste which consist of the mesocarp, exocarp, seed, and wall of the orange. Therefore, it is necessary to process baby java orange waste to be valuable products. The purpose of this study was to provide added value to unutilized baby java orange waste, and to find out the pretreatment of time-delay process that maximize the yield of essential oil produced. Essential oil processing can be done by water and steam distillation. The study used randomized block design with one factor namely distillation time-delay process by air drying consisted of 4 levels i.e. the distillation delay for 2, 4, 6, and 8 days. The best treatment was determined based on the yield. The best essential oil from baby java orange waste was obtained from the treatment of distillation delay-process of 8 days. This pretreatment generated yield value of 0.63% with moisture content of 24.21%. By estimating the price of essential oil showed that this effort not only reduced the bulky organic waste but also provided potential economical value.

Timing of the inhibitory effect of fruit on return bloom of 'Valencia' sweet orange (Citrus sinensis (L.) Osbeck).

PubMed

Martínez-Fuentes, Amparo; Mesejo, Carlos; Reig, Carmina; Agustí, Manuel

2010-08-30

In Citrus the inhibitory effect of fruit on flower formation is the main cause of alternate bearing. Although there are some studies reporting the effect on flowering of the time of fruit removal in a well-defined stage of fruit development, few have investigated the effect throughout the entire fruit growth stage from early fruitlet growth to fruit maturity. The objective of this study was to determine the phenological fruit developmental stage at which the fruit begins its inhibitory effect on flowering in sweet orange by manual removal of fruits, and the role of carbohydrates and nitrogen in the process. Fruit exerted its inhibitory effect from the time it was close to reaching its maximum weight, namely 90% of its final size (November) in the present experiments, to bud sprouting (April). The reduction in flowering paralleled the reduction in bud sprouting. This reduction was due to a decrease in the number of generative sprouted buds, whereas mixed-typed shoots were largely independent of the time of fruit removal, and vegetative shoots increased in frequency. The number of leaves and/or flowers per sprouted shoot was not significantly modified by fruit load. In 'Valencia' sweet orange, fruit inhibits flowering from the time it completes its growth. Neither soluble sugar content nor starch accumulation in leaves due to fruit removal was related to flowering intensity, but some kind of imbalance in nitrogen metabolism was observed in trees tending to flower scarcely. Copyright (c) 2010 Society of Chemical Industry.

Cold plasma treatment of valencia oranges reduces persistence of salmonella

USDA-ARS?s Scientific Manuscript database

Introduction: Fresh fruits such as oranges are an increasingly popular food, consumed directly or as ingredients in “raw” juice blends prepared at home or commercially at the point of consumption. However, because of past outbreaks associated with orange juice, and the risk of cross-contamination du...

Comparative evaluation of the effect of sweet orange oil-diesel blend on performance and emissions of a multi-cylinder compression ignition engine

NASA Astrophysics Data System (ADS)

Rahman, S. M. Ashrafur; Hossain, F. M.; Van, Thuy Chu; Dowell, Ashley; Islam, M. A.; Rainey, Thomas J.; Ristovski, Zoran D.; Brown, Richard J.

2017-06-01

In 2014, global demand for essential oils was 165 kt and it is expected to grow 8.5% per annum up to 2022. Every year Australia produces approximately 1.5k tonnes of essential oils such as tea tree, orange, lavender, eucalyptus oil, etc. Usually essential oils come from non-fatty areas of plants such as the bark, roots, heartwood, leaves and the aromatic portions (flowers, fruits) of the plant. For example, orange oil is derived from orange peel using various extraction methods. Having similar properties to diesel, essential oils have become promising alternate fuels for diesel engines. The present study explores the opportunity of using sweet orange oil in a compression ignition engine. Blends of sweet orange oil-diesel (10% sweet orange oil, 90% diesel) along with neat diesel fuel were used to operate a six-cylinder diesel engine (5.9 litres, common rail, Euro-III, compression ratio 17.3:1). Some key fuel properties such as: viscosity, density, heating value, and surface tension are presented. Engine performance (brake specific fuel consumption) and emission parameters (CO, NOX, and Particulate Matter) were measured to evaluate running with the blends. The engine was operated at 1500 rpm (maximum torque condition) with different loads. The results from the property analysis showed that sweet orange oil-diesel blend exhibits lower density, viscosity and surface tension and slightly higher calorific value compared to neat diesel fuel. Also, from the engine test, the sweet orange oil-diesel blend exhibited slightly higher brake specific fuel consumption, particulate mass and particulate number; however, the blend reduced the brake specific CO emission slightly and brake specific NOX emission significantly compared to that of neat diesel.

Commercial Origanum compactum Benth. and Cinnamomum zeylanicum Blume essential oils against natural mycoflora in Valencia rice.

PubMed

Santamarina, M Pilar; Roselló, Josefa; Sempere, Francisca; Giménez, Silvia; Blázquez, M Amparo

2015-01-01

Chemical composition of commercial Origanum compactum and Cinnamomum zeylanicum essential oils and the antifungal activity against pathogenic fungi isolated from Mediterranean rice grains have been investigated. Sixty-one compounds accounting for more than 99.5% of the total essential oil were identified by using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Carvacrol (43.26%), thymol (21.64%) and their biogenetic precursors p-cymene (13.95%) and γ-terpinene (11.28%) were the main compounds in oregano essential oil, while the phenylpropanoids, eugenol (62.75%), eugenol acetate (16.36%) and (E)-cinnamyl acetate (6.65%) were found in cinnamon essential oil. Both essential oils at 300 μg/mL showed antifungal activity against all tested strains. O. compactum essential oil showed the best antifungal activity towards Fusarium species and Bipolaris oryzae with a total inhibition of the mycelial growth. In inoculated rice grains at lower doses (100 and 200 μg/mL) significantly reduced the fungal infection, so O. compactum essential oil could be used as ecofriendly preservative for field and stored Valencia rice.

Formulating orange oil-in-water beverage emulsions for effective delivery of bioactives: Improvements in chemical stability, antioxidant activity and gastrointestinal fate of lycopene using carrier oils.

PubMed

Meroni, Erika; Raikos, Vassilios

2018-04-01

The influence of carrier oil type on the chemical stability, antioxidant properties and bioaccessibility of lycopene in orange oil-in-water beverage emulsions was investigated. The emulsions were formulated with orange oil (A), which was partially (50%) replaced with tributyrin (B) or corn oil (C) because of their distinctively different fatty acid composition. The addition of corn oil enhanced the physical stability of the beverage during chilled storage by inhibiting Ostwald ripening. The formation of oxidation products was insignificant after storage for 28 days at 4 °C, regardless the type of added oil. Lycopene was more susceptible to chemical degradation in the presence of unsaturated, long chain triglycerides and the retention followed the order: A (87.94%), B (64.41%) and C (57.39%). Interestingly, bioaccessibility of lycopene was significantly lower for emulsions formulated with 50% corn oil as opposed to 100% orange oil as indicated by the simulated in vitro gastric digestion model. Copyright © 2018 Elsevier Ltd. All rights reserved.

Biodiesel from Citrus reticulata (Mandarin orange) seed oil, a potential non-food feedstock

USDA-ARS?s Scientific Manuscript database

Oil extracted from Citrus reticulata (Mandarin orange) seeds was investigated as a potential feedstock for the production of biodiesel. The biodiesel fuel was prepared by sodium methoxide-catalyzed transesterification of the oil with methanol. Fuel properties that were determined include cetane numb...

Characterization of Coconut cadang-cadang viroid variants from oil palm affected by orange spotting disease in Malaysia.

PubMed

Wu, Y H; Cheong, L C; Meon, S; Lau, W H; Kong, L L; Joseph, H; Vadamalai, G

2013-06-01

A 246-nt variant of Coconut cadang-cadang viroid (CCCVd) has been identified and described from oil palms with orange spotting symptoms in Malaysia. Compared with the 246-nt form of CCCVd from coconut, the oil palm variant substituted C(31)→U in the pathogenicity domain and G(70)→C in the central conserved domain. This is the first sequence reported for a 246-nt variant of CCCVd in oil palms expressing orange spotting symptoms.

GC-MS analysis of headspace and liquid extracts for metabolomic differentiation of citrus Huanglongbing and zinc deficiency in leaves of 'Valencia' sweet orange from commercial groves.

PubMed

Cevallos-Cevallos, Juan Manuel; García-Torres, Rosalía; Etxeberria, Edgardo; Reyes-De-Corcuera, José Ignacio

2011-01-01

Citrus Huanglongbing (HLB) is considered the most destructive citrus disease worldwide. Symptoms-based detection of HLB is difficult due to similarities with zinc deficiency. To find metabolic differences between leaves from HLB-infected, zinc-deficient, and healthy 'Valencia' orange trees by using GC-MS based metabolomics. Analysis based on GC-MS methods for untargeted metabolite analysis of citrus leaves was developed and optimized. Sample extracts from healthy, zinc deficient, or HLB-infected sweet orange leaves were submitted to headspace solid phase micro-extraction (SPME) and derivatization treatments prior to GC-MS analysis. Principal components analysis achieved correct classification of all the derivatized liquid extracts. Analysis of variance revealed 6 possible biomarkers for HLB, of which 5 were identified as proline, β-elemene, (-)trans- caryophyllene, and α-humulene. Significant (P < 0.05) differences in oxo-butanedioic acid, arabitol, and neo-inositol were exclusively detected in samples from plants with zinc deficiency. Levels of isocaryophyllen, α-selinene, β-selinene, and fructose were significantly (P < 0.05) different in healthy leaves only. Results suggest the potential of using identified HLB biomarkers for rapid differentiation of HLB from zinc deficiency. Copyright © 2010 John Wiley & Sons, Ltd.

An attempt of postharvest orange fruit rot control using essential oils from Mediterranean plants.

PubMed

Camele, Ippolito; De Feo, Vincenzo; Altieri, Luciana; Mancini, Emilia; De Martino, Laura; Luigi Rana, Gian

2010-12-01

Twelve essential oils from Mediterranean aromatic plants were tested at different doses against four fungi known as causal agents of post-harvest orange fruit rot: Botrytis cinerea, Penicillium italicum, Phytophthora citrophthora, and Rhizopus stolonifer. Essential oils were obtained from Hyssopus officinalis, Lavandula angustifolia, Majorana hortensis, Melissa officinalis, Ocimum basilicum, Origanum vulgare, Salvia officinalis, and Thymus vulgaris (Family Lamiaceae), Verbena officinalis (Family Verbenaceae), and Pimpinella anisum, Foeniculum vulgare, and Carum carvi (Family Apiaceae). Because preliminary in vitro experiments showed that only the oils from V. officinalis, T. vulgaris, and O. vulgare exhibited some fungistatic activity against the above-named fungi, these three essential oils were used in successive in vivo tests carried out to protect healthy "Washington navel" orange fruits from artificial infection by the same micromycetes. The essential oil of T. vulgaris, at a 2,000 ppm dose, controlled fruit rot by B. cinerea, P. citrophthora, and R. stolonifer but was ineffective against P. italicum. Essential oils of V. officinalis and O. vulgare inhibited infection by the first two fungi and only by P. citrophthora, respectively. This finding represents an important result, with the goal of using the essential oils as natural preservatives for food products, due to their positive effect on their safety and shelf life.

Differentiation between Flavors of Sweet Orange (Citrus sinensis) and Mandarin (Citrus reticulata).

PubMed

Feng, Shi; Suh, Joon Hyuk; Gmitter, Frederick G; Wang, Yu

2018-01-10

Pioneering investigations referring to citrus flavor have been intensively conducted. However, the characteristic flavor difference between sweet orange and mandarin has not been defined. In this study, sensory analysis illustrated the crucial role of aroma in the differentiation between orange flavor and mandarin flavor. To study aroma, Valencia orange and LB8-9 mandarin were used. Their most aroma-active compounds were preliminarily identified by aroma extract dilution analysis (AEDA). Quantitation of key volatiles followed by calculation of odor activity values (OAVs) further detected potent components (OAV ≥ 1) impacting the overall aromatic profile of orange/mandarin. Follow-up aroma profile analysis revealed that ethyl butanoate, ethyl 2-methylbutanoate, octanal, decanal, and acetaldehyde were essential for orange-like aroma, whereas linalool, octanal, α-pinene, limonene, and (E,E)-2,4-decadienal were considered key components for mandarin-like aroma. Furthermore, an unreleased mandarin hybrid producing fruit with orange-like flavor was used to validate the identification of characteristic volatiles in orange-like aroma.

Allergenicity of orange juice and orange seeds: a clinical study.

PubMed

Zhu, S L; Ye, S T; Yu, Y

1989-06-01

Oranges are considered to be common allergenic fruits in China. They may induce severe food allergy in sensitive individuals. Allergic histories were analyzed in 26 orange-sensitive patients. Intradermal tests with extracts of orange juice and seeds were performed in 16 out of the 26 patients. P-K test was performed in one patient. The allergic history analysis suggested that clinical symptoms of some orange-allergic subjects were different from other fruit allergies but similar to nut and other oil plant seed allergies. The skin test and P-K test showed that the major allergenic components of orange reside in orange seeds instead of orange juice. Systemic reactions developed in 5 patients after intradermal tests with 1:20-200 (w/v) orange seed extracts. We considered that orange seed contains high potent allergens which may induce orange sensitivity due to careless chewing of orange seeds.

Preparation of fatty acid methyl esters from Osage orange (Maclura pomifera) oil and evaluation as biodiesel

USDA-ARS?s Scientific Manuscript database

Fatty acid methyl esters were prepared in high yield by transesterification of Osage orange (Maclura pomifera) oil. Extracted using supercritical CO2, the crude oil was initially treated with mineral acid and methanol to lower its content of free fatty acids, thus rendering it amenable to homogeneou...

Reduction of salmonella on valencia oranges by cold plasma treatment

USDA-ARS?s Scientific Manuscript database

Orange juice has been the source of recurrent food borne illness outbreaks, primarily associated with Salmonella. There is a need for antimicrobial interventions which can effectively eliminate pathogens from fruit surfaces and reduce the risk of cross-contamination during peeling and processing. To...

Effect of Liberibacter infection (huanglongbing or "greening" disease) of citrus on orange juice flavor quality by sensory evaluation.

PubMed

Plotto, Anne; Baldwin, Elizabeth; McCollum, Greg; Manthey, John; Narciso, Jan; Irey, Mike

2010-05-01

Some anecdotal reports suggest that infection of citrus trees with Candidatus Liberibacter asiaticus (Las), the suspected causal agent of huanglongbing (HLB) disease, imparts off flavor to orange juice. It is of interest to the industry to know how Las infection affects juice quality with respect to cultivar, maturity, or processing method. Hamlin, Midsweet, and Valencia oranges were harvested over 2 y from trees that tested negative (Las-) or positive (Las+) for Las from different groves and included normal looking (nonsymptomatic) and symptomatic fruit (small, green, and lopsided) from Las+ trees. In the 1st year, fruit were manually juiced, while in the 2nd year, a commercial process was used. Juice from Las+ trees was compared to juice from Las- trees in difference-from-control tests, and by descriptive analysis. Results showed large variability due to tree, harvest date, and cultivar. Juice from Hamlin Las+ trees tended to be more bitter and sour than its Las- counterpart. In contrast, hand processed Valencia juice from Las+ trees was perceived to have some off-flavor and bitterness compared to control, but the following year, commercially processed Valencia juice from Las+ trees was perceived to be only slightly more sour than juice from Las- trees for the April harvest, and to be sweeter for the June harvest. When juice from individual replicates was pooled to be more representative of a commercial situation, there was no difference between Las+ and Las- juice in Valencia. Trained panel differences were noted for juice from Hamlin Las+ fruit, especially for symptomatic fruit. Assumptions that juice made from oranges harvested from Huanglongbing (from infection with Liberibacter sp.) affected trees is off-flavored appeared to be generally more true for Hamlin juice than for Midsweet or Valencia, especially for Hamlin juice made from symptomatic fruit. For Midsweet and Valencia, flavor differences between juice made from fruit harvested from diseased or

Application of orange essential oil as an antistaphylococcal agent in a dressing model.

PubMed

Muthaiyan, Arunachalam; Biswas, Debabrata; Crandall, Philip G; Wilkinson, Brian J; Ricke, Steven C

2012-08-16

Staphylococcus aureus is the pathogen most often and prevalently involved in skin and soft tissue infections. In recent decades outbreaks of methicillin-resistant S. aureus (MRSA) have created major problems for skin therapy, and burn and wound care units. Topical antimicrobials are most important component of wound infection therapy. Alternative therapies are being sought for treatment of MRSA and one area of interest is the use of essential oils. With the increasing interest in the use and application of natural products, we screened the potential application of terpeneless cold pressed Valencia orange oil (CPV) for topical therapy against MRSA using an in vitro dressing model and skin keratinocyte cell culture model. The inhibitory effect of CPV was determined by disc diffusion vapor assay for MRSA and vancomycin intermediate-resistant S. aureus (VISA) strains. Antistaphylococcal effect of CPV in an in vitro dressing model was tested on S. aureus inoculated tryptic soya agar plate. Bactericidal effect of CPV on MRSA and VISA infected keratinocyte cells was examined by enumeration of extra- and intra-cellular bacterial cells at different treatment time points. Cytotoxic effects on human skin cells was tested by adding CPV to the keratinocyte (HEK001) cells grown in serum free KSFM media, and observed by phase-contrast microscope. CPV vapour effectively inhibited the MRSA and VISA strains in both disc diffusion vapour assay and in vitro dressing model. Compared to untreated control addition of 0.1% CPV to MRSA infected keratinocyte decreased the viable MRSA cells by 2 log CFU/mL in 1 h and in VISA strain 3 log CFU/mL reduction was observed in 1 h. After 3 h viable S. aureus cells were not detected in the 0.2% CPV treatment. Bactericidal concentration of CPV did not show any cytotoxic effect on the human skin keratinocyte cells in vitro. At lower concentration addition of CPV to keratinocytes infected with MRSA and VISA rapidly killed the bacterial cells without

A beta-galactosidase gene is expressed during mature fruit abscission of 'Valencia' orange (Citrus sinensis).

PubMed

Wu, Zhencai; Burns, Jacqueline K

2004-07-01

beta-galactosidases have been detected in a wide range of plants and are characterized by their ability to hydrolyse terminal non-reducing beta-D-galactosyl residues from beta-D-galactosides. These enzymes have been detected in a wide range of plant organs and tissues. In a search for differentially expressed genes during the abscission process in citrus, sequences encoding beta-galactosidase were identified. Three cDNA fragments of a beta-galactosidase gene were isolated from a cDNA subtraction library constructed from mature fruit abscission zones 48 h after the application of a mature fruit-specific abscission agent, 5-chloro-3-methyl-4-nitro-1H-pyrazole (CMN-pyrazole). Based on sequence information derived from these fragments, a full-length cDNA of 2847 nucleotides (GenBank accession number AY029198) encoding beta-galactosidase was isolated from mature fruit abscission zones by 5'- and 3'-RACE approaches. The beta-galactosidase cDNA encoded a protein of 737 amino acid residues with a calculated molecular weight of 82 kDa. The deduced protein was highly homologous to plant beta-galactosidases expressed in fruit ripening. Southern blot analysis demonstrated that at least two closely related beta-galactosidase genes were present in 'Valencia' orange. Temporal expression patterns in mature fruit abscission zones indicated beta-galactosidase mRNA was detected 48 h after treatment of CMN-pyrazole and ethephon in mature fruit abscission zones. beta-galactosidase transcripts were detected in leaf abscission zones only after ethephon application. The citrus beta-galactosidase was expressed in stamens and petals of fully opened flowers and young fruitlets. The results suggest that this beta-galactosidase may play a role during abscission as well as early growth and development processes in flowers and fruitlets.

Citrus sinensis annotation project (CAP): a comprehensive database for sweet orange genome.

PubMed

Wang, Jia; Chen, Dijun; Lei, Yang; Chang, Ji-Wei; Hao, Bao-Hai; Xing, Feng; Li, Sen; Xu, Qiang; Deng, Xiu-Xin; Chen, Ling-Ling

2014-01-01

Citrus is one of the most important and widely grown fruit crop with global production ranking firstly among all the fruit crops in the world. Sweet orange accounts for more than half of the Citrus production both in fresh fruit and processed juice. We have sequenced the draft genome of a double-haploid sweet orange (C. sinensis cv. Valencia), and constructed the Citrus sinensis annotation project (CAP) to store and visualize the sequenced genomic and transcriptome data. CAP provides GBrowse-based organization of sweet orange genomic data, which integrates ab initio gene prediction, EST, RNA-seq and RNA-paired end tag (RNA-PET) evidence-based gene annotation. Furthermore, we provide a user-friendly web interface to show the predicted protein-protein interactions (PPIs) and metabolic pathways in sweet orange. CAP provides comprehensive information beneficial to the researchers of sweet orange and other woody plants, which is freely available at http://citrus.hzau.edu.cn/.

Osage orange (Maclura pomifera L) seed oil poly(alpha-hydroxydibutylamine) triglycerides: Synthesis and characterization

USDA-ARS?s Scientific Manuscript database

Milled Osage orange seeds (Maclura pomifera (Raf.) Schneid) were Soxhlet extracted with hexane, and portions of the extract were treated with activated carbon before solvent removal. The crude oil was winterized and degummed by centrifugation at low temperature. Decantation of the centrifuge gave an...

Modification of carotenoid levels by abscission agents and expression of carotenoid biosynthetic genes in 'valencia' sweet orange.

PubMed

Alferez, Fernando; Pozo, Luis V; Rouseff, Russell R; Burns, Jacqueline K

2013-03-27

The effect of 5-chloro-3-methyl-4-nitro-1H-pyrazole (CMNP) and ethephon on peel color, flavedo carotenoid gene expression, and carotenoid accumulation was investigated in mature 'Valencia' orange ( Citrus sinensis L. Osbeck) fruit flavedo at three maturation stages. Abscission agent application altered peel color. CMNP was more effective than ethephon in promoting green-to-red (a) and blue-to-yellow (b) color at the middle and late maturation stages and total carotenoid changes at all maturation stages. Altered flow of carotenoid precursors during maturation due to abscission agents was suggested by changes in phytoene desaturase (Pds) and ζ-carotene desaturase (Zds) gene expression. However, each abscission agent affected downstream expression differentially. Ethephon application increased β-carotene hydroxilase (β-Chx) transcript accumulation 12-fold as maturation advanced from the early to middle and late stages. CMNP markedly increased β- and ε-lycopene cyclase (Lcy) transcript accumulation 45- and 15-fold, respectively, at midmaturation. Patterns of carotenoid accumulation in flavedo were supported in part by gene expression changes. CMNP caused greater accumulation of total flavedo carotenoids at all maturation stages when compared with ethephon or controls. In general, CMNP treatment increased total red carotenoids more than ethephon or the control but decreased total yellow carotenoids at each maturation stage. In control fruit flavedo, total red carotenoids increased and yellow carotenoids decreased as maturation progressed. Trends in total red carotenoids during maturation were consistent with measured a values. Changes in carotenoid accumulation and expression patterns in flavedo suggest that regulation of carotenoid accumulation is under transcriptional, translational, and post-translational control.

In vitro effects of citrus oils against Mycobacterium tuberculosis and non-tuberculous Mycobacteria of clinical importance.

PubMed

Crandall, Philip G; Ricke, Steven C; O'Bryan, Corliss A; Parrish, Nicole M

2012-01-01

We evaluated the in vitro activity of citrus oils against Mycobacterium tuberculosis and other non-tuberculous Mycobacterium species. Citrus essential oils were tested against a variety of Mycobacterium species and strains using the BACTEC radiometric growth system. Cold pressed terpeneless Valencia oil (CPT) was further tested using the Wayne model of in vitro latency. Exposure of M. tuberculosis and M. bovis BCG to 0.025 % cold pressed terpeneless Valencia orange oil (CPT) resulted in a 3-log decrease in viable counts versus corresponding controls. Inhibition of various clinical isolates of the M. avium complex and M. abscessus ranged from 2.5 to 5.2-logs. Some species/strains were completely inhibited in the presence of CPT including one isolate each of the following: the M. avium complex, M. chelonae and M. avium subsp. paratuberculosis. CPT also inhibited the growth of BCG more than 99 % in an in vitro model of latency which mimics anaerobic dormancy thought to occur in vivo. The activity of CPT against drug-resistant strains of the M. avium complex and M. abscessus suggest that the mechanism of action for CPT is different than that of currently available drugs. Inhibition of latently adapted bacilli offers promise for treatment of latent infections of MTB. These results suggest that the antimycobacterial properties of CPT warrant further study to elucidate the specific mechanism of action and clarify the spectrum of activity.

Resistance to pathogens in terpene down-regulated orange fruits inversely correlates with the accumulation of D-limonene in peel oil glands.

PubMed

Rodríguez, Ana; Shimada, Takehiko; Cervera, Magdalena; Redondo, Ana; Alquézar, Berta; Rodrigo, María Jesús; Zacarías, Lorenzo; Palou, Lluís; López, María M; Peña, Leandro

2015-01-01

Volatile organic compounds (VOCs) are secondary metabolites acting as a language for the communication of plants with the environment. In orange fruits, the monoterpene D-limonene accumulates at very high levels in oil glands from the peel. Drastic down-regulation of D-limonene synthase gene expression in the peel of transgenic oranges harboring a D-limonene synthase transgene in antisense (AS) configuration altered the monoterpene profile in oil glands, mainly resulting in reduced accumulation of D-limonene. This led to fruit resistance against Penicillium digitatum (Pd), Xanthomonas citri subsp. citri (Xcc) and other specialized pathogens. Here, we analyze resistance to pathogens in independent AS and empty vector (EV) lines, which have low, medium or high D-limonene concentrations and show that the level of resistance is inversely related to the accumulation of D-limonene in orange peels, thus explaining the need of high D-limonene accumulation in mature oranges in nature for the efficient attraction of specialized microorganism frugivores.

Resistance to pathogens in terpene down-regulated orange fruits inversely correlates with the accumulation of D-limonene in peel oil glands

PubMed Central

Rodríguez, Ana; Shimada, Takehiko; Cervera, Magdalena; Redondo, Ana; Alquézar, Berta; Rodrigo, María Jesús; Zacarías, Lorenzo; Palou, Lluís; López, María M; Peña, Leandro

2015-01-01

Volatile organic compounds (VOCs) are secondary metabolites acting as a language for the communication of plants with the environment. In orange fruits, the monoterpene D-limonene accumulates at very high levels in oil glands from the peel. Drastic down-regulation of D-limonene synthase gene expression in the peel of transgenic oranges harboring a D-limonene synthase transgene in antisense (AS) configuration altered the monoterpene profile in oil glands, mainly resulting in reduced accumulation of D-limonene. This led to fruit resistance against Penicillium digitatum (Pd), Xanthomonas citri subsp. citri (Xcc) and other specialized pathogens. Here, we analyze resistance to pathogens in independent AS and empty vector (EV) lines, which have low, medium or high D-limonene concentrations and show that the level of resistance is inversely related to the accumulation of D-limonene in orange peels, thus explaining the need of high D-limonene accumulation in mature oranges in nature for the efficient attraction of specialized microorganism frugivores. PMID:26023857

21 CFR 146.145 - Orange juice from concentrate.

Code of Federal Regulations, 2011 CFR

2011-04-01

... sweetening ingredients referred to in paragraph (a) of this section are sugar, sugar sirup, invert sugar... Juices and Beverages § 146.145 Orange juice from concentrate. (a) Orange juice from concentrate is the...), orange oil, orange pulp, and one or more of the sweetening ingredients listed in paragraph (b) of this...

21 CFR 146.145 - Orange juice from concentrate.

Code of Federal Regulations, 2010 CFR

2010-04-01

... sweetening ingredients referred to in paragraph (a) of this section are sugar, sugar sirup, invert sugar... Juices and Beverages § 146.145 Orange juice from concentrate. (a) Orange juice from concentrate is the...), orange oil, orange pulp, and one or more of the sweetening ingredients listed in paragraph (b) of this...

21 CFR 146.145 - Orange juice from concentrate.

Code of Federal Regulations, 2012 CFR

2012-04-01

... sweetening ingredients referred to in paragraph (a) of this section are sugar, sugar sirup, invert sugar... Juices and Beverages § 146.145 Orange juice from concentrate. (a) Orange juice from concentrate is the...), orange oil, orange pulp, and one or more of the sweetening ingredients listed in paragraph (b) of this...

21 CFR 146.145 - Orange juice from concentrate.

Code of Federal Regulations, 2013 CFR

2013-04-01

... sweetening ingredients referred to in paragraph (a) of this section are sugar, sugar sirup, invert sugar... Juices and Beverages § 146.145 Orange juice from concentrate. (a) Orange juice from concentrate is the...), orange oil, orange pulp, and one or more of the sweetening ingredients listed in paragraph (b) of this...

21 CFR 146.145 - Orange juice from concentrate.

Code of Federal Regulations, 2014 CFR

2014-04-01

... sweetening ingredients referred to in paragraph (a) of this section are sugar, sugar sirup, invert sugar... Juices and Beverages § 146.145 Orange juice from concentrate. (a) Orange juice from concentrate is the...), orange oil, orange pulp, and one or more of the sweetening ingredients listed in paragraph (b) of this...

Postharvest response of oranges of ethylene

DOE Office of Scientific and Technical Information (OSTI.GOV)

Aharoni, Y.; Lattar, F.S.; Monselise, S.P.

1969-01-01

Since the effect of ethylene on the respiration rate of young unripe oranges has not yet been described, experiments were carried out to study the respiratory response of such fruits to ethylene. Oranges (Citrus sinensis (L.) Osbeck) of the Washington navel, Shamouti and Valencia varieties, growing on a sandy loam near Rehovot, Israel, were picked in June, July, September, December and January. Immediately after picking, they were weighed, placed in glass jars, and stored at 20/sup 0/. Each experiment comprised 12 jars, 4 for each variety. A constant stream (200 ml/min) of air containing 20 ppm of ethylene was runmore » through the jars. The respiration rate was measured by the amount of CO/sub 2/ evolved by the fruits, and based on a fresh weight unit. The results show that in all 3 varieties, ethylene caused an immediate rise in respiration rate, at all stages of development tested, regardless of whether it was applied from the beginning of the test or after a certain period of storage in air. The pattern of response to ethylene of young unripe oranges (picked in June, July, and September) was therefore similar to that of ripe ones (picked in December and January).« less

Fungal decay and shelf life of oranges coated with chitosan and bergamot, thyme, and tea tree essential oils.

PubMed

Cháfer, M; Sánchez-González, L; González-Martínez, Ch; Chiralt, A

2012-08-01

Chitosan coatings, containing or not essential oils (bergamot, thyme and tea tree oil), were applied to oranges (cv. Navel Powell). Antifungal effect was evaluated by applying coatings before and after inoculating the fruit with Penicillium italicum CECT 2294 (10(5) spores/mL), preventive and curative treatments, respectively. The effect of coatings on the quality parameters (acidity, pH, soluble solids, juice percentage, weight loss, firmness, color parameters, and respiration rate) was controlled for the different oranges samples throughout the cold storage time. Preventive antimicrobial treatments with coatings containing tea tree oil were the most effective with a reduction of the microbial growth (expressed as the percentage of infected samples) of 50%, as compared to the uncoated samples. The coatings did not lead to any relevant changes in the development of the sample quality parameters throughout the cold storage, except for a slightly reduced loss of both weight and firmness when the coatings contained bergamot oil. Results of this study are a useful tool for the development of new environmental friendly and healthier commercial applications in the control of the main postharvest fungal decay of citrus fruits. © 2012 Institute of Food Technologists®

Production, Characterization, and Stability of Orange or Eucalyptus Essential Oil/β-Cyclodextrin Inclusion Complex.

PubMed

Kringel, Dianini Hüttner; Antunes, Mariana Dias; Klein, Bruna; Crizel, Rosane Lopes; Wagner, Roger; de Oliveira, Roberto Pedroso; Dias, Alvaro Renato Guerra; Zavareze, Elessandra da Rosa

2017-11-01

The aim of this study was to produce and characterize inclusion complexes (IC) between β-cyclodextrin (β-CD) and orange essential oil (OEO) or eucalyptus essential oil (EEO), and to compare these with their pure compounds and physical mixtures. The samples were evaluated by chemical composition, morphology, thermal stability, and volatile compounds by static headspace-gas chromatography (SH-GC). Comparing the free essential oil and physical mixture with the inclusion complex, of both essential oils (OEO and EEO), it was observed differences occurred in the chemical composition, thermal stability, and morphology. These differences show that there was the formation of the inclusion complex and demonstrate the necessity of the precipitation method used to guarantee the interaction between β-CD and essential oils. The slow loss of the volatile compounds from both essential oils, when complexed with β-CD, showed a higher stability when compared with their physical mixtures and free essential oils. Therefore, the results showed that the chemical composition, molecular size, and structure of the essential oils influence the characteristics of the inclusion complexes. The application of the β-CD in the formation of inclusion complexes with essential oils can expand the potential applications in foods. © 2017 Institute of Food Technologists®.

Potent Inhibition of Pseudogymnoascus destructans, the Causative Agent of White-Nose Syndrome in Bats, by Cold-Pressed, Terpeneless, Valencia Orange Oil.

PubMed

Boire, Nicholas; Zhang, Sean; Khuvis, Joshua; Lee, Rick; Rivers, Jennifer; Crandall, Philip; Keel, M Kevin; Parrish, Nicole

2016-01-01

The causative agent of White-nose Syndrome (WNS), Pseudogymnoascus destructans, has been shown to be fatal to several species of bats in North America. To date, no compounds or chemical control measures have been developed which eliminates the growth of the fungus in the environment or in affected animals. In the current study, we evaluated the activity of cold-pressed, terpeneless orange oil (CPT) against multiple isolates of P. destructans in vitro. For all assays, a modified Kirby-Bauer disk diffusion assay was used. Standardized spore suspensions were prepared, adjusted to a specific optical density, and used to plate fungal lawns. Plates were incubated at either 15°C or 4°C for up to 6 months and checked at regular intervals for growth. Once controls had grown, zones of inhibition were measured (mm) on test plates and compared to those obtained using current antifungal drugs. All P. destructans isolates were completely inhibited by 100% CPT (10 μL) at 1 month of incubation regardless of temperature (4°C and 15°C). Complete inhibition persisted up to 6 months following a single exposure at this concentration. Of the standard antifungals, only amphotericin B demonstrated any activity, resulting in zone diameters ranging from 58 mm to 74 mm. CPT, at the highest concentration tested (100%), had no significant effect against a variety of other environmental organisms including various filamentous fungi, bacteria and aerobic actinomycetes. Given that CPT is relatively non-toxic, the possibility exists that the all-natural, mixture could be used as an environmental pre-treatment to eradicate P. destructans from bat habitats. Additional studies are needed to assess any undesirable effects of CPT on bat behavior and health and overall impacts on other members of the interconnected ecosystem(s).

Potent Inhibition of Pseudogymnoascus destructans, the Causative Agent of White-Nose Syndrome in Bats, by Cold-Pressed, Terpeneless, Valencia Orange Oil

PubMed Central

Boire, Nicholas; Zhang, Sean; Khuvis, Joshua; Lee, Rick; Rivers, Jennifer; Crandall, Philip; Keel, M. Kevin; Parrish, Nicole

2016-01-01

The causative agent of White-nose Syndrome (WNS), Pseudogymnoascus destructans, has been shown to be fatal to several species of bats in North America. To date, no compounds or chemical control measures have been developed which eliminates the growth of the fungus in the environment or in affected animals. In the current study, we evaluated the activity of cold-pressed, terpeneless orange oil (CPT) against multiple isolates of P. destructans in vitro. For all assays, a modified Kirby-Bauer disk diffusion assay was used. Standardized spore suspensions were prepared, adjusted to a specific optical density, and used to plate fungal lawns. Plates were incubated at either 15°C or 4°C for up to 6 months and checked at regular intervals for growth. Once controls had grown, zones of inhibition were measured (mm) on test plates and compared to those obtained using current antifungal drugs. All P. destructans isolates were completely inhibited by 100% CPT (10 μL) at 1 month of incubation regardless of temperature (4°C and 15°C). Complete inhibition persisted up to 6 months following a single exposure at this concentration. Of the standard antifungals, only amphotericin B demonstrated any activity, resulting in zone diameters ranging from 58 mm to 74 mm. CPT, at the highest concentration tested (100%), had no significant effect against a variety of other environmental organisms including various filamentous fungi, bacteria and aerobic actinomycetes. Given that CPT is relatively non-toxic, the possibility exists that the all-natural, mixture could be used as an environmental pre-treatment to eradicate P. destructans from bat habitats. Additional studies are needed to assess any undesirable effects of CPT on bat behavior and health and overall impacts on other members of the interconnected ecosystem(s). PMID:26849057

Effect of second cooling on the chemical components of essential oils from orange peel (Citrus sinensis).

PubMed

Chen, Yulong; Wu, Jijun; Xu, Yujuan; Fu, Manqing; Xiao, Gengsheng

2014-09-03

A second cooling was added to the oil collectors of an improved Clevenger-type apparatus (ICT) to investigate the thermal reaction of essential oils from orange peel compared to a traditional Clevenger-type apparatus (CT). The results demonstrated the yield rate of essential oil from ICT was significantly higher (p < 0.05) than that from CT. The major components of the essential oils consisted of monoterpenes, such as d-limonene, β-myrcene, β-pinene, γ-terpinene, α-pinene. Interestingly, ICT prevented the thermal reaction-the transformation of β-myrcene to β-thujene-and reduced the oxidation on α-pinene and β-pinene of the essential oil in comparison to CT. In addition, the yield rate of γ-terpinene can also be improved via ICT compared to CT. Thus, ICT is an effective improvement to traditional CT.

Efficacy of ultrasonic activation of NaOCl and orange oil in removing filling material from mesial canals of mandibular molars with and without isthmus

PubMed Central

Barreto, Mirela Sangoi; da Rosa, Ricardo Abreu; Santini, Manuela Favarin; Cavenago, Bruno Cavalini; Duarte, Marco Antônio Húngaro; Bier, Carlos Alexandre Souza; Só, Marcos Vinícius Reis

2016-01-01

ABSTRACT Objectives The aim of this study was to evaluate the volume of remaining filling material after passive ultrasonic irrigation (PUI) of sodium hypochlorite (NaOCl) and orange oil in mesial canals of mandibular molars, with and without isthmus. Material and Methods Thirty mesial roots of mandibular molars were divided according to the presence or absence of isthmus. Canals were prepared and filled (Micro-CT #1). Filling was removed using rotary instruments, and specimens were sub-divided into three groups according to the irrigation procedures: Conventional – conventional irrigation with NaOCl, PUI/NaOCl – PUI of NaOCl (three activations, 20 seconds each), and PUI/orange oil – PUI of orange oil (Micro-CT#2). Specimens were enlarged using the X2 and X3 ProTaper Next instruments and submitted to the same irrigation protocols (Micro-CT #3). Results No differences were found between the experimental groups in each stage of assessment (P>0.05). The volume of residual filling material was similar to those in Micro-CT #2 and Micro-CT #3, but lower than those observed in Micro-CT #1 (P<0.05). When groups were pooled according to the presence or absence of an isthmus, volume of residual filling material was higher in specimens presenting isthmus (P<0.05). Conclusions PUI of NaOCl or orange oil did not improve filling removal. Isthmus consists in an anatomical obstacle that impairs the removal of filling material. PMID:26200525

Nanoemulsion of orange oil with non ionic surfactant produced emulsion using ultrasonication technique: evaluating against food spoilage yeast

NASA Astrophysics Data System (ADS)

Sugumar, Saranya; Singh, Sanjay; Mukherjee, Amitava; Chandrasekaran, N.

2016-01-01

In recent years, food industries have shown great interest in developing nanoemulsion (NE) using essential oils (EOs) to prevent food spoilage caused by microorganisms. The hydrophobic properties of EOs have lead to reduced solubilization effect of food, which in turn, created a negative impact on the quality of food and its antimicrobial efficacy. Focusing this issue, we attempted a unique NE preparation using orange oil, Tween 80 (organic phase) and water (aqueous phase) by sonication technique. Based on thermodynamic stability studies, the effective diameter was reported to be in the size range from 20 to 30 nm. Saccharomyces cerevisiae was used in testing the anti-yeast effect. Their activity was studied in both growth medium and apple juice. The minimum inhibitory concentration of this NE was determined using broth dilution method. At 2 μl/ml, orange oil NE demonstrated inhibition of tested microorganisms. The kinetics of killing curve, have shown that the NE treated cells had lost its viability within 30 min of interaction. Also, SEM image revealed that the treated cells became distorted in comparison to their control cells. NE treated apple juice showed complete loss of viability even on dilution as compared to their controls.

Citric Acid Production from Orange Peel Wastes by Solid-State Fermentation

PubMed Central

Torrado, Ana María; Cortés, Sandra; Manuel Salgado, José; Max, Belén; Rodríguez, Noelia; Bibbins, Belinda P.; Converti, Attilio; Manuel Domínguez, José

2011-01-01

Valencia orange (Citrus sinensis) peel was employed in this work as raw material for the production of citric acid (CA) by solid-state fermentation (SSF) of Aspergillus niger CECT-2090 (ATCC 9142, NRRL 599) in Erlenmeyer flasks. To investigate the effects of the main operating variables, the inoculum concentration was varied in the range 0.5·103 to 0.7·108 spores/g dry orange peel, the bed loading from 1.0 to 4.8 g of dry orange peel (corresponding to 35-80 % of the total volume), and the moisture content between 50 and 100 % of the maximum water retention capacity (MWRC) of the material. Moreover, additional experiments were done adding methanol or water in different proportions and ways. The optimal conditions for CA production revealed to be an inoculum of 0.5·106 spores/g dry orange peel, a bed loading of 1.0 g of dry orange peel, and a humidification pattern of 70 % MWRC at the beginning of the incubation with posterior addition of 0.12 mL H2O/g dry orange peel (corresponding to 3.3 % of the MWRC) every 12 h starting from 62 h. The addition of methanol was detrimental for the CA production. Under these conditions, the SSF ensured an effective specific production of CA (193 mg CA/g dry orange peel), corresponding to yields of product on total initial and consumed sugars (glucose, fructose and sucrose) of 376 and 383 mg CA/g, respectively. These results, which demonstrate the viability of the CA production by SSF from orange peel without addition of other nutrients, could be of interest to possible, future industrial applications. PMID:24031646

Host status of grapefruit and Valencia oranges for Anastrepha serpentina and Anastrepha ludens

USDA-ARS?s Scientific Manuscript database

Anastrepha serpentina, known as the zapote fly or serpentine fruit fly, is occasionally captured in the Rio Grande Valley of Texas. Lists of host plants for this species include several species of citrus, such as oranges and grapefruit. Current regulatory procedures require quarantines and treatme...

Determination of volatile, phenolic, organic acid and sugar components in a Turkish cv. Dortyol (Citrus sinensis L. Osbeck) orange juice.

PubMed

Kelebek, Hasim; Selli, Serkan

2011-08-15

Orange flavour is the results of a natural combination of volatile compounds in a well-balanced system including sugars, acids and phenolic compounds. This paper reports the results of the first determination of aroma, organic acids, sugars, and phenolic components in Dortyol yerli orange juices. A total of 58 volatile components, including esters (nine), terpenes (19), terpenols (13), aldehydes (two), ketones (three), alcohols (four) and acids (eight) were identified and quantified in Dortyol yerli orange juice by GC-FID and GC-MS. Organic acids, sugars and phenolic compositions were also determined by HPLC methods. The major organic acid and sugar found were citric acid and sucrose, respectively. With regard to phenolics, 14 compounds were identified and quantified in the orange juice. Terpenes and terpenols were found as the main types of volatile components in Dortyol yerli orange juice. In terms of aroma contribution to orange juice, 12 compounds were prominent based on the odour activity values (OAVs). The highest OAV values were recorded for ethyl butanoate, nootkatone, linalool and DL-limonene. When we compare the obtained results of cv. Dortyol orange juice with the other orange juice varieties, the composition of Dortyol orange juice was similar to Valencia and Navel orange juices. Copyright © 2011 Society of Chemical Industry.

Carbon isotope ratios of selected volatiles in Citrus sinensis and in orange-flavoured food.

PubMed

Schipilliti, Luisa; Bonaccorsi, Ivana; Cotroneo, Antonella; Dugo, Paola; Mondello, Luigi

2015-11-01

Twenty genuine samples of industrially cold-pressed sweet orange essential oils, were analysed by gas chromatography-combustion-isotope ratio mass spectrometry to determine the values of the carbon isotope ratios (δ(13)C(VPDB)) of selected volatiles and assess the corresponding range of authenticity. Successively, four commercial orange-flavoured products were analysed under identical conditions to evaluate the authenticity of the orange flavour. The samples were extracted by solid-phase microextraction under optimised conditions. The evaluation was performed by using an internal standard procedure to neglect the contribution due to the original environment to the isotopic abundance of (13)C. The composition of the volatile fraction of the essential oils and of the flavoured products was determined by gas chromatography coupled to mass spectrometry with linear retention indices, and by gas chromatography with a flame ionisation detector. The δ(13)C(VPDB) values of seven secondary metabolites determined here were successfully used to characterise genuine orange essential oil. These values were used to evaluate the quality of orange-flavoured products, revealing the presence of compounds of different origin, not compatible with the values of genuine orange secondary metabolites. This study provides the range of authenticity of δ(13)C(VPDB) of seven different secondary metabolites in sweet orange genuine essential oil, useful for evaluating the genuineness of orange flavour. In accord with a previous study on different essential oils, the values determined here can be successfully applied for the evaluation of a large number of flavoured food stuffs and correlated with their origins. © 2014 Society of Chemical Industry.

Classification of Bitter Orange Essential Oils According to Fruit Ripening Stage by Untargeted Chemical Profiling and Machine Learning.

PubMed

Taghadomi-Saberi, Saeedeh; Mas Garcia, Sílvia; Allah Masoumi, Amin; Sadeghi, Morteza; Marco, Santiago

2018-06-13

The quality and composition of bitter orange essential oils (EOs) strongly depend on the ripening stage of the citrus fruit. The concentration of volatile compounds and consequently its organoleptic perception varies. While this can be detected by trained humans, we propose an objective approach for assessing the bitter orange from the volatile composition of their EO. The method is based on the combined use of headspace gas chromatography⁻mass spectrometry (HS-GC-MS) and artificial neural networks (ANN) for predictive modeling. Data obtained from the analysis of HS-GC-MS were preprocessed to select relevant peaks in the total ion chromatogram as input features for ANN. Results showed that key volatile compounds have enough predictive power to accurately classify the EO, according to their ripening stage for different applications. A sensitivity analysis detected the key compounds to identify the ripening stage. This study provides a novel strategy for the quality control of bitter orange EO without subjective methods.

Fresh squeezed orange juice odor: a review.

PubMed

Perez-Cacho, Pilar Ruiz; Rouseff, Russell L

2008-08-01

Fresh orange juice is a highly desirable but unstable product. This review examines analytical findings, odor activity, and variations due to cultivar, sampling methods, manner of juicing, plus possible enzymatic and microbial artifacts. Initial attempts to characterize orange juice odor were based on volatile quantitation and overemphasized the importance of high concentration volatiles. Although over 300 volatiles have been reported from GC-MS analytical studies, this review presents 36 consensus aroma active components from GC-olfactometry studies consisting of 14 aldehydes, 7 esters, 5 terpenes, 6 alcohols, and 4 ketones. Most are trace (microg/L) components. (+)-Limonene is an essential component in orange juice odor although its exact function is still uncertain. Total amounts of volatiles in mechanically squeezed juices are three to 10 times greater than hand-squeezed juices because of elevated peel oil levels. Elevated peel oil changes the relative proportion of several key odorants. Odor active components from solvent extraction studies differ from those collected using headspace techniques as they include volatiles with low vapor pressure such as vanillin. Some reported odorants such as 2,3-butanedione are microbial contamination artifacts. Orange juice odor models confirm that fresh orange aroma is complex as the most successful models contain 23 odorants.

The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck).

PubMed

Boscariol, R L; Almeida, W A B; Derbyshire, M T V C; Mourão Filho, F A A; Mendes, B M J

2003-09-01

A new method for obtaining transgenic sweet orange plants was developed in which positive selection (Positech) based on the Escherichia coli phosphomannose-isomerase (PMI) gene as the selectable marker gene and mannose as the selective agent was used. Epicotyl segments from in vitro-germinated plants of Valencia, Hamlin, Natal and Pera sweet oranges were inoculated with Agrobacterium tumefaciens EHA101-pNOV2116 and subsequently selected on medium supplemented with different concentrations of mannose or with a combination of mannose and sucrose as a carbon source. Genetic transformation was confirmed by PCR and Southern blot. The transgene expression was evaluated using a chlorophenol red assay and isoenzymes. The transformation efficiency rate ranged from 3% to 23.8%, depending on cultivar. This system provides an efficient manner for selecting transgenic sweet orange plants without using antibiotics or herbicides.

The Effect of Aromatherapy with the Essential Oil of Orange on Pain and Vital Signs of Patients with Fractured Limbs Admitted to the Emergency Ward: A Randomized Clinical Trial

PubMed Central

Hekmatpou, Davood; Pourandish, Yasaman; Farahani, Pouran Varvani; Parvizrad, Ramin

2017-01-01

Background and Objective: Pain is an emotional and unpleasant experience associated with actual or potential tissue damage. The literature shows no study on the effect of aromatherapy with the essential oil of orange on unpleasant feelings of patients with fractured limbs. In this regard, this paper aims at studying the effect of aromatherapy with the essential oil of orange on patients with fractured limbs admitted to the emergency ward. Methods: Sixty patients admitted to the emergency ward of Vali-e-Asr Hospital were selected by purposive sampling method and then were divided into two groups of control and experiment by block method. This study was done in one shift work (morning or afternoon). Four drops of the orange oil were poured on a pad and were pinned with a plastic pin to the patient's collar, about 20 cm distant from head. The old pad was replaced by the new one every 1 h. The patients' pain and vital signs were checked every 1 h for at last 6 h. The data were analyzed by SPSS Version 21. Results: Forty (66.7%) patients were male and twenty (33.3%) were female. Their age average was 37.93 ± 18.19 years old. The most fractured cases were in the scapular (11 patients [18.3%]). Friedman test showed that pain in the experiment group (P = 0.0001) decreased significantly rather than the control group (0.339). However, in vital signs, there could be found that no significant change between the two groups was seen. Conclusion: Aromatherapy with orange oil can relieve pain in patients with fractured limbs but has no effect on their vital signs. Therefore, aromatherapy with orange oil can be used as a complementary medicine in these patients. PMID:29123351

An HPLC-MS characterization of the changes in sweet orange leaf metabolite profile following infection by the bacterial pathogen Candidatus Liberibacter asiaticus.

PubMed

Hijaz, Faraj M; Manthey, John A; Folimonova, Svetlana Y; Davis, Craig L; Jones, Shelley E; Reyes-De-Corcuera, José I

2013-01-01

Huanglongbing (HLB) presumably caused by Candidatus Liberibacter asiaticus (CLas) threatens the commercial U.S. citrus crop of an annual value of $3 billion. The earliest shift in metabolite profiles of leaves from greenhouse-grown sweet orange trees infected with Clas, and of healthy leaves, was characterized by HPLC-MS concurrently with PCR testing for the presence of Clas bacteria and observation of disease symptoms. Twenty, 8-month-old 'Valencia' and 'Hamlin' trees were grafted with budwood from PCR-positive HLB source trees. Five graft-inoculated trees of each variety and three control trees were sampled biweekly and analyzed by HPLC-MS and PCR. Thirteen weeks after inoculation, Clas was detected in newly growing flushes in 33% and 55% of the inoculated 'Hamlin' and 'Valencia' trees, respectively. Inoculated trees remained asymptomatic in the first 20 weeks, but developed symptoms 30 weeks after grafting. No significant differences in the leaf metabolite profiles were detected in Clas-infected trees 23 weeks after inoculation. However, 27 weeks after inoculation, differences in metabolite profiles between control leaves and those of Clas-infected trees were evident. Affected compounds were identified with authentic standards or structurally classified by their UV and mass spectra. Included among these compounds are flavonoid glycosides, polymethoxylated flavones, and hydroxycinnamates. Four structurally related hydroxycinnamate compounds increased more than 10-fold in leaves from 'Hamlin' and 'Valencia' sweet orange trees in response to Clas infection. Possible roles of these hydroxycinnamates as plant defense compounds against the Clas infection are discussed.

The effect of aromatherapy by essential oil of orange on anxiety during labor: A randomized clinical trial

PubMed Central

Rashidi-Fakari, Fahimeh; Tabatabaeichehr, Mahbubeh; Mortazavi, Hamed

2015-01-01

Background: Labor is a stressful situation that may have an adverse impact. Aromatherapy is a method to control anxiety and stress of women. This study was conducted to investigate the effect of aromatherapy using essential oil of orange on women's anxiety during labor. Materials and Methods: In this clinical trial study, 100 women during labor were randomly assigned to two groups: intervention group and control group. The women in the intervention group were exposed to orange essential oil, but the women in the control group were exposed to distilled water. The women's anxiety was assessed using the Spielberger inventory. Moreover, physiological parameters such as systolic and diastolic blood pressure, respiration and pulse rates were assessed in all the women before and 20 min after the intervention. The data were analyzed by Chi-square, Wilcoxon, paired t-test, and Mann–Whitney U test. Data were evaluated with the SPSS 16 program. The significance level of P < 0.05 was considered. Results: The level of anxiety of women in both intervention (P = 0.03) and control (P = 0.003) groups reduced after the intervention. However, the reduction was more in the intervention group (difference in anxiety scores after the intervention in comparison to before intervention = −3.08) in comparison to the control group (score = −1.14). No significant change was found in the physiological parameters of women in the intervention group after the intervention. Conclusions: Aromatherapy is a noninvasive and effective method to help women overcome their anxiety during labor. Orange scent can be useful in childbirth units to help women who are experiencing stress in labor. PMID:26793249

Effect of the concentration of essential oil on orange peel waste biomethanization: Preliminary batch results.

PubMed

Calabrò, P S; Pontoni, L; Porqueddu, I; Greco, R; Pirozzi, F; Malpei, F

2016-02-01

The cultivation of orange (Citrus×sinensis) and its transformation is a major industry in many countries in the world, it leads to the production of about 25-30Mt of orange peel waste (OPW) per year. Until now many options have been proposed for the management of OPW but although they are technically feasible, in many cases their economic/environmental sustainability is questionable. This paper analyse at lab scale the possibility of using OPW as a substrate for anaerobic digestion. Specific objectives are testing the possible codigestion with municipal biowaste, verifying the effect on methane production of increasingly high concentration of orange essential oil (EO, that is well known to have antioxidant properties that can slower or either inhibit biomass activity) and obtaining information on the behaviour of d-limonene, the main EO component, during anaerobic digestion. The results indicate that OPW can produce up to about 370LnCH4/kgVS in mesophilic conditions and up to about 300LnCH4/kgVS in thermophilic conditions. The presence of increasingly high concentrations of EO temporary inhibits methanogenesis, but according to the results of batch tests, methane production restarts while d-limonene is partially degraded through a pathway that requires its conversion into p-cymene as the main intermediate. Copyright © 2015 Elsevier Ltd. All rights reserved.

Metabolomic approaches for orange origin discrimination by ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry.

PubMed

Díaz, Ramon; Pozo, Oscar J; Sancho, Juan V; Hernández, Félix

2014-08-15

In this work, hybrid quadrupole time-of-flight mass spectrometer (QTOF MS) coupled to ultra high performance liquid chromatography (UHPLC) has been used for biomarkers identification for correct authentication of Valencia (Spain) oranges. Differentiation from foreign Argentinean, Brazilian and South African oranges has been carried out using XCMS application and multivariate analysis to UHPLC-(Q)TOF MS data acquired in both, positive and negative ionisation modes. Several markers have been found and corroborated by analysing two seasons samples. A seasonal independent marker was found and its structure elucidated using accurate mass data and MS(E) fragmentation spectrum information. Empirical formula was searched in Reaxys database applying sub-structure filtering from the fragments obtained. Three possible structures were found and citrusin D, a compound present in sweet oranges, has been identified as the most plausible as it fits better with the product ion scan performed for this compound. As a result of data obtained in this work, citrusin D is suggested as a potential marker to distinguish the geographic origin of oranges. Copyright © 2014 Elsevier Ltd. All rights reserved.

Effect of sweet orange aroma on experimental anxiety in humans.

PubMed

Goes, Tiago Costa; Antunes, Fabrício Dias; Alves, Péricles Barreto; Teixeira-Silva, Flavia

2012-08-01

The objective of this study was to evaluate the potential anxiolytic effect of sweet orange (Citrus sinensis) aroma in healthy volunteers submitted to an anxiogenic situation. Forty (40) male volunteers were allocated to five different groups for the inhalation of sweet orange essential oil (test aroma: 2.5, 5, or 10 drops), tea tree essential oil (control aroma: 2.5 drops), or water (nonaromatic control: 2.5 drops). Immediately after inhalation, each volunteer was submitted to a model of anxiety, the video-monitored version of the Stroop Color-Word Test (SCWT). Psychologic parameters (state-anxiety, subjective tension, tranquilization, and sedation) and physiologic parameters (heart rate and gastrocnemius electromyogram) were evaluated before the inhalation period and before, during, and after the SCWT. Unlike the control groups, the individuals exposed to the test aroma (2.5 and 10 drops) presented a lack of significant alterations (p>0.05) in state-anxiety, subjective tension and tranquillity levels throughout the anxiogenic situation, revealing an anxiolytic activity of sweet orange essential oil. Physiologic alterations along the test were not prevented in any treatment group, as has previously been observed for diazepam. Although more studies are needed to find out the clinical relevance of aromatherapy for anxiety disorders, the present results indicate an acute anxiolytic activity of sweet orange aroma, giving some scientific support to its use as a tranquilizer by aromatherapists.

Flora of the Orange Cliffs of Utah

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shultz, L.M.; Neely, E.E.; Tuhy, J.S.

1987-04-30

The Orange Cliffs area, an area rich in oil sands deposits and defined here as part of the Colorado Plateau floristic province, harbors approximately 209 species in 123 genera and 49 families. Because of the potential of exploitation of the oil sands deposits in the area, a species checklist was made and a discussion of physical and floristic aspects of the region is given here. The flora is compared statistically to the San Rafael Swell flora, which is also a subset of the Colorado Plateau. They define six vegetation types and three edaphic communities; these are described and mapped. Ofmore » eleven endemic plant species in the Orange Cliffs, three are local and rare. Sites for Astragalus nidularius, A. moencoppensis, and Xylorhiza glabriuscula var. linearifolia are discussed and mapped. 24 references, 3 figures, 3 tables.« less

Effect of Abscission Zone Formation on Orange ( Citrus sinensis) Fruit/Juice Quality for Trees Affected by Huanglongbing (HLB).

PubMed

Baldwin, Elizabeth; Plotto, Anne; Bai, Jinhe; Manthey, John; Zhao, Wei; Raithore, Smita; Irey, Mike

2018-03-21

Orange trees affected by huanglongbing (HLB) exhibit excessive fruit drop, and fruit loosely attached to the tree may have inferior flavor. Fruit were collected from healthy and HLB-infected ( Candidatus liberibacter asiaticus) 'Hamlin' and 'Valencia' trees. Prior to harvest, the trees were shaken, fruit that dropped collected, tree-retained fruit harvested, and all fruit juiced. For chemical analyses, sugars and acids were generally lowest in HLB dropped (HLB-D) fruit juice compared to nonshaken healthy (H), healthy retained (H-R), and healthy dropped fruit (H-D) in early season (December) but not for the late season (January) 'Hamlin' or 'Valencia' except for sugar/acid ratio. The bitter limonoids, many flavonoids, and terpenoid volatiles were generally higher in HLB juice, especially HLB-D juice, compared to the other samples. The lower sugars, higher bitter limonoids, flavonoids, and terpenoid volatiles in HLB-D fruit, loosely attached to the tree, contributed to off-flavor, as was confirmed by sensory analyses.

Changes in volatile and non-volatile flavor chemicals of "Valencia" orange juice over the harvest seasons

USDA-ARS?s Scientific Manuscript database

Florida ‘Valencia’ oranges have a wide harvest window covering four months after first reaching the commercial maturity. However, the influence of harvest time on juice flavor chemicals is not well documented with the exception of sugars and acids. Therefore, we investigated the major flavor chemica...

Encapsulation of Polymethoxyflavones in Citrus Oil Emulsion-Based Delivery Systems.

PubMed

Yang, Ying; Zhao, Chengying; Chen, Jingjing; Tian, Guifang; McClements, David Julian; Xiao, Hang; Zheng, Jinkai

2017-03-01

The purpose of this work was to elucidate the effects of citrus oil type on polymethoxyflavone (PMF) solubility and on the physicochemical properties of PMF-loaded emulsion-based delivery systems. Citrus oils were extracted from mandarin, orange, sweet orange, and bergamot. The major constituents were determined by GC/MS: sweet orange oil (97.4% d-limonene); mandarin oil (72.4% d-limonene); orange oil (67.2% d-limonene); and bergamot oil (34.6% linalyl acetate and 25.3% d-limonene). PMF-loaded emulsions were fabricated using 10% oil phase (containing 0.1% w/v nobiletin or tangeretin) and 90% aqueous phase (containing 1% w/v Tween 80) using high-pressure homogenization. Delivery systems prepared using mandarin oil had the largest mean droplet diameters (386 or 400 nm), followed by orange oil (338 or 390 nm), bergamot oil (129 or 133 nm), and sweet orange oil (122 or 126 nm) for nobiletin- or tangeretin-loaded emulsions, respectively. The optical clarity of the emulsions increased with decreasing droplet size due to reduced light scattering. The viscosities of the emulsions (with or without PMFs) were similar (1.3 to 1.4 mPa·s), despite appreciable differences in oil phase viscosity. The loading capacity and encapsulation efficiency of the emulsions depended on carrier oil type, with bergamot oil giving the highest loading capacity. In summary, differences in the composition and physical characteristics of citrus oils led to PMF-loaded emulsions with different encapsulation and physicochemical characteristics. These results will facilitate the rational design of emulsion-based delivery systems for encapsulation of PMFs and other nutraceuticals in functional foods and beverages.

Improved quality of frozen boer goat semen with the addition of sweet orange essential oil on tris yolk and gentamicin extender

NASA Astrophysics Data System (ADS)

Sitepu, S. A.; Zaituni, U.; Jaswandi; Hendri

2018-02-01

This research aimed to determine the extent of frozen semen quality Boer Goat by essential oils of sweet orange peel in tris yolk and gentamicin extender. Research has been conducted at the Laboratory Loka Penelitian Kambing Potong Sei Putih, Deli Serdang, North Sumatra in February 2017. This study used a completely randomized design with 4 treatments and 5 replications. Treatments are 0.25; 0.5; 0.75 and 1% essential oils as additional diluent. The parameters were measured percentage Motility, membrane integrity, acrosome integrity and viability Boer Goat frozen semen. The results showed that the addition of essential oils as diluent semen was significant (P <0.01) in the percentage motility, Viability, membrane integrity and acrosome integrity Boer Goat frozen semen. Motility, membrane integrity, acrosome integrity and viability was significantly higher in all treated groups than the control group. The best results of all treatments In the study was the addition of essential oil as much as 1%.

Effects of organic fertilisation on sweet orange bearing trees

NASA Astrophysics Data System (ADS)

Roccuzzo, Giancarlo; Torrisi, Biagio; Canali, Stefano; Intrigliolo, Francesco

2010-05-01

In a study realised over a five year period (2001-2006) on orange bearing trees [Citrus sinensis (L.) Osbeck] cv. ‘Valencia late', grafted on sour orange (C. aurantium L.), four fertiliser treatments were applied: citrus by-products compost (CB), poultry manure (PM), livestock waste compost (LW) and mineral fertiliser (MF), as control. The trees, with the exception of MF treatment, were organically grown since 1994 in the experimental farm of CRA-ACM in Lentini, Sicily, and received the same N input every year. The research objectives were to evaluate the effect of long term repeated organic fertilisers application on i) soil fertility; ii) citrus bearing trees nutritional status by means of leaf analysis and iii) yield and fruit quality, determining parameters currently utilized to evaluate sweet orange production either for fresh consumption and processing. The CB treatment showed significantly higher values of Corg in soil than MF treatment (about 30%). Corg in PM and LW treatments was higher than MF treatment (13% and 20%, respectively), but these differences were not statistically significant either from the control treatment nor from the soil fertilised with CB. Similar trend was showed by the humic and fulvic C being the values of the CB treatment significantly higher than the control. PM and LW treatments had intermediate values, without statistical significance. The long term addition to soil of a quality compost (CB) with high C/N ratio increased the level of nutrients wich usually show low availability for citrus plants (P, Fe, Zn, Mn), as demonstrated by leaf analysis. No significant difference was noticed as far as yield was concerned, whereas CB treatment enhanced some fruit quality parameters.

7 CFR 906.13 - Variety or varieties.

Code of Federal Regulations, 2013 CFR

2013-01-01

...) Navel oranges; (b) Early and Midseason oranges, except Navel oranges; (c) Valencia and similar late type oranges; (d) White seeded grapefruit; (e) White seedless grapefruit; (f) Pink and red seeded grapefruit...

7 CFR 906.13 - Variety or varieties.

Code of Federal Regulations, 2014 CFR

2014-01-01

...) Navel oranges; (b) Early and Midseason oranges, except Navel oranges; (c) Valencia and similar late type oranges; (d) White seeded grapefruit; (e) White seedless grapefruit; (f) Pink and red seeded grapefruit...

7 CFR 906.13 - Variety or varieties.

Code of Federal Regulations, 2011 CFR

2011-01-01

...) Navel oranges; (b) Early and Midseason oranges, except Navel oranges; (c) Valencia and similar late type oranges; (d) white seeded grapefruit; (e) white seedless grapefruit; (f) pink and red seeded grapefruit...

7 CFR 906.13 - Variety or varieties.

Code of Federal Regulations, 2012 CFR

2012-01-01

...) Navel oranges; (b) Early and Midseason oranges, except Navel oranges; (c) Valencia and similar late type oranges; (d) white seeded grapefruit; (e) white seedless grapefruit; (f) pink and red seeded grapefruit...

Discrimination of orange beverage emulsions with different formulations using multivariate analysis.

PubMed

Mirhosseini, Hamed; Tan, Chin Ping

2010-06-01

The constituents in a food emulsion interact with each other, either physically or chemically, determining the overall physico-chemical and organoleptic properties of the final product. Thus, the main objective of present study was to investigate the effect of emulsion components on beverage emulsion properties. In most cases, the second-order polynomial regression models with no significant (P > 0.05) lack of fit and high adjusted coefficient of determination (adjusted R(2), 0.851-0.996) were significantly fitted to explain the beverage emulsion properties as function of main emulsion components. The main effect of gum arabic was found to be significant (P < 0.05) in all response regression models. Orange beverage emulsion containing 222.0 g kg(-1) gum arabic, 2.4 g kg(-1) xanthan gum and 152.7 g kg(-1) orange oil was predicted to provide the desirable emulsion properties. The present study suggests that the concentration of gum arabic should be considered as a primary critical factor for the formulation of orange beverage emulsion. This study also indicated that the interaction effect between xanthan gum and orange oil showed the most significant (P < 0.05) effect among all interaction effects influencing all the physicochemical properties except for density. Copyright (c) 2010 Society of Chemical Industry.

Biochemical characterization of blood orange, sweet orange, lemon, bergamot and bitter orange.

PubMed

Moufida, Saïdani; Marzouk, Brahim

2003-04-01

This paper reports on the composition of aroma compounds and fatty acids and some physico-chemical parameters (juice percentage, acidity and total sugars) in five varieties of citrus: blood orange, sweet orange, lemon, bergamot and bitter orange. Volatile compounds and methyl esters have been analyzed by gas chromatography. Limonene is the most abundant compound of monoterpene hydrocarbons for all of the examined juices. Eighteen fatty acids have been identified in the studied citrus juices, their quantification points out that unsaturated acids predominate over the saturated ones. Mean concentration of fatty acids varies from 311.8 mg/l in blood orange juice to 678 mg/l in bitter orange juice. Copyright 2003 Elsevier Science Ltd.

Late Quaternary environmental history of Lake Valencia, Venezuela

USGS Publications Warehouse

Bradbury, J. Platt; Leyden, B.; Baker, M.R.; Lewis, W.M.; Schubert, C.; Binford, M.W.; Whitehead, D.R.; Weibezahn, F.H.

1981-01-01

Chemical, paleontological, and mineralogical analyses of a 7.5-meter core from the middle of Lake Valencia, Venezuela, have provided information on the paleoclimatic history of this low-elevation, low-latitude site for the last 13,000 years. The data show that dry climates existed in this region from 13,000 years before present (B.P.) until about 10,000 years B.P. The Lake Valencia Basin was occupied by intermittent saline marshes at that time. About 10,000 years B.P., a permanent lake of fluctuating salinity formed and arboreal plant communities replaced the earlier dominant xeric herbaceous vegetation and marsh plants. By 8500 years B.P., Lake Valencia reached moderate to low salinities and discharged water; the modern vegetation became established at that time. After 8500 years B.P., the lake twice ceased discharging as a result of reduced watershed moisture. The second of these drying episodes is still in progress and has been aggravated by human activities in the watershed.

Late quaternary environmental history of Lake Valencia, Venezuela

USGS Publications Warehouse

Platt, Bradbury J.; Leyden, B.; Salgado-Labouriau, M.; Lewis, W.M.; Schubert, C.; Binford, M.W.; Frey, D.G.; Whitehead, D.R.; Weibezahn, F.H.

1981-01-01

Chemical, paleontological, and mineralogical analyses of a 7.5-meter core from the middle of Lake Valencia, Venezuela, have provided information on the paleoclimatic history of this low-elevation, low-latitude site for the last 13,000 years. The data show that dry climates existed in this region from 13,000 years before present (B.P.) until about 10,000 years B.P. The Lake Valencia Basin was occupied by intermittent saline marshes at that time. About 10,000 years B.P., a permanent lake of fluctuating salinity formed and arboreal plant communities replaced the earlier dominant xeric herbaceous vegetation and marsh plants. By 8500 years B.P., Lake Valencia reached moderate to low salinities and discharged water; the modern vegetation became established at that time. After 8500 years B.P., the lake twice ceased discharging as a result of reduced watershed moisture. The second of these drying episodes is still in progress and has been aggravated by human activities in the watershed. Copyright ?? 1981 AAAS.

21 CFR 146.151 - Orange juice for manufacturing.

Code of Federal Regulations, 2011 CFR

2011-04-01

... apply to the hybrid species described in § 146.135). Seeds (except embryonic seeds and small fragments of seeds that cannot be separated by good manufacturing practice) are removed, and pulp and orange oil may be adjusted in accordance with good manufacturing practice. If pulp is added it shall be other...

Larvicidal potential of essential oils against Musca domestica and Anopheles stephensi.

PubMed

Chauhan, Nitin; Malik, Anushree; Sharma, Satyawati; Dhiman, R C

2016-06-01

The larvicidal activity of Mentha piperita, Cymbopogan citratus (lemongrass), Eucalyptus globulus and Citrus sinensis (orange) essential oils and their combinations was evaluated against Musca domestica (housefly) and Anopheles stephensi (mosquitoes) through contact toxicity assay. Among all the tested essential oils/combinations, Me. piperita was found to be the most effective larvicidal agent against Mu. domestica and An. stephensi with LC50 values of 0.66 μl/cm(2) and 44.66 ppm, respectively, after 48 h. The results clearly highlighted that the addition of mentha oil to other oils (1:1 ratio) improved their larvicidal activity. The order of effectiveness of essential oils/combinations indicated that the pattern for An. stephensi follows the trend as mentha > mentha + lemongrass > lemongrass > mentha + eucalyptus > eucalyptus > mentha + orange > orange and for Mu. domestica as mentha > mentha + lemongrass > lemongrass > mentha + orange > orange > mentha + eucalyptus > eucalyptus. The images obtained from scanning electron microscopy (SEM) analysis indicated the toxic effect of Me. piperita as the treated larvae were observed to be dehydrated and deformed. This study demonstrates the effectiveness of tested essential oils/combinations against the larval stages of Mu. domestica and An. stephensi and has the potential for development of botanical formulations.

Effects of rootstock on the composition of bergamot (Citrus bergamia Risso et Poiteau) essential oil.

PubMed

Verzera, Antonella; Trozzi, Alessandra; Gazea, Florea; Cicciarello, Giuseppe; Cotroneo, Antonella

2003-01-01

This paper reports the composition of bergamot oils obtained from plants grafted on the following rootstocks: sour orange, Carrizo citrange, trifoliate orange, Alemow, Volkamerian lemon, and Troyer citrange. The aim of this study is to evaluate the possibility of using rootstocks other than sour orange, checking their effect on the composition of the essential oil. Results are reported for analysis of 203 bergamot oils during the years 1997-1998, 1998-1999, and 1999-2000. The oils were analyzed by HRGC and HRGC/MS; 78 components were identified, and the results were in agreement with those reported in the literature for the Calabrian bergamot oils obtained from industry. Because of the quality of their essential oils, Alemow and Volkamerian lemon can be considered as substitutes for sour orange rootstocks.

Efficacy of three citrus oil formulations against solenopsis invicta buren (Hymenoptera: Formicidae), the red imported fire ant1,2

Treesearch

James T. Vogt; Thormas G. Shelton; Michael E. Merchant; Scott A. Russell; Marla J. Tanley; Arthur G. Appel

2002-01-01

Experiments were conducted in Alabama, Oklahoma, and Texas to assess efficacy of raw citrus peel extract (orange oil) and a commercial citrus oil formulation for control of Solenopsis invicta Buren, the red imported fire ant. A recipe containing orange oil (equal parts orange oil, cattlemen's molasses, and compost tea at 47 mL L1 water),...

Application of an enzyme immunoassay for the quantitative determination of azo dye (Orange II) in food products.

PubMed

Xue, Huyin; Xing, Yue; Yin, Yongmei; Zhang, Taichang; Zhang, Bo; Zhang, Yu; Song, Pei; Tian, Xi; Xu, Yinghui; Wang, Peng; Meng, Meng; Xi, Rimo

2012-01-01

This paper reports the preparation of polyclonal antibodies against a synthetic azo dye, Orange II, and the development of an indirect ELISA to detect Orange II in foods. The sulfonic group of Orange II was modified and linked with carrier protein to synthesise an artificial antigen. Based on the checkerboard titration, the method showed excellent sensitivity (IC₅₀ = 0.61 ng g⁻¹) to Orange II in the linear range of 0.05-10 ng g⁻¹. The antibody had little cross-reactivity with Chromotrope FB, Gardenia Yellow, Ponceau 4R, Sunset Yellow and Sudan dyes. The ELISA had limits of detection (LOD) of 0.22, 0.97 and 0.74 ng g⁻¹ in chilli powder, chilli oil and braised pork, respectively. The limits of quantification (LOQ) of the assay were 0.91 ng g⁻¹ in chilli powder, 1.48 ng g⁻¹ in chilli oil and 1.10 ng g⁻¹ in braised pork. For food products fortified with 1-10 ng g⁻¹ Orange II, the inter- and intra-assay variations were all less than 24.0% and 18.0%, respectively. Therefore, the proposed test could be used as a rapid screening method for Orange II detection in food samples.

Nanoemulsion delivery systems for oil-soluble vitamins: Influence of carrier oil type on lipid digestion and vitamin D3 bioaccessibility.

PubMed

Ozturk, Bengu; Argin, Sanem; Ozilgen, Mustafa; McClements, David Julian

2015-11-15

The influence of carrier oil type on the bioaccessibility of vitamin D3 encapsulated within oil-in-water nanoemulsions prepared using a natural surfactant (quillaja saponin) was studied using a simulated gastrointestinal tract (GIT) model: mouth; stomach; small intestine. The rate of free fatty acid release during lipid digestion decreased in the following order: medium chain triglycerides (MCT) > corn oil ≈ fish oil > orange oil > mineral oil. Conversely, the measured bioaccessibility of vitamin D3 decreased in the following order: corn oil ≈ fish oil > orange oil > mineral oil > MCT. These results show that carrier oil type has a considerable impact on lipid digestion and vitamin bioaccessibility, which was attributed to differences in the release of bioactives from lipid droplets, and their solubilization in mixed micelles. Nanoemulsions prepared using long chain triglycerides (corn or fish oil) were most effective at increasing vitamin bioaccessibility. Copyright © 2015 Elsevier Ltd. All rights reserved.

Comparison of the Composition and Antimicrobial Activities of the Essential Oils of Green Branches and Leaves of Egyptian Navel Orange (Citrus sinensis (L.) Osbeck var. malesy).

PubMed

Eldahshan, Omayma A; Halim, Ahmed F

2016-06-01

The essential oils isolated from the leaves and green branches of the Egyptian navel orange trees were analyzed by GC and GC/MS. A total of 33 and 24 compounds were identified from the oils of the leaves and branches accounting for 96.0% and 97.9%, respectively, of the total detected constituents. The major ones were sabinene (36.5; 33.0%), terpinen-4-ol (8.2; 6.2%), δ-3-carene (7.0; 9.4%), limonene (6.8; 18.7%), trans-ocimene (6.7; 6.1%), and β-myrcene (4.5; 4.4%). The antimicrobial activities of both oils were evaluated using the agar-well diffusion method toward three representatives for each of Gram-positive bacteria, Gram-negative bacteria, and fungi. The oil of leaves was more effective as antimicrobial agent than that of the branches. Streptococcus pyogenes, Staphylococcus aureus, Salmonella typhimurium, and Aspergillus fumigatus were the most sensitive bacteria and fungi by the leaves oil. © 2016 Verlag Helvetica Chimica Acta AG, Zürich.

Evaluating the conservation impact of an innovative zoo-based educational campaign: ‘Don't Palm Us Off’ for orang-utan conservation

PubMed Central

Pearson, Elissa L; Lowry, Rachel; Dorrian, Jillian; Litchfield, Carla A

2014-01-01

With significant biodiversity loss occurring presently, increased emphasis is being placed upon the capacity of zoos to contribute to species conservation. This paper evaluates an innovative conservation education campaign ‘Don't Palm Us Off’ implemented at Melbourne Zoo, Australia. This sought to address a lack of public awareness regarding palm oil (the product most threatening the survival of the orang-utan) and to create public support for mandatory labeling of palm oil on food products, allowing for informed consumer purchasing. Communication tools utilized included an educational video presentation played on-site, as well as You Tube video, celebrity ambassadors, and social media. Evaluation took place across four time-points: baseline, mid-point, conclusion (12 months), and follow-up. Zoo visitors (N = 403) were randomly selected whilst visiting the orang-utan exhibit, completing a questionnaire regarding knowledge about orang-utans, attitudes toward orang-utans, support for palm oil labeling, previous conservation behavior, and intentions for future behavior. Results revealed significant increases in palm oil awareness; attitudes toward orang-utans; support for palm oil labeling; and indicating labeling would influence purchasing behavior, at all times relative to baseline (P < 0.01). There were also significant increases in self-reported conservation behavior at the end of the campaign and follow-up (P < 0.05). In excess of 160,000 people additionally signed an associated petition for mandatory palm oil labeling. Overall the findings support the efficacy of this multi-faceted initiative; highlighting the importance of continued innovation in zoo-based conservation education and practice (including the integration of emerging technologies with traditional on-site education) to maximize contributions to species conservation. Zoo Biol. 33:184–196, 2014. © 2014 The Authors. Zoo Biology published by Wiley Periodicals, Inc. PMID:24535962

Ultra-High Performance Liquid Chromatography (UHPLC) Method for the Determination of Limonene in Sweet Orange (Citrus sinensis) Oil: Implications for Limonene Stability.

PubMed

Bernart, Matthew W

2015-01-01

The citrus-derived bioactive monoterpene limonene is an important industrial commodity and fragrance constituent. An RP isocratic elution C18 ultra-HPLC (UHPLC) method using a superficially porous stationary phase and photodiode array (PDA) detector has been developed for determining the limonene content of sweet orange (Citrus sinensis) oil. The method is fast with a cycle time of 1.2 min, linear, precise, accurate, specific, and stability indicating, and it satisfies U.S. Pharmacopeia suitability parameters. The method may be useful in its present form for limonene processing, or modified for research on more polar compounds of the terpenome. A forced-degradation experiment showed that limonene is degraded by heat in hydro-ethanolic solution. PDA detection facilitates classification of minor components of the essential oil, including β-myrcene.

Dualities in Architectural Training: The Architecture School of Valencia (1968-1975)

ERIC Educational Resources Information Center

Domingo-Calabuig, Débora

2018-01-01

The school of Valencia was a singular case study in the architectural training in Spain towards the end of the 60s. Like in Madrid, Barcelona, and Seville, the school also participated in the bustling political and social context, but while in these schools the curriculum of 1964 was extended until 1975, in Valencia the creation of the Instituto…

A photoswitchable orange-to-far-red fluorescent protein, PSmOrange.

PubMed

Subach, Oksana M; Patterson, George H; Ting, Li-Min; Wang, Yarong; Condeelis, John S; Verkhusha, Vladislav V

2011-07-31

We report a photoswitchable monomeric Orange (PSmOrange) protein that is initially orange (excitation, 548 nm; emission, 565 nm) but becomes far-red (excitation, 636 nm; emission, 662 nm) after irradiation with blue-green light. Compared to its parental orange proteins, PSmOrange has greater brightness, faster maturation, higher photoconversion contrast and better photostability. The red-shifted spectra of both forms of PSmOrange enable its simultaneous use with cyan-to-green photoswitchable proteins to study four intracellular populations. Photoconverted PSmOrange has, to our knowledge, the most far-red excitation peak of all GFP-like fluorescent proteins, provides diffraction-limited and super-resolution imaging in the far-red light range, is optimally excited with common red lasers, and can be photoconverted subcutaneously in a mouse. PSmOrange photoswitching occurs via a two-step photo-oxidation process, which causes cleavage of the polypeptide backbone. The far-red fluorescence of photoconverted PSmOrange results from a new chromophore containing N-acylimine with a co-planar carbon-oxygen double bond.

Detection of oranges from a color image of an orange tree

NASA Astrophysics Data System (ADS)

Weeks, Arthur R.; Gallagher, A.; Eriksson, J.

1999-10-01

The progress of robotic and machine vision technology has increased the demand for sophisticated methods for performing automatic harvesting of fruit. The harvesting of fruit, until recently, has been performed manually and is quite labor intensive. An automatic robot harvesting system that uses machine vision to locate and extract the fruit would free the agricultural industry from the ups and downs of the labor market. The environment in which robotic fruit harvesters must work presents many challenges due to the inherent variability from one location to the next. This paper takes a step towards this goal by outlining a machine vision algorithm that detects and accurately locates oranges from a color image of an orange tree. Previous work in this area has focused on differentiating the orange regions from the rest of the picture and not locating the actual oranges themselves. Failure to locate the oranges, however, leads to a reduced number of successful pick attempts. This paper presents a new approach for orange region segmentation in which the circumference of the individual oranges as well as partially occluded oranges are located. Accurately defining the circumference of each orange allows a robotic harvester to cut the stem of the orange by either scanning the top of the orange with a laser or by directing a robotic arm towards the stem to automatically cut it. A modified version of the K- means algorithm is used to initially segment the oranges from the canopy of the orange tree. Morphological processing is then used to locate occluded oranges and an iterative circle finding algorithm is used to define the circumference of the segmented oranges.

school in Valencia before the Civil War.

PubMed

Ferrandis, Xavier García; Vidal, Àlvar Martínez

During the first third of the 20th century, the dental profession in Spain was disputed by several groups of healthcare professionals, including surgery practitioners and dental technicians. The most intense conflict was between dentists and stomatologists. In the case of Valencia, this struggle became apparent in the attempt to create a dental school during the first Spanish Republican period. This project was supported by the Faculty of Medicine and by the local authorities but was not implemented due to the special interests of practicing dentists and the School of Dentistry in the Central University of Madrid. The institutionalization of dentistry in Valencia was limited to the teaching of an isolated subject in the Faculty of Medicine, preventing the adoption of a university model that would have encouraged the consolidation of the dental profession in all its aspects, not solely in terms of improvements in dental care. Opposition to the project was largely based on a campaign to discredit its main promoter, Rafael Fiol Vilar (1885-1971). This Valencian doctor and dentist is virtually unknown by historians, but he had an excellent academic background and an extraordinary international projection for that time. He may have been ignored because of his exile after the Spanish Civil War and the failure of his project for the University of Valencia.

Uniformity of plants regenerated from orange (Citrus sinensis Osb.) protoplasts.

PubMed

Kobayashi, S

1987-05-01

Using 25 plants (protoclones) regenerated from orange (Citrus sinensis Osb.) protoplasts, several characters, including leaf and flower morphology, leaf oil, isozyme patterns and chromosome number, were examined. No significant variations in each character were recorded among the protoclones. Uniformity observed among protoclones was identical to that of nucellar seedlings.

[R-ALERGO. Allergy-healthy routes in Valencia].

PubMed

Temes Cordovez, Rafael R; Moya Fuero, Alfonso; Martí Garrido, Jaume; Perales Chordá, Carolina; Díaz Palacios, Miguel; Hernández Fernández de Rojas, Dolores

2016-01-01

R-ALERGO is a project developed by researchers from the Universitat Politècnica de València and the Hospital Universitario La Fe (Valencia, Spain). The main objective of the project is to create a mobile application identifying, within the city of Valencia, the most favorable routes for allergic individuals. The application is developed using nine environmental variables with a potential effect on the development of clinical manifestations in allergic individuals. The application combines the use of spatial analysis based on network technology and implemented with a geographic information system software. The first 01 version is under evaluation for a Healthy app hallmark. The next step in this project is to design a clinical validation process to test its usefulness in allergic individuals. Copyright © 2015 SESPAS. Published by Elsevier Espana. All rights reserved.

Life cycle toxicity assessment of pesticides used in integrated and organic production of oranges in the Comunidad Valenciana, Spain.

PubMed

Juraske, Ronnie; Sanjuán, Neus

2011-02-01

The relative impacts of 25 pesticides including acaricides, fungicides, herbicides, insecticides, and post-harvest fungicides, used in the production of oranges in Spain were assessed with current life cycle impact assessment (LCIA) tools. Chemical specific concentrations were combined with pesticide emission data and information on chemical toxicity to assess human toxicity and freshwater ecotoxicity impacts. As a case study, the relative impacts of two orange production systems in the region of Valencia, integrated pest management (IP) and organic production (OP), were assessed. The evaluation of active ingredients showed that on average acaricides have the highest human toxicity impact scores, while for freshwater ecotoxicity insecticides show the highest impact. In both impact categories the lowest impact scores were calculated for herbicides. In the production of 1 kg of orange fruits, where several kinds of pesticides are combined, results show that post-harvest fungicides can contribute more than 95% to the aggregate human toxicity impacts. More than 85% of aquatic ecotoxicity is generated by fungicides applied before harvest. The potential to reduce impacts on freshwater ecosystems is seven orders of magnitude, while impacts on human health can be reduced by two orders of magnitude. Hence, this stresses the importance of a careful pre-selection of active ingredients. In both impact categories, organic production represents the least toxic pest-control method. Copyright © 2010 Elsevier Ltd. All rights reserved.

Application of tristimulus colorimetry to estimate the carotenoids content in ultrafrozen orange juices.

PubMed

Meléndez-Martínez, Antonio J; Vicario, Isabel M; Heredia, Francisco J

2003-12-03

Tristimulus Colorimetry was applied to characterize the color of Valencia late orange juices. Color measurements were made against white background and black background. The profile of the main carotenoids related to the color of the juices was determined by HPLC. Significant correlations (p < 0.05) between b*, Cab* and h(ab) and the content of beta-cryptoxanthin, lutein + zeaxanthin and beta-carotene were found. The correlations between the color parameters L*, a*, b*, Cab* and h(ab) and the carotenoids content were also explored by partial least squares. The results obtained have shown that it is possible to obtain equations, by means of multiple regression models, which allow the determination of the individual carotenoid levels from the CIELAB color parameters, with R2 values always over 0.9. In this sense, equations have been proposed to calculate the retinol equivalents (1 RE = 1 microgram retinol = 12 micrograms beta-carotene = 24 micrograms alpha-carotene = 24 micrograms beta-cryptoxanthin) of the orange juice analyzed as a function of the color parameters calculated from measurement made against white and black backgrounds. The average RE per liter of juice obtained by HPLC was 51.07 +/- 18.89, whereas employing these equations, average RE values obtained were 51.16 +/- 1.36 and 51.21 +/- 1.70 for white background and black background, respectively.

Occurrence of methylmercury in Lake Valencia, Venezuela

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jaffe, R.; Cai, Y.; West-Thomas, J.

1997-12-31

The presence of mercury in the environment has received renewed attention during recent years. This is in part due to the known human health and ecological effects of the highly toxic organomercury compounds, and to the fact that novel and improved analytical techniques such as atomic fluorescence spectroscopy (AFS) and capillary chromatography with AFS detection, have enhanced significantly the detection of trace amounts of mercury and organo mercurials in environmental samples. Such techniques have allowed for a better understanding of the biogeochemical cycle of mercury in the aquatic environment. This paper reports on the presence of methylmercury in the watermore » column and sediments of a hyper-eutrophic lake. Lake Valencia is a freshwater lake located in North-Central Venezuela`s Aragua Valley. The lake`s surface area covers approximately 350 km{sup 2}, with a mean depth of 19 m and a maximum depth of 41 m. Due to the discharge of waste waters from the cities of Maracay and Valencia, as well as from other smaller villages and agricultural areas in its watershed, Lake Valencia has become hyper-eutrophic. The population of phytoplankton, particularly of blue-green algae, has increased dramatically during the last two decades resulting in anoxic conditions in the lower part of the water column during most of the year. In addition, concentrations of anthropogenic chemicals, including heavy metals, have increased in the Lake during the last four decades. 15 refs., 2 figs.« less

Biology of the Huanglongbing vector Diaphorina citri (Hemiptera: Liviidae) on different host plants.

PubMed

Alves, G R; Diniz, A J F; Parra, J R P

2014-04-01

Although many studies have been conducted on the development and reproductive potential of Diaphorina citri Kuwayama, 1908 (Hemiptera: Liviidae) in different host species, few have evaluated these parameters on different varieties of the same host species. This study evaluated the influence of five commercial varieties of citrus (Citrus spp. L.)--Hamlin, Natal, Pêra, Ponkan, and Valencia-and orange jasmine [Murraya exotica (L.) Jack] on the development of D. citri. Survival rates for the egg stage were highest on orange jasmine (85.7%) and on Valencia (83.3%). The lowest viability of the nymphal stage was also observed on Hamlin, averaging 57.4%. Values for total viability ranged from 65.9 to 32.6%, and were highest on Valencia. The longest egg-adult development time was on Natal, with a mean of 18.4 d; the shortest total development time was on orange jasmine, with a mean of 17.3 d. Based on the fertility life table, the net reproductive rate (Ro) of D. citri was 2.5 times higher when reared on Valencia than on Hamlin. The other parameters (duration of each generation [T], finite rate of increase [lambda], and innate capacity to increase in number [r(m)]) also demonstrated that Valencia is best suited to this insect. The results obtained for the biological parameters and the fertility life table indicate that Valencia and orange jasmine were the most suitable hosts, whereas Hamlin was least suitable for the development of D. citri. These results provide information for the installation of new citrus groves, especially in the choice of varieties to be planted and the location of different varieties within the groves, with a view toward the management of Huanglongbing or HLB.

Tertiary sedimentary history and structure of the Valencia trough (western Mediterranean)

NASA Astrophysics Data System (ADS)

Maillard, A.; Mauffret, A.; Watts, A. B.; Torné, M.; Pascal, G.; Buhl, P.; Pinet, B.

1992-03-01

We present here main results of the Common Depth Point (CDP) data acquired during the Valsis 2 Cruise in 1988 in the Valencia trough. The profiles are tied in with industrial well data and this correlation allows the sedimentary and structural history of the region to be deduced. The Valsis Cruise seismic profiles have been supplemented by a very dense grid of industrial seismic lines and these data permit us to establish an accurate depth to basement map. The formation of the initial grabens, coeval with those of the Gulf of Lions, is related to the Early Miocene opening of the northwestern Mediterranean basin and the Barcelona graben is filled by the same sedimentary layers, including evaporites, as that of the Provençal region. Nevertheless, the Valencia-Catalan grabens have been reactivated by young extensional tectonics which could be a consequence of the convergence of Africa relative to Europe. The Valencia trough is segmented by transfer faults which trend NW-SE. These faults, which have a more accentuated structural expression than the Valencia and Catalonia grabens, may act as transform faults separating the individual Balearic Islands. The transfer faults are in strike with volcanic ridges which have been sampled during the DSDP Leg 13. The dense seismic grid allows us to delineate several widespread volcanic features in the Valencia trough which have been active from the Early Miocene to the Pleistocene. However, we note that the volcanic features are mainly Miocene in age whereas the recent volcanism is restricted to a narrow zone (Columbretes Islands). The compressional tectonics which deformed the Balearic Islands does not appear to extend far towards the North. We delineate the compressional front north of Ibiza, but we failed to determine any thrust or fold north of Mallorca, whereas an extensional tectonics is evident.

Citrus essential oils and four enantiomeric pinenes against Culex pipiens (Diptera: Culicidae).

PubMed

Michaelakis, Antonios; Papachristos, Dimitrios; Kimbaris, Athanasios; Koliopoulos, George; Giatropoulos, Athanasios; Polissiou, Moschos G

2009-09-01

The aim of this study was to evaluate the toxicity of pinenes (enantiomers of alpha- and beta-) and essential oils from Greek plants of the Rutaceae family against the mosquito larvae of Culex pipiens (Diptera: Culicidae). Essential oils were isolated by hydrodistillation from fruit peel of orange (Citrus sinensis L.), lemon (Citrus limon L.), and bitter orange (Citrus aurantium L.). The chemical composition was determined by gas chromatography/mass spectrometry (GC/MS) analysis. Citrus essential oils contained in high proportion limonene and in lower quantities p-menthane molecules and pinenes. The insecticidal action of these essential oils and enantiomers of their pinenes on mosquito larvae was evaluated. Plant essential oils exhibited strong toxicity against larvae with the LC(50) values ranging from 30.1 (lemon) to 51.5 mg/L (orange) depending on Citrus species and their composition. Finally, the LC(50) value of pinenes ranging from 36.53 to 66.52 mg/L indicated an enantioselective toxicity only for the beta-pinene enantiomer.

Fortification of orange juice with vitamin D: a novel approach for enhancing vitamin D nutritional health.

PubMed

Tangpricha, Vin; Koutkia, Polyxeni; Rieke, Suzanne M; Chen, Tai C; Perez, Alberto A; Holick, Michael F

2003-06-01

Fortification of milk with vitamin D may not be adequate for satisfying the vitamin D requirement because of variability in vitamin D content after fortification and because many persons have milk allergy or lactose intolerance. Additional foods need to be fortified with vitamin D. We determined whether vitamin D, a fat-soluble vitamin, is bioavailable in orange juice and skim milk, 2 nonfat beverages. On 3 separate occasions, 18 adults ingested 25 000 IU vitamin D(2) in 240 mL whole milk or skim milk or in 0.1 mL corn oil applied to toast. A separate, double-blind, randomized, controlled trial investigated whether the consumption of orange juice fortified with vitamin D(3) would increase serum 25-hydroxyvitamin D [25(OH)D] concentrations: 14 subjects ingested 240 mL orange juice fortified with 1000 IU vitamin D, and 12 subjects ingested a control orange juice daily for 12 wk. Peak serum vitamin D(2) concentrations did not differ significantly after the ingestion of vitamin D(2) in whole milk, skim milk, or corn oil on toast. After subjects consumed orange juice fortified with 1000 IU vitamin D(3) daily for 12 wk, serum 25(OH)D(3) concentrations increased by 150%, and serum parathyroid hormone concentrations decreased by 25% compared with baseline; control subjects had a seasonal increase of 45% in 25(OH)D and no significant change in serum parathyroid hormone. The fat content of milk does not affect vitamin D bioavailability. Vitamin D fortification at 1000 IU/240 mL orange juice for 12 wk safely increased 25(OH)D(3) concentrations in adults.

The survival of three strains of Arcobacter butzleri in the presence of lemon, orange and bergamot essential oils and their components in vitro and on food.

PubMed

Fisher, K; Rowe, C; Phillips, C A

2007-05-01

To test the effect of oils and vapours of lemon, sweet orange and bergamot and their components against three Arcobacter butzleri strains. The disc diffusion method was used to screen the oils and vapours against three strains of A. butzleri. In vitro bergamot was the most inhibitory essential oil (EO) and both citral and linalool were effective. On cabbage leaf, the water isolate was the least susceptible to bergamot EO, citral and linalool (1-2 log reduction), with the chicken isolate being the most susceptible (6-8 log reduction). However, the latter appeared not to be susceptible to vapours over 24 h although type strain and water isolate populations reduced by 8 logs. On chicken skin, the effectiveness of the oils was reduced compared with that on cabbage leaf. Bergamot was the most effective of the oils tested and linalool the most effective component. All strains tested were less susceptible in food systems than in vitro. Arcobacter isolates vary in their response to EO suggesting that the results of type strain studies should be interpreted with caution. Bergamot EO has the potential for the inhibition of this 'emerging' pathogen.

7 CFR 906.13 - Variety or varieties.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Variety or varieties. 906.13 Section 906.13 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing...) Navel oranges; (b) Early and Midseason oranges, except Navel oranges; (c) Valencia and similar late type...

7 CFR 457.121 - Arizona-California citrus crop insurance provisions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... & Sweet oranges 38 Container #58 Lemons 40 Container #59 Grapefruit 32 Container #63 Tangerines (including... California lemons; (ii) November 20 for Valencia oranges; and (iii) July 31 for all other citrus crops. (b...

7 CFR 457.121 - Arizona-California citrus crop insurance provisions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... & Sweet oranges 38 Container #58 Lemons 40 Container #59 Grapefruit 32 Container #63 Tangerines (including... California lemons; (ii) November 20 for Valencia oranges; and (iii) July 31 for all other citrus crops. (b...

7 CFR 457.121 - Arizona-California citrus crop insurance provisions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... & Sweet oranges 38 Container #58 Lemons 40 Container #59 Grapefruit 32 Container #63 Tangerines (including... California lemons; (ii) November 20 for Valencia oranges; and (iii) July 31 for all other citrus crops. (b...

Work, rice and malaria in Valencia in the XVIIIth century.

PubMed

Riera Palmero, J

1994-01-01

This study, prepared with documentary sources from archives, focuses on one of the most serious health problems in Spain in the age of Enlightenment; that is, the pernicious endemics of malaria in the humid Mediterranean zones. Based on material from the General Archives of Simancas, the author studies the health polemic between the partisans and detractors of rice cultivation in the swampy bogs of the Turia and Júcar river areas. To these considerations on Valencia's agricultural expansion in the 18th century is added a documented study on malaria in the ancient kingdom of Valencia, a theme which underlies this historiographic contribution.

NuMex-02- A High oleic Valencia peanut with partial resistance to Sclerotinia blight

USDA-ARS?s Scientific Manuscript database

NuMex-02 is a high oleic Valencia peanut (Arachis hypogaea L., subsp. fastigiata var. fastigiata) cultivar developed by the New Mexico Agricultural Experiment Station, Clovis, New Mexico. NuMex-02 originated from a cross made between NM Valencia A and OLin. Pedigree selection was practiced based on...

7 CFR 905.5 - Variety.

Code of Federal Regulations, 2010 CFR

2010-01-01

... of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and... Gong, and similar late maturing oranges of the Valencia type; (b) Valencia, Lue Gim Gong, and similar... seedless grapefruit; (g) Pink seeded grapefruit; (h) Tangelos; (i) Dancy and similar tangerines, excluding...

The monoterpene limonene in orange peels attracts pests and microorganisms

PubMed Central

Rodríguez, Ana; Andrés, Victoria San; Cervera, Magdalena; Redondo, Ana; Alquézar, Berta; Shimada, Takehiko; Gadea, José; Rodrigo, María; Zacarías, Lorenzo; Palou, Lluís; López, María M.; Castañera, Pedro; Peña, Leandro

2011-01-01

Plant volatiles include terpenoids, which are generally involved in plant defense, repelling pests and pathogens and attracting insects for herbivore control, pollination and seed dispersal. Orange fruits accumulate the monoterpene limonene at high levels in the oil glands of their fruit peels. When limonene production was downregulated in orange fruits by the transgenic expression of a limonene synthase (CitMTSE1) in the antisense configuration, these fruits were resistant to the fungus Penicillium digitatum (Pers.) Sacc. and the bacterium Xanthomonas citri subsp. citri and were less attractive to the medfly pest Ceratitis capitata. These responses were reversed when the antisense transgenic orange fruits were treated with limonene. To gain more insight into the role of the limonene concentration in fruit responses to pests and pathogens, we attempted to overexpress CitMTSE1 in the sense configuration in transgenic orange fruits. Only slight increases in the amount of limonene were found in sense transgenic fruits, maybe due to the detrimental effect that excessive limonene accumulation would have on plant development. Collectively, these results suggest that when limonene reaches peak levels as the fruit develops, it becomes a signal for pest and pathogen attraction, which facilitate access to the fruit for pulp consumers and seed dispersers. PMID:22212123

Food matrix effects on in vitro digestion of microencapsulated tuna oil powder.

PubMed

Shen, Zhiping; Apriani, Christina; Weerakkody, Rangika; Sanguansri, Luz; Augustin, Mary Ann

2011-08-10

Tuna oil, containing 53 mg of eicosapentaenoic acid (EPA) and 241 mg of docosahexaenoic acid (DHA) per gram of oil, delivered as a neat microencapsulated tuna oil powder (25% oil loading) or in food matrices (orange juice, yogurt, or cereal bar) fortified with microencapsulated tuna oil powder was digested in simulated gastric fluid or sequentially in simulated gastric fluid and simulated intestinal fluid. The level of fortification was equivalent to 1 g of tuna oil per recommended serving size (i.e., per 200 g of orange juice or yogurt or 60 g of cereal bar). The changes in particle size of oil droplets during digestion were influenced by the method of delivery of the microencapsulated tuna oil powder. Lipolysis in simulated gastric fluid was low, with only 4.4-6.1% EPA and ≤1.5% DHA released after digestion (as a % of total fatty acids present). After sequential exposure to simulated gastric and intestinal fluids, much higher extents of lipolysis of both glycerol-bound EPA and DHA were obtained (73.2-78.6% for the neat powder, fortified orange juice, and yogurt; 60.3-64.0% for the fortified cereal bar). This research demonstrates that the choice of food matrix may influence the lipolysis of microencapsulated tuna oil.

Changes of Peel Essential Oil Composition of Four Tunisian Citrus during Fruit Maturation

PubMed Central

Bourgou, Soumaya; Rahali, Fatma Zohra; Ourghemmi, Iness; Saïdani Tounsi, Moufida

2012-01-01

The present work investigates the effect of ripening stage on the chemical composition of essential oil extracted from peel of four citrus: bitter orange (Citrus aurantium), lemon (Citrus limon), orange maltaise (Citrus sinensis), and mandarin (Citrus reticulate) and on their antibacterial activity. Essential oils yields varied during ripening from 0.46 to 2.70%, where mandarin was found to be the richest. Forty volatile compounds were identified. Limonene (67.90–90.95%) and 1,8-cineole (tr-14.72%) were the most represented compounds in bitter orange oil while limonene (37.63–69.71%), β-pinene (0.63–31.49%), γ-terpinene (0.04–9.96%), and p-cymene (0.23–9.84%) were the highest ones in lemon. In the case of mandarin, the predominant compounds were limonene (51.81–69.00%), 1,8-cineole (0.01–26.43%), and γ-terpinene (2.53–14.06%). However, results showed that orange peel oil was dominated mainly by limonene (81.52–86.43%) during ripening. The results showed that ripening stage influenced significantly the antibacterial activity of the oils against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. This knowledge could help establish the optimum harvest date ensuring the maximum essential oil, limonene, as well as antibacterial compounds yields of citrus. PMID:22645427

Safety and Feasibility of Topical Application of Limonene as a Massage Oil to the Breast.

PubMed

Miller, Jessica A; Thompson, Patricia A; Hakim, Iman A; Lopez, Ana Maria; Thomson, Cynthia A; Chew, Wade; Hsu, Chiu-Hsieh; Chow, H-H Sherry

2012-10-01

Limonene, a major component in citrus oil, has demonstrated anti-cancer effects in preclinical mammary cancer models. However, the effective oral dose translates to a human dose that may not be feasible for chronic dosing. We proposed to evaluate topical application of limonene to the breast as an alternative dosing strategy. We conducted a mouse disposition study to determine whether limonene would be bio available in the mammary tissue after topical application. SKH-1 mice received topical or oral administration of limonene in the form of orange oil every day for 4 weeks. Plasma and mammary pads were collected 4 hrs after the final dosing. We also conducted an exploratory clinical study to evaluate the safety and feasibility of topically applied limonene in the form of orange oil to the breast. Healthy women were recruited to apply orange oil containing massage oil to their breasts daily for four weeks. Safety and feasibility were assessed by reported adverse events, clinical labs, and usage compliance. Pre and post-intervention nipple aspirate fluid (NAF) and plasma were collected for limonene concentration determination. The mouse disposition study showed that topical and oral orange oil administration resulted in similar mammary tissue disposition of limonene with no clinical signs of toxicity. In the clinical study, the topical application of limonene containing massage oil to the breast was found to be safe with high levels of usage compliance for daily application, although NAF and plasma limonene concentrations were not significantly changed after the massage oil application. Our studies showed that limonene is bio available in mammary tissue after topical orange oil application in mice and this novel route of administration to the breast is safe and feasible in healthy women.

Safety and Feasibility of Topical Application of Limonene as a Massage Oil to the Breast

PubMed Central

Miller, Jessica A.; Thompson, Patricia A.; Hakim, Iman A.; Lopez, Ana Maria; Thomson, Cynthia A.; Chew, Wade; Hsu, Chiu-Hsieh; Chow, H.-H. Sherry

2013-01-01

Background Limonene, a major component in citrus oil, has demonstrated anti-cancer effects in preclinical mammary cancer models. However, the effective oral dose translates to a human dose that may not be feasible for chronic dosing. We proposed to evaluate topical application of limonene to the breast as an alternative dosing strategy. Materials and Methods We conducted a mouse disposition study to determine whether limonene would be bio available in the mammary tissue after topical application. SKH-1 mice received topical or oral administration of limonene in the form of orange oil every day for 4 weeks. Plasma and mammary pads were collected 4 hrs after the final dosing. We also conducted an exploratory clinical study to evaluate the safety and feasibility of topically applied limonene in the form of orange oil to the breast. Healthy women were recruited to apply orange oil containing massage oil to their breasts daily for four weeks. Safety and feasibility were assessed by reported adverse events, clinical labs, and usage compliance. Pre and post-intervention nipple aspirate fluid (NAF) and plasma were collected for limonene concentration determination. Results The mouse disposition study showed that topical and oral orange oil administration resulted in similar mammary tissue disposition of limonene with no clinical signs of toxicity. In the clinical study, the topical application of limonene containing massage oil to the breast was found to be safe with high levels of usage compliance for daily application, although NAF and plasma limonene concentrations were not significantly changed after the massage oil application. Conclusions Our studies showed that limonene is bio available in mammary tissue after topical orange oil application in mice and this novel route of administration to the breast is safe and feasible in healthy women. PMID:24236248

A comparison of processed and fresh squeezed ‘Hamlin’ orange juice - flavor quality

USDA-ARS?s Scientific Manuscript database

‘Hamlin’ orange juice was extracted by a commercial food service juicer with or without pasteurization and compared to processed juice for flavor quality. There was much higher peel oil content (introduced from the flavedo), but lower insoluble solids and pectin content (introduced from albedo and s...

Activity of citrus essential oils against Escherichia coli O157:H7 and Salmonella spp. and effects on beef subprimal cuts under refrigeration.

PubMed

Pittman, Curtis I; Pendleton, Sean; Bisha, Bledar; O'Bryan, Corliss A; Belk, Keith E; Goodridge, Lawrence; Crandall, Philip G; Ricke, Steven C

2011-08-01

Escherichia coli O157:H7 and Salmonella spp. are bacterial pathogens often associated with beef, and cause many cases of foodborne illness each year in the United States. During beef slaughter and processing, these bacteria may spread from the hide or intestines to the carcass. The objective of this research was to investigate the use of naturally occurring compounds citrus essential oils (CEOs) extracted from orange peel to reduce or eliminate these pathogens at the chilling stage of processing, or during fabrication. Brisket flats (used to simulate beef subprimals) were spot inoculated with approximately 6 log of surrogate generic E. coli cocktail (previously shown to be identical in growth and survival parameters to E. coli O157:H7 and Salmonella spp.). Following drying, CEOs were applied by spraying at concentrations of 3% and 6% to the surface of different pieces of meat. Treatments were applied using a custom built spray cabinet at 2.07 bar and applied at a rate of 3.79 L/min to replicate commercial practices. The CEOs significantly reduced (P < 0.05) the concentration of E. coli on the brisket flats in comparison to inoculated no spray or water sprayed controls over a period of 90 d, while causing an initial reduction of approximately 1.4 log units. Total aerobic bacteria and psychrotrophic counts were also reduced on uninoculated briskets following treatment. These results indicate that 3% cold-pressed terpeneless Valencia orange oil could be used as an additional intervention against E. coli O157:H7 and Salmonella spp. at the refrigerated storage stage of processing. CEOs are natural compounds that have been designated as Generally Recognized as Safe (GRAS). They can be used to control Salmonella spp. and E. coli O157:H7 on beef carcasses at the chilling stage. © 2011 Institute of Food Technologists®

Profiling of bioactive compounds in cultivars of Runner and Valencia peanut market-types using liquid chromatography/APCI mass spectrometry.

PubMed

Chukwumah, Yvonne; Walker, Lloyd; Vogler, Bernhard; Verghese, Martha

2012-05-01

Peanuts are classified into four market-types (Runners, Spanish, Virginia and Valencia). Studies on their phytochemical composition have focused mainly on market-types other than Valencia. The objectives of this study are to evaluate the phytochemical composition of cultivars of Valencia and Runner market-types. Extracts of 25 peanut cultivars of Runner and Valencia market-types were analysed using HPLC-DAD-MS analysis. Results showed major differences in UV profile of the market-types. A major peak with m/z 317 identified as isorhamnetin was present only in Valencia cultivars while its glycoside (isorhamnetin-3-O-rutinoside) having m/z 625 was identified in both market-types. Genistein, daidzein, rutin, quercetin and trans-resveratrol were also identified and quantified. Genistein and daidzein concentrations (0.03mg/100g) were similar in both market-types. trans-Resveratrol and rutin were significantly (p<0.05) higher in Runner cultivars while quercetin was 10-fold higher (0.60±0.04mg/100g) in Valencia cultivars making them a better source of this phytochemical. Copyright © 2011 Elsevier Ltd. All rights reserved.

Optimization of ultrasonic emulsification conditions for the production of orange peel essential oil nanoemulsions.

PubMed

Hashtjin, Adel Mirmajidi; Abbasi, Soleiman

2015-05-01

The aim of the present study was to investigate the influence of emulsifying conditions on some physical and rheological properties of orange peel essential oil (OPEO) in water nanoemulsions. In this regard, using the response surface methodology, the influence of ultrasonication conditions including sonication amplitude (70-100 %), sonication time (90-150 s) and process temperature (5-45 °C) on the mean droplets diameter (Z-average value), polydispersity index (PDI), and viscosity of the OPEO nanoemulsions was evaluated. In addition, the flow behavior and stability of selected nanoemulsions was evaluated during storage (up to 3 months) at different temperatures (5, 25 and 45 °C). Based on the results of the optimization, the optimum conditions for producing OPEO nanoemulsions (Z-average value 18.16 nm) were determined as 94 % (sonication amplitude), 138 s (sonication time) and 37 °C (process temperature). Moreover, analysis of variance (ANOVA) showed high coefficients of determination values (R (2) > 0.95) for the response surface models of the energy input and Z-average. In addition, the flow behavior of produced nanoemulsions was Newtonian, and the effect of time and storage temperature as well as their interactions on the Z-average value was highly significant (P < 0.0001).

Valencia Community College Educational Technology Plan, 2000-2004.

ERIC Educational Resources Information Center

Valencia Community Coll., Orlando, FL.

The purpose of the Educational Technology Plan is to provide a collaborative framework to address the institutional assessment, prioritization, implementation, and resource allocation associated with technology issues at Valencia Community College (Florida). The plan has been integrated with the college's Comprehensive Strategic Plan goals and…

NASA AVIRIS Aids in Gulf Oil Spill Response

NASA Image and Video Library

2010-05-27

This image from NASA Airborne Visible/Infrared Imaging Spectrometer instrument AVIRIS was collected on May 17, 2010, over the site of the Deepwater Horizon BP oil spill disaster. In the image, crude oil on the surface appears orange to brown.

Accumulation of Carotenoids and Expression of Carotenoid Biosynthetic Genes during Maturation in Citrus Fruit1

PubMed Central

Kato, Masaya; Ikoma, Yoshinori; Matsumoto, Hikaru; Sugiura, Minoru; Hyodo, Hiroshi; Yano, Masamichi

2004-01-01

The relationship between carotenoid accumulation and the expression of carotenoid biosynthetic genes during fruit maturation was investigated in three citrus varieties, Satsuma mandarin (Citrus unshiu Marc.), Valencia orange (Citrus sinensis Osbeck), and Lisbon lemon (Citrus limon Burm.f.). We cloned the cDNAs for phytoene synthase (CitPSY), phytoene desaturase (CitPDS), ζ-carotene (car) desaturase (CitZDS), carotenoid isomerase (CitCRTISO), lycopene β-cyclase (CitLCYb), β-ring hydroxylase (CitHYb), zeaxanthin (zea) epoxidase (CitZEP), and lycopene ε-cyclase (CitLCYe) from Satsuma mandarin, which shared high identities in nucleotide sequences with Valencia orange, Lisbon lemon, and other plant species. With the transition of peel color from green to orange, the change from β,ε-carotenoid (α-car and lutein) accumulation to β,β-carotenoid (β-car, β-cryptoxanthin, zea, and violaxanthin) accumulation was observed in the flavedos of Satsuma mandarin and Valencia orange, accompanying the disappearance of CitLCYe transcripts and the increase in CitLCYb transcripts. Even in green fruit, high levels of β,ε-carotenoids and CitLCYe transcripts were not observed in the juice sacs. As fruit maturation progressed in Satsuma mandarin and Valencia orange, a simultaneous increase in the expression of genes (CitPSY, CitPDS, CitZDS, CitLCYb, CitHYb, and CitZEP) led to massive β,β-xanthophyll (β-cryptoxanthin, zea, and violaxanthin) accumulation in both the flavedo and juice sacs. The gene expression of CitCRTISO was kept low or decreased in the flavedo during massive β,β-xanthophyll accumulation. In the flavedo of Lisbon lemon and Satsuma mandarin, massive accumulation of phytoene was observed with a decrease in the transcript level for CitPDS. Thus, the carotenoid accumulation during citrus fruit maturation was highly regulated by the coordination of the expression among carotenoid biosynthetic genes. In this paper, the mechanism leading to diversity in �

GUS expression in sweet oranges (Citrus sinensis L. Osbeck) driven by three different phloem-specific promoters.

PubMed

Miyata, Luzia Yuriko; Harakava, Ricardo; Stipp, Liliane Cristina Libório; Mendes, Beatriz Madalena Januzzi; Appezzato-da-Glória, Beatriz; de Assis Alves Mourão Filho, Francisco

2012-11-01

Huanglongbing (HLB) is associated with Candidatus Liberibacter spp., endogenous, sieve tube-restricted bacteria that are transmitted by citrus psyllid insect vectors. Transgenic expression in the phloem of specific genes that might affect Ca. Liberibacter spp. growth and development may be an adequate strategy to improve citrus resistance to HLB. To study specific phloem gene expression in citrus, we developed three different binary vector constructs with expression cassettes bearing the β-glucuronidase (GUS) reporter gene (uidA) under the control of one of the three different promoters: Citrus phloem protein 2 (CsPP2), Arabidopsis thaliana phloem protein 2 (AtPP2), and Arabidopsis thaliana sucrose transporter 2 (AtSUC2). Transgenic lines of 'Hamlin', 'Pera', and 'Valencia' sweet oranges [Citrus sinensis (L.) Osbeck] were produced via Agrobacterium tumefaciens transformation. The epicotyl segments collected from in vitro germinated seedlings were used as explants. The gene nptII, which confers resistance to the antibiotic kanamycin, was used for selection. The transformation efficiency was expressed as the number of GUS-positive shoots over the total number of explants and varied from 1.54 to 6.08 % among the three cultivars and three constructs studied. Several lines of the three sweet orange cultivars analyzed using PCR and Southern blot analysis were genetically transformed with the three constructs evaluated. The histological GUS activity in the leaves indicates that the uidA gene was preferentially expressed in the phloem, which suggests that the use of the three promoters might be adequate for producing HLB-resistant transgenic sweet oranges. The results reported here conclusively demonstrate the preferential expression of GUS in the phloem driven by two heterologous and one homologous gene promoters. Key message The results reported here conclusively demonstrate the preferential expression of GUS in the phloem driven by two heterologous and one homologous

Epidemiology of Usher syndrome in Valencia and Spain.

PubMed

Espinós, C; Millán, J M; Beneyto, M; Nájera, C

1998-01-01

To obtain epidemiological data on the prevalence of the different types of Usher syndrome (US) in Spain, since these data were missing; to estimate the proportion of sporadic cases among simplex families, and calculate the prevalence of the Usher syndrome in a homogeneous population from Eastern Spain (3,875,234 inhabitants) that is representative of the Spanish population. Otological, ophthalmological and genetic studies were performed in 89 US patients from 46 families and subjected to statistical and segregation analysis. 41.6% of them suffered US type I, 46.1% type II, and in 12.3% the classification remains unclear. The estimated prevalence for the Province of Valencia was 4.2/100,000. There was a notable excess of male-only affected multiplex sibships in our sample that could be attributable to an X-linked inheritance. The number of families with USI type was similar to that of families with USII type. The estimated prevalence for the Province of Valencia is in agreement with other reports in which the estimate for the prevalence of US ranges from 1.8 to 6.2/100,000.

Inheritance of flower color in periwinkle: orange-red corolla and white eye.

PubMed

Sreevalli, Y; Kulkarni, R N; Baskaran, K

2002-01-01

The commonly found flower colors in periwinkle (Catharanthus roseus)--pink, white, red-eyed, and pale pink center--are reported to be governed by the epistatic interaction between four genes--A, R, W, and I. The mode of inheritance of an uncommon flower color, orange-red corolla and white eye, was studied by crossing an accession possessing this corolla color with a white flowered variety (Nirmal). The phenotype of the F(1) plants and segregation data of F(2) and backcross generations suggested the involvement of two more interacting and independently inherited genes, one (proposed symbol E) determining the presence or absence of red eye and another (proposed symbol O) determining orange-red corolla.

Consideration for alternative outlet for new citrus hybrids

USDA-ARS?s Scientific Manuscript database

Citrus sinensis (sweet orange, ex. Hamlin, Midsweet, Valencia) is the source of “orange juice” and juice must contain no less than 90% C. sinensis to be marketed as such. Juice produced from Citrus reticulata (mandarins) and C. reticulata hybrids (Orlando, Murcott, Fallglo, Sunburst, Minneola) can b...

Effect of blue LED light intensity on carotenoid accumulation in citrus juice sacs.

PubMed

Zhang, Lancui; Ma, Gang; Yamawaki, Kazuki; Ikoma, Yoshinori; Matsumoto, Hikaru; Yoshioka, Terutaka; Ohta, Satoshi; Kato, Masaya

2015-09-01

In the present study, the effects of blue LED light intensity on carotenoid accumulation and expression of genes related to carotenoid biosynthesis were investigated in the juice sacs of Satsuma mandarin (Citrus unshiu Marc.) and Valencia orange (Citrus sinensis Osbeck) in vitro. The results showed that 100 μmol m(-2)s(-1) blue LED light (100B) was effective for increasing carotenoid content, especially β-cryptoxanthin, in Satsuma mandarin after cultured in vitro for four weeks. In Valencia orange, in contrast, 50 μmol m(-2)s(-1) blue LED light (50B) treatment was effective for inducing carotenoid accumulation through increasing the contents of two major carotenoids, all-trans-violaxanthin and 9-cis-violaxanthin. In addition, gene expression results showed that the simultaneous increases in the expression of genes (CitPSY, CitPDS, CitZDS, CitLCYb2, and CitHYb) involved in producing β,β-xanthophylls were well consistent with the accumulation of β-cryptoxanthin in Satsuma mandarin under 100B, and violaxanthin in Valencia orange under 50B. The results presented herein contribute to further elucidating the regulatory mechanism of carotenoid accumulation by blue LED light. Copyright © 2015 Elsevier GmbH. All rights reserved.

Bioremediation of cooking oil waste using lipases from wastes

PubMed Central

do Prado, Débora Zanoni; Facanali, Roselaine; Marques, Márcia Mayo Ortiz; Nascimento, Augusto Santana; Fernandes, Célio Junior da Costa; Zambuzzi, William Fernando

2017-01-01

Cooking oil waste leads to well-known environmental impacts and its bioremediation by lipase-based enzymatic activity can minimize the high cytotoxic potential. In addition, they are among the biocatalysts most commercialized worldwide due to the versatility of reactions and substrates. However, although lipases are able to process cooking oil wastes, the products generated from this process do not necessarily become less toxic. Thus, the aim of the current study is to analyze the bioremediation of lipase-catalyzed cooking oil wastes, as well as their effect on the cytotoxicity of both the oil and its waste before and after enzymatic treatment. Thus, assessed the post-frying modification in soybean oil and in its waste, which was caused by hydrolysis reaction catalyzed by commercial and home-made lipases. The presence of lipases in the extracts obtained from orange wastes was identified by zymography. The profile of the fatty acid esters formed after these reactions was detected and quantified through gas chromatography and fatty acids profile compared through multivariate statistical analyses. Finally, the soybean oil and its waste, with and without enzymatic treatment, were assessed for toxicity in cytotoxicity assays conducted in vitro using fibroblast cell culture. The soybean oil wastes treated with core and frit lipases through transesterification reaction were less toxic than the untreated oils, thus confirming that cooking oil wastes can be bioremediated using orange lipases. PMID:29073166

Bioremediation of cooking oil waste using lipases from wastes.

PubMed

Okino-Delgado, Clarissa Hamaio; Prado, Débora Zanoni do; Facanali, Roselaine; Marques, Márcia Mayo Ortiz; Nascimento, Augusto Santana; Fernandes, Célio Junior da Costa; Zambuzzi, William Fernando; Fleuri, Luciana Francisco

2017-01-01

Cooking oil waste leads to well-known environmental impacts and its bioremediation by lipase-based enzymatic activity can minimize the high cytotoxic potential. In addition, they are among the biocatalysts most commercialized worldwide due to the versatility of reactions and substrates. However, although lipases are able to process cooking oil wastes, the products generated from this process do not necessarily become less toxic. Thus, the aim of the current study is to analyze the bioremediation of lipase-catalyzed cooking oil wastes, as well as their effect on the cytotoxicity of both the oil and its waste before and after enzymatic treatment. Thus, assessed the post-frying modification in soybean oil and in its waste, which was caused by hydrolysis reaction catalyzed by commercial and home-made lipases. The presence of lipases in the extracts obtained from orange wastes was identified by zymography. The profile of the fatty acid esters formed after these reactions was detected and quantified through gas chromatography and fatty acids profile compared through multivariate statistical analyses. Finally, the soybean oil and its waste, with and without enzymatic treatment, were assessed for toxicity in cytotoxicity assays conducted in vitro using fibroblast cell culture. The soybean oil wastes treated with core and frit lipases through transesterification reaction were less toxic than the untreated oils, thus confirming that cooking oil wastes can be bioremediated using orange lipases.

Influence of different organic fertilizers on quality parameters and the delta(15)N, delta(13)C, delta(2)H, delta(34)S, and delta(18)O values of orange fruit (Citrus sinensis L. Osbeck).

PubMed

Rapisarda, Paolo; Camin, Federica; Fabroni, Simona; Perini, Matteo; Torrisi, Biagio; Intrigliolo, Francesco

2010-03-24

To investigate the influence of different types of fertilizers on quality parameters, N-containing compounds, and the delta(15)N, delta(13)C, delta(2)H, delta (34)S, and delta(18)O values of citrus fruit, a study was performed on the orange fruit cv. 'Valencia late' (Citrus sinensis L. Osbeck), which was harvested in four plots (three organic and one conventional) located on the same farm. The results demonstrated that different types of organic fertilizers containing the same amount of nitrogen did not effect important changes in orange fruit quality parameters. The levels of total N and N-containing compounds such as synephrine in fruit juice were not statistically different among the different treatments. The delta(15)N values of orange fruit grown under fertilizer derived from animal origin as well as from vegetable compost were statistically higher than those grown with mineral fertilizer. Therefore, delta(15)N values can be used as an indicator of citrus fertilization management (organic or conventional), because even when applied organic fertilizers are of different origins, the natural abundance of (15)N in organic citrus fruit remains higher than in conventional ones. These treatments also did not effect differences in the delta(13)C, delta(2)H, delta(34)S, and delta(18)O values of fruit.

Cotyledon density measurements on valencia peanuts grown in the Southwest United States as a tool for developing food products

USDA-ARS?s Scientific Manuscript database

Valencia peanuts (Arachis hypogaea L. ssp. fastigiata) are able to complete seed development in an environment where extreme temperature variation and water deficit are common and growing season is short. Valencia seed can command a premium in food products as consumers like special properties like...

Growing Oranges. People on the Farm.

ERIC Educational Resources Information Center

Department of Agriculture, Washington, DC. Office of Governmental and Public Affairs.

This booklet, one in a series about life on modern farms, describes the daily lives of three orange growers in Florida and one in California. Two of the Florida orange growers also have other jobs, one as manager of a citrus cooperative and the other as a citrus insurance salesman. The operations of orange groves, the care and picking of oranges,…

Effect of edible coatings with essential oils on the quality of red raspberries over shelf-life.

PubMed

Gomes, Marcos de Souza; Cardoso, Maria das Graças; Guimarães, Ana Clara Garcia; Guerreiro, Adriana Cavaco; Gago, Custódia Maria Luís; Vilas Boas, Eduardo Valério de Barros; Dias, Cristina Maria Barrocas; Manhita, Ana Cristina Cabaça; Faleiro, Maria Leonor; Miguel, Maria Graça Costa; Antunes, Maria Dulce Carlos

2017-02-01

The objective of the present work was to develop strategies for increasing the shelf-life of red raspberries (Rubus idaeus L.), by preventing microorganism growth. Fruits coated with alginate plus lemon essential oil (0.2%) or orange essential oil (0.1%) after 15 days of storage had less red skin than the remaining samples. The less red color verified in these samples was also coincident with the lower concentration of anthocyanins at the end of the experiment as well as the lower capacity for scavenging ABTS free radicals or quenching singlet oxygen. Cyanidin and pelargonidin glucosides were found in raspberries fruits. The edible coatings supplemented with the essential oil of orange either at 0.1% or 0.2% were very efficient for controlling yeast and mold growth after 15 days of storage. To control the development of aerobic mesophilic bacteria the use of essential oil of lemon 0.2% and essential oil of orange 0.1% were the most efficient. The application of the film improved post-harvest quality of raspberry, since the addition of essential oils of citrus films promoted to the inhibitory effect of fungi and bacteria growth after 15 days of storage, without changing quality parameters. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

Antimicrobial and antiplasmid activities of essential oils.

PubMed

Schelz, Zsuzsanna; Molnar, Joseph; Hohmann, Judit

2006-06-01

The antimicrobial and antiplasmid activities of essential oils (orange oil, eucalyptus oil, fennel oil, geranium oil, juniper oil, peppermint oil, rosemary oil, purified turpentine oil, thyme oil, Australian tea tree oil) and of menthol, the main component of peppermint oil, were investigated. The antimicrobial activities were determined on the Gram (+) Staphylococcus epidermidis and the Gram (-) Escherichia coli F'lac K12 LE140, and on two yeast Saccharomyces cerevisiae 0425 delta/1 and 0425 52C strains. The antiplasmid activities were investigated on E. coli F'lac bacterial strain. Each of the oils exhibited antimicrobial activity and three of them antiplasmid action. The interaction of peppermint oil and menthol with the antibiotics was studied on the same bacterial strain with the checkerboard method. Peppermint oil and menthol displayed additive synergy with oxytetracycline. A new mechanism of plasmid curing was established for one of the oil components.

Compositional Shift in Fatty Acid Profiles of Lipids Obtained from Oleaginous Yeasts upon the Addition of Essential Oil from Citrus sinensis L.

PubMed

Uprety, Bijaya K; Rakshit, Sudip K

2017-12-01

Tailoring lipids from oleaginous yeasts to contain specific types of fatty acid is of considerable interest to food, fuel, and pharmaceutical industries. In this study, the essential oil obtained from Citrus sinesus L. has been used to alter the fatty acid composition of two common oleaginous yeasts, Rhodosporidium toruloides and Cryptococcus curvatus. With increasing levels of essential oil in the medium, the metabolic flux of the fatty acid biosynthesis pathway shifted towards saturated fatty acid production. Essential oil reduced the activities of elongase and ∆9 desaturase. This made the lipid obtained from both these yeasts rich in saturated fatty acids. At certain specific concentrations of the essential oil in the medium, the lipid obtained from R. toruloides and C. curvatus cultures was similar to mahuwa butter and palm oil, respectively. Limonene is the major constituents of orange essential oil. Its effect on one of the oleaginous yeasts, R. toruloides, was also studied separately. Effects similar to orange essential oil were obtained with limonene. Thus, we can conclude that limonene in orange essential oil brings about compositional change of microbial lipid produced in this organism.

Technical Note: Dosimetry of Leipzig and Valencia applicators without the plastic cap

DOE Office of Scientific and Technical Information (OSTI.GOV)

Granero, D., E-mail: dgranero@eresa.com; Candela-Juan, C.; Vijande, J.

2016-05-15

Purpose: High dose rate (HDR) brachytherapy for treatment of small skin lesions using the Leipzig and Valencia applicators is a widely used technique. These applicators are equipped with an attachable plastic cap to be placed during fraction delivery to ensure electronic equilibrium and to prevent secondary electrons from reaching the skin surface. The purpose of this study is to report on the dosimetric impact of the cap being absent during HDR fraction delivery, which has not been explored previously in the literature. Methods: GEANT4 Monte Carlo simulations (version 10.0) have been performed for the Leipzig and Valencia applicators with andmore » without the plastic cap. In order to validate the Monte Carlo simulations, experimental measurements using radiochromic films have been done. Results: Dose absorbed within 1 mm of the skin surface increases by a factor of 1500% for the Leipzig applicators and of 180% for the Valencia applicators. Deeper than 1 mm, the overdosage flattens up to a 10% increase. Conclusions: Differences of treating with or without the plastic cap are significant. Users must check always that the plastic cap is in place before any treatment in order to avoid overdosage of the skin. Prior to skin HDR fraction delivery, the timeout checklist should include verification of the cap placement.« less

Operational forecasting of daily temperatures in the Valencia Region. Part II: minimum temperatures in winter.

NASA Astrophysics Data System (ADS)

Gómez, I.; Estrela, M.

2009-09-01

Extreme temperature events have a great impact on human society. Knowledge of minimum temperatures during winter is very useful for both the general public and organisations whose workers have to operate in the open, e.g. railways, roadways, tourism, etc. Moreover, winter minimum temperatures are considered a parameter of interest and concern since persistent cold-waves can affect areas as diverse as public health, energy consumption, etc. Thus, an accurate forecasting of these temperatures could help to predict cold-wave conditions and permit the implementation of strategies aimed at minimizing the negative effects that low temperatures have on human health. The aim of this work is to evaluate the skill of the RAMS model in determining daily minimum temperatures during winter over the Valencia Region. For this, we have used the real-time configuration of this model currently running at the CEAM Foundation. To carry out the model verification process, we have analysed not only the global behaviour of the model for the whole Valencia Region, but also its behaviour for the individual stations distributed within this area. The study has been performed for the winter forecast period from 1 December 2007 - 31 March 2008. The results obtained are encouraging and indicate a good agreement between the observed and simulated minimum temperatures. Moreover, the model captures quite well the temperatures in the extreme cold episodes. Acknowledgement. This work was supported by "GRACCIE" (CSD2007-00067, Programa Consolider-Ingenio 2010), by the Spanish Ministerio de Educación y Ciencia, contract number CGL2005-03386/CLI, and by the Regional Government of Valencia Conselleria de Sanitat, contract "Simulación de las olas de calor e invasiones de frío y su regionalización en la Comunidad Valenciana" ("Heat wave and cold invasion simulation and their regionalization at Valencia Region"). The CEAM Foundation is supported by the Generalitat Valenciana and BANCAIXA (Valencia

Operational forecasting of daily temperatures in the Valencia Region. Part I: maximum temperatures in summer.

NASA Astrophysics Data System (ADS)

Gómez, I.; Estrela, M.

2009-09-01

Extreme temperature events have a great impact on human society. Knowledge of summer maximum temperatures is very useful for both the general public and organisations whose workers have to operate in the open, e.g. railways, roadways, tourism, etc. Moreover, summer maximum daily temperatures are considered a parameter of interest and concern since persistent heat-waves can affect areas as diverse as public health, energy consumption, etc. Thus, an accurate forecasting of these temperatures could help to predict heat-wave conditions and permit the implementation of strategies aimed at minimizing the negative effects that high temperatures have on human health. The aim of this work is to evaluate the skill of the RAMS model in determining daily maximum temperatures during summer over the Valencia Region. For this, we have used the real-time configuration of this model currently running at the CEAM Foundation. To carry out the model verification process, we have analysed not only the global behaviour of the model for the whole Valencia Region, but also its behaviour for the individual stations distributed within this area. The study has been performed for the summer forecast period of 1 June - 30 September, 2007. The results obtained are encouraging and indicate a good agreement between the observed and simulated maximum temperatures. Moreover, the model captures quite well the temperatures in the extreme heat episodes. Acknowledgement. This work was supported by "GRACCIE" (CSD2007-00067, Programa Consolider-Ingenio 2010), by the Spanish Ministerio de Educación y Ciencia, contract number CGL2005-03386/CLI, and by the Regional Government of Valencia Conselleria de Sanitat, contract "Simulación de las olas de calor e invasiones de frío y su regionalización en la Comunidad Valenciana" ("Heat wave and cold invasion simulation and their regionalization at Valencia Region"). The CEAM Foundation is supported by the Generalitat Valenciana and BANCAIXA (Valencia, Spain).

Response of citrus and other selected plant species to simulated HCL - acid rain

NASA Technical Reports Server (NTRS)

Knott, W. M.; Heagle, A. S.

1980-01-01

Mature valencia orange trees were sprayed with hydrochloric acid solutions (pH 7.8, 2.0, 1.0, and 0.5) in the field at the full bloom stage and at one month after fruit set. Potted valencia orange and dwarf citrus trees, four species of plants native to Merritt Island, and four agronomic species were exposed to various pH levels of simulated acid rain under controlled conditions. The acid rain was generated from dilutions of hydrochloric acid solutions or by passing water through an exhaust generated by burning solid rocket fuel. The plants were injured severely at pH levels below 1.0, but showed only slight injury at pH levels of 2.0 and above. Threshold injury levels were between 2.0 and 3.0 pH. The sensitivity of the different plant species to acid solutions was similar. Foliar injury symptoms were representative of acid rain including necrosis of young tissue, isolated necrotic spots or patches, and leaf abscission. Mature valencia orange trees sprayed with concentrations of 1.0 pH and 0.5 pH in the field had reduced fruit yields for two harvests after the treatment. All experimental trees were back to full productivity by the third harvest after treatment.

The Valencia trough and the origin of the western Mediterranean basins

NASA Astrophysics Data System (ADS)

Vegas, R.

1992-03-01

Evolutionary models for the Valencia trough must be necessarily related to the Neogene-Present geodynamics of the western Mediterranean basins. All these basins occupy new space created in the wake of the westward translation of the Alboran block and the counter-clockwise rotation of the Corso-Sardinian block. This escape-tectonics, microplate dispersal, model can account for the co-existence and progressive migration of compressional and extensional strain fields within the Africa-Europe broad zone of convergence. In this escape-tectonics model, the Valencia trough has resulted in a complex evolution which includes: (1) latest Oligocene-Early Miocene rifting along the Catalan-Valencian margin due to the opening of the Gulf of Lions; (2) almost simultaneous, Early Miocene, transpressive thrusting in the Balearic margin related to the initiation of displacement of the Alboran block; and (3) Late Miocene generalized extension as a consequence of the opening of the South Balearic basin.

Terpene Down-Regulation Triggers Defense Responses in Transgenic Orange Leading to Resistance against Fungal Pathogens1[W

PubMed Central

Rodríguez, Ana; Shimada, Takehiko; Cervera, Magdalena; Alquézar, Berta; Gadea, José; Gómez-Cadenas, Aurelio; De Ollas, Carlos José; Rodrigo, María Jesús; Zacarías, Lorenzo; Peña, Leandro

2014-01-01

Terpenoid volatiles are isoprene compounds that are emitted by plants to communicate with the environment. In addition to their function in repelling herbivores and attracting carnivorous predators in green tissues, the presumed primary function of terpenoid volatiles released from mature fruits is the attraction of seed-dispersing animals. Mature oranges (Citrus sinensis) primarily accumulate terpenes in peel oil glands, with d-limonene accounting for approximately 97% of the total volatile terpenes. In a previous report, we showed that down-regulation of a d-limonene synthase gene alters monoterpene levels in orange antisense (AS) fruits, leading to resistance against Penicillium digitatum infection. A global gene expression analysis of AS versus empty vector (EV) transgenic fruits revealed that the down-regulation of d-limonene up-regulated genes involved in the innate immune response. Basal levels of jasmonic acid were substantially higher in the EV compared with AS oranges. Upon fungal challenge, salicylic acid levels were triggered in EV samples, while jasmonic acid metabolism and signaling were drastically increased in AS orange peels. In nature, d-limonene levels increase in orange fruit once the seeds are fully viable. The inverse correlation between the increase in d-limonene content and the decrease in the defense response suggests that d-limonene promotes infection by microorganisms that are likely involved in facilitating access to the pulp for seed-dispersing frugivores. PMID:24192451

21 CFR 146.137 - Frozen orange juice.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen orange juice. 146.137 Section 146.137 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR... Beverages § 146.137 Frozen orange juice. (a) Frozen orange juice is orange juice as defined in § 146.135...

21 CFR 74.250 - Orange B.

Code of Federal Regulations, 2013 CFR

2013-04-01

... FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR ADDITIVES SUBJECT TO CERTIFICATION Foods § 74.250 Orange B. (a) Identity. (1) The color additive Orange B is.... (2) The diluents in color additive mixtures for food use containing Orange B are limited to those...

21 CFR 74.250 - Orange B.

Code of Federal Regulations, 2012 CFR

2012-04-01

... FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR ADDITIVES SUBJECT TO CERTIFICATION Foods § 74.250 Orange B. (a) Identity. (1) The color additive Orange B is.... (2) The diluents in color additive mixtures for food use containing Orange B are limited to those...

21 CFR 74.250 - Orange B.

Code of Federal Regulations, 2011 CFR

2011-04-01

... FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR ADDITIVES SUBJECT TO CERTIFICATION Foods § 74.250 Orange B. (a) Identity. (1) The color additive Orange B is.... (2) The diluents in color additive mixtures for food use containing Orange B are limited to those...

21 CFR 74.250 - Orange B.

Code of Federal Regulations, 2014 CFR

2014-04-01

... FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR ADDITIVES SUBJECT TO CERTIFICATION Foods § 74.250 Orange B. (a) Identity. (1) The color additive Orange B is.... (2) The diluents in color additive mixtures for food use containing Orange B are limited to those...

Philadelphus L.: mock orange

Treesearch

Nancy L. Shaw; Emerenciana G. Hurd; Peter F. Stickney

2008-01-01

The mock oranges - Philadelphus spp. - have been placed in several families: Saxifragaceae (Harrington 1954), Hydrangeaceae (Hitchcock and others 1961), and more recently, the Philadelphaceae (Hickman 1993). Hydrangeaceae, however, is the most widely accepted placement (Cronquist and others 1997; USDA NRCS 2001). There are about 50 to 65 species of mock orange,...

Wrong postural hygiene and ergonomics in dental students of the University of Valencia (Spain) (part I).

PubMed

Cervera-Espert, J; Pascual-Moscardó, A; Camps-Alemany, I

2018-02-01

Failure to adopt a correct working posture can lead to occupational diseases. Evaluate knowledge in relation to ergonomics about BHOP concept and its application to routine clinical practice amongst undergraduate and postgraduate dental students in the University of Valencia (Valencia, Spain). A study based on interviews of undergraduate and postgraduate dental students in the University of Valencia (Valencia, Spain) was carried out. The information from a total of 336 interviews was used for the statistical analysis, differentiating according to gender and academic year: knowledge of ergonomics, pain prevalence and antecedents, assessment of the possible necessity for improved training in ergonomics, and evaluation of postural hygiene. Only 28.6% of the students were found to sit correctly in the dentist chair. Furthermore, in the opinion of the students, very few subjects during the career afforded adequate teaching in relation to ergonomics and working posture. The analysis of postural hygiene showed great variability. There were no significant differences in posture between males and females, although some incorrect postures appeared to be associated with the academic year (P<.05). However, no significant improvements in postural hygiene were noted on progressing from one academic year to the next. The students in our study were not familiar with the principles of ergonomics and did not sit correctly in the dentist chair. Improved training in this field is required in dental school. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Variability of arginine content and yield components in Valencia peanut germplasm.

PubMed

Aninbon, Chorkaew; Jogloy, Sanun; Vorasoot, Nimitr; Nuchadomrong, Suporn; Holbrook, C Corley; Kvien, Craig; Puppala, Naveen; Patanothai, Aran

2017-06-01

Peanut seeds are rich in arginine, an amino acid that has several positive effects on human health. Establishing the genetic variability of arginine content in peanut will be useful for breeding programs that have high arginine as one of their goals. The objective of this study was to evaluate the variation of arginine content, pods/plant, seeds/pod, seed weight, and yield in Valencia peanut germplasm. One hundred and thirty peanut genotypes were grown under field condition for two years. A randomized complete block design with three replications was used for this study. Arginine content was analyzed in peanut seeds at harvest using spectrophotometry. Yield and yield components were recorded for each genotype. Significant differences in arginine content and yield components were found in the tested Valencia peanut germplasm. Arginine content ranged from 8.68-23.35 μg/g seed. Kremena was the best overall genotype of high arginine content, number of pods/plant, 100 seed weight and pod yield.

Nutraceutical delivery systems: resveratrol encapsulation in grape seed oil nanoemulsions formed by spontaneous emulsification.

PubMed

Davidov-Pardo, Gabriel; McClements, David Julian

2015-01-15

The aim of this work was to fabricate nanoemulsions-based delivery systems to encapsulate resveratrol. Nanoemulsions were formed using spontaneous emulsification method: 10% oil phase (grape seed oil plus orange oil) and 10% surfactant (Tween 80) were titrated into 80% aqueous phase. An optimum orange oil-to-grape seed oil ratio of 1:1(w/w) formed small droplets (d ≈ 100 nm) with good stability to droplet growth. The maximum amount of resveratrol that could be dissolved in the oil phase was 120 ± 10 μg/ml. The effect of droplet size on the chemical stability of encapsulated resveratrol was examined by preparing systems with different mean droplet diameters of 220 ± 2; 99 ± 3; and 45 ± 0.4 nm. Encapsulation of resveratrol improved its chemical stability after exposure to UV-light: 88% retention in nanoemulsions compared to 50% in dimethylsulphoxide (DMSO). This study showed that resveratrol could be encapsulated within low-energy nanoemulsion-based delivery systems and protected against degradation. Copyright © 2014 Elsevier Ltd. All rights reserved.

Valencia College, No. 1 Nationwide: It's All about "Student Experience"

ERIC Educational Resources Information Center

Adam, Michelle

2012-01-01

Twelve years ago, Sanford Shugart took the helm at Valencia College. This school of 70,000-plus students in Orlando, FL, seemed like any other community college, but Shugart's reason for being there, and the change this college was about to undergo, was anything but ordinary. Before his arrival, the school had begun a process called Campus…

Happy orang-utans live longer lives.

PubMed

Weiss, Alexander; Adams, Mark J; King, James E

2011-12-23

Nonhuman primate ageing resembles its human counterpart. Moreover, ratings of subjective well-being traits in chimpanzees, orang-utans and rhesus macaques are similar to those of humans: they are intercorrelated, heritable, and phenotypically and genetically related to personality. We examined whether, as in humans, orang-utan subjective well-being was related to longer life. The sample included 184 zoo-housed orang-utans followed up for approximately 7 years. Age, sex, species and number of transfers were available for all subjects and 172 subjects were rated on at least one item of a subjective well-being scale. Of the 31 orang-utans that died, 25 died a mean of 3.4 years after being rated. Even in a model that included, and therefore, statistically adjusted for, sex, age, species and transfers, orang-utans rated as being "happier" lived longer. The risk differential between orang-utans that were one standard deviation above and one standard deviation below baseline in subjective well-being was comparable with approximately 11 years in age. This finding suggests that impressions of the subjective well-being of captive great apes are valid indicators of their welfare and longevity.

Toxicities of Selected Essential Oils, Silicone Oils, and Paraffin Oil against the Common Bed Bug (Hemiptera: Cimicidae).

PubMed

Zha, Chen; Wang, Changlu; Li, Andrew

2018-02-09

The common bed bug [Cimex lectularius L. (Hemiptera: Cimicidae)] and tropical bed bug [Cimex hemipterus F. (Hemiptera: Cimicidae)] resurged in the United States and many other countries over the past decades. The need for safe and effective bed bug control products propelled the development of numerous 'green insecticides', mostly with essential oils listed as active ingredients. Various inorganic and organic oils also were used for bed bug management. However, there are no published studies on their toxicities against bed bugs. In this study, we screened 18 essential oils, three silicone oils, and paraffin oil (C5-20 paraffins) for their toxicities against bed bugs. All the oils exhibited insecticidal activity in topical assays. Their toxicities varied significantly; all of the evaluated essential oils were less effective than silicone oils and paraffin oil. The LD50 values of the most effective essential oil (blood orange), paraffin oil, and the most effective silicone oil (dodecamethylpentasiloxane) are 0.184 ± 0.018, 0.069 ± 0.012, and 0.036 ± 0.005 mg per bug, respectively. Direct spray of 1% water solution of 3-[hydroxy (polyethyleneoxy) propyl] heptamethyltrisiloxane, the only silicone oil that mixes well with water, resulted in 92% bed bug mortality after 1 d. Results of this study indicate silicone oils and paraffin oil have the potential to be used as safer alternative bed bug control materials. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

The use of thyme and orange essential oils blend to improve quality traits of marinated chicken meat.

PubMed

Rimini, Simone; Petracci, Massimiliano; Smith, Douglas P

2014-08-01

Poultry meat contains large quantities of polyunsaturated fatty acids, which lead to oxidative deterioration. Plant essential oils (EO) and natural compounds, with antioxidant properties, may be used to alleviate this problem. Two replications were conducted to evaluate the effects of a mixture (1:1) of thyme and orange oils (EO) on the quality characteristics and the oxidative stability of chicken meat (breast and wing). For each replication, 24 fresh breast fillets and 24 wings were procured from a local grocery store. The EO were added to marinade solution to achieve a final concentration of 0.55% sodium chloride, 0.28% polyphosphate, and 0.05% wt/vol of EO blend. Breasts and wings were split in 2 different groups with homogenous pH and lightness and vacuum tumbled in 2 treatments, a 0.5% EO and a control (CON, no EO). Each group was tested for pH, Commission Internationale d'Eclairage color (lightness, L*; redness, a*; yellowness, b*), moisture content, marinade uptake, purge loss, cook yield, and shear force. Susceptibility to lipid oxidation was determined on fresh and frozen meat by TBA reactive substance analysis (induced oxidation from 0 to 150 min at 37°C). The EO breasts had lower purge loss compared with CON meat. Breast did not show any color, pH, marinade uptake, cooking yield, shear force, or moisture differences due to treatment, although cooked EO breast was slightly less red than CON. The EO wings presented higher a* and b* values after marination and lower purge loss and shear force than CON. No differences were detected on wings for color, pH, marinade uptake, cooking yield, or moisture between EO and CON wings. Both fresh and frozen EO breasts and EO wings were less susceptible to the lipid oxidation during all induced oxidation times compared with CON breasts and wings. In conclusion, EO had a positive effect on broiler breast and wing lipid oxidation without negatively affecting meat quality traits. © Poultry Science Association Inc.

Heavy metals in navel orange orchards of Xinfeng County and their transfer from soils to navel oranges.

PubMed

Cheng, Jinjin; Ding, Changfeng; Li, Xiaogang; Zhang, Taolin; Wang, Xingxiang

2015-12-01

This study investigated heavy metal concentrations in soils and navel oranges of Xinfeng County, a well-known navel orange producing area of China. The results showed that the average concentrations of lead (Pb), cadmium (Cd), chromium (Cr), arsenic (As) and mercury (Hg) in orchard soils all increased compared to the regional background values, especially for Cd, which increased by 422%. When compared to the Chinese Environmental Quality Standard for soil (GB15618-1995), Pb, Cr and Hg concentrations in all orchard soil samples were below the limit standards, but Cd concentrations in 24 soil samples (21%) and As concentrations in 8 soil samples (7%) exceeded the limit standards. However, concentrations of all heavy metals in navel orange pulps were within the National Food Safety Standard of China (GB 2762-2012). Dietary risk assessment also showed that the exposure to these five heavy metals by consumption of navel oranges could hardly pose adverse health effects on adults and children. Since the range and degree of soil Cd pollution was widest and the most severe of all, Cd was taken as an example to reveal the transfer characteristics of heavy metals in soil-navel orange system. Cd concentrations in different organs of navel orange trees decreased in the following order: root>leaf>peel>pulp. That navel oranges planted in the Cd contaminated soils were within the national food safety standard was mainly due to the low transfer factor for Cd from soil to pulp (TFpulp). Further studies showed that TFpulp was significantly negatively correlated with soil pH, organic carbon (OC) and cation exchange capacity (CEC). Based on these soil properties, a prediction equation for TFpulp was established, which indicated that the risk for Cd concentration of navel orange pulp exceeding the national food limit is generally low, when soil Cd concentration is below 7.30 mg/kg. If appropriate actions are taken to increase soil pH, OC and CEC, Cd concentrations in navel orange pulps

Evaluating the conservation impact of an innovative zoo-based educational campaign: 'Don't Palm Us Off' for orang-utan conservation.

PubMed

Pearson, Elissa L; Lowry, Rachel; Dorrian, Jillian; Litchfield, Carla A

2014-01-01

With significant biodiversity loss occurring presently, increased emphasis is being placed upon the capacity of zoos to contribute to species conservation. This paper evaluates an innovative conservation education campaign 'Don't Palm Us Off' implemented at Melbourne Zoo, Australia. This sought to address a lack of public awareness regarding palm oil (the product most threatening the survival of the orang-utan) and to create public support for mandatory labeling of palm oil on food products, allowing for informed consumer purchasing. Communication tools utilized included an educational video presentation played on-site, as well as You Tube video, celebrity ambassadors, and social media. Evaluation took place across four time-points: baseline, mid-point, conclusion (12 months), and follow-up. Zoo visitors (N = 403) were randomly selected whilst visiting the orang-utan exhibit, completing a questionnaire regarding knowledge about orangutans, attitudes toward orangutans, support for palm oil labeling, previous conservation behavior, and intentions for future behavior. Results revealed significant increases in palm oil awareness; attitudes toward orangutans; support for palm oil labeling; and indicating labeling would influence purchasing behavior, at all times relative to baseline (P < 0.01). There were also significant increases in self-reported conservation behavior at the end of the campaign and follow-up (P < 0.05). In excess of 160,000 people additionally signed an associated petition for mandatory palm oil labeling. Overall the findings support the efficacy of this multi-faceted initiative; highlighting the importance of continued innovation in zoo-based conservation education and practice (including the integration of emerging technologies with traditional on-site education) to maximize contributions to species conservation. © 2014 The Authors. Zoo Biology Published by Wiley Periodicals, Inc.

Orange Button Solar Data Exchange | Energy Analysis | NREL

Science.gov Websites

Orange Button Solar Data Exchange Orange Button Solar Data Exchange The new Orange Button Solar Data Exchange tool serves as an online resource for the solar industry to share, sell, or retrieve solar data and connect with colleagues. Screenshot image of Orange Button data website home page. The

21 CFR 146.140 - Pasteurized orange juice.

Code of Federal Regulations, 2010 CFR

2010-04-01

... and Beverages § 146.140 Pasteurized orange juice. (a) Pasteurized orange juice is the food prepared... section. One or more of the optional sweetening ingredients listed in paragraph (c) of this section may be... orange juice soluble solids, exclusive of the solids of any added optional sweetening ingredients, and...

21 CFR 146.140 - Pasteurized orange juice.

Code of Federal Regulations, 2012 CFR

2012-04-01

... and Beverages § 146.140 Pasteurized orange juice. (a) Pasteurized orange juice is the food prepared... section. One or more of the optional sweetening ingredients listed in paragraph (c) of this section may be... orange juice soluble solids, exclusive of the solids of any added optional sweetening ingredients, and...

21 CFR 146.140 - Pasteurized orange juice.

Code of Federal Regulations, 2014 CFR

2014-04-01

... and Beverages § 146.140 Pasteurized orange juice. (a) Pasteurized orange juice is the food prepared... section. One or more of the optional sweetening ingredients listed in paragraph (c) of this section may be... orange juice soluble solids, exclusive of the solids of any added optional sweetening ingredients, and...

21 CFR 146.140 - Pasteurized orange juice.

Code of Federal Regulations, 2011 CFR

2011-04-01

... and Beverages § 146.140 Pasteurized orange juice. (a) Pasteurized orange juice is the food prepared... section. One or more of the optional sweetening ingredients listed in paragraph (c) of this section may be... orange juice soluble solids, exclusive of the solids of any added optional sweetening ingredients, and...

21 CFR 146.140 - Pasteurized orange juice.

Code of Federal Regulations, 2013 CFR

2013-04-01

... and Beverages § 146.140 Pasteurized orange juice. (a) Pasteurized orange juice is the food prepared... section. One or more of the optional sweetening ingredients listed in paragraph (c) of this section may be... orange juice soluble solids, exclusive of the solids of any added optional sweetening ingredients, and...

Essential Oils, Part IV: Contact Allergy.

PubMed

de Groot, Anton C; Schmidt, Erich

2016-01-01

Nearly 80 essential oils (including 2 jasmine absolutes) have caused contact allergy. Fifty-five of these have been tested in consecutive patients suspected of contact dermatitis, and nine (laurel, turpentine, orange, tea tree, citronella, ylang-ylang, sandalwood, clove, and costus root) showed greater than 2% positive patch test reactions. Relevance data are generally missing or inadequate. Most reactions are caused by application of pure oils or high-concentration products. The clinical picture depends on the responsible product. Occupational contact dermatitis may occur in professionals performing massages. The (possible) allergens in essential oils are discussed. Several test allergens are available, but patients should preferably be tested with their own products. Co-reactivity with other essential oils and the fragrance mix is frequent, which may partly be explained by common ingredients. Patch test concentrations for essential oils are suggested.

[Prevalence of indirect sharing of drug-injecting paraphernalia in Galicia, Madrid, Seville and Valencia [Spain

PubMed

Bravo, María J; Royuela, Luis; Barrio, Gregorio; Rodríguez-Arenas, María A; de la Fuente, Luis

2004-01-01

To study drug-injecting practices, particularly indirect sharing of injecting paraphernalia (ISIP), and sexual risk behavior. We performed a cross-sectional study of 1638 users of needle exchange programs (NEPs). Different types of ISIP were studied: taking diluted drugs in a syringe used by others, placing the needle in a recipient with other used needles, and reusing cleaning liquid previously used by others. The prevalence of injecting with syringes already used by others was 16% in Galicia, 4.7% in Madrid, 17.6% in Seville and 13.2% in Valencia (p < 0.001). With geographical variations, other types of ISIP (Galicia: 32.4%; Madrid: 28.5%; Seville: 42.6%; Valencia: 27.4% -p < 0.001-) were more frequent than injecting with syringes already used by others (Galicia: 32.4%; Madrid: 28.5%; Seville: 42.6%; Valencia: 27.4% -p < 0.001-). The percentage not injecting with syringes used by others but performing ISIP was 21.7%, 25.3%, 28.2% and 18.1% (p < 0.01) respectively. In all geographical areas, sexual risk practices were more prevalent with steady sex partners (68.6%, 72.0%, 77.8%, 72.8% [NS]) than with casual partners (36.6%, 40.9%, 37.9%, 23.9% [NS]). Among injectors with a stable partner, 81.3% in Galicia, 75.9% in Madrid, 86.1% in Seville and 79.7% in Valencia reported that his/her serological status was negative for HIV or was unknown (p < 0.001). ISIP is more prevalent than injection with syringes already used by others. For a substantial percentage of injectors, ISIP is the only risk practice. ISIP and the low use of condoms, particularly with steady partners, could be a contributory factor to the spread of HIV, hepatitis C virus, and hepatitis B virus infection.

Risk assessment and monitoring programme of nitrates through vegetables in the Region of Valencia (Spain).

PubMed

Quijano, Leyre; Yusà, Vicent; Font, Guillermina; McAllister, Claudia; Torres, Concepción; Pardo, Olga

2017-02-01

This study was carried out to determine current levels of nitrate in vegetables marketed in the Region of Valencia (Spain) and to estimate the toxicological risk associated with their intake. A total of 533 samples of seven vegetable species were studied. Nitrate levels were derived from the Valencia Region monitoring programme carried out from 2009 to 2013 and food consumption levels were taken from the first Valencia Food Consumption Survey, conducted in 2010. The exposure was estimated using a probabilistic approach and two scenarios were assumed for left-censored data: the lower-bound scenario, in which unquantified results (below the limit of quantification) were set to zero and the upper-bound scenario, in which unquantified results were set to the limit of quantification value. The exposure of the Valencia consumers to nitrate through the consumption of vegetable products appears to be relatively low. In the adult population (16-95 years) the P99.9 was 3.13 mg kg -1 body weight day -1 and 3.15 mg kg -1 body weight day -1 in the lower bound and upper bound scenario, respectively. On the other hand, for young people (6-15 years) the P99.9 of the exposure was 4.20 mg kg -1 body weight day -1 and 4.40 mg kg -1 body weight day -1 in the lower bound and upper bound scenario, respectively. The risk characterisation indicates that, under the upper bound scenario, 0.79% of adults and 1.39% of young people can exceed the Acceptable Daily Intake of nitrate. This percentage could join the vegetable extreme consumers (such as vegetarians) of vegetables. Overall, the estimated exposures to nitrate from vegetables are unlikely to result in appreciable health risks. Copyright © 2016 Elsevier Ltd. All rights reserved.

21 CFR 146.141 - Canned orange juice.

Code of Federal Regulations, 2010 CFR

2010-04-01

... Beverages § 146.141 Canned orange juice. (a) Canned orange juice is the food prepared from orange juice as... the deoiling operation may be added back. One or more of the optional sweetening ingredients named in... milliliters of juice is not less than 9 to 1. (b) The optional sweetening ingredients referred to in paragraph...

21 CFR 146.141 - Canned orange juice.

Code of Federal Regulations, 2014 CFR

2014-04-01

... Beverages § 146.141 Canned orange juice. (a) Canned orange juice is the food prepared from orange juice as... the deoiling operation may be added back. One or more of the optional sweetening ingredients named in... milliliters of juice is not less than 9 to 1. (b) The optional sweetening ingredients referred to in paragraph...

21 CFR 146.141 - Canned orange juice.

Code of Federal Regulations, 2012 CFR

2012-04-01

... Beverages § 146.141 Canned orange juice. (a) Canned orange juice is the food prepared from orange juice as... the deoiling operation may be added back. One or more of the optional sweetening ingredients named in... milliliters of juice is not less than 9 to 1. (b) The optional sweetening ingredients referred to in paragraph...

21 CFR 146.141 - Canned orange juice.

Code of Federal Regulations, 2013 CFR

2013-04-01

... Beverages § 146.141 Canned orange juice. (a) Canned orange juice is the food prepared from orange juice as... the deoiling operation may be added back. One or more of the optional sweetening ingredients named in... milliliters of juice is not less than 9 to 1. (b) The optional sweetening ingredients referred to in paragraph...

21 CFR 146.141 - Canned orange juice.

Code of Federal Regulations, 2011 CFR

2011-04-01

... Beverages § 146.141 Canned orange juice. (a) Canned orange juice is the food prepared from orange juice as... the deoiling operation may be added back. One or more of the optional sweetening ingredients named in... milliliters of juice is not less than 9 to 1. (b) The optional sweetening ingredients referred to in paragraph...

New early instrumental series since the beginning of the 19th century in eastern Iberia (Valencia, Spain)

NASA Astrophysics Data System (ADS)

Sanchez-Lorenzo, Arturo; Barriendos, Mariano; Guinaldo, Elena; Lopez-Bustins, Joan A.

2010-05-01

Early instrumental series are the main source for climate information in the 18th and the first part of the 19th century, which is when systematic meteorological observations started in most national meteorological services. The first continuous series in Spain starts in 1780 in Barcelona due to meteorological observations made by the medical doctor Francisco Salvá Campillo. Moreover, only two other series have been recovered at the present in Spain: Madrid and Cádiz/San Fernando. Until present, in Spain the major part of the meteorological observations detected in early instrumental periods were made by medical doctors, who started to pay attention to the environmental factors influencing population health under the Hippocrates oath, although also there are military institutions and academic university staff (e.g. physicists, mathematicians, etc.). Due to the high spatial and temporal climate variability in the Iberian Peninsula, it is important to recover and digitize more climatic series, and this is one of the main goals of the Salvá-Sinobas project (http://salva-sinobas.uvigo.es/) funded by the Spanish Ministry of Environment, and Rural and Marine Affairs for the 2009-2011 period. The first new series with systematic observations was detected in the city of Valencia, in the eastern façade of the Iberian Peninsula. The meteorological observations were daily published in the newspapers Diario de Valencia (1804-1834) and Diario Mercantil de Valencia (1837-1863) until official meteorological observations started in 1858 at the University of Valencia. Each day 3-daily observations (morning, midday, afternoon) were published with five climatic variables: temperature, air pressure, humidity, wind direction and the sky state. Only during the 1804-1808 period daily rainfall data is available. We checked the observer comments published in the newspapers to obtain metadata about the instruments and meteorological station information. Unfortunately, temperature data

Sodium bicarbonate induces crystalline wax generation, activates host-resistance, and increases imazalil level in rind wounds of oranges, improving the control of green mold during storage.

PubMed

Dore, Antonio; Molinu, Maria Giovanna; Venditti, Tullio; D'Hallewin, Guy

2010-06-23

Imazalil (IMZ) was quantified in the flavedo and albedo (Citrus fruits outer and inner tissue of the exocarp) of wounded and unwounded Valencia L. Olinda oranges following a 2 min immersion at 25 degrees C in 50, 100, or 250 microg mL(-1) of the fungicide mixture with or without 3% sodium bicarbonate (SBC). The addition of SBC significantly reduced the decay incidence throughout 30 d of storage at 10 degrees C with 95% RH and 6 d of simulated marketing period at 25 degrees C and 75% RH. In unwounded oranges, IMZ uptake was not changed by the coapplication of SBC, and the fungicide was predominantly recovered in the flavedo. To the contrary, in the albedo of wounded fruit, the residue level increased by about 6-fold when the fungicide was applied with SBC. When SBC was coapplied to wounded fruit, the phytoalexin scoparone was induced in the albedo and the accumulation was not affected by IMZ. When fruit was treated with SBC, scanning electron microscopy observations evidenced a production of crystalline wax patches with branched stripes and the magnitude was positively correlated to the salt concentration in the mixture. The generation as fast as 24 h post-treatment, and the different morphology of the new wax suggests a displacement of intracuticular waxes which can affect the fungicide sorption and diffusion coefficient into the rind.

Synergistic Use of SMOS Measurements with SMAP Derived and In-situ Data over Valencia Anchor Station by Using Downscaling Technique

NASA Astrophysics Data System (ADS)

Ansari Amoli, Abdolreza; Lopez-Baeza, Ernesto; Mahmoudi, Ali; Mahmoodi, Ali

2016-07-01

Synergistic Use of SMOS Measurements with SMAP Derived and In-situ Data over the Valencia Anchor Station by Using a Downscaling Technique Ansari Amoli, A.(1),Mahmoodi, A.(2) and Lopez-Baeza, E.(3) (1) Department of Earth Physics and Thermodynamics, University of Valencia, Spain (2) Centre d'Etudes Spatiales de la BIOsphère (CESBIO), France (3) Department of Earth Physics and Thermodynamics, University of Valencia, Spain Soil moisture products from active sensors are not operationally available. Passive remote sensors return more accurate estimates, but their resolution is much coarser. One solution to overcome this problem is the synergy between radar and radiometric data by using disaggregation (downscaling) techniques. Few studies have been conducted to merge high resolution radar and coarse resolution radiometer measurements in order to obtain an intermediate resolution product. In this paper we present an algorithm using combined available SMAP (Soil Moisture Active and Passive) radar and SMOS (Soil Moisture and Ocean Salinity) radiometer measurements to estimate surface soil moisture over the Valencia Anchor Station (VAS), Valencia, Spain. The goal is to combine the respective attributes of the radar and radiometer observations to estimate soil moisture at a resolution of 3 km. The algorithm disaggregates the coarse resolution SMOS (15 km) radiometer brightness temperature product based on the spatial variation of the high resolution SMAP (3 km) radar backscatter. The disaggregation of the radiometer brightness temperature uses the radar backscatter spatial patterns within the radiometer footprint that are inferred from the radar measurements. For this reason the radar measurements within the radiometer footprint are scaled by parameters that are derived from the temporal fluctuations in the radar and radiometer measurements.

Inheritance of resistance to orange rust in sugarcane

USDA-ARS?s Scientific Manuscript database

Orange rust, caused by Puccinia kuehnii, is an economically important disease in the Florida sugarcane industry. In this study, orange rust reactions of seedlings in progenies originating from 12 crosses between female and male parents with differing resistance to orange rust (three of each categor...

Inheritance of resistance to orange rust in sugarcane

USDA-ARS?s Scientific Manuscript database

Orange rust, caused by Puccinia kuehnii, is an economically important disease in the Florida sugarcane industry. In this study, orange rust reactions of seedlings in progenies originating from 12 crosses between female and male parents with differing resistance to orange rust (three of each category...

[Prevalence of exposure to occupational risks in pregnant Spanish workers (the INMA Project-Valencia)].

PubMed

González-Galarzo, M Carmen; García, Ana M; Estarlich, Marisa; García García, Francisco; Esplugues, Ana; Rodríguez, Paz; Rebagliato, Marisa; Ballester, Ferran

2009-01-01

To describe the prevalence of exposure to occupational risks among pregnant women and analyze its relationship with personal and occupational characteristics using information collected in the Childhood and Environment (Infancia y Medio Ambiente [INMA])-Valencia cohort study. The INMA-Valencia cohort study started in 2004 with 855 pregnant women living in Valencia, Spain. Data on sociodemographic variables (age, education and country of birth) and occupational conditions (activity, occupation, type of contract, working hours and self-reported occupational exposure to physical load and psychosocial, physical, chemical and biological risks) in women with paid employment during pregnancy (n=649) were collected through face-to-face interviews with a structured questionnaire in week 32 of pregnancy. The prevalences of reported exposure to physical and psychosocial load and to physical pollutants (including non-ionizing radiations) were 56%, 63% and 62%, respectively. The prevalence of reported exposure to chemicals (including cleaning products) and biological pollutants was 22% and 6%, respectively. In general, the characteristics most closely associated with exposure to occupational risks were younger age, non-Spanish nationality, lower education, having a temporary contract or being self-employed. This study is the first to quantify the prevalence of exposure to occupational risks during pregnancy in a Spanish population-based sample. According to the data observed, surveillance and control actions should be intensified in pregnant workers, as some of the observed occupational exposures have been consistently associated with detrimental reproductive and developmental effects.

How does coconut oil affect cognitive performance in alzheimer patients?

PubMed

De la Rubia Ortí, José Enrique; Sánchez Álvarez, Carmen; Selvi Sabater, Pablo; Bueno Cayo, Alma María; Sancho Castillo, Sandra; Rochina, Mariano Julián; Hu Yang, Iván

2017-03-30

Introduction: Alzheimer’s disease is one of the most prevalent neurodegenerative dementia in developed world. This fact, coupled with the lack cure, makes new no pharmacological therapeutic strategies such as nutrient management to investigate. In this regard, it stresses the possible influence of coconut oil as alternative energy source capable of stopping the progressively neuronal death that occurs in this disease. Objectives: To assess the cognitive impact of coconut oil in Alzheimer’s patients, and specifically in orientation, language-building, fixing, calculation-concentration and memory areas. Methods: Prospective, longitudinal, qualitative, analytical and experimental study through a clinical trial where 44 patients with Alzheimer’s in region of Ribera (Valencia), of which half was selected to receive during 21 days, 40 ml coconut oil daily divided between breakfast (20 ml) and food (20 ml). Before and after administration of the oil, they were evaluated through cognitive test Mini-Mental State Examination to determine possible changes. Results: It was observed in patients who received coconut oil, that cognitive improvement after completion of the intervention, statistically significant improved in the orientation and language-construction areas. Conclusions: Coconut oil appears to improve cognitive abilities of Alzheimer’s patients, with different intensity depending on the cognitive area.

Photoconversion in orange and red fluorescent proteins

PubMed Central

Kremers, Gert-Jan; Hazelwood, Kristin L.; Murphy, Christopher S.; Davidson, Michael W.; Piston, David W.

2009-01-01

We report that photoconversion is fairly common among orange and red fluorescent proteins, as a screen of 12 variants yielded 8 that exhibit photoconversion. Specifically, three red fluorescent proteins can be switched into a green state, and two orange variants can be photoconverted to the far red. The orange highlighters are ideal for dual-probe highlighter applications, and they exhibit the most red-shifted excitation of all fluorescent protein described to date. PMID:19363494

Urinary excretion of Citrus flavanones and their major catabolites after consumption of fresh oranges and pasteurized orange juice: A randomized cross-over study.

PubMed

Aschoff, Julian K; Riedl, Ken M; Cooperstone, Jessica L; Högel, Josef; Bosy-Westphal, Anja; Schwartz, Steven J; Carle, Reinhold; Schweiggert, Ralf M

2016-12-01

Orange juice contains flavanones including hesperidin and narirutin, albeit at lower concentrations as compared to orange fruit. Therefore, we compared bioavailability and colonic catabolism of flavanones from orange juice to a 2.4-fold higher dose from fresh oranges. Following a randomized two-way cross-over design, 12 healthy subjects consumed a test meal comprising either fresh oranges or pasteurized orange juice, delivering 1774 and 751 μmol of total Citrus flavanones, respectively. Deglucuronidated and desulfated hesperetin, naringenin, and the flavanone catabolites 3-(3'-hydroxy-4'-methoxyphenyl)propionic acid, 3-(3'-hydroxyphenyl)hydracrylic acid, 4-hydroxyhippuric acid, and hippuric acid were quantitated in 24-h urine by UHPLC-MS/MS. Differences in urinary hesperetin excretion were found to be nonsignificant (p = 0.5209) both after consumption of orange fruit (21.6 ± 8.0 μmol) and juice (18.3 ± 7.2 μmol). By analogy, postprandial flavanone catabolite excretions were highly similar between treatments. Excretion of 3-(3'-hydroxy-4'-methoxyphenyl)propionic acid was inversely related to that of hesperetin, illustrating the catabolite/precursor relationship. Despite 2.4-fold higher doses, excretion of flavanones from ingested fresh orange fruit did not differ from that following orange juice consumption, possibly due to a saturation of absorption or their entrapment in the fiber-rich matrix of the fruit. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Understanding the Impacts of Land-Use Policies on a Threatened Species: Is There a Future for the Bornean Orang-utan?

PubMed Central

Wich, Serge A.; Gaveau, David; Abram, Nicola; Ancrenaz, Marc; Baccini, Alessandro; Brend, Stephen; Curran, Lisa; Delgado, Roberto A.; Erman, Andi; Fredriksson, Gabriella M.; Goossens, Benoit; Husson, Simon J.; Lackman, Isabelle; Marshall, Andrew J.; Naomi, Anita; Molidena, Elis; Nardiyono; Nurcahyo, Anton; Odom, Kisar; Panda, Adventus; Purnomo; Rafiastanto, Andjar; Ratnasari, Dessy; Santana, Adi H.; Sapari, Imam; van Schaik, Carel P.; Sihite, Jamartin; Spehar, Stephanie; Santoso, Eddy; Suyoko, Amat; Tiju, Albertus; Usher, Graham; Atmoko, Sri Suci Utami; Willems, Erik P.; Meijaard, Erik

2012-01-01

The geographic distribution of Bornean orang-utans and its overlap with existing land-use categories (protected areas, logging and plantation concessions) is a necessary foundation to prioritize conservation planning. Based on an extensive orang-utan survey dataset and a number of environmental variables, we modelled an orang-utan distribution map. The modelled orang-utan distribution map covers 155,106 km2 (21% of Borneo's landmass) and reveals four distinct distribution areas. The most important environmental predictors are annual rainfall and land cover. The overlap of the orang-utan distribution with land-use categories reveals that only 22% of the distribution lies in protected areas, but that 29% lies in natural forest concessions. A further 19% and 6% occurs in largely undeveloped oil palm and tree plantation concessions, respectively. The remaining 24% of the orang-utan distribution range occurs outside of protected areas and outside of concessions. An estimated 49% of the orang-utan distribution will be lost if all forest outside of protected areas and logging concessions is lost. To avoid this potential decline plantation development in orang-utan habitats must be halted because it infringes on national laws of species protection. Further growth of the plantation sector should be achieved through increasing yields in existing plantations and expansion of new plantations into areas that have already been deforested. To reach this goal a large scale island-wide land-use masterplan is needed that clarifies which possible land uses and managements are allowed in the landscape and provides new standardized strategic conservation policies. Such a process should make much better use of non-market values of ecosystem services of forests such as water provision, flood control, carbon sequestration, and sources of livelihood for rural communities. Presently land use planning is more driven by vested interests and direct and immediate economic gains, rather than by

21 CFR 146.150 - Canned concentrated orange juice.

Code of Federal Regulations, 2014 CFR

2014-04-01

... dilution ratio greater than 3 plus 1 is “Canned concentrated orange juice, ___ plus 1” or “Canned orange juice concentrate, ___ plus 1”, the blank being filled in with the whole number showing the dilution ratio; for example, “Canned orange juice concentrate, 4 plus 1”. However, where the label bears...

21 CFR 146.150 - Canned concentrated orange juice.

Code of Federal Regulations, 2010 CFR

2010-04-01

... dilution ratio greater than 3 plus 1 is “Canned concentrated orange juice, ___ plus 1” or “Canned orange juice concentrate, ___ plus 1”, the blank being filled in with the whole number showing the dilution ratio; for example, “Canned orange juice concentrate, 4 plus 1”. However, where the label bears...

21 CFR 146.150 - Canned concentrated orange juice.

Code of Federal Regulations, 2013 CFR

2013-04-01

... dilution ratio greater than 3 plus 1 is “Canned concentrated orange juice, ___ plus 1” or “Canned orange juice concentrate, ___ plus 1”, the blank being filled in with the whole number showing the dilution ratio; for example, “Canned orange juice concentrate, 4 plus 1”. However, where the label bears...

21 CFR 146.150 - Canned concentrated orange juice.

Code of Federal Regulations, 2011 CFR

2011-04-01

... dilution ratio greater than 3 plus 1 is “Canned concentrated orange juice, ___ plus 1” or “Canned orange juice concentrate, ___ plus 1”, the blank being filled in with the whole number showing the dilution ratio; for example, “Canned orange juice concentrate, 4 plus 1”. However, where the label bears...

21 CFR 146.150 - Canned concentrated orange juice.

Code of Federal Regulations, 2012 CFR

2012-04-01

... dilution ratio greater than 3 plus 1 is “Canned concentrated orange juice, ___ plus 1” or “Canned orange juice concentrate, ___ plus 1”, the blank being filled in with the whole number showing the dilution ratio; for example, “Canned orange juice concentrate, 4 plus 1”. However, where the label bears...

Effect of chlorine dioxide gas on Salmonella enterica inoculated on navel orange surfaces and its impact on the quality attributes of treated oranges.

PubMed

Bhagat, Arpan; Mahmoud, Barakat S M; Linton, Richard H

2011-01-01

Microorganisms, including pathogens of public health significance, have been shown to contaminate orange juice during the mechanical extraction of juice. The problem gets exacerbated when washed oranges have high initial microbial load, due to an insufficient postharvest treatment. The objective of this study was to investigate the reduction of Salmonella enterica on orange surfaces using ClO₂ gas treatments to achieve a 5 log reduction, consistent with the recommendations of the U.S. Department of Agriculture-National Advisory Committee on Microbiological Criteria for Foods. A mixed culture of four Salmonella strains, isolated from previous orange juice outbreaks, was spot inoculated onto orange skin surface areas. The oranges were then treated with 0.1, 0.3, and 0.5 mg/L ClO₂ gas for 2-14 minutes at 22°C and 90%-95% relative humidity. Surviving bacteria on treated areas were recovered and enumerated over treatment time on a nonselective medium, tryptic soy agar, followed by culturing onto a selective medium, xylose lysine deoxycholate agar. A >5 log reduction of Salmonella per sample of orange surface was observed with 0.1 and 0.3 mg/L ClO₂ gas treatments at 14 minutes and a similar log reduction was observed at 0.5 mg/L ClO₂ gas at 10 minutes. This result demonstrates that the treatment of oranges with ClO₂ gas is a promising technology that could be successfully employed for the treatment of whole oranges to reduce the risk of Salmonella outbreaks in orange juice.

Relationships between nutrient composition of flowers and fruit quality in orange trees grown in calcareous soil.

PubMed

Pestana, Maribela; Beja, Pedro; Correia, Pedro José; de Varennes, Amarilis; Faria, Eugénio Araújo

2005-06-01

To determine if flower nutrient composition can be used to predict fruit quality, a field experiment was conducted over three seasons (1996-1999) in a commercial orange orchard (Citrus sinensis (L.) Osbeck cv. 'Valencia Late', budded on Troyer citrange rootstock) established on a calcareous soil in southern Portugal. Flowers were collected from 20 trees during full bloom in April and their nutrient composition determined, and fruits were harvested the following March and their quality evaluated. Patterns of covariation in flower nutrient concentrations and in fruit quality variables were evaluated by principal component analysis. Regression models relating fruit quality variables to flower nutrient composition were developed by stepwise selection procedures. The predictive power of the regression models was evaluated with an independent data set. Nutrient composition of flowers at full bloom could be used to predict the fruit quality variables fresh fruit mass and maturation index in the following year. Magnesium, Ca and Zn concentrations measured in flowers were related to fruit fresh mass estimations and N, P, Mg and Fe concentrations were related to fruit maturation index. We also established reference values for the nutrient composition of flowers based on measurements made in trees that produced large (> 76 mm in diameter) fruit.

Developing a MOOC Initiative: Lessons Learnt from the Universitat Politecnica de Valencia Experience

ERIC Educational Resources Information Center

Despujol, Ignacio; Castaneda, Linda; Turro, Carlos

2018-01-01

Universitat Politecnica de Valencia (UPV) was the European institution with more MOOC course runs done by December 2016. This paper describes and analyses the decisionmaking process, and rationale of the development of this low budget real MOOC institutional initiative carried out by a traditional higher educational medium sized institution. This…

Health effects of the 2012 Valencia (Spain) wildfires on children in a cohort study.

PubMed

Vicedo-Cabrera, Ana M; Esplugues, Ana; Iñíguez, Carmen; Estarlich, Marisa; Ballester, Ferran

2016-06-01

In July 2012, two simultaneous wildfires burnt a big area in Valencia (Spain), where a birth cohort study (INMA) is being developed. The heavy smoke covered the whole INMA study area for several days. We aimed at evaluating the 2012 Valencia wildfire effects on the health of children enrolled in the INMA-Valencia cohort. Two weeks after the extinction of the wildfires, a phone survey was conducted and finally 460 individuals were enrolled. We considered a wildfire period (12-day interval when they were active) and a control period (12-day interval just before wildfires). Parents were asked about respiratory symptoms experienced during both periods, and during wildfires only about the preventive measures adopted and the perception of exposure, along with individual data collected through the different follow-up surveys of the cohort. Conditional logistic regression models were applied, and we included interaction terms for asthma/rhinitis and level of perception of exposure; 82.4 % perceived smoke smell outdoors, 40 % indoors and more than 90 % of the families observed the presence of ash. An adjusted odds ratio of 3.11 [95 % confidence interval 1.62-5.97] for itchy/watery eyes and 3.02 [1.41-6.44] for sore throat was obtained. Significant interaction terms for rhinitis and asthma in itchy/watery eyes and sneezing, and only asthma for sore throat were obtained. Exposure to wildfire smoke was associated with increased respiratory symptoms in this child population, particularly affecting susceptible individuals with asthma or rhinitis.

7 CFR 29.1043 - Orange (F).

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 2 2010-01-01 2010-01-01 false Orange (F). 29.1043 Section 29.1043 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... Type 92) § 29.1043 Orange (F). A reddish yellow. [42 FR 21092, Apr. 25, 1977. Redesignated at 47 FR...

Isolation and Screening of Pectinolytic Fungi from Orange (Citrus nobilis Tan.) and Banana (Musa acuminata L.) Fruit Peel

NASA Astrophysics Data System (ADS)

Amilia, K. R.; Sari, S. L. A.; Setyaningsih, R.

2017-04-01

Pectinase is the one of most important enzyme which is used in food industry such as fruit and vegetable juice extraction, oil extraction and fermentation of coffee, cocoa and tea. Pectinase can be produced by microorganism such as bacteria and fungi. Fungi are known as potent producer of pectinase. This research was conducted to isolate and screen of the pectinolytic fungi from rotten orange and banana fruit peels. This research succeeded to isolate 10 fungal isolates from rotten orange peels and 5 fungal isolates from rotten banana peels. These isolates were screened in pectinolytic activities based on clear zone formation on pectic medium which is stained by cetyl trimethyl ammonium bromide. The screening result showed that fungal isolates which showed pectinolytic activity were O2, O3, O4, O7, O8, O10, B3, and B5. Based on morphological characters, pectinolytic fungi were identified as Fusarium O4 and O10, Penicillium O2, Aspergillus O3, O7, B3 and B5 and Trichoderma O8. The highest pectinolytic activity was showed by Penicillium O2 which was isolated from orange peel.

Inhibitory effects of some plant essential oils against Arcobacter butzleri and potential for rosemary oil as a natural food preservative.

PubMed

Irkin, Reyhan; Abay, Secil; Aydin, Fuat

2011-03-01

We investigated the inhibitory activity of commercially marketed essential oils of mint, rosemary, orange, sage, cinnamon, bay, clove, and cumin against Arcobacter butzleri and Arcobacter skirrowii and the effects of the essential oil of rosemary against A. butzleri in a cooked minced beef system. Using the disc diffusion method to determine the inhibitory activities of these plant essential oils against strains of Arcobacter, we found that those of rosemary, bay, cinnamon, and clove had strong inhibitory activity against these organisms, whereas the essential oils of cumin, mint, and sage failed to show inhibitory activity against most of the Arcobacter strains tested. The 0.5% (vol/wt) essential oil of rosemary was completely inhibitory against A. butzleri in the cooked minced beef system at 4°C. These essential oils may be further investigated as a natural solution to the food industry by creating an additional barrier (hurdle technology) to inhibit the growth of Arcobacter strains.

Shelter and remotely sensed night temperatures in orange groves

NASA Astrophysics Data System (ADS)

Caselles, V.; Sobrino, J. A.

1991-06-01

In previous papers we have used a linear regression approach for determining nocturnal air temperature in orange groves from satellite thermal data. However, this procedure has a poor precision (≈ 2 °C) for applications such as frost forecasting. For this reason a theoretical method has been proposed, which is based on the following assumptions: (1) the air temperature ( T a) is the result of the convective heat exchange between ground and air, and between air and orange trees, and (2) the remotely-sensed temperature ( T) can be expressed as a function of ground ( T g) and orange tree ( T s) temperatures. So the relationship T = T a + ( a g - α) ( T g - Ts) has been derived, where a = (1 + h 2πR/h1L)-1 and α g = ( ɛ g/ɛ) [ P g + (1 - ɛ 0) G'P s]; h 1 is the convective heat transfer coefficient between ground and air, h 2 is the convective heat transfer coefficient between air and orange tree, R is the orange tree radius, L is the distance between two orange tree trunks, ɛ g and ɛ 0 are the emissivities of the ground and of the orange tree, ɛ is the effective emissivity, P g and P s are the proportions of ground and side of the orange tree observed by the sensor, and G' is the shape factor ground-side. Two experiments were carried out in order to validate this model, in which air temperature was measured by means of a mercury thermometer positioned at 1.5m above the ground and in the middle of two orange tree rows. The temperature of the orange tree and the ground was measured with a radiometer, and the temperature of the orange grove was obtained by means of a manual scanning system. Ground and orange tree emissivities were measured using the box method. We have analyzed the dependence of the T-T a relationship on weather conditions, field architecture and viewing angle, and we can conclude that if these parameters are known, the shelter temperature can be obtained from satellite thermal data with a precision of 0.8 °C.

Orange County Photovoltaic Project & Educational COmponent

DOE Office of Scientific and Technical Information (OSTI.GOV)

Parker, Renee

The purpose of this report is to discuss the projects implemented, utilizing Department of Energy grant funds, to support the use and understanding of renewable energy in Orange County, Florida and the Greater Orlando Area. Orange County is located in the State of Florida and is most popularly referred to as Orlando. The greater Orlando area’s current population is 1,225,267 and in 2015 was the first destination to surpass 60 million visitors. Orange County utilized grant funds to add to the growing demand for access to charging stations by installing one level 2 dual NovaCharge CT4021 electric vehicle charging stationmore » at the Orange County/University of Florida Cooperative Extension Center. The charging station is considered a “smart” charger connected to a central network operated by a third party. Data collected includes the number of charging sessions, session start and end times, the electricity usage, greenhouse gases saved and other pertinent data used for reporting purposes. Orange County continues to support the use of electric vehicles in Metro Orlando and this project continues to bring awareness to our public regarding using alternative vehicles. Additionally, we offer all visitors to the Orange County/University of Florida Cooperative Extension Center free charges for their electric vehicles 24 hours a day. Since the operation of the charging station there have been 52 unique driver users, a total of 532.2258 kg of greenhouse gas savings and 159.03 gallons of gasoline savings. The installation of the additional electric vehicle charging station is part of a county-wide goal of promoting implementation of renewable energy technologies as well as supporting the use of electric vehicles including the Drive Electric Orlando & Florida programs. http://driveelectricorlando.com/ & ; http://www.driveelectricflorida.org/ . Grant funds were also used for Outreach and Educational efforts. Educational efforts about renewable energy were accomplished

Palaeogeographic implications of the Messinian surface in the Valencia trough, northwestern Mediterranean Sea

NASA Astrophysics Data System (ADS)

Escutia, C.; Maldonado, A.

1992-03-01

Sparker (3000 J and 8000 J) and multichannel seismic reflection profiles across the Valencia trough show a Messinian unconformity incised by numerous valleys. The main feature of this surface is a large valley that generally underlies the present Valencia valley and is deeply entrenched into the Miocene deposits. The size of this palaeo-valley ranges from 0.5 km wide and 15-100 m deep at its western end, to 1.6-2.8 km wide and 200-250 m deep downstream. An important tributary system is observed, with a main canyon (6-8 km wide and 150-200 m deep) draining the Ebro margin, as well as many other smaller valleys draining the Catalan and Balearic margins. Downstream, other tributaries underlie the present canyons of the Catalan margin. The location of the tributary system is controlled by the Early Miocene rift structures. The relief of the Messinian surface is affected by post-Miocene deformation that results from salt diapirism, extensional faulting and related volcanism. Late Neogene to Quaternary volcanic edifices cut the Messinian surface and coincide with large residual magnetic anomalies. Lower Pliocene to Quaternary salt diapirism in the abyssal plain north of Menorca has created a series of structural highs. Between these highs are deep interdiapiric troughs or basins that have become sediment depocentres during the Plio-Quaternary. The complex network of erosional valleys from the Valencia trough continental margin demonstrates that the valley system in the basin was not related to the refilling of the Mediterranean, but to the Iberian and Balearic margin palaeodrainage that developed during the Messinian desiccation. The presence of at least three erosional unconformities suggests that there were alternating periods of flooding and retreat of Atlantic water during Messinian time. The Messinian subaerial margin with erosional valleys contrasts sharply with the Pliocene-Quaternary marine margin with progradational turbidite systems.

Model Uncertainties for Valencia RPA Effect for MINERvA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gran, Richard

2017-05-08

This technical note describes the application of the Valencia RPA multi-nucleon effect and its uncertainty to QE reactions from the GENIE neutrino event generator. The analysis of MINERvA neutrino data in Rodrigues et al. PRL 116 071802 (2016) paper makes clear the need for an RPA suppression, especially at very low momentum and energy transfer. That published analysis does not constrain the magnitude of the effect; it only tests models with and without the effect against the data. Other MINERvA analyses need an expression of the model uncertainty in the RPA effect. A well-described uncertainty can be used for systematics for unfolding, for model errors in the analysis of non-QE samples, and as input for fitting exercises for model testing or constraining backgrounds. This prescription takes uncertainties on the parameters in the Valencia RPA model and adds a (not-as-tight) constraint from muon capture data. For MINERvA we apply it as a 2D (more » $$q_0$$,$$q_3$$) weight to GENIE events, in lieu of generating a full beyond-Fermi-gas quasielastic events. Because it is a weight, it can be applied to the generated and fully Geant4 simulated events used in analysis without a special GENIE sample. For some limited uses, it could be cast as a 1D $Q^2$ weight without much trouble. This procedure is a suitable starting point for NOvA and DUNE where the energy dependence is modest, but probably not adequate for T2K or MicroBooNE.« less

Osage orange (Maclura pomifera L) seed oil poly-(-a-hydroxy dibutylamine) triglycerides: Synthesis and characterization

USDA-ARS?s Scientific Manuscript database

In exploring alternative vegetable oils for non-food industrial applications, especially in temperate climates, tree seed oils that are not commonly seen as competitors to soybean, peanut, and corn oils can become valuable sources of new oils. Many trees produce edible fruits and seeds while others ...

Light pollution in Valencia: public awareness

NASA Astrophysics Data System (ADS)

Marco, E.; Morales-Rubio, A.

2015-05-01

Light pollution is caused by the direct and indirect light emission into the atmosphere from artificial night sources. The most decisive effect is the increasing of the luminance of the sky preventing stargazing. The fundamental actor for solving the problem is public awareness. Therefore, in accordance with authorities of the Valencian natural parks, municipalities and high schools, we have held a series of explanatory talks to the general public on the problem of light pollution, not only about the loss of the night and its impact on Astronomy, but also about the waste of energy and its impact on nocturnal wildlife and human health. Furthermore, in order to emphasize the need to preserve dark skies, popular astronomical observations have been also performed. An informative brochure on the problem of light pollution, with real cases involving natural parks, Valencia and its metropolitan area, has been published.

Lippia origanoides essential oil: an efficient and safe alternative to preserve food, cosmetic and pharmaceutical products.

PubMed

Hernandes, C; Pina, E S; Taleb-Contini, S H; Bertoni, B W; Cestari, I M; Espanha, L G; Varanda, E A; Camilo, K F B; Martinez, E Z; França, S C; Pereira, A M S

2017-04-01

The aim of this work was to evaluate the efficacy and safety of Lippia origanoides essential oil as a preservative in industrial products. The composition, antimicrobial activity, mutagenic and toxic potential of L. origanoides were determined. Then, the effect of essential oil as a preservative in food, cosmetics and pharmaceutical products was evaluated. The essential oil of L. origanoides consisted mainly of oxygenated monoterpenes (38·13%); 26·28% corresponded to the compound carvacrol. At concentrations ranging from 0·312 to 1·25 μl ml -1 and in association with polysorbate 80, the essential oil of L. origanoides inhibited the growth of all the tested micro-organisms. The medium lethal dose in mice was 3·5 g kg -1 , which categorizes it as nontoxic according to the European Union criteria, and negative results in the Ames test indicated that this oil was not mutagenic. In combination with polysorbate 80, the essential oil exerted preservative action on orange juice, cosmetic and pharmaceutical compositions, especially in the case of aqueous-based products. Lippia origanoides essential oil is an effective and safe preservative for orange juice, pharmaceutical and cosmetic products. This study allowed for the complete understanding of the antimicrobial action and toxicological potential of L. origanoides essential oil. These results facilitate the development of a preservative system based on L. origanoides essential oil. © 2017 The Society for Applied Microbiology.

ORANGES evaluation final report : for the US DOT sponsored evaluation of the ORANGES electronic payment systems field operational test

DOT National Transportation Integrated Search

2004-12-06

This report describes the findings of the US DOT-sponsored evaluation of the Orlando (Florida) ORANGES multi-modal Field Operational Test (FOT); the report includes: a background description of the ORANGES FOT; the Evaluation Strategy and Plan, which...

The draft genome of sweet orange (Citrus sinensis).

PubMed

Xu, Qiang; Chen, Ling-Ling; Ruan, Xiaoan; Chen, Dijun; Zhu, Andan; Chen, Chunli; Bertrand, Denis; Jiao, Wen-Biao; Hao, Bao-Hai; Lyon, Matthew P; Chen, Jiongjiong; Gao, Song; Xing, Feng; Lan, Hong; Chang, Ji-Wei; Ge, Xianhong; Lei, Yang; Hu, Qun; Miao, Yin; Wang, Lun; Xiao, Shixin; Biswas, Manosh Kumar; Zeng, Wenfang; Guo, Fei; Cao, Hongbo; Yang, Xiaoming; Xu, Xi-Wen; Cheng, Yun-Jiang; Xu, Juan; Liu, Ji-Hong; Luo, Oscar Junhong; Tang, Zhonghui; Guo, Wen-Wu; Kuang, Hanhui; Zhang, Hong-Yu; Roose, Mikeal L; Nagarajan, Niranjan; Deng, Xiu-Xin; Ruan, Yijun

2013-01-01

Oranges are an important nutritional source for human health and have immense economic value. Here we present a comprehensive analysis of the draft genome of sweet orange (Citrus sinensis). The assembled sequence covers 87.3% of the estimated orange genome, which is relatively compact, as 20% is composed of repetitive elements. We predicted 29,445 protein-coding genes, half of which are in the heterozygous state. With additional sequencing of two more citrus species and comparative analyses of seven citrus genomes, we present evidence to suggest that sweet orange originated from a backcross hybrid between pummelo and mandarin. Focused analysis on genes involved in vitamin C metabolism showed that GalUR, encoding the rate-limiting enzyme of the galacturonate pathway, is significantly upregulated in orange fruit, and the recent expansion of this gene family may provide a genomic basis. This draft genome represents a valuable resource for understanding and improving many important citrus traits in the future.

8. August, 1970 PUMP BEHIND PELEG COGGESHALL HOUSE, 10 ORANGE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. August, 1970 PUMP BEHIND PELEG COGGESHALL HOUSE, 10 ORANGE STREET (MASS-1063) - Orange & Union Streets Neighborhood Study, 8-31 Orange Street, 9-21 Union Street & Stone Alley, Nantucket, Nantucket County, MA

Orange County Government Solar Demonstration and Research Facility

DOE Office of Scientific and Technical Information (OSTI.GOV)

Parker, Renee; Cunniff, Lori

Orange County Florida completed the construction of a 20 kilowatt Solar Demonstration and Research Facility in March 2015. The system was constructed at the Orange County/University of Florida Cooperative Extension Center whose electric service address is 6021 South Conway Road, Orlando, Florida 32802. The Solar Demonstration and Research Facility is comprised of 72 polycrystalline photovoltaic modules and 3 inverters which convert direct current from the solar panels to alternating current electricity. Each module produces 270 watts of direct current power, for a total canopy production of just under 20,000 watts. The solar modules were installed with a fixed tilt ofmore » 5 degrees and face south, toward the equator to maximize the amount of sunlight captures. Each year, the electricity generated by the solar array will help eliminate 20 metric tons of carbon dioxide emissions as well as provide covered parking for staff and visitors vehicles. The solar array is expected to generate 27,000 kilowatt hours of electricity annually equating to an estimated $266 savings in the monthly electric bill, or $3,180 annually for the Orange County/University of Florida Cooperative Extension Center. In addition to reducing the electric bill for the Extension Center, Orange County’s solar array also takes advantage of a rebate incentive offered by the local utility, Orlando Utility Commission, which provided a meter that measures the amount of power produced by the solar array. The local utility company’s Solar Photovoltaic Production Incentive will pay Orange County $0.05 per kilowatt hour for the power that is produced by the solar array. This incentive is provided in addition to Net Metering benefits, which is an effort to promote the use of clean, renewable energy on the electric grid. The Photovoltaic Solar Demonstration and Research Facility also serves an educational tool to the public; the solar array is tied directly into a data logger that provides real time

Sustainability analysis using FORSEE and continuous forest inventory information to compare volume estimation methods for the Valencia coast redwood tract in Santa Cruz County, California

Treesearch

Douglas D. Piirto; Mitchell Haydon; Steve Auten; Benjamin Han; Samantha Gill; Wally Mark; Dale Holderman

2017-01-01

The 1,295 ha (3,200 ac) Swanton Pacific Ranch (Swanton) and the associated Valencia Tract in Santa Cruz County have been managed by California Polytechnic State University, San Luis Obispo (Cal Poly) since 1987. Swanton’s Valencia Tract is a 239 ha (591 ac) property located north of Watsonville, California. Cal Poly forest managers have conducted two harvest...

21 CFR 82.1261 - D&C Orange No. 11.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 1 2010-04-01 2010-04-01 false D&C Orange No. 11. 82.1261 Section 82.1261 Food... CERTIFIED PROVISIONALLY LISTED COLORS AND SPECIFICATIONS Drugs and Cosmetics § 82.1261 D&C Orange No. 11. The color additive D&C Orange No. 11 shall conform in identity and specifications to the requirements...

21 CFR 82.1260 - D&C Orange No. 10.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 1 2010-04-01 2010-04-01 false D&C Orange No. 10. 82.1260 Section 82.1260 Food... CERTIFIED PROVISIONALLY LISTED COLORS AND SPECIFICATIONS Drugs and Cosmetics § 82.1260 D&C Orange No. 10. The color additive D&C Orange No. 10 shall conform in identity and specifications to the requirements...

PHOTOMICROGRAPH - SPHERE FRAGMENTS - "ORANGE" SOIL - APOLLO 17 - MSC

NASA Image and Video Library

1973-01-04

S73-15171 (4 Jan. 1973) --- These orange glass spheres and fragments are the finest particles ever brought back from the moon. Ranging in size from 20 to 45 microns (about 1/1000 of an inch) the particles are magnified 160 times in this photomicrograph made in the Lunar Receiving Laboratory at the Manned Spacecraft Center. The orange soil was brought back from the Taurus-Littrow landing site by the Apollo 17 crewmen. Scientist-astronaut Harrison H. "Jack" Schmitt discovered the orange soil at Shorty Crater during the second Apollo 17 extravehicular activity (EVA). This lunar material is being studied and analyzed by scientists in the LRL. The orange particles in this photomicrograph, which are intermixed with black and black-speckled grains, are about the same size as the particles that compose silt on Earth. Chemical analysis of the orange soil material has shown the sample to be similar to some of the samples brought back from the Apollo 11 (Sea of Tranquility) site several hundred miles to the southwest. Like those samples, it is rich in titanium (8%) and iron oxide (22%). But unlike the Apollo 11 samples, the orange soil is unexplainably rich in zinc ? an anomaly that has scientists in a quandary. This Apollo 17 sample is not high in volatile elements, nor do the minerals contain substantial amounts of water. These would have provided strong evidence of volcanic activity. On the other hand, the lack of agglutinates (rocks made up of a variety of minerals cemented together) indicates that the orange glass is probably not the product of meteorite impact -- strengthening the argument that the glass was produced by volcanic activity.

SU-F-T-23: Correspondence Factor Correction Coefficient for Commissioning of Leipzig and Valencia Applicators with the Standard Imaging IVB 1000

DOE Office of Scientific and Technical Information (OSTI.GOV)

Donaghue, J; Gajdos, S

Purpose: To determine the correction factor of the correspondence factor for the Standard Imaging IVB 1000 well chamber for commissioning of Elekta’s Leipzig and Valencia skin applicators. Methods: The Leipzig and Valencia applicators are designed to treat small skin lesions by collimating irradiation to the treatment area. Published output factors are used to calculate dose rates for clinical treatments. To validate onsite applicators, a correspondence factor (CFrev) is measured and compared to published values. The published CFrev is based on well chamber model SI HDR 1000 Plus. The CFrev is determined by correlating raw values of the source calibration setupmore » (Rcal,raw) and values taken when each applicator is mounted on the same well chamber with an adapter (Rapp,raw). The CFrev is calculated by using the equation CFrev =Rapp,raw/Rcal,raw. The CFrev was measured for each applicator in both the SI HDR 1000 Plus and the SI IVB 1000. A correction factor, CFIVB for the SI IVB 1000 was determined by finding the ratio of CFrev (SI IVB 1000) and CFrev (SI HDR 1000 Plus). Results: The average correction factors at dwell position 1121 were found to be 1.073, 1.039, 1.209, 1.091, and 1.058 for the Valencia V2, Valencia V3, Leipzig H1, Leipzig H2, and Leipzig H3 respectively. There were no significant variations in the correction factor for dwell positions 1119 through 1121. Conclusion: By using the appropriate correction factor, the correspondence factors for the Leipzig and Valencia surface applicators can be validated with the Standard Imaging IVB 1000. This allows users to correlate their measurements with the Standard Imaging IVB 1000 to the published data. The correction factor is included in the equation for the CFrev as follows: CFrev= Rapp,raw/(CFIVB*Rcal,raw). Each individual applicator has its own correction factor, so care must be taken that the appropriate factor is used.« less

ORANGES evaluation final report appendices : for the US DOT sponsored evaluation of the ORANGES electronic payment systems field operational test

DOT National Transportation Integrated Search

2004-12-06

These are the appendices for the report describing the findings of the US DOT-sponsored evaluation of the Orlando (Florida) ORANGES multi-modal Field Operational Test (FOT); the report includes: a background description of the ORANGES FOT; the Evalua...

21 CFR 74.1255 - D&C Orange No. 5.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 1 2010-04-01 2010-04-01 false D&C Orange No. 5. 74.1255 Section 74.1255 Food and... ADDITIVES SUBJECT TO CERTIFICATION Drugs § 74.1255 D&C Orange No. 5. (a) Identity. (1) the color additive D&C Orange No. 5 is a mixture consisting principally the sodium salt of 4′,5′-dibromofluorescein (CAS...

21 CFR 74.1261 - D&C Orange No. 11.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 1 2010-04-01 2010-04-01 false D&C Orange No. 11. 74.1261 Section 74.1261 Food... COLOR ADDITIVES SUBJECT TO CERTIFICATION Drugs § 74.1261 D&C Orange No. 11. (a) Identity. (1) The color additive D&C Orange No. 11 is a mixture consisting principally of the disodium salts of 4′,5...

21 CFR 74.1254 - D&C Orange No. 4.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 1 2010-04-01 2010-04-01 false D&C Orange No. 4. 74.1254 Section 74.1254 Food and... ADDITIVES SUBJECT TO CERTIFICATION Drugs § 74.1254 D&C Orange No. 4. (a) Identity. (1) the color additive D&C Orange No. 4 is principally the sodium salt of 4-[(2-hydroxy-1-naphthalenyl)azo]benzenesulfonic...

21 CFR 82.1254 - D&C Orange No. 4.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 1 2010-04-01 2010-04-01 false D&C Orange No. 4. 82.1254 Section 82.1254 Food and... PROVISIONALLY LISTED COLORS AND SPECIFICATIONS Drugs and Cosmetics § 82.1254 D&C Orange No. 4. The color additive D&C Orange No. 4 shall conform in identity and specifications to the requirements of § 74.1254(a...

The effect of lemon, orange and bergamot essential oils and their components on the survival of Campylobacter jejuni, Escherichia coli O157, Listeria monocytogenes, Bacillus cereus and Staphylococcus aureus in vitro and in food systems.

PubMed

Fisher, K; Phillips, C A

2006-12-01

To investigate the effectiveness of oils and vapours of lemon (Citrus limon), sweet orange (Citrus sinensis) and bergamot (Citrus bergamia) and their components against a number of common foodborne pathogens. The disc diffusion method was used to screen the oils and vapours against Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Escherichia coli O157 and Campylobacter jejuni. The survival of each species, demonstrated to be susceptible in the in vitro studies, was tested on cabbage leaf for 60 s by direct contact and on chicken skin for 10 min by direct contact and 24 h by vapour. The results indicate that bergamot was the most inhibitory essential oil (EO) and citral and linalool mimicked its effect (P > 0.001). Citral and linalool vapours produced 6 log reductions in L. monocytogenes, Staph. aureus and B. cereus populations on cabbage leaf after 8-10 h exposure but bergamot vapour exposure, while producing a similar reduction in L. monocytogenes and B. cereus populations, had no effect on Staph. aureus. Bergamot was the most effective of the oils tested and linalool the most effective anti-bacterial component. Gram-positive bacteria were more susceptible than Gram-negative bacteria in vitro, although Camp. jejuni and E. coli O157 were inhibited by bergamot and linalool oils and by linalool vapour. All bacteria tested were less susceptible in food systems than in vitro. Of the Gram-positive bacteria tested Staph. aureus was the least susceptible to both the oils and the components tested. Results suggest the possibility that citrus EOs, particularly bergamot, could be used as a way of combating the growth of common causes of food poisoning.

The Tethys Rifting of the Valencia Trough Basin

NASA Astrophysics Data System (ADS)

Viñas, Marina; Ranero, César R.; Cameselle, Alejandra L.

2017-04-01

The western Mediterranean submarine realm is composed of several basin inferred to be formed by a common geodynamic process: upper plate extension during slab rollback of a retreating subduction zone. Although the time evolution of the geometry of the trenches is debated, all models assume that basins opened sequentially from NW (Gulf of Lions) towards the SE (Ligurian-Provençal and later Tyrrhenian basins) and SW (Valencia Trough and later Algerian-South Balearic and Alboran Basin) as trenches migrated. Basin opening history is key to reconstruct kinematics of slab retreat preferred in each model. However, the deep structure of basins is inadequately known due to the paucity of modern wide-angle and multichannel reflection seismic studies across entire systems, and absence of deep drilling in the deep-water regions of the basins, as a result, much of the opening evolution is inferred from indirect evidence. In the Valencia Trough Basin (VTB), drilling and vintage seismic data provide good knowledge of the shallow geology of the basin. However, crustal-scale information across the entire VTB has been limited to two studies (Figure 1): One in the late 80's (Valsis experiment) with three Expanded Spread Profiles that yielded local 1D velocity/depth models used to constrain 2D gravity modeling, and a few multichannel seismic profiles along the Iberian shelf and across segments of the basin. A second study in the early 90's (ESCI experiment) collected a low-resolution deep-penetration multichannel seismic reflection profile across the basin and a coincident wide-angle seismic line with numerous land stations in Iberia but a handful of widely-spaced Ocean Bottom Seismometers. In the absence of modern detailed crustal structure, the origin and evolution of the VTB is still debated. Industry multichannel seismic reflection profiles cover the SW segment of the VTB. This is a region where the basin sea floor is comparatively shallower and has numerous industry wells

[Drugs use in pregnancy in the Valencia Region and the risk of congenital anomalies].

PubMed

Cavero-Carbonell, Clara; Gimeno-Martos, Silvia; Páramo-Rodríguez, Lucía; Rabanaque-Hernández, María José; Martos-Jiménez, Carmen; Zurriaga, Óscar

2017-09-01

Despite the potential risks of drug use during pregnancy, consumption has increased in recent decades. To identify the risk of congenital anomalies (CA) associated with the use of drugs in primary care in pregnant women residents in the Valencia Region. A case-control study, considering a case as a less than one year old live birth in 2009-2010, diagnosed with a CA and resident in the Valencia Region, obtained from the CA population-based registry. Controls were selected from the Metabolic Disease Registry, and the drugs prescribed and dispensed from the Integral Management of Pharmaceutical Services. Crude odds ratio (OR) was calculated with its 95% confidence intervals and adjusted OR was calculated using logistic regression. A total of 1,913 cases and 3,826 controls were identified. The most frequently used drug groups were those acting on the musculoskeletal, nervous and respiratory systems, on the blood and blood forming organs, and anti-infection drugs. The most common drugs used were ibuprofen, dexketoprofen, paracetamol, amoxicillin, ferrous sulphate, and a combination of folic acid. A significantly increased risk of CA was identified for drugs acting on the musculoskeletal system (adjusted OR 1.14 [95% confidence interval 1.02-1.28]). A significantly decreased risk was observed for drugs acting on the blood and blood forming organs (adjusted OR 0.87 [95% confidence interval 0.78-0.98]). Associations between drugs and CA in pregnant women resident in the Valencia Region have been identified for drugs that act as risk factors of CA, and for drugs that act as protective factors of CA. Copyright © 2016 Asociación Española de Pediatría. Publicado por Elsevier España, S.L.U. All rights reserved.

Orange peel products can reduce Salmonella populations in ruminants.

PubMed

Callaway, Todd R; Carroll, Jeffery A; Arthington, John D; Edrington, Tom S; Anderson, Robin C; Rossman, Michelle L; Carr, Mandy A; Genovese, Ken J; Ricke, Steve C; Crandall, Phil; Nisbet, David J

2011-10-01

Salmonella can live undetected in the gut of food animals and be transmitted to humans. Animal diets can impact intestinal populations of foodborne pathogens, including Salmonella spp. Orange juice production results in a waste product, orange peel and orange pulp, which has a high nutritive value and is often included in cattle diets as a least-cost ration ingredient. Here we show that the inclusion of orange peel products reduced Salmonella Typhimurium populations in the gut of experimentally inoculated sheep. Sheep (n=24) were fed a cracked corn grain-based high grain diet that was supplemented with a 50%/50% (dry matter [DM], w/w) mixture of dried orange pellet and fresh orange peel to achieve a final concentration (DM, basis) of 0%, 10%, or 20% orange product (OP) for 10 days before inoculation with Salmonella Typhimurium. Sheep were experimentally inoculated with 10(10) colony forming units Salmonella Typhimurium, and fecal samples were collected every 24 h after inoculation. Sheep were humanely euthanized at 96 h after oral Salmonella inoculation. Populations of inoculated Salmonella Typhimurium were numerically reduced by OP treatment throughout the gastrointestinal tract, and this reduction only reached significant levels in the cecum (p<0.05) of sheep fed 10% OP diets. Apparent palatability issues decreased the consumption of OP in sheep fed 20% OP to intake levels below that of 10% OP (approximately 7% dry matter intake [DMI]/d feed refusal), thereby reducing the potential effects of OP feeding at this higher level. Our results demonstrate that orange peel and pellets are environmentally friendly and low-cost products that can be used as a pre-harvest intervention as part of an integrated pathogen reduction scheme.

7 CFR 29.1044 - Orange Red (FR).

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 2 2010-01-01 2010-01-01 false Orange Red (FR). 29.1044 Section 29.1044 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... Type 92) § 29.1044 Orange Red (FR). A yellowish red. [42 FR 21092, Apr. 25, 1977. Redesignated at 47 FR...

Volatile and nonvolatile flavor chemical evaluation of USDA orange-mandarin hybrids for comparison to sweet orange and mandarin fruit

USDA-ARS?s Scientific Manuscript database

Three citrus hybrids, containing 50-75% sweet orange (Citrus sinensis) genome in their pedigrees and similar to sweet orange in fruit size, color and taste, were tested for their potential to be classified as new “sweet orange” cultivars. 'Hamlin', ‘Midsweet’, and three other early to mid-season swe...

21 CFR 74.1260 - D&C Orange No. 10.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 1 2010-04-01 2010-04-01 false D&C Orange No. 10. 74.1260 Section 74.1260 Food... COLOR ADDITIVES SUBJECT TO CERTIFICATION Drugs § 74.1260 D&C Orange No. 10. (a) Identity. (1) The color additive D&C Orange No. 10 is a mixture consisting principally of 4′,5′-diiodofluorescein, 2′,4′,5...

7 CFR 905.306 - Orange, Grapefruit, Tangerine and Tangelo Regulation.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Orange, Grapefruit, Tangerine and Tangelo Regulation... AGRICULTURE ORANGES, GRAPEFRUIT, TANGERINES, AND TANGELOS GROWN IN FLORIDA Grade and Size Requirements § 905.306 Orange, Grapefruit, Tangerine and Tangelo Regulation. (a) During the period specified in column (2...

Studies on piston bowl geometries using single blend ratio of various non-edible oils.

PubMed

Viswanathan, Karthickeyan; Pasupathy, Balamurugan

2017-07-01

The depletion of fossil fuels and hike in crude oil prices were some of the main reasons to explore new alternatives from renewable source of energy. This work presents the impact of various bowl geometries on diesel engine with diesel and biodiesel samples. Three non-edible oils were selected, namely pumpkin seed oil, orange oil and neem oil. These oils were converted into respective biodiesel using transesterification process in the presence of catalyst and alcohol. After transesterification process, the oils were termed as pumpkin seed oil methyl ester (PSOME), orange oil methyl ester (OME) and neem oil methyl ester (NOME), respectively. The engine used for experimentation was a single-cylinder four-stroke water-cooled direct-injection diesel engine and loads were applied to the engine using eddy current dynamometer. Two bowl geometries were developed, namely toroidal combustion chamber (TCC) and trapezoidal combustion chamber (TRCC). Also, the engine was inbuilt with hemispherical combustion chamber (HCC). The base line readings were recorded using neat diesel fuel with HCC for various loads. Followed by 20% of biodiesel mixed with 80% neat diesel for all prepared methyl esters and termed as B1 (20% PSOME with 80% diesel), B2 (20% OME with 80% diesel) and B3 (20% NOME with 80% diesel). All fuel samples were tested in HCC, TCC and TRCC bowl geometries under standard injection timing and with compression ratio of 18. Increased brake thermal efficiency and reduced brake specific fuel consumption were observed with diesel in TCC geometry. Also, higher heat release and cylinder pressures with lower ignition delay were recorded with TCC bowl geometry. TCC bowl geometry showed lower CO, HC and smoke emissions with B2 fuel sample than diesel and other biodiesel samples. But, higher NOx emission was observed in HCC and TCC than that in TRCC bowl geometry. Graphical abstract ᅟ.

Accidental bullous phototoxic reactions to bergamot aromatherapy oil.

PubMed

Kaddu, S; Kerl, H; Wolf, P

2001-09-01

Oil of bergamot is an extract from the rind of bergamot orange (Citrus aurantium ssp bergamia) that has a pleasant, refreshing scent; until a few years ago it had been widely used as an ingredient in cosmetics but was restricted or banned in most countries because of certain adverse effects. More recently, oil of bergamot preparations have been gaining renewed popularity in aromatherapy. Oil of bergamot possesses photosensitive and melanogenic properties because of the presence of furocoumarins, primarily bergapten (5-methoxypsoralen [5-MOP]). However, 5-MOP is also potentially phototoxic and photomutagenic. Despite its increasing application, there are only a few recent reports of phototoxic reactions to bergamot aromatherapy oil. We describe two patients with localized and disseminated bullous phototoxic skin reactions developing within 48 to 72 hours after exposure to bergamot aromatherapy oil and subsequent ultraviolet exposure. One patient (case 2) had no history of direct contact with aromatherapy oil but developed bullous skin lesions after exposure to aerosolized (evaporated) aromatherapy oil in a sauna and subsequent UVA radiation in a tanning salon. This report highlights the potential health hazard related to the increasing use of psoralen-containing aromatherapy oils.

Orange fiber laser for ophthalmology

NASA Astrophysics Data System (ADS)

Adachi, M.; Kojima, K.; Hayashi, K.

2007-02-01

For the light source of photocoagulators for ophthalmology, orange laser is more suitable than green laser because of low scattering loss by the crystalline lens, and low absorption by xanthophylls in the retina. We developed two orange fiber lasers (580 nm and 590 nm) to investigate the effect depending on the difference in the range of orange. The 580nm laser is composed of a 1160 nm fiber laser and a Periodically Polled Lithium Niobate (PPLN) crystal for second harmonic generation. The 1160 nm fiber laser beam is focused into the MgO-doped PPLN crystal whose length is 30 mm with 3-pass configuration. Continuous-wave 1.3 W output power of 580 nm was obtained with 5.8 W input power of 1160nm for the first time. The conversion efficiency was 22%. The band width of the second harmonic was 0.006 nm (FWHM). The 590 nm laser is almost the same as 580 nm laser source. In this case we used a Raman shift fiber to generate 1180 nm, and the output power of 590 nm was 1.4 W. We developed an evaluation model of photocoagulator system using these two laser sources. A 700 mW coagulation output power was obtained with this orange fiber laser photocoagulator system. This is enough power for the eye surgery. We have the prospect of the maintenance-free, long-life system that is completely air-cooled. We are planning to evaluate this photocoagulator system in order to investigate the difference between the two wavelengths at the field test.

Anaerobic digestion of orange peel in a semi-continuous pilot plant: An environmentally sound way of citrus waste management in agro-ecosystems.

PubMed

Zema, Demetrio A; Fòlino, Adele; Zappia, Giovanni; Calabrò, Paolo S; Tamburino, Vincenzo; Zimbone, Santo Marcello

2018-07-15

The management of residues of citrus processing involves economic and environmental problems. In particular, the uncontrolled disposal of citrus processing waste near production sites can have heavy impacts on air, soil, surface water bodies and groundwater. Anaerobic digestion has been proposed as a viable alternative for citrus waste valorisation, if some problems, linked to the biochemical processes, are overcome. Although many experimental tests have studied the inhibitory effects of the high essential oil content of orange peel on biomethanisation processes, fewer experiences have been carried out in continuous or semi-continuous pilot digesters, more similar to the full-scale biogas plants, using real orange peel. This study has evaluated the methane production through anaerobic digestion of industrial orange peel using a pilot plant (84L) with semi-continuous feeding at increasing Organic Loading Rates (OLR) and essential oil (EO) supply rates (EOsr) until the complete process inhibition. Under mesophilic conditions, the highest daily specific methane yield was achieved at OLR of 1.0g TVS L -1 d -1 and EOsr of 47.6mgL -1 d -1 . Partial inhibition of the anaerobic digestion was detected at OLR and EOsr of 1.98g TVS L -1 d -1 and 88.1mgL -1 d -1 , respectively and the process irreversibly stopped when OLR and EOsr reached 2.5g TVS L -1 d -1 and 111.2mgL -1 d -1 , respectively. Under thermophilic conditions, the cumulative methane production (0.12Lg TVS -1 ) was about 25% of that under mesophilic conditions (0.46Lg TVS -1 ). The thermophilic digestion was completely inhibited at lower OLR (1.98g TVS L -1 d -1 ) and EOsr (88.1mgL -1 d -1 ) compared to mesophilic conditions. This study confirmed the suitability of anaerobic digestion of orange peel for biomethane production (provided that the right management of the process is set), in view of an environmentally sound way of agricultural residues management in agro-ecosystems. Copyright © 2018 Elsevier B.V. All

β-Cryptoxanthin is more bioavailable in humans from fermented orange juice than from orange juice.

PubMed

Hornero-Méndez, Dámaso; Cerrillo, Isabel; Ortega, Ángeles; Rodríguez-Griñolo, María-Rosario; Escudero-López, Blanca; Martín, Franz; Fernández-Pachón, María-Soledad

2018-10-01

Carotenoids, especially β-cryptoxanthin, exert multiple biological activities in the organism. Various processing techniques can improve carotenoid bioavailability in relation to the food matrix. The study objective was to compare the bioavailability of carotenoids from orange juice (OJ) with that from a beverage obtained by alcoholic fermentation of orange juice (FOB). Seven volunteers were recruited for a randomized, controlled, and crossover study. Post-intake plasma carotenoid concentrations were measured by HPLC in the subjects at 0-8 h after their consumption of OJ or FOB. β-Cryptoxanthin and lutein absorption was significantly higher from FOB than from OJ, but no significant difference in zeaxanthin absorption was found. The mean baseline-corrected area under the concentration curve (AUC 0-8 h ) for β-cryptoxanthin, lutein and zeaxanthin was 24.6-, 1.3- and 4.65-fold larger, respectively, after FOB versus OJ consumption. This fermented orange beverage could be an abundant source of bioavailable carotenoids, and its regular consumption may exert healthy effects. Copyright © 2018 Elsevier Ltd. All rights reserved.

Oranges or "lemons"? Family farming and product quality in the Spanish orange industry, 1870-1960.

PubMed

Garrido, Samuel

2010-01-01

In the early twentieth century California became a big exporter of some agricultural products that, until then, had only been grown on a large scale in the mediterranean basin. As a result, exports of those products diminished or stagnated in Mediterranean countries, with important repercussions on their economies. The Spanish orange industry, however, continued to expand, despite the fact that a substantial percentage of Spanish oranges came from farms owned by (often illiterate) small peasants who, in comparison to the California growers, used a great deal of labor, small amounts of capital, and little science. This paper shows that Spanish farmers were in fact capable of growing high-quality oranges at prices that were more competitive than those in California, although interested they often preferred to satisfy the strong demand for middling fruit from Great Britain because it was a more profitable business. This, combined with a deficient use of brand names, gave the Spanish citrus industry serious reputation problems by the 1930s, from which, however, it recovered quickly.

Trouble Brewing in Orange County. Policy Brief

ERIC Educational Resources Information Center

Buck, Stuart

2010-01-01

Orange County will soon face enormous budgetary pressures from the growing deficits in public pensions, both at a state and local level. In this policy brief, the author estimates that Orange County faces a total $41.2 billion liability for retiree benefits that are underfunded--including $9.4 billion for the county pension system and an estimated…

Citrus bergamia essential oil: from basic research to clinical application.

PubMed

Navarra, Michele; Mannucci, Carmen; Delbò, Marisa; Calapai, Gioacchino

2015-01-01

Citrus bergamia Risso et Poiteau, also known as "Bergamot," is a plant belonging to the Rutaceae family, defined as a hybrid of bitter orange and lemon. It is an endemic plant of the Calabria region (Italy). Bergamot fruit is primarily used for the extraction of its essential oil (bergamot essential oil: BEO), employed in perfume, cosmetics, food, and confections. The aim of this review was to collect recent data from the literature on C. bergamia essential oil and, through a critical analysis, focus on safety and the beneficial effects on human health. Clinical studies on the therapeutic applications of BEO exclusively focus on the field of aromatherapy, suggesting that its use can be useful for reducing anxiety and stress.

Growth inhibition of pathogenic bacteria and some yeasts by selected essential oils and survival of L. monocytogenes and C. albicans in apple-carrot juice.

PubMed

Irkin, Reyhan; Korukluoglu, Mihriban

2009-04-01

Food safety is a fundamental concern of both consumers and the food industry. The increasing incidence of foodborne diseases increases the demand of using antimicrobials in foods. Spices and plants are rich in essential oils and show inhibition activity against microorganisms, which are composed of many compounds. In this research, effects of garlic, bay, black pepper, origanum, orange, thyme, tea tree, mint, clove, and cumin essential oils on Listeria monocytogenes AUFE 39237, Escherichia coli ATCC 25922, Salmonella enteritidis ATCC 13076, Proteus mirabilis AUFE 43566, Bacillus cereus AUFE 81154, Saccharomyces uvarum UUFE 16732, Kloeckera apiculata UUFE 10628, Candida albicans ATCC 10231, Candida oleophila UUPP 94365, and Metschnikowia fructicola UUPP 23067 and effects of thyme oil at a concentration of 0.5% on L. monocytogenes and C. albicans in apple-carrot juice during +4 degrees C storage (first to fifth day) were investigated. Strong antibacterial and antifungal activities of some essential oils were found. Thyme, origanum, clove, and orange essential oils were the most inhibitory against bacteria and yeasts. Cumin, tea tree, and mint oils inhibited the yeasts actively. It is concluded that some essential oils could be used as potential biopreservatives capable of controlling foodborne pathogens and food spoilage yeasts.

Integrated Curriculum Activities. Integration of Vocational and Academic Learning through Tech Prep.

ERIC Educational Resources Information Center

Valencia Community Coll., Orlando, FL.

The integrated learning activities in this guide were developed by a committee of educators from Osceola District Schools, Orange County Public Schools, and Valencia Community College (Florida) for a tech prep curriculum. Included are 32 communications-related activities, 30 mathematics activities, and 10 science activities. Each activity includes…

In vitro studies on immunotoxic potential of Orange II in splenocytes.

PubMed

Yadav, Ashish; Kumar, Arvind; Dwivedi, Premendra Dhar; Tripathi, Anurag; Das, Mukul

2012-02-05

Orange II, an azo dye, is not permitted in food preparations, but high levels of the dye have been detected in different food commodities. Though there are reports on the toxicity of Orange II but knowledge based on the immunomodulatory properties of Orange II is scanty. The present investigation was undertaken to study the in vitro immunotoxic potential of Orange II in splenocytes. Splenocytes were isolated, cultured and subjected to immunophenotypic analysis, mixed lymphocyte reaction (MLR) assay or stimulated with lipopolysaccharide (LPS) or concanavalin A (Con A) for 72 h. The supernatant was collected for cytokine assays. Orange II showed cytotoxic effects at 100-1000μg/ml concentrations and 50μg/ml was determined as the highest non-cytotoxic dose. Orange II at the non-cytotoxic dose (50μg/ml) significantly altered the relative distribution of T and B-cells, MLR response and the mitogen induced proliferative response of T-cells and B-cells. Consistent with the hypo-responsiveness of the T and B-lymphocytes, Orange II induced a concomitant decline in the secretion of cytokines IL-2, IL-4, IL-6, IFN-γ, TNF-α and IL-17. On the contrary, there was an increase in the production of IL-10, an anti-inflammatory regulatory cytokine, which may be one of the causative factor for immunosuppressive property of Orange II. These results suggest that non-cytotoxic dose of Orange II may have immunomodulatory effects. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

Genetically engineered orange petunias on the market.

PubMed

Bashandy, Hany; Teeri, Teemu H

2017-08-01

Unauthorized genetically engineered orange petunias were found on the market. Genetic engineering of petunia was shown to lead to novel flower color some 20 years ago. Here we show that petunia lines with orange flowers, generated for scientific purposes, apparently found their way to petunia breeding programmes, intentionally or unintentionally. Today they are widely available, but have not been registered for commerce.

[Standardization of the sour orange flower and leaf].

PubMed

Carnat, A; Carnat, A P; Fraisse, D; Lamaison, J L

1999-09-01

Dried flowers (1 batch) and leaves (6 batches) of sour orange Citrus aurantium L. had a similar flavonoid pattern. But the flavonoid levels of flowers were higher than those of leaves. The mean levels of the principal flavonoid compounds were respectively: total flavonoids 12.35 and 1.06%, neohesperidin 5.44 and 0.08%, naringin 1.93 and 0.06%, eriocitrin 0.38 and 0.25%. 18 batches of commercial origine were also examined for a comparative study. Specifications were proposed for a revision of the monographs "Sour orange flower" and "Sour orange leaf" of the French Pharmacopoeia.

Bioactive compounds from orange epicarp to enrich fish burgers.

PubMed

Spinelli, Sara; Lecce, Lucia; Likyova, Desislava; Del Nobile, Matteo Alessandro; Conte, Amalia

2018-05-01

The orange industry produces considerable amounts of by-products, traditionally used for animal feed or fuel production. Most of these by-products could be used as functional ingredients. To assess the potential food application of orange epicarp, different percentages of micro-encapsulated orange extract were added to fresh fish burgers. Then, an in vitro digestion was also carried out, before and after micro-encapsulation, to measure the bio-accessibility of the active compounds. A significant increase of bio-accessibility of bioactive compounds has been observed in the orange epicarp extract after micro-encapsulation by spray-drying. From the sensory point of view, the fish sample enriched with 50 g kg -1 micro-encapsulated extract was the most comparable to the control burger, even if it showed a higher phenolic, flavonoid and carotenoid bio-accessibility. Orange epicarp may be used as a food additive to enhance the health content of food products. The micro-encapsulation is a valid technique to protect the bioactive compounds and increase their bio-accessibility. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

77 FR 22343 - Certain Orange Juice From Brazil

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-13

... INTERNATIONAL TRADE COMMISSION [Investigation No. 731-TA-1089 (Review)] Certain Orange Juice From Brazil Determination On the basis of the record \\1\\ developed in the subject five-year review, the United... Publication 4311 (April 2012), entitled Certain Orange Juice from Brazil: Investigation No. 731-TA-1089...

Antimicrobial Impacts of Essential Oils on Food Borne-Pathogens.

PubMed

Ozogul, Yesim; Kuley, Esmeray; Ucar, Yilmaz; Ozogul, Fatih

2015-01-01

The antimicrobial activity of twelve essential oil (pine oil, eucalyptus, thyme, sage tea, lavender, orange, laurel, lemon, myrtle, lemon, rosemary and juniper) was tested by a disc diffusion method against food borne pathogens (Escherichia coli, Salmonella paratyphi A, Klebsiella pneumoniae, Yersinia enterocolitica, Pseudomonas aeruginosa, Aeromonas hydrophila, Campylobacter jejuni, Enterococcus faecalis, Staphylococcus aureus). The major components in essential oils were monoterpenes hydrocarbons, α-pinene, limonene; monoterpene phenol, carvacrol and oxygenated monoterpenes, camphor, 1,8-cineole, eucalyptol, linalool and linalyl acetate. Although the antimicrobial effect of essential oils varied depending on the chemical composition of the essential oils and specific microorganism tested, majority of the oils exhibited antibacterial activity against one or more strains. The essential oil with the lowest inhibition zones was juniper with the values varied from 1.5 to 6 mm. However, the components of essential oil of thyme and pine oil are highly active against food borne pathogen, generating the largest inhibition zones for both gram negative and positive bacteria (5.25-28.25 mm vs. 12.5-30 mm inhibition zones). These results indicate the possible use of the essential oils on food system as antimicrobial agents against food-borne pathogen. The article also offers some promising patents on applications of essential oils on food industry as antimicrobial agent.

SU-F-T-63: Dosimetric Relevance of the Valencia and Leipzig HDR Applicators Plastic Cap

DOE Office of Scientific and Technical Information (OSTI.GOV)

Granero, D; Candela-Juan, C; Vijande, J

Purpose: Utilization of HDR brachytherapy treatment of skin lesions using collimated applicators, such as the Valencia or Leipzig is increasing. These applicators are made of cup-shaped tungsten material in order to focalize the radiation into the lesion and to protect nearby tissues. These applicators have an attachable plastic cap that removes secondary electrons generated in the applicator and flattens the treatment surface. The purpose of this study is to examine the dosimetric impact of this cap, and the effect if the cap is not placed during the HDR fraction delivery. Methods: Monte Carlo simulations have been done using the codemore » Geant4 for the Valencia and Leipzig applicators. Dose rate distributions have been obtained for the applicators with and without the plastic cap. An experimental study using EBT3 radiochromic film has been realized in order to verify the Monte Carlo results. Results: The Monte Carlo simulations show that absorbed dose in the first millimeter of skin can increase up to 180% for the Valencia applicator if the plastic cap is absent and up to 1500% for the Leipzig applicators. At deeper distances the increase of dose is smaller being about 10–15%. Conclusion: Important differences have been found if the plastic cap of the applicators is absent in the treatment producing an overdosage in the skin. The user should have a checklist to remind him check always before HDR fraction delivery to insure the plastic cap is placed on the applicator. This work was supported in part by Generalitat Valenciana under Project PROMETEOII/2013/010, by the Spanish Government under Project No. FIS2013-42156, and by a research agreement with Elekta Brachytherapy, Veenendaal, The Netherlands.« less

Red-fleshed sweet orange juice improves the risk factors for metabolic syndrome.

PubMed

Silveira, Jacqueline Q; Dourado, Grace K Z S; Cesar, Thais B

2015-01-01

Orange juice consumption can promote lower levels of oxidative stress and inflammation due to the antioxidant activity of citrus flavonoids and carotenoids. In addition, red-fleshed sweet orange juice (red orange juice) also contains lycopene. This study investigated the effects of red orange juice consumption on risk factors for metabolic syndrome. Volunteers consumed red orange juice daily for 8 weeks, with clinical and biochemical assessments performed at baseline and on the final day. There was no change in the abdominal obesity, but low-density lipoprotein cholesterol, C-reactive protein decreased, while there was an increase of the antioxidant activity in serum after red orange juice consumption. Insulin resistance and systolic blood pressure were reduced in normal-weight volunteers, while diastolic blood pressure decreased in overweight volunteers after intervention. Red orange juice showed anti-inflammatory, antioxidant, and lipid-lowering properties that may prevent the development of metabolic syndrome.

Effects of orange juice on the pharmacokinetics of atenolol.

PubMed

Lilja, J J; Raaska, K; Neuvonen, P J

2005-07-01

Fruit juices can significantly change the pharmacokinetics of several drugs. Our objective was to investigate the effect of orange juice on the pharmacokinetics of the beta-blocking agent atenolol. In a randomized cross-over study with two phases and a washout of 2 weeks, ten healthy volunteers took either 200 ml orange juice or water thrice daily for 3 days and twice on the fourth day. On the morning of day 3, each subject ingested 50 mg atenolol with an additional amount of either 200 ml orange juice or water. The plasma concentrations of atenolol and the cumulative excretion of atenolol into urine were measured up to 33 h after its dosing. Systolic and diastolic blood pressures and heart rate were recorded in a sitting position before the intake of atenolol and 2, 4, 6, and 10 h after. Orange juice decreased the mean peak plasma concentration (C(max)) of atenolol by 49% (range 16-59%, P<0.01), and the mean area under the plasma atenolol concentration-time curve (AUC(0-33 h)) by 40% (range 25-55%, P<0.01). The time of the peak concentration (t(max)) and the elimination half-life (t(1/2)) of atenolol remained unchanged by orange juice. The amount of atenolol excreted into urine was decreased by 38% (range 17-60%, P<0.01), but the renal clearance remained unaltered. The average heart rate was slightly higher during the orange juice+atenolol phase than during the water+atenolol phase. Orange juice moderately interferes with the gastrointestinal absorption of atenolol. This food-drug interaction can be of clinical significance.

21 CFR 146.146 - Frozen concentrated orange juice.

Code of Federal Regulations, 2012 CFR

2012-04-01

... Fruit Juices and Beverages § 146.146 Frozen concentrated orange juice. (a) Frozen concentrated orange... sweetening ingredients specified in paragraph (b) of this section may be added to adjust the final... any added optional sweetening ingredients. The dilution ratio shall be not less than 3 plus 1. For the...

21 CFR 146.146 - Frozen concentrated orange juice.

Code of Federal Regulations, 2014 CFR

2014-04-01

... Fruit Juices and Beverages § 146.146 Frozen concentrated orange juice. (a) Frozen concentrated orange... sweetening ingredients specified in paragraph (b) of this section may be added to adjust the final... any added optional sweetening ingredients. The dilution ratio shall be not less than 3 plus 1. For the...

21 CFR 146.146 - Frozen concentrated orange juice.

Code of Federal Regulations, 2011 CFR

2011-04-01

... Fruit Juices and Beverages § 146.146 Frozen concentrated orange juice. (a) Frozen concentrated orange... sweetening ingredients specified in paragraph (b) of this section may be added to adjust the final... any added optional sweetening ingredients. The dilution ratio shall be not less than 3 plus 1. For the...

21 CFR 146.146 - Frozen concentrated orange juice.

Code of Federal Regulations, 2013 CFR

2013-04-01

... Fruit Juices and Beverages § 146.146 Frozen concentrated orange juice. (a) Frozen concentrated orange... sweetening ingredients specified in paragraph (b) of this section may be added to adjust the final... any added optional sweetening ingredients. The dilution ratio shall be not less than 3 plus 1. For the...

21 CFR 146.146 - Frozen concentrated orange juice.

Code of Federal Regulations, 2010 CFR

2010-04-01

... Fruit Juices and Beverages § 146.146 Frozen concentrated orange juice. (a) Frozen concentrated orange... sweetening ingredients specified in paragraph (b) of this section may be added to adjust the final... any added optional sweetening ingredients. The dilution ratio shall be not less than 3 plus 1. For the...

Development of orange rust of sugarcane in Louisiana

USDA-ARS?s Scientific Manuscript database

Orange rust of sugarcane was observed for the first time in the Americas in 2007 in Florida. Urediniospores of Puccinia kuehnii, the causal agent of orange rust, were collected in aerial traps located in Louisiana in 2010; however, the first observation of disease symptoms in the state was not made ...

Blood orange juice inhibits fat accumulation in mice.

PubMed

Titta, L; Trinei, M; Stendardo, M; Berniakovich, I; Petroni, K; Tonelli, C; Riso, P; Porrini, M; Minucci, S; Pelicci, P G; Rapisarda, P; Reforgiato Recupero, G; Giorgio, M

2010-03-01

To analyze the effect of the juice obtained from two varieties of sweet orange (Citrus sinensis L. Osbeck), Moro (a blood orange) and Navelina (a blond orange), on fat accumulation in mice fed a standard or a high-fat diet (HFD). Obesity was induced in male C57/Bl6 mice by feeding a HFD. Moro and Navelina juices were provided instead of water. The effect of an anthocyanin-enriched extract from Moro oranges or purified cyanidin-3-glucoside (C3G) was also analyzed. Body weight and food intake were measured regularly over a 12-week period. The adipose pads were weighted and analyzed histologically; total RNA was also isolated for microarray analysis. Dietary supplementation of Moro juice, but not Navelina juice significantly reduced body weight gain and fat accumulation regardless of the increased energy intake because of sugar content. Furthermore, mice drinking Moro juice were resistant to HFD-induced obesity with no alterations in food intake. Only the anthocyanin extract, but not the purified C3G, slightly affected fat accumulation. High-throughput gene expression analysis of fat tissues confirmed that Moro juice could entirely rescue the high fat-induced transcriptional reprogramming. Moro juice anti-obesity effect on fat accumulation cannot be explained only by its anthocyanin content. Our findings suggest that multiple components present in the Moro orange juice might act synergistically to inhibit fat accumulation.

Citrus bergamia essential oil: from basic research to clinical application

PubMed Central

Navarra, Michele; Mannucci, Carmen; Delbò, Marisa; Calapai, Gioacchino

2015-01-01

Citrus bergamia Risso et Poiteau, also known as “Bergamot,” is a plant belonging to the Rutaceae family, defined as a hybrid of bitter orange and lemon. It is an endemic plant of the Calabria region (Italy). Bergamot fruit is primarily used for the extraction of its essential oil (bergamot essential oil: BEO), employed in perfume, cosmetics, food, and confections. The aim of this review was to collect recent data from the literature on C. bergamia essential oil and, through a critical analysis, focus on safety and the beneficial effects on human health. Clinical studies on the therapeutic applications of BEO exclusively focus on the field of aromatherapy, suggesting that its use can be useful for reducing anxiety and stress. PMID:25784877

Assessing a traceability technique in fresh oranges (Citrus sinensis L. Osbeck) with an HS-SPME-GC-MS method. Towards a volatile characterisation of organic oranges.

PubMed

Cuevas, Francisco Julián; Moreno-Rojas, José Manuel; Ruiz-Moreno, María José

2017-04-15

A targeted approach using HS-SPME-GC-MS was performed to compare flavour compounds of 'Navelina' and 'Salustiana' orange cultivars from organic and conventional management systems. Both varieties of conventional oranges showed higher content of ester compounds. On the other hand, higher content of some compounds related with the geranyl-diphosphate pathway (neryl and geranyl acetates) and some terpenoids were found in the organic samples. Furthermore, the partial least square discriminant analysis (PLS-DA) achieved an effective classification for oranges based on the farming system using their volatile profiles (90 and 100% correct classification). To our knowledge, it is the first time that a comparative study dealing with farming systems and orange aroma profile has been performed. These new insights, taking into account local databases, cultivars and advanced analytical tools, highlight the potential of volatile composition for organic orange discrimination. Copyright © 2016 Elsevier Ltd. All rights reserved.

Apollo 17 "Orange soil" and meteorite impact on liquid lava

USGS Publications Warehouse

Roedder, E.; Weiblen, P.W.

1973-01-01

THE 'orange soil' from Shorty Crater differs greatly from ordinary lunar soils in that it consists of ???99% 10-300 ??m smooth shiny spherules and broken fragments of spherules of transparent orange glass, about 20% of which contain partly crystallized to opaque material. The remaining 1 % is chiefly crystalline basalt fragments. Although the colour of the individual orange spherule varies with thickness from yellow-orange to red-brown, all orange glass in our sample (74220, 70; 0.25 g) has a uniform index of refraction (??? 1.712). By contrast, other lunar soils contain spherules ranging from 1.50 to 1.75. The orange glass is also completely free of bubbles, to the limit of resolution of the light microscope, whereas bubbles are present in many other spherule samples. The spherules generally appear spherical in a normal microscope mount, but when viewed from two directions many are found to be oblate spheroids with axial ratios varying from near 1.00 to as low as 0.42 (Fig. 1a). Some have fissioned during free flight1 and all stages of the fission process are found, as described for the Apollo 11 samples. Only a few spherules seem to have been distorted by landing while still soft. One notable exception is the occurrence of small spherules of orange glass conforming and adhering to the surface of larger black spherules (Fig. 1b). ?? 1973 Nature Publishing Group.

Identification of sensory attributes that drive consumer liking of commercial orange juice products in Korea.

PubMed

Kim, Mina K; Lee, Young-Jin; Kwak, Han Sub; Kang, Myung-woo

2013-09-01

Orange juice is a well-accepted fruit juice, and its consumption increases steadily. Many studies have been conducted to understand the sensory characteristics of orange juice throughout its varying processing steps. Sensory language and consumer likings of food can be influenced by culture. The objective of this study is to evaluate the sensory characteristics of commercially available orange juices in Korea and identify drivers of liking for orange juices in Korea. A quantitative descriptive analysis was conducted using a trained panel (n = 10) to evaluate 7 orange juice samples in triplicates, followed by consumer acceptance tests (n = 103). Univariate and multivariate statistical analyses were conducted for data analysis. The sensory characteristics of commercially available orange juice were documented and grouped: group 1 samples were characterized by high in natural citrus flavors such as orange peel, orange flesh, citrus fruit, and grape fruit, whereas group 2 samples were characterized by processed orange-like flavors such as over-ripe, cooked-orange, and yogurt. Regardless of orange flavor types, a high intensity of orange flavor in orange juice was identified as a driver of liking for orange juices in Korea. Three distinct clusters were segmented by varying sensory attributes that were evaluated by likes and dislikes. Overall, many similarities were noticed between Korean market segment and global orange juice market. By knowing the drivers of liking and understanding the distinct consumer clusters present in the Korean orange juice market, the orange juice industry could improve the strategic marketing of its products in Korea. © 2013 Institute of Food Technologists®

Growth and yield of valencia, spanish, virginia and runner market type peanuts in various row spacings

USDA-ARS?s Scientific Manuscript database

Currently, the majority of peanuts grown in New Mexico and West Texas are planted in single rows on beds 36 to 40 inches apart. In 2006-2008, several field studies were conducted with Valencia peanuts comparing single row, twin row, and diamond planting patterns in various populations. The basic c...

In Vogue: How Valencia Community College Used a High-Fashion Marketing Campaign to Sharpen Its Image

ERIC Educational Resources Information Center

Campagnuolo, Christian

2008-01-01

Not unlike many community colleges across the country, Valencia Community College, located in Orlando, Florida, has been working to better connect with its constituents. In an era in which the Internet is opening new lines of communication between schools and prospective students, more community colleges are tapping into the opportunities inherent…

Smos Land Product Validation Activities at the Valencia Anchor Station

NASA Astrophysics Data System (ADS)

Lopez-Baeza, Ernesto

ABSTRACT Soil moisture is a key parameter controlling the exchanges between the land surface and the atmosphere. In spite of being important for weather and climate modeling, this parameter is not well observed at a global scale. The SMOS (Soil Moisture and Ocean Salinity) Mission was designed by the European Space Agency (ESA) to measure soil moisture over continental surfaces as well as surface salinity over the oceans. Since 2001, the Valencia Anchor Station is currently being prepared for the validation of SMOS land products, namely soil moisture content and vegetation water content. The site has recently been selected by the Mission as a core validation site, mainly due to the reasonable homogeneous characteristics of the area which make it appropriate to undertake the validation of SMOS Level 2 land products during the Mission Commissioning Phase, before attempting more complex areas. Close to SMOS launch, ESA has defined and designed a SMOS V alidation Rehearsal C ampaign P lan which purpose is to repeat the Commissioning Phase execution with all centers, all tools, all participants, all structures, all data available, assuming all tools and structures are ready and trying to produce as close as possible the post-launch conditions. The aim is to test the readiness, the ensemble coordination and the speed of operations, and to avoid as far as possible any unexpected deficiencies of the plan and procedure during the real C ommissioning P hase campaigns. For the rehearsal activity, a control area of 10 x 10 km2 has been chosen at the Valencia Anchor Station study area where a network of ground soil moisture measuring stations is being set up based on the definition of homogeneous physio-hydrological units, attending to climatic, soil type, lithology, geology, elevation, slope and vegetation cover conditions. These stations are linked via a wireless communication system to a master post accessible via internet. The ground soil moisture stations will also be used

Inhibitors of 15-lipoxygenase from orange peel.

PubMed

Malterud, K E; Rydland, K M

2000-11-01

A series of polymethoxylated flavonoids has been isolated from orange peel, and their inhibitory activity toward soybean 15-lipoxygenase was determined. The strongest inhibition was shown by 3,5,6,7,3',4'-hexamethoxyflavone (IC(50) = 49 +/- 5 microM). Sinensetin, nobiletin, tangeretin, tetramethylscutellarein, and 3,5, 6,7,8,3',4'-heptamethoxyflavone were somewhat less active, with IC(50) values of 70-86 microM, comparable to the positive control quercetin (IC(50) = 68 +/- 5 microM). Demethylation apparently results in less active compounds, with 5-O-demethylsinensetin having an IC(50) value of 144 +/- 10 microM. Some other orange peel constituents were isolated and tested as well, hesperidin (IC(50) = 180 +/- 10 microM) and ferulic acid (111 +/- 2 microM), showing moderate activity. The polymethoxylated flavonoids were virtually inactive as scavengers of the diphenylpicrylhydrazyl radical. Hesperidin was only slightly active (24.2 +/- 0.7% scavenged at a concentration of 2 mM), and ferulic acid showed good activity (IC(50) = 86.4 +/- 0.7 microM). From this, it appears that orange peel constituents may counteract enzymatic lipid peroxidation processes catalyzed by 15-lipoxygenase in vitro. The radical scavenging activity of orange peel extracts is only modest.

ORANGE: a Monte Carlo dose engine for radiotherapy.

PubMed

van der Zee, W; Hogenbirk, A; van der Marck, S C

2005-02-21

This study presents data for the verification of ORANGE, a fast MCNP-based dose engine for radiotherapy treatment planning. In order to verify the new algorithm, it has been benchmarked against DOSXYZ and against measurements. For the benchmarking, first calculations have been done using the ICCR-XIII benchmark. Next, calculations have been done with DOSXYZ and ORANGE in five different phantoms (one homogeneous, two with bone equivalent inserts and two with lung equivalent inserts). The calculations have been done with two mono-energetic photon beams (2 MeV and 6 MeV) and two mono-energetic electron beams (10 MeV and 20 MeV). Comparison of the calculated data (from DOSXYZ and ORANGE) against measurements was possible for a realistic 10 MV photon beam and a realistic 15 MeV electron beam in a homogeneous phantom only. For the comparison of the calculated dose distributions and dose distributions against measurements, the concept of the confidence limit (CL) has been used. This concept reduces the difference between two data sets to a single number, which gives the deviation for 90% of the dose distributions. Using this concept, it was found that ORANGE was always within the statistical bandwidth with DOSXYZ and the measurements. The ICCR-XIII benchmark showed that ORANGE is seven times faster than DOSXYZ, a result comparable with other accelerated Monte Carlo dose systems when no variance reduction is used. As shown for XVMC, using variance reduction techniques has the potential for further acceleration. Using modern computer hardware, this brings the total calculation time for a dose distribution with 1.5% (statistical) accuracy within the clinical range (less then 10 min). This means that ORANGE can be a candidate for a dose engine in radiotherapy treatment planning.

Empirical prediction and validation of antibacterial inhibitory effects of various plant essential oils on common pathogenic bacteria.

PubMed

Akdemir Evrendilek, Gulsun

2015-06-02

In this study, fractional compound composition, antioxidant capacity, and phenolic substance content of 14 plant essential oils-anise (Pimpinella anisum), bay leaves (Laurus nobilis), cinnamon bark (Cinnamomum verum), clove (Eugenia caryophyllata), fennel (Foeniculum vulgare), hop (Humulus lupulus), Istanbul oregano (Origanum vulgare subsp. hirtum), Izmir oregano (Origanum onites), mint (Mentha piperita), myrtus (Myrtus communis), orange peel (Citrus sinensis), sage (Salvia officinalis), thyme (Thymbra spicata), and Turkish oregano (Origanum minutiflorum)--were related to inhibition of 10 bacteria through multiple linear or non-linear (M(N)LR) models-four Gram-positive bacteria of Listeria innocua, coagulase-negative staphylococci, Staphylococcus aureus, and Bacillus subtilis, and six Gram-negative bacteria of Yersinia enterocolitica, Salmonella Enteritidis, Salmonella Typhimurium, Proteus mirabilis, Escherichia coli O157:H7, and Klebsiella oxytoca. A total of 65 compounds with different antioxidant capacity, phenolic substance content and antibacterial properties were detected with 14 plant essential oils. The best-fit M(N)LR models indicated that relative to anise essential oil, the essential oils of oreganos, cinnamon, and thyme had consistently high inhibitory effects, while orange peel essential oil had consistently a low inhibitory effect. Regression analysis indicated that beta-bisabolene (Turkish and Istanbul oreganos), and terpinolene (thyme) were found to be the most inhibitory compounds regardless of the bacteria type tested. Copyright © 2015 Elsevier B.V. All rights reserved.

Soil erosion after forest fires in the Valencia region

NASA Astrophysics Data System (ADS)

González-Pelayo, Óscar; Keizer, Jan Jacob; Cerdà, Artemi

2014-05-01

Soil erosion after forest fire is triggered by the lack of vegetation cover and the degradation of the physical, biological and chemical properties (Martí et al., 2012; Fernández et al., 2012; Guénon, 2013). Valencia region belongs to the west Mediterranean basin ("Csa", Köppen climate classification), with drought summer periods that enhance forest fire risk. The characteristics of the climate, lithology and land use history makes this region more vulnerable to soil erosion. In this area, fire recurrence is being increased since late 50s (Pausas, 2004) and post-fire erosion studies became more popular from 80's until nowadays (Cerdá and Mataix-Solera, 2009). Research in Valencia region has contributed significantly to a better understanding of the effect of spatial and temporal scale on runoff and sediment yield measurements. The main achievements concerns: a) direct measurement of erosion rates under a wide range of methodologies (natural vs simulated rainfall, open vs closed plots); from micro- to meso-plot and catchment scale in single (Rubio et al., 1994; Cerdà et al., 1995; Cerdà 1998a; 1998b; Llovet et al., 1998; Cerdà, 2001; Calvo-Cases et al., 2003; Andreu et al., 2001; Mayor et al., 2007; Cerdà and Doerr, 2008) and multiples fires (Campo et al., 2006; González-Pelayo et al., 2010a). Changes in soil properties (Sanroque et al., 1985; Rubio et al., 1997; Boix-Fayós, 1997; Gimeno-Garcia et al., 2000; Guerrero et al., 2001; Mataix-Solera et al., 2004; González-Pelayo et al., 2006; Arcenegui et al., 2008; Campo et al., 2008; Bodí et al., 2012), in post-fire vegetation patterns (Gimeno-García et al., 2007) and, studies on mitigation strategies (Bautista et al., 1996; Abad et al., 2000). b) Progress to understanding post-fire erosion mechanism and sediment movement (Boix-Fayós et al., 2005) by definition of thresholds for sediment losses; fire severity, slope angle, bedrock, rain characteristics, vegetation pattern and ecosystem resilience (Mayor

Orange-red emitting Gd2Zr2O7:Sm3+: Structure-property correlation, optical properties and defect spectroscopy

NASA Astrophysics Data System (ADS)

Gupta, Santosh K.; Reghukumar, C.; Sudarshan, K.; Ghosh, P. S.; Pathak, Nimai; Kadam, R. M.

2018-05-01

Local structure analysis of dopant ion, understanding host to dopant energy transfer dynamics and defects characterization in a doped material which plays an important role in the designing a highly efficient opto-electronic material. In this connection a new Sm3+ doped Gd2Zr2O7 pyrochlore material was synthesized using gel-combustion technique and was characterized systematically using X-ray diffraction (XRD), time resolved photoluminescence spectroscopy (TRPLS), positron annihilation lifetime spectroscopy (PALS) and density functional theory (DFT) based ab-initio calculation. Based on DFT site selective energetics calculation and luminescence decay measurement, it was observed that the Sm3+ was distributed at both Gd3+ and Zr4+ site with higher Sm3+ fraction at the Gd3+ site. PALS was used to probe the presence of defects in the phosphor. In this work intense orange-red emission is realized through manipulating the energy transfer from host defect emission (oxygen vacancies) to Sm3+ which allows color emission from green in undoped to orange-red in doped samples. Effect of dopant concentration and annealing temperature was probed using TRPLS and PALS. These all information is highly important for researcher looking to achieve pyrochlore based phosphor materials with high quantum yield.

Crystal structure of phototoxic orange fluorescent proteins with α tryptophan-based chromophore

DOE PAGES

Pletneva, Nadya V.; Pletnev, Vladimir Z.; Sarkisyan, Karen S.; ...

2015-12-23

Phototoxic fluorescent proteins represent a sparse group of genetically encoded photosensitizers that could be used for precise light-induced inactivation of target proteins, DNA damage, and cell killing. Only two such GFP-based fluorescent proteins (FPs), KillerRed and its monomeric variant SuperNova, were described up to date. We present a crystallographic study of their two orange successors, dimeric KillerOrange and monomeric mKiller-Orange, at 1.81 and 1.57 Å resolution, respectively. They are the first orange-emitting protein photosensitizers with a tryptophan-based chromophore (Gln65-Trp66-Gly67). Same as their red progenitors, both orange photosensitizers have a water-filled channel connecting the chromophore to the β-barrel exterior and enablingmore » transport of ROS. In both proteins, Trp66 of the chromophore adopts an unusual trans-cis conformation stabilized by H-bond with the nearby Gln159. This trans-cis conformation along with the water channel was shown to be a key structural feature providing bright orange emission and phototoxicity of both examined orange photosensitizers.« less

Crystal Structure of Phototoxic Orange Fluorescent Proteins with a Tryptophan-Based Chromophore

PubMed Central

Pletneva, Nadya V.; Pletnev, Vladimir Z.; Sarkisyan, Karen S.; Gorbachev, Dmitry A.; Egorov, Evgeny S.; Mishin, Alexander S.; Lukyanov, Konstantin A.; Dauter, Zbigniew; Pletnev, Sergei

2015-01-01

Phototoxic fluorescent proteins represent a sparse group of genetically encoded photosensitizers that could be used for precise light-induced inactivation of target proteins, DNA damage, and cell killing. Only two such GFP-based fluorescent proteins (FPs), KillerRed and its monomeric variant SuperNova, were described up to date. Here, we present a crystallographic study of their two orange successors, dimeric KillerOrange and monomeric mKillerOrange, at 1.81 and 1.57 Å resolution, respectively. They are the first orange-emitting protein photosensitizers with a tryptophan-based chromophore (Gln65-Trp66-Gly67). Same as their red progenitors, both orange photosensitizers have a water-filled channel connecting the chromophore to the β-barrel exterior and enabling transport of ROS. In both proteins, Trp66 of the chromophore adopts an unusual trans-cis conformation stabilized by H-bond with the nearby Gln159. This trans-cis conformation along with the water channel was shown to be a key structural feature providing bright orange emission and phototoxicity of both examined orange photosensitizers. PMID:26699366

Literacy and Development for the Orang Asli in Malaysia: What Matters?

ERIC Educational Resources Information Center

Renganathan, Sumathi

2016-01-01

This article explores the literacy practices of the indigenous Semai Orang Asli community in Malaysia. Literacy for the Orang Asli often centres on formal education and schooling and is hardly explored from a social and cultural perspective. In fact, researchers have paid barely any attention to Orang Asli oral and literate traditions nor their…

Ultrastructural changes in sweet orange with symptoms of huanglongbing

USDA-ARS?s Scientific Manuscript database

Citrus greening (Huanglongbing [HLB]) is one of the most destructive citrus diseases worldwide. To better understand the ultrastructural changes of sweet orange seedlings in response to infection, anatomical analyses of HLB-infected sweet orange were carried out by light and electron microscopy. A...

EDTA aggregates induce SYPRO orange-based fluorescence in thermal shift assay

PubMed Central

Kroeger, Tobias; Frieg, Benedikt; Zhang, Tao; Hansen, Finn K.; Marmann, Andreas; Proksch, Peter; Nagel-Steger, Luitgard; Groth, Georg; Smits, Sander H. J.

2017-01-01

Ethylenediaminetetraacetic acid (EDTA) is widely used in the life sciences as chelating ligand of metal ions. However, formation of supramolecular EDTA aggregates at pH > 8 has been reported, which may lead to artifactual assay results. When applied as a buffer component at pH ≈ 10 in differential scanning fluorimetry (TSA) using SYPRO Orange as fluorescent dye, we observed a sharp change in fluorescence intensity about 20°C lower than expected for the investigated protein. We hypothesized that this change results from SYPRO Orange/EDTA interactions. TSA experiments in the presence of SYPRO Orange using solutions that contain EDTA-Na+ but no protein were performed. The TSA experiments provide evidence that suggests that at pH > 9, EDTA4- interacts with SYPRO Orange in a temperature-dependent manner, leading to a fluorescence signal yielding a “denaturation temperature” of ~68°C. Titrating Ca2+ to SYPRO Orange and EDTA solutions quenched fluorescence. Ethylene glycol tetraacetic acid (EGTA) behaved similarly to EDTA. Analytical ultracentrifugation corroborated the formation of EDTA aggregates. Molecular dynamics simulations of free diffusion of EDTA-Na+ and SYPRO Orange of in total 27 μs suggested the first structural model of EDTA aggregates in which U-shaped EDTA4- arrange in an inverse bilayer-like manner, exposing ethylene moieties to the solvent, with which SYPRO Orange interacts. We conclude that EDTA aggregates induce a SYPRO Orange-based fluorescence in TSA. These results make it relevant to ascertain that future TSA results are not influenced by interference between EDTA, or EDTA-related molecules, and the fluorescent dye. PMID:28472107

Influence of lipid type on gastrointestinal fate of oil-in-water emulsions: In vitro digestion study.

PubMed

Zhang, Ruojie; Zhang, Zipei; Zhang, Hui; Decker, Eric Andrew; McClements, David Julian

2015-09-01

The potential gastrointestinal fate of oil-in-water emulsions containing lipid phases from different sources was examined: vegetable oils (corn, olive, sunflower, and canola oil); marine oils (fish and krill oil); flavor oils (orange and lemon oil); and, medium chain triglycerides (MCT). The lowest rates and extents of lipid digestion were observed for emulsified flavor oil, followed by emulsified krill oil. There was no appreciable difference between the final amounts of free fatty acids released for emulsified digestible oils. Differences in the digestibility of emulsions prepared using different oils were attributed to differences in their compositions, e.g., fatty acid chain length and unsaturation. The particle size distribution, particle charge, microstructure, and macroscopic appearance of the emulsions during passage through the simulated GIT depended on oil type. The results of this study may facilitate the design of functional foods that control the digestion and absorption of triglycerides, as well as the bioaccessibility of hydrophobic bioactives. Copyright © 2015 Elsevier Ltd. All rights reserved.

Pharmacokinetics of flavanone glycosides after ingestion of single doses of fresh-squeezed orange juice versus commercially processed orange juice in healthy humans

USDA-ARS?s Scientific Manuscript database

Orange juice is a rich source of flavonoids known to be beneficial to cardiovascular health in humans. The objective of this study was to analyze the pharmacokinetics of the main flavanone glycosides, hesperidin and narirutin, in humans after the consumption of two types of orange juice, fresh squee...

The Minorca Basin: a buffer zone between the Valencia and Liguro-Provençal Basins (NW Mediterranean Sea)

NASA Astrophysics Data System (ADS)

Pellen, Romain; Aslanian, Daniel; Rabineau, Marina; Leroux, Estelle; Gorini, Christian; Silenziario, Carmine; Blanpied, Christian; Rubino, Jean-Loup

2017-04-01

The present-day compartmented Mediterranean physiography is inherited from the last 250 Ma kinematic plate evolution (Eurasian, Africa, Iberic and Nubia plates) which implied the formation of orogenic chains, polyphased basins, and morphological - geodynamic thresholds. The interactions between these entities are strongly debated in the North-Western Mediterranean area. Several Neogene reconstructions have been proposed for the Valencia basin depending of the basin segmentation where each model imply a different subsidence, sedimentary, and palaeo-environmental evolution. Our study propose a new kinematic model for the Valencia Basin (VB) that encompasses the sedimentary infill, vertical movement and basin segmentation. Detailed analyses of seismic profiles and boreholes in the VB reveal a differentiated basin, the Minorca Basin (MB), lying between the old Mesozoic Valencia Basin sensu strico (VBss) and the young Oligocene Liguro-Provencal Basin (LPB) (Pellen et al., 2016). The relationship between these basins is shown through the correlation of four Miocene-to-present-day megasequences. The Central and North Balearic Fracture Zones (CFZ and NBFZ) that border the MB represent two morphological and geodynamical thresholds that created an accommodation in steps between the three domains. Little to no horizontal Neogene movements have been found for the Ibiza and Majorca Islands and imply a vertical "sag" subsidence. In contrast, the counterclockwise movement of the Corso-Sardinian blocks induced a counterclockwise movement of the Minorca block towards the SE along the CFZ and NBFZ, during the exhumation of lower continental crust in the LPB. The South-Eastward Minorca block translation stops when the first atypical oceanic crust occurs. The influence of the Neogene Betic compressional phase is thus limited to the VBss on the basis of a different MB origin. This new understanding places the AlKaPeCa blocks northeastward of the present-day Alboran Area. Both NW-SE and

Meat-eating by a wild Bornean orang-utan (Pongo pygmaeus).

PubMed

Buckley, Benjamin J W; Dench, Rosalie J; Morrogh-Bernard, Helen C; Bustani, Unyil; Chivers, David J

2015-10-01

We present the first evidence for consumption of meat by a wild Bornean orang-utan (Pongo pygmaeus). Meat-eating has been reported in Sumatran orang-utans, specifically the hunting and consumption of slow lorises (Nycticebus coucang), but loris-hunting behaviour has not been observed in the Bornean species and meat of any species is essentially absent from their diet, with only two anecdotal reports of vertebrate meat consumption prior to this current finding in over 40 years of study. In August 2012 an unhabituated adult flanged male orang-utan was observed eating an adult horse-tailed squirrel (Sundasciurus hippurus) carcass in the Sabangau peat-swamp forest, Central Kalimantan. We suspect this to be a case of scavenging, never reported previously in a Bornean orang-utan.

UV-blocking potential of oils and juices.

PubMed

Gause, S; Chauhan, A

2016-08-01

Sunscreens are commonly used to protect the body from damage caused by UV light. Some components of organic sunscreens have been shown to pass through the skin during wear which could raise toxicity concerns for these compounds. This study explores the potential for oils and fruit and vegetable juices to be substitutes for these compounds. The absorptivity of various oils (canola oil, citronella oil, coconut oil, olive oil, soya bean oil, vitamin E, as well as aloe vera) and fruit and vegetable juices (acerola, beet, grape, orange carrot, purple carrot and raspberry) was measured in vitro. The mean absorptivity was compared with FDA-approved UV absorbers to gauge the potential of the natural products. The most promising candidates were incorporated into formulations, and the UV transmittance of a 20-μm-thick film of the formulation was measured. The formulations were also imaged by light microscopy and scanning electron microscopy. The absorptivity of oils was at least two orders of magnitude lower compared to the commercial UV blockers. The fruit juice powders were more effective at UV blocking but still showed an order of magnitude lower absorptivity compared to commercial UV blockers. The UV blocking from most natural oils is insufficient to obtain a significant UV protection. Formulations containing 50wt% purple carrot showed good UV-blocking capabilities and represent a promising ingredient for sunscreen and cosmetic applications. © 2015 Society of Cosmetic Scientists and the Société Française de Cosmétologie.

The effect of drying temperatures on antioxidant activity, phenolic compounds, fatty acid composition and tocopherol contents in citrus seed and oils.

PubMed

Al Juhaimi, Fahad; Özcan, Mehmet Musa; Uslu, Nurhan; Ghafoor, Kashif

2018-01-01

In this study, the effect of drying temperature on antioxidant activity, phenolic compounds, fatty acid composition and tocopherol content of citrus seeds and oils were studied. Kinnow mandarin seed, dried at 60 °C, exhibited the highest antioxidant activity. Orlendo orange seed had the maximum total phenolic content and α-tocopherol content, with a value of 63.349 mg/100 g and 28.085 mg/g (control samples), respectively. The antioxidant activity of Orlendo orange seed (63.349%) was higher than seeds of Eureka lemon (55.819%) and Kinnow mandarin (28.015%), while the highest total phenolic content was found in seeds of Kinnow mandarin, followed by Orlendo orange and Eureka lemon (113.132). 1.2-Dihydroxybenzene (13.171), kaempferol (10.780), (+)-catechin (9.341) and isorhamnetin (7.592) in mg/100 g were the major phenolic compounds found in Kinnow mandarin. Among the unsaturated fatty acids, linoleic acid was the most abundant acid in all oils, which varied from 44.4% (dried at 80 °C) to 46.1% (dried at 70 °C), from 39.0% (dried at 60 °C) to 40.0% (dried at 70 °C). The total phenolic content, antioxidant activity and phenolic compounds of citrus seeds and tocopherol content of seed oils were significantly affected by drying process and varied depending on the drying temperature.

Yield, Esterification Degree and Molecular Weight Evaluation of Pectins Isolated from Orange and Grapefruit Peels under Different Conditions

PubMed Central

Sayah, Mohamed Yassine; Chabir, Rachida; Benyahia, Hamid; Rodi Kandri, Youssef; Ouazzani Chahdi, Fouad; Touzani, Hanan; Errachidi, Faouzi

2016-01-01

Orange (Citrus sinensis) and grapefruit (Citrus paradise) peels were used as a source of pectin, which was extracted under different conditions. The peels are used under two states: fresh and residual (after essential oil extraction). Organic acid (citric acid) and mineral acid (sulfuric acid) were used in the pectin extraction. The aim of this study is the evaluation the effect of extraction conditions on pectin yield, degree of esterification “DE” and on molecular weight “Mw”. Results showed that the pectin yield was higher using the residual peels. Moreover, both peels allow the obtainment of a high methoxyl pectin with DE >50%. The molecular weight was calculated using Mark-Houwink-Sakurada equation which describes its relationship with intrinsic viscosity. This later was determined using four equations; Huggins equation, kramer, Schulz-Blaschke and Martin equation. The molecular weight varied from 1.538 x1005 to 2.47x1005 g/mol for grapefruit pectin and from 1.639 x1005 to 2.471 x1005 g/mol for orange pectin. PMID:27644093

A FRET-facilitated photoswitching using an orange fluorescent protein with the fast photoconversion kinetics.

PubMed

Subach, Oksana M; Entenberg, David; Condeelis, John S; Verkhusha, Vladislav V

2012-09-12

Fluorescent proteins photoswitchable with noncytotoxic light irradiation and spectrally distinct from multiple available photoconvertible green-to-red probes are in high demand. We have developed a monomeric fluorescent protein, called PSmOrange2, which is photoswitchable with blue light from an orange (ex./em. at 546 nm/561 nm) to a far-red (ex./em. at 619 nm/651 nm) form. Compared to another orange-to-far-red photoconvertable variant, PSmOrange2 has blue-shifted photoswitching action spectrum, 9-fold higher photoconversion contrast, and up to 10-fold faster photoswitching kinetics. This results in the 4-fold more PSmOrange2 molecules being photoconverted in mammalian cells. Compared to common orange fluorescent proteins, such as mOrange, the orange form of PSmOrange has substantially higher photostability allowing its use in multicolor imaging applications to track dynamics of multiple populations of intracellular objects. The PSmOrange2 photochemical properties allow its efficient photoswitching with common two-photon lasers and, moreover, via Förster resonance energy transfer (FRET) from green fluorescent donors. We have termed the latter effect a FRET-facilitated photoswitching and demonstrated it using several sets of interacting proteins. The enhanced photoswitching properties of PSmOrange2 make it a superior photoconvertable protein tag for flow cytometry, conventional microscopy, and two-photon imaging of live cells.

Characterization of the PTW SourceCheck ionization chamber with the Valencia lodgment for (125)I seed verification.

PubMed

Tornero-López, Ana M; Torres Del Río, Julia; Ruiz, Carmen; Perez-Calatayud, Jose; Guirado, Damián; Lallena, Antonio M

2015-12-01

In brachytherapy using (125)I seed implants, a verification of the air kerma strength of the sources used is required. Typically, between 40 and 100 seeds are implanted. Checking all of them is unaffordable, especially when seeds are disposed in sterile cartridges. Recently, a new procedure allowing the accomplishment of the international recommendations has been proposed for the seedSelectron system of Elekta Brachytherapy. In this procedure, the SourceCheck ionization chamber is used with a special lodgment (Valencia lodgment) that allows to measure up to 10 seeds simultaneously. In this work we analyze this procedure, showing the feasibility of the approximations required for its application, as well as the effect of the additional dependence with the air density that shows the chamber model used. Uncertainty calculations and the verification of the approximation needed to obtain a calibration factor for the Valencia lodgment are carried out. The results of the present work show that the chamber dependence with the air density is the same whether the Valencia lodgment is used or not. On the contrary, the chamber response profile is influenced by the presence of the lodgment. The determination of this profile requires various measurements due to the nonnegligible variability found between different experiments. If it is considered, the uncertainty in the determination of the air-kerma strength increases from 0.5% to 1%. Otherwise, a systematic additional uncertainty of 1% would occur. This could be relevant for the comparison between user and manufacturer measurements that is mandatory in the case studied here. Copyright © 2015 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.

21 CFR 74.2260 - D&C Orange No. 10.

Code of Federal Regulations, 2010 CFR

2010-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2260 D&C Orange No. 10. (a) Identity and specifications. The color additive D&C Orange No. 10 shall conform in identity and specifications to the... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...

21 CFR 74.2261 - D&C Orange No. 11.

Code of Federal Regulations, 2010 CFR

2010-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2261 D&C Orange No. 11. (a) Identity and specifications. The color additive D&C Orange No. 11 shall conform in identity and specifications to the... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...

21 CFR 74.2254 - D&C Orange No. 4.

Code of Federal Regulations, 2010 CFR

2010-04-01

... ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2254 D&C Orange No. 4. (a) Identity and specifications. The color additive D&C Orange No. 4 shall conform in identity and specifications to the requirements... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...

21 CFR 74.2254 - D&C Orange No. 4.

Code of Federal Regulations, 2012 CFR

2012-04-01

... ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2254 D&C Orange No. 4. (a) Identity and specifications. The color additive D&C Orange No. 4 shall conform in identity and specifications to the requirements... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...

21 CFR 74.2260 - D&C Orange No. 10.

Code of Federal Regulations, 2013 CFR

2013-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2260 D&C Orange No. 10. (a) Identity and specifications. The color additive D&C Orange No. 10 shall conform in identity and specifications to the... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...

21 CFR 74.2260 - D&C Orange No. 10.

Code of Federal Regulations, 2012 CFR

2012-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2260 D&C Orange No. 10. (a) Identity and specifications. The color additive D&C Orange No. 10 shall conform in identity and specifications to the... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...

21 CFR 74.2261 - D&C Orange No. 11.

Code of Federal Regulations, 2014 CFR

2014-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2261 D&C Orange No. 11. (a) Identity and specifications. The color additive D&C Orange No. 11 shall conform in identity and specifications to the... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...

21 CFR 74.2261 - D&C Orange No. 11.

Code of Federal Regulations, 2013 CFR

2013-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2261 D&C Orange No. 11. (a) Identity and specifications. The color additive D&C Orange No. 11 shall conform in identity and specifications to the... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...

21 CFR 74.2254 - D&C Orange No. 4.

Code of Federal Regulations, 2013 CFR

2013-04-01

... ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2254 D&C Orange No. 4. (a) Identity and specifications. The color additive D&C Orange No. 4 shall conform in identity and specifications to the requirements... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...

21 CFR 74.2260 - D&C Orange No. 10.

Code of Federal Regulations, 2014 CFR

2014-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2260 D&C Orange No. 10. (a) Identity and specifications. The color additive D&C Orange No. 10 shall conform in identity and specifications to the... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...

21 CFR 74.2261 - D&C Orange No. 11.

Code of Federal Regulations, 2012 CFR

2012-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2261 D&C Orange No. 11. (a) Identity and specifications. The color additive D&C Orange No. 11 shall conform in identity and specifications to the... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...

21 CFR 74.2254 - D&C Orange No. 4.

Code of Federal Regulations, 2014 CFR

2014-04-01

... ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2254 D&C Orange No. 4. (a) Identity and specifications. The color additive D&C Orange No. 4 shall conform in identity and specifications to the requirements... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...

Distribution of retinal cone photoreceptor oil droplets, and identification of associated carotenoids in crow (Corvus macrorhynchos).

PubMed

Rahman, Mohammad Lutfur; Yoshida, Kazuyuki; Maeda, Isamu; Tanaka, Hideuki; Sugita, Shoei

2010-06-01

The topography of cone oil droplets and their carotenoids were investigated in the retina of jungle crow (Corvus macrorhynchos). Fresh retina was sampled for the study of retinal cone oil droplets, and extracted retinal carotenoids were saponified using methods adapted from a recent study, then identified with reverse-phase high-performance liquid chromatography (HPLC). To assess the effects of saponification conditions on carotenoid recovery from crow retina, we varied base concentration and total time of saponification across a wide range of conditions, and again used HPLC to compare carotenoid concentrations. Based on colors, at least four types of oil droplets were recognized, i.e., red, orange, green, and translucent, across the retina. With an average of 91,202 /mm(2), density gradually declines in an eccentric manner from optic disc. In retina, the density and size of droplets are inversely related. In the peripheral zone, oil droplets were significantly larger than those of the central area. The proportion of orange oil droplets (33%) was higher in the central area, whereas green was predominant in other areas. Three types of carotenoid (astaxanthin, galloxanthin and lutein), together with one unknown carotenoid, were recovered from the crow retina; astaxanthin was the dominant carotenoid among them. The recovery of carotenoids was affected by saponification conditions. Astaxanthin was well recovered in weak alkali (0.06 M KOH), in contrast, xanthophyllic carotenoids were best recovered in strong alkali (0.6 M KOH) after 12 h of saponification at freeze temperature.

Decoding the Nonvolatile Sensometabolome of Orange Juice ( Citrus sinensis).

PubMed

Glabasnia, Anneke; Dunkel, Andreas; Frank, Oliver; Hofmann, Thomas

2018-03-14

Activity-guided fractionation in combination with the taste dilution analysis, followed by liquid chromatography-tandem mass spectrometry and nuclear magnetic resonance experiments, led to the identification of 10 polymethoxylated flavones (PMFs), 6 limonoid glucosides, and 2 limonoid aglycones as the key bitterns of orange juice. Quantitative studies and calculation of dose-over-threshold factors, followed by taste re-engineering, demonstrated for the first time 25 sensometabolites to be sufficient to reconstruct the typical taste profile of orange juices and indicated that not a single compound can be considered a suitable marker for juice bitterness. Intriguingly, the taste percept of orange juice seems to be created by a rather complex interplay of limonin, limonoid glucosides, PMFs, organic acids, and sugars. For the first time, sub-threshold concentrations of PMFs were shown to enhance the perceived bitterness of limonoids. Moreover, the influence of sugars on the perceived bitterness of limonoids and PMFs in orange juice relevant concentration ranges was quantitatively elucidated.

[Changes in polyamine levels in Citrus sinensis Osb. cv. Valencia callus during somatic embryogenesis].

PubMed

Liu, Hua-Ying; Xiao, Lang-Tao; Lu, Xu-Dong; Hu, Jia-Jin; Wu, Shun; He, Chang-Zheng; Deng, Xiu-Xin

2005-06-01

Somatic embryogenetic capability and changes in polyamine level and their relationship were analyzed using the long-term (8 years) subcultured calli of Citrus sinensis Osb. cv. Valencia as materials. The results showed that endogenous polyamine contents in embryogenic calli were higher than those in non-embryogenic calli, and the embryogenetic capability was positively correlated to the levels of endogenous polyamines. When the calli were transferred to a differentiation medium, the putrescine content rapidly increased and reached a peak, then fell gradually. Applying exogenous putrescine raised the embryogenesis frequency and endogenous putrescine level. It indicated that increase in putrescine content at early stage of differentiation promoted embryogenesis. With the development of somatic embryo, spermidine content reached its the highest level at globular embryo stage, spermine content rose and reached a peak at a later stage of globular embryo development. Furthermore, changes of the putrescine, spermidine and spermine contents during somatic embryogenesis were similar in Valencia calli which had different ploidy levels, but their contents decreased following the increasing of ploidy level. Changes in arginine decarboxylase activity were positively correlated to the polyamine levels, which suggest that the later is a key factor in regulating the polyamine levels during somatic embryogenesis in citrus plants.

Travel and Tourism Students' Needs in Valencia (Spain): Meeting their Professional Requirements in the ESP Classroom

ERIC Educational Resources Information Center

Garcia Laborda, Jesus

2003-01-01

The main purpose of this paper is to describe the basic findings obtained as a result of the implementation of two projects of Computer and Information Technologies held in Valencia (Spain) between 2002 and 2003 with 92 second year university students enrolled in English as a foreign language to find out their ICT and foreign language needs both…

Effect of ultrahigh-temperature continuous ohmic heating treatment on fresh orange juice.

PubMed

Leizerson, Shirly; Shimoni, Eyal

2005-05-04

The scope of this study is the effect of ohmic heating thermal treatment on liquid fruit juice made of oranges. Effects of ohmic heating on the quality of orange juice were examined and compared to those of heat pasteurization at 90 degrees C for 50 s. Orange juice was treated at temperatures of 90, 120, and 150 degrees C for 1.13, 0.85, and 0.68 s in an ohmic heating system. Microbial counts showed complete inactivation of bacteria, yeast, and mold during ohmic and conventional treatments. The ohmic heating treatment reduced pectin esterase activity by 98%. The reduction in vitamin C was 15%. Ohmic-heated orange juice maintained higher amounts of the five representative flavor compounds than did heat-pasteurized juice. Sensory evaluation tests showed no difference between fresh and ohmic-heated orange juice. Thus, high-temperature ohmic-heating treatment can be effectively used to pasteurize fresh orange juice with minimal sensory deterioration.

Orange juice (poly)phenols are highly bioavailable in humans.

PubMed

Pereira-Caro, Gema; Borges, Gina; van der Hooft, Justin; Clifford, Michael N; Del Rio, Daniele; Lean, Michael E J; Roberts, Susan A; Kellerhals, Michele B; Crozier, Alan

2014-11-01

We assessed the bioavailability of orange juice (poly)phenols by monitoring urinary flavanone metabolites and ring fission catabolites produced by the action of the colonic microbiota. Our objective was to identify and quantify metabolites and catabolites excreted in urine 0-24 h after the acute ingestion of a (poly)phenol-rich orange juice by 12 volunteers. Twelve volunteers [6 men and 6 women; body mass index (in kg/m(2)): 23.9-37.2] consumed a low (poly)phenol diet for 2 d before first drinking 250 mL pulp-enriched orange juice, which contained 584 μmol (poly)phenols of which 537 μmol were flavanones, and after a 2-wk washout, the procedure was repeated, and a placebo drink was consumed. Urine collected for a 24-h period was analyzed qualitatively and quantitatively by using high-performance liquid chromatography-mass spectrometry (HPLC-MS) and gas chromatography-mass spectrometry (GC-MS). A total of 14 metabolites were identified and quantified in urine by using HPLC-MS after orange juice intake. Hesperetin-O-glucuronides, naringenin-O-glucuronides, and hesperetin-3'-O-sulfate were the main metabolites. The overall urinary excretion of flavanone metabolites corresponded to 16% of the intake of 584 μmol (poly)phenols. The GC-MS analysis revealed that 8 urinary catabolites were also excreted in significantly higher quantities after orange juice consumption. These catabolites were 3-(3'-methoxy-4'-hydroxyphenyl)propionic acid, 3-(3'-hydroxy-4'-methoxyphenyl)propionic acid, 3-(3'-hydroxy-4'-methoxyphenyl)hydracrylic acid, 3-(3'-hydroxyphenyl)hydracrylic acid, 3'-methoxy-4'-hydroxyphenylacetic acid, hippuric acid, 3'-hydroxyhippuric acid, and 4'-hydroxyhippuric acid. These aromatic acids originated from the colonic microbiota-mediated breakdown of orange juice (poly)phenols and were excreted in amounts equivalent to 88% of (poly)phenol intake. When combined with the 16% excretion of metabolites, this percentage raised the overall urinary excretion to ∼ 100% of

Orange County intelligent vehicle/highway systems study : IVHS action plan

DOT National Transportation Integrated Search

1997-01-21

This report is a result of the efforts undertaken for Task 6 of the Orange County : Intelligent Vehicle-Highway Systems (IVHS) Study, the development of an Action Plan for : implementation of the Orange County IVHS Master Plan.

Maillard-Reaction-Functionalized Egg Ovalbumin Stabilizes Oil Nanoemulsions.

PubMed

Liu, Gang; Yuan, Dan; Wang, Qi; Li, Wanrong; Cai, Jie; Li, Shuyi; Lamikanra, Olusola; Qin, Xinguang

2018-04-25

Egg white proteins are an excellent source of nutrition, with high biological and technological values. However, their limited functional properties prevent their widespread industrial applications. In this study, the ovalbumin functionality was improved via glycation by Maillard reaction with d-lactose. The free amino groups and sodium dodecyl sulfate-polyacrylamide gel electrophoresis profile were determined, confirming that glycation occurred between ovalbumin and lactose. The emulsification of the conjugate was 2.69-fold higher than that of ovalbumin at pH 7.0 after glycation. The thermal stability also improved remarkably. The glycated protein products were used to form an oil-water nanoemulsion for polymethoxyflavone-rich aged orange peel oil. The resulting nanoemulsion showed good pH, thermal, and storage stabilities.

Efficacy of the combined application of chitosan and Locust Bean Gum with different citrus essential oils to control postharvest spoilage caused by Aspergillus flavus in dates.

PubMed

Aloui, Hajer; Khwaldia, Khaoula; Licciardello, Fabio; Mazzaglia, Agata; Muratore, Giuseppe; Hamdi, Moktar; Restuccia, Cristina

2014-01-17

This study reports the efficacy of the combined application of chitosan (CH) and Locust Bean Gum (LBG) in combination with different citrus essential oils (EOs) to inhibit Aspergillus flavus in vitro and on artificially infected dates for a storage period of 12 days. The effect of these treatments on the fruits' sensory characteristics was evaluated to verify the complete absence of off-odours and off-flavours. Bergamot EO was the most effective in reducing mycelial growth, followed by bitter orange EO. Both bergamot and bitter orange oils significantly reduced conidial germination and a complete inhibition was obtained at concentrations higher than 2%. The mixtures based on CH-2% (v/v) bergamot EO or CH-2% (v/v) bitter orange EO proved to be the most effective coatings to reduce conidial germination resulting in an 87-90% inhibition compared with the control. In fruit decay assays coatings based on CH incorporating citrus oils were able to reduce fungal decay in the range of 52-62% at day 12. The study results and the complete absence of off-flavours and off-odours demonstrate the potential of CH coatings carrying citrus EOs at sub-inhibitory concentrations to control postharvest growth of A. flavus in dates. Copyright © 2013 Elsevier B.V. All rights reserved.

Thermal-Diffusivity Measurements of Mexican Citrus Essential Oils Using Photoacoustic Methodology in the Transmission Configuration

NASA Astrophysics Data System (ADS)

Muñoz, G. A. López; González, R. F. López; López, J. A. Balderas; Martínez-Pérez, L.

2011-05-01

Photoacoustic methodology in the transmission configuration (PMTC) was used to study the thermophysical properties and their relation with the composition in Mexican citrus essential oils providing the viability of using photothermal techniques for quality control and for authentication of oils and their adulteration. Linear relations for the amplitude (on a semi-log scale) and phase, as functions of the sample's thickness, for the PMTC was obtained through a theoretical model fit to the experimental data for thermal-diffusivity measurements in Mexican orange, pink grapefruit, mandarin, lime type A, centrifuged essential oils, and Mexican distilled lime essential oil. Gas chromatography for distilled lime essential oil and centrifuged lime essential oil type A is reported to complement the study. Experimental results showed close thermal-diffusivity values between Mexican citrus essential oils obtained by centrifugation, but a significant difference of this physical property for distilled lime oil and the corresponding value obtained by centrifugation, which is due to their different chemical compositions involved with the extraction processes.

Orange County Intelligent Vehicle/Highway Systems Study, Draft Final Report

DOT National Transportation Integrated Search

1993-06-01

THIS DOCUMENT REPRESENTS THE FINAL REPORT FOR THE ORANGE COUNTY INTELLIGENT VEHICLE-HIGHWAY SYSTEMS (IVHS) STUDY, PREPARED FOR THE ORANGE COUNTY TRANSPORTATION AUTHORITY (OCTA). THE PURPOSE OF THIS REPORT IS TO DOCUMENT THE FINDINGS OF THE IVHS STUDY...

Study of flavour compounds from orange juices by HS-SPME and GC-MS

NASA Astrophysics Data System (ADS)

Schmutzer, G.; Avram, V.; Covaciu, F.; Feher, I.; Magdas, A.; David, L.; Moldovan, Z.

2013-11-01

The flavour of the orange juices, which gives the taste and odour of the product, is an important criterion about the products quality for consumers. A fresh single strength and two commercial orange juices (obtained from concentrate) flavour profile were studied using a selective and sensitive gas chromatography - mass spectrometry (GC-MS) analytical system, after a solvent free, single step preconcentration and extraction technique, the headspace solid phase microextraction (HP-SPME). In the studied orange juices 55 flavour compounds were detected and classified as belonging to the esters, alcohols, ketones, monoterpenes and sesquiterpenes chemical families. The fresh single strength orange juice was characterized by high amount of esters, monoterpenes and sesquiterpenes. Limonene and valencene were the most abundant flavours in this fresh natural orange juice. Alcohols and ketones were found in higher concentration in the commercial orange juices made from concentrate, than in the single strength products. Nevertheless, in commercial juices the most abundant flavour was limonene and α-terpineol. The results highlight clear differences between fresh singles strength orange juice and juice from concentrate. The orange juices reconstructed from concentrate, made in Romania, present low quantity of flavour compounds, suggesting the absence or a low rearomatization process, but extraneous components were not detected.

Study of the absorption spectra of Fricke Xylenol Orange gel dosimeters

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gambarini, Grazia; Artuso, Emanuele; Liosi, Giulia Maria

2015-07-01

A systematic study of the absorption spectra of Fricke Xylenol Orange gel dosimeters has been performed, in the wavelength range from 300 nm to 700 nm. The spectrum of Xylenol Orange (without ferrous sulphate solution) has been achieved, in order to subtract its contribution from the absorption spectra of the irradiated Fricke Xylenol Orange gel dosimeters. The absorbance due to ferric ions chelated by Xylenol Orange has been studied for various irradiation doses. Two absorbance peaks are visible, mainly at low doses: the first peak increases with the dose more slowly than the second one. This effect can explain themore » apparent threshold dose that was frequently evidenced. (authors)« less

Synthesis and Luminescence Properties of Orange-Red Phosphors Na Y (WO4)2: Eu3+, Sm3+

NASA Astrophysics Data System (ADS)

Yu, Hong; Chen, Shanyong; Chen, Jinlei

2017-12-01

Orange-red phosphors NaY(WO4)2:Eu3+, Sm3+ were successfully synthesised by hydrothermal synthesis method. The photoluminescence (PL) properties were investigated. The PL emission spectrum of Na Y (WO4)2: Eu3+ shows good luminous properties under 398 nm, which mainly located at 596 and 615 nm, with the additional of Sm3+ could broaden and increase emission intensity of Eu3+, which could be suitable for the excitation of UV light emitting diodes (LEDs) (∼400 nm). The PL results indicated that Na Y (WO4)2: Eu3+, Sm3+ may be a promising red phosphor candidate for use in white LEDs.

Antioxidant effectiveness of organically and non-organically grown red oranges in cell culture systems.

PubMed

Tarozzi, A; Hrelia, S; Angeloni, C; Morroni, F; Biagi, P; Guardigli, M; Cantelli-Forti, G; Hrelia, P

2006-03-01

Consumers consider plant food products from organic origin healthier than the corresponding conventional plant foods. Clear experimental evidence supporting this assumption is still lacking. To determine if the organic red oranges have a higher phyto-chemical content (i. e., phenolics, anthocyanins and ascorbic acid), total antioxidant activity and in vitro bioactivity, in terms of protective effect against oxidative damage at cellular level, than nonorganic red oranges. Total phenolics were measured using the Folin Ciocalteau assay, while total anthocyanins and ascorbic acid levels were determined by spectrophotometric and HPLC analysis, respectively. In addition, the total antioxidant activity of red orange extracts was measured by the ABTS(*+) test. The ability of red orange extracts to counteract conjugated diene containing lipids and free radical production in cultured rat cardiomyocytes and differentiated Caco-2 cells, respectively, was assessed. Organic oranges had significantly higher total phenolics, total anthocyanins and ascorbic acid levels than the corresponding non-organic oranges (all p < 0.05). Moreover, the organic orange extracts had a higher total antioxidant activity than non-organic orange extracts (p < 0.05). In addition, our results indicate that red oranges have a strong capacity of inhibiting the production of conjugated diene containing lipids and free radicals in rat cardiomyocytes and differentiated Caco-2 cells, respectively. Statistically higher levels of antioxidant activity in both cell models were found in organically grown oranges as compared to those produced by integrated agriculture practice. Our results clearly show that organic red oranges have a higher phytochemical content (i. e., phenolics, anthocyanins and ascorbic acid), total antioxidant activity and bioactivity than integrated red oranges. Further studies are needed to confirm whether the organic agriculture practice is likely to increase the antioxidant activity of

Bio-refinery of orange peels waste: a new concept based on integrated green and solvent free extraction processes using ultrasound and microwave techniques to obtain essential oil, polyphenols and pectin.

PubMed

Boukroufa, Meryem; Boutekedjiret, Chahrazed; Petigny, Loïc; Rakotomanomana, Njara; Chemat, Farid

2015-05-01

In this study, extraction of essential oil, polyphenols and pectin from orange peel has been optimized using microwave and ultrasound technology without adding any solvent but only "in situ" water which was recycled and used as solvent. The essential oil extraction performed by Microwave Hydrodiffusion and Gravity (MHG) was optimized and compared to steam distillation extraction (SD). No significant changes in yield were noticed: 4.22 ± 0.03% and 4.16 ± 0.05% for MHG and SD, respectively. After extraction of essential oil, residual water of plant obtained after MHG extraction was used as solvent for polyphenols and pectin extraction from MHG residues. Polyphenols extraction was performed by ultrasound-assisted extraction (UAE) and conventional extraction (CE). Response surface methodology (RSM) using central composite designs (CCD) approach was launched to investigate the influence of process variables on the ultrasound-assisted extraction (UAE). The statistical analysis revealed that the optimized conditions of ultrasound power and temperature were 0.956 W/cm(2) and 59.83°C giving a polyphenol yield of 50.02 mgGA/100 g dm. Compared with the conventional extraction (CE), the UAE gave an increase of 30% in TPC yield. Pectin was extracted by conventional and microwave assisted extraction. This technique gives a maximal yield of 24.2% for microwave power of 500 W in only 3 min whereas conventional extraction gives 18.32% in 120 min. Combination of microwave, ultrasound and the recycled "in situ" water of citrus peels allow us to obtain high added values compounds in shorter time and managed to make a closed loop using only natural resources provided by the plant which makes the whole process intensified in term of time and energy saving, cleanliness and reduced waste water. Copyright © 2014 Elsevier B.V. All rights reserved.

Alcoholic fermentation induces melatonin synthesis in orange juice.

PubMed

Fernández-Pachón, M S; Medina, S; Herrero-Martín, G; Cerrillo, I; Berná, G; Escudero-López, B; Ferreres, F; Martín, F; García-Parrilla, M C; Gil-Izquierdo, A

2014-01-01

Melatonin (N-acetyl-5-methoxytryptamine) is a molecule implicated in multiple biological functions. Its level decreases with age, and the intake of foods rich in melatonin has been considered an exogenous source of this important agent. Orange is a natural source of melatonin. Melatonin synthesis occurs during alcoholic fermentation of grapes, malt and pomegranate. The amino acid tryptophan is the precursor of all 5-methoxytryptamines. Indeed, melatonin appears in a shorter time in wines when tryptophan is added before fermentation. The aim of the study was to measure melatonin content during alcoholic fermentation of orange juice and to evaluate the role of the precursor tryptophan. Identification and quantification of melatonin during the alcoholic fermentation of orange juice was carried out by UHPLC-QqQ-MS/MS. Melatonin significantly increased throughout fermentation from day 0 (3.15 ng/mL) until day 15 (21.80 ng/mL) reaching larger amounts with respect to other foods. Melatonin isomer was also analysed, but its content remained stable ranging from 11.59 to 14.18 ng/mL. The enhancement of melatonin occurred mainly in the soluble fraction. Tryptophan levels significantly dropped from 13.80 mg/L (day 0) up to 3.19 mg/L (day 15) during fermentation. Melatonin was inversely and significantly correlated with tryptophan (r = 0.907). Therefore, the enhancement in melatonin could be due to both the occurrence of tryptophan and the new synthesis by yeast. In summary, the enhancement of melatonin in novel fermented orange beverage would improve the health benefits of orange juice by increasing this bioactive compound. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Effects of photochemical smog on lemons and navel oranges

DOE Office of Scientific and Technical Information (OSTI.GOV)

Thompson, C.R.; Taylor, O.C.; Richards, B.L.

1970-05-01

Experiments were carried out on lemon and Navel orange trees to determine the kind and extent of damage caused by air pollution in the Los Angeles Basin. Trees were enclosed in filter-equipped greenhouses and compared with control trees which were exposed to ambient air. Yield of lemons was about one third more in filtered trees, and with oranges, the yield was about doubled. Fruit drop in oranges was a major problem associated with the exposure to ambient air pollution. Trees in ambient air were reduced in photosynthesis to 66% of filtered-air treatments. Fluoride, while present in the atmosphere was ofmore » minor importance to the health and performance of the trees. 2 tables.« less

A Saponification Method for Chlorophyll Removal from Microalgae Biomass as Oil Feedstock

PubMed Central

Li, Tao; Xu, Jin; Wu, Hualian; Wang, Guanghua; Dai, Shikun; Fan, Jiewei; He, Hui; Xiang, Wenzhou

2016-01-01

Microalgae oil is an optimal feedstock for nutraceutical, pharmaceutical and biodiesel production, but its high levels of chlorophyll limit its large-scale application. To date, few effective approaches have been developed to remove chlorophyll from microalgae oil. The main purpose of this study was to present a preprocessing method of algae oil feedstock (Scenedesmus) to remove chlorophyll by saponification. The results showed that 96% of chlorophyll in biomass was removed. High quality orange transparent oil could be extracted from the chlorophyll reduced biomass. Specifically, the proportion of neutral lipids and saturation levels of fatty acids increased, and the pigments composition became carotenoids-based. The critical parameters of chlorophyll reduced biodiesel conformed to the standards of the USA, China and EU. Sodium copper chlorophyllin could be prepared from the bleaching effluent. The results presented herein offer a useful pathway to improve the quality of microalgae oil and reduce the cost of microalgae biodiesel. PMID:27618070

A Saponification Method for Chlorophyll Removal from Microalgae Biomass as Oil Feedstock.

PubMed

Li, Tao; Xu, Jin; Wu, Hualian; Wang, Guanghua; Dai, Shikun; Fan, Jiewei; He, Hui; Xiang, Wenzhou

2016-09-07

Microalgae oil is an optimal feedstock for nutraceutical, pharmaceutical and biodiesel production, but its high levels of chlorophyll limit its large-scale application. To date, few effective approaches have been developed to remove chlorophyll from microalgae oil. The main purpose of this study was to present a preprocessing method of algae oil feedstock (Scenedesmus) to remove chlorophyll by saponification. The results showed that 96% of chlorophyll in biomass was removed. High quality orange transparent oil could be extracted from the chlorophyll reduced biomass. Specifically, the proportion of neutral lipids and saturation levels of fatty acids increased, and the pigments composition became carotenoids-based. The critical parameters of chlorophyll reduced biodiesel conformed to the standards of the USA, China and EU. Sodium copper chlorophyllin could be prepared from the bleaching effluent. The results presented herein offer a useful pathway to improve the quality of microalgae oil and reduce the cost of microalgae biodiesel.

Orange juice substantially reduces the bioavailability of the beta-adrenergic-blocking agent celiprolol.

PubMed

Lilja, Jari J; Juntti-Patinen, Laura; Neuvonen, Pertti J

2004-03-01

Grapefruit juice was recently found to decrease plasma concentrations of the beta-adrenergic receptor-blocking agent celiprolol. Our objective was to investigate the effect of orange juice on the pharmacokinetics of celiprolol in healthy subjects. In a randomized crossover study with 2 phases and a washout of 2 weeks, 10 healthy volunteers ingested either 200 mL normal-strength orange juice or water 3 times a day for 2 days. On the morning of day 3, 1 hour after ingestion of 200 mL orange juice or water, each subject ingested 100 mg celiprolol with either 200 mL orange juice or water. In addition, 200 mL orange juice or water was ingested at 4, 10, 22, and 27 hours after celiprolol intake. The concentrations of celiprolol in plasma and its excretion into urine were measured up to 33 hours after its dosing. Systolic and diastolic blood pressures and heart rate were recorded up to 10 hours. Orange juice reduced the mean peak plasma concentration of celiprolol by 89% (P <.01) and the mean area under the plasma celiprolol concentration-time curve by 83% (P <.01). The time to peak concentration of celiprolol increased from 4 to 6 hours (P <.05), and the half-life was prolonged from 4.6 to 10.8 hours (P =.05) after ingestion of orange juice. Orange juice reduced the urinary excretion of celiprolol by 77% (P <.01). No significant differences were observed in the hemodynamic variables between the phases. Orange juice substantially reduces the bioavailability of celiprolol, but the mechanism of this interaction remains to be resolved. For example, modulation of intestinal pH and of function of transporters implicated in the absorption of celiprolol may be involved. Because of the great extent of the orange juice-celiprolol interaction and a wide use of orange juice, this interaction is likely to have clinical importance in some patients, although hemodynamic consequences were not seen in young healthy subjects.

21 CFR 74.1260 - D&C Orange No. 10.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 1 2011-04-01 2011-04-01 false D&C Orange No. 10. 74.1260 Section 74.1260 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR ADDITIVES SUBJECT TO CERTIFICATION Drugs § 74.1260 D&C Orange No. 10. (a) Identity. (1) The color...

21 CFR 74.1254 - D&C Orange No. 4.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 1 2011-04-01 2011-04-01 false D&C Orange No. 4. 74.1254 Section 74.1254 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR ADDITIVES SUBJECT TO CERTIFICATION Drugs § 74.1254 D&C Orange No. 4. (a) Identity. (1) the color additive D...

21 CFR 74.1261 - D&C Orange No. 11.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 1 2011-04-01 2011-04-01 false D&C Orange No. 11. 74.1261 Section 74.1261 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR ADDITIVES SUBJECT TO CERTIFICATION Drugs § 74.1261 D&C Orange No. 11. (a) Identity. (1) The color...

21 CFR 74.1255 - D&C Orange No. 5.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 1 2011-04-01 2011-04-01 false D&C Orange No. 5. 74.1255 Section 74.1255 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR ADDITIVES SUBJECT TO CERTIFICATION Drugs § 74.1255 D&C Orange No. 5. (a) Identity. (1) the color additive D...

21 CFR 73.3112 - C.I. Vat Orange 1.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 1 2010-04-01 2010-04-01 false C.I. Vat Orange 1. 73.3112 Section 73.3112 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR ADDITIVES EXEMPT FROM CERTIFICATION Medical Devices § 73.3112 C.I. Vat Orange 1. (a) Identity. The...

21 CFR 73.3112 - C.I. Vat Orange 1.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 21 Food and Drugs 1 2012-04-01 2012-04-01 false C.I. Vat Orange 1. 73.3112 Section 73.3112 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR ADDITIVES EXEMPT FROM CERTIFICATION Medical Devices § 73.3112 C.I. Vat Orange 1. (a) Identity. The...

21 CFR 73.3112 - C.I. Vat Orange 1.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 21 Food and Drugs 1 2014-04-01 2014-04-01 false C.I. Vat Orange 1. 73.3112 Section 73.3112 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR ADDITIVES EXEMPT FROM CERTIFICATION Medical Devices § 73.3112 C.I. Vat Orange 1. (a) Identity. The...

21 CFR 73.3112 - C.I. Vat Orange 1.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 1 2011-04-01 2011-04-01 false C.I. Vat Orange 1. 73.3112 Section 73.3112 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR ADDITIVES EXEMPT FROM CERTIFICATION Medical Devices § 73.3112 C.I. Vat Orange 1. (a) Identity. The...

21 CFR 73.3112 - C.I. Vat Orange 1.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 21 Food and Drugs 1 2013-04-01 2013-04-01 false C.I. Vat Orange 1. 73.3112 Section 73.3112 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR ADDITIVES EXEMPT FROM CERTIFICATION Medical Devices § 73.3112 C.I. Vat Orange 1. (a) Identity. The...

21 CFR 82.1255 - D&C Orange No. 5.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 1 2010-04-01 2010-04-01 false D&C Orange No. 5. 82.1255 Section 82.1255 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF CERTIFIED...) The color additive D&C Orange No. 5. may be safely used for coloring externally applied drugs in...

21 CFR 82.1255 - D&C Orange No. 5.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 1 2011-04-01 2011-04-01 false D&C Orange No. 5. 82.1255 Section 82.1255 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF CERTIFIED...) The color additive D&C Orange No. 5. may be safely used for coloring externally applied drugs in...

Photoluminescence Properties of Ca3Si2O7: Pr3+ Orange-Red Phosphors Prepared by High-Temperature Solid-State Method

NASA Astrophysics Data System (ADS)

Peng, Zhi-Qing; Chen, Rong; Feng, Wen-Lin

2018-06-01

Novel luminescent materials Ca3-xSi2O7: xPr3+ were successfully prepared by the high-temperature solid-state method. The crystal structure, morphology, and optical spectrum were characterised by X-ray diffraction (XRD), scanning electron microscopy (SEM), and spectroscopy, respectively. The XRD patterns of the samples indicate that the crystal structure is monoclinic symmetry. The SEM shows that the selected sample has good crystallinity although its appearance is irregular and scalelike. The peak of the excitation spectrum of the sample is located at around 449 nm, corresponding to 3H4→3P2 transition of Pr3+. The peak of the emission spectrum of the sample is situated at around 612 nm which is attributed to 3P0→3H6 transition of Pr3+, and the colour is orange-red. The optimum concentration for Pr3+ replaced Ca2+ sites in Ca3Si2O7: Pr3+ is 0.75 mol%. The lifetime (8.48 μs) of a typical sample (Ca2.9925Pr0.0075)Si2O7 is obtained. It reveals that orange-red phosphors Ca3-xSi2O7: xPr3+ possess remarkable optical properties and can be used in white light emitting devices.

Rapid discrimination of bergamot essential oil by paper spray mass spectrometry and chemometric analysis.

PubMed

Taverna, Domenico; Di Donna, Leonardo; Mazzotti, Fabio; Tagarelli, Antonio; Napoli, Anna; Furia, Emilia; Sindona, Giovanni

2016-09-01

A novel approach for the rapid discrimination of bergamot essential oil from other citrus fruits oils is presented. The method was developed using paper spray mass spectrometry (PS-MS) allowing for a rapid molecular profiling coupled with a statistic tool for a precise and reliable discrimination between the bergamot complex matrix and other similar matrices, commonly used for its reconstitution. Ambient mass spectrometry possesses the ability to record mass spectra of ordinary samples, in their native environment, without sample preparation or pre-separation by creating ions outside the instrument. The present study reports a PS-MS method for the determination of oxygen heterocyclic compounds such as furocoumarins, psoralens and flavonoids present in the non-volatile fraction of citrus fruits essential oils followed by chemometric analysis. The volatile fraction of Bergamot is one of the most known and fashionable natural products, which found applications in flavoring industry as ingredient in beverages and flavored foodstuff. The development of the presented method employed bergamot, sweet orange, orange, cedar, grapefruit and mandarin essential oils. PS-MS measurements were carried out in full scan mode for a total run time of 2 min. The capability of PS-MS profiling to act as marker for the classification of bergamot essential oils was evaluated by using multivariate statistical analysis. Two pattern recognition techniques, linear discriminant analysis and soft independent modeling of class analogy, were applied to MS data. The cross-validation procedure has shown excellent results in terms of the prediction ability because both models have correctly classified all samples for each category. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

Vietnamese Victims of Agent Orange and U.S.-Vietnam Relations

DTIC Science & Technology

2009-05-28

Hercules Inc., Monsanto Company, T-H Agricultural & Nutrition Company, Thompson Chemicals Corporation, and Uniroyal Inc. Agent Orange was one of 15...ban the import of agricultural and aquatic goods from Vietnam for health reasons. 85 Tom Fawthrop, “Agent Orange Victims Sue Monsanto ,” Corpwatch

[Exposure to nitrogen dioxide and respiratory health at 2 years in the INMA-Valencia cohort].

PubMed

Gutiérrez Oyarce, Alejandra; Ferrero, Amparo; Estarlich, Marisa; Esplugues, Ana; Iñiguez, Carmen; Ballester, Ferran

2017-07-25

To analyze the association between exposure to nitrogen dioxide (NO 2 ) during pregnancy and the postnatal period up to the age of 2 years old and the incidence of respiratory problems in children from the INMA-Valencia cohort. The study population included 624 children from the INMA-Valencia cohort. Individual exposure to NO 2 was estimated in different environments outside the home during pregnancy and up to the age of 2 using empirical measurement and data from geo-statistical methods. Respiratory symptoms were obtained from a questionnaire applied at the age of two. The association between NO 2 exposure and respiratory symptoms was performed using multivariate logistic regression. The cumulative incidence was 16.3% for persistent cough, 34.9% for wheezing and 27.6% for lower respiratory tract infections. No association was found between respiratory symptoms and exposure to NO 2 in any of the children. However an association between NO 2 exposure and persistent cough was found at two years of life in the children with a parental history of allergy. NO 2 exposure would lead to persistent cough in children with a parental history of allergies. Copyright © 2017 SESPAS. Publicado por Elsevier España, S.L.U. All rights reserved.

ORANGES evaluation discussion group process

DOT National Transportation Integrated Search

2003-03-24

This report describes and documents the process and methodology to be used to conduct a series of before and after discussion groups with smart card holders and participating agency employees as part of the ORANGES multiple purpose smart card Field O...

Repellent Activity of Botanical Oils against Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Liviidae).

PubMed

Kuhns, Emily H; Martini, Xavier; Hoyte, Angel; Stelinski, Lukasz L

2016-07-14

The Asian citrus psyllid, Diaphorina citri Kuwayama, is the insect vector of the pathogen causing huanglongbing. We selected three botanical oils to evaluate behavioral activity against D. citri. In laboratory olfactometer assays, fir oil was repellent to D. citri females, while litsea and citronella oils elicited no response from D. citri females. In choice settling experiments, D. citri settled almost completely on control plants rather than on plants treated with fir oil at a 9.5 mg/day release rate. Therefore, we conducted field trials to determine if fir oil reduced D. citri densities in citrus groves. We found no repellency of D. citri from sweet orange resets that were treated with fir oil dispensers releasing 10.4 g/day/tree as compared with control plots. However, we found a two-week decrease in populations of D. citri as compared with controls when the deployment rate of these dispensers was doubled. Our results suggest that treatment of citrus with fir oil may have limited activity as a stand-alone management tool for D. citri and would require integration with other management practices.

Synthesis and Optical Spectroscopy of YPO4:Eu3+ Orange-Red Phosphors

NASA Astrophysics Data System (ADS)

Yahiaoui, Z.; Hassairi, M. A.; Dammak, M.

2017-08-01

YPO4: x mol.% Eu3+ phosphors with different dopant concentrations ( x = 3, 5, 8, 11, 13) have been synthesized via high-temperature solid-state reaction. X-ray diffraction analysis and Raman and infrared (IR) spectroscopy were applied for detailed structural characterization. Under excitation at wavelength of 395 nm, the photoluminescence spectra displayed the 5D0 → 7F J ( J = 1, 2, 3, 4) intra-4 f shell transitions related to Eu3+ ion. The radiative lifetime was estimated using the Ω 2 and Ω 4 Judd-Ofelt intensity parameters. The highest luminescence intensity was achieved for an optimal europium concentration of 11 mol.%. The critical energy-transfer distance for Eu3+ ions was evaluated to be 10.74 Å. We also studied the temperature-dependent photoluminescence and Commission Internationale de l'Éclairage chromaticity diagram. It was found that Eu3+-doped YPO4 exhibited good thermal stability and its emission intensity decreased slightly above room temperature. In addition, the color purity of this phosphor was as high as 91% for the YPO4:13% Eu3+ sample, making it a potential orange-red phosphor for application in ultraviolet-pumped white light-emitting diodes.

Educating the Orang Asli Children: Exploring Indigenous Children's Practices and Experiences in Schools

ERIC Educational Resources Information Center

Renganathan, Sumathi

2016-01-01

The author is concerned with the education available for the Orang Asli, an indigenous minority community in Malaysia. Literature written about Orang Asli and education mostly assumes a deficit perspective where the lack of educational achievement among the Orang Asli children is often attributed to their culture and community. Therefore, rather…

75 FR 55968 - Special Local Regulations, Sabine River; Orange, TX

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-15

...-AA08 Special Local Regulations, Sabine River; Orange, TX AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary Special Local Regulation in the Port Arthur Captain of the Port Zone on the Sabine River, Orange, Texas. This Special Local Regulation is...

Aroma Leakage from Orange Juice Packed in Gable-Top Paper Containers for Chilled Distribution.

PubMed

Aoki, Risa; Tokuda, Aika; Shigemura, Yasutaka; Mineki, Machiko; Sato, Yoshio

2017-01-01

We conducted a study to examine aroma leakage from orange juice packed in gable-top paper containers for chilled distribution. Limonene, an aromatic component of orange juice, was considered as an index compound of aroma leakage, and its seepage on the surface of the container and concentration in the orange juice were measured by GC-MS for 12 commercial samples. After 3 days of storage, limonene was detected on the surface of 8 orange juice containers, and the concentration of limonene in the orange juice was found to have decreased. Thus, limonene leaked through the container within a few days, and the extent of leakage differed between containers, presumably depending upon their barrier properties. In addition, limonene was detected in green tea and milk that was stored together with the unopened orange juice containers at 4℃. The transference of orange aroma into milk was significant, because the contamination of the milk was confirmed by subjective sensory evaluation. This study suggests the possibility of transfer of aroma compounds through paper containers to other beverages.

[Prevalence of oral anticoagulation and quality of its management in primary healthcare: A study by the Health Sentinel Network of the Region of Valencia (Spain)].

PubMed

Boned-Ombuena, Ana; Pérez-Panadés, Jordi; López-Maside, Aurora; Miralles-Espí, Maite; Guardiola Vilarroig, Sandra; Adam Ruiz, Desamparados; Zurriaga, Oscar

2017-11-01

To estimate the prevalence of patients with oral anticoagulant therapy (OAT) in the Region of Valencia and to evaluate the quality of management of OAT with vitaminK antagonists (VKA) carried out in primary healthcare. Observational cross-sectional study conducted through the Health Sentinel Network of the Region of Valencia, which includes a survey and the retrospective analysis of OAT monitoring. Primary healthcare, Region of Valencia, Spain. All patients aged 18years or older on OAT who consulted during the year 2014. The population covered by the 59 doctors of the Health Sentinel Network constitutes 2.2% of the adult population of the Region of Valencia, and it is representative of it. Demographic, socioeconomic and health data as well as information concerning OAT. Quality of OAT management with VKA was assessed by means of the percentage of time in therapeutic range (TTR), computed using the Rosendaal method. A total of 1,144 patients were recorded (mean age 74.5±11 years; 49.7% women). Prevalence of OAT in the Region of Valencia is 1.3 cases per 100 population. The characteristic profile of these patients is an old person, with several comorbidities and a low level of education, who lives accompanied. Atrial fibrillation is the most common indication. 82.8% of patients on OAT with VKA were monitored in primary healthcare. The average TTR was 65.0%, and 53.9% of patients had a TTR ≥65%. Among inadequately controlled patients, 74.4% were perceived as well-controlled by their primary care doctor. Prevalence of OAT is high, and it is expected to increase. The degree of control achieved meets the generally accepted quality standard (mean TTR ≥65%), and it is comparable to that observed in other national and international studies. However, there is wide scope for improvement. It is crucial to optimize the management of this therapy in the most effective and cost-effective way. Among other measures, access of physicians to their patients' clinical information

Variability of Carotenoid Biosynthesis in Orange Colored Capsicum spp.

PubMed Central

Guzman, Ivette; Hamby, Shane; Romero, Joslynn; Bosland, Paul W.; O’Connell, Mary A.

2010-01-01

Pepper, Capsicum spp., is a worldwide crop valued for heat, nutrition, and rich pigment content. Carotenoids, the largest group of plant pigments, function as antioxidants and as vitamin A precursors. The most abundant carotenoids in ripe pepper fruits are β-carotene, capsanthin, and capsorubin. In this study, the carotenoid composition of orange fruited Capsicum lines was defined along with the allelic variability of the biosynthetic enzymes. The carotenoid chemical profiles present in seven orange pepper varieties were determined using a novel UPLC method. The orange appearance of the fruit was due either to the accumulation of β-carotene, or in two cases, due to only the accumulation of red and yellow carotenoids. Four carotenoid biosynthetic genes, Psy, Lcyb, CrtZ-2, and Ccs were cloned and sequenced from these cultivars. This data tested the hypothesis that different alleles for specific carotenoid biosynthetic enzymes are associated with specific carotenoid profiles in orange peppers. While the coding regions within Psy and CrtZ-2 did not change in any of the lines, the genomic sequence contained introns not previously reported. Lcyb and Ccs contained no introns but did exhibit polymorphisms resulting in amino acid changes; a new Ccs variant was found. When selectively breeding for high provitamin A levels, phenotypic recurrent selection based on fruit color is not sufficient, carotenoid chemical composition should also be conducted. Based on these results, specific alleles are candidate molecular markers for selection of orange pepper lines with high β-carotene and therefore high pro-vitamin A levels. PMID:20582146

Purple is the new Orange

USDA-ARS?s Scientific Manuscript database

Blood orange and Cara cara-like citrus varieties with purple or red fruit color, increased antioxidants and modified flavor could be the next generation of cultivars produced via genetic engineering. These varieties are being developed by enhancing the presence of anthocyanin and lycopene pigments...

The CaO orange system in meteor spectra

NASA Astrophysics Data System (ADS)

Berezhnoy, A. A.; Borovička, J.; Santos, J.; Rivas-Silva, J. F.; Sandoval, L.; Stolyarov, A. V.; Palma, A.

2018-02-01

The CaO orange band system was simulated in the region 5900-6300 Å and compared with the experimentally observed spectra of Benešov bolide wake. The required vibronic Einstein emission coefficients were estimated by means of the experimental radiative lifetimes under the simplest Franck-Condon approximation. A moderate agreement was achieved, and the largest uncertainties come from modeling shape of FeO orange bands. Using a simple model the CaO column density in the wake of the Benešov bolide at the height of 29 km was estimated as (5 ± 2) × 1014 cm-2 by a comparison of the present CaO spectra with the AlO bands nicely observed at 4600-5200 Å in the same spectrum. The obtained CaO content is in a good agreement with the quenching model developed for the impact-produced cloud, although future theoretical and experimental studies of both CaO and FeO orange systems contribution would be needed to confirm these results.

Absence of furanocoumarins in Advantra Z® (Citrus aurantium, bitter orange) extracts.

PubMed

Stohs, Sidney J; Miller, Howard; Romano, Felice

2014-09-01

Grapefruit (Citrus paradisi) juice is known for its ability to alter drug metabolism through inhibition of the cytochrome P450-3A4 (CYP3A4) system, and result in drug-food interactions that may be life threatening. The primary active ingredients in grapefruit responsible for these effects are the furanocoumarins bergapten, bergamottin, and 6',7'-dihydroxybergamottin (DHB). Bergamottin and DHB appear to be the most important in terms of adverse drug interactions. Furanocoumarins are present in the juices and fruits of other Citrus species including C. aurantium (bitter oranges). Bergapten is the predominant furanocoumarin in bitter orange. Bitter orange extracts are widely used in products associated with weight loss, sports performance, and energy production. Questions have been raised about the potential of bitter orange extracts to cause drug interactions. This study examined the furanocoumarin content of four standardized bitter orange extracts (Advantra Z®) by liquid chromatography-mass spectroscopy. The results indicated that the total furanocoumarin content of each of the four extracts was less than 20 μg/g, amounts insufficient to exert significant effects on the metabolism of susceptible drugs in human subjects at the doses commonly used for these extracts.

Tracking the degradation of fresh orange juice and discrimination of orange varieties: an example of NMR in coordination with chemometrics analyses.

PubMed

de Oliveira, Clayton R; Carneiro, Renato L; Ferreira, Antonio G

2014-12-01

Brazil is currently the largest exporter of concentrated orange juice and, unlike the other exporter countries, the domestic consumption is mainly based on the fresh orange juice. The quality control by evaluating the major chemical constituents under the influence of the most important factors, such as temperature and storage time of the product, is very important in this context. Therefore, the objective of this study was to evaluate the influence of temperature and time on the degradation of fresh orange juice for 24h, by using (1)H NMR technique and chemometric tools for data mining. The storage conditions at 24h led to the production of the formic, fumaric and acetic acids; and an increase of succinic and lactic acids and ethanol, which were observed at low concentration at the initial time. Furthermore, analysis by PCA has successfully distinguished the juice of different species/varieties as well as the metabolites responsible for their separation. Copyright © 2014 Elsevier Ltd. All rights reserved.

A review of Agent Orange and its associated oncologic risk of genitourinary cancers.

PubMed

Chang, Chrystal; Benson, Michael; Fam, Mina M

2017-11-01

Agent Orange is an herbicide sprayed widely in Vietnam that is linked to a variety of malignancies in as early as 1991.Since then, there has been concern for, and subsequent interest in studying, the potential connection between Agent Orange and other malignancies. In the past 2 decades, there have been significant changes in the opinion of the National Academy of Science regarding Agent Orange and certain genitourinary malignancies. Herein, we review the literature regarding the potential link between Agent Orange and various urological cancers, including prostate, bladder, testicular, and renal cancers. Copyright © 2017 Elsevier Inc. All rights reserved.

Metabolic engineering of β-carotene in orange fruit increases its in vivo antioxidant properties.

PubMed

Pons, Elsa; Alquézar, Berta; Rodríguez, Ana; Martorell, Patricia; Genovés, Salvador; Ramón, Daniel; Rodrigo, María Jesús; Zacarías, Lorenzo; Peña, Leandro

2014-01-01

Orange is a major crop and an important source of health-promoting bioactive compounds. Increasing the levels of specific antioxidants in orange fruit through metabolic engineering could strengthen the fruit's health benefits. In this work, we have afforded enhancing the β-carotene content of orange fruit through blocking by RNA interference the expression of an endogenous β-carotene hydroxylase gene (Csβ-CHX) that is involved in the conversion of β-carotene into xanthophylls. Additionally, we have simultaneously overexpressed a key regulator gene of flowering transition, the FLOWERING LOCUS T from sweet orange (CsFT), in the transgenic juvenile plants, which allowed us to obtain fruit in an extremely short period of time. Silencing the Csβ-CHX gene resulted in oranges with a deep yellow ('golden') phenotype and significant increases (up to 36-fold) in β-carotene content in the pulp. The capacity of β-carotene-enriched oranges for protection against oxidative stress in vivo was assessed using Caenorhabditis elegans as experimental animal model. Golden oranges induced a 20% higher antioxidant effect than the isogenic control. This is the first example of the successful metabolic engineering of the β-carotene content (or the content of any other phytonutrient) in oranges and demonstrates the potential of genetic engineering for the nutritional enhancement of fruit tree crops. © 2013 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

21 CFR 146.148 - Reduced acid frozen concentrated orange juice.

Code of Federal Regulations, 2014 CFR

2014-04-01

... that the ratio of the Brix reading to the grams of acid, expressed as anhydrous citric acid, per 100... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Reduced acid frozen concentrated orange juice. 146... Canned Fruit Juices and Beverages § 146.148 Reduced acid frozen concentrated orange juice. (a) Reduced...

21 CFR 146.148 - Reduced acid frozen concentrated orange juice.

Code of Federal Regulations, 2012 CFR

2012-04-01

... that the ratio of the Brix reading to the grams of acid, expressed as anhydrous citric acid, per 100... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Reduced acid frozen concentrated orange juice. 146... Canned Fruit Juices and Beverages § 146.148 Reduced acid frozen concentrated orange juice. (a) Reduced...

21 CFR 146.148 - Reduced acid frozen concentrated orange juice.

Code of Federal Regulations, 2011 CFR

2011-04-01

... that the ratio of the Brix reading to the grams of acid, expressed as anhydrous citric acid, per 100... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Reduced acid frozen concentrated orange juice. 146... Canned Fruit Juices and Beverages § 146.148 Reduced acid frozen concentrated orange juice. (a) Reduced...

21 CFR 146.148 - Reduced acid frozen concentrated orange juice.

Code of Federal Regulations, 2013 CFR

2013-04-01

... that the ratio of the Brix reading to the grams of acid, expressed as anhydrous citric acid, per 100... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Reduced acid frozen concentrated orange juice. 146... Canned Fruit Juices and Beverages § 146.148 Reduced acid frozen concentrated orange juice. (a) Reduced...

"In situ" extraction of essential oils by use of Dean-Stark glassware and a Vigreux column inside a microwave oven: a procedure for teaching green analytical chemistry.

PubMed

Chemat, Farid; Perino-Issartier, Sandrine; Petitcolas, Emmanuel; Fernandez, Xavier

2012-08-01

One of the principal objectives of sustainable and green processing development remains the dissemination and teaching of green chemistry in colleges, high schools, and academic laboratories. This paper describes simple glassware that illustrates the phenomenon of extraction in a conventional microwave oven as energy source and a process for green analytical chemistry. Simple glassware comprising a Dean-Stark apparatus (for extraction of aromatic plant material and recovery of essential oils and distilled water) and a Vigreux column (as an air-cooled condenser inside the microwave oven) was designed as an in-situ extraction vessel inside a microwave oven. The efficiency of this experiment was validated for extraction of essential oils from 30 g fresh orange peel, a by-product in the production of orange juice. Every laboratory throughout the world can use this equipment. The microwave power is 100 W and the irradiation time 15 min. The method is performed at atmospheric pressure without added solvent or water and furnishes essential oils similar to those obtained by conventional hydro or steam distillation. By use of GC-MS, 22 compounds in orange peel were separated and identified; the main compounds were limonene (72.1%), β-pinene (8.4%), and γ-terpinene (6.9%). This procedure is appropriate for the teaching laboratory, does not require any special microwave equipment, and enables the students to learn the skills of extraction, and chromatographic and spectroscopic analysis. They are also exposed to a dramatic visual example of rapid, sustainable, and green extraction of an essential oil, and are introduced to successful sustainable and green analytical chemistry.

Adverse Phototoxic Effect of Essential Plant Oils on NIH 3T3 Cell Line after UV Light Exposure.

PubMed

Binder, Svatopluk; Hanáková, Adéla; Tománková, Kateřina; Pížová, Klára; Bajgar, Robert; Manišová, Barbora; Kejlová, Kristina; Bendová, Hana; Jírová, Dagmar; Kolářová, Hana

2016-09-01

Natural or artificial substances have become an inseparable part of our lives. It is questionable whether adequate testing has been performed in order to ensure these substances do not pose a serious health risk. The principal aim of our research was to clarify the potential risk of adding essential oils to food, beverages and cosmetic products. The toxicity of substances frequently employed in cosmetics, aromatherapy and food industry (bergamot oil, Litsea cubeba oil, orange oil, citral) were investigated using cell line NIH3T3 (mouse fibroblasts) with/without UV irradiation. The MTT assay was used to estimate the cell viability. Reactive oxygen species (ROS) which are products of a number of natural cellular processes such as oxygen metabolism and inflammation were measured to determine the extent of cellular stress. DNA damage caused by strand breaks was examined by comet assay. MTT test determined EC50 values for all tested substances, varying from 0.0023% v/v for bergamot oil to 0.018% v/v for citral. ROS production measurement showed that UV radiation induces oxidative stress to the cell resulting in higher ROS production compared to the control and non-irradiated samples. Comet assay revealed that both groups (UV, without UV) exert irreversible DNA damage resulting in a cell death. Our findings suggest that even low concentrations (lower than 0.0464% v/v) of orange oil can be considered as phototoxic (PIF value 8.2) and probably phototoxic for bergamot oil (PIF value 4.6). We also found significant changes in the cell viability, the ROS production and the DNA after the cells were exposed to the tested chemicals. Even though these substances are widely used as antioxidants it should be noted that they present a risk factor and their use in cosmetic and food products should be minimized. Copyright© by the National Institute of Public Health, Prague 2016

The Valencia Scale of Attitudes and Beliefs Toward Hypnosis-Client version and hypnotizability.

PubMed

Green, Joseph P

2012-01-01

Abstract The present study examined responses on the Valencia Scale of Attitudes and Beliefs Toward Hypnosis-Client (VSABTH-C) version among a sample of American college students (N = 448) and explored the relationship between VSABTH-C factor scores and measures of hypnotizability, fantasy proneness, and absorption. Scores across three factors (i.e., help, interest, and marginal factors) accounted for 12% of the variance in responsiveness to suggestions administered from the Harvard Group Scale of Hypnotic Susceptibility, Form A. Normative data on the VSABTH-C factors by hypnotizability level and individual VSABTH-C item factor loadings are provided.

Metro orange line BRT project evaluation.

DOT National Transportation Integrated Search

2011-10-01

In partnership with the Los Angeles County Metropolitan Transportation Authority (Metro) and the Federal Transit Administration (FTA), the National Bus Rapid Transit Institute (NBRTI) conducted an evaluation of the Metro Orange Line BRT service, whic...

Low temperature solution process-based defect-induced orange-red light emitting diode

PubMed Central

Biswas, Pranab; Baek, Sung-Doo; Hoon Lee, Sang; Park, Ji-Hyeon; Jeong Lee, Su; Il Lee, Tae; Myoung, Jae-Min

2015-01-01

We report low-temperature solution-processed p-CuO nanorods (NRs)/n-ZnO NRs heterojunction light emitting diode (LED), exploiting the native point defects of ZnO NRs. ZnO NRs were synthesized at 90 °C by using hydrothermal method while CuO NRs were synthesized at 100 °C by using microwave reaction system. The electrical properties of newly synthesized CuO NRs revealed a promising p-type nature with a hole concentration of 9.64 × 1018 cm−3. The current-voltage characteristic of the heterojunction showed a significantly high rectification ratio of 105 at 4 V with a stable current flow. A broad orange-red emission was obtained from the forward biased LED with a major peak at 610 nm which was attributed to the electron transition from interstitial zinc to interstitial oxygen point defects in ZnO. A minor shoulder peak was also observed at 710 nm, corresponding to red emission which was ascribed to the transition from conduction band of ZnO to oxygen vacancies in ZnO lattice. This study demonstrates a significant progress toward oxide materials based, defect-induced light emitting device with low-cost, low-temperature methods. PMID:26648420

Effects of essential oil exposure on salivary estrogen concentration in perimenopausal women.

PubMed

Shinohara, Kazuyuki; Doi, Hirokazu; Kumagai, Chizu; Sawano, Erika; Tarumi, Wataru

2017-01-01

The menopausal transition is the time from the onset of menstrual changes until one year after the final menstrual period. During this phase, perimenopausal women experience a variety of health-related symptoms, which seemingly derive from declining level of estrogen secretion. It has long been recognized that some essential oils have the efficacy of alleviating menopausal symptoms. On the basis of this, it is possible that these essential oils have the potency to facilitate estrogen secretion in women. The present study investigated this possibility by examining if the olfactory exposure to the essential oil increase salivary estrogen concentration. We tested the effect of ten essential oils; clary sage, frankincense, geranium, lavender, jasmine absolute, neroli, rose otto, ylang ylang, orange and roman chamomile, which are thought to relieve perimenopasal symptoms. The results have shown increase of salivary estrogen concentration induced by exposure to geranium and rose otto compared to control odor. Together with the previous studies, the present study may give support to the notion that olfactory exposure to some essential oils can influence salivary concentration of estrogen.

Characterization and luminescence properties of Sr3Gd): Sm3+ orange-red phosphor

NASA Astrophysics Data System (ADS)

Yang, Zaifa; Xu, Denghui; Sun, Jiayue; Sun, Yumei; Du, Haiyan

2015-10-01

Reddish-orange emitting phosphors, Sr3Gd): Sm3+, were successfully synthesized by a conventional solid-state reaction. The crystal structure of the phosphors was characterized by x-ray diffraction. The excitation spectra and emission spectra were utilized to characterize the luminescence properties of the as-prepared phosphors. The results show that the phosphor consisted of some sharp emission peaks of Sm3+ ions centered at 564, 600, 647, and 707 nm, respectively. The critical distance of Sr3Gd0.93): 0.07Sm3+ was calculated to be 19.18 Å and the lifetime value of the sample was 1.63 ms. The band gap of Sr3Gd) was estimated to be about 2.74 eV from the diffuse reflection spectrum. The optimum doping concentration is 7 mol. % and the quenching occurs via dipole-dipole interaction according to Dexter's theory. The Commission Internationale de L'Eclairage value of Sr3Gd): Sm3+ phosphors presented that it has high color purity. These results indicated that the Sr3Gd): Sm3+ may be a promising reddish-orange emitting phosphor for cost-effective near ultraviolet white light-emitting diodes.

Lunar volcanism - Age of the glass in the Apollo 17 orange soil.

NASA Technical Reports Server (NTRS)

Husain, L.; Schaeffer, O. A.

1973-01-01

Glasses on the moon can form either by impact or by volcanic processes. An age determination indicates that the glass of the orange soil formed close in time to the volcanic activity in the Sea of Serenity about 3,750 million years ago. The orange soil was certainly not formed by a recent fumarole. The young exposure age, about 32 million years, found for the glass of the orange soil is in agreement with its fresh appearance on the lunar surface.

Effect of orange rust on sugarcane breeding program at canal Point

USDA-ARS?s Scientific Manuscript database

Orange rust of sugarcane (Saccharum spp. hybrids), caused by Puccinia kuehnii (W. Krüger) E.J. Butler, appeared in the Western Hemisphere ten years ago. Orange rust substantially reduces yields in susceptible sugarcane genotypes. Majority of the commercial cultivars were susceptible at the time of o...

76 FR 71108 - Applications of Orange Air, LLC for Certificate Authority

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-16

... DEPARTMENT OF TRANSPORTATION Office of the Secretary Applications of Orange Air, LLC for Certificate Authority AGENCY: Department of Transportation. ACTION: Notice of Order to Show Cause (Order 2011... directing all interested persons to show cause why it should not issue orders finding Orange Air, LLC, fit...

Seville orange juice-felodipine interaction: comparison with dilute grapefruit juice and involvement of furocoumarins.

PubMed

Malhotra, S; Bailey, D G; Paine, M F; Watkins, P B

2001-01-01

Our objective was to determine whether Seville orange juice produces a grapefruit juice-like interaction with felodipine and whether bergamottin, 6',7'-dihydroxybergamottin, or other furocoumarins are involved. In a randomized three-way crossover design, 10 volunteers received a felodipine 10-mg extended-release tablet with 240 mL of Seville orange juice, dilute grapefruit juice (that contained equivalent total molar concentrations of bergamottin plus 6',7'-dihydroxybergamottin), or common orange juice (negative control). The pharmacokinetics of felodipine and its dehydrofelodipine metabolite were determined. Juice concentrations of furocoumarins were measured. CYP3A4 inhibitory activity of newly identified furocoumarins was assessed. The felodipine area under the plasma concentration-time curve was increased by 76% and 93% after Seville orange juice and grapefruit juice ingestion, respectively, compared with common orange juice. The effects of Seville orange juice and grapefruit juice were similar in that the felodipine maximum concentration was augmented while the terminal elimination half-life was unchanged and the dehydrofelodipine area under the plasma concentration time-curve was increased, but the dehydrofelodipine-felodipine area under the plasma concentration-time curve ratio was reduced. Bergamottin and 6',7'-dihydroxybergamottin concentrations were 5 and 36 micromol/L, respectively, in Seville orange juice and were 16 and 23 micromol/L, respectively, in dilute grapefruit juice. A newly identified furocoumarin, bergapten, was detected only in Seville orange juice (31 micromol/L), and it was found to be a mechanism-based inhibitor of recombinant CYP3A4. Relative to the control, 6',7'-dihydroxybergamottin (10 micromol/L) inhibited CYP3A4 activity in cultured intestinal epithelial cells by 93%, whereas bergapten (10 micromol/L) inhibited the activity by only 34%. Seville orange juice and grapefruit juice interact with felodipine by a common mechanism, which

Repellent Activity of Botanical Oils against Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Liviidae)

PubMed Central

Kuhns, Emily H.; Martini, Xavier; Hoyte, Angel; Stelinski, Lukasz L.

2016-01-01

The Asian citrus psyllid, Diaphorina citri Kuwayama, is the insect vector of the pathogen causing huanglongbing. We selected three botanical oils to evaluate behavioral activity against D. citri. In laboratory olfactometer assays, fir oil was repellent to D. citri females, while litsea and citronella oils elicited no response from D. citri females. In choice settling experiments, D. citri settled almost completely on control plants rather than on plants treated with fir oil at a 9.5 mg/day release rate. Therefore, we conducted field trials to determine if fir oil reduced D. citri densities in citrus groves. We found no repellency of D. citri from sweet orange resets that were treated with fir oil dispensers releasing 10.4 g/day/tree as compared with control plots. However, we found a two-week decrease in populations of D. citri as compared with controls when the deployment rate of these dispensers was doubled. Our results suggest that treatment of citrus with fir oil may have limited activity as a stand-alone management tool for D. citri and would require integration with other management practices. PMID:27429006

Subspecies identification of captive Orang Utan in Melaka based on D-loop mitochondria DNA

NASA Astrophysics Data System (ADS)

Kamaluddin, Siti Norsyuhada; Yaakop, Salmah; Idris, Wan Mohd Razi; Rovie-Ryan, Jeffrine Japning; Md-Zain, Badrul Munir

2018-04-01

Mitochondrial DNA of Bornean Orang Utan populations suggests that there are three different subspecies (Pongo pygmaeus pygmaeus; Sarawak & Northwest Kalimantan, P. p. wurmbii; Southern West Kalimantan and Central Kalimantan, P. p. morio; East Kalimantan and Sabah). The subspecies of Orang Utans in captivity are difficult to determine through morphological observation. Thus, misidentification by ranger or zoo staffs leads to unwanted consequences especially towards conservation efforts of Orang Utan. The main objective of this study was to identify the subspecies and the geographic origin of 10 Orang Utans in Zoo Melaka and A' Famosa by using partial mitochondrial D-loop gene sequences. DNA of all individuals was extracted from FTA Card. Data analyses were performed using Maximum Parsimony, MP and Neighbor Joining, NJ. Molecular phylogeny analysis revealed that all the samples likely belong to one species of Sumatran Orang Utan (P. abelii) and three different subspecies of Bornean Orang Utans (P. p. pygmaeus, P. p. morio, and P. p. wurmbii). The results obtained in this study indirectly help the management of zoos in term of conservation and visitor's education.

Nuclear magnetic resonance characterization of metabolite disorder in orange trees caused by citrus sudden death disease.

PubMed

Prestes, Rosilene A; Colnago, Luiz A; Forato, Lucimara A; Carrilho, Emanuel; Bassanezi, Renato B; Wulff, Nelson A

2009-01-01

Citrus sudden death (CSD) is a new disease of sweet orange and mandarin trees grafted on Rangpur lime and Citrus volkameriana rootstocks. It was first seen in Brazil in 1999, and has since been detected in more than four million trees. The CSD causal agent is unknown and the current hypothesis involves a virus similar to Citrus tristeza virus or a new virus named Citrus sudden death-associated virus. CSD symptoms include generalized foliar discoloration, defoliation and root death, and, in most cases, it can cause tree death. One of the unique characteristics of CSD disease is the presence of a yellow stain in the rootstock bark near the bud union. This region also undergoes profound anatomical changes. In this study, we analyse the metabolic disorder caused by CSD in the bark of sweet orange grafted on Rangpur lime by nuclear magnetic resonance (NMR) spectroscopy and imaging. The imaging results show the presence of a large amount of non-functional phloem in the rootstock bark of affected plants. The spectroscopic analysis shows a high content of triacylglyceride and sucrose, which may be related to phloem blockage close to the bud union. We also propose that, without knowing the causal CSD agent, the determination of oil content in rootstock bark by low-resolution NMR can be used as a complementary method for CSD diagnosis, screening about 300 samples per hour.

Engineering d-limonene synthase down-regulation in orange fruit induces resistance against the fungus Phyllosticta citricarpa through enhanced accumulation of monoterpene alcohols and activation of defence.

PubMed

Rodríguez, Ana; Kava, Vanessa; Latorre-García, Lorena; da Silva, Geraldo J; G Pereira, Rosana; Glienke, Chirlei; Ferreira-Maba, Lisandra S; Vicent, Antonio; Shimada, Takehiko; Peña, Leandro

2018-03-24

Terpene volatiles play an important role in the interactions between specialized pathogens and fruits. Citrus black spot (CBS), caused by the fungus Phyllosticta citricarpa, is associated with crop losses in different citrus-growing areas worldwide. The pathogen may infect the fruit for 20-24 weeks after petal fall, but the typical hard spot symptoms appear when the fruit have almost reached maturity, caused by fungal colonization and the induction of cell lysis around essential oil cavities. d-Limonene represents approximately 95% of the total oil gland content in mature orange fruit. Herein, we investigated whether orange fruit with reduced d-limonene content in peel oil glands via an antisense (AS) approach may affect fruit interaction with P. citricarpa relative to empty vector (EV) controls. AS fruit showed enhanced resistance to the fungus relative to EV fruit. Because of the reduced d-limonene content, an over-accumulation of linalool and other monoterpene alcohols was found in AS relative to EV fruit. A global gene expression analysis at 2 h and 8 days after inoculation with P. citricarpa revealed the activation of defence responses in AS fruit via the up-regulation of different pathogenesis-related (PR) protein genes, probably as a result of enhanced constitutive accumulation of linalool and other alcohols. When assayed in vitro and in vivo, monoterpene alcohols at the concentrations present in AS fruit showed strong antifungal activity. We show here that terpene engineering in fruit peels could be a promising method for the development of new strategies to obtain resistance to fruit diseases. © 2018 BSPP and John Wiley & Sons Ltd.

L-MEB Model Calibration Over the Valencia Anchor Station Area

NASA Astrophysics Data System (ADS)

Lopez-Baeza, E.

2009-04-01

In the framework of ESA's SMOS (Soil Moisture and Ocean Salinity) Mission, several studies are being carried out over different types of land surfaces to study their microwave L-band emission (1.4 GHz). These studies are being integrated in the SMOS emission model (L-MEB, L-band Microwave Emission of the Biosphere, Wigneron et al. 2007), which is the core of the SMOS algorithm for the retrieval of land surface parameters from SMOS data. To contribute to Cal/Val activities at the Valencia Anchor Station (VAS) area (Caudete de las Fuentes, Valencia, Spain), one of the primary validation areas for SMOS land data and products (ESA SMOS Cal/Val AO, Project ID 3252, Lopez-Baeza et al., 2005), a number of experiments have been carried out to study the vegetation influence over the L-band emission proceeding from the soil surface. In the VAS area, a reduced number of homogeneous units have been defined according to the type and use of the soil, mainly, shrubs, vineyards, orchards (almond-and olive-trees) and Mediterranean pine forests. In order to implement the SMOS algorithm over this reference area, it is necessary to characterize and calibrate the L-MEB model for the different cover types. This work is significantly contributing to the definition of the VAS site as a validation area for SMOS land products of the size of a SMOS pixel (SMOS reference pixel). Shrubs and vineyards are the two most significant vegetation types which cover a large percentage of the area and for which very little information at L-band is available in the literature. These two types of vegetation covers have been studied in two separate dedicated experiments under the common name of MELBEX (Mediterranean Ecosystem L-Band characterisation EXperiment). The first one (MELBEX-I) took place over a shrub area characterised by a significant proportion of bare soil with superficial stones. The second one (MELBEX-II) was carried out from March to December 2007 over a large vineyard area. During the time

Acidification of apple and orange hosts by Penicillium digitatum and Penicillium expansum.

PubMed

Vilanova, L; Viñas, I; Torres, R; Usall, J; Buron-Moles, G; Teixidó, N

2014-05-16

New information about virulence mechanisms of Penicillium digitatum and Penicillium expansum could be an important avenue to control fungal diseases. In this study, the ability of P. digitatum and P. expansum to enhance their virulence by locally modulating the pH of oranges and apples was evaluated. For each host, pH changes with a compatible pathogen and a non-host pathogen were recorded, and the levels of different organic acids were evaluated to establish possible relationships with host pH modifications. Moreover, fruits were harvested at three maturity stages to determine whether fruit maturity could affect the pathogens' virulence. The pH of oranges and apples decreased when the compatible pathogens (P. digitatum and P. expansum, respectively) decayed the fruit. The main organic acid detected in P. digitatum-decayed oranges was galacturonic acid produced as a consequence of host maceration in the rot development process. However, the obtained results showed that this acid was not responsible for the pH decrease in decayed orange tissue. The mixture of malic and citric acids could at least contribute to the acidification of P. digitatum-decayed oranges. The pH decrease in P. expansum decayed apples is related to the accumulation of gluconic and fumaric acids. The pH of oranges and apples was not affected when the non-host pathogen was not able to macerate the tissues. However, different organic acid contents were detected in comparison to healthy tissues. The main organic acids detected in P. expansum-oranges were oxalic and gluconic and in P. digitatum-apples were citric, gluconic and galacturonic. Further research is needed to identify the pathogenicity factors of both fungi because the contribution of organic acids has profound implications. Copyright © 2014 Elsevier B.V. All rights reserved.

Improvement of biogas production from orange peel waste by leaching of limonene.

PubMed

Wikandari, Rachma; Nguyen, Huong; Millati, Ria; Niklasson, Claes; Taherzadeh, Mohammad J

2015-01-01

Limonene is present in orange peel wastes and is known as an antimicrobial agent, which impedes biogas production when digesting the peels. In this work, pretreatment of the peels to remove limonene under mild condition was proposed by leaching of limonene using hexane as solvent. The pretreatments were carried out with homogenized or chopped orange peel at 20-40°C with orange peel waste and hexane ratio (w/v) ranging from 1 : 2 to 1 : 12 for 10 to 300 min. The pretreated peels were then digested in batch reactors for 33 days. The highest biogas production was achieved by treating chopped orange peel waste and hexane ratio of 12 : 1 at 20°C for 10 min corresponding to more than threefold increase of biogas production from 0.061 to 0.217 m(3) methane/kg VS. The solvent recovery was 90% using vacuum filtration and needs further separation using evaporation. The hexane residue in the peel had a negative impact on biogas production as shown by 28.6% reduction of methane and lower methane production of pretreated orange peel waste in semicontinuous digestion system compared to that of untreated peel.

Prediction and functional analysis of the sweet orange protein-protein interaction network.

PubMed

Ding, Yu-Duan; Chang, Ji-Wei; Guo, Jing; Chen, Dijun; Li, Sen; Xu, Qiang; Deng, Xiu-Xin; Cheng, Yun-Jiang; Chen, Ling-Ling

2014-08-05

Sweet orange (Citrus sinensis) is one of the most important fruits world-wide. Because it is a woody plant with a long growth cycle, genetic studies of sweet orange are lagging behind those of other species. In this analysis, we employed ortholog identification and domain combination methods to predict the protein-protein interaction (PPI) network for sweet orange. The K-nearest neighbors (KNN) classification method was used to verify and filter the network. The final predicted PPI network, CitrusNet, contained 8,195 proteins with 124,491 interactions. The quality of CitrusNet was evaluated using gene ontology (GO) and Mapman annotations, which confirmed the reliability of the network. In addition, we calculated the expression difference of interacting genes (EDI) in CitrusNet using RNA-seq data from four sweet orange tissues, and also analyzed the EDI distribution and variation in different sub-networks. Gene expression in CitrusNet has significant modular features. Target of rapamycin (TOR) protein served as the central node of the hormone-signaling sub-network. All evidence supported the idea that TOR can integrate various hormone signals and affect plant growth. CitrusNet provides valuable resources for the study of biological functions in sweet orange.

Orange interventions for symptoms associated with dimethyl sulfoxide during stem cell reinfusions: a feasibility study.

PubMed

Potter, Pamela; Eisenberg, Seth; Cain, Kevin C; Berry, Donna L

2011-01-01

For over 2 decades, oncology nurses at a regional comprehensive cancer center offered sliced oranges to patients during the reinfusion of autologous hematopoietic progenitor cells (HPCs) to relieve symptoms associated with the preservative dimethyl sulfoxide (DMSO). This randomized pilot study examined feasibility and efficacy of sliced orange intervention (OI), orange aromatherapy intervention (OAI), or deep breathing (control) to address unpleasant adverse effects during HPC infusion. Orange intervention sniffed or tasted a quartered orange, OAI sniffed orange aromatherapy, and control took deep breaths. Perceived "symptom intensity" for tickle/cough urge, nausea, retching, and perceived "relief" were measured on 0- to 10-point numerical scales. Sixty of 72 eligible patients consented to participate and were randomized to OI (n = 19), OAI (n = 23), or control (n = 18). Study personnel successfully administered study procedures. Over the course of 2 bags of cells infused, the OI group reported significantly greater relief with the intervention (P = .032). Among participants less than 90 kg, OI group reported significantly lower symptom intensity (P = .012). Results suggest a feasible protocol and potential efficacy of sliced oranges for treating symptoms associated with DMSO-preserved stem cells. Study procedures provide a tested protocol for future studies. Follow-up study is warranted to confirm these findings and evaluate other treatment options. Oranges offer a simple, noninvasive intervention for relieving symptoms associated with DMSO preservative during autologous HPC infusion.

Improvement of stability and carotenoids fraction of virgin olive oils by addition of microalgae Scenedesmus almeriensis extracts.

PubMed

Limón, Piedad; Malheiro, Ricardo; Casal, Susana; Acién-Fernández, F Gabriel; Fernández-Sevilla, José M; Rodrigues, Nuno; Cruz, Rebeca; Bermejo, Ruperto; Pereira, José Alberto

2015-05-15

Humans are not capable of synthesizing carotenoids de novo and thus, their presence in human tissues is entirely of dietary origin. Consumption of essential carotenoids is reduced due to the lower intake of fruits and vegetables. Microalgae are a good source of carotenoids that can be exploited. In the present work, carotenoids rich extracts from Scenedesmus almeriensis were added to extra-virgin olive oils at different concentrations (0.1 and 0.21 mg/mL) in order to enhance the consumption of these bioactives. Extracts brought changes in olive oils color, turning them orange-reddish. Quality of olive oils was improved, since peroxidation was inhibited. Olive oils fatty acids and tocopherols were not affected. β-carotene and lutein contents increase considerably, as well as oxidative stability, improving olive oils shelf-life and nutritional value. Inclusion of S. almeriensis extracts is a good strategy to improve and enhance the consumption of carotenoids, since olive oil consumption is increasing. Copyright © 2014 Elsevier Ltd. All rights reserved.

77 FR 3326 - Cancellation of Environmental Impact Statement in Orange County, NC

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-23

... DEPARTMENT OF TRANSPORTATION Federal Highway Administration Cancellation of Environmental Impact Statement in Orange County, NC AGENCY: Federal Highway Administration (FHWA), North Carolina Department of... US 70 Business and US 70 Bypass in Orange County, North Carolina. The project is now cancelled...

Orange juice modulates proinflammatory cytokines after high-fat saturated meal consumption.

PubMed

Rocha, Daniela M U P; Lopes, Lílian L; da Silva, Alessandra; Oliveira, Leandro L; Bressan, Josefina; Hermsdorff, Helen Hermana M

2017-12-13

We aimed to evaluate the postprandial secretion of inflammatory markers induced by SFA or MUFA high-fat meal consumption and whether orange juice intake could modulate this induction. This study included 55 healthy women (aged 20 to 40 years): 33 participants received an SFA high-fat meal (≈1000 kcal, 37.6% of energy intake (E) from SFA) and 22 participants received an MUFA high-fat meal (≈1000 kcal, 56.3% E from MUFA). Both interventions were accompanied by 500 ml of orange juice (test) or water (control). The plasma concentrations of inflammatory cytokines (IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ, and TNF-α) and CRP were determined before (fasting) and 2, 3 and 5 hours after the test meal intake. The SFA high-fat meal induced a significant increase in AUC values (for TNF-α, IL-12, IL-10, IL-6 and IL-2 adjusted for baseline concentrations) in comparison with MUFA high-fat meal intervention. The results were independent of the drink which accompanied the meal (water or orange juice). Both IL-4 and IL-17A AUC values were significantly increased after an SFA high-fat meal intake, accompanied by water, but not by orange juice. In addition, these values were higher in relation to MUFA high-fat meal interventions. Also, IL-17A significantly increased at 3 h after an SFA high-fat meal intake accompanied by water, but not by orange juice. Overall, our conclusions indicate an anti-inflammatory effect of MUFA compared to SFA high-fat meal intake, while orange juice intake was able to mitigate the subclinical increase of postprandial inflammation, induced by SFA high-fat meal consumption, for a particular biomarker (IL-17A).

Orange-Pigmented Sputum as a Manifestation of Smoke Grenade Inhalation Injury.

PubMed

Chatzivasiloglou, Fotini; Katsenos, Stamatis; Psara, Anthoula; Tsintiris, Konstantinos

2016-01-01

A 34-year-old man presented with scanty hemoptysis, orange-colored expectoration, and mild dyspnea. He was in an enclosed building taking part in a military training exercise inhaling an orange-colored smoke from a smoke grenade ignition. His symptoms developed immediately after the initial exposure but he sought medical assistance 20 hours later because of their persistence. Fiberoptic bronchoscopy was performed revealing diffuse inflammatory tracheobronchial tree with streaky orange-pigmented secretions in the trachea and both main-stem bronchi. Acute tracheobronchitis was diagnosed and the patient was treated with nebulized bronchodilators and intravenous corticosteroids showing complete recovery. To our knowledge, this is the first well-documented report of inhalation injury induced by a smoke bomb explosion including potassium chlorate oxidizer and Sudan I and presenting with orange-pigmented sputum production. Smoke inhalation injury is associated with significant morbidity and mortality. The heterogeneity of the smoke and the large variety of the resulting symptoms may be the reason why a definition, specific diagnostic criteria, and therapeutic guidelines are still lacking.

Toxic Epidemics: Agent Orange Sickness in Vietnam and the United States.

PubMed

Uesugi, Tak

2016-01-01

Social scientists studying toxic epidemics have often endeavored to shed light on the differences between scientists' and nonscientists' epistemic perspectives. Yet, little attention has been paid to the processes through which a toxic epidemic emerges as a phenomenon. A Luoi Valley of Central Vietnam was extensively sprayed with chemical defoliants (including Agent Orange) during the Vietnam War. The latent toxic effects of these chemicals, however, went largely unnoticed until the late 1990s. By juxtaposing the history through which the notion of "Agent Orange Sickness" emerged in the United States with an ethnographic study of A Luoi, I explore the notion of poison under which Agent Orange became recognizable as a poison.

Incorporation of citrus essential oils into bacterial cellulose-based edible films and assessment of their physical properties

NASA Astrophysics Data System (ADS)

Indrarti, L.; Indriyati

2017-03-01

The use of edible films in food protection and preservation has recently gained more interest since they offer several advantages over synthetic packaging materials. Biocellulose (BC) offers great opportunity as edible film due to their unique physical and mechanical properties. In this study, biocellulose films were prepared by solution casting with addition of 30% carboxymethyl cellulose (CMC) and 30% glycerol as the homogenizer and plasticizer, respectively. Furthermore, various citrus essential oils (EOs) including lemon, lime, and sweet orange were added at 50% w/w of BC dried weight. The solutions were then cast on the tray and allowed to dry in the air convection oven at 40°C overnight. The films were characterized for water solubility, tensile strength (TS), elongation at break (EB), water vapour transmission rate (WVTR), and color. Those characteristics may influence consumer acceptability of the packaged products. Results revealed that addition of lemon and sweet orange EOs into BC-based edible film decreased water solubility and TS, but improved EB, as these oils acted as plasticizers in the film. However, different trend was observed for BC-based film incorporated with lime oil, which had higher solubility and TS, but lower EB and WVTR compared with that of control film. Addition of citrus EOs into BC-based films did not have much effect on color properties as stated in L*, a*, and b* values.

A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo

PubMed Central

Chu, Jun; Oh, Young-Hee; Sens, Alex; Ataie, Niloufar; Dana, Hod; Macklin, John J.; Laviv, Tal; Welf, Erik S.; Dean, Kevin M.; Zhang, Feijie; Kim, Benjamin B.; Tang, Clement Tran; Hu, Michelle; Baird, Michelle A.; Davidson, Michael W.; Kay, Mark A.; Fiolka, Reto; Yasuda, Ryohei; Kim, Douglas S.; Ng, Ho-Leung; Lin, Michael Z.

2016-01-01

Orange-red fluorescent proteins (FPs) are widely used in biomedical research for multiplexed epifluorescence microscopy with GFP-based probes, but their different excitation requirements make multiplexing with new advanced microscopy methods difficult. Separately, orange-red FPs are useful for deep-tissue imaging in mammals due to the relative tissue transmissibility of orange-red light, but their dependence on illumination limits their sensitivity as reporters in deep tissues. Here we describe CyOFP1, a bright engineered orange-red FP that is excitable by cyan light. We show that CyOFP1 enables single-excitation multiplexed imaging with GFP-based probes in single-photon and two-photon microscopy, including time-lapse imaging in light-sheet systems. CyOFP1 also serves as an efficient acceptor for resonance energy transfer from the highly catalytic blue-emitting luciferase NanoLuc. An optimized fusion of CyOFP1 and NanoLuc, called Antares, functions as a highly sensitive bioluminescent reporter in vivo, producing substantially brighter signals from deep tissues than firefly luciferase and other bioluminescent proteins. PMID:27240196

Sexual Competitiveness of Anastrepha ludens (Diptera: Tephritidae) Males Exposed to Citrus aurantium and Citrus paradisi Essential Oils.

PubMed

Morató, Santiago; Shelly, Todd; Rull, Juan; Aluja, Martin

2015-04-01

Males of the Mediterranean fruit fly (Ceratitis capitata (Wiedemann)) display increased mating competitiveness following exposure to the odor of certain host and nonhost plants, and this phenomenon has been used in the sterile insect technique to boost the mating success of released, sterile males. Here, we aimed to establish whether males of the Mexican fruit fly (Anastrepha ludens (Loew)) gain a mating advantage when exposed to the aroma of two preferred hosts, grapefruit (Citrus paradisi Macfadyen) and bitter orange (Citrus aurantium L.). Under seminatural conditions, we observed that, in trials using wildish males (from a young laboratory colony started with wild flies) exclusively, exposure to the aroma of bitter orange had no effect on male mating success but exposure to the odor grapefruit oil increased male mating success significantly. In a separate test involving both exposed and nonexposed wildish and mass-reared, sterile males, although wildish males were clearly more competitive than sterile males, exposure to grapefruit oil had no detectable effect on either male type. Exposure to oils had no effect on copulation duration in any of the experiments. We discuss the possibility that the positive effect of grapefruit essential oils on wildish male competitiveness may have been linked to exposure of females to grapefruit as a larval food, which may have imprinted them with grapefruit odors during pupal eclosion and biased their response as adults to odors of their maternal host. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Effects of tooth whitening and orange juice on surface properties of dental enamel.

PubMed

Ren, Yan-Fang; Amin, Azadeh; Malmstrom, Hans

2009-06-01

To study the effects of 6% H2O2 activated with LED light on surface enamel as compared to orange juice challenges in vitro. A total of 40 human enamel discs were incubated in saliva overnight to allow pellicle formation and then divided into three groups: 15 for whitening treatments, 15 for orange juice immersions and 10 for normal saline controls. Baseline microhardness was measured with a microhardness Knoop indenter (50g, 10s) and surface topography was evaluated with a focus-variation 3D scanning microscopy. Enamel discs were treated with H2O2 or orange juice for 20 min each cycle for five cycles to simulate daily treatment with the products for 5 days. The discs were stored in saliva between treatment cycles. Microhardness and surface topography were evaluated again after treatments. Changes in microhardness and in surface area roughness (Sa), mean maximum peak-to-valley distance (Sz) and the developed surface area ratio (Sdr) were compared before and after treatments (t-test) and among groups (ANOVA). Enamel surface hardness decreased by 84% after orange juice immersion but no statistically significant changes were observed in the whitening and control groups. Surface topography changed significantly only in the orange juice group as shown by increased Sa (1.2 microm vs. 2.0 microm), Sz (7.7 microm vs. 10.2 microm) and Sdr (2.8% vs. 6.0%). No such changes were observed in the whitening and control groups. In comparison to orange juice challenges, the effects of 6% H2O2 on surface enamel are insignificant. Orange juice erosion markedly decreased hardness and increased roughness of enamel.

Agent Orange exposure and attributed health effects in Vietnam veterans.

PubMed

Young, Alvin L; Cecil, Paul F

2011-07-01

Serum dioxin studies of Vietnam (VN) veterans, military historical records of tactical herbicide use in Vietnam, and the compelling evidence of the photodegradation of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and other aspects of environmental fate and low bioavailability of TCDD are consistent with few, if any, ground troop veterans being exposed to Agent Orange. That conclusion, however, is contrary to the presumption by the Department of Veterans Affairs (DVA) that military service in Vietnam anytime from January 9, 1962 to May 7, 1975 is a proxy for exposure to Agent Orange. The DVA assumption is inconsistent with the scientific principles governing determinations of disease causation. The DVA has nonetheless awarded Agent Orange-related benefits and compensation to an increasing number of VN veterans based on the presumption of exposure and the published findings of the Institute of Medicine that there is sufficient evidence of a "statistical association" (a less stringent standard than "causal relationship") between exposure to tactical herbicides or TCDD and 15 different human diseases. A fairer and more valid approach for VN veterans would have been to enact a program of "Vietnam experience" benefits for those seriously ill, rather than benefits based on the dubious premise of injuries caused by Agent Orange.

Improvement of Biogas Production from Orange Peel Waste by Leaching of Limonene

PubMed Central

Wikandari, Rachma; Nguyen, Huong; Millati, Ria; Niklasson, Claes; Taherzadeh, Mohammad J.

2015-01-01

Limonene is present in orange peel wastes and is known as an antimicrobial agent, which impedes biogas production when digesting the peels. In this work, pretreatment of the peels to remove limonene under mild condition was proposed by leaching of limonene using hexane as solvent. The pretreatments were carried out with homogenized or chopped orange peel at 20–40°C with orange peel waste and hexane ratio (w/v) ranging from 1 : 2 to 1 : 12 for 10 to 300 min. The pretreated peels were then digested in batch reactors for 33 days. The highest biogas production was achieved by treating chopped orange peel waste and hexane ratio of 12 : 1 at 20°C for 10 min corresponding to more than threefold increase of biogas production from 0.061 to 0.217 m3 methane/kg VS. The solvent recovery was 90% using vacuum filtration and needs further separation using evaporation. The hexane residue in the peel had a negative impact on biogas production as shown by 28.6% reduction of methane and lower methane production of pretreated orange peel waste in semicontinuous digestion system compared to that of untreated peel. PMID:25866787

[Orange Platform].

PubMed

Toba, Kenji

2017-07-01

The Organized Registration for the Assessment of dementia on Nationwide General consortium toward Effective treatment in Japan (ORANGE platform) is a recently established nationwide clinical registry for dementia. This platform consists of multiple registries of patients with dementia stratified by the following clinical stages: preclinical, mild cognitive impairment, early-stage, and advanced-stage dementia. Patients will be examined in a super-longitudinal fashion, and their lifestyle, social background, genetic risk factors, and required care process will be assessed. This project is also notable because the care registry includes information on the successful, comprehensive management of patients with dementia. Therefore, this multicenter prospective cohort study will contribute participants to all clinical trials for Alzheimer's disease as well as improve the understanding of individuals with dementia.

Comparison of Nutritional Status between Children with Autism Spectrum Disorder and Typically Developing Children in the Mediterranean Region (Valencia, Spain)

ERIC Educational Resources Information Center

Marí-Bauset, Salvador; Llopis-González, Agustín; Zazpe, Itziar; Marí-Sanchis, Amelia; Morales Suárez-Varela, Maria

2017-01-01

This case-control study investigated nutrient intake, healthy eating index with 10 items on foods and nutrients, on 3-day food diaries and anthropometric measurements in 105 children with autism spectrum disorder and 495 typically developing children (6-9 years) in Valencia (Spain). Children with autism spectrum disorder were at a higher risk for…

Glyphosate Control of Orange and Brown Rusts in Glyphosate-Sensitive Sugarcane Cultivars

USDA-ARS?s Scientific Manuscript database

Technical Abstract: Brown and orange rust diseases cause substantial yield reductions on sugarcane (Saccharum spp.) in Florida and other regions where sugarcane is grown. Brown rust caused by Puccinia melanocephala Syd. & P. Syd has been present in Florida since 1978 and orange rust caused by Pucci...

Hydrogeology and quality of ground water in Orange County, Florida

USGS Publications Warehouse

Adamski, James C.; German, Edward R.

2004-01-01

Ground water is the main source of water supply in central Florida and is critical for aquatic habitats and human consumption. To provide a better understanding for the conservation, development, and management of the water resources of Orange County, Florida, a study of the hydrogeologic framework, water budget, and ground-water quality characteristics was conducted from 1998 through 2002. The study also included extensive analyses of the surface-water resources, published as a separate report. An increase in population from about 264,000 in 1960 to 896,000 in 2000 and subsequent urban growth throughout this region has been accompanied by a substantial increase in water use. Total ground-water use in Orange County increased from about 82 million gallons per day in 1965 to about 287 million gallons per day in 2000. The hydrogeology of Orange County consists of three major hydrogeologic units: the surficial aquifer system, the intermediate confining unit, and the Floridan aquifer system. Data were compiled from 634 sites to construct hydrogeologic maps and sections of Orange County. Water-level elevations measured in 23 wells tapping the surficial aquifer system ranged from about 10.6 feet in eastern Orange County to 123.8 feet above NGVD 29 in northwestern Orange County from March 2000 through September 2001. Water levels also were measured in 14 wells tapping the Upper Floridan aquifer. Water levels fluctuate over time from seasonal and annual variations in rainfall; however, water levels in a number of wells tapping the Upper Floridan aquifer have declined over time. Withdrawal of ground water from the aquifers by pumping probably is causing the declines because the average annual precipitation rate has not changed substantially in central Florida since the 1930s, although yearly rates can vary. A generalized water budget was computed for Orange County from 1991 to 2000. Average rates for the 10-year period for the following budget components were computed based

Effect of fermentation and subsequent pasteurization processes on amino acids composition of orange juice.

PubMed

Cerrillo, I; Fernández-Pachón, M S; Collado-González, J; Escudero-López, B; Berná, G; Herrero-Martín, G; Martín, F; Ferreres, F; Gil-Izquierdo, A

2015-06-01

The fermentation of fruit produces significant changes in their nutritional composition. An orange beverage has been obtained from the controlled alcoholic fermentation and thermal pasteurization of orange juice. A study was performed to determine the influence of both processes on its amino acid profile. UHPLC-QqQ-MS/MS was used for the first time for analysis of orange juice samples. Out of 29 amino acids and derivatives identified, eight (ethanolamine, ornithine, phosphoethanolamine, α-amino-n-butyric acid, hydroxyproline, methylhistidine, citrulline, and cystathionine) have not previously been detected in orange juice. The amino acid profile of the orange juice was not modified by its processing, but total amino acid content of the juice (8194 mg/L) was significantly increased at 9 days of fermentation (13,324 mg/L). Although the pasteurization process produced partial amino acid degradation, the total amino acid content was higher in the final product (9265 mg/L) than in the original juice, enhancing its nutritional value.

Limonoid content of sour orange varieties

USDA-ARS?s Scientific Manuscript database

Modern Citrus cultivars are thought to have arisen from three parents- the (pummelo), the mandarin, and citron. Taxological and genetic data support that sweet and sour oranges share a common parentage. However, as their name suggests the organoleptic properties of the fruit from these two familie...

Agent Orange exposure and cancer incidence in Korean Vietnam veterans: a prospective cohort study.

PubMed

Yi, Sang-Wook; Ohrr, Heechoul

2014-12-01

During the Vietnam War, US and allied military sprayed approximately 77 million liters of tactical herbicides including Agent Orange, contaminated with 2,3,7,8-tetrachlorodibenzo-p-dioxin. To the authors' knowledge, few studies to date have examined the association between Agent Orange exposure and cancer incidence among Korean veterans who were exposed to Agent Orange during the Vietnam War. An Agent Orange exposure index, based on the proximity of the veteran's military unit to the area that was sprayed with Agent Orange, was developed using a geographic information system-based model. Cancer incidence was followed for 180,251 Vietnam veterans from 1992 through 2003. After adjustment for age and military rank, high exposure to Agent Orange was found to significantly increase the risk of all cancers combined (adjusted hazards ratio [aHR], 1.08). Risks for cancers of the mouth (aHR, 2.54), salivary glands (aHR, 6.96), stomach (aHR, 1.14), and small intestine (aHR, 2.30) were found to be significantly higher in the high-exposure group compared with the low-exposure group. Risks for cancers of all sites combined (aHR, 1.02) and for cancers of the salivary glands (aHR, 1.47), stomach (aHR, 1.03), small intestine (aHR, 1.24), and liver (aHR, 1.02) were elevated with a 1-unit increase in the exposure index. Exposure to Agent Orange several decades earlier may increase the risk of cancers in all sites combined, as well as several specific cancers, among Korean veterans of the Vietnam War, including some cancers that were not found to be clearly associated with exposure to Agent Orange in previous cohort studies primarily based on Western populations. © 2014 American Cancer Society.

Hydrosols of orange blossom (Citrus aurantium), and rose flower (Rosa damascena and Rosa centifolia) support the growth of a heterogeneous spoilage microbiota.

PubMed

Labadie, Cécile; Ginies, Christian; Guinebretiere, Marie-Hélène; Renard, Catherine M G C; Cerutti, Céline; Carlin, Frédéric

2015-10-01

Hydrosols are hydrodistillation products of aromatic plants. They contain less than 1g/L of dispersed essential oils giving organoleptic properties. Hydrosols are subjected to microbial proliferation. Reasons for spoilage have to be found in the nature of substrates supporting growth and of microbiological contaminants. The composition in essential oils and the microbiota of 22 hydrosol samples of Citrus aurantium L. ssp. amara L. (orange blossom), Rosa damascena Miller (rose D.), and Rosa centifolia L. (rose C.) flowers were analyzed to determine the factors responsible for decay. The median concentrations in essential oils were 677mg/L for orange blossom hydrosols, 205mg/L for rose D. hydrosols, and 116mg/L for rose C. hydrosols. The dry matter content of these hydrosols varied between 4.0mg/L and 702mg/L, and the carbohydrate content varied between 0.21mg/L and 0.38mg/L. These non-volatile compounds were likely carried over during distillation by a priming and foaming effect, and could be used as nutrients by microorganisms. A microbial proliferation at ambient temperature and also at 5°C has been observed in all studied hydrosols when stored in a non-sterile container. In contaminated hydrosols, maximal counts were about 7log 10 CFU/mL, while the French pharmacopeia recommends a maximal total bacterial count of 2log 10 CFU/mL. Neither yeast nor mold was detected. The isolated microbial population was composed of environmental Gram-negative bacteria, arranged in four major genera: Pseudomonas sp., Burkholderia cepacia complex, and presumably two new genera belonging to Acetobacteraceae and Rhodospirillaceae. Among those bacteria, Burkholderia vietnamiensis and Novosphingobium capsulatum were able to metabolize volatile compounds, such as geraniol to produce 6-methyl-5-hepten-2-one or geranic acid, or phenylethyl acetate to produce 2-phenylethanol. EO concentrations in hydrosols or cold storage are not sufficient to insure microbiological stability. Additional

Essential Oils for Complementary Treatment of Surgical Patients: State of the Art

PubMed Central

Stea, Susanna; Beraudi, Alina; De Pasquale, Dalila

2014-01-01

Aromatherapy is the controlled use of plant essences for therapeutic purposes. Its applications are numerous (i.e., wellbeing, labour, infections, dementia, and anxiety treatment) but often they have not been scientifically validated. The aim of the present study is to review the available literature to determine if there is evidence for effectiveness of aromatherapy in surgical patients to treat anxiety and insomnia, to control pain and nausea, and to dress wound. Efficacy studies of lavender or orange and peppermint essential oils, to treat anxiety and nausea, respectively, have shown positive results. For other aspects, such as pain control, essential oils therapy has shown uncertain results. Finally, there are encouraging data for the treatment of infections, especially for tea tree oil, although current results are still inconclusive. It should also be considered that although they are, allergic reactions and toxicity can occur after oral ingestion. Therefore, while rigorous studies are being carried out, it is important that the therapeutic use of essential oils be performed in compliance with clinical safety standards. PMID:24707312

Increased risks of cardiovascular diseases and insulin resistance among the Orang Asli in Peninsular Malaysia.

PubMed

Tuan Abdul Aziz, Tuan Azlin; Teh, Lay Kek; Md Idris, Muhd Hanis; Bannur, Zakaria; Ashari, Lydiatul Shima; Ismail, Adzrool Idzwan; Ahmad, Aminuddin; Isa, Kamarudzaman Md; Nor, Fadzilah Mohd; Rahman, Thuhairah Hasrah Abdul; Shaari, Syahrul Azlin Binti; Jan Mohamed, Hamid Jan; Mohamad, Nornazliya; Salleh, Mohd Zaki

2016-03-24

Despite the strategic development plan by the authorities for the Orang Asli, there are six subtribes of which their population numbers are small (less than 700). These minorities were not included in most of the health related studies published thus far. A comprehensive physiological and biomedical updates on these small subtribes in comparison to the larger subtribes and the urban Malay population is timely and important to help provide appropriate measures to prevent further reduction in the numbers of the Orang Asli. A total of 191 Orang Asli from different villages in Peninsular Malaysia and 115 healthy urban Malays were recruited. Medical examinations and biochemical analyses were conducted. Framingham risk scores were determined. Data was analyzed using IBM SPSS Statistics, Version 20.0. A higher percentage of the Orang Asli showed high insulin levels and hsCRP compared to the healthy Malays denoting possible risk of insulin resistance. High incidences of low HDL-c levels were observed in all the Orang Asli from the six subtribes but none was detected among the urban Malays. A higher percentage of inlanders (21.1% of the males and 4.2% of the females) were categorized to have high Framingham Risk Score. Orang Asli staying both in the inlands and peripheries are predisposed to cardiovascular diseases and insulin resistance diabetes mellitus. The perception of Orang Asli being healthier than the urban people no longer holds. We believed that this information is important to the relevant parties in strategizing a healthier community of the Orang Asli to avoid the vanishing of the vulnerable group(s).

Ratiometric analysis of Acridine Orange staining in the study of acidic organelles and autophagy.

PubMed

Thomé, Marcos P; Filippi-Chiela, Eduardo C; Villodre, Emilly S; Migliavaca, Celina B; Onzi, Giovana R; Felipe, Karina B; Lenz, Guido

2016-12-15

Acridine Orange is a cell-permeable green fluorophore that can be protonated and trapped in acidic vesicular organelles (AVOs). Its metachromatic shift to red fluorescence is concentration-dependent and, therefore, Acridine Orange fluoresces red in AVOs, such as autolysosomes. This makes Acridine Orange staining a quick, accessible and reliable method to assess the volume of AVOs, which increases upon autophagy induction. Here, we describe a ratiometric analysis of autophagy using Acridine Orange, considering the red-to-green fluorescence intensity ratio (R/GFIR) to quantify flow cytometry and fluorescence microscopy data of Acridine-Orange-stained cells. This method measured with accuracy the increase in autophagy induced by starvation or rapamycin, and the reduction in autophagy produced by bafilomycin A1 or the knockdown of Beclin1 or ATG7. Results obtained with Acridine Orange, considering R/GFIR, correlated with the conversion of the unlipidated form of LC3 (LC3-I) into the lipidated form (LC3-II), SQSTM1 degradation and GFP-LC3 puncta formation, thus validating this assay to be used as an initial and quantitative method for evaluating the late step of autophagy in individual cells, complementing other methods. © 2016. Published by The Company of Biologists Ltd.

Essential Oils of Plants as Biocides against Microorganisms Isolated from Cuban and Argentine Documentary Heritage.

PubMed

Borrego, Sofía; Valdés, Oderlaise; Vivar, Isbel; Lavin, Paola; Guiamet, Patricia; Battistoni, Patricia; Gómez de Saravia, Sandra; Borges, Pedro

2012-01-01

Natural products obtained from plants with biocidal activity represent an alternative and useful source in the control of biodeterioration of documentary heritage, without negative environmental and human impacts. In this work, we studied the antimicrobial activity of seven essential oils against microorganisms associated with the biodeterioration of documentary heritage. The essential oils were obtained by steam distillation. The antimicrobial activity was analyzed using the agar diffusion method against 4 strains of fungi and 6 bacterial strains isolated from repositories air and documents of the National Archive of the Republic of Cuba and the Historical Archive of the Museum of La Plata, Argentina. Anise and garlic oils showed the best antifungal activity at all concentrations studied, while oregano oil not only was effective against fungi tested but also prevented sporulation of them all. Orange sweet and laurel oils were ineffective against fungi. Clove, garlic, and oregano oils showed the highest antibacterial activity at 25% against Enterobacter agglomerans and Streptomyces sp., while only clove and oregano oils were effective against Bacillus sp. at all concentrations studied. This study has an important implication for the possible use of the natural products from plants in the control of biodeterioration of documentary heritage.

Detection of mandarin in orange juice by single-nucleotide polymorphism qPCR assay.

PubMed

Aldeguer, Miriam; López-Andreo, María; Gabaldón, José A; Puyet, Antonio

2014-02-15

A dual-probe real time PCR (qPCR) DNA-based analysis was devised for the identification of mandarin in orange juice. A single nucleotide polymorphism at the trnL-trnF intergenic region of the chloroplast chromosome was confirmed in nine orange (Citrus sinensis) and thirteen commercial varieties of mandarin, including Citrus reticulata and Citrus unshiu species and a mandarin × tangelo hybrid. Two short minor-groove binding fluorescent probes targeting the polymorphic sequence were used in the dual-probe qPCR, which allowed the detection of both species in single-tube reactions. The similarity of PCR efficiencies allowed a simple estimation of the ratio mandarin/orange in the juice samples, which correlated to the measured difference of threshold cycle values for both probes. The limit of detection of the assay was 5% of mandarin in orange juice, both when the juice was freshly prepared (not from concentrate) or reconstituted from concentrate, which would allow the detection of fraudulently added mandarin juice. The possible use of the dual-probe system for quantitative measurements was also tested on fruit juice mixtures. qPCR data obtained from samples containing equal amounts of mandarin and orange juice revealed that the mandarin target copy number was approximately 2.6-fold higher than in orange juice. The use of a matrix-adapted control as calibrator to compensate the resulting C(T) bias allowed accurate quantitative measurements to be obtained. Copyright © 2013 Elsevier Ltd. All rights reserved.

Red Orange: Experimental Models and Epidemiological Evidence of Its Benefits on Human Health

PubMed Central

Galvano, Fabio; Mistretta, Antonio; Marventano, Stefano; Nolfo, Francesca; Calabrese, Giorgio; Buscemi, Silvio; Drago, Filippo; Veronesi, Umberto; Scuderi, Alessandro

2013-01-01

In recent years, there has been increasing public interest in plant antioxidants, thanks to the potential anticarcinogenic and cardioprotective actions mediated by their biochemical properties. The red (or blood) orange (Citrus sinensis (L.) Osbeck) is a pigmented sweet orange variety typical of eastern Sicily (southern Italy), California, and Spain. In this paper, we discuss the main health-related properties of the red orange that include anticancer, anti-inflammatory, and cardiovascular protection activities. Moreover, the effects on health of its main constituents (namely, flavonoids, carotenoids, ascorbic acid, hydroxycinnamic acids, and anthocyanins) are described. The red orange juice demonstrates an important antioxidant activity by modulating many antioxidant enzyme systems that efficiently counteract the oxidative damage which may play an important role in the etiology of numerous diseases, such as atherosclerosis, diabetes, and cancer. The beneficial effects of this fruit may be mediated by the synergic effects of its compounds. Thus, the supply of natural antioxidant compounds through a balanced diet rich in red oranges might provide protection against oxidative damage under differing conditions and could be more effective than, the supplementation of an individual antioxidant. PMID:23738032

Orange County Outdoor School: Teacher's Guide.

ERIC Educational Resources Information Center

Orange County Dept. of Education, Santa Ana, CA.

Divided into six sections, the guide provides helpful information for the teacher to prepare students to attend the Orange County Outdoor School. Pre-camp responsibilities section provides pre-camp preparation checklists for the principal, teacher, parents, school nurse, and outdoor specialist; a checklist for morning departure; discipline policy…

Carotenoid accumulation in orange-pigmented Capsicum annuum fruit, regulated at multiple levels.

PubMed

Rodriguez-Uribe, Laura; Guzman, Ivette; Rajapakse, Wathsala; Richins, Richard D; O'Connell, Mary A

2012-01-01

The pericarp of Capsicum fruit is a rich dietary source of carotenoids. Accumulation of these compounds may be controlled, in part, by gene transcription of biosynthetic enzymes. The carotenoid composition in a number of orange-coloured C. annuum cultivars was determined using HPLC and compared with transcript abundances for four carotenogenic enzymes, Psy, LcyB, CrtZ-2, and Ccs determined by qRT-PCR. There were unique carotenoid profiles as well as distinct patterns of transcription of carotenogenic enzymes within the seven orange-coloured cultivars. In one cultivar, 'Fogo', carrying the mutant ccs-3 allele, transcripts were detected for this gene, but no CCS protein accumulated. The premature stop termination in ccs-3 prevented expression of the biosynthetic activity to synthesize the capsanthin and capsorubin forms of carotenoids. In two other orange-coloured cultivars, 'Orange Grande' and 'Oriole', both with wild-type versions of all four carotenogenic enzymes, no transcripts for Ccs were detected and no red pigments accumulated. Finally, in a third case, the orange-coloured cultivar, Canary, transcripts for all four of the wild-type carotenogenic enzymes were readily detected yet no CCS protein appeared to accumulate and no red carotenoids were synthesized. In the past, mutations in Psy and Ccs have been identified as the loci controlling colour in the fruit. Now there is evidence that a non-structural gene may control colour development in Capsicum.

76 FR 36080 - Foreign-Trade Zone 37-Orange County, NY; Application for Reorganization (Expansion of Service...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-21

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Docket 42-2011] Foreign-Trade Zone 37--Orange... of Orange, New York, grantee of FTZ 37, requesting authority to reorganize its zone to expand its... service area that includes Orange County. The applicant is requesting authority to expand the service area...

78 FR 12033 - Foreign-Trade Zone 37-Orange County, NY, Application for Reorganization (Expansion of Service...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-21

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [B-14-2013] Foreign-Trade Zone 37--Orange County... application has been submitted to the Foreign-Trade Zones (FTZ) Board (the Board) by the County of Orange... service area that includes Orange and Duchess Counties, New York. The applicant is now requesting...

A role for the deep orange and carnation eye color genes in lysosomal delivery in Drosophila.

PubMed

Sevrioukov, E A; He, J P; Moghrabi, N; Sunio, A; Krämer, H

1999-10-01

Deep orange and carnation are two of the classic eye color genes in Drosophila. Here, we demonstrate that Deep orange is part of a protein complex that localizes to endosomal compartments. A second component of this complex is Carnation, a homolog of Sec1p-like regulators of membrane fusion. Because complete loss of deep orange function is lethal, the role of this complex in intracellular trafficking was analyzed in deep orange mutant clones. Retinal cells devoid of deep orange function completely lacked pigmentation and exhibited exaggerated multivesicular structures. Furthermore, a defect in endocytic trafficking was visualized in developing photoreceptor cells. These results provide direct evidence that eye color mutations of the granule group also disrupt vesicular trafficking to lysosomes.

Influence of the protonation state on the binding mode of methyl orange with cucurbiturils

NASA Astrophysics Data System (ADS)

He, Suhang; Sun, Xuzhuo; Zhang, Haibo

2016-03-01

Binding modes of methyl orange (MO) with cucurbiturils (CBs) have been investigated by Single Crystal X-ray Diffraction and NMR Spectroscopy. Detailed study of intermolecular interactions was supported by the Hirshfeld surface analysis. Protonation state of the anionic part of methyl orange has greatly influenced the binding mode of the complex. Stabilized by hydrogen bonding at the portal, hydrophobic and dispersion interactions in the cavity, the protonated methyl orange was deeply inserted into the cavity. On the contrary, the anionic methyl orange has been pushed towards the outside of the cavity by the electrostatic repulsion between the azo group and the portal oxygen. A ;water bridge; was found in MO@CB8 linking both host and guest via hydrogen bonds.

21 CFR 146.135 - Orange juice.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Orange juice. 146.135 Section 146.135 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION CANNED FRUIT JUICES Requirements for Specific Standardized Canned Fruit Juices and Beverages § 146...

Trapping characteristic of halloysite lumen for methyl orange

NASA Astrophysics Data System (ADS)

Chen, Hao; Yan, Hua; Pei, Zhenzhao; Wu, Junyong; Li, Rongrong; Jin, Yanxian; Zhao, Jie

2015-08-01

The interaction of clay minerals and dyes is an area of great interest especially in the development of novel adsorbents. In this report, we demonstrated interaction of halloysite nanotubes (HNTs) and an anionic dye, methyl orange (MO), through a electrostatic attraction. Halloysite lumen has a trapping characteristic for methyl orange, which is mainly determined by the positively charged nature of the inner surface of HNTs. XRD results confirmed that intercalation of methyl orange into HNTs did not occur. SEM-EDS and photostability results showed that MO molecules were primarily in HNTs lumen. Adsorption isotherm studies revealed an interesting phenomenon, i.e., a sudden increase of adsorption capacity occurred in the initial dye concentration of about 75 mg/L, which was just the dye concentration corresponding to the onset of dye oligomer formation. This suggested dye aggregation state had a decisive influence to the adsorption behavior of MO on the halloysite. BET results demonstrated at low and high dye concentrations, single MO molecule and aggregation of several dimers through hydrophobic interaction, interacted with Al-OH2+ sites on the inner wall, respectively. Desorption experiments showed that MO in HNTs can be completely removed with deionized water, indicating halloysite is a low-cost and efficient adsorbent for anionic dye.

Inter-hospital variations in caesarean sections. A risk adjusted comparison in the Valencia public hospitals

PubMed Central

Librero, J.; Peiro, S.; Calderon, S. M.

2000-01-01

BACKGROUND—The aim of this study was to describe the variability in caesarean rates in the public hospitals in the Valencia Region, Spain, and to analyse the association between caesarean sections and clinical and extra-clinical factors.
METHODS—Analysis of data contained in the Minimum Basic Data Set (MBDS) compiled for all births in 11 public hospitals in Valencia during 1994-1995 (n=36 819). Bivariate and multivariate analyses were used to evaluate the association between caesarean section rates and specific risk factors. The multivariate model was used to construct predictions about caesarean rates for each hospital, for comparison with rates observed.
RESULTS—Caesarean rates were 17.6% (inter-hospital range: 14.7% to 25.0%), with ample variability between hospitals in the diagnosis of maternal-fetal risk factors (particularly dystocia and fetal distress), and the indication for caesarean in the presence of these factors. Multivariate analysis showed that maternal-fetal risk factors correlated strongly with caesarean section, although extra-clinical factors, such as the day of the week, also correlated positively. After adjusting for the risk factors, the inter-hospital variation in caesarean rates persisted.
CONCLUSIONS—Although certain limitations (imprecision of some diagnoses and information biases in the MBDS) make it impossible to establish unequivocal conclusions, results show a high degree of variability among hospitals when opting for caesarean section. This variability cannot be justified by differences in obstetric risks.


Keywords: hospital utilisation; medical practice variation; caesarean section; administrative databases PMID:10890876

A study of DNA protective effect of orange juice supplementation.

PubMed

Szeto, Yim Tong; To, Tai Lun; Pak, Sok Cheon; Kalle, Wouter

2013-05-01

The potential acute genoprotective effect of orange juice supplementation was investigated. Six healthy subjects (aged 33 to 60 years; 3 women and 3 men) were asked to drink 400 mL of commercial orange juice, which contained 100 mg vitamin C and 40.8 g sugar. Venous blood (2 mL) was taken before and 2 h after ingestion (test trial). A week later, the subjects were asked to repeat the trial by drinking 400 mL water with 100 mg vitamin C and 40.8 g glucose (control trial). Lymphocytes isolated from blood samples underwent comet assay on the day of collection. Pre- and postingestion DNA damage scores were measured in both the test and control trials. Results showed that there was a significant decrease in DNA damage induced by hydrogen peroxide after 2 h of supplementation with orange juice, and no change in baseline DNA damage. There was no significant decrease in the DNA damage in lymphocytes in the control trial.

Post Waterflood CO2 Miscible Flood in Light Oil, Fluvial-Dominated Deltaic Reservoir (Pre-Work and Project Proposal), Class I

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bou-Mikael, Sami

This project outlines a proposal to improve the recovery of light oil from waterflooded fluvial dominated deltaic (FDD) reservoir through a miscible carbon dioxide (CO2) flood. The site is the Port Neches Field in Orange County, Texas. The field is well explored and well exploited. The project area is 270 acres within the Port Neches Field.

A critical review of the epidemiology of Agent Orange/TCDD and prostate cancer.

PubMed

Chang, Ellen T; Boffetta, Paolo; Adami, Hans-Olov; Cole, Philip; Mandel, Jack S

2014-10-01

To inform risk assessment and regulatory decision-making, the relationship between 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and prostate cancer requires clarification. This article systematically and critically reviews the epidemiologic evidence on the association between exposure to TCDD or Agent Orange, a TCDD-contaminated herbicide used during the Vietnam War, and prostate cancer risk. Articles evaluated include 11 studies of three cohorts, four case-control or cross-sectional studies, and three case-only studies of military veterans with information on estimated Agent Orange or TCDD exposure; 13 studies of seven cohorts, one case-control study, and eight proportionate morbidity or mortality studies of Vietnam veterans without information on Agent Orange exposure; 11 cohort studies of workers with occupational exposure to TCDD; and two studies of one community cohort with environmental exposure to TCDD. The most informative studies, including those of Vietnam veterans involved in Agent Orange spraying or other handling, herbicide manufacturing or spraying workers with occupational TCDD exposure, and community members exposed to TCDD through an industrial accident, consistently reported no significant increase in prostate cancer incidence or mortality. Only some potentially confounded studies of Vietnam veterans compared with the general population, studies with unreliable estimates of Agent Orange exposure, and analyses of selected subgroups of Vietnam veterans reported positive associations. Overall, epidemiologic research offers no consistent or convincing evidence of a causal relationship between exposure to Agent Orange or TCDD and prostate cancer. More accurate exposure assessment is needed in large epidemiologic studies to rule out a causal association more conclusively.

Fate and control of pathogenic and spoilage micro-organisms in orange blossom (Citrus aurantium) and rose flower (Rosa centifolia) hydrosols.

PubMed

Labadie, C; Cerutti, C; Carlin, F

2016-12-01

Hydrosols are hydrodistillation products used in food and cosmetic industries, perfumery, pharmacy and aromatherapy. The ability of preservatives to control previously reported bacterial proliferation and spoilage was evaluated. All tested preservatives were authorized for food and cosmetic application. Major pathogens of concern for foods and cosmetics were poorly able to grow in rose and orange blossom hydrosols when inoculated and incubated at 30°C. Commercial antimicrobials, such as isothiazolinone, chlorphenesin and paraben solutions, benzyl alcohol and sodium benzoate at pH = 5·0, controlled the growth of Pseudomonas and Burkholderia sp. strains representative of the natural microbiota of both hydrosols for >90 days at 30°C, only at concentrations close to the authorized limits. Concentrations of some of the tested preservatives that controlled growth at 5°C were lower than at 30°C. Pathogenic micro-organisms likely represent a low risk in rose flower and orange blossom hydrosol. However, the oligotrophic character of hydrosols and the antimicrobial properties of their essential oils do not prevent microbiological spoilage by the naturally present microbiota. In the absence of aseptic conditions and microbial inactivation process, only preservatives can stabilize hydrosols for a several-month storage. Several effective preservatives have been identified. © 2016 The Society for Applied Microbiology.

Vitamin C Content of Commercial Orange Juices

ERIC Educational Resources Information Center

Haddad, Paul

1977-01-01

Describes an experiment designed to confirm that newly purchased commercial orange juice contains sufficient ascorbic acid to meet government standards, and to establish the rate of aerial oxidation of this ascorbic acid when the juice is stored in a refrigerator. (MLH)

Introduction of orange rust caused by Puccinia kuehnii into the Louisiana sugarcane industry

USDA-ARS?s Scientific Manuscript database

The first observation of orange rust infecting sugarcane, caused by Puccinia kuehnii, in the Americas was in Florida in 2007. To monitor for the possible introduction of orange rust into Louisiana, visual surveys were initiated throughout the Louisiana sugarcane industry among plantings of cultivar...

Drinking orange juice increases total antioxidant status and decreases lipid peroxidation in adults.

PubMed

Foroudi, Shahrzad; Potter, Andrew S; Stamatikos, Alexis; Patil, Bhimanagouda S; Deyhim, Farzad

2014-05-01

Cardiovascular disease (CVD) is the leading cause of death in the world and is the primary cause of mortality among Americans. One of the many reasons for the pathogenesis of CVD is attributed to eating diets high in saturated fat and refined carbohydrates and low in fruits and vegetables. Epidemiological evidence has supported a strong association between eating diets rich in fruits and vegetables and cardiovascular health. An experiment was conducted utilizing 24 adults with hypercholesterolemia and hypertriglyceridemia to evaluate the impact of drinking 20 fl oz of freshly squeezed orange juice daily for 90 days on blood pressure, lipid panels, plasma antioxidant capacity, metabolic hormones, lipid peroxidation, and inflammatory markers. Except for addition of drinking orange juice, subjects did not modify their eating habits. The findings suggested that drinking orange juice does not affect (P>.1) blood pressure, lipid panels, metabolic hormones, body fat percentage, or inflammatory markers. However, total plasma antioxidant capacity was significantly increased (P<.05) and lipid peroxidation was significantly decreased (P<.05) after orange juice consumption. Drinking orange juice may protect the cardiovascular system by increasing total plasma antioxidant status and by lowering lipid peroxidation independent of other cardiovascular risk markers evaluated in this study.

Carotenoid accumulation in orange-pigmented Capsicum annuum fruit, regulated at multiple levels

PubMed Central

Rodriguez-Uribe, Laura; Guzman, Ivette; Rajapakse, Wathsala; Richins, Richard D.; O’Connell, Mary A.

2012-01-01

The pericarp of Capsicum fruit is a rich dietary source of carotenoids. Accumulation of these compounds may be controlled, in part, by gene transcription of biosynthetic enzymes. The carotenoid composition in a number of orange-coloured C. annuum cultivars was determined using HPLC and compared with transcript abundances for four carotenogenic enzymes, Psy, LcyB, CrtZ-2, and Ccs determined by qRT-PCR. There were unique carotenoid profiles as well as distinct patterns of transcription of carotenogenic enzymes within the seven orange-coloured cultivars. In one cultivar, ‘Fogo’, carrying the mutant ccs-3 allele, transcripts were detected for this gene, but no CCS protein accumulated. The premature stop termination in ccs-3 prevented expression of the biosynthetic activity to synthesize the capsanthin and capsorubin forms of carotenoids. In two other orange-coloured cultivars, ‘Orange Grande’ and ‘Oriole’, both with wild-type versions of all four carotenogenic enzymes, no transcripts for Ccs were detected and no red pigments accumulated. Finally, in a third case, the orange-coloured cultivar, Canary, transcripts for all four of the wild-type carotenogenic enzymes were readily detected yet no CCS protein appeared to accumulate and no red carotenoids were synthesized. In the past, mutations in Psy and Ccs have been identified as the loci controlling colour in the fruit. Now there is evidence that a non-structural gene may control colour development in Capsicum. PMID:21948863

Hemoglobinopathies and thalassemia screening among Senoi Orang Asli in Peninsular Malaysia

NASA Astrophysics Data System (ADS)

Rong, Danny Koh Xuan; Ismail, Endom; Sabudin, Raja Zahratul Azma Raja; Hussin, Noor Hamidah; Othman, Ainoon

2015-09-01

Orang Asli are the minority indigenous people in Peninsular Malaysia and can be divided into 3 main groups (Negrito, Senoi and Proto Malay) with different six sub-ethnics under each group. Within the Senoi group, the six sub-ethnics are sub-ethnic Mah Meri, Semoq Beri, Che Wong, Jah Hut, Semai and Temiar. This study was aimed to investigate the current prevalence of α- and β-thalassemia and hemoglobinopathies and their mutation types among all six sub-ethnics of Senoi Orang Asli. Blood samples from 685 Senoi participants were collected and sent immediately for routine full blood count analysis and hemoglobin sub-typing. Of 378 subjects screened, 7 subjects were found to be Hemoglobin E (HbE) beta thalassemia carriers, 13 beta thalassaemic, 35 Hemoglobin Constant Spring (HbCS) carriers, 6 compound HbE and HbCS carriers, 32 with HbE disease and 163 HbE heterozygote carriers. The findings of high HbE among Temiars and Jah Huts and high HbCS exclusively in Jah Huts in this study suggest distinct differences across sub-ethnics under Senoi group. Understanding of prevalence and wide spectrum of thalassemia and hemoglobinopathies among Senoi and Orang Asli is essential for national thalassaemia awareness and prevention program, especially in Orang Asli communities.

21 CFR 74.250 - Orange B.

Code of Federal Regulations, 2010 CFR

2010-04-01

... listed in part 73 of this chapter as safe and suitable in color additive mixtures for coloring foods. (b... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Orange B. 74.250 Section 74.250 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR...

77 FR 23659 - Revocation of Antidumping Duty Order: Certain Orange Juice From Brazil

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-20

... Duty Order: Certain Orange Juice From Brazil AGENCY: Import Administration, International Trade...) initiated a sunset review of the antidumping duty order on certain orange juice (OJ) from Brazil.\\1\\ On...), the Department is revoking the antidumping duty order on OJ from Brazil. \\1\\ See Initiation of Five...

Pesticide residues in orange fruit from citrus orchards in Nuevo Leon State, Mexico.

PubMed

Suárez-Jacobo, Angela; Alcantar-Rosales, Victor Manuel; Alonso-Segura, Diana; Heras-Ramírez, Maria; Elizarragaz-De La Rosa, Dalau; Lugo-Melchor, Ofelia; Gaspar-Ramirez, Octavio

2017-09-01

Some international organisations established maximum residue limits (MRLs) in food to protect human health. Mexico lacks regulations in this matter, affecting national and international trade from agroindustry. The aim of this study was to diagnose pesticide residues in oranges from Nuevo Leon, México, in citrus orchards. In May 2014, 100 orange fruit samples were taken randomly from orchards and subjected to analysis for 93 pesticides at residual level by GC/QQQ-MS and LCQ-TOF-MS. Results showed the presence of 15 pesticide residues in the samples. The comparison of the residual levels of pesticides found in orange samples among the MRLs allowed by USA, EU and Japanese regulations demonstrated that all samples were below MRLs issued by USA and Japan. Some orange samples were above MRLs issued by the EU. This provides a basis to establish strategies in order to satisfy International Standards to protect human health and encourage Food Safety in Mexico.

Gene expression in Citrus sinensis fruit tissues harvested from huanglongbing-infected trees: comparison with girdled fruit.

PubMed

Liao, Hui-Ling; Burns, Jacqueline K

2012-05-01

Distribution of viable Candidatus Liberibacter asiaticus (CaLas) in sweet orange fruit and leaves ('Hamlin' and 'Valencia') and transcriptomic changes associated with huanglongbing (HLB) infection in fruit tissues are reported. Viable CaLas was present in most fruit tissues tested in HLB trees, with the highest titre detected in vascular tissue near the calyx abscission zone. Transcriptomic changes associated with HLB infection were analysed in flavedo (FF), vascular tissue (VT), and juice vesicles (JV) from symptomatic (SY), asymptomatic (AS), and healthy (H) fruit. In SY 'Hamlin', HLB altered the expression of more genes in FF and VT than in JV, whereas in SY 'Valencia', the number of genes whose expression was changed by HLB was similar in these tissues. The expression of more genes was altered in SY 'Valencia' JV than in SY 'Hamlin' JV. More genes were also affected in AS 'Valencia' FF and VT than in AS 'Valencia' JV. Most genes whose expression was changed by HLB were classified as transporters or involved in carbohydrate metabolism. Physiological characteristics of HLB-infected and girdled fruit were compared to differentiate between HLB-specific and carbohydrate metabolism-related symptoms. SY and girdled fruit were smaller than H and ungirdled fruit, respectively, with poor juice quality. However, girdling did not cause misshapen fruit or differential peel coloration. Quantitative PCR analysis indicated that many selected genes changed their expression significantly in SY flavedo but not in girdled flavedo. Mechanisms regulating development of HLB symptoms may lie in the host disease response rather than being a direct consequence of carbohydrate starvation.

Insecticidal activity of plant essential oils against the vine mealybug, Planococcus ficus.

PubMed

Karamaouna, Filitsa; Kimbaris, Athanasios; Michaelakis, Alphantonios; Papachristos, Dimitrios; Polissiou, Moschos; Papatsakona, Panagiota; Tsora, Eleanna

2013-01-01

The vine mealybug, Planococcus ficus (Signoret) (Hemiptera: Pseudococcidae), is a pest in grape vine growing areas worldwide. The essential oils from the following aromatic plants were tested for their insecticidal activity against P. ficus: peppermint, Mentha piperita L. (Lamiales: Lamiaceae), thyme-leaved savory, Satureja thymbra L., lavender, Lavandula angustifolia Mill, and basil, Ocimum basilicum L. Essential oils from peels of the following fruits were also tested: lemon, Citrus limon L. (Sapindales: Rutaceae), and orange, C. sinensis L. The reference product was paraffin oil. Bioassays were conducted in the laboratory by using spray applications on grape leaves bearing clusters of P. ficus of one size class, which mainly represented either 3rd instar nymphs or pre-ovipositing adult females. The LC50 values for each essential oil varied depending on the P. ficus life stage but did not significantly differ between 3(rd) instar nymphs and adult females. The LC50 values of the citrus, peppermint, and thyme-leaved savory essential oils ranged from 2.7 to 8.1 mg/mL, and the LC50 values of lavender and basil oil ranged from 19.8 to 22.5 and 44.1 to 46.8 mg/mL, respectively. The essential oils from citrus, peppermint and thymeleaved savory were more or equally toxic compared to the reference product, whereas the lavender and basil essential oils were less toxic than the paraffin oil. No phytotoxic symptoms were observed on grape leaves treated with the citrus essential oils, and low phytotoxicity was caused by the essential oils of lavender, thyme-leaved savory, and mint, whereas the highest phytotoxicity was observed when basil oil was used.

Insecticidal Activity of Plant Essential Oils Against the Vine Mealybug, Planococcus ficus

PubMed Central

Karamaouna, Filitsa; Kimbaris, Athanasios; Michaelakis, Αntonios; Papachristos, Dimitrios; Polissiou, Moschos

2013-01-01

The vine mealybug, Planococcus ficus (Signoret) (Hemiptera: Pseudococcidae), is a pest in grape vine growing areas worldwide. The essential oils from the following aromatic plants were tested for their insecticidal activity against P. ficus: peppermint, Mentha piperita L. (Lamiales: Lamiaceae), thyme-leaved savory, Satureja thymbra L., lavender, Lavandula angustifolia Mill, and basil, Ocimum basilicum L. Essential oils from peels of the following fruits were also tested: lemon, Citrus limon L. (Sapindales: Rutaceae), and orange, C. sinensis L. The reference product was paraffin oil. Bioassays were conducted in the laboratory by using spray applications on grape leaves bearing clusters of P. ficus of one size class, which mainly represented either 3rd instar nymphs or pre-ovipositing adult females. The LC50 values for each essential oil varied depending on the P. ficus life stage but did not significantly differ between 3rd instar nymphs and adult females. The LC50 values of the citrus, peppermint, and thyme-leaved savory essential oils ranged from 2.7 to 8.1 mg/mL, and the LC50 values of lavender and basil oil ranged from 19.8 to 22.5 and 44.1 to 46.8 mg/mL, respectively. The essential oils from citrus, peppermint and thymeleaved savory were more or equally toxic compared to the reference product, whereas the lavender and basil essential oils were less toxic than the paraffin oil. No phytotoxic symptoms were observed on grape leaves treated with the citrus essential oils, and low phytotoxicity was caused by the essential oils of lavender, thyme-leaved savory, and mint, whereas the highest phytotoxicity was observed when basil oil was used. PMID:24766523

Effect of Mandarin Orange Yogurt on Allergic Conjunctivitis Induced by Conjunctival Allergen Challenge.

PubMed

Hara, Yuko; Shiraishi, Atsushi; Sakane, Yuri; Takezawa, Yuki; Kamao, Tomoyuki; Ohashi, Yuichi; Yasunaga, Sho; Sugahara, Takuya

2017-06-01

To evaluate the effects of mandarin orange yogurt containing nobiletin and β-lactoglobulin on the allergic conjunctivitis induced by a conjunctival allergen challenge (CAC). Experiment 1 was performed on 26 asymptomatic patients (age, 25.3 ± 5.3 years) with proven seasonal allergic conjunctivitis due to cedar pollen. We compared the degree of conjunctivitis induced by CAC before and after ingesting mandarin orange yogurt for 2 weeks. Experiment 2 was a double-blind, placebo-controlled trial performed on 31 patients (age, 32.5 ± 12.2 years). A diet containing mandarin orange yogurt was compared to a diet containing yogurt lacking the mandarin orange on the conjunctivitis induced by CAC. The temperature of the inferior bulbar conjunctiva was measured before and 20 minutes after the CAC with an ocular surface thermographer (OST). The degree of conjunctival injection and chemosis was graded by slit-lamp biomicroscopy. The changes in the symptoms were evaluated by a questionnaire. In experiment 1, the scores of redness (3.07 ± 3.03 vs. 1.05 ± 1.70), chemosis (2.84 ± 2.27 vs. 0.81 ± 1.11), itching (4.34 ± 3.05 vs. 1.39 ± 2.12), and temperature (0.73 ± 0.42°C vs. 0.45 ± 0.43°C) were significantly lower (P < 0.001) after a diet of mandarin orange yogurt for 2 weeks. In experiment 2, the scores of redness (1.03 ± 0.18 vs. 1.28 ± 0.52; P = 0.0156), itching (1.93 ± 1.92 vs. 2.82 ± 2.21; P = 0.0133), and surface temperature (0.54 ± 0.21°C vs. 0.31 ± 0.25°C; P < 0.001) were significantly lower in the mandarin orange yogurt group than in the control yogurt group. Mandarin orange yogurt can be an effective nutritional intervention for allergic conjunctivitis.

Detection of Coconut cadang-cadang viroid (CCCVd) in oil palm by reverse transcription loop-mediated isothermal amplification (RT-LAMP).

PubMed

Thanarajoo, Sathis Sri; Kong, Lih Ling; Kadir, Jugah; Lau, Wei Hongi; Vadamalai, Ganesan

2014-06-01

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) detected Coconut cadang-cadang viroid (CCCVd) within 60 min at 60 °C in total nucleic acid extracted from oil palm leaves infected with CCCVd. Positive reactions showed colour change from orange to green in the reaction mix after the addition of fluorescent reagent, and a laddering pattern band on 2% agarose gel electrophoresis. Conventional RT-PCR with LAMP primers produced amplicons with a sequence identical to the 297-nt CCCVd oil palm variant with the primers being specific for CCCVd and not for other viroids such as PSTVd and CEVd. RT-LAMP was found to be rapid and specific for detecting oil palm CCCVd. Copyright © 2014 Elsevier B.V. All rights reserved.

Osage-orange: a pioneering stewardship species

Treesearch

James P. Barnett; James D. Burton

1997-01-01

Osage-orange, a small tree with a number of unique characteristics, played an important role in the settlement of the prairies. One of the more significant contributions was in the use of the species for hedges. The thorny, low-spreading crowns provided excellent fencing when properly main tamed. This use, plus its later used in skelterbelts, exert a continuing...

Clinicopathological features of mycosis fungoides in patients exposed to Agent Orange during the Vietnam War.

PubMed

Jang, Min Soo; Jang, Jun Gyu; Han, Sang Hwa; Park, Jong Bin; Kang, Dong Young; Kim, Sang Tae; Suh, Kee Suck

2013-08-01

There are no reports on the clinicopathological features of mycosis fungoides (MF) among veterans exposed to Agent Orange, one of the herbicides used during the Vietnam War. To evaluate the clinical, histopathological and genotypic findings of Vietnam War veterans with MF and a positive history of exposure to Agent Orange, we performed a comparative clinicopathological study between MF patients with a history of Agent Orange exposure and those without a history of Agent Orange exposure. Twelve Vietnam War veterans with MF were identified. The mean interval from Agent Orange exposure to diagnosis was 24.5 years (range, 9-35). Skin lesions were significantly present on exposed and unexposed areas. Most patients (75%) experienced pruritus (mean visual analog scale score of 6.7). MF was manifested by plaques in 10 patients and by lichenification in five. Histopathological features of most cases were consistent with MF. Biopsy specimens also demonstrated irregular acanthosis (66.7%). In the comparative study, MF patients with a history of Agent Orange exposure differed significantly from those without exposure to Agent Orange in demographic and clinical characteristics. In addition, patients with exposure had an increased tendency for lesions in the exposed area. Notably, our patients showed a higher frequency (33.3%) of mycosis fungoides palmaris et plantaris than in previous studies. Histologically, irregular acanthosis was more frequently observed than ordinary MF. Our results indicate that dermatologists should pay close attention to these clinicopathological differences. Careful assessment of history of exposure to defoliants is warranted in some cases suspicious for MF. © 2013 Japanese Dermatological Association.

Retrotransposons Control Fruit-Specific, Cold-Dependent Accumulation of Anthocyanins in Blood Oranges[W][OA

PubMed Central

Butelli, Eugenio; Licciardello, Concetta; Zhang, Yang; Liu, Jianjun; Mackay, Steve; Bailey, Paul; Reforgiato-Recupero, Giuseppe; Martin, Cathie

2012-01-01

Traditionally, Sicilian blood oranges (Citrus sinensis) have been associated with cardiovascular health, and consumption has been shown to prevent obesity in mice fed a high-fat diet. Despite increasing consumer interest in these health-promoting attributes, production of blood oranges remains unreliable due largely to a dependency on cold for full color formation. We show that Sicilian blood orange arose by insertion of a Copia-like retrotransposon adjacent to a gene encoding Ruby, a MYB transcriptional activator of anthocyanin production. The retrotransposon controls Ruby expression, and cold dependency reflects the induction of the retroelement by stress. A blood orange of Chinese origin results from an independent insertion of a similar retrotransposon, and color formation in its fruit is also cold dependent. Our results suggest that transposition and recombination of retroelements are likely important sources of variation in Citrus. PMID:22427337

7 CFR 944.312 - Orange import regulation.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 8 2014-01-01 2014-01-01 false Orange import regulation. 944.312 Section 944.312 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE FRUITS; IMPORT REGULATIONS § 944...

7 CFR 944.312 - Orange import regulation.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 8 2012-01-01 2012-01-01 false Orange import regulation. 944.312 Section 944.312 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE FRUITS; IMPORT REGULATIONS § 944...

7 CFR 944.312 - Orange import regulation.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 8 2011-01-01 2011-01-01 false Orange import regulation. 944.312 Section 944.312 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE FRUITS; IMPORT REGULATIONS § 944...

7 CFR 944.312 - Orange import regulation.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 8 2013-01-01 2013-01-01 false Orange import regulation. 944.312 Section 944.312 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE FRUITS; IMPORT REGULATIONS § 944...

Orange County Outdoor School: Cabin Leader's Manual.

ERIC Educational Resources Information Center

Orange County Dept. of Education, Santa Ana, CA.

Presented in five sections, the manual furnishes cabin leaders (high school students) with background information concerning philosophy, teaching, objectives, daily schedule, and cabin leader responsibilities in the Orange County Outdoor School program. The welcome section contains the history of the Outdoor School, staff responsibilities,…

Assessment of the effectiveness of orange (Citrus reticulata) peel in the recovery of nickel from electroplating wastewater.

PubMed

Hussein, Rim A

2014-12-01

Wastewater discharged from electroplating industry contains different concentrations of heavy metals, which when released into the environment pose a health hazard to human beings. The aim of this study was to assess the effectiveness of orange peel as an adsorbent in the recovery of Nickel (Ni) from electroplating wastewater. The effectiveness of orange peel as an adsorbent was assessed by determining the optimum conditions of adsorption (adsorbent dose, pH, and contact time), calculating the recovery percentage, and characterizing the orange peel sludge resulting from adsorption/desorption process as being hazardous or not. Under optimum conditions for adsorption, orange peel was found to be an effective adsorbent of Ni from electroplating wastewater. It achieved 59.28% removal of the metal from a solution containing 528 mg/l, at a dose of 60 g/l, at pH 7, and for 1-h contact time. The nickel uptake capacity of orange peel was calculated to be 5.2 mg/g. Using HCl for desorption of adsorbed Ni, a recovery of 44.46% of Ni discharged in the wastewater could be reached. Orange peel resulting from the adsorption/desorption process was characterized as being nonhazardous. Orange peel was found to be effective in the recovery of nearly half of the amount of Ni discharged in electroplating wastewater. Further studies are required to determine (a) the impact of the recovered NiCl2 solution on the quality of the plated product, (b) the effect of activation of orange peel on the adsorption process, and (c) the number of cycles during which orange peel can be reused as an effective adsorbent.

Essential Oils of Plants as Biocides against Microorganisms Isolated from Cuban and Argentine Documentary Heritage

PubMed Central

Borrego, Sofía; Valdés, Oderlaise; Vivar, Isbel; Lavin, Paola; Guiamet, Patricia; Battistoni, Patricia; Gómez de Saravia, Sandra; Borges, Pedro

2012-01-01

Natural products obtained from plants with biocidal activity represent an alternative and useful source in the control of biodeterioration of documentary heritage, without negative environmental and human impacts. In this work, we studied the antimicrobial activity of seven essential oils against microorganisms associated with the biodeterioration of documentary heritage. The essential oils were obtained by steam distillation. The antimicrobial activity was analyzed using the agar diffusion method against 4 strains of fungi and 6 bacterial strains isolated from repositories air and documents of the National Archive of the Republic of Cuba and the Historical Archive of the Museum of La Plata, Argentina. Anise and garlic oils showed the best antifungal activity at all concentrations studied, while oregano oil not only was effective against fungi tested but also prevented sporulation of them all. Orange sweet and laurel oils were ineffective against fungi. Clove, garlic, and oregano oils showed the highest antibacterial activity at 25% against Enterobacter agglomerans and Streptomyces sp., while only clove and oregano oils were effective against Bacillus sp. at all concentrations studied. This study has an important implication for the possible use of the natural products from plants in the control of biodeterioration of documentary heritage. PMID:23762760

Assessing the Effects of Climate Variability on Orange Yield in Florida to Reduce Production Forecast Errors

NASA Astrophysics Data System (ADS)

Concha Larrauri, P.

2015-12-01

Orange production in Florida has experienced a decline over the past decade. Hurricanes in 2004 and 2005 greatly affected production, almost to the same degree as strong freezes that occurred in the 1980's. The spread of the citrus greening disease after the hurricanes has also contributed to a reduction in orange production in Florida. The occurrence of hurricanes and diseases cannot easily be predicted but the additional effects of climate on orange yield can be studied and incorporated into existing production forecasts that are based on physical surveys, such as the October Citrus forecast issued every year by the USDA. Specific climate variables ocurring before and after the October forecast is issued can have impacts on flowering, orange drop rates, growth, and maturation, and can contribute to the forecast error. Here we present a methodology to incorporate local climate variables to predict the USDA's orange production forecast error, and we study the local effects of climate on yield in different counties in Florida. This information can aid farmers to gain an insight on what is to be expected during the orange production cycle, and can help supply chain managers to better plan their strategy.

Post-Chernobyl accident radioactivity measurements in the Comunidad Autonoma de Valencia, Spain.

PubMed

Ortiz, J; Ballesteros, L; Serradell, V

1992-03-01

Increased atmospheric radioactivity after the accident in Chernobyl was first detected on air filters. Measurements were begun in Valencia on May 2, 1986, with the maximum activity being observed around May 3-4, 1986. As a consequence of this accident, annual campaigns of measurements on migrating birds (several species of aquatic birds and song-thrushes) were started. The data corresponding to the campaign immediately after the accident (1986/87) show a generalized contamination (approximately 50% of the measured specimens). Significant levels of 134Cs, 137Cs and 110Agm were found. It is important to note that 110Agm is only present in Aythya ferina. In the successive campaigns in 1988/89 and 1989/91 few samples were found to be contaminated and only 137Cs was identified. Strontium-90 was measured and identified in some specimens, mainly in their bones.

Complete degradation of Orange G by electrolysis in sub-critical water.

PubMed

Yuksel, Asli; Sasaki, Mitsuru; Goto, Motonobu

2011-06-15

Complete degradation of azo dye Orange G was studied using a 500 mL continuous flow reactor made of SUS 316 stainless steel. In this system, a titanium reactor wall acted as a cathode and a titanium plate-type electrode was used as an anode in a subcritical reaction medium. This hydrothermal electrolysis process provides an environmentally friendly route that does not use any organic solvents or catalysts to remove organic pollutants from wastewater. Reactions were carried out from 30 to 90 min residence times at a pressure of 7 MPa, and at different temperatures of 180-250°C by applying various direct currents ranging from 0.5 to 1A. Removal of dye from the product solution and conversion of TOC increased with increasing current value. Moreover, the effect of salt addition on degradation of Orange G and TOC conversion was investigated, because in real textile wastewater, many salts are also included together with dye. Addition of Na(2)CO(3) resulted in a massive degradation of the dye itself and complete mineralization of TOC, while NaCl and Na(2)SO(4) obstructed the removal of Orange G. Greater than 99% of Orange G was successfully removed from the product solution with a 98% TOC conversion. Copyright © 2011 Elsevier B.V. All rights reserved.

Comparative study of flavonoid production in lycopene-accumulated and blonde-flesh sweet oranges (Citrus sinensis) during fruit development.

PubMed

Chen, Jiajing; Zhang, Hongyan; Pang, Yibo; Cheng, Yunjiang; Deng, Xiuxin; Xu, Juan

2015-10-01

Four main flavanone glycosides (FGs) and four main polymethoxylated flavones (PMFs) were determined in fruits of 'Cara Cara' navel orange, 'Seike' navel orange, 'Anliu' and 'Honganliu' sweet orange (Citrus sinensis). No bitter neohesperidosides were detected in the FG profiles, indicating the functional inability of 1,2-rhamnosyltransferase, though relatively high transcription levels were detected in the fruit tissues of 'Anliu' and 'Honganliu' sweet oranges. Different to the FGs, the PMFs only exist abundantly in the peel and decreased gradually throughout fruit development of sweet oranges, suggesting the expression of methylation-related genes accounting for PMF biosynthesis have tissue-specificity. Significant changes in production of the eight flavonoids were found between red-flesh and blonde-flesh sweet oranges, indicating that lycopene accumulation might have direct or indirect effects on the modification of flavonoid biosynthesis in these citrus fruits. Copyright © 2015 Elsevier Ltd. All rights reserved.

Properties of Orange-Pupa-Inducing Factor (OPIF) in the swallowtail butterfly, Papilio xuthus L.

PubMed

Yamanaka, Akira; Adachi, Miwa; Imai, Hiroshi; Uchiyama, Terumasa; Inoue, Moeko; Islam, A T M Fayezul; Kitazawa, Chisato; Endo, Katsuhiko

2006-03-01

Diapause pupae of the swallowtail butterfly Papilio xuthus L. exhibit diapause-green, orange and brownish-orange color polymorphism. Development of orange pupae involves a neuroendocrine factor inducing orange pupa (Orange-Pupa-Inducing Factor, OPIF), which is secreted from the head-thoracic region during late pharate pupal stages, in particular from the ganglia of short-day animals located posteriorly from the second thoracic ganglion2 (TG2). This report describes certain properties of OPIF using bioassays involving ligated abdomens of short-day pharate pupae. Localization of OPIF in the central nervous system of short-day larvae indicated that it was present predominantly in TG2, thoracic ganglion3 (TG3) and abdominal ganglion1 (AG1) complexes. OPIF activity in TG(2,3)-AG1 complexes was over two times higher than in the more posteriorely located ganglia. The developmental profile of OPIF in last instar short-day larvae revealed that OPIF activity in larval ganglia posterior to TG2 became gradually higher as larval growth proceeded, suggesting that OPIF might be accumulated in TG(2,3)-AG(1-7) complexes as larvae prepare for pupal molting. Furthermore, ligated abdomens of short-day larvae developed into pupae of an orange type when a 2% NaCl extract containing OPIF prepared from TG(2,3)-AG(1-7) complexes of long-day larvae was injected into ligated abdomens of short-day pharate pupae, indicating that OPIF is also present in long-day larvae. Additionally, a biochemical investigation using gel filtration chromatography showed that the molecular weight of OPIF was about 10 kDa.

Simultaneous HPLC determination of flavonoids and phenolic acids profile in Pêra-Rio orange juice.

PubMed

Mesquita, E; Monteiro, M

2018-04-01

The aim of this study was to develop and validate an HPLC-DAD method to evaluate the phenolic compounds profile of organic and conventional Pêra-Rio orange juice. The proposed method was validated for 10 flavonoids and 6 phenolic acids. A wide linear range (0.01-223.4μg·g -1 ), good accuracy (79.5-129.2%) and precision (CV≤3.8%), low limits of detection (1-22ng·g -1 ) and quantification (0.7-7.4μg), and overall ruggedness were attained. Good recovery was achieved for all phenolic compounds after extraction and cleanup. The method was applied to organic and conventional Pêra-Rio orange juices from beginning, middle and end of the 2016 harvest. Flavones rutin, nobiletin and tangeretin, and flavanones hesperidin, narirutin and eriocitrin were identified and quantified in all organic and conventional juices. Identity was confirmed by mass spectrometry. Nineteen non-identified phenolic compounds were quantified based on DAD spectra characteristic of the chemical class: 7 cinnamic acid derivatives, 6 flavanones and 6 flavones. The phenolic compounds profile of Pêra-Rio orange juices changed during the harvest; levels increased in organic orange juices, and decreased or were about the same in conventional orange juices. Phenolic compounds levels were higher in organic (0.5-1143.7mg·100g -1 ) than in conventional orange juices (0.5-689.7mg·100g -1 ). PCA differentiated organic from conventional FS and NFC juices, and conventional FCOJ from conventional FS and NFC juices, thus differentiating cultivation and processing. Copyright © 2017. Published by Elsevier Ltd.

Effects of Preheating and Storage Temperatures on Aroma Profile and Physical Properties of Citrus-Oil Emulsions.

PubMed

Yang, Ying; Zhao, Chengying; Tian, Guifang; Lu, Chang; Zhao, Shaojie; Bao, Yuming; McClements, David Julian; Xiao, Hang; Zheng, Jinkai

2017-09-06

Citrus oils are used as good carrier oil for emulsion fabrication due to their special flavor and various health-promoting functions. In this study, the effects of preheating temperature (30, 40, 50, 60, and 70 °C) and storage temperature (4, 25, and 37 °C) on aroma profiles and physical properties of three citrus-oil (i.e., mandarin, sweet orange, and bergamot oils) emulsions were systematically investigated for the first time. The results demonstrated the significant impact of temperature on aroma profile and physical properties. The abundance of d-limonene was found to be the main factor determining the aroma of the three citrus-oil emulsions at different preheating and storage temperatures, while β-linalool and linalyl acetate were important for the aroma of bergamot oil emulsion. Preheating temperature showed a profound impact on the aroma of citrus-oil emulsions, and the aroma of different citrus oil emulsions showed different sensitivity to preheating temperature. Storage temperature was also able to alter the properties of citrus oil emulsions. The higher was the storage temperature, the more alteration of aroma and more instability of the emulsions there was, which could be attributed to the alteration of the oil components and the properties of emulsions. Among all three emulsions, bergamot-oil emulsion was the most stable and exhibited the most potent ability to preserve the aroma against high temperature. Our results would facilitate the application of citrus-oil emulsions in functional foods and beverages.

Characterization of paprika (Capsicum annuum) extract in orange juices by liquid chromatography of carotenoid profiles.

PubMed

Mouly, P P; Gaydou, E M; Corsetti, J

1999-03-01

The carotenoid pigment profiles of authentic pure orange juices from Spain and Florida and an industrial paprika (Capsicum annuum) extract used for food coloring were obtained using reversed-phase liquid chromatography with a C18 packed column and an acetone/methanol/water eluent system. The procedure involving the carotenoid extraction is described. Both retention times and spectral properties using photodiode array detection for characterization of the major carotenoids at 430 and 519 nm are given. The influence of external addition of tangerine juice and/or paprika extract on orange juice color is described using the U.S. Department of Agriculture scale and adulterated orange juice. The procedure for quantitation of externally added paprika extract to orange juice is investigated, and the limit of quantitation, coefficient of variation, and recoveries are determined.

Efficacy of herbal essential oils as insecticide against Aedes aegypti (Linn.), Culex quinquefasciatus (Say) and Anopheles dirus (Peyton and Harrison).

PubMed

Phasomkusolsil, Siriporn; Soonwera, Mayura

2011-09-01

The essential oils of Cananga odorata (ylang ylang), Citrus sinensis (orange), Cymbopogon citratus (lemongrass), Cymbopogon nardus (citronella grass), Eucalyptus citriodora (eucalyptus), Ocimum basilicum (sweet basil) and Syzygium aromaticum (clove), were tested for their insecticide activity against Aedes aegypti, Culex quinquefasciatus and Anopheles dirus using the WHO standard susceptibility test. These were applied in soybean oil at dose of 1%, 5% and 10% (w/v). C. citratus had the KT, values against the three mosquito species tested but the knockdown rates (at 10, 30 and 60 minutes) were lower than some essential oils. C. citratus oil had high insecticidal activity against Ae. aegypti, Cx. quinquefasciatus and An. dirus, with LC50 values of < 0.1, 2.22 and < 0.1%, respectively. Ten percent C. citratus gave the highest mortality rates (100%) 24 hours after application. This study demonstrates the potential for the essential oil of C. citratus to be used as an insecticide against 3 species of mosquitoes.

Application of lemon peel essential oil with edible coating agent to prolong shelf life of tofu and strawberry

NASA Astrophysics Data System (ADS)

Rahmawati, Della; Chandra, Mega; Santoso, Stefanus; Puteri, Maria Gunawan

2017-01-01

The essential oil of sweet orange, lemon, and key lime peel were analyzed for their antimicrobial activity. The antimicrobial activity of each citrus essential oil with different concentration was assessed using broth macro-dilution against Bacillus sp, Eschericia coli, Rhizopus stolonifer, and Botrytis sp which represented specific spoilage microorganism in tofu and fresh strawberry. Among all the citrus peel essential oils tested, lemon peel essential oil with 0.6% concentration showed significant activity as an antimicrobial agent against Escherichia coli and Bacillus sp. In other hand 1% of lemon peel essential oil is also considered to be the best concentration of inhibiting the Rhizopus Stolonifer and Botrytis sp. Lemon peel essential oil which has the highest antimicrobial activity was combined with two different kind of edible coating agents (cassava starch and sodium alginate) and was applied in both tofu and strawberry to observe whether it had possibility to decrease the degradation rate of tofu and strawberry. The addition of 0.6% and 1% lemon peel essential oil with each of edible coating agents was significantly able to reduce the degradation of tofu and fresh strawberry.

Increase in the exposition to floods in the Alicante coast (Valencia region, Spain)

NASA Astrophysics Data System (ADS)

Olcina, J.; Rico, A. M.; Hernandez, M.; Martínez, E.

2009-09-01

During the last two decades, Flood episodes along the Alicante coastline have caused important material losses and general disruptions of everyday life in the municipalities affected. We can speak of an increase in the vulnerability to floods chiefly as a result of an increase in exposition. In turn this increase is fundamentally linked to the nature of the recent urbanization process in this area and the corresponding occupation of flood prone land. The province of Alicante occupies one of the first positions in Europe regarding flood risk (ESPON 2006). Even counting with legislation that regulates land uses, processes leading to the occupation of flood risk areas have proven very difficult to manage. In this sense, the Valencia region has enacted legislation such as the Land Use Planning Law of 1989, the Law on Urban Growth Regulations of 1994, and the Planning Regulations of 1998. All these legal pieces attempted to prohibit development in flood prone land declaring this land as "non.urban". New laws such as the Law on Land Use Planning and Landscape protection of 2004, the Law on New Urban Land (2004), and the Decree on Landscape (2006) also consider the need to include the flood hazard in planning activities. Furthermore in 1997, the so-called "Cartography of the flood hazard at the regional level" was elaborated. This mapping exercise was the base for the "Territorial Plan for the Reduction of Flood Risks (PATRICOVA) approved in 2003 and currently the main planning tool for flood management in Valencia. On the other hand, the European Directive 60/2007 pointed towards the need to take into consideration the social perception of Flood risks in order to develop integrated actions of risk management. Accordingly we have undertaken 285 interviews in the coastal communities of Alicante, Calpe and Campello (95 interviews each). We have chosen these municipalities for two reasons: first their significance in population and economic activity terms, and second, their

Agrometeorological models for forecasting the qualitative attributes of "Valência" oranges

NASA Astrophysics Data System (ADS)

Moreto, Victor Brunini; Rolim, Glauco de Souza; Zacarin, Bruno Gustavo; Vanin, Ana Paula; de Souza, Leone Maia; Latado, Rodrigo Rocha

2017-11-01

Forecasting is the act of predicting unknown future events using available data. Estimating, in contrast, uses data to simulate an actual condition. Brazil is the world's largest producer of oranges, and the state of São Paulo is the largest producer in Brazil. The "Valência" orange is among the most common cultivars in the state. We analyzed the influence of monthly meteorological variables during the growth cycle of Valência oranges grafted onto "Rangpur" lime rootstocks (VACR) for São Paulo, and developed monthly agrometeorological models for forecasting the qualitative attributes of VACR in mature orchard. For fruits per box for all months, the best accuracy was of 0.84 % and the minimum forecast range of 4 months. For the relation between °brix and juice acidity (RATIO) the best accuracy was of 0.69 % and the minimum forecast range of 5 months. Minimum, mean and maximum air temperatures, and relative evapotranspiration were the most important variables in the models.

Use of Physio-Hydrological Units for SMOS Validation at the Valencia Anchor Station Study Area

NASA Astrophysics Data System (ADS)

Millán-Scheiding, C.; Antolín, C.; Marco, J.; Soriano, M. P.; Torre, E.; Requena, F.; Carbó, E.; Cano, A.; Lopez-Baeza, E.

2009-04-01

The SMOS space mission will soil moisture over the continents and ocean surface salinity with the sufficient resolution to be used in global climate change studies. With the aim of validating SMOS land data and products at the Valencia Anchor Station site (VAS) in a Mediterranean Ecosystem area of Spain, we have designed a sample methodology using a subdivision of the landscape in environmental units related to the spatial variability of soil moisture (Millán-Scheiding, 2006; Lopez-Baeza, et al. 2008). These physio-hydrological units are heterogeneously structured entities which present a certain degree of internal uniformity of hydrological parameters. The units are delimited by integrating areas with the same physio-morphology, soil type, vegetation, geology and topography (Flugel, et al 2003; Millán-Scheiding et al, 2007). Each of these units presented over the same pedological characteristics, vegetation cover, and landscape position should have a certain degree of internal uniformity in its hydrological parameters and therefore similar soil moisture (SM). The main assumption for each unit is that the dynamical variation of the hydrological parameters within one unit should be minimum compared to the dynamics of another unit. This methodology will hopefully provide an effective sampling design consisting of a reduced number of measuring points, sparsely distributed over the area, or alternatively, using SM validation networks where each sampling point is located where it is representative of the mean soil moisture of a complete unit area. The Experimental Plan for the SMOS Validation Rehearsal Campaign at the VAS area of April-May 2008 used this environmental subdivision in the selection and sampling of over 21.000 soil moisture points in a control area of 10 x 10 km2. The ground measurements were carried out during 4 nights corresponding to a drying out period of the soil. The sampling consisted of 700 plots with 4 volumetric SM cylinders and 7 Delta-T Theta

Application of ultraviolet-C light on oranges for the inactivation of postharvest wound pathogens

USDA-ARS?s Scientific Manuscript database

Germicidal effects of ultraviolet-C (UV-C) light on the postharvest wound pathogens of citrus fruits namely Penicillium digitatum and Penicillium italicum were investigated. P. digitatum and P. italicum spores were inoculated (4.00 – 4.50 log cfu/ orange) onto Washington navel oranges (Citrus sinens...

Olfactory response of Anastrepha striata (Diptera: Tephritidae) to guava and sweet orange volatiles.

PubMed

Diaz-Santiz, Edvin; Rojas, Julio C; Cruz-López, Leopoldo; Hernández, Emilio; Malo, Edi A

2016-10-01

The behavioral responses of virgin and mated female Anastrepha striata Schiner (Diptera: Tephritidae) to guava (Psidium guajava L.) or sweet orange (Citrus sinensis L.) were evaluated separately using multilure traps in two-choice tests in field cages. The results showed that flies were more attracted to guava and sweet orange volatiles than to control (unbaited trap). The physiological state (virgin or mated) of females did not affect their attraction to the fruit volatiles. Combined analysis of gas chromatography coupled with electroantennography (GC-EAD) of volatile extracts of both fruits showed that 1 and 6 compounds from orange and guava, respectively elicited repeatable antennal responses from mated females. The EAD active compounds in guava volatile extracts were identified by gas chromatography-mass spectrometry (GC-MS) as ethyl butyrate, (Z)-3-hexenol, hexanol, ethyl hexanoate, hexyl acetate, and ethyl octanoate. Linalool was identified as the only antennal active compound in sweet orange extracts. In field cage tests, there were no significant differences between the number of mated flies captured by the traps baited with guava extracts and the number caught by traps baited with the 6-component blend that was formulated according to the relative proportions in the guava extracts. Similar results occurred when synthetic linalool was evaluated against orange extracts. From a practical point of view, the compounds identified in this study could be used for monitoring A. striata populations. © 2015 Institute of Zoology, Chinese Academy of Sciences.

76 FR 35886 - Orange Cove Irrigation District, and Friant Power Authority; Notice of Availability of...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-20

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project No. 11068-014--California] Orange Cove Irrigation District, and Friant Power Authority; Notice of Availability of Environmental... has prepared an Environmental Assessment (EA) regarding Orange Cove Irrigation District's and Friant...

Fresh and Commercially Pasteurized Orange Juice: An Analysis of the Metabolism of Flavonoid Compounds

USDA-ARS?s Scientific Manuscript database

Orange juice is a rich source of flavonoids, mainly the flavanones hesperidin and narirutin, associated with health benefits in humans. The objective of this study was to analyze the uptake of flavonoids in humans after the consumption of two types of orange juice, fresh squeezed (fresh juice, FJ) a...

Preparation and luminescence properties of orange-red Ba3Y(PO4)3:Sm3+ phosphors

NASA Astrophysics Data System (ADS)

Xu, Qiguang; Xu, Denghui; Sun, Jiayue

2015-04-01

Ba3Y(PO4)3:Sm3+ phosphors were prepared by a high temperature solid-state reaction in air. X-ray diffraction (XRD), photoluminescence spectra and temperature-dependent emission spectra were utilized to characterize the structure and luminescence properties of the as-prepared phosphor. The results show that the phosphor can be efficiently excited by ultraviolet light and emit a satisfactory orange-red performance, nicely, fitting in well with the widely used UV LED chip. Under 403 nm excitation, the 4G5/2 → 6HJ (J = 5/2, 7/2, 9/2, and 11/2) emissions of Sm3+ are obviously observed. The optimum doping concentration is 5 mol% and corresponding quenching behavior is ascribed to be electric dipole-dipole interaction according to Dexter's theory. The temperature dependent luminescence of Ba3Y(PO4)3:Sm3+ phosphor is also discussed, and the activation energy for thermal quenching is calculated as 0.34 eV. Furthermore, the chromaticity coordinates of Ba3Y(PO4)3:Sm3+ phosphor are calculated to be (0.5558, 0.4380) and the lifetime values of Ba3Y0.995(PO4)3:0.005Sm3+ was 2.45 ms.

[Effectiveness of antimicrobial formulations for acne based on orange (Citrus sinensis) and sweet basil (Ocimum basilicum L) essential oils].

PubMed

Matiz, Germán; Osorio, María R; Camacho, Francisco; Atencia, Maira; Herazo, Jennifer

2012-01-01

Currently, the antimicrobial resistance has developed in bacterial strains involved in the development of acne. Therefore, alternatives to antibiotic treatment have become necessary. Gel formulations were designed based on essential oils and acetic acid, and their effectiveness was evaluated in patients affected by acne. Masked simple experimental study of three gel formulations on 28 volunteer patients, separated in four groups of seven patients. Treatments were applied daily for eight weeks and consisted of (1) antibacterial (essential oils), (2) keratolytic medication (3) essential oils mixed with acetic acetic, and (4) kerolytic medication with acetic acid. Weekly checks were conducted to evaluate patient improvement. All groups reported an improvement of the acne condition, which ranged between 43% and 75% clearance of lesions. Evidence of treatment disappeared within minutes, showing little discomfort or side effects after application. The essential oil formulations were chemically and physically stable during application of treatments. This was demonstrated by gas chromatography, where no evidence no change neither the composition profiles of essential oils nor in acetic acid. The results were ranked good to excellent, particularly for the acetic acid mixture, which achieved improvements of 75%. This appeared to be a result of their joint antiseptic and keratolytic activity. Side effects (burning and redness) disappeared within a few minutes of completing the application, therefore, did not interfere with adherence to treatment.

Strengthening the Role of Part-Time Faculty in Community Colleges. Example Job Description for Part-Time Faculty: Valencia College--Job Description and Essential Competencies

ERIC Educational Resources Information Center

Center for Community College Student Engagement, 2013

2013-01-01

In an effort to support college conversations regarding strengthening the role of part-time faculty, this brief document presents the job description for a Valencia College part-time/adjunct professor (revised as of July 19, 2013). The description includes essential functions, qualifications, and knowledge, skills, and abilities. This is followed…

Health benefits of orange juice and citrus flavonoids

USDA-ARS?s Scientific Manuscript database

The main flavonoids found in orange juice are hesperidin and naringenin, which can affect several metabolic routes that improve blood serum antioxidant capacity and anti-inflammatory performance, while decreasing insulin resistance protecting against diabetes and metabolic syndrome. In addition, or...

OPAC (orange peel activated carbon) derived from waste orange peel for the adsorption of chlorophenoxyacetic acid herbicides from water: Adsorption isotherm, kinetic modelling and thermodynamic studies.

PubMed

Pandiarajan, Aarthi; Kamaraj, Ramakrishnan; Vasudevan, Sudharshan; Vasudevan, Subramanyan

2018-08-01

This study presents the orange peel activated carbon (OPAC), derived from biowaste precursor (orange peel) by single step pyrolysis method and its application for the adsorption of chlorophenoxyacetic acid herbicides from the water. The OPAC exhibited the surface area of 592.471 m 2  g -1 , pore volume and pore diameter of 0.242 cc g -1 and 1.301 nm respectively. The adsorption kinetics and thermodynamic equilibrium modelling for all chlorophenoxyacetic acid herbicides were investigated. The various parametric effects such as pH and temperature were evaluated. A pseudo-second-order kinetic model was well fitted for all the herbicides. The Langmuir isotherm was obeyed for all the herbicides and the maximum Langmuir capacity of 574.71 mg g -1 was achieved. The thermodynamic studies revealed that the adsorption increases with increase in temperature. The results shows that the orange peel derived carbon (OPAC) as effective and efficient adsorbent material for the removal of chlorophenoxyacid herbicides from the water. Copyright © 2018 Elsevier Ltd. All rights reserved.

Simultaneous classification of Oranges and Apples Using Grover's and Ventura' Algorithms in a Two-qubits System

NASA Astrophysics Data System (ADS)

Singh, Manu Pratap; Radhey, Kishori; Kumar, Sandeep

2017-08-01

In the present paper, simultaneous classification of Orange and Apple has been carried out using both Grover's iterative algorithm (Grover 1996) and Ventura's model (Ventura and Martinez, Inf. Sci. 124, 273-296, 2000) taking different superposition of two- pattern start state containing Orange and Apple both, one- pattern start state containing Apple as search state and another one- pattern start state containing Orange as search state. It has been shown that the exclusion superposition is the most suitable two- pattern search state for simultaneous classification of pattern associated with Apples and Oranges and the superposition of phase-invariance are the best choice as the respective search state based on one -pattern start-states in both Grover's and Ventura's methods of classifications of patterns.

Complex layering of the Orange Mountain Basalt: New Jersey, USA

NASA Astrophysics Data System (ADS)

Puffer, John H.; Block, Karin A.; Steiner, Jeffrey C.; Laskowich, Chris

2018-06-01

The Orange Mountain Basalt of New Jersey is a Mesozoic formation consisting of three units: a single lower inflated sheet lobe about 70 m thick (OMB1), a middle pillow basalt about 10 to 20 m thick (OMB2), and an upper compound pahoehoe flow about 20 to 40 m thick (OMB3). The Orange Mountain Basalt is part of the Central Atlantic Magmatic Province. Quarry and road-cut exposures of OMB1 near Paterson, New Jersey, display some unusual layering that is the focus of this study. OMB1 exposures displays the typical upper crust, core, and basal crust layers of sheet lobes but throughout the Patterson area also display distinct light gray layers of microvesicular basalt mineralized with albite directly over the basal crust and under the upper crust. The lower microvesicular layer is associated with mega-vesicular diapirs. We propose that the upper and lower microvesicular layers were composed of viscous crust that was suddenly quenched before it could devolatilize immediately before the solidification of the core. During initial cooling, the bottom of the basal layer was mineralized with high concentrations of calcite and albite during a high-temperature hydrothermal event. Subsequent albitization, as well as zeolite, prehnite, and calcite precipitation events, occurred during burial and circulation of basin brine heated by recurring Palisades magmatism below the Orange Mountain Basalt. Some of the events experienced by the Orange Mountain Basalt are unusual and place constraints on the fluid dynamics of thick flood basalt flows in general. The late penetration of vesicular diapirs through the entire thickness of the flow interior constrains its viscosity and solidification history.

Improved photocatalytic degradation of Orange G using hybrid nanofibers

NASA Astrophysics Data System (ADS)

Ledwaba, Mpho; Masilela, Nkosiphile; Nyokong, Tebello; Antunes, Edith

2017-05-01

Functionalised electrospun polyamide-6 (PA-6) nanofibres incorporating gadolinium oxide nanoparticles conjugated to zinc tetracarboxyphenoxy phthalocyanine (ZnTCPPc) as the sensitizer were prepared for the photocatalytic degradation of Orange G. Fibres incorporating the phthalocyanine alone or a mixture of the nanoparticles and phthalocyanine were also generated. The singlet oxygen-generating ability of the sensitizer was shown to be maintained within the fibre mat, with the singlet oxygen quantum yields increasing upon incorporation of the magnetic nanoparticles. Consequently, the rate of the photodegradation of Orange G was observed to increase with an increase in singlet oxygen quantum yield. A reduction in the half-lives for the functionalised nanofibres was recorded in the presence of the magnetic nanoparticles, indicating an improvement in the efficiency of the degradation process.

Experimental stations as a tool to teach soil science at the University of Valencia

NASA Astrophysics Data System (ADS)

Cerdà, Artemi

2010-05-01

This paper shows the strategies used at the University of Valencia (Department of Geography. Soil Erosion and Degradation Research Group) to teach soil science at the Geography and Enviromental Science Degrees. The use of the Montesa and El Teularet research stations contribute with a better knowledge on soil science for the students as they can see the measurements carried out in the field. Students visit the stations and contribute to measurements and sampling every season. The use of meteorological stations, erosion plots, soil moisture and soil temperatures probes, and sampling give the students the chances to understand the theoretical approach they use to have. This presentation will show how the students evolve, and how their knowledge in soil science is improved.

Skylab beverage container filled with orange juice held by Astronaut Conrad

NASA Technical Reports Server (NTRS)

1973-01-01

An accordian-style beverage dispenser filled with orange juice is held by Astronaut Charles Conrad Jr., Skylab 2 commander, in this close-up view which is a reproduction taken from a color television transmission made by a TV camera aboard the Skylab 1 and 2 space station cluster in Earth orbit. Conrad (head and face not in view) is seated at the wardroom table in the crew quarters of the Orbital Workshop. The dispenser contained beverage crystals, and Conrad has just added the prescribed amount of water to make the orange drink.

Safety, Efficacy, and Mechanistic Studies Regarding Citrus aurantium (Bitter Orange) Extract and p‐Synephrine

PubMed Central

2017-01-01

Citrus aurantium L. (bitter orange) extracts that contain p‐synephrine as the primary protoalkaloid are widely used for weight loss/weight management, sports performance, appetite control, energy, and mental focus and cognition. Questions have been raised about the safety of p‐synephrine because it has some structural similarity to ephedrine. This review focuses on current human, animal, in vitro, and mechanistic studies that address the safety, efficacy, and mechanisms of action of bitter orange extracts and p‐synephrine. Numerous studies have been conducted with respect to p‐synephrine and bitter orange extract because ephedra and ephedrine were banned from use in dietary supplements in 2004. Approximately 30 human studies indicate that p‐synephrine and bitter orange extracts do not result in cardiovascular effects and do not act as stimulants at commonly used doses. Mechanistic studies suggest that p‐synephrine exerts its effects through multiple actions, which are discussed. Because p‐synephrine exhibits greater adrenergic receptor binding in rodents than humans, data from animals cannot be directly extrapolated to humans. This review, as well as several other assessments published in recent years, has concluded that bitter orange extract and p‐synephrine are safe for use in dietary supplements and foods at the commonly used doses. Copyright © 2017 The Authors Phytotherapy Research Published by John Wiley & Sons Ltd. PMID:28752649

Molecular cloning, characterization and functional analysis of QRFP in orange-spotted grouper (Epinephelus coioides).

PubMed

Shu, Hu; Chen, Huapu; Liu, Yun; Yang, Lidong; Yang, Yuqing; Zhang, Haifa

2014-10-01

The peptide QRFP plays an important role in the regulation of vertebrate feeding behavior. In this study, we cloned the full length cDNA of a QRFP precursor in a teleost fish, the orange-spotted grouper (Epinephelus coioides). Sequence analysis has shown that the functional regions of QRFP in other vertebrates (QRFP-25 and QRFP-7) are conserved in orange-spotted grouper. RT-PCR demonstrated that the pre-processed mRNA of QRFP is widely expressed in orange-spotted grouper. Three days of food deprivation did not change the hypothalamic pre-processed QRFP expression. However, QRFP expression significantly increased when the fish were reefed after three days of fasting. Intraperitoneal injection of QRFP-25 peptide to orange-spotted grouper suppressed expression of orexin, but elevated expression of pro-opiomelanocortin (POMC) in the hypothalamus. We also investigated the effects of QRFP-25 on the expression of reproductive genes. The peptide suppressed the expression of seabream-type gonadotropin-releasing hormones (sbGnRH), luteinizing hormone beta subunit (LHβ) and follicle-stimulating hormone beta subunit (FSHβ) in vivo, as well as inhibited the expression of LHβ and FSHβ in pituitary cells in primary culture. Our results indicate that QRFP may play an inhibitory role in the regulation of feeding behavior and reproduction in orange-spotted grouper. Copyright © 2014 Elsevier Inc. All rights reserved.

Excited state characteristics of acridine dyes: acriflavine and acridine orange.

PubMed

Sharma, Vijay K; Sahare, P D; Rastogi, Ramesh C; Ghoshal, S K; Mohan, D

2003-06-01

The magnitude of the Stokes shift (frequency shifts in absorption and fluorescence spectra) is observed on changing the solvents and further has been used to calculate experimentally the dipole moments (ground state and excited state) of acriflavine and acridine orange dye molecules. Theoretically, dipole moments are calculated using PM 3 Model. The dipole moments of excited states, for both molecules investigated here, are higher than the corresponding values in the ground states. The increase in the dipole moment has been explained in terms of the nature of the excited state. Acriflavine dye overcomes the non-lasing behaviour of acridine orange due to quaternization of the central nitrogen atom.

Use of Banana (Musa acuminata Colla AAA) Peel Extract as an Antioxidant Source in Orange Juices.

PubMed

Ortiz, Lucía; Dorta, Eva; Gloria Lobo, M; González-Mendoza, L Antonio; Díaz, Carlos; González, Mónica

2017-03-01

Using banana peel extract as an antioxidant in freshly squeezed orange juices and juices from concentrate was evaluated. Free radical scavenging capacity increased by adding banana peel extracts to both types of orange juice. In addition, remarkable increases in antioxidant capacity using 2,2'-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) radical were observed when equal or greater than 5 mg of banana peel extract per ml of freshly squeezed juice was added. No clear effects were observed in the capacity to inhibit lipid peroxidation. Adding 5 mg banana peel extract per ml of orange juice did not substantially modify the physicochemical and sensory characteristics of either type of juice. However, undesirable changes in the sensory characteristics (in-mouth sensations and colour) were detected when equal or greater than 10 mg banana peel extract per ml of orange juice was added. These results confirm that banana peel is a promising natural additive that increases the capacity to scavenge free radicals of orange juice with acceptable sensory and physicochemical characteristics for the consumer.

In vitro bioaccessibility of carotenoids, flavonoids, and vitamin C from differently processed oranges and orange juices [Citrus sinensis (L.) Osbeck].

PubMed

Aschoff, Julian K; Kaufmann, Sabrina; Kalkan, Onur; Neidhart, Sybille; Carle, Reinhold; Schweiggert, Ralf M

2015-01-21

Carotenoid, flavonoid, and vitamin C concentrations were determined in fresh orange segments and a puree-like homogenate derived thereof, as well as freshly squeezed, flash-pasteurized, and pasteurized juices. Lutein and β-cryptoxanthin were slightly degraded during dejuicing, whereas β-carotene levels were retained. Vitamin C levels remained unaffected, whereas flavonoid levels decreased 8-fold upon juice extraction, most likely due to the removal of flavonoid-rich albedo and juice vesicles. Likewise, the presence of such fibrous matrix compounds during in vitro digestion was assumed to significantly lower the total bioaccessibility (BA) of all carotenoids from fresh fruit segments (12%) as compared to juices (29-30%). Mechanical disruption of orange segments prior to digestion did not alter carotenoid BA, whereas pasteurization of the freshly squeezed juice slightly increased BA by 9-11%. In addition to carotenoid BA, the stabilities of hesperidin, narirutin, and vitamin C including dehydroascorbic acid during in vitro digestion were monitored, and applied analytical methods were briefly validated.

Effective Population Size Dynamics and the Demographic Collapse of Bornean Orang-Utans

PubMed Central

Goossens, Benoit; Nater, Alexander; Morf, Nadja; Salmona, Jordi; Bruford, Michael W.; Van Schaik, Carel P.; Krützen, Michael; Chikhi, Lounès

2012-01-01

Bornean orang-utans experienced a major demographic decline and local extirpations during the Pleistocene and Holocene due to climate change, the arrival of modern humans, of farmers and recent commercially-driven habitat loss and fragmentation. The recent loss of habitat and its dramatic fragmentation has affected the patterns of genetic variability and differentiation among the remaining populations and increased the extinction risk of the most isolated ones. However, the contribution of recent demographic events to such genetic patterns is still not fully clear. Indeed, it can be difficult to separate the effects of recent anthropogenic fragmentation from the genetic signature of prehistoric demographic events. Here, we investigated the genetic structure and population size dynamics of orang-utans from different sites. Altogether 126 individuals were analyzed and a full-likelihood Bayesian approach was applied. All sites exhibited clear signals of population decline. Population structure is known to generate spurious bottleneck signals and we found that it does indeed contribute to the signals observed. However, population structure alone does not easily explain the observed patterns. The dating of the population decline varied across sites but was always within the 200–2000 years period. This suggests that in some sites at least, orang-utan populations were affected by demographic events that started before the recent anthropogenic effects that occurred in Borneo. These results do not mean that the recent forest exploitation did not leave its genetic mark on orang-utans but suggests that the genetic pool of orang-utans is also impacted by more ancient events. While we cannot identify the main cause for this decline, our results suggests that the decline may be related to the arrival of the first farmers or climatic events, and that more theoretical work is needed to understand how multiple demographic events impact the genome of species and how we can assess

Acridine orange as a biosensitive photovoltaic material

NASA Astrophysics Data System (ADS)

Sharifi, Faranak; Bauld, Reg; Fanchini, Giovanni

2013-10-01

Acridine orange (AO), a biosensitive molecule that is customarily used for labeling nucleic acids including DNA and RNA, is here investigated as a cost effective, water soluble, and photoactive material for the fabrication of potentially biosensitive organic photovoltaics. The electronic energy levels of AO are determined using Kelvin Probe Force Microscopy (KPFM) and UV-Visible spectroscopy. The effect of anticrystallization agents, as well as low-temperature annealing, on the work function of AO is investigated: amorphous AO films are shown to possess a significantly higher work function than microcrystalline AO films and the work function also increases by annealing. Photo-induced processes in AO films are investigated by considering the changes of the KPFM signal under illumination. We demonstrate that acridine orange is able to photogenerate electron-hole pairs at rates comparable to the most commonly used solar-grade photovoltaic materials, including polythiophenes. In addition, the effect of the morphology of different types of AO thin films spun from different solvents is studied in bilayer photovoltaic devices fabricated from stacks of AO and phenyl-C61-butyric acid methyl ester thin films.

Solar UV exposure of primary schoolchildren in Valencia, Spain.

PubMed

Serrano, María-Antonia; Cañada, Javier; Moreno, Juan Carlos

2011-04-01

To quantify schoolchildren's exposure to ultraviolet erythemal radiation (UVER), personal dosimeters (VioSpor) were used to measure biologically effective ultraviolet (UV) radiation received in the course of their daily school activities. The study took place in two primary schools in Valencia (39°28'N), Spain, for several weeks from March 2008 until May 2009, with two age groups (6-8 years and 10-11 years) and involved about 47 schoolchildren. The median daily UV exposure values for all age groups and solar height intervals considered in the study ranged from 1.31 to 2.11 standard erythemal doses (SEDs). Individual UV exposure was analyzed as a function of age, gender and dosimeter position. Significant statistical differences were found between different age groups, with the younger age group receiving higher statistically significant UVER exposure. It was also found that boys received significantly higher UVER exposure than girls. It was also noted that shoulder dosimeters registered higher readings than wrist dosimeters. Exposure ratio (ER) is defined as the ratio between the personal dose on a selected anatomical site and the corresponding ambient dose on a horizontal plane. The median ER for all age groups and solar height intervals in the study range from 4.5% to 10.7%, with higher values at lower solar heights.

Space-time airborne disease mapping applied to detect specific behaviour of varicella in Valencia, Spain.

PubMed

Iftimi, Adina; Montes, Francisco; Santiyán, Ana Míguez; Martínez-Ruiz, Francisco

2015-01-01

Airborne diseases are one of humanity's most feared sicknesses and have regularly caused concern among specialists. Varicella is an airborne disease which usually affects children before the age of 10. Because of its nature, varicella gives rise to interesting spatial, temporal and spatio-temporal patterns. This paper studies spatio-temporal exploratory analysis tools to detect specific behaviour of varicella in the city of Valencia, Spain, from 2008 to 2013. These methods have shown a significant association between the spatial and the temporal component, confirmed by the space-time models applied to the data. High relative risk of varicella is observed in economically disadvantaged regions, areas less involved in vaccination programmes. Copyright © 2015 Elsevier Ltd. All rights reserved.

HPLC-DAD Phenolic Characterization and Antioxidant Activities of Ripe and Unripe Sweet Orange Peels

PubMed Central

Omoba, Olufunmilayo Sade; Obafaye, Rebeccah Olajumoke; Salawu, Sule Ola; Boligon, Aline Augusti; Athayde, Margareth Linde

2015-01-01

Phenolic compounds of unripe and ripe sweet orange peels were determined using a high-performance liquid chromatography separation method with diode array detector (HPLC-DAD). The in vitro antioxidant properties and the EC50 (concentration required to obtain a 50% antioxidant effect) values were also determined. The predominant phenolic compounds were quercitrin, rutin, and quercetin with values of 18.77 ± 0.01 mg/mL, 18.65 ± 0.03 mg/mL, and 10.39 ± 0.01 mg/mL respectively in unripe orange peel and 22.61 ± 0.01 mg/mL, 17.93 ± 0.03 mg/mL, and 14.03 ± 0.02 mg/mL respectively in ripe orange peel. The antioxidant properties revealed 2,2′-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) diammonium salt (ABTS) scavenging ability of both unripe and ripe orange peels respectively as 14.68 ± 0.01 and 16.89 ± 0.02 mmol TEAC/g, the Ferric Reducing Antioxidant Properties (FRAP) as 70.69 ± 0.01 and 91.38 ± 0.01 mg gallic acid equivalents/100g, total phenol content as 5.27 ± 0.03 and 9.40 ± 0.01 mg gallic acid equivalents/g and total flavonoid content as 3.30 ± 0.30 and 4.20 ± 0.02 mg quercetin equivalent/g. The antioxidant assays showed enhanced potency of extract from ripe orange peel with EC50 values of 2.71 ± 0.03 mg/mL for 2,2-diphenyl-1-picrylhydrazyl (DPPH), 0.67 ± 0.03 mg/mL for hydroxyl radicals (OH*), 0.57 ± 0.02 mg/mL for Fe2+ chelation, and 0.63 ± 0.06 mg/mL for malondialdehyde (MDA), and was more potent than unripe orange peel. PMID:26783839

Essential Oil Variation from Twenty Two Genotypes of Citrus in Brazil-Chemometric Approach and Repellency Against Diaphorina citri Kuwayama.

PubMed

Andrade, Moacir Dos Santos; Ribeiro, Leandro do Prado; Borgoni, Paulo Cesar; Silva, Maria Fátima das Graças Fernandes da; Forim, Moacir Rossi; Fernandes, João Batista; Vieira, Paulo Cezar; Vendramin, José Djair; Machado, Marcos Antônio

2016-06-22

The chemical composition of volatile oils from 22 genotypes of Citrus and related genera was poorly differentiated, but chemometric techniques have clarified the relationships between the 22 genotypes, and allowed us to understand their resistance to D. citri. The most convincing similarities include the synthesis of (Z)-β-ocimene and (E)-caryophyllene for all 11 genotypes of group A. Genotypes of group B are not uniformly characterized by essential oil compounds. When stimulated with odor sources of 22 genotypes in a Y-tube olfactometer D. citri preferentially entered the arm containing the volatile oils of Murraya paniculata, confirming orange jasmine as its best host. C. reticulata × C. sinensis was the least preferred genotype, and is characterized by the presence of phytol, (Z)-β-ocimene, and β-elemene, which were not found in the most preferred genotype. We speculate that these three compounds may act as a repellent, making these oils less attractive to D. citri.

Consumer acceptance of orange juice mixed with water mint leave extract.

PubMed

Ali, Marwa Rashad

2017-10-31

Orange-Mint leaves juice was formulated using different addition percentages of mint leaves extract. The juice was aseptically processed, packed in glass bottles under sterilized environment and sterilized at 70°C for 15 minutes then storage at 5°C for 3 months. The physicochemical, nutritional value, microbiological and sensory characteristics of the juice was evaluated. There is no difference in pH, total soluble solids, ash, and total acidity in all samples moreover, the sample A2, "orange juice with 15% of mint leaves extract" recorded the best values of sensory evaluation, on the other hand, it was recorded lower values of vitamin C and total phenolic compound than sample (C) or (C1) because of increasing in addition% of mint leaves extract to orange juice. The sensory properties of juice samples were not remarkable any changes during storage. The result indicated that the juice was acceptable up to 3 months of storage 5°C. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Regular consumption of fresh orange juice increases human skin carotenoid content.

PubMed

Massenti, Roberto; Perrone, Anna; Livrea, Maria Antonietta; Lo Bianco, Riccardo

2015-01-01

Dermal carotenoids are a good indicator of antioxidant status in the body. This study aimed to determine whether regular consumption of orange juice could increase dermal carotenoids. Two types of orange juice, obtained from regularly (CI) and partially (PRD) irrigated trees, were tested to reveal any possible association between juice and dermal carotenoids. Soluble solids, titratable acidity, and total carotenoids were quantified in the juice; skin carotenoid score (SCS) was assessed by Raman spectroscopy. Carotenoid content was 7.3% higher in PRD than in CI juice, inducing no difference in SCS. In a first trial with daily juice intakes for 25 days, SCS increased linearly (10%) in the individual with higher initial SCS, and exponentially (15%) in the individual with lower initial SCS. In a second trial, SCS showed a 6.5% increase after 18 days of drinking juice every other day, but returned to initial values three days after last intake. Skin carotenoids can be increased by regular consumption of fresh orange juice, while their persistence may depend on the accumulation level, environmental conditions or living habits.

Orange juice as dietary source of antioxidants for patients with hepatitis C under antiviral therapy.

PubMed

Gonçalves, Danielle; Lima, Claudia; Ferreira, Paula; Costa, Paulo; Costa, Angela; Figueiredo, Walter; Cesar, Thais

2017-01-01

Background: HCV causes alterations in liver metabolism, resulting in biochemical and nutritional disorders. Supplementation with antioxidants has been suggested to minimize the diseases effects. Objective: This study assessed whether orange juice, a source of citrus flavonoids and vitamin C, may contribute to the treatment of patients with chronic hepatitis C. Design: Anthropometric, hemodynamic, dietary, and biochemical parameters, CRP and liver enzymes were measured in 43 adult patients of both genders who were diagnosed with chronic hepatitis C and were under antiviral therapy. Twenty-three patients were supplemented with orange juice for eight consecutive weeks, while 20 were enrolled as control group. Results: Following regular use of orange juice, no alterations were found in body mass, fat, and waist circumference. The serum levels of total cholesterol, LDL-cholesterol, CRP and parameters of oxidative stress decreased in the orange juice group. Furthermore, the levels of the liver enzyme AST decreased in those who had high levels before the intervention. Conclusion: The orange juice was a convenient food in the diet of patients due to the increase in antioxidant capacity and decreased inflammation and cholesterol in blood serum, in addition to maintaining body mass, which protect against the harmful effects caused by the chronic hepatitis C virus.​​​.

Orange juice as dietary source of antioxidants for patients with hepatitis C under antiviral therapy

PubMed Central

Gonçalves, Danielle; Lima, Claudia; Ferreira, Paula; Costa, Paulo; Costa, Angela; Figueiredo, Walter; Cesar, Thais

2017-01-01

ABSTRACT Background: HCV causes alterations in liver metabolism, resulting in biochemical and nutritional disorders. Supplementation with antioxidants has been suggested to minimize the diseases effects. Objective: This study assessed whether orange juice, a source of citrus flavonoids and vitamin C, may contribute to the treatment of patients with chronic hepatitis C. Design: Anthropometric, hemodynamic, dietary, and biochemical parameters, CRP and liver enzymes were measured in 43 adult patients of both genders who were diagnosed with chronic hepatitis C and were under antiviral therapy. Twenty-three patients were supplemented with orange juice for eight consecutive weeks, while 20 were enrolled as control group. Results: Following regular use of orange juice, no alterations were found in body mass, fat, and waist circumference. The serum levels of total cholesterol, LDL-cholesterol, CRP and parameters of oxidative stress decreased in the orange juice group. Furthermore, the levels of the liver enzyme AST decreased in those who had high levels before the intervention. Conclusion: The orange juice was a convenient food in the diet of patients due to the increase in antioxidant capacity and decreased inflammation and cholesterol in blood serum, in addition to maintaining body mass, which protect against the harmful effects caused by the chronic hepatitis C virus.​​​ PMID:28469541

Succinic acid production from orange peel and wheat straw by batch fermentations of Fibrobacter succinogenes S85.